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PMC:2674207 / 19780-20721 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T6779 0-10 NN denotes GATA-3-GFP
T6780 11-20 NN denotes Construct
T6781 21-24 DT denotes The
T6782 25-31 NN denotes GATA-3
T6783 32-37 NN denotes clone
T6784 38-39 -LRB- denotes (
T6785 39-47 NN denotes BC003070
T6786 47-48 -RRB- denotes )
T6787 49-57 JJ denotes complete
T6788 58-62 NN denotes cDNA
T6789 63-66 VB denotes was
T6790 67-75 VB denotes obtained
T6791 76-80 IN denotes from
T6792 81-91 NNP denotes Invitrogen
T6793 92-96 NNP denotes Life
T6794 97-109 NNP denotes Technologies
T6795 110-112 IN denotes as
T6796 113-114 DT denotes a
T6797 115-118 JJ denotes 5′-
T6798 119-132 NN denotes EcoRI/3′-XhoI
T6799 133-139 NN denotes insert
T6800 140-142 IN denotes of
T6801 143-149 NN denotes GATA-3
T6802 150-152 IN denotes in
T6803 153-156 DT denotes the
T6804 157-162 NN denotes pOTB7
T6805 163-169 NN denotes vector
T6806 171-177 NN denotes GATA-3
T6807 178-181 VB denotes was
T6808 182-189 VB denotes excised
T6809 190-194 IN denotes from
T6810 195-200 NN denotes pOTB7
T6811 201-206 VB denotes using
T6812 207-211 NN denotes XhoI
T6813 212-221 NN denotes digestion
T6814 222-225 CC denotes and
T6815 226-234 NN denotes pEGFP-C2
T6816 235-236 -LRB- denotes (
T6817 236-244 NNP denotes Clontech
T6818 244-245 -COMMA- denotes ,
T6819 246-267 NNP denotes Saint-Germain-en-Laye
T6820 267-268 -COMMA- denotes ,
T6821 269-275 NNP denotes France
T6822 275-276 -RRB- denotes )
T6823 277-280 VB denotes was
T6824 281-289 VB denotes digested
T6825 290-294 IN denotes with
T6826 295-300 NN denotes BamHI
T6827 302-305 NN denotes DNA
T6828 306-309 VB denotes was
T6829 310-319 VB denotes recovered
T6830 320-322 IN denotes by
T6831 323-329 NN denotes phenol
T6832 330-340 NN denotes extraction
T6833 341-344 CC denotes and
T6834 345-352 NN denotes ethanol
T6835 353-366 NN denotes precipitation
T6836 366-367 -COMMA- denotes ,
T6837 368-371 CC denotes and
T6838 372-376 CC denotes both
T6839 377-380 DT denotes the
T6840 381-387 NN denotes GATA-3
T6841 388-396 NN denotes fragment
T6842 397-400 CC denotes and
T6843 401-404 DT denotes the
T6844 405-413 NN denotes pEGFP-C2
T6845 414-420 NN denotes vector
T6846 421-432 JJ denotes blunt-ended
T6847 433-435 IN denotes by
T6848 436-446 NN denotes incubation
T6849 447-451 IN denotes with
T6850 452-458 NNP denotes Klenow
T6851 459-460 -LRB- denotes (
T6852 460-467 NN denotes Bioline
T6853 468-477 NN denotes Bio-27029
T6854 477-478 -RRB- denotes )
T6855 479-482 IN denotes for
T6856 483-485 CD denotes 30
T6857 486-489 NN denotes min
T6858 490-492 IN denotes at
T6859 493-498 NNP denotes 37°C.
T6860 499-505 NNP denotes Klenow
T6861 506-509 VB denotes was
T6862 510-521 VB denotes inactivated
T6863 522-524 IN denotes by
T6864 525-535 NN denotes incubation
T6865 536-539 IN denotes for
T6866 540-542 CD denotes 10
T6867 543-546 NN denotes min
T6868 547-549 IN denotes at
T6869 550-555 NNP denotes 75°C.
