PMC:2674207 / 15127-16052
Annnotations
2_test
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
19436703-11395507-86274489 | 140-142 | 11395507 | denotes | 35 |
T8362 | 140-142 | 11395507 | denotes | 35 |
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T4853 | 0-4 | NNP | denotes | Cell |
T4854 | 5-18 | NNP | denotes | Fractionation |
T4855 | 18-19 | -COMMA- | denotes | , |
T4856 | 20-39 | NN | denotes | Immunoprecipitation |
T4857 | 39-40 | -COMMA- | denotes | , |
T4858 | 41-44 | CC | denotes | and |
T4859 | 45-52 | NNP | denotes | Western |
T4860 | 53-57 | NNP | denotes | Blot |
T4861 | 58-66 | NN | denotes | Analysis |
T4862 | 67-74 | JJ | denotes | Nuclear |
T4863 | 75-78 | CC | denotes | and |
T4864 | 79-90 | JJ | denotes | cytoplasmic |
T4865 | 91-100 | NN | denotes | fractions |
T4866 | 101-105 | VB | denotes | were |
T4867 | 106-114 | VB | denotes | prepared |
T4868 | 115-117 | IN | denotes | as |
T4869 | 118-128 | RB | denotes | previously |
T4870 | 129-138 | VB | denotes | described |
T4871 | 139-140 | -LRB- | denotes | [ |
T4872 | 140-142 | CD | denotes | 35 |
T4873 | 142-143 | -RRB- | denotes | ] |
T4874 | 145-150 | JJ | denotes | Whole |
T4875 | 151-155 | NN | denotes | cell |
T4876 | 156-163 | NN | denotes | lysates |
T4877 | 164-168 | VB | denotes | were |
T4878 | 169-177 | VB | denotes | prepared |
T4879 | 178-180 | IN | denotes | in |
T4880 | 181-186 | NN | denotes | NP-40 |
T4881 | 187-192 | NN | denotes | lysis |
T4882 | 193-199 | NN | denotes | buffer |
T4883 | 200-201 | -LRB- | denotes | ( |
T4884 | 201-204 | CD | denotes | 0.5 |
T4885 | 204-205 | NN | denotes | % |
T4886 | 206-213 | NN | denotes | Nonidet |
T4887 | 214-218 | NN | denotes | P-40 |
T4888 | 218-219 | -COMMA- | denotes | , |
T4889 | 220-222 | CD | denotes | 20 |
T4890 | 223-225 | NN | denotes | mM |
T4891 | 226-234 | NN | denotes | Tris-HCl |
T4892 | 235-236 | -LRB- | denotes | [ |
T4893 | 236-238 | NN | denotes | pH |
T4894 | 239-242 | CD | denotes | 7.5 |
T4895 | 242-243 | -RRB- | denotes | ] |
T4896 | 243-244 | -COMMA- | denotes | , |
T4897 | 245-248 | CD | denotes | 150 |
T4898 | 249-251 | NN | denotes | mM |
T4899 | 252-256 | NN | denotes | NaCl |
T4900 | 256-257 | -RRB- | denotes | ) |
T4901 | 258-260 | IN | denotes | in |
T4902 | 261-264 | DT | denotes | the |
T4903 | 265-273 | NN | denotes | presence |
T4904 | 274-276 | IN | denotes | of |
T4905 | 277-285 | JJ | denotes | complete |
T4906 | 286-294 | NN | denotes | protease |
T4907 | 295-303 | NN | denotes | cocktail |
T4908 | 304-313 | NN | denotes | inhibitor |
T4909 | 315-322 | NN | denotes | Lysates |
T4910 | 323-327 | VB | denotes | were |
T4911 | 328-339 | VB | denotes | centrifuged |
T4912 | 340-342 | IN | denotes | at |
T4913 | 343-346 | NN | denotes | 4°C |
T4914 | 347-350 | IN | denotes | for |
T4915 | 351-353 | CD | denotes | 10 |
T4916 | 354-357 | NN | denotes | min |
T4917 | 358-360 | IN | denotes | at |
T4918 | 361-367 | CD | denotes | 12,000 |
T4919 | 368-371 | NN | denotes | rpm |
T4920 | 372-374 | IN | denotes | in |
T4921 | 375-377 | DT | denotes | an |
T4922 | 378-387 | NNP | denotes | Eppendorf |
T4923 | 388-403 | NN | denotes | microcentrifuge |
T4924 | 404-406 | TO | denotes | to |
T4925 | 407-413 | VB | denotes | remove |
T4926 | 414-422 | JJ | denotes | cellular |
T4927 | 423-429 | NN | denotes | debris |
T4928 | 431-438 | NN | denotes | Samples |
T4929 | 439-443 | VB | denotes | were |
T4930 | 444-448 | RB | denotes | then |
T4931 | 449-467 | VB | denotes | immunoprecipitated |
T4932 | 468-472 | IN | denotes | with |
T4933 | 473-479 | CC | denotes | either |
T4934 | 480-482 | CD | denotes | 10 |
T4935 | 483-485 | NN | denotes | µl |
T4936 | 486-488 | IN | denotes | of |
T4937 | 489-497 | NN | denotes | antibody |
T4938 | 498-505 | IN | denotes | against |
T4939 | 506-512 | NN | denotes | GATA-3 |
T4940 | 513-515 | CC | denotes | or |
T4941 | 516-526 | NN | denotes | importin-α |
T4942 | 527-532 | VB | denotes | using |
T4943 | 533-536 | NN | denotes | A/G |
T4944 | 537-544 | VB | denotes | agarose |
T4945 | 545-551 | NN | denotes | slurry |
T4946 | 552-554 | IN | denotes | in |
