PMC:2664230 / 14139-15240 JSONTXT

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    bionlp-st-ge-2016-reference

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    pmc-enju-pas

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decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

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decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

    GO-BP

    {"project":"GO-BP","denotations":[{"id":"T6892","span":{"begin":49,"end":64},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6893","span":{"begin":226,"end":241},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6894","span":{"begin":415,"end":430},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6895","span":{"begin":492,"end":507},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6896","span":{"begin":635,"end":650},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6897","span":{"begin":824,"end":839},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6898","span":{"begin":1073,"end":1088},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T6899","span":{"begin":93,"end":104},"obj":"http://purl.obolibrary.org/obo/GO_0009056"},{"id":"T6900","span":{"begin":985,"end":1014},"obj":"http://purl.obolibrary.org/obo/GO_0043392"},{"id":"T6901","span":{"begin":985,"end":1014},"obj":"http://purl.obolibrary.org/obo/GO_1904743"},{"id":"T6902","span":{"begin":1067,"end":1088},"obj":"http://purl.obolibrary.org/obo/GO_0007252"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

    GO-MF

    {"project":"GO-MF","denotations":[{"id":"T6924","span":{"begin":875,"end":886},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T6925","span":{"begin":1003,"end":1014},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T6926","span":{"begin":879,"end":886},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T6927","span":{"begin":1007,"end":1014},"obj":"http://purl.obolibrary.org/obo/GO_0005488"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

    GO-CC

    {"project":"GO-CC","denotations":[{"id":"T6928","span":{"begin":956,"end":961},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

    sentences

    {"project":"sentences","denotations":[{"id":"T6497","span":{"begin":0,"end":42},"obj":"Sentence"},{"id":"T6498","span":{"begin":43,"end":207},"obj":"Sentence"},{"id":"T6499","span":{"begin":208,"end":348},"obj":"Sentence"},{"id":"T6500","span":{"begin":349,"end":451},"obj":"Sentence"},{"id":"T6501","span":{"begin":452,"end":580},"obj":"Sentence"},{"id":"T6502","span":{"begin":581,"end":748},"obj":"Sentence"},{"id":"T6503","span":{"begin":749,"end":905},"obj":"Sentence"},{"id":"T6504","span":{"begin":906,"end":1101},"obj":"Sentence"},{"id":"T109","span":{"begin":0,"end":42},"obj":"Sentence"},{"id":"T110","span":{"begin":43,"end":207},"obj":"Sentence"},{"id":"T111","span":{"begin":208,"end":348},"obj":"Sentence"},{"id":"T112","span":{"begin":349,"end":451},"obj":"Sentence"},{"id":"T113","span":{"begin":452,"end":580},"obj":"Sentence"},{"id":"T114","span":{"begin":581,"end":748},"obj":"Sentence"},{"id":"T115","span":{"begin":749,"end":905},"obj":"Sentence"},{"id":"T116","span":{"begin":906,"end":1101},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

