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PMC:2664230 / 6724-8503 JSONTXT

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bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T3248 1493-1500 Positive_regulation denotes induced
T3249 1501-1506 Protein denotes TNF-α
T3250 1507-1517 Gene_expression denotes production
T3251 1670-1683 Positive_regulation denotes up-regulation
T3252 1688-1703 Negative_regulation denotes down-regulation
T3253 1707-1712 Protein denotes TNF-α
T3254 1713-1723 Gene_expression denotes production
R2595 T3249 T3250 themeOf TNF-α,production
R2596 T3250 T3248 themeOf production,induced
R2597 T3253 T3254 themeOf TNF-α,production
R2598 T3254 T3251 themeOf production,up-regulation
R2599 T3254 T3252 themeOf production,down-regulation

2_test

Id Subject Object Predicate Lexical cue
18568240-17689696-22101119 263-265 17689696 denotes 10

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T3577 0-5 JJ denotes Human
T3578 6-16 NNP denotes Peripheral
T3579 17-22 NNP denotes Blood
T3580 23-34 NNP denotes Mononuclear
T3581 35-39 NNP denotes Cell
T3582 40-49 NN denotes Isolation
T3583 50-53 CC denotes and
T3584 54-65 NN denotes Stimulation
T3585 66-71 JJ denotes Human
T3586 72-83 JJ denotes mononuclear
T3587 84-89 NN denotes cells
T3588 90-94 VB denotes were
T3589 95-103 VB denotes isolated
T3590 104-108 IN denotes from
T3591 109-112 DT denotes the
T3592 113-123 JJ denotes peripheral
T3593 124-129 NN denotes blood
T3594 130-132 IN denotes of
T3595 133-137 CD denotes four
T3596 138-145 JJ denotes healthy
T3597 146-151 JJ denotes human
T3598 152-162 NN denotes volunteers
T3599 164-167 DT denotes The
T3600 168-174 NN denotes sample
T3601 175-179 NN denotes size
T3602 180-183 VB denotes was
T3603 184-190 VB denotes chosen
T3604 191-195 IN denotes from
T3605 196-199 PRP-DOLLAR- denotes our
T3606 200-210 RB denotes previously
T3607 211-220 VB denotes published
T3608 221-225 NN denotes work
T3609 225-226 -COMMA- denotes ,
T3610 227-232 WDT denotes which
T3611 233-245 VB denotes demonstrated
T3612 246-254 JJ denotes reliable
T3613 255-265 NN denotes results.10
T3614 266-270 NN denotes Cell
T3615 271-280 NN denotes isolation
T3616 281-284 CC denotes and
T3617 285-288 DT denotes all
T3618 289-299 JJ denotes subsequent
T3619 300-311 NN denotes experiments
T3620 312-316 VB denotes were
T3621 317-326 VB denotes conducted
T3622 327-332 IN denotes under
T3623 333-340 JJ denotes sterile
T3624 341-344 CC denotes and
T3625 345-357 JJ denotes pyrogen-free
T3626 358-368 NN denotes conditions
T3627 370-375 NN denotes Blood
T3628 376-385 VB denotes collected
T3629 386-388 IN denotes in
T3630 389-396 NN denotes heparin
T3631 397-402 NN denotes tubes
T3632 403-406 VB denotes was
T3633 407-416 VB denotes incubated
T3634 417-421 IN denotes with
T3635 422-429 NN denotes Dextran
T3636 430-434 NN denotes T500
T3637 435-438 CC denotes and
T3638 439-442 DT denotes the
T3639 443-446 JJ denotes red
T3640 447-452 NN denotes cells
T3641 453-457 VB denotes were
T3642 458-468 VB denotes sedimented
T3643 469-472 IN denotes for
T3644 473-475 CD denotes 40
T3645 476-483 NN denotes minutes
T3646 484-486 IN denotes at
T3647 487-491 NN denotes room
T3648 492-503 NN denotes temperature
T3649 505-508 DT denotes The
T3650 509-518 JJ denotes resultant
T3651 519-524 NN denotes serum
T3652 525-528 VB denotes was
T3653 529-533 RB denotes then
T3654 534-540 VB denotes washed
T3655 541-545 IN denotes with
T3656 546-550 NN denotes HBSS
T3657 551-554 CC denotes and
T3658 555-564 VB denotes separated
T3659 565-567 IN denotes by
T3660 568-575 NNP denotes Percoll
T3661 576-584 NN denotes gradient
T3662 585-599 NN denotes centrifugation
T3663 600-609 VB denotes according
T3664 610-612 TO denotes to
T3665 613-616 DT denotes the
T3666 617-631 NN denotes manufacturer’s
T3667 632-644 NN denotes instructions
T3668 646-650 NN denotes Cell
T3669 651-660 NN denotes viability
T3670 661-664 VB denotes was
T3671 665-673 VB denotes assessed
T3672 674-676 IN denotes by
T3673 677-683 NN denotes trypan
T3674 684-688 JJ denotes blue
T3675 689-692 NN denotes dye
T3676 693-702 NN denotes exclusion
T3677 702-703 -COMMA- denotes ,
T3678 704-708 IN denotes with
T3679 709-715 NN denotes purity
T3680 716-718 IN denotes of
T3681 719-726 JJ denotes greater
T3682 727-731 IN denotes than
T3683 732-734 CD denotes 95
T3684 734-735 NN denotes %
T3685 737-745 VB denotes Isolated
T3686 746-751 NN denotes cells
T3687 752-756 VB denotes were
T3688 757-768 VB denotes resuspended
T3689 769-771 IN denotes in
T3690 772-776 NN denotes RPMI
T3691 777-781 CD denotes 1640
T3692 782-794 VB denotes supplemented
T3693 795-799 IN denotes with
T3694 800-802 CD denotes 10
T3695 802-803 NN denotes %
T3696 804-807 NN denotes FBS
T3697 808-811 CC denotes and
T3698 812-813 CD denotes 5
T3699 814-816 NN denotes mM
T3700 817-822 NN denotes HEPES
T3701 823-825 IN denotes at
T3702 826-827 DT denotes a
T3703 828-841 NN denotes concentration
T3704 842-844 IN denotes of
T3705 845-846 CD denotes 1
T3706 847-848 SYM denotes x
T3707 849-860 NN denotes 107cells/mL
T3708 862-866 DT denotes Each
T3709 867-877 JJ denotes subsequent
T3710 878-888 NN denotes experiment
T3711 889-895 VB denotes listed
T3712 896-901 IN denotes below
T3713 902-905 VB denotes was
T3714 906-915 VB denotes conducted
T3715 916-918 IN denotes on
T3716 919-926 NN denotes samples
T3717 927-929 IN denotes of
T3718 930-931 CD denotes 5
