PMC:2664230 / 172-5223 JSON TXT

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bionlp-st-ge-2016-reference

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T64	411-417	Regulation	denotes	alters
T65	418-423	Protein	denotes	TNF-α
T66	424-434	Gene_expression	denotes	production
T67	435-453	Positive_regulation	denotes	in the presence of
T68	667-681	Phosphorylation	denotes	phosphorylated
T69	694-699	Protein	denotes	I-κBα
T70	719-722	Protein	denotes	p65
T71	888-893	Protein	denotes	TNF-α
T72	1108-1114	Negative_regulation	denotes	reduce
T73	1108-1114	Negative_regulation	denotes	reduce
T74	1127-1132	Protein	denotes	I-κBα
T75	1133-1148	Phosphorylation	denotes	phosphorylation
T76	1158-1161	Protein	denotes	p65
T77	1162-1177	Phosphorylation	denotes	phosphorylation
T78	1352-1360	Localization	denotes	secreted
T79	1379-1384	Protein	denotes	TNF-α
T80	1392-1400	Regulation	denotes	presence
T81	1405-1412	Regulation	denotes	absence	true
T1133	2575-2585	Negative_regulation	denotes	attenuated
T1134	2780-2784	Positive_regulation	denotes	lead
T1135	2792-2806	Negative_regulation	denotes	downregulation
T1136	2810-2815	Protein	denotes	TNF-α
T1137	2816-2826	Gene_expression	denotes	production
T1138	3854-3861	Negative_regulation	denotes	inhibit
T1139	3868-3877	Regulation	denotes	dependent
T1140	3900-3913	Transcription	denotes	transcription
T1141	3935-3940	Protein	denotes	TNF-α
T1142	4024-4038	Negative_regulation	denotes	down-regulates
T1143	4039-4044	Protein	denotes	TNF-α
T1144	4045-4055	Gene_expression	denotes	production
T1145	4703-4707	Regulation	denotes	role		true
T1146	4722-4729	Positive_regulation	denotes	induced
T1147	4730-4735	Protein	denotes	TNF-α
T1148	4736-4746	Gene_expression	denotes	production
T57	32-38	Regulation	denotes	effect		true
T58	122-149	Protein	denotes	tumor necrosis factor-alpha
T59	151-156	Protein	denotes	TNF-α
T60	158-168	Gene_expression	denotes	production
T61	251-261	Gene_expression	denotes	production
T62	265-270	Protein	denotes	TNF-α
T63	271-280	Positive_regulation	denotes	following
T1126	2386-2395	Regulation	denotes	regulator
T1127	2399-2422	Protein	denotes	tumor necrosis factor-α
T1128	2424-2429	Protein	denotes	TNF-α
T1129	2431-2441	Gene_expression	denotes	production
T1130	2444-2449	Protein	denotes	TNF-α
T1131	2552-2557	Protein	denotes	TNF-α
T1132	2558-2567	Gene_expression	denotes	synthesis
R33	T64	T67	themeOf	alters,in the presence of
R34	T65	T66	themeOf	TNF-α,production
R35	T66	T64	themeOf	production,alters
R36	T69	T68	themeOf	I-κBα,phosphorylated
R37	T74	T75	themeOf	I-κBα,phosphorylation
R38	T75	T73	themeOf	phosphorylation,reduce
R39	T76	T77	themeOf	p65,phosphorylation
R40	T77	T72	themeOf	phosphorylation,reduce
R41	T78	T80	themeOf	secreted,presence
R42	T78	T81	themeOf	secreted,absence
R43	T79	T78	themeOf	TNF-α,secreted
R750	T58	T60	themeOf	tumor necrosis factor-alpha,production
R751	T60	T57	themeOf	production,effect
R752	T61	T63	themeOf	production,following
R753	T62	T61	themeOf	TNF-α,production
R832	T1127	T1129	themeOf	tumor necrosis factor-α,production
R833	T1128	T1127	equivalentTo	TNF-α,tumor necrosis factor-α
R834	T1129	T1126	themeOf	production,regulator
R835	T1131	T1132	themeOf	TNF-α,synthesis
R836	T1132	T1133	themeOf	synthesis,attenuated
R837	T1135	T1134	themeOf	downregulation,lead
R838	T1136	T1137	themeOf	TNF-α,production
R839	T1137	T1135	themeOf	production,downregulation
R840	T1139	T1138	themeOf	dependent,inhibit
R841	T1140	T1139	themeOf	transcription,dependent
R842	T1141	T1140	themeOf	TNF-α,transcription
R843	T1143	T1144	themeOf	TNF-α,production
R844	T1144	T1142	themeOf	production,down-regulates
R845	T1146	T1145	themeOf	induced,role
R846	T1147	T1148	themeOf	TNF-α,production
R847	T1148	T1146	themeOf	production,induced

2_test

Id	Subject	Object	Predicate	Lexical cue
18568240-12168567-22101112	2313-2314	12168567	denotes	2
18568240-9442376-22101113	2442-2443	9442376	denotes	3
18568240-2460096-22101114	2708-2709	2460096	denotes	4
18568240-1847694-22101115	3102-3103	1847694	denotes	5
18568240-12829247-22101116	3321-3322	12829247	denotes	6
18568240-12829247-22101117	3637-3638	12829247	denotes	6
18568240-15044179-22101117	3637-3638	15044179	denotes	6
18568240-15221855-22101117	3637-3638	15221855	denotes	6
18568240-8702838-22101118	3946-3947	8702838	denotes	9