    events-check-again

    {"project":"events-check-again","denotations":[{"id":"T6929","span":{"begin":65,"end":70},"obj":"Positive_regulation"},{"id":"T6930","span":{"begin":93,"end":104},"obj":"Protein_catabolism"},{"id":"T6931","span":{"begin":108,"end":113},"obj":"Protein"},{"id":"T6932","span":{"begin":183,"end":190},"obj":"Regulation"},{"id":"T6933","span":{"begin":201,"end":206},"obj":"Protein"},{"id":"T6934","span":{"begin":220,"end":225},"obj":"Protein"},{"id":"T6935","span":{"begin":226,"end":241},"obj":"Phosphorylation"},{"id":"T6936","span":{"begin":255,"end":264},"obj":"Positive_regulation"},{"id":"T6937","span":{"begin":265,"end":274},"obj":"Positive_regulation"},{"id":"T6938","span":{"begin":383,"end":396},"obj":"Negative_regulation"},{"id":"T6939","span":{"begin":401,"end":408},"obj":"Positive_regulation"},{"id":"T6940","span":{"begin":409,"end":414},"obj":"Protein"},{"id":"T6941","span":{"begin":415,"end":430},"obj":"Phosphorylation"},{"id":"T6942","span":{"begin":488,"end":491},"obj":"Protein"},{"id":"T6943","span":{"begin":492,"end":507},"obj":"Phosphorylation"},{"id":"T6944","span":{"begin":512,"end":521},"obj":"Positive_regulation"},{"id":"T6945","span":{"begin":522,"end":531},"obj":"Positive_regulation"},{"id":"T6946","span":{"begin":595,"end":604},"obj":"Negative_regulation"},{"id":"T6947","span":{"begin":609,"end":616},"obj":"Positive_regulation"},{"id":"T6948","span":{"begin":623,"end":626},"obj":"Protein"},{"id":"T6949","span":{"begin":635,"end":650},"obj":"Phosphorylation"}],"relations":[{"id":"R5767","pred":"themeOf","subj":"T6930","obj":"T6929"},{"id":"R5768","pred":"themeOf","subj":"T6931","obj":"T6930"},{"id":"R5769","pred":"themeOf","subj":"T6933","obj":"T6932"},{"id":"R5770","pred":"themeOf","subj":"T6934","obj":"T6935"},{"id":"R5771","pred":"themeOf","subj":"T6935","obj":"T6936"},{"id":"R5772","pred":"themeOf","subj":"T6936","obj":"T6937"},{"id":"R5773","pred":"themeOf","subj":"T6939","obj":"T6938"},{"id":"R5774","pred":"themeOf","subj":"T6940","obj":"T6941"},{"id":"R5775","pred":"themeOf","subj":"T6941","obj":"T6939"},{"id":"R5776","pred":"themeOf","subj":"T6942","obj":"T6943"},{"id":"R5777","pred":"themeOf","subj":"T6943","obj":"T6944"},{"id":"R5778","pred":"themeOf","subj":"T6944","obj":"T6945"},{"id":"R5779","pred":"themeOf","subj":"T6947","obj":"T6946"},{"id":"R5780","pred":"themeOf","subj":"T6948","obj":"T6949"},{"id":"R5781","pred":"themeOf","subj":"T6949","obj":"T6947"}],"attributes":[{"id":"M37","pred":"Speculation","subj":"T6932","obj":"true"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

