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PMC:2626671 / 33613-35021 JSONTXT

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2_test

Id Subject Object Predicate Lexical cue
19139168-16510851-59674077 116-118 16510851 denotes 29

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T17850 129-130 -COMMA- denotes ,
T17851 131-133 CD denotes 10
T17852 134-136 NN denotes μg
T17853 137-139 IN denotes of
T17854 140-145 JJ denotes total
T17855 146-149 NN denotes RNA
T17856 150-153 VB denotes was
T17857 154-160 VB denotes loaded
T17858 161-164 IN denotes per
T17859 165-169 NN denotes lane
T17860 170-173 CC denotes and
T17861 174-185 VB denotes transferred
T17862 186-188 TO denotes to
T17863 189-199 RB denotes positively
T17864 200-207 VB denotes charged
T17865 208-213 NN denotes nylon
T17866 214-223 NN denotes membranes
T17867 224-225 -LRB- denotes (
T17868 225-234 JJ denotes Hybond-N+
T17869 234-235 -COLON- denotes ;
T17870 236-238 NNP denotes GE
T17871 239-249 NNP denotes Healthcare
T17872 249-250 -RRB- denotes )
T17873 250-251 -COMMA- denotes ,
T17874 252-257 WDT denotes which
T17875 258-261 VB denotes was
T17876 262-271 VB denotes confirmed
T17877 272-274 IN denotes by
T17878 275-283 NN denotes ethidium
T17879 284-291 NN denotes bromide
T17880 292-300 NN denotes staining
T17881 301-303 IN denotes of
T17882 304-313 JJ denotes ribosomal
T17883 314-317 NN denotes RNA
T17884 318-325 NN denotes species
T17885 326-328 IN denotes on
T17886 329-332 DT denotes the
T17887 333-341 NN denotes membrane
T17888 343-352 NN denotes Membranes
T17889 353-357 VB denotes were
T17890 358-368 VB denotes hybridized
T17891 369-373 IN denotes with
T17892 374-375 CD denotes 1
T17893 376-381 NN denotes ng/ml
T17894 382-384 SYM denotes α-
T17895 384-385 -LRB- denotes [
T17896 385-388 NN denotes 32P
T17897 388-389 -RRB- denotes ]
T17898 389-401 VB denotes dCTP–labeled
T17899 402-417 JJ denotes trichloroacetic
T17900 418-422 NN denotes acid
T17901 423-435 JJ denotes precipitable
T17902 436-441 NN denotes probe
T17903 442-444 IN denotes in
T17904 445-455 NN denotes ExpressHyb
T17905 456-469 NN denotes hybridization
T17906 470-476 NN denotes buffer
T17907 477-478 -LRB- denotes (
T17908 478-486 NNP denotes Clontech
T17909 487-499 NNP denotes Laboratories
T17910 499-500 -COMMA- denotes ,
T17911 501-505 NNP denotes Inc.
T17912 505-506 -RRB- denotes )
T17913 508-511 DT denotes All
T17914 512-516 NN denotes cDNA
T17915 517-523 NN denotes probes
T17916 524-528 VB denotes were
T17917 529-538 VB denotes confirmed
T17918 539-541 TO denotes to
T17919 542-546 VB denotes have
T17920 547-550 DT denotes the
T17921 551-562 JJ denotes appropriate
T17922 563-574 JJ denotes single-copy
T17923 575-586 NN denotes specificity
T17924 587-592 IN denotes under
T17925 593-598 DT denotes these
T17926 599-609 NN denotes conditions
T17927 610-615 VB denotes using
T17928 616-623 JJ denotes genomic
T17929 624-632 JJ denotes Southern
T17930 633-637 NN denotes blot
T17931 638-646 NN denotes analysis
T17932 648-652 NN denotes Band
T17933 653-664 NN denotes intensities
T17934 665-669 VB denotes were
T17935 670-678 VB denotes acquired
T17936 679-681 IN denotes by
T17937 682-697 VB denotes phosphorimaging
T17938 698-706 NN denotes analysis
T17939 708-711 IN denotes For
T17940 712-719 JJ denotes Western
T17941 720-728 NN denotes analysis
T17942 728-729 -COMMA- denotes ,
T17943 730-740 JJ denotes whole-cell
T17944 741-748 NN denotes protein
T17945 749-756 NN denotes lysates
T17946 757-761 VB denotes were
T17947 762-770 VB denotes obtained
T17948 771-775 IN denotes from
T17949 776-780 JJ denotes CD8+
T17950 781-782 NN denotes T
T17951 783-788 NN denotes cells
T17952 789-791 IN denotes at
T17953 792-795 DT denotes the
T17954 796-800 NN denotes time
T17955 801-807 NN denotes points
T17956 808-817 VB denotes indicated
T17957 818-820 IN denotes in
T17958 821-824 DT denotes the
T17959 825-832 NN denotes figures
T17960 833-839 IN denotes during
T17961 840-846 JJ denotes clonal
T17962 847-856 NN denotes expansion
T17963 857-859 IN denotes in
T17964 860-863 CD denotes 100
T17965 864-868 NN denotes U/ml
T17966 869-873 NN denotes IL-2
T17967 874-878 IN denotes with
T17968 879-884 NN denotes lysis
T17969 885-891 NN denotes buffer
T17970 892-893 -LRB- denotes (
T17971 893-895 CD denotes 50
T17972 896-898 NN denotes mM
T17973 899-903 NN denotes Tris
T17974 904-905 -LRB- denotes [
T17975 905-907 NN denotes pH
T17976 908-911 CD denotes 7.5
T17977 911-912 -RRB- denotes ]
T17978 912-913 -COMMA- denotes ,
T17979 914-917 CD denotes 150
T17980 918-920 NN denotes mM
T17981 921-925 NN denotes NaCl
