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PMC:2626671 / 31057-33611 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T16963 0-4 NN denotes ChIP
T16964 5-8 CC denotes and
T16965 9-18 JJ denotes real-time
T16966 19-22 NN denotes PCR
T16967 23-31 NN denotes analysis
T16968 33-35 CD denotes 20
T16969 36-37 CC denotes ×
T16970 38-41 CD denotes 106
T16971 42-46 JJ denotes CD8+
T16972 47-48 NN denotes T
T16973 49-54 NN denotes cells
T16974 55-58 IN denotes per
T16975 59-78 NN denotes immunoprecipitation
T16976 79-83 VB denotes were
T16977 84-89 VB denotes fixed
T16978 90-92 IN denotes by
T16979 93-99 VB denotes adding
T16980 100-101 DT denotes a
T16981 102-108 JJ denotes 1/10th
T16982 109-115 NN denotes volume
T16983 116-118 IN denotes of
T16984 119-127 NN denotes fixation
T16985 128-136 NN denotes solution
T16986 137-138 -LRB- denotes (
T16987 138-142 CD denotes 11.1
T16988 142-143 NN denotes %
T16989 144-156 NN denotes formaldehyde
T16990 156-157 -COMMA- denotes ,
T16991 158-161 CD denotes 100
T16992 162-164 NN denotes mM
T16993 165-169 NN denotes NaCl
T16994 169-170 -COMMA- denotes ,
T16995 171-172 CD denotes 1
T16996 173-175 NN denotes mM
T16997 176-180 NN denotes EDTA
T16998 180-181 -COMMA- denotes ,
T16999 182-185 CD denotes 0.5
T17000 186-188 NN denotes mM
T17001 189-193 NN denotes EGTA
T17002 193-194 -COMMA- denotes ,
T17003 195-197 CD denotes 50
T17004 198-200 NN denotes mM
T17005 201-206 NN denotes Hepes
T17006 206-207 -RRB- denotes )
T17007 208-210 TO denotes to
T17008 211-212 CD denotes 1
T17009 213-219 NN denotes volume
T17010 220-222 IN denotes of
T17011 223-230 NN denotes culture
T17012 231-236 NN denotes media
T17013 237-240 CC denotes and
T17014 241-245 VB denotes were
T17015 246-255 VB denotes incubated
T17016 256-259 IN denotes for
T17017 260-262 CD denotes 10
T17018 263-265 CC denotes or
T17019 266-268 CD denotes 30
T17020 269-272 NN denotes min
T17021 273-275 IN denotes at
T17022 276-278 NN denotes RT
T17023 280-288 NN denotes Fixation
T17024 289-292 VB denotes was
T17025 293-300 VB denotes stopped
T17026 301-305 IN denotes with
T17027 306-309 CD denotes 120
T17028 310-312 NN denotes mM
T17029 313-320 NN denotes glycine
T17030 321-323 IN denotes on
T17031 324-327 NN denotes ice
T17032 328-331 IN denotes for
T17033 332-333 CD denotes 5
T17034 334-337 NN denotes min
T17035 339-344 VB denotes Fixed
T17036 345-350 NN denotes cells
T17037 351-355 VB denotes were
T17038 356-362 VB denotes washed
T17039 363-365 CD denotes 2×
T17040 366-370 IN denotes with
T17041 371-375 JJ denotes cold
T17042 376-379 NNP denotes PBS
T17043 379-380 -COMMA- denotes ,
T17044 381-383 CD denotes 1×
T17045 384-388 IN denotes with
T17046 389-393 JJ denotes cold
T17047 394-402 NN denotes solution
T17048 403-404 CD denotes I
T17049 405-406 -LRB- denotes (
T17050 406-408 CD denotes 10
T17051 409-411 NN denotes mM
T17052 412-416 NN denotes Tris
T17053 417-418 -LRB- denotes [
T17054 418-420 NN denotes pH
T17055 421-424 CD denotes 7.5
T17056 424-425 -RRB- denotes ]
T17057 425-426 -COMMA- denotes ,
T17058 427-429 CD denotes 10
T17059 430-432 NN denotes mM
T17060 433-437 NN denotes EDTA
T17061 437-438 -COMMA- denotes ,
T17062 439-442 CD denotes 0.5
T17063 443-445 NN denotes mM
T17064 446-450 NN denotes EGTA
T17065 450-451 -COMMA- denotes ,
T17066 452-453 CD denotes 1
T17067 453-454 NN denotes %
T17068 455-461 NN denotes Triton
T17069 462-467 NN denotes X-100
T17070 467-468 -RRB- denotes )
T17071 468-469 -COMMA- denotes ,
T17072 470-473 CC denotes and
T17073 474-476 CD denotes 1×
T17074 477-481 IN denotes with
T17075 482-486 JJ denotes cold
T17076 487-495 NN denotes solution
T17077 496-498 CD denotes II
T17078 499-500 -LRB- denotes (
T17079 500-502 CD denotes 10
T17080 503-505 NN denotes mM
T17081 506-510 NN denotes Tris
T17082 511-512 -LRB- denotes [
T17083 512-514 NN denotes pH
T17084 515-518 CD denotes 7.5
T17085 518-519 -RRB- denotes ]
T17086 519-520 -COMMA- denotes ,
T17087 521-522 CD denotes 1
T17088 523-525 NN denotes mM
T17089 526-530 NN denotes EDTA
T17090 530-531 -COMMA- denotes ,
T17091 532-535 CD denotes 0.5
T17092 536-538 NN denotes mM
T17093 539-543 NN denotes EGTA
T17094 543-544 -COMMA- denotes ,
T17095 545-548 CD denotes 200
T17096 549-551 NN denotes mM
T17097 552-556 NN denotes NaCl
T17098 556-557 -RRB- denotes )
T17099 559-564 IN denotes After
T17100 565-571 NN denotes washes
T17101 571-572 -COMMA- denotes ,
T17102 573-577 NN denotes cell
T17103 578-585 NN denotes pellets
T17104 586-590 VB denotes were
T17105 591-602 VB denotes resuspended
T17106 603-605 IN denotes at
T17107 606-608 CD denotes 40
T17108 609-610 CC denotes ×
T17109 611-614 CD denotes 106
T17110 615-623 NN denotes cells/ml
T17111 624-626 IN denotes in
T17112 627-631 NN denotes ChIP
T17113 632-637 NN denotes lysis
T17114 638-644 NN denotes buffer
T17115 645-646 -LRB- denotes (
T17116 646-649 CD denotes 150
T17117 650-652 NN denotes mM
T17118 653-657 NN denotes NaCl
T17119 657-658 -COMMA- denotes ,
T17120 659-661 CD denotes 25
T17121 662-664 NN denotes mM
T17122 665-669 NN denotes Tris
T17123 670-671 -LRB- denotes [
T17124 671-673 NN denotes pH
T17125 674-677 CD denotes 7.5
T17126 677-678 -RRB- denotes ]
T17127 678-679 -COMMA- denotes ,
T17128 680-681 CD denotes 1
T17129 681-682 NN denotes %
T17130 683-689 NN denotes Triton
T17131 690-695 NN denotes X-100
T17132 695-696 -COMMA- denotes ,
T17133 697-700 CD denotes 0.1
T17134 700-701 NN denotes %
T17135 702-705 NN denotes SDS
T17136 705-706 -COMMA- denotes ,
