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2_test

Id Subject Object Predicate Lexical cue
19436703-15860753-86274485 246-248 15860753 denotes 34
19436703-17277157-86274486 1810-1812 17277157 denotes 12
19436703-15860753-86274487 1964-1966 15860753 denotes 34
19436703-17277157-86274488 2233-2235 17277157 denotes 12
19436703-11395507-86274489 2940-2942 11395507 denotes 35
19436703-17277157-86274490 3831-3833 17277157 denotes 12
19436703-10958685-86274491 4595-4597 10958685 denotes 36
19436703-15860753-86274492 6282-6284 15860753 denotes 34
T86367 246-248 15860753 denotes 34
T38919 1810-1812 17277157 denotes 12
T24071 1964-1966 15860753 denotes 34
T69573 2233-2235 17277157 denotes 12
T8362 2940-2942 11395507 denotes 35
T57036 3831-3833 17277157 denotes 12
T21764 4595-4597 10958685 denotes 36
T87031 6282-6284 15860753 denotes 34

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T3675 23-35 NN denotes Participants
T3676 36-39 CC denotes and
T3677 40-45 NN denotes Study
T3678 46-52 NN denotes Design
T3679 53-55 PRP denotes We
T3680 56-63 VB denotes studied
T3681 64-72 NN denotes patients
T3682 73-77 IN denotes with
T3683 78-82 JJ denotes mild
T3684 83-89 NN denotes asthma
T3685 90-93 WP denotes who
T3686 94-98 VB denotes were
T3687 99-102 RB denotes not
T3688 103-110 VB denotes treated
T3689 111-115 IN denotes with
T3690 116-123 VB denotes inhaled
T3691 124-139 NN denotes corticosteroids
T3692 140-143 WP denotes who
T3693 144-147 VB denotes had
T3694 148-152 VB denotes been
T3695 153-161 VB denotes included
T3696 162-164 IN denotes in
T3697 165-166 DT denotes a
T3698 167-177 RB denotes previously
T3699 178-186 VB denotes reported
T3700 187-199 JJ denotes double-blind
T3701 199-200 -COMMA- denotes ,
T3702 201-219 JJ denotes placebo-controlled
T3703 219-220 -COMMA- denotes ,
T3704 221-230 JJ denotes crossover
T3705 231-236 NN denotes study
T3706 237-241 IN denotes with
T3707 242-244 NN denotes FP
T3708 245-246 -LRB- denotes [
T3709 246-248 CD denotes 34
T3710 248-249 -RRB- denotes ]
T3711 251-256 CD denotes Seven
T3712 257-265 NN denotes patients
T3713 266-270 IN denotes with
T3714 271-275 JJ denotes mild
T3715 276-282 NN denotes asthma
T3716 283-290 VB denotes entered
T3717 291-294 DT denotes the
T3718 295-300 NN denotes study
T3719 301-304 CC denotes and
T3720 305-309 VB denotes were
T3721 310-320 VB denotes randomized
T3722 321-323 TO denotes to
T3723 324-331 VB denotes receive
T3724 332-333 DT denotes a
T3725 334-340 JJ denotes single
T3726 341-351 NN denotes inhalation
T3727 352-354 IN denotes of
T3728 355-357 NN denotes FP
T3729 358-359 -LRB- denotes (
T3730 359-362 CD denotes 100
T3731 363-366 CC denotes and
T3732 367-370 CD denotes 500
T3733 371-373 NN denotes µg
T3734 373-374 -RRB- denotes )
T3735 375-377 CC denotes or
T3736 378-379 DT denotes a
T3737 380-387 JJ denotes matched
T3738 388-395 NN denotes placebo
T3739 396-403 NN denotes control
T3740 404-407 IN denotes via
T3741 408-409 DT denotes a
T3742 410-416 NN denotes spacer
T3743 417-424 NN denotes chamber
T3744 424-425 -COMMA- denotes ,
T3745 426-429 CC denotes and
T3746 430-433 DT denotes the
T3747 434-439 JJ denotes other
T3748 440-449 NN denotes treatment
T3749 450-453 VB denotes was
T3750 454-459 VB denotes given
T3751 460-465 IN denotes after
T3752 466-467 DT denotes a
T3753 468-476 JJ denotes wash-out
T3754 477-483 NN denotes period
T3755 484-486 IN denotes of
T3756 487-489 IN denotes at
T3757 490-495 JJ denotes least
T3758 496-497 CD denotes 6
T3759 498-499 NN denotes d
T3760 501-506 NN denotes Blood
T3761 507-510 VB denotes was
T3762 511-516 VB denotes taken
T3763 517-520 IN denotes for
T3764 521-532 NN denotes preparation
T3765 533-535 IN denotes of
T3766 536-541 NN denotes PBMCs
T3767 542-544 IN denotes at
T3768 545-546 CD denotes 1
T3769 547-550 CC denotes and
T3770 551-552 CD denotes 2
T3771 553-554 NN denotes h
T3772 555-560 IN denotes after
T3773 561-565 NN denotes drug
T3774 566-580 NN denotes administration
T3775 582-585 DT denotes All
T3776 586-594 NN denotes patients
T3777 595-599 VB denotes gave
T3778 600-608 VB denotes informed
T3779 609-616 NN denotes consent
T3780 617-620 CC denotes and
T3781 621-624 DT denotes the
T3782 625-630 NN denotes study
T3783 631-634 VB denotes was
T3784 635-643 VB denotes approved
T3785 644-646 IN denotes by
T3786 647-650 DT denotes the
T3787 651-657 NNP denotes Ethics
T3788 658-667 NNP denotes Committee
T3789 668-670 IN denotes of
T3790 671-674 DT denotes the
T3791 675-680 NNP denotes Royal
T3792 681-689 NNP denotes Brompton
T3793 690-693 CC denotes and
T3794 694-703 NNP denotes Harefield
T3795 704-713 NNP denotes Hospitals
T3796 714-717 NNP denotes NHS
T3797 718-724 NNP denotes Trust.
T3798 725-728 DT denotes The
T3799 729-737 JJ denotes clinical
T3800 738-743 NN denotes study
T3801 744-747 VB denotes was
T3802 748-757 VB denotes conducted
T3803 758-764 IN denotes before
T3804 765-768 DT denotes the
T3805 769-780 NN denotes requirement
T3806 781-784 IN denotes for
T3807 785-793 NNP denotes Clinical
T3808 794-799 NNP denotes Trial
T3809 800-812 NNP denotes Registration
T3981 815-825 NN denotes Antibodies
T3982 826-829 CC denotes and
T3983 830-838 NN denotes Reagents
T3984 839-842 DT denotes The
T3985 843-853 JJ denotes monoclonal
T3986 854-864 NN denotes antibodies
T3987 865-872 IN denotes against
T3988 873-878 JJ denotes human
T3989 879-882 NN denotes CD3
T3990 882-883 -COMMA- denotes ,
T3991 884-888 NN denotes CD28
T3992 888-889 -COMMA- denotes ,
T3993 890-892 NN denotes GR
T3994 892-893 -COMMA- denotes ,
T3995 894-897 CC denotes and
T3996 898-908 NN denotes importin-α
T3997 909-913 VB denotes were
T3998 914-923 VB denotes purchased
T3999 924-928 IN denotes from
T4000 929-931 NN denotes BD
T4001 932-943 NNP denotes Biosciences
T4002 944-945 -LRB- denotes (
T4003 945-951 NNP denotes Oxford
T4004 951-952 -COMMA- denotes ,
T4005 953-959 NNP denotes United
T4006 960-967 NNP denotes Kingdom
T4007 967-968 -RRB- denotes )
T4008 970-976 NN denotes Rabbit
T4009 977-987 NN denotes antibodies
T4010 988-995 IN denotes against
T4011 996-1001 JJ denotes human
T4012 1002-1008 NN denotes GATA-3
T4013 1009-1010 -LRB- denotes (
T4014 1010-1014 NN denotes H-48
T4015 1014-1015 -RRB- denotes )
T4016 1016-1019 CC denotes and
T4017 1020-1022 NN denotes GR
T4018 1023-1024 -LRB- denotes (
T4019 1024-1028 NN denotes E-20
T4020 1028-1029 -COMMA- denotes ,
T4021 1030-1037 NN denotes sc-1003
T4022 1037-1038 -RRB- denotes )
T4023 1039-1043 VB denotes were
T4024 1044-1052 VB denotes obtained
T4025 1053-1057 IN denotes from
T4026 1058-1063 NNP denotes Santa
T4027 1064-1068 NNP denotes Cruz
T4028 1069-1082 NNP denotes Biotechnology
T4029 1083-1084 -LRB- denotes (
T4030 1084-1089 NNP denotes Santa
T4031 1090-1094 NNP denotes Cruz
T4032 1094-1095 -COMMA- denotes ,
T4033 1096-1106 NNP denotes California
T4034 1106-1107 -COMMA- denotes ,
T4035 1108-1114 NNP denotes United
T4036 1115-1121 NNP denotes States
T4037 1121-1122 -RRB- denotes )
T4038 1122-1123 -COMMA- denotes ,
T4039 1124-1134 JJ denotes polyclonal
T4040 1135-1141 NN denotes rabbit
T4041 1142-1152 NN denotes antibodies
T4042 1153-1160 IN denotes against
T4043 1161-1172 JJ denotes phospho-p38
T4044 1173-1176 NN denotes MAP
T4045 1177-1183 NN denotes kinase
T4046 1184-1187 CC denotes and
T4047 1188-1201 NN denotes phospho-ATF-2
T4048 1202-1206 IN denotes from
T4049 1207-1211 NNP denotes Cell
T4050 1212-1221 NNP denotes Signaling
T4051 1222-1232 NNP denotes Technology
T4052 1233-1234 -LRB- denotes (
T4053 1234-1237 NNP denotes New
T4054 1238-1245 NNP denotes England
T4055 1246-1253 NNP denotes Biolabs
T4056 1253-1254 -COMMA- denotes ,
T4057 1255-1263 NNP denotes Hertford
T4058 1263-1264 -COMMA- denotes ,
T4059 1265-1267 NNP denotes UK
T4060 1267-1268 -RRB- denotes )
T4061 1268-1269 -COMMA- denotes ,
T4062 1270-1280 JJ denotes monoclonal
T4063 1281-1289 NN denotes antibody
T4064 1290-1297 IN denotes against
T4065 1298-1311 NN denotes phosphoserine
T4066 1312-1313 -LRB- denotes (
T4067 1313-1318 NN denotes clone
T4068 1319-1322 NN denotes 4H4
T4069 1322-1323 -RRB- denotes )
T4070 1324-1328 IN denotes from
T4071 1329-1337 NNP denotes Affiniti
T4072 1338-1346 NNP denotes Research
T4073 1347-1355 NNP denotes Products
T4074 1356-1357 -LRB- denotes (
T4075 1357-1363 NNP denotes Exeter
T4076 1363-1364 -COMMA- denotes ,
T4077 1365-1367 NNP denotes UK
T4078 1367-1368 -RRB- denotes )
T4079 1368-1369 -COMMA- denotes ,
T4080 1370-1373 CC denotes and
T4081 1374-1384 NN denotes antibodies
T4082 1385-1392 IN denotes against
T4083 1393-1399 NN denotes rabbit
T4084 1400-1414 JJ denotes IgG-conjugated
T4085 1415-1420 NN denotes TRITC
T4086 1421-1425 IN denotes from
T4087 1426-1430 NNP denotes Dako
T4088 1431-1441 NNP denotes Cytomation
T4089 1442-1443 -LRB- denotes (
T4090 1443-1452 NNP denotes Cambridge
T4091 1452-1453 -COMMA- denotes ,
T4092 1454-1456 NNP denotes UK
T4093 1456-1457 -RRB- denotes )
T4094 1459-1462 DT denotes The
T4095 1463-1470 JJ denotes anti-GR
T4096 1471-1479 NN denotes antibody
T4097 1480-1481 -LRB- denotes (
T4098 1481-1486 NN denotes Clone
T4099 1487-1489 CD denotes 41
T4100 1489-1490 -RRB- denotes )
T4101 1491-1495 IN denotes from
T4102 1496-1498 NN denotes BD
T4103 1499-1511 NN denotes Transduction
T4104 1512-1524 NN denotes Laboratories
T4105 1525-1526 -LRB- denotes (
T4106 1526-1532 NNP denotes Oxford
T4107 1532-1533 -COMMA- denotes ,
T4108 1534-1536 NNP denotes UK
T4109 1536-1537 -RRB- denotes )
T4110 1538-1541 VB denotes was
T4111 1542-1546 VB denotes used
T4112 1547-1550 IN denotes for
T4113 1551-1558 JJ denotes Western
T4114 1559-1563 NN denotes blot
T4115 1564-1572 NN denotes analysis
T4116 1574-1577 DT denotes All
T4117 1578-1583 JJ denotes other
T4118 1584-1592 NN denotes reagents
T4119 1593-1597 VB denotes were
T4120 1598-1607 VB denotes purchased
T4121 1608-1612 IN denotes from
T4122 1613-1618 NN denotes Sigma
T4123 1619-1620 -LRB- denotes (
T4124 1620-1625 NN denotes Poole
T4125 1625-1626 -COMMA- denotes ,
T4126 1627-1629 NNP denotes UK
T4127 1629-1630 -RRB- denotes )
T4354 1633-1637 NN denotes Cell
T4355 1638-1645 NN denotes Culture
T4356 1646-1649 CC denotes and
T4357 1650-1654 NN denotes PBMC
T4358 1655-1664 NN denotes Isolation
T4359 1665-1666 DT denotes A
T4360 1667-1672 JJ denotes human
T4361 1673-1674 NN denotes T
T4362 1675-1679 NN denotes cell
T4363 1680-1684 NN denotes line
T4364 1685-1686 -LRB- denotes (
T4365 1686-1692 NN denotes HuT-78
T4366 1692-1693 -RRB- denotes )
T4367 1694-1697 VB denotes was
T4368 1698-1707 VB denotes purchased
T4369 1708-1712 IN denotes from
T4370 1713-1718 NN denotes ECACC
T4371 1719-1727 NNP denotes European
T4372 1728-1738 NNP denotes Collection
T4373 1739-1741 IN denotes of
T4374 1742-1746 NNP denotes Cell
T4375 1747-1754 NNP denotes Culture
T4376 1755-1756 -LRB- denotes (
T4377 1756-1765 NNP denotes Wiltshire
T4378 1765-1766 -COMMA- denotes ,
T4379 1767-1769 NNP denotes UK
T4380 1769-1770 -RRB- denotes )
T4381 1771-1775 VB denotes were
T4382 1776-1784 VB denotes cultured
T4383 1785-1787 IN denotes as
T4384 1788-1798 RB denotes previously
T4385 1799-1808 VB denotes described
T4386 1809-1810 -LRB- denotes [
T4387 1810-1812 CD denotes 12
T4388 1812-1813 -RRB- denotes ]
T4389 1815-1820 NN denotes PBMCs
T4390 1821-1825 VB denotes were
T4391 1826-1834 VB denotes isolated
T4392 1835-1837 IN denotes by
T4393 1838-1845 NN denotes density
T4394 1846-1860 NN denotes centrifugation
T4395 1861-1865 IN denotes over
T4396 1866-1880 JJ denotes Ficoll-Hypaque
T4397 1881-1882 -LRB- denotes (
T4398 1882-1889 NN denotes density
T4399 1889-1890 -COMMA- denotes ,
T4400 1891-1896 CD denotes 1.077
T4401 1897-1901 NN denotes g/ml
T4402 1901-1902 -COLON- denotes ;
T4403 1903-1911 NNP denotes Amersham
T4404 1912-1923 NNP denotes Biosciences
T4405 1923-1924 -COMMA- denotes ,
T4406 1925-1933 NNP denotes Amersham
T4407 1933-1934 -COMMA- denotes ,
T4408 1935-1937 NNP denotes UK
T4409 1937-1938 -RRB- denotes )
T4410 1939-1941 IN denotes as
T4411 1942-1952 RB denotes previously
T4412 1953-1962 VB denotes described
T4413 1963-1964 -LRB- denotes [
T4414 1964-1966 CD denotes 34
T4415 1966-1967 -RRB- denotes ]
T4416 1969-1974 NN denotes Cells
T4417 1975-1979 VB denotes were
T4418 1980-1990 VB denotes stimulated
T4419 1991-1995 IN denotes with
T4420 1996-2009 JJ denotes anti-CD3/CD28
T4421 2010-2011 -LRB- denotes (
T4422 2011-2012 CD denotes 1
T4423 2013-2018 NN denotes µg/ml
T4424 2019-2023 DT denotes each
T4425 2023-2024 -RRB- denotes )
T4426 2025-2028 IN denotes for
T4427 2029-2030 CD denotes 1
T4428 2031-2032 NN denotes h
T4429 2033-2035 IN denotes at
T4430 2036-2040 NN denotes 37°C
T4431 2041-2043 TO denotes to
T4432 2044-2053 VB denotes stimulate
T4433 2054-2057 NN denotes Th2
T4434 2058-2066 NN denotes cytokine
T4435 2067-2074 NN denotes release
T4436 2075-2077 IN denotes in
T4437 2078-2081 DT denotes the
T4438 2082-2090 NN denotes presence
T4439 2091-2093 CC denotes or
T4440 2094-2101 NN denotes absence
T4441 2102-2104 IN denotes of
T4442 2105-2107 NN denotes FP
T4443 2108-2109 -LRB- denotes (
T4444 2109-2114 CD denotes 10−12
T4445 2115-2117 TO denotes to
T4446 2118-2123 NN denotes 10−8M
T4447 2123-2124 -RRB- denotes )
T4448 2126-2135 NN denotes Cytospins
T4449 2136-2140 VB denotes were
T4450 2141-2149 VB denotes prepared
T4451 2150-2153 CC denotes and
T4452 2154-2160 NN denotes GATA-3
T4453 2161-2173 NN denotes localization
T4454 2174-2184 VB denotes determined
T4455 2185-2187 IN denotes by
T4456 2188-2196 JJ denotes confocal
T4457 2197-2207 NN denotes microscopy
T4458 2208-2210 IN denotes as
T4459 2211-2221 RB denotes previously
T4460 2222-2231 VB denotes described
T4461 2232-2233 -LRB- denotes [
T4462 2233-2235 CD denotes 12
T4463 2235-2236 -RRB- denotes ]
T4622 2239-2246 JJ denotes Reverse
T4623 2247-2260 NN denotes Transcription
T4624 2261-2264 NN denotes PCR
T4625 2265-2270 JJ denotes Total
T4626 2271-2274 NN denotes RNA
T4627 2275-2278 VB denotes was
T4628 2279-2288 VB denotes extracted
T4629 2289-2294 VB denotes using
T4630 2295-2300 NN denotes lysis
T4631 2301-2307 NN denotes buffer
T4632 2308-2309 -LRB- denotes (
T4633 2309-2315 NN denotes RNeasy
T4634 2316-2319 NN denotes kit
T4635 2319-2320 -COLON- denotes ;
T4636 2321-2327 NNP denotes Qiagen
T4637 2327-2328 -COMMA- denotes ,
T4638 2329-2336 NNP denotes Crawley
T4639 2336-2337 -COMMA- denotes ,
T4640 2338-2340 NNP denotes UK
T4641 2340-2341 -RRB- denotes )
T4642 2343-2349 IN denotes During
T4643 2350-2353 NN denotes RNA
T4644 2354-2366 NN denotes purification
T4645 2366-2367 -COMMA- denotes ,
T4646 2368-2375 JJ denotes genomic
T4647 2376-2379 NN denotes DNA
T4648 2380-2383 VB denotes was
T4649 2384-2392 VB denotes digested
T4650 2393-2397 IN denotes with
T4651 2398-2408 JJ denotes RNase-free
T4652 2409-2414 NN denotes DNase
T4653 2415-2416 -LRB- denotes (
T4654 2416-2424 NNP denotes Amersham
T4655 2425-2436 NNP denotes Biosciences
T4656 2436-2437 -RRB- denotes )
T4657 2439-2443 RB denotes Next
T4658 2443-2444 -COMMA- denotes ,
T4659 2445-2448 CD denotes 0.5
T4660 2449-2451 NN denotes µg
T4661 2452-2454 IN denotes of
T4662 2455-2460 JJ denotes total
T4663 2461-2464 NN denotes RNA
T4664 2465-2468 VB denotes was
T4665 2469-2477 VB denotes reversed
T4666 2478-2489 VB denotes transcribed
T4667 2490-2495 VB denotes using
T4668 2496-2499 DT denotes the
T4669 2500-2505 JJ denotes avian
T4670 2506-2520 NN denotes myeloblastosis
T4671 2521-2526 NN denotes virus
T4672 2527-2529 NN denotes RT
T4673 2530-2531 -LRB- denotes (
T4674 2531-2538 NNP denotes Promega
T4675 2538-2539 -COMMA- denotes ,
T4676 2540-2551 NNP denotes Southampton
T4677 2551-2552 -COMMA- denotes ,
T4678 2553-2555 NNP denotes UK
T4679 2555-2556 -RRB- denotes )
T4680 2558-2561 IN denotes For
T4681 2562-2570 JJ denotes relative
T4682 2571-2585 NN denotes quantification
T4683 2585-2586 -COMMA- denotes ,
T4684 2587-2593 NN denotes RT-PCR
T4685 2594-2597 VB denotes was
T4686 2598-2605 VB denotes carried
T4687 2606-2609 RP denotes out
T4688 2610-2615 VB denotes using
T4689 2616-2620 NN denotes cDNA
T4690 2621-2627 NN denotes probes
T4691 2629-2636 NN denotes Primers
T4692 2637-2640 IN denotes for
T4693 2641-2645 NN denotes IL-4
T4694 2646-2650 VB denotes were
T4695 2651-2655 IN denotes from
T4696 2656-2669 NN denotes Sigma-Genosys
T4697 2670-2671 -LRB- denotes (
T4698 2671-2680 NNP denotes Cambridge
T4699 2680-2681 -COMMA- denotes ,
T4700 2682-2684 NNP denotes UK
T4701 2684-2685 -RRB- denotes )
T4702 2687-2696 NN denotes Sequences
T4703 2697-2699 IN denotes of
T4704 2700-2705 NN denotes GADPH
T4705 2706-2710 VB denotes used
T4706 2711-2714 VB denotes are
T4707 2715-2717 IN denotes as
T4708 2718-2725 VB denotes follows
T4709 2725-2726 -COLON- denotes :
T4710 2727-2734 RB denotes forward
T4711 2735-2761 NN denotes 5′-CCACCCATGGCAAATTCCATGGC
T4712 2761-2762 -COMMA- denotes ,
T4713 2763-2770 JJ denotes reverse
T4714 2771-2797 NN denotes 3′-TCTAGACGGCAGGTCAGGTCCAC
T4853 2800-2804 NNP denotes Cell
T4854 2805-2818 NNP denotes Fractionation
T4855 2818-2819 -COMMA- denotes ,
T4856 2820-2839 NN denotes Immunoprecipitation
T4857 2839-2840 -COMMA- denotes ,
T4858 2841-2844 CC denotes and
T4859 2845-2852 NNP denotes Western
T4860 2853-2857 NNP denotes Blot
T4861 2858-2866 NN denotes Analysis
T4862 2867-2874 JJ denotes Nuclear
T4863 2875-2878 CC denotes and
T4864 2879-2890 JJ denotes cytoplasmic
T4865 2891-2900 NN denotes fractions
T4866 2901-2905 VB denotes were
T4867 2906-2914 VB denotes prepared
T4868 2915-2917 IN denotes as
T4869 2918-2928 RB denotes previously
T4870 2929-2938 VB denotes described
T4871 2939-2940 -LRB- denotes [
T4872 2940-2942 CD denotes 35
T4873 2942-2943 -RRB- denotes ]
T4874 2945-2950 JJ denotes Whole
T4875 2951-2955 NN denotes cell
T4876 2956-2963 NN denotes lysates
T4877 2964-2968 VB denotes were
T4878 2969-2977 VB denotes prepared
T4879 2978-2980 IN denotes in
T4880 2981-2986 NN denotes NP-40
T4881 2987-2992 NN denotes lysis
T4882 2993-2999 NN denotes buffer
T4883 3000-3001 -LRB- denotes (
T4884 3001-3004 CD denotes 0.5
T4885 3004-3005 NN denotes %
T4886 3006-3013 NN denotes Nonidet
T4887 3014-3018 NN denotes P-40
T4888 3018-3019 -COMMA- denotes ,
T4889 3020-3022 CD denotes 20
T4890 3023-3025 NN denotes mM
T4891 3026-3034 NN denotes Tris-HCl
T4892 3035-3036 -LRB- denotes [
T4893 3036-3038 NN denotes pH
T4894 3039-3042 CD denotes 7.5
T4895 3042-3043 -RRB- denotes ]
T4896 3043-3044 -COMMA- denotes ,
T4897 3045-3048 CD denotes 150
T4898 3049-3051 NN denotes mM
T4899 3052-3056 NN denotes NaCl
T4900 3056-3057 -RRB- denotes )
T4901 3058-3060 IN denotes in
T4902 3061-3064 DT denotes the
T4903 3065-3073 NN denotes presence
T4904 3074-3076 IN denotes of
T4905 3077-3085 JJ denotes complete
T4906 3086-3094 NN denotes protease
T4907 3095-3103 NN denotes cocktail
T4908 3104-3113 NN denotes inhibitor
T4909 3115-3122 NN denotes Lysates
T4910 3123-3127 VB denotes were
T4911 3128-3139 VB denotes centrifuged
T4912 3140-3142 IN denotes at
T4913 3143-3146 NN denotes 4°C
T4914 3147-3150 IN denotes for
T4915 3151-3153 CD denotes 10
T4916 3154-3157 NN denotes min
T4917 3158-3160 IN denotes at
T4918 3161-3167 CD denotes 12,000
T4919 3168-3171 NN denotes rpm
T4920 3172-3174 IN denotes in
T4921 3175-3177 DT denotes an
T4922 3178-3187 NNP denotes Eppendorf
T4923 3188-3203 NN denotes microcentrifuge
T4924 3204-3206 TO denotes to
T4925 3207-3213 VB denotes remove
T4926 3214-3222 JJ denotes cellular
T4927 3223-3229 NN denotes debris
T4928 3231-3238 NN denotes Samples
T4929 3239-3243 VB denotes were
T4930 3244-3248 RB denotes then
T4931 3249-3267 VB denotes immunoprecipitated
T4932 3268-3272 IN denotes with
T4933 3273-3279 CC denotes either
T4934 3280-3282 CD denotes 10
T4935 3283-3285 NN denotes µl
T4936 3286-3288 IN denotes of
T4937 3289-3297 NN denotes antibody
T4938 3298-3305 IN denotes against
T4939 3306-3312 NN denotes GATA-3
T4940 3313-3315 CC denotes or
T4941 3316-3326 NN denotes importin-α
T4942 3327-3332 VB denotes using
T4943 3333-3336 NN denotes A/G
T4944 3337-3344 VB denotes agarose
T4945 3345-3351 NN denotes slurry
T4946 3352-3354 IN denotes in
T4947 3355-3358 DT denotes the
T4948 3359-3367 NN denotes presence
T4949 3368-3370 IN denotes of
T4950 3371-3379 NN denotes protease
T4951 3380-3389 NN denotes inhibitor
T4952 3390-3395 VB denotes using
T4953 3396-3399 DT denotes the
T4954 3400-3405 NNP denotes Catch
T4955 3406-3409 CC denotes and
T4956 3410-3417 NN denotes Release
T4957 3418-3429 NN denotes methodology
T4958 3430-3431 -LRB- denotes (
T4959 3431-3438 NNP denotes Upstate
T4960 3439-3452 NNP denotes Biotechnology
T4961 3452-3453 -COMMA- denotes ,
T4962 3454-3458 NNP denotes Lake
T4963 3459-3465 NNP denotes Placid
T4964 3465-3466 -COMMA- denotes ,
T4965 3467-3470 NNP denotes New
T4966 3471-3475 NNP denotes York
T4967 3475-3476 -COMMA- denotes ,
T4968 3477-3480 NNP denotes USA
T4969 3480-3481 -RRB- denotes )
T4970 3483-3490 NN denotes Western
T4971 3491-3495 NN denotes blot
T4972 3496-3504 NN denotes analysis
T4973 3505-3508 VB denotes was
T4974 3509-3518 VB denotes performed
T4975 3519-3524 VB denotes using
T4976 3525-3536 NN denotes anti-GATA-3
T4977 3536-3537 -COMMA- denotes ,
T4978 3538-3553 JJ denotes anti-importin-α
T4979 3553-3554 -COMMA- denotes ,
T4980 3555-3562 JJ denotes anti-GR
T4981 3562-3563 -COMMA- denotes ,
T4982 3564-3574 JJ denotes anti-p-p38
T4983 3575-3578 NN denotes MAP
T4984 3579-3585 NN denotes kinase
T4985 3585-3586 -COMMA- denotes ,
T4986 3587-3599 NN denotes anti-p-ATF-2
T4987 3599-3600 -COMMA- denotes ,
T4988 3601-3604 CC denotes and
T4989 3605-3618 JJ denotes anti-p-serine
T4990 3620-3634 JJ denotes Immunoreactive
T4991 3635-3643 NN denotes proteins
T4992 3644-3648 VB denotes were
T4993 3649-3657 VB denotes detected
T4994 3658-3663 VB denotes using
T4995 3664-3666 DT denotes an
T4996 3667-3675 VB denotes enhanced
T4997 3676-3693 NN denotes chemiluminescence
T4998 3694-3697 NN denotes ECL
T4999 3698-3701 NN denotes kit
T5000 3702-3703 -LRB- denotes (
T5001 3703-3711 NNP denotes Amersham
T5002 3712-3723 NNP denotes Biosciences
T5003 3723-3724 -RRB- denotes )
T5206 3727-3736 NN denotes Chromatin
T5207 3737-3756 NN denotes Immunoprecipitation
T5208 3757-3766 NN denotes Chromatin
T5209 3767-3786 NN denotes immunoprecipitation
T5210 3787-3788 -LRB- denotes (
T5211 3788-3790 NN denotes IP
T5212 3790-3791 -RRB- denotes )
T5213 3792-3795 VB denotes was
T5214 3796-3805 VB denotes performed
T5215 3806-3808 IN denotes as
T5216 3809-3819 RB denotes previously
T5217 3820-3829 VB denotes described
T5218 3830-3831 -LRB- denotes [
T5219 3831-3833 CD denotes 12
T5220 3833-3834 -RRB- denotes ]
T5221 3835-3837 IN denotes in
T5222 3838-3844 JJ denotes HuT-78
T5223 3845-3852 NN denotes T-cells
T5224 3853-3857 IN denotes with
T5225 3858-3859 CD denotes 2
T5226 3860-3862 NN denotes µg
T5227 3863-3865 IN denotes of
T5228 3866-3877 NN denotes anti-GATA-3
T5229 3878-3879 -LRB- denotes (
T5230 3879-3884 NNP denotes Santa
T5231 3885-3889 NNP denotes Cruz
T5232 3890-3903 NNP denotes Biotechnology
T5233 3903-3904 -RRB- denotes )
T5234 3904-3905 -COMMA- denotes ,
T5235 3906-3908 CC denotes or
T5236 3909-3917 JJ denotes isotypic
T5237 3918-3932 NN denotes immunoglobulin
T5238 3933-3934 NN denotes G
T5239 3935-3937 IN denotes as
T5240 3938-3939 DT denotes a
T5241 3940-3952 JJ denotes non-specific
T5242 3953-3960 NN denotes control
T5243 3961-3962 -LRB- denotes (
T5244 3962-3967 NNP denotes Santa
T5245 3968-3972 NNP denotes Cruz
T5246 3973-3986 NNP denotes Biotechnology
T5247 3986-3987 -RRB- denotes )
T5248 3988-3997 RB denotes overnight
T5249 3998-4000 IN denotes at
T5250 4001-4005 NNP denotes 4°C.
T5251 4006-4014 NNP denotes Promoter
T5252 4015-4024 NN denotes sequences
T5253 4025-4029 VB denotes were
T5254 4030-4038 VB denotes detected
T5255 4039-4043 IN denotes with
T5256 4044-4047 NN denotes PCR
T5257 4048-4055 NN denotes primers
T5258 4056-4059 IN denotes for
T5259 4060-4063 DT denotes the
T5260 4064-4068 NN denotes IL-5
T5261 4069-4077 NN denotes promoter
T5262 4078-4079 -LRB- denotes (
T5263 4079-4083 CD denotes −445
T5264 4084-4086 TO denotes to
T5265 4087-4089 CD denotes +4
T5266 4089-4090 -RRB- denotes )
T5267 4090-4091 -COLON- denotes :
T5268 4092-4099 JJ denotes forward
T5269 4100-4127 NN denotes 5′-TTAATCTAGCCACAGTCATAG-3′
T5270 4128-4131 CC denotes and
T5271 4132-4139 VB denotes reverse
T5272 4139-4140 -COLON- denotes :
T5273 4141-4168 NN denotes 5′-TCATGGCTCTGAAACGTTCTG-3′
T5274 4170-4173 NN denotes PCR
T5275 4174-4177 VB denotes was
T5276 4178-4187 VB denotes performed
T5277 4188-4193 VB denotes using
T5278 4194-4195 DT denotes a
T5279 4196-4202 NNP denotes Hybaid
T5280 4203-4211 NNP denotes Omnigene
T5281 4212-4219 JJ denotes thermal
T5282 4220-4226 NN denotes cycler
T5283 4227-4228 -LRB- denotes (
T5284 4228-4234 NNP denotes Hybaid
T5285 4234-4235 -COMMA- denotes ,
T5286 4236-4243 NNP denotes Ashford
T5287 4243-4244 -COMMA- denotes ,
T5288 4245-4247 NNP denotes UK
T5289 4247-4248 -RRB- denotes )
T5290 4249-4253 IN denotes with
T5291 4254-4261 VB denotes cycling
T5292 4262-4272 NN denotes parameters
T5293 4273-4275 IN denotes of
T5294 4276-4280 NN denotes 72°C
T5295 4281-4284 IN denotes for
T5296 4285-4287 CD denotes 10
T5297 4288-4291 NN denotes min
T5298 4291-4292 -COMMA- denotes ,
T5301 4303-4305 IN denotes at
T5302 4306-4310 NN denotes 94°C
T5303 4311-4314 IN denotes for
T5304 4315-4317 CD denotes 45
T5305 4318-4319 NN denotes s
T5306 4319-4320 -COMMA- denotes ,
T5307 4321-4325 NN denotes 52°C
T5308 4326-4329 IN denotes for
T5309 4330-4332 CD denotes 45
T5310 4333-4334 NN denotes s
T5311 4334-4335 -COMMA- denotes ,
T5312 4336-4340 NN denotes 72°C
T5313 4341-4344 IN denotes for
T5314 4345-4347 CD denotes 45
T5315 4348-4349 NN denotes s
T5499 4352-4361 NN denotes GR-GATA-3
T5500 4362-4364 IN denotes In
T5501 4365-4370 NNP denotes Vitro
T5502 4371-4382 NNP denotes Competition
T5503 4383-4388 NNP denotes Assay
T5504 4389-4392 IN denotes for
T5505 4393-4403 NNP denotes Importin-α
T5506 4404-4405 -LRB- denotes (
T5507 4405-4416 JJ denotes Far-Western
T5508 4417-4422 NN denotes ELISA
T5509 4422-4423 -RRB- denotes )
T5510 4424-4442 VB denotes Immunoprecipitated
T5511 4443-4453 JJ denotes importin-α
T5512 4454-4455 -LRB- denotes (
T5513 4455-4470 JJ denotes anti-importin-α
T5514 4470-4471 -COMMA- denotes ,
T5515 4472-4477 NNP denotes Santa
T5516 4478-4482 NNP denotes Cruz
T5517 4483-4496 NNP denotes Biotechnology
T5518 4496-4497 -RRB- denotes )
T5519 4498-4502 IN denotes from
T5520 4503-4509 NN denotes HuT-78
T5521 4510-4515 NN denotes cells
T5522 4516-4519 VB denotes was
T5523 4520-4529 VB denotes separated
T5524 4530-4532 IN denotes by
T5525 4533-4541 NN denotes SDS-PAGE
T5526 4542-4545 CC denotes and
T5527 4546-4554 VB denotes purified
T5528 4555-4559 IN denotes from
T5529 4560-4563 DT denotes the
T5530 4564-4571 VB denotes excised
T5531 4572-4575 NN denotes gel
T5532 4576-4578 IN denotes by
T5533 4579-4593 NN denotes electroelution
T5534 4594-4595 -LRB- denotes [
T5535 4595-4597 CD denotes 36
T5536 4597-4598 -RRB- denotes ]
T5537 4600-4609 RB denotes Similarly
T5538 4609-4610 -COMMA- denotes ,
T5539 4611-4617 NN denotes GATA-3
T5540 4618-4621 VB denotes was
T5541 4622-4630 VB denotes isolated
T5542 4631-4635 IN denotes from
T5543 4636-4649 JJ denotes nonstimulated
T5544 4650-4656 NN denotes HuT-78
T5545 4657-4662 NN denotes cells
T5546 4663-4665 CC denotes or
T5547 4666-4671 NN denotes cells
T5548 4672-4682 VB denotes stimulated
T5549 4683-4686 IN denotes for
T5550 4687-4689 CD denotes 30
T5551 4690-4693 NN denotes min
T5552 4694-4698 IN denotes with
T5553 4699-4712 JJ denotes anti-CD3/CD28
T5554 4713-4716 CC denotes and
T5555 4717-4719 NN denotes GR
T5556 4720-4723 VB denotes was
T5557 4724-4732 VB denotes isolated
T5558 4733-4737 IN denotes from
T5559 4738-4740 NN denotes FP
T5560 4741-4742 -LRB- denotes (
T5561 4742-4746 CD denotes 10−8
T5562 4747-4748 NN denotes M
T5563 4748-4749 -COMMA- denotes ,
T5564 4750-4752 CD denotes 30
T5565 4753-4756 NN denotes min
T5566 4756-4757 -RRB- denotes )
T5567 4758-4768 VB denotes stimulated
T5568 4769-4775 NN denotes HuT-78
T5569 4776-4781 NN denotes cells
T5570 4783-4788 DT denotes These
T5571 4789-4797 NN denotes proteins
T5572 4798-4802 VB denotes were
T5573 4803-4815 RB denotes subsequently
T5574 4816-4824 VB denotes refolded
T5575 4825-4827 IN denotes in
T5576 4828-4835 NN denotes glycine
T5577 4836-4844 NN denotes solution
T5578 4846-4849 CD denotes 100
T5579 4850-4852 NN denotes µl
T5580 4853-4855 IN denotes of
T5581 4856-4866 JJ denotes importin-α
T5582 4867-4875 NN denotes solution
T5583 4876-4877 -LRB- denotes (
T5584 4877-4880 CD denotes 100
T5585 4881-4886 NN denotes ng/ml
T5586 4887-4889 IN denotes in
T5587 4890-4893 NN denotes TBS
T5588 4893-4894 -RRB- denotes )
T5589 4895-4898 VB denotes was
T5590 4899-4904 VB denotes added
T5591 4905-4907 TO denotes to
T5592 4908-4915 JJ denotes 96-well
T5593 4916-4922 NN denotes plates
T5594 4923-4929 VB denotes coated
T5595 4930-4934 IN denotes with
T5596 4935-4939 NN denotes goat
T5597 4940-4955 JJ denotes anti-importin-α
T5598 4956-4964 NN denotes antibody
T5599 4966-4971 IN denotes After
T5600 4972-4973 CD denotes 1
T5601 4974-4975 NN denotes h
T5602 4976-4986 NN denotes incubation
T5603 4986-4987 -COMMA- denotes ,
T5604 4988-4994 NN denotes GATA-3
T5605 4995-4996 -LRB- denotes (
T5606 4996-4999 CD denotes 100
T5607 5000-5005 NN denotes ng/ml
T5608 5006-5008 IN denotes in
T5609 5009-5012 NN denotes TBS
T5610 5012-5013 -RRB- denotes )
T5611 5014-5018 IN denotes from
T5612 5019-5029 VB denotes stimulated
T5613 5030-5032 CC denotes or
T5614 5033-5045 JJ denotes unstimulated
T5615 5046-5051 NN denotes cells
T5616 5052-5056 VB denotes were
T5617 5057-5062 VB denotes added
T5618 5063-5065 TO denotes to
T5619 5066-5069 DT denotes the
T5620 5070-5087 JJ denotes importin-α-coated
T5621 5088-5093 NN denotes wells
T5622 5095-5097 NN denotes GR
T5623 5098-5099 -LRB- denotes (
T5624 5099-5101 CD denotes 10
T5625 5102-5104 CC denotes or
T5626 5105-5108 CD denotes 100
T5627 5109-5114 NN denotes ng/ml
T5628 5115-5117 IN denotes in
T5629 5118-5121 NN denotes TBS
T5630 5121-5122 -RRB- denotes )
T5631 5123-5127 IN denotes from
T5632 5128-5138 VB denotes stimulated
T5633 5139-5141 CC denotes or
T5634 5142-5154 JJ denotes unstimulated
T5635 5155-5160 NN denotes cells
T5636 5161-5165 VB denotes were
T5637 5166-5171 VB denotes added
T5638 5172-5174 TO denotes to
T5639 5175-5179 DT denotes some
T5640 5180-5185 NN denotes wells
T5641 5187-5192 IN denotes After
T5642 5193-5194 DT denotes a
T5643 5195-5202 JJ denotes further
T5644 5203-5204 CD denotes 1
T5645 5205-5206 NN denotes h
T5646 5207-5217 NN denotes incubation
T5647 5217-5218 -COMMA- denotes ,
T5648 5219-5222 DT denotes the
T5649 5223-5228 NN denotes plate
T5650 5229-5232 VB denotes was
T5651 5233-5239 VB denotes washed
T5652 5240-5243 CC denotes and
T5653 5244-5253 VB denotes incubated
T5654 5254-5258 IN denotes with
T5655 5259-5266 JJ denotes primary
T5656 5267-5277 NN denotes antibodies
T5657 5278-5279 -LRB- denotes (
T5658 5279-5280 DT denotes a
T5659 5281-5288 NN denotes mixture
T5660 5289-5291 IN denotes of
T5661 5292-5298 NN denotes rabbit
T5662 5299-5310 NN denotes anti-GATA-3
T5663 5311-5314 CC denotes and
T5664 5315-5320 NN denotes mouse
T5665 5321-5328 NN denotes anti-GR
T5666 5328-5329 -COMMA- denotes ,
T5667 5330-5335 NNP denotes Santa
T5668 5336-5340 NNP denotes Cruz
T5669 5341-5354 NNP denotes Biotechnology
T5670 5354-5355 -RRB- denotes )
T5671 5356-5359 IN denotes for
T5672 5360-5363 CD denotes 1.5
T5673 5364-5365 NN denotes h
T5674 5365-5366 -COMMA- denotes ,
T5675 5367-5370 CC denotes and
T5676 5371-5375 RB denotes then
T5677 5376-5385 VB denotes incubated
T5678 5386-5390 IN denotes with
T5679 5391-5400 JJ denotes secondary
T5680 5401-5411 NN denotes antibodies
T5681 5413-5417 NN denotes FITC
T5682 5418-5423 NN denotes swine
T5683 5424-5435 JJ denotes anti-rabbit
T5684 5436-5439 NN denotes IgG
T5685 5440-5441 -LRB- denotes (
T5686 5441-5445 NNP denotes Dako
T5687 5445-5446 -COMMA- denotes ,
T5688 5447-5456 NNP denotes Cambridge
T5689 5456-5457 -COMMA- denotes ,
T5690 5458-5460 NNP denotes UK
T5691 5460-5461 -RRB- denotes )
T5692 5462-5465 VB denotes was
T5693 5466-5470 VB denotes used
T5694 5471-5474 IN denotes for
T5695 5475-5478 DT denotes the
T5696 5479-5488 NN denotes detection
T5697 5489-5491 IN denotes of
T5698 5492-5498 NN denotes GATA-3
T5699 5498-5499 -COMMA- denotes ,
T5700 5500-5503 CC denotes and
T5701 5504-5513 NN denotes rhodamine
T5702 5514-5520 NN denotes donkey
T5703 5521-5531 JJ denotes anti-mouse
T5704 5532-5535 NN denotes IgG
T5705 5536-5537 -LRB- denotes (
T5706 5537-5542 NNP denotes Novus
T5707 5542-5543 -COMMA- denotes ,
T5708 5544-5553 NNP denotes Littleton
T5709 5553-5554 -COMMA- denotes ,
T5710 5555-5563 NNP denotes Colorado
T5711 5563-5564 -COMMA- denotes ,
T5712 5565-5568 NNP denotes USA
T5713 5568-5569 -RRB- denotes )
T5714 5570-5573 VB denotes was
T5715 5574-5578 VB denotes used
T5716 5579-5582 IN denotes for
T5717 5583-5586 DT denotes the
T5718 5587-5596 NN denotes detection
T5719 5597-5599 IN denotes of
T5720 5600-5602 NN denotes GR
T5721 5604-5608 NN denotes FITC
T5722 5609-5612 CC denotes and
T5723 5613-5622 NN denotes rhodamine
T5724 5623-5629 NN denotes levels
T5725 5630-5634 VB denotes were
T5726 5635-5643 VB denotes measured
T5727 5644-5648 IN denotes with
T5728 5649-5650 DT denotes a
T5729 5651-5662 JJ denotes fluorescent
T5730 5663-5674 NN denotes micro-plate
T5731 5675-5681 NN denotes reader
T5732 5682-5683 -LRB- denotes (
T5733 5683-5690 NNP denotes Bioline
T5734 5690-5691 -COMMA- denotes ,
T5735 5692-5698 NNP denotes London
T5736 5698-5699 -COMMA- denotes ,
T5737 5700-5702 NNP denotes UK
T5738 5702-5703 -RRB- denotes )
T6080 5706-5711 NN denotes NF-κB
T6081 5712-5722 NN denotes Activation
T6082 5723-5728 NN denotes NF-κB
T6083 5729-5736 NN denotes binding
T6084 5737-5745 NN denotes activity
T6085 5746-5748 IN denotes in
T6086 5749-5756 JJ denotes nuclear
T6087 5757-5765 NN denotes extracts
T6088 5766-5769 VB denotes was
T6089 5770-5780 VB denotes determined
T6090 5781-5786 VB denotes using
T6091 5787-5789 DT denotes an
T6092 5790-5801 JJ denotes ELISA-based
T6093 5802-5805 NN denotes kit
T6094 5806-5807 -LRB- denotes (
T6095 5807-5815 NN denotes Trans-AM
T6096 5816-5819 NN denotes p65
T6097 5819-5820 -COMMA- denotes ,
T6098 5821-5827 NNP denotes Active
T6099 5828-5833 NNP denotes Motif
T6100 5833-5834 -COMMA- denotes ,
T6101 5835-5844 NNP denotes Rixensart
T6102 5844-5845 -COMMA- denotes ,
T6103 5846-5853 NNP denotes Belgium
T6104 5853-5854 -RRB- denotes )
T6105 5856-5858 IN denotes In
T6106 5859-5864 NN denotes brief
T6107 5864-5865 -COMMA- denotes ,
T6108 5866-5867 CD denotes 5
T6109 5868-5870 NN denotes µg
T6110 5871-5873 IN denotes of
T6111 5874-5881 JJ denotes nuclear
T6112 5882-5890 NN denotes extracts
T6113 5891-5895 VB denotes were
T6114 5896-5905 VB denotes incubated
T6115 5906-5910 IN denotes with
T6116 5911-5912 DT denotes a
T6117 5913-5918 NN denotes plate
T6118 5919-5925 VB denotes coated
T6119 5926-5930 IN denotes with
T6120 5931-5933 DT denotes an
T6121 5934-5939 NN denotes NF-κB
T6122 5940-5949 NN denotes consensus
T6123 5950-5965 NN denotes oligonucleotide
T6124 5967-5973 NN denotes Plates
T6125 5974-5978 VB denotes were
T6126 5979-5985 VB denotes washed
T6127 5986-5992 IN denotes before
T6128 5993-6001 NN denotes addition
T6129 6002-6004 IN denotes of
T6130 6005-6007 DT denotes an
T6131 6008-6016 JJ denotes anti-p65
T6132 6017-6025 NN denotes antibody
T6133 6027-6035 NN denotes Antibody
T6134 6036-6043 NN denotes binding
T6135 6044-6047 VB denotes was
T6136 6048-6056 VB denotes detected
T6137 6057-6061 IN denotes with
T6138 6062-6063 DT denotes a
T6139 6064-6073 JJ denotes secondary
T6140 6074-6088 JJ denotes HRP-conjugated
T6141 6089-6097 NN denotes antibody
T6142 6098-6101 CC denotes and
T6143 6102-6111 VB denotes developed
T6144 6112-6116 IN denotes with
T6145 6117-6120 NN denotes TMB
T6146 6121-6130 NN denotes substrate
T6147 6132-6135 DT denotes The
T6148 6136-6145 NN denotes intensity
T6149 6146-6148 IN denotes of
T6150 6149-6152 DT denotes the
T6151 6153-6161 NN denotes reaction
T6152 6162-6165 VB denotes was
T6153 6166-6174 VB denotes measured
T6154 6175-6177 IN denotes at
T6155 6178-6181 CD denotes 450
T6156 6182-6184 NN denotes nm
T6283 6187-6205 NN denotes Immunofluorescence
T6284 6206-6214 NN denotes Staining
T6285 6215-6233 NN denotes Immunofluorescence
T6286 6234-6242 NN denotes staining
T6287 6243-6246 VB denotes was
T6288 6247-6256 VB denotes performed
T6289 6257-6259 IN denotes as
T6290 6260-6270 RB denotes previously
T6291 6271-6280 VB denotes described
T6292 6281-6282 -LRB- denotes [
T6293 6282-6284 CD denotes 34
T6294 6284-6285 -RRB- denotes ]
T6295 6287-6290 DT denotes All
T6296 6291-6299 NN denotes staining
T6297 6300-6303 VB denotes was
T6298 6304-6313 VB denotes performed
T6299 6314-6316 IN denotes at
T6300 6317-6321 NN denotes room
T6301 6322-6333 NN denotes temperature
T6302 6334-6337 CC denotes and
T6303 6338-6343 IN denotes under
T6304 6344-6358 NN denotes humidification
T6305 6360-6365 NN denotes Cells
T6306 6366-6370 VB denotes were
T6307 6371-6380 VB denotes collected
T6308 6381-6384 CC denotes and
T6309 6385-6394 NN denotes cytospins
T6310 6395-6403 VB denotes prepared
T6311 6404-6406 IN denotes in
T6312 6407-6408 DT denotes a
T6313 6409-6423 NN denotes cytocentrifuge
T6314 6424-6425 -LRB- denotes (
T6315 6425-6432 NNP denotes Shandon
T6316 6433-6435 NNP denotes II
T6317 6435-6436 -COMMA- denotes ,
T6318 6437-6444 NNP denotes Shandon
T6319 6444-6445 -COMMA- denotes ,
T6320 6446-6453 NNP denotes Runcorn
T6321 6453-6454 -COMMA- denotes ,
T6322 6455-6457 NNP denotes UK
T6323 6457-6458 -RRB- denotes )
T6324 6460-6465 NN denotes Cells
T6325 6466-6470 VB denotes were
T6326 6471-6484 VB denotes permeabilized
T6327 6484-6485 -COMMA- denotes ,
T6328 6486-6493 VB denotes blocked
T6329 6493-6494 -COMMA- denotes ,
T6330 6495-6498 CC denotes and
T6331 6499-6503 RB denotes then
T6332 6504-6513 VB denotes incubated
T6333 6514-6518 IN denotes with
T6334 6519-6521 NN denotes GR
T6335 6522-6523 -LRB- denotes (
T6336 6523-6527 CD denotes 1∶50
T6337 6528-6532 NN denotes E-20
T6338 6533-6538 NNP denotes Santa
T6339 6539-6543 NNP denotes Cruz
T6340 6544-6557 NNP denotes Biotechnology
T6341 6557-6558 -RRB- denotes )
T6342 6559-6561 CC denotes or
T6343 6562-6573 NN denotes anti-GATA-3
T6344 6574-6575 -LRB- denotes (
T6345 6575-6579 NN denotes H-48
T6346 6579-6580 -COLON- denotes ;
T6347 6581-6586 NNP denotes Santa
T6348 6587-6591 NNP denotes Cruz
T6349 6592-6605 NNP denotes Biotechnology
T6350 6605-6606 -RRB- denotes )
T6351 6607-6615 NN denotes antibody
T6352 6616-6619 IN denotes for
T6353 6620-6621 CD denotes 1
T6354 6622-6623 NN denotes h
T6355 6624-6626 IN denotes at
T6356 6627-6631 NN denotes room
T6357 6632-6643 NN denotes temperature
T6358 6645-6650 IN denotes After
T6359 6651-6656 CD denotes three
T6360 6657-6663 NN denotes washes
T6361 6664-6666 IN denotes in
T6362 6667-6685 JJ denotes phosphate-buffered
T6363 6686-6692 NN denotes saline
T6364 6693-6694 -LRB- denotes (
T6365 6694-6697 NN denotes PBS
T6366 6697-6698 -RRB- denotes )
T6367 6698-6699 -COMMA- denotes ,
T6368 6700-6705 NN denotes cells
T6369 6706-6710 VB denotes were
T6370 6711-6720 VB denotes incubated
T6371 6721-6725 IN denotes with
T6372 6726-6740 NN denotes tetrarhodamine
T6373 6741-6766 VB denotes isothiocyanate–conjugated
T6374 6767-6771 NN denotes goat
T6375 6772-6783 JJ denotes anti-rabbit
T6376 6784-6792 NN denotes antibody
T6377 6793-6794 -LRB- denotes (
T6378 6794-6798 NN denotes Dako
T6379 6798-6799 -RRB- denotes )
T6380 6801-6810 NN denotes Cytospins
T6381 6811-6815 VB denotes were
T6382 6816-6830 VB denotes counterstained
T6383 6831-6835 IN denotes with
T6384 6836-6838 NN denotes 4′
T6385 6838-6839 -COMMA- denotes ,
T6386 6839-6865 NN denotes 6-diamidino-2-phenylindole
T6387 6866-6881 NN denotes dihydrochloride
T6388 6882-6883 -LRB- denotes (
T6389 6883-6887 NN denotes DAPI
T6390 6887-6888 -RRB- denotes )
T6391 6888-6889 -COMMA- denotes ,
T6392 6890-6891 DT denotes a
T6393 6892-6903 JJ denotes fluorescent
T6394 6904-6908 JJ denotes blue
T6395 6909-6916 JJ denotes nuclear
T6396 6917-6923 NN denotes indole
T6397 6924-6933 NN denotes chromatin
T6398 6934-6939 NN denotes stain
T6399 6939-6940 -COMMA- denotes ,
T6400 6941-6944 CC denotes and
T6401 6945-6952 VB denotes mounted
T6402 6953-6955 IN denotes in
T6403 6956-6959 NNP denotes PBS
T6404 6959-6960 -COLON- denotes :
T6405 6960-6968 NN denotes glycerol
T6406 6969-6970 -LRB- denotes (
T6407 6970-6975 CD denotes 50∶50
T6408 6975-6976 -RRB- denotes )
T6409 6978-6981 DT denotes The
T6410 6982-6996 JJ denotes immunopositive
T6411 6997-7003 NN denotes signal
T6412 7004-7007 VB denotes was
T6413 7008-7021 VB denotes characterised
T6414 7022-7027 VB denotes using
T6415 7028-7033 NN denotes laser
T6416 7034-7042 NN denotes scanning
T6417 7043-7051 JJ denotes confocal
T6418 7052-7062 NN denotes microscopy
T6419 7063-7065 IN denotes on
T6420 7066-7067 DT denotes a
T6421 7068-7073 NNP denotes Leica
T6422 7074-7077 NNP denotes TCS
T6423 7078-7083 NNP denotes NT/SP
T6424 7084-7095 JJ denotes interactive
T6425 7096-7101 NN denotes laser
T6426 7102-7111 NN denotes cytometer
T6427 7112-7120 VB denotes equipped
T6428 7121-7125 IN denotes with
T6429 7126-7134 JJ denotes confocal
T6430 7135-7141 NN denotes optics
T6431 7142-7143 -LRB- denotes (
T6432 7143-7148 NNP denotes Leica
T6433 7149-7161 NNP denotes Microsystems
T6434 7161-7162 -COMMA- denotes ,
T6435 7163-7170 NNP denotes Wetzlar
T6436 7170-7171 -COMMA- denotes ,
T6437 7172-7179 NNP denotes Germany
T6438 7179-7180 -RRB- denotes )
T6439 7182-7184 TO denotes To
T6440 7185-7194 VB denotes determine
T6441 7195-7198 DT denotes the
T6442 7199-7210 NN denotes specificity
T6443 7211-7213 IN denotes of
T6444 7214-7217 DT denotes the
T6445 7218-7228 NN denotes antibodies
T6446 7228-7229 -COMMA- denotes ,
T6447 7230-7236 NN denotes rabbit
T6448 7237-7242 NN denotes serum
T6449 7243-7257 NN denotes immunoglobulin
T6450 7258-7259 -LRB- denotes (
T6451 7259-7263 NN denotes Dako
T6452 7263-7264 -RRB- denotes )
T6453 7265-7268 CC denotes and
T6454 7269-7278 JJ denotes secondary
T6455 7279-7289 NN denotes antibodies
T6456 7290-7297 IN denotes without
T6457 7298-7301 DT denotes the
T6458 7302-7309 JJ denotes primary
T6459 7310-7314 VB denotes were
T6460 7315-7319 VB denotes used
T6461 7320-7322 IN denotes as
T6462 7323-7331 NN denotes controls
T6463 7333-7343 RB denotes Positively
T6464 7344-7351 VB denotes stained
T6465 7352-7358 NN denotes nuclei
T6466 7359-7362 CC denotes and
T6467 7363-7368 JJ denotes total
T6468 7369-7374 NN denotes cells
T6469 7375-7379 VB denotes were
T6470 7380-7387 VB denotes counted
T6471 7388-7389 -LRB- denotes (
T6472 7389-7392 CD denotes 500
T6473 7392-7393 -RRB- denotes )
T6474 7394-7396 IN denotes on
T6475 7397-7401 DT denotes each
T6476 7402-7407 NN denotes slide
T6477 7408-7412 IN denotes with
T6478 7413-7416 DT denotes the
T6479 7417-7425 NN denotes observer
T6480 7426-7433 VB denotes blinded
T6481 7434-7436 TO denotes to
T6482 7437-7440 DT denotes the
T6483 7441-7450 NN denotes treatment
T6779 7453-7463 NN denotes GATA-3-GFP
T6780 7464-7473 NN denotes Construct
T6781 7474-7477 DT denotes The
T6782 7478-7484 NN denotes GATA-3
T6783 7485-7490 NN denotes clone
T6784 7491-7492 -LRB- denotes (
T6785 7492-7500 NN denotes BC003070
T6786 7500-7501 -RRB- denotes )
T6787 7502-7510 JJ denotes complete
T6788 7511-7515 NN denotes cDNA
T6789 7516-7519 VB denotes was
T6790 7520-7528 VB denotes obtained
T6791 7529-7533 IN denotes from
T6792 7534-7544 NNP denotes Invitrogen
T6793 7545-7549 NNP denotes Life
T6794 7550-7562 NNP denotes Technologies
T6795 7563-7565 IN denotes as
T6796 7566-7567 DT denotes a
T6797 7568-7571 JJ denotes 5′-
T6798 7572-7585 NN denotes EcoRI/3′-XhoI
T6799 7586-7592 NN denotes insert
T6800 7593-7595 IN denotes of
T6801 7596-7602 NN denotes GATA-3
T6802 7603-7605 IN denotes in
T6803 7606-7609 DT denotes the
T6804 7610-7615 NN denotes pOTB7
T6805 7616-7622 NN denotes vector
T6806 7624-7630 NN denotes GATA-3
T6807 7631-7634 VB denotes was