    bionlp-st-ge-2016-reference-tees

    {"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T6950","span":{"begin":26,"end":31},"obj":"Protein"},{"id":"T6951","span":{"begin":108,"end":113},"obj":"Protein"},{"id":"T6952","span":{"begin":154,"end":159},"obj":"Protein"},{"id":"T6953","span":{"begin":201,"end":206},"obj":"Protein"},{"id":"T6954","span":{"begin":93,"end":104},"obj":"Protein_catabolism"},{"id":"T6955","span":{"begin":129,"end":136},"obj":"Entity"},{"id":"T6956","span":{"begin":137,"end":150},"obj":"Localization"},{"id":"T6957","span":{"begin":137,"end":150},"obj":"Localization"},{"id":"T6958","span":{"begin":183,"end":190},"obj":"Regulation"},{"id":"T6959","span":{"begin":208,"end":221},"obj":"Protein"},{"id":"T6960","span":{"begin":222,"end":225},"obj":"Protein"},{"id":"T6961","span":{"begin":226,"end":241},"obj":"Phosphorylation"},{"id":"T6962","span":{"begin":226,"end":241},"obj":"Phosphorylation"},{"id":"T6963","span":{"begin":255,"end":264},"obj":"Positive_regulation"},{"id":"T6964","span":{"begin":255,"end":264},"obj":"Positive_regulation"},{"id":"T6965","span":{"begin":365,"end":368},"obj":"Protein"},{"id":"T6966","span":{"begin":409,"end":414},"obj":"Protein"},{"id":"T6967","span":{"begin":415,"end":430},"obj":"Phosphorylation"},{"id":"T6968","span":{"begin":383,"end":396},"obj":"Negative_regulation"},{"id":"T6969","span":{"begin":397,"end":408},"obj":"Positive_regulation"},{"id":"T6970","span":{"begin":383,"end":396},"obj":"Negative_regulation"},{"id":"T6971","span":{"begin":488,"end":491},"obj":"Protein"},{"id":"T6972","span":{"begin":474,"end":481},"obj":"Entity"},{"id":"T6973","span":{"begin":492,"end":507},"obj":"Phosphorylation"},{"id":"T6974","span":{"begin":512,"end":521},"obj":"Positive_regulation"},{"id":"T6975","span":{"begin":617,"end":622},"obj":"Protein"},{"id":"T6976","span":{"begin":623,"end":626},"obj":"Protein"},{"id":"T6977","span":{"begin":664,"end":669},"obj":"Protein"},{"id":"T6978","span":{"begin":627,"end":634},"obj":"Entity"},{"id":"T6979","span":{"begin":635,"end":650},"obj":"Phosphorylation"},{"id":"T6980","span":{"begin":635,"end":650},"obj":"Phosphorylation"},{"id":"T6981","span":{"begin":670,"end":680},"obj":"Positive_regulation"},{"id":"T6982","span":{"begin":693,"end":706},"obj":"Localization"},{"id":"T6983","span":{"begin":693,"end":706},"obj":"Localization"},{"id":"T6984","span":{"begin":605,"end":616},"obj":"Positive_regulation"},{"id":"T6985","span":{"begin":605,"end":616},"obj":"Positive_regulation"},{"id":"T6986","span":{"begin":595,"end":604},"obj":"Negative_regulation"},{"id":"T6987","span":{"begin":595,"end":604},"obj":"Negative_regulation"},{"id":"T6988","span":{"begin":789,"end":792},"obj":"Protein"},{"id":"T6989","span":{"begin":899,"end":904},"obj":"Protein"},{"id":"T6990","span":{"begin":879,"end":886},"obj":"Binding"},{"id":"T6991","span":{"begin":922,"end":925},"obj":"Protein"},{"id":"T6992","span":{"begin":1067,"end":1072},"obj":"Protein"},{"id":"T6993","span":{"begin":1073,"end":1088},"obj":"Phosphorylation"},{"id":"T6994","span":{"begin":1049,"end":1063},"obj":"Negative_regulation"}],"relations":[{"id":"R5782","pred":"themeOf","subj":"T6951","obj":"T6954"},{"id":"R5783","pred":"themeOf","subj":"T6951","obj":"T6956"},{"id":"R5784","pred":"themeOf","subj":"T6952","obj":"T6957"},{"id":"R5785","pred":"themeOf","subj":"T6953","obj":"T6958"},{"id":"R5786","pred":"locationOf","subj":"T6955","obj":"T6956"},{"id":"R5787","pred":"locationOf","subj":"T6955","obj":"T6957"},{"id":"R5788","pred":"themeOf","subj":"T6959","obj":"T6961"},{"id":"R5789","pred":"themeOf","subj":"T6960","obj":"T6962"},{"id":"R5790","pred":"themeOf","subj":"T6961","obj":"T6963"},{"id":"R5791","pred":"themeOf","subj":"T6962","obj":"T6964"},{"id":"R5792","pred":"causeOf","subj":"T6965","obj":"T6968"},{"id":"R5793","pred":"causeOf","subj":"T6965","obj":"T6970"},{"id":"R5794","pred":"themeOf","subj":"T6966","obj":"T6967"},{"id":"R5795","pred":"themeOf","subj":"T6967","obj":"T6968"},{"id":"R5796","pred":"themeOf","subj":"T6967","obj":"T6969"},{"id":"R5797","pred":"themeOf","subj":"T6969","obj":"T6970"},{"id":"R5798","pred":"themeOf","subj":"T6971","obj":"T6973"},{"id":"R5799","pred":"Site","subj":"T6972","obj":"T6973"},{"id":"R5800","pred":"themeOf","subj":"T6973","obj":"T6974"},{"id":"R5801","pred":"themeOf","subj":"T6975","obj":"T6979"},{"id":"R5802","pred":"themeOf","subj":"T6975","obj":"T6982"},{"id":"R5803","pred":"themeOf","subj":"T6976","obj":"T6980"},{"id":"R5804","pred":"themeOf","subj":"T6976","obj":"T6983"},{"id":"R5805","pred":"themeOf","subj":"T6977","obj":"T6981"},{"id":"R5806","pred":"Site","subj":"T6978","obj":"T6979"},{"id":"R5807","pred":"Site","subj":"T6978","obj":"T6980"},{"id":"R5808","pred":"themeOf","subj":"T6979","obj":"T6984"},{"id":"R5809","pred":"themeOf","subj":"T6980","obj":"T6985"},{"id":"R5810","pred":"themeOf","subj":"T6984","obj":"T6986"},{"id":"R5811","pred":"themeOf","subj":"T6985","obj":"T6987"},{"id":"R5812","pred":"themeOf","subj":"T6989","obj":"T6990"},{"id":"R5813","pred":"themeOf","subj":"T6992","obj":"T6993"},{"id":"R5814","pred":"themeOf","subj":"T6993","obj":"T6994"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