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T473	0-4	DT	denotes	this
T474	5-10	NN	denotes	study
T475	11-14	VB	denotes	was
T476	15-17	TO	denotes	to
T477	18-27	VB	denotes	determine
T478	28-31	DT	denotes	the
T479	32-38	NN	denotes	effect
T480	39-41	IN	denotes	of
T481	42-53	JJ	denotes	nonspecific
T482	54-71	NN	denotes	phosphodiesterase
T483	72-82	NN	denotes	inhibition
T484	83-85	IN	denotes	on
T485	86-99	NN	denotes	transcription
T486	100-106	NN	denotes	factor
T487	107-117	NN	denotes	activation
T488	118-121	CC	denotes	and
T489	122-127	NN	denotes	tumor
T490	128-136	NN	denotes	necrosis
T491	137-149	NN	denotes	factor-alpha
T492	150-151	-LRB-	denotes	(
T493	151-156	NN	denotes	TNF-α
T494	156-157	-RRB-	denotes	)
T495	158-168	NN	denotes	production
T496	169-171	IN	denotes	in
T497	172-190	NN	denotes	lipopolysaccharide
T498	191-192	-LRB-	denotes	(
T499	192-195	NN	denotes	LPS
T500	195-196	-RRB-	denotes	)
T501	196-207	JJ	denotes	-stimulated
T502	208-213	JJ	denotes	human
T503	214-225	JJ	denotes	mononuclear
T504	226-231	NN	denotes	cells
T505	234-246	NN	denotes	INTRODUCTION
T506	247-250	DT	denotes	The
T507	251-261	NN	denotes	production
T508	262-264	IN	denotes	of
T509	265-270	NN	denotes	TNF-α
T510	271-280	VB	denotes	following
T511	281-284	NN	denotes	LPS
T512	285-296	NN	denotes	stimulation
T513	297-299	VB	denotes	is
T514	300-303	CD	denotes	one
T515	304-306	IN	denotes	of
T516	307-310	DT	denotes	the
T517	311-314	JJ	denotes	key
T518	315-320	NN	denotes	steps
T519	321-323	IN	denotes	in
T520	324-333	JJ	denotes	bacterial
T521	334-340	NN	denotes	sepsis
T522	341-344	CC	denotes	and
T523	345-357	NN	denotes	inflammation
T524	359-362	DT	denotes	The
T525	363-372	NN	denotes	mechanism
T526	373-375	IN	denotes	by
T527	376-381	WDT	denotes	which
T528	382-399	NN	denotes	phosphodiesterase
T529	400-410	NN	denotes	inhibition
T530	411-417	VB	denotes	alters
T531	418-423	NN	denotes	TNF-α
T532	424-434	NN	denotes	production
T533	435-437	IN	denotes	in
T534	438-441	DT	denotes	the
T535	442-450	NN	denotes	presence
T536	451-453	IN	denotes	of
T537	454-457	NN	denotes	LPS
T538	458-465	VB	denotes	remains
T539	466-473	JJ	denotes	unclear
T540	476-483	NN	denotes	METHODS
T541	484-489	JJ	denotes	Human
T542	490-501	JJ	denotes	mononuclear
T543	502-507	NN	denotes	cells
T544	508-512	VB	denotes	were
T545	513-523	VB	denotes	stimulated
T546	524-528	IN	denotes	with
T547	529-532	NN	denotes	LPS
T548	533-534	-LRB-	denotes	(
T549	534-535	CD	denotes	1
T550	536-541	NN	denotes	μg/mL
T551	541-542	-RRB-	denotes	)
T552	542-543	-COMMA-	denotes	,
T553	544-546	IN	denotes	in
T554	547-550	DT	denotes	the
T555	551-559	NN	denotes	presence
T556	560-563	CC	denotes	and
T557	564-571	NN	denotes	absence
T558	572-574	IN	denotes	of
T559	575-589	NN	denotes	Pentoxifylline
T560	590-591	-LRB-	denotes	(
T561	591-594	NN	denotes	PTX
T562	594-595	-COLON-	denotes	;
T563	596-598	CD	denotes	20
T564	599-601	NN	denotes	mM
T565	601-602	-RRB-	denotes	)
T566	602-603	-COMMA-	denotes	,
T567	604-605	DT	denotes	a
T568	606-617	JJ	denotes	nonspecific
T569	618-635	NN	denotes	phosphodiesterase
T570	636-645	NN	denotes	inhibitor
T571	647-654	JJ	denotes	Western
T572	655-663	NN	denotes	blotting
T573	664-666	IN	denotes	of
T574	667-681	VB	denotes	phosphorylated
T575	682-693	JJ	denotes	cytoplasmic
T576	694-699	NN	denotes	I-κBα
T577	699-700	-COMMA-	denotes	,
T578	701-708	JJ	denotes	nuclear
T579	709-718	NN	denotes	factor-κB
T580	719-722	NN	denotes	p65
T581	723-724	-LRB-	denotes	(
T582	724-729	NN	denotes	NF-κB
T583	729-730	-RRB-	denotes	)
T584	730-731	-COMMA-	denotes	,
T585	732-735	CC	denotes	and
T586	736-743	JJ	denotes	nuclear
T587	744-757	JJ	denotes	cAMP-response
T588	758-765	NN	denotes	element
T589	766-773	NN	denotes	binding
T590	774-781	NN	denotes	protein
T591	782-783	-LRB-	denotes	(
T592	783-787	NN	denotes	CREB
T593	787-788	-RRB-	denotes	)
T594	789-792	VB	denotes	was
T595	793-802	VB	denotes	performed
T596	804-807	NN	denotes	DNA
T597	808-815	NN	denotes	binding
T598	816-818	IN	denotes	of
T599	819-824	NN	denotes	NF-κB
T600	825-828	CC	denotes	and
T601	829-833	NN	denotes	CREB
T602	834-837	VB	denotes	was
T603	838-846	VB	denotes	verified
T604	847-849	IN	denotes	by
T605	850-865	JJ	denotes	electrophoretic
T606	866-874	NN	denotes	mobility
T607	875-880	NN	denotes	shift
T608	881-886	NN	denotes	assay
T609	888-893	NN	denotes	TNF-α
T610	894-900	NN	denotes	levels
T611	901-905	VB	denotes	were
T612	906-916	VB	denotes	determined
T613	917-919	IN	denotes	in
T614	920-923	DT	denotes	the
T615	924-935	NN	denotes	supernatant
T616	936-938	IN	denotes	of
T617	939-949	VB	denotes	stimulated
T618	950-955	NN	denotes	cells
T619	956-958	IN	denotes	in
T620	959-962	DT	denotes	the
T621	963-971	NN	denotes	presence
T622	972-975	CC	denotes	and
T623	976-983	NN	denotes	absence
T624	984-991	NN	denotes	Protein
T625	992-998	NN	denotes	kinase
T626	999-1000	NN	denotes	A
T627	1001-1011	NN	denotes	inhibition
T628	1012-1014	IN	denotes	by
T629	1015-1017	DT	denotes	an
T630	1018-1031	JJ	denotes	enzyme-linked
T631	1032-1045	JJ	denotes	immunosorbent
T632	1046-1051	NN	denotes	assay
T633	1052-1053	-LRB-	denotes	(
T634	1053-1058	NN	denotes	ELISA
T635	1058-1059	-RRB-	denotes	)
T636	1062-1069	NN	denotes	RESULTS
T637	1070-1073	NN	denotes	PTX
T638	1074-1077	VB	denotes	was
T639	1078-1090	VB	denotes	demonstrated
T640	1091-1093	TO	denotes	to
T641	1094-1107	RB	denotes	significantly
T642	1108-1114	VB	denotes	reduce
T643	1115-1126	JJ	denotes	cytoplasmic
T644	1127-1132	NN	denotes	I-κBα
T645	1133-1148	NN	denotes	phosphorylation
T646	1148-1149	-COMMA-	denotes	,
T647	1150-1157	JJ	denotes	nuclear
T648	1158-1161	NN	denotes	p65
T649	1162-1177	NN	denotes	phosphorylation
T650	1177-1178	-COMMA-	denotes	,
T651	1179-1182	CC	denotes	and
T652	1183-1186	DT	denotes	the
T653	1187-1190	NN	denotes	DNA
T654	1191-1198	NN	denotes	binding
T655	1199-1207	NN	denotes	activity
T656	1208-1210	IN	denotes	of
T657	1211-1216	NN	denotes	NF-κB
T658	1218-1220	IN	denotes	In
T659	1221-1229	NN	denotes	contrast
T660	1229-1230	-COMMA-	denotes	,
T661	1231-1234	NN	denotes	PTX
T662	1235-1243	RB	denotes	markedly
T663	1244-1252	VB	denotes	enhanced
T664	1253-1256	DT	denotes	the
T665	1257-1272	NN	denotes	phosphorylation
T666	1273-1276	CC	denotes	and
T667	1277-1280	NN	denotes	DNA
T668	1281-1288	NN	denotes	binding
T669	1289-1297	NN	denotes	activity
T670	1298-1300	IN	denotes	of
T671	1301-1305	NN	denotes	CREB
T672	1307-1312	NN	denotes	Cells
T673	1313-1326	RB	denotes	concomitantly
T674	1327-1334	VB	denotes	treated
T675	1335-1339	IN	denotes	with
T676	1340-1343	NN	denotes	PTX
T677	1344-1347	CC	denotes	and
T678	1348-1351	NN	denotes	LPS
T679	1352-1360	VB	denotes	secreted
T680	1361-1368	JJ	denotes	similar
T681	1369-1375	NN	denotes	levels
T682	1376-1378	IN	denotes	of
T683	1379-1384	NN	denotes	TNF-α
T684	1385-1387	IN	denotes	in
T685	1388-1391	DT	denotes	the
T686	1392-1400	NN	denotes	presence
T687	1401-1404	CC	denotes	and
T688	1405-1412	NN	denotes	absence
T689	1413-1420	NN	denotes	Protein
T690	1421-1427	NN	denotes	kinase
T691	1428-1429	NN	denotes	A
T692	1430-1440	NN	denotes	inhibition
T693	1443-1453	NN	denotes	DISCUSSION
T694	1454-1457	DT	denotes	The
T695	1458-1467	VB	denotes	increased
T696	1468-1473	NN	denotes	level
T697	1474-1476	IN	denotes	of
T698	1477-1481	NN	denotes	cAMP
T699	1482-1486	WDT	denotes	that
T700	1487-1494	VB	denotes	results
T701	1495-1499	IN	denotes	from
T702	1500-1517	NN	denotes	phosphodiesterase
T703	1518-1528	NN	denotes	inhibition
T704	1529-1536	VB	denotes	affects
T705	1537-1548	JJ	denotes	cytoplasmic
T706	1549-1552	CC	denotes	and
T707	1553-1560	JJ	denotes	nuclear
T708	1561-1567	NN	denotes	events
T709	1567-1568	-COMMA-	denotes	,
T710	1569-1578	VB	denotes	resulting
T711	1579-1581	IN	denotes	in
T712	1582-1585	DT	denotes	the
T713	1586-1597	NN	denotes	attenuation
T714	1598-1600	IN	denotes	of
T715	1601-1606	NN	denotes	NF-κB
T716	1607-1610	CC	denotes	and
T717	1611-1614	DT	denotes	the
T718	1615-1625	NN	denotes	activation
T719	1626-1628	IN	denotes	of
T720	1629-1633	NN	denotes	CREB
T721	1634-1649	JJ	denotes	transcriptional
T722	1650-1653	NN	denotes	DNA
T723	1654-1661	NN	denotes	binding
T724	1662-1669	IN	denotes	through
T725	1670-1678	NN	denotes	pathways
T726	1679-1683	WDT	denotes	that
T727	1684-1687	VB	denotes	are
T728	1688-1697	RB	denotes	partially
T729	1698-1705	NN	denotes	Protein
T730	1706-1712	NN	denotes	kinase
T731	1713-1726	JJ	denotes	A-independent
T732	1729-1739	NN	denotes	CONCLUSION
T733	1740-1752	JJ	denotes	PTX-mediated
T734	1753-1770	NN	denotes	phosphodiesterase
T735	1771-1781	NN	denotes	inhibition
T736	1782-1788	VB	denotes	occurs
T737	1789-1798	RB	denotes	partially
T738	1799-1806	IN	denotes	through
T739	1807-1808	DT	denotes	a
T740	1809-1816	NN	denotes	Protein
T741	1817-1823	NN	denotes	kinase
T742	1824-1837	JJ	denotes	A-independent
T743	1838-1845	NN	denotes	pathway
T744	1846-1849	CC	denotes	and
T745	1850-1853	MD	denotes	may
T746	1854-1859	VB	denotes	serve
T747	1860-1862	IN	denotes	as
T748	1863-1864	DT	denotes	a
T749	1865-1871	JJ	denotes	useful
T750	1872-1876	NN	denotes	tool
T751	1877-1879	IN	denotes	in
T752	1880-1883	DT	denotes	the
T753	1884-1895	NN	denotes	attenuation
T754	1896-1898	IN	denotes	of
T755	1899-1910	JJ	denotes	LPS-induced
T756	1911-1923	NN	denotes	inflammation
T1612	1940-1943	DT	denotes	The
T1613	1944-1953	JJ	denotes	bacterial
T1614	1954-1962	NN	denotes	membrane
T1615	1963-1972	NN	denotes	component
T1616	1973-1991	NN	denotes	lipopolysaccharide
T1617	1992-1993	-LRB-	denotes	(
T1618	1993-1996	NN	denotes	LPS
T1619	1996-1997	-RRB-	denotes	)
T1620	1998-2000	VB	denotes	is
T1621	2001-2008	JJ	denotes	capable
T1622	2009-2011	IN	denotes	of
T1623	2012-2022	VB	denotes	initiating
T1624	2023-2038	NN	denotes	phosphorylation
T1625	2039-2042	CC	denotes	and
T1626	2043-2053	NN	denotes	activation
T1627	2054-2056	IN	denotes	of
T1628	2057-2065	JJ	denotes	multiple
T1629	2066-2070	NN	denotes	host
T1630	2071-2084	JJ	denotes	intracellular
T1631	2085-2092	NN	denotes	protein
T1632	2093-2100	NN	denotes	kinases
T1633	2101-2104	CC	denotes	and
T1634	2105-2118	NN	denotes	transcription
T1635	2119-2126	NN	denotes	factors
T1636	2128-2141	NN	denotes	Transcription
T1637	2142-2148	NN	denotes	factor
T1638	2149-2159	NN	denotes	activation
T1639	2159-2160	-COMMA-	denotes	,
T1640	2161-2166	WDT	denotes	which
T1641	2167-2174	VB	denotes	results
T1642	2175-2177	IN	denotes	in
T1643	2178-2181	DT	denotes	the
T1644	2182-2192	NN	denotes	modulation
T1645	2193-2195	IN	denotes	of
T1646	2196-2200	NN	denotes	gene
T1647	2201-2214	NN	denotes	transcription
T1648	2215-2218	CC	denotes	and
T1649	2219-2226	NN	denotes	protein
T1650	2227-2236	NN	denotes	synthesis
T1651	2236-2237	-COMMA-	denotes	,
T1652	2238-2240	VB	denotes	is
T1653	2241-2242	DT	denotes	a
T1654	2243-2251	JJ	denotes	critical
T1655	2252-2259	NN	denotes	element
T1656	2260-2262	IN	denotes	in
T1657	2263-2266	DT	denotes	the
T1658	2267-2274	NN	denotes	defense
T1659	2275-2284	NN	denotes	mechanism
T1660	2285-2287	IN	denotes	of
T1661	2288-2291	DT	denotes	the
T1662	2292-2296	NN	denotes	host
T1663	2297-2303	JJ	denotes	immune
T1664	2304-2314	NN	denotes	system.1,2
T1665	2315-2326	JJ	denotes	LPS-induced
T1666	2327-2340	NN	denotes	transcription
T1667	2341-2347	NN	denotes	factor
T1668	2348-2358	NN	denotes	activation
T1669	2359-2362	VB	denotes	has
T1670	2363-2367	VB	denotes	been
T1671	2368-2373	VB	denotes	shown
T1672	2374-2376	TO	denotes	to
T1673	2377-2379	VB	denotes	be
T1674	2380-2381	DT	denotes	a
T1675	2382-2385	JJ	denotes	key
T1676	2386-2395	NN	denotes	regulator
T1677	2396-2398	IN	denotes	of
T1678	2399-2404	NN	denotes	tumor
T1679	2405-2413	NN	denotes	necrosis
T1680	2414-2422	NN	denotes	factor-α
T1681	2423-2424	-LRB-	denotes	(
T1682	2424-2429	NN	denotes	TNF-α
T1683	2429-2430	-RRB-	denotes	)
T1684	2431-2443	NN	denotes	production.3
T1685	2444-2449	NN	denotes	TNF-α
T1686	2450-2452	VB	denotes	is
T1687	2453-2454	DT	denotes	a
T1688	2455-2461	JJ	denotes	potent
T1689	2462-2478	JJ	denotes	pro-inflammatory
T1690	2479-2487	NN	denotes	cytokine
T1691	2488-2496	VB	denotes	involved
T1692	2497-2499	IN	denotes	in
T1693	2500-2501	DT	denotes	a
T1694	2502-2506	JJ	denotes	wide
T1695	2507-2515	NN	denotes	spectrum
T1696	2516-2518	IN	denotes	of
T1697	2519-2527	JJ	denotes	cellular
T1698	2528-2537	NN	denotes	responses
T1699	2539-2550	RB	denotes	Furthermore
T1700	2550-2551	-COMMA-	denotes	,
T1701	2552-2557	NN	denotes	TNF-α
T1702	2558-2567	NN	denotes	synthesis
T1703	2568-2571	MD	denotes	can
T1704	2572-2574	VB	denotes	be
T1705	2575-2585	VB	denotes	attenuated
T1706	2586-2588	IN	denotes	in
T1707	2589-2595	JJ	denotes	immune
T1708	2596-2601	NN	denotes	cells
T1709	2602-2609	VB	denotes	exposed
T1710	2610-2612	TO	denotes	to
T1711	2613-2630	NN	denotes	phosphodiesterase
T1712	2631-2632	-LRB-	denotes	(
T1713	2632-2635	NN	denotes	PDE
T1714	2635-2636	-RRB-	denotes	)
T1715	2637-2647	NN	denotes	inhibition
T1716	2648-2653	IN	denotes	after
T1717	2654-2663	NN	denotes	challenge
T1718	2664-2666	IN	denotes	by
T1719	2667-2668	DT	denotes	a
T1720	2669-2676	NN	denotes	variety
T1721	2677-2679	IN	denotes	of
T1722	2680-2696	JJ	denotes	pro-inflammatory
T1723	2697-2709	NN	denotes	stimulants.4
T1724	2710-2713	DT	denotes	The
T1725	2714-2723	NN	denotes	signaling
T1726	2724-2734	NN	denotes	mechanisms
T1727	2735-2743	VB	denotes	affected
T1728	2744-2746	IN	denotes	by
T1729	2747-2750	NN	denotes	PDE
T1730	2751-2761	NN	denotes	inhibition
T1731	2761-2762	-COMMA-	denotes	,
T1732	2763-2768	WDT	denotes	which
T1733	2769-2779	RB	denotes	ultimately
T1734	2780-2784	VB	denotes	lead
T1735	2785-2787	TO	denotes	to
T1736	2788-2791	DT	denotes	the
T1737	2792-2806	NN	denotes	downregulation
T1738	2807-2809	IN	denotes	of
T1739	2810-2815	NN	denotes	TNF-α
T1740	2816-2826	NN	denotes	production
T1741	2826-2827	-COMMA-	denotes	,
T1742	2828-2832	VB	denotes	have
T1743	2833-2836	RB	denotes	not
T1744	2837-2841	VB	denotes	been
T1745	2842-2846	RB	denotes	well
T1746	2847-2860	VB	denotes	characterized
T1747	2861-2863	IN	denotes	in
T1748	2864-2876	JJ	denotes	inflammatory
T1749	2877-2882	NN	denotes	cells
T1750	2884-2895	RB	denotes	Classically
T1751	2895-2896	-COMMA-	denotes	,
T1752	2897-2899	PRP	denotes	it
T1753	2900-2903	VB	denotes	has
T1754	2904-2908	VB	denotes	been
T1755	2909-2921	VB	denotes	demonstrated
T1756	2922-2926	IN	denotes	that
T1757	2927-2930	NN	denotes	PDE
T1758	2931-2941	NN	denotes	inhibition
T1759	2942-2949	VB	denotes	results
T1760	2950-2952	IN	denotes	in
T1761	2953-2956	DT	denotes	the
T1762	2957-2970	JJ	denotes	intracellular
T1763	2971-2983	NN	denotes	accumulation
T1764	2984-2986	IN	denotes	of
T1765	2987-2990	DT	denotes	the
T1766	2991-2997	JJ	denotes	second
T1767	2998-3007	NN	denotes	messenger
T1768	3008-3014	JJ	denotes	cyclic
T1769	3015-3042	NN	denotes	adenosine-3,5-monophosphate
T1770	3043-3044	-LRB-	denotes	(
T1771	3044-3048	NN	denotes	cAMP
T1772	3048-3049	-RRB-	denotes	)
T1773	3050-3053	CC	denotes	and
T1774	3054-3064	JJ	denotes	subsequent
T1775	3065-3075	NN	denotes	activation
T1776	3076-3078	IN	denotes	of
T1777	3079-3086	NN	denotes	Protein
T1778	3087-3093	NN	denotes	kinase
T1779	3094-3095	NN	denotes	A
T1780	3096-3097	-LRB-	denotes	(
T1781	3097-3100	NN	denotes	PKA
T1782	3100-3101	-RRB-	denotes	)
T1783	3101-3103	CD	denotes	.5
T1784	3104-3107	NN	denotes	PKA
T1785	3108-3118	NN	denotes	activation
T1786	3119-3123	RB	denotes	then
T1787	3124-3129	VB	denotes	leads
T1788	3130-3132	TO	denotes	to
T1789	3133-3136	DT	denotes	the
T1790	3137-3152	NN	denotes	phosphorylation
T1791	3153-3155	IN	denotes	of
T1792	3156-3159	DT	denotes	the
T1793	3160-3173	NN	denotes	transcription
T1794	3174-3180	NN	denotes	factor
T1795	3181-3194	JJ	denotes	cAMP-response
T1796	3195-3202	NN	denotes	element
T1797	3203-3210	NN	denotes	binding
T1798	3211-3218	NN	denotes	protein
T1799	3219-3220	-LRB-	denotes	(
T1800	3220-3224	NN	denotes	CREB
T1801	3224-3225	-RRB-	denotes	)
T1802	3225-3226	-COMMA-	denotes	,
T1803	3227-3239	NN	denotes	transmission
T1804	3240-3242	IN	denotes	of
T1805	3243-3250	NN	denotes	signals
T1806	3251-3255	IN	denotes	into
T1807	3256-3259	DT	denotes	the
T1808	3260-3267	NN	denotes	nucleus
T1809	3267-3268	-COMMA-	denotes	,
T1810	3269-3272	CC	denotes	and
T1811	3273-3276	DT	denotes	the
T1812	3277-3287	JJ	denotes	subsequent
T1813	3288-3298	NN	denotes	modulation
T1814	3299-3301	IN	denotes	of
T1815	3302-3306	NN	denotes	gene
T1816	3307-3322	NN	denotes	transcription.6
T1817	3323-3327	DT	denotes	This
T1818	3328-3338	RB	denotes	apparently
T1819	3339-3345	JJ	denotes	simple
T1820	3346-3352	JJ	denotes	linear
T1821	3353-3360	NN	denotes	cascade
T1822	3361-3365	VB	denotes	does
T1823	3366-3369	RB	denotes	not
T1824	3370-3375	RB	denotes	fully
T1825	3376-3383	VB	denotes	explain
T1826	3384-3387	DT	denotes	the
T1827	3388-3397	NN	denotes	mechanism
T1828	3398-3400	IN	denotes	by
T1829	3401-3406	WDT	denotes	which
T1830	3407-3409	DT	denotes	an
T1831	3410-3419	NN	denotes	elevation
T1832	3420-3422	IN	denotes	in
T1833	3423-3426	DT	denotes	the
T1834	3427-3440	JJ	denotes	intracellular
T1835	3441-3445	NN	denotes	cAMP
T1836	3446-3451	NN	denotes	level
T1837	3452-3458	VB	denotes	exerts
T1838	3459-3471	JJ	denotes	wide-ranging
T1839	3472-3479	NN	denotes	effects
T1840	3480-3482	IN	denotes	on
T1841	3483-3491	JJ	denotes	multiple
T1842	3492-3500	JJ	denotes	cellular
T1843	3501-3510	NN	denotes	functions
T1844	3512-3517	EX	denotes	There
T1845	3518-3520	VB	denotes	is
T1846	3521-3522	DT	denotes	a
T1847	3523-3530	VB	denotes	growing
T1848	3531-3535	NN	denotes	body
T1849	3536-3538	IN	denotes	of
T1850	3539-3547	NN	denotes	evidence
T1851	3548-3558	VB	denotes	suggesting
T1852	3559-3563	IN	denotes	that
T1853	3564-3568	NN	denotes	cAMP
T1854	3569-3572	MD	denotes	may
T1855	3573-3581	VB	denotes	function
T1856	3582-3589	IN	denotes	through
T1857	3590-3594	CC	denotes	both
T1858	3595-3608	JJ	denotes	PKA-dependent
T1859	3609-3612	CC	denotes	and
T1860	3613-3625	JJ	denotes	-independent
T1861	3626-3640	NN	denotes	mechanisms.6–8
T1862	3641-3652	JJ	denotes	LPS-induced
T1863	3653-3663	NN	denotes	activation
T1864	3664-3666	IN	denotes	of
T1865	3667-3670	DT	denotes	the
T1866	3671-3684	NN	denotes	transcription
T1867	3685-3691	NN	denotes	factor
T1868	3692-3699	JJ	denotes	nuclear
T1869	3700-3709	NN	denotes	factor-κB
T1870	3710-3711	-LRB-	denotes	(
T1871	3711-3716	NN	denotes	NF-κB
T1872	3716-3717	-RRB-	denotes	)
