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PMC:2674207 / 18514-19778 JSONTXT

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2_test

Id Subject Object Predicate Lexical cue
19436703-15860753-86274492 95-97 15860753 denotes 34
T87031 95-97 15860753 denotes 34

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T6283 0-18 NN denotes Immunofluorescence
T6284 19-27 NN denotes Staining
T6285 28-46 NN denotes Immunofluorescence
T6286 47-55 NN denotes staining
T6287 56-59 VB denotes was
T6288 60-69 VB denotes performed
T6289 70-72 IN denotes as
T6290 73-83 RB denotes previously
T6291 84-93 VB denotes described
T6292 94-95 -LRB- denotes [
T6293 95-97 CD denotes 34
T6294 97-98 -RRB- denotes ]
T6295 100-103 DT denotes All
T6296 104-112 NN denotes staining
T6297 113-116 VB denotes was
T6298 117-126 VB denotes performed
T6299 127-129 IN denotes at
T6300 130-134 NN denotes room
T6301 135-146 NN denotes temperature
T6302 147-150 CC denotes and
T6303 151-156 IN denotes under
T6304 157-171 NN denotes humidification
T6305 173-178 NN denotes Cells
T6306 179-183 VB denotes were
T6307 184-193 VB denotes collected
T6308 194-197 CC denotes and
T6309 198-207 NN denotes cytospins
T6310 208-216 VB denotes prepared
T6311 217-219 IN denotes in
T6312 220-221 DT denotes a
T6313 222-236 NN denotes cytocentrifuge
T6314 237-238 -LRB- denotes (
T6315 238-245 NNP denotes Shandon
T6316 246-248 NNP denotes II
T6317 248-249 -COMMA- denotes ,
T6318 250-257 NNP denotes Shandon
T6319 257-258 -COMMA- denotes ,
T6320 259-266 NNP denotes Runcorn
T6321 266-267 -COMMA- denotes ,
T6322 268-270 NNP denotes UK
T6323 270-271 -RRB- denotes )
T6324 273-278 NN denotes Cells
T6325 279-283 VB denotes were
T6326 284-297 VB denotes permeabilized
T6327 297-298 -COMMA- denotes ,
T6328 299-306 VB denotes blocked
T6329 306-307 -COMMA- denotes ,
T6330 308-311 CC denotes and
T6331 312-316 RB denotes then
T6332 317-326 VB denotes incubated
T6333 327-331 IN denotes with
T6334 332-334 NN denotes GR
T6335 335-336 -LRB- denotes (
T6336 336-340 CD denotes 1∶50
T6337 341-345 NN denotes E-20
T6338 346-351 NNP denotes Santa
T6339 352-356 NNP denotes Cruz
T6340 357-370 NNP denotes Biotechnology
T6341 370-371 -RRB- denotes )
T6342 372-374 CC denotes or
T6343 375-386 NN denotes anti-GATA-3
T6344 387-388 -LRB- denotes (
T6345 388-392 NN denotes H-48
T6346 392-393 -COLON- denotes ;
T6347 394-399 NNP denotes Santa
T6348 400-404 NNP denotes Cruz
T6349 405-418 NNP denotes Biotechnology
T6350 418-419 -RRB- denotes )
T6351 420-428 NN denotes antibody
T6352 429-432 IN denotes for
T6353 433-434 CD denotes 1
T6354 435-436 NN denotes h
T6355 437-439 IN denotes at
T6356 440-444 NN denotes room
T6357 445-456 NN denotes temperature
T6358 458-463 IN denotes After
T6359 464-469 CD denotes three
T6360 470-476 NN denotes washes
T6361 477-479 IN denotes in
T6362 480-498 JJ denotes phosphate-buffered
T6363 499-505 NN denotes saline
T6364 506-507 -LRB- denotes (
T6365 507-510 NN denotes PBS
T6366 510-511 -RRB- denotes )
T6367 511-512 -COMMA- denotes ,
T6368 513-518 NN denotes cells
T6369 519-523 VB denotes were
T6370 524-533 VB denotes incubated
T6371 534-538 IN denotes with
T6372 539-553 NN denotes tetrarhodamine
T6373 554-579 VB denotes isothiocyanate–conjugated
T6374 580-584 NN denotes goat
T6375 585-596 JJ denotes anti-rabbit
T6376 597-605 NN denotes antibody
T6377 606-607 -LRB- denotes (
T6378 607-611 NN denotes Dako
T6379 611-612 -RRB- denotes )
T6380 614-623 NN denotes Cytospins
T6381 624-628 VB denotes were
T6382 629-643 VB denotes counterstained
T6383 644-648 IN denotes with
T6384 649-651 NN denotes 4′
T6385 651-652 -COMMA- denotes ,
T6386 652-678 NN denotes 6-diamidino-2-phenylindole
T6387 679-694 NN denotes dihydrochloride
T6388 695-696 -LRB- denotes (
T6389 696-700 NN denotes DAPI
T6390 700-701 -RRB- denotes )
T6391 701-702 -COMMA- denotes ,
T6392 703-704 DT denotes a
T6393 705-716 JJ denotes fluorescent
T6394 717-721 JJ denotes blue
T6395 722-729 JJ denotes nuclear
T6396 730-736 NN denotes indole
T6397 737-746 NN denotes chromatin
T6398 747-752 NN denotes stain
T6399 752-753 -COMMA- denotes ,
T6400 754-757 CC denotes and
T6401 758-765 VB denotes mounted
T6402 766-768 IN denotes in
T6403 769-772 NNP denotes PBS
T6404 772-773 -COLON- denotes :
T6405 773-781 NN denotes glycerol
T6406 782-783 -LRB- denotes (
T6407 783-788 CD denotes 50∶50
T6408 788-789 -RRB- denotes )
T6409 791-794 DT denotes The
T6410 795-809 JJ denotes immunopositive
T6411 810-816 NN denotes signal
T6412 817-820 VB denotes was
T6413 821-834 VB denotes characterised
T6414 835-840 VB denotes using
T6415 841-846 NN denotes laser
T6416 847-855 NN denotes scanning
T6417 856-864 JJ denotes confocal
T6418 865-875 NN denotes microscopy
T6419 876-878 IN denotes on
T6420 879-880 DT denotes a
T6421 881-886 NNP denotes Leica
T6422 887-890 NNP denotes TCS
T6423 891-896 NNP denotes NT/SP
T6424 897-908 JJ denotes interactive