T17137 707-710 CD denotes 0.5
T17138 710-711 NN denotes %
T17139 712-724 NN denotes deoxycholate
T17140 725-729 CC denotes plus
T17141 730-738 NN denotes protease
T17142 739-742 CC denotes and
T17143 743-754 NN denotes phosphatase
T17144 755-765 NN denotes inhibitors
T17145 765-766 -RRB- denotes )
T17146 766-767 -COMMA- denotes ,
T17147 768-771 CC denotes and
T17148 772-781 NN denotes chromatin
T17149 782-785 VB denotes was
T17150 786-793 VB denotes sheared
T17151 794-798 IN denotes with
T17152 799-800 DT denotes a
T17153 801-810 NN denotes sonicator
T17154 811-813 TO denotes to
T17155 814-819 VB denotes yield
T17156 820-828 JJ denotes 0.5–1-kb
T17157 829-832 NN denotes DNA
T17158 833-842 NN denotes fragments
T17159 844-849 IN denotes After
T17160 850-861 VB denotes preclearing
T17161 862-865 DT denotes the
T17162 866-873 JJ denotes sheared
T17163 874-883 NN denotes chromatin
T17164 884-888 IN denotes with
T17165 889-896 NN denotes protein
T17166 897-908 NN denotes A–sepharose
T17167 909-914 NN denotes beads
T17168 915-918 CC denotes and
T17169 919-927 VB denotes removing
T17170 928-929 CD denotes 5
T17171 929-930 NN denotes %
T17172 931-933 IN denotes as
T17173 934-939 NN denotes input
T17174 940-949 NN denotes chromatin
T17175 949-950 -COMMA- denotes ,
T17176 951-970 NN denotes immunoprecipitation
T17177 971-974 VB denotes was
T17178 975-984 VB denotes performed
T17179 985-987 IN denotes by
T17180 988-994 VB denotes adding
T17181 995-1004 VB denotes optimized
T17182 1005-1013 NN denotes antibody
T17183 1014-1021 NN denotes amounts
T17184 1022-1023 -LRB- denotes (
T17185 1023-1026 IN denotes per
T17186 1027-1029 CD denotes 20
T17187 1030-1031 -COLON- denotes ×
T17188 1032-1035 CD denotes 106
T17189 1036-1040 NN denotes cell
T17190 1041-1052 NN denotes equivalents
T17191 1052-1053 -COLON- denotes :
T17192 1054-1057 CD denotes 2.5
T17193 1058-1060 NN denotes μg
T17194 1061-1071 NN denotes anti-Eomes
T17195 1071-1072 -COMMA- denotes ,
T17196 1073-1074 CD denotes 1
T17197 1074-1075 SYM denotes :
T17198 1075-1078 CD denotes 100
T17199 1079-1087 NN denotes dilution
T17200 1088-1098 NN denotes anti-Runx3
T17201 1098-1099 -RRB- denotes )
T17202 1099-1100 -COMMA- denotes ,
T17203 1101-1109 VB denotes followed
T17204 1110-1112 IN denotes by
T17205 1113-1122 JJ denotes overnight
T17206 1123-1133 NN denotes incubation
T17207 1134-1136 IN denotes at
T17208 1137-1140 NN denotes 4°C
T17209 1140-1141 -COLON- denotes ;
T17210 1142-1149 NN denotes protein
T17211 1150-1161 NN denotes A–sepharose
T17212 1162-1167 NN denotes beads
T17213 1168-1172 VB denotes were
T17214 1173-1178 VB denotes added
T17215 1179-1182 IN denotes for
T17216 1183-1186 DT denotes the
T17217 1187-1191 JJ denotes last
T17218 1192-1193 CD denotes 3
T17219 1194-1195 NN denotes h
T17220 1196-1198 IN denotes of
T17221 1199-1202 DT denotes the
T17222 1203-1213 NN denotes incubation
T17223 1214-1220 NN denotes period
T17224 1222-1227 NN denotes Beads
T17225 1228-1232 VB denotes were
T17226 1233-1239 VB denotes washed
T17227 1240-1242 CD denotes 2×
T17228 1243-1247 IN denotes with
T17229 1248-1252 NN denotes RIPA
T17230 1253-1259 NN denotes buffer
T17231 1260-1261 -LRB- denotes (
T17232 1261-1263 CD denotes 50
T17233 1264-1266 NN denotes mM
T17234 1267-1271 NN denotes Tris
T17235 1272-1273 -LRB- denotes [
T17236 1273-1275 NN denotes pH
T17237 1276-1277 CD denotes 8
T17238 1277-1278 -RRB- denotes ]
T17239 1278-1279 -COMMA- denotes ,
T17240 1280-1283 CD denotes 150
T17241 1284-1286 NN denotes mM
T17242 1287-1291 NN denotes NaCl
T17243 1291-1292 -COMMA- denotes ,
T17244 1293-1294 CD denotes 1
T17245 1295-1297 NN denotes mM
T17246 1298-1302 NN denotes EDTA
T17247 1302-1303 -COMMA- denotes ,
T17248 1304-1305 CD denotes 1
T17249 1305-1306 NN denotes %
T17250 1307-1312 NN denotes NP-40
T17251 1312-1313 -COMMA- denotes ,
T17252 1314-1317 CD denotes 0.1
T17253 1317-1318 NN denotes %
T17254 1319-1322 NN denotes SDS
T17255 1322-1323 -COMMA- denotes ,
T17256 1324-1327 CD denotes 0.5
T17257 1327-1328 NN denotes %
T17258 1329-1341 NN denotes deoxycholate
T17259 1341-1342 -RRB- denotes )
T17260 1342-1343 -COMMA- denotes ,
T17261 1344-1346 CD denotes 1×
T17262 1347-1351 IN denotes with
T17263 1352-1356 JJ denotes high
T17264 1357-1361 NN denotes salt
T17265 1362-1368 NN denotes buffer
T17266 1369-1370 -LRB- denotes (
T17267 1370-1372 CD denotes 50
T17268 1373-1375 NN denotes mM
T17269 1376-1380 NN denotes Tris
T17270 1381-1382 -LRB- denotes [
T17271 1382-1384 NN denotes pH
T17272 1385-1386 CD denotes 8
T17273 1386-1387 -RRB- denotes ]
T17274 1387-1388 -COMMA- denotes ,
T17275 1389-1392 CD denotes 500
T17276 1393-1395 NN denotes mM
T17277 1396-1400 NN denotes NaCl
T17278 1400-1401 -COMMA- denotes ,
T17279 1402-1403 CD denotes 1
T17280 1404-1406 NN denotes mM
T17281 1407-1411 NN denotes EDTA
T17282 1411-1412 -COMMA- denotes ,
T17283 1413-1414 CD denotes 1
T17284 1414-1415 NN denotes %
T17285 1416-1421 NN denotes NP-40
T17286 1421-1422 -COMMA- denotes ,
T17287 1423-1426 CD denotes 0.1
T17288 1426-1427 NN denotes %
T17289 1428-1431 NN denotes SDS
T17290 1431-1432 -RRB- denotes )
T17291 1432-1433 -COMMA- denotes ,
T17292 1434-1437 CC denotes and
T17293 1438-1440 NN denotes 1×
T17294 1441-1445 IN denotes with
T17295 1446-1448 NN denotes TE
T17296 1449-1455 NN denotes buffer
T17297 1457-1462 IN denotes After
T17298 1463-1466 DT denotes the
T17299 1467-1471 JJ denotes last
T17300 1472-1476 NN denotes wash
T17301 1476-1477 -COMMA- denotes ,
