PMC:2626671 / 25277-35396 JSON TXT

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2_test

Id	Subject	Object	Predicate	Lexical cue
19139168-12464175-59674071	2259-2261	12464175	denotes	12
19139168-12796513-59674072	2263-2265	12796513	denotes	13
19139168-12464175-59674073	2897-2899	12464175	denotes	12
19139168-12796513-59674074	2901-2903	12796513	denotes	13
19139168-15516924-59674075	4679-4681	15516924	denotes	28
19139168-16273099-59674076	5775-5776	16273099	denotes	8
19139168-16510851-59674077	8452-8454	16510851	denotes	29

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T14019	23-33	NN	denotes	Antibodies
T14020	34-37	CC	denotes	and
T14021	38-46	NN	denotes	reagents
T14022	48-51	DT	denotes	The
T14023	52-61	JJ	denotes	following
T14024	62-72	NN	denotes	antibodies
T14025	73-77	VB	denotes	used
T14026	78-81	IN	denotes	for
T14027	82-95	JJ	denotes	intracellular
T14028	96-98	CC	denotes	or
T14029	99-106	NN	denotes	surface
T14030	107-113	NN	denotes	stains
T14031	114-118	VB	denotes	were
T14032	119-127	VB	denotes	obtained
T14033	128-132	IN	denotes	from
T14034	133-144	NN	denotes	eBioscience
T14035	144-145	-COLON-	denotes	:
T14036	146-155	NN	denotes	anti–IL-2
T14037	155-156	-COMMA-	denotes	,
T14038	157-167	JJ	denotes	anti–IFN-γ
T14039	167-168	-COMMA-	denotes	,
T14040	169-177	JJ	denotes	anti-TNF
T14041	177-178	-COMMA-	denotes	,
T14042	179-192	NN	denotes	anti–granzyme
T14043	193-194	NN	denotes	B
T14044	194-195	-COMMA-	denotes	,
T14045	196-204	NN	denotes	anti-CD8
T14046	204-205	-COMMA-	denotes	,
T14047	206-215	NN	denotes	anti-CD25
T14048	215-216	-COMMA-	denotes	,
T14049	217-220	CC	denotes	and
T14050	221-230	NN	denotes	anti-CD44
T14051	232-241	NN	denotes	Anti-CD69
T14052	242-245	VB	denotes	was
T14053	246-255	VB	denotes	purchased
T14054	256-260	IN	denotes	from
T14055	261-263	NN	denotes	BD
T14056	265-268	IN	denotes	For
T14057	269-273	NN	denotes	ChIP
T14058	274-285	NN	denotes	experiments
T14059	285-286	-COMMA-	denotes	,
T14060	287-290	DT	denotes	the
T14061	291-301	JJ	denotes	anti-Eomes
T14062	302-310	NN	denotes	antibody
T14063	311-314	VB	denotes	was
T14064	315-323	VB	denotes	obtained
T14065	324-328	IN	denotes	from
T14066	329-334	NN	denotes	Abcam
T14067	335-338	CC	denotes	and
T14068	339-342	DT	denotes	the
T14069	343-353	JJ	denotes	anti-Runx3
T14070	354-362	NN	denotes	antibody
T14071	363-366	VB	denotes	was
T14072	367-375	VB	denotes	produced
T14073	376-378	IN	denotes	by
T14074	379-382	DT	denotes	the
T14075	383-389	NNP	denotes	Groner
T14076	390-400	NN	denotes	laboratory
T14077	402-405	DT	denotes	The
T14078	406-415	VB	denotes	following
T14079	416-426	NN	denotes	antibodies
T14080	427-431	VB	denotes	were
T14081	432-436	VB	denotes	used
T14082	437-440	IN	denotes	for
T14083	441-455	NN	denotes	immunoblotting
T14084	455-456	-COLON-	denotes	:
T14085	457-469	NN	denotes	antiperforin
T14086	470-471	-LRB-	denotes	(
T14087	471-476	NN	denotes	Abcam
T14088	476-477	-RRB-	denotes	)
T14089	477-478	-COMMA-	denotes	,
T14090	479-489	JJ	denotes	anti-Eomes
T14091	490-491	-LRB-	denotes	(
T14092	491-496	NN	denotes	Abcam
T14093	496-497	-RRB-	denotes	)
T14094	497-498	-COMMA-	denotes	,
T14095	499-502	CC	denotes	and
T14096	503-514	NN	denotes	anti–Pol-II
T14097	515-516	-LRB-	denotes	(
T14098	516-521	NNP	denotes	Santa
T14099	522-526	NNP	denotes	Cruz
T14100	527-540	NNP	denotes	Biotechnology
T14101	540-541	-COMMA-	denotes	,
T14102	542-546	NNP	denotes	Inc.
T14103	546-547	-RRB-	denotes	)
T14104	549-552	DT	denotes	The
T14105	553-558	JJ	denotes	T-bet
T14106	559-567	NN	denotes	antibody
T14107	568-571	VB	denotes	was
T14108	572-580	VB	denotes	provided
T14109	581-583	IN	denotes	by
T14110	584-586	NNP	denotes	L.
T14111	587-595	NNP	denotes	Glimcher
T14112	596-597	-LRB-	denotes	(
T14113	597-604	NNP	denotes	Harvard
T14114	605-611	NNP	denotes	School
T14115	612-614	IN	denotes	of
T14116	615-621	NNP	denotes	Public
T14117	622-628	NNP	denotes	Health
T14118	628-629	-COMMA-	denotes	,
T14119	630-636	NNP	denotes	Boston
T14120	636-637	-COMMA-	denotes	,
T14121	638-640	NNP	denotes	MA
T14122	640-641	-RRB-	denotes	)
T14123	643-646	DT	denotes	The
T14124	647-656	VB	denotes	following
T14125	657-665	NN	denotes	reagents
T14126	666-670	VB	denotes	were
T14127	671-675	VB	denotes	used
T14128	676-679	IN	denotes	for
T14129	680-683	DT	denotes	the
T14130	684-695	NN	denotes	experiments
T14131	696-705	VB	denotes	presented
T14132	706-708	IN	denotes	in
T14133	709-713	DT	denotes	this
T14134	714-720	NN	denotes	report
T14135	720-721	-COLON-	denotes	:
T14136	722-729	NN	denotes	Annexin
T14137	730-736	NNP	denotes	V–FITC
T14138	737-746	NNP	denotes	Apoptosis
T14139	747-756	NN	denotes	Detection
T14140	757-760	NN	denotes	Kit
T14141	761-762	-LRB-	denotes	(
T14142	762-764	NN	denotes	BD
T14143	764-765	-RRB-	denotes	)
T14144	765-766	-COMMA-	denotes	,
T14145	767-770	NN	denotes	CD8
T14146	771-779	JJ	denotes	Negative
T14147	780-789	NN	denotes	Isolation
T14148	790-793	NN	denotes	Kit
T14149	794-795	-LRB-	denotes	(
T14150	795-805	NN	denotes	Invitrogen
T14151	805-806	-RRB-	denotes	)
T14152	806-807	-COMMA-	denotes	,
T14153	808-811	NN	denotes	CD8
T14154	812-822	NN	denotes	MicroBeads
T14155	823-824	-LRB-	denotes	(
T14156	824-832	NNP	denotes	Miltenyi
T14157	833-839	NNP	denotes	Biotec
T14158	839-840	-RRB-	denotes	)
T14159	840-841	-COMMA-	denotes	,
T14160	842-845	CC	denotes	and
T14161	846-850	NNP	denotes	SYBR
T14162	851-856	NNP	denotes	Green
T14163	857-860	NN	denotes	PCR
T14164	861-865	NNP	denotes	Core
T14165	866-874	NNP	denotes	Reagents
T14166	875-876	-LRB-	denotes	(
T14167	876-883	NNP	denotes	Applied
T14168	884-894	NNP	denotes	Biosystems
T14169	894-895	-RRB-	denotes	)
T14170	897-900	DT	denotes	The
T14171	901-905	NN	denotes	Gp33
T14172	906-913	NN	denotes	peptide
T14173	914-915	-LRB-	denotes	(
T14174	915-924	NN	denotes	KAVYNFATC
T14175	924-925	-RRB-	denotes	)
T14176	926-929	VB	denotes	was
T14177	930-941	VB	denotes	synthesized
T14178	942-944	IN	denotes	by
T14179	945-948	DT	denotes	the
T14180	949-954	NNP	denotes	Tufts
T14181	955-965	NNP	denotes	University
T14182	966-970	NNP	denotes	Core
T14183	971-979	NNP	denotes	Facility
T14184	979-980	-COMMA-	denotes	,
T14185	981-984	CC	denotes	and
T14186	985-987	CD	denotes	10
T14187	988-990	NN	denotes	mM
T14188	991-993	IN	denotes	of
T14189	994-999	NN	denotes	stock
T14190	1000-1009	NN	denotes	solutions
T14191	1010-1013	VB	denotes	was
T14192	1014-1022	VB	denotes	prepared
T14193	1023-1025	IN	denotes	in
T14194	1026-1030	NN	denotes	DMSO
T14607	1033-1042	NN	denotes	Isolation
T14608	1043-1046	CC	denotes	and
T14609	1047-1054	NN	denotes	culture
T14610	1055-1057	IN	denotes	of
T14611	1058-1065	JJ	denotes	primary
T14612	1066-1070	JJ	denotes	CD8+
T14613	1071-1072	NN	denotes	T
T14614	1073-1078	NN	denotes	cells
T14615	1080-1084	JJ	denotes	CD8+
T14616	1085-1086	NN	denotes	T
T14617	1087-1092	NN	denotes	cells
T14618	1093-1097	IN	denotes	from
T14619	1098-1108	JJ	denotes	4–8-wk-old
T14620	1109-1116	NN	denotes	Tcra−/−
T14621	1117-1118	NN	denotes	×
T14622	1119-1122	NN	denotes	P14
T14623	1123-1126	NN	denotes	TCR
T14624	1127-1137	JJ	denotes	transgenic
T14625	1138-1139	-LRB-	denotes	(
T14626	1139-1146	JJ	denotes	Taconic
T14627	1146-1147	-RRB-	denotes	)
T14628	1147-1148	-COMMA-	denotes	,
T14629	1149-1157	NN	denotes	C57BL/6J
T14630	1158-1160	NNP	denotes	WT
T14631	1160-1161	-COMMA-	denotes	,
T14632	1162-1164	CC	denotes	or
T14633	1165-1173	NN	denotes	Tbx21−/−
T14634	1174-1175	-LRB-	denotes	(
T14635	1175-1178	DT	denotes	The
T14636	1179-1186	NNP	denotes	Jackson
T14637	1187-1197	NNP	denotes	Laboratory
T14638	1197-1198	-RRB-	denotes	)
T14639	1199-1203	NN	denotes	mice
T14640	1204-1208	VB	denotes	were
T14641	1209-1217	VB	denotes	purified
T14642	1218-1219	-LRB-	denotes	(
T14643	1219-1220	SYM	denotes	>
T14644	1220-1222	CD	denotes	95
T14645	1222-1223	NN	denotes	%
T14646	1224-1230	NN	denotes	purity
T14647	1230-1231	-RRB-	denotes	)
T14648	1232-1234	IN	denotes	by
T14649	1235-1243	JJ	denotes	negative
T14650	1244-1253	NN	denotes	selection
T14651	1254-1255	-LRB-	denotes	(
T14652	1255-1265	NN	denotes	Invitrogen
T14653	1265-1266	-RRB-	denotes	)
T14654	1267-1271	IN	denotes	from
T14655	1272-1278	VB	denotes	pooled
T14656	1279-1285	NN	denotes	spleen
T14657	1286-1289	CC	denotes	and
T14658	1290-1295	NN	denotes	lymph
T14659	1296-1300	NN	denotes	node
T14660	1301-1306	NN	denotes	cells
T14661	1308-1312	JJ	denotes	CD8+
T14662	1313-1314	NN	denotes	T
T14663	1315-1320	NN	denotes	cells
T14664	1321-1325	IN	denotes	from
T14665	1326-1334	NN	denotes	Runx3−/−
T14666	1335-1339	NN	denotes	mice
T14667	1340-1342	IN	denotes	on
T14668	1343-1346	DT	denotes	the
T14669	1347-1350	NN	denotes	ICR
T14670	1351-1361	NN	denotes	background
T14671	1362-1366	VB	denotes	were
T14672	1367-1375	VB	denotes	purified
T14673	1376-1378	IN	denotes	by
T14674	1379-1387	JJ	denotes	positive
T14675	1388-1397	NN	denotes	selection
T14676	1398-1399	-LRB-	denotes	(
T14677	1399-1407	NNP	denotes	Miltenyi
T14678	1408-1414	NNP	denotes	Biotec
T14679	1414-1415	-RRB-	denotes	)
T14680	1417-1420	DT	denotes	All
T14681	1421-1425	NN	denotes	mice
T14682	1426-1430	VB	denotes	were
T14683	1431-1441	VB	denotes	maintained
T14684	1442-1444	IN	denotes	in
T14685	1445-1453	JJ	denotes	specific
T14686	1454-1467	JJ	denotes	pathogen-free
T14687	1468-1475	NN	denotes	barrier
T14688	1476-1486	NN	denotes	facilities
T14689	1487-1490	CC	denotes	and
T14690	1491-1495	VB	denotes	used
T14691	1496-1505	VB	denotes	according
T14692	1506-1508	TO	denotes	to
T14693	1509-1518	NN	denotes	protocols
T14694	1519-1527	VB	denotes	approved
T14695	1528-1530	IN	denotes	by
T14696	1531-1534	DT	denotes	the
T14697	1535-1541	NNP	denotes	Immune
T14698	1542-1549	NNP	denotes	Disease
T14699	1550-1559	NNP	denotes	Institute
T14700	1560-1563	CC	denotes	and
T14701	1564-1567	DT	denotes	the
T14702	1568-1575	NNP	denotes	Harvard
T14703	1576-1583	NNP	denotes	Medical
T14704	1584-1590	NNP	denotes	School
T14705	1591-1597	NNP	denotes	Animal
T14706	1598-1602	NNP	denotes	Care
T14707	1603-1606	CC	denotes	and
T14708	1607-1610	NNP	denotes	Use
T14709	1611-1622	NNP	denotes	Committees.
T14710	1623-1626	IN	denotes	For
T14711	1627-1638	NN	denotes	stimulation
T14712	1638-1639	-COMMA-	denotes	,
T14713	1640-1648	VB	denotes	purified
T14714	1649-1653	JJ	denotes	CD8+
T14715	1654-1655	NN	denotes	T
T14716	1656-1661	NN	denotes	cells
T14717	1662-1666	VB	denotes	were
T14718	1667-1675	VB	denotes	cultured
T14719	1676-1678	IN	denotes	at
T14720	1679-1682	CD	denotes	106
T14721	1683-1691	NN	denotes	cells/ml
T14722	1692-1693	-LRB-	denotes	(
T14723	1693-1695	CD	denotes	10
T14724	1696-1698	NN	denotes	ml
T14725	1698-1699	-RRB-	denotes	)
T14726	1700-1702	IN	denotes	in
T14727	1703-1706	NN	denotes	T25
T14728	1707-1713	NN	denotes	flasks
T14729	1714-1720	VB	denotes	coated
T14730	1721-1725	IN	denotes	with
T14731	1726-1727	CD	denotes	1
T14732	1728-1733	NN	denotes	μg/ml
T14733	1734-1738	DT	denotes	each
T14734	1739-1741	IN	denotes	of
T14735	1742-1750	NN	denotes	anti-CD3
T14736	1751-1752	-LRB-	denotes	(
T14737	1752-1757	NN	denotes	clone
T14738	1758-1762	NN	denotes	2C11
T14739	1762-1763	-RRB-	denotes	)
T14740	1764-1767	CC	denotes	and
T14741	1768-1777	JJ	denotes	anti-CD28
T14742	1778-1779	-LRB-	denotes	(
T14743	1779-1784	NN	denotes	clone
T14744	1785-1790	CD	denotes	37.51
T14745	1790-1791	-RRB-	denotes	)
T14746	1792-1794	IN	denotes	by
T14747	1795-1807	NN	denotes	pretreatment
T14748	1808-1812	IN	denotes	with
T14749	1813-1816	CD	denotes	300
T14750	1817-1822	NN	denotes	μg/ml
T14751	1823-1827	NN	denotes	goat
T14752	1828-1840	NN	denotes	anti–hamster
T14753	1841-1844	NN	denotes	IgG
T14754	1846-1851	IN	denotes	After
T14755	1852-1854	CD	denotes	48
T14756	1855-1856	NN	denotes	h
T14757	1856-1857	-COMMA-	denotes	,
T14758	1858-1863	NN	denotes	cells
T14759	1864-1868	VB	denotes	were
T14760	1869-1876	VB	denotes	removed
T14761	1877-1881	IN	denotes	from
T14762	1882-1885	DT	denotes	the
T14763	1886-1889	NN	denotes	TCR
T14764	1890-1901	NN	denotes	stimulation
T14765	1902-1905	CC	denotes	and
T14766	1906-1916	VB	denotes	recultured
T14767	1917-1919	IN	denotes	at
T14768	1920-1921	DT	denotes	a
T14769	1922-1935	NN	denotes	concentration
T14770	1936-1938	IN	denotes	of
T14771	1939-1940	CD	denotes	5
T14772	1941-1942	IN	denotes	×
T14773	1943-1946	CD	denotes	105
T14774	1947-1955	NN	denotes	cells/ml
T14775	1956-1958	IN	denotes	in
T14776	1959-1964	NN	denotes	media
T14777	1965-1977	VB	denotes	supplemented
T14778	1978-1982	IN	denotes	with
T14779	1983-1986	CD	denotes	100
T14780	1987-1991	NN	denotes	U/ml
T14781	1992-1998	NN	denotes	rhIL-2
T14782	2000-2005	DT	denotes	Every
T14783	2006-2008	CD	denotes	24
T14784	2009-2010	NN	denotes	h
T14785	2010-2011	-COMMA-	denotes	,
T14786	2012-2018	JJ	denotes	viable
T14787	2019-2024	NN	denotes	cells
T14788	2025-2029	VB	denotes	were
T14789	2030-2037	VB	denotes	counted
T14790	2038-2041	CC	denotes	and
T14791	2042-2052	VB	denotes	readjusted
T14792	2053-2055	TO	denotes	to
T14793	2056-2057	CD	denotes	5
T14794	2058-2059	IN	denotes	×
T14795	2060-2063	CD	denotes	105
T14796	2064-2072	NN	denotes	cells/ml
T14797	2073-2077	IN	denotes	with
T14798	2078-2083	JJ	denotes	fresh
T14799	2084-2089	NN	denotes	media
T14800	2090-2100	VB	denotes	containing
T14801	2101-2104	DT	denotes	the
T14802	2105-2118	VB	denotes	corresponding
T14803	2119-2125	NN	denotes	amount
T14804	2126-2128	IN	denotes	of
T14805	2129-2135	NN	denotes	rhIL-2
T15411	2138-2147	NN	denotes	Isolation
T15412	2148-2150	IN	denotes	of
T15413	2151-2155	JJ	denotes	CD8+
T15414	2156-2157	NN	denotes	T
T15415	2158-2163	NN	denotes	cells
T15416	2164-2168	IN	denotes	from
T15417	2169-2177	NN	denotes	Runx3−/−
T15418	2178-2182	NN	denotes	mice
T15419	2184-2199	JJ	denotes	Runx3-deficient
T15420	2200-2201	NN	denotes	T
T15421	2202-2207	NN	denotes	cells
T15422	2208-2212	VB	denotes	fail
T15423	2213-2215	TO	denotes	to
T15424	2216-2223	NN	denotes	silence
T15425	2224-2227	NN	denotes	CD4
T15426	2228-2238	NN	denotes	expression
T15427	2239-2247	RB	denotes	normally
T15428	2248-2249	-LRB-	denotes	(
T15429	2249-2253	NNP	denotes	Fig.
T15430	2254-2256	NN	denotes	S1
T15431	2256-2257	-RRB-	denotes	)
T15432	2258-2259	-LRB-	denotes	(
T15433	2259-2261	CD	denotes	12
T15434	2261-2262	-COMMA-	denotes	,
T15435	2263-2265	CD	denotes	13
T15436	2265-2266	-RRB-	denotes	)
T15437	2268-2270	PRP	denotes	We
T15438	2271-2280	RB	denotes	therefore
T15439	2281-2288	RB	denotes	further
T15440	2289-2301	VB	denotes	fractionated
T15441	2302-2305	DT	denotes	the
T15442	2306-2316	RB	denotes	positively
T15443	2317-2325	VB	denotes	selected
T15444	2326-2330	JJ	denotes	CD8+
T15445	2331-2332	NN	denotes	T
T15446	2333-2338	NN	denotes	cells
T15447	2339-2343	IN	denotes	from
T15448	2344-2349	NN	denotes	Runx3
T15449	2350-2352	NN	denotes	KO
T15450	2353-2357	NN	denotes	mice
T15451	2358-2362	IN	denotes	into
T15452	2363-2371	NN	denotes	CD8+CD4−
T15453	2372-2374	NN	denotes	SP
T15454	2375-2377	CC	denotes	or
T15455	2378-2386	JJ	denotes	CD8+CD4+
T15456	2387-2389	NN	denotes	DP
T15457	2390-2395	NN	denotes	cells
T15458	2396-2398	IN	denotes	by
T15459	2399-2409	NN	denotes	separation
T15460	2410-2415	VB	denotes	using
T15461	2416-2424	JJ	denotes	anti-CD4
T15462	2425-2433	JJ	denotes	magnetic
T15463	2434-2439	NN	denotes	beads
T15464	2441-2445	DT	denotes	This
T15465	2446-2453	VB	denotes	yielded
T15466	2454-2455	DT	denotes	a
T15467	2456-2461	NN	denotes	Runx3
T15468	2462-2464	NN	denotes	KO
T15469	2465-2467	NN	denotes	SP
T15470	2468-2478	VB	denotes	“enriched”
T15471	2479-2489	NN	denotes	population
T15472	2490-2494	WDT	denotes	that
T15473	2495-2504	VB	denotes	contained
T15474	2505-2507	CD	denotes	75
T15475	2507-2508	NN	denotes	%
T15476	2509-2517	NN	denotes	CD8+CD4−
T15477	2518-2523	NN	denotes	cells
T15478	2524-2527	CC	denotes	and
T15479	2528-2529	DT	denotes	a
T15480	2530-2532	NN	denotes	KO
T15481	2533-2535	NN	denotes	DP
T15482	2536-2544	VB	denotes	enriched
T15483	2545-2555	NN	denotes	population
T15484	2556-2560	WDT	denotes	that
T15485	2561-2570	VB	denotes	contained
T15486	2571-2573	CD	denotes	85
T15487	2573-2574	NN	denotes	%
T15488	2575-2583	JJ	denotes	CD8+CD4+
T15489	2584-2589	NN	denotes	cells
T15490	2590-2591	-LRB-	denotes	(
T15491	2591-2595	NN	denotes	Fig.
T15492	2596-2598	NN	denotes	S1
T15493	2598-2599	-RRB-	denotes	)
T15494	2601-2604	DT	denotes	The
T15495	2605-2610	NN	denotes	cells
T15496	2611-2615	VB	denotes	were
T15497	2616-2626	VB	denotes	stimulated
T15498	2627-2631	IN	denotes	with
T15499	2632-2641	JJ	denotes	anti-CD3+
T15500	2642-2651	NN	denotes	anti-CD28
T15501	2652-2655	IN	denotes	for
T15502	2656-2657	CD	denotes	2
T15503	2658-2659	NN	denotes	d
T15504	2660-2666	IN	denotes	before
T15505	2667-2675	VB	denotes	removing
T15506	2676-2680	PRP	denotes	them
T15507	2681-2685	IN	denotes	from
T15508	2686-2689	DT	denotes	the
T15509	2690-2693	NN	denotes	TCR
T15510	2694-2702	NN	denotes	stimulus
T15511	2703-2706	CC	denotes	and
T15512	2707-2716	VB	denotes	culturing
T15513	2717-2721	PRP	denotes	them
T15514	2722-2724	IN	denotes	in
T15515	2725-2730	NN	denotes	media
T15516	2731-2741	VB	denotes	containing
T15517	2742-2745	CD	denotes	100
T15518	2746-2750	NN	denotes	U/ml
T15519	2751-2755	NN	denotes	IL-2
T15520	2757-2759	IN	denotes	As
T15521	2760-2770	RB	denotes	previously
T15522	2771-2779	VB	denotes	reported
T15523	2779-2780	-COMMA-	denotes	,
T15524	2781-2792	JJ	denotes	TCR-induced
T15525	2793-2806	NN	denotes	proliferation
T15526	2807-2809	IN	denotes	of
T15527	2810-2818	NN	denotes	Runx3−/−
T15528	2819-2823	JJ	denotes	CD8+
T15529	2824-2825	NN	denotes	T
T15530	2826-2831	NN	denotes	cells
T15531	2832-2835	VB	denotes	was
T15532	2836-2844	RB	denotes	severely
T15533	2845-2853	JJ	denotes	impaired
T15534	2853-2854	-COMMA-	denotes	,
T15535	2855-2867	JJ	denotes	irrespective
T15536	2868-2870	IN	denotes	of
T15537	2871-2874	NN	denotes	CD4
T15538	2875-2885	NN	denotes	expression
T15539	2886-2887	-LRB-	denotes	(
T15540	2887-2891	NN	denotes	Fig.
T15541	2892-2894	NN	denotes	S1
T15542	2894-2895	-RRB-	denotes	)
T15543	2896-2897	-LRB-	denotes	(
T15544	2897-2899	CD	denotes	12
T15545	2899-2900	-COMMA-	denotes	,
T15546	2901-2903	CD	denotes	13
T15547	2903-2904	-RRB-	denotes	)
T15548	2906-2913	RB	denotes	However
T15549	2913-2914	-COMMA-	denotes	,
T15550	2915-2918	DT	denotes	the
T15551	2919-2927	NN	denotes	Runx3−/−
T15552	2928-2933	NN	denotes	cells
T15553	2934-2940	VB	denotes	showed
T15554	2941-2953	JJ	denotes	cell-surface
T15555	2954-2964	NN	denotes	expression
T15556	2965-2973	NN	denotes	patterns
T15557	2974-2984	JJ	denotes	indicative
T15558	2985-2987	IN	denotes	of
T15559	2988-2997	VB	denotes	activated
T15560	2998-3003	NN	denotes	cells
T15561	3003-3004	-COMMA-	denotes	,
T15562	3005-3014	VB	denotes	including
T15563	3015-3028	NN	denotes	up-regulation
T15564	3029-3031	IN	denotes	of
T15565	3032-3036	NN	denotes	CD25
T15566	3037-3040	CC	denotes	and
T15567	3041-3045	NN	denotes	CD69
T15568	3046-3047	-LRB-	denotes	(
T15569	3047-3051	NN	denotes	Fig.
T15570	3052-3054	NN	denotes	S1
T15571	3054-3055	-RRB-	denotes	)
T15572	3057-3059	IN	denotes	As
T15573	3060-3068	VB	denotes	expected
T15574	3069-3073	IN	denotes	from
T15575	3074-3079	PRP-DOLLAR-	denotes	their
T15576	3080-3087	NN	denotes	ability
T15577	3088-3090	TO	denotes	to
T15578	3091-3102	VB	denotes	up-regulate
T15579	3103-3107	NN	denotes	CD25
T15580	3107-3108	-COMMA-	denotes	,
T15581	3109-3117	NN	denotes	Runx3−/−
T15582	3118-3122	JJ	denotes	CD8+
T15583	3123-3124	NN	denotes	T
T15584	3125-3130	NN	denotes	cells
T15585	3131-3140	VB	denotes	responded
T15586	3141-3143	TO	denotes	to
T15587	3144-3148	NN	denotes	IL-2
T15588	3149-3164	NN	denotes	supplementation
T15589	3165-3170	IN	denotes	after
T15590	3171-3174	NN	denotes	day
T15591	3175-3176	CD	denotes	2
T15592	3177-3180	CC	denotes	and
T15593	3181-3192	RB	denotes	efficiently
T15594	3193-3201	VB	denotes	expanded
T15595	3202-3207	IN	denotes	until
T15596	3208-3211	NN	denotes	day
T15597	3212-3213	CD	denotes	6
T15598	3214-3216	IN	denotes	of
T15599	3217-3220	DT	denotes	the
T15600	3221-3228	NN	denotes	culture
T15601	3229-3235	NN	denotes	period
T15602	3235-3236	-COMMA-	denotes	,
T15603	3237-3243	IN	denotes	albeit
T15604	3244-3246	IN	denotes	at
T15605	3247-3253	JJ	denotes	slower
T15606	3254-3259	NN	denotes	rates
T15607	3260-3268	VB	denotes	compared
T15608	3269-3273	IN	denotes	with
T15609	3274-3276	JJ	denotes	WT
T15610	3277-3282	NN	denotes	cells
T15611	3283-3284	-LRB-	denotes	(
T15612	3284-3288	NN	denotes	Fig.
T15613	3289-3291	NN	denotes	S1
T15614	3291-3292	-RRB-	denotes	)
T15615	3294-3302	IN	denotes	Although
T15616	3303-3304	DT	denotes	a
T15617	3305-3313	NN	denotes	fraction
T15618	3314-3316	IN	denotes	of
T15619	3317-3320	DT	denotes	the
T15620	3321-3323	NN	denotes	KO
T15621	3324-3326	NN	denotes	DP
T15622	3327-3332	NN	denotes	cells
T15623	3333-3341	VB	denotes	silenced
T15624	3342-3345	NN	denotes	CD4
T15625	3346-3356	NN	denotes	expression
T15626	3357-3362	IN	denotes	after
T15627	3363-3373	NN	denotes	activation
T15628	3373-3374	-COMMA-	denotes	,
T15629	3375-3378	DT	denotes	the
T15630	3379-3384	NN	denotes	ratio
T15631	3385-3387	IN	denotes	of
T15632	3388-3393	NN	denotes	SP/DP
T15633	3394-3399	NN	denotes	cells
T15634	3400-3402	IN	denotes	in
T15635	3403-3407	DT	denotes	each
T15636	3408-3416	VB	denotes	enriched
T15637	3417-3427	NN	denotes	population
T15638	3428-3436	VB	denotes	remained
T15639	3437-3445	JJ	denotes	constant
T15640	3446-3456	RB	denotes	thereafter
T15641	3456-3457	-COMMA-	denotes	,
T15642	3458-3461	CC	denotes	and
T15643	3462-3464	PRP	denotes	we
T15644	3465-3468	VB	denotes	did
T15645	3469-3472	RB	denotes	not
T15646	3473-3480	VB	denotes	observe
T15647	3481-3484	DT	denotes	any
T15648	3485-3490	JJ	denotes	major
T15649	3491-3502	NN	denotes	differences
T15650	3503-3510	IN	denotes	between
T15651	3511-3516	DT	denotes	these
T15652	3517-3520	CD	denotes	two
T15653	3521-3532	NN	denotes	populations
T15654	3533-3543	IN	denotes	throughout
T15655	3544-3547	DT	denotes	the
T15656	3548-3555	NN	denotes	culture
T15657	3556-3562	NN	denotes	period
T15658	3562-3563	-COMMA-	denotes	,
T15659	3564-3574	VB	denotes	indicating
T15660	3575-3579	IN	denotes	that
T15661	3580-3582	IN	denotes	in
T15662	3583-3588	NN	denotes	terms
T15663	3589-3591	IN	denotes	of
T15664	3592-3600	NN	denotes	effector
T15665	3601-3604	NN	denotes	CTL
T15666	3605-3620	NN	denotes	differentiation
T15667	3621-3624	CC	denotes	and
T15668	3625-3630	IN	denotes	under
T15669	3631-3634	PRP-DOLLAR-	denotes	our
T15670	3635-3642	NN	denotes	culture
T15671	3643-3653	NN	denotes	conditions
T15672	3653-3654	-COMMA-	denotes	,
T15673	3655-3663	NN	denotes	Runx3−/−
T15674	3664-3668	JJ	denotes	CD8+
T15675	3669-3670	NN	denotes	T
T15676	3671-3676	NN	denotes	cells
T15677	3677-3681	WDT	denotes	that
T15678	3682-3686	RB	denotes	also
T15679	3687-3696	VB	denotes	coexpress
T15680	3697-3700	NN	denotes	CD4
T15681	3701-3704	VB	denotes	are
T15682	3705-3722	JJ	denotes	indistinguishable
T15683	3723-3727	IN	denotes	from
T15684	3728-3733	DT	denotes	those
T15685	3734-3738	WDT	denotes	that
T15686	3739-3741	VB	denotes	do
T15687	3742-3745	RB	denotes	not
T15688	3747-3750	DT	denotes	The
T15689	3751-3755	NN	denotes	data
T15690	3756-3765	VB	denotes	presented
T15691	3766-3768	IN	denotes	in
T15692	3769-3773	NNP	denotes	Fig.
T15693	3774-3776	NN	denotes	S2
T15694	3777-3780	VB	denotes	are
T15695	3781-3785	IN	denotes	from
T15696	3786-3791	NN	denotes	Runx3
T15697	3792-3794	NN	denotes	KO
T15698	3795-3797	NN	denotes	SP
T15699	3798-3803	NN	denotes	cells
T15700	3803-3804	-COMMA-	denotes	,
T15701	3805-3812	IN	denotes	whereas
T15702	3813-3818	DT	denotes	those
T15703	3819-3824	VB	denotes	shown
T15704	3825-3827	IN	denotes	in
T15705	3828-3833	NNP	denotes	Figs.