T1873	3718-3721	VB	denotes	has
T1874	3722-3726	RB	denotes	also
T1875	3727-3731	VB	denotes	been
T1876	3732-3735	DT	denotes	the
T1877	3736-3741	NN	denotes	focus
T1878	3742-3744	IN	denotes	of
T1879	3745-3746	DT	denotes	a
T1880	3747-3752	JJ	denotes	great
T1881	3753-3757	NN	denotes	deal
T1882	3758-3760	IN	denotes	of
T1883	3761-3769	NN	denotes	research
T1884	3771-3773	PRP	denotes	It
T1885	3774-3777	VB	denotes	has
T1886	3778-3782	VB	denotes	been
T1887	3783-3790	RB	denotes	clearly
T1888	3791-3803	VB	denotes	demonstrated
T1889	3804-3808	IN	denotes	that
T1890	3809-3815	NN	denotes	agents
T1891	3816-3820	WDT	denotes	that
T1892	3821-3829	VB	denotes	increase
T1893	3830-3843	JJ	denotes	intracellular
T1894	3844-3848	NN	denotes	cAMP
T1895	3849-3853	RB	denotes	also
T1896	3854-3861	VB	denotes	inhibit
T1897	3862-3877	JJ	denotes	NF-κB-dependent
T1898	3878-3894	JJ	denotes	pro-inflammatory
T1899	3895-3899	NN	denotes	gene
T1900	3900-3913	NN	denotes	transcription
T1901	3913-3914	-COMMA-	denotes	,
T1902	3915-3927	RB	denotes	particularly
T1903	3928-3930	IN	denotes	of
T1904	3931-3934	DT	denotes	the
T1905	3935-3940	NN	denotes	TNF-α
T1906	3941-3947	NN	denotes	gene.9
T1907	3948-3959	NNP	denotes	Controversy
T1908	3960-3966	VB	denotes	exists
T1909	3967-3976	VB	denotes	regarding
T1910	3977-3980	DT	denotes	the
T1911	3981-3986	JJ	denotes	exact
T1912	3987-3996	NN	denotes	mechanism
T1913	3996-3997	-LRB-	denotes	(
T1914	3997-3998	NN	denotes	s
T1915	3998-3999	-RRB-	denotes	)
T1916	4000-4002	IN	denotes	by
T1917	4003-4008	WDT	denotes	which
T1918	4009-4012	NN	denotes	PDE
T1919	4013-4023	NN	denotes	inhibition
T1920	4024-4038	VB	denotes	down-regulates
T1921	4039-4044	NN	denotes	TNF-α
T1922	4045-4055	NN	denotes	production
T1923	4057-4070	NN	denotes	Possibilities
T1924	4071-4078	VB	denotes	include
T1925	4078-4079	-COMMA-	denotes	,
T1926	4080-4083	CC	denotes	but
T1927	4084-4087	VB	denotes	are
T1928	4088-4091	RB	denotes	not
T1929	4092-4099	VB	denotes	limited
T1930	4100-4102	TO	denotes	to
T1931	4102-4103	-COMMA-	denotes	,
T1932	4104-4114	NN	denotes	inhibition
T1933	4115-4117	IN	denotes	of
T1934	4118-4123	NN	denotes	NF-κB
T1935	4124-4127	NN	denotes	DNA
T1936	4128-4135	NN	denotes	binding
T1937	4136-4144	NN	denotes	activity
T1938	4144-4145	-COMMA-	denotes	,
T1939	4146-4160	NN	denotes	downregulation
T1940	4161-4163	IN	denotes	of
T1941	4164-4169	NN	denotes	NF-κB
T1942	4170-4185	JJ	denotes	transcriptional
T1943	4186-4194	NN	denotes	activity
T1944	4194-4195	-COMMA-	denotes	,
T1945	4196-4205	VB	denotes	increased
T1946	4206-4210	NN	denotes	CREB
T1947	4211-4221	NN	denotes	activation
T1948	4221-4222	-COMMA-	denotes	,
T1949	4223-4226	CC	denotes	and
T1950	4227-4238	NN	denotes	competition
T1951	4239-4246	IN	denotes	between
T1952	4247-4252	NN	denotes	NF-κB
T1953	4253-4256	CC	denotes	and
T1954	4257-4261	NN	denotes	CREB
T1955	4262-4265	IN	denotes	for
T1956	4266-4272	JJ	denotes	common
T1957	4273-4286	NN	denotes	co-activators
T1958	4287-4291	JJ	denotes	such
T1959	4292-4294	IN	denotes	as
T1960	4295-4299	NN	denotes	CREB
T1961	4300-4307	NN	denotes	binding
T1962	4308-4315	NN	denotes	protein
T1963	4316-4317	-LRB-	denotes	(
T1964	4317-4320	NN	denotes	CBP
T1965	4320-4321	-RRB-	denotes	)
T1966	4323-4325	PRP	denotes	It
T1967	4326-4328	VB	denotes	is
T1968	4329-4333	RB	denotes	also
T1969	4334-4337	RB	denotes	not
T1970	4338-4343	VB	denotes	known
T1971	4344-4351	IN	denotes	whether
T1972	4352-4357	DT	denotes	these
T1973	4358-4367	NN	denotes	processes
T1974	4368-4372	VB	denotes	rely
T1975	4373-4379	RB	denotes	solely
T1976	4380-4384	IN	denotes	upon
T1977	4385-4388	DT	denotes	the
T1978	4389-4399	NN	denotes	activation
T1979	4400-4402	IN	denotes	of
T1980	4403-4406	NN	denotes	PKA
T1981	4408-4417	RB	denotes	Therefore
T1982	4417-4418	-COMMA-	denotes	,
T1983	4419-4422	DT	denotes	the
T1984	4423-4432	NN	denotes	objective
T1985	4433-4435	IN	denotes	of
T1986	4436-4439	DT	denotes	the
T1987	4440-4447	JJ	denotes	present
T1988	4448-4453	NN	denotes	study
T1989	4454-4456	VB	denotes	is
T1990	4457-4459	TO	denotes	to
T1991	4460-4469	VB	denotes	determine
T1992	4470-4473	DT	denotes	the
T1993	4474-4481	NN	denotes	effects
T1994	4482-4484	IN	denotes	of
T1995	4485-4496	JJ	denotes	nonspecific
T1996	4497-4500	NN	denotes	PDE
T1997	4501-4511	NN	denotes	inhibition
T1998	4512-4516	IN	denotes	with
T1999	4517-4519	NN	denotes	1-
T2000	4519-4520	-LRB-	denotes	[
T2001	4520-4530	NN	denotes	5-oxohexyl
T2002	4530-4531	-RRB-	denotes	]
T2003	4531-4552	NN	denotes	-3,7-dimethylxanthine
T2004	4553-4554	-LRB-	denotes	(
T2005	4554-4568	NN	denotes	Pentoxifylline
T2006	4568-4569	-COLON-	denotes	;
T2007	4570-4573	NN	denotes	PTX
T2008	4573-4574	-RRB-	denotes	)
T2009	4575-4577	IN	denotes	on
T2010	4578-4583	NN	denotes	NF-κB
T2011	4584-4587	CC	denotes	and
T2012	4588-4592	NN	denotes	CREB
T2013	4593-4603	NN	denotes	activation
T2014	4604-4606	FW	denotes	in
T2015	4607-4612	FW	denotes	vitro
T2016	4613-4615	IN	denotes	in
T2017	4616-4621	JJ	denotes	human
T2018	4622-4633	JJ	denotes	mononuclear
T2019	4634-4639	NN	denotes	cells
T2020	4641-4645	IN	denotes	With
T2021	4646-4649	DT	denotes	the
T2022	4650-4653	NN	denotes	use
T2023	4654-4656	IN	denotes	of
T2024	4657-4665	JJ	denotes	specific
T2025	4666-4676	NN	denotes	inhibitors
T2026	4676-4677	-COMMA-	denotes	,
T2027	4678-4680	PRP	denotes	we
T2028	4681-4685	RB	denotes	also
T2029	4686-4698	VB	denotes	investigated
T2030	4699-4702	DT	denotes	the
T2031	4703-4707	NN	denotes	role
T2032	4708-4710	IN	denotes	of
T2033	4711-4714	NN	denotes	PKA
T2034	4715-4717	IN	denotes	in
T2035	4718-4729	JJ	denotes	LPS-induced
T2036	4730-4735	NN	denotes	TNF-α
T2037	4736-4746	NN	denotes	production
T2791	0-4	DT	denotes	this
T2792	5-10	NN	denotes	study
T2793	11-14	VB	denotes	was
T2794	4786-4794	VB	denotes	approved
T2795	4795-4797	IN	denotes	by
T2796	4798-4801	DT	denotes	the
T2797	4802-4807	NNP	denotes	Human
T2798	4808-4816	NNP	denotes	Research
T2799	4817-4828	NNP	denotes	Protections
T2800	4829-4836	NNP	denotes	Program
T2801	4837-4840	CC	denotes	and
T2802	4841-4844	DT	denotes	the
T2803	4845-4858	NNP	denotes	Institutional
T2804	4859-4865	NNP	denotes	Review
T2805	4866-4872	NNP	denotes	Board.
T2806	4873-4880	NNP	denotes	Written
T2807	4881-4888	NN	denotes	consent
T2808	4889-4891	TO	denotes	to
T2809	4892-4903	VB	denotes	participate
T2810	4904-4906	IN	denotes	in
T2811	4907-4910	DT	denotes	the
T2812	4911-4916	NN	denotes	study
T2813	4917-4920	VB	denotes	was
T2814	4921-4929	VB	denotes	obtained
T2815	4930-4934	IN	denotes	from
T2816	4935-4938	DT	denotes	all
T2817	4939-4949	NN	denotes	volunteers
T2818	4950-4955	RB	denotes	prior
T2819	4956-4958	TO	denotes	to
T2820	4959-4964	NN	denotes	blood
T2821	4965-4986	JJ	denotes	donation. Heparinized
T2822	4987-4998	NN	denotes	Vacutainers
T2823	4999-5003	VB	denotes	were
T2824	5004-5013	VB	denotes	purchased
T2825	5014-5018	IN	denotes	from
T2826	5019-5025	NNP	denotes	Becton
T2827	5026-5035	NNP	denotes	Dickinson
T2828	5036-5037	-LRB-	denotes	(
T2829	5037-5040	NNP	denotes	San
T2830	5041-5045	NNP	denotes	Jose
T2831	5045-5046	-COMMA-	denotes	,
T2832	5047-5049	NNP	denotes	CA
T2833	5049-5050	-RRB-	denotes	)
R369	T474	T473	arg1Of	study,this
R370	T477	T475	arg2Of	determine,was
R371	T477	T476	arg1Of	determine,to
R372	T479	T477	arg2Of	effect,determine
R373	T479	T478	arg1Of	effect,the
R374	T479	T480	arg1Of	effect,of
R375	T479	T484	arg1Of	effect,on
R376	T483	T480	arg2Of	inhibition,of
R377	T483	T481	arg1Of	inhibition,nonspecific
R378	T483	T482	arg1Of	inhibition,phosphodiesterase
R379	T487	T485	arg1Of	activation,transcription
R380	T487	T486	arg1Of	activation,factor
R381	T487	T488	arg1Of	activation,and
R382	T488	T484	arg2Of	and,on
R383	T488	T496	arg1Of	and,in
R384	T491	T489	arg1Of	factor-alpha,tumor
R385	T491	T490	arg1Of	factor-alpha,necrosis
R386	T491	T492	arg1Of	factor-alpha,(
R387	T493	T492	arg2Of	TNF-α,(
R388	T494	T492	arg3Of	),(
R389	T495	T488	arg2Of	production,and
R390	T495	T491	arg1Of	production,factor-alpha
R391	T499	T498	arg2Of	LPS,(
R392	T500	T498	arg3Of	),(
R393	T501	T497	arg1Of	-stimulated,lipopolysaccharide
R394	T501	T498	arg1Of	-stimulated,(
R395	T504	T496	arg2Of	cells,in
R396	T504	T501	arg1Of	cells,-stimulated
R397	T504	T502	arg1Of	cells,human
R398	T504	T503	arg1Of	cells,mononuclear
R399	T507	T506	arg1Of	production,The
R400	T507	T508	arg1Of	production,of
R401	T507	T510	arg1Of	production,following
R402	T507	T513	arg1Of	production,is
R403	T509	T508	arg2Of	TNF-α,of
R404	T512	T510	arg2Of	stimulation,following
R405	T512	T511	arg1Of	stimulation,LPS
R406	T514	T513	arg2Of	one,is
R407	T514	T515	arg1Of	one,of
R408	T518	T515	arg2Of	steps,of
R409	T518	T516	arg1Of	steps,the
R410	T518	T517	arg1Of	steps,key
R411	T518	T519	arg1Of	steps,in
R412	T521	T522	arg1Of	sepsis,and
R413	T522	T519	arg2Of	and,in
R414	T522	T520	arg1Of	and,bacterial
R415	T523	T522	arg2Of	inflammation,and
R416	T525	T524	arg1Of	mechanism,The
R417	T525	T526	arg2Of	mechanism,by
R418	T525	T527	arg1Of	mechanism,which
R419	T525	T538	arg1Of	mechanism,remains
R420	T525	T539	arg1Of	mechanism,unclear
R421	T529	T528	arg1Of	inhibition,phosphodiesterase
R422	T529	T530	arg1Of	inhibition,alters
R423	T530	T526	arg1Of	alters,by
R424	T530	T533	arg1Of	alters,in
R425	T532	T530	arg2Of	production,alters
R426	T532	T531	arg1Of	production,TNF-α
R427	T535	T533	arg2Of	presence,in
R428	T535	T534	arg1Of	presence,the
R429	T535	T536	arg1Of	presence,of
R430	T537	T536	arg2Of	LPS,of
R431	T539	T538	arg2Of	unclear,remains
R432	T543	T541	arg1Of	cells,Human
R433	T543	T542	arg1Of	cells,mononuclear
R434	T543	T544	arg1Of	cells,were
R435	T543	T545	arg2Of	cells,stimulated
R436	T545	T544	arg2Of	stimulated,were
R437	T545	T546	arg1Of	stimulated,with
R438	T547	T546	arg2Of	LPS,with
R439	T547	T548	arg1Of	LPS,(
R440	T547	T552	arg1Of	LPS,","
R441	T547	T553	arg1Of	LPS,in
R442	T547	T566	arg1Of	LPS,","
R443	T550	T548	arg2Of	μg/mL,(
R444	T550	T549	arg1Of	μg/mL,1
R445	T551	T548	arg3Of	),(
R446	T555	T556	arg1Of	presence,and
R447	T556	T553	arg2Of	and,in
R448	T556	T554	arg1Of	and,the
R449	T556	T558	arg1Of	and,of
R450	T557	T556	arg2Of	absence,and
R451	T559	T558	arg2Of	Pentoxifylline,of
R452	T559	T560	arg1Of	Pentoxifylline,(
R453	T561	T562	arg1Of	PTX,;
R454	T562	T560	arg2Of	;,(
R455	T564	T562	arg2Of	mM,;
R456	T564	T563	arg1Of	mM,20
R457	T565	T560	arg3Of	),(
R458	T570	T566	arg2Of	inhibitor,","
R459	T570	T567	arg1Of	inhibitor,a
R460	T570	T568	arg1Of	inhibitor,nonspecific
R461	T570	T569	arg1Of	inhibitor,phosphodiesterase
R462	T572	T571	arg1Of	blotting,Western
R463	T572	T573	arg1Of	blotting,of
R464	T572	T594	arg1Of	blotting,was
R465	T572	T595	arg2Of	blotting,performed
R466	T576	T574	arg2Of	I-κBα,phosphorylated
R467	T576	T575	arg1Of	I-κBα,cytoplasmic
R468	T576	T577	arg1Of	I-κBα,","
R469	T577	T585	arg1Of	",",and
R470	T580	T577	arg2Of	p65,","
R471	T580	T578	arg1Of	p65,nuclear
R472	T580	T579	arg1Of	p65,factor-κB
R473	T580	T581	arg1Of	p65,(
R474	T582	T581	arg2Of	NF-κB,(
R475	T583	T581	arg3Of	),(
R476	T585	T573	arg2Of	and,of
R477	T585	T584	arg1Of	and,","
R478	T590	T585	arg2Of	protein,and
R479	T590	T586	arg1Of	protein,nuclear
R480	T590	T587	arg1Of	protein,cAMP-response
R481	T590	T588	arg1Of	protein,element
R482	T590	T589	arg1Of	protein,binding
R483	T590	T591	arg1Of	protein,(
R484	T592	T591	arg2Of	CREB,(
R485	T593	T591	arg3Of	),(
R486	T595	T594	arg2Of	performed,was
R487	T597	T596	arg1Of	binding,DNA
R488	T597	T598	arg1Of	binding,of
R489	T597	T602	arg1Of	binding,was
R490	T597	T603	arg2Of	binding,verified
R491	T599	T600	arg1Of	NF-κB,and
R492	T600	T598	arg2Of	and,of
R493	T601	T600	arg2Of	CREB,and
R494	T603	T602	arg2Of	verified,was
R495	T608	T603	arg1Of	assay,verified
R496	T608	T604	arg2Of	assay,by
R497	T608	T605	arg1Of	assay,electrophoretic
R498	T608	T606	arg1Of	assay,mobility
R499	T608	T607	arg1Of	assay,shift
R500	T610	T609	arg1Of	levels,TNF-α
R501	T610	T611	arg1Of	levels,were
R502	T610	T612	arg2Of	levels,determined
R503	T612	T611	arg2Of	determined,were
R504	T612	T613	arg1Of	determined,in
R505	T612	T619	arg1Of	determined,in
R506	T615	T613	arg2Of	supernatant,in
R507	T615	T614	arg1Of	supernatant,the
R508	T615	T616	arg1Of	supernatant,of
R509	T618	T616	arg2Of	cells,of
R510	T618	T617	arg2Of	cells,stimulated
R511	T621	T622	arg1Of	presence,and
R512	T623	T622	arg2Of	absence,and
R513	T627	T619	arg2Of	inhibition,in
R514	T627	T620	arg1Of	inhibition,the
R515	T627	T621	arg1Of	inhibition,presence
R516	T627	T623	arg1Of	inhibition,absence
R517	T627	T624	arg1Of	inhibition,Protein
R518	T627	T625	arg1Of	inhibition,kinase
R519	T627	T626	arg1Of	inhibition,A
R520	T627	T628	arg1Of	inhibition,by
R521	T632	T628	arg2Of	assay,by
R522	T632	T629	arg1Of	assay,an
R523	T632	T630	arg1Of	assay,enzyme-linked
R524	T632	T631	arg1Of	assay,immunosorbent
R525	T632	T633	arg1Of	assay,(
R526	T634	T633	arg2Of	ELISA,(
R527	T635	T633	arg3Of	),(
R528	T637	T638	arg1Of	PTX,was
R529	T637	T639	arg2Of	PTX,demonstrated
R530	T637	T642	arg1Of	PTX,reduce
R531	T639	T638	arg2Of	demonstrated,was
R532	T642	T639	arg3Of	reduce,demonstrated
R533	T642	T640	arg1Of	reduce,to
R534	T642	T641	arg1Of	reduce,significantly
R535	T645	T643	arg1Of	phosphorylation,cytoplasmic
R536	T645	T644	arg1Of	phosphorylation,I-κBα
R537	T645	T646	arg1Of	phosphorylation,","
R538	T646	T651	arg1Of	",",and
R539	T649	T646	arg2Of	phosphorylation,","
R540	T649	T647	arg1Of	phosphorylation,nuclear
R541	T649	T648	arg1Of	phosphorylation,p65
R542	T651	T642	arg2Of	and,reduce
R543	T651	T650	arg1Of	and,","
R544	T655	T651	arg2Of	activity,and
R545	T655	T652	arg1Of	activity,the
R546	T655	T653	arg1Of	activity,DNA
R547	T655	T654	arg1Of	activity,binding
R548	T655	T656	arg1Of	activity,of
R549	T657	T656	arg2Of	NF-κB,of
R550	T659	T658	arg2Of	contrast,In
R551	T661	T663	arg1Of	PTX,enhanced
R552	T663	T658	arg1Of	enhanced,In
R553	T663	T660	arg1Of	enhanced,","
R554	T663	T662	arg1Of	enhanced,markedly
R555	T665	T666	arg1Of	phosphorylation,and
R556	T666	T663	arg2Of	and,enhanced
R557	T666	T664	arg1Of	and,the
R558	T666	T670	arg1Of	and,of
R559	T669	T666	arg2Of	activity,and
R560	T669	T667	arg1Of	activity,DNA
R561	T669	T668	arg1Of	activity,binding
R562	T671	T670	arg2Of	CREB,of
R563	T672	T674	arg2Of	Cells,treated
R564	T672	T679	arg1Of	Cells,secreted
R565	T674	T673	arg1Of	treated,concomitantly
R566	T674	T675	arg1Of	treated,with
R567	T676	T677	arg1Of	PTX,and
R568	T677	T675	arg2Of	and,with
R569	T678	T677	arg2Of	LPS,and
R570	T681	T679	arg2Of	levels,secreted
R571	T681	T680	arg1Of	levels,similar
R572	T681	T682	arg1Of	levels,of
R573	T681	T684	arg1Of	levels,in
R574	T683	T682	arg2Of	TNF-α,of
R575	T686	T687	arg1Of	presence,and
R576	T688	T687	arg2Of	absence,and
R577	T692	T684	arg2Of	inhibition,in
R578	T692	T685	arg1Of	inhibition,the
R579	T692	T686	arg1Of	inhibition,presence
R580	T692	T688	arg1Of	inhibition,absence
R581	T692	T689	arg1Of	inhibition,Protein
R582	T692	T690	arg1Of	inhibition,kinase
R583	T692	T691	arg1Of	inhibition,A
R584	T696	T694	arg1Of	level,The
R585	T696	T695	arg2Of	level,increased
R586	T696	T697	arg1Of	level,of
R587	T696	T699	arg1Of	level,that
R588	T696	T700	arg1Of	level,results
R589	T696	T704	arg1Of	level,affects
R590	T698	T697	arg2Of	cAMP,of
R591	T700	T701	arg1Of	results,from
R592	T703	T701	arg2Of	inhibition,from
R593	T703	T702	arg1Of	inhibition,phosphodiesterase
R594	T704	T709	arg1Of	affects,","
R595	T704	T710	modOf	affects,resulting
R596	T705	T706	arg1Of	cytoplasmic,and
R597	T707	T706	arg2Of	nuclear,and
R598	T708	T704	arg2Of	events,affects
R599	T708	T705	arg1Of	events,cytoplasmic
R600	T708	T707	arg1Of	events,nuclear
R601	T710	T711	arg1Of	resulting,in
R602	T710	T724	arg1Of	resulting,through
R603	T713	T712	arg1Of	attenuation,the
R604	T713	T714	arg1Of	attenuation,of
R605	T713	T716	arg1Of	attenuation,and
R606	T715	T714	arg2Of	NF-κB,of
R607	T716	T711	arg2Of	and,in
R608	T718	T716	arg2Of	activation,and
R609	T718	T717	arg1Of	activation,the
R610	T718	T719	arg1Of	activation,of
R611	T723	T719	arg2Of	binding,of
R612	T723	T720	arg1Of	binding,CREB
R613	T723	T721	arg1Of	binding,transcriptional
R614	T723	T722	arg1Of	binding,DNA
R615	T725	T724	arg2Of	pathways,through
R616	T725	T726	arg1Of	pathways,that
R617	T725	T727	arg1Of	pathways,are
R618	T725	T731	arg1Of	pathways,A-independent
R619	T727	T728	arg1Of	are,partially
R620	T731	T727	arg2Of	A-independent,are
R621	T731	T729	arg1Of	A-independent,Protein
R622	T731	T730	arg1Of	A-independent,kinase
R623	T735	T733	arg1Of	inhibition,PTX-mediated
R624	T735	T734	arg1Of	inhibition,phosphodiesterase
R625	T735	T736	arg1Of	inhibition,occurs
R626	T735	T745	arg1Of	inhibition,may
R627	T735	T746	arg1Of	inhibition,serve
R628	T736	T737	arg1Of	occurs,partially
R629	T736	T738	arg1Of	occurs,through
R630	T736	T744	arg1Of	occurs,and
R631	T743	T738	arg2Of	pathway,through
R632	T743	T739	arg1Of	pathway,a
R633	T743	T740	arg1Of	pathway,Protein
R634	T743	T741	arg1Of	pathway,kinase
R635	T743	T742	arg1Of	pathway,A-independent
R636	T746	T744	arg2Of	serve,and
R637	T746	T745	arg2Of	serve,may
R638	T746	T747	arg1Of	serve,as
R639	T750	T747	arg2Of	tool,as
R640	T750	T748	arg1Of	tool,a
R641	T750	T749	arg1Of	tool,useful
R642	T750	T751	arg1Of	tool,in
R643	T753	T751	arg2Of	attenuation,in
R644	T753	T752	arg1Of	attenuation,the
R645	T753	T754	arg1Of	attenuation,of
R646	T756	T754	arg2Of	inflammation,of
R647	T756	T755	arg1Of	inflammation,LPS-induced
R1298	T1616	T1612	arg1Of	lipopolysaccharide,The
R1299	T1616	T1613	arg1Of	lipopolysaccharide,bacterial
R1300	T1616	T1614	arg1Of	lipopolysaccharide,membrane
R1301	T1616	T1615	arg1Of	lipopolysaccharide,component
R1302	T1616	T1617	arg1Of	lipopolysaccharide,(
R1303	T1616	T1620	arg1Of	lipopolysaccharide,is
R1304	T1616	T1621	arg1Of	lipopolysaccharide,capable
R1305	T1616	T1623	arg1Of	lipopolysaccharide,initiating
R1306	T1618	T1617	arg2Of	LPS,(
R1307	T1619	T1617	arg3Of	),(
R1308	T1621	T1620	arg2Of	capable,is
R1309	T1621	T1622	arg1Of	capable,of
R1310	T1623	T1622	arg2Of	initiating,of
R1311	T1624	T1625	arg1Of	phosphorylation,and
R1312	T1625	T1623	arg2Of	and,initiating
R1313	T1625	T1627	arg1Of	and,of
R1314	T1626	T1625	arg2Of	activation,and
R1315	T1632	T1628	arg1Of	kinases,multiple
R1316	T1632	T1629	arg1Of	kinases,host
R1317	T1632	T1630	arg1Of	kinases,intracellular
R1318	T1632	T1631	arg1Of	kinases,protein
R1319	T1632	T1633	arg1Of	kinases,and
R1320	T1633	T1627	arg2Of	and,of
R1321	T1635	T1633	arg2Of	factors,and
R1322	T1635	T1634	arg1Of	factors,transcription
