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Figure 5 Fluticasone propionate does not affect GATA-3 nuclear export.\n(A) Western blot analysis showing that the nuclear export inhibitor leptomycin B (2 nM) does not affect the ability of FP (10−8 M) to prevent anti-CD3/CD28–stimulated GATA-3 nuclear localization measured at 60 min. ***p\u003c0.001 compared to unstimulated cells, ### p\u003c0.001 compared to anti-CD3/CD28–stimulated cells. 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Figure 5 Fluticasone propionate does not affect GATA-3 nuclear export.\n(A) Western blot analysis showing that the nuclear export inhibitor leptomycin B (2 nM) does not affect the ability of FP (10−8 M) to prevent anti-CD3/CD28–stimulated GATA-3 nuclear localization measured at 60 min. ***p\u003c0.001 compared to unstimulated cells, ### p\u003c0.001 compared to anti-CD3/CD28–stimulated cells. (B) Western blot analysis showing that FP (10−8 M) does not affect whole-cell GATA-3 degradation over 17 h. (C) GFP-tagged GATA-3 is overexpressed and cells stimulated (b–j) or not (a) with anti-CD3/CD28. The effect of treating cells with FP (10−8 M, f–j) after 30 min stimulation with anti-CD3/CD28 is also shown. (D) FP (10−8 M) does not prevent anti-CD3/CD28–stimulated p65 nuclear translocation at 60 min after stimulation. **p\u003c0.01 compared to unstimulated cells. All results are representative of at least four independent experiments and are shown as mean±SEM. Results were analysed by ANOVA followed by Newman-Keuls test.\n\n"}

{"project":"GO-BP","denotations":[{"id":"T19149","span":{"begin":90,"end":104},"obj":"http://purl.obolibrary.org/obo/GO_0051168"},{"id":"T19150","span":{"begin":149,"end":163},"obj":"http://purl.obolibrary.org/obo/GO_0051168"},{"id":"T19151","span":{"begin":288,"end":300},"obj":"http://purl.obolibrary.org/obo/GO_0051179"},{"id":"T19153","span":{"begin":571,"end":590},"obj":"http://purl.obolibrary.org/obo/GO_0031296"},{"id":"T19154","span":{"begin":505,"end":516},"obj":"http://purl.obolibrary.org/obo/GO_0009056"},{"id":"T19155","span":{"begin":571,"end":590},"obj":"http://purl.obolibrary.org/obo/GO_0002672"},{"id":"T19156","span":{"begin":571,"end":590},"obj":"http://purl.obolibrary.org/obo/GO_0045579"},{"id":"T19157","span":{"begin":571,"end":590},"obj":"http://purl.obolibrary.org/obo/GO_0002869"},{"id":"T19158","span":{"begin":571,"end":590},"obj":"http://purl.obolibrary.org/obo/GO_0002904"},{"id":"T19159","span":{"begin":571,"end":590},"obj":"http://purl.obolibrary.org/obo/GO_0050871"},{"id":"T19160","span":{"begin":571,"end":590},"obj":"http://purl.obolibrary.org/obo/GO_0030890"}],"text":"10.1371/journal.pmed.1000076.g005 Figure 5 Fluticasone propionate does not affect GATA-3 nuclear export.\n(A) Western blot analysis showing that the nuclear export inhibitor leptomycin B (2 nM) does not affect the ability of FP (10−8 M) to prevent anti-CD3/CD28–stimulated GATA-3 nuclear localization measured at 60 min. ***p\u003c0.001 compared to unstimulated cells, ### p\u003c0.001 compared to anti-CD3/CD28–stimulated cells. (B) Western blot analysis showing that FP (10−8 M) does not affect whole-cell GATA-3 degradation over 17 h. (C) GFP-tagged GATA-3 is overexpressed and cells stimulated (b–j) or not (a) with anti-CD3/CD28. The effect of treating cells with FP (10−8 M, f–j) after 30 min stimulation with anti-CD3/CD28 is also shown. (D) FP (10−8 M) does not prevent anti-CD3/CD28–stimulated p65 nuclear translocation at 60 min after stimulation. **p\u003c0.01 compared to unstimulated cells. All results are representative of at least four independent experiments and are shown as mean±SEM. Results were analysed by ANOVA followed by Newman-Keuls test.\n\n"}

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