T4947 | 555-558 | DT | denotes | the |
T4948 | 559-567 | NN | denotes | presence |
T4949 | 568-570 | IN | denotes | of |
T4950 | 571-579 | NN | denotes | protease |
T4951 | 580-589 | NN | denotes | inhibitor |
T4952 | 590-595 | VB | denotes | using |
T4953 | 596-599 | DT | denotes | the |
T4954 | 600-605 | NNP | denotes | Catch |
T4955 | 606-609 | CC | denotes | and |
T4956 | 610-617 | NN | denotes | Release |
T4957 | 618-629 | NN | denotes | methodology |
T4958 | 630-631 | -LRB- | denotes | ( |
T4959 | 631-638 | NNP | denotes | Upstate |
T4960 | 639-652 | NNP | denotes | Biotechnology |
T4961 | 652-653 | -COMMA- | denotes | , |
T4962 | 654-658 | NNP | denotes | Lake |
T4963 | 659-665 | NNP | denotes | Placid |
T4964 | 665-666 | -COMMA- | denotes | , |
T4965 | 667-670 | NNP | denotes | New |
T4966 | 671-675 | NNP | denotes | York |
T4967 | 675-676 | -COMMA- | denotes | , |
T4968 | 677-680 | NNP | denotes | USA |
T4969 | 680-681 | -RRB- | denotes | ) |
T4970 | 683-690 | NN | denotes | Western |
T4971 | 691-695 | NN | denotes | blot |
T4972 | 696-704 | NN | denotes | analysis |
T4973 | 705-708 | VB | denotes | was |
T4974 | 709-718 | VB | denotes | performed |
T4975 | 719-724 | VB | denotes | using |
T4976 | 725-736 | NN | denotes | anti-GATA-3 |
T4977 | 736-737 | -COMMA- | denotes | , |
T4978 | 738-753 | JJ | denotes | anti-importin-α |
T4979 | 753-754 | -COMMA- | denotes | , |
T4980 | 755-762 | JJ | denotes | anti-GR |
T4981 | 762-763 | -COMMA- | denotes | , |
T4982 | 764-774 | JJ | denotes | anti-p-p38 |
T4983 | 775-778 | NN | denotes | MAP |
T4984 | 779-785 | NN | denotes | kinase |
T4985 | 785-786 | -COMMA- | denotes | , |
T4986 | 787-799 | NN | denotes | anti-p-ATF-2 |
T4987 | 799-800 | -COMMA- | denotes | , |
T4988 | 801-804 | CC | denotes | and |
T4989 | 805-818 | JJ | denotes | anti-p-serine |
T4990 | 820-834 | JJ | denotes | Immunoreactive |
T4991 | 835-843 | NN | denotes | proteins |
T4992 | 844-848 | VB | denotes | were |
T4993 | 849-857 | VB | denotes | detected |
T4994 | 858-863 | VB | denotes | using |
T4995 | 864-866 | DT | denotes | an |
T4996 | 867-875 | VB | denotes | enhanced |
T4997 | 876-893 | NN | denotes | chemiluminescence |
T4998 | 894-897 | NN | denotes | ECL |
T4999 | 898-901 | NN | denotes | kit |
T5000 | 902-903 | -LRB- | denotes | ( |
T5001 | 903-911 | NNP | denotes | Amersham |
T5002 | 912-923 | NNP | denotes | Biosciences |
T5003 | 923-924 | -RRB- | denotes | ) |
R4166 | T4854 | T4853 | arg1Of | Fractionation,Cell |
R4167 | T4854 | T4855 | arg1Of | Fractionation,"," |
R4168 | T4855 | T4858 | arg1Of | ",",and |
R4169 | T4856 | T4855 | arg2Of | Immunoprecipitation,"," |
R4170 | T4858 | T4857 | arg1Of | and,"," |
R4171 | T4860 | T4859 | arg1Of | Blot,Western |
R4172 | T4861 | T4858 | arg2Of | Analysis,and |
R4173 | T4861 | T4860 | arg1Of | Analysis,Blot |
R4174 | T4862 | T4863 | arg1Of | Nuclear,and |
R4175 | T4864 | T4863 | arg2Of | cytoplasmic,and |
R4176 | T4865 | T4862 | arg1Of | fractions,Nuclear |
R4177 | T4865 | T4864 | arg1Of | fractions,cytoplasmic |
R4178 | T4865 | T4866 | arg1Of | fractions,were |
R4179 | T4865 | T4867 | arg2Of | fractions,prepared |
R4180 | T4867 | T4866 | arg2Of | prepared,were |
R4181 | T4867 | T4868 | arg1Of | prepared,as |
R4182 | T4870 | T4869 | arg1Of | described,previously |
R4183 | T4872 | T4868 | arg2Of | 35,as |
R4184 | T4872 | T4870 | arg1Of | 35,described |
R4185 | T4872 | T4871 | arg2Of | 35,[ |
R4186 | T4873 | T4871 | arg3Of | ],[ |
R4187 | T4876 | T4874 | arg1Of | lysates,Whole |
R4188 | T4876 | T4875 | arg1Of | lysates,cell |
R4189 | T4876 | T4877 | arg1Of | lysates,were |
R4190 | T4876 | T4878 | arg2Of | lysates,prepared |
R4191 | T4878 | T4877 | arg2Of | prepared,were |
R4192 | T4878 | T4879 | arg1Of | prepared,in |
R4193 | T4882 | T4879 | arg2Of | buffer,in |
R4194 | T4882 | T4880 | arg1Of | buffer,NP-40 |
R4195 | T4882 | T4881 | arg1Of | buffer,lysis |
R4196 | T4882 | T4883 | arg1Of | buffer,( |
R4197 | T4882 | T4901 | arg1Of | buffer,in |
R4198 | T4884 | T4885 | arg1Of | 0.5,% |
R4199 | T4887 | T4884 | arg1Of | P-40,0.5 |
R4200 | T4887 | T4886 | arg1Of | P-40,Nonidet |
R4201 | T4887 | T4888 | arg1Of | P-40,"," |
