PMC:2626671 / 5469-9124 JSON TXT

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2_test

Id	Subject	Object	Predicate	Lexical cue
19139168-9405358-59674043	2872-2874	9405358	denotes	18
19139168-15051762-59674044	2876-2878	15051762	denotes	19

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T3825	0-8	JJ	denotes	Distinct
T3826	9-19	NN	denotes	expression
T3827	20-28	NN	denotes	kinetics
T3828	29-31	IN	denotes	of
T3829	32-40	NN	denotes	perforin
T3830	41-44	CC	denotes	and
T3831	45-53	NN	denotes	granzyme
T3832	54-55	NN	denotes	B
T3833	56-62	IN	denotes	during
T3834	63-66	NN	denotes	CTL
T3835	67-78	NN	denotes	development
T3836	79-81	IN	denotes	in
T3837	82-89	NN	denotes	culture
T3838	90-93	PRP-DOLLAR-	denotes	Our
T3839	94-105	NN	denotes	experiments
T3840	106-114	VB	denotes	revealed
T3841	115-120	JJ	denotes	clear
T3842	121-132	NN	denotes	differences
T3843	133-135	IN	denotes	in
T3844	136-139	DT	denotes	the
T3845	140-148	NN	denotes	kinetics
T3846	149-151	IN	denotes	of
T3847	152-160	NN	denotes	perforin
T3848	160-161	-COMMA-	denotes	,
T3849	162-170	NN	denotes	granzyme
T3850	171-172	NN	denotes	B
T3851	172-173	-COMMA-	denotes	,
T3852	174-177	CC	denotes	and
T3853	178-186	NN	denotes	cytokine
T3854	187-197	NN	denotes	expression
T3855	198-204	IN	denotes	during
T3856	205-209	JJ	denotes	CD8+
T3857	210-211	NN	denotes	T
T3858	212-216	NN	denotes	cell
T3859	217-227	NN	denotes	activation
T3860	228-229	-LRB-	denotes	(
T3861	229-233	NN	denotes	Fig.
T3862	234-235	CD	denotes	1
T3863	235-236	-RRB-	denotes	)
T3864	238-243	JJ	denotes	Naive
T3865	244-245	NN	denotes	T
T3866	246-251	NN	denotes	cells
T3867	252-258	VB	denotes	showed
T3868	259-269	JJ	denotes	detectable
T3869	270-280	NN	denotes	expression
T3870	281-283	IN	denotes	of
T3871	284-292	NN	denotes	perforin
T3872	293-297	NN	denotes	mRNA
T3873	298-300	RB	denotes	as
T3874	301-305	RB	denotes	well
T3875	306-308	IN	denotes	as
T3876	309-317	NN	denotes	perforin
T3877	318-325	NN	denotes	protein
T3878	326-327	-LRB-	denotes	(
T3879	327-331	NN	denotes	Fig.
T3880	332-333	CD	denotes	1
T3881	333-334	-COMMA-	denotes	,
T3882	335-338	NN	denotes	A–D
T3883	338-339	-RRB-	denotes	)
T3884	341-349	JJ	denotes	Relative
T3885	350-352	TO	denotes	to
T3886	353-356	PRP-DOLLAR-	denotes	its
T3887	357-367	NN	denotes	expression
T3888	368-370	IN	denotes	in
T3889	371-376	JJ	denotes	naive
T3890	377-378	NN	denotes	T
T3891	379-384	NN	denotes	cells
T3892	384-385	-COMMA-	denotes	,
T3893	386-394	NN	denotes	perforin
T3894	395-396	-LRB-	denotes	(
T3895	396-400	NN	denotes	Prf1
T3896	400-401	-RRB-	denotes	)
T3897	402-406	NN	denotes	mRNA
T3898	407-417	NN	denotes	expression
T3899	418-421	VB	denotes	did
T3900	422-425	RB	denotes	not
T3901	426-434	VB	denotes	increase
T3902	435-446	RB	denotes	appreciably
T3903	447-449	IN	denotes	at
T3904	450-453	NN	denotes	day
T3905	454-455	CD	denotes	2
T3906	456-459	CC	denotes	but
T3907	460-466	VB	denotes	showed
T3908	467-468	DT	denotes	a
T3909	469-481	JJ	denotes	reproducible
T3910	482-490	NN	denotes	decrease
T3911	491-493	IN	denotes	at
T3912	494-497	NN	denotes	day
T3913	498-499	CD	denotes	4
T3914	499-500	-COMMA-	denotes	,
T3915	501-509	VB	denotes	followed
T3916	510-512	IN	denotes	by
T3917	513-519	JJ	denotes	robust
T3918	520-532	NN	denotes	reexpression
T3919	533-540	IN	denotes	between
T3920	541-545	NN	denotes	days
T3921	546-547	CD	denotes	4
T3922	548-551	CC	denotes	and
T3923	552-553	CD	denotes	8
T3924	554-555	-LRB-	denotes	(
T3925	555-559	NN	denotes	Fig.
T3926	560-561	CD	denotes	1
T3927	561-562	-COMMA-	denotes	,
T3928	563-566	NN	denotes	A–D
T3929	566-567	-RRB-	denotes	)
T3930	569-571	IN	denotes	In
T3931	572-580	NN	denotes	contrast
T3932	580-581	-COMMA-	denotes	,
T3933	582-590	NN	denotes	granzyme
T3934	591-592	NN	denotes	B
T3935	593-594	-LRB-	denotes	(
T3936	594-598	NN	denotes	Gzmb
T3937	598-599	-RRB-	denotes	)
T3938	600-604	NN	denotes	mRNA
T3939	605-608	VB	denotes	was
T3940	609-612	JJ	denotes	low
T3941	613-615	CC	denotes	or
T3942	616-628	JJ	denotes	undetectable
T3943	629-631	IN	denotes	in
T3944	632-637	JJ	denotes	naive
T3945	638-639	NN	denotes	T
T3946	640-645	NN	denotes	cells
T3947	646-649	CC	denotes	but
T3948	650-653	VB	denotes	was
T3949	654-662	RB	denotes	strongly
T3950	663-675	VB	denotes	up-regulated
T3951	676-678	IN	denotes	by
T3952	679-682	NN	denotes	day
T3953	683-684	CD	denotes	2
T3954	685-690	IN	denotes	after
T3955	691-702	NN	denotes	stimulation
T3956	703-706	CC	denotes	and
T3957	707-716	VB	denotes	increased
T3958	717-730	RB	denotes	progressively
T3959	731-736	IN	denotes	until
T3960	737-740	NN	denotes	day
T3961	741-742	CD	denotes	6
T3962	743-744	-LRB-	denotes	(
T3963	744-748	NN	denotes	Fig.
T3964	749-750	CD	denotes	1
T3965	750-751	-COMMA-	denotes	,
T3966	752-753	NN	denotes	A
T3967	754-757	CC	denotes	and
T3968	758-759	NN	denotes	B
T3969	759-760	-RRB-	denotes	)
T3970	760-761	-COLON-	denotes	;
T3971	762-771	RB	denotes	similarly
T3972	771-772	-COMMA-	denotes	,
T3973	773-781	NN	denotes	granzyme
T3974	782-783	NN	denotes	B
T3975	784-791	NN	denotes	protein
T3976	792-795	VB	denotes	was
T3977	796-805	VB	denotes	expressed
T3978	806-808	IN	denotes	by
T3979	809-812	NN	denotes	day
T3980	813-814	CD	denotes	4
T3981	815-818	CC	denotes	and
T3982	819-827	VB	denotes	remained
T3983	828-832	JJ	denotes	high
T3984	833-838	IN	denotes	until
T3985	839-842	NN	denotes	day
T3986	843-844	CD	denotes	6
T3987	845-846	-LRB-	denotes	(
T3988	846-850	NN	denotes	Fig.
T3989	851-852	CD	denotes	1
T3990	853-854	NN	denotes	E
T3991	854-855	-RRB-	denotes	)
T3992	857-859	IN	denotes	As
T3993	860-868	VB	denotes	expected
T3994	868-869	-COMMA-	denotes	,
T3995	870-871	DT	denotes	a
T3996	872-877	JJ	denotes	small
T3997	878-886	NN	denotes	fraction
T3998	887-889	IN	denotes	of
T3999	890-895	JJ	denotes	naive
T4000	896-897	NN	denotes	T
T4001	898-903	NN	denotes	cells
T4002	904-913	VB	denotes	expressed
T4003	914-917	DT	denotes	the
T4004	918-927	NN	denotes	cytokines
T4005	928-933	NN	denotes	IFN-γ
T4006	934-937	CC	denotes	and
T4007	938-941	NN	denotes	TNF
T4008	942-944	IN	denotes	in
T4009	945-953	NN	denotes	response
T4010	954-956	TO	denotes	to
T4011	957-968	NN	denotes	stimulation
T4012	968-969	-COMMA-	denotes	,
T4013	970-973	CC	denotes	and
T4014	974-978	DT	denotes	this
T4015	979-987	NN	denotes	capacity
T4016	988-997	VB	denotes	increased
T4017	998-1011	RB	denotes	significantly
T4018	1012-1014	IN	denotes	in
T4019	1015-1029	VB	denotes	differentiated
T4020	1030-1035	NN	denotes	cells
T4021	1036-1037	-LRB-	denotes	(
T4022	1037-1041	NN	denotes	Fig.
T4023	1042-1043	CD	denotes	1
T4024	1044-1045	NN	denotes	E
T4025	1045-1046	-COLON-	denotes	;
T4026	1047-1050	VB	denotes	see
T4027	1051-1055	RB	denotes	also
T4028	1056-1060	NNP	denotes	Fig.
T4029	1061-1062	CD	denotes	2
T4030	1063-1064	NN	denotes	A
T4031	1064-1065	-RRB-	denotes	)
T4032	2375-2377	PRP	denotes	We
T4033	2378-2387	VB	denotes	evaluated
T4034	2388-2405	JJ	denotes	antigen-dependent
T4035	2406-2415	JJ	denotes	cytolytic
T4036	2416-2424	NN	denotes	function
T4037	2425-2427	IN	denotes	in
T4038	2428-2429	DT	denotes	a
T4039	2430-2440	JJ	denotes	short-term
T4040	2441-2446	NN	denotes	assay
T4041	2447-2449	IN	denotes	in
T4042	2450-2455	WDT	denotes	which
T4043	2456-2462	NN	denotes	target
T4044	2463-2467	NN	denotes	cell
T4045	2468-2473	NN	denotes	death
T4046	2474-2477	VB	denotes	was
T4047	2478-2486	VB	denotes	measured
T4048	2487-2493	IN	denotes	within
T4049	2494-2495	CD	denotes	2
T4050	2496-2497	NN	denotes	h
T4051	2498-2499	-LRB-	denotes	(
T4052	2499-2503	NN	denotes	Fig.
T4053	2504-2505	CD	denotes	1
T4054	2506-2507	NN	denotes	F
T4055	2507-2508	-RRB-	denotes	)
T4056	2510-2512	IN	denotes	By
T4057	2513-2521	VB	denotes	limiting
T4058	2522-2525	DT	denotes	the
T4059	2526-2534	NN	denotes	duration
T4060	2535-2537	IN	denotes	of
T4061	2538-2541	NN	denotes	TCR
T4062	2542-2553	NN	denotes	stimulation
T4063	2553-2554	-COMMA-	denotes	,
T4064	2555-2559	DT	denotes	this
T4065	2560-2568	NN	denotes	strategy
T4066	2569-2578	VB	denotes	minimizes
T4067	2579-2588	NN	denotes	cytolysis
T4068	2589-2598	JJ	denotes	secondary
T4069	2599-2601	TO	denotes	to
T4070	2602-2605	JJ	denotes	new
T4071	2606-2610	NN	denotes	gene
T4072	2611-2621	NN	denotes	expression
T4073	2622-2628	IN	denotes	during
T4074	2629-2632	DT	denotes	the
T4075	2633-2639	NN	denotes	period
T4076	2640-2642	IN	denotes	of
T4077	2643-2646	DT	denotes	the
T4078	2647-2652	NN	denotes	assay
T4079	2654-2659	JJ	denotes	Naive
T4080	2660-2661	NN	denotes	T
T4081	2662-2667	NN	denotes	cells
T4082	2668-2671	VB	denotes	did
T4083	2672-2675	RB	denotes	not
T4084	2676-2683	VB	denotes	display
T4085	2684-2695	JJ	denotes	significant
T4086	2696-2705	JJ	denotes	cytolytic
T4087	2706-2714	NN	denotes	function
T4088	2715-2717	IN	denotes	in
T4089	2718-2722	DT	denotes	this
T4090	2723-2733	JJ	denotes	short-term
T4091	2734-2739	NN	denotes	assay
T4092	2740-2741	-LRB-	denotes	(
T4093	2741-2752	JJ	denotes	unpublished
T4094	2753-2757	NN	denotes	data
T4095	2757-2758	-RRB-	denotes	)
T4096	2758-2759	-COMMA-	denotes	,
T4097	2760-2764	RB	denotes	most
T4098	2765-2771	RB	denotes	likely
T4099	2772-2779	IN	denotes	because
T4100	2780-2784	PRP	denotes	they
T4101	2785-2792	VB	denotes	express
T4102	2793-2801	JJ	denotes	immature
T4103	2802-2803	-LRB-	denotes	(
T4104	2803-2814	JJ	denotes	unprocessed
T4105	2814-2815	-RRB-	denotes	)
T4106	2816-2821	NN	denotes	forms
T4107	2822-2824	IN	denotes	of
T4108	2825-2833	NN	denotes	perforin
T4109	2834-2837	CC	denotes	and
T4110	2838-2842	VB	denotes	lack
T4111	2843-2846	DT	denotes	the
T4112	2847-2855	NN	denotes	capacity
T4113	2856-2858	TO	denotes	to
T4114	2859-2870	VB	denotes	degranulate
T4115	2871-2872	-LRB-	denotes	(
T4116	2872-2874	CD	denotes	18
T4117	2874-2875	-COMMA-	denotes	,
T4118	2876-2878	CD	denotes	19
T4119	2878-2879	-RRB-	denotes	)
T4120	2881-2885	RB	denotes	Even
T4121	2886-2891	IN	denotes	after
T4122	2892-2902	NN	denotes	activation
T4123	2903-2906	IN	denotes	for
T4124	2907-2908	CD	denotes	2
T4125	2909-2911	CC	denotes	or
T4126	2912-2913	CD	denotes	4
T4127	2914-2915	NN	denotes	d
T4128	2915-2916	-COMMA-	denotes	,
T4129	2917-2920	DT	denotes	the
T4130	2921-2926	NN	denotes	cells
T4131	2927-2933	VB	denotes	showed
T4132	2934-2938	JJ	denotes	poor
T4133	2939-2948	JJ	denotes	cytolytic
T4134	2949-2957	NN	denotes	activity
T4135	2958-2959	-LRB-	denotes	(
T4136	2959-2963	NN	denotes	Fig.
T4137	2964-2965	CD	denotes	1
T4138	2966-2967	NN	denotes	F
T4139	2967-2968	-RRB-	denotes	)
T4140	2968-2969	-COMMA-	denotes	,
T4141	2970-2972	IN	denotes	in
T4142	2973-2981	JJ	denotes	striking
T4143	2982-2990	NN	denotes	contrast
T4144	2991-2993	TO	denotes	to
T4145	2994-2999	PRP-DOLLAR-	denotes	their
T4146	3000-3008	NN	denotes	capacity
T4147	3009-3012	IN	denotes	for
T4148	3013-3022	JJ	denotes	efficient
T4149	3023-3031	NN	denotes	cytokine
T4150	3032-3042	NN	denotes	production
T4151	3043-3044	-LRB-	denotes	(
T4152	3044-3048	NN	denotes	Fig.
T4153	3049-3050	CD	denotes	1
T4154	3051-3052	NN	denotes	E
T4155	3052-3053	-RRB-	denotes	)
T4156	3055-3059	JJ	denotes	Only
T4157	3060-3065	NN	denotes	cells
T4158	3066-3074	VB	denotes	cultured
T4159	3075-3080	IN	denotes	until
T4160	3081-3084	NN	denotes	day
T4161	3085-3086	CD	denotes	6
T4162	3087-3096	VB	denotes	displayed
T4163	3097-3103	JJ	denotes	robust
T4164	3104-3116	NN	denotes	cytotoxicity
T4165	3116-3117	-COMMA-	denotes	,
T4166	3118-3120	IN	denotes	as
T4167	3121-3127	VB	denotes	judged
T4168	3128-3130	IN	denotes	by
T4169	3131-3136	PRP-DOLLAR-	denotes	their
T4170	3137-3144	NN	denotes	ability
T4171	3145-3147	TO	denotes	to
T4172	3148-3154	VB	denotes	induce
T4173	3155-3164	NN	denotes	apoptosis
T4174	3165-3167	IN	denotes	in
T4175	3168-3169	DT	denotes	a
T4176	3170-3175	JJ	denotes	large
T4177	3176-3182	NN	denotes	number
T4178	3183-3185	IN	denotes	of
T4179	3186-3192	NN	denotes	target
T4180	3193-3198	NN	denotes	cells
T4181	3199-3200	-LRB-	denotes	(
T4182	3200-3204	NN	denotes	Fig.
T4183	3205-3206	CD	denotes	1
T4184	3207-3208	NN	denotes	F
T4185	3208-3209	-RRB-	denotes	)
T4186	3211-3216	DT	denotes	These
T4187	3217-3224	NN	denotes	results
T4188	3225-3229	VB	denotes	show
T4189	3230-3234	IN	denotes	that
T4190	3235-3240	IN	denotes	after
T4191	3241-3242	DT	denotes	a
T4192	3243-3249	JJ	denotes	strong
T4193	3250-3257	NN	denotes	priming
T4194	3258-3266	NN	denotes	stimulus
T4195	3267-3274	IN	denotes	through
T4196	3275-3279	NN	denotes	TCRs
T4197	3280-3283	CC	denotes	and
T4198	3284-3298	JJ	denotes	co-stimulatory
T4199	3299-3308	NN	denotes	receptors
T4200	3309-3311	FW	denotes	in
T4201	3312-3317	FW	denotes	vitro
T4202	3317-3318	-COMMA-	denotes	,
T4203	3319-3327	NN	denotes	granzyme
T4204	3328-3329	NN	denotes	B
T4205	3330-3340	NN	denotes	expression
T4206	3341-3344	CC	denotes	and
T4207	3345-3348	DT	denotes	the
T4208	3349-3356	NN	denotes	ability
T4209	3357-3359	TO	denotes	to
T4210	3360-3367	VB	denotes	produce
T4211	3368-3376	NN	denotes	effector
T4212	3377-3386	NN	denotes	cytokines
T4213	3387-3390	VB	denotes	are
T4214	3391-3401	VB	denotes	programmed
T4215	3402-3407	RB	denotes	early
T4216	3407-3408	-COMMA-	denotes	,
T4217	3409-3416	IN	denotes	whereas
T4218	3417-3425	NN	denotes	perforin
T4219	3426-3436	NN	denotes	expression
T4220	3437-3440	CC	denotes	and
T4221	3441-3450	JJ	denotes	cytolytic
T4222	3451-3459	NN	denotes	function
T4223	3460-3463	VB	denotes	are
T4224	3464-3471	VB	denotes	induced
T4225	3472-3477	RB	denotes	later
T4226	3477-3478	-COMMA-	denotes	,
T4227	3479-3485	IN	denotes	during
T4228	3486-3489	DT	denotes	the
T4229	3490-3495	NN	denotes	phase
T4230	3496-3498	IN	denotes	of
T4231	3499-3505	JJ	denotes	clonal
T4232	3506-3515	NN	denotes	expansion
T4233	3516-3518	IN	denotes	in
T4234	3519-3523	NN	denotes	IL-2
T4235	3525-3534	RB	denotes	Therefore
T4236	3534-3535	-COMMA-	denotes	,
T4237	3536-3539	DT	denotes	the
T4238	3540-3543	CD	denotes	two
T4239	3544-3549	JJ	denotes	major
T4240	3550-3558	NN	denotes	effector
T4241	3559-3568	NN	denotes	functions
T4242	3569-3571	IN	denotes	of
T4243	3572-3575	NN	denotes	CTL
T4244	3575-3576	-COMMA-	denotes	,
T4245	3577-3585	NN	denotes	cytokine
T4246	3586-3596	NN	denotes	production
T4247	3597-3600	CC	denotes	and
T4248	3601-3610	JJ	denotes	cytolytic
T4249	3611-3619	NN	denotes	activity
T4250	3619-3620	-COMMA-	denotes	,
T4251	3621-3624	VB	denotes	are
T4252	3625-3628	RB	denotes	not
T4253	3629-3642	RB	denotes	intrinsically
T4254	3643-3654	VB	denotes	coregulated
T18624	1077-1085	NN	denotes	Kinetics
T18625	1086-1088	IN	denotes	of
T18626	1089-1093	NN	denotes	gene
T18627	1094-1104	NN	denotes	expression
T18628	1105-1111	IN	denotes	during
T18629	1112-1116	JJ	denotes	CD8+
T18630	1117-1118	NN	denotes	T
T18631	1119-1123	NN	denotes	cell
T18632	1124-1140	NN	denotes	differentiation.
T18633	1141-1142	-LRB-	denotes	(
T18634	1142-1143	NN	denotes	A
T18635	1143-1144	-RRB-	denotes	)
T18636	1145-1153	NN	denotes	Kinetics
T18637	1154-1156	IN	denotes	of
T18638	1157-1161	NN	denotes	Prf1
T18639	1161-1162	-COMMA-	denotes	,
T18640	1163-1167	NN	denotes	Gzmb
T18641	1167-1168	-COMMA-	denotes	,
T18642	1169-1174	NN	denotes	Tbx21
T18643	1175-1176	-LRB-	denotes	(
T18644	1176-1181	NN	denotes	T-bet
T18645	1181-1182	-RRB-	denotes	)
T18646	1182-1183	-COMMA-	denotes	,
T18647	1184-1187	CC	denotes	and
T18648	1188-1193	NN	denotes	Eomes
T18649	1194-1198	NN	denotes	mRNA
T18650	1199-1209	NN	denotes	expression
T18651	1210-1212	IN	denotes	in
T18652	1213-1228	VB	denotes	differentiating
T18653	1229-1232	NN	denotes	P14
T18654	1233-1237	JJ	denotes	CD8+
T18655	1238-1239	NN	denotes	T
T18656	1240-1245	NN	denotes	cells
T18657	1246-1254	VB	denotes	analyzed
T18658	1255-1257	IN	denotes	by
T18659	1258-1266	JJ	denotes	Northern
T18660	1267-1275	NN	denotes	blotting
T18661	1277-1280	NN	denotes	RNA
T18662	1281-1285	IN	denotes	from
T18663	1286-1289	NN	denotes	day
T18664	1290-1291	CD	denotes	7
T18665	1292-1295	NN	denotes	Th1
T18666	1296-1301	NN	denotes	cells
T18667	1302-1305	VB	denotes	was
T18668	1306-1310	VB	denotes	used
T18669	1311-1313	IN	denotes	as
T18670	1314-1315	DT	denotes	a
T18671	1316-1323	NN	denotes	control
T18672	1325-1330	NN	denotes	Sizes
T18673	1331-1333	IN	denotes	of
T18674	1334-1338	NN	denotes	mRNA
T18675	1339-1350	NN	denotes	transcripts
T18676	1351-1354	VB	denotes	are
T18677	1355-1365	VB	denotes	indicated.
T18678	1366-1367	-LRB-	denotes	(
T18679	1367-1368	NN	denotes	B
