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PMC:2626671 / 6536-7844 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T18624 10-18 NN denotes Kinetics
T18625 19-21 IN denotes of
T18626 22-26 NN denotes gene
T18627 27-37 NN denotes expression
T18628 38-44 IN denotes during
T18629 45-49 JJ denotes CD8+
T18630 50-51 NN denotes T
T18631 52-56 NN denotes cell
T18632 57-73 NN denotes differentiation.
T18633 74-75 -LRB- denotes (
T18634 75-76 NN denotes A
T18635 76-77 -RRB- denotes )
T18636 78-86 NN denotes Kinetics
T18637 87-89 IN denotes of
T18638 90-94 NN denotes Prf1
T18639 94-95 -COMMA- denotes ,
T18640 96-100 NN denotes Gzmb
T18641 100-101 -COMMA- denotes ,
T18642 102-107 NN denotes Tbx21
T18643 108-109 -LRB- denotes (
T18644 109-114 NN denotes T-bet
T18645 114-115 -RRB- denotes )
T18646 115-116 -COMMA- denotes ,
T18647 117-120 CC denotes and
T18648 121-126 NN denotes Eomes
T18649 127-131 NN denotes mRNA
T18650 132-142 NN denotes expression
T18651 143-145 IN denotes in
T18652 146-161 VB denotes differentiating
T18653 162-165 NN denotes P14
T18654 166-170 JJ denotes CD8+
T18655 171-172 NN denotes T
T18656 173-178 NN denotes cells
T18657 179-187 VB denotes analyzed
T18658 188-190 IN denotes by
T18659 191-199 JJ denotes Northern
T18660 200-208 NN denotes blotting
T18661 210-213 NN denotes RNA
T18662 214-218 IN denotes from
T18663 219-222 NN denotes day
T18664 223-224 CD denotes 7
T18665 225-228 NN denotes Th1
T18666 229-234 NN denotes cells
T18667 235-238 VB denotes was
T18668 239-243 VB denotes used
T18669 244-246 IN denotes as
T18670 247-248 DT denotes a
T18671 249-256 NN denotes control
T18672 258-263 NN denotes Sizes
T18673 264-266 IN denotes of
T18674 267-271 NN denotes mRNA
T18675 272-283 NN denotes transcripts
T18676 284-287 VB denotes are
T18677 288-298 VB denotes indicated.
T18678 299-300 -LRB- denotes (
T18679 300-301 NN denotes B
T18680 301-302 -RRB- denotes )
T18681 303-317 NN denotes Quantification
T18682 318-320 IN denotes of
T18683 321-329 JJ denotes relative
T18684 330-334 NN denotes mRNA
T18685 335-342 NN denotes amounts
T18686 343-345 IN denotes by
T18687 346-360 NN denotes phosphorimager
T18688 361-370 NN denotes analysis.
T18689 371-372 -LRB- denotes (
T18690 372-373 NN denotes C
T18691 373-374 -RRB- denotes )
T18692 375-383 NN denotes Kinetics
T18693 384-386 IN denotes of
T18694 387-394 NN denotes protein
T18695 395-405 NN denotes expression
T18696 406-408 IN denotes in
T18697 409-424 VB denotes differentiating
T18698 425-428 NN denotes P14
T18699 429-433 JJ denotes CD8+
T18700 434-435 NN denotes T
T18701 436-441 NN denotes cells
T18702 442-450 VB denotes analyzed
T18703 451-453 IN denotes by
T18704 454-468 NN denotes immunoblotting
T18705 470-475 NN denotes Sizes
T18706 476-478 IN denotes of
T18707 479-486 NN denotes protein
T18708 487-492 NN denotes bands
T18709 493-496 VB denotes are
T18710 497-507 VB denotes indicated.
T18711 508-509 -LRB- denotes (
T18712 509-510 NN denotes D
T18713 510-511 -RRB- denotes )
T18714 512-520 JJ denotes Relative
T18715 521-528 NN denotes protein
T18716 529-536 NN denotes amounts
T18717 537-547 VB denotes quantified
T18718 548-552 IN denotes from
T18719 553-556 DT denotes the
T18720 557-564 JJ denotes Western
T18721 565-571 NN denotes blots.
T18722 572-573 -LRB- denotes (
T18723 573-574 NN denotes E
T18724 574-575 -RRB- denotes )
T18725 576-589 JJ denotes Intracellular
T18726 590-598 NN denotes staining
T18727 599-602 IN denotes for
T18728 603-611 NN denotes granzyme
T18729 612-613 NN denotes B
T18730 613-614 -COMMA- denotes ,
T18731 615-620 NN denotes IFN-γ
T18732 620-621 -COMMA- denotes ,
T18733 622-625 CC denotes and
T18734 626-629 NN denotes TNF
T18735 631-639 NN denotes Granzyme
T18736 640-641 NN denotes B
T18737 642-650 NN denotes staining
T18738 651-654 VB denotes was
T18739 655-663 JJ denotes specific
T18740 664-672 JJ denotes relative
T18741 673-675 TO denotes to
T18742 676-678 DT denotes an
T18743 679-686 NN denotes isotype
T18744 687-694 NN denotes control
T18745 695-696 -LRB- denotes (
T18746 696-699 RB denotes not
T18747 700-708 VB denotes depicted
T18748 708-709 -RRB- denotes )
T18749 711-716 NN denotes Cells
T18750 717-721 VB denotes were
T18751 722-734 VB denotes restimulated
T18752 735-739 IN denotes with
T18753 740-743 NN denotes PMA
T18754 744-747 CC denotes and
T18755 748-757 NN denotes ionomycin
T18756 758-761 IN denotes for
T18757 762-763 CD denotes 4
T18758 764-766 NN denotes h.