T15706	3834-3835	CD	denotes	3
T15707	3836-3839	CC	denotes	and
T15708	3840-3841	CD	denotes	4
T15709	3842-3845	VB	denotes	are
T15710	3846-3850	IN	denotes	from
T15711	3851-3856	JJ	denotes	total
T15712	3857-3862	NN	denotes	Runx3
T15713	3863-3865	NN	denotes	KO
T15714	3866-3869	NN	denotes	CD8
T15715	3870-3875	NN	denotes	cells
T16229	3878-3888	JJ	denotes	FACS-based
T16230	3889-3901	NN	denotes	cytotoxicity
T16231	3902-3907	NN	denotes	assay
T16232	3909-3911	TO	denotes	To
T16233	3912-3919	VB	denotes	measure
T16234	3920-3932	NN	denotes	cytotoxicity
T16235	3932-3933	-COMMA-	denotes	,
T16236	3934-3937	NN	denotes	EL4
T16237	3938-3945	NN	denotes	thymoma
T16238	3946-3952	NN	denotes	target
T16239	3953-3958	NN	denotes	cells
T16240	3959-3963	VB	denotes	were
T16241	3964-3970	VB	denotes	loaded
T16242	3971-3975	IN	denotes	with
T16243	3976-3977	CD	denotes	0
T16244	3978-3980	CC	denotes	or
T16245	3981-3982	CD	denotes	1
T16246	3983-3985	NN	denotes	μM
T16247	3986-3990	NN	denotes	Gp33
T16248	3991-3998	NN	denotes	peptide
T16249	3999-4002	IN	denotes	for
T16250	4003-4004	CD	denotes	2
T16251	4005-4006	NN	denotes	h
T16252	4007-4013	IN	denotes	before
T16253	4014-4015	DT	denotes	a
T16254	4016-4019	JJ	denotes	2-h
T16255	4020-4032	NN	denotes	coincubation
T16256	4033-4037	IN	denotes	with
T16257	4038-4041	NN	denotes	P14
T16258	4042-4046	JJ	denotes	CD8+
T16259	4047-4048	NN	denotes	T
T16260	4049-4054	NN	denotes	cells
T16261	4055-4057	IN	denotes	at
T16262	4058-4061	DT	denotes	the
T16263	4062-4080	JJ	denotes	effector-to-target
T16264	4081-4087	NN	denotes	ratios
T16265	4088-4097	VB	denotes	indicated
T16266	4098-4100	IN	denotes	in
T16267	4101-4104	DT	denotes	the
T16268	4105-4112	NN	denotes	figures
T16269	4113-4115	IN	denotes	in
T16270	4116-4123	JJ	denotes	96-well
T16271	4124-4136	JJ	denotes	round-bottom
T16272	4137-4143	NN	denotes	plates
T16273	4145-4150	IN	denotes	After
T16274	4151-4154	DT	denotes	the
T16275	4155-4167	NN	denotes	coincubation
T16276	4168-4174	NN	denotes	period
T16277	4174-4175	-COMMA-	denotes	,
T16278	4176-4181	NN	denotes	cells
T16279	4182-4186	VB	denotes	were
T16280	4187-4194	VB	denotes	stained
T16281	4195-4199	IN	denotes	with
T16282	4200-4207	NN	denotes	Annexin
T16283	4208-4214	NN	denotes	V–FITC
T16284	4215-4218	CC	denotes	and
T16285	4219-4243	NN	denotes	anti-CD8–allophycocyanin
T16286	4245-4249	NN	denotes	Data
T16287	4250-4258	NN	denotes	analysis
T16288	4259-4262	VB	denotes	was
T16289	4263-4272	VB	denotes	performed
T16290	4273-4277	IN	denotes	with
T16291	4278-4284	NN	denotes	FlowJo
T16292	4285-4293	NN	denotes	software
T16293	4294-4295	-LRB-	denotes	(
T16294	4295-4299	NNP	denotes	Tree
T16295	4300-4304	NNP	denotes	Star
T16296	4304-4305	-COMMA-	denotes	,
T16297	4306-4310	NNP	denotes	Inc.
T16298	4310-4311	-RRB-	denotes	)
T16299	4311-4312	-COLON-	denotes	;
T16300	4313-4316	NN	denotes	EL4
T16301	4317-4323	NN	denotes	target
T16302	4324-4329	NN	denotes	cells
T16303	4330-4331	-LRB-	denotes	(
T16304	4331-4343	JJ	denotes	CD8-negative
T16305	4344-4350	NN	denotes	events
T16306	4350-4351	-RRB-	denotes	)
T16307	4352-4356	VB	denotes	were
T16308	4357-4362	VB	denotes	gated
T16309	4362-4363	-COMMA-	denotes	,
T16310	4364-4367	CC	denotes	and
T16311	4368-4371	DT	denotes	the
T16312	4372-4382	NN	denotes	percentage
T16313	4383-4385	IN	denotes	of
T16314	4386-4393	NN	denotes	Annexin
T16315	4394-4396	JJ	denotes	V+
T16316	4397-4403	NN	denotes	target
T16317	4404-4409	NN	denotes	cells
T16318	4410-4413	VB	denotes	was
T16319	4414-4424	VB	denotes	determined
T16510	4427-4435	NN	denotes	Cytokine
T16511	4436-4439	CC	denotes	and
T16512	4440-4447	NN	denotes	surface
T16513	4448-4454	NN	denotes	marker
T16514	4455-4463	NN	denotes	staining
T16515	4465-4467	TO	denotes	To
T16516	4468-4474	VB	denotes	assess
T16517	4475-4483	NN	denotes	cytokine
T16518	4484-4494	NN	denotes	production
T16519	4494-4495	-COMMA-	denotes	,
T16520	4496-4501	NN	denotes	cells
T16521	4502-4506	VB	denotes	were
T16522	4507-4519	VB	denotes	restimulated
T16523	4520-4524	IN	denotes	with
T16524	4525-4527	CD	denotes	10
T16525	4528-4530	NN	denotes	nM
T16526	4531-4534	NN	denotes	PMA
T16527	4535-4536	SYM	denotes	+
T16528	4537-4538	CD	denotes	1
T16529	4539-4541	NN	denotes	μM
T16530	4542-4551	NN	denotes	ionomycin
T16531	4552-4555	IN	denotes	for
T16532	4556-4557	CD	denotes	6
T16533	4558-4559	NN	denotes	h
T16534	4560-4561	-LRB-	denotes	(
T16535	4561-4567	IN	denotes	unless
T16536	4568-4577	VB	denotes	indicated
T16537	4578-4587	RB	denotes	otherwise
T16538	4588-4590	IN	denotes	in
T16539	4591-4594	DT	denotes	the
T16540	4595-4602	NN	denotes	figures
T16541	4602-4603	-RRB-	denotes	)
T16542	4603-4604	-COMMA-	denotes	,
T16543	4605-4608	CC	denotes	and
T16544	4609-4622	JJ	denotes	intracellular
T16545	4623-4631	NN	denotes	cytokine
T16546	4632-4638	NN	denotes	stains
T16547	4639-4643	VB	denotes	were
T16548	4644-4653	VB	denotes	performed
T16549	4654-4656	IN	denotes	as
T16550	4657-4667	RB	denotes	previously
T16551	4668-4677	VB	denotes	described
T16552	4678-4679	-LRB-	denotes	(
T16553	4679-4681	CD	denotes	28
T16554	4681-4682	-RRB-	denotes	)
T16555	4684-4686	TO	denotes	To
T16556	4687-4693	VB	denotes	detect
T16557	4694-4704	NN	denotes	expression
T16558	4705-4707	IN	denotes	of
T16559	4708-4715	NN	denotes	surface
T16560	4716-4725	NN	denotes	molecules
T16561	4725-4726	-COMMA-	denotes	,
T16562	4727-4732	NN	denotes	cells
T16563	4733-4737	VB	denotes	were
T16564	4738-4744	VB	denotes	washed
T16565	4745-4747	IN	denotes	in
T16566	4748-4751	NNP	denotes	PBS
T16567	4751-4752	-COMMA-	denotes	,
T16568	4753-4764	VB	denotes	resuspended
T16569	4765-4767	IN	denotes	in
T16570	4768-4772	NN	denotes	FACS
T16571	4773-4777	NN	denotes	wash
T16572	4778-4784	NN	denotes	buffer
T16573	4785-4786	-LRB-	denotes	(
T16574	4786-4787	CD	denotes	3
T16575	4787-4788	NN	denotes	%
T16576	4789-4792	NN	denotes	FBS
T16577	4792-4793	-COMMA-	denotes	,
T16578	4794-4797	CD	denotes	0.1
T16579	4797-4798	NN	denotes	%
T16580	4799-4805	NN	denotes	sodium
T16581	4806-4811	NN	denotes	azide
T16582	4811-4812	-COMMA-	denotes	,
T16583	4813-4815	CD	denotes	30
T16584	4816-4818	NN	denotes	mM
T16585	4819-4824	NNP	denotes	Hepes
T16586	4824-4825	-COMMA-	denotes	,
T16587	4826-4828	CD	denotes	1×
T16588	4829-4832	NNP	denotes	PBS
T16589	4832-4833	-RRB-	denotes	)
T16590	4834-4844	VB	denotes	containing
T16591	4845-4848	DT	denotes	the
T16592	4849-4859	NN	denotes	antibodies
T16593	4860-4869	VB	denotes	indicated
T16594	4870-4872	IN	denotes	in
T16595	4873-4876	DT	denotes	the
T16596	4877-4884	NN	denotes	figures
T16597	4885-4887	IN	denotes	at
T16609	4966-4967	-LRB-	denotes	(
T16610	4967-4969	NN	denotes	RT
T16611	4969-4970	-RRB-	denotes	)
T16612	4970-4971	-COMMA-	denotes	,
T16613	4972-4978	VB	denotes	washed
T16614	4978-4979	-COMMA-	denotes	,
T16615	4980-4983	CC	denotes	and
T16616	4984-4995	VB	denotes	resuspended
T16617	4996-4998	IN	denotes	in
T16618	4999-5000	CD	denotes	2
T16619	5000-5001	NN	denotes	%
T16620	5002-5014	NN	denotes	formaldehyde
T16621	5015-5023	JJ	denotes	fixative
T16622	5024-5032	NN	denotes	solution
T16623	5033-5039	IN	denotes	before
T16624	5040-5051	NN	denotes	acquisition
T16625	5052-5054	IN	denotes	on
T16626	5055-5056	DT	denotes	a
T16627	5057-5068	NN	denotes	FACSCalibur
T16628	5069-5070	-LRB-	denotes	(
T16629	5070-5072	NN	denotes	BD
T16630	5072-5073	-RRB-	denotes	)
T16785	5076-5086	JJ	denotes	Retroviral
T16786	5087-5099	NN	denotes	transduction
T16787	5100-5102	IN	denotes	of
T16788	5103-5110	JJ	denotes	primary
T16789	5111-5115	JJ	denotes	CD8+
T16790	5116-5117	NN	denotes	T
T16791	5118-5123	NN	denotes	cells
T16792	5125-5128	IN	denotes	For
T16793	5129-5141	NN	denotes	transduction
T16794	5142-5153	NN	denotes	experiments
T16795	5153-5154	-COMMA-	denotes	,
T16796	5155-5160	JJ	denotes	viral
T16797	5161-5173	NN	denotes	supernatants
T16798	5174-5178	VB	denotes	were
T16799	5179-5188	VB	denotes	generated
T16800	5189-5191	IN	denotes	by
T16801	5192-5199	NN	denotes	calcium
T16802	5200-5209	NN	denotes	phosphate
T16803	5210-5222	NN	denotes	transfection
T16804	5223-5225	IN	denotes	of
T16805	5226-5233	NN	denotes	Phoenix
T16806	5234-5239	NN	denotes	cells
T16807	5240-5243	CC	denotes	and
T16808	5244-5257	NN	denotes	concentration
T16809	5258-5260	IN	denotes	by
T16810	5261-5270	JJ	denotes	overnight
T16811	5271-5285	NN	denotes	centrifugation
T16812	5286-5288	IN	denotes	at
T16813	5289-5294	CD	denotes	6,000
T16814	5295-5296	NN	denotes	g
T16815	5298-5300	IN	denotes	At
T16816	5301-5304	CD	denotes	∼42
T16817	5305-5306	NN	denotes	h
T16818	5307-5312	IN	denotes	after
T16819	5313-5316	DT	denotes	the
T16820	5317-5324	JJ	denotes	initial
T16821	5325-5328	NN	denotes	TCR
T16822	5329-5339	NN	denotes	activation
T16823	5340-5342	IN	denotes	of
T16824	5343-5346	CD	denotes	106
T16825	5347-5351	JJ	denotes	CD8+
T16826	5352-5353	NN	denotes	T
T16827	5354-5359	NN	denotes	cells
T16828	5360-5363	IN	denotes	per
T16829	5364-5368	RB	denotes	well
T16830	5369-5371	IN	denotes	in
T16831	5372-5379	JJ	denotes	12-well
T16832	5380-5386	NN	denotes	plates
T16833	5386-5387	-COMMA-	denotes	,
T16834	5388-5391	DT	denotes	the
T16835	5392-5399	NN	denotes	culture
T16836	5400-5405	NN	denotes	media
T16837	5406-5409	VB	denotes	was
T16838	5410-5417	VB	denotes	removed
T16839	5418-5421	CC	denotes	and
T16840	5422-5430	VB	denotes	replaced
T16841	5431-5435	IN	denotes	with
T16842	5436-5444	JJ	denotes	complete
T16843	5445-5450	NN	denotes	media
T16844	5451-5463	VB	denotes	supplemented
T16845	5464-5468	IN	denotes	with
T16846	5469-5470	CD	denotes	8
T16847	5471-5476	NN	denotes	μg/ml
T16848	5477-5486	NN	denotes	polybrene
T16849	5487-5497	VB	denotes	containing
T16850	5498-5503	JJ	denotes	fresh
T16851	5504-5508	CC	denotes	plus
T16852	5509-5521	JJ	denotes	concentrated
T16853	5522-5527	NN	denotes	virus
T16854	5529-5532	DT	denotes	The
T16855	5533-5539	NN	denotes	plates
T16856	5540-5544	VB	denotes	were
T16857	5545-5556	VB	denotes	centrifuged
T16858	5557-5559	IN	denotes	at
T16859	5560-5563	CD	denotes	700
T16860	5564-5565	NN	denotes	g
T16861	5566-5569	IN	denotes	for
T16862	5570-5571	CD	denotes	1
T16863	5572-5573	NN	denotes	h
T16864	5574-5576	IN	denotes	at
T16865	5577-5579	NN	denotes	RT
T16866	5580-5586	IN	denotes	before
T16867	5587-5596	VB	denotes	returning
T16868	5597-5599	TO	denotes	to
T16869	5600-5604	NN	denotes	37°C
T16870	5605-5608	IN	denotes	for
T16871	5609-5611	DT	denotes	an
T16872	5612-5622	JJ	denotes	additional
T16873	5623-5624	CD	denotes	5
T16874	5625-5626	NN	denotes	h
T16875	5628-5638	JJ	denotes	Retroviral
T16876	5639-5649	NN	denotes	constructs
T16877	5650-5653	IN	denotes	for
T16878	5654-5664	NN	denotes	Eomes-VP16
T16879	5665-5668	CC	denotes	and
T16880	5669-5672	DT	denotes	the
T16881	5673-5676	NN	denotes	MIG
T16882	5677-5684	NN	denotes	control
T16883	5685-5690	JJ	denotes	empty
T16884	5691-5697	NN	denotes	vector
T16885	5698-5702	VB	denotes	were
T16886	5703-5704	DT	denotes	a
T16887	5705-5709	NN	denotes	gift
T16888	5710-5714	IN	denotes	from
T16889	5715-5719	NNP	denotes	S.L.
T16890	5720-5726	NNP	denotes	Reiner
T16891	5727-5728	-LRB-	denotes	(
T16892	5728-5738	NNP	denotes	University
T16893	5739-5741	IN	denotes	of
T16894	5742-5754	NNP	denotes	Pennsylvania
T16895	5754-5755	-COMMA-	denotes	,
T16896	5756-5768	NNP	denotes	Philadelphia
T16897	5768-5769	-COMMA-	denotes	,
T16898	5770-5772	NNP	denotes	PA
T16899	5772-5773	-RRB-	denotes	)
T16900	5774-5775	-LRB-	denotes	(
T16901	5775-5776	CD	denotes	8
T16902	5776-5777	-RRB-	denotes	)
T16963	5780-5784	NN	denotes	ChIP
T16964	5785-5788	CC	denotes	and
T16965	5789-5798	JJ	denotes	real-time
T16966	5799-5802	NN	denotes	PCR
T16967	5803-5811	NN	denotes	analysis
T16968	5813-5815	CD	denotes	20
T16969	5816-5817	CC	denotes	×
T16970	5818-5821	CD	denotes	106
T16971	5822-5826	JJ	denotes	CD8+
T16972	5827-5828	NN	denotes	T
T16973	5829-5834	NN	denotes	cells
T16974	5835-5838	IN	denotes	per
T16975	5839-5858	NN	denotes	immunoprecipitation
T16976	5859-5863	VB	denotes	were
T16977	5864-5869	VB	denotes	fixed
T16978	5870-5872	IN	denotes	by
T16979	5873-5879	VB	denotes	adding
T16980	5880-5881	DT	denotes	a
T16981	5882-5888	JJ	denotes	1/10th
T16982	5889-5895	NN	denotes	volume
T16983	5896-5898	IN	denotes	of
T16984	5899-5907	NN	denotes	fixation
T16985	5908-5916	NN	denotes	solution
T16986	5917-5918	-LRB-	denotes	(
T16987	5918-5922	CD	denotes	11.1
T16988	5922-5923	NN	denotes	%
T16989	5924-5936	NN	denotes	formaldehyde
T16990	5936-5937	-COMMA-	denotes	,
T16991	5938-5941	CD	denotes	100
T16992	5942-5944	NN	denotes	mM
T16993	5945-5949	NN	denotes	NaCl
T16994	5949-5950	-COMMA-	denotes	,
T16995	5951-5952	CD	denotes	1
T16996	5953-5955	NN	denotes	mM
T16997	5956-5960	NN	denotes	EDTA
T16998	5960-5961	-COMMA-	denotes	,
T16999	5962-5965	CD	denotes	0.5
T17000	5966-5968	NN	denotes	mM
T17001	5969-5973	NN	denotes	EGTA
T17002	5973-5974	-COMMA-	denotes	,
T17003	5975-5977	CD	denotes	50
T17004	5978-5980	NN	denotes	mM
T17005	5981-5986	NN	denotes	Hepes
T17006	5986-5987	-RRB-	denotes	)
T17007	5988-5990	TO	denotes	to
T17008	5991-5992	CD	denotes	1
T17009	5993-5999	NN	denotes	volume
T17010	6000-6002	IN	denotes	of
T17011	6003-6010	NN	denotes	culture
T17012	6011-6016	NN	denotes	media
T17013	6017-6020	CC	denotes	and
T17014	6021-6025	VB	denotes	were
T17015	6026-6035	VB	denotes	incubated
T17016	6036-6039	IN	denotes	for
T17017	6040-6042	CD	denotes	10
T17018	6043-6045	CC	denotes	or
T17019	6046-6048	CD	denotes	30
T17020	6049-6052	NN	denotes	min
T17021	6053-6055	IN	denotes	at
T17022	6056-6058	NN	denotes	RT
T17023	6060-6068	NN	denotes	Fixation
T17024	6069-6072	VB	denotes	was
T17025	6073-6080	VB	denotes	stopped
T17026	6081-6085	IN	denotes	with
T17027	6086-6089	CD	denotes	120
T17028	6090-6092	NN	denotes	mM
T17029	6093-6100	NN	denotes	glycine
T17030	6101-6103	IN	denotes	on
T17031	6104-6107	NN	denotes	ice
T17032	6108-6111	IN	denotes	for
T17033	6112-6113	CD	denotes	5
T17034	6114-6117	NN	denotes	min
T17035	6119-6124	VB	denotes	Fixed
T17036	6125-6130	NN	denotes	cells
T17037	6131-6135	VB	denotes	were
T17038	6136-6142	VB	denotes	washed
T17039	6143-6145	CD	denotes	2×
T17040	6146-6150	IN	denotes	with
T17041	6151-6155	JJ	denotes	cold
T17042	6156-6159	NNP	denotes	PBS
T17043	6159-6160	-COMMA-	denotes	,
T17044	6161-6163	CD	denotes	1×
T17045	6164-6168	IN	denotes	with
T17046	6169-6173	JJ	denotes	cold
T17047	6174-6182	NN	denotes	solution
T17048	6183-6184	CD	denotes	I
T17049	6185-6186	-LRB-	denotes	(
T17050	6186-6188	CD	denotes	10
T17051	6189-6191	NN	denotes	mM
T17052	6192-6196	NN	denotes	Tris
T17053	6197-6198	-LRB-	denotes	[
T17054	6198-6200	NN	denotes	pH
T17055	6201-6204	CD	denotes	7.5
T17056	6204-6205	-RRB-	denotes	]
T17057	6205-6206	-COMMA-	denotes	,
T17058	6207-6209	CD	denotes	10
T17059	6210-6212	NN	denotes	mM
T17060	6213-6217	NN	denotes	EDTA
T17061	6217-6218	-COMMA-	denotes	,
T17062	6219-6222	CD	denotes	0.5
T17063	6223-6225	NN	denotes	mM
T17064	6226-6230	NN	denotes	EGTA
T17065	6230-6231	-COMMA-	denotes	,
T17066	6232-6233	CD	denotes	1
T17067	6233-6234	NN	denotes	%
T17068	6235-6241	NN	denotes	Triton
T17069	6242-6247	NN	denotes	X-100
T17070	6247-6248	-RRB-	denotes	)
T17071	6248-6249	-COMMA-	denotes	,
T17072	6250-6253	CC	denotes	and
T17073	6254-6256	CD	denotes	1×
T17074	6257-6261	IN	denotes	with
T17075	6262-6266	JJ	denotes	cold
T17076	6267-6275	NN	denotes	solution
T17077	6276-6278	CD	denotes	II
T17078	6279-6280	-LRB-	denotes	(
T17079	6280-6282	CD	denotes	10
T17080	6283-6285	NN	denotes	mM
T17081	6286-6290	NN	denotes	Tris
T17082	6291-6292	-LRB-	denotes	[
T17083	6292-6294	NN	denotes	pH
T17084	6295-6298	CD	denotes	7.5
T17085	6298-6299	-RRB-	denotes	]
T17086	6299-6300	-COMMA-	denotes	,
T17087	6301-6302	CD	denotes	1
T17088	6303-6305	NN	denotes	mM
T17089	6306-6310	NN	denotes	EDTA
T17090	6310-6311	-COMMA-	denotes	,
T17091	6312-6315	CD	denotes	0.5
T17092	6316-6318	NN	denotes	mM
T17093	6319-6323	NN	denotes	EGTA
T17094	6323-6324	-COMMA-	denotes	,
T17095	6325-6328	CD	denotes	200
T17096	6329-6331	NN	denotes	mM
T17097	6332-6336	NN	denotes	NaCl
T17098	6336-6337	-RRB-	denotes	)
T17099	6339-6344	IN	denotes	After
T17100	6345-6351	NN	denotes	washes
T17101	6351-6352	-COMMA-	denotes	,
T17102	6353-6357	NN	denotes	cell
T17103	6358-6365	NN	denotes	pellets
T17104	6366-6370	VB	denotes	were
T17105	6371-6382	VB	denotes	resuspended
T17106	6383-6385	IN	denotes	at
T17107	6386-6388	CD	denotes	40
T17108	6389-6390	CC	denotes	×
T17109	6391-6394	CD	denotes	106
T17110	6395-6403	NN	denotes	cells/ml
T17111	6404-6406	IN	denotes	in
T17112	6407-6411	NN	denotes	ChIP
T17113	6412-6417	NN	denotes	lysis
T17114	6418-6424	NN	denotes	buffer
T17115	6425-6426	-LRB-	denotes	(
T17116	6426-6429	CD	denotes	150
T17117	6430-6432	NN	denotes	mM
T17118	6433-6437	NN	denotes	NaCl
T17119	6437-6438	-COMMA-	denotes	,
T17120	6439-6441	CD	denotes	25
T17121	6442-6444	NN	denotes	mM
T17122	6445-6449	NN	denotes	Tris
T17123	6450-6451	-LRB-	denotes	[
T17124	6451-6453	NN	denotes	pH
T17125	6454-6457	CD	denotes	7.5
T17126	6457-6458	-RRB-	denotes	]
T17127	6458-6459	-COMMA-	denotes	,
T17128	6460-6461	CD	denotes	1
T17129	6461-6462	NN	denotes	%
T17130	6463-6469	NN	denotes	Triton
T17131	6470-6475	NN	denotes	X-100
T17132	6475-6476	-COMMA-	denotes	,
T17133	6477-6480	CD	denotes	0.1
T17134	6480-6481	NN	denotes	%
T17135	6482-6485	NN	denotes	SDS
T17136	6485-6486	-COMMA-	denotes	,
T17137	6487-6490	CD	denotes	0.5
T17138	6490-6491	NN	denotes	%
T17139	6492-6504	NN	denotes	deoxycholate
T17140	6505-6509	CC	denotes	plus
T17141	6510-6518	NN	denotes	protease
T17142	6519-6522	CC	denotes	and
T17143	6523-6534	NN	denotes	phosphatase
T17144	6535-6545	NN	denotes	inhibitors
T17145	6545-6546	-RRB-	denotes	)
T17146	6546-6547	-COMMA-	denotes	,
T17147	6548-6551	CC	denotes	and
T17148	6552-6561	NN	denotes	chromatin
T17149	6562-6565	VB	denotes	was
T17150	6566-6573	VB	denotes	sheared
T17151	6574-6578	IN	denotes	with
T17152	6579-6580	DT	denotes	a
T17153	6581-6590	NN	denotes	sonicator
T17154	6591-6593	TO	denotes	to
T17155	6594-6599	VB	denotes	yield
T17156	6600-6608	JJ	denotes	0.5–1-kb
T17157	6609-6612	NN	denotes	DNA
T17158	6613-6622	NN	denotes	fragments
T17159	6624-6629	IN	denotes	After
T17160	6630-6641	VB	denotes	preclearing
T17161	6642-6645	DT	denotes	the
T17162	6646-6653	JJ	denotes	sheared
T17163	6654-6663	NN	denotes	chromatin
T17164	6664-6668	IN	denotes	with
T17165	6669-6676	NN	denotes	protein
T17166	6677-6688	NN	denotes	A–sepharose
T17167	6689-6694	NN	denotes	beads
T17168	6695-6698	CC	denotes	and
T17169	6699-6707	VB	denotes	removing
T17170	6708-6709	CD	denotes	5
T17171	6709-6710	NN	denotes	%
T17172	6711-6713	IN	denotes	as
T17173	6714-6719	NN	denotes	input
T17174	6720-6729	NN	denotes	chromatin
T17175	6729-6730	-COMMA-	denotes	,
T17176	6731-6750	NN	denotes	immunoprecipitation
T17177	6751-6754	VB	denotes	was
T17178	6755-6764	VB	denotes	performed
T17179	6765-6767	IN	denotes	by
T17180	6768-6774	VB	denotes	adding
T17181	6775-6784	VB	denotes	optimized
T17182	6785-6793	NN	denotes	antibody
T17183	6794-6801	NN	denotes	amounts
T17184	6802-6803	-LRB-	denotes	(
T17185	6803-6806	IN	denotes	per
T17186	6807-6809	CD	denotes	20
T17187	6810-6811	-COLON-	denotes	×
T17188	6812-6815	CD	denotes	106
T17189	6816-6820	NN	denotes	cell
T17190	6821-6832	NN	denotes	equivalents
T17191	6832-6833	-COLON-	denotes	:
T17192	6834-6837	CD	denotes	2.5
T17193	6838-6840	NN	denotes	μg
T17194	6841-6851	NN	denotes	anti-Eomes
T17195	6851-6852	-COMMA-	denotes	,
T17196	6853-6854	CD	denotes	1
T17197	6854-6855	SYM	denotes	:
T17198	6855-6858	CD	denotes	100
T17199	6859-6867	NN	denotes	dilution
T17200	6868-6878	NN	denotes	anti-Runx3
T17201	6878-6879	-RRB-	denotes	)
T17202	6879-6880	-COMMA-	denotes	,
T17203	6881-6889	VB	denotes	followed
T17204	6890-6892	IN	denotes	by
T17205	6893-6902	JJ	denotes	overnight
T17206	6903-6913	NN	denotes	incubation
T17207	6914-6916	IN	denotes	at
T17208	6917-6920	NN	denotes	4°C
T17209	6920-6921	-COLON-	denotes	;
T17210	6922-6929	NN	denotes	protein
T17211	6930-6941	NN	denotes	A–sepharose
T17212	6942-6947	NN	denotes	beads
T17213	6948-6952	VB	denotes	were
T17214	6953-6958	VB	denotes	added
T17215	6959-6962	IN	denotes	for
T17216	6963-6966	DT	denotes	the
T17217	6967-6971	JJ	denotes	last
T17218	6972-6973	CD	denotes	3
T17219	6974-6975	NN	denotes	h
T17220	6976-6978	IN	denotes	of
T17221	6979-6982	DT	denotes	the
T17222	6983-6993	NN	denotes	incubation
T17223	6994-7000	NN	denotes	period
T17224	7002-7007	NN	denotes	Beads
T17225	7008-7012	VB	denotes	were
T17226	7013-7019	VB	denotes	washed
T17227	7020-7022	CD	denotes	2×
T17228	7023-7027	IN	denotes	with
T17229	7028-7032	NN	denotes	RIPA
T17230	7033-7039	NN	denotes	buffer
T17231	7040-7041	-LRB-	denotes	(
T17232	7041-7043	CD	denotes	50
T17233	7044-7046	NN	denotes	mM
T17234	7047-7051	NN	denotes	Tris
T17235	7052-7053	-LRB-	denotes	[
T17236	7053-7055	NN	denotes	pH
T17237	7056-7057	CD	denotes	8
T17238	7057-7058	-RRB-	denotes	]
T17239	7058-7059	-COMMA-	denotes	,
T17240	7060-7063	CD	denotes	150
T17241	7064-7066	NN	denotes	mM
T17242	7067-7071	NN	denotes	NaCl
T17243	7071-7072	-COMMA-	denotes	,
T17244	7073-7074	CD	denotes	1
T17245	7075-7077	NN	denotes	mM
T17246	7078-7082	NN	denotes	EDTA
T17247	7082-7083	-COMMA-	denotes	,
T17248	7084-7085	CD	denotes	1
T17249	7085-7086	NN	denotes	%
T17250	7087-7092	NN	denotes	NP-40
T17251	7092-7093	-COMMA-	denotes	,
T17252	7094-7097	CD	denotes	0.1
T17253	7097-7098	NN	denotes	%
T17254	7099-7102	NN	denotes	SDS
T17255	7102-7103	-COMMA-	denotes	,
T17256	7104-7107	CD	denotes	0.5
T17257	7107-7108	NN	denotes	%
T17258	7109-7121	NN	denotes	deoxycholate
T17259	7121-7122	-RRB-	denotes	)
T17260	7122-7123	-COMMA-	denotes	,
T17261	7124-7126	CD	denotes	1×
T17262	7127-7131	IN	denotes	with
T17263	7132-7136	JJ	denotes	high
T17264	7137-7141	NN	denotes	salt
T17265	7142-7148	NN	denotes	buffer
T17266	7149-7150	-LRB-	denotes	(
T17267	7150-7152	CD	denotes	50
T17268	7153-7155	NN	denotes	mM
T17269	7156-7160	NN	denotes	Tris
T17270	7161-7162	-LRB-	denotes	[
T17271	7162-7164	NN	denotes	pH
T17272	7165-7166	CD	denotes	8
T17273	7166-7167	-RRB-	denotes	]
T17274	7167-7168	-COMMA-	denotes	,
T17275	7169-7172	CD	denotes	500
T17276	7173-7175	NN	denotes	mM
T17277	7176-7180	NN	denotes	NaCl
T17278	7180-7181	-COMMA-	denotes	,
T17279	7182-7183	CD	denotes	1
T17280	7184-7186	NN	denotes	mM
T17281	7187-7191	NN	denotes	EDTA
T17282	7191-7192	-COMMA-	denotes	,
T17283	7193-7194	CD	denotes	1
T17284	7194-7195	NN	denotes	%
T17285	7196-7201	NN	denotes	NP-40
T17286	7201-7202	-COMMA-	denotes	,
T17287	7203-7206	CD	denotes	0.1
T17288	7206-7207	NN	denotes	%
T17289	7208-7211	NN	denotes	SDS
T17290	7211-7212	-RRB-	denotes	)
T17291	7212-7213	-COMMA-	denotes	,
T17292	7214-7217	CC	denotes	and
T17293	7218-7220	NN	denotes	1×
T17294	7221-7225	IN	denotes	with
T17295	7226-7228	NN	denotes	TE
T17296	7229-7235	NN	denotes	buffer
T17297	7237-7242	IN	denotes	After
T17298	7243-7246	DT	denotes	the
T17299	7247-7251	JJ	denotes	last
T17300	7252-7256	NN	denotes	wash
T17301	7256-7257	-COMMA-	denotes	,
T17302	7258-7261	NN	denotes	DNA
T17303	7262-7265	VB	denotes	was
T17304	7266-7272	VB	denotes	eluted
T17305	7273-7275	IN	denotes	by
T17306	7276-7288	VB	denotes	resuspending
T17307	7289-7292	DT	denotes	the
T17308	7293-7298	NN	denotes	beads
T17309	7299-7301	IN	denotes	in
T17310	7302-7309	NN	denotes	elution
T17311	7310-7316	NN	denotes	buffer
T17312	7317-7318	-LRB-	denotes	(
T17313	7318-7319	CD	denotes	1
T17314	7319-7320	NN	denotes	%
T17315	7321-7324	NN	denotes	SDS
T17316	7324-7325	-COMMA-	denotes	,
T17317	7326-7329	CD	denotes	100
T17318	7330-7332	NN	denotes	mM
T17319	7333-7339	NN	denotes	NaHCO3
T17320	7339-7340	-RRB-	denotes	)
T17321	7342-7346	CC	denotes	Both
T17322	7347-7352	NN	denotes	input
T17323	7353-7356	CC	denotes	and
T17324	7357-7361	NN	denotes	ChIP
T17325	7362-7371	NN	denotes	chromatin
T17326	7372-7376	VB	denotes	were
T17327	7377-7381	RB	denotes	then
T17328	7382-7389	VB	denotes	treated
T17329	7390-7394	IN	denotes	with
T17330	7395-7400	NN	denotes	RNase
T17331	7401-7402	NN	denotes	A
T17332	7403-7404	-LRB-	denotes	(
T17333	7404-7405	CD	denotes	5
T17334	7406-7408	NN	denotes	μg
T17335	7409-7414	NN	denotes	total
T17336	7414-7415	-RRB-	denotes	)
T17337	7416-7419	IN	denotes	for
T17338	7420-7421	CD	denotes	1
T17339	7422-7423	NN	denotes	h
T17340	7424-7426	IN	denotes	at
T17341	7427-7431	NN	denotes	37°C
T17342	7431-7432	-COMMA-	denotes	,
T17343	7433-7441	VB	denotes	followed
T17344	7442-7444	IN	denotes	by
T17345	7445-7448	DT	denotes	the
T17346	7449-7457	NN	denotes	addition
T17347	7458-7460	IN	denotes	of
T17348	7461-7471	NN	denotes	proteinase
T17349	7472-7473	NN	denotes	K
T17350	7474-7475	-LRB-	denotes	(
T17351	7475-7478	CD	denotes	100
T17352	7479-7481	NN	denotes	μg
T17353	7482-7487	NN	denotes	total
T17354	7487-7488	-RRB-	denotes	)
T17355	7489-7492	CC	denotes	and
T17356	7493-7502	JJ	denotes	overnight
T17357	7503-7513	NN	denotes	incubation
T17358	7514-7516	IN	denotes	at
T17359	7517-7521	NN	denotes	65°C
T17360	7522-7524	TO	denotes	to
T17361	7525-7532	VB	denotes	reverse
T17362	7533-7546	NN	denotes	cross-linking
T17363	7548-7551	NN	denotes	DNA
T17364	7552-7555	VB	denotes	was
T17365	7556-7560	RB	denotes	then
T17366	7561-7569	VB	denotes	purified
T17367	7570-7574	IN	denotes	with
T17368	7575-7583	JJ	denotes	QIAquick
T17369	7584-7591	NN	denotes	columns
T17370	7592-7593	-LRB-	denotes	(
T17371	7593-7596	NN	denotes	Gel
T17372	7597-7607	NN	denotes	Extraction
T17373	7608-7611	NN	denotes	Kit
T17374	7611-7612	-COLON-	denotes	;
T17375	7613-7619	NN	denotes	QIAGEN
T17376	7619-7620	-RRB-	denotes	)
T17377	7621-7630	VB	denotes	according
T17378	7631-7633	TO	denotes	to
T17379	7634-7637	DT	denotes	the
T17380	7638-7650	NN	denotes	manufacturer
T17381	7650-7652	POS	denotes	's
T17382	7653-7665	NN	denotes	instructions
T17383	7666-7669	CC	denotes	and
T17384	7670-7681	VB	denotes	resuspended
T17385	7682-7684	IN	denotes	in
T17386	7685-7686	DT	denotes	a
T17387	7687-7692	JJ	denotes	50-μl
T17388	7693-7699	NN	denotes	volume
T17389	7701-7704	IN	denotes	For
T17390	7705-7714	JJ	denotes	real-time
T17391	7715-7718	NN	denotes	PCR
T17392	7719-7728	NN	denotes	detection
T17393	7729-7731	IN	denotes	of
T17394	7732-7750	JJ	denotes	immunoprecipitated
T17395	7751-7758	NN	denotes	targets
T17396	7759-7764	VB	denotes	using
T17397	7765-7768	DT	denotes	the
T17398	7769-7773	NNP	denotes	SYBR
T17399	7774-7779	NNP	denotes	Green
T17400	7780-7783	NN	denotes	PCR
T17401	7784-7787	NN	denotes	Kit
T17402	7787-7788	-COMMA-	denotes	,
T17403	7789-7790	DT	denotes	a
T17404	7791-7799	JJ	denotes	standard
T17405	7800-7805	NN	denotes	curve
T17406	7806-7809	VB	denotes	was
T17407	7810-7818	VB	denotes	obtained
T17408	7819-7823	IN	denotes	with
T17409	7824-7830	JJ	denotes	serial
T17410	7831-7840	NN	denotes	dilutions
T17411	7841-7843	IN	denotes	of
T17412	7844-7849	NN	denotes	input
T17413	7850-7853	NN	denotes	DNA
T17414	7854-7857	IN	denotes	for
T17415	7858-7862	DT	denotes	each
T17416	7863-7869	NN	denotes	sample
T17417	7869-7870	-COMMA-	denotes	,
T17418	7871-7874	CC	denotes	and
T17419	7875-7876	CD	denotes	1
T17420	7877-7879	NN	denotes	μl
T17421	7880-7884	NN	denotes	ChIP
T17422	7885-7888	NN	denotes	DNA
T17423	7889-7892	VB	denotes	was
T17424	7893-7897	VB	denotes	used
T17425	7898-7901	IN	denotes	per
T17426	7902-7905	NN	denotes	PCR
T17427	7906-7914	NN	denotes	reaction
T17428	7915-7916	-LRB-	denotes	(
T17429	7916-7925	VB	denotes	performed
T17430	7926-7928	IN	denotes	in
T17431	7929-7939	NN	denotes	duplicates
T17432	7939-7940	-RRB-	denotes	)
T17433	7942-7946	NN	denotes	Melt
T17434	7947-7953	NN	denotes	curves
T17435	7954-7957	CC	denotes	and
T17436	7958-7965	NN	denotes	agarose
T17437	7966-7970	NN	denotes	gels
T17438	7971-7975	VB	denotes	were
T17439	7976-7984	VB	denotes	analyzed
T17440	7985-7987	TO	denotes	to
T17441	7988-7994	VB	denotes	ensure
T17442	7995-8008	NN	denotes	amplification
T17443	8009-8011	IN	denotes	of
T17444	8012-8020	JJ	denotes	specific
T17445	8021-8027	NN	denotes	target
T17446	8028-8037	NN	denotes	sequences
T17447	8039-8044	VB	denotes	Refer
T17448	8045-8047	TO	denotes	to
T17449	8048-8053	JJ	denotes	Table
T17450	8054-8056	NN	denotes	S1
T17451	8057-8058	-LRB-	denotes	(
T17452	8058-8067	JJ	denotes	available
T17453	8068-8070	IN	denotes	at
T17454	8071-8075	NN	denotes	http
T17455	8075-8076	-COLON-	denotes	:
T17456	8076-8123	NN	denotes	//www.jem.org/cgi/content/full/jem.20081242/DC1
T17457	8123-8124	-RRB-	denotes	)
T17458	8125-8128	IN	denotes	for
T17459	8129-8130	DT	denotes	a
T17460	8131-8135	NN	denotes	list
T17461	8136-8138	IN	denotes	of
T17462	8139-8145	NN	denotes	primer
T17463	8146-8150	NN	denotes	sets
T17464	8152-8155	DT	denotes	The
T17465	8156-8160	NN	denotes	data
T17466	8161-8164	VB	denotes	are
T17467	8165-8174	VB	denotes	presented
T17468	8175-8177	IN	denotes	as
T17469	8178-8181	DT	denotes	the
T17470	8182-8188	NN	denotes	number
T17471	8189-8191	IN	denotes	of
T17472	8192-8210	VB	denotes	immunoprecipitated
T17473	8211-8217	NN	denotes	target
T17474	8218-8227	NN	denotes	sequences
T17475	8228-8236	JJ	denotes	relative
T17476	8237-8239	TO	denotes	to
T17477	8240-8245	NN	denotes	input
T17478	8246-8255	NN	denotes	chromatin
T17479	8255-8256	-COMMA-	denotes	,
T17480	8257-8265	VB	denotes	assuming
T17481	8266-8269	CD	denotes	two
T17482	8270-8276	NN	denotes	copies
T17483	8277-8279	IN	denotes	of
T17484	8280-8286	NN	denotes	target
T17485	8287-8295	NN	denotes	sequence
T17486	8296-8299	IN	denotes	per
T17487	8300-8304	NN	denotes	cell
T17488	8305-8315	JJ	denotes	equivalent
T17489	8316-8320	VB	denotes	used
T17490	8321-8324	IN	denotes	for
T17491	8325-8328	DT	denotes	the
T17492	8329-8333	NN	denotes	ChIP
T17850	8465-8466	-COMMA-	denotes	,
T17851	8467-8469	CD	denotes	10
T17852	8470-8472	NN	denotes	μg
T17853	8473-8475	IN	denotes	of
T17854	8476-8481	JJ	denotes	total
T17855	8482-8485	NN	denotes	RNA
T17856	8486-8489	VB	denotes	was
T17857	8490-8496	VB	denotes	loaded
T17858	8497-8500	IN	denotes	per
T17859	8501-8505	NN	denotes	lane
T17860	8506-8509	CC	denotes	and
T17861	8510-8521	VB	denotes	transferred
T17862	8522-8524	TO	denotes	to
T17863	8525-8535	RB	denotes	positively
T17864	8536-8543	VB	denotes	charged
T17865	8544-8549	NN	denotes	nylon
T17866	8550-8559	NN	denotes	membranes
T17867	8560-8561	-LRB-	denotes	(
T17868	8561-8570	JJ	denotes	Hybond-N+
T17869	8570-8571	-COLON-	denotes	;
T17870	8572-8574	NNP	denotes	GE
T17871	8575-8585	NNP	denotes	Healthcare
T17872	8585-8586	-RRB-	denotes	)
T17873	8586-8587	-COMMA-	denotes	,
T17874	8588-8593	WDT	denotes	which
T17875	8594-8597	VB	denotes	was
T17876	8598-8607	VB	denotes	confirmed
T17877	8608-8610	IN	denotes	by
T17878	8611-8619	NN	denotes	ethidium
T17879	8620-8627	NN	denotes	bromide
T17880	8628-8636	NN	denotes	staining
T17881	8637-8639	IN	denotes	of
T17882	8640-8649	JJ	denotes	ribosomal
T17883	8650-8653	NN	denotes	RNA
T17884	8654-8661	NN	denotes	species
T17885	8662-8664	IN	denotes	on
T17886	8665-8668	DT	denotes	the
T17887	8669-8677	NN	denotes	membrane
T17888	8679-8688	NN	denotes	Membranes
T17889	8689-8693	VB	denotes	were
T17890	8694-8704	VB	denotes	hybridized
T17891	8705-8709	IN	denotes	with
T17892	8710-8711	CD	denotes	1
T17893	8712-8717	NN	denotes	ng/ml
T17894	8718-8720	SYM	denotes	α-
T17895	8720-8721	-LRB-	denotes	[
T17896	8721-8724	NN	denotes	32P
T17897	8724-8725	-RRB-	denotes	]
T17898	8725-8737	VB	denotes	dCTP–labeled
T17899	8738-8753	JJ	denotes	trichloroacetic
T17900	8754-8758	NN	denotes	acid
T17901	8759-8771	JJ	denotes	precipitable
T17902	8772-8777	NN	denotes	probe
T17903	8778-8780	IN	denotes	in
T17904	8781-8791	NN	denotes	ExpressHyb
T17905	8792-8805	NN	denotes	hybridization
T17906	8806-8812	NN	denotes	buffer
T17907	8813-8814	-LRB-	denotes	(
T17908	8814-8822	NNP	denotes	Clontech
T17909	8823-8835	NNP	denotes	Laboratories
T17910	8835-8836	-COMMA-	denotes	,
T17911	8837-8841	NNP	denotes	Inc.
