PMC:2669177 / 29910-30950 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/2669177","sourcedb":"PMC","sourceid":"2669177","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2669177","text":"Due to the more pronounced functional and structural rescue in eyes treated with IRBP-NMP nanoparticles (compared to CBA-NMP), we conclude that IRBP is the preferred promoter for this application and will take advantage of this finding in future studies directed at therapeutic intervention in hereditary photoreceptor degenerations. It is not clear to us why IRBP-NMP particles lead to more pronounced cone rescue than do CBA-NMP particles in spite of demonstrating similar levels of mRNA expression (Figure 1), but it is possible that the two transcripts are differently regulated. While the IRBP promoter drives gene expression in both rods and cones, qualitative results from transgenic mice made with the same transgene as the IRBP-NMP nanoparticles (Figure S5 and [24]) suggest that IRBP-mediated gene expression is much higher in cones than in rods. The enhanced cone rescue in IRBP-NMP (compared to CBA-NMP) treated eyes is likely a result of this inherent ability of the IRBP promoter to drive higher transgene expression in cones.","tracks":[{"project":"2_test","denotations":[{"id":"19390689-15277471-97510244","span":{"begin":771,"end":773},"obj":"15277471"},{"id":"19390689-15277471-97510244","span":{"begin":771,"end":773},"obj":"15277471"},{"id":"19390689-15277471-97510244","span":{"begin":771,"end":773},"obj":"15277471"},{"id":"19390689-15277471-97510244","span":{"begin":771,"end":773},"obj":"15277471"}],"attributes":[{"subj":"19390689-15277471-97510244","pred":"source","obj":"2_test"},{"subj":"19390689-15277471-97510244","pred":"source","obj":"2_test"},{"subj":"19390689-15277471-97510244","pred":"source","obj":"2_test"},{"subj":"19390689-15277471-97510244","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93adec","default":true}]}]}}