R4202 | T4888 | T4896 | arg1Of | ",","," |
R4203 | T4889 | T4890 | arg1Of | 20,mM |
R4204 | T4891 | T4888 | arg2Of | Tris-HCl,"," |
R4205 | T4891 | T4889 | arg1Of | Tris-HCl,20 |
R4206 | T4891 | T4892 | arg1Of | Tris-HCl,[ |
R4207 | T4893 | T4892 | arg2Of | pH,[ |
R4208 | T4893 | T4894 | arg1Of | pH,7.5 |
R4209 | T4895 | T4892 | arg3Of | ],[ |
R4210 | T4896 | T4883 | arg2Of | ",",( |
R4211 | T4897 | T4898 | arg1Of | 150,mM |
R4212 | T4899 | T4896 | arg2Of | NaCl,"," |
R4213 | T4899 | T4897 | arg1Of | NaCl,150 |
R4214 | T4900 | T4883 | arg3Of | ),( |
R4215 | T4903 | T4901 | arg2Of | presence,in |
R4216 | T4903 | T4902 | arg1Of | presence,the |
R4217 | T4903 | T4904 | arg1Of | presence,of |
R4218 | T4908 | T4904 | arg2Of | inhibitor,of |
R4219 | T4908 | T4905 | arg1Of | inhibitor,complete |
R4220 | T4908 | T4906 | arg1Of | inhibitor,protease |
R4221 | T4908 | T4907 | arg1Of | inhibitor,cocktail |
R4222 | T4909 | T4910 | arg1Of | Lysates,were |
R4223 | T4909 | T4911 | arg2Of | Lysates,centrifuged |
R4224 | T4909 | T4925 | arg1Of | Lysates,remove |
R4225 | T4911 | T4910 | arg2Of | centrifuged,were |
R4226 | T4911 | T4912 | arg1Of | centrifuged,at |
R4227 | T4911 | T4914 | arg1Of | centrifuged,for |
R4228 | T4911 | T4917 | arg1Of | centrifuged,at |
R4229 | T4911 | T4920 | arg1Of | centrifuged,in |
R4230 | T4911 | T4924 | modOf | centrifuged,to |
R4231 | T4913 | T4912 | arg2Of | 4°C,at |
R4232 | T4916 | T4914 | arg2Of | min,for |
R4233 | T4916 | T4915 | arg1Of | min,10 |
R4234 | T4919 | T4917 | arg2Of | rpm,at |
R4235 | T4919 | T4918 | arg1Of | rpm,"12,000" |
R4236 | T4923 | T4920 | arg2Of | microcentrifuge,in |
R4237 | T4923 | T4921 | arg1Of | microcentrifuge,an |
R4238 | T4923 | T4922 | arg1Of | microcentrifuge,Eppendorf |
R4239 | T4925 | T4924 | arg1Of | remove,to |
R4240 | T4927 | T4925 | arg2Of | debris,remove |
R4241 | T4927 | T4926 | arg1Of | debris,cellular |
R4242 | T4928 | T4929 | arg1Of | Samples,were |
R4243 | T4928 | T4931 | arg2Of | Samples,immunoprecipitated |
R4244 | T4931 | T4929 | arg2Of | immunoprecipitated,were |
R4245 | T4931 | T4930 | arg1Of | immunoprecipitated,then |
R4246 | T4931 | T4932 | arg1Of | immunoprecipitated,with |
R4247 | T4935 | T4932 | arg2Of | µl,with |
R4248 | T4935 | T4933 | arg1Of | µl,either |
R4249 | T4935 | T4934 | arg1Of | µl,10 |
R4250 | T4935 | T4936 | arg1Of | µl,of |
R4251 | T4935 | T4942 | arg1Of | µl,using |
R4252 | T4935 | T4952 | arg1Of | µl,using |
R4253 | T4935 | T4958 | arg1Of | µl,( |
R4254 | T4937 | T4936 | arg2Of | antibody,of |
R4255 | T4937 | T4938 | arg1Of | antibody,against |
R4256 | T4939 | T4940 | arg1Of | GATA-3,or |
R4257 | T4940 | T4938 | arg2Of | or,against |
R4258 | T4941 | T4940 | arg2Of | importin-α,or |
R4259 | T4942 | T4946 | arg1Of | using,in |
R4260 | T4942 | T4952 | modOf | using,using |
R4261 | T4945 | T4942 | arg2Of | slurry,using |
R4262 | T4945 | T4943 | arg1Of | slurry,A/G |
R4263 | T4945 | T4944 | arg2Of | slurry,agarose |
R4264 | T4948 | T4946 | arg2Of | presence,in |
R4265 | T4948 | T4947 | arg1Of | presence,the |
R4266 | T4948 | T4949 | arg1Of | presence,of |
R4267 | T4951 | T4949 | arg2Of | inhibitor,of |
R4268 | T4951 | T4950 | arg1Of | inhibitor,protease |
R4269 | T4954 | T4955 | arg1Of | Catch,and |
R4270 | T4956 | T4955 | arg2Of | Release,and |
R4271 | T4957 | T4952 | arg2Of | methodology,using |
R4272 | T4957 | T4953 | arg1Of | methodology,the |
R4273 | T4957 | T4954 | arg1Of | methodology,Catch |
R4274 | T4957 | T4956 | arg1Of | methodology,Release |
R4275 | T4960 | T4959 | arg1Of | Biotechnology,Upstate |
R4276 | T4960 | T4961 | arg1Of | Biotechnology,"," |
R4277 | T4961 | T4964 | arg1Of | ",","," |
R4278 | T4963 | T4961 | arg2Of | Placid,"," |
R4279 | T4963 | T4962 | arg1Of | Placid,Lake |
R4280 | T4964 | T4958 | arg2Of | ",",( |
R4281 | T4964 | T4967 | arg1Of | ",","," |
R4282 | T4964 | T4968 | arg1Of | ",",USA |
R4283 | T4966 | T4964 | arg2Of | York,"," |
R4284 | T4966 | T4965 | arg1Of | York,New |
R4285 | T4969 | T4958 | arg3Of | ),( |
R4286 | T4972 | T4970 | arg1Of | analysis,Western |
R4287 | T4972 | T4971 | arg1Of | analysis,blot |