T17982 925-926 -COMMA- denotes ,
T17983 927-929 CD denotes 10
T17984 929-930 NN denotes %
T17985 931-939 NN denotes glycerol
T17986 939-940 -COMMA- denotes ,
T17987 941-942 CD denotes 5
T17988 943-945 NN denotes mM
T17989 946-950 NN denotes EDTA
T17990 950-951 -COMMA- denotes ,
T17991 952-953 CD denotes 1
T17992 953-954 NN denotes %
T17993 955-960 NN denotes NP-40
T17994 960-961 -RRB- denotes )
T17995 962-964 IN denotes by
T17996 965-977 VB denotes resuspending
T17997 978-985 NN denotes samples
T17998 986-988 IN denotes in
T17999 989-991 CD denotes 10
T18000 992-994 NN denotes μl
T18001 995-998 IN denotes per
T18002 999-1002 CD denotes 106
T18003 1003-1008 NN denotes cells
T18004 1009-1012 CC denotes and
T18005 1013-1023 VB denotes incubating
T18006 1024-1026 IN denotes on
T18007 1027-1030 NN denotes ice
T18008 1031-1034 IN denotes for
T18009 1035-1037 CD denotes 30
T18010 1038-1041 NN denotes min
T18011 1042-1044 IN denotes in
T18012 1045-1048 DT denotes the
T18013 1049-1057 NN denotes presence
T18014 1058-1060 IN denotes of
T18015 1061-1069 NN denotes protease
T18016 1070-1080 NN denotes inhibitors
T18017 1082-1092 NN denotes Immunoblot
T18018 1093-1101 NN denotes analysis
T18019 1102-1105 VB denotes was
T18020 1106-1115 VB denotes performed
T18021 1116-1120 IN denotes with
T18022 1121-1124 DT denotes the
T18023 1125-1135 NN denotes antibodies
T18024 1136-1145 VB denotes indicated
T18025 1146-1148 IN denotes in
T18026 1149-1152 DT denotes the
T18027 1153-1160 NN denotes figures
T18028 1161-1166 IN denotes after
T18029 1167-1175 NN denotes SDS-PAGE
T18030 1176-1177 -LRB- denotes (
T18031 1177-1182 CD denotes 10–30
T18032 1183-1185 NN denotes μg
T18033 1186-1188 IN denotes of
T18034 1189-1194 JJ denotes total
T18035 1195-1202 NN denotes protein
T18036 1203-1206 VB denotes was
T18037 1207-1213 VB denotes loaded
T18038 1214-1217 IN denotes per
T18039 1218-1222 RB denotes well
T18040 1222-1223 -RRB- denotes )
T18041 1225-1239 NN denotes Quantification
T18042 1240-1242 IN denotes of
T18043 1243-1251 VB denotes detected
T18044 1252-1259 NN denotes protein
T18045 1260-1263 VB denotes was
T18046 1264-1273 VB denotes performed
T18047 1274-1278 IN denotes with
T18048 1279-1281 DT denotes an
T18049 1282-1293 NNP denotes Intelligent
T18050 1294-1298 NNP denotes Dark
T18051 1299-1302 NNP denotes Box
T18052 1303-1307 NN denotes unit
T18053 1308-1309 -LRB- denotes (
T18054 1309-1317 NN denotes LAS-3000
T18055 1317-1318 -COLON- denotes ;
T18056 1319-1327 NNP denotes Fujifilm
T18057 1327-1328 -RRB- denotes )
T18058 1329-1332 CC denotes and
T18059 1333-1343 VB denotes normalized
T18060 1344-1347 IN denotes for
T18061 1348-1355 VB denotes loading
T18062 1356-1360 IN denotes with
T18063 1361-1364 DT denotes the
T18064 1365-1371 NN denotes amount
T18065 1372-1374 IN denotes of
T18066 1375-1378 NN denotes RNA
T18067 1379-1385 NN denotes Pol-II
T18068 1386-1394 VB denotes detected
T18069 1395-1397 IN denotes in
T18070 1398-1402 DT denotes each
T18071 1403-1407 NN denotes lane
T17829 0-8 JJ denotes Northern
T17830 9-12 CC denotes and
T17831 13-20 JJ denotes Western
T17832 21-25 NN denotes blot
T17833 26-34 NN denotes analyses
T17834 36-39 NN denotes RNA
T17835 40-49 NN denotes isolation
T17836 50-53 CC denotes and
T17837 54-62 JJ denotes Northern
T17838 63-67 NN denotes blot
T17839 68-76 NN denotes analysis
T17840 77-80 VB denotes was
T17841 81-90 VB denotes performed
T17842 91-93 IN denotes as
T17843 94-104 RB denotes previously
T17844 105-114 VB denotes described
T17845 115-116 -LRB- denotes (
T17846 116-118 CD denotes 29
T17847 118-119 -RRB- denotes )
T17848 121-123 IN denotes In
T17849 124-129 NN denotes brief
R13874 T17860 T17856 arg2Of and,was
R13875 T17861 T17860 arg2Of transferred,and
R13876 T17861 T17862 arg1Of transferred,to
R13877 T17864 T17863 arg1Of charged,positively
R13878 T17866 T17862 arg2Of membranes,to
R13879 T17866 T17864 arg1Of membranes,charged
R13880 T17866 T17865 arg1Of membranes,nylon
R13881 T17866 T17867 arg1Of membranes,(
R13882 T17866 T17873 arg1Of membranes,","
R13883 T17866 T17874 arg1Of membranes,which
R13884 T17866 T17875 arg1Of membranes,was
R13885 T17866 T17876 arg2Of membranes,confirmed
R13886 T17868 T17869 arg1Of Hybond-N+,;
R13887 T17869 T17867 arg2Of ;,(
R13888 T17871 T17869 arg2Of Healthcare,;
R13889 T17871 T17870 arg1Of Healthcare,GE
R13890 T17872 T17867 arg3Of ),(
R13891 T17876 T17875 arg2Of confirmed,was
R13892 T17880 T17876 arg1Of staining,confirmed
R13893 T17880 T17877 arg2Of staining,by
R13894 T17880 T17878 arg1Of staining,ethidium
R13895 T17880 T17879 arg1Of staining,bromide
R13896 T17880 T17881 arg1Of staining,of