T3719 932-933 NN denotes x
T3720 934-937 CD denotes 106
T3721 938-943 NN denotes cells
T3722 944-947 IN denotes per
T3723 948-957 NN denotes treatment
T3724 958-963 NN denotes group
T3725 965-973 VB denotes Isolated
T3726 974-979 NN denotes cells
T3727 980-984 VB denotes were
T3728 985-995 VB denotes stimulated
T3729 996-1000 IN denotes with
T3730 1001-1007 CC denotes either
T3731 1008-1012 NN denotes HBSS
T3732 1013-1015 IN denotes as
T3733 1016-1017 DT denotes a
T3734 1018-1026 JJ denotes negative
T3735 1027-1034 NN denotes control
T3736 1034-1035 -COMMA- denotes ,
T3737 1036-1039 NN denotes LPS
T3738 1040-1041 -LRB- denotes (
T3739 1041-1042 CD denotes 1
T3740 1043-1048 NN denotes μg/mL
T3741 1048-1049 -RRB- denotes )
T3742 1049-1050 -COMMA- denotes ,
T3743 1051-1054 NN denotes PTX
T3744 1055-1056 -LRB- denotes (
T3745 1056-1058 CD denotes 20
T3746 1059-1061 NN denotes mM
T3747 1061-1062 -RRB- denotes )
T3748 1062-1063 -COMMA- denotes ,
T3749 1064-1066 CC denotes or
T3750 1067-1078 JJ denotes concomitant
T3751 1079-1082 NN denotes LPS
T3752 1083-1084 SYM denotes /
T3753 1085-1088 NN denotes PTX
T3754 1088-1089 -COMMA- denotes ,
T3755 1090-1092 IN denotes at
T3756 1093-1096 DT denotes the
T3757 1097-1112 JJ denotes above-mentioned
T3758 1113-1127 NN denotes concentrations
T3759 1127-1128 -COMMA- denotes ,
T3760 1129-1132 IN denotes for
T3761 1133-1135 CD denotes 30
T3762 1136-1143 NN denotes minutes
T3763 1144-1146 IN denotes at
T3764 1147-1151 NN denotes 37ºC
T3765 1153-1158 NN denotes Cells
T3766 1159-1163 VB denotes were
T3767 1164-1170 VB denotes placed
T3768 1171-1173 IN denotes on
T3769 1174-1177 NN denotes ice
T3770 1178-1181 IN denotes for
T3771 1182-1184 CD denotes 10
T3772 1185-1192 NN denotes minutes
T3773 1193-1195 TO denotes to
T3774 1196-1200 VB denotes stop
T3775 1201-1204 DT denotes the
T3776 1205-1213 NN denotes reaction
T3777 1214-1217 CC denotes and
T3778 1218-1221 DT denotes the
T3779 1222-1229 NN denotes samples
T3780 1230-1234 VB denotes were
T3781 1235-1239 RB denotes then
T3782 1240-1246 VB denotes stored
T3783 1247-1249 IN denotes at
T3784 1250-1255 NN denotes −70°C
T3785 1256-1259 IN denotes for
T3786 1260-1267 JJ denotes further
T3787 1268-1276 NN denotes analysis
T3788 1278-1281 DT denotes The
T3789 1282-1293 NN denotes stimulation
T3790 1294-1299 NN denotes times
T3791 1300-1303 CC denotes and
T3792 1304-1318 NN denotes concentrations
T3793 1319-1321 IN denotes of
T3794 1322-1325 NN denotes LPS
T3795 1326-1329 CC denotes and
T3796 1330-1333 NN denotes PTX
T3797 1334-1342 VB denotes utilized
T3798 1343-1345 IN denotes in
T3799 1346-1350 DT denotes this
T3800 1351-1356 NN denotes study
T3801 1357-1361 VB denotes were
T3802 1362-1372 VB denotes determined
T3803 1373-1375 IN denotes in
T3804 1376-1384 JJ denotes previous
T3805 1385-1390 NN denotes pilot
T3806 1391-1398 NN denotes studies
T3807 1399-1403 VB denotes done
T3808 1404-1406 IN denotes in
T3809 1407-1410 PRP-DOLLAR- denotes our
T3810 1411-1421 NN denotes laboratory
T3811 1421-1422 -COMMA- denotes ,
T3812 1423-1428 WDT denotes which
T3813 1429-1437 VB denotes examined
T3814 1438-1441 DT denotes the
T3815 1442-1449 NN denotes effects
T3816 1450-1452 IN denotes of
T3817 1453-1463 VB denotes increasing
T3818 1464-1478 NN denotes concentrations
T3819 1479-1481 IN denotes of
T3820 1482-1485 NN denotes PTX
T3821 1486-1488 IN denotes on
T3822 1489-1500 JJ denotes LPS-induced
T3823 1501-1506 NN denotes TNF-α
T3824 1507-1517 NN denotes production
T3825 1519-1522 NN denotes LPS
T3826 1523-1525 IN denotes at
T3827 1526-1527 DT denotes a
T3828 1528-1541 NN denotes concentration
T3829 1542-1544 IN denotes of
T3830 1545-1546 CD denotes 1
T3831 1547-1552 NN denotes μg/mL
T3832 1553-1556 CC denotes and
T3833 1557-1560 NN denotes PTX
T3834 1561-1563 IN denotes at
T3835 1564-1565 DT denotes a
T3836 1566-1579 NN denotes concentration
T3837 1580-1582 IN denotes of
T3838 1583-1585 CD denotes 20
T3839 1586-1588 NN denotes mM
T3840 1589-1593 VB denotes were
T3841 1594-1597 DT denotes the
T3842 1598-1605 JJ denotes minimum
T3843 1606-1620 NN denotes concentrations
T3844 1621-1630 JJ denotes necessary
T3845 1631-1633 TO denotes to
T3846 1634-1641 VB denotes produce
T3847 1642-1643 DT denotes a
T3848 1644-1656 JJ denotes reproducible
T3849 1657-1660 CC denotes and
T3850 1661-1669 JJ denotes reliable
T3851 1670-1683 NN denotes up-regulation
T3852 1684-1687 CC denotes and
T3853 1688-1703 NN denotes down-regulation
T3854 1704-1706 IN denotes in
T3855 1707-1712 NN denotes TNF-α
T3856 1713-1723 NN denotes production
T3857 1723-1724 -COMMA- denotes ,
T3858 1725-1737 RB denotes respectively
T3859 1737-1738 -COMMA- denotes ,
T3860 1739-1741 IN denotes in
T3861 1742-1754 JJ denotes quantitative
T3862 1755-1761 NN denotes assays
T3863 1762-1763 -LRB- denotes (
T3864 1763-1767 NN denotes data
T3865 1768-1771 RB denotes not
T3866 1772-1777 VB denotes shown
T3867 1777-1778 -RRB- denotes )
R2910 T3581 T3578 arg1Of Cell,Peripheral
R2911 T3581 T3579 arg1Of Cell,Blood
R2912 T3581 T3580 arg1Of Cell,Mononuclear
R2913 T3582 T3583 arg1Of Isolation,and
R2914 T3583 T3577 arg1Of and,Human
R2915 T3583 T3581 arg1Of and,Cell
R2916 T3584 T3583 arg2Of Stimulation,and
R2917 T3587 T3585 arg1Of cells,Human
R2918 T3587 T3586 arg1Of cells,mononuclear
R2919 T3587 T3588 arg1Of cells,were
R2920 T3587 T3589 arg2Of cells,isolated
R2921 T3589 T3588 arg2Of isolated,were