T6808 7635-7642 VB denotes excised
T6809 7643-7647 IN denotes from
T6810 7648-7653 NN denotes pOTB7
T6811 7654-7659 VB denotes using
T6812 7660-7664 NN denotes XhoI
T6813 7665-7674 NN denotes digestion
T6814 7675-7678 CC denotes and
T6815 7679-7687 NN denotes pEGFP-C2
T6816 7688-7689 -LRB- denotes (
T6817 7689-7697 NNP denotes Clontech
T6818 7697-7698 -COMMA- denotes ,
T6819 7699-7720 NNP denotes Saint-Germain-en-Laye
T6820 7720-7721 -COMMA- denotes ,
T6821 7722-7728 NNP denotes France
T6822 7728-7729 -RRB- denotes )
T6823 7730-7733 VB denotes was
T6824 7734-7742 VB denotes digested
T6825 7743-7747 IN denotes with
T6826 7748-7753 NN denotes BamHI
T6827 7755-7758 NN denotes DNA
T6828 7759-7762 VB denotes was
T6829 7763-7772 VB denotes recovered
T6830 7773-7775 IN denotes by
T6831 7776-7782 NN denotes phenol
T6832 7783-7793 NN denotes extraction
T6833 7794-7797 CC denotes and
T6834 7798-7805 NN denotes ethanol
T6835 7806-7819 NN denotes precipitation
T6836 7819-7820 -COMMA- denotes ,
T6837 7821-7824 CC denotes and
T6838 7825-7829 CC denotes both
T6839 7830-7833 DT denotes the
T6840 7834-7840 NN denotes GATA-3
T6841 7841-7849 NN denotes fragment
T6842 7850-7853 CC denotes and
T6843 7854-7857 DT denotes the
T6844 7858-7866 NN denotes pEGFP-C2
T6845 7867-7873 NN denotes vector
T6846 7874-7885 JJ denotes blunt-ended
T6847 7886-7888 IN denotes by
T6848 7889-7899 NN denotes incubation
T6849 7900-7904 IN denotes with
T6850 7905-7911 NNP denotes Klenow
T6851 7912-7913 -LRB- denotes (
T6852 7913-7920 NN denotes Bioline
T6853 7921-7930 NN denotes Bio-27029
T6854 7930-7931 -RRB- denotes )
T6855 7932-7935 IN denotes for
T6856 7936-7938 CD denotes 30
T6857 7939-7942 NN denotes min
T6858 7943-7945 IN denotes at
T6859 7946-7951 NNP denotes 37°C.
T6860 7952-7958 NNP denotes Klenow
T6861 7959-7962 VB denotes was
T6862 7963-7974 VB denotes inactivated
T6863 7975-7977 IN denotes by
T6864 7978-7988 NN denotes incubation
T6865 7989-7992 IN denotes for
T6866 7993-7995 CD denotes 10
T6867 7996-7999 NN denotes min
T6868 8000-8002 IN denotes at
T6869 8003-8008 NNP denotes 75°C.
T6870 8009-8012 NN denotes DNA
T6871 8013-8016 VB denotes was
T6872 8017-8026 VB denotes recovered
T6873 8027-8029 IN denotes by
T6874 8030-8036 NN denotes phenol
T6875 8037-8047 NN denotes extraction
T6876 8048-8051 CC denotes and
T6877 8052-8059 NN denotes ethanol
T6878 8060-8073 NN denotes precipitation
T6879 8073-8074 -COMMA- denotes ,
T6880 8075-8078 CC denotes and
T6881 8079-8083 CC denotes both
T6882 8084-8087 DT denotes the
T6883 8088-8099 JJ denotes blunt-ended
T6884 8100-8106 NN denotes GATA-3
T6885 8107-8115 NN denotes fragment
T6886 8116-8119 CC denotes and
T6887 8120-8123 DT denotes the
T6888 8124-8127 NN denotes GFP
T6889 8128-8134 NN denotes vector
T6890 8135-8139 VB denotes were
T6891 8140-8152 RB denotes subsequently
T6892 8153-8161 VB denotes digested
T6893 8162-8166 IN denotes with
T6894 8167-8172 NN denotes EcoRI
T6895 8173-8179 IN denotes before
T6896 8180-8183 DT denotes the
T6897 8184-8201 JJ denotes 5′-EcoRI/3′–blunt
T6898 8202-8205 NN denotes end
T6899 8206-8212 NN denotes GATA-3
T6900 8213-8221 NN denotes fragment
T6901 8222-8225 VB denotes was
T6902 8226-8234 VB denotes inserted
T6903 8235-8239 IN denotes into
T6904 8240-8243 DT denotes the
T6905 8244-8261 NN denotes 5′-EcoRI/3′–blunt
T6906 8262-8267 VB denotes ended
T6907 8268-8271 NN denotes GFP
T6908 8272-8278 NN denotes vector
T6909 8280-8288 JJ denotes Positive
T6910 8289-8295 NN denotes clones
T6911 8296-8300 VB denotes were
T6912 8301-8310 VB denotes confirmed
T6913 8311-8313 IN denotes by
T6914 8314-8323 NN denotes digestion
T6915 8324-8327 CC denotes and
T6916 8328-8332 NN denotes size
T6917 8333-8341 NN denotes analysis
T6918 8342-8344 IN denotes by
T6919 8345-8346 CD denotes 1
T6920 8346-8347 NN denotes %
T6921 8348-8355 NN denotes agarose
T6922 8356-8359 NN denotes gel
T6923 8360-8375 NN denotes electrophoresis
T6924 8376-8379 CC denotes and
T6925 8380-8382 IN denotes by
T6926 8383-8393 NN denotes sequencing
T7172 8396-8408 NN denotes Transfection
T7173 8409-8415 NN denotes HuT-78
T7174 8416-8421 NN denotes cells
T7175 8422-8426 VB denotes were
T7176 8427-8438 VB denotes transfected
T7177 8439-8443 IN denotes with
T7178 8444-8450 CC denotes either
T7179 8451-8454 NN denotes EP8
T7180 8455-8457 CC denotes or
T7181 8458-8461 NN denotes GFP
T7182 8462-8468 NN denotes vector
T7183 8469-8473 RB denotes only
T7184 8474-8477 NN denotes DNA
T7185 8478-8483 VB denotes using
T7186 8484-8492 NN denotes solution
T7187 8493-8494 NNP denotes R
T7188 8494-8495 -COMMA- denotes ,
T7189 8496-8505 NN denotes programme
T7190 8506-8511 NN denotes V-001
T7191 8512-8514 IN denotes at
T7192 8515-8516 DT denotes a
T7193 8517-8522 NN denotes ratio
T7194 8523-8525 IN denotes of
T7195 8526-8531 CD denotes 3×106
T7196 8532-8539 NN denotes cells/4
T7197 8540-8542 VB denotes µg
T7198 8543-8546 NN denotes DNA
T7199 8547-8550 IN denotes for
T7200 8551-8554 CD denotes 7–8
T7201 8555-8556 NN denotes h
T7202 8557-8559 IN denotes in
T7203 8560-8568 JJ denotes complete
T7204 8569-8575 NN denotes medium
T7205 8576-8577 -LRB- denotes (
T7206 8577-8579 CD denotes 10
T7207 8579-8580 NN denotes %
T7208 8581-8587 JJ denotes bovine
T7209 8588-8593 NN denotes serum
T7210 8594-8596 IN denotes in
T7211 8597-8608 NN denotes RPMI1640+15
T7212 8609-8620 NN denotes L-glutamine
T7213 8620-8621 -RRB- denotes )
T7214 8622-8631 VB denotes according
T7215 8632-8634 TO denotes to
T7216 8635-8638 DT denotes the
T7217 8639-8646 JJ denotes general
T7218 8647-8652 NNP denotes Amaxa
T7219 8653-8661 NN denotes protocol
T7220 8662-8665 IN denotes for
T7221 8666-8679 NN denotes nucleofection
T7222 8681-8684 DT denotes The
T7223 8685-8691 NN denotes medium
T7224 8692-8695 VB denotes was
T7225 8696-8708 RB denotes subsequently
T7226 8709-8716 VB denotes changed
T7227 8717-8719 TO denotes to
T7228 8720-8721 CD denotes 1
T7229 8721-8722 NN denotes %
T7230 8723-8727 NN denotes RPMI
T7231 8728-8731 IN denotes for
T7232 8732-8734 CD denotes 24
T7233 8735-8736 NN denotes h
T7234 8737-8743 IN denotes before
T7235 8744-8755 VB denotes transfected
T7236 8756-8761 NN denotes cells
T7237 8762-8766 VB denotes were
T7238 8767-8772 VB denotes added
T7239 8773-8775 TO denotes to
T7240 8776-8789 JJ denotes anti-CD3/CD28
T7241 8790-8797 VB denotes treated
T7242 8798-8803 NN denotes wells
T7243 8804-8807 CC denotes and
T7244 8808-8812 JJ denotes live
T7245 8813-8817 NN denotes cell
T7246 8818-8833 NN denotes videomicroscopy
T7247 8834-8843 VB denotes performed
T7357 8846-8856 NNP denotes Time-Lapse
T7358 8857-8867 NNP denotes Microscopy
T7359 8868-8874 NN denotes HuT-78
T7360 8875-8880 NN denotes cells
T7361 8881-8891 VB denotes expressing
T7362 8892-8902 NN denotes GATA-3-GFP
T7363 8903-8907 VB denotes were
T7364 8908-8918 VB denotes maintained
T7365 8919-8921 IN denotes at
T7366 8922-8926 NN denotes 37°C
T7367 8927-8929 IN denotes in
T7368 8930-8936 NN denotes growth
T7369 8937-8943 NN denotes medium
T7370 8944-8946 IN denotes in
T7371 8947-8948 DT denotes a
T7372 8949-8955 JJ denotes closed
T7373 8956-8960 NN denotes FCS2
T7374 8961-8970 NN denotes perfusion
T7375 8971-8978 NN denotes chamber
T7376 8979-8980 -LRB- denotes (
T7377 8980-8989 NNP denotes Bioptechs
T7378 8989-8990 -COMMA- denotes ,
T7379 8991-8997 NNP denotes Butler
T7380 8997-8998 -COMMA- denotes ,
T7381 8999-9011 NNP denotes Pennsylvania
T7382 9011-9012 -COMMA- denotes ,
T7383 9013-9016 NNP denotes USA
T7384 9016-9017 -RRB- denotes )
T7385 9018-9026 VB denotes combined
T7386 9027-9031 IN denotes with
T7387 9032-9034 DT denotes an
T7388 9035-9044 JJ denotes objective
T7389 9045-9051 NN denotes heater
T7390 9052-9053 -LRB- denotes (
T7391 9053-9062 NN denotes Bioptechs
T7392 9062-9063 -RRB- denotes )
T7393 9064-9066 IN denotes on
T7394 9067-9070 DT denotes the
T7395 9071-9076 NN denotes stage
T7396 9077-9078 DT denotes a
T7397 9079-9084 NNP denotes Zeiss
T7398 9085-9093 NNP denotes Axiovert
T7399 9094-9097 CD denotes 200
T7400 9098-9108 NN denotes microscope
T7401 9109-9110 -LRB- denotes (
T7402 9110-9119 NNP denotes Thornwood
T7403 9119-9120 -COMMA- denotes ,
T7404 9121-9124 NNP denotes New
T7405 9125-9129 NNP denotes York
T7406 9129-9130 -COMMA- denotes ,
T7407 9131-9134 NNP denotes USA
T7408 9134-9135 -RRB- denotes )
T7409 9137-9149 NN denotes Observations
T7410 9150-9154 VB denotes were
T7411 9155-9159 VB denotes made
T7412 9160-9162 IN denotes by
T7413 9163-9169 CD denotes 40×1.0
T7414 9170-9172 NN denotes NA
T7415 9173-9186 NN denotes oil-immersion
T7416 9187-9196 JJ denotes objective
T7417 9197-9201 NN denotes lens
T7418 9201-9202 -COMMA- denotes ,
T7419 9203-9206 CC denotes and
T7420 9207-9219 NN denotes fluorescence
T7421 9220-9223 CC denotes and
T7422 9224-9229 NN denotes phase
T7423 9230-9238 NN denotes contrast
T7424 9239-9245 NN denotes images
T7425 9246-9250 VB denotes were
T7426 9251-9259 VB denotes gathered
T7427 9260-9265 VB denotes using
T7428 9266-9267 DT denotes a
T7429 9268-9277 NNP denotes Hamamatsu
T7430 9278-9285 NNP denotes ORCA-ER
T7431 9286-9293 VB denotes charged
T7432 9294-9301 VB denotes coupled
T7433 9302-9308 NN denotes device
T7434 9309-9315 NN denotes camera
T7435 9316-9317 -LRB- denotes (
T7436 9317-9328 NNP denotes Bridgewater
T7437 9328-9329 -COMMA- denotes ,
T7438 9330-9333 NNP denotes New
T7439 9334-9340 NNP denotes Jersey
T7440 9340-9341 -COMMA- denotes ,
T7441 9342-9345 NNP denotes USA
T7442 9345-9346 -RRB- denotes )
T7443 9347-9353 VB denotes driven
T7444 9354-9356 IN denotes by
T7445 9357-9364 NN denotes Openlab
T7446 9365-9373 NN denotes software
T7447 9374-9375 -LRB- denotes (
T7448 9375-9386 NNP denotes Improvision
T7449 9386-9387 -COMMA- denotes ,
T7450 9388-9396 NNP denotes Coventry
T7451 9396-9397 -COMMA- denotes ,
T7452 9398-9400 NNP denotes UK
T7453 9400-9401 -RRB- denotes )
T7454 9403-9414 NN denotes Photographs
T7455 9415-9419 VB denotes were
T7456 9420-9425 VB denotes taken
T7457 9426-9428 IN denotes at
T7458 9429-9430 CD denotes 0
T7459 9430-9431 -COMMA- denotes ,
T7460 9432-9434 CD denotes 30
T7461 9434-9435 -COMMA- denotes ,
T7462 9436-9438 CD denotes 60
T7463 9438-9439 -COMMA- denotes ,
T7464 9440-9443 CD denotes 120
T7465 9443-9444 -COMMA- denotes ,
T7466 9445-9448 CC denotes and
T7467 9449-9452 CD denotes 240
T7468 9453-9456 NN denotes min
T7611 9459-9472 JJ denotes Densitometric
T7612 9473-9481 NN denotes Analysis
T7613 9482-9494 NN denotes Densitometry
T7614 9495-9497 IN denotes of
T7615 9498-9501 NN denotes ECL
T7616 9502-9513 NN denotes immunoblots
T7617 9514-9517 VB denotes was
T7618 9518-9527 VB denotes performed
T7619 9528-9533 VB denotes using
T7620 9534-9542 NNP denotes Gelworks
T7621 9543-9545 NNP denotes ID
T7622 9546-9558 JJ denotes intermediate
T7623 9559-9567 NN denotes software
T7624 9568-9569 -LRB- denotes (
T7625 9569-9580 NNP denotes Ultraviolet
T7626 9581-9589 NNP denotes Products
T7627 9589-9590 -COMMA- denotes ,
T7628 9591-9605 NNP denotes Cambridgeshire
T7629 9605-9606 -COMMA- denotes ,
T7630 9607-9609 NNP denotes UK
T7631 9609-9610 -RRB- denotes )
T7632 9612-9619 RB denotes Briefly
T7633 9619-9620 -COMMA- denotes ,
T7634 9621-9632 NN denotes immunoblots
T7635 9633-9637 VB denotes were
T7636 9638-9645 VB denotes scanned
T7637 9646-9649 CC denotes and
T7638 9650-9655 NN denotes gates
T7639 9656-9660 VB denotes were
T7640 9661-9666 VB denotes drawn
T7641 9667-9674 RB denotes tightly
T7642 9675-9681 IN denotes around
T7643 9682-9686 DT denotes each
T7644 9687-9691 NN denotes band
T7645 9693-9703 NN denotes Background
T7646 9704-9710 NN denotes values
T7647 9711-9715 IN denotes from
T7648 9716-9720 DT denotes each
T7649 9721-9725 NN denotes lane
T7650 9726-9730 VB denotes were
T7651 9731-9741 VB denotes subtracted
T7652 9742-9744 TO denotes to
T7653 9745-9754 VB denotes normalize
T7654 9755-9759 DT denotes each
T7655 9760-9771 NN denotes measurement
T7656 9773-9776 DT denotes The
T7657 9777-9782 NN denotes bands
T7658 9783-9787 VB denotes were
T7659 9788-9798 VB denotes quantified
T7660 9799-9804 VB denotes using
T7661 9805-9808 DT denotes the
T7662 9809-9817 NNP denotes Gelworks
T7663 9818-9826 NN denotes software
T7664 9828-9831 DT denotes All
T7665 9832-9839 JJ denotes Western
T7666 9840-9845 NN denotes blots
T7667 9846-9850 VB denotes were
T7668 9851-9858 VB denotes exposed
T7669 9859-9861 TO denotes to
T7670 9862-9866 VB denotes film
T7671 9867-9870 IN denotes for
T7672 9871-9878 VB denotes varying
T7673 9879-9886 NN denotes lengths
T7674 9887-9889 IN denotes of
T7675 9890-9894 NN denotes time
T7676 9894-9895 -COMMA- denotes ,
T7677 9896-9899 CC denotes and
T7678 9900-9904 RB denotes only
T7679 9905-9910 NN denotes films
T7680 9911-9921 VB denotes generating
T7681 9922-9935 JJ denotes subsaturating
T7682 9936-9942 NN denotes levels
T7683 9943-9945 IN denotes of
T7684 9946-9955 NN denotes intensity
T7685 9956-9960 VB denotes were
T7686 9961-9969 VB denotes selected
T7687 9970-9973 IN denotes for
T7688 9974-9987 JJ denotes densitometric
T7689 9988-9998 NN denotes evaluation
T7787 10001-10012 JJ denotes Statistical
T7788 10013-10021 NN denotes Analysis
T7789 10022-10026 NN denotes Data
T7790 10027-10031 IN denotes from
T7791 10032-10037 CD denotes three
T7792 10038-10040 CC denotes or
T7793 10041-10045 JJ denotes more
T7794 10046-10057 JJ denotes independent
T7795 10058-10069 NN denotes experiments
T7796 10070-10073 VB denotes are
T7797 10074-10083 VB denotes presented
T7798 10084-10086 IN denotes as
T7799 10087-10090 DT denotes the
T7800 10091-10104 NN denotes mean±standard
T7801 10105-10110 NN denotes error
T7802 10111-10113 IN denotes of
T7803 10114-10117 DT denotes the
T7804 10118-10122 JJ denotes mean
T7805 10123-10124 -LRB- denotes (
T7806 10124-10127 NN denotes SEM
T7807 10127-10128 -RRB- denotes )
T7808 10128-10129 -COMMA- denotes ,
T7809 10130-10136 IN denotes except
T7810 10137-10142 WRB denotes where
T7811 10143-10149 VB denotes stated
T7812 10150-10153 CC denotes and
T7813 10154-10158 VB denotes were
T7814 10159-10167 VB denotes compared
T7815 10168-10173 VB denotes using
T7816 10174-10182 NNP denotes GraphPad
T7817 10183-10188 NNP denotes Prism
T7818 10189-10190 CD denotes 4
T7819 10191-10192 -LRB- denotes (
T7820 10192-10200 NNP denotes GraphPad
T7821 10201-10209 NNP denotes Software
T7822 10209-10210 -COMMA- denotes ,
T7823 10211-10215 NN denotes http
T7824 10215-10216 -COLON- denotes :
T7825 10216-10234 LS denotes //www.graphpad.com
T7826 10234-10235 -RRB- denotes )
T7827 10237-10244 NN denotes Results
T7828 10245-10249 VB denotes were
T7829 10250-10258 VB denotes analysed
T7830 10259-10264 VB denotes using
T7831 10265-10272 JJ denotes one-way
T7832 10273-10278 NN denotes ANOVA
T7833 10279-10283 IN denotes with
T7834 10284-10296 NN denotes Newman-Keuls
T7835 10297-10301 NN denotes post
T7836 10302-10306 NN denotes test
T7837 10307-10313 IN denotes except
T7838 10314-10317 IN denotes for
T7839 10318-10321 DT denotes the
T7840 10322-10326 NN denotes data
T7841 10327-10331 IN denotes from
T7842 10332-10335 DT denotes the
T7843 10336-10338 FW denotes in
T7844 10339-10343 FW denotes vivo
T7845 10344-10351 VB denotes inhaled
T7846 10352-10354 NN denotes FP
T7847 10355-10360 NN denotes study
T7848 10360-10361 -COMMA- denotes ,
T7849 10362-10367 WDT denotes which
T7850 10368-10371 VB denotes was
T7851 10372-10380 VB denotes analysed
T7852 10381-10383 IN denotes by
T7853 10384-10392 NNP denotes Friedman
T7854 10392-10394 POS denotes 's
T7855 10395-10399 NN denotes test
T7856 10400-10404 IN denotes with
T7857 10405-10415 JJ denotes subsequent
T7858 10416-10425 NNP denotes Wilcoxson
T7859 10426-10433 VB denotes matched
T7860 10434-10438 NN denotes pair
T7861 10439-10445 VB denotes signed
T7862 10446-10450 NN denotes rank
T7863 10451-10454 NN denotes sum
T7864 10455-10459 NN denotes test
T7865 10461-10465 NN denotes Data
T7866 10466-10470 IN denotes from
T7867 10471-10475 DT denotes this
T7868 10476-10484 NN denotes analysis
T7869 10485-10488 VB denotes are
T7870 10489-10498 VB denotes presented
T7871 10499-10501 IN denotes as
T7872 10502-10503 DT denotes a
T7873 10504-10520 NN denotes box-and-whiskers
T7874 10521-10525 NN denotes plot
T7875 10527-10535 NNP denotes Friedman
T7876 10535-10537 POS denotes 's
T7877 10538-10542 NN denotes test
T7878 10543-10546 VB denotes was
T7879 10547-10551 VB denotes used
T7880 10552-10554 IN denotes as
T7881 10555-10560 CD denotes three
T7882 10561-10568 JJ denotes matched
T7883 10569-10577 NN denotes measures
T7884 10578-10582 VB denotes were
T7885 10583-10591 VB denotes obtained
T7886 10592-10597 VB denotes using
T7887 10598-10605 NN denotes placebo
T7888 10605-10606 -COMMA- denotes ,
T7889 10607-10610 CD denotes 100
T7890 10611-10613 NN denotes µg
T7891 10613-10614 -COMMA- denotes ,
T7892 10615-10618 CC denotes and
T7893 10619-10622 CD denotes 500
T7894 10623-10625 NN denotes µg
T7895 10626-10628 IN denotes of
T7896 10629-10636 VB denotes inhaled
T7897 10637-10639 NN denotes FP
T7898 10639-10640 -COMMA- denotes ,
T7899 10641-10646 WDT denotes which
T7900 10647-10650 VB denotes had
T7901 10651-10659 JJ denotes variable
T7902 10660-10668 NN denotes baseline
T7903 10669-10675 NN denotes levels
T7904 10677-10679 PRP denotes We
T7905 10680-10683 VB denotes did
T7906 10684-10687 RB denotes not
T7907 10688-10694 VB denotes assume
T7908 10695-10696 DT denotes a
T7909 10697-10705 JJ denotes Gaussian
T7910 10706-10718 NN denotes distribution
T7911 10719-10721 IN denotes of
T7912 10722-10725 DT denotes the
T7913 10726-10730 NN denotes data
T7914 10731-10734 JJ denotes due
T7915 10735-10737 TO denotes to
T7916 10738-10741 DT denotes the
T7917 10742-10749 JJ denotes limited
T7918 10750-10757 NN denotes numbers
T7919 10758-10760 IN denotes of
T7920 10761-10773 NN denotes participants
T7921 10774-10782 VB denotes analysed
T7922 10783-10784 -LRB- denotes (
T7923 10784-10789 CD denotes seven
T7924 10789-10790 -RRB- denotes )
T7925 10790-10794 NN denotes .The
T7926 10795-10799 JJ denotes null
T7927 10800-10810 NN denotes hypothesis
T7928 10811-10814 VB denotes was
T7929 10815-10823 VB denotes rejected
T7930 10824-10826 IN denotes at
T7931 10827-10828 NN denotes p
T7932 10828-10829 SYM denotes <
T7933 10829-10833 CD denotes 0.05
T5299 4293-4295 CD denotes 35
T5300 4296-4302 NN denotes cycles
R3148 T3675 T3676 arg1Of Participants,and
R3149 T3678 T3676 arg2Of Design,and
R3150 T3678 T3677 arg1Of Design,Study
R3151 T3679 T3680 arg1Of We,studied
R3152 T3680 T3701 arg1Of studied,","
R3153 T3680 T3708 arg1Of studied,[
R3154 T3681 T3680 arg2Of patients,studied
R3155 T3681 T3682 arg1Of patients,with
R3156 T3681 T3685 arg1Of patients,who
R3157 T3681 T3686 arg1Of patients,were
R3158 T3681 T3688 arg2Of patients,treated
R3159 T3684 T3682 arg2Of asthma,with
R3160 T3684 T3683 arg1Of asthma,mild
R3161 T3688 T3686 arg2Of treated,were
R3162 T3688 T3687 arg1Of treated,not
R3163 T3688 T3689 arg1Of treated,with
R3164 T3691 T3689 arg2Of corticosteroids,with
R3165 T3691 T3690 arg2Of corticosteroids,inhaled
R3166 T3691 T3692 arg1Of corticosteroids,who
R3167 T3691 T3693 arg1Of corticosteroids,had
R3168 T3691 T3694 arg1Of corticosteroids,been
R3169 T3691 T3695 arg2Of corticosteroids,included
R3170 T3695 T3693 arg2Of included,had
R3171 T3695 T3694 arg2Of included,been
R3172 T3695 T3696 arg1Of included,in
R3173 T3699 T3698 arg1Of reported,previously
R3174 T3700 T3696 arg2Of double-blind,in
R3175 T3700 T3697 arg1Of double-blind,a
R3176 T3700 T3699 arg1Of double-blind,reported
R3177 T3702 T3703 arg1Of placebo-controlled,","
R3178 T3704 T3703 arg2Of crossover,","
R3179 T3705 T3680 arg3Of study,studied
R3180 T3705 T3702 arg1Of study,placebo-controlled
R3181 T3705 T3704 arg1Of study,crossover
R3182 T3705 T3706 arg1Of study,with
R3183 T3707 T3706 arg2Of FP,with
R3184 T3709 T3708 arg2Of 34,[
R3185 T3710 T3708 arg3Of ],[
R3186 T3712 T3711 arg1Of patients,Seven
R3187 T3712 T3713 arg1Of patients,with
R3188 T3712 T3716 arg1Of patients,entered
R3189 T3712 T3720 arg1Of patients,were
R3190 T3712 T3721 arg2Of patients,randomized
R3191 T3712 T3723 arg1Of patients,receive
R3192 T3715 T3713 arg2Of asthma,with
R3193 T3715 T3714 arg1Of asthma,mild
R3194 T3716 T3719 arg1Of entered,and
R3195 T3718 T3716 arg2Of study,entered
R3196 T3718 T3717 arg1Of study,the
R3197 T3719 T3745 arg1Of and,and
R3198 T3721 T3719 arg2Of randomized,and
R3199 T3721 T3720 arg2Of randomized,were
R3200 T3723 T3721 arg3Of receive,randomized
R3201 T3723 T3722 arg1Of receive,to
R3202 T3723 T3740 arg1Of receive,via
R3203 T3726 T3724 arg1Of inhalation,a
R3204 T3726 T3725 arg1Of inhalation,single
R3205 T3726 T3727 arg1Of inhalation,of
R3206 T3726 T3735 arg1Of inhalation,or
R3207 T3728 T3727 arg2Of FP,of
R3208 T3728 T3729 arg1Of FP,(
R3209 T3730 T3731 arg1Of 100,and
R3210 T3732 T3731 arg2Of 500,and
R3211 T3733 T3729 arg2Of µg,(
R3212 T3733 T3730 arg1Of µg,100
R3213 T3733 T3732 arg1Of µg,500
R3214 T3734 T3729 arg3Of ),(
R3215 T3735 T3723 arg2Of or,receive
R3216 T3739 T3735 arg2Of control,or
R3217 T3739 T3736 arg1Of control,a
R3218 T3739 T3737 arg1Of control,matched
R3219 T3739 T3738 arg1Of control,placebo
R3220 T3743 T3740 arg2Of chamber,via
R3221 T3743 T3741 arg1Of chamber,a
R3222 T3743 T3742 arg1Of chamber,spacer
R3223 T3745 T3744 arg1Of and,","
R3224 T3748 T3746 arg1Of treatment,the
R3225 T3748 T3747 arg1Of treatment,other
R3226 T3748 T3749 arg1Of treatment,was
R3227 T3748 T3750 arg2Of treatment,given
R3228 T3750 T3745 arg2Of given,and
R3229 T3750 T3749 arg2Of given,was
R3230 T3750 T3751 arg1Of given,after
R3231 T3754 T3751 arg2Of period,after
R3232 T3754 T3752 arg1Of period,a
R3233 T3754 T3753 arg1Of period,wash-out
R3234 T3754 T3755 arg1Of period,of
R3235 T3758 T3756 arg1Of 6,at
R3236 T3758 T3757 arg1Of 6,least
R3237 T3759 T3755 arg2Of d,of
R3238 T3759 T3758 arg1Of d,6
R3239 T3760 T3761 arg1Of Blood,was
R3240 T3760 T3762 arg2Of Blood,taken
R3241 T3762 T3761 arg2Of taken,was
R3242 T3762 T3763 arg1Of taken,for
R3243 T3764 T3763 arg2Of preparation,for
R3244 T3764 T3765 arg1Of preparation,of
R3245 T3764 T3767 arg1Of preparation,at
R3246 T3766 T3765 arg2Of PBMCs,of
R3247 T3768 T3769 arg1Of 1,and
R3248 T3770 T3769 arg2Of 2,and
R3249 T3771 T3767 arg2Of h,at
R3250 T3771 T3768 arg1Of h,1
R3251 T3771 T3770 arg1Of h,2
R3252 T3771 T3772 arg1Of h,after
R3253 T3774 T3772 arg2Of administration,after
R3254 T3774 T3773 arg1Of administration,drug
R3255 T3776 T3775 arg1Of patients,All
R3256 T3776 T3777 arg1Of patients,gave
R3257 T3776 T3801 arg1Of patients,was
R3258 T3776 T3802 arg2Of patients,conducted
R3259 T3777 T3780 arg1Of gave,and
R3260 T3779 T3777 arg2Of consent,gave
R3261 T3779 T3778 arg2Of consent,informed
R3262 T3782 T3781 arg1Of study,the
R3263 T3782 T3783 arg1Of study,was
R3264 T3782 T3784 arg2Of study,approved
R3265 T3784 T3783 arg2Of approved,was
R3266 T3788 T3786 arg1Of Committee,the
R3267 T3788 T3787 arg1Of Committee,Ethics
R3268 T3788 T3789 arg1Of Committee,of
R3269 T3788 T3793 arg1Of Committee,and
R3270 T3792 T3789 arg2Of Brompton,of
R3271 T3792 T3790 arg1Of Brompton,the
R3272 T3792 T3791 arg1Of Brompton,Royal
R3273 T3793 T3784 arg1Of and,approved
R3274 T3793 T3785 arg2Of and,by
R3275 T3797 T3794 arg1Of Trust.,Harefield
R3276 T3797 T3795 arg1Of Trust.,Hospitals
R3277 T3797 T3796 arg1Of Trust.,NHS
R3278 T3800 T3793 arg2Of study,and
R3279 T3800 T3797 arg1Of study,Trust.
R3280 T3800 T3798 arg1Of study,The
R3281 T3800 T3799 arg1Of study,clinical
R3282 T3802 T3780 arg2Of conducted,and
R3283 T3802 T3783 modOf conducted,was
R3284 T3802 T3801 arg2Of conducted,was
R3285 T3802 T3803 arg1Of conducted,before
R3286 T3805 T3803 arg2Of requirement,before
R3287 T3805 T3804 arg1Of requirement,the
R3288 T3805 T3806 arg1Of requirement,for
R3289 T3809 T3806 arg2Of Registration,for
R3290 T3809 T3807 arg1Of Registration,Clinical
R3291 T3809 T3808 arg1Of Registration,Trial
R3431 T3981 T3982 arg1Of Antibodies,and
R3432 T3983 T3982 arg2Of Reagents,and
R3433 T3986 T3984 arg1Of antibodies,The
R3434 T3986 T3985 arg1Of antibodies,monoclonal
R3435 T3986 T3987 arg1Of antibodies,against
R3436 T3986 T3997 arg1Of antibodies,were
R3437 T3986 T3998 arg2Of antibodies,purchased
R3438 T3989 T3988 arg1Of CD3,human
R3439 T3989 T3990 arg1Of CD3,","
R3440 T3990 T3992 arg1Of ",",","
R3441 T3991 T3990 arg2Of CD28,","
R3442 T3992 T3995 arg1Of ",",and
R3443 T3993 T3992 arg2Of GR,","
R3444 T3995 T3987 arg2Of and,against
R3445 T3995 T3994 arg1Of and,","
R3446 T3996 T3995 arg2Of importin-α,and
R3447 T3998 T3997 arg2Of purchased,were
R3448 T3998 T3999 arg1Of purchased,from
R3449 T4001 T3999 arg2Of Biosciences,from
R3450 T4001 T4000 arg1Of Biosciences,BD
R3451 T4001 T4002 arg1Of Biosciences,(
R3452 T4003 T4002 arg2Of Oxford,(
R3453 T4003 T4004 arg1Of Oxford,","
R3454 T4006 T4004 arg2Of Kingdom,","
R3455 T4006 T4005 arg1Of Kingdom,United
R3456 T4007 T4002 arg3Of ),(
R3457 T4009 T4008 arg1Of antibodies,Rabbit
R3458 T4009 T4010 arg1Of antibodies,against
R3459 T4009 T4023 arg1Of antibodies,were
R3460 T4009 T4024 arg2Of antibodies,obtained
R3461 T4012 T4011 arg1Of GATA-3,human
R3462 T4012 T4013 arg1Of GATA-3,(
R3463 T4012 T4016 arg1Of GATA-3,and
R3464 T4014 T4013 arg2Of H-48,(
R3465 T4015 T4013 arg3Of ),(
R3466 T4016 T4010 arg2Of and,against
R3467 T4017 T4016 arg2Of GR,and
R3468 T4017 T4018 arg1Of GR,(
R3469 T4019 T4018 arg2Of E-20,(
R3470 T4019 T4020 arg1Of E-20,","
R3471 T4021 T4020 arg2Of sc-1003,","
R3472 T4022 T4018 arg3Of ),(
R3473 T4024 T4023 arg2Of obtained,were
R3474 T4024 T4025 arg1Of obtained,from
R3475 T4028 T4026 arg1Of Biotechnology,Santa
R3476 T4028 T4027 arg1Of Biotechnology,Cruz
R3477 T4028 T4029 arg1Of Biotechnology,(
R3478 T4028 T4038 arg1Of Biotechnology,","
R3479 T4031 T4030 arg1Of Cruz,Santa
R3480 T4031 T4032 arg1Of Cruz,","
R3481 T4032 T4034 arg1Of ",",","
R3482 T4033 T4032 arg2Of California,","
R3483 T4034 T4029 arg2Of ",",(
R3484 T4036 T4034 arg2Of States,","
R3485 T4036 T4035 arg1Of States,United
R3486 T4037 T4029 arg3Of ),(
R3487 T4038 T4046 arg1Of ",",and
R3488 T4041 T4038 arg2Of antibodies,","
R3489 T4041 T4039 arg1Of antibodies,polyclonal
R3490 T4041 T4040 arg1Of antibodies,rabbit
R3491 T4041 T4042 arg1Of antibodies,against
R3492 T4045 T4042 arg2Of kinase,against
R3493 T4045 T4043 arg1Of kinase,phospho-p38
R3494 T4045 T4044 arg1Of kinase,MAP
R3495 T4046 T4080 arg1Of and,and
R3496 T4047 T4046 arg2Of phospho-ATF-2,and
R3497 T4047 T4048 arg1Of phospho-ATF-2,from
R3498 T4051 T4049 arg1Of Technology,Cell
R3499 T4051 T4050 arg1Of Technology,Signaling
R3500 T4051 T4052 arg1Of Technology,(
R3501 T4051 T4061 arg1Of Technology,","
R3502 T4055 T4052 arg2Of Biolabs,(
R3503 T4055 T4053 arg1Of Biolabs,New
R3504 T4055 T4054 arg1Of Biolabs,England
R3505 T4055 T4056 arg1Of Biolabs,","
R3506 T4057 T4056 arg2Of Hertford,","
R3507 T4057 T4058 arg1Of Hertford,","
R3508 T4057 T4059 arg1Of Hertford,UK
R3509 T4060 T4052 arg3Of ),(
R3510 T4061 T4048 arg2Of ",",from
R3511 T4061 T4070 arg1Of ",",from
R3512 T4063 T4061 arg2Of antibody,","
R3513 T4063 T4062 arg1Of antibody,monoclonal
R3514 T4063 T4064 arg1Of antibody,against
R3515 T4065 T4064 arg2Of phosphoserine,against
R3516 T4065 T4066 arg1Of phosphoserine,(
R3517 T4068 T4066 arg2Of 4H4,(
R3518 T4068 T4067 arg1Of 4H4,clone
R3519 T4069 T4066 arg3Of ),(
R3520 T4073 T4070 arg2Of Products,from
R3521 T4073 T4071 arg1Of Products,Affiniti
R3522 T4073 T4072 arg1Of Products,Research
R3523 T4073 T4074 arg1Of Products,(
R3524 T4075 T4074 arg2Of Exeter,(
R3525 T4075 T4076 arg1Of Exeter,","
R3526 T4075 T4077 arg1Of Exeter,UK
R3527 T4078 T4074 arg3Of ),(
R3528 T4080 T4025 arg2Of and,from
R3529 T4080 T4079 arg1Of and,","
R3530 T4081 T4080 arg2Of antibodies,and
R3531 T4081 T4082 arg1Of antibodies,against
R3532 T4085 T4082 arg2Of TRITC,against
R3533 T4085 T4083 arg1Of TRITC,rabbit
R3534 T4085 T4084 arg1Of TRITC,IgG-conjugated
R3535 T4085 T4086 arg1Of TRITC,from
R3536 T4085 T4089 arg1Of TRITC,(
R3537 T4088 T4086 arg2Of Cytomation,from
R3538 T4088 T4087 arg1Of Cytomation,Dako
R3539 T4090 T4089 arg2Of Cambridge,(
R3540 T4090 T4091 arg1Of Cambridge,","
R3541 T4090 T4092 arg1Of Cambridge,UK
R3542 T4093 T4089 arg3Of ),(
R3543 T4096 T4094 arg1Of antibody,The
R3544 T4096 T4095 arg1Of antibody,anti-GR
R3545 T4096 T4097 arg1Of antibody,(
R3546 T4096 T4101 arg1Of antibody,from
R3547 T4096 T4110 arg1Of antibody,was
R3548 T4096 T4111 arg2Of antibody,used
R3549 T4098 T4097 arg2Of Clone,(
R3550 T4098 T4099 arg1Of Clone,41
R3551 T4100 T4097 arg3Of ),(
R3552 T4104 T4101 arg2Of Laboratories,from
R3553 T4104 T4102 arg1Of Laboratories,BD
R3554 T4104 T4103 arg1Of Laboratories,Transduction
R3555 T4104 T4105 arg1Of Laboratories,(
R3556 T4106 T4105 arg2Of Oxford,(
R3557 T4106 T4107 arg1Of Oxford,","
R3558 T4106 T4108 arg1Of Oxford,UK
R3559 T4109 T4105 arg3Of ),(
R3560 T4111 T4110 arg2Of used,was
R3561 T4111 T4112 arg1Of used,for
R3562 T4115 T4112 arg2Of analysis,for
R3563 T4115 T4113 arg1Of analysis,Western
R3564 T4115 T4114 arg1Of analysis,blot
R3565 T4118 T4116 arg1Of reagents,All
R3566 T4118 T4117 arg1Of reagents,other
R3567 T4118 T4119 arg1Of reagents,were
R3568 T4118 T4120 arg2Of reagents,purchased
R3569 T4120 T4119 arg2Of purchased,were
R3570 T4120 T4121 arg1Of purchased,from
R3571 T4122 T4121 arg2Of Sigma,from
R3572 T4122 T4123 arg1Of Sigma,(
R3573 T4124 T4123 arg2Of Poole,(
R3574 T4124 T4125 arg1Of Poole,","
R3575 T4126 T4125 arg2Of UK,","
R3576 T4127 T4123 arg3Of ),(
R3731 T4355 T4354 arg1Of Culture,Cell
R3732 T4355 T4356 arg1Of Culture,and
R3733 T4358 T4356 arg2Of Isolation,and
R3734 T4358 T4357 arg1Of Isolation,PBMC
R3735 T4363 T4359 arg1Of line,A
R3736 T4363 T4360 arg1Of line,human
R3737 T4363 T4361 arg1Of line,T
R3738 T4363 T4362 arg1Of line,cell
R3739 T4363 T4364 arg1Of line,(
R3740 T4363 T4367 arg1Of line,was
R3741 T4363 T4368 arg2Of line,purchased
R3742 T4365 T4364 arg2Of HuT-78,(
R3743 T4366 T4364 arg3Of ),(
R3744 T4368 T4367 arg2Of purchased,was
R3745 T4368 T4369 arg1Of purchased,from
R3746 T4372 T4370 arg1Of Collection,ECACC
R3747 T4372 T4371 arg1Of Collection,European
R3748 T4372 T4373 arg1Of Collection,of
R3749 T4372 T4376 arg1Of Collection,(
R3750 T4372 T4381 arg1Of Collection,were
R3751 T4372 T4382 arg2Of Collection,cultured
R3752 T4375 T4373 arg2Of Culture,of
R3753 T4375 T4374 arg1Of Culture,Cell
R3754 T4377 T4376 arg2Of Wiltshire,(
R3755 T4377 T4378 arg1Of Wiltshire,","
R3756 T4377 T4379 arg1Of Wiltshire,UK
R3757 T4380 T4376 arg3Of ),(
R3758 T4382 T4367 modOf cultured,was
R3759 T4382 T4381 arg2Of cultured,were
R3760 T4382 T4383 arg1Of cultured,as
R3761 T4385 T4384 arg1Of described,previously
R3762 T4387 T4383 arg2Of 12,as
R3763 T4387 T4385 arg1Of 12,described
R3764 T4387 T4386 arg2Of 12,[
R3765 T4388 T4386 arg3Of ],[
R3766 T4389 T4390 arg1Of PBMCs,were
R3767 T4389 T4391 arg2Of PBMCs,isolated
R3768 T4391 T4390 arg2Of isolated,were
R3769 T4391 T4392 arg1Of isolated,by
R3770 T4391 T4395 arg1Of isolated,over
R3771 T4394 T4392 arg2Of centrifugation,by
R3772 T4394 T4393 arg1Of centrifugation,density
R3773 T4396 T4395 arg2Of Ficoll-Hypaque,over
R3774 T4396 T4397 arg1Of Ficoll-Hypaque,(
R3775 T4396 T4410 arg1Of Ficoll-Hypaque,as
R3776 T4398 T4399 arg1Of density,","
R3777 T4398 T4402 arg1Of density,;
R3778 T4401 T4399 arg2Of g/ml,","
R3779 T4401 T4400 arg1Of g/ml,1.077
R3780 T4402 T4397 arg2Of ;,(
R3781 T4402 T4407 arg1Of ;,","
R3782 T4402 T4408 arg1Of ;,UK
R3783 T4404 T4402 arg2Of Biosciences,;
R3784 T4404 T4403 arg1Of Biosciences,Amersham
R3785 T4404 T4405 arg1Of Biosciences,","
R3786 T4406 T4405 arg2Of Amersham,","
R3787 T4409 T4397 arg3Of ),(
R3788 T4412 T4411 arg1Of described,previously
R3789 T4414 T4410 arg2Of 34,as
R3790 T4414 T4412 arg1Of 34,described
R3791 T4414 T4413 arg2Of 34,[
R3792 T4415 T4413 arg3Of ],[
R3793 T4416 T4417 arg1Of Cells,were
R3794 T4416 T4418 arg2Of Cells,stimulated
R3795 T4418 T4417 arg2Of stimulated,were
R3796 T4418 T4419 arg1Of stimulated,with
R3797 T4418 T4426 arg1Of stimulated,for
R3798 T4420 T4419 arg2Of anti-CD3/CD28,with
R3799 T4420 T4421 arg1Of anti-CD3/CD28,(
R3800 T4423 T4421 arg2Of µg/ml,(
R3801 T4423 T4422 arg1Of µg/ml,1
R3802 T4423 T4424 arg1Of µg/ml,each
R3803 T4425 T4421 arg3Of ),(
R3804 T4428 T4426 arg2Of h,for
R3805 T4428 T4427 arg1Of h,1
R3806 T4428 T4429 arg1Of h,at
R3807 T4428 T4431 modOf h,to
R3808 T4428 T4443 arg1Of h,(
R3809 T4430 T4429 arg2Of 37°C,at
R3810 T4432 T4431 arg1Of stimulate,to
R3811 T4435 T4432 arg2Of release,stimulate
R3812 T4435 T4433 arg1Of release,Th2
R3813 T4435 T4434 arg1Of release,cytokine
R3814 T4435 T4436 arg1Of release,in
R3815 T4435 T4441 arg1Of release,of
R3816 T4438 T4439 arg1Of presence,or
R3817 T4439 T4436 arg2Of or,in
R3818 T4439 T4437 arg1Of or,the
R3819 T4440 T4439 arg2Of absence,or
R3820 T4442 T4441 arg2Of FP,of
R3821 T4444 T4443 arg2Of 10−12,(
R3822 T4444 T4445 arg1Of 10−12,to
R3823 T4446 T4445 arg2Of 10−8M,to
R3824 T4447 T4443 arg3Of ),(
R3825 T4448 T4449 arg1Of Cytospins,were
R3826 T4448 T4450 arg2Of Cytospins,prepared
R3827 T4450 T4449 arg2Of prepared,were
R3828 T4450 T4451 arg1Of prepared,and
R3829 T4453 T4452 arg1Of localization,GATA-3
R3830 T4453 T4454 arg1Of localization,determined
R3831 T4454 T4451 arg2Of determined,and
R3832 T4457 T4454 arg2Of microscopy,determined
R3833 T4457 T4455 arg2Of microscopy,by
R3834 T4457 T4456 arg1Of microscopy,confocal
R3835 T4457 T4458 arg1Of microscopy,as
R3836 T4460 T4459 arg1Of described,previously
R3837 T4462 T4458 arg2Of 12,as
R3838 T4462 T4460 arg1Of 12,described
R3839 T4462 T4461 arg2Of 12,[
R3840 T4463 T4461 arg3Of ],[
R3969 T4624 T4622 arg1Of PCR,Reverse
R3970 T4624 T4623 arg1Of PCR,Transcription
R3971 T4626 T4625 arg1Of RNA,Total
R3972 T4626 T4627 arg1Of RNA,was
R3973 T4626 T4628 arg2Of RNA,extracted
R3975 T4628 T4627 arg2Of extracted,was
R3979 T4634 T4632 arg2Of kit,(
R3980 T4634 T4633 arg1Of kit,RNeasy
R3981 T4634 T4635 arg1Of kit,;
R3982 T4636 T4635 arg2Of Qiagen,;
R3983 T4636 T4637 arg1Of Qiagen,","
R3984 T4638 T4637 arg2Of Crawley,","
R3985 T4638 T4639 arg1Of Crawley,","
R3986 T4640 T4639 arg2Of UK,","
R3987 T4641 T4632 arg3Of ),(
R3988 T4644 T4642 arg2Of purification,During
R3989 T4644 T4643 arg1Of purification,RNA
R3990 T4647 T4646 arg1Of DNA,genomic
R3991 T4647 T4648 arg1Of DNA,was
R3992 T4647 T4649 arg2Of DNA,digested
R3993 T4649 T4642 arg1Of digested,During
R3994 T4649 T4645 arg1Of digested,","
R3995 T4649 T4648 arg2Of digested,was
R3996 T4649 T4650 arg1Of digested,with
R3997 T4652 T4650 arg2Of DNase,with
R3998 T4652 T4651 arg1Of DNase,RNase-free
R3999 T4652 T4653 arg1Of DNase,(
R4000 T4655 T4653 arg2Of Biosciences,(
R4001 T4655 T4654 arg1Of Biosciences,Amersham
R4002 T4656 T4653 arg3Of ),(
R4003 T4660 T4659 arg1Of µg,0.5
R4004 T4660 T4661 arg1Of µg,of
R4005 T4660 T4664 arg1Of µg,was
R4006 T4660 T4665 arg2Of µg,reversed
R4007 T4663 T4661 arg2Of RNA,of
R4008 T4663 T4662 arg1Of RNA,total
R4009 T4665 T4657 arg1Of reversed,Next
R4010 T4665 T4658 arg1Of reversed,","
R4011 T4665 T4664 arg2Of reversed,was
R4012 T4665 T4666 modOf reversed,transcribed
R4013 T4665 T4667 modOf reversed,using
R4014 T4665 T4673 arg1Of reversed,(
R4015 T4672 T4667 arg2Of RT,using
R4016 T4672 T4668 arg1Of RT,the
R4017 T4672 T4669 arg1Of RT,avian
R4018 T4672 T4670 arg1Of RT,myeloblastosis
R4019 T4672 T4671 arg1Of RT,virus
R4020 T4674 T4675 arg1Of Promega,","
R4021 T4675 T4673 arg2Of ",",(
R4022 T4675 T4677 arg1Of ",",","
R4023 T4676 T4675 arg2Of Southampton,","
R4024 T4678 T4677 arg2Of UK,","
R4025 T4679 T4673 arg3Of ),(
R4026 T4682 T4680 arg2Of quantification,For
R4027 T4682 T4681 arg1Of quantification,relative
R4028 T4684 T4685 arg1Of RT-PCR,was
R4029 T4684 T4686 arg2Of RT-PCR,carried
R4030 T4686 T4680 arg1Of carried,For
R4031 T4686 T4683 arg1Of carried,","
R4032 T4686 T4685 arg2Of carried,was
R4033 T4686 T4687 arg1Of carried,out
R4034 T4686 T4688 modOf carried,using
R4035 T4690 T4688 arg2Of probes,using
R4036 T4690 T4689 arg1Of probes,cDNA
R4037 T4691 T4692 arg1Of Primers,for
R4038 T4691 T4694 arg1Of Primers,were
R4039 T4691 T4695 arg1Of Primers,from
R4040 T4693 T4692 arg2Of IL-4,for
R4041 T4694 T4697 arg1Of were,(
R4042 T4695 T4694 arg2Of from,were
R4043 T4696 T4695 arg2Of Sigma-Genosys,from
R4044 T4698 T4697 arg2Of Cambridge,(
R4045 T4698 T4699 arg1Of Cambridge,","
R4046 T4698 T4700 arg1Of Cambridge,UK
R4047 T4701 T4697 arg3Of ),(
R4048 T4702 T4703 arg1Of Sequences,of
R4049 T4702 T4706 arg1Of Sequences,are
R4050 T4702 T4708 arg1Of Sequences,follows
R4051 T4704 T4703 arg2Of GADPH,of
R4052 T4704 T4705 arg2Of GADPH,used
R4053 T4706 T4707 arg1Of are,as
R4054 T4708 T4707 arg2Of follows,as
R4055 T4708 T4709 arg1Of follows,:
R4056 T4711 T4708 arg2Of 5′-CCACCCATGGCAAATTCCATGGC,follows
R4057 T4711 T4710 arg1Of 5′-CCACCCATGGCAAATTCCATGGC,forward
R4058 T4711 T4712 arg1Of 5′-CCACCCATGGCAAATTCCATGGC,","
R4059 T4714 T4712 arg2Of 3′-TCTAGACGGCAGGTCAGGTCCAC,","
R4060 T4714 T4713 arg1Of 3′-TCTAGACGGCAGGTCAGGTCCAC,reverse
R4166 T4854 T4853 arg1Of Fractionation,Cell
R4167 T4854 T4855 arg1Of Fractionation,","
R4168 T4855 T4858 arg1Of ",",and
R4169 T4856 T4855 arg2Of Immunoprecipitation,","
R4170 T4858 T4857 arg1Of and,","
R4171 T4860 T4859 arg1Of Blot,Western
R4172 T4861 T4858 arg2Of Analysis,and
R4173 T4861 T4860 arg1Of Analysis,Blot
R4174 T4862 T4863 arg1Of Nuclear,and
R4175 T4864 T4863 arg2Of cytoplasmic,and
R4176 T4865 T4862 arg1Of fractions,Nuclear
R4177 T4865 T4864 arg1Of fractions,cytoplasmic
R4178 T4865 T4866 arg1Of fractions,were
R4179 T4865 T4867 arg2Of fractions,prepared
R4180 T4867 T4866 arg2Of prepared,were
R4181 T4867 T4868 arg1Of prepared,as
R4182 T4870 T4869 arg1Of described,previously
R4183 T4872 T4868 arg2Of 35,as
R4184 T4872 T4870 arg1Of 35,described
R4185 T4872 T4871 arg2Of 35,[
R4186 T4873 T4871 arg3Of ],[
R4187 T4876 T4874 arg1Of lysates,Whole
R4188 T4876 T4875 arg1Of lysates,cell
R4189 T4876 T4877 arg1Of lysates,were
R4190 T4876 T4878 arg2Of lysates,prepared
R4191 T4878 T4877 arg2Of prepared,were
R4192 T4878 T4879 arg1Of prepared,in
R4193 T4882 T4879 arg2Of buffer,in
R4194 T4882 T4880 arg1Of buffer,NP-40
R4195 T4882 T4881 arg1Of buffer,lysis
R4196 T4882 T4883 arg1Of buffer,(
R4197 T4882 T4901 arg1Of buffer,in
R4198 T4884 T4885 arg1Of 0.5,%
R4199 T4887 T4884 arg1Of P-40,0.5
R4200 T4887 T4886 arg1Of P-40,Nonidet
R4201 T4887 T4888 arg1Of P-40,","
R4202 T4888 T4896 arg1Of ",",","
R4203 T4889 T4890 arg1Of 20,mM
R4204 T4891 T4888 arg2Of Tris-HCl,","
R4205 T4891 T4889 arg1Of Tris-HCl,20
R4206 T4891 T4892 arg1Of Tris-HCl,[
R4207 T4893 T4892 arg2Of pH,[
R4208 T4893 T4894 arg1Of pH,7.5
R4209 T4895 T4892 arg3Of ],[
R4210 T4896 T4883 arg2Of ",",(
R4211 T4897 T4898 arg1Of 150,mM
R4212 T4899 T4896 arg2Of NaCl,","
R4213 T4899 T4897 arg1Of NaCl,150
R4214 T4900 T4883 arg3Of ),(
R4215 T4903 T4901 arg2Of presence,in
R4216 T4903 T4902 arg1Of presence,the
R4217 T4903 T4904 arg1Of presence,of
R4218 T4908 T4904 arg2Of inhibitor,of
R4219 T4908 T4905 arg1Of inhibitor,complete
R4220 T4908 T4906 arg1Of inhibitor,protease
R4221 T4908 T4907 arg1Of inhibitor,cocktail
R4222 T4909 T4910 arg1Of Lysates,were
R4223 T4909 T4911 arg2Of Lysates,centrifuged
R4224 T4909 T4925 arg1Of Lysates,remove
R4225 T4911 T4910 arg2Of centrifuged,were
R4226 T4911 T4912 arg1Of centrifuged,at
R4227 T4911 T4914 arg1Of centrifuged,for
R4228 T4911 T4917 arg1Of centrifuged,at
R4229 T4911 T4920 arg1Of centrifuged,in
R4230 T4911 T4924 modOf centrifuged,to
R4231 T4913 T4912 arg2Of 4°C,at
R4232 T4916 T4914 arg2Of min,for
R4233 T4916 T4915 arg1Of min,10
R4234 T4919 T4917 arg2Of rpm,at
R4235 T4919 T4918 arg1Of rpm,"12,000"
R4236 T4923 T4920 arg2Of microcentrifuge,in
R4237 T4923 T4921 arg1Of microcentrifuge,an
R4238 T4923 T4922 arg1Of microcentrifuge,Eppendorf
R4239 T4925 T4924 arg1Of remove,to
R4240 T4927 T4925 arg2Of debris,remove
R4241 T4927 T4926 arg1Of debris,cellular
R4242 T4928 T4929 arg1Of Samples,were
R4243 T4928 T4931 arg2Of Samples,immunoprecipitated
R4244 T4931 T4929 arg2Of immunoprecipitated,were
R4245 T4931 T4930 arg1Of immunoprecipitated,then
R4246 T4931 T4932 arg1Of immunoprecipitated,with
R4247 T4935 T4932 arg2Of µl,with
R4248 T4935 T4933 arg1Of µl,either
R4249 T4935 T4934 arg1Of µl,10
R4250 T4935 T4936 arg1Of µl,of
R4251 T4935 T4942 arg1Of µl,using
R4252 T4935 T4952 arg1Of µl,using
R4253 T4935 T4958 arg1Of µl,(
R4254 T4937 T4936 arg2Of antibody,of
R4255 T4937 T4938 arg1Of antibody,against
R4256 T4939 T4940 arg1Of GATA-3,or
R4257 T4940 T4938 arg2Of or,against
R4258 T4941 T4940 arg2Of importin-α,or
R4259 T4942 T4946 arg1Of using,in
R4260 T4942 T4952 modOf using,using
R4261 T4945 T4942 arg2Of slurry,using
R4262 T4945 T4943 arg1Of slurry,A/G
R4263 T4945 T4944 arg2Of slurry,agarose
R4264 T4948 T4946 arg2Of presence,in
R4265 T4948 T4947 arg1Of presence,the
R4266 T4948 T4949 arg1Of presence,of
R4267 T4951 T4949 arg2Of inhibitor,of
R4268 T4951 T4950 arg1Of inhibitor,protease
R4269 T4954 T4955 arg1Of Catch,and
R4270 T4956 T4955 arg2Of Release,and
R4271 T4957 T4952 arg2Of methodology,using
R4272 T4957 T4953 arg1Of methodology,the
R4273 T4957 T4954 arg1Of methodology,Catch
R4274 T4957 T4956 arg1Of methodology,Release
R4275 T4960 T4959 arg1Of Biotechnology,Upstate
R4276 T4960 T4961 arg1Of Biotechnology,","
R4277 T4961 T4964 arg1Of ",",","
R4278 T4963 T4961 arg2Of Placid,","
R4279 T4963 T4962 arg1Of Placid,Lake
R4280 T4964 T4958 arg2Of ",",(
R4281 T4964 T4967 arg1Of ",",","
R4282 T4964 T4968 arg1Of ",",USA
R4283 T4966 T4964 arg2Of York,","
R4284 T4966 T4965 arg1Of York,New
R4285 T4969 T4958 arg3Of ),(
R4286 T4972 T4970 arg1Of analysis,Western
R4287 T4972 T4971 arg1Of analysis,blot
R4288 T4972 T4973 arg1Of analysis,was
R4289 T4972 T4974 arg2Of analysis,performed
R4290 T4974 T4973 arg2Of performed,was
R4291 T4975 T4974 arg3Of using,performed
R4292 T4976 T4975 arg2Of anti-GATA-3,using
R4293 T4976 T4977 arg1Of anti-GATA-3,","
R4294 T4978 T4979 arg1Of anti-importin-α,","
R4295 T4979 T4981 arg1Of ",",","
R4296 T4980 T4979 arg2Of anti-GR,","
R4297 T4982 T4981 arg2Of anti-p-p38,","
R4298 T4984 T4978 arg1Of kinase,anti-importin-α
R4299 T4984 T4980 arg1Of kinase,anti-GR
R4300 T4984 T4982 arg1Of kinase,anti-p-p38
R4301 T4984 T4983 arg1Of kinase,MAP
R4302 T4984 T4985 arg1Of kinase,","
R4303 T4985 T4988 arg1Of ",",and
R4304 T4986 T4985 arg2Of anti-p-ATF-2,","
R4305 T4988 T4977 arg2Of and,","
R4306 T4988 T4987 arg1Of and,","
R4307 T4989 T4988 arg2Of anti-p-serine,and
R4308 T4991 T4990 arg1Of proteins,Immunoreactive
R4309 T4991 T4992 arg1Of proteins,were
R4310 T4991 T4993 arg2Of proteins,detected
R4311 T4993 T4992 arg2Of detected,were
R4312 T4994 T4993 arg3Of using,detected
R4313 T4999 T4994 arg2Of kit,using
R4314 T4999 T4995 arg1Of kit,an
R4315 T4999 T4996 arg2Of kit,enhanced
R4316 T4999 T4997 arg1Of kit,chemiluminescence
R4317 T4999 T4998 arg1Of kit,ECL
R4318 T4999 T5000 arg1Of kit,(
R4319 T5002 T5000 arg2Of Biosciences,(
R4320 T5002 T5001 arg1Of Biosciences,Amersham
R4321 T5003 T5000 arg3Of ),(
R4483 T5207 T5206 arg1Of Immunoprecipitation,Chromatin
R4484 T5209 T5208 arg1Of immunoprecipitation,Chromatin
R4485 T5209 T5210 arg1Of immunoprecipitation,(
R4486 T5209 T5213 arg1Of immunoprecipitation,was
R4487 T5209 T5214 arg2Of immunoprecipitation,performed
R4488 T5211 T5210 arg2Of IP,(
R4489 T5212 T5210 arg3Of ),(
R4490 T5214 T5213 arg2Of performed,was
R4491 T5214 T5215 arg1Of performed,as
R4492 T5214 T5239 arg1Of performed,as
R4493 T5214 T5248 arg1Of performed,overnight
R4494 T5217 T5216 arg1Of described,previously
R4495 T5219 T5215 arg2Of 12,as
R4496 T5219 T5217 arg1Of 12,described
R4497 T5219 T5218 arg2Of 12,[
R4498 T5219 T5221 arg1Of 12,in
R4499 T5219 T5224 arg1Of 12,with
R4500 T5220 T5218 arg3Of ],[
R4501 T5223 T5221 arg2Of T-cells,in
R4502 T5223 T5222 arg1Of T-cells,HuT-78
R4503 T5226 T5224 arg2Of µg,with
R4504 T5226 T5225 arg1Of µg,2
R4505 T5226 T5227 arg1Of µg,of
R4506 T5228 T5229 arg1Of anti-GATA-3,(
R4507 T5228 T5235 arg1Of anti-GATA-3,or
R4508 T5232 T5229 arg2Of Biotechnology,(
R4509 T5232 T5230 arg1Of Biotechnology,Santa
R4510 T5232 T5231 arg1Of Biotechnology,Cruz
R4511 T5233 T5229 arg3Of ),(
R4512 T5235 T5227 arg2Of or,of
R4513 T5235 T5234 arg1Of or,","
R4514 T5238 T5235 arg2Of G,or
R4515 T5238 T5236 arg1Of G,isotypic
R4516 T5238 T5237 arg1Of G,immunoglobulin
R4517 T5242 T5239 arg2Of control,as
R4518 T5242 T5240 arg1Of control,a
R4519 T5242 T5241 arg1Of control,non-specific
R4520 T5242 T5243 arg1Of control,(
R4521 T5246 T5243 arg2Of Biotechnology,(