R13897 T17880 T17885 arg1Of staining,on
R13898 T17884 T17881 arg2Of species,of
R13899 T17884 T17882 arg1Of species,ribosomal
R13900 T17884 T17883 arg1Of species,RNA
R13901 T17887 T17885 arg2Of membrane,on
R13902 T17887 T17886 arg1Of membrane,the
R13903 T17888 T17889 arg1Of Membranes,were
R13904 T17888 T17890 arg2Of Membranes,hybridized
R13905 T17890 T17889 arg2Of hybridized,were
R13906 T17890 T17891 arg1Of hybridized,with
R13907 T17892 T17893 arg1Of 1,ng/ml
R13908 T17894 T17895 arg1Of α-,[
R13909 T17896 T17895 arg2Of 32P,[
R13910 T17897 T17895 arg3Of ],[
R13911 T17898 T17894 arg1Of dCTP–labeled,α-
R13912 T17902 T17891 arg2Of probe,with
R13913 T17902 T17892 arg1Of probe,1
R13914 T17902 T17898 arg1Of probe,dCTP–labeled
R13915 T17902 T17899 arg1Of probe,trichloroacetic
R13916 T17902 T17900 arg1Of probe,acid
R13917 T17902 T17901 arg1Of probe,precipitable
R13918 T17902 T17903 arg1Of probe,in
R13919 T17906 T17903 arg2Of buffer,in
R13920 T17906 T17904 arg1Of buffer,ExpressHyb
R13921 T17906 T17905 arg1Of buffer,hybridization
R13922 T17906 T17907 arg1Of buffer,(
R13923 T17909 T17907 arg2Of Laboratories,(
R13924 T17909 T17908 arg1Of Laboratories,Clontech
R13925 T17909 T17910 arg1Of Laboratories,","
R13926 T17911 T17910 arg2Of Inc.,","
R13927 T17912 T17907 arg3Of ),(
R13928 T17915 T17913 arg1Of probes,All
R13929 T17915 T17914 arg1Of probes,cDNA
R13930 T17915 T17916 arg1Of probes,were
R13931 T17915 T17917 arg2Of probes,confirmed
R13932 T17915 T17919 arg1Of probes,have
R13933 T17917 T17916 arg2Of confirmed,were
R13934 T17919 T17917 arg3Of have,confirmed
R13935 T17919 T17918 arg1Of have,to
R13936 T17919 T17924 arg1Of have,under
R13937 T17923 T17919 arg2Of specificity,have
R13938 T17923 T17920 arg1Of specificity,the
R13939 T17923 T17921 arg1Of specificity,appropriate
R13940 T17923 T17922 arg1Of specificity,single-copy
R13941 T17926 T17924 arg2Of conditions,under
R13942 T17926 T17925 arg1Of conditions,these
R13943 T17926 T17927 arg1Of conditions,using
R13944 T17931 T17927 arg2Of analysis,using
R13945 T17931 T17928 arg1Of analysis,genomic
R13946 T17931 T17929 arg1Of analysis,Southern
R13947 T17931 T17930 arg1Of analysis,blot
R13948 T17933 T17932 arg1Of intensities,Band
R13949 T17933 T17934 arg1Of intensities,were
R13950 T17933 T17935 arg2Of intensities,acquired
R13951 T17935 T17934 arg2Of acquired,were
R13952 T17935 T17936 arg1Of acquired,by
R13953 T17937 T17936 arg2Of phosphorimaging,by
R13954 T17938 T17937 arg2Of analysis,phosphorimaging
R13955 T17941 T17939 arg2Of analysis,For
R13956 T17941 T17940 arg1Of analysis,Western
R13957 T17945 T17943 arg1Of lysates,whole-cell
R13958 T17945 T17944 arg1Of lysates,protein
R13959 T17945 T17946 arg1Of lysates,were
R13960 T17945 T17947 arg2Of lysates,obtained
R13961 T17945 T17996 arg1Of lysates,resuspending
R13962 T17945 T18005 arg1Of lysates,incubating
R13963 T17947 T17939 arg1Of obtained,For
R13964 T17947 T17942 arg1Of obtained,","
R13965 T17947 T17946 arg2Of obtained,were
R13966 T17947 T17948 arg1Of obtained,from
R13967 T17947 T17952 arg1Of obtained,at
R13968 T17947 T17960 arg1Of obtained,during
R13969 T17947 T17995 arg1Of obtained,by
R13970 T17951 T17948 arg2Of cells,from
R13971 T17951 T17949 arg1Of cells,CD8+
R13972 T17951 T17950 arg1Of cells,T
R13973 T17954 T17952 arg2Of time,at
R13974 T17954 T17953 arg1Of time,the
R13975 T17955 T17956 arg1Of points,indicated
R13976 T17956 T17954 arg2Of indicated,time
R13977 T17956 T17957 arg1Of indicated,in
R13978 T17959 T17957 arg2Of figures,in
R13979 T17959 T17958 arg1Of figures,the
R13980 T17962 T17960 arg2Of expansion,during
R13981 T17962 T17961 arg1Of expansion,clonal
R13982 T17962 T17963 arg1Of expansion,in
R13983 T17962 T17967 arg1Of expansion,with
R13984 T17964 T17965 arg1Of 100,U/ml
R13985 T17966 T17963 arg2Of IL-2,in
R13986 T17966 T17964 arg1Of IL-2,100
R13987 T17969 T17967 arg2Of buffer,with
R13988 T17969 T17968 arg1Of buffer,lysis
R13989 T17969 T17970 arg1Of buffer,(
R13990 T17971 T17972 arg1Of 50,mM
R13991 T17973 T17971 arg1Of Tris,50
R13992 T17973 T17974 arg1Of Tris,[
R13993 T17973 T17978 arg1Of Tris,","
R13994 T17975 T17974 arg2Of pH,[
R13995 T17975 T17976 arg1Of pH,7.5
R13996 T17977 T17974 arg3Of ],[
R13997 T17978 T17970 arg2Of ",",(
R13998 T17979 T17980 arg1Of 150,mM
R13999 T17981 T17979 arg1Of NaCl,150
R14000 T17981 T17982 arg1Of NaCl,","
R14001 T17982 T17986 arg1Of ",",","
R14002 T17983 T17984 arg1Of 10,%
R14003 T17985 T17982 arg2Of glycerol,","
R14004 T17985 T17983 arg1Of glycerol,10
R14005 T17986 T17990 arg1Of ",",","
R14006 T17987 T17988 arg1Of 5,mM
R14007 T17989 T17986 arg2Of EDTA,","
R14008 T17989 T17987 arg1Of EDTA,5
R14009 T17990 T17978 arg2Of ",",","