T6870 556-559 NN denotes DNA
T6871 560-563 VB denotes was
T6872 564-573 VB denotes recovered
T6873 574-576 IN denotes by
T6874 577-583 NN denotes phenol
T6875 584-594 NN denotes extraction
T6876 595-598 CC denotes and
T6877 599-606 NN denotes ethanol
T6878 607-620 NN denotes precipitation
T6879 620-621 -COMMA- denotes ,
T6880 622-625 CC denotes and
T6881 626-630 CC denotes both
T6882 631-634 DT denotes the
T6883 635-646 JJ denotes blunt-ended
T6884 647-653 NN denotes GATA-3
T6885 654-662 NN denotes fragment
T6886 663-666 CC denotes and
T6887 667-670 DT denotes the
T6888 671-674 NN denotes GFP
T6889 675-681 NN denotes vector
T6890 682-686 VB denotes were
T6891 687-699 RB denotes subsequently
T6892 700-708 VB denotes digested
T6893 709-713 IN denotes with
T6894 714-719 NN denotes EcoRI
T6895 720-726 IN denotes before
T6896 727-730 DT denotes the
T6897 731-748 JJ denotes 5′-EcoRI/3′–blunt
T6898 749-752 NN denotes end
T6899 753-759 NN denotes GATA-3
T6900 760-768 NN denotes fragment
T6901 769-772 VB denotes was
T6902 773-781 VB denotes inserted
T6903 782-786 IN denotes into
T6904 787-790 DT denotes the
T6905 791-808 NN denotes 5′-EcoRI/3′–blunt
T6906 809-814 VB denotes ended
T6907 815-818 NN denotes GFP
T6908 819-825 NN denotes vector
T6909 827-835 JJ denotes Positive
T6910 836-842 NN denotes clones
T6911 843-847 VB denotes were
T6912 848-857 VB denotes confirmed
T6913 858-860 IN denotes by
T6914 861-870 NN denotes digestion
T6915 871-874 CC denotes and
T6916 875-879 NN denotes size
T6917 880-888 NN denotes analysis
T6918 889-891 IN denotes by
T6919 892-893 CD denotes 1
T6920 893-894 NN denotes %
T6921 895-902 NN denotes agarose
T6922 903-906 NN denotes gel
T6923 907-922 NN denotes electrophoresis
T6924 923-926 CC denotes and
T6925 927-929 IN denotes by
T6926 930-940 NN denotes sequencing
R5815 T6780 T6779 arg1Of Construct,GATA-3-GFP
R5816 T6785 T6784 arg2Of BC003070,(
R5817 T6786 T6784 arg3Of ),(
R5818 T6788 T6781 arg1Of cDNA,The
R5819 T6788 T6782 arg1Of cDNA,GATA-3
R5820 T6788 T6783 arg1Of cDNA,clone
R5821 T6788 T6784 arg1Of cDNA,(
R5822 T6788 T6787 arg1Of cDNA,complete
R5823 T6788 T6789 arg1Of cDNA,was
R5824 T6788 T6790 arg2Of cDNA,obtained
R5825 T6790 T6789 arg2Of obtained,was
R5826 T6790 T6791 arg1Of obtained,from
R5827 T6790 T6795 arg1Of obtained,as
R5828 T6794 T6791 arg2Of Technologies,from
R5829 T6794 T6792 arg1Of Technologies,Invitrogen
R5830 T6794 T6793 arg1Of Technologies,Life
R5831 T6799 T6795 arg2Of insert,as
R5832 T6799 T6796 arg1Of insert,a
R5833 T6799 T6797 arg1Of insert,5′-
R5834 T6799 T6798 arg1Of insert,EcoRI/3′-XhoI
R5835 T6799 T6800 arg1Of insert,of
R5836 T6799 T6802 arg1Of insert,in
R5837 T6801 T6800 arg2Of GATA-3,of
R5838 T6805 T6802 arg2Of vector,in
R5839 T6805 T6803 arg1Of vector,the
R5840 T6805 T6804 arg1Of vector,pOTB7
R5841 T6806 T6807 arg1Of GATA-3,was
R5842 T6806 T6808 arg2Of GATA-3,excised
R5843 T6808 T6807 arg2Of excised,was
R5844 T6808 T6809 arg1Of excised,from
R5845 T6810 T6809 arg2Of pOTB7,from
R5846 T6813 T6811 arg1Of digestion,using
R5847 T6813 T6812 arg1Of digestion,XhoI
R5848 T6813 T6814 arg1Of digestion,and
R5849 T6814 T6823 arg1Of and,was
R5850 T6814 T6824 arg2Of and,digested
R5851 T6815 T6814 arg2Of pEGFP-C2,and
R5852 T6815 T6816 arg1Of pEGFP-C2,(
R5853 T6817 T6818 arg1Of Clontech,","
R5854 T6818 T6820 arg1Of ",",","
R5855 T6819 T6818 arg2Of Saint-Germain-en-Laye,","
R5856 T6820 T6816 arg2Of ",",(
R5857 T6821 T6820 arg2Of France,","
R5858 T6822 T6816 arg3Of ),(
R5859 T6824 T6807 modOf digested,was
R5860 T6824 T6823 arg2Of digested,was
R5861 T6824 T6825 arg1Of digested,with
R5862 T6826 T6825 arg2Of BamHI,with
R5863 T6827 T6828 arg1Of DNA,was
R5864 T6827 T6829 arg2Of DNA,recovered
R5865 T6829 T6828 arg2Of recovered,was
R5866 T6832 T6831 arg1Of extraction,phenol
R5867 T6832 T6833 arg1Of extraction,and
R5868 T6833 T6829 arg1Of and,recovered
R5869 T6833 T6830 arg2Of and,by
R5870 T6835 T6833 arg2Of precipitation,and
R5871 T6835 T6834 arg1Of precipitation,ethanol
R5872 T6837 T6836 arg1Of and,","
R5873 T6841 T6839 arg1Of fragment,the
R5874 T6841 T6840 arg1Of fragment,GATA-3
R5875 T6841 T6842 arg1Of fragment,and
R5876 T6842 T6838 arg1Of and,both
R5877 T6845 T6842 arg2Of vector,and
R5878 T6845 T6843 arg1Of vector,the
R5879 T6845 T6844 arg1Of vector,pEGFP-C2
R5880 T6845 T6846 arg1Of vector,blunt-ended
R5881 T6848 T6847 arg2Of incubation,by
R5882 T6850 T6849 arg2Of Klenow,with
R5883 T6852 T6851 arg2Of Bioline,(
R5884 T6852 T6853 arg1Of Bioline,Bio-27029
R5885 T6854 T6851 arg3Of ),(
R5886 T6857 T6855 arg2Of min,for
R5887 T6857 T6856 arg1Of min,30
R5888 T6860 T6859 arg1Of Klenow,37°C.
