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PMC:2674207 / 15127-16052 JSONTXT

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2_test

Id Subject Object Predicate Lexical cue
19436703-11395507-86274489 140-142 11395507 denotes 35
T8362 140-142 11395507 denotes 35

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4853 0-4 NNP denotes Cell
T4854 5-18 NNP denotes Fractionation
T4855 18-19 -COMMA- denotes ,
T4856 20-39 NN denotes Immunoprecipitation
T4857 39-40 -COMMA- denotes ,
T4858 41-44 CC denotes and
T4859 45-52 NNP denotes Western
T4860 53-57 NNP denotes Blot
T4861 58-66 NN denotes Analysis
T4862 67-74 JJ denotes Nuclear
T4863 75-78 CC denotes and
T4864 79-90 JJ denotes cytoplasmic
T4865 91-100 NN denotes fractions
T4866 101-105 VB denotes were
T4867 106-114 VB denotes prepared
T4868 115-117 IN denotes as
T4869 118-128 RB denotes previously
T4870 129-138 VB denotes described
T4871 139-140 -LRB- denotes [
T4872 140-142 CD denotes 35
T4873 142-143 -RRB- denotes ]
T4874 145-150 JJ denotes Whole
T4875 151-155 NN denotes cell
T4876 156-163 NN denotes lysates
T4877 164-168 VB denotes were
T4878 169-177 VB denotes prepared
T4879 178-180 IN denotes in
T4880 181-186 NN denotes NP-40
T4881 187-192 NN denotes lysis
T4882 193-199 NN denotes buffer
T4883 200-201 -LRB- denotes (
T4884 201-204 CD denotes 0.5
T4885 204-205 NN denotes %
T4886 206-213 NN denotes Nonidet
T4887 214-218 NN denotes P-40
T4888 218-219 -COMMA- denotes ,
T4889 220-222 CD denotes 20
T4890 223-225 NN denotes mM
T4891 226-234 NN denotes Tris-HCl
T4892 235-236 -LRB- denotes [
T4893 236-238 NN denotes pH
T4894 239-242 CD denotes 7.5
T4895 242-243 -RRB- denotes ]
T4896 243-244 -COMMA- denotes ,
T4897 245-248 CD denotes 150
T4898 249-251 NN denotes mM
T4899 252-256 NN denotes NaCl
T4900 256-257 -RRB- denotes )
T4901 258-260 IN denotes in
T4902 261-264 DT denotes the
T4903 265-273 NN denotes presence
T4904 274-276 IN denotes of
T4905 277-285 JJ denotes complete
T4906 286-294 NN denotes protease
T4907 295-303 NN denotes cocktail
T4908 304-313 NN denotes inhibitor
T4909 315-322 NN denotes Lysates
T4910 323-327 VB denotes were
T4911 328-339 VB denotes centrifuged
T4912 340-342 IN denotes at
T4913 343-346 NN denotes 4°C
T4914 347-350 IN denotes for
T4915 351-353 CD denotes 10
T4916 354-357 NN denotes min
T4917 358-360 IN denotes at
T4918 361-367 CD denotes 12,000
T4919 368-371 NN denotes rpm
T4920 372-374 IN denotes in
T4921 375-377 DT denotes an
T4922 378-387 NNP denotes Eppendorf
T4923 388-403 NN denotes microcentrifuge
T4924 404-406 TO denotes to
T4925 407-413 VB denotes remove
T4926 414-422 JJ denotes cellular
T4927 423-429 NN denotes debris
T4928 431-438 NN denotes Samples
T4929 439-443 VB denotes were
T4930 444-448 RB denotes then
