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PMC:2626671 / 29961-30351 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T16555 0-2 TO denotes To
T16556 3-9 VB denotes detect
T16557 10-20 NN denotes expression
T16558 21-23 IN denotes of
T16559 24-31 NN denotes surface
T16560 32-41 NN denotes molecules
T16561 41-42 -COMMA- denotes ,
T16562 43-48 NN denotes cells
T16563 49-53 VB denotes were
T16564 54-60 VB denotes washed
T16565 61-63 IN denotes in
T16566 64-67 NNP denotes PBS
T16567 67-68 -COMMA- denotes ,
T16568 69-80 VB denotes resuspended
T16569 81-83 IN denotes in
T16570 84-88 NN denotes FACS
T16571 89-93 NN denotes wash
T16572 94-100 NN denotes buffer
T16573 101-102 -LRB- denotes (
T16574 102-103 CD denotes 3
T16575 103-104 NN denotes %
T16576 105-108 NN denotes FBS
T16577 108-109 -COMMA- denotes ,
T16578 110-113 CD denotes 0.1
T16579 113-114 NN denotes %
T16580 115-121 NN denotes sodium
T16581 122-127 NN denotes azide
T16582 127-128 -COMMA- denotes ,
T16583 129-131 CD denotes 30
T16584 132-134 NN denotes mM
T16585 135-140 NNP denotes Hepes
T16586 140-141 -COMMA- denotes ,
T16587 142-144 CD denotes 1×
T16588 145-148 NNP denotes PBS
T16589 148-149 -RRB- denotes )
T16590 150-160 VB denotes containing
T16591 161-164 DT denotes the
T16592 165-175 NN denotes antibodies
T16593 176-185 VB denotes indicated
T16594 186-188 IN denotes in
T16595 189-192 DT denotes the
T16596 193-200 NN denotes figures
T16597 201-203 IN denotes at
T16609 282-283 -LRB- denotes (
T16610 283-285 NN denotes RT
T16611 285-286 -RRB- denotes )
T16612 286-287 -COMMA- denotes ,
T16613 288-294 VB denotes washed
T16614 294-295 -COMMA- denotes ,
T16615 296-299 CC denotes and
T16616 300-311 VB denotes resuspended
T16617 312-314 IN denotes in
T16618 315-316 CD denotes 2
T16619 316-317 NN denotes %
T16620 318-330 NN denotes formaldehyde
T16621 331-339 JJ denotes fixative
T16622 340-348 NN denotes solution
T16623 349-355 IN denotes before
T16624 356-367 NN denotes acquisition
T16625 368-370 IN denotes on
T16626 371-372 DT denotes a
T16627 373-384 NN denotes FACSCalibur
T16628 385-386 -LRB- denotes (
T16629 386-388 NN denotes BD
T16630 388-389 -RRB- denotes )
T16598 204-214 RB denotes previously
T16599 215-224 VB denotes optimized
T16600 225-239 NN denotes concentrations
T16601 239-240 -COMMA- denotes ,
T16602 241-250 VB denotes incubated
T16603 251-254 IN denotes for
T16604 255-257 CD denotes 15
T16605 258-261 NN denotes min
T16606 262-264 IN denotes at
T16607 265-269 NN denotes room
T16608 270-281 NN denotes temperature
R12718 T16556 T16555 arg1Of detect,To
R12719 T16557 T16556 arg2Of expression,detect
R12720 T16557 T16558 arg1Of expression,of
R12721 T16560 T16558 arg2Of molecules,of
R12722 T16560 T16559 arg1Of molecules,surface
R12723 T16562 T16556 arg1Of cells,detect
R12724 T16562 T16563 arg1Of cells,were
R12725 T16562 T16564 arg2Of cells,washed