R4288 | T4972 | T4973 | arg1Of | analysis,was |
R4289 | T4972 | T4974 | arg2Of | analysis,performed |
R4290 | T4974 | T4973 | arg2Of | performed,was |
R4291 | T4975 | T4974 | arg3Of | using,performed |
R4292 | T4976 | T4975 | arg2Of | anti-GATA-3,using |
R4293 | T4976 | T4977 | arg1Of | anti-GATA-3,"," |
R4294 | T4978 | T4979 | arg1Of | anti-importin-α,"," |
R4295 | T4979 | T4981 | arg1Of | ",","," |
R4296 | T4980 | T4979 | arg2Of | anti-GR,"," |
R4297 | T4982 | T4981 | arg2Of | anti-p-p38,"," |
R4298 | T4984 | T4978 | arg1Of | kinase,anti-importin-α |
R4299 | T4984 | T4980 | arg1Of | kinase,anti-GR |
R4300 | T4984 | T4982 | arg1Of | kinase,anti-p-p38 |
R4301 | T4984 | T4983 | arg1Of | kinase,MAP |
R4302 | T4984 | T4985 | arg1Of | kinase,"," |
R4303 | T4985 | T4988 | arg1Of | ",",and |
R4304 | T4986 | T4985 | arg2Of | anti-p-ATF-2,"," |
R4305 | T4988 | T4977 | arg2Of | and,"," |
R4306 | T4988 | T4987 | arg1Of | and,"," |
R4307 | T4989 | T4988 | arg2Of | anti-p-serine,and |
R4308 | T4991 | T4990 | arg1Of | proteins,Immunoreactive |
R4309 | T4991 | T4992 | arg1Of | proteins,were |
R4310 | T4991 | T4993 | arg2Of | proteins,detected |
R4311 | T4993 | T4992 | arg2Of | detected,were |
R4312 | T4994 | T4993 | arg3Of | using,detected |
R4313 | T4999 | T4994 | arg2Of | kit,using |
R4314 | T4999 | T4995 | arg1Of | kit,an |
R4315 | T4999 | T4996 | arg2Of | kit,enhanced |
R4316 | T4999 | T4997 | arg1Of | kit,chemiluminescence |
R4317 | T4999 | T4998 | arg1Of | kit,ECL |
R4318 | T4999 | T5000 | arg1Of | kit,( |
R4319 | T5002 | T5000 | arg2Of | Biosciences,( |
R4320 | T5002 | T5001 | arg1Of | Biosciences,Amersham |
R4321 | T5003 | T5000 | arg3Of | ),( |
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5013 | 0-4 | NNP | denotes | Cell |
T5014 | 5-18 | NNP | denotes | Fractionation |
T5015 | 18-19 | , | denotes | , |
T5016 | 20-39 | NNP | denotes | Immunoprecipitation |
T5017 | 39-40 | , | denotes | , |
T5018 | 41-44 | CC | denotes | and |
T5019 | 45-52 | JJ | denotes | Western |
T5020 | 53-57 | NNP | denotes | Blot |
T5021 | 58-66 | NNP | denotes | Analysis |
T5022 | 67-74 | NNP | denotes | Nuclear |
T5023 | 75-78 | CC | denotes | and |
T5024 | 79-90 | JJ | denotes | cytoplasmic |
T5025 | 91-100 | NNS | denotes | fractions |
T5026 | 101-105 | VBD | denotes | were |
T5027 | 106-114 | VBN | denotes | prepared |
T5028 | 115-117 | IN | denotes | as |
T5029 | 118-128 | RB | denotes | previously |
T5030 | 129-138 | VBN | denotes | described |
T5031 | 139-140 | NNP | denotes | [ |
T5032 | 140-142 | CD | denotes | 35 |
T5033 | 142-143 | NNP | denotes | ] |
T5034 | 143-144 | . | denotes | . |
T5035 | 145-150 | JJ | denotes | Whole |
T5036 | 151-155 | NN | denotes | cell |
T5037 | 156-163 | NNS | denotes | lysates |
T5038 | 164-168 | VBD | denotes | were |
T5039 | 169-177 | VBN | denotes | prepared |
T5040 | 178-180 | IN | denotes | in |
T5041 | 181-186 | NN | denotes | NP-40 |
T5042 | 187-192 | NN | denotes | lysis |
T5043 | 193-199 | NN | denotes | buffer |
T5044 | 200-201 | -LRB- | denotes | ( |
T5045 | 201-204 | CD | denotes | 0.5 |
T5046 | 204-205 | NN | denotes | % |
T5047 | 206-213 | NNP | denotes | Nonidet |
T5048 | 214-218 | NNP | denotes | P-40 |
T5049 | 218-219 | , | denotes | , |
T5050 | 220-222 | CD | denotes | 20 |
T5051 | 223-225 | NNP | denotes | mM |
T5052 | 226-234 | NNP | denotes | Tris-HCl |
T5053 | 235-236 | NNP | denotes | [ |
T5054 | 236-238 | NNP | denotes | pH |
T5055 | 239-242 | CD | denotes | 7.5 |
T5056 | 242-243 | NNP | denotes | ] |
T5057 | 243-244 | , | denotes | , |
T5058 | 245-248 | CD | denotes | 150 |
T5059 | 249-251 | NNP | denotes | mM |
T5060 | 252-256 | NNP | denotes | NaCl |
T5061 | 256-257 | -RRB- | denotes | ) |
T5062 | 258-260 | IN | denotes | in |
T5063 | 261-264 | DT | denotes | the |
T5064 | 265-273 | NN | denotes | presence |
T5065 | 274-276 | IN | denotes | of |
T5066 | 277-285 | JJ | denotes | complete |
T5067 | 286-294 | NN | denotes | protease |
T5068 | 295-303 | NN | denotes | cocktail |
T5069 | 304-313 | NN | denotes | inhibitor |
T5070 | 313-314 | . | denotes | . |
T5071 | 315-322 | NNS | denotes | Lysates |
T5072 | 323-327 | VBD | denotes | were |