T6425 909-914 NN denotes laser
T6426 915-924 NN denotes cytometer
T6427 925-933 VB denotes equipped
T6428 934-938 IN denotes with
T6429 939-947 JJ denotes confocal
T6430 948-954 NN denotes optics
T6431 955-956 -LRB- denotes (
T6432 956-961 NNP denotes Leica
T6433 962-974 NNP denotes Microsystems
T6434 974-975 -COMMA- denotes ,
T6435 976-983 NNP denotes Wetzlar
T6436 983-984 -COMMA- denotes ,
T6437 985-992 NNP denotes Germany
T6438 992-993 -RRB- denotes )
T6439 995-997 TO denotes To
T6440 998-1007 VB denotes determine
T6441 1008-1011 DT denotes the
T6442 1012-1023 NN denotes specificity
T6443 1024-1026 IN denotes of
T6444 1027-1030 DT denotes the
T6445 1031-1041 NN denotes antibodies
T6446 1041-1042 -COMMA- denotes ,
T6447 1043-1049 NN denotes rabbit
T6448 1050-1055 NN denotes serum
T6449 1056-1070 NN denotes immunoglobulin
T6450 1071-1072 -LRB- denotes (
T6451 1072-1076 NN denotes Dako
T6452 1076-1077 -RRB- denotes )
T6453 1078-1081 CC denotes and
T6454 1082-1091 JJ denotes secondary
T6455 1092-1102 NN denotes antibodies
T6456 1103-1110 IN denotes without
T6457 1111-1114 DT denotes the
T6458 1115-1122 JJ denotes primary
T6459 1123-1127 VB denotes were
T6460 1128-1132 VB denotes used
T6461 1133-1135 IN denotes as
T6462 1136-1144 NN denotes controls
T6463 1146-1156 RB denotes Positively
T6464 1157-1164 VB denotes stained
T6465 1165-1171 NN denotes nuclei
T6466 1172-1175 CC denotes and
T6467 1176-1181 JJ denotes total
T6468 1182-1187 NN denotes cells
T6469 1188-1192 VB denotes were
T6470 1193-1200 VB denotes counted
T6471 1201-1202 -LRB- denotes (
T6472 1202-1205 CD denotes 500
T6473 1205-1206 -RRB- denotes )
T6474 1207-1209 IN denotes on
T6475 1210-1214 DT denotes each
T6476 1215-1220 NN denotes slide
T6477 1221-1225 IN denotes with
T6478 1226-1229 DT denotes the
T6479 1230-1238 NN denotes observer
T6480 1239-1246 VB denotes blinded
T6481 1247-1249 TO denotes to
T6482 1250-1253 DT denotes the
T6483 1254-1263 NN denotes treatment
R5389 T6284 T6283 arg1Of Staining,Immunofluorescence
R5390 T6286 T6285 arg1Of staining,Immunofluorescence
R5391 T6286 T6287 arg1Of staining,was
R5392 T6286 T6288 arg2Of staining,performed
R5393 T6288 T6287 arg2Of performed,was
R5394 T6288 T6289 arg1Of performed,as
R5395 T6291 T6290 arg1Of described,previously
R5396 T6293 T6289 arg2Of 34,as
R5397 T6293 T6291 arg1Of 34,described
R5398 T6293 T6292 arg2Of 34,[
R5399 T6294 T6292 arg3Of ],[
R5400 T6296 T6295 arg1Of staining,All
R5401 T6296 T6297 arg1Of staining,was
R5402 T6296 T6298 arg2Of staining,performed
R5403 T6298 T6297 arg2Of performed,was
R5404 T6298 T6299 arg1Of performed,at
R5405 T6298 T6303 arg1Of performed,under
R5406 T6299 T6302 arg1Of at,and
R5407 T6301 T6299 arg2Of temperature,at
R5408 T6301 T6300 arg1Of temperature,room
R5409 T6303 T6302 arg2Of under,and
R5410 T6304 T6303 arg2Of humidification,under
R5411 T6305 T6306 arg1Of Cells,were
R5412 T6305 T6307 arg2Of Cells,collected
R5413 T6307 T6306 arg2Of collected,were
R5414 T6307 T6308 arg1Of collected,and
R5415 T6308 T6314 arg1Of and,(
R5416 T6309 T6310 arg2Of cytospins,prepared
R5417 T6310 T6308 arg2Of prepared,and
R5418 T6310 T6311 arg1Of prepared,in
R5419 T6313 T6311 arg2Of cytocentrifuge,in
R5420 T6313 T6312 arg1Of cytocentrifuge,a
R5421 T6316 T6315 arg1Of II,Shandon
R5422 T6316 T6317 arg1Of II,","
R5423 T6317 T6319 arg1Of ",",","
R5424 T6318 T6317 arg2Of Shandon,","
R5425 T6319 T6314 arg2Of ",",(
R5426 T6319 T6321 arg1Of ",",","
R5427 T6320 T6319 arg2Of Runcorn,","
R5428 T6322 T6321 arg2Of UK,","
R5429 T6323 T6314 arg3Of ),(
R5430 T6324 T6325 arg1Of Cells,were
R5431 T6324 T6326 arg2Of Cells,permeabilized
R5432 T6324 T6328 arg2Of Cells,blocked
R5433 T6324 T6332 arg2Of Cells,incubated
R5434 T6326 T6327 arg1Of permeabilized,","
R5435 T6327 T6330 arg1Of ",",and
R5436 T6328 T6327 arg2Of blocked,","
R5437 T6330 T6325 arg2Of and,were
R5438 T6330 T6329 arg1Of and,","
R5439 T6332 T6330 arg2Of incubated,and
R5440 T6332 T6331 arg1Of incubated,then
R5441 T6332 T6333 arg1Of incubated,with
R5442 T6334 T6335 arg1Of GR,(
R5443 T6334 T6342 arg1Of GR,or
R5444 T6340 T6335 arg2Of Biotechnology,(
R5445 T6340 T6336 arg1Of Biotechnology,1∶50
R5446 T6340 T6337 arg1Of Biotechnology,E-20
R5447 T6340 T6338 arg1Of Biotechnology,Santa
R5448 T6340 T6339 arg1Of Biotechnology,Cruz
R5449 T6341 T6335 arg3Of ),(
R5450 T6342 T6333 arg2Of or,with
R5451 T6343 T6344 arg1Of anti-GATA-3,(
R5452 T6345 T6344 arg2Of H-48,(
R5453 T6345 T6346 arg1Of H-48,;
R5454 T6349 T6346 arg2Of Biotechnology,;
R5455 T6349 T6347 arg1Of Biotechnology,Santa
R5456 T6349 T6348 arg1Of Biotechnology,Cruz
R5457 T6350 T6344 arg3Of ),(
R5458 T6351 T6342 arg2Of antibody,or
R5459 T6351 T6343 arg1Of antibody,anti-GATA-3
R5460 T6351 T6352 arg1Of antibody,for
R5461 T6354 T6352 arg2Of h,for
R5462 T6354 T6353 arg1Of h,1
R5463 T6354 T6355 arg1Of h,at
R5464 T6357 T6355 arg2Of temperature,at
R5465 T6357 T6356 arg1Of temperature,room
R5466 T6360 T6358 arg2Of washes,After
