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In Vitro Competition Assay for Importin-α (Far-Western ELISA)\nImmunoprecipitated importin-α (anti-importin-α, Santa Cruz Biotechnology) from HuT-78 cells was separated by SDS-PAGE and purified from the excised gel by electroelution [36]. Similarly, GATA-3 was isolated from nonstimulated HuT-78 cells or cells stimulated for 30 min with anti-CD3/CD28 and GR was isolated from FP (10−8 M, 30 min) stimulated HuT-78 cells. These proteins were subsequently refolded in glycine solution. 100 µl of importin-α solution (100 ng/ml in TBS) was added to 96-well plates coated with goat anti-importin-α antibody. After 1 h incubation, GATA-3 (100 ng/ml in TBS) from stimulated or unstimulated cells were added to the importin-α-coated wells. GR (10 or 100 ng/ml in TBS) from stimulated or unstimulated cells were added to some wells. After a further 1 h incubation, the plate was washed and incubated with primary antibodies (a mixture of rabbit anti-GATA-3 and mouse anti-GR, Santa Cruz Biotechnology) for 1.5 h, and then incubated with secondary antibodies. FITC swine anti-rabbit IgG (Dako, Cambridge, UK) was used for the detection of GATA-3, and rhodamine donkey anti-mouse IgG (Novus, Littleton, Colorado, USA) was used for the detection of GR. FITC and rhodamine levels were measured with a fluorescent micro-plate reader (Bioline, London, UK)."}

{"project":"GO-MF","denotations":[{"id":"T6017","span":{"begin":604,"end":612},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T6018","span":{"begin":915,"end":925},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T6019","span":{"begin":1049,"end":1059},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"GR-GATA-3 In Vitro Competition Assay for Importin-α (Far-Western ELISA)\nImmunoprecipitated importin-α (anti-importin-α, Santa Cruz Biotechnology) from HuT-78 cells was separated by SDS-PAGE and purified from the excised gel by electroelution [36]. Similarly, GATA-3 was isolated from nonstimulated HuT-78 cells or cells stimulated for 30 min with anti-CD3/CD28 and GR was isolated from FP (10−8 M, 30 min) stimulated HuT-78 cells. These proteins were subsequently refolded in glycine solution. 100 µl of importin-α solution (100 ng/ml in TBS) was added to 96-well plates coated with goat anti-importin-α antibody. After 1 h incubation, GATA-3 (100 ng/ml in TBS) from stimulated or unstimulated cells were added to the importin-α-coated wells. GR (10 or 100 ng/ml in TBS) from stimulated or unstimulated cells were added to some wells. After a further 1 h incubation, the plate was washed and incubated with primary antibodies (a mixture of rabbit anti-GATA-3 and mouse anti-GR, Santa Cruz Biotechnology) for 1.5 h, and then incubated with secondary antibodies. FITC swine anti-rabbit IgG (Dako, Cambridge, UK) was used for the detection of GATA-3, and rhodamine donkey anti-mouse IgG (Novus, Littleton, Colorado, USA) was used for the detection of GR. FITC and rhodamine levels were measured with a fluorescent micro-plate reader (Bioline, London, UK)."}

{"project":"GO-CC","denotations":[{"id":"T6020","span":{"begin":158,"end":163},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T6021","span":{"begin":305,"end":310},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T6022","span":{"begin":314,"end":319},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T6023","span":{"begin":424,"end":429},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T6024","span":{"begin":694,"end":699},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T6025","span":{"begin":803,"end":808},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T6026","span":{"begin":604,"end":612},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T6027","span":{"begin":915,"end":925},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T6028","span":{"begin":1049,"end":1059},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T6029","span":{"begin":604,"end":612},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T6030","span":{"begin":915,"end":925},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T6031","span":{"begin":1049,"end":1059},"obj":"http://purl.obolibrary.org/obo/GO_0042571"}],"text":"GR-GATA-3 