PMC:2664230 / 14139-15240 JSONTXT

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T6483","span":{"begin":255,"end":264},"obj":"Positive_regulation"},{"id":"T6484","span":{"begin":265,"end":274},"obj":"Positive_regulation"},{"id":"T6485","span":{"begin":383,"end":396},"obj":"Negative_regulation"},{"id":"T6486","span":{"begin":401,"end":408},"obj":"Positive_regulation"},{"id":"T6487","span":{"begin":409,"end":414},"obj":"Protein"},{"id":"T6488","span":{"begin":415,"end":430},"obj":"Phosphorylation"},{"id":"T6489","span":{"begin":488,"end":491},"obj":"Protein"},{"id":"T6490","span":{"begin":492,"end":507},"obj":"Phosphorylation"},{"id":"T6491","span":{"begin":512,"end":521},"obj":"Positive_regulation"},{"id":"T6492","span":{"begin":522,"end":531},"obj":"Positive_regulation"},{"id":"T6493","span":{"begin":595,"end":604},"obj":"Negative_regulation"},{"id":"T6494","span":{"begin":609,"end":616},"obj":"Positive_regulation"},{"id":"T6495","span":{"begin":623,"end":626},"obj":"Protein"},{"id":"T6496","span":{"begin":635,"end":650},"obj":"Phosphorylation"},{"id":"T6476","span":{"begin":65,"end":70},"obj":"Positive_regulation"},{"id":"T6477","span":{"begin":93,"end":104},"obj":"Protein_catabolism"},{"id":"T6478","span":{"begin":108,"end":113},"obj":"Protein"},{"id":"T6479","span":{"begin":183,"end":190},"obj":"Regulation"},{"id":"T6480","span":{"begin":201,"end":206},"obj":"Protein"},{"id":"T6481","span":{"begin":220,"end":225},"obj":"Protein"},{"id":"T6482","span":{"begin":226,"end":241},"obj":"Phosphorylation"}],"relations":[{"id":"R5365","pred":"themeOf","subj":"T6477","obj":"T6476"},{"id":"R5367","pred":"themeOf","subj":"T6480","obj":"T6479"},{"id":"R5368","pred":"themeOf","subj":"T6481","obj":"T6482"},{"id":"R5369","pred":"themeOf","subj":"T6482","obj":"T6483"},{"id":"R5370","pred":"themeOf","subj":"T6483","obj":"T6484"},{"id":"R5371","pred":"themeOf","subj":"T6486","obj":"T6485"},{"id":"R5372","pred":"themeOf","subj":"T6487","obj":"T6488"},{"id":"R5373","pred":"themeOf","subj":"T6488","obj":"T6486"},{"id":"R5374","pred":"themeOf","subj":"T6489","obj":"T6490"},{"id":"R5375","pred":"themeOf","subj":"T6490","obj":"T6491"},{"id":"R5376","pred":"themeOf","subj":"T6491","obj":"T6492"},{"id":"R5377","pred":"themeOf","subj":"T6494","obj":"T6493"},{"id":"R5378","pred":"themeOf","subj":"T6495","obj":"T6496"},{"id":"R5379","pred":"themeOf","subj":"T6496","obj":"T6494"},{"id":"R5366","pred":"themeOf","subj":"T6478","obj":"T6477"}],"attributes":[{"id":"M35","pred":"Speculation","subj":"T6479","obj":"true"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

{"project":"pmc-enju-pas","denotations":[{"id":"T6696","span":{"begin":0,"end":3},"obj":"NN"},{"id":"T6697","span":{"begin":4,"end":13},"obj":"VB"},{"id":"T6698","span":{"begin":14,"end":25},"obj":"JJ"},{"id":"T6699","span":{"begin":26,"end":31},"obj":"NN"},{"id":"T6700","span":{"begin":32,"end":42},"obj":"NN"},{"id":"T6701","span":{"begin":43,"end":48},"obj":"IN"},{"id":"T6702","span":{"begin":49,"end":64},"obj":"NN"},{"id":"T6703","span":{"begin":65,"end":70},"obj":"VB"},{"id":"T6704","span":{"begin":71,"end":73},"obj":"TO"},{"id":"T6705","span":{"begin":74,"end":88},"obj":"NN"},{"id":"T6706","span":{"begin":89,"end":92},"obj":"CC"},{"id":"T6707","span":{"begin":93,"end":104},"obj":"NN"},{"id":"T6708","span":{"begin":105,"end":107},"obj":"IN"},{"id":"T6709","span":{"begin":108,"end":113},"obj":"JJ"},{"id":"T6710","span":{"begin":114,"end":117},"obj":"CC"},{"id":"T6711","span":{"begin":118,"end":128},"obj":"JJ"},{"id":"T6712","span":{"begin":129,"end":136},"obj":"JJ"},{"id":"T6713","span":{"begin":137,"end":150},"obj":"NN"},{"id":"T6714","span":{"begin":151,"end":153},"obj":"IN"},{"id":"T6715","span":{"begin":154,"end":159},"obj":"NN"},{"id":"T6716","span":{"begin":159,"end":160},"obj":"-COMMA-"},{"id":"T6717","span":{"begin":161,"end":163},"obj":"PRP"},{"id":"T6718","span":{"begin":164,"end":169},"obj":"RB"},{"id":"T6719","span":{"begin":170,"end":178},"obj":"VB"},{"id":"T6720","span":{"begin":179,"end":182},"obj":"DT"},{"id":"T6721","span":{"begin":183,"end":190},"obj":"NN"},{"id":"T6722","span":{"begin":191,"end":193},"obj":"IN"},{"id":"T6723","span":{"begin":194,"end":197},"obj":"NN"},{"id":"T6724","span":{"begin":198,"end":200},"obj":"IN"},{"id":"T6725","span":{"begin":201,"end":206},"obj":"NN"},{"id":"T6726","span":{"begin":208,"end":219},"obj":"JJ"},{"id":"T6727","span":{"begin":220,"end":225},"obj":"JJ"},{"id":"T6728","span":{"begin":226,"end":241},"obj":"NN"},{"id":"T6729","span":{"begin":242,"end":245},"obj":"VB"},{"id":"T6730","span":{"begin":246,"end":254},"obj":"RB"},{"id":"T6731","span":{"begin":255,"end":264},"obj":"VB"},{"id":"T6732","span":{"begin":265,"end":274},"obj":"VB"},{"id":"T6733","span":{"begin":275,"end":278},"obj":"NN"},{"id":"T6734","span":{"begin":279,"end":290},"obj":"NN"},{"id":"T6735","span":{"begin":291,"end":295},"obj":"WRB"},{"id":"T6736","span":{"begin":296,"end":304},"obj":"VB"},{"id":"T6737","span":{"begin":305,"end":307},"obj":"TO"},{"id":"T6738","span":{"begin":308,"end":315},"obj":"NN"},{"id":"T6739","span":{"begin":316,"end":317},"obj":"-LRB-"},{"id":"T6740","span":{"begin":317,"end":320},"obj":"CD"},{"id":"T6741","span":{"begin":321,"end":322},"obj":"SYM"},{"id":"T6742","span":{"begin":323,"end":324},"obj":"CD"},{"id":"T6743","span":{"begin":325,"end":328},"obj":"IN"},{"id":"T6744","span":{"begin":329,"end":331},"obj":"CD"},{"id":"T6745","span":{"begin":332,"end":333},"obj":"CC"},{"id":"T6746","span":{"begin":334,"end":336},"obj":"CD"},{"id":"T6747","span":{"begin":336,"end":337},"obj":"-COLON-"},{"id":"T6748","span":{"begin":338,"end":339},"obj":"NN"},{"id":"T6749","span":{"begin":340,"end":341},"obj":"SYM"},{"id":"T6750","span":{"begin":342,"end":346},"obj":"CD"},{"id":"T6751","span":{"begin":346,"end":347},"obj":"-RRB-"},{"id":"T6752","span":{"begin":349,"end":352},"obj":"DT"},{"id":"T6753","span":{"begin":353,"end":361},"obj":"NN"},{"id":"T6754","span":{"begin":362,"end":364},"obj":"IN"},{"id":"T6755","span":{"begin":365,"end":368},"obj":"NN"},{"id":"T6756","span":{"begin":369,"end":382},"obj":"RB"},{"id":"T6757","span":{"begin":383,"end":396},"obj":"VB"},{"id":"T6758","span":{"begin":397,"end":408},"obj":"JJ"},{"id":"T6759","span":{"begin":409,"end":414},"obj":"NN"},{"id":"T6760","span":{"begin":415,"end":430},"obj":"NN"},{"id":"T6761","span":{"begin":431,"end":432},"obj":"-LRB-"},{"id":"T6762","span":{"begin":432,"end":433},"obj":"NN"},{"id":"T6763","span":{"begin":434,"end":435},"obj":"SYM"},{"id":"T6764","span":{"begin":436,"end":440},"obj":"CD"},{"id":"T6765","span":{"begin":440,"end":441},"obj":"-COLON-"},{"id":"T6766","span":{"begin":442,"end":448},"obj":"NN"},{"id":"T6767","span":{"begin":449,"end":450},"obj":"CD"},{"id":"T6768","span":{"begin":450,"end":451},"obj":"-RRB-"},{"id":"T6769","span":{"begin":452,"end":454},"obj":"IN"},{"id":"T6770","span":{"begin":455,"end":456},"obj":"DT"},{"id":"T6771","span":{"begin":457,"end":464},"obj":"JJ"},{"id":"T6772","span":{"begin":465,"end":472},"obj":"NN"},{"id":"T6773","span":{"begin":472,"end":473},"obj":"-COMMA-"},{"id":"T6774","span":{"begin":474,"end":481},"obj":"JJ"},{"id":"T6775","span":{"begin":482,"end":487},"obj":"NN"},{"id":"T6776","span":{"begin":488,"end":491},"obj":"NN"},{"id":"T6777","span":{"begin":492,"end":507},"obj":"NN"},{"id":"T6778","span":{"begin":508,"end":511},"obj":"VB"},{"id":"T6779","span":{"begin":512,"end":521},"obj":"VB"},{"id":"T6780","span":{"begin":522,"end":531},"obj":"VB"