R5467 T6360 T6359 arg1Of washes,three
R5468 T6360 T6361 arg1Of washes,in
R5469 T6363 T6361 arg2Of saline,in
R5470 T6363 T6362 arg1Of saline,phosphate-buffered
R5471 T6363 T6364 arg1Of saline,(
R5472 T6365 T6364 arg2Of PBS,(
R5473 T6366 T6364 arg3Of ),(
R5474 T6368 T6369 arg1Of cells,were
R5475 T6368 T6370 arg2Of cells,incubated
R5476 T6370 T6358 arg1Of incubated,After
R5477 T6370 T6367 arg1Of incubated,","
R5478 T6370 T6369 arg2Of incubated,were
R5479 T6370 T6371 arg1Of incubated,with
R5480 T6372 T6371 arg2Of tetrarhodamine,with
R5481 T6372 T6373 arg2Of tetrarhodamine,isothiocyanate–conjugated
R5482 T6376 T6373 arg3Of antibody,isothiocyanate–conjugated
R5483 T6376 T6374 arg1Of antibody,goat
R5484 T6376 T6375 arg1Of antibody,anti-rabbit
R5485 T6376 T6377 arg1Of antibody,(
R5486 T6378 T6377 arg2Of Dako,(
R5487 T6379 T6377 arg3Of ),(
R5488 T6380 T6381 arg1Of Cytospins,were
R5489 T6380 T6382 arg2Of Cytospins,counterstained
R5490 T6380 T6401 arg1Of Cytospins,mounted
R5491 T6382 T6381 arg2Of counterstained,were
R5492 T6382 T6383 arg1Of counterstained,with
R5493 T6382 T6400 arg1Of counterstained,and
R5494 T6384 T6383 arg2Of 4′,with
R5495 T6384 T6385 arg1Of 4′,","
R5496 T6384 T6391 arg1Of 4′,","
R5497 T6387 T6385 arg2Of dihydrochloride,","
R5498 T6387 T6386 arg1Of dihydrochloride,6-diamidino-2-phenylindole
R5499 T6387 T6388 arg1Of dihydrochloride,(
R5500 T6389 T6388 arg2Of DAPI,(
R5501 T6390 T6388 arg3Of ),(
R5502 T6398 T6391 arg2Of stain,","
R5503 T6398 T6392 arg1Of stain,a
R5504 T6398 T6393 arg1Of stain,fluorescent
R5505 T6398 T6394 arg1Of stain,blue
R5506 T6398 T6395 arg1Of stain,nuclear
R5507 T6398 T6396 arg1Of stain,indole
R5508 T6398 T6397 arg1Of stain,chromatin
R5509 T6400 T6399 arg1Of and,","
R5510 T6401 T6400 arg2Of mounted,and
R5511 T6401 T6402 arg1Of mounted,in
R5512 T6401 T6404 arg1Of mounted,:
R5513 T6403 T6402 arg2Of PBS,in
R5514 T6405 T6401 arg2Of glycerol,mounted
R5515 T6405 T6406 arg1Of glycerol,(
R5516 T6407 T6406 arg2Of 50∶50,(
R5517 T6408 T6406 arg3Of ),(
R5518 T6411 T6409 arg1Of signal,The
R5519 T6411 T6410 arg1Of signal,immunopositive
R5520 T6411 T6412 arg1Of signal,was
R5521 T6411 T6413 arg2Of signal,characterised
R5522 T6411 T6414 arg1Of signal,using
R5523 T6413 T6412 arg2Of characterised,was
R5524 T6414 T6413 arg3Of using,characterised
R5525 T6414 T6419 arg1Of using,on
R5526 T6418 T6414 arg2Of microscopy,using
R5527 T6418 T6415 arg1Of microscopy,laser
R5528 T6418 T6416 arg1Of microscopy,scanning
R5529 T6418 T6417 arg1Of microscopy,confocal
R5530 T6423 T6421 arg1Of NT/SP,Leica
R5531 T6423 T6422 arg1Of NT/SP,TCS
R5532 T6426 T6419 arg2Of cytometer,on
R5533 T6426 T6420 arg1Of cytometer,a
R5534 T6426 T6423 arg1Of cytometer,NT/SP
R5535 T6426 T6424 arg1Of cytometer,interactive
R5536 T6426 T6425 arg1Of cytometer,laser
R5537 T6426 T6427 arg2Of cytometer,equipped
R5538 T6427 T6428 arg1Of equipped,with
R5539 T6430 T6428 arg2Of optics,with
R5540 T6430 T6429 arg1Of optics,confocal
R5541 T6430 T6431 arg1Of optics,(
R5542 T6433 T6431 arg2Of Microsystems,(
R5543 T6433 T6432 arg1Of Microsystems,Leica
R5544 T6433 T6434 arg1Of Microsystems,","
R5545 T6435 T6434 arg2Of Wetzlar,","
R5546 T6435 T6436 arg1Of Wetzlar,","
R5547 T6437 T6436 arg2Of Germany,","
R5548 T6438 T6431 arg3Of ),(
R5549 T6440 T6439 arg1Of determine,To
R5550 T6442 T6440 arg2Of specificity,determine
R5551 T6442 T6441 arg1Of specificity,the
R5552 T6442 T6443 arg1Of specificity,of
R5553 T6445 T6443 arg2Of antibodies,of
R5554 T6445 T6444 arg1Of antibodies,the
R5555 T6449 T6447 arg1Of immunoglobulin,rabbit
R5556 T6449 T6448 arg1Of immunoglobulin,serum
R5557 T6449 T6450 arg1Of immunoglobulin,(
R5558 T6449 T6453 arg1Of immunoglobulin,and
R5559 T6451 T6450 arg2Of Dako,(
R5560 T6452 T6450 arg3Of ),(
R5561 T6453 T6456 arg1Of and,without
R5562 T6453 T6459 arg1Of and,were
R5563 T6453 T6460 arg2Of and,used
R5564 T6455 T6453 arg2Of antibodies,and
R5565 T6455 T6454 arg1Of antibodies,secondary
R5566 T6458 T6456 arg2Of primary,without
R5567 T6458 T6457 arg1Of primary,the
R5568 T6460 T6439 modOf used,To
R5569 T6460 T6446 arg1Of used,","
R5570 T6460 T6459 arg2Of used,were
R5571 T6460 T6461 arg1Of used,as
R5572 T6462 T6461 arg2Of controls,as
R5573 T6464 T6463 arg1Of stained,Positively
R5574 T6465 T6464 arg1Of nuclei,stained
R5575 T6465 T6466 arg1Of nuclei,and
R5576 T6466 T6469 arg1Of and,were
R5577 T6466 T6470 arg2Of and,counted
R5578 T6468 T6466 arg2Of cells,and
R5579 T6468 T6467 arg1Of cells,total
R5580 T6470 T6469 arg2Of counted,were
R5581 T6470 T6471 arg1Of counted,(
R5582 T6470 T6474 arg1Of counted,on
R5583 T6472 T6471 arg2Of 500,(
R5584 T6473 T6471 arg3Of ),(
R5585 T6476 T6474 arg2Of slide,on
R5586 T6476 T6475 arg1Of slide,each
R5587 T6476 T6477 arg1Of slide,with
R5588 T6479 T6477 arg2Of observer,with
R5589 T6479 T6478 arg1Of observer,the
R5590 T6479 T6480 arg2Of observer,blinded
R5591 T6480 T6481 arg1Of blinded,to
R5592 T6483 T6481 arg2Of treatment,to
R5593 T6483 T6482 arg1Of treatment,the