R12726 T16562 T16568 arg1Of cells,resuspended
R12727 T16562 T16602 arg2Of cells,incubated
R12728 T16562 T16613 arg1Of cells,washed
R12729 T16562 T16616 arg1Of cells,resuspended
R12730 T16564 T16563 arg2Of washed,were
R12731 T16564 T16565 arg1Of washed,in
R12732 T16564 T16567 arg1Of washed,","
R12733 T16566 T16565 arg2Of PBS,in
R12734 T16567 T16602 modOf ",",incubated
R12735 T16567 T16612 arg1Of ",",","
R12736 T16568 T16567 arg2Of resuspended,","
R12737 T16568 T16569 arg1Of resuspended,in
R12738 T16572 T16570 arg1Of buffer,FACS
R12739 T16572 T16571 arg1Of buffer,wash
R12740 T16572 T16573 arg1Of buffer,(
R12741 T16572 T16590 arg1Of buffer,containing
R12742 T16572 T16593 arg1Of buffer,indicated
R12743 T16574 T16575 arg1Of 3,%
R12744 T16576 T16573 arg2Of FBS,(
R12745 T16576 T16574 arg1Of FBS,3
R12746 T16576 T16577 arg1Of FBS,","
R12747 T16576 T16582 arg1Of FBS,","
R12748 T16576 T16586 arg1Of FBS,","
R12749 T16578 T16579 arg1Of 0.1,%
R12750 T16581 T16577 arg2Of azide,","
R12751 T16581 T16578 arg1Of azide,0.1
R12752 T16581 T16580 arg1Of azide,sodium
R12753 T16584 T16583 arg1Of mM,30
R12754 T16585 T16582 arg2Of Hepes,","
R12755 T16585 T16584 arg1Of Hepes,mM
R12756 T16588 T16586 arg2Of PBS,","
R12757 T16588 T16587 arg1Of PBS,1×
R12758 T16589 T16573 arg3Of ),(
R12759 T16592 T16590 arg2Of antibodies,containing
R12760 T16592 T16591 arg1Of antibodies,the
R12761 T16593 T16568 arg2Of indicated,resuspended
R12762 T16593 T16594 arg1Of indicated,in
R12763 T16593 T16597 arg1Of indicated,at
R12764 T16593 T16601 arg1Of indicated,","
R12765 T16596 T16594 arg2Of figures,in
R12766 T16596 T16595 arg1Of figures,the
R12767 T16599 T16598 arg1Of optimized,previously
R12768 T16600 T16597 arg2Of concentrations,at
R12769 T16600 T16599 arg1Of concentrations,optimized
R12770 T16602 T16603 arg1Of incubated,for
R12771 T16605 T16603 arg2Of min,for
R12772 T16605 T16604 arg1Of min,15
R12773 T16605 T16606 arg1Of min,at
R12774 T16608 T16606 arg2Of temperature,at
R12775 T16608 T16607 arg1Of temperature,room
R12776 T16608 T16609 arg1Of temperature,(
R12777 T16610 T16609 arg2Of RT,(
R12778 T16611 T16609 arg3Of ),(
R12779 T16612 T16615 arg1Of ",",and
R12780 T16613 T16612 arg2Of washed,","
R12781 T16615 T16555 modOf and,To
R12782 T16615 T16561 arg1Of and,","
R12783 T16615 T16614 arg1Of and,","
R12784 T16616 T16615 arg2Of resuspended,and
R12785 T16616 T16617 arg1Of resuspended,in
R12786 T16616 T16623 arg1Of resuspended,before
R12787 T16616 T16625 arg1Of resuspended,on
R12788 T16618 T16619 arg1Of 2,%
R12789 T16622 T16617 arg2Of solution,in
R12790 T16622 T16618 arg1Of solution,2
R12791 T16622 T16620 arg1Of solution,formaldehyde
R12792 T16622 T16621 arg1Of solution,fixative
R12793 T16624 T16623 arg2Of acquisition,before
R12794 T16627 T16625 arg2Of FACSCalibur,on
R12795 T16627 T16626 arg1Of FACSCalibur,a
R12796 T16627 T16628 arg1Of FACSCalibur,(
R12797 T16629 T16628 arg2Of BD,(
R12798 T16630 T16628 arg3Of ),(