In Vitro Competition Assay for Importin-α (Far-Western ELISA)\nImmunoprecipitated importin-α (anti-importin-α, Santa Cruz Biotechnology) from HuT-78 cells was separated by SDS-PAGE and purified from the excised gel by electroelution [36]. Similarly, GATA-3 was isolated from nonstimulated HuT-78 cells or cells stimulated for 30 min with anti-CD3/CD28 and GR was isolated from FP (10−8 M, 30 min) stimulated HuT-78 cells. These proteins were subsequently refolded in glycine solution. 100 µl of importin-α solution (100 ng/ml in TBS) was added to 96-well plates coated with goat anti-importin-α antibody. After 1 h incubation, GATA-3 (100 ng/ml in TBS) from stimulated or unstimulated cells were added to the importin-α-coated wells. GR (10 or 100 ng/ml in TBS) from stimulated or unstimulated cells were added to some wells. After a further 1 h incubation, the plate was washed and incubated with primary antibodies (a mixture of rabbit anti-GATA-3 and mouse anti-GR, Santa Cruz Biotechnology) for 1.5 h, and then incubated with secondary antibodies. FITC swine anti-rabbit IgG (Dako, Cambridge, UK) was used for the detection of GATA-3, and rhodamine donkey anti-mouse IgG (Novus, Littleton, Colorado, USA) was used for the detection of GR. FITC and rhodamine levels were measured with a fluorescent micro-plate reader (Bioline, London, UK)."}

{"project":"sentences","denotations":[{"id":"T5489","span":{"begin":0,"end":71},"obj":"Sentence"},{"id":"T5490","span":{"begin":72,"end":247},"obj":"Sentence"},{"id":"T5491","span":{"begin":248,"end":430},"obj":"Sentence"},{"id":"T5492","span":{"begin":431,"end":493},"obj":"Sentence"},{"id":"T5493","span":{"begin":494,"end":613},"obj":"Sentence"},{"id":"T5494","span":{"begin":614,"end":742},"obj":"Sentence"},{"id":"T5495","span":{"begin":743,"end":834},"obj":"Sentence"},{"id":"T5496","span":{"begin":835,"end":1060},"obj":"Sentence"},{"id":"T5497","span":{"begin":1061,"end":1251},"obj":"Sentence"},{"id":"T5498","span":{"begin":1252,"end":1352},"obj":"Sentence"},{"id":"T114","span":{"begin":0,"end":71},"obj":"Sentence"},{"id":"T115","span":{"begin":72,"end":247},"obj":"Sentence"},{"id":"T116","span":{"begin":248,"end":430},"obj":"Sentence"},{"id":"T117","span":{"begin":431,"end":493},"obj":"Sentence"},{"id":"T118","span":{"begin":494,"end":613},"obj":"Sentence"},{"id":"T119","span":{"begin":614,"end":742},"obj":"Sentence"},{"id":"T120","span":{"begin":743,"end":834},"obj":"Sentence"},{"id":"T121","span":{"begin":835,"end":1060},"obj":"Sentence"},{"id":"T122","span":{"begin":1061,"end":1251},"obj":"Sentence"},{"id":"T123","span":{"begin":1252,"end":1352},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"GR-GATA-3 In Vitro Competition Assay for Importin-α (Far-Western ELISA)\nImmunoprecipitated importin-α (anti-importin-α, Santa Cruz Biotechnology) from HuT-78 cells was separated by SDS-PAGE and purified from the excised gel by electroelution [36]. Similarly, GATA-3 was isolated from nonstimulated HuT-78 cells or cells stimulated for 30 min with anti-CD3/CD28 and GR was isolated from FP (10−8 M, 30 min) stimulated HuT-78 cells. These proteins were subsequently refolded in glycine solution. 