R14010 T17991 T17992 arg1Of 1,%
R14011 T17993 T17990 arg2Of NP-40,","
R14012 T17993 T17991 arg1Of NP-40,1
R14013 T17994 T17970 arg3Of ),(
R14014 T17996 T17998 arg1Of resuspending,in
R14015 T17996 T18004 arg1Of resuspending,and
R14016 T17997 T17996 arg2Of samples,resuspending
R14017 T18000 T17998 arg2Of μl,in
R14018 T18000 T17999 arg1Of μl,10
R14019 T18000 T18001 arg1Of μl,per
R14020 T18003 T18001 arg2Of cells,per
R14021 T18003 T18002 arg1Of cells,106
R14022 T18004 T17995 arg2Of and,by
R14023 T18005 T18004 arg2Of incubating,and
R14024 T18005 T18006 arg1Of incubating,on
R14025 T18005 T18008 arg1Of incubating,for
R14026 T18005 T18011 arg1Of incubating,in
R14027 T18007 T18006 arg2Of ice,on
R14028 T18010 T18008 arg2Of min,for
R14029 T18010 T18009 arg1Of min,30
R14030 T18013 T18011 arg2Of presence,in
R14031 T18013 T18012 arg1Of presence,the
R14032 T18013 T18014 arg1Of presence,of
R14033 T18016 T18014 arg2Of inhibitors,of
R14034 T18016 T18015 arg1Of inhibitors,protease
R14035 T18018 T18017 arg1Of analysis,Immunoblot
R14036 T18018 T18019 arg1Of analysis,was
R14037 T18018 T18020 arg2Of analysis,performed
R14038 T18020 T18019 arg2Of performed,was
R14039 T18020 T18021 arg1Of performed,with
R14040 T18023 T18021 arg2Of antibodies,with
R14041 T18023 T18022 arg1Of antibodies,the
R14042 T18023 T18024 arg2Of antibodies,indicated
R14043 T18024 T18025 arg1Of indicated,in
R14044 T18024 T18028 arg1Of indicated,after
R14045 T18027 T18025 arg2Of figures,in
R14046 T18027 T18026 arg1Of figures,the
R14047 T18029 T18028 arg2Of SDS-PAGE,after
R14048 T18029 T18030 arg1Of SDS-PAGE,(
R14049 T18032 T18031 arg1Of μg,10–30
R14050 T18032 T18033 arg1Of μg,of
R14051 T18032 T18036 arg1Of μg,was
R14052 T18032 T18037 arg2Of μg,loaded
R14053 T18035 T18033 arg2Of protein,of
R14054 T18035 T18034 arg1Of protein,total
R14055 T18037 T18030 arg2Of loaded,(
R14056 T18037 T18036 arg2Of loaded,was
R14057 T18037 T18038 arg1Of loaded,per
R14058 T18039 T18038 arg2Of well,per
R14059 T18040 T18030 arg3Of ),(
R14060 T18041 T18042 arg1Of Quantification,of
R14061 T18041 T18045 arg1Of Quantification,was
R14062 T18041 T18046 arg2Of Quantification,performed
R14063 T18041 T18059 arg2Of Quantification,normalized
R14064 T18041 T18061 arg1Of Quantification,loading
R14065 T18044 T18042 arg2Of protein,of
R14066 T18044 T18043 arg2Of protein,detected
R14067 T18046 T18047 arg1Of performed,with
R14068 T18046 T18058 arg1Of performed,and
R14069 T18051 T18049 arg1Of Box,Intelligent
R14070 T18051 T18050 arg1Of Box,Dark
R14071 T18052 T18047 arg2Of unit,with
R14072 T18052 T18048 arg1Of unit,an
R14073 T18052 T18051 arg1Of unit,Box
R14074 T18052 T18053 arg1Of unit,(
R14075 T18054 T18053 arg2Of LAS-3000,(
R14076 T18054 T18055 arg1Of LAS-3000,;
R14077 T18056 T18055 arg2Of Fujifilm,;
R14078 T18057 T18053 arg3Of ),(
R14079 T18058 T18045 arg2Of and,was
R14080 T18059 T18058 arg2Of normalized,and
R14081 T18059 T18060 arg1Of normalized,for
R14082 T18061 T18060 arg2Of loading,for
R14083 T18061 T18062 arg1Of loading,with
R14084 T18064 T18062 arg2Of amount,with
R14085 T18064 T18063 arg1Of amount,the
R14086 T18064 T18065 arg1Of amount,of
R14087 T18067 T18065 arg2Of Pol-II,of
R14088 T18067 T18066 arg1Of Pol-II,RNA
R14089 T18067 T18068 arg2Of Pol-II,detected
R14090 T18068 T18069 arg1Of detected,in
R14091 T18071 T18069 arg2Of lane,in
R14092 T18071 T18070 arg1Of lane,each
R13842 T17829 T17830 arg1Of Northern,and
R13843 T17831 T17830 arg2Of Western,and
R13844 T17833 T17829 arg1Of analyses,Northern
R13845 T17833 T17831 arg1Of analyses,Western
R13846 T17833 T17832 arg1Of analyses,blot
R13847 T17835 T17834 arg1Of isolation,RNA
R13848 T17835 T17844 arg2Of isolation,described
R13849 T17839 T17836 arg1Of analysis,and
R13850 T17839 T17837 arg1Of analysis,Northern
R13851 T17839 T17838 arg1Of analysis,blot
R13852 T17839 T17840 arg1Of analysis,was
R13853 T17839 T17841 arg2Of analysis,performed
R13854 T17841 T17840 arg2Of performed,was
R13855 T17841 T17842 arg1Of performed,as
R13856 T17842 T17843 arg1Of as,previously
R13857 T17844 T17840 modOf described,was
R13858 T17844 T17845 arg1Of described,(
R13859 T17846 T17845 arg2Of 29,(
R13860 T17847 T17845 arg3Of ),(
R13861 T17849 T17848 arg2Of brief,In
R13862 T17852 T17851 arg1Of μg,10
R13863 T17852 T17853 arg1Of μg,of
R13864 T17852 T17856 arg1Of μg,was
R13865 T17852 T17857 arg2Of μg,loaded
R13866 T17852 T17861 arg2Of μg,transferred
R13867 T17855 T17853 arg2Of RNA,of
R13868 T17855 T17854 arg1Of RNA,total
R13869 T17857 T17858 arg1Of loaded,per
R13870 T17857 T17860 arg1Of loaded,and
R13871 T17859 T17858 arg2Of lane,per
R13872 T17860 T17848 arg1Of and,In
R13873 T17860 T17850 arg1Of and,","