T18759 767-768 -LRB- denotes (
T18760 768-769 NN denotes F
T18761 769-770 -RRB- denotes )
T18762 771-781 JJ denotes FACS-based
T18763 782-787 NN denotes assay
T18764 788-790 TO denotes to
T18765 791-798 VB denotes measure
T18766 799-808 JJ denotes cytolytic
T18767 809-817 NN denotes activity
T18768 818-820 IN denotes of
T18769 821-824 NN denotes P14
T18770 825-829 JJ denotes CD8+
T18771 830-831 NN denotes T
T18772 832-837 NN denotes cells
T18773 838-845 IN denotes against
T18774 846-849 NN denotes EL4
T18775 850-857 NN denotes targets
T18776 858-864 VB denotes loaded
T18777 865-869 IN denotes with
T18778 870-871 CD denotes 0
T18779 872-873 -LRB- denotes (
T18780 873-874 JJ|RB denotes −
T18781 874-875 -RRB- denotes )
T18782 876-878 CC denotes or
T18783 879-880 CD denotes 1
T18784 881-882 -LRB- denotes (
T18785 882-883 SYM denotes +
T18786 883-884 -RRB- denotes )
T18787 885-887 NN denotes μM
T18788 888-892 NN denotes Gp33
T18789 893-900 NN denotes peptide
T18790 901-902 -LRB- denotes (
T18791 902-920 JJ denotes effector-to-target
T18792 921-926 NN denotes ratio
T18793 927-928 SYM denotes =
T18794 929-930 CD denotes 5
T18795 930-931 SYM denotes :
T18796 931-932 CD denotes 1
T18797 932-933 -RRB- denotes )
T18798 935-945 NN denotes Percentage
T18799 946-948 IN denotes of
T18800 949-956 NN denotes Annexin
T18801 957-959 JJ denotes V+
T18802 960-961 -LRB- denotes (
T18803 961-970 JJ denotes apoptotic
T18804 970-971 -RRB- denotes )
T18805 972-978 NN denotes target
T18806 979-984 NN denotes cells
T18807 985-987 IN denotes in
T18808 988-991 DT denotes the
T18809 992-1004 JJ denotes CD8-negative
T18810 1005-1008 NN denotes EL4
T18811 1009-1015 NN denotes target
T18812 1016-1026 NN denotes population
T18813 1027-1028 -LRB- denotes (
T18814 1028-1031 NN denotes dot
T18815 1032-1037 NN denotes plots
T18816 1037-1038 -RRB- denotes )
T18817 1039-1042 VB denotes was
T18818 1043-1053 VB denotes determined
T18819 1054-1055 -LRB- denotes (
T18820 1055-1065 NN denotes histograms
T18821 1065-1066 -RRB- denotes )
T18822 1068-1077 JJ denotes Cytolytic
T18823 1078-1086 NN denotes activity
T18824 1087-1090 VB denotes was
T18825 1091-1098 VB denotes blocked
T18826 1099-1101 IN denotes by
T18827 1102-1112 NN denotes incubation
T18828 1113-1117 IN denotes with
T18829 1118-1119 CD denotes 2
T18830 1120-1122 NN denotes mM
T18831 1123-1127 NN denotes EGTA
T18832 1128-1129 -LRB- denotes (
T18833 1129-1132 RB denotes not
T18834 1133-1141 VB denotes depicted
T18835 1141-1142 -RRB- denotes )
T18836 1142-1143 -COMMA- denotes ,
T18837 1144-1154 VB denotes confirming
T18838 1155-1166 NN denotes involvement
T18839 1167-1169 IN denotes of
T18840 1170-1173 DT denotes the
T18841 1174-1181 NN denotes granule
T18842 1182-1192 NN denotes exocytosis
T18843 1193-1194 -LRB- denotes (
T18844 1194-1211 NN denotes perforin–granzyme
T18845 1212-1213 NN denotes B
T18846 1213-1214 -RRB- denotes )
T18847 1215-1222 NN denotes pathway
T18848 1224-1228 NN denotes Data
T18849 1229-1232 VB denotes are
T18850 1233-1247 JJ denotes representative
T18851 1248-1250 IN denotes of
T18852 1251-1253 IN denotes at
T18853 1254-1259 JJ denotes least
T18854 1260-1264 CD denotes five
T18855 1265-1266 -LRB- denotes (
T18856 1266-1269 NN denotes A–E
T18857 1269-1270 -RRB- denotes )
T18858 1271-1273 CC denotes or
T18859 1274-1279 CD denotes three
T18860 1280-1281 -LRB- denotes (
T18861 1281-1282 NN denotes F
T18862 1282-1283 -RRB- denotes )
T18863 1284-1295 JJ denotes independent
T18864 1296-1307 NN denotes experiments
R14496 T18624 T18625 arg1Of Kinetics,of
R14497 T18624 T18628 arg1Of Kinetics,during
R14498 T18627 T18625 arg2Of expression,of
R14499 T18627 T18626 arg1Of expression,gene
R14500 T18632 T18630 arg1Of differentiation.,T
R14501 T18632 T18631 arg1Of differentiation.,cell
R14502 T18632 T18633 arg1Of differentiation.,(
R14503 T18634 T18633 arg2Of A,(
R14504 T18635 T18633 arg3Of ),(
R14505 T18636 T18628 arg2Of Kinetics,during
R14506 T18636 T18629 arg1Of Kinetics,CD8+
R14507 T18636 T18632 arg1Of Kinetics,differentiation.
R14508 T18636 T18637 arg1Of Kinetics,of
R14509 T18638 T18639 arg1Of Prf1,","
R14510 T18639 T18641 arg1Of ",",","
R14511 T18640 T18639 arg2Of Gzmb,","
R14512 T18641 T18647 arg1Of ",",and
R14513 T18642 T18641 arg2Of Tbx21,","
R14514 T18642 T18643 arg1Of Tbx21,(
R14515 T18644 T18643 arg2Of T-bet,(
R14516 T18645 T18643 arg3Of ),(
R14517 T18647 T18637 arg2Of and,of
R14518 T18647 T18646 arg1Of and,","
R14519 T18647 T18651 arg1Of and,in
R14520 T18650 T18647 arg2Of expression,and
R14521 T18650 T18648 arg1Of expression,Eomes
R14522 T18650 T18649 arg1Of expression,mRNA
R14523 T18656 T18651 arg2Of cells,in
R14524 T18656 T18652 arg1Of cells,differentiating
R14525 T18656 T18653 arg1Of cells,P14
R14526 T18656 T18654 arg1Of cells,CD8+
R14527 T18656 T18655 arg1Of cells,T
R14528 T18656 T18657 arg2Of cells,analyzed
R14529 T18660 T18657 arg1Of blotting,analyzed
R14530 T18660 T18658 arg2Of blotting,by
R14531 T18660 T18659 arg1Of blotting,Northern
R14532 T18661 T18662 arg1Of RNA,from
R14533 T18661 T18667 arg1Of RNA,was
R14534 T18661 T18668 arg2Of RNA,used
R14535 T18666 T18662 arg2Of cells,from
R14536 T18666 T18663 arg1Of cells,day
R14537 T18666 T18664 arg1Of cells,7
R14538 T18666 T18665 arg1Of cells,Th1
R14539 T18668 T18667 arg2Of used,was
R14540 T18668 T18669 arg1Of used,as
R14541 T18671 T18669 arg2Of control,as
R14542 T18671 T18670 arg1Of control,a
R14543 T18672 T18673 arg1Of Sizes,of
R14544 T18672 T18676 arg1Of Sizes,are
R14545 T18672 T18677 arg2Of Sizes,indicated.
R14546 T18675 T18673 arg2Of transcripts,of
R14547 T18675 T18674 arg1Of transcripts,mRNA
R14548 T18677 T18676 arg2Of indicated.,are
R14549 T18679 T18678 arg2Of B,(
R14550 T18680 T18678 arg3Of ),(
R14551 T18681 T18677 arg3Of Quantification,indicated.
R14552 T18681 T18678 arg1Of Quantification,(
R14553 T18681 T18682 arg1Of Quantification,of
R14554 T18681 T18686 arg1Of Quantification,by
R14555 T18685 T18682 arg2Of amounts,of
R14556 T18685 T18683 arg1Of amounts,relative
R14557 T18685 T18684 arg1Of amounts,mRNA
R14558 T18688 T18687 arg1Of analysis.,phosphorimager
R14559 T18688 T18689 arg1Of analysis.,(
R14560 T18690 T18689 arg2Of C,(
R14561 T18691 T18689 arg3Of ),(
R14562 T18692 T18686 arg2Of Kinetics,by
R14563 T18692 T18688 arg1Of Kinetics,analysis.
R14564 T18692 T18693 arg1Of Kinetics,of
R14565 T18695 T18693 arg2Of expression,of
R14566 T18695 T18694 arg1Of expression,protein
R14567 T18695 T18696 arg1Of expression,in
R14568 T18701 T18696 arg2Of cells,in
R14569 T18701 T18697 arg1Of cells,differentiating
R14570 T18701 T18698 arg1Of cells,P14
R14571 T18701 T18699 arg1Of cells,CD8+
R14572 T18701 T18700 arg1Of cells,T
R14573 T18701 T18702 arg2Of cells,analyzed
R14574 T18704 T18702 arg1Of immunoblotting,analyzed
R14575 T18704 T18703 arg2Of immunoblotting,by
R14576 T18705 T18706 arg1Of Sizes,of
R14577 T18705 T18709 arg1Of Sizes,are
R14578 T18705 T18710 arg2Of Sizes,indicated.