},{"id":"T6781","span":{"begin":532,"end":535},"obj":"NN"},{"id":"T6782","span":{"begin":536,"end":547},"obj":"NN"},{"id":"T6783","span":{"begin":548,"end":549},"obj":"-LRB-"},{"id":"T6784","span":{"begin":549,"end":552},"obj":"CD"},{"id":"T6785","span":{"begin":553,"end":554},"obj":"SYM"},{"id":"T6786","span":{"begin":555,"end":556},"obj":"CD"},{"id":"T6787","span":{"begin":557,"end":560},"obj":"IN"},{"id":"T6788","span":{"begin":561,"end":563},"obj":"CD"},{"id":"T6789","span":{"begin":564,"end":565},"obj":"CC"},{"id":"T6790","span":{"begin":566,"end":568},"obj":"CD"},{"id":"T6791","span":{"begin":568,"end":569},"obj":"-COLON-"},{"id":"T6792","span":{"begin":570,"end":571},"obj":"NN"},{"id":"T6793","span":{"begin":572,"end":573},"obj":"SYM"},{"id":"T6794","span":{"begin":574,"end":578},"obj":"CD"},{"id":"T6795","span":{"begin":578,"end":579},"obj":"-RRB-"},{"id":"T6796","span":{"begin":581,"end":584},"obj":"NN"},{"id":"T6797","span":{"begin":585,"end":594},"obj":"RB"},{"id":"T6798","span":{"begin":595,"end":604},"obj":"VB"},{"id":"T6799","span":{"begin":605,"end":616},"obj":"JJ"},{"id":"T6800","span":{"begin":617,"end":622},"obj":"NN"},{"id":"T6801","span":{"begin":623,"end":626},"obj":"NN"},{"id":"T6802","span":{"begin":627,"end":634},"obj":"JJ"},{"id":"T6803","span":{"begin":635,"end":650},"obj":"NN"},{"id":"T6804","span":{"begin":650,"end":651},"obj":"-COMMA-"},{"id":"T6805","span":{"begin":652,"end":653},"obj":"DT"},{"id":"T6806","span":{"begin":654,"end":660},"obj":"NN"},{"id":"T6807","span":{"begin":661,"end":663},"obj":"IN"},{"id":"T6808","span":{"begin":664,"end":669},"obj":"NN"},{"id":"T6809","span":{"begin":670,"end":680},"obj":"NN"},{"id":"T6810","span":{"begin":681,"end":684},"obj":"CC"},{"id":"T6811","span":{"begin":685,"end":692},"obj":"JJ"},{"id":"T6812","span":{"begin":693,"end":706},"obj":"NN"},{"id":"T6813","span":{"begin":707,"end":708},"obj":"-LRB-"},{"id":"T6814","span":{"begin":708,"end":711},"obj":"CD"},{"id":"T6815","span":{"begin":712,"end":713},"obj":"SYM"},{"id":"T6816","span":{"begin":714,"end":715},"obj":"CD"},{"id":"T6817","span":{"begin":716,"end":719},"obj":"IN"},{"id":"T6818","span":{"begin":720,"end":722},"obj":"CD"},{"id":"T6819","span":{"begin":723,"end":724},"obj":"CC"},{"id":"T6820","span":{"begin":725,"end":726},"obj":"CD"},{"id":"T6821","span":{"begin":726,"end":727},"obj":"-COLON-"},{"id":"T6822","span":{"begin":728,"end":729},"obj":"NN"},{"id":"T6823","span":{"begin":730,"end":735},"obj":"CD"},{"id":"T6824","span":{"begin":735,"end":736},"obj":"-COLON-"},{"id":"T6825","span":{"begin":737,"end":743},"obj":"NN"},{"id":"T6826","span":{"begin":744,"end":746},"obj":"NN"},{"id":"T6827","span":{"begin":746,"end":747},"obj":"-RRB-"},{"id":"T6828","span":{"begin":749,"end":754},"obj":"NN"},{"id":"T6829","span":{"begin":755,"end":759},"obj":"VB"},{"id":"T6830","span":{"begin":760,"end":764},"obj":"RB"},{"id":"T6831","span":{"begin":765,"end":774},"obj":"VB"},{"id":"T6832","span":{"begin":775,"end":777},"obj":"TO"},{"id":"T6833","span":{"begin":778,"end":784},"obj":"VB"},{"id":"T6834","span":{"begin":785,"end":788},"obj":"DT"},{"id":"T6835","span":{"begin":789,"end":800},"obj":"JJ"},{"id":"T6836","span":{"begin":801,"end":812},"obj":"NN"},{"id":"T6837","span":{"begin":813,"end":815},"obj":"IN"},{"id":"T6838","span":{"begin":816,"end":823},"obj":"JJ"},{"id":"T6839","span":{"begin":824,"end":839},"obj":"NN"},{"id":"T6840","span":{"begin":840,"end":848},"obj":"VB"},{"id":"T6841","span":{"begin":849,"end":851},"obj":"IN"},{"id":"T6842","span":{"begin":852,"end":859},"obj":"JJ"},{"id":"T6843","span":{"begin":860,"end":867},"obj":"NN"},{"id":"T6844","span":{"begin":868,"end":870},"obj":"IN"},{"id":"T6845","span":{"begin":871,"end":874},"obj":"DT"},{"id":"T6846","span":{"begin":875,"end":878},"obj":"NN"},{"id":"T6847","span":{"begin":879,"end":886},"obj":"NN"},{"id":"T6848","span":{"begin":887,"end":895},"obj":"NN"},{"id":"T6849","span":{"begin":896,"end":898},"obj":"IN"},{"id":"T6850","span":{"begin":899,"end":904},"obj":"NN"},{"id":"T6851","span":{"begin":906,"end":909},"obj":"DT"},{"id":"T6852","span":{"begin":910,"end":918},"obj":"NN"},{"id":"T6853","span":{"begin":919,"end":921},"obj":"IN"},{"id":"T6854","span":{"begin":922,"end":925},"obj":"NN"},{"id":"T6855","span":{"begin":926,"end":928},"obj":"TO"},{"id":"T6856","span":{"begin":929,"end":943},"obj":"JJ"},{"id":"T6857","span":{"begin":944,"end":955},"obj":"JJ"},{"id":"T6858","span":{"begin":956,"end":961},"obj":"NN"},{"id":"T6859","span":{"begin":962,"end":970},"obj":"VB"},{"id":"T6860","span":{"begin":971,"end":973},"obj":"IN"},{"id":"T6861","span":{"begin":974,"end":984},"obj":"JJ"},{"id":"T6862","span":{"begin":985,"end":999},"obj":"NN"},{"id":"T6863","span":{"begin":1000,"end":1002},"obj":"IN"},{"id":"T6864","span":{"begin":1003,"end":1006},"obj":"NN"},{"id":"T6865","span":{"begin":1007,"end":1014},"obj":"NN"},{"id":"T6866","span":{"begin":1015,"end":1023},"obj":"NN"},{"id":"T6867","span":{"begin":1023,"end":1024},"obj":"-COMMA-"},{"id":"T6868","span":{"begin":1025,"end":1032},"obj":"JJ"},{"id":"T6869","span":{"begin":1033,"end":1035},"obj":"TO"},{"id":"T6870","span":{"begin":1036,"end":1039},"obj":"DT"},{"id":"T6871","span":{"begin":1040,"end":1048},"obj":"VB"},{"id":"T6872","span":{"begin":1049,"end":1063},"obj":"NN"},{"id":"T6873","span":{"begin":1064,"end":1066},"obj":"IN"},{"id":"T6874","span":{"begin":1067,"end":1072},"obj":"NN"},{"id":"T6875","span":{"begin":1073,"end":1088},"obj":"NN"},{"id":"T6876","span":{"begin":1089,"end":1090},"obj":"-LRB-"},{"id":"T6877","span":{"begin":1090,"end":1096},"obj":"NN"},{"id":"T6878","span":{"begin":1097,"end":1099},"obj":"NN"},{"id":"T6879","span":{"begin":1099,"end":1100},"obj":"-RRB-"}],"relations":[{"id":"R5571","pred":"arg1Of","subj":"T6696","obj":"T6697"},{"id":"R5572","pred":"arg2Of","subj":"T6700","obj":"T6697"},{"id":"R5573","pred":"arg1Of","subj":"T6700","obj":"T6698"},{"id":"R5574","pred":"arg1Of","subj":"T6700","obj":"T6699"},{"id":"R5575","pred":"arg1Of","subj":"T6702","obj":"T6703"},{"id":"R5576","pred":"arg2Of","subj":"T6703","obj":"T6701"},{"id":"R5577","pred":"arg1Of","subj":"T6703","obj":"T6704"},{"id":"R5578","pred":"arg1Of","subj":"T6705","obj":"T6706"},{"id":"R5579","pred":"arg2Of","subj":"T6706","obj":"T6704"},{"id":"R5580","pred":"arg1Of","subj":"T6706","obj":"T6708"},{"id":"R5581","pred":"arg2Of","subj":"T6707","obj":"T6706"},{"id":"R5582","pred":"arg1Of","subj":"T6709","obj":"T6710"},{"id":"R5583","pred":"arg2Of","subj":"T6711","obj":"T6710"},{"id":"R5584","pred":"arg2Of","subj":"T6713","obj":"T6708"},{"id":"R5585","pred":"arg1Of","subj":"T6713","obj":"T6709"},{"id":"R5586","pred":"arg1Of","subj":"T6713","obj":"T6711"},{"id":"R5587","pred":"arg1Of","subj":"T6713","obj":"T6712"},{"id":"R5588","pred":"arg1Of","subj":"T6713","obj":"T6714"},{"id":"R5589","pred":"arg2Of","subj":"T6715","obj":"T6714"},{"id":"R5590","pred":"arg1Of","subj":"T6717","obj":"T6719"},{"id":"R5591","pred":"arg1Of","subj":"T6719","obj":"T6701"},{"id":"R5592","pred":"arg1Of","subj":"T6719","obj":"T6716"},{"id":"R5593","pred":"arg1Of","subj":"T6719","obj":"T6718"},{"id":"R5594","pred":"arg2Of","subj":"T6721","obj":"T6719"},{"id":"R5595","pred":"arg1Of","subj":"T6721","obj":"T6720"},{"id":"R5596","pred":"arg1Of","subj":"T6721","obj":"T6722"},{"id":"R5597","pred":"arg1Of","subj":"T6721","obj":"T6724"},{"id":"R5598","pred":"arg2Of","subj":"T6723","obj":"T6722"},{"id":"R5599","pred":"arg2Of","subj":"T6725","obj":"T6724"},{"id":"R