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T17610 208-213 NN denotes nylon
T17611 214-223 NNS denotes membranes
T17612 224-225 -LRB- denotes (
T17613 225-233 JJ denotes Hybond-N
T17614 233-234 NN denotes +
T17615 234-235 : denotes ;
T17616 236-238 NNP denotes GE
T17617 239-249 NNP denotes Healthcare
T17618 249-250 -RRB- denotes )
T17619 250-251 , denotes ,
T17620 252-257 WDT denotes which
T17621 258-261 VBD denotes was
T17622 262-271 VBN denotes confirmed
T17623 272-274 IN denotes by
T17624 275-283 NN denotes ethidium
T17625 284-291 NN denotes bromide
T17626 292-300 NN denotes staining
T17627 301-303 IN denotes of
T17628 304-313 JJ denotes ribosomal
T17629 314-317 NNP denotes RNA
T17630 318-325 NNS denotes species
T17631 326-328 IN denotes on
T17632 329-332 DT denotes the
T17633 333-341 NN denotes membrane
T17634 341-342 . denotes .
T17635 343-352 NNS denotes Membranes
T17636 353-357 VBD denotes were
T17637 358-368 VBN denotes hybridized
T17638 369-373 IN denotes with
T17639 374-375 CD denotes 1
T17640 376-381 NN denotes ng/ml
T17641 382-383 SYM denotes α
T17642 383-384 : denotes -
T17643 384-385 NNP denotes [
T17644 385-388 NNP denotes 32P
T17645 388-389 NNP denotes ]
T17646 389-393 NNP denotes dCTP
T17647 394-401 VBN denotes labeled
T17648 402-417 JJ denotes trichloroacetic
T17649 418-422 NN denotes acid
T17650 423-435 JJ denotes precipitable
T17651 436-441 NN denotes probe
T17652 442-444 IN denotes in
T17653 445-455 NNP denotes ExpressHyb
T17654 456-469 NN denotes hybridization
T17655 470-476 NN denotes buffer
T17656 477-478 -LRB- denotes (
T17657 478-486 NNP denotes Clontech
T17658 487-499 NNPS denotes Laboratories
T17659 499-500 , denotes ,
T17660 501-505 NNP denotes Inc.
T17661 505-506 -RRB- denotes )
T17662 506-507 . denotes .
T17663 508-511 DT denotes All
T17664 512-516 NNP denotes cDNA
T17665 517-523 NNS denotes probes
T17666 524-528 VBD denotes were
T17667 529-538 VBN denotes confirmed
T17668 539-541 TO denotes to
T17669 542-546 VB denotes have
T17670 547-550 DT denotes the
T17671 551-562 JJ denotes appropriate
T17672 563-574 JJ denotes single-copy
T17673 575-586 NN denotes specificity
T17674 587-592 IN denotes under
T17675 593-598 DT denotes these
T17676 599-609 NNS denotes conditions
T17677 610-615 VBG denotes using
T17678 616-623 JJ denotes genomic
T17679 624-632 NNP denotes Southern
T17680 633-637 NN denotes blot
T17681 638-646 NN denotes analysis
T17682 646-647 . denotes .
T17683 648-652 NNP denotes Band
T17684 653-664 NNS denotes intensities
T17685 665-669 VBD denotes were
T17686 670-678 VBN denotes acquired
T17687 679-681 IN denotes by
T17688 682-697 VBG denotes phosphorimaging
T17689 698-706 NN denotes analysis
T17690 706-707 . denotes .
T17691 708-711 IN denotes For
T17692 712-719 JJ denotes Western
T17693 720-728 NN denotes analysis
T17694 728-729 , denotes ,
T17695 730-740 JJ denotes whole-cell
T17696 741-748 NN denotes protein
T17697 749-756 NNS denotes lysates
T17698 757-761 VBD denotes were
T17699 762-770 VBN denotes obtained
T17700 771-775 IN denotes from
T17701 776-779 CD denotes CD8
T17702 779-780 CD denotes +
T17703 781-782 NNP denotes T
T17704 783-788 NNS denotes cells
T17705 789-791 IN denotes at
T17706 792-795 DT denotes the
T17707 796-800 NN denotes time
T17708 801-807 NNS denotes points
T17709 808-817 VBD denotes indicated
T17710 818-820 IN denotes in
T17711 821-824 DT denotes the
T17712 825-832 NNS denotes figures
T17713 833-839 IN denotes during
T17714 840-846 JJ denotes clonal
T17715 847-856 NN denotes expansion
T17716 857-859 IN denotes in
T17717 860-863 CD denotes 100
T17718 864-868 NNP denotes U/ml
T17719 869-873 NN denotes IL-2
T17720 874-878 IN denotes with
T17721 879-884 NN denotes lysis
T17722 885-891 NN denotes buffer
T17723 892-893 -LRB- denotes (
T17724 893-895 CD denotes 50
T17725 896-898 NNP denotes mM
T17726 899-903 NNP denotes Tris
T17727 904-905 NNP denotes [
T17728 905-907 NNP denotes pH
T17729 908-911 CD denotes 7.5
T17730 911-912 NNP denotes ]
T17731 912-913 , denotes ,
T17732 914-917 CD denotes 150
T17733 918-920 NNP denotes mM
T17734 921-925 NNP denotes NaCl
T17735 925-926 , denotes ,
T17736 927-929 CD denotes 10
T17737 929-930 NN denotes %
T17738 931-939 NN denotes glycerol
T17739 939-940 , denotes ,
T17740 941-942 CD denotes 5
T17741 943-945 NNP denotes mM
T17742 946-950 NNP denotes EDTA
T17743 950-951 , denotes ,
T17744 952-953 CD denotes 1
T17745 953-954 NN denotes %
T17746 955-960 NN denotes NP-40
T17747 960-961 -RRB- denotes )
T17748 962-964 IN denotes by
T17749 965-977 VBG denotes resuspending
T17750 978-985 NNS denotes samples
T17751 986-988 IN denotes in
T17752 989-991 CD denotes 10
T17753 992-994 NN denotes μl
T17754 995-998 IN denotes per
T17755 999-1002 CD denotes 106
T17756 1003-1008 NNS denotes cells
T17757 1009-1012 CC denotes and
T17758 1013-1023 VBG denotes incubating
T17759 1024-1026 IN denotes on
T17760 1027-1030 NN denotes ice
T17761 1031-1034 IN denotes for
T17762 1035-1037 CD denotes 30
T17763 1038-1041 NN denotes min
T17764 1042-1044 IN denotes in
T17765 1045-1048 DT denotes the
T17766 1049-1057 NN denotes presence
T17767 1058-1060 IN denotes of
T17768 1061-1069 NN denotes protease
T17769 1070-1080 NNS denotes inhibitors
T17770 1080-1081 . denotes .
T17771 1082-1092 NNP denotes Immunoblot
T17772 1093-1101 NN denotes analysis
T17773 1102-1105 VBD denotes was
T17774 1106-1115 VBN denotes performed
T17775 1116-1120 IN denotes with
T17776 1121-1124 DT denotes the
T17777 1125-1135 NNS denotes antibodies
T17778 1136-1145 VBD denotes indicated
T17779 1146-1148 IN denotes in
T17780 1149-1152 DT denotes the
T17781 1153-1160 NNS denotes figures
T17782 1161-1166 IN denotes after
T17783 1167-1175 NNP denotes SDS-PAGE
T17784 1176-1177 -LRB- denotes (
T17785 1177-1179 CD denotes 10
T17786 1180-1182 CD denotes 30
T17787 1183-1185 NN denotes μg
T17788 1186-1188 IN denotes of
T17789 1189-1194 JJ denotes total
T17790 1195-1202 NN denotes protein
T17791 1203-1206 VBD denotes was
T17792 1207-1213 VBN denotes loaded
T17793 1214-1217 IN denotes per
T17794 1218-1222 NN denotes well
T17795 1222-1223 -RRB- denotes )
T17796 1223-1224 . denotes .
T17797 1225-1239 NN denotes Quantification
T17798 1240-1242 IN denotes of
T17799 1243-1251 VBN denotes detected
