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bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Negation Speculation
T64 583-589 Regulation denotes alters
T65 590-595 Protein denotes TNF-α
T66 596-606 Gene_expression denotes production
T67 607-625 Positive_regulation denotes in the presence of
T68 839-853 Phosphorylation denotes phosphorylated
T69 866-871 Protein denotes I-κBα
T70 891-894 Protein denotes p65
T71 1060-1065 Protein denotes TNF-α
T72 1280-1286 Negative_regulation denotes reduce
T73 1280-1286 Negative_regulation denotes reduce
T74 1299-1304 Protein denotes I-κBα
T75 1305-1320 Phosphorylation denotes phosphorylation
T76 1330-1333 Protein denotes p65
T77 1334-1349 Phosphorylation denotes phosphorylation
T78 1524-1532 Localization denotes secreted
T79 1551-1556 Protein denotes TNF-α
T80 1564-1572 Regulation denotes presence
T81 1577-1584 Regulation denotes absence true
T1133 2747-2757 Negative_regulation denotes attenuated
T1134 2952-2956 Positive_regulation denotes lead
T1135 2964-2978 Negative_regulation denotes downregulation
T1136 2982-2987 Protein denotes TNF-α
T1137 2988-2998 Gene_expression denotes production
T1138 4026-4033 Negative_regulation denotes inhibit
T1139 4040-4049 Regulation denotes dependent
T1140 4072-4085 Transcription denotes transcription
T1141 4107-4112 Protein denotes TNF-α
T1142 4196-4210 Negative_regulation denotes down-regulates
T1143 4211-4216 Protein denotes TNF-α
T1144 4217-4227 Gene_expression denotes production
T1145 4875-4879 Regulation denotes role true
T1146 4894-4901 Positive_regulation denotes induced
T1147 4902-4907 Protein denotes TNF-α
T1148 4908-4918 Gene_expression denotes production
T2441 5667-5681 Phosphorylation denotes phosphorylated
T2442 5682-5686 Protein denotes CREB
T2443 5688-5698 Entity denotes serine 133
T2444 5701-5715 Phosphorylation denotes phosphorylated
T2445 5701-5715 Phosphorylation denotes phosphorylated
T2446 5716-5721 Protein denotes I-κBα
T2447 5723-5733 Entity denotes serines 32
T2448 5738-5740 Entity denotes 36
T2449 5743-5757 Phosphorylation denotes phosphorylated
T2450 5764-5767 Protein denotes p65
T2451 5777-5787 Entity denotes serine 276
T2452 6012-6017 Protein denotes TNF-α
T3248 8217-8224 Positive_regulation denotes induced
T3249 8225-8230 Protein denotes TNF-α
T3250 8231-8241 Gene_expression denotes production
T3251 8394-8407 Positive_regulation denotes up-regulation
T3252 8412-8427 Negative_regulation denotes down-regulation
T3253 8431-8436 Protein denotes TNF-α
T3254 8437-8447 Gene_expression denotes production
T4131 8840-8845 Protein denotes I-κBα
T4132 8853-8856 Protein denotes p65
T4133 8862-8866 Protein denotes CREB
T4134 9829-9843 Phosphorylation denotes phosphorylated
T4135 9844-9849 Protein denotes I-κBα
T4136 9918-9932 Phosphorylation denotes phosphorylated
T4137 9939-9942 Protein denotes p65
T5627 12620-12625 Protein denotes TNF-α
T5628 12626-12636 Gene_expression denotes production
T5629 12666-12677 Regulation denotes involvement
T5630 12685-12696 Negative_regulation denotes attenuation
T5631 12704-12711 Positive_regulation denotes induced
T5632 12712-12717 Protein denotes TNF-α
T5633 12718-12728 Gene_expression denotes production
T5634 13304-13309 Protein denotes TNF-α
T6229 13859-13866 Regulation denotes effects true
T6230 13881-13888 Positive_regulation denotes induced
T6231 13889-13894 Protein denotes TNF-α
T6232 13895-13905 Gene_expression denotes production
T6233 13953-13958 Protein denotes TNF-α
T6234 13959-13969 Gene_expression denotes production
T6235 13980-13991 Positive_regulation denotes stimulation
T6236 14090-14096 Regulation denotes effect
T6237 14100-14105 Protein denotes TNF-α
T6238 14106-14116 Gene_expression denotes production
T6483 14394-14403 Positive_regulation denotes increased
T6484 14404-14413 Positive_regulation denotes following
T6485 14522-14535 Negative_regulation denotes downregulated
T6486 14540-14547 Positive_regulation denotes induced
T6487 14548-14553 Protein denotes I-κBα
T6488 14554-14569 Phosphorylation denotes phosphorylation
T6489 14627-14630 Protein denotes p65
T6490 14631-14646 Phosphorylation denotes phosphorylation
T6491 14651-14660 Positive_regulation denotes increased
T6492 14661-14670 Positive_regulation denotes following
T6493 14734-14743 Negative_regulation denotes decreased
T6494 14748-14755 Positive_regulation denotes induced
T6495 14762-14765 Protein denotes p65
T6496 14774-14789 Phosphorylation denotes phosphorylation
T7586 16177-16183 Regulation denotes Effect true
T7587 16194-16199 Protein denotes TNF-α
T7588 16200-16210 Gene_expression denotes production
T7589 16211-16229 Regulation denotes in the presence of
T7590 16249-16253 Regulation denotes role
T7591 16268-16275 Positive_regulation denotes induced
T7592 16276-16290 Negative_regulation denotes downregulation
T7593 16294-16299 Protein denotes TNF-α
T7594 16300-16310 Gene_expression denotes production
T7595 16489-16497 Positive_regulation denotes resulted
T7596 16503-16512 Negative_regulation denotes reduction
T7597 16516-16521 Protein denotes TNF-α
T7598 16694-16700 Regulation denotes result true
T7599 16720-16730 Regulation denotes modulation
T7600 16734-16739 Protein denotes TNF-α
T7601 16740-16750 Gene_expression denotes production
T7602 16849-16863 Negative_regulation denotes downregulation
T7603 16867-16872 Protein denotes TNF-α
T7604 16873-16883 Gene_expression denotes production
T7605 16953-16964 Regulation denotes independent true
T8293 18093-18104 Negative_regulation denotes attenuation
T8294 18112-18119 Positive_regulation denotes induced
T8295 18120-18125 Protein denotes TNF-α
T8296 18126-18136 Gene_expression denotes expression
T8297 18301-18310 Negative_regulation denotes decreases true
T8298 18311-18316 Protein denotes TNF-α
T8299 18317-18327 Gene_expression denotes production
T8300 18774-18785 Negative_regulation denotes attenuation
T8301 18789-18794 Protein denotes TNF-α
T8302 18795-18805 Gene_expression denotes production
T8303 18848-18857 Positive_regulation denotes following
T8304 18878-18889 Regulation denotes independent true
T8305 19305-19312 Regulation denotes effects
T8306 19316-19321 Protein denotes TNF-α
T8307 19322-19331 Gene_expression denotes synthesis
T8308 19409-19423 Negative_regulation denotes downregulation
T8309 19427-19432 Protein denotes TNF-α
T8310 20038-20041 Protein denotes p50
T8311 20042-20045 Protein denotes p65
T8312 20154-20159 Protein denotes I-κBα
T8313 20322-20327 Protein denotes IKK-1
T8314 20332-20337 Protein denotes IKK-2
T8315 20353-20367 Phosphorylation denotes phosphorylates
T8316 20372-20377 Protein denotes I-κBα
T8317 20387-20402 Phosphorylation denotes Phosphorylation
T8318 20404-20418 Ubiquitination denotes ubiquitination
T8319 20435-20446 Protein_catabolism denotes degradation
T8320 20450-20455 Protein denotes I-κBα
T8321 20716-20721 Protein denotes TNF-α
T8322 20788-20796 Regulation denotes involved true
T8323 20800-20805 Protein denotes TNF-α
T8324 20806-20821 Gene_expression denotes gene expression
T8325 20903-20916 Negative_regulation denotes downregulates
T8326 20903-20916 Negative_regulation denotes downregulates
T8327 20903-20916 Negative_regulation denotes downregulates
T8328 20929-20934 Protein denotes I-κBα
T8329 20935-20950 Phosphorylation denotes phosphorylation
T8330 20966-20969 Protein denotes p65
T8331 20970-20985 Phosphorylation denotes phosphorylation
T8332 20986-20999 Localization denotes translocation
T8333 21124-21129 Protein denotes I-κBα
T8334 21130-21145 Phosphorylation denotes phosphorylation
T8335 21188-21195 Regulation denotes effects
T8336 21223-21228 Protein denotes I-κBα
T8337 21341-21348 Regulation denotes effects
T8338 21359-21364 Protein denotes I-κBα
T8339 21478-21488 Regulation denotes stabilizes
T8340 21493-21504 Binding denotes interaction
T8341 21513-21518 Protein denotes I-κBα
T8342 21623-21628 Protein denotes I-κBα
T8343 21629-21640 Protein_catabolism denotes degradation
T8344 21672-21680 Regulation denotes presence
T8345 21685-21692 Regulation denotes absence true
T8346 21904-21907 Protein denotes p65
T8347 22428-22439 Negative_regulation denotes attenuation
T8348 22447-22454 Positive_regulation denotes induced
T8349 22455-22460 Protein denotes TNF-α
T8350 22461-22471 Gene_expression denotes production
T8351 22486-22505 Regulation denotes presence or absence true
T8352 23503-23506 Protein denotes CBP
T8353 23525-23529 Protein denotes p300
T8354 23778-23783 Protein denotes c-fos
T8355 23789-23794 Protein denotes c-jun
T8356 23836-23846 Positive_regulation denotes Activation
T8357 23836-23846 Positive_regulation denotes Activation
T8358 23875-23880 Protein denotes c-fos
T8359 23885-23890 Protein denotes c-jun
T8360 23950-23960 Regulation denotes implicated
T8361 23964-23969 Protein denotes TNF-α
T8362 23970-23980 Gene_expression denotes expression
T8363 24006-24015 Binding denotes recognize
T8364 24026-24046 Entity denotes DNA binding sequence
T8365 24077-24082 Protein denotes TNF-α
T8366 24254-24264 Negative_regulation denotes inhibiting
T8367 24265-24270 Protein denotes TNF-α
T8368 24276-24289 Transcription denotes transcription
T8369 24652-24665 Negative_regulation denotes downregulates
T8370 24666-24671 Protein denotes TNF-α
T8371 24672-24682 Gene_expression denotes production
T8372 24716-24727 Regulation denotes independent true
T8373 25018-25023 Protein denotes TNF-α
T8374 25024-25034 Gene_expression denotes production
T54 18-28 Regulation denotes Regulation
T55 32-37 Protein denotes TNF-α
T56 38-48 Gene_expression denotes Production
T57 204-210 Regulation denotes effect true
T58 294-321 Protein denotes tumor necrosis factor-alpha
T59 323-328 Protein denotes TNF-α
T60 330-340 Gene_expression denotes production
T61 423-433 Gene_expression denotes production
T62 437-442 Protein denotes TNF-α
T63 443-452 Positive_regulation denotes following
T8276 17198-17203 Protein denotes TNF-α
T8277 17207-17215 Localization denotes released
T8278 17227-17236 Positive_regulation denotes following
T8279 17428-17437 Positive_regulation denotes increased
T8280 17438-17443 Protein denotes TNF-α
T8281 17444-17453 Gene_expression denotes synthesis
T8282 17673-17683 Negative_regulation denotes decreasing
T8283 17684-17689 Protein denotes TNF-α
T8284 17700-17711 Negative_regulation denotes attenuating
T8285 17716-17726 Gene_expression denotes production
T8286 17778-17790 Negative_regulation denotes downregulate
T8287 17791-17796 Protein denotes TNF-α
T8288 17797-17807 Gene_expression denotes production
T8289 17887-17892 Protein denotes TNF-α
T8290 17912-17920 Positive_regulation denotes increase
T8291 18015-18023 Negative_regulation denotes decrease
T8292 18024-18029 Protein denotes TNF-α
T3964 8526-8533 Positive_regulation denotes induced
T3965 8534-8539 Protein denotes TNF-α
T3966 8540-8550 Gene_expression denotes production
T3967 8593-8598 Protein denotes TNF-α
T5622 12528-12532 Regulation denotes Role
T5623 12547-12554 Positive_regulation denotes induced
T5624 12572-12577 Protein denotes TNF-α
T5625 12578-12588 Gene_expression denotes production
T5626 12605-12609 Regulation denotes role
T6476 14204-14209 Positive_regulation denotes leads
T6477 14232-14243 Protein_catabolism denotes degradation
T6478 14247-14252 Protein denotes I-κBα
T6479 14322-14329 Regulation denotes effects true
T6480 14340-14345 Protein denotes I-κBα
T6481 14359-14364 Protein denotes I-κBα
T6482 14365-14380 Phosphorylation denotes phosphorylation
T7080 16114-16123 Positive_regulation denotes increased
T7081 16124-16128 Protein denotes CREB
T7082 16133-16140 Binding denotes binding
T1126 2558-2567 Regulation denotes regulator
T1127 2571-2594 Protein denotes tumor necrosis factor-α
T1128 2596-2601 Protein denotes TNF-α
T1129 2603-2613 Gene_expression denotes production
T1130 2616-2621 Protein denotes TNF-α
T1131 2724-2729 Protein denotes TNF-α
T1132 2730-2739 Gene_expression denotes synthesis
R1883 T2443 T2441 themeOf serine 133,phosphorylated
R1884 T2443 T2442 partOf serine 133,CREB
R1885 T2447 T2445 themeOf serines 32,phosphorylated
R1886 T2447 T2446 partOf serines 32,I-κBα
R1887 T2448 T2444 themeOf 36,phosphorylated
R1888 T2448 T2446 partOf 36,I-κBα
R1889 T2451 T2449 themeOf serine 276,phosphorylated
R1890 T2451 T2450 partOf serine 276,p65
R4667 T5623 T5622 themeOf induced,Role
R4668 T5624 T5625 themeOf TNF-α,production
R4669 T5625 T5623 themeOf production,induced
R4670 T5627 T5628 themeOf TNF-α,production
R4671 T5628 T5626 themeOf production,role
R4672 T5630 T5629 themeOf attenuation,involvement
R4673 T5631 T5630 themeOf induced,attenuation
R4674 T5632 T5633 themeOf TNF-α,production
R4675 T5633 T5631 themeOf production,induced
R5365 T6477 T6476 themeOf degradation,leads
R5367 T6480 T6479 themeOf I-κBα,effects
R5368 T6481 T6482 themeOf I-κBα,phosphorylation
R5369 T6482 T6483 themeOf phosphorylation,increased
R5370 T6483 T6484 themeOf increased,following
R5371 T6486 T6485 themeOf induced,downregulated
R5372 T6487 T6488 themeOf I-κBα,phosphorylation
R5373 T6488 T6486 themeOf phosphorylation,induced
R5374 T6489 T6490 themeOf p65,phosphorylation
R5375 T6490 T6491 themeOf phosphorylation,increased
R5376 T6491 T6492 themeOf increased,following
R5377 T6494 T6493 themeOf induced,decreased
R5378 T6495 T6496 themeOf p65,phosphorylation
R5379 T6496 T6494 themeOf phosphorylation,induced
R6258 T7586 T7589 themeOf Effect,in the presence of
R6259 T7587 T7588 themeOf TNF-α,production
R6260 T7588 T7586 themeOf production,Effect
R6261 T7591 T7590 themeOf induced,role
R6262 T7592 T7591 themeOf downregulation,induced
R6263 T7593 T7594 themeOf TNF-α,production
R6264 T7594 T7592 themeOf production,downregulation
R6265 T7596 T7595 themeOf reduction,resulted
R6266 T7597 T7596 themeOf TNF-α,reduction
R6267 T7599 T7598 themeOf modulation,result
R6268 T7600 T7601 themeOf TNF-α,production
R6269 T7601 T7599 themeOf production,modulation
R6270 T7602 T7605 themeOf downregulation,independent
R6271 T7603 T7604 themeOf TNF-α,production
R6272 T7604 T7602 themeOf production,downregulation
R6789 T8300 T8303 themeOf attenuation,following
R6790 T8300 T8304 themeOf attenuation,independent
R6791 T8301 T8302 themeOf TNF-α,production
R6792 T8302 T8300 themeOf production,attenuation
R6793 T8306 T8307 themeOf TNF-α,synthesis
R6794 T8307 T8305 themeOf synthesis,effects
R6795 T8309 T8308 themeOf TNF-α,downregulation
R6796 T8316 T8315 themeOf I-κBα,phosphorylates
R6797 T8320 T8317 themeOf I-κBα,Phosphorylation
R6798 T8320 T8318 themeOf I-κBα,ubiquitination
R6799 T8320 T8319 themeOf I-κBα,degradation
R6800 T8323 T8324 themeOf TNF-α,gene expression
R6801 T8324 T8322 themeOf gene expression,involved
R6802 T8328 T8329 themeOf I-κBα,phosphorylation
R6803 T8329 T8326 themeOf phosphorylation,downregulates
R6804 T8330 T8331 themeOf p65,phosphorylation
R6805 T8330 T8332 themeOf p65,translocation
R6806 T8331 T8325 themeOf phosphorylation,downregulates
R6807 T8332 T8327 themeOf translocation,downregulates
R6808 T8333 T8334 themeOf I-κBα,phosphorylation
R6809 T8336 T8335 themeOf I-κBα,effects
R6812 T8341 T8340 themeOf I-κBα,interaction
R6813 T8342 T8343 themeOf I-κBα,degradation
R6814 T8343 T8344 themeOf degradation,presence
R6815 T8343 T8345 themeOf degradation,absence
R6816 T8347 T8351 themeOf attenuation,presence or absence
R6817 T8348 T8347 themeOf induced,attenuation
R6818 T8349 T8350 themeOf TNF-α,production
R6819 T8350 T8348 themeOf production,induced
R6820 T8358 T8356 themeOf c-fos,Activation
R6821 T8359 T8357 themeOf c-jun,Activation
R6822 T8361 T8362 themeOf TNF-α,expression
R6823 T8362 T8360 themeOf expression,implicated
R6824 T8364 T8363 themeOf DNA binding sequence,recognize
R6825 T8364 T8365 partOf DNA binding sequence,TNF-α
R6826 T8367 T8368 themeOf TNF-α,transcription
R6827 T8368 T8366 themeOf transcription,inhibiting
R6828 T8369 T8372 themeOf downregulates,independent
R6829 T8370 T8371 themeOf TNF-α,production
R6830 T8371 T8369 themeOf production,downregulates
R6831 T8373 T8374 themeOf TNF-α,production
R3371 T4135 T4134 themeOf I-κBα,phosphorylated
R3372 T4137 T4136 themeOf p65,phosphorylated
R33 T64 T67 themeOf alters,in the presence of
R34 T65 T66 themeOf TNF-α,production
R35 T66 T64 themeOf production,alters
R36 T69 T68 themeOf I-κBα,phosphorylated
R37 T74 T75 themeOf I-κBα,phosphorylation
R38 T75 T73 themeOf phosphorylation,reduce
R39 T76 T77 themeOf p65,phosphorylation
R40 T77 T72 themeOf phosphorylation,reduce
R41 T78 T80 themeOf secreted,presence
R42 T78 T81 themeOf secreted,absence
R43 T79 T78 themeOf TNF-α,secreted
R748 T55 T56 themeOf TNF-α,Production
R749 T56 T54 themeOf Production,Regulation
R750 T58 T60 themeOf tumor necrosis factor-alpha,production
R751 T60 T57 themeOf production,effect
R752 T61 T63 themeOf production,following
R753 T62 T61 themeOf TNF-α,production
R6777 T8283 T8285 themeOf TNF-α,production
R6779 T8285 T8284 themeOf production,attenuating
R6780 T8287 T8288 themeOf TNF-α,production
R6781 T8288 T8286 themeOf production,downregulate
R6782 T8289 T8290 themeOf TNF-α,increase
R6783 T8292 T8291 themeOf TNF-α,decrease
R6784 T8294 T8293 themeOf induced,attenuation
R6785 T8295 T8296 themeOf TNF-α,expression
R6786 T8296 T8294 themeOf expression,induced
R6787 T8298 T8299 themeOf TNF-α,production
R6788 T8299 T8297 themeOf production,decreases
R6773 T8276 T8277 themeOf TNF-α,released
R6774 T8277 T8278 themeOf released,following
R6775 T8280 T8281 themeOf TNF-α,synthesis
R6776 T8281 T8279 themeOf synthesis,increased
R6778 T8283 T8282 themeOf TNF-α,decreasing
R6810 T8338 T8337 themeOf I-κBα,effects
R6811 T8340 T8339 themeOf interaction,stabilizes
R2595 T3249 T3250 themeOf TNF-α,production
R2596 T3250 T3248 themeOf production,induced
R2597 T3253 T3254 themeOf TNF-α,production
R2598 T3254 T3251 themeOf production,up-regulation
R2599 T3254 T3252 themeOf production,down-regulation
R5179 T6230 T6229 themeOf induced,effects
R5180 T6231 T6232 themeOf TNF-α,production
R5181 T6232 T6230 themeOf production,induced
R5182 T6233 T6234 themeOf TNF-α,production
R5183 T6234 T6235 themeOf production,stimulation
R5184 T6237 T6238 themeOf TNF-α,production
R5185 T6238 T6236 themeOf production,effect
R3248 T3965 T3966 themeOf TNF-α,production
R3249 T3966 T3964 themeOf production,induced
R5366 T6478 T6477 themeOf I-κBα,degradation
R5874 T7081 T7082 themeOf CREB,binding
R5875 T7082 T7080 themeOf binding,increased
R832 T1127 T1129 themeOf tumor necrosis factor-α,production
R833 T1128 T1127 equivalentTo TNF-α,tumor necrosis factor-α
R834 T1129 T1126 themeOf production,regulator
R835 T1131 T1132 themeOf TNF-α,synthesis
R836 T1132 T1133 themeOf synthesis,attenuated
R837 T1135 T1134 themeOf downregulation,lead
R838 T1136 T1137 themeOf TNF-α,production
R839 T1137 T1135 themeOf production,downregulation
R840 T1139 T1138 themeOf dependent,inhibit
R841 T1140 T1139 themeOf transcription,dependent
R842 T1141 T1140 themeOf TNF-α,transcription
R843 T1143 T1144 themeOf TNF-α,production
R844 T1144 T1142 themeOf production,down-regulates
R845 T1146 T1145 themeOf induced,role
R846 T1147 T1148 themeOf TNF-α,production
R847 T1148 T1146 themeOf production,induced

bionlp-st-ge-2016-coref

Id Subject Object Predicate Lexical cue
T9699 17712-17715 Anaphor denotes its
T9700 17684-17689 Antecedent denotes TNF-α
T9701 23859-23873 Anaphor denotes its components
T9702 23875-23880 Antecedent denotes c-fos
T9703 23885-23890 Antecedent denotes c-jun
R8120 T9699 T9700 boundBy its,TNF-α
R8121 T9701 T9702 boundBy its components,c-fos
R8122 T9701 T9703 boundBy its components,c-jun

2_test

Id Subject Object Predicate Lexical cue
18568240-12168567-22101112 2485-2486 12168567 denotes 2
18568240-9442376-22101113 2614-2615 9442376 denotes 3
18568240-2460096-22101114 2880-2881 2460096 denotes 4
18568240-1847694-22101115 3274-3275 1847694 denotes 5
18568240-12829247-22101116 3493-3494 12829247 denotes 6
18568240-12829247-22101117 3809-3810 12829247 denotes 6
18568240-15044179-22101117 3809-3810 15044179 denotes 6
18568240-15221855-22101117 3809-3810 15221855 denotes 6
18568240-8702838-22101118 4118-4119 8702838 denotes 9
18568240-17689696-22101119 6987-6989 17689696 denotes 10
18568240-17689696-22101120 13112-13114 17689696 denotes 10
18568240-7881165-22101121 17192-17194 7881165 denotes 11
18568240-2785049-22101121 17192-17194 2785049 denotes 11
18568240-3366904-22101121 17192-17194 3366904 denotes 11
18568240-9422758-22101121 17192-17194 9422758 denotes 11
18568240-7861035-22101122 17396-17398 7861035 denotes 15
18568240-7861035-22101123 17559-17561 7861035 denotes 15
18568240-3317066-22101123 17559-17561 3317066 denotes 15
18568240-15625444-22101124 18037-18039 15625444 denotes 18
18568240-14960994-22101124 18037-18039 14960994 denotes 18
18568240-12829247-22101125 18713-18714 12829247 denotes 6
18568240-15044179-22101125 18713-18714 15044179 denotes 6
18568240-15221855-22101125 18713-18714 15221855 denotes 6
18568240-14960994-22101126 19160-19162 14960994 denotes 19
18568240-16456445-22101127 19608-19610 16456445 denotes 20
18568240-11131907-22101127 19608-19610 11131907 denotes 20
18568240-16294063-22101127 19608-19610 16294063 denotes 20
18568240-15105227-22101127 19608-19610 15105227 denotes 20
18568240-16361898-22101127 19608-19610 16361898 denotes 20
18568240-8717528-22101128 20058-20060 8717528 denotes 26
18568240-9346484-22101129 20624-20626 9346484 denotes 27
18568240-9252186-22101130 20627-20629 9252186 denotes 28
18568240-2104921-22101131 20844-20846 2104921 denotes 29
18568240-2249779-22101132 20847-20849 2249779 denotes 30
18568240-11805218-22101133 21405-21407 11805218 denotes 31
18568240-7744006-22101134 21566-21568 7744006 denotes 32
18568240-12230568-22101135 21933-21935 12230568 denotes 33
18568240-8702838-22101136 22149-22150 8702838 denotes 9
18568240-10395470-22101137 23526-23531 10395470 denotes 30034
18568240-12606779-22101138 24087-24089 12606779 denotes 35