R2922 T3589 T3590 arg1Of isolated,from
R2923 T3593 T3590 arg2Of blood,from
R2924 T3593 T3591 arg1Of blood,the
R2925 T3593 T3592 arg1Of blood,peripheral
R2926 T3593 T3594 arg1Of blood,of
R2927 T3598 T3594 arg2Of volunteers,of
R2928 T3598 T3595 arg1Of volunteers,four
R2929 T3598 T3596 arg1Of volunteers,healthy
R2930 T3598 T3597 arg1Of volunteers,human
R2931 T3601 T3599 arg1Of size,The
R2932 T3601 T3600 arg1Of size,sample
R2933 T3601 T3602 arg1Of size,was
R2934 T3601 T3603 arg2Of size,chosen
R2935 T3603 T3602 arg2Of chosen,was
R2936 T3603 T3604 arg1Of chosen,from
R2937 T3603 T3616 arg1Of chosen,and
R2938 T3607 T3606 arg1Of published,previously
R2939 T3608 T3604 arg2Of work,from
R2940 T3608 T3605 arg1Of work,our
R2941 T3608 T3607 arg1Of work,published
R2942 T3608 T3609 arg1Of work,","
R2943 T3608 T3610 arg1Of work,which
R2944 T3608 T3611 arg1Of work,demonstrated
R2945 T3615 T3611 arg2Of isolation,demonstrated
R2946 T3615 T3612 arg1Of isolation,reliable
R2947 T3615 T3613 arg1Of isolation,results.10
R2948 T3615 T3614 arg1Of isolation,Cell
R2949 T3619 T3617 arg1Of experiments,all
R2950 T3619 T3618 arg1Of experiments,subsequent
R2951 T3619 T3620 arg1Of experiments,were
R2952 T3619 T3621 arg2Of experiments,conducted
R2953 T3621 T3616 arg2Of conducted,and
R2954 T3621 T3620 arg2Of conducted,were
R2955 T3621 T3622 arg1Of conducted,under
R2956 T3623 T3624 arg1Of sterile,and
R2957 T3625 T3624 arg2Of pyrogen-free,and
R2958 T3626 T3622 arg2Of conditions,under
R2959 T3626 T3623 arg1Of conditions,sterile
R2960 T3626 T3625 arg1Of conditions,pyrogen-free
R2961 T3627 T3628 arg2Of Blood,collected
R2962 T3627 T3632 arg1Of Blood,was
R2963 T3627 T3633 arg2Of Blood,incubated
R2964 T3628 T3629 arg1Of collected,in
R2965 T3631 T3629 arg2Of tubes,in
R2966 T3631 T3630 arg1Of tubes,heparin
R2967 T3633 T3632 arg2Of incubated,was
R2968 T3633 T3634 arg1Of incubated,with
R2969 T3633 T3637 arg1Of incubated,and
R2970 T3636 T3634 arg2Of T500,with
R2971 T3636 T3635 arg1Of T500,Dextran
R2972 T3640 T3638 arg1Of cells,the
R2973 T3640 T3639 arg1Of cells,red
R2974 T3640 T3641 arg1Of cells,were
R2975 T3640 T3642 arg2Of cells,sedimented
R2976 T3642 T3637 arg2Of sedimented,and
R2977 T3642 T3641 arg2Of sedimented,were
R2978 T3642 T3643 arg1Of sedimented,for
R2979 T3645 T3643 arg2Of minutes,for
R2980 T3645 T3644 arg1Of minutes,40
R2981 T3645 T3646 arg1Of minutes,at
R2982 T3648 T3646 arg2Of temperature,at
R2983 T3648 T3647 arg1Of temperature,room
R2984 T3651 T3649 arg1Of serum,The
R2985 T3651 T3650 arg1Of serum,resultant
R2986 T3651 T3652 arg1Of serum,was
R2987 T3651 T3654 arg2Of serum,washed
R2988 T3651 T3658 arg2Of serum,separated
R2989 T3654 T3655 arg1Of washed,with
R2990 T3654 T3657 arg1Of washed,and
R2991 T3656 T3655 arg2Of HBSS,with
R2992 T3657 T3652 arg2Of and,was
R2993 T3657 T3653 arg1Of and,then
R2994 T3658 T3657 arg2Of separated,and
R2995 T3658 T3663 arg1Of separated,according
R2996 T3662 T3658 arg1Of centrifugation,separated
R2997 T3662 T3659 arg2Of centrifugation,by
R2998 T3662 T3660 arg1Of centrifugation,Percoll
R2999 T3662 T3661 arg1Of centrifugation,gradient
R3000 T3664 T3663 arg2Of to,according
R3001 T3667 T3664 arg2Of instructions,to
R3002 T3667 T3665 arg1Of instructions,the
R3003 T3667 T3666 arg1Of instructions,manufacturer’s
R3004 T3669 T3668 arg1Of viability,Cell
R3005 T3669 T3670 arg1Of viability,was
R3006 T3669 T3671 arg2Of viability,assessed
R3007 T3671 T3670 arg2Of assessed,was
R3008 T3671 T3677 arg1Of assessed,","
R3009 T3671 T3678 arg1Of assessed,with
R3010 T3676 T3671 arg1Of exclusion,assessed
R3011 T3676 T3672 arg2Of exclusion,by
R3012 T3676 T3673 arg1Of exclusion,trypan
R3013 T3676 T3674 arg1Of exclusion,blue
R3014 T3676 T3675 arg1Of exclusion,dye
R3015 T3679 T3678 arg2Of purity,with
R3016 T3679 T3680 arg1Of purity,of
R3017 T3681 T3682 arg1Of greater,than
R3018 T3683 T3682 arg2Of 95,than
R3019 T3684 T3680 arg2Of %,of
R3020 T3684 T3681 arg1Of %,greater
R3021 T3686 T3685 arg2Of cells,Isolated
R3022 T3686 T3687 arg1Of cells,were
R3023 T3686 T3688 arg2Of cells,resuspended
R3024 T3688 T3687 arg2Of resuspended,were
R3025 T3688 T3689 arg1Of resuspended,in
R3026 T3688 T3701 arg1Of resuspended,at
R3027 T3690 T3689 arg2Of RPMI,in
R3028 T3690 T3691 arg1Of RPMI,1640
R3029 T3690 T3692 arg2Of RPMI,supplemented
R3030 T3692 T3693 arg1Of supplemented,with
R3031 T3696 T3694 arg1Of FBS,10
R3032 T3696 T3695 arg1Of FBS,%
R3033 T3696 T3697 arg1Of FBS,and
R3034 T3697 T3693 arg2Of and,with
R3035 T3698 T3699 arg1Of 5,mM
R3036 T3700 T3697 arg2Of HEPES,and
R3037 T3700 T3698 arg1Of HEPES,5
R3038 T3703 T3701 arg2Of concentration,at
R3039 T3703 T3702 arg1Of concentration,a
R3040 T3703 T3704 arg1Of concentration,of
R3041 T3707 T3704 arg2Of 107cells/mL,of
R3042 T3707 T3705 arg1Of 107cells/mL,1
R3043 T3707 T3706 arg1Of 107cells/mL,x
R3044 T3710 T3708 arg1Of experiment,Each
R3045 T3710 T3709 arg1Of experiment,subsequent
R3046 T3710 T3711 arg2Of experiment,listed
R3047 T3710 T3713 arg1Of experiment,was
R3048 T3710 T3714 arg2Of experiment,conducted
R3049 T3711 T3712 arg1Of listed,below
R3050 T3714 T3713 arg2Of conducted,was
R3051 T3714 T3715 arg1Of conducted,on
R3052 T3716 T3715 arg2Of samples,on
R3053 T3716 T3717 arg1Of samples,of
R3054 T3721 T3717 arg2Of cells,of
R3055 T3721 T3718 arg1Of cells,5
R3056 T3721 T3719 arg1Of cells,x
R3057 T3721 T3720 arg1Of cells,106
R3058 T3721 T3722 arg1Of cells,per
R3059 T3724 T3722 arg2Of group,per
R3060 T3724 T3723 arg1Of group,treatment