R1323	T1638	T1636	arg1Of	activation,Transcription
R1324	T1638	T1637	arg1Of	activation,factor
R1325	T1638	T1639	arg1Of	activation,","
R1326	T1638	T1640	arg1Of	activation,which
R1327	T1638	T1641	arg1Of	activation,results
R1328	T1638	T1652	arg1Of	activation,is
R1329	T1641	T1642	arg1Of	results,in
R1330	T1644	T1642	arg2Of	modulation,in
R1331	T1644	T1643	arg1Of	modulation,the
R1332	T1644	T1645	arg1Of	modulation,of
R1333	T1647	T1646	arg1Of	transcription,gene
R1334	T1647	T1648	arg1Of	transcription,and
R1335	T1648	T1645	arg2Of	and,of
R1336	T1650	T1648	arg2Of	synthesis,and
R1337	T1650	T1649	arg1Of	synthesis,protein
R1338	T1652	T1651	arg1Of	is,","
R1339	T1655	T1652	arg2Of	element,is
R1340	T1655	T1653	arg1Of	element,a
R1341	T1655	T1654	arg1Of	element,critical
R1342	T1655	T1656	arg1Of	element,in
R1343	T1659	T1656	arg2Of	mechanism,in
R1344	T1659	T1657	arg1Of	mechanism,the
R1345	T1659	T1658	arg1Of	mechanism,defense
R1346	T1659	T1660	arg1Of	mechanism,of
R1347	T1664	T1660	arg2Of	"system.1,2",of
R1348	T1664	T1661	arg1Of	"system.1,2",the
R1349	T1664	T1662	arg1Of	"system.1,2",host
R1350	T1664	T1663	arg1Of	"system.1,2",immune
R1351	T1668	T1665	arg1Of	activation,LPS-induced
R1352	T1668	T1666	arg1Of	activation,transcription
R1353	T1668	T1667	arg1Of	activation,factor
R1354	T1668	T1669	arg1Of	activation,has
R1355	T1668	T1670	arg1Of	activation,been
R1356	T1668	T1671	arg2Of	activation,shown
R1357	T1668	T1673	arg1Of	activation,be
R1358	T1671	T1669	arg2Of	shown,has
R1359	T1671	T1670	arg2Of	shown,been
R1360	T1673	T1671	arg3Of	be,shown
R1361	T1673	T1672	arg1Of	be,to
R1362	T1676	T1673	arg2Of	regulator,be
R1363	T1676	T1674	arg1Of	regulator,a
R1364	T1676	T1675	arg1Of	regulator,key
R1365	T1676	T1677	arg1Of	regulator,of
R1366	T1676	T1686	modOf	regulator,is
R1367	T1680	T1677	arg2Of	factor-α,of
R1368	T1680	T1678	arg1Of	factor-α,tumor
R1369	T1680	T1679	arg1Of	factor-α,necrosis
R1370	T1682	T1681	arg2Of	TNF-α,(
R1371	T1683	T1681	arg3Of	),(
R1372	T1685	T1681	arg1Of	TNF-α,(
R1373	T1685	T1684	arg1Of	TNF-α,production.3
R1374	T1685	T1686	arg1Of	TNF-α,is
R1375	T1690	T1686	arg2Of	cytokine,is
R1376	T1690	T1687	arg1Of	cytokine,a
R1377	T1690	T1688	arg1Of	cytokine,potent
R1378	T1690	T1689	arg1Of	cytokine,pro-inflammatory
R1379	T1690	T1691	arg2Of	cytokine,involved
R1380	T1691	T1692	arg1Of	involved,in
R1381	T1695	T1692	arg2Of	spectrum,in
R1382	T1695	T1693	arg1Of	spectrum,a
R1383	T1695	T1694	arg1Of	spectrum,wide
R1384	T1695	T1696	arg1Of	spectrum,of
R1385	T1698	T1696	arg2Of	responses,of
R1386	T1698	T1697	arg1Of	responses,cellular
R1387	T1702	T1701	arg1Of	synthesis,TNF-α
R1388	T1702	T1703	arg1Of	synthesis,can
R1389	T1702	T1704	arg1Of	synthesis,be
R1390	T1702	T1705	arg2Of	synthesis,attenuated
R1391	T1705	T1703	arg2Of	attenuated,can
R1392	T1705	T1704	arg2Of	attenuated,be
R1393	T1705	T1706	arg1Of	attenuated,in
R1394	T1705	T1716	arg1Of	attenuated,after
R1395	T1708	T1706	arg2Of	cells,in
R1396	T1708	T1707	arg1Of	cells,immune
R1397	T1708	T1709	arg2Of	cells,exposed
R1398	T1709	T1710	arg1Of	exposed,to
R1399	T1711	T1712	arg1Of	phosphodiesterase,(
R1400	T1713	T1712	arg2Of	PDE,(
R1401	T1714	T1712	arg3Of	),(
R1402	T1715	T1710	arg2Of	inhibition,to
R1403	T1715	T1711	arg1Of	inhibition,phosphodiesterase
R1404	T1717	T1716	arg2Of	challenge,after
R1405	T1717	T1718	arg1Of	challenge,by
R1406	T1720	T1718	arg2Of	variety,by
R1407	T1720	T1719	arg1Of	variety,a
R1408	T1720	T1721	arg1Of	variety,of
R1409	T1723	T1721	arg2Of	stimulants.4,of
R1410	T1723	T1722	arg1Of	stimulants.4,pro-inflammatory
R1411	T1726	T1724	arg1Of	mechanisms,The
R1412	T1726	T1725	arg1Of	mechanisms,signaling
R1413	T1726	T1727	arg2Of	mechanisms,affected
R1414	T1726	T1731	arg1Of	mechanisms,","
R1415	T1726	T1732	arg1Of	mechanisms,which
R1416	T1726	T1734	arg1Of	mechanisms,lead
R1417	T1726	T1742	arg1Of	mechanisms,have
R1418	T1726	T1744	arg1Of	mechanisms,been
R1419	T1726	T1746	arg2Of	mechanisms,characterized
R1420	T1730	T1727	arg1Of	inhibition,affected
R1421	T1730	T1728	arg2Of	inhibition,by
R1422	T1730	T1729	arg1Of	inhibition,PDE
R1423	T1734	T1733	arg1Of	lead,ultimately
R1424	T1734	T1735	arg1Of	lead,to
R1425	T1737	T1735	arg2Of	downregulation,to
R1426	T1737	T1736	arg1Of	downregulation,the
R1427	T1737	T1738	arg1Of	downregulation,of
R1428	T1740	T1738	arg2Of	production,of
R1429	T1740	T1739	arg1Of	production,TNF-α
R1430	T1746	T1699	arg1Of	characterized,Furthermore
R1431	T1746	T1700	arg1Of	characterized,","
R1432	T1746	T1703	modOf	characterized,can
R1433	T1746	T1741	arg1Of	characterized,","
R1434	T1746	T1742	arg2Of	characterized,have
R1435	T1746	T1743	arg1Of	characterized,not
R1436	T1746	T1744	arg2Of	characterized,been
R1437	T1746	T1745	arg1Of	characterized,well
R1438	T1746	T1747	arg1Of	characterized,in
R1439	T1749	T1747	arg2Of	cells,in
R1440	T1749	T1748	arg1Of	cells,inflammatory
R1441	T1752	T1753	arg1Of	it,has
R1442	T1752	T1754	arg1Of	it,been
R1443	T1752	T1755	arg2Of	it,demonstrated
R1444	T1755	T1750	arg1Of	demonstrated,Classically
R1445	T1755	T1751	arg1Of	demonstrated,","
R1446	T1755	T1753	arg2Of	demonstrated,has
R1447	T1755	T1754	arg2Of	demonstrated,been
R1448	T1758	T1757	arg1Of	inhibition,PDE
R1449	T1758	T1759	arg1Of	inhibition,results
R1450	T1759	T1760	arg1Of	results,in
R1451	T1759	T1773	arg1Of	results,and
R1452	T1763	T1760	arg2Of	accumulation,in
R1453	T1763	T1761	arg1Of	accumulation,the
R1454	T1763	T1762	arg1Of	accumulation,intracellular
R1455	T1763	T1764	arg1Of	accumulation,of
R1456	T1769	T1764	arg2Of	"adenosine-3,5-monophosphate",of
R1457	T1769	T1765	arg1Of	"adenosine-3,5-monophosphate",the
R1458	T1769	T1766	arg1Of	"adenosine-3,5-monophosphate",second
R1459	T1769	T1767	arg1Of	"adenosine-3,5-monophosphate",messenger
R1460	T1769	T1768	arg1Of	"adenosine-3,5-monophosphate",cyclic
R1461	T1769	T1770	arg1Of	"adenosine-3,5-monophosphate",(
R1462	T1771	T1770	arg2Of	cAMP,(
R1463	T1772	T1770	arg3Of	),(
R1464	T1773	T1810	arg1Of	and,and
R1465	T1775	T1774	arg1Of	activation,subsequent
R1466	T1775	T1776	arg1Of	activation,of
R1467	T1775	T1787	arg1Of	activation,leads
R1468	T1781	T1780	arg2Of	PKA,(
R1469	T1782	T1780	arg3Of	),(
R1470	T1785	T1776	arg2Of	activation,of
R1471	T1785	T1777	arg1Of	activation,Protein
R1472	T1785	T1778	arg1Of	activation,kinase
R1473	T1785	T1779	arg1Of	activation,A
R1474	T1785	T1780	arg1Of	activation,(
R1475	T1785	T1783	arg1Of	activation,.5
R1476	T1785	T1784	arg1Of	activation,PKA
R1477	T1787	T1773	arg2Of	leads,and
R1478	T1787	T1786	arg1Of	leads,then
R1479	T1787	T1788	arg1Of	leads,to
R1480	T1790	T1788	arg2Of	phosphorylation,to
R1481	T1790	T1789	arg1Of	phosphorylation,the
R1482	T1790	T1791	arg1Of	phosphorylation,of
R1483	T1798	T1793	arg1Of	protein,transcription
R1484	T1798	T1794	arg1Of	protein,factor
R1485	T1798	T1795	arg1Of	protein,cAMP-response
R1486	T1798	T1796	arg1Of	protein,element
R1487	T1798	T1797	arg1Of	protein,binding
R1488	T1798	T1799	arg1Of	protein,(
R1489	T1798	T1802	arg1Of	protein,","
R1490	T1800	T1799	arg2Of	CREB,(
R1491	T1801	T1799	arg3Of	),(
R1492	T1802	T1791	arg2Of	",",of
R1493	T1802	T1792	arg1Of	",",the
R1494	T1803	T1802	arg2Of	transmission,","
R1495	T1803	T1804	arg1Of	transmission,of
R1496	T1803	T1806	arg1Of	transmission,into
R1497	T1805	T1804	arg2Of	signals,of
R1498	T1808	T1806	arg2Of	nucleus,into
R1499	T1808	T1807	arg1Of	nucleus,the
R1500	T1810	T1755	arg3Of	and,demonstrated
R1501	T1810	T1756	arg1Of	and,that
R1502	T1810	T1809	arg1Of	and,","
R1503	T1813	T1811	arg1Of	modulation,the
R1504	T1813	T1812	arg1Of	modulation,subsequent
R1505	T1813	T1814	arg1Of	modulation,of
R1506	T1813	T1822	arg1Of	modulation,does
R1507	T1813	T1825	arg1Of	modulation,explain
R1508	T1816	T1815	arg1Of	transcription.6,gene
R1509	T1819	T1818	arg1Of	simple,apparently
R1510	T1821	T1814	arg2Of	cascade,of
R1511	T1821	T1816	arg1Of	cascade,transcription.6
R1512	T1821	T1817	arg1Of	cascade,This
R1513	T1821	T1819	arg1Of	cascade,simple
R1514	T1821	T1820	arg1Of	cascade,linear
R1515	T1825	T1810	arg2Of	explain,and
R1516	T1825	T1822	arg2Of	explain,does
R1517	T1825	T1823	arg1Of	explain,not
R1518	T1825	T1824	arg1Of	explain,fully
R1519	T1827	T1825	arg2Of	mechanism,explain
R1520	T1827	T1826	arg1Of	mechanism,the
R1521	T1827	T1828	arg2Of	mechanism,by
R1522	T1827	T1829	arg1Of	mechanism,which
R1523	T1831	T1830	arg1Of	elevation,an
R1524	T1831	T1832	arg1Of	elevation,in
R1525	T1831	T1837	arg1Of	elevation,exerts
R1526	T1836	T1832	arg2Of	level,in
R1527	T1836	T1833	arg1Of	level,the
R1528	T1836	T1834	arg1Of	level,intracellular
R1529	T1836	T1835	arg1Of	level,cAMP
R1530	T1837	T1828	arg1Of	exerts,by
R1531	T1837	T1840	arg1Of	exerts,on
R1532	T1839	T1837	arg2Of	effects,exerts
R1533	T1839	T1838	arg1Of	effects,wide-ranging
R1534	T1843	T1840	arg2Of	functions,on
R1535	T1843	T1841	arg1Of	functions,multiple
R1536	T1843	T1842	arg1Of	functions,cellular
R1537	T1844	T1845	arg1Of	There,is
R1538	T1848	T1845	arg2Of	body,is
R1539	T1848	T1846	arg1Of	body,a
R1540	T1848	T1847	arg1Of	body,growing
R1541	T1848	T1849	arg1Of	body,of
R1542	T1848	T1851	arg1Of	body,suggesting
R1543	T1850	T1849	arg2Of	evidence,of
R1544	T1853	T1854	arg1Of	cAMP,may
R1545	T1853	T1855	arg1Of	cAMP,function
R1546	T1855	T1851	arg2Of	function,suggesting
R1547	T1855	T1852	arg1Of	function,that
R1548	T1855	T1854	arg2Of	function,may
R1549	T1855	T1856	arg1Of	function,through
R1550	T1858	T1859	arg1Of	PKA-dependent,and
R1551	T1860	T1859	arg2Of	-independent,and
R1552	T1861	T1856	arg2Of	mechanisms.6–8,through
R1553	T1861	T1857	arg1Of	mechanisms.6–8,both
R1554	T1861	T1858	arg1Of	mechanisms.6–8,PKA-dependent
R1555	T1861	T1860	arg1Of	mechanisms.6–8,-independent
R1556	T1863	T1862	arg1Of	activation,LPS-induced
R1557	T1863	T1864	arg1Of	activation,of
R1558	T1863	T1873	arg1Of	activation,has
R1559	T1863	T1875	arg1Of	activation,been
R1560	T1869	T1864	arg2Of	factor-κB,of
R1561	T1869	T1865	arg1Of	factor-κB,the
R1562	T1869	T1866	arg1Of	factor-κB,transcription
R1563	T1869	T1867	arg1Of	factor-κB,factor
R1564	T1869	T1868	arg1Of	factor-κB,nuclear
R1565	T1869	T1870	arg1Of	factor-κB,(
R1566	T1871	T1870	arg2Of	NF-κB,(
R1567	T1872	T1870	arg3Of	),(
R1568	T1875	T1873	arg2Of	been,has
R1569	T1875	T1874	arg1Of	been,also
R1570	T1877	T1875	arg2Of	focus,been
R1571	T1877	T1876	arg1Of	focus,the
R1572	T1877	T1878	arg1Of	focus,of
R1573	T1881	T1878	arg2Of	deal,of
R1574	T1881	T1879	arg1Of	deal,a
R1575	T1881	T1880	arg1Of	deal,great
R1576	T1881	T1882	arg1Of	deal,of
R1577	T1883	T1882	arg2Of	research,of
R1578	T1884	T1885	arg1Of	It,has
R1579	T1884	T1886	arg1Of	It,been
R1580	T1884	T1888	arg2Of	It,demonstrated
R1581	T1888	T1885	arg2Of	demonstrated,has
R1582	T1888	T1886	arg2Of	demonstrated,been
R1583	T1888	T1887	arg1Of	demonstrated,clearly
R1584	T1890	T1891	arg1Of	agents,that
R1585	T1890	T1892	arg1Of	agents,increase
R1586	T1890	T1896	arg1Of	agents,inhibit
R1587	T1894	T1892	arg2Of	cAMP,increase
R1588	T1894	T1893	arg1Of	cAMP,intracellular
R1589	T1896	T1888	arg3Of	inhibit,demonstrated
R1590	T1896	T1889	arg1Of	inhibit,that
R1591	T1896	T1895	arg1Of	inhibit,also
R1592	T1900	T1896	arg2Of	transcription,inhibit
R1593	T1900	T1897	arg1Of	transcription,NF-κB-dependent
R1594	T1900	T1898	arg1Of	transcription,pro-inflammatory
R1595	T1900	T1899	arg1Of	transcription,gene
R1596	T1900	T1901	arg1Of	transcription,","
R1597	T1900	T1903	arg1Of	transcription,of
R1598	T1903	T1902	arg1Of	of,particularly
R1599	T1906	T1903	arg2Of	gene.9,of
R1600	T1906	T1904	arg1Of	gene.9,the
R1601	T1906	T1905	arg1Of	gene.9,TNF-α
R1602	T1907	T1908	arg1Of	Controversy,exists
R1603	T1908	T1909	arg1Of	exists,regarding
R1604	T1912	T1909	arg2Of	mechanism,regarding
R1605	T1912	T1910	arg1Of	mechanism,the
R1606	T1912	T1911	arg1Of	mechanism,exact
R1607	T1912	T1913	arg1Of	mechanism,(
R1608	T1912	T1916	arg2Of	mechanism,by
R1609	T1912	T1917	arg1Of	mechanism,which
R1610	T1914	T1913	arg2Of	s,(
R1611	T1915	T1913	arg3Of	),(
R1612	T1919	T1918	arg1Of	inhibition,PDE
R1613	T1919	T1920	arg1Of	inhibition,down-regulates
R1614	T1920	T1916	arg1Of	down-regulates,by
R1615	T1922	T1920	arg2Of	production,down-regulates
R1616	T1922	T1921	arg1Of	production,TNF-α
R1617	T1923	T1924	arg1Of	Possibilities,include
R1618	T1923	T1927	arg1Of	Possibilities,are
R1619	T1923	T1929	arg2Of	Possibilities,limited
R1620	T1924	T1926	arg1Of	include,but
R1621	T1926	T1925	arg1Of	but,","
R1622	T1926	T1931	arg1Of	but,","
R1623	T1929	T1926	arg2Of	limited,but
R1624	T1929	T1927	arg2Of	limited,are
R1625	T1929	T1928	arg1Of	limited,not
R1626	T1929	T1930	arg1Of	limited,to
R1627	T1932	T1933	arg1Of	inhibition,of
R1628	T1932	T1945	arg1Of	inhibition,increased
R1629	T1937	T1934	arg1Of	activity,NF-κB
R1630	T1937	T1935	arg1Of	activity,DNA
R1631	T1937	T1936	arg1Of	activity,binding
R1632	T1937	T1938	arg1Of	activity,","
R1633	T1938	T1933	arg2Of	",",of
R1634	T1939	T1938	arg2Of	downregulation,","
R1635	T1939	T1940	arg1Of	downregulation,of
R1636	T1943	T1940	arg2Of	activity,of
R1637	T1943	T1941	arg1Of	activity,NF-κB
R1638	T1943	T1942	arg1Of	activity,transcriptional
R1639	T1945	T1931	arg2Of	increased,","
R1640	T1945	T1944	arg1Of	increased,","
R1641	T1947	T1946	arg1Of	activation,CREB
R1642	T1947	T1949	arg1Of	activation,and
R1643	T1949	T1945	arg2Of	and,increased
R1644	T1949	T1948	arg1Of	and,","
R1645	T1950	T1949	arg2Of	competition,and
R1646	T1950	T1951	arg1Of	competition,between
R1647	T1952	T1953	arg1Of	NF-κB,and
R1648	T1953	T1951	arg2Of	and,between
R1649	T1953	T1955	arg1Of	and,for
R1650	T1954	T1953	arg2Of	CREB,and
R1651	T1957	T1955	arg2Of	co-activators,for
R1652	T1957	T1956	arg1Of	co-activators,common
R1653	T1957	T1959	arg1Of	co-activators,as
R1654	T1959	T1958	arg1Of	as,such
R1655	T1962	T1959	arg2Of	protein,as
R1656	T1962	T1960	arg1Of	protein,CREB
R1657	T1962	T1961	arg1Of	protein,binding
R1658	T1962	T1963	arg1Of	protein,(
R1659	T1964	T1963	arg2Of	CBP,(
R1660	T1965	T1963	arg3Of	),(
R1661	T1966	T1967	arg1Of	It,is
R1662	T1966	T1970	arg2Of	It,known
R1663	T1970	T1967	arg2Of	known,is
R1664	T1970	T1968	arg1Of	known,also
R1665	T1970	T1969	arg1Of	known,not
R1666	T1973	T1972	arg1Of	processes,these
R1667	T1973	T1974	arg1Of	processes,rely
R1668	T1974	T1970	arg3Of	rely,known
R1669	T1974	T1971	arg1Of	rely,whether
R1670	T1974	T1975	arg1Of	rely,solely
R1671	T1974	T1976	arg1Of	rely,upon
R1672	T1978	T1976	arg2Of	activation,upon
R1673	T1978	T1977	arg1Of	activation,the
R1674	T1978	T1979	arg1Of	activation,of
R1675	T1980	T1979	arg2Of	PKA,of
R1676	T1984	T1983	arg1Of	objective,the
R1677	T1984	T1985	arg1Of	objective,of
R1678	T1984	T1989	arg1Of	objective,is
R1679	T1988	T1985	arg2Of	study,of
R1680	T1988	T1986	arg1Of	study,the
R1681	T1988	T1987	arg1Of	study,present
R1682	T1989	T1981	arg1Of	is,Therefore
R1683	T1989	T1982	arg1Of	is,","
R1684	T1991	T1989	arg2Of	determine,is
R1685	T1991	T1990	arg1Of	determine,to
R1686	T1991	T1998	arg1Of	determine,with
R1687	T1991	T2015	arg1Of	determine,vitro
R1688	T1991	T2016	arg1Of	determine,in
R1689	T1993	T1991	arg2Of	effects,determine
R1690	T1993	T1992	arg1Of	effects,the
R1691	T1993	T1994	arg1Of	effects,of
R1692	T1997	T1994	arg2Of	inhibition,of
R1693	T1997	T1995	arg1Of	inhibition,nonspecific
R1694	T1997	T1996	arg1Of	inhibition,PDE
R1695	T2001	T2000	arg2Of	5-oxohexyl,[
R1696	T2002	T2000	arg3Of	],[
R1697	T2003	T1998	arg2Of	"-3,7-dimethylxanthine",with
R1698	T2003	T1999	arg1Of	"-3,7-dimethylxanthine",1-
R1699	T2003	T2000	arg1Of	"-3,7-dimethylxanthine",[
R1700	T2003	T2004	arg1Of	"-3,7-dimethylxanthine",(
R1701	T2003	T2009	arg1Of	"-3,7-dimethylxanthine",on
R1702	T2005	T2004	arg2Of	Pentoxifylline,(
R1703	T2005	T2006	arg1Of	Pentoxifylline,;
R1704	T2007	T2006	arg2Of	PTX,;
R1705	T2008	T2004	arg3Of	),(
R1706	T2010	T2011	arg1Of	NF-κB,and
R1707	T2012	T2011	arg2Of	CREB,and
R1708	T2013	T2009	arg2Of	activation,on
R1709	T2013	T2010	arg1Of	activation,NF-κB
R1710	T2013	T2012	arg1Of	activation,CREB
R1711	T2015	T2014	arg1Of	vitro,in
R1712	T2019	T2016	arg2Of	cells,in
R1713	T2019	T2017	arg1Of	cells,human
R1714	T2019	T2018	arg1Of	cells,mononuclear
R1715	T2022	T2020	arg2Of	use,With
R1716	T2022	T2021	arg1Of	use,the
R1717	T2022	T2023	arg1Of	use,of
R1718	T2025	T2023	arg2Of	inhibitors,of
R1719	T2025	T2024	arg1Of	inhibitors,specific
R1720	T2027	T2029	arg1Of	we,investigated
R1721	T2029	T2020	arg1Of	investigated,With
R1722	T2029	T2026	arg1Of	investigated,","
R1723	T2029	T2028	arg1Of	investigated,also
R1724	T2031	T2029	arg2Of	role,investigated
R1725	T2031	T2030	arg1Of	role,the
R1726	T2031	T2032	arg1Of	role,of
R1727	T2031	T2034	arg1Of	role,in
R1728	T2033	T2032	arg2Of	PKA,of
R1729	T2037	T2034	arg2Of	production,in
R1730	T2037	T2035	arg1Of	production,LPS-induced
R1731	T2037	T2036	arg1Of	production,TNF-α
R2218	T2792	T2791	arg1Of	study,this
R2219	T2792	T2793	arg1Of	study,was
R2220	T2792	T2794	arg2Of	study,approved
R2221	T2794	T2793	arg2Of	approved,was
R2222	T2794	T2810	arg1Of	approved,in
R2223	T2800	T2796	arg1Of	Program,the
R2224	T2800	T2797	arg1Of	Program,Human
R2225	T2800	T2798	arg1Of	Program,Research
R2226	T2800	T2799	arg1Of	Program,Protections
R2227	T2800	T2801	arg1Of	Program,and
R2228	T2801	T2794	arg1Of	and,approved
R2229	T2801	T2795	arg2Of	and,by
R2230	T2806	T2803	arg1Of	Written,Institutional
R2231	T2806	T2804	arg1Of	Written,Review
R2232	T2806	T2805	arg1Of	Written,Board.
R2233	T2807	T2801	arg2Of	consent,and
R2234	T2807	T2802	arg1Of	consent,the
R2235	T2807	T2806	arg1Of	consent,Written
R2236	T2807	T2808	modOf	consent,to
R2237	T2809	T2808	arg1Of	participate,to
R2238	T2812	T2811	arg1Of	study,the
R2239	T2812	T2813	arg1Of	study,was
R2240	T2812	T2814	arg2Of	study,obtained
R2241	T2814	T2813	arg2Of	obtained,was
R2242	T2814	T2815	arg1Of	obtained,from
R2243	T2814	T2818	arg1Of	obtained,prior
R2244	T2817	T2815	arg2Of	volunteers,from
R2245	T2817	T2816	arg1Of	volunteers,all
R2246	T2818	T2819	arg1Of	prior,to
R2247	T2820	T2819	arg2Of	blood,to
R2248	T2822	T2821	arg1Of	Vacutainers,"donation. Heparinized"
R2249	T2822	T2823	arg1Of	Vacutainers,were
R2250	T2822	T2824	arg2Of	Vacutainers,purchased
R2251	T2824	T2810	arg2Of	purchased,in
R2252	T2824	T2813	modOf	purchased,was
R2253	T2824	T2823	arg2Of	purchased,were
R2254	T2824	T2825	arg1Of	purchased,from
R2255	T2827	T2825	arg2Of	Dickinson,from
R2256	T2827	T2826	arg1Of	Dickinson,Becton
R2257	T2827	T2828	arg1Of	Dickinson,(
R2258	T2830	T2828	arg2Of	Jose,(
R2259	T2830	T2829	arg1Of	Jose,San
R2260	T2830	T2831	arg1Of	Jose,","
R2261	T2832	T2831	arg2Of	CA,","
R2262	T2833	T2828	arg3Of	),(