R14579 T18708 T18706 arg2Of bands,of
R14580 T18708 T18707 arg1Of bands,protein
R14581 T18710 T18709 arg2Of indicated.,are
R14582 T18712 T18711 arg2Of D,(
R14583 T18713 T18711 arg3Of ),(
R14584 T18716 T18710 arg3Of amounts,indicated.
R14585 T18716 T18711 arg1Of amounts,(
R14586 T18716 T18714 arg1Of amounts,Relative
R14587 T18716 T18715 arg1Of amounts,protein
R14588 T18716 T18717 arg2Of amounts,quantified
R14589 T18716 T18727 arg1Of amounts,for
R14590 T18717 T18718 arg1Of quantified,from
R14591 T18723 T18722 arg2Of E,(
R14592 T18724 T18722 arg3Of ),(
R14593 T18726 T18718 arg2Of staining,from
R14594 T18726 T18719 arg1Of staining,the
R14595 T18726 T18720 arg1Of staining,Western
R14596 T18726 T18721 arg1Of staining,blots.
R14597 T18726 T18722 arg1Of staining,(
R14598 T18726 T18725 arg1Of staining,Intracellular
R14599 T18729 T18728 arg1Of B,granzyme
R14600 T18729 T18730 arg1Of B,","
R14601 T18730 T18733 arg1Of ",",and
R14602 T18731 T18730 arg2Of IFN-γ,","
R14603 T18733 T18727 arg2Of and,for
R14604 T18733 T18732 arg1Of and,","
R14605 T18734 T18733 arg2Of TNF,and
R14606 T18737 T18735 arg1Of staining,Granzyme
R14607 T18737 T18736 arg1Of staining,B
R14608 T18737 T18738 arg1Of staining,was
R14609 T18737 T18739 arg1Of staining,specific
R14610 T18739 T18738 arg2Of specific,was
R14611 T18739 T18741 arg1Of specific,to
R14612 T18741 T18740 arg1Of to,relative
R14613 T18744 T18741 arg2Of control,to
R14614 T18744 T18742 arg1Of control,an
R14615 T18744 T18743 arg1Of control,isotype
R14616 T18744 T18745 arg1Of control,(
R14617 T18747 T18745 arg2Of depicted,(
R14618 T18747 T18746 arg1Of depicted,not
R14619 T18748 T18745 arg3Of ),(
R14620 T18749 T18750 arg1Of Cells,were
R14621 T18749 T18751 arg2Of Cells,restimulated
R14622 T18751 T18750 arg2Of restimulated,were
R14623 T18751 T18752 arg1Of restimulated,with
R14624 T18753 T18754 arg1Of PMA,and
R14625 T18754 T18752 arg2Of and,with
R14626 T18754 T18756 arg1Of and,for
R14627 T18755 T18754 arg2Of ionomycin,and
R14628 T18758 T18757 arg1Of h.,4
R14629 T18758 T18759 arg1Of h.,(
R14630 T18760 T18759 arg2Of F,(
R14631 T18761 T18759 arg3Of ),(
R14632 T18762 T18758 arg1Of FACS-based,h.
R14633 T18763 T18756 arg2Of assay,for
R14634 T18763 T18762 arg1Of assay,FACS-based
R14635 T18763 T18764 modOf assay,to
R14636 T18765 T18764 arg1Of measure,to
R14637 T18765 T18773 arg1Of measure,against
R14638 T18767 T18765 arg2Of activity,measure
R14639 T18767 T18766 arg1Of activity,cytolytic
R14640 T18767 T18768 arg1Of activity,of
R14641 T18772 T18768 arg2Of cells,of
R14642 T18772 T18769 arg1Of cells,P14
R14643 T18772 T18770 arg1Of cells,CD8+
R14644 T18772 T18771 arg1Of cells,T
R14645 T18775 T18773 arg2Of targets,against
R14646 T18775 T18774 arg1Of targets,EL4
R14647 T18775 T18776 arg2Of targets,loaded
R14648 T18776 T18777 arg1Of loaded,with
R14649 T18778 T18779 arg1Of 0,(
R14650 T18778 T18782 arg1Of 0,or
R14651 T18780 T18779 arg2Of −,(
R14652 T18781 T18779 arg3Of ),(
R14653 T18782 T18777 arg2Of or,with
R14654 T18783 T18782 arg2Of 1,or
R14655 T18783 T18784 arg1Of 1,(
R14656 T18785 T18784 arg2Of +,(
R14657 T18786 T18784 arg3Of ),(
R14658 T18789 T18765 arg3Of peptide,measure
R14659 T18789 T18787 arg1Of peptide,μM
R14660 T18789 T18788 arg1Of peptide,Gp33
R14661 T18789 T18790 arg1Of peptide,(
R14662 T18792 T18790 arg2Of ratio,(
R14663 T18792 T18791 arg1Of ratio,effector-to-target
R14664 T18792 T18795 arg1Of ratio,:
R14665 T18795 T18793 arg1Of :,=
R14666 T18795 T18794 arg1Of :,5
R14667 T18795 T18796 arg1Of :,1
R14668 T18797 T18790 arg3Of ),(
R14669 T18798 T18799 arg1Of Percentage,of
R14670 T18798 T18807 arg1Of Percentage,in
R14671 T18798 T18817 arg1Of Percentage,was
R14672 T18798 T18818 arg2Of Percentage,determined
R14673 T18806 T18799 arg2Of cells,of
R14674 T18806 T18800 arg1Of cells,Annexin
R14675 T18806 T18801 arg1Of cells,V+
R14676 T18806 T18802 arg1Of cells,(
R14677 T18806 T18803 arg1Of cells,apoptotic
R14678 T18806 T18804 arg1Of cells,)
R14679 T18806 T18805 arg1Of cells,target
R14680 T18812 T18807 arg2Of population,in
R14681 T18812 T18808 arg1Of population,the
R14682 T18812 T18809 arg1Of population,CD8-negative
R14683 T18812 T18810 arg1Of population,EL4
R14684 T18812 T18811 arg1Of population,target
R14685 T18812 T18813 arg1Of population,(
R14686 T18815 T18813 arg2Of plots,(
R14687 T18815 T18814 arg1Of plots,dot
R14688 T18816 T18813 arg3Of ),(
R14689 T18818 T18817 arg2Of determined,was
R14690 T18818 T18819 arg1Of determined,(
R14691 T18820 T18819 arg2Of histograms,(
R14692 T18821 T18819 arg3Of ),(
R14693 T18823 T18822 arg1Of activity,Cytolytic
R14694 T18823 T18824 arg1Of activity,was
R14695 T18823 T18825 arg2Of activity,blocked
R14696 T18825 T18824 arg2Of blocked,was
R14697 T18825 T18826 arg1Of blocked,by
R14698 T18825 T18836 arg1Of blocked,","
R14699 T18825 T18837 modOf blocked,confirming
R14700 T18827 T18826 arg2Of incubation,by
R14701 T18827 T18828 arg1Of incubation,with
R14702 T18829 T18830 arg1Of 2,mM
R14703 T18831 T18828 arg2Of EGTA,with
R14704 T18831 T18829 arg1Of EGTA,2
R14705 T18831 T18832 arg1Of EGTA,(
R14706 T18834 T18832 arg2Of depicted,(
R14707 T18834 T18833 arg1Of depicted,not
R14708 T18835 T18832 arg3Of ),(
R14709 T18838 T18837 arg2Of involvement,confirming
R14710 T18838 T18839 arg1Of involvement,of
R14711 T18842 T18841 arg1Of exocytosis,granule
R14712 T18842 T18843 arg1Of exocytosis,(
R14713 T18845 T18843 arg2Of B,(
R14714 T18845 T18844 arg1Of B,perforin–granzyme
R14715 T18846 T18843 arg3Of ),(
R14716 T18847 T18839 arg2Of pathway,of
R14717 T18847 T18840 arg1Of pathway,the
R14718 T18847 T18842 arg1Of pathway,exocytosis
R14719 T18848 T18849 arg1Of Data,are
R14720 T18848 T18850 arg1Of Data,representative
R14721 T18850 T18849 arg2Of representative,are
R14722 T18850 T18851 arg1Of representative,of
R14723 T18854 T18852 arg1Of five,at
R14724 T18854 T18853 arg1Of five,least
R14725 T18856 T18855 arg2Of A–E,(
R14726 T18856 T18858 arg1Of A–E,or
R14727 T18857 T18855 arg3Of ),(
R14728 T18859 T18858 arg2Of three,or
R14729 T18861 T18860 arg2Of F,(
R14730 T18862 T18860 arg3Of ),(
R14731 T18864 T18851 arg2Of experiments,of
R14732 T18864 T18854 arg1Of experiments,five
R14733 T18864 T18855 arg1Of experiments,(
R14734 T18864 T18859 arg1Of experiments,three
R14735 T18864 T18860 arg1Of experiments,(
R14736 T18864 T18863 arg1Of experiments,independent

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T18364 10-18 NNS denotes Kinetics
T18365 19-21 IN denotes of
T18366 22-26 NN denotes gene
T18367 27-37 NN denotes expression
T18368 38-44 IN denotes during
T18369 45-48 NNP denotes CD8
T18370 48-49 NNP denotes +
T18371 50-51 NNP denotes T
T18372 52-56 NN denotes cell
T18373 57-72 NN denotes differentiation
T18374 72-73 . denotes .