R4522 T5246 T5244 arg1Of Biotechnology,Santa
R4523 T5246 T5245 arg1Of Biotechnology,Cruz
R4524 T5247 T5243 arg3Of ),(
R4525 T5251 T5250 arg1Of Promoter,4°C.
R4526 T5252 T5251 arg1Of sequences,Promoter
R4527 T5252 T5253 arg1Of sequences,were
R4528 T5252 T5254 arg2Of sequences,detected
R4529 T5252 T5271 arg1Of sequences,reverse
R4530 T5254 T5253 arg2Of detected,were
R4531 T5254 T5255 arg1Of detected,with
R4532 T5254 T5270 arg1Of detected,and
R4533 T5257 T5255 arg2Of primers,with
R4534 T5257 T5256 arg1Of primers,PCR
R4535 T5257 T5258 arg1Of primers,for
R4536 T5261 T5259 arg1Of promoter,the
R4537 T5261 T5260 arg1Of promoter,IL-5
R4538 T5261 T5262 arg1Of promoter,(
R4539 T5261 T5267 arg1Of promoter,:
R4540 T5265 T5262 arg2Of +4,(
R4541 T5265 T5263 arg1Of +4,−445
R4542 T5265 T5264 arg1Of +4,to
R4543 T5266 T5262 arg3Of ),(
R4544 T5269 T5258 arg2Of 5′-TTAATCTAGCCACAGTCATAG-3′,for
R4545 T5269 T5261 arg1Of 5′-TTAATCTAGCCACAGTCATAG-3′,promoter
R4546 T5269 T5268 arg1Of 5′-TTAATCTAGCCACAGTCATAG-3′,forward
R4547 T5270 T5213 modOf and,was
R4548 T5270 T5249 arg1Of and,at
R4549 T5270 T5272 arg1Of and,:
R4550 T5271 T5270 arg2Of reverse,and
R4551 T5274 T5275 arg1Of PCR,was
R4552 T5274 T5276 arg2Of PCR,performed
R4553 T5276 T5275 arg2Of performed,was
R4554 T5277 T5276 arg3Of using,performed
R4555 T5280 T5279 arg1Of Omnigene,Hybaid
R4556 T5282 T5277 arg2Of cycler,using
R4557 T5282 T5278 arg1Of cycler,a
R4558 T5282 T5280 arg1Of cycler,Omnigene
R4559 T5282 T5281 arg1Of cycler,thermal
R4560 T5282 T5283 arg1Of cycler,(
R4561 T5282 T5290 arg1Of cycler,with
R4562 T5284 T5285 arg1Of Hybaid,","
R4563 T5285 T5283 arg2Of ",",(
R4564 T5285 T5287 arg1Of ",",","
R4565 T5286 T5285 arg2Of Ashford,","
R4566 T5288 T5287 arg2Of UK,","
R4567 T5289 T5283 arg3Of ),(
R4568 T5292 T5290 arg2Of parameters,with
R4569 T5292 T5291 arg1Of parameters,cycling
R4570 T5292 T5293 arg1Of parameters,of
R4571 T5292 T5301 arg1Of parameters,at
R4572 T5294 T5295 arg1Of 72°C,for
R4573 T5294 T5298 arg1Of 72°C,","
R4574 T5297 T5295 arg2Of min,for
R4575 T5297 T5296 arg1Of min,10
R4576 T5298 T5293 arg2Of ",",of
R4577 T5300 T5298 arg2Of cycles,","
R4578 T5300 T5299 arg1Of cycles,35
R4579 T5302 T5301 arg2Of 94°C,at
R4580 T5302 T5303 arg1Of 94°C,for
R4581 T5305 T5304 arg1Of s,45
R4582 T5305 T5306 arg1Of s,","
R4583 T5306 T5311 arg1Of ",",","
R4584 T5307 T5306 arg2Of 52°C,","
R4585 T5307 T5308 arg1Of 52°C,for
R4586 T5310 T5308 arg2Of s,for
R4587 T5310 T5309 arg1Of s,45
R4588 T5311 T5303 arg2Of ",",for
R4589 T5311 T5313 arg1Of ",",for
R4590 T5312 T5311 arg2Of 72°C,","
R4591 T5315 T5313 arg2Of s,for
R4592 T5315 T5314 arg1Of s,45
R4724 T5499 T5500 arg1Of GR-GATA-3,In
R4725 T5499 T5504 arg1Of GR-GATA-3,for
R4726 T5503 T5500 arg2Of Assay,In
R4727 T5503 T5501 arg1Of Assay,Vitro
R4728 T5503 T5502 arg1Of Assay,Competition
R4729 T5505 T5504 arg2Of Importin-α,for
R4730 T5505 T5506 arg1Of Importin-α,(
R4731 T5508 T5506 arg2Of ELISA,(
R4732 T5508 T5507 arg1Of ELISA,Far-Western
R4733 T5509 T5506 arg3Of ),(
R4734 T5513 T5510 arg2Of anti-importin-α,Immunoprecipitated
R4735 T5513 T5511 arg1Of anti-importin-α,importin-α
R4736 T5513 T5512 arg2Of anti-importin-α,(
R4737 T5513 T5514 arg1Of anti-importin-α,","
R4738 T5513 T5519 arg1Of anti-importin-α,from
R4739 T5513 T5522 arg1Of anti-importin-α,was
R4740 T5513 T5523 arg2Of anti-importin-α,separated
R4741 T5513 T5527 arg2Of anti-importin-α,purified
R4742 T5517 T5514 arg2Of Biotechnology,","
R4743 T5517 T5515 arg1Of Biotechnology,Santa
R4744 T5517 T5516 arg1Of Biotechnology,Cruz
R4745 T5518 T5512 arg3Of ),(
R4746 T5521 T5519 arg2Of cells,from
R4747 T5521 T5520 arg1Of cells,HuT-78
R4748 T5523 T5526 arg1Of separated,and
R4749 T5525 T5523 arg1Of SDS-PAGE,separated
R4750 T5525 T5524 arg2Of SDS-PAGE,by
R4751 T5526 T5522 arg2Of and,was
R4752 T5527 T5526 arg2Of purified,and
R4753 T5527 T5528 arg1Of purified,from
R4754 T5527 T5534 arg1Of purified,[
R4755 T5531 T5528 arg2Of gel,from
R4756 T5531 T5529 arg1Of gel,the
R4757 T5531 T5530 arg2Of gel,excised
R4758 T5533 T5527 arg1Of electroelution,purified
R4759 T5533 T5532 arg2Of electroelution,by
R4760 T5535 T5534 arg2Of 36,[
R4761 T5536 T5534 arg3Of ],[
R4762 T5539 T5540 arg1Of GATA-3,was
R4763 T5539 T5541 arg2Of GATA-3,isolated
R4764 T5541 T5537 arg1Of isolated,Similarly
R4765 T5541 T5538 arg1Of isolated,","
R4766 T5541 T5540 arg2Of isolated,was
R4767 T5541 T5542 arg1Of isolated,from
R4768 T5541 T5554 arg1Of isolated,and
R4769 T5545 T5543 arg1Of cells,nonstimulated
R4770 T5545 T5544 arg1Of cells,HuT-78
R4771 T5545 T5546 arg1Of cells,or
R4772 T5546 T5542 arg2Of or,from
R4773 T5547 T5546 arg2Of cells,or
R4774 T5547 T5548 arg2Of cells,stimulated
R4775 T5548 T5549 arg1Of stimulated,for
R4776 T5551 T5549 arg2Of min,for
R4777 T5551 T5550 arg1Of min,30
R4778 T5551 T5552 arg1Of min,with
R4779 T5553 T5552 arg2Of anti-CD3/CD28,with
R4780 T5555 T5556 arg1Of GR,was
R4781 T5555 T5557 arg2Of GR,isolated
R4782 T5557 T5554 arg2Of isolated,and
R4783 T5557 T5556 arg2Of isolated,was
R4784 T5557 T5558 arg1Of isolated,from
R4785 T5559 T5560 arg1Of FP,(
R4786 T5562 T5560 arg2Of M,(
R4787 T5562 T5561 arg1Of M,10−8
R4788 T5562 T5563 arg1Of M,","
R4789 T5565 T5563 arg2Of min,","
R4790 T5565 T5564 arg1Of min,30
R4791 T5566 T5560 arg3Of ),(
R4792 T5569 T5558 arg2Of cells,from
R4793 T5569 T5559 arg1Of cells,FP
R4794 T5569 T5567 arg1Of cells,stimulated
R4795 T5569 T5568 arg1Of cells,HuT-78
R4796 T5571 T5570 arg1Of proteins,These
R4797 T5571 T5572 arg1Of proteins,were
R4798 T5571 T5574 arg2Of proteins,refolded
R4799 T5574 T5572 arg2Of refolded,were
R4800 T5574 T5573 arg1Of refolded,subsequently
R4801 T5574 T5575 arg1Of refolded,in
R4802 T5577 T5575 arg2Of solution,in
R4803 T5577 T5576 arg1Of solution,glycine
R4804 T5579 T5578 arg1Of µl,100
R4805 T5579 T5580 arg1Of µl,of
R4806 T5579 T5583 arg1Of µl,(
R4807 T5579 T5589 arg1Of µl,was
R4808 T5579 T5590 arg2Of µl,added
R4809 T5582 T5580 arg2Of solution,of
R4810 T5582 T5581 arg1Of solution,importin-α
R4811 T5585 T5583 arg2Of ng/ml,(
R4812 T5585 T5584 arg1Of ng/ml,100
R4813 T5585 T5586 arg1Of ng/ml,in
R4814 T5587 T5586 arg2Of TBS,in
R4815 T5588 T5583 arg3Of ),(
R4816 T5590 T5589 arg2Of added,was
R4817 T5590 T5591 arg1Of added,to
R4818 T5593 T5591 arg2Of plates,to
R4819 T5593 T5592 arg1Of plates,96-well
R4820 T5593 T5594 arg2Of plates,coated
R4821 T5594 T5595 arg1Of coated,with
R4822 T5598 T5595 arg2Of antibody,with
R4823 T5598 T5596 arg1Of antibody,goat
R4824 T5598 T5597 arg1Of antibody,anti-importin-α
R4825 T5602 T5599 arg2Of incubation,After
R4826 T5602 T5600 arg1Of incubation,1
R4827 T5602 T5601 arg1Of incubation,h
R4828 T5604 T5605 arg1Of GATA-3,(
R4829 T5604 T5611 arg1Of GATA-3,from
R4830 T5604 T5616 arg1Of GATA-3,were
R4831 T5604 T5617 arg2Of GATA-3,added
R4832 T5607 T5605 arg2Of ng/ml,(
R4833 T5607 T5606 arg1Of ng/ml,100
R4834 T5607 T5608 arg1Of ng/ml,in
R4835 T5609 T5608 arg2Of TBS,in
R4836 T5610 T5605 arg3Of ),(
R4837 T5615 T5611 arg2Of cells,from
R4838 T5615 T5612 arg2Of cells,stimulated
R4839 T5615 T5613 arg1Of cells,or
R4840 T5615 T5614 arg1Of cells,unstimulated
R4841 T5617 T5599 arg1Of added,After
R4842 T5617 T5603 arg1Of added,","
R4843 T5617 T5616 arg2Of added,were
R4844 T5617 T5618 arg1Of added,to
R4845 T5621 T5618 arg2Of wells,to
R4846 T5621 T5619 arg1Of wells,the
R4847 T5621 T5620 arg1Of wells,importin-α-coated
R4848 T5622 T5623 arg1Of GR,(
R4849 T5622 T5631 arg1Of GR,from
R4850 T5622 T5636 arg1Of GR,were
R4851 T5622 T5637 arg2Of GR,added
R4852 T5624 T5625 arg1Of 10,or
R4853 T5626 T5625 arg2Of 100,or
R4854 T5627 T5623 arg2Of ng/ml,(
R4855 T5627 T5624 arg1Of ng/ml,10
R4856 T5627 T5626 arg1Of ng/ml,100
R4857 T5627 T5628 arg1Of ng/ml,in
R4858 T5629 T5628 arg2Of TBS,in
R4859 T5630 T5623 arg3Of ),(
R4860 T5635 T5631 arg2Of cells,from
R4861 T5635 T5632 arg2Of cells,stimulated
R4862 T5635 T5633 arg1Of cells,or
R4863 T5635 T5634 arg1Of cells,unstimulated
R4864 T5637 T5636 arg2Of added,were
R4865 T5637 T5638 arg1Of added,to
R4866 T5640 T5638 arg2Of wells,to
R4867 T5640 T5639 arg1Of wells,some
R4868 T5644 T5645 arg1Of 1,h
R4869 T5646 T5641 arg2Of incubation,After
R4870 T5646 T5642 arg1Of incubation,a
R4871 T5646 T5643 arg1Of incubation,further
R4872 T5646 T5644 arg1Of incubation,1
R4873 T5649 T5648 arg1Of plate,the
R4874 T5649 T5650 arg1Of plate,was
R4875 T5649 T5651 arg2Of plate,washed
R4876 T5649 T5653 arg2Of plate,incubated
R4877 T5649 T5677 arg2Of plate,incubated
R4878 T5651 T5652 arg1Of washed,and
R4879 T5652 T5654 arg1Of and,with
R4880 T5652 T5675 arg1Of and,and
R4881 T5653 T5652 arg2Of incubated,and
R4882 T5656 T5654 arg2Of antibodies,with
R4883 T5656 T5655 arg1Of antibodies,primary
R4884 T5656 T5657 arg1Of antibodies,(
R4885 T5656 T5671 arg1Of antibodies,for
R4886 T5659 T5657 arg2Of mixture,(
R4887 T5659 T5658 arg1Of mixture,a
R4888 T5659 T5660 arg1Of mixture,of
R4889 T5662 T5661 arg1Of anti-GATA-3,rabbit
R4890 T5662 T5663 arg1Of anti-GATA-3,and
R4891 T5663 T5660 arg2Of and,of
R4892 T5663 T5666 arg1Of and,","
R4893 T5665 T5663 arg2Of anti-GR,and
R4894 T5665 T5664 arg1Of anti-GR,mouse
R4895 T5669 T5666 arg2Of Biotechnology,","
R4896 T5669 T5667 arg1Of Biotechnology,Santa
R4897 T5669 T5668 arg1Of Biotechnology,Cruz
R4898 T5670 T5657 arg3Of ),(
R4899 T5673 T5671 arg2Of h,for
R4900 T5673 T5672 arg1Of h,1.5
R4901 T5675 T5641 arg1Of and,After
R4902 T5675 T5647 arg1Of and,","
R4903 T5675 T5650 arg2Of and,was
R4904 T5675 T5674 arg1Of and,","
R4905 T5677 T5675 arg2Of incubated,and
R4906 T5677 T5676 arg1Of incubated,then
R4907 T5677 T5678 arg1Of incubated,with
R4908 T5680 T5678 arg2Of antibodies,with
R4909 T5680 T5679 arg1Of antibodies,secondary
R4910 T5684 T5681 arg1Of IgG,FITC
R4911 T5684 T5682 arg1Of IgG,swine
R4912 T5684 T5683 arg1Of IgG,anti-rabbit
R4913 T5684 T5685 arg1Of IgG,(
R4914 T5684 T5692 arg1Of IgG,was
R4915 T5684 T5693 arg2Of IgG,used
R4916 T5686 T5685 arg2Of Dako,(
R4917 T5686 T5687 arg1Of Dako,","
R4918 T5688 T5687 arg2Of Cambridge,","
R4919 T5688 T5689 arg1Of Cambridge,","
R4920 T5690 T5689 arg2Of UK,","
R4921 T5691 T5685 arg3Of ),(
R4922 T5693 T5692 arg2Of used,was
R4923 T5693 T5694 arg1Of used,for
R4924 T5693 T5700 arg1Of used,and
R4925 T5696 T5694 arg2Of detection,for
R4926 T5696 T5695 arg1Of detection,the
R4927 T5696 T5697 arg1Of detection,of
R4928 T5698 T5697 arg2Of GATA-3,of
R4929 T5700 T5699 arg1Of and,","
R4930 T5704 T5701 arg1Of IgG,rhodamine
R4931 T5704 T5702 arg1Of IgG,donkey
R4932 T5704 T5703 arg1Of IgG,anti-mouse
R4933 T5704 T5705 arg1Of IgG,(
R4934 T5704 T5714 arg1Of IgG,was
R4935 T5704 T5715 arg2Of IgG,used
R4936 T5706 T5707 arg1Of Novus,","
R4937 T5707 T5709 arg1Of ",",","
R4938 T5708 T5707 arg2Of Littleton,","
R4939 T5709 T5705 arg2Of ",",(
R4940 T5709 T5711 arg1Of ",",","
R4941 T5709 T5712 arg1Of ",",USA
R4942 T5710 T5709 arg2Of Colorado,","
R4943 T5713 T5705 arg3Of ),(
R4944 T5715 T5700 arg2Of used,and
R4945 T5715 T5714 arg2Of used,was
R4946 T5715 T5716 arg1Of used,for
R4947 T5718 T5716 arg2Of detection,for
R4948 T5718 T5717 arg1Of detection,the
R4949 T5718 T5719 arg1Of detection,of
R4950 T5720 T5719 arg2Of GR,of
R4951 T5721 T5722 arg1Of FITC,and
R4952 T5723 T5722 arg2Of rhodamine,and
R4953 T5724 T5721 arg1Of levels,FITC
R4954 T5724 T5723 arg1Of levels,rhodamine
R4955 T5724 T5725 arg1Of levels,were
R4956 T5724 T5726 arg2Of levels,measured
R4957 T5726 T5725 arg2Of measured,were
R4958 T5726 T5727 arg1Of measured,with
R4959 T5726 T5732 arg1Of measured,(
R4960 T5731 T5727 arg2Of reader,with
R4961 T5731 T5728 arg1Of reader,a
R4962 T5731 T5729 arg1Of reader,fluorescent
R4963 T5731 T5730 arg1Of reader,micro-plate
R4964 T5733 T5732 arg2Of Bioline,(
R4965 T5733 T5734 arg1Of Bioline,","
R4966 T5735 T5734 arg2Of London,","
R4967 T5735 T5736 arg1Of London,","
R4968 T5737 T5736 arg2Of UK,","
R4969 T5738 T5732 arg3Of ),(
R5227 T6081 T6080 arg1Of Activation,NF-κB
R5228 T6084 T6082 arg1Of activity,NF-κB
R5229 T6084 T6083 arg1Of activity,binding
R5230 T6084 T6085 arg1Of activity,in
R5231 T6084 T6088 arg1Of activity,was
R5232 T6084 T6089 arg2Of activity,determined
R5233 T6087 T6085 arg2Of extracts,in
R5234 T6087 T6086 arg1Of extracts,nuclear
R5235 T6089 T6088 arg2Of determined,was
R5236 T6090 T6089 arg3Of using,determined
R5237 T6093 T6090 arg2Of kit,using
R5238 T6093 T6091 arg1Of kit,an
R5239 T6093 T6092 arg1Of kit,ELISA-based
R5240 T6093 T6094 arg1Of kit,(
R5241 T6096 T6095 arg1Of p65,Trans-AM
R5242 T6096 T6097 arg1Of p65,","
R5243 T6096 T6100 arg1Of p65,","
R5244 T6099 T6097 arg2Of Motif,","
R5245 T6099 T6098 arg1Of Motif,Active
R5246 T6100 T6094 arg2Of ",",(
R5247 T6101 T6100 arg2Of Rixensart,","
R5248 T6101 T6102 arg1Of Rixensart,","
R5249 T6103 T6102 arg2Of Belgium,","
R5250 T6104 T6094 arg3Of ),(
R5251 T6106 T6105 arg2Of brief,In
R5252 T6109 T6108 arg1Of µg,5
R5253 T6109 T6110 arg1Of µg,of
R5254 T6109 T6113 arg1Of µg,were
R5255 T6109 T6114 arg2Of µg,incubated
R5256 T6112 T6110 arg2Of extracts,of
R5257 T6112 T6111 arg1Of extracts,nuclear
R5258 T6114 T6105 arg1Of incubated,In
R5259 T6114 T6107 arg1Of incubated,","
R5260 T6114 T6113 arg2Of incubated,were
R5261 T6114 T6115 arg1Of incubated,with
R5262 T6117 T6115 arg2Of plate,with
R5263 T6117 T6116 arg1Of plate,a
R5264 T6117 T6118 arg2Of plate,coated
R5265 T6118 T6119 arg1Of coated,with
R5266 T6123 T6119 arg2Of oligonucleotide,with
R5267 T6123 T6120 arg1Of oligonucleotide,an
R5268 T6123 T6121 arg1Of oligonucleotide,NF-κB
R5269 T6123 T6122 arg1Of oligonucleotide,consensus
R5270 T6124 T6125 arg1Of Plates,were
R5271 T6124 T6126 arg2Of Plates,washed
R5272 T6126 T6125 arg2Of washed,were
R5273 T6126 T6127 arg1Of washed,before
R5274 T6128 T6127 arg2Of addition,before
R5275 T6128 T6129 arg1Of addition,of
R5276 T6132 T6129 arg2Of antibody,of
R5277 T6132 T6130 arg1Of antibody,an
R5278 T6132 T6131 arg1Of antibody,anti-p65
R5279 T6134 T6133 arg1Of binding,Antibody
R5280 T6134 T6135 arg1Of binding,was
R5281 T6134 T6136 arg2Of binding,detected
R5282 T6134 T6143 arg2Of binding,developed
R5283 T6136 T6137 arg1Of detected,with
R5284 T6136 T6142 arg1Of detected,and
R5285 T6141 T6137 arg2Of antibody,with
R5286 T6141 T6138 arg1Of antibody,a
R5287 T6141 T6139 arg1Of antibody,secondary
R5288 T6141 T6140 arg1Of antibody,HRP-conjugated
R5289 T6142 T6135 arg2Of and,was
R5290 T6143 T6142 arg2Of developed,and
R5291 T6143 T6144 arg1Of developed,with
R5292 T6146 T6144 arg2Of substrate,with
R5293 T6146 T6145 arg1Of substrate,TMB
R5294 T6148 T6147 arg1Of intensity,The
R5295 T6148 T6149 arg1Of intensity,of
R5296 T6148 T6152 arg1Of intensity,was
R5297 T6148 T6153 arg2Of intensity,measured
R5298 T6151 T6149 arg2Of reaction,of
R5299 T6151 T6150 arg1Of reaction,the
R5300 T6153 T6152 arg2Of measured,was
R5301 T6153 T6154 arg1Of measured,at
R5302 T6156 T6154 arg2Of nm,at
R5303 T6156 T6155 arg1Of nm,450
R5389 T6284 T6283 arg1Of Staining,Immunofluorescence
R5390 T6286 T6285 arg1Of staining,Immunofluorescence
R5391 T6286 T6287 arg1Of staining,was
R5392 T6286 T6288 arg2Of staining,performed
R5393 T6288 T6287 arg2Of performed,was
R5394 T6288 T6289 arg1Of performed,as
R5395 T6291 T6290 arg1Of described,previously
R5396 T6293 T6289 arg2Of 34,as
R5397 T6293 T6291 arg1Of 34,described
R5398 T6293 T6292 arg2Of 34,[
R5399 T6294 T6292 arg3Of ],[
R5400 T6296 T6295 arg1Of staining,All
R5401 T6296 T6297 arg1Of staining,was
R5402 T6296 T6298 arg2Of staining,performed
R5403 T6298 T6297 arg2Of performed,was
R5404 T6298 T6299 arg1Of performed,at
R5405 T6298 T6303 arg1Of performed,under
R5406 T6299 T6302 arg1Of at,and
R5407 T6301 T6299 arg2Of temperature,at
R5408 T6301 T6300 arg1Of temperature,room
R5409 T6303 T6302 arg2Of under,and
R5410 T6304 T6303 arg2Of humidification,under
R5411 T6305 T6306 arg1Of Cells,were
R5412 T6305 T6307 arg2Of Cells,collected
R5413 T6307 T6306 arg2Of collected,were
R5414 T6307 T6308 arg1Of collected,and
R5415 T6308 T6314 arg1Of and,(
R5416 T6309 T6310 arg2Of cytospins,prepared
R5417 T6310 T6308 arg2Of prepared,and
R5418 T6310 T6311 arg1Of prepared,in
R5419 T6313 T6311 arg2Of cytocentrifuge,in
R5420 T6313 T6312 arg1Of cytocentrifuge,a
R5421 T6316 T6315 arg1Of II,Shandon
R5422 T6316 T6317 arg1Of II,","
R5423 T6317 T6319 arg1Of ",",","
R5424 T6318 T6317 arg2Of Shandon,","
R5425 T6319 T6314 arg2Of ",",(
R5426 T6319 T6321 arg1Of ",",","
R5427 T6320 T6319 arg2Of Runcorn,","
R5428 T6322 T6321 arg2Of UK,","
R5429 T6323 T6314 arg3Of ),(
R5430 T6324 T6325 arg1Of Cells,were
R5431 T6324 T6326 arg2Of Cells,permeabilized
R5432 T6324 T6328 arg2Of Cells,blocked
R5433 T6324 T6332 arg2Of Cells,incubated
R5434 T6326 T6327 arg1Of permeabilized,","
R5435 T6327 T6330 arg1Of ",",and
R5436 T6328 T6327 arg2Of blocked,","
R5437 T6330 T6325 arg2Of and,were
R5438 T6330 T6329 arg1Of and,","
R5439 T6332 T6330 arg2Of incubated,and
R5440 T6332 T6331 arg1Of incubated,then
R5441 T6332 T6333 arg1Of incubated,with
R5442 T6334 T6335 arg1Of GR,(
R5443 T6334 T6342 arg1Of GR,or
R5444 T6340 T6335 arg2Of Biotechnology,(
R5445 T6340 T6336 arg1Of Biotechnology,1∶50
R5446 T6340 T6337 arg1Of Biotechnology,E-20
R5447 T6340 T6338 arg1Of Biotechnology,Santa
R5448 T6340 T6339 arg1Of Biotechnology,Cruz
R5449 T6341 T6335 arg3Of ),(
R5450 T6342 T6333 arg2Of or,with
R5451 T6343 T6344 arg1Of anti-GATA-3,(
R5452 T6345 T6344 arg2Of H-48,(
R5453 T6345 T6346 arg1Of H-48,;
R5454 T6349 T6346 arg2Of Biotechnology,;
R5455 T6349 T6347 arg1Of Biotechnology,Santa
R5456 T6349 T6348 arg1Of Biotechnology,Cruz
R5457 T6350 T6344 arg3Of ),(
R5458 T6351 T6342 arg2Of antibody,or
R5459 T6351 T6343 arg1Of antibody,anti-GATA-3
R5460 T6351 T6352 arg1Of antibody,for
R5461 T6354 T6352 arg2Of h,for
R5462 T6354 T6353 arg1Of h,1
R5463 T6354 T6355 arg1Of h,at
R5464 T6357 T6355 arg2Of temperature,at
R5465 T6357 T6356 arg1Of temperature,room
R5466 T6360 T6358 arg2Of washes,After
R5467 T6360 T6359 arg1Of washes,three
R5468 T6360 T6361 arg1Of washes,in
R5469 T6363 T6361 arg2Of saline,in
R5470 T6363 T6362 arg1Of saline,phosphate-buffered
R5471 T6363 T6364 arg1Of saline,(
R5472 T6365 T6364 arg2Of PBS,(
R5473 T6366 T6364 arg3Of ),(
R5474 T6368 T6369 arg1Of cells,were
R5475 T6368 T6370 arg2Of cells,incubated
R5476 T6370 T6358 arg1Of incubated,After
R5477 T6370 T6367 arg1Of incubated,","
R5478 T6370 T6369 arg2Of incubated,were
R5479 T6370 T6371 arg1Of incubated,with
R5480 T6372 T6371 arg2Of tetrarhodamine,with
R5481 T6372 T6373 arg2Of tetrarhodamine,isothiocyanate–conjugated
R5482 T6376 T6373 arg3Of antibody,isothiocyanate–conjugated
R5483 T6376 T6374 arg1Of antibody,goat
R5484 T6376 T6375 arg1Of antibody,anti-rabbit
R5485 T6376 T6377 arg1Of antibody,(
R5486 T6378 T6377 arg2Of Dako,(
R5487 T6379 T6377 arg3Of ),(
R5488 T6380 T6381 arg1Of Cytospins,were
R5489 T6380 T6382 arg2Of Cytospins,counterstained
R5490 T6380 T6401 arg1Of Cytospins,mounted
R5491 T6382 T6381 arg2Of counterstained,were
R5492 T6382 T6383 arg1Of counterstained,with
R5493 T6382 T6400 arg1Of counterstained,and
R5494 T6384 T6383 arg2Of 4′,with
R5495 T6384 T6385 arg1Of 4′,","
R5496 T6384 T6391 arg1Of 4′,","
R5497 T6387 T6385 arg2Of dihydrochloride,","
R5498 T6387 T6386 arg1Of dihydrochloride,6-diamidino-2-phenylindole
R5499 T6387 T6388 arg1Of dihydrochloride,(
R5500 T6389 T6388 arg2Of DAPI,(
R5501 T6390 T6388 arg3Of ),(
R5502 T6398 T6391 arg2Of stain,","
R5503 T6398 T6392 arg1Of stain,a
R5504 T6398 T6393 arg1Of stain,fluorescent
R5505 T6398 T6394 arg1Of stain,blue
R5506 T6398 T6395 arg1Of stain,nuclear
R5507 T6398 T6396 arg1Of stain,indole
R5508 T6398 T6397 arg1Of stain,chromatin
R5509 T6400 T6399 arg1Of and,","
R5510 T6401 T6400 arg2Of mounted,and
R5511 T6401 T6402 arg1Of mounted,in
R5512 T6401 T6404 arg1Of mounted,:
R5513 T6403 T6402 arg2Of PBS,in
R5514 T6405 T6401 arg2Of glycerol,mounted
R5515 T6405 T6406 arg1Of glycerol,(
R5516 T6407 T6406 arg2Of 50∶50,(
R5517 T6408 T6406 arg3Of ),(
R5518 T6411 T6409 arg1Of signal,The
R5519 T6411 T6410 arg1Of signal,immunopositive
R5520 T6411 T6412 arg1Of signal,was
R5521 T6411 T6413 arg2Of signal,characterised
R5522 T6411 T6414 arg1Of signal,using
R5523 T6413 T6412 arg2Of characterised,was
R5524 T6414 T6413 arg3Of using,characterised
R5525 T6414 T6419 arg1Of using,on
R5526 T6418 T6414 arg2Of microscopy,using
R5527 T6418 T6415 arg1Of microscopy,laser
R5528 T6418 T6416 arg1Of microscopy,scanning
R5529 T6418 T6417 arg1Of microscopy,confocal
R5530 T6423 T6421 arg1Of NT/SP,Leica
R5531 T6423 T6422 arg1Of NT/SP,TCS
R5532 T6426 T6419 arg2Of cytometer,on
R5533 T6426 T6420 arg1Of cytometer,a
R5534 T6426 T6423 arg1Of cytometer,NT/SP
R5535 T6426 T6424 arg1Of cytometer,interactive
R5536 T6426 T6425 arg1Of cytometer,laser
R5537 T6426 T6427 arg2Of cytometer,equipped
R5538 T6427 T6428 arg1Of equipped,with
R5539 T6430 T6428 arg2Of optics,with
R5540 T6430 T6429 arg1Of optics,confocal
R5541 T6430 T6431 arg1Of optics,(
R5542 T6433 T6431 arg2Of Microsystems,(
R5543 T6433 T6432 arg1Of Microsystems,Leica
R5544 T6433 T6434 arg1Of Microsystems,","
R5545 T6435 T6434 arg2Of Wetzlar,","
R5546 T6435 T6436 arg1Of Wetzlar,","
R5547 T6437 T6436 arg2Of Germany,","
R5548 T6438 T6431 arg3Of ),(
R5549 T6440 T6439 arg1Of determine,To
R5550 T6442 T6440 arg2Of specificity,determine
R5551 T6442 T6441 arg1Of specificity,the
R5552 T6442 T6443 arg1Of specificity,of
R5553 T6445 T6443 arg2Of antibodies,of
R5554 T6445 T6444 arg1Of antibodies,the
R5555 T6449 T6447 arg1Of immunoglobulin,rabbit
R5556 T6449 T6448 arg1Of immunoglobulin,serum
R5557 T6449 T6450 arg1Of immunoglobulin,(
R5558 T6449 T6453 arg1Of immunoglobulin,and
R5559 T6451 T6450 arg2Of Dako,(
R5560 T6452 T6450 arg3Of ),(
R5561 T6453 T6456 arg1Of and,without
R5562 T6453 T6459 arg1Of and,were
R5563 T6453 T6460 arg2Of and,used
R5564 T6455 T6453 arg2Of antibodies,and
R5565 T6455 T6454 arg1Of antibodies,secondary
R5566 T6458 T6456 arg2Of primary,without
R5567 T6458 T6457 arg1Of primary,the
R5568 T6460 T6439 modOf used,To
R5569 T6460 T6446 arg1Of used,","
R5570 T6460 T6459 arg2Of used,were
R5571 T6460 T6461 arg1Of used,as
R5572 T6462 T6461 arg2Of controls,as
R5573 T6464 T6463 arg1Of stained,Positively
R5574 T6465 T6464 arg1Of nuclei,stained
R5575 T6465 T6466 arg1Of nuclei,and
R5576 T6466 T6469 arg1Of and,were
R5577 T6466 T6470 arg2Of and,counted
R5578 T6468 T6466 arg2Of cells,and
R5579 T6468 T6467 arg1Of cells,total
R5580 T6470 T6469 arg2Of counted,were
R5581 T6470 T6471 arg1Of counted,(
R5582 T6470 T6474 arg1Of counted,on
R5583 T6472 T6471 arg2Of 500,(
R5584 T6473 T6471 arg3Of ),(
R5585 T6476 T6474 arg2Of slide,on
R5586 T6476 T6475 arg1Of slide,each
R5587 T6476 T6477 arg1Of slide,with
R5588 T6479 T6477 arg2Of observer,with
R5589 T6479 T6478 arg1Of observer,the
R5590 T6479 T6480 arg2Of observer,blinded
R5591 T6480 T6481 arg1Of blinded,to
R5592 T6483 T6481 arg2Of treatment,to
R5593 T6483 T6482 arg1Of treatment,the
R5815 T6780 T6779 arg1Of Construct,GATA-3-GFP
R5816 T6785 T6784 arg2Of BC003070,(
R5817 T6786 T6784 arg3Of ),(
R5818 T6788 T6781 arg1Of cDNA,The
R5819 T6788 T6782 arg1Of cDNA,GATA-3
R5820 T6788 T6783 arg1Of cDNA,clone
R5821 T6788 T6784 arg1Of cDNA,(
R5822 T6788 T6787 arg1Of cDNA,complete
R5823 T6788 T6789 arg1Of cDNA,was
R5824 T6788 T6790 arg2Of cDNA,obtained
R5825 T6790 T6789 arg2Of obtained,was
R5826 T6790 T6791 arg1Of obtained,from
R5827 T6790 T6795 arg1Of obtained,as
R5828 T6794 T6791 arg2Of Technologies,from
R5829 T6794 T6792 arg1Of Technologies,Invitrogen
R5830 T6794 T6793 arg1Of Technologies,Life
R5831 T6799 T6795 arg2Of insert,as
R5832 T6799 T6796 arg1Of insert,a
R5833 T6799 T6797 arg1Of insert,5′-
R5834 T6799 T6798 arg1Of insert,EcoRI/3′-XhoI
R5835 T6799 T6800 arg1Of insert,of
R5836 T6799 T6802 arg1Of insert,in
R5837 T6801 T6800 arg2Of GATA-3,of
R5838 T6805 T6802 arg2Of vector,in
R5839 T6805 T6803 arg1Of vector,the
R5840 T6805 T6804 arg1Of vector,pOTB7
R5841 T6806 T6807 arg1Of GATA-3,was
R5842 T6806 T6808 arg2Of GATA-3,excised
R5843 T6808 T6807 arg2Of excised,was
R5844 T6808 T6809 arg1Of excised,from
R5845 T6810 T6809 arg2Of pOTB7,from
R5846 T6813 T6811 arg1Of digestion,using
R5847 T6813 T6812 arg1Of digestion,XhoI
R5848 T6813 T6814 arg1Of digestion,and
R5849 T6814 T6823 arg1Of and,was
R5850 T6814 T6824 arg2Of and,digested
R5851 T6815 T6814 arg2Of pEGFP-C2,and
R5852 T6815 T6816 arg1Of pEGFP-C2,(
R5853 T6817 T6818 arg1Of Clontech,","
R5854 T6818 T6820 arg1Of ",",","
R5855 T6819 T6818 arg2Of Saint-Germain-en-Laye,","
R5856 T6820 T6816 arg2Of ",",(
R5857 T6821 T6820 arg2Of France,","
R5858 T6822 T6816 arg3Of ),(
R5859 T6824 T6807 modOf digested,was
R5860 T6824 T6823 arg2Of digested,was
R5861 T6824 T6825 arg1Of digested,with
R5862 T6826 T6825 arg2Of BamHI,with
R5863 T6827 T6828 arg1Of DNA,was
R5864 T6827 T6829 arg2Of DNA,recovered
R5865 T6829 T6828 arg2Of recovered,was
R5866 T6832 T6831 arg1Of extraction,phenol
R5867 T6832 T6833 arg1Of extraction,and
R5868 T6833 T6829 arg1Of and,recovered
R5869 T6833 T6830 arg2Of and,by
R5870 T6835 T6833 arg2Of precipitation,and
R5871 T6835 T6834 arg1Of precipitation,ethanol
R5872 T6837 T6836 arg1Of and,","
R5873 T6841 T6839 arg1Of fragment,the
R5874 T6841 T6840 arg1Of fragment,GATA-3
R5875 T6841 T6842 arg1Of fragment,and
R5876 T6842 T6838 arg1Of and,both
R5877 T6845 T6842 arg2Of vector,and
R5878 T6845 T6843 arg1Of vector,the
R5879 T6845 T6844 arg1Of vector,pEGFP-C2
R5880 T6845 T6846 arg1Of vector,blunt-ended
R5881 T6848 T6847 arg2Of incubation,by
R5882 T6850 T6849 arg2Of Klenow,with
R5883 T6852 T6851 arg2Of Bioline,(
R5884 T6852 T6853 arg1Of Bioline,Bio-27029
R5885 T6854 T6851 arg3Of ),(
R5886 T6857 T6855 arg2Of min,for
R5887 T6857 T6856 arg1Of min,30
R5888 T6860 T6859 arg1Of Klenow,37°C.
R5889 T6860 T6861 arg1Of Klenow,was
R5890 T6860 T6862 arg2Of Klenow,inactivated
R5891 T6862 T6861 arg2Of inactivated,was
R5892 T6862 T6863 arg1Of inactivated,by
R5893 T6864 T6863 arg2Of incubation,by
R5894 T6864 T6865 arg1Of incubation,for
R5895 T6867 T6865 arg2Of min,for
R5896 T6867 T6866 arg1Of min,10
R5897 T6867 T6868 arg1Of min,at
R5898 T6870 T6868 arg2Of DNA,at
R5899 T6870 T6869 arg1Of DNA,75°C.
R5900 T6872 T6871 arg2Of recovered,was
R5901 T6875 T6874 arg1Of extraction,phenol
R5902 T6875 T6876 arg1Of extraction,and
R5903 T6876 T6872 arg1Of and,recovered
R5904 T6876 T6873 arg2Of and,by
R5905 T6878 T6876 arg2Of precipitation,and
R5906 T6878 T6877 arg1Of precipitation,ethanol
R5907 T6880 T6879 arg1Of and,","
R5908 T6885 T6882 arg1Of fragment,the
R5909 T6885 T6883 arg1Of fragment,blunt-ended
R5910 T6885 T6884 arg1Of fragment,GATA-3
R5911 T6885 T6886 arg1Of fragment,and
R5912 T6886 T6881 arg1Of and,both
R5913 T6886 T6890 arg1Of and,were
R5914 T6886 T6892 arg2Of and,digested
R5915 T6889 T6886 arg2Of vector,and
R5916 T6889 T6887 arg1Of vector,the
R5917 T6889 T6888 arg1Of vector,GFP
R5918 T6892 T6880 arg2Of digested,and
R5919 T6892 T6890 arg2Of digested,were
R5920 T6892 T6891 arg1Of digested,subsequently
R5921 T6892 T6893 arg1Of digested,with
R5922 T6892 T6895 arg1Of digested,before
R5923 T6894 T6893 arg2Of EcoRI,with
R5924 T6900 T6896 arg1Of fragment,the
R5925 T6900 T6897 arg1Of fragment,5′-EcoRI/3′–blunt
R5926 T6900 T6898 arg1Of fragment,end
R5927 T6900 T6899 arg1Of fragment,GATA-3
R5928 T6900 T6901 arg1Of fragment,was
R5929 T6900 T6902 arg2Of fragment,inserted
R5930 T6902 T6895 arg2Of inserted,before
R5931 T6902 T6901 arg2Of inserted,was
R5932 T6902 T6903 arg1Of inserted,into
R5933 T6906 T6905 arg1Of ended,5′-EcoRI/3′–blunt
R5934 T6908 T6903 arg2Of vector,into
R5935 T6908 T6904 arg1Of vector,the
R5936 T6908 T6906 arg1Of vector,ended
R5937 T6908 T6907 arg1Of vector,GFP
R5938 T6910 T6909 arg1Of clones,Positive
R5939 T6910 T6911 arg1Of clones,were
R5940 T6910 T6912 arg2Of clones,confirmed
R5941 T6912 T6911 arg2Of confirmed,were
R5942 T6912 T6918 arg1Of confirmed,by
R5943 T6912 T6925 arg1Of confirmed,by
R5944 T6914 T6915 arg1Of digestion,and
R5945 T6916 T6915 arg2Of size,and
R5946 T6917 T6912 arg1Of analysis,confirmed
R5947 T6917 T6913 arg2Of analysis,by
R5948 T6917 T6914 arg1Of analysis,digestion
R5949 T6917 T6916 arg1Of analysis,size
R5950 T6918 T6924 arg1Of by,and
R5951 T6919 T6920 arg1Of 1,%
R5952 T6923 T6918 arg2Of electrophoresis,by
R5953 T6923 T6919 arg1Of electrophoresis,1
R5954 T6923 T6921 arg1Of electrophoresis,agarose
R5955 T6923 T6922 arg1Of electrophoresis,gel
R5956 T6925 T6924 arg2Of by,and
R5957 T6926 T6925 arg2Of sequencing,by
R6131 T7174 T7173 arg1Of cells,HuT-78
R6132 T7174 T7175 arg1Of cells,were
R6133 T7174 T7176 arg2Of cells,transfected
R6134 T7176 T7175 arg2Of transfected,were
R6135 T7176 T7177 arg1Of transfected,with
R6136 T7179 T7180 arg1Of EP8,or
R6137 T7180 T7177 arg2Of or,with
R6138 T7180 T7178 arg1Of or,either
R6139 T7182 T7180 arg2Of vector,or
R6140 T7182 T7181 arg1Of vector,GFP
R6141 T7184 T7183 arg1Of DNA,only
R6142 T7184 T7185 arg1Of DNA,using
R6143 T7184 T7197 arg1Of DNA,µg
R6144 T7187 T7185 arg2Of R,using
R6145 T7187 T7186 arg1Of R,solution
R6146 T7187 T7188 arg1Of R,","
R6147 T7190 T7188 arg2Of V-001,","
R6148 T7190 T7189 arg1Of V-001,programme
R6149 T7190 T7191 arg1Of V-001,at
R6150 T7193 T7191 arg2Of ratio,at
R6151 T7193 T7192 arg1Of ratio,a
R6152 T7193 T7194 arg1Of ratio,of
R6153 T7196 T7194 arg2Of cells/4,of
R6154 T7196 T7195 arg1Of cells/4,3×106
R6155 T7197 T7175 modOf µg,were
R6156 T7197 T7199 arg1Of µg,for
R6157 T7197 T7202 arg1Of µg,in
R6158 T7197 T7214 arg1Of µg,according
R6159 T7198 T7197 arg2Of DNA,µg
R6160 T7201 T7199 arg2Of h,for
R6161 T7201 T7200 arg1Of h,7–8
R6162 T7204 T7202 arg2Of medium,in
R6163 T7204 T7203 arg1Of medium,complete
R6164 T7204 T7205 arg1Of medium,(
R6165 T7206 T7207 arg1Of 10,%
R6166 T7209 T7205 arg2Of serum,(
R6167 T7209 T7206 arg1Of serum,10
R6168 T7209 T7208 arg1Of serum,bovine
R6169 T7209 T7210 arg1Of serum,in
R6170 T7212 T7210 arg2Of L-glutamine,in
R6171 T7212 T7211 arg1Of L-glutamine,RPMI1640+15
R6172 T7213 T7205 arg3Of ),(
R6173 T7215 T7214 arg2Of to,according
R6174 T7219 T7215 arg2Of protocol,to
R6175 T7219 T7216 arg1Of protocol,the
R6176 T7219 T7217 arg1Of protocol,general
R6177 T7219 T7218 arg1Of protocol,Amaxa
R6178 T7219 T7220 arg1Of protocol,for
R6179 T7221 T7220 arg2Of nucleofection,for
R6180 T7223 T7222 arg1Of medium,The
R6181 T7223 T7224 arg1Of medium,was
R6182 T7223 T7226 arg2Of medium,changed
R6183 T7226 T7224 arg2Of changed,was
R6184 T7226 T7225 arg1Of changed,subsequently
R6185 T7226 T7227 arg1Of changed,to
R6186 T7226 T7231 arg1Of changed,for
R6187 T7228 T7229 arg1Of 1,%
R6188 T7230 T7227 arg2Of RPMI,to
R6189 T7230 T7228 arg1Of RPMI,1
R6190 T7233 T7232 arg1Of h,24
R6191 T7233 T7234 arg1Of h,before
R6192 T7233 T7243 arg1Of h,and
R6193 T7236 T7235 arg2Of cells,transfected
R6194 T7236 T7237 arg1Of cells,were
R6195 T7236 T7238 arg2Of cells,added
R6196 T7238 T7234 arg2Of added,before
R6197 T7238 T7237 arg2Of added,were
R6198 T7238 T7239 arg1Of added,to
R6199 T7242 T7239 arg2Of wells,to
R6200 T7242 T7240 arg1Of wells,anti-CD3/CD28
R6201 T7242 T7241 arg2Of wells,treated
R6202 T7243 T7231 arg2Of and,for
R6203 T7246 T7243 arg2Of videomicroscopy,and
R6204 T7246 T7244 arg1Of videomicroscopy,live
R6205 T7246 T7245 arg1Of videomicroscopy,cell
R6206 T7246 T7247 arg2Of videomicroscopy,performed
R6294 T7358 T7357 arg1Of Microscopy,Time-Lapse
R6295 T7360 T7359 arg1Of cells,HuT-78
R6296 T7360 T7361 arg1Of cells,expressing
R6297 T7360 T7363 arg1Of cells,were
R6298 T7360 T7364 arg2Of cells,maintained
R6299 T7362 T7361 arg2Of GATA-3-GFP,expressing
R6300 T7364 T7363 arg2Of maintained,were
R6301 T7364 T7365 arg1Of maintained,at
R6302 T7364 T7367 arg1Of maintained,in
R6303 T7366 T7365 arg2Of 37°C,at
R6304 T7369 T7367 arg2Of medium,in
R6305 T7369 T7368 arg1Of medium,growth
R6306 T7369 T7370 arg1Of medium,in
R6307 T7369 T7376 arg1Of medium,(
R6308 T7369 T7385 arg2Of medium,combined
R6309 T7375 T7370 arg2Of chamber,in
R6310 T7375 T7371 arg1Of chamber,a
R6311 T7375 T7372 arg1Of chamber,closed
R6312 T7375 T7373 arg1Of chamber,FCS2
R6313 T7375 T7374 arg1Of chamber,perfusion
R6314 T7377 T7378 arg1Of Bioptechs,","
R6315 T7378 T7380 arg1Of ",",","
R6316 T7379 T7378 arg2Of Butler,","
R6317 T7380 T7376 arg2Of ",",(
R6318 T7380 T7382 arg1Of ",",","
R6319 T7380 T7383 arg1Of ",",USA
R6320 T7381 T7380 arg2Of Pennsylvania,","
R6321 T7384 T7376 arg3Of ),(
R6322 T7385 T7386 arg1Of combined,with
R6323 T7385 T7393 arg1Of combined,on
R6324 T7385 T7401 arg1Of combined,(
R6325 T7389 T7386 arg2Of heater,with
R6326 T7389 T7387 arg1Of heater,an
R6327 T7389 T7388 arg1Of heater,objective
R6328 T7389 T7390 arg1Of heater,(
R6329 T7391 T7390 arg2Of Bioptechs,(
R6330 T7392 T7390 arg3Of ),(
R6331 T7395 T7393 arg2Of stage,on
R6332 T7395 T7394 arg1Of stage,the
R6333 T7398 T7397 arg1Of Axiovert,Zeiss
R6334 T7398 T7399 arg1Of Axiovert,200
R6335 T7400 T7393 arg3Of microscope,on
R6336 T7400 T7396 arg1Of microscope,a
R6337 T7400 T7398 arg1Of microscope,Axiovert
R6338 T7402 T7401 arg2Of Thornwood,(
R6339 T7402 T7403 arg1Of Thornwood,","
R6340 T7405 T7403 arg2Of York,","
R6341 T7405 T7404 arg1Of York,New
R6342 T7405 T7406 arg1Of York,","
R6343 T7407 T7406 arg2Of USA,","
R6344 T7408 T7401 arg3Of ),(
R6345 T7409 T7410 arg1Of Observations,were
R6346 T7409 T7411 arg2Of Observations,made
R6347 T7411 T7410 arg2Of made,were
R6348 T7411 T7419 arg1Of made,and
R6349 T7417 T7411 arg1Of lens,made
R6350 T7417 T7412 arg2Of lens,by
R6351 T7417 T7413 arg1Of lens,40×1.0
R6352 T7417 T7414 arg1Of lens,NA
R6353 T7417 T7415 arg1Of lens,oil-immersion
R6354 T7417 T7416 arg1Of lens,objective
R6355 T7419 T7418 arg1Of and,","
R6356 T7420 T7421 arg1Of fluorescence,and
R6357 T7422 T7421 arg2Of phase,and
R6358 T7424 T7420 arg1Of images,fluorescence
R6359 T7424 T7422 arg1Of images,phase
R6360 T7424 T7423 arg1Of images,contrast
R6361 T7424 T7425 arg1Of images,were
R6362 T7424 T7426 arg2Of images,gathered
R6363 T7426 T7419 arg2Of gathered,and
R6364 T7426 T7425 arg2Of gathered,were
R6365 T7426 T7427 modOf gathered,using
R6366 T7427 T7431 arg2Of using,charged
R6367 T7430 T7428 arg1Of ORCA-ER,a
R6368 T7430 T7429 arg1Of ORCA-ER,Hamamatsu
R6369 T7430 T7431 arg1Of ORCA-ER,charged
R6370 T7434 T7427 arg2Of camera,using
R6371 T7434 T7432 arg2Of camera,coupled
R6372 T7434 T7433 arg1Of camera,device
R6373 T7434 T7435 arg1Of camera,(
R6374 T7434 T7443 arg2Of camera,driven
R6375 T7436 T7435 arg2Of Bridgewater,(
R6376 T7436 T7437 arg1Of Bridgewater,","
R6377 T7439 T7437 arg2Of Jersey,","
R6378 T7439 T7438 arg1Of Jersey,New
R6379 T7439 T7440 arg1Of Jersey,","
R6380 T7441 T7440 arg2Of USA,","
R6381 T7442 T7435 arg3Of ),(
R6382 T7443 T7447 arg1Of driven,(
R6383 T7446 T7443 arg1Of software,driven
R6384 T7446 T7444 arg2Of software,by
R6385 T7446 T7445 arg1Of software,Openlab
R6386 T7448 T7449 arg1Of Improvision,","
R6387 T7449 T7447 arg2Of ",",(
R6388 T7449 T7451 arg1Of ",",","
R6389 T7450 T7449 arg2Of Coventry,","
R6390 T7452 T7451 arg2Of UK,","
R6391 T7453 T7447 arg3Of ),(
R6392 T7454 T7455 arg1Of Photographs,were
R6393 T7454 T7456 arg2Of Photographs,taken
R6394 T7456 T7455 arg2Of taken,were
R6395 T7456 T7457 arg1Of taken,at
R6396 T7458 T7459 arg1Of 0,","
R6397 T7459 T7461 arg1Of ",",","
R6398 T7460 T7459 arg2Of 30,","
R6399 T7461 T7463 arg1Of ",",","
R6400 T7462 T7461 arg2Of 60,","
R6401 T7463 T7466 arg1Of ",",and
R6402 T7464 T7463 arg2Of 120,","
R6403 T7466 T7465 arg1Of and,","
R6404 T7467 T7466 arg2Of 240,and
R6405 T7468 T7457 arg2Of min,at
R6406 T7468 T7458 arg1Of min,0
R6407 T7468 T7460 arg1Of min,30
R6408 T7468 T7462 arg1Of min,60
R6409 T7468 T7464 arg1Of min,120
R6410 T7468 T7467 arg1Of min,240
R6533 T7612 T7611 arg1Of Analysis,Densitometric
R6534 T7613 T7614 arg1Of Densitometry,of
R6535 T7613 T7617 arg1Of Densitometry,was
R6536 T7613 T7618 arg2Of Densitometry,performed
R6537 T7616 T7614 arg2Of immunoblots,of
R6538 T7616 T7615 arg1Of immunoblots,ECL
R6539 T7618 T7617 arg2Of performed,was
R6540 T7619 T7618 arg3Of using,performed
R6541 T7621 T7620 arg1Of ID,Gelworks
R6542 T7623 T7619 arg2Of software,using
R6543 T7623 T7621 arg1Of software,ID
R6544 T7623 T7622 arg1Of software,intermediate
R6545 T7623 T7624 arg1Of software,(
R6546 T7626 T7624 arg2Of Products,(
R6547 T7626 T7625 arg1Of Products,Ultraviolet
R6548 T7626 T7627 arg1Of Products,","
R6549 T7628 T7627 arg2Of Cambridgeshire,","
R6550 T7628 T7629 arg1Of Cambridgeshire,","
R6551 T7630 T7629 arg2Of UK,","
R6552 T7631 T7624 arg3Of ),(
R6553 T7634 T7635 arg1Of immunoblots,were
R6554 T7634 T7636 arg2Of immunoblots,scanned
R6555 T7636 T7635 arg2Of scanned,were
R6556 T7636 T7637 arg1Of scanned,and
R6557 T7637 T7632 arg1Of and,Briefly
R6558 T7637 T7633 arg1Of and,","
R6559 T7638 T7639 arg1Of gates,were
R6560 T7638 T7640 arg2Of gates,drawn
R6561 T7640 T7637 arg2Of drawn,and
R6562 T7640 T7639 arg2Of drawn,were
R6563 T7640 T7641 arg1Of drawn,tightly
R6564 T7640 T7642 arg1Of drawn,around
R6565 T7644 T7642 arg2Of band,around
R6566 T7644 T7643 arg1Of band,each
R6567 T7646 T7645 arg1Of values,Background
R6568 T7646 T7647 arg1Of values,from
R6572 T7649 T7647 arg2Of lane,from
R6604 T7679 T7686 arg2Of films,selected
R6605 T7682 T7680 arg2Of levels,generating
R6606 T7682 T7681 arg1Of levels,subsaturating
R6607 T7682 T7683 arg1Of levels,of
R6608 T7684 T7683 arg2Of intensity,of
R6609 T7686 T7677 arg2Of selected,and
R6610 T7686 T7685 arg2Of selected,were
R6611 T7686 T7687 arg1Of selected,for
R6612 T7689 T7687 arg2Of evaluation,for
R6613 T7689 T7688 arg1Of evaluation,densitometric
R6698 T7788 T7787 arg1Of Analysis,Statistical
R6699 T7789 T7790 arg1Of Data,from
R6700 T7789 T7796 arg1Of Data,are
R6701 T7789 T7797 arg2Of Data,presented
R6702 T7789 T7813 arg1Of Data,were
R6703 T7789 T7814 arg2Of Data,compared
R6704 T7789 T7815 arg1Of Data,using
R6705 T7791 T7792 arg1Of three,or
R6706 T7791 T7793 arg1Of three,more
R6707 T7795 T7790 arg2Of experiments,from
R6708 T7795 T7791 arg1Of experiments,three
R6709 T7795 T7794 arg1Of experiments,independent
R6710 T7797 T7796 arg2Of presented,are
R6711 T7797 T7798 arg1Of presented,as
R6712 T7797 T7812 arg1Of presented,and
R6713 T7801 T7798 arg2Of error,as
R6714 T7801 T7799 arg1Of error,the
R6715 T7801 T7800 arg1Of error,mean±standard
R6716 T7801 T7802 arg1Of error,of
R6717 T7801 T7808 arg1Of error,","
R6718 T7801 T7809 arg1Of error,except
R6719 T7801 T7811 arg2Of error,stated
R6720 T7804 T7802 arg2Of mean,of
R6721 T7804 T7803 arg1Of mean,the
R6722 T7804 T7805 arg1Of mean,(
R6723 T7806 T7805 arg2Of SEM,(
R6724 T7807 T7805 arg3Of ),(
R6725 T7811 T7810 arg1Of stated,where
R6726 T7814 T7812 arg2Of compared,and
R6727 T7814 T7813 arg2Of compared,were
R6728 T7815 T7814 arg3Of using,compared
R6729 T7815 T7821 arg1Of using,Software
R6730 T7815 T7825 arg1Of using,//www.graphpad.com
R6731 T7817 T7815 arg2Of Prism,using
R6732 T7817 T7816 arg1Of Prism,GraphPad
R6733 T7817 T7818 arg1Of Prism,4
R6734 T7821 T7820 arg1Of Software,GraphPad
R6735 T7821 T7822 arg1Of Software,","
R6736 T7822 T7819 arg1Of ",",(
R6737 T7822 T7826 arg1Of ",",)
R6738 T7823 T7824 arg1Of http,:
R6739 T7825 T7822 arg2Of //www.graphpad.com,","
R6740 T7825 T7823 arg1Of //www.graphpad.com,http
R6741 T7827 T7828 arg1Of Results,were
R6742 T7827 T7829 arg2Of Results,analysed
R6743 T7829 T7828 arg2Of analysed,were
R6744 T7829 T7830 modOf analysed,using
R6745 T7830 T7833 arg1Of using,with
R6746 T7832 T7830 arg2Of ANOVA,using
R6747 T7832 T7831 arg1Of ANOVA,one-way
R6748 T7834 T7833 arg2Of Newman-Keuls,with
R6749 T7836 T7835 arg1Of test,post
R6750 T7836 T7837 arg1Of test,except
R6751 T7836 T7859 arg2Of test,matched
R6752 T7836 T7861 arg2Of test,signed
R6753 T7837 T7838 arg1Of except,for
R6754 T7840 T7839 arg1Of data,the
R6755 T7840 T7841 arg1Of data,from
R6756 T7840 T7859 arg1Of data,matched
R6757 T7844 T7843 arg1Of vivo,in
R6758 T7845 T7844 arg1Of inhaled,vivo
R6759 T7847 T7841 arg2Of study,from
R6760 T7847 T7842 arg1Of study,the
R6761 T7847 T7845 arg1Of study,inhaled
R6762 T7847 T7846 arg1Of study,FP
R6763 T7847 T7848 arg1Of study,","
R6764 T7847 T7849 arg1Of study,which
R6765 T7847 T7850 arg1Of study,was
R6766 T7847 T7851 arg2Of study,analysed
R6767 T7851 T7850 arg2Of analysed,was
R6768 T7853 T7854 arg2Of Friedman,'s
R6769 T7855 T7851 arg1Of test,analysed
R6770 T7855 T7852 arg2Of test,by
R6771 T7855 T7854 arg1Of test,'s
R6772 T7855 T7856 arg1Of test,with
R6773 T7858 T7856 arg2Of Wilcoxson,with
R6774 T7858 T7857 arg1Of Wilcoxson,subsequent
R6775 T7860 T7861 arg1Of pair,signed
R6776 T7861 T7828 modOf signed,were
R6777 T7864 T7861 arg3Of test,signed
R6778 T7864 T7862 arg1Of test,rank
R6779 T7864 T7863 arg1Of test,sum
R6780 T7865 T7866 arg1Of Data,from
R6781 T7865 T7869 arg1Of Data,are
R6782 T7865 T7870 arg2Of Data,presented
R6783 T7868 T7866 arg2Of analysis,from
R6784 T7868 T7867 arg1Of analysis,this
R6785 T7870 T7869 arg2Of presented,are
R6786 T7870 T7871 arg1Of presented,as
R6787 T7874 T7871 arg2Of plot,as
R6788 T7874 T7872 arg1Of plot,a
R6789 T7874 T7873 arg1Of plot,box-and-whiskers
R6790 T7875 T7876 arg2Of Friedman,'s
R6791 T7877 T7876 arg1Of test,'s
R6792 T7877 T7878 arg1Of test,was
R6793 T7877 T7879 arg2Of test,used
R6794 T7879 T7878 arg2Of used,was
R6795 T7879 T7880 arg1Of used,as
R6796 T7883 T7881 arg1Of measures,three
R6797 T7883 T7882 arg1Of measures,matched
R6798 T7883 T7884 arg1Of measures,were
R6799 T7883 T7885 arg2Of measures,obtained
R6800 T7883 T7886 arg1Of measures,using
R6801 T7885 T7880 arg2Of obtained,as
R6802 T7885 T7884 arg2Of obtained,were
R6803 T7886 T7885 arg3Of using,obtained
R6804 T7887 T7888 arg1Of placebo,","
R6805 T7888 T7892 arg1Of ",",and
R6806 T7890 T7888 arg2Of µg,","
R6807 T7890 T7889 arg1Of µg,100
R6808 T7892 T7886 arg2Of and,using
R6809 T7892 T7891 arg1Of and,","
R6810 T7894 T7892 arg2Of µg,and
R6811 T7894 T7893 arg1Of µg,500
R6812 T7894 T7895 arg1Of µg,of
R6813 T7894 T7898 arg1Of µg,","
R6814 T7894 T7899 arg1Of µg,which
R6815 T7894 T7900 arg1Of µg,had
R6816 T7897 T7895 arg2Of FP,of
R6817 T7897 T7896 arg2Of FP,inhaled
R6818 T7903 T7900 arg2Of levels,had
R6819 T7903 T7901 arg1Of levels,variable
R6820 T7903 T7902 arg1Of levels,baseline
R6821 T7904 T7905 arg1Of We,did
R6822 T7904 T7907 arg1Of We,assume
R6823 T7907 T7905 arg2Of assume,did
R6824 T7907 T7906 arg1Of assume,not
R6825 T7910 T7907 arg2Of distribution,assume
R6826 T7910 T7908 arg1Of distribution,a
R6827 T7910 T7909 arg1Of distribution,Gaussian
R6828 T7910 T7911 arg1Of distribution,of
R6829 T7910 T7922 arg1Of distribution,(
R6830 T7913 T7911 arg2Of data,of
R6831 T7913 T7912 arg1Of data,the
R6832 T7913 T7914 arg1Of data,due
R6833 T7914 T7915 arg1Of due,to
R6834 T7918 T7915 arg2Of numbers,to
R6835 T7918 T7916 arg1Of numbers,the
R6836 T7918 T7917 arg1Of numbers,limited
R6837 T7918 T7919 arg1Of numbers,of
R6838 T7920 T7919 arg2Of participants,of
R6839 T7920 T7921 arg2Of participants,analysed
R6840 T7923 T7922 arg2Of seven,(
R6841 T7924 T7922 arg3Of ),(
R6842 T7927 T7925 arg1Of hypothesis,.The
R6843 T7927 T7926 arg1Of hypothesis,null
R6844 T7927 T7928 arg1Of hypothesis,was
R6845 T7927 T7929 arg2Of hypothesis,rejected
R6846 T7929 T7907 arg3Of rejected,assume
R6847 T7929 T7928 arg2Of rejected,was
R6848 T7929 T7930 arg1Of rejected,at
R6849 T7933 T7930 arg2Of 0.05,at
R6850 T7933 T7931 arg1Of 0.05,p
R6851 T7933 T7932 arg1Of 0.05,<
R3974 T4628 T4629 modOf extracted,using
R3976 T4631 T4629 arg2Of buffer,using
R3977 T4631 T4630 arg1Of buffer,lysis
R3978 T4631 T4632 arg1Of buffer,(
R6569 T7646 T7650 arg1Of values,were
R6570 T7646 T7651 arg2Of values,subtracted
R6571 T7646 T7653 arg1Of values,normalize
R6573 T7649 T7648 arg1Of lane,each
R6574 T7651 T7650 arg2Of subtracted,were
R6575 T7653 T7651 arg3Of normalize,subtracted
R6576 T7653 T7652 arg1Of normalize,to
R6577 T7655 T7653 arg2Of measurement,normalize
R6578 T7655 T7654 arg1Of measurement,each
R6579 T7657 T7656 arg1Of bands,The
R6580 T7657 T7658 arg1Of bands,were
R6581 T7657 T7659 arg2Of bands,quantified
R6582 T7659 T7658 arg2Of quantified,were
R6583 T7660 T7659 arg3Of using,quantified
R6584 T7663 T7660 arg2Of software,using
R6585 T7663 T7661 arg1Of software,the
R6586 T7663 T7662 arg1Of software,Gelworks
R6587 T7666 T7664 arg1Of blots,All
R6588 T7666 T7665 arg1Of blots,Western
R6589 T7666 T7667 arg1Of blots,were
R6590 T7666 T7668 arg2Of blots,exposed
R6591 T7668 T7667 arg2Of exposed,were
R6592 T7668 T7669 modOf exposed,to
R6593 T7668 T7677 arg1Of exposed,and
R6594 T7670 T7669 arg1Of film,to
R6595 T7670 T7671 arg1Of film,for
R6596 T7673 T7671 arg2Of lengths,for
R6597 T7673 T7672 arg1Of lengths,varying
R6598 T7673 T7674 arg1Of lengths,of
R6599 T7675 T7674 arg2Of time,of
R6600 T7677 T7676 arg1Of and,","
R6601 T7679 T7678 arg1Of films,only
R6602 T7679 T7680 arg1Of films,generating
R6603 T7679 T7685 arg1Of films,were