T4931 449-467 VB denotes immunoprecipitated
T4932 468-472 IN denotes with
T4933 473-479 CC denotes either
T4934 480-482 CD denotes 10
T4935 483-485 NN denotes µl
T4936 486-488 IN denotes of
T4937 489-497 NN denotes antibody
T4938 498-505 IN denotes against
T4939 506-512 NN denotes GATA-3
T4940 513-515 CC denotes or
T4941 516-526 NN denotes importin-α
T4942 527-532 VB denotes using
T4943 533-536 NN denotes A/G
T4944 537-544 VB denotes agarose
T4945 545-551 NN denotes slurry
T4946 552-554 IN denotes in
T4947 555-558 DT denotes the
T4948 559-567 NN denotes presence
T4949 568-570 IN denotes of
T4950 571-579 NN denotes protease
T4951 580-589 NN denotes inhibitor
T4952 590-595 VB denotes using
T4953 596-599 DT denotes the
T4954 600-605 NNP denotes Catch
T4955 606-609 CC denotes and
T4956 610-617 NN denotes Release
T4957 618-629 NN denotes methodology
T4958 630-631 -LRB- denotes (
T4959 631-638 NNP denotes Upstate
T4960 639-652 NNP denotes Biotechnology
T4961 652-653 -COMMA- denotes ,
T4962 654-658 NNP denotes Lake
T4963 659-665 NNP denotes Placid
T4964 665-666 -COMMA- denotes ,
T4965 667-670 NNP denotes New
T4966 671-675 NNP denotes York
T4967 675-676 -COMMA- denotes ,
T4968 677-680 NNP denotes USA
T4969 680-681 -RRB- denotes )
T4970 683-690 NN denotes Western
T4971 691-695 NN denotes blot
T4972 696-704 NN denotes analysis
T4973 705-708 VB denotes was
T4974 709-718 VB denotes performed
T4975 719-724 VB denotes using
T4976 725-736 NN denotes anti-GATA-3
T4977 736-737 -COMMA- denotes ,
T4978 738-753 JJ denotes anti-importin-α
T4979 753-754 -COMMA- denotes ,
T4980 755-762 JJ denotes anti-GR
T4981 762-763 -COMMA- denotes ,
T4982 764-774 JJ denotes anti-p-p38
T4983 775-778 NN denotes MAP
T4984 779-785 NN denotes kinase
T4985 785-786 -COMMA- denotes ,
T4986 787-799 NN denotes anti-p-ATF-2
T4987 799-800 -COMMA- denotes ,
T4988 801-804 CC denotes and
T4989 805-818 JJ denotes anti-p-serine
T4990 820-834 JJ denotes Immunoreactive
T4991 835-843 NN denotes proteins
T4992 844-848 VB denotes were
T4993 849-857 VB denotes detected
T4994 858-863 VB denotes using
T4995 864-866 DT denotes an
T4996 867-875 VB denotes enhanced
T4997 876-893 NN denotes chemiluminescence
T4998 894-897 NN denotes ECL
T4999 898-901 NN denotes kit
T5000 902-903 -LRB- denotes (
T5001 903-911 NNP denotes Amersham
T5002 912-923 NNP denotes Biosciences
T5003 923-924 -RRB- denotes )
R4166 T4854 T4853 arg1Of Fractionation,Cell
R4167 T4854 T4855 arg1Of Fractionation,","
R4168 T4855 T4858 arg1Of ",",and
R4169 T4856 T4855 arg2Of Immunoprecipitation,","
R4170 T4858 T4857 arg1Of and,","
R4171 T4860 T4859 arg1Of Blot,Western
R4172 T4861 T4858 arg2Of Analysis,and
R4173 T4861 T4860 arg1Of Analysis,Blot
R4174 T4862 T4863 arg1Of Nuclear,and
R4175 T4864 T4863 arg2Of cytoplasmic,and