T18375 74-75 -LRB- denotes (
T18376 75-76 NNP denotes A
T18377 76-77 -RRB- denotes )
T18378 78-86 NNPS denotes Kinetics
T18379 87-89 IN denotes of
T18380 90-94 NNP denotes Prf1
T18381 94-95 , denotes ,
T18382 96-100 NNP denotes Gzmb
T18383 100-101 , denotes ,
T18384 102-107 NNP denotes Tbx21
T18385 108-109 -LRB- denotes (
T18386 109-114 NNP denotes T-bet
T18387 114-115 -RRB- denotes )
T18388 115-116 , denotes ,
T18389 117-120 CC denotes and
T18390 121-126 NNP denotes Eomes
T18391 127-131 NNP denotes mRNA
T18392 132-142 NN denotes expression
T18393 143-145 IN denotes in
T18394 146-161 VBG denotes differentiating
T18395 162-165 NNP denotes P14
T18396 166-169 NNP denotes CD8
T18397 169-170 VBD denotes +
T18398 171-172 NNP denotes T
T18399 173-178 NNS denotes cells
T18400 179-187 VBD denotes analyzed
T18401 188-190 IN denotes by
T18402 191-199 NNP denotes Northern
T18403 200-208 VBG denotes blotting
T18404 208-209 . denotes .
T18405 210-213 NNP denotes RNA
T18406 214-218 IN denotes from
T18407 219-222 NN denotes day
T18408 223-224 CD denotes 7
T18409 225-228 CD denotes Th1
T18410 229-234 NNS denotes cells
T18411 235-238 VBD denotes was
T18412 239-243 VBN denotes used
T18413 244-246 IN denotes as
T18414 247-248 DT denotes a
T18415 249-256 NN denotes control
T18416 256-257 . denotes .
T18417 258-263 NNS denotes Sizes
T18418 264-266 IN denotes of
T18419 267-271 NNP denotes mRNA
T18420 272-283 NNS denotes transcripts
T18421 284-287 VBP denotes are
T18422 288-297 VBN denotes indicated
T18423 297-298 . denotes .
T18424 299-300 -LRB- denotes (
T18425 300-301 NNP denotes B
T18426 301-302 -RRB- denotes )
T18427 303-317 NN denotes Quantification
T18428 318-320 IN denotes of
T18429 321-329 JJ denotes relative
T18430 330-334 NNP denotes mRNA
T18431 335-342 NNS denotes amounts
T18432 343-345 IN denotes by
T18433 346-360 NN denotes phosphorimager
T18434 361-369 NN denotes analysis
T18435 369-370 . denotes .
T18436 371-372 -LRB- denotes (
T18437 372-373 $ denotes C
T18438 373-374 -RRB- denotes )
T18439 375-383 NNPS denotes Kinetics
T18440 384-386 IN denotes of
T18441 387-394 NN denotes protein
T18442 395-405 NN denotes expression
T18443 406-408 IN denotes in
T18444 409-424 VBG denotes differentiating
T18445 425-428 NNP denotes P14
T18446 429-432 NNP denotes CD8
T18447 432-433 VBD denotes +
T18448 434-435 NNP denotes T
T18449 436-441 NNS denotes cells
T18450 442-450 VBD denotes analyzed
T18451 451-453 IN denotes by
T18452 454-468 VBG denotes immunoblotting
T18453 468-469 . denotes .
T18454 470-475 NNS denotes Sizes
T18455 476-478 IN denotes of
T18456 479-486 NN denotes protein
T18457 487-492 NNS denotes bands
T18458 493-496 VBP denotes are
T18459 497-506 VBN denotes indicated
T18460 506-507 . denotes .
T18461 508-509 -LRB- denotes (
T18462 509-510 NNP denotes D
T18463 510-511 -RRB- denotes )
T18464 512-520 JJ denotes Relative
T18465 521-528 NN denotes protein
T18466 529-536 NNS denotes amounts
T18467 537-547 VBN denotes quantified
T18468 548-552 IN denotes from
T18469 553-556 DT denotes the
T18470 557-564 JJ denotes Western
T18471 565-570 NNS denotes blots
T18472 570-571 . denotes .
T18473 572-573 -LRB- denotes (
T18474 573-574 NNP denotes E
T18475 574-575 -RRB- denotes )
T18476 576-589 NNP denotes Intracellular
T18477 590-598 VBG denotes staining
T18478 599-602 IN denotes for
T18479 603-611 NN denotes granzyme
T18480 612-613 NNP denotes B
T18481 613-614 , denotes ,
T18482 615-620 NNP denotes IFN-γ
T18483 620-621 , denotes ,
T18484 622-625 CC denotes and
T18485 626-629 NNP denotes TNF
T18486 629-630 . denotes .
T18487 631-639 NNP denotes Granzyme
T18488 640-641 NNP denotes B
T18489 642-650 VBG denotes staining
T18490 651-654 VBD denotes was
T18491 655-663 JJ denotes specific
T18492 664-672 JJ denotes relative
T18493 673-675 TO denotes to
T18494 676-678 DT denotes an
T18495 679-686 NN denotes isotype
T18496 687-694 NN denotes control
T18497 695-696 -LRB- denotes (
T18498 696-699 RB denotes not
T18499 700-708 VBN denotes depicted
T18500 708-709 -RRB- denotes )
T18501 709-710 . denotes .
T18502 711-716 NNS denotes Cells
T18503 717-721 VBD denotes were
T18504 722-734 VBN denotes restimulated
T18505 735-739 IN denotes with
T18506 740-743 NNP denotes PMA
T18507 744-747 CC denotes and
T18508 748-757 NN denotes ionomycin
T18509 758-761 IN denotes for
T18510 762-763 CD denotes 4
T18511 764-766 NN denotes h.