T17302 1478-1481 NN denotes DNA
T17303 1482-1485 VB denotes was
T17304 1486-1492 VB denotes eluted
T17305 1493-1495 IN denotes by
T17306 1496-1508 VB denotes resuspending
T17307 1509-1512 DT denotes the
T17308 1513-1518 NN denotes beads
T17309 1519-1521 IN denotes in
T17310 1522-1529 NN denotes elution
T17311 1530-1536 NN denotes buffer
T17312 1537-1538 -LRB- denotes (
T17313 1538-1539 CD denotes 1
T17314 1539-1540 NN denotes %
T17315 1541-1544 NN denotes SDS
T17316 1544-1545 -COMMA- denotes ,
T17317 1546-1549 CD denotes 100
T17318 1550-1552 NN denotes mM
T17319 1553-1559 NN denotes NaHCO3
T17320 1559-1560 -RRB- denotes )
T17321 1562-1566 CC denotes Both
T17322 1567-1572 NN denotes input
T17323 1573-1576 CC denotes and
T17324 1577-1581 NN denotes ChIP
T17325 1582-1591 NN denotes chromatin
T17326 1592-1596 VB denotes were
T17327 1597-1601 RB denotes then
T17328 1602-1609 VB denotes treated
T17329 1610-1614 IN denotes with
T17330 1615-1620 NN denotes RNase
T17331 1621-1622 NN denotes A
T17332 1623-1624 -LRB- denotes (
T17333 1624-1625 CD denotes 5
T17334 1626-1628 NN denotes μg
T17335 1629-1634 NN denotes total
T17336 1634-1635 -RRB- denotes )
T17337 1636-1639 IN denotes for
T17338 1640-1641 CD denotes 1
T17339 1642-1643 NN denotes h
T17340 1644-1646 IN denotes at
T17341 1647-1651 NN denotes 37°C
T17342 1651-1652 -COMMA- denotes ,
T17343 1653-1661 VB denotes followed
T17344 1662-1664 IN denotes by
T17345 1665-1668 DT denotes the
T17346 1669-1677 NN denotes addition
T17347 1678-1680 IN denotes of
T17348 1681-1691 NN denotes proteinase
T17349 1692-1693 NN denotes K
T17350 1694-1695 -LRB- denotes (
T17351 1695-1698 CD denotes 100
T17352 1699-1701 NN denotes μg
T17353 1702-1707 NN denotes total
T17354 1707-1708 -RRB- denotes )
T17355 1709-1712 CC denotes and
T17356 1713-1722 JJ denotes overnight
T17357 1723-1733 NN denotes incubation
T17358 1734-1736 IN denotes at
T17359 1737-1741 NN denotes 65°C
T17360 1742-1744 TO denotes to
T17361 1745-1752 VB denotes reverse
T17362 1753-1766 NN denotes cross-linking
T17363 1768-1771 NN denotes DNA
T17364 1772-1775 VB denotes was
T17365 1776-1780 RB denotes then
T17366 1781-1789 VB denotes purified
T17367 1790-1794 IN denotes with
T17368 1795-1803 JJ denotes QIAquick
T17369 1804-1811 NN denotes columns
T17370 1812-1813 -LRB- denotes (
T17371 1813-1816 NN denotes Gel
T17372 1817-1827 NN denotes Extraction
T17373 1828-1831 NN denotes Kit
T17374 1831-1832 -COLON- denotes ;
T17375 1833-1839 NN denotes QIAGEN
T17376 1839-1840 -RRB- denotes )
T17377 1841-1850 VB denotes according
T17378 1851-1853 TO denotes to
T17379 1854-1857 DT denotes the
T17380 1858-1870 NN denotes manufacturer
T17381 1870-1872 POS denotes 's
T17382 1873-1885 NN denotes instructions
T17383 1886-1889 CC denotes and
T17384 1890-1901 VB denotes resuspended
T17385 1902-1904 IN denotes in
T17386 1905-1906 DT denotes a
T17387 1907-1912 JJ denotes 50-μl
T17388 1913-1919 NN denotes volume
T17389 1921-1924 IN denotes For
T17390 1925-1934 JJ denotes real-time
T17391 1935-1938 NN denotes PCR
T17392 1939-1948 NN denotes detection
T17393 1949-1951 IN denotes of
T17394 1952-1970 JJ denotes immunoprecipitated
T17395 1971-1978 NN denotes targets
T17396 1979-1984 VB denotes using
T17397 1985-1988 DT denotes the
T17398 1989-1993 NNP denotes SYBR
T17399 1994-1999 NNP denotes Green
T17400 2000-2003 NN denotes PCR
T17401 2004-2007 NN denotes Kit
T17402 2007-2008 -COMMA- denotes ,
T17403 2009-2010 DT denotes a
T17404 2011-2019 JJ denotes standard
T17405 2020-2025 NN denotes curve
T17406 2026-2029 VB denotes was
T17407 2030-2038 VB denotes obtained
T17408 2039-2043 IN denotes with
T17409 2044-2050 JJ denotes serial
T17410 2051-2060 NN denotes dilutions
T17411 2061-2063 IN denotes of
T17412 2064-2069 NN denotes input
T17413 2070-2073 NN denotes DNA
T17414 2074-2077 IN denotes for
T17415 2078-2082 DT denotes each
T17416 2083-2089 NN denotes sample
T17417 2089-2090 -COMMA- denotes ,
T17418 2091-2094 CC denotes and
T17419 2095-2096 CD denotes 1
T17420 2097-2099 NN denotes μl
T17421 2100-2104 NN denotes ChIP
T17422 2105-2108 NN denotes DNA
T17423 2109-2112 VB denotes was
T17424 2113-2117 VB denotes used
T17425 2118-2121 IN denotes per
T17426 2122-2125 NN denotes PCR
T17427 2126-2134 NN denotes reaction
T17428 2135-2136 -LRB- denotes (
T17429 2136-2145 VB denotes performed
T17430 2146-2148 IN denotes in
T17431 2149-2159 NN denotes duplicates
T17432 2159-2160 -RRB- denotes )
T17433 2162-2166 NN denotes Melt
T17434 2167-2173 NN denotes curves
T17435 2174-2177 CC denotes and
T17436 2178-2185 NN denotes agarose
T17437 2186-2190 NN denotes gels
T17438 2191-2195 VB denotes were
T17439 2196-2204 VB denotes analyzed
T17440 2205-2207 TO denotes to
T17441 2208-2214 VB denotes ensure
T17442 2215-2228 NN denotes amplification
T17443 2229-2231 IN denotes of
T17444 2232-2240 JJ denotes specific
T17445 2241-2247 NN denotes target
T17446 2248-2257 NN denotes sequences
T17447 2259-2264 VB denotes Refer
T17448 2265-2267 TO denotes to
T17449 2268-2273 JJ denotes Table
T17450 2274-2276 NN denotes S1
T17451 2277-2278 -LRB- denotes (
T17452 2278-2287 JJ denotes available
T17453 2288-2290 IN denotes at
T17454 2291-2295 NN denotes http
T17455 2295-2296 -COLON- denotes :
T17456 2296-2343 NN denotes //www.jem.org/cgi/content/full/jem.20081242/DC1
T17457 2343-2344 -RRB- denotes )
T17458 2345-2348 IN denotes for
T17459 2349-2350 DT denotes a
T17460 2351-2355 NN denotes list
T17461 2356-2358 IN denotes of
T17462 2359-2365 NN denotes primer