R4176 T4865 T4862 arg1Of fractions,Nuclear
R4177 T4865 T4864 arg1Of fractions,cytoplasmic
R4178 T4865 T4866 arg1Of fractions,were
R4179 T4865 T4867 arg2Of fractions,prepared
R4180 T4867 T4866 arg2Of prepared,were
R4181 T4867 T4868 arg1Of prepared,as
R4182 T4870 T4869 arg1Of described,previously
R4183 T4872 T4868 arg2Of 35,as
R4184 T4872 T4870 arg1Of 35,described
R4185 T4872 T4871 arg2Of 35,[
R4186 T4873 T4871 arg3Of ],[
R4187 T4876 T4874 arg1Of lysates,Whole
R4188 T4876 T4875 arg1Of lysates,cell
R4189 T4876 T4877 arg1Of lysates,were
R4190 T4876 T4878 arg2Of lysates,prepared
R4191 T4878 T4877 arg2Of prepared,were
R4192 T4878 T4879 arg1Of prepared,in
R4193 T4882 T4879 arg2Of buffer,in
R4194 T4882 T4880 arg1Of buffer,NP-40
R4195 T4882 T4881 arg1Of buffer,lysis
R4196 T4882 T4883 arg1Of buffer,(
R4197 T4882 T4901 arg1Of buffer,in
R4198 T4884 T4885 arg1Of 0.5,%
R4199 T4887 T4884 arg1Of P-40,0.5
R4200 T4887 T4886 arg1Of P-40,Nonidet
R4201 T4887 T4888 arg1Of P-40,","
R4202 T4888 T4896 arg1Of ",",","
R4203 T4889 T4890 arg1Of 20,mM
R4204 T4891 T4888 arg2Of Tris-HCl,","
R4205 T4891 T4889 arg1Of Tris-HCl,20
R4206 T4891 T4892 arg1Of Tris-HCl,[
R4207 T4893 T4892 arg2Of pH,[
R4208 T4893 T4894 arg1Of pH,7.5
R4209 T4895 T4892 arg3Of ],[
R4210 T4896 T4883 arg2Of ",",(
R4211 T4897 T4898 arg1Of 150,mM
R4212 T4899 T4896 arg2Of NaCl,","
R4213 T4899 T4897 arg1Of NaCl,150
R4214 T4900 T4883 arg3Of ),(
R4215 T4903 T4901 arg2Of presence,in
R4216 T4903 T4902 arg1Of presence,the
R4217 T4903 T4904 arg1Of presence,of
R4218 T4908 T4904 arg2Of inhibitor,of
R4219 T4908 T4905 arg1Of inhibitor,complete
R4220 T4908 T4906 arg1Of inhibitor,protease
R4221 T4908 T4907 arg1Of inhibitor,cocktail
R4222 T4909 T4910 arg1Of Lysates,were
R4223 T4909 T4911 arg2Of Lysates,centrifuged
R4224 T4909 T4925 arg1Of Lysates,remove
R4225 T4911 T4910 arg2Of centrifuged,were
R4226 T4911 T4912 arg1Of centrifuged,at
R4227 T4911 T4914 arg1Of centrifuged,for
R4228 T4911 T4917 arg1Of centrifuged,at
R4229 T4911 T4920 arg1Of centrifuged,in
R4230 T4911 T4924 modOf centrifuged,to
R4231 T4913 T4912 arg2Of 4°C,at
R4232 T4916 T4914 arg2Of min,for
R4233 T4916 T4915 arg1Of min,10
R4234 T4919 T4917 arg2Of rpm,at
R4235 T4919 T4918 arg1Of rpm,"12,000"
R4236 T4923 T4920 arg2Of microcentrifuge,in
R4237 T4923 T4921 arg1Of microcentrifuge,an
R4238 T4923 T4922 arg1Of microcentrifuge,Eppendorf
R4239 T4925 T4924 arg1Of remove,to
R4240 T4927 T4925 arg2Of debris,remove
R4241 T4927 T4926 arg1Of debris,cellular
R4242 T4928 T4929 arg1Of Samples,were
R4243 T4928 T4931 arg2Of Samples,immunoprecipitated
R4244 T4931 T4929 arg2Of immunoprecipitated,were
R4245 T4931 T4930 arg1Of immunoprecipitated,then
R4246 T4931 T4932 arg1Of immunoprecipitated,with
R4247 T4935 T4932 arg2Of µl,with
R4248 T4935 T4933 arg1Of µl,either