5600","pred":"arg1Of","subj":"T6728","obj":"T6726"},{"id":"R5601","pred":"arg1Of","subj":"T6728","obj":"T6727"},{"id":"R5602","pred":"arg1Of","subj":"T6728","obj":"T6729"},{"id":"R5603","pred":"arg2Of","subj":"T6728","obj":"T6731"},{"id":"R5604","pred":"arg1Of","subj":"T6728","obj":"T6736"},{"id":"R5605","pred":"arg2Of","subj":"T6731","obj":"T6729"},{"id":"R5606","pred":"arg1Of","subj":"T6731","obj":"T6730"},{"id":"R5607","pred":"arg1Of","subj":"T6731","obj":"T6732"},{"id":"R5608","pred":"arg1Of","subj":"T6731","obj":"T6735"},{"id":"R5609","pred":"arg2Of","subj":"T6734","obj":"T6732"},{"id":"R5610","pred":"arg1Of","subj":"T6734","obj":"T6733"},{"id":"R5611","pred":"arg2Of","subj":"T6736","obj":"T6735"},{"id":"R5612","pred":"arg1Of","subj":"T6736","obj":"T6737"},{"id":"R5613","pred":"arg2Of","subj":"T6738","obj":"T6737"},{"id":"R5614","pred":"arg1Of","subj":"T6738","obj":"T6739"},{"id":"R5615","pred":"arg2Of","subj":"T6742","obj":"T6739"},{"id":"R5616","pred":"arg1Of","subj":"T6742","obj":"T6740"},{"id":"R5617","pred":"arg1Of","subj":"T6742","obj":"T6741"},{"id":"R5618","pred":"arg1Of","subj":"T6742","obj":"T6743"},{"id":"R5619","pred":"arg1Of","subj":"T6742","obj":"T6747"},{"id":"R5620","pred":"arg1Of","subj":"T6744","obj":"T6745"},{"id":"R5621","pred":"arg2Of","subj":"T6745","obj":"T6743"},{"id":"R5622","pred":"arg2Of","subj":"T6746","obj":"T6745"},{"id":"R5623","pred":"arg2Of","subj":"T6748","obj":"T6747"},{"id":"R5624","pred":"arg1Of","subj":"T6748","obj":"T6749"},{"id":"R5625","pred":"arg1Of","subj":"T6748","obj":"T6750"},{"id":"R5626","pred":"arg3Of","subj":"T6751","obj":"T6739"},{"id":"R5627","pred":"arg1Of","subj":"T6753","obj":"T6752"},{"id":"R5628","pred":"arg1Of","subj":"T6753","obj":"T6754"},{"id":"R5629","pred":"arg1Of","subj":"T6753","obj":"T6757"},{"id":"R5630","pred":"arg2Of","subj":"T6755","obj":"T6754"},{"id":"R5631","pred":"arg1Of","subj":"T6757","obj":"T6756"},{"id":"R5632","pred":"arg2Of","subj":"T6760","obj":"T6757"},{"id":"R5633","pred":"arg1Of","subj":"T6760","obj":"T6758"},{"id":"R5634","pred":"arg1Of","subj":"T6760","obj":"T6759"},{"id":"R5635","pred":"arg1Of","subj":"T6760","obj":"T6761"},{"id":"R5636","pred":"arg2Of","subj":"T6762","obj":"T6761"},{"id":"R5637","pred":"arg1Of","subj":"T6762","obj":"T6763"},{"id":"R5638","pred":"arg1Of","subj":"T6762","obj":"T6764"},{"id":"R5639","pred":"arg1Of","subj":"T6762","obj":"T6765"},{"id":"R5640","pred":"arg2Of","subj":"T6766","obj":"T6765"},{"id":"R5641","pred":"arg1Of","subj":"T6766","obj":"T6767"},{"id":"R5642","pred":"arg3Of","subj":"T6768","obj":"T6761"},{"id":"R5643","pred":"arg2Of","subj":"T6772","obj":"T6769"},{"id":"R5644","pred":"arg1Of","subj":"T6772","obj":"T6770"},{"id":"R5645","pred":"arg1Of","subj":"T6772","obj":"T6771"},{"id":"R5646","pred":"arg1Of","subj":"T6777","obj":"T6774"},{"id":"R5647","pred":"arg1Of","subj":"T6777","obj":"T6775"},{"id":"R5648","pred":"arg1Of","subj":"T6777","obj":"T6776"},{"id":"R5649","pred":"arg1Of","subj":"T6777","obj":"T6778"},{"id":"R5650","pred":"arg2Of","subj":"T6777","obj":"T6779"},{"id":"R5651","pred":"arg1Of","subj":"T6779","obj":"T6769"},{"id":"R5652","pred":"arg1Of","subj":"T6779","obj":"T6773"},{"id":"R5653","pred":"arg2Of","subj":"T6779","obj":"T6778"},{"id":"R5654","pred":"arg1Of","subj":"T6779","obj":"T6780"},{"id":"R5655","pred":"arg2Of","subj":"T6782","obj":"T6780"},{"id":"R5656","pred":"arg1Of","subj":"T6782","obj":"T6781"},{"id":"R5657","pred":"arg1Of","subj":"T6782","obj":"T6783"},{"id":"R5658","pred":"arg2Of","subj":"T6786","obj":"T6783"},{"id":"R5659","pred":"arg1Of","subj":"T6786","obj":"T6784"},{"id":"R5660","pred":"arg1Of","subj":"T6786","obj":"T6785"},{"id":"R5661","pred":"arg1Of","subj":"T6786","obj":"T6787"},{"id":"R5662","pred":"arg1Of","subj":"T6786","obj":"T6791"},{"id":"R5663","pred":"arg1Of","subj":"T6788","obj":"T6789"},{"id":"R5664","pred":"arg2Of","subj":"T6789","obj":"T6787"},{"id":"R5665","pred":"arg2Of","subj":"T6790","obj":"T6789"},{"id":"R5666","pred":"arg2Of","subj":"T6792","obj":"T6791"},{"id":"R5667","pred":"arg1Of","subj":"T6792","obj":"T6793"},{"id":"R5668","pred":"arg1Of","subj":"T6792","obj":"T6794"},{"id":"R5669","pred":"arg3Of","subj":"T6795","obj":"T6783"},{"id":"R5670","pred":"arg1Of","subj":"T6796","obj":"T6798"},{"id":"R5671","pred":"arg1Of","subj":"T6798","obj":"T6797"},{"id":"R5672","pred":"arg2Of","subj":"T6803","obj":"T6798"},{"id":"R5673","pred":"arg1Of","subj":"T6803","obj":"T6799"},{"id":"R5674","pred":"arg1Of","subj":"T6803","obj":"T6800"},{"id":"R5675","pred":"arg1Of","subj":"T6803","obj":"T6801"},{"id":"R5676","pred":"arg1Of","subj":"T6803","obj":"T6802"},{"id":"R5677","pred":"arg1Of","subj":"T6803","obj":"T6804"},{"id":"R5678","pred":"arg2Of","subj":"T6806","obj":"T6804"},{"id":"R5679","pred":"arg1Of","subj":"T6806","obj":"T6805"},{"id":"R5680","pred":"arg1Of","subj":"T6806","obj":"T6807"},{"id":"R5681","pred":"arg1Of","subj":"T6809","obj":"T6808"},{"id":"R5682","pred":"arg1Of","subj":"T6809","obj":"T6810"},{"id":"R5683","pred":"arg2Of","subj":"T6810","obj":"T6807"},{"id":"R5684","pred":"arg2Of","subj":"T6812","obj":"T6810"},{"id":"R5685","pred":"arg1Of","subj":"T6812","obj":"T6811"},{"id":"R5686","pred":"arg1Of","subj":"T6812","obj":"T6813"},{"id":"R5687","pred":"arg2Of","subj":"T6816","obj":"T6813"},{"id":"R5688","pred":"arg1Of","subj":"T6816","obj":"T6814"},{"id":"R5689","pred":"arg1Of","subj":"T6816","obj":"T6815"},{"id":"R5690","pred":"arg1Of","subj":"T6816","obj":"T6817"},{"id":"R5691","pred":"arg1Of","subj":"T6816","obj":"T6821"},{"id":"R5692","pred":"arg1Of","subj":"T6816","obj":"T6824"},{"id":"R5693","pred":"arg1Of","subj":"T6818","obj":"T6819"},{"id":"R5694","pred":"arg2Of","subj":"T6819","obj":"T6817"},{"id":"R5695","pred":"arg2Of","subj":"T6820","obj":"T6819"},{"id":"R5696","pred":"arg2Of","subj":"T6822","obj":"T6821"},{"id":"R5697","pred":"arg1Of","subj":"T6822","obj":"T6823"},{"id":"R5698","pred":"arg2Of","subj":"T6826","obj":"T6824"},{"id":"R5699","pred":"arg1Of","subj":"T6826","obj":"T6825"},{"id":"R5700","pred":"arg3Of","subj":"T6827","obj":"T6813"},{"id":"R5701","pred":"arg1Of","subj":"T6828","obj":"T6829"},{"id":"R5702","pred":"arg2Of","subj":"T6828","obj":"T6831"},{"id":"R5703","pred":"arg2Of","subj":"T6831","obj":"T6829"},{"id":"R5704","pred":"arg1Of","subj":"T6831","obj":"T6830"},{"id":"R5705","pred":"arg3Of","subj":"T6833","obj":"T6831"},{"id":"R5706","pred":"arg1Of","subj":"T6833","obj":"T6832"},{"id":"R5707","pred":"arg1Of","subj":"T6836","obj":"T6834"},{"id":"R5708","pred":"arg1Of","subj":"T6836","obj":"T6835"},{"id":"R5709","pred":"arg1Of","subj":"T6836","obj":"T6837"},{"id":"R5710","pred":"arg1Of","subj":"T6836","obj":"T6840"},{"id":"R5711","pred":"arg2Of","subj":"T6839","obj":"T6837"},{"id":"R5712","pred":"arg1Of","subj":"T6839","obj":"T6838"},{"id":"R5713","pred":"arg2Of","subj":"T6840","obj":"T6833"},{"id":"R5714","pred":"arg1Of","subj":"T6840","obj":"T6841"},{"id":"R5715","pred":"arg1Of","subj":"T6840","obj":"T6844"},{"id":"R5716","pred":"arg2Of","subj":"T6843","obj":"T6841"},{"id":"R5717","pred":"arg1Of","subj":"T6843","obj":"T6842"},{"id":"R5718","pred":"arg2Of","subj":"T6848","obj":"T6844"},{"id":"R5719","pred":"arg1Of","subj":"T6848","obj":"T6845"},{"id":"R5720","pred":"arg1Of","subj":"T6848","obj":"T6846"},{"id":"R5721","pred":"arg1Of","subj":"T6848","obj":"T6847"},{"id":"R5722","pred":"arg1Of","subj":"T6848","obj":"T6849"},{"id":"R5723","pred":"arg2Of","subj":"T6850","obj":"T6849"},{"id":"R5724","pred":"arg1Of","subj":"T6852","obj":"T6851