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T6486 0-18 NNP denotes Immunofluorescence
T6487 19-27 NNP denotes Staining
T6488 28-46 NNP denotes Immunofluorescence
T6489 47-55 VBG denotes staining
T6490 56-59 VBD denotes was
T6491 60-69 VBN denotes performed
T6492 70-72 IN denotes as
T6493 73-83 RB denotes previously
T6494 84-93 VBN denotes described
T6495 94-95 NNP denotes [
T6496 95-97 CD denotes 34
T6497 97-98 NNP denotes ]
T6498 98-99 . denotes .
T6499 100-103 DT denotes All
T6500 104-112 VBG denotes staining
T6501 113-116 VBD denotes was
T6502 117-126 VBN denotes performed
T6503 127-129 IN denotes at
T6504 130-134 NN denotes room
T6505 135-146 NN denotes temperature
T6506 147-150 CC denotes and
T6507 151-156 IN denotes under
T6508 157-171 NN denotes humidification
T6509 171-172 . denotes .
T6510 173-178 NNS denotes Cells
T6511 179-183 VBD denotes were
T6512 184-193 VBN denotes collected
T6513 194-197 CC denotes and
T6514 198-207 NNS denotes cytospins
T6515 208-216 VBN denotes prepared
T6516 217-219 IN denotes in
T6517 220-221 DT denotes a
T6518 222-236 NN denotes cytocentrifuge
T6519 237-238 -LRB- denotes (
T6520 238-245 NNP denotes Shandon
T6521 246-248 NNP denotes II
T6522 248-249 , denotes ,
T6523 250-257 NNP denotes Shandon
T6524 257-258 , denotes ,
T6525 259-266 NNP denotes Runcorn
T6526 266-267 , denotes ,
T6527 268-270 NNP denotes UK
T6528 270-271 -RRB- denotes )
T6529 271-272 . denotes .
T6530 273-278 NNS denotes Cells
T6531 279-283 VBD denotes were
T6532 284-297 VBN denotes permeabilized
T6533 297-298 , denotes ,
T6534 299-306 VBN denotes blocked
T6535 306-307 , denotes ,
T6536 308-311 CC denotes and
T6537 312-316 RB denotes then
T6538 317-326 VBN denotes incubated
T6539 327-331 IN denotes with
T6540 332-334 NNP denotes GR
T6541 335-336 -LRB- denotes (
T6542 336-337 CD denotes 1
T6543 337-338 CD denotes ∶
T6544 338-340 CD denotes 50
T6545 341-345 NN denotes E-20
T6546 346-351 NNP denotes Santa
T6547 352-356 NNP denotes Cruz
T6548 357-370 NNP denotes Biotechnology
T6549 370-371 -RRB- denotes )
T6550 372-374 CC denotes or
T6551 375-386 JJ denotes anti-GATA-3
T6552 387-388 -LRB- denotes (
T6553 388-392 JJ denotes H-48
T6554 392-393 : denotes ;
T6555 394-399 NNP denotes Santa
T6556 400-404 NNP denotes Cruz
T6557 405-418 NNP denotes Biotechnology
T6558 418-419 -RRB- denotes )
T6559 420-428 NN denotes antibody
T6560 429-432 IN denotes for
T6561 433-434 CD denotes 1
T6562 435-436 NN denotes h
T6563 437-439 IN denotes at
T6564 440-444 NN denotes room
T6565 445-456 NN denotes temperature
T6566 456-457 . denotes .
T6567 458-463 IN denotes After
T6568 464-469 CD denotes three
T6569 470-476 NNS denotes washes
T6570 477-479 IN denotes in
T6571 480-498 JJ denotes phosphate-buffered
T6572 499-505 NN denotes saline
T6573 506-507 -LRB- denotes (
T6574 507-510 NNP denotes PBS
T6575 510-511 -RRB- denotes )
T6576 511-512 , denotes ,
T6577 513-518 NNS denotes cells
T6578 519-523 VBD denotes were
T6579 524-533 VBN denotes incubated
T6580 534-538 IN denotes with
T6581 539-553 JJ denotes tetrarhodamine
T6582 554-568 NN denotes isothiocyanate
T6583 569-579 VBN denotes conjugated
T6584 580-584 NN denotes goat
T6585 585-596 JJ denotes anti-rabbit
T6586 597-605 NN denotes antibody
T6587 606-607 -LRB- denotes (
T6588 607-611 NNP denotes Dako
T6589 611-612 -RRB- denotes )
T6590 612-613 . denotes .
T6591 614-623 NNS denotes Cytospins
T6592 624-628 VBD denotes were
T6593 629-643 VBN denotes counterstained
T6594 644-648 IN denotes with
T6595 649-650 CD denotes 4
T6596 650-651 CD denotes ′
T6597 651-653 SYM denotes ,6
T6598 653-654 : denotes -
T6599 654-678 JJ denotes diamidino-2-phenylindole
T6600 679-694 NN denotes dihydrochloride
T6601 695-696 -LRB- denotes (
T6602 696-700 NNP denotes DAPI
T6603 700-701 -RRB- denotes )
T6604 701-702 , denotes ,
T6605 703-704 DT denotes a
T6606 705-716 JJ denotes fluorescent