100 µl of importin-α solution (100 ng/ml in TBS) was added to 96-well plates coated with goat anti-importin-α antibody. After 1 h incubation, GATA-3 (100 ng/ml in TBS) from stimulated or unstimulated cells were added to the importin-α-coated wells. GR (10 or 100 ng/ml in TBS) from stimulated or unstimulated cells were added to some wells. After a further 1 h incubation, the plate was washed and incubated with primary antibodies (a mixture of rabbit anti-GATA-3 and mouse anti-GR, Santa Cruz Biotechnology) for 1.5 h, and then incubated with secondary antibodies. FITC swine anti-rabbit IgG (Dako, Cambridge, UK) was used for the detection of GATA-3, and rhodamine donkey anti-mouse IgG (Novus, Littleton, Colorado, USA) was used for the detection of GR. FITC and rhodamine levels were measured with a fluorescent micro-plate reader (Bioline, London, UK)."}

{"project":"events-check-again","denotations":[{"id":"T6048","span":{"begin":0,"end":2},"obj":"Protein"},{"id":"T6049","span":{"begin":3,"end":9},"obj":"Protein"},{"id":"T6050","span":{"begin":259,"end":265},"obj":"Protein"},{"id":"T6051","span":{"begin":365,"end":367},"obj":"Protein"},{"id":"T6052","span":{"begin":636,"end":642},"obj":"Protein"},{"id":"T6053","span":{"begin":743,"end":745},"obj":"Protein"},{"id":"T6054","span":{"begin":1140,"end":1146},"obj":"Protein"},{"id":"T6055","span":{"begin":1248,"end":1250},"obj":"Protein"}],"text":"GR-GATA-3 In Vitro Competition Assay for Importin-α (Far-Western ELISA)\nImmunoprecipitated importin-α (anti-importin-α, Santa Cruz Biotechnology) from HuT-78 cells was separated by SDS-PAGE and purified from the excised gel by electroelution [36]. Similarly, GATA-3 was isolated from nonstimulated HuT-78 cells or cells stimulated for 30 min with anti-CD3/CD28 and GR was isolated from FP (10−8 M, 30 min) stimulated HuT-78 cells. These proteins were subsequently refolded in glycine solution. 100 µl of importin-α solution (100 ng/ml in TBS) was added to 96-well plates coated with goat anti-importin-α antibody. After 1 h incubation, GATA-3 (100 ng/ml in TBS) from stimulated or unstimulated cells were added to the importin-α-coated wells. GR (10 or 100 ng/ml in TBS) from stimulated or unstimulated cells were added to some wells. After a further 1 h incubation, the plate was washed and incubated with primary antibodies (a mixture of rabbit anti-GATA-3 and mouse anti-GR, Santa Cruz Biotechnology) for 1.5 h, and then incubated with secondary antibodies. FITC swine anti-rabbit IgG (Dako, Cambridge, UK) was used for the detection of GATA-3, and rhodamine donkey anti-mouse IgG (Novus, Littleton, Colorado, USA) was used for the detection of GR. FITC and rhodamine levels were measured with a fluorescent micro-plate reader (Bioline, London, UK)."}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T6056","span":{"begin":41,"end":51},"obj":"Protein"},{"id":"T6057","span":{"begin":91,"end":101},"obj":"Protein"},{"id":"T6058","span":{"begin":259,"end":265},"obj":"Protein"},{"id":"T6059","span":{"begin":352,"end":355},"obj":"Protein"},{"id":"T6060","span":{"begin":356,"end":360},"obj":"Protein"},{"id":"T6061","span":{"begin":365,"end":367},"obj":"Protein"},{"id":"T6062","span":{"begin":583,"end":612},"obj":"Protein"},{"id":"T6063","span":{"begin":718,"end":728},"obj":"Protein"},{"id":"T6064","span":{"begin":1061,"end":1087},"obj":"Protein"},{"id":"T6065","span":{"begin":1140,"end":1146},"obj":"Protein"},{"id":"T6066","span":{"begin":1180,"end":1183},"obj":"Protein"},{"id":"T6067","span":{"begin":1248,"end":1250},"obj":"Protein"},{"id":"T6068","span":{"begin":1127,"end":1136},"obj":"Gene_expression"},{"id":"T6069","span":{"begin":1235,"end":1244},"obj":"Gene_expression"},{"id":"T6070","span":{"begin":1114,"end":1118},"obj":"Positive_regulation"}],"relations":[{"id":"R5223","pred":"causeOf","subj":"T6064","obj":"T6070"},{"id":"R5224","pred":"themeOf","subj":"T6065","obj":"T6068"},{"id":"R5225","pred":"themeOf","subj":"T6067","obj":"T6069"},{"id":"R5226","pred":"themeOf","subj":"T6068","obj":"T6070"}],"text":"GR-GATA-3 In Vitro Competition Assay for Importin-α (Far-Western ELISA)\nImmunoprecipitated importin-α (anti-importin-α, Santa Cruz Biotechnology) from HuT-78 cells was separated by SDS-PAGE and purified from the excised gel by electroelution [36]. Similarly, GATA-3 was isolated from nonstimulated HuT-78 cells or cells stimulated for 30 min with anti-CD3/CD28 and GR was isolated from FP (10−8 M, 30 min) stimulated HuT-78 cells. These proteins were subsequently refolded in glycine solution. 