T17463 2366-2370 NN denotes sets
T17464 2372-2375 DT denotes The
T17465 2376-2380 NN denotes data
T17466 2381-2384 VB denotes are
T17467 2385-2394 VB denotes presented
T17468 2395-2397 IN denotes as
T17469 2398-2401 DT denotes the
T17470 2402-2408 NN denotes number
T17471 2409-2411 IN denotes of
T17472 2412-2430 VB denotes immunoprecipitated
T17473 2431-2437 NN denotes target
T17474 2438-2447 NN denotes sequences
T17475 2448-2456 JJ denotes relative
T17476 2457-2459 TO denotes to
T17477 2460-2465 NN denotes input
T17478 2466-2475 NN denotes chromatin
T17479 2475-2476 -COMMA- denotes ,
T17480 2477-2485 VB denotes assuming
T17481 2486-2489 CD denotes two
T17482 2490-2496 NN denotes copies
T17483 2497-2499 IN denotes of
T17484 2500-2506 NN denotes target
T17485 2507-2515 NN denotes sequence
T17486 2516-2519 IN denotes per
T17487 2520-2524 NN denotes cell
T17488 2525-2535 JJ denotes equivalent
T17489 2536-2540 VB denotes used
T17490 2541-2544 IN denotes for
T17491 2545-2548 DT denotes the
T17492 2549-2553 NN denotes ChIP
R13047 T16967 T16963 arg1Of analysis,ChIP
R13048 T16967 T16964 arg1Of analysis,and
R13049 T16967 T16965 arg1Of analysis,real-time
R13050 T16967 T16966 arg1Of analysis,PCR
R13051 T16968 T16969 arg1Of 20,×
R13052 T16970 T16969 arg2Of 106,×
R13053 T16973 T16968 arg1Of cells,20
R13054 T16973 T16970 arg1Of cells,106
R13055 T16973 T16971 arg1Of cells,CD8+
R13056 T16973 T16972 arg1Of cells,T
R13057 T16973 T16974 arg1Of cells,per
R13058 T16973 T16976 arg1Of cells,were
R13059 T16973 T16977 arg2Of cells,fixed
R13060 T16973 T17014 arg1Of cells,were
R13061 T16973 T17015 arg2Of cells,incubated
R13062 T16975 T16974 arg2Of immunoprecipitation,per
R13063 T16977 T16976 arg2Of fixed,were
R13064 T16977 T16978 arg1Of fixed,by
R13065 T16977 T17013 arg1Of fixed,and
R13066 T16979 T16978 arg2Of adding,by
R13067 T16982 T16979 arg2Of volume,adding
R13068 T16982 T16980 arg1Of volume,a
R13069 T16982 T16981 arg1Of volume,1/10th
R13070 T16982 T16983 arg1Of volume,of
R13071 T16982 T16986 arg1Of volume,(
R13072 T16982 T17007 arg1Of volume,to
R13073 T16985 T16983 arg2Of solution,of
R13074 T16985 T16984 arg1Of solution,fixation
R13075 T16987 T16988 arg1Of 11.1,%
R13076 T16989 T16987 arg1Of formaldehyde,11.1
R13077 T16989 T16990 arg1Of formaldehyde,","
R13078 T16990 T16994 arg1Of ",",","
R13079 T16991 T16992 arg1Of 100,mM
R13080 T16993 T16990 arg2Of NaCl,","
R13081 T16993 T16991 arg1Of NaCl,100
R13082 T16994 T16998 arg1Of ",",","
R13083 T16995 T16996 arg1Of 1,mM
R13084 T16997 T16994 arg2Of EDTA,","
R13085 T16997 T16995 arg1Of EDTA,1
R13086 T16998 T17002 arg1Of ",",","
R13087 T16999 T17000 arg1Of 0.5,mM
R13088 T17001 T16998 arg2Of EGTA,","
R13089 T17001 T16999 arg1Of EGTA,0.5
R13090 T17002 T16986 arg2Of ",",(
R13091 T17003 T17004 arg1Of 50,mM
R13092 T17005 T17002 arg2Of Hepes,","
R13093 T17005 T17003 arg1Of Hepes,50
R13094 T17006 T16986 arg3Of ),(
R13095 T17009 T17007 arg2Of volume,to
R13096 T17009 T17008 arg1Of volume,1
R13097 T17009 T17010 arg1Of volume,of
R13098 T17012 T17010 arg2Of media,of
R13099 T17012 T17011 arg1Of media,culture
R13100 T17015 T17013 arg2Of incubated,and
R13101 T17015 T17014 arg2Of incubated,were
R13102 T17015 T17016 arg1Of incubated,for
R13103 T17017 T17018 arg1Of 10,or
R13104 T17019 T17018 arg2Of 30,or
R13105 T17020 T17016 arg2Of min,for
R13106 T17020 T17017 arg1Of min,10
R13107 T17020 T17019 arg1Of min,30
R13108 T17020 T17021 arg1Of min,at
R13109 T17022 T17021 arg2Of RT,at
R13110 T17023 T17024 arg1Of Fixation,was
R13111 T17023 T17025 arg2Of Fixation,stopped
R13112 T17025 T17024 arg2Of stopped,was
R13113 T17025 T17026 arg1Of stopped,with
R13114 T17025 T17032 arg1Of stopped,for
R13115 T17027 T17028 arg1Of 120,mM
R13116 T17029 T17026 arg2Of glycine,with
R13117 T17029 T17027 arg1Of glycine,120
R13118 T17029 T17030 arg1Of glycine,on
R13119 T17031 T17030 arg2Of ice,on
R13120 T17034 T17032 arg2Of min,for
R13121 T17034 T17033 arg1Of min,5
R13122 T17036 T17035 arg2Of cells,Fixed
R13123 T17036 T17037 arg1Of cells,were
R13124 T17036 T17038 arg2Of cells,washed
R13125 T17038 T17037 arg2Of washed,were
R13126 T17039 T17040 arg1Of 2×,with
R13127 T17039 T17072 arg1Of 2×,and
R13128 T17042 T17040 arg2Of PBS,with
R13129 T17042 T17041 arg1Of PBS,cold
R13130 T17042 T17043 arg1Of PBS,","
R13131 T17044 T17043 arg2Of 1×,","
R13132 T17044 T17045 arg1Of 1×,with
R13133 T17047 T17045 arg2Of solution,with
R13134 T17047 T17046 arg1Of solution,cold
R13135 T17047 T17048 arg1Of solution,I
R13136 T17047 T17049 arg1Of solution,(
R13137 T17050 T17051 arg1Of 10,mM
R13138 T17052 T17050 arg1Of Tris,10
R13139 T17052 T17053 arg1Of Tris,[
R13140 T17052 T17057 arg1Of Tris,","
R13141 T17054 T17053 arg2Of pH,[
R13142 T17054 T17055 arg1Of pH,7.5
R13143 T17056 T17053 arg3Of ],[
R13144 T17057 T17061 arg1Of ",",","
R13145 T17058 T17059 arg1Of 10,mM
R13146 T17060 T17057 arg2Of EDTA,","
R13147 T17060 T17058 arg1Of EDTA,10
R13148 T17061 T17065 arg1Of ",",","
R13149 T17062 T17063 arg1Of 0.5,mM
R13150 T17064 T17061 arg2Of EGTA,","
R13151 T17064 T17062 arg1Of EGTA,0.5
R13152 T17065 T17049 arg2Of ",",(
R13153 T17066 T17067 arg1Of 1,%
R13154 T17069 T17065 arg2Of X-100,","
R13155 T17069 T17066 arg1Of X-100,1
R13156 T17069 T17068 arg1Of X-100,Triton
R13157 T17070 T17049 arg3Of ),(
R13158 T17072 T17038 arg3Of and,washed
R13159 T17072 T17071 arg1Of and,","
R13160 T17073 T17072 arg2Of 1×,and
R13161 T17073 T17074 arg1Of 1×,with
R13162 T17076 T17074 arg2Of solution,with
R13163 T17076 T17075 arg1Of solution,cold