R5889 T6860 T6861 arg1Of Klenow,was
R5890 T6860 T6862 arg2Of Klenow,inactivated
R5891 T6862 T6861 arg2Of inactivated,was
R5892 T6862 T6863 arg1Of inactivated,by
R5893 T6864 T6863 arg2Of incubation,by
R5894 T6864 T6865 arg1Of incubation,for
R5895 T6867 T6865 arg2Of min,for
R5896 T6867 T6866 arg1Of min,10
R5897 T6867 T6868 arg1Of min,at
R5898 T6870 T6868 arg2Of DNA,at
R5899 T6870 T6869 arg1Of DNA,75°C.
R5900 T6872 T6871 arg2Of recovered,was
R5901 T6875 T6874 arg1Of extraction,phenol
R5902 T6875 T6876 arg1Of extraction,and
R5903 T6876 T6872 arg1Of and,recovered
R5904 T6876 T6873 arg2Of and,by
R5905 T6878 T6876 arg2Of precipitation,and
R5906 T6878 T6877 arg1Of precipitation,ethanol
R5907 T6880 T6879 arg1Of and,","
R5908 T6885 T6882 arg1Of fragment,the
R5909 T6885 T6883 arg1Of fragment,blunt-ended
R5910 T6885 T6884 arg1Of fragment,GATA-3
R5911 T6885 T6886 arg1Of fragment,and
R5912 T6886 T6881 arg1Of and,both
R5913 T6886 T6890 arg1Of and,were
R5914 T6886 T6892 arg2Of and,digested
R5915 T6889 T6886 arg2Of vector,and
R5916 T6889 T6887 arg1Of vector,the
R5917 T6889 T6888 arg1Of vector,GFP
R5918 T6892 T6880 arg2Of digested,and
R5919 T6892 T6890 arg2Of digested,were
R5920 T6892 T6891 arg1Of digested,subsequently
R5921 T6892 T6893 arg1Of digested,with
R5922 T6892 T6895 arg1Of digested,before
R5923 T6894 T6893 arg2Of EcoRI,with
R5924 T6900 T6896 arg1Of fragment,the
R5925 T6900 T6897 arg1Of fragment,5′-EcoRI/3′–blunt
R5926 T6900 T6898 arg1Of fragment,end
R5927 T6900 T6899 arg1Of fragment,GATA-3
R5928 T6900 T6901 arg1Of fragment,was
R5929 T6900 T6902 arg2Of fragment,inserted
R5930 T6902 T6895 arg2Of inserted,before
R5931 T6902 T6901 arg2Of inserted,was
R5932 T6902 T6903 arg1Of inserted,into
R5933 T6906 T6905 arg1Of ended,5′-EcoRI/3′–blunt
R5934 T6908 T6903 arg2Of vector,into
R5935 T6908 T6904 arg1Of vector,the
R5936 T6908 T6906 arg1Of vector,ended
R5937 T6908 T6907 arg1Of vector,GFP
R5938 T6910 T6909 arg1Of clones,Positive
R5939 T6910 T6911 arg1Of clones,were
R5940 T6910 T6912 arg2Of clones,confirmed
R5941 T6912 T6911 arg2Of confirmed,were
R5942 T6912 T6918 arg1Of confirmed,by
R5943 T6912 T6925 arg1Of confirmed,by
R5944 T6914 T6915 arg1Of digestion,and
R5945 T6916 T6915 arg2Of size,and
R5946 T6917 T6912 arg1Of analysis,confirmed
R5947 T6917 T6913 arg2Of analysis,by
R5948 T6917 T6914 arg1Of analysis,digestion
R5949 T6917 T6916 arg1Of analysis,size
R5950 T6918 T6924 arg1Of by,and
R5951 T6919 T6920 arg1Of 1,%
R5952 T6923 T6918 arg2Of electrophoresis,by
R5953 T6923 T6919 arg1Of electrophoresis,1
R5954 T6923 T6921 arg1Of electrophoresis,agarose
R5955 T6923 T6922 arg1Of electrophoresis,gel
R5956 T6925 T6924 arg2Of by,and
R5957 T6926 T6925 arg2Of sequencing,by

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T6937 0-10 NNP denotes GATA-3-GFP