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T162	72-82	NN	denotes	inhibition
T163	83-85	IN	denotes	on
T164	86-99	NN	denotes	transcription
T165	100-106	NN	denotes	factor
T166	107-117	NN	denotes	activation
T167	118-121	CC	denotes	and
T168	122-127	NN	denotes	tumor
T169	128-136	NN	denotes	necrosis
T170	137-149	NN	denotes	factor-alpha
T171	150-151	-LRB-	denotes	(
T172	151-156	NNP	denotes	TNF-α
T173	156-157	-RRB-	denotes	)
T174	158-168	NN	denotes	production
T175	169-171	IN	denotes	in
T176	172-190	NN	denotes	lipopolysaccharide
T177	191-192	-LRB-	denotes	(
T178	192-195	NNP	denotes	LPS
T179	195-196	-RRB-	denotes	)
T180	196-197	:	denotes	-
T181	197-207	VBN	denotes	stimulated
T182	208-213	JJ	denotes	human
T183	214-225	NN	denotes	mononuclear
T184	226-231	NNS	denotes	cells
T185	231-232	.	denotes	.
T186	234-246	NNP	denotes	INTRODUCTION
T187	247-250	DT	denotes	The
T188	251-261	NN	denotes	production
T189	262-264	IN	denotes	of
T190	265-270	NNP	denotes	TNF-α
T191	271-280	VBG	denotes	following
T192	281-284	NNP	denotes	LPS
T193	285-296	NN	denotes	stimulation
T194	297-299	VBZ	denotes	is
T195	300-303	CD	denotes	one
T196	304-306	IN	denotes	of
T197	307-310	DT	denotes	the
T198	311-314	JJ	denotes	key
T199	315-320	NNS	denotes	steps
T200	321-323	IN	denotes	in
T201	324-333	JJ	denotes	bacterial
T202	334-340	NN	denotes	sepsis
T203	341-344	CC	denotes	and
T204	345-357	NN	denotes	inflammation
T205	357-358	.	denotes	.
T206	359-362	DT	denotes	The
T207	363-372	NN	denotes	mechanism
T208	373-375	IN	denotes	by
T209	376-381	WDT	denotes	which
T210	382-399	NN	denotes	phosphodiesterase
T211	400-410	NN	denotes	inhibition
T212	411-417	VBZ	denotes	alters
T213	418-423	JJ	denotes	TNF-α
T214	424-434	NN	denotes	production
T215	435-437	IN	denotes	in
T216	438-441	DT	denotes	the
T217	442-450	NN	denotes	presence
T218	451-453	IN	denotes	of
T219	454-457	NNP	denotes	LPS
T220	458-465	VBZ	denotes	remains
T221	466-473	JJ	denotes	unclear
T222	473-474	.	denotes	.
T223	476-483	NNP	denotes	METHODS
T224	484-489	NNP	denotes	Human
T225	490-501	NN	denotes	mononuclear
T226	502-507	NNS	denotes	cells
T227	508-512	VBD	denotes	were
T228	513-523	VBN	denotes	stimulated
T229	524-528	IN	denotes	with
T230	529-532	NNP	denotes	LPS
T231	533-534	-LRB-	denotes	(
T232	534-535	CD	denotes	1
T233	536-538	NN	denotes	μg
T234	538-539	NN	denotes	/
T235	539-541	NNP	denotes	mL
T236	541-542	-RRB-	denotes	)
T237	542-543	,	denotes	,
T238	544-546	IN	denotes	in
T239	547-550	DT	denotes	the
T240	551-559	NN	denotes	presence
T241	560-563	CC	denotes	and
T242	564-571	NN	denotes	absence
T243	572-574	IN	denotes	of
T244	575-589	NNP	denotes	Pentoxifylline
T245	590-591	-LRB-	denotes	(
T246	591-594	NNP	denotes	PTX
T247	594-595	:	denotes	;
T248	596-598	CD	denotes	20
T249	599-601	NNP	denotes	mM
T250	601-602	-RRB-	denotes	)
T251	602-603	,	denotes	,
T252	604-605	DT	denotes	a
T253	606-617	JJ	denotes	nonspecific
T254	618-635	NN	denotes	phosphodiesterase
T255	636-645	NN	denotes	inhibitor
T256	645-646	.	denotes	.
T257	647-654	JJ	denotes	Western
T258	655-663	VBG	denotes	blotting
T259	664-666	IN	denotes	of
T260	667-681	VBN	denotes	phosphorylated
T261	682-693	JJ	denotes	cytoplasmic
T262	694-699	NNP	denotes	I-κBα
T263	699-700	,	denotes	,
T264	701-708	JJ	denotes	nuclear
T265	709-718	NNP	denotes	factor-κB
T266	719-722	NNS	denotes	p65
T267	723-724	-LRB-	denotes	(
T268	724-729	NN	denotes	NF-κB
T269	729-730	-RRB-	denotes	)
T270	730-731	,	denotes	,
T271	732-735	CC	denotes	and
T272	736-743	JJ	denotes	nuclear
T273	744-757	JJ	denotes	cAMP-response
T274	758-765	NN	denotes	element
T275	766-773	JJ	denotes	binding
T276	774-781	NN	denotes	protein
T277	782-783	-LRB-	denotes	(
T278	783-787	NNP	denotes	CREB
T279	787-788	-RRB-	denotes	)
T280	789-792	VBD	denotes	was
T281	793-802	VBN	denotes	performed
T282	802-803	.	denotes	.
T283	804-807	NNP	denotes	DNA
T284	808-815	JJ	denotes	binding
T285	816-818	IN	denotes	of
T286	819-824	NNP	denotes	NF-κB
T287	825-828	CC	denotes	and
T288	829-833	NNP	denotes	CREB
T289	834-837	VBD	denotes	was
T290	838-846	VBN	denotes	verified
T291	847-849	IN	denotes	by
T292	850-865	JJ	denotes	electrophoretic
T293	866-874	NN	denotes	mobility
T294	875-880	NN	denotes	shift
T295	881-886	NN	denotes	assay
T296	886-887	.	denotes	.
T297	888-893	JJ	denotes	TNF-α
T298	894-900	NNS	denotes	levels
T299	901-905	VBD	denotes	were
T300	906-916	VBN	denotes	determined
T301	917-919	IN	denotes	in
T302	920-923	DT	denotes	the
T303	924-935	NN	denotes	supernatant
T304	936-938	IN	denotes	of
T305	939-949	VBN	denotes	stimulated
T306	950-955	NNS	denotes	cells
T307	956-958	IN	denotes	in
T308	959-962	DT	denotes	the
T309	963-971	NN	denotes	presence
T310	972-975	CC	denotes	and
T311	976-983	NN	denotes	absence
T312	984-991	NNP	denotes	Protein
T313	992-998	NN	denotes	kinase
T314	999-1000	DT	denotes	A
T315	1001-1011	NN	denotes	inhibition
T316	1012-1014	IN	denotes	by
T317	1015-1017	DT	denotes	an
T318	1018-1031	JJ	denotes	enzyme-linked
T319	1032-1045	JJ	denotes	immunosorbent
T320	1046-1051	NN	denotes	assay
T321	1052-1053	-LRB-	denotes	(
T322	1053-1058	NNP	denotes	ELISA
T323	1058-1059	-RRB-	denotes	)
T324	1059-1060	.	denotes	.
T325	1062-1069	NNS	denotes	RESULTS
T326	1070-1073	VBP	denotes	PTX
T327	1074-1077	VBD	denotes	was
T328	1078-1090	VBN	denotes	demonstrated
T329	1091-1093	TO	denotes	to
T330	1094-1107	RB	denotes	significantly
T331	1108-1114	VB	denotes	reduce
T332	1115-1126	JJ	denotes	cytoplasmic
T333	1127-1132	JJ	denotes	I-κBα
T334	1133-1148	NN	denotes	phosphorylation
T335	1148-1149	,	denotes	,
T336	1150-1157	JJ	denotes	nuclear
T337	1158-1161	NNS	denotes	p65
T338	1162-1177	NN	denotes	phosphorylation
T339	1177-1178	,	denotes	,
T340	1179-1182	CC	denotes	and
T341	1183-1186	DT	denotes	the
T342	1187-1190	NNP	denotes	DNA
T343	1191-1198	JJ	denotes	binding
T344	1199-1207	NN	denotes	activity
T345	1208-1210	IN	denotes	of
T346	1211-1216	NN	denotes	NF-κB
T347	1216-1217	.	denotes	.
T348	1218-1220	IN	denotes	In
T349	1221-1229	NN	denotes	contrast
T350	1229-1230	,	denotes	,
T351	1231-1234	NNP	denotes	PTX
T352	1235-1243	RB	denotes	markedly
T353	1244-1252	VBD	denotes	enhanced
T354	1253-1256	DT	denotes	the
T355	1257-1272	NN	denotes	phosphorylation
T356	1273-1276	CC	denotes	and
T357	1277-1280	NN	denotes	DNA
T358	1281-1288	JJ	denotes	binding
T359	1289-1297	NN	denotes	activity
T360	1298-1300	IN	denotes	of
T361	1301-1305	NNP	denotes	CREB
T362	1305-1306	.	denotes	.
T363	1307-1312	NNS	denotes	Cells
T364	1313-1326	RB	denotes	concomitantly
T365	1327-1334	VBN	denotes	treated
T366	1335-1339	IN	denotes	with
T367	1340-1343	NNP	denotes	PTX
T368	1344-1347	CC	denotes	and
T369	1348-1351	NNP	denotes	LPS
T370	1352-1360	VBD	denotes	secreted
T371	1361-1368	JJ	denotes	similar
T372	1369-1375	NNS	denotes	levels
T373	1376-1378	IN	denotes	of
T374	1379-1384	NNP	denotes	TNF-α
T375	1385-1387	IN	denotes	in
T376	1388-1391	DT	denotes	the
T377	1392-1400	NN	denotes	presence
T378	1401-1404	CC	denotes	and
T379	1405-1412	NN	denotes	absence
T380	1413-1420	NNP	denotes	Protein
T381	1421-1427	NN	denotes	kinase
T382	1428-1429	DT	denotes	A
T383	1430-1440	NN	denotes	inhibition
T384	1440-1441	.	denotes	.
T385	1443-1453	NNP	denotes	DISCUSSION
T386	1454-1457	NNP	denotes	The
T387	1458-1467	VBD	denotes	increased
T388	1468-1473	NN	denotes	level
T389	1474-1476	IN	denotes	of
T390	1477-1481	NNP	denotes	cAMP
T391	1482-1486	WDT	denotes	that
T392	1487-1494	VBZ	denotes	results
T393	1495-1499	IN	denotes	from
T394	1500-1517	NN	denotes	phosphodiesterase
T395	1518-1528	NN	denotes	inhibition
T396	1529-1536	VBZ	denotes	affects
T397	1537-1548	JJ	denotes	cytoplasmic
T398	1549-1552	CC	denotes	and
T399	1553-1560	JJ	denotes	nuclear
T400	1561-1567	NNS	denotes	events
T401	1567-1568	,	denotes	,
T402	1569-1578	VBG	denotes	resulting
T403	1579-1581	IN	denotes	in
T404	1582-1585	DT	denotes	the
T405	1586-1597	NN	denotes	attenuation
T406	1598-1600	IN	denotes	of
T407	1601-1606	NNP	denotes	NF-κB
T408	1607-1610	CC	denotes	and
T409	1611-1614	DT	denotes	the
T410	1615-1625	NN	denotes	activation
T411	1626-1628	IN	denotes	of
T412	1629-1633	NNP	denotes	CREB
T413	1634-1649	JJ	denotes	transcriptional
T414	1650-1653	NNP	denotes	DNA
T415	1654-1661	JJ	denotes	binding
T416	1662-1669	IN	denotes	through
T417	1670-1678	NNS	denotes	pathways
T418	1679-1683	WDT	denotes	that
T419	1684-1687	VBP	denotes	are
T420	1688-1697	RB	denotes	partially
T421	1698-1705	NNP	denotes	Protein
T422	1706-1712	NN	denotes	kinase
T423	1713-1726	NN	denotes	A-independent
T424	1726-1727	.	denotes	.
T425	1729-1739	NNP	denotes	CONCLUSION
T426	1740-1752	JJ	denotes	PTX-mediated
T427	1753-1770	NN	denotes	phosphodiesterase
T428	1771-1781	NN	denotes	inhibition
T429	1782-1788	VBZ	denotes	occurs
T430	1789-1798	RB	denotes	partially
T431	1799-1806	IN	denotes	through
T432	1807-1808	DT	denotes	a
T433	1809-1816	NNP	denotes	Protein
T434	1817-1823	NN	denotes	kinase
T435	1824-1837	JJ	denotes	A-independent
T436	1838-1845	NN	denotes	pathway
T437	1846-1849	CC	denotes	and
T438	1850-1853	MD	denotes	may
T439	1854-1859	VB	denotes	serve
T440	1860-1862	IN	denotes	as
T441	1863-1864	DT	denotes	a
T442	1865-1871	JJ	denotes	useful
T443	1872-1876	NN	denotes	tool
T444	1877-1879	IN	denotes	in
T445	1880-1883	DT	denotes	the
T446	1884-1895	NN	denotes	attenuation
T447	1896-1898	IN	denotes	of
T448	1899-1910	JJ	denotes	LPS-induced
T449	1911-1923	NN	denotes	inflammation
T450	1923-1924	.	denotes	.
T1162	1940-1943	DT	denotes	The
T1163	1944-1953	JJ	denotes	bacterial
T1164	1954-1962	NN	denotes	membrane
T1165	1963-1972	NN	denotes	component
T1166	1973-1991	NN	denotes	lipopolysaccharide
T1167	1992-1993	-LRB-	denotes	(
T1168	1993-1996	NNP	denotes	LPS
T1169	1996-1997	-RRB-	denotes	)
T1170	1998-2000	VBZ	denotes	is
T1171	2001-2008	JJ	denotes	capable
T1172	2009-2011	IN	denotes	of
T1173	2012-2022	VBG	denotes	initiating
T1174	2023-2038	NN	denotes	phosphorylation
T1175	2039-2042	CC	denotes	and
T1176	2043-2053	NN	denotes	activation
T1177	2054-2056	IN	denotes	of
T1178	2057-2065	JJ	denotes	multiple
T1179	2066-2070	NN	denotes	host
T1180	2071-2084	JJ	denotes	intracellular
T1181	2085-2092	NN	denotes	protein
T1182	2093-2100	NNS	denotes	kinases
T1183	2101-2104	CC	denotes	and
T1184	2105-2118	NN	denotes	transcription
T1185	2119-2126	NNS	denotes	factors
T1186	2126-2127	.	denotes	.
T1187	2128-2141	NNP	denotes	Transcription
T1188	2142-2148	NN	denotes	factor
T1189	2149-2159	NN	denotes	activation
T1190	2159-2160	,	denotes	,
T1191	2161-2166	WDT	denotes	which
T1192	2167-2174	VBZ	denotes	results
T1193	2175-2177	IN	denotes	in
T1194	2178-2181	DT	denotes	the
T1195	2182-2192	NN	denotes	modulation
T1196	2193-2195	IN	denotes	of
T1197	2196-2200	NN	denotes	gene
T1198	2201-2214	NN	denotes	transcription
T1199	2215-2218	CC	denotes	and
T1200	2219-2226	NN	denotes	protein
T1201	2227-2236	NN	denotes	synthesis
T1202	2236-2237	,	denotes	,
T1203	2238-2240	VBZ	denotes	is
T1204	2241-2242	DT	denotes	a
T1205	2243-2251	JJ	denotes	critical
T1206	2252-2259	NN	denotes	element
T1207	2260-2262	IN	denotes	in
T1208	2263-2266	DT	denotes	the
T1209	2267-2274	NN	denotes	defense
T1210	2275-2284	NN	denotes	mechanism
T1211	2285-2287	IN	denotes	of
T1212	2288-2291	DT	denotes	the
T1213	2292-2296	NN	denotes	host
T1214	2297-2303	JJ	denotes	immune
T1215	2304-2310	NN	denotes	system
T1216	2310-2314	CD	denotes	.1,2
T1217	2315-2326	JJ	denotes	LPS-induced
T1218	2327-2340	NN	denotes	transcription
T1219	2341-2347	NN	denotes	factor
T1220	2348-2358	NN	denotes	activation
T1221	2359-2362	VBZ	denotes	has
T1222	2363-2367	VBN	denotes	been
T1223	2368-2373	VBN	denotes	shown
T1224	2374-2376	TO	denotes	to
T1225	2377-2379	VB	denotes	be
T1226	2380-2381	DT	denotes	a
T1227	2382-2385	JJ	denotes	key
T1228	2386-2395	NN	denotes	regulator
T1229	2396-2398	IN	denotes	of
T1230	2399-2404	NN	denotes	tumor
T1231	2405-2413	NN	denotes	necrosis
T1232	2414-2422	NN	denotes	factor-α
T1233	2423-2424	-LRB-	denotes	(
T1234	2424-2429	NNP	denotes	TNF-α
T1235	2429-2430	-RRB-	denotes	)
T1236	2431-2441	NN	denotes	production
T1237	2441-2443	CD	denotes	.3
T1238	2444-2449	NNP	denotes	TNF-α
T1239	2450-2452	VBZ	denotes	is
T1240	2453-2454	DT	denotes	a
T1241	2455-2461	JJ	denotes	potent
T1242	2462-2478	JJ	denotes	pro-inflammatory
T1243	2479-2487	NN	denotes	cytokine
T1244	2488-2496	VBN	denotes	involved
T1245	2497-2499	IN	denotes	in
T1246	2500-2501	DT	denotes	a
T1247	2502-2506	JJ	denotes	wide
T1248	2507-2515	NN	denotes	spectrum
T1249	2516-2518	IN	denotes	of
T1250	2519-2527	JJ	denotes	cellular
T1251	2528-2537	NNS	denotes	responses
T1252	2537-2538	.	denotes	.
T1253	2539-2550	RB	denotes	Furthermore
T1254	2550-2551	,	denotes	,
T1255	2552-2557	JJ	denotes	TNF-α
T1256	2558-2567	NN	denotes	synthesis
T1257	2568-2571	MD	denotes	can
T1258	2572-2574	VB	denotes	be
T1259	2575-2585	VBN	denotes	attenuated
T1260	2586-2588	IN	denotes	in
T1261	2589-2595	JJ	denotes	immune
T1262	2596-2601	NNS	denotes	cells
T1263	2602-2609	VBN	denotes	exposed
T1264	2610-2612	TO	denotes	to
T1265	2613-2630	VB	denotes	phosphodiesterase
T1266	2631-2632	-LRB-	denotes	(
T1267	2632-2635	NNP	denotes	PDE
T1268	2635-2636	-RRB-	denotes	)
T1269	2637-2647	NN	denotes	inhibition
T1270	2648-2653	IN	denotes	after
T1271	2654-2663	NN	denotes	challenge
T1272	2664-2666	IN	denotes	by
T1273	2667-2668	DT	denotes	a
T1274	2669-2676	NN	denotes	variety
T1275	2677-2679	IN	denotes	of
T1276	2680-2696	JJ	denotes	pro-inflammatory
T1277	2697-2707	NNS	denotes	stimulants
T1278	2707-2709	CD	denotes	.4
T1279	2710-2713	DT	denotes	The
T1280	2714-2723	VBG	denotes	signaling
T1281	2724-2734	NNS	denotes	mechanisms
T1282	2735-2743	VBN	denotes	affected
T1283	2744-2746	IN	denotes	by
T1284	2747-2750	NNP	denotes	PDE
T1285	2751-2761	NN	denotes	inhibition
T1286	2761-2762	,	denotes	,
T1287	2763-2768	WDT	denotes	which
T1288	2769-2779	RB	denotes	ultimately
T1289	2780-2784	VBP	denotes	lead
T1290	2785-2787	TO	denotes	to
T1291	2788-2791	DT	denotes	the
T1292	2792-2806	NN	denotes	downregulation
T1293	2807-2809	IN	denotes	of
T1294	2810-2815	JJ	denotes	TNF-α
T1295	2816-2826	NN	denotes	production
T1296	2826-2827	,	denotes	,
T1297	2828-2832	VBP	denotes	have
T1298	2833-2836	RB	denotes	not
T1299	2837-2841	VBN	denotes	been
T1300	2842-2846	RB	denotes	well
T1301	2847-2860	VBN	denotes	characterized
T1302	2861-2863	IN	denotes	in
T1303	2864-2876	JJ	denotes	inflammatory
T1304	2877-2882	NNS	denotes	cells
T1305	2882-2883	.	denotes	.
T1306	2884-2895	NNP	denotes	Classically
T1307	2895-2896	,	denotes	,
T1308	2897-2899	PRP	denotes	it
T1309	2900-2903	VBZ	denotes	has
T1310	2904-2908	VBN	denotes	been
T1311	2909-2921	VBN	denotes	demonstrated
T1312	2922-2926	IN	denotes	that
T1313	2927-2930	NNP	denotes	PDE
T1314	2931-2941	NN	denotes	inhibition
T1315	2942-2949	NNS	denotes	results
T1316	2950-2952	IN	denotes	in
T1317	2953-2956	DT	denotes	the
T1318	2957-2970	JJ	denotes	intracellular
T1319	2971-2983	NN	denotes	accumulation
T1320	2984-2986	IN	denotes	of
T1321	2987-2990	DT	denotes	the
T1322	2991-2997	JJ	denotes	second
T1323	2998-3007	NN	denotes	messenger
T1324	3008-3014	JJ	denotes	cyclic
T1325	3015-3026	JJ	denotes	adenosine-3
T1326	3026-3028	SYM	denotes	,5
T1327	3028-3029	:	denotes	-
T1328	3029-3042	NN	denotes	monophosphate
T1329	3043-3044	-LRB-	denotes	(
T1330	3044-3048	NNP	denotes	cAMP
T1331	3048-3049	-RRB-	denotes	)
T1332	3050-3053	CC	denotes	and
T1333	3054-3064	JJ	denotes	subsequent
T1334	3065-3075	NN	denotes	activation
T1335	3076-3078	IN	denotes	of
T1336	3079-3086	NNP	denotes	Protein
T1337	3087-3093	VBP	denotes	kinase
T1338	3094-3095	DT	denotes	A
T1339	3096-3097	-LRB-	denotes	(
T1340	3097-3100	NNP	denotes	PKA
T1341	3100-3101	-RRB-	denotes	)
T1342	3101-3103	CD	denotes	.5
T1343	3104-3107	NNP	denotes	PKA
T1344	3108-3118	NN	denotes	activation
T1345	3119-3123	RB	denotes	then
T1346	3124-3129	VBZ	denotes	leads
T1347	3130-3132	TO	denotes	to
T1348	3133-3136	DT	denotes	the
T1349	3137-3152	NN	denotes	phosphorylation
T1350	3153-3155	IN	denotes	of
T1351	3156-3159	DT	denotes	the
T1352	3160-3173	NN	denotes	transcription
T1353	3174-3180	NN	denotes	factor
T1354	3181-3194	JJ	denotes	cAMP-response
T1355	3195-3202	NN	denotes	element
T1356	3203-3210	JJ	denotes	binding
T1357	3211-3218	NN	denotes	protein
T1358	3219-3220	-LRB-	denotes	(
T1359	3220-3224	NNP	denotes	CREB
T1360	3224-3225	-RRB-	denotes	)
T1361	3225-3226	,	denotes	,
T1362	3227-3239	NN	denotes	transmission
T1363	3240-3242	IN	denotes	of
T1364	3243-3250	NNS	denotes	signals
T1365	3251-3255	IN	denotes	into
T1366	3256-3259	DT	denotes	the
T1367	3260-3267	NN	denotes	nucleus
T1368	3267-3268	,	denotes	,
T1369	3269-3272	CC	denotes	and
T1370	3273-3276	DT	denotes	the
T1371	3277-3287	JJ	denotes	subsequent
T1372	3288-3298	NN	denotes	modulation
T1373	3299-3301	IN	denotes	of
T1374	3302-3306	NN	denotes	gene
T1375	3307-3320	NN	denotes	transcription
T1376	3320-3322	CD	denotes	.6
T1377	3323-3327	DT	denotes	This
T1378	3328-3338	RB	denotes	apparently
T1379	3339-3345	JJ	denotes	simple
T1380	3346-3352	JJ	denotes	linear
T1381	3353-3360	NN	denotes	cascade
T1382	3361-3365	VBZ	denotes	does
T1383	3366-3369	RB	denotes	not
T1384	3370-3375	RB	denotes	fully
T1385	3376-3383	VB	denotes	explain
T1386	3384-3387	DT	denotes	the
T1387	3388-3397	NN	denotes	mechanism
T1388	3398-3400	IN	denotes	by
T1389	3401-3406	WDT	denotes	which
T1390	3407-3409	DT	denotes	an
T1391	3410-3419	NN	denotes	elevation
T1392	3420-3422	IN	denotes	in
T1393	3423-3426	DT	denotes	the
T1394	3427-3440	JJ	denotes	intracellular
T1395	3441-3445	NNP	denotes	cAMP
T1396	3446-3451	NN	denotes	level
T1397	3452-3458	VBZ	denotes	exerts
T1398	3459-3471	JJ	denotes	wide-ranging
T1399	3472-3479	NNS	denotes	effects
T1400	3480-3482	IN	denotes	on
T1401	3483-3491	JJ	denotes	multiple
T1402	3492-3500	JJ	denotes	cellular
T1403	3501-3510	NNS	denotes	functions
T1404	3510-3511	.	denotes	.
T1405	3512-3517	EX	denotes	There
T1406	3518-3520	VBZ	denotes	is
T1407	3521-3522	DT	denotes	a
T1408	3523-3530	VBG	denotes	growing
T1409	3531-3535	NN	denotes	body
T1410	3536-3538	IN	denotes	of
T1411	3539-3547	NN	denotes	evidence
T1412	3548-3558	VBG	denotes	suggesting
T1413	3559-3563	IN	denotes	that
T1414	3564-3568	NNP	denotes	cAMP
T1415	3569-3572	MD	denotes	may
T1416	3573-3581	VB	denotes	function
T1417	3582-3589	IN	denotes	through
T1418	3590-3594	DT	denotes	both
T1419	3595-3608	JJ	denotes	PKA-dependent
T1420	3609-3612	CC	denotes	and
T1421	3613-3614	:	denotes	-
T1422	3614-3625	JJ	denotes	independent
T1423	3626-3636	NNS	denotes	mechanisms
T1424	3636-3638	CD	denotes	.6
T1425	3639-3640	CD	denotes	8
T1426	3641-3652	JJ	denotes	LPS-induced
T1427	3653-3663	NN	denotes	activation
T1428	3664-3666	IN	denotes	of
T1429	3667-3670	DT	denotes	the
T1430	3671-3684	NN	denotes	transcription
T1431	3685-3691	NN	denotes	factor
T1432	3692-3699	JJ	denotes	nuclear
T1433	3700-3709	NNP	denotes	factor-κB
T1434	3710-3711	-LRB-	denotes	(
T1435	3711-3716	NNP	denotes	NF-κB
T1436	3716-3717	-RRB-	denotes	)
T1437	3718-3721	VBZ	denotes	has
T1438	3722-3726	RB	denotes	also
T1439	3727-3731	VBN	denotes	been
T1440	3732-3735	DT	denotes	the
T1441	3736-3741	NN	denotes	focus
T1442	3742-3744	IN	denotes	of
T1443	3745-3746	DT	denotes	a
T1444	3747-3752	JJ	denotes	great
T1445	3753-3757	NN	denotes	deal
T1446	3758-3760	IN	denotes	of
T1447	3761-3769	NN	denotes	research
T1448	3769-3770	.	denotes	.
T1449	3771-3773	PRP	denotes	It
T1450	3774-3777	VBZ	denotes	has
T1451	3778-3782	VBN	denotes	been
T1452	3783-3790	RB	denotes	clearly
T1453	3791-3803	VBN	denotes	demonstrated
T1454	3804-3808	IN	denotes	that
T1455	3809-3815	NNS	denotes	agents
T1456	3816-3820	WDT	denotes	that
T1457	3821-3829	VBP	denotes	increase
T1458	3830-3843	JJ	denotes	intracellular
T1459	3844-3848	NNP	denotes	cAMP
T1460	3849-3853	RB	denotes	also
T1461	3854-3861	VBP	denotes	inhibit
T1462	3862-3877	JJ	denotes	NF-κB-dependent
T1463	3878-3894	JJ	denotes	pro-inflammatory
T1464	3895-3899	NN	denotes	gene
T1465	3900-3913	NN	denotes	transcription
T1466	3913-3914	,	denotes	,
T1467	3915-3927	RB	denotes	particularly
T1468	3928-3930	IN	denotes	of
T1469	3931-3934	DT	denotes	the
T1470	3935-3940	NNP	denotes	TNF-α
T1471	3941-3945	NN	denotes	gene
T1472	3945-3947	CD	denotes	.9
T1473	3948-3959	NNP	denotes	Controversy
T1474	3960-3966	VBZ	denotes	exists
T1475	3967-3976	VBG	denotes	regarding
T1476	3977-3980	DT	denotes	the
T1477	3981-3986	JJ	denotes	exact
T1478	3987-3996	NN	denotes	mechanism
T1479	3996-3997	-LRB-	denotes	(
T1480	3997-3998	PRP	denotes	s
T1481	3998-3999	-RRB-	denotes	)
T1482	4000-4002	IN	denotes	by
T1483	4003-4008	WDT	denotes	which
T1484	4009-4012	NNP	denotes	PDE
T1485	4013-4023	NN	denotes	inhibition
T1486	4024-4038	VBZ	denotes	down-regulates
T1487	4039-4044	JJ	denotes	TNF-α
T1488	4045-4055	NN	denotes	production
T1489	4055-4056	.	denotes	.
T1490	4057-4070	NNS	denotes	Possibilities
T1491	4071-4078	VBP	denotes	include
T1492	4078-4079	,	denotes	,
T1493	4080-4083	CC	denotes	but
T1494	4084-4087	VBP	denotes	are
T1495	4088-4091	RB	denotes	not
T1496	4092-4099	VBN	denotes	limited
T1497	4100-4102	TO	denotes	to
T1498	4102-4103	,	denotes	,
T1499	4104-4114	NN	denotes	inhibition
T1500	4115-4117	IN	denotes	of
T1501	4118-4123	NNP	denotes	NF-κB
T1502	4124-4127	NNP	denotes	DNA
T1503	4128-4135	JJ	denotes	binding
T1504	4136-4144	NN	denotes	activity
T1505	4144-4145	,	denotes	,
T1506	4146-4160	NN	denotes	downregulation
T1507	4161-4163	IN	denotes	of
T1508	4164-4169	JJ	denotes	NF-κB
T1509	4170-4185	JJ	denotes	transcriptional
T1510	4186-4194	NN	denotes	activity
T1511	4194-4195	,	denotes	,
T1512	4196-4205	VBD	denotes	increased
T1513	4206-4210	NNP	denotes	CREB
T1514	4211-4221	NN	denotes	activation
T1515	4221-4222	,	denotes	,
T1516	4223-4226	CC	denotes	and
T1517	4227-4238	NN	denotes	competition
T1518	4239-4246	IN	denotes	between
T1519	4247-4252	NNP	denotes	NF-κB
T1520	4253-4256	CC	denotes	and
T1521	4257-4261	NNP	denotes	CREB
T1522	4262-4265	IN	denotes	for
T1523	4266-4272	JJ	denotes	common
T1524	4273-4286	NNS	denotes	co-activators
T1525	4287-4291	JJ	denotes	such
T1526	4292-4294	IN	denotes	as
T1527	4295-4299	NNP	denotes	CREB
T1528	4300-4307	JJ	denotes	binding
T1529	4308-4315	NN	denotes	protein
T1530	4316-4317	-LRB-	denotes	(
T1531	4317-4320	NNP	denotes	CBP
T1532	4320-4321	-RRB-	denotes	)
T1533	4321-4322	.	denotes	.
T1534	4323-4325	PRP	denotes	It
T1535	4326-4328	VBZ	denotes	is
T1536	4329-4333	RB	denotes	also
T1537	4334-4337	RB	denotes	not
T1538	4338-4343	VBN	denotes	known
T1539	4344-4351	IN	denotes	whether
T1540	4352-4357	DT	denotes	these
T1541	4358-4367	NNS	denotes	processes
T1542	4368-4372	VBP	denotes	rely
T1543	4373-4379	RB	denotes	solely
T1544	4380-4384	IN	denotes	upon
T1545	4385-4388	DT	denotes	the
T1546	4389-4399	NN	denotes	activation
T1547	4400-4402	IN	denotes	of
T1548	4403-4406	NNP	denotes	PKA
T1549	4406-4407	.	denotes	.
T1550	4408-4417	RB	denotes	Therefore
T1551	4417-4418	,	denotes	,
T1552	4419-4422	DT	denotes	the
T1553	4423-4432	NN	denotes	objective
T1554	4433-4435	IN	denotes	of
T1555	4436-4439	DT	denotes	the
T1556	4440-4447	JJ	denotes	present
T1557	4448-4453	NN	denotes	study
T1558	4454-4456	VBZ	denotes	is
T1559	4457-4459	TO	denotes	to
T1560	4460-4469	VB	denotes	determine
T1561	4470-4473	DT	denotes	the
T1562	4474-4481	NNS	denotes	effects
T1563	4482-4484	IN	denotes	of
T1564	4485-4496	JJ	denotes	nonspecific
T1565	4497-4500	NNP	denotes	PDE
T1566	4501-4511	NN	denotes	inhibition
T1567	4512-4516	IN	denotes	with
T1568	4517-4518	LS	denotes	1
T1569	4518-4519	:	denotes	-
T1570	4519-4520	NNP	denotes	[
T1571	4520-4530	JJ	denotes	5-oxohexyl
T1572	4530-4531	NNP	denotes	]
T1573	4531-4535	CD	denotes	-3,7
T1574	4535-4536	:	denotes	-
T1575	4536-4552	NN	denotes	dimethylxanthine
T1576	4553-4554	-LRB-	denotes	(
T1577	4554-4568	NNP	denotes	Pentoxifylline
T1578	4568-4569	:	denotes	;
T1579	4570-4573	NNP	denotes	PTX
T1580	4573-4574	-RRB-	denotes	)
T1581	4575-4577	IN	denotes	on
T1582	4578-4583	NN	denotes	NF-κB
T1583	4584-4587	CC	denotes	and
T1584	4588-4592	NNP	denotes	CREB
T1585	4593-4603	NN	denotes	activation
T1586	4604-4606	IN	denotes	in
T1587	4607-4612	NN	denotes	vitro
T1588	4613-4615	IN	denotes	in
T1589	4616-4621	JJ	denotes	human
T1590	4622-4633	NN	denotes	mononuclear
T1591	4634-4639	NNS	denotes	cells
T1592	4639-4640	.	denotes	.
T1593	4641-4645	IN	denotes	With
T1594	4646-4649	DT	denotes	the
T1595	4650-4653	NN	denotes	use
T1596	4654-4656	IN	denotes	of
T1597	4657-4665	JJ	denotes	specific
T1598	4666-4676	NNS	denotes	inhibitors
T1599	4676-4677	,	denotes	,
T1600	4678-4680	PRP	denotes	we
T1601	4681-4685	RB	denotes	also
T1602	4686-4698	VBD	denotes	investigated
T1603	4699-4702	DT	denotes	the
T1604	4703-4707	NN	denotes	role
T1605	4708-4710	IN	denotes	of
T1606	4711-4714	NNP	denotes	PKA
T1607	4715-4717	IN	denotes	in
T1608	4718-4729	JJ	denotes	LPS-induced
T1609	4730-4735	JJ	denotes	TNF-α
T1610	4736-4746	NN	denotes	production
T1611	4746-4747	.	denotes	.
T2464	0-4	DT	denotes	this
T2465	5-10	NN	denotes	study
T2466	11-14	VBD	denotes	was
T2467	4786-4794	VBN	denotes	approved
T2468	4795-4797	IN	denotes	by
T2469	4798-4801	DT	denotes	the
T2470	4802-4807	NNP	denotes	Human
T2471	4808-4816	NNP	denotes	Research
T2472	4817-4828	NNPS	denotes	Protections
T2473	4829-4836	NNP	denotes	Program
T2474	4837-4840	CC	denotes	and
T2475	4841-4844	DT	denotes	the
T2476	4845-4858	JJ	denotes	Institutional
T2477	4859-4865	NNP	denotes	Review
T2478	4866-4871	NNP	denotes	Board
T2479	4871-4872	.	denotes	.
T2480	4873-4880	VBN	denotes	Written
T2481	4881-4888	NN	denotes	consent
T2482	4889-4891	TO	denotes	to
T2483	4892-4903	VB	denotes	participate
T2484	4904-4906	IN	denotes	in
T2485	4907-4910	DT	denotes	the
T2486	4911-4916	NN	denotes	study
T2487	4917-4920	VBD	denotes	was
T2488	4921-4929	VBN	denotes	obtained
T2489	4930-4934	IN	denotes	from
T2490	4935-4938	DT	denotes	all
T2491	4939-4949	NNS	denotes	volunteers
T2492	4950-4955	RB	denotes	prior
T2493	4956-4958	TO	denotes	to
T2494	4959-4964	NN	denotes	blood
T2495	4965-4986	VBN	denotes	donation. Heparinized
T2496	4987-4998	NNS	denotes	Vacutainers
T2497	4999-5003	VBD	denotes	were
T2498	5004-5013	VBN	denotes	purchased
T2499	5014-5018	IN	denotes	from
T2500	5019-5025	NNP	denotes	Becton
T2501	5026-5035	NNP	denotes	Dickinson
T2502	5036-5037	-LRB-	denotes	(
T2503	5037-5040	NNP	denotes	San
T2504	5041-5045	NNP	denotes	Jose
T2505	5045-5046	,	denotes	,
T2506	5047-5049	NNP	denotes	CA
T2507	5049-5050	-RRB-	denotes	)
T2508	5050-5051	.	denotes	.
T152	0-4	DT	denotes	this
T153	5-10	NN	denotes	study
T154	11-14	VBD	denotes	was
T155	15-17	TO	denotes	to
T156	18-27	VB	denotes	determine
T157	28-31	DT	denotes	the
T158	32-38	NN	denotes	effect
T159	39-41	IN	denotes	of
T160	42-53	JJ	denotes	nonspecific
T161	54-71	NN	denotes	phosphodiesterase
R78	T180	T162	punct	-,inhibition
R79	T181	T154	conj	stimulated,was
R80	T182	T184	amod	human,cells
R81	T183	T184	compound	mononuclear,cells
R82	T184	T181	dobj	cells,stimulated
R83	T185	T154	punct	.,was
R84	T186	T194	advmod	INTRODUCTION,is
R85	T187	T188	det	The,production
R86	T188	T194	nsubj	production,is
R87	T189	T188	prep	of,production
R88	T190	T189	pobj	TNF-α,of
R89	T191	T188	prep	following,production
R90	T192	T193	compound	LPS,stimulation
R91	T193	T191	pobj	stimulation,following
R92	T194	T194	ROOT	is,is
R93	T195	T194	attr	one,is
R94	T196	T195	prep	of,one
R95	T197	T199	det	the,steps
R96	T198	T199	amod	key,steps
R97	T199	T196	pobj	steps,of
R98	T200	T199	prep	in,steps
R99	T201	T202	amod	bacterial,sepsis
R103	T205	T194	punct	.,is
R104	T206	T207	det	The,mechanism
R105	T207	T220	nsubj	mechanism,remains
R106	T208	T212	prep	by,alters
R107	T209	T208	pobj	which,by
R108	T210	T211	amod	phosphodiesterase,inhibition
R109	T211	T212	nsubj	inhibition,alters
R110	T212	T207	relcl	alters,mechanism
R111	T213	T214	amod	TNF-α,production
R112	T214	T212	dobj	production,alters
R113	T215	T212	prep	in,alters
R114	T216	T217	det	the,presence
R115	T217	T215	pobj	presence,in
R116	T218	T217	prep	of,presence
R117	T219	T218	pobj	LPS,of
R118	T220	T220	ROOT	remains,remains
R119	T221	T220	acomp	unclear,remains
R120	T222	T220	punct	.,remains
R121	T223	T226	compound	METHODS,cells
R122	T224	T226	compound	Human,cells
R123	T225	T226	compound	mononuclear,cells
R124	T226	T228	nsubjpass	cells,stimulated
R125	T227	T228	auxpass	were,stimulated
R126	T228	T249	ccomp	stimulated,mM