T17800 1252-1259 NN denotes protein
T17801 1260-1263 VBD denotes was
T17802 1264-1273 VBN denotes performed
T17803 1274-1278 IN denotes with
T17804 1279-1281 DT denotes an
T17805 1282-1293 NNP denotes Intelligent
T17806 1294-1298 JJ denotes Dark
T17807 1299-1302 NNP denotes Box
T17808 1303-1307 NN denotes unit
T17809 1308-1309 -LRB- denotes (
T17810 1309-1317 NN denotes LAS-3000
T17811 1317-1318 : denotes ;
T17812 1319-1327 NNP denotes Fujifilm
T17813 1327-1328 -RRB- denotes )
T17814 1329-1332 CC denotes and
T17815 1333-1343 VBN denotes normalized
T17816 1344-1347 IN denotes for
T17817 1348-1355 VBG denotes loading
T17818 1356-1360 IN denotes with
T17819 1361-1364 DT denotes the
T17820 1365-1371 NN denotes amount
T17821 1372-1374 IN denotes of
T17822 1375-1378 NNP denotes RNA
T17823 1379-1385 NNP denotes Pol-II
T17824 1386-1394 VBD denotes detected
T17825 1395-1397 IN denotes in
T17826 1398-1402 DT denotes each
T17827 1403-1407 NN denotes lane
T17828 1407-1408 . denotes .
T17572 0-8 NNP denotes Northern
T17573 9-12 CC denotes and
T17574 13-20 NNP denotes Western
T17575 21-25 NN denotes blot
T17576 26-34 NNS denotes analyses
T17577 34-35 . denotes .
T17578 36-39 NNP denotes RNA
T17579 40-49 NN denotes isolation
T17580 50-53 CC denotes and
T17581 54-62 JJ denotes Northern
T17582 63-67 NN denotes blot
T17583 68-76 NN denotes analysis
T17584 77-80 VBD denotes was
T17585 81-90 VBN denotes performed
T17586 91-93 IN denotes as
T17587 94-104 RB denotes previously
T17588 105-114 VBN denotes described
T17589 115-116 -LRB- denotes (
T17590 116-118 CD denotes 29
T17591 118-119 -RRB- denotes )
T17592 119-120 . denotes .
T17593 121-123 IN denotes In
T17594 124-129 NN denotes brief
T17595 129-130 , denotes ,
T17596 131-133 CD denotes 10
T17597 134-136 NN denotes μg
T17598 137-139 IN denotes of
T17599 140-145 JJ denotes total
T17600 146-149 NNP denotes RNA
T17601 150-153 VBD denotes was
T17602 154-160 VBN denotes loaded
T17603 161-164 IN denotes per
T17604 165-169 NN denotes lane
T17605 170-173 CC denotes and
T17606 174-185 VBN denotes transferred
T17607 186-188 TO denotes to
T17608 189-199 RB denotes positively
T17609 200-207 VBN denotes charged
R13635 T17622 T17611 relcl confirmed,membranes
R13636 T17623 T17622 agent by,confirmed
R13637 T17624 T17626 amod ethidium,staining
R13638 T17625 T17626 compound bromide,staining
R13639 T17626 T17623 pobj staining,by
R13640 T17627 T17626 prep of,staining
R13641 T17628 T17630 amod ribosomal,species
R13642 T17629 T17630 compound RNA,species
R13643 T17630 T17627 pobj species,of
R13644 T17631 T17626 prep on,staining
R13645 T17632 T17633 det the,membrane
R13646 T17633 T17631 pobj membrane,on
R13647 T17634 T17602 punct .,loaded
R13648 T17635 T17637 nsubjpass Membranes,hybridized
R13649 T17636 T17637 auxpass were,hybridized
R13650 T17637 T17646 ccomp hybridized,dCTP
R13651 T17638 T17637 prep with,hybridized
R13652 T17639 T17640 nummod 1,ng/ml
R13653 T17640 T17638 pobj ng/ml,with
R13654 T17641 T17637 punct α,hybridized
R13655 T17642 T17646 punct -,dCTP
R13656 T17643 T17646 compound [,dCTP
R13657 T17644 T17646 compound 32P,dCTP
R13658 T17645 T17646 compound ],dCTP
R13659 T17646 T17646 ROOT dCTP,dCTP
R13660 T17647 T17646 acl labeled,dCTP
R13661 T17648 T17649 amod trichloroacetic,acid
R13662 T17649 T17651 nmod acid,probe
R13663 T17650 T17651 amod precipitable,probe
R13664 T17651 T17647 dobj probe,labeled
R13665 T17652 T17647 prep in,labeled
R13666 T17653 T17655 nmod ExpressHyb,buffer
R13667 T17654 T17655 compound hybridization,buffer
R13668 T17655 T17652 pobj buffer,in
R13669 T17656 T17658 punct (,Laboratories
R13670 T17657 T17658 compound Clontech,Laboratories
R13671 T17658 T17655 appos Laboratories,buffer
R13672 T17659 T17658 punct ",",Laboratories
R13673 T17660 T17658 appos Inc.,Laboratories
R13674 T17661 T17658 punct ),Laboratories
R13675 T17662 T17646 punct .,dCTP
R13676 T17663 T17665 det All,probes
R13677 T17664 T17665 compound cDNA,probes
R13678 T17665 T17667 nsubjpass probes,confirmed
R13679 T17666 T17667 auxpass were,confirmed
R13680 T17667 T17667 ROOT confirmed,confirmed
R13681 T17668 T17669 aux to,have
R13682 T17669 T17667 xcomp have,confirmed
R13683 T17670 T17673 det the,specificity
R13684 T17671 T17673 amod appropriate,specificity
R13685 T17672 T17673 amod single-copy,specificity
R13686 T17673 T17669 dobj specificity,have
R13687 T17674 T17669 prep under,have
R13688 T17675 T17676 det these,conditions
R13689 T17676 T17674 pobj conditions,under
R13690 T17677 T17669 advcl using,have
R13691 T17678 T17681 amod genomic,analysis
R13692 T17679 T17680 compound Southern,blot
R13693 T17680 T17681 compound blot,analysis
R13694 T17681 T17677 dobj analysis,using
R13695 T17682 T17667 punct .,confirmed
R13696 T17683 T17684 compound Band,intensities
R13697 T17684 T17686 nsubjpass intensities,acquired
R13698 T17685 T17686 auxpass were,acquired
R13699 T17686 T17686 ROOT acquired,acquired
R13700 T17687 T17686 agent by,acquired
R13701 T17688 T17689 amod phosphorimaging,analysis
R13702 T17689 T17687 pobj analysis,by
R13703 T17690 T17686 punct .,acquired
R13704 T17691 T17699 prep For,obtained
R13705 T17692 T17693 amod Western,analysis
R13706 T17693 T17691 pobj analysis,For
R13707 T17694 T17699 punct ",",obtained
R13708 T17695 T17697 amod whole-cell,lysates
R13709 T17696 T17697 compound protein,lysates
R13710 T17697 T17699 nsubjpass lysates,obtained
R13711 T17698 T17699 auxpass were,obtained
R13712 T17699 T17699 ROOT obtained,obtained
R13732 T17719 T17716 pobj IL-2,in
R13733 T17720 T17719 prep with,IL-2
R13749 T17736 T17737 nummod 10,%
R13750 T17737 T17738 compound %,glycerol
R13751 T17738 T17734 appos glycerol,NaCl
R13752 T17739 T17734 punct ",",NaCl
R13753 T17740 T17742 nummod 5,EDTA
R13754 T17741 T17742 compound mM,EDTA
R13755 T17742 T17734 appos EDTA,NaCl
R13756 T17743 T17742 punct ",",EDTA
R13757 T17744 T17745 nummod 1,%
R13758 T17745 T17746 compound %,NP-40
R13759 T17746 T17742 appos NP-40,EDTA
R13760 T17747 T17742 punct ),EDTA
R13761 T17748 T17709 prep by,indicated
R13783 T17770 T17699 punct .,obtained
R13784 T17771 T17772 compound Immunoblot,analysis
R13785 T17772 T17774 nsubjpass analysis,performed
R13786 T17773 T17774 auxpass was,performed
R13787 T17774 T17774 ROOT performed,performed
R13788 T17775 T17774 prep with,performed