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T16432 0-2 TO denotes To
T16433 3-9 VB denotes detect
T16434 10-20 NN denotes expression
T16435 21-23 IN denotes of
T16436 24-31 NN denotes surface
T16437 32-41 NNS denotes molecules
T16438 41-42 , denotes ,
T16439 43-48 NNS denotes cells
T16440 49-53 VBD denotes were
T16441 54-60 VBN denotes washed
T16442 61-63 IN denotes in
T16443 64-67 NNP denotes PBS
T16444 67-68 , denotes ,
T16445 69-80 VBD denotes resuspended
T16446 81-83 IN denotes in
T16447 84-88 NNP denotes FACS
T16448 89-93 VBP denotes wash
T16449 94-100 NN denotes buffer
T16450 101-102 -LRB- denotes (
T16451 102-103 CD denotes 3
T16452 103-104 NN denotes %
T16453 105-108 NNP denotes FBS
T16454 108-109 , denotes ,
T16455 110-113 CD denotes 0.1
T16456 113-114 NN denotes %
T16457 115-121 NN denotes sodium
T16458 122-127 NN denotes azide
T16459 127-128 , denotes ,
T16460 129-131 CD denotes 30
T16461 132-134 NNP denotes mM
T16462 135-140 NNP denotes Hepes
T16463 140-141 , denotes ,
T16464 142-143 CD denotes 1
T16465 143-144 CD denotes ×
T16466 145-148 NNP denotes PBS
T16467 148-149 -RRB- denotes )
T16468 150-160 VBG denotes containing
T16469 161-164 DT denotes the
T16470 165-175 NNS denotes antibodies
T16471 176-185 VBD denotes indicated
T16472 186-188 IN denotes in
T16473 189-192 DT denotes the
T16474 193-200 NNS denotes figures
T16475 201-203 IN denotes at
T16476 204-214 RB denotes previously
T16477 215-224 VBN denotes optimized
T16478 225-239 NNS denotes concentrations
T16479 239-240 , denotes ,
T16480 241-250 VBN denotes incubated
T16481 251-254 IN denotes for
T16482 255-257 CD denotes 15
T16483 258-261 NN denotes min
T16484 262-264 IN denotes at
T16485 265-269 NN denotes room
T16486 270-281 NN denotes temperature
T16487 282-283 -LRB- denotes (
T16488 283-285 NNP denotes RT
T16489 285-286 -RRB- denotes )
T16490 286-287 , denotes ,
T16491 288-294 VBD denotes washed
T16492 294-295 , denotes ,
T16493 296-299 CC denotes and
T16494 300-311 VBD denotes resuspended
T16495 312-314 IN denotes in
T16496 315-316 CD denotes 2
T16497 316-317 NN denotes %
T16498 318-330 NN denotes formaldehyde
T16499 331-339 JJ denotes fixative
T16500 340-348 NN denotes solution
T16501 349-355 IN denotes before
T16502 356-367 NN denotes acquisition
T16503 368-370 IN denotes on
T16504 371-372 DT denotes a
T16505 373-384 NNP denotes FACSCalibur
T16506 385-386 -LRB- denotes (
T16507 386-388 NNP denotes BD
T16508 388-389 -RRB- denotes )
T16509 389-390 . denotes .
R12592 T16432 T16433 aux To,detect
R12593 T16433 T16441 advcl detect,washed
R12594 T16434 T16433 dobj expression,detect
R12595 T16435 T16434 prep of,expression
R12596 T16436 T16437 compound surface,molecules
R12597 T16437 T16435 pobj molecules,of
R12598 T16438 T16441 punct ",",washed
R12599 T16439 T16441 nsubjpass cells,washed
R12600 T16440 T16441 auxpass were,washed
R12601 T16441 T16441 ROOT washed,washed
R12602 T16442 T16441 prep in,washed
R12603 T16443 T16442 pobj PBS,in
R12604 T16444 T16441 punct ",",washed
R12605 T16445 T16441 conj resuspended,washed
R12606 T16446 T16448 mark in,wash
R12607 T16447 T16448 nsubj FACS,wash
R12608 T16448 T16471 meta wash,indicated
R12609 T16449 T16448 dobj buffer,wash
R12610 T16450 T16453 punct (,FBS
R12611 T16451 T16452 nummod 3,%
R12612 T16452 T16453 npadvmod %,FBS
R12613 T16453 T16471 nsubj FBS,indicated
R12614 T16454 T16453 punct ",",FBS
R12615 T16455 T16456 nummod 0.1,%
R12616 T16456 T16458 compound %,azide
R12617 T16457 T16458 compound sodium,azide
R12618 T16458 T16453 appos azide,FBS
R12619 T16459 T16458 punct ",",azide
R12620 T16460 T16462 nummod 30,Hepes
R12621 T16461 T16462 compound mM,Hepes
R12622 T16462 T16458 appos Hepes,azide
R12623 T16463 T16462 punct ",",Hepes
R12624 T16464 T16466 nummod 1,PBS
R12625 T16465 T16466 nummod ×,PBS
R12626 T16466 T16462 appos PBS,Hepes
R12627 T16467 T16462 punct ),Hepes
R12628 T16468 T16458 acl containing,azide
R12629 T16469 T16470 det the,antibodies
R12630 T16470 T16468 dobj antibodies,containing
R12631 T16471 T16441 conj indicated,washed
R12632 T16472 T16471 prep in,indicated
R12633 T16473 T16474 det the,figures
R12634 T16474 T16472 pobj figures,in
R12635 T16475 T16471 prep at,indicated
R12636 T16476 T16477 advmod previously,optimized
R12637 T16477 T16478 amod optimized,concentrations
R12638 T16478 T16475 pobj concentrations,at
R12639 T16479 T16471 punct ",",indicated
R12640 T16480 T16491 nsubj incubated,washed
R12641 T16481 T16480 prep for,incubated
R12642 T16482 T16483 nummod 15,min
R12643 T16483 T16481 pobj min,for
R12644 T16484 T16483 prep at,min
R12645 T16485 T16486 compound room,temperature
R12646 T16486 T16484 pobj temperature,at
R12647 T16487 T16488 punct (,RT
R12648 T16488 T16483 parataxis RT,min
R12649 T16489 T16488 punct ),RT
R12650 T16490 T16483 punct ",",min
R12651 T16491 T16471 conj washed,indicated
R12652 T16492 T16491 punct ",",washed
R12653 T16493 T16491 cc and,washed
R12654 T16494 T16491 conj resuspended,washed
R12655 T16495 T16494 prep in,resuspended
R12656 T16496 T16497 nummod 2,%
R12657 T16497 T16500 nmod %,solution
R12658 T16498 T16500 nmod formaldehyde,solution
R12659 T16499 T16500 amod fixative,solution
R12660 T16500 T16495 pobj solution,in
R12661 T16501 T16500 prep before,solution
R12662 T16502 T16501 pobj acquisition,before
R12663 T16503 T16502 prep on,acquisition
R12664 T16504 T16505 det a,FACSCalibur
R12665 T16505 T16503 pobj FACSCalibur,on
R12666 T16506 T16507 punct (,BD
R12667 T16507 T16505 appos BD,FACSCalibur
R12668 T16508 T16505 punct ),FACSCalibur
R12669 T16509 T16441 punct .,washed