T17912	8841-8842	-RRB-	denotes	)
T17913	8844-8847	DT	denotes	All
T17914	8848-8852	NN	denotes	cDNA
T17915	8853-8859	NN	denotes	probes
T17916	8860-8864	VB	denotes	were
T17917	8865-8874	VB	denotes	confirmed
T17918	8875-8877	TO	denotes	to
T17919	8878-8882	VB	denotes	have
T17920	8883-8886	DT	denotes	the
T17921	8887-8898	JJ	denotes	appropriate
T17922	8899-8910	JJ	denotes	single-copy
T17923	8911-8922	NN	denotes	specificity
T17924	8923-8928	IN	denotes	under
T17925	8929-8934	DT	denotes	these
T17926	8935-8945	NN	denotes	conditions
T17927	8946-8951	VB	denotes	using
T17928	8952-8959	JJ	denotes	genomic
T17929	8960-8968	JJ	denotes	Southern
T17930	8969-8973	NN	denotes	blot
T17931	8974-8982	NN	denotes	analysis
T17932	8984-8988	NN	denotes	Band
T17933	8989-9000	NN	denotes	intensities
T17934	9001-9005	VB	denotes	were
T17935	9006-9014	VB	denotes	acquired
T17936	9015-9017	IN	denotes	by
T17937	9018-9033	VB	denotes	phosphorimaging
T17938	9034-9042	NN	denotes	analysis
T17939	9044-9047	IN	denotes	For
T17940	9048-9055	JJ	denotes	Western
T17941	9056-9064	NN	denotes	analysis
T17942	9064-9065	-COMMA-	denotes	,
T17943	9066-9076	JJ	denotes	whole-cell
T17944	9077-9084	NN	denotes	protein
T17945	9085-9092	NN	denotes	lysates
T17946	9093-9097	VB	denotes	were
T17947	9098-9106	VB	denotes	obtained
T17948	9107-9111	IN	denotes	from
T17949	9112-9116	JJ	denotes	CD8+
T17950	9117-9118	NN	denotes	T
T17951	9119-9124	NN	denotes	cells
T17952	9125-9127	IN	denotes	at
T17953	9128-9131	DT	denotes	the
T17954	9132-9136	NN	denotes	time
T17955	9137-9143	NN	denotes	points
T17956	9144-9153	VB	denotes	indicated
T17957	9154-9156	IN	denotes	in
T17958	9157-9160	DT	denotes	the
T17959	9161-9168	NN	denotes	figures
T17960	9169-9175	IN	denotes	during
T17961	9176-9182	JJ	denotes	clonal
T17962	9183-9192	NN	denotes	expansion
T17963	9193-9195	IN	denotes	in
T17964	9196-9199	CD	denotes	100
T17965	9200-9204	NN	denotes	U/ml
T17966	9205-9209	NN	denotes	IL-2
T17967	9210-9214	IN	denotes	with
T17968	9215-9220	NN	denotes	lysis
T17969	9221-9227	NN	denotes	buffer
T17970	9228-9229	-LRB-	denotes	(
T17971	9229-9231	CD	denotes	50
T17972	9232-9234	NN	denotes	mM
T17973	9235-9239	NN	denotes	Tris
T17974	9240-9241	-LRB-	denotes	[
T17975	9241-9243	NN	denotes	pH
T17976	9244-9247	CD	denotes	7.5
T17977	9247-9248	-RRB-	denotes	]
T17978	9248-9249	-COMMA-	denotes	,
T17979	9250-9253	CD	denotes	150
T17980	9254-9256	NN	denotes	mM
T17981	9257-9261	NN	denotes	NaCl
T17982	9261-9262	-COMMA-	denotes	,
T17983	9263-9265	CD	denotes	10
T17984	9265-9266	NN	denotes	%
T17985	9267-9275	NN	denotes	glycerol
T17986	9275-9276	-COMMA-	denotes	,
T17987	9277-9278	CD	denotes	5
T17988	9279-9281	NN	denotes	mM
T17989	9282-9286	NN	denotes	EDTA
T17990	9286-9287	-COMMA-	denotes	,
T17991	9288-9289	CD	denotes	1
T17992	9289-9290	NN	denotes	%
T17993	9291-9296	NN	denotes	NP-40
T17994	9296-9297	-RRB-	denotes	)
T17995	9298-9300	IN	denotes	by
T17996	9301-9313	VB	denotes	resuspending
T17997	9314-9321	NN	denotes	samples
T17998	9322-9324	IN	denotes	in
T17999	9325-9327	CD	denotes	10
T18000	9328-9330	NN	denotes	μl
T18001	9331-9334	IN	denotes	per
T18002	9335-9338	CD	denotes	106
T18003	9339-9344	NN	denotes	cells
T18004	9345-9348	CC	denotes	and
T18005	9349-9359	VB	denotes	incubating
T18006	9360-9362	IN	denotes	on
T18007	9363-9366	NN	denotes	ice
T18008	9367-9370	IN	denotes	for
T18009	9371-9373	CD	denotes	30
T18010	9374-9377	NN	denotes	min
T18011	9378-9380	IN	denotes	in
T18012	9381-9384	DT	denotes	the
T18013	9385-9393	NN	denotes	presence
T18014	9394-9396	IN	denotes	of
T18015	9397-9405	NN	denotes	protease
T18016	9406-9416	NN	denotes	inhibitors
T18017	9418-9428	NN	denotes	Immunoblot
T18018	9429-9437	NN	denotes	analysis
T18019	9438-9441	VB	denotes	was
T18020	9442-9451	VB	denotes	performed
T18021	9452-9456	IN	denotes	with
T18022	9457-9460	DT	denotes	the
T18023	9461-9471	NN	denotes	antibodies
T18024	9472-9481	VB	denotes	indicated
T18025	9482-9484	IN	denotes	in
T18026	9485-9488	DT	denotes	the
T18027	9489-9496	NN	denotes	figures
T18028	9497-9502	IN	denotes	after
T18029	9503-9511	NN	denotes	SDS-PAGE
T18030	9512-9513	-LRB-	denotes	(
T18031	9513-9518	CD	denotes	10–30
T18032	9519-9521	NN	denotes	μg
T18033	9522-9524	IN	denotes	of
T18034	9525-9530	JJ	denotes	total
T18035	9531-9538	NN	denotes	protein
T18036	9539-9542	VB	denotes	was
T18037	9543-9549	VB	denotes	loaded
T18038	9550-9553	IN	denotes	per
T18039	9554-9558	RB	denotes	well
T18040	9558-9559	-RRB-	denotes	)
T18041	9561-9575	NN	denotes	Quantification
T18042	9576-9578	IN	denotes	of
T18043	9579-9587	VB	denotes	detected
T18044	9588-9595	NN	denotes	protein
T18045	9596-9599	VB	denotes	was
T18046	9600-9609	VB	denotes	performed
T18047	9610-9614	IN	denotes	with
T18048	9615-9617	DT	denotes	an
T18049	9618-9629	NNP	denotes	Intelligent
T18050	9630-9634	NNP	denotes	Dark
T18051	9635-9638	NNP	denotes	Box
T18052	9639-9643	NN	denotes	unit
T18053	9644-9645	-LRB-	denotes	(
T18054	9645-9653	NN	denotes	LAS-3000
T18055	9653-9654	-COLON-	denotes	;
T18056	9655-9663	NNP	denotes	Fujifilm
T18057	9663-9664	-RRB-	denotes	)
T18058	9665-9668	CC	denotes	and
T18059	9669-9679	VB	denotes	normalized
T18060	9680-9683	IN	denotes	for
T18061	9684-9691	VB	denotes	loading
T18062	9692-9696	IN	denotes	with
T18063	9697-9700	DT	denotes	the
T18064	9701-9707	NN	denotes	amount
T18065	9708-9710	IN	denotes	of
T18066	9711-9714	NN	denotes	RNA
T18067	9715-9721	NN	denotes	Pol-II
T18068	9722-9730	VB	denotes	detected
T18069	9731-9733	IN	denotes	in
T18070	9734-9738	DT	denotes	each
T18071	9739-9743	NN	denotes	lane
T18190	9746-9752	NN	denotes	Online
T18191	9753-9765	JJ	denotes	supplemental
T18192	9766-9774	NN	denotes	material
T18193	9776-9780	NN	denotes	Fig.
T18194	9781-9783	NN	denotes	S1
T18195	9784-9789	VB	denotes	shows
T18196	9790-9793	DT	denotes	the
T18197	9794-9810	NN	denotes	characterization
T18198	9811-9813	IN	denotes	of
T18199	9814-9824	JJ	denotes	peripheral
T18200	9825-9829	JJ	denotes	CD8+
T18201	9830-9831	NN	denotes	T
T18202	9832-9837	NN	denotes	cells
T18203	9838-9842	IN	denotes	from
T18204	9843-9851	NN	denotes	Runx3−/−
T18205	9852-9856	NN	denotes	mice
T18206	9858-9862	NN	denotes	Fig.
T18207	9863-9865	NN	denotes	S2
T18208	9866-9871	VB	denotes	shows
T18209	9872-9880	NN	denotes	effector
T18210	9881-9888	NN	denotes	protein
T18211	9889-9899	NN	denotes	expression
T18212	9900-9902	IN	denotes	by
T18213	9903-9908	NN	denotes	Runx3
T18214	9909-9911	NN	denotes	WT
T18215	9912-9915	CC	denotes	and
T18216	9916-9918	NN	denotes	KO
T18217	9919-9924	NN	denotes	cells
T18218	9925-9927	IN	denotes	at
T18219	9928-9931	NN	denotes	day
T18220	9932-9933	CD	denotes	4
T18221	9934-9936	IN	denotes	of
T18222	9937-9939	FW	denotes	in
T18223	9940-9945	FW	denotes	vitro
T18224	9946-9953	NN	denotes	culture
T18225	9955-9961	NN	denotes	Primer
T18226	9962-9971	NN	denotes	sequences
T18227	9972-9976	VB	denotes	used
T18228	9977-9980	IN	denotes	for
T18229	9981-9985	NN	denotes	ChIP
T18230	9986-9997	NN	denotes	experiments
T18231	9998-10001	VB	denotes	are
T18232	10002-10007	VB	denotes	shown
T18233	10008-10010	IN	denotes	in
T18234	10011-10016	JJ	denotes	Table
T18235	10017-10019	NN	denotes	S1
T18236	10021-10027	NN	denotes	Online
T18237	10028-10040	JJ	denotes	supplemental
T18238	10041-10049	NN	denotes	material
T18239	10050-10052	VB	denotes	is
T18240	10053-10062	JJ	denotes	available
T18241	10063-10065	IN	denotes	at
T18242	10066-10070	NN	denotes	http
T18243	10070-10071	-COLON-	denotes	:
T18244	10071-10118	NN	denotes	//www.jem.org/cgi/content/full/jem.20081242/DC1
T17829	8336-8344	JJ	denotes	Northern
T17830	8345-8348	CC	denotes	and
T17831	8349-8356	JJ	denotes	Western
T17832	8357-8361	NN	denotes	blot
T17833	8362-8370	NN	denotes	analyses
T17834	8372-8375	NN	denotes	RNA
T17835	8376-8385	NN	denotes	isolation
T17836	8386-8389	CC	denotes	and
T17837	8390-8398	JJ	denotes	Northern
T17838	8399-8403	NN	denotes	blot
T17839	8404-8412	NN	denotes	analysis
T17840	8413-8416	VB	denotes	was
T17841	8417-8426	VB	denotes	performed
T17842	8427-8429	IN	denotes	as
T17843	8430-8440	RB	denotes	previously
T17844	8441-8450	VB	denotes	described
T17845	8451-8452	-LRB-	denotes	(
T17846	8452-8454	CD	denotes	29
T17847	8454-8455	-RRB-	denotes	)
T17848	8457-8459	IN	denotes	In
T17849	8460-8465	NN	denotes	brief
T16598	4888-4898	RB	denotes	previously
T16599	4899-4908	VB	denotes	optimized
T16600	4909-4923	NN	denotes	concentrations
T16601	4923-4924	-COMMA-	denotes	,
T16602	4925-4934	VB	denotes	incubated
T16603	4935-4938	IN	denotes	for
T16604	4939-4941	CD	denotes	15
T16605	4942-4945	NN	denotes	min
T16606	4946-4948	IN	denotes	at
T16607	4949-4953	NN	denotes	room
T16608	4954-4965	NN	denotes	temperature
R10973	T14019	T14020	arg1Of	Antibodies,and
R10974	T14021	T14020	arg2Of	reagents,and
R10975	T14024	T14022	arg1Of	antibodies,The
R10976	T14024	T14023	arg1Of	antibodies,following
R10977	T14024	T14025	arg2Of	antibodies,used
R10978	T14024	T14031	arg1Of	antibodies,were
R10979	T14024	T14032	arg2Of	antibodies,obtained
R10980	T14025	T14026	arg1Of	used,for
R10981	T14030	T14026	arg2Of	stains,for
R10982	T14030	T14027	arg1Of	stains,intracellular
R10983	T14030	T14028	arg1Of	stains,or
R10984	T14030	T14029	arg1Of	stains,surface
R10985	T14032	T14031	arg2Of	obtained,were
R10986	T14032	T14033	arg1Of	obtained,from
R10987	T14032	T14035	arg1Of	obtained,:
R10988	T14032	T14036	arg1Of	obtained,anti–IL-2
R10989	T14034	T14033	arg2Of	eBioscience,from
R10990	T14036	T14037	arg1Of	anti–IL-2,","
R10991	T14038	T14039	arg1Of	anti–IFN-γ,","
R10992	T14039	T14041	arg1Of	",",","
R10993	T14040	T14039	arg2Of	anti-TNF,","
R10994	T14042	T14041	arg2Of	anti–granzyme,","
R10995	T14043	T14044	arg1Of	B,","
R10996	T14044	T14046	arg1Of	",",","
R10997	T14045	T14044	arg2Of	anti-CD8,","
R10998	T14046	T14049	arg1Of	",",and
R10999	T14047	T14046	arg2Of	anti-CD25,","
R11000	T14049	T14037	arg2Of	and,","
R11001	T14049	T14038	arg1Of	and,anti–IFN-γ
R11002	T14049	T14040	arg1Of	and,anti-TNF
R11003	T14049	T14042	arg1Of	and,anti–granzyme
R11004	T14049	T14048	arg1Of	and,","
R11005	T14050	T14049	arg2Of	anti-CD44,and
R11006	T14051	T14052	arg1Of	Anti-CD69,was
R11007	T14051	T14053	arg2Of	Anti-CD69,purchased
R11008	T14053	T14052	arg2Of	purchased,was
R11009	T14053	T14054	arg1Of	purchased,from
R11010	T14055	T14054	arg2Of	BD,from
R11011	T14058	T14056	arg2Of	experiments,For
R11012	T14058	T14057	arg1Of	experiments,ChIP
R11013	T14062	T14060	arg1Of	antibody,the
R11014	T14062	T14061	arg1Of	antibody,anti-Eomes
R11015	T14062	T14063	arg1Of	antibody,was
R11016	T14062	T14064	arg2Of	antibody,obtained
R11017	T14064	T14063	arg2Of	obtained,was
R11018	T14064	T14065	arg1Of	obtained,from
R11019	T14064	T14067	arg1Of	obtained,and
R11020	T14066	T14065	arg2Of	Abcam,from
R11021	T14067	T14056	arg1Of	and,For
R11022	T14067	T14059	arg1Of	and,","
R11023	T14070	T14068	arg1Of	antibody,the
R11024	T14070	T14069	arg1Of	antibody,anti-Runx3
R11025	T14070	T14071	arg1Of	antibody,was
R11026	T14070	T14072	arg2Of	antibody,produced
R11027	T14072	T14067	arg2Of	produced,and
R11028	T14072	T14071	arg2Of	produced,was
R11029	T14076	T14072	arg1Of	laboratory,produced
R11030	T14076	T14073	arg2Of	laboratory,by
R11031	T14076	T14074	arg1Of	laboratory,the
R11032	T14076	T14075	arg1Of	laboratory,Groner
R11033	T14079	T14077	arg1Of	antibodies,The
R11034	T14079	T14078	arg1Of	antibodies,following
R11035	T14079	T14080	arg1Of	antibodies,were
R11036	T14079	T14081	arg2Of	antibodies,used
R11037	T14081	T14080	arg2Of	used,were
R11038	T14081	T14082	arg1Of	used,for
R11039	T14083	T14082	arg2Of	immunoblotting,for
R11040	T14083	T14084	arg1Of	immunoblotting,:
R11041	T14085	T14086	arg1Of	antiperforin,(
R11042	T14085	T14089	arg1Of	antiperforin,","
R11043	T14087	T14086	arg2Of	Abcam,(
R11044	T14088	T14086	arg3Of	),(
R11045	T14089	T14095	arg1Of	",",and
R11046	T14090	T14089	arg2Of	anti-Eomes,","
R11047	T14090	T14091	arg1Of	anti-Eomes,(
R11048	T14092	T14091	arg2Of	Abcam,(
R11049	T14093	T14091	arg3Of	),(
R11050	T14095	T14084	arg2Of	and,:
R11051	T14095	T14094	arg1Of	and,","
R11052	T14096	T14095	arg2Of	anti–Pol-II,and
R11053	T14096	T14097	arg1Of	anti–Pol-II,(
R11054	T14100	T14097	arg2Of	Biotechnology,(
R11055	T14100	T14098	arg1Of	Biotechnology,Santa
R11056	T14100	T14099	arg1Of	Biotechnology,Cruz
R11057	T14100	T14101	arg1Of	Biotechnology,","
R11058	T14102	T14101	arg2Of	Inc.,","
R11059	T14103	T14097	arg3Of	),(
R11060	T14106	T14104	arg1Of	antibody,The
R11061	T14106	T14105	arg1Of	antibody,T-bet
R11062	T14106	T14107	arg1Of	antibody,was
R11063	T14106	T14108	arg2Of	antibody,provided
R11064	T14108	T14107	arg2Of	provided,was
R11065	T14111	T14108	arg1Of	Glimcher,provided
R11066	T14111	T14109	arg2Of	Glimcher,by
R11067	T14111	T14110	arg1Of	Glimcher,L.