T6938 11-20 NNP denotes Construct
T6939 21-24 NNP denotes The
T6940 25-31 NNP denotes GATA-3
T6941 32-37 NN denotes clone
T6942 38-39 -LRB- denotes (
T6943 39-47 NNP denotes BC003070
T6944 47-48 -RRB- denotes )
T6945 49-57 JJ denotes complete
T6946 58-62 NNP denotes cDNA
T6947 63-66 VBD denotes was
T6948 67-75 VBN denotes obtained
T6949 76-80 IN denotes from
T6950 81-91 NNP denotes Invitrogen
T6951 92-96 NNP denotes Life
T6952 97-109 NNPS denotes Technologies
T6953 110-112 IN denotes as
T6954 113-114 DT denotes a
T6955 115-116 CD denotes 5
T6956 116-117 SYM denotes ′
T6957 117-118 : denotes -
T6958 119-126 JJ denotes EcoRI/3
T6959 126-127 SYM denotes ′
T6960 127-128 : denotes -
T6961 128-132 NNP denotes XhoI
T6962 133-139 NN denotes insert
T6963 140-142 IN denotes of
T6964 143-149 NN denotes GATA-3
T6965 150-152 IN denotes in
T6966 153-156 DT denotes the
T6967 157-162 NNP denotes pOTB7
T6968 163-169 NN denotes vector
T6969 169-170 . denotes .
T6970 171-177 NN denotes GATA-3
T6971 178-181 VBD denotes was
T6972 182-189 VBN denotes excised
T6973 190-194 IN denotes from
T6974 195-200 NNP denotes pOTB7
T6975 201-206 VBG denotes using
T6976 207-211 NNP denotes XhoI
T6977 212-221 NN denotes digestion
T6978 222-225 CC denotes and
T6979 226-234 NN denotes pEGFP-C2
T6980 235-236 -LRB- denotes (
T6981 236-244 NNP denotes Clontech
T6982 244-245 , denotes ,
T6983 246-267 NNP denotes Saint-Germain-en-Laye
T6984 267-268 , denotes ,
T6985 269-275 NNP denotes France
T6986 275-276 -RRB- denotes )
T6987 277-280 VBD denotes was
T6988 281-289 VBN denotes digested
T6989 290-294 IN denotes with
T6990 295-300 NNP denotes BamHI
T6991 300-301 . denotes .
T6992 302-305 NNP denotes DNA
T6993 306-309 VBD denotes was
T6994 310-319 VBN denotes recovered
T6995 320-322 IN denotes by
T6996 323-329 NN denotes phenol
T6997 330-340 NN denotes extraction
T6998 341-344 CC denotes and
T6999 345-352 NN denotes ethanol
T7000 353-366 NN denotes precipitation
T7001 366-367 , denotes ,
T7002 368-371 CC denotes and
T7003 372-376 DT denotes both
T7004 377-380 DT denotes the
T7005 381-387 NNP denotes GATA-3
T7006 388-396 NN denotes fragment
T7007 397-400 CC denotes and
T7008 401-404 DT denotes the
T7009 405-413 NN denotes pEGFP-C2
T7010 414-420 NN denotes vector
T7011 421-432 JJ denotes blunt-ended
T7012 433-435 IN denotes by
T7013 436-446 NN denotes incubation
T7014 447-451 IN denotes with
T7015 452-458 NNP denotes Klenow
T7016 459-460 -LRB- denotes (
T7017 460-467 NNP denotes Bioline
T7018 468-477 NNP denotes Bio-27029
T7019 477-478 -RRB- denotes )
T7020 479-482 IN denotes for
T7021 483-485 CD denotes 30
T7022 486-489 NN denotes min
T7023 490-492 IN denotes at
T7024 493-495 CD denotes 37
T7025 495-496 CD denotes °
T7026 496-498 NNP denotes C.