T5073 | 328-339 | VBN | denotes | centrifuged |
T5074 | 340-342 | IN | denotes | at |
T5075 | 343-344 | CD | denotes | 4 |
T5076 | 344-345 | CD | denotes | ° |
T5077 | 345-346 | NNP | denotes | C |
T5078 | 347-350 | IN | denotes | for |
T5079 | 351-353 | CD | denotes | 10 |
T5080 | 354-357 | NN | denotes | min |
T5081 | 358-360 | IN | denotes | at |
T5082 | 361-367 | CD | denotes | 12,000 |
T5083 | 368-371 | NN | denotes | rpm |
T5084 | 372-374 | IN | denotes | in |
T5085 | 375-377 | DT | denotes | an |
T5086 | 378-387 | NNP | denotes | Eppendorf |
T5087 | 388-403 | NN | denotes | microcentrifuge |
T5088 | 404-406 | TO | denotes | to |
T5089 | 407-413 | VB | denotes | remove |
T5090 | 414-422 | JJ | denotes | cellular |
T5091 | 423-429 | NN | denotes | debris |
T5092 | 429-430 | . | denotes | . |
T5093 | 431-438 | NNS | denotes | Samples |
T5094 | 439-443 | VBD | denotes | were |
T5095 | 444-448 | RB | denotes | then |
T5096 | 449-467 | VBN | denotes | immunoprecipitated |
T5097 | 468-472 | IN | denotes | with |
T5098 | 473-479 | DT | denotes | either |
T5099 | 480-482 | CD | denotes | 10 |
T5100 | 483-485 | NN | denotes | µl |
T5101 | 486-488 | IN | denotes | of |
T5102 | 489-497 | NN | denotes | antibody |
T5103 | 498-505 | IN | denotes | against |
T5104 | 506-512 | NNP | denotes | GATA-3 |
T5105 | 513-515 | CC | denotes | or |
T5106 | 516-526 | JJ | denotes | importin-α |
T5107 | 527-532 | VBG | denotes | using |
T5108 | 533-536 | NNP | denotes | A/G |
T5109 | 537-544 | JJ | denotes | agarose |
T5110 | 545-551 | NN | denotes | slurry |
T5111 | 552-554 | IN | denotes | in |
T5112 | 555-558 | DT | denotes | the |
T5113 | 559-567 | NN | denotes | presence |
T5114 | 568-570 | IN | denotes | of |
T5115 | 571-579 | NN | denotes | protease |
T5116 | 580-589 | NN | denotes | inhibitor |
T5117 | 590-595 | VBG | denotes | using |
T5118 | 596-599 | DT | denotes | the |
T5119 | 600-605 | NNP | denotes | Catch |
T5120 | 606-609 | CC | denotes | and |
T5121 | 610-617 | NNP | denotes | Release |
T5122 | 618-629 | NN | denotes | methodology |
T5123 | 630-631 | -LRB- | denotes | ( |
T5124 | 631-638 | NNP | denotes | Upstate |
T5125 | 639-652 | NNP | denotes | Biotechnology |
T5126 | 652-653 | , | denotes | , |
T5127 | 654-658 | NNP | denotes | Lake |
T5128 | 659-665 | NNP | denotes | Placid |
T5129 | 665-666 | , | denotes | , |
T5130 | 667-670 | NNP | denotes | New |
T5131 | 671-675 | NNP | denotes | York |
T5132 | 675-676 | , | denotes | , |
T5133 | 677-680 | NNP | denotes | USA |
T5134 | 680-681 | -RRB- | denotes | ) |
T5135 | 681-682 | . | denotes | . |
T5136 | 683-690 | JJ | denotes | Western |
T5137 | 691-695 | NN | denotes | blot |
T5138 | 696-704 | NN | denotes | analysis |
T5139 | 705-708 | VBD | denotes | was |
T5140 | 709-718 | VBN | denotes | performed |
T5141 | 719-724 | VBG | denotes | using |
T5142 | 725-736 | JJ | denotes | anti-GATA-3 |
T5143 | 736-737 | , | denotes | , |
T5144 | 738-753 | JJ | denotes | anti-importin-α |
T5145 | 753-754 | , | denotes | , |
T5146 | 755-762 | JJ | denotes | anti-GR |
T5147 | 762-763 | , | denotes | , |
T5148 | 764-774 | JJ | denotes | anti-p-p38 |
T5149 | 775-778 | NNP | denotes | MAP |
T5150 | 779-785 | NN | denotes | kinase |
T5151 | 785-786 | , | denotes | , |
T5152 | 787-799 | JJ | denotes | anti-p-ATF-2 |
T5153 | 799-800 | , | denotes | , |
T5154 | 801-804 | CC | denotes | and |
T5155 | 805-818 | JJ | denotes | anti-p-serine |
T5156 | 818-819 | . | denotes | . |
T5157 | 820-834 | JJ | denotes | Immunoreactive |
T5158 | 835-843 | NNS | denotes | proteins |
T5159 | 844-848 | VBD | denotes | were |
T5160 | 849-857 | VBN | denotes | detected |
T5161 | 858-863 | VBG | denotes | using |
T5162 | 864-866 | DT | denotes | an |
T5163 | 867-875 | JJ | denotes | enhanced |
T5164 | 876-893 | NN | denotes | chemiluminescence |
T5165 | 894-897 | NNP | denotes | ECL |
T5166 | 898-901 | NN | denotes | kit |
T5167 | 902-903 | -LRB- | denotes | ( |
T5168 | 903-911 | NNP | denotes | Amersham |
T5169 | 912-923 | NNPS | denotes | Biosciences |
T5170 | 923-924 | -RRB- | denotes | ) |
T5171 | 924-925 | . | denotes | . |