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T451 0-8 NN denotes Insights
T452 9-13 IN denotes into
T453 14-17 DT denotes the
T454 18-28 NN denotes Regulation
T455 29-31 IN denotes of
T456 32-37 NN denotes TNF-α
T457 38-48 NN denotes Production
T458 49-51 IN denotes in
T459 52-57 JJ denotes Human
T460 58-69 JJ denotes Mononuclear
T461 70-75 NN denotes Cells
T462 75-76 -COLON- denotes :
T463 77-80 DT denotes The
T464 81-88 NN denotes Effects
T465 89-91 IN denotes of
T466 92-104 JJ denotes Non-Specific
T467 105-122 NN denotes Phosphodiesterase
T468 123-133 NN denotes Inhibition
T469 145-154 NN denotes OBJECTIVE
T470 155-158 DT denotes The
T471 159-168 NN denotes objective
T472 169-171 IN denotes of
T473 172-176 DT denotes this
T474 177-182 NN denotes study
T475 183-186 VB denotes was
T476 187-189 TO denotes to
T477 190-199 VB denotes determine
T478 200-203 DT denotes the
T479 204-210 NN denotes effect
T480 211-213 IN denotes of
T481 214-225 JJ denotes nonspecific
T482 226-243 NN denotes phosphodiesterase
T483 244-254 NN denotes inhibition
T484 255-257 IN denotes on
T485 258-271 NN denotes transcription
T486 272-278 NN denotes factor
T487 279-289 NN denotes activation
T488 290-293 CC denotes and
T489 294-299 NN denotes tumor
T490 300-308 NN denotes necrosis
T491 309-321 NN denotes factor-alpha
T492 322-323 -LRB- denotes (
T493 323-328 NN denotes TNF-α
T494 328-329 -RRB- denotes )
T495 330-340 NN denotes production
T496 341-343 IN denotes in
T497 344-362 NN denotes lipopolysaccharide
T498 363-364 -LRB- denotes (
T499 364-367 NN denotes LPS
T500 367-368 -RRB- denotes )
T501 368-379 JJ denotes -stimulated
T502 380-385 JJ denotes human
T503 386-397 JJ denotes mononuclear
T504 398-403 NN denotes cells
T505 406-418 NN denotes INTRODUCTION
T506 419-422 DT denotes The
T507 423-433 NN denotes production
T508 434-436 IN denotes of
T509 437-442 NN denotes TNF-α
T510 443-452 VB denotes following
T511 453-456 NN denotes LPS
T512 457-468 NN denotes stimulation
T513 469-471 VB denotes is
T514 472-475 CD denotes one
T515 476-478 IN denotes of
T516 479-482 DT denotes the
T517 483-486 JJ denotes key
T518 487-492 NN denotes steps
T519 493-495 IN denotes in
T520 496-505 JJ denotes bacterial
T521 506-512 NN denotes sepsis
T522 513-516 CC denotes and
T523 517-529 NN denotes inflammation
T524 531-534 DT denotes The
T525 535-544 NN denotes mechanism
T526 545-547 IN denotes by
T527 548-553 WDT denotes which
T528 554-571 NN denotes phosphodiesterase
T529 572-582 NN denotes inhibition
T530 583-589 VB denotes alters
T531 590-595 NN denotes TNF-α
T532 596-606 NN denotes production
T533 607-609 IN denotes in
T534 610-613 DT denotes the
T535 614-622 NN denotes presence
T536 623-625 IN denotes of
T537 626-629 NN denotes LPS
T538 630-637 VB denotes remains
T539 638-645 JJ denotes unclear
T540 648-655 NN denotes METHODS
T541 656-661 JJ denotes Human
T542 662-673 JJ denotes mononuclear
T543 674-679 NN denotes cells
T544 680-684 VB denotes were
T545 685-695 VB denotes stimulated
T546 696-700 IN denotes with
T547 701-704 NN denotes LPS
T548 705-706 -LRB- denotes (
T549 706-707 CD denotes 1
T550 708-713 NN denotes μg/mL
T551 713-714 -RRB- denotes )
T552 714-715 -COMMA- denotes ,
T553 716-718 IN denotes in
T554 719-722 DT denotes the
T555 723-731 NN denotes presence
T556 732-735 CC denotes and
T557 736-743 NN denotes absence
T558 744-746 IN denotes of
T559 747-761 NN denotes Pentoxifylline
T560 762-763 -LRB- denotes (
T561 763-766 NN denotes PTX
T562 766-767 -COLON- denotes ;
T563 768-770 CD denotes 20
T564 771-773 NN denotes mM
T565 773-774 -RRB- denotes )
T566 774-775 -COMMA- denotes ,
T567 776-777 DT denotes a
T568 778-789 JJ denotes nonspecific
T569 790-807 NN denotes phosphodiesterase
T570 808-817 NN denotes inhibitor
T571 819-826 JJ denotes Western
T572 827-835 NN denotes blotting
T573 836-838 IN denotes of
T574 839-853 VB denotes phosphorylated
T575 854-865 JJ denotes cytoplasmic
T576 866-871 NN denotes I-κBα
T577 871-872 -COMMA- denotes ,
T578 873-880 JJ denotes nuclear
T579 881-890 NN denotes factor-κB
T580 891-894 NN denotes p65
T581 895-896 -LRB- denotes (
T582 896-901 NN denotes NF-κB
T583 901-902 -RRB- denotes )
T584 902-903 -COMMA- denotes ,
T585 904-907 CC denotes and
T586 908-915 JJ denotes nuclear
T587 916-929 JJ denotes cAMP-response
T588 930-937 NN denotes element
T589 938-945 NN denotes binding
T590 946-953 NN denotes protein
T591 954-955 -LRB- denotes (
T592 955-959 NN denotes CREB
T593 959-960 -RRB- denotes )
T594 961-964 VB denotes was
T595 965-974 VB denotes performed
T596 976-979 NN denotes DNA
T597 980-987 NN denotes binding
T598 988-990 IN denotes of
T599 991-996 NN denotes NF-κB
T600 997-1000 CC denotes and
T601 1001-1005 NN denotes CREB
T602 1006-1009 VB denotes was
T603 1010-1018 VB denotes verified
T604 1019-1021 IN denotes by
T605 1022-1037 JJ denotes electrophoretic
T606 1038-1046 NN denotes mobility
T607 1047-1052 NN denotes shift
T608 1053-1058 NN denotes assay
T609 1060-1065 NN denotes TNF-α
T610 1066-1072 NN denotes levels
T611 1073-1077 VB denotes were
T612 1078-1088 VB denotes determined
T613 1089-1091 IN denotes in
T614 1092-1095 DT denotes the
T615 1096-1107 NN denotes supernatant
T616 1108-1110 IN denotes of
T617 1111-1121 VB denotes stimulated
T618 1122-1127 NN denotes cells
T619 1128-1130 IN denotes in
T620 1131-1134 DT denotes the
T621 1135-1143 NN denotes presence
T622 1144-1147 CC denotes and
T623 1148-1155 NN denotes absence
T624 1156-1163 NN denotes Protein
T625 1164-1170 NN denotes kinase
T626 1171-1172 NN denotes A
T627 1173-1183 NN denotes inhibition
T628 1184-1186 IN denotes by
T629 1187-1189 DT denotes an
T630 1190-1203 JJ denotes enzyme-linked
T631 1204-1217 JJ denotes immunosorbent
T632 1218-1223 NN denotes assay
T633 1224-1225 -LRB- denotes (
T634 1225-1230 NN denotes ELISA
T635 1230-1231 -RRB- denotes )
T636 1234-1241 NN denotes RESULTS
T637 1242-1245 NN denotes PTX
T638 1246-1249 VB denotes was
T639 1250-1262 VB denotes demonstrated
T640 1263-1265 TO denotes to
T641 1266-1279 RB denotes significantly
T642 1280-1286 VB denotes reduce
T643 1287-1298 JJ denotes cytoplasmic
T644 1299-1304 NN denotes I-κBα
T645 1305-1320 NN denotes phosphorylation
T646 1320-1321 -COMMA- denotes ,
T647 1322-1329 JJ denotes nuclear
T648 1330-1333 NN denotes p65
T649 1334-1349 NN denotes phosphorylation
T650 1349-1350 -COMMA- denotes ,
T651 1351-1354 CC denotes and
T652 1355-1358 DT denotes the
T653 1359-1362 NN denotes DNA
T654 1363-1370 NN denotes binding
T655 1371-1379 NN denotes activity
T656 1380-1382 IN denotes of
T657 1383-1388 NN denotes NF-κB
T658 1390-1392 IN denotes In
T659 1393-1401 NN denotes contrast
T660 1401-1402 -COMMA- denotes ,
T661 1403-1406 NN denotes PTX
T662 1407-1415 RB denotes markedly
T663 1416-1424 VB denotes enhanced
T664 1425-1428 DT denotes the
T665 1429-1444 NN denotes phosphorylation
T666 1445-1448 CC denotes and
T667 1449-1452 NN denotes DNA
T668 1453-1460 NN denotes binding
T669 1461-1469 NN denotes activity
T670 1470-1472 IN denotes of
T671 1473-1477 NN denotes CREB
T672 1479-1484 NN denotes Cells
T673 1485-1498 RB denotes concomitantly
T674 1499-1506 VB denotes treated
T675 1507-1511 IN denotes with
T676 1512-1515 NN denotes PTX
T677 1516-1519 CC denotes and
T678 1520-1523 NN denotes LPS
T679 1524-1532 VB denotes secreted
T680 1533-1540 JJ denotes similar
T681 1541-1547 NN denotes levels
T682 1548-1550 IN denotes of
T683 1551-1556 NN denotes TNF-α
T684 1557-1559 IN denotes in
T685 1560-1563 DT denotes the
T686 1564-1572 NN denotes presence
T687 1573-1576 CC denotes and
T688 1577-1584 NN denotes absence
T689 1585-1592 NN denotes Protein
T690 1593-1599 NN denotes kinase
T691 1600-1601 NN denotes A
T692 1602-1612 NN denotes inhibition
T693 1615-1625 NN denotes DISCUSSION
T694 1626-1629 DT denotes The
T695 1630-1639 VB denotes increased
T696 1640-1645 NN denotes level
T697 1646-1648 IN denotes of
T698 1649-1653 NN denotes cAMP
T699 1654-1658 WDT denotes that
T700 1659-1666 VB denotes results
T701 1667-1671 IN denotes from
T702 1672-1689 NN denotes phosphodiesterase
T703 1690-1700 NN denotes inhibition
T704 1701-1708 VB denotes affects
T705 1709-1720 JJ denotes cytoplasmic
T706 1721-1724 CC denotes and
T707 1725-1732 JJ denotes nuclear
T708 1733-1739 NN denotes events
T709 1739-1740 -COMMA- denotes ,
T710 1741-1750 VB denotes resulting
T711 1751-1753 IN denotes in
T712 1754-1757 DT denotes the
T713 1758-1769 NN denotes attenuation
T714 1770-1772 IN denotes of
T715 1773-1778 NN denotes NF-κB
T716 1779-1782 CC denotes and
T717 1783-1786 DT denotes the
T718 1787-1797 NN denotes activation
T719 1798-1800 IN denotes of
T720 1801-1805 NN denotes CREB
T721 1806-1821 JJ denotes transcriptional
T722 1822-1825 NN denotes DNA
T723 1826-1833 NN denotes binding
T724 1834-1841 IN denotes through
T725 1842-1850 NN denotes pathways
T726 1851-1855 WDT denotes that
T727 1856-1859 VB denotes are
T728 1860-1869 RB denotes partially
T729 1870-1877 NN denotes Protein
T730 1878-1884 NN denotes kinase
T731 1885-1898 JJ denotes A-independent
T732 1901-1911 NN denotes CONCLUSION
T733 1912-1924 JJ denotes PTX-mediated
T734 1925-1942 NN denotes phosphodiesterase
T735 1943-1953 NN denotes inhibition
T736 1954-1960 VB denotes occurs
T737 1961-1970 RB denotes partially
T738 1971-1978 IN denotes through
T739 1979-1980 DT denotes a
T740 1981-1988 NN denotes Protein
T741 1989-1995 NN denotes kinase
T742 1996-2009 JJ denotes A-independent
T743 2010-2017 NN denotes pathway
T744 2018-2021 CC denotes and
T745 2022-2025 MD denotes may
T746 2026-2031 VB denotes serve
T747 2032-2034 IN denotes as
T748 2035-2036 DT denotes a
T749 2037-2043 JJ denotes useful
T750 2044-2048 NN denotes tool
T751 2049-2051 IN denotes in
T752 2052-2055 DT denotes the
T753 2056-2067 NN denotes attenuation
T754 2068-2070 IN denotes of
T755 2071-2082 JJ denotes LPS-induced
T756 2083-2095 NN denotes inflammation
T1612 2112-2115 DT denotes The
T1613 2116-2125 JJ denotes bacterial
T1614 2126-2134 NN denotes membrane
T1615 2135-2144 NN denotes component
T1616 2145-2163 NN denotes lipopolysaccharide
T1617 2164-2165 -LRB- denotes (
T1618 2165-2168 NN denotes LPS
T1619 2168-2169 -RRB- denotes )
T1620 2170-2172 VB denotes is
T1621 2173-2180 JJ denotes capable
T1622 2181-2183 IN denotes of
T1623 2184-2194 VB denotes initiating
T1624 2195-2210 NN denotes phosphorylation
T1625 2211-2214 CC denotes and
T1626 2215-2225 NN denotes activation
T1627 2226-2228 IN denotes of
T1628 2229-2237 JJ denotes multiple
T1629 2238-2242 NN denotes host
T1630 2243-2256 JJ denotes intracellular
T1631 2257-2264 NN denotes protein
T1632 2265-2272 NN denotes kinases
T1633 2273-2276 CC denotes and
T1634 2277-2290 NN denotes transcription
T1635 2291-2298 NN denotes factors
T1636 2300-2313 NN denotes Transcription
T1637 2314-2320 NN denotes factor
T1638 2321-2331 NN denotes activation
T1639 2331-2332 -COMMA- denotes ,
T1640 2333-2338 WDT denotes which
T1641 2339-2346 VB denotes results
T1642 2347-2349 IN denotes in
T1643 2350-2353 DT denotes the
T1644 2354-2364 NN denotes modulation
T1645 2365-2367 IN denotes of
T1646 2368-2372 NN denotes gene
T1647 2373-2386 NN denotes transcription
T1648 2387-2390 CC denotes and
T1649 2391-2398 NN denotes protein
T1650 2399-2408 NN denotes synthesis
T1651 2408-2409 -COMMA- denotes ,
T1652 2410-2412 VB denotes is
T1653 2413-2414 DT denotes a
T1654 2415-2423 JJ denotes critical
T1655 2424-2431 NN denotes element
T1656 2432-2434 IN denotes in
T1657 2435-2438 DT denotes the
T1658 2439-2446 NN denotes defense
T1659 2447-2456 NN denotes mechanism
T1660 2457-2459 IN denotes of
T1661 2460-2463 DT denotes the
T1662 2464-2468 NN denotes host
T1663 2469-2475 JJ denotes immune
T1664 2476-2486 NN denotes system.1,2
T1665 2487-2498 JJ denotes LPS-induced
T1666 2499-2512 NN denotes transcription
T1667 2513-2519 NN denotes factor
T1668 2520-2530 NN denotes activation
T1669 2531-2534 VB denotes has
T1670 2535-2539 VB denotes been
T1671 2540-2545 VB denotes shown
T1672 2546-2548 TO denotes to
T1673 2549-2551 VB denotes be
T1674 2552-2553 DT denotes a
T1675 2554-2557 JJ denotes key
T1676 2558-2567 NN denotes regulator
T1677 2568-2570 IN denotes of
T1678 2571-2576 NN denotes tumor
T1679 2577-2585 NN denotes necrosis
T1680 2586-2594 NN denotes factor-α
T1681 2595-2596 -LRB- denotes (
T1682 2596-2601 NN denotes TNF-α
T1683 2601-2602 -RRB- denotes )
T1684 2603-2615 NN denotes production.3
T1685 2616-2621 NN denotes TNF-α
T1686 2622-2624 VB denotes is
T1687 2625-2626 DT denotes a
T1688 2627-2633 JJ denotes potent
T1689 2634-2650 JJ denotes pro-inflammatory
T1690 2651-2659 NN denotes cytokine
T1691 2660-2668 VB denotes involved
T1692 2669-2671 IN denotes in
T1693 2672-2673 DT denotes a
T1694 2674-2678 JJ denotes wide
T1695 2679-2687 NN denotes spectrum
T1696 2688-2690 IN denotes of
T1697 2691-2699 JJ denotes cellular
T1698 2700-2709 NN denotes responses
T1699 2711-2722 RB denotes Furthermore
T1700 2722-2723 -COMMA- denotes ,
T1701 2724-2729 NN denotes TNF-α
T1702 2730-2739 NN denotes synthesis
T1703 2740-2743 MD denotes can
T1704 2744-2746 VB denotes be
T1705 2747-2757 VB denotes attenuated
T1706 2758-2760 IN denotes in
T1707 2761-2767 JJ denotes immune
T1708 2768-2773 NN denotes cells
T1709 2774-2781 VB denotes exposed
T1710 2782-2784 TO denotes to
T1711 2785-2802 NN denotes phosphodiesterase
T1712 2803-2804 -LRB- denotes (
T1713 2804-2807 NN denotes PDE
T1714 2807-2808 -RRB- denotes )
T1715 2809-2819 NN denotes inhibition
T1716 2820-2825 IN denotes after
T1717 2826-2835 NN denotes challenge
T1718 2836-2838 IN denotes by
T1719 2839-2840 DT denotes a
T1720 2841-2848 NN denotes variety
T1721 2849-2851 IN denotes of
T1722 2852-2868 JJ denotes pro-inflammatory
T1723 2869-2881 NN denotes stimulants.4
T1724 2882-2885 DT denotes The
T1725 2886-2895 NN denotes signaling
T1726 2896-2906 NN denotes mechanisms
T1727 2907-2915 VB denotes affected
T1728 2916-2918 IN denotes by
T1729 2919-2922 NN denotes PDE
T1730 2923-2933 NN denotes inhibition
T1731 2933-2934 -COMMA- denotes ,
T1732 2935-2940 WDT denotes which
T1733 2941-2951 RB denotes ultimately
T1734 2952-2956 VB denotes lead
T1735 2957-2959 TO denotes to
T1736 2960-2963 DT denotes the
T1737 2964-2978 NN denotes downregulation
T1738 2979-2981 IN denotes of
T1739 2982-2987 NN denotes TNF-α
T1740 2988-2998 NN denotes production
T1741 2998-2999 -COMMA- denotes ,
T1742 3000-3004 VB denotes have
T1743 3005-3008 RB denotes not
T1744 3009-3013 VB denotes been
T1745 3014-3018 RB denotes well
T1746 3019-3032 VB denotes characterized
T1747 3033-3035 IN denotes in
T1748 3036-3048 JJ denotes inflammatory
T1749 3049-3054 NN denotes cells
T1750 3056-3067 RB denotes Classically
T1751 3067-3068 -COMMA- denotes ,
T1752 3069-3071 PRP denotes it
T1753 3072-3075 VB denotes has
T1754 3076-3080 VB denotes been
T1755 3081-3093 VB denotes demonstrated
T1756 3094-3098 IN denotes that
T1757 3099-3102 NN denotes PDE
T1758 3103-3113 NN denotes inhibition
T1759 3114-3121 VB denotes results
T1760 3122-3124 IN denotes in
T1761 3125-3128 DT denotes the
T1762 3129-3142 JJ denotes intracellular
T1763 3143-3155 NN denotes accumulation
T1764 3156-3158 IN denotes of
T1765 3159-3162 DT denotes the
T1766 3163-3169 JJ denotes second
T1767 3170-3179 NN denotes messenger
T1768 3180-3186 JJ denotes cyclic
T1769 3187-3214 NN denotes adenosine-3,5-monophosphate
T1770 3215-3216 -LRB- denotes (
T1771 3216-3220 NN denotes cAMP
T1772 3220-3221 -RRB- denotes )
T1773 3222-3225 CC denotes and
T1774 3226-3236 JJ denotes subsequent
T1775 3237-3247 NN denotes activation
T1776 3248-3250 IN denotes of
T1777 3251-3258 NN denotes Protein
T1778 3259-3265 NN denotes kinase
T1779 3266-3267 NN denotes A
T1780 3268-3269 -LRB- denotes (
T1781 3269-3272 NN denotes PKA
T1782 3272-3273 -RRB- denotes )
T1783 3273-3275 CD denotes .5
T1784 3276-3279 NN denotes PKA
T1785 3280-3290 NN denotes activation
T1786 3291-3295 RB denotes then
T1787 3296-3301 VB denotes leads
T1788 3302-3304 TO denotes to
T1789 3305-3308 DT denotes the
T1790 3309-3324 NN denotes phosphorylation
T1791 3325-3327 IN denotes of
T1792 3328-3331 DT denotes the
T1793 3332-3345 NN denotes transcription
T1794 3346-3352 NN denotes factor
T1795 3353-3366 JJ denotes cAMP-response
T1796 3367-3374 NN denotes element
T1797 3375-3382 NN denotes binding
T1798 3383-3390 NN denotes protein
T1799 3391-3392 -LRB- denotes (
T1800 3392-3396 NN denotes CREB
T1801 3396-3397 -RRB- denotes )
T1802 3397-3398 -COMMA- denotes ,
T1803 3399-3411 NN denotes transmission
T1804 3412-3414 IN denotes of
T1805 3415-3422 NN denotes signals
T1806 3423-3427 IN denotes into
T1807 3428-3431 DT denotes the
T1808 3432-3439 NN denotes nucleus
T1809 3439-3440 -COMMA- denotes ,
T1810 3441-3444 CC denotes and
T1811 3445-3448 DT denotes the
T1812 3449-3459 JJ denotes subsequent
T1813 3460-3470 NN denotes modulation
T1814 3471-3473 IN denotes of
T1815 3474-3478 NN denotes gene
T1816 3479-3494 NN denotes transcription.6
T1817 3495-3499 DT denotes This
T1818 3500-3510 RB denotes apparently
T1819 3511-3517 JJ denotes simple
T1820 3518-3524 JJ denotes linear
T1821 3525-3532 NN denotes cascade
T1822 3533-3537 VB denotes does
T1823 3538-3541 RB denotes not
T1824 3542-3547 RB denotes fully
T1825 3548-3555 VB denotes explain
T1826 3556-3559 DT denotes the
T1827 3560-3569 NN denotes mechanism
T1828 3570-3572 IN denotes by
T1829 3573-3578 WDT denotes which
T1830 3579-3581 DT denotes an
T1831 3582-3591 NN denotes elevation
T1832 3592-3594 IN denotes in
T1833 3595-3598 DT denotes the
T1834 3599-3612 JJ denotes intracellular
T1835 3613-3617 NN denotes cAMP
T1836 3618-3623 NN denotes level
T1837 3624-3630 VB denotes exerts
T1838 3631-3643 JJ denotes wide-ranging
T1839 3644-3651 NN denotes effects
T1840 3652-3654 IN denotes on
T1841 3655-3663 JJ denotes multiple
T1842 3664-3672 JJ denotes cellular
T1843 3673-3682 NN denotes functions
T1844 3684-3689 EX denotes There
T1845 3690-3692 VB denotes is
T1846 3693-3694 DT denotes a
T1847 3695-3702 VB denotes growing
T1848 3703-3707 NN denotes body
T1849 3708-3710 IN denotes of
T1850 3711-3719 NN denotes evidence
T1851 3720-3730 VB denotes suggesting
T1852 3731-3735 IN denotes that
T1853 3736-3740 NN denotes cAMP
T1854 3741-3744 MD denotes may
T1855 3745-3753 VB denotes function
T1856 3754-3761 IN denotes through
T1857 3762-3766 CC denotes both
T1858 3767-3780 JJ denotes PKA-dependent
T1859 3781-3784 CC denotes and
T1860 3785-3797 JJ denotes -independent
T1861 3798-3812 NN denotes mechanisms.6–8
T1862 3813-3824 JJ denotes LPS-induced
T1863 3825-3835 NN denotes activation
T1864 3836-3838 IN denotes of
T1865 3839-3842 DT denotes the
T1866 3843-3856 NN denotes transcription
T1867 3857-3863 NN denotes factor
T1868 3864-3871 JJ denotes nuclear
T1869 3872-3881 NN denotes factor-κB
T1870 3882-3883 -LRB- denotes (
T1871 3883-3888 NN denotes NF-κB
T1872 3888-3889 -RRB- denotes )
T1873 3890-3893 VB denotes has
T1874 3894-3898 RB denotes also
T1875 3899-3903 VB denotes been
T1876 3904-3907 DT denotes the
T1877 3908-3913 NN denotes focus
T1878 3914-3916 IN denotes of
T1879 3917-3918 DT denotes a
T1880 3919-3924 JJ denotes great
T1881 3925-3929 NN denotes deal
T1882 3930-3932 IN denotes of
T1883 3933-3941 NN denotes research
T1884 3943-3945 PRP denotes It
T1885 3946-3949 VB denotes has
T1886 3950-3954 VB denotes been
T1887 3955-3962 RB denotes clearly
T1888 3963-3975 VB denotes demonstrated
T1889 3976-3980 IN denotes that
T1890 3981-3987 NN denotes agents
T1891 3988-3992 WDT denotes that
T1892 3993-4001 VB denotes increase
T1893 4002-4015 JJ denotes intracellular
T1894 4016-4020 NN denotes cAMP
T1895 4021-4025 RB denotes also
T1896 4026-4033 VB denotes inhibit
T1897 4034-4049 JJ denotes NF-κB-dependent
T1898 4050-4066 JJ denotes pro-inflammatory
T1899 4067-4071 NN denotes gene
T1900 4072-4085 NN denotes transcription
T1901 4085-4086 -COMMA- denotes ,
T1902 4087-4099 RB denotes particularly
T1903 4100-4102 IN denotes of
T1904 4103-4106 DT denotes the
T1905 4107-4112 NN denotes TNF-α
T1906 4113-4119 NN denotes gene.9
T1907 4120-4131 NNP denotes Controversy
T1908 4132-4138 VB denotes exists
T1909 4139-4148 VB denotes regarding
T1910 4149-4152 DT denotes the
T1911 4153-4158 JJ denotes exact
T1912 4159-4168 NN denotes mechanism
T1913 4168-4169 -LRB- denotes (
T1914 4169-4170 NN denotes s
T1915 4170-4171 -RRB- denotes )
T1916 4172-4174 IN denotes by
T1917 4175-4180 WDT denotes which
T1918 4181-4184 NN denotes PDE
T1919 4185-4195 NN denotes inhibition
T1920 4196-4210 VB denotes down-regulates
T1921 4211-4216 NN denotes TNF-α
T1922 4217-4227 NN denotes production
T1923 4229-4242 NN denotes Possibilities
T1924 4243-4250 VB denotes include
T1925 4250-4251 -COMMA- denotes ,
T1926 4252-4255 CC denotes but
T1927 4256-4259 VB denotes are
T1928 4260-4263 RB denotes not
T1929 4264-4271 VB denotes limited
T1930 4272-4274 TO denotes to
T1931 4274-4275 -COMMA- denotes ,
T1932 4276-4286 NN denotes inhibition
T1933 4287-4289 IN denotes of
T1934 4290-4295 NN denotes NF-κB
T1935 4296-4299 NN denotes DNA
T1936 4300-4307 NN denotes binding
T1937 4308-4316 NN denotes activity
T1938 4316-4317 -COMMA- denotes ,
T1939 4318-4332 NN denotes downregulation
T1940 4333-4335 IN denotes of
T1941 4336-4341 NN denotes NF-κB
T1942 4342-4357 JJ denotes transcriptional
T1943 4358-4366 NN denotes activity
T1944 4366-4367 -COMMA- denotes ,
T1945 4368-4377 VB denotes increased
T1946 4378-4382 NN denotes CREB
T1947 4383-4393 NN denotes activation
T1948 4393-4394 -COMMA- denotes ,
T1949 4395-4398 CC denotes and
T1950 4399-4410 NN denotes competition
T1951 4411-4418 IN denotes between
T1952 4419-4424 NN denotes NF-κB
T1953 4425-4428 CC denotes and
T1954 4429-4433 NN denotes CREB
T1955 4434-4437 IN denotes for
T1956 4438-4444 JJ denotes common
T1957 4445-4458 NN denotes co-activators
T1958 4459-4463 JJ denotes such
T1959 4464-4466 IN denotes as
T1960 4467-4471 NN denotes CREB
T1961 4472-4479 NN denotes binding
T1962 4480-4487 NN denotes protein
T1963 4488-4489 -LRB- denotes (
T1964 4489-4492 NN denotes CBP
T1965 4492-4493 -RRB- denotes )
T1966 4495-4497 PRP denotes It
T1967 4498-4500 VB denotes is
T1968 4501-4505 RB denotes also
T1969 4506-4509 RB denotes not
T1970 4510-4515 VB denotes known
T1971 4516-4523 IN denotes whether
T1972 4524-4529 DT denotes these
T1973 4530-4539 NN denotes processes
T1974 4540-4544 VB denotes rely
T1975 4545-4551 RB denotes solely
T1976 4552-4556 IN denotes upon
T1977 4557-4560 DT denotes the
T1978 4561-4571 NN denotes activation
T1979 4572-4574 IN denotes of
T1980 4575-4578 NN denotes PKA
T1981 4580-4589 RB denotes Therefore
T1982 4589-4590 -COMMA- denotes ,
T1983 4591-4594 DT denotes the
T1984 4595-4604 NN denotes objective
T1985 4605-4607 IN denotes of
T1986 4608-4611 DT denotes the
T1987 4612-4619 JJ denotes present
T1988 4620-4625 NN denotes study
T1989 4626-4628 VB denotes is
T1990 4629-4631 TO denotes to
T1991 4632-4641 VB denotes determine
T1992 4642-4645 DT denotes the
T1993 4646-4653 NN denotes effects
T1994 4654-4656 IN denotes of
T1995 4657-4668 JJ denotes nonspecific
T1996 4669-4672 NN denotes PDE
T1997 4673-4683 NN denotes inhibition
T1998 4684-4688 IN denotes with
T1999 4689-4691 NN denotes 1-
T2000 4691-4692 -LRB- denotes [
T2001 4692-4702 NN denotes 5-oxohexyl
T2002 4702-4703 -RRB- denotes ]
T2003 4703-4724 NN denotes -3,7-dimethylxanthine
T2004 4725-4726 -LRB- denotes (
T2005 4726-4740 NN denotes Pentoxifylline
T2006 4740-4741 -COLON- denotes ;
T2007 4742-4745 NN denotes PTX
T2008 4745-4746 -RRB- denotes )
T2009 4747-4749 IN denotes on
T2010 4750-4755 NN denotes NF-κB
T2011 4756-4759 CC denotes and
T2012 4760-4764 NN denotes CREB
T2013 4765-4775 NN denotes activation
T2014 4776-4778 FW denotes in
T2015 4779-4784 FW denotes vitro
T2016 4785-4787 IN denotes in
T2017 4788-4793 JJ denotes human
T2018 4794-4805 JJ denotes mononuclear
T2019 4806-4811 NN denotes cells
T2020 4813-4817 IN denotes With
T2021 4818-4821 DT denotes the
T2022 4822-4825 NN denotes use
T2023 4826-4828 IN denotes of
T2024 4829-4837 JJ denotes specific
T2025 4838-4848 NN denotes inhibitors
T2026 4848-4849 -COMMA- denotes ,
T2027 4850-4852 PRP denotes we
T2028 4853-4857 RB denotes also
T2029 4858-4870 VB denotes investigated
T2030 4871-4874 DT denotes the
T2031 4875-4879 NN denotes role
T2032 4880-4882 IN denotes of
T2033 4883-4886 NN denotes PKA
T2034 4887-4889 IN denotes in
T2035 4890-4901 JJ denotes LPS-induced
T2036 4902-4907 NN denotes TNF-α
T2037 4908-4918 NN denotes production
T2791 172-176 DT denotes this
T2792 177-182 NN denotes study
T2793 183-186 VB denotes was
T2794 4958-4966 VB denotes approved
T2795 4967-4969 IN denotes by
T2796 4970-4973 DT denotes the
T2797 4974-4979 NNP denotes Human
T2798 4980-4988 NNP denotes Research
T2799 4989-5000 NNP denotes Protections
T2800 5001-5008 NNP denotes Program
T2801 5009-5012 CC denotes and
T2802 5013-5016 DT denotes the
T2803 5017-5030 NNP denotes Institutional
T2804 5031-5037 NNP denotes Review
T2805 5038-5044 NNP denotes Board.
T2806 5045-5052 NNP denotes Written
T2807 5053-5060 NN denotes consent
T2808 5061-5063 TO denotes to
T2809 5064-5075 VB denotes participate
T2810 5076-5078 IN denotes in
T2811 5079-5082 DT denotes the
T2812 5083-5088 NN denotes study
T2813 5089-5092 VB denotes was
T2814 5093-5101 VB denotes obtained
T2815 5102-5106 IN denotes from
T2816 5107-5110 DT denotes all
T2817 5111-5121 NN denotes volunteers
T2818 5122-5127 RB denotes prior
T2819 5128-5130 TO denotes to
T2820 5131-5136 NN denotes blood
T2821 5137-5158 JJ denotes donation. Heparinized
T2822 5159-5170 NN denotes Vacutainers
T2823 5171-5175 VB denotes were
T2824 5176-5185 VB denotes purchased
T2825 5186-5190 IN denotes from
T2826 5191-5197 NNP denotes Becton
T2827 5198-5207 NNP denotes Dickinson
T2828 5208-5209 -LRB- denotes (
T2829 5209-5212 NNP denotes San
T2830 5213-5217 NNP denotes Jose
T2831 5217-5218 -COMMA- denotes ,
T2832 5219-5221 NNP denotes CA
T2833 5221-5222 -RRB- denotes )
T2834 5224-5230 NN denotes 1.5-mL
T2835 5231-5240 NNP denotes Eppendorf
T2836 5241-5251 NN denotes centrifuge
T2837 5252-5257 NN denotes tubes
T2838 5258-5262 VB denotes were
T2839 5263-5272 VB denotes purchased
T2840 5273-5277 IN denotes from
T2841 5278-5284 NNP denotes Fisher
T2842 5285-5295 NNP denotes Scientific
T2843 5296-5297 -LRB- denotes (
T2844 5297-5306 NNP denotes Pittsburg
T2845 5306-5307 -COMMA- denotes ,
T2846 5308-5310 NNP denotes PA
T2847 5310-5311 -RRB- denotes )
T2848 5313-5319 NN denotes Hank’s
T2849 5320-5328 JJ denotes balanced
T2850 5329-5333 NN denotes salt
T2851 5334-5342 NN denotes solution
T2852 5343-5344 -LRB- denotes (
T2853 5344-5348 NN denotes HBSS
T2854 5348-5349 -RRB- denotes )
T2855 5350-5353 CC denotes and
T2856 5354-5358 NN denotes RPMI
T2857 5359-5363 CD denotes 1640
T2858 5364-5368 VB denotes were
T2859 5369-5377 VB denotes obtained
T2860 5378-5382 IN denotes from
T2861 5383-5389 NNP denotes Irvine
T2862 5390-5400 NNP denotes Scientific
T2863 5401-5402 -LRB- denotes (
T2864 5402-5407 NNP denotes Santa
T2865 5408-5411 NNP denotes Ana
T2866 5411-5412 -COMMA- denotes ,
T2867 5413-5415 NNP denotes CA
T2868 5415-5416 -RRB- denotes )
T2869 5418-5421 NN denotes LPS
T2870 5422-5438 NN denotes from Escherichia
T2871 5439-5452 NN denotes coli serotype
T2872 5453-5457 CD denotes 0111
T2873 5457-5458 -COLON- denotes :
T2874 5458-5460 NN denotes B4
T2875 5461-5464 CC denotes and
T2876 5465-5468 NN denotes PTX
T2877 5469-5473 VB denotes were
T2878 5474-5483 VB denotes purchased
T2879 5484-5488 IN denotes from
T2880 5489-5494 NN denotes Sigma
T2881 5495-5496 -LRB- denotes (
T2882 5496-5499 NNP denotes St.
T2883 5500-5505 NNP denotes Louis
T2884 5505-5506 -COMMA- denotes ,
T2885 5507-5509 NN denotes MO
T2886 5509-5510 -RRB- denotes )
T2887 5512-5515 DT denotes The
T2888 5516-5519 NN denotes PKA
T2889 5520-5529 NN denotes inhibitor
T2890 5529-5530 -COMMA- denotes ,
T2891 5531-5533 NN denotes N-
T2892 5533-5534 -LRB- denotes [
T2893 5534-5536 CD denotes 2-
T2894 5536-5537 -LRB- denotes (
T2895 5537-5538 -LRB- denotes (
T2896 5538-5553 NN denotes p-bromocinnamyl
T2897 5553-5554 -RRB- denotes )
T2898 5554-5559 NN denotes amino
T2899 5559-5560 -RRB- denotes )
T2900 5560-5565 NN denotes ethyl
T2901 5565-5566 -RRB- denotes ]
T2902 5566-5597 NN denotes -5-isoguinolinesulfonamide-2HCl
T2903 5597-5598 -RRB- denotes ]
T2904 5599-5600 -LRB- denotes (
T2905 5600-5603 NN denotes H89
T2906 5603-5604 -RRB- denotes )
T2907 5604-5605 -COMMA- denotes ,
T2908 5606-5609 VB denotes was
T2909 5610-5619 VB denotes purchased
T2910 5620-5624 IN denotes from
T2911 5625-5635 NN denotes Calbiochem
T2912 5636-5637 -LRB- denotes (
T2913 5637-5639 NNP denotes La
T2914 5640-5645 NNP denotes Jolla
T2915 5645-5646 -COMMA- denotes ,
T2916 5647-5649 NNP denotes CA
T2917 5649-5650 -RRB- denotes )
T2918 5652-5662 NN denotes Antibodies
T2919 5663-5666 IN denotes for
T2920 5667-5681 VB denotes phosphorylated
T2921 5682-5686 NN denotes CREB
T2922 5687-5688 -LRB- denotes (
T2923 5688-5694 NN denotes serine
T2924 5695-5698 CD denotes 133
T2925 5698-5699 -RRB- denotes )
T2926 5699-5700 -COMMA- denotes ,
T2927 5701-5715 VB denotes phosphorylated
T2928 5716-5721 NN denotes I-κBα
T2929 5722-5723 -LRB- denotes (
T2930 5723-5730 NN denotes serines
T2931 5731-5733 CD denotes 32
T2932 5734-5737 CC denotes and
T2933 5738-5740 CD denotes 36
T2934 5740-5741 -RRB- denotes )
T2935 5741-5742 -COMMA- denotes ,
T2936 5743-5757 VB denotes phosphorylated
T2937 5758-5763 NN denotes NF-κB
T2938 5764-5767 NN denotes p65
T2939 5768-5775 NN denotes subunit
T2940 5776-5777 -LRB- denotes (
T2941 5777-5783 NN denotes serine
T2942 5784-5787 CD denotes 276
T2943 5787-5788 -RRB- denotes )
T2944 5788-5789 -COMMA- denotes ,
T2945 5790-5793 CC denotes and
T2946 5794-5803 JJ denotes secondary
T2947 5804-5814 NN denotes antibodies
T2948 5815-5819 VB denotes were
T2949 5820-5829 VB denotes purchased
T2950 5830-5834 IN denotes from
T2951 5835-5839 NN denotes Cell
T2952 5840-5849 NN denotes Signaling
T2953 5850-5851 -LRB- denotes (
T2954 5851-5858 NNP denotes Beverly
T2955 5858-5859 -COMMA- denotes ,
T2956 5860-5862 JJ denotes MA
T2957 5862-5863 -RRB- denotes )
T2958 5863-5872 NN denotes . Dextran
T2959 5873-5877 NN denotes T500
T2960 5878-5881 CC denotes and
T2961 5882-5889 NN denotes Percoll
T2962 5890-5894 VB denotes were
T2963 5895-5903 VB denotes received
T2964 5904-5908 IN denotes from
T2965 5909-5911 NNP denotes GE
T2966 5912-5931 NNP denotes Healthcare/Amersham
T2967 5932-5943 NNP denotes Biosciences
T2968 5944-5945 -LRB- denotes (
T2969 5945-5955 NNP denotes Piscataway
T2970 5955-5956 -COMMA- denotes ,
T2971 5957-5959 NNP denotes NJ
T2972 5959-5960 -RRB- denotes )
T2973 5962-5965 DT denotes The
T2974 5966-5979 JJ denotes enzyme-linked
T2975 5980-5993 JJ denotes immunosorbent
T2976 5994-5999 NN denotes assay
T2977 6000-6001 -LRB- denotes (
T2978 6001-6006 NN denotes ELISA
T2979 6006-6007 -RRB- denotes )
T2980 6008-6011 IN denotes for
T2981 6012-6017 NN denotes TNF-α
T2982 6018-6021 VB denotes was
T2983 6022-6030 VB denotes obtained
T2984 6031-6035 IN denotes from
T2985 6036-6046 NNP denotes Quantikine
T2986 6047-6048 NNP denotes R
T2987 6048-6049 CC denotes &
T2988 6049-6050 NNP denotes D
T2989 6051-6058 NNP denotes Systems
T2990 6059-6060 -LRB- denotes (
T2991 6060-6071 NNP denotes Minneapolis
T2992 6071-6072 -COMMA- denotes ,
T2993 6073-6075 NN denotes MN
T2994 6075-6076 -RRB- denotes )
T2995 6076-6090 NN denotes . Tris-glycine
T2996 6091-6094 CC denotes and
T2997 6095-6098 NN denotes DNA
T2998 6099-6110 NN denotes retardation
T2999 6111-6115 NN denotes gels
T3000 6115-6116 -COMMA- denotes ,
T3001 6117-6131 NN denotes nitrocellulose
T3002 6132-6141 NN denotes membranes
T3003 6141-6142 -COMMA- denotes ,
T3004 6143-6146 CC denotes and
T3005 6147-6154 JJ denotes western
T3006 6155-6159 NN denotes blot
T3007 6160-6167 VB denotes running
T3008 6168-6175 NN denotes buffers
T3009 6176-6180 VB denotes were
T3010 6181-6189 VB denotes obtained
T3011 6190-6194 IN denotes from
T3012 6195-6205 NN denotes Invitrogen
T3013 6206-6207 -LRB- denotes (
T3014 6207-6215 NNP denotes Carlsbad
T3015 6215-6216 -COMMA- denotes ,
T3016 6217-6219 NN denotes CA
T3017 6219-6220 -RRB- denotes )
T3018 6222-6237 JJ denotes NE-PER® nuclear
T3019 6238-6241 CC denotes and
T3020 6242-6253 JJ denotes cytoplasmic
T3021 6254-6264 NN denotes extraction
T3022 6265-6273 NN denotes reagents
T3023 6273-6274 -COMMA- denotes ,
T3024 6275-6278 DT denotes the
T3025 6279-6282 NN denotes BCA
T3026 6283-6290 NN denotes protein
T3027 6291-6298 NN denotes reagent
T3028 6299-6302 NN denotes kit
T3029 6302-6303 -COMMA- denotes ,
T3030 6304-6307 DT denotes the
T3031 6308-6319 NNP denotes Supersignal
T3032 6320-6324 NNP denotes West
T3033 6325-6329 NNP denotes Pico
T3034 6330-6346 NNP denotes Chemiluminescent
T3035 6347-6350 NNP denotes Kit
T3036 6350-6351 -COMMA- denotes ,
T3037 6352-6355 CC denotes and
T3038 6356-6359 DT denotes the
T3039 6360-6370 NNP denotes LightShift
T3040 6371-6387 NNP denotes Chemiluminescent
T3041 6388-6392 NNP denotes EMSA
T3042 6393-6396 NN denotes Kit
T3043 6397-6401 VB denotes were
T3044 6402-6411 VB denotes purchased
T3045 6412-6416 IN denotes from
T3046 6417-6423 NNP denotes Pierce
T3047 6424-6425 -LRB- denotes (
T3048 6425-6433 NNP denotes Rockland
T3049 6433-6434 -COMMA- denotes ,
T3050 6435-6437 NN denotes IL
T3051 6437-6438 -RRB- denotes )
T3052 6440-6444 NN denotes CREB
T3053 6445-6448 CC denotes and
T3054 6449-6454 NN denotes NF-κB
T3055 6455-6458 NN denotes DNA
T3056 6459-6474 NN denotes oligonucleotide
T3057 6475-6481 NN denotes probes
T3058 6482-6486 VB denotes were
T3059 6487-6498 VB denotes synthesized
T3060 6499-6501 IN denotes by
T3061 6502-6505 NN denotes IDT
T3062 6506-6507 -LRB- denotes (
T3063 6507-6517 NNP denotes Coralville
T3064 6517-6518 -COMMA- denotes ,
T3065 6519-6521 NNP denotes IA
T3066 6521-6522 -RRB- denotes )
T3067 6524-6529 NN denotes Nylon
T3068 6530-6539 NN denotes membranes
T3069 6540-6544 VB denotes were
T3070 6545-6554 VB denotes purchased
T3071 6555-6559 IN denotes from
T3072 6560-6565 NNP denotes Roche
T3073 6566-6573 NNP denotes Applied
T3074 6574-6582 NNP denotes Sciences
T3075 6583-6584 -LRB- denotes (
T3076 6584-6596 NNP denotes Indianapolis
T3077 6596-6597 -COMMA- denotes ,
T3078 6598-6600 NNP denotes IN
T3079 6600-6601 -RRB- denotes )
T3080 6603-6610 NN denotes Western
T3081 6611-6615 NN denotes blot
T3082 6616-6620 NN denotes band
T3083 6621-6635 NN denotes quantification
T3084 6636-6639 VB denotes was
T3085 6640-6649 VB denotes performed
T3086 6650-6654 IN denotes with
T3087 6655-6658 DT denotes the
T3088 6659-6669 NNP denotes UN-SCAN-IT
T3089 6670-6673 NNP denotes Gel
T3090 6674-6684 NNP denotes Digitizing
T3091 6685-6693 NN denotes software
T3092 6694-6695 -LRB- denotes (
T3093 6695-6699 NNP denotes Silk
T3094 6700-6710 NNP denotes Scientific
T3095 6710-6711 -COMMA- denotes ,
T3096 6712-6716 NNP denotes Orem
T3097 6716-6717 -COMMA- denotes ,
T3098 6718-6720 NN denotes UT
T3099 6720-6721 -RRB- denotes )
T3577 6724-6729 JJ denotes Human
T3578 6730-6740 NNP denotes Peripheral
T3579 6741-6746 NNP denotes Blood
T3580 6747-6758 NNP denotes Mononuclear
T3581 6759-6763 NNP denotes Cell
T3582 6764-6773 NN denotes Isolation
T3583 6774-6777 CC denotes and
T3584 6778-6789 NN denotes Stimulation
T3585 6790-6795 JJ denotes Human
T3586 6796-6807 JJ denotes mononuclear
T3587 6808-6813 NN denotes cells
T3588 6814-6818 VB denotes were
T3589 6819-6827 VB denotes isolated
T3590 6828-6832 IN denotes from
T3591 6833-6836 DT denotes the
T3592 6837-6847 JJ denotes peripheral
T3593 6848-6853 NN denotes blood
T3594 6854-6856 IN denotes of
T3595 6857-6861 CD denotes four
T3596 6862-6869 JJ denotes healthy
T3597 6870-6875 JJ denotes human
T3598 6876-6886 NN denotes volunteers
T3599 6888-6891 DT denotes The
T3600 6892-6898 NN denotes sample
T3601 6899-6903 NN denotes size
T3602 6904-6907 VB denotes was
T3603 6908-6914 VB denotes chosen
T3604 6915-6919 IN denotes from
T3605 6920-6923 PRP-DOLLAR- denotes our
T3606 6924-6934 RB denotes previously
T3607 6935-6944 VB denotes published
T3608 6945-6949 NN denotes work
T3609 6949-6950 -COMMA- denotes ,
T3610 6951-6956 WDT denotes which
T3611 6957-6969 VB denotes demonstrated
T3612 6970-6978 JJ denotes reliable
T3613 6979-6989 NN denotes results.10
T3614 6990-6994 NN denotes Cell
T3615 6995-7004 NN denotes isolation
T3616 7005-7008 CC denotes and
T3617 7009-7012 DT denotes all
T3618 7013-7023 JJ denotes subsequent
T3619 7024-7035 NN denotes experiments
T3620 7036-7040 VB denotes were
T3621 7041-7050 VB denotes conducted
T3622 7051-7056 IN denotes under
T3623 7057-7064 JJ denotes sterile
T3624 7065-7068 CC denotes and
T3625 7069-7081 JJ denotes pyrogen-free
T3626 7082-7092 NN denotes conditions
T3627 7094-7099 NN denotes Blood
T3628 7100-7109 VB denotes collected
T3629 7110-7112 IN denotes in
T3630 7113-7120 NN denotes heparin
T3631 7121-7126 NN denotes tubes
T3632 7127-7130 VB denotes was
T3633 7131-7140 VB denotes incubated
T3634 7141-7145 IN denotes with
T3635 7146-7153 NN denotes Dextran
T3636 7154-7158 NN denotes T500
T3637 7159-7162 CC denotes and
T3638 7163-7166 DT denotes the
T3639 7167-7170 JJ denotes red
T3640 7171-7176 NN denotes cells
T3641 7177-7181 VB denotes were
T3642 7182-7192 VB denotes sedimented
T3643 7193-7196 IN denotes for
T3644 7197-7199 CD denotes 40
T3645 7200-7207 NN denotes minutes
T3646 7208-7210 IN denotes at
T3647 7211-7215 NN denotes room
T3648 7216-7227 NN denotes temperature
T3649 7229-7232 DT denotes The
T3650 7233-7242 JJ denotes resultant
T3651 7243-7248 NN denotes serum
T3652 7249-7252 VB denotes was
T3653 7253-7257 RB denotes then
T3654 7258-7264 VB denotes washed
T3655 7265-7269 IN denotes with
T3656 7270-7274 NN denotes HBSS
T3657 7275-7278 CC denotes and
T3658 7279-7288 VB denotes separated
T3659 7289-7291 IN denotes by
T3660 7292-7299 NNP denotes Percoll
T3661 7300-7308 NN denotes gradient
T3662 7309-7323 NN denotes centrifugation
T3663 7324-7333 VB denotes according
T3664 7334-7336 TO denotes to
T3665 7337-7340 DT denotes the
T3666 7341-7355 NN denotes manufacturer’s
T3667 7356-7368 NN denotes instructions
T3668 7370-7374 NN denotes Cell
T3669 7375-7384 NN denotes viability
T3670 7385-7388 VB denotes was
T3671 7389-7397 VB denotes assessed
T3672 7398-7400 IN denotes by
T3673 7401-7407 NN denotes trypan
T3674 7408-7412 JJ denotes blue
T3675 7413-7416 NN denotes dye
T3676 7417-7426 NN denotes exclusion
T3677 7426-7427 -COMMA- denotes ,
T3678 7428-7432 IN denotes with
T3679 7433-7439 NN denotes purity
T3680 7440-7442 IN denotes of
T3681 7443-7450 JJ denotes greater
T3682 7451-7455 IN denotes than
T3683 7456-7458 CD denotes 95
T3684 7458-7459 NN denotes %
T3685 7461-7469 VB denotes Isolated
T3686 7470-7475 NN denotes cells
T3687 7476-7480 VB denotes were
T3688 7481-7492 VB denotes resuspended
T3689 7493-7495 IN denotes in
T3690 7496-7500 NN denotes RPMI
T3691 7501-7505 CD denotes 1640
T3692 7506-7518 VB denotes supplemented
T3693 7519-7523 IN denotes with
T3694 7524-7526 CD denotes 10
T3695 7526-7527 NN denotes %
T3696 7528-7531 NN denotes FBS
T3697 7532-7535 CC denotes and
T3698 7536-7537 CD denotes 5
T3699 7538-7540 NN denotes mM
T3700 7541-7546 NN denotes HEPES
T3701 7547-7549 IN denotes at
T3702 7550-7551 DT denotes a
T3703 7552-7565 NN denotes concentration
T3704 7566-7568 IN denotes of
T3705 7569-7570 CD denotes 1
T3706 7571-7572 SYM denotes x
T3707 7573-7584 NN denotes 107cells/mL
T3708 7586-7590 DT denotes Each
T3709 7591-7601 JJ denotes subsequent
T3710 7602-7612 NN denotes experiment
T3711 7613-7619 VB denotes listed
T3712 7620-7625 IN denotes below
T3713 7626-7629 VB denotes was
T3714 7630-7639 VB denotes conducted
T3715 7640-7642 IN denotes on
T3716 7643-7650 NN denotes samples
T3717 7651-7653 IN denotes of
T3718 7654-7655 CD denotes 5
T3719 7656-7657 NN denotes x
T3720 7658-7661 CD denotes 106
T3721 7662-7667 NN denotes cells
T3722 7668-7671 IN denotes per
T3723 7672-7681 NN denotes treatment
T3724 7682-7687 NN denotes group
T3725 7689-7697 VB denotes Isolated
T3726 7698-7703 NN denotes cells
T3727 7704-7708 VB denotes were
T3728 7709-7719 VB denotes stimulated
T3729 7720-7724 IN denotes with
T3730 7725-7731 CC denotes either
T3731 7732-7736 NN denotes HBSS
T3732 7737-7739 IN denotes as
T3733 7740-7741 DT denotes a
T3734 7742-7750 JJ denotes negative
T3735 7751-7758 NN denotes control
T3736 7758-7759 -COMMA- denotes ,
T3737 7760-7763 NN denotes LPS
T3738 7764-7765 -LRB- denotes (
T3739 7765-7766 CD denotes 1
T3740 7767-7772 NN denotes μg/mL
T3741 7772-7773 -RRB- denotes )
T3742 7773-7774 -COMMA- denotes ,
T3743 7775-7778 NN denotes PTX
T3744 7779-7780 -LRB- denotes (
T3745 7780-7782 CD denotes 20
T3746 7783-7785 NN denotes mM
T3747 7785-7786 -RRB- denotes )
T3748 7786-7787 -COMMA- denotes ,
T3749 7788-7790 CC denotes or
T3750 7791-7802 JJ denotes concomitant
T3751 7803-7806 NN denotes LPS
T3752 7807-7808 SYM denotes /
T3753 7809-7812 NN denotes PTX
T3754 7812-7813 -COMMA- denotes ,
T3755 7814-7816 IN denotes at
T3756 7817-7820 DT denotes the
T3757 7821-7836 JJ denotes above-mentioned
T3758 7837-7851 NN denotes concentrations
T3759 7851-7852 -COMMA- denotes ,
T3760 7853-7856 IN denotes for
T3761 7857-7859 CD denotes 30
T3762 7860-7867 NN denotes minutes
T3763 7868-7870 IN denotes at
T3764 7871-7875 NN denotes 37ºC
T3765 7877-7882 NN denotes Cells
T3766 7883-7887 VB denotes were
T3767 7888-7894 VB denotes placed
T3768 7895-7897 IN denotes on
T3769 7898-7901 NN denotes ice
T3770 7902-7905 IN denotes for
T3771 7906-7908 CD denotes 10
T3772 7909-7916 NN denotes minutes
T3773 7917-7919 TO denotes to
T3774 7920-7924 VB denotes stop
T3775 7925-7928 DT denotes the
T3776 7929-7937 NN denotes reaction
T3777 7938-7941 CC denotes and
T3778 7942-7945 DT denotes the
T3779 7946-7953 NN denotes samples
T3780 7954-7958 VB denotes were
T3781 7959-7963 RB denotes then
T3782 7964-7970 VB denotes stored
T3783 7971-7973 IN denotes at
T3784 7974-7979 NN denotes −70°C
T3785 7980-7983 IN denotes for
T3786 7984-7991 JJ denotes further
T3787 7992-8000 NN denotes analysis
T3788 8002-8005 DT denotes The
T3789 8006-8017 NN denotes stimulation
T3790 8018-8023 NN denotes times
T3791 8024-8027 CC denotes and
T3792 8028-8042 NN denotes concentrations
T3793 8043-8045 IN denotes of
T3794 8046-8049 NN denotes LPS
T3795 8050-8053 CC denotes and
T3796 8054-8057 NN denotes PTX
T3797 8058-8066 VB denotes utilized
T3798 8067-8069 IN denotes in
T3799 8070-8074 DT denotes this
T3800 8075-8080 NN denotes study
T3801 8081-8085 VB denotes were
T3802 8086-8096 VB denotes determined
T3803 8097-8099 IN denotes in
T3804 8100-8108 JJ denotes previous
T3805 8109-8114 NN denotes pilot
T3806 8115-8122 NN denotes studies
T3807 8123-8127 VB denotes done
T3808 8128-8130 IN denotes in
T3809 8131-8134 PRP-DOLLAR- denotes our
T3810 8135-8145 NN denotes laboratory
T3811 8145-8146 -COMMA- denotes ,
T3812 8147-8152 WDT denotes which
T3813 8153-8161 VB denotes examined
T3814 8162-8165 DT denotes the
T3815 8166-8173 NN denotes effects
T3816 8174-8176 IN denotes of
T3817 8177-8187 VB denotes increasing
T3818 8188-8202 NN denotes concentrations
T3819 8203-8205 IN denotes of
T3820 8206-8209 NN denotes PTX
T3821 8210-8212 IN denotes on
T3822 8213-8224 JJ denotes LPS-induced
T3823 8225-8230 NN denotes TNF-α
T3824 8231-8241 NN denotes production
T3825 8243-8246 NN denotes LPS
T3826 8247-8249 IN denotes at
T3827 8250-8251 DT denotes a
T3828 8252-8265 NN denotes concentration
T3829 8266-8268 IN denotes of
T3830 8269-8270 CD denotes 1
T3831 8271-8276 NN denotes μg/mL
T3832 8277-8280 CC denotes and
T3833 8281-8284 NN denotes PTX
T3834 8285-8287 IN denotes at
T3835 8288-8289 DT denotes a
T3836 8290-8303 NN denotes concentration
T3837 8304-8306 IN denotes of
T3838 8307-8309 CD denotes 20
T3839 8310-8312 NN denotes mM
T3840 8313-8317 VB denotes were
T3841 8318-8321 DT denotes the
T3842 8322-8329 JJ denotes minimum
T3843 8330-8344 NN denotes concentrations
T3844 8345-8354 JJ denotes necessary
T3845 8355-8357 TO denotes to
T3846 8358-8365 VB denotes produce
T3847 8366-8367 DT denotes a
T3848 8368-8380 JJ denotes reproducible
T3849 8381-8384 CC denotes and
T3850 8385-8393 JJ denotes reliable
T3851 8394-8407 NN denotes up-regulation
T3852 8408-8411 CC denotes and
T3853 8412-8427 NN denotes down-regulation
T3854 8428-8430 IN denotes in
T3855 8431-8436 NN denotes TNF-α
T3856 8437-8447 NN denotes production
T3857 8447-8448 -COMMA- denotes ,
T3858 8449-8461 RB denotes respectively
T3859 8461-8462 -COMMA- denotes ,
T3860 8463-8465 IN denotes in
T3861 8466-8478 JJ denotes quantitative
T3862 8479-8485 NN denotes assays
T3863 8486-8487 -LRB- denotes (
T3864 8487-8491 NN denotes data
T3865 8492-8495 RB denotes not
T3866 8496-8501 VB denotes shown
T3867 8501-8502 -RRB- denotes )
T4023 8505-8511 NN denotes Effect
T4024 8512-8514 IN denotes of
T4025 8515-8518 NN denotes PTX
T4027 8522-8533 JJ denotes LPS-induced
T4028 8534-8539 NN denotes TNF-α
T4029 8540-8550 NN denotes production
T4030 8551-8553 IN denotes by
T4031 8554-8565 JJ denotes mononuclear
T4032 8566-8571 NN denotes cells
T4033 8572-8575 DT denotes The
T4034 8576-8589 NN denotes concentration
T4035 8590-8592 IN denotes of
T4036 8593-8598 NN denotes TNF-α
T4037 8599-8602 VB denotes was
T4038 8603-8617 RB denotes quantitatively
T4039 8618-8628 VB denotes determined
T4040 8629-8631 IN denotes by
T4041 8632-8637 NN denotes ELISA
T4042 8638-8640 IN denotes in
T4043 8641-8644 DT denotes the
T4044 8645-8650 NN denotes media
T4045 8651-8653 IN denotes of
T4046 8654-8659 NN denotes cells
T4047 8660-8667 VB denotes exposed
T4049 8671-8674 DT denotes the
T4050 8675-8685 NN denotes treatments
T4051 8686-8695 VB denotes described
T4052 8696-8701 IN denotes above
T4053 8702-8707 IN denotes after
T4054 8708-8719 NN denotes stimulation
T4055 8720-8723 IN denotes for
T4056 8724-8726 RB denotes up
T4057 8727-8729 TO denotes to
T4058 8730-8731 DT denotes a
T4059 8732-8739 NN denotes maximum
T4060 8740-8742 IN denotes of
T4061 8743-8745 CD denotes 18
T4062 8746-8751 NN denotes hours
T4063 8751-8752 -COMMA- denotes ,
T4064 8753-8755 IN denotes at
T4065 8756-8762 JJ denotes 3-hour
T4066 8763-8772 NN denotes intervals
T4067 8774-8777 DT denotes The
T4068 8778-8785 NN denotes results
T4069 8786-8789 VB denotes are
T4070 8790-8799 VB denotes expressed
T4071 8800-8802 IN denotes in
T4072 8803-8808 NN denotes pg/mL
T4475 8811-8819 NN denotes SDS-PAGE
T4476 8820-8823 CC denotes and
T4477 8824-8838 NN denotes Immunoblotting
T4478 8839-8840 -LRB- denotes (
T4479 8840-8845 NN denotes I-κBα
T4480 8845-8846 -COMMA- denotes ,
T4481 8847-8852 NN denotes NF-κB
T4482 8853-8856 NN denotes p65
T4483 8856-8857 -COMMA- denotes ,
T4484 8858-8861 CC denotes and
T4485 8862-8866 NN denotes CREB
T4486 8866-8867 -RRB- denotes )
T4487 8868-8877 VB denotes Following
T4488 8878-8889 NN denotes stimulation
T4489 8889-8890 -COMMA- denotes ,
T4490 8891-8896 NN denotes PBMCs
T4491 8897-8901 VB denotes were
T4492 8902-8908 VB denotes washed
T4493 8909-8913 IN denotes with
T4494 8914-8922 JJ denotes ice-cold
T4495 8923-8926 NN denotes PBS
T4496 8927-8930 CC denotes and
T4497 8931-8942 VB denotes centrifuged
T4498 8943-8945 TO denotes to
T4499 8946-8953 VB denotes collect
T4500 8954-8955 DT denotes a
T4501 8956-8960 NN denotes cell
T4502 8961-8967 NN denotes pellet
T4503 8969-8972 DT denotes The
T4504 8973-8979 NN denotes pellet
T4505 8980-8983 VB denotes was
T4506 8984-8995 VB denotes resuspended
T4507 8996-8998 IN denotes in
T4508 8999-9007 JJ denotes ice-cold
T4509 9008-9011 NN denotes SDS
T4510 9012-9018 NN denotes sample
T4511 9019-9025 NN denotes buffer
T4512 9026-9038 VB denotes supplemented
T4513 9039-9043 IN denotes with
T4514 9044-9047 CD denotes 100
T4515 9048-9050 NN denotes mM
T4516 9051-9065 NN denotes dithiothreitol
T4517 9067-9078 JJ denotes Cytoplasmic
T4518 9079-9082 CC denotes and
T4519 9083-9090 JJ denotes nuclear
T4520 9091-9099 NN denotes extracts
T4521 9100-9104 VB denotes were
T4522 9105-9113 VB denotes isolated
T4523 9114-9118 IN denotes with
T4524 9119-9125 NN denotes NE-PER
T4525 9126-9133 JJ denotes nuclear
T4526 9134-9137 CC denotes and
T4527 9138-9149 JJ denotes cytoplasmic
T4528 9150-9160 NN denotes extraction
T4529 9161-9169 NN denotes reagents
T4530 9170-9174 IN denotes with
T4531 9175-9177 NN denotes 1x
T4532 9178-9182 NNP denotes Halt
T4533 9183-9191 NNP denotes Protease
T4534 9192-9201 NNP denotes Inhibitor
T4535 9202-9210 NNP denotes Cocktail
T4536 9211-9220 VB denotes according
T4537 9221-9223 TO denotes to
T4538 9224-9227 DT denotes the
T4539 9228-9242 NN denotes manufacturer’s
T4540 9243-9255 NN denotes instructions
T4541 9257-9264 NN denotes Protein
T4542 9265-9279 NN denotes concentrations
T4543 9280-9284 VB denotes were
T4544 9285-9295 VB denotes determined
T4545 9296-9300 IN denotes with
T4546 9301-9304 DT denotes the
T4547 9305-9308 NN denotes BCA
T4548 9309-9316 NN denotes protein
T4549 9317-9324 NN denotes reagent
T4550 9325-9328 NN denotes kit
T4551 9329-9332 IN denotes for
T4552 9333-9337 DT denotes each
T4553 9338-9344 NN denotes sample
T4554 9345-9354 VB denotes according
T4555 9355-9357 TO denotes to
T4556 9358-9359 DT denotes a
T4557 9360-9372 JJ denotes standardized
T4558 9373-9378 NN denotes curve
T4559 9379-9382 IN denotes for
T4560 9383-9390 NN denotes albumin
T4561 9392-9396 NN denotes Cell
T4562 9397-9404 NN denotes lysates
T4563 9405-9409 VB denotes were
T4564 9410-9419 VB denotes collected
T4565 9420-9422 IN denotes by
T4566 9423-9430 VB denotes boiling
T4567 9431-9434 DT denotes the
T4568 9435-9442 NN denotes samples
T4569 9443-9445 IN denotes in
T4570 9446-9447 DT denotes a
T4571 9448-9453 NN denotes 100ºC
T4572 9454-9459 NN denotes water
T4573 9460-9464 NN denotes bath
T4574 9465-9468 IN denotes for
T4575 9469-9470 CD denotes 5
T4576 9471-9478 NN denotes minutes
T4577 9480-9483 CD denotes Ten
T4578 9484-9486 NN denotes μg
T4579 9487-9489 IN denotes of
T4580 9490-9497 NN denotes protein
T4581 9498-9501 IN denotes per
T4582 9502-9508 NN denotes sample
T4583 9509-9512 VB denotes was
T4584 9513-9522 VB denotes separated
T4585 9523-9525 IN denotes by
T4586 9526-9544 NN denotes SDS-polyacrylamide
T4587 9545-9548 NN denotes gel
T4588 9549-9564 NN denotes electrophoresis
T4589 9565-9572 IN denotes through
T4590 9573-9577 CD denotes 8–16
T4591 9577-9578 NN denotes %
T4592 9579-9591 NN denotes tris-glycine
T4593 9592-9606 NN denotes polyacrylamide
T4594 9607-9615 NN denotes gradient
T4595 9616-9620 NN denotes gels
T4596 9621-9624 CC denotes and
T4597 9625-9636 VB denotes transferred
T4598 9637-9639 TO denotes to
T4599 9640-9654 VB denotes nitrocellulose
T4600 9655-9664 NN denotes membranes
T4601 9666-9669 DT denotes The
T4602 9670-9679 NN denotes membranes
T4603 9680-9684 VB denotes were
T4604 9685-9692 VB denotes blocked
T4605 9693-9697 IN denotes with
T4606 9698-9699 CD denotes 5
T4607 9699-9700 NN denotes %
T4608 9701-9705 NN denotes milk
T4609 9706-9708 IN denotes in
T4610 9709-9722 JJ denotes Tris-buffered
T4611 9723-9735 NN denotes saline/Tween
T4612 9736-9738 CD denotes 20
T4613 9739-9740 -LRB- denotes (
T4614 9740-9747 NNP denotes Fischer
T4615 9748-9758 NNP denotes Scientific
T4616 9758-9759 -COMMA- denotes ,
T4617 9760-9770 NNP denotes Pittsburgh
T4618 9770-9771 -COMMA- denotes ,
T4619 9772-9774 NNP denotes PA
T4620 9774-9775 -RRB- denotes )
T4621 9776-9779 IN denotes for
T4622 9780-9781 CD denotes 1
T4623 9782-9786 NN denotes hour
T4624 9788-9799 JJ denotes Cytoplasmic
T4625 9800-9808 NN denotes extracts
T4626 9809-9813 VB denotes were
T4627 9814-9823 VB denotes incubated
T4628 9824-9828 IN denotes with
T4629 9829-9843 VB denotes phosphorylated
T4630 9844-9849 JJ denotes I-κBα
T4631 9850-9858 NN denotes antibody
T4632 9859-9860 -LRB- denotes (
T4633 9860-9861 CD denotes 1
T4634 9861-9862 -COLON- denotes :
T4635 9862-9865 CD denotes 200
T4636 9865-9866 -RRB- denotes )
T4637 9866-9867 -COMMA- denotes ,
T4638 9868-9873 IN denotes while
T4639 9874-9881 JJ denotes nuclear
T4640 9882-9890 NN denotes extracts
T4641 9891-9895 VB denotes were
T4642 9896-9905 VB denotes incubated
T4643 9906-9910 IN denotes with
T4644 9911-9917 CC denotes either
T4645 9918-9932 VB denotes phosphorylated
T4646 9933-9938 NN denotes NF-κB
T4647 9939-9942 NN denotes p65
T4648 9943-9951 NN denotes antibody
T4649 9952-9953 -LRB- denotes (
T4650 9953-9954 CD denotes 1
T4651 9954-9955 -COLON- denotes :
T4652 9955-9958 CD denotes 500
T4653 9958-9959 -RRB- denotes )
T4654 9960-9962 CC denotes or
T4655 9963-9977 VB denotes phosphorylated
T4656 9978-9982 NN denotes CREB
T4657 9983-9991 NN denotes antibody
T4658 9992-9993 -LRB- denotes (
T4659 9993-9994 CD denotes 1
T4660 9994-9995 -COLON- denotes :
T4661 9995-9998 CD denotes 500
T4662 9998-9999 -RRB- denotes )
T4663 10000-10009 RB denotes overnight
T4664 10010-10012 IN denotes at
T4665 10013-10016 NN denotes 4°C
T4666 10017-10019 IN denotes in
T4667 10020-10028 JJ denotes separate
T4668 10029-10040 NN denotes experiments
T4669 10042-10045 DT denotes The
T4670 10046-10055 NN denotes membranes
T4671 10056-10060 VB denotes were
T4672 10061-10067 VB denotes washed
T4673 10068-10072 IN denotes with
T4674 10073-10086 JJ denotes Tris-buffered
T4675 10087-10099 NN denotes saline/Tween
T4676 10100-10102 CD denotes 20
T4677 10103-10106 CC denotes and
T4678 10107-10116 VB denotes incubated
T4679 10117-10120 IN denotes for
T4680 10121-10122 CD denotes 1
T4681 10123-10127 NN denotes hour
T4682 10128-10130 IN denotes at
T4683 10131-10135 NN denotes room
T4684 10136-10147 NN denotes temperature
T4685 10148-10152 IN denotes with
T4686 10153-10156 DT denotes the
T4687 10157-10166 JJ denotes secondary
T4688 10167-10175 NN denotes antibody
T4689 10175-10176 -COMMA- denotes ,
T4690 10177-10188 JJ denotes horseradish
T4691 10189-10206 JJ denotes peroxidase-linked
T4692 10207-10218 JJ denotes anti-rabbit
T4693 10219-10222 NN denotes IgG
T4694 10223-10230 VB denotes diluted
T4695 10231-10232 CD denotes 1
T4696 10232-10233 -COLON- denotes :
T4697 10233-10237 CD denotes 2000
T4698 10238-10240 IN denotes in
T4699 10241-10249 VB denotes blocking
T4700 10250-10258 NN denotes solution
T4701 10260-10265 IN denotes After
T4702 10266-10274 VB denotes repeated
T4703 10275-10282 NN denotes washing
T4704 10283-10285 IN denotes of
T4705 10286-10289 DT denotes the
T4706 10290-10298 NN denotes membrane
T4707 10298-10299 -COMMA- denotes ,
T4708 10300-10303 DT denotes the
T4709 10304-10315 NNP denotes Supersignal
T4710 10316-10320 NNP denotes West
T4711 10321-10325 NNP denotes Pico
T4712 10326-10342 NNP denotes Chemiluminescent
T4713 10343-10346 NNP denotes Kit
T4714 10347-10350 VB denotes was
T4715 10351-10358 VB denotes applied
T4716 10359-10362 IN denotes for
T4717 10363-10371 NN denotes antibody
T4718 10372-10381 NN denotes detection
T4719 10382-10385 IN denotes per
T4720 10386-10389 DT denotes the
T4721 10390-10404 NN denotes manufacturer’s
T4722 10405-10417 NN denotes instructions
T4723 10419-10426 JJ denotes Western
T4724 10427-10431 NN denotes blot
T4725 10432-10436 NN denotes data
T4726 10437-10439 VB denotes is
T4727 10440-10449 VB denotes presented
T4728 10450-10452 IN denotes as
T4729 10453-10454 DT denotes a
T4730 10455-10465 NN denotes percentage
T4731 10466-10468 IN denotes of
T4732 10469-10472 NN denotes LPS
T4733 10473-10484 NN denotes stimulation
T4734 10486-10489 DT denotes The
T4735 10490-10500 NN denotes percentage
T4736 10501-10503 IN denotes of
T4737 10504-10507 NN denotes LPS
T4738 10508-10519 NN denotes stimulation
T4739 10520-10523 VB denotes was
T4740 10524-10534 VB denotes calculated
T4741 10535-10537 IN denotes by
T4742 10538-10546 VB denotes dividing
T4743 10547-10550 DT denotes the
T4744 10551-10555 JJ denotes mean
T4745 10556-10560 NN denotes band
T4746 10561-10566 NN denotes pixel
T4747 10567-10572 NN denotes total
T4748 10573-10576 IN denotes for
T4749 10577-10581 DT denotes each
T4750 10582-10591 NN denotes treatment
T4751 10592-10595 NN denotes arm
T4752 10596-10603 VB denotes divided
T4753 10604-10606 IN denotes by
T4754 10607-10610 DT denotes the
T4755 10611-10615 JJ denotes mean
T4756 10616-10621 NN denotes pixel
T4757 10622-10627 NN denotes total
T4758 10628-10630 IN denotes of
T4759 10631-10638 NN denotes samples
T4760 10639-10649 VB denotes stimulated
T4761 10650-10654 IN denotes with
T4762 10655-10658 NN denotes LPS
T4763 10659-10662 CC denotes and
T4764 10663-10674 VB denotes multiplying
T4765 10675-10677 IN denotes by
T4766 10678-10681 CD denotes 100
T4767 10683-10687 RB denotes Thus
T4768 10687-10688 -COMMA- denotes ,
T4769 10689-10692 NN denotes LPS
T4770 10693-10704 NN denotes stimulation
T4771 10705-10707 VB denotes is
T4772 10708-10716 VB denotes reported
T4773 10717-10719 IN denotes as
T4774 10720-10723 CD denotes 100
T4775 10723-10724 NN denotes %
T4776 10725-10728 CC denotes and
T4777 10729-10733 DT denotes each
T4778 10734-10736 IN denotes of
T4779 10737-10740 DT denotes the
T4780 10741-10750 NN denotes treatment
T4781 10751-10755 NN denotes arms
T4782 10756-10758 VB denotes is
T4783 10759-10767 VB denotes reported
T4784 10768-10770 IN denotes as
T4785 10771-10772 DT denotes a
T4786 10773-10780 NN denotes percent
T4787 10781-10783 IN denotes of
T4788 10784-10787 NN denotes LPS
T4789 10788-10799 NN denotes stimulation
T5231 10802-10807 NN denotes NF-κB
T5232 10808-10811 CC denotes and
T5233 10812-10816 NN denotes CREB
T5234 10817-10832 JJ denotes Electrophoretic
T5235 10833-10841 NN denotes Mobility
T5236 10842-10847 NN denotes Shift
T5237 10848-10853 NN denotes Assay
T5238 10854-10855 -LRB- denotes (
T5239 10855-10859 NN denotes EMSA
T5240 10859-10860 -RRB- denotes )
T5241 10861-10864 DT denotes The
T5242 10865-10880 JJ denotes non-radioactive
T5243 10881-10891 NNP denotes LightShift
T5244 10892-10908 NNP denotes Chemiluminescent
T5245 10909-10913 NNP denotes EMSA
T5246 10914-10917 NN denotes Kit
T5247 10918-10921 VB denotes was
T5248 10922-10926 VB denotes used
T5249 10927-10929 TO denotes to
T5250 10930-10936 VB denotes detect
T5251 10937-10948 JJ denotes DNA-protein
T5252 10949-10961 NN denotes interactions
T5253 10963-10966 DT denotes The
T5254 10967-10972 NN denotes NF-κB
T5255 10973-10975 NN denotes 3′
T5256 10976-10982 NN denotes biotin
T5257 10983-10994 JJ denotes end-labeled
T5258 10995-10998 NN denotes DNA
T5259 10999-11014 NN denotes oligonucleotide
T5260 11015-11019 VB denotes used
T5261 11020-11022 IN denotes as
T5262 11023-11024 DT denotes a
T5263 11025-11030 NN denotes probe
T5264 11031-11034 IN denotes for
T5265 11035-11038 DT denotes the
T5266 11039-11043 NN denotes EMSA
T5267 11044-11047 VB denotes was
T5268 11048-11049 DT denotes a
T5269 11050-11055 JJ denotes 42-bp
T5270 11056-11062 JJ denotes double
T5271 11063-11071 JJ denotes stranded
T5272 11072-11081 NN denotes construct
T5273 11082-11083 -LRB- denotes (
T5274 11083-11129 NN denotes 5′-TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
T5275 11130-11131 NN denotes –
T5276 11132-11134 NN denotes 3′
T5277 11134-11135 -RRB- denotes )
T5278 11136-11146 VB denotes containing
T5279 11147-11150 CD denotes two
T5280 11151-11159 RB denotes tandemly
T5281 11160-11168 VB denotes repeated
T5282 11169-11174 NN denotes NF-κB
T5283 11175-11182 NN denotes binding
T5284 11183-11188 NN denotes sites
T5285 11189-11190 -LRB- denotes (
T5286 11190-11200 VB denotes underlined
T5287 11200-11201 -RRB- denotes )
T5288 11203-11214 NN denotes Specificity
T5289 11215-11218 VB denotes was
T5290 11219-11229 VB denotes determined
T5291 11230-11232 IN denotes by
T5292 11233-11234 DT denotes a
T5293 11235-11246 NN denotes competition
T5294 11247-11252 NN denotes assay
T5295 11253-11257 IN denotes with
T5296 11258-11261 DT denotes the
T5297 11262-11270 NN denotes addition
T5298 11271-11273 IN denotes of
T5299 11274-11277 CD denotes 200
T5300 11278-11283 JJ denotes molar
T5301 11284-11290 NN denotes excess
T5302 11291-11293 IN denotes of
T5303 11294-11303 JJ denotes unlabeled
T5304 11304-11310 JJ denotes double
T5305 11311-11319 JJ denotes stranded
T5306 11320-11325 NN denotes NF-κB
T5307 11326-11341 NN denotes oligonucleotide
T5308 11343-11346 DT denotes The
T5309 11347-11351 NN denotes CREB
T5310 11352-11354 NN denotes 3′
T5311 11355-11361 NN denotes biotin
T5312 11362-11373 JJ denotes end-labeled
T5313 11374-11377 NN denotes DNA
T5314 11378-11393 NN denotes oligonucleotide
T5315 11394-11398 VB denotes used
T5316 11399-11401 IN denotes as
T5317 11402-11403 DT denotes a
T5318 11404-11409 NN denotes probe
T5319 11410-11413 IN denotes for
T5320 11414-11417 DT denotes the
T5321 11418-11422 NN denotes EMSA
T5322 11423-11426 VB denotes was
T5323 11427-11428 DT denotes a
T5324 11429-11434 JJ denotes 23-bp
T5325 11435-11441 JJ denotes double
T5326 11442-11450 JJ denotes stranded
T5327 11451-11460 NN denotes construct
T5328 11461-11462 -LRB- denotes (
T5329 11462-11468 NN denotes 5′-TTT
T5330 11469-11472 NN denotes TCG
T5331 11473-11476 NN denotes AGC
T5332 11477-11492 NN denotes TCTGACGTCAGA-GC
T5333 11493-11494 NN denotes –
T5334 11495-11497 NN denotes 3′
T5335 11497-11498 -RRB- denotes )
T5336 11499-11509 VB denotes containing
T5337 11510-11513 DT denotes the
T5338 11514-11517 NN denotes CRE
T5339 11518-11527 NN denotes consensus
T5340 11528-11536 NN denotes sequence
T5341 11537-11538 -LRB- denotes (
T5342 11538-11548 VB denotes underlined
T5343 11548-11549 -RRB- denotes )
T5344 11551-11562 NN denotes Specificity
T5345 11563-11566 VB denotes was
T5346 11567-11577 VB denotes determined
T5347 11578-11580 IN denotes by
T5348 11581-11582 DT denotes a
T5349 11583-11594 NN denotes competition
T5350 11595-11600 NN denotes assay
T5351 11601-11605 IN denotes with
T5352 11606-11609 DT denotes the
T5353 11610-11618 NN denotes addition
T5354 11619-11621 IN denotes of
T5355 11622-11625 CD denotes 200
T5356 11626-11631 JJ denotes molar
T5357 11632-11638 NN denotes excess
T5358 11639-11641 IN denotes of
T5359 11642-11651 JJ denotes unlabeled
T5360 11652-11658 JJ denotes double
T5361 11659-11667 JJ denotes stranded
T5362 11668-11672 NN denotes CREB
T5363 11673-11688 NN denotes oligonucleotide
T5364 11690-11697 JJ denotes Nuclear
T5365 11698-11706 NN denotes extracts
T5366 11707-11708 -LRB- denotes (
T5367 11708-11710 CD denotes 10
T5368 11711-11713 NN denotes μg
T5369 11713-11714 -RRB- denotes )
T5370 11715-11719 VB denotes were
T5371 11720-11729 VB denotes incubated
T5372 11730-11734 IN denotes with
T5373 11735-11736 CD denotes 5
T5374 11737-11739 NN denotes nM
T5375 11740-11745 NN denotes NF-κB
T5376 11746-11748 CC denotes or
T5377 11749-11753 NN denotes CREB
T5378 11754-11759 NN denotes probe
T5379 11760-11761 -LRB- denotes (
T5380 11761-11766 NN denotes NF-kB
T5381 11766-11767 -COLON- denotes :
T5382 11768-11770 NN denotes 1x
T5383 11771-11778 NN denotes binding
T5384 11779-11785 NN denotes buffer
T5385 11785-11786 -COMMA- denotes ,
T5386 11787-11789 CD denotes 50
T5387 11790-11792 NN denotes mM
T5388 11793-11796 NN denotes KCl
T5389 11796-11797 -COMMA- denotes ,
T5390 11798-11799 CD denotes 1
T5391 11800-11802 NN denotes mM
T5392 11803-11807 NN denotes EDTA
T5393 11807-11808 -COMMA- denotes ,
T5394 11809-11810 CD denotes 1
T5395 11811-11813 NN denotes mM
T5396 11814-11817 NN denotes DTT
T5397 11817-11818 -COMMA- denotes ,
T5398 11819-11822 CD denotes 0.1
T5399 11822-11823 NN denotes %
T5400 11824-11828 NN denotes NP40
T5401 11828-11829 -COMMA- denotes ,
T5402 11830-11832 CD denotes 10
T5403 11832-11833 NN denotes %
T5404 11834-11842 NN denotes glycerol
T5405 11842-11843 -COMMA- denotes ,
T5406 11844-11847 CC denotes and
T5407 11848-11850 CD denotes 50
T5408 11851-11856 NN denotes ng/μl
T5409 11857-11861 NN denotes poly
T5410 11862-11867 NN denotes dI-dC
T5411 11867-11868 -COMMA- denotes ,
T5412 11869-11873 NN denotes CREB
T5413 11873-11874 -COLON- denotes :
T5414 11875-11877 NN denotes 1x
T5415 11878-11885 NN denotes binding
T5416 11886-11892 NN denotes buffer
T5417 11892-11893 -COMMA- denotes ,
T5418 11894-11896 CD denotes 20
T5419 11897-11899 NN denotes mM
T5420 11900-11904 NN denotes Tris
T5421 11904-11905 -COMMA- denotes ,
T5422 11906-11908 NN denotes pH
T5423 11909-11912 CD denotes 7.5
T5424 11912-11913 -COMMA- denotes ,
T5425 11914-11916 CD denotes 50
T5426 11917-11919 NN denotes mM
T5427 11920-11923 NN denotes KCl
T5428 11923-11924 -COMMA- denotes ,
T5429 11925-11926 CD denotes 1
T5430 11927-11929 NN denotes mM
T5431 11930-11934 NN denotes EDTA
T5432 11934-11935 -COMMA- denotes ,
T5433 11936-11937 CD denotes 1
T5434 11938-11940 NN denotes mM
T5435 11941-11944 NN denotes DTT
T5436 11944-11945 -COMMA- denotes ,
T5437 11946-11950 CD denotes 0.10
T5438 11950-11951 NN denotes %
T5439 11952-11956 NN denotes NP40
T5440 11956-11957 -COMMA- denotes ,
T5441 11958-11959 CD denotes 6
T5442 11959-11960 NN denotes %
T5443 11961-11969 NN denotes glycerol
T5444 11969-11970 -COMMA- denotes ,
T5445 11971-11974 CD denotes 0.1
T5446 11975-11980 NN denotes mg/mL
T5447 11981-11984 NN denotes BSA
T5448 11985-11988 CC denotes and
T5449 11989-11991 CD denotes 50
T5450 11992-11997 NN denotes ng/μl
T5451 11998-12002 NN denotes poly
T5452 12003-12008 NN denotes dI-dC
T5453 12008-12009 -RRB- denotes )
T5454 12010-12013 CC denotes and
T5455 12014-12018 VB denotes were
T5456 12019-12023 RB denotes then
T5457 12024-12039 VB denotes electrophoresed
T5458 12040-12047 IN denotes through
T5459 12048-12049 DT denotes a
T5460 12050-12051 CD denotes 6
T5461 12051-12052 NN denotes %
T5462 12053-12056 NN denotes DNA
T5463 12057-12068 NN denotes retardation
T5464 12069-12072 NN denotes gel
T5465 12073-12075 IN denotes at
T5466 12076-12080 NN denotes 100V
T5467 12081-12084 IN denotes for
T5468 12085-12087 CD denotes 90
T5469 12088-12095 NN denotes minutes
T5470 12097-12100 DT denotes The
T5471 12101-12105 NN denotes gels
T5472 12106-12110 VB denotes were
T5473 12111-12130 RB denotes electrophoretically
T5474 12131-12142 VB denotes transferred
T5475 12143-12145 IN denotes at
T5476 12146-12151 NN denotes 380mA
T5477 12152-12155 IN denotes for
T5478 12156-12157 CD denotes 1
T5479 12158-12162 NN denotes hour
T5480 12163-12165 IN denotes on
T5481 12166-12169 NN denotes ice
T5482 12170-12172 TO denotes to
T5483 12173-12174 DT denotes a
T5484 12175-12185 RB denotes positively
T5485 12186-12193 VB denotes charged
T5486 12194-12199 NN denotes nylon
T5487 12200-12208 NN denotes membrane
T5488 12209-12212 CC denotes and
T5489 12213-12224 RB denotes immediately
T5490 12225-12237 JJ denotes cross-linked
T5491 12238-12241 IN denotes for
T5492 12242-12244 CD denotes 15
T5493 12245-12252 NN denotes minutes
T5494 12253-12257 IN denotes with
T5495 12258-12259 DT denotes a
T5496 12260-12262 NN denotes UV
T5497 12263-12279 NN denotes transilluminator
T5498 12280-12288 VB denotes equipped
T5499 12289-12293 IN denotes with
T5500 12294-12295 DT denotes a
T5501 12296-12299 CD denotes 312
T5502 12300-12302 NN denotes nm
T5503 12303-12307 NN denotes bulb
T5504 12309-12333 JJ denotes Streptavidin-horseradish
T5505 12334-12344 NN denotes peroxidase
T5506 12345-12354 NN denotes conjugate
T5507 12355-12358 CC denotes and
T5508 12359-12362 DT denotes the
T5509 12363-12373 NNP denotes LightShift
T5510 12374-12390 NNP denotes Chemiluminescent
T5511 12391-12400 NNP denotes Substrate
T5512 12401-12405 VB denotes were
T5513 12406-12410 VB denotes used
T5514 12411-12413 TO denotes to
T5515 12414-12420 VB denotes detect
T5516 12421-12424 DT denotes the
T5517 12425-12431 NN denotes biotin
T5518 12432-12443 JJ denotes end-labeled
T5519 12444-12447 NN denotes DNA
T5520 12449-12452 DT denotes The
T5521 12453-12458 NN denotes nylon
T5522 12459-12468 NN denotes membranes
T5523 12469-12473 VB denotes were
T5524 12474-12481 VB denotes exposed
T5525 12482-12484 TO denotes to
T5526 12485-12490 NN denotes x-ray
T5527 12491-12495 NN denotes film
T5528 12496-12499 IN denotes for
T5529 12500-12503 CD denotes 1–3
T5530 12504-12511 NN denotes minutes
T5531 12512-12515 IN denotes for
T5532 12516-12525 NN denotes detection
T5799 12528-12532 NN denotes Role
T5800 12533-12535 IN denotes of
T5801 12536-12539 NN denotes PKA
T5802 12540-12542 IN denotes on
T5803 12543-12554 JJ denotes LPS-induced
T5804 12555-12566 JJ denotes mononuclear
T5805 12567-12571 NN denotes cell
T5806 12572-12577 NN denotes TNF-α
T5807 12578-12588 NN denotes production
T5808 12589-12591 TO denotes To
T5809 12592-12600 VB denotes evaluate
T5810 12601-12604 DT denotes the
T5811 12605-12609 NN denotes role
T5812 12610-12612 IN denotes of
T5813 12613-12616 NN denotes PKA
T5814 12617-12619 IN denotes on
T5815 12620-12625 NN denotes TNF-α
T5816 12626-12636 NN denotes production
T5817 12637-12639 IN denotes in
T5818 12640-12651 JJ denotes mononuclear
T5819 12652-12657 NN denotes cells
T5820 12658-12661 CC denotes and
T5821 12662-12665 PRP-DOLLAR- denotes its
T5822 12666-12677 NN denotes involvement
T5823 12678-12680 IN denotes in
T5824 12681-12684 DT denotes the
T5825 12685-12696 NN denotes attenuation
T5826 12697-12699 IN denotes of
T5827 12700-12711 JJ denotes LPS-induced
T5828 12712-12717 NN denotes TNF-α
T5829 12718-12728 NN denotes production
T5830 12729-12737 VB denotes observed
T5831 12738-12747 VB denotes following
T5832 12748-12751 NN denotes PTX
T5833 12752-12761 NN denotes treatment
T5834 12761-12762 -COMMA- denotes ,
T5835 12763-12766 NN denotes H89
T5836 12766-12767 -COMMA- denotes ,
T5837 12768-12769 DT denotes a
T5838 12770-12778 JJ denotes specific
T5839 12779-12782 NN denotes PKA
T5840 12783-12792 NN denotes inhibitor
T5841 12792-12793 -COMMA- denotes ,
T5842 12794-12797 VB denotes was
T5843 12798-12806 VB denotes utilized
T5844 12808-12816 VB denotes Isolated
T5845 12817-12828 JJ denotes mononuclear
T5846 12829-12834 NN denotes cells
T5847 12835-12839 VB denotes were
T5848 12840-12849 VB denotes incubated
T5849 12850-12859 VB denotes according
T5850 12860-12862 TO denotes to
T5851 12863-12866 DT denotes the
T5852 12867-12876 NN denotes treatment
T5853 12877-12883 NN denotes groups
T5854 12884-12893 VB denotes described
T5855 12894-12899 RB denotes above
T5856 12900-12902 IN denotes in
T5857 12903-12906 DT denotes the
T5858 12907-12915 NN denotes presence
T5859 12916-12919 CC denotes and
T5860 12920-12927 NN denotes absence
T5861 12928-12930 IN denotes of
T5862 12931-12943 NN denotes pretreatment
T5863 12944-12948 IN denotes with
T5864 12949-12952 NN denotes H89
T5865 12953-12954 -LRB- denotes (
T5866 12954-12956 CD denotes 10
T5867 12957-12959 NN denotes μM
T5868 12959-12960 -RRB- denotes )
T5869 12961-12964 IN denotes for
T5870 12965-12966 CD denotes 1
T5871 12967-12971 NN denotes hour
T5872 12972-12974 IN denotes at
T5873 12975-12979 NN denotes 37ºC
T5874 12981-12984 DT denotes The
T5875 12985-12989 NN denotes dose
T5876 12990-12992 IN denotes of
T5877 12993-12996 NN denotes H89
T5878 12997-13000 VB denotes was
T5879 13001-13007 VB denotes chosen
T5880 13008-13013 VB denotes based
T5881 13014-13016 IN denotes on
T5882 13017-13025 JJ denotes previous
T5883 13026-13030 NN denotes work
T5884 13031-13036 WDT denotes which
T5885 13037-13049 VB denotes demonstrated
T5886 13050-13058 JJ denotes specific
T5887 13059-13062 CC denotes and
T5888 13063-13071 JJ denotes complete
T5889 13072-13082 NN denotes inhibition
T5890 13083-13085 IN denotes of
T5891 13086-13089 NN denotes PKA
T5892 13090-13092 IN denotes at
T5893 13093-13097 DT denotes this
T5894 13098-13114 NN denotes concentration.10
T5895 13115-13120 IN denotes Since
T5896 13121-13124 DT denotes the
T5897 13125-13133 NN denotes activity
T5898 13134-13136 IN denotes of
T5899 13137-13141 DT denotes this
T5900 13142-13151 NN denotes inhibitor
T5901 13152-13154 VB denotes is
T5902 13155-13159 CC denotes both
T5903 13160-13168 JJ denotes specific
T5904 13169-13172 CC denotes and
T5905 13173-13183 JJ denotes consistent
T5906 13184-13186 IN denotes at
T5907 13187-13191 DT denotes this
T5908 13192-13201 VB denotes specified
T5909 13202-13206 NN denotes dose
T5910 13206-13207 -COMMA- denotes ,
T5911 13208-13210 PRP denotes we
T5912 13211-13214 VB denotes did
T5913 13215-13218 RB denotes not
T5914 13219-13226 VB denotes include
T5915 13227-13238 NN denotes experiments
T5916 13239-13243 IN denotes with
T5917 13244-13254 JJ denotes additional
T5918 13255-13258 NN denotes PKA
T5919 13259-13269 NN denotes inhibitors
T5920 13270-13274 IN denotes with
T5921 13275-13279 DT denotes this
T5922 13280-13283 NN denotes set
T5923 13284-13286 IN denotes of
T5924 13287-13298 NN denotes experiments
T5925 13300-13303 DT denotes The
T5926 13304-13309 NN denotes TNF-α
T5927 13310-13323 NN denotes concentration
T5928 13324-13326 IN denotes in
T5929 13327-13330 DT denotes the
T5930 13331-13342 NN denotes supernatant
T5931 13343-13346 VB denotes was
T5932 13347-13355 VB denotes measured
T5933 13356-13370 RB denotes quantitatively
T5934 13371-13373 IN denotes by
T5935 13374-13379 NN denotes ELISA
T5936 13381-13384 DT denotes The
T5937 13385-13392 NN denotes results
T5938 13393-13396 VB denotes are
T5939 13397-13406 VB denotes expressed
T5940 13407-13409 IN denotes in
T5941 13410-13415 NN denotes pg/mL
T6140 13418-13429 JJ denotes Statistical
T6141 13430-13438 NN denotes Analysis
T6142 13439-13442 DT denotes The
T6143 13443-13455 JJ denotes experimental
T6144 13456-13463 NN denotes results
T6145 13464-13472 VB denotes obtained
T6146 13473-13475 IN denotes in
T6147 13476-13480 DT denotes this
T6148 13481-13486 NN denotes study
T6149 13487-13491 VB denotes were
T6150 13492-13499 VB denotes derived
T6151 13500-13504 IN denotes from
T6152 13505-13509 CD denotes four
T6153 13510-13518 JJ denotes separate
T6154 13519-13530 NN denotes experiments
T6155 13531-13535 IN denotes with
T6156 13536-13543 JJ denotes healthy
T6157 13544-13553 NN denotes volunteer
T6158 13554-13560 NN denotes donors
T6159 13562-13566 DT denotes Each
T6160 13567-13572 NN denotes assay
T6161 13573-13576 VB denotes was
T6162 13577-13586 VB denotes performed
T6163 13587-13589 IN denotes in
T6164 13590-13599 NN denotes duplicate
T6165 13600-13602 CC denotes or
T6166 13603-13613 VB denotes triplicate
T6167 13614-13619 WRB denotes where
T6168 13620-13631 JJ denotes appropriate
T6169 13633-13637 NN denotes Data
T6170 13638-13640 VB denotes is
T6171 13641-13650 VB denotes presented
T6172 13651-13653 IN denotes as
T6173 13654-13657 DT denotes the
T6174 13658-13662 JJ denotes mean
T6175 13663-13664 NN denotes ±
T6176 13665-13668 NN denotes SEM
T6177 13670-13681 JJ denotes Statistical
T6178 13682-13693 NN denotes differences
T6179 13694-13701 IN denotes between
T6180 13702-13708 NN denotes groups
T6181 13709-13713 VB denotes were
T6182 13714-13724 VB denotes determined
T6183 13725-13727 IN denotes by
T6184 13728-13735 JJ denotes one-way
T6185 13736-13744 NN denotes analysis
T6186 13745-13747 IN denotes of
T6187 13748-13756 NN denotes variance
T6188 13757-13758 -LRB- denotes (
T6189 13758-13763 NN denotes ANOVA
T6190 13763-13764 -RRB- denotes )
T6191 13765-13769 IN denotes with
T6192 13770-13771 DT denotes a
T6193 13772-13782 NNP denotes Bonferroni
T6194 13783-13793 NN denotes correction
T6195 13795-13806 JJ denotes Statistical
T6196 13807-13819 NN denotes significance
T6197 13820-13823 VB denotes was
T6198 13824-13831 VB denotes defined
T6199 13832-13834 IN denotes as
T6200 13835-13836 NN denotes P
T6201 13837-13838 SYM denotes <
T6202 13839-13843 CD denotes 0.05
T6293 13855-13858 DT denotes The
T6294 13859-13866 NN denotes effects
T6295 13867-13869 IN denotes of
T6296 13870-13873 NN denotes PTX
T6297 13874-13876 IN denotes on
T6298 13877-13888 JJ denotes LPS-induced
T6299 13889-13894 NN denotes TNF-α
T6300 13895-13905 NN denotes production
T6301 13906-13908 TO denotes To
T6302 13909-13918 VB denotes determine
T6303 13919-13922 DT denotes the
T6304 13923-13931 NN denotes duration
T6305 13932-13934 IN denotes of
T6306 13935-13938 NN denotes PDE
T6307 13939-13949 NN denotes inhibition
T6308 13950-13952 IN denotes on
T6309 13953-13958 NN denotes TNF-α
T6310 13959-13969 NN denotes production
T6311 13970-13975 IN denotes after
T6312 13976-13979 NN denotes LPS
T6313 13980-13991 NN denotes stimulation
T6314 13991-13992 -COMMA- denotes ,
T6315 13993-13997 NN denotes time
T6316 13998-14004 NN denotes course
T6317 14005-14012 NN denotes studies
T6318 14013-14017 VB denotes were
T6319 14018-14027 VB denotes conducted
T6320 14029-14031 IN denotes In
T6321 14032-14042 NN denotes accordance
T6322 14043-14047 IN denotes with
T6323 14048-14053 JJ denotes prior
T6324 14054-14058 NN denotes work
T6325 14058-14059 -COMMA- denotes ,
T6326 14060-14063 NN denotes PTX
T6327 14064-14067 VB denotes had
T6328 14068-14069 DT denotes a
T6329 14070-14075 JJ denotes rapid
T6330 14076-14079 CC denotes and
T6331 14080-14089 JJ denotes sustained
T6332 14090-14096 NN denotes effect
T6333 14097-14099 IN denotes on
T6334 14100-14105 NN denotes TNF-α
T6335 14106-14116 NN denotes production
T6336 14117-14119 FW denotes in
T6337 14120-14125 FW denotes vitro
T6338 14126-14127 -LRB- denotes (
T6339 14127-14133 NN denotes Figure
T6340 14134-14135 CD denotes 1
T6341 14135-14136 -RRB- denotes )
T6696 14139-14142 NN denotes PTX
T6697 14143-14152 VB denotes decreases
T6698 14153-14164 JJ denotes LPS-induced
T6699 14165-14170 NN denotes NF-κB
T6700 14171-14181 NN denotes activation
T6701 14182-14187 IN denotes Since
T6702 14188-14203 NN denotes phosphorylation
T6703 14204-14209 VB denotes leads
T6704 14210-14212 TO denotes to
T6705 14213-14227 NN denotes ubiquitination
T6706 14228-14231 CC denotes and
T6707 14232-14243 NN denotes degradation
T6708 14244-14246 IN denotes of
T6709 14247-14252 JJ denotes I-κBα
T6710 14253-14256 CC denotes and
T6711 14257-14267 JJ denotes subsequent
T6712 14268-14275 JJ denotes nuclear
T6713 14276-14289 NN denotes translocation
T6714 14290-14292 IN denotes of
T6715 14293-14298 NN denotes NF-κB
T6716 14298-14299 -COMMA- denotes ,
T6717 14300-14302 PRP denotes we
T6718 14303-14308 RB denotes first
T6719 14309-14317 VB denotes examined
T6720 14318-14321 DT denotes the
T6721 14322-14329 NN denotes effects
T6722 14330-14332 IN denotes of
T6723 14333-14336 NN denotes PTX
T6724 14337-14339 IN denotes on
T6725 14340-14345 NN denotes I-κBα
T6726 14347-14358 JJ denotes Cytoplasmic
T6727 14359-14364 JJ denotes I-κBα
T6728 14365-14380 NN denotes phosphorylation
T6729 14381-14384 VB denotes was
T6730 14385-14393 RB denotes markedly
T6731 14394-14403 VB denotes increased
T6732 14404-14413 VB denotes following
T6733 14414-14417 NN denotes LPS
T6734 14418-14429 NN denotes stimulation
T6735 14430-14434 WRB denotes when
T6736 14435-14443 VB denotes compared
T6737 14444-14446 TO denotes to
T6738 14447-14454 NN denotes control
T6739 14455-14456 -LRB- denotes (
T6740 14456-14459 CD denotes 100
T6741 14460-14461 SYM denotes ±
T6742 14462-14463 CD denotes 0
T6743 14464-14467 IN denotes vs.
T6744 14468-14470 CD denotes 20
T6745 14471-14472 CC denotes ±
T6746 14473-14475 CD denotes 18
T6747 14475-14476 -COLON- denotes ;
T6748 14477-14478 NN denotes P
T6749 14479-14480 SYM denotes <
T6750 14481-14485 CD denotes 0.05
T6751 14485-14486 -RRB- denotes )
T6752 14488-14491 DT denotes The
T6753 14492-14500 NN denotes addition
T6754 14501-14503 IN denotes of
T6755 14504-14507 NN denotes PTX
T6756 14508-14521 RB denotes significantly
T6757 14522-14535 VB denotes downregulated
T6758 14536-14547 JJ denotes LPS-induced
T6759 14548-14553 NN denotes I-κBα
T6760 14554-14569 NN denotes phosphorylation
T6761 14570-14571 -LRB- denotes (
T6762 14571-14572 NN denotes P
T6763 14573-14574 SYM denotes =
T6764 14575-14579 CD denotes 0.02
T6765 14579-14580 -COLON- denotes ;
T6766 14581-14587 NN denotes Figure
T6767 14588-14589 CD denotes 2
T6768 14589-14590 -RRB- denotes )
T6769 14591-14593 IN denotes In
T6770 14594-14595 DT denotes a
T6771 14596-14603 JJ denotes similar
T6772 14604-14611 NN denotes fashion
T6773 14611-14612 -COMMA- denotes ,
T6774 14613-14620 JJ denotes nuclear
T6775 14621-14626 NN denotes NF-κB
T6776 14627-14630 NN denotes p65
T6777 14631-14646 NN denotes phosphorylation
T6778 14647-14650 VB denotes was
T6779 14651-14660 VB denotes increased
T6780 14661-14670 VB denotes following
T6781 14671-14674 NN denotes LPS
T6782 14675-14686 NN denotes stimulation
T6783 14687-14688 -LRB- denotes (
T6784 14688-14691 CD denotes 100
T6785 14692-14693 SYM denotes ±
T6786 14694-14695 CD denotes 0
T6787 14696-14699 IN denotes vs.
T6788 14700-14702 CD denotes 38
T6789 14703-14704 CC denotes ±
T6790 14705-14707 CD denotes 20
T6791 14707-14708 -COLON- denotes ;
T6792 14709-14710 NN denotes P
T6793 14711-14712 SYM denotes <
T6794 14713-14717 CD denotes 0.01
T6795 14717-14718 -RRB- denotes )
T6796 14720-14723 NN denotes PTX
T6797 14724-14733 RB denotes similarly
T6798 14734-14743 VB denotes decreased
T6799 14744-14755 JJ denotes LPS-induced
T6800 14756-14761 NN denotes NF-κB
T6801 14762-14765 NN denotes p65
T6802 14766-14773 JJ denotes nuclear
T6803 14774-14789 NN denotes phosphorylation
T6804 14789-14790 -COMMA- denotes ,
T6805 14791-14792 DT denotes a
T6806 14793-14799 NN denotes marker
T6807 14800-14802 IN denotes of
T6808 14803-14808 NN denotes NF-κB
T6809 14809-14819 NN denotes activation
T6810 14820-14823 CC denotes and
T6811 14824-14831 JJ denotes nuclear
T6812 14832-14845 NN denotes translocation
T6813 14846-14847 -LRB- denotes (
T6814 14847-14850 CD denotes 100
T6815 14851-14852 SYM denotes ±
T6816 14853-14854 CD denotes 0
T6817 14855-14858 IN denotes vs.
T6818 14859-14861 CD denotes 40
T6819 14862-14863 CC denotes ±
T6820 14864-14865 CD denotes 6
T6821 14865-14866 -COLON- denotes ;
T6822 14867-14868 NN denotes P
T6823 14869-14874 CD denotes =0.03
T6824 14874-14875 -COLON- denotes ;
T6825 14876-14882 NN denotes Figure
T6826 14883-14885 NN denotes 3A
T6827 14885-14886 -RRB- denotes )
T6828 14888-14893 NN denotes EMSAs
T6829 14894-14898 VB denotes were
T6830 14899-14903 RB denotes then
T6831 14904-14913 VB denotes performed
T6832 14914-14916 TO denotes to
T6833 14917-14923 VB denotes verify
T6834 14924-14927 DT denotes the
T6835 14928-14939 JJ denotes PTX-induced
T6836 14940-14951 NN denotes alterations
T6837 14952-14954 IN denotes in
T6838 14955-14962 JJ denotes nuclear
T6839 14963-14978 NN denotes phosphorylation
T6840 14979-14987 VB denotes resulted
T6841 14988-14990 IN denotes in
T6842 14991-14998 JJ denotes similar
T6843 14999-15006 NN denotes effects
T6844 15007-15009 IN denotes on
T6845 15010-15013 DT denotes the
T6846 15014-15017 NN denotes DNA
T6847 15018-15025 NN denotes binding
T6848 15026-15034 NN denotes activity
T6849 15035-15037 IN denotes of
T6850 15038-15043 NN denotes NF-κB
T6851 15045-15048 DT denotes The
T6852 15049-15057 NN denotes addition
T6853 15058-15060 IN denotes of
T6854 15061-15064 NN denotes PTX
T6855 15065-15067 TO denotes to
T6856 15068-15082 JJ denotes LPS-stimulated
T6857 15083-15094 JJ denotes mononuclear
T6858 15095-15100 NN denotes cells
T6859 15101-15109 VB denotes resulted
T6860 15110-15112 IN denotes in
T6861 15113-15123 JJ denotes comparable
T6862 15124-15138 NN denotes downregulation
T6863 15139-15141 IN denotes of
T6864 15142-15145 NN denotes DNA
T6865 15146-15153 NN denotes binding
T6866 15154-15162 NN denotes activity
T6867 15162-15163 -COMMA- denotes ,
T6868 15164-15171 JJ denotes similar
T6869 15172-15174 TO denotes to
T6870 15175-15178 DT denotes the
T6871 15179-15187 VB denotes observed
T6872 15188-15202 NN denotes downregulation
T6873 15203-15205 IN denotes of
T6874 15206-15211 NN denotes NF-κB
T6875 15212-15227 NN denotes phosphorylation
T6876 15228-15229 -LRB- denotes (
T6877 15229-15235 NN denotes Figure
T6878 15236-15238 NN denotes 3B
T6879 15238-15239 -RRB- denotes )
T7258 15242-15245 NN denotes PTX
T7259 15246-15257 VB denotes upregulates
T7260 15258-15262 NN denotes CREB
T7261 15263-15278 NN denotes phosphorylation
T7262 15279-15282 CC denotes and
T7263 15283-15293 NN denotes activation
T7264 15294-15299 IN denotes after
T7265 15300-15303 NN denotes LPS
T7266 15304-15315 NN denotes stimulation
T7267 15316-15331 NN denotes Phosphorylation
T7268 15332-15334 IN denotes of
T7269 15335-15342 JJ denotes nuclear
T7270 15343-15347 NN denotes CREB
T7271 15348-15351 VB denotes was
T7272 15352-15356 VB denotes used
T7273 15357-15359 IN denotes as
T7274 15360-15361 DT denotes a
T7275 15362-15368 NN denotes marker
T7276 15369-15372 IN denotes for
T7277 15373-15377 NN denotes CREB
T7278 15378-15388 NN denotes activation
T7279 15390-15393 NN denotes LPS
T7280 15394-15405 NN denotes stimulation
T7281 15406-15412 VB denotes caused
T7282 15413-15414 DT denotes a
T7283 15415-15425 JJ denotes negligible
T7284 15426-15434 NN denotes increase
T7285 15435-15437 IN denotes in
T7286 15438-15442 NN denotes CREB
T7287 15443-15458 NN denotes phosphorylation
T7288 15459-15463 WRB denotes when
T7289 15464-15472 VB denotes compared
T7290 15473-15475 TO denotes to
T7291 15476-15483 NN denotes control
T7292 15484-15485 -LRB- denotes (
T7293 15485-15491 NN denotes Figure
T7294 15492-15494 NN denotes 4A
T7295 15494-15495 -RRB- denotes )
T7296 15497-15500 NN denotes PTX
T7297 15501-15506 RB denotes alone
T7298 15507-15513 VB denotes caused
T7299 15514-15515 DT denotes a
T7300 15516-15522 JJ denotes marked
T7301 15523-15531 NN denotes increase
T7302 15532-15534 IN denotes in
T7303 15535-15539 NN denotes CREB
T7304 15540-15555 NN denotes phosphorylation
T7305 15556-15557 -LRB- denotes (
T7306 15557-15558 NN denotes P
T7307 15559-15560 JJ denotes <
T7308 15561-15565 CD denotes 0.01
T7309 15566-15569 CC denotes vs.
T7310 15570-15574 NN denotes HBSS
T7311 15574-15575 -RRB- denotes )
T7312 15577-15581 WRB denotes When
T7313 15582-15585 NN denotes LPS
T7314 15586-15589 CC denotes and
T7315 15590-15593 NN denotes PTX
T7316 15594-15602 NN denotes exposure
T7317 15603-15611 VB denotes occurred
T7318 15612-15626 RB denotes simultaneously
T7319 15626-15627 -COMMA- denotes ,
T7320 15628-15632 NN denotes CREB
T7321 15633-15648 NN denotes phosphorylation
T7322 15649-15652 VB denotes was
T7323 15653-15666 RB denotes significantly
T7324 15667-15673 JJ denotes higher
T7325 15674-15678 IN denotes than
T7326 15679-15683 IN denotes with
T7327 15684-15687 NN denotes LPS
T7328 15688-15699 NN denotes stimulation
T7329 15700-15705 RB denotes alone
T7330 15706-15707 -LRB- denotes (
T7331 15707-15710 CD denotes 543
T7332 15711-15712 CC denotes ±
T7333 15713-15715 CD denotes 92
T7334 15716-15719 IN denotes vs.
T7335 15720-15723 CD denotes 100
T7336 15724-15725 SYM denotes ±
T7337 15726-15727 CD denotes 0
T7338 15727-15728 -COLON- denotes ;
T7339 15729-15730 NN denotes P
T7340 15731-15732 SYM denotes <
T7341 15733-15737 CD denotes 0.01
T7342 15737-15738 -RRB- denotes )
T7343 15740-15742 IN denotes In
T7344 15743-15751 NN denotes addition
T7345 15751-15752 -COMMA- denotes ,
T7346 15753-15756 DT denotes the
T7347 15757-15763 NN denotes amount
T7348 15764-15766 IN denotes of
T7349 15767-15771 NN denotes CREB
T7350 15772-15787 NN denotes phosphorylation
T7351 15788-15792 VB denotes seen
T7352 15793-15797 IN denotes with
T7353 15798-15809 JJ denotes concomitant
T7354 15810-15813 NN denotes LPS
T7355 15814-15817 CC denotes and
T7356 15818-15821 NN denotes PTX
T7357 15822-15831 NN denotes treatment
T7358 15832-15835 VB denotes was
T7359 15836-15840 JJ denotes less
T7360 15841-15845 IN denotes than
T7361 15846-15850 DT denotes that
T7362 15851-15855 VB denotes seen
T7363 15856-15860 IN denotes with
T7364 15861-15864 NN denotes PTX
T7365 15865-15870 RB denotes alone
T7366 15870-15871 -COMMA- denotes ,
T7367 15872-15880 IN denotes although
T7368 15881-15885 DT denotes this
T7369 15886-15896 NN denotes difference
T7370 15897-15900 VB denotes was
T7371 15901-15904 RB denotes not
T7372 15905-15918 RB denotes statistically
T7373 15919-15930 JJ denotes significant
T7374 15931-15932 -LRB- denotes (
T7375 15932-15933 NN denotes P
T7376 15934-15935 SYM denotes =
T7377 15936-15940 CD denotes 0.08
T7378 15940-15941 -RRB- denotes )
T7379 15943-15945 TO denotes To
T7380 15946-15955 VB denotes determine
T7381 15956-15958 IN denotes if
T7382 15959-15967 NN denotes CREB-DNA
T7383 15968-15975 NN denotes binding
T7384 15976-15979 VB denotes was
T7385 15980-15988 VB denotes affected
T7386 15989-15991 IN denotes by
T7387 15992-15995 NN denotes PTX
T7388 15996-15998 IN denotes in
T7389 15999-16000 DT denotes a
T7390 16001-16007 NN denotes manner
T7391 16008-16015 JJ denotes similar
T7392 16016-16018 TO denotes to
T7393 16019-16023 NN denotes CREB
T7394 16024-16039 NN denotes phosphorylation
T7395 16039-16040 -COMMA- denotes ,
T7396 16041-16043 DT denotes an
T7397 16044-16048 NN denotes EMSA
T7398 16049-16052 VB denotes was
T7399 16053-16062 VB denotes performed
T7400 16064-16072 NN denotes Exposure
T7401 16073-16075 IN denotes of
T7402 16076-16090 JJ denotes LPS-stimulated
T7403 16091-16096 NN denotes cells
T7404 16097-16099 TO denotes to
T7405 16100-16103 NN denotes PTX
T7406 16104-16113 RB denotes similarly
T7407 16114-16123 VB denotes increased
T7408 16124-16128 NN denotes CREB
T7409 16129-16140 NN denotes DNA-binding
T7410 16141-16149 VB denotes compared
T7411 16150-16152 TO denotes to
T7412 16153-16156 NN denotes LPS
T7413 16157-16162 RB denotes alone
T7414 16163-16164 -LRB- denotes (
T7415 16164-16170 NN denotes Figure
T7416 16171-16173 NN denotes 4B
T7417 16173-16174 -RRB- denotes )
T7755 16177-16183 NN denotes Effect
T7756 16184-16186 IN denotes of
T7757 16187-16190 NN denotes PTX
T7758 16191-16193 IN denotes on
T7759 16194-16199 NN denotes TNF-α
T7760 16200-16210 NN denotes production
T7761 16211-16213 IN denotes in
T7762 16214-16217 DT denotes the
T7763 16218-16226 NN denotes presence
T7764 16227-16229 IN denotes of
T7765 16230-16233 NN denotes PKA
T7766 16234-16244 NN denotes inhibition
T7767 16245-16248 DT denotes The
T7768 16249-16253 NN denotes role
T7769 16254-16256 IN denotes of
T7770 16257-16260 NN denotes PKA
T7771 16261-16263 IN denotes in
T7772 16264-16275 JJ denotes PTX-induced
T7773 16276-16290 NN denotes downregulation
T7774 16291-16293 IN denotes of
T7775 16294-16299 NN denotes TNF-α
T7776 16300-16310 NN denotes production
T7777 16311-16313 IN denotes in
T7778 16314-16328 JJ denotes LPS-stimulated
T7779 16329-16340 JJ denotes mononuclear
T7780 16341-16346 NN denotes cells
T7781 16347-16350 VB denotes was
T7782 16351-16359 VB denotes assessed
T7783 16360-16362 IN denotes by
T7784 16363-16371 VB denotes treating
T7785 16372-16383 JJ denotes mononuclear
T7786 16384-16389 NN denotes cells
T7787 16390-16394 IN denotes with
T7788 16395-16398 NN denotes H89
T7789 16398-16399 -COMMA- denotes ,
T7790 16400-16401 DT denotes a
T7791 16402-16405 NN denotes PKA
T7792 16406-16415 NN denotes inhibitor
T7793 16415-16416 -COMMA- denotes ,
T7794 16417-16422 JJ denotes prior
T7795 16423-16425 TO denotes to
T7796 16426-16429 NN denotes LPS
T7797 16430-16433 CC denotes and
T7798 16434-16437 NN denotes PTX
T7799 16438-16446 NN denotes exposure
T7800 16448-16451 DT denotes The
T7801 16452-16460 NN denotes addition
T7802 16461-16464 NN denotes PTX
T7803 16465-16467 TO denotes to
T7804 16468-16482 JJ denotes LPS-stimulated
T7805 16483-16488 NN denotes cells
T7806 16489-16497 VB denotes resulted
T7807 16498-16500 IN denotes in
T7808 16501-16502 DT denotes a
T7809 16503-16512 NN denotes reduction
T7810 16513-16515 IN denotes in
T7811 16516-16521 NN denotes TNF-α
T7812 16521-16522 -COMMA- denotes ,
T7813 16523-16530 JJ denotes similar
T7814 16531-16533 TO denotes to
T7815 16534-16538 DT denotes that
T7816 16539-16547 VB denotes observed
T7817 16548-16550 IN denotes in
T7818 16551-16554 PRP-DOLLAR- denotes our
T7819 16555-16563 JJ denotes previous
T7820 16564-16575 NN denotes experiments
T7821 16576-16577 -LRB- denotes (
T7822 16577-16580 CD denotes 529
T7823 16581-16586 NN denotes pg/mL
T7824 16587-16588 IN denotes ±
T7825 16589-16592 CD denotes 112
T7826 16593-16596 IN denotes vs.
T7827 16597-16599 CD denotes 37
T7828 16600-16605 NN denotes pg/mL
T7829 16606-16607 NN denotes ±
T7830 16608-16610 CD denotes 10
T7831 16610-16611 -COLON- denotes ;
T7832 16612-16613 NN denotes P
T7833 16614-16615 SYM denotes <
T7834 16616-16620 CD denotes 0.01
T7835 16620-16621 -RRB- denotes )
T7836 16623-16637 NN denotes Pre-incubation
T7837 16638-16640 IN denotes of
T7838 16641-16646 NN denotes cells
T7839 16647-16651 IN denotes with
T7840 16652-16655 NN denotes H89
T7841 16656-16661 JJ denotes prior
T7842 16662-16664 TO denotes to
T7843 16665-16668 NN denotes LPS
T7844 16669-16672 CC denotes and
T7845 16673-16676 NN denotes PTX
T7846 16677-16685 NN denotes exposure
T7847 16686-16689 VB denotes did
T7848 16690-16693 RB denotes not
T7849 16694-16700 VB denotes result
T7850 16701-16703 IN denotes in
T7851 16704-16707 DT denotes any
T7852 16708-16719 JJ denotes significant
T7853 16720-16730 NN denotes modulation
T7854 16731-16733 IN denotes of
T7855 16734-16739 NN denotes TNF-α
T7856 16740-16750 NN denotes production
T7857 16751-16755 WRB denotes when
T7858 16756-16764 VB denotes compared
T7859 16765-16767 TO denotes to
T7860 16768-16772 DT denotes that
T7861 16773-16785 VB denotes demonstrated
T7862 16786-16790 IN denotes with
T7863 16791-16794 NN denotes PTX
T7864 16795-16800 RB denotes alone
T7865 16801-16802 -LRB- denotes (
T7866 16802-16803 NN denotes P
T7867 16804-16805 SYM denotes =
T7868 16806-16809 CD denotes 0.2
T7869 16809-16810 -COLON- denotes ;
T7870 16811-16817 NN denotes Figure
T7871 16818-16819 CD denotes 5
T7872 16819-16820 -RRB- denotes )
T7873 16822-16827 DT denotes These
T7874 16828-16835 NN denotes results
T7875 16836-16843 VB denotes suggest
T7876 16844-16848 IN denotes that
T7877 16849-16863 NN denotes downregulation
T7878 16864-16866 IN denotes of
T7879 16867-16872 NN denotes TNF-α
T7880 16873-16883 NN denotes production
T7881 16884-16886 IN denotes by
T7882 16887-16890 NN denotes PTX
T7883 16891-16893 IN denotes in
T7884 16894-16908 JJ denotes LPS-stimulated
T7885 16909-16920 JJ denotes mononuclear
T7886 16921-16926 NN denotes cells
T7887 16927-16929 VB denotes is
T7888 16929-16930 -COMMA- denotes ,
T7889 16931-16933 IN denotes at
T7890 16934-16939 JJ denotes least
T7891 16940-16942 IN denotes in
T7892 16943-16947 NN denotes part
T7893 16947-16948 -COMMA- denotes ,
T7894 16949-16964 JJ denotes PKA-independent
T4026 8519-8521 IN denotes on
T4048 8668-8670 TO denotes to
R349 T451 T452 arg1Of Insights,into
R350 T451 T462 arg1Of Insights,:
R351 T454 T452 arg2Of Regulation,into
R352 T454 T453 arg1Of Regulation,the
R353 T454 T455 arg1Of Regulation,of
R354 T454 T458 arg1Of Regulation,in
R355 T457 T455 arg2Of Production,of
R356 T457 T456 arg1Of Production,TNF-α
R357 T461 T458 arg2Of Cells,in
R358 T461 T459 arg1Of Cells,Human
R359 T461 T460 arg1Of Cells,Mononuclear
R360 T464 T463 arg1Of Effects,The
R361 T464 T465 arg1Of Effects,of
R362 T468 T465 arg2Of Inhibition,of
R363 T468 T466 arg1Of Inhibition,Non-Specific
R364 T468 T467 arg1Of Inhibition,Phosphodiesterase
R365 T471 T470 arg1Of objective,The
R366 T471 T472 arg1Of objective,of
R367 T471 T475 arg1Of objective,was
R368 T474 T472 arg2Of study,of
R369 T474 T473 arg1Of study,this
R370 T477 T475 arg2Of determine,was
R371 T477 T476 arg1Of determine,to
R372 T479 T477 arg2Of effect,determine
R373 T479 T478 arg1Of effect,the
R374 T479 T480 arg1Of effect,of
R375 T479 T484 arg1Of effect,on
R376 T483 T480 arg2Of inhibition,of
R377 T483 T481 arg1Of inhibition,nonspecific
R378 T483 T482 arg1Of inhibition,phosphodiesterase
R379 T487 T485 arg1Of activation,transcription
R380 T487 T486 arg1Of activation,factor
R381 T487 T488 arg1Of activation,and
R382 T488 T484 arg2Of and,on
R383 T488 T496 arg1Of and,in
R384 T491 T489 arg1Of factor-alpha,tumor
R385 T491 T490 arg1Of factor-alpha,necrosis
R386 T491 T492 arg1Of factor-alpha,(
R387 T493 T492 arg2Of TNF-α,(
R388 T494 T492 arg3Of ),(
R389 T495 T488 arg2Of production,and
R390 T495 T491 arg1Of production,factor-alpha
R391 T499 T498 arg2Of LPS,(
R392 T500 T498 arg3Of ),(
R393 T501 T497 arg1Of -stimulated,lipopolysaccharide
R394 T501 T498 arg1Of -stimulated,(
R395 T504 T496 arg2Of cells,in
R396 T504 T501 arg1Of cells,-stimulated
R397 T504 T502 arg1Of cells,human
R398 T504 T503 arg1Of cells,mononuclear
R399 T507 T506 arg1Of production,The
R400 T507 T508 arg1Of production,of
R401 T507 T510 arg1Of production,following
R402 T507 T513 arg1Of production,is
R403 T509 T508 arg2Of TNF-α,of
R404 T512 T510 arg2Of stimulation,following
R405 T512 T511 arg1Of stimulation,LPS
R406 T514 T513 arg2Of one,is
R407 T514 T515 arg1Of one,of
R408 T518 T515 arg2Of steps,of
R409 T518 T516 arg1Of steps,the
R410 T518 T517 arg1Of steps,key
R411 T518 T519 arg1Of steps,in
R412 T521 T522 arg1Of sepsis,and
R413 T522 T519 arg2Of and,in
R414 T522 T520 arg1Of and,bacterial
R415 T523 T522 arg2Of inflammation,and
R416 T525 T524 arg1Of mechanism,The
R417 T525 T526 arg2Of mechanism,by
R418 T525 T527 arg1Of mechanism,which
R419 T525 T538 arg1Of mechanism,remains
R420 T525 T539 arg1Of mechanism,unclear
R421 T529 T528 arg1Of inhibition,phosphodiesterase
R422 T529 T530 arg1Of inhibition,alters
R423 T530 T526 arg1Of alters,by
R424 T530 T533 arg1Of alters,in
R425 T532 T530 arg2Of production,alters
R426 T532 T531 arg1Of production,TNF-α
R427 T535 T533 arg2Of presence,in
R428 T535 T534 arg1Of presence,the
R429 T535 T536 arg1Of presence,of
R430 T537 T536 arg2Of LPS,of
R431 T539 T538 arg2Of unclear,remains
R432 T543 T541 arg1Of cells,Human
R433 T543 T542 arg1Of cells,mononuclear
R434 T543 T544 arg1Of cells,were
R435 T543 T545 arg2Of cells,stimulated
R436 T545 T544 arg2Of stimulated,were
R437 T545 T546 arg1Of stimulated,with
R438 T547 T546 arg2Of LPS,with
R439 T547 T548 arg1Of LPS,(
R440 T547 T552 arg1Of LPS,","
R441 T547 T553 arg1Of LPS,in
R442 T547 T566 arg1Of LPS,","
R443 T550 T548 arg2Of μg/mL,(
R444 T550 T549 arg1Of μg/mL,1
R445 T551 T548 arg3Of ),(
R446 T555 T556 arg1Of presence,and
R447 T556 T553 arg2Of and,in
R448 T556 T554 arg1Of and,the
R449 T556 T558 arg1Of and,of
R450 T557 T556 arg2Of absence,and
R451 T559 T558 arg2Of Pentoxifylline,of
R452 T559 T560 arg1Of Pentoxifylline,(
R453 T561 T562 arg1Of PTX,;
R454 T562 T560 arg2Of ;,(
R455 T564 T562 arg2Of mM,;
R456 T564 T563 arg1Of mM,20
R457 T565 T560 arg3Of ),(
R458 T570 T566 arg2Of inhibitor,","
R459 T570 T567 arg1Of inhibitor,a
R460 T570 T568 arg1Of inhibitor,nonspecific
R461 T570 T569 arg1Of inhibitor,phosphodiesterase
R462 T572 T571 arg1Of blotting,Western
R463 T572 T573 arg1Of blotting,of
R464 T572 T594 arg1Of blotting,was
R465 T572 T595 arg2Of blotting,performed
R466 T576 T574 arg2Of I-κBα,phosphorylated
R467 T576 T575 arg1Of I-κBα,cytoplasmic
R468 T576 T577 arg1Of I-κBα,","
R469 T577 T585 arg1Of ",",and
R470 T580 T577 arg2Of p65,","
R471 T580 T578 arg1Of p65,nuclear
R472 T580 T579 arg1Of p65,factor-κB
R473 T580 T581 arg1Of p65,(
R474 T582 T581 arg2Of NF-κB,(
R475 T583 T581 arg3Of ),(
R476 T585 T573 arg2Of and,of
R477 T585 T584 arg1Of and,","
R478 T590 T585 arg2Of protein,and
R479 T590 T586 arg1Of protein,nuclear
R480 T590 T587 arg1Of protein,cAMP-response
R481 T590 T588 arg1Of protein,element
R482 T590 T589 arg1Of protein,binding
R483 T590 T591 arg1Of protein,(
R484 T592 T591 arg2Of CREB,(
R485 T593 T591 arg3Of ),(
R486 T595 T594 arg2Of performed,was
R487 T597 T596 arg1Of binding,DNA
R488 T597 T598 arg1Of binding,of
R489 T597 T602 arg1Of binding,was
R490 T597 T603 arg2Of binding,verified
R491 T599 T600 arg1Of NF-κB,and
R492 T600 T598 arg2Of and,of
R493 T601 T600 arg2Of CREB,and
R494 T603 T602 arg2Of verified,was
R495 T608 T603 arg1Of assay,verified
R496 T608 T604 arg2Of assay,by
R497 T608 T605 arg1Of assay,electrophoretic
R498 T608 T606 arg1Of assay,mobility
R499 T608 T607 arg1Of assay,shift