R4249 T4935 T4934 arg1Of µl,10
R4250 T4935 T4936 arg1Of µl,of
R4251 T4935 T4942 arg1Of µl,using
R4252 T4935 T4952 arg1Of µl,using
R4253 T4935 T4958 arg1Of µl,(
R4254 T4937 T4936 arg2Of antibody,of
R4255 T4937 T4938 arg1Of antibody,against
R4256 T4939 T4940 arg1Of GATA-3,or
R4257 T4940 T4938 arg2Of or,against
R4258 T4941 T4940 arg2Of importin-α,or
R4259 T4942 T4946 arg1Of using,in
R4260 T4942 T4952 modOf using,using
R4261 T4945 T4942 arg2Of slurry,using
R4262 T4945 T4943 arg1Of slurry,A/G
R4263 T4945 T4944 arg2Of slurry,agarose
R4264 T4948 T4946 arg2Of presence,in
R4265 T4948 T4947 arg1Of presence,the
R4266 T4948 T4949 arg1Of presence,of
R4267 T4951 T4949 arg2Of inhibitor,of
R4268 T4951 T4950 arg1Of inhibitor,protease
R4269 T4954 T4955 arg1Of Catch,and
R4270 T4956 T4955 arg2Of Release,and
R4271 T4957 T4952 arg2Of methodology,using
R4272 T4957 T4953 arg1Of methodology,the
R4273 T4957 T4954 arg1Of methodology,Catch
R4274 T4957 T4956 arg1Of methodology,Release
R4275 T4960 T4959 arg1Of Biotechnology,Upstate
R4276 T4960 T4961 arg1Of Biotechnology,","
R4277 T4961 T4964 arg1Of ",",","
R4278 T4963 T4961 arg2Of Placid,","
R4279 T4963 T4962 arg1Of Placid,Lake
R4280 T4964 T4958 arg2Of ",",(
R4281 T4964 T4967 arg1Of ",",","
R4282 T4964 T4968 arg1Of ",",USA
R4283 T4966 T4964 arg2Of York,","
R4284 T4966 T4965 arg1Of York,New
R4285 T4969 T4958 arg3Of ),(
R4286 T4972 T4970 arg1Of analysis,Western
R4287 T4972 T4971 arg1Of analysis,blot
R4288 T4972 T4973 arg1Of analysis,was
R4289 T4972 T4974 arg2Of analysis,performed
R4290 T4974 T4973 arg2Of performed,was
R4291 T4975 T4974 arg3Of using,performed
R4292 T4976 T4975 arg2Of anti-GATA-3,using
R4293 T4976 T4977 arg1Of anti-GATA-3,","
R4294 T4978 T4979 arg1Of anti-importin-α,","
R4295 T4979 T4981 arg1Of ",",","
R4296 T4980 T4979 arg2Of anti-GR,","
R4297 T4982 T4981 arg2Of anti-p-p38,","
R4298 T4984 T4978 arg1Of kinase,anti-importin-α
R4299 T4984 T4980 arg1Of kinase,anti-GR
R4300 T4984 T4982 arg1Of kinase,anti-p-p38
R4301 T4984 T4983 arg1Of kinase,MAP
R4302 T4984 T4985 arg1Of kinase,","
R4303 T4985 T4988 arg1Of ",",and
R4304 T4986 T4985 arg2Of anti-p-ATF-2,","
R4305 T4988 T4977 arg2Of and,","
R4306 T4988 T4987 arg1Of and,","
R4307 T4989 T4988 arg2Of anti-p-serine,and
R4308 T4991 T4990 arg1Of proteins,Immunoreactive
R4309 T4991 T4992 arg1Of proteins,were
R4310 T4991 T4993 arg2Of proteins,detected
R4311 T4993 T4992 arg2Of detected,were
R4312 T4994 T4993 arg3Of using,detected
R4313 T4999 T4994 arg2Of kit,using
R4314 T4999 T4995 arg1Of kit,an
R4315 T4999 T4996 arg2Of kit,enhanced
R4316 T4999 T4997 arg1Of kit,chemiluminescence
R4317 T4999 T4998 arg1Of kit,ECL
R4318 T4999 T5000 arg1Of kit,(
R4319 T5002 T5000 arg2Of Biosciences,(
R4320 T5002 T5001 arg1Of Biosciences,Amersham
R4321 T5003 T5000 arg3Of ),(