T18512 767-768 -LRB- denotes (
T18513 768-769 NN denotes F
T18514 769-770 -RRB- denotes )
T18515 771-781 JJ denotes FACS-based
T18516 782-787 NN denotes assay
T18517 788-790 TO denotes to
T18518 791-798 VB denotes measure
T18519 799-808 JJ denotes cytolytic
T18520 809-817 NN denotes activity
T18521 818-820 IN denotes of
T18522 821-824 NNP denotes P14
T18523 825-828 NNP denotes CD8
T18524 828-829 VBD denotes +
T18525 830-831 NNP denotes T
T18526 832-837 NNS denotes cells
T18527 838-845 IN denotes against
T18528 846-849 CD denotes EL4
T18529 850-857 NNS denotes targets
T18530 858-864 VBN denotes loaded
T18531 865-869 IN denotes with
T18532 870-871 CD denotes 0
T18533 872-873 -LRB- denotes (
T18534 873-874 NN denotes −
T18535 874-875 -RRB- denotes )
T18536 876-878 CC denotes or
T18537 879-880 CD denotes 1
T18538 881-882 -LRB- denotes (
T18539 882-883 NN denotes +
T18540 883-884 -RRB- denotes )
T18541 885-887 NNP denotes μM
T18542 888-892 NNP denotes Gp33
T18543 893-900 NN denotes peptide
T18544 901-902 -LRB- denotes (
T18545 902-920 JJ denotes effector-to-target
T18546 921-926 NN denotes ratio
T18547 927-928 SYM denotes =
T18548 929-932 CD denotes 5:1
T18549 932-933 -RRB- denotes )
T18550 933-934 . denotes .
T18551 935-945 NN denotes Percentage
T18552 946-948 IN denotes of
T18553 949-956 NNP denotes Annexin
T18554 957-958 NNP denotes V
T18555 958-959 NN denotes +
T18556 960-961 -LRB- denotes (
T18557 961-970 JJ denotes apoptotic
T18558 970-971 -RRB- denotes )
T18559 972-978 NN denotes target
T18560 979-984 NNS denotes cells
T18561 985-987 IN denotes in
T18562 988-991 DT denotes the
T18563 992-1004 JJ denotes CD8-negative
T18564 1005-1008 CD denotes EL4
T18565 1009-1015 NN denotes target
T18566 1016-1026 NN denotes population
T18567 1027-1028 -LRB- denotes (
T18568 1028-1031 VB denotes dot
T18569 1032-1037 NNS denotes plots
T18570 1037-1038 -RRB- denotes )
T18571 1039-1042 VBD denotes was
T18572 1043-1053 VBN denotes determined
T18573 1054-1055 -LRB- denotes (
T18574 1055-1065 NNS denotes histograms
T18575 1065-1066 -RRB- denotes )
T18576 1066-1067 . denotes .
T18577 1068-1077 JJ denotes Cytolytic
T18578 1078-1086 NN denotes activity
T18579 1087-1090 VBD denotes was
T18580 1091-1098 VBN denotes blocked
T18581 1099-1101 IN denotes by
T18582 1102-1112 NN denotes incubation
T18583 1113-1117 IN denotes with
T18584 1118-1119 CD denotes 2
T18585 1120-1122 NNP denotes mM
T18586 1123-1127 NNP denotes EGTA
T18587 1128-1129 -LRB- denotes (
T18588 1129-1132 RB denotes not
T18589 1133-1141 VBN denotes depicted
T18590 1141-1142 -RRB- denotes )
T18591 1142-1143 , denotes ,
T18592 1144-1154 VBG denotes confirming
T18593 1155-1166 NN denotes involvement
T18594 1167-1169 IN denotes of
T18595 1170-1173 DT denotes the
T18596 1174-1181 NN denotes granule
T18597 1182-1192 NN denotes exocytosis
T18598 1193-1194 -LRB- denotes (
T18599 1194-1202 NN denotes perforin
T18600 1203-1211 NN denotes granzyme
T18601 1212-1213 NN denotes B
T18602 1213-1214 -RRB- denotes )
T18603 1215-1222 NN denotes pathway
T18604 1222-1223 . denotes .
T18605 1224-1228 NNS denotes Data
T18606 1229-1232 VBP denotes are
T18607 1233-1247 NN denotes representative
T18608 1248-1250 IN denotes of
T18609 1251-1253 IN denotes at
T18610 1254-1259 JJS denotes least
T18611 1260-1264 CD denotes five
T18612 1265-1266 -LRB- denotes (
T18613 1266-1267 DT denotes A
T18614 1268-1269 NNP denotes E
T18615 1269-1270 -RRB- denotes )
T18616 1271-1273 CC denotes or
T18617 1274-1279 CD denotes three
T18618 1280-1281 -LRB- denotes (
T18619 1281-1282 NN denotes F
T18620 1282-1283 -RRB- denotes )
T18621 1284-1295 JJ denotes independent
T18622 1296-1307 NNS denotes experiments
T18623 1307-1308 . denotes .
R14236 T18364 T18364 ROOT Kinetics,Kinetics
R14237 T18365 T18364 prep of,Kinetics
R14238 T18366 T18367 compound gene,expression
R14239 T18367 T18365 pobj expression,of
R14240 T18368 T18364 prep during,Kinetics
R14241 T18369 T18373 compound CD8,differentiation
R14242 T18370 T18373 compound +,differentiation
R14243 T18371 T18372 compound T,cell
R14244 T18372 T18373 compound cell,differentiation
R14245 T18373 T18368 pobj differentiation,during
R14246 T18374 T18364 punct .,Kinetics
R14247 T18375 T18378 punct (,Kinetics
R14248 T18376 T18378 det A,Kinetics
R14249 T18377 T18378 punct ),Kinetics
R14250 T18378 T18392 nmod Kinetics,expression
R14251 T18379 T18378 prep of,Kinetics
R14252 T18380 T18379 pobj Prf1,of
R14253 T18381 T18380 punct ",",Prf1
R14267 T18395 T18396 compound P14,CD8
R14268 T18396 T18394 dobj CD8,differentiating
R14269 T18397 T18399 amod +,cells
R14270 T18398 T18399 compound T,cells
R14271 T18399 T18394 dobj cells,differentiating
R14272 T18400 T18399 acl analyzed,cells
R14273 T18401 T18400 agent by,analyzed
R14274 T18402 T18403 compound Northern,blotting
R14275 T18403 T18401 pobj blotting,by
R14276 T18404 T18392 punct .,expression
R14277 T18405 T18410 nmod RNA,cells
R14278 T18406 T18405 prep from,RNA
R14279 T18407 T18406 pobj day,from
R14280 T18408 T18410 nummod 7,cells
R14281 T18409 T18410 nummod Th1,cells
R14282 T18410 T18412 nsubjpass cells,used
R14283 T18411 T18412 auxpass was,used
R14284 T18412 T18412 ROOT used,used
R14285 T18413 T18412 prep as,used
R14286 T18414 T18415 det a,control
R14287 T18415 T18413 pobj control,as
R14288 T18416 T18412 punct .,used
R14289 T18417 T18422 nsubjpass Sizes,indicated
R14290 T18418 T18417 prep of,Sizes
R14291 T18419 T18420 compound mRNA,transcripts
R14292 T18420 T18418 pobj transcripts,of
R14293 T18421 T18422 auxpass are,indicated
R14294 T18422 T18422 ROOT indicated,indicated
R14295 T18423 T18422 punct .,indicated
R14296 T18424 T18427 punct (,Quantification
R14297 T18425 T18427 nmod B,Quantification
R14298 T18426 T18427 punct ),Quantification
R14299 T18427 T18427 ROOT Quantification,Quantification
R14300 T18428 T18427 prep of,Quantification
R14301 T18429 T18431 amod relative,amounts
R14302 T18430 T18431 compound mRNA,amounts
R14303 T18431 T18428 pobj amounts,of
R14304 T18432 T18427 prep by,Quantification