R4322 | T5013 | T5014 | compound | Cell,Fractionation |
R4323 | T5014 | T5027 | nsubjpass | Fractionation,prepared |
R4324 | T5015 | T5014 | punct | ",",Fractionation |
R4325 | T5016 | T5014 | conj | Immunoprecipitation,Fractionation |
R4326 | T5017 | T5016 | punct | ",",Immunoprecipitation |
R4327 | T5018 | T5016 | cc | and,Immunoprecipitation |
R4328 | T5019 | T5022 | amod | Western,Nuclear |
R4329 | T5020 | T5022 | compound | Blot,Nuclear |
R4330 | T5021 | T5022 | compound | Analysis,Nuclear |
R4331 | T5022 | T5016 | conj | Nuclear,Immunoprecipitation |
R4332 | T5023 | T5022 | cc | and,Nuclear |
R4333 | T5024 | T5022 | conj | cytoplasmic,Nuclear |
R4334 | T5025 | T5014 | appos | fractions,Fractionation |
R4335 | T5026 | T5027 | auxpass | were,prepared |
R4336 | T5027 | T5027 | ROOT | prepared,prepared |
R4337 | T5028 | T5030 | mark | as,described |
R4338 | T5029 | T5030 | advmod | previously,described |
R4339 | T5030 | T5027 | advcl | described,prepared |
R4340 | T5031 | T5033 | nmod | [,] |
R4341 | T5032 | T5033 | nummod | 35,] |
R4342 | T5033 | T5030 | dobj | ],described |
R4343 | T5034 | T5027 | punct | .,prepared |
R4344 | T5035 | T5037 | amod | Whole,lysates |
R4345 | T5036 | T5037 | compound | cell,lysates |
R4346 | T5037 | T5039 | nsubjpass | lysates,prepared |
R4347 | T5038 | T5039 | auxpass | were,prepared |
R4348 | T5039 | T5039 | ROOT | prepared,prepared |
R4349 | T5040 | T5039 | prep | in,prepared |
R4350 | T5041 | T5043 | compound | NP-40,buffer |
R4351 | T5042 | T5043 | compound | lysis,buffer |
R4352 | T5043 | T5040 | pobj | buffer,in |
R4353 | T5044 | T5048 | punct | (,P-40 |
R4354 | T5045 | T5046 | nummod | 0.5,% |
R4355 | T5046 | T5048 | compound | %,P-40 |
R4356 | T5047 | T5048 | compound | Nonidet,P-40 |
R4357 | T5048 | T5043 | appos | P-40,buffer |
R4358 | T5049 | T5048 | punct | ",",P-40 |
R4359 | T5050 | T5054 | nummod | 20,pH |
R4360 | T5051 | T5054 | compound | mM,pH |
R4361 | T5052 | T5054 | compound | Tris-HCl,pH |
R4415 | T5106 | T5104 | conj | importin-α,GATA-3 |
R4438 | T5129 | T5128 | punct | ",",Placid |
R4439 | T5130 | T5131 | compound | New,York |
R4440 | T5131 | T5128 | conj | York,Placid |
R4441 | T5132 | T5131 | punct | ",",York |
R4442 | T5133 | T5131 | conj | USA,York |
R4443 | T5134 | T5125 | punct | ),Biotechnology |
R4444 | T5135 | T5096 | punct | .,immunoprecipitated |
R4445 | T5136 | T5138 | amod | Western,analysis |
R4446 | T5137 | T5138 | compound | blot,analysis |
R4447 | T5138 | T5140 | nsubjpass | analysis,performed |
R4448 | T5139 | T5140 | auxpass | was,performed |
R4449 | T5140 | T5140 | ROOT | performed,performed |
R4450 | T5141 | T5140 | advcl | using,performed |
R4451 | T5142 | T5141 | dobj | anti-GATA-3,using |
R4452 | T5143 | T5142 | punct | ",",anti-GATA-3 |
R4453 | T5144 | T5142 | conj | anti-importin-α,anti-GATA-3 |
R4454 | T5145 | T5144 | punct | ",",anti-importin-α |
R4455 | T5146 | T5144 | conj | anti-GR,anti-importin-α |
R4456 | T5147 | T5146 | punct | ",",anti-GR |
R4457 | T5148 | T5150 | amod | anti-p-p38,kinase |
R4458 | T5149 | T5150 | compound | MAP,kinase |
R4459 | T5150 | T5146 | conj | kinase,anti-GR |
R4460 | T5151 | T5150 | punct | ",",kinase |
R4461 | T5152 | T5150 | conj | anti-p-ATF-2,kinase |
R4462 | T5153 | T5152 | punct | ",",anti-p-ATF-2 |
R4463 | T5154 | T5152 | cc | and,anti-p-ATF-2 |
R4464 | T5155 | T5152 | conj | anti-p-serine,anti-p-ATF-2 |
R4465 | T5156 | T5140 | punct | .,performed |
R4466 | T5157 | T5158 | amod | Immunoreactive,proteins |
R4467 | T5158 | T5160 | nsubjpass | proteins,detected |
R4468 | T5159 | T5160 | auxpass | were,detected |
R4469 | T5160 | T5160 | ROOT | detected,detected |
R4470 | T5161 | T5160 | advcl | using,detected |
R4471 | T5162 | T5166 | det | an,kit |
R4472 | T5163 | T5166 | amod | enhanced,kit |
R4473 | T5164 | T5165 | compound | chemiluminescence,ECL |
R4474 | T5165 | T5166 | compound | ECL,kit |
R4475 | T5166 | T5161 | dobj | kit,using |
R4476 | T5167 | T5166 | punct | (,kit |
R4477 | T5168 | T5169 | compound | Amersham,Biosciences |
R4478 | T5169 | T5166 | appos | Biosciences,kit |
R4479 | T5170 | T5166 | punct | ),kit |
R4480 | T5171 | T5160 | punct | .,detected |
