PMC:2724026 / 19989-20786 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2724026","sourcedb":"PMC","sourceid":"2724026","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2724026","text":"Downwards curvature in the unfolding arm can formally be caused by: (i) changes in the ground (native) state;32 (ii) movement of the transition state along a broad energy barrier;33,34 or (iii) a high-energy intermediate separating two transitions states that switch due to, for example, mutation or an increase in the concentration of denaturant.35 (See Refs 36 and 37 for detailed discussion of different explanations for curvature.) Note that in both models (ii) and (iii), mutation of a residue induces curvature when it is in a region of the protein that is more structured in a late transition state than in an early transition state. Thus, analysis of the chevron plots should, in principle, allow us to infer consolidation of the protein as it crosses the transition state barrier(s).17,38","tracks":[{"project":"2_test","denotations":[{"id":"19362094-11955013-62520525","span":{"begin":109,"end":111},"obj":"11955013"},{"id":"19362094-9545382-62520526","span":{"begin":182,"end":184},"obj":"9545382"},{"id":"19362094-10350468-62520526","span":{"begin":182,"end":184},"obj":"10350468"},{"id":"19362094-12488101-62520527","span":{"begin":347,"end":349},"obj":"12488101"},{"id":"19362094-11179897-62520528","span":{"begin":367,"end":369},"obj":"11179897"},{"id":"19362094-15930007-62520528","span":{"begin":367,"end":369},"obj":"15930007"},{"id":"19362094-15504412-62520529","span":{"begin":795,"end":797},"obj":"15504412"},{"id":"19362094-10611302-62520529","span":{"begin":795,"end":797},"obj":"10611302"}],"attributes":[{"subj":"19362094-11955013-62520525","pred":"source","obj":"2_test"},{"subj":"19362094-9545382-62520526","pred":"source","obj":"2_test"},{"subj":"19362094-10350468-62520526","pred":"source","obj":"2_test"},{"subj":"19362094-12488101-62520527","pred":"source","obj":"2_test"},{"subj":"19362094-11179897-62520528","pred":"source","obj":"2_test"},{"subj":"19362094-15930007-62520528","pred":"source","obj":"2_test"},{"subj":"19362094-15504412-62520529","pred":"source","obj":"2_test"},{"subj":"19362094-10611302-62520529","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec9593","default":true}]}]}}