T18680	1368-1369	-RRB-	denotes	)
T18681	1370-1384	NN	denotes	Quantification
T18682	1385-1387	IN	denotes	of
T18683	1388-1396	JJ	denotes	relative
T18684	1397-1401	NN	denotes	mRNA
T18685	1402-1409	NN	denotes	amounts
T18686	1410-1412	IN	denotes	by
T18687	1413-1427	NN	denotes	phosphorimager
T18688	1428-1437	NN	denotes	analysis.
T18689	1438-1439	-LRB-	denotes	(
T18690	1439-1440	NN	denotes	C
T18691	1440-1441	-RRB-	denotes	)
T18692	1442-1450	NN	denotes	Kinetics
T18693	1451-1453	IN	denotes	of
T18694	1454-1461	NN	denotes	protein
T18695	1462-1472	NN	denotes	expression
T18696	1473-1475	IN	denotes	in
T18697	1476-1491	VB	denotes	differentiating
T18698	1492-1495	NN	denotes	P14
T18699	1496-1500	JJ	denotes	CD8+
T18700	1501-1502	NN	denotes	T
T18701	1503-1508	NN	denotes	cells
T18702	1509-1517	VB	denotes	analyzed
T18703	1518-1520	IN	denotes	by
T18704	1521-1535	NN	denotes	immunoblotting
T18705	1537-1542	NN	denotes	Sizes
T18706	1543-1545	IN	denotes	of
T18707	1546-1553	NN	denotes	protein
T18708	1554-1559	NN	denotes	bands
T18709	1560-1563	VB	denotes	are
T18710	1564-1574	VB	denotes	indicated.
T18711	1575-1576	-LRB-	denotes	(
T18712	1576-1577	NN	denotes	D
T18713	1577-1578	-RRB-	denotes	)
T18714	1579-1587	JJ	denotes	Relative
T18715	1588-1595	NN	denotes	protein
T18716	1596-1603	NN	denotes	amounts
T18717	1604-1614	VB	denotes	quantified
T18718	1615-1619	IN	denotes	from
T18719	1620-1623	DT	denotes	the
T18720	1624-1631	JJ	denotes	Western
T18721	1632-1638	NN	denotes	blots.
T18722	1639-1640	-LRB-	denotes	(
T18723	1640-1641	NN	denotes	E
T18724	1641-1642	-RRB-	denotes	)
T18725	1643-1656	JJ	denotes	Intracellular
T18726	1657-1665	NN	denotes	staining
T18727	1666-1669	IN	denotes	for
T18728	1670-1678	NN	denotes	granzyme
T18729	1679-1680	NN	denotes	B
T18730	1680-1681	-COMMA-	denotes	,
T18731	1682-1687	NN	denotes	IFN-γ
T18732	1687-1688	-COMMA-	denotes	,
T18733	1689-1692	CC	denotes	and
T18734	1693-1696	NN	denotes	TNF
T18735	1698-1706	NN	denotes	Granzyme
T18736	1707-1708	NN	denotes	B
T18737	1709-1717	NN	denotes	staining
T18738	1718-1721	VB	denotes	was
T18739	1722-1730	JJ	denotes	specific
T18740	1731-1739	JJ	denotes	relative
T18741	1740-1742	TO	denotes	to
T18742	1743-1745	DT	denotes	an
T18743	1746-1753	NN	denotes	isotype
T18744	1754-1761	NN	denotes	control
T18745	1762-1763	-LRB-	denotes	(
T18746	1763-1766	RB	denotes	not
T18747	1767-1775	VB	denotes	depicted
T18748	1775-1776	-RRB-	denotes	)
T18749	1778-1783	NN	denotes	Cells
T18750	1784-1788	VB	denotes	were
T18751	1789-1801	VB	denotes	restimulated
T18752	1802-1806	IN	denotes	with
T18753	1807-1810	NN	denotes	PMA
T18754	1811-1814	CC	denotes	and
T18755	1815-1824	NN	denotes	ionomycin
T18756	1825-1828	IN	denotes	for
T18757	1829-1830	CD	denotes	4
T18758	1831-1833	NN	denotes	h.
T18759	1834-1835	-LRB-	denotes	(
T18760	1835-1836	NN	denotes	F
T18761	1836-1837	-RRB-	denotes	)
T18762	1838-1848	JJ	denotes	FACS-based
T18763	1849-1854	NN	denotes	assay
T18764	1855-1857	TO	denotes	to
T18765	1858-1865	VB	denotes	measure
T18766	1866-1875	JJ	denotes	cytolytic
T18767	1876-1884	NN	denotes	activity
T18768	1885-1887	IN	denotes	of
T18769	1888-1891	NN	denotes	P14
T18770	1892-1896	JJ	denotes	CD8+
T18771	1897-1898	NN	denotes	T
T18772	1899-1904	NN	denotes	cells
T18773	1905-1912	IN	denotes	against
T18774	1913-1916	NN	denotes	EL4
T18775	1917-1924	NN	denotes	targets
T18776	1925-1931	VB	denotes	loaded
T18777	1932-1936	IN	denotes	with
T18778	1937-1938	CD	denotes	0
T18779	1939-1940	-LRB-	denotes	(
T18780	1940-1941	JJ\|RB	denotes	−
T18781	1941-1942	-RRB-	denotes	)
T18782	1943-1945	CC	denotes	or
T18783	1946-1947	CD	denotes	1
T18784	1948-1949	-LRB-	denotes	(
T18785	1949-1950	SYM	denotes	+
T18786	1950-1951	-RRB-	denotes	)
T18787	1952-1954	NN	denotes	μM
T18788	1955-1959	NN	denotes	Gp33
T18789	1960-1967	NN	denotes	peptide
T18790	1968-1969	-LRB-	denotes	(
T18791	1969-1987	JJ	denotes	effector-to-target
T18792	1988-1993	NN	denotes	ratio
T18793	1994-1995	SYM	denotes	=
T18794	1996-1997	CD	denotes	5
T18795	1997-1998	SYM	denotes	:
T18796	1998-1999	CD	denotes	1
T18797	1999-2000	-RRB-	denotes	)
T18798	2002-2012	NN	denotes	Percentage
T18799	2013-2015	IN	denotes	of
T18800	2016-2023	NN	denotes	Annexin
T18801	2024-2026	JJ	denotes	V+
T18802	2027-2028	-LRB-	denotes	(
T18803	2028-2037	JJ	denotes	apoptotic
T18804	2037-2038	-RRB-	denotes	)
T18805	2039-2045	NN	denotes	target
T18806	2046-2051	NN	denotes	cells
T18807	2052-2054	IN	denotes	in
T18808	2055-2058	DT	denotes	the
T18809	2059-2071	JJ	denotes	CD8-negative
T18810	2072-2075	NN	denotes	EL4
T18811	2076-2082	NN	denotes	target
T18812	2083-2093	NN	denotes	population
T18813	2094-2095	-LRB-	denotes	(
T18814	2095-2098	NN	denotes	dot
T18815	2099-2104	NN	denotes	plots
T18816	2104-2105	-RRB-	denotes	)
T18817	2106-2109	VB	denotes	was
T18818	2110-2120	VB	denotes	determined
T18819	2121-2122	-LRB-	denotes	(
T18820	2122-2132	NN	denotes	histograms
T18821	2132-2133	-RRB-	denotes	)
T18822	2135-2144	JJ	denotes	Cytolytic
T18823	2145-2153	NN	denotes	activity
T18824	2154-2157	VB	denotes	was
T18825	2158-2165	VB	denotes	blocked
T18826	2166-2168	IN	denotes	by
T18827	2169-2179	NN	denotes	incubation
T18828	2180-2184	IN	denotes	with
T18829	2185-2186	CD	denotes	2
T18830	2187-2189	NN	denotes	mM
T18831	2190-2194	NN	denotes	EGTA
T18832	2195-2196	-LRB-	denotes	(
T18833	2196-2199	RB	denotes	not
T18834	2200-2208	VB	denotes	depicted
T18835	2208-2209	-RRB-	denotes	)
T18836	2209-2210	-COMMA-	denotes	,
T18837	2211-2221	VB	denotes	confirming
T18838	2222-2233	NN	denotes	involvement
T18839	2234-2236	IN	denotes	of
T18840	2237-2240	DT	denotes	the
T18841	2241-2248	NN	denotes	granule
T18842	2249-2259	NN	denotes	exocytosis
T18843	2260-2261	-LRB-	denotes	(
T18844	2261-2278	NN	denotes	perforin–granzyme
T18845	2279-2280	NN	denotes	B
T18846	2280-2281	-RRB-	denotes	)
T18847	2282-2289	NN	denotes	pathway
T18848	2291-2295	NN	denotes	Data
T18849	2296-2299	VB	denotes	are
T18850	2300-2314	JJ	denotes	representative
T18851	2315-2317	IN	denotes	of
T18852	2318-2320	IN	denotes	at
T18853	2321-2326	JJ	denotes	least
T18854	2327-2331	CD	denotes	five
T18855	2332-2333	-LRB-	denotes	(
T18856	2333-2336	NN	denotes	A–E
T18857	2336-2337	-RRB-	denotes	)
T18858	2338-2340	CC	denotes	or
T18859	2341-2346	CD	denotes	three
T18860	2347-2348	-LRB-	denotes	(
T18861	2348-2349	NN	denotes	F
T18862	2349-2350	-RRB-	denotes	)
T18863	2351-2362	JJ	denotes	independent
T18864	2363-2374	NN	denotes	experiments
R3009	T3827	T3825	arg1Of	kinetics,Distinct
R3010	T3827	T3826	arg1Of	kinetics,expression
R3011	T3827	T3828	arg1Of	kinetics,of
R3012	T3829	T3830	arg1Of	perforin,and
R3013	T3830	T3828	arg2Of	and,of
R3014	T3830	T3833	arg1Of	and,during
R3015	T3832	T3830	arg2Of	B,and
R3016	T3832	T3831	arg1Of	B,granzyme
R3017	T3835	T3833	arg2Of	development,during
R3018	T3835	T3834	arg1Of	development,CTL
R3019	T3835	T3836	arg1Of	development,in
R3020	T3837	T3836	arg2Of	culture,in
R3021	T3839	T3838	arg1Of	experiments,Our
R3022	T3839	T3840	arg1Of	experiments,revealed
R3023	T3842	T3840	arg2Of	differences,revealed
R3024	T3842	T3841	arg1Of	differences,clear
R3025	T3842	T3843	arg1Of	differences,in
R3026	T3842	T3855	arg1Of	differences,during
R3027	T3845	T3843	arg2Of	kinetics,in
R3028	T3845	T3844	arg1Of	kinetics,the
R3029	T3845	T3846	arg1Of	kinetics,of
R3030	T3847	T3848	arg1Of	perforin,","
R3031	T3848	T3852	arg1Of	",",and
R3032	T3850	T3848	arg2Of	B,","
R3033	T3850	T3849	arg1Of	B,granzyme
R3034	T3852	T3846	arg2Of	and,of
R3035	T3852	T3851	arg1Of	and,","
R3036	T3854	T3852	arg2Of	expression,and
R3037	T3854	T3853	arg1Of	expression,cytokine
R3038	T3859	T3855	arg2Of	activation,during
R3039	T3859	T3856	arg1Of	activation,CD8+
R3040	T3859	T3857	arg1Of	activation,T
R3041	T3859	T3858	arg1Of	activation,cell
R3042	T3859	T3860	arg1Of	activation,(
R3043	T3861	T3860	arg2Of	Fig.,(
R3044	T3861	T3862	arg1Of	Fig.,1
R3045	T3863	T3860	arg3Of	),(
R3046	T3866	T3864	arg1Of	cells,Naive
R3047	T3866	T3865	arg1Of	cells,T
R3048	T3866	T3867	arg1Of	cells,showed
R3049	T3869	T3868	arg1Of	expression,detectable
R3050	T3869	T3870	arg1Of	expression,of
R3051	T3869	T3875	arg1Of	expression,as
R3052	T3872	T3870	arg2Of	mRNA,of
R3053	T3872	T3871	arg1Of	mRNA,perforin
R3054	T3875	T3867	arg2Of	as,showed
R3055	T3875	T3873	arg1Of	as,as
R3056	T3875	T3874	arg1Of	as,well
R3057	T3877	T3875	arg2Of	protein,as
R3058	T3877	T3876	arg1Of	protein,perforin
R3059	T3877	T3878	arg1Of	protein,(
R3060	T3879	T3878	arg2Of	Fig.,(
R3061	T3879	T3880	arg1Of	Fig.,1
R3062	T3879	T3881	arg1Of	Fig.,","
R3063	T3882	T3881	arg2Of	A–D,","
R3064	T3883	T3878	arg3Of	),(
R3065	T3885	T3884	arg1Of	to,Relative
R3066	T3887	T3885	arg2Of	expression,to
R3067	T3887	T3886	arg1Of	expression,its
R3068	T3887	T3888	arg1Of	expression,in
R3069	T3891	T3888	arg2Of	cells,in
R3070	T3891	T3889	arg1Of	cells,naive
R3071	T3891	T3890	arg1Of	cells,T
R3072	T3895	T3894	arg2Of	Prf1,(
R3073	T3896	T3894	arg3Of	),(
R3074	T3898	T3893	arg1Of	expression,perforin
R3075	T3898	T3894	arg1Of	expression,(
R3076	T3898	T3897	arg1Of	expression,mRNA
R3077	T3898	T3899	arg1Of	expression,did
R3078	T3898	T3901	arg1Of	expression,increase
R3079	T3898	T3907	arg1Of	expression,showed
R3080	T3901	T3899	arg2Of	increase,did
R3081	T3901	T3900	arg1Of	increase,not
R3082	T3901	T3902	arg1Of	increase,appreciably
R3083	T3901	T3903	arg1Of	increase,at
R3084	T3901	T3906	arg1Of	increase,but
R3085	T3904	T3903	arg2Of	day,at
R3086	T3904	T3905	arg1Of	day,2
R3087	T3906	T3885	arg1Of	but,to
R3088	T3906	T3892	arg1Of	but,","
R3089	T3906	T3914	arg1Of	but,","
R3090	T3906	T3915	modOf	but,followed
R3091	T3907	T3906	arg2Of	showed,but
R3092	T3910	T3907	arg2Of	decrease,showed
R3093	T3910	T3908	arg1Of	decrease,a
R3094	T3910	T3909	arg1Of	decrease,reproducible
R3095	T3910	T3911	arg1Of	decrease,at
R3096	T3912	T3911	arg2Of	day,at
R3097	T3912	T3913	arg1Of	day,4
R3098	T3918	T3915	arg1Of	reexpression,followed
R3099	T3918	T3916	arg2Of	reexpression,by
R3100	T3918	T3917	arg1Of	reexpression,robust
R3101	T3918	T3919	arg1Of	reexpression,between
R3102	T3921	T3922	arg1Of	4,and
R3103	T3922	T3919	arg2Of	and,between
R3104	T3922	T3920	arg1Of	and,days
R3105	T3922	T3924	arg1Of	and,(
R3106	T3923	T3922	arg2Of	8,and
R3107	T3925	T3924	arg2Of	Fig.,(
R3108	T3925	T3926	arg1Of	Fig.,1
R3109	T3925	T3927	arg1Of	Fig.,","
R3110	T3928	T3927	arg2Of	A–D,","
R3111	T3929	T3924	arg3Of	),(
R3112	T3931	T3930	arg2Of	contrast,In
R3113	T3936	T3935	arg2Of	Gzmb,(
R3114	T3937	T3935	arg3Of	),(
R3115	T3938	T3933	arg1Of	mRNA,granzyme
R3116	T3938	T3934	arg1Of	mRNA,B
R3117	T3938	T3935	arg1Of	mRNA,(
R3118	T3938	T3939	arg1Of	mRNA,was
R3119	T3938	T3940	arg1Of	mRNA,low
R3120	T3938	T3942	arg1Of	mRNA,undetectable
R3121	T3938	T3948	arg1Of	mRNA,was
R3122	T3938	T3950	arg2Of	mRNA,up-regulated
R3123	T3938	T3957	arg2Of	mRNA,increased
R3124	T3939	T3947	arg1Of	was,but
R3125	T3940	T3941	arg1Of	low,or
R3126	T3941	T3939	arg2Of	or,was
R3127	T3941	T3943	arg1Of	or,in
R3128	T3942	T3941	arg2Of	undetectable,or
R3129	T3946	T3943	arg2Of	cells,in
R3130	T3946	T3944	arg1Of	cells,naive
R3131	T3946	T3945	arg1Of	cells,T
R3132	T3947	T3930	arg1Of	but,In
R3133	T3947	T3932	arg1Of	but,","
R3134	T3950	T3951	arg1Of	up-regulated,by
R3135	T3950	T3956	arg1Of	up-regulated,and
R3136	T3952	T3951	arg2Of	day,by
R3137	T3952	T3953	arg1Of	day,2
R3138	T3952	T3954	arg1Of	day,after
R3139	T3955	T3954	arg2Of	stimulation,after
R3140	T3956	T3947	arg2Of	and,but
R3141	T3956	T3948	arg2Of	and,was
R3142	T3956	T3949	arg1Of	and,strongly
R3143	T3957	T3956	arg2Of	increased,and
R3144	T3957	T3958	arg1Of	increased,progressively
R3145	T3957	T3959	arg1Of	increased,until
R3146	T3957	T3970	arg1Of	increased,;
R3147	T3960	T3959	arg2Of	day,until
R3148	T3960	T3961	arg1Of	day,6
R3149	T3960	T3962	arg1Of	day,(
R3150	T3963	T3962	arg2Of	Fig.,(
R3151	T3963	T3964	arg1Of	Fig.,1
R3152	T3963	T3965	arg1Of	Fig.,","
R3153	T3966	T3967	arg1Of	A,and
R3154	T3967	T3965	arg2Of	and,","
R3155	T3968	T3967	arg2Of	B,and
R3156	T3969	T3962	arg3Of	),(
R3157	T3975	T3973	arg1Of	protein,granzyme
R3158	T3975	T3974	arg1Of	protein,B
R3159	T3975	T3976	arg1Of	protein,was
R3160	T3975	T3977	arg2Of	protein,expressed
R3161	T3975	T3982	arg1Of	protein,remained
R3162	T3975	T3983	arg1Of	protein,high
R3163	T3977	T3976	arg2Of	expressed,was
R3164	T3977	T3978	arg1Of	expressed,by
R3165	T3977	T3981	arg1Of	expressed,and
R3166	T3979	T3978	arg2Of	day,by
R3167	T3979	T3980	arg1Of	day,4
R3168	T3981	T3970	arg2Of	and,;
R3169	T3981	T3971	arg1Of	and,similarly
R3170	T3981	T3972	arg1Of	and,","
R3171	T3982	T3981	arg2Of	remained,and
R3172	T3983	T3982	arg2Of	high,remained
R3173	T3983	T3984	arg1Of	high,until
R3174	T3985	T3984	arg2Of	day,until
R3175	T3985	T3986	arg1Of	day,6
R3176	T3985	T3987	arg1Of	day,(
R3177	T3990	T3987	arg2Of	E,(
R3178	T3990	T3988	arg1Of	E,Fig.
R3179	T3990	T3989	arg1Of	E,1
R3180	T3991	T3987	arg3Of	),(
R3181	T3993	T3992	arg2Of	expected,As
R3182	T3997	T3995	arg1Of	fraction,a
R3183	T3997	T3996	arg1Of	fraction,small
R3184	T3997	T3998	arg1Of	fraction,of
R3185	T3997	T4002	arg1Of	fraction,expressed
R3186	T4001	T3998	arg2Of	cells,of
R3187	T4001	T3999	arg1Of	cells,naive
R3188	T4001	T4000	arg1Of	cells,T
R3189	T4002	T4008	arg1Of	expressed,in
R3190	T4002	T4013	arg1Of	expressed,and
R3191	T4005	T4006	arg1Of	IFN-γ,and
R3192	T4006	T4002	arg2Of	and,expressed
R3193	T4006	T4003	arg1Of	and,the
R3194	T4006	T4004	arg1Of	and,cytokines
R3195	T4007	T4006	arg2Of	TNF,and
R3196	T4008	T4010	arg1Of	in,to
R3197	T4009	T4008	arg2Of	response,in
R3198	T4011	T4008	arg3Of	stimulation,in
R3199	T4013	T3992	arg1Of	and,As
R3200	T4013	T3994	arg1Of	and,","
R3201	T4013	T4012	arg1Of	and,","
R3202	T4015	T4014	arg1Of	capacity,this
R3203	T4015	T4016	arg1Of	capacity,increased
R3204	T4016	T4013	arg2Of	increased,and
R3205	T4016	T4017	arg1Of	increased,significantly
R3206	T4016	T4018	arg1Of	increased,in
R3207	T4016	T4021	arg1Of	increased,(
R3208	T4020	T4018	arg2Of	cells,in
R3209	T4020	T4019	arg2Of	cells,differentiated
R3210	T4024	T4021	arg2Of	E,(
R3211	T4024	T4022	arg1Of	E,Fig.
R3212	T4024	T4023	arg1Of	E,1
R3213	T4024	T4026	modOf	E,see
R3214	T4026	T4025	arg1Of	see,;
R3215	T4026	T4027	arg1Of	see,also
R3216	T4028	T4029	arg1Of	Fig.,2
R3217	T4030	T4026	arg2Of	A,see
R3218	T4030	T4028	arg1Of	A,Fig.
R3219	T4031	T4021	arg3Of	),(
R3220	T4032	T4033	arg1Of	We,evaluated
R3221	T4033	T4037	arg1Of	evaluated,in
R3222	T4036	T4033	arg2Of	function,evaluated
R3223	T4036	T4034	arg1Of	function,antigen-dependent
R3224	T4036	T4035	arg1Of	function,cytolytic
R3225	T4040	T4037	arg2Of	assay,in
R3226	T4040	T4038	arg1Of	assay,a
R3227	T4040	T4039	arg1Of	assay,short-term
R3228	T4040	T4041	arg2Of	assay,in
R3229	T4040	T4042	arg1Of	assay,which
R3230	T4045	T4043	arg1Of	death,target
R3231	T4045	T4044	arg1Of	death,cell
R3232	T4045	T4046	arg1Of	death,was
R3233	T4045	T4047	arg2Of	death,measured
R3234	T4047	T4041	arg1Of	measured,in
R3235	T4047	T4046	arg2Of	measured,was
R3236	T4047	T4048	arg1Of	measured,within
R3237	T4050	T4048	arg2Of	h,within
R3238	T4050	T4049	arg1Of	h,2
R3239	T4050	T4051	arg1Of	h,(
R3240	T4054	T4051	arg2Of	F,(
R3241	T4054	T4052	arg1Of	F,Fig.
R3242	T4054	T4053	arg1Of	F,1
R3243	T4055	T4051	arg3Of	),(
R3244	T4057	T4056	arg2Of	limiting,By
R3245	T4059	T4057	arg2Of	duration,limiting
R3246	T4059	T4058	arg1Of	duration,the
R3247	T4059	T4060	arg1Of	duration,of
R3248	T4062	T4060	arg2Of	stimulation,of
R3249	T4062	T4061	arg1Of	stimulation,TCR
R3250	T4065	T4057	arg1Of	strategy,limiting
R3251	T4065	T4064	arg1Of	strategy,this
R3252	T4065	T4066	arg1Of	strategy,minimizes
R3253	T4066	T4056	arg1Of	minimizes,By
R3254	T4066	T4063	arg1Of	minimizes,","
R3255	T4066	T4073	arg1Of	minimizes,during
R3256	T4067	T4066	arg2Of	cytolysis,minimizes
R3257	T4067	T4068	arg1Of	cytolysis,secondary
R3258	T4068	T4069	arg1Of	secondary,to
R3259	T4072	T4069	arg2Of	expression,to
R3260	T4072	T4070	arg1Of	expression,new
R3261	T4072	T4071	arg1Of	expression,gene
R3262	T4075	T4073	arg2Of	period,during
R3263	T4075	T4074	arg1Of	period,the
R3264	T4075	T4076	arg1Of	period,of
R3265	T4078	T4076	arg2Of	assay,of
R3266	T4078	T4077	arg1Of	assay,the
R3267	T4081	T4079	arg1Of	cells,Naive
R3268	T4081	T4080	arg1Of	cells,T
R3269	T4081	T4082	arg1Of	cells,did
R3270	T4081	T4084	arg1Of	cells,display
R3271	T4084	T4082	arg2Of	display,did
R3272	T4084	T4083	arg1Of	display,not
R3273	T4084	T4088	arg1Of	display,in