R4362 | T5053 | T5054 | compound | [,pH |
R4363 | T5054 | T5048 | appos | pH,P-40 |
R4364 | T5055 | T5056 | nummod | 7.5,] |
R4365 | T5056 | T5056 | ROOT | ],] |
R4366 | T5057 | T5056 | punct | ",",] |
R4367 | T5058 | T5060 | nummod | 150,NaCl |
R4368 | T5059 | T5060 | compound | mM,NaCl |
R4369 | T5060 | T5056 | appos | NaCl,] |
R4370 | T5061 | T5056 | punct | ),] |
R4371 | T5062 | T5056 | prep | in,] |
R4372 | T5063 | T5064 | det | the,presence |
R4373 | T5064 | T5062 | pobj | presence,in |
R4374 | T5065 | T5064 | prep | of,presence |
R4375 | T5066 | T5069 | amod | complete,inhibitor |
R4376 | T5067 | T5069 | compound | protease,inhibitor |
R4377 | T5068 | T5069 | compound | cocktail,inhibitor |
R4378 | T5069 | T5065 | pobj | inhibitor,of |
R4379 | T5070 | T5039 | punct | .,prepared |
R4380 | T5071 | T5073 | nsubjpass | Lysates,centrifuged |
R4381 | T5072 | T5073 | auxpass | were,centrifuged |
R4382 | T5073 | T5073 | ROOT | centrifuged,centrifuged |
R4383 | T5074 | T5073 | prep | at,centrifuged |
R4384 | T5075 | T5076 | nummod | 4,° |
R4385 | T5076 | T5074 | pobj | °,at |
R4386 | T5077 | T5073 | conj | C,centrifuged |
R4387 | T5078 | T5077 | prep | for,C |
R4388 | T5079 | T5080 | nummod | 10,min |
R4389 | T5080 | T5078 | pobj | min,for |
R4390 | T5081 | T5080 | prep | at,min |
R4391 | T5082 | T5083 | nummod | "12,000",rpm |
R4392 | T5083 | T5081 | pobj | rpm,at |
R4393 | T5084 | T5073 | prep | in,centrifuged |
R4394 | T5085 | T5087 | det | an,microcentrifuge |
R4395 | T5086 | T5087 | compound | Eppendorf,microcentrifuge |
R4396 | T5087 | T5084 | pobj | microcentrifuge,in |
R4397 | T5088 | T5089 | aux | to,remove |
R4398 | T5089 | T5073 | advcl | remove,centrifuged |
R4399 | T5090 | T5091 | amod | cellular,debris |
R4400 | T5091 | T5089 | dobj | debris,remove |
R4401 | T5092 | T5073 | punct | .,centrifuged |
R4402 | T5093 | T5096 | nsubjpass | Samples,immunoprecipitated |
R4403 | T5094 | T5096 | auxpass | were,immunoprecipitated |
R4404 | T5095 | T5096 | advmod | then,immunoprecipitated |
R4405 | T5096 | T5096 | ROOT | immunoprecipitated,immunoprecipitated |
R4406 | T5097 | T5096 | prep | with,immunoprecipitated |
R4407 | T5098 | T5100 | preconj | either,µl |
R4408 | T5099 | T5100 | nummod | 10,µl |
R4409 | T5100 | T5107 | nsubj | µl,using |
R4410 | T5101 | T5100 | prep | of,µl |
R4411 | T5102 | T5101 | pobj | antibody,of |
R4412 | T5103 | T5102 | prep | against,antibody |
R4413 | T5104 | T5103 | pobj | GATA-3,against |
R4414 | T5105 | T5104 | cc | or,GATA-3 |
R4416 | T5107 | T5097 | pcomp | using,with |
R4417 | T5108 | T5110 | nmod | A/G,slurry |
R4418 | T5109 | T5110 | amod | agarose,slurry |
R4419 | T5110 | T5107 | dobj | slurry,using |
R4420 | T5111 | T5107 | prep | in,using |
R4421 | T5112 | T5113 | det | the,presence |
R4422 | T5113 | T5111 | pobj | presence,in |
R4423 | T5114 | T5113 | prep | of,presence |
R4424 | T5115 | T5116 | amod | protease,inhibitor |
R4425 | T5116 | T5114 | pobj | inhibitor,of |
R4426 | T5117 | T5107 | advcl | using,using |
R4427 | T5118 | T5119 | det | the,Catch |
R4428 | T5119 | T5117 | dobj | Catch,using |
R4429 | T5120 | T5119 | cc | and,Catch |
R4430 | T5121 | T5119 | conj | Release,Catch |
R4431 | T5122 | T5119 | conj | methodology,Catch |
R4432 | T5123 | T5125 | punct | (,Biotechnology |
R4433 | T5124 | T5125 | compound | Upstate,Biotechnology |
R4434 | T5125 | T5119 | conj | Biotechnology,Catch |
R4435 | T5126 | T5125 | punct | ",",Biotechnology |
R4436 | T5127 | T5128 | compound | Lake,Placid |
R4437 | T5128 | T5125 | conj | Placid,Biotechnology |
GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5179 | 187-192 | http://purl.obolibrary.org/obo/GO_0019835 | denotes | lysis |
T5180 | 414-422 | http://purl.obolibrary.org/obo/GO_0007349 | denotes | cellular |
GO-MF
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5181 | 489-497 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibody |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5182 | 0-4 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | Cell |
T5183 | 151-155 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