R13789 T17776 T17777 det the,antibodies
R13790 T17777 T17775 pobj antibodies,with
R13791 T17778 T17774 conj indicated,performed
R13792 T17779 T17778 prep in,indicated
R13793 T17780 T17781 det the,figures
R13794 T17781 T17779 pobj figures,in
R13795 T17782 T17792 mark after,loaded
R13796 T17783 T17782 pobj SDS-PAGE,after
R13797 T17784 T17783 punct (,SDS-PAGE
R13798 T17785 T17787 nummod 10,μg
R13799 T17786 T17787 nummod 30,μg
R13800 T17787 T17783 appos μg,SDS-PAGE
R13801 T17788 T17787 prep of,μg
R13802 T17789 T17790 amod total,protein
R13803 T17790 T17788 pobj protein,of
R13804 T17791 T17792 auxpass was,loaded
R13805 T17792 T17778 advcl loaded,indicated
R13806 T17793 T17792 prep per,loaded
R13807 T17794 T17793 pobj well,per
R13808 T17795 T17778 punct ),indicated
R13809 T17796 T17774 punct .,performed
R13810 T17797 T17802 nsubjpass Quantification,performed
R13811 T17798 T17797 prep of,Quantification
R13812 T17799 T17800 amod detected,protein
R13813 T17800 T17798 pobj protein,of
R13814 T17801 T17802 auxpass was,performed
R13815 T17802 T17802 ROOT performed,performed
R13816 T17803 T17802 prep with,performed
R13817 T17804 T17808 det an,unit
R13818 T17805 T17807 compound Intelligent,Box
R13819 T17806 T17807 compound Dark,Box
R13820 T17807 T17808 compound Box,unit
R13821 T17808 T17803 pobj unit,with
R13822 T17809 T17808 punct (,unit
R13823 T17810 T17808 appos LAS-3000,unit
R13824 T17811 T17812 punct ;,Fujifilm
R13825 T17812 T17802 npadvmod Fujifilm,performed
R13826 T17813 T17812 punct ),Fujifilm
R13827 T17814 T17802 cc and,performed
R13828 T17815 T17802 conj normalized,performed
R13829 T17816 T17815 prep for,normalized
R13830 T17817 T17816 pcomp loading,for
R13831 T17818 T17817 prep with,loading
R13832 T17819 T17820 det the,amount
R13833 T17820 T17818 pobj amount,with
R13834 T17821 T17820 prep of,amount
R13835 T17822 T17823 compound RNA,Pol-II
R13836 T17823 T17821 pobj Pol-II,of
R13837 T17824 T17820 acl detected,amount
R13838 T17825 T17824 prep in,detected
R13839 T17826 T17827 det each,lane
R13840 T17827 T17825 pobj lane,in
R13841 T17828 T17802 punct .,performed
R13713 T17700 T17699 prep from,obtained
R13715 T17702 T17704 nummod +,cells
R13716 T17703 T17704 compound T,cells
R13717 T17704 T17700 pobj cells,from
R13718 T17705 T17699 prep at,obtained
R13719 T17706 T17707 det the,time
R13720 T17707 T17705 pobj time,at
R13721 T17708 T17709 nsubj points,indicated
R13722 T17709 T17707 relcl indicated,time
R13723 T17710 T17709 prep in,indicated
R13724 T17711 T17712 det the,figures
R13725 T17712 T17710 pobj figures,in
R13726 T17713 T17709 prep during,indicated
R13727 T17714 T17715 amod clonal,expansion
R13728 T17715 T17713 pobj expansion,during
R13729 T17716 T17715 prep in,expansion
R13730 T17717 T17719 nummod 100,IL-2
R13731 T17718 T17719 compound U/ml,IL-2
R13734 T17721 T17722 compound lysis,buffer
R13735 T17722 T17720 pobj buffer,with
R13736 T17723 T17730 punct (,]
R13737 T17724 T17730 nummod 50,]
R13738 T17725 T17728 compound mM,pH
R13739 T17726 T17728 compound Tris,pH
R13740 T17727 T17728 compound [,pH
R13741 T17728 T17730 compound pH,]
R13742 T17729 T17730 compound 7.5,]
R13743 T17730 T17722 appos ],buffer
R13744 T17731 T17730 punct ",",]
R13745 T17732 T17734 nummod 150,NaCl
R13746 T17733 T17734 compound mM,NaCl
R13747 T17734 T17730 appos NaCl,]
R13748 T17735 T17734 punct ",",NaCl
R13762 T17749 T17748 pcomp resuspending,by
R13763 T17750 T17749 dobj samples,resuspending
R13764 T17751 T17749 prep in,resuspending
R13765 T17752 T17753 nummod 10,μl
R13766 T17753 T17751 pobj μl,in
R13767 T17754 T17753 prep per,μl
R13768 T17755 T17756 nummod 106,cells
R13769 T17756 T17754 pobj cells,per
R13770 T17757 T17749 cc and,resuspending
R13771 T17758 T17749 conj incubating,resuspending
R13772 T17759 T17758 prep on,incubating
R13773 T17760 T17759 pobj ice,on
R13774 T17761 T17758 prep for,incubating
R13775 T17762 T17763 nummod 30,min
R13776 T17763 T17761 pobj min,for
R13777 T17764 T17763 prep in,min
R13778 T17765 T17766 det the,presence
R13779 T17766 T17764 pobj presence,in
R13780 T17767 T17766 prep of,presence
R13781 T17768 T17769 compound protease,inhibitors
R13782 T17769 T17767 pobj inhibitors,of
R13585 T17572 T17576 nmod Northern,analyses
R13586 T17573 T17572 cc and,Northern
R13587 T17574 T17572 conj Western,Northern
R13588 T17575 T17576 compound blot,analyses
R13589 T17576 T17576 ROOT analyses,analyses
R13590 T17577 T17576 punct .,analyses
R13591 T17578 T17579 compound RNA,isolation
R13592 T17579 T17585 nsubjpass isolation,performed
R13593 T17580 T17579 cc and,isolation
R13594 T17581 T17582 amod Northern,blot
R13595 T17582 T17583 compound blot,analysis
R13596 T17583 T17579 conj analysis,isolation
R13597 T17584 T17585 auxpass was,performed
R13598 T17585 T17585 ROOT performed,performed
R13599 T17586 T17588 mark as,described
R13600 T17587 T17588 advmod previously,described
R13601 T17588 T17585 advcl described,performed
R13602 T17589 T17588 punct (,described
R13603 T17590 T17588 npadvmod 29,described
R13604 T17591 T17588 punct ),described
R13605 T17592 T17585 punct .,performed
R13606 T17593 T17602 prep In,loaded
R13607 T17594 T17593 pobj brief,In
R13608 T17595 T17602 punct ",",loaded
R13609 T17596 T17597 nummod 10,μg
R13610 T17597 T17602 nsubjpass μg,loaded
R13611 T17598 T17597 prep of,μg
R13612 T17599 T17600 amod total,RNA
R13613 T17600 T17598 pobj RNA,of
R13614 T17601 T17602 auxpass was,loaded
R13615 T17602 T17602 ROOT loaded,loaded
R13616 T17603 T17602 prep per,loaded
R13617 T17604 T17603 pobj lane,per
R13618 T17605 T17602 cc and,loaded
R13619 T17606 T17602 conj transferred,loaded
R13620 T17607 T17609 aux to,charged
R13621 T17608 T17609 advmod positively,charged
R13622 T17609 T17606 xcomp charged,transferred
R13623 T17610 T17611 compound nylon,membranes
R13624 T17611 T17609 dobj membranes,charged
R13625 T17612 T17611 punct (,membranes
R13626 T17613 T17614 amod Hybond-N,+
R13627 T17614 T17611 appos +,membranes
R13628 T17615 T17611 punct ;,membranes
R13629 T17616 T17617 compound GE,Healthcare
R13630 T17617 T17611 appos Healthcare,membranes
R13631 T17618 T17611 punct ),membranes
R13632 T17619 T17611 punct ",",membranes
R13633 T17620 T17622 nsubjpass which,confirmed
R13634 T17621 T17622 auxpass was,confirmed
R13714 T17701 T17704 nummod CD8,cells

