PMC:2674207 / 13960-14564 JSON TXT

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2_test

{"project":"2_test","denotations":[{"id":"19436703-17277157-86274486","span":{"begin":177,"end":179},"obj":"17277157"},{"id":"19436703-15860753-86274487","span":{"begin":331,"end":333},"obj":"15860753"},{"id":"19436703-17277157-86274488","span":{"begin":600,"end":602},"obj":"17277157"},{"id":"T38919","span":{"begin":177,"end":179},"obj":"17277157"},{"id":"T24071","span":{"begin":331,"end":333},"obj":"15860753"},{"id":"T69573","span":{"begin":600,"end":602},"obj":"17277157"}],"text":"Cell Culture and PBMC Isolation\nA human T cell line (HuT-78) was purchased from ECACC European Collection of Cell Culture (Wiltshire, UK) were cultured as previously described [12]. PBMCs were isolated by density centrifugation over Ficoll-Hypaque (density, 1.077 g/ml; Amersham Biosciences, Amersham, UK) as previously described [34]. Cells were stimulated with anti-CD3/CD28 (1 µg/ml each) for 1 h at 37°C to stimulate Th2 cytokine release in the presence or absence of FP (10−12 to 10−8M). Cytospins were prepared and GATA-3 localization determined by confocal microscopy as previously described [12]."}

pmc-enju-pas

bionlp-st-ge-2016-spacy-parsed

GO-BP

{"project":"GO-BP","denotations":[{"id":"T4599","span":{"begin":528,"end":540},"obj":"http://purl.obolibrary.org/obo/GO_0051179"}],"text":"Cell Culture and PBMC Isolation\nA human T cell line (HuT-78) was purchased from ECACC European Collection of Cell Culture (Wiltshire, UK) were cultured as previously described [12]. PBMCs were isolated by density centrifugation over Ficoll-Hypaque (density, 1.077 g/ml; Amersham Biosciences, Amersham, UK) as previously described [34]. Cells were stimulated with anti-CD3/CD28 (1 µg/ml each) for 1 h at 37°C to stimulate Th2 cytokine release in the presence or absence of FP (10−12 to 10−8M). Cytospins were prepared and GATA-3 localization determined by confocal microscopy as previously described [12]."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T4600","span":{"begin":0,"end":4},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4601","span":{"begin":109,"end":113},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Cell Culture and PBMC Isolation\nA human T cell line (HuT-78) was purchased from ECACC European Collection of Cell Culture (Wiltshire, UK) were cultured as previously described [12]. PBMCs were isolated by density centrifugation over Ficoll-Hypaque (density, 1.077 g/ml; Amersham Biosciences, Amersham, UK) as previously described [34]. Cells were stimulated with anti-CD3/CD28 (1 µg/ml each) for 1 h at 37°C to stimulate Th2 cytokine release in the presence or absence of FP (10−12 to 10−8M). Cytospins were prepared and GATA-3 localization determined by confocal microscopy as previously described [12]."}

sentences

{"project":"sentences","denotations":[{"id":"T4349","span":{"begin":0,"end":31},"obj":"Sentence"},{"id":"T4350","span":{"begin":32,"end":181},"obj":"Sentence"},{"id":"T4351","span":{"begin":182,"end":335},"obj":"Sentence"},{"id":"T4352","span":{"begin":336,"end":492},"obj":"Sentence"},{"id":"T4353","span":{"begin":493,"end":604},"obj":"Sentence"},{"id":"T90","span":{"begin":0,"end":31},"obj":"Sentence"},{"id":"T91","span":{"begin":32,"end":181},"obj":"Sentence"},{"id":"T92","span":{"begin":182,"end":335},"obj":"Sentence"},{"id":"T93","span":{"begin":336,"end":492},"obj":"Sentence"},{"id":"T94","span":{"begin":493,"end":604},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Cell Culture and PBMC Isolation\nA human T cell line (HuT-78) was purchased from ECACC European Collection of Cell Culture (Wiltshire, UK) were cultured as previously described [12]. PBMCs were isolated by density centrifugation over Ficoll-Hypaque (density, 1.077 g/ml; Amersham Biosciences, Amersham, UK) as previously described [34]. Cells were stimulated with anti-CD3/CD28 (1 µg/ml each) for 1 h at 37°C to stimulate Th2 cytokine release in the presence or absence of FP (10−12 to 10−8M). Cytospins were prepared and GATA-3 localization determined by confocal microscopy as previously described [12]."}