100 µl of importin-α solution (100 ng/ml in TBS) was added to 96-well plates coated with goat anti-importin-α antibody. After 1 h incubation, GATA-3 (100 ng/ml in TBS) from stimulated or unstimulated cells were added to the importin-α-coated wells. GR (10 or 100 ng/ml in TBS) from stimulated or unstimulated cells were added to some wells. After a further 1 h incubation, the plate was washed and incubated with primary antibodies (a mixture of rabbit anti-GATA-3 and mouse anti-GR, Santa Cruz Biotechnology) for 1.5 h, and then incubated with secondary antibodies. FITC swine anti-rabbit IgG (Dako, Cambridge, UK) was used for the detection of GATA-3, and rhodamine donkey anti-mouse IgG (Novus, Littleton, Colorado, USA) was used for the detection of GR. FITC and rhodamine levels were measured with a fluorescent micro-plate reader (Bioline, London, UK)."}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T5481","span":{"begin":0,"end":2},"obj":"Protein"},{"id":"T5482","span":{"begin":3,"end":9},"obj":"Protein"},{"id":"T5483","span":{"begin":259,"end":265},"obj":"Protein"},{"id":"T5484","span":{"begin":365,"end":367},"obj":"Protein"},{"id":"T5485","span":{"begin":636,"end":642},"obj":"Protein"},{"id":"T5486","span":{"begin":743,"end":745},"obj":"Protein"},{"id":"T5487","span":{"begin":1140,"end":1146},"obj":"Protein"},{"id":"T5488","span":{"begin":1248,"end":1250},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"GR-GATA-3 In Vitro Competition Assay for Importin-α (Far-Western ELISA)\nImmunoprecipitated importin-α (anti-importin-α, Santa Cruz Biotechnology) from HuT-78 cells was separated by SDS-PAGE and purified from the excised gel by electroelution [36]. Similarly, GATA-3 was isolated from nonstimulated HuT-78 cells or cells stimulated for 30 min with anti-CD3/CD28 and GR was isolated from FP (10−8 M, 30 min) stimulated HuT-78 cells. These proteins were subsequently refolded in glycine solution. 100 µl of importin-α solution (100 ng/ml in TBS) was added to 96-well plates coated with goat anti-importin-α antibody. After 1 h incubation, GATA-3 (100 ng/ml in TBS) from stimulated or unstimulated cells were added to the importin-α-coated wells. GR (10 or 100 ng/ml in TBS) from stimulated or unstimulated cells were added to some wells. After a further 1 h incubation, the plate was washed and incubated with primary antibodies (a mixture of rabbit anti-GATA-3 and mouse anti-GR, Santa Cruz Biotechnology) for 1.5 h, and then incubated with secondary antibodies. FITC swine anti-rabbit IgG (Dako, Cambridge, UK) was used for the detection of GATA-3, and rhodamine donkey anti-mouse IgG (Novus, Littleton, Colorado, USA) was used for the detection of GR. FITC and rhodamine levels were measured with a fluorescent micro-plate reader (Bioline, London, UK)."