GO-BP

Id Subject Object Predicate Lexical cue
T17562 879-884 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis

GO-MF

Id Subject Object Predicate Lexical cue
T18085 304-317 http://purl.obolibrary.org/obo/GO_0003735 denotes ribosomal RNA
T18086 869-873 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T18087 1125-1135 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies

GO-CC

Id Subject Object Predicate Lexical cue
T18088 214-223 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T18089 333-341 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T18090 343-352 http://purl.obolibrary.org/obo/GO_0016020 denotes Membranes
T18091 304-317 http://purl.obolibrary.org/obo/GO_0005840 denotes ribosomal RNA
T18092 736-740 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T18093 783-788 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18094 1003-1008 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18095 1125-1135 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T18096 1125-1135 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies

sentences

Id Subject Object Predicate Lexical cue
T17563 0-35 Sentence denotes Northern and Western blot analyses.
T17564 36-120 Sentence denotes RNA isolation and Northern blot analysis was performed as previously described (29).
T17565 121-342 Sentence denotes In brief, 10 μg of total RNA was loaded per lane and transferred to positively charged nylon membranes (Hybond-N+; GE Healthcare), which was confirmed by ethidium bromide staining of ribosomal RNA species on the membrane.
T17566 343-507 Sentence denotes Membranes were hybridized with 1 ng/ml α-[32P]dCTP–labeled trichloroacetic acid precipitable probe in ExpressHyb hybridization buffer (Clontech Laboratories, Inc.).
T17567 508-647 Sentence denotes All cDNA probes were confirmed to have the appropriate single-copy specificity under these conditions using genomic Southern blot analysis.
T17568 648-707 Sentence denotes Band intensities were acquired by phosphorimaging analysis.
T17569 708-1081 Sentence denotes For Western analysis, whole-cell protein lysates were obtained from CD8+ T cells at the time points indicated in the figures during clonal expansion in 100 U/ml IL-2 with lysis buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 10% glycerol, 5 mM EDTA, 1% NP-40) by resuspending samples in 10 μl per 106 cells and incubating on ice for 30 min in the presence of protease inhibitors.
T17570 1082-1224 Sentence denotes Immunoblot analysis was performed with the antibodies indicated in the figures after SDS-PAGE (10–30 μg of total protein was loaded per well).
T17571 1225-1408 Sentence denotes Quantification of detected protein was performed with an Intelligent Dark Box unit (LAS-3000; Fujifilm) and normalized for loading with the amount of RNA Pol-II detected in each lane.
T203 0-35 Sentence denotes Northern and Western blot analyses.
T204 36-120 Sentence denotes RNA isolation and Northern blot analysis was performed as previously described (29).
T205 121-342 Sentence denotes In brief, 10 μg of total RNA was loaded per lane and transferred to positively charged nylon membranes (Hybond-N+; GE Healthcare), which was confirmed by ethidium bromide staining of ribosomal RNA species on the membrane.
T206 343-507 Sentence denotes Membranes were hybridized with 1 ng/ml α-[32P]dCTP–labeled trichloroacetic acid precipitable probe in ExpressHyb hybridization buffer (Clontech Laboratories, Inc.).
T207 508-647 Sentence denotes All cDNA probes were confirmed to have the appropriate single-copy specificity under these conditions using genomic Southern blot analysis.
T208 648-707 Sentence denotes Band intensities were acquired by phosphorimaging analysis.
T209 708-1081 Sentence denotes For Western analysis, whole-cell protein lysates were obtained from CD8+ T cells at the time points indicated in the figures during clonal expansion in 100 U/ml IL-2 with lysis buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 10% glycerol, 5 mM EDTA, 1% NP-40) by resuspending samples in 10 μl per 106 cells and incubating on ice for 30 min in the presence of protease inhibitors.
T210 1082-1224 Sentence denotes Immunoblot analysis was performed with the antibodies indicated in the figures after SDS-PAGE (10–30 μg of total protein was loaded per well).
T211 1225-1408 Sentence denotes Quantification of detected protein was performed with an Intelligent Dark Box unit (LAS-3000; Fujifilm) and normalized for loading with the amount of RNA Pol-II detected in each lane.

events-check-again

Id Subject Object Predicate Lexical cue
T18109 776-779 Protein denotes CD8
T18110 869-873 Protein denotes IL-2

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T18111 776-780 Protein denotes CD8+

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T17560 776-779 Protein denotes CD8
T17561 869-873 Protein denotes IL-2

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T18072 776-779 P10966 denotes CD8
T18073 776-779 P01732 denotes CD8
T18074 869-873 P60568 denotes IL-2
T18075 905-907 P0A7Z4 denotes pH

test2

Id Subject Object Predicate Lexical cue
T17558 776-779 Protein denotes CD8
T17559 869-873 Protein denotes IL-2