R13164 T17076 T17077 arg1Of solution,II
R13165 T17076 T17078 arg1Of solution,(
R13166 T17079 T17080 arg1Of 10,mM
R13167 T17081 T17079 arg1Of Tris,10
R13168 T17081 T17082 arg1Of Tris,[
R13169 T17081 T17086 arg1Of Tris,","
R13170 T17083 T17082 arg2Of pH,[
R13171 T17083 T17084 arg1Of pH,7.5
R13172 T17085 T17082 arg3Of ],[
R13173 T17086 T17090 arg1Of ",",","
R13174 T17087 T17088 arg1Of 1,mM
R13175 T17089 T17086 arg2Of EDTA,","
R13176 T17089 T17087 arg1Of EDTA,1
R13177 T17090 T17094 arg1Of ",",","
R13178 T17091 T17092 arg1Of 0.5,mM
R13179 T17093 T17090 arg2Of EGTA,","
R13180 T17093 T17091 arg1Of EGTA,0.5
R13181 T17094 T17078 arg2Of ",",(
R13182 T17095 T17096 arg1Of 200,mM
R13183 T17097 T17094 arg2Of NaCl,","
R13184 T17097 T17095 arg1Of NaCl,200
R13185 T17098 T17078 arg3Of ),(
R13186 T17100 T17099 arg2Of washes,After
R13187 T17103 T17102 arg1Of pellets,cell
R13188 T17103 T17104 arg1Of pellets,were
R13189 T17103 T17105 arg2Of pellets,resuspended
R13190 T17105 T17099 arg1Of resuspended,After
R13191 T17105 T17101 arg1Of resuspended,","
R13192 T17105 T17104 arg2Of resuspended,were
R13193 T17105 T17106 arg1Of resuspended,at
R13194 T17105 T17111 arg1Of resuspended,in
R13195 T17105 T17147 arg1Of resuspended,and
R13196 T17107 T17108 arg1Of 40,×
R13197 T17109 T17108 arg2Of 106,×
R13198 T17110 T17106 arg2Of cells/ml,at
R13199 T17110 T17107 arg1Of cells/ml,40
R13200 T17110 T17109 arg1Of cells/ml,106
R13201 T17114 T17111 arg2Of buffer,in
R13202 T17114 T17112 arg1Of buffer,ChIP
R13203 T17114 T17113 arg1Of buffer,lysis
R13204 T17114 T17115 arg1Of buffer,(
R13205 T17118 T17116 arg1Of NaCl,150
R13206 T17118 T17117 arg1Of NaCl,mM
R13207 T17118 T17119 arg1Of NaCl,","
R13208 T17119 T17127 arg1Of ",",","
R13209 T17120 T17121 arg1Of 25,mM
R13210 T17122 T17119 arg2Of Tris,","
R13211 T17122 T17120 arg1Of Tris,25
R13212 T17122 T17123 arg1Of Tris,[
R13213 T17124 T17123 arg2Of pH,[
R13214 T17124 T17125 arg1Of pH,7.5
R13215 T17126 T17123 arg3Of ],[
R13216 T17127 T17132 arg1Of ",",","
R13217 T17128 T17129 arg1Of 1,%
R13218 T17131 T17127 arg2Of X-100,","
R13219 T17131 T17128 arg1Of X-100,1
R13220 T17131 T17130 arg1Of X-100,Triton
R13221 T17132 T17136 arg1Of ",",","
R13222 T17133 T17134 arg1Of 0.1,%
R13223 T17135 T17132 arg2Of SDS,","
R13224 T17135 T17133 arg1Of SDS,0.1
R13225 T17136 T17142 arg1Of ",",and
R13226 T17137 T17138 arg1Of 0.5,%
R13227 T17139 T17140 arg1Of deoxycholate,plus
R13228 T17140 T17136 arg2Of plus,","
R13229 T17140 T17137 arg1Of plus,0.5
R13230 T17141 T17140 arg2Of protease,plus
R13231 T17142 T17115 arg2Of and,(
R13232 T17144 T17142 arg2Of inhibitors,and
R13233 T17144 T17143 arg1Of inhibitors,phosphatase
R13234 T17145 T17115 arg3Of ),(
R13235 T17147 T17146 arg1Of and,","
R13236 T17148 T17149 arg1Of chromatin,was
R13237 T17148 T17150 arg2Of chromatin,sheared
R13238 T17150 T17147 arg2Of sheared,and
R13239 T17150 T17149 arg2Of sheared,was
R13240 T17150 T17151 arg1Of sheared,with
R13241 T17153 T17151 arg2Of sonicator,with
R13242 T17153 T17152 arg1Of sonicator,a
R13243 T17153 T17154 modOf sonicator,to
R13244 T17155 T17154 arg1Of yield,to
R13245 T17158 T17155 arg2Of fragments,yield
R13246 T17158 T17156 arg1Of fragments,0.5–1-kb
R13247 T17158 T17157 arg1Of fragments,DNA
R13248 T17160 T17168 arg1Of preclearing,and
R13249 T17163 T17160 arg2Of chromatin,preclearing
R13250 T17163 T17161 arg1Of chromatin,the
R13251 T17163 T17162 arg1Of chromatin,sheared
R13252 T17163 T17164 arg1Of chromatin,with
R13253 T17167 T17164 arg2Of beads,with
R13254 T17167 T17165 arg1Of beads,protein
R13255 T17167 T17166 arg1Of beads,A–sepharose
R13256 T17168 T17159 arg2Of and,After
R13257 T17169 T17168 arg2Of removing,and
R13258 T17169 T17172 arg1Of removing,as
R13259 T17171 T17169 arg2Of %,removing
R13260 T17171 T17170 arg1Of %,5
R13261 T17174 T17172 arg2Of chromatin,as
R13262 T17174 T17173 arg1Of chromatin,input
R13263 T17176 T17160 arg1Of immunoprecipitation,preclearing
R13264 T17176 T17169 arg1Of immunoprecipitation,removing
R13265 T17176 T17177 arg1Of immunoprecipitation,was
R13266 T17176 T17178 arg2Of immunoprecipitation,performed
R13267 T17178 T17159 arg1Of performed,After
R13268 T17178 T17175 arg1Of performed,","
R13269 T17178 T17177 arg2Of performed,was
R13270 T17178 T17179 arg1Of performed,by
R13271 T17180 T17179 arg2Of adding,by
R13272 T17183 T17180 arg2Of amounts,adding
R13273 T17183 T17181 arg2Of amounts,optimized
R13274 T17183 T17182 arg1Of amounts,antibody
R13275 T17183 T17184 arg1Of amounts,(
R13276 T17185 T17184 arg2Of per,(
R13277 T17190 T17185 arg2Of equivalents,per
R13278 T17190 T17186 arg1Of equivalents,20
R13279 T17190 T17187 arg1Of equivalents,×
R13280 T17190 T17188 arg1Of equivalents,106
R13281 T17190 T17189 arg1Of equivalents,cell
R13282 T17191 T17184 arg3Of :,(
R13283 T17194 T17192 arg1Of anti-Eomes,2.5
R13284 T17194 T17193 arg1Of anti-Eomes,μg
R13285 T17194 T17195 arg1Of anti-Eomes,","
R13286 T17195 T17202 arg1Of ",",","
R13287 T17195 T17203 arg2Of ",",followed
R13288 T17198 T17197 arg1Of 100,:
R13289 T17200 T17195 arg2Of anti-Runx3,","
R13290 T17200 T17196 arg1Of anti-Runx3,1
R13291 T17200 T17198 arg1Of anti-Runx3,100
R13292 T17200 T17199 arg1Of anti-Runx3,dilution
R13293 T17200 T17201 arg1Of anti-Runx3,)
R13294 T17203 T17209 arg1Of followed,;
R13295 T17206 T17203 arg1Of incubation,followed
R13296 T17206 T17204 arg2Of incubation,by
R13297 T17206 T17205 arg1Of incubation,overnight
R13298 T17206 T17207 arg1Of incubation,at
R13299 T17208 T17207 arg2Of 4°C,at
R13300 T17212 T17210 arg1Of beads,protein