R500 T610 T609 arg1Of levels,TNF-α
R501 T610 T611 arg1Of levels,were
R502 T610 T612 arg2Of levels,determined
R503 T612 T611 arg2Of determined,were
R504 T612 T613 arg1Of determined,in
R505 T612 T619 arg1Of determined,in
R506 T615 T613 arg2Of supernatant,in
R507 T615 T614 arg1Of supernatant,the
R508 T615 T616 arg1Of supernatant,of
R509 T618 T616 arg2Of cells,of
R510 T618 T617 arg2Of cells,stimulated
R511 T621 T622 arg1Of presence,and
R512 T623 T622 arg2Of absence,and
R513 T627 T619 arg2Of inhibition,in
R514 T627 T620 arg1Of inhibition,the
R515 T627 T621 arg1Of inhibition,presence
R516 T627 T623 arg1Of inhibition,absence
R517 T627 T624 arg1Of inhibition,Protein
R518 T627 T625 arg1Of inhibition,kinase
R519 T627 T626 arg1Of inhibition,A
R520 T627 T628 arg1Of inhibition,by
R521 T632 T628 arg2Of assay,by
R522 T632 T629 arg1Of assay,an
R523 T632 T630 arg1Of assay,enzyme-linked
R524 T632 T631 arg1Of assay,immunosorbent
R525 T632 T633 arg1Of assay,(
R526 T634 T633 arg2Of ELISA,(
R527 T635 T633 arg3Of ),(
R528 T637 T638 arg1Of PTX,was
R529 T637 T639 arg2Of PTX,demonstrated
R530 T637 T642 arg1Of PTX,reduce
R531 T639 T638 arg2Of demonstrated,was
R532 T642 T639 arg3Of reduce,demonstrated
R533 T642 T640 arg1Of reduce,to
R534 T642 T641 arg1Of reduce,significantly
R535 T645 T643 arg1Of phosphorylation,cytoplasmic
R536 T645 T644 arg1Of phosphorylation,I-κBα
R537 T645 T646 arg1Of phosphorylation,","
R538 T646 T651 arg1Of ",",and
R539 T649 T646 arg2Of phosphorylation,","
R540 T649 T647 arg1Of phosphorylation,nuclear
R541 T649 T648 arg1Of phosphorylation,p65
R542 T651 T642 arg2Of and,reduce
R543 T651 T650 arg1Of and,","
R544 T655 T651 arg2Of activity,and
R545 T655 T652 arg1Of activity,the
R546 T655 T653 arg1Of activity,DNA
R547 T655 T654 arg1Of activity,binding
R548 T655 T656 arg1Of activity,of
R549 T657 T656 arg2Of NF-κB,of
R550 T659 T658 arg2Of contrast,In
R551 T661 T663 arg1Of PTX,enhanced
R552 T663 T658 arg1Of enhanced,In
R553 T663 T660 arg1Of enhanced,","
R554 T663 T662 arg1Of enhanced,markedly
R555 T665 T666 arg1Of phosphorylation,and
R556 T666 T663 arg2Of and,enhanced
R557 T666 T664 arg1Of and,the
R558 T666 T670 arg1Of and,of
R559 T669 T666 arg2Of activity,and
R560 T669 T667 arg1Of activity,DNA
R561 T669 T668 arg1Of activity,binding
R562 T671 T670 arg2Of CREB,of
R563 T672 T674 arg2Of Cells,treated
R564 T672 T679 arg1Of Cells,secreted
R565 T674 T673 arg1Of treated,concomitantly
R566 T674 T675 arg1Of treated,with
R567 T676 T677 arg1Of PTX,and
R568 T677 T675 arg2Of and,with
R569 T678 T677 arg2Of LPS,and
R570 T681 T679 arg2Of levels,secreted
R571 T681 T680 arg1Of levels,similar
R572 T681 T682 arg1Of levels,of
R573 T681 T684 arg1Of levels,in
R574 T683 T682 arg2Of TNF-α,of
R575 T686 T687 arg1Of presence,and
R576 T688 T687 arg2Of absence,and
R577 T692 T684 arg2Of inhibition,in
R578 T692 T685 arg1Of inhibition,the
R579 T692 T686 arg1Of inhibition,presence
R580 T692 T688 arg1Of inhibition,absence
R581 T692 T689 arg1Of inhibition,Protein
R582 T692 T690 arg1Of inhibition,kinase
R583 T692 T691 arg1Of inhibition,A
R584 T696 T694 arg1Of level,The
R585 T696 T695 arg2Of level,increased
R586 T696 T697 arg1Of level,of
R587 T696 T699 arg1Of level,that
R588 T696 T700 arg1Of level,results
R589 T696 T704 arg1Of level,affects
R590 T698 T697 arg2Of cAMP,of
R591 T700 T701 arg1Of results,from
R592 T703 T701 arg2Of inhibition,from
R593 T703 T702 arg1Of inhibition,phosphodiesterase
R594 T704 T709 arg1Of affects,","
R595 T704 T710 modOf affects,resulting
R596 T705 T706 arg1Of cytoplasmic,and
R597 T707 T706 arg2Of nuclear,and
R598 T708 T704 arg2Of events,affects
R599 T708 T705 arg1Of events,cytoplasmic
R600 T708 T707 arg1Of events,nuclear
R601 T710 T711 arg1Of resulting,in
R602 T710 T724 arg1Of resulting,through
R603 T713 T712 arg1Of attenuation,the
R604 T713 T714 arg1Of attenuation,of
R605 T713 T716 arg1Of attenuation,and
R606 T715 T714 arg2Of NF-κB,of
R607 T716 T711 arg2Of and,in
R608 T718 T716 arg2Of activation,and
R609 T718 T717 arg1Of activation,the
R610 T718 T719 arg1Of activation,of
R611 T723 T719 arg2Of binding,of
R612 T723 T720 arg1Of binding,CREB
R613 T723 T721 arg1Of binding,transcriptional
R614 T723 T722 arg1Of binding,DNA
R615 T725 T724 arg2Of pathways,through
R616 T725 T726 arg1Of pathways,that
R617 T725 T727 arg1Of pathways,are
R618 T725 T731 arg1Of pathways,A-independent
R619 T727 T728 arg1Of are,partially
R620 T731 T727 arg2Of A-independent,are
R621 T731 T729 arg1Of A-independent,Protein
R622 T731 T730 arg1Of A-independent,kinase
R623 T735 T733 arg1Of inhibition,PTX-mediated
R624 T735 T734 arg1Of inhibition,phosphodiesterase
R625 T735 T736 arg1Of inhibition,occurs
R626 T735 T745 arg1Of inhibition,may
R627 T735 T746 arg1Of inhibition,serve
R628 T736 T737 arg1Of occurs,partially
R629 T736 T738 arg1Of occurs,through
R630 T736 T744 arg1Of occurs,and
R631 T743 T738 arg2Of pathway,through
R632 T743 T739 arg1Of pathway,a
R633 T743 T740 arg1Of pathway,Protein
R634 T743 T741 arg1Of pathway,kinase
R635 T743 T742 arg1Of pathway,A-independent
R636 T746 T744 arg2Of serve,and
R637 T746 T745 arg2Of serve,may
R638 T746 T747 arg1Of serve,as
R639 T750 T747 arg2Of tool,as
R640 T750 T748 arg1Of tool,a
R641 T750 T749 arg1Of tool,useful
R642 T750 T751 arg1Of tool,in
R643 T753 T751 arg2Of attenuation,in
R644 T753 T752 arg1Of attenuation,the
R645 T753 T754 arg1Of attenuation,of
R646 T756 T754 arg2Of inflammation,of
R647 T756 T755 arg1Of inflammation,LPS-induced
R1298 T1616 T1612 arg1Of lipopolysaccharide,The
R1299 T1616 T1613 arg1Of lipopolysaccharide,bacterial
R1300 T1616 T1614 arg1Of lipopolysaccharide,membrane
R1301 T1616 T1615 arg1Of lipopolysaccharide,component
R1302 T1616 T1617 arg1Of lipopolysaccharide,(
R1303 T1616 T1620 arg1Of lipopolysaccharide,is
R1304 T1616 T1621 arg1Of lipopolysaccharide,capable
R1305 T1616 T1623 arg1Of lipopolysaccharide,initiating
R1306 T1618 T1617 arg2Of LPS,(
R1307 T1619 T1617 arg3Of ),(
R1308 T1621 T1620 arg2Of capable,is
R1309 T1621 T1622 arg1Of capable,of
R1310 T1623 T1622 arg2Of initiating,of
R1311 T1624 T1625 arg1Of phosphorylation,and
R1312 T1625 T1623 arg2Of and,initiating
R1313 T1625 T1627 arg1Of and,of
R1314 T1626 T1625 arg2Of activation,and
R1315 T1632 T1628 arg1Of kinases,multiple
R1316 T1632 T1629 arg1Of kinases,host
R1317 T1632 T1630 arg1Of kinases,intracellular
R1318 T1632 T1631 arg1Of kinases,protein
R1319 T1632 T1633 arg1Of kinases,and
R1320 T1633 T1627 arg2Of and,of
R1321 T1635 T1633 arg2Of factors,and
R1322 T1635 T1634 arg1Of factors,transcription
R1323 T1638 T1636 arg1Of activation,Transcription
R1324 T1638 T1637 arg1Of activation,factor
R1325 T1638 T1639 arg1Of activation,","
R1326 T1638 T1640 arg1Of activation,which
R1327 T1638 T1641 arg1Of activation,results
R1328 T1638 T1652 arg1Of activation,is
R1329 T1641 T1642 arg1Of results,in
R1330 T1644 T1642 arg2Of modulation,in
R1331 T1644 T1643 arg1Of modulation,the
R1332 T1644 T1645 arg1Of modulation,of
R1333 T1647 T1646 arg1Of transcription,gene
R1334 T1647 T1648 arg1Of transcription,and
R1335 T1648 T1645 arg2Of and,of
R1336 T1650 T1648 arg2Of synthesis,and
R1337 T1650 T1649 arg1Of synthesis,protein
R1338 T1652 T1651 arg1Of is,","
R1339 T1655 T1652 arg2Of element,is
R1340 T1655 T1653 arg1Of element,a
R1341 T1655 T1654 arg1Of element,critical
R1342 T1655 T1656 arg1Of element,in
R1343 T1659 T1656 arg2Of mechanism,in
R1344 T1659 T1657 arg1Of mechanism,the
R1345 T1659 T1658 arg1Of mechanism,defense
R1346 T1659 T1660 arg1Of mechanism,of
R1347 T1664 T1660 arg2Of "system.1,2",of
R1348 T1664 T1661 arg1Of "system.1,2",the
R1349 T1664 T1662 arg1Of "system.1,2",host
R1350 T1664 T1663 arg1Of "system.1,2",immune
R1351 T1668 T1665 arg1Of activation,LPS-induced
R1352 T1668 T1666 arg1Of activation,transcription
R1353 T1668 T1667 arg1Of activation,factor
R1354 T1668 T1669 arg1Of activation,has
R1355 T1668 T1670 arg1Of activation,been
R1356 T1668 T1671 arg2Of activation,shown
R1357 T1668 T1673 arg1Of activation,be
R1358 T1671 T1669 arg2Of shown,has
R1359 T1671 T1670 arg2Of shown,been
R1360 T1673 T1671 arg3Of be,shown
R1361 T1673 T1672 arg1Of be,to
R1362 T1676 T1673 arg2Of regulator,be
R1363 T1676 T1674 arg1Of regulator,a
R1364 T1676 T1675 arg1Of regulator,key
R1365 T1676 T1677 arg1Of regulator,of
R1366 T1676 T1686 modOf regulator,is
R1367 T1680 T1677 arg2Of factor-α,of
R1368 T1680 T1678 arg1Of factor-α,tumor
R1369 T1680 T1679 arg1Of factor-α,necrosis
R1370 T1682 T1681 arg2Of TNF-α,(
R1371 T1683 T1681 arg3Of ),(
R1372 T1685 T1681 arg1Of TNF-α,(
R1373 T1685 T1684 arg1Of TNF-α,production.3
R1374 T1685 T1686 arg1Of TNF-α,is
R1375 T1690 T1686 arg2Of cytokine,is
R1376 T1690 T1687 arg1Of cytokine,a
R1377 T1690 T1688 arg1Of cytokine,potent
R1378 T1690 T1689 arg1Of cytokine,pro-inflammatory
R1379 T1690 T1691 arg2Of cytokine,involved
R1380 T1691 T1692 arg1Of involved,in
R1381 T1695 T1692 arg2Of spectrum,in
R1382 T1695 T1693 arg1Of spectrum,a
R1383 T1695 T1694 arg1Of spectrum,wide
R1384 T1695 T1696 arg1Of spectrum,of
R1385 T1698 T1696 arg2Of responses,of
R1386 T1698 T1697 arg1Of responses,cellular
R1387 T1702 T1701 arg1Of synthesis,TNF-α
R1388 T1702 T1703 arg1Of synthesis,can
R1389 T1702 T1704 arg1Of synthesis,be
R1390 T1702 T1705 arg2Of synthesis,attenuated
R1391 T1705 T1703 arg2Of attenuated,can
R1392 T1705 T1704 arg2Of attenuated,be
R1393 T1705 T1706 arg1Of attenuated,in
R1394 T1705 T1716 arg1Of attenuated,after
R1395 T1708 T1706 arg2Of cells,in
R1396 T1708 T1707 arg1Of cells,immune
R1397 T1708 T1709 arg2Of cells,exposed
R1398 T1709 T1710 arg1Of exposed,to
R1399 T1711 T1712 arg1Of phosphodiesterase,(
R1400 T1713 T1712 arg2Of PDE,(
R1401 T1714 T1712 arg3Of ),(
R1402 T1715 T1710 arg2Of inhibition,to
R1403 T1715 T1711 arg1Of inhibition,phosphodiesterase
R1404 T1717 T1716 arg2Of challenge,after
R1405 T1717 T1718 arg1Of challenge,by
R1406 T1720 T1718 arg2Of variety,by
R1407 T1720 T1719 arg1Of variety,a
R1408 T1720 T1721 arg1Of variety,of
R1409 T1723 T1721 arg2Of stimulants.4,of
R1410 T1723 T1722 arg1Of stimulants.4,pro-inflammatory
R1411 T1726 T1724 arg1Of mechanisms,The
R1412 T1726 T1725 arg1Of mechanisms,signaling
R1413 T1726 T1727 arg2Of mechanisms,affected
R1414 T1726 T1731 arg1Of mechanisms,","
R1415 T1726 T1732 arg1Of mechanisms,which
R1416 T1726 T1734 arg1Of mechanisms,lead
R1417 T1726 T1742 arg1Of mechanisms,have
R1418 T1726 T1744 arg1Of mechanisms,been
R1419 T1726 T1746 arg2Of mechanisms,characterized
R1420 T1730 T1727 arg1Of inhibition,affected
R1421 T1730 T1728 arg2Of inhibition,by
R1422 T1730 T1729 arg1Of inhibition,PDE
R1423 T1734 T1733 arg1Of lead,ultimately
R1424 T1734 T1735 arg1Of lead,to
R1425 T1737 T1735 arg2Of downregulation,to
R1426 T1737 T1736 arg1Of downregulation,the
R1427 T1737 T1738 arg1Of downregulation,of
R1428 T1740 T1738 arg2Of production,of
R1429 T1740 T1739 arg1Of production,TNF-α
R1430 T1746 T1699 arg1Of characterized,Furthermore
R1431 T1746 T1700 arg1Of characterized,","
R1432 T1746 T1703 modOf characterized,can
R1433 T1746 T1741 arg1Of characterized,","
R1434 T1746 T1742 arg2Of characterized,have
R1435 T1746 T1743 arg1Of characterized,not
R1436 T1746 T1744 arg2Of characterized,been
R1437 T1746 T1745 arg1Of characterized,well
R1438 T1746 T1747 arg1Of characterized,in
R1439 T1749 T1747 arg2Of cells,in
R1440 T1749 T1748 arg1Of cells,inflammatory
R1441 T1752 T1753 arg1Of it,has
R1442 T1752 T1754 arg1Of it,been
R1443 T1752 T1755 arg2Of it,demonstrated
R1444 T1755 T1750 arg1Of demonstrated,Classically
R1445 T1755 T1751 arg1Of demonstrated,","
R1446 T1755 T1753 arg2Of demonstrated,has
R1447 T1755 T1754 arg2Of demonstrated,been
R1448 T1758 T1757 arg1Of inhibition,PDE
R1449 T1758 T1759 arg1Of inhibition,results
R1450 T1759 T1760 arg1Of results,in
R1451 T1759 T1773 arg1Of results,and
R1452 T1763 T1760 arg2Of accumulation,in
R1453 T1763 T1761 arg1Of accumulation,the
R1454 T1763 T1762 arg1Of accumulation,intracellular
R1455 T1763 T1764 arg1Of accumulation,of
R1456 T1769 T1764 arg2Of "adenosine-3,5-monophosphate",of
R1457 T1769 T1765 arg1Of "adenosine-3,5-monophosphate",the
R1458 T1769 T1766 arg1Of "adenosine-3,5-monophosphate",second
R1459 T1769 T1767 arg1Of "adenosine-3,5-monophosphate",messenger
R1460 T1769 T1768 arg1Of "adenosine-3,5-monophosphate",cyclic
R1461 T1769 T1770 arg1Of "adenosine-3,5-monophosphate",(
R1462 T1771 T1770 arg2Of cAMP,(
R1463 T1772 T1770 arg3Of ),(
R1464 T1773 T1810 arg1Of and,and
R1465 T1775 T1774 arg1Of activation,subsequent
R1466 T1775 T1776 arg1Of activation,of
R1467 T1775 T1787 arg1Of activation,leads
R1468 T1781 T1780 arg2Of PKA,(
R1469 T1782 T1780 arg3Of ),(
R1470 T1785 T1776 arg2Of activation,of
R1471 T1785 T1777 arg1Of activation,Protein
R1472 T1785 T1778 arg1Of activation,kinase
R1473 T1785 T1779 arg1Of activation,A
R1474 T1785 T1780 arg1Of activation,(
R1475 T1785 T1783 arg1Of activation,.5
R1476 T1785 T1784 arg1Of activation,PKA
R1477 T1787 T1773 arg2Of leads,and
R1478 T1787 T1786 arg1Of leads,then
R1479 T1787 T1788 arg1Of leads,to
R1480 T1790 T1788 arg2Of phosphorylation,to
R1481 T1790 T1789 arg1Of phosphorylation,the
R1482 T1790 T1791 arg1Of phosphorylation,of
R1483 T1798 T1793 arg1Of protein,transcription
R1484 T1798 T1794 arg1Of protein,factor
R1485 T1798 T1795 arg1Of protein,cAMP-response
R1486 T1798 T1796 arg1Of protein,element
R1487 T1798 T1797 arg1Of protein,binding
R1488 T1798 T1799 arg1Of protein,(
R1489 T1798 T1802 arg1Of protein,","
R1490 T1800 T1799 arg2Of CREB,(
R1491 T1801 T1799 arg3Of ),(
R1492 T1802 T1791 arg2Of ",",of
R1493 T1802 T1792 arg1Of ",",the
R1494 T1803 T1802 arg2Of transmission,","
R1495 T1803 T1804 arg1Of transmission,of
R1496 T1803 T1806 arg1Of transmission,into
R1497 T1805 T1804 arg2Of signals,of
R1498 T1808 T1806 arg2Of nucleus,into
R1499 T1808 T1807 arg1Of nucleus,the
R1500 T1810 T1755 arg3Of and,demonstrated
R1501 T1810 T1756 arg1Of and,that
R1502 T1810 T1809 arg1Of and,","
R1503 T1813 T1811 arg1Of modulation,the
R1504 T1813 T1812 arg1Of modulation,subsequent
R1505 T1813 T1814 arg1Of modulation,of
R1506 T1813 T1822 arg1Of modulation,does
R1507 T1813 T1825 arg1Of modulation,explain
R1508 T1816 T1815 arg1Of transcription.6,gene
R1509 T1819 T1818 arg1Of simple,apparently
R1510 T1821 T1814 arg2Of cascade,of
R1511 T1821 T1816 arg1Of cascade,transcription.6
R1512 T1821 T1817 arg1Of cascade,This
R1513 T1821 T1819 arg1Of cascade,simple
R1514 T1821 T1820 arg1Of cascade,linear
R1515 T1825 T1810 arg2Of explain,and
R1516 T1825 T1822 arg2Of explain,does
R1517 T1825 T1823 arg1Of explain,not
R1518 T1825 T1824 arg1Of explain,fully
R1519 T1827 T1825 arg2Of mechanism,explain
R1520 T1827 T1826 arg1Of mechanism,the
R1521 T1827 T1828 arg2Of mechanism,by
R1522 T1827 T1829 arg1Of mechanism,which
R1523 T1831 T1830 arg1Of elevation,an
R1524 T1831 T1832 arg1Of elevation,in
R1525 T1831 T1837 arg1Of elevation,exerts
R1526 T1836 T1832 arg2Of level,in
R1527 T1836 T1833 arg1Of level,the
R1528 T1836 T1834 arg1Of level,intracellular
R1529 T1836 T1835 arg1Of level,cAMP
R1530 T1837 T1828 arg1Of exerts,by
R1531 T1837 T1840 arg1Of exerts,on
R1532 T1839 T1837 arg2Of effects,exerts
R1533 T1839 T1838 arg1Of effects,wide-ranging
R1534 T1843 T1840 arg2Of functions,on
R1535 T1843 T1841 arg1Of functions,multiple
R1536 T1843 T1842 arg1Of functions,cellular
R1537 T1844 T1845 arg1Of There,is
R1538 T1848 T1845 arg2Of body,is
R1539 T1848 T1846 arg1Of body,a
R1540 T1848 T1847 arg1Of body,growing
R1541 T1848 T1849 arg1Of body,of
R1542 T1848 T1851 arg1Of body,suggesting
R1543 T1850 T1849 arg2Of evidence,of
R1544 T1853 T1854 arg1Of cAMP,may
R1545 T1853 T1855 arg1Of cAMP,function
R1546 T1855 T1851 arg2Of function,suggesting
R1547 T1855 T1852 arg1Of function,that
R1548 T1855 T1854 arg2Of function,may
R1549 T1855 T1856 arg1Of function,through
R1550 T1858 T1859 arg1Of PKA-dependent,and
R1551 T1860 T1859 arg2Of -independent,and
R1552 T1861 T1856 arg2Of mechanisms.6–8,through
R1553 T1861 T1857 arg1Of mechanisms.6–8,both
R1554 T1861 T1858 arg1Of mechanisms.6–8,PKA-dependent
R1555 T1861 T1860 arg1Of mechanisms.6–8,-independent
R1556 T1863 T1862 arg1Of activation,LPS-induced
R1557 T1863 T1864 arg1Of activation,of
R1558 T1863 T1873 arg1Of activation,has
R1559 T1863 T1875 arg1Of activation,been
R1560 T1869 T1864 arg2Of factor-κB,of
R1561 T1869 T1865 arg1Of factor-κB,the
R1562 T1869 T1866 arg1Of factor-κB,transcription
R1563 T1869 T1867 arg1Of factor-κB,factor
R1564 T1869 T1868 arg1Of factor-κB,nuclear
R1565 T1869 T1870 arg1Of factor-κB,(
R1566 T1871 T1870 arg2Of NF-κB,(
R1567 T1872 T1870 arg3Of ),(
R1568 T1875 T1873 arg2Of been,has
R1569 T1875 T1874 arg1Of been,also
R1570 T1877 T1875 arg2Of focus,been
R1571 T1877 T1876 arg1Of focus,the
R1572 T1877 T1878 arg1Of focus,of
R1573 T1881 T1878 arg2Of deal,of
R1574 T1881 T1879 arg1Of deal,a
R1575 T1881 T1880 arg1Of deal,great
R1576 T1881 T1882 arg1Of deal,of
R1577 T1883 T1882 arg2Of research,of
R1578 T1884 T1885 arg1Of It,has
R1579 T1884 T1886 arg1Of It,been
R1580 T1884 T1888 arg2Of It,demonstrated
R1581 T1888 T1885 arg2Of demonstrated,has
R1582 T1888 T1886 arg2Of demonstrated,been
R1583 T1888 T1887 arg1Of demonstrated,clearly
R1584 T1890 T1891 arg1Of agents,that
R1585 T1890 T1892 arg1Of agents,increase
R1586 T1890 T1896 arg1Of agents,inhibit
R1587 T1894 T1892 arg2Of cAMP,increase
R1588 T1894 T1893 arg1Of cAMP,intracellular
R1589 T1896 T1888 arg3Of inhibit,demonstrated
R1590 T1896 T1889 arg1Of inhibit,that
R1591 T1896 T1895 arg1Of inhibit,also
R1592 T1900 T1896 arg2Of transcription,inhibit
R1593 T1900 T1897 arg1Of transcription,NF-κB-dependent
R1594 T1900 T1898 arg1Of transcription,pro-inflammatory
R1595 T1900 T1899 arg1Of transcription,gene
R1596 T1900 T1901 arg1Of transcription,","
R1597 T1900 T1903 arg1Of transcription,of
R1598 T1903 T1902 arg1Of of,particularly
R1599 T1906 T1903 arg2Of gene.9,of
R1600 T1906 T1904 arg1Of gene.9,the
R1601 T1906 T1905 arg1Of gene.9,TNF-α
R1602 T1907 T1908 arg1Of Controversy,exists
R1603 T1908 T1909 arg1Of exists,regarding
R1604 T1912 T1909 arg2Of mechanism,regarding
R1605 T1912 T1910 arg1Of mechanism,the
R1606 T1912 T1911 arg1Of mechanism,exact
R1607 T1912 T1913 arg1Of mechanism,(
R1608 T1912 T1916 arg2Of mechanism,by
R1609 T1912 T1917 arg1Of mechanism,which
R1610 T1914 T1913 arg2Of s,(
R1611 T1915 T1913 arg3Of ),(
R1612 T1919 T1918 arg1Of inhibition,PDE
R1613 T1919 T1920 arg1Of inhibition,down-regulates
R1614 T1920 T1916 arg1Of down-regulates,by
R1615 T1922 T1920 arg2Of production,down-regulates
R1616 T1922 T1921 arg1Of production,TNF-α
R1617 T1923 T1924 arg1Of Possibilities,include
R1618 T1923 T1927 arg1Of Possibilities,are
R1619 T1923 T1929 arg2Of Possibilities,limited
R1620 T1924 T1926 arg1Of include,but
R1621 T1926 T1925 arg1Of but,","
R1622 T1926 T1931 arg1Of but,","
R1623 T1929 T1926 arg2Of limited,but
R1624 T1929 T1927 arg2Of limited,are
R1625 T1929 T1928 arg1Of limited,not
R1626 T1929 T1930 arg1Of limited,to
R1627 T1932 T1933 arg1Of inhibition,of
R1628 T1932 T1945 arg1Of inhibition,increased
R1629 T1937 T1934 arg1Of activity,NF-κB
R1630 T1937 T1935 arg1Of activity,DNA
R1631 T1937 T1936 arg1Of activity,binding
R1632 T1937 T1938 arg1Of activity,","
R1633 T1938 T1933 arg2Of ",",of
R1634 T1939 T1938 arg2Of downregulation,","
R1635 T1939 T1940 arg1Of downregulation,of
R1636 T1943 T1940 arg2Of activity,of
R1637 T1943 T1941 arg1Of activity,NF-κB
R1638 T1943 T1942 arg1Of activity,transcriptional
R1639 T1945 T1931 arg2Of increased,","
R1640 T1945 T1944 arg1Of increased,","
R1641 T1947 T1946 arg1Of activation,CREB
R1642 T1947 T1949 arg1Of activation,and
R1643 T1949 T1945 arg2Of and,increased
R1644 T1949 T1948 arg1Of and,","
R1645 T1950 T1949 arg2Of competition,and
R1646 T1950 T1951 arg1Of competition,between
R1647 T1952 T1953 arg1Of NF-κB,and
R1648 T1953 T1951 arg2Of and,between
R1649 T1953 T1955 arg1Of and,for
R1650 T1954 T1953 arg2Of CREB,and
R1651 T1957 T1955 arg2Of co-activators,for
R1652 T1957 T1956 arg1Of co-activators,common
R1653 T1957 T1959 arg1Of co-activators,as
R1654 T1959 T1958 arg1Of as,such
R1655 T1962 T1959 arg2Of protein,as
R1656 T1962 T1960 arg1Of protein,CREB
R1657 T1962 T1961 arg1Of protein,binding
R1658 T1962 T1963 arg1Of protein,(
R1659 T1964 T1963 arg2Of CBP,(
R1660 T1965 T1963 arg3Of ),(
R1661 T1966 T1967 arg1Of It,is
R1662 T1966 T1970 arg2Of It,known
R1663 T1970 T1967 arg2Of known,is
R1664 T1970 T1968 arg1Of known,also
R1665 T1970 T1969 arg1Of known,not
R1666 T1973 T1972 arg1Of processes,these
R1667 T1973 T1974 arg1Of processes,rely
R1668 T1974 T1970 arg3Of rely,known
R1669 T1974 T1971 arg1Of rely,whether
R1670 T1974 T1975 arg1Of rely,solely
R1671 T1974 T1976 arg1Of rely,upon
R1672 T1978 T1976 arg2Of activation,upon
R1673 T1978 T1977 arg1Of activation,the
R1674 T1978 T1979 arg1Of activation,of
R1675 T1980 T1979 arg2Of PKA,of
R1676 T1984 T1983 arg1Of objective,the
R1677 T1984 T1985 arg1Of objective,of
R1678 T1984 T1989 arg1Of objective,is
R1679 T1988 T1985 arg2Of study,of
R1680 T1988 T1986 arg1Of study,the
R1681 T1988 T1987 arg1Of study,present
R1682 T1989 T1981 arg1Of is,Therefore
R1683 T1989 T1982 arg1Of is,","
R1684 T1991 T1989 arg2Of determine,is
R1685 T1991 T1990 arg1Of determine,to
R1686 T1991 T1998 arg1Of determine,with
R1687 T1991 T2015 arg1Of determine,vitro
R1688 T1991 T2016 arg1Of determine,in
R1689 T1993 T1991 arg2Of effects,determine
R1690 T1993 T1992 arg1Of effects,the
R1691 T1993 T1994 arg1Of effects,of
R1692 T1997 T1994 arg2Of inhibition,of
R1693 T1997 T1995 arg1Of inhibition,nonspecific
R1694 T1997 T1996 arg1Of inhibition,PDE
R1695 T2001 T2000 arg2Of 5-oxohexyl,[
R1696 T2002 T2000 arg3Of ],[
R1697 T2003 T1998 arg2Of "-3,7-dimethylxanthine",with
R1698 T2003 T1999 arg1Of "-3,7-dimethylxanthine",1-
R1699 T2003 T2000 arg1Of "-3,7-dimethylxanthine",[
R1700 T2003 T2004 arg1Of "-3,7-dimethylxanthine",(
R1701 T2003 T2009 arg1Of "-3,7-dimethylxanthine",on
R1702 T2005 T2004 arg2Of Pentoxifylline,(
R1703 T2005 T2006 arg1Of Pentoxifylline,;
R1704 T2007 T2006 arg2Of PTX,;
R1705 T2008 T2004 arg3Of ),(
R1706 T2010 T2011 arg1Of NF-κB,and
R1707 T2012 T2011 arg2Of CREB,and
R1708 T2013 T2009 arg2Of activation,on
R1709 T2013 T2010 arg1Of activation,NF-κB
R1710 T2013 T2012 arg1Of activation,CREB
R1711 T2015 T2014 arg1Of vitro,in
R1712 T2019 T2016 arg2Of cells,in
R1713 T2019 T2017 arg1Of cells,human
R1714 T2019 T2018 arg1Of cells,mononuclear
R1715 T2022 T2020 arg2Of use,With
R1716 T2022 T2021 arg1Of use,the
R1717 T2022 T2023 arg1Of use,of
R1718 T2025 T2023 arg2Of inhibitors,of
R1719 T2025 T2024 arg1Of inhibitors,specific
R1720 T2027 T2029 arg1Of we,investigated
R1721 T2029 T2020 arg1Of investigated,With
R1722 T2029 T2026 arg1Of investigated,","
R1723 T2029 T2028 arg1Of investigated,also
R1724 T2031 T2029 arg2Of role,investigated
R1725 T2031 T2030 arg1Of role,the
R1726 T2031 T2032 arg1Of role,of
R1727 T2031 T2034 arg1Of role,in
R1728 T2033 T2032 arg2Of PKA,of
R1729 T2037 T2034 arg2Of production,in
R1730 T2037 T2035 arg1Of production,LPS-induced
R1731 T2037 T2036 arg1Of production,TNF-α
R2218 T2792 T2791 arg1Of study,this
R2219 T2792 T2793 arg1Of study,was
R2220 T2792 T2794 arg2Of study,approved
R2221 T2794 T2793 arg2Of approved,was
R2222 T2794 T2810 arg1Of approved,in
R2223 T2800 T2796 arg1Of Program,the
R2224 T2800 T2797 arg1Of Program,Human
R2225 T2800 T2798 arg1Of Program,Research
R2226 T2800 T2799 arg1Of Program,Protections
R2227 T2800 T2801 arg1Of Program,and
R2228 T2801 T2794 arg1Of and,approved
R2229 T2801 T2795 arg2Of and,by
R2230 T2806 T2803 arg1Of Written,Institutional
R2231 T2806 T2804 arg1Of Written,Review
R2232 T2806 T2805 arg1Of Written,Board.
R2233 T2807 T2801 arg2Of consent,and
R2234 T2807 T2802 arg1Of consent,the
R2235 T2807 T2806 arg1Of consent,Written
R2236 T2807 T2808 modOf consent,to
R2237 T2809 T2808 arg1Of participate,to
R2238 T2812 T2811 arg1Of study,the
R2239 T2812 T2813 arg1Of study,was
R2240 T2812 T2814 arg2Of study,obtained
R2241 T2814 T2813 arg2Of obtained,was
R2242 T2814 T2815 arg1Of obtained,from
R2243 T2814 T2818 arg1Of obtained,prior
R2244 T2817 T2815 arg2Of volunteers,from
R2245 T2817 T2816 arg1Of volunteers,all
R2246 T2818 T2819 arg1Of prior,to
R2247 T2820 T2819 arg2Of blood,to
R2248 T2822 T2821 arg1Of Vacutainers,"donation. Heparinized"
R2249 T2822 T2823 arg1Of Vacutainers,were
R2250 T2822 T2824 arg2Of Vacutainers,purchased
R2251 T2824 T2810 arg2Of purchased,in
R2252 T2824 T2813 modOf purchased,was
R2253 T2824 T2823 arg2Of purchased,were
R2254 T2824 T2825 arg1Of purchased,from
R2255 T2827 T2825 arg2Of Dickinson,from
R2256 T2827 T2826 arg1Of Dickinson,Becton
R2257 T2827 T2828 arg1Of Dickinson,(
R2258 T2830 T2828 arg2Of Jose,(
R2259 T2830 T2829 arg1Of Jose,San
R2260 T2830 T2831 arg1Of Jose,","
R2261 T2832 T2831 arg2Of CA,","
R2262 T2833 T2828 arg3Of ),(
R2263 T2837 T2834 arg1Of tubes,1.5-mL
R2264 T2837 T2835 arg1Of tubes,Eppendorf
R2265 T2837 T2836 arg1Of tubes,centrifuge
R2266 T2837 T2838 arg1Of tubes,were
R2267 T2837 T2839 arg2Of tubes,purchased
R2268 T2839 T2838 arg2Of purchased,were
R2269 T2839 T2840 arg1Of purchased,from
R2270 T2842 T2840 arg2Of Scientific,from
R2271 T2842 T2841 arg1Of Scientific,Fisher
R2272 T2842 T2843 arg1Of Scientific,(
R2273 T2844 T2843 arg2Of Pittsburg,(
R2274 T2844 T2845 arg1Of Pittsburg,","
R2275 T2844 T2846 arg1Of Pittsburg,PA
R2276 T2847 T2843 arg3Of ),(
R2277 T2851 T2848 arg1Of solution,Hank’s
R2278 T2851 T2849 arg1Of solution,balanced
R2279 T2851 T2850 arg1Of solution,salt
R2280 T2851 T2852 arg1Of solution,(
R2281 T2851 T2855 arg1Of solution,and
R2282 T2853 T2852 arg2Of HBSS,(
R2283 T2854 T2852 arg3Of ),(
R2284 T2855 T2858 arg1Of and,were
R2285 T2855 T2859 arg2Of and,obtained
R2286 T2856 T2855 arg2Of RPMI,and
R2287 T2856 T2857 arg1Of RPMI,1640
R2288 T2859 T2858 arg2Of obtained,were
R2289 T2859 T2860 arg1Of obtained,from
R2290 T2862 T2860 arg2Of Scientific,from
R2291 T2862 T2861 arg1Of Scientific,Irvine
R2292 T2862 T2863 arg1Of Scientific,(
R2293 T2865 T2863 arg2Of Ana,(
R2294 T2865 T2864 arg1Of Ana,Santa
R2295 T2865 T2866 arg1Of Ana,","
R2296 T2867 T2866 arg2Of CA,","
R2297 T2868 T2863 arg3Of ),(
R2298 T2871 T2869 arg1Of coli serotype,LPS
R2299 T2871 T2870 arg1Of coli serotype,from Escherichia
R2300 T2871 T2872 arg1Of coli serotype,0111
R2301 T2871 T2873 arg1Of coli serotype,:
R2302 T2871 T2877 arg1Of coli serotype,were
R2303 T2871 T2878 arg2Of coli serotype,purchased
R2304 T2874 T2875 arg1Of B4,and
R2305 T2875 T2873 arg2Of and,:
R2306 T2876 T2875 arg2Of PTX,and
R2307 T2878 T2877 arg2Of purchased,were
R2308 T2878 T2879 arg1Of purchased,from
R2309 T2880 T2879 arg2Of Sigma,from
R2310 T2880 T2881 arg1Of Sigma,(
R2311 T2883 T2881 arg2Of Louis,(
R2312 T2883 T2882 arg1Of Louis,St.
R2313 T2883 T2884 arg1Of Louis,","
R2314 T2885 T2884 arg2Of MO,","
R2315 T2886 T2881 arg3Of ),(
R2316 T2889 T2887 arg1Of inhibitor,The
R2317 T2889 T2888 arg1Of inhibitor,PKA
R2318 T2889 T2890 arg1Of inhibitor,","
R2319 T2891 T2890 arg2Of N-,","
R2320 T2891 T2892 arg1Of N-,[
R2321 T2893 T2892 arg2Of 2-,[
R2322 T2893 T2894 arg1Of 2-,(
R2323 T2893 T2900 arg1Of 2-,ethyl
R2324 T2896 T2895 arg2Of p-bromocinnamyl,(
R2325 T2897 T2895 arg3Of ),(
R2326 T2898 T2894 arg2Of amino,(
R2327 T2898 T2895 arg1Of amino,(
R2328 T2899 T2894 arg3Of ),(
R2329 T2901 T2892 arg3Of ],[
R2330 T2902 T2889 arg1Of -5-isoguinolinesulfonamide-2HCl,inhibitor
R2331 T2902 T2903 arg1Of -5-isoguinolinesulfonamide-2HCl,]
R2332 T2902 T2904 arg1Of -5-isoguinolinesulfonamide-2HCl,(
R2333 T2902 T2908 arg1Of -5-isoguinolinesulfonamide-2HCl,was
R2334 T2902 T2909 arg2Of -5-isoguinolinesulfonamide-2HCl,purchased
R2335 T2905 T2904 arg2Of H89,(
R2336 T2906 T2904 arg3Of ),(
R2337 T2909 T2907 arg1Of purchased,","
R2338 T2909 T2908 arg2Of purchased,was
R2339 T2909 T2910 arg1Of purchased,from
R2340 T2911 T2910 arg2Of Calbiochem,from
R2341 T2911 T2912 arg1Of Calbiochem,(
R2342 T2914 T2912 arg2Of Jolla,(
R2343 T2914 T2913 arg1Of Jolla,La
R2344 T2914 T2915 arg1Of Jolla,","
R2345 T2916 T2915 arg2Of CA,","
R2346 T2917 T2912 arg3Of ),(
R2347 T2918 T2919 arg1Of Antibodies,for
R2348 T2918 T2945 arg1Of Antibodies,and
R2349 T2921 T2920 arg2Of CREB,phosphorylated
R2350 T2921 T2922 arg1Of CREB,(
R2351 T2921 T2926 arg1Of CREB,","
R2352 T2923 T2922 arg2Of serine,(
R2353 T2923 T2924 arg1Of serine,133
R2354 T2925 T2922 arg3Of ),(
R2355 T2926 T2935 arg1Of ",",","
R2356 T2928 T2926 arg2Of I-κBα,","
R2357 T2928 T2927 arg2Of I-κBα,phosphorylated
R2358 T2928 T2929 arg1Of I-κBα,(
R2359 T2931 T2932 arg1Of 32,and
R2360 T2932 T2929 arg2Of and,(
R2361 T2932 T2930 arg1Of and,serines
R2362 T2933 T2932 arg2Of 36,and
R2363 T2934 T2929 arg3Of ),(
R2364 T2935 T2919 arg2Of ",",for
R2365 T2939 T2935 arg2Of subunit,","
R2366 T2939 T2936 arg2Of subunit,phosphorylated
R2367 T2939 T2937 arg1Of subunit,NF-κB
R2368 T2939 T2938 arg1Of subunit,p65
R2369 T2939 T2940 arg1Of subunit,(
R2370 T2941 T2940 arg2Of serine,(
R2371 T2941 T2942 arg1Of serine,276
R2372 T2943 T2940 arg3Of ),(
R2373 T2945 T2944 arg1Of and,","
R2374 T2945 T2948 arg1Of and,were
R2375 T2945 T2949 arg2Of and,purchased
R2376 T2947 T2945 arg2Of antibodies,and
R2377 T2947 T2946 arg1Of antibodies,secondary
R2378 T2949 T2948 arg2Of purchased,were
R2379 T2949 T2950 arg1Of purchased,from
R2380 T2949 T2953 arg1Of purchased,(
R2381 T2952 T2950 arg2Of Signaling,from
R2382 T2952 T2951 arg1Of Signaling,Cell
R2383 T2954 T2953 arg2Of Beverly,(
R2384 T2954 T2955 arg1Of Beverly,","
R2385 T2954 T2956 arg1Of Beverly,MA
R2386 T2957 T2953 arg3Of ),(
R2387 T2959 T2960 arg1Of T500,and
R2388 T2960 T2958 arg1Of and,". Dextran"
R2389 T2960 T2962 arg1Of and,were
R2390 T2960 T2963 arg2Of and,received
R2391 T2961 T2960 arg2Of Percoll,and
R2392 T2963 T2948 modOf received,were
R2393 T2963 T2962 arg2Of received,were
R2394 T2963 T2964 arg1Of received,from
R2395 T2967 T2964 arg2Of Biosciences,from
R2396 T2967 T2965 arg1Of Biosciences,GE
R2397 T2967 T2966 arg1Of Biosciences,Healthcare/Amersham
R2398 T2967 T2968 arg1Of Biosciences,(
R2399 T2969 T2968 arg2Of Piscataway,(
R2400 T2969 T2970 arg1Of Piscataway,","
R2401 T2969 T2971 arg1Of Piscataway,NJ
R2402 T2972 T2968 arg3Of ),(
R2403 T2976 T2973 arg1Of assay,The
R2404 T2976 T2974 arg1Of assay,enzyme-linked
R2405 T2976 T2975 arg1Of assay,immunosorbent
R2406 T2976 T2977 arg1Of assay,(
R2407 T2976 T2980 arg1Of assay,for
R2408 T2976 T2982 arg1Of assay,was
R2409 T2976 T2983 arg2Of assay,obtained
R2410 T2978 T2977 arg2Of ELISA,(
R2411 T2979 T2977 arg3Of ),(
R2412 T2981 T2980 arg2Of TNF-α,for
R2413 T2983 T2982 arg2Of obtained,was
R2414 T2983 T2984 arg1Of obtained,from
R2415 T2983 T3004 arg1Of obtained,and
R2416 T2989 T2985 arg1Of Systems,Quantikine
R2417 T2989 T2986 arg1Of Systems,R
R2418 T2989 T2987 arg1Of Systems,&
R2419 T2989 T2988 arg1Of Systems,D
R2420 T2989 T2990 arg1Of Systems,(
R2421 T2991 T2990 arg2Of Minneapolis,(
R2422 T2991 T2992 arg1Of Minneapolis,","
R2423 T2991 T2993 arg1Of Minneapolis,MN
R2424 T2994 T2990 arg3Of ),(
R2425 T2995 T2989 arg1Of ". Tris-glycine",Systems
R2426 T2995 T2996 arg1Of ". Tris-glycine",and
R2427 T2996 T3000 arg1Of and,","
R2428 T2999 T2996 arg2Of gels,and
R2429 T2999 T2997 arg1Of gels,DNA
R2430 T2999 T2998 arg1Of gels,retardation
R2431 T3000 T2984 arg2Of ",",from
R2432 T3002 T3000 arg2Of membranes,","
R2433 T3002 T3001 arg1Of membranes,nitrocellulose
R2434 T3004 T3003 arg1Of and,","
R2435 T3008 T3005 arg1Of buffers,western
R2436 T3008 T3006 arg1Of buffers,blot
R2437 T3008 T3007 arg1Of buffers,running
R2438 T3008 T3009 arg1Of buffers,were
R2439 T3008 T3010 arg2Of buffers,obtained
R2440 T3010 T3004 arg2Of obtained,and
R2441 T3010 T3009 arg2Of obtained,were
R2442 T3010 T3011 arg1Of obtained,from
R2443 T3010 T3013 arg1Of obtained,(
R2444 T3012 T3011 arg2Of Invitrogen,from
R2445 T3014 T3013 arg2Of Carlsbad,(
R2446 T3014 T3015 arg1Of Carlsbad,","
R2447 T3014 T3016 arg1Of Carlsbad,CA
R2448 T3017 T3013 arg3Of ),(
R2449 T3018 T3019 arg1Of NE-PER® nuclear,and
R2450 T3020 T3019 arg2Of cytoplasmic,and
R2451 T3022 T3018 arg1Of reagents,NE-PER® nuclear
R2452 T3022 T3020 arg1Of reagents,cytoplasmic
R2453 T3022 T3021 arg1Of reagents,extraction
R2454 T3022 T3023 arg1Of reagents,","
R2455 T3023 T3029 arg1Of ",",","
R2456 T3023 T3037 arg1Of ",",and
R2457 T3028 T3023 arg2Of kit,","
R2458 T3028 T3024 arg1Of kit,the
R2459 T3028 T3025 arg1Of kit,BCA
R2460 T3028 T3026 arg1Of kit,protein
R2461 T3028 T3027 arg1Of kit,reagent
R2462 T3035 T3029 arg2Of Kit,","
R2463 T3035 T3030 arg1Of Kit,the
R2464 T3035 T3031 arg1Of Kit,Supersignal
R2465 T3035 T3032 arg1Of Kit,West
R2466 T3035 T3033 arg1Of Kit,Pico
R2467 T3035 T3034 arg1Of Kit,Chemiluminescent
R2468 T3037 T3036 arg1Of and,","
R2469 T3037 T3043 arg1Of and,were
R2470 T3037 T3044 arg2Of and,purchased
R2471 T3041 T3039 arg1Of EMSA,LightShift
R2472 T3041 T3040 arg1Of EMSA,Chemiluminescent
R2473 T3042 T3037 arg2Of Kit,and
R2474 T3042 T3038 arg1Of Kit,the
R2475 T3042 T3041 arg1Of Kit,EMSA
R2476 T3044 T3043 arg2Of purchased,were
R2477 T3044 T3045 arg1Of purchased,from
R2478 T3046 T3045 arg2Of Pierce,from
R2479 T3046 T3047 arg1Of Pierce,(
R2480 T3048 T3047 arg2Of Rockland,(
R2481 T3048 T3049 arg1Of Rockland,","
R2482 T3050 T3049 arg2Of IL,","
R2483 T3051 T3047 arg3Of ),(
R2484 T3052 T3053 arg1Of CREB,and
R2485 T3054 T3053 arg2Of NF-κB,and
R2486 T3057 T3052 arg1Of probes,CREB
R2487 T3057 T3054 arg1Of probes,NF-κB
R2488 T3057 T3055 arg1Of probes,DNA
R2489 T3057 T3056 arg1Of probes,oligonucleotide
R2490 T3057 T3058 arg1Of probes,were
R2491 T3057 T3059 arg2Of probes,synthesized
R2492 T3059 T3058 arg2Of synthesized,were
R2493 T3059 T3062 arg1Of synthesized,(
R2494 T3061 T3059 arg1Of IDT,synthesized
R2495 T3061 T3060 arg2Of IDT,by
R2496 T3063 T3062 arg2Of Coralville,(
R2497 T3063 T3064 arg1Of Coralville,","
R2498 T3063 T3065 arg1Of Coralville,IA
R2499 T3066 T3062 arg3Of ),(
R2500 T3068 T3067 arg1Of membranes,Nylon
R2501 T3068 T3069 arg1Of membranes,were
R2502 T3068 T3070 arg2Of membranes,purchased
R2503 T3070 T3069 arg2Of purchased,were
R2504 T3070 T3071 arg1Of purchased,from
R2505 T3074 T3071 arg2Of Sciences,from
R2506 T3074 T3072 arg1Of Sciences,Roche
R2507 T3074 T3073 arg1Of Sciences,Applied
R2508 T3074 T3075 arg1Of Sciences,(
R2509 T3076 T3075 arg2Of Indianapolis,(
R2510 T3076 T3077 arg1Of Indianapolis,","
R2511 T3076 T3078 arg1Of Indianapolis,IN
R2512 T3079 T3075 arg3Of ),(
R2513 T3083 T3080 arg1Of quantification,Western
R2514 T3083 T3081 arg1Of quantification,blot
R2515 T3083 T3082 arg1Of quantification,band
R2516 T3083 T3084 arg1Of quantification,was
R2517 T3083 T3085 arg2Of quantification,performed
R2518 T3085 T3084 arg2Of performed,was
R2519 T3085 T3086 arg1Of performed,with
R2520 T3090 T3088 arg1Of Digitizing,UN-SCAN-IT
R2521 T3090 T3089 arg1Of Digitizing,Gel
R2522 T3091 T3086 arg2Of software,with
R2523 T3091 T3087 arg1Of software,the
R2524 T3091 T3090 arg1Of software,Digitizing
R2525 T3091 T3092 arg1Of software,(
R2526 T3094 T3093 arg1Of Scientific,Silk
R2527 T3094 T3095 arg1Of Scientific,","
R2528 T3095 T3092 arg2Of ",",(
R2529 T3095 T3097 arg1Of ",",","
R2530 T3096 T3095 arg2Of Orem,","
R2531 T3098 T3097 arg2Of UT,","
R2532 T3099 T3092 arg3Of ),(
R2910 T3581 T3578 arg1Of Cell,Peripheral
R2911 T3581 T3579 arg1Of Cell,Blood
R2912 T3581 T3580 arg1Of Cell,Mononuclear
R2913 T3582 T3583 arg1Of Isolation,and
R2914 T3583 T3577 arg1Of and,Human
R2915 T3583 T3581 arg1Of and,Cell
R2916 T3584 T3583 arg2Of Stimulation,and
R2917 T3587 T3585 arg1Of cells,Human
R2918 T3587 T3586 arg1Of cells,mononuclear
R2919 T3587 T3588 arg1Of cells,were
R2920 T3587 T3589 arg2Of cells,isolated
R2921 T3589 T3588 arg2Of isolated,were
R2922 T3589 T3590 arg1Of isolated,from
R2923 T3593 T3590 arg2Of blood,from
R2924 T3593 T3591 arg1Of blood,the
R2925 T3593 T3592 arg1Of blood,peripheral
R2926 T3593 T3594 arg1Of blood,of
R2927 T3598 T3594 arg2Of volunteers,of
R2928 T3598 T3595 arg1Of volunteers,four
R2929 T3598 T3596 arg1Of volunteers,healthy
R2930 T3598 T3597 arg1Of volunteers,human
R2931 T3601 T3599 arg1Of size,The
R2932 T3601 T3600 arg1Of size,sample
R2933 T3601 T3602 arg1Of size,was
R2934 T3601 T3603 arg2Of size,chosen
R2935 T3603 T3602 arg2Of chosen,was
R2936 T3603 T3604 arg1Of chosen,from
R2937 T3603 T3616 arg1Of chosen,and
R2938 T3607 T3606 arg1Of published,previously
R2939 T3608 T3604 arg2Of work,from
R2940 T3608 T3605 arg1Of work,our
R2941 T3608 T3607 arg1Of work,published
R2942 T3608 T3609 arg1Of work,","
R2943 T3608 T3610 arg1Of work,which
R2944 T3608 T3611 arg1Of work,demonstrated
R2945 T3615 T3611 arg2Of isolation,demonstrated
R2946 T3615 T3612 arg1Of isolation,reliable
R2947 T3615 T3613 arg1Of isolation,results.10
R2948 T3615 T3614 arg1Of isolation,Cell
R2949 T3619 T3617 arg1Of experiments,all
R2950 T3619 T3618 arg1Of experiments,subsequent
R2951 T3619 T3620 arg1Of experiments,were
R2952 T3619 T3621 arg2Of experiments,conducted
R2953 T3621 T3616 arg2Of conducted,and
R2954 T3621 T3620 arg2Of conducted,were
R2955 T3621 T3622 arg1Of conducted,under
R2956 T3623 T3624 arg1Of sterile,and
R2957 T3625 T3624 arg2Of pyrogen-free,and
R2958 T3626 T3622 arg2Of conditions,under
R2959 T3626 T3623 arg1Of conditions,sterile
R2960 T3626 T3625 arg1Of conditions,pyrogen-free
R2961 T3627 T3628 arg2Of Blood,collected
R2962 T3627 T3632 arg1Of Blood,was
R2963 T3627 T3633 arg2Of Blood,incubated
R2964 T3628 T3629 arg1Of collected,in
R2965 T3631 T3629 arg2Of tubes,in
R2966 T3631 T3630 arg1Of tubes,heparin
R2967 T3633 T3632 arg2Of incubated,was
R2968 T3633 T3634 arg1Of incubated,with
R2969 T3633 T3637 arg1Of incubated,and
R2970 T3636 T3634 arg2Of T500,with
R2971 T3636 T3635 arg1Of T500,Dextran
R2972 T3640 T3638 arg1Of cells,the
R2973 T3640 T3639 arg1Of cells,red
R2974 T3640 T3641 arg1Of cells,were
R2975 T3640 T3642 arg2Of cells,sedimented
R2976 T3642 T3637 arg2Of sedimented,and
R2977 T3642 T3641 arg2Of sedimented,were
R2978 T3642 T3643 arg1Of sedimented,for
R2979 T3645 T3643 arg2Of minutes,for
R2980 T3645 T3644 arg1Of minutes,40
R2981 T3645 T3646 arg1Of minutes,at
R2982 T3648 T3646 arg2Of temperature,at
R2983 T3648 T3647 arg1Of temperature,room
R2984 T3651 T3649 arg1Of serum,The
R2985 T3651 T3650 arg1Of serum,resultant
R2986 T3651 T3652 arg1Of serum,was
R2987 T3651 T3654 arg2Of serum,washed
R2988 T3651 T3658 arg2Of serum,separated
R2989 T3654 T3655 arg1Of washed,with
R2990 T3654 T3657 arg1Of washed,and
R2991 T3656 T3655 arg2Of HBSS,with
R2992 T3657 T3652 arg2Of and,was
R2993 T3657 T3653 arg1Of and,then
R2994 T3658 T3657 arg2Of separated,and
R2995 T3658 T3663 arg1Of separated,according
R2996 T3662 T3658 arg1Of centrifugation,separated
R2997 T3662 T3659 arg2Of centrifugation,by
R2998 T3662 T3660 arg1Of centrifugation,Percoll
R2999 T3662 T3661 arg1Of centrifugation,gradient
R3000 T3664 T3663 arg2Of to,according
R3001 T3667 T3664 arg2Of instructions,to
R3002 T3667 T3665 arg1Of instructions,the
R3003 T3667 T3666 arg1Of instructions,manufacturer’s
R3004 T3669 T3668 arg1Of viability,Cell
R3005 T3669 T3670 arg1Of viability,was
R3006 T3669 T3671 arg2Of viability,assessed
R3007 T3671 T3670 arg2Of assessed,was
R3008 T3671 T3677 arg1Of assessed,","
R3009 T3671 T3678 arg1Of assessed,with
R3010 T3676 T3671 arg1Of exclusion,assessed
R3011 T3676 T3672 arg2Of exclusion,by
R3012 T3676 T3673 arg1Of exclusion,trypan
R3013 T3676 T3674 arg1Of exclusion,blue
R3014 T3676 T3675 arg1Of exclusion,dye
R3015 T3679 T3678 arg2Of purity,with
R3016 T3679 T3680 arg1Of purity,of
R3017 T3681 T3682 arg1Of greater,than
R3018 T3683 T3682 arg2Of 95,than
R3019 T3684 T3680 arg2Of %,of
R3020 T3684 T3681 arg1Of %,greater
R3021 T3686 T3685 arg2Of cells,Isolated
R3022 T3686 T3687 arg1Of cells,were
R3023 T3686 T3688 arg2Of cells,resuspended
R3024 T3688 T3687 arg2Of resuspended,were
R3025 T3688 T3689 arg1Of resuspended,in
R3026 T3688 T3701 arg1Of resuspended,at
R3027 T3690 T3689 arg2Of RPMI,in
R3028 T3690 T3691 arg1Of RPMI,1640
R3029 T3690 T3692 arg2Of RPMI,supplemented
R3030 T3692 T3693 arg1Of supplemented,with
R3031 T3696 T3694 arg1Of FBS,10
R3032 T3696 T3695 arg1Of FBS,%
R3033 T3696 T3697 arg1Of FBS,and
R3034 T3697 T3693 arg2Of and,with
R3035 T3698 T3699 arg1Of 5,mM
R3036 T3700 T3697 arg2Of HEPES,and
R3037 T3700 T3698 arg1Of HEPES,5
R3038 T3703 T3701 arg2Of concentration,at
R3039 T3703 T3702 arg1Of concentration,a
R3040 T3703 T3704 arg1Of concentration,of
R3041 T3707 T3704 arg2Of 107cells/mL,of
R3042 T3707 T3705 arg1Of 107cells/mL,1
R3043 T3707 T3706 arg1Of 107cells/mL,x
R3044 T3710 T3708 arg1Of experiment,Each
R3045 T3710 T3709 arg1Of experiment,subsequent
R3046 T3710 T3711 arg2Of experiment,listed
R3047 T3710 T3713 arg1Of experiment,was
R3048 T3710 T3714 arg2Of experiment,conducted
R3049 T3711 T3712 arg1Of listed,below
R3050 T3714 T3713 arg2Of conducted,was
R3051 T3714 T3715 arg1Of conducted,on
R3052 T3716 T3715 arg2Of samples,on
R3053 T3716 T3717 arg1Of samples,of
R3054 T3721 T3717 arg2Of cells,of
R3055 T3721 T3718 arg1Of cells,5
R3056 T3721 T3719 arg1Of cells,x
R3057 T3721 T3720 arg1Of cells,106
R3058 T3721 T3722 arg1Of cells,per
R3059 T3724 T3722 arg2Of group,per
R3060 T3724 T3723 arg1Of group,treatment
R3061 T3726 T3725 arg2Of cells,Isolated
R3062 T3726 T3727 arg1Of cells,were
R3063 T3726 T3728 arg2Of cells,stimulated
R3064 T3728 T3727 arg2Of stimulated,were
R3065 T3728 T3729 arg1Of stimulated,with
R3066 T3728 T3732 arg1Of stimulated,as
R3067 T3731 T3729 arg2Of HBSS,with
R3068 T3731 T3730 arg1Of HBSS,either
R3069 T3735 T3732 arg2Of control,as
R3070 T3735 T3733 arg1Of control,a
R3071 T3735 T3734 arg1Of control,negative
R3072 T3735 T3736 arg1Of control,","
R3073 T3737 T3738 arg1Of LPS,(
R3074 T3737 T3742 arg1Of LPS,","
R3075 T3740 T3738 arg2Of μg/mL,(
R3076 T3740 T3739 arg1Of μg/mL,1
R3077 T3741 T3738 arg3Of ),(
R3078 T3742 T3749 arg1Of ",",or
R3079 T3743 T3742 arg2Of PTX,","
R3080 T3743 T3744 arg1Of PTX,(
R3081 T3746 T3744 arg2Of mM,(
R3082 T3746 T3745 arg1Of mM,20
R3083 T3747 T3744 arg3Of ),(
R3084 T3749 T3736 arg2Of or,","
R3085 T3749 T3748 arg1Of or,","
R3086 T3749 T3754 arg1Of or,","
R3087 T3749 T3755 arg1Of or,at
R3088 T3749 T3759 arg1Of or,","
R3089 T3749 T3760 arg1Of or,for
R3090 T3753 T3749 arg2Of PTX,or
R3091 T3753 T3750 arg1Of PTX,concomitant
R3092 T3753 T3751 arg1Of PTX,LPS
R3093 T3753 T3752 arg1Of PTX,/
R3094 T3758 T3755 arg2Of concentrations,at
R3095 T3758 T3756 arg1Of concentrations,the
R3096 T3758 T3757 arg1Of concentrations,above-mentioned
R3097 T3762 T3760 arg2Of minutes,for
R3098 T3762 T3761 arg1Of minutes,30
R3099 T3762 T3763 arg1Of minutes,at
R3100 T3764 T3763 arg2Of 37ºC,at
R3101 T3765 T3766 arg1Of Cells,were
R3102 T3765 T3767 arg2Of Cells,placed
R3103 T3767 T3766 arg2Of placed,were
R3104 T3767 T3768 arg1Of placed,on
R3105 T3767 T3777 arg1Of placed,and
R3106 T3769 T3768 arg2Of ice,on
R3107 T3769 T3770 arg1Of ice,for
R3108 T3772 T3770 arg2Of minutes,for
R3109 T3772 T3771 arg1Of minutes,10
R3110 T3772 T3774 arg1Of minutes,stop
R3111 T3774 T3770 arg3Of stop,for
R3112 T3774 T3773 arg1Of stop,to
R3113 T3776 T3774 arg2Of reaction,stop
R3114 T3776 T3775 arg1Of reaction,the
R3115 T3779 T3778 arg1Of samples,the
R3116 T3779 T3780 arg1Of samples,were
R3117 T3779 T3782 arg2Of samples,stored
R3118 T3782 T3777 arg2Of stored,and
R3119 T3782 T3780 arg2Of stored,were
R3120 T3782 T3781 arg1Of stored,then
R3121 T3782 T3783 arg1Of stored,at
R3122 T3784 T3783 arg2Of −70°C,at
R3123 T3784 T3785 arg1Of −70°C,for
R3124 T3787 T3785 arg2Of analysis,for
R3125 T3787 T3786 arg1Of analysis,further
R3126 T3790 T3789 arg1Of times,stimulation
R3127 T3790 T3791 arg1Of times,and
R3128 T3791 T3788 arg1Of and,The
R3129 T3791 T3801 arg1Of and,were
R3130 T3791 T3802 arg2Of and,determined
R3131 T3792 T3791 arg2Of concentrations,and
R3132 T3792 T3793 arg1Of concentrations,of
R3133 T3794 T3795 arg1Of LPS,and
R3134 T3795 T3793 arg2Of and,of
R3135 T3795 T3797 arg2Of and,utilized
R3136 T3796 T3795 arg2Of PTX,and
R3137 T3797 T3798 arg1Of utilized,in
R3138 T3800 T3798 arg2Of study,in
R3139 T3800 T3799 arg1Of study,this
R3140 T3802 T3801 arg2Of determined,were
R3141 T3802 T3803 arg1Of determined,in
R3142 T3806 T3803 arg2Of studies,in
R3143 T3806 T3804 arg1Of studies,previous
R3144 T3806 T3805 arg1Of studies,pilot
R3145 T3806 T3807 arg2Of studies,done
R3146 T3807 T3808 arg1Of done,in
R3147 T3810 T3808 arg2Of laboratory,in
R3148 T3810 T3809 arg1Of laboratory,our
R3149 T3810 T3811 arg1Of laboratory,","
R3150 T3810 T3812 arg1Of laboratory,which
R3151 T3810 T3813 arg1Of laboratory,examined
R3152 T3815 T3813 arg2Of effects,examined
R3153 T3815 T3814 arg1Of effects,the
R3154 T3815 T3816 arg1Of effects,of
R3155 T3815 T3821 arg1Of effects,on
R3156 T3818 T3816 arg2Of concentrations,of
R3157 T3818 T3817 arg1Of concentrations,increasing
R3158 T3818 T3819 arg1Of concentrations,of
R3159 T3820 T3819 arg2Of PTX,of
R3160 T3824 T3821 arg2Of production,on
R3161 T3824 T3822 arg1Of production,LPS-induced
R3162 T3824 T3823 arg1Of production,TNF-α
R3163 T3825 T3826 arg1Of LPS,at
R3164 T3825 T3834 arg1Of LPS,at
R3165 T3825 T3840 arg1Of LPS,were
R3166 T3828 T3826 arg2Of concentration,at
R3167 T3828 T3827 arg1Of concentration,a
R3168 T3828 T3829 arg1Of concentration,of
R3169 T3831 T3832 arg1Of μg/mL,and
R3170 T3832 T3829 arg2Of and,of
R3171 T3832 T3830 arg1Of and,1
R3172 T3833 T3832 arg2Of PTX,and
R3173 T3836 T3834 arg2Of concentration,at
R3174 T3836 T3835 arg1Of concentration,a
R3175 T3836 T3837 arg1Of concentration,of
R3176 T3839 T3837 arg2Of mM,of
R3177 T3839 T3838 arg1Of mM,20
R3178 T3843 T3840 arg2Of concentrations,were
R3179 T3843 T3841 arg1Of concentrations,the
R3180 T3843 T3842 arg1Of concentrations,minimum
R3181 T3843 T3844 arg1Of concentrations,necessary
R3182 T3846 T3844 arg2Of produce,necessary
R3183 T3846 T3845 arg1Of produce,to
R3184 T3846 T3857 arg1Of produce,","
R3185 T3846 T3858 arg1Of produce,respectively
R3186 T3846 T3859 arg1Of produce,","
R3187 T3846 T3860 arg1Of produce,in
R3188 T3848 T3849 arg1Of reproducible,and
R3189 T3850 T3849 arg2Of reliable,and
R3190 T3851 T3852 arg1Of up-regulation,and
R3191 T3852 T3846 arg2Of and,produce
R3192 T3852 T3847 arg1Of and,a
R3193 T3852 T3848 arg1Of and,reproducible
R3194 T3852 T3850 arg1Of and,reliable
R3195 T3852 T3854 arg1Of and,in
R3196 T3853 T3852 arg2Of down-regulation,and
R3197 T3856 T3854 arg2Of production,in
R3198 T3856 T3855 arg1Of production,TNF-α
R3199 T3862 T3860 arg2Of assays,in
R3200 T3862 T3861 arg1Of assays,quantitative
R3201 T3862 T3863 arg1Of assays,(
R3202 T3864 T3863 arg2Of data,(
R3203 T3864 T3866 arg2Of data,shown
R3204 T3866 T3865 arg1Of shown,not
R3205 T3867 T3863 arg3Of ),(
R3302 T4023 T4024 arg1Of Effect,of
R3303 T4023 T4026 arg1Of Effect,on
R3304 T4023 T4030 arg1Of Effect,by
R3305 T4025 T4024 arg2Of PTX,of
R3306 T4029 T4026 arg2Of production,on
R3307 T4029 T4027 arg1Of production,LPS-induced
R3308 T4029 T4028 arg1Of production,TNF-α
R3309 T4032 T4030 arg2Of cells,by
R3310 T4032 T4031 arg1Of cells,mononuclear
R3311 T4034 T4033 arg1Of concentration,The
R3323 T4044 T4045 arg1Of media,of
R3324 T4044 T4063 arg1Of media,","
R3325 T4044 T4064 arg1Of media,at
R3326 T4046 T4045 arg2Of cells,of
R3327 T4046 T4047 arg2Of cells,exposed
R3328 T4047 T4048 arg1Of exposed,to
R3329 T4050 T4048 arg2Of treatments,to
R3330 T4050 T4049 arg1Of treatments,the
R3331 T4050 T4051 arg2Of treatments,described
R3332 T4051 T4052 arg1Of described,above
R3333 T4051 T4053 arg1Of described,after
R3334 T4051 T4055 arg1Of described,for
R3335 T4054 T4053 arg2Of stimulation,after
R3336 T4056 T4055 arg2Of up,for
R3337 T4056 T4057 arg1Of up,to
R3338 T4059 T4057 arg2Of maximum,to
R3339 T4059 T4058 arg1Of maximum,a
R3340 T4059 T4060 arg1Of maximum,of
R3341 T4062 T4060 arg2Of hours,of
R3342 T4062 T4061 arg1Of hours,18
R3343 T4066 T4064 arg2Of intervals,at
R3344 T4066 T4065 arg1Of intervals,3-hour
R3345 T4068 T4067 arg1Of results,The
R3346 T4068 T4069 arg1Of results,are
R3347 T4068 T4070 arg2Of results,expressed
R3348 T4070 T4069 arg2Of expressed,are
R3349 T4070 T4071 arg1Of expressed,in
R3350 T4072 T4071 arg2Of pg/mL,in
R3696 T4475 T4476 arg1Of SDS-PAGE,and
R3697 T4476 T4478 arg1Of and,(
R3698 T4477 T4476 arg2Of Immunoblotting,and
R3699 T4479 T4480 arg1Of I-κBα,","
R3700 T4480 T4484 arg1Of ",",and
R3701 T4482 T4480 arg2Of p65,","
R3702 T4482 T4481 arg1Of p65,NF-κB
R3703 T4484 T4478 arg2Of and,(
R3704 T4484 T4483 arg1Of and,","
R3705 T4485 T4484 arg2Of CREB,and
R3706 T4486 T4478 arg3Of ),(
R3707 T4488 T4487 arg2Of stimulation,Following
R3708 T4490 T4491 arg1Of PBMCs,were
R3709 T4490 T4492 arg2Of PBMCs,washed
R3710 T4490 T4497 arg2Of PBMCs,centrifuged
R3711 T4490 T4499 arg1Of PBMCs,collect
R3712 T4492 T4493 arg1Of washed,with
R3713 T4492 T4496 arg1Of washed,and
R3714 T4495 T4493 arg2Of PBS,with
R3715 T4495 T4494 arg1Of PBS,ice-cold
R3716 T4496 T4487 arg1Of and,Following
R3717 T4496 T4489 arg1Of and,","
R3718 T4496 T4491 arg2Of and,were
R3719 T4497 T4496 arg2Of centrifuged,and
R3720 T4499 T4497 arg3Of collect,centrifuged
R3721 T4499 T4498 arg1Of collect,to
R3722 T4502 T4499 arg2Of pellet,collect
R3723 T4502 T4500 arg1Of pellet,a
R3724 T4502 T4501 arg1Of pellet,cell
R3725 T4504 T4503 arg1Of pellet,The
R3726 T4504 T4505 arg1Of pellet,was
R3727 T4504 T4506 arg2Of pellet,resuspended
R3728 T4506 T4505 arg2Of resuspended,was
R3729 T4506 T4507 arg1Of resuspended,in
R3730 T4511 T4507 arg2Of buffer,in
R3731 T4511 T4508 arg1Of buffer,ice-cold
R3732 T4511 T4509 arg1Of buffer,SDS
R3733 T4511 T4510 arg1Of buffer,sample
R3734 T4511 T4512 arg2Of buffer,supplemented
R3735 T4512 T4513 arg1Of supplemented,with
R3736 T4514 T4515 arg1Of 100,mM
R3737 T4516 T4513 arg2Of dithiothreitol,with
R3738 T4516 T4514 arg1Of dithiothreitol,100
R3739 T4517 T4518 arg1Of Cytoplasmic,and
R3740 T4519 T4518 arg2Of nuclear,and
R3741 T4520 T4517 arg1Of extracts,Cytoplasmic
R3742 T4520 T4519 arg1Of extracts,nuclear
R3743 T4520 T4521 arg1Of extracts,were
R3744 T4520 T4522 arg2Of extracts,isolated
R3745 T4522 T4521 arg2Of isolated,were
R3746 T4522 T4523 arg1Of isolated,with
R3747 T4522 T4536 arg1Of isolated,according
R3748 T4525 T4526 arg1Of nuclear,and
R3749 T4527 T4526 arg2Of cytoplasmic,and
R3750 T4529 T4523 arg2Of reagents,with
R3751 T4529 T4524 arg1Of reagents,NE-PER
R3752 T4529 T4525 arg1Of reagents,nuclear
R3753 T4529 T4527 arg1Of reagents,cytoplasmic
R3754 T4529 T4528 arg1Of reagents,extraction
R3755 T4529 T4530 arg1Of reagents,with
R3756 T4535 T4530 arg2Of Cocktail,with
R3757 T4535 T4531 arg1Of Cocktail,1x
R3758 T4535 T4532 arg1Of Cocktail,Halt
R3759 T4535 T4533 arg1Of Cocktail,Protease
R3760 T4535 T4534 arg1Of Cocktail,Inhibitor
R3761 T4537 T4536 arg2Of to,according
R3762 T4540 T4537 arg2Of instructions,to
R3763 T4540 T4538 arg1Of instructions,the
R3764 T4540 T4539 arg1Of instructions,manufacturer’s
R3765 T4542 T4541 arg1Of concentrations,Protein
R3766 T4542 T4543 arg1Of concentrations,were
R3767 T4542 T4544 arg2Of concentrations,determined
R3768 T4544 T4543 arg2Of determined,were
R3769 T4544 T4545 arg1Of determined,with
R3770 T4544 T4554 arg1Of determined,according
R3771 T4550 T4545 arg2Of kit,with
R3772 T4550 T4546 arg1Of kit,the
R3773 T4550 T4547 arg1Of kit,BCA
R3774 T4550 T4548 arg1Of kit,protein
R3775 T4550 T4549 arg1Of kit,reagent
R3776 T4550 T4551 arg1Of kit,for
R3777 T4553 T4551 arg2Of sample,for
R3778 T4553 T4552 arg1Of sample,each
R3779 T4555 T4554 arg2Of to,according
R3780 T4558 T4555 arg2Of curve,to
R3781 T4558 T4556 arg1Of curve,a
R3782 T4558 T4557 arg1Of curve,standardized
R3783 T4558 T4559 arg1Of curve,for
R3784 T4560 T4559 arg2Of albumin,for
R3785 T4562 T4561 arg1Of lysates,Cell
R3786 T4562 T4563 arg1Of lysates,were
R3787 T4562 T4564 arg2Of lysates,collected
R3788 T4564 T4563 arg2Of collected,were
R3789 T4564 T4565 arg1Of collected,by
R3790 T4566 T4565 arg2Of boiling,by
R3791 T4566 T4569 arg1Of boiling,in
R3792 T4568 T4566 arg2Of samples,boiling
R3793 T4568 T4567 arg1Of samples,the
R3794 T4573 T4569 arg2Of bath,in
R3795 T4573 T4570 arg1Of bath,a
R3796 T4573 T4571 arg1Of bath,100ºC
R3797 T4573 T4572 arg1Of bath,water
R3798 T4573 T4574 arg1Of bath,for
R3799 T4576 T4574 arg2Of minutes,for
R3800 T4576 T4575 arg1Of minutes,5
R3801 T4578 T4577 arg1Of μg,Ten
R3802 T4578 T4579 arg1Of μg,of
R3803 T4578 T4581 arg1Of μg,per
R3804 T4578 T4583 arg1Of μg,was
R3805 T4578 T4584 arg2Of μg,separated
R3806 T4578 T4597 arg2Of μg,transferred
R3807 T4580 T4579 arg2Of protein,of
R3808 T4582 T4581 arg2Of sample,per
R3809 T4584 T4589 arg1Of separated,through
R3810 T4584 T4596 arg1Of separated,and
R3811 T4588 T4584 arg1Of electrophoresis,separated
R3812 T4588 T4585 arg2Of electrophoresis,by
R3813 T4588 T4586 arg1Of electrophoresis,SDS-polyacrylamide
R3814 T4588 T4587 arg1Of electrophoresis,gel
R3815 T4590 T4591 arg1Of 8–16,%
R3816 T4595 T4589 arg2Of gels,through
R3817 T4595 T4590 arg1Of gels,8–16
R3818 T4595 T4592 arg1Of gels,tris-glycine
R3819 T4595 T4593 arg1Of gels,polyacrylamide
R3820 T4595 T4594 arg1Of gels,gradient
R3821 T4596 T4583 arg2Of and,was
R3822 T4597 T4596 arg2Of transferred,and
R3823 T4597 T4598 modOf transferred,to
R3824 T4599 T4598 arg1Of nitrocellulose,to
R3825 T4600 T4599 arg2Of membranes,nitrocellulose
R3826 T4602 T4601 arg1Of membranes,The
R3827 T4602 T4603 arg1Of membranes,were
R3828 T4602 T4604 arg2Of membranes,blocked
R3829 T4604 T4603 arg2Of blocked,were
R3830 T4604 T4605 arg1Of blocked,with
R3831 T4606 T4607 arg1Of 5,%
R3832 T4608 T4605 arg2Of milk,with
R3833 T4608 T4606 arg1Of milk,5
R3834 T4608 T4609 arg1Of milk,in
R3835 T4611 T4609 arg2Of saline/Tween,in
R3836 T4611 T4610 arg1Of saline/Tween,Tris-buffered
R3837 T4611 T4612 arg1Of saline/Tween,20
R3838 T4611 T4613 arg1Of saline/Tween,(
R3839 T4611 T4621 arg1Of saline/Tween,for
R3840 T4615 T4613 arg2Of Scientific,(
R3841 T4615 T4614 arg1Of Scientific,Fischer
R3842 T4615 T4616 arg1Of Scientific,","
R3843 T4617 T4616 arg2Of Pittsburgh,","
R3844 T4617 T4618 arg1Of Pittsburgh,","
R3845 T4619 T4618 arg2Of PA,","
R3846 T4620 T4613 arg3Of ),(
R3847 T4623 T4621 arg2Of hour,for
R3848 T4623 T4622 arg1Of hour,1
R3849 T4625 T4624 arg1Of extracts,Cytoplasmic
R3850 T4625 T4626 arg1Of extracts,were
R3851 T4625 T4627 arg2Of extracts,incubated
R3852 T4627 T4626 arg2Of incubated,were
R3853 T4627 T4628 arg1Of incubated,with
R3854 T4627 T4637 arg1Of incubated,","
R3855 T4627 T4638 arg1Of incubated,while
R3856 T4631 T4628 arg2Of antibody,with
R3857 T4631 T4629 arg2Of antibody,phosphorylated
R3858 T4631 T4630 arg1Of antibody,I-κBα
R3859 T4631 T4632 arg1Of antibody,(
R3860 T4633 T4634 arg1Of 1,:
R3861 T4634 T4632 arg2Of :,(
R3862 T4635 T4634 arg2Of 200,:
R3863 T4636 T4632 arg3Of ),(
R3864 T4640 T4639 arg1Of extracts,nuclear
R3865 T4640 T4641 arg1Of extracts,were
R3866 T4640 T4642 arg2Of extracts,incubated
R3867 T4642 T4638 arg2Of incubated,while
R3868 T4642 T4641 arg2Of incubated,were
R3869 T4642 T4643 arg1Of incubated,with
R3870 T4642 T4664 arg1Of incubated,at
R3871 T4648 T4644 arg1Of antibody,either
R3872 T4648 T4645 arg2Of antibody,phosphorylated
R3873 T4648 T4646 arg1Of antibody,NF-κB
R3874 T4648 T4647 arg1Of antibody,p65
R3875 T4648 T4649 arg1Of antibody,(
R3876 T4648 T4654 arg1Of antibody,or
R3877 T4650 T4651 arg1Of 1,:
R3878 T4651 T4649 arg2Of :,(
R3879 T4652 T4651 arg2Of 500,:
R3880 T4653 T4649 arg3Of ),(
R3881 T4654 T4643 arg2Of or,with
R3882 T4657 T4654 arg2Of antibody,or
R3883 T4657 T4655 arg2Of antibody,phosphorylated
R3884 T4657 T4656 arg1Of antibody,CREB
R3885 T4657 T4658 arg1Of antibody,(
R3886 T4657 T4663 arg1Of antibody,overnight
R3887 T4659 T4660 arg1Of 1,:
R3888 T4660 T4658 arg2Of :,(
R3889 T4661 T4660 arg2Of 500,:
R3890 T4662 T4658 arg3Of ),(
R3891 T4665 T4664 arg2Of 4°C,at
R3892 T4665 T4666 arg1Of 4°C,in
R3893 T4668 T4666 arg2Of experiments,in
R3894 T4668 T4667 arg1Of experiments,separate
R3895 T4670 T4669 arg1Of membranes,The
R3896 T4670 T4671 arg1Of membranes,were
R3897 T4670 T4672 arg2Of membranes,washed
R3898 T4670 T4678 arg2Of membranes,incubated
R3899 T4672 T4673 arg1Of washed,with
R3900 T4672 T4677 arg1Of washed,and
R3901 T4675 T4673 arg2Of saline/Tween,with
R3902 T4675 T4674 arg1Of saline/Tween,Tris-buffered
R3903 T4675 T4676 arg1Of saline/Tween,20
R3904 T4677 T4671 arg2Of and,were
R3905 T4678 T4677 arg2Of incubated,and
R3906 T4678 T4679 arg1Of incubated,for
R3907 T4678 T4682 arg1Of incubated,at
R3908 T4681 T4679 arg2Of hour,for
R3909 T4681 T4680 arg1Of hour,1
R3910 T4684 T4682 arg2Of temperature,at
R3911 T4684 T4683 arg1Of temperature,room
R3912 T4684 T4685 arg1Of temperature,with
R3913 T4688 T4685 arg2Of antibody,with
R3914 T4688 T4686 arg1Of antibody,the
R3915 T4688 T4687 arg1Of antibody,secondary
R3916 T4693 T4690 arg1Of IgG,horseradish
R3917 T4693 T4691 arg1Of IgG,peroxidase-linked
R3918 T4693 T4692 arg1Of IgG,anti-rabbit
R3919 T4693 T4694 arg1Of IgG,diluted
R3920 T4694 T4671 modOf diluted,were
R3921 T4694 T4689 arg1Of diluted,","
R3922 T4694 T4696 arg1Of diluted,:
R3923 T4695 T4694 arg2Of 1,diluted
R3924 T4697 T4694 arg3Of 2000,diluted
R3925 T4697 T4698 arg1Of 2000,in
R3926 T4699 T4698 arg2Of blocking,in
R3927 T4700 T4699 arg2Of solution,blocking
R3928 T4703 T4701 arg2Of washing,After
R3929 T4703 T4702 arg2Of washing,repeated
R3930 T4703 T4704 arg1Of washing,of
R3931 T4706 T4704 arg2Of membrane,of
R3932 T4706 T4705 arg1Of membrane,the
R3933 T4713 T4708 arg1Of Kit,the
R3934 T4713 T4709 arg1Of Kit,Supersignal
R3935 T4713 T4710 arg1Of Kit,West
R3936 T4713 T4711 arg1Of Kit,Pico
R3937 T4713 T4712 arg1Of Kit,Chemiluminescent
R3938 T4713 T4714 arg1Of Kit,was
R3939 T4713 T4715 arg2Of Kit,applied
R3940 T4715 T4701 arg1Of applied,After
R3941 T4715 T4707 arg1Of applied,","
R3942 T4715 T4714 arg2Of applied,was
R3943 T4715 T4716 arg1Of applied,for
R3944 T4718 T4716 arg2Of detection,for
R3945 T4718 T4717 arg1Of detection,antibody
R3946 T4718 T4719 arg1Of detection,per
R3947 T4722 T4719 arg2Of instructions,per
R3948 T4722 T4720 arg1Of instructions,the
R3949 T4722 T4721 arg1Of instructions,manufacturer’s
R3950 T4725 T4723 arg1Of data,Western
R3951 T4725 T4724 arg1Of data,blot
R3952 T4725 T4726 arg1Of data,is
R3953 T4725 T4727 arg2Of data,presented
R3954 T4727 T4726 arg2Of presented,is
R3955 T4727 T4728 arg1Of presented,as
R3956 T4730 T4728 arg2Of percentage,as
R3957 T4730 T4729 arg1Of percentage,a
R3958 T4730 T4731 arg1Of percentage,of
R3959 T4733 T4731 arg2Of stimulation,of
R3960 T4733 T4732 arg1Of stimulation,LPS
R3961 T4735 T4734 arg1Of percentage,The
R3962 T4735 T4736 arg1Of percentage,of
R3963 T4735 T4739 arg1Of percentage,was
R3964 T4735 T4740 arg2Of percentage,calculated
R3965 T4738 T4736 arg2Of stimulation,of
R3966 T4738 T4737 arg1Of stimulation,LPS
R3967 T4740 T4739 arg2Of calculated,was
R3968 T4740 T4741 arg1Of calculated,by
R3969 T4742 T4741 arg2Of dividing,by
R3970 T4747 T4742 arg2Of total,dividing
R3971 T4747 T4743 arg1Of total,the
R3972 T4747 T4744 arg1Of total,mean
R3973 T4747 T4745 arg1Of total,band
R3974 T4747 T4746 arg1Of total,pixel
R3975 T4747 T4748 arg1Of total,for
R3976 T4751 T4748 arg2Of arm,for
R3977 T4751 T4749 arg1Of arm,each
R3978 T4751 T4750 arg1Of arm,treatment
R3979 T4751 T4752 arg2Of arm,divided
R3980 T4757 T4752 arg1Of total,divided
R3981 T4757 T4753 arg2Of total,by
R3982 T4757 T4754 arg1Of total,the
R3983 T4757 T4755 arg1Of total,mean
R3984 T4757 T4756 arg1Of total,pixel
R3985 T4757 T4758 arg1Of total,of
R3986 T4759 T4758 arg2Of samples,of
R3987 T4759 T4760 arg2Of samples,stimulated
R3988 T4759 T4764 arg1Of samples,multiplying
R3989 T4760 T4761 arg1Of stimulated,with
R3990 T4760 T4763 arg1Of stimulated,and
R3991 T4762 T4761 arg2Of LPS,with
R3992 T4764 T4763 arg2Of multiplying,and
R3993 T4764 T4765 arg1Of multiplying,by
R3994 T4766 T4765 arg2Of 100,by
R3995 T4770 T4769 arg1Of stimulation,LPS
R3996 T4770 T4771 arg1Of stimulation,is
R3997 T4770 T4772 arg2Of stimulation,reported
R3998 T4772 T4771 arg2Of reported,is
R3999 T4772 T4773 arg1Of reported,as
R4000 T4772 T4776 arg1Of reported,and
R4001 T4775 T4773 arg2Of %,as
R4002 T4775 T4774 arg1Of %,100
R4003 T4776 T4767 arg1Of and,Thus
R4004 T4776 T4768 arg1Of and,","
R4005 T4777 T4778 arg1Of each,of
R4006 T4777 T4782 arg1Of each,is
R4007 T4777 T4783 arg2Of each,reported
R4008 T4781 T4778 arg2Of arms,of
R4009 T4781 T4779 arg1Of arms,the
R4010 T4781 T4780 arg1Of arms,treatment
R4011 T4783 T4776 arg2Of reported,and
R4012 T4783 T4782 arg2Of reported,is
R4013 T4783 T4784 arg1Of reported,as
R4014 T4786 T4784 arg2Of percent,as
R4015 T4786 T4785 arg1Of percent,a
R4016 T4786 T4787 arg1Of percent,of
R4017 T4789 T4787 arg2Of stimulation,of
R4018 T4789 T4788 arg1Of stimulation,LPS
R4358 T5231 T5232 arg1Of NF-κB,and
R4359 T5233 T5232 arg2Of CREB,and
R4360 T5237 T5231 arg1Of Assay,NF-κB
R4361 T5237 T5233 arg1Of Assay,CREB
R4362 T5237 T5234 arg1Of Assay,Electrophoretic
R4363 T5237 T5235 arg1Of Assay,Mobility
R4364 T5237 T5236 arg1Of Assay,Shift
R4365 T5237 T5238 arg1Of Assay,(
R4366 T5239 T5238 arg2Of EMSA,(
R4367 T5240 T5238 arg3Of ),(
R4368 T5245 T5243 arg1Of EMSA,LightShift
R4369 T5245 T5244 arg1Of EMSA,Chemiluminescent
R4370 T5246 T5241 arg1Of Kit,The
R4371 T5246 T5242 arg1Of Kit,non-radioactive
R4372 T5246 T5245 arg1Of Kit,EMSA
R4373 T5246 T5247 arg1Of Kit,was
R4374 T5246 T5248 arg2Of Kit,used
R4375 T5248 T5247 arg2Of used,was
R4376 T5248 T5249 modOf used,to
R4377 T5250 T5249 arg1Of detect,to
R4378 T5252 T5250 arg2Of interactions,detect
R4379 T5252 T5251 arg1Of interactions,DNA-protein
R4380 T5259 T5253 arg1Of oligonucleotide,The
R4381 T5259 T5254 arg1Of oligonucleotide,NF-κB
R4382 T5259 T5255 arg1Of oligonucleotide,3′
R4383 T5259 T5256 arg1Of oligonucleotide,biotin
R4384 T5259 T5257 arg1Of oligonucleotide,end-labeled
R4385 T5259 T5258 arg1Of oligonucleotide,DNA
R4386 T5259 T5260 arg2Of oligonucleotide,used
R4387 T5259 T5267 arg1Of oligonucleotide,was
R4388 T5260 T5261 arg1Of used,as
R4389 T5263 T5261 arg2Of probe,as
R4390 T5263 T5262 arg1Of probe,a
R4391 T5263 T5264 arg1Of probe,for
R4392 T5266 T5264 arg2Of EMSA,for
R4393 T5266 T5265 arg1Of EMSA,the
R4394 T5272 T5267 arg2Of construct,was
R4395 T5272 T5268 arg1Of construct,a
R4396 T5272 T5269 arg1Of construct,42-bp
R4397 T5272 T5270 arg1Of construct,double
R4398 T5272 T5271 arg1Of construct,stranded
R4399 T5272 T5273 arg1Of construct,(
R4400 T5272 T5278 arg1Of construct,containing
R4401 T5276 T5273 arg2Of 3′,(
R4402 T5276 T5274 arg1Of 3′,5′-TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4403 T5276 T5275 arg1Of 3′,–
R4404 T5277 T5273 arg3Of ),(
R4405 T5284 T5278 arg2Of sites,containing
R4406 T5284 T5279 arg1Of sites,two
R4407 T5284 T5280 arg1Of sites,tandemly
R4408 T5284 T5281 arg2Of sites,repeated
R4409 T5284 T5282 arg1Of sites,NF-κB
R4410 T5284 T5283 arg1Of sites,binding
R4411 T5284 T5285 arg1Of sites,(
R4412 T5286 T5285 arg2Of underlined,(
R4413 T5287 T5285 arg3Of ),(
R4414 T5288 T5289 arg1Of Specificity,was
R4415 T5288 T5290 arg2Of Specificity,determined
R4416 T5290 T5289 arg2Of determined,was
R4417 T5294 T5290 arg1Of assay,determined
R4418 T5294 T5291 arg2Of assay,by
R4419 T5294 T5292 arg1Of assay,a
R4420 T5294 T5293 arg1Of assay,competition
R4421 T5294 T5295 arg1Of assay,with
R4422 T5297 T5295 arg2Of addition,with
R4423 T5297 T5296 arg1Of addition,the
R4424 T5297 T5298 arg1Of addition,of
R4425 T5300 T5299 arg1Of molar,200
R4426 T5301 T5298 arg2Of excess,of
R4427 T5301 T5300 arg1Of excess,molar
R4428 T5301 T5302 arg1Of excess,of
R4429 T5305 T5303 arg1Of stranded,unlabeled
R4430 T5305 T5304 arg1Of stranded,double
R4431 T5307 T5302 arg2Of oligonucleotide,of
R4432 T5307 T5305 arg1Of oligonucleotide,stranded
R4433 T5307 T5306 arg1Of oligonucleotide,NF-κB
R4434 T5314 T5308 arg1Of oligonucleotide,The
R4435 T5314 T5309 arg1Of oligonucleotide,CREB
R4436 T5314 T5310 arg1Of oligonucleotide,3′
R4437 T5314 T5311 arg1Of oligonucleotide,biotin
R4438 T5314 T5312 arg1Of oligonucleotide,end-labeled
R4439 T5314 T5313 arg1Of oligonucleotide,DNA
R4440 T5314 T5315 arg2Of oligonucleotide,used
R4441 T5314 T5322 arg1Of oligonucleotide,was
R4442 T5315 T5316 arg1Of used,as
R4443 T5318 T5316 arg2Of probe,as
R4444 T5318 T5317 arg1Of probe,a
R4445 T5318 T5319 arg1Of probe,for
R4446 T5321 T5319 arg2Of EMSA,for
R4447 T5321 T5320 arg1Of EMSA,the
R4448 T5327 T5322 arg2Of construct,was
R4449 T5327 T5323 arg1Of construct,a
R4450 T5327 T5324 arg1Of construct,23-bp
R4451 T5327 T5325 arg1Of construct,double
R4452 T5327 T5326 arg1Of construct,stranded
R4453 T5327 T5328 arg1Of construct,(
R4454 T5327 T5336 arg1Of construct,containing
R4455 T5334 T5328 arg2Of 3′,(
R4456 T5334 T5329 arg1Of 3′,5′-TTT
R4457 T5334 T5330 arg1Of 3′,TCG
R4458 T5334 T5331 arg1Of 3′,AGC
R4459 T5334 T5332 arg1Of 3′,TCTGACGTCAGA-GC
R4460 T5334 T5333 arg1Of 3′,–
R4461 T5335 T5328 arg3Of ),(
R4462 T5340 T5336 arg2Of sequence,containing
R4463 T5340 T5337 arg1Of sequence,the
R4464 T5340 T5338 arg1Of sequence,CRE
R4465 T5340 T5339 arg1Of sequence,consensus
R4466 T5340 T5341 arg1Of sequence,(
R4467 T5342 T5341 arg2Of underlined,(
R4468 T5343 T5341 arg3Of ),(
R4469 T5344 T5345 arg1Of Specificity,was
R4470 T5344 T5346 arg2Of Specificity,determined
R4471 T5346 T5345 arg2Of determined,was
R4472 T5350 T5346 arg1Of assay,determined
R4473 T5350 T5347 arg2Of assay,by
R4474 T5350 T5348 arg1Of assay,a
R4475 T5350 T5349 arg1Of assay,competition
R4476 T5350 T5351 arg1Of assay,with
R4477 T5353 T5351 arg2Of addition,with
R4478 T5353 T5352 arg1Of addition,the
R4479 T5353 T5354 arg1Of addition,of
R4480 T5356 T5355 arg1Of molar,200
R4481 T5357 T5354 arg2Of excess,of
R4482 T5357 T5356 arg1Of excess,molar
R4483 T5357 T5358 arg1Of excess,of
R4484 T5361 T5359 arg1Of stranded,unlabeled
R4485 T5361 T5360 arg1Of stranded,double
R4486 T5363 T5358 arg2Of oligonucleotide,of
R4487 T5363 T5361 arg1Of oligonucleotide,stranded
R4488 T5363 T5362 arg1Of oligonucleotide,CREB
R4489 T5365 T5364 arg1Of extracts,Nuclear
R4490 T5365 T5366 arg1Of extracts,(
R4491 T5365 T5370 arg1Of extracts,were
R4492 T5365 T5371 arg2Of extracts,incubated
R4493 T5368 T5366 arg2Of μg,(
R4494 T5368 T5367 arg1Of μg,10
R4495 T5369 T5366 arg3Of ),(
R4496 T5371 T5370 arg2Of incubated,were
R4497 T5371 T5372 arg1Of incubated,with
R4498 T5373 T5374 arg1Of 5,nM
R4499 T5375 T5373 arg1Of NF-κB,5
R4500 T5375 T5376 arg1Of NF-κB,or
R4501 T5376 T5372 arg2Of or,with
R4502 T5378 T5376 arg2Of probe,or
R4503 T5378 T5377 arg1Of probe,CREB
R4504 T5378 T5379 arg1Of probe,(
R4505 T5380 T5379 arg2Of NF-kB,(
R4506 T5381 T5379 arg3Of :,(
R4507 T5384 T5382 arg1Of buffer,1x
R4508 T5384 T5383 arg1Of buffer,binding
R4509 T5384 T5385 arg1Of buffer,","
R4510 T5385 T5389 arg1Of ",",","
R4511 T5386 T5387 arg1Of 50,mM
R4512 T5388 T5385 arg2Of KCl,","
R4513 T5388 T5386 arg1Of KCl,50
R4514 T5389 T5393 arg1Of ",",","
R4515 T5390 T5391 arg1Of 1,mM
R4516 T5392 T5389 arg2Of EDTA,","
R4517 T5392 T5390 arg1Of EDTA,1
R4518 T5393 T5397 arg1Of ",",","
R4519 T5394 T5395 arg1Of 1,mM
R4520 T5396 T5393 arg2Of DTT,","
R4521 T5396 T5394 arg1Of DTT,1
R4522 T5397 T5401 arg1Of ",",","
R4523 T5398 T5399 arg1Of 0.1,%
R4524 T5400 T5397 arg2Of NP40,","
R4525 T5400 T5398 arg1Of NP40,0.1
R4526 T5401 T5406 arg1Of ",",and
R4527 T5403 T5402 arg1Of %,10
R4528 T5404 T5401 arg2Of glycerol,","
R4529 T5404 T5403 arg1Of glycerol,%
R4530 T5406 T5405 arg1Of and,","
R4531 T5410 T5406 arg2Of dI-dC,and
R4532 T5410 T5407 arg1Of dI-dC,50
R4533 T5410 T5408 arg1Of dI-dC,ng/μl
R4534 T5410 T5409 arg1Of dI-dC,poly
R4535 T5410 T5411 arg1Of dI-dC,","
R4536 T5412 T5411 arg2Of CREB,","
R4537 T5412 T5413 arg1Of CREB,:
R4538 T5416 T5414 arg1Of buffer,1x
R4539 T5416 T5415 arg1Of buffer,binding
R4540 T5416 T5417 arg1Of buffer,","
R4541 T5418 T5419 arg1Of 20,mM
R4542 T5420 T5417 arg2Of Tris,","
R4543 T5420 T5418 arg1Of Tris,20
R4544 T5420 T5421 arg1Of Tris,","
R4545 T5422 T5421 arg2Of pH,","
R4546 T5422 T5423 arg1Of pH,7.5
R4547 T5425 T5426 arg1Of 50,mM
R4548 T5427 T5425 arg1Of KCl,50
R4549 T5427 T5428 arg1Of KCl,","
R4550 T5428 T5432 arg1Of ",",","
R4551 T5429 T5430 arg1Of 1,mM
R4552 T5431 T5428 arg2Of EDTA,","
R4553 T5431 T5429 arg1Of EDTA,1
R4554 T5433 T5434 arg1Of 1,mM
R4555 T5435 T5432 arg2Of DTT,","
R4556 T5435 T5433 arg1Of DTT,1
R4557 T5437 T5438 arg1Of 0.10,%
R4558 T5439 T5437 arg1Of NP40,0.10
R4559 T5439 T5440 arg1Of NP40,","
R4560 T5440 T5444 arg1Of ",",","
R4561 T5441 T5442 arg1Of 6,%
R4562 T5443 T5440 arg2Of glycerol,","
R4563 T5443 T5441 arg1Of glycerol,6
R4564 T5444 T5448 arg1Of ",",and
R4565 T5445 T5446 arg1Of 0.1,mg/mL
R4566 T5447 T5444 arg2Of BSA,","
R4567 T5447 T5445 arg1Of BSA,0.1
R4568 T5452 T5448 arg2Of dI-dC,and
R4569 T5452 T5449 arg1Of dI-dC,50
R4570 T5452 T5450 arg1Of dI-dC,ng/μl
R4571 T5452 T5451 arg1Of dI-dC,poly
R4572 T5457 T5454 arg2Of electrophoresed,and
R4573 T5457 T5455 arg2Of electrophoresed,were
R4574 T5457 T5456 arg1Of electrophoresed,then
R4575 T5457 T5458 arg1Of electrophoresed,through
R4576 T5460 T5461 arg1Of 6,%
R4577 T5464 T5458 arg2Of gel,through
R4578 T5464 T5459 arg1Of gel,a
R4579 T5464 T5460 arg1Of gel,6
R4580 T5464 T5462 arg1Of gel,DNA
R4581 T5464 T5463 arg1Of gel,retardation
R4582 T5464 T5465 arg1Of gel,at
R4583 T5466 T5465 arg2Of 100V,at
R4584 T5466 T5467 arg1Of 100V,for
R4585 T5469 T5467 arg2Of minutes,for
R4586 T5469 T5468 arg1Of minutes,90
R4587 T5471 T5470 arg1Of gels,The
R4588 T5471 T5472 arg1Of gels,were
R4589 T5471 T5474 arg2Of gels,transferred
R4590 T5474 T5472 arg2Of transferred,were
R4591 T5474 T5473 arg1Of transferred,electrophoretically
R4592 T5474 T5475 arg1Of transferred,at
R4593 T5476 T5475 arg2Of 380mA,at
R4594 T5476 T5477 arg1Of 380mA,for
R4595 T5476 T5489 arg1Of 380mA,immediately
R4596 T5477 T5488 arg1Of for,and
R4597 T5479 T5477 arg2Of hour,for
R4598 T5479 T5478 arg1Of hour,1
R4599 T5479 T5480 arg1Of hour,on
R4600 T5479 T5482 arg1Of hour,to
R4601 T5481 T5480 arg2Of ice,on
R4602 T5485 T5484 arg1Of charged,positively
R4603 T5487 T5482 arg2Of membrane,to
R4604 T5487 T5483 arg1Of membrane,a
R4605 T5487 T5485 arg1Of membrane,charged
R4606 T5487 T5486 arg1Of membrane,nylon
R4607 T5489 T5488 arg2Of immediately,and
R4608 T5489 T5490 arg1Of immediately,cross-linked
R4609 T5490 T5491 arg1Of cross-linked,for
R4610 T5493 T5491 arg2Of minutes,for
R4611 T5493 T5492 arg1Of minutes,15
R4612 T5493 T5494 arg1Of minutes,with
R4613 T5497 T5494 arg2Of transilluminator,with
R4614 T5497 T5495 arg1Of transilluminator,a
R4615 T5497 T5496 arg1Of transilluminator,UV
R4616 T5497 T5498 arg2Of transilluminator,equipped
R4617 T5498 T5499 arg1Of equipped,with
R4618 T5501 T5502 arg1Of 312,nm
R4619 T5503 T5499 arg2Of bulb,with
R4620 T5503 T5500 arg1Of bulb,a
R4621 T5503 T5501 arg1Of bulb,312
R4622 T5506 T5504 arg1Of conjugate,Streptavidin-horseradish
R4623 T5506 T5505 arg1Of conjugate,peroxidase
R4624 T5506 T5507 arg1Of conjugate,and
R4625 T5507 T5512 arg1Of and,were
R4626 T5507 T5513 arg2Of and,used
R4627 T5511 T5507 arg2Of Substrate,and
R4628 T5511 T5508 arg1Of Substrate,the
R4629 T5511 T5509 arg1Of Substrate,LightShift
R4630 T5511 T5510 arg1Of Substrate,Chemiluminescent
R4631 T5513 T5512 arg2Of used,were
R4632 T5515 T5513 arg3Of detect,used
R4633 T5515 T5514 arg1Of detect,to
R4634 T5519 T5515 arg2Of DNA,detect
R4635 T5519 T5516 arg1Of DNA,the
R4636 T5519 T5517 arg1Of DNA,biotin
R4637 T5519 T5518 arg1Of DNA,end-labeled
R4638 T5522 T5520 arg1Of membranes,The
R4639 T5522 T5521 arg1Of membranes,nylon
R4640 T5522 T5523 arg1Of membranes,were
R4641 T5522 T5524 arg2Of membranes,exposed
R4642 T5524 T5523 arg2Of exposed,were
R4643 T5524 T5525 arg1Of exposed,to
R4644 T5524 T5528 arg1Of exposed,for
R4645 T5527 T5525 arg2Of film,to
R4646 T5527 T5526 arg1Of film,x-ray
R4647 T5530 T5528 arg2Of minutes,for
R4648 T5530 T5529 arg1Of minutes,1–3
R4649 T5530 T5531 arg1Of minutes,for
R4650 T5532 T5531 arg2Of detection,for
R4831 T5799 T5800 arg1Of Role,of
R4832 T5799 T5802 arg1Of Role,on
R4833 T5801 T5800 arg2Of PKA,of
R4834 T5807 T5802 arg2Of production,on
R4835 T5807 T5803 arg1Of production,LPS-induced
R4836 T5807 T5804 arg1Of production,mononuclear
R4837 T5807 T5805 arg1Of production,cell
R4838 T5807 T5806 arg1Of production,TNF-α
R4839 T5809 T5808 arg1Of evaluate,To
R4840 T5809 T5842 arg1Of evaluate,was
R4841 T5809 T5843 arg2Of evaluate,utilized
R4842 T5811 T5810 arg1Of role,the
R4843 T5811 T5812 arg1Of role,of
R4844 T5811 T5814 arg1Of role,on
R4845 T5811 T5817 arg1Of role,in
R4846 T5811 T5820 arg1Of role,and
R4847 T5813 T5812 arg2Of PKA,of
R4848 T5816 T5814 arg2Of production,on
R4849 T5816 T5815 arg1Of production,TNF-α
R4850 T5819 T5817 arg2Of cells,in
R4851 T5819 T5818 arg1Of cells,mononuclear
R4852 T5820 T5809 arg2Of and,evaluate
R4853 T5822 T5820 arg2Of involvement,and
R4854 T5822 T5821 arg1Of involvement,its
R4855 T5822 T5823 arg1Of involvement,in
R4856 T5825 T5823 arg2Of attenuation,in
R4857 T5825 T5824 arg1Of attenuation,the
R4858 T5825 T5826 arg1Of attenuation,of
R4859 T5829 T5826 arg2Of production,of
R4860 T5829 T5827 arg1Of production,LPS-induced
R4861 T5829 T5828 arg1Of production,TNF-α
R4862 T5829 T5830 arg2Of production,observed
R4863 T5830 T5831 arg1Of observed,following
R4864 T5833 T5831 arg2Of treatment,following
R4865 T5833 T5832 arg1Of treatment,PTX
R4866 T5833 T5834 arg1Of treatment,","
R4867 T5833 T5836 arg1Of treatment,","
R4868 T5835 T5834 arg2Of H89,","
R4869 T5840 T5836 arg2Of inhibitor,","
R4870 T5840 T5837 arg1Of inhibitor,a
R4871 T5840 T5838 arg1Of inhibitor,specific
R4872 T5840 T5839 arg1Of inhibitor,PKA
R4873 T5843 T5841 arg1Of utilized,","
R4874 T5843 T5842 arg2Of utilized,was
R4875 T5846 T5844 arg2Of cells,Isolated
R4876 T5846 T5845 arg1Of cells,mononuclear
R4877 T5846 T5847 arg1Of cells,were
R4878 T5846 T5848 arg2Of cells,incubated
R4879 T5848 T5847 arg2Of incubated,were
R4880 T5848 T5849 arg1Of incubated,according
R4881 T5850 T5849 arg2Of to,according
R4882 T5853 T5850 arg2Of groups,to
R4883 T5853 T5851 arg1Of groups,the
R4884 T5853 T5852 arg1Of groups,treatment
R4885 T5853 T5854 arg2Of groups,described
R4886 T5854 T5855 arg1Of described,above
R4887 T5854 T5856 arg1Of described,in
R4888 T5858 T5859 arg1Of presence,and
R4889 T5859 T5856 arg2Of and,in
R4890 T5859 T5857 arg1Of and,the
R4891 T5859 T5861 arg1Of and,of
R4892 T5859 T5863 arg1Of and,with
R4893 T5860 T5859 arg2Of absence,and
R4894 T5862 T5861 arg2Of pretreatment,of
R4895 T5864 T5863 arg2Of H89,with
R4896 T5864 T5865 arg1Of H89,(
R4897 T5864 T5869 arg1Of H89,for
R4898 T5867 T5865 arg2Of μM,(
R4899 T5867 T5866 arg1Of μM,10
R4900 T5868 T5865 arg3Of ),(
R4901 T5871 T5869 arg2Of hour,for
R4902 T5871 T5870 arg1Of hour,1
R4903 T5871 T5872 arg1Of hour,at
R4904 T5873 T5872 arg2Of 37ºC,at
R4905 T5875 T5874 arg1Of dose,The
R4906 T5875 T5876 arg1Of dose,of
R4907 T5875 T5878 arg1Of dose,was
R4908 T5875 T5879 arg2Of dose,chosen
R4909 T5877 T5876 arg2Of H89,of
R4910 T5879 T5878 arg2Of chosen,was
R4911 T5879 T5880 modOf chosen,based
R4912 T5879 T5881 arg1Of chosen,on
R4913 T5883 T5881 arg2Of work,on
R4914 T5883 T5882 arg1Of work,previous
R4915 T5883 T5884 arg1Of work,which
R4916 T5883 T5885 arg1Of work,demonstrated
R4917 T5886 T5887 arg1Of specific,and
R4918 T5888 T5887 arg2Of complete,and
R4919 T5889 T5885 arg2Of inhibition,demonstrated
R4920 T5889 T5886 arg1Of inhibition,specific
R4921 T5889 T5888 arg1Of inhibition,complete
R4922 T5889 T5890 arg1Of inhibition,of
R4923 T5891 T5890 arg2Of PKA,of
R4924 T5894 T5892 arg2Of concentration.10,at
R4925 T5894 T5893 arg1Of concentration.10,this
R4926 T5894 T5895 arg1Of concentration.10,Since
R4927 T5897 T5896 arg1Of activity,the
R4928 T5897 T5898 arg1Of activity,of
R4929 T5897 T5901 arg1Of activity,is
R4930 T5897 T5903 arg1Of activity,specific
R4931 T5897 T5905 arg1Of activity,consistent
R4932 T5900 T5898 arg2Of inhibitor,of
R4933 T5900 T5899 arg1Of inhibitor,this
R4934 T5901 T5895 arg2Of is,Since
R4935 T5903 T5904 arg1Of specific,and
R4936 T5904 T5901 arg2Of and,is
R4937 T5904 T5902 arg1Of and,both
R4938 T5904 T5906 arg1Of and,at
R4939 T5905 T5904 arg2Of consistent,and
R4940 T5909 T5906 arg2Of dose,at
R4941 T5909 T5907 arg1Of dose,this
R4942 T5909 T5908 arg2Of dose,specified
R4943 T5911 T5912 arg1Of we,did
R4944 T5911 T5914 arg1Of we,include
R4945 T5914 T5885 arg3Of include,demonstrated
R4946 T5914 T5892 arg1Of include,at
R4947 T5914 T5910 arg1Of include,","
R4948 T5914 T5912 arg2Of include,did
R4949 T5914 T5913 arg1Of include,not
R4950 T5915 T5914 arg2Of experiments,include
R4951 T5915 T5916 arg1Of experiments,with
R4952 T5919 T5916 arg2Of inhibitors,with
R4953 T5919 T5917 arg1Of inhibitors,additional
R4954 T5919 T5918 arg1Of inhibitors,PKA
R4955 T5919 T5920 arg1Of inhibitors,with
R4956 T5922 T5920 arg2Of set,with
R4957 T5922 T5921 arg1Of set,this
R4958 T5922 T5923 arg1Of set,of
R4959 T5924 T5923 arg2Of experiments,of
R4960 T5927 T5925 arg1Of concentration,The
R4961 T5927 T5926 arg1Of concentration,TNF-α
R4962 T5927 T5928 arg1Of concentration,in
R4963 T5927 T5931 arg1Of concentration,was
R4964 T5927 T5932 arg2Of concentration,measured
R4965 T5930 T5928 arg2Of supernatant,in
R4966 T5930 T5929 arg1Of supernatant,the
R4967 T5932 T5931 arg2Of measured,was
R4968 T5932 T5933 arg1Of measured,quantitatively
R4969 T5935 T5932 arg1Of ELISA,measured
R4970 T5935 T5934 arg2Of ELISA,by
R4971 T5937 T5936 arg1Of results,The
R4972 T5937 T5938 arg1Of results,are
R4973 T5937 T5939 arg2Of results,expressed
R4974 T5939 T5938 arg2Of expressed,are
R4975 T5939 T5940 arg1Of expressed,in
R4976 T5941 T5940 arg2Of pg/mL,in
R5103 T6141 T6140 arg1Of Analysis,Statistical
R5104 T6144 T6142 arg1Of results,The
R5105 T6144 T6143 arg1Of results,experimental
R5106 T6144 T6145 arg2Of results,obtained
R5107 T6144 T6149 arg1Of results,were
R5108 T6144 T6150 arg2Of results,derived
R5109 T6145 T6146 arg1Of obtained,in
R5110 T6148 T6146 arg2Of study,in
R5111 T6148 T6147 arg1Of study,this
R5112 T6150 T6149 arg2Of derived,were
R5113 T6150 T6151 arg1Of derived,from
R5114 T6154 T6151 arg2Of experiments,from
R5115 T6154 T6152 arg1Of experiments,four
R5116 T6154 T6153 arg1Of experiments,separate
R5117 T6154 T6155 arg1Of experiments,with
R5118 T6158 T6155 arg2Of donors,with
R5119 T6158 T6156 arg1Of donors,healthy
R5120 T6158 T6157 arg1Of donors,volunteer
R5121 T6160 T6159 arg1Of assay,Each
R5122 T6160 T6161 arg1Of assay,was
R5123 T6160 T6162 arg2Of assay,performed
R5124 T6160 T6166 arg1Of assay,triplicate
R5125 T6162 T6161 arg2Of performed,was
R5126 T6162 T6163 arg1Of performed,in
R5127 T6162 T6165 arg1Of performed,or
R5128 T6164 T6163 arg2Of duplicate,in
R5129 T6166 T6165 arg2Of triplicate,or
R5130 T6166 T6167 arg1Of triplicate,where
R5131 T6168 T6167 arg2Of appropriate,where
R5132 T6169 T6170 arg1Of Data,is
R5133 T6169 T6171 arg2Of Data,presented
R5134 T6171 T6170 arg2Of presented,is
R5135 T6171 T6172 arg1Of presented,as
R5136 T6176 T6172 arg2Of SEM,as
R5137 T6176 T6173 arg1Of SEM,the
R5138 T6176 T6174 arg1Of SEM,mean
R5139 T6176 T6175 arg1Of SEM,±
R5140 T6178 T6177 arg1Of differences,Statistical
R5141 T6178 T6179 arg1Of differences,between
R5142 T6178 T6181 arg1Of differences,were
R5143 T6178 T6182 arg2Of differences,determined
R5144 T6180 T6179 arg2Of groups,between
R5145 T6182 T6181 arg2Of determined,were
R5146 T6185 T6182 arg1Of analysis,determined
R5147 T6185 T6183 arg2Of analysis,by
R5148 T6185 T6184 arg1Of analysis,one-way
R5149 T6185 T6186 arg1Of analysis,of
R5150 T6185 T6191 arg1Of analysis,with
R5151 T6187 T6186 arg2Of variance,of
R5152 T6187 T6188 arg1Of variance,(
R5153 T6189 T6188 arg2Of ANOVA,(
R5154 T6190 T6188 arg3Of ),(
R5155 T6194 T6191 arg2Of correction,with
R5156 T6194 T6192 arg1Of correction,a
R5157 T6194 T6193 arg1Of correction,Bonferroni
R5158 T6196 T6195 arg1Of significance,Statistical
R5159 T6196 T6197 arg1Of significance,was
R5160 T6196 T6198 arg2Of significance,defined
R5161 T6198 T6197 arg2Of defined,was
R5162 T6198 T6199 arg1Of defined,as
R5163 T6202 T6199 arg2Of 0.05,as
R5164 T6202 T6200 arg1Of 0.05,P
R5165 T6202 T6201 arg1Of 0.05,<
R5237 T6294 T6293 arg1Of effects,The
R5238 T6294 T6295 arg1Of effects,of
R5239 T6294 T6297 arg1Of effects,on
R5240 T6296 T6295 arg2Of PTX,of
R5241 T6300 T6297 arg2Of production,on
R5242 T6300 T6298 arg1Of production,LPS-induced
R5243 T6300 T6299 arg1Of production,TNF-α
R5244 T6302 T6301 arg1Of determine,To
R5245 T6302 T6311 arg1Of determine,after
R5246 T6304 T6302 arg2Of duration,determine
R5247 T6304 T6303 arg1Of duration,the
R5248 T6304 T6305 arg1Of duration,of
R5249 T6304 T6308 arg1Of duration,on
R5250 T6307 T6305 arg2Of inhibition,of
R5251 T6307 T6306 arg1Of inhibition,PDE
R5252 T6310 T6308 arg2Of production,on
R5253 T6310 T6309 arg1Of production,TNF-α
R5254 T6313 T6311 arg2Of stimulation,after
R5255 T6313 T6312 arg1Of stimulation,LPS
R5256 T6317 T6315 arg1Of studies,time
R5257 T6317 T6316 arg1Of studies,course
R5258 T6317 T6318 arg1Of studies,were
R5259 T6317 T6319 arg2Of studies,conducted
R5260 T6319 T6301 modOf conducted,To
R5261 T6319 T6314 arg1Of conducted,","
R5262 T6319 T6318 arg2Of conducted,were
R5263 T6321 T6320 arg2Of accordance,In
R5264 T6321 T6322 arg1Of accordance,with
R5265 T6324 T6322 arg2Of work,with
R5266 T6324 T6323 arg1Of work,prior
R5267 T6326 T6327 arg1Of PTX,had
R5268 T6327 T6320 arg1Of had,In
R5269 T6327 T6325 arg1Of had,","
R5270 T6327 T6333 arg1Of had,on
R5271 T6329 T6330 arg1Of rapid,and
R5272 T6331 T6330 arg2Of sustained,and
R5273 T6332 T6327 arg2Of effect,had
R5274 T6332 T6328 arg1Of effect,a
R5275 T6332 T6329 arg1Of effect,rapid
R5276 T6332 T6331 arg1Of effect,sustained
R5277 T6335 T6333 arg2Of production,on
R5278 T6335 T6334 arg1Of production,TNF-α
R5279 T6335 T6337 arg1Of production,vitro
R5280 T6335 T6338 arg1Of production,(
R5281 T6337 T6336 arg1Of vitro,in
R5282 T6339 T6338 arg2Of Figure,(
R5283 T6339 T6340 arg1Of Figure,1
R5284 T6341 T6338 arg3Of ),(
R5571 T6696 T6697 arg1Of PTX,decreases
R5572 T6700 T6697 arg2Of activation,decreases
R5573 T6700 T6698 arg1Of activation,LPS-induced
R5574 T6700 T6699 arg1Of activation,NF-κB
R5575 T6702 T6703 arg1Of phosphorylation,leads
R5576 T6703 T6701 arg2Of leads,Since
R5577 T6703 T6704 arg1Of leads,to
R5578 T6705 T6706 arg1Of ubiquitination,and
R5579 T6706 T6704 arg2Of and,to
R5580 T6706 T6708 arg1Of and,of
R5581 T6707 T6706 arg2Of degradation,and
R5582 T6709 T6710 arg1Of I-κBα,and
R5583 T6711 T6710 arg2Of subsequent,and
R5584 T6713 T6708 arg2Of translocation,of
R5585 T6713 T6709 arg1Of translocation,I-κBα
R5586 T6713 T6711 arg1Of translocation,subsequent
R5587 T6713 T6712 arg1Of translocation,nuclear
R5588 T6713 T6714 arg1Of translocation,of
R5589 T6715 T6714 arg2Of NF-κB,of
R5590 T6717 T6719 arg1Of we,examined
R5591 T6719 T6701 arg1Of examined,Since
R5592 T6719 T6716 arg1Of examined,","
R5593 T6719 T6718 arg1Of examined,first
R5594 T6721 T6719 arg2Of effects,examined
R5595 T6721 T6720 arg1Of effects,the
R5596 T6721 T6722 arg1Of effects,of
R5597 T6721 T6724 arg1Of effects,on
R5598 T6723 T6722 arg2Of PTX,of
R5599 T6725 T6724 arg2Of I-κBα,on
R5600 T6728 T6726 arg1Of phosphorylation,Cytoplasmic
R5601 T6728 T6727 arg1Of phosphorylation,I-κBα
R5602 T6728 T6729 arg1Of phosphorylation,was
R5603 T6728 T6731 arg2Of phosphorylation,increased
R5604 T6728 T6736 arg1Of phosphorylation,compared
R5605 T6731 T6729 arg2Of increased,was
R5606 T6731 T6730 arg1Of increased,markedly
R5607 T6731 T6732 arg1Of increased,following
R5608 T6731 T6735 arg1Of increased,when
R5609 T6734 T6732 arg2Of stimulation,following
R5610 T6734 T6733 arg1Of stimulation,LPS
R5611 T6736 T6735 arg2Of compared,when
R5612 T6736 T6737 arg1Of compared,to
R5613 T6738 T6737 arg2Of control,to
R5614 T6738 T6739 arg1Of control,(
R5615 T6742 T6739 arg2Of 0,(
R5616 T6742 T6740 arg1Of 0,100
R5617 T6742 T6741 arg1Of 0,±
R5618 T6742 T6743 arg1Of 0,vs.
R5619 T6742 T6747 arg1Of 0,;
R5620 T6744 T6745 arg1Of 20,±
R5621 T6745 T6743 arg2Of ±,vs.
R5622 T6746 T6745 arg2Of 18,±
R5623 T6748 T6747 arg2Of P,;
R5624 T6748 T6749 arg1Of P,<
R5625 T6748 T6750 arg1Of P,0.05
R5626 T6751 T6739 arg3Of ),(
R5627 T6753 T6752 arg1Of addition,The
R5628 T6753 T6754 arg1Of addition,of
R5629 T6753 T6757 arg1Of addition,downregulated
R5630 T6755 T6754 arg2Of PTX,of
R5631 T6757 T6756 arg1Of downregulated,significantly
R5632 T6760 T6757 arg2Of phosphorylation,downregulated
R5633 T6760 T6758 arg1Of phosphorylation,LPS-induced
R5634 T6760 T6759 arg1Of phosphorylation,I-κBα
R5635 T6760 T6761 arg1Of phosphorylation,(
R5636 T6762 T6761 arg2Of P,(
R5637 T6762 T6763 arg1Of P,=
R5638 T6762 T6764 arg1Of P,0.02
R5639 T6762 T6765 arg1Of P,;
R5640 T6766 T6765 arg2Of Figure,;
R5641 T6766 T6767 arg1Of Figure,2
R5642 T6768 T6761 arg3Of ),(
R5643 T6772 T6769 arg2Of fashion,In
R5644 T6772 T6770 arg1Of fashion,a
R5645 T6772 T6771 arg1Of fashion,similar
R5646 T6777 T6774 arg1Of phosphorylation,nuclear
R5647 T6777 T6775 arg1Of phosphorylation,NF-κB
R5648 T6777 T6776 arg1Of phosphorylation,p65
R5649 T6777 T6778 arg1Of phosphorylation,was
R5650 T6777 T6779 arg2Of phosphorylation,increased
R5651 T6779 T6769 arg1Of increased,In
R5652 T6779 T6773 arg1Of increased,","
R5653 T6779 T6778 arg2Of increased,was
R5654 T6779 T6780 arg1Of increased,following
R5655 T6782 T6780 arg2Of stimulation,following
R5656 T6782 T6781 arg1Of stimulation,LPS
R5657 T6782 T6783 arg1Of stimulation,(
R5658 T6786 T6783 arg2Of 0,(
R5659 T6786 T6784 arg1Of 0,100
R5660 T6786 T6785 arg1Of 0,±
R5661 T6786 T6787 arg1Of 0,vs.
R5662 T6786 T6791 arg1Of 0,;
R5663 T6788 T6789 arg1Of 38,±
R5664 T6789 T6787 arg2Of ±,vs.
R5665 T6790 T6789 arg2Of 20,±
R5666 T6792 T6791 arg2Of P,;
R5667 T6792 T6793 arg1Of P,<
R5668 T6792 T6794 arg1Of P,0.01
R5669 T6795 T6783 arg3Of ),(
R5670 T6796 T6798 arg1Of PTX,decreased
R5671 T6798 T6797 arg1Of decreased,similarly
R5672 T6803 T6798 arg2Of phosphorylation,decreased
R5673 T6803 T6799 arg1Of phosphorylation,LPS-induced
R5674 T6803 T6800 arg1Of phosphorylation,NF-κB
R5675 T6803 T6801 arg1Of phosphorylation,p65
R5676 T6803 T6802 arg1Of phosphorylation,nuclear
R5677 T6803 T6804 arg1Of phosphorylation,","
R5678 T6806 T6804 arg2Of marker,","
R5679 T6806 T6805 arg1Of marker,a
R5680 T6806 T6807 arg1Of marker,of
R5681 T6809 T6808 arg1Of activation,NF-κB
R5682 T6809 T6810 arg1Of activation,and
R5683 T6810 T6807 arg2Of and,of
R5684 T6812 T6810 arg2Of translocation,and
R5685 T6812 T6811 arg1Of translocation,nuclear
R5686 T6812 T6813 arg1Of translocation,(
R5687 T6816 T6813 arg2Of 0,(
R5688 T6816 T6814 arg1Of 0,100
R5689 T6816 T6815 arg1Of 0,±
R5690 T6816 T6817 arg1Of 0,vs.
R5691 T6816 T6821 arg1Of 0,;
R5692 T6816 T6824 arg1Of 0,;
R5693 T6818 T6819 arg1Of 40,±
R5694 T6819 T6817 arg2Of ±,vs.
R5695 T6820 T6819 arg2Of 6,±
R5696 T6822 T6821 arg2Of P,;
R5697 T6822 T6823 arg1Of P,=0.03
R5698 T6826 T6824 arg2Of 3A,;
R5699 T6826 T6825 arg1Of 3A,Figure
R5700 T6827 T6813 arg3Of ),(
R5701 T6828 T6829 arg1Of EMSAs,were
R5702 T6828 T6831 arg2Of EMSAs,performed
R5703 T6831 T6829 arg2Of performed,were
R5704 T6831 T6830 arg1Of performed,then
R5705 T6833 T6831 arg3Of verify,performed
R5706 T6833 T6832 arg1Of verify,to
R5707 T6836 T6834 arg1Of alterations,the
R5708 T6836 T6835 arg1Of alterations,PTX-induced
R5709 T6836 T6837 arg1Of alterations,in
R5710 T6836 T6840 arg1Of alterations,resulted
R5711 T6839 T6837 arg2Of phosphorylation,in
R5712 T6839 T6838 arg1Of phosphorylation,nuclear
R5713 T6840 T6833 arg2Of resulted,verify
R5714 T6840 T6841 arg1Of resulted,in
R5715 T6840 T6844 arg1Of resulted,on
R5716 T6843 T6841 arg2Of effects,in
R5717 T6843 T6842 arg1Of effects,similar
R5718 T6848 T6844 arg2Of activity,on
R5719 T6848 T6845 arg1Of activity,the
R5720 T6848 T6846 arg1Of activity,DNA
R5721 T6848 T6847 arg1Of activity,binding
R5722 T6848 T6849 arg1Of activity,of
R5723 T6850 T6849 arg2Of NF-κB,of
R5724 T6852 T6851 arg1Of addition,The
R5725 T6852 T6853 arg1Of addition,of
R5726 T6852 T6855 arg1Of addition,to
R5727 T6852 T6859 arg1Of addition,resulted
R5728 T6854 T6853 arg2Of PTX,of
R5729 T6858 T6855 arg2Of cells,to
R5730 T6858 T6856 arg1Of cells,LPS-stimulated
R5731 T6858 T6857 arg1Of cells,mononuclear
R5732 T6859 T6860 arg1Of resulted,in
R5733 T6859 T6867 arg1Of resulted,","
R5734 T6862 T6860 arg2Of downregulation,in
R5735 T6862 T6861 arg1Of downregulation,comparable
R5736 T6862 T6863 arg1Of downregulation,of
R5737 T6866 T6863 arg2Of activity,of
R5738 T6866 T6864 arg1Of activity,DNA
R5739 T6866 T6865 arg1Of activity,binding
R5740 T6868 T6859 arg2Of similar,resulted
R5741 T6868 T6869 arg1Of similar,to
R5742 T6872 T6869 arg2Of downregulation,to
R5743 T6872 T6870 arg1Of downregulation,the
R5744 T6872 T6871 arg2Of downregulation,observed
R5745 T6872 T6873 arg1Of downregulation,of
R5746 T6875 T6873 arg2Of phosphorylation,of
R5747 T6875 T6874 arg1Of phosphorylation,NF-κB
R5748 T6875 T6876 arg1Of phosphorylation,(
R5749 T6878 T6876 arg2Of 3B,(
R5750 T6878 T6877 arg1Of 3B,Figure
R5751 T6879 T6876 arg3Of ),(
R6043 T7258 T7259 arg1Of PTX,upregulates
R6044 T7259 T7264 arg1Of upregulates,after
R6045 T7261 T7262 arg1Of phosphorylation,and
R6046 T7262 T7259 arg2Of and,upregulates
R6047 T7262 T7260 arg1Of and,CREB
R6048 T7263 T7262 arg2Of activation,and
R6049 T7266 T7264 arg2Of stimulation,after
R6050 T7266 T7265 arg1Of stimulation,LPS
R6051 T7267 T7268 arg1Of Phosphorylation,of
R6052 T7267 T7271 arg1Of Phosphorylation,was
R6053 T7267 T7272 arg2Of Phosphorylation,used
R6054 T7270 T7268 arg2Of CREB,of
R6055 T7270 T7269 arg1Of CREB,nuclear
R6056 T7272 T7271 arg2Of used,was
R6057 T7272 T7273 arg1Of used,as
R6058 T7275 T7273 arg2Of marker,as
R6059 T7275 T7274 arg1Of marker,a
R6060 T7275 T7276 arg1Of marker,for
R6061 T7278 T7276 arg2Of activation,for
R6062 T7278 T7277 arg1Of activation,CREB
R6063 T7280 T7279 arg1Of stimulation,LPS
R6064 T7280 T7281 arg1Of stimulation,caused
R6065 T7280 T7289 arg1Of stimulation,compared
R6066 T7281 T7288 arg1Of caused,when
R6067 T7284 T7281 arg2Of increase,caused
R6068 T7284 T7282 arg1Of increase,a
R6069 T7284 T7283 arg1Of increase,negligible
R6070 T7284 T7285 arg1Of increase,in
R6071 T7287 T7285 arg2Of phosphorylation,in
R6072 T7287 T7286 arg1Of phosphorylation,CREB
R6073 T7289 T7288 arg2Of compared,when
R6074 T7289 T7290 arg1Of compared,to
R6075 T7291 T7290 arg2Of control,to
R6076 T7291 T7292 arg1Of control,(
R6077 T7294 T7292 arg2Of 4A,(
R6078 T7294 T7293 arg1Of 4A,Figure
R6079 T7295 T7292 arg3Of ),(
R6080 T7296 T7297 arg1Of PTX,alone
R6081 T7296 T7298 arg1Of PTX,caused
R6082 T7301 T7298 arg2Of increase,caused
R6083 T7301 T7299 arg1Of increase,a
R6084 T7301 T7300 arg1Of increase,marked
R6085 T7301 T7302 arg1Of increase,in
R6086 T7304 T7302 arg2Of phosphorylation,in
R6087 T7304 T7303 arg1Of phosphorylation,CREB
R6088 T7304 T7305 arg1Of phosphorylation,(
R6089 T7306 T7305 arg2Of P,(
R6090 T7306 T7307 arg1Of P,<
R6091 T7306 T7309 arg1Of P,vs.
R6092 T7307 T7308 arg1Of <,0.01
R6093 T7310 T7309 arg2Of HBSS,vs.
R6094 T7311 T7305 arg3Of ),(
R6095 T7313 T7314 arg1Of LPS,and
R6096 T7314 T7317 arg1Of and,occurred
R6097 T7316 T7314 arg2Of exposure,and
R6098 T7316 T7315 arg1Of exposure,PTX
R6099 T7317 T7312 arg2Of occurred,When
R6100 T7317 T7318 arg1Of occurred,simultaneously
R6101 T7321 T7320 arg1Of phosphorylation,CREB
R6102 T7321 T7322 arg1Of phosphorylation,was
R6103 T7321 T7324 arg1Of phosphorylation,higher
R6104 T7322 T7312 arg1Of was,When
R6105 T7322 T7319 arg1Of was,","
R6106 T7322 T7330 arg1Of was,(
R6107 T7324 T7322 arg2Of higher,was
R6108 T7324 T7323 arg1Of higher,significantly
R6109 T7324 T7325 arg1Of higher,than
R6110 T7326 T7325 arg2Of with,than
R6111 T7328 T7326 arg2Of stimulation,with
R6112 T7328 T7327 arg1Of stimulation,LPS
R6113 T7328 T7329 arg1Of stimulation,alone
R6114 T7331 T7332 arg1Of 543,±
R6115 T7332 T7330 arg2Of ±,(
R6116 T7332 T7334 arg1Of ±,vs.
R6117 T7332 T7338 arg1Of ±,;
R6118 T7333 T7332 arg2Of 92,±
R6119 T7337 T7334 arg2Of 0,vs.
R6120 T7337 T7335 arg1Of 0,100
R6121 T7337 T7336 arg1Of 0,±
R6122 T7339 T7338 arg2Of P,;
R6123 T7339 T7340 arg1Of P,<
R6124 T7339 T7341 arg1Of P,0.01
R6125 T7342 T7330 arg3Of ),(
R6126 T7344 T7343 arg2Of addition,In
R6127 T7347 T7346 arg1Of amount,the
R6128 T7347 T7348 arg1Of amount,of
R6129 T7347 T7358 arg1Of amount,was
R6130 T7347 T7359 arg1Of amount,less
R6131 T7350 T7348 arg2Of phosphorylation,of
R6132 T7350 T7349 arg1Of phosphorylation,CREB
R6133 T7350 T7351 arg2Of phosphorylation,seen
R6134 T7351 T7352 arg1Of seen,with
R6135 T7354 T7353 arg1Of LPS,concomitant
R6136 T7354 T7355 arg1Of LPS,and
R6137 T7355 T7352 arg2Of and,with
R6138 T7357 T7355 arg2Of treatment,and
R6139 T7357 T7356 arg1Of treatment,PTX
R6140 T7358 T7343 arg1Of was,In
R6141 T7358 T7345 arg1Of was,","
R6142 T7358 T7366 arg1Of was,","
R6143 T7358 T7367 arg1Of was,although
R6144 T7359 T7358 arg2Of less,was
R6145 T7359 T7360 arg1Of less,than
R6146 T7361 T7360 arg2Of that,than
R6147 T7361 T7362 arg2Of that,seen
R6148 T7362 T7363 arg1Of seen,with
R6149 T7364 T7363 arg2Of PTX,with
R6150 T7364 T7365 arg1Of PTX,alone
R6151 T7369 T7368 arg1Of difference,this
R6152 T7369 T7370 arg1Of difference,was
R6153 T7369 T7373 arg1Of difference,significant
R6154 T7370 T7367 arg2Of was,although
R6155 T7370 T7371 arg1Of was,not
R6156 T7370 T7372 arg1Of was,statistically
R6157 T7373 T7370 arg2Of significant,was
R6158 T7373 T7374 arg1Of significant,(
R6159 T7375 T7374 arg2Of P,(
R6160 T7375 T7376 arg1Of P,=
R6161 T7375 T7377 arg1Of P,0.08
R6162 T7378 T7374 arg3Of ),(
R6163 T7380 T7379 arg1Of determine,To
R6164 T7383 T7382 arg1Of binding,CREB-DNA
R6165 T7383 T7384 arg1Of binding,was
R6166 T7383 T7385 arg2Of binding,affected
R6167 T7385 T7380 arg2Of affected,determine
R6168 T7385 T7381 arg1Of affected,if
R6169 T7385 T7384 arg2Of affected,was
R6170 T7385 T7388 arg1Of affected,in
R6171 T7387 T7385 arg1Of PTX,affected
R6172 T7387 T7386 arg2Of PTX,by
R6173 T7390 T7388 arg2Of manner,in
R6174 T7390 T7389 arg1Of manner,a
R6175 T7390 T7391 arg1Of manner,similar
R6176 T7391 T7392 arg1Of similar,to
R6177 T7394 T7392 arg2Of phosphorylation,to
R6178 T7394 T7393 arg1Of phosphorylation,CREB
R6179 T7397 T7396 arg1Of EMSA,an
R6180 T7397 T7398 arg1Of EMSA,was
R6181 T7397 T7399 arg2Of EMSA,performed
R6182 T7399 T7379 modOf performed,To
R6183 T7399 T7395 arg1Of performed,","
R6184 T7399 T7398 arg2Of performed,was
R6185 T7400 T7401 arg1Of Exposure,of
R6186 T7400 T7404 arg1Of Exposure,to
R6187 T7400 T7407 arg1Of Exposure,increased
R6188 T7403 T7401 arg2Of cells,of
R6189 T7403 T7402 arg1Of cells,LPS-stimulated
R6190 T7405 T7404 arg2Of PTX,to
R6191 T7407 T7406 arg1Of increased,similarly
R6192 T7407 T7410 arg1Of increased,compared
R6193 T7409 T7407 arg2Of DNA-binding,increased
R6194 T7409 T7408 arg1Of DNA-binding,CREB
R6195 T7411 T7410 arg2Of to,compared
R6196 T7412 T7411 arg2Of LPS,to
R6197 T7412 T7413 arg1Of LPS,alone
R6198 T7412 T7414 arg1Of LPS,(
R6199 T7416 T7414 arg2Of 4B,(
R6200 T7416 T7415 arg1Of 4B,Figure
R6201 T7417 T7414 arg3Of ),(
R6417 T7755 T7756 arg1Of Effect,of
R6418 T7755 T7758 arg1Of Effect,on
R6419 T7757 T7756 arg2Of PTX,of
R6420 T7760 T7758 arg2Of production,on
R6421 T7760 T7759 arg1Of production,TNF-α
R6422 T7760 T7761 arg1Of production,in
R6423 T7763 T7761 arg2Of presence,in
R6424 T7763 T7762 arg1Of presence,the
R6425 T7763 T7764 arg1Of presence,of
R6426 T7766 T7764 arg2Of inhibition,of
R6427 T7766 T7765 arg1Of inhibition,PKA
R6428 T7768 T7767 arg1Of role,The
R6429 T7768 T7769 arg1Of role,of
R6430 T7768 T7771 arg1Of role,in
R6431 T7768 T7777 arg1Of role,in
R6432 T7768 T7781 arg1Of role,was
R6433 T7768 T7782 arg2Of role,assessed
R6434 T7770 T7769 arg2Of PKA,of
R6435 T7773 T7771 arg2Of downregulation,in
R6436 T7773 T7772 arg1Of downregulation,PTX-induced
R6437 T7773 T7774 arg1Of downregulation,of
R6438 T7776 T7774 arg2Of production,of
R6439 T7776 T7775 arg1Of production,TNF-α
R6440 T7780 T7777 arg2Of cells,in
R6441 T7780 T7778 arg1Of cells,LPS-stimulated
R6442 T7780 T7779 arg1Of cells,mononuclear
R6443 T7782 T7781 arg2Of assessed,was
R6444 T7782 T7783 arg1Of assessed,by
R6445 T7784 T7783 arg2Of treating,by
R6446 T7786 T7784 arg2Of cells,treating
R6447 T7786 T7785 arg1Of cells,mononuclear
R6448 T7786 T7787 arg1Of cells,with
R6449 T7786 T7793 arg1Of cells,","
R6450 T7786 T7794 arg1Of cells,prior
R6451 T7788 T7787 arg2Of H89,with
R6452 T7788 T7789 arg1Of H89,","
R6453 T7792 T7789 arg2Of inhibitor,","
R6454 T7792 T7790 arg1Of inhibitor,a
R6455 T7792 T7791 arg1Of inhibitor,PKA
R6456 T7794 T7795 arg1Of prior,to
R6457 T7796 T7797 arg1Of LPS,and
R6458 T7797 T7795 arg2Of and,to
R6459 T7799 T7797 arg2Of exposure,and
R6460 T7799 T7798 arg1Of exposure,PTX
R6461 T7802 T7800 arg1Of PTX,The
R6462 T7802 T7801 arg1Of PTX,addition
R6463 T7802 T7803 arg1Of PTX,to
R6464 T7802 T7806 arg1Of PTX,resulted
R6465 T7805 T7803 arg2Of cells,to
R6466 T7805 T7804 arg1Of cells,LPS-stimulated
R6467 T7806 T7807 arg1Of resulted,in
R6468 T7809 T7807 arg2Of reduction,in
R6469 T7809 T7808 arg1Of reduction,a
R6470 T7809 T7810 arg1Of reduction,in
R6471 T7809 T7812 arg1Of reduction,","
R6472 T7809 T7813 arg1Of reduction,similar
R6473 T7809 T7821 arg1Of reduction,(
R6474 T7811 T7810 arg2Of TNF-α,in
R6475 T7813 T7814 arg1Of similar,to
R6476 T7815 T7814 arg2Of that,to
R6477 T7815 T7816 arg2Of that,observed
R6478 T7816 T7817 arg1Of observed,in
R6479 T7820 T7817 arg2Of experiments,in
R6480 T7820 T7818 arg1Of experiments,our
R6481 T7820 T7819 arg1Of experiments,previous
R6482 T7823 T7822 arg1Of pg/mL,529
R6483 T7824 T7823 arg1Of ±,pg/mL
R6484 T7827 T7826 arg1Of 37,vs.
R6485 T7829 T7821 arg2Of ±,(
R6486 T7829 T7824 arg1Of ±,±
R6487 T7829 T7825 arg1Of ±,112
R6488 T7829 T7827 arg1Of ±,37
R6489 T7829 T7828 arg1Of ±,pg/mL
R6490 T7829 T7830 arg1Of ±,10
R6491 T7829 T7831 arg1Of ±,;
R6492 T7832 T7831 arg2Of P,;
R6493 T7832 T7833 arg1Of P,<
R6494 T7832 T7834 arg1Of P,0.01
R6495 T7835 T7821 arg3Of ),(
R6496 T7836 T7837 arg1Of Pre-incubation,of
R6497 T7836 T7839 arg1Of Pre-incubation,with
R6498 T7836 T7847 arg1Of Pre-incubation,did
R6499 T7836 T7849 arg1Of Pre-incubation,result
R6500 T7838 T7837 arg2Of cells,of
R6501 T7840 T7839 arg2Of H89,with
R6502 T7840 T7841 arg1Of H89,prior
R6503 T7841 T7842 arg1Of prior,to
R6504 T7843 T7844 arg1Of LPS,and
R6505 T7845 T7844 arg2Of PTX,and
R6506 T7846 T7842 arg2Of exposure,to
R6507 T7846 T7843 arg1Of exposure,LPS
R6508 T7846 T7845 arg1Of exposure,PTX
R6509 T7849 T7847 arg2Of result,did
R6510 T7849 T7848 arg1Of result,not
R6511 T7849 T7850 arg1Of result,in
R6512 T7853 T7850 arg2Of modulation,in
R6513 T7853 T7851 arg1Of modulation,any
R6514 T7853 T7852 arg1Of modulation,significant
R6515 T7853 T7854 arg1Of modulation,of
R6516 T7853 T7858 arg1Of modulation,compared
R6517 T7853 T7861 arg2Of modulation,demonstrated
R6518 T7856 T7854 arg2Of production,of
R6519 T7856 T7855 arg1Of production,TNF-α
R6520 T7858 T7857 arg2Of compared,when
R6521 T7858 T7859 arg1Of compared,to
R6522 T7860 T7859 arg2Of that,to
R6523 T7861 T7857 arg1Of demonstrated,when
R6524 T7861 T7862 arg1Of demonstrated,with
R6525 T7863 T7862 arg2Of PTX,with
R6526 T7863 T7864 arg1Of PTX,alone
R6527 T7864 T7865 arg1Of alone,(
R6528 T7866 T7865 arg2Of P,(
R6529 T7866 T7867 arg1Of P,=
R6530 T7866 T7868 arg1Of P,0.2
R6531 T7866 T7869 arg1Of P,;
R6532 T7870 T7869 arg2Of Figure,;
R6533 T7870 T7871 arg1Of Figure,5
R6534 T7872 T7865 arg3Of ),(
R6535 T7874 T7873 arg1Of results,These
R6536 T7874 T7875 arg1Of results,suggest
R6537 T7877 T7878 arg1Of downregulation,of
R6538 T7877 T7883 arg1Of downregulation,in
R6539 T7877 T7887 arg1Of downregulation,is
R6540 T7880 T7878 arg2Of production,of
R6541 T7880 T7879 arg1Of production,TNF-α
R6542 T7880 T7881 arg1Of production,by
R6543 T7882 T7881 arg2Of PTX,by
R6544 T7886 T7883 arg2Of cells,in
R6545 T7886 T7884 arg1Of cells,LPS-stimulated
R6546 T7886 T7885 arg1Of cells,mononuclear
R6547 T7887 T7875 arg2Of is,suggest
R6548 T7887 T7876 arg1Of is,that
R6549 T7887 T7888 arg1Of is,","
R6550 T7887 T7891 arg1Of is,in
R6551 T7887 T7893 arg1Of is,","
R6552 T7890 T7889 arg1Of least,at
R6553 T7891 T7890 arg1Of in,least
R6554 T7892 T7891 arg2Of part,in
R6555 T7894 T7887 arg2Of PKA-independent,is
R3312 T4034 T4035 arg1Of concentration,of
R3313 T4034 T4037 arg1Of concentration,was
R3314 T4034 T4039 arg2Of concentration,determined
R3315 T4036 T4035 arg2Of TNF-α,of
R3316 T4039 T4037 arg2Of determined,was
R3317 T4039 T4038 arg1Of determined,quantitatively
R3318 T4041 T4039 arg1Of ELISA,determined
R3319 T4041 T4040 arg2Of ELISA,by
R3320 T4041 T4042 arg1Of ELISA,in
R3321 T4044 T4042 arg2Of media,in
R3322 T4044 T4043 arg1Of media,the