T6607 717-721 JJ denotes blue
T6608 722-729 JJ denotes nuclear
T6609 730-736 NN denotes indole
T6610 737-746 NN denotes chromatin
T6611 747-752 VBP denotes stain
T6612 752-753 , denotes ,
T6613 754-757 CC denotes and
T6614 758-765 VBD denotes mounted
T6615 766-768 IN denotes in
T6616 769-772 NNP denotes PBS
T6617 772-773 : denotes :
T6618 773-781 NN denotes glycerol
T6619 782-783 -LRB- denotes (
T6620 783-785 CD denotes 50
T6621 785-786 NN denotes ∶
T6622 786-788 CD denotes 50
T6623 788-789 -RRB- denotes )
T6624 789-790 . denotes .
T6625 791-794 DT denotes The
T6626 795-809 JJ denotes immunopositive
T6627 810-816 NN denotes signal
T6628 817-820 VBD denotes was
T6629 821-834 VBN denotes characterised
T6630 835-840 VBG denotes using
T6631 841-846 NN denotes laser
T6632 847-855 VBG denotes scanning
T6633 856-864 JJ denotes confocal
T6634 865-875 NN denotes microscopy
T6635 876-878 IN denotes on
T6636 879-880 DT denotes a
T6637 881-886 NNP denotes Leica
T6638 887-890 NNP denotes TCS
T6639 891-896 NNP denotes NT/SP
T6640 897-908 JJ denotes interactive
T6641 909-914 NN denotes laser
T6642 915-924 NN denotes cytometer
T6643 925-933 VBN denotes equipped
T6644 934-938 IN denotes with
T6645 939-947 JJ denotes confocal
T6646 948-954 NNS denotes optics
T6647 955-956 -LRB- denotes (
T6648 956-961 NNP denotes Leica
T6649 962-974 NNP denotes Microsystems
T6650 974-975 , denotes ,
T6651 976-983 NNP denotes Wetzlar
T6652 983-984 , denotes ,
T6653 985-992 NNP denotes Germany
T6654 992-993 -RRB- denotes )
T6655 993-994 . denotes .
T6656 995-997 TO denotes To
T6657 998-1007 VB denotes determine
T6658 1008-1011 DT denotes the
T6659 1012-1023 NN denotes specificity
T6660 1024-1026 IN denotes of
T6661 1027-1030 DT denotes the
T6662 1031-1041 NNS denotes antibodies
T6663 1041-1042 , denotes ,
T6664 1043-1049 NN denotes rabbit
T6665 1050-1055 NN denotes serum
T6666 1056-1070 NN denotes immunoglobulin
T6667 1071-1072 -LRB- denotes (
T6668 1072-1076 NNP denotes Dako
T6669 1076-1077 -RRB- denotes )
T6670 1078-1081 CC denotes and
T6671 1082-1091 JJ denotes secondary
T6672 1092-1102 NNS denotes antibodies
T6673 1103-1110 IN denotes without
T6674 1111-1114 DT denotes the
T6675 1115-1122 JJ denotes primary
T6676 1123-1127 VBD denotes were
T6677 1128-1132 VBN denotes used
T6678 1133-1135 IN denotes as
T6679 1136-1144 NNS denotes controls
T6680 1144-1145 . denotes .
T6681 1146-1156 RB denotes Positively
T6682 1157-1164 VBN denotes stained
T6683 1165-1171 NNS denotes nuclei
T6684 1172-1175 CC denotes and
T6685 1176-1181 JJ denotes total
T6686 1182-1187 NNS denotes cells
T6687 1188-1192 VBD denotes were
T6688 1193-1200 VBN denotes counted
T6689 1201-1202 -LRB- denotes (
T6690 1202-1205 CD denotes 500
T6691 1205-1206 -RRB- denotes )
T6692 1207-1209 IN denotes on
T6693 1210-1214 DT denotes each
T6694 1215-1220 NN denotes slide
T6695 1221-1225 IN denotes with
T6696 1226-1229 DT denotes the
T6697 1230-1238 NN denotes observer
T6698 1239-1246 VBD denotes blinded
T6699 1247-1249 TO denotes to
T6700 1250-1253 DT denotes the
T6701 1254-1263 NN denotes treatment
T6702 1263-1264 . denotes .
R5594 T6486 T6488 compound Immunofluorescence,Immunofluorescence
R5595 T6487 T6488 compound Staining,Immunofluorescence
R5596 T6488 T6491 nsubjpass Immunofluorescence,performed
R5597 T6489 T6488 acl staining,Immunofluorescence
R5598 T6490 T6491 auxpass was,performed
R5599 T6491 T6491 ROOT performed,performed
R5600 T6492 T6494 mark as,described
R5601 T6493 T6494 advmod previously,described
R5602 T6494 T6491 advcl described,performed
R5603 T6495 T6497 nmod [,]
R5604 T6496 T6497 nummod 34,]
R5605 T6497 T6494 dobj ],described
R5606 T6498 T6491 punct .,performed
R5607 T6499 T6500 det All,staining
R5608 T6500 T6502 nsubjpass staining,performed
R5609 T6501 T6502 auxpass was,performed
R5610 T6502 T6502 ROOT performed,performed
R5611 T6503 T6502 prep at,performed
R5612 T6504 T6505 compound room,temperature