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T5013 0-4 NNP denotes Cell
T5014 5-18 NNP denotes Fractionation
T5015 18-19 , denotes ,
T5016 20-39 NNP denotes Immunoprecipitation
T5017 39-40 , denotes ,
T5018 41-44 CC denotes and
T5019 45-52 JJ denotes Western
T5020 53-57 NNP denotes Blot
T5021 58-66 NNP denotes Analysis
T5022 67-74 NNP denotes Nuclear
T5023 75-78 CC denotes and
T5024 79-90 JJ denotes cytoplasmic
T5025 91-100 NNS denotes fractions
T5026 101-105 VBD denotes were
T5027 106-114 VBN denotes prepared
T5028 115-117 IN denotes as
T5029 118-128 RB denotes previously
T5030 129-138 VBN denotes described
T5031 139-140 NNP denotes [
T5032 140-142 CD denotes 35
T5033 142-143 NNP denotes ]
T5034 143-144 . denotes .
T5035 145-150 JJ denotes Whole
T5036 151-155 NN denotes cell
T5037 156-163 NNS denotes lysates
T5038 164-168 VBD denotes were
T5039 169-177 VBN denotes prepared
T5040 178-180 IN denotes in
T5041 181-186 NN denotes NP-40
T5042 187-192 NN denotes lysis
T5043 193-199 NN denotes buffer
T5044 200-201 -LRB- denotes (
T5045 201-204 CD denotes 0.5
T5046 204-205 NN denotes %
T5047 206-213 NNP denotes Nonidet
T5048 214-218 NNP denotes P-40
T5049 218-219 , denotes ,
T5050 220-222 CD denotes 20
T5051 223-225 NNP denotes mM
T5052 226-234 NNP denotes Tris-HCl
T5053 235-236 NNP denotes [
T5054 236-238 NNP denotes pH
T5055 239-242 CD denotes 7.5
T5056 242-243 NNP denotes ]
T5057 243-244 , denotes ,
T5058 245-248 CD denotes 150
T5059 249-251 NNP denotes mM
T5060 252-256 NNP denotes NaCl
T5061 256-257 -RRB- denotes )
T5062 258-260 IN denotes in
T5063 261-264 DT denotes the
T5064 265-273 NN denotes presence
T5065 274-276 IN denotes of
T5066 277-285 JJ denotes complete
T5067 286-294 NN denotes protease
T5068 295-303 NN denotes cocktail
T5069 304-313 NN denotes inhibitor
T5070 313-314 . denotes .
T5071 315-322 NNS denotes Lysates
T5072 323-327 VBD denotes were
T5073 328-339 VBN denotes centrifuged
T5074 340-342 IN denotes at
T5075 343-344 CD denotes 4
T5076 344-345 CD denotes °
T5077 345-346 NNP denotes C
T5078 347-350 IN denotes for
T5079 351-353 CD denotes 10
T5080 354-357 NN denotes min
T5081 358-360 IN denotes at
T5082 361-367 CD denotes 12,000
T5083 368-371 NN denotes rpm
T5084 372-374 IN denotes in
T5085 375-377 DT denotes an
T5086 378-387 NNP denotes Eppendorf
T5087 388-403 NN denotes microcentrifuge
T5088 404-406 TO denotes to
T5089 407-413 VB denotes remove
T5090 414-422 JJ denotes cellular
T5091 423-429 NN denotes debris
T5092 429-430 . denotes .
T5093 431-438 NNS denotes Samples
T5094 439-443 VBD denotes were
T5095 444-448 RB denotes then
T5096 449-467 VBN denotes immunoprecipitated
T5097 468-472 IN denotes with
T5098 473-479 DT denotes either
T5099 480-482 CD denotes 10
T5100 483-485 NN denotes µl
T5101 486-488 IN denotes of
T5102 489-497 NN denotes antibody
T5103 498-505 IN denotes against
T5104 506-512 NNP denotes GATA-3
T5105 513-515 CC denotes or
T5106 516-526 JJ denotes importin-α
T5107 527-532 VBG denotes using
T5108 533-536 NNP denotes A/G
T5109 537-544 JJ denotes agarose
T5110 545-551 NN denotes slurry
T5111 552-554 IN denotes in
T5112 555-558 DT denotes the
T5113 559-567 NN denotes presence
T5114 568-570 IN denotes of
T5115 571-579 NN denotes protease
T5116 580-589 NN denotes inhibitor
T5117 590-595 VBG denotes using
T5118 596-599 DT denotes the
T5119 600-605 NNP denotes Catch
T5120 606-609 CC denotes and
T5121 610-617 NNP denotes Release
T5122 618-629 NN denotes methodology
T5123 630-631 -LRB- denotes (
T5124 631-638 NNP denotes Upstate
T5125 639-652 NNP denotes Biotechnology
T5126 652-653 , denotes ,
T5127 654-658 NNP denotes Lake
T5128 659-665 NNP denotes Placid
T5129 665-666 , denotes ,
T5130 667-670 NNP denotes New
