PMC:2682197 / 21432-22049 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2682197","sourcedb":"PMC","sourceid":"2682197","source_url":"http://www.ncbi.nlm.nih.gov/pmc/2682197","text":"10.1371/journal.ppat.1000460.g005 Figure 5 M. tuberculosis Rv2623 is a nucleotide-binding USP.\nHigh Performance Liquid Chromatography (HPLC) analysis of endogenously bound nucleotides from purified His6-Rv2623. Nucleotides species were identified based on their specific retention times on the Mono Q HR 5/5 column, represented by peaks in absorbance at 260 nm, which correspond to that of nucleotide standards (not shown). Bound nucleotides were extracted by boiling, and separated and quantified from a standard curve that relates absorbance peak area to the known amount of ATP injected onto the column (inset).\n\n","divisions":[{"label":"Title","span":{"begin":44,"end":96}}],"tracks":[]}