R13301 T17212 T17211 arg1Of beads,A–sepharose
R13302 T17212 T17213 arg1Of beads,were
R13303 T17212 T17214 arg2Of beads,added
R13304 T17214 T17209 arg2Of added,;
R13305 T17214 T17213 arg2Of added,were
R13306 T17214 T17215 arg1Of added,for
R13307 T17219 T17215 arg2Of h,for
R13308 T17219 T17216 arg1Of h,the
R13309 T17219 T17217 arg1Of h,last
R13310 T17219 T17218 arg1Of h,3
R13311 T17219 T17220 arg1Of h,of
R13312 T17223 T17220 arg2Of period,of
R13313 T17223 T17221 arg1Of period,the
R13314 T17223 T17222 arg1Of period,incubation
R13315 T17224 T17225 arg1Of Beads,were
R13316 T17224 T17226 arg2Of Beads,washed
R13317 T17226 T17225 arg2Of washed,were
R13318 T17227 T17228 arg1Of 2×,with
R13319 T17227 T17260 arg1Of 2×,","
R13320 T17230 T17228 arg2Of buffer,with
R13321 T17230 T17229 arg1Of buffer,RIPA
R13322 T17230 T17231 arg1Of buffer,(
R13323 T17232 T17233 arg1Of 50,mM
R13324 T17234 T17232 arg1Of Tris,50
R13325 T17234 T17235 arg1Of Tris,[
R13326 T17234 T17239 arg1Of Tris,","
R13327 T17236 T17235 arg2Of pH,[
R13328 T17236 T17237 arg1Of pH,8
R13329 T17238 T17235 arg3Of ],[
R13330 T17239 T17243 arg1Of ",",","
R13331 T17240 T17241 arg1Of 150,mM
R13332 T17242 T17239 arg2Of NaCl,","
R13333 T17242 T17240 arg1Of NaCl,150
R13334 T17243 T17247 arg1Of ",",","
R13335 T17244 T17245 arg1Of 1,mM
R13336 T17246 T17243 arg2Of EDTA,","
R13337 T17246 T17244 arg1Of EDTA,1
R13338 T17247 T17231 arg2Of ",",(
R13339 T17247 T17251 arg1Of ",",","
R13340 T17248 T17249 arg1Of 1,%
R13341 T17250 T17247 arg2Of NP-40,","
R13342 T17250 T17248 arg1Of NP-40,1
R13343 T17252 T17253 arg1Of 0.1,%
R13344 T17254 T17251 arg2Of SDS,","
R13345 T17254 T17252 arg1Of SDS,0.1
R13346 T17254 T17255 arg1Of SDS,","
R13347 T17256 T17257 arg1Of 0.5,%
R13348 T17258 T17255 arg2Of deoxycholate,","
R13349 T17258 T17256 arg1Of deoxycholate,0.5
R13350 T17259 T17231 arg3Of ),(
R13351 T17260 T17292 arg1Of ",",and
R13352 T17261 T17260 arg2Of 1×,","
R13353 T17261 T17262 arg1Of 1×,with
R13354 T17265 T17262 arg2Of buffer,with
R13355 T17265 T17263 arg1Of buffer,high
R13356 T17265 T17264 arg1Of buffer,salt
R13357 T17265 T17266 arg1Of buffer,(
R13358 T17267 T17268 arg1Of 50,mM
R13359 T17269 T17267 arg1Of Tris,50
R13360 T17269 T17270 arg1Of Tris,[
R13361 T17269 T17274 arg1Of Tris,","
R13362 T17271 T17270 arg2Of pH,[
R13363 T17271 T17272 arg1Of pH,8
R13364 T17273 T17270 arg3Of ],[
R13365 T17274 T17278 arg1Of ",",","
R13366 T17275 T17276 arg1Of 500,mM
R13367 T17277 T17274 arg2Of NaCl,","
R13368 T17277 T17275 arg1Of NaCl,500
R13369 T17278 T17282 arg1Of ",",","
R13370 T17279 T17280 arg1Of 1,mM
R13371 T17281 T17278 arg2Of EDTA,","
R13372 T17281 T17279 arg1Of EDTA,1
R13373 T17282 T17266 arg2Of ",",(
R13374 T17282 T17286 arg1Of ",",","
R13375 T17283 T17284 arg1Of 1,%
R13376 T17285 T17282 arg2Of NP-40,","
R13377 T17285 T17283 arg1Of NP-40,1
R13378 T17287 T17288 arg1Of 0.1,%
R13379 T17289 T17286 arg2Of SDS,","
R13380 T17289 T17287 arg1Of SDS,0.1
R13381 T17290 T17266 arg3Of ),(
R13382 T17292 T17226 arg3Of and,washed
R13383 T17292 T17291 arg1Of and,","
R13384 T17293 T17292 arg2Of 1×,and
R13385 T17293 T17294 arg1Of 1×,with
R13386 T17296 T17294 arg2Of buffer,with
R13387 T17296 T17295 arg1Of buffer,TE
R13388 T17300 T17297 arg2Of wash,After
R13389 T17300 T17298 arg1Of wash,the
R13390 T17300 T17299 arg1Of wash,last
R13391 T17302 T17303 arg1Of DNA,was
R13392 T17302 T17304 arg2Of DNA,eluted
R13393 T17304 T17297 arg1Of eluted,After
R13394 T17304 T17301 arg1Of eluted,","
R13395 T17304 T17303 arg2Of eluted,was
R13396 T17304 T17305 arg1Of eluted,by
R13397 T17306 T17305 arg2Of resuspending,by
R13398 T17308 T17306 arg2Of beads,resuspending
R13399 T17308 T17307 arg1Of beads,the
R13400 T17308 T17309 arg1Of beads,in
R13401 T17311 T17309 arg2Of buffer,in
R13402 T17311 T17310 arg1Of buffer,elution
R13403 T17311 T17312 arg1Of buffer,(
R13404 T17313 T17314 arg1Of 1,%
R13405 T17315 T17312 arg2Of SDS,(
R13406 T17315 T17313 arg1Of SDS,1
R13407 T17315 T17316 arg1Of SDS,","
R13408 T17317 T17318 arg1Of 100,mM
R13409 T17319 T17316 arg2Of NaHCO3,","
R13410 T17319 T17317 arg1Of NaHCO3,100
R13411 T17320 T17312 arg3Of ),(
R13412 T17322 T17323 arg1Of input,and
R13413 T17323 T17321 arg1Of and,Both
R13414 T17323 T17326 arg1Of and,were
R13415 T17323 T17328 arg2Of and,treated
R13416 T17325 T17323 arg2Of chromatin,and
R13417 T17325 T17324 arg1Of chromatin,ChIP
R13418 T17328 T17326 arg2Of treated,were
R13419 T17328 T17327 arg1Of treated,then
R13420 T17328 T17329 arg1Of treated,with
R13421 T17328 T17337 arg1Of treated,for
R13422 T17328 T17342 arg1Of treated,","
R13423 T17328 T17343 modOf treated,followed
R13424 T17331 T17329 arg2Of A,with
R13425 T17331 T17330 arg1Of A,RNase
R13426 T17331 T17332 arg1Of A,(
R13427 T17335 T17332 arg2Of total,(
R13428 T17335 T17333 arg1Of total,5
R13429 T17335 T17334 arg1Of total,μg
R13430 T17336 T17332 arg3Of ),(
R13431 T17339 T17337 arg2Of h,for
R13432 T17339 T17338 arg1Of h,1
R13433 T17339 T17340 arg1Of h,at
R13434 T17341 T17340 arg2Of 37°C,at
R13435 T17346 T17343 arg1Of addition,followed
R13436 T17346 T17344 arg2Of addition,by
R13437 T17346 T17345 arg1Of addition,the
R13438 T17346 T17347 arg1Of addition,of
R13439 T17349 T17348 arg1Of K,proteinase
R13440 T17349 T17350 arg1Of K,(
R13441 T17349 T17355 arg1Of K,and
R13442 T17353 T17350 arg2Of total,(
R13443 T17353 T17351 arg1Of total,100
R13444 T17353 T17352 arg1Of total,μg
R13445 T17354 T17350 arg3Of ),(
R13446 T17355 T17347 arg2Of and,of
R13447 T17357 T17355 arg2Of incubation,and
R13448 T17357 T17356 arg1Of incubation,overnight