"},{"id":"R5725","pred":"arg1Of","subj":"T6852","obj":"T6853"},{"id":"R5726","pred":"arg1Of","subj":"T6852","obj":"T6855"},{"id":"R5727","pred":"arg1Of","subj":"T6852","obj":"T6859"},{"id":"R5728","pred":"arg2Of","subj":"T6854","obj":"T6853"},{"id":"R5729","pred":"arg2Of","subj":"T6858","obj":"T6855"},{"id":"R5730","pred":"arg1Of","subj":"T6858","obj":"T6856"},{"id":"R5731","pred":"arg1Of","subj":"T6858","obj":"T6857"},{"id":"R5732","pred":"arg1Of","subj":"T6859","obj":"T6860"},{"id":"R5733","pred":"arg1Of","subj":"T6859","obj":"T6867"},{"id":"R5734","pred":"arg2Of","subj":"T6862","obj":"T6860"},{"id":"R5735","pred":"arg1Of","subj":"T6862","obj":"T6861"},{"id":"R5736","pred":"arg1Of","subj":"T6862","obj":"T6863"},{"id":"R5737","pred":"arg2Of","subj":"T6866","obj":"T6863"},{"id":"R5738","pred":"arg1Of","subj":"T6866","obj":"T6864"},{"id":"R5739","pred":"arg1Of","subj":"T6866","obj":"T6865"},{"id":"R5740","pred":"arg2Of","subj":"T6868","obj":"T6859"},{"id":"R5741","pred":"arg1Of","subj":"T6868","obj":"T6869"},{"id":"R5742","pred":"arg2Of","subj":"T6872","obj":"T6869"},{"id":"R5743","pred":"arg1Of","subj":"T6872","obj":"T6870"},{"id":"R5744","pred":"arg2Of","subj":"T6872","obj":"T6871"},{"id":"R5745","pred":"arg1Of","subj":"T6872","obj":"T6873"},{"id":"R5746","pred":"arg2Of","subj":"T6875","obj":"T6873"},{"id":"R5747","pred":"arg1Of","subj":"T6875","obj":"T6874"},{"id":"R5748","pred":"arg1Of","subj":"T6875","obj":"T6876"},{"id":"R5749","pred":"arg2Of","subj":"T6878","obj":"T6876"},{"id":"R5750","pred":"arg1Of","subj":"T6878","obj":"T6877"},{"id":"R5751","pred":"arg3Of","subj":"T6879","obj":"T6876"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T6505","span":{"begin":0,"end":3},"obj":"NNP"},{"id":"T6506","span":{"begin":4,"end":13},"obj":"VBZ"},{"id":"T6507","span":{"begin":14,"end":25},"obj":"JJ"},{"id":"T6508","span":{"begin":26,"end":31},"obj":"NN"},{"id":"T6509","span":{"begin":32,"end":42},"obj":"NN"},{"id":"T6510","span":{"begin":43,"end":48},"obj":"IN"},{"id":"T6511","span":{"begin":49,"end":64},"obj":"NN"},{"id":"T6512","span":{"begin":65,"end":70},"obj":"VBZ"},{"id":"T6513","span":{"begin":71,"end":73},"obj":"TO"},{"id":"T6514","span":{"begin":74,"end":88},"obj":"NN"},{"id":"T6515","span":{"begin":89,"end":92},"obj":"CC"},{"id":"T6516","span":{"begin":93,"end":104},"obj":"NN"},{"id":"T6517","span":{"begin":105,"end":107},"obj":"IN"},{"id":"T6518","span":{"begin":108,"end":113},"obj":"NNP"},{"id":"T6519","span":{"begin":114,"end":117},"obj":"CC"},{"id":"T6520","span":{"begin":118,"end":128},"obj":"JJ"},{"id":"T6521","span":{"begin":129,"end":136},"obj":"JJ"},{"id":"T6522","span":{"begin":137,"end":150},"obj":"NN"},{"id":"T6523","span":{"begin":151,"end":153},"obj":"IN"},{"id":"T6524","span":{"begin":154,"end":159},"obj":"NNP"},{"id":"T6525","span":{"begin":159,"end":160},"obj":","},{"id":"T6526","span":{"begin":161,"end":163},"obj":"PRP"},{"id":"T6527","span":{"begin":164,"end":169},"obj":"RB"},{"id":"T6528","span":{"begin":170,"end":178},"obj":"VBD"},{"id":"T6529","span":{"begin":179,"end":182},"obj":"DT"},{"id":"T6530","span":{"begin":183,"end":190},"obj":"NNS"},{"id":"T6531","span":{"begin":191,"end":193},"obj":"IN"},{"id":"T6532","span":{"begin":194,"end":197},"obj":"NNP"},{"id":"T6533","span":{"begin":198,"end":200},"obj":"IN"},{"id":"T6534","span":{"begin":201,"end":206},"obj":"NNP"},{"id":"T6535","span":{"begin":206,"end":207},"obj":"."},{"id":"T6536","span":{"begin":208,"end":219},"obj":"NNP"},{"id":"T6537","span":{"begin":220,"end":225},"obj":"NNP"},{"id":"T6538","span":{"begin":226,"end":241},"obj":"NN"},{"id":"T6539","span":{"begin":242,"end":245},"obj":"VBD"},{"id":"T6540","span":{"begin":246,"end":254},"obj":"RB"},{"id":"T6541","span":{"begin":255,"end":264},"obj":"VBN"},{"id":"T6542","span":{"begin":265,"end":274},"obj":"VBG"},{"id":"T6543","span":{"begin":275,"end":278},"obj":"NNP"},{"id":"T6544","span":{"begin":279,"end":290},"obj":"NN"},{"id":"T6545","span":{"begin":291,"end":295},"obj":"WRB"},{"id":"T6546","span":{"begin":296,"end":304},"obj":"VBN"},{"id":"T6547","span":{"begin":305,"end":307},"obj":"TO"},{"id":"T6548","span":{"begin":308,"end":315},"obj":"VB"},{"id":"T6549","span":{"begin":316,"end":317},"obj":"-LRB-"},{"id":"T6550","span":{"begin":317,"end":320},"obj":"CD"},{"id":"T6551","span":{"begin":321,"end":322},"obj":"CD"},{"id":"T6552","span":{"begin":323,"end":324},"obj":"CD"},{"id":"T6553","span":{"begin":325,"end":328},"obj":"IN"},{"id":"T6554","span":{"begin":329,"end":331},"obj":"CD"},{"id":"T6555","span":{"begin":332,"end":333},"obj":"CD"},{"id":"T6556","span":{"begin":334,"end":336},"obj":"CD"},{"id":"T6557","span":{"begin":336,"end":337},"obj":":"},{"id":"T6558","span":{"begin":338,"end":339},"obj":"NNP"},{"id":"T6559","span":{"begin":340,"end":341},"obj":"NNP"},{"id":"T6560","span":{"begin":342,"end":346},"obj":"CD"},{"id":"T6561","span":{"begin":346,"end":347},"obj":"-RRB-"},{"id":"T6562","span":{"begin":347,"end":348},"obj":"."},{"id":"T6563","span":{"begin":349,"end":352},"obj":"DT"},{"id":"T6564","span":{"begin":353,"end":361},"obj":"NN"},{"id":"T6565","span":{"begin":362,"end":364},"obj":"IN"},{"id":"T6566","span":{"begin":365,"end":368},"obj":"NNP"},{"id":"T6567","span":{"begin":369,"end":382},"obj":"RB"},{"id":"T6568","span":{"begin":383,"end":396},"obj":"VBD"},{"id":"T6569","span":{"begin":397,"end":408},"obj":"JJ"},{"id":"T6570","span":{"begin":409,"end":414},"obj":"JJ"},{"id":"T6571","span":{"begin":415,"end":430},"obj":"NN"},{"id":"T6572","span":{"begin":431,"end":432},"obj":"-LRB-"},{"id":"T6573","span":{"begin":432,"end":433},"obj":"NNP"},{"id":"T6574","span":{"begin":434,"end":435},"obj":"SYM"},{"id":"T6575","span":{"begin":436,"end":440},"obj":"CD"},{"id":"T6576","span":{"begin":440,"end":441},"obj":":"},{"id":"T6577","span":{"begin":442,"end":448},"obj":"NN"},{"id":"T6578","span":{"begin":449,"end":450},"obj":"CD"},{"id":"T6579","span":{"begin":450,"end":451},"obj":"-RRB-"},{"id":"T6580","span":{"begin":452,"end":454},"obj":"IN"},{"id":"T6581","span":{"begin":455,"end":456},"obj":"DT"},{"id":"T6582","span":{"begin":457,"end":464},"obj":"JJ"},{"id":"T6583","span":{"begin":465,"end":472},"obj":"NN"},{"id":"T6584","span":{"begin":472,"end":473},"obj":","},{"id":"T6585","span":{"begin":474,"end":481},"obj":"JJ"},{"id":"T6586","span":{"begin":482,"end":487},"obj":"NNP"},{"id":"T6587","span":{"begin":488,"end":491},"obj":"CD"},{"id":"T6588","span":{"begin":492,"end":507},"obj":"NN"},{"id":"T6589","span":{"begin":508,"end":511},"obj":"VBD"},{"id":"T6590","span":{"begin":512,"end":521},"obj":"VBN"},{"id":"T6591","span":{"begin":522,"end":531},"obj":"VBG"},{"id":"T6592","span":{"begin":532,"end":535},"obj":"NNP"},{"id":"T6593","span":{"begin":536,"end":547},"obj":"NN"},{"id":"T6594","span":{"begin":548,"end":549},"obj":"-LRB-"},{"id":"T6595","span":{"begin":549,"end":552},"obj":"CD"},{"id":"T6596","span":{"begin":553,"end":554},"obj":"CD"},{"id":"T6597","span":{"begin":555,"end":556},"obj":"CD"},{"id":"T6598","span":{"begin":557,"end":560},"obj":"IN"},{"id":"T6599","span":{"begin":561,"end":563},"obj":"CD"},{"id":"T6600","span":{"begin":564,"end":565},"obj":"CD"},{"id":"T6601","span":{"begin":566,"end":568},"obj":"CD"},{"id":"T6602","span":{"begin":568,"end":569},"obj":":"},{"id":"T6603","span":{"begin":570,"end":571},"obj":"NNP"},{"id":"T6604","span":{"begin":572,"end":573},"obj":"NNP"},{"id":"T6605","span":{"begin":574,"end":578},"obj":"CD"},{"id":"T6606","span":{"begin":578,"end":579},"obj":"-RRB-"},{"id":"T6607","span":{"begin":579,"end":580},"obj":"."