T7027 499-505 NNP denotes Klenow
T7028 506-509 VBD denotes was
T7029 510-521 VBN denotes inactivated
T7030 522-524 IN denotes by
T7031 525-535 NN denotes incubation
T7032 536-539 IN denotes for
T7033 540-542 CD denotes 10
T7034 543-546 NN denotes min
T7035 547-549 IN denotes at
T7036 550-552 CD denotes 75
T7037 552-553 CD denotes °
T7038 553-555 NNP denotes C.
T7039 556-559 NNP denotes DNA
T7040 560-563 VBD denotes was
T7041 564-573 VBN denotes recovered
T7042 574-576 IN denotes by
T7043 577-583 NN denotes phenol
T7044 584-594 NN denotes extraction
T7045 595-598 CC denotes and
T7046 599-606 NN denotes ethanol
T7047 607-620 NN denotes precipitation
T7048 620-621 , denotes ,
T7049 622-625 CC denotes and
T7050 626-630 DT denotes both
T7051 631-634 DT denotes the
T7052 635-646 JJ denotes blunt-ended
T7053 647-653 JJ denotes GATA-3
T7054 654-662 NN denotes fragment
T7055 663-666 CC denotes and
T7056 667-670 DT denotes the
T7057 671-674 NNP denotes GFP
T7058 675-681 NN denotes vector
T7059 682-686 VBD denotes were
T7060 687-699 RB denotes subsequently
T7061 700-708 VBN denotes digested
T7062 709-713 IN denotes with
T7063 714-719 NNP denotes EcoRI
T7064 720-726 IN denotes before
T7065 727-730 DT denotes the
T7066 731-732 CD denotes 5
T7067 732-733 SYM denotes ′
T7068 733-734 : denotes -
T7069 734-741 JJ denotes EcoRI/3
T7070 741-742 NN denotes ′
T7071 743-748 JJ denotes blunt
T7072 749-752 NN denotes end
T7073 753-759 NN denotes GATA-3
T7074 760-768 NN denotes fragment
T7075 769-772 VBD denotes was
T7076 773-781 VBN denotes inserted
T7077 782-786 IN denotes into
T7078 787-790 DT denotes the
T7079 791-792 CD denotes 5
T7080 792-793 SYM denotes ′
T7081 793-794 : denotes -
T7082 794-801 JJ denotes EcoRI/3
T7083 801-802 NN denotes ′
T7084 803-808 JJ denotes blunt
T7085 809-814 VBN denotes ended
T7086 815-818 NNP denotes GFP
T7087 819-825 NN denotes vector
T7088 825-826 . denotes .
T7089 827-835 JJ denotes Positive
T7090 836-842 NNS denotes clones
T7091 843-847 VBD denotes were
T7092 848-857 VBN denotes confirmed
T7093 858-860 IN denotes by
T7094 861-870 NN denotes digestion
T7095 871-874 CC denotes and
T7096 875-879 NN denotes size
T7097 880-888 NN denotes analysis
T7098 889-891 IN denotes by
T7099 892-893 CD denotes 1
T7100 893-894 NN denotes %
T7101 895-902 JJ denotes agarose
T7102 903-906 NN denotes gel
T7103 907-922 NN denotes electrophoresis
T7104 923-926 CC denotes and
T7105 927-929 IN denotes by
T7106 930-940 VBG denotes sequencing
T7107 940-941 . denotes .
R5958 T6937 T6938 compound GATA-3-GFP,Construct
R5959 T6938 T6948 nsubjpass Construct,obtained
R5960 T6939 T6941 det The,clone
R5961 T6940 T6941 compound GATA-3,clone
R5962 T6941 T6938 appos clone,Construct
R5963 T6942 T6943 punct (,BC003070
R5964 T6943 T6941 appos BC003070,clone
R5965 T6944 T6941 punct ),clone
R5966 T6945 T6946 amod complete,cDNA
R5967 T6946 T6948 nsubjpass cDNA,obtained
R5968 T6947 T6948 auxpass was,obtained
R5969 T6948 T6948 ROOT obtained,obtained
R5970 T6949 T6948 prep from,obtained
R5971 T6950 T6952 compound Invitrogen,Technologies
R5972 T6951 T6952 compound Life,Technologies
R5973 T6952 T6949 pobj Technologies,from
R5974 T6953 T6948 prep as,obtained
R5975 T6954 T6962 det a,insert
R5976 T6955 T6958 nummod 5,EcoRI/3
R5977 T6956 T6958 punct ′,EcoRI/3
R5978 T6957 T6958 punct -,EcoRI/3
R5979 T6958 T6962 compound EcoRI/3,insert
R5980 T6959 T6961 punct ′,XhoI
R5981 T6960 T6961 punct -,XhoI
R5982 T6961 T6962 compound XhoI,insert
R5983 T6962 T6953 pobj insert,as
R5984 T6963 T6962 prep of,insert