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T162 244-254 NN denotes inhibition
T163 255-257 IN denotes on
T164 258-271 NN denotes transcription
T165 272-278 NN denotes factor
T166 279-289 NN denotes activation
T167 290-293 CC denotes and
T168 294-299 NN denotes tumor
T169 300-308 NN denotes necrosis
T170 309-321 NN denotes factor-alpha
T171 322-323 -LRB- denotes (
T172 323-328 NNP denotes TNF-α
T173 328-329 -RRB- denotes )
T174 330-340 NN denotes production
T175 341-343 IN denotes in
T176 344-362 NN denotes lipopolysaccharide
T177 363-364 -LRB- denotes (
T178 364-367 NNP denotes LPS
T179 367-368 -RRB- denotes )
T180 368-369 : denotes -
T181 369-379 VBN denotes stimulated
T182 380-385 JJ denotes human
T183 386-397 NN denotes mononuclear
T184 398-403 NNS denotes cells
T185 403-404 . denotes .
T186 406-418 NNP denotes INTRODUCTION
T187 419-422 DT denotes The
T188 423-433 NN denotes production
T189 434-436 IN denotes of
T190 437-442 NNP denotes TNF-α
T191 443-452 VBG denotes following
T192 453-456 NNP denotes LPS
T193 457-468 NN denotes stimulation
T194 469-471 VBZ denotes is
T195 472-475 CD denotes one
T196 476-478 IN denotes of
T197 479-482 DT denotes the
T198 483-486 JJ denotes key
T199 487-492 NNS denotes steps
T200 493-495 IN denotes in
T201 496-505 JJ denotes bacterial
T202 506-512 NN denotes sepsis
T203 513-516 CC denotes and
T204 517-529 NN denotes inflammation
T205 529-530 . denotes .
T206 531-534 DT denotes The
T207 535-544 NN denotes mechanism
T208 545-547 IN denotes by
T209 548-553 WDT denotes which
T210 554-571 NN denotes phosphodiesterase
T211 572-582 NN denotes inhibition
T212 583-589 VBZ denotes alters
T213 590-595 JJ denotes TNF-α
T214 596-606 NN denotes production
T215 607-609 IN denotes in
T216 610-613 DT denotes the
T217 614-622 NN denotes presence
T218 623-625 IN denotes of
T219 626-629 NNP denotes LPS
T220 630-637 VBZ denotes remains
T221 638-645 JJ denotes unclear
T222 645-646 . denotes .
T223 648-655 NNP denotes METHODS
T224 656-661 NNP denotes Human
T225 662-673 NN denotes mononuclear
T226 674-679 NNS denotes cells
T227 680-684 VBD denotes were
T228 685-695 VBN denotes stimulated
T229 696-700 IN denotes with
T230 701-704 NNP denotes LPS
T231 705-706 -LRB- denotes (
T232 706-707 CD denotes 1
T233 708-710 NN denotes μg
T234 710-711 NN denotes /
T235 711-713 NNP denotes mL
T236 713-714 -RRB- denotes )
T237 714-715 , denotes ,
T238 716-718 IN denotes in
T239 719-722 DT denotes the
T240 723-731 NN denotes presence
T241 732-735 CC denotes and
T242 736-743 NN denotes absence
T243 744-746 IN denotes of
T244 747-761 NNP denotes Pentoxifylline
T245 762-763 -LRB- denotes (
T246 763-766 NNP denotes PTX
T247 766-767 : denotes ;
T248 768-770 CD denotes 20
T249 771-773 NNP denotes mM
T250 773-774 -RRB- denotes )
T251 774-775 , denotes ,
T252 776-777 DT denotes a
T253 778-789 JJ denotes nonspecific
T254 790-807 NN denotes phosphodiesterase
T255 808-817 NN denotes inhibitor
T256 817-818 . denotes .
T257 819-826 JJ denotes Western
T258 827-835 VBG denotes blotting
T259 836-838 IN denotes of
T260 839-853 VBN denotes phosphorylated
T261 854-865 JJ denotes cytoplasmic
T262 866-871 NNP denotes I-κBα
T263 871-872 , denotes ,
T264 873-880 JJ denotes nuclear
T265 881-890 NNP denotes factor-κB
T266 891-894 NNS denotes p65
T267 895-896 -LRB- denotes (
T268 896-901 NN denotes NF-κB
T269 901-902 -RRB- denotes )
T270 902-903 , denotes ,
T271 904-907 CC denotes and
T272 908-915 JJ denotes nuclear
T273 916-929 JJ denotes cAMP-response
T274 930-937 NN denotes element
T275 938-945 JJ denotes binding
T276 946-953 NN denotes protein
T277 954-955 -LRB- denotes (
T278 955-959 NNP denotes CREB
T279 959-960 -RRB- denotes )
T280 961-964 VBD denotes was
T281 965-974 VBN denotes performed
T282 974-975 . denotes .
T283 976-979 NNP denotes DNA
T284 980-987 JJ denotes binding
T285 988-990 IN denotes of
T286 991-996 NNP denotes NF-κB
T287 997-1000 CC denotes and
T288 1001-1005 NNP denotes CREB
T289 1006-1009 VBD denotes was
T290 1010-1018 VBN denotes verified
T291 1019-1021 IN denotes by
T292 1022-1037 JJ denotes electrophoretic
T293 1038-1046 NN denotes mobility
T294 1047-1052 NN denotes shift
T295 1053-1058 NN denotes assay
T296 1058-1059 . denotes .
T297 1060-1065 JJ denotes TNF-α
T298 1066-1072 NNS denotes levels
T299 1073-1077 VBD denotes were
T300 1078-1088 VBN denotes determined
T301 1089-1091 IN denotes in
T302 1092-1095 DT denotes the
T303 1096-1107 NN denotes supernatant
T304 1108-1110 IN denotes of
T305 1111-1121 VBN denotes stimulated
T306 1122-1127 NNS denotes cells
T307 1128-1130 IN denotes in
T308 1131-1134 DT denotes the
T309 1135-1143 NN denotes presence
T310 1144-1147 CC denotes and
T311 1148-1155 NN denotes absence
T312 1156-1163 NNP denotes Protein
T313 1164-1170 NN denotes kinase
T314 1171-1172 DT denotes A
T315 1173-1183 NN denotes inhibition
T316 1184-1186 IN denotes by
T317 1187-1189 DT denotes an
T318 1190-1203 JJ denotes enzyme-linked
T319 1204-1217 JJ denotes immunosorbent
T320 1218-1223 NN denotes assay
T321 1224-1225 -LRB- denotes (
T322 1225-1230 NNP denotes ELISA
T323 1230-1231 -RRB- denotes )
T324 1231-1232 . denotes .
T325 1234-1241 NNS denotes RESULTS
T326 1242-1245 VBP denotes PTX
T327 1246-1249 VBD denotes was
T328 1250-1262 VBN denotes demonstrated
T329 1263-1265 TO denotes to
T330 1266-1279 RB denotes significantly
T331 1280-1286 VB denotes reduce
T332 1287-1298 JJ denotes cytoplasmic
T333 1299-1304 JJ denotes I-κBα
T334 1305-1320 NN denotes phosphorylation
T335 1320-1321 , denotes ,
T336 1322-1329 JJ denotes nuclear
T337 1330-1333 NNS denotes p65
T338 1334-1349 NN denotes phosphorylation
T339 1349-1350 , denotes ,
T340 1351-1354 CC denotes and
T341 1355-1358 DT denotes the
T342 1359-1362 NNP denotes DNA
T343 1363-1370 JJ denotes binding
T344 1371-1379 NN denotes activity
T345 1380-1382 IN denotes of
T346 1383-1388 NN denotes NF-κB
T347 1388-1389 . denotes .
T348 1390-1392 IN denotes In
T349 1393-1401 NN denotes contrast
T350 1401-1402 , denotes ,
T351 1403-1406 NNP denotes PTX
T352 1407-1415 RB denotes markedly
T353 1416-1424 VBD denotes enhanced
T354 1425-1428 DT denotes the
T355 1429-1444 NN denotes phosphorylation
T356 1445-1448 CC denotes and
T357 1449-1452 NN denotes DNA
T358 1453-1460 JJ denotes binding
T359 1461-1469 NN denotes activity
T360 1470-1472 IN denotes of
T361 1473-1477 NNP denotes CREB
T362 1477-1478 . denotes .
T363 1479-1484 NNS denotes Cells
T364 1485-1498 RB denotes concomitantly
T365 1499-1506 VBN denotes treated
T366 1507-1511 IN denotes with
T367 1512-1515 NNP denotes PTX
T368 1516-1519 CC denotes and
T369 1520-1523 NNP denotes LPS
T370 1524-1532 VBD denotes secreted
T371 1533-1540 JJ denotes similar
T372 1541-1547 NNS denotes levels
T373 1548-1550 IN denotes of
T374 1551-1556 NNP denotes TNF-α
T375 1557-1559 IN denotes in
T376 1560-1563 DT denotes the
T377 1564-1572 NN denotes presence
T378 1573-1576 CC denotes and
T379 1577-1584 NN denotes absence
T380 1585-1592 NNP denotes Protein
T381 1593-1599 NN denotes kinase
T382 1600-1601 DT denotes A
T383 1602-1612 NN denotes inhibition
T384 1612-1613 . denotes .
T385 1615-1625 NNP denotes DISCUSSION
T386 1626-1629 NNP denotes The
T387 1630-1639 VBD denotes increased
T388 1640-1645 NN denotes level
T389 1646-1648 IN denotes of
T390 1649-1653 NNP denotes cAMP
T391 1654-1658 WDT denotes that
T392 1659-1666 VBZ denotes results
T393 1667-1671 IN denotes from
T394 1672-1689 NN denotes phosphodiesterase
T395 1690-1700 NN denotes inhibition
T396 1701-1708 VBZ denotes affects
T397 1709-1720 JJ denotes cytoplasmic
T398 1721-1724 CC denotes and
T399 1725-1732 JJ denotes nuclear
T400 1733-1739 NNS denotes events
T401 1739-1740 , denotes ,
T402 1741-1750 VBG denotes resulting
T403 1751-1753 IN denotes in
T404 1754-1757 DT denotes the
T405 1758-1769 NN denotes attenuation
T406 1770-1772 IN denotes of
T407 1773-1778 NNP denotes NF-κB
T408 1779-1782 CC denotes and
T409 1783-1786 DT denotes the
T410 1787-1797 NN denotes activation
T411 1798-1800 IN denotes of
T412 1801-1805 NNP denotes CREB
T413 1806-1821 JJ denotes transcriptional
T414 1822-1825 NNP denotes DNA
T415 1826-1833 JJ denotes binding
T416 1834-1841 IN denotes through
T417 1842-1850 NNS denotes pathways
T418 1851-1855 WDT denotes that
T419 1856-1859 VBP denotes are
T420 1860-1869 RB denotes partially
T421 1870-1877 NNP denotes Protein
T422 1878-1884 NN denotes kinase
T423 1885-1898 NN denotes A-independent
T424 1898-1899 . denotes .
T425 1901-1911 NNP denotes CONCLUSION
T426 1912-1924 JJ denotes PTX-mediated
T427 1925-1942 NN denotes phosphodiesterase
T428 1943-1953 NN denotes inhibition
T429 1954-1960 VBZ denotes occurs
T430 1961-1970 RB denotes partially
T431 1971-1978 IN denotes through
T432 1979-1980 DT denotes a
T433 1981-1988 NNP denotes Protein
T434 1989-1995 NN denotes kinase
T435 1996-2009 JJ denotes A-independent
T436 2010-2017 NN denotes pathway
T437 2018-2021 CC denotes and
T438 2022-2025 MD denotes may
T439 2026-2031 VB denotes serve
T440 2032-2034 IN denotes as
T441 2035-2036 DT denotes a
T442 2037-2043 JJ denotes useful
T443 2044-2048 NN denotes tool
T444 2049-2051 IN denotes in
T445 2052-2055 DT denotes the
T446 2056-2067 NN denotes attenuation
T447 2068-2070 IN denotes of
T448 2071-2082 JJ denotes LPS-induced
T449 2083-2095 NN denotes inflammation
T450 2095-2096 . denotes .
T1162 2112-2115 DT denotes The
T1163 2116-2125 JJ denotes bacterial
T1164 2126-2134 NN denotes membrane
T1165 2135-2144 NN denotes component
T1166 2145-2163 NN denotes lipopolysaccharide
T1167 2164-2165 -LRB- denotes (
T1168 2165-2168 NNP denotes LPS
T1169 2168-2169 -RRB- denotes )
T1170 2170-2172 VBZ denotes is
T1171 2173-2180 JJ denotes capable
T1172 2181-2183 IN denotes of
T1173 2184-2194 VBG denotes initiating
T1174 2195-2210 NN denotes phosphorylation
T1175 2211-2214 CC denotes and
T1176 2215-2225 NN denotes activation
T1177 2226-2228 IN denotes of
T1178 2229-2237 JJ denotes multiple
T1179 2238-2242 NN denotes host
T1180 2243-2256 JJ denotes intracellular
T1181 2257-2264 NN denotes protein
T1182 2265-2272 NNS denotes kinases
T1183 2273-2276 CC denotes and
T1184 2277-2290 NN denotes transcription
T1185 2291-2298 NNS denotes factors
T1186 2298-2299 . denotes .
T1187 2300-2313 NNP denotes Transcription
T1188 2314-2320 NN denotes factor
T1189 2321-2331 NN denotes activation
T1190 2331-2332 , denotes ,
T1191 2333-2338 WDT denotes which
T1192 2339-2346 VBZ denotes results
T1193 2347-2349 IN denotes in
T1194 2350-2353 DT denotes the
T1195 2354-2364 NN denotes modulation
T1196 2365-2367 IN denotes of
T1197 2368-2372 NN denotes gene
T1198 2373-2386 NN denotes transcription
T1199 2387-2390 CC denotes and
T1200 2391-2398 NN denotes protein
T1201 2399-2408 NN denotes synthesis
T1202 2408-2409 , denotes ,
T1203 2410-2412 VBZ denotes is
T1204 2413-2414 DT denotes a
T1205 2415-2423 JJ denotes critical
T1206 2424-2431 NN denotes element
T1207 2432-2434 IN denotes in
T1208 2435-2438 DT denotes the
T1209 2439-2446 NN denotes defense
T1210 2447-2456 NN denotes mechanism
T1211 2457-2459 IN denotes of
T1212 2460-2463 DT denotes the
T1213 2464-2468 NN denotes host
T1214 2469-2475 JJ denotes immune
T1215 2476-2482 NN denotes system
T1216 2482-2486 CD denotes .1,2
T1217 2487-2498 JJ denotes LPS-induced
T1218 2499-2512 NN denotes transcription
T1219 2513-2519 NN denotes factor
T1220 2520-2530 NN denotes activation
T1221 2531-2534 VBZ denotes has
T1222 2535-2539 VBN denotes been
T1223 2540-2545 VBN denotes shown
T1224 2546-2548 TO denotes to
T1225 2549-2551 VB denotes be
T1226 2552-2553 DT denotes a
T1227 2554-2557 JJ denotes key
T1228 2558-2567 NN denotes regulator
T1229 2568-2570 IN denotes of
T1230 2571-2576 NN denotes tumor
T1231 2577-2585 NN denotes necrosis
T1232 2586-2594 NN denotes factor-α
T1233 2595-2596 -LRB- denotes (
T1234 2596-2601 NNP denotes TNF-α
T1235 2601-2602 -RRB- denotes )
T1236 2603-2613 NN denotes production
T1237 2613-2615 CD denotes .3
T1238 2616-2621 NNP denotes TNF-α
T1239 2622-2624 VBZ denotes is
T1240 2625-2626 DT denotes a
T1241 2627-2633 JJ denotes potent
T1242 2634-2650 JJ denotes pro-inflammatory
T1243 2651-2659 NN denotes cytokine
T1244 2660-2668 VBN denotes involved
T1245 2669-2671 IN denotes in
T1246 2672-2673 DT denotes a
T1247 2674-2678 JJ denotes wide
T1248 2679-2687 NN denotes spectrum
T1249 2688-2690 IN denotes of
T1250 2691-2699 JJ denotes cellular
T1251 2700-2709 NNS denotes responses
T1252 2709-2710 . denotes .
T1253 2711-2722 RB denotes Furthermore
T1254 2722-2723 , denotes ,
T1255 2724-2729 JJ denotes TNF-α
T1256 2730-2739 NN denotes synthesis
T1257 2740-2743 MD denotes can
T1258 2744-2746 VB denotes be
T1259 2747-2757 VBN denotes attenuated
T1260 2758-2760 IN denotes in
T1261 2761-2767 JJ denotes immune
T1262 2768-2773 NNS denotes cells
T1263 2774-2781 VBN denotes exposed
T1264 2782-2784 TO denotes to
T1265 2785-2802 VB denotes phosphodiesterase
T1266 2803-2804 -LRB- denotes (
T1267 2804-2807 NNP denotes PDE
T1268 2807-2808 -RRB- denotes )
T1269 2809-2819 NN denotes inhibition
T1270 2820-2825 IN denotes after
T1271 2826-2835 NN denotes challenge
T1272 2836-2838 IN denotes by
T1273 2839-2840 DT denotes a
T1274 2841-2848 NN denotes variety
T1275 2849-2851 IN denotes of
T1276 2852-2868 JJ denotes pro-inflammatory
T1277 2869-2879 NNS denotes stimulants
T1278 2879-2881 CD denotes .4
T1279 2882-2885 DT denotes The
T1280 2886-2895 VBG denotes signaling
T1281 2896-2906 NNS denotes mechanisms
T1282 2907-2915 VBN denotes affected
T1283 2916-2918 IN denotes by
T1284 2919-2922 NNP denotes PDE
T1285 2923-2933 NN denotes inhibition
T1286 2933-2934 , denotes ,
T1287 2935-2940 WDT denotes which
T1288 2941-2951 RB denotes ultimately
T1289 2952-2956 VBP denotes lead
T1290 2957-2959 TO denotes to
T1291 2960-2963 DT denotes the
T1292 2964-2978 NN denotes downregulation
T1293 2979-2981 IN denotes of
T1294 2982-2987 JJ denotes TNF-α
T1295 2988-2998 NN denotes production
T1296 2998-2999 , denotes ,
T1297 3000-3004 VBP denotes have
T1298 3005-3008 RB denotes not
T1299 3009-3013 VBN denotes been
T1300 3014-3018 RB denotes well
T1301 3019-3032 VBN denotes characterized
T1302 3033-3035 IN denotes in
T1303 3036-3048 JJ denotes inflammatory
T1304 3049-3054 NNS denotes cells
T1305 3054-3055 . denotes .
T1306 3056-3067 NNP denotes Classically
T1307 3067-3068 , denotes ,
T1308 3069-3071 PRP denotes it
T1309 3072-3075 VBZ denotes has
T1310 3076-3080 VBN denotes been
T1311 3081-3093 VBN denotes demonstrated
T1312 3094-3098 IN denotes that
T1313 3099-3102 NNP denotes PDE
T1314 3103-3113 NN denotes inhibition
T1315 3114-3121 NNS denotes results
T1316 3122-3124 IN denotes in
T1317 3125-3128 DT denotes the
T1318 3129-3142 JJ denotes intracellular
T1319 3143-3155 NN denotes accumulation
T1320 3156-3158 IN denotes of
T1321 3159-3162 DT denotes the
T1322 3163-3169 JJ denotes second
T1323 3170-3179 NN denotes messenger
T1324 3180-3186 JJ denotes cyclic
T1325 3187-3198 JJ denotes adenosine-3
T1326 3198-3200 SYM denotes ,5
T1327 3200-3201 : denotes -
T1328 3201-3214 NN denotes monophosphate
T1329 3215-3216 -LRB- denotes (
T1330 3216-3220 NNP denotes cAMP
T1331 3220-3221 -RRB- denotes )
T1332 3222-3225 CC denotes and
T1333 3226-3236 JJ denotes subsequent
T1334 3237-3247 NN denotes activation
T1335 3248-3250 IN denotes of
T1336 3251-3258 NNP denotes Protein
T1337 3259-3265 VBP denotes kinase
T1338 3266-3267 DT denotes A
T1339 3268-3269 -LRB- denotes (
T1340 3269-3272 NNP denotes PKA
T1341 3272-3273 -RRB- denotes )
T1342 3273-3275 CD denotes .5
T1343 3276-3279 NNP denotes PKA
T1344 3280-3290 NN denotes activation
T1345 3291-3295 RB denotes then
T1346 3296-3301 VBZ denotes leads
T1347 3302-3304 TO denotes to
T1348 3305-3308 DT denotes the
T1349 3309-3324 NN denotes phosphorylation
T1350 3325-3327 IN denotes of
T1351 3328-3331 DT denotes the
T1352 3332-3345 NN denotes transcription
T1353 3346-3352 NN denotes factor
T1354 3353-3366 JJ denotes cAMP-response
T1355 3367-3374 NN denotes element
T1356 3375-3382 JJ denotes binding
T1357 3383-3390 NN denotes protein
T1358 3391-3392 -LRB- denotes (
T1359 3392-3396 NNP denotes CREB
T1360 3396-3397 -RRB- denotes )
T1361 3397-3398 , denotes ,
T1362 3399-3411 NN denotes transmission
T1363 3412-3414 IN denotes of
T1364 3415-3422 NNS denotes signals
T1365 3423-3427 IN denotes into
T1366 3428-3431 DT denotes the
T1367 3432-3439 NN denotes nucleus
T1368 3439-3440 , denotes ,
T1369 3441-3444 CC denotes and
T1370 3445-3448 DT denotes the
T1371 3449-3459 JJ denotes subsequent
T1372 3460-3470 NN denotes modulation
T1373 3471-3473 IN denotes of
T1374 3474-3478 NN denotes gene
T1375 3479-3492 NN denotes transcription
T1376 3492-3494 CD denotes .6
T1377 3495-3499 DT denotes This
T1378 3500-3510 RB denotes apparently
T1379 3511-3517 JJ denotes simple
T1380 3518-3524 JJ denotes linear
T1381 3525-3532 NN denotes cascade
T1382 3533-3537 VBZ denotes does
T1383 3538-3541 RB denotes not
T1384 3542-3547 RB denotes fully
T1385 3548-3555 VB denotes explain
T1386 3556-3559 DT denotes the
T1387 3560-3569 NN denotes mechanism
T1388 3570-3572 IN denotes by
T1389 3573-3578 WDT denotes which
T1390 3579-3581 DT denotes an
T1391 3582-3591 NN denotes elevation
T1392 3592-3594 IN denotes in
T1393 3595-3598 DT denotes the
T1394 3599-3612 JJ denotes intracellular
T1395 3613-3617 NNP denotes cAMP
T1396 3618-3623 NN denotes level
T1397 3624-3630 VBZ denotes exerts
T1398 3631-3643 JJ denotes wide-ranging
T1399 3644-3651 NNS denotes effects
T1400 3652-3654 IN denotes on
T1401 3655-3663 JJ denotes multiple
T1402 3664-3672 JJ denotes cellular
T1403 3673-3682 NNS denotes functions
T1404 3682-3683 . denotes .
T1405 3684-3689 EX denotes There
T1406 3690-3692 VBZ denotes is
T1407 3693-3694 DT denotes a
T1408 3695-3702 VBG denotes growing
T1409 3703-3707 NN denotes body
T1410 3708-3710 IN denotes of
T1411 3711-3719 NN denotes evidence
T1412 3720-3730 VBG denotes suggesting
T1413 3731-3735 IN denotes that
T1414 3736-3740 NNP denotes cAMP
T1415 3741-3744 MD denotes may
T1416 3745-3753 VB denotes function
T1417 3754-3761 IN denotes through
T1418 3762-3766 DT denotes both
T1419 3767-3780 JJ denotes PKA-dependent
T1420 3781-3784 CC denotes and
T1421 3785-3786 : denotes -
T1422 3786-3797 JJ denotes independent
T1423 3798-3808 NNS denotes mechanisms
T1424 3808-3810 CD denotes .6
T1425 3811-3812 CD denotes 8
T1426 3813-3824 JJ denotes LPS-induced
T1427 3825-3835 NN denotes activation
T1428 3836-3838 IN denotes of
T1429 3839-3842 DT denotes the
T1430 3843-3856 NN denotes transcription
T1431 3857-3863 NN denotes factor
T1432 3864-3871 JJ denotes nuclear
T1433 3872-3881 NNP denotes factor-κB
T1434 3882-3883 -LRB- denotes (
T1435 3883-3888 NNP denotes NF-κB
T1436 3888-3889 -RRB- denotes )
T1437 3890-3893 VBZ denotes has
T1438 3894-3898 RB denotes also
T1439 3899-3903 VBN denotes been
T1440 3904-3907 DT denotes the
T1441 3908-3913 NN denotes focus
T1442 3914-3916 IN denotes of
T1443 3917-3918 DT denotes a
T1444 3919-3924 JJ denotes great
T1445 3925-3929 NN denotes deal
T1446 3930-3932 IN denotes of
T1447 3933-3941 NN denotes research
T1448 3941-3942 . denotes .
T1449 3943-3945 PRP denotes It
T1450 3946-3949 VBZ denotes has
T1451 3950-3954 VBN denotes been
T1452 3955-3962 RB denotes clearly
T1453 3963-3975 VBN denotes demonstrated
T1454 3976-3980 IN denotes that
T1455 3981-3987 NNS denotes agents
T1456 3988-3992 WDT denotes that
T1457 3993-4001 VBP denotes increase
T1458 4002-4015 JJ denotes intracellular
T1459 4016-4020 NNP denotes cAMP
T1460 4021-4025 RB denotes also
T1461 4026-4033 VBP denotes inhibit
T1462 4034-4049 JJ denotes NF-κB-dependent
T1463 4050-4066 JJ denotes pro-inflammatory
T1464 4067-4071 NN denotes gene
T1465 4072-4085 NN denotes transcription
T1466 4085-4086 , denotes ,
T1467 4087-4099 RB denotes particularly
T1468 4100-4102 IN denotes of
T1469 4103-4106 DT denotes the
T1470 4107-4112 NNP denotes TNF-α
T1471 4113-4117 NN denotes gene
T1472 4117-4119 CD denotes .9
T1473 4120-4131 NNP denotes Controversy
T1474 4132-4138 VBZ denotes exists
T1475 4139-4148 VBG denotes regarding
T1476 4149-4152 DT denotes the
T1477 4153-4158 JJ denotes exact
T1478 4159-4168 NN denotes mechanism
T1479 4168-4169 -LRB- denotes (
T1480 4169-4170 PRP denotes s
T1481 4170-4171 -RRB- denotes )
T1482 4172-4174 IN denotes by
T1483 4175-4180 WDT denotes which
T1484 4181-4184 NNP denotes PDE
T1485 4185-4195 NN denotes inhibition
T1486 4196-4210 VBZ denotes down-regulates
T1487 4211-4216 JJ denotes TNF-α
T1488 4217-4227 NN denotes production
T1489 4227-4228 . denotes .
T1490 4229-4242 NNS denotes Possibilities
T1491 4243-4250 VBP denotes include
T1492 4250-4251 , denotes ,
T1493 4252-4255 CC denotes but
T1494 4256-4259 VBP denotes are
T1495 4260-4263 RB denotes not
T1496 4264-4271 VBN denotes limited
T1497 4272-4274 TO denotes to
T1498 4274-4275 , denotes ,
T1499 4276-4286 NN denotes inhibition
T1500 4287-4289 IN denotes of
T1501 4290-4295 NNP denotes NF-κB
T1502 4296-4299 NNP denotes DNA
T1503 4300-4307 JJ denotes binding
T1504 4308-4316 NN denotes activity
T1505 4316-4317 , denotes ,
T1506 4318-4332 NN denotes downregulation
T1507 4333-4335 IN denotes of
T1508 4336-4341 JJ denotes NF-κB
T1509 4342-4357 JJ denotes transcriptional
T1510 4358-4366 NN denotes activity
T1511 4366-4367 , denotes ,
T1512 4368-4377 VBD denotes increased
T1513 4378-4382 NNP denotes CREB
T1514 4383-4393 NN denotes activation
T1515 4393-4394 , denotes ,
T1516 4395-4398 CC denotes and
T1517 4399-4410 NN denotes competition
T1518 4411-4418 IN denotes between
T1519 4419-4424 NNP denotes NF-κB
T1520 4425-4428 CC denotes and
T1521 4429-4433 NNP denotes CREB
T1522 4434-4437 IN denotes for
T1523 4438-4444 JJ denotes common
T1524 4445-4458 NNS denotes co-activators
T1525 4459-4463 JJ denotes such
T1526 4464-4466 IN denotes as
T1527 4467-4471 NNP denotes CREB
T1528 4472-4479 JJ denotes binding
T1529 4480-4487 NN denotes protein
T1530 4488-4489 -LRB- denotes (
T1531 4489-4492 NNP denotes CBP
T1532 4492-4493 -RRB- denotes )
T1533 4493-4494 . denotes .
T1534 4495-4497 PRP denotes It
T1535 4498-4500 VBZ denotes is
T1536 4501-4505 RB denotes also
T1537 4506-4509 RB denotes not
T1538 4510-4515 VBN denotes known
T1539 4516-4523 IN denotes whether
T1540 4524-4529 DT denotes these
T1541 4530-4539 NNS denotes processes
T1542 4540-4544 VBP denotes rely
T1543 4545-4551 RB denotes solely
T1544 4552-4556 IN denotes upon
T1545 4557-4560 DT denotes the
T1546 4561-4571 NN denotes activation
T1547 4572-4574 IN denotes of
T1548 4575-4578 NNP denotes PKA
T1549 4578-4579 . denotes .
T1550 4580-4589 RB denotes Therefore
T1551 4589-4590 , denotes ,
T1552 4591-4594 DT denotes the
T1553 4595-4604 NN denotes objective
T1554 4605-4607 IN denotes of
T1555 4608-4611 DT denotes the
T1556 4612-4619 JJ denotes present
T1557 4620-4625 NN denotes study
T1558 4626-4628 VBZ denotes is
T1559 4629-4631 TO denotes to
T1560 4632-4641 VB denotes determine
T1561 4642-4645 DT denotes the
T1562 4646-4653 NNS denotes effects
T1563 4654-4656 IN denotes of
T1564 4657-4668 JJ denotes nonspecific
T1565 4669-4672 NNP denotes PDE
T1566 4673-4683 NN denotes inhibition
T1567 4684-4688 IN denotes with
T1568 4689-4690 LS denotes 1
T1569 4690-4691 : denotes -
T1570 4691-4692 NNP denotes [
T1571 4692-4702 JJ denotes 5-oxohexyl
T1572 4702-4703 NNP denotes ]
T1573 4703-4707 CD denotes -3,7
T1574 4707-4708 : denotes -
T1575 4708-4724 NN denotes dimethylxanthine
T1576 4725-4726 -LRB- denotes (
T1577 4726-4740 NNP denotes Pentoxifylline
T1578 4740-4741 : denotes ;
T1579 4742-4745 NNP denotes PTX
T1580 4745-4746 -RRB- denotes )
T1581 4747-4749 IN denotes on
T1582 4750-4755 NN denotes NF-κB
T1583 4756-4759 CC denotes and
T1584 4760-4764 NNP denotes CREB
T1585 4765-4775 NN denotes activation
T1586 4776-4778 IN denotes in
T1587 4779-4784 NN denotes vitro
T1588 4785-4787 IN denotes in
T1589 4788-4793 JJ denotes human
T1590 4794-4805 NN denotes mononuclear
T1591 4806-4811 NNS denotes cells
T1592 4811-4812 . denotes .
T1593 4813-4817 IN denotes With
T1594 4818-4821 DT denotes the
T1595 4822-4825 NN denotes use
T1596 4826-4828 IN denotes of
T1597 4829-4837 JJ denotes specific
T1598 4838-4848 NNS denotes inhibitors
T1599 4848-4849 , denotes ,
T1600 4850-4852 PRP denotes we
T1601 4853-4857 RB denotes also
T1602 4858-4870 VBD denotes investigated
T1603 4871-4874 DT denotes the
T1604 4875-4879 NN denotes role
T1605 4880-4882 IN denotes of
T1606 4883-4886 NNP denotes PKA
T1607 4887-4889 IN denotes in
T1608 4890-4901 JJ denotes LPS-induced
T1609 4902-4907 JJ denotes TNF-α
T1610 4908-4918 NN denotes production
T1611 4918-4919 . denotes .
T2464 172-176 DT denotes this
T2465 177-182 NN denotes study
T2466 183-186 VBD denotes was
T2467 4958-4966 VBN denotes approved
T2468 4967-4969 IN denotes by
T2469 4970-4973 DT denotes the
T2470 4974-4979 NNP denotes Human
T2471 4980-4988 NNP denotes Research
T2472 4989-5000 NNPS denotes Protections
T2473 5001-5008 NNP denotes Program
T2474 5009-5012 CC denotes and
T2475 5013-5016 DT denotes the
T2476 5017-5030 JJ denotes Institutional
T2477 5031-5037 NNP denotes Review
T2478 5038-5043 NNP denotes Board
T2479 5043-5044 . denotes .
T2480 5045-5052 VBN denotes Written
T2481 5053-5060 NN denotes consent
T2482 5061-5063 TO denotes to
T2483 5064-5075 VB denotes participate
T2484 5076-5078 IN denotes in
T2485 5079-5082 DT denotes the
T2486 5083-5088 NN denotes study
T2487 5089-5092 VBD denotes was
T2488 5093-5101 VBN denotes obtained
T2489 5102-5106 IN denotes from
T2490 5107-5110 DT denotes all
T2491 5111-5121 NNS denotes volunteers
T2492 5122-5127 RB denotes prior
T2493 5128-5130 TO denotes to
T2494 5131-5136 NN denotes blood
T2495 5137-5158 VBN denotes donation. Heparinized
T2496 5159-5170 NNS denotes Vacutainers
T2497 5171-5175 VBD denotes were
T2498 5176-5185 VBN denotes purchased
T2499 5186-5190 IN denotes from
T2500 5191-5197 NNP denotes Becton
T2501 5198-5207 NNP denotes Dickinson
T2502 5208-5209 -LRB- denotes (
T2503 5209-5212 NNP denotes San
T2504 5213-5217 NNP denotes Jose
T2505 5217-5218 , denotes ,
T2506 5219-5221 NNP denotes CA
T2507 5221-5222 -RRB- denotes )
T2508 5222-5223 . denotes .
T2509 5224-5230 CD denotes 1.5-mL
T2510 5231-5240 NNP denotes Eppendorf
T2511 5241-5251 NN denotes centrifuge
T2512 5252-5257 NNS denotes tubes
T2513 5258-5262 VBD denotes were
T2514 5263-5272 VBN denotes purchased
T2515 5273-5277 IN denotes from
T2516 5278-5284 NNP denotes Fisher
T2517 5285-5295 NNP denotes Scientific
T2518 5296-5297 -LRB- denotes (
T2519 5297-5306 NNP denotes Pittsburg
T2520 5306-5307 , denotes ,
T2521 5308-5310 NNP denotes PA
T2522 5310-5311 -RRB- denotes )
T2523 5311-5312 . denotes .
T2524 5313-5317 NNP denotes Hank
T2525 5320-5328 JJ denotes balanced
T2526 5329-5333 NN denotes salt
T2527 5334-5342 NN denotes solution
T2528 5343-5344 -LRB- denotes (
T2529 5344-5348 NNP denotes HBSS
T2530 5348-5349 -RRB- denotes )
T2531 5350-5353 CC denotes and
T2532 5354-5358 NNP denotes RPMI
T2533 5359-5363 CD denotes 1640
T2534 5364-5368 VBD denotes were
T2535 5369-5377 VBN denotes obtained
T2536 5378-5382 IN denotes from
T2537 5383-5389 NNP denotes Irvine
T2538 5390-5400 NNP denotes Scientific
T2539 5401-5402 -LRB- denotes (
T2540 5402-5407 NNP denotes Santa
T2541 5408-5411 NNP denotes Ana
T2542 5411-5412 , denotes ,
T2543 5413-5415 NNP denotes CA
T2544 5415-5416 -RRB- denotes )
T2545 5416-5417 . denotes .
T2546 5418-5421 NNP denotes LPS
T2547 5422-5438 NNP denotes from Escherichia
T2548 5439-5452 NN denotes coli serotype
T2549 5453-5457 CD denotes 0111
T2550 5457-5458 : denotes :
T2551 5458-5460 NN denotes B4
T2552 5461-5464 CC denotes and
T2553 5465-5468 NNP denotes PTX
T2554 5469-5473 VBD denotes were
T2555 5474-5483 VBN denotes purchased
T2556 5484-5488 IN denotes from
T2557 5489-5494 NNP denotes Sigma
T2558 5495-5496 -LRB- denotes (
T2559 5496-5499 NNP denotes St.
T2560 5500-5505 NNP denotes Louis
T2561 5505-5506 , denotes ,
T2562 5507-5509 NNP denotes MO
T2563 5509-5510 -RRB- denotes )
T2564 5510-5511 . denotes .
T2565 5512-5515 DT denotes The
T2566 5516-5519 NNP denotes PKA
T2567 5520-5529 NN denotes inhibitor
T2568 5529-5530 , denotes ,
T2569 5531-5532 NNP denotes N
T2570 5532-5533 : denotes -
T2571 5533-5534 NN denotes [
T2572 5534-5535 LS denotes 2
T2573 5535-5536 : denotes -
T2574 5536-5537 -LRB- denotes (
T2575 5537-5538 -LRB- denotes (
T2576 5538-5553 NN denotes p-bromocinnamyl
T2577 5553-5554 -RRB- denotes )
T2578 5554-5559 JJ denotes amino
T2579 5559-5560 -RRB- denotes )
T2580 5560-5565 FW denotes ethyl
T2581 5565-5566 NNP denotes ]
T2582 5566-5568 SYM denotes -5
T2583 5568-5569 : denotes -
T2584 5569-5597 NN denotes isoguinolinesulfonamide-2HCl
T2585 5597-5598 NNP denotes ]
T2586 5599-5600 -LRB- denotes (
T2587 5600-5603 NNP denotes H89
T2588 5603-5604 -RRB- denotes )
T2589 5604-5605 , denotes ,
T2590 5606-5609 VBD denotes was
T2591 5610-5619 VBN denotes purchased
T2592 5620-5624 IN denotes from
T2593 5625-5635 NNP denotes Calbiochem
T2594 5636-5637 -LRB- denotes (
T2595 5637-5639 NNP denotes La
T2596 5640-5645 NNP denotes Jolla
T2597 5645-5646 , denotes ,
T2598 5647-5649 NNP denotes CA
T2599 5649-5650 -RRB- denotes )
T2600 5650-5651 . denotes .
T2601 5652-5662 NNS denotes Antibodies
T2602 5663-5666 IN denotes for
T2603 5667-5681 VBN denotes phosphorylated
T2604 5682-5686 NNP denotes CREB
T2605 5687-5688 -LRB- denotes (
T2606 5688-5694 NN denotes serine
T2607 5695-5698 CD denotes 133
T2608 5698-5699 -RRB- denotes )
T2609 5699-5700 , denotes ,
T2610 5701-5715 VBN denotes phosphorylated
T2611 5716-5721 NNP denotes I-κBα
T2612 5722-5723 -LRB- denotes (
T2613 5723-5730 NNS denotes serines
T2614 5731-5733 CD denotes 32
T2615 5734-5737 CC denotes and
T2616 5738-5740 CD denotes 36
T2617 5740-5741 -RRB- denotes )
T2618 5741-5742 , denotes ,
T2619 5743-5757 VBN denotes phosphorylated
T2620 5758-5763 NNP denotes NF-κB
T2621 5764-5767 CD denotes p65
T2622 5768-5775 NN denotes subunit
T2623 5776-5777 -LRB- denotes (
T2624 5777-5783 NN denotes serine
T2625 5784-5787 CD denotes 276
T2626 5787-5788 -RRB- denotes )
T2627 5788-5789 , denotes ,
T2628 5790-5793 CC denotes and
T2629 5794-5803 JJ denotes secondary
T2630 5804-5814 NNS denotes antibodies
T2631 5815-5819 VBD denotes were
T2632 5820-5829 VBN denotes purchased
T2633 5830-5834 IN denotes from
T2634 5835-5839 NNP denotes Cell
T2635 5840-5849 NNP denotes Signaling
T2636 5850-5851 -LRB- denotes (
T2637 5851-5858 NNP denotes Beverly
T2638 5858-5859 , denotes ,
T2639 5860-5862 NNP denotes MA
T2640 5862-5863 -RRB- denotes )
T2641 5863-5864 . denotes .
T2642 5865-5872 JJ denotes Dextran
T2643 5873-5877 CD denotes T500
T2644 5878-5881 CC denotes and
T2645 5882-5889 NNP denotes Percoll
T2646 5890-5894 VBD denotes were
T2647 5895-5903 VBN denotes received
T2648 5904-5908 IN denotes from
T2649 5909-5911 NNP denotes GE
T2650 5912-5931 NNP denotes Healthcare/Amersham
T2651 5932-5943 NNP denotes Biosciences
T2652 5944-5945 -LRB- denotes (
T2653 5945-5955 NNP denotes Piscataway
T2654 5955-5956 , denotes ,
T2655 5957-5959 NNP denotes NJ
T2656 5959-5960 -RRB- denotes )
T2657 5960-5961 . denotes .
T2658 5962-5965 DT denotes The
T2659 5966-5979 JJ denotes enzyme-linked
T2660 5980-5993 JJ denotes immunosorbent
T2661 5994-5999 NN denotes assay
T2662 6000-6001 -LRB- denotes (
T2663 6001-6006 NNP denotes ELISA
T2664 6006-6007 -RRB- denotes )
T2665 6008-6011 IN denotes for
T2666 6012-6017 NNP denotes TNF-α
T2667 6018-6021 VBD denotes was
T2668 6022-6030 VBN denotes obtained
T2669 6031-6035 IN denotes from
T2670 6036-6046 NNP denotes Quantikine
T2671 6047-6050 NNP denotes R&D
T2672 6051-6058 NNP denotes Systems
T2673 6059-6060 -LRB- denotes (
T2674 6060-6071 NNP denotes Minneapolis
T2675 6071-6072 , denotes ,
T2676 6073-6075 NNP denotes MN
T2677 6075-6076 -RRB- denotes )
T2678 6076-6077 . denotes .
T2679 6078-6090 CD denotes Tris-glycine
T2680 6091-6094 CC denotes and
T2681 6095-6098 NNP denotes DNA
T2682 6099-6110 NN denotes retardation
T2683 6111-6115 NNS denotes gels
T2684 6115-6116 , denotes ,
T2685 6117-6131 JJ denotes nitrocellulose
T2686 6132-6141 NNS denotes membranes
T2687 6141-6142 , denotes ,
T2688 6143-6146 CC denotes and
T2689 6147-6154 JJ denotes western
T2690 6155-6159 NN denotes blot
T2691 6160-6167 VBG denotes running
T2692 6168-6175 NNS denotes buffers
T2693 6176-6180 VBD denotes were
T2694 6181-6189 VBN denotes obtained
T2695 6190-6194 IN denotes from
T2696 6195-6205 NNP denotes Invitrogen
T2697 6206-6207 -LRB- denotes (
T2698 6207-6215 NNP denotes Carlsbad
T2699 6215-6216 , denotes ,
T2700 6217-6219 NNP denotes CA
T2701 6219-6220 -RRB- denotes )
T2702 6220-6221 . denotes .
T2703 6222-6228 NNP denotes NE-PER
T2704 6228-6229 NNP denotes ®
T2705 6230-6237 JJ denotes nuclear
T2706 6238-6241 CC denotes and
T2707 6242-6253 JJ denotes cytoplasmic
T2708 6254-6264 NN denotes extraction
T2709 6265-6273 NNS denotes reagents
T2710 6273-6274 , denotes ,
T2711 6275-6278 DT denotes the
T2712 6279-6282 NNP denotes BCA
T2713 6283-6290 NN denotes protein
T2714 6291-6298 NN denotes reagent
T2715 6299-6302 NN denotes kit
T2716 6302-6303 , denotes ,
T2717 6304-6307 DT denotes the
T2718 6308-6319 NNP denotes Supersignal
T2719 6320-6324 NNP denotes West
T2720 6325-6329 NNP denotes Pico
T2721 6330-6346 NNP denotes Chemiluminescent
T2722 6347-6350 NNP denotes Kit
T2723 6350-6351 , denotes ,
T2724 6352-6355 CC denotes and
T2725 6356-6359 DT denotes the
T2726 6360-6370 NNP denotes LightShift
T2727 6371-6387 NNP denotes Chemiluminescent
T2728 6388-6392 NNP denotes EMSA
T2729 6393-6396 NN denotes Kit
T2730 6397-6401 VBD denotes were
T2731 6402-6411 VBN denotes purchased
T2732 6412-6416 IN denotes from
T2733 6417-6423 NNP denotes Pierce
T2734 6424-6425 -LRB- denotes (
T2735 6425-6433 NNP denotes Rockland
T2736 6433-6434 , denotes ,
T2737 6435-6437 NNP denotes IL
T2738 6437-6438 -RRB- denotes )
T2739 6438-6439 . denotes .
T2740 6440-6444 NNP denotes CREB
T2741 6445-6448 CC denotes and
T2742 6449-6454 NNP denotes NF-κB
T2743 6455-6458 NNP denotes DNA
T2744 6459-6474 NN denotes oligonucleotide
T2745 6475-6481 NNS denotes probes
T2746 6482-6486 VBD denotes were
T2747 6487-6498 VBN denotes synthesized
T2748 6499-6501 IN denotes by
T2749 6502-6505 NNP denotes IDT
T2750 6506-6507 -LRB- denotes (
T2751 6507-6517 NNP denotes Coralville
T2752 6517-6518 , denotes ,
T2753 6519-6521 NNP denotes IA
T2754 6521-6522 -RRB- denotes )
T2755 6522-6523 . denotes .
T2756 6524-6529 NNP denotes Nylon
T2757 6530-6539 NNS denotes membranes
T2758 6540-6544 VBD denotes were
T2759 6545-6554 VBN denotes purchased
T2760 6555-6559 IN denotes from
T2761 6560-6565 NNP denotes Roche
T2762 6566-6573 NNP denotes Applied
T2763 6574-6582 NNP denotes Sciences
T2764 6583-6584 -LRB- denotes (
T2765 6584-6596 NNP denotes Indianapolis
T2766 6596-6597 , denotes ,
T2767 6598-6600 IN denotes IN
T2768 6600-6601 -RRB- denotes )
T2769 6601-6602 . denotes .
T2770 6603-6610 JJ denotes Western
T2771 6611-6615 NN denotes blot
T2772 6616-6620 NN denotes band
T2773 6621-6635 NN denotes quantification
T2774 6636-6639 VBD denotes was
T2775 6640-6649 VBN denotes performed
T2776 6650-6654 IN denotes with
T2777 6655-6658 DT denotes the
T2778 6659-6669 NNP denotes UN-SCAN-IT
T2779 6670-6673 NNP denotes Gel
T2780 6674-6684 NNP denotes Digitizing
T2781 6685-6693 NN denotes software
T2782 6694-6695 -LRB- denotes (
T2783 6695-6699 NNP denotes Silk
T2784 6700-6710 NNP denotes Scientific
T2785 6710-6711 , denotes ,
T2786 6712-6716 NNP denotes Orem
T2787 6716-6717 , denotes ,
T2788 6718-6720 NNP denotes UT
T2789 6720-6721 -RRB- denotes )
T2790 6721-6722 . denotes .
T3267 6724-6729 NNP denotes Human
T3268 6730-6740 NNP denotes Peripheral
T3269 6741-6746 NNP denotes Blood
T3270 6747-6758 NNP denotes Mononuclear
T3271 6759-6763 NNP denotes Cell
T3272 6764-6773 NNP denotes Isolation
T3273 6774-6777 CC denotes and
T3274 6778-6789 NNP denotes Stimulation
T3275 6790-6795 NNP denotes Human
T3276 6796-6807 NN denotes mononuclear
T3277 6808-6813 NNS denotes cells
T3278 6814-6818 VBD denotes were
T3279 6819-6827 VBN denotes isolated
T3280 6828-6832 IN denotes from
T3281 6833-6836 DT denotes the
T3282 6837-6847 JJ denotes peripheral
T3283 6848-6853 NN denotes blood
T3284 6854-6856 IN denotes of
T3285 6857-6861 CD denotes four
T3286 6862-6869 JJ denotes healthy
T3287 6870-6875 JJ denotes human
T3288 6876-6886 NNS denotes volunteers
T3289 6886-6887 . denotes .
T3290 6888-6891 DT denotes The
T3291 6892-6898 NN denotes sample
T3292 6899-6903 NN denotes size
T3293 6904-6907 VBD denotes was
T3294 6908-6914 VBN denotes chosen
T3295 6915-6919 IN denotes from
T3296 6920-6923 PRP$ denotes our
T3297 6924-6934 RB denotes previously
T3298 6935-6944 VBN denotes published
T3299 6945-6949 NN denotes work
T3300 6949-6950 , denotes ,
T3301 6951-6956 WDT denotes which
T3302 6957-6969 VBD denotes demonstrated
T3303 6970-6978 JJ denotes reliable
T3304 6979-6986 NNS denotes results
T3305 6986-6989 CD denotes .10
T3306 6990-6994 NNP denotes Cell
T3307 6995-7004 NN denotes isolation
T3308 7005-7008 CC denotes and
T3309 7009-7012 DT denotes all
T3310 7013-7023 JJ denotes subsequent
T3311 7024-7035 NNS denotes experiments
T3312 7036-7040 VBD denotes were
T3313 7041-7050 VBN denotes conducted
T3314 7051-7056 IN denotes under
T3315 7057-7064 JJ denotes sterile
T3316 7065-7068 CC denotes and
T3317 7069-7081 JJ denotes pyrogen-free
T3318 7082-7092 NNS denotes conditions
T3319 7092-7093 . denotes .
T3320 7094-7099 NNP denotes Blood
T3321 7100-7109 VBN denotes collected
T3322 7110-7112 IN denotes in
T3323 7113-7120 NN denotes heparin
T3324 7121-7126 NNS denotes tubes
T3325 7127-7130 VBD denotes was
T3326 7131-7140 VBN denotes incubated
T3327 7141-7145 IN denotes with
T3328 7146-7153 NNP denotes Dextran
T3329 7154-7158 CD denotes T500
T3330 7159-7162 CC denotes and
T3331 7163-7166 DT denotes the
T3332 7167-7170 JJ denotes red
T3333 7171-7176 NNS denotes cells
T3334 7177-7181 VBD denotes were
T3335 7182-7192 VBN denotes sedimented
T3336 7193-7196 IN denotes for
T3337 7197-7199 CD denotes 40
T3338 7200-7207 NNS denotes minutes
T3339 7208-7210 IN denotes at
T3340 7211-7215 NN denotes room
T3341 7216-7227 NN denotes temperature
T3342 7227-7228 . denotes .
T3343 7229-7232 DT denotes The
T3344 7233-7242 JJ denotes resultant
T3345 7243-7248 NN denotes serum
T3346 7249-7252 VBD denotes was
T3347 7253-7257 RB denotes then
T3348 7258-7264 VBN denotes washed
T3349 7265-7269 IN denotes with
T3350 7270-7274 NNP denotes HBSS
T3351 7275-7278 CC denotes and
T3352 7279-7288 VBN denotes separated
T3353 7289-7291 IN denotes by
T3354 7292-7299 NNP denotes Percoll
T3355 7300-7308 NN denotes gradient
T3356 7309-7323 NN denotes centrifugation
T3357 7324-7333 VBG denotes according
T3358 7334-7336 TO denotes to
T3359 7337-7340 DT denotes the
T3360 7341-7353 NN denotes manufacturer
T3361 7356-7368 NNS denotes instructions
T3362 7368-7369 . denotes .
T3363 7370-7374 NNP denotes Cell
T3364 7375-7384 NN denotes viability
T3365 7385-7388 VBD denotes was
T3366 7389-7397 VBN denotes assessed
T3367 7398-7400 IN denotes by
T3368 7401-7407 NN denotes trypan
T3369 7408-7412 JJ denotes blue
T3370 7413-7416 NN denotes dye
T3371 7417-7426 NN denotes exclusion
T3372 7426-7427 , denotes ,
T3373 7428-7432 IN denotes with
T3374 7433-7439 NN denotes purity
T3375 7440-7442 IN denotes of
T3376 7443-7450 JJR denotes greater
T3377 7451-7455 IN denotes than
T3378 7456-7458 CD denotes 95
T3379 7458-7459 NN denotes %
T3380 7459-7460 . denotes .
T3381 7461-7469 VBN denotes Isolated
T3382 7470-7475 NNS denotes cells
T3383 7476-7480 VBD denotes were
T3384 7481-7492 VBN denotes resuspended
T3385 7493-7495 IN denotes in
T3386 7496-7500 NNP denotes RPMI
T3387 7501-7505 CD denotes 1640
T3388 7506-7518 VBN denotes supplemented
T3389 7519-7523 IN denotes with
T3390 7524-7526 CD denotes 10
T3391 7526-7527 NN denotes %
T3392 7528-7531 NNP denotes FBS
T3393 7532-7535 CC denotes and
T3394 7536-7537 CD denotes 5
T3395 7538-7540 NNP denotes mM
T3396 7541-7546 NNP denotes HEPES
T3397 7547-7549 IN denotes at
T3398 7550-7551 DT denotes a
T3399 7552-7565 NN denotes concentration
T3400 7566-7568 IN denotes of
T3401 7569-7570 CD denotes 1
T3402 7571-7572 LS denotes x
T3403 7573-7584 NNP denotes 107cells/mL
T3404 7584-7585 . denotes .
T3405 7586-7590 DT denotes Each
T3406 7591-7601 JJ denotes subsequent
T3407 7602-7612 NN denotes experiment
T3408 7613-7619 VBN denotes listed
T3409 7620-7625 RB denotes below
T3410 7626-7629 VBD denotes was
T3411 7630-7639 VBN denotes conducted
T3412 7640-7642 IN denotes on
T3413 7643-7650 NNS denotes samples
T3414 7651-7653 IN denotes of
T3415 7654-7655 CD denotes 5
T3416 7656-7657 LS denotes x
T3417 7658-7661 CD denotes 106
T3418 7662-7667 NNS denotes cells
T3419 7668-7671 IN denotes per
T3420 7672-7681 NN denotes treatment
T3421 7682-7687 NN denotes group
T3422 7687-7688 . denotes .
T3423 7689-7697 VBN denotes Isolated
T3424 7698-7703 NNS denotes cells
T3425 7704-7708 VBD denotes were
T3426 7709-7719 VBN denotes stimulated
T3427 7720-7724 IN denotes with
T3428 7725-7731 DT denotes either
T3429 7732-7736 NNP denotes HBSS
T3430 7737-7739 IN denotes as
T3431 7740-7741 DT denotes a
T3432 7742-7750 JJ denotes negative
T3433 7751-7758 NN denotes control
T3434 7758-7759 , denotes ,
T3435 7760-7763 NNP denotes LPS
T3436 7764-7765 -LRB- denotes (
T3437 7765-7766 CD denotes 1
T3438 7767-7769 NN denotes μg
T3439 7769-7770 NN denotes /
T3440 7770-7772 NNP denotes mL
T3441 7772-7773 -RRB- denotes )
T3442 7773-7774 , denotes ,
T3443 7775-7778 NNP denotes PTX
T3444 7779-7780 -LRB- denotes (
T3445 7780-7782 CD denotes 20
T3446 7783-7785 NNP denotes mM
T3447 7785-7786 -RRB- denotes )
T3448 7786-7787 , denotes ,
T3449 7788-7790 CC denotes or
T3450 7791-7802 JJ denotes concomitant
T3451 7803-7806 NNP denotes LPS
T3452 7807-7808 NN denotes /
T3453 7809-7812 NNP denotes PTX
T3454 7812-7813 , denotes ,
T3455 7814-7816 IN denotes at
T3456 7817-7820 DT denotes the
T3457 7821-7836 JJ denotes above-mentioned
T3458 7837-7851 NNS denotes concentrations
T3459 7851-7852 , denotes ,
T3460 7853-7856 IN denotes for
T3461 7857-7859 CD denotes 30
T3462 7860-7867 NNS denotes minutes
T3463 7868-7870 IN denotes at
T3464 7871-7875 NNP denotes 37ºC
T3465 7875-7876 . denotes .
T3466 7877-7882 NNS denotes Cells
T3467 7883-7887 VBD denotes were
T3468 7888-7894 VBN denotes placed
T3469 7895-7897 IN denotes on
T3470 7898-7901 NN denotes ice
T3471 7902-7905 IN denotes for
T3472 7906-7908 CD denotes 10
T3473 7909-7916 NNS denotes minutes
T3474 7917-7919 TO denotes to
T3475 7920-7924 VB denotes stop
T3476 7925-7928 DT denotes the
T3477 7929-7937 NN denotes reaction
T3478 7938-7941 CC denotes and
T3479 7942-7945 DT denotes the
T3480 7946-7953 NNS denotes samples
T3481 7954-7958 VBD denotes were
T3482 7959-7963 RB denotes then
T3483 7964-7970 VBN denotes stored
T3484 7971-7973 IN denotes at
T3485 7974-7975 CD denotes −
T3486 7975-7977 CD denotes 70
T3487 7977-7978 NN denotes °
T3488 7978-7979 NNP denotes C
T3489 7980-7983 IN denotes for
T3490 7984-7991 JJ denotes further
T3491 7992-8000 NN denotes analysis
T3492 8000-8001 . denotes .
T3493 8002-8005 DT denotes The
T3494 8006-8017 NN denotes stimulation
T3495 8018-8023 NNS denotes times
T3496 8024-8027 CC denotes and
T3497 8028-8042 NNS denotes concentrations
T3498 8043-8045 IN denotes of
T3499 8046-8049 NNP denotes LPS
T3500 8050-8053 CC denotes and
T3501 8054-8057 NNP denotes PTX
T3502 8058-8066 VBD denotes utilized
T3503 8067-8069 IN denotes in
T3504 8070-8074 DT denotes this
T3505 8075-8080 NN denotes study
T3506 8081-8085 VBD denotes were
T3507 8086-8096 VBN denotes determined
T3508 8097-8099 IN denotes in
T3509 8100-8108 JJ denotes previous
T3510 8109-8114 NN denotes pilot
T3511 8115-8122 NNS denotes studies
T3512 8123-8127 VBN denotes done
T3513 8128-8130 IN denotes in
T3514 8131-8134 PRP$ denotes our
T3515 8135-8145 NN denotes laboratory
T3516 8145-8146 , denotes ,
T3517 8147-8152 WDT denotes which
T3518 8153-8161 VBD denotes examined
T3519 8162-8165 DT denotes the
T3520 8166-8173 NNS denotes effects
T3521 8174-8176 IN denotes of
T3522 8177-8187 VBG denotes increasing
T3523 8188-8202 NNS denotes concentrations
T3524 8203-8205 IN denotes of
T3525 8206-8209 NNP denotes PTX
T3526 8210-8212 IN denotes on
T3527 8213-8224 JJ denotes LPS-induced
T3528 8225-8230 JJ denotes TNF-α
T3529 8231-8241 NN denotes production
T3530 8241-8242 . denotes .
T3531 8243-8246 NNP denotes LPS
T3532 8247-8249 IN denotes at
T3533 8250-8251 DT denotes a
T3534 8252-8265 NN denotes concentration
T3535 8266-8268 IN denotes of
T3536 8269-8270 CD denotes 1
T3537 8271-8273 NN denotes μg
T3538 8273-8274 NN denotes /
T3539 8274-8276 NNP denotes mL
T3540 8277-8280 CC denotes and
T3541 8281-8284 NNP denotes PTX
T3542 8285-8287 IN denotes at
T3543 8288-8289 DT denotes a
T3544 8290-8303 NN denotes concentration
T3545 8304-8306 IN denotes of
T3546 8307-8309 CD denotes 20
T3547 8310-8312 NN denotes mM
T3548 8313-8317 VBD denotes were
T3549 8318-8321 DT denotes the
T3550 8322-8329 JJ denotes minimum
T3551 8330-8344 NNS denotes concentrations
T3552 8345-8354 JJ denotes necessary
T3553 8355-8357 TO denotes to
T3554 8358-8365 VB denotes produce
T3555 8366-8367 DT denotes a
T3556 8368-8380 JJ denotes reproducible
T3557 8381-8384 CC denotes and
T3558 8385-8393 JJ denotes reliable
T3559 8394-8407 NN denotes up-regulation
T3560 8408-8411 CC denotes and
T3561 8412-8427 NN denotes down-regulation
T3562 8428-8430 IN denotes in
T3563 8431-8436 JJ denotes TNF-α
T3564 8437-8447 NN denotes production
T3565 8447-8448 , denotes ,
T3566 8449-8461 RB denotes respectively
T3567 8461-8462 , denotes ,
T3568 8463-8465 IN denotes in
T3569 8466-8478 JJ denotes quantitative
T3570 8479-8485 NNS denotes assays
T3571 8486-8487 -LRB- denotes (
T3572 8487-8491 NNS denotes data
T3573 8492-8495 RB denotes not
T3574 8496-8501 VBN denotes shown
T3575 8501-8502 -RRB- denotes )
T3576 8502-8503 . denotes .
T3971 8505-8511 NN denotes Effect
T3972 8512-8514 IN denotes of
T3973 8515-8518 NNP denotes PTX
T3974 8519-8521 IN denotes on
T3975 8522-8533 JJ denotes LPS-induced
T3976 8534-8539 JJ denotes TNF-α
T3977 8540-8550 NN denotes production
T3978 8551-8553 IN denotes by
T3979 8554-8565 NN denotes mononuclear
T3980 8566-8571 NNS denotes cells
T3981 8572-8575 DT denotes The
T3982 8576-8589 NN denotes concentration
T3983 8590-8592 IN denotes of
T3984 8593-8598 NNP denotes TNF-α
T3985 8599-8602 VBD denotes was
T3986 8603-8617 RB denotes quantitatively
T3987 8618-8628 VBN denotes determined
T3988 8629-8631 IN denotes by
T3989 8632-8637 NNP denotes ELISA
T3990 8638-8640 IN denotes in
T3991 8641-8644 DT denotes the
T3992 8645-8650 NNS denotes media
T3993 8651-8653 IN denotes of
T3994 8654-8659 NNS denotes cells
T3995 8660-8667 VBN denotes exposed
T3996 8668-8670 TO denotes to
T3997 8671-8674 DT denotes the
T3998 8675-8685 NNS denotes treatments
T3999 8686-8695 VBN denotes described
T4000 8696-8701 IN denotes above
T4001 8702-8707 IN denotes after
T4002 8708-8719 NN denotes stimulation
T4003 8720-8723 IN denotes for
T4004 8724-8726 RB denotes up
T4005 8727-8729 TO denotes to
T4006 8730-8731 DT denotes a
T4007 8732-8739 NN denotes maximum
T4008 8740-8742 IN denotes of
T4009 8743-8745 CD denotes 18
T4010 8746-8751 NNS denotes hours
T4011 8751-8752 , denotes ,
T4012 8753-8755 IN denotes at
T4013 8756-8762 JJ denotes 3-hour
T4014 8763-8772 NNS denotes intervals
T4015 8772-8773 . denotes .
T4016 8774-8777 DT denotes The
T4017 8778-8785 NNS denotes results
T4018 8786-8789 VBP denotes are
T4019 8790-8799 VBN denotes expressed
T4020 8800-8802 IN denotes in
T4021 8803-8808 NNP denotes pg/mL
T4022 8808-8809 . denotes .
T4152 8811-8819 NNP denotes SDS-PAGE
T4153 8820-8823 CC denotes and
T4154 8824-8838 NNP denotes Immunoblotting
T4155 8839-8840 -LRB- denotes (
T4156 8840-8845 NNP denotes I-κBα
T4157 8845-8846 , denotes ,
T4158 8847-8852 NNP denotes NF-κB
T4159 8853-8856 CD denotes p65
T4160 8856-8857 , denotes ,
T4161 8858-8861 CC denotes and
T4162 8862-8866 NNP denotes CREB
T4163 8866-8867 -RRB- denotes )
T4164 8868-8877 VBG denotes Following
T4165 8878-8889 NN denotes stimulation
T4166 8889-8890 , denotes ,
T4167 8891-8896 NNS denotes PBMCs
T4168 8897-8901 VBD denotes were
T4169 8902-8908 VBN denotes washed
T4170 8909-8913 IN denotes with
T4171 8914-8922 JJ denotes ice-cold
T4172 8923-8926 NNP denotes PBS
T4173 8927-8930 CC denotes and
T4174 8931-8942 VBD denotes centrifuged
T4175 8943-8945 TO denotes to
T4176 8946-8953 VB denotes collect
T4177 8954-8955 DT denotes a
T4178 8956-8960 NN denotes cell
T4179 8961-8967 NN denotes pellet
T4180 8967-8968 . denotes .
T4181 8969-8972 DT denotes The
T4182 8973-8979 NN denotes pellet
T4183 8980-8983 VBD denotes was
T4184 8984-8995 VBN denotes resuspended
T4185 8996-8998 IN denotes in
T4186 8999-9007 JJ denotes ice-cold
T4187 9008-9011 NNP denotes SDS
T4188 9012-9018 NN denotes sample
T4189 9019-9025 NN denotes buffer
T4190 9026-9038 VBN denotes supplemented
T4191 9039-9043 IN denotes with
T4192 9044-9047 CD denotes 100
T4193 9048-9050 NNP denotes mM
T4194 9051-9065 NN denotes dithiothreitol
T4195 9065-9066 . denotes .
T4196 9067-9078 NNP denotes Cytoplasmic
T4197 9079-9082 CC denotes and
T4198 9083-9090 JJ denotes nuclear
T4199 9091-9099 NNS denotes extracts
T4200 9100-9104 VBD denotes were
T4201 9105-9113 VBN denotes isolated
T4202 9114-9118 IN denotes with
T4203 9119-9125 NNP denotes NE-PER
T4204 9126-9133 JJ denotes nuclear
T4205 9134-9137 CC denotes and
T4206 9138-9149 JJ denotes cytoplasmic
T4207 9150-9160 NN denotes extraction
T4208 9161-9169 VBZ denotes reagents
T4209 9170-9174 IN denotes with
T4210 9175-9177 CD denotes 1x
T4211 9178-9182 NNP denotes Halt
T4212 9183-9191 NNP denotes Protease
T4213 9192-9201 NNP denotes Inhibitor
T4214 9202-9210 NNP denotes Cocktail
T4215 9211-9220 VBG denotes according
T4216 9221-9223 TO denotes to
T4217 9224-9227 DT denotes the
T4218 9228-9240 NN denotes manufacturer
T4219 9243-9255 NNS denotes instructions
T4220 9255-9256 . denotes .
T4221 9257-9264 NN denotes Protein
T4222 9265-9279 NNS denotes concentrations
T4223 9280-9284 VBD denotes were
T4224 9285-9295 VBN denotes determined
T4225 9296-9300 IN denotes with
T4226 9301-9304 DT denotes the
T4227 9305-9308 NNP denotes BCA
T4228 9309-9316 NN denotes protein
T4229 9317-9324 NN denotes reagent
T4230 9325-9328 NN denotes kit
T4231 9329-9332 IN denotes for
T4232 9333-9337 DT denotes each
T4233 9338-9344 NN denotes sample
T4234 9345-9354 VBG denotes according
T4235 9355-9357 TO denotes to
T4236 9358-9359 DT denotes a
T4237 9360-9372 JJ denotes standardized
T4238 9373-9378 NN denotes curve
T4239 9379-9382 IN denotes for
T4240 9383-9390 NN denotes albumin
T4241 9390-9391 . denotes .
T4242 9392-9396 NN denotes Cell
T4243 9397-9404 NNS denotes lysates
T4244 9405-9409 VBD denotes were
T4245 9410-9419 VBN denotes collected
T4246 9420-9422 IN denotes by
T4247 9423-9430 VBG denotes boiling
T4248 9431-9434 DT denotes the
T4249 9435-9442 NNS denotes samples
T4250 9443-9445 IN denotes in
T4251 9446-9447 DT denotes a
T4252 9448-9453 JJ denotes 100ºC
T4253 9454-9459 NN denotes water
T4254 9460-9464 NN denotes bath
T4255 9465-9468 IN denotes for
T4256 9469-9470 CD denotes 5
T4257 9471-9478 NNS denotes minutes
T4258 9478-9479 . denotes .
T4259 9480-9483 CD denotes Ten
T4260 9484-9486 NN denotes μg
T4261 9487-9489 IN denotes of
T4262 9490-9497 NN denotes protein
T4263 9498-9501 IN denotes per
T4264 9502-9508 NN denotes sample
T4265 9509-9512 VBD denotes was
T4266 9513-9522 VBN denotes separated
T4267 9523-9525 IN denotes by
T4268 9526-9544 JJ denotes SDS-polyacrylamide
T4269 9545-9548 NN denotes gel
T4270 9549-9564 NN denotes electrophoresis
T4271 9565-9572 IN denotes through
T4272 9573-9574 CD denotes 8
T4273 9575-9577 CD denotes 16
T4274 9577-9578 NN denotes %
T4275 9579-9591 JJ denotes tris-glycine
T4276 9592-9606 NN denotes polyacrylamide
T4277 9607-9615 NN denotes gradient
T4278 9616-9620 NNS denotes gels
T4279 9621-9624 CC denotes and
T4280 9625-9636 VBN denotes transferred
T4281 9637-9639 TO denotes to
T4282 9640-9654 JJ denotes nitrocellulose
T4283 9655-9664 NNS denotes membranes
T4284 9664-9665 . denotes .
T4285 9666-9669 DT denotes The
T4286 9670-9679 NNS denotes membranes
T4287 9680-9684 VBD denotes were
T4288 9685-9692 VBN denotes blocked
T4289 9693-9697 IN denotes with
T4290 9698-9699 CD denotes 5
T4291 9699-9700 NN denotes %
T4292 9701-9705 NN denotes milk
T4293 9706-9708 IN denotes in
T4294 9709-9722 JJ denotes Tris-buffered
T4295 9723-9735 NNP denotes saline/Tween
T4296 9736-9738 CD denotes 20
T4297 9739-9740 -LRB- denotes (
T4298 9740-9747 NNP denotes Fischer
T4299 9748-9758 NNP denotes Scientific
T4300 9758-9759 , denotes ,
T4301 9760-9770 NNP denotes Pittsburgh
T4302 9770-9771 , denotes ,
T4303 9772-9774 NNP denotes PA
T4304 9774-9775 -RRB- denotes )
T4305 9776-9779 IN denotes for
T4306 9780-9781 CD denotes 1
T4307 9782-9786 NN denotes hour
T4308 9786-9787 . denotes .
T4309 9788-9799 JJ denotes Cytoplasmic
T4310 9800-9808 NNS denotes extracts
T4311 9809-9813 VBD denotes were
T4312 9814-9823 VBN denotes incubated
T4313 9824-9828 IN denotes with
T4314 9829-9843 JJ denotes phosphorylated
T4315 9844-9849 JJ denotes I-κBα
T4316 9850-9858 NN denotes antibody
T4317 9859-9860 -LRB- denotes (
T4318 9860-9865 CD denotes 1:200
T4319 9865-9866 -RRB- denotes )
T4320 9866-9867 , denotes ,
T4321 9868-9873 IN denotes while
T4322 9874-9881 JJ denotes nuclear
T4323 9882-9890 NNS denotes extracts
T4324 9891-9895 VBD denotes were
T4325 9896-9905 VBN denotes incubated
T4326 9906-9910 IN denotes with
T4327 9911-9917 DT denotes either
T4328 9918-9932 VBN denotes phosphorylated
T4329 9933-9938 NNP denotes NF-κB
T4330 9939-9942 CD denotes p65
T4331 9943-9951 NN denotes antibody
T4332 9952-9953 -LRB- denotes (
T4333 9953-9958 CD denotes 1:500
T4334 9958-9959 -RRB- denotes )
T4335 9960-9962 CC denotes or
T4336 9963-9977 VBN denotes phosphorylated
T4337 9978-9982 NNP denotes CREB
T4338 9983-9991 NN denotes antibody
T4339 9992-9993 -LRB- denotes (
T4340 9993-9998 CD denotes 1:500
T4341 9998-9999 -RRB- denotes )
T4342 10000-10009 RB denotes overnight
T4343 10010-10012 IN denotes at
T4344 10013-10014 CD denotes 4
T4345 10014-10015 CD denotes °
T4346 10015-10016 NNP denotes C
T4347 10017-10019 IN denotes in
T4348 10020-10028 JJ denotes separate
T4349 10029-10040 NNS denotes experiments
T4350 10040-10041 . denotes .
T4351 10042-10045 DT denotes The
T4352 10046-10055 NNS denotes membranes
T4353 10056-10060 VBD denotes were
T4354 10061-10067 VBN denotes washed
T4355 10068-10072 IN denotes with
T4356 10073-10086 JJ denotes Tris-buffered
T4357 10087-10099 NNP denotes saline/Tween
T4358 10100-10102 CD denotes 20
T4359 10103-10106 CC denotes and
T4360 10107-10116 VBD denotes incubated
T4361 10117-10120 IN denotes for
T4362 10121-10122 CD denotes 1
T4363 10123-10127 NN denotes hour
T4364 10128-10130 IN denotes at
T4365 10131-10135 NN denotes room
T4366 10136-10147 NN denotes temperature
T4367 10148-10152 IN denotes with
T4368 10153-10156 DT denotes the
T4369 10157-10166 JJ denotes secondary
T4370 10167-10175 NN denotes antibody
T4371 10175-10176 , denotes ,
T4372 10177-10188 JJ denotes horseradish
T4373 10189-10206 JJ denotes peroxidase-linked
T4374 10207-10218 JJ denotes anti-rabbit
T4375 10219-10222 NNP denotes IgG
T4376 10223-10230 VBD denotes diluted
T4377 10231-10237 CD denotes 1:2000
T4378 10238-10240 IN denotes in
T4379 10241-10249 VBG denotes blocking
T4380 10250-10258 NN denotes solution
T4381 10258-10259 . denotes .
T4382 10260-10265 IN denotes After
T4383 10266-10274 VBN denotes repeated
T4384 10275-10282 VBG denotes washing
T4385 10283-10285 IN denotes of
T4386 10286-10289 DT denotes the
T4387 10290-10298 NN denotes membrane
T4388 10298-10299 , denotes ,
T4389 10300-10303 DT denotes the
T4390 10304-10315 NNP denotes Supersignal
T4391 10316-10320 NNP denotes West
T4392 10321-10325 NNP denotes Pico
T4393 10326-10342 NNP denotes Chemiluminescent
T4394 10343-10346 NNP denotes Kit
T4395 10347-10350 VBD denotes was
T4396 10351-10358 VBN denotes applied
T4397 10359-10362 IN denotes for
T4398 10363-10371 NN denotes antibody
T4399 10372-10381 NN denotes detection
T4400 10382-10385 IN denotes per
T4401 10386-10389 DT denotes the
T4402 10390-10402 NN denotes manufacturer
T4403 10405-10417 NNS denotes instructions
T4404 10417-10418 . denotes .
T4405 10419-10426 JJ denotes Western
T4406 10427-10431 NN denotes blot
T4407 10432-10436 NNS denotes data
T4408 10437-10439 VBZ denotes is
T4409 10440-10449 VBN denotes presented
T4410 10450-10452 IN denotes as
T4411 10453-10454 DT denotes a
T4412 10455-10465 NN denotes percentage
T4413 10466-10468 IN denotes of
T4414 10469-10472 NNP denotes LPS
T4415 10473-10484 NN denotes stimulation
T4416 10484-10485 . denotes .
T4417 10486-10489 DT denotes The
T4418 10490-10500 NN denotes percentage
T4419 10501-10503 IN denotes of
T4420 10504-10507 NNP denotes LPS
T4421 10508-10519 NN denotes stimulation
T4422 10520-10523 VBD denotes was
T4423 10524-10534 VBN denotes calculated
T4424 10535-10537 IN denotes by
T4425 10538-10546 VBG denotes dividing
T4426 10547-10550 DT denotes the
T4427 10551-10555 JJ denotes mean
T4428 10556-10560 NN denotes band
T4429 10561-10566 NN denotes pixel
T4430 10567-10572 NN denotes total
T4431 10573-10576 IN denotes for
T4432 10577-10581 DT denotes each
T4433 10582-10591 NN denotes treatment
T4434 10592-10595 NN denotes arm
T4435 10596-10603 VBN denotes divided
T4436 10604-10606 IN denotes by
T4437 10607-10610 DT denotes the
T4438 10611-10615 JJ denotes mean
T4439 10616-10621 NN denotes pixel
T4440 10622-10627 NN denotes total
T4441 10628-10630 IN denotes of
T4442 10631-10638 NNS denotes samples
T4443 10639-10649 VBN denotes stimulated
T4444 10650-10654 IN denotes with
T4445 10655-10658 NNP denotes LPS
T4446 10659-10662 CC denotes and
T4447 10663-10674 VBG denotes multiplying
T4448 10675-10677 IN denotes by
T4449 10678-10681 CD denotes 100
T4450 10681-10682 . denotes .
T4451 10683-10687 RB denotes Thus
T4452 10687-10688 , denotes ,
T4453 10689-10692 NNP denotes LPS
T4454 10693-10704 NN denotes stimulation
T4455 10705-10707 VBZ denotes is
T4456 10708-10716 VBN denotes reported
T4457 10717-10719 IN denotes as
T4458 10720-10723 CD denotes 100
T4459 10723-10724 NN denotes %
T4460 10725-10728 CC denotes and
T4461 10729-10733 DT denotes each
T4462 10734-10736 IN denotes of
T4463 10737-10740 DT denotes the
T4464 10741-10750 NN denotes treatment
T4465 10751-10755 NNS denotes arms
T4466 10756-10758 VBZ denotes is
T4467 10759-10767 VBN denotes reported
T4468 10768-10770 IN denotes as
T4469 10771-10772 DT denotes a
T4470 10773-10780 NN denotes percent
T4471 10781-10783 IN denotes of
T4472 10784-10787 NNP denotes LPS
T4473 10788-10799 NN denotes stimulation
T4474 10799-10800 . denotes .
T4907 10802-10807 NN denotes NF-κB
T4908 10808-10811 CC denotes and
T4909 10812-10816 NNP denotes CREB
T4910 10817-10832 NNP denotes Electrophoretic
T4911 10833-10841 NNP denotes Mobility
T4912 10842-10847 NNP denotes Shift
T4913 10848-10853 NNP denotes Assay
T4914 10854-10855 -LRB- denotes (
T4915 10855-10859 NNP denotes EMSA
T4916 10859-10860 -RRB- denotes )
T4917 10861-10864 DT denotes The
T4918 10865-10880 JJ denotes non-radioactive
T4919 10881-10891 NNP denotes LightShift
T4920 10892-10908 NNP denotes Chemiluminescent
T4921 10909-10913 NNP denotes EMSA
T4922 10914-10917 NN denotes Kit
T4923 10918-10921 VBD denotes was
T4924 10922-10926 VBN denotes used
T4925 10927-10929 TO denotes to
T4926 10930-10936 VB denotes detect
T4927 10937-10948 JJ denotes DNA-protein
T4928 10949-10961 NNS denotes interactions
T4929 10961-10962 . denotes .
T4930 10963-10966 DT denotes The
T4931 10967-10972 NN denotes NF-κB
T4932 10973-10974 CD denotes 3
T4933 10974-10975 NN denotes ′
T4934 10976-10982 NN denotes biotin
T4935 10983-10994 VBD denotes end-labeled
T4936 10995-10998 NNP denotes DNA
T4937 10999-11014 NN denotes oligonucleotide
T4938 11015-11019 VBD denotes used
T4939 11020-11022 IN denotes as
T4940 11023-11024 DT denotes a
T4941 11025-11030 NN denotes probe
T4942 11031-11034 IN denotes for
T4943 11035-11038 DT denotes the
T4944 11039-11043 NNP denotes EMSA
T4945 11044-11047 VBD denotes was
T4946 11048-11049 DT denotes a
T4947 11050-11055 JJ denotes 42-bp
T4948 11056-11062 JJ denotes double
T4949 11063-11071 VBN denotes stranded
T4950 11072-11081 VBP denotes construct
T4951 11082-11083 -LRB- denotes (
T4952 11083-11084 CD denotes 5
T4953 11084-11085 SYM denotes ′
T4954 11085-11086 : denotes -
T4955 11086-11129 NN denotes TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
T4956 11132-11133 CD denotes 3
T4957 11133-11134 NN denotes ′
T4958 11134-11135 -RRB- denotes )
T4959 11136-11146 VBG denotes containing
T4960 11147-11150 CD denotes two
T4961 11151-11159 JJ denotes tandemly
T4962 11160-11168 VBN denotes repeated
T4963 11169-11174 NN denotes NF-κB
T4964 11175-11182 JJ denotes binding
T4965 11183-11188 NNS denotes sites
T4966 11189-11190 -LRB- denotes (
T4967 11190-11200 VBN denotes underlined
T4968 11200-11201 -RRB- denotes )
T4969 11201-11202 . denotes .
T4970 11203-11214 NN denotes Specificity
T4971 11215-11218 VBD denotes was
T4972 11219-11229 VBN denotes determined
T4973 11230-11232 IN denotes by
T4974 11233-11234 DT denotes a
T4975 11235-11246 NN denotes competition
T4976 11247-11252 NN denotes assay
T4977 11253-11257 IN denotes with
T4978 11258-11261 DT denotes the
T4979 11262-11270 NN denotes addition
T4980 11271-11273 IN denotes of
T4981 11274-11277 CD denotes 200
T4982 11278-11283 JJ denotes molar
T4983 11284-11290 NN denotes excess
T4984 11291-11293 IN denotes of
T4985 11294-11303 JJ denotes unlabeled
T4986 11304-11310 JJ denotes double
T4987 11311-11319 VBN denotes stranded
T4988 11320-11325 NN denotes NF-κB
T4989 11326-11341 NN denotes oligonucleotide
T4990 11341-11342 . denotes .
T4991 11343-11346 DT denotes The
T4992 11347-11351 NN denotes CREB
T4993 11352-11353 CD denotes 3
T4994 11353-11354 NN denotes ′
T4995 11355-11361 NN denotes biotin
T4996 11362-11373 VBD denotes end-labeled
T4997 11374-11377 NNP denotes DNA
T4998 11378-11393 NN denotes oligonucleotide
T4999 11394-11398 VBD denotes used
T5000 11399-11401 IN denotes as
T5001 11402-11403 DT denotes a
T5002 11404-11409 NN denotes probe
T5003 11410-11413 IN denotes for
T5004 11414-11417 DT denotes the
T5005 11418-11422 NNP denotes EMSA
T5006 11423-11426 VBD denotes was
T5007 11427-11428 DT denotes a
T5008 11429-11434 JJ denotes 23-bp
T5009 11435-11441 JJ denotes double
T5010 11442-11450 VBN denotes stranded
T5011 11451-11460 VBP denotes construct
T5012 11461-11462 -LRB- denotes (
T5013 11462-11463 CD denotes 5
T5014 11463-11464 SYM denotes ′
T5015 11464-11465 : denotes -
T5016 11465-11468 NNP denotes TTT
T5017 11469-11472 NNP denotes TCG
T5018 11473-11476 NNP denotes AGC
T5019 11477-11492 NNP denotes TCTGACGTCAGA-GC
T5020 11495-11496 CD denotes 3
T5021 11496-11497 NN denotes ′
T5022 11497-11498 -RRB- denotes )
T5023 11499-11509 VBG denotes containing
T5024 11510-11513 DT denotes the
T5025 11514-11517 NNP denotes CRE
T5026 11518-11527 NN denotes consensus
T5027 11528-11536 NN denotes sequence
T5028 11537-11538 -LRB- denotes (
T5029 11538-11548 VBN denotes underlined
T5030 11548-11549 -RRB- denotes )
T5031 11549-11550 . denotes .
T5032 11551-11562 NN denotes Specificity
T5033 11563-11566 VBD denotes was
T5034 11567-11577 VBN denotes determined
T5035 11578-11580 IN denotes by
T5036 11581-11582 DT denotes a
T5037 11583-11594 NN denotes competition
T5038 11595-11600 NN denotes assay
T5039 11601-11605 IN denotes with
T5040 11606-11609 DT denotes the
T5041 11610-11618 NN denotes addition
T5042 11619-11621 IN denotes of
T5043 11622-11625 CD denotes 200
T5044 11626-11631 JJ denotes molar
T5045 11632-11638 NN denotes excess
T5046 11639-11641 IN denotes of
T5047 11642-11651 JJ denotes unlabeled
T5048 11652-11658 JJ denotes double
T5049 11659-11667 VBN denotes stranded
T5050 11668-11672 NNP denotes CREB
T5051 11673-11688 NN denotes oligonucleotide
T5052 11688-11689 . denotes .
T5053 11690-11697 NNP denotes Nuclear
T5054 11698-11706 NNS denotes extracts
T5055 11707-11708 -LRB- denotes (
T5056 11708-11710 CD denotes 10
T5057 11711-11713 NN denotes μg
T5058 11713-11714 -RRB- denotes )
T5059 11715-11719 VBD denotes were
T5060 11720-11729 VBN denotes incubated
T5061 11730-11734 IN denotes with
T5062 11735-11736 CD denotes 5
T5063 11737-11739 NNP denotes nM
T5064 11740-11745 NNP denotes NF-κB
T5065 11746-11748 CC denotes or
T5066 11749-11753 NNP denotes CREB
T5067 11754-11759 NN denotes probe
T5068 11760-11761 -LRB- denotes (
T5069 11761-11766 NN denotes NF-kB
T5070 11766-11767 : denotes :
T5071 11768-11770 CD denotes 1x
T5072 11771-11778 JJ denotes binding
T5073 11779-11785 NN denotes buffer
T5074 11785-11786 , denotes ,
T5075 11787-11789 CD denotes 50
T5076 11790-11792 NNP denotes mM
T5077 11793-11796 NNP denotes KCl
T5078 11796-11797 , denotes ,
T5079 11798-11799 CD denotes 1
T5080 11800-11802 NNP denotes mM
T5081 11803-11807 NNP denotes EDTA
T5082 11807-11808 , denotes ,
T5083 11809-11810 CD denotes 1
T5084 11811-11813 NNP denotes mM
T5085 11814-11817 NNP denotes DTT
T5086 11817-11818 , denotes ,
T5087 11819-11822 CD denotes 0.1
T5088 11822-11823 NN denotes %
T5089 11824-11828 CD denotes NP40
T5090 11828-11829 , denotes ,
T5091 11830-11832 CD denotes 10
T5092 11832-11833 NN denotes %
T5093 11834-11842 NN denotes glycerol
T5094 11842-11843 , denotes ,
T5095 11844-11847 CC denotes and
T5096 11848-11850 CD denotes 50
T5097 11851-11853 NN denotes ng
T5098 11853-11854 NN denotes /
T5099 11854-11856 NN denotes μl
T5100 11857-11861 NN denotes poly
T5101 11862-11867 NN denotes dI-dC
T5102 11867-11868 , denotes ,
T5103 11869-11873 NNP denotes CREB
T5104 11873-11874 : denotes :
T5105 11875-11877 JJ denotes 1x
T5106 11878-11885 JJ denotes binding
T5107 11886-11892 NN denotes buffer
T5108 11892-11893 , denotes ,
T5109 11894-11896 CD denotes 20
T5110 11897-11899 NNP denotes mM
T5111 11900-11904 NNP denotes Tris
T5112 11904-11905 , denotes ,
T5113 11906-11908 NNP denotes pH
T5114 11909-11912 CD denotes 7.5
T5115 11912-11913 , denotes ,
T5116 11914-11916 CD denotes 50
T5117 11917-11919 NNP denotes mM
T5118 11920-11923 NNP denotes KCl
T5119 11923-11924 , denotes ,
T5120 11925-11926 CD denotes 1
T5121 11927-11929 NNP denotes mM
T5122 11930-11934 NNP denotes EDTA
T5123 11934-11935 , denotes ,
T5124 11936-11937 CD denotes 1
T5125 11938-11940 NNP denotes mM
T5126 11941-11944 NNP denotes DTT
T5127 11944-11945 , denotes ,
T5128 11946-11950 CD denotes 0.10
T5129 11950-11951 NN denotes %
T5130 11952-11956 CD denotes NP40
T5131 11956-11957 , denotes ,
T5132 11958-11959 CD denotes 6
T5133 11959-11960 NN denotes %
T5134 11961-11969 NN denotes glycerol
T5135 11969-11970 , denotes ,
T5136 11971-11974 CD denotes 0.1
T5137 11975-11980 NNP denotes mg/mL
T5138 11981-11984 NNP denotes BSA
T5139 11985-11988 CC denotes and
T5140 11989-11991 CD denotes 50
T5141 11992-11994 NN denotes ng
T5142 11994-11995 NN denotes /
T5143 11995-11997 NN denotes μl
T5144 11998-12002 NN denotes poly
T5145 12003-12008 NN denotes dI-dC
T5146 12008-12009 -RRB- denotes )
T5147 12010-12013 CC denotes and
T5148 12014-12018 VBD denotes were
T5149 12019-12023 RB denotes then
T5150 12024-12039 VBN denotes electrophoresed
T5151 12040-12047 IN denotes through
T5152 12048-12049 DT denotes a
T5153 12050-12051 CD denotes 6
T5154 12051-12052 NN denotes %
T5155 12053-12056 NN denotes DNA
T5156 12057-12068 NN denotes retardation
T5157 12069-12072 NN denotes gel
T5158 12073-12075 IN denotes at
T5159 12076-12080 CD denotes 100V
T5160 12081-12084 IN denotes for
T5161 12085-12087 CD denotes 90
T5162 12088-12095 NNS denotes minutes
T5163 12095-12096 . denotes .
T5164 12097-12100 DT denotes The
T5165 12101-12105 NNS denotes gels
T5166 12106-12110 VBD denotes were
T5167 12111-12130 RB denotes electrophoretically
T5168 12131-12142 VBN denotes transferred
T5169 12143-12145 IN denotes at
T5170 12146-12151 NNP denotes 380mA
T5171 12152-12155 IN denotes for
T5172 12156-12157 CD denotes 1
T5173 12158-12162 NN denotes hour
T5174 12163-12165 IN denotes on
T5175 12166-12169 NN denotes ice
T5176 12170-12172 TO denotes to
T5177 12173-12174 DT denotes a
T5178 12175-12185 RB denotes positively
T5179 12186-12193 VBN denotes charged
T5180 12194-12199 NN denotes nylon
T5181 12200-12208 NN denotes membrane
T5182 12209-12212 CC denotes and
T5183 12213-12224 RB denotes immediately
T5184 12225-12237 JJ denotes cross-linked
T5185 12238-12241 IN denotes for
T5186 12242-12244 CD denotes 15
T5187 12245-12252 NNS denotes minutes
T5188 12253-12257 IN denotes with
T5189 12258-12259 DT denotes a
T5190 12260-12262 NNP denotes UV
T5191 12263-12279 NN denotes transilluminator
T5192 12280-12288 VBN denotes equipped
T5193 12289-12293 IN denotes with
T5194 12294-12295 DT denotes a
T5195 12296-12299 CD denotes 312
T5196 12300-12302 NN denotes nm
T5197 12303-12307 NN denotes bulb
T5198 12307-12308 . denotes .
T5199 12309-12333 JJ denotes Streptavidin-horseradish
T5200 12334-12344 NN denotes peroxidase
T5201 12345-12354 NN denotes conjugate
T5202 12355-12358 CC denotes and
T5203 12359-12362 DT denotes the
T5204 12363-12373 NNP denotes LightShift
T5205 12374-12390 NNP denotes Chemiluminescent
T5206 12391-12400 NNP denotes Substrate
T5207 12401-12405 VBD denotes were
T5208 12406-12410 VBN denotes used
T5209 12411-12413 TO denotes to
T5210 12414-12420 VB denotes detect
T5211 12421-12424 DT denotes the
T5212 12425-12431 NN denotes biotin
T5213 12432-12443 VBD denotes end-labeled
T5214 12444-12447 NNP denotes DNA
T5215 12447-12448 . denotes .
T5216 12449-12452 DT denotes The
T5217 12453-12458 NN denotes nylon
T5218 12459-12468 NNS denotes membranes
T5219 12469-12473 VBD denotes were
T5220 12474-12481 VBN denotes exposed
T5221 12482-12484 TO denotes to
T5222 12485-12490 VB denotes x-ray
T5223 12491-12495 NN denotes film
T5224 12496-12499 IN denotes for
T5225 12500-12501 CD denotes 1
T5226 12502-12503 CD denotes 3
T5227 12504-12511 NNS denotes minutes
T5228 12512-12515 IN denotes for
T5229 12516-12525 NN denotes detection
T5230 12525-12526 . denotes .
T5650 12528-12532 NN denotes Role
T5651 12533-12535 IN denotes of
T5652 12536-12539 NNP denotes PKA
T5653 12540-12542 IN denotes on
T5654 12543-12554 JJ denotes LPS-induced
T5655 12555-12566 NN denotes mononuclear
T5656 12567-12571 NN denotes cell
T5657 12572-12577 JJ denotes TNF-α
T5658 12578-12588 NN denotes production
T5659 12589-12591 TO denotes To
T5660 12592-12600 VB denotes evaluate
T5661 12601-12604 DT denotes the
T5662 12605-12609 NN denotes role
T5663 12610-12612 IN denotes of
T5664 12613-12616 NNP denotes PKA
T5665 12617-12619 IN denotes on
T5666 12620-12625 JJ denotes TNF-α
T5667 12626-12636 NN denotes production
T5668 12637-12639 IN denotes in
T5669 12640-12651 NN denotes mononuclear
T5670 12652-12657 NNS denotes cells
T5671 12658-12661 CC denotes and
T5672 12662-12665 PRP$ denotes its
T5673 12666-12677 NN denotes involvement
T5674 12678-12680 IN denotes in
T5675 12681-12684 DT denotes the
T5676 12685-12696 NN denotes attenuation
T5677 12697-12699 IN denotes of
T5678 12700-12711 JJ denotes LPS-induced
T5679 12712-12717 JJ denotes TNF-α
T5680 12718-12728 NN denotes production
T5681 12729-12737 VBD denotes observed
T5682 12738-12747 VBG denotes following
T5683 12748-12751 NNP denotes PTX
T5684 12752-12761 NN denotes treatment
T5685 12761-12762 , denotes ,
T5686 12763-12766 NNP denotes H89
T5687 12766-12767 , denotes ,
T5688 12768-12769 DT denotes a
T5689 12770-12778 JJ denotes specific
T5690 12779-12782 NNP denotes PKA
T5691 12783-12792 NN denotes inhibitor
T5692 12792-12793 , denotes ,
T5693 12794-12797 VBD denotes was
T5694 12798-12806 VBN denotes utilized
T5695 12806-12807 . denotes .
T5696 12808-12816 VBN denotes Isolated
T5697 12817-12828 NN denotes mononuclear
T5698 12829-12834 NNS denotes cells
T5699 12835-12839 VBD denotes were
T5700 12840-12849 VBN denotes incubated
T5701 12850-12859 VBG denotes according
T5702 12860-12862 TO denotes to
T5703 12863-12866 DT denotes the
T5704 12867-12876 NN denotes treatment
T5705 12877-12883 NNS denotes groups
T5706 12884-12893 VBN denotes described
T5707 12894-12899 IN denotes above
T5708 12900-12902 IN denotes in
T5709 12903-12906 DT denotes the
T5710 12907-12915 NN denotes presence
T5711 12916-12919 CC denotes and
T5712 12920-12927 NN denotes absence
T5713 12928-12930 IN denotes of
T5714 12931-12943 NN denotes pretreatment
T5715 12944-12948 IN denotes with
T5716 12949-12952 NNP denotes H89
T5717 12953-12954 -LRB- denotes (
T5718 12954-12956 CD denotes 10
T5719 12957-12959 NNP denotes μM
T5720 12959-12960 -RRB- denotes )
T5721 12961-12964 IN denotes for
T5722 12965-12966 CD denotes 1
T5723 12967-12971 NN denotes hour
T5724 12972-12974 IN denotes at
T5725 12975-12979 NNP denotes 37ºC
T5726 12979-12980 . denotes .
T5727 12981-12984 DT denotes The
T5728 12985-12989 NN denotes dose
T5729 12990-12992 IN denotes of
T5730 12993-12996 NNP denotes H89
T5731 12997-13000 VBD denotes was
T5732 13001-13007 VBN denotes chosen
T5733 13008-13013 VBN denotes based
T5734 13014-13016 IN denotes on
T5735 13017-13025 JJ denotes previous
T5736 13026-13030 NN denotes work
T5737 13031-13036 WDT denotes which
T5738 13037-13049 VBD denotes demonstrated
T5739 13050-13058 JJ denotes specific
T5740 13059-13062 CC denotes and
T5741 13063-13071 JJ denotes complete
T5742 13072-13082 NN denotes inhibition
T5743 13083-13085 IN denotes of
T5744 13086-13089 NNP denotes PKA
T5745 13090-13092 IN denotes at
T5746 13093-13097 DT denotes this
T5747 13098-13111 NN denotes concentration
T5748 13111-13114 CD denotes .10
T5749 13115-13120 IN denotes Since
T5750 13121-13124 DT denotes the
T5751 13125-13133 NN denotes activity
T5752 13134-13136 IN denotes of
T5753 13137-13141 DT denotes this
T5754 13142-13151 NN denotes inhibitor
T5755 13152-13154 VBZ denotes is
T5756 13155-13159 DT denotes both
T5757 13160-13168 JJ denotes specific
T5758 13169-13172 CC denotes and
T5759 13173-13183 JJ denotes consistent
T5760 13184-13186 IN denotes at
T5761 13187-13191 DT denotes this
T5762 13192-13201 VBN denotes specified
T5763 13202-13206 NN denotes dose
T5764 13206-13207 , denotes ,
T5765 13208-13210 PRP denotes we
T5766 13211-13214 VBD denotes did
T5767 13215-13218 RB denotes not
T5768 13219-13226 VB denotes include
T5769 13227-13238 NNS denotes experiments
T5770 13239-13243 IN denotes with
T5771 13244-13254 JJ denotes additional
T5772 13255-13258 NNP denotes PKA
T5773 13259-13269 NNS denotes inhibitors
T5774 13270-13274 IN denotes with
T5775 13275-13279 DT denotes this
T5776 13280-13283 NN denotes set
T5777 13284-13286 IN denotes of
T5778 13287-13298 NNS denotes experiments
T5779 13298-13299 . denotes .
T5780 13300-13303 DT denotes The
T5781 13304-13309 JJ denotes TNF-α
T5782 13310-13323 NN denotes concentration
T5783 13324-13326 IN denotes in
T5784 13327-13330 DT denotes the
T5785 13331-13342 NN denotes supernatant
T5786 13343-13346 VBD denotes was
T5787 13347-13355 VBN denotes measured
T5788 13356-13370 RB denotes quantitatively
T5789 13371-13373 IN denotes by
T5790 13374-13379 NNP denotes ELISA
T5791 13379-13380 . denotes .
T5792 13381-13384 DT denotes The
T5793 13385-13392 NNS denotes results
T5794 13393-13396 VBP denotes are
T5795 13397-13406 VBN denotes expressed
T5796 13407-13409 IN denotes in
T5797 13410-13415 NNP denotes pg/mL
T5798 13415-13416 . denotes .
T6072 13418-13429 NNP denotes Statistical
T6073 13430-13438 NNP denotes Analysis
T6074 13439-13442 NNP denotes The
T6075 13443-13455 JJ denotes experimental
T6076 13456-13463 NNS denotes results
T6077 13464-13472 VBN denotes obtained
T6078 13473-13475 IN denotes in
T6079 13476-13480 DT denotes this
T6080 13481-13486 NN denotes study
T6081 13487-13491 VBD denotes were
T6082 13492-13499 VBN denotes derived
T6083 13500-13504 IN denotes from
T6084 13505-13509 CD denotes four
T6085 13510-13518 JJ denotes separate
T6086 13519-13530 NNS denotes experiments
T6087 13531-13535 IN denotes with
T6088 13536-13543 JJ denotes healthy
T6089 13544-13553 NN denotes volunteer
T6090 13554-13560 NNS denotes donors
T6091 13560-13561 . denotes .
T6092 13562-13566 DT denotes Each
T6093 13567-13572 NN denotes assay
T6094 13573-13576 VBD denotes was
T6095 13577-13586 VBN denotes performed
T6096 13587-13589 IN denotes in
T6097 13590-13599 VBP denotes duplicate
T6098 13600-13602 CC denotes or
T6099 13603-13613 VBP denotes triplicate
T6100 13614-13619 WRB denotes where
T6101 13620-13631 JJ denotes appropriate
T6102 13631-13632 . denotes .
T6103 13633-13637 NNP denotes Data
T6104 13638-13640 VBZ denotes is
T6105 13641-13650 VBN denotes presented
T6106 13651-13653 IN denotes as
T6107 13654-13657 DT denotes the
T6108 13658-13662 JJ denotes mean
T6109 13663-13664 NN denotes ±
T6110 13665-13668 NNP denotes SEM
T6111 13668-13669 . denotes .
T6112 13670-13681 NNP denotes Statistical
T6113 13682-13693 NNS denotes differences
T6114 13694-13701 IN denotes between
T6115 13702-13708 NNS denotes groups
T6116 13709-13713 VBD denotes were
T6117 13714-13724 VBN denotes determined
T6118 13725-13727 IN denotes by
T6119 13728-13735 JJ denotes one-way
T6120 13736-13744 NN denotes analysis
T6121 13745-13747 IN denotes of
T6122 13748-13756 NN denotes variance
T6123 13757-13758 -LRB- denotes (
T6124 13758-13763 NNP denotes ANOVA
T6125 13763-13764 -RRB- denotes )
T6126 13765-13769 IN denotes with
T6127 13770-13771 DT denotes a
T6128 13772-13782 NNP denotes Bonferroni
T6129 13783-13793 NN denotes correction
T6130 13793-13794 . denotes .
T6131 13795-13806 NNP denotes Statistical
T6132 13807-13819 NN denotes significance
T6133 13820-13823 VBD denotes was
T6134 13824-13831 VBN denotes defined
T6135 13832-13834 IN denotes as
T6136 13835-13836 NNP denotes P
T6137 13837-13838 CD denotes <
T6138 13839-13843 CD denotes 0.05
T6139 13843-13844 . denotes .
T6242 13855-13858 DT denotes The
T6243 13859-13866 NNS denotes effects
T6244 13867-13869 IN denotes of
T6245 13870-13873 NNP denotes PTX
T6246 13874-13876 IN denotes on
T6247 13877-13888 JJ denotes LPS-induced
T6248 13889-13894 JJ denotes TNF-α
T6249 13895-13905 NN denotes production
T6250 13906-13908 TO denotes To
T6251 13909-13918 VB denotes determine
T6252 13919-13922 DT denotes the
T6253 13923-13931 NN denotes duration
T6254 13932-13934 IN denotes of
T6255 13935-13938 NNP denotes PDE
T6256 13939-13949 NN denotes inhibition
T6257 13950-13952 IN denotes on
T6258 13953-13958 JJ denotes TNF-α
T6259 13959-13969 NN denotes production
T6260 13970-13975 IN denotes after
T6261 13976-13979 NNP denotes LPS
T6262 13980-13991 NN denotes stimulation
T6263 13991-13992 , denotes ,
T6264 13993-13997 NN denotes time
T6265 13998-14004 NN denotes course
T6266 14005-14012 NNS denotes studies
T6267 14013-14017 VBD denotes were
T6268 14018-14027 VBN denotes conducted
T6269 14027-14028 . denotes .
T6270 14029-14031 IN denotes In
T6271 14032-14042 NN denotes accordance
T6272 14043-14047 IN denotes with
T6273 14048-14053 JJ denotes prior
T6274 14054-14058 NN denotes work
T6275 14058-14059 , denotes ,
T6276 14060-14063 NNP denotes PTX
T6277 14064-14067 VBD denotes had
T6278 14068-14069 DT denotes a
T6279 14070-14075 JJ denotes rapid
T6280 14076-14079 CC denotes and
T6281 14080-14089 VBD denotes sustained
T6282 14090-14096 NN denotes effect
T6283 14097-14099 IN denotes on
T6284 14100-14105 JJ denotes TNF-α
T6285 14106-14116 NN denotes production
T6286 14117-14119 IN denotes in
T6287 14120-14125 NN denotes vitro
T6288 14126-14127 -LRB- denotes (
T6289 14127-14133 NN denotes Figure
T6290 14134-14135 CD denotes 1
T6291 14135-14136 -RRB- denotes )
T6292 14136-14137 . denotes .
T6505 14139-14142 NNP denotes PTX
T6506 14143-14152 VBZ denotes decreases
T6507 14153-14164 JJ denotes LPS-induced
T6508 14165-14170 NN denotes NF-κB
T6509 14171-14181 NN denotes activation
T6510 14182-14187 IN denotes Since
T6511 14188-14203 NN denotes phosphorylation
T6512 14204-14209 VBZ denotes leads
T6513 14210-14212 TO denotes to
T6514 14213-14227 NN denotes ubiquitination
T6515 14228-14231 CC denotes and
T6516 14232-14243 NN denotes degradation
T6517 14244-14246 IN denotes of
T6518 14247-14252 NNP denotes I-κBα
T6519 14253-14256 CC denotes and
T6520 14257-14267 JJ denotes subsequent
T6521 14268-14275 JJ denotes nuclear
T6522 14276-14289 NN denotes translocation
T6523 14290-14292 IN denotes of
T6524 14293-14298 NNP denotes NF-κB
T6525 14298-14299 , denotes ,
T6526 14300-14302 PRP denotes we
T6527 14303-14308 RB denotes first
T6528 14309-14317 VBD denotes examined
T6529 14318-14321 DT denotes the
T6530 14322-14329 NNS denotes effects
T6531 14330-14332 IN denotes of
T6532 14333-14336 NNP denotes PTX
T6533 14337-14339 IN denotes on
T6534 14340-14345 NNP denotes I-κBα
T6535 14345-14346 . denotes .
T6536 14347-14358 NNP denotes Cytoplasmic
T6537 14359-14364 NNP denotes I-κBα
T6538 14365-14380 NN denotes phosphorylation
T6539 14381-14384 VBD denotes was
T6540 14385-14393 RB denotes markedly
T6541 14394-14403 VBN denotes increased
T6542 14404-14413 VBG denotes following
T6543 14414-14417 NNP denotes LPS
T6544 14418-14429 NN denotes stimulation
T6545 14430-14434 WRB denotes when
T6546 14435-14443 VBN denotes compared
T6547 14444-14446 TO denotes to
T6548 14447-14454 VB denotes control
T6549 14455-14456 -LRB- denotes (
T6550 14456-14459 CD denotes 100
T6551 14460-14461 CD denotes ±
T6552 14462-14463 CD denotes 0
T6553 14464-14467 IN denotes vs.
T6554 14468-14470 CD denotes 20
T6555 14471-14472 CD denotes ±
T6556 14473-14475 CD denotes 18
T6557 14475-14476 : denotes ;
T6558 14477-14478 NNP denotes P
T6559 14479-14480 NNP denotes <
T6560 14481-14485 CD denotes 0.05
T6561 14485-14486 -RRB- denotes )
T6562 14486-14487 . denotes .
T6563 14488-14491 DT denotes The
T6564 14492-14500 NN denotes addition
T6565 14501-14503 IN denotes of
T6566 14504-14507 NNP denotes PTX
T6567 14508-14521 RB denotes significantly
T6568 14522-14535 VBD denotes downregulated
T6569 14536-14547 JJ denotes LPS-induced
T6570 14548-14553 JJ denotes I-κBα
T6571 14554-14569 NN denotes phosphorylation
T6572 14570-14571 -LRB- denotes (
T6573 14571-14572 NNP denotes P
T6574 14573-14574 SYM denotes =
T6575 14575-14579 CD denotes 0.02
T6576 14579-14580 : denotes ;
T6577 14581-14587 NN denotes Figure
T6578 14588-14589 CD denotes 2
T6579 14589-14590 -RRB- denotes )
T6580 14591-14593 IN denotes In
T6581 14594-14595 DT denotes a
T6582 14596-14603 JJ denotes similar
T6583 14604-14611 NN denotes fashion
T6584 14611-14612 , denotes ,
T6585 14613-14620 JJ denotes nuclear
T6586 14621-14626 NNP denotes NF-κB
T6587 14627-14630 CD denotes p65
T6588 14631-14646 NN denotes phosphorylation
T6589 14647-14650 VBD denotes was
T6590 14651-14660 VBN denotes increased
T6591 14661-14670 VBG denotes following
T6592 14671-14674 NNP denotes LPS
T6593 14675-14686 NN denotes stimulation
T6594 14687-14688 -LRB- denotes (
T6595 14688-14691 CD denotes 100
T6596 14692-14693 CD denotes ±
T6597 14694-14695 CD denotes 0
T6598 14696-14699 IN denotes vs.
T6599 14700-14702 CD denotes 38
T6600 14703-14704 CD denotes ±
T6601 14705-14707 CD denotes 20
T6602 14707-14708 : denotes ;
T6603 14709-14710 NNP denotes P
T6604 14711-14712 NNP denotes <
T6605 14713-14717 CD denotes 0.01
T6606 14717-14718 -RRB- denotes )
T6607 14718-14719 . denotes .
T6608 14720-14723 NNP denotes PTX
T6609 14724-14733 RB denotes similarly
T6610 14734-14743 VBD denotes decreased
T6611 14744-14755 JJ denotes LPS-induced
T6612 14756-14761 NNP denotes NF-κB
T6613 14762-14765 CD denotes p65
T6614 14766-14773 JJ denotes nuclear
T6615 14774-14789 NN denotes phosphorylation
T6616 14789-14790 , denotes ,
T6617 14791-14792 DT denotes a
T6618 14793-14799 NN denotes marker
T6619 14800-14802 IN denotes of
T6620 14803-14808 JJ denotes NF-κB
T6621 14809-14819 NN denotes activation
T6622 14820-14823 CC denotes and
T6623 14824-14831 JJ denotes nuclear
T6624 14832-14845 NN denotes translocation
T6625 14846-14847 -LRB- denotes (
T6626 14847-14850 CD denotes 100
T6627 14851-14852 CD denotes ±
T6628 14853-14854 CD denotes 0
T6629 14855-14858 IN denotes vs.
T6630 14859-14861 CD denotes 40
T6631 14862-14863 NN denotes ±
T6632 14864-14865 CD denotes 6
T6633 14865-14866 : denotes ;
T6634 14867-14868 NNP denotes P
T6635 14869-14870 SYM denotes =
T6636 14870-14874 CD denotes 0.03
T6637 14874-14875 : denotes ;
T6638 14876-14882 NNP denotes Figure
T6639 14883-14885 NNP denotes 3A
T6640 14885-14886 -RRB- denotes )
T6641 14886-14887 . denotes .
T6642 14888-14893 NNS denotes EMSAs
T6643 14894-14898 VBD denotes were
T6644 14899-14903 RB denotes then
T6645 14904-14913 VBN denotes performed
T6646 14914-14916 TO denotes to
T6647 14917-14923 VB denotes verify
T6648 14924-14927 DT denotes the
T6649 14928-14939 JJ denotes PTX-induced
T6650 14940-14951 NNS denotes alterations
T6651 14952-14954 IN denotes in
T6652 14955-14962 JJ denotes nuclear
T6653 14963-14978 NN denotes phosphorylation
T6654 14979-14987 VBD denotes resulted
T6655 14988-14990 IN denotes in
T6656 14991-14998 JJ denotes similar
T6657 14999-15006 NNS denotes effects
T6658 15007-15009 IN denotes on
T6659 15010-15013 DT denotes the
T6660 15014-15017 NNP denotes DNA
T6661 15018-15025 JJ denotes binding
T6662 15026-15034 NN denotes activity
T6663 15035-15037 IN denotes of
T6664 15038-15043 NN denotes NF-κB
T6665 15043-15044 . denotes .
T6666 15045-15048 DT denotes The
T6667 15049-15057 NN denotes addition
T6668 15058-15060 IN denotes of
T6669 15061-15064 NNP denotes PTX
T6670 15065-15067 TO denotes to
T6671 15068-15082 JJ denotes LPS-stimulated
T6672 15083-15094 NN denotes mononuclear
T6673 15095-15100 NNS denotes cells
T6674 15101-15109 VBD denotes resulted
T6675 15110-15112 IN denotes in
T6676 15113-15123 JJ denotes comparable
T6677 15124-15138 NN denotes downregulation
T6678 15139-15141 IN denotes of
T6679 15142-15145 NNP denotes DNA
T6680 15146-15153 JJ denotes binding
T6681 15154-15162 NN denotes activity
T6682 15162-15163 , denotes ,
T6683 15164-15171 JJ denotes similar
T6684 15172-15174 TO denotes to
T6685 15175-15178 DT denotes the
T6686 15179-15187 JJ denotes observed
T6687 15188-15202 NN denotes downregulation
T6688 15203-15205 IN denotes of
T6689 15206-15211 JJ denotes NF-κB
T6690 15212-15227 NN denotes phosphorylation
T6691 15228-15229 -LRB- denotes (
T6692 15229-15235 NN denotes Figure
T6693 15236-15238 NN denotes 3B
T6694 15238-15239 -RRB- denotes )
T6695 15239-15240 . denotes .
T7091 15242-15245 NNP denotes PTX
T7092 15246-15257 VBZ denotes upregulates
T7093 15258-15262 NNP denotes CREB
T7094 15263-15278 NN denotes phosphorylation
T7095 15279-15282 CC denotes and
T7096 15283-15293 NN denotes activation
T7097 15294-15299 IN denotes after
T7098 15300-15303 NNP denotes LPS
T7099 15304-15315 NN denotes stimulation
T7100 15316-15331 NNP denotes Phosphorylation
T7101 15332-15334 IN denotes of
T7102 15335-15342 JJ denotes nuclear
T7103 15343-15347 NNP denotes CREB
T7104 15348-15351 VBD denotes was
T7105 15352-15356 VBN denotes used
T7106 15357-15359 IN denotes as
T7107 15360-15361 DT denotes a
T7108 15362-15368 NN denotes marker
T7109 15369-15372 IN denotes for
T7110 15373-15377 NNP denotes CREB
T7111 15378-15388 NN denotes activation
T7112 15388-15389 . denotes .
T7113 15390-15393 NNP denotes LPS
T7114 15394-15405 NN denotes stimulation
T7115 15406-15412 VBD denotes caused
T7116 15413-15414 DT denotes a
T7117 15415-15425 JJ denotes negligible
T7118 15426-15434 NN denotes increase
T7119 15435-15437 IN denotes in
T7120 15438-15442 NNP denotes CREB
T7121 15443-15458 NN denotes phosphorylation
T7122 15459-15463 WRB denotes when
T7123 15464-15472 VBN denotes compared
T7124 15473-15475 TO denotes to
T7125 15476-15483 VB denotes control
T7126 15484-15485 -LRB- denotes (
T7127 15485-15491 NN denotes Figure
T7128 15492-15494 NN denotes 4A
T7129 15494-15495 -RRB- denotes )
T7130 15495-15496 . denotes .
T7131 15497-15500 NNP denotes PTX
T7132 15501-15506 RB denotes alone
T7133 15507-15513 VBD denotes caused
T7134 15514-15515 DT denotes a
T7135 15516-15522 JJ denotes marked
T7136 15523-15531 NN denotes increase
T7137 15532-15534 IN denotes in
T7138 15535-15539 NNP denotes CREB
T7139 15540-15555 NN denotes phosphorylation
T7140 15556-15557 -LRB- denotes (
T7141 15557-15558 NNP denotes P
T7142 15559-15560 CD denotes <
T7143 15561-15565 CD denotes 0.01
T7144 15566-15569 IN denotes vs.
T7145 15570-15574 NNP denotes HBSS
T7146 15574-15575 -RRB- denotes )
T7147 15575-15576 . denotes .
T7148 15577-15581 WRB denotes When
T7149 15582-15585 NNP denotes LPS
T7150 15586-15589 CC denotes and
T7151 15590-15593 NNP denotes PTX
T7152 15594-15602 NN denotes exposure
T7153 15603-15611 VBD denotes occurred
T7154 15612-15626 RB denotes simultaneously
T7155 15626-15627 , denotes ,
T7156 15628-15632 NNP denotes CREB
T7157 15633-15648 NN denotes phosphorylation
T7158 15649-15652 VBD denotes was
T7159 15653-15666 RB denotes significantly
T7160 15667-15673 JJR denotes higher
T7161 15674-15678 IN denotes than
T7162 15679-15683 IN denotes with
T7163 15684-15687 NNP denotes LPS
T7164 15688-15699 NN denotes stimulation
T7165 15700-15705 RB denotes alone
T7166 15706-15707 -LRB- denotes (
T7167 15707-15710 CD denotes 543
T7168 15711-15712 CD denotes ±
T7169 15713-15715 CD denotes 92
T7170 15716-15719 IN denotes vs.
T7171 15720-15723 CD denotes 100
T7172 15724-15725 CD denotes ±
T7173 15726-15727 CD denotes 0
T7174 15727-15728 : denotes ;
T7175 15729-15730 NNP denotes P
T7176 15731-15732 NNP denotes <
T7177 15733-15737 CD denotes 0.01
T7178 15737-15738 -RRB- denotes )
T7179 15738-15739 . denotes .
T7180 15740-15742 IN denotes In
T7181 15743-15751 NN denotes addition
T7182 15751-15752 , denotes ,
T7183 15753-15756 DT denotes the
T7184 15757-15763 NN denotes amount
T7185 15764-15766 IN denotes of
T7186 15767-15771 NNP denotes CREB
T7187 15772-15787 NN denotes phosphorylation
T7188 15788-15792 VBN denotes seen
T7189 15793-15797 IN denotes with
T7190 15798-15809 JJ denotes concomitant
T7191 15810-15813 NNP denotes LPS
T7192 15814-15817 CC denotes and
T7193 15818-15821 NNP denotes PTX
T7194 15822-15831 NN denotes treatment
T7195 15832-15835 VBD denotes was
T7196 15836-15840 JJR denotes less
T7197 15841-15845 IN denotes than
T7198 15846-15850 DT denotes that
T7199 15851-15855 VBN denotes seen
T7200 15856-15860 IN denotes with
T7201 15861-15864 NNP denotes PTX
T7202 15865-15870 RB denotes alone
T7203 15870-15871 , denotes ,
T7204 15872-15880 IN denotes although
T7205 15881-15885 DT denotes this
T7206 15886-15896 NN denotes difference
T7207 15897-15900 VBD denotes was
T7208 15901-15904 RB denotes not
T7209 15905-15918 RB denotes statistically
T7210 15919-15930 JJ denotes significant
T7211 15931-15932 -LRB- denotes (
T7212 15932-15933 NNP denotes P
T7213 15934-15935 SYM denotes =
T7214 15936-15940 CD denotes 0.08
T7215 15940-15941 -RRB- denotes )
T7216 15941-15942 . denotes .
T7217 15943-15945 TO denotes To
T7218 15946-15955 VB denotes determine
T7219 15956-15958 IN denotes if
T7220 15959-15967 NNP denotes CREB-DNA
T7221 15968-15975 JJ denotes binding
T7222 15976-15979 VBD denotes was
T7223 15980-15988 VBN denotes affected
T7224 15989-15991 IN denotes by
T7225 15992-15995 NNP denotes PTX
T7226 15996-15998 IN denotes in
T7227 15999-16000 DT denotes a
T7228 16001-16007 NN denotes manner
T7229 16008-16015 JJ denotes similar
T7230 16016-16018 TO denotes to
T7231 16019-16023 NNP denotes CREB
T7232 16024-16039 NN denotes phosphorylation
T7233 16039-16040 , denotes ,
T7234 16041-16043 DT denotes an
T7235 16044-16048 NNP denotes EMSA
T7236 16049-16052 VBD denotes was
T7237 16053-16062 VBN denotes performed
T7238 16062-16063 . denotes .
T7239 16064-16072 NN denotes Exposure
T7240 16073-16075 IN denotes of
T7241 16076-16090 JJ denotes LPS-stimulated
T7242 16091-16096 NNS denotes cells
T7243 16097-16099 TO denotes to
T7244 16100-16103 NNP denotes PTX
T7245 16104-16113 RB denotes similarly
T7246 16114-16123 VBD denotes increased
T7247 16124-16128 NNP denotes CREB
T7248 16129-16140 JJ denotes DNA-binding
T7249 16141-16149 VBN denotes compared
T7250 16150-16152 TO denotes to
T7251 16153-16156 NNP denotes LPS
T7252 16157-16162 RB denotes alone
T7253 16163-16164 -LRB- denotes (
T7254 16164-16170 NN denotes Figure
T7255 16171-16173 NN denotes 4B
T7256 16173-16174 -RRB- denotes )
T7257 16174-16175 . denotes .
T7611 16177-16183 NN denotes Effect
T7612 16184-16186 IN denotes of
T7613 16187-16190 NNP denotes PTX
T7614 16191-16193 IN denotes on
T7615 16194-16199 JJ denotes TNF-α
T7616 16200-16210 NN denotes production
T7617 16211-16213 IN denotes in
T7618 16214-16217 DT denotes the
T7619 16218-16226 NN denotes presence
T7620 16227-16229 IN denotes of
T7621 16230-16233 NNP denotes PKA
T7622 16234-16244 NN denotes inhibition
T7623 16245-16248 DT denotes The
T7624 16249-16253 NN denotes role
T7625 16254-16256 IN denotes of
T7626 16257-16260 NNP denotes PKA
T7627 16261-16263 IN denotes in
T7628 16264-16275 JJ denotes PTX-induced
T7629 16276-16290 NN denotes downregulation
T7630 16291-16293 IN denotes of
T7631 16294-16299 JJ denotes TNF-α
T7632 16300-16310 NN denotes production
T7633 16311-16313 IN denotes in
T7634 16314-16328 JJ denotes LPS-stimulated
T7635 16329-16340 NN denotes mononuclear
T7636 16341-16346 NNS denotes cells
T7637 16347-16350 VBD denotes was
T7638 16351-16359 VBN denotes assessed
T7639 16360-16362 IN denotes by
T7640 16363-16371 VBG denotes treating
T7641 16372-16383 NN denotes mononuclear
T7642 16384-16389 NNS denotes cells
T7643 16390-16394 IN denotes with
T7644 16395-16398 NNP denotes H89
T7645 16398-16399 , denotes ,
T7646 16400-16401 DT denotes a
T7647 16402-16405 NNP denotes PKA
T7648 16406-16415 NN denotes inhibitor
T7649 16415-16416 , denotes ,
T7650 16417-16422 RB denotes prior
T7651 16423-16425 TO denotes to
T7652 16426-16429 NNP denotes LPS
T7653 16430-16433 CC denotes and
T7654 16434-16437 NNP denotes PTX
T7655 16438-16446 NN denotes exposure
T7656 16446-16447 . denotes .
T7657 16448-16451 DT denotes The
T7658 16452-16460 NN denotes addition
T7659 16461-16464 NNP denotes PTX
T7660 16465-16467 TO denotes to
T7661 16468-16482 JJ denotes LPS-stimulated
T7662 16483-16488 NNS denotes cells
T7663 16489-16497 VBD denotes resulted
T7664 16498-16500 IN denotes in
T7665 16501-16502 DT denotes a
T7666 16503-16512 NN denotes reduction
T7667 16513-16515 IN denotes in
T7668 16516-16521 NNP denotes TNF-α
T7669 16521-16522 , denotes ,
T7670 16523-16530 JJ denotes similar
T7671 16531-16533 TO denotes to
T7672 16534-16538 DT denotes that
T7673 16539-16547 VBN denotes observed
T7674 16548-16550 IN denotes in
T7675 16551-16554 PRP$ denotes our
T7676 16555-16563 JJ denotes previous
T7677 16564-16575 NNS denotes experiments
T7678 16576-16577 -LRB- denotes (
T7679 16577-16580 CD denotes 529
T7680 16581-16586 JJ denotes pg/mL
T7681 16587-16588 NN denotes ±
T7682 16589-16592 CD denotes 112
T7683 16593-16596 FW denotes vs.
T7684 16597-16599 CD denotes 37
T7685 16600-16605 NNP denotes pg/mL
T7686 16606-16607 CD denotes ±
T7687 16608-16610 CD denotes 10
T7688 16610-16611 : denotes ;
T7689 16612-16613 NNP denotes P
T7690 16614-16615 NNP denotes <
T7691 16616-16620 CD denotes 0.01
T7692 16620-16621 -RRB- denotes )
T7693 16621-16622 . denotes .
T7694 16623-16637 NN denotes Pre-incubation
T7695 16638-16640 IN denotes of
T7696 16641-16646 NNS denotes cells
T7697 16647-16651 IN denotes with
T7698 16652-16655 NNP denotes H89
T7699 16656-16661 RB denotes prior
T7700 16662-16664 TO denotes to
T7701 16665-16668 NNP denotes LPS
T7702 16669-16672 CC denotes and
T7703 16673-16676 NNP denotes PTX
T7704 16677-16685 NN denotes exposure
T7705 16686-16689 VBD denotes did
T7706 16690-16693 RB denotes not
T7707 16694-16700 VB denotes result
T7708 16701-16703 IN denotes in
T7709 16704-16707 DT denotes any
T7710 16708-16719 JJ denotes significant
T7711 16720-16730 NN denotes modulation
T7712 16731-16733 IN denotes of
T7713 16734-16739 JJ denotes TNF-α
T7714 16740-16750 NN denotes production
T7715 16751-16755 WRB denotes when
T7716 16756-16764 VBN denotes compared
T7717 16765-16767 TO denotes to
T7718 16768-16772 DT denotes that
T7719 16773-16785 VBD denotes demonstrated
T7720 16786-16790 IN denotes with
T7721 16791-16794 NNP denotes PTX
T7722 16795-16800 RB denotes alone
T7723 16801-16802 -LRB- denotes (
T7724 16802-16803 NNP denotes P
T7725 16804-16805 SYM denotes =
T7726 16806-16809 CD denotes 0.2
T7727 16809-16810 : denotes ;
T7728 16811-16817 NN denotes Figure
T7729 16818-16819 CD denotes 5
T7730 16819-16820 -RRB- denotes )
T7731 16820-16821 . denotes .
T7732 16822-16827 DT denotes These
T7733 16828-16835 NNS denotes results
T7734 16836-16843 VBP denotes suggest
T7735 16844-16848 IN denotes that
T7736 16849-16863 NN denotes downregulation
T7737 16864-16866 IN denotes of
T7738 16867-16872 JJ denotes TNF-α
T7739 16873-16883 NN denotes production
T7740 16884-16886 IN denotes by
T7741 16887-16890 NNP denotes PTX
T7742 16891-16893 IN denotes in
T7743 16894-16908 JJ denotes LPS-stimulated
T7744 16909-16920 NN denotes mononuclear
T7745 16921-16926 NNS denotes cells
T7746 16927-16929 VBZ denotes is
T7747 16929-16930 , denotes ,
T7748 16931-16933 IN denotes at
T7749 16934-16939 JJS denotes least
T7750 16940-16942 IN denotes in
T7751 16943-16947 NN denotes part
T7752 16947-16948 , denotes ,
T7753 16949-16964 JJ denotes PKA-independent
T7754 16964-16965 . denotes .
T8411 16978-16981 DT denotes The
T8412 16982-16990 NN denotes exposure
T8413 16991-16993 IN denotes of
T8414 16994-17006 JJ denotes inflammatory
T8415 17007-17012 NNS denotes cells
T8416 17013-17015 TO denotes to
T8417 17016-17017 DT denotes a
T8418 17018-17025 NN denotes variety
T8419 17026-17028 IN denotes of
T8420 17029-17042 JJ denotes extracellular
T8421 17043-17050 NNS denotes stimuli
T8422 17051-17058 NNS denotes results
T8423 17059-17061 IN denotes in
T8424 17062-17065 DT denotes the
T8425 17066-17076 NN denotes initiation
T8426 17077-17079 IN denotes of
T8427 17080-17095 JJ denotes transcriptional
T8428 17096-17099 CC denotes and
T8429 17100-17119 JJ denotes posttranscriptional
T8430 17120-17126 NNS denotes events
T8431 17126-17127 , denotes ,
T8432 17128-17133 WDT denotes which
T8433 17134-17143 VBP denotes culminate
T8434 17144-17146 IN denotes in
T8435 17147-17150 DT denotes the
T8436 17151-17161 NN denotes production
T8437 17162-17164 IN denotes of
T8438 17165-17181 JJ denotes pro-inflammatory
T8439 17182-17191 NNS denotes mediators
T8440 17191-17194 CD denotes .11
T8441 17195-17197 CD denotes 14
T8442 17198-17203 NN denotes TNF-α
T8443 17204-17206 VBZ denotes is
T8444 17207-17215 VBN denotes released
T8445 17216-17220 RB denotes very
T8446 17221-17226 RB denotes early
T8447 17227-17236 VBG denotes following
T8448 17237-17242 NN denotes shock
T8449 17243-17246 CC denotes and
T8450 17247-17249 VBZ denotes is
T8451 17250-17260 VBN denotes considered
T8452 17261-17263 DT denotes an
T8453 17264-17273 JJ denotes important
T8454 17274-17282 NN denotes mediator
T8455 17283-17285 IN denotes of
T8456 17286-17289 DT denotes the
T8457 17290-17302 JJ denotes inflammatory
T8458 17303-17310 NN denotes cascade
T8459 17310-17311 , denotes ,
T8460 17312-17317 IN denotes since
T8461 17318-17320 PRP denotes it
T8462 17321-17330 VBZ denotes regulates
T8463 17331-17334 DT denotes the
T8464 17335-17344 NN denotes synthesis
T8465 17345-17347 IN denotes of
T8466 17348-17355 JJ denotes several
T8467 17356-17361 JJ denotes other
T8468 17362-17370 JJ denotes critical
T8469 17371-17380 NNS denotes cytokines
T8470 17381-17384 CC denotes and
T8471 17385-17395 NNS denotes chemokines
T8472 17395-17398 CD denotes .15
T8473 17399-17400 DT denotes A
T8474 17401-17406 JJ denotes clear
T8475 17407-17419 NN denotes relationship
T8476 17420-17427 IN denotes between
T8477 17428-17437 VBN denotes increased
T8478 17438-17443 NNP denotes TNF-α
T8479 17444-17453 NN denotes synthesis
T8480 17454-17457 CC denotes and
T8481 17458-17461 DT denotes the
T8482 17462-17473 NN denotes development
T8483 17474-17476 IN denotes of
T8484 17477-17482 NN denotes shock
T8485 17483-17486 CC denotes and
T8486 17487-17495 JJ denotes multiple
T8487 17496-17501 NN denotes organ
T8488 17502-17513 NN denotes dysfunction
T8489 17514-17517 VBZ denotes has
T8490 17518-17522 VBN denotes been
T8491 17523-17531 VBN denotes observed
T8492 17532-17534 IN denotes in
T8493 17535-17541 NN denotes animal
T8494 17542-17548 NNS denotes models
T8495 17549-17551 IN denotes of
T8496 17552-17558 NN denotes sepsis
T8497 17558-17561 CD denotes .15
T8498 17562-17564 CD denotes 17
T8499 17565-17572 IN denotes Because
T8500 17573-17575 IN denotes of
T8501 17576-17579 PRP$ denotes its
T8502 17580-17590 NN denotes importance