T5131 671-675 NNP denotes York
T5132 675-676 , denotes ,
T5133 677-680 NNP denotes USA
T5134 680-681 -RRB- denotes )
T5135 681-682 . denotes .
T5136 683-690 JJ denotes Western
T5137 691-695 NN denotes blot
T5138 696-704 NN denotes analysis
T5139 705-708 VBD denotes was
T5140 709-718 VBN denotes performed
T5141 719-724 VBG denotes using
T5142 725-736 JJ denotes anti-GATA-3
T5143 736-737 , denotes ,
T5144 738-753 JJ denotes anti-importin-α
T5145 753-754 , denotes ,
T5146 755-762 JJ denotes anti-GR
T5147 762-763 , denotes ,
T5148 764-774 JJ denotes anti-p-p38
T5149 775-778 NNP denotes MAP
T5150 779-785 NN denotes kinase
T5151 785-786 , denotes ,
T5152 787-799 JJ denotes anti-p-ATF-2
T5153 799-800 , denotes ,
T5154 801-804 CC denotes and
T5155 805-818 JJ denotes anti-p-serine
T5156 818-819 . denotes .
T5157 820-834 JJ denotes Immunoreactive
T5158 835-843 NNS denotes proteins
T5159 844-848 VBD denotes were
T5160 849-857 VBN denotes detected
T5161 858-863 VBG denotes using
T5162 864-866 DT denotes an
T5163 867-875 JJ denotes enhanced
T5164 876-893 NN denotes chemiluminescence
T5165 894-897 NNP denotes ECL
T5166 898-901 NN denotes kit
T5167 902-903 -LRB- denotes (
T5168 903-911 NNP denotes Amersham
T5169 912-923 NNPS denotes Biosciences
T5170 923-924 -RRB- denotes )
T5171 924-925 . denotes .
R4322 T5013 T5014 compound Cell,Fractionation
R4323 T5014 T5027 nsubjpass Fractionation,prepared
R4324 T5015 T5014 punct ",",Fractionation
R4325 T5016 T5014 conj Immunoprecipitation,Fractionation
R4326 T5017 T5016 punct ",",Immunoprecipitation
R4327 T5018 T5016 cc and,Immunoprecipitation
R4328 T5019 T5022 amod Western,Nuclear
R4329 T5020 T5022 compound Blot,Nuclear
R4330 T5021 T5022 compound Analysis,Nuclear
R4331 T5022 T5016 conj Nuclear,Immunoprecipitation
R4332 T5023 T5022 cc and,Nuclear
R4333 T5024 T5022 conj cytoplasmic,Nuclear
R4334 T5025 T5014 appos fractions,Fractionation
R4335 T5026 T5027 auxpass were,prepared
R4336 T5027 T5027 ROOT prepared,prepared
R4337 T5028 T5030 mark as,described
R4338 T5029 T5030 advmod previously,described
R4339 T5030 T5027 advcl described,prepared
R4340 T5031 T5033 nmod [,]
R4341 T5032 T5033 nummod 35,]
R4342 T5033 T5030 dobj ],described
R4343 T5034 T5027 punct .,prepared
R4344 T5035 T5037 amod Whole,lysates
R4345 T5036 T5037 compound cell,lysates
R4346 T5037 T5039 nsubjpass lysates,prepared
R4347 T5038 T5039 auxpass were,prepared
R4348 T5039 T5039 ROOT prepared,prepared
R4349 T5040 T5039 prep in,prepared
R4350 T5041 T5043 compound NP-40,buffer
R4351 T5042 T5043 compound lysis,buffer
R4352 T5043 T5040 pobj buffer,in
R4353 T5044 T5048 punct (,P-40
R4354 T5045 T5046 nummod 0.5,%
R4355 T5046 T5048 compound %,P-40
R4356 T5047 T5048 compound Nonidet,P-40
R4357 T5048 T5043 appos P-40,buffer
R4358 T5049 T5048 punct ",",P-40
R4359 T5050 T5054 nummod 20,pH
R4360 T5051 T5054 compound mM,pH
R4361 T5052 T5054 compound Tris-HCl,pH
R4415 T5106 T5104 conj importin-α,GATA-3
R4438 T5129 T5128 punct ",",Placid
R4439 T5130 T5131 compound New,York
R4440 T5131 T5128 conj York,Placid
R4441 T5132 T5131 punct ",",York
R4442 T5133 T5131 conj USA,York
R4443 T5134 T5125 punct ),Biotechnology
R4444 T5135 T5096 punct .,immunoprecipitated
R4445 T5136 T5138 amod Western,analysis
R4446 T5137 T5138 compound blot,analysis
R4447 T5138 T5140 nsubjpass analysis,performed
R4448 T5139 T5140 auxpass was,performed
R4449 T5140 T5140 ROOT performed,performed
R4450 T5141 T5140 advcl using,performed
R4451 T5142 T5141 dobj anti-GATA-3,using
R4452 T5143 T5142 punct ",",anti-GATA-3
R4453 T5144 T5142 conj anti-importin-α,anti-GATA-3
R4454 T5145 T5144 punct ",",anti-importin-α
R4455 T5146 T5144 conj anti-GR,anti-importin-α
R4456 T5147 T5146 punct ",",anti-GR
R4457 T5148 T5150 amod anti-p-p38,kinase