R3061 T3726 T3725 arg2Of cells,Isolated
R3062 T3726 T3727 arg1Of cells,were
R3063 T3726 T3728 arg2Of cells,stimulated
R3064 T3728 T3727 arg2Of stimulated,were
R3065 T3728 T3729 arg1Of stimulated,with
R3066 T3728 T3732 arg1Of stimulated,as
R3067 T3731 T3729 arg2Of HBSS,with
R3068 T3731 T3730 arg1Of HBSS,either
R3069 T3735 T3732 arg2Of control,as
R3070 T3735 T3733 arg1Of control,a
R3071 T3735 T3734 arg1Of control,negative
R3072 T3735 T3736 arg1Of control,","
R3073 T3737 T3738 arg1Of LPS,(
R3074 T3737 T3742 arg1Of LPS,","
R3075 T3740 T3738 arg2Of μg/mL,(
R3076 T3740 T3739 arg1Of μg/mL,1
R3077 T3741 T3738 arg3Of ),(
R3078 T3742 T3749 arg1Of ",",or
R3079 T3743 T3742 arg2Of PTX,","
R3080 T3743 T3744 arg1Of PTX,(
R3081 T3746 T3744 arg2Of mM,(
R3082 T3746 T3745 arg1Of mM,20
R3083 T3747 T3744 arg3Of ),(
R3084 T3749 T3736 arg2Of or,","
R3085 T3749 T3748 arg1Of or,","
R3086 T3749 T3754 arg1Of or,","
R3087 T3749 T3755 arg1Of or,at
R3088 T3749 T3759 arg1Of or,","
R3089 T3749 T3760 arg1Of or,for
R3090 T3753 T3749 arg2Of PTX,or
R3091 T3753 T3750 arg1Of PTX,concomitant
R3092 T3753 T3751 arg1Of PTX,LPS
R3093 T3753 T3752 arg1Of PTX,/
R3094 T3758 T3755 arg2Of concentrations,at
R3095 T3758 T3756 arg1Of concentrations,the
R3096 T3758 T3757 arg1Of concentrations,above-mentioned
R3097 T3762 T3760 arg2Of minutes,for
R3098 T3762 T3761 arg1Of minutes,30
R3099 T3762 T3763 arg1Of minutes,at
R3100 T3764 T3763 arg2Of 37ºC,at
R3101 T3765 T3766 arg1Of Cells,were
R3102 T3765 T3767 arg2Of Cells,placed
R3103 T3767 T3766 arg2Of placed,were
R3104 T3767 T3768 arg1Of placed,on
R3105 T3767 T3777 arg1Of placed,and
R3106 T3769 T3768 arg2Of ice,on
R3107 T3769 T3770 arg1Of ice,for
R3108 T3772 T3770 arg2Of minutes,for
R3109 T3772 T3771 arg1Of minutes,10
R3110 T3772 T3774 arg1Of minutes,stop
R3111 T3774 T3770 arg3Of stop,for
R3112 T3774 T3773 arg1Of stop,to
R3113 T3776 T3774 arg2Of reaction,stop
R3114 T3776 T3775 arg1Of reaction,the
R3115 T3779 T3778 arg1Of samples,the
R3116 T3779 T3780 arg1Of samples,were
R3117 T3779 T3782 arg2Of samples,stored
R3118 T3782 T3777 arg2Of stored,and
R3119 T3782 T3780 arg2Of stored,were
R3120 T3782 T3781 arg1Of stored,then
R3121 T3782 T3783 arg1Of stored,at
R3122 T3784 T3783 arg2Of −70°C,at
R3123 T3784 T3785 arg1Of −70°C,for
R3124 T3787 T3785 arg2Of analysis,for
R3125 T3787 T3786 arg1Of analysis,further
R3126 T3790 T3789 arg1Of times,stimulation
R3127 T3790 T3791 arg1Of times,and
R3128 T3791 T3788 arg1Of and,The
R3129 T3791 T3801 arg1Of and,were
R3130 T3791 T3802 arg2Of and,determined
R3131 T3792 T3791 arg2Of concentrations,and
R3132 T3792 T3793 arg1Of concentrations,of
R3133 T3794 T3795 arg1Of LPS,and
R3134 T3795 T3793 arg2Of and,of
R3135 T3795 T3797 arg2Of and,utilized
R3136 T3796 T3795 arg2Of PTX,and
R3137 T3797 T3798 arg1Of utilized,in
R3138 T3800 T3798 arg2Of study,in
R3139 T3800 T3799 arg1Of study,this
R3140 T3802 T3801 arg2Of determined,were
R3141 T3802 T3803 arg1Of determined,in
R3142 T3806 T3803 arg2Of studies,in
R3143 T3806 T3804 arg1Of studies,previous
R3144 T3806 T3805 arg1Of studies,pilot
R3145 T3806 T3807 arg2Of studies,done
R3146 T3807 T3808 arg1Of done,in
R3147 T3810 T3808 arg2Of laboratory,in
R3148 T3810 T3809 arg1Of laboratory,our
R3149 T3810 T3811 arg1Of laboratory,","
R3150 T3810 T3812 arg1Of laboratory,which
R3151 T3810 T3813 arg1Of laboratory,examined
R3152 T3815 T3813 arg2Of effects,examined
R3153 T3815 T3814 arg1Of effects,the
R3154 T3815 T3816 arg1Of effects,of
R3155 T3815 T3821 arg1Of effects,on
R3156 T3818 T3816 arg2Of concentrations,of
R3157 T3818 T3817 arg1Of concentrations,increasing
R3158 T3818 T3819 arg1Of concentrations,of
R3159 T3820 T3819 arg2Of PTX,of
R3160 T3824 T3821 arg2Of production,on
R3161 T3824 T3822 arg1Of production,LPS-induced
R3162 T3824 T3823 arg1Of production,TNF-α
R3163 T3825 T3826 arg1Of LPS,at
R3164 T3825 T3834 arg1Of LPS,at
R3165 T3825 T3840 arg1Of LPS,were
R3166 T3828 T3826 arg2Of concentration,at
R3167 T3828 T3827 arg1Of concentration,a
R3168 T3828 T3829 arg1Of concentration,of
R3169 T3831 T3832 arg1Of μg/mL,and
R3170 T3832 T3829 arg2Of and,of
R3171 T3832 T3830 arg1Of and,1
R3172 T3833 T3832 arg2Of PTX,and
R3173 T3836 T3834 arg2Of concentration,at
R3174 T3836 T3835 arg1Of concentration,a
R3175 T3836 T3837 arg1Of concentration,of
R3176 T3839 T3837 arg2Of mM,of
R3177 T3839 T3838 arg1Of mM,20
R3178 T3843 T3840 arg2Of concentrations,were
R3179 T3843 T3841 arg1Of concentrations,the
R3180 T3843 T3842 arg1Of concentrations,minimum
R3181 T3843 T3844 arg1Of concentrations,necessary
R3182 T3846 T3844 arg2Of produce,necessary
R3183 T3846 T3845 arg1Of produce,to
R3184 T3846 T3857 arg1Of produce,","
R3185 T3846 T3858 arg1Of produce,respectively
R3186 T3846 T3859 arg1Of produce,","
R3187 T3846 T3860 arg1Of produce,in
R3188 T3848 T3849 arg1Of reproducible,and
R3189 T3850 T3849 arg2Of reliable,and
R3190 T3851 T3852 arg1Of up-regulation,and
R3191 T3852 T3846 arg2Of and,produce
R3192 T3852 T3847 arg1Of and,a
R3193 T3852 T3848 arg1Of and,reproducible
R3194 T3852 T3850 arg1Of and,reliable
R3195 T3852 T3854 arg1Of and,in
R3196 T3853 T3852 arg2Of down-regulation,and
R3197 T3856 T3854 arg2Of production,in
R3198 T3856 T3855 arg1Of production,TNF-α
R3199 T3862 T3860 arg2Of assays,in
R3200 T3862 T3861 arg1Of assays,quantitative
R3201 T3862 T3863 arg1Of assays,(
R3202 T3864 T3863 arg2Of data,(
R3203 T3864 T3866 arg2Of data,shown
R3204 T3866 T3865 arg1Of shown,not
R3205 T3867 T3863 arg3Of ),(