},{"id":"T6608","span":{"begin":581,"end":584},"obj":"NNP"},{"id":"T6609","span":{"begin":585,"end":594},"obj":"RB"},{"id":"T6610","span":{"begin":595,"end":604},"obj":"VBD"},{"id":"T6611","span":{"begin":605,"end":616},"obj":"JJ"},{"id":"T6612","span":{"begin":617,"end":622},"obj":"NNP"},{"id":"T6613","span":{"begin":623,"end":626},"obj":"CD"},{"id":"T6614","span":{"begin":627,"end":634},"obj":"JJ"},{"id":"T6615","span":{"begin":635,"end":650},"obj":"NN"},{"id":"T6616","span":{"begin":650,"end":651},"obj":","},{"id":"T6617","span":{"begin":652,"end":653},"obj":"DT"},{"id":"T6618","span":{"begin":654,"end":660},"obj":"NN"},{"id":"T6619","span":{"begin":661,"end":663},"obj":"IN"},{"id":"T6620","span":{"begin":664,"end":669},"obj":"JJ"},{"id":"T6621","span":{"begin":670,"end":680},"obj":"NN"},{"id":"T6622","span":{"begin":681,"end":684},"obj":"CC"},{"id":"T6623","span":{"begin":685,"end":692},"obj":"JJ"},{"id":"T6624","span":{"begin":693,"end":706},"obj":"NN"},{"id":"T6625","span":{"begin":707,"end":708},"obj":"-LRB-"},{"id":"T6626","span":{"begin":708,"end":711},"obj":"CD"},{"id":"T6627","span":{"begin":712,"end":713},"obj":"CD"},{"id":"T6628","span":{"begin":714,"end":715},"obj":"CD"},{"id":"T6629","span":{"begin":716,"end":719},"obj":"IN"},{"id":"T6630","span":{"begin":720,"end":722},"obj":"CD"},{"id":"T6631","span":{"begin":723,"end":724},"obj":"NN"},{"id":"T6632","span":{"begin":725,"end":726},"obj":"CD"},{"id":"T6633","span":{"begin":726,"end":727},"obj":":"},{"id":"T6634","span":{"begin":728,"end":729},"obj":"NNP"},{"id":"T6635","span":{"begin":730,"end":731},"obj":"SYM"},{"id":"T6636","span":{"begin":731,"end":735},"obj":"CD"},{"id":"T6637","span":{"begin":735,"end":736},"obj":":"},{"id":"T6638","span":{"begin":737,"end":743},"obj":"NNP"},{"id":"T6639","span":{"begin":744,"end":746},"obj":"NNP"},{"id":"T6640","span":{"begin":746,"end":747},"obj":"-RRB-"},{"id":"T6641","span":{"begin":747,"end":748},"obj":"."},{"id":"T6642","span":{"begin":749,"end":754},"obj":"NNS"},{"id":"T6643","span":{"begin":755,"end":759},"obj":"VBD"},{"id":"T6644","span":{"begin":760,"end":764},"obj":"RB"},{"id":"T6645","span":{"begin":765,"end":774},"obj":"VBN"},{"id":"T6646","span":{"begin":775,"end":777},"obj":"TO"},{"id":"T6647","span":{"begin":778,"end":784},"obj":"VB"},{"id":"T6648","span":{"begin":785,"end":788},"obj":"DT"},{"id":"T6649","span":{"begin":789,"end":800},"obj":"JJ"},{"id":"T6650","span":{"begin":801,"end":812},"obj":"NNS"},{"id":"T6651","span":{"begin":813,"end":815},"obj":"IN"},{"id":"T6652","span":{"begin":816,"end":823},"obj":"JJ"},{"id":"T6653","span":{"begin":824,"end":839},"obj":"NN"},{"id":"T6654","span":{"begin":840,"end":848},"obj":"VBD"},{"id":"T6655","span":{"begin":849,"end":851},"obj":"IN"},{"id":"T6656","span":{"begin":852,"end":859},"obj":"JJ"},{"id":"T6657","span":{"begin":860,"end":867},"obj":"NNS"},{"id":"T6658","span":{"begin":868,"end":870},"obj":"IN"},{"id":"T6659","span":{"begin":871,"end":874},"obj":"DT"},{"id":"T6660","span":{"begin":875,"end":878},"obj":"NNP"},{"id":"T6661","span":{"begin":879,"end":886},"obj":"JJ"},{"id":"T6662","span":{"begin":887,"end":895},"obj":"NN"},{"id":"T6663","span":{"begin":896,"end":898},"obj":"IN"},{"id":"T6664","span":{"begin":899,"end":904},"obj":"NN"},{"id":"T6665","span":{"begin":904,"end":905},"obj":"."},{"id":"T6666","span":{"begin":906,"end":909},"obj":"DT"},{"id":"T6667","span":{"begin":910,"end":918},"obj":"NN"},{"id":"T6668","span":{"begin":919,"end":921},"obj":"IN"},{"id":"T6669","span":{"begin":922,"end":925},"obj":"NNP"},{"id":"T6670","span":{"begin":926,"end":928},"obj":"TO"},{"id":"T6671","span":{"begin":929,"end":943},"obj":"JJ"},{"id":"T6672","span":{"begin":944,"end":955},"obj":"NN"},{"id":"T6673","span":{"begin":956,"end":961},"obj":"NNS"},{"id":"T6674","span":{"begin":962,"end":970},"obj":"VBD"},{"id":"T6675","span":{"begin":971,"end":973},"obj":"IN"},{"id":"T6676","span":{"begin":974,"end":984},"obj":"JJ"},{"id":"T6677","span":{"begin":985,"end":999},"obj":"NN"},{"id":"T6678","span":{"begin":1000,"end":1002},"obj":"IN"},{"id":"T6679","span":{"begin":1003,"end":1006},"obj":"NNP"},{"id":"T6680","span":{"begin":1007,"end":1014},"obj":"JJ"},{"id":"T6681","span":{"begin":1015,"end":1023},"obj":"NN"},{"id":"T6682","span":{"begin":1023,"end":1024},"obj":","},{"id":"T6683","span":{"begin":1025,"end":1032},"obj":"JJ"},{"id":"T6684","span":{"begin":1033,"end":1035},"obj":"TO"},{"id":"T6685","span":{"begin":1036,"end":1039},"obj":"DT"},{"id":"T6686","span":{"begin":1040,"end":1048},"obj":"JJ"},{"id":"T6687","span":{"begin":1049,"end":1063},"obj":"NN"},{"id":"T6688","span":{"begin":1064,"end":1066},"obj":"IN"},{"id":"T6689","span":{"begin":1067,"end":1072},"obj":"JJ"},{"id":"T6690","span":{"begin":1073,"end":1088},"obj":"NN"},{"id":"T6691","span":{"begin":1089,"end":1090},"obj":"-LRB-"},{"id":"T6692","span":{"begin":1090,"end":1096},"obj":"NN"},{"id":"T6693","span":{"begin":1097,"end":1099},"obj":"NN"},{"id":"T6694","span":{"begin":1099,"end":1100},"obj":"-RRB-"},{"id":"T6695","span":{"begin":1100,"end":1101},"obj":"."}],"relations":[{"id":"R5380","pred":"nsubj","subj":"T6505","obj":"T6506"},{"id":"R5381","pred":"ROOT","subj":"T6506","obj":"T6506"},{"id":"R5382","pred":"amod","subj":"T6507","obj":"T6509"},{"id":"R5383","pred":"compound","subj":"T6508","obj":"T6509"},{"id":"R5384","pred":"dobj","subj":"T6509","obj":"T6506"},{"id":"R5385","pred":"mark","subj":"T6510","obj":"T6512"},{"id":"R5386","pred":"nsubj","subj":"T6511","obj":"T6512"},{"id":"R5387","pred":"advcl","subj":"T6512","obj":"T6528"},{"id":"R5388","pred":"prep","subj":"T6513","obj":"T6512"},{"id":"R5389","pred":"pobj","subj":"T6514","obj":"T6513"},{"id":"R5390","pred":"cc","subj":"T6515","obj":"T6514"},{"id":"R5391","pred":"conj","subj":"T6516","obj":"T6514"},{"id":"R5392","pred":"prep","subj":"T6517","obj":"T6516"},{"id":"R5393","pred":"pobj","subj":"T6518","obj":"T6517"},{"id":"R5394","pred":"cc","subj":"T6519","obj":"T6518"},{"id":"R5395","pred":"amod","subj":"T6520","obj":"T6522"},{"id":"R5396","pred":"amod","subj":"T6521","obj":"T6522"},{"id":"R5397","pred":"conj","subj":"T6522","obj":"T6518"},{"id":"R5398","pred":"prep","subj":"T6523","obj":"T6522"},{"id":"R5399","pred":"pobj","subj":"T6524","obj":"T6523"},{"id":"R5400","pred":"punct","subj":"T6525","obj":"T6528"},{"id":"R5401","pred":"nsubj","subj":"T6526","obj":"T6528"},{"id":"R5402","pred":"advmod","subj":"T6527","obj":"T6528"},{"id":"R5403","pred":"advcl","subj":"T6528","obj":"T6506"},{"id":"R5404","pred":"det","subj":"T6529","obj":"T6530"},{"id":"R5405","pred":"dobj","subj":"T6530","obj":"T6528"},{"id":"R5406","pred":"prep","subj":"T6531","obj":"T6530"},{"id":"R5407","pred":"pobj","subj":"T6532","obj":"T6531"},{"id":"R5408","pred":"prep","subj":"T6533","obj":"T6530"},{"id":"R5409","pred":"pobj","subj":"T6534","obj":"T6533"},{"id":"R5410","pred":"punct","subj":"T6535","obj":"T6506"},{"id":"R5411","pred":"compound","subj":"T6536","obj":"T6538"},{"id":"R5412","pred":"compound","subj":"T6537","obj":"T6538"},{"id":"R5413","pred":"nsubjpass","subj":"T6538","obj":"T6541"},{"id":"R5414","pred":"auxpass","subj":"T6539","obj":"T6541"},{"id":"R5415","pred":"advmod","subj":"T6540","obj":"T6541"},{"id":"R5416","pred":"ROOT","subj":"T6541","obj":"T6541"},{"id":"R5417","pred":"prep","subj":"T6542","obj":"T6541"},{"id":"R5418","pred":"compound","subj":"T6543","obj":"T6544"},{"id":"R5419","pred":"pobj","subj":"T6544