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T3810 23-35 NNS denotes Participants
T3811 36-39 CC denotes and
T3812 40-45 NNP denotes Study
T3813 46-52 NNP denotes Design
T3814 53-55 PRP denotes We
T3815 56-63 VBD denotes studied
T3816 64-72 NNS denotes patients
T3817 73-77 IN denotes with
T3818 78-82 JJ denotes mild
T3819 83-89 NN denotes asthma
T3820 90-93 WP denotes who
T3821 94-98 VBD denotes were
T3822 99-102 RB denotes not
T3823 103-110 VBN denotes treated
T3824 111-115 IN denotes with
T3825 116-123 JJ denotes inhaled
T3826 124-139 NNS denotes corticosteroids
T3827 140-143 WP denotes who
T3828 144-147 VBD denotes had
T3829 148-152 VBN denotes been
T3830 153-161 VBN denotes included
T3831 162-164 IN denotes in
T3832 165-166 DT denotes a
T3833 167-177 RB denotes previously
T3834 178-186 VBN denotes reported
T3835 187-199 NN denotes double-blind
T3836 199-200 , denotes ,
T3837 201-219 JJ denotes placebo-controlled
T3838 219-220 , denotes ,
T3839 221-230 JJ denotes crossover
T3840 231-236 NN denotes study
T3841 237-241 IN denotes with
T3842 242-244 NNP denotes FP
T3843 245-246 NNP denotes [
T3844 246-248 CD denotes 34
T3845 248-249 NNP denotes ]
T3846 249-250 . denotes .
T3847 251-256 CD denotes Seven
T3848 257-265 NNS denotes patients
T3849 266-270 IN denotes with
T3850 271-275 JJ denotes mild
T3851 276-282 NN denotes asthma
T3852 283-290 VBD denotes entered
T3853 291-294 DT denotes the
T3854 295-300 NN denotes study
T3855 301-304 CC denotes and
T3856 305-309 VBD denotes were
T3857 310-320 VBN denotes randomized
T3858 321-323 TO denotes to
T3859 324-331 VB denotes receive
T3860 332-333 DT denotes a
T3861 334-340 JJ denotes single
T3862 341-351 NN denotes inhalation
T3863 352-354 IN denotes of
T3864 355-357 NNP denotes FP
T3865 358-359 -LRB- denotes (
T3866 359-362 CD denotes 100
T3867 363-366 CC denotes and
T3868 367-370 CD denotes 500
T3869 371-373 NN denotes µg
T3870 373-374 -RRB- denotes )
T3871 375-377 CC denotes or
T3872 378-379 DT denotes a
T3873 380-387 VBN denotes matched
T3874 388-395 NN denotes placebo
T3875 396-403 NN denotes control
T3876 404-407 IN denotes via
T3877 408-409 DT denotes a
T3878 410-416 NN denotes spacer
T3879 417-424 NN denotes chamber
T3880 424-425 , denotes ,
T3881 426-429 CC denotes and
T3882 430-433 DT denotes the
T3883 434-439 JJ denotes other
T3884 440-449 NN denotes treatment
T3885 450-453 VBD denotes was
T3886 454-459 VBN denotes given
T3887 460-465 IN denotes after
T3888 466-467 DT denotes a
T3889 468-476 JJ denotes wash-out
T3890 477-483 NN denotes period
T3891 484-486 IN denotes of
T3892 487-489 IN denotes at
T3893 490-495 JJS denotes least
T3894 496-497 CD denotes 6
T3895 498-500 NN denotes d.
T3896 501-506 NNP denotes Blood
T3897 507-510 VBD denotes was
T3898 511-516 VBN denotes taken
T3899 517-520 IN denotes for
T3900 521-532 NN denotes preparation
T3901 533-535 IN denotes of
T3902 536-541 NNS denotes PBMCs
T3903 542-544 IN denotes at
T3904 545-546 CD denotes 1
T3905 547-550 CC denotes and
T3906 551-552 CD denotes 2
T3907 553-554 NN denotes h
T3908 555-560 IN denotes after
T3909 561-565 NN denotes drug
T3910 566-580 NN denotes administration
T3911 580-581 . denotes .
T3912 582-585 DT denotes All
T3913 586-594 NNS denotes patients
T3914 595-599 VBD denotes gave
T3915 600-608 VBN denotes informed
T3916 609-616 NN denotes consent
T3917 617-620 CC denotes and
T3918 621-624 DT denotes the
T3919 625-630 NN denotes study
T3920 631-634 VBD denotes was
T3921 635-643 VBN denotes approved
T3922 644-646 IN denotes by
T3923 647-650 DT denotes the
T3924 651-657 NNPS denotes Ethics
T3925 658-667 NNP denotes Committee
T3926 668-670 IN denotes of
T3927 671-674 DT denotes the
T3928 675-680 NNP denotes Royal
T3929 681-689 NNP denotes Brompton
T3930 690-693 CC denotes and
T3931 694-703 NNP denotes Harefield
T3932 704-713 NNPS denotes Hospitals
T3933 714-717 NNP denotes NHS
T3934 718-723 NNP denotes Trust
T3935 723-724 . denotes .
T3936 725-728 DT denotes The
T3937 729-737 JJ denotes clinical
T3938 738-743 NN denotes study
T3939 744-747 VBD denotes was
T3940 748-757 VBN denotes conducted
T3941 758-764 IN denotes before
T3942 765-768 DT denotes the
T3943 769-780 NN denotes requirement
T3944 781-784 IN denotes for
T3945 785-793 NNP denotes Clinical
T3946 794-799 NNP denotes Trial
T3947 800-812 NNP denotes Registration
T3948 812-813 . denotes .
T4142 815-825 NNS denotes Antibodies
T4143 826-829 CC denotes and
T4144 830-838 NNS denotes Reagents
T4145 839-842 DT denotes The
T4146 843-853 JJ denotes monoclonal
T4147 854-864 NNS denotes antibodies
T4148 865-872 IN denotes against
T4149 873-878 JJ denotes human
T4150 879-882 NNP denotes CD3
T4151 882-883 , denotes ,
T4152 884-888 NNP denotes CD28
T4153 888-889 , denotes ,
T4154 890-892 NNP denotes GR
T4155 892-893 , denotes ,
T4156 894-897 CC denotes and
T4157 898-908 JJ denotes importin-α
T4158 909-913 VBD denotes were
T4159 914-923 VBN denotes purchased
T4160 924-928 IN denotes from
T4161 929-931 NNP denotes BD
T4162 932-943 NNP denotes Biosciences
T4163 944-945 -LRB- denotes (
T4164 945-951 NNP denotes Oxford
T4165 951-952 , denotes ,
T4166 953-959 NNP denotes United
T4167 960-967 NNP denotes Kingdom
T4168 967-968 -RRB- denotes )
T4169 968-969 . denotes .
T4170 970-976 NN denotes Rabbit
T4171 977-987 NNS denotes antibodies
T4172 988-995 IN denotes against
T4173 996-1001 JJ denotes human
T4174 1002-1008 NNP denotes GATA-3
T4175 1009-1010 -LRB- denotes (
T4176 1010-1014 NNP denotes H-48
T4177 1014-1015 -RRB- denotes )
T4178 1016-1019 CC denotes and
T4179 1020-1022 NNP denotes GR
T4180 1023-1024 -LRB- denotes (
T4181 1024-1028 NNP denotes E-20
T4182 1028-1029 , denotes ,
T4183 1030-1037 JJ denotes sc-1003
T4184 1037-1038 -RRB- denotes )
T4185 1039-1043 VBD denotes were
T4186 1044-1052 VBN denotes obtained
T4187 1053-1057 IN denotes from
T4188 1058-1063 NNP denotes Santa
T4189 1064-1068 NNP denotes Cruz
T4190 1069-1082 NNP denotes Biotechnology
T4191 1083-1084 -LRB- denotes (
T4192 1084-1089 NNP denotes Santa
T4193 1090-1094 NNP denotes Cruz
T4194 1094-1095 , denotes ,
T4195 1096-1106 NNP denotes California
T4196 1106-1107 , denotes ,
T4197 1108-1114 NNP denotes United
T4198 1115-1121 NNPS denotes States
T4199 1121-1122 -RRB- denotes )
T4200 1122-1123 , denotes ,
T4201 1124-1134 JJ denotes polyclonal
T4202 1135-1141 NN denotes rabbit
T4203 1142-1152 NNS denotes antibodies
T4204 1153-1160 IN denotes against
T4205 1161-1172 JJ denotes phospho-p38
T4206 1173-1176 NNP denotes MAP
T4207 1177-1183 NN denotes kinase
T4208 1184-1187 CC denotes and
T4209 1188-1201 NN denotes phospho-ATF-2
T4210 1202-1206 IN denotes from
T4211 1207-1211 NNP denotes Cell
T4212 1212-1221 NNP denotes Signaling
T4213 1222-1232 NNP denotes Technology
T4214 1233-1234 -LRB- denotes (
T4215 1234-1237 NNP denotes New
T4216 1238-1245 NNP denotes England
T4217 1246-1253 NNPS denotes Biolabs
T4218 1253-1254 , denotes ,
T4219 1255-1263 NNP denotes Hertford
T4220 1263-1264 , denotes ,
T4221 1265-1267 NNP denotes UK
T4222 1267-1268 -RRB- denotes )
T4223 1268-1269 , denotes ,
T4224 1270-1280 JJ denotes monoclonal
T4225 1281-1289 NN denotes antibody
T4226 1290-1297 IN denotes against
T4227 1298-1311 NN denotes phosphoserine
T4228 1312-1313 -LRB- denotes (
T4229 1313-1318 JJ denotes clone
T4230 1319-1322 NNP denotes 4H4
T4231 1322-1323 -RRB- denotes )
T4232 1324-1328 IN denotes from
T4233 1329-1337 NNP denotes Affiniti
T4234 1338-1346 NNP denotes Research
T4235 1347-1355 NNP denotes Products
T4236 1356-1357 -LRB- denotes (
T4237 1357-1363 NNP denotes Exeter
T4238 1363-1364 , denotes ,
T4239 1365-1367 NNP denotes UK
T4240 1367-1368 -RRB- denotes )
T4241 1368-1369 , denotes ,
T4242 1370-1373 CC denotes and
T4243 1374-1384 NNS denotes antibodies
T4244 1385-1392 IN denotes against
T4245 1393-1399 NN denotes rabbit
T4246 1400-1414 NNP denotes IgG-conjugated
T4247 1415-1420 NNP denotes TRITC
T4248 1421-1425 IN denotes from
T4249 1426-1430 NNP denotes Dako
T4250 1431-1441 NNP denotes Cytomation
T4251 1442-1443 -LRB- denotes (
T4252 1443-1452 NNP denotes Cambridge
T4253 1452-1453 , denotes ,
T4254 1454-1456 NNP denotes UK
T4255 1456-1457 -RRB- denotes )
T4256 1457-1458 . denotes .
T4257 1459-1462 DT denotes The
T4258 1463-1470 JJ denotes anti-GR
T4259 1471-1479 NN denotes antibody
T4260 1480-1481 -LRB- denotes (
T4261 1481-1486 NNP denotes Clone
T4262 1487-1489 CD denotes 41
T4263 1489-1490 -RRB- denotes )
T4264 1491-1495 IN denotes from
T4265 1496-1498 NNP denotes BD
T4266 1499-1511 NNP denotes Transduction
T4267 1512-1524 NNP denotes Laboratories
T4268 1525-1526 -LRB- denotes (
T4269 1526-1532 NNP denotes Oxford
T4270 1532-1533 , denotes ,
T4271 1534-1536 NNP denotes UK
T4272 1536-1537 -RRB- denotes )
T4273 1538-1541 VBD denotes was
T4274 1542-1546 VBN denotes used
T4275 1547-1550 IN denotes for
T4276 1551-1558 JJ denotes Western
T4277 1559-1563 NN denotes blot
T4278 1564-1572 NN denotes analysis
T4279 1572-1573 . denotes .
T4280 1574-1577 DT denotes All
T4281 1578-1583 JJ denotes other
T4282 1584-1592 NNS denotes reagents
T4283 1593-1597 VBD denotes were
T4284 1598-1607 VBN denotes purchased
T4285 1608-1612 IN denotes from
T4286 1613-1618 NNP denotes Sigma
T4287 1619-1620 -LRB- denotes (
T4288 1620-1625 NNP denotes Poole
T4289 1625-1626 , denotes ,
T4290 1627-1629 NNP denotes UK
T4291 1629-1630 -RRB- denotes )
T4292 1630-1631 . denotes .
T4470 1633-1637 NNP denotes Cell
T4471 1638-1645 NNP denotes Culture
T4472 1646-1649 CC denotes and
T4473 1650-1654 NNP denotes PBMC
T4474 1655-1664 NNP denotes Isolation
T4475 1665-1666 NNP denotes A
T4476 1667-1672 JJ denotes human
T4477 1673-1674 NNP denotes T
T4478 1675-1679 NN denotes cell
T4479 1680-1684 NN denotes line
T4480 1685-1686 -LRB- denotes (
T4481 1686-1692 JJ denotes HuT-78
T4482 1692-1693 -RRB- denotes )
T4483 1694-1697 VBD denotes was
T4484 1698-1707 VBN denotes purchased
T4485 1708-1712 IN denotes from
T4486 1713-1718 NNP denotes ECACC
T4487 1719-1727 NNP denotes European
T4488 1728-1738 NNP denotes Collection
T4489 1739-1741 IN denotes of
T4490 1742-1746 NNP denotes Cell
T4491 1747-1754 NNP denotes Culture
T4492 1755-1756 -LRB- denotes (
T4493 1756-1765 NNP denotes Wiltshire
T4494 1765-1766 , denotes ,
T4495 1767-1769 NNP denotes UK
T4496 1769-1770 -RRB- denotes )
T4497 1771-1775 VBD denotes were
T4498 1776-1784 VBN denotes cultured
T4499 1785-1787 IN denotes as
T4500 1788-1798 RB denotes previously
T4501 1799-1808 VBN denotes described
T4502 1809-1810 NNP denotes [
T4503 1810-1812 CD denotes 12
T4504 1812-1813 NNP denotes ]
T4505 1813-1814 . denotes .
T4506 1815-1820 NNS denotes PBMCs
T4507 1821-1825 VBD denotes were
T4508 1826-1834 VBN denotes isolated
T4509 1835-1837 IN denotes by
T4510 1838-1845 NN denotes density
T4511 1846-1860 NN denotes centrifugation
T4512 1861-1865 IN denotes over
T4513 1866-1880 NNP denotes Ficoll-Hypaque
T4514 1881-1882 -LRB- denotes (
T4515 1882-1889 NN denotes density
T4516 1889-1890 , denotes ,
T4517 1891-1896 CD denotes 1.077
T4518 1897-1901 NN denotes g/ml
T4519 1901-1902 : denotes ;
T4520 1903-1911 NNP denotes Amersham
T4521 1912-1923 NNP denotes Biosciences
T4522 1923-1924 , denotes ,
T4523 1925-1933 NNP denotes Amersham
T4524 1933-1934 , denotes ,
T4525 1935-1937 NNP denotes UK
T4526 1937-1938 -RRB- denotes )
T4527 1939-1941 IN denotes as
T4528 1942-1952 RB denotes previously
T4529 1953-1962 VBN denotes described
T4530 1963-1964 NNP denotes [
T4531 1964-1966 CD denotes 34
T4532 1966-1967 NNP denotes ]
T4533 1967-1968 . denotes .
T4534 1969-1974 NNS denotes Cells
T4535 1975-1979 VBD denotes were
T4536 1980-1990 VBN denotes stimulated
T4537 1991-1995 IN denotes with
T4538 1996-2004 JJ denotes anti-CD3
T4539 2004-2005 NN denotes /
T4540 2005-2009 NNP denotes CD28
T4541 2010-2011 -LRB- denotes (
T4542 2011-2012 CD denotes 1
T4543 2013-2015 NN denotes µg
T4544 2015-2016 NN denotes /
T4545 2016-2018 NN denotes ml
T4546 2019-2023 DT denotes each
T4547 2023-2024 -RRB- denotes )
T4548 2025-2028 IN denotes for
T4549 2029-2030 CD denotes 1
T4550 2031-2032 NN denotes h
T4551 2033-2035 IN denotes at
T4552 2036-2038 CD denotes 37
T4553 2038-2039 CD denotes °
T4554 2039-2040 NNP denotes C
T4555 2041-2043 TO denotes to
T4556 2044-2053 VB denotes stimulate
T4557 2054-2057 JJ denotes Th2
T4558 2058-2066 NN denotes cytokine
T4559 2067-2074 NN denotes release
T4560 2075-2077 IN denotes in
T4561 2078-2081 DT denotes the
T4562 2082-2090 NN denotes presence
T4563 2091-2093 CC denotes or
T4564 2094-2101 NN denotes absence
T4565 2102-2104 IN denotes of
T4566 2105-2107 NNP denotes FP
T4567 2108-2109 -LRB- denotes (
T4568 2109-2111 CD denotes 10
T4569 2111-2112 NN denotes −
T4570 2112-2114 CD denotes 12
T4571 2115-2117 TO denotes to
T4572 2118-2120 CD denotes 10
T4573 2120-2121 CD denotes −
T4574 2121-2123 NNP denotes 8M
T4575 2123-2124 -RRB- denotes )
T4576 2124-2125 . denotes .
T4577 2126-2135 NNS denotes Cytospins
T4578 2136-2140 VBD denotes were
T4579 2141-2149 JJ denotes prepared
T4580 2150-2153 CC denotes and
T4581 2154-2160 JJ denotes GATA-3
T4582 2161-2173 NN denotes localization
T4583 2174-2184 VBN denotes determined
T4584 2185-2187 IN denotes by
T4585 2188-2196 JJ denotes confocal
T4586 2197-2207 NN denotes microscopy
T4587 2208-2210 IN denotes as
T4588 2211-2221 RB denotes previously
T4589 2222-2231 VBN denotes described
T4590 2232-2233 NNP denotes [
T4591 2233-2235 CD denotes 12
T4592 2235-2236 NNP denotes ]
T4593 2236-2237 . denotes .
T4717 2239-2246 VB denotes Reverse
T4718 2247-2260 NNP denotes Transcription
T4719 2261-2264 NNP denotes PCR
T4720 2265-2270 NNP denotes Total
T4721 2271-2274 NNP denotes RNA
T4722 2275-2278 VBD denotes was
T4723 2279-2288 VBN denotes extracted
T4724 2289-2294 VBG denotes using
T4725 2295-2300 NN denotes lysis
T4726 2301-2307 NN denotes buffer
T4727 2308-2309 -LRB- denotes (
T4728 2309-2315 NNP denotes RNeasy
T4729 2316-2319 NN denotes kit
T4730 2319-2320 : denotes ;
T4731 2321-2327 NNP denotes Qiagen
T4732 2327-2328 , denotes ,
T4733 2329-2336 NNP denotes Crawley
T4734 2336-2337 , denotes ,
T4735 2338-2340 NNP denotes UK
T4736 2340-2341 -RRB- denotes )
T4737 2341-2342 . denotes .
T4738 2343-2349 IN denotes During
T4739 2350-2353 NNP denotes RNA
T4740 2354-2366 NN denotes purification
T4741 2366-2367 , denotes ,
T4742 2368-2375 JJ denotes genomic
T4743 2376-2379 NN denotes DNA
T4744 2380-2383 VBD denotes was
T4745 2384-2392 VBN denotes digested
T4746 2393-2397 IN denotes with
T4747 2398-2408 JJ denotes RNase-free
T4748 2409-2414 NNP denotes DNase
T4749 2415-2416 -LRB- denotes (
T4750 2416-2424 NNP denotes Amersham
T4751 2425-2436 NNPS denotes Biosciences
T4752 2436-2437 -RRB- denotes )
T4753 2437-2438 . denotes .
T4754 2439-2443 JJ denotes Next
T4755 2443-2444 , denotes ,
T4756 2445-2448 CD denotes 0.5
T4757 2449-2451 NN denotes µg
T4758 2452-2454 IN denotes of
T4759 2455-2460 JJ denotes total
T4760 2461-2464 NNP denotes RNA
T4761 2465-2468 VBD denotes was
T4762 2469-2477 JJ denotes reversed
T4763 2478-2489 VBN denotes transcribed
T4764 2490-2495 VBG denotes using
T4765 2496-2499 DT denotes the
T4766 2500-2505 JJ denotes avian
T4767 2506-2520 NN denotes myeloblastosis
T4768 2521-2526 NN denotes virus
T4769 2527-2529 NNP denotes RT
T4770 2530-2531 -LRB- denotes (
T4771 2531-2538 NNP denotes Promega
T4772 2538-2539 , denotes ,
T4773 2540-2551 NNP denotes Southampton
T4774 2551-2552 , denotes ,
T4775 2553-2555 NNP denotes UK
T4776 2555-2556 -RRB- denotes )
T4777 2556-2557 . denotes .
T4778 2558-2561 IN denotes For
T4779 2562-2570 JJ denotes relative
T4780 2571-2585 NN denotes quantification
T4781 2585-2586 , denotes ,
T4782 2587-2593 NN denotes RT-PCR
T4783 2594-2597 VBD denotes was
T4784 2598-2605 VBN denotes carried
T4785 2606-2609 IN denotes out
T4786 2610-2615 VBG denotes using
T4787 2616-2620 NNP denotes cDNA
T4788 2621-2627 NNS denotes probes
T4789 2627-2628 . denotes .
T4790 2629-2636 NNS denotes Primers
T4791 2637-2640 IN denotes for
T4792 2641-2645 NN denotes IL-4
T4793 2646-2650 VBD denotes were
T4794 2651-2655 IN denotes from
T4795 2656-2669 NNP denotes Sigma-Genosys
T4796 2670-2671 -LRB- denotes (
T4797 2671-2680 NNP denotes Cambridge
T4798 2680-2681 , denotes ,
T4799 2682-2684 NNP denotes UK
T4800 2684-2685 -RRB- denotes )
T4801 2685-2686 . denotes .
T4802 2687-2696 NNS denotes Sequences
T4803 2697-2699 IN denotes of
T4804 2700-2705 NNP denotes GADPH
T4805 2706-2710 VBD denotes used
T4806 2711-2714 VBP denotes are
T4807 2715-2717 IN denotes as
T4808 2718-2725 VBZ denotes follows
T4809 2725-2726 : denotes :
T4810 2727-2734 RB denotes forward
T4811 2735-2736 CD denotes 5
T4812 2736-2737 SYM denotes ′
T4813 2737-2738 : denotes -
T4814 2738-2761 NNP denotes CCACCCATGGCAAATTCCATGGC
T4815 2761-2762 , denotes ,
T4816 2763-2770 VB denotes reverse
T4817 2771-2772 CD denotes 3
T4818 2772-2773 SYM denotes ′
T4819 2773-2774 : denotes -
T4820 2774-2797 NNP denotes TCTAGACGGCAGGTCAGGTCCAC
T4821 2797-2798 . denotes .
T5013 2800-2804 NNP denotes Cell
T5014 2805-2818 NNP denotes Fractionation
T5015 2818-2819 , denotes ,
T5016 2820-2839 NNP denotes Immunoprecipitation
T5017 2839-2840 , denotes ,
T5018 2841-2844 CC denotes and
T5019 2845-2852 JJ denotes Western
T5020 2853-2857 NNP denotes Blot
T5021 2858-2866 NNP denotes Analysis
T5022 2867-2874 NNP denotes Nuclear
T5023 2875-2878 CC denotes and
T5024 2879-2890 JJ denotes cytoplasmic
T5025 2891-2900 NNS denotes fractions
T5026 2901-2905 VBD denotes were
T5027 2906-2914 VBN denotes prepared
T5028 2915-2917 IN denotes as
T5029 2918-2928 RB denotes previously
T5030 2929-2938 VBN denotes described
T5031 2939-2940 NNP denotes [
T5032 2940-2942 CD denotes 35
T5033 2942-2943 NNP denotes ]
T5034 2943-2944 . denotes .
T5035 2945-2950 JJ denotes Whole
T5036 2951-2955 NN denotes cell
T5037 2956-2963 NNS denotes lysates
T5038 2964-2968 VBD denotes were
T5039 2969-2977 VBN denotes prepared
T5040 2978-2980 IN denotes in
T5041 2981-2986 NN denotes NP-40
T5042 2987-2992 NN denotes lysis
T5043 2993-2999 NN denotes buffer
T5044 3000-3001 -LRB- denotes (
T5045 3001-3004 CD denotes 0.5
T5046 3004-3005 NN denotes %
T5047 3006-3013 NNP denotes Nonidet
T5048 3014-3018 NNP denotes P-40
T5049 3018-3019 , denotes ,
T5050 3020-3022 CD denotes 20
T5051 3023-3025 NNP denotes mM
T5052 3026-3034 NNP denotes Tris-HCl
T5053 3035-3036 NNP denotes [
T5054 3036-3038 NNP denotes pH
T5055 3039-3042 CD denotes 7.5
T5056 3042-3043 NNP denotes ]
T5057 3043-3044 , denotes ,
T5058 3045-3048 CD denotes 150
T5059 3049-3051 NNP denotes mM
T5060 3052-3056 NNP denotes NaCl
T5061 3056-3057 -RRB- denotes )
T5062 3058-3060 IN denotes in
T5063 3061-3064 DT denotes the
T5064 3065-3073 NN denotes presence
T5065 3074-3076 IN denotes of
T5066 3077-3085 JJ denotes complete
T5067 3086-3094 NN denotes protease
T5068 3095-3103 NN denotes cocktail
T5069 3104-3113 NN denotes inhibitor
T5070 3113-3114 . denotes .
T5071 3115-3122 NNS denotes Lysates
T5072 3123-3127 VBD denotes were
T5073 3128-3139 VBN denotes centrifuged
T5074 3140-3142 IN denotes at
T5075 3143-3144 CD denotes 4
T5076 3144-3145 CD denotes °
T5077 3145-3146 NNP denotes C
T5078 3147-3150 IN denotes for
T5079 3151-3153 CD denotes 10
T5080 3154-3157 NN denotes min
T5081 3158-3160 IN denotes at
T5082 3161-3167 CD denotes 12,000
T5083 3168-3171 NN denotes rpm
T5084 3172-3174 IN denotes in
T5085 3175-3177 DT denotes an
T5086 3178-3187 NNP denotes Eppendorf
T5087 3188-3203 NN denotes microcentrifuge
T5088 3204-3206 TO denotes to
T5089 3207-3213 VB denotes remove
T5090 3214-3222 JJ denotes cellular
T5091 3223-3229 NN denotes debris
T5092 3229-3230 . denotes .
T5093 3231-3238 NNS denotes Samples
T5094 3239-3243 VBD denotes were
T5095 3244-3248 RB denotes then
T5096 3249-3267 VBN denotes immunoprecipitated
T5097 3268-3272 IN denotes with
T5098 3273-3279 DT denotes either
T5099 3280-3282 CD denotes 10
T5100 3283-3285 NN denotes µl
T5101 3286-3288 IN denotes of
T5102 3289-3297 NN denotes antibody
T5103 3298-3305 IN denotes against
T5104 3306-3312 NNP denotes GATA-3
T5105 3313-3315 CC denotes or
T5106 3316-3326 JJ denotes importin-α
T5107 3327-3332 VBG denotes using
T5108 3333-3336 NNP denotes A/G
T5109 3337-3344 JJ denotes agarose
T5110 3345-3351 NN denotes slurry
T5111 3352-3354 IN denotes in
T5112 3355-3358 DT denotes the
T5113 3359-3367 NN denotes presence
T5114 3368-3370 IN denotes of
T5115 3371-3379 NN denotes protease
T5116 3380-3389 NN denotes inhibitor
T5117 3390-3395 VBG denotes using
T5118 3396-3399 DT denotes the
T5119 3400-3405 NNP denotes Catch
T5120 3406-3409 CC denotes and
T5121 3410-3417 NNP denotes Release
T5122 3418-3429 NN denotes methodology
T5123 3430-3431 -LRB- denotes (
T5124 3431-3438 NNP denotes Upstate
T5125 3439-3452 NNP denotes Biotechnology
T5126 3452-3453 , denotes ,
T5127 3454-3458 NNP denotes Lake
T5128 3459-3465 NNP denotes Placid
T5129 3465-3466 , denotes ,
T5130 3467-3470 NNP denotes New
T5131 3471-3475 NNP denotes York
T5132 3475-3476 , denotes ,
T5133 3477-3480 NNP denotes USA
T5134 3480-3481 -RRB- denotes )
T5135 3481-3482 . denotes .
T5136 3483-3490 JJ denotes Western
T5137 3491-3495 NN denotes blot
T5138 3496-3504 NN denotes analysis
T5139 3505-3508 VBD denotes was
T5140 3509-3518 VBN denotes performed
T5141 3519-3524 VBG denotes using
T5142 3525-3536 JJ denotes anti-GATA-3
T5143 3536-3537 , denotes ,
T5144 3538-3553 JJ denotes anti-importin-α
T5145 3553-3554 , denotes ,
T5146 3555-3562 JJ denotes anti-GR
T5147 3562-3563 , denotes ,
T5148 3564-3574 JJ denotes anti-p-p38
T5149 3575-3578 NNP denotes MAP
T5150 3579-3585 NN denotes kinase
T5151 3585-3586 , denotes ,
T5152 3587-3599 JJ denotes anti-p-ATF-2
T5153 3599-3600 , denotes ,
T5154 3601-3604 CC denotes and
T5155 3605-3618 JJ denotes anti-p-serine
T5156 3618-3619 . denotes .
T5157 3620-3634 JJ denotes Immunoreactive
T5158 3635-3643 NNS denotes proteins
T5159 3644-3648 VBD denotes were
T5160 3649-3657 VBN denotes detected
T5161 3658-3663 VBG denotes using
T5162 3664-3666 DT denotes an
T5163 3667-3675 JJ denotes enhanced
T5164 3676-3693 NN denotes chemiluminescence
T5165 3694-3697 NNP denotes ECL
T5166 3698-3701 NN denotes kit
T5167 3702-3703 -LRB- denotes (
T5168 3703-3711 NNP denotes Amersham
T5169 3712-3723 NNPS denotes Biosciences
T5170 3723-3724 -RRB- denotes )
T5171 3724-3725 . denotes .
T5318 3727-3736 NNP denotes Chromatin
T5319 3737-3756 NNP denotes Immunoprecipitation
T5320 3757-3766 NNP denotes Chromatin
T5321 3767-3786 NN denotes immunoprecipitation
T5322 3787-3788 -LRB- denotes (
T5323 3788-3790 NNP denotes IP
T5324 3790-3791 -RRB- denotes )
T5325 3792-3795 VBD denotes was
T5326 3796-3805 VBN denotes performed
T5327 3806-3808 IN denotes as
T5328 3809-3819 RB denotes previously
T5329 3820-3829 VBN denotes described
T5330 3830-3831 NNP denotes [
T5331 3831-3833 CD denotes 12
T5332 3833-3834 NNP denotes ]
T5333 3835-3837 IN denotes in
T5334 3838-3844 NNP denotes HuT-78
T5335 3845-3852 NNS denotes T-cells
T5336 3853-3857 IN denotes with
T5337 3858-3859 CD denotes 2
T5338 3860-3862 NN denotes µg
T5339 3863-3865 IN denotes of
T5340 3866-3877 JJ denotes anti-GATA-3
T5341 3878-3879 -LRB- denotes (
T5342 3879-3884 NNP denotes Santa
T5343 3885-3889 NNP denotes Cruz
T5344 3890-3903 NNP denotes Biotechnology
T5345 3903-3904 -RRB- denotes )
T5346 3904-3905 , denotes ,
T5347 3906-3908 CC denotes or
T5348 3909-3917 JJ denotes isotypic
T5349 3918-3932 NN denotes immunoglobulin
T5350 3933-3934 NNP denotes G
T5351 3935-3937 IN denotes as
T5352 3938-3939 DT denotes a
T5353 3940-3952 JJ denotes non-specific
T5354 3953-3960 NN denotes control
T5355 3961-3962 -LRB- denotes (
T5356 3962-3967 NNP denotes Santa
T5357 3968-3972 NNP denotes Cruz
T5358 3973-3986 NNP denotes Biotechnology
T5359 3986-3987 -RRB- denotes )
T5360 3988-3997 RB denotes overnight
T5361 3998-4000 IN denotes at
T5362 4001-4002 CD denotes 4
T5363 4002-4003 CD denotes °
T5364 4003-4005 NNP denotes C.
T5365 4006-4014 NNP denotes Promoter
T5366 4015-4024 NNS denotes sequences
T5367 4025-4029 VBD denotes were
T5368 4030-4038 VBN denotes detected
T5369 4039-4043 IN denotes with
T5370 4044-4047 NNP denotes PCR
T5371 4048-4055 NNS denotes primers
T5372 4056-4059 IN denotes for
T5373 4060-4063 DT denotes the
T5374 4064-4068 NN denotes IL-5
T5375 4069-4077 NN denotes promoter
T5376 4078-4079 -LRB- denotes (
T5377 4079-4080 FW denotes −
T5378 4080-4083 CD denotes 445
T5379 4084-4086 TO denotes to
T5380 4087-4089 CD denotes +4
T5381 4089-4090 -RRB- denotes )
T5382 4090-4091 : denotes :
T5383 4092-4099 RB denotes forward
T5384 4100-4101 CD denotes 5
T5385 4101-4102 SYM denotes ′
T5386 4102-4103 : denotes -
T5387 4103-4126 NN denotes TTAATCTAGCCACAGTCATAG-3
T5388 4126-4127 NN denotes ′
T5389 4128-4131 CC denotes and
T5390 4132-4139 NN denotes reverse
T5391 4139-4140 : denotes :
T5392 4141-4142 CD denotes 5
T5393 4142-4143 SYM denotes ′
T5394 4143-4144 : denotes -
T5395 4144-4167 NN denotes TCATGGCTCTGAAACGTTCTG-3
T5396 4167-4168 NN denotes ′
T5397 4168-4169 . denotes .
T5398 4170-4173 NNP denotes PCR
T5399 4174-4177 VBD denotes was
T5400 4178-4187 VBN denotes performed
T5401 4188-4193 VBG denotes using
T5402 4194-4195 DT denotes a
T5403 4196-4202 NNP denotes Hybaid
T5404 4203-4211 NNP denotes Omnigene
T5405 4212-4219 JJ denotes thermal
T5406 4220-4226 NN denotes cycler
T5407 4227-4228 -LRB- denotes (
T5408 4228-4234 NNP denotes Hybaid
T5409 4234-4235 , denotes ,
T5410 4236-4243 NNP denotes Ashford
T5411 4243-4244 , denotes ,
T5412 4245-4247 NNP denotes UK
T5413 4247-4248 -RRB- denotes )
T5414 4249-4253 IN denotes with
T5415 4254-4261 NN denotes cycling
T5416 4262-4272 NNS denotes parameters
T5417 4273-4275 IN denotes of
T5418 4276-4278 CD denotes 72
T5419 4278-4279 CD denotes °
T5420 4279-4280 NNP denotes C
T5421 4281-4284 IN denotes for
T5422 4285-4287 CD denotes 10
T5423 4288-4291 NN denotes min
T5424 4291-4292 , denotes ,
T5425 4293-4295 CD denotes 35
T5426 4296-4302 NNS denotes cycles
T5427 4303-4305 IN denotes at
T5428 4306-4308 CD denotes 94
T5429 4308-4309 CD denotes °
T5430 4309-4310 NNP denotes C
T5431 4311-4314 IN denotes for
T5432 4315-4317 CD denotes 45
T5433 4318-4319 PRP denotes s
T5434 4319-4320 , denotes ,
T5435 4321-4323 CD denotes 52
T5436 4323-4324 NN denotes °
T5437 4324-4325 NNP denotes C
T5438 4326-4329 IN denotes for
T5439 4330-4332 CD denotes 45
T5440 4333-4334 PRP denotes s
T5441 4334-4335 , denotes ,
T5442 4336-4338 CD denotes 72
T5443 4338-4339 NN denotes °
T5444 4339-4340 NNP denotes C
T5445 4341-4344 IN denotes for
T5446 4345-4347 CD denotes 45
T5447 4348-4350 NN denotes s.
T5754 4352-4361 CD denotes GR-GATA-3
T5755 4362-4364 IN denotes In
T5756 4365-4370 NNP denotes Vitro
T5757 4371-4382 NNP denotes Competition
T5758 4383-4388 NNP denotes Assay
T5759 4389-4392 IN denotes for
T5760 4393-4403 NNP denotes Importin-α
T5761 4404-4405 -LRB- denotes (
T5762 4405-4416 NNP denotes Far-Western
T5763 4417-4422 NNP denotes ELISA
T5764 4422-4423 -RRB- denotes )
T5765 4424-4442 NNP denotes Immunoprecipitated
T5766 4443-4453 NN denotes importin-α
T5767 4454-4455 -LRB- denotes (
T5768 4455-4470 JJ denotes anti-importin-α
T5769 4470-4471 , denotes ,
T5770 4472-4477 NNP denotes Santa
T5771 4478-4482 NNP denotes Cruz
T5772 4483-4496 NNP denotes Biotechnology
T5773 4496-4497 -RRB- denotes )
T5774 4498-4502 IN denotes from
T5775 4503-4509 JJ denotes HuT-78
T5776 4510-4515 NNS denotes cells
T5777 4516-4519 VBD denotes was
T5778 4520-4529 VBN denotes separated
T5779 4530-4532 IN denotes by
T5780 4533-4541 NNP denotes SDS-PAGE
T5781 4542-4545 CC denotes and
T5782 4546-4554 VBN denotes purified
T5783 4555-4559 IN denotes from
T5784 4560-4563 DT denotes the
T5785 4564-4571 VBN denotes excised
T5786 4572-4575 NN denotes gel
T5787 4576-4578 IN denotes by
T5788 4579-4593 NN denotes electroelution
T5789 4594-4595 NNP denotes [
T5790 4595-4597 CD denotes 36
T5791 4597-4598 NNP denotes ]
T5792 4598-4599 . denotes .
T5793 4600-4609 RB denotes Similarly
T5794 4609-4610 , denotes ,
T5795 4611-4617 NN denotes GATA-3
T5796 4618-4621 VBD denotes was
T5797 4622-4630 VBN denotes isolated
T5798 4631-4635 IN denotes from
T5799 4636-4649 JJ denotes nonstimulated
T5800 4650-4656 JJ denotes HuT-78
T5801 4657-4662 NNS denotes cells
T5802 4663-4665 CC denotes or
T5803 4666-4671 NNS denotes cells
T5804 4672-4682 VBD denotes stimulated
T5805 4683-4686 IN denotes for
T5806 4687-4689 CD denotes 30
T5807 4690-4693 NN denotes min
T5808 4694-4698 IN denotes with
T5809 4699-4707 JJ denotes anti-CD3
T5810 4707-4708 NN denotes /
T5811 4708-4712 CD denotes CD28
T5812 4713-4716 CC denotes and
T5813 4717-4719 NNP denotes GR
T5814 4720-4723 VBD denotes was
T5815 4724-4732 VBN denotes isolated
T5816 4733-4737 IN denotes from
T5817 4738-4740 NNP denotes FP
T5818 4741-4742 -LRB- denotes (
T5819 4742-4744 CD denotes 10
T5820 4744-4745 NN denotes −
T5821 4745-4746 CD denotes 8
T5822 4747-4748 NNP denotes M
T5823 4748-4749 , denotes ,
T5824 4750-4752 CD denotes 30
T5825 4753-4756 NN denotes min
T5826 4756-4757 -RRB- denotes )
T5827 4758-4768 VBD denotes stimulated
T5828 4769-4775 JJ denotes HuT-78
T5829 4776-4781 NNS denotes cells
T5830 4781-4782 . denotes .
T5831 4783-4788 DT denotes These
T5832 4789-4797 NNS denotes proteins
T5833 4798-4802 VBD denotes were
T5834 4803-4815 RB denotes subsequently
T5835 4816-4824 VBN denotes refolded
T5836 4825-4827 IN denotes in
T5837 4828-4835 NN denotes glycine
T5838 4836-4844 NN denotes solution
T5839 4844-4845 . denotes .
T5840 4846-4849 CD denotes 100
T5841 4850-4852 NN denotes µl
T5842 4853-4855 IN denotes of
T5843 4856-4866 JJ denotes importin-α
T5844 4867-4875 NN denotes solution
T5845 4876-4877 -LRB- denotes (
T5846 4877-4880 CD denotes 100
T5847 4881-4886 NN denotes ng/ml
T5848 4887-4889 IN denotes in
T5849 4890-4893 NNP denotes TBS
T5850 4893-4894 -RRB- denotes )
T5851 4895-4898 VBD denotes was
T5852 4899-4904 VBN denotes added
T5853 4905-4907 TO denotes to
T5854 4908-4915 JJ denotes 96-well
T5855 4916-4922 NNS denotes plates
T5856 4923-4929 VBN denotes coated
T5857 4930-4934 IN denotes with
T5858 4935-4939 NN denotes goat
T5859 4940-4955 JJ denotes anti-importin-α
T5860 4956-4964 NN denotes antibody
T5861 4964-4965 . denotes .
T5862 4966-4971 IN denotes After
T5863 4972-4973 CD denotes 1
T5864 4974-4975 NN denotes h
T5865 4976-4986 NN denotes incubation
T5866 4986-4987 , denotes ,
T5867 4988-4994 NNP denotes GATA-3
T5868 4995-4996 -LRB- denotes (
T5869 4996-4999 CD denotes 100
T5870 5000-5005 NN denotes ng/ml
T5871 5006-5008 IN denotes in
T5872 5009-5012 NNP denotes TBS
T5873 5012-5013 -RRB- denotes )
T5874 5014-5018 IN denotes from
T5875 5019-5029 VBN denotes stimulated
T5876 5030-5032 CC denotes or
T5877 5033-5045 JJ denotes unstimulated
T5878 5046-5051 NNS denotes cells
T5879 5052-5056 VBD denotes were
T5880 5057-5062 VBN denotes added
T5881 5063-5065 TO denotes to
T5882 5066-5069 DT denotes the
T5883 5070-5087 JJ denotes importin-α-coated
T5884 5088-5093 NNS denotes wells
T5885 5093-5094 . denotes .
T5886 5095-5097 NNP denotes GR
T5887 5098-5099 -LRB- denotes (
T5888 5099-5101 CD denotes 10
T5889 5102-5104 CC denotes or
T5890 5105-5108 CD denotes 100
T5891 5109-5114 NN denotes ng/ml
T5892 5115-5117 IN denotes in
T5893 5118-5121 NNP denotes TBS
T5894 5121-5122 -RRB- denotes )
T5895 5123-5127 IN denotes from
T5896 5128-5138 VBN denotes stimulated
T5897 5139-5141 CC denotes or
T5898 5142-5154 JJ denotes unstimulated
T5899 5155-5160 NNS denotes cells
T5900 5161-5165 VBD denotes were
T5901 5166-5171 VBN denotes added
T5902 5172-5174 TO denotes to
T5903 5175-5179 DT denotes some
T5904 5180-5185 NNS denotes wells
T5905 5185-5186 . denotes .
T5906 5187-5192 IN denotes After
T5907 5193-5194 DT denotes a
T5908 5195-5202 JJ denotes further
T5909 5203-5204 CD denotes 1
T5910 5205-5206 NN denotes h
T5911 5207-5217 NN denotes incubation
T5912 5217-5218 , denotes ,
T5913 5219-5222 DT denotes the
T5914 5223-5228 NN denotes plate
T5915 5229-5232 VBD denotes was
T5916 5233-5239 VBN denotes washed
T5917 5240-5243 CC denotes and
T5918 5244-5253 VBN denotes incubated
T5919 5254-5258 IN denotes with
T5920 5259-5266 JJ denotes primary
T5921 5267-5277 NNS denotes antibodies
T5922 5278-5279 -LRB- denotes (
T5923 5279-5280 DT denotes a
T5924 5281-5288 NN denotes mixture
T5925 5289-5291 IN denotes of
T5926 5292-5298 NN denotes rabbit
T5927 5299-5310 JJ denotes anti-GATA-3
T5928 5311-5314 CC denotes and
T5929 5315-5320 NN denotes mouse
T5930 5321-5328 JJ denotes anti-GR
T5931 5328-5329 , denotes ,
T5932 5330-5335 NNP denotes Santa
T5933 5336-5340 NNP denotes Cruz
T5934 5341-5354 NNP denotes Biotechnology
T5935 5354-5355 -RRB- denotes )
T5936 5356-5359 IN denotes for
T5937 5360-5363 CD denotes 1.5
T5938 5364-5365 NN denotes h
T5939 5365-5366 , denotes ,
T5940 5367-5370 CC denotes and
T5941 5371-5375 RB denotes then
T5942 5376-5385 VBN denotes incubated
T5943 5386-5390 IN denotes with
T5944 5391-5400 JJ denotes secondary
T5945 5401-5411 NNS denotes antibodies
T5946 5411-5412 . denotes .
T5947 5413-5417 CD denotes FITC
T5948 5418-5423 NNS denotes swine
T5949 5424-5435 JJ denotes anti-rabbit
T5950 5436-5439 NNP denotes IgG
T5951 5440-5441 -LRB- denotes (
T5952 5441-5445 NNP denotes Dako
T5953 5445-5446 , denotes ,
T5954 5447-5456 NNP denotes Cambridge
T5955 5456-5457 , denotes ,
T5956 5458-5460 NNP denotes UK
T5957 5460-5461 -RRB- denotes )
T5958 5462-5465 VBD denotes was
T5959 5466-5470 VBN denotes used
T5960 5471-5474 IN denotes for
T5961 5475-5478 DT denotes the
T5962 5479-5488 NN denotes detection
T5963 5489-5491 IN denotes of
T5964 5492-5498 NNP denotes GATA-3
T5965 5498-5499 , denotes ,
T5966 5500-5503 CC denotes and
T5967 5504-5513 JJ denotes rhodamine
T5968 5514-5520 NN denotes donkey
T5969 5521-5531 JJ denotes anti-mouse
T5970 5532-5535 NNP denotes IgG
T5971 5536-5537 -LRB- denotes (
T5972 5537-5542 NNP denotes Novus
T5973 5542-5543 , denotes ,
T5974 5544-5553 NNP denotes Littleton
T5975 5553-5554 , denotes ,
T5976 5555-5563 NNP denotes Colorado
T5977 5563-5564 , denotes ,
T5978 5565-5568 NNP denotes USA
T5979 5568-5569 -RRB- denotes )
T5980 5570-5573 VBD denotes was
T5981 5574-5578 VBN denotes used
T5982 5579-5582 IN denotes for
T5983 5583-5586 DT denotes the
T5984 5587-5596 NN denotes detection
T5985 5597-5599 IN denotes of
T5986 5600-5602 NNP denotes GR
T5987 5602-5603 . denotes .
T5988 5604-5608 NNP denotes FITC
T5989 5609-5612 CC denotes and
T5990 5613-5622 JJ denotes rhodamine
T5991 5623-5629 NNS denotes levels
T5992 5630-5634 VBD denotes were
T5993 5635-5643 VBN denotes measured
T5994 5644-5648 IN denotes with
T5995 5649-5650 DT denotes a
T5996 5651-5662 JJ denotes fluorescent
T5997 5663-5674 JJ denotes micro-plate
T5998 5675-5681 NN denotes reader
T5999 5682-5683 -LRB- denotes (
T6000 5683-5690 NNP denotes Bioline
T6001 5690-5691 , denotes ,
T6002 5692-5698 NNP denotes London
T6003 5698-5699 , denotes ,
T6004 5700-5702 NNP denotes UK
T6005 5702-5703 -RRB- denotes )
T6006 5703-5704 . denotes .
T6161 5706-5711 NNP denotes NF-κB
T6162 5712-5722 NNP denotes Activation
T6163 5723-5728 NNP denotes NF-κB