R5613 T6505 T6503 pobj temperature,at
R5614 T6506 T6503 cc and,at
R5615 T6507 T6503 conj under,at
R5616 T6508 T6507 pobj humidification,under
R5617 T6509 T6502 punct .,performed
R5618 T6510 T6512 nsubjpass Cells,collected
R5619 T6511 T6512 auxpass were,collected
R5620 T6512 T6512 ROOT collected,collected
R5621 T6513 T6512 cc and,collected
R5622 T6514 T6512 conj cytospins,collected
R5623 T6515 T6514 acl prepared,cytospins
R5624 T6516 T6515 prep in,prepared
R5625 T6517 T6518 det a,cytocentrifuge
R5626 T6518 T6516 pobj cytocentrifuge,in
R5627 T6519 T6521 punct (,II
R5628 T6520 T6521 compound Shandon,II
R5629 T6521 T6518 appos II,cytocentrifuge
R5630 T6522 T6521 punct ",",II
R5631 T6523 T6521 conj Shandon,II
R5632 T6524 T6523 punct ",",Shandon
R5633 T6525 T6523 conj Runcorn,Shandon
R5634 T6526 T6525 punct ",",Runcorn
R5635 T6527 T6525 conj UK,Runcorn
R5636 T6528 T6521 punct ),II
R5637 T6529 T6512 punct .,collected
R5638 T6530 T6532 nsubjpass Cells,permeabilized
R5639 T6531 T6532 auxpass were,permeabilized
R5640 T6532 T6532 ROOT permeabilized,permeabilized
R5641 T6533 T6532 punct ",",permeabilized
R5642 T6534 T6532 conj blocked,permeabilized
R5643 T6535 T6534 punct ",",blocked
R5644 T6536 T6532 cc and,permeabilized
R5645 T6537 T6538 advmod then,incubated
R5646 T6538 T6532 conj incubated,permeabilized
R5647 T6539 T6538 prep with,incubated
R5648 T6540 T6539 pobj GR,with
R5649 T6541 T6542 punct (,1
R5650 T6542 T6548 nummod 1,Biotechnology
R5651 T6543 T6548 nummod ∶,Biotechnology
R5652 T6544 T6545 nummod 50,E-20
R5653 T6545 T6548 compound E-20,Biotechnology
R5654 T6546 T6547 compound Santa,Cruz
R5655 T6547 T6548 compound Cruz,Biotechnology
R5656 T6548 T6540 appos Biotechnology,GR
R5657 T6549 T6538 punct ),incubated
R5658 T6550 T6538 cc or,incubated
R5659 T6551 T6538 conj anti-GATA-3,incubated
R5660 T6552 T6559 punct (,antibody
R5661 T6553 T6559 amod H-48,antibody
R5662 T6554 T6559 punct ;,antibody
R5663 T6555 T6556 compound Santa,Cruz
R5664 T6556 T6557 compound Cruz,Biotechnology
R5665 T6557 T6559 compound Biotechnology,antibody
R5666 T6558 T6557 punct ),Biotechnology
R5667 T6559 T6559 ROOT antibody,antibody
R5668 T6560 T6559 prep for,antibody
R5669 T6561 T6562 nummod 1,h
R5670 T6562 T6560 pobj h,for
R5671 T6563 T6562 prep at,h
R5672 T6564 T6565 compound room,temperature
R5673 T6565 T6563 pobj temperature,at
R5674 T6566 T6532 punct .,permeabilized
R5675 T6567 T6579 prep After,incubated
R5676 T6568 T6569 nummod three,washes
R5677 T6569 T6567 pobj washes,After
R5678 T6570 T6569 prep in,washes
R5679 T6571 T6572 amod phosphate-buffered,saline
R5680 T6572 T6570 pobj saline,in
R5681 T6573 T6574 punct (,PBS
R5682 T6574 T6569 appos PBS,washes
R5683 T6575 T6569 punct ),washes
R5684 T6576 T6579 punct ",",incubated
R5685 T6577 T6579 nsubjpass cells,incubated
R5686 T6578 T6579 auxpass were,incubated
R5687 T6579 T6579 ROOT incubated,incubated
R5688 T6580 T6579 prep with,incubated
R5689 T6581 T6582 amod tetrarhodamine,isothiocyanate
R5690 T6582 T6580 pobj isothiocyanate,with
R5691 T6583 T6586 amod conjugated,antibody
R5692 T6584 T6586 nmod goat,antibody
R5693 T6585 T6586 amod anti-rabbit,antibody
R5694 T6586 T6586 ROOT antibody,antibody
R5695 T6587 T6588 punct (,Dako
R5696 T6588 T6586 appos Dako,antibody
R5697 T6589 T6588 punct ),Dako
R5698 T6590 T6586 punct .,antibody
R5699 T6591 T6593 nsubjpass Cytospins,counterstained
R5700 T6592 T6593 auxpass were,counterstained
R5701 T6593 T6593 ROOT counterstained,counterstained
R5702 T6594 T6593 prep with,counterstained
R5703 T6595 T6596 nummod 4,′
R5704 T6596 T6600 nummod ′,dihydrochloride
R5705 T6597 T6599 punct ",6",diamidino-2-phenylindole
R5706 T6598 T6599 punct -,diamidino-2-phenylindole
R5707 T6599 T6600 compound diamidino-2-phenylindole,dihydrochloride
R5708 T6600 T6594 pobj dihydrochloride,with
R5709 T6601 T6600 punct (,dihydrochloride
R5710 T6602 T6600 appos DAPI,dihydrochloride
