PMC:2669177 / 39153-39657
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/2669177","sourcedb":"PMC","sourceid":"2669177","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2669177","text":"Protein detection by western blot\nWestern analysis was performed as reported previously [24], [35], [49]. Dissected individual retinas were homogenized on ice and solubilized (50 mM Tris, pH 7.8, 100 mM NaCl, 5 mM EDTA, 0.05% SDS, 1 mM PMSF, 1% TX-100, 2.5% (v/v) glycerol) for one hour at 4°C, then processed for SDS-PAGE and subsequent Western blotting using 10–50 µg protein per lane (as detected by Bradford assay; Bio-Rad). Blots were imaged using a Kodak Image Station 4000R with Kodak MI software.","divisions":[{"label":"title","span":{"begin":0,"end":33}}],"tracks":[{"project":"2_test","denotations":[{"id":"19390689-15277471-97510259","span":{"begin":89,"end":91},"obj":"15277471"},{"id":"19390689-15277471-97510259","span":{"begin":89,"end":91},"obj":"15277471"},{"id":"19390689-15254014-97510260","span":{"begin":95,"end":97},"obj":"15254014"},{"id":"19390689-15254014-97510260","span":{"begin":95,"end":97},"obj":"15254014"},{"id":"19390689-12766040-97510261","span":{"begin":101,"end":103},"obj":"12766040"},{"id":"19390689-12766040-97510261","span":{"begin":101,"end":103},"obj":"12766040"},{"id":"19390689-15277471-97510259","span":{"begin":89,"end":91},"obj":"15277471"},{"id":"19390689-15277471-97510259","span":{"begin":89,"end":91},"obj":"15277471"},{"id":"19390689-15254014-97510260","span":{"begin":95,"end":97},"obj":"15254014"},{"id":"19390689-15254014-97510260","span":{"begin":95,"end":97},"obj":"15254014"},{"id":"19390689-12766040-97510261","span":{"begin":101,"end":103},"obj":"12766040"},{"id":"19390689-12766040-97510261","span":{"begin":101,"end":103},"obj":"12766040"}],"attributes":[{"subj":"19390689-15277471-97510259","pred":"source","obj":"2_test"},{"subj":"19390689-15277471-97510259","pred":"source","obj":"2_test"},{"subj":"19390689-15254014-97510260","pred":"source","obj":"2_test"},{"subj":"19390689-15254014-97510260","pred":"source","obj":"2_test"},{"subj":"19390689-12766040-97510261","pred":"source","obj":"2_test"},{"subj":"19390689-12766040-97510261","pred":"source","obj":"2_test"},{"subj":"19390689-15277471-97510259","pred":"source","obj":"2_test"},{"subj":"19390689-15277471-97510259","pred":"source","obj":"2_test"},{"subj":"19390689-15254014-97510260","pred":"source","obj":"2_test"},{"subj":"19390689-15254014-97510260","pred":"source","obj":"2_test"},{"subj":"19390689-12766040-97510261","pred":"source","obj":"2_test"},{"subj":"19390689-12766040-97510261","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ecc093","default":true}]}]}}