","obj":"T6542"},{"id":"R5420","pred":"advmod","subj":"T6545","obj":"T6546"},{"id":"R5421","pred":"advcl","subj":"T6546","obj":"T6541"},{"id":"R5422","pred":"aux","subj":"T6547","obj":"T6548"},{"id":"R5423","pred":"xcomp","subj":"T6548","obj":"T6546"},{"id":"R5424","pred":"punct","subj":"T6549","obj":"T6552"},{"id":"R5425","pred":"nummod","subj":"T6550","obj":"T6552"},{"id":"R5426","pred":"nummod","subj":"T6551","obj":"T6552"},{"id":"R5427","pred":"dobj","subj":"T6552","obj":"T6548"},{"id":"R5428","pred":"prep","subj":"T6553","obj":"T6552"},{"id":"R5429","pred":"nummod","subj":"T6554","obj":"T6556"},{"id":"R5430","pred":"nummod","subj":"T6555","obj":"T6556"},{"id":"R5431","pred":"pobj","subj":"T6556","obj":"T6553"},{"id":"R5432","pred":"punct","subj":"T6557","obj":"T6560"},{"id":"R5433","pred":"compound","subj":"T6558","obj":"T6559"},{"id":"R5434","pred":"compound","subj":"T6559","obj":"T6560"},{"id":"R5435","pred":"npadvmod","subj":"T6560","obj":"T6541"},{"id":"R5436","pred":"punct","subj":"T6561","obj":"T6560"},{"id":"R5437","pred":"punct","subj":"T6562","obj":"T6541"},{"id":"R5438","pred":"det","subj":"T6563","obj":"T6564"},{"id":"R5439","pred":"nsubj","subj":"T6564","obj":"T6568"},{"id":"R5440","pred":"prep","subj":"T6565","obj":"T6564"},{"id":"R5441","pred":"pobj","subj":"T6566","obj":"T6565"},{"id":"R5442","pred":"advmod","subj":"T6567","obj":"T6568"},{"id":"R5443","pred":"ROOT","subj":"T6568","obj":"T6568"},{"id":"R5444","pred":"amod","subj":"T6569","obj":"T6571"},{"id":"R5445","pred":"amod","subj":"T6570","obj":"T6571"},{"id":"R5446","pred":"dobj","subj":"T6571","obj":"T6568"},{"id":"R5447","pred":"punct","subj":"T6572","obj":"T6573"},{"id":"R5448","pred":"appos","subj":"T6573","obj":"T6571"},{"id":"R5449","pred":"punct","subj":"T6574","obj":"T6573"},{"id":"R5450","pred":"nummod","subj":"T6575","obj":"T6573"},{"id":"R5451","pred":"punct","subj":"T6576","obj":"T6573"},{"id":"R5452","pred":"ROOT","subj":"T6577","obj":"T6577"},{"id":"R5453","pred":"nummod","subj":"T6578","obj":"T6577"},{"id":"R5454","pred":"punct","subj":"T6579","obj":"T6577"},{"id":"R5455","pred":"prep","subj":"T6580","obj":"T6590"},{"id":"R5456","pred":"det","subj":"T6581","obj":"T6583"},{"id":"R5457","pred":"amod","subj":"T6582","obj":"T6583"},{"id":"R5458","pred":"pobj","subj":"T6583","obj":"T6580"},{"id":"R5459","pred":"punct","subj":"T6584","obj":"T6590"},{"id":"R5460","pred":"amod","subj":"T6585","obj":"T6588"},{"id":"R5461","pred":"nmod","subj":"T6586","obj":"T6588"},{"id":"R5462","pred":"nummod","subj":"T6587","obj":"T6588"},{"id":"R5463","pred":"nsubjpass","subj":"T6588","obj":"T6590"},{"id":"R5464","pred":"auxpass","subj":"T6589","obj":"T6590"},{"id":"R5465","pred":"ROOT","subj":"T6590","obj":"T6590"},{"id":"R5466","pred":"prep","subj":"T6591","obj":"T6590"},{"id":"R5467","pred":"compound","subj":"T6592","obj":"T6593"},{"id":"R5468","pred":"pobj","subj":"T6593","obj":"T6591"},{"id":"R5469","pred":"punct","subj":"T6594","obj":"T6597"},{"id":"R5470","pred":"nummod","subj":"T6595","obj":"T6597"},{"id":"R5471","pred":"nummod","subj":"T6596","obj":"T6597"},{"id":"R5472","pred":"npadvmod","subj":"T6597","obj":"T6590"},{"id":"R5473","pred":"prep","subj":"T6598","obj":"T6597"},{"id":"R5474","pred":"nummod","subj":"T6599","obj":"T6601"},{"id":"R5475","pred":"nummod","subj":"T6600","obj":"T6601"},{"id":"R5476","pred":"pobj","subj":"T6601","obj":"T6598"},{"id":"R5477","pred":"punct","subj":"T6602","obj":"T6605"},{"id":"R5478","pred":"compound","subj":"T6603","obj":"T6604"},{"id":"R5479","pred":"compound","subj":"T6604","obj":"T6605"},{"id":"R5480","pred":"npadvmod","subj":"T6605","obj":"T6590"},{"id":"R5481","pred":"punct","subj":"T6606","obj":"T6605"},{"id":"R5482","pred":"punct","subj":"T6607","obj":"T6590"},{"id":"R5483","pred":"nsubj","subj":"T6608","obj":"T6610"},{"id":"R5484","pred":"advmod","subj":"T6609","obj":"T6610"},{"id":"R5485","pred":"ROOT","subj":"T6610","obj":"T6610"},{"id":"R5486","pred":"amod","subj":"T6611","obj":"T6615"},{"id":"R5487","pred":"nmod","subj":"T6612","obj":"T6615"},{"id":"R5488","pred":"nummod","subj":"T6613","obj":"T6612"},{"id":"R5489","pred":"amod","subj":"T6614","obj":"T6615"},{"id":"R5490","pred":"dobj","subj":"T6615","obj":"T6610"},{"id":"R5491","pred":"punct","subj":"T6616","obj":"T6615"},{"id":"R5492","pred":"det","subj":"T6617","obj":"T6618"},{"id":"R5493","pred":"appos","subj":"T6618","obj":"T6615"},{"id":"R5494","pred":"prep","subj":"T6619","obj":"T6618"},{"id":"R5495","pred":"amod","subj":"T6620","obj":"T6621"},{"id":"R5496","pred":"pobj","subj":"T6621","obj":"T6619"},{"id":"R5497","pred":"cc","subj":"T6622","obj":"T6621"},{"id":"R5498","pred":"amod","subj":"T6623","obj":"T6624"},{"id":"R5499","pred":"conj","subj":"T6624","obj":"T6621"},{"id":"R5500","pred":"punct","subj":"T6625","obj":"T6628"},{"id":"R5501","pred":"nummod","subj":"T6626","obj":"T6628"},{"id":"R5502","pred":"nummod","subj":"T6627","obj":"T6628"},{"id":"R5503","pred":"appos","subj":"T6628","obj":"T6615"},{"id":"R5504","pred":"quantmod","subj":"T6629","obj":"T6630"},{"id":"R5505","pred":"nummod","subj":"T6630","obj":"T6631"},{"id":"R5506","pred":"pobj","subj":"T6631","obj":"T6628"},{"id":"R5507","pred":"nummod","subj":"T6632","obj":"T6631"},{"id":"R5508","pred":"punct","subj":"T6633","obj":"T6631"},{"id":"R5509","pred":"compound","subj":"T6634","obj":"T6636"},{"id":"R5510","pred":"punct","subj":"T6635","obj":"T6636"},{"id":"R5511","pred":"conj","subj":"T6636","obj":"T6631"},{"id":"R5512","pred":"punct","subj":"T6637","obj":"T6636"},{"id":"R5513","pred":"compound","subj":"T6638","obj":"T6639"},{"id":"R5514","pred":"appos","subj":"T6639","obj":"T6636"},{"id":"R5515","pred":"punct","subj":"T6640","obj":"T6636"},{"id":"R5516","pred":"punct","subj":"T6641","obj":"T6631"},{"id":"R5517","pred":"nsubjpass","subj":"T6642","obj":"T6645"},{"id":"R5518","pred":"auxpass","subj":"T6643","obj":"T6645"},{"id":"R5519","pred":"advmod","subj":"T6644","obj":"T6645"},{"id":"R5520","pred":"ROOT","subj":"T6645","obj":"T6645"},{"id":"R5521","pred":"aux","subj":"T6646","obj":"T6647"},{"id":"R5522","pred":"advcl","subj":"T6647","obj":"T6645"},{"id":"R5523","pred":"det","subj":"T6648","obj":"T6650"},{"id":"R5524","pred":"amod","subj":"T6649","obj":"T6650"},{"id":"R5525","pred":"dobj","subj":"T6650","obj":"T6647"},{"id":"R5526","pred":"prep","subj":"T6651","obj":"T6650"},{"id":"R5527","pred":"amod","subj":"T6652","obj":"T6653"},{"id":"R5528","pred":"pobj","subj":"T6653","obj":"T6651"},{"id":"R5529","pred":"conj","subj":"T6654","obj":"T6645"},{"id":"R5530","pred":"prep","subj":"T6655","obj":"T6654"},{"id":"R5531","pred":"amod","subj":"T6656","obj":"T6657"},{"id":"R5532","pred":"pobj","subj":"T6657","obj":"T6655"},{"id":"R5533","pred":"prep","subj":"T6658","obj":"T6657"},{"id":"R5534","pred":"det","subj":"T6659","obj":"T6662"},{"id":"R5535","pred":"compound","subj":"T6660","obj":"T6662"},{"id":"R5536","pred":"amod","subj":"T6661","obj":"T6662"},{"id":"R5537","pred":"pobj","subj":"T6662","obj":"T6658"},{"id":"R5538","pred":"prep","subj":"T6663","obj":"T6662"},{"id":"R5539","pred":"pobj","subj":"T6664","obj":"T6663"},{"id":"R5540","pred":"punct","subj":"T6665","obj":"T6645"},{"id":"R5541","pred":"det","subj":"T6666","obj":"T6667"},{"id":"R5542","pred":"nsubj","subj":"T6667","obj":"T6674"},{"id":"R5543","pred":"prep","subj":"T6668","obj":"T6667"},{"id":"R5544","pred":"pobj","subj":"T6669","obj":"T6668"},{"id":"R5545","pred":"prep","subj":"T6670","obj":"T6667"},{"id":"R5546","pred":"amod","subj":"