R3274	T4084	T4096	arg1Of	display,","
R3275	T4084	T4099	arg1Of	display,because
R3276	T4086	T4085	arg1Of	cytolytic,significant
R3277	T4087	T4084	arg2Of	function,display
R3278	T4087	T4086	arg1Of	function,cytolytic
R3279	T4091	T4088	arg2Of	assay,in
R3280	T4091	T4089	arg1Of	assay,this
R3281	T4091	T4090	arg1Of	assay,short-term
R3282	T4091	T4092	arg1Of	assay,(
R3283	T4094	T4092	arg2Of	data,(
R3284	T4094	T4093	arg1Of	data,unpublished
R3285	T4095	T4092	arg3Of	),(
R3286	T4098	T4097	arg1Of	likely,most
R3287	T4099	T4098	arg1Of	because,likely
R3288	T4100	T4101	arg1Of	they,express
R3289	T4100	T4110	arg1Of	they,lack
R3290	T4101	T4109	arg1Of	express,and
R3291	T4102	T4103	arg1Of	immature,(
R3292	T4104	T4103	arg2Of	unprocessed,(
R3293	T4105	T4103	arg3Of	),(
R3294	T4106	T4101	arg2Of	forms,express
R3295	T4106	T4102	arg1Of	forms,immature
R3296	T4106	T4107	arg1Of	forms,of
R3297	T4108	T4107	arg2Of	perforin,of
R3298	T4109	T4099	arg2Of	and,because
R3299	T4110	T4109	arg2Of	lack,and
R3300	T4110	T4115	arg1Of	lack,(
R3301	T4112	T4110	arg2Of	capacity,lack
R3302	T4112	T4111	arg1Of	capacity,the
R3303	T4112	T4113	modOf	capacity,to
R3304	T4114	T4113	arg1Of	degranulate,to
R3305	T4116	T4115	arg2Of	18,(
R3306	T4116	T4117	arg1Of	18,","
R3307	T4118	T4117	arg2Of	19,","
R3308	T4119	T4115	arg3Of	),(
R3309	T4121	T4120	arg1Of	after,Even
R3310	T4122	T4121	arg2Of	activation,after
R3311	T4122	T4123	arg1Of	activation,for
R3312	T4124	T4125	arg1Of	2,or
R3313	T4126	T4125	arg2Of	4,or
R3314	T4127	T4123	arg2Of	d,for
R3315	T4127	T4124	arg1Of	d,2
R3316	T4127	T4126	arg1Of	d,4
R3317	T4130	T4129	arg1Of	cells,the
R3318	T4130	T4131	arg1Of	cells,showed
R3319	T4131	T4121	arg1Of	showed,after
R3320	T4131	T4128	arg1Of	showed,","
R3321	T4133	T4132	arg1Of	cytolytic,poor
R3322	T4134	T4131	arg2Of	activity,showed
R3323	T4134	T4133	arg1Of	activity,cytolytic
R3324	T4134	T4135	arg1Of	activity,(
R3325	T4134	T4140	arg1Of	activity,","
R3326	T4134	T4141	arg1Of	activity,in
R3327	T4138	T4135	arg2Of	F,(
R3328	T4138	T4136	arg1Of	F,Fig.
R3329	T4138	T4137	arg1Of	F,1
R3330	T4139	T4135	arg3Of	),(
R3331	T4143	T4141	arg2Of	contrast,in
R3332	T4143	T4142	arg1Of	contrast,striking
R3333	T4143	T4144	arg1Of	contrast,to
R3334	T4146	T4144	arg2Of	capacity,to
R3335	T4146	T4145	arg1Of	capacity,their
R3336	T4146	T4147	arg1Of	capacity,for
R3337	T4150	T4147	arg2Of	production,for
R3338	T4150	T4148	arg1Of	production,efficient
R3339	T4150	T4149	arg1Of	production,cytokine
R3340	T4150	T4151	arg1Of	production,(
R3341	T4154	T4151	arg2Of	E,(
R3342	T4154	T4152	arg1Of	E,Fig.
R3343	T4154	T4153	arg1Of	E,1
R3344	T4155	T4151	arg3Of	),(
R3345	T4157	T4156	arg1Of	cells,Only
R3346	T4157	T4158	arg2Of	cells,cultured
R3347	T4157	T4162	arg1Of	cells,displayed
R3348	T4158	T4159	arg1Of	cultured,until
R3349	T4160	T4159	arg2Of	day,until
R3350	T4160	T4161	arg1Of	day,6
R3351	T4162	T4165	arg1Of	displayed,","
R3352	T4162	T4166	arg1Of	displayed,as
R3353	T4164	T4162	arg2Of	cytotoxicity,displayed
R3354	T4164	T4163	arg1Of	cytotoxicity,robust
R3355	T4167	T4166	arg2Of	judged,as
R3356	T4170	T4167	arg1Of	ability,judged
R3357	T4170	T4168	arg2Of	ability,by
R3358	T4170	T4169	arg1Of	ability,their
R3359	T4170	T4171	modOf	ability,to
R3360	T4170	T4181	arg1Of	ability,(
R3361	T4172	T4171	arg1Of	induce,to
R3362	T4172	T4174	arg1Of	induce,in
R3363	T4173	T4172	arg2Of	apoptosis,induce
R3364	T4177	T4174	arg2Of	number,in
R3365	T4177	T4175	arg1Of	number,a
R3366	T4177	T4176	arg1Of	number,large
R3367	T4177	T4178	arg1Of	number,of
R3368	T4180	T4178	arg2Of	cells,of
R3369	T4180	T4179	arg1Of	cells,target
R3370	T4184	T4181	arg2Of	F,(
R3371	T4184	T4182	arg1Of	F,Fig.
R3372	T4184	T4183	arg1Of	F,1
R3373	T4185	T4181	arg3Of	),(
R3374	T4187	T4186	arg1Of	results,These
R3375	T4187	T4188	arg1Of	results,show
R3376	T4194	T4190	arg2Of	stimulus,after
R3377	T4194	T4191	arg1Of	stimulus,a
R3378	T4194	T4192	arg1Of	stimulus,strong
R3379	T4194	T4193	arg1Of	stimulus,priming
R3380	T4194	T4195	arg1Of	stimulus,through
R3381	T4196	T4197	arg1Of	TCRs,and
R3382	T4197	T4195	arg2Of	and,through
R3383	T4197	T4201	arg1Of	and,vitro
R3384	T4199	T4197	arg2Of	receptors,and
R3385	T4199	T4198	arg1Of	receptors,co-stimulatory
R3386	T4201	T4200	arg1Of	vitro,in
R3387	T4205	T4203	arg1Of	expression,granzyme
R3388	T4205	T4204	arg1Of	expression,B
R3389	T4205	T4206	arg1Of	expression,and
R3390	T4206	T4213	arg1Of	and,are
R3391	T4206	T4214	arg2Of	and,programmed
R3392	T4208	T4206	arg2Of	ability,and
R3393	T4208	T4207	arg1Of	ability,the
R3394	T4208	T4209	modOf	ability,to
R3395	T4210	T4209	arg1Of	produce,to
R3396	T4212	T4210	arg2Of	cytokines,produce
R3397	T4212	T4211	arg1Of	cytokines,effector
R3398	T4214	T4188	arg2Of	programmed,show
R3399	T4214	T4189	arg1Of	programmed,that
R3400	T4214	T4190	arg1Of	programmed,after
R3401	T4214	T4202	arg1Of	programmed,","
R3402	T4214	T4213	arg2Of	programmed,are
R3403	T4214	T4215	arg1Of	programmed,early
R3404	T4214	T4216	arg1Of	programmed,","
R3405	T4214	T4217	arg1Of	programmed,whereas
R3406	T4214	T4226	arg1Of	programmed,","
R3407	T4214	T4227	arg1Of	programmed,during
R3408	T4219	T4218	arg1Of	expression,perforin
R3409	T4219	T4220	arg1Of	expression,and
R3410	T4220	T4223	arg1Of	and,are
R3411	T4220	T4224	arg2Of	and,induced
R3412	T4222	T4220	arg2Of	function,and
R3413	T4222	T4221	arg1Of	function,cytolytic
R3414	T4224	T4217	arg2Of	induced,whereas
R3415	T4224	T4223	arg2Of	induced,are
R3416	T4224	T4225	arg1Of	induced,later
R3417	T4229	T4227	arg2Of	phase,during
R3418	T4229	T4228	arg1Of	phase,the
R3419	T4229	T4230	arg1Of	phase,of
R3420	T4232	T4230	arg2Of	expansion,of
R3421	T4232	T4231	arg1Of	expansion,clonal
R3422	T4232	T4233	arg1Of	expansion,in
R3423	T4234	T4233	arg2Of	IL-2,in
R3424	T4241	T4237	arg1Of	functions,the
R3425	T4241	T4238	arg1Of	functions,two
R3426	T4241	T4239	arg1Of	functions,major
R3427	T4241	T4240	arg1Of	functions,effector
R3428	T4241	T4242	arg1Of	functions,of
R3429	T4241	T4251	arg1Of	functions,are
R3430	T4241	T4254	arg2Of	functions,coregulated
R3431	T4243	T4244	arg1Of	CTL,","
R3432	T4244	T4242	arg2Of	",",of
R3433	T4246	T4245	arg1Of	production,cytokine
R3434	T4246	T4247	arg1Of	production,and
R3435	T4247	T4244	arg2Of	and,","
R3436	T4249	T4247	arg2Of	activity,and
R3437	T4249	T4248	arg1Of	activity,cytolytic
R3438	T4254	T4235	arg1Of	coregulated,Therefore
R3439	T4254	T4236	arg1Of	coregulated,","
R3440	T4254	T4250	arg1Of	coregulated,","
R3441	T4254	T4251	arg2Of	coregulated,are
R3442	T4254	T4252	arg1Of	coregulated,not
R3443	T4254	T4253	arg1Of	coregulated,intrinsically
R14496	T18624	T18625	arg1Of	Kinetics,of
R14497	T18624	T18628	arg1Of	Kinetics,during
R14498	T18627	T18625	arg2Of	expression,of
R14499	T18627	T18626	arg1Of	expression,gene
R14500	T18632	T18630	arg1Of	differentiation.,T
R14501	T18632	T18631	arg1Of	differentiation.,cell
R14502	T18632	T18633	arg1Of	differentiation.,(
R14503	T18634	T18633	arg2Of	A,(
R14504	T18635	T18633	arg3Of	),(
R14505	T18636	T18628	arg2Of	Kinetics,during
R14506	T18636	T18629	arg1Of	Kinetics,CD8+
R14507	T18636	T18632	arg1Of	Kinetics,differentiation.
R14508	T18636	T18637	arg1Of	Kinetics,of
R14509	T18638	T18639	arg1Of	Prf1,","
R14510	T18639	T18641	arg1Of	",",","
R14511	T18640	T18639	arg2Of	Gzmb,","
R14512	T18641	T18647	arg1Of	",",and
R14513	T18642	T18641	arg2Of	Tbx21,","
R14514	T18642	T18643	arg1Of	Tbx21,(
R14515	T18644	T18643	arg2Of	T-bet,(
R14516	T18645	T18643	arg3Of	),(
R14517	T18647	T18637	arg2Of	and,of
R14518	T18647	T18646	arg1Of	and,","
R14519	T18647	T18651	arg1Of	and,in
R14520	T18650	T18647	arg2Of	expression,and
R14521	T18650	T18648	arg1Of	expression,Eomes
R14522	T18650	T18649	arg1Of	expression,mRNA
R14523	T18656	T18651	arg2Of	cells,in
R14524	T18656	T18652	arg1Of	cells,differentiating
R14525	T18656	T18653	arg1Of	cells,P14
R14526	T18656	T18654	arg1Of	cells,CD8+
R14527	T18656	T18655	arg1Of	cells,T
R14528	T18656	T18657	arg2Of	cells,analyzed
R14529	T18660	T18657	arg1Of	blotting,analyzed
R14530	T18660	T18658	arg2Of	blotting,by
R14531	T18660	T18659	arg1Of	blotting,Northern
R14532	T18661	T18662	arg1Of	RNA,from
R14533	T18661	T18667	arg1Of	RNA,was
R14534	T18661	T18668	arg2Of	RNA,used
R14535	T18666	T18662	arg2Of	cells,from
R14536	T18666	T18663	arg1Of	cells,day
R14537	T18666	T18664	arg1Of	cells,7
R14538	T18666	T18665	arg1Of	cells,Th1
R14539	T18668	T18667	arg2Of	used,was
R14540	T18668	T18669	arg1Of	used,as
R14541	T18671	T18669	arg2Of	control,as
R14542	T18671	T18670	arg1Of	control,a
R14543	T18672	T18673	arg1Of	Sizes,of
R14544	T18672	T18676	arg1Of	Sizes,are
R14545	T18672	T18677	arg2Of	Sizes,indicated.
R14546	T18675	T18673	arg2Of	transcripts,of
R14547	T18675	T18674	arg1Of	transcripts,mRNA
R14548	T18677	T18676	arg2Of	indicated.,are
R14549	T18679	T18678	arg2Of	B,(
R14550	T18680	T18678	arg3Of	),(
R14551	T18681	T18677	arg3Of	Quantification,indicated.
R14552	T18681	T18678	arg1Of	Quantification,(
R14553	T18681	T18682	arg1Of	Quantification,of
R14554	T18681	T18686	arg1Of	Quantification,by
R14555	T18685	T18682	arg2Of	amounts,of
R14556	T18685	T18683	arg1Of	amounts,relative
R14557	T18685	T18684	arg1Of	amounts,mRNA
R14558	T18688	T18687	arg1Of	analysis.,phosphorimager
R14559	T18688	T18689	arg1Of	analysis.,(
R14560	T18690	T18689	arg2Of	C,(
R14561	T18691	T18689	arg3Of	),(
R14562	T18692	T18686	arg2Of	Kinetics,by
R14563	T18692	T18688	arg1Of	Kinetics,analysis.
R14564	T18692	T18693	arg1Of	Kinetics,of
R14565	T18695	T18693	arg2Of	expression,of
R14566	T18695	T18694	arg1Of	expression,protein
R14567	T18695	T18696	arg1Of	expression,in
R14568	T18701	T18696	arg2Of	cells,in
R14569	T18701	T18697	arg1Of	cells,differentiating
R14570	T18701	T18698	arg1Of	cells,P14
R14571	T18701	T18699	arg1Of	cells,CD8+
R14572	T18701	T18700	arg1Of	cells,T
R14573	T18701	T18702	arg2Of	cells,analyzed
R14574	T18704	T18702	arg1Of	immunoblotting,analyzed
R14575	T18704	T18703	arg2Of	immunoblotting,by
R14576	T18705	T18706	arg1Of	Sizes,of
R14577	T18705	T18709	arg1Of	Sizes,are
R14578	T18705	T18710	arg2Of	Sizes,indicated.
R14579	T18708	T18706	arg2Of	bands,of
R14580	T18708	T18707	arg1Of	bands,protein
R14581	T18710	T18709	arg2Of	indicated.,are
R14582	T18712	T18711	arg2Of	D,(
R14583	T18713	T18711	arg3Of	),(
R14584	T18716	T18710	arg3Of	amounts,indicated.
R14585	T18716	T18711	arg1Of	amounts,(
R14586	T18716	T18714	arg1Of	amounts,Relative
R14587	T18716	T18715	arg1Of	amounts,protein
R14588	T18716	T18717	arg2Of	amounts,quantified
R14589	T18716	T18727	arg1Of	amounts,for
R14590	T18717	T18718	arg1Of	quantified,from
R14591	T18723	T18722	arg2Of	E,(
R14592	T18724	T18722	arg3Of	),(
R14593	T18726	T18718	arg2Of	staining,from
R14594	T18726	T18719	arg1Of	staining,the
R14595	T18726	T18720	arg1Of	staining,Western
R14596	T18726	T18721	arg1Of	staining,blots.
R14597	T18726	T18722	arg1Of	staining,(
R14598	T18726	T18725	arg1Of	staining,Intracellular
R14599	T18729	T18728	arg1Of	B,granzyme
R14600	T18729	T18730	arg1Of	B,","
R14601	T18730	T18733	arg1Of	",",and
R14602	T18731	T18730	arg2Of	IFN-γ,","
R14603	T18733	T18727	arg2Of	and,for
R14604	T18733	T18732	arg1Of	and,","
R14605	T18734	T18733	arg2Of	TNF,and
R14606	T18737	T18735	arg1Of	staining,Granzyme
R14607	T18737	T18736	arg1Of	staining,B
R14608	T18737	T18738	arg1Of	staining,was
R14609	T18737	T18739	arg1Of	staining,specific
R14610	T18739	T18738	arg2Of	specific,was
R14611	T18739	T18741	arg1Of	specific,to
R14612	T18741	T18740	arg1Of	to,relative
R14613	T18744	T18741	arg2Of	control,to
R14614	T18744	T18742	arg1Of	control,an
R14615	T18744	T18743	arg1Of	control,isotype
R14616	T18744	T18745	arg1Of	control,(
R14617	T18747	T18745	arg2Of	depicted,(
R14618	T18747	T18746	arg1Of	depicted,not
R14619	T18748	T18745	arg3Of	),(
R14620	T18749	T18750	arg1Of	Cells,were
R14621	T18749	T18751	arg2Of	Cells,restimulated
R14622	T18751	T18750	arg2Of	restimulated,were
R14623	T18751	T18752	arg1Of	restimulated,with
R14624	T18753	T18754	arg1Of	PMA,and
R14625	T18754	T18752	arg2Of	and,with
R14626	T18754	T18756	arg1Of	and,for
R14627	T18755	T18754	arg2Of	ionomycin,and
R14628	T18758	T18757	arg1Of	h.,4
R14629	T18758	T18759	arg1Of	h.,(
R14630	T18760	T18759	arg2Of	F,(
R14631	T18761	T18759	arg3Of	),(
R14632	T18762	T18758	arg1Of	FACS-based,h.
R14633	T18763	T18756	arg2Of	assay,for
R14634	T18763	T18762	arg1Of	assay,FACS-based
R14635	T18763	T18764	modOf	assay,to
R14636	T18765	T18764	arg1Of	measure,to
R14637	T18765	T18773	arg1Of	measure,against
R14638	T18767	T18765	arg2Of	activity,measure
R14639	T18767	T18766	arg1Of	activity,cytolytic
R14640	T18767	T18768	arg1Of	activity,of
R14641	T18772	T18768	arg2Of	cells,of
R14642	T18772	T18769	arg1Of	cells,P14
R14643	T18772	T18770	arg1Of	cells,CD8+
R14644	T18772	T18771	arg1Of	cells,T
R14645	T18775	T18773	arg2Of	targets,against
R14646	T18775	T18774	arg1Of	targets,EL4
R14647	T18775	T18776	arg2Of	targets,loaded
R14648	T18776	T18777	arg1Of	loaded,with
R14649	T18778	T18779	arg1Of	0,(
R14650	T18778	T18782	arg1Of	0,or
R14651	T18780	T18779	arg2Of	−,(
R14652	T18781	T18779	arg3Of	),(
R14653	T18782	T18777	arg2Of	or,with
R14654	T18783	T18782	arg2Of	1,or
R14655	T18783	T18784	arg1Of	1,(
R14656	T18785	T18784	arg2Of	+,(
R14657	T18786	T18784	arg3Of	),(
R14658	T18789	T18765	arg3Of	peptide,measure
R14659	T18789	T18787	arg1Of	peptide,μM
R14660	T18789	T18788	arg1Of	peptide,Gp33
R14661	T18789	T18790	arg1Of	peptide,(
R14662	T18792	T18790	arg2Of	ratio,(
R14663	T18792	T18791	arg1Of	ratio,effector-to-target
R14664	T18792	T18795	arg1Of	ratio,:
R14665	T18795	T18793	arg1Of	:,=
R14666	T18795	T18794	arg1Of	:,5
R14667	T18795	T18796	arg1Of	:,1
R14668	T18797	T18790	arg3Of	),(
R14669	T18798	T18799	arg1Of	Percentage,of
R14670	T18798	T18807	arg1Of	Percentage,in
R14671	T18798	T18817	arg1Of	Percentage,was
R14672	T18798	T18818	arg2Of	Percentage,determined
R14673	T18806	T18799	arg2Of	cells,of
R14674	T18806	T18800	arg1Of	cells,Annexin
R14675	T18806	T18801	arg1Of	cells,V+
R14676	T18806	T18802	arg1Of	cells,(
R14677	T18806	T18803	arg1Of	cells,apoptotic
R14678	T18806	T18804	arg1Of	cells,)
R14679	T18806	T18805	arg1Of	cells,target
R14680	T18812	T18807	arg2Of	population,in
R14681	T18812	T18808	arg1Of	population,the
R14682	T18812	T18809	arg1Of	population,CD8-negative
R14683	T18812	T18810	arg1Of	population,EL4
R14684	T18812	T18811	arg1Of	population,target
R14685	T18812	T18813	arg1Of	population,(
R14686	T18815	T18813	arg2Of	plots,(
R14687	T18815	T18814	arg1Of	plots,dot
R14688	T18816	T18813	arg3Of	),(
R14689	T18818	T18817	arg2Of	determined,was
R14690	T18818	T18819	arg1Of	determined,(
R14691	T18820	T18819	arg2Of	histograms,(
R14692	T18821	T18819	arg3Of	),(
R14693	T18823	T18822	arg1Of	activity,Cytolytic
R14694	T18823	T18824	arg1Of	activity,was
R14695	T18823	T18825	arg2Of	activity,blocked
R14696	T18825	T18824	arg2Of	blocked,was
R14697	T18825	T18826	arg1Of	blocked,by
R14698	T18825	T18836	arg1Of	blocked,","
R14699	T18825	T18837	modOf	blocked,confirming
R14700	T18827	T18826	arg2Of	incubation,by
R14701	T18827	T18828	arg1Of	incubation,with
R14702	T18829	T18830	arg1Of	2,mM
R14703	T18831	T18828	arg2Of	EGTA,with
R14704	T18831	T18829	arg1Of	EGTA,2
R14705	T18831	T18832	arg1Of	EGTA,(
R14706	T18834	T18832	arg2Of	depicted,(
R14707	T18834	T18833	arg1Of	depicted,not
R14708	T18835	T18832	arg3Of	),(
R14709	T18838	T18837	arg2Of	involvement,confirming
R14710	T18838	T18839	arg1Of	involvement,of
R14711	T18842	T18841	arg1Of	exocytosis,granule
R14712	T18842	T18843	arg1Of	exocytosis,(
R14713	T18845	T18843	arg2Of	B,(
R14714	T18845	T18844	arg1Of	B,perforin–granzyme
R14715	T18846	T18843	arg3Of	),(
R14716	T18847	T18839	arg2Of	pathway,of
R14717	T18847	T18840	arg1Of	pathway,the
R14718	T18847	T18842	arg1Of	pathway,exocytosis
R14719	T18848	T18849	arg1Of	Data,are
R14720	T18848	T18850	arg1Of	Data,representative
R14721	T18850	T18849	arg2Of	representative,are
R14722	T18850	T18851	arg1Of	representative,of
R14723	T18854	T18852	arg1Of	five,at
R14724	T18854	T18853	arg1Of	five,least
R14725	T18856	T18855	arg2Of	A–E,(
R14726	T18856	T18858	arg1Of	A–E,or
R14727	T18857	T18855	arg3Of	),(
R14728	T18859	T18858	arg2Of	three,or
R14729	T18861	T18860	arg2Of	F,(
R14730	T18862	T18860	arg3Of	),(
R14731	T18864	T18851	arg2Of	experiments,of
R14732	T18864	T18854	arg1Of	experiments,five
R14733	T18864	T18855	arg1Of	experiments,(
R14734	T18864	T18859	arg1Of	experiments,three
R14735	T18864	T18860	arg1Of	experiments,(
R14736	T18864	T18863	arg1Of	experiments,independent