R14305 T18433 T18434 compound phosphorimager,analysis
R14306 T18434 T18432 pobj analysis,by
R14307 T18435 T18427 punct .,Quantification
R14308 T18436 T18437 punct (,C
R14309 T18437 T18439 nmod C,Kinetics
R14310 T18438 T18437 punct ),C
R14311 T18439 T18439 ROOT Kinetics,Kinetics
R14312 T18440 T18439 prep of,Kinetics
R14313 T18441 T18442 compound protein,expression
R14314 T18442 T18440 pobj expression,of
R14315 T18443 T18439 prep in,Kinetics
R14316 T18444 T18443 pcomp differentiating,in
R14317 T18445 T18446 compound P14,CD8
R14318 T18446 T18444 dobj CD8,differentiating
R14319 T18447 T18449 amod +,cells
R14320 T18448 T18449 compound T,cells
R14321 T18449 T18444 dobj cells,differentiating
R14338 T18466 T18466 ROOT amounts,amounts
R14339 T18467 T18466 acl quantified,amounts
R14340 T18468 T18467 prep from,quantified
R14341 T18469 T18471 det the,blots
R14342 T18470 T18471 amod Western,blots
R14343 T18471 T18468 pobj blots,from
R14344 T18472 T18466 punct .,amounts
R14345 T18473 T18476 punct (,Intracellular
R14346 T18474 T18476 nmod E,Intracellular
R14347 T18475 T18476 punct ),Intracellular
R14348 T18476 T18476 ROOT Intracellular,Intracellular
R14349 T18477 T18476 acl staining,Intracellular
R14350 T18478 T18477 prep for,staining
R14351 T18479 T18480 compound granzyme,B
R14352 T18480 T18478 pobj B,for
R14353 T18481 T18480 punct ",",B
R14354 T18482 T18480 appos IFN-γ,B
R14355 T18483 T18477 punct ",",staining
R14356 T18484 T18477 cc and,staining
R14357 T18485 T18477 conj TNF,staining
R14358 T18486 T18476 punct .,Intracellular
R14359 T18487 T18488 compound Granzyme,B
R14360 T18488 T18490 nsubj B,was
R14361 T18489 T18488 acl staining,B
R14362 T18490 T18490 ROOT was,was
R14363 T18491 T18492 amod specific,relative
R14364 T18492 T18490 acomp relative,was
R14365 T18493 T18492 prep to,relative
R14366 T18494 T18496 det an,control
R14367 T18495 T18496 compound isotype,control
R14368 T18496 T18493 pobj control,to
R14369 T18497 T18499 punct (,depicted
R14370 T18498 T18499 neg not,depicted
R14371 T18499 T18496 relcl depicted,control
R14372 T18500 T18490 punct ),was
R14373 T18501 T18490 punct .,was
R14374 T18502 T18504 nsubjpass Cells,restimulated
R14375 T18503 T18504 auxpass were,restimulated
R14376 T18504 T18504 ROOT restimulated,restimulated
R14377 T18505 T18504 prep with,restimulated
R14378 T18506 T18505 pobj PMA,with
R14379 T18507 T18506 cc and,PMA
R14380 T18508 T18506 conj ionomycin,PMA
R14381 T18509 T18504 prep for,restimulated
R14382 T18510 T18511 nummod 4,h.
R14383 T18511 T18509 pobj h.,for
R14384 T18512 T18513 punct (,F
R14385 T18513 T18511 appos F,h.
R14386 T18514 T18504 punct ),restimulated
R14387 T18515 T18516 amod FACS-based,assay
R14388 T18516 T18524 nsubj assay,+
R14389 T18517 T18518 aux to,measure
R14390 T18518 T18516 acl measure,assay
R14391 T18519 T18520 amod cytolytic,activity
R14392 T18520 T18518 dobj activity,measure
R14393 T18521 T18520 prep of,activity
R14394 T18522 T18523 compound P14,CD8
R14395 T18523 T18521 pobj CD8,of
R14396 T18524 T18524 ROOT +,+
R14397 T18525 T18526 compound T,cells
R14398 T18526 T18524 dobj cells,+
R14399 T18527 T18524 prep against,+
R14400 T18528 T18529 nummod EL4,targets
R14401 T18529 T18527 pobj targets,against
R14402 T18530 T18524 prep loaded,+
R14403 T18531 T18530 prep with,loaded
R14404 T18532 T18534 nummod 0,−
R14405 T18533 T18532 punct (,0
R14406 T18534 T18531 pobj −,with
R14407 T18535 T18530 punct ),loaded
R14408 T18536 T18524 cc or,+
R14409 T18537 T18539 nummod 1,+
R14410 T18538 T18537 punct (,1
R14411 T18539 T18543 nmod +,peptide
R14412 T18540 T18543 punct ),peptide
R14413 T18541 T18543 compound μM,peptide
R14414 T18542 T18543 compound Gp33,peptide
R14415 T18543 T18546 nmod peptide,ratio
R14416 T18544 T18546 punct (,ratio
R14417 T18545 T18546 amod effector-to-target,ratio
R14418 T18546 T18548 nsubj ratio,5:1
R14419 T18547 T18548 punct =,5:1
R14420 T18548 T18524 conj 5:1,+
R14421 T18549 T18548 punct ),5:1
R14422 T18550 T18524 punct .,+
R14423 T18551 T18572 nsubjpass Percentage,determined
R14424 T18552 T18551 prep of,Percentage
R14425 T18553 T18555 compound Annexin,+
R14426 T18554 T18555 compound V,+
R14427 T18555 T18552 pobj +,of
R14428 T18556 T18557 punct (,apoptotic
R14429 T18557 T18560 amod apoptotic,cells
R14430 T18558 T18557 punct ),apoptotic
R14431 T18559 T18560 compound target,cells
R14432 T18560 T18551 conj cells,Percentage
R14433 T18561 T18560 prep in,cells
R14434 T18562 T18566 det the,population
R14435 T18563 T18566 amod CD8-negative,population
R14436 T18564 T18566 nummod EL4,population
R14437 T18565 T18566 compound target,population
R14438 T18566 T18561 pobj population,in
R14439 T18567 T18568 punct (,dot
R14440 T18568 T18569 amod dot,plots
R14441 T18569 T18560 conj plots,cells
R14442 T18570 T18560 punct ),cells
R14443 T18571 T18572 auxpass was,determined
R14444 T18572 T18572 ROOT determined,determined
R14445 T18573 T18572 punct (,determined
R14446 T18574 T18572 oprd histograms,determined
R14447 T18575 T18572 punct ),determined
R14448 T18576 T18572 punct .,determined
R14449 T18577 T18578 amod Cytolytic,activity
R14450 T18578 T18580 nsubjpass activity,blocked
R14451 T18579 T18580 auxpass was,blocked
R14452 T18580 T18580 ROOT blocked,blocked
R14453 T18581 T18580 agent by,blocked
R14454 T18582 T18581 pobj incubation,by
R14455 T18583 T18580 prep with,blocked
R14456 T18584 T18586 nummod 2,EGTA
R14457 T18585 T18586 compound mM,EGTA
R14458 T18586 T18583 pobj EGTA,with
R14459 T18587 T18589 punct (,depicted
R14460 T18588 T18589 neg not,depicted
R14461 T18589 T18586 parataxis depicted,EGTA
R14462 T18590 T18589 punct ),depicted
R14463 T18591 T18580 punct ",",blocked
R14464 T18592 T18580 advcl confirming,blocked
R14465 T18593 T18592 dobj involvement,confirming
R14466 T18594 T18593 prep of,involvement
R14467 T18595 T18597 det the,exocytosis
R14468 T18596 T18597 compound granule,exocytosis
R14469 T18597 T18594 pobj exocytosis,of
R14470 T18598 T18601 punct (,B
R14471 T18599 T18601 nmod perforin,B