R13449 T17357 T17358 arg1Of incubation,at
R13450 T17357 T17360 modOf incubation,to
R13451 T17359 T17358 arg2Of 65°C,at
R13452 T17361 T17360 arg1Of reverse,to
R13453 T17362 T17361 arg2Of cross-linking,reverse
R13454 T17363 T17364 arg1Of DNA,was
R13455 T17363 T17366 arg2Of DNA,purified
R13456 T17363 T17384 arg1Of DNA,resuspended
R13457 T17366 T17367 arg1Of purified,with
R13458 T17366 T17377 arg1Of purified,according
R13459 T17366 T17383 arg1Of purified,and
R13460 T17369 T17367 arg2Of columns,with
R13461 T17369 T17368 arg1Of columns,QIAquick
R13462 T17369 T17370 arg1Of columns,(
R13463 T17373 T17370 arg2Of Kit,(
R13464 T17373 T17371 arg1Of Kit,Gel
R13465 T17373 T17372 arg1Of Kit,Extraction
R13466 T17373 T17374 arg1Of Kit,;
R13467 T17375 T17374 arg2Of QIAGEN,;
R13468 T17376 T17370 arg3Of ),(
R13469 T17378 T17377 arg2Of to,according
R13470 T17380 T17379 arg1Of manufacturer,the
R13471 T17380 T17381 arg2Of manufacturer,'s
R13472 T17382 T17378 arg2Of instructions,to
R13473 T17382 T17381 arg1Of instructions,'s
R13474 T17383 T17364 arg2Of and,was
R13475 T17383 T17365 arg1Of and,then
R13476 T17384 T17383 arg2Of resuspended,and
R13477 T17384 T17385 arg1Of resuspended,in
R13478 T17388 T17385 arg2Of volume,in
R13479 T17388 T17386 arg1Of volume,a
R13480 T17388 T17387 arg1Of volume,50-μl
R13481 T17392 T17389 arg2Of detection,For
R13482 T17392 T17390 arg1Of detection,real-time
R13483 T17392 T17391 arg1Of detection,PCR
R13484 T17392 T17393 arg1Of detection,of
R13485 T17395 T17393 arg2Of targets,of
R13486 T17395 T17394 arg1Of targets,immunoprecipitated
R13487 T17395 T17396 arg1Of targets,using
R13488 T17399 T17398 arg1Of Green,SYBR
R13489 T17401 T17396 arg2Of Kit,using
R13490 T17401 T17397 arg1Of Kit,the
R13491 T17401 T17399 arg1Of Kit,Green
R13492 T17401 T17400 arg1Of Kit,PCR
R13493 T17405 T17403 arg1Of curve,a
R13494 T17405 T17404 arg1Of curve,standard
R13495 T17405 T17406 arg1Of curve,was
R13496 T17405 T17407 arg2Of curve,obtained
R13497 T17407 T17406 arg2Of obtained,was
R13498 T17407 T17408 arg1Of obtained,with
R13499 T17407 T17418 arg1Of obtained,and
R13500 T17410 T17408 arg2Of dilutions,with
R13501 T17410 T17409 arg1Of dilutions,serial
R13502 T17410 T17411 arg1Of dilutions,of
R13503 T17413 T17411 arg2Of DNA,of
R13504 T17413 T17412 arg1Of DNA,input
R13505 T17413 T17414 arg1Of DNA,for
R13506 T17416 T17414 arg2Of sample,for
R13507 T17416 T17415 arg1Of sample,each
R13508 T17418 T17389 arg1Of and,For
R13509 T17418 T17402 arg1Of and,","
R13510 T17418 T17417 arg1Of and,","
R13511 T17422 T17419 arg1Of DNA,1
R13512 T17422 T17420 arg1Of DNA,μl
R13513 T17422 T17421 arg1Of DNA,ChIP
R13514 T17422 T17423 arg1Of DNA,was
R13515 T17422 T17424 arg2Of DNA,used
R13516 T17424 T17418 arg2Of used,and
R13517 T17424 T17423 arg2Of used,was
R13518 T17424 T17425 arg1Of used,per
R13519 T17427 T17425 arg2Of reaction,per
R13520 T17427 T17426 arg1Of reaction,PCR
R13521 T17427 T17428 arg1Of reaction,(
R13522 T17429 T17428 arg2Of performed,(
R13523 T17429 T17430 arg1Of performed,in
R13524 T17431 T17430 arg2Of duplicates,in
R13525 T17432 T17428 arg3Of ),(
R13526 T17434 T17433 arg1Of curves,Melt
R13527 T17434 T17435 arg1Of curves,and
R13528 T17435 T17438 arg1Of and,were
R13529 T17435 T17439 arg2Of and,analyzed
R13530 T17437 T17435 arg2Of gels,and
R13531 T17437 T17436 arg1Of gels,agarose
R13532 T17439 T17438 arg2Of analyzed,were
R13533 T17439 T17440 modOf analyzed,to
R13534 T17441 T17440 arg1Of ensure,to
R13535 T17442 T17441 arg2Of amplification,ensure
R13536 T17442 T17443 arg1Of amplification,of
R13537 T17446 T17443 arg2Of sequences,of
R13538 T17446 T17444 arg1Of sequences,specific
R13539 T17446 T17445 arg1Of sequences,target
R13540 T17447 T17458 arg1Of Refer,for
R13541 T17450 T17447 arg2Of S1,Refer
R13542 T17450 T17448 arg1Of S1,to
R13543 T17450 T17449 arg1Of S1,Table
R13544 T17450 T17451 arg1Of S1,(
R13545 T17452 T17451 arg2Of available,(
R13546 T17452 T17453 arg1Of available,at
R13547 T17454 T17453 arg2Of http,at
R13548 T17454 T17455 arg1Of http,:
R13549 T17456 T17455 arg2Of //www.jem.org/cgi/content/full/jem.20081242/DC1,:
R13550 T17457 T17451 arg3Of ),(
R13551 T17460 T17458 arg2Of list,for
R13552 T17460 T17459 arg1Of list,a
R13553 T17460 T17461 arg1Of list,of
R13554 T17463 T17461 arg2Of sets,of
R13555 T17463 T17462 arg1Of sets,primer
R13556 T17465 T17464 arg1Of data,The
R13557 T17465 T17466 arg1Of data,are
R13558 T17465 T17467 arg2Of data,presented
R13559 T17467 T17466 arg2Of presented,are
R13560 T17467 T17468 arg1Of presented,as
R13561 T17467 T17479 arg1Of presented,","
R13562 T17467 T17480 modOf presented,assuming
R13563 T17470 T17468 arg2Of number,as
R13564 T17470 T17469 arg1Of number,the
R13565 T17470 T17471 arg1Of number,of
R13566 T17474 T17471 arg2Of sequences,of
R13567 T17474 T17472 arg2Of sequences,immunoprecipitated
R13568 T17474 T17473 arg1Of sequences,target
R13569 T17474 T17475 arg1Of sequences,relative
R13570 T17475 T17476 arg1Of relative,to
R13571 T17478 T17476 arg2Of chromatin,to
R13572 T17478 T17477 arg1Of chromatin,input
R13573 T17482 T17480 arg2Of copies,assuming
R13574 T17482 T17481 arg1Of copies,two
R13575 T17482 T17483 arg1Of copies,of
R13576 T17482 T17486 arg1Of copies,per
R13577 T17485 T17483 arg2Of sequence,of
R13578 T17485 T17484 arg1Of sequence,target
R13579 T17487 T17486 arg2Of cell,per
R13580 T17487 T17488 arg1Of cell,equivalent
R13581 T17487 T17489 arg2Of cell,used
R13582 T17489 T17490 arg1Of used,for
R13583 T17492 T17490 arg2Of ChIP,for
R13584 T17492 T17491 arg1Of ChIP,the