bionlp-st-ge-2016-coref

Id	Subject	Object	Predicate	Lexical cue
T4255	353-356	Anaphor	denotes	its
T4256	386-394	Antecedent	denotes	perforin
T4257	974-987	Anaphor	denotes	this capacity
T4258	904-913	Antecedent	denotes	expressed
R3444	T4255	T4256	boundBy	its,perforin
R3445	T4257	T4258	boundBy	this capacity,expressed

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T3380	0-8	JJ	denotes	Distinct
T3381	9-19	NN	denotes	expression
T3382	20-28	NNS	denotes	kinetics
T3383	29-31	IN	denotes	of
T3384	32-40	NN	denotes	perforin
T3385	41-44	CC	denotes	and
T3386	45-53	NN	denotes	granzyme
T3387	54-55	NN	denotes	B
T3388	56-62	IN	denotes	during
T3389	63-66	NNP	denotes	CTL
T3390	67-78	NN	denotes	development
T3391	79-81	IN	denotes	in
T3392	82-89	NN	denotes	culture
T3393	90-93	PRP$	denotes	Our
T3394	94-105	NNS	denotes	experiments
T3395	106-114	VBD	denotes	revealed
T3396	115-120	JJ	denotes	clear
T3397	121-132	NNS	denotes	differences
T3398	133-135	IN	denotes	in
T3399	136-139	DT	denotes	the
T3400	140-148	NNS	denotes	kinetics
T3401	149-151	IN	denotes	of
T3402	152-160	NN	denotes	perforin
T3403	160-161	,	denotes	,
T3404	162-170	NN	denotes	granzyme
T3405	171-172	NNP	denotes	B
T3406	172-173	,	denotes	,
T3407	174-177	CC	denotes	and
T3408	178-186	NN	denotes	cytokine
T3409	187-197	NN	denotes	expression
T3410	198-204	IN	denotes	during
T3411	205-208	NNP	denotes	CD8
T3412	208-209	NNP	denotes	+
T3413	210-211	NNP	denotes	T
T3414	212-216	NN	denotes	cell
T3415	217-227	NN	denotes	activation
T3416	228-229	-LRB-	denotes	(
T3417	229-233	FW	denotes	Fig.
T3418	234-235	LS	denotes	1
T3419	235-236	-RRB-	denotes	)
T3420	236-237	.	denotes	.
T3421	238-243	JJ	denotes	Naive
T3422	244-245	NN	denotes	T
T3423	246-251	NNS	denotes	cells
T3424	252-258	VBD	denotes	showed
T3425	259-269	JJ	denotes	detectable
T3426	270-280	NN	denotes	expression
T3427	281-283	IN	denotes	of
T3428	284-292	NN	denotes	perforin
T3429	293-297	NNP	denotes	mRNA
T3430	298-300	RB	denotes	as
T3431	301-305	RB	denotes	well
T3432	306-308	IN	denotes	as
T3433	309-317	NN	denotes	perforin
T3434	318-325	NN	denotes	protein
T3435	326-327	-LRB-	denotes	(
T3436	327-331	FW	denotes	Fig.
T3437	332-333	CD	denotes	1
T3438	333-334	,	denotes	,
T3439	335-336	DT	denotes	A
T3440	337-338	NNP	denotes	D
T3441	338-339	-RRB-	denotes	)
T3442	339-340	.	denotes	.
T3443	341-349	JJ	denotes	Relative
T3444	350-352	TO	denotes	to
T3445	353-356	PRP$	denotes	its
T3446	357-367	NN	denotes	expression
T3447	368-370	IN	denotes	in
T3448	371-376	JJ	denotes	naive
T3449	377-378	NNP	denotes	T
T3450	379-384	NNS	denotes	cells
T3451	384-385	,	denotes	,
T3452	386-394	NN	denotes	perforin
T3453	395-396	-LRB-	denotes	(
T3454	396-400	NNP	denotes	Prf1
T3455	400-401	-RRB-	denotes	)
T3456	402-406	NNP	denotes	mRNA
T3457	407-417	NN	denotes	expression
T3458	418-421	VBD	denotes	did
T3459	422-425	RB	denotes	not
T3460	426-434	VB	denotes	increase
T3461	435-446	RB	denotes	appreciably
T3462	447-449	IN	denotes	at
T3463	450-453	NN	denotes	day
T3464	454-455	CD	denotes	2
T3465	456-459	CC	denotes	but
T3466	460-466	VBD	denotes	showed
T3467	467-468	DT	denotes	a
T3468	469-481	JJ	denotes	reproducible
T3469	482-490	NN	denotes	decrease
T3470	491-493	IN	denotes	at
T3471	494-497	NN	denotes	day
T3472	498-499	CD	denotes	4
T3473	499-500	,	denotes	,
T3474	501-509	VBN	denotes	followed
T3475	510-512	IN	denotes	by
T3476	513-519	JJ	denotes	robust
T3477	520-532	NN	denotes	reexpression
T3478	533-540	IN	denotes	between
T3479	541-545	NNS	denotes	days
T3480	546-547	CD	denotes	4
T3481	548-551	CC	denotes	and
T3482	552-553	CD	denotes	8
T3483	554-555	-LRB-	denotes	(
T3484	555-559	NN	denotes	Fig.
T3485	560-561	CD	denotes	1
T3486	561-562	,	denotes	,
T3487	563-564	DT	denotes	A
T3488	565-566	NNP	denotes	D
T3489	566-567	-RRB-	denotes	)
T3490	567-568	.	denotes	.
T3491	569-571	IN	denotes	In
T3492	572-580	NN	denotes	contrast
T3493	580-581	,	denotes	,
T3494	582-590	NN	denotes	granzyme
T3495	591-592	NNP	denotes	B
T3496	593-594	-LRB-	denotes	(
T3497	594-598	NNP	denotes	Gzmb
T3498	598-599	-RRB-	denotes	)
T3499	600-604	NNP	denotes	mRNA
T3500	605-608	VBD	denotes	was
T3501	609-612	JJ	denotes	low
T3502	613-615	CC	denotes	or
T3503	616-628	JJ	denotes	undetectable
T3504	629-631	IN	denotes	in
T3505	632-637	JJ	denotes	naive
T3506	638-639	NNP	denotes	T
T3507	640-645	NNS	denotes	cells
T3508	646-649	CC	denotes	but
T3509	650-653	VBD	denotes	was
T3510	654-662	RB	denotes	strongly
T3511	663-675	JJ	denotes	up-regulated
T3512	676-678	IN	denotes	by
T3513	679-682	NN	denotes	day
T3514	683-684	CD	denotes	2
T3515	685-690	IN	denotes	after
T3516	691-702	NN	denotes	stimulation
T3517	703-706	CC	denotes	and
T3518	707-716	VBD	denotes	increased
T3519	717-730	RB	denotes	progressively
T3520	731-736	IN	denotes	until
T3521	737-740	NN	denotes	day
T3522	741-742	CD	denotes	6
T3523	743-744	-LRB-	denotes	(
T3524	744-748	NN	denotes	Fig.
T3525	749-750	CD	denotes	1
T3526	750-751	,	denotes	,
T3527	752-753	DT	denotes	A
T3528	754-757	CC	denotes	and
T3529	758-759	NNP	denotes	B
T3530	759-760	-RRB-	denotes	)
T3531	760-761	:	denotes	;
T3532	762-771	RB	denotes	similarly
T3533	771-772	,	denotes	,
T3534	773-781	NN	denotes	granzyme
T3535	782-783	NNP	denotes	B
T3536	784-791	NN	denotes	protein
T3537	792-795	VBD	denotes	was
T3538	796-805	VBN	denotes	expressed
T3539	806-808	IN	denotes	by
T3540	809-812	NN	denotes	day
T3541	813-814	CD	denotes	4
T3542	815-818	CC	denotes	and
T3543	819-827	VBD	denotes	remained
T3544	828-832	JJ	denotes	high
T3545	833-838	IN	denotes	until
T3546	839-842	NN	denotes	day
T3547	843-844	CD	denotes	6
T3548	845-846	-LRB-	denotes	(
T3549	846-850	NN	denotes	Fig.
T3550	851-852	CD	denotes	1
T3551	853-854	NNP	denotes	E
T3552	854-855	-RRB-	denotes	)
T3553	855-856	.	denotes	.
T3554	857-859	IN	denotes	As
T3555	860-868	VBN	denotes	expected
T3556	868-869	,	denotes	,
T3557	870-871	DT	denotes	a
T3558	872-877	JJ	denotes	small
T3559	878-886	NN	denotes	fraction
T3560	887-889	IN	denotes	of
T3561	890-895	JJ	denotes	naive
T3562	896-897	NNP	denotes	T
T3563	898-903	NNS	denotes	cells
T3564	904-913	VBD	denotes	expressed
T3565	914-917	DT	denotes	the
T3566	918-927	NNS	denotes	cytokines
T3567	928-933	NNP	denotes	IFN-γ
T3568	934-937	CC	denotes	and
T3569	938-941	NNP	denotes	TNF
T3570	942-944	IN	denotes	in
T3571	945-953	NN	denotes	response
T3572	954-956	TO	denotes	to
T3573	957-968	NN	denotes	stimulation
T3574	968-969	,	denotes	,
T3575	970-973	CC	denotes	and
T3576	974-978	DT	denotes	this
T3577	979-987	NN	denotes	capacity
T3578	988-997	VBD	denotes	increased
T3579	998-1011	RB	denotes	significantly
T3580	1012-1014	IN	denotes	in
T3581	1015-1029	JJ	denotes	differentiated
T3582	1030-1035	NNS	denotes	cells
T3583	1036-1037	-LRB-	denotes	(
T3584	1037-1041	NNP	denotes	Fig.
T3585	1042-1043	CD	denotes	1
T3586	1044-1045	NNP	denotes	E
T3587	1045-1046	:	denotes	;
T3588	1047-1050	VB	denotes	see
T3589	1051-1055	RB	denotes	also
T3590	1056-1060	JJ	denotes	Fig.
T3591	1061-1062	CD	denotes	2
T3592	1063-1064	NN	denotes	A
T3593	1064-1065	-RRB-	denotes	)
T3594	1065-1066	.	denotes	.
T3595	2375-2377	PRP	denotes	We
T3596	2378-2387	VBD	denotes	evaluated
T3597	2388-2405	JJ	denotes	antigen-dependent
T3598	2406-2415	JJ	denotes	cytolytic
T3599	2416-2424	NN	denotes	function
T3600	2425-2427	IN	denotes	in
T3601	2428-2429	DT	denotes	a
T3602	2430-2440	JJ	denotes	short-term
T3603	2441-2446	NN	denotes	assay
T3604	2447-2449	IN	denotes	in
T3605	2450-2455	WDT	denotes	which
T3606	2456-2462	NN	denotes	target
T3607	2463-2467	NN	denotes	cell
T3608	2468-2473	NN	denotes	death
T3609	2474-2477	VBD	denotes	was
T3610	2478-2486	VBN	denotes	measured
T3611	2487-2493	IN	denotes	within
T3612	2494-2495	CD	denotes	2
T3613	2496-2497	NN	denotes	h
T3614	2498-2499	-LRB-	denotes	(
T3615	2499-2503	FW	denotes	Fig.
T3616	2504-2505	CD	denotes	1
T3617	2506-2507	NN	denotes	F
T3618	2507-2508	-RRB-	denotes	)
T3619	2508-2509	.	denotes	.
T3620	2510-2512	IN	denotes	By
T3621	2513-2521	VBG	denotes	limiting
T3622	2522-2525	DT	denotes	the
T3623	2526-2534	NN	denotes	duration
T3624	2535-2537	IN	denotes	of
T3625	2538-2541	NNP	denotes	TCR
T3626	2542-2553	NN	denotes	stimulation
T3627	2553-2554	,	denotes	,
T3628	2555-2559	DT	denotes	this
T3629	2560-2568	NN	denotes	strategy
T3630	2569-2578	VBZ	denotes	minimizes
T3631	2579-2588	NN	denotes	cytolysis
T3632	2589-2598	JJ	denotes	secondary
T3633	2599-2601	TO	denotes	to
T3634	2602-2605	JJ	denotes	new
T3635	2606-2610	NN	denotes	gene
T3636	2611-2621	NN	denotes	expression
T3637	2622-2628	IN	denotes	during
T3638	2629-2632	DT	denotes	the
T3639	2633-2639	NN	denotes	period
T3640	2640-2642	IN	denotes	of
T3641	2643-2646	DT	denotes	the
T3642	2647-2652	NN	denotes	assay
T3643	2652-2653	.	denotes	.
T3644	2654-2659	JJ	denotes	Naive
T3645	2660-2661	NN	denotes	T
T3646	2662-2667	NNS	denotes	cells
T3647	2668-2671	VBD	denotes	did
T3648	2672-2675	RB	denotes	not
T3649	2676-2683	NN	denotes	display
T3650	2684-2695	JJ	denotes	significant
T3651	2696-2705	JJ	denotes	cytolytic
T3652	2706-2714	NN	denotes	function
T3653	2715-2717	IN	denotes	in
T3654	2718-2722	DT	denotes	this
T3655	2723-2733	JJ	denotes	short-term
T3656	2734-2739	NN	denotes	assay
T3657	2740-2741	-LRB-	denotes	(
T3658	2741-2752	JJ	denotes	unpublished
T3659	2753-2757	NNS	denotes	data
T3660	2757-2758	-RRB-	denotes	)
T3661	2758-2759	,	denotes	,
T3662	2760-2764	RBS	denotes	most
T3663	2765-2771	JJ	denotes	likely
T3664	2772-2779	IN	denotes	because
T3665	2780-2784	PRP	denotes	they
T3666	2785-2792	VBP	denotes	express
T3667	2793-2801	NN	denotes	immature
T3668	2802-2803	-LRB-	denotes	(
T3669	2803-2814	JJ	denotes	unprocessed
T3670	2814-2815	-RRB-	denotes	)
T3671	2816-2821	NNS	denotes	forms
T3672	2822-2824	IN	denotes	of
T3673	2825-2833	NN	denotes	perforin
T3674	2834-2837	CC	denotes	and
T3675	2838-2842	VBP	denotes	lack
T3676	2843-2846	DT	denotes	the
T3677	2847-2855	NN	denotes	capacity
T3678	2856-2858	TO	denotes	to
T3679	2859-2870	VB	denotes	degranulate
T3680	2871-2872	-LRB-	denotes	(
T3681	2872-2874	CD	denotes	18
T3682	2874-2875	,	denotes	,
T3683	2876-2878	CD	denotes	19
T3684	2878-2879	-RRB-	denotes	)
T3685	2879-2880	.	denotes	.
T3686	2881-2885	RB	denotes	Even
T3687	2886-2891	IN	denotes	after
T3688	2892-2902	NN	denotes	activation
T3689	2903-2906	IN	denotes	for
T3690	2907-2908	CD	denotes	2
T3691	2909-2911	CC	denotes	or
T3692	2912-2913	CD	denotes	4
T3693	2914-2915	LS	denotes	d
T3694	2915-2916	,	denotes	,
T3695	2917-2920	DT	denotes	the
T3696	2921-2926	NNS	denotes	cells
T3697	2927-2933	VBD	denotes	showed
T3698	2934-2938	JJ	denotes	poor
T3699	2939-2948	JJ	denotes	cytolytic
T3700	2949-2957	NN	denotes	activity
T3701	2958-2959	-LRB-	denotes	(
T3702	2959-2963	FW	denotes	Fig.
T3703	2964-2965	CD	denotes	1
T3704	2966-2967	NN	denotes	F
T3705	2967-2968	-RRB-	denotes	)
T3706	2968-2969	,	denotes	,
T3707	2970-2972	IN	denotes	in
T3708	2973-2981	JJ	denotes	striking
T3709	2982-2990	NN	denotes	contrast
T3710	2991-2993	TO	denotes	to
T3711	2994-2999	PRP$	denotes	their
T3712	3000-3008	NN	denotes	capacity
T3713	3009-3012	IN	denotes	for
T3714	3013-3022	JJ	denotes	efficient
T3715	3023-3031	NN	denotes	cytokine
T3716	3032-3042	NN	denotes	production
T3717	3043-3044	-LRB-	denotes	(
T3718	3044-3048	FW	denotes	Fig.
T3719	3049-3050	CD	denotes	1
T3720	3051-3052	NNP	denotes	E
T3721	3052-3053	-RRB-	denotes	)
T3722	3053-3054	.	denotes	.
T3723	3055-3059	RB	denotes	Only
T3724	3060-3065	NNS	denotes	cells
T3725	3066-3074	VBD	denotes	cultured
T3726	3075-3080	IN	denotes	until
T3727	3081-3084	NN	denotes	day
T3728	3085-3086	CD	denotes	6
T3729	3087-3096	VBD	denotes	displayed
T3730	3097-3103	JJ	denotes	robust
T3731	3104-3116	NN	denotes	cytotoxicity
T3732	3116-3117	,	denotes	,
T3733	3118-3120	IN	denotes	as
T3734	3121-3127	VBN	denotes	judged
T3735	3128-3130	IN	denotes	by
T3736	3131-3136	PRP$	denotes	their
T3737	3137-3144	NN	denotes	ability
T3738	3145-3147	TO	denotes	to
T3739	3148-3154	VB	denotes	induce
T3740	3155-3164	NN	denotes	apoptosis
T3741	3165-3167	IN	denotes	in
T3742	3168-3169	DT	denotes	a
T3743	3170-3175	JJ	denotes	large
T3744	3176-3182	NN	denotes	number
T3745	3183-3185	IN	denotes	of
T3746	3186-3192	NN	denotes	target
T3747	3193-3198	NNS	denotes	cells
T3748	3199-3200	-LRB-	denotes	(
T3749	3200-3204	NNP	denotes	Fig.
T3750	3205-3206	CD	denotes	1
T3751	3207-3208	NN	denotes	F
T3752	3208-3209	-RRB-	denotes	)
T3753	3209-3210	.	denotes	.
T3754	3211-3216	DT	denotes	These
T3755	3217-3224	NNS	denotes	results
T3756	3225-3229	VBP	denotes	show
T3757	3230-3234	IN	denotes	that
T3758	3235-3240	IN	denotes	after
T3759	3241-3242	DT	denotes	a
T3760	3243-3249	JJ	denotes	strong
T3761	3250-3257	NN	denotes	priming
T3762	3258-3266	NN	denotes	stimulus
T3763	3267-3274	IN	denotes	through
T3764	3275-3279	NNS	denotes	TCRs
T3765	3280-3283	CC	denotes	and
T3766	3284-3298	JJ	denotes	co-stimulatory
T3767	3299-3308	NNS	denotes	receptors
T3768	3309-3311	IN	denotes	in
T3769	3312-3317	NN	denotes	vitro
T3770	3317-3318	,	denotes	,
T3771	3319-3327	NN	denotes	granzyme
T3772	3328-3329	NNP	denotes	B
T3773	3330-3340	NN	denotes	expression
T3774	3341-3344	CC	denotes	and
T3775	3345-3348	DT	denotes	the
T3776	3349-3356	NN	denotes	ability
T3777	3357-3359	TO	denotes	to
T3778	3360-3367	VB	denotes	produce
T3779	3368-3376	NN	denotes	effector
T3780	3377-3386	NNS	denotes	cytokines
T3781	3387-3390	VBP	denotes	are
T3782	3391-3401	VBN	denotes	programmed
T3783	3402-3407	RB	denotes	early
T3784	3407-3408	,	denotes	,
T3785	3409-3416	IN	denotes	whereas
T3786	3417-3425	NN	denotes	perforin
T3787	3426-3436	NN	denotes	expression
T3788	3437-3440	CC	denotes	and
T3789	3441-3450	JJ	denotes	cytolytic
T3790	3451-3459	NN	denotes	function
T3791	3460-3463	VBP	denotes	are
T3792	3464-3471	JJ	denotes	induced
T3793	3472-3477	RB	denotes	later
T3794	3477-3478	,	denotes	,
T3795	3479-3485	IN	denotes	during
T3796	3486-3489	DT	denotes	the
T3797	3490-3495	NN	denotes	phase
T3798	3496-3498	IN	denotes	of
T3799	3499-3505	JJ	denotes	clonal
T3800	3506-3515	NN	denotes	expansion
T3801	3516-3518	IN	denotes	in
T3802	3519-3523	NN	denotes	IL-2
T3803	3523-3524	.	denotes	.
T3804	3525-3534	RB	denotes	Therefore
T3805	3534-3535	,	denotes	,
T3806	3536-3539	DT	denotes	the
T3807	3540-3543	CD	denotes	two
T3808	3544-3549	JJ	denotes	major
T3809	3550-3558	NN	denotes	effector
T3810	3559-3568	NNS	denotes	functions
T3811	3569-3571	IN	denotes	of
T3812	3572-3575	NNP	denotes	CTL
T3813	3575-3576	,	denotes	,
T3814	3577-3585	NN	denotes	cytokine
T3815	3586-3596	NN	denotes	production
T3816	3597-3600	CC	denotes	and
T3817	3601-3610	JJ	denotes	cytolytic
T3818	3611-3619	NN	denotes	activity
T3819	3619-3620	,	denotes	,
T3820	3621-3624	VBP	denotes	are
T3821	3625-3628	RB	denotes	not
T3822	3629-3642	RB	denotes	intrinsically
T3823	3643-3654	VBN	denotes	coregulated
T3824	3654-3655	.	denotes	.
T18364	1077-1085	NNS	denotes	Kinetics
T18365	1086-1088	IN	denotes	of
T18366	1089-1093	NN	denotes	gene
T18367	1094-1104	NN	denotes	expression
T18368	1105-1111	IN	denotes	during
T18369	1112-1115	NNP	denotes	CD8
T18370	1115-1116	NNP	denotes	+
T18371	1117-1118	NNP	denotes	T
T18372	1119-1123	NN	denotes	cell
T18373	1124-1139	NN	denotes	differentiation
T18374	1139-1140	.	denotes	.
T18375	1141-1142	-LRB-	denotes	(
T18376	1142-1143	NNP	denotes	A
T18377	1143-1144	-RRB-	denotes	)
T18378	1145-1153	NNPS	denotes	Kinetics
T18379	1154-1156	IN	denotes	of
T18380	1157-1161	NNP	denotes	Prf1
T18381	1161-1162	,	denotes	,
T18382	1163-1167	NNP	denotes	Gzmb
T18383	1167-1168	,	denotes	,
T18384	1169-1174	NNP	denotes	Tbx21
T18385	1175-1176	-LRB-	denotes	(
T18386	1176-1181	NNP	denotes	T-bet
T18387	1181-1182	-RRB-	denotes	)
T18388	1182-1183	,	denotes	,
T18389	1184-1187	CC	denotes	and
T18390	1188-1193	NNP	denotes	Eomes
T18391	1194-1198	NNP	denotes	mRNA
T18392	1199-1209	NN	denotes	expression
T18393	1210-1212	IN	denotes	in
T18394	1213-1228	VBG	denotes	differentiating
T18395	1229-1232	NNP	denotes	P14
T18396	1233-1236	NNP	denotes	CD8
T18397	1236-1237	VBD	denotes	+
T18398	1238-1239	NNP	denotes	T
T18399	1240-1245	NNS	denotes	cells
T18400	1246-1254	VBD	denotes	analyzed
T18401	1255-1257	IN	denotes	by
T18402	1258-1266	NNP	denotes	Northern
T18403	1267-1275	VBG	denotes	blotting
T18404	1275-1276	.	denotes	.
T18405	1277-1280	NNP	denotes	RNA
T18406	1281-1285	IN	denotes	from
T18407	1286-1289	NN	denotes	day
T18408	1290-1291	CD	denotes	7
T18409	1292-1295	CD	denotes	Th1
T18410	1296-1301	NNS	denotes	cells
T18411	1302-1305	VBD	denotes	was
T18412	1306-1310	VBN	denotes	used
T18413	1311-1313	IN	denotes	as
T18414	1314-1315	DT	denotes	a
T18415	1316-1323	NN	denotes	control
T18416	1323-1324	.	denotes	.
T18417	1325-1330	NNS	denotes	Sizes
T18418	1331-1333	IN	denotes	of
T18419	1334-1338	NNP	denotes	mRNA
T18420	1339-1350	NNS	denotes	transcripts
T18421	1351-1354	VBP	denotes	are
T18422	1355-1364	VBN	denotes	indicated
T18423	1364-1365	.	denotes	.
T18424	1366-1367	-LRB-	denotes	(
T18425	1367-1368	NNP	denotes	B
T18426	1368-1369	-RRB-	denotes	)
T18427	1370-1384	NN	denotes	Quantification
T18428	1385-1387	IN	denotes	of
T18429	1388-1396	JJ	denotes	relative
T18430	1397-1401	NNP	denotes	mRNA
T18431	1402-1409	NNS	denotes	amounts
T18432	1410-1412	IN	denotes	by
T18433	1413-1427	NN	denotes	phosphorimager
T18434	1428-1436	NN	denotes	analysis
T18435	1436-1437	.	denotes	.
T18436	1438-1439	-LRB-	denotes	(
T18437	1439-1440	$	denotes	C
T18438	1440-1441	-RRB-	denotes	)
T18439	1442-1450	NNPS	denotes	Kinetics
T18440	1451-1453	IN	denotes	of
T18441	1454-1461	NN	denotes	protein
T18442	1462-1472	NN	denotes	expression
T18443	1473-1475	IN	denotes	in
T18444	1476-1491	VBG	denotes	differentiating
T18445	1492-1495	NNP	denotes	P14
T18446	1496-1499	NNP	denotes	CD8
T18447	1499-1500	VBD	denotes	+
T18448	1501-1502	NNP	denotes	T
T18449	1503-1508	NNS	denotes	cells
T18450	1509-1517	VBD	denotes	analyzed
T18451	1518-1520	IN	denotes	by
T18452	1521-1535	VBG	denotes	immunoblotting
T18453	1535-1536	.	denotes	.
T18454	1537-1542	NNS	denotes	Sizes
T18455	1543-1545	IN	denotes	of
T18456	1546-1553	NN	denotes	protein
T18457	1554-1559	NNS	denotes	bands
T18458	1560-1563	VBP	denotes	are
T18459	1564-1573	VBN	denotes	indicated
T18460	1573-1574	.	denotes	.
T18461	1575-1576	-LRB-	denotes	(
T18462	1576-1577	NNP	denotes	D
T18463	1577-1578	-RRB-	denotes	)
T18464	1579-1587	JJ	denotes	Relative
T18465	1588-1595	NN	denotes	protein
T18466	1596-1603	NNS	denotes	amounts
T18467	1604-1614	VBN	denotes	quantified
T18468	1615-1619	IN	denotes	from
T18469	1620-1623	DT	denotes	the
T18470	1624-1631	JJ	denotes	Western
T18471	1632-1637	NNS	denotes	blots
T18472	1637-1638	.	denotes	.
T18473	1639-1640	-LRB-	denotes	(
T18474	1640-1641	NNP	denotes	E
T18475	1641-1642	-RRB-	denotes	)
T18476	1643-1656	NNP	denotes	Intracellular
T18477	1657-1665	VBG	denotes	staining
T18478	1666-1669	IN	denotes	for
T18479	1670-1678	NN	denotes	granzyme
T18480	1679-1680	NNP	denotes	B
T18481	1680-1681	,	denotes	,
T18482	1682-1687	NNP	denotes	IFN-γ
T18483	1687-1688	,	denotes	,
T18484	1689-1692	CC	denotes	and
T18485	1693-1696	NNP	denotes	TNF
T18486	1696-1697	.	denotes	.
T18487	1698-1706	NNP	denotes	Granzyme
T18488	1707-1708	NNP	denotes	B
T18489	1709-1717	VBG	denotes	staining
T18490	1718-1721	VBD	denotes	was
T18491	1722-1730	JJ	denotes	specific
T18492	1731-1739	JJ	denotes	relative
T18493	1740-1742	TO	denotes	to
T18494	1743-1745	DT	denotes	an
T18495	1746-1753	NN	denotes	isotype
T18496	1754-1761	NN	denotes	control
T18497	1762-1763	-LRB-	denotes	(
T18498	1763-1766	RB	denotes	not
T18499	1767-1775	VBN	denotes	depicted
T18500	1775-1776	-RRB-	denotes	)
T18501	1776-1777	.	denotes	.
T18502	1778-1783	NNS	denotes	Cells
T18503	1784-1788	VBD	denotes	were
T18504	1789-1801	VBN	denotes	restimulated
T18505	1802-1806	IN	denotes	with
T18506	1807-1810	NNP	denotes	PMA
T18507	1811-1814	CC	denotes	and
T18508	1815-1824	NN	denotes	ionomycin
T18509	1825-1828	IN	denotes	for
T18510	1829-1830	CD	denotes	4
T18511	1831-1833	NN	denotes	h.
T18512	1834-1835	-LRB-	denotes	(
T18513	1835-1836	NN	denotes	F
T18514	1836-1837	-RRB-	denotes	)
T18515	1838-1848	JJ	denotes	FACS-based
T18516	1849-1854	NN	denotes	assay
T18517	1855-1857	TO	denotes	to
T18518	1858-1865	VB	denotes	measure
T18519	1866-1875	JJ	denotes	cytolytic
T18520	1876-1884	NN	denotes	activity
T18521	1885-1887	IN	denotes	of
T18522	1888-1891	NNP	denotes	P14
T18523	1892-1895	NNP	denotes	CD8
T18524	1895-1896	VBD	denotes	+
T18525	1897-1898	NNP	denotes	T
T18526	1899-1904	NNS	denotes	cells
T18527	1905-1912	IN	denotes	against
T18528	1913-1916	CD	denotes	EL4
T18529	1917-1924	NNS	denotes	targets
T18530	1925-1931	VBN	denotes	loaded
T18531	1932-1936	IN	denotes	with
T18532	1937-1938	CD	denotes	0
T18533	1939-1940	-LRB-	denotes	(
T18534	1940-1941	NN	denotes	−
T18535	1941-1942	-RRB-	denotes	)
T18536	1943-1945	CC	denotes	or
T18537	1946-1947	CD	denotes	1
T18538	1948-1949	-LRB-	denotes	(
T18539	1949-1950	NN	denotes	+
T18540	1950-1951	-RRB-	denotes	)
T18541	1952-1954	NNP	denotes	μM
T18542	1955-1959	NNP	denotes	Gp33
T18543	1960-1967	NN	denotes	peptide
T18544	1968-1969	-LRB-	denotes	(
T18545	1969-1987	JJ	denotes	effector-to-target
T18546	1988-1993	NN	denotes	ratio
T18547	1994-1995	SYM	denotes	=
T18548	1996-1999	CD	denotes	5:1
T18549	1999-2000	-RRB-	denotes	)
T18550	2000-2001	.	denotes	.
T18551	2002-2012	NN	denotes	Percentage
T18552	2013-2015	IN	denotes	of
T18553	2016-2023	NNP	denotes	Annexin
T18554	2024-2025	NNP	denotes	V
T18555	2025-2026	NN	denotes	+
T18556	2027-2028	-LRB-	denotes	(
T18557	2028-2037	JJ	denotes	apoptotic
T18558	2037-2038	-RRB-	denotes	)
T18559	2039-2045	NN	denotes	target
T18560	2046-2051	NNS	denotes	cells
T18561	2052-2054	IN	denotes	in
T18562	2055-2058	DT	denotes	the
T18563	2059-2071	JJ	denotes	CD8-negative
T18564	2072-2075	CD	denotes	EL4
T18565	2076-2082	NN	denotes	target
T18566	2083-2093	NN	denotes	population
T18567	2094-2095	-LRB-	denotes	(
T18568	2095-2098	VB	denotes	dot
T18569	2099-2104	NNS	denotes	plots
T18570	2104-2105	-RRB-	denotes	)
T18571	2106-2109	VBD	denotes	was
T18572	2110-2120	VBN	denotes	determined
T18573	2121-2122	-LRB-	denotes	(
T18574	2122-2132	NNS	denotes	histograms
T18575	2132-2133	-RRB-	denotes	)
T18576	2133-2134	.	denotes	.
T18577	2135-2144	JJ	denotes	Cytolytic
T18578	2145-2153	NN	denotes	activity
T18579	2154-2157	VBD	denotes	was
T18580	2158-2165	VBN	denotes	blocked
T18581	2166-2168	IN	denotes	by
T18582	2169-2179	NN	denotes	incubation
T18583	2180-2184	IN	denotes	with
T18584	2185-2186	CD	denotes	2
T18585	2187-2189	NNP	denotes	mM
T18586	2190-2194	NNP	denotes	EGTA
T18587	2195-2196	-LRB-	denotes	(
T18588	2196-2199	RB	denotes	not
T18589	2200-2208	VBN	denotes	depicted
T18590	2208-2209	-RRB-	denotes	)
T18591	2209-2210	,	denotes	,
T18592	2211-2221	VBG	denotes	confirming
T18593	2222-2233	NN	denotes	involvement
T18594	2234-2236	IN	denotes	of
T18595	2237-2240	DT	denotes	the
T18596	2241-2248	NN	denotes	granule
T18597	2249-2259	NN	denotes	exocytosis
T18598	2260-2261	-LRB-	denotes	(
T18599	2261-2269	NN	denotes	perforin
T18600	2270-2278	NN	denotes	granzyme
T18601	2279-2280	NN	denotes	B
T18602	2280-2281	-RRB-	denotes	)
T18603	2282-2289	NN	denotes	pathway
T18604	2289-2290	.	denotes	.
T18605	2291-2295	NNS	denotes	Data
T18606	2296-2299	VBP	denotes	are
T18607	2300-2314	NN	denotes	representative
T18608	2315-2317	IN	denotes	of
T18609	2318-2320	IN	denotes	at
T18610	2321-2326	JJS	denotes	least
T18611	2327-2331	CD	denotes	five
T18612	2332-2333	-LRB-	denotes	(
T18613	2333-2334	DT	denotes	A
T18614	2335-2336	NNP	denotes	E
T18615	2336-2337	-RRB-	denotes	)
T18616	2338-2340	CC	denotes	or
T18617	2341-2346	CD	denotes	three
T18618	2347-2348	-LRB-	denotes	(
T18619	2348-2349	NN	denotes	F
T18620	2349-2350	-RRB-	denotes	)
T18621	2351-2362	JJ	denotes	independent
T18622	2363-2374	NNS	denotes	experiments
T18623	2374-2375	.	denotes	.
R2564	T3380	T3382	amod	Distinct,kinetics
R2565	T3381	T3382	compound	expression,kinetics
R2566	T3382	T3395	nsubj	kinetics,revealed
R2567	T3383	T3382	prep	of,kinetics
R2568	T3384	T3387	nmod	perforin,B
R2569	T3385	T3384	cc	and,perforin
R2570	T3386	T3387	compound	granzyme,B
R2571	T3387	T3383	pobj	B,of
R2572	T3388	T3382	prep	during,kinetics
R2573	T3389	T3390	compound	CTL,development
R2574	T3390	T3388	pobj	development,during
R2575	T3391	T3390	prep	in,development
R2576	T3392	T3391	pobj	culture,in
R2577	T3393	T3394	poss	Our,experiments
R2578	T3394	T3395	nsubj	experiments,revealed
R2579	T3395	T3395	ROOT	revealed,revealed
R2580	T3396	T3397	amod	clear,differences
R2581	T3397	T3395	dobj	differences,revealed
R2582	T3398	T3397	prep	in,differences
R2583	T3399	T3400	det	the,kinetics
R2584	T3400	T3398	pobj	kinetics,in
R2585	T3401	T3400	prep	of,kinetics
R2586	T3402	T3401	pobj	perforin,of
R2587	T3403	T3397	punct	",",differences
R2588	T3404	T3405	compound	granzyme,B
R2589	T3405	T3397	appos	B,differences
R2590	T3406	T3405	punct	",",B
R2591	T3407	T3405	cc	and,B
R2592	T3408	T3409	compound	cytokine,expression
R2593	T3409	T3405	conj	expression,B
R2594	T3410	T3397	prep	during,differences
R2595	T3411	T3415	compound	CD8,activation
R2596	T3412	T3415	compound	+,activation
R2597	T3413	T3415	compound	T,activation
R2598	T3414	T3415	compound	cell,activation
R2599	T3415	T3410	pobj	activation,during
R2600	T3416	T3418	punct	(,1
R2601	T3417	T3418	nmod	Fig.,1
R2602	T3418	T3397	parataxis	1,differences
R2603	T3419	T3418	punct	),1
R2604	T3420	T3395	punct	.,revealed