T6164 5729-5736 JJ denotes binding
T6165 5737-5745 NN denotes activity
T6166 5746-5748 IN denotes in
T6167 5749-5756 JJ denotes nuclear
T6168 5757-5765 NNS denotes extracts
T6169 5766-5769 VBD denotes was
T6170 5770-5780 VBN denotes determined
T6171 5781-5786 VBG denotes using
T6172 5787-5789 DT denotes an
T6173 5790-5801 JJ denotes ELISA-based
T6174 5802-5805 NN denotes kit
T6175 5806-5807 -LRB- denotes (
T6176 5807-5815 JJ denotes Trans-AM
T6177 5816-5819 NNS denotes p65
T6178 5819-5820 , denotes ,
T6179 5821-5827 NNP denotes Active
T6180 5828-5833 NNP denotes Motif
T6181 5833-5834 , denotes ,
T6182 5835-5844 NNP denotes Rixensart
T6183 5844-5845 , denotes ,
T6184 5846-5853 NNP denotes Belgium
T6185 5853-5854 -RRB- denotes )
T6186 5854-5855 . denotes .
T6187 5856-5858 IN denotes In
T6188 5859-5864 NN denotes brief
T6189 5864-5865 , denotes ,
T6190 5866-5867 CD denotes 5
T6191 5868-5870 NN denotes µg
T6192 5871-5873 IN denotes of
T6193 5874-5881 JJ denotes nuclear
T6194 5882-5890 NNS denotes extracts
T6195 5891-5895 VBD denotes were
T6196 5896-5905 VBN denotes incubated
T6197 5906-5910 IN denotes with
T6198 5911-5912 DT denotes a
T6199 5913-5918 NN denotes plate
T6200 5919-5925 VBN denotes coated
T6201 5926-5930 IN denotes with
T6202 5931-5933 DT denotes an
T6203 5934-5939 JJ denotes NF-κB
T6204 5940-5949 NN denotes consensus
T6205 5950-5965 NN denotes oligonucleotide
T6206 5965-5966 . denotes .
T6207 5967-5973 NNS denotes Plates
T6208 5974-5978 VBD denotes were
T6209 5979-5985 VBN denotes washed
T6210 5986-5992 IN denotes before
T6211 5993-6001 NN denotes addition
T6212 6002-6004 IN denotes of
T6213 6005-6007 DT denotes an
T6214 6008-6016 JJ denotes anti-p65
T6215 6017-6025 NN denotes antibody
T6216 6025-6026 . denotes .
T6217 6027-6035 NN denotes Antibody
T6218 6036-6043 JJ denotes binding
T6219 6044-6047 VBD denotes was
T6220 6048-6056 VBN denotes detected
T6221 6057-6061 IN denotes with
T6222 6062-6063 DT denotes a
T6223 6064-6073 JJ denotes secondary
T6224 6074-6088 JJ denotes HRP-conjugated
T6225 6089-6097 NN denotes antibody
T6226 6098-6101 CC denotes and
T6227 6102-6111 VBN denotes developed
T6228 6112-6116 IN denotes with
T6229 6117-6120 NNP denotes TMB
T6230 6121-6130 NN denotes substrate
T6231 6130-6131 . denotes .
T6232 6132-6135 DT denotes The
T6233 6136-6145 NN denotes intensity
T6234 6146-6148 IN denotes of
T6235 6149-6152 DT denotes the
T6236 6153-6161 NN denotes reaction
T6237 6162-6165 VBD denotes was
T6238 6166-6174 VBN denotes measured
T6239 6175-6177 IN denotes at
T6240 6178-6181 CD denotes 450
T6241 6182-6184 NN denotes nm
T6242 6184-6185 . denotes .
T6486 6187-6205 NNP denotes Immunofluorescence
T6487 6206-6214 NNP denotes Staining
T6488 6215-6233 NNP denotes Immunofluorescence
T6489 6234-6242 VBG denotes staining
T6490 6243-6246 VBD denotes was
T6491 6247-6256 VBN denotes performed
T6492 6257-6259 IN denotes as
T6493 6260-6270 RB denotes previously
T6494 6271-6280 VBN denotes described
T6495 6281-6282 NNP denotes [
T6496 6282-6284 CD denotes 34
T6497 6284-6285 NNP denotes ]
T6498 6285-6286 . denotes .
T6499 6287-6290 DT denotes All
T6500 6291-6299 VBG denotes staining
T6501 6300-6303 VBD denotes was
T6502 6304-6313 VBN denotes performed
T6503 6314-6316 IN denotes at
T6504 6317-6321 NN denotes room
T6505 6322-6333 NN denotes temperature
T6506 6334-6337 CC denotes and
T6507 6338-6343 IN denotes under
T6508 6344-6358 NN denotes humidification
T6509 6358-6359 . denotes .
T6510 6360-6365 NNS denotes Cells
T6511 6366-6370 VBD denotes were
T6512 6371-6380 VBN denotes collected
T6513 6381-6384 CC denotes and
T6514 6385-6394 NNS denotes cytospins
T6515 6395-6403 VBN denotes prepared
T6516 6404-6406 IN denotes in
T6517 6407-6408 DT denotes a
T6518 6409-6423 NN denotes cytocentrifuge
T6519 6424-6425 -LRB- denotes (
T6520 6425-6432 NNP denotes Shandon
T6521 6433-6435 NNP denotes II
T6522 6435-6436 , denotes ,
T6523 6437-6444 NNP denotes Shandon
T6524 6444-6445 , denotes ,
T6525 6446-6453 NNP denotes Runcorn
T6526 6453-6454 , denotes ,
T6527 6455-6457 NNP denotes UK
T6528 6457-6458 -RRB- denotes )
T6529 6458-6459 . denotes .
T6530 6460-6465 NNS denotes Cells
T6531 6466-6470 VBD denotes were
T6532 6471-6484 VBN denotes permeabilized
T6533 6484-6485 , denotes ,
T6534 6486-6493 VBN denotes blocked
T6535 6493-6494 , denotes ,
T6536 6495-6498 CC denotes and
T6537 6499-6503 RB denotes then
T6538 6504-6513 VBN denotes incubated
T6539 6514-6518 IN denotes with
T6540 6519-6521 NNP denotes GR
T6541 6522-6523 -LRB- denotes (
T6542 6523-6524 CD denotes 1
T6543 6524-6525 CD denotes ∶
T6544 6525-6527 CD denotes 50
T6545 6528-6532 NN denotes E-20
T6546 6533-6538 NNP denotes Santa
T6547 6539-6543 NNP denotes Cruz
T6548 6544-6557 NNP denotes Biotechnology
T6549 6557-6558 -RRB- denotes )
T6550 6559-6561 CC denotes or
T6551 6562-6573 JJ denotes anti-GATA-3
T6552 6574-6575 -LRB- denotes (
T6553 6575-6579 JJ denotes H-48
T6554 6579-6580 : denotes ;
T6555 6581-6586 NNP denotes Santa
T6556 6587-6591 NNP denotes Cruz
T6557 6592-6605 NNP denotes Biotechnology
T6558 6605-6606 -RRB- denotes )
T6559 6607-6615 NN denotes antibody
T6560 6616-6619 IN denotes for
T6561 6620-6621 CD denotes 1
T6562 6622-6623 NN denotes h
T6563 6624-6626 IN denotes at
T6564 6627-6631 NN denotes room
T6565 6632-6643 NN denotes temperature
T6566 6643-6644 . denotes .
T6567 6645-6650 IN denotes After
T6568 6651-6656 CD denotes three
T6569 6657-6663 NNS denotes washes
T6570 6664-6666 IN denotes in
T6571 6667-6685 JJ denotes phosphate-buffered
T6572 6686-6692 NN denotes saline
T6573 6693-6694 -LRB- denotes (
T6574 6694-6697 NNP denotes PBS
T6575 6697-6698 -RRB- denotes )
T6576 6698-6699 , denotes ,
T6577 6700-6705 NNS denotes cells
T6578 6706-6710 VBD denotes were
T6579 6711-6720 VBN denotes incubated
T6580 6721-6725 IN denotes with
T6581 6726-6740 JJ denotes tetrarhodamine
T6582 6741-6755 NN denotes isothiocyanate
T6583 6756-6766 VBN denotes conjugated
T6584 6767-6771 NN denotes goat
T6585 6772-6783 JJ denotes anti-rabbit
T6586 6784-6792 NN denotes antibody
T6587 6793-6794 -LRB- denotes (
T6588 6794-6798 NNP denotes Dako
T6589 6798-6799 -RRB- denotes )
T6590 6799-6800 . denotes .
T6591 6801-6810 NNS denotes Cytospins
T6592 6811-6815 VBD denotes were
T6593 6816-6830 VBN denotes counterstained
T6594 6831-6835 IN denotes with
T6595 6836-6837 CD denotes 4
T6596 6837-6838 CD denotes ′
T6597 6838-6840 SYM denotes ,6
T6598 6840-6841 : denotes -
T6599 6841-6865 JJ denotes diamidino-2-phenylindole
T6600 6866-6881 NN denotes dihydrochloride
T6601 6882-6883 -LRB- denotes (
T6602 6883-6887 NNP denotes DAPI
T6603 6887-6888 -RRB- denotes )
T6604 6888-6889 , denotes ,
T6605 6890-6891 DT denotes a
T6606 6892-6903 JJ denotes fluorescent
T6607 6904-6908 JJ denotes blue
T6608 6909-6916 JJ denotes nuclear
T6609 6917-6923 NN denotes indole
T6610 6924-6933 NN denotes chromatin
T6611 6934-6939 VBP denotes stain
T6612 6939-6940 , denotes ,
T6613 6941-6944 CC denotes and
T6614 6945-6952 VBD denotes mounted
T6615 6953-6955 IN denotes in
T6616 6956-6959 NNP denotes PBS
T6617 6959-6960 : denotes :
T6618 6960-6968 NN denotes glycerol
T6619 6969-6970 -LRB- denotes (
T6620 6970-6972 CD denotes 50
T6621 6972-6973 NN denotes ∶
T6622 6973-6975 CD denotes 50
T6623 6975-6976 -RRB- denotes )
T6624 6976-6977 . denotes .
T6625 6978-6981 DT denotes The
T6626 6982-6996 JJ denotes immunopositive
T6627 6997-7003 NN denotes signal
T6628 7004-7007 VBD denotes was
T6629 7008-7021 VBN denotes characterised
T6630 7022-7027 VBG denotes using
T6631 7028-7033 NN denotes laser
T6632 7034-7042 VBG denotes scanning
T6633 7043-7051 JJ denotes confocal
T6634 7052-7062 NN denotes microscopy
T6635 7063-7065 IN denotes on
T6636 7066-7067 DT denotes a
T6637 7068-7073 NNP denotes Leica
T6638 7074-7077 NNP denotes TCS
T6639 7078-7083 NNP denotes NT/SP
T6640 7084-7095 JJ denotes interactive
T6641 7096-7101 NN denotes laser
T6642 7102-7111 NN denotes cytometer
T6643 7112-7120 VBN denotes equipped
T6644 7121-7125 IN denotes with
T6645 7126-7134 JJ denotes confocal
T6646 7135-7141 NNS denotes optics
T6647 7142-7143 -LRB- denotes (
T6648 7143-7148 NNP denotes Leica
T6649 7149-7161 NNP denotes Microsystems
T6650 7161-7162 , denotes ,
T6651 7163-7170 NNP denotes Wetzlar
T6652 7170-7171 , denotes ,
T6653 7172-7179 NNP denotes Germany
T6654 7179-7180 -RRB- denotes )
T6655 7180-7181 . denotes .
T6656 7182-7184 TO denotes To
T6657 7185-7194 VB denotes determine
T6658 7195-7198 DT denotes the
T6659 7199-7210 NN denotes specificity
T6660 7211-7213 IN denotes of
T6661 7214-7217 DT denotes the
T6662 7218-7228 NNS denotes antibodies
T6663 7228-7229 , denotes ,
T6664 7230-7236 NN denotes rabbit
T6665 7237-7242 NN denotes serum
T6666 7243-7257 NN denotes immunoglobulin
T6667 7258-7259 -LRB- denotes (
T6668 7259-7263 NNP denotes Dako
T6669 7263-7264 -RRB- denotes )
T6670 7265-7268 CC denotes and
T6671 7269-7278 JJ denotes secondary
T6672 7279-7289 NNS denotes antibodies
T6673 7290-7297 IN denotes without
T6674 7298-7301 DT denotes the
T6675 7302-7309 JJ denotes primary
T6676 7310-7314 VBD denotes were
T6677 7315-7319 VBN denotes used
T6678 7320-7322 IN denotes as
T6679 7323-7331 NNS denotes controls
T6680 7331-7332 . denotes .
T6681 7333-7343 RB denotes Positively
T6682 7344-7351 VBN denotes stained
T6683 7352-7358 NNS denotes nuclei
T6684 7359-7362 CC denotes and
T6685 7363-7368 JJ denotes total
T6686 7369-7374 NNS denotes cells
T6687 7375-7379 VBD denotes were
T6688 7380-7387 VBN denotes counted
T6689 7388-7389 -LRB- denotes (
T6690 7389-7392 CD denotes 500
T6691 7392-7393 -RRB- denotes )
T6692 7394-7396 IN denotes on
T6693 7397-7401 DT denotes each
T6694 7402-7407 NN denotes slide
T6695 7408-7412 IN denotes with
T6696 7413-7416 DT denotes the
T6697 7417-7425 NN denotes observer
T6698 7426-7433 VBD denotes blinded
T6699 7434-7436 TO denotes to
T6700 7437-7440 DT denotes the
T6701 7441-7450 NN denotes treatment
T6702 7450-7451 . denotes .
T6937 7453-7463 NNP denotes GATA-3-GFP
T6938 7464-7473 NNP denotes Construct
T6939 7474-7477 NNP denotes The
T6940 7478-7484 NNP denotes GATA-3
T6941 7485-7490 NN denotes clone
T6942 7491-7492 -LRB- denotes (
T6943 7492-7500 NNP denotes BC003070
T6944 7500-7501 -RRB- denotes )
T6945 7502-7510 JJ denotes complete
T6946 7511-7515 NNP denotes cDNA
T6947 7516-7519 VBD denotes was
T6948 7520-7528 VBN denotes obtained
T6949 7529-7533 IN denotes from
T6950 7534-7544 NNP denotes Invitrogen
T6951 7545-7549 NNP denotes Life
T6952 7550-7562 NNPS denotes Technologies
T6953 7563-7565 IN denotes as
T6954 7566-7567 DT denotes a
T6955 7568-7569 CD denotes 5
T6956 7569-7570 SYM denotes ′
T6957 7570-7571 : denotes -
T6958 7572-7579 JJ denotes EcoRI/3
T6959 7579-7580 SYM denotes ′
T6960 7580-7581 : denotes -
T6961 7581-7585 NNP denotes XhoI
T6962 7586-7592 NN denotes insert
T6963 7593-7595 IN denotes of
T6964 7596-7602 NN denotes GATA-3
T6965 7603-7605 IN denotes in
T6966 7606-7609 DT denotes the
T6967 7610-7615 NNP denotes pOTB7
T6968 7616-7622 NN denotes vector
T6969 7622-7623 . denotes .
T6970 7624-7630 NN denotes GATA-3
T6971 7631-7634 VBD denotes was
T6972 7635-7642 VBN denotes excised
T6973 7643-7647 IN denotes from
T6974 7648-7653 NNP denotes pOTB7
T6975 7654-7659 VBG denotes using
T6976 7660-7664 NNP denotes XhoI
T6977 7665-7674 NN denotes digestion
T6978 7675-7678 CC denotes and
T6979 7679-7687 NN denotes pEGFP-C2
T6980 7688-7689 -LRB- denotes (
T6981 7689-7697 NNP denotes Clontech
T6982 7697-7698 , denotes ,
T6983 7699-7720 NNP denotes Saint-Germain-en-Laye
T6984 7720-7721 , denotes ,
T6985 7722-7728 NNP denotes France
T6986 7728-7729 -RRB- denotes )
T6987 7730-7733 VBD denotes was
T6988 7734-7742 VBN denotes digested
T6989 7743-7747 IN denotes with
T6990 7748-7753 NNP denotes BamHI
T6991 7753-7754 . denotes .
T6992 7755-7758 NNP denotes DNA
T6993 7759-7762 VBD denotes was
T6994 7763-7772 VBN denotes recovered
T6995 7773-7775 IN denotes by
T6996 7776-7782 NN denotes phenol
T6997 7783-7793 NN denotes extraction
T6998 7794-7797 CC denotes and
T6999 7798-7805 NN denotes ethanol
T7000 7806-7819 NN denotes precipitation
T7001 7819-7820 , denotes ,
T7002 7821-7824 CC denotes and
T7003 7825-7829 DT denotes both
T7004 7830-7833 DT denotes the
T7005 7834-7840 NNP denotes GATA-3
T7006 7841-7849 NN denotes fragment
T7007 7850-7853 CC denotes and
T7008 7854-7857 DT denotes the
T7009 7858-7866 NN denotes pEGFP-C2
T7010 7867-7873 NN denotes vector
T7011 7874-7885 JJ denotes blunt-ended
T7012 7886-7888 IN denotes by
T7013 7889-7899 NN denotes incubation
T7014 7900-7904 IN denotes with
T7015 7905-7911 NNP denotes Klenow
T7016 7912-7913 -LRB- denotes (
T7017 7913-7920 NNP denotes Bioline
T7018 7921-7930 NNP denotes Bio-27029
T7019 7930-7931 -RRB- denotes )
T7020 7932-7935 IN denotes for
T7021 7936-7938 CD denotes 30
T7022 7939-7942 NN denotes min
T7023 7943-7945 IN denotes at
T7024 7946-7948 CD denotes 37
T7025 7948-7949 CD denotes °
T7026 7949-7951 NNP denotes C.
T7027 7952-7958 NNP denotes Klenow
T7028 7959-7962 VBD denotes was
T7029 7963-7974 VBN denotes inactivated
T7030 7975-7977 IN denotes by
T7031 7978-7988 NN denotes incubation
T7032 7989-7992 IN denotes for
T7033 7993-7995 CD denotes 10
T7034 7996-7999 NN denotes min
T7035 8000-8002 IN denotes at
T7036 8003-8005 CD denotes 75
T7037 8005-8006 CD denotes °
T7038 8006-8008 NNP denotes C.
T7039 8009-8012 NNP denotes DNA
T7040 8013-8016 VBD denotes was
T7041 8017-8026 VBN denotes recovered
T7042 8027-8029 IN denotes by
T7043 8030-8036 NN denotes phenol
T7044 8037-8047 NN denotes extraction
T7045 8048-8051 CC denotes and
T7046 8052-8059 NN denotes ethanol
T7047 8060-8073 NN denotes precipitation
T7048 8073-8074 , denotes ,
T7049 8075-8078 CC denotes and
T7050 8079-8083 DT denotes both
T7051 8084-8087 DT denotes the
T7052 8088-8099 JJ denotes blunt-ended
T7053 8100-8106 JJ denotes GATA-3
T7054 8107-8115 NN denotes fragment
T7055 8116-8119 CC denotes and
T7056 8120-8123 DT denotes the
T7057 8124-8127 NNP denotes GFP
T7058 8128-8134 NN denotes vector
T7059 8135-8139 VBD denotes were
T7060 8140-8152 RB denotes subsequently
T7061 8153-8161 VBN denotes digested
T7062 8162-8166 IN denotes with
T7063 8167-8172 NNP denotes EcoRI
T7064 8173-8179 IN denotes before
T7065 8180-8183 DT denotes the
T7066 8184-8185 CD denotes 5
T7067 8185-8186 SYM denotes ′
T7068 8186-8187 : denotes -
T7069 8187-8194 JJ denotes EcoRI/3
T7070 8194-8195 NN denotes ′
T7071 8196-8201 JJ denotes blunt
T7072 8202-8205 NN denotes end
T7073 8206-8212 NN denotes GATA-3
T7074 8213-8221 NN denotes fragment
T7075 8222-8225 VBD denotes was
T7076 8226-8234 VBN denotes inserted
T7077 8235-8239 IN denotes into
T7078 8240-8243 DT denotes the
T7079 8244-8245 CD denotes 5
T7080 8245-8246 SYM denotes ′
T7081 8246-8247 : denotes -
T7082 8247-8254 JJ denotes EcoRI/3
T7083 8254-8255 NN denotes ′
T7084 8256-8261 JJ denotes blunt
T7085 8262-8267 VBN denotes ended
T7086 8268-8271 NNP denotes GFP
T7087 8272-8278 NN denotes vector
T7088 8278-8279 . denotes .
T7089 8280-8288 JJ denotes Positive
T7090 8289-8295 NNS denotes clones
T7091 8296-8300 VBD denotes were
T7092 8301-8310 VBN denotes confirmed
T7093 8311-8313 IN denotes by
T7094 8314-8323 NN denotes digestion
T7095 8324-8327 CC denotes and
T7096 8328-8332 NN denotes size
T7097 8333-8341 NN denotes analysis
T7098 8342-8344 IN denotes by
T7099 8345-8346 CD denotes 1
T7100 8346-8347 NN denotes %
T7101 8348-8355 JJ denotes agarose
T7102 8356-8359 NN denotes gel
T7103 8360-8375 NN denotes electrophoresis
T7104 8376-8379 CC denotes and
T7105 8380-8382 IN denotes by
T7106 8383-8393 VBG denotes sequencing
T7107 8393-8394 . denotes .
T7254 8396-8408 NNP denotes Transfection
T7255 8409-8415 NNP denotes HuT-78
T7256 8416-8421 NNS denotes cells
T7257 8422-8426 VBD denotes were
T7258 8427-8438 VBN denotes transfected
T7259 8439-8443 IN denotes with
T7260 8444-8450 DT denotes either
T7261 8451-8454 CD denotes EP8
T7262 8455-8457 CC denotes or
T7263 8458-8461 NNP denotes GFP
T7264 8462-8468 NN denotes vector
T7265 8469-8473 RB denotes only
T7266 8474-8477 NNP denotes DNA
T7267 8478-8483 VBG denotes using
T7268 8484-8492 NN denotes solution
T7269 8493-8494 NN denotes R
T7270 8494-8495 , denotes ,
T7271 8496-8505 NN denotes programme
T7272 8506-8511 NN denotes V-001
T7273 8512-8514 IN denotes at
T7274 8515-8516 DT denotes a
T7275 8517-8522 NN denotes ratio
T7276 8523-8525 IN denotes of
T7277 8526-8527 CD denotes 3
T7278 8527-8528 CD denotes ×
T7279 8528-8531 CD denotes 106
T7280 8532-8539 CD denotes cells/4
T7281 8540-8542 NN denotes µg
T7282 8543-8546 NN denotes DNA
T7283 8547-8550 IN denotes for
T7284 8551-8552 CD denotes 7
T7285 8553-8554 CD denotes 8
T7286 8555-8556 NN denotes h
T7287 8557-8559 IN denotes in
T7288 8560-8568 JJ denotes complete
T7289 8569-8575 NN denotes medium
T7290 8576-8577 -LRB- denotes (
T7291 8577-8579 CD denotes 10
T7292 8579-8580 NN denotes %
T7293 8581-8587 JJ denotes bovine
T7294 8588-8593 NN denotes serum
T7295 8594-8596 IN denotes in
T7296 8597-8605 NNP denotes RPMI1640
T7297 8605-8608 CD denotes +15
T7298 8609-8620 NN denotes L-glutamine
T7299 8620-8621 -RRB- denotes )
T7300 8622-8631 VBG denotes according
T7301 8632-8634 TO denotes to
T7302 8635-8638 DT denotes the
T7303 8639-8646 JJ denotes general
T7304 8647-8652 NNP denotes Amaxa
T7305 8653-8661 NN denotes protocol
T7306 8662-8665 IN denotes for
T7307 8666-8679 NN denotes nucleofection
T7308 8679-8680 . denotes .
T7309 8681-8684 DT denotes The
T7310 8685-8691 NN denotes medium
T7311 8692-8695 VBD denotes was
T7312 8696-8708 RB denotes subsequently
T7313 8709-8716 VBN denotes changed
T7314 8717-8719 TO denotes to
T7315 8720-8721 CD denotes 1
T7316 8721-8722 NN denotes %
T7317 8723-8727 NNP denotes RPMI
T7318 8728-8731 IN denotes for
T7319 8732-8734 CD denotes 24
T7320 8735-8736 NN denotes h
T7321 8737-8743 IN denotes before
T7322 8744-8755 VBN denotes transfected
T7323 8756-8761 NNS denotes cells
T7324 8762-8766 VBD denotes were
T7325 8767-8772 VBN denotes added
T7326 8773-8775 TO denotes to
T7327 8776-8784 JJ denotes anti-CD3
T7328 8784-8785 NN denotes /
T7329 8785-8789 CD denotes CD28
T7330 8790-8797 VBN denotes treated
T7331 8798-8803 NNS denotes wells
T7332 8804-8807 CC denotes and
T7333 8808-8812 VBP denotes live
T7334 8813-8817 NN denotes cell
T7335 8818-8833 JJ denotes videomicroscopy
T7336 8834-8843 VBN denotes performed
T7337 8843-8844 . denotes .
T7473 8846-8856 NNP denotes Time-Lapse
T7474 8857-8867 NNP denotes Microscopy
T7475 8868-8874 NNP denotes HuT-78
T7476 8875-8880 NNS denotes cells
T7477 8881-8891 VBG denotes expressing
T7478 8892-8902 NNP denotes GATA-3-GFP
T7479 8903-8907 VBD denotes were
T7480 8908-8918 VBN denotes maintained
T7481 8919-8921 IN denotes at
T7482 8922-8924 CD denotes 37
T7483 8924-8925 CD denotes °
T7484 8925-8926 NNP denotes C
T7485 8927-8929 IN denotes in
T7486 8930-8936 NN denotes growth
T7487 8937-8943 NN denotes medium
T7488 8944-8946 IN denotes in
T7489 8947-8948 DT denotes a
T7490 8949-8955 JJ denotes closed
T7491 8956-8960 CD denotes FCS2
T7492 8961-8970 NN denotes perfusion
T7493 8971-8978 NN denotes chamber
T7494 8979-8980 -LRB- denotes (
T7495 8980-8989 NNPS denotes Bioptechs
T7496 8989-8990 , denotes ,
T7497 8991-8997 NNP denotes Butler
T7498 8997-8998 , denotes ,
T7499 8999-9011 NNP denotes Pennsylvania
T7500 9011-9012 , denotes ,
T7501 9013-9016 NNP denotes USA
T7502 9016-9017 -RRB- denotes )
T7503 9018-9026 VBN denotes combined
T7504 9027-9031 IN denotes with
T7505 9032-9034 DT denotes an
T7506 9035-9044 JJ denotes objective
T7507 9045-9051 NN denotes heater
T7508 9052-9053 -LRB- denotes (
T7509 9053-9062 NNPS denotes Bioptechs
T7510 9062-9063 -RRB- denotes )
T7511 9064-9066 IN denotes on
T7512 9067-9070 DT denotes the
T7513 9071-9076 NN denotes stage
T7514 9077-9078 DT denotes a
T7515 9079-9084 NNP denotes Zeiss
T7516 9085-9093 NNP denotes Axiovert
T7517 9094-9097 CD denotes 200
T7518 9098-9108 NN denotes microscope
T7519 9109-9110 -LRB- denotes (
T7520 9110-9119 NNP denotes Thornwood
T7521 9119-9120 , denotes ,
T7522 9121-9124 NNP denotes New
T7523 9125-9129 NNP denotes York
T7524 9129-9130 , denotes ,
T7525 9131-9134 NNP denotes USA
T7526 9134-9135 -RRB- denotes )
T7527 9135-9136 . denotes .
T7528 9137-9149 NNS denotes Observations
T7529 9150-9154 VBD denotes were
T7530 9155-9159 VBN denotes made
T7531 9160-9162 IN denotes by
T7532 9163-9165 CD denotes 40
T7533 9165-9166 CD denotes ×
T7534 9166-9169 CD denotes 1.0
T7535 9170-9172 NNP denotes NA
T7536 9173-9186 NN denotes oil-immersion
T7537 9187-9196 NN denotes objective
T7538 9197-9201 NN denotes lens
T7539 9201-9202 , denotes ,
T7540 9203-9206 CC denotes and
T7541 9207-9219 NN denotes fluorescence
T7542 9220-9223 CC denotes and
T7543 9224-9229 NN denotes phase
T7544 9230-9238 NN denotes contrast
T7545 9239-9245 NNS denotes images
T7546 9246-9250 VBD denotes were
T7547 9251-9259 VBN denotes gathered
T7548 9260-9265 VBG denotes using
T7549 9266-9267 DT denotes a
T7550 9268-9277 NNP denotes Hamamatsu
T7551 9278-9285 NNP denotes ORCA-ER
T7552 9286-9293 VBD denotes charged
T7553 9294-9301 VBN denotes coupled
T7554 9302-9308 NN denotes device
T7555 9309-9315 NN denotes camera
T7556 9316-9317 -LRB- denotes (
T7557 9317-9328 NNP denotes Bridgewater
T7558 9328-9329 , denotes ,
T7559 9330-9333 NNP denotes New
T7560 9334-9340 NNP denotes Jersey
T7561 9340-9341 , denotes ,
T7562 9342-9345 NNP denotes USA
T7563 9345-9346 -RRB- denotes )
T7564 9347-9353 VBN denotes driven
T7565 9354-9356 IN denotes by
T7566 9357-9364 NNP denotes Openlab
T7567 9365-9373 NN denotes software
T7568 9374-9375 -LRB- denotes (
T7569 9375-9386 NNP denotes Improvision
T7570 9386-9387 , denotes ,
T7571 9388-9396 NNP denotes Coventry
T7572 9396-9397 , denotes ,
T7573 9398-9400 NNP denotes UK
T7574 9400-9401 -RRB- denotes )
T7575 9401-9402 . denotes .
T7576 9403-9414 NNS denotes Photographs
T7577 9415-9419 VBD denotes were
T7578 9420-9425 VBN denotes taken
T7579 9426-9428 IN denotes at
T7580 9429-9430 CD denotes 0
T7581 9430-9431 , denotes ,
T7582 9432-9434 CD denotes 30
T7583 9434-9435 , denotes ,
T7584 9436-9438 CD denotes 60
T7585 9438-9439 , denotes ,
T7586 9440-9443 CD denotes 120
T7587 9443-9444 , denotes ,
T7588 9445-9448 CC denotes and
T7589 9449-9452 CD denotes 240
T7590 9453-9456 NN denotes min
T7591 9456-9457 . denotes .
T7691 9459-9472 NNP denotes Densitometric
T7692 9473-9481 NNP denotes Analysis
T7693 9482-9494 NNP denotes Densitometry
T7694 9495-9497 IN denotes of
T7695 9498-9501 NNP denotes ECL
T7696 9502-9513 NNS denotes immunoblots
T7697 9514-9517 VBD denotes was
T7698 9518-9527 VBN denotes performed
T7699 9528-9533 VBG denotes using
T7700 9534-9542 NNP denotes Gelworks
T7701 9543-9545 NNP denotes ID
T7702 9546-9558 JJ denotes intermediate
T7703 9559-9567 NN denotes software
T7704 9568-9569 -LRB- denotes (
T7705 9569-9580 NNP denotes Ultraviolet
T7706 9581-9589 NNPS denotes Products
T7707 9589-9590 , denotes ,
T7708 9591-9605 NNP denotes Cambridgeshire
T7709 9605-9606 , denotes ,
T7710 9607-9609 NNP denotes UK
T7711 9609-9610 -RRB- denotes )
T7712 9610-9611 . denotes .
T7713 9612-9619 RB denotes Briefly
T7714 9619-9620 , denotes ,
T7715 9621-9632 NNS denotes immunoblots
T7716 9633-9637 VBD denotes were
T7717 9638-9645 VBN denotes scanned
T7718 9646-9649 CC denotes and
T7719 9650-9655 NNS denotes gates
T7720 9656-9660 VBD denotes were
T7721 9661-9666 VBN denotes drawn
T7722 9667-9674 RB denotes tightly
T7723 9675-9681 IN denotes around
T7724 9682-9686 DT denotes each
T7725 9687-9691 NN denotes band
T7726 9691-9692 . denotes .
T7727 9693-9703 NN denotes Background
T7728 9704-9710 NNS denotes values
T7729 9711-9715 IN denotes from
T7730 9716-9720 DT denotes each
T7731 9721-9725 NN denotes lane
T7732 9726-9730 VBD denotes were
T7733 9731-9741 VBN denotes subtracted
T7734 9742-9744 TO denotes to
T7735 9745-9754 VB denotes normalize
T7736 9755-9759 DT denotes each
T7737 9760-9771 NN denotes measurement
T7738 9771-9772 . denotes .
T7739 9773-9776 DT denotes The
T7740 9777-9782 NNS denotes bands
T7741 9783-9787 VBD denotes were
T7742 9788-9798 VBN denotes quantified
T7743 9799-9804 VBG denotes using
T7744 9805-9808 DT denotes the
T7745 9809-9817 NNPS denotes Gelworks
T7746 9818-9826 NN denotes software
T7747 9826-9827 . denotes .
T7748 9828-9831 DT denotes All
T7749 9832-9839 JJ denotes Western
T7750 9840-9845 NNS denotes blots
T7751 9846-9850 VBD denotes were
T7752 9851-9858 VBN denotes exposed
T7753 9859-9861 TO denotes to
T7754 9862-9866 NN denotes film
T7755 9867-9870 IN denotes for
T7756 9871-9878 VBG denotes varying
T7757 9879-9886 NNS denotes lengths
T7758 9887-9889 IN denotes of
T7759 9890-9894 NN denotes time
T7760 9894-9895 , denotes ,
T7761 9896-9899 CC denotes and
T7762 9900-9904 RB denotes only
T7763 9905-9910 NNS denotes films
T7764 9911-9921 VBG denotes generating
T7765 9922-9935 JJ denotes subsaturating
T7766 9936-9942 NNS denotes levels
T7767 9943-9945 IN denotes of
T7768 9946-9955 NN denotes intensity
T7769 9956-9960 VBD denotes were
T7770 9961-9969 VBN denotes selected
T7771 9970-9973 IN denotes for
T7772 9974-9987 JJ denotes densitometric
T7773 9988-9998 NN denotes evaluation
T7774 9998-9999 . denotes .
T7934 10001-10012 NNP denotes Statistical
T7935 10013-10021 NNP denotes Analysis
T7936 10022-10026 NNP denotes Data
T7937 10027-10031 IN denotes from
T7938 10032-10037 CD denotes three
T7939 10038-10040 CC denotes or
T7940 10041-10045 JJR denotes more
T7941 10046-10057 JJ denotes independent
T7942 10058-10069 NNS denotes experiments
T7943 10070-10073 VBP denotes are
T7944 10074-10083 VBN denotes presented
T7945 10084-10086 IN denotes as
T7946 10087-10090 DT denotes the
T7947 10091-10095 JJ denotes mean
T7948 10095-10096 NN denotes ±
T7949 10096-10104 NN denotes standard
T7950 10105-10110 NN denotes error
T7951 10111-10113 IN denotes of
T7952 10114-10117 DT denotes the
T7953 10118-10122 NN denotes mean
T7954 10123-10124 -LRB- denotes (
T7955 10124-10127 NNP denotes SEM
T7956 10127-10128 -RRB- denotes )
T7957 10128-10129 , denotes ,
T7958 10130-10136 IN denotes except
T7959 10137-10142 WRB denotes where
T7960 10143-10149 VBN denotes stated
T7961 10150-10153 CC denotes and
T7962 10154-10158 VBD denotes were
T7963 10159-10167 VBN denotes compared
T7964 10168-10173 VBG denotes using
T7965 10174-10182 NNP denotes GraphPad
T7966 10183-10188 NNP denotes Prism
T7967 10189-10190 CD denotes 4
T7968 10191-10192 -LRB- denotes (
T7969 10192-10200 NNP denotes GraphPad
T7970 10201-10209 NNP denotes Software
T7971 10209-10210 , denotes ,
T7972 10211-10234 NN denotes http://www.graphpad.com
T7973 10234-10235 -RRB- denotes )
T7974 10235-10236 . denotes .
T7975 10237-10244 NNS denotes Results
T7976 10245-10249 VBD denotes were
T7977 10250-10258 JJ denotes analysed
T7978 10259-10264 VBG denotes using
T7979 10265-10272 JJ denotes one-way
T7980 10273-10278 NNP denotes ANOVA
T7981 10279-10283 IN denotes with
T7982 10284-10296 NNP denotes Newman-Keuls
T7983 10297-10301 NN denotes post
T7984 10302-10306 NN denotes test
T7985 10307-10313 IN denotes except
T7986 10314-10317 IN denotes for
T7987 10318-10321 DT denotes the
T7988 10322-10326 NNS denotes data
T7989 10327-10331 IN denotes from
T7990 10332-10335 DT denotes the
T7991 10336-10338 IN denotes in
T7992 10339-10343 NN denotes vivo
T7993 10344-10351 VBD denotes inhaled
T7994 10352-10354 NNP denotes FP
T7995 10355-10360 NN denotes study
T7996 10360-10361 , denotes ,
T7997 10362-10367 WDT denotes which
T7998 10368-10371 VBD denotes was
T7999 10372-10380 VBN denotes analysed
T8000 10381-10383 IN denotes by
T8001 10384-10392 NNP denotes Friedman
T8002 10392-10394 POS denotes 's
T8003 10395-10399 NN denotes test
T8004 10400-10404 IN denotes with
T8005 10405-10415 JJ denotes subsequent
T8006 10416-10425 NNP denotes Wilcoxson
T8007 10426-10433 VBD denotes matched
T8008 10434-10438 NN denotes pair
T8009 10439-10445 VBD denotes signed
T8010 10446-10450 NN denotes rank
T8011 10451-10454 NN denotes sum
T8012 10455-10459 NN denotes test
T8013 10459-10460 . denotes .
T8014 10461-10465 NNP denotes Data
T8015 10466-10470 IN denotes from
T8016 10471-10475 DT denotes this
T8017 10476-10484 NN denotes analysis
T8018 10485-10488 VBP denotes are
T8019 10489-10498 VBN denotes presented
T8020 10499-10501 IN denotes as
T8021 10502-10503 DT denotes a
T8022 10504-10520 NNS denotes box-and-whiskers
T8023 10521-10525 NN denotes plot
T8024 10525-10526 . denotes .
T8025 10527-10535 NNP denotes Friedman
T8026 10535-10537 POS denotes 's
T8027 10538-10542 NN denotes test
T8028 10543-10546 VBD denotes was
T8029 10547-10551 VBN denotes used
T8030 10552-10554 IN denotes as
T8031 10555-10560 CD denotes three
T8032 10561-10568 VBN denotes matched
T8033 10569-10577 NNS denotes measures
T8034 10578-10582 VBD denotes were
T8035 10583-10591 VBN denotes obtained
T8036 10592-10597 VBG denotes using
T8037 10598-10605 NN denotes placebo
T8038 10605-10606 , denotes ,
T8039 10607-10610 CD denotes 100
T8040 10611-10613 NN denotes µg
T8041 10613-10614 , denotes ,
T8042 10615-10618 CC denotes and
T8043 10619-10622 CD denotes 500
T8044 10623-10625 NN denotes µg
T8045 10626-10628 IN denotes of
T8046 10629-10636 JJ denotes inhaled
T8047 10637-10639 NNP denotes FP
T8048 10639-10640 , denotes ,
T8049 10641-10646 WDT denotes which
T8050 10647-10650 VBD denotes had
T8051 10651-10659 JJ denotes variable
T8052 10660-10668 NN denotes baseline
T8053 10669-10675 NNS denotes levels
T8054 10675-10676 . denotes .
T8055 10677-10679 PRP denotes We
T8056 10680-10683 VBD denotes did
T8057 10684-10687 RB denotes not
T8058 10688-10694 VB denotes assume
T8059 10695-10696 DT denotes a
T8060 10697-10705 JJ denotes Gaussian
T8061 10706-10718 NN denotes distribution
T8062 10719-10721 IN denotes of
T8063 10722-10725 DT denotes the
T8064 10726-10730 NNS denotes data
T8065 10731-10734 JJ denotes due
T8066 10735-10737 TO denotes to
T8067 10738-10741 DT denotes the
T8068 10742-10749 JJ denotes limited
T8069 10750-10757 NNS denotes numbers
T8070 10758-10760 IN denotes of
T8071 10761-10773 NNS denotes participants
T8072 10774-10782 VBN denotes analysed
T8073 10783-10784 -LRB- denotes (
T8074 10784-10789 CD denotes seven
T8075 10789-10790 -RRB- denotes )
T8076 10790-10791 . denotes .
T8077 10791-10794 DT denotes The
T8078 10795-10799 NN denotes null
T8079 10800-10810 NN denotes hypothesis
T8080 10811-10814 VBD denotes was
T8081 10815-10823 VBN denotes rejected
T8082 10824-10826 IN denotes at
T8083 10827-10828 NN denotes p
T8084 10828-10829 NN denotes <
T8085 10829-10833 CD denotes 0.05
T8086 10833-10834 . denotes .
R3612 T4177 T4174 punct ),GATA-3
R3613 T4178 T4174 cc and,GATA-3
R3614 T4179 T4174 conj GR,GATA-3
R3615 T4180 T4181 punct (,E-20
R3616 T4181 T4174 conj E-20,GATA-3
R3617 T4182 T4181 punct ",",E-20
R3618 T4183 T4181 amod sc-1003,E-20
R3619 T4184 T4181 punct ),E-20
R3620 T4185 T4186 auxpass were,obtained
R3621 T4186 T4186 ROOT obtained,obtained
R3622 T4187 T4186 prep from,obtained
R3623 T4188 T4190 compound Santa,Biotechnology
R3624 T4189 T4190 compound Cruz,Biotechnology
R3625 T4190 T4187 pobj Biotechnology,from
R3626 T4191 T4190 punct (,Biotechnology
R3627 T4192 T4193 compound Santa,Cruz
R3628 T4193 T4190 appos Cruz,Biotechnology
R3629 T4194 T4193 punct ",",Cruz
R3630 T4195 T4193 conj California,Cruz
R3631 T4196 T4195 punct ",",California
R3632 T4197 T4198 compound United,States
R3633 T4198 T4195 appos States,California
R3634 T4199 T4195 punct ),California
R3635 T4200 T4190 punct ",",Biotechnology
R3636 T4201 T4203 amod polyclonal,antibodies
R3637 T4202 T4203 compound rabbit,antibodies
R3638 T4203 T4186 dobj antibodies,obtained
R3639 T4204 T4186 prep against,obtained
R3640 T4205 T4207 amod phospho-p38,kinase
R3641 T4206 T4207 compound MAP,kinase
R3642 T4207 T4204 pobj kinase,against
R3643 T4208 T4207 cc and,kinase
R3644 T4209 T4207 conj phospho-ATF-2,kinase
R3645 T4210 T4207 prep from,kinase
R3646 T4211 T4212 compound Cell,Signaling
R3647 T4212 T4210 pobj Signaling,from
R3648 T4213 T4225 nmod Technology,antibody
R3649 T4214 T4217 punct (,Biolabs
R3650 T4215 T4217 compound New,Biolabs
R3651 T4216 T4217 compound England,Biolabs
R3652 T4217 T4213 appos Biolabs,Technology
R3653 T4218 T4217 punct ",",Biolabs
R3654 T4219 T4217 conj Hertford,Biolabs
R3655 T4220 T4219 punct ",",Hertford
R3656 T4221 T4219 conj UK,Hertford
R3657 T4222 T4217 punct ),Biolabs
R3658 T4223 T4217 punct ",",Biolabs
R3659 T4224 T4225 amod monoclonal,antibody
R3660 T4225 T4243 nsubj antibody,antibodies
R3661 T4226 T4225 prep against,antibody
R3662 T4227 T4226 pobj phosphoserine,against
R3663 T4228 T4230 punct (,4H4
R3664 T4229 T4230 compound clone,4H4
R3665 T4230 T4227 appos 4H4,phosphoserine
R3666 T4231 T4230 punct ),4H4
R3667 T4232 T4230 prep from,4H4
R3668 T4233 T4235 compound Affiniti,Products
R3669 T4234 T4235 compound Research,Products
R3670 T4235 T4232 pobj Products,from
R3671 T4236 T4235 punct (,Products
R3672 T4237 T4235 npadvmod Exeter,Products
R3673 T4238 T4237 punct ",",Exeter
R3674 T4239 T4237 appos UK,Exeter
R3675 T4240 T4237 punct ),Exeter
R3676 T4241 T4235 punct ",",Products
R3677 T4242 T4227 cc and,phosphoserine
R3678 T4243 T4212 dobj antibodies,Signaling
R3679 T4244 T4243 prep against,antibodies
R3680 T4245 T4247 compound rabbit,TRITC
R3681 T4246 T4247 compound IgG-conjugated,TRITC
R3682 T4247 T4244 pobj TRITC,against
R3683 T4248 T4247 prep from,TRITC
R3684 T4249 T4250 compound Dako,Cytomation
R3685 T4250 T4248 pobj Cytomation,from
R3686 T4251 T4250 punct (,Cytomation
R3687 T4252 T4250 appos Cambridge,Cytomation
R3688 T4253 T4252 punct ",",Cambridge
R3689 T4254 T4252 appos UK,Cambridge
R3690 T4255 T4252 punct ),Cambridge
R3691 T4256 T4186 punct .,obtained
R3692 T4257 T4259 det The,antibody
R3693 T4258 T4259 amod anti-GR,antibody
R3694 T4259 T4274 nsubjpass antibody,used
R3695 T4260 T4259 punct (,antibody
R3696 T4261 T4259 appos Clone,antibody
R3697 T4262 T4261 nummod 41,Clone
R3698 T4263 T4261 punct ),Clone
R3699 T4264 T4259 prep from,antibody
R3700 T4265 T4267 compound BD,Laboratories
R3701 T4266 T4267 compound Transduction,Laboratories
R3292 T3810 T3815 npadvmod Participants,studied
R3293 T3811 T3810 cc and,Participants
R3294 T3812 T3813 compound Study,Design
R3295 T3813 T3810 conj Design,Participants
R3296 T3814 T3815 nsubj We,studied
R3297 T3815 T3815 ROOT studied,studied
R3298 T3816 T3815 dobj patients,studied
R3299 T3817 T3816 prep with,patients
R3300 T3818 T3819 amod mild,asthma
R3301 T3819 T3817 pobj asthma,with
R3302 T3820 T3823 nsubjpass who,treated
R3303 T3821 T3823 auxpass were,treated
R3304 T3822 T3823 neg not,treated
R3305 T3823 T3816 relcl treated,patients
R3306 T3824 T3823 prep with,treated
R3307 T3825 T3826 amod inhaled,corticosteroids
R3308 T3826 T3824 pobj corticosteroids,with
R3309 T3827 T3830 nsubjpass who,included
R3310 T3828 T3830 aux had,included
R3311 T3829 T3830 auxpass been,included
R3312 T3830 T3826 relcl included,corticosteroids
R3313 T3831 T3830 prep in,included
R3314 T3832 T3840 det a,study
R3315 T3833 T3834 advmod previously,reported