T8503 17591-17593 IN denotes in
T8504 17594-17607 VBG denotes orchestrating
T8505 17608-17616 JJ denotes multiple
T8506 17617-17622 NNS denotes steps
T8507 17623-17625 IN denotes of
T8508 17626-17629 DT denotes the
T8509 17630-17642 JJ denotes inflammatory
T8510 17643-17651 NN denotes response
T8511 17651-17652 , denotes ,
T8512 17653-17663 NNS denotes strategies
T8513 17664-17669 VBN denotes aimed
T8514 17670-17672 IN denotes at
T8515 17673-17683 VBG denotes decreasing
T8516 17684-17689 JJ denotes TNF-α
T8517 17690-17696 NNS denotes levels
T8518 17697-17699 CC denotes or
T8519 17700-17711 VBG denotes attenuating
T8520 17712-17715 PRP$ denotes its
T8521 17716-17726 NN denotes production
T8522 17727-17731 VBP denotes seem
T8523 17732-17742 JJ denotes attractive
T8524 17742-17743 . denotes .
T8525 17744-17747 NNP denotes PDE
T8526 17748-17758 NNS denotes inhibitors
T8527 17759-17763 VBP denotes have
T8528 17764-17768 VBN denotes been
T8529 17769-17774 VBN denotes shown
T8530 17775-17777 TO denotes to
T8531 17778-17790 VB denotes downregulate
T8532 17791-17796 JJ denotes TNF-α
T8533 17797-17807 NN denotes production
T8534 17808-17810 IN denotes in
T8535 17811-17819 JJ denotes distinct
T8536 17820-17825 JJ denotes human
T8537 17826-17830 NN denotes cell
T8538 17831-17842 NNS denotes populations
T8539 17842-17843 . denotes .
T8540 17844-17846 PRP denotes We
T8541 17847-17851 VBP denotes have
T8542 17852-17862 RB denotes previously
T8543 17863-17868 VBN denotes shown
T8544 17869-17873 IN denotes that
T8545 17873-17874 , denotes ,
T8546 17875-17877 IN denotes in
T8547 17878-17885 JJ denotes general
T8548 17885-17886 , denotes ,
T8549 17887-17892 JJ denotes TNF-α
T8550 17893-17899 NNS denotes levels
T8551 17900-17908 VBP denotes continue
T8552 17909-17911 TO denotes to
T8553 17912-17920 VB denotes increase
T8554 17921-17926 IN denotes after
T8555 17927-17930 NNP denotes LPS
T8556 17931-17939 NN denotes exposure
T8557 17940-17943 CC denotes and
T8558 17944-17948 IN denotes that
T8559 17949-17960 JJ denotes concomitant
T8560 17961-17969 NN denotes infusion
T8561 17970-17972 IN denotes of
T8562 17973-17990 NN denotes phosphodiesterase
T8563 17991-18001 NNS denotes inhibitors
T8564 18002-18005 MD denotes can
T8565 18006-18014 RB denotes markedly
T8566 18015-18023 VB denotes decrease
T8567 18024-18029 JJ denotes TNF-α
T8568 18030-18036 NNS denotes levels
T8569 18036-18039 CD denotes .18
T8570 18040-18042 CD denotes 19
T8571 18043-18045 IN denotes In
T8572 18046-18050 DT denotes this
T8573 18051-18053 IN denotes in
T8574 18054-18059 NN denotes vitro
T8575 18060-18065 NN denotes study
T8576 18065-18066 , denotes ,
T8577 18067-18069 PRP denotes we
T8578 18070-18078 VBD denotes verified
T8579 18079-18082 DT denotes the
T8580 18083-18092 VBN denotes sustained
T8581 18093-18104 NN denotes attenuation
T8582 18105-18107 IN denotes of
T8583 18108-18119 JJ denotes LPS-induced
T8584 18120-18125 JJ denotes TNF-α
T8585 18126-18136 NN denotes expression
T8586 18137-18141 IN denotes over
T8587 18142-18146 NN denotes time
T8588 18147-18149 IN denotes in
T8589 18150-18152 DT denotes an
T8590 18153-18161 JJ denotes isolated
T8591 18162-18167 JJ denotes human
T8592 18168-18179 NN denotes mononuclear
T8593 18180-18184 NN denotes cell
T8594 18185-18195 NN denotes population
T8595 18195-18196 . denotes .
T8596 18197-18200 DT denotes The
T8597 18201-18211 NNS denotes mechanisms
T8598 18212-18214 IN denotes by
T8599 18215-18220 WDT denotes which
T8600 18221-18224 NNP denotes PTX
T8601 18224-18225 , denotes ,
T8602 18226-18227 DT denotes a
T8603 18228-18240 JJ denotes non-specific
T8604 18241-18258 NN denotes phosphodiesterase
T8605 18259-18268 NN denotes inhibitor
T8606 18268-18269 , denotes ,
T8607 18270-18283 RB denotes significantly
T8608 18284-18287 CC denotes and
T8609 18288-18300 RB denotes consistently
T8610 18301-18310 VBZ denotes decreases
T8611 18311-18316 JJ denotes TNF-α
T8612 18317-18327 NN denotes production
T8613 18328-18332 VBP denotes have
T8614 18333-18336 RB denotes not
T8615 18337-18341 VBN denotes been
T8616 18342-18352 RB denotes completely
T8617 18353-18363 VBN denotes elucidated
T8618 18363-18364 . denotes .
T8619 18365-18367 PRP denotes It
T8620 18368-18371 VBZ denotes has
T8621 18372-18376 VBN denotes been
T8622 18377-18387 VBN denotes postulated
T8623 18388-18392 IN denotes that
T8624 18393-18398 NNS denotes drugs
T8625 18399-18403 WDT denotes that
T8626 18404-18412 VBP denotes increase
T8627 18413-18426 JJ denotes intracellular
T8628 18427-18431 NNP denotes cAMP
T8629 18432-18437 VB denotes exert
T8630 18438-18443 PRP$ denotes their
T8631 18444-18461 JJ denotes anti-inflammatory
T8632 18462-18469 NNS denotes effects
T8633 18470-18477 IN denotes through
T8634 18478-18481 DT denotes the
T8635 18482-18492 NN denotes activation
T8636 18493-18495 IN denotes of
T8637 18496-18499 NNP denotes PKA
T8638 18499-18500 . denotes .
T8639 18501-18505 DT denotes This
T8640 18506-18513 JJ denotes classic
T8641 18514-18521 NN denotes pathway
T8642 18522-18525 VBZ denotes has
T8643 18526-18530 VBN denotes been
T8644 18531-18541 VBN denotes challenged
T8645 18542-18544 IN denotes as
T8646 18545-18548 DT denotes the
T8647 18549-18553 JJ denotes sole
T8648 18554-18563 NN denotes mechanism
T8649 18564-18572 VBN denotes involved
T8650 18573-18575 IN denotes in
T8651 18576-18579 DT denotes the
T8652 18580-18590 NN denotes modulation
T8653 18591-18593 IN denotes of
T8654 18594-18606 NN denotes inflammation
T8655 18607-18609 IN denotes by
T8656 18610-18624 JJ denotes cAMP-enhancing
T8657 18625-18630 NNS denotes drugs
T8658 18630-18631 , denotes ,
T8659 18632-18635 CC denotes and
T8660 18636-18647 JJ denotes alternative
T8661 18648-18658 NNS denotes mechanisms
T8662 18659-18668 VBG denotes involving
T8663 18669-18684 JJ denotes PKA-independent
T8664 18685-18693 NNS denotes pathways
T8665 18694-18698 VBP denotes have
T8666 18699-18703 VBN denotes been
T8667 18704-18712 VBN denotes proposed
T8668 18712-18714 CD denotes .6
T8669 18715-18716 CD denotes 8
T8670 18717-18719 IN denotes In
T8671 18720-18724 DT denotes this
T8672 18725-18731 NN denotes series
T8673 18732-18734 IN denotes of
T8674 18735-18746 NNS denotes experiments
T8675 18746-18747 , denotes ,
T8676 18748-18750 PRP denotes we
T8677 18751-18759 VBD denotes observed
T8678 18760-18761 DT denotes a
T8679 18762-18773 JJ denotes significant
T8680 18774-18785 NN denotes attenuation
T8681 18786-18788 IN denotes of
T8682 18789-18794 JJ denotes TNF-α
T8683 18795-18805 NN denotes production
T8684 18806-18808 IN denotes in
T8685 18809-18823 JJ denotes LPS-stimulated
T8686 18824-18829 JJ denotes human
T8687 18830-18841 NN denotes mononuclear
T8688 18842-18847 NNS denotes cells
T8689 18848-18857 VBG denotes following
T8690 18858-18861 NNP denotes PTX
T8691 18862-18876 NN denotes administration
T8692 18876-18877 , denotes ,
T8693 18878-18889 JJ denotes independent
T8694 18890-18892 IN denotes of
T8695 18893-18896 NNP denotes PKA
T8696 18897-18907 NN denotes activation
T8697 18907-18908 . denotes .
T8698 18909-18911 PRP denotes We
T8699 18912-18916 VBD denotes used
T8700 18917-18918 DT denotes a
T8701 18919-18930 JJ denotes nonspecific
T8702 18931-18948 NN denotes phosphodiesterase
T8703 18949-18958 NN denotes inhibitor
T8704 18958-18959 , denotes ,
T8705 18960-18963 NNP denotes PTX
T8706 18963-18964 , denotes ,
T8707 18965-18967 IN denotes in
T8708 18968-18971 DT denotes the
T8709 18972-18979 JJ denotes present
T8710 18980-18985 NN denotes study
T8711 18986-18993 IN denotes because
T8712 18994-18996 PRP denotes it
T8713 18997-19000 VBZ denotes has
T8714 19001-19005 VBN denotes been
T8715 19006-19010 VBN denotes used
T8716 19011-19021 RB denotes clinically
T8717 19022-19024 IN denotes in
T8718 19025-19028 DT denotes the
T8719 19029-19038 NN denotes treatment
T8720 19039-19041 IN denotes of
T8721 19042-19043 DT denotes a
T8722 19044-19051 NN denotes variety
T8723 19052-19054 IN denotes of
T8724 19055-19065 NNS denotes conditions
T8725 19066-19068 IN denotes in
T8726 19069-19074 WDT denotes which
T8727 19075-19087 NN denotes inflammation
T8728 19088-19090 VBZ denotes is
T8729 19091-19093 DT denotes an
T8730 19094-19103 JJ denotes important
T8731 19104-19113 NN denotes component
T8732 19114-19116 IN denotes of
T8733 19117-19120 DT denotes the
T8734 19121-19136 NN denotes pathophysiology
T8735 19137-19139 IN denotes of
T8736 19140-19143 DT denotes the
T8737 19144-19151 NN denotes disease
T8738 19152-19159 NN denotes process
T8739 19159-19162 CD denotes .19
T8740 19163-19175 RB denotes Additionally
T8741 19175-19176 , denotes ,
T8742 19177-19180 DT denotes the
T8743 19181-19184 NN denotes use
T8744 19185-19187 IN denotes of
T8745 19188-19191 NNP denotes PTX
T8746 19192-19194 IN denotes in
T8747 19195-19201 NN denotes sepsis
T8748 19202-19204 IN denotes as
T8749 19205-19207 DT denotes an
T8750 19208-19215 NN denotes adjunct
T8751 19216-19218 TO denotes to
T8752 19219-19224 JJ denotes other
T8753 19225-19235 NNS denotes treatments
T8754 19236-19238 TO denotes to
T8755 19239-19247 VB denotes maintain
T8756 19248-19256 JJ denotes adequate
T8757 19257-19262 NN denotes organ
T8758 19263-19271 NN denotes function
T8759 19272-19275 VBZ denotes has
T8760 19276-19280 VBN denotes been
T8761 19281-19289 VBN denotes explored
T8762 19290-19295 VBN denotes based
T8763 19296-19300 IN denotes upon
T8764 19301-19304 PRP$ denotes its
T8765 19305-19312 NNS denotes effects
T8766 19313-19315 IN denotes on
T8767 19316-19321 JJ denotes TNF-α
T8768 19322-19331 NN denotes synthesis
T8769 19331-19332 . denotes .
T8770 19333-19340 RB denotes However
T8771 19340-19341 , denotes ,
T8772 19342-19345 DT denotes the
T8773 19346-19354 VBN denotes reported
T8774 19355-19365 JJ denotes beneficial
T8775 19366-19373 NNS denotes effects
T8776 19374-19376 IN denotes of
T8777 19377-19380 NNP denotes PTX
T8778 19381-19384 VBP denotes are
T8779 19385-19388 RB denotes not
T8780 19389-19393 RB denotes only
T8781 19394-19401 VBN denotes related
T8782 19402-19404 TO denotes to
T8783 19405-19408 DT denotes the
T8784 19409-19423 NN denotes downregulation
T8785 19424-19426 IN denotes of
T8786 19427-19435 NNP denotes TNF-αbut
T8787 19436-19440 RB denotes also
T8788 19441-19443 TO denotes to
T8789 19444-19447 NNP denotes PTX
T8790 19450-19464 JJ denotes hemorrheologic
T8791 19465-19475 NNS denotes properties
T8792 19475-19476 , denotes ,
T8793 19477-19480 PRP$ denotes its
T8794 19481-19488 NN denotes ability
T8795 19489-19491 TO denotes to
T8796 19492-19498 VB denotes reduce
T8797 19499-19509 NN denotes neutrophil
T8798 19510-19520 NN denotes activation
T8799 19520-19521 , denotes ,
T8800 19522-19525 CC denotes and
T8801 19526-19529 PRP$ denotes its
T8802 19530-19540 JJ denotes beneficial
T8803 19541-19548 NNS denotes effects
T8804 19549-19551 IN denotes on
T8805 19552-19568 NN denotes microcirculation
T8806 19568-19569 , denotes ,
T8807 19570-19577 JJ denotes cardiac
T8808 19578-19589 NN denotes performance
T8809 19589-19590 , denotes ,
T8810 19591-19594 CC denotes and
T8811 19595-19600 NN denotes organ
T8812 19601-19607 NN denotes injury
T8813 19607-19610 CD denotes .20
T8814 19611-19613 CD denotes 25
T8815 19614-19620 NNP denotes Herein
T8816 19620-19621 , denotes ,
T8817 19622-19624 PRP denotes we
T8818 19625-19637 VBD denotes demonstrated
T8819 19638-19642 IN denotes that
T8820 19643-19646 DT denotes the
T8821 19647-19661 JJ denotes downregulatory
T8822 19662-19669 NNS denotes effects
T8823 19670-19672 IN denotes of
T8824 19673-19676 NNP denotes PTX
T8825 19677-19679 IN denotes in
T8826 19680-19685 JJ denotes human
T8827 19686-19697 NN denotes mononuclear
T8828 19698-19703 NNS denotes cells
T8829 19704-19712 VBZ denotes involves
T8830 19713-19723 NN denotes modulation
T8831 19724-19726 IN denotes of
T8832 19727-19735 NNS denotes pathways
T8833 19736-19740 WDT denotes that
T8834 19741-19748 VBP denotes involve
T8835 19749-19754 NNP denotes NF-κB
T8836 19755-19758 CC denotes and
T8837 19759-19763 NNP denotes CREB
T8838 19763-19764 , denotes ,
T8839 19765-19768 CD denotes two
T8840 19769-19774 JJ denotes major
T8841 19775-19788 NN denotes transcription
T8842 19789-19796 NNS denotes factors
T8843 19797-19805 VBN denotes involved
T8844 19806-19808 IN denotes in
T8845 19809-19812 DT denotes the
T8846 19813-19825 JJ denotes inflammatory
T8847 19826-19833 NN denotes cascade
T8848 19833-19834 . denotes .
T8849 19835-19840 NNP denotes NF-κB
T8850 19840-19841 NNP denotes /
T8851 19841-19844 NNP denotes Rel
T8852 19845-19856 VBZ denotes constitutes
T8853 19857-19858 DT denotes a
T8854 19859-19865 NN denotes family
T8855 19866-19868 IN denotes of
T8856 19869-19884 JJ denotes transcriptional
T8857 19885-19892 NNS denotes factors
T8858 19893-19901 VBN denotes involved
T8859 19902-19904 IN denotes in
T8860 19905-19908 DT denotes the
T8861 19909-19919 NN denotes regulation
T8862 19920-19922 IN denotes of
T8863 19923-19931 JJ denotes numerous
T8864 19932-19940 NN denotes cytokine
T8865 19941-19946 NNS denotes genes
T8866 19947-19950 CC denotes and
T8867 19951-19957 JJ denotes immune
T8868 19958-19967 NNS denotes responses
T8869 19968-19970 IN denotes in
T8870 19971-19980 JJ denotes different
T8871 19981-19985 NN denotes cell
T8872 19986-19997 NNS denotes populations
T8873 19997-19998 . denotes .
T8874 19999-20002 DT denotes The
T8875 20003-20007 RBS denotes most
T8876 20008-20016 JJ denotes abundant
T8877 20017-20021 NN denotes form
T8878 20022-20024 IN denotes of
T8879 20025-20030 NNP denotes NF-κB
T8880 20031-20033 VBZ denotes is
T8881 20034-20037 DT denotes the
T8882 20038-20041 CD denotes p50
T8883 20042-20045 CD denotes p65
T8884 20046-20057 NN denotes heterodimer
T8885 20057-20060 CD denotes .26
T8886 20061-20064 DT denotes The
T8887 20065-20073 JJ denotes inactive
T8888 20074-20078 NN denotes form
T8889 20079-20081 IN denotes of
T8890 20082-20087 JJ denotes NF-κB
T8891 20088-20094 VBZ denotes exists
T8892 20095-20097 IN denotes in
T8893 20098-20101 DT denotes the
T8894 20102-20111 NN denotes cytoplasm
T8895 20112-20117 VBN denotes bound
T8896 20118-20120 TO denotes to
T8897 20121-20123 DT denotes an
T8898 20124-20134 JJ denotes inhibitory
T8899 20135-20142 JJ denotes complex
T8900 20143-20153 VBG denotes containing
T8901 20154-20159 NNP denotes I-κBα
T8902 20159-20160 . denotes .
T8903 20161-20170 VBG denotes Following
T8904 20171-20179 JJ denotes cellular
T8905 20180-20191 NN denotes stimulation
T8906 20192-20196 IN denotes with
T8907 20197-20200 NNP denotes LPS
T8908 20201-20204 CC denotes and
T8909 20205-20216 JJ denotes chemotactic
T8910 20217-20224 NNS denotes factors
T8911 20224-20225 , denotes ,
T8912 20226-20231 JJ denotes NF-κB
T8913 20232-20241 JJ denotes inducible
T8914 20242-20248 NN denotes kinase
T8915 20249-20263 NNS denotes phosphorylates
T8916 20264-20267 CC denotes and
T8917 20268-20277 VBZ denotes activates
T8918 20278-20281 DT denotes the
T8919 20282-20286 JJ denotes I-κB
T8920 20287-20293 NN denotes kinase
T8921 20294-20295 -LRB- denotes (
T8922 20295-20298 NNP denotes IKK
T8923 20298-20299 -RRB- denotes )
T8924 20300-20307 JJ denotes complex
T8925 20308-20318 VBG denotes consisting
T8926 20319-20321 IN denotes of
T8927 20322-20327 NN denotes IKK-1
T8928 20328-20331 CC denotes and
T8929 20332-20337 NN denotes IKK-2
T8930 20337-20338 , denotes ,
T8931 20339-20344 WDT denotes which
T8932 20345-20347 IN denotes in
T8933 20348-20352 NN denotes turn
T8934 20353-20367 VBZ denotes phosphorylates
T8935 20368-20371 DT denotes the
T8936 20372-20377 JJ denotes I-κBα
T8937 20378-20385 NN denotes subunit
T8938 20385-20386 . denotes .
T8939 20387-20402 NNP denotes Phosphorylation
T8940 20402-20403 , denotes ,
T8941 20404-20418 NN denotes ubiquitination
T8942 20418-20419 , denotes ,
T8943 20420-20423 CC denotes and
T8944 20424-20434 JJ denotes subsequent
T8945 20435-20446 NN denotes degradation
T8946 20447-20449 IN denotes of
T8947 20450-20455 JJ denotes I-κBα
T8948 20456-20463 NNS denotes results
T8949 20464-20466 IN denotes in
T8950 20467-20472 JJ denotes NF-κB
T8951 20473-20480 JJ denotes nuclear
T8952 20481-20494 NN denotes translocation
T8953 20494-20495 . denotes .
T8954 20496-20500 DT denotes This
T8955 20501-20507 VBZ denotes allows
T8956 20508-20511 DT denotes the
T8957 20512-20525 NN denotes transcription
T8958 20526-20532 NN denotes factor
T8959 20533-20535 TO denotes to
T8960 20536-20540 NN denotes bind
T8961 20541-20543 TO denotes to
T8962 20544-20552 NN denotes promoter
T8963 20553-20560 NNS denotes regions
T8964 20561-20563 IN denotes of
T8965 20564-20572 JJ denotes specific
T8966 20573-20589 JJ denotes pro-inflammatory
T8967 20590-20595 NNS denotes genes
T8968 20596-20599 CC denotes and
T8969 20600-20609 NN denotes influence
T8970 20610-20623 NN denotes transcription
T8971 20623-20629 CD denotes .27,28
T8972 20630-20637 IN denotes Because
T8973 20638-20639 DT denotes a
T8974 20640-20646 NN denotes number
T8975 20647-20649 IN denotes of
T8976 20650-20659 JJ denotes potential
T8977 20660-20662 NN denotes κB
T8978 20663-20668 NNS denotes sites
T8979 20669-20672 VBP denotes are
T8980 20673-20680 JJ denotes present
T8981 20681-20683 IN denotes in
T8982 20684-20687 DT denotes the
T8983 20688-20698 NN denotes nucleotide
T8984 20699-20708 NNS denotes sequences
T8985 20709-20711 IN denotes of
T8986 20712-20715 DT denotes the
T8987 20716-20721 NNP denotes TNF-α
T8988 20722-20726 NN denotes gene
T8989 20726-20727 , denotes ,
T8990 20728-20733 NNP denotes NF-κB
T8991 20734-20736 VBZ denotes is
T8992 20737-20747 VBN denotes considered
T8993 20748-20750 TO denotes to
T8994 20751-20753 VB denotes be
T8995 20754-20755 DT denotes a
T8996 20756-20764 JJ denotes critical
T8997 20765-20780 JJ denotes transcriptional
T8998 20781-20787 NN denotes factor
T8999 20788-20796 VBN denotes involved
T9000 20797-20799 IN denotes in
T9001 20800-20805 NNP denotes TNF-α
T9002 20806-20810 NN denotes gene
T9003 20811-20821 NN denotes expression
T9004 20822-20825 CC denotes and
T9005 20826-20833 NN denotes protein
T9006 20834-20843 NN denotes synthesis
T9007 20843-20849 CD denotes .29,30
T9008 20850-20852 IN denotes In
T9009 20853-20857 DT denotes this
T9010 20858-20861 NN denotes set
T9011 20862-20864 IN denotes of
T9012 20865-20876 NNS denotes experiments
T9013 20876-20877 , denotes ,
T9014 20878-20880 PRP denotes we
T9015 20881-20893 VBD denotes demonstrated
T9016 20894-20898 IN denotes that
T9017 20899-20902 NNP denotes PTX
T9018 20903-20916 VBZ denotes downregulates
T9019 20917-20928 JJ denotes cytoplasmic
T9020 20929-20934 JJ denotes I-κBα
T9021 20935-20950 NN denotes phosphorylation
T9022 20950-20951 , denotes ,
T9023 20952-20959 JJ denotes nuclear
T9024 20960-20965 NNP denotes NF-κB
T9025 20966-20969 CD denotes p65
T9026 20970-20999 NN denotes phosphorylation/translocation
T9027 20999-21000 , denotes ,
T9028 21001-21003 RB denotes as
T9029 21004-21008 RB denotes well
T9030 21009-21011 IN denotes as
T9031 21012-21017 NNP denotes NF-κB
T9032 21018-21029 NNP denotes DNA-binding
T9033 21030-21035 IN denotes after
T9034 21036-21039 NNP denotes LPS
T9035 21040-21051 NN denotes stimulation
T9036 21051-21052 , denotes ,
T9037 21053-21063 VBG denotes suggesting
T9038 21064-21068 IN denotes that
T9039 21069-21072 NNP denotes PTX
T9040 21073-21079 VBZ denotes exerts
T9041 21080-21083 PRP$ denotes its
T9042 21084-21092 NN denotes function
T9043 21093-21101 NN denotes proximal
T9044 21102-21104 TO denotes to
T9045 21105-21107 CC denotes or
T9046 21108-21110 IN denotes at
T9047 21111-21114 DT denotes the
T9048 21115-21120 NN denotes level
T9049 21121-21123 IN denotes of
T9050 21124-21129 JJ denotes I-κBα
T9051 21130-21145 NN denotes phosphorylation
T9052 21145-21146 . denotes .
T9053 21147-21155 JJ denotes Previous
T9054 21156-21163 NNS denotes studies
T9055 21164-21168 WDT denotes that
T9056 21169-21173 VBP denotes have
T9057 21174-21183 VBN denotes evaluated
T9058 21184-21187 DT denotes the
T9059 21188-21195 NNS denotes effects
T9060 21196-21198 IN denotes of
T9061 21199-21213 JJ denotes cAMP-elevating
T9062 21214-21219 NNS denotes drugs
T9063 21220-21222 IN denotes on
T9064 21223-21228 NNP denotes I-κBα
T9065 21229-21232 CC denotes and
T9066 21233-21238 NNP denotes NF-κB
T9067 21239-21243 VBP denotes have
T9068 21244-21252 VBN denotes reported
T9069 21253-21264 VBG denotes conflicting
T9070 21265-21272 NNS denotes results
T9071 21272-21273 . denotes .
T9072 21274-21280 NNP denotes Haddad
T9073 21281-21283 FW denotes et
T9074 21284-21287 FW denotes al.
T9075 21288-21296 VBD denotes reported
T9076 21297-21304 NNS denotes results
T9077 21305-21312 JJ denotes similar
T9078 21313-21315 TO denotes to
T9079 21316-21320 JJ denotes ours
T9080 21321-21325 IN denotes with
T9081 21326-21333 NN denotes respect
T9082 21334-21336 TO denotes to
T9083 21337-21340 DT denotes the
T9084 21341-21348 NNS denotes effects
T9085 21349-21351 IN denotes of
T9086 21352-21355 NNP denotes PTX
T9087 21356-21358 IN denotes on
T9088 21359-21364 NNP denotes I-κBα
T9089 21365-21368 CC denotes and
T9090 21369-21374 NNP denotes NF-κB
T9091 21375-21377 IN denotes in
T9092 21378-21387 JJ denotes pulmonary
T9093 21388-21398 JJ denotes epithelial
T9094 21399-21404 NNS denotes cells
T9095 21404-21407 CD denotes .31
T9096 21408-21418 RB denotes Conversely
T9097 21418-21419 , denotes ,
T9098 21420-21427 NNP denotes Neumann
T9099 21428-21430 FW denotes et
T9100 21431-21434 FW denotes al.
T9101 21435-21443 VBD denotes proposed
T9102 21444-21448 IN denotes that
T9103 21449-21458 VBN denotes increased
T9104 21459-21472 JJ denotes intracellular
T9105 21473-21477 NNP denotes cAMP
T9106 21478-21488 VBZ denotes stabilizes
T9107 21489-21492 DT denotes the
T9108 21493-21504 NN denotes interaction
T9109 21505-21512 IN denotes between
T9110 21513-21518 NNP denotes I-κBα
T9111 21519-21522 CC denotes and
T9112 21523-21528 NNP denotes NF-κB
T9113 21528-21529 , denotes ,
T9114 21530-21539 RB denotes therefore
T9115 21540-21548 VBG denotes reducing
T9116 21549-21554 JJ denotes NF-κB
T9117 21555-21565 NN denotes activation
T9118 21565-21568 CD denotes .32
T9119 21569-21571 IN denotes In
T9120 21572-21580 NN denotes contrast
T9121 21580-21581 , denotes ,
T9122 21582-21591 NNP denotes Takahashi
T9123 21592-21594 FW denotes et
T9124 21595-21598 FW denotes al.
T9125 21599-21605 VBD denotes showed
T9126 21606-21608 DT denotes no
T9127 21609-21619 NN denotes difference
T9128 21620-21622 IN denotes in
T9129 21623-21628 JJ denotes I-κBα
T9130 21629-21640 NN denotes degradation
T9131 21641-21643 IN denotes in
T9132 21644-21650 NNP denotes Jurkat
T9133 21651-21664 NNS denotes T-lymphocytes
T9134 21665-21667 IN denotes in
T9135 21668-21671 DT denotes the
T9136 21672-21680 NN denotes presence
T9137 21681-21684 CC denotes and
T9138 21685-21692 NN denotes absence
T9139 21693-21695 IN denotes of
T9140 21696-21705 NN denotes forskolin
T9141 21705-21706 , denotes ,
T9142 21707-21709 DT denotes an
T9143 21710-21721 JJ denotes alternative
T9144 21722-21726 NNP denotes cAMP
T9145 21727-21735 VBG denotes inducing
T9146 21736-21744 NN denotes compound
T9147 21744-21745 . denotes .
T9148 21746-21750 DT denotes This
T9149 21751-21756 NN denotes group
T9150 21757-21761 RB denotes also
T9151 21762-21774 VBD denotes demonstrated
T9152 21775-21779 IN denotes that
T9153 21780-21789 NN denotes forskolin
T9154 21790-21793 VBD denotes did
T9155 21794-21797 RB denotes not
T9156 21798-21805 VB denotes inhibit
T9157 21806-21809 DT denotes the
T9158 21810-21821 JJ denotes DNA-binding
T9159 21822-21830 NN denotes activity
T9160 21831-21833 IN denotes of
T9161 21834-21839 NNP denotes NF-κB
T9162 21840-21843 CC denotes and
T9163 21844-21848 IN denotes that
T9164 21849-21853 NNP denotes CREB
T9165 21854-21857 VBD denotes was
T9166 21858-21861 RB denotes not
T9167 21862-21870 VBN denotes involved
T9168 21871-21873 IN denotes in
T9169 21874-21891 JJ denotes forskolin-induced
T9170 21892-21900 NN denotes decrease
T9171 21901-21903 IN denotes in
T9172 21904-21907 CD denotes p65
T9173 21908-21923 JJ denotes transcriptional
T9174 21924-21932 NN denotes activity
T9175 21932-21935 CD denotes .33
T9176 21936-21943 JJ denotes Similar
T9177 21944-21951 NNS denotes results
T9178 21952-21956 VBD denotes were
T9179 21957-21965 VBN denotes reported
T9180 21966-21968 IN denotes by
T9181 21969-21977 NNP denotes Ollivier
T9182 21978-21980 FW denotes et
T9183 21981-21984 FW denotes al.
T9184 21985-21990 VBG denotes using
T9185 21991-21996 CD denotes THP-1
T9186 21997-22002 NNS denotes cells
T9187 22003-22006 CC denotes and
T9188 22007-22018 JJ denotes endothelial
T9189 22019-22024 NNS denotes cells
T9190 22025-22032 VBN denotes treated
T9191 22033-22037 IN denotes with
T9192 22038-22047 NN denotes forskolin
T9193 22048-22050 CC denotes or
T9194 22051-22060 NN denotes dibutyryl
T9195 22061-22065 NNP denotes cAMP
T9196 22065-22066 , denotes ,
T9197 22067-22070 CD denotes two
T9198 22071-22077 NNS denotes agents
T9199 22078-22083 VBN denotes known
T9200 22084-22086 TO denotes to
T9201 22087-22095 VB denotes function
T9202 22096-22103 IN denotes through
T9203 22104-22108 NNP denotes cAMP
T9204 22109-22118 NN denotes elevation
T9205 22119-22122 CC denotes and
T9206 22123-22133 JJ denotes subsequent
T9207 22134-22137 NNP denotes PKA
T9208 22138-22148 NN denotes activation
T9209 22148-22150 CD denotes .9
T9210 22151-22154 DT denotes The
T9211 22155-22166 NNS denotes differences
T9212 22167-22174 IN denotes between
T9213 22175-22178 PRP$ denotes our
T9214 22179-22186 NNS denotes results
T9215 22187-22190 CC denotes and
T9216 22191-22196 DT denotes those
T9217 22197-22206 VBN denotes mentioned
T9218 22207-22212 IN denotes above
T9219 22213-22216 MD denotes may
T9220 22217-22219 VB denotes be
T9221 22220-22229 VBN denotes explained
T9222 22230-22232 IN denotes by
T9223 22233-22236 DT denotes the
T9224 22237-22240 NN denotes use
T9225 22241-22243 IN denotes of
T9226 22244-22253 JJ denotes different
T9227 22254-22258 NN denotes cell
T9228 22259-22264 NNS denotes types
T9229 22265-22268 CC denotes and
T9230 22269-22278 JJ denotes exogenous
T9231 22279-22289 NNS denotes stimulants
T9232 22289-22290 . denotes .
T9233 22291-22293 PRP denotes It
T9234 22294-22296 VBZ denotes is
T9235 22297-22301 RB denotes also
T9236 22302-22310 JJ denotes possible
T9237 22311-22315 IN denotes that
T9238 22316-22319 NNP denotes PTX
T9239 22320-22326 VBZ denotes exerts
T9240 22327-22330 PRP$ denotes its
T9241 22331-22348 JJ denotes anti-inflammatory
T9242 22349-22356 NNS denotes effects
T9243 22357-22359 IN denotes on
T9244 22360-22363 DT denotes the
T9245 22364-22369 NNP denotes NF-κB
T9246 22370-22377 NN denotes pathway
T9247 22378-22389 JJ denotes independent
T9248 22390-22392 IN denotes of
T9249 22393-22396 NNP denotes PKA
T9250 22396-22397 , denotes ,
T9251 22398-22400 IN denotes as
T9252 22401-22410 VBN denotes suggested
T9253 22411-22413 IN denotes by
T9254 22414-22417 DT denotes the
T9255 22418-22427 VBN denotes continued
T9256 22428-22439 NN denotes attenuation
T9257 22440-22442 IN denotes of
T9258 22443-22454 JJ denotes LPS-induced
T9259 22455-22460 JJ denotes TNF-α
T9260 22461-22471 NN denotes production
T9261 22472-22474 IN denotes by
T9262 22475-22478 NNP denotes PTX
T9263 22479-22481 IN denotes in
T9264 22482-22485 DT denotes the
T9265 22486-22494 NN denotes presence
T9266 22495-22497 CC denotes or
T9267 22498-22505 NN denotes absence
T9268 22506-22508 IN denotes of
T9269 22509-22512 NNP denotes PKA
T9270 22513-22523 NN denotes inhibition
T9271 22524-22532 VBD denotes observed
T9272 22533-22535 IN denotes in
T9273 22536-22540 DT denotes this
T9274 22541-22546 NN denotes study
T9275 22546-22547 . denotes .
T9276 22548-22552 DT denotes This
T9277 22553-22563 NN denotes hypothesis
T9278 22564-22566 VBZ denotes is
T9279 22567-22576 RB denotes currently
T9280 22577-22582 IN denotes under
T9281 22583-22596 NN denotes investigation
T9282 22597-22599 IN denotes in
T9283 22600-22603 PRP$ denotes our
T9284 22604-22614 NN denotes laboratory
T9285 22614-22615 . denotes .
T9286 22616-22627 RB denotes Furthermore
T9287 22627-22628 , denotes ,
T9288 22629-22631 PRP denotes we
T9289 22632-22640 VBD denotes utilized
T9290 22641-22642 DT denotes a
T9291 22643-22648 JJ denotes broad
T9292 22649-22652 NNP denotes PKA
T9293 22653-22662 NN denotes inhibitor
T9294 22663-22665 IN denotes in
T9295 22666-22670 DT denotes this
T9296 22671-22676 NN denotes study
T9297 22677-22679 IN denotes in
T9298 22680-22688 NN denotes addition
T9299 22689-22691 TO denotes to
T9300 22692-22695 NNP denotes H89
T9301 22695-22696 , denotes ,
T9302 22697-22700 CC denotes but
T9303 22701-22704 VBD denotes did
T9304 22705-22708 RB denotes not
T9305 22709-22716 VB denotes examine
T9306 22717-22720 DT denotes the
T9307 22721-22727 NN denotes effect
T9308 22728-22730 IN denotes of
T9309 22731-22739 JJ denotes specific
T9310 22740-22750 NN denotes inhibition
T9311 22751-22753 IN denotes of
T9312 22754-22761 JJ denotes various
T9313 22762-22765 NNP denotes PKA
T9314 22766-22774 NNS denotes isoforms
T9315 22774-22775 . denotes .
T9316 22776-22780 DT denotes This
T9317 22781-22792 NN denotes information
T9318 22793-22796 MD denotes may
T9319 22797-22802 VB denotes yield
T9320 22803-22807 JJR denotes more
T9321 22808-22815 NN denotes insight
T9322 22816-22820 IN denotes into
T9323 22821-22824 DT denotes the
T9324 22825-22834 NN denotes mechanism
T9325 22835-22837 IN denotes by
T9326 22838-22843 WDT denotes which
T9327 22844-22847 NNP denotes PTX
T9328 22848-22854 VBZ denotes exerts
T9329 22855-22858 PRP$ denotes its
T9330 22859-22874 JJ denotes down-regulatory
T9331 22875-22882 NNS denotes effects
T9332 22882-22883 . denotes .
T9333 22884-22887 NNP denotes PDE
T9334 22888-22898 NNS denotes inhibitors
T9335 22899-22902 MD denotes may
T9336 22903-22907 RB denotes also
T9337 22908-22920 VB denotes downregulate
T9338 22921-22926 JJ denotes NF-κB
T9339 22927-22942 JJ denotes transcriptional
T9340 22943-22951 NN denotes activity
T9341 22952-22954 IN denotes by
T9342 22955-22963 VBG denotes altering
T9343 22964-22967 DT denotes the
T9344 22968-22979 JJ denotes competitive
T9345 22980-22987 JJ denotes binding
T9346 22988-22990 IN denotes of
T9347 22991-22995 NNP denotes CREB
T9348 22996-22999 CC denotes and
T9349 23000-23005 NNP denotes NF-κB
T9350 23006-23008 TO denotes to
T9351 23009-23012 DT denotes the
T9352 23013-23021 NN denotes promoter
T9353 23022-23029 NNS denotes regions
T9354 23030-23032 IN denotes of
T9355 23033-23049 JJ denotes pro-inflammatory
T9356 23050-23055 NNS denotes genes
T9357 23055-23056 . denotes .
T9358 23057-23063 NNP denotes Herein
T9359 23063-23064 , denotes ,
T9360 23065-23067 PRP denotes we
T9361 23068-23074 VBD denotes showed
T9362 23075-23079 IN denotes that
T9363 23080-23083 DT denotes the
T9364 23084-23094 NN denotes activation
T9365 23095-23098 CC denotes and
T9366 23099-23102 NN denotes DNA
T9367 23103-23110 JJ denotes binding
T9368 23111-23119 NN denotes activity
T9369 23120-23122 IN denotes of
T9370 23123-23127 NNP denotes CREB
T9371 23128-23130 VBZ denotes is
T9372 23131-23142 VBN denotes upregulated
T9373 23143-23145 IN denotes in
T9374 23146-23147 DT denotes a
T9375 23148-23152 NN denotes dose
T9376 23153-23162 JJ denotes dependent
T9377 23163-23169 NN denotes manner
T9378 23170-23172 IN denotes by
T9379 23173-23176 NNP denotes PTX
T9380 23176-23177 . denotes .
T9381 23178-23180 IN denotes Of
T9382 23181-23185 NN denotes note
T9383 23185-23186 , denotes ,
T9384 23187-23190 DT denotes the
T9385 23191-23199 NN denotes increase
T9386 23200-23202 IN denotes in
T9387 23203-23207 NNP denotes CREB
T9388 23208-23216 NN denotes activity
T9389 23217-23220 VBD denotes was
T9390 23221-23229 RB denotes slightly
T9391 23230-23240 VBN denotes alleviated
T9392 23241-23243 IN denotes by
T9393 23244-23247 DT denotes the
T9394 23248-23256 NN denotes addition
T9395 23257-23259 IN denotes of
T9396 23260-23263 NNP denotes LPS
T9397 23263-23264 , denotes ,
T9398 23265-23275 VBG denotes suggesting
T9399 23276-23287 NN denotes competition
T9400 23288-23295 IN denotes between
T9401 23296-23307 JJ denotes PTX-induced
T9402 23308-23323 JJ denotes CREB-associated
T9403 23324-23328 NN denotes gene
T9404 23329-23342 NN denotes transcription
T9405 23343-23346 CC denotes and
T9406 23347-23352 DT denotes those
T9407 23353-23360 NNS denotes factors
T9408 23360-23361 , denotes ,
T9409 23362-23366 JJ denotes such
T9410 23367-23369 IN denotes as
T9411 23370-23375 NNP denotes NF-κB
T9412 23375-23376 , denotes ,
T9413 23377-23382 WDT denotes which
T9414 23383-23386 MD denotes may
T9415 23387-23389 VB denotes be
T9416 23390-23401 VBN denotes upregulated
T9417 23402-23404 IN denotes by
T9418 23405-23408 NNP denotes LPS
T9419 23409-23417 NN denotes exposure
T9420 23417-23418 . denotes .
T9421 23419-23423 DT denotes This
T9422 23424-23435 NN denotes competition
T9423 23436-23439 VBZ denotes has
T9424 23440-23444 VBN denotes been
T9425 23445-23455 VBN denotes postulated
T9426 23456-23458 TO denotes to
T9427 23459-23466 VB denotes involve
T9428 23467-23470 DT denotes the
T9429 23471-23482 NN denotes recruitment
T9430 23483-23485 IN denotes of
T9431 23486-23489 DT denotes the
T9432 23490-23502 NN denotes co-activator
T9433 23503-23506 NNP denotes CBP
T9434 23507-23510 CC denotes and
T9435 23511-23514 PRP$ denotes its
T9436 23515-23524 NN denotes homologue
T9437 23525-23531 CD denotes p30034
T9438 23531-23532 , denotes ,
T9439 23533-23543 VBG denotes suggesting
T9440 23544-23546 DT denotes an
T9441 23547-23558 JJ denotes alternative
T9442 23559-23568 NN denotes mechanism
T9443 23569-23573 WDT denotes that
T9444 23574-23577 MD denotes may
T9445 23578-23580 VB denotes be
T9446 23581-23589 VBN denotes involved
T9447 23590-23592 IN denotes in
T9448 23593-23596 DT denotes the
T9449 23597-23614 JJ denotes anti-inflammatory
T9450 23615-23622 NNS denotes actions
T9451 23623-23625 IN denotes of
T9452 23626-23629 NNP denotes PTX
T9453 23629-23630 . denotes .
T9454 23631-23637 JJ denotes Future
T9455 23638-23645 NNS denotes studies
T9456 23646-23649 VBP denotes are
T9457 23650-23657 VBN denotes planned
T9458 23658-23660 TO denotes to
T9459 23661-23670 VB denotes delineate
T9460 23671-23674 DT denotes the
T9461 23675-23683 NN denotes validity
T9462 23684-23686 IN denotes of
T9463 23687-23691 DT denotes this
T9464 23692-23701 JJ denotes potential
T9465 23702-23711 NN denotes mechanism
T9466 23711-23712 . denotes .
T9467 23713-23716 DT denotes The
T9468 23717-23724 NNS denotes effects
T9469 23725-23727 IN denotes of
T9470 23728-23731 NNP denotes PTX
T9471 23732-23734 IN denotes on
T9472 23735-23740 JJ denotes other
T9473 23741-23754 NN denotes transcription
T9474 23755-23762 NNS denotes factors
T9475 23762-23763 , denotes ,
T9476 23764-23768 JJ denotes such
T9477 23769-23771 IN denotes as
T9478 23772-23776 NN denotes AP-1
T9479 23776-23777 , denotes ,
T9480 23778-23783 NNS denotes c-fos
T9481 23783-23784 , denotes ,
T9482 23785-23788 CC denotes and
T9483 23789-23794 JJ denotes c-jun
T9484 23794-23795 , denotes ,
T9485 23796-23799 MD denotes can
T9486 23799-23802 RB denotes not
T9487 23803-23805 VB denotes be
T9488 23806-23811 VBN denotes ruled
T9489 23812-23815 RP denotes out
T9490 23816-23818 IN denotes by
T9491 23819-23822 PRP$ denotes our
T9492 23823-23834 NNS denotes experiments
T9493 23834-23835 . denotes .
T9494 23836-23846 NNP denotes Activation
T9495 23847-23849 IN denotes of
T9496 23850-23854 NNP denotes AP-1
T9497 23855-23858 CC denotes and
T9498 23859-23862 PRP$ denotes its
T9499 23863-23873 NNS denotes components
T9500 23874-23875 -LRB- denotes (
T9501 23875-23880 NNS denotes c-fos
T9502 23881-23884 CC denotes and
T9503 23885-23890 JJ denotes c-jun
T9504 23890-23891 -RRB- denotes )
T9505 23892-23894 IN denotes by
T9506 23895-23901 NNS denotes agents
T9507 23902-23906 WDT denotes that
T9508 23907-23914 VBP denotes enhance
T9509 23915-23926 JJ denotes mononuclear
T9510 23927-23931 NN denotes cell
T9511 23932-23940 NN denotes activity
T9512 23941-23944 VBZ denotes has
T9513 23945-23949 VBN denotes been
T9514 23950-23960 VBN denotes implicated
T9515 23961-23963 IN denotes in
T9516 23964-23969 JJ denotes TNF-α
T9517 23970-23980 NN denotes expression
T9518 23980-23981 . denotes .
T9519 23982-23990 RB denotes Moreover
T9520 23990-23991 , denotes ,
T9521 23992-23996 NNP denotes AP-1
T9522 23997-24000 CC denotes and
T9523 24001-24005 NNP denotes CREB
T9524 24006-24015 VBP denotes recognize
T9525 24016-24017 DT denotes a
T9526 24018-24025 JJ denotes similar
T9527 24026-24029 NNP denotes DNA
T9528 24030-24037 JJ denotes binding
T9529 24038-24046 NN denotes sequence
T9530 24047-24049 IN denotes in
T9531 24050-24053 DT denotes the
T9532 24054-24062 NN denotes promoter
T9533 24063-24069 NN denotes region
T9534 24070-24072 IN denotes of
T9535 24073-24076 DT denotes the
T9536 24077-24082 NNP denotes TNF-α
T9537 24083-24089 NNS denotes gene35
T9538 24089-24090 . denotes .
T9539 24091-24096 VBN denotes Given
T9540 24097-24102 DT denotes these
T9541 24103-24116 NNS denotes relationships
T9542 24116-24117 , denotes ,
T9543 24118-24120 PRP denotes it
T9544 24121-24123 VBZ denotes is
T9545 24124-24135 JJ denotes conceivable
T9546 24136-24140 IN denotes that
T9547 24141-24144 NNP denotes PTX
T9548 24145-24148 MD denotes may
T9549 24149-24154 VB denotes exert
T9550 24155-24159 DT denotes some
T9551 24160-24162 IN denotes of
T9552 24163-24166 PRP$ denotes its
T9553 24167-24184 JJ denotes anti-inflammatory
T9554 24185-24192 NNS denotes actions
T9555 24193-24195 IN denotes by
T9556 24196-24205 VBG denotes affecting
T9557 24206-24210 JJ denotes AP-1
T9558 24211-24221 NN denotes activation
T9559 24222-24225 CC denotes and
T9560 24226-24234 VBG denotes favoring
T9561 24235-24239 NNP denotes CREB
T9562 24240-24247 JJ denotes binding
T9563 24247-24248 , denotes ,
T9564 24249-24253 RB denotes thus
T9565 24254-24264 VBG denotes inhibiting
T9566 24265-24270 JJ denotes TNF-α
T9567 24271-24275 NN denotes gene
T9568 24276-24289 NN denotes transcription
T9569 24289-24290 . denotes .
T9570 24291-24301 JJ denotes Additional
T9571 24302-24309 NNS denotes studies
T9572 24310-24313 VBP denotes are
T9573 24314-24323 JJ denotes necessary
T9574 24324-24326 TO denotes to
T9575 24327-24336 VB denotes elucidate
T9576 24337-24340 DT denotes the
T9577 24341-24348 JJ denotes complex
T9578 24349-24361 NNS denotes interactions
T9579 24362-24369 IN denotes between
T9580 24370-24374 NNP denotes CREB
T9581 24374-24375 , denotes ,
T9582 24376-24381 NNP denotes NF-κB
T9583 24381-24382 , denotes ,
T9584 24383-24386 CC denotes and
T9585 24387-24391 NNP denotes AP-1
T9586 24392-24401 VBG denotes following
T9587 24402-24405 NNP denotes LPS
T9588 24406-24414 NN denotes exposure
T9589 24415-24418 CC denotes and
T9590 24419-24422 NNP denotes PDE
T9591 24423-24433 NN denotes inhibition
T9592 24433-24434 . denotes .
T9593 24435-24446 RB denotes Furthermore
T9594 24446-24447 , denotes ,
T9595 24448-24451 DT denotes the
T9596 24452-24458 NN denotes effect
T9597 24459-24461 IN denotes of
T9598 24462-24465 NNP denotes PTX
T9599 24466-24468 IN denotes on
T9600 24469-24472 DT denotes the
T9601 24473-24477 NNP denotes cGMP
T9602 24478-24485 NN denotes pathway
T9603 24486-24491 IN denotes after
T9604 24492-24495 NNP denotes LPS
T9605 24496-24507 NN denotes stimulation
T9606 24508-24511 VBZ denotes has
T9607 24512-24515 RB denotes not
T9608 24516-24520 VBN denotes been
T9609 24521-24529 VBN denotes explored
T9610 24529-24530 , denotes ,
T9611 24531-24539 IN denotes although
T9612 24540-24542 PRP denotes we
T9613 24543-24547 VBP denotes plan
T9614 24548-24550 TO denotes to
T9615 24551-24553 VB denotes do
T9616 24554-24556 RB denotes so
T9617 24557-24559 IN denotes in
T9618 24560-24563 DT denotes the
T9619 24564-24570 NN denotes future
T9620 24570-24571 . denotes .
T9621 24572-24574 IN denotes In
T9622 24575-24582 NN denotes summary
T9623 24582-24583 , denotes ,
T9624 24584-24586 PRP denotes we
T9625 24587-24591 VBP denotes have
T9626 24592-24604 VBN denotes demonstrated
T9627 24605-24609 IN denotes that
T9628 24610-24613 NNP denotes PDE
T9629 24614-24624 NN denotes inhibition
T9630 24625-24627 IN denotes of
T9631 24628-24633 JJ denotes human
T9632 24634-24645 NN denotes mononuclear
T9633 24646-24651 NNS denotes cells
T9634 24652-24665 VBZ denotes downregulates
T9635 24666-24671 JJ denotes TNF-α
T9636 24672-24682 NN denotes production
T9637 24682-24683 , denotes ,
T9638 24684-24686 IN denotes at
T9639 24687-24692 JJS denotes least
T9640 24693-24695 IN denotes in
T9641 24696-24700 NN denotes part
T9642 24700-24701 , denotes ,
T9643 24702-24709 IN denotes through
T9644 24710-24711 DT denotes a
T9645 24712-24727 JJ denotes PKA-independent
T9646 24728-24737 NN denotes mechanism
T9647 24737-24738 . denotes .
T9648 24739-24741 IN denotes In
T9649 24742-24750 NN denotes addition
T9650 24750-24751 , denotes ,
T9651 24752-24755 NNP denotes PTX
T9652 24756-24766 VBZ denotes attenuates
T9653 24767-24770 DT denotes the
T9654 24771-24779 NN denotes activity
T9655 24780-24782 IN denotes of
T9656 24783-24787 NN denotes NF-B
T9657 24788-24793 IN denotes while
T9658 24794-24806 VBG denotes upregulating
T9659 24807-24811 NNP denotes CREB
T9660 24812-24822 NN denotes activation
T9661 24823-24828 IN denotes after
T9662 24829-24832 NNP denotes LPS
T9663 24833-24844 NN denotes stimulation
T9664 24844-24845 , denotes ,
T9665 24846-24851 WDT denotes which
T9666 24852-24855 MD denotes may
T9667 24856-24862 VB denotes result
T9668 24863-24865 IN denotes in
T9669 24866-24876 NN denotes modulation
T9670 24877-24879 IN denotes of
T9671 24880-24896 JJ denotes pro-inflammatory
T9672 24897-24905 NN denotes mediator
T9673 24906-24915 NN denotes synthesis
T9674 24915-24916 . denotes .
T9675 24917-24926 RB denotes Therefore
T9676 24926-24927 , denotes ,
T9677 24928-24931 NNP denotes PTX
T9678 24932-24935 MD denotes may
T9679 24936-24941 VB denotes serve
T9680 24942-24944 IN denotes as
T9681 24945-24946 DT denotes a
T9682 24947-24956 JJ denotes potential
T9683 24957-24964 NN denotes adjunct
T9684 24965-24976 JJ denotes therapeutic
T9685 24977-24980 IN denotes for
T9686 24981-24984 DT denotes the
T9687 24985-24994 NN denotes treatment
T9688 24995-24997 IN denotes of
T9689 24998-25008 NNS denotes conditions
T9690 25009-25011 IN denotes in
T9691 25012-25017 WDT denotes which
T9692 25018-25023 JJ denotes TNF-α
T9693 25024-25034 NN denotes production
T9694 25035-25040 VBZ denotes plays
T9695 25041-25042 DT denotes a
T9696 25043-25054 JJ denotes significant
T9697 25055-25059 NN denotes role
T9698 25059-25060 . denotes .
T130 0-8 NNPS denotes Insights
T131 9-13 IN denotes into
T132 14-17 DT denotes the
T133 18-28 NN denotes Regulation
T134 29-31 IN denotes of
T135 32-37 NNP denotes TNF-α
T136 38-48 NNP denotes Production
T137 49-51 IN denotes in
T138 52-57 NNP denotes Human
T139 58-69 NNP denotes Mononuclear
T140 70-75 NNPS denotes Cells
T141 75-76 : denotes :
T142 77-80 DT denotes The
T143 81-88 NNPS denotes Effects
T144 89-91 IN denotes of
T145 92-104 NNP denotes Non-Specific
T146 105-122 NNP denotes Phosphodiesterase
T147 123-133 NNP denotes Inhibition
T148 145-154 NNP denotes OBJECTIVE
T149 155-158 DT denotes The
T150 159-168 NN denotes objective
T151 169-171 IN denotes of
T152 172-176 DT denotes this
T153 177-182 NN denotes study
T154 183-186 VBD denotes was
T155 187-189 TO denotes to
T156 190-199 VB denotes determine
T157 200-203 DT denotes the
T158 204-210 NN denotes effect
T159 211-213 IN denotes of
T160 214-225 JJ denotes nonspecific
T161 226-243 NN denotes phosphodiesterase
R78 T180 T162 punct -,inhibition
R79 T181 T154 conj stimulated,was
R80 T182 T184 amod human,cells
R81 T183 T184 compound mononuclear,cells
R82 T184 T181 dobj cells,stimulated
R83 T185 T154 punct .,was
R84 T186 T194 advmod INTRODUCTION,is
R85 T187 T188 det The,production
R86 T188 T194 nsubj production,is
R87 T189 T188 prep of,production
R88 T190 T189 pobj TNF-α,of
R89 T191 T188 prep following,production
R90 T192 T193 compound LPS,stimulation
R91 T193 T191 pobj stimulation,following
R92 T194 T194 ROOT is,is
R93 T195 T194 attr one,is
R94 T196 T195 prep of,one
R95 T197 T199 det the,steps
R96 T198 T199 amod key,steps
R97 T199 T196 pobj steps,of
R98 T200 T199 prep in,steps
R99 T201 T202 amod bacterial,sepsis
R103 T205 T194 punct .,is
R104 T206 T207 det The,mechanism
R105 T207 T220 nsubj mechanism,remains
R106 T208 T212 prep by,alters
R107 T209 T208 pobj which,by
R108 T210 T211 amod phosphodiesterase,inhibition
R109 T211 T212 nsubj inhibition,alters
R110 T212 T207 relcl alters,mechanism
R111 T213 T214 amod TNF-α,production
R112 T214 T212 dobj production,alters
R113 T215 T212 prep in,alters
R114 T216 T217 det the,presence
R115 T217 T215 pobj presence,in
R116 T218 T217 prep of,presence
R117 T219 T218 pobj LPS,of
R118 T220 T220 ROOT remains,remains
R119 T221 T220 acomp unclear,remains
R120 T222 T220 punct .,remains
R121 T223 T226 compound METHODS,cells
R122 T224 T226 compound Human,cells
R123 T225 T226 compound mononuclear,cells
R124 T226 T228 nsubjpass cells,stimulated
R125 T227 T228 auxpass were,stimulated
R126 T228 T249 ccomp stimulated,mM
R127 T229 T228 prep with,stimulated
R128 T230 T229 pobj LPS,with
R129 T231 T235 punct (,mL
R130 T232 T233 nummod 1,μg
R131 T233 T235 compound μg,mL
R132 T234 T235 compound /,mL
R133 T235 T230 appos mL,LPS
R134 T236 T235 punct ),mL
R135 T237 T230 punct ",",LPS
R136 T238 T228 prep in,stimulated
R137 T239 T240 det the,presence
R138 T240 T238 pobj presence,in
R139 T241 T240 cc and,presence
R140 T242 T240 conj absence,presence
R141 T243 T242 prep of,absence
R142 T244 T243 pobj Pentoxifylline,of
R143 T245 T246 punct (,PTX
R144 T246 T244 appos PTX,Pentoxifylline
R145 T247 T249 punct ;,mM
R146 T248 T249 nummod 20,mM
R147 T249 T249 ROOT mM,mM
R148 T250 T249 punct ),mM
R149 T251 T249 punct ",",mM
R150 T252 T255 det a,inhibitor
R151 T253 T255 amod nonspecific,inhibitor
R152 T254 T255 compound phosphodiesterase,inhibitor
R153 T255 T249 appos inhibitor,mM
R154 T256 T249 punct .,mM
R155 T257 T258 amod Western,blotting
R156 T258 T281 nsubjpass blotting,performed
R157 T259 T258 prep of,blotting
R158 T260 T262 amod phosphorylated,I-κBα
R159 T261 T262 compound cytoplasmic,I-κBα
R160 T262 T259 pobj I-κBα,of
R161 T263 T262 punct ",",I-κBα
R162 T264 T266 amod nuclear,p65
R163 T265 T266 compound factor-κB,p65
R164 T266 T262 appos p65,I-κBα
R165 T267 T266 punct (,p65
R166 T268 T266 appos NF-κB,p65
R167 T269 T266 punct ),p65
R168 T270 T266 punct ",",p65
R169 T271 T266 cc and,p65
R170 T272 T274 amod nuclear,element
R171 T273 T274 amod cAMP-response,element
R172 T274 T276 compound element,protein
R173 T275 T276 amod binding,protein
R174 T276 T266 conj protein,p65
R175 T277 T278 punct (,CREB
R176 T278 T276 appos CREB,protein
R177 T279 T276 punct ),protein
R178 T280 T281 auxpass was,performed
R179 T281 T281 ROOT performed,performed
R180 T282 T281 punct .,performed
R181 T283 T284 npadvmod DNA,binding
R182 T284 T290 nsubjpass binding,verified
R183 T285 T284 prep of,binding
R184 T286 T285 pobj NF-κB,of
R185 T287 T286 cc and,NF-κB
R186 T288 T286 conj CREB,NF-κB
R187 T289 T290 auxpass was,verified
R188 T290 T290 ROOT verified,verified
R189 T291 T290 agent by,verified
R190 T292 T295 amod electrophoretic,assay
R191 T293 T294 compound mobility,shift
R192 T294 T295 compound shift,assay
R193 T295 T291 pobj assay,by
R194 T296 T290 punct .,verified
R195 T297 T298 amod TNF-α,levels
R196 T298 T300 nsubjpass levels,determined
R197 T299 T300 auxpass were,determined
R198 T300 T300 ROOT determined,determined
R199 T301 T300 prep in,determined
R200 T302 T303 det the,supernatant
R201 T303 T301 pobj supernatant,in
R202 T304 T303 prep of,supernatant
R203 T305 T306 amod stimulated,cells
R204 T306 T304 pobj cells,of
R205 T307 T303 prep in,supernatant
R206 T308 T309 det the,presence
R207 T309 T307 pobj presence,in
R208 T310 T309 cc and,presence
R209 T311 T309 conj absence,presence
R210 T312 T313 compound Protein,kinase
R211 T313 T313 ROOT kinase,kinase
R212 T314 T315 det A,inhibition
R213 T315 T315 ROOT inhibition,inhibition
R214 T316 T315 prep by,inhibition
R215 T317 T320 det an,assay
R216 T318 T320 nmod enzyme-linked,assay
R217 T319 T320 compound immunosorbent,assay
R218 T320 T316 pobj assay,by
R219 T321 T320 punct (,assay
R220 T322 T320 appos ELISA,assay
R221 T323 T320 punct ),assay
R222 T324 T315 punct .,inhibition
R223 T325 T326 compound RESULTS,PTX
R224 T326 T328 nsubjpass PTX,demonstrated
R225 T327 T328 auxpass was,demonstrated
R226 T328 T328 ROOT demonstrated,demonstrated
R227 T329 T331 aux to,reduce
R228 T330 T331 advmod significantly,reduce
R229 T331 T328 xcomp reduce,demonstrated
R230 T332 T334 amod cytoplasmic,phosphorylation
R231 T333 T334 amod I-κBα,phosphorylation
R232 T334 T331 dobj phosphorylation,reduce
R233 T335 T334 punct ",",phosphorylation
R234 T336 T337 amod nuclear,p65
R235 T337 T338 compound p65,phosphorylation
R236 T338 T334 appos phosphorylation,phosphorylation
R237 T339 T338 punct ",",phosphorylation
R238 T340 T338 cc and,phosphorylation
R239 T341 T344 det the,activity
R240 T342 T344 nmod DNA,activity
R241 T343 T344 amod binding,activity
R242 T344 T338 conj activity,phosphorylation
R243 T345 T344 prep of,activity
R244 T346 T345 pobj NF-κB,of
R245 T347 T328 punct .,demonstrated
R246 T348 T353 prep In,enhanced
R247 T349 T348 pobj contrast,In
R248 T350 T353 punct ",",enhanced
R249 T351 T353 nsubj PTX,enhanced
R250 T352 T353 advmod markedly,enhanced
R251 T353 T353 ROOT enhanced,enhanced
R252 T354 T355 det the,phosphorylation
R253 T355 T353 dobj phosphorylation,enhanced
R254 T356 T355 cc and,phosphorylation
R255 T357 T359 amod DNA,activity
R256 T358 T359 amod binding,activity
R257 T359 T355 conj activity,phosphorylation
R258 T360 T355 prep of,phosphorylation
R259 T361 T360 pobj CREB,of
R260 T362 T353 punct .,enhanced
R261 T363 T370 nsubj Cells,secreted
R262 T364 T365 advmod concomitantly,treated
R263 T365 T363 acl treated,Cells
R264 T366 T365 prep with,treated
R265 T367 T366 pobj PTX,with
R266 T368 T367 cc and,PTX
R267 T369 T367 conj LPS,PTX
R268 T370 T370 ROOT secreted,secreted
R269 T371 T372 amod similar,levels
R270 T372 T370 dobj levels,secreted
R271 T373 T372 prep of,levels
R272 T374 T373 pobj TNF-α,of
R273 T375 T370 prep in,secreted
R274 T376 T377 det the,presence
R275 T377 T375 pobj presence,in
R276 T378 T377 cc and,presence
R277 T379 T377 conj absence,presence
R278 T380 T381 compound Protein,kinase
R279 T381 T381 ROOT kinase,kinase
R280 T382 T383 det A,inhibition
R281 T383 T383 ROOT inhibition,inhibition
R282 T384 T383 punct .,inhibition
R283 T385 T396 nsubj DISCUSSION,affects
R284 T386 T388 det The,level
R285 T387 T388 amod increased,level
R286 T388 T396 nsubj level,affects
R287 T389 T388 prep of,level
R288 T390 T389 pobj cAMP,of
R289 T391 T392 nsubj that,results
R290 T392 T388 relcl results,level
R291 T393 T392 prep from,results
R292 T394 T395 amod phosphodiesterase,inhibition
R293 T395 T393 pobj inhibition,from
R294 T396 T396 ROOT affects,affects
R295 T397 T400 amod cytoplasmic,events
R296 T398 T397 cc and,cytoplasmic
R297 T399 T397 conj nuclear,cytoplasmic
R298 T400 T396 dobj events,affects
R299 T401 T396 punct ",",affects
R300 T402 T396 advcl resulting,affects
R301 T403 T402 prep in,resulting
R302 T404 T405 det the,attenuation
R303 T405 T403 pobj attenuation,in
R304 T406 T405 prep of,attenuation
R305 T407 T406 pobj NF-κB,of
R306 T408 T405 cc and,attenuation
R307 T409 T410 det the,activation
R308 T410 T405 conj activation,attenuation
R309 T411 T410 prep of,activation
R310 T412 T414 compound CREB,DNA
R311 T413 T414 compound transcriptional,DNA
R312 T414 T411 pobj DNA,of
R313 T415 T410 amod binding,activation
R314 T416 T415 prep through,binding
R315 T417 T416 pobj pathways,through
R316 T418 T419 nsubj that,are
R317 T419 T417 relcl are,pathways
R318 T420 T419 advmod partially,are
R319 T421 T423 compound Protein,A-independent
R320 T422 T423 compound kinase,A-independent
R321 T423 T419 attr A-independent,are
R322 T424 T396 punct .,affects
R323 T425 T428 nmod CONCLUSION,inhibition
R324 T426 T428 amod PTX-mediated,inhibition
R325 T427 T428 compound phosphodiesterase,inhibition
R326 T428 T429 nsubj inhibition,occurs
R327 T429 T429 ROOT occurs,occurs
R328 T430 T431 advmod partially,through
R329 T431 T429 prep through,occurs
R330 T432 T436 det a,pathway
R331 T433 T436 nmod Protein,pathway
R332 T434 T436 nmod kinase,pathway
R333 T435 T436 compound A-independent,pathway
R334 T436 T431 pobj pathway,through
R335 T437 T429 cc and,occurs
R336 T438 T439 aux may,serve
R337 T439 T429 conj serve,occurs
R338 T440 T439 prep as,serve
R348 T450 T429 punct .,occurs
R765 T140 T137 pobj Cells,in
R766 T141 T143 punct :,Effects
R767 T142 T143 det The,Effects
R768 T143 T130 appos Effects,Insights
R769 T144 T143 prep of,Effects
R770 T145 T146 compound Non-Specific,Phosphodiesterase
R771 T146 T148 compound Phosphodiesterase,OBJECTIVE
R772 T147 T148 compound Inhibition,OBJECTIVE
R773 T148 T143 appos OBJECTIVE,Effects
R774 T149 T150 det The,objective
R775 T150 T154 nsubj objective,was
R776 T151 T150 prep of,objective
R777 T152 T153 det this,study
R778 T153 T151 pobj study,of
R779 T154 T154 ROOT was,was
R780 T155 T156 aux to,determine
R781 T156 T154 xcomp determine,was
R782 T157 T158 det the,effect
R783 T158 T156 dobj effect,determine
R784 T159 T158 prep of,effect
R785 T160 T162 amod nonspecific,inhibition
R786 T161 T162 compound phosphodiesterase,inhibition
R848 T1162 T1165 det The,component
R849 T1163 T1165 amod bacterial,component
R850 T1164 T1165 compound membrane,component
R851 T1165 T1170 nsubj component,is
R852 T1166 T1170 nsubj lipopolysaccharide,is
R853 T1167 T1168 punct (,LPS
R854 T1168 T1166 appos LPS,lipopolysaccharide
R855 T1169 T1166 punct ),lipopolysaccharide
R856 T1170 T1170 ROOT is,is
R857 T1171 T1170 acomp capable,is
R858 T1172 T1171 prep of,capable
R859 T1173 T1172 pcomp initiating,of
R860 T1174 T1173 dobj phosphorylation,initiating
R861 T1175 T1174 cc and,phosphorylation
R862 T1176 T1174 conj activation,phosphorylation
R863 T1177 T1174 prep of,phosphorylation
R864 T1178 T1182 amod multiple,kinases
R865 T1179 T1182 nmod host,kinases
R866 T1180 T1182 amod intracellular,kinases
R867 T1181 T1182 compound protein,kinases
R868 T1182 T1177 pobj kinases,of
R869 T1183 T1182 cc and,kinases
R870 T1184 T1185 compound transcription,factors
R871 T1185 T1182 conj factors,kinases
R872 T1186 T1170 punct .,is
R873 T1187 T1188 compound Transcription,factor
R874 T1188 T1189 compound factor,activation
R875 T1189 T1203 nsubj activation,is
R876 T1190 T1189 punct ",",activation
R877 T1191 T1192 nsubj which,results
R878 T1192 T1189 relcl results,activation
R879 T1193 T1192 prep in,results
R880 T1194 T1195 det the,modulation
R881 T1195 T1193 pobj modulation,in
R882 T1196 T1195 prep of,modulation
R883 T1197 T1198 compound gene,transcription
R884 T1198 T1196 pobj transcription,of
R885 T1199 T1198 cc and,transcription
R886 T1200 T1201 compound protein,synthesis
R887 T1201 T1198 conj synthesis,transcription
R888 T1202 T1203 punct ",",is
R889 T1203 T1203 ROOT is,is
R890 T1204 T1206 det a,element
R891 T1205 T1206 amod critical,element
R892 T1206 T1203 attr element,is
R893 T1207 T1206 prep in,element
R894 T1208 T1210 det the,mechanism
R895 T1209 T1210 compound defense,mechanism
R896 T1210 T1207 pobj mechanism,in
R897 T1211 T1210 prep of,mechanism
R898 T1212 T1215 det the,system
R899 T1213 T1215 nmod host,system
R900 T1214 T1215 amod immune,system
R901 T1215 T1211 pobj system,of
R902 T1216 T1220 nummod ".1,2",activation
R903 T1217 T1220 amod LPS-induced,activation
R904 T1218 T1219 compound transcription,factor
R905 T1219 T1220 compound factor,activation
R906 T1220 T1223 nsubjpass activation,shown
R907 T1221 T1223 aux has,shown
R908 T1222 T1223 auxpass been,shown
R909 T1223 T1223 ROOT shown,shown
R910 T1224 T1225 aux to,be
R911 T1225 T1223 xcomp be,shown
R912 T1226 T1228 det a,regulator
R913 T1227 T1228 amod key,regulator
R914 T1228 T1225 attr regulator,be
R915 T1229 T1228 prep of,regulator
R916 T1230 T1231 compound tumor,necrosis
R917 T1231 T1232 compound necrosis,factor-α
R918 T1232 T1229 pobj factor-α,of
R919 T1233 T1234 punct (,TNF-α
R920 T1234 T1232 appos TNF-α,factor-α
R921 T1235 T1232 punct ),factor-α
R922 T1236 T1238 compound production,TNF-α
R923 T1237 T1238 nummod .3,TNF-α
R924 T1238 T1228 appos TNF-α,regulator
R938 T1252 T1203 punct .,is
R939 T1253 T1259 advmod Furthermore,attenuated
R940 T1254 T1259 punct ",",attenuated
R941 T1255 T1256 amod TNF-α,synthesis
R942 T1256 T1259 nsubjpass synthesis,attenuated
R943 T1257 T1259 aux can,attenuated
R944 T1258 T1259 auxpass be,attenuated
R945 T1259 T1259 ROOT attenuated,attenuated
R946 T1260 T1259 prep in,attenuated
R947 T1261 T1262 amod immune,cells
R948 T1262 T1260 pobj cells,in
R949 T1263 T1259 conj exposed,attenuated
R950 T1264 T1265 aux to,phosphodiesterase
R951 T1265 T1263 advcl phosphodiesterase,exposed
R952 T1266 T1267 punct (,PDE
R953 T1267 T1265 appos PDE,phosphodiesterase
R954 T1268 T1259 punct ),attenuated
R955 T1269 T1301 nsubjpass inhibition,characterized
R956 T1270 T1269 prep after,inhibition
R957 T1271 T1270 pobj challenge,after
R958 T1272 T1271 prep by,challenge
R959 T1273 T1274 det a,variety
R960 T1274 T1272 pobj variety,by
R961 T1275 T1274 prep of,variety
R962 T1276 T1277 amod pro-inflammatory,stimulants
R963 T1277 T1275 pobj stimulants,of
R964 T1278 T1271 nummod .4,challenge
R965 T1279 T1281 det The,mechanisms
R966 T1280 T1281 amod signaling,mechanisms
R967 T1281 T1301 nsubjpass mechanisms,characterized
R968 T1282 T1281 acl affected,mechanisms
R969 T1283 T1282 agent by,affected
R970 T1284 T1285 compound PDE,inhibition
R971 T1285 T1283 pobj inhibition,by
R972 T1286 T1285 punct ",",inhibition
R973 T1287 T1289 nsubj which,lead
R974 T1288 T1289 advmod ultimately,lead
R975 T1289 T1285 relcl lead,inhibition
R976 T1290 T1289 prep to,lead
R977 T1291 T1292 det the,downregulation
R978 T1292 T1290 pobj downregulation,to
R979 T1293 T1292 prep of,downregulation
R980 T1294 T1295 compound TNF-α,production
R981 T1295 T1293 pobj production,of
R982 T1296 T1301 punct ",",characterized
R983 T1297 T1301 aux have,characterized
R984 T1298 T1301 neg not,characterized
R985 T1299 T1301 auxpass been,characterized
R986 T1300 T1301 advmod well,characterized
R987 T1301 T1301 ROOT characterized,characterized
R988 T1302 T1301 prep in,characterized
R989 T1303 T1304 amod inflammatory,cells
R990 T1304 T1302 pobj cells,in
R991 T1305 T1301 punct .,characterized
R992 T1306 T1311 advmod Classically,demonstrated
R993 T1307 T1311 punct ",",demonstrated
R994 T1308 T1311 nsubjpass it,demonstrated
R995 T1309 T1311 aux has,demonstrated
R996 T1310 T1311 auxpass been,demonstrated
R997 T1311 T1311 ROOT demonstrated,demonstrated
R998 T1312 T1337 mark that,kinase
R999 T1313 T1315 compound PDE,results
R1000 T1314 T1315 compound inhibition,results
R1001 T1315 T1337 nsubj results,kinase
R1002 T1316 T1315 prep in,results
R1003 T1317 T1319 det the,accumulation
R1004 T1318 T1319 amod intracellular,accumulation
R1005 T1319 T1316 pobj accumulation,in
R1006 T1320 T1319 prep of,accumulation
R1007 T1321 T1323 det the,messenger
R1008 T1322 T1323 amod second,messenger
R1009 T1323 T1320 pobj messenger,of
R1010 T1324 T1328 nmod cyclic,monophosphate
R1011 T1325 T1328 amod adenosine-3,monophosphate
R1012 T1326 T1328 punct ",5",monophosphate
R1013 T1327 T1328 punct -,monophosphate
R1014 T1328 T1315 appos monophosphate,results
R1015 T1329 T1330 punct (,cAMP
R1016 T1330 T1328 appos cAMP,monophosphate
R1017 T1331 T1330 punct ),cAMP
R1018 T1332 T1328 cc and,monophosphate
R1019 T1333 T1334 amod subsequent,activation
R1020 T1334 T1328 conj activation,monophosphate
R1021 T1335 T1334 prep of,activation
R1022 T1336 T1335 pobj Protein,of
R1023 T1337 T1311 ccomp kinase,demonstrated
R1024 T1338 T1340 det A,PKA
R1025 T1339 T1340 punct (,PKA
R1026 T1340 T1337 appos PKA,kinase
R1027 T1341 T1340 punct ),PKA
R1028 T1342 T1344 nummod .5,activation
R1029 T1343 T1344 compound PKA,activation
R1030 T1344 T1346 nsubj activation,leads
R1031 T1345 T1346 advmod then,leads
R1032 T1346 T1346 ROOT leads,leads
R1033 T1347 T1346 prep to,leads
R1034 T1348 T1349 det the,phosphorylation
R1035 T1349 T1347 pobj phosphorylation,to
R1036 T1350 T1349 prep of,phosphorylation
R1037 T1351 T1353 det the,factor
R1038 T1352 T1353 compound transcription,factor
R1039 T1353 T1350 pobj factor,of
R1040 T1354 T1355 amod cAMP-response,element
R1041 T1355 T1365 npadvmod element,into
R1042 T1356 T1357 amod binding,protein
R1043 T1357 T1355 appos protein,element
R1044 T1358 T1359 punct (,CREB
R1045 T1359 T1357 appos CREB,protein
R1046 T1360 T1359 punct ),CREB
R1047 T1361 T1357 punct ",",protein
R1048 T1362 T1357 conj transmission,protein
R1049 T1363 T1362 prep of,transmission
R1050 T1364 T1363 pobj signals,of
R1051 T1365 T1349 prep into,phosphorylation
R1052 T1366 T1367 det the,nucleus
R1053 T1367 T1365 pobj nucleus,into
R1054 T1368 T1346 punct ",",leads
R1055 T1369 T1346 cc and,leads
R1056 T1370 T1372 det the,modulation
R1057 T1371 T1372 amod subsequent,modulation
R1058 T1372 T1346 conj modulation,leads
R1059 T1373 T1372 prep of,modulation
R1060 T1374 T1375 compound gene,transcription
R1061 T1375 T1373 pobj transcription,of
R1062 T1376 T1375 nummod .6,transcription
R1063 T1377 T1381 det This,cascade
R1064 T1378 T1379 advmod apparently,simple
R1065 T1379 T1381 amod simple,cascade
R1066 T1380 T1381 amod linear,cascade
R1067 T1381 T1385 nsubj cascade,explain
R1068 T1382 T1385 aux does,explain
R1069 T1383 T1385 neg not,explain
R1070 T1384 T1385 advmod fully,explain
R1071 T1385 T1385 ROOT explain,explain
R1072 T1386 T1387 det the,mechanism
R1073 T1387 T1385 dobj mechanism,explain
R1074 T1388 T1397 prep by,exerts
R1075 T1389 T1388 pobj which,by
R1076 T1390 T1391 det an,elevation
R1077 T1391 T1397 nsubj elevation,exerts
R1078 T1392 T1391 prep in,elevation
R1079 T1393 T1396 det the,level
R1080 T1394 T1396 amod intracellular,level
R1081 T1395 T1396 compound cAMP,level
R1082 T1396 T1392 pobj level,in
R1083 T1397 T1387 relcl exerts,mechanism
R1084 T1398 T1399 amod wide-ranging,effects
R1085 T1399 T1397 dobj effects,exerts
R1086 T1400 T1399 prep on,effects
R1087 T1401 T1403 amod multiple,functions
R1088 T1402 T1403 amod cellular,functions
R1089 T1403 T1400 pobj functions,on
R1090 T1404 T1385 punct .,explain
R1091 T1405 T1406 expl There,is
R1092 T1406 T1406 ROOT is,is
R1093 T1407 T1409 det a,body
R1094 T1408 T1409 amod growing,body
R1100 T1414 T1416 nsubj cAMP,function
R1101 T1415 T1416 aux may,function
R1102 T1416 T1412 ccomp function,suggesting
R1103 T1417 T1416 prep through,function
R1104 T1418 T1419 preconj both,PKA-dependent
R1105 T1419 T1423 amod PKA-dependent,mechanisms
R1106 T1420 T1419 cc and,PKA-dependent
R1107 T1421 T1419 punct -,PKA-dependent
R1108 T1422 T1423 amod independent,mechanisms
R1109 T1423 T1417 pobj mechanisms,through
R1110 T1424 T1423 appos .6,mechanisms
R1111 T1425 T1427 nummod 8,activation
R1112 T1426 T1427 amod LPS-induced,activation
R1113 T1427 T1439 nsubj activation,been
R1114 T1428 T1427 prep of,activation
R1115 T1429 T1431 det the,factor
R1116 T1430 T1431 compound transcription,factor
R1117 T1431 T1428 pobj factor,of
R1118 T1432 T1433 amod nuclear,factor-κB
R1119 T1433 T1439 nsubj factor-κB,been
R1120 T1434 T1435 punct (,NF-κB
R1121 T1435 T1433 appos NF-κB,factor-κB
R1122 T1436 T1433 punct ),factor-κB
R1123 T1437 T1439 aux has,been
R1124 T1438 T1439 advmod also,been
R1125 T1439 T1406 conj been,is
R1126 T1440 T1441 det the,focus
R1127 T1441 T1439 attr focus,been
R1128 T1442 T1441 prep of,focus
R1129 T1443 T1445 det a,deal
R1130 T1444 T1445 amod great,deal
R1131 T1445 T1442 pobj deal,of
R1132 T1446 T1445 prep of,deal
R1133 T1447 T1446 pobj research,of
R1134 T1448 T1439 punct .,been
R1135 T1449 T1453 nsubjpass It,demonstrated
R1136 T1450 T1453 aux has,demonstrated
R1137 T1451 T1453 auxpass been,demonstrated
R1138 T1452 T1453 advmod clearly,demonstrated
R1139 T1453 T1453 ROOT demonstrated,demonstrated
R1140 T1454 T1461 mark that,inhibit
R1141 T1455 T1461 nsubj agents,inhibit
R1142 T1456 T1457 nsubj that,increase
R1143 T1457 T1455 relcl increase,agents
R1144 T1458 T1459 compound intracellular,cAMP
R1145 T1459 T1457 dobj cAMP,increase
R1146 T1460 T1461 advmod also,inhibit
R1147 T1461 T1453 ccomp inhibit,demonstrated
R1148 T1462 T1465 amod NF-κB-dependent,transcription
R1149 T1463 T1465 amod pro-inflammatory,transcription
R1150 T1464 T1465 compound gene,transcription
R1151 T1465 T1461 dobj transcription,inhibit
R1152 T1466 T1465 punct ",",transcription
R1153 T1467 T1468 advmod particularly,of
R1154 T1468 T1465 prep of,transcription
R1155 T1469 T1471 det the,gene
R1156 T1470 T1471 compound TNF-α,gene
R1157 T1471 T1468 pobj gene,of
R1158 T1472 T1473 nummod .9,Controversy
R1159 T1473 T1474 nsubj Controversy,exists
R1160 T1474 T1465 relcl exists,transcription
R1161 T1475 T1474 prep regarding,exists
R1162 T1476 T1478 det the,mechanism
R1163 T1477 T1478 amod exact,mechanism
R1164 T1478 T1475 pobj mechanism,regarding
R1165 T1479 T1478 punct (,mechanism
R1166 T1480 T1478 appos s,mechanism
R1167 T1481 T1478 punct ),mechanism
R1168 T1482 T1486 prep by,down-regulates
R1169 T1483 T1482 pobj which,by
R1170 T1484 T1486 nmod PDE,down-regulates
R1171 T1485 T1486 compound inhibition,down-regulates
R1172 T1486 T1474 conj down-regulates,exists
R1173 T1487 T1488 amod TNF-α,production
R1174 T1488 T1486 dobj production,down-regulates
R1175 T1489 T1453 punct .,demonstrated
R1176 T1490 T1491 nsubj Possibilities,include
R1177 T1491 T1491 ROOT include,include
R1178 T1492 T1491 punct ",",include
R1179 T1493 T1491 cc but,include
R1180 T1494 T1496 auxpass are,limited
R1181 T1495 T1496 neg not,limited
R1182 T1496 T1491 conj limited,include
R1183 T1497 T1496 prep to,limited
R1184 T1498 T1497 punct ",",to
R1185 T1499 T1497 pobj inhibition,to
R1186 T1500 T1499 prep of,inhibition
R1187 T1501 T1502 compound NF-κB,DNA
R1188 T1502 T1500 pobj DNA,of
R1189 T1503 T1504 amod binding,activity
R1190 T1504 T1499 conj activity,inhibition
R1191 T1505 T1499 punct ",",inhibition
R1192 T1506 T1499 appos downregulation,inhibition
R1193 T1507 T1506 prep of,downregulation
R1194 T1508 T1510 amod NF-κB,activity
R1195 T1509 T1510 amod transcriptional,activity
R1196 T1510 T1507 pobj activity,of
R1197 T1511 T1512 punct ",",increased
R1198 T1512 T1514 amod increased,activation
R1199 T1513 T1514 compound CREB,activation
R1200 T1514 T1496 dobj activation,limited
R1201 T1515 T1514 punct ",",activation
R1202 T1516 T1514 cc and,activation
R1203 T1517 T1514 conj competition,activation
R1204 T1518 T1514 prep between,activation
R1205 T1519 T1518 pobj NF-κB,between
R1206 T1520 T1519 cc and,NF-κB
R1207 T1521 T1519 conj CREB,NF-κB
R1208 T1522 T1514 prep for,activation
R1209 T1523 T1524 amod common,co-activators
R1210 T1524 T1522 pobj co-activators,for
R1211 T1525 T1526 amod such,as
R1212 T1526 T1524 prep as,co-activators
R1213 T1527 T1526 pobj CREB,as
R1214 T1528 T1529 amod binding,protein
R1215 T1529 T1527 appos protein,CREB
R1216 T1530 T1531 punct (,CBP
R1217 T1531 T1529 appos CBP,protein
R1218 T1532 T1527 punct ),CREB
R1219 T1533 T1491 punct .,include
R1220 T1534 T1535 nsubj It,is
R1221 T1535 T1538 auxpass is,known
R1222 T1536 T1535 advmod also,is
R1223 T1537 T1538 neg not,known
R1224 T1538 T1538 ROOT known,known
R1225 T1539 T1542 mark whether,rely
R1226 T1540 T1541 det these,processes
R1227 T1541 T1542 nsubj processes,rely
R1228 T1542 T1538 ccomp rely,known
R1229 T1543 T1544 advmod solely,upon
R1230 T1544 T1542 prep upon,rely
R1231 T1545 T1546 det the,activation
R1232 T1546 T1544 pobj activation,upon
R1233 T1547 T1546 prep of,activation
R1234 T1548 T1547 pobj PKA,of
R1235 T1549 T1538 punct .,known
R1236 T1550 T1558 advmod Therefore,is
R1237 T1551 T1558 punct ",",is
R1238 T1552 T1553 det the,objective
R1239 T1553 T1558 nsubj objective,is
R1240 T1554 T1553 prep of,objective
R1241 T1555 T1557 det the,study
R1242 T1556 T1557 amod present,study
R1243 T1557 T1554 pobj study,of
R1244 T1558 T1558 ROOT is,is
R1245 T1559 T1560 aux to,determine
R1246 T1560 T1558 xcomp determine,is
R1247 T1561 T1562 det the,effects
R1248 T1562 T1560 dobj effects,determine
R1249 T1563 T1562 prep of,effects
R1250 T1564 T1566 amod nonspecific,inhibition
R1251 T1565 T1566 compound PDE,inhibition
R1252 T1566 T1563 pobj inhibition,of
R1253 T1567 T1562 prep with,effects
R1254 T1568 T1570 meta 1,[
R1255 T1569 T1570 punct -,[
R1256 T1570 T1572 compound [,]
R1257 T1571 T1572 compound 5-oxohexyl,]
R1258 T1572 T1567 pobj ],with
R1259 T1573 T1575 nummod "-3,7",dimethylxanthine
R1260 T1574 T1575 punct -,dimethylxanthine
R1261 T1575 T1572 appos dimethylxanthine,]
R1262 T1576 T1577 punct (,Pentoxifylline
R1263 T1577 T1575 dep Pentoxifylline,dimethylxanthine
R1264 T1578 T1577 punct ;,Pentoxifylline
R1265 T1579 T1577 appos PTX,Pentoxifylline
R1266 T1580 T1577 punct ),Pentoxifylline
R1267 T1581 T1577 prep on,Pentoxifylline
R1268 T1582 T1581 pobj NF-κB,on
R1269 T1583 T1582 cc and,NF-κB
R1270 T1584 T1585 compound CREB,activation
R1271 T1585 T1582 conj activation,NF-κB
R1272 T1586 T1585 prep in,activation
R1273 T1587 T1586 pobj vitro,in
R1274 T1588 T1585 prep in,activation
R1275 T1589 T1591 amod human,cells
R1276 T1590 T1591 compound mononuclear,cells
R1277 T1591 T1588 pobj cells,in
R1278 T1592 T1558 punct .,is
R1279 T1593 T1602 prep With,investigated
R1280 T1594 T1595 det the,use
R1281 T1595 T1593 pobj use,With
R1282 T1596 T1595 prep of,use
R1283 T1597 T1598 amod specific,inhibitors
R1284 T1598 T1596 pobj inhibitors,of
R1285 T1599 T1602 punct ",",investigated
R1286 T1600 T1602 nsubj we,investigated
R1287 T1601 T1602 advmod also,investigated
R1288 T1602 T1602 ROOT investigated,investigated
R1289 T1603 T1604 det the,role
R1290 T1604 T1602 dobj role,investigated
R1291 T1605 T1604 prep of,role
R1292 T1606 T1605 pobj PKA,of
R1293 T1607 T1604 prep in,role
R1294 T1608 T1610 amod LPS-induced,production
R1295 T1609 T1610 compound TNF-α,production
R1296 T1610 T1607 pobj production,in
R1297 T1611 T1602 punct .,investigated
R1891 T2464 T2465 det this,study
R1892 T2465 T2467 nsubjpass study,approved
R1893 T2466 T2467 auxpass was,approved
R1894 T2467 T2467 ROOT approved,approved
R1895 T2468 T2467 agent by,approved
R1896 T2469 T2473 det the,Program
R1897 T2470 T2472 compound Human,Protections
R1898 T2471 T2472 compound Research,Protections
R1899 T2472 T2473 compound Protections,Program
R1900 T2473 T2468 pobj Program,by
R1901 T2474 T2473 cc and,Program
R1902 T2475 T2478 det the,Board
R1903 T2476 T2478 compound Institutional,Board
R1904 T2477 T2478 compound Review,Board
R1905 T2478 T2473 conj Board,Program
R1906 T2479 T2467 punct .,approved
R1907 T2480 T2481 amod Written,consent
R1908 T2481 T2488 nsubjpass consent,obtained
R1909 T2482 T2483 aux to,participate
R1910 T2483 T2481 relcl participate,consent
R1911 T2484 T2483 prep in,participate
R1912 T2485 T2486 det the,study
R1913 T2486 T2484 pobj study,in
R1914 T2487 T2488 auxpass was,obtained
R1915 T2488 T2488 ROOT obtained,obtained
R1916 T2489 T2488 prep from,obtained
R1917 T2490 T2491 det all,volunteers
R1918 T2491 T2489 pobj volunteers,from
R1919 T2492 T2488 advmod prior,obtained
R1920 T2493 T2492 prep to,prior
R1921 T2494 T2493 pobj blood,to
R1922 T2495 T2496 amod "donation. Heparinized",Vacutainers
R1923 T2496 T2493 pobj Vacutainers,to
R1924 T2497 T2498 auxpass were,purchased
R1925 T2498 T2488 conj purchased,obtained
R1926 T2499 T2498 prep from,purchased
R1927 T2500 T2501 compound Becton,Dickinson
R1928 T2501 T2499 pobj Dickinson,from
R1929 T2502 T2501 punct (,Dickinson
R1930 T2503 T2504 compound San,Jose
R1931 T2504 T2501 appos Jose,Dickinson
R1932 T2505 T2504 punct ",",Jose
R1933 T2506 T2501 conj CA,Dickinson
R1934 T2507 T2488 punct ),obtained
R1935 T2508 T2488 punct .,obtained
R1936 T2509 T2512 nummod 1.5-mL,tubes
R1937 T2510 T2512 compound Eppendorf,tubes
R1938 T2511 T2512 compound centrifuge,tubes
R1939 T2512 T2514 nsubjpass tubes,purchased
R1940 T2513 T2514 auxpass were,purchased
R1941 T2514 T2514 ROOT purchased,purchased
R1942 T2515 T2514 prep from,purchased
R1943 T2516 T2517 compound Fisher,Scientific
R1944 T2517 T2515 pobj Scientific,from
R1945 T2518 T2517 punct (,Scientific
R1946 T2519 T2517 appos Pittsburg,Scientific
R1947 T2520 T2519 punct ",",Pittsburg
R1948 T2521 T2517 conj PA,Scientific
R1949 T2522 T2514 punct ),purchased
R1950 T2523 T2514 punct .,purchased
R1951 T2524 T2527 poss Hank,solution
R1952 T2525 T2527 amod balanced,solution
R1953 T2526 T2527 compound salt,solution
R1954 T2527 T2535 nsubjpass solution,obtained
R1955 T2528 T2529 punct (,HBSS
R1956 T2529 T2527 appos HBSS,solution
R1957 T2530 T2529 punct ),HBSS
R1958 T2531 T2527 cc and,solution
R1959 T2532 T2533 compound RPMI,1640
R1960 T2533 T2535 nsubjpass 1640,obtained
R1961 T2534 T2535 auxpass were,obtained
R1962 T2535 T2535 ROOT obtained,obtained
R1963 T2536 T2535 prep from,obtained
R1964 T2537 T2538 compound Irvine,Scientific
R1965 T2538 T2536 pobj Scientific,from
R1966 T2539 T2538 punct (,Scientific
R1967 T2540 T2541 compound Santa,Ana
R1968 T2541 T2538 appos Ana,Scientific
R1969 T2542 T2541 punct ",",Ana
R1970 T2543 T2538 conj CA,Scientific
R1971 T2544 T2535 punct ),obtained
R1972 T2545 T2535 punct .,obtained
R1973 T2546 T2548 compound LPS,coli serotype
R1974 T2547 T2548 compound from Escherichia,coli serotype
R1975 T2548 T2555 nsubjpass coli serotype,purchased
R1976 T2549 T2548 nummod 0111,coli serotype
R1977 T2550 T2548 punct :,coli serotype
R1978 T2551 T2548 appos B4,coli serotype
R1979 T2552 T2551 cc and,B4
R1980 T2553 T2551 conj PTX,B4
R1981 T2554 T2555 auxpass were,purchased
R1982 T2555 T2555 ROOT purchased,purchased
R1983 T2556 T2555 prep from,purchased
R1984 T2557 T2556 pobj Sigma,from
R1985 T2558 T2557 punct (,Sigma
R1986 T2559 T2560 compound St.,Louis
R1987 T2560 T2557 appos Louis,Sigma
R1988 T2561 T2557 punct ",",Sigma
R1989 T2562 T2557 appos MO,Sigma
R1990 T2563 T2557 punct ),Sigma
R1991 T2564 T2555 punct .,purchased
R1992 T2565 T2567 det The,inhibitor
R1993 T2566 T2567 compound PKA,inhibitor
R1994 T2567 T2591 nsubjpass inhibitor,purchased
R1995 T2568 T2567 punct ",",inhibitor
R1996 T2569 T2571 compound N,[
R1997 T2570 T2571 punct -,[
R1998 T2571 T2581 compound [,]
R1999 T2572 T2576 meta 2,p-bromocinnamyl
R2000 T2573 T2576 punct -,p-bromocinnamyl
R2001 T2574 T2576 punct (,p-bromocinnamyl
R2002 T2575 T2576 punct (,p-bromocinnamyl
R2003 T2576 T2581 nmod p-bromocinnamyl,]
R2004 T2577 T2576 punct ),p-bromocinnamyl
R2005 T2578 T2576 amod amino,p-bromocinnamyl
R2006 T2579 T2576 punct ),p-bromocinnamyl
R2007 T2580 T2581 compound ethyl,]
R2008 T2581 T2567 appos ],inhibitor
R2009 T2582 T2584 punct -5,isoguinolinesulfonamide-2HCl
R2010 T2583 T2584 punct -,isoguinolinesulfonamide-2HCl
R2011 T2584 T2585 compound isoguinolinesulfonamide-2HCl,]
R2012 T2585 T2581 appos ],]
R2013 T2586 T2587 punct (,H89
R2014 T2587 T2585 appos H89,]
R2015 T2588 T2585 punct ),]
R2016 T2589 T2591 punct ",",purchased
R2017 T2590 T2591 auxpass was,purchased
R2018 T2591 T2591 ROOT purchased,purchased
R2019 T2592 T2591 prep from,purchased
R2020 T2593 T2592 pobj Calbiochem,from
R2021 T2594 T2593 punct (,Calbiochem
R2022 T2595 T2596 compound La,Jolla
R2023 T2596 T2593 appos Jolla,Calbiochem
R2024 T2597 T2596 punct ",",Jolla
R2025 T2598 T2596 appos CA,Jolla
R2026 T2599 T2593 punct ),Calbiochem
R2027 T2600 T2591 punct .,purchased
R2028 T2601 T2632 nsubjpass Antibodies,purchased
R2029 T2602 T2601 prep for,Antibodies
R2030 T2603 T2604 compound phosphorylated,CREB
R2031 T2604 T2602 pobj CREB,for
R2032 T2605 T2606 punct (,serine
R2033 T2606 T2604 appos serine,CREB
R2034 T2607 T2606 nummod 133,serine
R2035 T2608 T2606 punct ),serine
R2036 T2609 T2604 punct ",",CREB
R2037 T2610 T2611 compound phosphorylated,I-κBα
R2038 T2611 T2604 conj I-κBα,CREB
R2039 T2612 T2613 punct (,serines
R2040 T2613 T2611 parataxis serines,I-κBα
R2041 T2614 T2613 nummod 32,serines
R2042 T2615 T2614 cc and,32
R2043 T2616 T2614 conj 36,32
R2044 T2617 T2613 punct ),serines
R2045 T2618 T2632 punct ",",purchased
R2046 T2619 T2620 compound phosphorylated,NF-κB
R2047 T2620 T2632 nsubjpass NF-κB,purchased
R2048 T2621 T2622 nummod p65,subunit
R2049 T2622 T2620 appos subunit,NF-κB
R2050 T2623 T2624 punct (,serine
R2051 T2624 T2622 appos serine,subunit
R2052 T2625 T2624 nummod 276,serine
R2053 T2626 T2624 punct ),serine
R2054 T2627 T2624 punct ",",serine
R2055 T2628 T2624 cc and,serine
R2056 T2629 T2630 amod secondary,antibodies
R2057 T2630 T2624 conj antibodies,serine
R2058 T2631 T2632 auxpass were,purchased
R2059 T2632 T2632 ROOT purchased,purchased
R2060 T2633 T2632 prep from,purchased
R2061 T2634 T2635 compound Cell,Signaling
R2062 T2635 T2633 pobj Signaling,from
R2063 T2636 T2637 punct (,Beverly
R2064 T2637 T2635 appos Beverly,Signaling
R2065 T2638 T2637 punct ",",Beverly
R2066 T2639 T2637 appos MA,Beverly
R2067 T2640 T2637 punct ),Beverly
R2068 T2641 T2632 punct .,purchased
R2069 T2642 T2642 ROOT Dextran,Dextran
R2070 T2643 T2642 nummod T500,Dextran
R2071 T2644 T2647 cc and,received
R2072 T2645 T2647 nsubjpass Percoll,received
R2073 T2646 T2647 auxpass were,received
R2074 T2647 T2647 ROOT received,received
R2075 T2648 T2647 prep from,received
R2076 T2649 T2651 compound GE,Biosciences
R2077 T2650 T2651 compound Healthcare/Amersham,Biosciences
R2078 T2651 T2648 pobj Biosciences,from
R2079 T2652 T2651 punct (,Biosciences
R2080 T2653 T2651 npadvmod Piscataway,Biosciences
R2081 T2654 T2653 punct ",",Piscataway
R2082 T2655 T2653 appos NJ,Piscataway
R2083 T2656 T2653 punct ),Piscataway
R2084 T2657 T2647 punct .,received
R2085 T2658 T2661 det The,assay
R2086 T2659 T2661 nmod enzyme-linked,assay
R2087 T2660 T2661 compound immunosorbent,assay
R2088 T2661 T2668 nsubjpass assay,obtained
R2089 T2662 T2661 punct (,assay
R2090 T2663 T2661 appos ELISA,assay
R2091 T2664 T2661 punct ),assay
R2092 T2665 T2668 prep for,obtained
R2093 T2666 T2665 pobj TNF-α,for
R2094 T2667 T2668 auxpass was,obtained
R2095 T2668 T2668 ROOT obtained,obtained
R2096 T2669 T2668 prep from,obtained
R2097 T2670 T2672 compound Quantikine,Systems
R2098 T2671 T2672 compound R&D,Systems
R2099 T2672 T2669 pobj Systems,from
R2100 T2673 T2672 punct (,Systems
R2101 T2674 T2672 npadvmod Minneapolis,Systems
R2102 T2675 T2674 punct ",",Minneapolis
R2103 T2676 T2674 appos MN,Minneapolis
R2104 T2677 T2674 punct ),Minneapolis
R2105 T2678 T2668 punct .,obtained
R2106 T2679 T2683 nummod Tris-glycine,gels
R2107 T2680 T2679 cc and,Tris-glycine
R2108 T2681 T2679 conj DNA,Tris-glycine
R2109 T2682 T2683 compound retardation,gels
R2110 T2683 T2694 nsubjpass gels,obtained
R2111 T2684 T2686 punct ",",membranes
R2112 T2685 T2686 amod nitrocellulose,membranes
R2113 T2686 T2683 conj membranes,gels
R2114 T2687 T2686 punct ",",membranes
R2115 T2688 T2686 cc and,membranes
R2116 T2689 T2690 amod western,blot
R2117 T2690 T2692 compound blot,buffers
R2118 T2691 T2692 compound running,buffers
R2119 T2692 T2686 conj buffers,membranes
R2120 T2693 T2694 auxpass were,obtained
R2121 T2694 T2694 ROOT obtained,obtained
R2122 T2695 T2694 prep from,obtained
R2123 T2696 T2695 pobj Invitrogen,from
R2124 T2697 T2696 punct (,Invitrogen
R2125 T2698 T2696 appos Carlsbad,Invitrogen
R2126 T2699 T2698 punct ",",Carlsbad
R2127 T2700 T2698 conj CA,Carlsbad
R2128 T2701 T2696 punct ),Invitrogen
R2129 T2702 T2694 punct .,obtained
R2130 T2703 T2704 compound NE-PER,®
R2131 T2704 T2709 nmod ®,reagents
R2132 T2705 T2709 amod nuclear,reagents
R2133 T2706 T2705 cc and,nuclear
R2134 T2707 T2705 conj cytoplasmic,nuclear
R2135 T2708 T2709 compound extraction,reagents
R2136 T2709 T2731 nsubjpass reagents,purchased
R2137 T2710 T2715 punct ",",kit
R2138 T2711 T2713 det the,protein
R2139 T2712 T2713 compound BCA,protein
R2140 T2713 T2715 compound protein,kit
R2141 T2714 T2715 compound reagent,kit
R2142 T2715 T2731 nsubjpass kit,purchased
R2143 T2716 T2715 punct ",",kit
R2144 T2717 T2722 det the,Kit
R2145 T2718 T2722 compound Supersignal,Kit
R2146 T2719 T2720 compound West,Pico
R2147 T2720 T2722 compound Pico,Kit
R2148 T2721 T2722 compound Chemiluminescent,Kit
R2149 T2722 T2715 conj Kit,kit
R2150 T2723 T2722 punct ",",Kit
R2151 T2724 T2722 cc and,Kit
R2152 T2725 T2729 det the,Kit
R2153 T2726 T2728 compound LightShift,EMSA
R2154 T2727 T2728 compound Chemiluminescent,EMSA
R2155 T2728 T2729 compound EMSA,Kit
R2156 T2729 T2722 conj Kit,Kit
R2157 T2730 T2731 auxpass were,purchased
R2158 T2731 T2731 ROOT purchased,purchased
R2159 T2732 T2731 prep from,purchased
R2161 T2734 T2735 punct (,Rockland
R2162 T2735 T2733 appos Rockland,Pierce
R2163 T2736 T2733 punct ",",Pierce
R2164 T2737 T2731 prep IL,purchased
R2165 T2738 T2731 punct ),purchased
R2166 T2739 T2731 punct .,purchased
R2167 T2740 T2745 nmod CREB,probes
R2168 T2741 T2740 cc and,CREB
R2169 T2742 T2740 conj NF-κB,CREB
R2170 T2743 T2744 compound DNA,oligonucleotide
R2171 T2744 T2745 compound oligonucleotide,probes
R2172 T2745 T2747 nsubjpass probes,synthesized
R2173 T2746 T2747 auxpass were,synthesized
R2174 T2747 T2747 ROOT synthesized,synthesized
R2175 T2748 T2747 agent by,synthesized
R2176 T2749 T2748 pobj IDT,by
R2177 T2750 T2751 punct (,Coralville
R2178 T2751 T2749 appos Coralville,IDT
R2179 T2752 T2751 punct ",",Coralville
R2180 T2753 T2751 appos IA,Coralville
R2181 T2754 T2751 punct ),Coralville
R2182 T2755 T2747 punct .,synthesized
R2183 T2756 T2757 compound Nylon,membranes
R2184 T2757 T2759 nsubjpass membranes,purchased
R2185 T2758 T2759 auxpass were,purchased
R2186 T2759 T2759 ROOT purchased,purchased
R2187 T2760 T2759 prep from,purchased
R2188 T2761 T2763 compound Roche,Sciences
R2189 T2762 T2763 compound Applied,Sciences
R2190 T2763 T2760 pobj Sciences,from
R2191 T2764 T2763 punct (,Sciences
R2192 T2765 T2763 appos Indianapolis,Sciences
R2193 T2766 T2763 punct ",",Sciences
R2194 T2767 T2759 prep IN,purchased
R2195 T2768 T2767 punct ),IN
R2196 T2769 T2759 punct .,purchased
R2197 T2770 T2773 amod Western,quantification
R2198 T2771 T2772 compound blot,band
R2199 T2772 T2773 compound band,quantification
R2200 T2773 T2775 nsubjpass quantification,performed
R2201 T2774 T2775 auxpass was,performed
R2202 T2775 T2775 ROOT performed,performed
R2203 T2776 T2775 prep with,performed
R2204 T2777 T2781 det the,software
R2205 T2778 T2780 compound UN-SCAN-IT,Digitizing
R2206 T2779 T2780 compound Gel,Digitizing
R2207 T2780 T2781 compound Digitizing,software
R2208 T2781 T2776 pobj software,with
R2209 T2782 T2784 punct (,Scientific
R2210 T2783 T2784 compound Silk,Scientific
R2211 T2784 T2781 appos Scientific,software
R2212 T2785 T2784 punct ",",Scientific
R2213 T2786 T2784 appos Orem,Scientific
R2214 T2787 T2786 punct ",",Orem
R2215 T2788 T2786 appos UT,Orem
R2216 T2789 T2786 punct ),Orem
R2217 T2790 T2775 punct .,performed
R2600 T3267 T3268 compound Human,Peripheral
R2601 T3268 T3269 compound Peripheral,Blood
R2602 T3269 T3272 compound Blood,Isolation
R2603 T3270 T3272 compound Mononuclear,Isolation
R2604 T3271 T3272 compound Cell,Isolation
R2605 T3272 T3279 nsubjpass Isolation,isolated
R2606 T3273 T3272 cc and,Isolation
R2607 T3274 T3275 compound Stimulation,Human
R2608 T3275 T3277 compound Human,cells
R2609 T3276 T3277 compound mononuclear,cells
R2610 T3277 T3272 conj cells,Isolation
R2611 T3278 T3279 auxpass were,isolated
R2612 T3279 T3279 ROOT isolated,isolated
R2613 T3280 T3279 prep from,isolated
R2614 T3281 T3283 det the,blood
R2615 T3282 T3283 compound peripheral,blood
R2616 T3283 T3280 pobj blood,from
R2617 T3284 T3283 prep of,blood
R2618 T3285 T3288 nummod four,volunteers
R2619 T3286 T3288 amod healthy,volunteers
R2620 T3287 T3288 amod human,volunteers
R2621 T3288 T3284 pobj volunteers,of
R2622 T3289 T3279 punct .,isolated
R2623 T3290 T3292 det The,size
R2624 T3291 T3292 compound sample,size
R2625 T3292 T3294 nsubjpass size,chosen
R2626 T3293 T3294 auxpass was,chosen
R2627 T3294 T3294 ROOT chosen,chosen
R2628 T3295 T3294 prep from,chosen
R2629 T3296 T3299 poss our,work
R2630 T3297 T3298 advmod previously,published
R2631 T3298 T3299 amod published,work
R2632 T3299 T3295 pobj work,from
R2633 T3300 T3299 punct ",",work
R2634 T3301 T3302 nsubj which,demonstrated
R2635 T3302 T3299 relcl demonstrated,work
R2636 T3303 T3304 amod reliable,results
R2637 T3304 T3302 dobj results,demonstrated
R2638 T3305 T3307 nummod .10,isolation
R2639 T3306 T3307 compound Cell,isolation
R2640 T3307 T3313 nsubjpass isolation,conducted
R2641 T3308 T3307 cc and,isolation
R2642 T3309 T3311 det all,experiments
R2643 T3310 T3311 amod subsequent,experiments
R2644 T3311 T3313 nsubjpass experiments,conducted
R2645 T3312 T3313 auxpass were,conducted
R2646 T3313 T3294 advcl conducted,chosen
R2647 T3314 T3313 prep under,conducted
R2648 T3315 T3318 amod sterile,conditions
R2649 T3316 T3315 cc and,sterile
R2650 T3317 T3315 conj pyrogen-free,sterile
R2651 T3318 T3314 pobj conditions,under
R2652 T3319 T3313 punct .,conducted
R2653 T3320 T3321 nsubj Blood,collected
R2654 T3321 T3321 ROOT collected,collected
R2655 T3322 T3321 prep in,collected
R2656 T3323 T3324 compound heparin,tubes
R2657 T3324 T3322 pobj tubes,in
R2658 T3325 T3326 auxpass was,incubated
R2659 T3326 T3321 conj incubated,collected
R2660 T3327 T3335 mark with,sedimented
R2661 T3328 T3335 nsubjpass Dextran,sedimented
R2662 T3329 T3328 nummod T500,Dextran
R2663 T3330 T3328 cc and,Dextran
R2664 T3331 T3333 det the,cells
R2665 T3332 T3333 amod red,cells
R2666 T3333 T3328 conj cells,Dextran
R2667 T3334 T3335 auxpass were,sedimented
R2668 T3335 T3326 advcl sedimented,incubated
R2669 T3336 T3335 prep for,sedimented
R2670 T3337 T3338 nummod 40,minutes
R2671 T3338 T3336 pobj minutes,for
R2672 T3339 T3338 prep at,minutes
R2673 T3340 T3341 compound room,temperature
R2674 T3341 T3339 pobj temperature,at
R2675 T3342 T3321 punct .,collected
R2676 T3343 T3345 det The,serum
R2677 T3344 T3345 amod resultant,serum
R2678 T3345 T3348 nsubjpass serum,washed
R2679 T3346 T3348 auxpass was,washed
R2680 T3347 T3348 advmod then,washed
R2681 T3348 T3348 ROOT washed,washed
R2682 T3349 T3348 prep with,washed
R2683 T3350 T3349 pobj HBSS,with
R2684 T3351 T3348 cc and,washed
R2685 T3352 T3348 conj separated,washed
R2686 T3353 T3352 agent by,separated
R2687 T3354 T3356 nmod Percoll,centrifugation
R2688 T3355 T3356 compound gradient,centrifugation
R2689 T3356 T3353 pobj centrifugation,by
R2690 T3357 T3348 prep according,washed
R2691 T3358 T3357 prep to,according
R2692 T3359 T3360 det the,manufacturer
R2693 T3360 T3361 poss manufacturer,instructions
R2694 T3361 T3358 pobj instructions,to
R2695 T3362 T3348 punct .,washed
R2696 T3363 T3364 compound Cell,viability
R2697 T3364 T3366 nsubjpass viability,assessed
R2698 T3365 T3366 auxpass was,assessed
R2699 T3366 T3366 ROOT assessed,assessed
R2700 T3367 T3366 agent by,assessed
R2701 T3368 T3371 compound trypan,exclusion
R2702 T3369 T3370 amod blue,dye
R2703 T3370 T3371 compound dye,exclusion
R2704 T3371 T3367 pobj exclusion,by
R2705 T3372 T3371 punct ",",exclusion
R2706 T3373 T3366 prep with,assessed
R2707 T3374 T3373 pobj purity,with
R2708 T3375 T3374 prep of,purity
R2709 T3376 T3378 amod greater,95
R2710 T3377 T3378 quantmod than,95
R2711 T3378 T3379 nummod 95,%
R2712 T3379 T3375 pobj %,of
R2713 T3380 T3366 punct .,assessed
R2714 T3381 T3382 amod Isolated,cells
R2715 T3382 T3384 nsubjpass cells,resuspended
R2716 T3383 T3384 auxpass were,resuspended
R2717 T3384 T3384 ROOT resuspended,resuspended
R2718 T3385 T3384 prep in,resuspended
R2719 T3386 T3388 nsubj RPMI,supplemented
R2720 T3387 T3386 nummod 1640,RPMI
R2721 T3388 T3384 conj supplemented,resuspended
R2722 T3389 T3388 prep with,supplemented
R2723 T3390 T3391 nummod 10,%
R2724 T3391 T3392 compound %,FBS
R2725 T3392 T3389 pobj FBS,with
R2726 T3393 T3392 cc and,FBS
R2727 T3394 T3396 nummod 5,HEPES
R2728 T3395 T3396 compound mM,HEPES
R2729 T3396 T3392 conj HEPES,FBS
R2730 T3397 T3388 prep at,supplemented
R2731 T3398 T3399 det a,concentration
R2732 T3399 T3397 pobj concentration,at
R2733 T3400 T3399 prep of,concentration
R2734 T3401 T3403 nummod 1,107cells/mL
R2735 T3402 T3403 compound x,107cells/mL
R2736 T3403 T3400 pobj 107cells/mL,of
R2737 T3404 T3388 punct .,supplemented
R2738 T3405 T3407 det Each,experiment
R2739 T3406 T3407 amod subsequent,experiment
R2740 T3407 T3408 nsubj experiment,listed
R2741 T3408 T3408 ROOT listed,listed
R2742 T3409 T3408 advmod below,listed
R2743 T3410 T3411 auxpass was,conducted
R2744 T3411 T3408 dep conducted,listed
R2745 T3412 T3411 prep on,conducted
R2746 T3413 T3412 pobj samples,on
R2747 T3414 T3413 prep of,samples
R2748 T3415 T3418 nummod 5,cells
R2749 T3416 T3418 nmod x,cells
R2750 T3417 T3418 nummod 106,cells
R2751 T3418 T3414 pobj cells,of
R2752 T3419 T3418 prep per,cells
R2753 T3420 T3421 compound treatment,group
R2754 T3421 T3419 pobj group,per
R2755 T3422 T3408 punct .,listed
R2756 T3423 T3424 amod Isolated,cells
R2757 T3424 T3426 nsubjpass cells,stimulated
R2758 T3425 T3426 auxpass were,stimulated
R2759 T3426 T3426 ROOT stimulated,stimulated
R2760 T3427 T3426 prep with,stimulated
R2761 T3428 T3429 det either,HBSS
R2762 T3429 T3427 pobj HBSS,with
R2763 T3430 T3429 prep as,HBSS
R2764 T3431 T3433 det a,control
R2765 T3432 T3433 amod negative,control
R2766 T3433 T3430 pobj control,as
R2767 T3434 T3433 punct ",",control
R2768 T3435 T3433 appos LPS,control
R2769 T3436 T3440 punct (,mL
R2770 T3437 T3438 nummod 1,μg
R2771 T3438 T3440 compound μg,mL
R2772 T3439 T3440 compound /,mL
R2773 T3440 T3435 appos mL,LPS
R2774 T3441 T3440 punct ),mL
R2775 T3442 T3435 punct ",",LPS
R2776 T3443 T3454 csubj PTX,","
R2777 T3444 T3446 punct (,mM
R2778 T3445 T3446 nummod 20,mM
R2779 T3446 T3443 parataxis mM,PTX
R2780 T3447 T3446 punct ),mM
R2781 T3448 T3446 punct ",",mM
R2782 T3449 T3443 cc or,PTX
R2783 T3450 T3452 amod concomitant,/
R2784 T3451 T3452 compound LPS,/
R2785 T3452 T3453 compound /,PTX
R2786 T3453 T3443 conj PTX,PTX
R2787 T3454 T3435 punct ",",LPS
R2788 T3455 T3433 prep at,control
R2789 T3456 T3458 det the,concentrations
R2790 T3457 T3458 amod above-mentioned,concentrations
R2791 T3458 T3455 pobj concentrations,at
R2792 T3459 T3426 punct ",",stimulated
R2793 T3460 T3426 prep for,stimulated
R2794 T3461 T3462 nummod 30,minutes
R2795 T3462 T3460 pobj minutes,for
R2796 T3463 T3462 prep at,minutes
R2797 T3464 T3463 pobj 37ºC,at
R2798 T3465 T3426 punct .,stimulated
R2799 T3466 T3468 nsubjpass Cells,placed
R2800 T3467 T3468 auxpass were,placed
R2801 T3468 T3468 ROOT placed,placed
R2802 T3469 T3468 prep on,placed
R2803 T3470 T3469 pobj ice,on
R2804 T3471 T3468 prep for,placed
R2805 T3472 T3473 nummod 10,minutes
R2806 T3473 T3471 pobj minutes,for
R2807 T3474 T3475 aux to,stop
R2808 T3475 T3468 advcl stop,placed
R2809 T3476 T3477 det the,reaction
R2810 T3477 T3475 dobj reaction,stop
R2811 T3478 T3477 cc and,reaction
R2812 T3479 T3480 det the,samples
R2813 T3480 T3483 nsubjpass samples,stored
R2814 T3481 T3483 auxpass were,stored
R2815 T3482 T3483 advmod then,stored
R2816 T3483 T3468 conj stored,placed
R2817 T3484 T3483 prep at,stored
R2818 T3485 T3488 nummod −,C
R2819 T3486 T3487 nummod 70,°
R2820 T3487 T3488 compound °,C
R2821 T3488 T3484 pobj C,at
R2822 T3489 T3483 prep for,stored
R2823 T3490 T3491 amod further,analysis
R2824 T3491 T3489 pobj analysis,for
R2825 T3492 T3483 punct .,stored
R2826 T3493 T3495 det The,times
R2827 T3494 T3495 compound stimulation,times
R2828 T3495 T3502 nsubj times,utilized
R2829 T3496 T3495 cc and,times
R2830 T3497 T3495 conj concentrations,times
R2831 T3498 T3497 prep of,concentrations
R2832 T3499 T3498 pobj LPS,of
R2833 T3500 T3499 cc and,LPS
R2834 T3501 T3499 conj PTX,LPS
R2835 T3502 T3502 ROOT utilized,utilized
R2836 T3503 T3502 prep in,utilized
R2837 T3504 T3505 det this,study
R2838 T3505 T3503 pobj study,in
R2839 T3506 T3507 auxpass were,determined
R2840 T3507 T3502 conj determined,utilized
R2841 T3508 T3507 prep in,determined
R2842 T3509 T3511 amod previous,studies
R2843 T3510 T3511 compound pilot,studies
R2844 T3511 T3508 pobj studies,in
R2845 T3512 T3511 acl done,studies
R2846 T3513 T3512 prep in,done
R2847 T3514 T3515 poss our,laboratory
R2848 T3515 T3513 pobj laboratory,in
R2849 T3516 T3515 punct ",",laboratory
R2850 T3517 T3518 nsubj which,examined
R2851 T3518 T3511 relcl examined,studies
R2852 T3519 T3520 det the,effects
R2853 T3520 T3518 dobj effects,examined
R2854 T3521 T3520 prep of,effects
R2855 T3522 T3521 pcomp increasing,of
R2856 T3523 T3522 dobj concentrations,increasing
R2857 T3524 T3523 prep of,concentrations
R2858 T3525 T3524 pobj PTX,of
R2859 T3526 T3523 prep on,concentrations
R2860 T3527 T3529 amod LPS-induced,production
R2861 T3528 T3529 compound TNF-α,production
R2862 T3529 T3526 pobj production,on
R2863 T3530 T3502 punct .,utilized
R2864 T3531 T3548 nsubj LPS,were
R2865 T3532 T3531 prep at,LPS
R2866 T3533 T3534 det a,concentration
R2867 T3534 T3532 pobj concentration,at
R2868 T3535 T3534 prep of,concentration
R2869 T3536 T3537 nummod 1,μg
R2870 T3537 T3539 compound μg,mL
R2871 T3538 T3539 compound /,mL
R2872 T3539 T3535 pobj mL,of
R2873 T3540 T3539 cc and,mL
R2874 T3541 T3539 conj PTX,mL
R2875 T3542 T3534 prep at,concentration
R2876 T3543 T3544 det a,concentration
R2877 T3544 T3542 pobj concentration,at
R2878 T3545 T3544 prep of,concentration
R2879 T3546 T3547 nummod 20,mM
R2880 T3547 T3545 pobj mM,of
R2881 T3548 T3548 ROOT were,were
R2882 T3549 T3551 det the,concentrations
R2883 T3550 T3551 amod minimum,concentrations
R2884 T3551 T3548 attr concentrations,were
R2885 T3552 T3551 amod necessary,concentrations
R2886 T3553 T3554 aux to,produce
R2887 T3554 T3552 xcomp produce,necessary
R2888 T3555 T3559 det a,up-regulation
R2889 T3556 T3559 amod reproducible,up-regulation
R2890 T3557 T3556 cc and,reproducible
R2891 T3558 T3556 conj reliable,reproducible
R2892 T3559 T3554 dobj up-regulation,produce
R2893 T3560 T3559 cc and,up-regulation
R2894 T3561 T3559 conj down-regulation,up-regulation
R2895 T3562 T3559 prep in,up-regulation
R2896 T3563 T3564 compound TNF-α,production
R2897 T3564 T3562 pobj production,in
R2898 T3565 T3559 punct ",",up-regulation
R2899 T3566 T3554 advmod respectively,produce
R2900 T3567 T3554 punct ",",produce
R2901 T3568 T3554 prep in,produce
R2902 T3569 T3570 amod quantitative,assays
R2903 T3570 T3568 pobj assays,in
R2904 T3571 T3574 punct (,shown
R2905 T3572 T3574 nsubjpass data,shown
R2906 T3573 T3574 neg not,shown
R2907 T3574 T3548 parataxis shown,were
R2908 T3575 T3574 punct ),shown
R2909 T3576 T3548 punct .,were
R3250 T3971 T3971 ROOT Effect,Effect
R3251 T3972 T3971 prep of,Effect
R3252 T3973 T3972 pobj PTX,of
R3253 T3974 T3971 prep on,Effect
R3254 T3975 T3977 amod LPS-induced,production
R3255 T3976 T3977 compound TNF-α,production
R3256 T3977 T3974 pobj production,on
R3257 T3978 T3977 prep by,production
R3258 T3979 T3980 compound mononuclear,cells
R3259 T3980 T3978 pobj cells,by
R3260 T3981 T3982 det The,concentration
R3261 T3982 T3987 nsubjpass concentration,determined
R3262 T3983 T3982 prep of,concentration
R3263 T3984 T3983 pobj TNF-α,of
R3264 T3985 T3987 auxpass was,determined
R3265 T3986 T3987 advmod quantitatively,determined
R3266 T3987 T3987 ROOT determined,determined
R3267 T3988 T3987 agent by,determined
R3268 T3989 T3988 pobj ELISA,by
R3269 T3990 T3987 prep in,determined
R3270 T3991 T3992 det the,media
R3271 T3992 T3990 pobj media,in
R3272 T3993 T3992 prep of,media
R3273 T3994 T3993 pobj cells,of
R3274 T3995 T3994 acl exposed,cells
R3275 T3996 T3995 prep to,exposed
R3276 T3997 T3998 det the,treatments
R3277 T3998 T3996 pobj treatments,to
R3278 T3999 T3998 acl described,treatments
R3279 T4000 T3999 advmod above,described
R3280 T4001 T3999 prep after,described
R3281 T4002 T4001 pobj stimulation,after
R3282 T4003 T3999 prep for,described
R3283 T4004 T4007 nmod up,maximum
R3284 T4005 T4007 quantmod to,maximum
R3285 T4006 T4007 det a,maximum
R3286 T4007 T4003 pobj maximum,for
R3287 T4008 T4007 prep of,maximum
R3288 T4009 T4010 nummod 18,hours
R3289 T4010 T4008 pobj hours,of
R3290 T4011 T3999 punct ",",described
R3291 T4012 T3999 prep at,described
R3292 T4013 T4014 amod 3-hour,intervals
R3293 T4014 T4012 pobj intervals,at
R3294 T4015 T3987 punct .,determined
R3295 T4016 T4017 det The,results
R3296 T4017 T4019 nsubjpass results,expressed
R3297 T4018 T4019 auxpass are,expressed
R3298 T4019 T4019 ROOT expressed,expressed
R3299 T4020 T4019 prep in,expressed
R3300 T4021 T4020 pobj pg/mL,in
R3301 T4022 T4019 punct .,expressed
R3373 T4152 T4152 ROOT SDS-PAGE,SDS-PAGE
R3374 T4153 T4152 cc and,SDS-PAGE
R3375 T4154 T4152 conj Immunoblotting,SDS-PAGE
R3376 T4155 T4156 punct (,I-κBα
R3377 T4156 T4152 conj I-κBα,SDS-PAGE
R3378 T4157 T4156 punct ",",I-κBα
R3379 T4158 T4156 conj NF-κB,I-κBα
R3380 T4159 T4156 appos p65,I-κBα
R3381 T4160 T4156 punct ",",I-κBα
R3382 T4161 T4156 cc and,I-κBα
R3383 T4162 T4156 conj CREB,I-κBα
R3384 T4163 T4156 punct ),I-κBα
R3385 T4164 T4169 prep Following,washed
R3386 T4165 T4164 pobj stimulation,Following
R3387 T4166 T4169 punct ",",washed
R3388 T4167 T4169 nsubjpass PBMCs,washed
R3389 T4168 T4169 auxpass were,washed
R3390 T4169 T4169 ROOT washed,washed
R3391 T4170 T4169 prep with,washed
R3392 T4171 T4172 amod ice-cold,PBS
R3393 T4172 T4170 pobj PBS,with
R3394 T4173 T4169 cc and,washed
R3395 T4174 T4169 conj centrifuged,washed
R3396 T4175 T4176 aux to,collect
R3397 T4176 T4174 advcl collect,centrifuged
R3398 T4177 T4179 det a,pellet
R3399 T4178 T4179 compound cell,pellet
R3400 T4179 T4176 dobj pellet,collect
R3401 T4180 T4169 punct .,washed
R3402 T4181 T4182 det The,pellet
R3403 T4182 T4184 nsubjpass pellet,resuspended
R3404 T4183 T4184 auxpass was,resuspended
R3405 T4184 T4184 ROOT resuspended,resuspended
R3406 T4185 T4184 prep in,resuspended
R3407 T4186 T4189 amod ice-cold,buffer
R3408 T4187 T4189 compound SDS,buffer
R3409 T4188 T4189 compound sample,buffer
R3410 T4189 T4185 pobj buffer,in
R3411 T4190 T4184 conj supplemented,resuspended
R3412 T4191 T4190 prep with,supplemented
R3413 T4192 T4194 nummod 100,dithiothreitol
R3414 T4193 T4194 compound mM,dithiothreitol
R3415 T4194 T4191 pobj dithiothreitol,with
R3416 T4195 T4184 punct .,resuspended
R3417 T4196 T4200 nsubj Cytoplasmic,were
R3418 T4197 T4196 cc and,Cytoplasmic
R3419 T4198 T4199 amod nuclear,extracts
R3420 T4199 T4196 conj extracts,Cytoplasmic
R3421 T4200 T4200 ROOT were,were
R3422 T4201 T4200 acomp isolated,were
R3423 T4202 T4201 prep with,isolated
R3424 T4203 T4208 compound NE-PER,reagents
R3425 T4204 T4207 amod nuclear,extraction
R3426 T4205 T4204 cc and,nuclear
R3427 T4206 T4204 conj cytoplasmic,nuclear
R3428 T4207 T4208 compound extraction,reagents
R3429 T4208 T4202 pobj reagents,with
R3430 T4209 T4208 prep with,reagents
R3431 T4210 T4214 nummod 1x,Cocktail
R3432 T4211 T4214 compound Halt,Cocktail
R3433 T4212 T4214 compound Protease,Cocktail
R3434 T4213 T4214 compound Inhibitor,Cocktail
R3435 T4214 T4215 nsubj Cocktail,according
R3436 T4215 T4200 prep according,were
R3437 T4216 T4215 prep to,according
R3438 T4217 T4218 det the,manufacturer
R3439 T4218 T4219 poss manufacturer,instructions
R3440 T4219 T4216 pobj instructions,to
R3441 T4220 T4200 punct .,were
R3442 T4221 T4222 compound Protein,concentrations
R3443 T4222 T4224 nsubjpass concentrations,determined
R3444 T4223 T4224 auxpass were,determined
R3445 T4224 T4224 ROOT determined,determined
R3446 T4225 T4224 prep with,determined
R3447 T4226 T4228 det the,protein
R3448 T4227 T4228 compound BCA,protein
R3449 T4228 T4230 nmod protein,kit
R3450 T4229 T4230 compound reagent,kit
R3451 T4230 T4225 pobj kit,with
R3452 T4231 T4230 prep for,kit
R3453 T4232 T4233 det each,sample
R3454 T4233 T4231 pobj sample,for
R3455 T4234 T4224 prep according,determined
R3456 T4235 T4234 prep to,according
R3457 T4236 T4238 det a,curve
R3458 T4237 T4238 amod standardized,curve
R3459 T4238 T4235 pobj curve,to
R3460 T4239 T4238 prep for,curve
R3461 T4240 T4239 pobj albumin,for
R3462 T4241 T4224 punct .,determined
R3463 T4242 T4243 compound Cell,lysates
R3464 T4243 T4245 nsubjpass lysates,collected
R3465 T4244 T4245 auxpass were,collected
R3466 T4245 T4245 ROOT collected,collected
R3467 T4246 T4245 prep by,collected
R3468 T4247 T4246 pcomp boiling,by
R3469 T4248 T4249 det the,samples
R3470 T4249 T4247 dobj samples,boiling
R3471 T4250 T4247 prep in,boiling
R3472 T4251 T4254 det a,bath
R3473 T4252 T4254 amod 100ºC,bath
R3474 T4253 T4254 compound water,bath
R3475 T4254 T4250 pobj bath,in
R3476 T4255 T4247 prep for,boiling
R3477 T4256 T4257 nummod 5,minutes
R3478 T4257 T4255 pobj minutes,for
R3479 T4258 T4245 punct .,collected
R3480 T4259 T4260 compound Ten,μg
R3481 T4260 T4266 nsubjpass μg,separated
R3482 T4261 T4260 prep of,μg