events-check-again

{"project":"events-check-again","denotations":[{"id":"T4606","span":{"begin":521,"end":527},"obj":"Protein"},{"id":"T4607","span":{"begin":528,"end":540},"obj":"Localization"}],"relations":[{"id":"R3967","pred":"themeOf","subj":"T4606","obj":"T4607"}],"text":"Cell Culture and PBMC Isolation\nA human T cell line (HuT-78) was purchased from ECACC European Collection of Cell Culture (Wiltshire, UK) were cultured as previously described [12]. PBMCs were isolated by density centrifugation over Ficoll-Hypaque (density, 1.077 g/ml; Amersham Biosciences, Amersham, UK) as previously described [34]. Cells were stimulated with anti-CD3/CD28 (1 µg/ml each) for 1 h at 37°C to stimulate Th2 cytokine release in the presence or absence of FP (10−12 to 10−8M). Cytospins were prepared and GATA-3 localization determined by confocal microscopy as previously described [12]."}

bionlp-st-ge-2016-reference-tees

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T4610","span":{"begin":421,"end":441},"obj":"Protein"},{"id":"T4608","span":{"begin":363,"end":371},"obj":"Protein"},{"id":"T4609","span":{"begin":372,"end":376},"obj":"Protein"},{"id":"T4611","span":{"begin":411,"end":420},"obj":"Positive_regulation"}],"relations":[{"id":"R3968","pred":"themeOf","subj":"T4610","obj":"T4611"}],"text":"Cell Culture and PBMC Isolation\nA human T cell line (HuT-78) was purchased from ECACC European Collection of Cell Culture (Wiltshire, UK) were cultured as previously described [12]. PBMCs were isolated by density centrifugation over Ficoll-Hypaque (density, 1.077 g/ml; Amersham Biosciences, Amersham, UK) as previously described [34]. Cells were stimulated with anti-CD3/CD28 (1 µg/ml each) for 1 h at 37°C to stimulate Th2 cytokine release in the presence or absence of FP (10−12 to 10−8M). Cytospins were prepared and GATA-3 localization determined by confocal microscopy as previously described [12]."}

bionlp-st-ge-2016-reference

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T4347","span":{"begin":521,"end":527},"obj":"Protein"},{"id":"T4348","span":{"begin":528,"end":540},"obj":"Localization"}],"relations":[{"id":"R3730","pred":"themeOf","subj":"T4347","obj":"T4348"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Cell Culture and PBMC Isolation\nA human T cell line (HuT-78) was purchased from ECACC European Collection of Cell Culture (Wiltshire, UK) were cultured as previously described [12]. PBMCs were isolated by density centrifugation over Ficoll-Hypaque (density, 1.077 g/ml; Amersham Biosciences, Amersham, UK) as previously described [34]. Cells were stimulated with anti-CD3/CD28 (1 µg/ml each) for 1 h at 37°C to stimulate Th2 cytokine release in the presence or absence of FP (10−12 to 10−8M). Cytospins were prepared and GATA-3 localization determined by confocal microscopy as previously described [12]."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T4464","span":{"begin":368,"end":371},"obj":"P04234"},{"id":"T4465","span":{"begin":368,"end":371},"obj":"P20963"},{"id":"T4466","span":{"begin":368,"end":371},"obj":"P09693"},{"id":"T4467","span":{"begin":368,"end":371},"obj":"P07766"},{"id":"T4468","span":{"begin":372,"end":376},"obj":"P10747"},{"id":"T4469","span":{"begin":521,"end":527},"obj":"P23771"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Cell Culture and PBMC Isolation\nA human T cell line (HuT-78) was purchased from ECACC European Collection of Cell Culture (Wiltshire, UK) were cultured as previously described [12]. PBMCs were isolated by density centrifugation over Ficoll-Hypaque (density, 1.077 g/ml; Amersham Biosciences, Amersham, UK) as previously described [34]. Cells were stimulated with anti-CD3/CD28 (1 µg/ml each) for 1 h at 37°C to stimulate Th2 cytokine release in the presence or absence of FP (10−12 to 10−8M). Cytospins were prepared and GATA-3 localization determined by confocal microscopy as previously described [12]."}

test2

{"project":"test2","denotations":[{"id":"T4345","span":{"begin":521,"end":527},"obj":"Protein"},{"id":"T4346","span":{"begin":528,"end":540},"obj":"Localization"}],"relations":[{"id":"R3729","pred":"themeOf","subj":"T4345","obj":"T4346"}],"text":"Cell Culture and PBMC Isolation\nA human T cell line (HuT-78) was purchased from ECACC European Collection of Cell Culture (Wiltshire, UK) were cultured as previously described [12]. PBMCs were isolated by density centrifugation over Ficoll-Hypaque (density, 1.077 g/ml; Amersham Biosciences, Amersham, UK) as previously described [34]. Cells were stimulated with anti-CD3/CD28 (1 µg/ml each) for 1 h at 37°C to stimulate Th2 cytokine release in the presence or absence of FP (10−12 to 10−8M). Cytospins were prepared and GATA-3 localization determined by confocal microscopy as previously described [12]."}

PMC:2674207 / 13960-14564 JSONTXT

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PMC:2674207 / 13960-14564 JSON TXT