R3316 T3834 T3835 amod reported,double-blind
R3317 T3835 T3840 amod double-blind,study
R3318 T3836 T3840 punct ",",study
R3319 T3837 T3840 amod placebo-controlled,study
R3320 T3838 T3840 punct ",",study
R3321 T3839 T3840 amod crossover,study
R3322 T3840 T3831 pobj study,in
R3323 T3841 T3840 prep with,study
R3324 T3842 T3843 compound FP,[
R3325 T3843 T3841 pobj [,with
R3326 T3844 T3845 nummod 34,]
R3327 T3845 T3843 appos ],[
R3328 T3846 T3815 punct .,studied
R3329 T3847 T3848 nummod Seven,patients
R3330 T3848 T3852 nsubj patients,entered
R3331 T3849 T3848 prep with,patients
R3332 T3850 T3851 amod mild,asthma
R3333 T3851 T3849 pobj asthma,with
R3334 T3852 T3852 ROOT entered,entered
R3335 T3853 T3854 det the,study
R3336 T3854 T3852 dobj study,entered
R3337 T3855 T3852 cc and,entered
R3338 T3856 T3857 auxpass were,randomized
R3339 T3857 T3852 conj randomized,entered
R3340 T3858 T3859 aux to,receive
R3341 T3859 T3857 xcomp receive,randomized
R3342 T3860 T3862 det a,inhalation
R3343 T3861 T3862 amod single,inhalation
R3344 T3862 T3859 dobj inhalation,receive
R3345 T3863 T3862 prep of,inhalation
R3346 T3864 T3863 pobj FP,of
R3347 T3865 T3864 punct (,FP
R3348 T3866 T3864 nummod 100,FP
R3349 T3867 T3864 cc and,FP
R3350 T3868 T3869 nummod 500,µg
R3351 T3869 T3864 conj µg,FP
R3352 T3870 T3869 punct ),µg
R3353 T3871 T3869 cc or,µg
R3354 T3872 T3875 det a,control
R3355 T3873 T3875 amod matched,control
R3356 T3874 T3875 compound placebo,control
R3357 T3875 T3869 conj control,µg
R3358 T3876 T3859 prep via,receive
R3359 T3877 T3879 det a,chamber
R3360 T3878 T3879 compound spacer,chamber
R3361 T3879 T3876 pobj chamber,via
R3362 T3880 T3857 punct ",",randomized
R3363 T3881 T3857 cc and,randomized
R3364 T3882 T3884 det the,treatment
R3365 T3883 T3884 amod other,treatment
R3366 T3884 T3886 nsubjpass treatment,given
R3367 T3885 T3886 auxpass was,given
R3368 T3886 T3857 conj given,randomized
R3369 T3887 T3898 mark after,taken
R3370 T3888 T3890 det a,period
R3371 T3889 T3890 amod wash-out,period
R3372 T3890 T3898 nsubjpass period,taken
R3373 T3891 T3890 prep of,period
R3374 T3892 T3893 advmod at,least
R3375 T3893 T3894 advmod least,6
R3376 T3894 T3895 nummod 6,d.
R3377 T3895 T3896 compound d.,Blood
R3378 T3896 T3891 pobj Blood,of
R3389 T3907 T3898 conj h,taken
R3390 T3908 T3907 prep after,h
R3391 T3909 T3910 compound drug,administration
R3392 T3910 T3908 pobj administration,after
R3393 T3911 T3852 punct .,entered
R3394 T3912 T3913 det All,patients
R3395 T3913 T3914 nsubj patients,gave
R3396 T3914 T3914 ROOT gave,gave
R3397 T3915 T3916 amod informed,consent
R3398 T3916 T3914 dobj consent,gave
R3399 T3917 T3916 cc and,consent
R3400 T3918 T3919 det the,study
R3401 T3919 T3921 nsubjpass study,approved
R3402 T3920 T3921 auxpass was,approved
R3403 T3921 T3914 conj approved,gave
R3404 T3922 T3921 agent by,approved
R3405 T3923 T3925 det the,Committee
R3406 T3924 T3925 compound Ethics,Committee
R3407 T3925 T3922 pobj Committee,by
R3408 T3926 T3925 prep of,Committee
R3409 T3927 T3929 det the,Brompton
R3410 T3928 T3929 compound Royal,Brompton
R3411 T3929 T3926 pobj Brompton,of
R3412 T3930 T3929 cc and,Brompton
R3413 T3931 T3934 compound Harefield,Trust
R3414 T3932 T3934 compound Hospitals,Trust
R3415 T3933 T3934 compound NHS,Trust
R3416 T3934 T3929 conj Trust,Brompton
R3417 T3935 T3921 punct .,approved
R3418 T3936 T3938 det The,study
R3419 T3937 T3938 amod clinical,study
R3420 T3938 T3940 nsubjpass study,conducted
R3421 T3939 T3940 auxpass was,conducted
R3422 T3940 T3940 ROOT conducted,conducted
R3423 T3941 T3940 prep before,conducted
R3424 T3942 T3943 det the,requirement
R3425 T3943 T3941 pobj requirement,before
R3426 T3944 T3943 prep for,requirement
R3427 T3945 T3947 compound Clinical,Registration
R3428 T3946 T3947 compound Trial,Registration
R3429 T3947 T3944 pobj Registration,for
R3430 T3948 T3940 punct .,conducted
R3577 T4142 T4142 ROOT Antibodies,Antibodies
R3578 T4143 T4142 cc and,Antibodies
R3579 T4144 T4142 conj Reagents,Antibodies
R3580 T4145 T4147 det The,antibodies
R3581 T4146 T4147 amod monoclonal,antibodies
R3582 T4147 T4159 nsubjpass antibodies,purchased
R3583 T4148 T4147 prep against,antibodies
R3584 T4149 T4150 compound human,CD3
R3585 T4150 T4148 pobj CD3,against
R3586 T4151 T4150 punct ",",CD3
R3587 T4152 T4150 conj CD28,CD3
R3588 T4153 T4152 punct ",",CD28
R3589 T4154 T4152 conj GR,CD28
R3590 T4155 T4154 punct ",",GR
R3591 T4156 T4154 cc and,GR
R3592 T4157 T4154 conj importin-α,GR
R3593 T4158 T4159 auxpass were,purchased
R3594 T4159 T4159 ROOT purchased,purchased
R3595 T4160 T4159 prep from,purchased
R3596 T4161 T4162 compound BD,Biosciences
R3597 T4162 T4160 pobj Biosciences,from
R3598 T4163 T4162 punct (,Biosciences
R3599 T4164 T4162 npadvmod Oxford,Biosciences
R3600 T4165 T4164 punct ",",Oxford
R3601 T4166 T4167 compound United,Kingdom
R3602 T4167 T4164 appos Kingdom,Oxford
R3604 T4169 T4159 punct .,purchased
R3605 T4170 T4171 compound Rabbit,antibodies
R3606 T4171 T4186 nsubjpass antibodies,obtained
R3607 T4172 T4171 prep against,antibodies
R3608 T4173 T4174 compound human,GATA-3
R3609 T4174 T4172 pobj GATA-3,against
R3610 T4175 T4174 punct (,GATA-3
R3611 T4176 T4174 appos H-48,GATA-3
R3702 T4267 T4264 pobj Laboratories,from
R3703 T4268 T4267 punct (,Laboratories
R3704 T4269 T4267 npadvmod Oxford,Laboratories
R3705 T4270 T4269 punct ",",Oxford
R3706 T4271 T4269 appos UK,Oxford
R3707 T4272 T4269 punct ),Oxford
R3708 T4273 T4274 auxpass was,used
R3709 T4274 T4274 ROOT used,used
R3710 T4275 T4274 prep for,used
R3711 T4276 T4278 amod Western,analysis
R3712 T4277 T4278 compound blot,analysis
R3713 T4278 T4275 pobj analysis,for
R3714 T4279 T4274 punct .,used
R3715 T4280 T4282 det All,reagents
R3716 T4281 T4282 amod other,reagents
R3727 T4292 T4284 punct .,purchased
R3841 T4470 T4471 compound Cell,Culture
R3842 T4471 T4474 nsubj Culture,Isolation
R3843 T4472 T4471 cc and,Culture
R3844 T4473 T4471 conj PBMC,Culture
R3845 T4474 T4498 nsubjpass Isolation,cultured
R3846 T4475 T4479 det A,line
R3847 T4476 T4478 amod human,cell
R3848 T4477 T4478 compound T,cell
R3849 T4478 T4479 compound cell,line
R3850 T4479 T4474 dobj line,Isolation
R3851 T4480 T4484 punct (,purchased
R3852 T4481 T4484 nsubjpass HuT-78,purchased
R3853 T4482 T4481 punct ),HuT-78
R3854 T4483 T4484 auxpass was,purchased
R3855 T4484 T4474 relcl purchased,Isolation
R3856 T4485 T4484 prep from,purchased
R3857 T4486 T4488 compound ECACC,Collection
R3858 T4487 T4488 compound European,Collection
R3859 T4488 T4485 pobj Collection,from
R3860 T4489 T4488 prep of,Collection
R3861 T4490 T4491 compound Cell,Culture
R3862 T4491 T4489 pobj Culture,of
R3863 T4492 T4488 punct (,Collection
R3864 T4493 T4488 appos Wiltshire,Collection
R3865 T4494 T4493 punct ",",Wiltshire
R3866 T4495 T4493 appos UK,Wiltshire
R3867 T4496 T4493 punct ),Wiltshire
R3868 T4497 T4498 auxpass were,cultured
R3869 T4498 T4498 ROOT cultured,cultured
R3870 T4499 T4501 mark as,described
R3871 T4500 T4501 advmod previously,described
R3872 T4501 T4498 advcl described,cultured
R3873 T4502 T4504 nmod [,]
R3874 T4503 T4504 nummod 12,]
R3875 T4504 T4501 dobj ],described
R3876 T4505 T4498 punct .,cultured
R3877 T4506 T4508 nsubjpass PBMCs,isolated
R3878 T4507 T4508 auxpass were,isolated
R3879 T4508 T4529 ccomp isolated,described
R3880 T4509 T4508 agent by,isolated
R3881 T4510 T4511 compound density,centrifugation
R3882 T4511 T4509 pobj centrifugation,by
R3883 T4512 T4511 prep over,centrifugation
R3884 T4513 T4512 pobj Ficoll-Hypaque,over
R3885 T4514 T4515 punct (,density
R3886 T4515 T4513 appos density,Ficoll-Hypaque
R3887 T4516 T4515 punct ",",density
R3888 T4517 T4518 nummod 1.077,g/ml
R3889 T4518 T4515 appos g/ml,density
R3890 T4519 T4529 punct ;,described
R3891 T4520 T4521 compound Amersham,Biosciences
R3892 T4521 T4529 nsubj Biosciences,described
R3893 T4522 T4521 punct ",",Biosciences
R3894 T4523 T4521 conj Amersham,Biosciences
R3895 T4524 T4523 punct ",",Amersham
R3896 T4525 T4523 conj UK,Amersham
R3897 T4526 T4521 punct ),Biosciences
R3898 T4527 T4529 mark as,described
R3899 T4528 T4529 advmod previously,described
R3900 T4529 T4529 ROOT described,described
R3901 T4530 T4532 nmod [,]
R3902 T4531 T4532 nummod 34,]
R3903 T4532 T4529 dobj ],described
R3904 T4533 T4529 punct .,described
R3905 T4534 T4536 nsubjpass Cells,stimulated
R3906 T4535 T4536 auxpass were,stimulated
R3907 T4536 T4536 ROOT stimulated,stimulated
R3908 T4537 T4536 prep with,stimulated
R3909 T4538 T4540 amod anti-CD3,CD28
R3910 T4539 T4540 compound /,CD28
R3911 T4540 T4537 pobj CD28,with
R3912 T4541 T4540 punct (,CD28
R3913 T4542 T4543 nummod 1,µg
R3914 T4543 T4545 nmod µg,ml
R3915 T4544 T4545 compound /,ml
R3916 T4545 T4540 appos ml,CD28
R3917 T4546 T4545 npadvmod each,ml
R3918 T4547 T4540 punct ),CD28
R3919 T4548 T4536 prep for,stimulated
R3920 T4549 T4550 nummod 1,h
R3921 T4550 T4548 pobj h,for
R3922 T4551 T4550 prep at,h
R3923 T4552 T4553 nummod 37,°
R3924 T4553 T4551 pobj °,at
R3925 T4554 T4550 npadvmod C,h
R3926 T4555 T4556 aux to,stimulate
R3927 T4556 T4536 advcl stimulate,stimulated
R3928 T4557 T4559 amod Th2,release
R3929 T4558 T4559 compound cytokine,release
R3930 T4559 T4556 dobj release,stimulate
R3931 T4560 T4559 prep in,release
R3932 T4561 T4562 det the,presence
R3933 T4562 T4560 pobj presence,in
R3934 T4563 T4562 cc or,presence
R3935 T4564 T4562 conj absence,presence
R3936 T4565 T4562 prep of,presence
R3937 T4566 T4565 pobj FP,of
R3938 T4567 T4569 punct (,−
R3939 T4568 T4569 nummod 10,−
R3940 T4569 T4566 appos −,FP
R3941 T4570 T4572 quantmod 12,10
R3942 T4571 T4572 quantmod to,10
R3943 T4572 T4574 nummod 10,8M
R3944 T4573 T4574 nummod −,8M
R3945 T4574 T4569 npadvmod 8M,−
R3946 T4575 T4569 punct ),−
R3947 T4576 T4536 punct .,stimulated
R3948 T4577 T4579 nsubjpass Cytospins,prepared
R3949 T4578 T4579 auxpass were,prepared
R3950 T4579 T4582 amod prepared,localization
R3951 T4580 T4579 cc and,prepared
R3952 T4581 T4579 conj GATA-3,prepared
R3953 T4582 T4582 ROOT localization,localization
R3954 T4583 T4582 acl determined,localization
R3955 T4584 T4583 agent by,determined
R3956 T4585 T4586 amod confocal,microscopy
R3957 T4586 T4584 pobj microscopy,by
R3958 T4587 T4589 mark as,described
R3959 T4588 T4589 advmod previously,described
R3960 T4589 T4582 advcl described,localization
R3961 T4590 T4592 nmod [,]
R3962 T4591 T4592 nummod 12,]
R3963 T4592 T4589 dobj ],described
R3964 T4593 T4582 punct .,localization
R4061 T4717 T4721 amod Reverse,RNA
R4062 T4718 T4721 compound Transcription,RNA
R4063 T4719 T4721 compound PCR,RNA
R4064 T4720 T4721 compound Total,RNA
R4065 T4721 T4723 nsubjpass RNA,extracted
R4066 T4722 T4723 auxpass was,extracted
R4067 T4723 T4723 ROOT extracted,extracted
R4068 T4724 T4723 advcl using,extracted
R4069 T4725 T4726 compound lysis,buffer
R4070 T4726 T4724 dobj buffer,using
R4071 T4727 T4726 punct (,buffer
R4072 T4728 T4729 compound RNeasy,kit
R4073 T4729 T4726 appos kit,buffer
R4074 T4730 T4726 punct ;,buffer
R4075 T4731 T4726 conj Qiagen,buffer
R4076 T4732 T4731 punct ",",Qiagen
R4077 T4733 T4731 conj Crawley,Qiagen
R4078 T4734 T4733 punct ",",Crawley
R4079 T4735 T4733 conj UK,Crawley
R4080 T4736 T4731 punct ),Qiagen
R4081 T4737 T4723 punct .,extracted
R4082 T4738 T4745 prep During,digested
R4083 T4739 T4740 compound RNA,purification
R4084 T4740 T4738 pobj purification,During
R4085 T4741 T4745 punct ",",digested
R4086 T4742 T4743 amod genomic,DNA
R4087 T4743 T4745 nsubjpass DNA,digested
R4088 T4744 T4745 auxpass was,digested
R4089 T4745 T4745 ROOT digested,digested
R4090 T4746 T4745 prep with,digested
R4091 T4747 T4748 compound RNase-free,DNase
R4092 T4748 T4746 pobj DNase,with
R4093 T4749 T4751 punct (,Biosciences
R4094 T4750 T4751 compound Amersham,Biosciences
R4095 T4751 T4748 appos Biosciences,DNase
R4096 T4752 T4751 punct ),Biosciences
R4097 T4753 T4745 punct .,digested
R4098 T4754 T4757 advmod Next,µg
R4099 T4755 T4757 punct ",",µg
R4100 T4756 T4757 nummod 0.5,µg
R4101 T4757 T4763 nsubjpass µg,transcribed
R4102 T4758 T4757 prep of,µg
R4103 T4759 T4760 amod total,RNA
R4104 T4760 T4758 pobj RNA,of
R4105 T4761 T4763 auxpass was,transcribed
R4106 T4762 T4763 advmod reversed,transcribed
R4107 T4763 T4763 ROOT transcribed,transcribed
R4108 T4764 T4763 advcl using,transcribed
R4109 T4765 T4768 det the,virus
R4110 T4766 T4767 amod avian,myeloblastosis
R4111 T4767 T4768 compound myeloblastosis,virus
R4134 T4790 T4793 nsubj Primers,were
R4135 T4791 T4790 prep for,Primers
R4136 T4792 T4791 pobj IL-4,for
R4137 T4793 T4793 ROOT were,were
R4138 T4794 T4793 prep from,were
R4139 T4795 T4794 pobj Sigma-Genosys,from
R4140 T4796 T4795 punct (,Sigma-Genosys
R4141 T4797 T4795 appos Cambridge,Sigma-Genosys
R4142 T4798 T4797 punct ",",Cambridge
R4143 T4799 T4797 appos UK,Cambridge
R4144 T4800 T4797 punct ),Cambridge
R4145 T4801 T4793 punct .,were
R4146 T4802 T4806 nsubj Sequences,are
R4147 T4803 T4802 prep of,Sequences
R4148 T4804 T4803 pobj GADPH,of
R4149 T4805 T4802 acl used,Sequences
R4150 T4806 T4806 ROOT are,are
R4151 T4807 T4808 mark as,follows
R4152 T4808 T4806 advcl follows,are
R4153 T4809 T4806 punct :,are
R4154 T4810 T4816 advmod forward,reverse
R4155 T4811 T4814 nummod 5,CCACCCATGGCAAATTCCATGGC
R4156 T4812 T4814 punct ′,CCACCCATGGCAAATTCCATGGC
R4157 T4813 T4814 punct -,CCACCCATGGCAAATTCCATGGC
R4158 T4814 T4816 npadvmod CCACCCATGGCAAATTCCATGGC,reverse
R4159 T4815 T4816 punct ",",reverse
R4160 T4816 T4820 dep reverse,TCTAGACGGCAGGTCAGGTCCAC
R4161 T4817 T4816 dobj 3,reverse
R4162 T4818 T4820 punct ′,TCTAGACGGCAGGTCAGGTCCAC
R4163 T4819 T4820 punct -,TCTAGACGGCAGGTCAGGTCCAC
R4164 T4820 T4820 ROOT TCTAGACGGCAGGTCAGGTCCAC,TCTAGACGGCAGGTCAGGTCCAC
R4165 T4821 T4820 punct .,TCTAGACGGCAGGTCAGGTCCAC
R4322 T5013 T5014 compound Cell,Fractionation
R4323 T5014 T5027 nsubjpass Fractionation,prepared
R4324 T5015 T5014 punct ",",Fractionation
R4325 T5016 T5014 conj Immunoprecipitation,Fractionation
R4326 T5017 T5016 punct ",",Immunoprecipitation
R4327 T5018 T5016 cc and,Immunoprecipitation
R4328 T5019 T5022 amod Western,Nuclear
R4329 T5020 T5022 compound Blot,Nuclear
R4330 T5021 T5022 compound Analysis,Nuclear
R4331 T5022 T5016 conj Nuclear,Immunoprecipitation
R4332 T5023 T5022 cc and,Nuclear
R4333 T5024 T5022 conj cytoplasmic,Nuclear
R4334 T5025 T5014 appos fractions,Fractionation
R4335 T5026 T5027 auxpass were,prepared
R4336 T5027 T5027 ROOT prepared,prepared
R4337 T5028 T5030 mark as,described
R4338 T5029 T5030 advmod previously,described
R4339 T5030 T5027 advcl described,prepared
R4340 T5031 T5033 nmod [,]
R4341 T5032 T5033 nummod 35,]
R4342 T5033 T5030 dobj ],described
R4343 T5034 T5027 punct .,prepared
R4344 T5035 T5037 amod Whole,lysates
R4345 T5036 T5037 compound cell,lysates
R4346 T5037 T5039 nsubjpass lysates,prepared
R4347 T5038 T5039 auxpass were,prepared
R4348 T5039 T5039 ROOT prepared,prepared
R4349 T5040 T5039 prep in,prepared
R4350 T5041 T5043 compound NP-40,buffer
R4351 T5042 T5043 compound lysis,buffer
R4352 T5043 T5040 pobj buffer,in
R4353 T5044 T5048 punct (,P-40
R4354 T5045 T5046 nummod 0.5,%
R4355 T5046 T5048 compound %,P-40
R4356 T5047 T5048 compound Nonidet,P-40
R4357 T5048 T5043 appos P-40,buffer
R4358 T5049 T5048 punct ",",P-40
R4359 T5050 T5054 nummod 20,pH
R4360 T5051 T5054 compound mM,pH
R4361 T5052 T5054 compound Tris-HCl,pH
R4415 T5106 T5104 conj importin-α,GATA-3
R4438 T5129 T5128 punct ",",Placid
R4439 T5130 T5131 compound New,York
R4440 T5131 T5128 conj York,Placid
R4441 T5132 T5131 punct ",",York
R4442 T5133 T5131 conj USA,York
R4443 T5134 T5125 punct ),Biotechnology
R4444 T5135 T5096 punct .,immunoprecipitated
R4445 T5136 T5138 amod Western,analysis
R4446 T5137 T5138 compound blot,analysis
R4447 T5138 T5140 nsubjpass analysis,performed
R4448 T5139 T5140 auxpass was,performed
R4449 T5140 T5140 ROOT performed,performed
R4450 T5141 T5140 advcl using,performed
R4451 T5142 T5141 dobj anti-GATA-3,using
R4452 T5143 T5142 punct ",",anti-GATA-3
R4453 T5144 T5142 conj anti-importin-α,anti-GATA-3
R4454 T5145 T5144 punct ",",anti-importin-α
R4455 T5146 T5144 conj anti-GR,anti-importin-α
R4456 T5147 T5146 punct ",",anti-GR
R4457 T5148 T5150 amod anti-p-p38,kinase
R4458 T5149 T5150 compound MAP,kinase
R4459 T5150 T5146 conj kinase,anti-GR
R4460 T5151 T5150 punct ",",kinase
R4461 T5152 T5150 conj anti-p-ATF-2,kinase
R4462 T5153 T5152 punct ",",anti-p-ATF-2
R4463 T5154 T5152 cc and,anti-p-ATF-2
R4464 T5155 T5152 conj anti-p-serine,anti-p-ATF-2
R4465 T5156 T5140 punct .,performed
R4466 T5157 T5158 amod Immunoreactive,proteins
R4467 T5158 T5160 nsubjpass proteins,detected
R4468 T5159 T5160 auxpass were,detected
R4469 T5160 T5160 ROOT detected,detected
R4470 T5161 T5160 advcl using,detected
R4471 T5162 T5166 det an,kit
R4472 T5163 T5166 amod enhanced,kit
R4473 T5164 T5165 compound chemiluminescence,ECL
R4474 T5165 T5166 compound ECL,kit
R4475 T5166 T5161 dobj kit,using
R4476 T5167 T5166 punct (,kit
R4477 T5168 T5169 compound Amersham,Biosciences
R4478 T5169 T5166 appos Biosciences,kit
R4479 T5170 T5166 punct ),kit
R4480 T5171 T5160 punct .,detected
R4593 T5318 T5321 compound Chromatin,immunoprecipitation
R4594 T5319 T5321 compound Immunoprecipitation,immunoprecipitation
R4595 T5320 T5321 compound Chromatin,immunoprecipitation
R4596 T5321 T5326 nsubjpass immunoprecipitation,performed
R4597 T5322 T5323 punct (,IP
R4598 T5323 T5321 appos IP,immunoprecipitation
R4599 T5324 T5321 punct ),immunoprecipitation
R4600 T5325 T5326 auxpass was,performed
R4601 T5326 T5326 ROOT performed,performed
R4602 T5327 T5329 mark as,described
R4603 T5328 T5329 advmod previously,described
R4604 T5329 T5326 advcl described,performed
R4605 T5330 T5332 nmod [,]
R4606 T5331 T5332 nummod 12,]
R4607 T5332 T5329 dobj ],described
R4608 T5333 T5329 prep in,described
R4609 T5334 T5335 compound HuT-78,T-cells
R4610 T5335 T5333 pobj T-cells,in
R4611 T5336 T5335 prep with,T-cells
R4612 T5337 T5338 nummod 2,µg
R4613 T5338 T5336 pobj µg,with
R4614 T5339 T5338 prep of,µg
R4615 T5340 T5339 pobj anti-GATA-3,of
R4616 T5341 T5344 punct (,Biotechnology
R4617 T5342 T5344 compound Santa,Biotechnology
R4618 T5343 T5344 compound Cruz,Biotechnology
R4619 T5344 T5340 appos Biotechnology,anti-GATA-3
R4620 T5345 T5344 punct ),Biotechnology
R4621 T5346 T5344 punct ",",Biotechnology
R4622 T5347 T5344 cc or,Biotechnology
R4623 T5348 T5350 amod isotypic,G
R4624 T5349 T5350 compound immunoglobulin,G
R4625 T5350 T5344 conj G,Biotechnology
R4626 T5351 T5338 prep as,µg
R4627 T5352 T5354 det a,control
R4628 T5353 T5354 amod non-specific,control
R4629 T5354 T5351 pobj control,as
R4630 T5355 T5358 punct (,Biotechnology
R4631 T5356 T5357 compound Santa,Cruz
R4632 T5357 T5358 compound Cruz,Biotechnology
R4633 T5358 T5354 appos Biotechnology,control
R4634 T5359 T5354 punct ),control
R4635 T5360 T5329 advmod overnight,described
R4636 T5361 T5329 prep at,described
R4637 T5362 T5363 nummod 4,°
R4638 T5363 T5366 nummod °,sequences
R4639 T5364 T5365 compound C.,Promoter
R4640 T5365 T5366 compound Promoter,sequences
R4641 T5366 T5368 nsubjpass sequences,detected
R4642 T5367 T5368 auxpass were,detected
R4643 T5368 T5326 conj detected,performed
R4644 T5369 T5368 prep with,detected
R4645 T5370 T5371 compound PCR,primers
R4646 T5371 T5369 pobj primers,with
R4647 T5372 T5371 prep for,primers
R4648 T5373 T5375 det the,promoter
R4649 T5374 T5375 compound IL-5,promoter
R4650 T5375 T5372 pobj promoter,for
R4651 T5376 T5375 punct (,promoter
R4652 T5377 T5388 advmod −,′
R4653 T5378 T5388 meta 445,′
R4654 T5379 T5378 prep to,445
R4655 T5380 T5379 pobj +4,to
R4656 T5381 T5378 punct ),445
R4657 T5382 T5388 punct :,′
R4658 T5383 T5388 advmod forward,′
R4659 T5384 T5388 nummod 5,′
R4660 T5385 T5387 punct ′,TTAATCTAGCCACAGTCATAG-3
R4661 T5386 T5387 punct -,TTAATCTAGCCACAGTCATAG-3
R4662 T5387 T5388 compound TTAATCTAGCCACAGTCATAG-3,′
R4663 T5388 T5375 appos ′,promoter
R4664 T5389 T5388 cc and,′
R4665 T5390 T5388 conj reverse,′
R4666 T5391 T5388 punct :,′
R4667 T5392 T5395 nummod 5,TCATGGCTCTGAAACGTTCTG-3
R4687 T5412 T5410 appos UK,Ashford
R4688 T5413 T5408 punct ),Hybaid
R4689 T5414 T5408 prep with,Hybaid
R4690 T5415 T5416 compound cycling,parameters
R4691 T5416 T5414 pobj parameters,with
R4692 T5417 T5416 prep of,parameters
R4693 T5418 T5420 nummod 72,C
R4694 T5419 T5420 compound °,C
R4695 T5420 T5417 pobj C,of
R4696 T5421 T5401 prep for,using
R4697 T5422 T5423 nummod 10,min
R4698 T5423 T5421 pobj min,for
R4699 T5424 T5423 punct ",",min
R4700 T5425 T5426 nummod 35,cycles
R4701 T5426 T5423 appos cycles,min
R4702 T5427 T5401 prep at,using
R4703 T5428 T5429 nummod 94,°
R4704 T5429 T5427 pobj °,at
R4705 T5430 T5401 dobj C,using
R4706 T5431 T5401 prep for,using
R4707 T5432 T5433 nummod 45,s
R4708 T5433 T5431 pobj s,for
R4709 T5434 T5433 punct ",",s
R4710 T5435 T5437 nummod 52,C
R4711 T5436 T5437 compound °,C
R4712 T5437 T5433 appos C,s
R4713 T5438 T5401 prep for,using
R4714 T5439 T5438 pobj 45,for
R4715 T5440 T5401 dobj s,using
R4716 T5441 T5440 punct ",",s
R4717 T5442 T5444 nummod 72,C
R4718 T5443 T5444 compound °,C
R4719 T5444 T5440 appos C,s
R4720 T5445 T5401 prep for,using
R4721 T5446 T5447 nummod 45,s.
R4722 T5447 T5445 pobj s.,for
R4970 T5754 T5754 ROOT GR-GATA-3,GR-GATA-3
R4971 T5755 T5778 prep In,separated
R4972 T5756 T5758 compound Vitro,Assay
R4973 T5757 T5758 compound Competition,Assay
R4976 T5760 T5759 pobj Importin-α,for
R4977 T5761 T5760 punct (,Importin-α
R4978 T5762 T5763 compound Far-Western,ELISA
R4979 T5763 T5760 appos ELISA,Importin-α
R4980 T5764 T5760 punct ),Importin-α
R4981 T5765 T5766 compound Immunoprecipitated,importin-α
R4982 T5766 T5778 nsubjpass importin-α,separated
R4983 T5767 T5772 punct (,Biotechnology
R4984 T5768 T5772 npadvmod anti-importin-α,Biotechnology
R4985 T5769 T5772 punct ",",Biotechnology
R4986 T5770 T5771 compound Santa,Cruz
R4987 T5771 T5772 compound Cruz,Biotechnology
R4988 T5772 T5766 appos Biotechnology,importin-α
R4989 T5773 T5772 punct ),Biotechnology
R4990 T5774 T5772 prep from,Biotechnology
R4991 T5775 T5776 amod HuT-78,cells
R4992 T5776 T5774 pobj cells,from
R4993 T5777 T5778 auxpass was,separated
R4994 T5778 T5778 ROOT separated,separated
R4995 T5779 T5778 agent by,separated
R4996 T5780 T5779 pobj SDS-PAGE,by
R4997 T5781 T5778 cc and,separated
R4998 T5782 T5778 conj purified,separated
R4999 T5783 T5782 prep from,purified
R5000 T5784 T5786 det the,gel
R5001 T5785 T5786 amod excised,gel
R5002 T5786 T5783 pobj gel,from
R5003 T5787 T5786 prep by,gel
R5004 T5788 T5789 compound electroelution,[
R5005 T5789 T5787 pobj [,by
R5006 T5790 T5791 nummod 36,]
R5007 T5791 T5789 appos ],[
R5008 T5792 T5778 punct .,separated
R5009 T5793 T5796 advmod Similarly,was
R5010 T5794 T5796 punct ",",was
R5011 T5795 T5796 nsubj GATA-3,was
R5012 T5796 T5796 ROOT was,was
R5013 T5797 T5796 acomp isolated,was
R5014 T5798 T5797 prep from,isolated
R5015 T5799 T5801 amod nonstimulated,cells
R5016 T5800 T5801 amod HuT-78,cells
R5017 T5801 T5798 pobj cells,from
R5018 T5802 T5801 cc or,cells
R5019 T5803 T5801 conj cells,cells
R5020 T5804 T5796 conj stimulated,was
R5021 T5805 T5804 prep for,stimulated
R5022 T5806 T5807 nummod 30,min
R5023 T5807 T5805 pobj min,for
R5024 T5808 T5807 prep with,min
R5025 T5809 T5810 compound anti-CD3,/
R5026 T5810 T5808 pobj /,with
R5027 T5811 T5810 nummod CD28,/
R5028 T5812 T5810 cc and,/
R5029 T5813 T5814 nsubj GR,was
R5030 T5814 T5796 conj was,was
R5031 T5815 T5814 acomp isolated,was
R5032 T5816 T5815 prep from,isolated
R5033 T5817 T5816 pobj FP,from
R5034 T5818 T5817 punct (,FP
R5035 T5819 T5820 nummod 10,−
R5036 T5820 T5817 appos −,FP
R5037 T5821 T5822 nummod 8,M
R5038 T5822 T5817 conj M,FP
R5039 T5823 T5822 punct ",",M
R5040 T5824 T5825 nummod 30,min
R5041 T5825 T5822 appos min,M
R5042 T5826 T5822 punct ),M
R5043 T5827 T5814 conj stimulated,was
R5044 T5828 T5829 amod HuT-78,cells
R5045 T5829 T5827 dobj cells,stimulated
R5046 T5830 T5814 punct .,was
R5047 T5831 T5832 det These,proteins
R5048 T5832 T5835 nsubjpass proteins,refolded
R5049 T5833 T5835 auxpass were,refolded
R5050 T5834 T5835 advmod subsequently,refolded
R5051 T5835 T5835 ROOT refolded,refolded
R5052 T5836 T5835 prep in,refolded
R5053 T5837 T5838 amod glycine,solution
R5054 T5838 T5836 pobj solution,in
R5055 T5839 T5835 punct .,refolded
R5056 T5840 T5841 nummod 100,µl
R5057 T5841 T5852 nsubjpass µl,added
R5058 T5842 T5841 prep of,µl
R5059 T5843 T5844 amod importin-α,solution
R5060 T5844 T5842 pobj solution,of
R5061 T5845 T5844 punct (,solution
R5062 T5846 T5847 nummod 100,ng/ml
R5063 T5847 T5844 appos ng/ml,solution
R5064 T5848 T5847 prep in,ng/ml
R5065 T5849 T5848 pobj TBS,in
R5066 T5850 T5844 punct ),solution
R5067 T5851 T5852 auxpass was,added
R5068 T5852 T5852 ROOT added,added
R5069 T5853 T5852 prep to,added
R5071 T5855 T5854 dobj plates,96-well
R5072 T5856 T5855 acl coated,plates
R5073 T5857 T5856 prep with,coated
R5074 T5858 T5860 amod goat,antibody
R5075 T5859 T5860 amod anti-importin-α,antibody
R5085 T5869 T5870 nummod 100,ng/ml
R5086 T5870 T5867 appos ng/ml,GATA-3
R5087 T5871 T5870 prep in,ng/ml
R5088 T5872 T5871 pobj TBS,in
R5089 T5873 T5870 punct ),ng/ml
R5090 T5874 T5870 prep from,ng/ml
R5091 T5875 T5878 amod stimulated,cells
R5092 T5876 T5875 cc or,stimulated
R5093 T5877 T5875 conj unstimulated,stimulated
R5094 T5878 T5874 pobj cells,from
R5095 T5879 T5880 auxpass were,added
R5096 T5880 T5880 ROOT added,added
R5097 T5881 T5880 prep to,added
R5098 T5882 T5884 det the,wells
R5099 T5883 T5884 amod importin-α-coated,wells
R5100 T5884 T5881 pobj wells,to
R5101 T5885 T5880 punct .,added
R5102 T5886 T5901 nsubjpass GR,added
R5103 T5887 T5886 punct (,GR
R5104 T5888 T5891 nummod 10,ng/ml
R5105 T5889 T5888 cc or,10
R5106 T5890 T5888 conj 100,10
R5107 T5891 T5886 appos ng/ml,GR
R5108 T5892 T5891 prep in,ng/ml
R5109 T5893 T5892 pobj TBS,in
R5110 T5894 T5891 punct ),ng/ml
R5111 T5895 T5891 prep from,ng/ml
R5112 T5896 T5899 amod stimulated,cells
R5113 T5897 T5896 cc or,stimulated
R5114 T5898 T5896 conj unstimulated,stimulated
R5115 T5899 T5895 pobj cells,from
R5116 T5900 T5901 auxpass were,added
R5117 T5901 T5901 ROOT added,added
R5118 T5902 T5901 prep to,added
R5119 T5903 T5904 det some,wells
R5120 T5904 T5902 pobj wells,to
R5121 T5905 T5901 punct .,added
R5122 T5906 T5916 prep After,washed
R5123 T5907 T5911 det a,incubation
R5124 T5908 T5911 amod further,incubation
R5125 T5909 T5910 nummod 1,h
R5126 T5910 T5911 compound h,incubation
R5127 T5911 T5906 pobj incubation,After
R5128 T5912 T5916 punct ",",washed
R5129 T5913 T5914 det the,plate
R5130 T5914 T5916 nsubjpass plate,washed
R5131 T5915 T5916 auxpass was,washed
R5132 T5916 T5916 ROOT washed,washed
R5133 T5917 T5916 cc and,washed
R5134 T5918 T5916 conj incubated,washed
R5135 T5919 T5918 prep with,incubated
R5136 T5920 T5921 amod primary,antibodies
R5137 T5921 T5919 pobj antibodies,with
R5138 T5922 T5921 punct (,antibodies
R5139 T5923 T5924 det a,mixture
R5140 T5924 T5921 appos mixture,antibodies
R5141 T5925 T5924 prep of,mixture
R5142 T5926 T5927 compound rabbit,anti-GATA-3
R5143 T5927 T5925 pobj anti-GATA-3,of
R5144 T5928 T5927 cc and,anti-GATA-3
R5145 T5929 T5927 conj mouse,anti-GATA-3
R5146 T5930 T5927 conj anti-GR,anti-GATA-3
R5147 T5931 T5930 punct ",",anti-GR
R5148 T5932 T5934 compound Santa,Biotechnology
R5149 T5933 T5934 compound Cruz,Biotechnology
R5150 T5934 T5927 appos Biotechnology,anti-GATA-3
R5151 T5935 T5921 punct ),antibodies
R5152 T5936 T5918 prep for,incubated
R5153 T5937 T5938 nummod 1.5,h
R5154 T5938 T5936 pobj h,for
R5155 T5939 T5918 punct ",",incubated
R5156 T5940 T5918 cc and,incubated
R5157 T5941 T5942 advmod then,incubated
R5158 T5942 T5918 conj incubated,incubated
R5159 T5943 T5942 prep with,incubated
R5160 T5944 T5945 amod secondary,antibodies
R5161 T5945 T5943 pobj antibodies,with
R5162 T5946 T5916 punct .,washed
R5163 T5947 T5948 nummod FITC,swine
R5164 T5948 T5949 nsubj swine,anti-rabbit
R5165 T5949 T5959 nsubjpass anti-rabbit,used
R5166 T5950 T5949 dobj IgG,anti-rabbit
R5167 T5951 T5952 punct (,Dako
R5168 T5952 T5950 appos Dako,IgG
R5169 T5953 T5952 punct ",",Dako
R5170 T5954 T5952 npadvmod Cambridge,Dako
R5171 T5955 T5954 punct ",",Cambridge
R5172 T5956 T5954 appos UK,Cambridge
R5173 T5957 T5954 punct ),Cambridge
R5174 T5958 T5959 auxpass was,used
R5175 T5959 T5959 ROOT used,used
R5176 T5960 T5959 prep for,used
R5177 T5961 T5962 det the,detection
R5178 T5962 T5960 pobj detection,for
R5179 T5963 T5962 prep of,detection
R5207 T5991 T5993 nsubjpass levels,measured
R5208 T5992 T5993 auxpass were,measured
R5209 T5993 T5993 ROOT measured,measured
R5210 T5994 T5993 prep with,measured
R5211 T5995 T5998 det a,reader
R5212 T5996 T5998 amod fluorescent,reader
R5213 T5997 T5998 amod micro-plate,reader
R5214 T5998 T5994 pobj reader,with
R5215 T5999 T5998 punct (,reader
R5216 T6000 T5998 appos Bioline,reader
R5217 T6001 T6000 punct ",",Bioline
R5218 T6002 T6000 conj London,Bioline
R5219 T6003 T6002 punct ",",London
R5220 T6004 T6002 conj UK,London
R5221 T6005 T5998 punct ),reader
R5222 T6006 T5993 punct .,measured
R5304 T6161 T6163 compound NF-κB,NF-κB
R5305 T6162 T6163 compound Activation,NF-κB
R5306 T6163 T6170 nsubjpass NF-κB,determined
R5307 T6164 T6165 amod binding,activity
R5308 T6165 T6163 dobj activity,NF-κB
R5309 T6166 T6165 prep in,activity
R5310 T6167 T6168 amod nuclear,extracts
R5311 T6168 T6166 pobj extracts,in
R5312 T6169 T6170 auxpass was,determined
R5313 T6170 T6170 ROOT determined,determined
R5342 T6199 T6197 pobj plate,with
R5343 T6200 T6199 acl coated,plate
R5344 T6201 T6200 prep with,coated
R5345 T6202 T6204 det an,consensus
R5346 T6203 T6204 amod NF-κB,consensus
R5347 T6204 T6205 compound consensus,oligonucleotide
R5348 T6205 T6201 pobj oligonucleotide,with
R5349 T6206 T6196 punct .,incubated
R5350 T6207 T6209 nsubjpass Plates,washed
R5351 T6208 T6209 auxpass were,washed
R5352 T6209 T6209 ROOT washed,washed
R5353 T6210 T6209 prep before,washed
R5354 T6211 T6210 pobj addition,before
R5355 T6212 T6211 prep of,addition
R5356 T6213 T6215 det an,antibody
R5357 T6214 T6215 amod anti-p65,antibody
R5358 T6215 T6212 pobj antibody,of
R5359 T6216 T6209 punct .,washed
R5360 T6217 T6218 compound Antibody,binding
R5361 T6218 T6220 nsubjpass binding,detected
R5362 T6219 T6220 auxpass was,detected
R5363 T6220 T6220 ROOT detected,detected
R5364 T6221 T6220 prep with,detected
R5365 T6222 T6225 det a,antibody
R5366 T6223 T6225 amod secondary,antibody
R5367 T6224 T6225 amod HRP-conjugated,antibody
R5368 T6225 T6221 pobj antibody,with
R5369 T6226 T6220 cc and,detected
R5370 T6227 T6220 conj developed,detected
R5371 T6228 T6227 prep with,developed
R5372 T6229 T6230 compound TMB,substrate
R5373 T6230 T6228 pobj substrate,with
R5374 T6231 T6220 punct .,detected
R5375 T6232 T6233 det The,intensity
R5376 T6233 T6238 nsubjpass intensity,measured
R5377 T6234 T6233 prep of,intensity
R5378 T6235 T6236 det the,reaction
R5379 T6236 T6234 pobj reaction,of
R5380 T6237 T6238 auxpass was,measured
R5381 T6238 T6238 ROOT measured,measured
R5382 T6239 T6238 prep at,measured
R5383 T6240 T6241 nummod 450,nm
R5384 T6241 T6239 pobj nm,at
R5385 T6242 T6238 punct .,measured
R5594 T6486 T6488 compound Immunofluorescence,Immunofluorescence
R5595 T6487 T6488 compound Staining,Immunofluorescence
R5596 T6488 T6491 nsubjpass Immunofluorescence,performed
R5597 T6489 T6488 acl staining,Immunofluorescence
R5598 T6490 T6491 auxpass was,performed
R5599 T6491 T6491 ROOT performed,performed
R5600 T6492 T6494 mark as,described
R5601 T6493 T6494 advmod previously,described
R5602 T6494 T6491 advcl described,performed
R5603 T6495 T6497 nmod [,]
R5604 T6496 T6497 nummod 34,]
R5605 T6497 T6494 dobj ],described
R5606 T6498 T6491 punct .,performed
R5607 T6499 T6500 det All,staining
R5608 T6500 T6502 nsubjpass staining,performed
R5609 T6501 T6502 auxpass was,performed
R5610 T6502 T6502 ROOT performed,performed
R5611 T6503 T6502 prep at,performed
R5612 T6504 T6505 compound room,temperature
R5613 T6505 T6503 pobj temperature,at
R5614 T6506 T6503 cc and,at
R5615 T6507 T6503 conj under,at
R5616 T6508 T6507 pobj humidification,under
R5617 T6509 T6502 punct .,performed
R5618 T6510 T6512 nsubjpass Cells,collected
R5619 T6511 T6512 auxpass were,collected
R5620 T6512 T6512 ROOT collected,collected
R5621 T6513 T6512 cc and,collected
R5622 T6514 T6512 conj cytospins,collected
R5623 T6515 T6514 acl prepared,cytospins
R5624 T6516 T6515 prep in,prepared
R5625 T6517 T6518 det a,cytocentrifuge
R5626 T6518 T6516 pobj cytocentrifuge,in
R5627 T6519 T6521 punct (,II
R5628 T6520 T6521 compound Shandon,II
R5629 T6521 T6518 appos II,cytocentrifuge
R5630 T6522 T6521 punct ",",II
R5631 T6523 T6521 conj Shandon,II
R5632 T6524 T6523 punct ",",Shandon
R5633 T6525 T6523 conj Runcorn,Shandon
R5634 T6526 T6525 punct ",",Runcorn
R5635 T6527 T6525 conj UK,Runcorn
R5636 T6528 T6521 punct ),II
R5637 T6529 T6512 punct .,collected
R5638 T6530 T6532 nsubjpass Cells,permeabilized
R5639 T6531 T6532 auxpass were,permeabilized
R5640 T6532 T6532 ROOT permeabilized,permeabilized
R5641 T6533 T6532 punct ",",permeabilized
R5642 T6534 T6532 conj blocked,permeabilized
R5643 T6535 T6534 punct ",",blocked
R5644 T6536 T6532 cc and,permeabilized
R5645 T6537 T6538 advmod then,incubated
R5646 T6538 T6532 conj incubated,permeabilized
R5647 T6539 T6538 prep with,incubated
R5648 T6540 T6539 pobj GR,with
R5649 T6541 T6542 punct (,1
R5650 T6542 T6548 nummod 1,Biotechnology
R5651 T6543 T6548 nummod ∶,Biotechnology
R5652 T6544 T6545 nummod 50,E-20
R5653 T6545 T6548 compound E-20,Biotechnology
R5654 T6546 T6547 compound Santa,Cruz
R5655 T6547 T6548 compound Cruz,Biotechnology
R5656 T6548 T6540 appos Biotechnology,GR
R5657 T6549 T6538 punct ),incubated
R5658 T6550 T6538 cc or,incubated
R5659 T6551 T6538 conj anti-GATA-3,incubated
R5660 T6552 T6559 punct (,antibody
R5661 T6553 T6559 amod H-48,antibody
R5662 T6554 T6559 punct ;,antibody
R5663 T6555 T6556 compound Santa,Cruz
R5664 T6556 T6557 compound Cruz,Biotechnology
R5665 T6557 T6559 compound Biotechnology,antibody
R5666 T6558 T6557 punct ),Biotechnology
R5667 T6559 T6559 ROOT antibody,antibody
R5668 T6560 T6559 prep for,antibody
R5669 T6561 T6562 nummod 1,h
R5670 T6562 T6560 pobj h,for
R5671 T6563 T6562 prep at,h
R5672 T6564 T6565 compound room,temperature
R5673 T6565 T6563 pobj temperature,at
R5674 T6566 T6532 punct .,permeabilized
R5675 T6567 T6579 prep After,incubated
R5676 T6568 T6569 nummod three,washes
R5677 T6569 T6567 pobj washes,After
R5678 T6570 T6569 prep in,washes
R5679 T6571 T6572 amod phosphate-buffered,saline
R5680 T6572 T6570 pobj saline,in
R5681 T6573 T6574 punct (,PBS
R5682 T6574 T6569 appos PBS,washes
R5683 T6575 T6569 punct ),washes
R5684 T6576 T6579 punct ",",incubated
R5685 T6577 T6579 nsubjpass cells,incubated
R5686 T6578 T6579 auxpass were,incubated
R5687 T6579 T6579 ROOT incubated,incubated
R5688 T6580 T6579 prep with,incubated
R5689 T6581 T6582 amod tetrarhodamine,isothiocyanate
R5690 T6582 T6580 pobj isothiocyanate,with
R5691 T6583 T6586 amod conjugated,antibody
R5692 T6584 T6586 nmod goat,antibody
R5693 T6585 T6586 amod anti-rabbit,antibody
R5694 T6586 T6586 ROOT antibody,antibody