R11068	T14111	T14112	arg1Of	Glimcher,(
R11069	T14114	T14113	arg1Of	School,Harvard
R11070	T14114	T14115	arg1Of	School,of
R11071	T14114	T14118	arg1Of	School,","
R11072	T14117	T14115	arg2Of	Health,of
R11073	T14117	T14116	arg1Of	Health,Public
R11074	T14118	T14112	arg2Of	",",(
R11075	T14118	T14120	arg1Of	",",","
R11076	T14119	T14118	arg2Of	Boston,","
R11077	T14121	T14120	arg2Of	MA,","
R11078	T14122	T14112	arg3Of	),(
R11079	T14125	T14123	arg1Of	reagents,The
R11080	T14125	T14124	arg1Of	reagents,following
R11081	T14125	T14126	arg1Of	reagents,were
R11082	T14125	T14127	arg2Of	reagents,used
R11083	T14127	T14126	arg2Of	used,were
R11084	T14127	T14128	arg1Of	used,for
R11085	T14127	T14135	arg1Of	used,:
R11086	T14130	T14128	arg2Of	experiments,for
R11087	T14130	T14129	arg1Of	experiments,the
R11088	T14130	T14131	arg2Of	experiments,presented
R11089	T14131	T14132	arg1Of	presented,in
R11090	T14134	T14132	arg2Of	report,in
R11091	T14134	T14133	arg1Of	report,this
R11092	T14138	T14137	arg1Of	Apoptosis,V–FITC
R11093	T14140	T14136	arg1Of	Kit,Annexin
R11094	T14140	T14138	arg1Of	Kit,Apoptosis
R11095	T14140	T14139	arg1Of	Kit,Detection
R11096	T14140	T14141	arg1Of	Kit,(
R11097	T14140	T14144	arg1Of	Kit,","
R11098	T14142	T14141	arg2Of	BD,(
R11099	T14143	T14141	arg3Of	),(
R11100	T14145	T14144	arg2Of	CD8,","
R11101	T14148	T14140	arg1Of	Kit,Kit
R11102	T14148	T14145	arg1Of	Kit,CD8
R11103	T14148	T14146	arg1Of	Kit,Negative
R11104	T14148	T14147	arg1Of	Kit,Isolation
R11105	T14148	T14149	arg1Of	Kit,(
R11106	T14148	T14152	arg1Of	Kit,","
R11107	T14150	T14149	arg2Of	Invitrogen,(
R11108	T14151	T14149	arg3Of	),(
R11109	T14152	T14160	arg1Of	",",and
R11110	T14154	T14152	arg2Of	MicroBeads,","
R11111	T14154	T14153	arg1Of	MicroBeads,CD8
R11112	T14154	T14155	arg1Of	MicroBeads,(
R11113	T14157	T14155	arg2Of	Biotec,(
R11114	T14157	T14156	arg1Of	Biotec,Miltenyi
R11115	T14158	T14155	arg3Of	),(
R11116	T14160	T14159	arg1Of	and,","
R11117	T14165	T14160	arg2Of	Reagents,and
R11118	T14165	T14161	arg1Of	Reagents,SYBR
R11119	T14165	T14162	arg1Of	Reagents,Green
R11120	T14165	T14163	arg1Of	Reagents,PCR
R11121	T14165	T14164	arg1Of	Reagents,Core
R11122	T14165	T14166	arg1Of	Reagents,(
R11123	T14168	T14166	arg2Of	Biosystems,(
R11124	T14168	T14167	arg1Of	Biosystems,Applied
R11125	T14169	T14166	arg3Of	),(
R11126	T14172	T14170	arg1Of	peptide,The
R11127	T14172	T14171	arg1Of	peptide,Gp33
R11128	T14172	T14173	arg1Of	peptide,(
R11129	T14172	T14176	arg1Of	peptide,was
R11130	T14172	T14177	arg2Of	peptide,synthesized
R11131	T14174	T14173	arg2Of	KAVYNFATC,(
R11132	T14175	T14173	arg3Of	),(
R11133	T14177	T14176	arg2Of	synthesized,was
R11134	T14177	T14185	arg1Of	synthesized,and
R11135	T14183	T14177	arg1Of	Facility,synthesized
R11136	T14183	T14178	arg2Of	Facility,by
R11137	T14183	T14179	arg1Of	Facility,the
R11138	T14183	T14180	arg1Of	Facility,Tufts
R11139	T14183	T14181	arg1Of	Facility,University
R11140	T14183	T14182	arg1Of	Facility,Core
R11141	T14185	T14184	arg1Of	and,","
R11142	T14187	T14186	arg1Of	mM,10
R11143	T14187	T14188	arg1Of	mM,of
R11144	T14187	T14191	arg1Of	mM,was
R11145	T14187	T14192	arg2Of	mM,prepared
R11146	T14190	T14188	arg2Of	solutions,of
R11147	T14190	T14189	arg1Of	solutions,stock
R11148	T14192	T14185	arg2Of	prepared,and
R11149	T14192	T14191	arg2Of	prepared,was
R11150	T14192	T14193	arg1Of	prepared,in
R11151	T14194	T14193	arg2Of	DMSO,in
R11380	T14607	T14608	arg1Of	Isolation,and
R11381	T14608	T14610	arg1Of	and,of
R11382	T14609	T14608	arg2Of	culture,and
R11383	T14614	T14610	arg2Of	cells,of
R11384	T14614	T14611	arg1Of	cells,primary
R11385	T14614	T14612	arg1Of	cells,CD8+
R11386	T14614	T14613	arg1Of	cells,T
R11387	T14617	T14615	arg1Of	cells,CD8+
R11388	T14617	T14616	arg1Of	cells,T
R11389	T14617	T14618	arg1Of	cells,from
R11390	T14617	T14624	arg1Of	cells,transgenic
R11391	T14617	T14628	arg1Of	cells,","
R11392	T14623	T14618	arg2Of	TCR,from
R11393	T14623	T14619	arg1Of	TCR,4–8-wk-old
R11394	T14623	T14620	arg1Of	TCR,Tcra−/−
R11395	T14623	T14621	arg1Of	TCR,×
R11396	T14623	T14622	arg1Of	TCR,P14
R11397	T14624	T14625	arg1Of	transgenic,(
R11398	T14626	T14625	arg2Of	Taconic,(
R11399	T14627	T14625	arg3Of	),(
R11400	T14628	T14632	arg1Of	",",or
R11401	T14630	T14628	arg2Of	WT,","
R11402	T14630	T14629	arg1Of	WT,C57BL/6J
R11403	T14632	T14631	arg1Of	or,","
R11404	T14632	T14640	arg1Of	or,were
R11405	T14632	T14641	arg2Of	or,purified
R11406	T14633	T14634	arg1Of	Tbx21−/−,(
R11407	T14637	T14634	arg2Of	Laboratory,(
R11408	T14637	T14635	arg1Of	Laboratory,The
R11409	T14637	T14636	arg1Of	Laboratory,Jackson
R11410	T14638	T14634	arg3Of	),(
R11411	T14639	T14632	arg2Of	mice,or
R11412	T14639	T14633	arg1Of	mice,Tbx21−/−
R11413	T14641	T14640	arg2Of	purified,were
R11414	T14641	T14642	arg1Of	purified,(
R11415	T14641	T14648	arg1Of	purified,by
R11416	T14641	T14654	arg1Of	purified,from
R11417	T14644	T14643	arg1Of	95,>
R11418	T14644	T14645	arg1Of	95,%
R11419	T14646	T14642	arg2Of	purity,(
R11420	T14646	T14644	arg1Of	purity,95
R11421	T14647	T14642	arg3Of	),(
R11422	T14650	T14648	arg2Of	selection,by
R11423	T14650	T14649	arg1Of	selection,negative
R11424	T14650	T14651	arg1Of	selection,(
R11425	T14652	T14651	arg2Of	Invitrogen,(
R11426	T14653	T14651	arg3Of	),(
R11427	T14656	T14657	arg1Of	spleen,and
R11428	T14658	T14657	arg2Of	lymph,and
R11429	T14660	T14654	arg2Of	cells,from
R11430	T14660	T14655	arg2Of	cells,pooled
R11431	T14660	T14656	arg1Of	cells,spleen
R11432	T14660	T14658	arg1Of	cells,lymph
R11433	T14660	T14659	arg1Of	cells,node
R11434	T14663	T14661	arg1Of	cells,CD8+
R11435	T14663	T14662	arg1Of	cells,T
R11436	T14663	T14664	arg1Of	cells,from
R11437	T14663	T14671	arg1Of	cells,were
R11438	T14663	T14672	arg2Of	cells,purified
R11439	T14666	T14664	arg2Of	mice,from
R11440	T14666	T14665	arg1Of	mice,Runx3−/−
R11441	T14666	T14667	arg1Of	mice,on
R11442	T14670	T14667	arg2Of	background,on
R11443	T14670	T14668	arg1Of	background,the
R11444	T14670	T14669	arg1Of	background,ICR
R11445	T14672	T14671	arg2Of	purified,were
R11446	T14675	T14672	arg1Of	selection,purified
R11447	T14675	T14673	arg2Of	selection,by
R11448	T14675	T14674	arg1Of	selection,positive
R11449	T14675	T14676	arg1Of	selection,(
R11450	T14678	T14676	arg2Of	Biotec,(
R11451	T14678	T14677	arg1Of	Biotec,Miltenyi
R11452	T14679	T14676	arg3Of	),(
R11453	T14681	T14680	arg1Of	mice,All
R11454	T14681	T14682	arg1Of	mice,were
R11455	T14681	T14683	arg2Of	mice,maintained
R11456	T14681	T14690	arg2Of	mice,used
R11457	T14683	T14684	arg1Of	maintained,in
R11458	T14683	T14689	arg1Of	maintained,and
R11459	T14688	T14684	arg2Of	facilities,in
R11460	T14688	T14685	arg1Of	facilities,specific
R11461	T14688	T14686	arg1Of	facilities,pathogen-free
R11462	T14688	T14687	arg1Of	facilities,barrier
R11463	T14689	T14682	arg2Of	and,were
R11464	T14689	T14712	arg1Of	and,","
R11465	T14690	T14689	arg2Of	used,and
R11466	T14690	T14691	arg1Of	used,according
R11467	T14690	T14710	arg1Of	used,For
R11468	T14692	T14691	arg2Of	to,according
R11469	T14693	T14694	arg2Of	protocols,approved
R11470	T14693	T14700	arg1Of	protocols,and
R11471	T14699	T14694	arg1Of	Institute,approved
R11472	T14699	T14695	arg2Of	Institute,by
R11473	T14699	T14696	arg1Of	Institute,the
R11474	T14699	T14697	arg1Of	Institute,Immune
R11475	T14699	T14698	arg1Of	Institute,Disease
R11476	T14700	T14692	arg2Of	and,to
R11477	T14706	T14702	arg1Of	Care,Harvard
R11478	T14706	T14703	arg1Of	Care,Medical
R11479	T14706	T14704	arg1Of	Care,School
R11480	T14706	T14705	arg1Of	Care,Animal
R11481	T14706	T14707	arg1Of	Care,and
R11482	T14708	T14707	arg2Of	Use,and
R11483	T14709	T14700	arg2Of	Committees.,and
R11484	T14709	T14701	arg1Of	Committees.,the
R11485	T14709	T14706	arg1Of	Committees.,Care
R11486	T14709	T14708	arg1Of	Committees.,Use
R11487	T14711	T14710	arg2Of	stimulation,For
R11488	T14716	T14713	arg2Of	cells,purified
R11489	T14716	T14714	arg1Of	cells,CD8+
R11490	T14716	T14715	arg1Of	cells,T
R11491	T14716	T14717	arg1Of	cells,were
R11492	T14716	T14718	arg2Of	cells,cultured
R11493	T14718	T14712	arg2Of	cultured,","
R11494	T14718	T14717	arg2Of	cultured,were
R11495	T14718	T14719	arg1Of	cultured,at
R11496	T14721	T14719	arg2Of	cells/ml,at
R11497	T14721	T14720	arg1Of	cells/ml,106
R11498	T14721	T14722	arg1Of	cells/ml,(
R11499	T14721	T14726	arg1Of	cells/ml,in
R11500	T14724	T14722	arg2Of	ml,(
R11501	T14724	T14723	arg1Of	ml,10
R11502	T14725	T14722	arg3Of	),(
R11503	T14728	T14726	arg2Of	flasks,in
R11504	T14728	T14727	arg1Of	flasks,T25
R11505	T14728	T14729	arg2Of	flasks,coated
R11506	T14729	T14730	arg1Of	coated,with
R11507	T14732	T14730	arg2Of	μg/ml,with
R11508	T14732	T14731	arg1Of	μg/ml,1
R11509	T14732	T14733	arg1Of	μg/ml,each
R11510	T14732	T14734	arg1Of	μg/ml,of
R11511	T14735	T14736	arg1Of	anti-CD3,(
R11512	T14735	T14740	arg1Of	anti-CD3,and
R11513	T14738	T14736	arg2Of	2C11,(
R11514	T14738	T14737	arg1Of	2C11,clone
R11515	T14739	T14736	arg3Of	),(
R11516	T14740	T14734	arg2Of	and,of
R11517	T14740	T14746	arg1Of	and,by
R11518	T14743	T14740	arg2Of	clone,and
R11519	T14743	T14741	arg1Of	clone,anti-CD28
R11520	T14743	T14742	arg2Of	clone,(
R11521	T14743	T14744	arg1Of	clone,37.51
R11522	T14745	T14742	arg3Of	),(
R11523	T14747	T14746	arg2Of	pretreatment,by
R11524	T14747	T14748	arg1Of	pretreatment,with
R11525	T14753	T14748	arg2Of	IgG,with
R11526	T14753	T14749	arg1Of	IgG,300
R11527	T14753	T14750	arg1Of	IgG,μg/ml
R11528	T14753	T14751	arg1Of	IgG,goat
R11529	T14753	T14752	arg1Of	IgG,anti–hamster
R11530	T14756	T14754	arg2Of	h,After
R11531	T14756	T14755	arg1Of	h,48
R11532	T14758	T14759	arg1Of	cells,were
R11533	T14758	T14760	arg2Of	cells,removed
R11534	T14758	T14766	arg2Of	cells,recultured
R11535	T14760	T14761	arg1Of	removed,from
R11536	T14760	T14765	arg1Of	removed,and
R11537	T14764	T14761	arg2Of	stimulation,from
R11538	T14764	T14762	arg1Of	stimulation,the
R11539	T14764	T14763	arg1Of	stimulation,TCR
R11540	T14765	T14754	arg1Of	and,After
R11541	T14765	T14757	arg1Of	and,","
R11542	T14765	T14759	arg2Of	and,were
R11543	T14766	T14765	arg2Of	recultured,and
R11544	T14766	T14767	arg1Of	recultured,at
R11545	T14769	T14767	arg2Of	concentration,at
R11546	T14769	T14768	arg1Of	concentration,a
R11547	T14769	T14770	arg1Of	concentration,of
R11548	T14769	T14775	arg1Of	concentration,in
R11549	T14773	T14772	arg1Of	105,×
R11550	T14774	T14770	arg2Of	cells/ml,of
R11551	T14774	T14771	arg1Of	cells/ml,5
R11552	T14774	T14773	arg1Of	cells/ml,105
R11553	T14776	T14775	arg2Of	media,in
R11554	T14776	T14777	arg2Of	media,supplemented
R11555	T14777	T14778	arg1Of	supplemented,with
R11556	T14781	T14778	arg2Of	rhIL-2,with
R11557	T14781	T14779	arg1Of	rhIL-2,100
R11558	T14781	T14780	arg1Of	rhIL-2,U/ml
R11559	T14784	T14782	arg1Of	h,Every
R11560	T14784	T14783	arg1Of	h,24
R11561	T14787	T14786	arg1Of	cells,viable
R11562	T14787	T14788	arg1Of	cells,were
R11563	T14787	T14789	arg2Of	cells,counted
R11564	T14787	T14791	arg2Of	cells,readjusted
R11565	T14789	T14790	arg1Of	counted,and
R11566	T14790	T14784	arg1Of	and,h
R11567	T14790	T14785	arg1Of	and,","
R11568	T14790	T14788	arg2Of	and,were
R11569	T14791	T14790	arg2Of	readjusted,and
R11570	T14791	T14792	arg1Of	readjusted,to
R11571	T14795	T14794	arg1Of	105,×
R11572	T14796	T14792	arg2Of	cells/ml,to
R11573	T14796	T14793	arg1Of	cells/ml,5
R11574	T14796	T14795	arg1Of	cells/ml,105
R11575	T14796	T14797	arg1Of	cells/ml,with
R11576	T14799	T14797	arg2Of	media,with
R11577	T14799	T14798	arg1Of	media,fresh
R11578	T14799	T14800	arg1Of	media,containing
R11579	T14803	T14800	arg2Of	amount,containing
R11580	T14803	T14801	arg1Of	amount,the
R11581	T14803	T14802	arg1Of	amount,corresponding
R11582	T14803	T14804	arg1Of	amount,of
R11583	T14805	T14804	arg2Of	rhIL-2,of
R11973	T15411	T15412	arg1Of	Isolation,of
R11974	T15415	T15412	arg2Of	cells,of
R11975	T15415	T15413	arg1Of	cells,CD8+
R11976	T15415	T15414	arg1Of	cells,T
R11977	T15415	T15416	arg1Of	cells,from
R11978	T15418	T15416	arg2Of	mice,from
R11979	T15418	T15417	arg1Of	mice,Runx3−/−
R11980	T15421	T15419	arg1Of	cells,Runx3-deficient
R11981	T15421	T15420	arg1Of	cells,T
R11982	T15421	T15422	arg1Of	cells,fail
R11983	T15422	T15423	arg1Of	fail,to
R11984	T15422	T15432	arg1Of	fail,(
R11985	T15426	T15423	arg2Of	expression,to
R11986	T15426	T15424	arg1Of	expression,silence
R11987	T15426	T15425	arg1Of	expression,CD4
R11988	T15426	T15427	arg1Of	expression,normally
R11989	T15426	T15428	arg1Of	expression,(
R11990	T15430	T15428	arg2Of	S1,(
R11991	T15430	T15429	arg1Of	S1,Fig.
R11992	T15431	T15428	arg3Of	),(
R11993	T15433	T15432	arg2Of	12,(
R11994	T15433	T15434	arg1Of	12,","
R11995	T15435	T15434	arg2Of	13,","
R11996	T15436	T15432	arg3Of	),(
R11997	T15437	T15440	arg1Of	We,fractionated
R11998	T15440	T15438	arg1Of	fractionated,therefore
R11999	T15440	T15439	arg1Of	fractionated,further
R12000	T15440	T15451	arg1Of	fractionated,into
R12001	T15440	T15460	modOf	fractionated,using
R12002	T15443	T15442	arg1Of	selected,positively
R12003	T15446	T15440	arg2Of	cells,fractionated
R12004	T15446	T15441	arg1Of	cells,the
R12005	T15446	T15443	arg1Of	cells,selected
R12006	T15446	T15444	arg1Of	cells,CD8+
R12007	T15446	T15445	arg1Of	cells,T
R12008	T15446	T15447	arg1Of	cells,from
R12009	T15450	T15447	arg2Of	mice,from
R12010	T15450	T15448	arg1Of	mice,Runx3
R12011	T15450	T15449	arg1Of	mice,KO
R12012	T15453	T15452	arg1Of	SP,CD8+CD4−
R12013	T15453	T15454	arg1Of	SP,or
R12014	T15454	T15451	arg2Of	or,into
R12015	T15454	T15458	arg1Of	or,by
R12016	T15457	T15454	arg2Of	cells,or
R12017	T15457	T15455	arg1Of	cells,CD8+CD4+
R12018	T15457	T15456	arg1Of	cells,DP
R12019	T15459	T15458	arg2Of	separation,by
R12020	T15462	T15461	arg1Of	magnetic,anti-CD4
R12021	T15463	T15460	arg2Of	beads,using
R12022	T15463	T15462	arg1Of	beads,magnetic
R12023	T15464	T15465	arg1Of	This,yielded
R12024	T15465	T15478	arg1Of	yielded,and
R12025	T15469	T15466	arg1Of	SP,a
R12026	T15469	T15467	arg1Of	SP,Runx3
R12027	T15469	T15468	arg1Of	SP,KO
R12028	T15469	T15470	arg1Of	SP,“enriched”
R12029	T15471	T15470	arg2Of	population,“enriched”
R12030	T15472	T15465	arg2Of	that,yielded
R12031	T15472	T15473	arg1Of	that,contained
R12032	T15473	T15470	arg3Of	contained,“enriched”
R12033	T15474	T15475	arg1Of	75,%
R12034	T15477	T15473	arg2Of	cells,contained
R12035	T15477	T15474	arg1Of	cells,75
R12036	T15477	T15476	arg1Of	cells,CD8+CD4−
R12037	T15481	T15479	arg1Of	DP,a
R12038	T15481	T15480	arg1Of	DP,KO
R12039	T15481	T15482	arg1Of	DP,enriched
R12040	T15482	T15478	arg2Of	enriched,and
R12041	T15483	T15482	arg2Of	population,enriched
R12042	T15483	T15484	arg1Of	population,that
R12043	T15483	T15485	arg1Of	population,contained
R12044	T15486	T15487	arg1Of	85,%
R12045	T15489	T15485	arg2Of	cells,contained
R12046	T15489	T15486	arg1Of	cells,85
R12047	T15489	T15488	arg1Of	cells,CD8+CD4+
R12048	T15489	T15490	arg1Of	cells,(
R12049	T15492	T15490	arg2Of	S1,(
R12050	T15492	T15491	arg1Of	S1,Fig.
R12051	T15493	T15490	arg3Of	),(
R12052	T15495	T15494	arg1Of	cells,The
R12053	T15495	T15496	arg1Of	cells,were
R12054	T15495	T15497	arg2Of	cells,stimulated
R12055	T15495	T15505	arg1Of	cells,removing
R12056	T15495	T15512	arg1Of	cells,culturing
R12057	T15497	T15496	arg2Of	stimulated,were
R12058	T15497	T15498	arg1Of	stimulated,with
R12059	T15497	T15501	arg1Of	stimulated,for
R12060	T15497	T15504	arg1Of	stimulated,before
R12061	T15500	T15498	arg2Of	anti-CD28,with
R12062	T15500	T15499	arg1Of	anti-CD28,anti-CD3+
R12063	T15503	T15501	arg2Of	d,for
R12064	T15503	T15502	arg1Of	d,2
R12065	T15505	T15507	arg1Of	removing,from
R12066	T15505	T15511	arg1Of	removing,and
R12067	T15506	T15505	arg2Of	them,removing
R12068	T15510	T15507	arg2Of	stimulus,from
R12069	T15510	T15508	arg1Of	stimulus,the
R12070	T15510	T15509	arg1Of	stimulus,TCR
R12071	T15511	T15504	arg2Of	and,before
R12072	T15512	T15511	arg2Of	culturing,and
R12073	T15512	T15514	arg1Of	culturing,in
R12074	T15513	T15512	arg2Of	them,culturing
R12075	T15515	T15514	arg2Of	media,in
R12076	T15515	T15516	arg1Of	media,containing
R12077	T15519	T15516	arg2Of	IL-2,containing
R12078	T15519	T15517	arg1Of	IL-2,100
R12079	T15519	T15518	arg1Of	IL-2,U/ml
R12080	T15522	T15520	arg2Of	reported,As
R12081	T15522	T15521	arg1Of	reported,previously
R12082	T15525	T15524	arg1Of	proliferation,TCR-induced
R12083	T15525	T15526	arg1Of	proliferation,of
R12084	T15525	T15531	arg1Of	proliferation,was
R12085	T15525	T15533	arg1Of	proliferation,impaired
R12086	T15525	T15535	arg1Of	proliferation,irrespective
R12087	T15530	T15526	arg2Of	cells,of
R12088	T15530	T15527	arg1Of	cells,Runx3−/−
R12089	T15530	T15528	arg1Of	cells,CD8+
R12090	T15530	T15529	arg1Of	cells,T
R12091	T15531	T15520	arg1Of	was,As
R12092	T15531	T15523	arg1Of	was,","
R12093	T15531	T15543	arg1Of	was,(
R12094	T15533	T15534	arg1Of	impaired,","
R12095	T15534	T15531	arg2Of	",",was
R12096	T15534	T15532	arg1Of	",",severely
R12097	T15535	T15534	arg2Of	irrespective,","
R12098	T15535	T15536	arg1Of	irrespective,of
R12099	T15538	T15536	arg2Of	expression,of
R12100	T15538	T15537	arg1Of	expression,CD4
R12101	T15538	T15539	arg1Of	expression,(
R12102	T15541	T15539	arg2Of	S1,(
R12103	T15541	T15540	arg1Of	S1,Fig.
R12104	T15542	T15539	arg3Of	),(
R12105	T15544	T15543	arg2Of	12,(
R12106	T15544	T15545	arg1Of	12,","
R12107	T15546	T15545	arg2Of	13,","
R12108	T15547	T15543	arg3Of	),(
R12109	T15552	T15550	arg1Of	cells,the
R12110	T15552	T15551	arg1Of	cells,Runx3−/−
R12111	T15552	T15553	arg1Of	cells,showed
R12112	T15553	T15548	arg1Of	showed,However
R12113	T15553	T15549	arg1Of	showed,","
R12114	T15556	T15553	arg2Of	patterns,showed
R12115	T15556	T15554	arg1Of	patterns,cell-surface
R12116	T15556	T15555	arg1Of	patterns,expression
R12117	T15556	T15557	arg1Of	patterns,indicative
R12118	T15556	T15561	arg1Of	patterns,","
R12119	T15556	T15562	arg1Of	patterns,including
R12120	T15557	T15558	arg1Of	indicative,of
R12121	T15560	T15558	arg2Of	cells,of
R12122	T15560	T15559	arg2Of	cells,activated
R12123	T15563	T15564	arg1Of	up-regulation,of
R12124	T15563	T15566	arg1Of	up-regulation,and
R12125	T15565	T15564	arg2Of	CD25,of
R12126	T15566	T15562	arg2Of	and,including
R12127	T15567	T15566	arg2Of	CD69,and
R12128	T15567	T15568	arg1Of	CD69,(
R12129	T15570	T15568	arg2Of	S1,(
R12130	T15570	T15569	arg1Of	S1,Fig.
R12131	T15571	T15568	arg3Of	),(
R12132	T15573	T15572	arg2Of	expected,As
R12133	T15573	T15574	arg1Of	expected,from
R12134	T15576	T15574	arg2Of	ability,from
R12135	T15576	T15575	arg1Of	ability,their
R12136	T15576	T15577	modOf	ability,to
R12137	T15578	T15577	arg1Of	up-regulate,to
R12138	T15579	T15578	arg2Of	CD25,up-regulate
R12139	T15584	T15581	arg1Of	cells,Runx3−/−
R12140	T15584	T15582	arg1Of	cells,CD8+
R12141	T15584	T15583	arg1Of	cells,T
R12142	T15584	T15585	arg1Of	cells,responded
R12143	T15584	T15594	arg2Of	cells,expanded
R12144	T15585	T15586	arg1Of	responded,to
R12145	T15585	T15589	arg1Of	responded,after
R12146	T15585	T15592	arg1Of	responded,and
R12147	T15588	T15586	arg2Of	supplementation,to
R12148	T15588	T15587	arg1Of	supplementation,IL-2
R12149	T15590	T15589	arg2Of	day,after
R12150	T15590	T15591	arg1Of	day,2
R12151	T15592	T15572	arg1Of	and,As
R12152	T15592	T15580	arg1Of	and,","
R12153	T15594	T15592	arg2Of	expanded,and
R12154	T15594	T15593	arg1Of	expanded,efficiently
R12155	T15594	T15595	arg1Of	expanded,until
R12156	T15594	T15602	arg1Of	expanded,","
R12157	T15594	T15604	arg1Of	expanded,at
R12158	T15596	T15595	arg2Of	day,until
R12159	T15596	T15597	arg1Of	day,6
R12160	T15596	T15598	arg1Of	day,of
R12161	T15601	T15598	arg2Of	period,of
R12162	T15601	T15599	arg1Of	period,the
R12163	T15601	T15600	arg1Of	period,culture
R12164	T15604	T15603	arg1Of	at,albeit
R12165	T15606	T15604	arg2Of	rates,at
R12166	T15606	T15605	arg1Of	rates,slower
R12167	T15606	T15607	arg1Of	rates,compared
R12168	T15608	T15607	arg2Of	with,compared
R12169	T15610	T15608	arg2Of	cells,with
R12170	T15610	T15609	arg1Of	cells,WT
R12171	T15610	T15611	arg1Of	cells,(
R12172	T15613	T15611	arg2Of	S1,(
R12173	T15613	T15612	arg1Of	S1,Fig.
R12174	T15614	T15611	arg3Of	),(
R12175	T15617	T15616	arg1Of	fraction,a
R12176	T15617	T15618	arg1Of	fraction,of
R12177	T15617	T15623	arg1Of	fraction,silenced
R12178	T15622	T15618	arg2Of	cells,of
R12179	T15622	T15619	arg1Of	cells,the
R12180	T15622	T15620	arg1Of	cells,KO
R12181	T15622	T15621	arg1Of	cells,DP
R12182	T15623	T15615	arg2Of	silenced,Although
R12183	T15623	T15626	arg1Of	silenced,after
R12184	T15625	T15623	arg2Of	expression,silenced
R12185	T15625	T15624	arg1Of	expression,CD4
R12186	T15627	T15626	arg2Of	activation,after
R12187	T15630	T15629	arg1Of	ratio,the
R12188	T15630	T15631	arg1Of	ratio,of
R12189	T15630	T15634	arg1Of	ratio,in
R12190	T15630	T15638	arg1Of	ratio,remained
R12191	T15630	T15639	arg1Of	ratio,constant
R12192	T15633	T15631	arg2Of	cells,of
R12193	T15633	T15632	arg1Of	cells,SP/DP
R12194	T15637	T15634	arg2Of	population,in
R12195	T15637	T15635	arg1Of	population,each
R12196	T15637	T15636	arg2Of	population,enriched
R12197	T15638	T15615	arg1Of	remained,Although
R12198	T15638	T15628	arg1Of	remained,","
R12199	T15638	T15640	arg1Of	remained,thereafter
R12200	T15638	T15642	arg1Of	remained,and
R12201	T15639	T15638	arg2Of	constant,remained
R12202	T15642	T15641	arg1Of	and,","
R12203	T15643	T15644	arg1Of	we,did
R12204	T15643	T15646	arg1Of	we,observe
R12205	T15646	T15642	arg2Of	observe,and
R12206	T15646	T15644	arg2Of	observe,did
R12207	T15646	T15645	arg1Of	observe,not
R12208	T15646	T15658	arg1Of	observe,","
R12209	T15646	T15659	modOf	observe,indicating
R12210	T15649	T15646	arg2Of	differences,observe
R12211	T15649	T15647	arg1Of	differences,any
R12212	T15649	T15648	arg1Of	differences,major
R12213	T15649	T15650	arg1Of	differences,between
R12214	T15653	T15650	arg2Of	populations,between
R12215	T15653	T15651	arg1Of	populations,these
R12216	T15653	T15652	arg1Of	populations,two
R12217	T15653	T15654	arg1Of	populations,throughout
R12218	T15657	T15654	arg2Of	period,throughout
R12219	T15657	T15655	arg1Of	period,the
R12220	T15657	T15656	arg1Of	period,culture
R12221	T15661	T15667	arg1Of	in,and
R12222	T15662	T15661	arg2Of	terms,in
R12223	T15662	T15663	arg1Of	terms,of
R12224	T15666	T15663	arg2Of	differentiation,of
R12225	T15666	T15664	arg1Of	differentiation,effector
R12226	T15666	T15665	arg1Of	differentiation,CTL
R12227	T15668	T15667	arg2Of	under,and
R12228	T15671	T15668	arg2Of	conditions,under
R12229	T15671	T15669	arg1Of	conditions,our
R12230	T15671	T15670	arg1Of	conditions,culture
R12231	T15676	T15673	arg1Of	cells,Runx3−/−
R12232	T15676	T15674	arg1Of	cells,CD8+
R12233	T15676	T15675	arg1Of	cells,T
R12234	T15676	T15677	arg1Of	cells,that
R12235	T15676	T15679	arg1Of	cells,coexpress
R12236	T15676	T15681	arg1Of	cells,are
R12237	T15676	T15682	arg1Of	cells,indistinguishable
R12238	T15679	T15678	arg1Of	coexpress,also
R12239	T15680	T15679	arg2Of	CD4,coexpress
R12240	T15681	T15659	arg2Of	are,indicating
R12241	T15681	T15660	arg1Of	are,that
R12242	T15681	T15661	arg1Of	are,in
R12243	T15681	T15668	arg1Of	are,under
R12244	T15681	T15672	arg1Of	are,","
R12245	T15682	T15681	arg2Of	indistinguishable,are
R12246	T15682	T15683	arg1Of	indistinguishable,from
R12247	T15684	T15683	arg2Of	those,from
R12248	T15684	T15685	arg1Of	those,that
R12249	T15684	T15686	arg1Of	those,do
R12250	T15686	T15687	arg1Of	do,not
R12251	T15689	T15688	arg1Of	data,The
R12252	T15689	T15690	arg2Of	data,presented
R12253	T15689	T15694	arg1Of	data,are
R12254	T15689	T15709	arg1Of	data,are
R12255	T15689	T15710	arg1Of	data,from
R12256	T15690	T15691	arg1Of	presented,in
R12257	T15693	T15691	arg2Of	S2,in
R12258	T15693	T15692	arg1Of	S2,Fig.
R12259	T15694	T15695	arg1Of	are,from
R12260	T15694	T15700	arg1Of	are,","
R12261	T15694	T15701	arg1Of	are,whereas
R12262	T15699	T15695	arg2Of	cells,from
R12263	T15699	T15696	arg1Of	cells,Runx3
R12264	T15699	T15697	arg1Of	cells,KO
R12265	T15699	T15698	arg1Of	cells,SP
R12266	T15702	T15703	arg2Of	those,shown
R12267	T15703	T15704	arg1Of	shown,in
R12268	T15705	T15706	arg1Of	Figs.,3
R12269	T15705	T15707	arg1Of	Figs.,and
R12270	T15707	T15704	arg2Of	and,in
R12271	T15708	T15707	arg2Of	4,and
R12272	T15709	T15701	arg2Of	are,whereas
R12273	T15709	T15703	modOf	are,shown
R12274	T15710	T15694	arg2Of	from,are
R12275	T15710	T15709	arg2Of	from,are
R12276	T15715	T15710	arg2Of	cells,from
R12277	T15715	T15711	arg1Of	cells,total
R12278	T15715	T15712	arg1Of	cells,Runx3
R12279	T15715	T15713	arg1Of	cells,KO
R12280	T15715	T15714	arg1Of	cells,CD8
R12451	T16231	T16229	arg1Of	assay,FACS-based
R12452	T16231	T16230	arg1Of	assay,cytotoxicity
R12453	T16233	T16232	arg1Of	measure,To
R12454	T16234	T16233	arg2Of	cytotoxicity,measure
R12455	T16239	T16233	arg1Of	cells,measure
R12456	T16239	T16236	arg1Of	cells,EL4
R12457	T16239	T16237	arg1Of	cells,thymoma
R12458	T16239	T16238	arg1Of	cells,target
R12459	T16239	T16240	arg1Of	cells,were
R12460	T16239	T16241	arg2Of	cells,loaded
R12461	T16241	T16232	modOf	loaded,To
R12462	T16241	T16235	arg1Of	loaded,","
R12463	T16241	T16240	arg2Of	loaded,were
R12464	T16241	T16242	arg1Of	loaded,with
R12465	T16241	T16252	arg1Of	loaded,before
R12466	T16241	T16261	arg1Of	loaded,at
R12467	T16243	T16244	arg1Of	0,or
R12468	T16244	T16246	arg1Of	or,μM
R12469	T16245	T16244	arg2Of	1,or
R12470	T16248	T16242	arg2Of	peptide,with
R12471	T16248	T16243	arg1Of	peptide,0
R12472	T16248	T16245	arg1Of	peptide,1
R12473	T16248	T16247	arg1Of	peptide,Gp33
R12474	T16248	T16249	arg1Of	peptide,for
R12475	T16251	T16249	arg2Of	h,for
R12476	T16251	T16250	arg1Of	h,2
R12477	T16255	T16252	arg2Of	coincubation,before
R12478	T16255	T16253	arg1Of	coincubation,a
R12479	T16255	T16254	arg1Of	coincubation,2-h
R12480	T16255	T16256	arg1Of	coincubation,with
R12481	T16260	T16256	arg2Of	cells,with
R12482	T16260	T16257	arg1Of	cells,P14
R12483	T16260	T16258	arg1Of	cells,CD8+
R12484	T16260	T16259	arg1Of	cells,T
R12485	T16264	T16261	arg2Of	ratios,at
R12486	T16264	T16262	arg1Of	ratios,the
R12487	T16264	T16263	arg1Of	ratios,effector-to-target
R12488	T16264	T16265	arg2Of	ratios,indicated
R12489	T16265	T16266	arg1Of	indicated,in
R12490	T16268	T16266	arg2Of	figures,in
R12491	T16268	T16267	arg1Of	figures,the
R12492	T16268	T16269	arg1Of	figures,in
R12493	T16272	T16269	arg2Of	plates,in
R12494	T16272	T16270	arg1Of	plates,96-well
R12495	T16272	T16271	arg1Of	plates,round-bottom
R12496	T16276	T16273	arg2Of	period,After
R12497	T16276	T16274	arg1Of	period,the
R12498	T16276	T16275	arg1Of	period,coincubation
R12499	T16278	T16279	arg1Of	cells,were
R12500	T16278	T16280	arg2Of	cells,stained
R12501	T16280	T16273	arg1Of	stained,After
R12502	T16280	T16277	arg1Of	stained,","
R12503	T16280	T16279	arg2Of	stained,were
R12504	T16280	T16281	arg1Of	stained,with
R12505	T16283	T16284	arg1Of	V–FITC,and
R12506	T16284	T16281	arg2Of	and,with
R12507	T16284	T16282	arg1Of	and,Annexin
R12508	T16285	T16284	arg2Of	anti-CD8–allophycocyanin,and
R12509	T16287	T16286	arg1Of	analysis,Data
R12510	T16287	T16288	arg1Of	analysis,was
R12511	T16287	T16289	arg2Of	analysis,performed
R12512	T16289	T16288	arg2Of	performed,was
R12513	T16289	T16290	arg1Of	performed,with
R12514	T16289	T16293	arg1Of	performed,(
R12515	T16289	T16299	arg1Of	performed,;
R12516	T16292	T16290	arg2Of	software,with
R12517	T16292	T16291	arg1Of	software,FlowJo
R12518	T16295	T16293	arg2Of	Star,(
R12519	T16295	T16294	arg1Of	Star,Tree
R12520	T16295	T16296	arg1Of	Star,","
R12521	T16297	T16296	arg2Of	Inc.,","
R12522	T16298	T16293	arg3Of	),(
R12523	T16302	T16300	arg1Of	cells,EL4
R12524	T16302	T16301	arg1Of	cells,target
R12525	T16302	T16303	arg1Of	cells,(
R12526	T16302	T16307	arg1Of	cells,were
R12527	T16302	T16308	arg2Of	cells,gated
R12528	T16305	T16303	arg2Of	events,(
R12529	T16305	T16304	arg1Of	events,CD8-negative
R12530	T16306	T16303	arg3Of	),(
R12531	T16308	T16307	arg2Of	gated,were
R12532	T16308	T16310	arg1Of	gated,and
R12533	T16310	T16299	arg2Of	and,;
R12534	T16310	T16309	arg1Of	and,","
R12535	T16312	T16311	arg1Of	percentage,the
R12536	T16312	T16313	arg1Of	percentage,of
R12537	T16312	T16318	arg1Of	percentage,was
R12538	T16312	T16319	arg2Of	percentage,determined
R12539	T16317	T16313	arg2Of	cells,of
R12540	T16317	T16314	arg1Of	cells,Annexin
R12541	T16317	T16315	arg1Of	cells,V+
R12542	T16317	T16316	arg1Of	cells,target
R12543	T16319	T16310	arg2Of	determined,and
R12544	T16319	T16318	arg2Of	determined,was
R12670	T16510	T16511	arg1Of	Cytokine,and
R12671	T16512	T16511	arg2Of	surface,and
R12672	T16514	T16510	arg1Of	staining,Cytokine
R12673	T16514	T16512	arg1Of	staining,surface
R12674	T16514	T16513	arg1Of	staining,marker
R12679	T16520	T16521	arg1Of	cells,were
R12680	T16520	T16522	arg2Of	cells,restimulated
R12681	T16520	T16536	arg2Of	cells,indicated
R12682	T16522	T16521	arg2Of	restimulated,were
R12683	T16522	T16523	arg1Of	restimulated,with
R12684	T16522	T16535	arg1Of	restimulated,unless
R12685	T16522	T16543	arg1Of	restimulated,and
R12686	T16524	T16525	arg1Of	10,nM
R12687	T16528	T16527	arg1Of	1,+
R12688	T16530	T16523	arg2Of	ionomycin,with
R12689	T16530	T16524	arg1Of	ionomycin,10
R12690	T16530	T16526	arg1Of	ionomycin,PMA
R12691	T16530	T16528	arg1Of	ionomycin,1
R12692	T16530	T16529	arg1Of	ionomycin,μM
R12693	T16530	T16531	arg1Of	ionomycin,for
R12694	T16533	T16531	arg2Of	h,for
R12695	T16533	T16532	arg1Of	h,6
R12696	T16533	T16534	arg1Of	h,(
R12697	T16536	T16535	arg2Of	indicated,unless
R12698	T16536	T16537	arg1Of	indicated,otherwise
R12699	T16536	T16538	arg1Of	indicated,in
R12700	T16540	T16538	arg2Of	figures,in
R12701	T16540	T16539	arg1Of	figures,the
R12702	T16540	T16541	arg1Of	figures,)
R12703	T16543	T16515	modOf	and,To
R12704	T16543	T16519	arg1Of	and,","
R12705	T16543	T16542	arg1Of	and,","
R12706	T16546	T16544	arg1Of	stains,intracellular
R12707	T16546	T16545	arg1Of	stains,cytokine
R12708	T16546	T16547	arg1Of	stains,were
R12709	T16546	T16548	arg2Of	stains,performed
R12710	T16548	T16543	arg2Of	performed,and
R12711	T16548	T16547	arg2Of	performed,were
R12712	T16548	T16549	arg1Of	performed,as
R12713	T16551	T16549	arg2Of	described,as
R12714	T16551	T16550	arg1Of	described,previously
R12715	T16551	T16552	arg1Of	described,(
R12716	T16553	T16552	arg2Of	28,(
R12717	T16554	T16552	arg3Of	),(
R12718	T16556	T16555	arg1Of	detect,To
R12719	T16557	T16556	arg2Of	expression,detect
R12720	T16557	T16558	arg1Of	expression,of
R12721	T16560	T16558	arg2Of	molecules,of
R12722	T16560	T16559	arg1Of	molecules,surface
R12723	T16562	T16556	arg1Of	cells,detect
R12724	T16562	T16563	arg1Of	cells,were
R12725	T16562	T16564	arg2Of	cells,washed
R12726	T16562	T16568	arg1Of	cells,resuspended
R12727	T16562	T16602	arg2Of	cells,incubated
R12728	T16562	T16613	arg1Of	cells,washed
R12729	T16562	T16616	arg1Of	cells,resuspended
R12730	T16564	T16563	arg2Of	washed,were
R12731	T16564	T16565	arg1Of	washed,in
R12732	T16564	T16567	arg1Of	washed,","
R12733	T16566	T16565	arg2Of	PBS,in
R12734	T16567	T16602	modOf	",",incubated
R12735	T16567	T16612	arg1Of	",",","
R12736	T16568	T16567	arg2Of	resuspended,","
R12737	T16568	T16569	arg1Of	resuspended,in
R12738	T16572	T16570	arg1Of	buffer,FACS
R12739	T16572	T16571	arg1Of	buffer,wash
R12740	T16572	T16573	arg1Of	buffer,(
R12741	T16572	T16590	arg1Of	buffer,containing
R12742	T16572	T16593	arg1Of	buffer,indicated
R12743	T16574	T16575	arg1Of	3,%
R12744	T16576	T16573	arg2Of	FBS,(
R12745	T16576	T16574	arg1Of	FBS,3
R12746	T16576	T16577	arg1Of	FBS,","
R12747	T16576	T16582	arg1Of	FBS,","
R12748	T16576	T16586	arg1Of	FBS,","
R12749	T16578	T16579	arg1Of	0.1,%
R12750	T16581	T16577	arg2Of	azide,","
R12751	T16581	T16578	arg1Of	azide,0.1
R12752	T16581	T16580	arg1Of	azide,sodium
R12753	T16584	T16583	arg1Of	mM,30
R12754	T16585	T16582	arg2Of	Hepes,","
R12755	T16585	T16584	arg1Of	Hepes,mM
R12756	T16588	T16586	arg2Of	PBS,","
R12757	T16588	T16587	arg1Of	PBS,1×
R12758	T16589	T16573	arg3Of	),(
R12759	T16592	T16590	arg2Of	antibodies,containing
R12760	T16592	T16591	arg1Of	antibodies,the
R12761	T16593	T16568	arg2Of	indicated,resuspended
R12762	T16593	T16594	arg1Of	indicated,in
R12763	T16593	T16597	arg1Of	indicated,at
R12764	T16593	T16601	arg1Of	indicated,","
R12765	T16596	T16594	arg2Of	figures,in
R12766	T16596	T16595	arg1Of	figures,the
R12767	T16599	T16598	arg1Of	optimized,previously
R12768	T16600	T16597	arg2Of	concentrations,at
R12769	T16600	T16599	arg1Of	concentrations,optimized
R12770	T16602	T16603	arg1Of	incubated,for
R12771	T16605	T16603	arg2Of	min,for
R12772	T16605	T16604	arg1Of	min,15
R12773	T16605	T16606	arg1Of	min,at
R12774	T16608	T16606	arg2Of	temperature,at
R12775	T16608	T16607	arg1Of	temperature,room
R12776	T16608	T16609	arg1Of	temperature,(
R12777	T16610	T16609	arg2Of	RT,(
R12778	T16611	T16609	arg3Of	),(
R12779	T16612	T16615	arg1Of	",",and
R12780	T16613	T16612	arg2Of	washed,","
R12781	T16615	T16555	modOf	and,To
R12782	T16615	T16561	arg1Of	and,","
R12783	T16615	T16614	arg1Of	and,","
R12784	T16616	T16615	arg2Of	resuspended,and
R12785	T16616	T16617	arg1Of	resuspended,in
R12786	T16616	T16623	arg1Of	resuspended,before
R12787	T16616	T16625	arg1Of	resuspended,on
R12788	T16618	T16619	arg1Of	2,%
R12789	T16622	T16617	arg2Of	solution,in
R12790	T16622	T16618	arg1Of	solution,2
R12791	T16622	T16620	arg1Of	solution,formaldehyde
R12792	T16622	T16621	arg1Of	solution,fixative
R12793	T16624	T16623	arg2Of	acquisition,before
R12794	T16627	T16625	arg2Of	FACSCalibur,on
R12795	T16627	T16626	arg1Of	FACSCalibur,a
R12796	T16627	T16628	arg1Of	FACSCalibur,(
R12797	T16629	T16628	arg2Of	BD,(
R12798	T16630	T16628	arg3Of	),(
R12928	T16786	T16785	arg1Of	transduction,Retroviral
R12929	T16786	T16787	arg1Of	transduction,of
R12930	T16791	T16787	arg2Of	cells,of
R12931	T16791	T16788	arg1Of	cells,primary
R12932	T16791	T16789	arg1Of	cells,CD8+
R12933	T16791	T16790	arg1Of	cells,T
R12935	T16794	T16792	arg2Of	experiments,For
R12936	T16797	T16796	arg1Of	supernatants,viral
R12937	T16797	T16798	arg1Of	supernatants,were
R12938	T16797	T16799	arg2Of	supernatants,generated
R12940	T16799	T16792	arg1Of	generated,For
R12941	T16799	T16798	arg2Of	generated,were
R12942	T16799	T16800	arg1Of	generated,by
R12943	T16803	T16801	arg1Of	transfection,calcium
R12944	T16803	T16802	arg1Of	transfection,phosphate
R12945	T16803	T16804	arg1Of	transfection,of
R12946	T16803	T16807	arg1Of	transfection,and
R12947	T16806	T16804	arg2Of	cells,of
R12948	T16806	T16805	arg1Of	cells,Phoenix
R12949	T16807	T16800	arg2Of	and,by
R12950	T16808	T16807	arg2Of	concentration,and
R12951	T16808	T16809	arg1Of	concentration,by
R12952	T16808	T16812	arg1Of	concentration,at
R12953	T16811	T16809	arg2Of	centrifugation,by
R12954	T16811	T16810	arg1Of	centrifugation,overnight
R12955	T16814	T16812	arg2Of	g,at
R12956	T16814	T16813	arg1Of	g,"6,000"
R12957	T16817	T16815	arg2Of	h,At
R12958	T16817	T16816	arg1Of	h,∼42
R12959	T16817	T16818	arg1Of	h,after
R12960	T16822	T16818	arg2Of	activation,after
R12961	T16822	T16819	arg1Of	activation,the
R12962	T16822	T16820	arg1Of	activation,initial
R12963	T16822	T16821	arg1Of	activation,TCR
R12964	T16822	T16823	arg1Of	activation,of
R12965	T16822	T16828	arg1Of	activation,per
R12966	T16827	T16823	arg2Of	cells,of
R12967	T16827	T16824	arg1Of	cells,106
R12968	T16827	T16825	arg1Of	cells,CD8+
R12969	T16827	T16826	arg1Of	cells,T
R12970	T16829	T16828	arg2Of	well,per
R12971	T16829	T16830	arg1Of	well,in
R12972	T16832	T16830	arg2Of	plates,in
R12973	T16832	T16831	arg1Of	plates,12-well
R12974	T16836	T16834	arg1Of	media,the
R12975	T16836	T16835	arg1Of	media,culture
R12976	T16836	T16837	arg1Of	media,was
R12977	T16836	T16838	arg2Of	media,removed
R12978	T16836	T16840	arg2Of	media,replaced
R12979	T16838	T16839	arg1Of	removed,and
R12980	T16839	T16815	arg1Of	and,At
R12981	T16839	T16833	arg1Of	and,","
R12982	T16839	T16837	arg2Of	and,was
R12983	T16840	T16839	arg2Of	replaced,and
R12984	T16840	T16841	arg1Of	replaced,with
R12985	T16843	T16841	arg2Of	media,with
R12986	T16843	T16842	arg1Of	media,complete
R12987	T16843	T16844	arg2Of	media,supplemented
R12988	T16844	T16845	arg1Of	supplemented,with
R12989	T16848	T16845	arg2Of	polybrene,with
R12990	T16848	T16846	arg1Of	polybrene,8
R12991	T16848	T16847	arg1Of	polybrene,μg/ml
R12992	T16848	T16849	arg1Of	polybrene,containing
R12993	T16850	T16851	arg1Of	fresh,plus
R12994	T16852	T16851	arg2Of	concentrated,plus
R12995	T16853	T16849	arg2Of	virus,containing
R12996	T16853	T16850	arg1Of	virus,fresh
R12997	T16853	T16852	arg1Of	virus,concentrated
R12998	T16855	T16854	arg1Of	plates,The
R12999	T16855	T16856	arg1Of	plates,were
R13000	T16855	T16857	arg2Of	plates,centrifuged
R13001	T16855	T16867	arg1Of	plates,returning
R13002	T16857	T16856	arg2Of	centrifuged,were
R13003	T16857	T16858	arg1Of	centrifuged,at
R13004	T16857	T16861	arg1Of	centrifuged,for
R13005	T16857	T16864	arg1Of	centrifuged,at
R13006	T16857	T16866	arg1Of	centrifuged,before
R13007	T16860	T16858	arg2Of	g,at
R13008	T16860	T16859	arg1Of	g,700
R13009	T16863	T16861	arg2Of	h,for
R13010	T16863	T16862	arg1Of	h,1
R13011	T16865	T16864	arg2Of	RT,at
R13012	T16867	T16866	arg2Of	returning,before
R13013	T16867	T16868	arg1Of	returning,to
R13014	T16867	T16870	arg1Of	returning,for
R13015	T16869	T16868	arg2Of	37°C,to
R13016	T16874	T16870	arg2Of	h,for
R13017	T16874	T16871	arg1Of	h,an
R13018	T16874	T16872	arg1Of	h,additional
R13019	T16874	T16873	arg1Of	h,5
R13020	T16876	T16875	arg1Of	constructs,Retroviral
R13021	T16876	T16877	arg1Of	constructs,for
R13022	T16876	T16885	arg1Of	constructs,were
R13023	T16878	T16879	arg1Of	Eomes-VP16,and
R13024	T16879	T16877	arg2Of	and,for
R13025	T16884	T16879	arg2Of	vector,and
R13026	T16884	T16880	arg1Of	vector,the
R13027	T16884	T16881	arg1Of	vector,MIG
R13028	T16884	T16882	arg1Of	vector,control
R13029	T16884	T16883	arg1Of	vector,empty
R13030	T16887	T16885	arg2Of	gift,were
R13031	T16887	T16886	arg1Of	gift,a
R13032	T16887	T16888	arg1Of	gift,from
R13033	T16890	T16888	arg2Of	Reiner,from
R13034	T16890	T16889	arg1Of	Reiner,S.L.