T5184 | 489-497 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibody |
T5185 | 489-497 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibody |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T4846 | 0-66 | Sentence | denotes | Cell Fractionation, Immunoprecipitation, and Western Blot Analysis |
T4847 | 67-144 | Sentence | denotes | Nuclear and cytoplasmic fractions were prepared as previously described [35]. |
T4848 | 145-314 | Sentence | denotes | Whole cell lysates were prepared in NP-40 lysis buffer (0.5% Nonidet P-40, 20 mM Tris-HCl [pH 7.5], 150 mM NaCl) in the presence of complete protease cocktail inhibitor. |
T4849 | 315-430 | Sentence | denotes | Lysates were centrifuged at 4°C for 10 min at 12,000 rpm in an Eppendorf microcentrifuge to remove cellular debris. |
T4850 | 431-682 | Sentence | denotes | Samples were then immunoprecipitated with either 10 µl of antibody against GATA-3 or importin-α using A/G agarose slurry in the presence of protease inhibitor using the Catch and Release methodology (Upstate Biotechnology, Lake Placid, New York, USA). |
T4851 | 683-819 | Sentence | denotes | Western blot analysis was performed using anti-GATA-3, anti-importin-α, anti-GR, anti-p-p38 MAP kinase, anti-p-ATF-2, and anti-p-serine. |
T4852 | 820-925 | Sentence | denotes | Immunoreactive proteins were detected using an enhanced chemiluminescence ECL kit (Amersham Biosciences). |
T102 | 0-66 | Sentence | denotes | Cell Fractionation, Immunoprecipitation, and Western Blot Analysis |
T103 | 67-144 | Sentence | denotes | Nuclear and cytoplasmic fractions were prepared as previously described [35]. |
T104 | 145-314 | Sentence | denotes | Whole cell lysates were prepared in NP-40 lysis buffer (0.5% Nonidet P-40, 20 mM Tris-HCl [pH 7.5], 150 mM NaCl) in the presence of complete protease cocktail inhibitor. |
T105 | 315-430 | Sentence | denotes | Lysates were centrifuged at 4°C for 10 min at 12,000 rpm in an Eppendorf microcentrifuge to remove cellular debris. |
T106 | 431-682 | Sentence | denotes | Samples were then immunoprecipitated with either 10 µl of antibody against GATA-3 or importin-α using A/G agarose slurry in the presence of protease inhibitor using the Catch and Release methodology (Upstate Biotechnology, Lake Placid, New York, USA). |
T107 | 683-819 | Sentence | denotes | Western blot analysis was performed using anti-GATA-3, anti-importin-α, anti-GR, anti-p-p38 MAP kinase, anti-p-ATF-2, and anti-p-serine. |
T108 | 820-925 | Sentence | denotes | Immunoreactive proteins were detected using an enhanced chemiluminescence ECL kit (Amersham Biosciences). |
events-check-again
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5188 | 506-512 | Protein | denotes | GATA-3 |
bionlp-st-ge-2016-reference-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5189 | 277-294 | Protein | denotes | complete protease |
T5190 | 304-313 | Negative_regulation | denotes | inhibitor |
T5191 | 506-512 | Protein | denotes | GATA-3 |
T5192 | 516-526 | Protein | denotes | importin-α |
T5193 | 725-736 | Protein | denotes | anti-GATA-3 |
T5194 | 738-753 | Protein | denotes | anti-importin-α |
T5195 | 755-762 | Protein | denotes | anti-GR |
T5196 | 764-774 | Protein | denotes | anti-p-p38 |
T5197 | 775-785 | Protein | denotes | MAP kinase |
T5198 | 719-724 | Positive_regulation | denotes | using |
R4481 | T5189 | T5190 | themeOf | complete protease,inhibitor |
R4482 | T5196 | T5198 | themeOf | anti-p-p38,using |
bionlp-st-ge-2016-reference
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T4845 | 506-512 | Protein | denotes | GATA-3 |
bionlp-st-ge-2016-uniprot
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T5004 | 236-238 | P0A7Z4 | denotes | pH |
T5005 | 506-512 | P23771 | denotes | GATA-3 |
T5006 | 730-736 | P23771 | denotes | GATA-3 |
T5007 | 760-762 | P04150 | denotes | GR |
T5008 | 771-774 | Q16539 | denotes | p38 |
T5009 | 771-774 | Q15759 | denotes | p38 |
T5010 | 771-774 | P53778 | denotes | p38 |
T5011 | 771-774 | O15264 | denotes | p38 |
T5012 | 794-799 | P15336 | denotes | ATF-2 |
test2
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T4844 | 506-512 | Protein | denotes | GATA-3 |