R3483 T4262 T4261 pobj protein,of
R3484 T4263 T4262 prep per,protein
R3485 T4264 T4263 pobj sample,per
R3486 T4265 T4266 auxpass was,separated
R3487 T4266 T4266 ROOT separated,separated
R3488 T4267 T4266 agent by,separated
R3489 T4268 T4270 amod SDS-polyacrylamide,electrophoresis
R3490 T4269 T4270 compound gel,electrophoresis
R3491 T4270 T4267 pobj electrophoresis,by
R3492 T4271 T4266 prep through,separated
R3493 T4272 T4271 pobj 8,through
R3494 T4273 T4274 nummod 16,%
R3495 T4274 T4278 nmod %,gels
R3496 T4275 T4278 amod tris-glycine,gels
R3497 T4276 T4278 compound polyacrylamide,gels
R3498 T4277 T4278 compound gradient,gels
R3499 T4278 T4272 appos gels,8
R3500 T4279 T4266 cc and,separated
R3501 T4280 T4266 conj transferred,separated
R3502 T4281 T4280 prep to,transferred
R3503 T4282 T4283 amod nitrocellulose,membranes
R3504 T4283 T4281 pobj membranes,to
R3505 T4284 T4266 punct .,separated
R3506 T4285 T4286 det The,membranes
R3507 T4286 T4288 nsubjpass membranes,blocked
R3508 T4287 T4288 auxpass were,blocked
R3509 T4288 T4288 ROOT blocked,blocked
R3510 T4289 T4288 prep with,blocked
R3511 T4290 T4291 nummod 5,%
R3512 T4291 T4292 compound %,milk
R3513 T4292 T4289 pobj milk,with
R3514 T4293 T4292 prep in,milk
R3515 T4294 T4295 compound Tris-buffered,saline/Tween
R3516 T4295 T4293 pobj saline/Tween,in
R3517 T4296 T4295 nummod 20,saline/Tween
R3518 T4297 T4303 punct (,PA
R3519 T4298 T4299 compound Fischer,Scientific
R3520 T4299 T4303 dep Scientific,PA
R3521 T4300 T4299 punct ",",Scientific
R3522 T4301 T4299 appos Pittsburgh,Scientific
R3523 T4302 T4301 punct ",",Pittsburgh
R3524 T4303 T4303 ROOT PA,PA
R3525 T4304 T4303 punct ),PA
R3526 T4305 T4303 prep for,PA
R3527 T4306 T4307 nummod 1,hour
R3528 T4307 T4305 pobj hour,for
R3529 T4308 T4303 punct .,PA
R3530 T4309 T4310 amod Cytoplasmic,extracts
R3531 T4310 T4312 nsubjpass extracts,incubated
R3532 T4311 T4312 auxpass were,incubated
R3533 T4312 T4312 ROOT incubated,incubated
R3534 T4313 T4312 prep with,incubated
R3535 T4314 T4316 amod phosphorylated,antibody
R3536 T4315 T4316 amod I-κBα,antibody
R3537 T4316 T4313 pobj antibody,with
R3538 T4317 T4316 punct (,antibody
R3539 T4318 T4316 appos 1:200,antibody
R3540 T4319 T4316 punct ),antibody
R3541 T4320 T4312 punct ",",incubated
R3542 T4321 T4325 mark while,incubated
R3543 T4322 T4323 amod nuclear,extracts
R3544 T4323 T4325 nsubjpass extracts,incubated
R3545 T4324 T4325 auxpass were,incubated
R3546 T4325 T4312 advcl incubated,incubated
R3547 T4326 T4325 prep with,incubated
R3548 T4327 T4331 det either,antibody
R3549 T4328 T4331 amod phosphorylated,antibody
R3550 T4329 T4331 nmod NF-κB,antibody
R3551 T4330 T4331 nummod p65,antibody
R3552 T4331 T4326 pobj antibody,with
R3553 T4332 T4331 punct (,antibody
R3554 T4333 T4331 appos 1:500,antibody
R3555 T4334 T4331 punct ),antibody
R3556 T4335 T4325 cc or,incubated
R3557 T4336 T4338 amod phosphorylated,antibody
R3558 T4337 T4338 compound CREB,antibody
R3559 T4338 T4346 nmod antibody,C
R3560 T4339 T4338 punct (,antibody
R3561 T4340 T4338 appos 1:500,antibody
R3562 T4341 T4338 punct ),antibody
R3563 T4342 T4346 amod overnight,C
R3564 T4343 T4342 prep at,overnight
R3565 T4344 T4345 nummod 4,°
R3566 T4345 T4343 pobj °,at
R3567 T4346 T4325 conj C,incubated
R3568 T4347 T4346 prep in,C
R3569 T4348 T4349 amod separate,experiments
R3570 T4349 T4347 pobj experiments,in
R3571 T4350 T4312 punct .,incubated
R3572 T4351 T4352 det The,membranes
R3573 T4352 T4354 nsubjpass membranes,washed
R3574 T4353 T4354 auxpass were,washed
R3575 T4354 T4354 ROOT washed,washed
R3576 T4355 T4354 prep with,washed
R3577 T4356 T4357 compound Tris-buffered,saline/Tween
R3578 T4357 T4355 pobj saline/Tween,with
R3579 T4358 T4357 nummod 20,saline/Tween
R3580 T4359 T4354 cc and,washed
R3581 T4360 T4354 conj incubated,washed
R3582 T4361 T4360 prep for,incubated
R3583 T4362 T4363 nummod 1,hour
R3584 T4363 T4361 pobj hour,for
R3585 T4364 T4360 prep at,incubated
R3586 T4365 T4366 compound room,temperature
R3587 T4366 T4364 pobj temperature,at
R3588 T4367 T4360 prep with,incubated
R3589 T4368 T4370 det the,antibody
R3590 T4369 T4370 amod secondary,antibody
R3591 T4370 T4367 pobj antibody,with
R3592 T4371 T4376 punct ",",diluted
R3593 T4372 T4375 nmod horseradish,IgG
R3594 T4373 T4375 amod peroxidase-linked,IgG
R3595 T4374 T4375 compound anti-rabbit,IgG
R3596 T4375 T4376 nsubj IgG,diluted
R3597 T4376 T4354 conj diluted,washed
R3598 T4377 T4376 dobj 1:2000,diluted
R3599 T4378 T4376 prep in,diluted
R3600 T4379 T4378 pcomp blocking,in
R3601 T4380 T4379 dobj solution,blocking
R3602 T4381 T4376 punct .,diluted
R3603 T4382 T4396 prep After,applied
R3604 T4383 T4384 amod repeated,washing
R3605 T4384 T4382 pobj washing,After
R3606 T4385 T4384 prep of,washing
R3607 T4386 T4387 det the,membrane
R3608 T4387 T4385 pobj membrane,of
R3609 T4388 T4396 punct ",",applied
R3610 T4389 T4394 det the,Kit
R3611 T4390 T4394 compound Supersignal,Kit
R3612 T4391 T4392 compound West,Pico
R3613 T4392 T4394 compound Pico,Kit
R3614 T4393 T4394 compound Chemiluminescent,Kit
R3615 T4394 T4396 nsubjpass Kit,applied
R3616 T4395 T4396 auxpass was,applied
R3617 T4396 T4396 ROOT applied,applied
R3618 T4397 T4396 prep for,applied
R3619 T4398 T4399 compound antibody,detection
R3620 T4399 T4397 pobj detection,for
R3621 T4400 T4399 prep per,detection
R3622 T4401 T4402 det the,manufacturer
R3623 T4402 T4403 poss manufacturer,instructions
R3624 T4403 T4400 pobj instructions,per
R3625 T4404 T4396 punct .,applied
R3626 T4405 T4407 amod Western,data
R3627 T4406 T4407 compound blot,data
R3628 T4407 T4409 nsubjpass data,presented
R3629 T4408 T4409 auxpass is,presented
R3630 T4409 T4409 ROOT presented,presented
R3631 T4410 T4409 prep as,presented
R3632 T4411 T4412 det a,percentage
R3633 T4412 T4410 pobj percentage,as
R3634 T4413 T4412 prep of,percentage
R3635 T4414 T4415 compound LPS,stimulation
R3636 T4415 T4413 pobj stimulation,of
R3637 T4416 T4409 punct .,presented
R3638 T4417 T4418 det The,percentage
R3639 T4418 T4423 nsubjpass percentage,calculated
R3640 T4419 T4418 prep of,percentage
R3641 T4420 T4421 compound LPS,stimulation
R3642 T4421 T4419 pobj stimulation,of
R3643 T4422 T4423 auxpass was,calculated
R3644 T4423 T4423 ROOT calculated,calculated
R3645 T4424 T4423 prep by,calculated
R3646 T4425 T4424 pcomp dividing,by
R3647 T4426 T4430 det the,total
R3648 T4427 T4430 amod mean,total
R3649 T4428 T4429 compound band,pixel
R3650 T4429 T4430 compound pixel,total
R3651 T4430 T4425 dobj total,dividing
R3652 T4431 T4430 prep for,total
R3653 T4432 T4434 det each,arm
R3654 T4433 T4434 compound treatment,arm
R3655 T4434 T4431 pobj arm,for
R3656 T4435 T4430 acl divided,total
R3657 T4436 T4435 agent by,divided
R3658 T4437 T4440 det the,total
R3659 T4438 T4440 amod mean,total
R3660 T4439 T4440 amod pixel,total
R3661 T4440 T4436 pobj total,by
R3662 T4441 T4440 prep of,total
R3663 T4442 T4441 pobj samples,of
R3664 T4443 T4442 acl stimulated,samples
R3665 T4444 T4443 prep with,stimulated
R3666 T4445 T4444 pobj LPS,with
R3667 T4446 T4443 cc and,stimulated
R3668 T4447 T4425 advcl multiplying,dividing
R3669 T4448 T4447 prep by,multiplying
R3670 T4449 T4448 pobj 100,by
R3671 T4450 T4423 punct .,calculated
R3672 T4451 T4456 advmod Thus,reported
R3673 T4452 T4456 punct ",",reported
R3674 T4453 T4454 compound LPS,stimulation
R3675 T4454 T4456 nsubjpass stimulation,reported
R3676 T4455 T4456 auxpass is,reported
R3677 T4456 T4456 ROOT reported,reported
R3678 T4457 T4456 prep as,reported
R3679 T4458 T4459 nummod 100,%
R3680 T4459 T4457 pobj %,as
R3681 T4460 T4456 cc and,reported
R3682 T4461 T4467 nsubjpass each,reported
R3683 T4462 T4461 prep of,each
R3684 T4463 T4465 det the,arms
R3685 T4464 T4465 compound treatment,arms
R3686 T4465 T4462 pobj arms,of
R3687 T4466 T4467 auxpass is,reported
R3688 T4467 T4456 conj reported,reported
R3689 T4468 T4467 prep as,reported
R3690 T4469 T4470 det a,percent
R3691 T4470 T4468 pobj percent,as
R3692 T4471 T4470 prep of,percent
R3693 T4472 T4473 compound LPS,stimulation
R3694 T4473 T4471 pobj stimulation,of
R3695 T4474 T4467 punct .,reported
R4034 T4907 T4907 ROOT NF-κB,NF-κB
R4035 T4908 T4907 cc and,NF-κB
R4036 T4909 T4913 compound CREB,Assay
R4037 T4910 T4913 compound Electrophoretic,Assay
R4038 T4911 T4912 compound Mobility,Shift
R4039 T4912 T4913 compound Shift,Assay
R4040 T4913 T4907 conj Assay,NF-κB
R4041 T4914 T4915 punct (,EMSA
R4042 T4915 T4913 appos EMSA,Assay
R4043 T4916 T4913 punct ),Assay
R4044 T4917 T4922 det The,Kit
R4045 T4918 T4922 amod non-radioactive,Kit
R4046 T4919 T4921 compound LightShift,EMSA
R4047 T4920 T4921 compound Chemiluminescent,EMSA
R4048 T4921 T4922 compound EMSA,Kit
R4049 T4922 T4924 nsubjpass Kit,used
R4050 T4923 T4924 auxpass was,used
R4051 T4924 T4924 ROOT used,used
R4052 T4925 T4926 aux to,detect
R4053 T4926 T4924 xcomp detect,used
R4054 T4927 T4928 amod DNA-protein,interactions
R4055 T4928 T4926 dobj interactions,detect
R4056 T4929 T4924 punct .,used
R4057 T4930 T4934 det The,biotin
R4058 T4931 T4934 nmod NF-κB,biotin
R4059 T4932 T4933 nummod 3,′
R4060 T4933 T4934 compound ′,biotin
R4061 T4934 T4935 nsubj biotin,end-labeled
R4062 T4935 T4935 ROOT end-labeled,end-labeled
R4063 T4936 T4937 compound DNA,oligonucleotide
R4064 T4937 T4945 nsubj oligonucleotide,was
R4065 T4938 T4937 acl used,oligonucleotide
R4066 T4939 T4938 prep as,used
R4067 T4940 T4941 det a,probe
R4068 T4941 T4939 pobj probe,as
R4069 T4942 T4941 prep for,probe
R4070 T4943 T4944 det the,EMSA
R4071 T4944 T4942 pobj EMSA,for
R4072 T4945 T4935 ccomp was,end-labeled
R4073 T4946 T4950 det a,construct
R4074 T4947 T4949 amod 42-bp,stranded
R4075 T4948 T4949 amod double,stranded
R4076 T4949 T4950 amod stranded,construct
R4077 T4950 T4945 attr construct,was
R4078 T4951 T4955 punct (,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4079 T4952 T4955 nummod 5,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4080 T4953 T4955 punct ′,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4081 T4954 T4955 punct -,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4082 T4955 T4959 meta TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC,containing
R4083 T4956 T4957 nummod 3,′
R4084 T4957 T4955 appos ′,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4085 T4958 T4955 punct ),TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4086 T4959 T4950 acl containing,construct
R4087 T4960 T4965 nummod two,sites
R4088 T4961 T4965 amod tandemly,sites
R4089 T4962 T4963 amod repeated,NF-κB
R4090 T4963 T4965 amod NF-κB,sites
R4091 T4964 T4965 amod binding,sites
R4092 T4965 T4959 dobj sites,containing
R4093 T4966 T4965 punct (,sites
R4094 T4967 T4965 acl underlined,sites
R4095 T4968 T4965 punct ),sites
R4096 T4969 T4945 punct .,was
R4097 T4970 T4972 nsubjpass Specificity,determined
R4098 T4971 T4972 auxpass was,determined
R4099 T4972 T4972 ROOT determined,determined
R4100 T4973 T4972 agent by,determined
R4101 T4974 T4976 det a,assay
R4102 T4975 T4976 compound competition,assay
R4103 T4976 T4973 pobj assay,by
R4104 T4977 T4976 prep with,assay
R4105 T4978 T4979 det the,addition
R4106 T4979 T4977 pobj addition,with
R4107 T4980 T4979 prep of,addition
R4108 T4981 T4983 nummod 200,excess
R4109 T4982 T4983 amod molar,excess
R4110 T4983 T4980 pobj excess,of
R4111 T4984 T4983 prep of,excess
R4112 T4985 T4989 amod unlabeled,oligonucleotide
R4113 T4986 T4989 amod double,oligonucleotide
R4114 T4987 T4989 amod stranded,oligonucleotide
R4115 T4988 T4989 compound NF-κB,oligonucleotide
R4116 T4989 T4984 pobj oligonucleotide,of
R4117 T4990 T4972 punct .,determined
R4118 T4991 T4995 det The,biotin
R4119 T4992 T4995 nmod CREB,biotin
R4120 T4993 T4994 nummod 3,′
R4121 T4994 T4995 compound ′,biotin
R4122 T4995 T4996 nsubj biotin,end-labeled
R4123 T4996 T4996 ROOT end-labeled,end-labeled
R4124 T4997 T4998 compound DNA,oligonucleotide
R4125 T4998 T4999 nsubj oligonucleotide,used
R4126 T4999 T4996 conj used,end-labeled
R4127 T5000 T4999 prep as,used
R4128 T5001 T5002 det a,probe
R4129 T5002 T5000 pobj probe,as
R4130 T5003 T5002 prep for,probe
R4131 T5004 T5005 det the,EMSA
R4132 T5005 T5003 pobj EMSA,for
R4133 T5006 T4996 conj was,end-labeled
R4134 T5007 T5011 det a,construct
R4135 T5008 T5010 amod 23-bp,stranded
R4136 T5009 T5010 amod double,stranded
R4137 T5010 T5011 amod stranded,construct
R4138 T5011 T5006 attr construct,was
R4139 T5012 T5019 punct (,TCTGACGTCAGA-GC
R4140 T5013 T5019 nummod 5,TCTGACGTCAGA-GC
R4141 T5014 T5019 punct ′,TCTGACGTCAGA-GC
R4142 T5015 T5019 punct -,TCTGACGTCAGA-GC
R4143 T5016 T5019 compound TTT,TCTGACGTCAGA-GC
R4144 T5017 T5019 compound TCG,TCTGACGTCAGA-GC
R4145 T5018 T5019 compound AGC,TCTGACGTCAGA-GC
R4146 T5019 T5011 appos TCTGACGTCAGA-GC,construct
R4147 T5020 T5021 nummod 3,′
R4148 T5021 T5019 appos ′,TCTGACGTCAGA-GC
R4149 T5022 T5021 punct ),′
R4150 T5023 T5019 acl containing,TCTGACGTCAGA-GC
R4151 T5024 T5027 det the,sequence
R4152 T5025 T5027 compound CRE,sequence
R4153 T5026 T5027 compound consensus,sequence
R4154 T5027 T5023 dobj sequence,containing
R4155 T5028 T5029 punct (,underlined
R4156 T5029 T5019 parataxis underlined,TCTGACGTCAGA-GC
R4157 T5030 T5029 punct ),underlined
R4158 T5031 T4996 punct .,end-labeled
R4159 T5032 T5034 nsubjpass Specificity,determined
R4160 T5033 T5034 auxpass was,determined
R4161 T5034 T5034 ROOT determined,determined
R4162 T5035 T5034 agent by,determined
R4163 T5036 T5038 det a,assay
R4164 T5037 T5038 compound competition,assay
R4165 T5038 T5035 pobj assay,by
R4166 T5039 T5038 prep with,assay
R4167 T5040 T5041 det the,addition
R4168 T5041 T5039 pobj addition,with
R4169 T5042 T5041 prep of,addition
R4170 T5043 T5045 nummod 200,excess
R4171 T5044 T5045 amod molar,excess
R4172 T5045 T5042 pobj excess,of
R4173 T5046 T5045 prep of,excess
R4174 T5047 T5048 amod unlabeled,double
R4175 T5048 T5051 amod double,oligonucleotide
R4176 T5049 T5051 amod stranded,oligonucleotide
R4177 T5050 T5051 compound CREB,oligonucleotide
R4178 T5051 T5046 pobj oligonucleotide,of
R4179 T5052 T5034 punct .,determined
R4180 T5053 T5054 compound Nuclear,extracts
R4181 T5054 T5060 nsubjpass extracts,incubated
R4182 T5055 T5054 punct (,extracts
R4183 T5056 T5057 nummod 10,μg
R4184 T5057 T5054 appos μg,extracts
R4185 T5058 T5054 punct ),extracts
R4186 T5059 T5060 auxpass were,incubated
R4187 T5060 T5060 ROOT incubated,incubated
R4188 T5061 T5060 prep with,incubated
R4189 T5062 T5064 nummod 5,NF-κB
R4190 T5063 T5064 compound nM,NF-κB
R4191 T5064 T5061 pobj NF-κB,with
R4192 T5065 T5064 cc or,NF-κB
R4193 T5066 T5067 compound CREB,probe
R4194 T5067 T5064 conj probe,NF-κB
R4195 T5068 T5150 punct (,electrophoresed
R4196 T5069 T5150 nsubjpass NF-kB,electrophoresed
R4197 T5070 T5069 punct :,NF-kB
R4198 T5071 T5073 nummod 1x,buffer
R4199 T5072 T5073 amod binding,buffer
R4200 T5073 T5069 appos buffer,NF-kB
R4201 T5074 T5073 punct ",",buffer
R4202 T5075 T5077 nummod 50,KCl
R4203 T5076 T5077 compound mM,KCl
R4204 T5077 T5073 appos KCl,buffer
R4205 T5078 T5077 punct ",",KCl
R4206 T5079 T5081 nummod 1,EDTA
R4207 T5080 T5081 compound mM,EDTA
R4208 T5081 T5077 appos EDTA,KCl
R4209 T5082 T5081 punct ",",EDTA
R4210 T5083 T5085 nummod 1,DTT
R4211 T5084 T5085 compound mM,DTT
R4212 T5085 T5081 appos DTT,EDTA
R4213 T5086 T5085 punct ",",DTT
R4214 T5087 T5088 nummod 0.1,%
R4215 T5088 T5085 appos %,DTT
R4216 T5089 T5093 nummod NP40,glycerol
R4217 T5090 T5093 punct ",",glycerol
R4218 T5091 T5092 nummod 10,%
R4219 T5092 T5093 compound %,glycerol
R4220 T5093 T5085 conj glycerol,DTT
R4221 T5094 T5093 punct ",",glycerol
R4222 T5095 T5093 cc and,glycerol
R4223 T5096 T5097 nummod 50,ng
R4224 T5097 T5101 amod ng,dI-dC
R4225 T5098 T5101 nmod /,dI-dC
R4226 T5099 T5101 compound μl,dI-dC
R4227 T5100 T5101 compound poly,dI-dC
R4228 T5101 T5093 conj dI-dC,glycerol
R4229 T5102 T5101 punct ",",dI-dC
R4230 T5103 T5111 nmod CREB,Tris
R4231 T5104 T5107 punct :,buffer
R4232 T5105 T5107 amod 1x,buffer
R4233 T5106 T5107 amod binding,buffer
R4234 T5107 T5111 nmod buffer,Tris
R4235 T5108 T5111 punct ",",Tris
R4236 T5109 T5111 nummod 20,Tris
R4237 T5110 T5111 compound mM,Tris
R4238 T5111 T5101 appos Tris,dI-dC
R4239 T5112 T5111 punct ",",Tris
R4240 T5113 T5111 conj pH,Tris
R4241 T5114 T5113 nummod 7.5,pH
R4242 T5115 T5113 punct ",",pH
R4243 T5116 T5118 nummod 50,KCl
R4244 T5117 T5118 compound mM,KCl
R4245 T5118 T5113 conj KCl,pH
R4246 T5119 T5118 punct ",",KCl
R4247 T5120 T5122 nummod 1,EDTA
R4248 T5121 T5122 compound mM,EDTA
R4249 T5122 T5118 conj EDTA,KCl
R4250 T5123 T5122 punct ",",EDTA
R4251 T5124 T5126 nummod 1,DTT
R4252 T5125 T5126 compound mM,DTT
R4253 T5126 T5122 conj DTT,EDTA
R4254 T5127 T5126 punct ",",DTT
R4255 T5128 T5129 nummod 0.10,%
R4256 T5129 T5126 appos %,DTT
R4257 T5130 T5134 nummod NP40,glycerol
R4258 T5131 T5130 punct ",",NP40
R4259 T5132 T5133 nummod 6,%
R4260 T5133 T5134 compound %,glycerol
R4261 T5134 T5126 conj glycerol,DTT
R4262 T5135 T5134 punct ",",glycerol
R4263 T5136 T5138 nummod 0.1,BSA
R4264 T5137 T5138 compound mg/mL,BSA
R4265 T5138 T5134 conj BSA,glycerol
R4266 T5139 T5138 cc and,BSA
R4267 T5140 T5141 nummod 50,ng
R4268 T5141 T5145 nmod ng,dI-dC
R4269 T5142 T5145 nmod /,dI-dC
R4270 T5143 T5145 nmod μl,dI-dC
R4271 T5144 T5145 compound poly,dI-dC
R4272 T5145 T5138 conj dI-dC,BSA
R4273 T5146 T5145 punct ),dI-dC
R4274 T5147 T5101 cc and,dI-dC
R4275 T5148 T5150 auxpass were,electrophoresed
R4276 T5149 T5150 advmod then,electrophoresed
R4277 T5150 T5150 ROOT electrophoresed,electrophoresed
R4278 T5151 T5150 prep through,electrophoresed
R4279 T5152 T5157 det a,gel
R4280 T5153 T5154 nummod 6,%
R4281 T5154 T5157 compound %,gel
R4282 T5155 T5156 compound DNA,retardation
R4283 T5156 T5157 compound retardation,gel
R4284 T5157 T5151 pobj gel,through
R4285 T5158 T5157 prep at,gel
R4286 T5159 T5158 pobj 100V,at
R4287 T5160 T5150 prep for,electrophoresed
R4288 T5161 T5162 nummod 90,minutes
R4289 T5162 T5160 pobj minutes,for
R4290 T5163 T5150 punct .,electrophoresed
R4291 T5164 T5165 det The,gels
R4292 T5165 T5168 nsubjpass gels,transferred
R4293 T5166 T5168 auxpass were,transferred
R4294 T5167 T5168 advmod electrophoretically,transferred
R4295 T5168 T5168 ROOT transferred,transferred
R4296 T5169 T5168 prep at,transferred
R4297 T5170 T5169 pobj 380mA,at
R4298 T5171 T5168 prep for,transferred
R4299 T5172 T5173 nummod 1,hour
R4300 T5173 T5171 pobj hour,for
R4301 T5174 T5173 prep on,hour
R4302 T5175 T5174 pobj ice,on
R4303 T5176 T5168 prep to,transferred
R4304 T5177 T5181 det a,membrane
R4305 T5178 T5179 advmod positively,charged
R4306 T5179 T5181 amod charged,membrane
R4307 T5180 T5181 compound nylon,membrane
R4308 T5181 T5176 pobj membrane,to
R4309 T5182 T5181 cc and,membrane
R4310 T5183 T5184 advmod immediately,cross-linked
R4311 T5184 T5181 conj cross-linked,membrane
R4312 T5185 T5184 prep for,cross-linked
R4313 T5186 T5187 nummod 15,minutes
R4314 T5187 T5185 pobj minutes,for
R4315 T5188 T5187 prep with,minutes
R4316 T5189 T5191 det a,transilluminator
R4317 T5190 T5191 compound UV,transilluminator
R4318 T5191 T5188 pobj transilluminator,with
R4319 T5192 T5187 acl equipped,minutes
R4320 T5193 T5192 prep with,equipped
R4321 T5194 T5197 det a,bulb
R4322 T5195 T5197 nummod 312,bulb
R4323 T5196 T5197 compound nm,bulb
R4324 T5197 T5193 pobj bulb,with
R4325 T5198 T5168 punct .,transferred
R4326 T5199 T5201 amod Streptavidin-horseradish,conjugate
R4327 T5200 T5201 compound peroxidase,conjugate
R4328 T5201 T5208 nsubjpass conjugate,used
R4329 T5202 T5201 cc and,conjugate
R4330 T5203 T5206 det the,Substrate
R4331 T5204 T5206 compound LightShift,Substrate
R4332 T5205 T5206 compound Chemiluminescent,Substrate
R4333 T5206 T5201 conj Substrate,conjugate
R4334 T5207 T5208 auxpass were,used
R4335 T5208 T5208 ROOT used,used
R4336 T5209 T5210 aux to,detect
R4337 T5210 T5208 xcomp detect,used
R4338 T5211 T5212 det the,biotin
R4339 T5212 T5210 dobj biotin,detect
R4340 T5213 T5214 compound end-labeled,DNA
R4341 T5214 T5208 npadvmod DNA,used
R4342 T5215 T5208 punct .,used
R4343 T5216 T5218 det The,membranes
R4344 T5217 T5218 compound nylon,membranes
R4345 T5218 T5220 nsubjpass membranes,exposed
R4346 T5219 T5220 auxpass were,exposed
R4347 T5220 T5220 ROOT exposed,exposed
R4348 T5221 T5222 aux to,x-ray
R4349 T5222 T5220 advcl x-ray,exposed
R4350 T5223 T5222 dobj film,x-ray
R4351 T5224 T5222 prep for,x-ray
R4352 T5225 T5226 quantmod 1,3
R4353 T5226 T5227 nummod 3,minutes
R4354 T5227 T5224 pobj minutes,for
R4355 T5228 T5227 prep for,minutes
R4356 T5229 T5228 pobj detection,for
R4357 T5230 T5220 punct .,exposed
R4682 T5650 T5694 nsubjpass Role,utilized
R4683 T5651 T5650 prep of,Role
R4684 T5652 T5651 pobj PKA,of
R4685 T5653 T5650 prep on,Role
R4686 T5654 T5656 amod LPS-induced,cell
R4687 T5655 T5656 compound mononuclear,cell
R4688 T5656 T5653 pobj cell,on
R4689 T5657 T5658 amod TNF-α,production
R4690 T5658 T5660 dative production,evaluate
R4691 T5659 T5660 aux To,evaluate
R4692 T5660 T5650 advcl evaluate,Role
R4693 T5661 T5662 det the,role
R4694 T5662 T5660 dobj role,evaluate
R4695 T5663 T5662 prep of,role
R4696 T5664 T5663 pobj PKA,of
R4697 T5665 T5662 prep on,role
R4698 T5666 T5667 amod TNF-α,production
R4699 T5667 T5665 pobj production,on
R4700 T5668 T5667 prep in,production
R4701 T5669 T5670 amod mononuclear,cells
R4702 T5670 T5668 pobj cells,in
R4703 T5671 T5670 cc and,cells
R4704 T5672 T5673 poss its,involvement
R4705 T5673 T5662 conj involvement,role
R4706 T5674 T5673 prep in,involvement
R4707 T5675 T5676 det the,attenuation
R4708 T5676 T5674 pobj attenuation,in
R4709 T5677 T5676 prep of,attenuation
R4710 T5678 T5680 amod LPS-induced,production
R4711 T5679 T5680 compound TNF-α,production
R4712 T5680 T5677 pobj production,of
R4713 T5681 T5694 ccomp observed,utilized
R4714 T5682 T5681 prep following,observed
R4715 T5683 T5684 compound PTX,treatment
R4716 T5684 T5682 pobj treatment,following
R4717 T5685 T5684 punct ",",treatment
R4718 T5686 T5694 nsubjpass H89,utilized
R4719 T5687 T5686 punct ",",H89
R4720 T5688 T5691 det a,inhibitor
R4721 T5689 T5691 amod specific,inhibitor
R4722 T5690 T5691 compound PKA,inhibitor
R4723 T5691 T5686 appos inhibitor,H89
R4724 T5692 T5694 punct ",",utilized
R4725 T5693 T5694 auxpass was,utilized
R4726 T5694 T5694 ROOT utilized,utilized
R4727 T5695 T5694 punct .,utilized
R4728 T5696 T5698 amod Isolated,cells
R4729 T5697 T5698 compound mononuclear,cells
R4730 T5698 T5700 nsubjpass cells,incubated
R4731 T5699 T5700 auxpass were,incubated
R4732 T5700 T5700 ROOT incubated,incubated
R4733 T5701 T5700 prep according,incubated
R4734 T5702 T5701 prep to,according
R4735 T5703 T5705 det the,groups
R4736 T5704 T5705 compound treatment,groups
R4737 T5705 T5702 pobj groups,to
R4738 T5706 T5705 acl described,groups
R4739 T5707 T5706 advmod above,described
R4740 T5708 T5706 prep in,described
R4741 T5709 T5710 det the,presence
R4742 T5710 T5708 pobj presence,in
R4743 T5711 T5710 cc and,presence
R4744 T5712 T5710 conj absence,presence
R4745 T5713 T5712 prep of,absence
R4746 T5714 T5713 pobj pretreatment,of
R4747 T5715 T5706 prep with,described
R4748 T5716 T5715 pobj H89,with
R4749 T5717 T5719 punct (,μM
R4750 T5718 T5719 nummod 10,μM
R4751 T5719 T5716 appos μM,H89
R4752 T5720 T5701 punct ),according
R4753 T5721 T5700 prep for,incubated
R4754 T5722 T5723 nummod 1,hour
R4755 T5723 T5721 pobj hour,for
R4756 T5724 T5700 prep at,incubated
R4757 T5725 T5724 pobj 37ºC,at
R4758 T5726 T5700 punct .,incubated
R4759 T5727 T5728 det The,dose
R4760 T5728 T5732 nsubjpass dose,chosen
R4761 T5729 T5728 prep of,dose
R4762 T5730 T5729 pobj H89,of
R4763 T5731 T5732 auxpass was,chosen
R4764 T5732 T5732 ROOT chosen,chosen
R4765 T5733 T5732 prep based,chosen
R4766 T5734 T5733 prep on,based
R4767 T5735 T5736 amod previous,work
R4768 T5736 T5734 pobj work,on
R4769 T5737 T5738 nsubj which,demonstrated
R4770 T5738 T5736 relcl demonstrated,work
R4771 T5739 T5742 amod specific,inhibition
R4772 T5740 T5739 cc and,specific
R4773 T5741 T5739 conj complete,specific
R4774 T5742 T5738 dobj inhibition,demonstrated
R4775 T5743 T5742 prep of,inhibition
R4776 T5744 T5743 pobj PKA,of
R4777 T5745 T5738 prep at,demonstrated
R4778 T5746 T5747 det this,concentration
R4779 T5747 T5745 pobj concentration,at
R4780 T5748 T5747 nummod .10,concentration
R4781 T5749 T5755 mark Since,is
R4782 T5750 T5751 det the,activity
R4783 T5751 T5749 pobj activity,Since
R4784 T5752 T5751 prep of,activity
R4785 T5753 T5754 det this,inhibitor
R4786 T5754 T5752 pobj inhibitor,of
R4787 T5755 T5768 advcl is,include
R4788 T5756 T5757 preconj both,specific
R4789 T5757 T5755 acomp specific,is
R4790 T5758 T5757 cc and,specific
R4791 T5759 T5757 conj consistent,specific
R4792 T5760 T5759 prep at,consistent
R4793 T5761 T5763 det this,dose
R4794 T5762 T5763 amod specified,dose
R4795 T5763 T5760 pobj dose,at
R4796 T5764 T5768 punct ",",include
R4797 T5765 T5768 nsubj we,include
R4798 T5766 T5768 aux did,include
R4799 T5767 T5768 neg not,include
R4800 T5768 T5732 advcl include,chosen
R4801 T5769 T5768 dobj experiments,include
R4802 T5770 T5769 prep with,experiments
R4803 T5771 T5773 amod additional,inhibitors
R4804 T5772 T5773 compound PKA,inhibitors
R4805 T5773 T5770 pobj inhibitors,with
R4806 T5774 T5769 prep with,experiments
R4807 T5775 T5776 det this,set
R4808 T5776 T5774 pobj set,with
R4809 T5777 T5776 prep of,set
R4810 T5778 T5777 pobj experiments,of
R4811 T5779 T5768 punct .,include
R4812 T5780 T5782 det The,concentration
R4813 T5781 T5782 amod TNF-α,concentration
R4814 T5782 T5787 nsubjpass concentration,measured
R4815 T5783 T5782 prep in,concentration
R4816 T5784 T5785 det the,supernatant
R4817 T5785 T5783 pobj supernatant,in
R4818 T5786 T5787 auxpass was,measured
R4819 T5787 T5787 ROOT measured,measured
R4820 T5788 T5787 advmod quantitatively,measured
R4821 T5789 T5787 agent by,measured
R4822 T5790 T5789 pobj ELISA,by
R4823 T5791 T5787 punct .,measured
R4824 T5792 T5793 det The,results
R4825 T5793 T5795 nsubjpass results,expressed
R4826 T5794 T5795 auxpass are,expressed
R4827 T5795 T5795 ROOT expressed,expressed
R4828 T5796 T5795 prep in,expressed
R4829 T5797 T5796 pobj pg/mL,in
R4830 T5798 T5795 punct .,expressed
R5035 T6072 T6073 compound Statistical,Analysis
R5036 T6073 T6082 nsubjpass Analysis,derived
R5037 T6074 T6076 det The,results
R5038 T6075 T6076 amod experimental,results
R5039 T6076 T6082 nsubjpass results,derived
R5040 T6077 T6076 acl obtained,results
R5041 T6078 T6077 prep in,obtained
R5042 T6079 T6080 det this,study
R5043 T6080 T6078 pobj study,in
R5044 T6081 T6082 auxpass were,derived
R5045 T6082 T6082 ROOT derived,derived
R5046 T6083 T6082 prep from,derived
R5047 T6084 T6086 nummod four,experiments
R5048 T6085 T6086 amod separate,experiments
R5049 T6086 T6083 pobj experiments,from
R5050 T6087 T6086 prep with,experiments
R5051 T6088 T6090 amod healthy,donors
R5052 T6089 T6090 compound volunteer,donors
R5053 T6090 T6087 pobj donors,with
R5054 T6091 T6082 punct .,derived
R5055 T6092 T6093 det Each,assay
R5056 T6093 T6095 nsubjpass assay,performed
R5057 T6094 T6095 auxpass was,performed
R5058 T6095 T6095 ROOT performed,performed
R5059 T6096 T6095 prep in,performed
R5060 T6097 T6096 pobj duplicate,in
R5061 T6098 T6097 cc or,duplicate
R5062 T6099 T6097 conj triplicate,duplicate
R5063 T6100 T6101 advmod where,appropriate
R5064 T6101 T6099 advcl appropriate,triplicate
R5065 T6102 T6095 punct .,performed
R5066 T6103 T6105 nsubjpass Data,presented
R5067 T6104 T6105 auxpass is,presented
R5068 T6105 T6105 ROOT presented,presented
R5069 T6106 T6105 prep as,presented
R5070 T6107 T6110 det the,SEM
R5071 T6108 T6110 amod mean,SEM
R5072 T6109 T6110 compound ±,SEM
R5073 T6110 T6106 pobj SEM,as
R5074 T6111 T6105 punct .,presented
R5075 T6112 T6113 compound Statistical,differences
R5076 T6113 T6117 nsubjpass differences,determined
R5077 T6114 T6113 prep between,differences
R5078 T6115 T6114 pobj groups,between
R5079 T6116 T6117 auxpass were,determined
R5080 T6117 T6117 ROOT determined,determined
R5081 T6118 T6117 agent by,determined
R5082 T6119 T6120 amod one-way,analysis
R5083 T6120 T6118 pobj analysis,by
R5084 T6121 T6120 prep of,analysis
R5085 T6122 T6121 pobj variance,of
R5086 T6123 T6124 punct (,ANOVA
R5087 T6124 T6122 appos ANOVA,variance
R5088 T6125 T6124 punct ),ANOVA
R5089 T6126 T6120 prep with,analysis
R5090 T6127 T6129 det a,correction
R5091 T6128 T6129 compound Bonferroni,correction
R5092 T6129 T6126 pobj correction,with
R5093 T6130 T6117 punct .,determined
R5094 T6131 T6132 compound Statistical,significance
R5095 T6132 T6134 nsubjpass significance,defined
R5096 T6133 T6134 auxpass was,defined
R5097 T6134 T6134 ROOT defined,defined
R5098 T6135 T6134 prep as,defined
R5099 T6136 T6135 pobj P,as
R5100 T6137 T6136 nummod <,P
R5101 T6138 T6136 nummod 0.05,P
R5102 T6139 T6134 punct .,defined
R5186 T6242 T6243 det The,effects
R5187 T6243 T6251 nsubj effects,determine
R5188 T6244 T6243 prep of,effects
R5189 T6245 T6244 pobj PTX,of
R5190 T6246 T6243 prep on,effects
R5191 T6247 T6249 amod LPS-induced,production
R5192 T6248 T6249 amod TNF-α,production
R5193 T6249 T6246 pobj production,on
R5194 T6250 T6251 aux To,determine
R5195 T6251 T6251 ROOT determine,determine
R5196 T6252 T6253 det the,duration
R5197 T6253 T6251 dobj duration,determine
R5198 T6254 T6253 prep of,duration
R5199 T6255 T6256 compound PDE,inhibition
R5200 T6256 T6254 pobj inhibition,of
R5201 T6257 T6256 prep on,inhibition
R5202 T6258 T6259 amod TNF-α,production
R5203 T6259 T6257 pobj production,on
R5204 T6260 T6251 prep after,determine
R5205 T6261 T6262 compound LPS,stimulation
R5206 T6262 T6260 pobj stimulation,after
R5207 T6263 T6268 punct ",",conducted
R5208 T6264 T6268 npadvmod time,conducted
R5209 T6265 T6266 compound course,studies
R5210 T6266 T6268 nsubjpass studies,conducted
R5211 T6267 T6268 auxpass were,conducted
R5212 T6268 T6251 advcl conducted,determine
R5213 T6269 T6251 punct .,determine
R5214 T6270 T6277 prep In,had
R5215 T6271 T6270 pobj accordance,In
R5216 T6272 T6271 prep with,accordance
R5217 T6273 T6274 amod prior,work
R5218 T6274 T6272 pobj work,with
R5219 T6275 T6277 punct ",",had
R5220 T6276 T6277 nsubj PTX,had
R5221 T6277 T6277 ROOT had,had
R5222 T6278 T6282 det a,effect
R5223 T6279 T6282 amod rapid,effect
R5224 T6280 T6279 cc and,rapid
R5225 T6281 T6279 conj sustained,rapid
R5226 T6282 T6277 dobj effect,had
R5227 T6283 T6282 prep on,effect
R5228 T6284 T6285 amod TNF-α,production
R5229 T6285 T6283 pobj production,on
R5230 T6286 T6285 prep in,production
R5231 T6287 T6289 amod vitro,Figure
R5232 T6288 T6289 punct (,Figure
R5233 T6289 T6286 pobj Figure,in
R5234 T6290 T6289 nummod 1,Figure
R5235 T6291 T6289 punct ),Figure
R5236 T6292 T6277 punct .,had
R5380 T6505 T6506 nsubj PTX,decreases
R5381 T6506 T6506 ROOT decreases,decreases
R5382 T6507 T6509 amod LPS-induced,activation
R5383 T6508 T6509 compound NF-κB,activation
R5384 T6509 T6506 dobj activation,decreases
R5385 T6510 T6512 mark Since,leads
R5386 T6511 T6512 nsubj phosphorylation,leads
R5387 T6512 T6528 advcl leads,examined
R5388 T6513 T6512 prep to,leads
R5389 T6514 T6513 pobj ubiquitination,to
R5390 T6515 T6514 cc and,ubiquitination
R5391 T6516 T6514 conj degradation,ubiquitination
R5392 T6517 T6516 prep of,degradation
R5393 T6518 T6517 pobj I-κBα,of
R5394 T6519 T6518 cc and,I-κBα
R5395 T6520 T6522 amod subsequent,translocation
R5396 T6521 T6522 amod nuclear,translocation
R5397 T6522 T6518 conj translocation,I-κBα
R5398 T6523 T6522 prep of,translocation
R5399 T6524 T6523 pobj NF-κB,of
R5400 T6525 T6528 punct ",",examined
R5401 T6526 T6528 nsubj we,examined
R5402 T6527 T6528 advmod first,examined
R5403 T6528 T6506 advcl examined,decreases
R5404 T6529 T6530 det the,effects
R5405 T6530 T6528 dobj effects,examined
R5406 T6531 T6530 prep of,effects
R5407 T6532 T6531 pobj PTX,of
R5408 T6533 T6530 prep on,effects
R5409 T6534 T6533 pobj I-κBα,on
R5410 T6535 T6506 punct .,decreases
R5411 T6536 T6538 compound Cytoplasmic,phosphorylation
R5412 T6537 T6538 compound I-κBα,phosphorylation
R5413 T6538 T6541 nsubjpass phosphorylation,increased
R5414 T6539 T6541 auxpass was,increased
R5415 T6540 T6541 advmod markedly,increased
R5416 T6541 T6541 ROOT increased,increased
R5417 T6542 T6541 prep following,increased
R5418 T6543 T6544 compound LPS,stimulation
R5419 T6544 T6542 pobj stimulation,following
R5420 T6545 T6546 advmod when,compared
R5421 T6546 T6541 advcl compared,increased
R5422 T6547 T6548 aux to,control
R5423 T6548 T6546 xcomp control,compared
R5424 T6549 T6552 punct (,0
R5425 T6550 T6552 nummod 100,0
R5426 T6551 T6552 nummod ±,0
R5427 T6552 T6548 dobj 0,control
R5428 T6553 T6552 prep vs.,0
R5429 T6554 T6556 nummod 20,18
R5430 T6555 T6556 nummod ±,18
R5431 T6556 T6553 pobj 18,vs.
R5432 T6557 T6560 punct ;,0.05
R5433 T6558 T6559 compound P,<
R5434 T6559 T6560 compound <,0.05
R5435 T6560 T6541 npadvmod 0.05,increased
R5436 T6561 T6560 punct ),0.05
R5437 T6562 T6541 punct .,increased
R5438 T6563 T6564 det The,addition
R5439 T6564 T6568 nsubj addition,downregulated
R5440 T6565 T6564 prep of,addition
R5441 T6566 T6565 pobj PTX,of
R5442 T6567 T6568 advmod significantly,downregulated
R5443 T6568 T6568 ROOT downregulated,downregulated
R5444 T6569 T6571 amod LPS-induced,phosphorylation
R5445 T6570 T6571 amod I-κBα,phosphorylation
R5446 T6571 T6568 dobj phosphorylation,downregulated
R5447 T6572 T6573 punct (,P
R5448 T6573 T6571 appos P,phosphorylation
R5449 T6574 T6573 punct =,P
R5450 T6575 T6573 nummod 0.02,P
R5451 T6576 T6573 punct ;,P
R5452 T6577 T6577 ROOT Figure,Figure
R5453 T6578 T6577 nummod 2,Figure
R5454 T6579 T6577 punct ),Figure
R5455 T6580 T6590 prep In,increased
R5456 T6581 T6583 det a,fashion
R5457 T6582 T6583 amod similar,fashion
R5458 T6583 T6580 pobj fashion,In
R5459 T6584 T6590 punct ",",increased
R5460 T6585 T6588 amod nuclear,phosphorylation
R5461 T6586 T6588 nmod NF-κB,phosphorylation
R5462 T6587 T6588 nummod p65,phosphorylation
R5463 T6588 T6590 nsubjpass phosphorylation,increased
R5464 T6589 T6590 auxpass was,increased
R5465 T6590 T6590 ROOT increased,increased
R5466 T6591 T6590 prep following,increased
R5467 T6592 T6593 compound LPS,stimulation
R5468 T6593 T6591 pobj stimulation,following
R5469 T6594 T6597 punct (,0
R5470 T6595 T6597 nummod 100,0
R5471 T6596 T6597 nummod ±,0
R5472 T6597 T6590 npadvmod 0,increased
R5473 T6598 T6597 prep vs.,0
R5474 T6599 T6601 nummod 38,20
R5475 T6600 T6601 nummod ±,20
R5476 T6601 T6598 pobj 20,vs.
R5477 T6602 T6605 punct ;,0.01
R5478 T6603 T6604 compound P,<
R5479 T6604 T6605 compound <,0.01
R5480 T6605 T6590 npadvmod 0.01,increased
R5481 T6606 T6605 punct ),0.01
R5482 T6607 T6590 punct .,increased
R5483 T6608 T6610 nsubj PTX,decreased
R5484 T6609 T6610 advmod similarly,decreased
R5485 T6610 T6610 ROOT decreased,decreased
R5486 T6611 T6615 amod LPS-induced,phosphorylation
R5487 T6612 T6615 nmod NF-κB,phosphorylation
R5488 T6613 T6612 nummod p65,NF-κB
R5489 T6614 T6615 amod nuclear,phosphorylation
R5490 T6615 T6610 dobj phosphorylation,decreased
R5491 T6616 T6615 punct ",",phosphorylation
R5492 T6617 T6618 det a,marker
R5493 T6618 T6615 appos marker,phosphorylation
R5494 T6619 T6618 prep of,marker
R5495 T6620 T6621 amod NF-κB,activation
R5496 T6621 T6619 pobj activation,of
R5497 T6622 T6621 cc and,activation
R5498 T6623 T6624 amod nuclear,translocation
R5499 T6624 T6621 conj translocation,activation
R5500 T6625 T6628 punct (,0
R5501 T6626 T6628 nummod 100,0
R5502 T6627 T6628 nummod ±,0
R5503 T6628 T6615 appos 0,phosphorylation
R5504 T6629 T6630 quantmod vs.,40
R5505 T6630 T6631 nummod 40,±
R5506 T6631 T6628 pobj ±,0
R5507 T6632 T6631 nummod 6,±
R5508 T6633 T6631 punct ;,±
R5509 T6634 T6636 compound P,0.03
R5510 T6635 T6636 punct =,0.03
R5511 T6636 T6631 conj 0.03,±
R5512 T6637 T6636 punct ;,0.03
R5513 T6638 T6639 compound Figure,3A
R5514 T6639 T6636 appos 3A,0.03
R5515 T6640 T6636 punct ),0.03
R5516 T6641 T6631 punct .,±
R5517 T6642 T6645 nsubjpass EMSAs,performed
R5518 T6643 T6645 auxpass were,performed
R5519 T6644 T6645 advmod then,performed
R5520 T6645 T6645 ROOT performed,performed
R5521 T6646 T6647 aux to,verify
R5522 T6647 T6645 advcl verify,performed
R5523 T6648 T6650 det the,alterations
R5524 T6649 T6650 amod PTX-induced,alterations
R5525 T6650 T6647 dobj alterations,verify
R5526 T6651 T6650 prep in,alterations
R5527 T6652 T6653 amod nuclear,phosphorylation
R5528 T6653 T6651 pobj phosphorylation,in
R5529 T6654 T6645 conj resulted,performed
R5530 T6655 T6654 prep in,resulted
R5531 T6656 T6657 amod similar,effects
R5532 T6657 T6655 pobj effects,in
R5533 T6658 T6657 prep on,effects
R5534 T6659 T6662 det the,activity
R5535 T6660 T6662 compound DNA,activity
R5536 T6661 T6662 amod binding,activity
R5537 T6662 T6658 pobj activity,on
R5538 T6663 T6662 prep of,activity
R5539 T6664 T6663 pobj NF-κB,of
R5540 T6665 T6645 punct .,performed
R5541 T6666 T6667 det The,addition
R5542 T6667 T6674 nsubj addition,resulted
R5543 T6668 T6667 prep of,addition
R5544 T6669 T6668 pobj PTX,of
R5545 T6670 T6667 prep to,addition
R5546 T6671 T6673 amod LPS-stimulated,cells
R5547 T6672 T6673 compound mononuclear,cells
R5548 T6673 T6670 pobj cells,to
R5549 T6674 T6674 ROOT resulted,resulted
R5550 T6675 T6674 prep in,resulted
R5551 T6676 T6677 amod comparable,downregulation
R5552 T6677 T6675 pobj downregulation,in
R5553 T6678 T6677 prep of,downregulation
R5554 T6679 T6678 pobj DNA,of
R5555 T6680 T6681 amod binding,activity
R5556 T6681 T6677 conj activity,downregulation
R5557 T6682 T6681 punct ",",activity
R5558 T6683 T6681 amod similar,activity
R5559 T6684 T6683 prep to,similar
R5560 T6685 T6687 det the,downregulation
R5561 T6686 T6687 amod observed,downregulation
R5562 T6687 T6684 pobj downregulation,to
R5563 T6688 T6687 prep of,downregulation
R5564 T6689 T6690 amod NF-κB,phosphorylation
R5565 T6690 T6688 pobj phosphorylation,of
R5566 T6691 T6693 punct (,3B
R5567 T6692 T6693 compound Figure,3B
R5568 T6693 T6690 appos 3B,phosphorylation
R5569 T6694 T6690 punct ),phosphorylation
R5570 T6695 T6674 punct .,resulted
R5876 T7091 T7092 nsubj PTX,upregulates
R5877 T7092 T7092 ROOT upregulates,upregulates
R5878 T7093 T7094 compound CREB,phosphorylation
R5879 T7094 T7092 dobj phosphorylation,upregulates
R5880 T7095 T7094 cc and,phosphorylation
R5881 T7096 T7094 conj activation,phosphorylation
R5882 T7097 T7105 mark after,used
R5883 T7098 T7100 compound LPS,Phosphorylation
R5884 T7099 T7100 compound stimulation,Phosphorylation
R5885 T7100 T7105 nsubjpass Phosphorylation,used
R5886 T7101 T7100 prep of,Phosphorylation
R5887 T7102 T7103 amod nuclear,CREB
R5888 T7103 T7101 pobj CREB,of
R5889 T7104 T7105 auxpass was,used
R5890 T7105 T7092 advcl used,upregulates
R5891 T7106 T7105 prep as,used
R5892 T7107 T7108 det a,marker
R5893 T7108 T7106 pobj marker,as
R5894 T7109 T7108 prep for,marker
R5895 T7110 T7111 compound CREB,activation
R5896 T7111 T7109 pobj activation,for
R5897 T7112 T7092 punct .,upregulates
R5898 T7113 T7114 compound LPS,stimulation
R5899 T7114 T7115 nsubj stimulation,caused
R5900 T7115 T7115 ROOT caused,caused
R5901 T7116 T7118 det a,increase
R5902 T7117 T7118 amod negligible,increase
R5903 T7118 T7115 dobj increase,caused
R5904 T7119 T7118 prep in,increase
R5905 T7120 T7121 compound CREB,phosphorylation
R5906 T7121 T7119 pobj phosphorylation,in
R5907 T7122 T7123 advmod when,compared
R5908 T7123 T7115 advcl compared,caused
R5909 T7124 T7125 aux to,control
R5910 T7125 T7123 xcomp control,compared
R5911 T7126 T7128 punct (,4A
R5912 T7127 T7128 compound Figure,4A
R5913 T7128 T7125 dobj 4A,control
R5914 T7129 T7128 punct ),4A
R5915 T7130 T7115 punct .,caused
R5916 T7131 T7133 nsubj PTX,caused
R5917 T7132 T7131 advmod alone,PTX
R5918 T7133 T7133 ROOT caused,caused
R5919 T7134 T7136 det a,increase
R5920 T7135 T7136 amod marked,increase
R5921 T7136 T7133 dobj increase,caused
R5922 T7137 T7136 prep in,increase
R5923 T7138 T7139 compound CREB,phosphorylation
R5924 T7139 T7137 pobj phosphorylation,in
R5925 T7140 T7141 punct (,P
R5926 T7141 T7136 appos P,increase
R5927 T7142 T7143 compound <,0.01
R5928 T7143 T7141 nummod 0.01,P
R5929 T7144 T7141 prep vs.,P
R5930 T7145 T7144 pobj HBSS,vs.
R5931 T7146 T7141 punct ),P
R5932 T7147 T7133 punct .,caused
R5933 T7148 T7153 advmod When,occurred
R5934 T7149 T7153 nsubj LPS,occurred
R5935 T7150 T7149 cc and,LPS
R5936 T7151 T7149 conj PTX,LPS
R5937 T7152 T7149 conj exposure,LPS
R5938 T7153 T7158 advcl occurred,was
R5939 T7154 T7153 advmod simultaneously,occurred
R5940 T7155 T7158 punct ",",was
R5941 T7156 T7157 compound CREB,phosphorylation
R5942 T7157 T7158 nsubj phosphorylation,was
R5943 T7158 T7158 ROOT was,was
R5944 T7159 T7160 advmod significantly,higher
R5945 T7160 T7158 acomp higher,was
R5946 T7161 T7160 prep than,higher
R5947 T7162 T7161 prep with,than
R5948 T7163 T7164 compound LPS,stimulation
R5949 T7164 T7162 pobj stimulation,with
R5950 T7165 T7164 advmod alone,stimulation
R5951 T7166 T7164 punct (,stimulation
R5952 T7167 T7169 nummod 543,92
R5953 T7168 T7169 nummod ±,92
R5954 T7169 T7164 appos 92,stimulation
R5955 T7170 T7169 prep vs.,92
R5956 T7171 T7173 nummod 100,0
R5957 T7172 T7173 nummod ±,0
R5958 T7173 T7170 pobj 0,vs.
R5959 T7174 T7176 punct ;,<
R5960 T7175 T7176 compound P,<
R5961 T7176 T7158 attr <,was
R5962 T7177 T7176 nummod 0.01,<
R5963 T7178 T7176 punct ),<
R5964 T7179 T7158 punct .,was
R5965 T7180 T7195 prep In,was
R5966 T7181 T7180 pobj addition,In
R5967 T7182 T7195 punct ",",was
R5968 T7183 T7184 det the,amount
R5969 T7184 T7195 nsubj amount,was
R5970 T7185 T7184 prep of,amount
R5971 T7186 T7187 compound CREB,phosphorylation
R5972 T7187 T7185 pobj phosphorylation,of
R5973 T7188 T7184 acl seen,amount
R5974 T7189 T7188 prep with,seen
R5975 T7190 T7191 amod concomitant,LPS
R5976 T7191 T7189 pobj LPS,with
R5977 T7192 T7191 cc and,LPS
R5978 T7193 T7194 compound PTX,treatment
R5979 T7194 T7184 conj treatment,amount
R5980 T7195 T7195 ROOT was,was
R5981 T7196 T7195 acomp less,was
R5982 T7197 T7196 prep than,less
R5983 T7198 T7197 pobj that,than
R5984 T7199 T7198 acl seen,that
R5985 T7200 T7199 prep with,seen
R5986 T7201 T7200 pobj PTX,with
R5987 T7202 T7201 advmod alone,PTX
R5988 T7203 T7195 punct ",",was
R5989 T7204 T7207 mark although,was
R5990 T7205 T7206 det this,difference
R5991 T7206 T7207 nsubj difference,was
R5992 T7207 T7195 advcl was,was
R5993 T7208 T7207 neg not,was
R5994 T7209 T7210 advmod statistically,significant
R5995 T7210 T7207 acomp significant,was
R5996 T7211 T7214 punct (,0.08
R5997 T7212 T7214 dep P,0.08
R5998 T7213 T7214 punct =,0.08
R5999 T7214 T7210 parataxis 0.08,significant
R6000 T7215 T7214 punct ),0.08
R6001 T7216 T7195 punct .,was
R6002 T7217 T7218 aux To,determine
R6003 T7218 T7237 advcl determine,performed
R6004 T7219 T7223 mark if,affected
R6005 T7220 T7221 compound CREB-DNA,binding
R6006 T7221 T7223 nsubjpass binding,affected
R6007 T7222 T7223 auxpass was,affected
R6008 T7223 T7237 advcl affected,performed
R6009 T7224 T7223 agent by,affected
R6010 T7225 T7224 pobj PTX,by
R6011 T7226 T7223 prep in,affected
R6012 T7227 T7228 det a,manner
R6013 T7228 T7226 pobj manner,in
R6014 T7229 T7228 amod similar,manner
R6015 T7230 T7229 prep to,similar
R6016 T7231 T7232 compound CREB,phosphorylation
R6017 T7232 T7230 pobj phosphorylation,to
R6018 T7233 T7237 punct ",",performed
R6019 T7234 T7235 det an,EMSA
R6020 T7235 T7237 nsubjpass EMSA,performed
R6021 T7236 T7237 auxpass was,performed
R6022 T7237 T7237 ROOT performed,performed
R6023 T7238 T7237 punct .,performed
R6024 T7239 T7246 nsubj Exposure,increased
R6025 T7240 T7239 prep of,Exposure
R6026 T7241 T7242 amod LPS-stimulated,cells
R6027 T7242 T7240 pobj cells,of
R6028 T7243 T7239 prep to,Exposure
R6029 T7244 T7243 pobj PTX,to
R6030 T7245 T7246 advmod similarly,increased
R6031 T7246 T7246 ROOT increased,increased
R6032 T7247 T7248 compound CREB,DNA-binding
R6033 T7248 T7246 dobj DNA-binding,increased
R6034 T7249 T7246 prep compared,increased
R6035 T7250 T7249 prep to,compared
R6036 T7251 T7250 pobj LPS,to
R6037 T7252 T7251 advmod alone,LPS
R6038 T7253 T7255 punct (,4B
R6039 T7254 T7255 compound Figure,4B
R6040 T7255 T7251 appos 4B,LPS
R6041 T7256 T7246 punct ),increased
R6042 T7257 T7246 punct .,increased
R6273 T7611 T7611 ROOT Effect,Effect
R6274 T7612 T7611 prep of,Effect
R6275 T7613 T7612 pobj PTX,of
R6276 T7614 T7611 prep on,Effect
R6277 T7615 T7616 amod TNF-α,production
R6278 T7616 T7614 pobj production,on
R6279 T7617 T7616 prep in,production
R6280 T7618 T7619 det the,presence
R6281 T7619 T7617 pobj presence,in
R6282 T7620 T7619 prep of,presence
R6283 T7621 T7622 compound PKA,inhibition
R6284 T7622 T7620 pobj inhibition,of
R6285 T7623 T7624 det The,role
R6286 T7624 T7638 nsubjpass role,assessed
R6287 T7625 T7624 prep of,role
R6288 T7626 T7625 pobj PKA,of
R6289 T7627 T7624 prep in,role
R6290 T7628 T7629 amod PTX-induced,downregulation
R6291 T7629 T7627 pobj downregulation,in
R6292 T7630 T7629 prep of,downregulation
R6293 T7631 T7632 amod TNF-α,production
R6294 T7632 T7630 pobj production,of
R6295 T7633 T7632 prep in,production
R6296 T7634 T7636 amod LPS-stimulated,cells
R6297 T7635 T7636 compound mononuclear,cells
R6298 T7636 T7633 pobj cells,in
R6299 T7637 T7638 auxpass was,assessed
R6300 T7638 T7638 ROOT assessed,assessed
R6301 T7639 T7638 prep by,assessed
R6302 T7640 T7639 pcomp treating,by
R6303 T7641 T7642 compound mononuclear,cells
R6304 T7642 T7640 dobj cells,treating
R6305 T7643 T7640 prep with,treating
R6306 T7644 T7643 pobj H89,with
R6307 T7645 T7644 punct ",",H89
R6308 T7646 T7648 det a,inhibitor
R6309 T7647 T7648 compound PKA,inhibitor
R6310 T7648 T7644 appos inhibitor,H89
R6311 T7649 T7644 punct ",",H89
R6312 T7650 T7640 advmod prior,treating
R6313 T7651 T7650 prep to,prior
R6314 T7652 T7651 pobj LPS,to
R6315 T7653 T7652 cc and,LPS
R6316 T7654 T7655 compound PTX,exposure
R6317 T7655 T7652 conj exposure,LPS
R6318 T7656 T7638 punct .,assessed
R6319 T7657 T7658 det The,addition
R6320 T7658 T7659 nsubj addition,PTX
R6321 T7659 T7663 nsubj PTX,resulted
R6322 T7660 T7659 prep to,PTX
R6323 T7661 T7662 amod LPS-stimulated,cells
R6324 T7662 T7660 pobj cells,to
R6325 T7663 T7663 ROOT resulted,resulted
R6326 T7664 T7663 prep in,resulted
R6327 T7665 T7666 det a,reduction
R6328 T7666 T7664 pobj reduction,in
R6329 T7667 T7666 prep in,reduction
R6330 T7668 T7667 pobj TNF-α,in
R6331 T7669 T7668 punct ",",TNF-α
R6332 T7670 T7666 amod similar,reduction
R6333 T7671 T7673 aux to,observed
R6334 T7672 T7673 nsubj that,observed
R6335 T7673 T7666 relcl observed,reduction
R6336 T7674 T7673 prep in,observed
R6337 T7675 T7677 poss our,experiments
R6338 T7676 T7677 amod previous,experiments
R6339 T7677 T7674 pobj experiments,in
R6340 T7678 T7677 punct (,experiments
R6341 T7679 T7684 quantmod 529,37
R6342 T7680 T7683 nmod pg/mL,vs.
R6343 T7681 T7683 nmod ±,vs.
R6344 T7682 T7681 nummod 112,±
R6345 T7683 T7684 nmod vs.,37
R6346 T7684 T7687 nummod 37,10
R6347 T7685 T7686 compound pg/mL,±
R6348 T7686 T7687 nummod ±,10
R6349 T7687 T7677 appos 10,experiments
R6350 T7688 T7677 punct ;,experiments
R6351 T7689 T7690 compound P,<
R6352 T7690 T7677 appos <,experiments
R6353 T7691 T7690 nummod 0.01,<
R6354 T7692 T7677 punct ),experiments
R6355 T7693 T7663 punct .,resulted
R6356 T7694 T7707 nsubj Pre-incubation,result
R6357 T7695 T7694 prep of,Pre-incubation
R6358 T7696 T7695 pobj cells,of
R6359 T7697 T7696 prep with,cells
R6360 T7698 T7697 pobj H89,with
R6361 T7699 T7707 advmod prior,result
R6362 T7700 T7699 prep to,prior
R6363 T7701 T7700 pobj LPS,to
R6364 T7702 T7701 cc and,LPS
R6365 T7703 T7704 compound PTX,exposure
R6366 T7704 T7701 conj exposure,LPS
R6367 T7705 T7707 aux did,result
R6368 T7706 T7707 neg not,result
R6369 T7707 T7707 ROOT result,result
R6370 T7708 T7707 prep in,result
R6371 T7709 T7711 det any,modulation
R6372 T7710 T7711 amod significant,modulation
R6373 T7711 T7708 pobj modulation,in
R6374 T7712 T7711 prep of,modulation
R6375 T7713 T7714 compound TNF-α,production
R6376 T7714 T7712 pobj production,of
R6377 T7715 T7716 advmod when,compared
R6378 T7716 T7707 advcl compared,result
R6379 T7717 T7716 prep to,compared
R6380 T7718 T7717 pobj that,to
R6381 T7719 T7718 acl demonstrated,that
R6382 T7720 T7719 prep with,demonstrated
R6383 T7721 T7720 pobj PTX,with
R6384 T7722 T7721 advmod alone,PTX
R6385 T7723 T7721 punct (,PTX
R6386 T7724 T7721 appos P,PTX
R6387 T7725 T7726 punct =,0.2
R6388 T7726 T7724 nummod 0.2,P
R6389 T7727 T7728 punct ;,Figure
R6390 T7728 T7724 appos Figure,P
R6391 T7729 T7728 nummod 5,Figure
R6392 T7730 T7728 punct ),Figure
R6393 T7731 T7707 punct .,result
R6394 T7732 T7733 det These,results
R6395 T7733 T7734 nsubj results,suggest
R6396 T7734 T7734 ROOT suggest,suggest
R6397 T7735 T7746 mark that,is
R6398 T7736 T7746 nsubj downregulation,is
R6399 T7737 T7736 prep of,downregulation
R6400 T7738 T7739 compound TNF-α,production
R6401 T7739 T7737 pobj production,of
R6402 T7740 T7739 prep by,production
R6403 T7741 T7740 pobj PTX,by
R6404 T7742 T7736 prep in,downregulation
R6405 T7743 T7745 amod LPS-stimulated,cells
R6406 T7744 T7745 compound mononuclear,cells
R6407 T7745 T7742 pobj cells,in
R6408 T7746 T7734 ccomp is,suggest
R6409 T7747 T7746 punct ",",is
R6410 T7748 T7749 advmod at,least
R6411 T7749 T7750 advmod least,in
R6412 T7750 T7746 prep in,is
R6413 T7751 T7750 pobj part,in
R6414 T7752 T7753 punct ",",PKA-independent
R6415 T7753 T7746 acomp PKA-independent,is
R6416 T7754 T7734 punct .,suggest
R6832 T8411 T8412 det The,exposure
R6833 T8412 T8422 nsubj exposure,results
R6834 T8413 T8412 prep of,exposure
R6835 T8414 T8415 amod inflammatory,cells
R6836 T8415 T8413 pobj cells,of
R6837 T8416 T8412 prep to,exposure
R6838 T8417 T8418 det a,variety
R6839 T8418 T8416 pobj variety,to
R6840 T8419 T8418 prep of,variety
R6841 T8420 T8421 amod extracellular,stimuli
R6842 T8421 T8419 pobj stimuli,of
R6843 T8422 T8444 dep results,released
R6844 T8423 T8422 prep in,results
R6845 T8424 T8425 det the,initiation
R6846 T8425 T8423 pobj initiation,in
R6847 T8426 T8425 prep of,initiation
R6848 T8427 T8430 amod transcriptional,events
R6849 T8428 T8427 cc and,transcriptional
R6850 T8429 T8427 conj posttranscriptional,transcriptional
R6851 T8430 T8426 pobj events,of
R6852 T8431 T8430 punct ",",events
R6853 T8432 T8433 nsubj which,culminate
R6854 T8433 T8430 relcl culminate,events
R6855 T8434 T8433 prep in,culminate
R6856 T8435 T8436 det the,production
R6857 T8436 T8434 pobj production,in
R6858 T8437 T8436 prep of,production
R6859 T8438 T8439 amod pro-inflammatory,mediators
R6860 T8439 T8437 pobj mediators,of
R6861 T8440 T8439 appos .11,mediators
R6862 T8441 T8442 nummod 14,TNF-α
R6863 T8442 T8444 nsubjpass TNF-α,released
R6864 T8443 T8444 auxpass is,released
R6865 T8444 T8444 ROOT released,released
R6866 T8445 T8446 advmod very,early
R6867 T8446 T8447 amod early,following
R6868 T8447 T8444 dobj following,released
R6869 T8448 T8447 pobj shock,following
R6870 T8449 T8444 cc and,released
R6871 T8450 T8451 auxpass is,considered
R6872 T8451 T8444 conj considered,released
R6873 T8452 T8454 det an,mediator
R6874 T8453 T8454 amod important,mediator
R6875 T8454 T8451 oprd mediator,considered
R6876 T8455 T8454 prep of,mediator
R6877 T8456 T8458 det the,cascade
R6878 T8457 T8458 amod inflammatory,cascade
R6879 T8458 T8455 pobj cascade,of
R6880 T8459 T8451 punct ",",considered
R6881 T8460 T8462 mark since,regulates
R6882 T8461 T8462 nsubj it,regulates
R6883 T8462 T8451 advcl regulates,considered
R6884 T8463 T8464 det the,synthesis
R6885 T8464 T8462 dobj synthesis,regulates
R6886 T8465 T8464 prep of,synthesis
R6887 T8466 T8469 amod several,cytokines
R6888 T8467 T8469 amod other,cytokines
R6889 T8468 T8469 amod critical,cytokines
R6890 T8469 T8465 pobj cytokines,of
R6891 T8470 T8469 cc and,cytokines
R6892 T8471 T8469 conj chemokines,cytokines
R6893 T8472 T8475 nummod .15,relationship
R6894 T8473 T8475 det A,relationship
R6895 T8474 T8475 amod clear,relationship
R6896 T8475 T8491 nsubjpass relationship,observed
R6897 T8476 T8475 prep between,relationship
R6898 T8477 T8479 amod increased,synthesis
R6899 T8478 T8479 compound TNF-α,synthesis
R6900 T8479 T8476 pobj synthesis,between
R6901 T8480 T8479 cc and,synthesis
R6902 T8481 T8482 det the,development
R6903 T8482 T8479 conj development,synthesis
R6904 T8483 T8482 prep of,development
R6905 T8484 T8483 pobj shock,of
R6906 T8485 T8484 cc and,shock
R6907 T8486 T8488 amod multiple,dysfunction
R6908 T8487 T8488 compound organ,dysfunction
R6909 T8488 T8484 conj dysfunction,shock
R6910 T8489 T8491 aux has,observed
R6911 T8490 T8491 auxpass been,observed
R6912 T8491 T8451 conj observed,considered
R6913 T8492 T8491 prep in,observed
R6914 T8493 T8494 compound animal,models
R6915 T8494 T8492 pobj models,in
R6916 T8495 T8494 prep of,models
R6917 T8496 T8495 pobj sepsis,of
R6918 T8497 T8496 nummod .15,sepsis
R6919 T8498 T8491 dep 17,observed
R6920 T8499 T8522 prep Because,seem
R6921 T8500 T8499 pcomp of,Because
R6922 T8501 T8502 poss its,importance
R6923 T8502 T8499 pobj importance,Because
R6924 T8503 T8502 prep in,importance
R6925 T8504 T8503 pcomp orchestrating,in
R6926 T8505 T8506 amod multiple,steps
R6927 T8506 T8504 dobj steps,orchestrating
R6928 T8507 T8506 prep of,steps
R6929 T8508 T8510 det the,response
R6930 T8509 T8510 amod inflammatory,response
R6931 T8510 T8507 pobj response,of
R6932 T8511 T8522 punct ",",seem
R6933 T8512 T8522 nsubj strategies,seem
R6934 T8513 T8512 acl aimed,strategies
R6935 T8514 T8513 prep at,aimed
R6936 T8515 T8514 pcomp decreasing,at
R6937 T8516 T8517 amod TNF-α,levels
R6938 T8517 T8515 dobj levels,decreasing
R6939 T8518 T8515 cc or,decreasing
R6940 T8519 T8515 conj attenuating,decreasing
R6941 T8520 T8521 poss its,production
R6942 T8521 T8522 nsubj production,seem
R6943 T8522 T8522 ROOT seem,seem
R6944 T8523 T8522 oprd attractive,seem
R6945 T8524 T8522 punct .,seem
R6946 T8525 T8526 compound PDE,inhibitors
R6947 T8526 T8529 nsubjpass inhibitors,shown
R6948 T8527 T8529 aux have,shown
R6949 T8528 T8529 auxpass been,shown
R6950 T8529 T8529 ROOT shown,shown
R6951 T8530 T8531 aux to,downregulate
R6952 T8531 T8529 advcl downregulate,shown
R6953 T8532 T8533 amod TNF-α,production
R6954 T8533 T8531 dobj production,downregulate
R6955 T8534 T8533 prep in,production
R6956 T8535 T8538 amod distinct,populations
R6957 T8536 T8537 amod human,cell
R6958 T8537 T8538 compound cell,populations
R6959 T8538 T8534 pobj populations,in
R6960 T8539 T8529 punct .,shown
R6961 T8540 T8543 nsubj We,shown
R6962 T8541 T8543 aux have,shown
R6963 T8542 T8543 advmod previously,shown
R6964 T8543 T8543 ROOT shown,shown
R6965 T8544 T8551 mark that,continue
R6966 T8545 T8551 punct ",",continue
R6967 T8546 T8551 prep in,continue
R6968 T8547 T8546 amod general,in
R6969 T8548 T8551 punct ",",continue
R6970 T8549 T8550 compound TNF-α,levels
R6971 T8550 T8551 nsubj levels,continue
R6972 T8551 T8543 ccomp continue,shown
R6973 T8552 T8553 aux to,increase
R6974 T8553 T8551 xcomp increase,continue
R6975 T8554 T8553 prep after,increase
R6976 T8555 T8556 compound LPS,exposure
R6977 T8556 T8554 pobj exposure,after
R6978 T8557 T8551 cc and,continue
R6979 T8558 T8566 mark that,decrease
R6980 T8559 T8560 amod concomitant,infusion
R6981 T8560 T8566 nsubj infusion,decrease
R6982 T8561 T8560 prep of,infusion
R6983 T8562 T8563 compound phosphodiesterase,inhibitors
R6984 T8563 T8561 pobj inhibitors,of
R6985 T8564 T8566 aux can,decrease
R6986 T8565 T8566 advmod markedly,decrease
R6987 T8566 T8551 conj decrease,continue
R6988 T8567 T8568 amod TNF-α,levels
R6989 T8568 T8566 dobj levels,decrease
R6990 T8569 T8568 nummod .18,levels
R6991 T8570 T8568 appos 19,levels
R6992 T8571 T8578 prep In,verified
R6993 T8572 T8571 pobj this,In
R6994 T8573 T8578 prep in,verified
R6995 T8574 T8575 amod vitro,study
R6996 T8575 T8573 pobj study,in
R6997 T8576 T8578 punct ",",verified
R6998 T8577 T8578 nsubj we,verified
R6999 T8578 T8578 ROOT verified,verified
R7000 T8579 T8581 det the,attenuation
R7001 T8580 T8581 amod sustained,attenuation
R7002 T8581 T8578 dobj attenuation,verified
R7003 T8582 T8581 prep of,attenuation
R7004 T8583 T8585 amod LPS-induced,expression
R7005 T8584 T8585 amod TNF-α,expression
R7006 T8585 T8582 pobj expression,of
R7007 T8586 T8585 prep over,expression
R7008 T8587 T8586 pobj time,over
R7009 T8588 T8578 prep in,verified
R7010 T8589 T8594 det an,population
R7011 T8590 T8594 amod isolated,population
R7012 T8591 T8594 amod human,population
R7013 T8592 T8594 compound mononuclear,population
R7014 T8593 T8594 compound cell,population
R7015 T8594 T8588 pobj population,in
R7016 T8595 T8578 punct .,verified
R7017 T8596 T8597 det The,mechanisms
R7018 T8597 T8617 nsubj mechanisms,elucidated
R7019 T8598 T8610 prep by,decreases
R7020 T8599 T8598 pobj which,by
R7021 T8600 T8610 nsubj PTX,decreases
R7022 T8601 T8600 punct ",",PTX
R7023 T8602 T8605 det a,inhibitor
R7024 T8603 T8605 amod non-specific,inhibitor
R7025 T8604 T8605 compound phosphodiesterase,inhibitor
R7026 T8605 T8600 appos inhibitor,PTX
R7027 T8606 T8600 punct ",",PTX
R7028 T8607 T8610 advmod significantly,decreases
R7029 T8608 T8607 cc and,significantly
R7030 T8609 T8607 conj consistently,significantly
R7031 T8610 T8597 relcl decreases,mechanisms
R7032 T8611 T8612 amod TNF-α,production
R7033 T8612 T8610 dobj production,decreases
R7034 T8613 T8617 aux have,elucidated
R7035 T8614 T8617 neg not,elucidated
R7036 T8615 T8617 auxpass been,elucidated
R7037 T8616 T8617 advmod completely,elucidated
R7038 T8617 T8617 ROOT elucidated,elucidated
R7039 T8618 T8617 punct .,elucidated
R7040 T8619 T8622 nsubjpass It,postulated
R7041 T8620 T8622 aux has,postulated
R7042 T8621 T8622 auxpass been,postulated
R7043 T8622 T8622 ROOT postulated,postulated
R7044 T8623 T8629 mark that,exert
R7045 T8624 T8629 nsubj drugs,exert
R7046 T8625 T8626 nsubj that,increase
R7047 T8626 T8624 relcl increase,drugs
R7048 T8627 T8628 compound intracellular,cAMP
R7049 T8628 T8626 dobj cAMP,increase
R7050 T8629 T8622 ccomp exert,postulated
R7051 T8630 T8632 poss their,effects
R7052 T8631 T8632 amod anti-inflammatory,effects
R7053 T8632 T8629 dobj effects,exert
R7054 T8633 T8629 prep through,exert
R7055 T8634 T8635 det the,activation
R7056 T8635 T8633 pobj activation,through
R7057 T8636 T8635 prep of,activation
R7058 T8637 T8636 pobj PKA,of
R7059 T8638 T8622 punct .,postulated
R7060 T8639 T8641 det This,pathway
R7061 T8640 T8641 amod classic,pathway
R7062 T8641 T8644 nsubjpass pathway,challenged
R7063 T8642 T8644 aux has,challenged
R7064 T8643 T8644 auxpass been,challenged
R7065 T8644 T8644 ROOT challenged,challenged
R7066 T8645 T8644 prep as,challenged
R7067 T8646 T8648 det the,mechanism
R7068 T8647 T8648 amod sole,mechanism
R7069 T8648 T8645 pobj mechanism,as
R7070 T8649 T8648 acl involved,mechanism
R7071 T8650 T8649 prep in,involved
R7072 T8651 T8652 det the,modulation
R7073 T8652 T8650 pobj modulation,in
R7074 T8653 T8652 prep of,modulation
R7075 T8654 T8653 pobj inflammation,of
R7076 T8655 T8649 agent by,involved
R7077 T8656 T8657 amod cAMP-enhancing,drugs
R7078 T8657 T8655 pobj drugs,by
R7079 T8658 T8657 punct ",",drugs
R7080 T8659 T8657 cc and,drugs
R7081 T8660 T8661 amod alternative,mechanisms
R7082 T8661 T8657 conj mechanisms,drugs
R7083 T8662 T8661 acl involving,mechanisms
R7084 T8663 T8664 amod PKA-independent,pathways
R7085 T8664 T8662 dobj pathways,involving
R7086 T8665 T8667 aux have,proposed
R7087 T8666 T8667 auxpass been,proposed
R7088 T8667 T8644 conj proposed,challenged
R7089 T8668 T8667 dobj .6,proposed
R7090 T8669 T8667 prep 8,proposed
R7091 T8670 T8677 prep In,observed
R7092 T8671 T8672 det this,series
R7093 T8672 T8670 pobj series,In
R7094 T8673 T8672 prep of,series
R7095 T8674 T8673 pobj experiments,of
R7096 T8675 T8677 punct ",",observed
R7097 T8676 T8677 nsubj we,observed
R7098 T8677 T8677 ROOT observed,observed
R7099 T8678 T8680 det a,attenuation
R7100 T8679 T8680 amod significant,attenuation
R7101 T8680 T8677 dobj attenuation,observed
R7102 T8681 T8680 prep of,attenuation
R7103 T8682 T8683 amod TNF-α,production
R7104 T8683 T8681 pobj production,of
R7105 T8684 T8683 prep in,production
R7106 T8685 T8688 amod LPS-stimulated,cells
R7107 T8686 T8688 amod human,cells
R7108 T8687 T8688 compound mononuclear,cells
R7109 T8688 T8684 pobj cells,in
R7110 T8689 T8688 acl following,cells
R7111 T8690 T8691 compound PTX,administration
R7112 T8691 T8689 dobj administration,following
R7113 T8692 T8691 punct ",",administration
R7114 T8693 T8691 amod independent,administration
R7115 T8694 T8693 prep of,independent
R7116 T8695 T8696 compound PKA,activation
R7117 T8696 T8694 pobj activation,of
R7118 T8697 T8677 punct .,observed
R7119 T8698 T8699 nsubj We,used
R7120 T8699 T8699 ROOT used,used
R7121 T8700 T8703 det a,inhibitor
R7122 T8701 T8703 amod nonspecific,inhibitor
R7123 T8702 T8703 compound phosphodiesterase,inhibitor
R7124 T8703 T8699 dobj inhibitor,used
R7125 T8704 T8703 punct ",",inhibitor
R7126 T8705 T8703 appos PTX,inhibitor
R7127 T8706 T8703 punct ",",inhibitor
R7128 T8707 T8699 prep in,used
R7129 T8708 T8710 det the,study
R7130 T8709 T8710 amod present,study
R7131 T8710 T8707 pobj study,in
R7132 T8711 T8715 mark because,used
R7133 T8712 T8715 nsubjpass it,used
R7134 T8713 T8715 aux has,used
R7135 T8714 T8715 auxpass been,used
R7136 T8715 T8699 advcl used,used
R7137 T8716 T8715 advmod clinically,used
R7138 T8717 T8715 prep in,used
R7139 T8718 T8719 det the,treatment
R7140 T8719 T8717 pobj treatment,in
R7141 T8720 T8719 prep of,treatment
R7142 T8721 T8722 det a,variety
R7143 T8722 T8720 pobj variety,of
R7144 T8723 T8722 prep of,variety
R7145 T8724 T8723 pobj conditions,of
R7146 T8725 T8728 prep in,is
R7147 T8726 T8725 pobj which,in
R7148 T8727 T8728 nsubj inflammation,is
R7149 T8728 T8699 conj is,used
R7150 T8729 T8731 det an,component
R7151 T8730 T8731 amod important,component
R7152 T8731 T8728 attr component,is
R7153 T8732 T8731 prep of,component
R7154 T8733 T8734 det the,pathophysiology
R7155 T8734 T8732 pobj pathophysiology,of
R7156 T8735 T8734 prep of,pathophysiology
R7157 T8736 T8738 det the,process
R7158 T8737 T8738 compound disease,process
R7159 T8738 T8735 pobj process,of
R7160 T8739 T8731 appos .19,component
R7161 T8740 T8761 advmod Additionally,explored
R7162 T8741 T8761 punct ",",explored
R7163 T8742 T8743 det the,use
R7164 T8743 T8761 nsubjpass use,explored
R7165 T8744 T8743 prep of,use
R7166 T8745 T8744 pobj PTX,of
R7167 T8746 T8743 prep in,use
R7168 T8747 T8746 pobj sepsis,in
R7169 T8748 T8743 prep as,use
R7170 T8749 T8750 det an,adjunct
R7171 T8750 T8748 pobj adjunct,as
R7172 T8751 T8750 prep to,adjunct
R7173 T8752 T8753 amod other,treatments
R7174 T8753 T8751 pobj treatments,to
R7175 T8754 T8755 aux to,maintain
R7176 T8755 T8750 relcl maintain,adjunct
R7177 T8756 T8758 amod adequate,function
R7178 T8757 T8758 compound organ,function
R7179 T8758 T8755 dobj function,maintain
R7180 T8759 T8761 aux has,explored
R7181 T8760 T8761 auxpass been,explored
R7182 T8761 T8761 ROOT explored,explored
R7183 T8762 T8761 prep based,explored
R7184 T8763 T8762 prep upon,based
R7185 T8764 T8765 poss its,effects
R7186 T8765 T8763 pobj effects,upon
R7187 T8766 T8765 prep on,effects
R7188 T8767 T8768 amod TNF-α,synthesis
R7189 T8768 T8766 pobj synthesis,on
R7190 T8769 T8761 punct .,explored
R7191 T8770 T8778 advmod However,are
R7192 T8771 T8778 punct ",",are
R7193 T8772 T8775 det the,effects
R7194 T8773 T8775 amod reported,effects
R7195 T8774 T8775 amod beneficial,effects
R7196 T8775 T8778 nsubj effects,are
R7197 T8776 T8775 prep of,effects
R7198 T8777 T8776 pobj PTX,of
R7199 T8778 T8818 ccomp are,demonstrated
R7200 T8779 T8781 preconj not,related
R7201 T8780 T8779 advmod only,not
R7202 T8781 T8778 acomp related,are
R7203 T8782 T8781 prep to,related
R7204 T8783 T8784 det the,downregulation
R7205 T8784 T8782 pobj downregulation,to
R7206 T8785 T8784 prep of,downregulation
R7207 T8786 T8785 pobj TNF-αbut,of
R7208 T8787 T8788 advmod also,to
R7209 T8788 T8781 prep to,related
R7210 T8789 T8791 poss PTX,properties
R7211 T8790 T8791 amod hemorrheologic,properties
R7212 T8791 T8788 pobj properties,to
R7213 T8792 T8791 punct ",",properties
R7214 T8793 T8794 poss its,ability
R7215 T8794 T8788 pobj ability,to
R7216 T8795 T8796 aux to,reduce
R7217 T8796 T8794 acl reduce,ability
R7218 T8797 T8798 compound neutrophil,activation
R7219 T8798 T8796 dobj activation,reduce
R7220 T8799 T8781 punct ",",related
R7221 T8800 T8781 cc and,related
R7222 T8801 T8803 poss its,effects
R7223 T8802 T8803 amod beneficial,effects
R7224 T8803 T8778 attr effects,are
R7225 T8804 T8803 prep on,effects
R7226 T8805 T8804 pobj microcirculation,on
R7227 T8806 T8805 punct ",",microcirculation
R7228 T8807 T8808 amod cardiac,performance
R7229 T8808 T8805 conj performance,microcirculation
R7230 T8809 T8808 punct ",",performance
R7231 T8810 T8808 cc and,performance
R7232 T8811 T8812 compound organ,injury
R7233 T8812 T8813 compound injury,.20
R7234 T8813 T8808 conj .20,performance
R7235 T8814 T8815 nummod 25,Herein
R7236 T8815 T8813 appos Herein,.20
R7237 T8816 T8818 punct ",",demonstrated
R7238 T8817 T8818 nsubj we,demonstrated
R7239 T8818 T8818 ROOT demonstrated,demonstrated
R7240 T8819 T8829 mark that,involves
R7241 T8820 T8822 det the,effects
R7242 T8821 T8822 amod downregulatory,effects
R7243 T8822 T8829 nsubj effects,involves
R7244 T8823 T8822 prep of,effects
R7245 T8824 T8823 pobj PTX,of
R7246 T8825 T8822 prep in,effects
R7247 T8826 T8828 amod human,cells
R7248 T8827 T8828 compound mononuclear,cells
R7249 T8828 T8825 pobj cells,in
R7250 T8829 T8818 ccomp involves,demonstrated
R7251 T8830 T8829 dobj modulation,involves
R7252 T8831 T8830 prep of,modulation
R7253 T8832 T8831 pobj pathways,of
R7254 T8833 T8834 nsubj that,involve
R7255 T8834 T8832 relcl involve,pathways
R7256 T8835 T8834 dobj NF-κB,involve
R7257 T8836 T8835 cc and,NF-κB
R7258 T8837 T8835 conj CREB,NF-κB
R7259 T8838 T8837 punct ",",CREB
R7260 T8839 T8842 nummod two,factors
R7261 T8840 T8842 amod major,factors
R7262 T8841 T8842 compound transcription,factors
R7263 T8842 T8830 conj factors,modulation
R7264 T8843 T8842 acl involved,factors
R7265 T8844 T8843 prep in,involved
R7266 T8845 T8847 det the,cascade
R7267 T8846 T8847 amod inflammatory,cascade
R7268 T8847 T8844 pobj cascade,in
R7269 T8848 T8818 punct .,demonstrated
R7270 T8849 T8851 compound NF-κB,Rel
R7271 T8850 T8851 compound /,Rel
R7272 T8851 T8852 nsubj Rel,constitutes
R7273 T8852 T8852 ROOT constitutes,constitutes
R7274 T8853 T8854 det a,family
R7275 T8854 T8852 dobj family,constitutes
R7276 T8855 T8854 prep of,family
R7277 T8856 T8857 amod transcriptional,factors
R7278 T8857 T8855 pobj factors,of
R7279 T8858 T8857 acl involved,factors
R7280 T8859 T8858 prep in,involved
R7281 T8860 T8861 det the,regulation
R7282 T8861 T8859 pobj regulation,in
R7283 T8862 T8861 prep of,regulation
R7284 T8863 T8865 amod numerous,genes
R7285 T8864 T8865 compound cytokine,genes
R7286 T8865 T8862 pobj genes,of
R7287 T8866 T8865 cc and,genes
R7288 T8867 T8868 amod immune,responses
R7289 T8868 T8865 conj responses,genes
R7290 T8869 T8868 prep in,responses
R7291 T8870 T8872 amod different,populations
R7292 T8871 T8872 compound cell,populations
R7293 T8872 T8869 pobj populations,in
R7294 T8873 T8852 punct .,constitutes
R7295 T8874 T8877 det The,form
R7296 T8875 T8876 advmod most,abundant
R7297 T8876 T8877 amod abundant,form
R7298 T8877 T8880 nsubj form,is
R7299 T8878 T8877 prep of,form
R7300 T8879 T8878 pobj NF-κB,of
R7301 T8880 T8880 ROOT is,is
R7302 T8881 T8882 det the,p50
R7303 T8882 T8880 attr p50,is
R7304 T8883 T8885 quantmod p65,.26
R7305 T8884 T8885 quantmod heterodimer,.26
R7306 T8885 T8882 appos .26,p50
R7307 T8886 T8888 det The,form
R7308 T8887 T8888 amod inactive,form
R7309 T8888 T8891 nsubj form,exists
R7310 T8889 T8888 prep of,form
R7311 T8890 T8891 compound NF-κB,exists
R7312 T8891 T8891 ROOT exists,exists
R7313 T8892 T8891 prep in,exists
R7314 T8893 T8894 det the,cytoplasm
R7315 T8894 T8892 pobj cytoplasm,in
R7316 T8895 T8891 advcl bound,exists
R7317 T8896 T8895 prep to,bound
R7318 T8897 T8900 det an,containing
R7319 T8898 T8899 advmod inhibitory,complex
R7320 T8899 T8900 amod complex,containing
R7321 T8900 T8891 advcl containing,exists
R7322 T8901 T8900 dobj I-κBα,containing
R7323 T8902 T8891 punct .,exists
R7324 T8903 T8917 csubj Following,activates
R7325 T8904 T8905 amod cellular,stimulation
R7326 T8905 T8903 pobj stimulation,Following
R7327 T8906 T8905 prep with,stimulation
R7328 T8907 T8906 pobj LPS,with
R7329 T8908 T8907 cc and,LPS
R7330 T8909 T8910 amod chemotactic,factors
R7331 T8910 T8907 conj factors,LPS
R7332 T8911 T8910 punct ",",factors
R7333 T8912 T8915 amod NF-κB,phosphorylates
R7334 T8913 T8915 amod inducible,phosphorylates
R7335 T8914 T8915 compound kinase,phosphorylates
R7336 T8915 T8910 conj phosphorylates,factors
R7337 T8916 T8915 cc and,phosphorylates
R7338 T8917 T8917 ROOT activates,activates
R7339 T8918 T8920 det the,kinase
R7340 T8919 T8920 amod I-κB,kinase
R7341 T8920 T8917 dobj kinase,activates
R7342 T8921 T8920 punct (,kinase
R7343 T8922 T8920 appos IKK,kinase
R7344 T8923 T8920 punct ),kinase
R7345 T8924 T8925 amod complex,consisting
R7346 T8925 T8917 advcl consisting,activates
R7347 T8926 T8925 prep of,consisting
R7348 T8927 T8926 pobj IKK-1,of
R7349 T8928 T8927 cc and,IKK-1
R7350 T8929 T8927 conj IKK-2,IKK-1
R7351 T8930 T8927 punct ",",IKK-1
R7352 T8931 T8934 nsubj which,phosphorylates
R7353 T8932 T8934 prep in,phosphorylates
R7354 T8933 T8932 pobj turn,in
R7355 T8934 T8927 relcl phosphorylates,IKK-1
R7356 T8935 T8937 det the,subunit
R7357 T8936 T8937 amod I-κBα,subunit
R7358 T8937 T8934 dobj subunit,phosphorylates
R7359 T8938 T8917 punct .,activates
R7360 T8939 T8939 ROOT Phosphorylation,Phosphorylation
R7361 T8940 T8939 punct ",",Phosphorylation
R7362 T8941 T8939 conj ubiquitination,Phosphorylation
R7363 T8942 T8941 punct ",",ubiquitination
R7364 T8943 T8941 cc and,ubiquitination
R7365 T8944 T8945 amod subsequent,degradation
R7366 T8945 T8941 conj degradation,ubiquitination
R7367 T8946 T8945 prep of,degradation
R7368 T8947 T8948 amod I-κBα,results
R7369 T8948 T8946 pobj results,of
R7370 T8949 T8948 prep in,results
R7371 T8950 T8952 amod NF-κB,translocation
R7372 T8951 T8952 amod nuclear,translocation
R7373 T8952 T8949 pobj translocation,in
R7374 T8953 T8939 punct .,Phosphorylation
R7375 T8954 T8955 nsubj This,allows
R7376 T8955 T8955 ROOT allows,allows
R7377 T8956 T8958 det the,factor
R7378 T8957 T8958 compound transcription,factor
R7379 T8958 T8960 nsubj factor,bind
R7380 T8959 T8960 aux to,bind
R7381 T8960 T8955 ccomp bind,allows
R7382 T8961 T8962 aux to,promoter
R7383 T8962 T8960 xcomp promoter,bind
R7384 T8963 T8962 dobj regions,promoter
R7385 T8964 T8962 prep of,promoter
R7386 T8965 T8967 amod specific,genes
R7387 T8966 T8967 amod pro-inflammatory,genes
R7388 T8967 T8964 pobj genes,of
R7389 T8968 T8967 cc and,genes
R7390 T8969 T8970 compound influence,transcription
R7391 T8970 T8962 conj transcription,promoter
R7392 T8971 T8970 nummod ".27,28",transcription
R7393 T8972 T8979 mark Because,are
R7394 T8973 T8974 det a,number
R7395 T8974 T8979 nsubj number,are
R7396 T8975 T8974 prep of,number
R7397 T8976 T8978 amod potential,sites
R7398 T8977 T8978 compound κB,sites
R7399 T8978 T8975 pobj sites,of
R7400 T8979 T8992 advcl are,considered
R7401 T8980 T8979 acomp present,are
R7402 T8981 T8980 prep in,present
R7403 T8982 T8984 det the,sequences
R7404 T8983 T8984 compound nucleotide,sequences
R7405 T8984 T8981 pobj sequences,in
R7406 T8985 T8984 prep of,sequences
R7407 T8986 T8988 det the,gene
R7408 T8987 T8988 compound TNF-α,gene
R7409 T8988 T8985 pobj gene,of
R7410 T8989 T8992 punct ",",considered
R7411 T8990 T8992 nsubjpass NF-κB,considered
R7412 T8991 T8992 auxpass is,considered
R7413 T8992 T8992 ROOT considered,considered
R7414 T8993 T8994 aux to,be
R7415 T8994 T8992 xcomp be,considered
R7416 T8995 T8998 det a,factor
R7417 T8996 T8998 amod critical,factor
R7418 T8997 T8998 compound transcriptional,factor
R7419 T8998 T8994 attr factor,be
R7420 T8999 T8998 acl involved,factor
R7421 T9000 T8999 prep in,involved
R7422 T9001 T9003 compound TNF-α,expression
R7423 T9002 T9003 compound gene,expression
R7424 T9003 T9000 pobj expression,in
R7425 T9004 T9003 cc and,expression
R7426 T9005 T9003 conj protein,expression
R7427 T9006 T9003 conj synthesis,expression
R7428 T9007 T9006 nummod ".29,30",synthesis
R7429 T9008 T9015 prep In,demonstrated
R7430 T9009 T9010 det this,set
R7431 T9010 T9008 pobj set,In
R7432 T9011 T9010 prep of,set
R7433 T9012 T9011 pobj experiments,of
R7434 T9013 T9015 punct ",",demonstrated
R7435 T9014 T9015 nsubj we,demonstrated
R7436 T9015 T9015 ROOT demonstrated,demonstrated
R7437 T9016 T9018 mark that,downregulates
R7438 T9017 T9018 nsubj PTX,downregulates
R7439 T9018 T9015 ccomp downregulates,demonstrated
R7440 T9019 T9021 nmod cytoplasmic,phosphorylation
R7441 T9020 T9021 amod I-κBα,phosphorylation
R7442 T9021 T9018 dobj phosphorylation,downregulates
R7443 T9022 T9021 punct ",",phosphorylation
R7444 T9023 T9026 amod nuclear,phosphorylation/translocation
R7445 T9024 T9026 nmod NF-κB,phosphorylation/translocation
R7446 T9025 T9026 nummod p65,phosphorylation/translocation
R7447 T9026 T9021 appos phosphorylation/translocation,phosphorylation
R7448 T9027 T9026 punct ",",phosphorylation/translocation
R7449 T9028 T9030 advmod as,as
R7450 T9029 T9030 advmod well,as
R7451 T9030 T9026 cc as,phosphorylation/translocation
R7452 T9031 T9032 compound NF-κB,DNA-binding
R7453 T9032 T9033 compound DNA-binding,after
R7454 T9033 T9018 prep after,downregulates
R7455 T9034 T9035 compound LPS,stimulation
R7456 T9035 T9033 pobj stimulation,after
R7457 T9036 T9018 punct ",",downregulates
R7458 T9037 T9018 advcl suggesting,downregulates
R7459 T9038 T9040 mark that,exerts
R7460 T9039 T9040 nsubj PTX,exerts
R7461 T9040 T9037 ccomp exerts,suggesting
R7462 T9041 T9043 poss its,proximal
R7463 T9042 T9043 compound function,proximal
R7464 T9043 T9040 dobj proximal,exerts
R7465 T9044 T9043 prep to,proximal
R7466 T9045 T9044 cc or,to
R7467 T9046 T9040 prep at,exerts
R7468 T9047 T9048 det the,level
R7469 T9048 T9046 pobj level,at
R7470 T9049 T9048 prep of,level
R7471 T9050 T9051 amod I-κBα,phosphorylation
R7472 T9051 T9049 pobj phosphorylation,of
R7473 T9052 T9015 punct .,demonstrated
R7474 T9053 T9054 amod Previous,studies
R7475 T9054 T9068 nsubj studies,reported
R7476 T9055 T9057 nsubj that,evaluated
R7477 T9056 T9057 aux have,evaluated
R7478 T9057 T9054 relcl evaluated,studies
R7479 T9058 T9059 det the,effects
R7480 T9059 T9057 dobj effects,evaluated
R7481 T9060 T9059 prep of,effects
R7482 T9061 T9062 amod cAMP-elevating,drugs
R7483 T9062 T9060 pobj drugs,of
R7484 T9063 T9059 prep on,effects
R7485 T9064 T9063 pobj I-κBα,on
R7486 T9065 T9064 cc and,I-κBα
R7487 T9066 T9064 conj NF-κB,I-κBα
R7488 T9067 T9068 aux have,reported
R7489 T9068 T9068 ROOT reported,reported
R7490 T9069 T9070 amod conflicting,results
R7491 T9070 T9068 dobj results,reported
R7492 T9071 T9068 punct .,reported
R7493 T9072 T9074 compound Haddad,al.
R7494 T9073 T9074 compound et,al.
R7495 T9074 T9075 nsubj al.,reported
R7496 T9075 T9075 ROOT reported,reported
R7497 T9076 T9075 dobj results,reported
R7498 T9077 T9076 amod similar,results
R7499 T9078 T9077 prep to,similar
R7500 T9079 T9078 pobj ours,to
R7501 T9080 T9076 prep with,results
R7502 T9081 T9080 pobj respect,with
R7503 T9082 T9081 prep to,respect
R7504 T9083 T9084 det the,effects
R7505 T9084 T9082 pobj effects,to
R7506 T9085 T9084 prep of,effects
R7507 T9086 T9085 pobj PTX,of
R7508 T9087 T9084 prep on,effects
R7509 T9088 T9087 pobj I-κBα,on
R7510 T9089 T9088 cc and,I-κBα
R7511 T9090 T9088 conj NF-κB,I-κBα
R7512 T9091 T9084 prep in,effects
R7513 T9092 T9094 amod pulmonary,cells
R7514 T9093 T9094 amod epithelial,cells
R7515 T9094 T9091 pobj cells,in
R7516 T9095 T9084 appos .31,effects
R7517 T9096 T9101 advmod Conversely,proposed
R7518 T9097 T9101 punct ",",proposed
R7519 T9098 T9100 compound Neumann,al.
R7520 T9099 T9100 compound et,al.
R7521 T9100 T9101 nsubj al.,proposed
R7522 T9101 T9101 ROOT proposed,proposed
R7523 T9102 T9106 mark that,stabilizes
R7524 T9103 T9106 advcl increased,stabilizes
R7525 T9104 T9105 compound intracellular,cAMP
R7526 T9105 T9106 nsubj cAMP,stabilizes
R7527 T9106 T9101 ccomp stabilizes,proposed
R7528 T9107 T9108 det the,interaction
R7529 T9108 T9106 dobj interaction,stabilizes
R7530 T9109 T9108 prep between,interaction
R7531 T9110 T9109 pobj I-κBα,between
R7532 T9111 T9110 cc and,I-κBα
R7533 T9112 T9110 conj NF-κB,I-κBα
R7534 T9113 T9106 punct ",",stabilizes
R7535 T9114 T9115 advmod therefore,reducing
R7536 T9115 T9106 advcl reducing,stabilizes
R7537 T9116 T9117 amod NF-κB,activation
R7538 T9117 T9115 dobj activation,reducing
R7539 T9118 T9115 npadvmod .32,reducing
R7540 T9119 T9125 prep In,showed
R7541 T9120 T9119 pobj contrast,In
R7542 T9121 T9125 punct ",",showed
R7543 T9122 T9124 compound Takahashi,al.
R7544 T9123 T9124 compound et,al.
R7545 T9124 T9125 nsubj al.,showed
R7546 T9125 T9125 ROOT showed,showed
R7547 T9126 T9127 det no,difference
R7548 T9127 T9125 dobj difference,showed
R7549 T9128 T9127 prep in,difference
R7550 T9129 T9130 amod I-κBα,degradation
R7551 T9130 T9128 pobj degradation,in
R7552 T9131 T9130 prep in,degradation
R7553 T9132 T9133 compound Jurkat,T-lymphocytes
R7554 T9133 T9131 pobj T-lymphocytes,in
R7555 T9134 T9127 prep in,difference
R7556 T9135 T9136 det the,presence
R7557 T9136 T9134 pobj presence,in
R7558 T9137 T9136 cc and,presence
R7559 T9138 T9136 conj absence,presence
R7560 T9139 T9138 prep of,absence
R7561 T9140 T9139 pobj forskolin,of
R7562 T9141 T9127 punct ",",difference
R7563 T9142 T9146 det an,compound
R7564 T9143 T9146 amod alternative,compound
R7565 T9144 T9145 nsubj cAMP,inducing
R7566 T9145 T9146 amod inducing,compound
R7567 T9146 T9127 appos compound,difference
R7568 T9147 T9125 punct .,showed
R7569 T9148 T9149 det This,group
R7570 T9149 T9151 nsubj group,demonstrated
R7571 T9150 T9151 advmod also,demonstrated
R7572 T9151 T9151 ROOT demonstrated,demonstrated
R7573 T9152 T9156 mark that,inhibit
R7574 T9153 T9156 nsubj forskolin,inhibit
R7575 T9154 T9156 aux did,inhibit
R7576 T9155 T9156 neg not,inhibit
R7577 T9156 T9151 ccomp inhibit,demonstrated
R7578 T9157 T9159 det the,activity
R7579 T9158 T9159 amod DNA-binding,activity
R7580 T9159 T9156 dobj activity,inhibit
R7581 T9160 T9159 prep of,activity
R7582 T9161 T9160 pobj NF-κB,of
R7583 T9162 T9156 cc and,inhibit
R7584 T9163 T9167 mark that,involved
R7585 T9164 T9167 nsubjpass CREB,involved
R7586 T9165 T9167 auxpass was,involved
R7587 T9166 T9167 neg not,involved
R7588 T9167 T9156 conj involved,inhibit
R7589 T9168 T9167 prep in,involved
R7590 T9169 T9170 amod forskolin-induced,decrease
R7591 T9170 T9168 pobj decrease,in
R7592 T9171 T9170 prep in,decrease
R7593 T9172 T9174 nummod p65,activity
R7594 T9173 T9174 amod transcriptional,activity
R7595 T9174 T9171 pobj activity,in
R7596 T9175 T9177 nummod .33,results
R7597 T9176 T9177 amod Similar,results
R7598 T9177 T9179 nsubjpass results,reported
R7599 T9178 T9179 auxpass were,reported
R7600 T9179 T9151 conj reported,demonstrated
R7601 T9180 T9179 agent by,reported
R7602 T9181 T9180 pobj Ollivier,by
R7603 T9182 T9183 nmod et,al.
R7604 T9183 T9184 npadvmod al.,using
R7605 T9184 T9179 advcl using,reported
R7606 T9185 T9186 nummod THP-1,cells
R7607 T9186 T9184 dobj cells,using
R7608 T9187 T9186 cc and,cells
R7609 T9188 T9189 amod endothelial,cells
R7610 T9189 T9186 conj cells,cells
R7611 T9190 T9186 acl treated,cells
R7612 T9191 T9190 prep with,treated
R7613 T9192 T9191 pobj forskolin,with
R7614 T9193 T9192 cc or,forskolin
R7615 T9194 T9195 compound dibutyryl,cAMP
R7616 T9195 T9192 conj cAMP,forskolin
R7617 T9196 T9195 punct ",",cAMP
R7618 T9197 T9198 nummod two,agents
R7619 T9198 T9195 appos agents,cAMP
R7620 T9199 T9198 acl known,agents
R7621 T9200 T9201 aux to,function
R7622 T9201 T9199 xcomp function,known
R7623 T9202 T9201 prep through,function
R7624 T9203 T9204 compound cAMP,elevation
R7625 T9204 T9202 pobj elevation,through
R7626 T9205 T9204 cc and,elevation
R7627 T9206 T9208 amod subsequent,activation
R7628 T9207 T9208 compound PKA,activation
R7629 T9208 T9204 conj activation,elevation
R7630 T9209 T9208 nummod .9,activation
R7631 T9210 T9211 det The,differences
R7632 T9211 T9201 dobj differences,function
R7633 T9212 T9211 prep between,differences
R7634 T9213 T9214 poss our,results
R7635 T9214 T9212 pobj results,between
R7636 T9215 T9214 cc and,results
R7637 T9216 T9214 conj those,results
R7638 T9217 T9216 acl mentioned,those
R7639 T9218 T9217 advmod above,mentioned
R7640 T9219 T9221 aux may,explained
R7641 T9220 T9221 auxpass be,explained
R7642 T9221 T9151 conj explained,demonstrated
R7643 T9222 T9221 agent by,explained
R7644 T9223 T9224 det the,use
R7645 T9224 T9222 pobj use,by
R7646 T9225 T9224 prep of,use
R7647 T9226 T9228 amod different,types
R7648 T9227 T9228 compound cell,types
R7649 T9228 T9225 pobj types,of
R7650 T9229 T9228 cc and,types
R7651 T9230 T9231 amod exogenous,stimulants
R7652 T9231 T9228 conj stimulants,types
R7653 T9232 T9151 punct .,demonstrated
R7654 T9233 T9234 nsubj It,is
R7655 T9234 T9234 ROOT is,is
R7656 T9235 T9234 advmod also,is
R7657 T9236 T9234 acomp possible,is
R7658 T9237 T9239 mark that,exerts
R7659 T9238 T9239 nsubj PTX,exerts
R7660 T9239 T9234 ccomp exerts,is
R7661 T9240 T9242 poss its,effects
R7662 T9241 T9242 amod anti-inflammatory,effects
R7663 T9242 T9239 dobj effects,exerts
R7664 T9243 T9242 prep on,effects
R7665 T9244 T9246 det the,pathway
R7666 T9245 T9246 compound NF-κB,pathway
R7667 T9246 T9243 pobj pathway,on
R7668 T9247 T9246 amod independent,pathway
R7669 T9248 T9247 prep of,independent
R7670 T9249 T9248 pobj PKA,of
R7671 T9250 T9239 punct ",",exerts
R7672 T9251 T9252 mark as,suggested
R7673 T9252 T9239 advcl suggested,exerts
R7674 T9253 T9252 agent by,suggested
R7675 T9254 T9256 det the,attenuation
R7676 T9255 T9256 amod continued,attenuation
R7677 T9256 T9253 pobj attenuation,by
R7678 T9257 T9256 prep of,attenuation
R7679 T9258 T9260 amod LPS-induced,production
R7680 T9259 T9260 compound TNF-α,production
R7681 T9260 T9257 pobj production,of
R7682 T9261 T9260 prep by,production
R7683 T9262 T9261 pobj PTX,by
R7684 T9263 T9256 prep in,attenuation
R7685 T9264 T9265 det the,presence
R7686 T9265 T9263 pobj presence,in
R7687 T9266 T9265 cc or,presence
R7688 T9267 T9265 conj absence,presence
R7689 T9268 T9267 prep of,absence
R7690 T9269 T9270 compound PKA,inhibition
R7691 T9270 T9268 pobj inhibition,of
R7692 T9271 T9234 advcl observed,is
R7693 T9272 T9271 prep in,observed
R7694 T9273 T9274 det this,study
R7695 T9274 T9272 pobj study,in
R7696 T9275 T9234 punct .,is
R7697 T9276 T9277 det This,hypothesis
R7698 T9277 T9278 nsubj hypothesis,is
R7699 T9278 T9278 ROOT is,is
R7700 T9279 T9278 advmod currently,is
R7701 T9280 T9278 prep under,is
R7702 T9281 T9280 pobj investigation,under
R7703 T9282 T9281 prep in,investigation
R7704 T9283 T9284 poss our,laboratory
R7705 T9284 T9282 pobj laboratory,in
R7706 T9285 T9278 punct .,is
R7707 T9286 T9289 advmod Furthermore,utilized
R7708 T9287 T9289 punct ",",utilized
R7709 T9288 T9289 nsubj we,utilized
R7710 T9289 T9289 ROOT utilized,utilized
R7711 T9290 T9293 det a,inhibitor
R7712 T9291 T9293 amod broad,inhibitor
R7713 T9292 T9293 compound PKA,inhibitor
R7714 T9293 T9289 dobj inhibitor,utilized
R7715 T9294 T9289 prep in,utilized
R7716 T9295 T9296 det this,study
R7717 T9296 T9294 pobj study,in
R7718 T9297 T9289 prep in,utilized
R7719 T9298 T9297 pobj addition,in
R7720 T9299 T9298 prep to,addition
R7721 T9300 T9299 pobj H89,to
R7722 T9301 T9289 punct ",",utilized
R7723 T9302 T9289 cc but,utilized
R7724 T9303 T9305 aux did,examine
R7725 T9304 T9305 neg not,examine
R7726 T9305 T9289 conj examine,utilized
R7727 T9306 T9307 det the,effect
R7728 T9307 T9305 dobj effect,examine
R7729 T9308 T9307 prep of,effect
R7730 T9309 T9310 amod specific,inhibition
R7731 T9310 T9308 pobj inhibition,of
R7732 T9311 T9310 prep of,inhibition
R7733 T9312 T9314 amod various,isoforms
R7734 T9313 T9314 compound PKA,isoforms
R7735 T9314 T9311 pobj isoforms,of
R7736 T9315 T9289 punct .,utilized
R7737 T9316 T9317 det This,information
R7738 T9317 T9319 nsubj information,yield
R7739 T9318 T9319 aux may,yield
R7740 T9319 T9319 ROOT yield,yield
R7741 T9320 T9321 amod more,insight
R7742 T9321 T9319 dobj insight,yield
R7743 T9322 T9321 prep into,insight
R7744 T9323 T9324 det the,mechanism
R7745 T9324 T9322 pobj mechanism,into
R7746 T9325 T9328 prep by,exerts
R7747 T9326 T9325 pobj which,by
R7748 T9327 T9328 nsubj PTX,exerts
R7749 T9328 T9324 relcl exerts,mechanism
R7750 T9329 T9331 poss its,effects
R7751 T9330 T9331 amod down-regulatory,effects
R7752 T9331 T9328 dobj effects,exerts
R7753 T9332 T9319 punct .,yield
R7754 T9333 T9334 compound PDE,inhibitors
R7755 T9334 T9337 nsubj inhibitors,downregulate
R7756 T9335 T9337 aux may,downregulate
R7757 T9336 T9337 advmod also,downregulate
R7758 T9337 T9337 ROOT downregulate,downregulate
R7759 T9338 T9340 amod NF-κB,activity
R7760 T9339 T9340 amod transcriptional,activity
R7761 T9340 T9337 dobj activity,downregulate
R7762 T9341 T9337 prep by,downregulate
R7763 T9342 T9341 pcomp altering,by
R7764 T9343 T9345 det the,binding
R7765 T9344 T9345 amod competitive,binding
R7766 T9345 T9342 dobj binding,altering
R7767 T9346 T9345 prep of,binding
R7768 T9347 T9346 pobj CREB,of
R7769 T9348 T9347 cc and,CREB
R7770 T9349 T9347 conj NF-κB,CREB
R7771 T9350 T9342 prep to,altering
R7772 T9351 T9353 det the,regions
R7773 T9352 T9353 compound promoter,regions
R7774 T9353 T9350 pobj regions,to
R7775 T9354 T9353 prep of,regions
R7776 T9355 T9356 amod pro-inflammatory,genes
R7777 T9356 T9354 pobj genes,of
R7778 T9357 T9337 punct .,downregulate
R7779 T9358 T9361 npadvmod Herein,showed
R7780 T9359 T9361 punct ",",showed
R7781 T9360 T9361 nsubj we,showed
R7782 T9361 T9361 ROOT showed,showed
R7783 T9362 T9371 mark that,is
R7784 T9363 T9364 det the,activation
R7785 T9364 T9368 nmod activation,activity
R7786 T9365 T9364 cc and,activation
R7787 T9366 T9364 conj DNA,activation
R7788 T9367 T9368 amod binding,activity
R7789 T9368 T9371 nsubj activity,is
R7790 T9369 T9368 prep of,activity
R7791 T9370 T9369 pobj CREB,of
R7792 T9371 T9361 ccomp is,showed
R7793 T9372 T9371 acomp upregulated,is
R7794 T9373 T9372 prep in,upregulated
R7795 T9374 T9375 det a,dose
R7796 T9375 T9373 pobj dose,in
R7797 T9376 T9377 amod dependent,manner
R7798 T9377 T9372 appos manner,upregulated
R7799 T9378 T9372 agent by,upregulated
R7800 T9379 T9378 pobj PTX,by
R7801 T9380 T9361 punct .,showed
R7802 T9381 T9391 prep Of,alleviated
R7803 T9382 T9381 pobj note,Of
R7804 T9383 T9391 punct ",",alleviated
R7805 T9384 T9385 det the,increase
R7806 T9385 T9391 nsubjpass increase,alleviated
R7807 T9386 T9385 prep in,increase
R7808 T9387 T9388 compound CREB,activity
R7809 T9388 T9386 pobj activity,in
R7810 T9389 T9391 auxpass was,alleviated
R7811 T9390 T9391 advmod slightly,alleviated
R7812 T9391 T9391 ROOT alleviated,alleviated
R7813 T9392 T9391 agent by,alleviated
R7814 T9393 T9394 det the,addition
R7815 T9394 T9392 pobj addition,by
R7816 T9395 T9394 prep of,addition
R7817 T9396 T9395 pobj LPS,of
R7818 T9397 T9391 punct ",",alleviated
R7819 T9398 T9391 advcl suggesting,alleviated
R7820 T9399 T9398 dobj competition,suggesting
R7821 T9400 T9399 prep between,competition
R7822 T9401 T9404 amod PTX-induced,transcription
R7823 T9402 T9404 amod CREB-associated,transcription
R7824 T9403 T9404 compound gene,transcription
R7825 T9404 T9400 pobj transcription,between
R7826 T9405 T9404 cc and,transcription
R7827 T9406 T9407 det those,factors
R7828 T9407 T9404 conj factors,transcription
R7829 T9408 T9407 punct ",",factors
R7830 T9409 T9410 amod such,as
R7831 T9410 T9407 prep as,factors
R7832 T9411 T9410 pobj NF-κB,as
R7833 T9412 T9411 punct ",",NF-κB
R7834 T9413 T9416 nsubjpass which,upregulated
R7835 T9414 T9416 aux may,upregulated
R7836 T9415 T9416 auxpass be,upregulated
R7837 T9416 T9411 relcl upregulated,NF-κB
R7838 T9417 T9416 agent by,upregulated
R7839 T9418 T9419 compound LPS,exposure
R7840 T9419 T9417 pobj exposure,by
R7841 T9420 T9391 punct .,alleviated
R7842 T9421 T9422 det This,competition
R7843 T9422 T9425 nsubjpass competition,postulated
R7844 T9423 T9425 aux has,postulated
R7845 T9424 T9425 auxpass been,postulated
R7846 T9425 T9425 ROOT postulated,postulated
R7847 T9426 T9427 aux to,involve
R7848 T9427 T9425 xcomp involve,postulated
R7849 T9428 T9429 det the,recruitment
R7850 T9429 T9427 dobj recruitment,involve
R7851 T9430 T9429 prep of,recruitment
R7852 T9431 T9433 det the,CBP
R7853 T9432 T9433 compound co-activator,CBP
R7854 T9433 T9430 pobj CBP,of
R7855 T9434 T9433 cc and,CBP
R7856 T9435 T9436 poss its,homologue
R7857 T9436 T9433 conj homologue,CBP
R7858 T9437 T9436 appos p30034,homologue
R7859 T9438 T9425 punct ",",postulated
R7860 T9439 T9425 advcl suggesting,postulated
R7861 T9440 T9442 det an,mechanism
R7862 T9441 T9442 amod alternative,mechanism
R7863 T9442 T9439 dobj mechanism,suggesting
R7864 T9443 T9446 nsubjpass that,involved
R7865 T9444 T9446 aux may,involved
R7866 T9445 T9446 auxpass be,involved
R7867 T9446 T9442 relcl involved,mechanism
R7868 T9447 T9446 prep in,involved
R7869 T9448 T9450 det the,actions
R7870 T9449 T9450 amod anti-inflammatory,actions
R7871 T9450 T9447 pobj actions,in
R7872 T9451 T9450 prep of,actions
R7873 T9452 T9451 pobj PTX,of
R7874 T9453 T9425 punct .,postulated
R7875 T9454 T9455 amod Future,studies
R7876 T9455 T9457 nsubjpass studies,planned
R7877 T9456 T9457 auxpass are,planned
R7878 T9457 T9457 ROOT planned,planned
R7879 T9458 T9459 aux to,delineate
R7880 T9459 T9457 xcomp delineate,planned
R7881 T9460 T9461 det the,validity
R7882 T9461 T9459 dobj validity,delineate
R7883 T9462 T9461 prep of,validity
R7884 T9463 T9465 det this,mechanism
R7885 T9464 T9465 amod potential,mechanism
R7886 T9465 T9462 pobj mechanism,of
R7887 T9466 T9457 punct .,planned
R7888 T9467 T9468 det The,effects
R7889 T9468 T9488 nsubjpass effects,ruled
R7890 T9469 T9468 prep of,effects
R7891 T9470 T9469 pobj PTX,of
R7892 T9471 T9468 prep on,effects
R7893 T9472 T9474 amod other,factors
R7894 T9473 T9474 compound transcription,factors
R7895 T9474 T9471 pobj factors,on
R7896 T9475 T9474 punct ",",factors
R7897 T9476 T9477 amod such,as
R7898 T9477 T9474 prep as,factors
R7899 T9478 T9477 pobj AP-1,as
R7900 T9479 T9478 punct ",",AP-1
R7901 T9480 T9478 conj c-fos,AP-1
R7902 T9481 T9480 punct ",",c-fos
R7903 T9482 T9480 cc and,c-fos
R7904 T9483 T9480 conj c-jun,c-fos
R7905 T9484 T9488 punct ",",ruled
R7906 T9485 T9488 aux can,ruled
R7907 T9486 T9488 neg not,ruled
R7908 T9487 T9488 auxpass be,ruled
R7909 T9488 T9488 ROOT ruled,ruled
R7910 T9489 T9488 prt out,ruled
R7911 T9490 T9488 agent by,ruled
R7912 T9491 T9492 poss our,experiments
R7913 T9492 T9490 pobj experiments,by
R7914 T9493 T9488 punct .,ruled
R7915 T9494 T9514 nsubjpass Activation,implicated
R7916 T9495 T9494 prep of,Activation
R7917 T9496 T9495 pobj AP-1,of
R7918 T9497 T9496 cc and,AP-1
R7919 T9498 T9499 poss its,components
R7920 T9499 T9496 conj components,AP-1
R7921 T9500 T9499 punct (,components
R7922 T9501 T9499 appos c-fos,components
R7923 T9502 T9501 cc and,c-fos
R7924 T9503 T9501 conj c-jun,c-fos
R7925 T9504 T9503 punct ),c-jun
R7926 T9505 T9494 agent by,Activation
R7927 T9506 T9505 pobj agents,by
R7928 T9507 T9508 nsubj that,enhance
R7929 T9508 T9506 relcl enhance,agents
R7930 T9509 T9511 amod mononuclear,activity
R7931 T9510 T9511 compound cell,activity
R7932 T9511 T9508 dobj activity,enhance
R7933 T9512 T9514 aux has,implicated
R7934 T9513 T9514 auxpass been,implicated
R7935 T9514 T9514 ROOT implicated,implicated
R7936 T9515 T9514 prep in,implicated
R7937 T9516 T9517 amod TNF-α,expression
R7938 T9517 T9515 pobj expression,in
R7939 T9518 T9514 punct .,implicated
R7940 T9519 T9524 advmod Moreover,recognize
R7941 T9520 T9524 punct ",",recognize
R7942 T9521 T9524 nsubj AP-1,recognize
R7943 T9522 T9521 cc and,AP-1
R7944 T9523 T9521 conj CREB,AP-1
R7945 T9524 T9524 ROOT recognize,recognize
R7946 T9525 T9529 det a,sequence
R7947 T9526 T9529 amod similar,sequence
R7948 T9527 T9529 compound DNA,sequence
R7949 T9528 T9529 amod binding,sequence
R7950 T9529 T9524 dobj sequence,recognize
R7951 T9530 T9524 prep in,recognize
R7952 T9531 T9533 det the,region
R7953 T9532 T9533 compound promoter,region
R7954 T9533 T9530 pobj region,in
R7957 T9536 T9537 compound TNF-α,gene35
R7958 T9537 T9534 pobj gene35,of
R7959 T9538 T9524 punct .,recognize
R7960 T9539 T9544 prep Given,is
R7961 T9540 T9541 det these,relationships
R7962 T9541 T9539 pobj relationships,Given
R7963 T9542 T9544 punct ",",is
R7964 T9543 T9544 nsubj it,is
R7965 T9544 T9544 ROOT is,is
R7966 T9545 T9544 acomp conceivable,is
R7967 T9546 T9549 mark that,exert
R7968 T9547 T9549 nsubj PTX,exert
R7969 T9548 T9549 aux may,exert
R7970 T9549 T9544 ccomp exert,is
R7971 T9550 T9549 dobj some,exert
R7972 T9551 T9550 prep of,some
R7973 T9552 T9554 poss its,actions
R7974 T9553 T9554 amod anti-inflammatory,actions
R7975 T9554 T9551 pobj actions,of
R7976 T9555 T9554 prep by,actions
R7977 T9556 T9555 pcomp affecting,by
R7978 T9557 T9558 amod AP-1,activation
R7979 T9558 T9556 dobj activation,affecting
R7980 T9559 T9556 cc and,affecting
R7981 T9560 T9556 conj favoring,affecting
R7982 T9561 T9560 dobj CREB,favoring
R7983 T9562 T9560 conj binding,favoring
R7984 T9563 T9549 punct ",",exert
R7985 T9564 T9565 advmod thus,inhibiting
R7986 T9565 T9549 advcl inhibiting,exert
R7987 T9566 T9568 amod TNF-α,transcription
R7988 T9567 T9568 compound gene,transcription
R7989 T9568 T9565 dobj transcription,inhibiting
R7990 T9569 T9544 punct .,is
R7991 T9570 T9571 amod Additional,studies
R7992 T9571 T9572 nsubj studies,are
R7993 T9572 T9572 ROOT are,are
R7994 T9573 T9572 acomp necessary,are
R7995 T9574 T9575 aux to,elucidate
R7996 T9575 T9573 xcomp elucidate,necessary
R7997 T9576 T9578 det the,interactions
R7998 T9577 T9578 amod complex,interactions
R7999 T9578 T9575 dobj interactions,elucidate
R8000 T9579 T9578 prep between,interactions
R8001 T9580 T9579 pobj CREB,between
R8002 T9581 T9580 punct ",",CREB
R8003 T9582 T9580 conj NF-κB,CREB
R8004 T9583 T9572 punct ",",are
R8005 T9584 T9572 cc and,are
R8006 T9585 T9572 conj AP-1,are
R8007 T9586 T9585 acl following,AP-1
R8008 T9587 T9588 compound LPS,exposure
R8009 T9588 T9586 pobj exposure,following
R8010 T9589 T9588 cc and,exposure
R8011 T9590 T9591 compound PDE,inhibition
R8012 T9591 T9588 conj inhibition,exposure
R8013 T9592 T9572 punct .,are
R8014 T9593 T9609 advmod Furthermore,explored
R8015 T9594 T9609 punct ",",explored
R8016 T9595 T9596 det the,effect
R8017 T9596 T9609 nsubjpass effect,explored
R8018 T9597 T9596 prep of,effect
R8019 T9598 T9597 pobj PTX,of
R8020 T9599 T9596 prep on,effect
R8021 T9600 T9602 det the,pathway
R8022 T9601 T9602 compound cGMP,pathway
R8023 T9602 T9599 pobj pathway,on
R8024 T9603 T9609 mark after,explored
R8025 T9604 T9605 compound LPS,stimulation
R8026 T9605 T9609 nsubjpass stimulation,explored
R8027 T9606 T9609 aux has,explored
R8028 T9607 T9609 neg not,explored
R8029 T9608 T9609 auxpass been,explored
R8030 T9609 T9609 ROOT explored,explored
R8031 T9610 T9609 punct ",",explored
R8032 T9611 T9613 mark although,plan
R8033 T9612 T9613 nsubj we,plan
R8034 T9613 T9609 advcl plan,explored
R8035 T9614 T9615 aux to,do
R8036 T9615 T9613 xcomp do,plan
R8037 T9616 T9615 advmod so,do
R8038 T9617 T9615 prep in,do
R8039 T9618 T9619 det the,future
R8040 T9619 T9617 pobj future,in
R8041 T9620 T9609 punct .,explored
R8042 T9621 T9626 prep In,demonstrated
R8043 T9622 T9621 pobj summary,In
R8044 T9623 T9626 punct ",",demonstrated
R8045 T9624 T9626 nsubj we,demonstrated
R8046 T9625 T9626 aux have,demonstrated
R8047 T9626 T9626 ROOT demonstrated,demonstrated
R8048 T9627 T9634 mark that,downregulates
R8049 T9628 T9629 compound PDE,inhibition
R8050 T9629 T9634 nsubj inhibition,downregulates
R8051 T9630 T9629 prep of,inhibition
R8052 T9631 T9633 amod human,cells
R8053 T9632 T9633 compound mononuclear,cells
R8054 T9633 T9630 pobj cells,of
R8055 T9634 T9626 ccomp downregulates,demonstrated
R8056 T9635 T9636 compound TNF-α,production
R8057 T9636 T9634 dobj production,downregulates
R8058 T9637 T9643 punct ",",through
R8059 T9638 T9639 advmod at,least
R8060 T9639 T9640 advmod least,in
R8061 T9640 T9643 prep in,through
R8062 T9641 T9640 pobj part,in
R8063 T9642 T9643 punct ",",through
R8064 T9643 T9634 prep through,downregulates
R8065 T9644 T9646 det a,mechanism
R8066 T9645 T9646 amod PKA-independent,mechanism
R8067 T9646 T9643 pobj mechanism,through
R8068 T9647 T9626 punct .,demonstrated
R8069 T9648 T9652 prep In,attenuates
R8070 T9649 T9648 pobj addition,In
R8071 T9650 T9652 punct ",",attenuates
R8072 T9651 T9652 nsubj PTX,attenuates
R8073 T9652 T9652 ROOT attenuates,attenuates
R8074 T9653 T9654 det the,activity
R8075 T9654 T9652 dobj activity,attenuates
R8076 T9655 T9654 prep of,activity
R8077 T9656 T9655 pobj NF-B,of
R8078 T9657 T9658 mark while,upregulating
R8079 T9658 T9652 advcl upregulating,attenuates
R8080 T9659 T9660 compound CREB,activation
R8081 T9660 T9658 dobj activation,upregulating
R8082 T9661 T9658 prep after,upregulating
R8083 T9662 T9663 compound LPS,stimulation
R8084 T9663 T9661 pobj stimulation,after
R8085 T9664 T9652 punct ",",attenuates
R8086 T9665 T9667 nsubj which,result
R8087 T9666 T9667 aux may,result
R8088 T9667 T9652 advcl result,attenuates
R8089 T9668 T9667 prep in,result
R8090 T9669 T9668 pobj modulation,in
R8091 T9670 T9669 prep of,modulation
R8092 T9671 T9673 amod pro-inflammatory,synthesis
R8093 T9672 T9673 compound mediator,synthesis
R8094 T9673 T9670 pobj synthesis,of
R8095 T9674 T9652 punct .,attenuates
R8096 T9675 T9679 advmod Therefore,serve
R8097 T9676 T9679 punct ",",serve
R8098 T9677 T9679 nsubj PTX,serve
R8099 T9678 T9679 aux may,serve
R8100 T9679 T9679 ROOT serve,serve
R8101 T9680 T9679 prep as,serve
R8102 T9681 T9684 det a,therapeutic
R8103 T9682 T9684 amod potential,therapeutic
R8104 T9683 T9684 compound adjunct,therapeutic
R8105 T9684 T9680 pobj therapeutic,as
R8106 T9685 T9684 prep for,therapeutic
R8107 T9686 T9687 det the,treatment
R8108 T9687 T9685 pobj treatment,for
R8109 T9688 T9687 prep of,treatment
R8110 T9689 T9688 pobj conditions,of
R8111 T9690 T9694 prep in,plays
R8112 T9691 T9690 pobj which,in
R8113 T9692 T9693 compound TNF-α,production
R8114 T9693 T9694 nsubj production,plays
R8115 T9694 T9687 relcl plays,treatment
R8116 T9695 T9697 det a,role
R8117 T9696 T9697 amod significant,role
R8118 T9697 T9694 dobj role,plays
R8119 T9698 T9679 punct .,serve
R100 T202 T200 pobj sepsis,in
R101 T203 T202 cc and,sepsis
R102 T204 T202 conj inflammation,sepsis
R339 T441 T443 det a,tool
R340 T442 T443 amod useful,tool
R341 T443 T440 pobj tool,as
R342 T444 T443 prep in,tool
R343 T445 T446 det the,attenuation
R344 T446 T444 pobj attenuation,in
R345 T447 T446 prep of,attenuation
R346 T448 T449 amod LPS-induced,inflammation
R347 T449 T447 pobj inflammation,of
R925 T1239 T1223 conj is,shown
R926 T1240 T1243 det a,cytokine
R927 T1241 T1243 amod potent,cytokine
R928 T1242 T1243 amod pro-inflammatory,cytokine
R929 T1243 T1239 attr cytokine,is
R930 T1244 T1243 acl involved,cytokine
R931 T1245 T1244 prep in,involved
R932 T1246 T1248 det a,spectrum
R933 T1247 T1248 amod wide,spectrum
R934 T1248 T1245 pobj spectrum,in
R935 T1249 T1248 prep of,spectrum
R936 T1250 T1251 amod cellular,responses
R937 T1251 T1249 pobj responses,of
R1095 T1409 T1406 attr body,is
R1096 T1410 T1409 prep of,body
R1097 T1411 T1410 pobj evidence,of
R1098 T1412 T1411 acl suggesting,evidence
R1099 T1413 T1416 mark that,function
R2160 T2733 T2732 pobj Pierce,from
R7955 T9534 T9533 prep of,region
R7956 T9535 T9537 det the,gene35
R60 T162 T159 pobj inhibition,of
R61 T163 T162 prep on,inhibition
R62 T164 T165 compound transcription,factor
R63 T165 T166 compound factor,activation
R64 T166 T163 pobj activation,on
R65 T167 T166 cc and,activation
R66 T168 T169 compound tumor,necrosis
R67 T169 T170 compound necrosis,factor-alpha
R68 T170 T166 conj factor-alpha,activation
R69 T171 T172 punct (,TNF-α
R70 T172 T170 appos TNF-α,factor-alpha
R71 T173 T170 punct ),factor-alpha
R72 T174 T166 conj production,activation
R73 T175 T174 prep in,production
R74 T176 T175 pobj lipopolysaccharide,in
R75 T177 T178 punct (,LPS
R76 T178 T176 appos LPS,lipopolysaccharide
R77 T179 T176 punct ),lipopolysaccharide
R755 T130 T130 ROOT Insights,Insights
R756 T131 T130 prep into,Insights
R757 T132 T133 det the,Regulation
R758 T133 T131 pobj Regulation,into
R759 T134 T133 prep of,Regulation
R760 T135 T136 compound TNF-α,Production
R761 T136 T134 pobj Production,of
R762 T137 T136 prep in,Production
R763 T138 T139 compound Human,Mononuclear
R764 T139 T140 compound Mononuclear,Cells