R14472 T18600 T18601 compound granzyme,B
R14473 T18601 T18597 appos B,exocytosis
R14474 T18602 T18601 punct ),B
R14475 T18603 T18597 appos pathway,exocytosis
R14476 T18604 T18580 punct .,blocked
R14477 T18605 T18606 nsubj Data,are
R14478 T18606 T18606 ROOT are,are
R14479 T18607 T18622 nmod representative,experiments
R14480 T18608 T18607 prep of,representative
R14481 T18609 T18610 advmod at,least
R14482 T18610 T18611 advmod least,five
R14483 T18611 T18608 pobj five,of
R14484 T18612 T18614 punct (,E
R14485 T18613 T18614 det A,E
R14486 T18614 T18607 appos E,representative
R14487 T18615 T18614 punct ),E
R14488 T18616 T18607 cc or,representative
R14489 T18617 T18619 nummod three,F
R14490 T18618 T18619 punct (,F
R14491 T18619 T18607 conj F,representative
R14492 T18620 T18619 punct ),F
R14493 T18621 T18622 amod independent,experiments
R14494 T18622 T18606 attr experiments,are
R14495 T18623 T18606 punct .,are
R14254 T18382 T18380 conj Gzmb,Prf1
R14255 T18383 T18382 punct ",",Gzmb
R14256 T18384 T18382 conj Tbx21,Gzmb
R14257 T18385 T18386 punct (,T-bet
R14258 T18386 T18384 appos T-bet,Tbx21
R14259 T18387 T18384 punct ),Tbx21
R14260 T18388 T18384 punct ",",Tbx21
R14261 T18389 T18384 cc and,Tbx21
R14262 T18390 T18391 compound Eomes,mRNA
R14263 T18391 T18392 compound mRNA,expression
R14264 T18392 T18392 ROOT expression,expression
R14265 T18393 T18392 prep in,expression
R14266 T18394 T18393 pcomp differentiating,in
R14322 T18450 T18449 acl analyzed,cells
R14323 T18451 T18450 agent by,analyzed
R14324 T18452 T18451 pcomp immunoblotting,by
R14325 T18453 T18439 punct .,Kinetics
R14326 T18454 T18459 nsubjpass Sizes,indicated
R14327 T18455 T18454 prep of,Sizes
R14328 T18456 T18457 compound protein,bands
R14329 T18457 T18455 pobj bands,of
R14330 T18458 T18459 auxpass are,indicated
R14331 T18459 T18459 ROOT indicated,indicated
R14332 T18460 T18459 punct .,indicated
R14333 T18461 T18462 punct (,D
R14334 T18462 T18462 ROOT D,D
R14335 T18463 T18462 punct ),D
R14336 T18464 T18466 amod Relative,amounts
R14337 T18465 T18466 compound protein,amounts

GO-BP

Id Subject Object Predicate Lexical cue
T18352 22-37 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T18353 52-72 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T18354 1182-1192 http://purl.obolibrary.org/obo/GO_0006887 denotes exocytosis

GO-CC

Id Subject Object Predicate Lexical cue
T18899 52-56 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T18900 173-178 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18901 229-234 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18902 436-441 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18903 832-837 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18904 979-984 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18905 576-589 http://purl.obolibrary.org/obo/GO_0005622 denotes Intracellular

sentences

Id Subject Object Predicate Lexical cue
T18355 10-209 Sentence denotes Kinetics of gene expression during CD8+ T cell differentiation. (A) Kinetics of Prf1, Gzmb, Tbx21 (T-bet), and Eomes mRNA expression in differentiating P14 CD8+ T cells analyzed by Northern blotting.
T18356 210-257 Sentence denotes RNA from day 7 Th1 cells was used as a control.
T18357 258-469 Sentence denotes Sizes of mRNA transcripts are indicated. (B) Quantification of relative mRNA amounts by phosphorimager analysis. (C) Kinetics of protein expression in differentiating P14 CD8+ T cells analyzed by immunoblotting.
T18358 470-630 Sentence denotes Sizes of protein bands are indicated. (D) Relative protein amounts quantified from the Western blots. (E) Intracellular staining for granzyme B, IFN-γ, and TNF.
T18359 631-710 Sentence denotes Granzyme B staining was specific relative to an isotype control (not depicted).
T18360 711-934 Sentence denotes Cells were restimulated with PMA and ionomycin for 4 h. (F) FACS-based assay to measure cytolytic activity of P14 CD8+ T cells against EL4 targets loaded with 0 (−) or 1 (+) μM Gp33 peptide (effector-to-target ratio = 5:1).
T18361 935-1067 Sentence denotes Percentage of Annexin V+ (apoptotic) target cells in the CD8-negative EL4 target population (dot plots) was determined (histograms).
T18362 1068-1223 Sentence denotes Cytolytic activity was blocked by incubation with 2 mM EGTA (not depicted), confirming involvement of the granule exocytosis (perforin–granzyme B) pathway.
T18363 1224-1308 Sentence denotes Data are representative of at least five (A–E) or three (F) independent experiments.
T40 0-9 Sentence denotes Figure 1.
T41 10-209 Sentence denotes Kinetics of gene expression during CD8+ T cell differentiation. (A) Kinetics of Prf1, Gzmb, Tbx21 (T-bet), and Eomes mRNA expression in differentiating P14 CD8+ T cells analyzed by Northern blotting.
T42 210-257 Sentence denotes RNA from day 7 Th1 cells was used as a control.
T43 258-469 Sentence denotes Sizes of mRNA transcripts are indicated. (B) Quantification of relative mRNA amounts by phosphorimager analysis. (C) Kinetics of protein expression in differentiating P14 CD8+ T cells analyzed by immunoblotting.
T44 470-630 Sentence denotes Sizes of protein bands are indicated. (D) Relative protein amounts quantified from the Western blots. (E) Intracellular staining for granzyme B, IFN-γ, and TNF.
T45 631-710 Sentence denotes Granzyme B staining was specific relative to an isotype control (not depicted).
T46 711-934 Sentence denotes Cells were restimulated with PMA and ionomycin for 4 h. (F) FACS-based assay to measure cytolytic activity of P14 CD8+ T cells against EL4 targets loaded with 0 (−) or 1 (+) μM Gp33 peptide (effector-to-target ratio = 5:1).
T47 935-1067 Sentence denotes Percentage of Annexin V+ (apoptotic) target cells in the CD8-negative EL4 target population (dot plots) was determined (histograms).
T48 1068-1223 Sentence denotes Cytolytic activity was blocked by incubation with 2 mM EGTA (not depicted), confirming involvement of the granule exocytosis (perforin–granzyme B) pathway.