T6671","obj":"T6673"},{"id":"R5547","pred":"compound","subj":"T6672","obj":"T6673"},{"id":"R5548","pred":"pobj","subj":"T6673","obj":"T6670"},{"id":"R5549","pred":"ROOT","subj":"T6674","obj":"T6674"},{"id":"R5550","pred":"prep","subj":"T6675","obj":"T6674"},{"id":"R5551","pred":"amod","subj":"T6676","obj":"T6677"},{"id":"R5552","pred":"pobj","subj":"T6677","obj":"T6675"},{"id":"R5553","pred":"prep","subj":"T6678","obj":"T6677"},{"id":"R5554","pred":"pobj","subj":"T6679","obj":"T6678"},{"id":"R5555","pred":"amod","subj":"T6680","obj":"T6681"},{"id":"R5556","pred":"conj","subj":"T6681","obj":"T6677"},{"id":"R5557","pred":"punct","subj":"T6682","obj":"T6681"},{"id":"R5558","pred":"amod","subj":"T6683","obj":"T6681"},{"id":"R5559","pred":"prep","subj":"T6684","obj":"T6683"},{"id":"R5560","pred":"det","subj":"T6685","obj":"T6687"},{"id":"R5561","pred":"amod","subj":"T6686","obj":"T6687"},{"id":"R5562","pred":"pobj","subj":"T6687","obj":"T6684"},{"id":"R5563","pred":"prep","subj":"T6688","obj":"T6687"},{"id":"R5564","pred":"amod","subj":"T6689","obj":"T6690"},{"id":"R5565","pred":"pobj","subj":"T6690","obj":"T6688"},{"id":"R5566","pred":"punct","subj":"T6691","obj":"T6693"},{"id":"R5567","pred":"compound","subj":"T6692","obj":"T6693"},{"id":"R5568","pred":"appos","subj":"T6693","obj":"T6690"},{"id":"R5569","pred":"punct","subj":"T6694","obj":"T6690"},{"id":"R5570","pred":"punct","subj":"T6695","obj":"T6674"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

{"project":"GO-BP","denotations":[{"id":"T6892","span":{"begin":49,"end":64},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6893","span":{"begin":226,"end":241},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6894","span":{"begin":415,"end":430},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6895","span":{"begin":492,"end":507},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6896","span":{"begin":635,"end":650},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6897","span":{"begin":824,"end":839},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6898","span":{"begin":1073,"end":1088},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6899","span":{"begin":93,"end":104},"obj":"http://purl.obolibrary.org/obo/GO_0009056"},{"id":"T6900","span":{"begin":985,"end":1014},"obj":"http://purl.obolibrary.org/obo/GO_0043392"},{"id":"T6901","span":{"begin":985,"end":1014},"obj":"http://purl.obolibrary.org/obo/GO_1904743"},{"id":"T6902","span":{"begin":1067,"end":1088},"obj":"http://purl.obolibrary.org/obo/GO_0007252"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

{"project":"GO-MF","denotations":[{"id":"T6924","span":{"begin":875,"end":886},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T6925","span":{"begin":1003,"end":1014},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T6926","span":{"begin":879,"end":886},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T6927","span":{"begin":1007,"end":1014},"obj":"http://purl.obolibrary.org/obo/GO_0005488"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

{"project":"GO-CC","denotations":[{"id":"T6928","span":{"begin":956,"end":961},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

{"project":"sentences","denotations":[{"id":"T6497","span":{"begin":0,"end":42},"obj":"Sentence"},{"id":"T6498","span":{"begin":43,"end":207},"obj":"Sentence"},{"id":"T6499","span":{"begin":208,"end":348},"obj":"Sentence"},{"id":"T6500","span":{"begin":349,"end":451},"obj":"Sentence"},{"id":"T6501","span":{"begin":452,"end":580},"obj":"Sentence"},{"id":"T6502","span":{"begin":581,"end":748},"obj":"Sentence"},{"id":"T6503","span":{"begin":749,"end":905},"obj":"Sentence"},{"id":"T6504","span":{"begin":906,"end":1101},"obj":"Sentence"},{"id":"T109","span":{"begin":0,"end":42},"obj":"Sentence"},{"id":"T110","span":{"begin":43,"end":207},"obj":"Sentence"},{"id":"T111","span":{"begin":208,"end":348},"obj":"Sentence"},{"id":"T112","span":{"begin":349,"end":451},"obj":"Sentence"},{"id":"T113","span":{"begin":452,"end":580},"obj":"Sentence"},{"id":"T114","span":{"begin":581,"end":748},"obj":"Sentence"},{"id":"T115","span":{"begin":749,"end":905},"obj":"Sentence"},{"id":"T116","span":{"begin":906,"end":1101},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

{"project":"events-check-again","denotations":[{"id":"T6929","span":{"begin":65,"end":70},"obj":"Positive_regulation"},{"id":"T6930","span":{"begin":93,"end":104},"obj":"Protein_catabolism"},{"id":"T6931","span":{"begin":108,"end":113},"obj":"Protein"},{"id":"T6932","span":{"begin":183,"end":190},"obj":"Regulation"},{"id":"T6933","span":{"begin":201,"end":206},"obj":"Protein"},{"id":"T6934","span":{"begin":220,"end":225},"obj":"Protein"},{"id":"T6935","span":{"begin":226,"end":241},"obj":"Phosphorylation"},{"id":"T6936","span":{"begin":255,"end":264},"obj":"Positive_regulation"},{"id":"T6937","span":{"begin":265,"end":274},"obj":"Positive_regulation"},{"id":"T6938","span":{"begin":383,"end":396},"obj":"Negative_regulation"},{"id":"T6939","span":{"begin":401,"end":408},"obj":"Positive_regulation"},{"id":"T6940","span":{"begin":409,"end":414},"obj":"Protein"},{"id":"T6941","span":{"begin":415,"end":430},"obj":"Phosphorylation"},{"id":"T6942","span":{"begin":488,"end":491},"obj":"Protein"},{"id":"T6943","span":{"begin":492,"end":507},"obj":"Phosphorylation"},{"id":"T6944","span":{"begin":512,"end":521},"obj":"Positive_regulation"},{"id":"T6945","span":{"begin":522,"end":531},"obj":"Positive_regulation"},{"id":"T6946","span":{"begin":595,"end":604},"obj":"Negative_regulation"},{"id":"T6947","span":{"begin":609,"end":616},"obj":"Positive_regulation"},{"id":"T6948","span":{"begin":623,"end":626},"obj":"Protein"},{"id":"T6949","span":{"begin":635,"end":650},"obj":"Phosphorylation"}],"relations":[{"id":"R5767","pred":"themeOf","subj":"T6930","obj":"T6929"},{"id":"R5768","pred":"themeOf","subj":"T6931","obj":"T6930"},{"id":"R5769","pred":"themeOf","subj":"T6933","obj":"T6932"},{"id":"R5770","pred":"themeOf","subj":"T6934","obj":"T6935"},{"id":"R5771","pred":"themeOf","subj":"T6935","obj":"T6936"},{"id":"R5772","pred":"themeOf","subj":"T6936","obj":"T6937"},{"id":"R5773","pred":"themeOf","subj":"T6939","obj":"T6938"},{"id":"R5774","pred":"themeOf","subj":"T6940","obj":"T6941"},{"id":"R5775","pred":"themeOf","subj":"T6941","obj":"T6939"},{"id":"R5776","pred":"themeOf","subj":"T6942","obj":"T6943"},{"id":"R5777","pred":"themeOf","subj":"T6943","obj":"T6944"},{"id":"R5778","pred":"themeOf","subj":"T6944","obj":"T6945"},{"id":"R5779","pred":"themeOf","subj":"T6947","obj":"T6946"},{"id":"R5780","pred":"themeOf","subj":"T6948","obj":"T6949"},{"id":"R5781","pred":"themeOf","subj":"T6949","obj":"T6947"}],"attributes":[{"id":"M37","pred":"Speculation","subj":"T6932","obj":"true"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T6950","span":{"begin":26,"end":31},"obj":"Protein"},{"id":"T6951","span":{"begin":108,"end":113},"obj":"Protein"},{"id":"T6952","span":{"begin":154,"end":159},"obj":"Protein"},{"id":"T6953","span":{"begin":201,"end":206},"obj":"Protein"},{"id":"T6954","span":{"begin":93,"end":104},"obj":"Protein_catabolism"},{"id":"T6955","span":{"begin":129,"end":136},"obj":"Entity"},{"id":"T6956","span":{"begin":137,"end":150},"obj":"Localization"},{"id":"T6957","span":{"begin":137,"end":150},"obj":"Localization"},{"id":"T6958","span":{"begin":183,"end":190},"obj":"Regulation"},{"id":"T6959","span":{"begin":208,"end":221},"obj":"Protein"},{"id":"T6960","span":{"begin":222,"end":225},"obj":