PMC:2724026 / 23092-24060 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2724026","sourcedb":"PMC","sourceid":"2724026","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2724026","text":"Clark and co-workers have examined the folding of a number of WT DDs, all of which appear to have complex folding pathways characterised by multiphasic folding and unfolding.39–42 Complex folding behaviour has been proposed, and they have suggested that this might be an intrinsic consequence of the complex all-alpha Greek key topology. This would, if true, be in stark contrast to the relatively simple, conserved folding mechanism observed in all-beta Greek key proteins, where populated intermediates are observed only in the more stable Ig-like domains.43 Our results for FADD DD demonstrate clearly that these domains do not have intrinsically complex folding pathways. FADD DD folds in a simple two-state manner with no stable kinetic or equilibrium intermediate. Nonetheless, as is true for most of these other DDs, the observed rate constant of folding is somewhat lower than might be expected for such all-alpha proteins with relatively low contact orders.44","tracks":[{"project":"2_test","denotations":[{"id":"19362094-12755636-62520530","span":{"begin":177,"end":179},"obj":"12755636"},{"id":"19362094-15273313-62520530","span":{"begin":177,"end":179},"obj":"15273313"},{"id":"19362094-16501221-62520530","span":{"begin":177,"end":179},"obj":"16501221"},{"id":"19362094-17408690-62520530","span":{"begin":177,"end":179},"obj":"17408690"},{"id":"19362094-10508783-62520531","span":{"begin":558,"end":560},"obj":"10508783"},{"id":"19362094-9545386-62520532","span":{"begin":966,"end":968},"obj":"9545386"}],"attributes":[{"subj":"19362094-12755636-62520530","pred":"source","obj":"2_test"},{"subj":"19362094-15273313-62520530","pred":"source","obj":"2_test"},{"subj":"19362094-16501221-62520530","pred":"source","obj":"2_test"},{"subj":"19362094-17408690-62520530","pred":"source","obj":"2_test"},{"subj":"19362094-10508783-62520531","pred":"source","obj":"2_test"},{"subj":"19362094-9545386-62520532","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#a093ec","default":true}]}]}}