    bionlp-st-ge-2016-uniprot

    {"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T6880","span":{"begin":488,"end":491},"obj":"Q04206"},{"id":"T6881","span":{"begin":488,"end":491},"obj":"P21579"},{"id":"T6882","span":{"begin":623,"end":626},"obj":"Q04206"},{"id":"T6883","span":{"begin":623,"end":626},"obj":"P21579"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}

    test2

    {"project":"test2","denotations":[{"id":"T6452","span":{"begin":93,"end":104},"obj":"Protein_catabolism"},{"id":"T6453","span":{"begin":108,"end":113},"obj":"Protein"},{"id":"T6454","span":{"begin":129,"end":136},"obj":"Entity"},{"id":"T6455","span":{"begin":137,"end":150},"obj":"Localization"},{"id":"T6456","span":{"begin":183,"end":190},"obj":"Regulation"},{"id":"T6457","span":{"begin":201,"end":206},"obj":"Protein"},{"id":"T6458","span":{"begin":220,"end":225},"obj":"Protein"},{"id":"T6459","span":{"begin":226,"end":241},"obj":"Phosphorylation"},{"id":"T6460","span":{"begin":255,"end":264},"obj":"Positive_regulation"},{"id":"T6461","span":{"begin":265,"end":274},"obj":"Positive_regulation"},{"id":"T6462","span":{"begin":383,"end":396},"obj":"Negative_regulation"},{"id":"T6463","span":{"begin":401,"end":408},"obj":"Positive_regulation"},{"id":"T6464","span":{"begin":409,"end":414},"obj":"Protein"},{"id":"T6465","span":{"begin":415,"end":430},"obj":"Phosphorylation"},{"id":"T6466","span":{"begin":488,"end":491},"obj":"Protein"},{"id":"T6467","span":{"begin":492,"end":507},"obj":"Phosphorylation"},{"id":"T6468","span":{"begin":512,"end":521},"obj":"Positive_regulation"},{"id":"T6469","span":{"begin":522,"end":531},"obj":"Positive_regulation"},{"id":"T6470","span":{"begin":595,"end":604},"obj":"Negative_regulation"},{"id":"T6471","span":{"begin":609,"end":616},"obj":"Positive_regulation"},{"id":"T6472","span":{"begin":623,"end":626},"obj":"Protein"},{"id":"T6473","span":{"begin":627,"end":634},"obj":"Entity"},{"id":"T6474","span":{"begin":635,"end":650},"obj":"Phosphorylation"},{"id":"T6475","span":{"begin":816,"end":823},"obj":"Entity"}],"relations":[{"id":"R5349","pred":"themeOf","subj":"T6453","obj":"T6452"},{"id":"R5350","pred":"locationOf","subj":"T6454","obj":"T6455"},{"id":"R5351","pred":"themeOf","subj":"T6457","obj":"T6456"},{"id":"R5352","pred":"themeOf","subj":"T6458","obj":"T6459"},{"id":"R5353","pred":"themeOf","subj":"T6459","obj":"T6460"},{"id":"R5354","pred":"themeOf","subj":"T6460","obj":"T6461"},{"id":"R5355","pred":"themeOf","subj":"T6463","obj":"T6462"},{"id":"R5356","pred":"themeOf","subj":"T6464","obj":"T6465"},{"id":"R5357","pred":"themeOf","subj":"T6465","obj":"T6463"},{"id":"R5358","pred":"themeOf","subj":"T6466","obj":"T6467"},{"id":"R5359","pred":"themeOf","subj":"T6467","obj":"T6468"},{"id":"R5360","pred":"themeOf","subj":"T6468","obj":"T6469"},{"id":"R5361","pred":"themeOf","subj":"T6471","obj":"T6470"},{"id":"R5362","pred":"themeOf","subj":"T6472","obj":"T6474"},{"id":"R5363","pred":"locationOf","subj":"T6473","obj":"T6474"},{"id":"R5364","pred":"themeOf","subj":"T6474","obj":"T6471"}],"attributes":[{"id":"M34","pred":"Speculation","subj":"T6454","obj":"true"}],"text":"PTX decreases LPS-induced NF-κB activation\nSince phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα. Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P \u003c 0.05). The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)\nIn a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P \u003c 0.01). PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).\nEMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB. The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B)."}