R2605	T3421	T3423	amod	Naive,cells
R2606	T3422	T3423	compound	T,cells
R2607	T3423	T3424	nsubj	cells,showed
R2608	T3424	T3424	ROOT	showed,showed
R2609	T3425	T3426	amod	detectable,expression
R2610	T3426	T3424	dobj	expression,showed
R2611	T3427	T3426	prep	of,expression
R2612	T3428	T3429	compound	perforin,mRNA
R2613	T3429	T3427	pobj	mRNA,of
R2614	T3430	T3432	advmod	as,as
R2615	T3431	T3432	advmod	well,as
R2616	T3432	T3426	cc	as,expression
R2617	T3433	T3434	compound	perforin,protein
R2618	T3434	T3426	conj	protein,expression
R2619	T3435	T3434	punct	(,protein
R2620	T3436	T3434	parataxis	Fig.,protein
R2621	T3437	T3436	nummod	1,Fig.
R2622	T3438	T3436	punct	",",Fig.
R2623	T3439	T3440	det	A,D
R2624	T3440	T3436	appos	D,Fig.
R2625	T3441	T3436	punct	),Fig.
R2626	T3442	T3424	punct	.,showed
R2627	T3443	T3443	ROOT	Relative,Relative
R2628	T3444	T3443	prep	to,Relative
R2629	T3445	T3446	poss	its,expression
R2630	T3446	T3444	pobj	expression,to
R2631	T3447	T3446	prep	in,expression
R2632	T3448	T3450	amod	naive,cells
R2633	T3449	T3450	compound	T,cells
R2634	T3450	T3447	pobj	cells,in
R2635	T3451	T3443	punct	",",Relative
R2636	T3452	T3443	conj	perforin,Relative
R2637	T3453	T3452	punct	(,perforin
R2638	T3454	T3452	appos	Prf1,perforin
R2639	T3455	T3452	punct	),perforin
R2640	T3456	T3457	compound	mRNA,expression
R2641	T3457	T3460	nsubj	expression,increase
R2642	T3458	T3460	aux	did,increase
R2643	T3459	T3460	neg	not,increase
R2644	T3460	T3460	ROOT	increase,increase
R2645	T3461	T3460	advmod	appreciably,increase
R2646	T3462	T3460	prep	at,increase
R2647	T3463	T3462	pobj	day,at
R2648	T3464	T3463	nummod	2,day
R2649	T3465	T3460	cc	but,increase
R2650	T3466	T3460	conj	showed,increase
R2651	T3467	T3469	det	a,decrease
R2652	T3468	T3469	amod	reproducible,decrease
R2653	T3469	T3466	dobj	decrease,showed
R2654	T3470	T3469	prep	at,decrease
R2655	T3471	T3470	pobj	day,at
R2656	T3472	T3471	nummod	4,day
R2657	T3473	T3469	punct	",",decrease
R2658	T3474	T3466	conj	followed,showed
R2659	T3475	T3474	agent	by,followed
R2660	T3476	T3477	amod	robust,reexpression
R2661	T3477	T3475	pobj	reexpression,by
R2662	T3478	T3474	prep	between,followed
R2663	T3479	T3478	pobj	days,between
R2664	T3480	T3484	nummod	4,Fig.
R2665	T3481	T3480	cc	and,4
R2666	T3482	T3480	conj	8,4
R2667	T3483	T3484	punct	(,Fig.
R2668	T3484	T3474	npadvmod	Fig.,followed
R2669	T3485	T3484	nummod	1,Fig.
R2670	T3486	T3484	punct	",",Fig.
R2671	T3487	T3484	appos	A,Fig.
R2672	T3488	T3487	appos	D,A
R2673	T3489	T3487	punct	),A
R2674	T3490	T3460	punct	.,increase
R2675	T3491	T3491	ROOT	In,In
R2676	T3492	T3491	pobj	contrast,In
R2677	T3493	T3495	quantmod	",",B
R2678	T3494	T3495	compound	granzyme,B
R2679	T3495	T3491	conj	B,In
R2680	T3496	T3495	punct	(,B
R2681	T3497	T3495	appos	Gzmb,B
R2682	T3498	T3495	punct	),B
R2683	T3499	T3500	nsubj	mRNA,was
R2684	T3500	T3500	ROOT	was,was
R2685	T3501	T3500	acomp	low,was
R2686	T3502	T3501	cc	or,low
R2687	T3503	T3501	conj	undetectable,low
R2688	T3504	T3503	prep	in,undetectable
R2689	T3505	T3507	amod	naive,cells
R2690	T3506	T3507	compound	T,cells
R2691	T3507	T3504	pobj	cells,in
R2692	T3508	T3500	cc	but,was
R2693	T3509	T3500	conj	was,was
R2694	T3510	T3511	advmod	strongly,up-regulated
R2695	T3511	T3509	acomp	up-regulated,was
R2696	T3512	T3511	prep	by,up-regulated
R2697	T3513	T3512	pobj	day,by
R2698	T3514	T3513	nummod	2,day
R2699	T3515	T3513	prep	after,day
R2700	T3516	T3515	pobj	stimulation,after
R2701	T3517	T3511	cc	and,up-regulated
R2702	T3518	T3500	conj	increased,was
R2703	T3519	T3518	advmod	progressively,increased
R2704	T3520	T3518	prep	until,increased
R2705	T3521	T3520	pobj	day,until
R2706	T3522	T3521	nummod	6,day
R2707	T3523	T3524	punct	(,Fig.
R2708	T3524	T3518	npadvmod	Fig.,increased
R2709	T3525	T3524	nummod	1,Fig.
R2710	T3526	T3524	punct	",",Fig.
R2711	T3527	T3524	conj	A,Fig.
R2712	T3528	T3527	cc	and,A
R2713	T3529	T3527	conj	B,A
R2714	T3530	T3529	punct	),B
R2715	T3531	T3518	punct	;,increased
R2716	T3532	T3538	advmod	similarly,expressed
R2717	T3533	T3538	punct	",",expressed
R2718	T3534	T3536	compound	granzyme,protein
R2719	T3535	T3536	compound	B,protein
R2720	T3536	T3538	nsubjpass	protein,expressed
R2721	T3537	T3538	auxpass	was,expressed
R2722	T3538	T3538	ROOT	expressed,expressed
R2723	T3539	T3538	prep	by,expressed
R2724	T3540	T3539	pobj	day,by
R2725	T3541	T3540	nummod	4,day
R2726	T3542	T3538	cc	and,expressed
R2727	T3543	T3538	conj	remained,expressed
R2728	T3544	T3543	acomp	high,remained
R2729	T3545	T3543	prep	until,remained
R2730	T3546	T3545	pobj	day,until
R2731	T3547	T3546	nummod	6,day
R2732	T3548	T3549	punct	(,Fig.
R2733	T3549	T3546	appos	Fig.,day
R2734	T3550	T3549	nummod	1,Fig.
R2735	T3551	T3549	npadvmod	E,Fig.
R2736	T3552	T3549	punct	),Fig.
R2737	T3553	T3500	punct	.,was
R2738	T3554	T3555	mark	As,expected
R2739	T3555	T3564	advcl	expected,expressed
R2740	T3556	T3564	punct	",",expressed
R2741	T3557	T3559	det	a,fraction
R2742	T3558	T3559	amod	small,fraction
R2743	T3559	T3564	nsubj	fraction,expressed
R2744	T3560	T3559	prep	of,fraction
R2745	T3561	T3563	amod	naive,cells
R2746	T3562	T3563	compound	T,cells
R2747	T3563	T3560	pobj	cells,of
R2748	T3564	T3564	ROOT	expressed,expressed
R2749	T3565	T3566	det	the,cytokines
R2750	T3566	T3567	compound	cytokines,IFN-γ
R2751	T3567	T3564	dobj	IFN-γ,expressed
R2752	T3568	T3567	cc	and,IFN-γ
R2753	T3569	T3567	conj	TNF,IFN-γ
R2754	T3570	T3564	prep	in,expressed
R2755	T3571	T3570	pobj	response,in
R2756	T3572	T3571	prep	to,response
R2757	T3573	T3572	pobj	stimulation,to
R2758	T3574	T3564	punct	",",expressed
R2759	T3575	T3564	cc	and,expressed
R2760	T3576	T3577	det	this,capacity
R2761	T3577	T3578	nsubj	capacity,increased
R2762	T3578	T3564	conj	increased,expressed
R2763	T3579	T3578	advmod	significantly,increased
R2764	T3580	T3578	prep	in,increased
R2765	T3581	T3582	amod	differentiated,cells
R2766	T3582	T3580	pobj	cells,in
R2767	T3583	T3578	punct	(,increased
R2768	T3584	T3586	nmod	Fig.,E
R2769	T3585	T3586	nummod	1,E
R2770	T3586	T3588	ccomp	E,see
R2771	T3587	T3588	punct	;,see
R2772	T3588	T3578	parataxis	see,increased
R2773	T3589	T3590	advmod	also,Fig.
R2774	T3590	T3592	dative	Fig.,A
R2775	T3591	T3592	nummod	2,A
R2776	T3592	T3588	dobj	A,see
R2777	T3593	T3588	punct	),see
R2778	T3594	T3578	punct	.,increased
R2779	T3595	T3596	nsubj	We,evaluated
R2780	T3596	T3596	ROOT	evaluated,evaluated
R2781	T3597	T3599	amod	antigen-dependent,function
R2782	T3598	T3599	compound	cytolytic,function
R2783	T3599	T3596	dobj	function,evaluated
R2784	T3600	T3596	prep	in,evaluated
R2785	T3601	T3603	det	a,assay
R2786	T3602	T3603	amod	short-term,assay
R2787	T3603	T3600	pobj	assay,in
R2788	T3604	T3610	prep	in,measured
R2789	T3605	T3604	pobj	which,in
R2790	T3606	T3608	compound	target,death
R2791	T3607	T3608	compound	cell,death
R2792	T3608	T3610	nsubjpass	death,measured
R2793	T3609	T3610	auxpass	was,measured
R2794	T3610	T3603	relcl	measured,assay
R2795	T3611	T3610	prep	within,measured
R2796	T3612	T3613	nummod	2,h
R2797	T3613	T3611	pobj	h,within
R2798	T3614	T3617	punct	(,F
R2799	T3615	T3617	nmod	Fig.,F
R2800	T3616	T3615	nummod	1,Fig.
R2801	T3617	T3613	appos	F,h
R2802	T3618	T3613	punct	),h
R2803	T3619	T3596	punct	.,evaluated
R2804	T3620	T3630	prep	By,minimizes
R2805	T3621	T3620	pcomp	limiting,By
R2806	T3622	T3623	det	the,duration
R2807	T3623	T3621	dobj	duration,limiting
R2808	T3624	T3623	prep	of,duration
R2809	T3625	T3626	compound	TCR,stimulation
R2810	T3626	T3624	pobj	stimulation,of
R2811	T3627	T3630	punct	",",minimizes
R2812	T3628	T3629	det	this,strategy
R2813	T3629	T3630	nsubj	strategy,minimizes
R2814	T3630	T3630	ROOT	minimizes,minimizes
R2815	T3631	T3632	amod	cytolysis,secondary
R2816	T3632	T3636	amod	secondary,expression
R2817	T3633	T3636	nmod	to,expression
R2818	T3634	T3636	amod	new,expression
R2819	T3635	T3636	compound	gene,expression
R2820	T3636	T3630	dobj	expression,minimizes
R2821	T3637	T3630	prep	during,minimizes
R2822	T3638	T3639	det	the,period
R2823	T3639	T3637	pobj	period,during
R2824	T3640	T3639	prep	of,period
R2825	T3641	T3642	det	the,assay
R2826	T3642	T3640	pobj	assay,of
R2827	T3643	T3630	punct	.,minimizes
R2828	T3644	T3646	amod	Naive,cells
R2829	T3645	T3646	compound	T,cells
R2830	T3646	T3649	nsubj	cells,display
R2831	T3647	T3649	aux	did,display
R2832	T3648	T3649	neg	not,display
R2833	T3649	T3649	ROOT	display,display
R2834	T3650	T3652	amod	significant,function
R2835	T3651	T3652	amod	cytolytic,function
R2836	T3652	T3649	dobj	function,display
R2837	T3653	T3652	prep	in,function
R2838	T3654	T3656	det	this,assay
R2839	T3655	T3656	amod	short-term,assay
R2840	T3656	T3653	pobj	assay,in
R2841	T3657	T3656	punct	(,assay
R2842	T3658	T3659	amod	unpublished,data
R2843	T3659	T3656	appos	data,assay
R2844	T3660	T3656	punct	),assay
R2845	T3661	T3663	punct	",",likely
R2846	T3662	T3663	advmod	most,likely
R2847	T3663	T3649	advmod	likely,display
R2848	T3664	T3666	mark	because,express
R2849	T3665	T3666	nsubj	they,express
R2850	T3666	T3649	advcl	express,display
R2851	T3667	T3671	amod	immature,forms
R2852	T3668	T3671	punct	(,forms
R2853	T3669	T3671	amod	unprocessed,forms
R2854	T3670	T3671	punct	),forms
R2855	T3671	T3666	dobj	forms,express
R2856	T3672	T3671	prep	of,forms
R2857	T3673	T3672	pobj	perforin,of
R2858	T3674	T3666	cc	and,express
R2859	T3675	T3666	conj	lack,express
R2860	T3676	T3677	det	the,capacity
R2861	T3677	T3675	dobj	capacity,lack
R2862	T3678	T3679	aux	to,degranulate
R2863	T3679	T3677	acl	degranulate,capacity
R2864	T3680	T3683	punct	(,19
R2865	T3681	T3683	nummod	18,19
R2866	T3682	T3683	punct	",",19
R2867	T3683	T3679	dobj	19,degranulate
R2868	T3684	T3679	punct	),degranulate
R2869	T3685	T3649	punct	.,display
R2870	T3686	T3687	advmod	Even,after
R2871	T3687	T3697	prep	after,showed
R2872	T3688	T3687	pobj	activation,after
R2873	T3689	T3688	prep	for,activation
R2874	T3690	T3689	pobj	2,for
R2875	T3691	T3690	cc	or,2
R2876	T3692	T3690	conj	4,2
R2877	T3693	T3697	meta	d,showed
R2878	T3694	T3697	punct	",",showed
R2879	T3695	T3696	det	the,cells
R2880	T3696	T3697	nsubj	cells,showed
R2881	T3697	T3697	ROOT	showed,showed
R2882	T3698	T3700	amod	poor,activity
R2883	T3699	T3700	amod	cytolytic,activity
R2884	T3700	T3697	dobj	activity,showed
R2885	T3701	T3704	punct	(,F
R2886	T3702	T3704	nmod	Fig.,F
R2887	T3703	T3702	nummod	1,Fig.
R2888	T3704	T3700	appos	F,activity
R2889	T3705	T3700	punct	),activity
R2890	T3706	T3697	punct	",",showed
R2891	T3707	T3697	prep	in,showed
R2892	T3708	T3709	amod	striking,contrast
R2893	T3709	T3707	pobj	contrast,in
R2894	T3710	T3709	prep	to,contrast
R2895	T3711	T3712	poss	their,capacity
R2896	T3712	T3710	pobj	capacity,to
R2897	T3713	T3712	prep	for,capacity
R2898	T3714	T3716	amod	efficient,production
R2899	T3715	T3716	compound	cytokine,production
R2900	T3716	T3713	pobj	production,for
R2901	T3717	T3716	punct	(,production
R2902	T3718	T3720	nmod	Fig.,E
R2903	T3719	T3720	nummod	1,E
R2904	T3720	T3716	appos	E,production
R2905	T3721	T3716	punct	),production
R2906	T3722	T3697	punct	.,showed
R2907	T3723	T3724	amod	Only,cells
R2908	T3724	T3725	nsubj	cells,cultured
R2909	T3725	T3725	ROOT	cultured,cultured
R2910	T3726	T3729	mark	until,displayed
R2911	T3727	T3726	pobj	day,until
R2912	T3728	T3727	nummod	6,day
R2913	T3729	T3725	advcl	displayed,cultured
R2914	T3730	T3731	amod	robust,cytotoxicity
R2915	T3731	T3729	dobj	cytotoxicity,displayed
R2916	T3732	T3729	punct	",",displayed
R2917	T3733	T3734	mark	as,judged
R2918	T3734	T3729	advcl	judged,displayed
R2919	T3735	T3734	agent	by,judged
R2920	T3736	T3737	poss	their,ability
R2921	T3737	T3735	pobj	ability,by
R2922	T3738	T3739	aux	to,induce
R2923	T3739	T3737	acl	induce,ability
R2924	T3740	T3739	dobj	apoptosis,induce
R2925	T3741	T3739	prep	in,induce
R2926	T3742	T3744	det	a,number
R2927	T3743	T3744	amod	large,number
R2928	T3744	T3741	pobj	number,in
R2929	T3745	T3744	prep	of,number
R2930	T3746	T3747	compound	target,cells
R2931	T3747	T3745	pobj	cells,of
R2932	T3748	T3744	punct	(,number
R2933	T3749	T3751	nmod	Fig.,F
R2934	T3750	T3749	nummod	1,Fig.
R2935	T3751	T3744	appos	F,number
R2936	T3752	T3744	punct	),number
R2937	T3753	T3725	punct	.,cultured
R2938	T3754	T3755	det	These,results
R2939	T3755	T3756	nsubj	results,show
R2940	T3756	T3756	ROOT	show,show
R2941	T3757	T3782	mark	that,programmed
R2942	T3758	T3782	prep	after,programmed
R2943	T3759	T3762	det	a,stimulus
R2944	T3760	T3762	amod	strong,stimulus
R2945	T3761	T3762	amod	priming,stimulus
R2946	T3762	T3758	pobj	stimulus,after
R2947	T3763	T3762	prep	through,stimulus
R2948	T3764	T3763	pobj	TCRs,through
R2949	T3765	T3764	cc	and,TCRs
R2950	T3766	T3767	amod	co-stimulatory,receptors
R2951	T3767	T3764	conj	receptors,TCRs
R2952	T3768	T3762	prep	in,stimulus
R2953	T3769	T3773	amod	vitro,expression
R2954	T3770	T3773	punct	",",expression
R2955	T3771	T3773	amod	granzyme,expression
R2956	T3772	T3773	compound	B,expression
R2957	T3773	T3768	pobj	expression,in
R2958	T3774	T3773	cc	and,expression
R2959	T3775	T3776	det	the,ability
R2960	T3776	T3773	conj	ability,expression
R2961	T3777	T3778	aux	to,produce
R2962	T3778	T3776	acl	produce,ability
R2963	T3779	T3780	compound	effector,cytokines
R2964	T3780	T3782	nsubjpass	cytokines,programmed
R2965	T3781	T3782	auxpass	are,programmed
R2966	T3782	T3756	ccomp	programmed,show
R2967	T3783	T3782	advmod	early,programmed
R2968	T3784	T3782	punct	",",programmed
R2969	T3785	T3792	mark	whereas,induced
R2970	T3786	T3787	amod	perforin,expression
R2971	T3787	T3792	nsubjpass	expression,induced
R2972	T3788	T3787	cc	and,expression
R2973	T3789	T3790	amod	cytolytic,function
R2974	T3790	T3787	conj	function,expression
R2975	T3791	T3792	auxpass	are,induced
R2976	T3792	T3782	advcl	induced,programmed
R2977	T3793	T3792	advmod	later,induced
R2978	T3794	T3792	punct	",",induced
R2979	T3795	T3792	prep	during,induced
R2980	T3796	T3797	det	the,phase
R2981	T3797	T3795	pobj	phase,during
R2982	T3798	T3797	prep	of,phase
R2983	T3799	T3800	amod	clonal,expansion
R2984	T3800	T3798	pobj	expansion,of
R2985	T3801	T3800	prep	in,expansion
R2986	T3802	T3801	pobj	IL-2,in
R2987	T3803	T3756	punct	.,show
R2988	T3804	T3820	advmod	Therefore,are
R2989	T3805	T3820	punct	",",are
R2990	T3806	T3810	det	the,functions
R2991	T3807	T3810	nummod	two,functions
R2992	T3808	T3810	amod	major,functions
R2993	T3809	T3810	compound	effector,functions
R2994	T3810	T3820	nsubj	functions,are
R2995	T3811	T3810	prep	of,functions
R2996	T3812	T3811	pobj	CTL,of
R2997	T3813	T3812	punct	",",CTL
R2998	T3814	T3815	amod	cytokine,production
R2999	T3815	T3818	nmod	production,activity
R3000	T3816	T3815	cc	and,production
R3001	T3817	T3818	compound	cytolytic,activity
R3002	T3818	T3812	appos	activity,CTL
R3003	T3819	T3820	punct	",",are
R3004	T3820	T3820	ROOT	are,are
R3005	T3821	T3820	neg	not,are
R3006	T3822	T3823	advmod	intrinsically,coregulated
R3007	T3823	T3820	acomp	coregulated,are
R3008	T3824	T3820	punct	.,are
R14236	T18364	T18364	ROOT	Kinetics,Kinetics
R14237	T18365	T18364	prep	of,Kinetics
R14238	T18366	T18367	compound	gene,expression
R14239	T18367	T18365	pobj	expression,of
R14240	T18368	T18364	prep	during,Kinetics
R14241	T18369	T18373	compound	CD8,differentiation
R14242	T18370	T18373	compound	+,differentiation
R14243	T18371	T18372	compound	T,cell
R14244	T18372	T18373	compound	cell,differentiation
R14245	T18373	T18368	pobj	differentiation,during
R14246	T18374	T18364	punct	.,Kinetics
R14247	T18375	T18378	punct	(,Kinetics
R14248	T18376	T18378	det	A,Kinetics
R14249	T18377	T18378	punct	),Kinetics
R14250	T18378	T18392	nmod	Kinetics,expression
R14251	T18379	T18378	prep	of,Kinetics
R14252	T18380	T18379	pobj	Prf1,of
R14253	T18381	T18380	punct	",",Prf1
R14267	T18395	T18396	compound	P14,CD8
R14268	T18396	T18394	dobj	CD8,differentiating
R14269	T18397	T18399	amod	+,cells
R14270	T18398	T18399	compound	T,cells
R14271	T18399	T18394	dobj	cells,differentiating
R14272	T18400	T18399	acl	analyzed,cells
R14273	T18401	T18400	agent	by,analyzed
R14274	T18402	T18403	compound	Northern,blotting
R14275	T18403	T18401	pobj	blotting,by
R14276	T18404	T18392	punct	.,expression
R14277	T18405	T18410	nmod	RNA,cells
R14278	T18406	T18405	prep	from,RNA
R14279	T18407	T18406	pobj	day,from
R14280	T18408	T18410	nummod	7,cells
R14281	T18409	T18410	nummod	Th1,cells
R14282	T18410	T18412	nsubjpass	cells,used
R14283	T18411	T18412	auxpass	was,used
R14284	T18412	T18412	ROOT	used,used
R14285	T18413	T18412	prep	as,used
R14286	T18414	T18415	det	a,control
R14287	T18415	T18413	pobj	control,as
R14288	T18416	T18412	punct	.,used
R14289	T18417	T18422	nsubjpass	Sizes,indicated
R14290	T18418	T18417	prep	of,Sizes
R14291	T18419	T18420	compound	mRNA,transcripts
R14292	T18420	T18418	pobj	transcripts,of
R14293	T18421	T18422	auxpass	are,indicated
R14294	T18422	T18422	ROOT	indicated,indicated
R14295	T18423	T18422	punct	.,indicated
R14296	T18424	T18427	punct	(,Quantification
R14297	T18425	T18427	nmod	B,Quantification
R14298	T18426	T18427	punct	),Quantification
R14299	T18427	T18427	ROOT	Quantification,Quantification
R14300	T18428	T18427	prep	of,Quantification
R14301	T18429	T18431	amod	relative,amounts
R14302	T18430	T18431	compound	mRNA,amounts
R14303	T18431	T18428	pobj	amounts,of
R14304	T18432	T18427	prep	by,Quantification
R14305	T18433	T18434	compound	phosphorimager,analysis
R14306	T18434	T18432	pobj	analysis,by
R14307	T18435	T18427	punct	.,Quantification
R14308	T18436	T18437	punct	(,C
R14309	T18437	T18439	nmod	C,Kinetics
R14310	T18438	T18437	punct	),C
R14311	T18439	T18439	ROOT	Kinetics,Kinetics
R14312	T18440	T18439	prep	of,Kinetics
R14313	T18441	T18442	compound	protein,expression
R14314	T18442	T18440	pobj	expression,of
R14315	T18443	T18439	prep	in,Kinetics
R14316	T18444	T18443	pcomp	differentiating,in
R14317	T18445	T18446	compound	P14,CD8
R14318	T18446	T18444	dobj	CD8,differentiating
R14319	T18447	T18449	amod	+,cells
R14320	T18448	T18449	compound	T,cells
R14321	T18449	T18444	dobj	cells,differentiating
R14338	T18466	T18466	ROOT	amounts,amounts
R14339	T18467	T18466	acl	quantified,amounts
R14340	T18468	T18467	prep	from,quantified
R14341	T18469	T18471	det	the,blots
R14342	T18470	T18471	amod	Western,blots
R14343	T18471	T18468	pobj	blots,from
R14344	T18472	T18466	punct	.,amounts
R14345	T18473	T18476	punct	(,Intracellular
R14346	T18474	T18476	nmod	E,Intracellular
R14347	T18475	T18476	punct	),Intracellular
R14348	T18476	T18476	ROOT	Intracellular,Intracellular
R14349	T18477	T18476	acl	staining,Intracellular
R14350	T18478	T18477	prep	for,staining
R14351	T18479	T18480	compound	granzyme,B
R14352	T18480	T18478	pobj	B,for
R14353	T18481	T18480	punct	",",B
R14354	T18482	T18480	appos	IFN-γ,B
R14355	T18483	T18477	punct	",",staining
R14356	T18484	T18477	cc	and,staining
R14357	T18485	T18477	conj	TNF,staining
R14358	T18486	T18476	punct	.,Intracellular
R14359	T18487	T18488	compound	Granzyme,B
R14360	T18488	T18490	nsubj	B,was
R14361	T18489	T18488	acl	staining,B
R14362	T18490	T18490	ROOT	was,was
R14363	T18491	T18492	amod	specific,relative
R14364	T18492	T18490	acomp	relative,was
R14365	T18493	T18492	prep	to,relative
R14366	T18494	T18496	det	an,control
R14367	T18495	T18496	compound	isotype,control
R14368	T18496	T18493	pobj	control,to
R14369	T18497	T18499	punct	(,depicted
R14370	T18498	T18499	neg	not,depicted
R14371	T18499	T18496	relcl	depicted,control
R14372	T18500	T18490	punct	),was
R14373	T18501	T18490	punct	.,was
R14374	T18502	T18504	nsubjpass	Cells,restimulated
R14375	T18503	T18504	auxpass	were,restimulated
R14376	T18504	T18504	ROOT	restimulated,restimulated
R14377	T18505	T18504	prep	with,restimulated
R14378	T18506	T18505	pobj	PMA,with
R14379	T18507	T18506	cc	and,PMA
R14380	T18508	T18506	conj	ionomycin,PMA
R14381	T18509	T18504	prep	for,restimulated
R14382	T18510	T18511	nummod	4,h.
R14383	T18511	T18509	pobj	h.,for
R14384	T18512	T18513	punct	(,F
R14385	T18513	T18511	appos	F,h.
R14386	T18514	T18504	punct	),restimulated
R14387	T18515	T18516	amod	FACS-based,assay
R14388	T18516	T18524	nsubj	assay,+
R14389	T18517	T18518	aux	to,measure
R14390	T18518	T18516	acl	measure,assay
R14391	T18519	T18520	amod	cytolytic,activity
R14392	T18520	T18518	dobj	activity,measure
R14393	T18521	T18520	prep	of,activity
R14394	T18522	T18523	compound	P14,CD8
R14395	T18523	T18521	pobj	CD8,of
R14396	T18524	T18524	ROOT	+,+
R14397	T18525	T18526	compound	T,cells
R14398	T18526	T18524	dobj	cells,+
R14399	T18527	T18524	prep	against,+
R14400	T18528	T18529	nummod	EL4,targets
R14401	T18529	T18527	pobj	targets,against
R14402	T18530	T18524	prep	loaded,+
R14403	T18531	T18530	prep	with,loaded
R14404	T18532	T18534	nummod	0,−
R14405	T18533	T18532	punct	(,0
R14406	T18534	T18531	pobj	−,with
R14407	T18535	T18530	punct	),loaded
R14408	T18536	T18524	cc	or,+
R14409	T18537	T18539	nummod	1,+
R14410	T18538	T18537	punct	(,1
R14411	T18539	T18543	nmod	+,peptide
R14412	T18540	T18543	punct	),peptide
R14413	T18541	T18543	compound	μM,peptide
R14414	T18542	T18543	compound	Gp33,peptide
R14415	T18543	T18546	nmod	peptide,ratio
R14416	T18544	T18546	punct	(,ratio
R14417	T18545	T18546	amod	effector-to-target,ratio
R14418	T18546	T18548	nsubj	ratio,5:1
R14419	T18547	T18548	punct	=,5:1
R14420	T18548	T18524	conj	5:1,+
R14421	T18549	T18548	punct	),5:1
R14422	T18550	T18524	punct	.,+
R14423	T18551	T18572	nsubjpass	Percentage,determined
R14424	T18552	T18551	prep	of,Percentage
R14425	T18553	T18555	compound	Annexin,+
R14426	T18554	T18555	compound	V,+
R14427	T18555	T18552	pobj	+,of
R14428	T18556	T18557	punct	(,apoptotic
R14429	T18557	T18560	amod	apoptotic,cells
R14430	T18558	T18557	punct	),apoptotic
R14431	T18559	T18560	compound	target,cells
R14432	T18560	T18551	conj	cells,Percentage
R14433	T18561	T18560	prep	in,cells
R14434	T18562	T18566	det	the,population
R14435	T18563	T18566	amod	CD8-negative,population
R14436	T18564	T18566	nummod	EL4,population
R14437	T18565	T18566	compound	target,population
R14438	T18566	T18561	pobj	population,in
R14439	T18567	T18568	punct	(,dot
R14440	T18568	T18569	amod	dot,plots
R14441	T18569	T18560	conj	plots,cells
R14442	T18570	T18560	punct	),cells
R14443	T18571	T18572	auxpass	was,determined
R14444	T18572	T18572	ROOT	determined,determined
R14445	T18573	T18572	punct	(,determined
R14446	T18574	T18572	oprd	histograms,determined
R14447	T18575	T18572	punct	),determined
R14448	T18576	T18572	punct	.,determined
R14449	T18577	T18578	amod	Cytolytic,activity
R14450	T18578	T18580	nsubjpass	activity,blocked
R14451	T18579	T18580	auxpass	was,blocked
R14452	T18580	T18580	ROOT	blocked,blocked
R14453	T18581	T18580	agent	by,blocked
R14454	T18582	T18581	pobj	incubation,by
R14455	T18583	T18580	prep	with,blocked
R14456	T18584	T18586	nummod	2,EGTA
R14457	T18585	T18586	compound	mM,EGTA
R14458	T18586	T18583	pobj	EGTA,with
R14459	T18587	T18589	punct	(,depicted
R14460	T18588	T18589	neg	not,depicted
R14461	T18589	T18586	parataxis	depicted,EGTA
R14462	T18590	T18589	punct	),depicted
R14463	T18591	T18580	punct	",",blocked
R14464	T18592	T18580	advcl	confirming,blocked
R14465	T18593	T18592	dobj	involvement,confirming
R14466	T18594	T18593	prep	of,involvement
R14467	T18595	T18597	det	the,exocytosis
R14468	T18596	T18597	compound	granule,exocytosis
R14469	T18597	T18594	pobj	exocytosis,of
R14470	T18598	T18601	punct	(,B
R14471	T18599	T18601	nmod	perforin,B
R14472	T18600	T18601	compound	granzyme,B
R14473	T18601	T18597	appos	B,exocytosis
R14474	T18602	T18601	punct	),B
R14475	T18603	T18597	appos	pathway,exocytosis
R14476	T18604	T18580	punct	.,blocked
R14477	T18605	T18606	nsubj	Data,are
R14478	T18606	T18606	ROOT	are,are
R14479	T18607	T18622	nmod	representative,experiments
R14480	T18608	T18607	prep	of,representative
R14481	T18609	T18610	advmod	at,least
R14482	T18610	T18611	advmod	least,five
R14483	T18611	T18608	pobj	five,of
R14484	T18612	T18614	punct	(,E
R14485	T18613	T18614	det	A,E
R14486	T18614	T18607	appos	E,representative
R14487	T18615	T18614	punct	),E
R14488	T18616	T18607	cc	or,representative
R14489	T18617	T18619	nummod	three,F
R14490	T18618	T18619	punct	(,F
R14491	T18619	T18607	conj	F,representative
R14492	T18620	T18619	punct	),F
R14493	T18621	T18622	amod	independent,experiments
R14494	T18622	T18606	attr	experiments,are
R14495	T18623	T18606	punct	.,are
R14254	T18382	T18380	conj	Gzmb,Prf1
R14255	T18383	T18382	punct	",",Gzmb
R14256	T18384	T18382	conj	Tbx21,Gzmb
R14257	T18385	T18386	punct	(,T-bet
R14258	T18386	T18384	appos	T-bet,Tbx21
R14259	T18387	T18384	punct	),Tbx21
R14260	T18388	T18384	punct	",",Tbx21
R14261	T18389	T18384	cc	and,Tbx21
R14262	T18390	T18391	compound	Eomes,mRNA
R14263	T18391	T18392	compound	mRNA,expression
R14264	T18392	T18392	ROOT	expression,expression
R14265	T18393	T18392	prep	in,expression
R14266	T18394	T18393	pcomp	differentiating,in
R14322	T18450	T18449	acl	analyzed,cells
R14323	T18451	T18450	agent	by,analyzed
R14324	T18452	T18451	pcomp	immunoblotting,by
R14325	T18453	T18439	punct	.,Kinetics
R14326	T18454	T18459	nsubjpass	Sizes,indicated
R14327	T18455	T18454	prep	of,Sizes
R14328	T18456	T18457	compound	protein,bands
R14329	T18457	T18455	pobj	bands,of
R14330	T18458	T18459	auxpass	are,indicated
R14331	T18459	T18459	ROOT	indicated,indicated
R14332	T18460	T18459	punct	.,indicated
R14333	T18461	T18462	punct	(,D
R14334	T18462	T18462	ROOT	D,D
R14335	T18463	T18462	punct	),D
R14336	T18464	T18466	amod	Relative,amounts
R14337	T18465	T18466	compound	protein,amounts