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T3267 0-5 NNP denotes Human
T3268 6-16 NNP denotes Peripheral
T3269 17-22 NNP denotes Blood
T3270 23-34 NNP denotes Mononuclear
T3271 35-39 NNP denotes Cell
T3272 40-49 NNP denotes Isolation
T3273 50-53 CC denotes and
T3274 54-65 NNP denotes Stimulation
T3275 66-71 NNP denotes Human
T3276 72-83 NN denotes mononuclear
T3277 84-89 NNS denotes cells
T3278 90-94 VBD denotes were
T3279 95-103 VBN denotes isolated
T3280 104-108 IN denotes from
T3281 109-112 DT denotes the
T3282 113-123 JJ denotes peripheral
T3283 124-129 NN denotes blood
T3284 130-132 IN denotes of
T3285 133-137 CD denotes four
T3286 138-145 JJ denotes healthy
T3287 146-151 JJ denotes human
T3288 152-162 NNS denotes volunteers
T3289 162-163 . denotes .
T3290 164-167 DT denotes The
T3291 168-174 NN denotes sample
T3292 175-179 NN denotes size
T3293 180-183 VBD denotes was
T3294 184-190 VBN denotes chosen
T3295 191-195 IN denotes from
T3296 196-199 PRP$ denotes our
T3297 200-210 RB denotes previously
T3298 211-220 VBN denotes published
T3299 221-225 NN denotes work
T3300 225-226 , denotes ,
T3301 227-232 WDT denotes which
T3302 233-245 VBD denotes demonstrated
T3303 246-254 JJ denotes reliable
T3304 255-262 NNS denotes results
T3305 262-265 CD denotes .10
T3306 266-270 NNP denotes Cell
T3307 271-280 NN denotes isolation
T3308 281-284 CC denotes and
T3309 285-288 DT denotes all
T3310 289-299 JJ denotes subsequent
T3311 300-311 NNS denotes experiments
T3312 312-316 VBD denotes were
T3313 317-326 VBN denotes conducted
T3314 327-332 IN denotes under
T3315 333-340 JJ denotes sterile
T3316 341-344 CC denotes and
T3317 345-357 JJ denotes pyrogen-free
T3318 358-368 NNS denotes conditions
T3319 368-369 . denotes .
T3320 370-375 NNP denotes Blood
T3321 376-385 VBN denotes collected
T3322 386-388 IN denotes in
T3323 389-396 NN denotes heparin
T3324 397-402 NNS denotes tubes
T3325 403-406 VBD denotes was
T3326 407-416 VBN denotes incubated
T3327 417-421 IN denotes with
T3328 422-429 NNP denotes Dextran
T3329 430-434 CD denotes T500
T3330 435-438 CC denotes and
T3331 439-442 DT denotes the
T3332 443-446 JJ denotes red
T3333 447-452 NNS denotes cells
T3334 453-457 VBD denotes were
T3335 458-468 VBN denotes sedimented
T3336 469-472 IN denotes for
T3337 473-475 CD denotes 40
T3338 476-483 NNS denotes minutes
T3339 484-486 IN denotes at
T3340 487-491 NN denotes room
T3341 492-503 NN denotes temperature
T3342 503-504 . denotes .
T3343 505-508 DT denotes The
T3344 509-518 JJ denotes resultant
T3345 519-524 NN denotes serum
T3346 525-528 VBD denotes was
T3347 529-533 RB denotes then
T3348 534-540 VBN denotes washed
T3349 541-545 IN denotes with
T3350 546-550 NNP denotes HBSS
T3351 551-554 CC denotes and
T3352 555-564 VBN denotes separated
T3353 565-567 IN denotes by
T3354 568-575 NNP denotes Percoll
T3355 576-584 NN denotes gradient
T3356 585-599 NN denotes centrifugation
T3357 600-609 VBG denotes according
T3358 610-612 TO denotes to
T3359 613-616 DT denotes the
T3360 617-629 NN denotes manufacturer
T3361 632-644 NNS denotes instructions
T3362 644-645 . denotes .
T3363 646-650 NNP denotes Cell
T3364 651-660 NN denotes viability
T3365 661-664 VBD denotes was
T3366 665-673 VBN denotes assessed
T3367 674-676 IN denotes by
T3368 677-683 NN denotes trypan
T3369 684-688 JJ denotes blue
T3370 689-692 NN denotes dye
T3371 693-702 NN denotes exclusion
T3372 702-703 , denotes ,
T3373 704-708 IN denotes with
T3374 709-715 NN denotes purity
T3375 716-718 IN denotes of
T3376 719-726 JJR denotes greater
T3377 727-731 IN denotes than
T3378 732-734 CD denotes 95
T3379 734-735 NN denotes %
T3380 735-736 . denotes .
T3381 737-745 VBN denotes Isolated
T3382 746-751 NNS denotes cells
T3383 752-756 VBD denotes were
T3384 757-768 VBN denotes resuspended
T3385 769-771 IN denotes in
T3386 772-776 NNP denotes RPMI
T3387 777-781 CD denotes 1640
T3388 782-794 VBN denotes supplemented
T3389 795-799 IN denotes with
T3390 800-802 CD denotes 10
T3391 802-803 NN denotes %
T3392 804-807 NNP denotes FBS
T3393 808-811 CC denotes and
T3394 812-813 CD denotes 5
T3395 814-816 NNP denotes mM
T3396 817-822 NNP denotes HEPES
T3397 823-825 IN denotes at
T3398 826-827 DT denotes a
T3399 828-841 NN denotes concentration
T3400 842-844 IN denotes of
T3401 845-846 CD denotes 1
T3402 847-848 LS denotes x
T3403 849-860 NNP denotes 107cells/mL
T3404 860-861 . denotes .
T3405 862-866 DT denotes Each
T3406 867-877 JJ denotes subsequent
T3407 878-888 NN denotes experiment
T3408 889-895 VBN denotes listed
T3409 896-901 RB denotes below
T3410 902-905 VBD denotes was
T3411 906-915 VBN denotes conducted
T3412 916-918 IN denotes on
T3413 919-926 NNS denotes samples
T3414 927-929 IN denotes of
T3415 930-931 CD denotes 5
T3416 932-933 LS denotes x
T3417 934-937 CD denotes 106
T3418 938-943 NNS denotes cells
T3419 944-947 IN denotes per
T3420 948-957 NN denotes treatment
T3421 958-963 NN denotes group
T3422 963-964 . denotes .
T3423 965-973 VBN denotes Isolated
T3424 974-979 NNS denotes cells
T3425 980-984 VBD denotes were
T3426 985-995 VBN denotes stimulated
T3427 996-1000 IN denotes with
T3428 1001-1007 DT denotes either
T3429 1008-1012 NNP denotes HBSS
T3430 1013-1015 IN denotes as
T3431 1016-1017 DT denotes a
T3432 1018-1026 JJ denotes negative
T3433 1027-1034 NN denotes control
T3434 1034-1035 , denotes ,
T3435 1036-1039 NNP denotes LPS
T3436 1040-1041 -LRB- denotes (
T3437 1041-1042 CD denotes 1
T3438 1043-1045 NN denotes μg
T3439 1045-1046 NN denotes /
T3440 1046-1048 NNP denotes mL
T3441 1048-1049 -RRB- denotes )
T3442 1049-1050 , denotes ,
T3443 1051-1054 NNP denotes PTX
T3444 1055-1056 -LRB- denotes (
T3445 1056-1058 CD denotes 20
T3446 1059-1061 NNP denotes mM
T3447 1061-1062 -RRB- denotes )
T3448 1062-1063 , denotes ,
T3449 1064-1066 CC denotes or
T3450 1067-1078 JJ denotes concomitant
T3451 1079-1082 NNP denotes LPS
T3452 1083-1084 NN denotes /
T3453 1085-1088 NNP denotes PTX
T3454 1088-1089 , denotes ,
T3455 1090-1092 IN denotes at
T3456 1093-1096 DT denotes the
T3457 1097-1112 JJ denotes above-mentioned
T3458 1113-1127 NNS denotes concentrations
T3459 1127-1128 , denotes ,
T3460 1129-1132 IN denotes for
T3461 1133-1135 CD denotes 30
T3462 1136-1143 NNS denotes minutes
T3463 1144-1146 IN denotes at
T3464 1147-1151 NNP denotes 37ºC
T3465 1151-1152 . denotes .
T3466 1153-1158 NNS denotes Cells
T3467 1159-1163 VBD denotes were
T3468 1164-1170 VBN denotes placed
T3469 1171-1173 IN denotes on
T3470 1174-1177 NN denotes ice
T3471 1178-1181 IN denotes for
T3472 1182-1184 CD denotes 10
T3473 1185-1192 NNS denotes minutes
T3474 1193-1195 TO denotes to
T3475 1196-1200 VB denotes stop
T3476 1201-1204 DT denotes the
T3477 1205-1213 NN denotes reaction
T3478 1214-1217 CC denotes and
T3479 1218-1221 DT denotes the
T3480 1222-1229 NNS denotes samples
T3481 1230-1234 VBD denotes were
T3482 1235-1239 RB denotes then
T3483 1240-1246 VBN denotes stored
T3484 1247-1249 IN denotes at
T3485 1250-1251 CD denotes −
T3486 1251-1253 CD denotes 70
T3487 1253-1254 NN denotes °
T3488 1254-1255 NNP denotes C
T3489 1256-1259 IN denotes for
T3490 1260-1267 JJ denotes further
T3491 1268-1276 NN denotes analysis
T3492 1276-1277 . denotes .