R127	T229	T228	prep	with,stimulated
R128	T230	T229	pobj	LPS,with
R129	T231	T235	punct	(,mL
R130	T232	T233	nummod	1,μg
R131	T233	T235	compound	μg,mL
R132	T234	T235	compound	/,mL
R133	T235	T230	appos	mL,LPS
R134	T236	T235	punct	),mL
R135	T237	T230	punct	",",LPS
R136	T238	T228	prep	in,stimulated
R137	T239	T240	det	the,presence
R138	T240	T238	pobj	presence,in
R139	T241	T240	cc	and,presence
R140	T242	T240	conj	absence,presence
R141	T243	T242	prep	of,absence
R142	T244	T243	pobj	Pentoxifylline,of
R143	T245	T246	punct	(,PTX
R144	T246	T244	appos	PTX,Pentoxifylline
R145	T247	T249	punct	;,mM
R146	T248	T249	nummod	20,mM
R147	T249	T249	ROOT	mM,mM
R148	T250	T249	punct	),mM
R149	T251	T249	punct	",",mM
R150	T252	T255	det	a,inhibitor
R151	T253	T255	amod	nonspecific,inhibitor
R152	T254	T255	compound	phosphodiesterase,inhibitor
R153	T255	T249	appos	inhibitor,mM
R154	T256	T249	punct	.,mM
R155	T257	T258	amod	Western,blotting
R156	T258	T281	nsubjpass	blotting,performed
R157	T259	T258	prep	of,blotting
R158	T260	T262	amod	phosphorylated,I-κBα
R159	T261	T262	compound	cytoplasmic,I-κBα
R160	T262	T259	pobj	I-κBα,of
R161	T263	T262	punct	",",I-κBα
R162	T264	T266	amod	nuclear,p65
R163	T265	T266	compound	factor-κB,p65
R164	T266	T262	appos	p65,I-κBα
R165	T267	T266	punct	(,p65
R166	T268	T266	appos	NF-κB,p65
R167	T269	T266	punct	),p65
R168	T270	T266	punct	",",p65
R169	T271	T266	cc	and,p65
R170	T272	T274	amod	nuclear,element
R171	T273	T274	amod	cAMP-response,element
R172	T274	T276	compound	element,protein
R173	T275	T276	amod	binding,protein
R174	T276	T266	conj	protein,p65
R175	T277	T278	punct	(,CREB
R176	T278	T276	appos	CREB,protein
R177	T279	T276	punct	),protein
R178	T280	T281	auxpass	was,performed
R179	T281	T281	ROOT	performed,performed
R180	T282	T281	punct	.,performed
R181	T283	T284	npadvmod	DNA,binding
R182	T284	T290	nsubjpass	binding,verified
R183	T285	T284	prep	of,binding
R184	T286	T285	pobj	NF-κB,of
R185	T287	T286	cc	and,NF-κB
R186	T288	T286	conj	CREB,NF-κB
R187	T289	T290	auxpass	was,verified
R188	T290	T290	ROOT	verified,verified
R189	T291	T290	agent	by,verified
R190	T292	T295	amod	electrophoretic,assay
R191	T293	T294	compound	mobility,shift
R192	T294	T295	compound	shift,assay
R193	T295	T291	pobj	assay,by
R194	T296	T290	punct	.,verified
R195	T297	T298	amod	TNF-α,levels
R196	T298	T300	nsubjpass	levels,determined
R197	T299	T300	auxpass	were,determined
R198	T300	T300	ROOT	determined,determined
R199	T301	T300	prep	in,determined
R200	T302	T303	det	the,supernatant
R201	T303	T301	pobj	supernatant,in
R202	T304	T303	prep	of,supernatant
R203	T305	T306	amod	stimulated,cells
R204	T306	T304	pobj	cells,of
R205	T307	T303	prep	in,supernatant
R206	T308	T309	det	the,presence
R207	T309	T307	pobj	presence,in
R208	T310	T309	cc	and,presence
R209	T311	T309	conj	absence,presence
R210	T312	T313	compound	Protein,kinase
R211	T313	T313	ROOT	kinase,kinase
R212	T314	T315	det	A,inhibition
R213	T315	T315	ROOT	inhibition,inhibition
R214	T316	T315	prep	by,inhibition
R215	T317	T320	det	an,assay
R216	T318	T320	nmod	enzyme-linked,assay
R217	T319	T320	compound	immunosorbent,assay
R218	T320	T316	pobj	assay,by
R219	T321	T320	punct	(,assay
R220	T322	T320	appos	ELISA,assay
R221	T323	T320	punct	),assay
R222	T324	T315	punct	.,inhibition
R223	T325	T326	compound	RESULTS,PTX
R224	T326	T328	nsubjpass	PTX,demonstrated
R225	T327	T328	auxpass	was,demonstrated
R226	T328	T328	ROOT	demonstrated,demonstrated
R227	T329	T331	aux	to,reduce
R228	T330	T331	advmod	significantly,reduce
R229	T331	T328	xcomp	reduce,demonstrated
R230	T332	T334	amod	cytoplasmic,phosphorylation
R231	T333	T334	amod	I-κBα,phosphorylation
R232	T334	T331	dobj	phosphorylation,reduce
R233	T335	T334	punct	",",phosphorylation
R234	T336	T337	amod	nuclear,p65
R235	T337	T338	compound	p65,phosphorylation
R236	T338	T334	appos	phosphorylation,phosphorylation
R237	T339	T338	punct	",",phosphorylation
R238	T340	T338	cc	and,phosphorylation
R239	T341	T344	det	the,activity
R240	T342	T344	nmod	DNA,activity
R241	T343	T344	amod	binding,activity
R242	T344	T338	conj	activity,phosphorylation
R243	T345	T344	prep	of,activity
R244	T346	T345	pobj	NF-κB,of
R245	T347	T328	punct	.,demonstrated
R246	T348	T353	prep	In,enhanced
R247	T349	T348	pobj	contrast,In
R248	T350	T353	punct	",",enhanced
R249	T351	T353	nsubj	PTX,enhanced
R250	T352	T353	advmod	markedly,enhanced
R251	T353	T353	ROOT	enhanced,enhanced
R252	T354	T355	det	the,phosphorylation
R253	T355	T353	dobj	phosphorylation,enhanced
R254	T356	T355	cc	and,phosphorylation
R255	T357	T359	amod	DNA,activity
R256	T358	T359	amod	binding,activity
R257	T359	T355	conj	activity,phosphorylation
R258	T360	T355	prep	of,phosphorylation
R259	T361	T360	pobj	CREB,of
R260	T362	T353	punct	.,enhanced
R261	T363	T370	nsubj	Cells,secreted
R262	T364	T365	advmod	concomitantly,treated
R263	T365	T363	acl	treated,Cells
R264	T366	T365	prep	with,treated
R265	T367	T366	pobj	PTX,with
R266	T368	T367	cc	and,PTX
R267	T369	T367	conj	LPS,PTX
R268	T370	T370	ROOT	secreted,secreted
R269	T371	T372	amod	similar,levels
R270	T372	T370	dobj	levels,secreted
R271	T373	T372	prep	of,levels
R272	T374	T373	pobj	TNF-α,of
R273	T375	T370	prep	in,secreted
R274	T376	T377	det	the,presence
R275	T377	T375	pobj	presence,in
R276	T378	T377	cc	and,presence
R277	T379	T377	conj	absence,presence
R278	T380	T381	compound	Protein,kinase
R279	T381	T381	ROOT	kinase,kinase
R280	T382	T383	det	A,inhibition
R281	T383	T383	ROOT	inhibition,inhibition
R282	T384	T383	punct	.,inhibition
R283	T385	T396	nsubj	DISCUSSION,affects
R284	T386	T388	det	The,level
R285	T387	T388	amod	increased,level
R286	T388	T396	nsubj	level,affects
R287	T389	T388	prep	of,level
R288	T390	T389	pobj	cAMP,of
R289	T391	T392	nsubj	that,results
R290	T392	T388	relcl	results,level
R291	T393	T392	prep	from,results
R292	T394	T395	amod	phosphodiesterase,inhibition
R293	T395	T393	pobj	inhibition,from
R294	T396	T396	ROOT	affects,affects
R295	T397	T400	amod	cytoplasmic,events
R296	T398	T397	cc	and,cytoplasmic
R297	T399	T397	conj	nuclear,cytoplasmic
R298	T400	T396	dobj	events,affects
R299	T401	T396	punct	",",affects
R300	T402	T396	advcl	resulting,affects
R301	T403	T402	prep	in,resulting
R302	T404	T405	det	the,attenuation
R303	T405	T403	pobj	attenuation,in
R304	T406	T405	prep	of,attenuation
R305	T407	T406	pobj	NF-κB,of
R306	T408	T405	cc	and,attenuation
R307	T409	T410	det	the,activation
R308	T410	T405	conj	activation,attenuation
R309	T411	T410	prep	of,activation
R310	T412	T414	compound	CREB,DNA
R311	T413	T414	compound	transcriptional,DNA
R312	T414	T411	pobj	DNA,of
R313	T415	T410	amod	binding,activation
R314	T416	T415	prep	through,binding
R315	T417	T416	pobj	pathways,through
R316	T418	T419	nsubj	that,are
R317	T419	T417	relcl	are,pathways
R318	T420	T419	advmod	partially,are
R319	T421	T423	compound	Protein,A-independent
R320	T422	T423	compound	kinase,A-independent
R321	T423	T419	attr	A-independent,are
R322	T424	T396	punct	.,affects
R323	T425	T428	nmod	CONCLUSION,inhibition
R324	T426	T428	amod	PTX-mediated,inhibition
R325	T427	T428	compound	phosphodiesterase,inhibition
R326	T428	T429	nsubj	inhibition,occurs
R327	T429	T429	ROOT	occurs,occurs
R328	T430	T431	advmod	partially,through
R329	T431	T429	prep	through,occurs
R330	T432	T436	det	a,pathway
R331	T433	T436	nmod	Protein,pathway
R332	T434	T436	nmod	kinase,pathway
R333	T435	T436	compound	A-independent,pathway
R334	T436	T431	pobj	pathway,through
R335	T437	T429	cc	and,occurs
R336	T438	T439	aux	may,serve
R337	T439	T429	conj	serve,occurs
R338	T440	T439	prep	as,serve
R348	T450	T429	punct	.,occurs
R777	T152	T153	det	this,study
R779	T154	T154	ROOT	was,was
R780	T155	T156	aux	to,determine
R781	T156	T154	xcomp	determine,was
R782	T157	T158	det	the,effect
R783	T158	T156	dobj	effect,determine
R784	T159	T158	prep	of,effect
R785	T160	T162	amod	nonspecific,inhibition
R786	T161	T162	compound	phosphodiesterase,inhibition
R848	T1162	T1165	det	The,component
R849	T1163	T1165	amod	bacterial,component
R850	T1164	T1165	compound	membrane,component
R851	T1165	T1170	nsubj	component,is
R852	T1166	T1170	nsubj	lipopolysaccharide,is
R853	T1167	T1168	punct	(,LPS
R854	T1168	T1166	appos	LPS,lipopolysaccharide
R855	T1169	T1166	punct	),lipopolysaccharide
R856	T1170	T1170	ROOT	is,is
R857	T1171	T1170	acomp	capable,is
R858	T1172	T1171	prep	of,capable
R859	T1173	T1172	pcomp	initiating,of
R860	T1174	T1173	dobj	phosphorylation,initiating
R861	T1175	T1174	cc	and,phosphorylation
R862	T1176	T1174	conj	activation,phosphorylation
R863	T1177	T1174	prep	of,phosphorylation
R864	T1178	T1182	amod	multiple,kinases
R865	T1179	T1182	nmod	host,kinases
R866	T1180	T1182	amod	intracellular,kinases
R867	T1181	T1182	compound	protein,kinases
R868	T1182	T1177	pobj	kinases,of
R869	T1183	T1182	cc	and,kinases
R870	T1184	T1185	compound	transcription,factors
R871	T1185	T1182	conj	factors,kinases
R872	T1186	T1170	punct	.,is
R873	T1187	T1188	compound	Transcription,factor
R874	T1188	T1189	compound	factor,activation
R875	T1189	T1203	nsubj	activation,is
R876	T1190	T1189	punct	",",activation
R877	T1191	T1192	nsubj	which,results
R878	T1192	T1189	relcl	results,activation
R879	T1193	T1192	prep	in,results
R880	T1194	T1195	det	the,modulation
R881	T1195	T1193	pobj	modulation,in
R882	T1196	T1195	prep	of,modulation
R883	T1197	T1198	compound	gene,transcription
R884	T1198	T1196	pobj	transcription,of
R885	T1199	T1198	cc	and,transcription
R886	T1200	T1201	compound	protein,synthesis
R887	T1201	T1198	conj	synthesis,transcription
R888	T1202	T1203	punct	",",is
R889	T1203	T1203	ROOT	is,is
R890	T1204	T1206	det	a,element
R891	T1205	T1206	amod	critical,element
R892	T1206	T1203	attr	element,is
R893	T1207	T1206	prep	in,element
R894	T1208	T1210	det	the,mechanism
R895	T1209	T1210	compound	defense,mechanism
R896	T1210	T1207	pobj	mechanism,in
R897	T1211	T1210	prep	of,mechanism
R898	T1212	T1215	det	the,system
R899	T1213	T1215	nmod	host,system
R900	T1214	T1215	amod	immune,system
R901	T1215	T1211	pobj	system,of
R902	T1216	T1220	nummod	".1,2",activation
R903	T1217	T1220	amod	LPS-induced,activation
R904	T1218	T1219	compound	transcription,factor
R905	T1219	T1220	compound	factor,activation
R906	T1220	T1223	nsubjpass	activation,shown
R907	T1221	T1223	aux	has,shown
R908	T1222	T1223	auxpass	been,shown
R909	T1223	T1223	ROOT	shown,shown
R910	T1224	T1225	aux	to,be
R911	T1225	T1223	xcomp	be,shown
R912	T1226	T1228	det	a,regulator
R913	T1227	T1228	amod	key,regulator
R914	T1228	T1225	attr	regulator,be
R915	T1229	T1228	prep	of,regulator
R916	T1230	T1231	compound	tumor,necrosis
R917	T1231	T1232	compound	necrosis,factor-α
R918	T1232	T1229	pobj	factor-α,of
R919	T1233	T1234	punct	(,TNF-α
R920	T1234	T1232	appos	TNF-α,factor-α
R921	T1235	T1232	punct	),factor-α
R922	T1236	T1238	compound	production,TNF-α
R923	T1237	T1238	nummod	.3,TNF-α
R924	T1238	T1228	appos	TNF-α,regulator
R938	T1252	T1203	punct	.,is
R939	T1253	T1259	advmod	Furthermore,attenuated
R940	T1254	T1259	punct	",",attenuated
R941	T1255	T1256	amod	TNF-α,synthesis
R942	T1256	T1259	nsubjpass	synthesis,attenuated
R943	T1257	T1259	aux	can,attenuated
R944	T1258	T1259	auxpass	be,attenuated
R945	T1259	T1259	ROOT	attenuated,attenuated
R946	T1260	T1259	prep	in,attenuated
R947	T1261	T1262	amod	immune,cells
R948	T1262	T1260	pobj	cells,in
R949	T1263	T1259	conj	exposed,attenuated
R950	T1264	T1265	aux	to,phosphodiesterase
R951	T1265	T1263	advcl	phosphodiesterase,exposed
R952	T1266	T1267	punct	(,PDE
R953	T1267	T1265	appos	PDE,phosphodiesterase
R954	T1268	T1259	punct	),attenuated
R955	T1269	T1301	nsubjpass	inhibition,characterized
R956	T1270	T1269	prep	after,inhibition
R957	T1271	T1270	pobj	challenge,after
R958	T1272	T1271	prep	by,challenge
R959	T1273	T1274	det	a,variety
R960	T1274	T1272	pobj	variety,by
R961	T1275	T1274	prep	of,variety
R962	T1276	T1277	amod	pro-inflammatory,stimulants
R963	T1277	T1275	pobj	stimulants,of
R964	T1278	T1271	nummod	.4,challenge
R965	T1279	T1281	det	The,mechanisms
R966	T1280	T1281	amod	signaling,mechanisms
R967	T1281	T1301	nsubjpass	mechanisms,characterized
R968	T1282	T1281	acl	affected,mechanisms
R969	T1283	T1282	agent	by,affected
R970	T1284	T1285	compound	PDE,inhibition
R971	T1285	T1283	pobj	inhibition,by
R972	T1286	T1285	punct	",",inhibition
R973	T1287	T1289	nsubj	which,lead
R974	T1288	T1289	advmod	ultimately,lead
R975	T1289	T1285	relcl	lead,inhibition
R976	T1290	T1289	prep	to,lead
R977	T1291	T1292	det	the,downregulation
R978	T1292	T1290	pobj	downregulation,to
R979	T1293	T1292	prep	of,downregulation
R980	T1294	T1295	compound	TNF-α,production
R981	T1295	T1293	pobj	production,of
R982	T1296	T1301	punct	",",characterized
R983	T1297	T1301	aux	have,characterized
R984	T1298	T1301	neg	not,characterized
R985	T1299	T1301	auxpass	been,characterized
R986	T1300	T1301	advmod	well,characterized
R987	T1301	T1301	ROOT	characterized,characterized
R988	T1302	T1301	prep	in,characterized
R989	T1303	T1304	amod	inflammatory,cells
R990	T1304	T1302	pobj	cells,in
R991	T1305	T1301	punct	.,characterized
R992	T1306	T1311	advmod	Classically,demonstrated
R993	T1307	T1311	punct	",",demonstrated
R994	T1308	T1311	nsubjpass	it,demonstrated
R995	T1309	T1311	aux	has,demonstrated
R996	T1310	T1311	auxpass	been,demonstrated
R997	T1311	T1311	ROOT	demonstrated,demonstrated
R998	T1312	T1337	mark	that,kinase
R999	T1313	T1315	compound	PDE,results
R1000	T1314	T1315	compound	inhibition,results
R1001	T1315	T1337	nsubj	results,kinase
R1002	T1316	T1315	prep	in,results
R1003	T1317	T1319	det	the,accumulation
R1004	T1318	T1319	amod	intracellular,accumulation
R1005	T1319	T1316	pobj	accumulation,in
R1006	T1320	T1319	prep	of,accumulation
R1007	T1321	T1323	det	the,messenger
R1008	T1322	T1323	amod	second,messenger
R1009	T1323	T1320	pobj	messenger,of
R1010	T1324	T1328	nmod	cyclic,monophosphate
R1011	T1325	T1328	amod	adenosine-3,monophosphate
R1012	T1326	T1328	punct	",5",monophosphate
R1013	T1327	T1328	punct	-,monophosphate
R1014	T1328	T1315	appos	monophosphate,results
R1015	T1329	T1330	punct	(,cAMP
R1016	T1330	T1328	appos	cAMP,monophosphate
R1017	T1331	T1330	punct	),cAMP
R1018	T1332	T1328	cc	and,monophosphate
R1019	T1333	T1334	amod	subsequent,activation
R1020	T1334	T1328	conj	activation,monophosphate
R1021	T1335	T1334	prep	of,activation
R1022	T1336	T1335	pobj	Protein,of
R1023	T1337	T1311	ccomp	kinase,demonstrated
R1024	T1338	T1340	det	A,PKA
R1025	T1339	T1340	punct	(,PKA
R1026	T1340	T1337	appos	PKA,kinase
R1027	T1341	T1340	punct	),PKA
R1028	T1342	T1344	nummod	.5,activation
R1029	T1343	T1344	compound	PKA,activation
R1030	T1344	T1346	nsubj	activation,leads
R1031	T1345	T1346	advmod	then,leads
R1032	T1346	T1346	ROOT	leads,leads
R1033	T1347	T1346	prep	to,leads
R1034	T1348	T1349	det	the,phosphorylation
R1035	T1349	T1347	pobj	phosphorylation,to
R1036	T1350	T1349	prep	of,phosphorylation
R1037	T1351	T1353	det	the,factor
R1038	T1352	T1353	compound	transcription,factor
R1039	T1353	T1350	pobj	factor,of
R1040	T1354	T1355	amod	cAMP-response,element
R1041	T1355	T1365	npadvmod	element,into
R1042	T1356	T1357	amod	binding,protein
R1043	T1357	T1355	appos	protein,element
R1044	T1358	T1359	punct	(,CREB
R1045	T1359	T1357	appos	CREB,protein
R1046	T1360	T1359	punct	),CREB
R1047	T1361	T1357	punct	",",protein
R1048	T1362	T1357	conj	transmission,protein
R1049	T1363	T1362	prep	of,transmission
R1050	T1364	T1363	pobj	signals,of
R1051	T1365	T1349	prep	into,phosphorylation
R1052	T1366	T1367	det	the,nucleus
R1053	T1367	T1365	pobj	nucleus,into
R1054	T1368	T1346	punct	",",leads
R1055	T1369	T1346	cc	and,leads
R1056	T1370	T1372	det	the,modulation
R1057	T1371	T1372	amod	subsequent,modulation
R1058	T1372	T1346	conj	modulation,leads
R1059	T1373	T1372	prep	of,modulation
R1060	T1374	T1375	compound	gene,transcription
R1061	T1375	T1373	pobj	transcription,of
R1062	T1376	T1375	nummod	.6,transcription
R1063	T1377	T1381	det	This,cascade
R1064	T1378	T1379	advmod	apparently,simple
R1065	T1379	T1381	amod	simple,cascade
R1066	T1380	T1381	amod	linear,cascade
R1067	T1381	T1385	nsubj	cascade,explain
R1068	T1382	T1385	aux	does,explain
R1069	T1383	T1385	neg	not,explain
R1070	T1384	T1385	advmod	fully,explain
R1071	T1385	T1385	ROOT	explain,explain
R1072	T1386	T1387	det	the,mechanism
R1073	T1387	T1385	dobj	mechanism,explain
R1074	T1388	T1397	prep	by,exerts
R1075	T1389	T1388	pobj	which,by
R1076	T1390	T1391	det	an,elevation
R1077	T1391	T1397	nsubj	elevation,exerts
R1078	T1392	T1391	prep	in,elevation
R1079	T1393	T1396	det	the,level
R1080	T1394	T1396	amod	intracellular,level
R1081	T1395	T1396	compound	cAMP,level
R1082	T1396	T1392	pobj	level,in
R1083	T1397	T1387	relcl	exerts,mechanism
R1084	T1398	T1399	amod	wide-ranging,effects
R1085	T1399	T1397	dobj	effects,exerts
R1086	T1400	T1399	prep	on,effects
R1087	T1401	T1403	amod	multiple,functions
R1088	T1402	T1403	amod	cellular,functions
R1089	T1403	T1400	pobj	functions,on
R1090	T1404	T1385	punct	.,explain
R1091	T1405	T1406	expl	There,is
R1092	T1406	T1406	ROOT	is,is
R1093	T1407	T1409	det	a,body
R1094	T1408	T1409	amod	growing,body
R1100	T1414	T1416	nsubj	cAMP,function
R1101	T1415	T1416	aux	may,function
R1102	T1416	T1412	ccomp	function,suggesting
R1103	T1417	T1416	prep	through,function
R1104	T1418	T1419	preconj	both,PKA-dependent
R1105	T1419	T1423	amod	PKA-dependent,mechanisms
R1106	T1420	T1419	cc	and,PKA-dependent
R1107	T1421	T1419	punct	-,PKA-dependent
R1108	T1422	T1423	amod	independent,mechanisms
R1109	T1423	T1417	pobj	mechanisms,through
R1110	T1424	T1423	appos	.6,mechanisms
R1111	T1425	T1427	nummod	8,activation
R1112	T1426	T1427	amod	LPS-induced,activation
R1113	T1427	T1439	nsubj	activation,been
R1114	T1428	T1427	prep	of,activation
R1115	T1429	T1431	det	the,factor
R1116	T1430	T1431	compound	transcription,factor
R1117	T1431	T1428	pobj	factor,of
R1118	T1432	T1433	amod	nuclear,factor-κB
R1119	T1433	T1439	nsubj	factor-κB,been
R1120	T1434	T1435	punct	(,NF-κB
R1121	T1435	T1433	appos	NF-κB,factor-κB
R1122	T1436	T1433	punct	),factor-κB
R1123	T1437	T1439	aux	has,been
R1124	T1438	T1439	advmod	also,been
R1125	T1439	T1406	conj	been,is
R1126	T1440	T1441	det	the,focus
R1127	T1441	T1439	attr	focus,been
R1128	T1442	T1441	prep	of,focus
R1129	T1443	T1445	det	a,deal
R1130	T1444	T1445	amod	great,deal
R1131	T1445	T1442	pobj	deal,of
R1132	T1446	T1445	prep	of,deal
R1133	T1447	T1446	pobj	research,of
R1134	T1448	T1439	punct	.,been
R1135	T1449	T1453	nsubjpass	It,demonstrated
R1136	T1450	T1453	aux	has,demonstrated
R1137	T1451	T1453	auxpass	been,demonstrated
R1138	T1452	T1453	advmod	clearly,demonstrated
R1139	T1453	T1453	ROOT	demonstrated,demonstrated
R1140	T1454	T1461	mark	that,inhibit
R1141	T1455	T1461	nsubj	agents,inhibit
R1142	T1456	T1457	nsubj	that,increase
R1143	T1457	T1455	relcl	increase,agents
R1144	T1458	T1459	compound	intracellular,cAMP
R1145	T1459	T1457	dobj	cAMP,increase
R1146	T1460	T1461	advmod	also,inhibit
R1147	T1461	T1453	ccomp	inhibit,demonstrated
R1148	T1462	T1465	amod	NF-κB-dependent,transcription
R1149	T1463	T1465	amod	pro-inflammatory,transcription
R1150	T1464	T1465	compound	gene,transcription
R1151	T1465	T1461	dobj	transcription,inhibit
R1152	T1466	T1465	punct	",",transcription
R1153	T1467	T1468	advmod	particularly,of
R1154	T1468	T1465	prep	of,transcription
R1155	T1469	T1471	det	the,gene
R1156	T1470	T1471	compound	TNF-α,gene
R1157	T1471	T1468	pobj	gene,of
R1158	T1472	T1473	nummod	.9,Controversy
R1159	T1473	T1474	nsubj	Controversy,exists
R1160	T1474	T1465	relcl	exists,transcription
R1161	T1475	T1474	prep	regarding,exists
R1162	T1476	T1478	det	the,mechanism
R1163	T1477	T1478	amod	exact,mechanism
R1164	T1478	T1475	pobj	mechanism,regarding
R1165	T1479	T1478	punct	(,mechanism
R1166	T1480	T1478	appos	s,mechanism
R1167	T1481	T1478	punct	),mechanism
R1168	T1482	T1486	prep	by,down-regulates
R1169	T1483	T1482	pobj	which,by
R1170	T1484	T1486	nmod	PDE,down-regulates
R1171	T1485	T1486	compound	inhibition,down-regulates
R1172	T1486	T1474	conj	down-regulates,exists
R1173	T1487	T1488	amod	TNF-α,production
R1174	T1488	T1486	dobj	production,down-regulates
R1175	T1489	T1453	punct	.,demonstrated
R1176	T1490	T1491	nsubj	Possibilities,include
R1177	T1491	T1491	ROOT	include,include
R1178	T1492	T1491	punct	",",include
R1179	T1493	T1491	cc	but,include
R1180	T1494	T1496	auxpass	are,limited
R1181	T1495	T1496	neg	not,limited
R1182	T1496	T1491	conj	limited,include
R1183	T1497	T1496	prep	to,limited
R1184	T1498	T1497	punct	",",to
R1185	T1499	T1497	pobj	inhibition,to
R1186	T1500	T1499	prep	of,inhibition
R1187	T1501	T1502	compound	NF-κB,DNA
R1188	T1502	T1500	pobj	DNA,of
R1189	T1503	T1504	amod	binding,activity
R1190	T1504	T1499	conj	activity,inhibition
R1191	T1505	T1499	punct	",",inhibition
R1192	T1506	T1499	appos	downregulation,inhibition
R1193	T1507	T1506	prep	of,downregulation
R1194	T1508	T1510	amod	NF-κB,activity
R1195	T1509	T1510	amod	transcriptional,activity
R1196	T1510	T1507	pobj	activity,of
R1197	T1511	T1512	punct	",",increased
R1198	T1512	T1514	amod	increased,activation
R1199	T1513	T1514	compound	CREB,activation
R1200	T1514	T1496	dobj	activation,limited
R1201	T1515	T1514	punct	",",activation
R1202	T1516	T1514	cc	and,activation
R1203	T1517	T1514	conj	competition,activation
R1204	T1518	T1514	prep	between,activation
R1205	T1519	T1518	pobj	NF-κB,between
R1206	T1520	T1519	cc	and,NF-κB
R1207	T1521	T1519	conj	CREB,NF-κB
R1208	T1522	T1514	prep	for,activation
R1209	T1523	T1524	amod	common,co-activators
R1210	T1524	T1522	pobj	co-activators,for
R1211	T1525	T1526	amod	such,as
R1212	T1526	T1524	prep	as,co-activators
R1213	T1527	T1526	pobj	CREB,as
R1214	T1528	T1529	amod	binding,protein
R1215	T1529	T1527	appos	protein,CREB
R1216	T1530	T1531	punct	(,CBP
R1217	T1531	T1529	appos	CBP,protein
R1218	T1532	T1527	punct	),CREB
R1219	T1533	T1491	punct	.,include
R1220	T1534	T1535	nsubj	It,is
R1221	T1535	T1538	auxpass	is,known
R1222	T1536	T1535	advmod	also,is
R1223	T1537	T1538	neg	not,known
R1224	T1538	T1538	ROOT	known,known
R1225	T1539	T1542	mark	whether,rely
R1226	T1540	T1541	det	these,processes
R1227	T1541	T1542	nsubj	processes,rely
R1228	T1542	T1538	ccomp	rely,known
R1229	T1543	T1544	advmod	solely,upon
R1230	T1544	T1542	prep	upon,rely
R1231	T1545	T1546	det	the,activation
R1232	T1546	T1544	pobj	activation,upon
R1233	T1547	T1546	prep	of,activation
R1234	T1548	T1547	pobj	PKA,of
R1235	T1549	T1538	punct	.,known
R1236	T1550	T1558	advmod	Therefore,is
R1237	T1551	T1558	punct	",",is
R1238	T1552	T1553	det	the,objective
R1239	T1553	T1558	nsubj	objective,is
R1240	T1554	T1553	prep	of,objective
R1241	T1555	T1557	det	the,study
R1242	T1556	T1557	amod	present,study
R1243	T1557	T1554	pobj	study,of
R1244	T1558	T1558	ROOT	is,is
R1245	T1559	T1560	aux	to,determine
R1246	T1560	T1558	xcomp	determine,is
R1247	T1561	T1562	det	the,effects
R1248	T1562	T1560	dobj	effects,determine
R1249	T1563	T1562	prep	of,effects
R1250	T1564	T1566	amod	nonspecific,inhibition
R1251	T1565	T1566	compound	PDE,inhibition
R1252	T1566	T1563	pobj	inhibition,of
R1253	T1567	T1562	prep	with,effects
R1254	T1568	T1570	meta	1,[
R1255	T1569	T1570	punct	-,[
R1256	T1570	T1572	compound	[,]
R1257	T1571	T1572	compound	5-oxohexyl,]
R1258	T1572	T1567	pobj	],with
R1259	T1573	T1575	nummod	"-3,7",dimethylxanthine
R1260	T1574	T1575	punct	-,dimethylxanthine
R1261	T1575	T1572	appos	dimethylxanthine,]
R1262	T1576	T1577	punct	(,Pentoxifylline
R1263	T1577	T1575	dep	Pentoxifylline,dimethylxanthine
R1264	T1578	T1577	punct	;,Pentoxifylline
R1265	T1579	T1577	appos	PTX,Pentoxifylline
R1266	T1580	T1577	punct	),Pentoxifylline
R1267	T1581	T1577	prep	on,Pentoxifylline
R1268	T1582	T1581	pobj	NF-κB,on
R1269	T1583	T1582	cc	and,NF-κB
R1270	T1584	T1585	compound	CREB,activation
R1271	T1585	T1582	conj	activation,NF-κB
R1272	T1586	T1585	prep	in,activation
R1273	T1587	T1586	pobj	vitro,in
R1274	T1588	T1585	prep	in,activation
R1275	T1589	T1591	amod	human,cells
R1276	T1590	T1591	compound	mononuclear,cells
R1277	T1591	T1588	pobj	cells,in
R1278	T1592	T1558	punct	.,is
R1279	T1593	T1602	prep	With,investigated
R1280	T1594	T1595	det	the,use
R1281	T1595	T1593	pobj	use,With
R1282	T1596	T1595	prep	of,use
R1283	T1597	T1598	amod	specific,inhibitors
R1284	T1598	T1596	pobj	inhibitors,of
R1285	T1599	T1602	punct	",",investigated
R1286	T1600	T1602	nsubj	we,investigated
R1287	T1601	T1602	advmod	also,investigated
R1288	T1602	T1602	ROOT	investigated,investigated
R1289	T1603	T1604	det	the,role
R1290	T1604	T1602	dobj	role,investigated
R1291	T1605	T1604	prep	of,role
R1292	T1606	T1605	pobj	PKA,of
R1293	T1607	T1604	prep	in,role
R1294	T1608	T1610	amod	LPS-induced,production
R1295	T1609	T1610	compound	TNF-α,production
R1296	T1610	T1607	pobj	production,in
R1297	T1611	T1602	punct	.,investigated
R1891	T2464	T2465	det	this,study
R1892	T2465	T2467	nsubjpass	study,approved
R1893	T2466	T2467	auxpass	was,approved
R1894	T2467	T2467	ROOT	approved,approved
R1895	T2468	T2467	agent	by,approved
R1896	T2469	T2473	det	the,Program
R1897	T2470	T2472	compound	Human,Protections
R1898	T2471	T2472	compound	Research,Protections
R1899	T2472	T2473	compound	Protections,Program
R1900	T2473	T2468	pobj	Program,by
R1901	T2474	T2473	cc	and,Program
R1902	T2475	T2478	det	the,Board
R1903	T2476	T2478	compound	Institutional,Board
R1904	T2477	T2478	compound	Review,Board
R1905	T2478	T2473	conj	Board,Program
R1906	T2479	T2467	punct	.,approved
R1907	T2480	T2481	amod	Written,consent
R1908	T2481	T2488	nsubjpass	consent,obtained
R1909	T2482	T2483	aux	to,participate
R1910	T2483	T2481	relcl	participate,consent
R1911	T2484	T2483	prep	in,participate
R1912	T2485	T2486	det	the,study
R1913	T2486	T2484	pobj	study,in
R1914	T2487	T2488	auxpass	was,obtained
R1915	T2488	T2488	ROOT	obtained,obtained
R1916	T2489	T2488	prep	from,obtained
R1917	T2490	T2491	det	all,volunteers
R1918	T2491	T2489	pobj	volunteers,from
R1919	T2492	T2488	advmod	prior,obtained
R1920	T2493	T2492	prep	to,prior
R1921	T2494	T2493	pobj	blood,to
R1922	T2495	T2496	amod	"donation. Heparinized",Vacutainers
R1923	T2496	T2493	pobj	Vacutainers,to
R1924	T2497	T2498	auxpass	were,purchased
R1925	T2498	T2488	conj	purchased,obtained
R1926	T2499	T2498	prep	from,purchased
R1927	T2500	T2501	compound	Becton,Dickinson
R1928	T2501	T2499	pobj	Dickinson,from
R1929	T2502	T2501	punct	(,Dickinson
R1930	T2503	T2504	compound	San,Jose
R1931	T2504	T2501	appos	Jose,Dickinson
R1932	T2505	T2504	punct	",",Jose
R1933	T2506	T2501	conj	CA,Dickinson
R1934	T2507	T2488	punct	),obtained
R1935	T2508	T2488	punct	.,obtained
R100	T202	T200	pobj	sepsis,in
R101	T203	T202	cc	and,sepsis
R102	T204	T202	conj	inflammation,sepsis
R339	T441	T443	det	a,tool
R340	T442	T443	amod	useful,tool
R341	T443	T440	pobj	tool,as
R342	T444	T443	prep	in,tool
R343	T445	T446	det	the,attenuation
R344	T446	T444	pobj	attenuation,in
R345	T447	T446	prep	of,attenuation
R346	T448	T449	amod	LPS-induced,inflammation
R347	T449	T447	pobj	inflammation,of
R925	T1239	T1223	conj	is,shown
R926	T1240	T1243	det	a,cytokine
R927	T1241	T1243	amod	potent,cytokine
R928	T1242	T1243	amod	pro-inflammatory,cytokine
R929	T1243	T1239	attr	cytokine,is
R930	T1244	T1243	acl	involved,cytokine
R931	T1245	T1244	prep	in,involved
R932	T1246	T1248	det	a,spectrum
R933	T1247	T1248	amod	wide,spectrum
R934	T1248	T1245	pobj	spectrum,in
R935	T1249	T1248	prep	of,spectrum
R936	T1250	T1251	amod	cellular,responses
R937	T1251	T1249	pobj	responses,of
R1095	T1409	T1406	attr	body,is
R1096	T1410	T1409	prep	of,body
R1097	T1411	T1410	pobj	evidence,of
R1098	T1412	T1411	acl	suggesting,evidence
R1099	T1413	T1416	mark	that,function
R60	T162	T159	pobj	inhibition,of
R61	T163	T162	prep	on,inhibition
R62	T164	T165	compound	transcription,factor
R63	T165	T166	compound	factor,activation
R64	T166	T163	pobj	activation,on
R65	T167	T166	cc	and,activation
R66	T168	T169	compound	tumor,necrosis
R67	T169	T170	compound	necrosis,factor-alpha
R68	T170	T166	conj	factor-alpha,activation
R69	T171	T172	punct	(,TNF-α
R70	T172	T170	appos	TNF-α,factor-alpha
R71	T173	T170	punct	),factor-alpha
R72	T174	T166	conj	production,activation
R73	T175	T174	prep	in,production
R74	T176	T175	pobj	lipopolysaccharide,in
R75	T177	T178	punct	(,LPS
R76	T178	T176	appos	LPS,lipopolysaccharide
R77	T179	T176	punct	),lipopolysaccharide