GO-BP

Id Subject Object Predicate Lexical cue
T16384 283-285 http://purl.obolibrary.org/obo/GO_0003964 denotes RT

GO-MF

Id Subject Object Predicate Lexical cue
T16634 165-175 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T16635 283-285 http://purl.obolibrary.org/obo/GO_0003964 denotes RT

GO-CC

Id Subject Object Predicate Lexical cue
T16637 43-48 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16639 165-175 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T16640 165-175 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies

sentences

Id Subject Object Predicate Lexical cue
T16375 0-390 Sentence denotes To detect expression of surface molecules, cells were washed in PBS, resuspended in FACS wash buffer (3% FBS, 0.1% sodium azide, 30 mM Hepes, 1× PBS) containing the antibodies indicated in the figures at previously optimized concentrations, incubated for 15 min at room temperature (RT), washed, and resuspended in 2% formaldehyde fixative solution before acquisition on a FACSCalibur (BD).
T181 0-390 Sentence denotes To detect expression of surface molecules, cells were washed in PBS, resuspended in FACS wash buffer (3% FBS, 0.1% sodium azide, 30 mM Hepes, 1× PBS) containing the antibodies indicated in the figures at previously optimized concentrations, incubated for 15 min at room temperature (RT), washed, and resuspended in 2% formaldehyde fixative solution before acquisition on a FACSCalibur (BD).