R13035	T16890	T16891	arg1Of	Reiner,(
R13036	T16890	T16900	arg1Of	Reiner,(
R13037	T16892	T16891	arg2Of	University,(
R13038	T16892	T16893	arg1Of	University,of
R13039	T16892	T16895	arg1Of	University,","
R13040	T16894	T16893	arg2Of	Pennsylvania,of
R13041	T16896	T16895	arg2Of	Philadelphia,","
R13042	T16896	T16897	arg1Of	Philadelphia,","
R13043	T16898	T16897	arg2Of	PA,","
R13044	T16899	T16891	arg3Of	),(
R13045	T16901	T16900	arg2Of	8,(
R13046	T16902	T16900	arg3Of	),(
R13047	T16967	T16963	arg1Of	analysis,ChIP
R13048	T16967	T16964	arg1Of	analysis,and
R13049	T16967	T16965	arg1Of	analysis,real-time
R13050	T16967	T16966	arg1Of	analysis,PCR
R13051	T16968	T16969	arg1Of	20,×
R13052	T16970	T16969	arg2Of	106,×
R13053	T16973	T16968	arg1Of	cells,20
R13054	T16973	T16970	arg1Of	cells,106
R13055	T16973	T16971	arg1Of	cells,CD8+
R13056	T16973	T16972	arg1Of	cells,T
R13057	T16973	T16974	arg1Of	cells,per
R13058	T16973	T16976	arg1Of	cells,were
R13059	T16973	T16977	arg2Of	cells,fixed
R13060	T16973	T17014	arg1Of	cells,were
R13061	T16973	T17015	arg2Of	cells,incubated
R13062	T16975	T16974	arg2Of	immunoprecipitation,per
R13063	T16977	T16976	arg2Of	fixed,were
R13064	T16977	T16978	arg1Of	fixed,by
R13065	T16977	T17013	arg1Of	fixed,and
R13066	T16979	T16978	arg2Of	adding,by
R13067	T16982	T16979	arg2Of	volume,adding
R13068	T16982	T16980	arg1Of	volume,a
R13069	T16982	T16981	arg1Of	volume,1/10th
R13070	T16982	T16983	arg1Of	volume,of
R13071	T16982	T16986	arg1Of	volume,(
R13072	T16982	T17007	arg1Of	volume,to
R13073	T16985	T16983	arg2Of	solution,of
R13074	T16985	T16984	arg1Of	solution,fixation
R13075	T16987	T16988	arg1Of	11.1,%
R13076	T16989	T16987	arg1Of	formaldehyde,11.1
R13077	T16989	T16990	arg1Of	formaldehyde,","
R13078	T16990	T16994	arg1Of	",",","
R13079	T16991	T16992	arg1Of	100,mM
R13080	T16993	T16990	arg2Of	NaCl,","
R13081	T16993	T16991	arg1Of	NaCl,100
R13082	T16994	T16998	arg1Of	",",","
R13083	T16995	T16996	arg1Of	1,mM
R13084	T16997	T16994	arg2Of	EDTA,","
R13085	T16997	T16995	arg1Of	EDTA,1
R13086	T16998	T17002	arg1Of	",",","
R13087	T16999	T17000	arg1Of	0.5,mM
R13088	T17001	T16998	arg2Of	EGTA,","
R13089	T17001	T16999	arg1Of	EGTA,0.5
R13090	T17002	T16986	arg2Of	",",(
R13091	T17003	T17004	arg1Of	50,mM
R13092	T17005	T17002	arg2Of	Hepes,","
R13093	T17005	T17003	arg1Of	Hepes,50
R13094	T17006	T16986	arg3Of	),(
R13095	T17009	T17007	arg2Of	volume,to
R13096	T17009	T17008	arg1Of	volume,1
R13097	T17009	T17010	arg1Of	volume,of
R13098	T17012	T17010	arg2Of	media,of
R13099	T17012	T17011	arg1Of	media,culture
R13100	T17015	T17013	arg2Of	incubated,and
R13101	T17015	T17014	arg2Of	incubated,were
R13102	T17015	T17016	arg1Of	incubated,for
R13103	T17017	T17018	arg1Of	10,or
R13104	T17019	T17018	arg2Of	30,or
R13105	T17020	T17016	arg2Of	min,for
R13106	T17020	T17017	arg1Of	min,10
R13107	T17020	T17019	arg1Of	min,30
R13108	T17020	T17021	arg1Of	min,at
R13109	T17022	T17021	arg2Of	RT,at
R13110	T17023	T17024	arg1Of	Fixation,was
R13111	T17023	T17025	arg2Of	Fixation,stopped
R13112	T17025	T17024	arg2Of	stopped,was
R13113	T17025	T17026	arg1Of	stopped,with
R13114	T17025	T17032	arg1Of	stopped,for
R13115	T17027	T17028	arg1Of	120,mM
R13116	T17029	T17026	arg2Of	glycine,with
R13117	T17029	T17027	arg1Of	glycine,120
R13118	T17029	T17030	arg1Of	glycine,on
R13119	T17031	T17030	arg2Of	ice,on
R13120	T17034	T17032	arg2Of	min,for
R13121	T17034	T17033	arg1Of	min,5
R13122	T17036	T17035	arg2Of	cells,Fixed
R13123	T17036	T17037	arg1Of	cells,were
R13124	T17036	T17038	arg2Of	cells,washed
R13125	T17038	T17037	arg2Of	washed,were
R13126	T17039	T17040	arg1Of	2×,with
R13127	T17039	T17072	arg1Of	2×,and
R13128	T17042	T17040	arg2Of	PBS,with
R13129	T17042	T17041	arg1Of	PBS,cold
R13130	T17042	T17043	arg1Of	PBS,","
R13131	T17044	T17043	arg2Of	1×,","
R13132	T17044	T17045	arg1Of	1×,with
R13133	T17047	T17045	arg2Of	solution,with
R13134	T17047	T17046	arg1Of	solution,cold
R13135	T17047	T17048	arg1Of	solution,I
R13136	T17047	T17049	arg1Of	solution,(
R13137	T17050	T17051	arg1Of	10,mM
R13138	T17052	T17050	arg1Of	Tris,10
R13139	T17052	T17053	arg1Of	Tris,[
R13140	T17052	T17057	arg1Of	Tris,","
R13141	T17054	T17053	arg2Of	pH,[
R13142	T17054	T17055	arg1Of	pH,7.5
R13143	T17056	T17053	arg3Of	],[
R13144	T17057	T17061	arg1Of	",",","
R13145	T17058	T17059	arg1Of	10,mM
R13146	T17060	T17057	arg2Of	EDTA,","
R13147	T17060	T17058	arg1Of	EDTA,10
R13148	T17061	T17065	arg1Of	",",","
R13149	T17062	T17063	arg1Of	0.5,mM
R13150	T17064	T17061	arg2Of	EGTA,","
R13151	T17064	T17062	arg1Of	EGTA,0.5
R13152	T17065	T17049	arg2Of	",",(
R13153	T17066	T17067	arg1Of	1,%
R13154	T17069	T17065	arg2Of	X-100,","
R13155	T17069	T17066	arg1Of	X-100,1
R13156	T17069	T17068	arg1Of	X-100,Triton
R13157	T17070	T17049	arg3Of	),(
R13158	T17072	T17038	arg3Of	and,washed
R13159	T17072	T17071	arg1Of	and,","
R13160	T17073	T17072	arg2Of	1×,and
R13161	T17073	T17074	arg1Of	1×,with
R13162	T17076	T17074	arg2Of	solution,with
R13163	T17076	T17075	arg1Of	solution,cold
R13164	T17076	T17077	arg1Of	solution,II
R13165	T17076	T17078	arg1Of	solution,(
R13166	T17079	T17080	arg1Of	10,mM
R13167	T17081	T17079	arg1Of	Tris,10
R13168	T17081	T17082	arg1Of	Tris,[
R13169	T17081	T17086	arg1Of	Tris,","
R13170	T17083	T17082	arg2Of	pH,[
R13171	T17083	T17084	arg1Of	pH,7.5
R13172	T17085	T17082	arg3Of	],[
R13173	T17086	T17090	arg1Of	",",","
R13174	T17087	T17088	arg1Of	1,mM
R13175	T17089	T17086	arg2Of	EDTA,","
R13176	T17089	T17087	arg1Of	EDTA,1
R13177	T17090	T17094	arg1Of	",",","
R13178	T17091	T17092	arg1Of	0.5,mM
R13179	T17093	T17090	arg2Of	EGTA,","
R13180	T17093	T17091	arg1Of	EGTA,0.5
R13181	T17094	T17078	arg2Of	",",(
R13182	T17095	T17096	arg1Of	200,mM
R13183	T17097	T17094	arg2Of	NaCl,","
R13184	T17097	T17095	arg1Of	NaCl,200
R13185	T17098	T17078	arg3Of	),(
R13186	T17100	T17099	arg2Of	washes,After
R13187	T17103	T17102	arg1Of	pellets,cell
R13188	T17103	T17104	arg1Of	pellets,were
R13189	T17103	T17105	arg2Of	pellets,resuspended
R13190	T17105	T17099	arg1Of	resuspended,After
R13191	T17105	T17101	arg1Of	resuspended,","
R13192	T17105	T17104	arg2Of	resuspended,were
R13193	T17105	T17106	arg1Of	resuspended,at
R13194	T17105	T17111	arg1Of	resuspended,in
R13195	T17105	T17147	arg1Of	resuspended,and
R13196	T17107	T17108	arg1Of	40,×
R13197	T17109	T17108	arg2Of	106,×
R13198	T17110	T17106	arg2Of	cells/ml,at
R13199	T17110	T17107	arg1Of	cells/ml,40
R13200	T17110	T17109	arg1Of	cells/ml,106
R13201	T17114	T17111	arg2Of	buffer,in
R13202	T17114	T17112	arg1Of	buffer,ChIP
R13203	T17114	T17113	arg1Of	buffer,lysis
R13204	T17114	T17115	arg1Of	buffer,(
R13205	T17118	T17116	arg1Of	NaCl,150
R13206	T17118	T17117	arg1Of	NaCl,mM
R13207	T17118	T17119	arg1Of	NaCl,","
R13208	T17119	T17127	arg1Of	",",","
R13209	T17120	T17121	arg1Of	25,mM
R13210	T17122	T17119	arg2Of	Tris,","
R13211	T17122	T17120	arg1Of	Tris,25
R13212	T17122	T17123	arg1Of	Tris,[
R13213	T17124	T17123	arg2Of	pH,[
R13214	T17124	T17125	arg1Of	pH,7.5
R13215	T17126	T17123	arg3Of	],[
R13216	T17127	T17132	arg1Of	",",","
R13217	T17128	T17129	arg1Of	1,%
R13218	T17131	T17127	arg2Of	X-100,","
R13219	T17131	T17128	arg1Of	X-100,1
R13220	T17131	T17130	arg1Of	X-100,Triton
R13221	T17132	T17136	arg1Of	",",","
R13222	T17133	T17134	arg1Of	0.1,%
R13223	T17135	T17132	arg2Of	SDS,","
R13224	T17135	T17133	arg1Of	SDS,0.1
R13225	T17136	T17142	arg1Of	",",and
R13226	T17137	T17138	arg1Of	0.5,%
R13227	T17139	T17140	arg1Of	deoxycholate,plus
R13228	T17140	T17136	arg2Of	plus,","
R13229	T17140	T17137	arg1Of	plus,0.5
R13230	T17141	T17140	arg2Of	protease,plus
R13231	T17142	T17115	arg2Of	and,(
R13232	T17144	T17142	arg2Of	inhibitors,and
R13233	T17144	T17143	arg1Of	inhibitors,phosphatase
R13234	T17145	T17115	arg3Of	),(
R13235	T17147	T17146	arg1Of	and,","
R13236	T17148	T17149	arg1Of	chromatin,was
R13237	T17148	T17150	arg2Of	chromatin,sheared
R13238	T17150	T17147	arg2Of	sheared,and
R13239	T17150	T17149	arg2Of	sheared,was
R13240	T17150	T17151	arg1Of	sheared,with
R13241	T17153	T17151	arg2Of	sonicator,with
R13242	T17153	T17152	arg1Of	sonicator,a
R13243	T17153	T17154	modOf	sonicator,to
R13244	T17155	T17154	arg1Of	yield,to
R13245	T17158	T17155	arg2Of	fragments,yield
R13246	T17158	T17156	arg1Of	fragments,0.5–1-kb
R13247	T17158	T17157	arg1Of	fragments,DNA
R13248	T17160	T17168	arg1Of	preclearing,and
R13249	T17163	T17160	arg2Of	chromatin,preclearing
R13250	T17163	T17161	arg1Of	chromatin,the
R13251	T17163	T17162	arg1Of	chromatin,sheared
R13252	T17163	T17164	arg1Of	chromatin,with
R13253	T17167	T17164	arg2Of	beads,with
R13254	T17167	T17165	arg1Of	beads,protein
R13255	T17167	T17166	arg1Of	beads,A–sepharose
R13256	T17168	T17159	arg2Of	and,After
R13257	T17169	T17168	arg2Of	removing,and
R13258	T17169	T17172	arg1Of	removing,as
R13259	T17171	T17169	arg2Of	%,removing
R13260	T17171	T17170	arg1Of	%,5
R13261	T17174	T17172	arg2Of	chromatin,as
R13262	T17174	T17173	arg1Of	chromatin,input
R13263	T17176	T17160	arg1Of	immunoprecipitation,preclearing
R13264	T17176	T17169	arg1Of	immunoprecipitation,removing
R13265	T17176	T17177	arg1Of	immunoprecipitation,was
R13266	T17176	T17178	arg2Of	immunoprecipitation,performed
R13267	T17178	T17159	arg1Of	performed,After
R13268	T17178	T17175	arg1Of	performed,","
R13269	T17178	T17177	arg2Of	performed,was
R13270	T17178	T17179	arg1Of	performed,by
R13271	T17180	T17179	arg2Of	adding,by
R13272	T17183	T17180	arg2Of	amounts,adding
R13273	T17183	T17181	arg2Of	amounts,optimized
R13274	T17183	T17182	arg1Of	amounts,antibody
R13275	T17183	T17184	arg1Of	amounts,(
R13276	T17185	T17184	arg2Of	per,(
R13277	T17190	T17185	arg2Of	equivalents,per
R13278	T17190	T17186	arg1Of	equivalents,20
R13279	T17190	T17187	arg1Of	equivalents,×
R13280	T17190	T17188	arg1Of	equivalents,106
R13281	T17190	T17189	arg1Of	equivalents,cell
R13282	T17191	T17184	arg3Of	:,(
R13283	T17194	T17192	arg1Of	anti-Eomes,2.5
R13284	T17194	T17193	arg1Of	anti-Eomes,μg
R13285	T17194	T17195	arg1Of	anti-Eomes,","
R13286	T17195	T17202	arg1Of	",",","
R13287	T17195	T17203	arg2Of	",",followed
R13288	T17198	T17197	arg1Of	100,:
R13289	T17200	T17195	arg2Of	anti-Runx3,","
R13290	T17200	T17196	arg1Of	anti-Runx3,1
R13291	T17200	T17198	arg1Of	anti-Runx3,100
R13292	T17200	T17199	arg1Of	anti-Runx3,dilution
R13293	T17200	T17201	arg1Of	anti-Runx3,)
R13294	T17203	T17209	arg1Of	followed,;
R13295	T17206	T17203	arg1Of	incubation,followed
R13296	T17206	T17204	arg2Of	incubation,by
R13297	T17206	T17205	arg1Of	incubation,overnight
R13298	T17206	T17207	arg1Of	incubation,at
R13299	T17208	T17207	arg2Of	4°C,at
R13300	T17212	T17210	arg1Of	beads,protein
R13301	T17212	T17211	arg1Of	beads,A–sepharose
R13302	T17212	T17213	arg1Of	beads,were
R13303	T17212	T17214	arg2Of	beads,added
R13304	T17214	T17209	arg2Of	added,;
R13305	T17214	T17213	arg2Of	added,were
R13306	T17214	T17215	arg1Of	added,for
R13307	T17219	T17215	arg2Of	h,for
R13308	T17219	T17216	arg1Of	h,the
R13309	T17219	T17217	arg1Of	h,last
R13310	T17219	T17218	arg1Of	h,3
R13311	T17219	T17220	arg1Of	h,of
R13312	T17223	T17220	arg2Of	period,of
R13313	T17223	T17221	arg1Of	period,the
R13314	T17223	T17222	arg1Of	period,incubation
R13315	T17224	T17225	arg1Of	Beads,were
R13316	T17224	T17226	arg2Of	Beads,washed
R13317	T17226	T17225	arg2Of	washed,were
R13318	T17227	T17228	arg1Of	2×,with
R13319	T17227	T17260	arg1Of	2×,","
R13320	T17230	T17228	arg2Of	buffer,with
R13321	T17230	T17229	arg1Of	buffer,RIPA
R13322	T17230	T17231	arg1Of	buffer,(
R13323	T17232	T17233	arg1Of	50,mM
R13324	T17234	T17232	arg1Of	Tris,50
R13325	T17234	T17235	arg1Of	Tris,[
R13326	T17234	T17239	arg1Of	Tris,","
R13327	T17236	T17235	arg2Of	pH,[
R13328	T17236	T17237	arg1Of	pH,8
R13329	T17238	T17235	arg3Of	],[
R13330	T17239	T17243	arg1Of	",",","
R13331	T17240	T17241	arg1Of	150,mM
R13332	T17242	T17239	arg2Of	NaCl,","
R13333	T17242	T17240	arg1Of	NaCl,150
R13334	T17243	T17247	arg1Of	",",","
R13335	T17244	T17245	arg1Of	1,mM
R13336	T17246	T17243	arg2Of	EDTA,","
R13337	T17246	T17244	arg1Of	EDTA,1
R13338	T17247	T17231	arg2Of	",",(
R13339	T17247	T17251	arg1Of	",",","
R13340	T17248	T17249	arg1Of	1,%
R13341	T17250	T17247	arg2Of	NP-40,","
R13342	T17250	T17248	arg1Of	NP-40,1
R13343	T17252	T17253	arg1Of	0.1,%
R13344	T17254	T17251	arg2Of	SDS,","
R13345	T17254	T17252	arg1Of	SDS,0.1
R13346	T17254	T17255	arg1Of	SDS,","
R13347	T17256	T17257	arg1Of	0.5,%
R13348	T17258	T17255	arg2Of	deoxycholate,","
R13349	T17258	T17256	arg1Of	deoxycholate,0.5
R13350	T17259	T17231	arg3Of	),(
R13351	T17260	T17292	arg1Of	",",and
R13352	T17261	T17260	arg2Of	1×,","
R13353	T17261	T17262	arg1Of	1×,with
R13354	T17265	T17262	arg2Of	buffer,with
R13355	T17265	T17263	arg1Of	buffer,high
R13356	T17265	T17264	arg1Of	buffer,salt
R13357	T17265	T17266	arg1Of	buffer,(
R13358	T17267	T17268	arg1Of	50,mM
R13359	T17269	T17267	arg1Of	Tris,50
R13360	T17269	T17270	arg1Of	Tris,[
R13361	T17269	T17274	arg1Of	Tris,","
R13362	T17271	T17270	arg2Of	pH,[
R13363	T17271	T17272	arg1Of	pH,8
R13364	T17273	T17270	arg3Of	],[
R13365	T17274	T17278	arg1Of	",",","
R13366	T17275	T17276	arg1Of	500,mM
R13367	T17277	T17274	arg2Of	NaCl,","
R13368	T17277	T17275	arg1Of	NaCl,500
R13369	T17278	T17282	arg1Of	",",","
R13370	T17279	T17280	arg1Of	1,mM
R13371	T17281	T17278	arg2Of	EDTA,","
R13372	T17281	T17279	arg1Of	EDTA,1
R13373	T17282	T17266	arg2Of	",",(
R13374	T17282	T17286	arg1Of	",",","
R13375	T17283	T17284	arg1Of	1,%
R13376	T17285	T17282	arg2Of	NP-40,","
R13377	T17285	T17283	arg1Of	NP-40,1
R13378	T17287	T17288	arg1Of	0.1,%
R13379	T17289	T17286	arg2Of	SDS,","
R13380	T17289	T17287	arg1Of	SDS,0.1
R13381	T17290	T17266	arg3Of	),(
R13382	T17292	T17226	arg3Of	and,washed
R13383	T17292	T17291	arg1Of	and,","
R13384	T17293	T17292	arg2Of	1×,and
R13385	T17293	T17294	arg1Of	1×,with
R13386	T17296	T17294	arg2Of	buffer,with
R13387	T17296	T17295	arg1Of	buffer,TE
R13388	T17300	T17297	arg2Of	wash,After
R13389	T17300	T17298	arg1Of	wash,the
R13390	T17300	T17299	arg1Of	wash,last
R13391	T17302	T17303	arg1Of	DNA,was
R13392	T17302	T17304	arg2Of	DNA,eluted
R13393	T17304	T17297	arg1Of	eluted,After
R13394	T17304	T17301	arg1Of	eluted,","
R13395	T17304	T17303	arg2Of	eluted,was
R13396	T17304	T17305	arg1Of	eluted,by
R13397	T17306	T17305	arg2Of	resuspending,by
R13398	T17308	T17306	arg2Of	beads,resuspending
R13399	T17308	T17307	arg1Of	beads,the
R13400	T17308	T17309	arg1Of	beads,in
R13401	T17311	T17309	arg2Of	buffer,in
R13402	T17311	T17310	arg1Of	buffer,elution
R13403	T17311	T17312	arg1Of	buffer,(
R13404	T17313	T17314	arg1Of	1,%
R13405	T17315	T17312	arg2Of	SDS,(
R13406	T17315	T17313	arg1Of	SDS,1
R13407	T17315	T17316	arg1Of	SDS,","
R13408	T17317	T17318	arg1Of	100,mM
R13409	T17319	T17316	arg2Of	NaHCO3,","
R13410	T17319	T17317	arg1Of	NaHCO3,100
R13411	T17320	T17312	arg3Of	),(
R13412	T17322	T17323	arg1Of	input,and
R13413	T17323	T17321	arg1Of	and,Both
R13414	T17323	T17326	arg1Of	and,were
R13415	T17323	T17328	arg2Of	and,treated
R13416	T17325	T17323	arg2Of	chromatin,and
R13417	T17325	T17324	arg1Of	chromatin,ChIP
R13418	T17328	T17326	arg2Of	treated,were
R13419	T17328	T17327	arg1Of	treated,then
R13420	T17328	T17329	arg1Of	treated,with
R13421	T17328	T17337	arg1Of	treated,for
R13422	T17328	T17342	arg1Of	treated,","
R13423	T17328	T17343	modOf	treated,followed
R13424	T17331	T17329	arg2Of	A,with
R13425	T17331	T17330	arg1Of	A,RNase
R13426	T17331	T17332	arg1Of	A,(
R13427	T17335	T17332	arg2Of	total,(
R13428	T17335	T17333	arg1Of	total,5
R13429	T17335	T17334	arg1Of	total,μg
R13430	T17336	T17332	arg3Of	),(
R13431	T17339	T17337	arg2Of	h,for
R13432	T17339	T17338	arg1Of	h,1
R13433	T17339	T17340	arg1Of	h,at
R13434	T17341	T17340	arg2Of	37°C,at
R13435	T17346	T17343	arg1Of	addition,followed
R13436	T17346	T17344	arg2Of	addition,by
R13437	T17346	T17345	arg1Of	addition,the
R13438	T17346	T17347	arg1Of	addition,of
R13439	T17349	T17348	arg1Of	K,proteinase
R13440	T17349	T17350	arg1Of	K,(
R13441	T17349	T17355	arg1Of	K,and
R13442	T17353	T17350	arg2Of	total,(
R13443	T17353	T17351	arg1Of	total,100
R13444	T17353	T17352	arg1Of	total,μg
R13445	T17354	T17350	arg3Of	),(
R13446	T17355	T17347	arg2Of	and,of
R13447	T17357	T17355	arg2Of	incubation,and
R13448	T17357	T17356	arg1Of	incubation,overnight
R13449	T17357	T17358	arg1Of	incubation,at
R13450	T17357	T17360	modOf	incubation,to
R13451	T17359	T17358	arg2Of	65°C,at
R13452	T17361	T17360	arg1Of	reverse,to
R13453	T17362	T17361	arg2Of	cross-linking,reverse
R13454	T17363	T17364	arg1Of	DNA,was
R13455	T17363	T17366	arg2Of	DNA,purified
R13456	T17363	T17384	arg1Of	DNA,resuspended
R13457	T17366	T17367	arg1Of	purified,with
R13458	T17366	T17377	arg1Of	purified,according
R13459	T17366	T17383	arg1Of	purified,and
R13460	T17369	T17367	arg2Of	columns,with
R13461	T17369	T17368	arg1Of	columns,QIAquick
R13462	T17369	T17370	arg1Of	columns,(
R13463	T17373	T17370	arg2Of	Kit,(
R13464	T17373	T17371	arg1Of	Kit,Gel
R13465	T17373	T17372	arg1Of	Kit,Extraction
R13466	T17373	T17374	arg1Of	Kit,;
R13467	T17375	T17374	arg2Of	QIAGEN,;
R13468	T17376	T17370	arg3Of	),(
R13469	T17378	T17377	arg2Of	to,according
R13470	T17380	T17379	arg1Of	manufacturer,the
R13471	T17380	T17381	arg2Of	manufacturer,'s
R13472	T17382	T17378	arg2Of	instructions,to
R13473	T17382	T17381	arg1Of	instructions,'s
R13474	T17383	T17364	arg2Of	and,was
R13475	T17383	T17365	arg1Of	and,then
R13476	T17384	T17383	arg2Of	resuspended,and
R13477	T17384	T17385	arg1Of	resuspended,in
R13478	T17388	T17385	arg2Of	volume,in
R13479	T17388	T17386	arg1Of	volume,a
R13480	T17388	T17387	arg1Of	volume,50-μl
R13481	T17392	T17389	arg2Of	detection,For
R13482	T17392	T17390	arg1Of	detection,real-time
R13483	T17392	T17391	arg1Of	detection,PCR
R13484	T17392	T17393	arg1Of	detection,of
R13485	T17395	T17393	arg2Of	targets,of
R13486	T17395	T17394	arg1Of	targets,immunoprecipitated
R13487	T17395	T17396	arg1Of	targets,using
R13488	T17399	T17398	arg1Of	Green,SYBR
R13489	T17401	T17396	arg2Of	Kit,using
R13490	T17401	T17397	arg1Of	Kit,the
R13491	T17401	T17399	arg1Of	Kit,Green
R13492	T17401	T17400	arg1Of	Kit,PCR
R13493	T17405	T17403	arg1Of	curve,a
R13494	T17405	T17404	arg1Of	curve,standard
R13495	T17405	T17406	arg1Of	curve,was
R13496	T17405	T17407	arg2Of	curve,obtained
R13497	T17407	T17406	arg2Of	obtained,was
R13498	T17407	T17408	arg1Of	obtained,with
R13499	T17407	T17418	arg1Of	obtained,and
R13500	T17410	T17408	arg2Of	dilutions,with
R13501	T17410	T17409	arg1Of	dilutions,serial
R13502	T17410	T17411	arg1Of	dilutions,of
R13503	T17413	T17411	arg2Of	DNA,of
R13504	T17413	T17412	arg1Of	DNA,input
R13505	T17413	T17414	arg1Of	DNA,for
R13506	T17416	T17414	arg2Of	sample,for
R13507	T17416	T17415	arg1Of	sample,each
R13508	T17418	T17389	arg1Of	and,For
R13509	T17418	T17402	arg1Of	and,","
R13510	T17418	T17417	arg1Of	and,","
R13511	T17422	T17419	arg1Of	DNA,1
R13512	T17422	T17420	arg1Of	DNA,μl
R13513	T17422	T17421	arg1Of	DNA,ChIP
R13514	T17422	T17423	arg1Of	DNA,was
R13515	T17422	T17424	arg2Of	DNA,used
R13516	T17424	T17418	arg2Of	used,and
R13517	T17424	T17423	arg2Of	used,was
R13518	T17424	T17425	arg1Of	used,per
R13519	T17427	T17425	arg2Of	reaction,per
R13520	T17427	T17426	arg1Of	reaction,PCR
R13521	T17427	T17428	arg1Of	reaction,(
R13522	T17429	T17428	arg2Of	performed,(
R13523	T17429	T17430	arg1Of	performed,in
R13524	T17431	T17430	arg2Of	duplicates,in
R13525	T17432	T17428	arg3Of	),(
R13526	T17434	T17433	arg1Of	curves,Melt
R13527	T17434	T17435	arg1Of	curves,and
R13528	T17435	T17438	arg1Of	and,were
R13529	T17435	T17439	arg2Of	and,analyzed
R13530	T17437	T17435	arg2Of	gels,and
R13531	T17437	T17436	arg1Of	gels,agarose
R13532	T17439	T17438	arg2Of	analyzed,were
R13533	T17439	T17440	modOf	analyzed,to
R13534	T17441	T17440	arg1Of	ensure,to
R13535	T17442	T17441	arg2Of	amplification,ensure
R13536	T17442	T17443	arg1Of	amplification,of
R13537	T17446	T17443	arg2Of	sequences,of
R13538	T17446	T17444	arg1Of	sequences,specific
R13539	T17446	T17445	arg1Of	sequences,target
R13540	T17447	T17458	arg1Of	Refer,for
R13541	T17450	T17447	arg2Of	S1,Refer
R13542	T17450	T17448	arg1Of	S1,to
R13543	T17450	T17449	arg1Of	S1,Table
R13544	T17450	T17451	arg1Of	S1,(
R13545	T17452	T17451	arg2Of	available,(
R13546	T17452	T17453	arg1Of	available,at
R13547	T17454	T17453	arg2Of	http,at
R13548	T17454	T17455	arg1Of	http,:
R13549	T17456	T17455	arg2Of	//www.jem.org/cgi/content/full/jem.20081242/DC1,:
R13550	T17457	T17451	arg3Of	),(
R13551	T17460	T17458	arg2Of	list,for
R13552	T17460	T17459	arg1Of	list,a
R13553	T17460	T17461	arg1Of	list,of
R13554	T17463	T17461	arg2Of	sets,of
R13555	T17463	T17462	arg1Of	sets,primer
R13556	T17465	T17464	arg1Of	data,The
R13557	T17465	T17466	arg1Of	data,are
R13558	T17465	T17467	arg2Of	data,presented
R13559	T17467	T17466	arg2Of	presented,are
R13560	T17467	T17468	arg1Of	presented,as
R13561	T17467	T17479	arg1Of	presented,","
R13562	T17467	T17480	modOf	presented,assuming
R13563	T17470	T17468	arg2Of	number,as
R13564	T17470	T17469	arg1Of	number,the
R13565	T17470	T17471	arg1Of	number,of
R13566	T17474	T17471	arg2Of	sequences,of
R13567	T17474	T17472	arg2Of	sequences,immunoprecipitated
R13568	T17474	T17473	arg1Of	sequences,target
R13569	T17474	T17475	arg1Of	sequences,relative
R13570	T17475	T17476	arg1Of	relative,to
R13571	T17478	T17476	arg2Of	chromatin,to
R13572	T17478	T17477	arg1Of	chromatin,input
R13573	T17482	T17480	arg2Of	copies,assuming
R13574	T17482	T17481	arg1Of	copies,two
R13575	T17482	T17483	arg1Of	copies,of
R13576	T17482	T17486	arg1Of	copies,per
R13577	T17485	T17483	arg2Of	sequence,of
R13578	T17485	T17484	arg1Of	sequence,target
R13579	T17487	T17486	arg2Of	cell,per