R5985 T6964 T6963 pobj GATA-3,of
R5986 T6965 T6962 prep in,insert
R5987 T6966 T6968 det the,vector
R5988 T6967 T6968 compound pOTB7,vector
R5989 T6968 T6965 pobj vector,in
R5990 T6969 T6948 punct .,obtained
R5991 T6970 T6972 nsubjpass GATA-3,excised
R5992 T6971 T6972 auxpass was,excised
R5993 T6972 T6972 ROOT excised,excised
R5994 T6973 T6972 prep from,excised
R5995 T6974 T6973 pobj pOTB7,from
R5996 T6975 T6972 advcl using,excised
R5997 T6976 T6977 compound XhoI,digestion
R5998 T6977 T6975 dobj digestion,using
R5999 T6978 T6977 cc and,digestion
R6000 T6979 T6977 conj pEGFP-C2,digestion
R6001 T6980 T6981 punct (,Clontech
R6002 T6981 T6977 appos Clontech,digestion
R6003 T6982 T6981 punct ",",Clontech
R6004 T6983 T6981 conj Saint-Germain-en-Laye,Clontech
R6005 T6984 T6983 punct ",",Saint-Germain-en-Laye
R6006 T6985 T6983 conj France,Saint-Germain-en-Laye
R6007 T6986 T6981 punct ),Clontech
R6008 T6987 T6988 auxpass was,digested
R6009 T6988 T6972 advcl digested,excised
R6010 T6989 T6988 prep with,digested
R6011 T6990 T6989 pobj BamHI,with
R6012 T6991 T6972 punct .,excised
R6013 T6992 T6994 nsubjpass DNA,recovered
R6014 T6993 T6994 auxpass was,recovered
R6015 T6994 T6994 ROOT recovered,recovered
R6016 T6995 T6994 agent by,recovered
R6017 T6996 T6997 compound phenol,extraction
R6018 T6997 T6995 pobj extraction,by
R6019 T6998 T6997 cc and,extraction
R6020 T6999 T7000 compound ethanol,precipitation
R6021 T7000 T6997 conj precipitation,extraction
R6022 T7001 T6994 punct ",",recovered
R6023 T7002 T6994 cc and,recovered
R6024 T7003 T7006 preconj both,fragment
R6025 T7004 T7006 det the,fragment
R6026 T7005 T7006 compound GATA-3,fragment
R6027 T7006 T7029 nsubjpass fragment,inactivated
R6028 T7007 T7006 cc and,fragment
R6029 T7008 T7010 det the,vector
R6030 T7009 T7010 compound pEGFP-C2,vector
R6031 T7010 T7006 conj vector,fragment
R6032 T7011 T7006 acl blunt-ended,fragment
R6033 T7012 T7011 agent by,blunt-ended
R6034 T7013 T7012 pobj incubation,by
R6035 T7014 T7011 prep with,blunt-ended
R6036 T7015 T7014 pobj Klenow,with
R6037 T7016 T7015 punct (,Klenow
R6038 T7017 T7018 compound Bioline,Bio-27029
R6039 T7018 T7015 appos Bio-27029,Klenow
R6040 T7019 T7015 punct ),Klenow
R6041 T7020 T7011 prep for,blunt-ended
R6042 T7021 T7022 nummod 30,min
R6043 T7022 T7020 pobj min,for
R6044 T7023 T7022 prep at,min
R6045 T7024 T7025 nummod 37,°
R6046 T7025 T7027 nummod °,Klenow
R6047 T7026 T7027 compound C.,Klenow
R6048 T7027 T7023 pobj Klenow,at
R6049 T7028 T7029 auxpass was,inactivated
R6050 T7029 T6994 conj inactivated,recovered
R6051 T7030 T7029 agent by,inactivated
R6052 T7031 T7030 pobj incubation,by
R6053 T7032 T7029 prep for,inactivated
R6054 T7033 T7034 nummod 10,min
R6055 T7034 T7032 pobj min,for
R6056 T7035 T7034 prep at,min
R6057 T7036 T7037 nummod 75,°
R6058 T7037 T7039 nummod °,DNA
R6059 T7038 T7039 compound C.,DNA
R6060 T7039 T7035 pobj DNA,at
R6061 T7040 T7041 auxpass was,recovered
R6062 T7041 T6994 conj recovered,recovered
R6063 T7042 T7041 agent by,recovered
R6064 T7043 T7044 compound phenol,extraction
R6065 T7044 T7042 pobj extraction,by
R6066 T7045 T7044 cc and,extraction
R6067 T7046 T7047 compound ethanol,precipitation
R6068 T7047 T7044 conj precipitation,extraction
R6069 T7048 T7041 punct ",",recovered
R6070 T7049 T6994 cc and,recovered
R6071 T7050 T7054 preconj both,fragment
R6072 T7051 T7054 det the,fragment
R6073 T7052 T7054 amod blunt-ended,fragment
R6074 T7053 T7054 compound GATA-3,fragment
R6075 T7054 T7061 nsubjpass fragment,digested