GO-BP

Id Subject Object Predicate Lexical cue
T16944 276-278 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T16945 632-637 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T16946 743-754 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T16947 1681-1691 http://purl.obolibrary.org/obo/GO_0004175 denotes proteinase

GO-MF

Id Subject Object Predicate Lexical cue
T17518 276-278 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T17519 743-754 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T17520 1005-1013 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T17521 1681-1691 http://purl.obolibrary.org/obo/GO_0004175 denotes proteinase

GO-CC

Id Subject Object Predicate Lexical cue
T17522 49-54 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17523 345-350 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17524 615-620 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17525 1036-1040 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T17526 2520-2524 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T17527 772-781 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T17528 874-883 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T17529 940-949 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T17530 1582-1591 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T17531 2466-2475 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T17532 1005-1013 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T17533 1005-1013 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

sentences

Id Subject Object Predicate Lexical cue
T16948 0-32 Sentence denotes ChIP and real-time PCR analysis.
T16949 33-279 Sentence denotes 20 × 106 CD8+ T cells per immunoprecipitation were fixed by adding a 1/10th volume of fixation solution (11.1% formaldehyde, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 50 mM Hepes) to 1 volume of culture media and were incubated for 10 or 30 min at RT.
T16950 280-338 Sentence denotes Fixation was stopped with 120 mM glycine on ice for 5 min.
T16951 339-558 Sentence denotes Fixed cells were washed 2× with cold PBS, 1× with cold solution I (10 mM Tris [pH 7.5], 10 mM EDTA, 0.5 mM EGTA, 1% Triton X-100), and 1× with cold solution II (10 mM Tris [pH 7.5], 1 mM EDTA, 0.5 mM EGTA, 200 mM NaCl).
T16952 559-843 Sentence denotes After washes, cell pellets were resuspended at 40 × 106 cells/ml in ChIP lysis buffer (150 mM NaCl, 25 mM Tris [pH 7.5], 1% Triton X-100, 0.1% SDS, 0.5% deoxycholate plus protease and phosphatase inhibitors), and chromatin was sheared with a sonicator to yield 0.5–1-kb DNA fragments.
T16953 844-1053 Sentence denotes After preclearing the sheared chromatin with protein A–sepharose beads and removing 5% as input chromatin, immunoprecipitation was performed by adding optimized antibody amounts (per 20 × 106 cell equivalents:
T16954 1054-1221 Sentence denotes 2.5 μg anti-Eomes, 1:100 dilution anti-Runx3), followed by overnight incubation at 4°C; protein A–sepharose beads were added for the last 3 h of the incubation period.
T16955 1222-1456 Sentence denotes Beads were washed 2× with RIPA buffer (50 mM Tris [pH 8], 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% deoxycholate), 1× with high salt buffer (50 mM Tris [pH 8], 500 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS), and 1× with TE buffer.
T16956 1457-1561 Sentence denotes After the last wash, DNA was eluted by resuspending the beads in elution buffer (1% SDS, 100 mM NaHCO3).
T16957 1562-1767 Sentence denotes Both input and ChIP chromatin were then treated with RNase A (5 μg total) for 1 h at 37°C, followed by the addition of proteinase K (100 μg total) and overnight incubation at 65°C to reverse cross-linking.
T16958 1768-1920 Sentence denotes DNA was then purified with QIAquick columns (Gel Extraction Kit; QIAGEN) according to the manufacturer's instructions and resuspended in a 50-μl volume.
T16959 1921-2161 Sentence denotes For real-time PCR detection of immunoprecipitated targets using the SYBR Green PCR Kit, a standard curve was obtained with serial dilutions of input DNA for each sample, and 1 μl ChIP DNA was used per PCR reaction (performed in duplicates).
T16960 2162-2258 Sentence denotes Melt curves and agarose gels were analyzed to ensure amplification of specific target sequences.
T16961 2259-2371 Sentence denotes Refer to Table S1 (available at http://www.jem.org/cgi/content/full/jem.20081242/DC1) for a list of primer sets.
T16962 2372-2554 Sentence denotes The data are presented as the number of immunoprecipitated target sequences relative to input chromatin, assuming two copies of target sequence per cell equivalent used for the ChIP.
T188 0-32 Sentence denotes ChIP and real-time PCR analysis.
T189 33-279 Sentence denotes 20 × 106 CD8+ T cells per immunoprecipitation were fixed by adding a 1/10th volume of fixation solution (11.1% formaldehyde, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 50 mM Hepes) to 1 volume of culture media and were incubated for 10 or 30 min at RT.
T190 280-338 Sentence denotes Fixation was stopped with 120 mM glycine on ice for 5 min.
T191 339-558 Sentence denotes Fixed cells were washed 2× with cold PBS, 1× with cold solution I (10 mM Tris [pH 7.5], 10 mM EDTA, 0.5 mM EGTA, 1% Triton X-100), and 1× with cold solution II (10 mM Tris [pH 7.5], 1 mM EDTA, 0.5 mM EGTA, 200 mM NaCl).
T192 559-843 Sentence denotes After washes, cell pellets were resuspended at 40 × 106 cells/ml in ChIP lysis buffer (150 mM NaCl, 25 mM Tris [pH 7.5], 1% Triton X-100, 0.1% SDS, 0.5% deoxycholate plus protease and phosphatase inhibitors), and chromatin was sheared with a sonicator to yield 0.5–1-kb DNA fragments.
T193 844-1053 Sentence denotes After preclearing the sheared chromatin with protein A–sepharose beads and removing 5% as input chromatin, immunoprecipitation was performed by adding optimized antibody amounts (per 20 × 106 cell equivalents:
T194 1054-1221 Sentence denotes 2.5 μg anti-Eomes, 1:100 dilution anti-Runx3), followed by overnight incubation at 4°C; protein A–sepharose beads were added for the last 3 h of the incubation period.
T195 1222-1456 Sentence denotes Beads were washed 2× with RIPA buffer (50 mM Tris [pH 8], 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% deoxycholate), 1× with high salt buffer (50 mM Tris [pH 8], 500 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS), and 1× with TE buffer.
T196 1457-1561 Sentence denotes After the last wash, DNA was eluted by resuspending the beads in elution buffer (1% SDS, 100 mM NaHCO3).
T197 1562-1767 Sentence denotes Both input and ChIP chromatin were then treated with RNase A (5 μg total) for 1 h at 37°C, followed by the addition of proteinase K (100 μg total) and overnight incubation at 65°C to reverse cross-linking.
T198 1768-1920 Sentence denotes DNA was then purified with QIAquick columns (Gel Extraction Kit; QIAGEN) according to the manufacturer's instructions and resuspended in a 50-μl volume.
T199 1921-2161 Sentence denotes For real-time PCR detection of immunoprecipitated targets using the SYBR Green PCR Kit, a standard curve was obtained with serial dilutions of input DNA for each sample, and 1 μl ChIP DNA was used per PCR reaction (performed in duplicates).
T200 2162-2258 Sentence denotes Melt curves and agarose gels were analyzed to ensure amplification of specific target sequences.
T201 2259-2371 Sentence denotes Refer to Table S1 (available at http://www.jem.org/cgi/content/full/jem.20081242/DC1) for a list of primer sets.
T202 2372-2554 Sentence denotes The data are presented as the number of immunoprecipitated target sequences relative to input chromatin, assuming two copies of target sequence per cell equivalent used for the ChIP.

events-check-again

Id Subject Object Predicate Lexical cue
T17549 42-45 Protein denotes CD8
T17550 1066-1071 Protein denotes Eomes
T17551 1093-1098 Protein denotes Runx3

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T17552 725-754 Protein denotes plus protease and phosphatase
T17553 1615-1622 Protein denotes RNase A
T17554 2306-2309 Protein denotes org
T17555 2310-2313 Protein denotes cgi

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T16941 42-45 Protein denotes CD8
T16942 1066-1071 Protein denotes Eomes
T16943 1093-1098 Protein denotes Runx3

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T17493 42-45 P10966 denotes CD8
T17494 42-45 P01732 denotes CD8
T17495 418-420 P0A7Z4 denotes pH
T17496 512-514 P0A7Z4 denotes pH
T17497 671-673 P0A7Z4 denotes pH
T17498 1066-1071 O95936 denotes Eomes
T17499 1093-1098 Q13761 denotes Runx3
T17500 1273-1275 P0A7Z4 denotes pH
T17501 1382-1384 P0A7Z4 denotes pH
T17502 2448-2456 Q04864 denotes relative

test2

Id Subject Object Predicate Lexical cue
T16938 42-45 Protein denotes CD8
T16939 1066-1071 Protein denotes Eomes
T16940 1093-1098 Protein denotes Runx3