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T3348	67-78	http://purl.obolibrary.org/obo/GO_0032502	denotes	development
T3349	212-227	http://purl.obolibrary.org/obo/GO_0001775	denotes	cell activation
T3350	851-854	http://purl.obolibrary.org/obo/GO_0043715	denotes	1 E
T3351	1042-1045	http://purl.obolibrary.org/obo/GO_0043715	denotes	1 E
T3352	3049-3052	http://purl.obolibrary.org/obo/GO_0043715	denotes	1 E
T3353	851-854	http://purl.obolibrary.org/obo/GO_0043874	denotes	1 E
T3354	1042-1045	http://purl.obolibrary.org/obo/GO_0043874	denotes	1 E
T3355	3049-3052	http://purl.obolibrary.org/obo/GO_0043874	denotes	1 E
T3356	938-953	http://purl.obolibrary.org/obo/GO_0034612	denotes	TNF in response
T3357	1015-1035	http://purl.obolibrary.org/obo/GO_0030154	denotes	differentiated cells
T3358	2463-2473	http://purl.obolibrary.org/obo/GO_0008219	denotes	cell death
T3359	2468-2473	http://purl.obolibrary.org/obo/GO_0016265	denotes	death
T3360	2538-2541	http://purl.obolibrary.org/obo/GO_0006283	denotes	TCR
T3361	2579-2588	http://purl.obolibrary.org/obo/GO_0019835	denotes	cytolysis
T3362	2606-2621	http://purl.obolibrary.org/obo/GO_0010467	denotes	gene expression
T3363	3023-3042	http://purl.obolibrary.org/obo/GO_0001816	denotes	cytokine production
T3364	3577-3596	http://purl.obolibrary.org/obo/GO_0001816	denotes	cytokine production
T3365	3155-3164	http://purl.obolibrary.org/obo/GO_0006915	denotes	apoptosis
T3366	3155-3164	http://purl.obolibrary.org/obo/GO_0097194	denotes	apoptosis
T18352	1089-1104	http://purl.obolibrary.org/obo/GO_0010467	denotes	gene expression
T18353	1119-1139	http://purl.obolibrary.org/obo/GO_0030154	denotes	cell differentiation
T18354	2249-2259	http://purl.obolibrary.org/obo/GO_0006887	denotes	exocytosis