R5695 T6587 T6588 punct (,Dako
R5696 T6588 T6586 appos Dako,antibody
R5697 T6589 T6588 punct ),Dako
R5698 T6590 T6586 punct .,antibody
R5699 T6591 T6593 nsubjpass Cytospins,counterstained
R5700 T6592 T6593 auxpass were,counterstained
R5701 T6593 T6593 ROOT counterstained,counterstained
R5702 T6594 T6593 prep with,counterstained
R5703 T6595 T6596 nummod 4,′
R5704 T6596 T6600 nummod ′,dihydrochloride
R5705 T6597 T6599 punct ",6",diamidino-2-phenylindole
R5706 T6598 T6599 punct -,diamidino-2-phenylindole
R5707 T6599 T6600 compound diamidino-2-phenylindole,dihydrochloride
R5708 T6600 T6594 pobj dihydrochloride,with
R5709 T6601 T6600 punct (,dihydrochloride
R5710 T6602 T6600 appos DAPI,dihydrochloride
R5711 T6603 T6600 punct ),dihydrochloride
R5712 T6604 T6600 punct ",",dihydrochloride
R5713 T6605 T6611 det a,stain
R5714 T6606 T6611 amod fluorescent,stain
R5715 T6607 T6609 amod blue,indole
R5716 T6608 T6609 amod nuclear,indole
R5717 T6609 T6611 compound indole,stain
R5718 T6610 T6611 compound chromatin,stain
R5719 T6611 T6600 appos stain,dihydrochloride
R5720 T6612 T6593 punct ",",counterstained
R5721 T6613 T6593 cc and,counterstained
R5722 T6614 T6593 conj mounted,counterstained
R5723 T6615 T6614 prep in,mounted
R5724 T6616 T6615 pobj PBS,in
R5725 T6617 T6614 punct :,mounted
R5726 T6618 T6614 conj glycerol,mounted
R5727 T6619 T6618 punct (,glycerol
R5728 T6620 T6621 nummod 50,∶
R5729 T6621 T6618 appos ∶,glycerol
R5730 T6622 T6621 nummod 50,∶
R5731 T6623 T6618 punct ),glycerol
R5732 T6624 T6593 punct .,counterstained
R5733 T6625 T6627 det The,signal
R5734 T6626 T6627 amod immunopositive,signal
R5735 T6627 T6629 nsubjpass signal,characterised
R5736 T6628 T6629 auxpass was,characterised
R5737 T6629 T6629 ROOT characterised,characterised
R5738 T6630 T6629 advcl using,characterised
R5739 T6631 T6632 nmod laser,scanning
R5740 T6632 T6630 dobj scanning,using
R5741 T6633 T6634 amod confocal,microscopy
R5742 T6634 T6632 dobj microscopy,scanning
R5743 T6635 T6632 prep on,scanning
R5744 T6636 T6642 det a,cytometer
R5745 T6637 T6639 nmod Leica,NT/SP
R5746 T6638 T6639 compound TCS,NT/SP
R5747 T6639 T6642 nmod NT/SP,cytometer
R5748 T6640 T6642 amod interactive,cytometer
R5749 T6641 T6642 compound laser,cytometer
R5750 T6642 T6635 pobj cytometer,on
R5751 T6643 T6642 acl equipped,cytometer
R5752 T6644 T6643 prep with,equipped
R5753 T6645 T6646 compound confocal,optics
R5754 T6646 T6644 pobj optics,with
R5782 T6674 T6675 det the,primary
R5783 T6675 T6673 pobj primary,without
R5784 T6676 T6677 auxpass were,used
R5785 T6677 T6677 ROOT used,used
R5786 T6678 T6677 prep as,used
R5787 T6679 T6678 pobj controls,as
R5788 T6680 T6677 punct .,used
R5789 T6681 T6682 advmod Positively,stained
R5790 T6682 T6683 amod stained,nuclei
R5791 T6683 T6688 nsubjpass nuclei,counted
R5792 T6684 T6683 cc and,nuclei
R5793 T6685 T6686 amod total,cells
R5794 T6686 T6683 conj cells,nuclei
R5795 T6687 T6688 auxpass were,counted
R5796 T6688 T6688 ROOT counted,counted
R5797 T6689 T6688 punct (,counted
R5798 T6690 T6688 npadvmod 500,counted
R5799 T6691 T6688 punct ),counted
R5800 T6692 T6688 prep on,counted
R5801 T6693 T6694 det each,slide
R5802 T6694 T6692 pobj slide,on
R5803 T6695 T6694 prep with,slide
R5804 T6696 T6697 det the,observer
R5805 T6697 T6695 pobj observer,with
R5806 T6698 T6688 conj blinded,counted
R5807 T6699 T6698 prep to,blinded
R5808 T6700 T6701 det the,treatment
R5809 T6701 T6699 pobj treatment,to
R5810 T6702 T6688 punct .,counted
R5958 T6937 T6938 compound GATA-3-GFP,Construct
R5959 T6938 T6948 nsubjpass Construct,obtained
R5960 T6939 T6941 det The,clone
R5961 T6940 T6941 compound GATA-3,clone
R5962 T6941 T6938 appos clone,Construct
R5963 T6942 T6943 punct (,BC003070
R5964 T6943 T6941 appos BC003070,clone
R5965 T6944 T6941 punct ),clone
R5966 T6945 T6946 amod complete,cDNA
R5967 T6946 T6948 nsubjpass cDNA,obtained
R5968 T6947 T6948 auxpass was,obtained
R5969 T6948 T6948 ROOT obtained,obtained
R5970 T6949 T6948 prep from,obtained
R5971 T6950 T6952 compound Invitrogen,Technologies
R5972 T6951 T6952 compound Life,Technologies
R5973 T6952 T6949 pobj Technologies,from
R5974 T6953 T6948 prep as,obtained
R5975 T6954 T6962 det a,insert
R5976 T6955 T6958 nummod 5,EcoRI/3
R5977 T6956 T6958 punct ′,EcoRI/3
R5978 T6957 T6958 punct -,EcoRI/3
R5979 T6958 T6962 compound EcoRI/3,insert
R5980 T6959 T6961 punct ′,XhoI
R5981 T6960 T6961 punct -,XhoI
R5982 T6961 T6962 compound XhoI,insert
R5983 T6962 T6953 pobj insert,as
R5984 T6963 T6962 prep of,insert
R5985 T6964 T6963 pobj GATA-3,of
R5986 T6965 T6962 prep in,insert
R5987 T6966 T6968 det the,vector
R5988 T6967 T6968 compound pOTB7,vector
R5989 T6968 T6965 pobj vector,in
R5990 T6969 T6948 punct .,obtained
R5991 T6970 T6972 nsubjpass GATA-3,excised
R5992 T6971 T6972 auxpass was,excised
R5993 T6972 T6972 ROOT excised,excised
R5994 T6973 T6972 prep from,excised
R5995 T6974 T6973 pobj pOTB7,from
R5996 T6975 T6972 advcl using,excised
R5997 T6976 T6977 compound XhoI,digestion
R5998 T6977 T6975 dobj digestion,using
R5999 T6978 T6977 cc and,digestion
R6000 T6979 T6977 conj pEGFP-C2,digestion
R6001 T6980 T6981 punct (,Clontech
R6002 T6981 T6977 appos Clontech,digestion
R6003 T6982 T6981 punct ",",Clontech
R6004 T6983 T6981 conj Saint-Germain-en-Laye,Clontech
R6005 T6984 T6983 punct ",",Saint-Germain-en-Laye
R6006 T6985 T6983 conj France,Saint-Germain-en-Laye
R6007 T6986 T6981 punct ),Clontech
R6008 T6987 T6988 auxpass was,digested
R6009 T6988 T6972 advcl digested,excised
R6010 T6989 T6988 prep with,digested
R6011 T6990 T6989 pobj BamHI,with
R6012 T6991 T6972 punct .,excised
R6013 T6992 T6994 nsubjpass DNA,recovered
R6014 T6993 T6994 auxpass was,recovered
R6015 T6994 T6994 ROOT recovered,recovered
R6016 T6995 T6994 agent by,recovered
R6017 T6996 T6997 compound phenol,extraction
R6018 T6997 T6995 pobj extraction,by
R6019 T6998 T6997 cc and,extraction
R6020 T6999 T7000 compound ethanol,precipitation
R6021 T7000 T6997 conj precipitation,extraction
R6022 T7001 T6994 punct ",",recovered
R6023 T7002 T6994 cc and,recovered
R6024 T7003 T7006 preconj both,fragment
R6025 T7004 T7006 det the,fragment
R6026 T7005 T7006 compound GATA-3,fragment
R6027 T7006 T7029 nsubjpass fragment,inactivated
R6028 T7007 T7006 cc and,fragment
R6029 T7008 T7010 det the,vector
R6030 T7009 T7010 compound pEGFP-C2,vector
R6031 T7010 T7006 conj vector,fragment
R6032 T7011 T7006 acl blunt-ended,fragment
R6033 T7012 T7011 agent by,blunt-ended
R6034 T7013 T7012 pobj incubation,by
R6035 T7014 T7011 prep with,blunt-ended
R6036 T7015 T7014 pobj Klenow,with
R6037 T7016 T7015 punct (,Klenow
R6038 T7017 T7018 compound Bioline,Bio-27029
R6039 T7018 T7015 appos Bio-27029,Klenow
R6040 T7019 T7015 punct ),Klenow
R6041 T7020 T7011 prep for,blunt-ended
R6042 T7021 T7022 nummod 30,min
R6043 T7022 T7020 pobj min,for
R6044 T7023 T7022 prep at,min
R6045 T7024 T7025 nummod 37,°
R6046 T7025 T7027 nummod °,Klenow
R6047 T7026 T7027 compound C.,Klenow
R6048 T7027 T7023 pobj Klenow,at
R6049 T7028 T7029 auxpass was,inactivated
R6050 T7029 T6994 conj inactivated,recovered
R6051 T7030 T7029 agent by,inactivated
R6052 T7031 T7030 pobj incubation,by
R6053 T7032 T7029 prep for,inactivated
R6054 T7033 T7034 nummod 10,min
R6055 T7034 T7032 pobj min,for
R6056 T7035 T7034 prep at,min
R6057 T7036 T7037 nummod 75,°
R6058 T7037 T7039 nummod °,DNA
R6059 T7038 T7039 compound C.,DNA
R6060 T7039 T7035 pobj DNA,at
R6061 T7040 T7041 auxpass was,recovered
R6062 T7041 T6994 conj recovered,recovered
R6063 T7042 T7041 agent by,recovered
R6064 T7043 T7044 compound phenol,extraction
R6065 T7044 T7042 pobj extraction,by
R6066 T7045 T7044 cc and,extraction
R6067 T7046 T7047 compound ethanol,precipitation
R6068 T7047 T7044 conj precipitation,extraction
R6069 T7048 T7041 punct ",",recovered
R6070 T7049 T6994 cc and,recovered
R6071 T7050 T7054 preconj both,fragment
R6072 T7051 T7054 det the,fragment
R6073 T7052 T7054 amod blunt-ended,fragment
R6074 T7053 T7054 compound GATA-3,fragment
R6075 T7054 T7061 nsubjpass fragment,digested
R6076 T7055 T7054 cc and,fragment
R6077 T7056 T7058 det the,vector
R6078 T7057 T7058 compound GFP,vector
R6079 T7058 T7054 conj vector,fragment
R6080 T7059 T7061 auxpass were,digested
R6081 T7060 T7061 advmod subsequently,digested
R6082 T7061 T6994 conj digested,recovered
R6083 T7062 T7061 prep with,digested
R6084 T7063 T7062 pobj EcoRI,with
R6085 T7064 T7061 prep before,digested
R6086 T7065 T7070 det the,′
R6087 T7066 T7070 nummod 5,′
R6088 T7067 T7069 punct ′,EcoRI/3
R6089 T7068 T7069 punct -,EcoRI/3
R6090 T7069 T7070 compound EcoRI/3,′
R6091 T7070 T7064 pobj ′,before
R6092 T7071 T7074 amod blunt,fragment
R6093 T7072 T7074 compound end,fragment
R6094 T7073 T7074 compound GATA-3,fragment
R6095 T7074 T7076 nsubjpass fragment,inserted
R6096 T7075 T7076 auxpass was,inserted
R6097 T7076 T6994 conj inserted,recovered
R6098 T7077 T7076 prep into,inserted
R6099 T7078 T7083 det the,′
R6100 T7079 T7082 nummod 5,EcoRI/3
R6101 T7080 T7082 punct ′,EcoRI/3
R6247 T7294 T7286 appos serum,h
R6248 T7295 T7294 prep in,serum
R6249 T7296 T7295 pobj RPMI1640,in
R6250 T7297 T7298 nummod +15,L-glutamine
R6251 T7298 T7294 appos L-glutamine,serum
R6252 T7299 T7286 punct ),h
R6253 T7300 T7258 prep according,transfected
R6254 T7301 T7300 prep to,according
R6255 T7302 T7305 det the,protocol
R6256 T7303 T7305 amod general,protocol
R6257 T7304 T7305 compound Amaxa,protocol
R6258 T7305 T7301 pobj protocol,to
R6412 T7474 T7475 compound Microscopy,HuT-78
R6413 T7475 T7476 compound HuT-78,cells
R6414 T7476 T7480 nsubjpass cells,maintained
R6415 T7477 T7476 acl expressing,cells
R6416 T7478 T7477 dobj GATA-3-GFP,expressing
R6417 T7479 T7480 auxpass were,maintained
R6418 T7480 T7480 ROOT maintained,maintained
R6419 T7481 T7480 prep at,maintained
R6420 T7482 T7483 nummod 37,°
R6421 T7483 T7484 nummod °,C
R6422 T7484 T7481 pobj C,at
R6423 T7485 T7484 prep in,C
R6424 T7486 T7487 compound growth,medium
R6425 T7487 T7485 pobj medium,in
R6426 T7488 T7480 prep in,maintained
R6427 T7489 T7493 det a,chamber
R6428 T7490 T7493 amod closed,chamber
R6429 T7491 T7493 nummod FCS2,chamber
R6430 T7492 T7493 compound perfusion,chamber
R6431 T7493 T7488 pobj chamber,in
R6432 T7494 T7495 punct (,Bioptechs
R6433 T7495 T7493 appos Bioptechs,chamber
R6434 T7496 T7495 punct ",",Bioptechs
R6435 T7497 T7495 conj Butler,Bioptechs
R6436 T7498 T7497 punct ",",Butler
R6437 T7499 T7497 conj Pennsylvania,Butler
R6438 T7500 T7499 punct ",",Pennsylvania
R6439 T7501 T7499 conj USA,Pennsylvania
R6440 T7502 T7480 punct ),maintained
R6441 T7503 T7480 conj combined,maintained
R6442 T7504 T7503 prep with,combined
R6443 T7505 T7507 det an,heater
R6444 T7506 T7507 amod objective,heater
R6445 T7507 T7504 pobj heater,with
R6446 T7508 T7507 punct (,heater
R6447 T7509 T7507 appos Bioptechs,heater
R6448 T7510 T7507 punct ),heater
R6449 T7511 T7503 prep on,combined
R6450 T7512 T7513 det the,stage
R6451 T7513 T7511 pobj stage,on
R6452 T7514 T7518 det a,microscope
R6453 T7515 T7516 compound Zeiss,Axiovert
R6454 T7516 T7518 nmod Axiovert,microscope
R6455 T7517 T7518 nummod 200,microscope
R6456 T7518 T7503 npadvmod microscope,combined
R6457 T7519 T7520 punct (,Thornwood
R6458 T7520 T7518 appos Thornwood,microscope
R6459 T7521 T7520 punct ",",Thornwood
R6460 T7522 T7523 compound New,York
R6461 T7523 T7520 npadvmod York,Thornwood
R6462 T7524 T7523 punct ",",York
R6463 T7525 T7520 appos USA,Thornwood
R6464 T7526 T7520 punct ),Thornwood
R6465 T7527 T7480 punct .,maintained
R6466 T7528 T7530 nsubjpass Observations,made
R6467 T7529 T7530 auxpass were,made
R6468 T7530 T7530 ROOT made,made
R6469 T7531 T7530 agent by,made
R6470 T7532 T7534 nummod 40,1.0
R6471 T7533 T7534 nummod ×,1.0
R6472 T7534 T7531 pobj 1.0,by
R6473 T7535 T7538 nmod NA,lens
R6474 T7536 T7538 amod oil-immersion,lens
R6475 T7537 T7538 amod objective,lens
R6476 T7538 T7545 nmod lens,images
R6477 T7539 T7538 punct ",",lens
R6478 T7540 T7538 cc and,lens
R6479 T7541 T7538 conj fluorescence,lens
R6480 T7542 T7541 cc and,fluorescence
R6481 T7543 T7541 conj phase,fluorescence
R6482 T7544 T7545 compound contrast,images
R6483 T7545 T7547 nsubjpass images,gathered
R6484 T7546 T7547 auxpass were,gathered
R6485 T7547 T7530 conj gathered,made
R6486 T7548 T7547 advcl using,gathered
R6487 T7549 T7551 det a,ORCA-ER
R6488 T7550 T7551 compound Hamamatsu,ORCA-ER
R6489 T7551 T7548 dobj ORCA-ER,using
R6490 T7552 T7547 conj charged,gathered
R6491 T7553 T7555 amod coupled,camera
R6492 T7554 T7555 compound device,camera
R6493 T7555 T7552 dobj camera,charged
R6494 T7556 T7564 punct (,driven
R6495 T7557 T7564 dep Bridgewater,driven
R6496 T7558 T7557 punct ",",Bridgewater
R6497 T7559 T7560 compound New,Jersey
R6498 T7560 T7557 conj Jersey,Bridgewater
R6499 T7561 T7560 punct ",",Jersey
R6500 T7562 T7560 conj USA,Jersey
R6501 T7563 T7564 punct ),driven
R6502 T7564 T7552 advcl driven,charged
R6503 T7565 T7564 agent by,driven
R6504 T7566 T7567 compound Openlab,software
R6505 T7567 T7565 pobj software,by
R6506 T7568 T7569 punct (,Improvision
R6507 T7569 T7567 appos Improvision,software
R6508 T7570 T7569 punct ",",Improvision
R6509 T7571 T7569 npadvmod Coventry,Improvision
R6510 T7572 T7571 punct ",",Coventry
R6511 T7573 T7571 appos UK,Coventry
R6512 T7574 T7571 punct ),Coventry
R6513 T7575 T7547 punct .,gathered
R6514 T7576 T7578 nsubjpass Photographs,taken
R6515 T7577 T7578 auxpass were,taken
R6516 T7578 T7578 ROOT taken,taken
R6517 T7579 T7578 prep at,taken
R6518 T7580 T7579 pobj 0,at
R6519 T7581 T7580 punct ",",0
R6520 T7582 T7580 appos 30,0
R6521 T7583 T7580 punct ",",0
R6522 T7584 T7580 appos 60,0
R6523 T7585 T7584 punct ",",60
R6524 T7586 T7584 conj 120,60
R6525 T7587 T7578 punct ",",taken
R6526 T7588 T7578 cc and,taken
R6527 T7589 T7590 nummod 240,min
R6528 T7590 T7578 conj min,taken
R6529 T7591 T7578 punct .,taken
R6614 T7691 T7693 compound Densitometric,Densitometry
R6615 T7692 T7693 compound Analysis,Densitometry
R6616 T7693 T7698 nsubjpass Densitometry,performed
R6617 T7694 T7693 prep of,Densitometry
R6618 T7695 T7696 compound ECL,immunoblots
R6619 T7696 T7694 pobj immunoblots,of
R6620 T7697 T7698 auxpass was,performed
R6621 T7698 T7698 ROOT performed,performed
R6622 T7699 T7698 advcl using,performed
R6623 T7700 T7703 nmod Gelworks,software
R6624 T7701 T7703 nmod ID,software
R6625 T7702 T7703 amod intermediate,software
R6626 T7703 T7699 dobj software,using
R6627 T7704 T7706 punct (,Products
R6628 T7705 T7706 compound Ultraviolet,Products
R6629 T7706 T7703 appos Products,software
R6630 T7707 T7706 punct ",",Products
R6631 T7708 T7706 conj Cambridgeshire,Products
R6632 T7709 T7708 punct ",",Cambridgeshire
R6633 T7710 T7708 appos UK,Cambridgeshire
R6634 T7711 T7708 punct ),Cambridgeshire
R6635 T7712 T7698 punct .,performed
R6636 T7713 T7717 advmod Briefly,scanned
R6637 T7714 T7717 punct ",",scanned
R6638 T7715 T7717 nsubjpass immunoblots,scanned
R6639 T7716 T7717 auxpass were,scanned
R6640 T7717 T7717 ROOT scanned,scanned
R6641 T7718 T7717 cc and,scanned
R6642 T7719 T7721 nsubjpass gates,drawn
R6643 T7720 T7721 auxpass were,drawn
R6644 T7721 T7717 conj drawn,scanned
R6645 T7722 T7721 advmod tightly,drawn
R6646 T7723 T7721 prep around,drawn
R6647 T7724 T7725 det each,band
R6648 T7725 T7723 pobj band,around
R6649 T7726 T7721 punct .,drawn
R6650 T7727 T7728 compound Background,values
R6651 T7728 T7733 nsubjpass values,subtracted
R6652 T7729 T7728 prep from,values
R6653 T7730 T7731 det each,lane
R6654 T7731 T7729 pobj lane,from
R6655 T7732 T7733 auxpass were,subtracted
R6656 T7733 T7733 ROOT subtracted,subtracted
R6657 T7734 T7735 aux to,normalize
R6658 T7735 T7733 xcomp normalize,subtracted
R6659 T7736 T7737 det each,measurement
R6660 T7737 T7735 dobj measurement,normalize
R6661 T7738 T7733 punct .,subtracted
R6662 T7739 T7740 det The,bands
R6663 T7740 T7742 nsubjpass bands,quantified
R6664 T7741 T7742 auxpass were,quantified
R6665 T7742 T7742 ROOT quantified,quantified
R6666 T7743 T7742 advcl using,quantified
R6667 T7744 T7746 det the,software
R6668 T7745 T7746 compound Gelworks,software
R6669 T7746 T7743 dobj software,using
R6670 T7747 T7742 punct .,quantified
R6671 T7748 T7750 det All,blots
R6672 T7749 T7750 amod Western,blots
R6673 T7750 T7752 nsubjpass blots,exposed
R6674 T7751 T7752 auxpass were,exposed
R6675 T7752 T7752 ROOT exposed,exposed
R6676 T7753 T7752 prep to,exposed
R6677 T7754 T7753 pobj film,to
R6678 T7755 T7754 prep for,film
R6679 T7756 T7757 amod varying,lengths
R6680 T7757 T7755 pobj lengths,for
R6681 T7758 T7757 prep of,lengths
R6682 T7759 T7758 pobj time,of
R6683 T7760 T7752 punct ",",exposed
R6684 T7761 T7752 cc and,exposed
R6685 T7762 T7763 advmod only,films
R6686 T7763 T7770 nsubjpass films,selected
R6687 T7764 T7763 acl generating,films
R6688 T7765 T7766 amod subsaturating,levels
R6689 T7766 T7764 dobj levels,generating
R6690 T7767 T7766 prep of,levels
R6691 T7768 T7767 pobj intensity,of
R6692 T7769 T7770 auxpass were,selected
R6693 T7770 T7752 conj selected,exposed
R6694 T7771 T7770 prep for,selected
R6695 T7772 T7773 amod densitometric,evaluation
R6696 T7773 T7771 pobj evaluation,for
R6697 T7774 T7770 punct .,selected
R6852 T7934 T7935 compound Statistical,Analysis
R6853 T7935 T7936 compound Analysis,Data
R6854 T7936 T7944 nsubjpass Data,presented
R6855 T7937 T7936 prep from,Data
R6856 T7938 T7942 nummod three,experiments
R6857 T7939 T7938 cc or,three
R6858 T7940 T7938 conj more,three
R6859 T7941 T7942 amod independent,experiments
R6860 T7942 T7937 pobj experiments,from
R6861 T7943 T7944 auxpass are,presented
R6862 T7944 T7944 ROOT presented,presented
R6863 T7945 T7944 prep as,presented
R6864 T7946 T7950 det the,error
R6865 T7947 T7950 amod mean,error
R6866 T7948 T7950 compound ±,error
R6867 T7949 T7950 compound standard,error
R6868 T7950 T7945 pobj error,as
R6869 T7951 T7950 prep of,error
R6870 T7952 T7953 det the,mean
R6871 T7953 T7951 pobj mean,of
R6872 T7954 T7955 punct (,SEM
R6873 T7955 T7953 appos SEM,mean
R6874 T7956 T7955 punct ),SEM
R6875 T7957 T7950 punct ",",error
R6876 T7958 T7950 prep except,error
R6877 T7959 T7960 advmod where,stated
R6878 T7960 T7958 pcomp stated,except
R6879 T7961 T7944 cc and,presented
R6880 T7962 T7963 auxpass were,compared
R6881 T7963 T7944 conj compared,presented
R6882 T7964 T7963 advcl using,compared
R6930 T8012 T8009 dobj test,signed
R6931 T8013 T8009 punct .,signed
R6932 T8014 T8019 nsubjpass Data,presented
R6933 T8015 T8014 prep from,Data
R6934 T8016 T8017 det this,analysis
R6935 T8017 T8015 pobj analysis,from
R6936 T8018 T8019 auxpass are,presented
R6937 T8019 T8019 ROOT presented,presented
R6938 T8020 T8019 prep as,presented
R6939 T8021 T8023 det a,plot
R6940 T8022 T8023 compound box-and-whiskers,plot
R6941 T8023 T8020 pobj plot,as
R6942 T8024 T8019 punct .,presented
R6943 T8025 T8027 poss Friedman,test
R6944 T8026 T8025 case 's,Friedman
R6945 T8027 T8029 nsubjpass test,used
R6946 T8028 T8029 auxpass was,used
R6947 T8029 T8029 ROOT used,used
R6948 T8030 T8035 mark as,obtained
R6949 T8031 T8033 nummod three,measures
R6950 T8032 T8033 amod matched,measures
R6951 T8033 T8035 nsubjpass measures,obtained
R6952 T8034 T8035 auxpass were,obtained
R6953 T8035 T8029 advcl obtained,used
R6954 T8036 T8035 advcl using,obtained
R6955 T8037 T8036 dobj placebo,using
R6956 T8038 T8037 punct ",",placebo
R6957 T8039 T8040 nummod 100,µg
R6958 T8040 T8037 conj µg,placebo
R6959 T8041 T8040 punct ",",µg
R6960 T8042 T8040 cc and,µg
R6986 T8068 T8069 amod limited,numbers
R6987 T8069 T8065 pobj numbers,due
R6988 T8070 T8069 prep of,numbers
R6989 T8071 T8070 pobj participants,of
R6990 T8072 T8071 acl analysed,participants
R6991 T8073 T8061 punct (,distribution
R6992 T8074 T8061 appos seven,distribution
R6993 T8075 T8061 punct ),distribution
R6994 T8076 T8058 punct .,assume
R6995 T8077 T8079 det The,hypothesis
R6996 T8078 T8079 amod null,hypothesis
R6997 T8079 T8081 nsubjpass hypothesis,rejected
R6998 T8080 T8081 auxpass was,rejected
R6999 T8081 T8081 ROOT rejected,rejected
R7000 T8082 T8081 prep at,rejected
R7001 T8083 T8084 compound p,<
R7002 T8084 T8085 compound <,0.05
R7003 T8085 T8082 pobj 0.05,at
R7004 T8086 T8081 punct .,rejected
R3379 T3897 T3898 auxpass was,taken
R3380 T3898 T3886 advcl taken,given
R3381 T3899 T3898 prep for,taken
R3382 T3900 T3899 pobj preparation,for
R3383 T3901 T3900 prep of,preparation
R3384 T3902 T3901 pobj PBMCs,of
R3385 T3903 T3898 prep at,taken
R3386 T3904 T3903 pobj 1,at
R3387 T3905 T3898 cc and,taken
R3388 T3906 T3907 nummod 2,h
R3603 T4168 T4164 punct ),Oxford
R3717 T4282 T4284 nsubjpass reagents,purchased
R3718 T4283 T4284 auxpass were,purchased
R3719 T4284 T4284 ROOT purchased,purchased
R3720 T4285 T4284 prep from,purchased
R3721 T4286 T4285 pobj Sigma,from
R3722 T4287 T4288 punct (,Poole
R3723 T4288 T4284 npadvmod Poole,purchased
R3724 T4289 T4288 punct ",",Poole
R3725 T4290 T4288 appos UK,Poole
R3726 T4291 T4288 punct ),Poole
R4112 T4768 T4764 dobj virus,using
R4113 T4769 T4771 nmod RT,Promega
R4114 T4770 T4771 punct (,Promega
R4115 T4771 T4768 appos Promega,virus
R4116 T4772 T4771 punct ",",Promega
R4117 T4773 T4771 conj Southampton,Promega
R4118 T4774 T4773 punct ",",Southampton
R4119 T4775 T4773 conj UK,Southampton
R4120 T4776 T4771 punct ),Promega
R4121 T4777 T4763 punct .,transcribed
R4122 T4778 T4784 prep For,carried
R4123 T4779 T4780 amod relative,quantification
R4124 T4780 T4778 pobj quantification,For
R4125 T4781 T4784 punct ",",carried
R4126 T4782 T4784 nsubjpass RT-PCR,carried
R4127 T4783 T4784 auxpass was,carried
R4128 T4784 T4784 ROOT carried,carried
R4129 T4785 T4784 prt out,carried
R4130 T4786 T4784 advcl using,carried
R4131 T4787 T4788 compound cDNA,probes
R4132 T4788 T4786 dobj probes,using
R4133 T4789 T4784 punct .,carried
R4362 T5053 T5054 compound [,pH
R4363 T5054 T5048 appos pH,P-40
R4364 T5055 T5056 nummod 7.5,]
R4365 T5056 T5056 ROOT ],]
R4366 T5057 T5056 punct ",",]
R4367 T5058 T5060 nummod 150,NaCl
R4368 T5059 T5060 compound mM,NaCl
R4369 T5060 T5056 appos NaCl,]
R4370 T5061 T5056 punct ),]
R4371 T5062 T5056 prep in,]
R4372 T5063 T5064 det the,presence
R4373 T5064 T5062 pobj presence,in
R4374 T5065 T5064 prep of,presence
R4375 T5066 T5069 amod complete,inhibitor
R4376 T5067 T5069 compound protease,inhibitor
R4377 T5068 T5069 compound cocktail,inhibitor
R4378 T5069 T5065 pobj inhibitor,of
R4379 T5070 T5039 punct .,prepared
R4380 T5071 T5073 nsubjpass Lysates,centrifuged
R4381 T5072 T5073 auxpass were,centrifuged
R4382 T5073 T5073 ROOT centrifuged,centrifuged
R4383 T5074 T5073 prep at,centrifuged
R4384 T5075 T5076 nummod 4,°
R4385 T5076 T5074 pobj °,at
R4386 T5077 T5073 conj C,centrifuged
R4387 T5078 T5077 prep for,C
R4388 T5079 T5080 nummod 10,min
R4389 T5080 T5078 pobj min,for
R4390 T5081 T5080 prep at,min
R4391 T5082 T5083 nummod "12,000",rpm
R4392 T5083 T5081 pobj rpm,at
R4393 T5084 T5073 prep in,centrifuged
R4394 T5085 T5087 det an,microcentrifuge
R4395 T5086 T5087 compound Eppendorf,microcentrifuge
R4396 T5087 T5084 pobj microcentrifuge,in
R4397 T5088 T5089 aux to,remove
R4398 T5089 T5073 advcl remove,centrifuged
R4399 T5090 T5091 amod cellular,debris
R4400 T5091 T5089 dobj debris,remove
R4401 T5092 T5073 punct .,centrifuged
R4402 T5093 T5096 nsubjpass Samples,immunoprecipitated
R4403 T5094 T5096 auxpass were,immunoprecipitated
R4404 T5095 T5096 advmod then,immunoprecipitated
R4405 T5096 T5096 ROOT immunoprecipitated,immunoprecipitated
R4406 T5097 T5096 prep with,immunoprecipitated
R4407 T5098 T5100 preconj either,µl
R4408 T5099 T5100 nummod 10,µl
R4409 T5100 T5107 nsubj µl,using
R4410 T5101 T5100 prep of,µl
R4411 T5102 T5101 pobj antibody,of
R4412 T5103 T5102 prep against,antibody
R4413 T5104 T5103 pobj GATA-3,against
R4414 T5105 T5104 cc or,GATA-3
R4416 T5107 T5097 pcomp using,with
R4417 T5108 T5110 nmod A/G,slurry
R4418 T5109 T5110 amod agarose,slurry
R4419 T5110 T5107 dobj slurry,using
R4420 T5111 T5107 prep in,using
R4421 T5112 T5113 det the,presence
R4422 T5113 T5111 pobj presence,in
R4423 T5114 T5113 prep of,presence
R4424 T5115 T5116 amod protease,inhibitor
R4425 T5116 T5114 pobj inhibitor,of
R4426 T5117 T5107 advcl using,using
R4427 T5118 T5119 det the,Catch
R4428 T5119 T5117 dobj Catch,using
R4429 T5120 T5119 cc and,Catch
R4430 T5121 T5119 conj Release,Catch
R4431 T5122 T5119 conj methodology,Catch
R4432 T5123 T5125 punct (,Biotechnology
R4433 T5124 T5125 compound Upstate,Biotechnology
R4434 T5125 T5119 conj Biotechnology,Catch
R4435 T5126 T5125 punct ",",Biotechnology
R4436 T5127 T5128 compound Lake,Placid
R4437 T5128 T5125 conj Placid,Biotechnology
R4974 T5758 T5755 pobj Assay,In
R4975 T5759 T5758 prep for,Assay
R4668 T5393 T5395 punct ′,TCATGGCTCTGAAACGTTCTG-3
R4669 T5394 T5395 punct -,TCATGGCTCTGAAACGTTCTG-3
R4670 T5395 T5396 compound TCATGGCTCTGAAACGTTCTG-3,′
R4671 T5396 T5388 appos ′,′
R4672 T5397 T5368 punct .,detected
R4673 T5398 T5400 nsubjpass PCR,performed
R4674 T5399 T5400 auxpass was,performed
R4675 T5400 T5400 ROOT performed,performed
R4676 T5401 T5400 advcl using,performed
R4677 T5402 T5406 det a,cycler
R4678 T5403 T5404 nmod Hybaid,Omnigene
R4679 T5404 T5406 nmod Omnigene,cycler
R4680 T5405 T5406 amod thermal,cycler
R4681 T5406 T5401 dobj cycler,using
R4682 T5407 T5408 punct (,Hybaid
R4683 T5408 T5406 appos Hybaid,cycler
R4684 T5409 T5408 punct ",",Hybaid
R4685 T5410 T5408 npadvmod Ashford,Hybaid
R4686 T5411 T5410 punct ",",Ashford
R5070 T5854 T5853 pobj 96-well,to
R5076 T5860 T5857 pobj antibody,with
R5077 T5861 T5852 punct .,added
R5078 T5862 T5880 prep After,added
R5079 T5863 T5865 nummod 1,incubation
R5080 T5864 T5865 compound h,incubation
R5081 T5865 T5862 pobj incubation,After
R5082 T5866 T5880 punct ",",added
R5083 T5867 T5880 nsubjpass GATA-3,added
R5084 T5868 T5870 punct (,ng/ml
R5180 T5964 T5963 pobj GATA-3,of
R5181 T5965 T5959 punct ",",used
R5182 T5966 T5959 cc and,used
R5183 T5967 T5968 compound rhodamine,donkey
R5184 T5968 T5981 nsubjpass donkey,used
R5185 T5969 T5970 compound anti-mouse,IgG
R5186 T5970 T5981 nsubjpass IgG,used
R5187 T5971 T5972 punct (,Novus
R5188 T5972 T5970 appos Novus,IgG
R5189 T5973 T5972 punct ",",Novus
R5190 T5974 T5972 npadvmod Littleton,Novus
R5191 T5975 T5974 punct ",",Littleton
R5192 T5976 T5974 conj Colorado,Littleton
R5193 T5977 T5976 punct ",",Colorado
R5194 T5978 T5976 conj USA,Colorado
R5195 T5979 T5976 punct ),Colorado
R5196 T5980 T5981 auxpass was,used
R5197 T5981 T5959 conj used,used
R5198 T5982 T5981 prep for,used
R5199 T5983 T5984 det the,detection
R5200 T5984 T5982 pobj detection,for
R5201 T5985 T5984 prep of,detection
R5202 T5986 T5985 pobj GR,of
R5203 T5987 T5981 punct .,used
R5204 T5988 T5991 nmod FITC,levels
R5205 T5989 T5988 cc and,FITC
R5206 T5990 T5988 conj rhodamine,FITC
R5314 T6171 T6170 advcl using,determined
R5315 T6172 T6174 det an,kit
R5316 T6173 T6174 amod ELISA-based,kit
R5317 T6174 T6171 dobj kit,using
R5318 T6175 T6174 punct (,kit
R5319 T6176 T6177 amod Trans-AM,p65
R5320 T6177 T6174 appos p65,kit
R5321 T6178 T6177 punct ",",p65
R5322 T6179 T6180 compound Active,Motif
R5323 T6180 T6177 appos Motif,p65
R5324 T6181 T6180 punct ",",Motif
R5325 T6182 T6180 conj Rixensart,Motif
R5326 T6183 T6182 punct ",",Rixensart
R5327 T6184 T6182 conj Belgium,Rixensart
R5328 T6185 T6174 punct ),kit
R5329 T6186 T6170 punct .,determined
R5330 T6187 T6196 prep In,incubated
R5331 T6188 T6187 pobj brief,In
R5332 T6189 T6196 punct ",",incubated
R5333 T6190 T6191 nummod 5,µg
R5334 T6191 T6196 nsubjpass µg,incubated
R5335 T6192 T6191 prep of,µg
R5336 T6193 T6194 amod nuclear,extracts
R5337 T6194 T6192 pobj extracts,of
R5338 T6195 T6196 auxpass were,incubated
R5339 T6196 T6196 ROOT incubated,incubated
R5340 T6197 T6196 prep with,incubated
R5341 T6198 T6199 det a,plate
R5755 T6647 T6646 punct (,optics
R5756 T6648 T6649 compound Leica,Microsystems
R5757 T6649 T6646 appos Microsystems,optics
R5758 T6650 T6649 punct ",",Microsystems
R5759 T6651 T6649 conj Wetzlar,Microsystems
R5760 T6652 T6651 punct ",",Wetzlar
R5761 T6653 T6651 conj Germany,Wetzlar
R5762 T6654 T6646 punct ),optics
R5763 T6655 T6629 punct .,characterised
R5764 T6656 T6657 aux To,determine
R5765 T6657 T6677 advcl determine,used
R5766 T6658 T6659 det the,specificity
R5767 T6659 T6657 dobj specificity,determine
R5768 T6660 T6659 prep of,specificity
R5769 T6661 T6662 det the,antibodies
R5770 T6662 T6660 pobj antibodies,of
R5771 T6663 T6662 punct ",",antibodies
R5772 T6664 T6665 compound rabbit,serum
R5773 T6665 T6666 compound serum,immunoglobulin
R5774 T6666 T6659 conj immunoglobulin,specificity
R5775 T6667 T6666 punct (,immunoglobulin
R5776 T6668 T6666 appos Dako,immunoglobulin
R5777 T6669 T6666 punct ),immunoglobulin
R5778 T6670 T6666 cc and,immunoglobulin
R5779 T6671 T6672 amod secondary,antibodies
R5780 T6672 T6666 conj antibodies,immunoglobulin
R5781 T6673 T6657 prep without,determine
R6102 T7081 T7082 punct -,EcoRI/3
R6103 T7082 T7083 compound EcoRI/3,′
R6104 T7083 T7077 pobj ′,into
R6105 T7084 T7085 nsubj blunt,ended
R6106 T7085 T7076 advcl ended,inserted
R6107 T7086 T7087 compound GFP,vector
R6108 T7087 T7085 dobj vector,ended
R6109 T7088 T7085 punct .,ended
R6110 T7089 T7090 amod Positive,clones
R6111 T7090 T7092 nsubjpass clones,confirmed
R6112 T7091 T7092 auxpass were,confirmed
R6113 T7092 T7092 ROOT confirmed,confirmed
R6114 T7093 T7092 agent by,confirmed
R6115 T7094 T7093 pobj digestion,by
R6116 T7095 T7094 cc and,digestion
R6117 T7096 T7094 conj size,digestion
R6118 T7097 T7094 conj analysis,digestion
R6119 T7098 T7092 prep by,confirmed
R6120 T7099 T7100 nummod 1,%
R6121 T7100 T7103 compound %,electrophoresis
R6122 T7101 T7102 amod agarose,gel
R6123 T7102 T7103 compound gel,electrophoresis
R6124 T7103 T7098 pobj electrophoresis,by
R6125 T7104 T7098 cc and,by
R6126 T7105 T7098 conj by,by
R6127 T7106 T7105 pcomp sequencing,by
R6128 T7107 T7092 punct .,confirmed
R6207 T7254 T7255 compound Transfection,HuT-78
R6208 T7255 T7256 compound HuT-78,cells
R6209 T7256 T7258 nsubjpass cells,transfected
R6210 T7257 T7258 auxpass were,transfected
R6211 T7258 T7258 ROOT transfected,transfected
R6212 T7259 T7258 prep with,transfected
R6213 T7260 T7261 det either,EP8
R6214 T7261 T7259 pobj EP8,with
R6215 T7262 T7261 cc or,EP8
R6216 T7263 T7264 compound GFP,vector
R6217 T7264 T7261 conj vector,EP8
R6218 T7265 T7266 advmod only,DNA
R6219 T7266 T7267 nsubj DNA,using
R6220 T7267 T7258 advcl using,transfected
R6221 T7268 T7269 compound solution,R
R6222 T7269 T7267 dobj R,using
R6223 T7270 T7269 punct ",",R
R6224 T7271 T7272 compound programme,V-001
R6225 T7272 T7269 appos V-001,R
R6226 T7273 T7267 prep at,using
R6227 T7274 T7275 det a,ratio
R6228 T7275 T7282 nmod ratio,DNA
R6229 T7276 T7275 prep of,ratio
R6230 T7277 T7280 nummod 3,cells/4
R6231 T7278 T7280 nummod ×,cells/4
R6232 T7279 T7280 nummod 106,cells/4
R6233 T7280 T7276 pobj cells/4,of
R6234 T7281 T7282 compound µg,DNA
R6235 T7282 T7273 pobj DNA,at
R6236 T7283 T7267 prep for,using
R6237 T7284 T7283 pobj 7,for
R6238 T7285 T7286 nummod 8,h
R6239 T7286 T7284 appos h,7
R6240 T7287 T7286 prep in,h
R6241 T7288 T7289 amod complete,medium
R6242 T7289 T7287 pobj medium,in
R6243 T7290 T7286 punct (,h
R6244 T7291 T7292 nummod 10,%
R6245 T7292 T7294 nmod %,serum
R6246 T7293 T7294 amod bovine,serum
R6259 T7306 T7305 prep for,protocol
R6260 T7307 T7306 pobj nucleofection,for
R6261 T7308 T7258 punct .,transfected
R6262 T7309 T7310 det The,medium
R6263 T7310 T7313 nsubjpass medium,changed
R6264 T7311 T7313 auxpass was,changed
R6265 T7312 T7313 advmod subsequently,changed
R6266 T7313 T7313 ROOT changed,changed
R6267 T7314 T7313 prep to,changed
R6268 T7315 T7317 nummod 1,RPMI
R6269 T7316 T7315 quantmod %,1
R6270 T7317 T7314 pobj RPMI,to
R6271 T7318 T7313 prep for,changed
R6272 T7319 T7320 nummod 24,h
R6273 T7320 T7318 pobj h,for
R6274 T7321 T7325 mark before,added
R6275 T7322 T7323 amod transfected,cells
R6276 T7323 T7325 nsubjpass cells,added
R6277 T7324 T7325 auxpass were,added
R6278 T7325 T7313 advcl added,changed
R6279 T7326 T7325 prep to,added
R6280 T7327 T7336 amod anti-CD3,performed
R6281 T7328 T7331 nummod /,wells
R6282 T7329 T7331 nummod CD28,wells
R6283 T7330 T7331 amod treated,wells
R6284 T7331 T7327 dobj wells,anti-CD3
R6285 T7332 T7327 cc and,anti-CD3
R6286 T7333 T7335 amod live,videomicroscopy
R6287 T7334 T7335 compound cell,videomicroscopy
R6288 T7335 T7327 conj videomicroscopy,anti-CD3
R6289 T7336 T7326 pobj performed,to
R6290 T7337 T7313 punct .,changed
R6411 T7473 T7476 compound Time-Lapse,cells
R6883 T7965 T7966 compound GraphPad,Prism
R6884 T7966 T7964 dobj Prism,using
R6885 T7967 T7966 nummod 4,Prism
R6886 T7968 T7970 punct (,Software
R6887 T7969 T7970 compound GraphPad,Software
R6888 T7970 T7966 conj Software,Prism
R6889 T7971 T7970 punct ",",Software
R6890 T7972 T7970 appos http://www.graphpad.com,Software
R6891 T7973 T7970 punct ),Software
R6892 T7974 T7944 punct .,presented
R6893 T7975 T7976 nsubj Results,were
R6894 T7976 T7976 ROOT were,were
R6895 T7977 T7976 acomp analysed,were
R6896 T7978 T7976 advcl using,were
R6897 T7979 T7980 amod one-way,ANOVA
R6898 T7980 T7978 dobj ANOVA,using
R6899 T7981 T7978 prep with,using
R6900 T7982 T7984 compound Newman-Keuls,test
R6901 T7983 T7984 compound post,test
R6902 T7984 T7981 pobj test,with
R6903 T7985 T7978 prep except,using
R6904 T7986 T7985 prep for,except
R6905 T7987 T7988 det the,data
R6906 T7988 T7986 pobj data,for
R6907 T7989 T7988 prep from,data
R6908 T7990 T7995 det the,study
R6909 T7991 T7993 prep in,inhaled
R6910 T7992 T7991 pobj vivo,in
R6911 T7993 T7995 amod inhaled,study
R6912 T7994 T7995 compound FP,study
R6913 T7995 T7989 pobj study,from
R6914 T7996 T7995 punct ",",study
R6915 T7997 T7999 nsubjpass which,analysed
R6916 T7998 T7999 auxpass was,analysed
R6917 T7999 T7995 relcl analysed,study
R6918 T8000 T7999 agent by,analysed
R6919 T8001 T8003 poss Friedman,test
R6920 T8002 T8001 case 's,Friedman
R6921 T8003 T8000 pobj test,by
R6922 T8004 T8003 prep with,test
R6923 T8005 T8006 amod subsequent,Wilcoxson
R6924 T8006 T8004 pobj Wilcoxson,with
R6925 T8007 T8008 amod matched,pair
R6926 T8008 T8009 nsubj pair,signed
R6927 T8009 T7976 conj signed,were
R6928 T8010 T8012 compound rank,test
R6929 T8011 T8012 compound sum,test
R6961 T8043 T8044 nummod 500,µg
R6962 T8044 T8040 conj µg,µg
R6963 T8045 T8044 prep of,µg
R6964 T8046 T8047 amod inhaled,FP
R6965 T8047 T8045 pobj FP,of
R6966 T8048 T8047 punct ",",FP
R6967 T8049 T8050 nsubj which,had
R6968 T8050 T8047 relcl had,FP
R6969 T8051 T8053 amod variable,levels
R6970 T8052 T8053 compound baseline,levels
R6971 T8053 T8050 dobj levels,had
R6972 T8054 T8029 punct .,used
R6973 T8055 T8058 nsubj We,assume
R6974 T8056 T8058 aux did,assume
R6975 T8057 T8058 neg not,assume
R6976 T8058 T8058 ROOT assume,assume
R6977 T8059 T8061 det a,distribution
R6978 T8060 T8061 amod Gaussian,distribution
R6979 T8061 T8058 dobj distribution,assume
R6980 T8062 T8061 prep of,distribution
R6981 T8063 T8064 det the,data
R6982 T8064 T8062 pobj data,of
R6983 T8065 T8061 amod due,distribution
R6984 T8066 T8065 pcomp to,due
R6985 T8067 T8069 det the,numbers