R5711 T6603 T6600 punct ),dihydrochloride
R5712 T6604 T6600 punct ",",dihydrochloride
R5713 T6605 T6611 det a,stain
R5714 T6606 T6611 amod fluorescent,stain
R5715 T6607 T6609 amod blue,indole
R5716 T6608 T6609 amod nuclear,indole
R5717 T6609 T6611 compound indole,stain
R5718 T6610 T6611 compound chromatin,stain
R5719 T6611 T6600 appos stain,dihydrochloride
R5720 T6612 T6593 punct ",",counterstained
R5721 T6613 T6593 cc and,counterstained
R5722 T6614 T6593 conj mounted,counterstained
R5723 T6615 T6614 prep in,mounted
R5724 T6616 T6615 pobj PBS,in
R5725 T6617 T6614 punct :,mounted
R5726 T6618 T6614 conj glycerol,mounted
R5727 T6619 T6618 punct (,glycerol
R5728 T6620 T6621 nummod 50,∶
R5729 T6621 T6618 appos ∶,glycerol
R5730 T6622 T6621 nummod 50,∶
R5731 T6623 T6618 punct ),glycerol
R5732 T6624 T6593 punct .,counterstained
R5733 T6625 T6627 det The,signal
R5734 T6626 T6627 amod immunopositive,signal
R5735 T6627 T6629 nsubjpass signal,characterised
R5736 T6628 T6629 auxpass was,characterised
R5737 T6629 T6629 ROOT characterised,characterised
R5738 T6630 T6629 advcl using,characterised
R5739 T6631 T6632 nmod laser,scanning
R5740 T6632 T6630 dobj scanning,using
R5741 T6633 T6634 amod confocal,microscopy
R5742 T6634 T6632 dobj microscopy,scanning
R5743 T6635 T6632 prep on,scanning
R5744 T6636 T6642 det a,cytometer
R5745 T6637 T6639 nmod Leica,NT/SP
R5746 T6638 T6639 compound TCS,NT/SP
R5747 T6639 T6642 nmod NT/SP,cytometer
R5748 T6640 T6642 amod interactive,cytometer
R5749 T6641 T6642 compound laser,cytometer
R5750 T6642 T6635 pobj cytometer,on
R5751 T6643 T6642 acl equipped,cytometer
R5752 T6644 T6643 prep with,equipped
R5753 T6645 T6646 compound confocal,optics
R5754 T6646 T6644 pobj optics,with
R5782 T6674 T6675 det the,primary
R5783 T6675 T6673 pobj primary,without
R5784 T6676 T6677 auxpass were,used
R5785 T6677 T6677 ROOT used,used
R5786 T6678 T6677 prep as,used
R5787 T6679 T6678 pobj controls,as
R5788 T6680 T6677 punct .,used
R5789 T6681 T6682 advmod Positively,stained
R5790 T6682 T6683 amod stained,nuclei
R5791 T6683 T6688 nsubjpass nuclei,counted
R5792 T6684 T6683 cc and,nuclei
R5793 T6685 T6686 amod total,cells
R5794 T6686 T6683 conj cells,nuclei
R5795 T6687 T6688 auxpass were,counted
R5796 T6688 T6688 ROOT counted,counted
R5797 T6689 T6688 punct (,counted
R5798 T6690 T6688 npadvmod 500,counted
R5799 T6691 T6688 punct ),counted
R5800 T6692 T6688 prep on,counted
R5801 T6693 T6694 det each,slide
R5802 T6694 T6692 pobj slide,on
R5803 T6695 T6694 prep with,slide
R5804 T6696 T6697 det the,observer
R5805 T6697 T6695 pobj observer,with
R5806 T6698 T6688 conj blinded,counted
R5807 T6699 T6698 prep to,blinded
R5808 T6700 T6701 det the,treatment
R5809 T6701 T6699 pobj treatment,to
R5810 T6702 T6688 punct .,counted
R5755 T6647 T6646 punct (,optics
R5756 T6648 T6649 compound Leica,Microsystems
R5757 T6649 T6646 appos Microsystems,optics
R5758 T6650 T6649 punct ",",Microsystems
R5759 T6651 T6649 conj Wetzlar,Microsystems
R5760 T6652 T6651 punct ",",Wetzlar
R5761 T6653 T6651 conj Germany,Wetzlar
R5762 T6654 T6646 punct ),optics
R5763 T6655 T6629 punct .,characterised
R5764 T6656 T6657 aux To,determine
R5765 T6657 T6677 advcl determine,used
R5766 T6658 T6659 det the,specificity
R5767 T6659 T6657 dobj specificity,determine
R5768 T6660 T6659 prep of,specificity
R5769 T6661 T6662 det the,antibodies
R5770 T6662 T6660 pobj antibodies,of
R5771 T6663 T6662 punct ",",antibodies
R5772 T6664 T6665 compound rabbit,serum
R5773 T6665 T6666 compound serum,immunoglobulin
R5774 T6666 T6659 conj immunoglobulin,specificity
R5775 T6667 T6666 punct (,immunoglobulin
R5776 T6668 T6666 appos Dako,immunoglobulin
R5777 T6669 T6666 punct ),immunoglobulin
R5778 T6670 T6666 cc and,immunoglobulin
R5779 T6671 T6672 amod secondary,antibodies
R5780 T6672 T6666 conj antibodies,immunoglobulin
R5781 T6673 T6657 prep without,determine