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T5739","span":{"begin":0,"end":2},"obj":"P04150"},{"id":"T5740","span":{"begin":3,"end":9},"obj":"P23771"},{"id":"T5741","span":{"begin":259,"end":265},"obj":"P23771"},{"id":"T5742","span":{"begin":352,"end":355},"obj":"P04234"},{"id":"T5743","span":{"begin":352,"end":355},"obj":"P09693"},{"id":"T5744","span":{"begin":352,"end":355},"obj":"P20963"},{"id":"T5745","span":{"begin":352,"end":355},"obj":"P07766"},{"id":"T5746","span":{"begin":356,"end":360},"obj":"P10747"},{"id":"T5747","span":{"begin":365,"end":367},"obj":"P04150"},{"id":"T5748","span":{"begin":636,"end":642},"obj":"P23771"},{"id":"T5749","span":{"begin":743,"end":745},"obj":"P04150"},{"id":"T5750","span":{"begin":952,"end":958},"obj":"P23771"},{"id":"T5751","span":{"begin":974,"end":976},"obj":"P04150"},{"id":"T5752","span":{"begin":1140,"end":1146},"obj":"P23771"},{"id":"T5753","span":{"begin":1248,"end":1250},"obj":"P04150"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"GR-GATA-3 In Vitro Competition Assay for Importin-α (Far-Western ELISA)\nImmunoprecipitated importin-α (anti-importin-α, Santa Cruz Biotechnology) from HuT-78 cells was separated by SDS-PAGE and purified from the excised gel by electroelution [36]. Similarly, GATA-3 was isolated from nonstimulated HuT-78 cells or cells stimulated for 30 min with anti-CD3/CD28 and GR was isolated from FP (10−8 M, 30 min) stimulated HuT-78 cells. These proteins were subsequently refolded in glycine solution. 100 µl of importin-α solution (100 ng/ml in TBS) was added to 96-well plates coated with goat anti-importin-α antibody. After 1 h incubation, GATA-3 (100 ng/ml in TBS) from stimulated or unstimulated cells were added to the importin-α-coated wells. GR (10 or 100 ng/ml in TBS) from stimulated or unstimulated cells were added to some wells. After a further 1 h incubation, the plate was washed and incubated with primary antibodies (a mixture of rabbit anti-GATA-3 and mouse anti-GR, Santa Cruz Biotechnology) for 1.5 h, and then incubated with secondary antibodies. FITC swine anti-rabbit IgG (Dako, Cambridge, UK) was used for the detection of GATA-3, and rhodamine donkey anti-mouse IgG (Novus, Littleton, Colorado, USA) was used for the detection of GR. FITC and rhodamine levels were measured with a fluorescent micro-plate reader (Bioline, London, UK)."}

{"project":"test2","denotations":[{"id":"T5473","span":{"begin":0,"end":2},"obj":"Protein"},{"id":"T5474","span":{"begin":3,"end":9},"obj":"Protein"},{"id":"T5475","span":{"begin":259,"end":265},"obj":"Protein"},{"id":"T5476","span":{"begin":365,"end":367},"obj":"Protein"},{"id":"T5477","span":{"begin":636,"end":642},"obj":"Protein"},{"id":"T5478","span":{"begin":743,"end":745},"obj":"Protein"},{"id":"T5479","span":{"begin":1140,"end":1146},"obj":"Protein"},{"id":"T5480","span":{"begin":1248,"end":1250},"obj":"Protein"}],"text":"GR-GATA-3 In Vitro Competition Assay for Importin-α (Far-Western ELISA)\nImmunoprecipitated importin-α (anti-importin-α, Santa Cruz Biotechnology) from HuT-78 cells was separated by SDS-PAGE and purified from the excised gel by electroelution [36]. Similarly, GATA-3 was isolated from nonstimulated HuT-78 cells or cells stimulated for 30 min with anti-CD3/CD28 and GR was isolated from FP (10−8 M, 30 min) stimulated HuT-78 cells. These proteins were subsequently refolded in glycine solution. 100 µl of importin-α solution (100 ng/ml in TBS) was added to 96-well plates coated with goat anti-importin-α antibody. After 1 h incubation, GATA-3 (100 ng/ml in TBS) from stimulated or unstimulated cells were added to the importin-α-coated wells. GR (10 or 100 ng/ml in TBS) from stimulated or unstimulated cells were added to some wells. After a further 1 h incubation, the plate was washed and incubated with primary antibodies (a mixture of rabbit anti-GATA-3 and mouse anti-GR, Santa Cruz Biotechnology) for 1.5 h, and then incubated with secondary antibodies. FITC swine anti-rabbit IgG (Dako, Cambridge, UK) was used for the detection of GATA-3, and rhodamine donkey anti-mouse IgG (Novus, Littleton, Colorado, USA) was used for the detection of GR. FITC and rhodamine levels were measured with a fluorescent micro-plate reader (Bioline, London, UK)."}