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T4292	851-854	http://purl.obolibrary.org/obo/GO_0043715	denotes	1 E
T4293	1042-1045	http://purl.obolibrary.org/obo/GO_0043715	denotes	1 E
T4294	3049-3052	http://purl.obolibrary.org/obo/GO_0043715	denotes	1 E
T4295	851-854	http://purl.obolibrary.org/obo/GO_0043874	denotes	1 E
T4296	1042-1045	http://purl.obolibrary.org/obo/GO_0043874	denotes	1 E
T4297	3049-3052	http://purl.obolibrary.org/obo/GO_0043874	denotes	1 E
T4298	3519-3523	http://purl.obolibrary.org/obo/GO_0005134	denotes	IL-2

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T18899	1119-1123	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T18900	1240-1245	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18901	1296-1301	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18902	1503-1508	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18903	1899-1904	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18904	2046-2051	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18905	1643-1656	http://purl.obolibrary.org/obo/GO_0005622	denotes	Intracellular
T4299	212-216	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T4300	2463-2467	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T4301	2662-2667	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T4302	2921-2926	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T4303	3060-3065	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T4304	3193-3198	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T4305	2538-2541	http://purl.obolibrary.org/obo/GO_0042101	denotes	TCR

sentences

Id	Subject	Object	Predicate	Lexical cue
T3367	0-89	Sentence	denotes	Distinct expression kinetics of perforin and granzyme B during CTL development in culture
T3368	90-237	Sentence	denotes	Our experiments revealed clear differences in the kinetics of perforin, granzyme B, and cytokine expression during CD8+ T cell activation (Fig. 1).
T3369	238-340	Sentence	denotes	Naive T cells showed detectable expression of perforin mRNA as well as perforin protein (Fig. 1, A–D).
T3370	341-568	Sentence	denotes	Relative to its expression in naive T cells, perforin (Prf1) mRNA expression did not increase appreciably at day 2 but showed a reproducible decrease at day 4, followed by robust reexpression between days 4 and 8 (Fig. 1, A–D).
T3371	569-856	Sentence	denotes	In contrast, granzyme B (Gzmb) mRNA was low or undetectable in naive T cells but was strongly up-regulated by day 2 after stimulation and increased progressively until day 6 (Fig. 1, A and B); similarly, granzyme B protein was expressed by day 4 and remained high until day 6 (Fig. 1 E).
T3372	857-1066	Sentence	denotes	As expected, a small fraction of naive T cells expressed the cytokines IFN-γ and TNF in response to stimulation, and this capacity increased significantly in differentiated cells (Fig. 1 E; see also Fig. 2 A).
T3373	2375-2509	Sentence	denotes	We evaluated antigen-dependent cytolytic function in a short-term assay in which target cell death was measured within 2 h (Fig. 1 F).
T3374	2510-2653	Sentence	denotes	By limiting the duration of TCR stimulation, this strategy minimizes cytolysis secondary to new gene expression during the period of the assay.
T3375	2654-2880	Sentence	denotes	Naive T cells did not display significant cytolytic function in this short-term assay (unpublished data), most likely because they express immature (unprocessed) forms of perforin and lack the capacity to degranulate (18, 19).
T3376	2881-3054	Sentence	denotes	Even after activation for 2 or 4 d, the cells showed poor cytolytic activity (Fig. 1 F), in striking contrast to their capacity for efficient cytokine production (Fig. 1 E).
T3377	3055-3210	Sentence	denotes	Only cells cultured until day 6 displayed robust cytotoxicity, as judged by their ability to induce apoptosis in a large number of target cells (Fig. 1 F).
T3378	3211-3524	Sentence	denotes	These results show that after a strong priming stimulus through TCRs and co-stimulatory receptors in vitro, granzyme B expression and the ability to produce effector cytokines are programmed early, whereas perforin expression and cytolytic function are induced later, during the phase of clonal expansion in IL-2.
T3379	3525-3655	Sentence	denotes	Therefore, the two major effector functions of CTL, cytokine production and cytolytic activity, are not intrinsically coregulated.
T18355	1077-1276	Sentence	denotes	Kinetics of gene expression during CD8+ T cell differentiation. (A) Kinetics of Prf1, Gzmb, Tbx21 (T-bet), and Eomes mRNA expression in differentiating P14 CD8+ T cells analyzed by Northern blotting.
T18356	1277-1324	Sentence	denotes	RNA from day 7 Th1 cells was used as a control.
T18357	1325-1536	Sentence	denotes	Sizes of mRNA transcripts are indicated. (B) Quantification of relative mRNA amounts by phosphorimager analysis. (C) Kinetics of protein expression in differentiating P14 CD8+ T cells analyzed by immunoblotting.
T18358	1537-1697	Sentence	denotes	Sizes of protein bands are indicated. (D) Relative protein amounts quantified from the Western blots. (E) Intracellular staining for granzyme B, IFN-γ, and TNF.
T18359	1698-1777	Sentence	denotes	Granzyme B staining was specific relative to an isotype control (not depicted).
T18360	1778-2001	Sentence	denotes	Cells were restimulated with PMA and ionomycin for 4 h. (F) FACS-based assay to measure cytolytic activity of P14 CD8+ T cells against EL4 targets loaded with 0 (−) or 1 (+) μM Gp33 peptide (effector-to-target ratio = 5:1).
T18361	2002-2134	Sentence	denotes	Percentage of Annexin V+ (apoptotic) target cells in the CD8-negative EL4 target population (dot plots) was determined (histograms).
T18362	2135-2290	Sentence	denotes	Cytolytic activity was blocked by incubation with 2 mM EGTA (not depicted), confirming involvement of the granule exocytosis (perforin–granzyme B) pathway.
T18363	2291-2375	Sentence	denotes	Data are representative of at least five (A–E) or three (F) independent experiments.
T34	0-89	Sentence	denotes	Distinct expression kinetics of perforin and granzyme B during CTL development in culture
T35	90-237	Sentence	denotes	Our experiments revealed clear differences in the kinetics of perforin, granzyme B, and cytokine expression during CD8+ T cell activation (Fig. 1).
T36	238-340	Sentence	denotes	Naive T cells showed detectable expression of perforin mRNA as well as perforin protein (Fig. 1, A–D).
T37	341-568	Sentence	denotes	Relative to its expression in naive T cells, perforin (Prf1) mRNA expression did not increase appreciably at day 2 but showed a reproducible decrease at day 4, followed by robust reexpression between days 4 and 8 (Fig. 1, A–D).
T38	569-856	Sentence	denotes	In contrast, granzyme B (Gzmb) mRNA was low or undetectable in naive T cells but was strongly up-regulated by day 2 after stimulation and increased progressively until day 6 (Fig. 1, A and B); similarly, granzyme B protein was expressed by day 4 and remained high until day 6 (Fig. 1 E).
T39	857-1066	Sentence	denotes	As expected, a small fraction of naive T cells expressed the cytokines IFN-γ and TNF in response to stimulation, and this capacity increased significantly in differentiated cells (Fig. 1 E; see also Fig. 2 A).
T40	1067-1076	Sentence	denotes	Figure 1.
T41	1077-1276	Sentence	denotes	Kinetics of gene expression during CD8+ T cell differentiation. (A) Kinetics of Prf1, Gzmb, Tbx21 (T-bet), and Eomes mRNA expression in differentiating P14 CD8+ T cells analyzed by Northern blotting.
T42	1277-1324	Sentence	denotes	RNA from day 7 Th1 cells was used as a control.
T43	1325-1536	Sentence	denotes	Sizes of mRNA transcripts are indicated. (B) Quantification of relative mRNA amounts by phosphorimager analysis. (C) Kinetics of protein expression in differentiating P14 CD8+ T cells analyzed by immunoblotting.
T44	1537-1697	Sentence	denotes	Sizes of protein bands are indicated. (D) Relative protein amounts quantified from the Western blots. (E) Intracellular staining for granzyme B, IFN-γ, and TNF.
T45	1698-1777	Sentence	denotes	Granzyme B staining was specific relative to an isotype control (not depicted).
T46	1778-2001	Sentence	denotes	Cells were restimulated with PMA and ionomycin for 4 h. (F) FACS-based assay to measure cytolytic activity of P14 CD8+ T cells against EL4 targets loaded with 0 (−) or 1 (+) μM Gp33 peptide (effector-to-target ratio = 5:1).
T47	2002-2134	Sentence	denotes	Percentage of Annexin V+ (apoptotic) target cells in the CD8-negative EL4 target population (dot plots) was determined (histograms).
T48	2135-2290	Sentence	denotes	Cytolytic activity was blocked by incubation with 2 mM EGTA (not depicted), confirming involvement of the granule exocytosis (perforin–granzyme B) pathway.
T49	2291-2509	Sentence	denotes	Data are representative of at least five (A–E) or three (F) independent experiments.We evaluated antigen-dependent cytolytic function in a short-term assay in which target cell death was measured within 2 h (Fig. 1 F).
T50	2510-2653	Sentence	denotes	By limiting the duration of TCR stimulation, this strategy minimizes cytolysis secondary to new gene expression during the period of the assay.
T51	2654-2880	Sentence	denotes	Naive T cells did not display significant cytolytic function in this short-term assay (unpublished data), most likely because they express immature (unprocessed) forms of perforin and lack the capacity to degranulate (18, 19).
T52	2881-3054	Sentence	denotes	Even after activation for 2 or 4 d, the cells showed poor cytolytic activity (Fig. 1 F), in striking contrast to their capacity for efficient cytokine production (Fig. 1 E).
T53	3055-3210	Sentence	denotes	Only cells cultured until day 6 displayed robust cytotoxicity, as judged by their ability to induce apoptosis in a large number of target cells (Fig. 1 F).
T54	3211-3524	Sentence	denotes	These results show that after a strong priming stimulus through TCRs and co-stimulatory receptors in vitro, granzyme B expression and the ability to produce effector cytokines are programmed early, whereas perforin expression and cytolytic function are induced later, during the phase of clonal expansion in IL-2.
T55	3525-3655	Sentence	denotes	Therefore, the two major effector functions of CTL, cytokine production and cytolytic activity, are not intrinsically coregulated.