R13580	T17487	T17488	arg1Of	cell,equivalent
R13581	T17487	T17489	arg2Of	cell,used
R13582	T17489	T17490	arg1Of	used,for
R13583	T17492	T17490	arg2Of	ChIP,for
R13584	T17492	T17491	arg1Of	ChIP,the
R13874	T17860	T17856	arg2Of	and,was
R13875	T17861	T17860	arg2Of	transferred,and
R13876	T17861	T17862	arg1Of	transferred,to
R13877	T17864	T17863	arg1Of	charged,positively
R13878	T17866	T17862	arg2Of	membranes,to
R13879	T17866	T17864	arg1Of	membranes,charged
R13880	T17866	T17865	arg1Of	membranes,nylon
R13881	T17866	T17867	arg1Of	membranes,(
R13882	T17866	T17873	arg1Of	membranes,","
R13883	T17866	T17874	arg1Of	membranes,which
R13884	T17866	T17875	arg1Of	membranes,was
R13885	T17866	T17876	arg2Of	membranes,confirmed
R13886	T17868	T17869	arg1Of	Hybond-N+,;
R13887	T17869	T17867	arg2Of	;,(
R13888	T17871	T17869	arg2Of	Healthcare,;
R13889	T17871	T17870	arg1Of	Healthcare,GE
R13890	T17872	T17867	arg3Of	),(
R13891	T17876	T17875	arg2Of	confirmed,was
R13892	T17880	T17876	arg1Of	staining,confirmed
R13893	T17880	T17877	arg2Of	staining,by
R13894	T17880	T17878	arg1Of	staining,ethidium
R13895	T17880	T17879	arg1Of	staining,bromide
R13896	T17880	T17881	arg1Of	staining,of
R13897	T17880	T17885	arg1Of	staining,on
R13898	T17884	T17881	arg2Of	species,of
R13899	T17884	T17882	arg1Of	species,ribosomal
R13900	T17884	T17883	arg1Of	species,RNA
R13901	T17887	T17885	arg2Of	membrane,on
R13902	T17887	T17886	arg1Of	membrane,the
R13903	T17888	T17889	arg1Of	Membranes,were
R13904	T17888	T17890	arg2Of	Membranes,hybridized
R13905	T17890	T17889	arg2Of	hybridized,were
R13906	T17890	T17891	arg1Of	hybridized,with
R13907	T17892	T17893	arg1Of	1,ng/ml
R13908	T17894	T17895	arg1Of	α-,[
R13909	T17896	T17895	arg2Of	32P,[
R13910	T17897	T17895	arg3Of	],[
R13911	T17898	T17894	arg1Of	dCTP–labeled,α-
R13912	T17902	T17891	arg2Of	probe,with
R13913	T17902	T17892	arg1Of	probe,1
R13914	T17902	T17898	arg1Of	probe,dCTP–labeled
R13915	T17902	T17899	arg1Of	probe,trichloroacetic
R13916	T17902	T17900	arg1Of	probe,acid
R13917	T17902	T17901	arg1Of	probe,precipitable
R13918	T17902	T17903	arg1Of	probe,in
R13919	T17906	T17903	arg2Of	buffer,in
R13920	T17906	T17904	arg1Of	buffer,ExpressHyb
R13921	T17906	T17905	arg1Of	buffer,hybridization
R13922	T17906	T17907	arg1Of	buffer,(
R13923	T17909	T17907	arg2Of	Laboratories,(
R13924	T17909	T17908	arg1Of	Laboratories,Clontech
R13925	T17909	T17910	arg1Of	Laboratories,","
R13926	T17911	T17910	arg2Of	Inc.,","
R13927	T17912	T17907	arg3Of	),(
R13928	T17915	T17913	arg1Of	probes,All
R13929	T17915	T17914	arg1Of	probes,cDNA
R13930	T17915	T17916	arg1Of	probes,were
R13931	T17915	T17917	arg2Of	probes,confirmed
R13932	T17915	T17919	arg1Of	probes,have
R13933	T17917	T17916	arg2Of	confirmed,were
R13934	T17919	T17917	arg3Of	have,confirmed
R13935	T17919	T17918	arg1Of	have,to
R13936	T17919	T17924	arg1Of	have,under
R13937	T17923	T17919	arg2Of	specificity,have
R13938	T17923	T17920	arg1Of	specificity,the
R13939	T17923	T17921	arg1Of	specificity,appropriate
R13940	T17923	T17922	arg1Of	specificity,single-copy
R13941	T17926	T17924	arg2Of	conditions,under
R13942	T17926	T17925	arg1Of	conditions,these
R13943	T17926	T17927	arg1Of	conditions,using
R13944	T17931	T17927	arg2Of	analysis,using
R13945	T17931	T17928	arg1Of	analysis,genomic
R13946	T17931	T17929	arg1Of	analysis,Southern
R13947	T17931	T17930	arg1Of	analysis,blot
R13948	T17933	T17932	arg1Of	intensities,Band
R13949	T17933	T17934	arg1Of	intensities,were
R13950	T17933	T17935	arg2Of	intensities,acquired
R13951	T17935	T17934	arg2Of	acquired,were
R13952	T17935	T17936	arg1Of	acquired,by
R13953	T17937	T17936	arg2Of	phosphorimaging,by
R13954	T17938	T17937	arg2Of	analysis,phosphorimaging
R13955	T17941	T17939	arg2Of	analysis,For
R13956	T17941	T17940	arg1Of	analysis,Western
R13957	T17945	T17943	arg1Of	lysates,whole-cell
R13958	T17945	T17944	arg1Of	lysates,protein
R13959	T17945	T17946	arg1Of	lysates,were
R13960	T17945	T17947	arg2Of	lysates,obtained
R13961	T17945	T17996	arg1Of	lysates,resuspending
R13962	T17945	T18005	arg1Of	lysates,incubating
R13963	T17947	T17939	arg1Of	obtained,For
R13964	T17947	T17942	arg1Of	obtained,","
R13965	T17947	T17946	arg2Of	obtained,were
R13966	T17947	T17948	arg1Of	obtained,from
R13967	T17947	T17952	arg1Of	obtained,at
R13968	T17947	T17960	arg1Of	obtained,during
R13969	T17947	T17995	arg1Of	obtained,by
R13970	T17951	T17948	arg2Of	cells,from
R13971	T17951	T17949	arg1Of	cells,CD8+
R13972	T17951	T17950	arg1Of	cells,T
R13973	T17954	T17952	arg2Of	time,at
R13974	T17954	T17953	arg1Of	time,the
R13975	T17955	T17956	arg1Of	points,indicated
R13976	T17956	T17954	arg2Of	indicated,time
R13977	T17956	T17957	arg1Of	indicated,in
R13978	T17959	T17957	arg2Of	figures,in
R13979	T17959	T17958	arg1Of	figures,the
R13980	T17962	T17960	arg2Of	expansion,during
R13981	T17962	T17961	arg1Of	expansion,clonal
R13982	T17962	T17963	arg1Of	expansion,in
R13983	T17962	T17967	arg1Of	expansion,with
R13984	T17964	T17965	arg1Of	100,U/ml
R13985	T17966	T17963	arg2Of	IL-2,in
R13986	T17966	T17964	arg1Of	IL-2,100
R13987	T17969	T17967	arg2Of	buffer,with
R13988	T17969	T17968	arg1Of	buffer,lysis
R13989	T17969	T17970	arg1Of	buffer,(
R13990	T17971	T17972	arg1Of	50,mM
R13991	T17973	T17971	arg1Of	Tris,50
R13992	T17973	T17974	arg1Of	Tris,[
R13993	T17973	T17978	arg1Of	Tris,","
R13994	T17975	T17974	arg2Of	pH,[
R13995	T17975	T17976	arg1Of	pH,7.5
R13996	T17977	T17974	arg3Of	],[
R13997	T17978	T17970	arg2Of	",",(
R13998	T17979	T17980	arg1Of	150,mM
R13999	T17981	T17979	arg1Of	NaCl,150
R14000	T17981	T17982	arg1Of	NaCl,","
R14001	T17982	T17986	arg1Of	",",","
R14002	T17983	T17984	arg1Of	10,%
R14003	T17985	T17982	arg2Of	glycerol,","
R14004	T17985	T17983	arg1Of	glycerol,10
R14005	T17986	T17990	arg1Of	",",","
R14006	T17987	T17988	arg1Of	5,mM
R14007	T17989	T17986	arg2Of	EDTA,","
R14008	T17989	T17987	arg1Of	EDTA,5
R14009	T17990	T17978	arg2Of	",",","
R14010	T17991	T17992	arg1Of	1,%
R14011	T17993	T17990	arg2Of	NP-40,","
R14012	T17993	T17991	arg1Of	NP-40,1
R14013	T17994	T17970	arg3Of	),(
R14014	T17996	T17998	arg1Of	resuspending,in
R14015	T17996	T18004	arg1Of	resuspending,and
R14016	T17997	T17996	arg2Of	samples,resuspending
R14017	T18000	T17998	arg2Of	μl,in
R14018	T18000	T17999	arg1Of	μl,10
R14019	T18000	T18001	arg1Of	μl,per
R14020	T18003	T18001	arg2Of	cells,per
R14021	T18003	T18002	arg1Of	cells,106
R14022	T18004	T17995	arg2Of	and,by
R14023	T18005	T18004	arg2Of	incubating,and
R14024	T18005	T18006	arg1Of	incubating,on
R14025	T18005	T18008	arg1Of	incubating,for
R14026	T18005	T18011	arg1Of	incubating,in
R14027	T18007	T18006	arg2Of	ice,on
R14028	T18010	T18008	arg2Of	min,for
R14029	T18010	T18009	arg1Of	min,30
R14030	T18013	T18011	arg2Of	presence,in
R14031	T18013	T18012	arg1Of	presence,the
R14032	T18013	T18014	arg1Of	presence,of
R14033	T18016	T18014	arg2Of	inhibitors,of
R14034	T18016	T18015	arg1Of	inhibitors,protease
R14035	T18018	T18017	arg1Of	analysis,Immunoblot
R14036	T18018	T18019	arg1Of	analysis,was
R14037	T18018	T18020	arg2Of	analysis,performed
R14038	T18020	T18019	arg2Of	performed,was
R14039	T18020	T18021	arg1Of	performed,with
R14040	T18023	T18021	arg2Of	antibodies,with
R14041	T18023	T18022	arg1Of	antibodies,the
R14042	T18023	T18024	arg2Of	antibodies,indicated
R14043	T18024	T18025	arg1Of	indicated,in
R14044	T18024	T18028	arg1Of	indicated,after
R14045	T18027	T18025	arg2Of	figures,in
R14046	T18027	T18026	arg1Of	figures,the
R14047	T18029	T18028	arg2Of	SDS-PAGE,after
R14048	T18029	T18030	arg1Of	SDS-PAGE,(
R14049	T18032	T18031	arg1Of	μg,10–30
R14050	T18032	T18033	arg1Of	μg,of
R14051	T18032	T18036	arg1Of	μg,was
R14052	T18032	T18037	arg2Of	μg,loaded
R14053	T18035	T18033	arg2Of	protein,of
R14054	T18035	T18034	arg1Of	protein,total
R14055	T18037	T18030	arg2Of	loaded,(
R14056	T18037	T18036	arg2Of	loaded,was
R14057	T18037	T18038	arg1Of	loaded,per
R14058	T18039	T18038	arg2Of	well,per
R14059	T18040	T18030	arg3Of	),(
R14060	T18041	T18042	arg1Of	Quantification,of
R14061	T18041	T18045	arg1Of	Quantification,was
R14062	T18041	T18046	arg2Of	Quantification,performed
R14063	T18041	T18059	arg2Of	Quantification,normalized
R14064	T18041	T18061	arg1Of	Quantification,loading
R14065	T18044	T18042	arg2Of	protein,of
R14066	T18044	T18043	arg2Of	protein,detected
R14067	T18046	T18047	arg1Of	performed,with
R14068	T18046	T18058	arg1Of	performed,and
R14069	T18051	T18049	arg1Of	Box,Intelligent
R14070	T18051	T18050	arg1Of	Box,Dark
R14071	T18052	T18047	arg2Of	unit,with
R14072	T18052	T18048	arg1Of	unit,an
R14073	T18052	T18051	arg1Of	unit,Box
R14074	T18052	T18053	arg1Of	unit,(
R14075	T18054	T18053	arg2Of	LAS-3000,(
R14076	T18054	T18055	arg1Of	LAS-3000,;
R14077	T18056	T18055	arg2Of	Fujifilm,;
R14078	T18057	T18053	arg3Of	),(
R14079	T18058	T18045	arg2Of	and,was
R14080	T18059	T18058	arg2Of	normalized,and
R14081	T18059	T18060	arg1Of	normalized,for
R14082	T18061	T18060	arg2Of	loading,for
R14083	T18061	T18062	arg1Of	loading,with
R14084	T18064	T18062	arg2Of	amount,with
R14085	T18064	T18063	arg1Of	amount,the
R14086	T18064	T18065	arg1Of	amount,of
R14087	T18067	T18065	arg2Of	Pol-II,of
R14088	T18067	T18066	arg1Of	Pol-II,RNA
R14089	T18067	T18068	arg2Of	Pol-II,detected
R14090	T18068	T18069	arg1Of	detected,in
R14091	T18071	T18069	arg2Of	lane,in
R14092	T18071	T18070	arg1Of	lane,each
R14158	T18192	T18190	arg1Of	material,Online
R14159	T18192	T18191	arg1Of	material,supplemental
R14160	T18194	T18193	arg1Of	S1,Fig.
R14161	T18194	T18195	arg1Of	S1,shows
R14162	T18197	T18195	arg2Of	characterization,shows
R14163	T18197	T18196	arg1Of	characterization,the
R14164	T18197	T18198	arg1Of	characterization,of
R14165	T18202	T18198	arg2Of	cells,of
R14166	T18202	T18199	arg1Of	cells,peripheral
R14167	T18202	T18200	arg1Of	cells,CD8+
R14168	T18202	T18201	arg1Of	cells,T
R14169	T18202	T18203	arg1Of	cells,from
R14170	T18205	T18203	arg2Of	mice,from
R14171	T18205	T18204	arg1Of	mice,Runx3−/−
R14172	T18207	T18206	arg1Of	S2,Fig.
R14173	T18207	T18208	arg1Of	S2,shows
R14174	T18211	T18208	arg2Of	expression,shows
R14175	T18211	T18209	arg1Of	expression,effector
R14176	T18211	T18210	arg1Of	expression,protein
R14177	T18211	T18212	arg1Of	expression,by
R14178	T18214	T18213	arg1Of	WT,Runx3
R14179	T18214	T18215	arg1Of	WT,and
R14180	T18215	T18212	arg2Of	and,by
R14181	T18215	T18218	arg1Of	and,at
R14182	T18217	T18215	arg2Of	cells,and
R14183	T18217	T18216	arg1Of	cells,KO
R14184	T18219	T18218	arg2Of	day,at
R14185	T18219	T18220	arg1Of	day,4
R14186	T18219	T18221	arg1Of	day,of
R14187	T18223	T18222	arg1Of	vitro,in
R14188	T18224	T18221	arg2Of	culture,of
R14189	T18224	T18223	arg1Of	culture,vitro
R14190	T18226	T18225	arg1Of	sequences,Primer
R14191	T18226	T18227	arg2Of	sequences,used
R14192	T18226	T18231	arg1Of	sequences,are
R14193	T18226	T18232	arg2Of	sequences,shown
R14194	T18227	T18228	arg1Of	used,for
R14195	T18230	T18228	arg2Of	experiments,for
R14196	T18230	T18229	arg1Of	experiments,ChIP
R14197	T18232	T18231	arg2Of	shown,are
R14198	T18232	T18233	arg1Of	shown,in
R14199	T18235	T18233	arg2Of	S1,in
R14200	T18235	T18234	arg1Of	S1,Table
R14203	T18238	T18237	arg1Of	material,supplemental
R14209	T18244	T18236	arg1Of	//www.jem.org/cgi/content/full/jem.20081242/DC1,Online
R12675	T16516	T16515	arg1Of	assess,To
R12676	T16518	T16516	arg2Of	production,assess
R12677	T16518	T16517	arg1Of	production,cytokine
R12678	T16520	T16516	arg1Of	cells,assess
R12934	T16794	T16793	arg1Of	experiments,transduction
R12939	T16799	T16795	arg1Of	generated,","
R13842	T17829	T17830	arg1Of	Northern,and
R13843	T17831	T17830	arg2Of	Western,and
R13844	T17833	T17829	arg1Of	analyses,Northern
R13845	T17833	T17831	arg1Of	analyses,Western
R13846	T17833	T17832	arg1Of	analyses,blot
R13847	T17835	T17834	arg1Of	isolation,RNA
R13848	T17835	T17844	arg2Of	isolation,described
R13849	T17839	T17836	arg1Of	analysis,and
R13850	T17839	T17837	arg1Of	analysis,Northern
R13851	T17839	T17838	arg1Of	analysis,blot
R13852	T17839	T17840	arg1Of	analysis,was
R13853	T17839	T17841	arg2Of	analysis,performed
R13854	T17841	T17840	arg2Of	performed,was
R13855	T17841	T17842	arg1Of	performed,as
R13856	T17842	T17843	arg1Of	as,previously
R13857	T17844	T17840	modOf	described,was
R13858	T17844	T17845	arg1Of	described,(
R13859	T17846	T17845	arg2Of	29,(
R13860	T17847	T17845	arg3Of	),(
R13861	T17849	T17848	arg2Of	brief,In
R13862	T17852	T17851	arg1Of	μg,10
R13863	T17852	T17853	arg1Of	μg,of
R13864	T17852	T17856	arg1Of	μg,was
R13865	T17852	T17857	arg2Of	μg,loaded
R13866	T17852	T17861	arg2Of	μg,transferred
R13867	T17855	T17853	arg2Of	RNA,of
R13868	T17855	T17854	arg1Of	RNA,total
R13869	T17857	T17858	arg1Of	loaded,per
R13870	T17857	T17860	arg1Of	loaded,and
R13871	T17859	T17858	arg2Of	lane,per
R13872	T17860	T17848	arg1Of	and,In
R13873	T17860	T17850	arg1Of	and,","
R14201	T18236	T18239	modOf	Online,is
R14202	T18236	T18243	arg1Of	Online,:
R14204	T18238	T18239	arg1Of	material,is
R14205	T18238	T18240	arg1Of	material,available
R14206	T18240	T18239	arg2Of	available,is
R14207	T18240	T18241	arg1Of	available,at
R14208	T18242	T18241	arg2Of	http,at

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T13830	23-33	NNS	denotes	Antibodies
T13831	34-37	CC	denotes	and
T13832	38-46	NNS	denotes	reagents
T13833	46-47	.	denotes	.
T13834	48-51	DT	denotes	The
T13835	52-61	VBG	denotes	following
T13836	62-72	NNS	denotes	antibodies
T13837	73-77	VBN	denotes	used
T13838	78-81	IN	denotes	for
T13839	82-95	JJ	denotes	intracellular
T13840	96-98	CC	denotes	or
T13841	99-106	NN	denotes	surface
T13842	107-113	NNS	denotes	stains
T13843	114-118	VBD	denotes	were
T13844	119-127	VBN	denotes	obtained
T13845	128-132	IN	denotes	from
T13846	133-144	NNP	denotes	eBioscience
T13847	144-145	:	denotes	:
T13848	146-150	NNS	denotes	anti
T13849	151-155	NN	denotes	IL-2
T13850	155-156	,	denotes	,
T13851	157-161	NNS	denotes	anti
T13852	162-167	JJ	denotes	IFN-γ
T13853	167-168	,	denotes	,
T13854	169-177	JJ	denotes	anti-TNF
T13855	177-178	,	denotes	,
T13856	179-183	NNS	denotes	anti
T13857	184-192	NN	denotes	granzyme
T13858	193-194	NN	denotes	B
T13859	194-195	,	denotes	,
T13860	196-204	JJ	denotes	anti-CD8
T13861	204-205	,	denotes	,
T13862	206-215	JJ	denotes	anti-CD25
T13863	215-216	,	denotes	,
T13864	217-220	CC	denotes	and
T13865	221-230	JJ	denotes	anti-CD44
T13866	230-231	.	denotes	.
T13867	232-241	NN	denotes	Anti-CD69
T13868	242-245	VBD	denotes	was
T13869	246-255	VBN	denotes	purchased
T13870	256-260	IN	denotes	from
T13871	261-263	NNP	denotes	BD
T13872	263-264	.	denotes	.
T13873	265-268	IN	denotes	For
T13874	269-273	NNP	denotes	ChIP
T13875	274-285	NNS	denotes	experiments
T13876	285-286	,	denotes	,
T13877	287-290	DT	denotes	the
T13878	291-301	JJ	denotes	anti-Eomes
T13879	302-310	NN	denotes	antibody
T13880	311-314	VBD	denotes	was
T13881	315-323	VBN	denotes	obtained
T13882	324-328	IN	denotes	from
T13883	329-334	NNP	denotes	Abcam
T13884	335-338	CC	denotes	and
T13885	339-342	DT	denotes	the
T13886	343-353	JJ	denotes	anti-Runx3
T13887	354-362	NN	denotes	antibody
T13888	363-366	VBD	denotes	was
T13889	367-375	VBN	denotes	produced
T13890	376-378	IN	denotes	by
T13891	379-382	DT	denotes	the
T13892	383-389	NNP	denotes	Groner
T13893	390-400	NN	denotes	laboratory
T13894	400-401	.	denotes	.
T13895	402-405	DT	denotes	The
T13896	406-415	VBG	denotes	following
T13897	416-426	NNS	denotes	antibodies
T13898	427-431	VBD	denotes	were
T13899	432-436	VBN	denotes	used
T13900	437-440	IN	denotes	for
T13901	441-455	VBG	denotes	immunoblotting
T13902	455-456	:	denotes	:
T13903	457-469	NN	denotes	antiperforin
T13904	470-471	-LRB-	denotes	(
T13905	471-476	NNP	denotes	Abcam
T13906	476-477	-RRB-	denotes	)
T13907	477-478	,	denotes	,
T13908	479-489	JJ	denotes	anti-Eomes
T13909	490-491	-LRB-	denotes	(
T13910	491-496	NNP	denotes	Abcam
T13911	496-497	-RRB-	denotes	)
T13912	497-498	,	denotes	,
T13913	499-502	CC	denotes	and
T13914	503-507	NNS	denotes	anti
T13915	508-514	NNP	denotes	Pol-II
T13916	515-516	-LRB-	denotes	(
T13917	516-521	NNP	denotes	Santa
T13918	522-526	NNP	denotes	Cruz
T13919	527-540	NNP	denotes	Biotechnology
T13920	540-541	,	denotes	,
T13921	542-546	NNP	denotes	Inc.
T13922	546-547	-RRB-	denotes	)
T13923	547-548	.	denotes	.
T13924	549-552	DT	denotes	The
T13925	553-558	NN	denotes	T-bet
T13926	559-567	NN	denotes	antibody
T13927	568-571	VBD	denotes	was
T13928	572-580	VBN	denotes	provided
T13929	581-583	IN	denotes	by
T13930	584-586	NNP	denotes	L.
T13931	587-595	NNP	denotes	Glimcher
T13932	596-597	-LRB-	denotes	(
T13933	597-604	NNP	denotes	Harvard
T13934	605-611	NNP	denotes	School
T13935	612-614	IN	denotes	of
T13936	615-621	NNP	denotes	Public
T13937	622-628	NNP	denotes	Health
T13938	628-629	,	denotes	,
T13939	630-636	NNP	denotes	Boston
T13940	636-637	,	denotes	,
T13941	638-640	NNP	denotes	MA
T13942	640-641	-RRB-	denotes	)
T13943	641-642	.	denotes	.
T13944	643-646	DT	denotes	The
T13945	647-656	VBG	denotes	following
T13946	657-665	NNS	denotes	reagents
T13947	666-670	VBD	denotes	were
T13948	671-675	VBN	denotes	used
T13949	676-679	IN	denotes	for
T13950	680-683	DT	denotes	the
T13951	684-695	NNS	denotes	experiments
T13952	696-705	VBN	denotes	presented
T13953	706-708	IN	denotes	in
T13954	709-713	DT	denotes	this
T13955	714-720	NN	denotes	report
T13956	720-721	:	denotes	:
T13957	722-729	NNP	denotes	Annexin
T13958	730-731	NNP	denotes	V
T13959	732-736	NNP	denotes	FITC
T13960	737-746	NNP	denotes	Apoptosis
T13961	747-756	NNP	denotes	Detection
T13962	757-760	NNP	denotes	Kit
T13963	761-762	-LRB-	denotes	(
T13964	762-764	NNP	denotes	BD
T13965	764-765	-RRB-	denotes	)
T13966	765-766	,	denotes	,
T13967	767-770	NNP	denotes	CD8
T13968	771-779	JJ	denotes	Negative
T13969	780-789	NNP	denotes	Isolation
T13970	790-793	NNP	denotes	Kit
T13971	794-795	-LRB-	denotes	(
T13972	795-805	NNP	denotes	Invitrogen
T13973	805-806	-RRB-	denotes	)
T13974	806-807	,	denotes	,
T13975	808-811	NNP	denotes	CD8
T13976	812-822	NNP	denotes	MicroBeads
T13977	823-824	-LRB-	denotes	(
T13978	824-832	NNP	denotes	Miltenyi
T13979	833-839	NNP	denotes	Biotec
T13980	839-840	-RRB-	denotes	)
T13981	840-841	,	denotes	,
T13982	842-845	CC	denotes	and
T13983	846-850	NNP	denotes	SYBR
T13984	851-856	NNP	denotes	Green
T13985	857-860	NNP	denotes	PCR
T13986	861-865	NNP	denotes	Core
T13987	866-874	NNP	denotes	Reagents
T13988	875-876	-LRB-	denotes	(
T13989	876-883	NNP	denotes	Applied
T13990	884-894	NNPS	denotes	Biosystems
T13991	894-895	-RRB-	denotes	)
T13992	895-896	.	denotes	.
T13993	897-900	DT	denotes	The
T13994	901-905	JJ	denotes	Gp33
T13995	906-913	NN	denotes	peptide
T13996	914-915	-LRB-	denotes	(
T13997	915-924	NNP	denotes	KAVYNFATC
T13998	924-925	-RRB-	denotes	)
T13999	926-929	VBD	denotes	was
T14000	930-941	VBN	denotes	synthesized
T14001	942-944	IN	denotes	by
T14002	945-948	DT	denotes	the
T14003	949-954	NNP	denotes	Tufts
T14004	955-965	NNP	denotes	University
T14005	966-970	NNP	denotes	Core
T14006	971-979	NNP	denotes	Facility
T14007	979-980	,	denotes	,
T14008	981-984	CC	denotes	and
T14009	985-987	CD	denotes	10
T14010	988-990	NN	denotes	mM
T14011	991-993	IN	denotes	of
T14012	994-999	NN	denotes	stock
T14013	1000-1009	NNS	denotes	solutions
T14014	1010-1013	VBD	denotes	was
T14015	1014-1022	VBN	denotes	prepared
T14016	1023-1025	IN	denotes	in
T14017	1026-1030	NNP	denotes	DMSO
T14018	1030-1031	.	denotes	.
T14382	1033-1042	NN	denotes	Isolation
T14383	1043-1046	CC	denotes	and
T14384	1047-1054	NN	denotes	culture
T14385	1055-1057	IN	denotes	of
T14386	1058-1065	JJ	denotes	primary
T14387	1066-1069	NNP	denotes	CD8
T14388	1069-1070	CD	denotes	+
T14389	1071-1072	NNP	denotes	T
T14390	1073-1078	NNS	denotes	cells
T14391	1078-1079	.	denotes	.
T14392	1080-1083	CD	denotes	CD8
T14393	1083-1084	CD	denotes	+
T14394	1085-1086	NNP	denotes	T
T14395	1087-1092	NNS	denotes	cells
T14396	1093-1097	IN	denotes	from
T14397	1098-1099	CD	denotes	4
T14398	1100-1108	JJ	denotes	8-wk-old
T14399	1109-1113	NNP	denotes	Tcra
T14400	1113-1114	FW	denotes	−
T14401	1114-1115	FW	denotes	/
T14402	1115-1116	FW	denotes	−
T14403	1117-1118	FW	denotes	×
T14404	1119-1122	FW	denotes	P14
T14405	1123-1126	FW	denotes	TCR
T14406	1127-1137	FW	denotes	transgenic
T14407	1138-1139	-LRB-	denotes	(
T14408	1139-1146	NNP	denotes	Taconic
T14409	1146-1147	-RRB-	denotes	)
T14410	1147-1148	,	denotes	,
T14411	1149-1157	NNP	denotes	C57BL/6J
T14412	1158-1160	NNP	denotes	WT
T14413	1160-1161	,	denotes	,
T14414	1162-1164	CC	denotes	or
T14415	1165-1170	CD	denotes	Tbx21
T14416	1170-1171	CD	denotes	−
T14417	1171-1172	NN	denotes	/
T14418	1172-1173	NN	denotes	−
T14419	1174-1175	-LRB-	denotes	(
T14420	1175-1178	DT	denotes	The
T14421	1179-1186	NNP	denotes	Jackson
T14422	1187-1197	NNP	denotes	Laboratory
T14423	1197-1198	-RRB-	denotes	)
T14424	1199-1203	NNS	denotes	mice
T14425	1204-1208	VBD	denotes	were
T14426	1209-1217	VBN	denotes	purified
T14427	1218-1219	-LRB-	denotes	(
T14428	1219-1220	FW	denotes	>
T14429	1220-1222	CD	denotes	95
T14430	1222-1223	NN	denotes	%
T14431	1224-1230	NN	denotes	purity
T14432	1230-1231	-RRB-	denotes	)
T14433	1232-1234	IN	denotes	by
T14434	1235-1243	JJ	denotes	negative
T14435	1244-1253	NN	denotes	selection
T14436	1254-1255	-LRB-	denotes	(
T14437	1255-1265	NNP	denotes	Invitrogen
T14438	1265-1266	-RRB-	denotes	)
T14439	1267-1271	IN	denotes	from
T14440	1272-1278	VBN	denotes	pooled
T14441	1279-1285	NN	denotes	spleen
T14442	1286-1289	CC	denotes	and
T14443	1290-1295	NN	denotes	lymph
T14444	1296-1300	NN	denotes	node
T14445	1301-1306	NNS	denotes	cells
T14446	1306-1307	.	denotes	.