T3493 1278-1281 DT denotes The
T3494 1282-1293 NN denotes stimulation
T3495 1294-1299 NNS denotes times
T3496 1300-1303 CC denotes and
T3497 1304-1318 NNS denotes concentrations
T3498 1319-1321 IN denotes of
T3499 1322-1325 NNP denotes LPS
T3500 1326-1329 CC denotes and
T3501 1330-1333 NNP denotes PTX
T3502 1334-1342 VBD denotes utilized
T3503 1343-1345 IN denotes in
T3504 1346-1350 DT denotes this
T3505 1351-1356 NN denotes study
T3506 1357-1361 VBD denotes were
T3507 1362-1372 VBN denotes determined
T3508 1373-1375 IN denotes in
T3509 1376-1384 JJ denotes previous
T3510 1385-1390 NN denotes pilot
T3511 1391-1398 NNS denotes studies
T3512 1399-1403 VBN denotes done
T3513 1404-1406 IN denotes in
T3514 1407-1410 PRP$ denotes our
T3515 1411-1421 NN denotes laboratory
T3516 1421-1422 , denotes ,
T3517 1423-1428 WDT denotes which
T3518 1429-1437 VBD denotes examined
T3519 1438-1441 DT denotes the
T3520 1442-1449 NNS denotes effects
T3521 1450-1452 IN denotes of
T3522 1453-1463 VBG denotes increasing
T3523 1464-1478 NNS denotes concentrations
T3524 1479-1481 IN denotes of
T3525 1482-1485 NNP denotes PTX
T3526 1486-1488 IN denotes on
T3527 1489-1500 JJ denotes LPS-induced
T3528 1501-1506 JJ denotes TNF-α
T3529 1507-1517 NN denotes production
T3530 1517-1518 . denotes .
T3531 1519-1522 NNP denotes LPS
T3532 1523-1525 IN denotes at
T3533 1526-1527 DT denotes a
T3534 1528-1541 NN denotes concentration
T3535 1542-1544 IN denotes of
T3536 1545-1546 CD denotes 1
T3537 1547-1549 NN denotes μg
T3538 1549-1550 NN denotes /
T3539 1550-1552 NNP denotes mL
T3540 1553-1556 CC denotes and
T3541 1557-1560 NNP denotes PTX
T3542 1561-1563 IN denotes at
T3543 1564-1565 DT denotes a
T3544 1566-1579 NN denotes concentration
T3545 1580-1582 IN denotes of
T3546 1583-1585 CD denotes 20
T3547 1586-1588 NN denotes mM
T3548 1589-1593 VBD denotes were
T3549 1594-1597 DT denotes the
T3550 1598-1605 JJ denotes minimum
T3551 1606-1620 NNS denotes concentrations
T3552 1621-1630 JJ denotes necessary
T3553 1631-1633 TO denotes to
T3554 1634-1641 VB denotes produce
T3555 1642-1643 DT denotes a
T3556 1644-1656 JJ denotes reproducible
T3557 1657-1660 CC denotes and
T3558 1661-1669 JJ denotes reliable
T3559 1670-1683 NN denotes up-regulation
T3560 1684-1687 CC denotes and
T3561 1688-1703 NN denotes down-regulation
T3562 1704-1706 IN denotes in
T3563 1707-1712 JJ denotes TNF-α
T3564 1713-1723 NN denotes production
T3565 1723-1724 , denotes ,
T3566 1725-1737 RB denotes respectively
T3567 1737-1738 , denotes ,
T3568 1739-1741 IN denotes in
T3569 1742-1754 JJ denotes quantitative
T3570 1755-1761 NNS denotes assays
T3571 1762-1763 -LRB- denotes (
T3572 1763-1767 NNS denotes data
T3573 1768-1771 RB denotes not
T3574 1772-1777 VBN denotes shown
T3575 1777-1778 -RRB- denotes )
T3576 1778-1779 . denotes .
R2600 T3267 T3268 compound Human,Peripheral
R2601 T3268 T3269 compound Peripheral,Blood
R2602 T3269 T3272 compound Blood,Isolation
R2603 T3270 T3272 compound Mononuclear,Isolation
R2604 T3271 T3272 compound Cell,Isolation
R2605 T3272 T3279 nsubjpass Isolation,isolated
R2606 T3273 T3272 cc and,Isolation
R2607 T3274 T3275 compound Stimulation,Human
R2608 T3275 T3277 compound Human,cells
R2609 T3276 T3277 compound mononuclear,cells
R2610 T3277 T3272 conj cells,Isolation
R2611 T3278 T3279 auxpass were,isolated
R2612 T3279 T3279 ROOT isolated,isolated
R2613 T3280 T3279 prep from,isolated
R2614 T3281 T3283 det the,blood
R2615 T3282 T3283 compound peripheral,blood
R2616 T3283 T3280 pobj blood,from
R2617 T3284 T3283 prep of,blood
R2618 T3285 T3288 nummod four,volunteers
R2619 T3286 T3288 amod healthy,volunteers
R2620 T3287 T3288 amod human,volunteers
R2621 T3288 T3284 pobj volunteers,of
R2622 T3289 T3279 punct .,isolated
R2623 T3290 T3292 det The,size
R2624 T3291 T3292 compound sample,size
R2625 T3292 T3294 nsubjpass size,chosen
R2626 T3293 T3294 auxpass was,chosen
R2627 T3294 T3294 ROOT chosen,chosen
R2628 T3295 T3294 prep from,chosen
R2629 T3296 T3299 poss our,work
R2630 T3297 T3298 advmod previously,published
R2631 T3298 T3299 amod published,work
R2632 T3299 T3295 pobj work,from
R2633 T3300 T3299 punct ",",work
R2634 T3301 T3302 nsubj which,demonstrated
R2635 T3302 T3299 relcl demonstrated,work
R2636 T3303 T3304 amod reliable,results
R2637 T3304 T3302 dobj results,demonstrated
R2638 T3305 T3307 nummod .10,isolation
R2639 T3306 T3307 compound Cell,isolation
R2640 T3307 T3313 nsubjpass isolation,conducted
R2641 T3308 T3307 cc and,isolation
R2642 T3309 T3311 det all,experiments
R2643 T3310 T3311 amod subsequent,experiments
R2644 T3311 T3313 nsubjpass experiments,conducted
R2645 T3312 T3313 auxpass were,conducted
R2646 T3313 T3294 advcl conducted,chosen
R2647 T3314 T3313 prep under,conducted
R2648 T3315 T3318 amod sterile,conditions
R2649 T3316 T3315 cc and,sterile
R2650 T3317 T3315 conj pyrogen-free,sterile
R2651 T3318 T3314 pobj conditions,under
R2652 T3319 T3313 punct .,conducted
R2653 T3320 T3321 nsubj Blood,collected
R2654 T3321 T3321 ROOT collected,collected
R2655 T3322 T3321 prep in,collected
R2656 T3323 T3324 compound heparin,tubes
R2657 T3324 T3322 pobj tubes,in
R2658 T3325 T3326 auxpass was,incubated
R2659 T3326 T3321 conj incubated,collected
R2660 T3327 T3335 mark with,sedimented
R2661 T3328 T3335 nsubjpass Dextran,sedimented
R2662 T3329 T3328 nummod T500,Dextran
R2663 T3330 T3328 cc and,Dextran
R2664 T3331 T3333 det the,cells
R2665 T3332 T3333 amod red,cells
R2666 T3333 T3328 conj cells,Dextran
R2667 T3334 T3335 auxpass were,sedimented
R2668 T3335 T3326 advcl sedimented,incubated
R2669 T3336 T3335 prep for,sedimented
R2670 T3337 T3338 nummod 40,minutes
R2671 T3338 T3336 pobj minutes,for
R2672 T3339 T3338 prep at,minutes
R2673 T3340 T3341 compound room,temperature
R2674 T3341 T3339 pobj temperature,at
R2675 T3342 T3321 punct .,collected
R2676 T3343 T3345 det The,serum
R2677 T3344 T3345 amod resultant,serum
R2678 T3345 T3348 nsubjpass serum,washed
R2679 T3346 T3348 auxpass was,washed
R2680 T3347 T3348 advmod then,washed
R2681 T3348 T3348 ROOT washed,washed
R2682 T3349 T3348 prep with,washed
R2683 T3350 T3349 pobj HBSS,with
R2684 T3351 T3348 cc and,washed
R2685 T3352 T3348 conj separated,washed
R2686 T3353 T3352 agent by,separated
R2687 T3354 T3356 nmod Percoll,centrifugation
R2688 T3355 T3356 compound gradient,centrifugation
R2689 T3356 T3353 pobj centrifugation,by
R2690 T3357 T3348 prep according,washed
R2691 T3358 T3357 prep to,according
R2692 T3359 T3360 det the,manufacturer
R2693 T3360 T3361 poss manufacturer,instructions
R2694 T3361 T3358 pobj instructions,to
R2695 T3362 T3348 punct .,washed
R2696 T3363 T3364 compound Cell,viability
R2697 T3364 T3366 nsubjpass viability,assessed
R2698 T3365 T3366 auxpass was,assessed
R2699 T3366 T3366 ROOT assessed,assessed
R2700 T3367 T3366 agent by,assessed
R2701 T3368 T3371 compound trypan,exclusion
R2702 T3369 T3370 amod blue,dye
R2703 T3370 T3371 compound dye,exclusion
R2704 T3371 T3367 pobj exclusion,by
R2705 T3372 T3371 punct ",",exclusion
R2706 T3373 T3366 prep with,assessed
R2707 T3374 T3373 pobj purity,with
R2708 T3375 T3374 prep of,purity
R2709 T3376 T3378 amod greater,95
R2710 T3377 T3378 quantmod than,95
R2711 T3378 T3379 nummod 95,%
R2712 T3379 T3375 pobj %,of