UBERON-AE

Id Subject Object Predicate Lexical cue
T1125 2469-2482 http://purl.obolibrary.org/obo/UBERON_0002405 denotes immune system
T2438 5131-5136 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T2439 5252-5257 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tubes
T2440 6695-6699 http://purl.obolibrary.org/obo/UBERON_0012245 denotes Silk
T3244 6741-6746 http://purl.obolibrary.org/obo/UBERON_0000178 denotes Blood
T3245 6848-6853 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T3246 7121-7126 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tubes
T3247 7243-7248 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T4128 9701-9705 http://purl.obolibrary.org/obo/UBERON_0001913 denotes milk
T4129 10592-10595 http://purl.obolibrary.org/obo/UBERON_0001460 denotes arm
T4130 10751-10755 http://purl.obolibrary.org/obo/UBERON_0001460 denotes arms
T8273 17496-17501 http://purl.obolibrary.org/obo/UBERON_0000062 denotes organ
T8274 19257-19262 http://purl.obolibrary.org/obo/UBERON_0000062 denotes organ
T8275 19595-19600 http://purl.obolibrary.org/obo/UBERON_0000062 denotes organ

GO-BP

Id Subject Object Predicate Lexical cue
T824 18-28 http://purl.obolibrary.org/obo/GO_0065007 denotes Regulation
T825 18-48 http://purl.obolibrary.org/obo/GO_0032680 denotes Regulation of TNF-α Production
T826 323-340 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α) production
T827 423-442 http://purl.obolibrary.org/obo/GO_0032680 denotes production of TNF-α
T828 590-606 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T829 18-37 http://purl.obolibrary.org/obo/GO_0042534 denotes Regulation of TNF-α
T830 18-37 http://purl.obolibrary.org/obo/GO_1904467 denotes Regulation of TNF-α
T831 18-48 http://purl.obolibrary.org/obo/GO_0032720 denotes Regulation of TNF-α Production
T832 18-48 http://purl.obolibrary.org/obo/GO_0032760 denotes Regulation of TNF-α Production
T833 32-48 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α Production
T834 323-340 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α) production
T835 423-442 http://purl.obolibrary.org/obo/GO_0032640 denotes production of TNF-α
T836 423-440 http://purl.obolibrary.org/obo/GO_0032640 denotes production of TNF
T837 105-122 http://purl.obolibrary.org/obo/GO_0008081 denotes Phosphodiesterase
T838 226-243 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T839 554-571 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T840 790-807 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T841 1672-1689 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T842 1925-1942 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T843 244-278 http://purl.obolibrary.org/obo/GO_1901484 denotes inhibition on transcription factor
T844 244-278 http://purl.obolibrary.org/obo/GO_0043433 denotes inhibition on transcription factor
T845 244-278 http://purl.obolibrary.org/obo/GO_0032792 denotes inhibition on transcription factor
T846 258-271 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T847 1806-1821 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T848 258-289 http://purl.obolibrary.org/obo/GO_0051091 denotes transcription factor activation
T849 258-289 http://purl.obolibrary.org/obo/GO_0032793 denotes transcription factor activation
T850 1787-1805 http://purl.obolibrary.org/obo/GO_0032793 denotes activation of CREB
T851 258-289 http://purl.obolibrary.org/obo/GO_1901485 denotes transcription factor activation
T852 300-308 http://purl.obolibrary.org/obo/GO_0001906 denotes necrosis
T853 300-308 http://purl.obolibrary.org/obo/GO_0008219 denotes necrosis
T854 300-308 http://purl.obolibrary.org/obo/GO_0019835 denotes necrosis
T855 300-308 http://purl.obolibrary.org/obo/GO_0070265 denotes necrosis
T856 517-529 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T857 2083-2095 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T858 790-817 http://purl.obolibrary.org/obo/GO_0051344 denotes phosphodiesterase inhibitor
T859 916-929 http://purl.obolibrary.org/obo/GO_0051591 denotes cAMP-response
T860 1305-1320 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T861 1334-1349 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T862 1429-1444 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T863 1773-1797 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB and the activation
T2109 2195-2210 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T2110 3309-3324 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T2111 2195-2225 http://purl.obolibrary.org/obo/GO_0042327 denotes phosphorylation and activation
T2112 2243-2272 http://purl.obolibrary.org/obo/GO_0035556 denotes intracellular protein kinases
T2113 2277-2290 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T2114 2373-2386 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T2115 2499-2512 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T2116 3332-3345 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T2117 3479-3492 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T2118 3843-3856 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T2119 4072-4085 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T2120 4342-4357 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T2121 2300-2331 http://purl.obolibrary.org/obo/GO_0051091 denotes Transcription factor activation
T2122 2499-2530 http://purl.obolibrary.org/obo/GO_0051091 denotes transcription factor activation
T2123 3825-3863 http://purl.obolibrary.org/obo/GO_0051091 denotes activation of the transcription factor
T2124 2300-2331 http://purl.obolibrary.org/obo/GO_0032793 denotes Transcription factor activation
T2125 2499-2530 http://purl.obolibrary.org/obo/GO_0032793 denotes transcription factor activation
T2126 3825-3863 http://purl.obolibrary.org/obo/GO_0032793 denotes activation of the transcription factor
T2127 4378-4393 http://purl.obolibrary.org/obo/GO_0032793 denotes CREB activation
T2128 4760-4775 http://purl.obolibrary.org/obo/GO_0032793 denotes CREB activation
T2129 2300-2331 http://purl.obolibrary.org/obo/GO_1901485 denotes Transcription factor activation
T2130 2499-2530 http://purl.obolibrary.org/obo/GO_1901485 denotes transcription factor activation
T2131 3825-3863 http://purl.obolibrary.org/obo/GO_1901485 denotes activation of the transcription factor
T2132 2391-2408 http://purl.obolibrary.org/obo/GO_0006412 denotes protein synthesis
T2133 2399-2408 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T2134 2730-2739 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T2135 2577-2585 http://purl.obolibrary.org/obo/GO_0001906 denotes necrosis
T2136 2577-2585 http://purl.obolibrary.org/obo/GO_0008219 denotes necrosis
T2137 2577-2585 http://purl.obolibrary.org/obo/GO_0019835 denotes necrosis
T2138 2577-2585 http://purl.obolibrary.org/obo/GO_0070265 denotes necrosis
T2139 2596-2613 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α) production
T2140 2982-2998 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T2141 4211-4227 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T2142 4902-4918 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T2143 2596-2613 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α) production
T2144 2982-2998 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T2145 4211-4227 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T2146 4902-4918 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T2147 2691-2699 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T2148 3664-3672 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T2149 2785-2802 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T2150 2804-2807 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T2151 2919-2922 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T2152 3099-3102 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T2153 4181-4184 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T2154 4669-4672 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T2155 2886-2895 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T2156 3415-3422 http://purl.obolibrary.org/obo/GO_0023052 denotes signals
T2157 2964-2987 http://purl.obolibrary.org/obo/GO_1904468 denotes downregulation of TNF-α
T2158 3237-3265 http://purl.obolibrary.org/obo/GO_0034199 denotes activation of Protein kinase
T2159 3237-3265 http://purl.obolibrary.org/obo/GO_0032147 denotes activation of Protein kinase
T2160 3237-3265 http://purl.obolibrary.org/obo/GO_0030295 denotes activation of Protein kinase
T2161 3269-3272 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T2162 3276-3279 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T2163 3767-3770 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T2164 4575-4578 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T2165 4883-4886 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T2166 3353-3366 http://purl.obolibrary.org/obo/GO_0051591 denotes cAMP-response
T2167 4026-4039 http://purl.obolibrary.org/obo/GO_0032088 denotes inhibit NF-κB
T2168 4026-4039 http://purl.obolibrary.org/obo/GO_0007252 denotes inhibit NF-κB
T3127 5219-5221 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3128 5413-5415 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3129 5647-5649 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3130 6217-6219 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3131 5516-5519 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T3884 8225-8241 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T3885 8417-8447 http://purl.obolibrary.org/obo/GO_0032680 denotes regulation in TNF-α production
T3886 8225-8241 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T3887 8431-8447 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T3888 8397-8407 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T3889 8417-8427 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T3890 8417-8436 http://purl.obolibrary.org/obo/GO_0042534 denotes regulation in TNF-α
T3891 8417-8436 http://purl.obolibrary.org/obo/GO_1904467 denotes regulation in TNF-α
T3892 8417-8447 http://purl.obolibrary.org/obo/GO_0032720 denotes regulation in TNF-α production
T3893 8417-8447 http://purl.obolibrary.org/obo/GO_0032760 denotes regulation in TNF-α production
T4080 8534-8550 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T4081 8534-8550 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T5960 12536-12539 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T5961 12613-12616 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T5962 12779-12782 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T5963 13086-13089 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T5964 13255-13258 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T5965 12572-12588 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T5966 12620-12636 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T5967 12712-12728 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T5968 12572-12588 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T5969 12620-12636 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T5970 12712-12728 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T6351 13889-13905 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T6352 13953-13969 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T6353 14100-14116 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T6354 13889-13905 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T6355 13953-13969 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T6356 14100-14116 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T6357 13935-13938 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T6358 13939-13958 http://purl.obolibrary.org/obo/GO_1904468 denotes inhibition on TNF-α
T6892 14188-14203 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T6893 14365-14380 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T6894 14554-14569 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T6895 14631-14646 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T6896 14774-14789 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T6897 14963-14978 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T6898 15212-15227 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T6899 14232-14243 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T6900 15124-15153 http://purl.obolibrary.org/obo/GO_0043392 denotes downregulation of DNA binding
T6901 15124-15153 http://purl.obolibrary.org/obo/GO_1904743 denotes downregulation of DNA binding
T6902 15206-15227 http://purl.obolibrary.org/obo/GO_0007252 denotes NF-κB phosphorylation
T7486 15263-15278 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T7487 15443-15458 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T7488 15540-15555 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T7489 15633-15648 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T7490 15772-15787 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T7491 16024-16039 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T7492 15263-15293 http://purl.obolibrary.org/obo/GO_0042327 denotes phosphorylation and activation
T7493 15304-15331 http://purl.obolibrary.org/obo/GO_0042327 denotes stimulation Phosphorylation
T7494 15373-15388 http://purl.obolibrary.org/obo/GO_0032793 denotes CREB activation
T7910 16194-16210 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T7911 16294-16310 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T7912 16734-16750 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T7913 16867-16883 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T7914 16194-16210 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T7915 16294-16310 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T7916 16734-16750 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T7917 16867-16883 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T7918 16230-16233 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T7919 16257-16260 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T7920 16402-16405 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T7921 16949-16952 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T7922 16276-16299 http://purl.obolibrary.org/obo/GO_1904468 denotes downregulation of TNF-α
T7923 16849-16872 http://purl.obolibrary.org/obo/GO_1904468 denotes downregulation of TNF-α
T9856 17080-17095 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T9857 19869-19884 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T9858 20765-20780 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T9859 21908-21923 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T9860 22927-22942 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T9861 23329-23342 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T9862 23741-23754 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T9863 24276-24289 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T9864 17335-17344 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T9865 17444-17453 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T9866 19322-19331 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T9867 20834-20843 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T9868 24906-24915 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T9869 17462-17473 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T9870 17630-17651 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammatory response
T9871 18594-18606 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T9872 19075-19087 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T9873 17744-17747 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T9874 22884-22887 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T9875 24419-24422 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T9876 24610-24613 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T9877 17791-17807 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T9878 18311-18327 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T9879 18789-18805 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T9880 22455-22471 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T9881 24666-24682 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T9882 25018-25034 http://purl.obolibrary.org/obo/GO_0032680 denotes TNF-α production
T9883 17791-17807 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T9884 18311-18327 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T9885 18789-18805 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T9886 22455-22471 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T9887 24666-24682 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T9888 25018-25034 http://purl.obolibrary.org/obo/GO_0032640 denotes TNF-α production
T9889 17973-17990 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T9890 18241-18258 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T9891 18931-18948 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T9892 17973-18001 http://purl.obolibrary.org/obo/GO_0051344 denotes phosphodiesterase inhibitors
T9893 18241-18268 http://purl.obolibrary.org/obo/GO_0051344 denotes phosphodiesterase inhibitor
T9894 18931-18958 http://purl.obolibrary.org/obo/GO_0051344 denotes phosphodiesterase inhibitor
T9895 18496-18499 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T9896 18669-18672 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T9897 18893-18896 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T9898 22134-22137 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T9899 22393-22396 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T9900 22509-22512 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T9901 22649-22652 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T9902 22762-22765 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T9903 24712-24715 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T9904 19499-19520 http://purl.obolibrary.org/obo/GO_0042119 denotes neutrophil activation
T9905 19826-19840 http://purl.obolibrary.org/obo/GO_0038061 denotes cascade. NF-κB
T9906 19826-19840 http://purl.obolibrary.org/obo/GO_0007249 denotes cascade. NF-κB
T9907 19909-19919 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T9908 19951-19967 http://purl.obolibrary.org/obo/GO_0006955 denotes immune responses
T9909 20171-20179 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T9910 20268-20286 http://purl.obolibrary.org/obo/GO_1903721 denotes activates the I-κB
T9911 20295-20298 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T9912 20322-20325 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T9913 20322-20327 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK-1
T9914 20332-20337 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK-2
T9915 20387-20402 http://purl.obolibrary.org/obo/GO_0016310 denotes Phosphorylation
T9916 20935-20950 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T9917 20970-20985 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T9918 21130-21145 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T9919 20435-20446 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T9920 21629-21640 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T9921 20806-20821 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T9922 20826-20843 http://purl.obolibrary.org/obo/GO_0006412 denotes protein synthesis
T9923 21549-21565 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T9924 23084-23110 http://purl.obolibrary.org/obo/GO_0043388 denotes activation and DNA binding
T9925 23084-23110 http://purl.obolibrary.org/obo/GO_1904744 denotes activation and DNA binding
T9926 24807-24822 http://purl.obolibrary.org/obo/GO_0032793 denotes CREB activation

GO-MF

Id Subject Object Predicate Lexical cue
T864 105-122 http://purl.obolibrary.org/obo/GO_0008081 denotes Phosphodiesterase
T865 226-243 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T866 554-571 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T867 790-807 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T868 1672-1689 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T869 1925-1942 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T870 938-945 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T871 980-987 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T872 1363-1370 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T873 1453-1460 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T874 1826-1833 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T875 938-953 http://purl.obolibrary.org/obo/GO_0005515 denotes binding protein
T876 976-987 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T877 1359-1370 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T878 1449-1460 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T879 1822-1833 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T880 980-996 http://purl.obolibrary.org/obo/GO_0051059 denotes binding of NF-κB
T2192 2499-2530 http://purl.obolibrary.org/obo/GO_0033613 denotes transcription factor activation
T2193 3825-3863 http://purl.obolibrary.org/obo/GO_0033613 denotes activation of the transcription factor
T2194 2785-2802 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T2195 2804-2807 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T2196 2919-2922 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T2197 3099-3102 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T2198 4181-4184 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T2199 4669-4672 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T2200 3237-3265 http://purl.obolibrary.org/obo/GO_0030295 denotes activation of Protein kinase
T2201 3269-3272 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T2202 3276-3279 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T2203 3767-3770 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T2204 4575-4578 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T2205 4883-4886 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T2206 3375-3382 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T2207 4300-4307 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T2208 4472-4479 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T2209 3375-3390 http://purl.obolibrary.org/obo/GO_0005515 denotes binding protein
T2210 4472-4487 http://purl.obolibrary.org/obo/GO_0005515 denotes binding protein
T2211 4296-4307 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T2212 4467-4479 http://purl.obolibrary.org/obo/GO_0008140 denotes CREB binding
T2213 4489-4492 http://purl.obolibrary.org/obo/GO_0008140 denotes CBP
T3144 5219-5221 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3145 5413-5415 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3146 5647-5649 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3147 6217-6219 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3148 5516-5519 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T3149 5804-5814 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T4827 9850-9858 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T4828 9943-9951 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T4829 9983-9991 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T4830 10167-10175 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T4831 10363-10371 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T5578 11169-11182 http://purl.obolibrary.org/obo/GO_0051059 denotes NF-κB binding
T5579 11175-11182 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T5580 11771-11778 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T5581 11878-11885 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T5971 12536-12539 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T5972 12613-12616 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T5973 12779-12782 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T5974 13086-13089 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T5975 13255-13258 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T6369 13935-13938 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T6924 15014-15025 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T6925 15142-15153 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T6926 15018-15025 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T6927 15146-15153 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T7498 15964-15975 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T7499 16129-16140 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA-binding
T7500 15968-15975 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T7501 16133-16140 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T7944 16230-16233 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T7945 16257-16260 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T7946 16402-16405 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T7947 16949-16952 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T10029 17744-17747 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T10030 22884-22887 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T10031 24419-24422 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T10032 24610-24613 http://purl.obolibrary.org/obo/GO_0004114 denotes PDE
T10033 17973-17990 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T10034 18241-18258 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T10035 18931-18948 http://purl.obolibrary.org/obo/GO_0008081 denotes phosphodiesterase
T10036 18496-18499 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T10037 18669-18672 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T10038 18893-18896 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T10039 22134-22137 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T10040 22393-22396 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T10041 22509-22512 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T10042 22649-22652 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T10043 22762-22765 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T10044 24712-24715 http://purl.obolibrary.org/obo/GO_0004691 denotes PKA
T10045 20295-20298 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T10046 20322-20325 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T10047 20322-20327 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK-1
T10048 20332-20337 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK-2
T10049 21018-21029 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA-binding
T10050 21810-21821 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA-binding
T10051 23099-23110 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T10052 24026-24037 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T10053 21022-21029 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T10054 21814-21821 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T10055 22980-22987 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T10056 23103-23110 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T10057 24030-24037 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T10058 24240-24247 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T10059 22980-22995 http://purl.obolibrary.org/obo/GO_0008140 denotes binding of CREB
T10060 23503-23506 http://purl.obolibrary.org/obo/GO_0008140 denotes CBP
T10061 24026-24046 http://purl.obolibrary.org/obo/GO_0043565 denotes DNA binding sequence

GO-CC

Id Subject Object Predicate Lexical cue
T3153 5835-5839 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T3154 6132-6141 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T3155 6530-6539 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T3901 6759-6763 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T3902 6990-6994 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T3903 7370-7374 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T3904 7470-7475 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3905 7662-7667 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3906 7698-7703 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4086 8566-8571 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4087 8654-8659 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4832 8956-8960 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T4833 9392-9396 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T4834 9655-9664 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T4835 9670-9679 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T4836 10046-10055 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T4837 10290-10298 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T4838 9850-9858 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T4839 9943-9951 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T4840 9983-9991 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T4841 10167-10175 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T4842 10363-10371 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T4843 9850-9858 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T4844 9943-9951 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T4845 9983-9991 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T4846 10167-10175 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T4847 10363-10371 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T5582 12200-12208 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T5583 12459-12468 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T5978 12779-12782 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T5979 13086-13089 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T5980 13255-13258 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T5981 12567-12571 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T5982 12652-12657 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5983 12829-12834 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6928 15095-15100 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7502 16091-16096 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7948 16230-16233 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T7949 16257-16260 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T7950 16402-16405 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T7951 16949-16952 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T7952 16341-16346 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7953 16384-16389 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7954 16483-16488 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7955 16641-16646 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7956 16921-16926 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10062 17007-17012 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10063 18842-18847 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10064 19698-19703 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10065 21399-21404 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10066 21997-22002 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10067 22019-22024 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10068 24646-24651 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10069 17029-17042 http://purl.obolibrary.org/obo/GO_0005576 denotes extracellular
T10070 18413-18426 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T10071 21459-21472 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T10072 18496-18499 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T10073 18669-18672 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T10074 18893-18896 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T10075 22134-22137 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T10076 22393-22396 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T10077 22509-22512 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T10078 22649-22652 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T10079 22762-22765 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T10080 24712-24715 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T10081 20102-20111 http://purl.obolibrary.org/obo/GO_0005737 denotes cytoplasm
T10082 20295-20307 http://purl.obolibrary.org/obo/GO_0008385 denotes IKK) complex
T881 398-403 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T882 674-679 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T883 1122-1127 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2214 2126-2134 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T2215 2238-2242 http://purl.obolibrary.org/obo/GO_0018995 denotes host
T5976 12536-12539 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T5977 12613-12616 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T2216 2464-2468 http://purl.obolibrary.org/obo/GO_0018995 denotes host
T2217 2238-2256 http://purl.obolibrary.org/obo/GO_0043656 denotes host intracellular
T2218 2243-2256 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T2219 3129-3142 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T2220 3599-3612 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T2221 4002-4015 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T2222 2768-2773 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2223 3049-3054 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2224 4806-4811 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2225 3269-3272 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T2226 3276-3279 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T2227 3767-3770 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T2228 4575-4578 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T2229 4883-4886 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T2230 3432-3439 http://purl.obolibrary.org/obo/GO_0005634 denotes nucleus
T2231 3843-3871 http://purl.obolibrary.org/obo/GO_0044798 denotes transcription factor nuclear
T3150 5516-5519 http://purl.obolibrary.org/obo/GO_0005952 denotes PKA
T3151 5804-5814 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T3152 5804-5814 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies

sentences

Id Subject Object Predicate Lexical cue
T110 0-76 Sentence denotes Insights into the Regulation of TNF-α Production in Human Mononuclear Cells:
T111 77-133 Sentence denotes The Effects of Non-Specific Phosphodiesterase Inhibition
T112 145-154 Sentence denotes OBJECTIVE
T113 155-404 Sentence denotes The objective of this study was to determine the effect of nonspecific phosphodiesterase inhibition on transcription factor activation and tumor necrosis factor-alpha (TNF-α) production in lipopolysaccharide (LPS)-stimulated human mononuclear cells.
T114 406-418 Sentence denotes INTRODUCTION
T115 419-530 Sentence denotes The production of TNF-α following LPS stimulation is one of the key steps in bacterial sepsis and inflammation.
T116 531-646 Sentence denotes The mechanism by which phosphodiesterase inhibition alters TNF-α production in the presence of LPS remains unclear.
T117 648-655 Sentence denotes METHODS
T118 656-818 Sentence denotes Human mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor.
T119 819-975 Sentence denotes Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed.
T120 976-1059 Sentence denotes DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay.
T121 1060-1232 Sentence denotes TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA).
T122 1234-1241 Sentence denotes RESULTS
T123 1242-1389 Sentence denotes PTX was demonstrated to significantly reduce cytoplasmic I-κBα phosphorylation, nuclear p65 phosphorylation, and the DNA binding activity of NF-κB.
T124 1390-1478 Sentence denotes In contrast, PTX markedly enhanced the phosphorylation and DNA binding activity of CREB.
T125 1479-1613 Sentence denotes Cells concomitantly treated with PTX and LPS secreted similar levels of TNF-α in the presence and absence Protein kinase A inhibition.
T126 1615-1625 Sentence denotes DISCUSSION
T127 1626-1899 Sentence denotes The increased level of cAMP that results from phosphodiesterase inhibition affects cytoplasmic and nuclear events, resulting in the attenuation of NF-κB and the activation of CREB transcriptional DNA binding through pathways that are partially Protein kinase A-independent.
T128 1901-1911 Sentence denotes CONCLUSION
T129 1912-2096 Sentence denotes PTX-mediated phosphodiesterase inhibition occurs partially through a Protein kinase A-independent pathway and may serve as a useful tool in the attenuation of LPS-induced inflammation.
T1149 2112-2299 Sentence denotes The bacterial membrane component lipopolysaccharide (LPS) is capable of initiating phosphorylation and activation of multiple host intracellular protein kinases and transcription factors.
T1150 2300-2486 Sentence denotes Transcription factor activation, which results in the modulation of gene transcription and protein synthesis, is a critical element in the defense mechanism of the host immune system.1,2
T1151 2487-2710 Sentence denotes LPS-induced transcription factor activation has been shown to be a key regulator of tumor necrosis factor-α (TNF-α) production.3 TNF-α is a potent pro-inflammatory cytokine involved in a wide spectrum of cellular responses.
T1152 2711-3055 Sentence denotes Furthermore, TNF-α synthesis can be attenuated in immune cells exposed to phosphodiesterase (PDE) inhibition after challenge by a variety of pro-inflammatory stimulants.4 The signaling mechanisms affected by PDE inhibition, which ultimately lead to the downregulation of TNF-α production, have not been well characterized in inflammatory cells.
T1153 3056-3683 Sentence denotes Classically, it has been demonstrated that PDE inhibition results in the intracellular accumulation of the second messenger cyclic adenosine-3,5-monophosphate (cAMP) and subsequent activation of Protein kinase A (PKA).5 PKA activation then leads to the phosphorylation of the transcription factor cAMP-response element binding protein (CREB), transmission of signals into the nucleus, and the subsequent modulation of gene transcription.6 This apparently simple linear cascade does not fully explain the mechanism by which an elevation in the intracellular cAMP level exerts wide-ranging effects on multiple cellular functions.
T1154 3684-3812 Sentence denotes There is a growing body of evidence suggesting that cAMP may function through both PKA-dependent and -independent mechanisms.6–8
T1155 3813-3942 Sentence denotes LPS-induced activation of the transcription factor nuclear factor-κB (NF-κB) has also been the focus of a great deal of research.
T1156 3943-4119 Sentence denotes It has been clearly demonstrated that agents that increase intracellular cAMP also inhibit NF-κB-dependent pro-inflammatory gene transcription, particularly of the TNF-α gene.9
T1157 4120-4228 Sentence denotes Controversy exists regarding the exact mechanism(s) by which PDE inhibition down-regulates TNF-α production.
T1158 4229-4494 Sentence denotes Possibilities include, but are not limited to, inhibition of NF-κB DNA binding activity, downregulation of NF-κB transcriptional activity, increased CREB activation, and competition between NF-κB and CREB for common co-activators such as CREB binding protein (CBP).
T1159 4495-4579 Sentence denotes It is also not known whether these processes rely solely upon the activation of PKA.
T1160 4580-4812 Sentence denotes Therefore, the objective of the present study is to determine the effects of nonspecific PDE inhibition with 1-[5-oxohexyl]-3,7-dimethylxanthine (Pentoxifylline; PTX) on NF-κB and CREB activation in vitro in human mononuclear cells.
T1161 4813-4919 Sentence denotes With the use of specific inhibitors, we also investigated the role of PKA in LPS-induced TNF-α production.
T2453 172-5223 Sentence denotes this study was to determine the effect of nonspecific phosphodiesterase inhibition on transcription factor activation and tumor necrosis factor-alpha (TNF-α) production in lipopolysaccharide (LPS)-stimulated human mononuclear cells. INTRODUCTION The production of TNF-α following LPS stimulation is one of the key steps in bacterial sepsis and inflammation. The mechanism by which phosphodiesterase inhibition alters TNF-α production in the presence of LPS remains unclear. METHODS Human mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor. Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed. DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay. TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA). RESULTS PTX was demonstrated to significantly reduce cytoplasmic I-κBα phosphorylation, nuclear p65 phosphorylation, and the DNA binding activity of NF-κB. In contrast, PTX markedly enhanced the phosphorylation and DNA binding activity of CREB. Cells concomitantly treated with PTX and LPS secreted similar levels of TNF-α in the presence and absence Protein kinase A inhibition. DISCUSSION The increased level of cAMP that results from phosphodiesterase inhibition affects cytoplasmic and nuclear events, resulting in the attenuation of NF-κB and the activation of CREB transcriptional DNA binding through pathways that are partially Protein kinase A-independent. CONCLUSION PTX-mediated phosphodiesterase inhibition occurs partially through a Protein kinase A-independent pathway and may serve as a useful tool in the attenuation of LPS-induced inflammation. INTRODUCTION The bacterial membrane component lipopolysaccharide (LPS) is capable of initiating phosphorylation and activation of multiple host intracellular protein kinases and transcription factors. Transcription factor activation, which results in the modulation of gene transcription and protein synthesis, is a critical element in the defense mechanism of the host immune system.1,2 LPS-induced transcription factor activation has been shown to be a key regulator of tumor necrosis factor-α (TNF-α) production.3 TNF-α is a potent pro-inflammatory cytokine involved in a wide spectrum of cellular responses. Furthermore, TNF-α synthesis can be attenuated in immune cells exposed to phosphodiesterase (PDE) inhibition after challenge by a variety of pro-inflammatory stimulants.4 The signaling mechanisms affected by PDE inhibition, which ultimately lead to the downregulation of TNF-α production, have not been well characterized in inflammatory cells. Classically, it has been demonstrated that PDE inhibition results in the intracellular accumulation of the second messenger cyclic adenosine-3,5-monophosphate (cAMP) and subsequent activation of Protein kinase A (PKA).5 PKA activation then leads to the phosphorylation of the transcription factor cAMP-response element binding protein (CREB), transmission of signals into the nucleus, and the subsequent modulation of gene transcription.6 This apparently simple linear cascade does not fully explain the mechanism by which an elevation in the intracellular cAMP level exerts wide-ranging effects on multiple cellular functions. There is a growing body of evidence suggesting that cAMP may function through both PKA-dependent and -independent mechanisms.6–8 LPS-induced activation of the transcription factor nuclear factor-κB (NF-κB) has also been the focus of a great deal of research. It has been clearly demonstrated that agents that increase intracellular cAMP also inhibit NF-κB-dependent pro-inflammatory gene transcription, particularly of the TNF-α gene.9 Controversy exists regarding the exact mechanism(s) by which PDE inhibition down-regulates TNF-α production. Possibilities include, but are not limited to, inhibition of NF-κB DNA binding activity, downregulation of NF-κB transcriptional activity, increased CREB activation, and competition between NF-κB and CREB for common co-activators such as CREB binding protein (CBP). It is also not known whether these processes rely solely upon the activation of PKA. Therefore, the objective of the present study is to determine the effects of nonspecific PDE inhibition with 1-[5-oxohexyl]-3,7-dimethylxanthine (Pentoxifylline; PTX) on NF-κB and CREB activation in vitro in human mononuclear cells. With the use of specific inhibitors, we also investigated the role of PKA in LPS-induced TNF-α production. MATERIALS AND METHODS This study was approved by the Human Research Protections Program and the Institutional Review Board. Written consent to participate in the study was obtained from all volunteers prior to blood donation. Heparinized Vacutainers were purchased from Becton Dickinson (San Jose, CA).
T2454 5224-5312 Sentence denotes 1.5-mL Eppendorf centrifuge tubes were purchased from Fisher Scientific (Pittsburg, PA).
T2455 5313-5417 Sentence denotes Hank’s balanced salt solution (HBSS) and RPMI 1640 were obtained from Irvine Scientific (Santa Ana, CA).
T2456 5418-5511 Sentence denotes LPS from Escherichia coli serotype 0111:B4 and PTX were purchased from Sigma (St. Louis, MO).
T2457 5512-5651 Sentence denotes The PKA inhibitor, N-[2-((p-bromocinnamyl)amino)ethyl]-5-isoguinolinesulfonamide-2HCl] (H89), was purchased from Calbiochem (La Jolla, CA).
T2458 5652-5961 Sentence denotes Antibodies for phosphorylated CREB (serine 133), phosphorylated I-κBα (serines 32 and 36), phosphorylated NF-κB p65 subunit (serine 276), and secondary antibodies were purchased from Cell Signaling (Beverly, MA). Dextran T500 and Percoll were received from GE Healthcare/Amersham Biosciences (Piscataway, NJ).
T2459 5962-6221 Sentence denotes The enzyme-linked immunosorbent assay (ELISA) for TNF-α was obtained from Quantikine R&D Systems (Minneapolis, MN). Tris-glycine and DNA retardation gels, nitrocellulose membranes, and western blot running buffers were obtained from Invitrogen (Carlsbad, CA).
T2460 6222-6439 Sentence denotes NE-PER® nuclear and cytoplasmic extraction reagents, the BCA protein reagent kit, the Supersignal West Pico Chemiluminescent Kit, and the LightShift Chemiluminescent EMSA Kit were purchased from Pierce (Rockland, IL).
T2461 6440-6523 Sentence denotes CREB and NF-κB DNA oligonucleotide probes were synthesized by IDT (Coralville, IA).
T2462 6524-6602 Sentence denotes Nylon membranes were purchased from Roche Applied Sciences (Indianapolis, IN).
T2463 6603-6722 Sentence denotes Western blot band quantification was performed with the UN-SCAN-IT Gel Digitizing software (Silk Scientific, Orem, UT).
T3255 6724-6789 Sentence denotes Human Peripheral Blood Mononuclear Cell Isolation and Stimulation
T3256 6790-6887 Sentence denotes Human mononuclear cells were isolated from the peripheral blood of four healthy human volunteers.
T3257 6888-7093 Sentence denotes The sample size was chosen from our previously published work, which demonstrated reliable results.10 Cell isolation and all subsequent experiments were conducted under sterile and pyrogen-free conditions.
T3258 7094-7228 Sentence denotes Blood collected in heparin tubes was incubated with Dextran T500 and the red cells were sedimented for 40 minutes at room temperature.
T3259 7229-7369 Sentence denotes The resultant serum was then washed with HBSS and separated by Percoll gradient centrifugation according to the manufacturer’s instructions.
T3260 7370-7460 Sentence denotes Cell viability was assessed by trypan blue dye exclusion, with purity of greater than 95%.
T3261 7461-7585 Sentence denotes Isolated cells were resuspended in RPMI 1640 supplemented with 10% FBS and 5 mM HEPES at a concentration of 1 x 107cells/mL.
T3262 7586-7688 Sentence denotes Each subsequent experiment listed below was conducted on samples of 5 x 106 cells per treatment group.
T3263 7689-7876 Sentence denotes Isolated cells were stimulated with either HBSS as a negative control, LPS (1 μg/mL), PTX (20 mM), or concomitant LPS / PTX, at the above-mentioned concentrations, for 30 minutes at 37ºC.
T3264 7877-8001 Sentence denotes Cells were placed on ice for 10 minutes to stop the reaction and the samples were then stored at −70°C for further analysis.
T3265 8002-8242 Sentence denotes The stimulation times and concentrations of LPS and PTX utilized in this study were determined in previous pilot studies done in our laboratory, which examined the effects of increasing concentrations of PTX on LPS-induced TNF-α production.
T3266 8243-8503 Sentence denotes LPS at a concentration of 1 μg/mL and PTX at a concentration of 20 mM were the minimum concentrations necessary to produce a reproducible and reliable up-regulation and down-regulation in TNF-α production, respectively, in quantitative assays (data not shown).
T3968 8505-8571 Sentence denotes Effect of PTX on LPS-induced TNF-α production by mononuclear cells
T3969 8572-8773 Sentence denotes The concentration of TNF-α was quantitatively determined by ELISA in the media of cells exposed to the treatments described above after stimulation for up to a maximum of 18 hours, at 3-hour intervals.
T3970 8774-8809 Sentence denotes The results are expressed in pg/mL.
T4138 8811-8867 Sentence denotes SDS-PAGE and Immunoblotting (I-κBα, NF-κB p65, and CREB)
T4139 8868-8968 Sentence denotes Following stimulation, PBMCs were washed with ice-cold PBS and centrifuged to collect a cell pellet.
T4140 8969-9066 Sentence denotes The pellet was resuspended in ice-cold SDS sample buffer supplemented with 100 mM dithiothreitol.
T4141 9067-9256 Sentence denotes Cytoplasmic and nuclear extracts were isolated with NE-PER nuclear and cytoplasmic extraction reagents with 1x Halt Protease Inhibitor Cocktail according to the manufacturer’s instructions.
T4142 9257-9391 Sentence denotes Protein concentrations were determined with the BCA protein reagent kit for each sample according to a standardized curve for albumin.
T4143 9392-9479 Sentence denotes Cell lysates were collected by boiling the samples in a 100ºC water bath for 5 minutes.
T4144 9480-9665 Sentence denotes Ten μg of protein per sample was separated by SDS-polyacrylamide gel electrophoresis through 8–16% tris-glycine polyacrylamide gradient gels and transferred to nitrocellulose membranes.
T4145 9666-9787 Sentence denotes The membranes were blocked with 5% milk in Tris-buffered saline/Tween 20 (Fischer Scientific, Pittsburgh, PA) for 1 hour.
T4146 9788-10041 Sentence denotes Cytoplasmic extracts were incubated with phosphorylated I-κBα antibody (1:200), while nuclear extracts were incubated with either phosphorylated NF-κB p65 antibody (1:500) or phosphorylated CREB antibody (1:500) overnight at 4°C in separate experiments.
T4147 10042-10259 Sentence denotes The membranes were washed with Tris-buffered saline/Tween 20 and incubated for 1 hour at room temperature with the secondary antibody, horseradish peroxidase-linked anti-rabbit IgG diluted 1:2000 in blocking solution.
T4148 10260-10418 Sentence denotes After repeated washing of the membrane, the Supersignal West Pico Chemiluminescent Kit was applied for antibody detection per the manufacturer’s instructions.
T4149 10419-10485 Sentence denotes Western blot data is presented as a percentage of LPS stimulation.
T4150 10486-10682 Sentence denotes The percentage of LPS stimulation was calculated by dividing the mean band pixel total for each treatment arm divided by the mean pixel total of samples stimulated with LPS and multiplying by 100.
T4151 10683-10800 Sentence denotes Thus, LPS stimulation is reported as 100% and each of the treatment arms is reported as a percent of LPS stimulation.
T4895 10802-10860 Sentence denotes NF-κB and CREB Electrophoretic Mobility Shift Assay (EMSA)
T4896 10861-10962 Sentence denotes The non-radioactive LightShift Chemiluminescent EMSA Kit was used to detect DNA-protein interactions.
T4897 10963-11202 Sentence denotes The NF-κB 3′ biotin end-labeled DNA oligonucleotide used as a probe for the EMSA was a 42-bp double stranded construct (5′-TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC – 3′) containing two tandemly repeated NF-κB binding sites (underlined).
T4898 11203-11342 Sentence denotes Specificity was determined by a competition assay with the addition of 200 molar excess of unlabeled double stranded NF-κB oligonucleotide.
T4899 11343-11550 Sentence denotes The CREB 3′ biotin end-labeled DNA oligonucleotide used as a probe for the EMSA was a 23-bp double stranded construct (5′-TTT TCG AGC TCTGACGTCAGA-GC – 3′) containing the CRE consensus sequence (underlined).
T4900 11551-11689 Sentence denotes Specificity was determined by a competition assay with the addition of 200 molar excess of unlabeled double stranded CREB oligonucleotide.
T4901 11690-11767 Sentence denotes Nuclear extracts (10 μg) were incubated with 5 nM NF-κB or CREB probe (NF-kB:
T4902 11768-11874 Sentence denotes 1x binding buffer, 50 mM KCl, 1 mM EDTA, 1 mM DTT, 0.1% NP40, 10% glycerol, and 50 ng/μl poly dI-dC, CREB:
T4903 11875-12096 Sentence denotes 1x binding buffer, 20 mM Tris, pH 7.5, 50 mM KCl, 1 mM EDTA, 1 mM DTT, 0.10% NP40, 6% glycerol, 0.1 mg/mL BSA and 50 ng/μl poly dI-dC) and were then electrophoresed through a 6% DNA retardation gel at 100V for 90 minutes.
T4904 12097-12308 Sentence denotes The gels were electrophoretically transferred at 380mA for 1 hour on ice to a positively charged nylon membrane and immediately cross-linked for 15 minutes with a UV transilluminator equipped with a 312 nm bulb.
T4905 12309-12448 Sentence denotes Streptavidin-horseradish peroxidase conjugate and the LightShift Chemiluminescent Substrate were used to detect the biotin end-labeled DNA.
T4906 12449-12526 Sentence denotes The nylon membranes were exposed to x-ray film for 1–3 minutes for detection.
T5643 12528-12588 Sentence denotes Role of PKA on LPS-induced mononuclear cell TNF-α production
T5644 12589-12807 Sentence denotes To evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized.
T5645 12808-12980 Sentence denotes Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC.
T5646 12981-13299 Sentence denotes The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments.
T5647 13300-13380 Sentence denotes The TNF-α concentration in the supernatant was measured quantitatively by ELISA.
T5648 13381-13416 Sentence denotes The results are expressed in pg/mL.
T6066 13418-13438 Sentence denotes Statistical Analysis
T6067 13439-13561 Sentence denotes The experimental results obtained in this study were derived from four separate experiments with healthy volunteer donors.
T6068 13562-13632 Sentence denotes Each assay was performed in duplicate or triplicate where appropriate.
T6069 13633-13669 Sentence denotes Data is presented as the mean ± SEM.
T6070 13670-13794 Sentence denotes Statistical differences between groups were determined by one-way analysis of variance (ANOVA) with a Bonferroni correction.
T6071 13795-13844 Sentence denotes Statistical significance was defined as P < 0.05.
T6239 13855-13905 Sentence denotes The effects of PTX on LPS-induced TNF-α production
T6240 13906-14028 Sentence denotes To determine the duration of PDE inhibition on TNF-α production after LPS stimulation, time course studies were conducted.
T6241 14029-14137 Sentence denotes In accordance with prior work, PTX had a rapid and sustained effect on TNF-α production in vitro (Figure 1).
T6497 14139-14181 Sentence denotes PTX decreases LPS-induced NF-κB activation
T6498 14182-14346 Sentence denotes Since phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα.
T6499 14347-14487 Sentence denotes Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P < 0.05).
T6500 14488-14590 Sentence denotes The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)
T6501 14591-14719 Sentence denotes In a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P < 0.01).
T6502 14720-14887 Sentence denotes PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).
T6503 14888-15044 Sentence denotes EMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB.
T6504 15045-15240 Sentence denotes The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B).
T7083 15242-15315 Sentence denotes PTX upregulates CREB phosphorylation and activation after LPS stimulation
T7084 15316-15389 Sentence denotes Phosphorylation of nuclear CREB was used as a marker for CREB activation.
T7085 15390-15496 Sentence denotes LPS stimulation caused a negligible increase in CREB phosphorylation when compared to control (Figure 4A).
T7086 15497-15576 Sentence denotes PTX alone caused a marked increase in CREB phosphorylation (P < 0.01 vs. HBSS).
T7087 15577-15739 Sentence denotes When LPS and PTX exposure occurred simultaneously, CREB phosphorylation was significantly higher than with LPS stimulation alone (543 ± 92 vs. 100 ± 0; P < 0.01).
T7088 15740-15942 Sentence denotes In addition, the amount of CREB phosphorylation seen with concomitant LPS and PTX treatment was less than that seen with PTX alone, although this difference was not statistically significant (P = 0.08).
T7089 15943-16063 Sentence denotes To determine if CREB-DNA binding was affected by PTX in a manner similar to CREB phosphorylation, an EMSA was performed.
T7090 16064-16175 Sentence denotes Exposure of LPS-stimulated cells to PTX similarly increased CREB DNA-binding compared to LPS alone (Figure 4B).
T7606 16177-16244 Sentence denotes Effect of PTX on TNF-α production in the presence of PKA inhibition
T7607 16245-16447 Sentence denotes The role of PKA in PTX-induced downregulation of TNF-α production in LPS-stimulated mononuclear cells was assessed by treating mononuclear cells with H89, a PKA inhibitor, prior to LPS and PTX exposure.
T7608 16448-16622 Sentence denotes The addition PTX to LPS-stimulated cells resulted in a reduction in TNF-α, similar to that observed in our previous experiments (529 pg/mL ± 112 vs. 37 pg/mL ± 10; P < 0.01).
T7609 16623-16821 Sentence denotes Pre-incubation of cells with H89 prior to LPS and PTX exposure did not result in any significant modulation of TNF-α production when compared to that demonstrated with PTX alone (P = 0.2; Figure 5).
T7610 16822-16965 Sentence denotes These results suggest that downregulation of TNF-α production by PTX in LPS-stimulated mononuclear cells is, at least in part, PKA-independent.
T8375 16978-17564 Sentence denotes The exposure of inflammatory cells to a variety of extracellular stimuli results in the initiation of transcriptional and posttranscriptional events, which culminate in the production of pro-inflammatory mediators.11–14 TNF-α is released very early following shock and is considered an important mediator of the inflammatory cascade, since it regulates the synthesis of several other critical cytokines and chemokines.15 A clear relationship between increased TNF-α synthesis and the development of shock and multiple organ dysfunction has been observed in animal models of sepsis.15–17
T8376 17565-17743 Sentence denotes Because of its importance in orchestrating multiple steps of the inflammatory response, strategies aimed at decreasing TNF-α levels or attenuating its production seem attractive.
T8377 17744-17843 Sentence denotes PDE inhibitors have been shown to downregulate TNF-α production in distinct human cell populations.
T8378 17844-18196 Sentence denotes We have previously shown that, in general, TNF-α levels continue to increase after LPS exposure and that concomitant infusion of phosphodiesterase inhibitors can markedly decrease TNF-α levels.18–19 In this in vitro study, we verified the sustained attenuation of LPS-induced TNF-α expression over time in an isolated human mononuclear cell population.
T8379 18197-18364 Sentence denotes The mechanisms by which PTX, a non-specific phosphodiesterase inhibitor, significantly and consistently decreases TNF-α production have not been completely elucidated.
T8380 18365-18500 Sentence denotes It has been postulated that drugs that increase intracellular cAMP exert their anti-inflammatory effects through the activation of PKA.
T8381 18501-18908 Sentence denotes This classic pathway has been challenged as the sole mechanism involved in the modulation of inflammation by cAMP-enhancing drugs, and alternative mechanisms involving PKA-independent pathways have been proposed.6–8 In this series of experiments, we observed a significant attenuation of TNF-α production in LPS-stimulated human mononuclear cells following PTX administration, independent of PKA activation.
T8382 18909-19332 Sentence denotes We used a nonspecific phosphodiesterase inhibitor, PTX, in the present study because it has been used clinically in the treatment of a variety of conditions in which inflammation is an important component of the pathophysiology of the disease process.19 Additionally, the use of PTX in sepsis as an adjunct to other treatments to maintain adequate organ function has been explored based upon its effects on TNF-α synthesis.
T8383 19333-19834 Sentence denotes However, the reported beneficial effects of PTX are not only related to the downregulation of TNF-αbut also to PTX’s hemorrheologic properties, its ability to reduce neutrophil activation, and its beneficial effects on microcirculation, cardiac performance, and organ injury.20–25 Herein, we demonstrated that the downregulatory effects of PTX in human mononuclear cells involves modulation of pathways that involve NF-κB and CREB, two major transcription factors involved in the inflammatory cascade.
T8384 19835-19998 Sentence denotes NF-κB/Rel constitutes a family of transcriptional factors involved in the regulation of numerous cytokine genes and immune responses in different cell populations.
T8385 19999-20160 Sentence denotes The most abundant form of NF-κB is the p50–p65 heterodimer.26 The inactive form of NF-κB exists in the cytoplasm bound to an inhibitory complex containing I-κBα.
T8386 20161-20386 Sentence denotes Following cellular stimulation with LPS and chemotactic factors, NF-κB inducible kinase phosphorylates and activates the I-κB kinase (IKK) complex consisting of IKK-1 and IKK-2, which in turn phosphorylates the I-κBα subunit.
T8387 20387-20495 Sentence denotes Phosphorylation, ubiquitination, and subsequent degradation of I-κBα results in NF-κB nuclear translocation.
T8388 20496-20849 Sentence denotes This allows the transcription factor to bind to promoter regions of specific pro-inflammatory genes and influence transcription.27,28 Because a number of potential κB sites are present in the nucleotide sequences of the TNF-α gene, NF-κB is considered to be a critical transcriptional factor involved in TNF-α gene expression and protein synthesis.29,30
T8389 20850-21146 Sentence denotes In this set of experiments, we demonstrated that PTX downregulates cytoplasmic I-κBα phosphorylation, nuclear NF-κB p65 phosphorylation/translocation, as well as NF-κB DNA-binding after LPS stimulation, suggesting that PTX exerts its function proximal to or at the level of I-κBα phosphorylation.
T8390 21147-21273 Sentence denotes Previous studies that have evaluated the effects of cAMP-elevating drugs on I-κBα and NF-κB have reported conflicting results.
T8391 21274-21745 Sentence denotes Haddad et al. reported results similar to ours with respect to the effects of PTX on I-κBα and NF-κB in pulmonary epithelial cells.31 Conversely, Neumann et al. proposed that increased intracellular cAMP stabilizes the interaction between I-κBα and NF-κB, therefore reducing NF-κB activation.32 In contrast, Takahashi et al. showed no difference in I-κBα degradation in Jurkat T-lymphocytes in the presence and absence of forskolin, an alternative cAMP inducing compound.
T8392 21746-22290 Sentence denotes This group also demonstrated that forskolin did not inhibit the DNA-binding activity of NF-κB and that CREB was not involved in forskolin-induced decrease in p65 transcriptional activity.33 Similar results were reported by Ollivier et al. using THP-1 cells and endothelial cells treated with forskolin or dibutyryl cAMP, two agents known to function through cAMP elevation and subsequent PKA activation.9 The differences between our results and those mentioned above may be explained by the use of different cell types and exogenous stimulants.
T8393 22291-22547 Sentence denotes It is also possible that PTX exerts its anti-inflammatory effects on the NF-κB pathway independent of PKA, as suggested by the continued attenuation of LPS-induced TNF-α production by PTX in the presence or absence of PKA inhibition observed in this study.
T8394 22548-22615 Sentence denotes This hypothesis is currently under investigation in our laboratory.
T8395 22616-22775 Sentence denotes Furthermore, we utilized a broad PKA inhibitor in this study in addition to H89, but did not examine the effect of specific inhibition of various PKA isoforms.
T8396 22776-22883 Sentence denotes This information may yield more insight into the mechanism by which PTX exerts its down-regulatory effects.
T8397 22884-23056 Sentence denotes PDE inhibitors may also downregulate NF-κB transcriptional activity by altering the competitive binding of CREB and NF-κB to the promoter regions of pro-inflammatory genes.
T8398 23057-23177 Sentence denotes Herein, we showed that the activation and DNA binding activity of CREB is upregulated in a dose–dependent manner by PTX.
T8399 23178-23418 Sentence denotes Of note, the increase in CREB activity was slightly alleviated by the addition of LPS, suggesting competition between PTX-induced CREB-associated gene transcription and those factors, such as NF-κB, which may be upregulated by LPS exposure.
T8400 23419-23630 Sentence denotes This competition has been postulated to involve the recruitment of the co-activator CBP and its homologue p30034, suggesting an alternative mechanism that may be involved in the anti-inflammatory actions of PTX.
T8401 23631-23712 Sentence denotes Future studies are planned to delineate the validity of this potential mechanism.
T8402 23713-23835 Sentence denotes The effects of PTX on other transcription factors, such as AP-1, c-fos, and c-jun, cannot be ruled out by our experiments.
T8403 23836-23981 Sentence denotes Activation of AP-1 and its components (c-fos and c-jun) by agents that enhance mononuclear cell activity has been implicated in TNF-α expression.
T8404 23982-24090 Sentence denotes Moreover, AP-1 and CREB recognize a similar DNA binding sequence in the promoter region of the TNF-α gene35.
T8405 24091-24290 Sentence denotes Given these relationships, it is conceivable that PTX may exert some of its anti-inflammatory actions by affecting AP-1 activation and favoring CREB binding, thus inhibiting TNF-α gene transcription.
T8406 24291-24434 Sentence denotes Additional studies are necessary to elucidate the complex interactions between CREB, NF-κB, and AP-1 following LPS exposure and PDE inhibition.
T8407 24435-24571 Sentence denotes Furthermore, the effect of PTX on the cGMP pathway after LPS stimulation has not been explored, although we plan to do so in the future.
T8408 24572-24738 Sentence denotes In summary, we have demonstrated that PDE inhibition of human mononuclear cells downregulates TNF-α production, at least in part, through a PKA-independent mechanism.
T8409 24739-24916 Sentence denotes In addition, PTX attenuates the activity of NF-B while upregulating CREB activation after LPS stimulation, which may result in modulation of pro-inflammatory mediator synthesis.
T8410 24917-25060 Sentence denotes Therefore, PTX may serve as a potential adjunct therapeutic for the treatment of conditions in which TNF-α production plays a significant role.
T1 0-76 Sentence denotes Insights into the Regulation of TNF-α Production in Human Mononuclear Cells:
T2 77-133 Sentence denotes The Effects of Non-Specific Phosphodiesterase Inhibition
T3 136-144 Sentence denotes Abstract
T4 145-154 Sentence denotes OBJECTIVE
T5 155-404 Sentence denotes The objective of this study was to determine the effect of nonspecific phosphodiesterase inhibition on transcription factor activation and tumor necrosis factor-alpha (TNF-α) production in lipopolysaccharide (LPS)-stimulated human mononuclear cells.
T6 406-418 Sentence denotes INTRODUCTION
T7 419-530 Sentence denotes The production of TNF-α following LPS stimulation is one of the key steps in bacterial sepsis and inflammation.
T8 531-646 Sentence denotes The mechanism by which phosphodiesterase inhibition alters TNF-α production in the presence of LPS remains unclear.
T9 648-655 Sentence denotes METHODS
T10 656-818 Sentence denotes Human mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor.
T11 819-975 Sentence denotes Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed.
T12 976-1059 Sentence denotes DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay.
T13 1060-1232 Sentence denotes TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA).
T14 1234-1241 Sentence denotes RESULTS
T15 1242-1389 Sentence denotes PTX was demonstrated to significantly reduce cytoplasmic I-κBα phosphorylation, nuclear p65 phosphorylation, and the DNA binding activity of NF-κB.
T16 1390-1478 Sentence denotes In contrast, PTX markedly enhanced the phosphorylation and DNA binding activity of CREB.
T17 1479-1613 Sentence denotes Cells concomitantly treated with PTX and LPS secreted similar levels of TNF-α in the presence and absence Protein kinase A inhibition.
T18 1615-1625 Sentence denotes DISCUSSION
T19 1626-1899 Sentence denotes The increased level of cAMP that results from phosphodiesterase inhibition affects cytoplasmic and nuclear events, resulting in the attenuation of NF-κB and the activation of CREB transcriptional DNA binding through pathways that are partially Protein kinase A-independent.
T20 1901-1911 Sentence denotes CONCLUSION
T21 1912-2096 Sentence denotes PTX-mediated phosphodiesterase inhibition occurs partially through a Protein kinase A-independent pathway and may serve as a useful tool in the attenuation of LPS-induced inflammation.
T22 2099-2111 Sentence denotes INTRODUCTION
T23 2112-2299 Sentence denotes The bacterial membrane component lipopolysaccharide (LPS) is capable of initiating phosphorylation and activation of multiple host intracellular protein kinases and transcription factors.
T24 2300-2486 Sentence denotes Transcription factor activation, which results in the modulation of gene transcription and protein synthesis, is a critical element in the defense mechanism of the host immune system.1,2
T25 2487-2710 Sentence denotes LPS-induced transcription factor activation has been shown to be a key regulator of tumor necrosis factor-α (TNF-α) production.3 TNF-α is a potent pro-inflammatory cytokine involved in a wide spectrum of cellular responses.
T26 2711-3055 Sentence denotes Furthermore, TNF-α synthesis can be attenuated in immune cells exposed to phosphodiesterase (PDE) inhibition after challenge by a variety of pro-inflammatory stimulants.4 The signaling mechanisms affected by PDE inhibition, which ultimately lead to the downregulation of TNF-α production, have not been well characterized in inflammatory cells.
T27 3056-3683 Sentence denotes Classically, it has been demonstrated that PDE inhibition results in the intracellular accumulation of the second messenger cyclic adenosine-3,5-monophosphate (cAMP) and subsequent activation of Protein kinase A (PKA).5 PKA activation then leads to the phosphorylation of the transcription factor cAMP-response element binding protein (CREB), transmission of signals into the nucleus, and the subsequent modulation of gene transcription.6 This apparently simple linear cascade does not fully explain the mechanism by which an elevation in the intracellular cAMP level exerts wide-ranging effects on multiple cellular functions.
T28 3684-3812 Sentence denotes There is a growing body of evidence suggesting that cAMP may function through both PKA-dependent and -independent mechanisms.6–8
T29 3813-3942 Sentence denotes LPS-induced activation of the transcription factor nuclear factor-κB (NF-κB) has also been the focus of a great deal of research.
T30 3943-4119 Sentence denotes It has been clearly demonstrated that agents that increase intracellular cAMP also inhibit NF-κB-dependent pro-inflammatory gene transcription, particularly of the TNF-α gene.9
T31 4120-4228 Sentence denotes Controversy exists regarding the exact mechanism(s) by which PDE inhibition down-regulates TNF-α production.
T32 4229-4494 Sentence denotes Possibilities include, but are not limited to, inhibition of NF-κB DNA binding activity, downregulation of NF-κB transcriptional activity, increased CREB activation, and competition between NF-κB and CREB for common co-activators such as CREB binding protein (CBP).
T33 4495-4579 Sentence denotes It is also not known whether these processes rely solely upon the activation of PKA.
T34 4580-4812 Sentence denotes Therefore, the objective of the present study is to determine the effects of nonspecific PDE inhibition with 1-[5-oxohexyl]-3,7-dimethylxanthine (Pentoxifylline; PTX) on NF-κB and CREB activation in vitro in human mononuclear cells.
T35 4813-4919 Sentence denotes With the use of specific inhibitors, we also investigated the role of PKA in LPS-induced TNF-α production.
T36 4921-4942 Sentence denotes MATERIALS AND METHODS
T37 4943-5044 Sentence denotes This study was approved by the Human Research Protections Program and the Institutional Review Board.
T38 5045-5146 Sentence denotes Written consent to participate in the study was obtained from all volunteers prior to blood donation.
T39 5147-5223 Sentence denotes Heparinized Vacutainers were purchased from Becton Dickinson (San Jose, CA).
T40 5224-5312 Sentence denotes 1.5-mL Eppendorf centrifuge tubes were purchased from Fisher Scientific (Pittsburg, PA).
T41 5313-5417 Sentence denotes Hank’s balanced salt solution (HBSS) and RPMI 1640 were obtained from Irvine Scientific (Santa Ana, CA).
T42 5418-5511 Sentence denotes LPS from Escherichia coli serotype 0111:B4 and PTX were purchased from Sigma (St. Louis, MO).
T43 5512-5651 Sentence denotes The PKA inhibitor, N-[2-((p-bromocinnamyl)amino)ethyl]-5-isoguinolinesulfonamide-2HCl] (H89), was purchased from Calbiochem (La Jolla, CA).
T44 5652-5864 Sentence denotes Antibodies for phosphorylated CREB (serine 133), phosphorylated I-κBα (serines 32 and 36), phosphorylated NF-κB p65 subunit (serine 276), and secondary antibodies were purchased from Cell Signaling (Beverly, MA).
T45 5865-5961 Sentence denotes Dextran T500 and Percoll were received from GE Healthcare/Amersham Biosciences (Piscataway, NJ).
T46 5962-6077 Sentence denotes The enzyme-linked immunosorbent assay (ELISA) for TNF-α was obtained from Quantikine R&D Systems (Minneapolis, MN).
T47 6078-6221 Sentence denotes Tris-glycine and DNA retardation gels, nitrocellulose membranes, and western blot running buffers were obtained from Invitrogen (Carlsbad, CA).
T48 6222-6439 Sentence denotes NE-PER® nuclear and cytoplasmic extraction reagents, the BCA protein reagent kit, the Supersignal West Pico Chemiluminescent Kit, and the LightShift Chemiluminescent EMSA Kit were purchased from Pierce (Rockland, IL).
T49 6440-6523 Sentence denotes CREB and NF-κB DNA oligonucleotide probes were synthesized by IDT (Coralville, IA).
T50 6524-6602 Sentence denotes Nylon membranes were purchased from Roche Applied Sciences (Indianapolis, IN).
T51 6603-6722 Sentence denotes Western blot band quantification was performed with the UN-SCAN-IT Gel Digitizing software (Silk Scientific, Orem, UT).
T52 6724-6789 Sentence denotes Human Peripheral Blood Mononuclear Cell Isolation and Stimulation
T53 6790-6887 Sentence denotes Human mononuclear cells were isolated from the peripheral blood of four healthy human volunteers.
T54 6888-7093 Sentence denotes The sample size was chosen from our previously published work, which demonstrated reliable results.10 Cell isolation and all subsequent experiments were conducted under sterile and pyrogen-free conditions.
T55 7094-7228 Sentence denotes Blood collected in heparin tubes was incubated with Dextran T500 and the red cells were sedimented for 40 minutes at room temperature.
T56 7229-7369 Sentence denotes The resultant serum was then washed with HBSS and separated by Percoll gradient centrifugation according to the manufacturer’s instructions.
T57 7370-7460 Sentence denotes Cell viability was assessed by trypan blue dye exclusion, with purity of greater than 95%.
T58 7461-7585 Sentence denotes Isolated cells were resuspended in RPMI 1640 supplemented with 10% FBS and 5 mM HEPES at a concentration of 1 x 107cells/mL.
T59 7586-7688 Sentence denotes Each subsequent experiment listed below was conducted on samples of 5 x 106 cells per treatment group.
T60 7689-7876 Sentence denotes Isolated cells were stimulated with either HBSS as a negative control, LPS (1 μg/mL), PTX (20 mM), or concomitant LPS / PTX, at the above-mentioned concentrations, for 30 minutes at 37ºC.
T61 7877-8001 Sentence denotes Cells were placed on ice for 10 minutes to stop the reaction and the samples were then stored at −70°C for further analysis.
T62 8002-8242 Sentence denotes The stimulation times and concentrations of LPS and PTX utilized in this study were determined in previous pilot studies done in our laboratory, which examined the effects of increasing concentrations of PTX on LPS-induced TNF-α production.
T63 8243-8503 Sentence denotes LPS at a concentration of 1 μg/mL and PTX at a concentration of 20 mM were the minimum concentrations necessary to produce a reproducible and reliable up-regulation and down-regulation in TNF-α production, respectively, in quantitative assays (data not shown).
T64 8505-8571 Sentence denotes Effect of PTX on LPS-induced TNF-α production by mononuclear cells
T65 8572-8773 Sentence denotes The concentration of TNF-α was quantitatively determined by ELISA in the media of cells exposed to the treatments described above after stimulation for up to a maximum of 18 hours, at 3-hour intervals.
T66 8774-8809 Sentence denotes The results are expressed in pg/mL.
T67 8811-8867 Sentence denotes SDS-PAGE and Immunoblotting (I-κBα, NF-κB p65, and CREB)
T68 8868-8968 Sentence denotes Following stimulation, PBMCs were washed with ice-cold PBS and centrifuged to collect a cell pellet.
T69 8969-9066 Sentence denotes The pellet was resuspended in ice-cold SDS sample buffer supplemented with 100 mM dithiothreitol.
T70 9067-9256 Sentence denotes Cytoplasmic and nuclear extracts were isolated with NE-PER nuclear and cytoplasmic extraction reagents with 1x Halt Protease Inhibitor Cocktail according to the manufacturer’s instructions.
T71 9257-9391 Sentence denotes Protein concentrations were determined with the BCA protein reagent kit for each sample according to a standardized curve for albumin.
T72 9392-9479 Sentence denotes Cell lysates were collected by boiling the samples in a 100ºC water bath for 5 minutes.
T73 9480-9665 Sentence denotes Ten μg of protein per sample was separated by SDS-polyacrylamide gel electrophoresis through 8–16% tris-glycine polyacrylamide gradient gels and transferred to nitrocellulose membranes.
T74 9666-9787 Sentence denotes The membranes were blocked with 5% milk in Tris-buffered saline/Tween 20 (Fischer Scientific, Pittsburgh, PA) for 1 hour.
T75 9788-10041 Sentence denotes Cytoplasmic extracts were incubated with phosphorylated I-κBα antibody (1:200), while nuclear extracts were incubated with either phosphorylated NF-κB p65 antibody (1:500) or phosphorylated CREB antibody (1:500) overnight at 4°C in separate experiments.
T76 10042-10259 Sentence denotes The membranes were washed with Tris-buffered saline/Tween 20 and incubated for 1 hour at room temperature with the secondary antibody, horseradish peroxidase-linked anti-rabbit IgG diluted 1:2000 in blocking solution.
T77 10260-10418 Sentence denotes After repeated washing of the membrane, the Supersignal West Pico Chemiluminescent Kit was applied for antibody detection per the manufacturer’s instructions.
T78 10419-10485 Sentence denotes Western blot data is presented as a percentage of LPS stimulation.
T79 10486-10682 Sentence denotes The percentage of LPS stimulation was calculated by dividing the mean band pixel total for each treatment arm divided by the mean pixel total of samples stimulated with LPS and multiplying by 100.
T80 10683-10800 Sentence denotes Thus, LPS stimulation is reported as 100% and each of the treatment arms is reported as a percent of LPS stimulation.
T81 10802-10860 Sentence denotes NF-κB and CREB Electrophoretic Mobility Shift Assay (EMSA)
T82 10861-10962 Sentence denotes The non-radioactive LightShift Chemiluminescent EMSA Kit was used to detect DNA-protein interactions.
T83 10963-11202 Sentence denotes The NF-κB 3′ biotin end-labeled DNA oligonucleotide used as a probe for the EMSA was a 42-bp double stranded construct (5′-TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC – 3′) containing two tandemly repeated NF-κB binding sites (underlined).
T84 11203-11342 Sentence denotes Specificity was determined by a competition assay with the addition of 200 molar excess of unlabeled double stranded NF-κB oligonucleotide.
T85 11343-11550 Sentence denotes The CREB 3′ biotin end-labeled DNA oligonucleotide used as a probe for the EMSA was a 23-bp double stranded construct (5′-TTT TCG AGC TCTGACGTCAGA-GC – 3′) containing the CRE consensus sequence (underlined).
T86 11551-11689 Sentence denotes Specificity was determined by a competition assay with the addition of 200 molar excess of unlabeled double stranded CREB oligonucleotide.
T87 11690-11767 Sentence denotes Nuclear extracts (10 μg) were incubated with 5 nM NF-κB or CREB probe (NF-kB:
T88 11768-11874 Sentence denotes 1x binding buffer, 50 mM KCl, 1 mM EDTA, 1 mM DTT, 0.1% NP40, 10% glycerol, and 50 ng/μl poly dI-dC, CREB:
T89 11875-12096 Sentence denotes 1x binding buffer, 20 mM Tris, pH 7.5, 50 mM KCl, 1 mM EDTA, 1 mM DTT, 0.10% NP40, 6% glycerol, 0.1 mg/mL BSA and 50 ng/μl poly dI-dC) and were then electrophoresed through a 6% DNA retardation gel at 100V for 90 minutes.
T90 12097-12308 Sentence denotes The gels were electrophoretically transferred at 380mA for 1 hour on ice to a positively charged nylon membrane and immediately cross-linked for 15 minutes with a UV transilluminator equipped with a 312 nm bulb.
T91 12309-12448 Sentence denotes Streptavidin-horseradish peroxidase conjugate and the LightShift Chemiluminescent Substrate were used to detect the biotin end-labeled DNA.
T92 12449-12526 Sentence denotes The nylon membranes were exposed to x-ray film for 1–3 minutes for detection.
T93 12528-12588 Sentence denotes Role of PKA on LPS-induced mononuclear cell TNF-α production
T94 12589-12807 Sentence denotes To evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized.
T95 12808-12980 Sentence denotes Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC.
T96 12981-13299 Sentence denotes The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments.
T97 13300-13380 Sentence denotes The TNF-α concentration in the supernatant was measured quantitatively by ELISA.
T98 13381-13416 Sentence denotes The results are expressed in pg/mL.
T99 13418-13438 Sentence denotes Statistical Analysis
T100 13439-13561 Sentence denotes The experimental results obtained in this study were derived from four separate experiments with healthy volunteer donors.
T101 13562-13632 Sentence denotes Each assay was performed in duplicate or triplicate where appropriate.
T102 13633-13669 Sentence denotes Data is presented as the mean ± SEM.
T103 13670-13794 Sentence denotes Statistical differences between groups were determined by one-way analysis of variance (ANOVA) with a Bonferroni correction.
T104 13795-13844 Sentence denotes Statistical significance was defined as P < 0.05.
T105 13846-13853 Sentence denotes RESULTS
T106 13855-13905 Sentence denotes The effects of PTX on LPS-induced TNF-α production
T107 13906-14028 Sentence denotes To determine the duration of PDE inhibition on TNF-α production after LPS stimulation, time course studies were conducted.
T108 14029-14137 Sentence denotes In accordance with prior work, PTX had a rapid and sustained effect on TNF-α production in vitro (Figure 1).
T109 14139-14181 Sentence denotes PTX decreases LPS-induced NF-κB activation
T110 14182-14346 Sentence denotes Since phosphorylation leads to ubiquitination and degradation of I-κBα and subsequent nuclear translocation of NF-κB, we first examined the effects of PTX on I-κBα.
T111 14347-14487 Sentence denotes Cytoplasmic I-κBα phosphorylation was markedly increased following LPS stimulation when compared to control (100 ± 0 vs. 20 ± 18; P < 0.05).
T112 14488-14590 Sentence denotes The addition of PTX significantly downregulated LPS-induced I-κBα phosphorylation (P = 0.02; Figure 2)
T113 14591-14719 Sentence denotes In a similar fashion, nuclear NF-κB p65 phosphorylation was increased following LPS stimulation (100 ± 0 vs. 38 ± 20; P < 0.01).
T114 14720-14887 Sentence denotes PTX similarly decreased LPS-induced NF-κB p65 nuclear phosphorylation, a marker of NF-κB activation and nuclear translocation (100 ± 0 vs. 40 ± 6; P =0.03; Figure 3A).
T115 14888-15044 Sentence denotes EMSAs were then performed to verify the PTX-induced alterations in nuclear phosphorylation resulted in similar effects on the DNA binding activity of NF-κB.
T116 15045-15240 Sentence denotes The addition of PTX to LPS-stimulated mononuclear cells resulted in comparable downregulation of DNA binding activity, similar to the observed downregulation of NF-κB phosphorylation (Figure 3B).
T117 15242-15315 Sentence denotes PTX upregulates CREB phosphorylation and activation after LPS stimulation
T118 15316-15389 Sentence denotes Phosphorylation of nuclear CREB was used as a marker for CREB activation.
T119 15390-15496 Sentence denotes LPS stimulation caused a negligible increase in CREB phosphorylation when compared to control (Figure 4A).
T120 15497-15576 Sentence denotes PTX alone caused a marked increase in CREB phosphorylation (P < 0.01 vs. HBSS).
T121 15577-15739 Sentence denotes When LPS and PTX exposure occurred simultaneously, CREB phosphorylation was significantly higher than with LPS stimulation alone (543 ± 92 vs. 100 ± 0; P < 0.01).
T122 15740-15942 Sentence denotes In addition, the amount of CREB phosphorylation seen with concomitant LPS and PTX treatment was less than that seen with PTX alone, although this difference was not statistically significant (P = 0.08).
T123 15943-16063 Sentence denotes To determine if CREB-DNA binding was affected by PTX in a manner similar to CREB phosphorylation, an EMSA was performed.
T124 16064-16175 Sentence denotes Exposure of LPS-stimulated cells to PTX similarly increased CREB DNA-binding compared to LPS alone (Figure 4B).
T125 16177-16244 Sentence denotes Effect of PTX on TNF-α production in the presence of PKA inhibition
T126 16245-16447 Sentence denotes The role of PKA in PTX-induced downregulation of TNF-α production in LPS-stimulated mononuclear cells was assessed by treating mononuclear cells with H89, a PKA inhibitor, prior to LPS and PTX exposure.
T127 16448-16622 Sentence denotes The addition PTX to LPS-stimulated cells resulted in a reduction in TNF-α, similar to that observed in our previous experiments (529 pg/mL ± 112 vs. 37 pg/mL ± 10; P < 0.01).
T128 16623-16821 Sentence denotes Pre-incubation of cells with H89 prior to LPS and PTX exposure did not result in any significant modulation of TNF-α production when compared to that demonstrated with PTX alone (P = 0.2; Figure 5).
T129 16822-16965 Sentence denotes These results suggest that downregulation of TNF-α production by PTX in LPS-stimulated mononuclear cells is, at least in part, PKA-independent.
T130 16967-16977 Sentence denotes DISCUSSION
T131 16978-17564 Sentence denotes The exposure of inflammatory cells to a variety of extracellular stimuli results in the initiation of transcriptional and posttranscriptional events, which culminate in the production of pro-inflammatory mediators.11–14 TNF-α is released very early following shock and is considered an important mediator of the inflammatory cascade, since it regulates the synthesis of several other critical cytokines and chemokines.15 A clear relationship between increased TNF-α synthesis and the development of shock and multiple organ dysfunction has been observed in animal models of sepsis.15–17
T132 17565-17743 Sentence denotes Because of its importance in orchestrating multiple steps of the inflammatory response, strategies aimed at decreasing TNF-α levels or attenuating its production seem attractive.
T133 17744-17843 Sentence denotes PDE inhibitors have been shown to downregulate TNF-α production in distinct human cell populations.
T134 17844-18196 Sentence denotes We have previously shown that, in general, TNF-α levels continue to increase after LPS exposure and that concomitant infusion of phosphodiesterase inhibitors can markedly decrease TNF-α levels.18–19 In this in vitro study, we verified the sustained attenuation of LPS-induced TNF-α expression over time in an isolated human mononuclear cell population.
T135 18197-18364 Sentence denotes The mechanisms by which PTX, a non-specific phosphodiesterase inhibitor, significantly and consistently decreases TNF-α production have not been completely elucidated.
T136 18365-18500 Sentence denotes It has been postulated that drugs that increase intracellular cAMP exert their anti-inflammatory effects through the activation of PKA.
T137 18501-18908 Sentence denotes This classic pathway has been challenged as the sole mechanism involved in the modulation of inflammation by cAMP-enhancing drugs, and alternative mechanisms involving PKA-independent pathways have been proposed.6–8 In this series of experiments, we observed a significant attenuation of TNF-α production in LPS-stimulated human mononuclear cells following PTX administration, independent of PKA activation.
T138 18909-19332 Sentence denotes We used a nonspecific phosphodiesterase inhibitor, PTX, in the present study because it has been used clinically in the treatment of a variety of conditions in which inflammation is an important component of the pathophysiology of the disease process.19 Additionally, the use of PTX in sepsis as an adjunct to other treatments to maintain adequate organ function has been explored based upon its effects on TNF-α synthesis.
T139 19333-19834 Sentence denotes However, the reported beneficial effects of PTX are not only related to the downregulation of TNF-αbut also to PTX’s hemorrheologic properties, its ability to reduce neutrophil activation, and its beneficial effects on microcirculation, cardiac performance, and organ injury.20–25 Herein, we demonstrated that the downregulatory effects of PTX in human mononuclear cells involves modulation of pathways that involve NF-κB and CREB, two major transcription factors involved in the inflammatory cascade.
T140 19835-19998 Sentence denotes NF-κB/Rel constitutes a family of transcriptional factors involved in the regulation of numerous cytokine genes and immune responses in different cell populations.
T141 19999-20160 Sentence denotes The most abundant form of NF-κB is the p50–p65 heterodimer.26 The inactive form of NF-κB exists in the cytoplasm bound to an inhibitory complex containing I-κBα.
T142 20161-20386 Sentence denotes Following cellular stimulation with LPS and chemotactic factors, NF-κB inducible kinase phosphorylates and activates the I-κB kinase (IKK) complex consisting of IKK-1 and IKK-2, which in turn phosphorylates the I-κBα subunit.
T143 20387-20495 Sentence denotes Phosphorylation, ubiquitination, and subsequent degradation of I-κBα results in NF-κB nuclear translocation.
T144 20496-20849 Sentence denotes This allows the transcription factor to bind to promoter regions of specific pro-inflammatory genes and influence transcription.27,28 Because a number of potential κB sites are present in the nucleotide sequences of the TNF-α gene, NF-κB is considered to be a critical transcriptional factor involved in TNF-α gene expression and protein synthesis.29,30
T145 20850-21146 Sentence denotes In this set of experiments, we demonstrated that PTX downregulates cytoplasmic I-κBα phosphorylation, nuclear NF-κB p65 phosphorylation/translocation, as well as NF-κB DNA-binding after LPS stimulation, suggesting that PTX exerts its function proximal to or at the level of I-κBα phosphorylation.
T146 21147-21273 Sentence denotes Previous studies that have evaluated the effects of cAMP-elevating drugs on I-κBα and NF-κB have reported conflicting results.
T147 21274-21745 Sentence denotes Haddad et al. reported results similar to ours with respect to the effects of PTX on I-κBα and NF-κB in pulmonary epithelial cells.31 Conversely, Neumann et al. proposed that increased intracellular cAMP stabilizes the interaction between I-κBα and NF-κB, therefore reducing NF-κB activation.32 In contrast, Takahashi et al. showed no difference in I-κBα degradation in Jurkat T-lymphocytes in the presence and absence of forskolin, an alternative cAMP inducing compound.
T148 21746-22290 Sentence denotes This group also demonstrated that forskolin did not inhibit the DNA-binding activity of NF-κB and that CREB was not involved in forskolin-induced decrease in p65 transcriptional activity.33 Similar results were reported by Ollivier et al. using THP-1 cells and endothelial cells treated with forskolin or dibutyryl cAMP, two agents known to function through cAMP elevation and subsequent PKA activation.9 The differences between our results and those mentioned above may be explained by the use of different cell types and exogenous stimulants.
T149 22291-22547 Sentence denotes It is also possible that PTX exerts its anti-inflammatory effects on the NF-κB pathway independent of PKA, as suggested by the continued attenuation of LPS-induced TNF-α production by PTX in the presence or absence of PKA inhibition observed in this study.
T150 22548-22615 Sentence denotes This hypothesis is currently under investigation in our laboratory.
T151 22616-22775 Sentence denotes Furthermore, we utilized a broad PKA inhibitor in this study in addition to H89, but did not examine the effect of specific inhibition of various PKA isoforms.
T152 22776-22883 Sentence denotes This information may yield more insight into the mechanism by which PTX exerts its down-regulatory effects.
T153 22884-23056 Sentence denotes PDE inhibitors may also downregulate NF-κB transcriptional activity by altering the competitive binding of CREB and NF-κB to the promoter regions of pro-inflammatory genes.
T154 23057-23177 Sentence denotes Herein, we showed that the activation and DNA binding activity of CREB is upregulated in a dose–dependent manner by PTX.
T155 23178-23418 Sentence denotes Of note, the increase in CREB activity was slightly alleviated by the addition of LPS, suggesting competition between PTX-induced CREB-associated gene transcription and those factors, such as NF-κB, which may be upregulated by LPS exposure.
T156 23419-23630 Sentence denotes This competition has been postulated to involve the recruitment of the co-activator CBP and its homologue p30034, suggesting an alternative mechanism that may be involved in the anti-inflammatory actions of PTX.
T157 23631-23712 Sentence denotes Future studies are planned to delineate the validity of this potential mechanism.
T158 23713-23835 Sentence denotes The effects of PTX on other transcription factors, such as AP-1, c-fos, and c-jun, cannot be ruled out by our experiments.
T159 23836-23981 Sentence denotes Activation of AP-1 and its components (c-fos and c-jun) by agents that enhance mononuclear cell activity has been implicated in TNF-α expression.
T160 23982-24090 Sentence denotes Moreover, AP-1 and CREB recognize a similar DNA binding sequence in the promoter region of the TNF-α gene35.
T161 24091-24290 Sentence denotes Given these relationships, it is conceivable that PTX may exert some of its anti-inflammatory actions by affecting AP-1 activation and favoring CREB binding, thus inhibiting TNF-α gene transcription.
T162 24291-24434 Sentence denotes Additional studies are necessary to elucidate the complex interactions between CREB, NF-κB, and AP-1 following LPS exposure and PDE inhibition.
T163 24435-24571 Sentence denotes Furthermore, the effect of PTX on the cGMP pathway after LPS stimulation has not been explored, although we plan to do so in the future.
T164 24572-24738 Sentence denotes In summary, we have demonstrated that PDE inhibition of human mononuclear cells downregulates TNF-α production, at least in part, through a PKA-independent mechanism.
T165 24739-24916 Sentence denotes In addition, PTX attenuates the activity of NF-B while upregulating CREB activation after LPS stimulation, which may result in modulation of pro-inflammatory mediator synthesis.
T166 24917-25060 Sentence denotes Therefore, PTX may serve as a potential adjunct therapeutic for the treatment of conditions in which TNF-α production plays a significant role.