UBERON-AE

Id	Subject	Object	Predicate	Lexical cue
T1125	2297-2310	http://purl.obolibrary.org/obo/UBERON_0002405	denotes	immune system
T2438	4959-4964	http://purl.obolibrary.org/obo/UBERON_0000178	denotes	blood

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T826	151-168	http://purl.obolibrary.org/obo/GO_0032680	denotes	TNF-α) production
T827	251-270	http://purl.obolibrary.org/obo/GO_0032680	denotes	production of TNF-α
T828	418-434	http://purl.obolibrary.org/obo/GO_0032680	denotes	TNF-α production
T834	151-168	http://purl.obolibrary.org/obo/GO_0032640	denotes	TNF-α) production
T835	251-270	http://purl.obolibrary.org/obo/GO_0032640	denotes	production of TNF-α
T836	251-268	http://purl.obolibrary.org/obo/GO_0032640	denotes	production of TNF
T838	54-71	http://purl.obolibrary.org/obo/GO_0008081	denotes	phosphodiesterase
T839	382-399	http://purl.obolibrary.org/obo/GO_0008081	denotes	phosphodiesterase
T840	618-635	http://purl.obolibrary.org/obo/GO_0008081	denotes	phosphodiesterase
T841	1500-1517	http://purl.obolibrary.org/obo/GO_0008081	denotes	phosphodiesterase
T842	1753-1770	http://purl.obolibrary.org/obo/GO_0008081	denotes	phosphodiesterase
T843	72-106	http://purl.obolibrary.org/obo/GO_1901484	denotes	inhibition on transcription factor
T844	72-106	http://purl.obolibrary.org/obo/GO_0043433	denotes	inhibition on transcription factor
T845	72-106	http://purl.obolibrary.org/obo/GO_0032792	denotes	inhibition on transcription factor
T846	86-99	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T847	1634-1649	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcriptional
T848	86-117	http://purl.obolibrary.org/obo/GO_0051091	denotes	transcription factor activation
T849	86-117	http://purl.obolibrary.org/obo/GO_0032793	denotes	transcription factor activation
T850	1615-1633	http://purl.obolibrary.org/obo/GO_0032793	denotes	activation of CREB
T851	86-117	http://purl.obolibrary.org/obo/GO_1901485	denotes	transcription factor activation
T852	128-136	http://purl.obolibrary.org/obo/GO_0001906	denotes	necrosis
T853	128-136	http://purl.obolibrary.org/obo/GO_0008219	denotes	necrosis
T854	128-136	http://purl.obolibrary.org/obo/GO_0019835	denotes	necrosis
T855	128-136	http://purl.obolibrary.org/obo/GO_0070265	denotes	necrosis
T856	345-357	http://purl.obolibrary.org/obo/GO_0006954	denotes	inflammation
T857	1911-1923	http://purl.obolibrary.org/obo/GO_0006954	denotes	inflammation
T858	618-645	http://purl.obolibrary.org/obo/GO_0051344	denotes	phosphodiesterase inhibitor
T859	744-757	http://purl.obolibrary.org/obo/GO_0051591	denotes	cAMP-response
T860	1133-1148	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T861	1162-1177	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T862	1257-1272	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T863	1601-1625	http://purl.obolibrary.org/obo/GO_0051092	denotes	NF-κB and the activation
T2109	2023-2038	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T2110	3137-3152	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T2111	2023-2053	http://purl.obolibrary.org/obo/GO_0042327	denotes	phosphorylation and activation
T2112	2071-2100	http://purl.obolibrary.org/obo/GO_0035556	denotes	intracellular protein kinases
T2113	2105-2118	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T2114	2201-2214	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T2115	2327-2340	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T2116	3160-3173	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T2117	3307-3320	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T2118	3671-3684	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T2119	3900-3913	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T2120	4170-4185	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcriptional
T2121	2128-2159	http://purl.obolibrary.org/obo/GO_0051091	denotes	Transcription factor activation
T2122	2327-2358	http://purl.obolibrary.org/obo/GO_0051091	denotes	transcription factor activation
T2123	3653-3691	http://purl.obolibrary.org/obo/GO_0051091	denotes	activation of the transcription factor
T2124	2128-2159	http://purl.obolibrary.org/obo/GO_0032793	denotes	Transcription factor activation
T2125	2327-2358	http://purl.obolibrary.org/obo/GO_0032793	denotes	transcription factor activation
T2126	3653-3691	http://purl.obolibrary.org/obo/GO_0032793	denotes	activation of the transcription factor
T2127	4206-4221	http://purl.obolibrary.org/obo/GO_0032793	denotes	CREB activation
T2128	4588-4603	http://purl.obolibrary.org/obo/GO_0032793	denotes	CREB activation
T2129	2128-2159	http://purl.obolibrary.org/obo/GO_1901485	denotes	Transcription factor activation
T2130	2327-2358	http://purl.obolibrary.org/obo/GO_1901485	denotes	transcription factor activation
T2131	3653-3691	http://purl.obolibrary.org/obo/GO_1901485	denotes	activation of the transcription factor
T2132	2219-2236	http://purl.obolibrary.org/obo/GO_0006412	denotes	protein synthesis
T2133	2227-2236	http://purl.obolibrary.org/obo/GO_0009058	denotes	synthesis
T2134	2558-2567	http://purl.obolibrary.org/obo/GO_0009058	denotes	synthesis
T2135	2405-2413	http://purl.obolibrary.org/obo/GO_0001906	denotes	necrosis
T2136	2405-2413	http://purl.obolibrary.org/obo/GO_0008219	denotes	necrosis
T2137	2405-2413	http://purl.obolibrary.org/obo/GO_0019835	denotes	necrosis
T2138	2405-2413	http://purl.obolibrary.org/obo/GO_0070265	denotes	necrosis
T2139	2424-2441	http://purl.obolibrary.org/obo/GO_0032640	denotes	TNF-α) production
T2140	2810-2826	http://purl.obolibrary.org/obo/GO_0032640	denotes	TNF-α production
T2141	4039-4055	http://purl.obolibrary.org/obo/GO_0032640	denotes	TNF-α production
T2142	4730-4746	http://purl.obolibrary.org/obo/GO_0032640	denotes	TNF-α production
T2143	2424-2441	http://purl.obolibrary.org/obo/GO_0032680	denotes	TNF-α) production
T2144	2810-2826	http://purl.obolibrary.org/obo/GO_0032680	denotes	TNF-α production
T2145	4039-4055	http://purl.obolibrary.org/obo/GO_0032680	denotes	TNF-α production
T2146	4730-4746	http://purl.obolibrary.org/obo/GO_0032680	denotes	TNF-α production
T2147	2519-2527	http://purl.obolibrary.org/obo/GO_0007349	denotes	cellular
T2148	3492-3500	http://purl.obolibrary.org/obo/GO_0007349	denotes	cellular
T2149	2613-2630	http://purl.obolibrary.org/obo/GO_0008081	denotes	phosphodiesterase
T2150	2632-2635	http://purl.obolibrary.org/obo/GO_0004114	denotes	PDE
T2151	2747-2750	http://purl.obolibrary.org/obo/GO_0004114	denotes	PDE
T2152	2927-2930	http://purl.obolibrary.org/obo/GO_0004114	denotes	PDE
T2153	4009-4012	http://purl.obolibrary.org/obo/GO_0004114	denotes	PDE
T2154	4497-4500	http://purl.obolibrary.org/obo/GO_0004114	denotes	PDE
T2155	2714-2723	http://purl.obolibrary.org/obo/GO_0023052	denotes	signaling
T2156	3243-3250	http://purl.obolibrary.org/obo/GO_0023052	denotes	signals
T2157	2792-2815	http://purl.obolibrary.org/obo/GO_1904468	denotes	downregulation of TNF-α
T2158	3065-3093	http://purl.obolibrary.org/obo/GO_0034199	denotes	activation of Protein kinase
T2159	3065-3093	http://purl.obolibrary.org/obo/GO_0032147	denotes	activation of Protein kinase
T2160	3065-3093	http://purl.obolibrary.org/obo/GO_0030295	denotes	activation of Protein kinase
T2161	3097-3100	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T2162	3104-3107	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T2163	3595-3598	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T2164	4403-4406	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T2165	4711-4714	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T2166	3181-3194	http://purl.obolibrary.org/obo/GO_0051591	denotes	cAMP-response
T2167	3854-3867	http://purl.obolibrary.org/obo/GO_0032088	denotes	inhibit NF-κB
T2168	3854-3867	http://purl.obolibrary.org/obo/GO_0007252	denotes	inhibit NF-κB
T3127	5047-5049	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T865	54-71	http://purl.obolibrary.org/obo/GO_0008081	denotes	phosphodiesterase
T866	382-399	http://purl.obolibrary.org/obo/GO_0008081	denotes	phosphodiesterase
T867	618-635	http://purl.obolibrary.org/obo/GO_0008081	denotes	phosphodiesterase
T868	1500-1517	http://purl.obolibrary.org/obo/GO_0008081	denotes	phosphodiesterase
T869	1753-1770	http://purl.obolibrary.org/obo/GO_0008081	denotes	phosphodiesterase
T870	766-773	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T871	808-815	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T872	1191-1198	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T873	1281-1288	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T874	1654-1661	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T875	766-781	http://purl.obolibrary.org/obo/GO_0005515	denotes	binding protein
T876	804-815	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T877	1187-1198	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T878	1277-1288	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T879	1650-1661	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T880	808-824	http://purl.obolibrary.org/obo/GO_0051059	denotes	binding of NF-κB
T2192	2327-2358	http://purl.obolibrary.org/obo/GO_0033613	denotes	transcription factor activation
T2193	3653-3691	http://purl.obolibrary.org/obo/GO_0033613	denotes	activation of the transcription factor
T2194	2613-2630	http://purl.obolibrary.org/obo/GO_0008081	denotes	phosphodiesterase
T2195	2632-2635	http://purl.obolibrary.org/obo/GO_0004114	denotes	PDE
T2196	2747-2750	http://purl.obolibrary.org/obo/GO_0004114	denotes	PDE
T2197	2927-2930	http://purl.obolibrary.org/obo/GO_0004114	denotes	PDE
T2198	4009-4012	http://purl.obolibrary.org/obo/GO_0004114	denotes	PDE
T2199	4497-4500	http://purl.obolibrary.org/obo/GO_0004114	denotes	PDE
T2200	3065-3093	http://purl.obolibrary.org/obo/GO_0030295	denotes	activation of Protein kinase
T2201	3097-3100	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T2202	3104-3107	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T2203	3595-3598	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T2204	4403-4406	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T2205	4711-4714	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T2206	3203-3210	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T2207	4128-4135	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T2208	4300-4307	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T2209	3203-3218	http://purl.obolibrary.org/obo/GO_0005515	denotes	binding protein
T2210	4300-4315	http://purl.obolibrary.org/obo/GO_0005515	denotes	binding protein
T2211	4124-4135	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T2212	4295-4307	http://purl.obolibrary.org/obo/GO_0008140	denotes	CREB binding
T2213	4317-4320	http://purl.obolibrary.org/obo/GO_0008140	denotes	CBP
T3144	5047-5049	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T881	226-231	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T882	502-507	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T883	950-955	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T2214	1954-1962	http://purl.obolibrary.org/obo/GO_0016020	denotes	membrane
T2215	2066-2070	http://purl.obolibrary.org/obo/GO_0018995	denotes	host
T2216	2292-2296	http://purl.obolibrary.org/obo/GO_0018995	denotes	host
T2217	2066-2084	http://purl.obolibrary.org/obo/GO_0043656	denotes	host intracellular
T2218	2071-2084	http://purl.obolibrary.org/obo/GO_0005622	denotes	intracellular
T2219	2957-2970	http://purl.obolibrary.org/obo/GO_0005622	denotes	intracellular
T2220	3427-3440	http://purl.obolibrary.org/obo/GO_0005622	denotes	intracellular
T2221	3830-3843	http://purl.obolibrary.org/obo/GO_0005622	denotes	intracellular
T2222	2596-2601	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T2223	2877-2882	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T2224	4634-4639	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T2225	3097-3100	http://purl.obolibrary.org/obo/GO_0005952	denotes	PKA
T2226	3104-3107	http://purl.obolibrary.org/obo/GO_0005952	denotes	PKA
T2227	3595-3598	http://purl.obolibrary.org/obo/GO_0005952	denotes	PKA
T2228	4403-4406	http://purl.obolibrary.org/obo/GO_0005952	denotes	PKA
T2229	4711-4714	http://purl.obolibrary.org/obo/GO_0005952	denotes	PKA
T2230	3260-3267	http://purl.obolibrary.org/obo/GO_0005634	denotes	nucleus
T2231	3671-3699	http://purl.obolibrary.org/obo/GO_0044798	denotes	transcription factor nuclear

sentences

Id	Subject	Object	Predicate	Lexical cue
T114	234-246	Sentence	denotes	INTRODUCTION
T115	247-358	Sentence	denotes	The production of TNF-α following LPS stimulation is one of the key steps in bacterial sepsis and inflammation.
T116	359-474	Sentence	denotes	The mechanism by which phosphodiesterase inhibition alters TNF-α production in the presence of LPS remains unclear.
T117	476-483	Sentence	denotes	METHODS
T118	484-646	Sentence	denotes	Human mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor.
T119	647-803	Sentence	denotes	Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed.
T120	804-887	Sentence	denotes	DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay.
T121	888-1060	Sentence	denotes	TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA).
T122	1062-1069	Sentence	denotes	RESULTS
T123	1070-1217	Sentence	denotes	PTX was demonstrated to significantly reduce cytoplasmic I-κBα phosphorylation, nuclear p65 phosphorylation, and the DNA binding activity of NF-κB.
T124	1218-1306	Sentence	denotes	In contrast, PTX markedly enhanced the phosphorylation and DNA binding activity of CREB.
T125	1307-1441	Sentence	denotes	Cells concomitantly treated with PTX and LPS secreted similar levels of TNF-α in the presence and absence Protein kinase A inhibition.
T126	1443-1453	Sentence	denotes	DISCUSSION
T127	1454-1727	Sentence	denotes	The increased level of cAMP that results from phosphodiesterase inhibition affects cytoplasmic and nuclear events, resulting in the attenuation of NF-κB and the activation of CREB transcriptional DNA binding through pathways that are partially Protein kinase A-independent.
T128	1729-1739	Sentence	denotes	CONCLUSION
T129	1740-1924	Sentence	denotes	PTX-mediated phosphodiesterase inhibition occurs partially through a Protein kinase A-independent pathway and may serve as a useful tool in the attenuation of LPS-induced inflammation.
T1149	1940-2127	Sentence	denotes	The bacterial membrane component lipopolysaccharide (LPS) is capable of initiating phosphorylation and activation of multiple host intracellular protein kinases and transcription factors.
T1150	2128-2314	Sentence	denotes	Transcription factor activation, which results in the modulation of gene transcription and protein synthesis, is a critical element in the defense mechanism of the host immune system.1,2
T1151	2315-2538	Sentence	denotes	LPS-induced transcription factor activation has been shown to be a key regulator of tumor necrosis factor-α (TNF-α) production.3 TNF-α is a potent pro-inflammatory cytokine involved in a wide spectrum of cellular responses.
T1152	2539-2883	Sentence	denotes	Furthermore, TNF-α synthesis can be attenuated in immune cells exposed to phosphodiesterase (PDE) inhibition after challenge by a variety of pro-inflammatory stimulants.4 The signaling mechanisms affected by PDE inhibition, which ultimately lead to the downregulation of TNF-α production, have not been well characterized in inflammatory cells.
T1153	2884-3511	Sentence	denotes	Classically, it has been demonstrated that PDE inhibition results in the intracellular accumulation of the second messenger cyclic adenosine-3,5-monophosphate (cAMP) and subsequent activation of Protein kinase A (PKA).5 PKA activation then leads to the phosphorylation of the transcription factor cAMP-response element binding protein (CREB), transmission of signals into the nucleus, and the subsequent modulation of gene transcription.6 This apparently simple linear cascade does not fully explain the mechanism by which an elevation in the intracellular cAMP level exerts wide-ranging effects on multiple cellular functions.
T1154	3512-3640	Sentence	denotes	There is a growing body of evidence suggesting that cAMP may function through both PKA-dependent and -independent mechanisms.6–8
T1155	3641-3770	Sentence	denotes	LPS-induced activation of the transcription factor nuclear factor-κB (NF-κB) has also been the focus of a great deal of research.
T1156	3771-3947	Sentence	denotes	It has been clearly demonstrated that agents that increase intracellular cAMP also inhibit NF-κB-dependent pro-inflammatory gene transcription, particularly of the TNF-α gene.9
T1157	3948-4056	Sentence	denotes	Controversy exists regarding the exact mechanism(s) by which PDE inhibition down-regulates TNF-α production.
T1158	4057-4322	Sentence	denotes	Possibilities include, but are not limited to, inhibition of NF-κB DNA binding activity, downregulation of NF-κB transcriptional activity, increased CREB activation, and competition between NF-κB and CREB for common co-activators such as CREB binding protein (CBP).
T1159	4323-4407	Sentence	denotes	It is also not known whether these processes rely solely upon the activation of PKA.
T1160	4408-4640	Sentence	denotes	Therefore, the objective of the present study is to determine the effects of nonspecific PDE inhibition with 1-[5-oxohexyl]-3,7-dimethylxanthine (Pentoxifylline; PTX) on NF-κB and CREB activation in vitro in human mononuclear cells.
T1161	4641-4747	Sentence	denotes	With the use of specific inhibitors, we also investigated the role of PKA in LPS-induced TNF-α production.
T2453	0-5051	Sentence	denotes	this study was to determine the effect of nonspecific phosphodiesterase inhibition on transcription factor activation and tumor necrosis factor-alpha (TNF-α) production in lipopolysaccharide (LPS)-stimulated human mononuclear cells. INTRODUCTION The production of TNF-α following LPS stimulation is one of the key steps in bacterial sepsis and inflammation. The mechanism by which phosphodiesterase inhibition alters TNF-α production in the presence of LPS remains unclear. METHODS Human mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor. Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed. DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay. TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA). RESULTS PTX was demonstrated to significantly reduce cytoplasmic I-κBα phosphorylation, nuclear p65 phosphorylation, and the DNA binding activity of NF-κB. In contrast, PTX markedly enhanced the phosphorylation and DNA binding activity of CREB. Cells concomitantly treated with PTX and LPS secreted similar levels of TNF-α in the presence and absence Protein kinase A inhibition. DISCUSSION The increased level of cAMP that results from phosphodiesterase inhibition affects cytoplasmic and nuclear events, resulting in the attenuation of NF-κB and the activation of CREB transcriptional DNA binding through pathways that are partially Protein kinase A-independent. CONCLUSION PTX-mediated phosphodiesterase inhibition occurs partially through a Protein kinase A-independent pathway and may serve as a useful tool in the attenuation of LPS-induced inflammation. INTRODUCTION The bacterial membrane component lipopolysaccharide (LPS) is capable of initiating phosphorylation and activation of multiple host intracellular protein kinases and transcription factors. Transcription factor activation, which results in the modulation of gene transcription and protein synthesis, is a critical element in the defense mechanism of the host immune system.1,2 LPS-induced transcription factor activation has been shown to be a key regulator of tumor necrosis factor-α (TNF-α) production.3 TNF-α is a potent pro-inflammatory cytokine involved in a wide spectrum of cellular responses. Furthermore, TNF-α synthesis can be attenuated in immune cells exposed to phosphodiesterase (PDE) inhibition after challenge by a variety of pro-inflammatory stimulants.4 The signaling mechanisms affected by PDE inhibition, which ultimately lead to the downregulation of TNF-α production, have not been well characterized in inflammatory cells. Classically, it has been demonstrated that PDE inhibition results in the intracellular accumulation of the second messenger cyclic adenosine-3,5-monophosphate (cAMP) and subsequent activation of Protein kinase A (PKA).5 PKA activation then leads to the phosphorylation of the transcription factor cAMP-response element binding protein (CREB), transmission of signals into the nucleus, and the subsequent modulation of gene transcription.6 This apparently simple linear cascade does not fully explain the mechanism by which an elevation in the intracellular cAMP level exerts wide-ranging effects on multiple cellular functions. There is a growing body of evidence suggesting that cAMP may function through both PKA-dependent and -independent mechanisms.6–8 LPS-induced activation of the transcription factor nuclear factor-κB (NF-κB) has also been the focus of a great deal of research. It has been clearly demonstrated that agents that increase intracellular cAMP also inhibit NF-κB-dependent pro-inflammatory gene transcription, particularly of the TNF-α gene.9 Controversy exists regarding the exact mechanism(s) by which PDE inhibition down-regulates TNF-α production. Possibilities include, but are not limited to, inhibition of NF-κB DNA binding activity, downregulation of NF-κB transcriptional activity, increased CREB activation, and competition between NF-κB and CREB for common co-activators such as CREB binding protein (CBP). It is also not known whether these processes rely solely upon the activation of PKA. Therefore, the objective of the present study is to determine the effects of nonspecific PDE inhibition with 1-[5-oxohexyl]-3,7-dimethylxanthine (Pentoxifylline; PTX) on NF-κB and CREB activation in vitro in human mononuclear cells. With the use of specific inhibitors, we also investigated the role of PKA in LPS-induced TNF-α production. MATERIALS AND METHODS This study was approved by the Human Research Protections Program and the Institutional Review Board. Written consent to participate in the study was obtained from all volunteers prior to blood donation. Heparinized Vacutainers were purchased from Becton Dickinson (San Jose, CA).
T6	234-246	Sentence	denotes	INTRODUCTION
T7	247-358	Sentence	denotes	The production of TNF-α following LPS stimulation is one of the key steps in bacterial sepsis and inflammation.
T8	359-474	Sentence	denotes	The mechanism by which phosphodiesterase inhibition alters TNF-α production in the presence of LPS remains unclear.
T9	476-483	Sentence	denotes	METHODS
T10	484-646	Sentence	denotes	Human mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor.
T11	647-803	Sentence	denotes	Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed.
T12	804-887	Sentence	denotes	DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay.
T13	888-1060	Sentence	denotes	TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA).
T14	1062-1069	Sentence	denotes	RESULTS
T15	1070-1217	Sentence	denotes	PTX was demonstrated to significantly reduce cytoplasmic I-κBα phosphorylation, nuclear p65 phosphorylation, and the DNA binding activity of NF-κB.
T16	1218-1306	Sentence	denotes	In contrast, PTX markedly enhanced the phosphorylation and DNA binding activity of CREB.
T17	1307-1441	Sentence	denotes	Cells concomitantly treated with PTX and LPS secreted similar levels of TNF-α in the presence and absence Protein kinase A inhibition.
T18	1443-1453	Sentence	denotes	DISCUSSION
T19	1454-1727	Sentence	denotes	The increased level of cAMP that results from phosphodiesterase inhibition affects cytoplasmic and nuclear events, resulting in the attenuation of NF-κB and the activation of CREB transcriptional DNA binding through pathways that are partially Protein kinase A-independent.
T20	1729-1739	Sentence	denotes	CONCLUSION
T21	1740-1924	Sentence	denotes	PTX-mediated phosphodiesterase inhibition occurs partially through a Protein kinase A-independent pathway and may serve as a useful tool in the attenuation of LPS-induced inflammation.
T22	1927-1939	Sentence	denotes	INTRODUCTION
T23	1940-2127	Sentence	denotes	The bacterial membrane component lipopolysaccharide (LPS) is capable of initiating phosphorylation and activation of multiple host intracellular protein kinases and transcription factors.
T24	2128-2314	Sentence	denotes	Transcription factor activation, which results in the modulation of gene transcription and protein synthesis, is a critical element in the defense mechanism of the host immune system.1,2
T25	2315-2538	Sentence	denotes	LPS-induced transcription factor activation has been shown to be a key regulator of tumor necrosis factor-α (TNF-α) production.3 TNF-α is a potent pro-inflammatory cytokine involved in a wide spectrum of cellular responses.
T26	2539-2883	Sentence	denotes	Furthermore, TNF-α synthesis can be attenuated in immune cells exposed to phosphodiesterase (PDE) inhibition after challenge by a variety of pro-inflammatory stimulants.4 The signaling mechanisms affected by PDE inhibition, which ultimately lead to the downregulation of TNF-α production, have not been well characterized in inflammatory cells.
T27	2884-3511	Sentence	denotes	Classically, it has been demonstrated that PDE inhibition results in the intracellular accumulation of the second messenger cyclic adenosine-3,5-monophosphate (cAMP) and subsequent activation of Protein kinase A (PKA).5 PKA activation then leads to the phosphorylation of the transcription factor cAMP-response element binding protein (CREB), transmission of signals into the nucleus, and the subsequent modulation of gene transcription.6 This apparently simple linear cascade does not fully explain the mechanism by which an elevation in the intracellular cAMP level exerts wide-ranging effects on multiple cellular functions.
T28	3512-3640	Sentence	denotes	There is a growing body of evidence suggesting that cAMP may function through both PKA-dependent and -independent mechanisms.6–8
T29	3641-3770	Sentence	denotes	LPS-induced activation of the transcription factor nuclear factor-κB (NF-κB) has also been the focus of a great deal of research.
T30	3771-3947	Sentence	denotes	It has been clearly demonstrated that agents that increase intracellular cAMP also inhibit NF-κB-dependent pro-inflammatory gene transcription, particularly of the TNF-α gene.9
T31	3948-4056	Sentence	denotes	Controversy exists regarding the exact mechanism(s) by which PDE inhibition down-regulates TNF-α production.
T32	4057-4322	Sentence	denotes	Possibilities include, but are not limited to, inhibition of NF-κB DNA binding activity, downregulation of NF-κB transcriptional activity, increased CREB activation, and competition between NF-κB and CREB for common co-activators such as CREB binding protein (CBP).
T33	4323-4407	Sentence	denotes	It is also not known whether these processes rely solely upon the activation of PKA.
T34	4408-4640	Sentence	denotes	Therefore, the objective of the present study is to determine the effects of nonspecific PDE inhibition with 1-[5-oxohexyl]-3,7-dimethylxanthine (Pentoxifylline; PTX) on NF-κB and CREB activation in vitro in human mononuclear cells.
T35	4641-4747	Sentence	denotes	With the use of specific inhibitors, we also investigated the role of PKA in LPS-induced TNF-α production.
T36	4749-4770	Sentence	denotes	MATERIALS AND METHODS
T37	4771-4872	Sentence	denotes	This study was approved by the Human Research Protections Program and the Institutional Review Board.
T38	4873-4974	Sentence	denotes	Written consent to participate in the study was obtained from all volunteers prior to blood donation.
T39	4975-5051	Sentence	denotes	Heparinized Vacutainers were purchased from Becton Dickinson (San Jose, CA).