T14447	1308-1311	CD	denotes	CD8
T14448	1311-1312	CD	denotes	+
T14449	1313-1314	NNP	denotes	T
T14450	1315-1320	NNS	denotes	cells
T14451	1321-1325	IN	denotes	from
T14452	1326-1331	JJ	denotes	Runx3
T14453	1331-1332	NN	denotes	−
T14454	1332-1333	NN	denotes	/
T14455	1333-1334	NN	denotes	−
T14456	1335-1339	NNS	denotes	mice
T14457	1340-1342	IN	denotes	on
T14458	1343-1346	DT	denotes	the
T14459	1347-1350	NNP	denotes	ICR
T14460	1351-1361	NN	denotes	background
T14461	1362-1366	VBD	denotes	were
T14462	1367-1375	VBN	denotes	purified
T14463	1376-1378	IN	denotes	by
T14464	1379-1387	JJ	denotes	positive
T14465	1388-1397	NN	denotes	selection
T14466	1398-1399	-LRB-	denotes	(
T14467	1399-1407	NNP	denotes	Miltenyi
T14468	1408-1414	NNP	denotes	Biotec
T14469	1414-1415	-RRB-	denotes	)
T14470	1415-1416	.	denotes	.
T14471	1417-1420	DT	denotes	All
T14472	1421-1425	NNS	denotes	mice
T14473	1426-1430	VBD	denotes	were
T14474	1431-1441	VBN	denotes	maintained
T14475	1442-1444	IN	denotes	in
T14476	1445-1453	JJ	denotes	specific
T14477	1454-1467	JJ	denotes	pathogen-free
T14478	1468-1475	NN	denotes	barrier
T14479	1476-1486	NNS	denotes	facilities
T14480	1487-1490	CC	denotes	and
T14481	1491-1495	VBN	denotes	used
T14482	1496-1505	VBG	denotes	according
T14483	1506-1508	TO	denotes	to
T14484	1509-1518	NNS	denotes	protocols
T14485	1519-1527	VBN	denotes	approved
T14486	1528-1530	IN	denotes	by
T14487	1531-1534	DT	denotes	the
T14488	1535-1541	NNP	denotes	Immune
T14489	1542-1549	NNP	denotes	Disease
T14490	1550-1559	NNP	denotes	Institute
T14491	1560-1563	CC	denotes	and
T14492	1564-1567	DT	denotes	the
T14493	1568-1575	NNP	denotes	Harvard
T14494	1576-1583	NNP	denotes	Medical
T14495	1584-1590	NNP	denotes	School
T14496	1591-1597	NNP	denotes	Animal
T14497	1598-1602	NNP	denotes	Care
T14498	1603-1606	CC	denotes	and
T14499	1607-1610	NNP	denotes	Use
T14500	1611-1621	NNS	denotes	Committees
T14501	1621-1622	.	denotes	.
T14502	1623-1626	IN	denotes	For
T14503	1627-1638	NN	denotes	stimulation
T14504	1638-1639	,	denotes	,
T14505	1640-1648	VBN	denotes	purified
T14506	1649-1652	NNP	denotes	CD8
T14507	1652-1653	CD	denotes	+
T14508	1654-1655	NNP	denotes	T
T14509	1656-1661	NNS	denotes	cells
T14510	1662-1666	VBD	denotes	were
T14511	1667-1675	VBN	denotes	cultured
T14512	1676-1678	IN	denotes	at
T14513	1679-1682	CD	denotes	106
T14514	1683-1691	NN	denotes	cells/ml
T14515	1692-1693	-LRB-	denotes	(
T14516	1693-1695	CD	denotes	10
T14517	1696-1698	NN	denotes	ml
T14518	1698-1699	-RRB-	denotes	)
T14519	1700-1702	IN	denotes	in
T14520	1703-1706	CD	denotes	T25
T14521	1707-1713	NNS	denotes	flasks
T14522	1714-1720	VBN	denotes	coated
T14523	1721-1725	IN	denotes	with
T14524	1726-1727	CD	denotes	1
T14525	1728-1730	NN	denotes	μg
T14526	1730-1731	NN	denotes	/
T14527	1731-1733	NN	denotes	ml
T14528	1734-1738	DT	denotes	each
T14529	1739-1741	IN	denotes	of
T14530	1742-1750	JJ	denotes	anti-CD3
T14531	1751-1752	-LRB-	denotes	(
T14532	1752-1757	JJ	denotes	clone
T14533	1758-1762	NNP	denotes	2C11
T14534	1762-1763	-RRB-	denotes	)
T14535	1764-1767	CC	denotes	and
T14536	1768-1777	JJ	denotes	anti-CD28
T14537	1778-1779	-LRB-	denotes	(
T14538	1779-1784	JJ	denotes	clone
T14539	1785-1790	CD	denotes	37.51
T14540	1790-1791	-RRB-	denotes	)
T14541	1792-1794	IN	denotes	by
T14542	1795-1807	NN	denotes	pretreatment
T14543	1808-1812	IN	denotes	with
T14544	1813-1816	CD	denotes	300
T14545	1817-1819	NN	denotes	μg
T14546	1819-1820	NN	denotes	/
T14547	1820-1822	NN	denotes	ml
T14548	1823-1827	NN	denotes	goat
T14549	1828-1832	NNS	denotes	anti
T14550	1833-1840	NN	denotes	hamster
T14551	1841-1844	NNP	denotes	IgG
T14552	1844-1845	.	denotes	.
T14553	1846-1851	IN	denotes	After
T14554	1852-1854	CD	denotes	48
T14555	1855-1856	NN	denotes	h
T14556	1856-1857	,	denotes	,
T14557	1858-1863	NNS	denotes	cells
T14558	1864-1868	VBD	denotes	were
T14559	1869-1876	VBN	denotes	removed
T14560	1877-1881	IN	denotes	from
T14561	1882-1885	DT	denotes	the
T14562	1886-1889	NNP	denotes	TCR
T14563	1890-1901	NN	denotes	stimulation
T14564	1902-1905	CC	denotes	and
T14565	1906-1916	VBD	denotes	recultured
T14566	1917-1919	IN	denotes	at
T14567	1920-1921	DT	denotes	a
T14568	1922-1935	NN	denotes	concentration
T14569	1936-1938	IN	denotes	of
T14570	1939-1940	CD	denotes	5
T14571	1941-1942	CD	denotes	×
T14572	1943-1946	CD	denotes	105
T14573	1947-1955	NN	denotes	cells/ml
T14574	1956-1958	IN	denotes	in
T14575	1959-1964	NNS	denotes	media
T14576	1965-1977	VBN	denotes	supplemented
T14577	1978-1982	IN	denotes	with
T14578	1983-1986	CD	denotes	100
T14579	1987-1991	NNP	denotes	U/ml
T14580	1992-1998	NNP	denotes	rhIL-2
T14581	1998-1999	.	denotes	.
T14582	2000-2005	DT	denotes	Every
T14583	2006-2008	CD	denotes	24
T14584	2009-2010	NN	denotes	h
T14585	2010-2011	,	denotes	,
T14586	2012-2018	JJ	denotes	viable
T14587	2019-2024	NNS	denotes	cells
T14588	2025-2029	VBD	denotes	were
T14589	2030-2037	VBN	denotes	counted
T14590	2038-2041	CC	denotes	and
T14591	2042-2052	VBN	denotes	readjusted
T14592	2053-2055	TO	denotes	to
T14593	2056-2057	CD	denotes	5
T14594	2058-2059	CD	denotes	×
T14595	2060-2063	CD	denotes	105
T14596	2064-2072	NN	denotes	cells/ml
T14597	2073-2077	IN	denotes	with
T14598	2078-2083	JJ	denotes	fresh
T14599	2084-2089	NNS	denotes	media
T14600	2090-2100	VBG	denotes	containing
T14601	2101-2104	DT	denotes	the
T14602	2105-2118	JJ	denotes	corresponding
T14603	2119-2125	NN	denotes	amount
T14604	2126-2128	IN	denotes	of
T14605	2129-2135	NN	denotes	rhIL-2
T14606	2135-2136	.	denotes	.
T15057	2138-2147	NN	denotes	Isolation
T15058	2148-2150	IN	denotes	of
T15059	2151-2154	CD	denotes	CD8
T15060	2154-2155	CD	denotes	+
T15061	2156-2157	NNP	denotes	T
T15062	2158-2163	NNS	denotes	cells
T15063	2164-2168	IN	denotes	from
T15064	2169-2174	JJ	denotes	Runx3
T15065	2174-2175	NN	denotes	−
T15066	2175-2176	NN	denotes	/
T15067	2176-2177	NN	denotes	−
T15068	2178-2182	NNS	denotes	mice
T15069	2182-2183	.	denotes	.
T15070	2184-2199	JJ	denotes	Runx3-deficient
T15071	2200-2201	NNP	denotes	T
T15072	2202-2207	NNS	denotes	cells
T15073	2208-2212	VBP	denotes	fail
T15074	2213-2215	TO	denotes	to
T15075	2216-2223	NN	denotes	silence
T15076	2224-2227	NNP	denotes	CD4
T15077	2228-2238	NN	denotes	expression
T15078	2239-2247	RB	denotes	normally
T15079	2248-2249	-LRB-	denotes	(
T15080	2249-2252	NNP	denotes	Fig
T15081	2252-2253	.	denotes	.
T15082	2254-2256	CD	denotes	S1
T15083	2256-2257	-RRB-	denotes	)
T15084	2258-2259	-LRB-	denotes	(
T15085	2259-2261	CD	denotes	12
T15086	2261-2262	,	denotes	,
T15087	2263-2265	CD	denotes	13
T15088	2265-2266	-RRB-	denotes	)
T15089	2266-2267	.	denotes	.
T15090	2268-2270	PRP	denotes	We
T15091	2271-2280	RB	denotes	therefore
T15092	2281-2288	JJ	denotes	further
T15093	2289-2301	VBD	denotes	fractionated
T15094	2302-2305	DT	denotes	the
T15095	2306-2316	RB	denotes	positively
T15096	2317-2325	VBN	denotes	selected
T15097	2326-2329	NNP	denotes	CD8
T15098	2329-2330	CD	denotes	+
T15099	2331-2332	NNP	denotes	T
T15100	2333-2338	NNS	denotes	cells
T15101	2339-2343	IN	denotes	from
T15102	2344-2349	JJ	denotes	Runx3
T15103	2350-2352	NNP	denotes	KO
T15104	2353-2357	NNS	denotes	mice
T15105	2358-2362	IN	denotes	into
T15106	2363-2366	CD	denotes	CD8
T15107	2366-2367	CD	denotes	+
T15108	2367-2370	NNP	denotes	CD4
T15109	2370-2371	NNP	denotes	−
T15110	2372-2374	NNP	denotes	SP
T15111	2375-2377	CC	denotes	or
T15112	2378-2381	NNP	denotes	CD8
T15113	2381-2382	NNP	denotes	+
T15114	2382-2385	NNP	denotes	CD4
T15115	2385-2386	NNP	denotes	+
T15116	2387-2389	NNP	denotes	DP
T15117	2390-2395	NNS	denotes	cells
T15118	2396-2398	IN	denotes	by
T15119	2399-2409	NN	denotes	separation
T15120	2410-2415	VBG	denotes	using
T15121	2416-2424	JJ	denotes	anti-CD4
T15122	2425-2433	JJ	denotes	magnetic
T15123	2434-2439	NNS	denotes	beads
T15124	2439-2440	.	denotes	.
T15125	2441-2445	DT	denotes	This
T15126	2446-2453	VBD	denotes	yielded
T15127	2454-2455	DT	denotes	a
T15128	2456-2461	JJ	denotes	Runx3
T15129	2462-2464	NNP	denotes	KO
T15130	2465-2467	NNP	denotes	SP
T15131	2469-2477	VBD	denotes	enriched
T15132	2479-2489	NN	denotes	population
T15133	2490-2494	WDT	denotes	that
T15134	2495-2504	VBD	denotes	contained
T15135	2505-2507	CD	denotes	75
T15136	2507-2508	NN	denotes	%
T15137	2509-2512	CD	denotes	CD8
T15138	2512-2513	NN	denotes	+
T15139	2513-2516	CD	denotes	CD4
T15140	2516-2517	NN	denotes	−
T15141	2518-2523	NNS	denotes	cells
T15142	2524-2527	CC	denotes	and
T15143	2528-2529	DT	denotes	a
T15144	2530-2532	NNP	denotes	KO
T15145	2533-2535	NNP	denotes	DP
T15146	2536-2544	VBD	denotes	enriched
T15147	2545-2555	NN	denotes	population
T15148	2556-2560	WDT	denotes	that
T15149	2561-2570	VBD	denotes	contained
T15150	2571-2573	CD	denotes	85
T15151	2573-2574	NN	denotes	%
T15152	2575-2578	CD	denotes	CD8
T15153	2578-2579	NN	denotes	+
T15154	2579-2582	CD	denotes	CD4
T15155	2582-2583	NN	denotes	+
T15156	2584-2589	NNS	denotes	cells
T15157	2590-2591	-LRB-	denotes	(
T15158	2591-2594	NNP	denotes	Fig
T15159	2594-2595	.	denotes	.
T15160	2596-2598	CD	denotes	S1
T15161	2598-2599	-RRB-	denotes	)
T15162	2599-2600	.	denotes	.
T15163	2601-2604	DT	denotes	The
T15164	2605-2610	NNS	denotes	cells
T15165	2611-2615	VBD	denotes	were
T15166	2616-2626	VBN	denotes	stimulated
T15167	2627-2631	IN	denotes	with
T15168	2632-2640	JJ	denotes	anti-CD3
T15169	2640-2641	NN	denotes	+
T15170	2642-2651	NN	denotes	anti-CD28
T15171	2652-2655	IN	denotes	for
T15172	2656-2657	CD	denotes	2
T15173	2658-2659	LS	denotes	d
T15174	2660-2666	IN	denotes	before
T15175	2667-2675	VBG	denotes	removing
T15176	2676-2680	PRP	denotes	them
T15177	2681-2685	IN	denotes	from
T15178	2686-2689	DT	denotes	the
T15179	2690-2693	NNP	denotes	TCR
T15180	2694-2702	NN	denotes	stimulus
T15181	2703-2706	CC	denotes	and
T15182	2707-2716	VBG	denotes	culturing
T15183	2717-2721	PRP	denotes	them
T15184	2722-2724	IN	denotes	in
T15185	2725-2730	NNS	denotes	media
T15186	2731-2741	VBG	denotes	containing
T15187	2742-2745	CD	denotes	100
T15188	2746-2750	NNP	denotes	U/ml
T15189	2751-2755	NNP	denotes	IL-2
T15190	2755-2756	.	denotes	.
T15191	2757-2759	IN	denotes	As
T15192	2760-2770	RB	denotes	previously
T15193	2771-2779	VBN	denotes	reported
T15194	2779-2780	,	denotes	,
T15195	2781-2792	JJ	denotes	TCR-induced
T15196	2793-2806	NN	denotes	proliferation
T15197	2807-2809	IN	denotes	of
T15198	2810-2815	NNP	denotes	Runx3
T15199	2815-2816	FW	denotes	−
T15200	2816-2817	FW	denotes	/
T15201	2817-2818	FW	denotes	−
T15202	2819-2822	FW	denotes	CD8
T15203	2822-2823	FW	denotes	+
T15204	2824-2825	FW	denotes	T
T15205	2826-2831	NNS	denotes	cells
T15206	2832-2835	VBD	denotes	was
T15207	2836-2844	RB	denotes	severely
T15208	2845-2853	VBN	denotes	impaired
T15209	2853-2854	,	denotes	,
T15210	2855-2867	RB	denotes	irrespective
T15211	2868-2870	IN	denotes	of
T15212	2871-2874	CD	denotes	CD4
T15213	2875-2885	NN	denotes	expression
T15214	2886-2887	-LRB-	denotes	(
T15215	2887-2890	NNP	denotes	Fig
T15216	2890-2891	.	denotes	.
T15217	2892-2894	CD	denotes	S1
T15218	2894-2895	-RRB-	denotes	)
T15219	2896-2897	-LRB-	denotes	(
T15220	2897-2899	CD	denotes	12
T15221	2899-2900	,	denotes	,
T15222	2901-2903	CD	denotes	13
T15223	2903-2904	-RRB-	denotes	)
T15224	2904-2905	.	denotes	.
T15225	2906-2913	RB	denotes	However
T15226	2913-2914	,	denotes	,
T15227	2915-2918	DT	denotes	the
T15228	2919-2924	NNP	denotes	Runx3
T15229	2924-2925	NNP	denotes	−
T15230	2925-2926	VBD	denotes	/
T15231	2926-2927	CD	denotes	−
T15232	2928-2933	NNS	denotes	cells
T15233	2934-2940	VBD	denotes	showed
T15234	2941-2953	JJ	denotes	cell-surface
T15235	2954-2964	NN	denotes	expression
T15236	2965-2973	NNS	denotes	patterns
T15237	2974-2984	JJ	denotes	indicative
T15238	2985-2987	IN	denotes	of
T15239	2988-2997	VBN	denotes	activated
T15240	2998-3003	NNS	denotes	cells
T15241	3003-3004	,	denotes	,
T15242	3005-3014	VBG	denotes	including
T15243	3015-3028	NN	denotes	up-regulation
T15244	3029-3031	IN	denotes	of
T15245	3032-3036	NNP	denotes	CD25
T15246	3037-3040	CC	denotes	and
T15247	3041-3045	NNP	denotes	CD69
T15248	3046-3047	-LRB-	denotes	(
T15249	3047-3050	NNP	denotes	Fig
T15250	3050-3051	.	denotes	.
T15251	3052-3054	CD	denotes	S1
T15252	3054-3055	-RRB-	denotes	)
T15253	3055-3056	.	denotes	.
T15254	3057-3059	IN	denotes	As
T15255	3060-3068	VBN	denotes	expected
T15256	3069-3073	IN	denotes	from
T15257	3074-3079	PRP$	denotes	their
T15258	3080-3087	NN	denotes	ability
T15259	3088-3090	TO	denotes	to
T15260	3091-3102	VB	denotes	up-regulate
T15261	3103-3107	NNP	denotes	CD25
T15262	3107-3108	,	denotes	,
T15263	3109-3114	NNP	denotes	Runx3
T15264	3114-3115	FW	denotes	−
T15265	3115-3116	FW	denotes	/
T15266	3116-3117	FW	denotes	−
T15267	3118-3121	FW	denotes	CD8
T15268	3121-3122	FW	denotes	+
T15269	3123-3124	FW	denotes	T
T15270	3125-3130	NNS	denotes	cells
T15271	3131-3140	VBD	denotes	responded
T15272	3141-3143	TO	denotes	to
T15273	3144-3148	NNP	denotes	IL-2
T15274	3149-3164	NN	denotes	supplementation
T15275	3165-3170	IN	denotes	after
T15276	3171-3174	NN	denotes	day
T15277	3175-3176	CD	denotes	2
T15278	3177-3180	CC	denotes	and
T15279	3181-3192	RB	denotes	efficiently
T15280	3193-3201	VBN	denotes	expanded
T15281	3202-3207	IN	denotes	until
T15282	3208-3211	NN	denotes	day
T15283	3212-3213	CD	denotes	6
T15284	3214-3216	IN	denotes	of
T15285	3217-3220	DT	denotes	the
T15286	3221-3228	NN	denotes	culture
T15287	3229-3235	NN	denotes	period
T15288	3235-3236	,	denotes	,
T15289	3237-3243	IN	denotes	albeit
T15290	3244-3246	IN	denotes	at
T15291	3247-3253	JJR	denotes	slower
T15292	3254-3259	NNS	denotes	rates
T15293	3260-3268	VBN	denotes	compared
T15294	3269-3273	IN	denotes	with
T15295	3274-3276	NNP	denotes	WT
T15296	3277-3282	NNS	denotes	cells
T15297	3283-3284	-LRB-	denotes	(
T15298	3284-3287	NNP	denotes	Fig
T15299	3287-3288	.	denotes	.
T15300	3289-3291	CD	denotes	S1
T15301	3291-3292	-RRB-	denotes	)
T15302	3292-3293	.	denotes	.
T15303	3294-3302	IN	denotes	Although
T15304	3303-3304	DT	denotes	a
T15305	3305-3313	NN	denotes	fraction
T15306	3314-3316	IN	denotes	of
T15307	3317-3320	DT	denotes	the
T15308	3321-3323	NNP	denotes	KO
T15309	3324-3326	NNP	denotes	DP
T15310	3327-3332	NNS	denotes	cells
T15311	3333-3341	VBD	denotes	silenced
T15312	3342-3345	CD	denotes	CD4
T15313	3346-3356	NN	denotes	expression
T15314	3357-3362	IN	denotes	after
T15315	3363-3373	NN	denotes	activation
T15316	3373-3374	,	denotes	,
T15317	3375-3378	DT	denotes	the
T15318	3379-3384	NN	denotes	ratio
T15319	3385-3387	IN	denotes	of
T15320	3388-3393	NNP	denotes	SP/DP
T15321	3394-3399	NNS	denotes	cells
T15322	3400-3402	IN	denotes	in
T15323	3403-3407	DT	denotes	each
T15324	3408-3416	VBN	denotes	enriched
T15325	3417-3427	NN	denotes	population
T15326	3428-3436	VBD	denotes	remained
T15327	3437-3445	JJ	denotes	constant
T15328	3446-3456	RB	denotes	thereafter
T15329	3456-3457	,	denotes	,
T15330	3458-3461	CC	denotes	and
T15331	3462-3464	PRP	denotes	we
T15332	3465-3468	VBD	denotes	did
T15333	3469-3472	RB	denotes	not
T15334	3473-3480	VB	denotes	observe
T15335	3481-3484	DT	denotes	any
T15336	3485-3490	JJ	denotes	major
T15337	3491-3502	NNS	denotes	differences
T15338	3503-3510	IN	denotes	between
T15339	3511-3516	DT	denotes	these
T15340	3517-3520	CD	denotes	two
T15341	3521-3532	NNS	denotes	populations
T15342	3533-3543	IN	denotes	throughout
T15343	3544-3547	DT	denotes	the
T15344	3548-3555	NN	denotes	culture
T15345	3556-3562	NN	denotes	period
T15346	3562-3563	,	denotes	,
T15347	3564-3574	VBG	denotes	indicating
T15348	3575-3579	IN	denotes	that
T15349	3580-3582	IN	denotes	in
T15350	3583-3588	NNS	denotes	terms
T15351	3589-3591	IN	denotes	of
T15352	3592-3600	NN	denotes	effector
T15353	3601-3604	NNP	denotes	CTL
T15354	3605-3620	NN	denotes	differentiation
T15355	3621-3624	CC	denotes	and
T15356	3625-3630	IN	denotes	under
T15357	3631-3634	PRP$	denotes	our
T15358	3635-3642	NN	denotes	culture
T15359	3643-3653	NNS	denotes	conditions
T15360	3653-3654	,	denotes	,
T15361	3655-3660	JJ	denotes	Runx3
T15362	3660-3661	FW	denotes	−
T15363	3661-3662	FW	denotes	/
T15364	3662-3663	FW	denotes	−
T15365	3664-3667	FW	denotes	CD8
T15366	3667-3668	FW	denotes	+
T15367	3669-3670	FW	denotes	T
T15368	3671-3676	NNS	denotes	cells
T15369	3677-3681	WDT	denotes	that
T15370	3682-3686	RB	denotes	also
T15371	3687-3696	VBZ	denotes	coexpress
T15372	3697-3700	CD	denotes	CD4
T15373	3701-3704	VBP	denotes	are
T15374	3705-3722	JJ	denotes	indistinguishable
T15375	3723-3727	IN	denotes	from
T15376	3728-3733	DT	denotes	those
T15377	3734-3738	WDT	denotes	that
T15378	3739-3741	VBP	denotes	do
T15379	3742-3745	RB	denotes	not
T15380	3745-3746	.	denotes	.
T15381	3747-3750	DT	denotes	The
T15382	3751-3755	NNS	denotes	data
T15383	3756-3765	VBN	denotes	presented
T15384	3766-3768	IN	denotes	in
T15385	3769-3772	NNP	denotes	Fig
T15386	3772-3773	.	denotes	.
T15387	3774-3776	CD	denotes	S2
T15388	3777-3780	VBP	denotes	are
T15389	3781-3785	IN	denotes	from
T15390	3786-3791	JJ	denotes	Runx3
T15391	3792-3794	NNP	denotes	KO
T15392	3795-3797	NNP	denotes	SP
T15393	3798-3803	NNS	denotes	cells
T15394	3803-3804	,	denotes	,
T15395	3805-3812	IN	denotes	whereas
T15396	3813-3818	DT	denotes	those
T15397	3819-3824	VBN	denotes	shown
T15398	3825-3827	IN	denotes	in
T15399	3828-3833	NNP	denotes	Figs.
T15400	3834-3835	CD	denotes	3
T15401	3836-3839	CC	denotes	and
T15402	3840-3841	CD	denotes	4
T15403	3842-3845	VBP	denotes	are
T15404	3846-3850	IN	denotes	from
T15405	3851-3856	JJ	denotes	total
T15406	3857-3862	JJ	denotes	Runx3
T15407	3863-3865	NNP	denotes	KO
T15408	3866-3869	NNP	denotes	CD8
T15409	3870-3875	NNS	denotes	cells
T15410	3875-3876	.	denotes	.
T16130	3878-3888	JJ	denotes	FACS-based
T16131	3889-3901	NN	denotes	cytotoxicity
T16132	3902-3907	NN	denotes	assay
T16133	3907-3908	.	denotes	.
T16134	3909-3911	TO	denotes	To
T16135	3912-3919	VB	denotes	measure
T16136	3920-3932	NN	denotes	cytotoxicity
T16137	3932-3933	,	denotes	,
T16138	3934-3937	CD	denotes	EL4
T16139	3938-3945	NN	denotes	thymoma
T16140	3946-3952	NN	denotes	target
T16141	3953-3958	NNS	denotes	cells
T16142	3959-3963	VBD	denotes	were
T16143	3964-3970	VBN	denotes	loaded
T16144	3971-3975	IN	denotes	with
T16145	3976-3977	CD	denotes	0
T16146	3978-3980	CC	denotes	or
T16147	3981-3982	CD	denotes	1
T16148	3983-3985	NNP	denotes	μM
T16149	3986-3990	NNP	denotes	Gp33
T16150	3991-3998	NN	denotes	peptide
T16151	3999-4002	IN	denotes	for
T16152	4003-4004	CD	denotes	2
T16153	4005-4006	NN	denotes	h
T16154	4007-4013	IN	denotes	before
T16155	4014-4015	DT	denotes	a
T16156	4016-4019	JJ	denotes	2-h
T16157	4020-4032	NN	denotes	coincubation
T16158	4033-4037	IN	denotes	with
T16159	4038-4041	NNP	denotes	P14
T16160	4042-4045	NNP	denotes	CD8
T16161	4045-4046	VBD	denotes	+
T16162	4047-4048	NNP	denotes	T
T16163	4049-4054	NNS	denotes	cells
T16164	4055-4057	IN	denotes	at
T16165	4058-4061	DT	denotes	the
T16166	4062-4080	JJ	denotes	effector-to-target
T16167	4081-4087	NNS	denotes	ratios
T16168	4088-4097	VBD	denotes	indicated
T16169	4098-4100	IN	denotes	in
T16170	4101-4104	DT	denotes	the
T16171	4105-4112	NNS	denotes	figures
T16172	4113-4115	IN	denotes	in
T16173	4116-4123	JJ	denotes	96-well
T16174	4124-4136	NN	denotes	round-bottom
T16175	4137-4143	NNS	denotes	plates
T16176	4143-4144	.	denotes	.
T16177	4145-4150	IN	denotes	After
T16178	4151-4154	DT	denotes	the
T16179	4155-4167	NN	denotes	coincubation
T16180	4168-4174	NN	denotes	period
T16181	4174-4175	,	denotes	,
T16182	4176-4181	NNS	denotes	cells
T16183	4182-4186	VBD	denotes	were
T16184	4187-4194	VBN	denotes	stained
T16185	4195-4199	IN	denotes	with
T16186	4200-4207	NNP	denotes	Annexin
T16187	4208-4209	NNP	denotes	V
T16188	4210-4214	NNP	denotes	FITC
T16189	4215-4218	CC	denotes	and
T16190	4219-4227	JJ	denotes	anti-CD8
T16191	4228-4243	NN	denotes	allophycocyanin
T16192	4243-4244	.	denotes	.
T16193	4245-4249	NNP	denotes	Data
T16194	4250-4258	NN	denotes	analysis
T16195	4259-4262	VBD	denotes	was
T16196	4263-4272	VBN	denotes	performed
T16197	4273-4277	IN	denotes	with
T16198	4278-4284	NNP	denotes	FlowJo
T16199	4285-4293	NN	denotes	software
T16200	4294-4295	-LRB-	denotes	(
T16201	4295-4299	NNP	denotes	Tree
T16202	4300-4304	NNP	denotes	Star
T16203	4304-4305	,	denotes	,
T16204	4306-4310	NNP	denotes	Inc.
T16205	4310-4311	-RRB-	denotes	)
T16206	4311-4312	:	denotes	;
T16207	4313-4316	CD	denotes	EL4
T16208	4317-4323	NN	denotes	target
T16209	4324-4329	NNS	denotes	cells
T16210	4330-4331	-LRB-	denotes	(
T16211	4331-4343	JJ	denotes	CD8-negative
T16212	4344-4350	NNS	denotes	events
T16213	4350-4351	-RRB-	denotes	)
T16214	4352-4356	VBD	denotes	were
T16215	4357-4362	VBN	denotes	gated
T16216	4362-4363	,	denotes	,
T16217	4364-4367	CC	denotes	and
T16218	4368-4371	DT	denotes	the
T16219	4372-4382	NN	denotes	percentage
T16220	4383-4385	IN	denotes	of
T16221	4386-4393	NNP	denotes	Annexin
T16222	4394-4395	NNP	denotes	V
T16223	4395-4396	VBD	denotes	+
T16224	4397-4403	NN	denotes	target
T16225	4404-4409	NNS	denotes	cells
T16226	4410-4413	VBD	denotes	was
T16227	4414-4424	VBN	denotes	determined
T16228	4424-4425	.	denotes	.
T16385	4427-4435	NNP	denotes	Cytokine
T16386	4436-4439	CC	denotes	and
T16387	4440-4447	NN	denotes	surface
T16388	4448-4454	NN	denotes	marker
T16389	4455-4463	VBG	denotes	staining
T16390	4463-4464	.	denotes	.
T16391	4465-4467	TO	denotes	To
T16392	4468-4474	VB	denotes	assess
T16393	4475-4483	JJ	denotes	cytokine
T16394	4484-4494	NN	denotes	production
T16395	4494-4495	,	denotes	,
T16396	4496-4501	NNS	denotes	cells
T16397	4502-4506	VBD	denotes	were
T16398	4507-4519	VBN	denotes	restimulated
T16399	4520-4524	IN	denotes	with
T16400	4525-4527	CD	denotes	10
T16401	4528-4530	NNP	denotes	nM
T16402	4531-4534	NNP	denotes	PMA
T16403	4535-4536	VBD	denotes	+
T16404	4537-4538	CD	denotes	1
T16405	4539-4541	NNP	denotes	μM
T16406	4542-4551	NN	denotes	ionomycin
T16407	4552-4555	IN	denotes	for
T16408	4556-4557	CD	denotes	6
T16409	4558-4559	NN	denotes	h
T16410	4560-4561	-LRB-	denotes	(
T16411	4561-4567	IN	denotes	unless
T16412	4568-4577	VBN	denotes	indicated
T16413	4578-4587	RB	denotes	otherwise
T16414	4588-4590	IN	denotes	in
T16415	4591-4594	DT	denotes	the
T16416	4595-4602	NNS	denotes	figures
T16417	4602-4603	-RRB-	denotes	)
T16418	4603-4604	,	denotes	,
T16419	4605-4608	CC	denotes	and
T16420	4609-4622	JJ	denotes	intracellular
T16421	4623-4631	NN	denotes	cytokine
T16422	4632-4638	NNS	denotes	stains
T16423	4639-4643	VBD	denotes	were
T16424	4644-4653	VBN	denotes	performed
T16425	4654-4656	IN	denotes	as
T16426	4657-4667	RB	denotes	previously
T16427	4668-4677	VBN	denotes	described
T16428	4678-4679	-LRB-	denotes	(
T16429	4679-4681	CD	denotes	28
T16430	4681-4682	-RRB-	denotes	)
T16431	4682-4683	.	denotes	.
T16432	4684-4686	TO	denotes	To
T16433	4687-4693	VB	denotes	detect
T16434	4694-4704	NN	denotes	expression
T16435	4705-4707	IN	denotes	of
T16436	4708-4715	NN	denotes	surface
T16437	4716-4725	NNS	denotes	molecules
T16438	4725-4726	,	denotes	,
T16439	4727-4732	NNS	denotes	cells
T16440	4733-4737	VBD	denotes	were
T16441	4738-4744	VBN	denotes	washed
T16442	4745-4747	IN	denotes	in
T16443	4748-4751	NNP	denotes	PBS
T16444	4751-4752	,	denotes	,
T16445	4753-4764	VBD	denotes	resuspended
T16446	4765-4767	IN	denotes	in
T16447	4768-4772	NNP	denotes	FACS
T16448	4773-4777	VBP	denotes	wash
T16449	4778-4784	NN	denotes	buffer
T16450	4785-4786	-LRB-	denotes	(
T16451	4786-4787	CD	denotes	3
T16452	4787-4788	NN	denotes	%
T16453	4789-4792	NNP	denotes	FBS
T16454	4792-4793	,	denotes	,
T16455	4794-4797	CD	denotes	0.1
T16456	4797-4798	NN	denotes	%
T16457	4799-4805	NN	denotes	sodium
T16458	4806-4811	NN	denotes	azide
T16459	4811-4812	,	denotes	,
T16460	4813-4815	CD	denotes	30
T16461	4816-4818	NNP	denotes	mM
T16462	4819-4824	NNP	denotes	Hepes
T16463	4824-4825	,	denotes	,
T16464	4826-4827	CD	denotes	1
T16465	4827-4828	CD	denotes	×
T16466	4829-4832	NNP	denotes	PBS
T16467	4832-4833	-RRB-	denotes	)
T16468	4834-4844	VBG	denotes	containing
T16469	4845-4848	DT	denotes	the
T16470	4849-4859	NNS	denotes	antibodies
T16471	4860-4869	VBD	denotes	indicated
T16472	4870-4872	IN	denotes	in
T16473	4873-4876	DT	denotes	the
T16474	4877-4884	NNS	denotes	figures
T16475	4885-4887	IN	denotes	at
T16476	4888-4898	RB	denotes	previously
T16477	4899-4908	VBN	denotes	optimized
T16478	4909-4923	NNS	denotes	concentrations
T16479	4923-4924	,	denotes	,
T16480	4925-4934	VBN	denotes	incubated
T16481	4935-4938	IN	denotes	for
T16482	4939-4941	CD	denotes	15
T16483	4942-4945	NN	denotes	min
T16484	4946-4948	IN	denotes	at
T16485	4949-4953	NN	denotes	room
T16486	4954-4965	NN	denotes	temperature
T16487	4966-4967	-LRB-	denotes	(
T16488	4967-4969	NNP	denotes	RT
T16489	4969-4970	-RRB-	denotes	)
T16490	4970-4971	,	denotes	,
T16491	4972-4978	VBD	denotes	washed
T16492	4978-4979	,	denotes	,
T16493	4980-4983	CC	denotes	and
T16494	4984-4995	VBD	denotes	resuspended
T16495	4996-4998	IN	denotes	in
T16496	4999-5000	CD	denotes	2
T16497	5000-5001	NN	denotes	%
T16498	5002-5014	NN	denotes	formaldehyde
T16499	5015-5023	JJ	denotes	fixative
T16500	5024-5032	NN	denotes	solution
T16501	5033-5039	IN	denotes	before
T16502	5040-5051	NN	denotes	acquisition
T16503	5052-5054	IN	denotes	on
T16504	5055-5056	DT	denotes	a
T16505	5057-5068	NNP	denotes	FACSCalibur
T16506	5069-5070	-LRB-	denotes	(
T16507	5070-5072	NNP	denotes	BD
T16508	5072-5073	-RRB-	denotes	)
T16509	5073-5074	.	denotes	.