R6076 T7055 T7054 cc and,fragment
R6077 T7056 T7058 det the,vector
R6078 T7057 T7058 compound GFP,vector
R6079 T7058 T7054 conj vector,fragment
R6080 T7059 T7061 auxpass were,digested
R6081 T7060 T7061 advmod subsequently,digested
R6082 T7061 T6994 conj digested,recovered
R6083 T7062 T7061 prep with,digested
R6084 T7063 T7062 pobj EcoRI,with
R6085 T7064 T7061 prep before,digested
R6086 T7065 T7070 det the,′
R6087 T7066 T7070 nummod 5,′
R6088 T7067 T7069 punct ′,EcoRI/3
R6089 T7068 T7069 punct -,EcoRI/3
R6090 T7069 T7070 compound EcoRI/3,′
R6091 T7070 T7064 pobj ′,before
R6092 T7071 T7074 amod blunt,fragment
R6093 T7072 T7074 compound end,fragment
R6094 T7073 T7074 compound GATA-3,fragment
R6095 T7074 T7076 nsubjpass fragment,inserted
R6096 T7075 T7076 auxpass was,inserted
R6097 T7076 T6994 conj inserted,recovered
R6098 T7077 T7076 prep into,inserted
R6099 T7078 T7083 det the,′
R6100 T7079 T7082 nummod 5,EcoRI/3
R6101 T7080 T7082 punct ′,EcoRI/3
R6102 T7081 T7082 punct -,EcoRI/3
R6103 T7082 T7083 compound EcoRI/3,′
R6104 T7083 T7077 pobj ′,into
R6105 T7084 T7085 nsubj blunt,ended
R6106 T7085 T7076 advcl ended,inserted
R6107 T7086 T7087 compound GFP,vector
R6108 T7087 T7085 dobj vector,ended
R6109 T7088 T7085 punct .,ended
R6110 T7089 T7090 amod Positive,clones
R6111 T7090 T7092 nsubjpass clones,confirmed
R6112 T7091 T7092 auxpass were,confirmed
R6113 T7092 T7092 ROOT confirmed,confirmed
R6114 T7093 T7092 agent by,confirmed
R6115 T7094 T7093 pobj digestion,by
R6116 T7095 T7094 cc and,digestion
R6117 T7096 T7094 conj size,digestion
R6118 T7097 T7094 conj analysis,digestion
R6119 T7098 T7092 prep by,confirmed
R6120 T7099 T7100 nummod 1,%
R6121 T7100 T7103 compound %,electrophoresis
R6122 T7101 T7102 amod agarose,gel
R6123 T7102 T7103 compound gel,electrophoresis
R6124 T7103 T7098 pobj electrophoresis,by
R6125 T7104 T7098 cc and,by
R6126 T7105 T7098 conj by,by
R6127 T7106 T7105 pcomp sequencing,by
R6128 T7107 T7092 punct .,confirmed

GO-BP

Id Subject Object Predicate Lexical cue
T7113 212-221 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion
T7114 861-870 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion

sentences

Id Subject Object Predicate Lexical cue
T6774 0-20 Sentence denotes GATA-3-GFP Construct
T6775 21-170 Sentence denotes The GATA-3 clone (BC003070) complete cDNA was obtained from Invitrogen Life Technologies as a 5′- EcoRI/3′-XhoI insert of GATA-3 in the pOTB7 vector.
T6776 171-301 Sentence denotes GATA-3 was excised from pOTB7 using XhoI digestion and pEGFP-C2 (Clontech, Saint-Germain-en-Laye, France) was digested with BamHI.
T6777 302-826 Sentence denotes DNA was recovered by phenol extraction and ethanol precipitation, and both the GATA-3 fragment and the pEGFP-C2 vector blunt-ended by incubation with Klenow (Bioline Bio-27029) for 30 min at 37°C. Klenow was inactivated by incubation for 10 min at 75°C. DNA was recovered by phenol extraction and ethanol precipitation, and both the blunt-ended GATA-3 fragment and the GFP vector were subsequently digested with EcoRI before the 5′-EcoRI/3′–blunt end GATA-3 fragment was inserted into the 5′-EcoRI/3′–blunt ended GFP vector.
T6778 827-941 Sentence denotes Positive clones were confirmed by digestion and size analysis by 1% agarose gel electrophoresis and by sequencing.
T140 0-20 Sentence denotes GATA-3-GFP Construct
T141 21-170 Sentence denotes The GATA-3 clone (BC003070) complete cDNA was obtained from Invitrogen Life Technologies as a 5′- EcoRI/3′-XhoI insert of GATA-3 in the pOTB7 vector.