UBERON-AE

Id Subject Object Predicate Lexical cue
T6271 7237-7242 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T7168 8588-8593 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T4298 1169-1172 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T4299 1499-1511 http://purl.obolibrary.org/obo/GO_0009293 denotes Transduction
T4599 2161-2173 http://purl.obolibrary.org/obo/GO_0051179 denotes localization
T4829 2239-2260 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse Transcription
T4830 2247-2260 http://purl.obolibrary.org/obo/GO_0006351 denotes Transcription
T4831 2295-2300 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T4832 2527-2529 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T4833 2587-2589 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T5179 2987-2992 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T5180 3214-3222 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T6713 6997-7003 http://purl.obolibrary.org/obo/GO_0023052 denotes signal
T7113 7665-7674 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion
T7114 8314-8323 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion
T7596 8930-8936 http://purl.obolibrary.org/obo/GO_0040007 denotes growth

GO-MF

Id Subject Object Predicate Lexical cue
T4300 854-864 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T4301 977-987 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T4302 1142-1152 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T4303 1374-1384 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T4304 1471-1479 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T4305 1169-1172 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T4834 2527-2529 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T4835 2587-2589 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T4836 2641-2645 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T5181 3289-3297 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T5452 3918-3932 http://purl.obolibrary.org/obo/GO_0003823 denotes immunoglobulin
T5453 4064-4068 http://purl.obolibrary.org/obo/GO_0005137 denotes IL-5
T6017 4956-4964 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6018 5267-5277 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T6019 5401-5411 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T6249 5723-5736 http://purl.obolibrary.org/obo/GO_0051059 denotes NF-κB binding
T6250 5729-5736 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T6251 6036-6043 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T6252 6017-6025 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6253 6089-6097 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6714 6607-6615 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6715 6784-6792 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6716 7218-7228 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T6717 7243-7257 http://purl.obolibrary.org/obo/GO_0003823 denotes immunoglobulin

GO-CC

Id Subject Object Predicate Lexical cue
T4306 854-864 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T4307 977-987 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T4308 1142-1152 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T4309 1374-1384 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T4310 1471-1479 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T4311 854-864 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T4312 977-987 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T4313 1142-1152 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T4314 1374-1384 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T4315 1471-1479 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T4316 1207-1211 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T5182 2800-2804 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T5183 2951-2955 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T5184 3289-3297 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T5185 3289-3297 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T5454 3727-3736 http://purl.obolibrary.org/obo/GO_0000785 denotes Chromatin
T5455 3757-3766 http://purl.obolibrary.org/obo/GO_0000785 denotes Chromatin
T5456 3847-3852 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6020 4510-4515 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6021 4657-4662 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6022 4666-4671 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6023 4776-4781 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6024 5046-5051 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6025 5155-5160 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6026 4956-4964 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6027 5267-5277 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T6028 5401-5411 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T6029 4956-4964 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6030 5267-5277 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T6031 5401-5411 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T6254 6017-6025 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6255 6089-6097 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6256 6017-6025 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6257 6089-6097 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6718 6607-6615 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6719 6784-6792 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6720 7218-7228 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T6721 7279-7289 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T6722 6607-6615 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6723 6784-6792 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6724 7218-7228 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T6725 7279-7289 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T6726 6700-6705 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6727 7369-7374 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6728 6924-6933 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T7341 8416-8421 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7342 8532-8537 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7343 8756-8761 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7344 8813-8817 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T7597 8875-8880 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7598 9283-9285 http://purl.obolibrary.org/obo/GO_0005783 denotes ER
T4600 1633-1637 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T4601 1742-1746 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell

sentences

Id Subject Object Predicate Lexical cue
T3670 23-52 Sentence denotes Participants and Study Design
T3671 53-250 Sentence denotes We studied patients with mild asthma who were not treated with inhaled corticosteroids who had been included in a previously reported double-blind, placebo-controlled, crossover study with FP [34].
T3672 251-500 Sentence denotes Seven patients with mild asthma entered the study and were randomized to receive a single inhalation of FP (100 and 500 µg) or a matched placebo control via a spacer chamber, and the other treatment was given after a wash-out period of at least 6 d.
T3673 501-581 Sentence denotes Blood was taken for preparation of PBMCs at 1 and 2 h after drug administration.
T3674 582-813 Sentence denotes All patients gave informed consent and the study was approved by the Ethics Committee of the Royal Brompton and Harefield Hospitals NHS Trust. The clinical study was conducted before the requirement for Clinical Trial Registration.
T3976 815-838 Sentence denotes Antibodies and Reagents
T3977 839-969 Sentence denotes The monoclonal antibodies against human CD3, CD28, GR, and importin-α were purchased from BD Biosciences (Oxford, United Kingdom).
T3978 970-1458 Sentence denotes Rabbit antibodies against human GATA-3 (H-48) and GR (E-20, sc-1003) were obtained from Santa Cruz Biotechnology (Santa Cruz, California, United States), polyclonal rabbit antibodies against phospho-p38 MAP kinase and phospho-ATF-2 from Cell Signaling Technology (New England Biolabs, Hertford, UK), monoclonal antibody against phosphoserine (clone 4H4) from Affiniti Research Products (Exeter, UK), and antibodies against rabbit IgG-conjugated TRITC from Dako Cytomation (Cambridge, UK).
T3979 1459-1573 Sentence denotes The anti-GR antibody (Clone 41) from BD Transduction Laboratories (Oxford, UK) was used for Western blot analysis.
T3980 1574-1631 Sentence denotes All other reagents were purchased from Sigma (Poole, UK).
T4615 2239-2264 Sentence denotes Reverse Transcription PCR
T4616 2265-2342 Sentence denotes Total RNA was extracted using lysis buffer (RNeasy kit; Qiagen, Crawley, UK).
T4617 2343-2438 Sentence denotes During RNA purification, genomic DNA was digested with RNase-free DNase (Amersham Biosciences).
T4618 2439-2557 Sentence denotes Next, 0.5 µg of total RNA was reversed transcribed using the avian myeloblastosis virus RT (Promega, Southampton, UK).
T4619 2558-2628 Sentence denotes For relative quantification, RT-PCR was carried out using cDNA probes.
T4620 2629-2686 Sentence denotes Primers for IL-4 were from Sigma-Genosys (Cambridge, UK).
T4621 2687-2798 Sentence denotes Sequences of GADPH used are as follows: forward 5′-CCACCCATGGCAAATTCCATGGC, reverse 3′-TCTAGACGGCAGGTCAGGTCCAC.
T4846 2800-2866 Sentence denotes Cell Fractionation, Immunoprecipitation, and Western Blot Analysis
T4847 2867-2944 Sentence denotes Nuclear and cytoplasmic fractions were prepared as previously described [35].
T4848 2945-3114 Sentence denotes Whole cell lysates were prepared in NP-40 lysis buffer (0.5% Nonidet P-40, 20 mM Tris-HCl [pH 7.5], 150 mM NaCl) in the presence of complete protease cocktail inhibitor.
T4849 3115-3230 Sentence denotes Lysates were centrifuged at 4°C for 10 min at 12,000 rpm in an Eppendorf microcentrifuge to remove cellular debris.
T4850 3231-3482 Sentence denotes Samples were then immunoprecipitated with either 10 µl of antibody against GATA-3 or importin-α using A/G agarose slurry in the presence of protease inhibitor using the Catch and Release methodology (Upstate Biotechnology, Lake Placid, New York, USA).
T4851 3483-3619 Sentence denotes Western blot analysis was performed using anti-GATA-3, anti-importin-α, anti-GR, anti-p-p38 MAP kinase, anti-p-ATF-2, and anti-p-serine.
T4852 3620-3725 Sentence denotes Immunoreactive proteins were detected using an enhanced chemiluminescence ECL kit (Amersham Biosciences).
T5202 3727-3756 Sentence denotes Chromatin Immunoprecipitation
T5203 3757-4140 Sentence denotes Chromatin immunoprecipitation (IP) was performed as previously described [12] in HuT-78 T-cells with 2 µg of anti-GATA-3 (Santa Cruz Biotechnology), or isotypic immunoglobulin G as a non-specific control (Santa Cruz Biotechnology) overnight at 4°C. Promoter sequences were detected with PCR primers for the IL-5 promoter (−445 to +4): forward 5′-TTAATCTAGCCACAGTCATAG-3′ and reverse:
T5204 4141-4169 Sentence denotes 5′-TCATGGCTCTGAAACGTTCTG-3′.
T5205 4170-4350 Sentence denotes PCR was performed using a Hybaid Omnigene thermal cycler (Hybaid, Ashford, UK) with cycling parameters of 72°C for 10 min, 35 cycles at 94°C for 45 s, 52°C for 45 s, 72°C for 45 s.
T5489 4352-4423 Sentence denotes GR-GATA-3 In Vitro Competition Assay for Importin-α (Far-Western ELISA)
T5490 4424-4599 Sentence denotes Immunoprecipitated importin-α (anti-importin-α, Santa Cruz Biotechnology) from HuT-78 cells was separated by SDS-PAGE and purified from the excised gel by electroelution [36].
T5491 4600-4782 Sentence denotes Similarly, GATA-3 was isolated from nonstimulated HuT-78 cells or cells stimulated for 30 min with anti-CD3/CD28 and GR was isolated from FP (10−8 M, 30 min) stimulated HuT-78 cells.
T5492 4783-4845 Sentence denotes These proteins were subsequently refolded in glycine solution.
T5493 4846-4965 Sentence denotes 100 µl of importin-α solution (100 ng/ml in TBS) was added to 96-well plates coated with goat anti-importin-α antibody.
T5494 4966-5094 Sentence denotes After 1 h incubation, GATA-3 (100 ng/ml in TBS) from stimulated or unstimulated cells were added to the importin-α-coated wells.
T5495 5095-5186 Sentence denotes GR (10 or 100 ng/ml in TBS) from stimulated or unstimulated cells were added to some wells.
T5496 5187-5412 Sentence denotes After a further 1 h incubation, the plate was washed and incubated with primary antibodies (a mixture of rabbit anti-GATA-3 and mouse anti-GR, Santa Cruz Biotechnology) for 1.5 h, and then incubated with secondary antibodies.
T5497 5413-5603 Sentence denotes FITC swine anti-rabbit IgG (Dako, Cambridge, UK) was used for the detection of GATA-3, and rhodamine donkey anti-mouse IgG (Novus, Littleton, Colorado, USA) was used for the detection of GR.
T5498 5604-5704 Sentence denotes FITC and rhodamine levels were measured with a fluorescent micro-plate reader (Bioline, London, UK).
T6074 5706-5722 Sentence denotes NF-κB Activation
T6075 5723-5855 Sentence denotes NF-κB binding activity in nuclear extracts was determined using an ELISA-based kit (Trans-AM p65, Active Motif, Rixensart, Belgium).
T6076 5856-5966 Sentence denotes In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide.
T6077 5967-6026 Sentence denotes Plates were washed before addition of an anti-p65 antibody.
T6078 6027-6131 Sentence denotes Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate.
T6079 6132-6185 Sentence denotes The intensity of the reaction was measured at 450 nm.
T6273 6187-6214 Sentence denotes Immunofluorescence Staining
T6274 6215-6286 Sentence denotes Immunofluorescence staining was performed as previously described [34].
T6275 6287-6359 Sentence denotes All staining was performed at room temperature and under humidification.
T6276 6360-6459 Sentence denotes Cells were collected and cytospins prepared in a cytocentrifuge (Shandon II, Shandon, Runcorn, UK).
T6277 6460-6644 Sentence denotes Cells were permeabilized, blocked, and then incubated with GR (1∶50 E-20 Santa Cruz Biotechnology) or anti-GATA-3 (H-48; Santa Cruz Biotechnology) antibody for 1 h at room temperature.
T6278 6645-6800 Sentence denotes After three washes in phosphate-buffered saline (PBS), cells were incubated with tetrarhodamine isothiocyanate–conjugated goat anti-rabbit antibody (Dako).
T6279 6801-6977 Sentence denotes Cytospins were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI), a fluorescent blue nuclear indole chromatin stain, and mounted in PBS:glycerol (50∶50).
T6280 6978-7181 Sentence denotes The immunopositive signal was characterised using laser scanning confocal microscopy on a Leica TCS NT/SP interactive laser cytometer equipped with confocal optics (Leica Microsystems, Wetzlar, Germany).
T6281 7182-7332 Sentence denotes To determine the specificity of the antibodies, rabbit serum immunoglobulin (Dako) and secondary antibodies without the primary were used as controls.
T6282 7333-7451 Sentence denotes Positively stained nuclei and total cells were counted (500) on each slide with the observer blinded to the treatment.
T7169 8396-8408 Sentence denotes Transfection
T7170 8409-8680 Sentence denotes HuT-78 cells were transfected with either EP8 or GFP vector only DNA using solution R, programme V-001 at a ratio of 3×106 cells/4 µg DNA for 7–8 h in complete medium (10% bovine serum in RPMI1640+15 L-glutamine) according to the general Amaxa protocol for nucleofection.
T7171 8681-8844 Sentence denotes The medium was subsequently changed to 1% RPMI for 24 h before transfected cells were added to anti-CD3/CD28 treated wells and live cell videomicroscopy performed.
T7353 8846-8867 Sentence denotes Time-Lapse Microscopy
T7354 8868-9136 Sentence denotes HuT-78 cells expressing GATA-3-GFP were maintained at 37°C in growth medium in a closed FCS2 perfusion chamber (Bioptechs, Butler, Pennsylvania, USA) combined with an objective heater (Bioptechs) on the stage a Zeiss Axiovert 200 microscope (Thornwood, New York, USA).
T7355 9137-9402 Sentence denotes Observations were made by 40×1.0 NA oil-immersion objective lens, and fluorescence and phase contrast images were gathered using a Hamamatsu ORCA-ER charged coupled device camera (Bridgewater, New Jersey, USA) driven by Openlab software (Improvision, Coventry, UK).
T7356 9403-9457 Sentence denotes Photographs were taken at 0, 30, 60, 120, and 240 min.
T7605 9459-9481 Sentence denotes Densitometric Analysis
T7606 9482-9611 Sentence denotes Densitometry of ECL immunoblots was performed using Gelworks ID intermediate software (Ultraviolet Products, Cambridgeshire, UK).
T7607 9612-9692 Sentence denotes Briefly, immunoblots were scanned and gates were drawn tightly around each band.
T7608 9693-9772 Sentence denotes Background values from each lane were subtracted to normalize each measurement.
T7609 9773-9827 Sentence denotes The bands were quantified using the Gelworks software.
T7610 9828-9999 Sentence denotes All Western blots were exposed to film for varying lengths of time, and only films generating subsaturating levels of intensity were selected for densitometric evaluation.
T7781 10001-10021 Sentence denotes Statistical Analysis
T7782 10022-10236 Sentence denotes Data from three or more independent experiments are presented as the mean±standard error of the mean (SEM), except where stated and were compared using GraphPad Prism 4 (GraphPad Software, http://www.graphpad.com).
T7783 10237-10460 Sentence denotes Results were analysed using one-way ANOVA with Newman-Keuls post test except for the data from the in vivo inhaled FP study, which was analysed by Friedman's test with subsequent Wilcoxson matched pair signed rank sum test.
T7784 10461-10526 Sentence denotes Data from this analysis are presented as a box-and-whiskers plot.
T7785 10527-10676 Sentence denotes Friedman's test was used as three matched measures were obtained using placebo, 100 µg, and 500 µg of inhaled FP, which had variable baseline levels.
T7786 10677-10834 Sentence denotes We did not assume a Gaussian distribution of the data due to the limited numbers of participants analysed (seven).The null hypothesis was rejected at p<0.05.
T4349 1633-1664 Sentence denotes Cell Culture and PBMC Isolation
T4350 1665-1814 Sentence denotes A human T cell line (HuT-78) was purchased from ECACC European Collection of Cell Culture (Wiltshire, UK) were cultured as previously described [12].
T4351 1815-1968 Sentence denotes PBMCs were isolated by density centrifugation over Ficoll-Hypaque (density, 1.077 g/ml; Amersham Biosciences, Amersham, UK) as previously described [34].
T4352 1969-2125 Sentence denotes Cells were stimulated with anti-CD3/CD28 (1 µg/ml each) for 1 h at 37°C to stimulate Th2 cytokine release in the presence or absence of FP (10−12 to 10−8M).
T4353 2126-2237 Sentence denotes Cytospins were prepared and GATA-3 localization determined by confocal microscopy as previously described [12].
T6774 7453-7473 Sentence denotes GATA-3-GFP Construct
T6775 7474-7623 Sentence denotes The GATA-3 clone (BC003070) complete cDNA was obtained from Invitrogen Life Technologies as a 5′- EcoRI/3′-XhoI insert of GATA-3 in the pOTB7 vector.
T6776 7624-7754 Sentence denotes GATA-3 was excised from pOTB7 using XhoI digestion and pEGFP-C2 (Clontech, Saint-Germain-en-Laye, France) was digested with BamHI.
T6777 7755-8279 Sentence denotes DNA was recovered by phenol extraction and ethanol precipitation, and both the GATA-3 fragment and the pEGFP-C2 vector blunt-ended by incubation with Klenow (Bioline Bio-27029) for 30 min at 37°C. Klenow was inactivated by incubation for 10 min at 75°C. DNA was recovered by phenol extraction and ethanol precipitation, and both the blunt-ended GATA-3 fragment and the GFP vector were subsequently digested with EcoRI before the 5′-EcoRI/3′–blunt end GATA-3 fragment was inserted into the 5′-EcoRI/3′–blunt ended GFP vector.
T6778 8280-8394 Sentence denotes Positive clones were confirmed by digestion and size analysis by 1% agarose gel electrophoresis and by sequencing.
T78 0-21 Sentence denotes Materials and Methods
T79 23-52 Sentence denotes Participants and Study Design
T80 53-250 Sentence denotes We studied patients with mild asthma who were not treated with inhaled corticosteroids who had been included in a previously reported double-blind, placebo-controlled, crossover study with FP [34].
T81 251-500 Sentence denotes Seven patients with mild asthma entered the study and were randomized to receive a single inhalation of FP (100 and 500 µg) or a matched placebo control via a spacer chamber, and the other treatment was given after a wash-out period of at least 6 d.
T82 501-581 Sentence denotes Blood was taken for preparation of PBMCs at 1 and 2 h after drug administration.
T83 582-724 Sentence denotes All patients gave informed consent and the study was approved by the Ethics Committee of the Royal Brompton and Harefield Hospitals NHS Trust.
T84 725-813 Sentence denotes The clinical study was conducted before the requirement for Clinical Trial Registration.
T85 815-838 Sentence denotes Antibodies and Reagents
T86 839-969 Sentence denotes The monoclonal antibodies against human CD3, CD28, GR, and importin-α were purchased from BD Biosciences (Oxford, United Kingdom).
T87 970-1458 Sentence denotes Rabbit antibodies against human GATA-3 (H-48) and GR (E-20, sc-1003) were obtained from Santa Cruz Biotechnology (Santa Cruz, California, United States), polyclonal rabbit antibodies against phospho-p38 MAP kinase and phospho-ATF-2 from Cell Signaling Technology (New England Biolabs, Hertford, UK), monoclonal antibody against phosphoserine (clone 4H4) from Affiniti Research Products (Exeter, UK), and antibodies against rabbit IgG-conjugated TRITC from Dako Cytomation (Cambridge, UK).
T88 1459-1573 Sentence denotes The anti-GR antibody (Clone 41) from BD Transduction Laboratories (Oxford, UK) was used for Western blot analysis.
T89 1574-1631 Sentence denotes All other reagents were purchased from Sigma (Poole, UK).
T90 1633-1664 Sentence denotes Cell Culture and PBMC Isolation
T91 1665-1814 Sentence denotes A human T cell line (HuT-78) was purchased from ECACC European Collection of Cell Culture (Wiltshire, UK) were cultured as previously described [12].
T92 1815-1968 Sentence denotes PBMCs were isolated by density centrifugation over Ficoll-Hypaque (density, 1.077 g/ml; Amersham Biosciences, Amersham, UK) as previously described [34].
T93 1969-2125 Sentence denotes Cells were stimulated with anti-CD3/CD28 (1 µg/ml each) for 1 h at 37°C to stimulate Th2 cytokine release in the presence or absence of FP (10−12 to 10−8M).
T94 2126-2237 Sentence denotes Cytospins were prepared and GATA-3 localization determined by confocal microscopy as previously described [12].
T95 2239-2264 Sentence denotes Reverse Transcription PCR
T96 2265-2342 Sentence denotes Total RNA was extracted using lysis buffer (RNeasy kit; Qiagen, Crawley, UK).
T97 2343-2438 Sentence denotes During RNA purification, genomic DNA was digested with RNase-free DNase (Amersham Biosciences).
T98 2439-2557 Sentence denotes Next, 0.5 µg of total RNA was reversed transcribed using the avian myeloblastosis virus RT (Promega, Southampton, UK).
T99 2558-2628 Sentence denotes For relative quantification, RT-PCR was carried out using cDNA probes.
T100 2629-2686 Sentence denotes Primers for IL-4 were from Sigma-Genosys (Cambridge, UK).
T101 2687-2798 Sentence denotes Sequences of GADPH used are as follows: forward 5′-CCACCCATGGCAAATTCCATGGC, reverse 3′-TCTAGACGGCAGGTCAGGTCCAC.
T102 2800-2866 Sentence denotes Cell Fractionation, Immunoprecipitation, and Western Blot Analysis
T103 2867-2944 Sentence denotes Nuclear and cytoplasmic fractions were prepared as previously described [35].
T104 2945-3114 Sentence denotes Whole cell lysates were prepared in NP-40 lysis buffer (0.5% Nonidet P-40, 20 mM Tris-HCl [pH 7.5], 150 mM NaCl) in the presence of complete protease cocktail inhibitor.
T105 3115-3230 Sentence denotes Lysates were centrifuged at 4°C for 10 min at 12,000 rpm in an Eppendorf microcentrifuge to remove cellular debris.
T106 3231-3482 Sentence denotes Samples were then immunoprecipitated with either 10 µl of antibody against GATA-3 or importin-α using A/G agarose slurry in the presence of protease inhibitor using the Catch and Release methodology (Upstate Biotechnology, Lake Placid, New York, USA).
T107 3483-3619 Sentence denotes Western blot analysis was performed using anti-GATA-3, anti-importin-α, anti-GR, anti-p-p38 MAP kinase, anti-p-ATF-2, and anti-p-serine.
T108 3620-3725 Sentence denotes Immunoreactive proteins were detected using an enhanced chemiluminescence ECL kit (Amersham Biosciences).
T109 3727-3756 Sentence denotes Chromatin Immunoprecipitation
T110 3757-4005 Sentence denotes Chromatin immunoprecipitation (IP) was performed as previously described [12] in HuT-78 T-cells with 2 µg of anti-GATA-3 (Santa Cruz Biotechnology), or isotypic immunoglobulin G as a non-specific control (Santa Cruz Biotechnology) overnight at 4°C.
T111 4006-4140 Sentence denotes Promoter sequences were detected with PCR primers for the IL-5 promoter (−445 to +4): forward 5′-TTAATCTAGCCACAGTCATAG-3′ and reverse:
T112 4141-4169 Sentence denotes 5′-TCATGGCTCTGAAACGTTCTG-3′.
T113 4170-4350 Sentence denotes PCR was performed using a Hybaid Omnigene thermal cycler (Hybaid, Ashford, UK) with cycling parameters of 72°C for 10 min, 35 cycles at 94°C for 45 s, 52°C for 45 s, 72°C for 45 s.
T114 4352-4423 Sentence denotes GR-GATA-3 In Vitro Competition Assay for Importin-α (Far-Western ELISA)
T115 4424-4599 Sentence denotes Immunoprecipitated importin-α (anti-importin-α, Santa Cruz Biotechnology) from HuT-78 cells was separated by SDS-PAGE and purified from the excised gel by electroelution [36].
T116 4600-4782 Sentence denotes Similarly, GATA-3 was isolated from nonstimulated HuT-78 cells or cells stimulated for 30 min with anti-CD3/CD28 and GR was isolated from FP (10−8 M, 30 min) stimulated HuT-78 cells.
T117 4783-4845 Sentence denotes These proteins were subsequently refolded in glycine solution.
T118 4846-4965 Sentence denotes 100 µl of importin-α solution (100 ng/ml in TBS) was added to 96-well plates coated with goat anti-importin-α antibody.
T119 4966-5094 Sentence denotes After 1 h incubation, GATA-3 (100 ng/ml in TBS) from stimulated or unstimulated cells were added to the importin-α-coated wells.
T120 5095-5186 Sentence denotes GR (10 or 100 ng/ml in TBS) from stimulated or unstimulated cells were added to some wells.
T121 5187-5412 Sentence denotes After a further 1 h incubation, the plate was washed and incubated with primary antibodies (a mixture of rabbit anti-GATA-3 and mouse anti-GR, Santa Cruz Biotechnology) for 1.5 h, and then incubated with secondary antibodies.
T122 5413-5603 Sentence denotes FITC swine anti-rabbit IgG (Dako, Cambridge, UK) was used for the detection of GATA-3, and rhodamine donkey anti-mouse IgG (Novus, Littleton, Colorado, USA) was used for the detection of GR.
T123 5604-5704 Sentence denotes FITC and rhodamine levels were measured with a fluorescent micro-plate reader (Bioline, London, UK).
T124 5706-5722 Sentence denotes NF-κB Activation
T125 5723-5855 Sentence denotes NF-κB binding activity in nuclear extracts was determined using an ELISA-based kit (Trans-AM p65, Active Motif, Rixensart, Belgium).
T126 5856-5966 Sentence denotes In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide.
T127 5967-6026 Sentence denotes Plates were washed before addition of an anti-p65 antibody.
T128 6027-6131 Sentence denotes Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate.
T129 6132-6185 Sentence denotes The intensity of the reaction was measured at 450 nm.
T130 6187-6214 Sentence denotes Immunofluorescence Staining
T131 6215-6286 Sentence denotes Immunofluorescence staining was performed as previously described [34].
T132 6287-6359 Sentence denotes All staining was performed at room temperature and under humidification.
T133 6360-6459 Sentence denotes Cells were collected and cytospins prepared in a cytocentrifuge (Shandon II, Shandon, Runcorn, UK).
T134 6460-6644 Sentence denotes Cells were permeabilized, blocked, and then incubated with GR (1∶50 E-20 Santa Cruz Biotechnology) or anti-GATA-3 (H-48; Santa Cruz Biotechnology) antibody for 1 h at room temperature.
T135 6645-6800 Sentence denotes After three washes in phosphate-buffered saline (PBS), cells were incubated with tetrarhodamine isothiocyanate–conjugated goat anti-rabbit antibody (Dako).
T136 6801-6977 Sentence denotes Cytospins were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI), a fluorescent blue nuclear indole chromatin stain, and mounted in PBS:glycerol (50∶50).
T137 6978-7181 Sentence denotes The immunopositive signal was characterised using laser scanning confocal microscopy on a Leica TCS NT/SP interactive laser cytometer equipped with confocal optics (Leica Microsystems, Wetzlar, Germany).
T138 7182-7332 Sentence denotes To determine the specificity of the antibodies, rabbit serum immunoglobulin (Dako) and secondary antibodies without the primary were used as controls.
T139 7333-7451 Sentence denotes Positively stained nuclei and total cells were counted (500) on each slide with the observer blinded to the treatment.
T140 7453-7473 Sentence denotes GATA-3-GFP Construct
T141 7474-7623 Sentence denotes The GATA-3 clone (BC003070) complete cDNA was obtained from Invitrogen Life Technologies as a 5′- EcoRI/3′-XhoI insert of GATA-3 in the pOTB7 vector.
T142 7624-7754 Sentence denotes GATA-3 was excised from pOTB7 using XhoI digestion and pEGFP-C2 (Clontech, Saint-Germain-en-Laye, France) was digested with BamHI.
T143 7755-7951 Sentence denotes DNA was recovered by phenol extraction and ethanol precipitation, and both the GATA-3 fragment and the pEGFP-C2 vector blunt-ended by incubation with Klenow (Bioline Bio-27029) for 30 min at 37°C.
T144 7952-8008 Sentence denotes Klenow was inactivated by incubation for 10 min at 75°C.
T145 8009-8279 Sentence denotes DNA was recovered by phenol extraction and ethanol precipitation, and both the blunt-ended GATA-3 fragment and the GFP vector were subsequently digested with EcoRI before the 5′-EcoRI/3′–blunt end GATA-3 fragment was inserted into the 5′-EcoRI/3′–blunt ended GFP vector.
T146 8280-8394 Sentence denotes Positive clones were confirmed by digestion and size analysis by 1% agarose gel electrophoresis and by sequencing.
T147 8396-8408 Sentence denotes Transfection
T148 8409-8680 Sentence denotes HuT-78 cells were transfected with either EP8 or GFP vector only DNA using solution R, programme V-001 at a ratio of 3×106 cells/4 µg DNA for 7–8 h in complete medium (10% bovine serum in RPMI1640+15 L-glutamine) according to the general Amaxa protocol for nucleofection.
T149 8681-8844 Sentence denotes The medium was subsequently changed to 1% RPMI for 24 h before transfected cells were added to anti-CD3/CD28 treated wells and live cell videomicroscopy performed.
T150 8846-8867 Sentence denotes Time-Lapse Microscopy
T151 8868-9136 Sentence denotes HuT-78 cells expressing GATA-3-GFP were maintained at 37°C in growth medium in a closed FCS2 perfusion chamber (Bioptechs, Butler, Pennsylvania, USA) combined with an objective heater (Bioptechs) on the stage a Zeiss Axiovert 200 microscope (Thornwood, New York, USA).
T152 9137-9402 Sentence denotes Observations were made by 40×1.0 NA oil-immersion objective lens, and fluorescence and phase contrast images were gathered using a Hamamatsu ORCA-ER charged coupled device camera (Bridgewater, New Jersey, USA) driven by Openlab software (Improvision, Coventry, UK).
T153 9403-9457 Sentence denotes Photographs were taken at 0, 30, 60, 120, and 240 min.
T154 9459-9481 Sentence denotes Densitometric Analysis
T155 9482-9611 Sentence denotes Densitometry of ECL immunoblots was performed using Gelworks ID intermediate software (Ultraviolet Products, Cambridgeshire, UK).
T156 9612-9692 Sentence denotes Briefly, immunoblots were scanned and gates were drawn tightly around each band.
T157 9693-9772 Sentence denotes Background values from each lane were subtracted to normalize each measurement.
T158 9773-9827 Sentence denotes The bands were quantified using the Gelworks software.
T159 9828-9999 Sentence denotes All Western blots were exposed to film for varying lengths of time, and only films generating subsaturating levels of intensity were selected for densitometric evaluation.
T160 10001-10021 Sentence denotes Statistical Analysis
T161 10022-10236 Sentence denotes Data from three or more independent experiments are presented as the mean±standard error of the mean (SEM), except where stated and were compared using GraphPad Prism 4 (GraphPad Software, http://www.graphpad.com).
T162 10237-10460 Sentence denotes Results were analysed using one-way ANOVA with Newman-Keuls post test except for the data from the in vivo inhaled FP study, which was analysed by Friedman's test with subsequent Wilcoxson matched pair signed rank sum test.
T163 10461-10526 Sentence denotes Data from this analysis are presented as a box-and-whiskers plot.
T164 10527-10676 Sentence denotes Friedman's test was used as three matched measures were obtained using placebo, 100 µg, and 500 µg of inhaled FP, which had variable baseline levels.
T165 10677-10834 Sentence denotes We did not assume a Gaussian distribution of the data due to the limited numbers of participants analysed (seven).The null hypothesis was rejected at p<0.05.

ICD10

Id Subject Object Predicate Lexical cue
T3954 83-89 http://purl.bioontology.org/ontology/ICD10/J45.9 denotes asthma
T3955 276-282 http://purl.bioontology.org/ontology/ICD10/J45.9 denotes asthma
T3956 83-89 http://purl.bioontology.org/ontology/ICD10/J45 denotes asthma
T3957 276-282 http://purl.bioontology.org/ontology/ICD10/J45 denotes asthma

events-check-again

Id Subject Object Predicate Lexical cue
T6048 4352-4354 Protein denotes GR
T6049 4355-4361 Protein denotes GATA-3
T6050 4611-4617 Protein denotes GATA-3
T6051 4717-4719 Protein denotes GR
T6052 4988-4994 Protein denotes GATA-3
T6053 5095-5097 Protein denotes GR
T6054 5492-5498 Protein denotes GATA-3
T6055 5600-5602 Protein denotes GR
T6731 6519-6521 Protein denotes GR
T4329 879-882 Protein denotes CD3
T4330 884-888 Protein denotes CD28
T4331 890-892 Protein denotes GR
T4332 1002-1008 Protein denotes GATA-3
T4333 1020-1022 Protein denotes GR
T4334 1196-1201 Protein denotes ATF-2
T4606 2154-2160 Protein denotes GATA-3
T4607 2161-2173 Localization denotes localization
T4842 2641-2645 Protein denotes IL-4
T5188 3306-3312 Protein denotes GATA-3
T5459 4064-4068 Protein denotes IL-5
T6260 5816-5819 Protein denotes p65
T7155 7453-7459 Protein denotes GATA-3
T7156 7460-7463 Protein denotes GFP
T7157 7478-7484 Protein denotes GATA-3
T7158 7596-7602 Protein denotes GATA-3
T7159 7624-7630 Protein denotes GATA-3
T7160 7660-7664 Protein denotes XhoI
T7161 7748-7753 Protein denotes BamHI
T7162 7834-7840 Protein denotes GATA-3
T7163 8100-8106 Protein denotes GATA-3
T7164 8124-8127 Protein denotes GFP
T7165 8167-8172 Protein denotes EcoRI
T7166 8206-8212 Protein denotes GATA-3
T7167 8268-8271 Protein denotes GFP
T7603 8881-8891 Gene_expression denotes expressing
T7604 8892-8902 Protein denotes GATA-3-GFP
R3967 T4606 T4607 themeOf GATA-3,localization
R6532 T7604 T7603 themeOf GATA-3-GFP,expressing

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T4335 873-882 Protein denotes human CD3
T4336 884-888 Protein denotes CD28
T4337 890-892 Protein denotes GR
T4338 898-908 Protein denotes importin-α
T4339 1020-1022 Protein denotes GR
T4839 2398-2403 Protein denotes RNase
T4840 2641-2645 Protein denotes IL-4
T4841 2700-2705 Protein denotes GADPH
T6056 4393-4403 Protein denotes Importin-α
T6057 4443-4453 Protein denotes importin-α
T6058 4611-4617 Protein denotes GATA-3
T6059 4704-4707 Protein denotes CD3
T6060 4708-4712 Protein denotes CD28
T6061 4717-4719 Protein denotes GR
T6062 4935-4964 Protein denotes goat anti-importin-α antibody
T6063 5070-5080 Protein denotes importin-α
T6064 5413-5439 Protein denotes FITC swine anti-rabbit IgG
T6065 5492-5498 Protein denotes GATA-3
T6066 5532-5535 Protein denotes IgG
T6067 5600-5602 Protein denotes GR
T6068 5479-5488 Gene_expression denotes detection
T6069 5587-5596 Gene_expression denotes detection
T6070 5466-5470 Positive_regulation denotes used
T6732 7237-7257 Protein denotes serum immunoglobulin
T6733 7259-7263 Protein denotes Dako
T7141 7581-7585 Protein denotes XhoI
T7142 7596-7602 Protein denotes GATA-3
T7143 7610-7622 Protein denotes pOTB7 vector
T7144 7624-7630 Protein denotes GATA-3
T7145 7648-7664 Protein denotes pOTB7 using XhoI
T7146 7679-7687 Protein denotes pEGFP-C2
T7147 7748-7753 Protein denotes BamHI
T7148 7635-7642 Negative_regulation denotes excised
T7149 7635-7642 Negative_regulation denotes excised
T7150 7834-7849 Protein denotes GATA-3 fragment
T7151 7858-7863 Protein denotes pEGFP
T7152 7864-7866 Protein denotes C2
T7153 8167-8172 Protein denotes EcoRI
T7154 8206-8221 Protein denotes GATA-3 fragment
T8093 10637-10639 Protein denotes FP
T4610 2054-2074 Protein denotes Th2 cytokine release
T4340 1173-1183 Protein denotes MAP kinase
T4341 1188-1201 Protein denotes phospho-ATF-2
T4342 1400-1403 Protein denotes IgG
T4608 1996-2004 Protein denotes anti-CD3
T4609 2005-2009 Protein denotes CD28
T4611 2044-2053 Positive_regulation denotes stimulate
T5189 3077-3094 Protein denotes complete protease
T5190 3104-3113 Negative_regulation denotes inhibitor
T5191 3306-3312 Protein denotes GATA-3
T5192 3316-3326 Protein denotes importin-α
T5193 3525-3536 Protein denotes anti-GATA-3
T5194 3538-3553 Protein denotes anti-importin-α
T5195 3555-3562 Protein denotes anti-GR
T5196 3564-3574 Protein denotes anti-p-p38
T5197 3575-3585 Protein denotes MAP kinase
T5198 3519-3524 Positive_regulation denotes using
T5460 3727-3736 Protein denotes Chromatin
T5461 3737-3756 Localization denotes Immunoprecipitation
T5462 3757-3766 Protein denotes Chromatin
T5463 3918-3934 Protein denotes immunoglobulin G
T5464 4064-4077 Protein denotes IL-5 promoter
T6261 5706-5711 Protein denotes NF-κB
T6262 5712-5722 Positive_regulation denotes Activation
T6263 5723-5728 Protein denotes NF-κB
T6264 5729-5736 Binding denotes binding
T6265 5934-5965 Protein denotes NF-κB consensus oligonucleotide
T6266 6008-6025 Protein denotes anti-p65 antibody
T6267 6074-6097 Protein denotes HRP-conjugated antibody
T6268 6036-6043 Binding denotes binding
T7345 8776-8784 Protein denotes anti-CD3
T7346 8785-8789 Protein denotes CD28
R3968 T4610 T4611 themeOf Th2 cytokine release,stimulate
R4481 T5189 T5190 themeOf complete protease,inhibitor
R4482 T5196 T5198 themeOf anti-p-p38,using
R4723 T5460 T5461 themeOf Chromatin,Immunoprecipitation
R5223 T6064 T6070 causeOf FITC swine anti-rabbit IgG,used
R5224 T6065 T6068 themeOf GATA-3,detection
R5225 T6067 T6069 themeOf GR,detection
R5226 T6068 T6070 themeOf detection,used
R5386 T6261 T6262 themeOf NF-κB,Activation
R5387 T6263 T6264 themeOf NF-κB,binding
R5388 T6267 T6268 themeOf HRP-conjugated antibody,binding
R6129 T7144 T7148 themeOf GATA-3,excised
R6130 T7145 T7149 themeOf pOTB7 using XhoI,excised

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T3970 879-882 Protein denotes CD3
T3971 884-888 Protein denotes CD28
T3972 890-892 Protein denotes GR
T3973 1002-1008 Protein denotes GATA-3
T3974 1020-1022 Protein denotes GR
T3975 1196-1201 Protein denotes ATF-2
T5481 4352-4354 Protein denotes GR
T5482 4355-4361 Protein denotes GATA-3
T5483 4611-4617 Protein denotes GATA-3
T5484 4717-4719 Protein denotes GR
T5485 4988-4994 Protein denotes GATA-3
T5486 5095-5097 Protein denotes GR
T5487 5492-5498 Protein denotes GATA-3
T5488 5600-5602 Protein denotes GR
T7351 8881-8891 Gene_expression denotes expressing
T7352 8892-8902 Protein denotes GATA-3-GFP
T4614 2641-2645 Protein denotes IL-4
T6272 6519-6521 Protein denotes GR
T6761 7453-7459 Protein denotes GATA-3
T6762 7460-7463 Protein denotes GFP
T6763 7478-7484 Protein denotes GATA-3
T6764 7596-7602 Protein denotes GATA-3
T6765 7624-7630 Protein denotes GATA-3
T6766 7660-7664 Protein denotes XhoI
T6767 7748-7753 Protein denotes BamHI
T6768 7834-7840 Protein denotes GATA-3
T6769 8100-8106 Protein denotes GATA-3
T6770 8124-8127 Protein denotes GFP
T6771 8167-8172 Protein denotes EcoRI
T6772 8206-8212 Protein denotes GATA-3
T6773 8268-8271 Protein denotes GFP
T6073 5816-5819 Protein denotes p65
T4347 2154-2160 Protein denotes GATA-3
T4348 2161-2173 Localization denotes localization
T5201 4064-4068 Protein denotes IL-5
T4845 3306-3312 Protein denotes GATA-3
R6293 T7352 T7351 themeOf GATA-3-GFP,expressing
R3730 T4347 T4348 themeOf GATA-3,localization

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T4128 879-882 P09693 denotes CD3
T4129 879-882 P07766 denotes CD3
T4130 879-882 P04234 denotes CD3
T4131 879-882 P20963 denotes CD3
T4132 884-888 P10747 denotes CD28
T4133 890-892 P04150 denotes GR
T4134 1002-1008 P23771 denotes GATA-3
T4135 1020-1022 P04150 denotes GR
T4136 1169-1172 Q16539 denotes p38
T4137 1169-1172 P53778 denotes p38
T4138 1169-1172 Q15759 denotes p38
T4139 1169-1172 O15264 denotes p38
T4140 1196-1201 P15336 denotes ATF-2
T4141 1468-1470 P04150 denotes GR
T5739 4352-4354 P04150 denotes GR
T5740 4355-4361 P23771 denotes GATA-3
T5741 4611-4617 P23771 denotes GATA-3
T5742 4704-4707 P04234 denotes CD3
T5743 4704-4707 P09693 denotes CD3
T5744 4704-4707 P20963 denotes CD3
T5745 4704-4707 P07766 denotes CD3
T5746 4708-4712 P10747 denotes CD28
T5747 4717-4719 P04150 denotes GR
T5748 4988-4994 P23771 denotes GATA-3
T5749 5095-5097 P04150 denotes GR
T5750 5304-5310 P23771 denotes GATA-3
T5751 5326-5328 P04150 denotes GR
T5752 5492-5498 P23771 denotes GATA-3
T5753 5600-5602 P04150 denotes GR
T6927 7453-7459 P23771 denotes GATA-3
T6928 7460-7463 Q9U6Y4 denotes GFP
T6929 7478-7484 P23771 denotes GATA-3
T6930 7596-7602 P23771 denotes GATA-3
T6931 7624-7630 P23771 denotes GATA-3
T6932 7834-7840 P23771 denotes GATA-3
T6933 8100-8106 P23771 denotes GATA-3
T6934 8124-8127 Q9U6Y4 denotes GFP
T6935 8206-8212 P23771 denotes GATA-3
T6936 8268-8271 Q9U6Y4 denotes GFP
T4464 2001-2004 P04234 denotes CD3
T4465 2001-2004 P20963 denotes CD3
T4466 2001-2004 P09693 denotes CD3
T4467 2001-2004 P07766 denotes CD3
T4468 2005-2009 P10747 denotes CD28
T4469 2154-2160 P23771 denotes GATA-3
T4715 2562-2570 Q04864 denotes relative
T4716 2641-2645 P05112 denotes IL-4
T5004 3036-3038 P0A7Z4 denotes pH
T5005 3306-3312 P23771 denotes GATA-3
T5006 3530-3536 P23771 denotes GATA-3
T5007 3560-3562 P04150 denotes GR
T5008 3571-3574 Q16539 denotes p38
T5009 3571-3574 Q15759 denotes p38
T5010 3571-3574 P53778 denotes p38
T5011 3571-3574 O15264 denotes p38
T5012 3594-3599 P15336 denotes ATF-2
T5316 3871-3877 P23771 denotes GATA-3
T5317 4064-4068 P05113 denotes IL-5
T6157 5816-5819 Q04206 denotes p65
T6158 5816-5819 P21579 denotes p65
T6159 6013-6016 Q04206 denotes p65
T6160 6013-6016 P21579 denotes p65
T6484 6519-6521 P04150 denotes GR
T6485 6567-6573 P23771 denotes GATA-3
T7248 8458-8461 Q9U6Y4 denotes GFP
T7249 8781-8784 P20963 denotes CD3
T7250 8781-8784 P09693 denotes CD3
T7251 8781-8784 P07766 denotes CD3
T7252 8781-8784 P04234 denotes CD3
T7253 8785-8789 P10747 denotes CD28
T7469 8892-8898 P23771 denotes GATA-3
T7470 8899-8902 Q9U6Y4 denotes GFP
T7471 9278-9282 Q13501 denotes ORCA
T7472 9283-9285 P03372 denotes ER
T7690 9543-9545 P41134 denotes ID

test2

Id Subject Object Predicate Lexical cue
T3964 879-882 Protein denotes CD3
T3965 884-888 Protein denotes CD28
T3966 890-892 Protein denotes GR
T3967 1002-1008 Protein denotes GATA-3
T3968 1020-1022 Protein denotes GR
T3969 1196-1201 Protein denotes ATF-2
T4345 2154-2160 Protein denotes GATA-3
T4346 2161-2173 Localization denotes localization
T4613 2641-2645 Protein denotes IL-4
T4844 3306-3312 Protein denotes GATA-3
T5200 4064-4068 Protein denotes IL-5
T5473 4352-4354 Protein denotes GR
T5474 4355-4361 Protein denotes GATA-3
T5475 4611-4617 Protein denotes GATA-3
T5476 4717-4719 Protein denotes GR
T5477 4988-4994 Protein denotes GATA-3
T5478 5095-5097 Protein denotes GR
T5479 5492-5498 Protein denotes GATA-3
T5480 5600-5602 Protein denotes GR
T6072 5816-5819 Protein denotes p65
T6270 6519-6521 Protein denotes GR
T6747 7453-7459 Protein denotes GATA-3
T6748 7460-7463 Protein denotes GFP
T6749 7478-7484 Protein denotes GATA-3
T6750 7596-7602 Protein denotes GATA-3
T6751 7624-7630 Protein denotes GATA-3
T6752 7635-7642 Negative_regulation denotes excised
T6753 7660-7664 Protein denotes XhoI
T6754 7748-7753 Protein denotes BamHI
T6755 7834-7840 Protein denotes GATA-3
T6756 8100-8106 Protein denotes GATA-3
T6757 8124-8127 Protein denotes GFP
T6758 8167-8172 Protein denotes EcoRI
T6759 8206-8212 Protein denotes GATA-3
T6760 8268-8271 Protein denotes GFP
T7349 8881-8891 Gene_expression denotes expressing
T7350 8892-8902 Protein denotes GATA-3-GFP
R5811 T6751 T6752 themeOf GATA-3,excised
R5812 T6756 T6758 themeOf GATA-3,EcoRI
R5813 T6757 T6758 themeOf GFP,EcoRI
R5814 T6759 T6758 themeOf GATA-3,EcoRI
R6292 T7350 T7349 themeOf GATA-3-GFP,expressing
R3729 T4345 T4346 themeOf GATA-3,localization