R4458 T5149 T5150 compound MAP,kinase
R4459 T5150 T5146 conj kinase,anti-GR
R4460 T5151 T5150 punct ",",kinase
R4461 T5152 T5150 conj anti-p-ATF-2,kinase
R4462 T5153 T5152 punct ",",anti-p-ATF-2
R4463 T5154 T5152 cc and,anti-p-ATF-2
R4464 T5155 T5152 conj anti-p-serine,anti-p-ATF-2
R4465 T5156 T5140 punct .,performed
R4466 T5157 T5158 amod Immunoreactive,proteins
R4467 T5158 T5160 nsubjpass proteins,detected
R4468 T5159 T5160 auxpass were,detected
R4469 T5160 T5160 ROOT detected,detected
R4470 T5161 T5160 advcl using,detected
R4471 T5162 T5166 det an,kit
R4472 T5163 T5166 amod enhanced,kit
R4473 T5164 T5165 compound chemiluminescence,ECL
R4474 T5165 T5166 compound ECL,kit
R4475 T5166 T5161 dobj kit,using
R4476 T5167 T5166 punct (,kit
R4477 T5168 T5169 compound Amersham,Biosciences
R4478 T5169 T5166 appos Biosciences,kit
R4479 T5170 T5166 punct ),kit
R4480 T5171 T5160 punct .,detected
R4362 T5053 T5054 compound [,pH
R4363 T5054 T5048 appos pH,P-40
R4364 T5055 T5056 nummod 7.5,]
R4365 T5056 T5056 ROOT ],]
R4366 T5057 T5056 punct ",",]
R4367 T5058 T5060 nummod 150,NaCl
R4368 T5059 T5060 compound mM,NaCl
R4369 T5060 T5056 appos NaCl,]
R4370 T5061 T5056 punct ),]
R4371 T5062 T5056 prep in,]
R4372 T5063 T5064 det the,presence
R4373 T5064 T5062 pobj presence,in
R4374 T5065 T5064 prep of,presence
R4375 T5066 T5069 amod complete,inhibitor
R4376 T5067 T5069 compound protease,inhibitor
R4377 T5068 T5069 compound cocktail,inhibitor
R4378 T5069 T5065 pobj inhibitor,of
R4379 T5070 T5039 punct .,prepared
R4380 T5071 T5073 nsubjpass Lysates,centrifuged
R4381 T5072 T5073 auxpass were,centrifuged
R4382 T5073 T5073 ROOT centrifuged,centrifuged
R4383 T5074 T5073 prep at,centrifuged
R4384 T5075 T5076 nummod 4,°
R4385 T5076 T5074 pobj °,at
R4386 T5077 T5073 conj C,centrifuged
R4387 T5078 T5077 prep for,C
R4388 T5079 T5080 nummod 10,min
R4389 T5080 T5078 pobj min,for
R4390 T5081 T5080 prep at,min
R4391 T5082 T5083 nummod "12,000",rpm
R4392 T5083 T5081 pobj rpm,at
R4393 T5084 T5073 prep in,centrifuged
R4394 T5085 T5087 det an,microcentrifuge
R4395 T5086 T5087 compound Eppendorf,microcentrifuge
R4396 T5087 T5084 pobj microcentrifuge,in
R4397 T5088 T5089 aux to,remove
R4398 T5089 T5073 advcl remove,centrifuged
R4399 T5090 T5091 amod cellular,debris
R4400 T5091 T5089 dobj debris,remove
R4401 T5092 T5073 punct .,centrifuged
R4402 T5093 T5096 nsubjpass Samples,immunoprecipitated
R4403 T5094 T5096 auxpass were,immunoprecipitated
R4404 T5095 T5096 advmod then,immunoprecipitated
R4405 T5096 T5096 ROOT immunoprecipitated,immunoprecipitated
R4406 T5097 T5096 prep with,immunoprecipitated
R4407 T5098 T5100 preconj either,µl
R4408 T5099 T5100 nummod 10,µl
R4409 T5100 T5107 nsubj µl,using
R4410 T5101 T5100 prep of,µl
R4411 T5102 T5101 pobj antibody,of
R4412 T5103 T5102 prep against,antibody
R4413 T5104 T5103 pobj GATA-3,against
R4414 T5105 T5104 cc or,GATA-3
R4416 T5107 T5097 pcomp using,with
R4417 T5108 T5110 nmod A/G,slurry
R4418 T5109 T5110 amod agarose,slurry
R4419 T5110 T5107 dobj slurry,using
R4420 T5111 T5107 prep in,using
R4421 T5112 T5113 det the,presence
R4422 T5113 T5111 pobj presence,in
R4423 T5114 T5113 prep of,presence
R4424 T5115 T5116 amod protease,inhibitor
R4425 T5116 T5114 pobj inhibitor,of
R4426 T5117 T5107 advcl using,using
R4427 T5118 T5119 det the,Catch
R4428 T5119 T5117 dobj Catch,using
R4429 T5120 T5119 cc and,Catch
R4430 T5121 T5119 conj Release,Catch
R4431 T5122 T5119 conj methodology,Catch
R4432 T5123 T5125 punct (,Biotechnology
R4433 T5124 T5125 compound Upstate,Biotechnology
R4434 T5125 T5119 conj Biotechnology,Catch
R4435 T5126 T5125 punct ",",Biotechnology
R4436 T5127 T5128 compound Lake,Placid
R4437 T5128 T5125 conj Placid,Biotechnology