T142 171-301 Sentence denotes GATA-3 was excised from pOTB7 using XhoI digestion and pEGFP-C2 (Clontech, Saint-Germain-en-Laye, France) was digested with BamHI.
T143 302-498 Sentence denotes DNA was recovered by phenol extraction and ethanol precipitation, and both the GATA-3 fragment and the pEGFP-C2 vector blunt-ended by incubation with Klenow (Bioline Bio-27029) for 30 min at 37°C.
T144 499-555 Sentence denotes Klenow was inactivated by incubation for 10 min at 75°C.
T145 556-826 Sentence denotes DNA was recovered by phenol extraction and ethanol precipitation, and both the blunt-ended GATA-3 fragment and the GFP vector were subsequently digested with EcoRI before the 5′-EcoRI/3′–blunt end GATA-3 fragment was inserted into the 5′-EcoRI/3′–blunt ended GFP vector.
T146 827-941 Sentence denotes Positive clones were confirmed by digestion and size analysis by 1% agarose gel electrophoresis and by sequencing.

events-check-again

Id Subject Object Predicate Lexical cue
T7155 0-6 Protein denotes GATA-3
T7156 7-10 Protein denotes GFP
T7157 25-31 Protein denotes GATA-3
T7158 143-149 Protein denotes GATA-3
T7159 171-177 Protein denotes GATA-3
T7160 207-211 Protein denotes XhoI
T7161 295-300 Protein denotes BamHI
T7162 381-387 Protein denotes GATA-3
T7163 647-653 Protein denotes GATA-3
T7164 671-674 Protein denotes GFP
T7165 714-719 Protein denotes EcoRI
T7166 753-759 Protein denotes GATA-3
T7167 815-818 Protein denotes GFP

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T7141 128-132 Protein denotes XhoI
T7142 143-149 Protein denotes GATA-3
T7143 157-169 Protein denotes pOTB7 vector
T7144 171-177 Protein denotes GATA-3
T7145 195-211 Protein denotes pOTB7 using XhoI
T7146 226-234 Protein denotes pEGFP-C2
T7147 295-300 Protein denotes BamHI
T7148 182-189 Negative_regulation denotes excised
T7149 182-189 Negative_regulation denotes excised
T7150 381-396 Protein denotes GATA-3 fragment
T7151 405-410 Protein denotes pEGFP
T7152 411-413 Protein denotes C2
T7153 714-719 Protein denotes EcoRI
T7154 753-768 Protein denotes GATA-3 fragment
R6129 T7144 T7148 themeOf GATA-3,excised
R6130 T7145 T7149 themeOf pOTB7 using XhoI,excised

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T6761 0-6 Protein denotes GATA-3
T6762 7-10 Protein denotes GFP
T6763 25-31 Protein denotes GATA-3
T6764 143-149 Protein denotes GATA-3
T6765 171-177 Protein denotes GATA-3
T6766 207-211 Protein denotes XhoI
T6767 295-300 Protein denotes BamHI
T6768 381-387 Protein denotes GATA-3
T6769 647-653 Protein denotes GATA-3
T6770 671-674 Protein denotes GFP
T6771 714-719 Protein denotes EcoRI
T6772 753-759 Protein denotes GATA-3
T6773 815-818 Protein denotes GFP

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T6927 0-6 P23771 denotes GATA-3
T6928 7-10 Q9U6Y4 denotes GFP
T6929 25-31 P23771 denotes GATA-3
T6930 143-149 P23771 denotes GATA-3
T6931 171-177 P23771 denotes GATA-3
T6932 381-387 P23771 denotes GATA-3
T6933 647-653 P23771 denotes GATA-3
T6934 671-674 Q9U6Y4 denotes GFP
T6935 753-759 P23771 denotes GATA-3
T6936 815-818 Q9U6Y4 denotes GFP

test2

Id Subject Object Predicate Lexical cue
T6747 0-6 Protein denotes GATA-3
T6748 7-10 Protein denotes GFP
T6749 25-31 Protein denotes GATA-3
T6750 143-149 Protein denotes GATA-3
T6751 171-177 Protein denotes GATA-3
T6752 182-189 Negative_regulation denotes excised
T6753 207-211 Protein denotes XhoI
T6754 295-300 Protein denotes BamHI
T6755 381-387 Protein denotes GATA-3
T6756 647-653 Protein denotes GATA-3
T6757 671-674 Protein denotes GFP
T6758 714-719 Protein denotes EcoRI
T6759 753-759 Protein denotes GATA-3
T6760 815-818 Protein denotes GFP
R5811 T6751 T6752 themeOf GATA-3,excised
R5812 T6756 T6758 themeOf GATA-3,EcoRI
R5813 T6757 T6758 themeOf GFP,EcoRI
R5814 T6759 T6758 themeOf GATA-3,EcoRI