events-check-again

Id Subject Object Predicate Lexical cue Speculation
T19018 45-48 Protein denotes CD8
T19019 90-94 Protein denotes Prf1
T19020 96-100 Protein denotes Gzmb
T19021 102-107 Protein denotes Tbx21
T19022 109-114 Protein denotes T-bet
T19023 121-126 Protein denotes Eomes
T19024 127-142 Transcription denotes mRNA expression true
T19025 127-142 Transcription denotes mRNA expression true
T19026 127-142 Transcription denotes mRNA expression true
T19027 127-142 Transcription denotes mRNA expression true
T19028 166-169 Protein denotes CD8
T19029 429-432 Protein denotes CD8
T19030 590-598 Gene_expression denotes staining true
T19031 590-598 Gene_expression denotes staining true
T19032 590-598 Gene_expression denotes staining true
T19033 603-613 Protein denotes granzyme B
T19034 615-620 Protein denotes IFN-γ
T19035 626-629 Protein denotes TNF
T19036 631-641 Protein denotes Granzyme B
T19037 642-650 Gene_expression denotes staining true
T19038 825-828 Protein denotes CD8
T19039 949-958 Protein denotes Annexin V
T19040 992-995 Protein denotes CD8
T19041 1194-1202 Protein denotes perforin
T19042 1203-1213 Protein denotes granzyme B
R14774 T19019 T19025 themeOf Prf1,mRNA expression
R14775 T19020 T19027 themeOf Gzmb,mRNA expression
R14776 T19021 T19026 themeOf Tbx21,mRNA expression
R14777 T19022 T19021 equivalentTo T-bet,Tbx21
R14778 T19023 T19024 themeOf Eomes,mRNA expression
R14779 T19033 T19031 themeOf granzyme B,staining
R14780 T19034 T19032 themeOf IFN-γ,staining
R14781 T19035 T19030 themeOf TNF,staining
R14782 T19036 T19037 themeOf Granzyme B,staining

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T19043 45-49 Protein denotes CD8+
T19044 90-94 Protein denotes Prf1
T19045 96-100 Protein denotes Gzmb
T19046 102-107 Protein denotes Tbx21
T19047 121-131 Protein denotes Eomes mRNA
T19048 162-165 Protein denotes P14
T19049 132-142 Gene_expression denotes expression
T19050 132-142 Gene_expression denotes expression
T19051 132-142 Gene_expression denotes expression
T19052 132-142 Gene_expression denotes expression
T19053 425-428 Protein denotes P14
T19054 603-613 Protein denotes granzyme B
T19055 615-620 Protein denotes IFN-γ
T19056 626-629 Protein denotes TNF
T19057 590-598 Gene_expression denotes staining
T19058 590-598 Gene_expression denotes staining
T19059 590-598 Gene_expression denotes staining
T19060 821-824 Protein denotes P14
T19061 885-900 Protein denotes μM Gp33 peptide
T19062 992-995 Protein denotes CD8
R14783 T19044 T19049 themeOf Prf1,expression
R14784 T19045 T19050 themeOf Gzmb,expression
R14785 T19046 T19051 themeOf Tbx21,expression
R14786 T19047 T19052 themeOf Eomes mRNA,expression
R14787 T19054 T19057 themeOf granzyme B,staining
R14788 T19055 T19058 themeOf IFN-γ,staining
R14789 T19056 T19059 themeOf TNF,staining

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Speculation
T18327 45-48 Protein denotes CD8
T18328 90-94 Protein denotes Prf1
T18329 96-100 Protein denotes Gzmb
T18330 102-107 Protein denotes Tbx21
T18331 109-114 Protein denotes T-bet
T18332 121-126 Protein denotes Eomes
T18333 127-142 Transcription denotes mRNA expression true
T18334 127-142 Transcription denotes mRNA expression true
T18335 127-142 Transcription denotes mRNA expression true
T18336 127-142 Transcription denotes mRNA expression true
T18337 166-169 Protein denotes CD8
T18338 429-432 Protein denotes CD8
T18339 590-598 Gene_expression denotes staining true
T18340 590-598 Gene_expression denotes staining true
T18341 590-598 Gene_expression denotes staining true
T18342 603-613 Protein denotes granzyme B
T18343 615-620 Protein denotes IFN-γ
T18344 626-629 Protein denotes TNF
T18345 631-641 Protein denotes Granzyme B
T18346 642-650 Gene_expression denotes staining true
T18347 825-828 Protein denotes CD8
T18348 949-958 Protein denotes Annexin V
T18349 992-995 Protein denotes CD8
T18350 1194-1202 Protein denotes perforin
T18351 1203-1213 Protein denotes granzyme B
R14227 T18328 T18334 themeOf Prf1,mRNA expression
R14228 T18329 T18336 themeOf Gzmb,mRNA expression
R14229 T18330 T18335 themeOf Tbx21,mRNA expression
R14230 T18331 T18330 equivalentTo T-bet,Tbx21
R14231 T18332 T18333 themeOf Eomes,mRNA expression
R14232 T18342 T18340 themeOf granzyme B,staining
R14233 T18343 T18341 themeOf IFN-γ,staining
R14234 T18344 T18339 themeOf TNF,staining
R14235 T18345 T18346 themeOf Granzyme B,staining

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T18865 45-48 P01732 denotes CD8
T18866 45-48 P10966 denotes CD8
T18867 90-94 P14222 denotes Prf1
T18868 96-100 P10144 denotes Gzmb
T18869 102-107 Q9UL17 denotes Tbx21
T18870 109-114 Q9UL17 denotes T-bet
T18871 121-126 O95936 denotes Eomes
T18872 166-169 P10966 denotes CD8
T18873 166-169 P01732 denotes CD8
T18874 321-329 Q04864 denotes relative
T18875 429-432 P10966 denotes CD8
T18876 429-432 P01732 denotes CD8
T18877 512-520 Q04864 denotes Relative
T18878 603-613 P10144 denotes granzyme B
T18879 615-620 P01579 denotes IFN-γ
T18880 626-629 P01375 denotes TNF
T18881 631-641 P10144 denotes Granzyme B
T18882 664-672 Q04864 denotes relative
T18883 825-828 P01732 denotes CD8
T18884 825-828 P10966 denotes CD8
T18885 949-958 P08758 denotes Annexin V
T18886 992-995 P01732 denotes CD8
T18887 992-995 P10966 denotes CD8
T18888 1194-1202 P14222 denotes perforin
T18889 1203-1213 P10144 denotes granzyme B

test2

Id Subject Object Predicate Lexical cue Speculation
T18308 45-48 Protein denotes CD8 true
T18309 90-94 Protein denotes Prf1 true
T18310 96-100 Protein denotes Gzmb true
T18311 102-107 Protein denotes Tbx21 true
T18312 109-114 Protein denotes T-bet true
T18313 121-126 Protein denotes Eomes true
T18314 127-142 Transcription denotes mRNA expression true
T18315 166-169 Protein denotes CD8 true
T18316 429-432 Protein denotes CD8
T18317 590-598 Gene_expression denotes staining
T18318 603-613 Protein denotes granzyme B
T18319 615-620 Protein denotes IFN-γ
T18320 626-629 Protein denotes TNF
T18321 631-641 Protein denotes Granzyme B
T18322 825-828 Protein denotes CD8
T18323 949-958 Protein denotes Annexin V
T18324 992-995 Protein denotes CD8
T18325 1194-1202 Protein denotes perforin
T18326 1203-1213 Protein denotes granzyme B
R14219 T18309 T18314 themeOf Prf1,mRNA expression
R14220 T18310 T18314 themeOf Gzmb,mRNA expression
R14221 T18311 T18314 themeOf Tbx21,mRNA expression
R14222 T18312 T18311 equivalentTo T-bet,Tbx21
R14223 T18313 T18314 themeOf Eomes,mRNA expression
R14224 T18318 T18317 themeOf granzyme B,staining
R14225 T18319 T18317 themeOf IFN-γ,staining
R14226 T18320 T18317 themeOf TNF,staining