R2713 T3380 T3366 punct .,assessed
R2714 T3381 T3382 amod Isolated,cells
R2715 T3382 T3384 nsubjpass cells,resuspended
R2716 T3383 T3384 auxpass were,resuspended
R2717 T3384 T3384 ROOT resuspended,resuspended
R2718 T3385 T3384 prep in,resuspended
R2719 T3386 T3388 nsubj RPMI,supplemented
R2720 T3387 T3386 nummod 1640,RPMI
R2721 T3388 T3384 conj supplemented,resuspended
R2722 T3389 T3388 prep with,supplemented
R2723 T3390 T3391 nummod 10,%
R2724 T3391 T3392 compound %,FBS
R2725 T3392 T3389 pobj FBS,with
R2726 T3393 T3392 cc and,FBS
R2727 T3394 T3396 nummod 5,HEPES
R2728 T3395 T3396 compound mM,HEPES
R2729 T3396 T3392 conj HEPES,FBS
R2730 T3397 T3388 prep at,supplemented
R2731 T3398 T3399 det a,concentration
R2732 T3399 T3397 pobj concentration,at
R2733 T3400 T3399 prep of,concentration
R2734 T3401 T3403 nummod 1,107cells/mL
R2735 T3402 T3403 compound x,107cells/mL
R2736 T3403 T3400 pobj 107cells/mL,of
R2737 T3404 T3388 punct .,supplemented
R2738 T3405 T3407 det Each,experiment
R2739 T3406 T3407 amod subsequent,experiment
R2740 T3407 T3408 nsubj experiment,listed
R2741 T3408 T3408 ROOT listed,listed
R2742 T3409 T3408 advmod below,listed
R2743 T3410 T3411 auxpass was,conducted
R2744 T3411 T3408 dep conducted,listed
R2745 T3412 T3411 prep on,conducted
R2746 T3413 T3412 pobj samples,on
R2747 T3414 T3413 prep of,samples
R2748 T3415 T3418 nummod 5,cells
R2749 T3416 T3418 nmod x,cells
R2750 T3417 T3418 nummod 106,cells
R2751 T3418 T3414 pobj cells,of
R2752 T3419 T3418 prep per,cells
R2753 T3420 T3421 compound treatment,group
R2754 T3421 T3419 pobj group,per
R2755 T3422 T3408 punct .,listed
R2756 T3423 T3424 amod Isolated,cells
R2757 T3424 T3426 nsubjpass cells,stimulated
R2758 T3425 T3426 auxpass were,stimulated
R2759 T3426 T3426 ROOT stimulated,stimulated
R2760 T3427 T3426 prep with,stimulated
R2761 T3428 T3429 det either,HBSS
R2762 T3429 T3427 pobj HBSS,with
R2763 T3430 T3429 prep as,HBSS
R2764 T3431 T3433 det a,control
R2765 T3432 T3433 amod negative,control
R2766 T3433 T3430 pobj control,as
R2767 T3434 T3433 punct ",",control
R2768 T3435 T3433 appos LPS,control
R2769 T3436 T3440 punct (,mL
R2770 T3437 T3438 nummod 1,μg
R2771 T3438 T3440 compound μg,mL
R2772 T3439 T3440 compound /,mL
R2773 T3440 T3435 appos mL,LPS
R2774 T3441 T3440 punct ),mL
R2775 T3442 T3435 punct ",",LPS
R2776 T3443 T3454 csubj PTX,","
R2777 T3444 T3446 punct (,mM
R2778 T3445 T3446 nummod 20,mM
R2779 T3446 T3443 parataxis mM,PTX
R2780 T3447 T3446 punct ),mM
R2781 T3448 T3446 punct ",",mM
R2782 T3449 T3443 cc or,PTX
R2783 T3450 T3452 amod concomitant,/
R2784 T3451 T3452 compound LPS,/
R2785 T3452 T3453 compound /,PTX
R2786 T3453 T3443 conj PTX,PTX
R2787 T3454 T3435 punct ",",LPS
R2788 T3455 T3433 prep at,control
R2789 T3456 T3458 det the,concentrations
R2790 T3457 T3458 amod above-mentioned,concentrations
R2791 T3458 T3455 pobj concentrations,at
R2792 T3459 T3426 punct ",",stimulated
R2793 T3460 T3426 prep for,stimulated
R2794 T3461 T3462 nummod 30,minutes
R2795 T3462 T3460 pobj minutes,for
R2796 T3463 T3462 prep at,minutes
R2797 T3464 T3463 pobj 37ºC,at
R2798 T3465 T3426 punct .,stimulated
R2799 T3466 T3468 nsubjpass Cells,placed
R2800 T3467 T3468 auxpass were,placed
R2801 T3468 T3468 ROOT placed,placed
R2802 T3469 T3468 prep on,placed
R2803 T3470 T3469 pobj ice,on
R2804 T3471 T3468 prep for,placed
R2805 T3472 T3473 nummod 10,minutes
R2806 T3473 T3471 pobj minutes,for
R2807 T3474 T3475 aux to,stop
R2808 T3475 T3468 advcl stop,placed
R2809 T3476 T3477 det the,reaction
R2810 T3477 T3475 dobj reaction,stop
R2811 T3478 T3477 cc and,reaction
R2812 T3479 T3480 det the,samples
R2813 T3480 T3483 nsubjpass samples,stored
R2814 T3481 T3483 auxpass were,stored
R2815 T3482 T3483 advmod then,stored
R2816 T3483 T3468 conj stored,placed
R2817 T3484 T3483 prep at,stored
R2818 T3485 T3488 nummod −,C
R2819 T3486 T3487 nummod 70,°
R2820 T3487 T3488 compound °,C
R2821 T3488 T3484 pobj C,at
R2822 T3489 T3483 prep for,stored
R2823 T3490 T3491 amod further,analysis
R2824 T3491 T3489 pobj analysis,for
R2825 T3492 T3483 punct .,stored
R2826 T3493 T3495 det The,times
R2827 T3494 T3495 compound stimulation,times
R2828 T3495 T3502 nsubj times,utilized
R2829 T3496 T3495 cc and,times
R2830 T3497 T3495 conj concentrations,times
R2831 T3498 T3497 prep of,concentrations
R2832 T3499 T3498 pobj LPS,of
R2833 T3500 T3499 cc and,LPS
R2834 T3501 T3499 conj PTX,LPS
R2835 T3502 T3502 ROOT utilized,utilized
R2836 T3503 T3502 prep in,utilized
R2837 T3504 T3505 det this,study
R2838 T3505 T3503 pobj study,in
R2839 T3506 T3507 auxpass were,determined
R2840 T3507 T3502 conj determined,utilized
R2841 T3508 T3507 prep in,determined
R2842 T3509 T3511 amod previous,studies
R2843 T3510 T3511 compound pilot,studies
R2844 T3511 T3508 pobj studies,in
R2845 T3512 T3511 acl done,studies
R2846 T3513 T3512 prep in,done
R2847 T3514 T3515 poss our,laboratory
R2848 T3515 T3513 pobj laboratory,in
R2849 T3516 T3515 punct ",",laboratory
R2850 T3517 T3518 nsubj which,examined
R2851 T3518 T3511 relcl examined,studies
R2852 T3519 T3520 det the,effects
R2853 T3520 T3518 dobj effects,examined
R2854 T3521 T3520 prep of,effects
R2855 T3522 T3521 pcomp increasing,of
R2856 T3523 T3522 dobj concentrations,increasing
R2857 T3524 T3523 prep of,concentrations
R2858 T3525 T3524 pobj PTX,of
R2859 T3526 T3523 prep on,concentrations
R2860 T3527 T3529 amod LPS-induced,production
R2861 T3528 T3529 compound TNF-α,production
R2862 T3529 T3526 pobj production,on
R2863 T3530 T3502 punct .,utilized
R2864 T3531 T3548 nsubj LPS,were
R2865 T3532 T3531 prep at,LPS
R2866 T3533 T3534 det a,concentration
R2867 T3534 T3532 pobj concentration,at
R2868 T3535 T3534 prep of,concentration
R2869 T3536 T3537 nummod 1,μg
R2870 T3537 T3539 compound μg,mL
R2871 T3538 T3539 compound /,mL
R2872 T3539 T3535 pobj mL,of
R2873 T3540 T3539 cc and,mL
R2874 T3541 T3539 conj PTX,mL
R2875 T3542 T3534 prep at,concentration
R2876 T3543 T3544 det a,concentration
R2877 T3544 T3542 pobj concentration,at
R2878 T3545 T3544 prep of,concentration
R2879 T3546 T3547 nummod 20,mM
R2880 T3547 T3545 pobj mM,of
R2881 T3548 T3548 ROOT were,were
R2882 T3549 T3551 det the,concentrations
R2883 T3550 T3551 amod minimum,concentrations
R2884 T3551 T3548 attr concentrations,were
R2885 T3552 T3551 amod necessary,concentrations
R2886 T3553 T3554 aux to,produce
R2887 T3554 T3552 xcomp produce,necessary
R2888 T3555 T3559 det a,up-regulation
R2889 T3556 T3559 amod reproducible,up-regulation
R2890 T3557 T3556 cc and,reproducible
R2891 T3558 T3556 conj reliable,reproducible
R2892 T3559 T3554 dobj up-regulation,produce
R2893 T3560 T3559 cc and,up-regulation
R2894 T3561 T3559 conj down-regulation,up-regulation
R2895 T3562 T3559 prep in,up-regulation
R2896 T3563 T3564 compound TNF-α,production
R2897 T3564 T3562 pobj production,in
R2898 T3565 T3559 punct ",",up-regulation
R2899 T3566 T3554 advmod respectively,produce
R2900 T3567 T3554 punct ",",produce
R2901 T3568 T3554 prep in,produce
R2902 T3569 T3570 amod quantitative,assays
R2903 T3570 T3568 pobj assays,in
R2904 T3571 T3574 punct (,shown
R2905 T3572 T3574 nsubjpass data,shown
R2906 T3573 T3574 neg not,shown
R2907 T3574 T3548 parataxis shown,were
R2908 T3575 T3574 punct ),shown
R2909 T3576 T3548 punct .,were