UBERON-AE

Id Subject Object Predicate Lexical cue
T6271 1050-1055 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T6713 810-816 http://purl.obolibrary.org/obo/GO_0023052 denotes signal

GO-MF

Id Subject Object Predicate Lexical cue
T6714 420-428 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6715 597-605 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6716 1031-1041 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T6717 1056-1070 http://purl.obolibrary.org/obo/GO_0003823 denotes immunoglobulin

GO-CC

Id Subject Object Predicate Lexical cue
T6718 420-428 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6719 597-605 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6720 1031-1041 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T6721 1092-1102 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T6722 420-428 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6723 597-605 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6724 1031-1041 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T6725 1092-1102 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T6726 513-518 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6727 1182-1187 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6728 737-746 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin

sentences

Id Subject Object Predicate Lexical cue
T6273 0-27 Sentence denotes Immunofluorescence Staining
T6274 28-99 Sentence denotes Immunofluorescence staining was performed as previously described [34].
T6275 100-172 Sentence denotes All staining was performed at room temperature and under humidification.
T6276 173-272 Sentence denotes Cells were collected and cytospins prepared in a cytocentrifuge (Shandon II, Shandon, Runcorn, UK).
T6277 273-457 Sentence denotes Cells were permeabilized, blocked, and then incubated with GR (1∶50 E-20 Santa Cruz Biotechnology) or anti-GATA-3 (H-48; Santa Cruz Biotechnology) antibody for 1 h at room temperature.
T6278 458-613 Sentence denotes After three washes in phosphate-buffered saline (PBS), cells were incubated with tetrarhodamine isothiocyanate–conjugated goat anti-rabbit antibody (Dako).
T6279 614-790 Sentence denotes Cytospins were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI), a fluorescent blue nuclear indole chromatin stain, and mounted in PBS:glycerol (50∶50).
T6280 791-994 Sentence denotes The immunopositive signal was characterised using laser scanning confocal microscopy on a Leica TCS NT/SP interactive laser cytometer equipped with confocal optics (Leica Microsystems, Wetzlar, Germany).
T6281 995-1145 Sentence denotes To determine the specificity of the antibodies, rabbit serum immunoglobulin (Dako) and secondary antibodies without the primary were used as controls.
T6282 1146-1264 Sentence denotes Positively stained nuclei and total cells were counted (500) on each slide with the observer blinded to the treatment.
T130 0-27 Sentence denotes Immunofluorescence Staining
T131 28-99 Sentence denotes Immunofluorescence staining was performed as previously described [34].
T132 100-172 Sentence denotes All staining was performed at room temperature and under humidification.
T133 173-272 Sentence denotes Cells were collected and cytospins prepared in a cytocentrifuge (Shandon II, Shandon, Runcorn, UK).
T134 273-457 Sentence denotes Cells were permeabilized, blocked, and then incubated with GR (1∶50 E-20 Santa Cruz Biotechnology) or anti-GATA-3 (H-48; Santa Cruz Biotechnology) antibody for 1 h at room temperature.
T135 458-613 Sentence denotes After three washes in phosphate-buffered saline (PBS), cells were incubated with tetrarhodamine isothiocyanate–conjugated goat anti-rabbit antibody (Dako).
T136 614-790 Sentence denotes Cytospins were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI), a fluorescent blue nuclear indole chromatin stain, and mounted in PBS:glycerol (50∶50).
T137 791-994 Sentence denotes The immunopositive signal was characterised using laser scanning confocal microscopy on a Leica TCS NT/SP interactive laser cytometer equipped with confocal optics (Leica Microsystems, Wetzlar, Germany).
T138 995-1145 Sentence denotes To determine the specificity of the antibodies, rabbit serum immunoglobulin (Dako) and secondary antibodies without the primary were used as controls.
T139 1146-1264 Sentence denotes Positively stained nuclei and total cells were counted (500) on each slide with the observer blinded to the treatment.

events-check-again

Id Subject Object Predicate Lexical cue
T6731 332-334 Protein denotes GR

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T6732 1050-1070 Protein denotes serum immunoglobulin
T6733 1072-1076 Protein denotes Dako

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T6272 332-334 Protein denotes GR

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T6484 332-334 P04150 denotes GR
T6485 380-386 P23771 denotes GATA-3

test2

Id Subject Object Predicate Lexical cue
T6270 332-334 Protein denotes GR