events-check-again

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T4491	9-19	Gene_expression	denotes	expression		true
T4492	9-19	Gene_expression	denotes	expression		true
T4493	32-40	Protein	denotes	perforin
T4494	45-55	Protein	denotes	granzyme B
T4495	152-160	Protein	denotes	perforin
T4496	162-172	Protein	denotes	granzyme B
T4497	187-197	Gene_expression	denotes	expression
T4498	187-197	Gene_expression	denotes	expression
T4499	205-208	Protein	denotes	CD8
T4500	270-280	Gene_expression	denotes	expression
T4501	270-280	Gene_expression	denotes	expression
T4502	284-292	Protein	denotes	perforin
T4503	309-317	Protein	denotes	perforin
T4504	357-367	Gene_expression	denotes	expression
T4505	386-394	Protein	denotes	perforin
T4506	396-400	Protein	denotes	Prf1
T4507	402-417	Transcription	denotes	mRNA expression
T4508	426-434	Positive_regulation	denotes	increase	true
T4509	482-490	Negative_regulation	denotes	decrease
T4510	520-532	Positive_regulation	denotes	reexpression
T4511	582-592	Protein	denotes	granzyme B
T4512	594-598	Protein	denotes	Gzmb
T4513	609-628	Transcription	denotes	low or undetectable	true
T4514	663-675	Positive_regulation	denotes	up-regulated
T4515	707-716	Positive_regulation	denotes	increased
T4516	773-783	Protein	denotes	granzyme B
T4517	819-832	Positive_regulation	denotes	remained high
T4518	904-913	Gene_expression	denotes	expressed
T4519	904-913	Gene_expression	denotes	expressed
T4520	928-933	Protein	denotes	IFN-γ
T4521	938-941	Protein	denotes	TNF
T4522	988-997	Positive_regulation	denotes	increased
T4523	988-997	Positive_regulation	denotes	increased
T4524	2785-2792	Gene_expression	denotes	express
T4525	2825-2833	Protein	denotes	perforin
T4526	3319-3329	Protein	denotes	granzyme B
T4527	3330-3340	Gene_expression	denotes	expression
T4528	3417-3425	Protein	denotes	perforin
T4529	3426-3436	Gene_expression	denotes	expression
T4530	3464-3471	Positive_regulation	denotes	induced
T4531	3519-3523	Protein	denotes	IL-2
T19018	1112-1115	Protein	denotes	CD8
T19019	1157-1161	Protein	denotes	Prf1
T19020	1163-1167	Protein	denotes	Gzmb
T19021	1169-1174	Protein	denotes	Tbx21
T19022	1176-1181	Protein	denotes	T-bet
T19023	1188-1193	Protein	denotes	Eomes
T19024	1194-1209	Transcription	denotes	mRNA expression		true
T19025	1194-1209	Transcription	denotes	mRNA expression		true
T19026	1194-1209	Transcription	denotes	mRNA expression		true
T19027	1194-1209	Transcription	denotes	mRNA expression		true
T19028	1233-1236	Protein	denotes	CD8
T19029	1496-1499	Protein	denotes	CD8
T19030	1657-1665	Gene_expression	denotes	staining		true
T19031	1657-1665	Gene_expression	denotes	staining		true
T19032	1657-1665	Gene_expression	denotes	staining		true
T19033	1670-1680	Protein	denotes	granzyme B
T19034	1682-1687	Protein	denotes	IFN-γ
T19035	1693-1696	Protein	denotes	TNF
T19036	1698-1708	Protein	denotes	Granzyme B
T19037	1709-1717	Gene_expression	denotes	staining		true
T19038	1892-1895	Protein	denotes	CD8
T19039	2016-2025	Protein	denotes	Annexin V
T19040	2059-2062	Protein	denotes	CD8
T19041	2261-2269	Protein	denotes	perforin
T19042	2270-2280	Protein	denotes	granzyme B
R3556	T4493	T4492	themeOf	perforin,expression
R3557	T4494	T4491	themeOf	granzyme B,expression
R3558	T4495	T4498	themeOf	perforin,expression
R3559	T4496	T4497	themeOf	granzyme B,expression
R3560	T4502	T4501	themeOf	perforin,expression
R3561	T4503	T4500	themeOf	perforin,expression
R3562	T4505	T4504	themeOf	perforin,expression
R3563	T4505	T4507	themeOf	perforin,mRNA expression
R3564	T4506	T4505	equivalentTo	Prf1,perforin
R3565	T4507	T4508	themeOf	mRNA expression,increase
R3566	T4507	T4509	themeOf	mRNA expression,decrease
R3567	T4507	T4510	themeOf	mRNA expression,reexpression
R3568	T4511	T4513	themeOf	granzyme B,low or undetectable
R3569	T4511	T4514	themeOf	granzyme B,up-regulated
R3570	T4511	T4515	themeOf	granzyme B,increased
R3571	T4512	T4511	equivalentTo	Gzmb,granzyme B
R3572	T4516	T4517	themeOf	granzyme B,remained high
R3573	T4518	T4522	themeOf	expressed,increased
R3574	T4519	T4523	themeOf	expressed,increased
R3575	T4520	T4518	themeOf	IFN-γ,expressed
R3576	T4521	T4519	themeOf	TNF,expressed
R3577	T4525	T4524	themeOf	perforin,express
R3578	T4526	T4527	themeOf	granzyme B,expression
R3579	T4528	T4529	themeOf	perforin,expression
R3580	T4529	T4530	themeOf	expression,induced
R14774	T19019	T19025	themeOf	Prf1,mRNA expression
R14775	T19020	T19027	themeOf	Gzmb,mRNA expression
R14776	T19021	T19026	themeOf	Tbx21,mRNA expression
R14777	T19022	T19021	equivalentTo	T-bet,Tbx21
R14778	T19023	T19024	themeOf	Eomes,mRNA expression
R14779	T19033	T19031	themeOf	granzyme B,staining
R14780	T19034	T19032	themeOf	IFN-γ,staining
R14781	T19035	T19030	themeOf	TNF,staining
R14782	T19036	T19037	themeOf	Granzyme B,staining

bionlp-st-ge-2016-reference-tees

Id	Subject	Object	Predicate	Lexical cue
T4532	32-40	Protein	denotes	perforin
T4533	45-55	Protein	denotes	granzyme B
T4534	9-19	Gene_expression	denotes	expression
T4535	9-19	Gene_expression	denotes	expression
T4536	205-209	Protein	denotes	CD8+
T4537	284-297	Protein	denotes	perforin mRNA
T4538	309-317	Protein	denotes	perforin
T4539	270-280	Gene_expression	denotes	expression
T4540	270-280	Gene_expression	denotes	expression
T4541	386-394	Protein	denotes	perforin
T4542	396-400	Protein	denotes	Prf1
T4543	407-417	Transcription	denotes	expression
T4544	407-417	Transcription	denotes	expression
T4545	426-434	Positive_regulation	denotes	increase
T4546	426-434	Positive_regulation	denotes	increase
T4547	482-490	Negative_regulation	denotes	decrease
T4548	482-490	Negative_regulation	denotes	decrease
T4549	520-532	Positive_regulation	denotes	reexpression
T4550	520-532	Positive_regulation	denotes	reexpression
T4551	582-592	Protein	denotes	granzyme B
T4552	594-598	Protein	denotes	Gzmb
T4553	773-791	Protein	denotes	granzyme B protein
T4554	616-628	Transcription	denotes	undetectable
T4555	616-628	Transcription	denotes	undetectable
T4556	663-675	Positive_regulation	denotes	up-regulated
T4557	663-675	Positive_regulation	denotes	up-regulated
T4558	707-716	Positive_regulation	denotes	increased
T4559	707-716	Positive_regulation	denotes	increased
T4560	796-805	Gene_expression	denotes	expressed
T4561	828-832	Positive_regulation	denotes	high
T4562	928-933	Protein	denotes	IFN-γ
T4563	938-941	Protein	denotes	TNF
T4564	1056-1064	Protein	denotes	Fig. 2 A
T4565	904-913	Gene_expression	denotes	expressed
T4566	904-913	Gene_expression	denotes	expressed
T4567	1047-1050	Gene_expression	denotes	see
T4568	988-997	Positive_regulation	denotes	increased
T4569	2538-2541	Protein	denotes	TCR
T4570	2825-2833	Protein	denotes	perforin
T4571	2785-2792	Gene_expression	denotes	express
T4572	3275-3279	Protein	denotes	TCRs
T4573	3319-3329	Protein	denotes	granzyme B
T4574	3417-3425	Protein	denotes	perforin
T4575	3519-3523	Protein	denotes	IL-2
T4576	3330-3340	Gene_expression	denotes	expression
T4577	3426-3436	Gene_expression	denotes	expression
T4578	3464-3471	Positive_regulation	denotes	induced
T19043	1112-1116	Protein	denotes	CD8+
T19044	1157-1161	Protein	denotes	Prf1
T19045	1163-1167	Protein	denotes	Gzmb
T19046	1169-1174	Protein	denotes	Tbx21
T19047	1188-1198	Protein	denotes	Eomes mRNA
T19048	1229-1232	Protein	denotes	P14
T19049	1199-1209	Gene_expression	denotes	expression
T19050	1199-1209	Gene_expression	denotes	expression
T19051	1199-1209	Gene_expression	denotes	expression
T19052	1199-1209	Gene_expression	denotes	expression
T19053	1492-1495	Protein	denotes	P14
T19054	1670-1680	Protein	denotes	granzyme B
T19055	1682-1687	Protein	denotes	IFN-γ
T19056	1693-1696	Protein	denotes	TNF
T19057	1657-1665	Gene_expression	denotes	staining
T19058	1657-1665	Gene_expression	denotes	staining
T19059	1657-1665	Gene_expression	denotes	staining
T19060	1888-1891	Protein	denotes	P14
T19061	1952-1967	Protein	denotes	μM Gp33 peptide
T19062	2059-2062	Protein	denotes	CD8
R3581	T4567	T4568	themeOf	see,increased
R3582	T4532	T4534	themeOf	perforin,expression
R3583	T4533	T4535	themeOf	granzyme B,expression
R3584	T4537	T4539	themeOf	perforin mRNA,expression
R3585	T4570	T4571	themeOf	perforin,express
R3586	T4538	T4540	themeOf	perforin,expression
R3587	T4541	T4543	themeOf	perforin,expression
R3588	T4542	T4544	themeOf	Prf1,expression
R3589	T4573	T4576	themeOf	granzyme B,expression
R3590	T4543	T4545	themeOf	expression,increase
R3591	T4543	T4547	themeOf	expression,decrease
R3592	T4543	T4549	themeOf	expression,reexpression
R3593	T4544	T4546	themeOf	expression,increase
R3594	T4544	T4548	themeOf	expression,decrease
R3595	T4544	T4550	themeOf	expression,reexpression
R3596	T4574	T4577	themeOf	perforin,expression
R3597	T4551	T4554	themeOf	granzyme B,undetectable
R3598	T4551	T4556	themeOf	granzyme B,up-regulated
R3599	T4551	T4558	themeOf	granzyme B,increased
R3600	T4577	T4578	themeOf	expression,induced
R3601	T4552	T4555	themeOf	Gzmb,undetectable
R3602	T4552	T4557	themeOf	Gzmb,up-regulated
R3603	T4552	T4559	themeOf	Gzmb,increased
R3604	T4553	T4560	themeOf	granzyme B protein,expressed
R3605	T4553	T4561	themeOf	granzyme B protein,high
R3606	T4562	T4565	themeOf	IFN-γ,expressed
R3607	T4563	T4566	themeOf	TNF,expressed
R3608	T4564	T4567	themeOf	Fig. 2 A,see
R14783	T19044	T19049	themeOf	Prf1,expression
R14784	T19045	T19050	themeOf	Gzmb,expression
R14785	T19046	T19051	themeOf	Tbx21,expression
R14786	T19047	T19052	themeOf	Eomes mRNA,expression
R14787	T19054	T19057	themeOf	granzyme B,staining
R14788	T19055	T19058	themeOf	IFN-γ,staining
R14789	T19056	T19059	themeOf	TNF,staining

bionlp-st-ge-2016-reference

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T18327	1112-1115	Protein	denotes	CD8
T18328	1157-1161	Protein	denotes	Prf1
T18329	1163-1167	Protein	denotes	Gzmb
T18330	1169-1174	Protein	denotes	Tbx21
T18331	1176-1181	Protein	denotes	T-bet
T18332	1188-1193	Protein	denotes	Eomes
T18333	1194-1209	Transcription	denotes	mRNA expression		true
T18334	1194-1209	Transcription	denotes	mRNA expression		true
T18335	1194-1209	Transcription	denotes	mRNA expression		true
T18336	1194-1209	Transcription	denotes	mRNA expression		true
T18337	1233-1236	Protein	denotes	CD8
T18338	1496-1499	Protein	denotes	CD8
T18339	1657-1665	Gene_expression	denotes	staining		true
T18340	1657-1665	Gene_expression	denotes	staining		true
T18341	1657-1665	Gene_expression	denotes	staining		true
T18342	1670-1680	Protein	denotes	granzyme B
T18343	1682-1687	Protein	denotes	IFN-γ
T18344	1693-1696	Protein	denotes	TNF
T18345	1698-1708	Protein	denotes	Granzyme B
T18346	1709-1717	Gene_expression	denotes	staining		true
T18347	1892-1895	Protein	denotes	CD8
T18348	2016-2025	Protein	denotes	Annexin V
T18349	2059-2062	Protein	denotes	CD8
T18350	2261-2269	Protein	denotes	perforin
T18351	2270-2280	Protein	denotes	granzyme B
T3307	9-19	Gene_expression	denotes	expression		true
T3308	9-19	Gene_expression	denotes	expression		true
T3309	32-40	Protein	denotes	perforin
T3310	45-55	Protein	denotes	granzyme B
T3311	152-160	Protein	denotes	perforin
T3312	162-172	Protein	denotes	granzyme B
T3313	187-197	Gene_expression	denotes	expression
T3314	187-197	Gene_expression	denotes	expression
T3315	205-208	Protein	denotes	CD8
T3316	270-280	Gene_expression	denotes	expression
T3317	270-280	Gene_expression	denotes	expression
T3318	284-292	Protein	denotes	perforin
T3319	309-317	Protein	denotes	perforin
T3320	357-367	Gene_expression	denotes	expression
T3321	386-394	Protein	denotes	perforin
T3322	396-400	Protein	denotes	Prf1
T3323	402-417	Transcription	denotes	mRNA expression
T3324	426-434	Positive_regulation	denotes	increase	true
T3325	482-490	Negative_regulation	denotes	decrease
T3326	520-532	Positive_regulation	denotes	reexpression
T3327	582-592	Protein	denotes	granzyme B
T3328	594-598	Protein	denotes	Gzmb
T3329	609-628	Transcription	denotes	low or undetectable	true
T3330	663-675	Positive_regulation	denotes	up-regulated
T3331	707-716	Positive_regulation	denotes	increased
T3332	773-783	Protein	denotes	granzyme B
T3333	819-832	Positive_regulation	denotes	remained high
T3334	904-913	Gene_expression	denotes	expressed
T3335	904-913	Gene_expression	denotes	expressed
T3336	928-933	Protein	denotes	IFN-γ
T3337	938-941	Protein	denotes	TNF
T3338	988-997	Positive_regulation	denotes	increased
T3339	988-997	Positive_regulation	denotes	increased
T3340	2785-2792	Gene_expression	denotes	express
T3341	2825-2833	Protein	denotes	perforin
T3342	3319-3329	Protein	denotes	granzyme B
T3343	3330-3340	Gene_expression	denotes	expression
T3344	3417-3425	Protein	denotes	perforin
T3345	3426-3436	Gene_expression	denotes	expression
T3346	3464-3471	Positive_regulation	denotes	induced
T3347	3519-3523	Protein	denotes	IL-2
R14227	T18328	T18334	themeOf	Prf1,mRNA expression
R14228	T18329	T18336	themeOf	Gzmb,mRNA expression
R14229	T18330	T18335	themeOf	Tbx21,mRNA expression
R14230	T18331	T18330	equivalentTo	T-bet,Tbx21
R14231	T18332	T18333	themeOf	Eomes,mRNA expression
R14232	T18342	T18340	themeOf	granzyme B,staining
R14233	T18343	T18341	themeOf	IFN-γ,staining
R14234	T18344	T18339	themeOf	TNF,staining
R14235	T18345	T18346	themeOf	Granzyme B,staining
R2539	T3309	T3308	themeOf	perforin,expression
R2540	T3310	T3307	themeOf	granzyme B,expression
R2541	T3311	T3314	themeOf	perforin,expression
R2542	T3312	T3313	themeOf	granzyme B,expression
R2543	T3318	T3317	themeOf	perforin,expression
R2544	T3319	T3316	themeOf	perforin,expression
R2545	T3321	T3320	themeOf	perforin,expression
R2546	T3321	T3323	themeOf	perforin,mRNA expression
R2547	T3322	T3321	equivalentTo	Prf1,perforin
R2548	T3323	T3324	themeOf	mRNA expression,increase
R2549	T3323	T3325	themeOf	mRNA expression,decrease
R2550	T3323	T3326	themeOf	mRNA expression,reexpression
R2551	T3327	T3329	themeOf	granzyme B,low or undetectable
R2552	T3327	T3330	themeOf	granzyme B,up-regulated
R2553	T3327	T3331	themeOf	granzyme B,increased
R2554	T3328	T3327	equivalentTo	Gzmb,granzyme B
R2555	T3332	T3333	themeOf	granzyme B,remained high
R2556	T3334	T3338	themeOf	expressed,increased
R2557	T3335	T3339	themeOf	expressed,increased
R2558	T3336	T3334	themeOf	IFN-γ,expressed
R2559	T3337	T3335	themeOf	TNF,expressed
R2560	T3341	T3340	themeOf	perforin,express
R2561	T3342	T3343	themeOf	granzyme B,expression
R2562	T3344	T3345	themeOf	perforin,expression
R2563	T3345	T3346	themeOf	expression,induced

bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T4259	32-40	P14222	denotes	perforin
T4260	45-55	P10144	denotes	granzyme B
T4261	152-160	P14222	denotes	perforin
T4262	162-172	P10144	denotes	granzyme B
T4263	205-208	P01732	denotes	CD8
T4264	205-208	P10966	denotes	CD8
T4265	284-292	P14222	denotes	perforin
T4266	309-317	P14222	denotes	perforin
T4267	341-349	Q04864	denotes	Relative
T4268	386-394	P14222	denotes	perforin
T4269	396-400	P14222	denotes	Prf1
T4270	582-592	P10144	denotes	granzyme B
T4271	594-598	P10144	denotes	Gzmb
T4272	773-783	P10144	denotes	granzyme B
T4273	928-933	P01579	denotes	IFN-γ
T4274	938-941	P01375	denotes	TNF
T4275	2825-2833	P14222	denotes	perforin
T4276	3319-3329	P10144	denotes	granzyme B
T4277	3417-3425	P14222	denotes	perforin
T4278	3519-3523	P60568	denotes	IL-2
T18865	1112-1115	P01732	denotes	CD8
T18866	1112-1115	P10966	denotes	CD8
T18867	1157-1161	P14222	denotes	Prf1
T18868	1163-1167	P10144	denotes	Gzmb
T18869	1169-1174	Q9UL17	denotes	Tbx21
T18870	1176-1181	Q9UL17	denotes	T-bet
T18871	1188-1193	O95936	denotes	Eomes
T18872	1233-1236	P10966	denotes	CD8
T18873	1233-1236	P01732	denotes	CD8
T18874	1388-1396	Q04864	denotes	relative
T18875	1496-1499	P10966	denotes	CD8
T18876	1496-1499	P01732	denotes	CD8
T18877	1579-1587	Q04864	denotes	Relative
T18878	1670-1680	P10144	denotes	granzyme B
T18879	1682-1687	P01579	denotes	IFN-γ
T18880	1693-1696	P01375	denotes	TNF
T18881	1698-1708	P10144	denotes	Granzyme B
T18882	1731-1739	Q04864	denotes	relative
T18883	1892-1895	P01732	denotes	CD8
T18884	1892-1895	P10966	denotes	CD8
T18885	2016-2025	P08758	denotes	Annexin V
T18886	2059-2062	P01732	denotes	CD8
T18887	2059-2062	P10966	denotes	CD8
T18888	2261-2269	P14222	denotes	perforin
T18889	2270-2280	P10144	denotes	granzyme B

test2

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T3270	9-19	Gene_expression	denotes	expression		true
T3271	32-40	Protein	denotes	perforin		true
T3272	45-55	Protein	denotes	granzyme B
T3273	152-160	Protein	denotes	perforin
T3274	162-172	Protein	denotes	granzyme B
T3275	187-197	Gene_expression	denotes	expression
T3276	205-208	Protein	denotes	CD8
T3277	270-280	Gene_expression	denotes	expression
T3278	284-292	Protein	denotes	perforin	true
T3279	309-317	Protein	denotes	perforin
T3280	357-367	Gene_expression	denotes	expression
T3281	386-394	Protein	denotes	perforin	true
T3282	396-400	Protein	denotes	Prf1
T3283	402-417	Transcription	denotes	mRNA expression
T3284	426-434	Positive_regulation	denotes	increase
T3285	482-490	Negative_regulation	denotes	decrease
T3286	582-592	Protein	denotes	granzyme B
T3287	594-598	Protein	denotes	Gzmb
T3288	663-675	Positive_regulation	denotes	up-regulated
T3289	707-716	Positive_regulation	denotes	increased
T3290	773-783	Protein	denotes	granzyme B
T3291	796-805	Gene_expression	denotes	expressed
T3292	828-832	Positive_regulation	denotes	high
T3293	904-913	Gene_expression	denotes	expressed
T3294	928-933	Protein	denotes	IFN-γ
T3295	938-941	Protein	denotes	TNF
T3296	942-953	Positive_regulation	denotes	in response
T3297	988-997	Positive_regulation	denotes	increased
T3298	2785-2792	Gene_expression	denotes	express
T3299	2825-2833	Protein	denotes	perforin
T3300	2838-2842	Negative_regulation	denotes	lack
T3301	3319-3329	Protein	denotes	granzyme B
T3302	3330-3340	Gene_expression	denotes	expression
T3303	3417-3425	Protein	denotes	perforin
T3304	3426-3436	Gene_expression	denotes	expression
T3305	3464-3471	Positive_regulation	denotes	induced
T3306	3519-3523	Protein	denotes	IL-2
T18308	1112-1115	Protein	denotes	CD8		true
T18309	1157-1161	Protein	denotes	Prf1		true
T18310	1163-1167	Protein	denotes	Gzmb		true
T18311	1169-1174	Protein	denotes	Tbx21		true
T18312	1176-1181	Protein	denotes	T-bet		true
T18313	1188-1193	Protein	denotes	Eomes		true
T18314	1194-1209	Transcription	denotes	mRNA expression		true
T18315	1233-1236	Protein	denotes	CD8		true
T18316	1496-1499	Protein	denotes	CD8
T18317	1657-1665	Gene_expression	denotes	staining
T18318	1670-1680	Protein	denotes	granzyme B
T18319	1682-1687	Protein	denotes	IFN-γ
T18320	1693-1696	Protein	denotes	TNF
T18321	1698-1708	Protein	denotes	Granzyme B
T18322	1892-1895	Protein	denotes	CD8
T18323	2016-2025	Protein	denotes	Annexin V
T18324	2059-2062	Protein	denotes	CD8
T18325	2261-2269	Protein	denotes	perforin
T18326	2270-2280	Protein	denotes	granzyme B
R2515	T3271	T3270	themeOf	perforin,expression
R2516	T3272	T3270	themeOf	granzyme B,expression
R2517	T3273	T3275	themeOf	perforin,expression
R2518	T3274	T3275	themeOf	granzyme B,expression
R2519	T3278	T3277	themeOf	perforin,expression
R2520	T3279	T3277	themeOf	perforin,expression
R2521	T3281	T3280	themeOf	perforin,expression
R2522	T3281	T3283	themeOf	perforin,mRNA expression
R2523	T3282	T3281	equivalentTo	Prf1,perforin
R2524	T3283	T3285	themeOf	mRNA expression,decrease
R2525	T3283	T3284	themeOf	mRNA expression,increase
R2526	T3286	T3289	themeOf	granzyme B,increased
R2527	T3286	T3288	themeOf	granzyme B,up-regulated
R2528	T3287	T3286	equivalentTo	Gzmb,granzyme B
R2529	T3287	T3288	themeOf	Gzmb,up-regulated
R2530	T3288	T3289	themeOf	up-regulated,increased
R2531	T3290	T3291	themeOf	granzyme B,expressed
R2532	T3293	T3297	themeOf	expressed,increased
R2533	T3294	T3293	themeOf	IFN-γ,expressed
R2534	T3295	T3293	themeOf	TNF,expressed
R2535	T3299	T3298	themeOf	perforin,express
R2536	T3301	T3302	themeOf	granzyme B,expression
R2537	T3303	T3304	themeOf	perforin,expression
R2538	T3304	T3305	themeOf	expression,induced
R14219	T18309	T18314	themeOf	Prf1,mRNA expression
R14220	T18310	T18314	themeOf	Gzmb,mRNA expression
R14221	T18311	T18314	themeOf	Tbx21,mRNA expression
R14222	T18312	T18311	equivalentTo	T-bet,Tbx21
R14223	T18313	T18314	themeOf	Eomes,mRNA expression
R14224	T18318	T18317	themeOf	granzyme B,staining
R14225	T18319	T18317	themeOf	IFN-γ,staining
R14226	T18320	T18317	themeOf	TNF,staining

PMC:2626671 / 5469-9124 JSONTXT

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PMC:2626671 / 5469-9124 JSON TXT