ICD10

Id Subject Object Predicate Lexical cue
T10026 17237-17242 http://purl.bioontology.org/ontology/ICD10/R57.9 denotes shock
T10027 17477-17482 http://purl.bioontology.org/ontology/ICD10/R57.9 denotes shock
T10028 19601-19607 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury

events-check-again

Id Subject Object Predicate Lexical cue Negation Speculation
T884 18-28 Regulation denotes Regulation
T885 32-37 Protein denotes TNF-α
T886 38-48 Gene_expression denotes Production
T887 204-210 Regulation denotes effect true
T888 294-321 Protein denotes tumor necrosis factor-alpha
T889 323-328 Protein denotes TNF-α
T890 330-340 Gene_expression denotes production
T891 423-433 Gene_expression denotes production
T892 437-442 Protein denotes TNF-α
T893 443-452 Positive_regulation denotes following
T894 583-589 Regulation denotes alters
T895 590-595 Protein denotes TNF-α
T896 596-606 Gene_expression denotes production
T897 607-625 Positive_regulation denotes in the presence of
T898 839-853 Phosphorylation denotes phosphorylated
T899 866-871 Protein denotes I-κBα
T900 891-894 Protein denotes p65
T901 1060-1065 Protein denotes TNF-α
T902 1280-1286 Negative_regulation denotes reduce
T903 1280-1286 Negative_regulation denotes reduce
T904 1299-1304 Protein denotes I-κBα
T905 1305-1320 Phosphorylation denotes phosphorylation
T906 1330-1333 Protein denotes p65
T907 1334-1349 Phosphorylation denotes phosphorylation
T908 1524-1532 Localization denotes secreted
T909 1551-1556 Protein denotes TNF-α
T910 1564-1572 Regulation denotes presence
T911 1577-1584 Regulation denotes absence true
T2369 2558-2567 Regulation denotes regulator
T2370 2571-2594 Protein denotes tumor necrosis factor-α
T2371 2596-2601 Protein denotes TNF-α
T2372 2603-2613 Gene_expression denotes production
T2373 2616-2621 Protein denotes TNF-α
T2374 2724-2729 Protein denotes TNF-α
T2375 2730-2739 Gene_expression denotes synthesis
T2376 2747-2757 Negative_regulation denotes attenuated
T2377 2952-2956 Positive_regulation denotes lead
T2378 2964-2978 Negative_regulation denotes downregulation
T2379 2982-2987 Protein denotes TNF-α
T2380 2988-2998 Gene_expression denotes production
T2381 4026-4033 Negative_regulation denotes inhibit
T2382 4040-4049 Regulation denotes dependent
T2383 4072-4085 Transcription denotes transcription
T2384 4107-4112 Protein denotes TNF-α
T2385 4196-4210 Negative_regulation denotes down-regulates
T2386 4211-4216 Protein denotes TNF-α
T2387 4217-4227 Gene_expression denotes production
T2388 4875-4879 Regulation denotes role true
T2389 4894-4901 Positive_regulation denotes induced
T2390 4902-4907 Protein denotes TNF-α
T2391 4908-4918 Gene_expression denotes production
T3205 5667-5681 Phosphorylation denotes phosphorylated
T3206 5682-5686 Protein denotes CREB
T3207 5688-5698 Entity denotes serine 133
T3208 5701-5715 Phosphorylation denotes phosphorylated
T3209 5701-5715 Phosphorylation denotes phosphorylated
T3210 5716-5721 Protein denotes I-κBα
T3211 5723-5733 Entity denotes serines 32
T3212 5738-5740 Entity denotes 36
T3213 5743-5757 Phosphorylation denotes phosphorylated
T3214 5764-5767 Protein denotes p65
T3215 5777-5787 Entity denotes serine 276
T3216 6012-6017 Protein denotes TNF-α
T3941 8217-8224 Positive_regulation denotes induced
T3942 8225-8230 Protein denotes TNF-α
T3943 8231-8241 Gene_expression denotes production
T3944 8394-8407 Positive_regulation denotes up-regulation
T3945 8412-8427 Negative_regulation denotes down-regulation
T3946 8431-8436 Protein denotes TNF-α
T3947 8437-8447 Gene_expression denotes production
T4106 8526-8533 Positive_regulation denotes induced
T4107 8534-8539 Protein denotes TNF-α
T4108 8540-8550 Gene_expression denotes production
T4109 8593-8598 Protein denotes TNF-α
T4881 8840-8845 Protein denotes I-κBα
T4882 8853-8856 Protein denotes p65
T4883 8862-8866 Protein denotes CREB
T4884 9829-9843 Phosphorylation denotes phosphorylated
T4885 9844-9849 Protein denotes I-κBα
T4886 9918-9932 Phosphorylation denotes phosphorylated
T4887 9939-9942 Protein denotes p65
T6040 12528-12532 Regulation denotes Role
T6041 12547-12554 Positive_regulation denotes induced
T6042 12572-12577 Protein denotes TNF-α
T6043 12578-12588 Gene_expression denotes production
T6044 12605-12609 Regulation denotes role
T6045 12620-12625 Protein denotes TNF-α
T6046 12626-12636 Gene_expression denotes production
T6047 12666-12677 Regulation denotes involvement
T6048 12685-12696 Negative_regulation denotes attenuation
T6049 12704-12711 Positive_regulation denotes induced
T6050 12712-12717 Protein denotes TNF-α
T6051 12718-12728 Gene_expression denotes production
T6052 13304-13309 Protein denotes TNF-α
T6411 13859-13866 Regulation denotes effects true
T6412 13881-13888 Positive_regulation denotes induced
T6413 13889-13894 Protein denotes TNF-α
T6414 13895-13905 Gene_expression denotes production
T6415 13953-13958 Protein denotes TNF-α
T6416 13959-13969 Gene_expression denotes production
T6417 13980-13991 Positive_regulation denotes stimulation
T6418 14090-14096 Regulation denotes effect
T6419 14100-14105 Protein denotes TNF-α
T6420 14106-14116 Gene_expression denotes production
T6929 14204-14209 Positive_regulation denotes leads
T6930 14232-14243 Protein_catabolism denotes degradation
T6931 14247-14252 Protein denotes I-κBα
T6932 14322-14329 Regulation denotes effects true
T6933 14340-14345 Protein denotes I-κBα
T6934 14359-14364 Protein denotes I-κBα
T6935 14365-14380 Phosphorylation denotes phosphorylation
T6936 14394-14403 Positive_regulation denotes increased
T6937 14404-14413 Positive_regulation denotes following
T6938 14522-14535 Negative_regulation denotes downregulated
T6939 14540-14547 Positive_regulation denotes induced
T6940 14548-14553 Protein denotes I-κBα
T6941 14554-14569 Phosphorylation denotes phosphorylation
T6942 14627-14630 Protein denotes p65
T6943 14631-14646 Phosphorylation denotes phosphorylation
T6944 14651-14660 Positive_regulation denotes increased
T6945 14661-14670 Positive_regulation denotes following
T6946 14734-14743 Negative_regulation denotes decreased
T6947 14748-14755 Positive_regulation denotes induced
T6948 14762-14765 Protein denotes p65
T6949 14774-14789 Phosphorylation denotes phosphorylation
T7544 16114-16123 Positive_regulation denotes increased
T7545 16124-16128 Protein denotes CREB
T7546 16133-16140 Binding denotes binding
T7957 16177-16183 Regulation denotes Effect true
T7958 16194-16199 Protein denotes TNF-α
T7959 16200-16210 Gene_expression denotes production
T7960 16211-16229 Regulation denotes in the presence of
T7961 16249-16253 Regulation denotes role
T7962 16268-16275 Positive_regulation denotes induced
T7963 16276-16290 Negative_regulation denotes downregulation
T7964 16294-16299 Protein denotes TNF-α
T7965 16300-16310 Gene_expression denotes production
T7966 16489-16497 Positive_regulation denotes resulted
T7967 16503-16512 Negative_regulation denotes reduction
T7968 16516-16521 Protein denotes TNF-α
T7969 16694-16700 Regulation denotes result true
T7970 16720-16730 Regulation denotes modulation
T7971 16734-16739 Protein denotes TNF-α
T7972 16740-16750 Gene_expression denotes production
T7973 16849-16863 Negative_regulation denotes downregulation
T7974 16867-16872 Protein denotes TNF-α
T7975 16873-16883 Gene_expression denotes production
T7976 16953-16964 Regulation denotes independent true
T10083 17198-17203 Protein denotes TNF-α
T10084 17207-17215 Localization denotes released
T10085 17227-17236 Positive_regulation denotes following
T10086 17428-17437 Positive_regulation denotes increased
T10087 17438-17443 Protein denotes TNF-α
T10088 17444-17453 Gene_expression denotes synthesis
T10089 17673-17683 Negative_regulation denotes decreasing
T10090 17684-17689 Protein denotes TNF-α
T10091 17700-17711 Negative_regulation denotes attenuating
T10092 17716-17726 Gene_expression denotes production
T10093 17778-17790 Negative_regulation denotes downregulate
T10094 17791-17796 Protein denotes TNF-α
T10095 17797-17807 Gene_expression denotes production
T10096 17887-17892 Protein denotes TNF-α
T10097 17912-17920 Positive_regulation denotes increase
T10098 18015-18023 Negative_regulation denotes decrease
T10099 18024-18029 Protein denotes TNF-α
T10100 18093-18104 Negative_regulation denotes attenuation
T10101 18112-18119 Positive_regulation denotes induced
T10102 18120-18125 Protein denotes TNF-α
T10103 18126-18136 Gene_expression denotes expression
T10104 18301-18310 Negative_regulation denotes decreases true
T10105 18311-18316 Protein denotes TNF-α
T10106 18317-18327 Gene_expression denotes production
T10107 18774-18785 Negative_regulation denotes attenuation
T10108 18789-18794 Protein denotes TNF-α
T10585 18795-18805 Gene_expression denotes production
T10586 18848-18857 Positive_regulation denotes following
T10587 18878-18889 Regulation denotes independent true
T10588 19305-19312 Regulation denotes effects
T10589 19316-19321 Protein denotes TNF-α
T10590 19322-19331 Gene_expression denotes synthesis
T10591 19409-19423 Negative_regulation denotes downregulation
T10592 19427-19432 Protein denotes TNF-α
T10593 20038-20041 Protein denotes p50
T10594 20042-20045 Protein denotes p65
T10595 20154-20159 Protein denotes I-κBα
T10596 20322-20327 Protein denotes IKK-1
T10597 20332-20337 Protein denotes IKK-2
T10598 20353-20367 Phosphorylation denotes phosphorylates
T10599 20372-20377 Protein denotes I-κBα
T10600 20387-20402 Phosphorylation denotes Phosphorylation
T10601 20404-20418 Ubiquitination denotes ubiquitination
T10602 20435-20446 Protein_catabolism denotes degradation
T10603 20450-20455 Protein denotes I-κBα
T10604 20716-20721 Protein denotes TNF-α
T10605 20788-20796 Regulation denotes involved true
T10606 20800-20805 Protein denotes TNF-α
T10607 20806-20821 Gene_expression denotes gene expression
T10608 20903-20916 Negative_regulation denotes downregulates
T10609 20903-20916 Negative_regulation denotes downregulates
T10610 20903-20916 Negative_regulation denotes downregulates
T10611 20929-20934 Protein denotes I-κBα
T10612 20935-20950 Phosphorylation denotes phosphorylation
T10613 20966-20969 Protein denotes p65
T10614 20970-20985 Phosphorylation denotes phosphorylation
T10615 20986-20999 Localization denotes translocation
T10616 21124-21129 Protein denotes I-κBα
T10617 21130-21145 Phosphorylation denotes phosphorylation
T10618 21188-21195 Regulation denotes effects
T10619 21223-21228 Protein denotes I-κBα
T10620 21341-21348 Regulation denotes effects
T10621 21359-21364 Protein denotes I-κBα
T10622 21478-21488 Regulation denotes stabilizes
T10623 21493-21504 Binding denotes interaction
T10624 21513-21518 Protein denotes I-κBα
T10625 21623-21628 Protein denotes I-κBα
T10626 21629-21640 Protein_catabolism denotes degradation
T10627 21672-21680 Regulation denotes presence
T10628 21685-21692 Regulation denotes absence true
T10629 21904-21907 Protein denotes p65
T10630 22428-22439 Negative_regulation denotes attenuation
T10631 22447-22454 Positive_regulation denotes induced
T10632 22455-22460 Protein denotes TNF-α
T10633 22461-22471 Gene_expression denotes production
T10634 22486-22505 Regulation denotes presence or absence true
T10635 23503-23506 Protein denotes CBP
T10636 23525-23529 Protein denotes p300
T10637 23778-23783 Protein denotes c-fos
T10638 23789-23794 Protein denotes c-jun
T10639 23836-23846 Positive_regulation denotes Activation
T10640 23836-23846 Positive_regulation denotes Activation
T10641 23875-23880 Protein denotes c-fos
T10642 23885-23890 Protein denotes c-jun
T10643 23950-23960 Regulation denotes implicated
T10644 23964-23969 Protein denotes TNF-α
T10645 23970-23980 Gene_expression denotes expression
T10646 24006-24015 Binding denotes recognize
T10647 24026-24046 Entity denotes DNA binding sequence
T10648 24077-24082 Protein denotes TNF-α
T10649 24254-24264 Negative_regulation denotes inhibiting
T10650 24265-24270 Protein denotes TNF-α
T10651 24276-24289 Transcription denotes transcription
T10652 24652-24665 Negative_regulation denotes downregulates
T10653 24666-24671 Protein denotes TNF-α
T10654 24672-24682 Gene_expression denotes production
T10655 24716-24727 Regulation denotes independent true
T10656 25018-25023 Protein denotes TNF-α
T10657 25024-25034 Gene_expression denotes production
R648 T885 T886 themeOf TNF-α,Production
R649 T886 T884 themeOf Production,Regulation
R650 T888 T890 themeOf tumor necrosis factor-alpha,production
R651 T890 T887 themeOf production,effect
R652 T891 T893 themeOf production,following
R653 T892 T891 themeOf TNF-α,production
R654 T894 T897 themeOf alters,in the presence of
R655 T895 T896 themeOf TNF-α,production
R656 T896 T894 themeOf production,alters
R657 T899 T898 themeOf I-κBα,phosphorylated
R658 T904 T905 themeOf I-κBα,phosphorylation
R659 T905 T903 themeOf phosphorylation,reduce
R660 T906 T907 themeOf p65,phosphorylation
R661 T907 T902 themeOf phosphorylation,reduce
R662 T908 T910 themeOf secreted,presence
R663 T908 T911 themeOf secreted,absence
R664 T909 T908 themeOf TNF-α,secreted
R1831 T2370 T2372 themeOf tumor necrosis factor-α,production
R1832 T2371 T2370 equivalentTo TNF-α,tumor necrosis factor-α
R1833 T2372 T2369 themeOf production,regulator
R1834 T2374 T2375 themeOf TNF-α,synthesis
R1836 T2375 T2376 themeOf synthesis,attenuated
R1838 T2378 T2377 themeOf downregulation,lead
R1839 T2379 T2380 themeOf TNF-α,production
R1840 T2380 T2378 themeOf production,downregulation
R1842 T2382 T2381 themeOf dependent,inhibit
R1843 T2383 T2382 themeOf transcription,dependent
R1845 T2384 T2383 themeOf TNF-α,transcription
R1846 T2386 T2387 themeOf TNF-α,production
R1848 T2387 T2385 themeOf production,down-regulates
R1849 T2389 T2388 themeOf induced,role
R1850 T2390 T2391 themeOf TNF-α,production
R1853 T2391 T2389 themeOf production,induced
R2569 T3207 T3205 themeOf serine 133,phosphorylated
R2570 T3207 T3206 partOf serine 133,CREB
R2571 T3211 T3209 themeOf serines 32,phosphorylated
R2572 T3211 T3210 partOf serines 32,I-κBα
R2573 T3212 T3208 themeOf 36,phosphorylated
R2574 T3212 T3210 partOf 36,I-κBα
R2575 T3215 T3213 themeOf serine 276,phosphorylated
R2576 T3215 T3214 partOf serine 276,p65
R3234 T3942 T3943 themeOf TNF-α,production
R3235 T3943 T3941 themeOf production,induced
R3236 T3946 T3947 themeOf TNF-α,production
R3237 T3947 T3944 themeOf production,up-regulation
R3238 T3947 T3945 themeOf production,down-regulation
R3363 T4107 T4108 themeOf TNF-α,production
R3364 T4108 T4106 themeOf production,induced
R4030 T4885 T4884 themeOf I-κBα,phosphorylated
R4031 T4887 T4886 themeOf p65,phosphorylated
R5017 T6041 T6040 themeOf induced,Role
R5018 T6042 T6043 themeOf TNF-α,production
R5019 T6043 T6041 themeOf production,induced
R5020 T6045 T6046 themeOf TNF-α,production
R5021 T6046 T6044 themeOf production,role
R5022 T6048 T6047 themeOf attenuation,involvement
R5023 T6049 T6048 themeOf induced,attenuation
R5024 T6050 T6051 themeOf TNF-α,production
R5025 T6051 T6049 themeOf production,induced
R5320 T6412 T6411 themeOf induced,effects
R5321 T6413 T6414 themeOf TNF-α,production
R5322 T6414 T6412 themeOf production,induced
R5323 T6415 T6416 themeOf TNF-α,production
R5324 T6416 T6417 themeOf production,stimulation
R5325 T6419 T6420 themeOf TNF-α,production
R5326 T6420 T6418 themeOf production,effect
R5767 T6930 T6929 themeOf degradation,leads
R5768 T6931 T6930 themeOf I-κBα,degradation
R5769 T6933 T6932 themeOf I-κBα,effects
R5770 T6934 T6935 themeOf I-κBα,phosphorylation
R5771 T6935 T6936 themeOf phosphorylation,increased
R5772 T6936 T6937 themeOf increased,following
R5773 T6939 T6938 themeOf induced,downregulated
R5774 T6940 T6941 themeOf I-κBα,phosphorylation
R5775 T6941 T6939 themeOf phosphorylation,induced
R5776 T6942 T6943 themeOf p65,phosphorylation
R5777 T6943 T6944 themeOf phosphorylation,increased
R5778 T6944 T6945 themeOf increased,following
R5779 T6947 T6946 themeOf induced,decreased
R5780 T6948 T6949 themeOf p65,phosphorylation
R5781 T6949 T6947 themeOf phosphorylation,induced
R6228 T7545 T7546 themeOf CREB,binding
R6229 T7546 T7544 themeOf binding,increased
R6571 T7957 T7960 themeOf Effect,in the presence of
R6572 T7958 T7959 themeOf TNF-α,production
R6573 T7959 T7957 themeOf production,Effect
R6574 T7962 T7961 themeOf induced,role
R6575 T7963 T7962 themeOf downregulation,induced
R6576 T7964 T7965 themeOf TNF-α,production
R6577 T7965 T7963 themeOf production,downregulation
R6578 T7967 T7966 themeOf reduction,resulted
R6579 T7968 T7967 themeOf TNF-α,reduction
R6580 T7970 T7969 themeOf modulation,result
R6581 T7971 T7972 themeOf TNF-α,production
R6582 T7972 T7970 themeOf production,modulation
R6583 T7973 T7976 themeOf downregulation,independent
R6584 T7974 T7975 themeOf TNF-α,production
R6585 T7975 T7973 themeOf production,downregulation
R8182 T10083 T10084 themeOf TNF-α,released
R8183 T10084 T10085 themeOf released,following
R8184 T10087 T10088 themeOf TNF-α,synthesis
R8185 T10088 T10086 themeOf synthesis,increased
R8186 T10090 T10092 themeOf TNF-α,production
R8187 T10090 T10089 themeOf TNF-α,decreasing
R8188 T10092 T10091 themeOf production,attenuating
R8189 T10094 T10095 themeOf TNF-α,production
R8190 T10095 T10093 themeOf production,downregulate
R8191 T10096 T10097 themeOf TNF-α,increase
R8192 T10099 T10098 themeOf TNF-α,decrease
R8193 T10101 T10100 themeOf induced,attenuation
R8194 T10102 T10103 themeOf TNF-α,expression
R8195 T10103 T10101 themeOf expression,induced
R8196 T10105 T10106 themeOf TNF-α,production
R8197 T10106 T10104 themeOf production,decreases
R8198 T10107 T10586 themeOf attenuation,following
R8199 T10107 T10587 themeOf attenuation,independent
R8200 T10108 T10585 themeOf TNF-α,production
R8432 T10585 T10107 themeOf production,attenuation
R8437 T10589 T10590 themeOf TNF-α,synthesis
R8442 T10590 T10588 themeOf synthesis,effects
R8448 T10592 T10591 themeOf TNF-α,downregulation
R8450 T10599 T10598 themeOf I-κBα,phosphorylates
R8457 T10603 T10600 themeOf I-κBα,Phosphorylation
R8462 T10603 T10601 themeOf I-κBα,ubiquitination
R8463 T10603 T10602 themeOf I-κBα,degradation
R8464 T10606 T10607 themeOf TNF-α,gene expression
R8467 T10607 T10605 themeOf gene expression,involved
R8470 T10611 T10612 themeOf I-κBα,phosphorylation
R8477 T10612 T10609 themeOf phosphorylation,downregulates
R8479 T10613 T10614 themeOf p65,phosphorylation
R8480 T10613 T10615 themeOf p65,translocation
R8481 T10614 T10608 themeOf phosphorylation,downregulates
R8482 T10615 T10610 themeOf translocation,downregulates
R8483 T10616 T10617 themeOf I-κBα,phosphorylation
R8484 T10619 T10618 themeOf I-κBα,effects
R8485 T10621 T10620 themeOf I-κBα,effects
R8487 T10623 T10622 themeOf interaction,stabilizes
R8488 T10624 T10623 themeOf I-κBα,interaction
R8489 T10625 T10626 themeOf I-κBα,degradation
R8490 T10626 T10627 themeOf degradation,presence
R8491 T10626 T10628 themeOf degradation,absence
R8493 T10630 T10634 themeOf attenuation,presence or absence
R8494 T10631 T10630 themeOf induced,attenuation
R8495 T10632 T10633 themeOf TNF-α,production
R8496 T10633 T10631 themeOf production,induced
R8499 T10641 T10639 themeOf c-fos,Activation
R8501 T10642 T10640 themeOf c-jun,Activation
R8502 T10644 T10645 themeOf TNF-α,expression
R8504 T10645 T10643 themeOf expression,implicated
R8506 T10647 T10646 themeOf DNA binding sequence,recognize
R8508 T10647 T10648 partOf DNA binding sequence,TNF-α
R8512 T10650 T10651 themeOf TNF-α,transcription
R8513 T10651 T10649 themeOf transcription,inhibiting
R8515 T10652 T10655 themeOf downregulates,independent
R8516 T10653 T10654 themeOf TNF-α,production
R8517 T10654 T10652 themeOf production,downregulates
R8519 T10656 T10657 themeOf TNF-α,production

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T912 32-37 Protein denotes TNF-α
T913 92-95 Protein denotes Non
T914 96-122 Protein denotes Specific Phosphodiesterase
T915 38-48 Gene_expression denotes Production
T916 123-133 Negative_regulation denotes Inhibition
T917 18-28 Regulation denotes Regulation
T918 81-88 Regulation denotes Effects
T919 294-321 Protein denotes tumor necrosis factor-alpha
T920 323-328 Protein denotes TNF-α
T921 330-340 Gene_expression denotes production
T922 330-340 Gene_expression denotes production
T923 279-289 Positive_regulation denotes activation
T924 279-289 Positive_regulation denotes activation
T925 204-210 Regulation denotes effect
T926 204-210 Regulation denotes effect
T927 437-442 Protein denotes TNF-α
T928 423-433 Gene_expression denotes production
T929 457-468 Positive_regulation denotes stimulation
T930 590-595 Protein denotes TNF-α
T931 596-606 Gene_expression denotes production
T932 583-589 Regulation denotes alters
T933 839-871 Protein denotes phosphorylated cytoplasmic I-κBα
T934 873-890 Protein denotes nuclear factor-κB
T935 891-894 Protein denotes p65
T936 896-901 Protein denotes NF-κB
T937 908-953 Protein denotes nuclear cAMP-response element binding protein
T938 955-959 Protein denotes CREB
T939 991-996 Protein denotes NF-κB
T940 1001-1005 Protein denotes CREB
T941 980-987 Binding denotes binding
T942 980-987 Binding denotes binding
T943 1060-1065 Protein denotes TNF-α
T944 1156-1172 Protein denotes Protein kinase A
T945 1173-1183 Negative_regulation denotes inhibition
T946 1242-1245 Protein denotes PTX
T947 1287-1300 Protein denotes cytoplasmic I
T948 1301-1304 Protein denotes κBα
T949 1330-1333 Protein denotes p65
T950 1383-1388 Protein denotes NF-κB
T951 1305-1320 Phosphorylation denotes phosphorylation
T952 1305-1320 Phosphorylation denotes phosphorylation
T953 1322-1329 Entity denotes nuclear
T954 1334-1349 Phosphorylation denotes phosphorylation
T955 1363-1370 Binding denotes binding
T956 1280-1286 Negative_regulation denotes reduce
T957 1403-1406 Protein denotes PTX
T958 1473-1477 Protein denotes CREB
T959 1429-1444 Phosphorylation denotes phosphorylation
T960 1416-1424 Positive_regulation denotes enhanced
T961 1512-1515 Protein denotes PTX
T962 1551-1556 Protein denotes TNF-α
T963 1585-1601 Protein denotes Protein kinase A
T964 1524-1532 Localization denotes secreted
T965 1524-1532 Localization denotes secreted
T966 1602-1612 Negative_regulation denotes inhibition
T967 1773-1778 Protein denotes NF-κB
T968 1801-1805 Protein denotes CREB
T969 1826-1833 Binding denotes binding
T970 1787-1797 Positive_regulation denotes activation
T971 1912-1915 Protein denotes PTX
T972 1981-1997 Protein denotes Protein kinase A
T2232 2229-2298 Protein denotes multiple host intracellular protein kinases and transcription factors
T2233 2195-2210 Phosphorylation denotes phosphorylation
T2234 2215-2225 Positive_regulation denotes activation
T2235 2184-2194 Positive_regulation denotes initiating
T2236 2184-2194 Positive_regulation denotes initiating
T2237 2571-2594 Protein denotes tumor necrosis factor-α
T2238 2596-2601 Protein denotes TNF-α
T2239 2616-2621 Protein denotes TNF-α
T2240 2558-2567 Regulation denotes regulator
T2241 2558-2567 Regulation denotes regulator
T2242 2724-2729 Protein denotes TNF-α
T2243 2804-2807 Protein denotes PDE
T2244 2982-2987 Protein denotes TNF-α
T2245 2785-2802 Protein denotes phosphodiesterase
T2246 2730-2739 Gene_expression denotes synthesis
T2247 2747-2757 Negative_regulation denotes attenuated
T2248 2809-2819 Negative_regulation denotes inhibition
T2249 2809-2819 Negative_regulation denotes inhibition
T2250 2988-2998 Gene_expression denotes production
T2251 2964-2978 Negative_regulation denotes downregulation
T2252 3251-3267 Protein denotes Protein kinase A
T2253 3269-3272 Protein denotes PKA
T2254 3276-3279 Protein denotes PKA
T2255 3353-3390 Protein denotes cAMP-response element binding protein
T2256 3392-3396 Protein denotes CREB
T2257 3143-3155 Positive_regulation denotes accumulation
T2258 3237-3247 Positive_regulation denotes activation
T2259 3280-3290 Positive_regulation denotes activation
T2260 3280-3290 Positive_regulation denotes activation
T2261 3296-3301 Positive_regulation denotes leads
T2262 3296-3301 Positive_regulation denotes leads
T2263 3309-3324 Phosphorylation denotes phosphorylation
T2264 3309-3324 Phosphorylation denotes phosphorylation
T2265 3114-3121 Positive_regulation denotes results
T2266 3767-3770 Protein denotes PKA
T2267 3843-3881 Protein denotes transcription factor nuclear factor-κB
T2268 3883-3888 Protein denotes NF-κB
T2269 3825-3835 Positive_regulation denotes activation
T2270 3825-3835 Positive_regulation denotes activation
T2271 4034-4039 Protein denotes NF-κB
T2272 4107-4112 Protein denotes TNF-α
T2273 4072-4085 Transcription denotes transcription
T2274 4072-4085 Transcription denotes transcription
T2275 4026-4033 Negative_regulation denotes inhibit
T2276 4026-4033 Negative_regulation denotes inhibit
T2277 4181-4184 Protein denotes PDE
T2278 4211-4216 Protein denotes TNF-α
T2279 4185-4195 Negative_regulation denotes inhibition
T2280 4217-4227 Gene_expression denotes production
T2281 4196-4210 Negative_regulation denotes down-regulates
T2282 4290-4295 Protein denotes NF-κB
T2283 4336-4341 Protein denotes NF-κB
T2284 4378-4382 Protein denotes CREB
T2285 4419-4424 Protein denotes NF-κB
T2286 4429-4433 Protein denotes CREB
T2287 4467-4487 Protein denotes CREB binding protein
T2288 4489-4492 Protein denotes CBP
T2289 4300-4307 Binding denotes binding
T2290 4383-4393 Positive_regulation denotes activation
T2291 4399-4410 Binding denotes competition
T2292 4276-4286 Negative_regulation denotes inhibition
T2293 4575-4578 Protein denotes PKA
T2294 4561-4571 Positive_regulation denotes activation
T2295 4750-4755 Protein denotes NF-κB
T2296 4760-4764 Protein denotes CREB
T2297 4765-4775 Positive_regulation denotes activation
T2298 4765-4775 Positive_regulation denotes activation
T2299 4883-4886 Protein denotes PKA
T2300 4902-4907 Protein denotes TNF-α
T2301 4908-4918 Gene_expression denotes production
T2302 4875-4879 Positive_regulation denotes role
T2303 4890-4901 Positive_regulation denotes LPS-induced
T3156 5465-5468 Protein denotes PTX
T3157 5516-5519 Protein denotes PKA
T3158 5682-5686 Protein denotes CREB
T3159 5701-5721 Protein denotes phosphorylated I-κBα
T3160 5758-5763 Protein denotes NF-κB
T3161 5764-5775 Protein denotes p65 subunit
T3162 5667-5681 Phosphorylation denotes phosphorylated
T3163 5743-5757 Phosphorylation denotes phosphorylated
T3164 5743-5757 Phosphorylation denotes phosphorylated
T3165 6012-6017 Protein denotes TNF-α
T3166 6279-6290 Protein denotes BCA protein
T3167 6440-6444 Protein denotes CREB
T3168 6449-6474 Protein denotes NF-κB DNA oligonucleotide
T3169 6487-6498 Gene_expression denotes synthesized
T3170 6487-6498 Gene_expression denotes synthesized
T3907 7496-7500 Protein denotes RPMI
T3908 7809-7812 Protein denotes PTX
T3909 8206-8209 Protein denotes PTX
T3910 8225-8230 Protein denotes TNF-α
T3911 8231-8241 Gene_expression denotes production
T3912 8166-8173 Regulation denotes effects
T3913 8281-8284 Protein denotes PTX
T3914 8431-8436 Protein denotes TNF-α
T3915 8437-8447 Gene_expression denotes production
T3916 8394-8407 Positive_regulation denotes up-regulation
T3917 8412-8427 Negative_regulation denotes down-regulation
T3918 8412-8427 Negative_regulation denotes down-regulation
T3919 8394-8407 Positive_regulation denotes up-regulation
T3920 8394-8407 Positive_regulation denotes up-regulation
T4088 8515-8518 Protein denotes PTX
T4089 8534-8539 Protein denotes TNF-α
T4090 8540-8550 Gene_expression denotes production
T4091 8505-8511 Regulation denotes Effect
T4092 8522-8533 Positive_regulation denotes LPS-induced
T4093 8593-8598 Protein denotes TNF-α
T4848 8847-8856 Protein denotes NF-κB p65
T4849 8862-8866 Protein denotes CREB
T4850 9175-9201 Protein denotes 1x Halt Protease Inhibitor
T4851 9305-9316 Protein denotes BCA protein
T4852 9383-9390 Protein denotes albumin
T4853 9844-9858 Protein denotes I-κBα antibody
T4854 9933-9951 Protein denotes NF-κB p65 antibody
T4855 9978-9982 Protein denotes CREB
T4856 9829-9843 Phosphorylation denotes phosphorylated
T4857 9963-9977 Phosphorylation denotes phosphorylated
T4858 9963-9977 Phosphorylation denotes phosphorylated
T4859 10177-10222 Protein denotes horseradish peroxidase-linked anti-rabbit IgG
T5584 10802-10807 Protein denotes NF-κB
T5585 10812-10816 Protein denotes CREB
T5586 10967-10974 Protein denotes NF-κB 3
T5587 10976-10982 Protein denotes biotin
T5588 11169-11174 Protein denotes NF-κB
T5589 11320-11341 Protein denotes NF-κB oligonucleotide
T5590 11347-11361 Protein denotes CREB 3′ biotin
T5591 11668-11672 Protein denotes CREB
T5592 11740-11745 Protein denotes NF-κB
T5593 11749-11753 Protein denotes CREB
T5594 11862-11867 Protein denotes dI-dC
T5595 11869-11877 Protein denotes CREB: 1x
T5596 12309-12344 Protein denotes Streptavidin-horseradish peroxidase
T5597 12425-12431 Protein denotes biotin
T5984 12536-12539 Protein denotes PKA
T5985 12572-12577 Protein denotes TNF-α
T5986 12578-12588 Gene_expression denotes production
T5987 12528-12532 Regulation denotes Role
T5988 12543-12554 Positive_regulation denotes LPS-induced
T5989 12613-12616 Protein denotes PKA
T5990 12620-12625 Protein denotes TNF-α
T5991 12712-12717 Protein denotes TNF-α
T5992 12779-12782 Protein denotes PKA
T5993 12626-12636 Gene_expression denotes production
T5994 12718-12728 Gene_expression denotes production
T5995 12783-12792 Negative_regulation denotes inhibitor
T5996 12605-12609 Regulation denotes role
T5997 12685-12696 Negative_regulation denotes attenuation
T5998 12666-12677 Regulation denotes involvement
T5999 13086-13089 Protein denotes PKA
T6000 13255-13258 Protein denotes PKA
T6001 13072-13082 Negative_regulation denotes inhibition
T6002 13259-13269 Negative_regulation denotes inhibitors
T6003 13304-13309 Protein denotes TNF-α
T6370 13870-13873 Protein denotes PTX
T6371 13889-13894 Protein denotes TNF-α
T6372 13895-13905 Gene_expression denotes production
T6373 13859-13866 Regulation denotes effects
T6374 13877-13888 Positive_regulation denotes LPS-induced
T6375 13935-13938 Protein denotes PDE
T6376 13953-13958 Protein denotes TNF-α
T6377 13939-13949 Negative_regulation denotes inhibition
T6378 13959-13969 Gene_expression denotes production
T6379 14060-14063 Protein denotes PTX
T6380 14100-14105 Protein denotes TNF-α
T6381 14106-14116 Gene_expression denotes production
T6382 14090-14096 Regulation denotes effect
T6950 14165-14170 Protein denotes NF-κB
T6951 14247-14252 Protein denotes I-κBα
T6952 14293-14298 Protein denotes NF-κB
T6953 14340-14345 Protein denotes I-κBα
T6954 14232-14243 Protein_catabolism denotes degradation
T6955 14268-14275 Entity denotes nuclear
T6956 14276-14289 Localization denotes translocation
T6957 14276-14289 Localization denotes translocation
T6958 14322-14329 Regulation denotes effects
T6959 14347-14360 Protein denotes Cytoplasmic I
T6960 14361-14364 Protein denotes κBα
T6961 14365-14380 Phosphorylation denotes phosphorylation
T6962 14365-14380 Phosphorylation denotes phosphorylation
T6963 14394-14403 Positive_regulation denotes increased
T6964 14394-14403 Positive_regulation denotes increased
T6965 14504-14507 Protein denotes PTX
T6966 14548-14553 Protein denotes I-κBα
T6967 14554-14569 Phosphorylation denotes phosphorylation
T6968 14522-14535 Negative_regulation denotes downregulated
T6969 14536-14547 Positive_regulation denotes LPS-induced
T6970 14522-14535 Negative_regulation denotes downregulated
T6971 14627-14630 Protein denotes p65
T6972 14613-14620 Entity denotes nuclear
T6973 14631-14646 Phosphorylation denotes phosphorylation
T6974 14651-14660 Positive_regulation denotes increased
T6975 14756-14761 Protein denotes NF-κB
T6976 14762-14765 Protein denotes p65
T6977 14803-14808 Protein denotes NF-κB
T6978 14766-14773 Entity denotes nuclear
T6979 14774-14789 Phosphorylation denotes phosphorylation
T6980 14774-14789 Phosphorylation denotes phosphorylation
T6981 14809-14819 Positive_regulation denotes activation
T6982 14832-14845 Localization denotes translocation
T6983 14832-14845 Localization denotes translocation
T6984 14744-14755 Positive_regulation denotes LPS-induced
T6985 14744-14755 Positive_regulation denotes LPS-induced
T6986 14734-14743 Negative_regulation denotes decreased
T6987 14734-14743 Negative_regulation denotes decreased
T6988 14928-14931 Protein denotes PTX
T6989 15038-15043 Protein denotes NF-κB
T6990 15018-15025 Binding denotes binding
T6991 15061-15064 Protein denotes PTX
T6992 15206-15211 Protein denotes NF-κB
T6993 15212-15227 Phosphorylation denotes phosphorylation
T6994 15188-15202 Negative_regulation denotes downregulation
T7503 15258-15262 Protein denotes CREB
T7504 15263-15278 Phosphorylation denotes phosphorylation
T7505 15283-15293 Positive_regulation denotes activation
T7506 15246-15257 Positive_regulation denotes upregulates
T7507 15373-15377 Protein denotes CREB
T7508 15378-15388 Positive_regulation denotes activation
T7509 15438-15442 Protein denotes CREB
T7510 15443-15458 Phosphorylation denotes phosphorylation
T7511 15426-15434 Positive_regulation denotes increase
T7512 15497-15500 Protein denotes PTX
T7513 15535-15539 Protein denotes CREB
T7514 15540-15555 Phosphorylation denotes phosphorylation
T7515 15523-15531 Positive_regulation denotes increase
T7516 15590-15593 Protein denotes PTX
T7517 15628-15632 Protein denotes CREB
T7518 15633-15648 Phosphorylation denotes phosphorylation
T7519 15667-15673 Positive_regulation denotes higher
T7520 15767-15771 Protein denotes CREB
T7521 15861-15864 Protein denotes PTX
T7522 15772-15787 Phosphorylation denotes phosphorylation
T7523 15959-15967 Protein denotes CREB-DNA
T7524 15992-15995 Protein denotes PTX
T7525 16019-16023 Protein denotes CREB
T7526 15968-15975 Binding denotes binding
T7527 15980-15988 Regulation denotes affected
T7528 16024-16039 Phosphorylation denotes phosphorylation
T7529 16100-16103 Protein denotes PTX
T7530 16124-16128 Protein denotes CREB
T7531 16114-16123 Positive_regulation denotes increased
T7977 16187-16190 Protein denotes PTX
T7978 16194-16199 Protein denotes TNF-α
T7979 16230-16233 Protein denotes PKA
T7980 16200-16210 Gene_expression denotes production
T7981 16234-16244 Negative_regulation denotes inhibition
T7982 16177-16183 Regulation denotes Effect
T7983 16257-16260 Protein denotes PKA
T7984 16264-16267 Protein denotes PTX
T7985 16294-16299 Protein denotes TNF-α
T7986 16402-16405 Protein denotes PKA
T7987 16434-16437 Protein denotes PTX
T7988 16300-16310 Gene_expression denotes production
T7989 16406-16415 Negative_regulation denotes inhibitor
T7990 16516-16521 Protein denotes TNF-α
T7991 16503-16512 Negative_regulation denotes reduction
T7992 16673-16676 Protein denotes PTX
T7993 16734-16739 Protein denotes TNF-α
T7994 16791-16794 Protein denotes PTX
T7995 16740-16750 Gene_expression denotes production
T7996 16720-16730 Regulation denotes modulation
T7997 16867-16872 Protein denotes TNF-α
T7998 16887-16890 Protein denotes PTX
T7999 16949-16952 Protein denotes PKA
T8000 16873-16883 Gene_expression denotes production
T8001 16849-16863 Negative_regulation denotes downregulation
T8002 16953-16964 Negative_regulation denotes independent
T10109 17198-17203 Protein denotes TNF-α
T10110 17438-17443 Protein denotes TNF-α
T10111 17151-17161 Gene_expression denotes production
T10112 17207-17215 Localization denotes released
T10113 17444-17453 Gene_expression denotes synthesis
T10114 17428-17437 Positive_regulation denotes increased
T10115 17684-17689 Protein denotes TNF-α
T10116 17673-17683 Negative_regulation denotes decreasing
T10117 17716-17726 Gene_expression denotes production
T10118 17700-17711 Regulation denotes attenuating
T10119 17744-17747 Protein denotes PDE
T10120 17791-17796 Protein denotes TNF-α
T10121 17748-17758 Negative_regulation denotes inhibitors
T10122 17797-17807 Gene_expression denotes production
T10123 17778-17790 Negative_regulation denotes downregulate
T10124 17887-17892 Protein denotes TNF-α
T10125 18024-18029 Protein denotes TNF-α
T10126 18120-18125 Protein denotes TNF-α
T10127 17893-17899 Gene_expression denotes levels
T10128 17912-17920 Positive_regulation denotes increase
T10129 18126-18136 Gene_expression denotes expression
T10130 18093-18104 Negative_regulation denotes attenuation
T10131 18221-18224 Protein denotes PTX
T10132 18311-18316 Protein denotes TNF-α
T10133 18317-18327 Gene_expression denotes production
T10134 18301-18310 Negative_regulation denotes decreases
T10135 18496-18499 Protein denotes PKA
T10136 18482-18492 Positive_regulation denotes activation
T10137 18669-18672 Protein denotes PKA
T10138 18789-18794 Protein denotes TNF-α
T10139 18893-18896 Protein denotes PKA
T10140 18795-18805 Gene_expression denotes production
T10141 18897-18907 Positive_regulation denotes activation
T10142 18774-18785 Negative_regulation denotes attenuation
T10143 18960-18963 Protein denotes PTX
T10144 19188-19191 Protein denotes PTX
T10145 19316-19321 Protein denotes TNF-α
T10146 19322-19331 Gene_expression denotes synthesis
T10147 19305-19312 Regulation denotes effects
T10148 19377-19380 Protein denotes PTX
T10149 19427-19435 Protein denotes TNF-αbut
T10150 19444-19447 Protein denotes PTX
T10151 19673-19676 Protein denotes PTX
T10152 19749-19754 Protein denotes NF-κB
T10153 19759-19763 Protein denotes CREB
T10154 19409-19423 Negative_regulation denotes downregulation
T10155 19835-19840 Protein denotes NF-κB
T10156 19841-19844 Protein denotes Rel
T10157 20025-20030 Protein denotes NF-κB
T10158 20038-20041 Protein denotes p50
T10159 20042-20045 Protein denotes p65
T10160 20082-20087 Protein denotes NF-κB
T10161 20154-20159 Protein denotes I-κBα
T10162 20112-20117 Binding denotes bound
T10163 20226-20231 Protein denotes NF-κB
T10164 20282-20293 Protein denotes I-κB kinase
T10165 20295-20298 Protein denotes IKK
T10166 20322-20327 Protein denotes IKK-1
T10167 20332-20337 Protein denotes IKK-2
T10168 20372-20385 Protein denotes I-κBα subunit
T10169 20232-20241 Positive_regulation denotes inducible
T10170 20249-20263 Phosphorylation denotes phosphorylates
T10171 20249-20263 Positive_regulation denotes phosphorylates
T10172 20300-20307 Entity denotes complex
T10173 20353-20367 Phosphorylation denotes phosphorylates
T10174 20353-20367 Phosphorylation denotes phosphorylates
T10175 20353-20367 Phosphorylation denotes phosphorylates
T10176 20268-20277 Positive_regulation denotes activates
T10177 20268-20277 Positive_regulation denotes activates
T10178 20268-20277 Positive_regulation denotes activates
T10179 20450-20455 Protein denotes I-κBα
T10180 20467-20472 Protein denotes NF-κB
T10181 20387-20402 Phosphorylation denotes Phosphorylation
T10182 20435-20446 Protein_catabolism denotes degradation
T10183 20473-20480 Entity denotes nuclear
T10184 20481-20494 Localization denotes translocation
T10185 20660-20668 Protein denotes κB sites
T10186 20716-20726 Protein denotes TNF-α gene
T10187 20728-20733 Protein denotes NF-κB
T10188 20800-20810 Protein denotes TNF-α gene
T10189 20536-20540 Binding denotes bind
T10190 20811-20821 Gene_expression denotes expression
T10191 20788-20796 Regulation denotes involved
T10192 20931-20934 Protein denotes κBα
T10193 20966-20969 Protein denotes p65
T10194 21012-21017 Protein denotes NF-κB
T10195 21069-21072 Protein denotes PTX
T10196 21124-21129 Protein denotes I-κBα
T10197 20935-20950 Phosphorylation denotes phosphorylation
T10198 20952-20959 Entity denotes nuclear
T10199 20970-20999 Phosphorylation denotes phosphorylation/translocation
T10200 21018-21029 Binding denotes DNA-binding
T10201 21130-21145 Phosphorylation denotes phosphorylation
T10202 20903-20916 Negative_regulation denotes downregulates
T10203 20903-20916 Negative_regulation denotes downregulates
T10204 20903-20916 Negative_regulation denotes downregulates
T10205 21115-21120 Positive_regulation denotes level
T10206 21223-21228 Protein denotes I-κBα
T10207 21233-21238 Protein denotes NF-κB
T10208 21352-21364 Protein denotes PTX on I-κBα
T10209 21369-21374 Protein denotes NF-κB
T10210 21513-21518 Protein denotes I-κBα
T10211 21523-21528 Protein denotes NF-κB
T10212 21549-21554 Protein denotes NF-κB
T10213 21623-21628 Protein denotes I-κBα
T10214 21493-21504 Binding denotes interaction
T10215 21540-21548 Negative_regulation denotes reducing
T10216 21629-21640 Protein_catabolism denotes degradation
T10217 21609-21619 Regulation denotes difference
T10218 21834-21839 Protein denotes NF-κB
T10219 21849-21853 Protein denotes CREB
T10220 21904-21907 Protein denotes p65
T10221 22134-22137 Protein denotes PKA
T10222 21798-21805 Negative_regulation denotes inhibit
T10223 21892-21900 Negative_regulation denotes decrease
T10224 22316-22319 Protein denotes PTX
T10225 22364-22369 Protein denotes NF-κB
T10226 22393-22396 Protein denotes PKA
T10227 22455-22460 Protein denotes TNF-α
T10228 22475-22478 Protein denotes PTX
T10229 22509-22512 Protein denotes PKA
T10230 22461-22471 Gene_expression denotes production
T10231 22461-22471 Gene_expression denotes production
T10232 22513-22523 Negative_regulation denotes inhibition
T10233 22443-22454 Positive_regulation denotes LPS-induced
T10234 22443-22454 Positive_regulation denotes LPS-induced
T10235 22428-22439 Negative_regulation denotes attenuation
T10236 22428-22439 Negative_regulation denotes attenuation
T10237 22649-22652 Protein denotes PKA
T10238 22762-22765 Protein denotes PKA
T10239 22653-22662 Negative_regulation denotes inhibitor
T10240 22844-22847 Protein denotes PTX
T10241 22884-22887 Protein denotes PDE
T10242 22921-22926 Protein denotes NF-κB
T10243 22991-22995 Protein denotes CREB
T10244 23000-23005 Protein denotes NF-κB
T10245 22888-22898 Negative_regulation denotes inhibitors
T10246 22980-22987 Binding denotes binding
T10247 22980-22987 Binding denotes binding
T10248 22955-22963 Positive_regulation denotes altering
T10249 22955-22963 Positive_regulation denotes altering
T10250 23123-23127 Protein denotes CREB
T10251 23084-23094 Positive_regulation denotes activation
T10252 23103-23110 Binding denotes binding
T10253 23131-23142 Positive_regulation denotes upregulated
T10254 23084-23094 Positive_regulation denotes activation
T10255 23203-23207 Protein denotes CREB
T10256 23296-23299 Protein denotes PTX
T10257 23308-23312 Protein denotes CREB
T10258 23370-23375 Protein denotes NF-κB
T10259 23191-23199 Positive_regulation denotes increase
T10260 23390-23401 Positive_regulation denotes upregulated
T10261 23503-23506 Protein denotes CBP
T10262 23525-23531 Protein denotes p30034
T10263 23626-23629 Protein denotes PTX
T10264 23471-23482 Binding denotes recruitment
T10265 23772-23776 Protein denotes AP-1
T10266 23778-23783 Protein denotes c-fos
T10267 23717-23724 Regulation denotes effects
T10268 23717-23724 Regulation denotes effects
T10269 23850-23854 Protein denotes AP-1
T10270 23877-23880 Protein denotes fos
T10271 23885-23890 Protein denotes c-jun
T10272 23964-23969 Protein denotes TNF-α
T10273 23836-23846 Positive_regulation denotes Activation
T10274 23836-23846 Positive_regulation denotes Activation
T10275 23836-23846 Positive_regulation denotes Activation
T10276 23970-23980 Gene_expression denotes expression
T10277 23992-23996 Protein denotes AP-1
T10278 24001-24005 Protein denotes CREB
T10279 24077-24089 Protein denotes TNF-α gene35
T10280 24006-24015 Binding denotes recognize
T10281 24141-24144 Protein denotes PTX
T10282 24206-24210 Protein denotes AP-1
T10283 24235-24239 Protein denotes CREB
T10284 24265-24275 Protein denotes TNF-α gene
T10285 24211-24221 Positive_regulation denotes activation
T10286 24240-24247 Binding denotes binding
T10287 24276-24289 Transcription denotes transcription
T10288 24196-24205 Regulation denotes affecting
T10289 24226-24234 Positive_regulation denotes favoring
T10290 24254-24264 Negative_regulation denotes inhibiting
T10291 24370-24374 Protein denotes CREB
T10292 24376-24381 Protein denotes NF-κB
T10293 24387-24391 Protein denotes AP-1
T10294 24462-24465 Protein denotes PTX
T10295 24610-24613 Protein denotes PDE
T10296 24666-24671 Protein denotes TNF-α
T10297 24712-24715 Protein denotes PKA
T10298 24614-24624 Negative_regulation denotes inhibition
T10299 24672-24682 Gene_expression denotes production
T10300 24652-24665 Negative_regulation denotes downregulates
T10301 24752-24755 Protein denotes PTX
T10302 24783-24787 Protein denotes NF-B
T10303 24807-24811 Protein denotes CREB
T10304 24812-24822 Positive_regulation denotes activation
T10305 24794-24806 Positive_regulation denotes upregulating
T10306 24928-24931 Protein denotes PTX
T10307 25018-25023 Protein denotes TNF-α
T10308 25024-25034 Gene_expression denotes production
R665 T912 T915 themeOf TNF-α,Production
R666 T914 T916 themeOf Specific Phosphodiesterase,Inhibition
R667 T915 T917 themeOf Production,Regulation
R668 T916 T918 themeOf Inhibition,Effects
R669 T919 T921 themeOf tumor necrosis factor-alpha,production
R670 T920 T922 themeOf TNF-α,production
R671 T921 T923 themeOf production,activation
R672 T922 T924 themeOf production,activation
R673 T923 T925 themeOf activation,effect
R674 T924 T926 themeOf activation,effect
R675 T927 T928 themeOf TNF-α,production
R676 T928 T929 themeOf production,stimulation
R677 T930 T931 themeOf TNF-α,production
R678 T931 T932 themeOf production,alters
R679 T939 T941 themeOf NF-κB,binding
R680 T940 T942 themeOf CREB,binding
R681 T944 T945 themeOf Protein kinase A,inhibition
R682 T946 T956 causeOf PTX,reduce
R683 T947 T951 themeOf cytoplasmic I,phosphorylation
R684 T948 T952 themeOf κBα,phosphorylation
R685 T949 T954 themeOf p65,phosphorylation
R686 T950 T955 themeOf NF-κB,binding
R687 T953 T954 Site nuclear,phosphorylation
R688 T954 T956 themeOf phosphorylation,reduce
R689 T957 T960 causeOf PTX,enhanced
R690 T958 T959 themeOf CREB,phosphorylation
R691 T959 T960 themeOf phosphorylation,enhanced
R692 T961 T964 themeOf PTX,secreted
R693 T962 T965 themeOf TNF-α,secreted
R694 T963 T966 themeOf Protein kinase A,inhibition
R695 T967 T969 themeOf NF-κB,binding
R696 T969 T970 themeOf binding,activation
R1748 T2232 T2233 themeOf multiple host intracellular protein kinases and transcription factors,phosphorylation
R1749 T2233 T2234 themeOf phosphorylation,activation
R1750 T2233 T2235 themeOf phosphorylation,initiating
R1751 T2234 T2236 themeOf activation,initiating
R1752 T2237 T2240 themeOf tumor necrosis factor-α,regulator
R1753 T2238 T2241 themeOf TNF-α,regulator
R1754 T2242 T2246 themeOf TNF-α,synthesis
R1755 T2243 T2248 themeOf PDE,inhibition
R1756 T2244 T2250 themeOf TNF-α,production
R1757 T2245 T2249 themeOf phosphodiesterase,inhibition
R1758 T2246 T2247 themeOf synthesis,attenuated
R1759 T2250 T2251 themeOf production,downregulation
R1760 T2280 T2281 themeOf production,down-regulates
R1761 T2252 T2257 themeOf Protein kinase A,accumulation
R1762 T2282 T2289 themeOf NF-κB,binding
R1763 T2253 T2259 themeOf PKA,activation
R1764 T2284 T2290 themeOf CREB,activation
R1765 T2285 T2291 themeOf NF-κB,competition
R1766 T2286 T2291 themeOf CREB,competition
R1767 T2289 T2292 themeOf binding,inhibition
R1768 T2254 T2260 themeOf PKA,activation
R1769 T2255 T2263 themeOf cAMP-response element binding protein,phosphorylation
R1770 T2293 T2294 themeOf PKA,activation
R1771 T2256 T2264 themeOf CREB,phosphorylation
R1772 T2295 T2297 themeOf NF-κB,activation
R1773 T2296 T2298 themeOf CREB,activation
R1774 T2257 T2258 themeOf accumulation,activation
R1775 T2258 T2265 themeOf activation,results
R1776 T2299 T2302 causeOf PKA,role
R1777 T2300 T2301 themeOf TNF-α,production
R1778 T2301 T2302 themeOf production,role
R1779 T2301 T2303 themeOf production,LPS-induced
R1780 T2259 T2261 themeOf activation,leads
R1781 T2260 T2262 themeOf activation,leads
R1782 T2267 T2269 themeOf transcription factor nuclear factor-κB,activation
R1783 T2268 T2270 themeOf NF-κB,activation
R1784 T2271 T2273 themeOf NF-κB,transcription
R1785 T2272 T2274 themeOf TNF-α,transcription
R1787 T2272 T2275 causeOf TNF-α,inhibit
R1788 T2272 T2276 causeOf TNF-α,inhibit
R1789 T2273 T2275 themeOf transcription,inhibit
R1791 T2274 T2276 themeOf transcription,inhibit
R1792 T2277 T2279 themeOf PDE,inhibition
R1794 T2278 T2280 themeOf TNF-α,production
R1796 T2279 T2281 causeOf inhibition,down-regulates
R2541 T3158 T3162 themeOf CREB,phosphorylated
R2542 T3160 T3163 themeOf NF-κB,phosphorylated
R2543 T3161 T3164 themeOf p65 subunit,phosphorylated
R2544 T3167 T3169 themeOf CREB,synthesized
R2545 T3168 T3170 themeOf NF-κB DNA oligonucleotide,synthesized
R3211 T3909 T3912 causeOf PTX,effects
R3212 T3910 T3911 themeOf TNF-α,production
R3213 T3911 T3912 themeOf production,effects
R3214 T3914 T3915 themeOf TNF-α,production
R3215 T3915 T3916 themeOf production,up-regulation
R3216 T3915 T3918 themeOf production,down-regulation
R3217 T3916 T3917 themeOf up-regulation,down-regulation
R3218 T3917 T3919 themeOf down-regulation,up-regulation
R3219 T3918 T3920 themeOf down-regulation,up-regulation
R3353 T4088 T4091 causeOf PTX,Effect
R3354 T4089 T4090 themeOf TNF-α,production
R3355 T4090 T4091 themeOf production,Effect
R3356 T4090 T4092 themeOf production,LPS-induced
R4021 T4853 T4856 themeOf I-κBα antibody,phosphorylated
R4022 T4854 T4857 themeOf NF-κB p65 antibody,phosphorylated
R4023 T4855 T4858 themeOf CREB,phosphorylated
R4980 T5984 T5987 causeOf PKA,Role
R4981 T5985 T5986 themeOf TNF-α,production
R4982 T5986 T5987 themeOf production,Role
R4983 T5986 T5988 themeOf production,LPS-induced
R4984 T5989 T5996 causeOf PKA,role
R4985 T5990 T5993 themeOf TNF-α,production
R4986 T5991 T5994 themeOf TNF-α,production
R4987 T5992 T5995 themeOf PKA,inhibitor
R4988 T5993 T5996 themeOf production,role
R4989 T5994 T5997 themeOf production,attenuation
R4990 T5997 T5998 themeOf attenuation,involvement
R4991 T5999 T6001 themeOf PKA,inhibition
R4992 T6000 T6002 themeOf PKA,inhibitors
R5292 T6370 T6373 causeOf PTX,effects
R5293 T6371 T6372 themeOf TNF-α,production
R5294 T6372 T6373 themeOf production,effects
R5295 T6372 T6374 themeOf production,LPS-induced
R5296 T6375 T6377 themeOf PDE,inhibition
R5297 T6376 T6378 themeOf TNF-α,production
R5298 T6379 T6382 causeOf PTX,effect
R5299 T6380 T6381 themeOf TNF-α,production
R5300 T6381 T6382 themeOf production,effect
R5782 T6951 T6954 themeOf I-κBα,degradation
R5783 T6951 T6956 themeOf I-κBα,translocation
R5784 T6952 T6957 themeOf NF-κB,translocation
R5785 T6953 T6958 themeOf I-κBα,effects
R5786 T6955 T6956 locationOf nuclear,translocation
R5787 T6955 T6957 locationOf nuclear,translocation
R5788 T6959 T6961 themeOf Cytoplasmic I,phosphorylation
R5789 T6960 T6962 themeOf κBα,phosphorylation
R5790 T6961 T6963 themeOf phosphorylation,increased
R5791 T6962 T6964 themeOf phosphorylation,increased
R5792 T6965 T6968 causeOf PTX,downregulated
R5793 T6965 T6970 causeOf PTX,downregulated
R5794 T6966 T6967 themeOf I-κBα,phosphorylation
R5795 T6967 T6968 themeOf phosphorylation,downregulated
R5796 T6967 T6969 themeOf phosphorylation,LPS-induced
R5797 T6969 T6970 themeOf LPS-induced,downregulated
R5798 T6971 T6973 themeOf p65,phosphorylation
R5799 T6972 T6973 Site nuclear,phosphorylation
R5800 T6973 T6974 themeOf phosphorylation,increased
R5801 T6975 T6979 themeOf NF-κB,phosphorylation
R5802 T6975 T6982 themeOf NF-κB,translocation
R5803 T6976 T6980 themeOf p65,phosphorylation
R5804 T6976 T6983 themeOf p65,translocation
R5805 T6977 T6981 themeOf NF-κB,activation
R5806 T6978 T6979 Site nuclear,phosphorylation
R5807 T6978 T6980 Site nuclear,phosphorylation
R5808 T6979 T6984 themeOf phosphorylation,LPS-induced
R5809 T6980 T6985 themeOf phosphorylation,LPS-induced
R5810 T6984 T6986 themeOf LPS-induced,decreased
R5811 T6985 T6987 themeOf LPS-induced,decreased
R5812 T6989 T6990 themeOf NF-κB,binding
R5813 T6992 T6993 themeOf NF-κB,phosphorylation
R5814 T6993 T6994 themeOf phosphorylation,downregulation
R6204 T7503 T7504 themeOf CREB,phosphorylation
R6205 T7504 T7505 themeOf phosphorylation,activation
R6206 T7505 T7506 themeOf activation,upregulates
R6207 T7507 T7508 themeOf CREB,activation
R6208 T7509 T7510 themeOf CREB,phosphorylation
R6209 T7510 T7511 themeOf phosphorylation,increase
R6210 T7512 T7515 causeOf PTX,increase
R6211 T7513 T7514 themeOf CREB,phosphorylation
R6212 T7514 T7515 themeOf phosphorylation,increase
R6213 T7517 T7518 themeOf CREB,phosphorylation
R6214 T7518 T7519 themeOf phosphorylation,higher
R6215 T7520 T7522 themeOf CREB,phosphorylation
R6216 T7523 T7526 themeOf CREB-DNA,binding
R6217 T7524 T7527 causeOf PTX,affected
R6218 T7525 T7528 themeOf CREB,phosphorylation
R6219 T7526 T7527 themeOf binding,affected
R6220 T7530 T7531 themeOf CREB,increased
R6586 T7977 T7982 causeOf PTX,Effect
R6587 T7978 T7980 themeOf TNF-α,production
R6588 T7979 T7981 themeOf PKA,inhibition
R6589 T7980 T7982 themeOf production,Effect
R6590 T7985 T7988 themeOf TNF-α,production
R6591 T7986 T7989 themeOf PKA,inhibitor
R6592 T7990 T7991 themeOf TNF-α,reduction
R6593 T7993 T7995 themeOf TNF-α,production
R6594 T7995 T7996 themeOf production,modulation
R6595 T7997 T8000 themeOf TNF-α,production
R6596 T7998 T8001 causeOf PTX,downregulation
R6597 T7999 T8002 causeOf PKA,independent
R6598 T8000 T8001 themeOf production,downregulation
R6599 T8001 T8002 themeOf downregulation,independent
R8201 T10109 T10111 themeOf TNF-α,production
R8202 T10109 T10112 themeOf TNF-α,released
R8203 T10110 T10113 themeOf TNF-α,synthesis
R8204 T10113 T10114 themeOf synthesis,increased
R8205 T10115 T10116 themeOf TNF-α,decreasing
R8206 T10115 T10117 themeOf TNF-α,production
R8207 T10117 T10118 themeOf production,attenuating
R8208 T10119 T10121 themeOf PDE,inhibitors
R8209 T10120 T10122 themeOf TNF-α,production
R8210 T10121 T10123 causeOf inhibitors,downregulate
R8211 T10122 T10123 themeOf production,downregulate
R8212 T10124 T10127 themeOf TNF-α,levels
R8213 T10126 T10129 themeOf TNF-α,expression
R8214 T10127 T10128 themeOf levels,increase
R8215 T10129 T10130 themeOf expression,attenuation
R8216 T10131 T10134 causeOf PTX,decreases
R8217 T10132 T10133 themeOf TNF-α,production
R8218 T10133 T10134 themeOf production,decreases
R8219 T10135 T10136 themeOf PKA,activation
R8220 T10138 T10140 themeOf TNF-α,production
R8221 T10139 T10141 themeOf PKA,activation
R8222 T10140 T10142 themeOf production,attenuation
R8223 T10227 T10230 themeOf TNF-α,production
R8224 T10145 T10146 themeOf TNF-α,synthesis
R8225 T10146 T10147 themeOf synthesis,effects
R8226 T10228 T10231 themeOf PTX,production
R8227 T10149 T10154 themeOf TNF-αbut,downregulation
R8228 T10229 T10232 themeOf PKA,inhibition
R8229 T10230 T10233 themeOf production,LPS-induced
R8230 T10161 T10162 themeOf I-κBα,bound
R8231 T10231 T10234 themeOf production,LPS-induced
R8232 T10163 T10169 themeOf NF-κB,inducible
R8233 T10163 T10170 themeOf NF-κB,phosphorylates
R8234 T10233 T10235 themeOf LPS-induced,attenuation
R8235 T10164 T10173 themeOf I-κB kinase,phosphorylates
R8236 T10164 T10172 partOf I-κB kinase,complex
R8237 T10165 T10174 themeOf IKK,phosphorylates
R8238 T10165 T10172 partOf IKK,complex
R8239 T10167 T10172 partOf IKK-2,complex
R8240 T10168 T10175 themeOf I-κBα subunit,phosphorylates
R8241 T10234 T10236 themeOf LPS-induced,attenuation
R8242 T10170 T10171 themeOf phosphorylates,phosphorylates
R8243 T10237 T10239 themeOf PKA,inhibitor
R8244 T10172 T10173 Site complex,phosphorylates
R8245 T10172 T10174 Site complex,phosphorylates
R8246 T10172 T10175 Site complex,phosphorylates
R8247 T10172 T10176 Site complex,activates
R8248 T10172 T10177 Site complex,activates
R8249 T10172 T10178 Site complex,activates
R8250 T10173 T10176 themeOf phosphorylates,activates
R8251 T10174 T10177 themeOf phosphorylates,activates
R8252 T10175 T10178 themeOf phosphorylates,activates
R8253 T10241 T10245 themeOf PDE,inhibitors
R8254 T10179 T10181 themeOf I-κBα,Phosphorylation
R8255 T10179 T10182 themeOf I-κBα,degradation
R8256 T10180 T10184 themeOf NF-κB,translocation
R8257 T10183 T10184 locationOf nuclear,translocation
R8258 T10243 T10246 themeOf CREB,binding
R8259 T10187 T10189 themeOf NF-κB,bind
R8260 T10188 T10190 themeOf TNF-α gene,expression
R8261 T10244 T10247 themeOf NF-κB,binding
R8262 T10190 T10191 themeOf expression,involved
R8263 T10192 T10197 themeOf κBα,phosphorylation
R8264 T10193 T10199 themeOf p65,phosphorylation/translocation
R8265 T10194 T10200 themeOf NF-κB,DNA-binding
R8266 T10246 T10248 themeOf binding,altering
R8267 T10196 T10201 themeOf I-κBα,phosphorylation
R8268 T10197 T10202 themeOf phosphorylation,downregulates
R8269 T10198 T10199 Site nuclear,phosphorylation/translocation
R8270 T10199 T10203 themeOf phosphorylation/translocation,downregulates
R8271 T10200 T10204 themeOf DNA-binding,downregulates
R8272 T10247 T10249 themeOf binding,altering
R8273 T10201 T10205 themeOf phosphorylation,level
R8274 T10250 T10251 themeOf CREB,activation
R8275 T10250 T10252 themeOf CREB,binding
R8276 T10210 T10214 themeOf I-κBα,interaction
R8277 T10211 T10214 themeOf NF-κB,interaction
R8278 T10213 T10216 themeOf I-κBα,degradation
R8279 T10214 T10215 themeOf interaction,reducing
R8280 T10251 T10253 themeOf activation,upregulated
R8281 T10216 T10217 themeOf degradation,difference
R8282 T10252 T10254 themeOf binding,activation
R8283 T10218 T10222 themeOf NF-κB,inhibit
R8284 T10220 T10223 themeOf p65,decrease
R8285 T10255 T10259 themeOf CREB,increase
R8286 T10258 T10260 themeOf NF-κB,upregulated
R8287 T10261 T10264 themeOf CBP,recruitment
R8288 T10262 T10264 themeOf p30034,recruitment
R8289 T10265 T10267 themeOf AP-1,effects
R8290 T10266 T10268 themeOf c-fos,effects
R8291 T10269 T10273 themeOf AP-1,Activation
R8292 T10270 T10274 themeOf fos,Activation
R8293 T10271 T10275 themeOf c-jun,Activation
R8295 T10272 T10276 themeOf TNF-α,expression
R8296 T10277 T10280 themeOf AP-1,recognize
R8299 T10281 T10288 causeOf PTX,affecting
R8301 T10281 T10290 causeOf PTX,inhibiting
R8302 T10282 T10285 themeOf AP-1,activation
R8304 T10283 T10286 themeOf CREB,binding
R8306 T10284 T10287 themeOf TNF-α gene,transcription
R8307 T10285 T10288 themeOf activation,affecting
R8309 T10286 T10289 themeOf binding,favoring
R8310 T10287 T10290 themeOf transcription,inhibiting
R8312 T10295 T10298 themeOf PDE,inhibition
R8315 T10296 T10299 themeOf TNF-α,production
R8316 T10298 T10300 causeOf inhibition,downregulates
R8318 T10299 T10300 themeOf production,downregulates
R8320 T10303 T10304 themeOf CREB,activation
R8323 T10304 T10305 themeOf activation,upregulating
R8324 T10307 T10308 themeOf TNF-α,production

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T757 32-35 P01375 denotes TNF
T758 32-37 P01375 denotes TNF-α
T759 294-315 P01375 denotes tumor necrosis factor
T760 323-326 P01375 denotes TNF
T761 323-328 P01375 denotes TNF-α
T762 437-440 P01375 denotes TNF
T763 437-442 P01375 denotes TNF-α
T764 590-593 P01375 denotes TNF
T765 590-595 P01375 denotes TNF-α
T766 891-894 P21579 denotes p65
T767 891-894 Q04206 denotes p65
T768 916-953 O43889 denotes cAMP-response element binding protein
T769 916-953 P15336 denotes cAMP-response element binding protein
T770 916-953 P18848 denotes cAMP-response element binding protein
T771 916-953 Q8TEY5 denotes cAMP-response element binding protein
T772 916-953 Q02930 denotes cAMP-response element binding protein
T773 916-953 P16220 denotes cAMP-response element binding protein
T774 955-959 P16220 denotes CREB
T775 955-959 O43889 denotes CREB
T776 955-959 Q8TEY5 denotes CREB
T777 955-959 Q02930 denotes CREB
T778 955-959 P18848 denotes CREB
T779 955-959 P15336 denotes CREB
T780 1001-1005 O43889 denotes CREB
T781 1001-1005 Q02930 denotes CREB
T782 1001-1005 P15336 denotes CREB
T783 1001-1005 P16220 denotes CREB
T784 1001-1005 Q8TEY5 denotes CREB
T785 1001-1005 P18848 denotes CREB
T786 1060-1063 P01375 denotes TNF
T787 1060-1065 P01375 denotes TNF-α
T788 1330-1333 Q04206 denotes p65
T789 1330-1333 P21579 denotes p65
T790 1473-1477 O43889 denotes CREB
T791 1473-1477 Q8TEY5 denotes CREB
T792 1473-1477 Q02930 denotes CREB
T793 1473-1477 P18848 denotes CREB
T794 1473-1477 P16220 denotes CREB
T795 1473-1477 P15336 denotes CREB
T796 1551-1554 P01375 denotes TNF
T797 1551-1556 P01375 denotes TNF-α
T798 1801-1805 P16220 denotes CREB
T799 1801-1805 P18848 denotes CREB
T800 1801-1805 Q02930 denotes CREB
T801 1801-1805 P15336 denotes CREB
T802 1801-1805 Q8TEY5 denotes CREB
T803 1801-1805 O43889 denotes CREB
T2041 2277-2320 Q02548 denotes transcription factors. Transcription factor
T2042 2277-2320 Q9UL17 denotes transcription factors. Transcription factor
T2043 2571-2592 P01375 denotes tumor necrosis factor
T2044 2596-2599 P01375 denotes TNF
T2045 2596-2601 P01375 denotes TNF-α
T2046 2616-2619 P01375 denotes TNF
T2047 2616-2621 P01375 denotes TNF-α
T2048 2724-2727 P01375 denotes TNF
T2049 2724-2729 P01375 denotes TNF-α
T2050 2982-2985 P01375 denotes TNF
T2051 2982-2987 P01375 denotes TNF-α
T2052 3353-3390 P15336 denotes cAMP-response element binding protein
T2053 3353-3390 Q02930 denotes cAMP-response element binding protein
T2054 3353-3390 P16220 denotes cAMP-response element binding protein
T2055 3353-3390 O43889 denotes cAMP-response element binding protein
T2056 3353-3390 P18848 denotes cAMP-response element binding protein
T2057 3353-3390 Q8TEY5 denotes cAMP-response element binding protein
T2058 3392-3396 Q8TEY5 denotes CREB
T2059 3392-3396 P15336 denotes CREB
T2060 3392-3396 P16220 denotes CREB
T2061 3392-3396 P18848 denotes CREB
T2062 3392-3396 Q02930 denotes CREB
T2063 3392-3396 O43889 denotes CREB
T2064 4107-4110 P01375 denotes TNF
T2065 4107-4112 P01375 denotes TNF-α
T2066 4211-4214 P01375 denotes TNF
T2067 4211-4216 P01375 denotes TNF-α
T2068 4378-4382 O43889 denotes CREB
T2069 4378-4382 P15336 denotes CREB
T2070 4378-4382 P16220 denotes CREB
T2071 4378-4382 Q8TEY5 denotes CREB
T2072 4378-4382 P18848 denotes CREB
T2073 4378-4382 Q02930 denotes CREB
T2074 4429-4433 O43889 denotes CREB
T2075 4429-4433 Q8TEY5 denotes CREB
T2076 4429-4433 P15336 denotes CREB
T2077 4429-4433 P16220 denotes CREB
T2078 4429-4433 P18848 denotes CREB
T2079 4429-4433 Q02930 denotes CREB
T2080 4467-4471 P16220 denotes CREB
T2081 4467-4471 P15336 denotes CREB
T2082 4467-4471 P18848 denotes CREB
T2083 4467-4471 Q8TEY5 denotes CREB
T2084 4467-4471 O43889 denotes CREB
T2085 4467-4471 Q02930 denotes CREB
T2086 4467-4487 Q92793 denotes CREB binding protein
T2087 4489-4492 Q92793 denotes CBP
T2088 4760-4764 P15336 denotes CREB
T2089 4760-4764 Q02930 denotes CREB
T2090 4760-4764 P16220 denotes CREB
T2091 4760-4764 Q8TEY5 denotes CREB
T2092 4760-4764 P18848 denotes CREB
T2093 4760-4764 O43889 denotes CREB
T2094 4902-4905 P01375 denotes TNF
T2095 4902-4907 P01375 denotes TNF-α
T3100 5682-5686 Q8TEY5 denotes CREB
T3101 5682-5686 Q02930 denotes CREB
T3102 5682-5686 P18848 denotes CREB
T3103 5682-5686 P16220 denotes CREB
T3104 5682-5686 O43889 denotes CREB
T3105 5682-5686 P15336 denotes CREB
T3106 5764-5767 Q04206 denotes p65
T3107 5764-5767 P21579 denotes p65
T3108 6012-6015 P01375 denotes TNF
T3109 6012-6017 P01375 denotes TNF-α
T3110 6440-6444 P15336 denotes CREB
T3111 6440-6444 P18848 denotes CREB
T3112 6440-6444 P16220 denotes CREB
T3113 6440-6444 Q02930 denotes CREB
T3114 6440-6444 Q8TEY5 denotes CREB
T3115 6440-6444 O43889 denotes CREB
T3868 8225-8228 P01375 denotes TNF
T3869 8225-8230 P01375 denotes TNF-α
T3870 8431-8434 P01375 denotes TNF
T3871 8431-8436 P01375 denotes TNF-α
T4073 8534-8537 P01375 denotes TNF
T4074 8534-8539 P01375 denotes TNF-α
T4075 8593-8596 P01375 denotes TNF
T4076 8593-8598 P01375 denotes TNF-α
T4790 8853-8856 Q04206 denotes p65
T4791 8853-8856 P21579 denotes p65
T4792 8862-8866 P15336 denotes CREB
T4793 8862-8866 P16220 denotes CREB
T4794 8862-8866 P18848 denotes CREB
T4795 8862-8866 O43889 denotes CREB
T4796 8862-8866 Q02930 denotes CREB
T4797 8862-8866 Q8TEY5 denotes CREB
T4798 9939-9942 Q04206 denotes p65
T4799 9939-9942 P21579 denotes p65
T4800 9978-9982 P15336 denotes CREB
T4801 9978-9982 P18848 denotes CREB
T4802 9978-9982 P16220 denotes CREB
T4803 9978-9982 Q02930 denotes CREB
T4804 9978-9982 Q8TEY5 denotes CREB
T4805 9978-9982 O43889 denotes CREB
T5533 10812-10816 O43889 denotes CREB
T5534 10812-10816 Q8TEY5 denotes CREB
T5535 10812-10816 Q02930 denotes CREB
T5536 10812-10816 P18848 denotes CREB
T5537 10812-10816 P16220 denotes CREB
T5538 10812-10816 P15336 denotes CREB
T5539 11347-11351 O43889 denotes CREB
T5540 11347-11351 Q8TEY5 denotes CREB
T5541 11347-11351 Q02930 denotes CREB
T5542 11347-11351 P18848 denotes CREB
T5543 11347-11351 P15336 denotes CREB
T5544 11347-11351 P16220 denotes CREB
T5545 11347-11353 O43889 denotes CREB 3
T5546 11514-11517 P06956 denotes CRE
T5547 11668-11672 Q02930 denotes CREB
T5548 11668-11672 P18848 denotes CREB
T5549 11668-11672 Q8TEY5 denotes CREB
T5550 11668-11672 P15336 denotes CREB
T5551 11668-11672 P16220 denotes CREB
T5552 11668-11672 O43889 denotes CREB
T5553 11749-11753 Q02930 denotes CREB
T5554 11749-11753 P18848 denotes CREB
T5555 11749-11753 P16220 denotes CREB
T5556 11749-11753 P15336 denotes CREB
T5557 11749-11753 O43889 denotes CREB
T5558 11749-11753 Q8TEY5 denotes CREB
T5559 11869-11873 Q8TEY5 denotes CREB
T5560 11869-11873 P18848 denotes CREB
T5561 11869-11873 P16220 denotes CREB
T5562 11869-11873 P15336 denotes CREB
T5563 11869-11873 Q02930 denotes CREB
T5564 11869-11873 O43889 denotes CREB
T5565 11906-11908 P0A7Z4 denotes pH
T5942 12572-12575 P01375 denotes TNF
T5943 12572-12577 P01375 denotes TNF-α
T5944 12620-12623 P01375 denotes TNF
T5945 12620-12625 P01375 denotes TNF-α
T5946 12712-12715 P01375 denotes TNF
T5947 12712-12717 P01375 denotes TNF-α
T5948 13304-13307 P01375 denotes TNF
T5949 13304-13309 P01375 denotes TNF-α
T6342 13889-13892 P01375 denotes TNF
T6343 13889-13894 P01375 denotes TNF-α
T6344 13953-13956 P01375 denotes TNF
T6345 13953-13958 P01375 denotes TNF-α
T6346 14100-14103 P01375 denotes TNF
T6347 14100-14105 P01375 denotes TNF-α
T6880 14627-14630 Q04206 denotes p65
T6881 14627-14630 P21579 denotes p65
T6882 14762-14765 Q04206 denotes p65
T6883 14762-14765 P21579 denotes p65
T7418 15258-15262 Q8TEY5 denotes CREB
T7419 15258-15262 Q02930 denotes CREB
T7420 15258-15262 P18848 denotes CREB
T7421 15258-15262 P16220 denotes CREB
T7422 15258-15262 P15336 denotes CREB
T7423 15258-15262 O43889 denotes CREB
T7424 15343-15347 Q8TEY5 denotes CREB
T7425 15343-15347 Q02930 denotes CREB
T7426 15343-15347 P18848 denotes CREB
T7427 15343-15347 P16220 denotes CREB
T7428 15343-15347 P15336 denotes CREB
T7429 15343-15347 O43889 denotes CREB
T7430 15373-15377 Q8TEY5 denotes CREB
T7431 15373-15377 Q02930 denotes CREB
T7432 15373-15377 P18848 denotes CREB
T7433 15373-15377 P16220 denotes CREB
T7434 15373-15377 P15336 denotes CREB
T7435 15373-15377 O43889 denotes CREB
T7436 15438-15442 Q8TEY5 denotes CREB
T7437 15438-15442 Q02930 denotes CREB
T7438 15438-15442 P18848 denotes CREB
T7439 15438-15442 P16220 denotes CREB
T7440 15438-15442 P15336 denotes CREB
T7441 15438-15442 O43889 denotes CREB
T7442 15535-15539 Q8TEY5 denotes CREB
T7443 15535-15539 Q02930 denotes CREB
T7444 15535-15539 P18848 denotes CREB
T7445 15535-15539 P16220 denotes CREB
T7446 15535-15539 P15336 denotes CREB
T7447 15535-15539 O43889 denotes CREB
T7448 15628-15632 Q8TEY5 denotes CREB
T7449 15628-15632 Q02930 denotes CREB
T7450 15628-15632 P18848 denotes CREB
T7451 15628-15632 P16220 denotes CREB
T7452 15628-15632 P15336 denotes CREB
T7453 15628-15632 O43889 denotes CREB
T7454 15767-15771 Q8TEY5 denotes CREB
T7455 15767-15771 Q02930 denotes CREB
T7456 15767-15771 P18848 denotes CREB
T7457 15767-15771 P16220 denotes CREB
T7458 15767-15771 P15336 denotes CREB
T7459 15767-15771 O43889 denotes CREB
T7460 15959-15963 Q8TEY5 denotes CREB
T7461 15959-15963 Q02930 denotes CREB
T7462 15959-15963 P18848 denotes CREB
T7463 15959-15963 P16220 denotes CREB
T7464 15959-15963 P15336 denotes CREB
T7465 15959-15963 O43889 denotes CREB
T7466 16019-16023 Q8TEY5 denotes CREB
T7467 16019-16023 Q02930 denotes CREB
T7468 16019-16023 P18848 denotes CREB
T7469 16019-16023 P16220 denotes CREB
T7470 16019-16023 P15336 denotes CREB
T7471 16019-16023 O43889 denotes CREB
T7472 16124-16128 Q8TEY5 denotes CREB
T7473 16124-16128 Q02930 denotes CREB
T7474 16124-16128 P18848 denotes CREB
T7475 16124-16128 P16220 denotes CREB
T7476 16124-16128 P15336 denotes CREB
T7477 16124-16128 O43889 denotes CREB
T7895 16194-16197 P01375 denotes TNF
T7896 16194-16199 P01375 denotes TNF-α
T7897 16294-16297 P01375 denotes TNF
T7898 16294-16299 P01375 denotes TNF-α
T7899 16516-16519 P01375 denotes TNF
T7900 16516-16521 P01375 denotes TNF-α
T7901 16734-16737 P01375 denotes TNF
T7902 16734-16739 P01375 denotes TNF-α
T7903 16867-16870 P01375 denotes TNF
T7904 16867-16872 P01375 denotes TNF-α
T9704 17198-17201 P01375 denotes TNF
T9705 17198-17203 P01375 denotes TNF-α
T9706 17438-17441 P01375 denotes TNF
T9707 17438-17443 P01375 denotes TNF-α
T9708 17664-17669 P26358 denotes aimed
T9709 17684-17687 P01375 denotes TNF
T9710 17684-17689 P01375 denotes TNF-α
T9711 17791-17794 P01375 denotes TNF
T9712 17791-17796 P01375 denotes TNF-α
T9713 17887-17890 P01375 denotes TNF
T9714 17887-17892 P01375 denotes TNF-α
T9715 18024-18027 P01375 denotes TNF
T9716 18024-18029 P01375 denotes TNF-α
T9717 18120-18123 P01375 denotes TNF
T9718 18120-18125 P01375 denotes TNF-α
T9719 18311-18314 P01375 denotes TNF
T9720 18311-18316 P01375 denotes TNF-α
T9721 18789-18792 P01375 denotes TNF
T9722 18789-18794 P01375 denotes TNF-α
T9723 19316-19319 P01375 denotes TNF
T9724 19316-19321 P01375 denotes TNF-α
T9725 19427-19430 P01375 denotes TNF
T9726 19759-19763 P18848 denotes CREB
T9727 19759-19763 Q8TEY5 denotes CREB
T9728 19759-19763 Q02930 denotes CREB
T9729 19759-19763 P15336 denotes CREB
T9730 19759-19763 O43889 denotes CREB
T9731 19759-19763 P16220 denotes CREB
T9732 19841-19844 Q04864 denotes Rel
T9733 20038-20041 P19838 denotes p50
T9734 20042-20045 Q04206 denotes p65
T9735 20042-20045 P21579 denotes p65
T9736 20716-20719 P01375 denotes TNF
T9737 20716-20721 P01375 denotes TNF-α
T9738 20800-20803 P01375 denotes TNF
T9739 20800-20805 P01375 denotes TNF-α
T9740 20966-20969 Q04206 denotes p65
T9741 20966-20969 P21579 denotes p65
T9742 21849-21853 Q8TEY5 denotes CREB
T9743 21849-21853 Q02930 denotes CREB
T9744 21849-21853 P18848 denotes CREB
T9745 21849-21853 P16220 denotes CREB
T9746 21849-21853 P15336 denotes CREB
T9747 21849-21853 O43889 denotes CREB
T9748 21904-21907 P21579 denotes p65
T9749 21904-21907 Q04206 denotes p65
T9750 22455-22458 P01375 denotes TNF
T9751 22455-22460 P01375 denotes TNF-α
T9752 22991-22995 P15336 denotes CREB
T9753 22991-22995 P16220 denotes CREB
T9754 22991-22995 O43889 denotes CREB
T9755 22991-22995 P18848 denotes CREB
T9756 22991-22995 Q02930 denotes CREB
T9757 22991-22995 Q8TEY5 denotes CREB
T9758 23123-23127 Q8TEY5 denotes CREB
T9759 23123-23127 Q02930 denotes CREB
T9760 23123-23127 O43889 denotes CREB
T9761 23123-23127 P15336 denotes CREB
T9762 23123-23127 P16220 denotes CREB
T9763 23123-23127 P18848 denotes CREB
T9764 23203-23207 P15336 denotes CREB
T9765 23203-23207 P16220 denotes CREB
T9766 23203-23207 P18848 denotes CREB
T9767 23203-23207 O43889 denotes CREB
T9768 23203-23207 Q02930 denotes CREB
T9769 23203-23207 Q8TEY5 denotes CREB
T9770 23308-23312 P18848 denotes CREB
T9771 23308-23312 Q8TEY5 denotes CREB
T9772 23308-23312 P15336 denotes CREB
T9773 23308-23312 O43889 denotes CREB
T9774 23308-23312 Q02930 denotes CREB
T9775 23308-23312 P16220 denotes CREB
T9776 23503-23506 Q92793 denotes CBP
T9777 23772-23776 P05412 denotes AP-1
T9778 23789-23794 P05412 denotes c-jun
T9779 23791-23794 P05412 denotes jun
T9780 23850-23854 P05412 denotes AP-1
T9781 23885-23890 P05412 denotes c-jun
T9782 23887-23890 P05412 denotes jun
T9783 23964-23967 P01375 denotes TNF
T9784 23964-23969 P01375 denotes TNF-α
T9785 23992-23996 P05412 denotes AP-1
T9786 24001-24005 Q02930 denotes CREB
T9787 24001-24005 P18848 denotes CREB
T9788 24001-24005 P16220 denotes CREB
T9789 24001-24005 O43889 denotes CREB
T9790 24001-24005 P15336 denotes CREB
T9791 24001-24005 Q8TEY5 denotes CREB
T9792 24077-24080 P01375 denotes TNF
T9793 24077-24082 P01375 denotes TNF-α
T9794 24206-24210 P05412 denotes AP-1
T9795 24235-24239 O43889 denotes CREB
T9796 24235-24239 P15336 denotes CREB
T9797 24235-24239 P16220 denotes CREB
T9798 24235-24239 P18848 denotes CREB
T9799 24235-24239 Q02930 denotes CREB
T9800 24235-24239 Q8TEY5 denotes CREB
T9801 24265-24268 P01375 denotes TNF
T9802 24265-24270 P01375 denotes TNF-α
T9803 24370-24374 P16220 denotes CREB
T9804 24370-24374 O43889 denotes CREB
T9805 24370-24374 P15336 denotes CREB
T9806 24370-24374 Q8TEY5 denotes CREB
T9807 24370-24374 Q02930 denotes CREB
T9808 24370-24374 P18848 denotes CREB
T9809 24387-24391 P05412 denotes AP-1
T9810 24666-24669 P01375 denotes TNF
T9811 24666-24671 P01375 denotes TNF-α
T9812 24807-24811 P18848 denotes CREB
T9813 24807-24811 P15336 denotes CREB
T9814 24807-24811 Q02930 denotes CREB
T9815 24807-24811 Q8TEY5 denotes CREB
T9816 24807-24811 O43889 denotes CREB
T9817 24807-24811 P16220 denotes CREB
T9818 25018-25021 P01375 denotes TNF
T9819 25018-25023 P01375 denotes TNF-α
T2038 2273-2320 Q9NY61 denotes and transcription factors. Transcription factor
T2039 2277-2313 Q99853 denotes transcription factors. Transcription
T2040 2277-2313 Q9UL17 denotes transcription factors. Transcription

test2

Id Subject Object Predicate Lexical cue Negation Speculation
T29 18-28 Regulation denotes Regulation
T30 32-37 Protein denotes TNF-α
T31 38-48 Gene_expression denotes Production true
T32 294-321 Protein denotes tumor necrosis factor-alpha
T33 323-328 Protein denotes TNF-α
T34 330-340 Gene_expression denotes production
T35 423-433 Gene_expression denotes production
T36 437-442 Protein denotes TNF-α
T37 443-452 Positive_regulation denotes following
T38 583-589 Regulation denotes alters
T39 590-595 Protein denotes TNF-α
T40 596-606 Gene_expression denotes production
T41 839-853 Phosphorylation denotes phosphorylated
T42 866-871 Protein denotes I-κBα
T43 891-894 Protein denotes p65
T44 1060-1065 Protein denotes TNF-α
T45 1280-1286 Negative_regulation denotes reduce true
T46 1299-1304 Protein denotes I-κBα
T47 1305-1320 Phosphorylation denotes phosphorylation
T48 1330-1333 Protein denotes p65
T49 1334-1349 Phosphorylation denotes phosphorylation
T50 1363-1370 Binding denotes binding
T51 1416-1424 Positive_regulation denotes enhanced
T52 1524-1532 Gene_expression denotes secreted
T53 1551-1556 Protein denotes TNF-α
T1103 2558-2567 Regulation denotes regulator
T1104 2571-2594 Protein denotes tumor necrosis factor-α
T1105 2596-2601 Protein denotes TNF-α
T1106 2603-2613 Gene_expression denotes production
T1107 2616-2621 Protein denotes TNF-α
T1108 2724-2729 Protein denotes TNF-α
T1109 2730-2739 Gene_expression denotes synthesis
T1110 2747-2757 Negative_regulation denotes attenuated
T1111 2964-2978 Negative_regulation denotes downregulation
T1112 2982-2987 Protein denotes TNF-α
T1113 2988-2998 Gene_expression denotes production
T1114 4026-4033 Negative_regulation denotes inhibit
T1115 4040-4049 Regulation denotes dependent true
T1116 4107-4112 Protein denotes TNF-α
T1117 4185-4195 Negative_regulation denotes inhibition
T1118 4196-4210 Negative_regulation denotes down-regulates
T1119 4211-4216 Protein denotes TNF-α
T1120 4217-4227 Gene_expression denotes production
T1121 4875-4879 Regulation denotes role
T1122 4894-4901 Positive_regulation denotes induced
T1123 4902-4907 Protein denotes TNF-α
T1124 4908-4918 Gene_expression denotes production
T2427 5667-5681 Phosphorylation denotes phosphorylated
T2428 5682-5686 Protein denotes CREB
T2429 5688-5698 Entity denotes serine 133
T2430 5701-5715 Phosphorylation denotes phosphorylated
T2431 5716-5721 Protein denotes I-κBα
T2432 5723-5733 Entity denotes serines 32
T2433 5738-5740 Entity denotes 36
T2434 5743-5757 Phosphorylation denotes phosphorylated
T2435 5764-5767 Protein denotes p65
T2436 5784-5787 Entity denotes 276
T2437 6012-6017 Protein denotes TNF-α
T3236 8166-8173 Regulation denotes effects
T3237 8217-8224 Positive_regulation denotes induced
T3238 8225-8230 Protein denotes TNF-α
T3239 8231-8241 Gene_expression denotes production
T3240 8394-8407 Positive_regulation denotes up-regulation
T3241 8412-8427 Negative_regulation denotes down-regulation
T3242 8431-8436 Protein denotes TNF-α
T3243 8437-8447 Gene_expression denotes production
T3959 8505-8511 Regulation denotes Effect
T3960 8526-8533 Positive_regulation denotes induced
T3961 8534-8539 Protein denotes TNF-α
T3962 8540-8550 Gene_expression denotes production
T3963 8593-8598 Protein denotes TNF-α
T4121 8840-8845 Protein denotes I-κBα
T4122 8853-8856 Protein denotes p65
T4123 8862-8866 Protein denotes CREB
T4124 9829-9843 Phosphorylation denotes phosphorylated
T4125 9844-9849 Protein denotes I-κBα
T4126 9918-9932 Phosphorylation denotes phosphorylated
T4127 9939-9942 Protein denotes p65
T5611 12547-12554 Positive_regulation denotes induced
T5612 12572-12577 Protein denotes TNF-α
T5613 12578-12588 Gene_expression denotes production
T5614 12605-12609 Regulation denotes role
T5615 12620-12625 Protein denotes TNF-α
T5616 12626-12636 Gene_expression denotes production
T5617 12704-12711 Positive_regulation denotes induced
T5618 12712-12717 Protein denotes TNF-α
T5619 12718-12728 Gene_expression denotes production
T5620 12738-12747 Positive_regulation denotes following
T5621 13304-13309 Protein denotes TNF-α
T6219 13859-13866 Regulation denotes effects true
T6220 13881-13888 Positive_regulation denotes induced
T6221 13889-13894 Protein denotes TNF-α
T6222 13895-13905 Gene_expression denotes production
T6223 13939-13949 Negative_regulation denotes inhibition
T6224 13953-13958 Protein denotes TNF-α
T6225 13959-13969 Gene_expression denotes production
T6226 14090-14096 Regulation denotes effect
T6227 14100-14105 Protein denotes TNF-α
T6228 14106-14116 Gene_expression denotes production
T6452 14232-14243 Protein_catabolism denotes degradation
T6453 14247-14252 Protein denotes I-κBα
T6454 14268-14275 Entity denotes nuclear true
T6455 14276-14289 Localization denotes translocation
T6456 14322-14329 Regulation denotes effects
T6457 14340-14345 Protein denotes I-κBα
T6458 14359-14364 Protein denotes I-κBα
T6459 14365-14380 Phosphorylation denotes phosphorylation
T6460 14394-14403 Positive_regulation denotes increased
T6461 14404-14413 Positive_regulation denotes following
T6462 14522-14535 Negative_regulation denotes downregulated
T6463 14540-14547 Positive_regulation denotes induced
T6464 14548-14553 Protein denotes I-κBα
T6465 14554-14569 Phosphorylation denotes phosphorylation
T6466 14627-14630 Protein denotes p65
T6467 14631-14646 Phosphorylation denotes phosphorylation
T6468 14651-14660 Positive_regulation denotes increased
T6469 14661-14670 Positive_regulation denotes following
T6470 14734-14743 Negative_regulation denotes decreased
T6471 14748-14755 Positive_regulation denotes induced
T6472 14762-14765 Protein denotes p65
T6473 14766-14773 Entity denotes nuclear
T6474 14774-14789 Phosphorylation denotes phosphorylation
T6475 14955-14962 Entity denotes nuclear
T7077 16114-16123 Positive_regulation denotes increased
T7078 16124-16128 Protein denotes CREB
T7079 16133-16140 Binding denotes binding
T7570 16177-16183 Regulation denotes Effect true
T7571 16194-16199 Protein denotes TNF-α
T7572 16200-16210 Gene_expression denotes production
T7573 16268-16275 Positive_regulation denotes induced
T7574 16276-16290 Negative_regulation denotes downregulation
T7575 16294-16299 Protein denotes TNF-α
T7576 16300-16310 Gene_expression denotes production
T7577 16489-16497 Positive_regulation denotes resulted
T7578 16503-16512 Negative_regulation denotes reduction
T7579 16516-16521 Protein denotes TNF-α true
T7580 16720-16730 Regulation denotes modulation
T7581 16734-16739 Protein denotes TNF-α
T7582 16740-16750 Gene_expression denotes production
T7583 16849-16863 Negative_regulation denotes downregulation
T7584 16867-16872 Protein denotes TNF-α true
T7585 16873-16883 Gene_expression denotes production
T8176 17198-17203 Protein denotes TNF-α
T8177 17207-17215 Localization denotes released
T8178 17227-17236 Positive_regulation denotes following
T8179 17428-17437 Positive_regulation denotes increased
T8180 17438-17443 Protein denotes TNF-α
T8181 17444-17453 Gene_expression denotes synthesis
T8182 17673-17683 Negative_regulation denotes decreasing
T8183 17684-17689 Protein denotes TNF-α
T8184 17716-17726 Gene_expression denotes production
T8185 17778-17790 Negative_regulation denotes downregulate
T8186 17791-17796 Protein denotes TNF-α
T8187 17797-17807 Gene_expression denotes production
T8188 17887-17892 Protein denotes TNF-α
T8189 18015-18023 Negative_regulation denotes decrease
T8190 18024-18029 Protein denotes TNF-α true
T8191 18093-18104 Negative_regulation denotes attenuation
T8192 18112-18119 Positive_regulation denotes induced
T8193 18120-18125 Protein denotes TNF-α
T8194 18126-18136 Gene_expression denotes expression
T8195 18301-18310 Negative_regulation denotes decreases true
T8196 18311-18316 Protein denotes TNF-α
T8197 18317-18327 Gene_expression denotes production
T8198 18774-18785 Negative_regulation denotes attenuation
T8199 18789-18794 Protein denotes TNF-α
T8200 18795-18805 Gene_expression denotes production
T8201 19305-19312 Regulation denotes effects
T8202 19316-19321 Protein denotes TNF-α
T8203 19322-19331 Gene_expression denotes synthesis true
T8204 19409-19423 Negative_regulation denotes downregulation
T8205 19427-19435 Protein denotes TNF-αbut
T8206 20038-20041 Protein denotes p50
T8207 20042-20045 Protein denotes p65
T8208 20112-20117 Binding denotes bound
T8209 20154-20159 Protein denotes I-κBα
T8210 20322-20327 Protein denotes IKK-1
T8211 20332-20337 Protein denotes IKK-2
T8212 20353-20367 Phosphorylation denotes phosphorylates
T8213 20372-20377 Protein denotes I-κBα
T8214 20387-20402 Phosphorylation denotes Phosphorylation
T8215 20435-20446 Protein_catabolism denotes degradation
T8216 20450-20455 Protein denotes I-κBα
T8217 20473-20480 Entity denotes nuclear
T8218 20481-20494 Localization denotes translocation true
T8219 20536-20540 Binding denotes bind
T8220 20553-20560 Entity denotes regions
T8221 20716-20721 Protein denotes TNF-α
T8222 20788-20796 Regulation denotes involved true
T8223 20800-20805 Protein denotes TNF-α
T8224 20811-20821 Gene_expression denotes expression
T8225 20834-20843 Gene_expression denotes synthesis
T8226 20903-20916 Negative_regulation denotes downregulates
T8227 20929-20934 Protein denotes I-κBα
T8228 20935-20950 Phosphorylation denotes phosphorylation
T8229 20966-20969 Protein denotes p65
T8230 20970-20985 Phosphorylation denotes phosphorylation
T8231 20986-20999 Localization denotes translocation
T8232 21022-21029 Binding denotes binding true
T8233 21124-21129 Protein denotes I-κBα
T8234 21130-21145 Phosphorylation denotes phosphorylation
T8235 21223-21228 Protein denotes I-κBα
T8236 21341-21348 Regulation denotes effects
T8237 21359-21364 Protein denotes I-κBα
T8238 21493-21504 Binding denotes interaction
T8239 21513-21518 Protein denotes I-κBα
T8240 21609-21619 Regulation denotes difference
T8241 21623-21628 Protein denotes I-κBα
T8242 21629-21640 Protein_catabolism denotes degradation
T8243 21884-21891 Positive_regulation denotes induced
T8244 21892-21900 Negative_regulation denotes decrease
T8245 21904-21907 Protein denotes p65
T8246 21908-21923 Transcription denotes transcriptional
T8247 22428-22439 Negative_regulation denotes attenuation
T8248 22447-22454 Positive_regulation denotes induced
T8249 22455-22460 Protein denotes TNF-α
T8250 22461-22471 Gene_expression denotes production
T8251 22980-22987 Binding denotes binding
T8252 23471-23482 Binding denotes recruitment
T8253 23503-23506 Protein denotes CBP
T8254 23525-23531 Protein denotes p30034
T8255 23717-23724 Regulation denotes effects
T8256 23778-23783 Protein denotes c-fos
T8257 23789-23794 Protein denotes c-jun
T8258 23836-23846 Positive_regulation denotes Activation
T8259 23875-23880 Protein denotes c-fos
T8260 23885-23890 Protein denotes c-jun
T8261 23950-23960 Regulation denotes implicated
T8262 23964-23969 Protein denotes TNF-α
T8263 23970-23980 Gene_expression denotes expression
T8264 24077-24082 Protein denotes TNF-α
T8265 24254-24264 Negative_regulation denotes inhibiting
T8266 24265-24270 Protein denotes TNF-α
T8267 24276-24289 Transcription denotes transcription
T8268 24652-24665 Positive_regulation denotes downregulates
T8269 24666-24671 Protein denotes TNF-α
T8270 24672-24682 Gene_expression denotes production
T8271 25018-25023 Protein denotes TNF-α
T8272 25024-25034 Gene_expression denotes production
R18 T30 T31 themeOf TNF-α,Production
R19 T31 T29 themeOf Production,Regulation
R20 T32 T34 themeOf tumor necrosis factor-alpha,production
R21 T35 T37 themeOf production,following
R22 T36 T35 themeOf TNF-α,production
R23 T39 T40 themeOf TNF-α,production
R24 T40 T38 themeOf production,alters
R25 T42 T41 themeOf I-κBα,phosphorylated
R26 T46 T47 themeOf I-κBα,phosphorylation
R27 T47 T45 themeOf phosphorylation,reduce
R28 T48 T49 themeOf p65,phosphorylation
R29 T48 T50 themeOf p65,binding
R30 T49 T45 themeOf phosphorylation,reduce
R31 T50 T45 themeOf binding,reduce
R32 T53 T52 themeOf TNF-α,secreted
R820 T1104 T1106 themeOf tumor necrosis factor-α,production
R821 T1105 T1104 equivalentTo TNF-α,tumor necrosis factor-α
R822 T1106 T1103 themeOf production,regulator
R823 T1108 T1109 themeOf TNF-α,synthesis
R824 T1109 T1110 themeOf synthesis,attenuated
R825 T1112 T1113 themeOf TNF-α,production
R826 T1113 T1111 themeOf production,downregulation
R827 T1115 T1114 themeOf dependent,inhibit
R828 T1119 T1120 themeOf TNF-α,production
R829 T1120 T1118 themeOf production,down-regulates
R830 T1123 T1124 themeOf TNF-α,production
R831 T1124 T1122 themeOf production,induced
R1877 T2429 T2428 partOf serine 133,CREB
R1878 T2429 T2427 themeOf serine 133,phosphorylated
R1879 T2432 T2430 themeOf serines 32,phosphorylated
R1880 T2432 T2431 partOf serines 32,I-κBα
R1881 T2433 T2431 partOf 36,I-κBα
R1882 T2433 T2430 themeOf 36,phosphorylated
R2590 T3238 T3239 themeOf TNF-α,production
R2591 T3239 T3237 themeOf production,induced
R2592 T3242 T3243 themeOf TNF-α,production
R2593 T3243 T3241 themeOf production,down-regulation
R2594 T3243 T3240 themeOf production,up-regulation
R3246 T3961 T3962 themeOf TNF-α,production
R3247 T3962 T3960 themeOf production,induced
R3369 T4125 T4124 themeOf I-κBα,phosphorylated
R3370 T4127 T4126 themeOf p65,phosphorylated
R4660 T5612 T5613 themeOf TNF-α,production
R4661 T5613 T5611 themeOf production,induced
R4662 T5615 T5616 themeOf TNF-α,production
R4663 T5617 T5620 themeOf induced,following
R4664 T5618 T5619 themeOf TNF-α,production
R4665 T5619 T5620 themeOf production,following
R4666 T5619 T5617 themeOf production,induced
R5173 T6221 T6222 themeOf TNF-α,production
R5174 T6222 T6220 themeOf production,induced
R5175 T6224 T6225 themeOf TNF-α,production
R5176 T6225 T6223 themeOf production,inhibition
R5177 T6227 T6228 themeOf TNF-α,production
R5178 T6228 T6226 themeOf production,effect
R5349 T6453 T6452 themeOf I-κBα,degradation
R5350 T6454 T6455 locationOf nuclear,translocation
R5351 T6457 T6456 themeOf I-κBα,effects
R5352 T6458 T6459 themeOf I-κBα,phosphorylation
R5353 T6459 T6460 themeOf phosphorylation,increased
R5354 T6460 T6461 themeOf increased,following
R5355 T6463 T6462 themeOf induced,downregulated
R5356 T6464 T6465 themeOf I-κBα,phosphorylation
R5357 T6465 T6463 themeOf phosphorylation,induced
R5358 T6466 T6467 themeOf p65,phosphorylation
R5359 T6467 T6468 themeOf phosphorylation,increased
R5360 T6468 T6469 themeOf increased,following
R5361 T6471 T6470 themeOf induced,decreased
R5362 T6472 T6474 themeOf p65,phosphorylation
R5363 T6473 T6474 locationOf nuclear,phosphorylation
R5364 T6474 T6471 themeOf phosphorylation,induced
R5872 T7078 T7079 themeOf CREB,binding
R5873 T7079 T7077 themeOf binding,increased
R6247 T7571 T7572 themeOf TNF-α,production
R6248 T7572 T7570 themeOf production,Effect
R6249 T7574 T7573 themeOf downregulation,induced
R6250 T7575 T7576 themeOf TNF-α,production
R6251 T7576 T7574 themeOf production,downregulation
R6252 T7578 T7577 themeOf reduction,resulted
R6253 T7579 T7578 themeOf TNF-α,reduction
R6254 T7581 T7582 themeOf TNF-α,production
R6255 T7582 T7580 themeOf production,modulation
R6256 T7584 T7585 themeOf TNF-α,production
R6257 T7585 T7583 themeOf production,downregulation
R6714 T8176 T8177 themeOf TNF-α,released
R6715 T8180 T8181 themeOf TNF-α,synthesis
R6716 T8181 T8179 themeOf synthesis,increased
R6717 T8183 T8182 themeOf TNF-α,decreasing
R6718 T8183 T8184 themeOf TNF-α,production
R6719 T8186 T8187 themeOf TNF-α,production
R6720 T8187 T8185 themeOf production,downregulate
R6721 T8190 T8189 themeOf TNF-α,decrease
R6722 T8192 T8191 themeOf induced,attenuation
R6723 T8193 T8194 themeOf TNF-α,expression
R6724 T8194 T8192 themeOf expression,induced
R6725 T8196 T8197 themeOf TNF-α,production
R6726 T8197 T8195 themeOf production,decreases
R6727 T8199 T8200 themeOf TNF-α,production
R6728 T8200 T8198 themeOf production,attenuation
R6729 T8202 T8203 themeOf TNF-α,synthesis
R6730 T8203 T8201 themeOf synthesis,effects
R6731 T8205 T8204 themeOf TNF-αbut,downregulation
R6732 T8206 T8208 themeOf p50,bound
R6733 T8209 T8208 themeOf I-κBα,bound
R6734 T8213 T8212 themeOf I-κBα,phosphorylates
R6735 T8216 T8215 themeOf I-κBα,degradation
R6736 T8216 T8214 themeOf I-κBα,Phosphorylation
R6737 T8217 T8218 locationOf nuclear,translocation
R6738 T8223 T8224 themeOf TNF-α,expression
R6739 T8224 T8222 themeOf expression,involved
R6740 T8227 T8228 themeOf I-κBα,phosphorylation
R6741 T8228 T8226 themeOf phosphorylation,downregulates
R6742 T8229 T8232 themeOf p65,binding
R6743 T8229 T8231 themeOf p65,translocation
R6744 T8229 T8230 themeOf p65,phosphorylation
R6745 T8230 T8226 themeOf phosphorylation,downregulates
R6746 T8231 T8226 themeOf translocation,downregulates
R6747 T8232 T8226 themeOf binding,downregulates
R6748 T8233 T8234 themeOf I-κBα,phosphorylation
R6749 T8237 T8236 themeOf I-κBα,effects
R6750 T8239 T8238 themeOf I-κBα,interaction
R6751 T8241 T8242 themeOf I-κBα,degradation
R6752 T8242 T8240 themeOf degradation,difference
R6753 T8244 T8243 themeOf decrease,induced
R6754 T8245 T8246 themeOf p65,transcriptional
R6755 T8246 T8244 themeOf transcriptional,decrease
R6756 T8248 T8247 themeOf induced,attenuation
R6757 T8249 T8250 themeOf TNF-α,production
R6758 T8250 T8248 themeOf production,induced
R6759 T8253 T8252 themeOf CBP,recruitment
R6760 T8254 T8252 themeOf p30034,recruitment
R6761 T8256 T8255 themeOf c-fos,effects
R6762 T8257 T8255 themeOf c-jun,effects
R6763 T8258 T8261 causeOf Activation,implicated
R6764 T8259 T8258 themeOf c-fos,Activation
R6765 T8260 T8258 themeOf c-jun,Activation
R6766 T8262 T8263 themeOf TNF-α,expression
R6767 T8263 T8261 themeOf expression,implicated
R6768 T8266 T8267 themeOf TNF-α,transcription
R6769 T8267 T8265 themeOf transcription,inhibiting
R6770 T8269 T8270 themeOf TNF-α,production
R6771 T8270 T8268 themeOf production,downregulates
R6772 T8271 T8272 themeOf TNF-α,production