UBERON-AE

Id Subject Object Predicate Lexical cue
T3244 17-22 http://purl.obolibrary.org/obo/UBERON_0000178 denotes Blood
T3245 124-129 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T3246 397-402 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tubes
T3247 519-524 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T3884 1501-1517 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T3885 1693-1723 http://purl.obolibrary.org/obo/GO_0032680 denotes regulation in TNF-α production
T3886 1501-1517 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T3887 1707-1723 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T3888 1673-1683 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T3889 1693-1703 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T3890 1693-1712 http://purl.obolibrary.org/obo/GO_0042534 denotes regulation in TNF-α
T3891 1693-1712 http://purl.obolibrary.org/obo/GO_1904467 denotes regulation in TNF-α
T3892 1693-1723 http://purl.obolibrary.org/obo/GO_0032720 denotes regulation in TNF-α production
T3893 1693-1723 http://purl.obolibrary.org/obo/GO_0032760 denotes regulation in TNF-α production

GO-CC

Id Subject Object Predicate Lexical cue
T3901 35-39 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T3902 266-270 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T3903 646-650 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T3904 746-751 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3905 938-943 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3906 974-979 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T3255 0-65 Sentence denotes Human Peripheral Blood Mononuclear Cell Isolation and Stimulation
T3256 66-163 Sentence denotes Human mononuclear cells were isolated from the peripheral blood of four healthy human volunteers.
T3257 164-369 Sentence denotes The sample size was chosen from our previously published work, which demonstrated reliable results.10 Cell isolation and all subsequent experiments were conducted under sterile and pyrogen-free conditions.
T3258 370-504 Sentence denotes Blood collected in heparin tubes was incubated with Dextran T500 and the red cells were sedimented for 40 minutes at room temperature.
T3259 505-645 Sentence denotes The resultant serum was then washed with HBSS and separated by Percoll gradient centrifugation according to the manufacturer’s instructions.
T3260 646-736 Sentence denotes Cell viability was assessed by trypan blue dye exclusion, with purity of greater than 95%.
T3261 737-861 Sentence denotes Isolated cells were resuspended in RPMI 1640 supplemented with 10% FBS and 5 mM HEPES at a concentration of 1 x 107cells/mL.
T3262 862-964 Sentence denotes Each subsequent experiment listed below was conducted on samples of 5 x 106 cells per treatment group.
T3263 965-1152 Sentence denotes Isolated cells were stimulated with either HBSS as a negative control, LPS (1 μg/mL), PTX (20 mM), or concomitant LPS / PTX, at the above-mentioned concentrations, for 30 minutes at 37ºC.
T3264 1153-1277 Sentence denotes Cells were placed on ice for 10 minutes to stop the reaction and the samples were then stored at −70°C for further analysis.
T3265 1278-1518 Sentence denotes The stimulation times and concentrations of LPS and PTX utilized in this study were determined in previous pilot studies done in our laboratory, which examined the effects of increasing concentrations of PTX on LPS-induced TNF-α production.
T3266 1519-1779 Sentence denotes LPS at a concentration of 1 μg/mL and PTX at a concentration of 20 mM were the minimum concentrations necessary to produce a reproducible and reliable up-regulation and down-regulation in TNF-α production, respectively, in quantitative assays (data not shown).
T52 0-65 Sentence denotes Human Peripheral Blood Mononuclear Cell Isolation and Stimulation
T53 66-163 Sentence denotes Human mononuclear cells were isolated from the peripheral blood of four healthy human volunteers.
T54 164-369 Sentence denotes The sample size was chosen from our previously published work, which demonstrated reliable results.10 Cell isolation and all subsequent experiments were conducted under sterile and pyrogen-free conditions.
T55 370-504 Sentence denotes Blood collected in heparin tubes was incubated with Dextran T500 and the red cells were sedimented for 40 minutes at room temperature.
T56 505-645 Sentence denotes The resultant serum was then washed with HBSS and separated by Percoll gradient centrifugation according to the manufacturer’s instructions.
T57 646-736 Sentence denotes Cell viability was assessed by trypan blue dye exclusion, with purity of greater than 95%.
T58 737-861 Sentence denotes Isolated cells were resuspended in RPMI 1640 supplemented with 10% FBS and 5 mM HEPES at a concentration of 1 x 107cells/mL.
T59 862-964 Sentence denotes Each subsequent experiment listed below was conducted on samples of 5 x 106 cells per treatment group.
T60 965-1152 Sentence denotes Isolated cells were stimulated with either HBSS as a negative control, LPS (1 μg/mL), PTX (20 mM), or concomitant LPS / PTX, at the above-mentioned concentrations, for 30 minutes at 37ºC.
T61 1153-1277 Sentence denotes Cells were placed on ice for 10 minutes to stop the reaction and the samples were then stored at −70°C for further analysis.
T62 1278-1518 Sentence denotes The stimulation times and concentrations of LPS and PTX utilized in this study were determined in previous pilot studies done in our laboratory, which examined the effects of increasing concentrations of PTX on LPS-induced TNF-α production.
T63 1519-1779 Sentence denotes LPS at a concentration of 1 μg/mL and PTX at a concentration of 20 mM were the minimum concentrations necessary to produce a reproducible and reliable up-regulation and down-regulation in TNF-α production, respectively, in quantitative assays (data not shown).

events-check-again

Id Subject Object Predicate Lexical cue
T3941 1493-1500 Positive_regulation denotes induced
T3942 1501-1506 Protein denotes TNF-α
T3943 1507-1517 Gene_expression denotes production
T3944 1670-1683 Positive_regulation denotes up-regulation
T3945 1688-1703 Negative_regulation denotes down-regulation
T3946 1707-1712 Protein denotes TNF-α
T3947 1713-1723 Gene_expression denotes production
R3234 T3942 T3943 themeOf TNF-α,production
R3235 T3943 T3941 themeOf production,induced
R3236 T3946 T3947 themeOf TNF-α,production
R3237 T3947 T3944 themeOf production,up-regulation
R3238 T3947 T3945 themeOf production,down-regulation

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T3907 772-776 Protein denotes RPMI
T3908 1085-1088 Protein denotes PTX
T3909 1482-1485 Protein denotes PTX
T3910 1501-1506 Protein denotes TNF-α
T3911 1507-1517 Gene_expression denotes production
T3912 1442-1449 Regulation denotes effects
T3913 1557-1560 Protein denotes PTX
T3914 1707-1712 Protein denotes TNF-α
T3915 1713-1723 Gene_expression denotes production
T3916 1670-1683 Positive_regulation denotes up-regulation
T3917 1688-1703 Negative_regulation denotes down-regulation
T3918 1688-1703 Negative_regulation denotes down-regulation
T3919 1670-1683 Positive_regulation denotes up-regulation
T3920 1670-1683 Positive_regulation denotes up-regulation
R3211 T3909 T3912 causeOf PTX,effects
R3212 T3910 T3911 themeOf TNF-α,production
R3213 T3911 T3912 themeOf production,effects
R3214 T3914 T3915 themeOf TNF-α,production
R3215 T3915 T3916 themeOf production,up-regulation
R3216 T3915 T3918 themeOf production,down-regulation
R3217 T3916 T3917 themeOf up-regulation,down-regulation
R3218 T3917 T3919 themeOf down-regulation,up-regulation
R3219 T3918 T3920 themeOf down-regulation,up-regulation

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T3868 1501-1504 P01375 denotes TNF
T3869 1501-1506 P01375 denotes TNF-α
T3870 1707-1710 P01375 denotes TNF
T3871 1707-1712 P01375 denotes TNF-α

test2

Id Subject Object Predicate Lexical cue
T3236 1442-1449 Regulation denotes effects
T3237 1493-1500 Positive_regulation denotes induced
T3238 1501-1506 Protein denotes TNF-α
T3239 1507-1517 Gene_expression denotes production
T3240 1670-1683 Positive_regulation denotes up-regulation
T3241 1688-1703 Negative_regulation denotes down-regulation
T3242 1707-1712 Protein denotes TNF-α
T3243 1713-1723 Gene_expression denotes production
R2590 T3238 T3239 themeOf TNF-α,production
R2591 T3239 T3237 themeOf production,induced
R2592 T3242 T3243 themeOf TNF-α,production
R2593 T3243 T3241 themeOf production,down-regulation
R2594 T3243 T3240 themeOf production,up-regulation