events-check-again

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T887	32-38	Regulation	denotes	effect		true
T888	122-149	Protein	denotes	tumor necrosis factor-alpha
T889	151-156	Protein	denotes	TNF-α
T890	158-168	Gene_expression	denotes	production
T891	251-261	Gene_expression	denotes	production
T892	265-270	Protein	denotes	TNF-α
T893	271-280	Positive_regulation	denotes	following
T894	411-417	Regulation	denotes	alters
T895	418-423	Protein	denotes	TNF-α
T896	424-434	Gene_expression	denotes	production
T897	435-453	Positive_regulation	denotes	in the presence of
T898	667-681	Phosphorylation	denotes	phosphorylated
T899	694-699	Protein	denotes	I-κBα
T900	719-722	Protein	denotes	p65
T901	888-893	Protein	denotes	TNF-α
T902	1108-1114	Negative_regulation	denotes	reduce
T903	1108-1114	Negative_regulation	denotes	reduce
T904	1127-1132	Protein	denotes	I-κBα
T905	1133-1148	Phosphorylation	denotes	phosphorylation
T906	1158-1161	Protein	denotes	p65
T907	1162-1177	Phosphorylation	denotes	phosphorylation
T908	1352-1360	Localization	denotes	secreted
T909	1379-1384	Protein	denotes	TNF-α
T910	1392-1400	Regulation	denotes	presence
T911	1405-1412	Regulation	denotes	absence	true
T2369	2386-2395	Regulation	denotes	regulator
T2370	2399-2422	Protein	denotes	tumor necrosis factor-α
T2371	2424-2429	Protein	denotes	TNF-α
T2372	2431-2441	Gene_expression	denotes	production
T2373	2444-2449	Protein	denotes	TNF-α
T2374	2552-2557	Protein	denotes	TNF-α
T2375	2558-2567	Gene_expression	denotes	synthesis
T2376	2575-2585	Negative_regulation	denotes	attenuated
T2377	2780-2784	Positive_regulation	denotes	lead
T2378	2792-2806	Negative_regulation	denotes	downregulation
T2379	2810-2815	Protein	denotes	TNF-α
T2380	2816-2826	Gene_expression	denotes	production
T2381	3854-3861	Negative_regulation	denotes	inhibit
T2382	3868-3877	Regulation	denotes	dependent
T2383	3900-3913	Transcription	denotes	transcription
T2384	3935-3940	Protein	denotes	TNF-α
T2385	4024-4038	Negative_regulation	denotes	down-regulates
T2386	4039-4044	Protein	denotes	TNF-α
T2387	4045-4055	Gene_expression	denotes	production
T2388	4703-4707	Regulation	denotes	role		true
T2389	4722-4729	Positive_regulation	denotes	induced
T2390	4730-4735	Protein	denotes	TNF-α
T2391	4736-4746	Gene_expression	denotes	production
R650	T888	T890	themeOf	tumor necrosis factor-alpha,production
R651	T890	T887	themeOf	production,effect
R652	T891	T893	themeOf	production,following
R653	T892	T891	themeOf	TNF-α,production
R654	T894	T897	themeOf	alters,in the presence of
R655	T895	T896	themeOf	TNF-α,production
R656	T896	T894	themeOf	production,alters
R657	T899	T898	themeOf	I-κBα,phosphorylated
R658	T904	T905	themeOf	I-κBα,phosphorylation
R659	T905	T903	themeOf	phosphorylation,reduce
R660	T906	T907	themeOf	p65,phosphorylation
R661	T907	T902	themeOf	phosphorylation,reduce
R662	T908	T910	themeOf	secreted,presence
R663	T908	T911	themeOf	secreted,absence
R664	T909	T908	themeOf	TNF-α,secreted
R1831	T2370	T2372	themeOf	tumor necrosis factor-α,production
R1832	T2371	T2370	equivalentTo	TNF-α,tumor necrosis factor-α
R1833	T2372	T2369	themeOf	production,regulator
R1834	T2374	T2375	themeOf	TNF-α,synthesis
R1836	T2375	T2376	themeOf	synthesis,attenuated
R1838	T2378	T2377	themeOf	downregulation,lead
R1839	T2379	T2380	themeOf	TNF-α,production
R1840	T2380	T2378	themeOf	production,downregulation
R1842	T2382	T2381	themeOf	dependent,inhibit
R1843	T2383	T2382	themeOf	transcription,dependent
R1845	T2384	T2383	themeOf	TNF-α,transcription
R1846	T2386	T2387	themeOf	TNF-α,production
R1848	T2387	T2385	themeOf	production,down-regulates
R1849	T2389	T2388	themeOf	induced,role
R1850	T2390	T2391	themeOf	TNF-α,production
R1853	T2391	T2389	themeOf	production,induced

bionlp-st-ge-2016-reference-tees

Id	Subject	Object	Predicate	Lexical cue
T919	122-149	Protein	denotes	tumor necrosis factor-alpha
T920	151-156	Protein	denotes	TNF-α
T921	158-168	Gene_expression	denotes	production
T922	158-168	Gene_expression	denotes	production
T923	107-117	Positive_regulation	denotes	activation
T924	107-117	Positive_regulation	denotes	activation
T925	32-38	Regulation	denotes	effect
T926	32-38	Regulation	denotes	effect
T927	265-270	Protein	denotes	TNF-α
T928	251-261	Gene_expression	denotes	production
T929	285-296	Positive_regulation	denotes	stimulation
T930	418-423	Protein	denotes	TNF-α
T931	424-434	Gene_expression	denotes	production
T932	411-417	Regulation	denotes	alters
T933	667-699	Protein	denotes	phosphorylated cytoplasmic I-κBα
T934	701-718	Protein	denotes	nuclear factor-κB
T935	719-722	Protein	denotes	p65
T936	724-729	Protein	denotes	NF-κB
T937	736-781	Protein	denotes	nuclear cAMP-response element binding protein
T938	783-787	Protein	denotes	CREB
T939	819-824	Protein	denotes	NF-κB
T940	829-833	Protein	denotes	CREB
T941	808-815	Binding	denotes	binding
T942	808-815	Binding	denotes	binding
T943	888-893	Protein	denotes	TNF-α
T944	984-1000	Protein	denotes	Protein kinase A
T945	1001-1011	Negative_regulation	denotes	inhibition
T946	1070-1073	Protein	denotes	PTX
T947	1115-1128	Protein	denotes	cytoplasmic I
T948	1129-1132	Protein	denotes	κBα
T949	1158-1161	Protein	denotes	p65
T950	1211-1216	Protein	denotes	NF-κB
T951	1133-1148	Phosphorylation	denotes	phosphorylation
T952	1133-1148	Phosphorylation	denotes	phosphorylation
T953	1150-1157	Entity	denotes	nuclear
T954	1162-1177	Phosphorylation	denotes	phosphorylation
T955	1191-1198	Binding	denotes	binding
T956	1108-1114	Negative_regulation	denotes	reduce
T957	1231-1234	Protein	denotes	PTX
T958	1301-1305	Protein	denotes	CREB
T959	1257-1272	Phosphorylation	denotes	phosphorylation
T960	1244-1252	Positive_regulation	denotes	enhanced
T961	1340-1343	Protein	denotes	PTX
T962	1379-1384	Protein	denotes	TNF-α
T963	1413-1429	Protein	denotes	Protein kinase A
T964	1352-1360	Localization	denotes	secreted
T965	1352-1360	Localization	denotes	secreted
T966	1430-1440	Negative_regulation	denotes	inhibition
T967	1601-1606	Protein	denotes	NF-κB
T968	1629-1633	Protein	denotes	CREB
T969	1654-1661	Binding	denotes	binding
T970	1615-1625	Positive_regulation	denotes	activation
T971	1740-1743	Protein	denotes	PTX
T972	1809-1825	Protein	denotes	Protein kinase A
T2232	2057-2126	Protein	denotes	multiple host intracellular protein kinases and transcription factors
T2233	2023-2038	Phosphorylation	denotes	phosphorylation
T2234	2043-2053	Positive_regulation	denotes	activation
T2235	2012-2022	Positive_regulation	denotes	initiating
T2236	2012-2022	Positive_regulation	denotes	initiating
T2237	2399-2422	Protein	denotes	tumor necrosis factor-α
T2238	2424-2429	Protein	denotes	TNF-α
T2239	2444-2449	Protein	denotes	TNF-α
T2240	2386-2395	Regulation	denotes	regulator
T2241	2386-2395	Regulation	denotes	regulator
T2242	2552-2557	Protein	denotes	TNF-α
T2243	2632-2635	Protein	denotes	PDE
T2244	2810-2815	Protein	denotes	TNF-α
T2245	2613-2630	Protein	denotes	phosphodiesterase
T2246	2558-2567	Gene_expression	denotes	synthesis
T2247	2575-2585	Negative_regulation	denotes	attenuated
T2248	2637-2647	Negative_regulation	denotes	inhibition
T2249	2637-2647	Negative_regulation	denotes	inhibition
T2250	2816-2826	Gene_expression	denotes	production
T2251	2792-2806	Negative_regulation	denotes	downregulation
T2252	3079-3095	Protein	denotes	Protein kinase A
T2253	3097-3100	Protein	denotes	PKA
T2254	3104-3107	Protein	denotes	PKA
T2255	3181-3218	Protein	denotes	cAMP-response element binding protein
T2256	3220-3224	Protein	denotes	CREB
T2257	2971-2983	Positive_regulation	denotes	accumulation
T2258	3065-3075	Positive_regulation	denotes	activation
T2259	3108-3118	Positive_regulation	denotes	activation
T2260	3108-3118	Positive_regulation	denotes	activation
T2261	3124-3129	Positive_regulation	denotes	leads
T2262	3124-3129	Positive_regulation	denotes	leads
T2263	3137-3152	Phosphorylation	denotes	phosphorylation
T2264	3137-3152	Phosphorylation	denotes	phosphorylation
T2265	2942-2949	Positive_regulation	denotes	results
T2266	3595-3598	Protein	denotes	PKA
T2267	3671-3709	Protein	denotes	transcription factor nuclear factor-κB
T2268	3711-3716	Protein	denotes	NF-κB
T2269	3653-3663	Positive_regulation	denotes	activation
T2270	3653-3663	Positive_regulation	denotes	activation
T2271	3862-3867	Protein	denotes	NF-κB
T2272	3935-3940	Protein	denotes	TNF-α
T2273	3900-3913	Transcription	denotes	transcription
T2274	3900-3913	Transcription	denotes	transcription
T2275	3854-3861	Negative_regulation	denotes	inhibit
T2276	3854-3861	Negative_regulation	denotes	inhibit
T2277	4009-4012	Protein	denotes	PDE
T2278	4039-4044	Protein	denotes	TNF-α
T2279	4013-4023	Negative_regulation	denotes	inhibition
T2280	4045-4055	Gene_expression	denotes	production
T2281	4024-4038	Negative_regulation	denotes	down-regulates
T2282	4118-4123	Protein	denotes	NF-κB
T2283	4164-4169	Protein	denotes	NF-κB
T2284	4206-4210	Protein	denotes	CREB
T2285	4247-4252	Protein	denotes	NF-κB
T2286	4257-4261	Protein	denotes	CREB
T2287	4295-4315	Protein	denotes	CREB binding protein
T2288	4317-4320	Protein	denotes	CBP
T2289	4128-4135	Binding	denotes	binding
T2290	4211-4221	Positive_regulation	denotes	activation
T2291	4227-4238	Binding	denotes	competition
T2292	4104-4114	Negative_regulation	denotes	inhibition
T2293	4403-4406	Protein	denotes	PKA
T2294	4389-4399	Positive_regulation	denotes	activation
T2295	4578-4583	Protein	denotes	NF-κB
T2296	4588-4592	Protein	denotes	CREB
T2297	4593-4603	Positive_regulation	denotes	activation
T2298	4593-4603	Positive_regulation	denotes	activation
T2299	4711-4714	Protein	denotes	PKA
T2300	4730-4735	Protein	denotes	TNF-α
T2301	4736-4746	Gene_expression	denotes	production
T2302	4703-4707	Positive_regulation	denotes	role
T2303	4718-4729	Positive_regulation	denotes	LPS-induced
R669	T919	T921	themeOf	tumor necrosis factor-alpha,production
R670	T920	T922	themeOf	TNF-α,production
R671	T921	T923	themeOf	production,activation
R672	T922	T924	themeOf	production,activation
R673	T923	T925	themeOf	activation,effect
R674	T924	T926	themeOf	activation,effect
R675	T927	T928	themeOf	TNF-α,production
R676	T928	T929	themeOf	production,stimulation
R677	T930	T931	themeOf	TNF-α,production
R678	T931	T932	themeOf	production,alters
R679	T939	T941	themeOf	NF-κB,binding
R680	T940	T942	themeOf	CREB,binding
R681	T944	T945	themeOf	Protein kinase A,inhibition
R682	T946	T956	causeOf	PTX,reduce
R683	T947	T951	themeOf	cytoplasmic I,phosphorylation
R684	T948	T952	themeOf	κBα,phosphorylation
R685	T949	T954	themeOf	p65,phosphorylation
R686	T950	T955	themeOf	NF-κB,binding
R687	T953	T954	Site	nuclear,phosphorylation
R688	T954	T956	themeOf	phosphorylation,reduce
R689	T957	T960	causeOf	PTX,enhanced
R690	T958	T959	themeOf	CREB,phosphorylation
R691	T959	T960	themeOf	phosphorylation,enhanced
R692	T961	T964	themeOf	PTX,secreted
R693	T962	T965	themeOf	TNF-α,secreted
R694	T963	T966	themeOf	Protein kinase A,inhibition
R695	T967	T969	themeOf	NF-κB,binding
R696	T969	T970	themeOf	binding,activation
R1748	T2232	T2233	themeOf	multiple host intracellular protein kinases and transcription factors,phosphorylation
R1749	T2233	T2234	themeOf	phosphorylation,activation
R1750	T2233	T2235	themeOf	phosphorylation,initiating
R1751	T2234	T2236	themeOf	activation,initiating
R1752	T2237	T2240	themeOf	tumor necrosis factor-α,regulator
R1753	T2238	T2241	themeOf	TNF-α,regulator
R1754	T2242	T2246	themeOf	TNF-α,synthesis
R1755	T2243	T2248	themeOf	PDE,inhibition
R1756	T2244	T2250	themeOf	TNF-α,production
R1757	T2245	T2249	themeOf	phosphodiesterase,inhibition
R1758	T2246	T2247	themeOf	synthesis,attenuated
R1759	T2250	T2251	themeOf	production,downregulation
R1760	T2280	T2281	themeOf	production,down-regulates
R1761	T2252	T2257	themeOf	Protein kinase A,accumulation
R1762	T2282	T2289	themeOf	NF-κB,binding
R1763	T2253	T2259	themeOf	PKA,activation
R1764	T2284	T2290	themeOf	CREB,activation
R1765	T2285	T2291	themeOf	NF-κB,competition
R1766	T2286	T2291	themeOf	CREB,competition
R1767	T2289	T2292	themeOf	binding,inhibition
R1768	T2254	T2260	themeOf	PKA,activation
R1769	T2255	T2263	themeOf	cAMP-response element binding protein,phosphorylation
R1770	T2293	T2294	themeOf	PKA,activation
R1771	T2256	T2264	themeOf	CREB,phosphorylation
R1772	T2295	T2297	themeOf	NF-κB,activation
R1773	T2296	T2298	themeOf	CREB,activation
R1774	T2257	T2258	themeOf	accumulation,activation
R1775	T2258	T2265	themeOf	activation,results
R1776	T2299	T2302	causeOf	PKA,role
R1777	T2300	T2301	themeOf	TNF-α,production
R1778	T2301	T2302	themeOf	production,role
R1779	T2301	T2303	themeOf	production,LPS-induced
R1780	T2259	T2261	themeOf	activation,leads
R1781	T2260	T2262	themeOf	activation,leads
R1782	T2267	T2269	themeOf	transcription factor nuclear factor-κB,activation
R1783	T2268	T2270	themeOf	NF-κB,activation
R1784	T2271	T2273	themeOf	NF-κB,transcription
R1785	T2272	T2274	themeOf	TNF-α,transcription
R1787	T2272	T2275	causeOf	TNF-α,inhibit
R1788	T2272	T2276	causeOf	TNF-α,inhibit
R1789	T2273	T2275	themeOf	transcription,inhibit
R1791	T2274	T2276	themeOf	transcription,inhibit
R1792	T2277	T2279	themeOf	PDE,inhibition
R1794	T2278	T2280	themeOf	TNF-α,production
R1796	T2279	T2281	causeOf	inhibition,down-regulates

bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T759	122-143	P01375	denotes	tumor necrosis factor
T760	151-154	P01375	denotes	TNF
T761	151-156	P01375	denotes	TNF-α
T762	265-268	P01375	denotes	TNF
T763	265-270	P01375	denotes	TNF-α
T764	418-421	P01375	denotes	TNF
T765	418-423	P01375	denotes	TNF-α
T766	719-722	P21579	denotes	p65
T767	719-722	Q04206	denotes	p65
T768	744-781	O43889	denotes	cAMP-response element binding protein
T769	744-781	P15336	denotes	cAMP-response element binding protein
T770	744-781	P18848	denotes	cAMP-response element binding protein
T771	744-781	Q8TEY5	denotes	cAMP-response element binding protein
T772	744-781	Q02930	denotes	cAMP-response element binding protein
T773	744-781	P16220	denotes	cAMP-response element binding protein
T774	783-787	P16220	denotes	CREB
T775	783-787	O43889	denotes	CREB
T776	783-787	Q8TEY5	denotes	CREB
T777	783-787	Q02930	denotes	CREB
T778	783-787	P18848	denotes	CREB
T779	783-787	P15336	denotes	CREB
T780	829-833	O43889	denotes	CREB
T781	829-833	Q02930	denotes	CREB
T782	829-833	P15336	denotes	CREB
T783	829-833	P16220	denotes	CREB
T784	829-833	Q8TEY5	denotes	CREB
T785	829-833	P18848	denotes	CREB
T786	888-891	P01375	denotes	TNF
T787	888-893	P01375	denotes	TNF-α
T788	1158-1161	Q04206	denotes	p65
T789	1158-1161	P21579	denotes	p65
T790	1301-1305	O43889	denotes	CREB
T791	1301-1305	Q8TEY5	denotes	CREB
T792	1301-1305	Q02930	denotes	CREB
T793	1301-1305	P18848	denotes	CREB
T794	1301-1305	P16220	denotes	CREB
T795	1301-1305	P15336	denotes	CREB
T796	1379-1382	P01375	denotes	TNF
T797	1379-1384	P01375	denotes	TNF-α
T798	1629-1633	P16220	denotes	CREB
T799	1629-1633	P18848	denotes	CREB
T800	1629-1633	Q02930	denotes	CREB
T801	1629-1633	P15336	denotes	CREB
T802	1629-1633	Q8TEY5	denotes	CREB
T803	1629-1633	O43889	denotes	CREB
T2041	2105-2148	Q02548	denotes	transcription factors. Transcription factor
T2042	2105-2148	Q9UL17	denotes	transcription factors. Transcription factor
T2043	2399-2420	P01375	denotes	tumor necrosis factor
T2044	2424-2427	P01375	denotes	TNF
T2045	2424-2429	P01375	denotes	TNF-α
T2046	2444-2447	P01375	denotes	TNF
T2047	2444-2449	P01375	denotes	TNF-α
T2048	2552-2555	P01375	denotes	TNF
T2049	2552-2557	P01375	denotes	TNF-α
T2050	2810-2813	P01375	denotes	TNF
T2051	2810-2815	P01375	denotes	TNF-α
T2052	3181-3218	P15336	denotes	cAMP-response element binding protein
T2053	3181-3218	Q02930	denotes	cAMP-response element binding protein
T2054	3181-3218	P16220	denotes	cAMP-response element binding protein
T2055	3181-3218	O43889	denotes	cAMP-response element binding protein
T2056	3181-3218	P18848	denotes	cAMP-response element binding protein
T2057	3181-3218	Q8TEY5	denotes	cAMP-response element binding protein
T2058	3220-3224	Q8TEY5	denotes	CREB
T2059	3220-3224	P15336	denotes	CREB
T2060	3220-3224	P16220	denotes	CREB
T2061	3220-3224	P18848	denotes	CREB
T2062	3220-3224	Q02930	denotes	CREB
T2063	3220-3224	O43889	denotes	CREB
T2064	3935-3938	P01375	denotes	TNF
T2065	3935-3940	P01375	denotes	TNF-α
T2066	4039-4042	P01375	denotes	TNF
T2067	4039-4044	P01375	denotes	TNF-α
T2068	4206-4210	O43889	denotes	CREB
T2069	4206-4210	P15336	denotes	CREB
T2070	4206-4210	P16220	denotes	CREB
T2071	4206-4210	Q8TEY5	denotes	CREB
T2072	4206-4210	P18848	denotes	CREB
T2073	4206-4210	Q02930	denotes	CREB
T2074	4257-4261	O43889	denotes	CREB
T2075	4257-4261	Q8TEY5	denotes	CREB
T2076	4257-4261	P15336	denotes	CREB
T2077	4257-4261	P16220	denotes	CREB
T2078	4257-4261	P18848	denotes	CREB
T2079	4257-4261	Q02930	denotes	CREB
T2080	4295-4299	P16220	denotes	CREB
T2081	4295-4299	P15336	denotes	CREB
T2082	4295-4299	P18848	denotes	CREB
T2083	4295-4299	Q8TEY5	denotes	CREB
T2084	4295-4299	O43889	denotes	CREB
T2085	4295-4299	Q02930	denotes	CREB
T2086	4295-4315	Q92793	denotes	CREB binding protein
T2087	4317-4320	Q92793	denotes	CBP
T2088	4588-4592	P15336	denotes	CREB
T2089	4588-4592	Q02930	denotes	CREB
T2090	4588-4592	P16220	denotes	CREB
T2091	4588-4592	Q8TEY5	denotes	CREB
T2092	4588-4592	P18848	denotes	CREB
T2093	4588-4592	O43889	denotes	CREB
T2094	4730-4733	P01375	denotes	TNF
T2095	4730-4735	P01375	denotes	TNF-α
T2038	2101-2148	Q9NY61	denotes	and transcription factors. Transcription factor
T2039	2105-2141	Q99853	denotes	transcription factors. Transcription
T2040	2105-2141	Q9UL17	denotes	transcription factors. Transcription

test2

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T32	122-149	Protein	denotes	tumor necrosis factor-alpha
T33	151-156	Protein	denotes	TNF-α
T34	158-168	Gene_expression	denotes	production
T35	251-261	Gene_expression	denotes	production
T36	265-270	Protein	denotes	TNF-α
T37	271-280	Positive_regulation	denotes	following
T38	411-417	Regulation	denotes	alters
T39	418-423	Protein	denotes	TNF-α
T40	424-434	Gene_expression	denotes	production
T41	667-681	Phosphorylation	denotes	phosphorylated
T42	694-699	Protein	denotes	I-κBα
T43	719-722	Protein	denotes	p65
T44	888-893	Protein	denotes	TNF-α
T45	1108-1114	Negative_regulation	denotes	reduce	true
T46	1127-1132	Protein	denotes	I-κBα
T47	1133-1148	Phosphorylation	denotes	phosphorylation
T48	1158-1161	Protein	denotes	p65
T49	1162-1177	Phosphorylation	denotes	phosphorylation
T50	1191-1198	Binding	denotes	binding
T51	1244-1252	Positive_regulation	denotes	enhanced
T52	1352-1360	Gene_expression	denotes	secreted
T53	1379-1384	Protein	denotes	TNF-α
T1103	2386-2395	Regulation	denotes	regulator
T1104	2399-2422	Protein	denotes	tumor necrosis factor-α
T1105	2424-2429	Protein	denotes	TNF-α
T1106	2431-2441	Gene_expression	denotes	production
T1107	2444-2449	Protein	denotes	TNF-α
T1108	2552-2557	Protein	denotes	TNF-α
T1109	2558-2567	Gene_expression	denotes	synthesis
T1110	2575-2585	Negative_regulation	denotes	attenuated
T1111	2792-2806	Negative_regulation	denotes	downregulation
T1112	2810-2815	Protein	denotes	TNF-α
T1113	2816-2826	Gene_expression	denotes	production
T1114	3854-3861	Negative_regulation	denotes	inhibit
T1115	3868-3877	Regulation	denotes	dependent		true
T1116	3935-3940	Protein	denotes	TNF-α
T1117	4013-4023	Negative_regulation	denotes	inhibition
T1118	4024-4038	Negative_regulation	denotes	down-regulates
T1119	4039-4044	Protein	denotes	TNF-α
T1120	4045-4055	Gene_expression	denotes	production
T1121	4703-4707	Regulation	denotes	role
T1122	4722-4729	Positive_regulation	denotes	induced
T1123	4730-4735	Protein	denotes	TNF-α
T1124	4736-4746	Gene_expression	denotes	production
R20	T32	T34	themeOf	tumor necrosis factor-alpha,production
R21	T35	T37	themeOf	production,following
R22	T36	T35	themeOf	TNF-α,production
R23	T39	T40	themeOf	TNF-α,production
R24	T40	T38	themeOf	production,alters
R25	T42	T41	themeOf	I-κBα,phosphorylated
R26	T46	T47	themeOf	I-κBα,phosphorylation
R27	T47	T45	themeOf	phosphorylation,reduce
R28	T48	T49	themeOf	p65,phosphorylation
R29	T48	T50	themeOf	p65,binding
R30	T49	T45	themeOf	phosphorylation,reduce
R31	T50	T45	themeOf	binding,reduce
R32	T53	T52	themeOf	TNF-α,secreted
R820	T1104	T1106	themeOf	tumor necrosis factor-α,production
R821	T1105	T1104	equivalentTo	TNF-α,tumor necrosis factor-α
R822	T1106	T1103	themeOf	production,regulator
R823	T1108	T1109	themeOf	TNF-α,synthesis
R824	T1109	T1110	themeOf	synthesis,attenuated
R825	T1112	T1113	themeOf	TNF-α,production
R826	T1113	T1111	themeOf	production,downregulation
R827	T1115	T1114	themeOf	dependent,inhibit
R828	T1119	T1120	themeOf	TNF-α,production
R829	T1120	T1118	themeOf	production,down-regulates
R830	T1123	T1124	themeOf	TNF-α,production
R831	T1124	T1122	themeOf	production,induced

PMC:2664230 / 172-5223 JSONTXT

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PMC:2664230 / 172-5223 JSON TXT