"Protein"},{"id":"T6961","span":{"begin":226,"end":241},"obj":"Phosphorylation"},{"id":"T6962","span":{"begin":226,"end":241},"obj":"Phosphorylation"},{"id":"T6963","span":{"begin":255,"end":264},"obj":"Positive_regulation"},{"id":"T6964","span":{"begin":255,"end":264},"obj":"Positive_regulation"},{"id":"T6965","span":{"begin":365,"end":368},"obj":"Protein"},{"id":"T6966","span":{"begin":409,"end":414},"obj":"Protein"},{"id":"T6967","span":{"begin":415,"end":430},"obj":"Phosphorylation"},{"id":"T6968","span":{"begin":383,"end":396},"obj":"Negative_regulation"},{"id":"T6969","span":{"begin":397,"end":408},"obj":"Positive_regulation"},{"id":"T6970","span":{"begin":383,"end":396},"obj":"Negative_regulation"},{"id":"T6971","span":{"begin":488,"end":491},"obj":"Protein"},{"id":"T6972","span":{"begin":474,"end":481},"obj":"Entity"},{"id":"T6973","span":{"begin":492,"end":507},"obj":"Phosphorylation"},{"id":"T6974","span":{"begin":512,"end":521},"obj":"Positive_regulation"},{"id":"T6975","span":{"begin":617,"end":622},"obj":"Protein"},{"id":"T6976","span":{"begin":623,"end":626},"obj":"Protein"},{"id":"T6977","span":{"begin":664,"end":669},"obj":"Protein"},{"id":"T6978","span":{"begin":627,"end":634},"obj":"Entity"},{"id":"T6979","span":{"begin":635,"end":650},"obj":"Phosphorylation"},{"id":"T6980","span":{"begin":635,"end":650},"obj":"Phosphorylation"},{"id":"T6981","span":{"begin":670,"end":680},"obj":"Positive_regulation"},{"id":"T6982","span":{"begin":693,"end":706},"obj":"Localization"},{"id":"T6983","span":{"begin":693,"end":706},"obj":"Localization"},{"id":"T6984","span":{"begin":605,"end":616},"obj":"Positive_regulation"},{"id":"T6985","span":{"begin":605,"end":616},"obj":"Positive_regulation"},{"id":"T6986","span":{"begin":595,"end":604},"obj":"Negative_regulation"},{"id":"T6987","span":{"begin":595,"end":604},"obj":"Negative_regulation"},{"id":"T6988","span":{"begin":789,"end":792},"obj":"Protein"},{"id":"T6989","span":{"begin":899,"end":904},"obj":"Protein"},{"id":"T6990","span":{"begin":879,"end":886},"obj":"Binding"},{"id":"T6991","span":{"begin":922,"end":925},"obj":"Protein"},{"id":"T6992","span":{"begin":1067,"end":1072},"obj":"Protein"},{"id":"T6993","span":{"begin":1073,"end":1088},"obj":"Phosphorylation"},{"id":"T6994","span":{"begin":1049,"end":1063},"obj":"Negative_regulation"}],"relations":[{"id":"R5782","pred":"themeOf","subj":"T6951","obj":"T6954"},{"id":"R5783","pred":"themeOf","subj":"T6951","obj":"T6956"},{"id":"R5784","pred":"themeOf","subj":"T6952","obj":"T6957"},{"id":"R5785","pred":"themeOf","subj":"T6953","obj":"T6958"},{"id":"R5786","pred":"locationOf","subj":"T6955","obj":"T6956"},{"id":"R5787","pred":"locationOf","subj":"T6955","obj":"T6957"},{"id":"R5788","pred":"themeOf","subj":"T6959","obj":"T6961"},{"id":"R5789","pred":"themeOf","subj":"T6960","obj":"T6962"},{"id":"R5790","pred":"themeOf","subj":"T6961","obj":"T6963"},{"id":"R5791","pred":"themeOf","subj":"T6962","obj":"T6964"},{"id":"R5792","pred":"causeOf","subj":"T6965","obj":"T6968"},{"id":"R5793","pred":"causeOf","subj":"T6965","obj":"T6970"},{"id":"R5794","pred":"themeOf","subj":"T6966","obj":"T6967"},{"id":"R5795","pred":"themeOf","subj":"T6967","obj":"T6968"},{"id":"R5796","pred":"themeOf","subj":"T6967","obj":"T6969"},{"id":"R5797","pred":"themeOf","subj":"T6969","obj":"T6970"},{"id":"R5798","pred":"themeOf","subj":"T6971","obj":"T6973"},{"id":"R5799","pred":"Site","subj":"T6972","obj":"T6973"},{"id":"R5800","pred":"themeOf","subj":"T6973","obj":"T6974"},{"id":"R5801","pred":"themeOf","subj":"T6975","obj":"T6979"},{"id":"R5802","pred":"themeOf","subj":"T6975","obj":"T6982"},{"id":"R5803","pred":"themeOf","subj":"T6976","obj":"T6980"},{"id":"R5804","pred":"themeOf","subj":"T6976","obj":"T6983"},{"id":"R5805","pred":"themeOf","subj":"T6977","obj":"T6981"},{"id":"R5806","pred":"Site","subj":"T6978","obj":"T6979"},{"id":"R5807","pred":"Site","subj":"T6978","obj":"T6980"},{"id":"R5808","pred":"themeOf","subj":"T6979","obj":"T6984"},{"id":"R5809","pred":"themeOf","subj":"T6980","obj":"T6985"},{"id":"R5810","pred":"themeOf","subj":"T6984","obj":"T6986"},{"id":"R5811","pred":"themeOf","subj":"T6985","obj":"T6987"},{"id":"R5812","pred":"themeOf","subj":"T6989","obj":"T6990"},{"id":"R5813","pred":"themeOf","subj":"T6992","obj":"T6993"},{"id":"R5814","pred":"themeOf","subj":"T6993","obj":"T6994"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T6880","span":{"begin":488,"end":491},"obj":"Q04206"},{"id":"T6881","span":{"begin":488,"end":491},"obj":"P21579"},{"id":"T6882","span":{"begin":623,"end":626},"obj":"Q04206"},{"id":"T6883","span":{"begin":623,"end":626},"obj":"P21579"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

{"project":"test2","denotations":[{"id":"T6452","span":{"begin":93,"end":104},"obj":"Protein_catabolism"},{"id":"T6453","span":{"begin":108,"end":113},"obj":"Protein"},{"id":"T6454","span":{"begin":129,"end":136},"obj":"Entity"},{"id":"T6455","span":{"begin":137,"end":150},"obj":"Localization"},{"id":"T6456","span":{"begin":183,"end":190},"obj":"Regulation"},{"id":"T6457","span":{"begin":201,"end":206},"obj":"Protein"},{"id":"T6458","span":{"begin":220,"end":225},"obj":"Protein"},{"id":"T6459","span":{"begin":226,"end":241},"obj":"Phosphorylation"},{"id":"T6460","span":{"begin":255,"end":264},"obj":"Positive_regulation"},{"id":"T6461","span":{"begin":265,"end":274},"obj":"Positive_regulation"},{"id":"T6462","span":{"begin":383,"end":396},"obj":"Negative_regulation"},{"id":"T6463","span":{"begin":401,"end":408},"obj":"Positive_regulation"},{"id":"T6464","span":{"begin":409,"end":414},"obj":"Protein"},{"id":"T6465","span":{"begin":415,"end":430},"obj":"Phosphorylation"},{"id":"T6466","span":{"begin":488,"end":491},"obj":"Protein"},{"id":"T6467","span":{"begin":492,"end":507},"obj":"Phosphorylation"},{"id":"T6468","span":{"begin":512,"end":521},"obj":"Positive_regulation"},{"id":"T6469","span":{"begin":522,"end":531},"obj":"Positive_regulation"},{"id":"T6470","span":{"begin":595,"end":604},"obj":"Negative_regulation"},{"id":"T6471","span":{"begin":609,"end":616},"obj":"Positive_regulation"},{"id":"T6472","span":{"begin":623,"end":626},"obj":"Protein"},{"id":"T6473","span":{"begin":627,"end":634},"obj":"Entity"},{"id":"T6474","span":{"begin":635,"end":650},"obj":"Phosphorylation"},{"id":"T6475","span":{"begin":816,"end":823},"obj":"Entity"}],"relations":[{"id":"R5349","pred":"themeOf","subj":"T6453","obj":"T6452"},{"id":"R5350","pred":"locationOf","subj":"T6454","obj":"T6455"},{"id":"R5351","pred":"themeOf","subj":"T6457","obj":"T6456"},{"id":"R5352","pred":"themeOf","subj":"T6458","obj":"T6459"},{"id":"R5353","pred":"themeOf","subj":"T6459","obj":"T6460"},{"id":"R5354","pred":"themeOf","subj":"T6460","obj":"T6461"},{"id":"R5355","pred":"themeOf","subj":"T6463","obj":"T6462"},{"id":"R5356","pred":"themeOf","subj":"T6464","obj":"T6465"},{"id":"R5357","pred":"themeOf","subj":"T6465","obj":"T6463"},{"id":"R5358","pred":"themeOf","subj":"T6466","obj":"T6467"},{"id":"R5359","pred":"themeOf","subj":"T6467","obj":"T6468"},{"id":"R5360","pred":"themeOf","subj":"T6468","obj":"T6469"},{"id":"R5361","pred":"themeOf","subj":"T6471","obj":"T6470"},{"id":"R5362","pred":"themeOf","subj":"T6472","obj":"T6474"},{"id":"R5363","pred":"locationOf","subj":"T6473","obj":"T6474"},{"id":"R5364","pred":"themeOf","subj":"T6474","obj":"T6471"}],"attributes":[{"id":"M34","pred":"Speculation","subj":"T6454","obj":"true"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}