GO-BP

Id Subject Object Predicate Lexical cue
T5179 187-192 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T5180 414-422 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular

GO-MF

Id Subject Object Predicate Lexical cue
T5181 489-497 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody

GO-CC

Id Subject Object Predicate Lexical cue
T5182 0-4 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T5183 151-155 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T5184 489-497 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T5185 489-497 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

sentences

Id Subject Object Predicate Lexical cue
T4846 0-66 Sentence denotes Cell Fractionation, Immunoprecipitation, and Western Blot Analysis
T4847 67-144 Sentence denotes Nuclear and cytoplasmic fractions were prepared as previously described [35].
T4848 145-314 Sentence denotes Whole cell lysates were prepared in NP-40 lysis buffer (0.5% Nonidet P-40, 20 mM Tris-HCl [pH 7.5], 150 mM NaCl) in the presence of complete protease cocktail inhibitor.
T4849 315-430 Sentence denotes Lysates were centrifuged at 4°C for 10 min at 12,000 rpm in an Eppendorf microcentrifuge to remove cellular debris.
T4850 431-682 Sentence denotes Samples were then immunoprecipitated with either 10 µl of antibody against GATA-3 or importin-α using A/G agarose slurry in the presence of protease inhibitor using the Catch and Release methodology (Upstate Biotechnology, Lake Placid, New York, USA).
T4851 683-819 Sentence denotes Western blot analysis was performed using anti-GATA-3, anti-importin-α, anti-GR, anti-p-p38 MAP kinase, anti-p-ATF-2, and anti-p-serine.
T4852 820-925 Sentence denotes Immunoreactive proteins were detected using an enhanced chemiluminescence ECL kit (Amersham Biosciences).
T102 0-66 Sentence denotes Cell Fractionation, Immunoprecipitation, and Western Blot Analysis
T103 67-144 Sentence denotes Nuclear and cytoplasmic fractions were prepared as previously described [35].
T104 145-314 Sentence denotes Whole cell lysates were prepared in NP-40 lysis buffer (0.5% Nonidet P-40, 20 mM Tris-HCl [pH 7.5], 150 mM NaCl) in the presence of complete protease cocktail inhibitor.
T105 315-430 Sentence denotes Lysates were centrifuged at 4°C for 10 min at 12,000 rpm in an Eppendorf microcentrifuge to remove cellular debris.
T106 431-682 Sentence denotes Samples were then immunoprecipitated with either 10 µl of antibody against GATA-3 or importin-α using A/G agarose slurry in the presence of protease inhibitor using the Catch and Release methodology (Upstate Biotechnology, Lake Placid, New York, USA).
T107 683-819 Sentence denotes Western blot analysis was performed using anti-GATA-3, anti-importin-α, anti-GR, anti-p-p38 MAP kinase, anti-p-ATF-2, and anti-p-serine.
T108 820-925 Sentence denotes Immunoreactive proteins were detected using an enhanced chemiluminescence ECL kit (Amersham Biosciences).

events-check-again

Id Subject Object Predicate Lexical cue
T5188 506-512 Protein denotes GATA-3

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T5189 277-294 Protein denotes complete protease
T5190 304-313 Negative_regulation denotes inhibitor
T5191 506-512 Protein denotes GATA-3
T5192 516-526 Protein denotes importin-α
T5193 725-736 Protein denotes anti-GATA-3
T5194 738-753 Protein denotes anti-importin-α
T5195 755-762 Protein denotes anti-GR
T5196 764-774 Protein denotes anti-p-p38
T5197 775-785 Protein denotes MAP kinase
T5198 719-724 Positive_regulation denotes using
R4481 T5189 T5190 themeOf complete protease,inhibitor
R4482 T5196 T5198 themeOf anti-p-p38,using

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T4845 506-512 Protein denotes GATA-3

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T5004 236-238 P0A7Z4 denotes pH
T5005 506-512 P23771 denotes GATA-3
T5006 730-736 P23771 denotes GATA-3
T5007 760-762 P04150 denotes GR
T5008 771-774 Q16539 denotes p38
T5009 771-774 Q15759 denotes p38
T5010 771-774 P53778 denotes p38
T5011 771-774 O15264 denotes p38
T5012 794-799 P15336 denotes ATF-2

test2

Id Subject Object Predicate Lexical cue
T4844 506-512 Protein denotes GATA-3