T16656	5076-5086	NNP	denotes	Retroviral
T16657	5087-5099	NN	denotes	transduction
T16658	5100-5102	IN	denotes	of
T16659	5103-5110	JJ	denotes	primary
T16660	5111-5114	NNP	denotes	CD8
T16661	5114-5115	CD	denotes	+
T16662	5116-5117	NNP	denotes	T
T16663	5118-5123	NNS	denotes	cells
T16664	5123-5124	.	denotes	.
T16665	5125-5128	IN	denotes	For
T16666	5129-5141	NN	denotes	transduction
T16667	5142-5153	NNS	denotes	experiments
T16668	5153-5154	,	denotes	,
T16669	5155-5160	JJ	denotes	viral
T16670	5161-5173	NNS	denotes	supernatants
T16671	5174-5178	VBD	denotes	were
T16672	5179-5188	VBN	denotes	generated
T16673	5189-5191	IN	denotes	by
T16674	5192-5199	NN	denotes	calcium
T16675	5200-5209	NN	denotes	phosphate
T16676	5210-5222	NN	denotes	transfection
T16677	5223-5225	IN	denotes	of
T16678	5226-5233	NNP	denotes	Phoenix
T16679	5234-5239	NNS	denotes	cells
T16680	5240-5243	CC	denotes	and
T16681	5244-5257	NN	denotes	concentration
T16682	5258-5260	IN	denotes	by
T16683	5261-5270	JJ	denotes	overnight
T16684	5271-5285	NN	denotes	centrifugation
T16685	5286-5288	IN	denotes	at
T16686	5289-5294	CD	denotes	6,000
T16687	5295-5296	NN	denotes	g
T16688	5296-5297	.	denotes	.
T16689	5298-5300	IN	denotes	At
T16690	5301-5302	CD	denotes	∼
T16691	5302-5304	CD	denotes	42
T16692	5305-5306	NN	denotes	h
T16693	5307-5312	IN	denotes	after
T16694	5313-5316	DT	denotes	the
T16695	5317-5324	JJ	denotes	initial
T16696	5325-5328	NNP	denotes	TCR
T16697	5329-5339	NN	denotes	activation
T16698	5340-5342	IN	denotes	of
T16699	5343-5346	CD	denotes	106
T16700	5347-5350	CD	denotes	CD8
T16701	5350-5351	NN	denotes	+
T16702	5352-5353	NNP	denotes	T
T16703	5354-5359	NNS	denotes	cells
T16721	5464-5468	IN	denotes	with
T16722	5469-5470	CD	denotes	8
T16723	5471-5473	NN	denotes	μg
T16724	5473-5474	NN	denotes	/
T16725	5474-5476	NN	denotes	ml
T16726	5477-5486	NN	denotes	polybrene
T16727	5487-5497	VBG	denotes	containing
T16728	5498-5503	JJ	denotes	fresh
T16729	5504-5508	CC	denotes	plus
T16730	5509-5521	JJ	denotes	concentrated
T16731	5522-5527	NN	denotes	virus
T16732	5527-5528	.	denotes	.
T16733	5529-5532	DT	denotes	The
T16734	5533-5539	NNS	denotes	plates
T16735	5540-5544	VBD	denotes	were
T16736	5545-5556	VBN	denotes	centrifuged
T16737	5557-5559	IN	denotes	at
T16738	5560-5563	CD	denotes	700
T16739	5564-5565	NN	denotes	g
T16740	5566-5569	IN	denotes	for
T16741	5570-5571	CD	denotes	1
T16742	5572-5573	NN	denotes	h
T16743	5574-5576	IN	denotes	at
T16744	5577-5579	NNP	denotes	RT
T16745	5580-5586	IN	denotes	before
T16746	5587-5596	VBG	denotes	returning
T16747	5597-5599	TO	denotes	to
T16748	5600-5602	CD	denotes	37
T16749	5602-5603	CD	denotes	°
T16750	5603-5604	NNP	denotes	C
T16751	5605-5608	IN	denotes	for
T16752	5609-5611	DT	denotes	an
T16753	5612-5622	JJ	denotes	additional
T16754	5623-5624	CD	denotes	5
T16755	5625-5627	NN	denotes	h.
T16756	5628-5638	NNP	denotes	Retroviral
T16757	5639-5649	NNS	denotes	constructs
T16758	5650-5653	IN	denotes	for
T16759	5654-5664	NNP	denotes	Eomes-VP16
T16760	5665-5668	CC	denotes	and
T16761	5669-5672	DT	denotes	the
T16762	5673-5676	NNP	denotes	MIG
T16763	5677-5684	NN	denotes	control
T16764	5685-5690	JJ	denotes	empty
T16765	5691-5697	NN	denotes	vector
T16766	5698-5702	VBD	denotes	were
T16767	5703-5704	DT	denotes	a
T16768	5705-5709	NN	denotes	gift
T16769	5710-5714	IN	denotes	from
T16770	5715-5719	NNP	denotes	S.L.
T16771	5720-5726	NNP	denotes	Reiner
T16772	5727-5728	-LRB-	denotes	(
T16773	5728-5738	NNP	denotes	University
T16774	5739-5741	IN	denotes	of
T16775	5742-5754	NNP	denotes	Pennsylvania
T16776	5754-5755	,	denotes	,
T16777	5756-5768	NNP	denotes	Philadelphia
T16778	5768-5769	,	denotes	,
T16779	5770-5772	NNP	denotes	PA
T16780	5772-5773	-RRB-	denotes	)
T16781	5774-5775	-LRB-	denotes	(
T16782	5775-5776	CD	denotes	8
T16783	5776-5777	-RRB-	denotes	)
T16784	5777-5778	.	denotes	.
T17610	8544-8549	NN	denotes	nylon
T17611	8550-8559	NNS	denotes	membranes
T17612	8560-8561	-LRB-	denotes	(
T17613	8561-8569	JJ	denotes	Hybond-N
T17614	8569-8570	NN	denotes	+
T17615	8570-8571	:	denotes	;
T17616	8572-8574	NNP	denotes	GE
T17617	8575-8585	NNP	denotes	Healthcare
T17618	8585-8586	-RRB-	denotes	)
T17619	8586-8587	,	denotes	,
T17620	8588-8593	WDT	denotes	which
T17621	8594-8597	VBD	denotes	was
T17622	8598-8607	VBN	denotes	confirmed
T17623	8608-8610	IN	denotes	by
T17624	8611-8619	NN	denotes	ethidium
T17625	8620-8627	NN	denotes	bromide
T17626	8628-8636	NN	denotes	staining
T17627	8637-8639	IN	denotes	of
T17628	8640-8649	JJ	denotes	ribosomal
T17629	8650-8653	NNP	denotes	RNA
T17630	8654-8661	NNS	denotes	species
T17631	8662-8664	IN	denotes	on
T17632	8665-8668	DT	denotes	the
T17633	8669-8677	NN	denotes	membrane
T17634	8677-8678	.	denotes	.
T17635	8679-8688	NNS	denotes	Membranes
T17636	8689-8693	VBD	denotes	were
T17637	8694-8704	VBN	denotes	hybridized
T17638	8705-8709	IN	denotes	with
T17639	8710-8711	CD	denotes	1
T17640	8712-8717	NN	denotes	ng/ml
T17641	8718-8719	SYM	denotes	α
T17642	8719-8720	:	denotes	-
T17643	8720-8721	NNP	denotes	[
T17644	8721-8724	NNP	denotes	32P
T17645	8724-8725	NNP	denotes	]
T17646	8725-8729	NNP	denotes	dCTP
T17647	8730-8737	VBN	denotes	labeled
T17648	8738-8753	JJ	denotes	trichloroacetic
T17649	8754-8758	NN	denotes	acid
T17650	8759-8771	JJ	denotes	precipitable
T17651	8772-8777	NN	denotes	probe
T17652	8778-8780	IN	denotes	in
T17653	8781-8791	NNP	denotes	ExpressHyb
T17654	8792-8805	NN	denotes	hybridization
T17655	8806-8812	NN	denotes	buffer
T17656	8813-8814	-LRB-	denotes	(
T17657	8814-8822	NNP	denotes	Clontech
T17658	8823-8835	NNPS	denotes	Laboratories
T17659	8835-8836	,	denotes	,
T17660	8837-8841	NNP	denotes	Inc.
T17661	8841-8842	-RRB-	denotes	)
T17662	8842-8843	.	denotes	.
T17663	8844-8847	DT	denotes	All
T17664	8848-8852	NNP	denotes	cDNA
T17665	8853-8859	NNS	denotes	probes
T17666	8860-8864	VBD	denotes	were
T17667	8865-8874	VBN	denotes	confirmed
T17668	8875-8877	TO	denotes	to
T17669	8878-8882	VB	denotes	have
T17670	8883-8886	DT	denotes	the
T17671	8887-8898	JJ	denotes	appropriate
T17672	8899-8910	JJ	denotes	single-copy
T17673	8911-8922	NN	denotes	specificity
T17674	8923-8928	IN	denotes	under
T17675	8929-8934	DT	denotes	these
T17676	8935-8945	NNS	denotes	conditions
T17677	8946-8951	VBG	denotes	using
T17678	8952-8959	JJ	denotes	genomic
T17679	8960-8968	NNP	denotes	Southern
T17680	8969-8973	NN	denotes	blot
T17681	8974-8982	NN	denotes	analysis
T17682	8982-8983	.	denotes	.
T17683	8984-8988	NNP	denotes	Band
T17684	8989-9000	NNS	denotes	intensities
T17685	9001-9005	VBD	denotes	were
T17686	9006-9014	VBN	denotes	acquired
T17687	9015-9017	IN	denotes	by
T17688	9018-9033	VBG	denotes	phosphorimaging
T17689	9034-9042	NN	denotes	analysis
T17690	9042-9043	.	denotes	.
T17691	9044-9047	IN	denotes	For
T17692	9048-9055	JJ	denotes	Western
T17693	9056-9064	NN	denotes	analysis
T17694	9064-9065	,	denotes	,
T17695	9066-9076	JJ	denotes	whole-cell
T17696	9077-9084	NN	denotes	protein
T17697	9085-9092	NNS	denotes	lysates
T17698	9093-9097	VBD	denotes	were
T17699	9098-9106	VBN	denotes	obtained
T17700	9107-9111	IN	denotes	from
T17701	9112-9115	CD	denotes	CD8
T17702	9115-9116	CD	denotes	+
T17703	9117-9118	NNP	denotes	T
T17704	9119-9124	NNS	denotes	cells
T17705	9125-9127	IN	denotes	at
T17706	9128-9131	DT	denotes	the
T17707	9132-9136	NN	denotes	time
T17708	9137-9143	NNS	denotes	points
T17709	9144-9153	VBD	denotes	indicated
T17710	9154-9156	IN	denotes	in
T17711	9157-9160	DT	denotes	the
T17712	9161-9168	NNS	denotes	figures
T17713	9169-9175	IN	denotes	during
T17714	9176-9182	JJ	denotes	clonal
T17715	9183-9192	NN	denotes	expansion
T17716	9193-9195	IN	denotes	in
T17717	9196-9199	CD	denotes	100
T17718	9200-9204	NNP	denotes	U/ml
T17719	9205-9209	NN	denotes	IL-2
T17720	9210-9214	IN	denotes	with
T17721	9215-9220	NN	denotes	lysis
T17722	9221-9227	NN	denotes	buffer
T17723	9228-9229	-LRB-	denotes	(
T17724	9229-9231	CD	denotes	50
T17725	9232-9234	NNP	denotes	mM
T17726	9235-9239	NNP	denotes	Tris
T17727	9240-9241	NNP	denotes	[
T17728	9241-9243	NNP	denotes	pH
T17729	9244-9247	CD	denotes	7.5
T17730	9247-9248	NNP	denotes	]
T17731	9248-9249	,	denotes	,
T17732	9250-9253	CD	denotes	150
T17733	9254-9256	NNP	denotes	mM
T17734	9257-9261	NNP	denotes	NaCl
T17735	9261-9262	,	denotes	,
T17736	9263-9265	CD	denotes	10
T17737	9265-9266	NN	denotes	%
T17738	9267-9275	NN	denotes	glycerol
T17739	9275-9276	,	denotes	,
T17740	9277-9278	CD	denotes	5
T17741	9279-9281	NNP	denotes	mM
T17742	9282-9286	NNP	denotes	EDTA
T17743	9286-9287	,	denotes	,
T17744	9288-9289	CD	denotes	1
T17745	9289-9290	NN	denotes	%
T17746	9291-9296	NN	denotes	NP-40
T17747	9296-9297	-RRB-	denotes	)
T17748	9298-9300	IN	denotes	by
T17749	9301-9313	VBG	denotes	resuspending
T17750	9314-9321	NNS	denotes	samples
T17751	9322-9324	IN	denotes	in
T17752	9325-9327	CD	denotes	10
T17753	9328-9330	NN	denotes	μl
T17754	9331-9334	IN	denotes	per
T17755	9335-9338	CD	denotes	106
T17756	9339-9344	NNS	denotes	cells
T17757	9345-9348	CC	denotes	and
T17758	9349-9359	VBG	denotes	incubating
T17759	9360-9362	IN	denotes	on
T17760	9363-9366	NN	denotes	ice
T17761	9367-9370	IN	denotes	for
T17762	9371-9373	CD	denotes	30
T17763	9374-9377	NN	denotes	min
T17764	9378-9380	IN	denotes	in
T17765	9381-9384	DT	denotes	the
T17766	9385-9393	NN	denotes	presence
T17767	9394-9396	IN	denotes	of
T17768	9397-9405	NN	denotes	protease
T17769	9406-9416	NNS	denotes	inhibitors
T17770	9416-9417	.	denotes	.
T17771	9418-9428	NNP	denotes	Immunoblot
T17772	9429-9437	NN	denotes	analysis
T17773	9438-9441	VBD	denotes	was
T17774	9442-9451	VBN	denotes	performed
T17775	9452-9456	IN	denotes	with
T17776	9457-9460	DT	denotes	the
T17777	9461-9471	NNS	denotes	antibodies
T17778	9472-9481	VBD	denotes	indicated
T17779	9482-9484	IN	denotes	in
T17780	9485-9488	DT	denotes	the
T17781	9489-9496	NNS	denotes	figures
T17782	9497-9502	IN	denotes	after
T17783	9503-9511	NNP	denotes	SDS-PAGE
T17784	9512-9513	-LRB-	denotes	(
T17785	9513-9515	CD	denotes	10
T17786	9516-9518	CD	denotes	30
T17787	9519-9521	NN	denotes	μg
T17788	9522-9524	IN	denotes	of
T17789	9525-9530	JJ	denotes	total
T17790	9531-9538	NN	denotes	protein
T17791	9539-9542	VBD	denotes	was
T17792	9543-9549	VBN	denotes	loaded
T17793	9550-9553	IN	denotes	per
T17794	9554-9558	NN	denotes	well
T17795	9558-9559	-RRB-	denotes	)
T17796	9559-9560	.	denotes	.
T17797	9561-9575	NN	denotes	Quantification
T17798	9576-9578	IN	denotes	of
T17799	9579-9587	VBN	denotes	detected
T17800	9588-9595	NN	denotes	protein
T17801	9596-9599	VBD	denotes	was
T17802	9600-9609	VBN	denotes	performed
T17803	9610-9614	IN	denotes	with
T17804	9615-9617	DT	denotes	an
T17805	9618-9629	NNP	denotes	Intelligent
T17806	9630-9634	JJ	denotes	Dark
T17807	9635-9638	NNP	denotes	Box
T17808	9639-9643	NN	denotes	unit
T17809	9644-9645	-LRB-	denotes	(
T17810	9645-9653	NN	denotes	LAS-3000
T17811	9653-9654	:	denotes	;
T17812	9655-9663	NNP	denotes	Fujifilm
T17813	9663-9664	-RRB-	denotes	)
T17814	9665-9668	CC	denotes	and
T17815	9669-9679	VBN	denotes	normalized
T17816	9680-9683	IN	denotes	for
T17817	9684-9691	VBG	denotes	loading
T17818	9692-9696	IN	denotes	with
T17819	9697-9700	DT	denotes	the
T17820	9701-9707	NN	denotes	amount
T17821	9708-9710	IN	denotes	of
T17822	9711-9714	NNP	denotes	RNA
T17823	9715-9721	NNP	denotes	Pol-II
T17824	9722-9730	VBD	denotes	detected
T17825	9731-9733	IN	denotes	in
T17826	9734-9738	DT	denotes	each
T17827	9739-9743	NN	denotes	lane
T17828	9743-9744	.	denotes	.
T18126	9746-9752	NNP	denotes	Online
T18127	9753-9765	JJ	denotes	supplemental
T18128	9766-9774	NN	denotes	material
T18129	9774-9775	.	denotes	.
T18130	9776-9779	NNP	denotes	Fig
T18131	9779-9780	.	denotes	.
T18132	9781-9783	CD	denotes	S1
T18133	9784-9789	VBZ	denotes	shows
T18134	9790-9793	DT	denotes	the
T18135	9794-9810	NN	denotes	characterization
T18136	9811-9813	IN	denotes	of
T18137	9814-9824	JJ	denotes	peripheral
T18138	9825-9828	NNP	denotes	CD8
T18139	9828-9829	CD	denotes	+
T18140	9830-9831	NNP	denotes	T
T18141	9832-9837	NNS	denotes	cells
T18142	9838-9842	IN	denotes	from
T18143	9843-9848	JJ	denotes	Runx3
T18144	9848-9849	NN	denotes	−
T18145	9849-9850	NN	denotes	/
T18146	9850-9851	NN	denotes	−
T18147	9852-9856	NNS	denotes	mice
T18148	9856-9857	.	denotes	.
T18149	9858-9861	NNP	denotes	Fig
T18150	9861-9862	.	denotes	.
T18151	9863-9865	CD	denotes	S2
T18152	9866-9871	NNS	denotes	shows
T18153	9872-9880	JJ	denotes	effector
T18154	9881-9888	NN	denotes	protein
T18155	9889-9899	NN	denotes	expression
T18156	9900-9902	IN	denotes	by
T18157	9903-9908	NNP	denotes	Runx3
T18158	9909-9911	NNP	denotes	WT
T18159	9912-9915	CC	denotes	and
T18160	9916-9918	NNP	denotes	KO
T18161	9919-9924	NNS	denotes	cells
T18162	9925-9927	IN	denotes	at
T18163	9928-9931	NN	denotes	day
T18164	9932-9933	CD	denotes	4
T18165	9934-9936	IN	denotes	of
T18166	9937-9939	IN	denotes	in
T18167	9940-9945	NN	denotes	vitro
T18168	9946-9953	NN	denotes	culture
T18169	9953-9954	.	denotes	.
T18170	9955-9961	NNP	denotes	Primer
T18171	9962-9971	NNS	denotes	sequences
T18172	9972-9976	VBD	denotes	used
T18173	9977-9980	IN	denotes	for
T18174	9981-9985	NNP	denotes	ChIP
T18175	9986-9997	NNS	denotes	experiments
T18176	9998-10001	VBP	denotes	are
T18177	10002-10007	VBN	denotes	shown
T18178	10008-10010	IN	denotes	in
T18179	10011-10016	NNP	denotes	Table
T18180	10017-10019	NNP	denotes	S1
T18181	10019-10020	.	denotes	.
T18182	10021-10027	NNP	denotes	Online
T18183	10028-10040	JJ	denotes	supplemental
T18184	10041-10049	NN	denotes	material
T18185	10050-10052	VBZ	denotes	is
T18186	10053-10062	JJ	denotes	available
T18187	10063-10065	IN	denotes	at
T18188	10066-10118	NNP	denotes	http://www.jem.org/cgi/content/full/jem.20081242/DC1
T18189	10118-10119	.	denotes	.
T16704	5360-5363	IN	denotes	per
T16705	5364-5368	RB	denotes	well
T16706	5369-5371	IN	denotes	in
T16707	5372-5379	JJ	denotes	12-well
T16708	5380-5386	NNS	denotes	plates
T16709	5386-5387	,	denotes	,
T16710	5388-5391	DT	denotes	the
T16711	5392-5399	NN	denotes	culture
T16712	5400-5405	NNS	denotes	media
T16713	5406-5409	VBD	denotes	was
T16714	5410-5417	VBN	denotes	removed
T16715	5418-5421	CC	denotes	and
T16716	5422-5430	VBN	denotes	replaced
T16717	5431-5435	IN	denotes	with
T16718	5436-5444	JJ	denotes	complete
T16719	5445-5450	NNS	denotes	media
T16720	5451-5463	VBN	denotes	supplemented
T17572	8336-8344	NNP	denotes	Northern
T17573	8345-8348	CC	denotes	and
T17574	8349-8356	NNP	denotes	Western
T17575	8357-8361	NN	denotes	blot
T17576	8362-8370	NNS	denotes	analyses
T17577	8370-8371	.	denotes	.
T17578	8372-8375	NNP	denotes	RNA
T17579	8376-8385	NN	denotes	isolation
T17580	8386-8389	CC	denotes	and
T17581	8390-8398	JJ	denotes	Northern
T17582	8399-8403	NN	denotes	blot
T17583	8404-8412	NN	denotes	analysis
T17584	8413-8416	VBD	denotes	was
T17585	8417-8426	VBN	denotes	performed
T17586	8427-8429	IN	denotes	as
T17587	8430-8440	RB	denotes	previously
T17588	8441-8450	VBN	denotes	described
T17589	8451-8452	-LRB-	denotes	(
T17590	8452-8454	CD	denotes	29
T17591	8454-8455	-RRB-	denotes	)
T17592	8455-8456	.	denotes	.
T17593	8457-8459	IN	denotes	In
T17594	8460-8465	NN	denotes	brief
T17595	8465-8466	,	denotes	,
T17596	8467-8469	CD	denotes	10
T17597	8470-8472	NN	denotes	μg
T17598	8473-8475	IN	denotes	of
T17599	8476-8481	JJ	denotes	total
T17600	8482-8485	NNP	denotes	RNA
T17601	8486-8489	VBD	denotes	was
T17602	8490-8496	VBN	denotes	loaded
T17603	8497-8500	IN	denotes	per
T17604	8501-8505	NN	denotes	lane
T17605	8506-8509	CC	denotes	and
T17606	8510-8521	VBN	denotes	transferred
T17607	8522-8524	TO	denotes	to
T17608	8525-8535	RB	denotes	positively
T17609	8536-8543	VBN	denotes	charged
R10784	T13830	T13830	ROOT	Antibodies,Antibodies
R10785	T13831	T13830	cc	and,Antibodies
R10786	T13832	T13830	conj	reagents,Antibodies
R10787	T13833	T13830	punct	.,Antibodies
R10788	T13834	T13836	det	The,antibodies
R10789	T13835	T13836	amod	following,antibodies
R10790	T13836	T13844	nsubjpass	antibodies,obtained
R10791	T13837	T13836	acl	used,antibodies
R10792	T13838	T13837	prep	for,used
R10793	T13839	T13842	nmod	intracellular,stains
R10794	T13840	T13839	cc	or,intracellular
R10795	T13841	T13839	conj	surface,intracellular
R10796	T13842	T13838	pobj	stains,for
R10797	T13843	T13844	auxpass	were,obtained
R10798	T13844	T13844	ROOT	obtained,obtained
R10799	T13845	T13844	prep	from,obtained
R10800	T13846	T13845	pobj	eBioscience,from
R10801	T13847	T13844	punct	:,obtained
R10802	T13848	T13849	amod	anti,IL-2
R10803	T13849	T13849	ROOT	IL-2,IL-2
R10804	T13850	T13849	punct	",",IL-2
R10805	T13851	T13849	appos	anti,IL-2
R10806	T13852	T13858	nmod	IFN-γ,B
R10807	T13853	T13852	punct	",",IFN-γ
R10808	T13854	T13852	conj	anti-TNF,IFN-γ
R10809	T13855	T13856	punct	",",anti
R10810	T13856	T13858	amod	anti,B
R10811	T13857	T13858	compound	granzyme,B
R10812	T13858	T13849	appos	B,IL-2
R10813	T13859	T13858	punct	",",B
R10814	T13860	T13858	conj	anti-CD8,B
R10815	T13861	T13860	punct	",",anti-CD8
R10816	T13862	T13860	conj	anti-CD25,anti-CD8
R10817	T13863	T13862	punct	",",anti-CD25
R10818	T13864	T13862	cc	and,anti-CD25
R10819	T13865	T13862	conj	anti-CD44,anti-CD25
R10820	T13866	T13858	punct	.,B
R10821	T13867	T13869	nsubjpass	Anti-CD69,purchased
R10822	T13868	T13869	auxpass	was,purchased
R10823	T13869	T13869	ROOT	purchased,purchased
R10824	T13870	T13869	prep	from,purchased
R10825	T13871	T13870	pobj	BD,from
R10826	T13872	T13869	punct	.,purchased
R10827	T13873	T13881	prep	For,obtained
R10828	T13874	T13875	compound	ChIP,experiments
R10829	T13875	T13873	pobj	experiments,For
R10830	T13876	T13881	punct	",",obtained
R10831	T13877	T13879	det	the,antibody
R10832	T13878	T13879	amod	anti-Eomes,antibody
R10833	T13879	T13881	nsubjpass	antibody,obtained
R10834	T13880	T13881	auxpass	was,obtained
R10835	T13881	T13881	ROOT	obtained,obtained
R10836	T13882	T13881	prep	from,obtained
R10837	T13883	T13882	pobj	Abcam,from
R10838	T13884	T13883	cc	and,Abcam
R10839	T13885	T13887	det	the,antibody
R10840	T13886	T13887	amod	anti-Runx3,antibody
R10841	T13887	T13883	conj	antibody,Abcam
R10842	T13888	T13889	auxpass	was,produced
R10843	T13889	T13881	conj	produced,obtained
R10844	T13890	T13889	agent	by,produced
R10845	T13891	T13893	det	the,laboratory
R10846	T13892	T13893	compound	Groner,laboratory
R10847	T13893	T13890	pobj	laboratory,by
R10848	T13894	T13881	punct	.,obtained
R10849	T13895	T13897	det	The,antibodies
R10850	T13896	T13897	amod	following,antibodies
R10851	T13897	T13899	nsubjpass	antibodies,used
R10852	T13898	T13899	auxpass	were,used
R10853	T13899	T13899	ROOT	used,used
R10854	T13900	T13899	prep	for,used
R10855	T13901	T13900	pcomp	immunoblotting,for
R10856	T13902	T13903	punct	:,antiperforin
R10857	T13903	T13899	npadvmod	antiperforin,used
R10858	T13904	T13905	punct	(,Abcam
R10859	T13905	T13903	appos	Abcam,antiperforin
R10860	T13906	T13905	punct	),Abcam
R10861	T13907	T13903	punct	",",antiperforin
R10862	T13908	T13903	appos	anti-Eomes,antiperforin
R10863	T13909	T13910	punct	(,Abcam
R10864	T13910	T13908	appos	Abcam,anti-Eomes
R10865	T13911	T13910	punct	),Abcam
R10866	T13912	T13908	punct	",",anti-Eomes
R10867	T13913	T13908	cc	and,anti-Eomes
R10868	T13914	T13908	conj	anti,anti-Eomes
R10869	T13915	T13921	compound	Pol-II,Inc.
R10870	T13916	T13919	punct	(,Biotechnology
R10871	T13917	T13918	compound	Santa,Cruz
R10872	T13918	T13919	compound	Cruz,Biotechnology
R10873	T13919	T13915	appos	Biotechnology,Pol-II
R10874	T13920	T13919	punct	",",Biotechnology
R10875	T13921	T13903	appos	Inc.,antiperforin
R10876	T13922	T13903	punct	),antiperforin
R10877	T13923	T13899	punct	.,used
R10878	T13924	T13926	det	The,antibody
R10879	T13925	T13926	compound	T-bet,antibody
R10880	T13926	T13928	nsubjpass	antibody,provided
R10881	T13927	T13928	auxpass	was,provided
R10882	T13928	T13928	ROOT	provided,provided
R10883	T13929	T13928	agent	by,provided
R10884	T13930	T13931	compound	L.,Glimcher
R10885	T13931	T13929	pobj	Glimcher,by
R10886	T13932	T13934	punct	(,School
R10887	T13933	T13934	compound	Harvard,School
R10888	T13934	T13934	ROOT	School,School
R10889	T13935	T13934	prep	of,School
R10890	T13936	T13937	compound	Public,Health
R10891	T13937	T13935	pobj	Health,of
R10892	T13938	T13934	punct	",",School
R10893	T13939	T13934	conj	Boston,School
R10894	T13940	T13939	punct	",",Boston
R10895	T13941	T13939	conj	MA,Boston
R10896	T13942	T13939	punct	),Boston
R10897	T13943	T13928	punct	.,provided
R10898	T13944	T13946	det	The,reagents
R10899	T13945	T13946	amod	following,reagents
R10900	T13946	T13948	nsubjpass	reagents,used
R10901	T13947	T13948	auxpass	were,used
R10902	T13948	T13948	ROOT	used,used
R10903	T13949	T13948	prep	for,used
R10904	T13950	T13951	det	the,experiments
R10905	T13951	T13949	pobj	experiments,for
R10906	T13952	T13951	acl	presented,experiments
R10907	T13953	T13952	prep	in,presented
R10908	T13954	T13955	det	this,report
R10909	T13955	T13953	pobj	report,in
R10910	T13956	T13951	punct	:,experiments
R10911	T13957	T13958	compound	Annexin,V
R10912	T13958	T13951	appos	V,experiments
R10913	T13959	T13962	compound	FITC,Kit
R10914	T13960	T13962	compound	Apoptosis,Kit
R10915	T13961	T13962	compound	Detection,Kit
R10916	T13962	T13958	conj	Kit,V
R10917	T13963	T13962	punct	(,Kit
R10918	T13964	T13962	appos	BD,Kit
R10919	T13965	T13962	punct	),Kit
R10920	T13966	T13962	punct	",",Kit
R10921	T13967	T13962	conj	CD8,Kit
R10922	T13968	T13970	amod	Negative,Kit
R10923	T13969	T13970	compound	Isolation,Kit
R10924	T13970	T13962	conj	Kit,Kit
R10925	T13971	T13970	punct	(,Kit
R10926	T13972	T13970	nmod	Invitrogen,Kit
R10927	T13973	T13970	punct	),Kit
R10928	T13974	T13970	punct	",",Kit
R10929	T13975	T13976	compound	CD8,MicroBeads
R10930	T13976	T13958	conj	MicroBeads,V
R10931	T13977	T13976	punct	(,MicroBeads
R10932	T13978	T13979	compound	Miltenyi,Biotec
R10933	T13979	T13976	appos	Biotec,MicroBeads
R10934	T13980	T13976	punct	),MicroBeads
R10935	T13981	T13958	punct	",",V
R10936	T13982	T13958	cc	and,V
R10937	T13983	T13985	compound	SYBR,PCR
R10938	T13984	T13985	compound	Green,PCR
R10939	T13985	T13987	compound	PCR,Reagents
R10940	T13986	T13987	compound	Core,Reagents
R10941	T13987	T13958	conj	Reagents,V
R10942	T13988	T13990	punct	(,Biosystems
R10943	T13989	T13990	compound	Applied,Biosystems
R10944	T13990	T13987	parataxis	Biosystems,Reagents
R10945	T13991	T13990	punct	),Biosystems
R10946	T13992	T13948	punct	.,used
R10947	T13993	T13995	det	The,peptide
R10948	T13994	T13995	amod	Gp33,peptide
R10949	T13995	T14000	nsubjpass	peptide,synthesized
R10950	T13996	T13995	punct	(,peptide
R10951	T13997	T13995	appos	KAVYNFATC,peptide
R10952	T13998	T13995	punct	),peptide
R10953	T13999	T14000	auxpass	was,synthesized
R10954	T14000	T14000	ROOT	synthesized,synthesized
R10955	T14001	T14000	agent	by,synthesized
R10956	T14002	T14006	det	the,Facility
R10957	T14003	T14004	compound	Tufts,University
R10958	T14004	T14006	compound	University,Facility
R10959	T14005	T14006	compound	Core,Facility
R10960	T14006	T14001	pobj	Facility,by
R10961	T14007	T14000	punct	",",synthesized
R10962	T14008	T14000	cc	and,synthesized
R10963	T14009	T14010	nummod	10,mM
R10964	T14010	T14015	nsubjpass	mM,prepared
R10965	T14011	T14010	prep	of,mM
R10966	T14012	T14013	compound	stock,solutions
R10967	T14013	T14011	pobj	solutions,of
R10968	T14014	T14015	auxpass	was,prepared
R10969	T14015	T14000	conj	prepared,synthesized
R10970	T14016	T14015	prep	in,prepared
R10971	T14017	T14016	pobj	DMSO,in
R10972	T14018	T14015	punct	.,prepared
R11155	T14382	T14382	ROOT	Isolation,Isolation
R11156	T14383	T14382	cc	and,Isolation
R11157	T14384	T14382	conj	culture,Isolation
R11158	T14385	T14382	prep	of,Isolation
R11159	T14386	T14390	amod	primary,cells
R11160	T14387	T14390	nmod	CD8,cells
R11161	T14388	T14390	nummod	+,cells
R11162	T14389	T14390	compound	T,cells
R11163	T14390	T14385	pobj	cells,of
R11164	T14391	T14382	punct	.,Isolation
R11165	T14392	T14395	nummod	CD8,cells
R11166	T14393	T14395	nummod	+,cells
R11167	T14394	T14395	compound	T,cells
R11168	T14395	T14426	nsubjpass	cells,purified
R11169	T14396	T14395	prep	from,cells
R11170	T14397	T14396	pobj	4,from
R11171	T14398	T14399	compound	8-wk-old,Tcra
R11172	T14399	T14426	nsubjpass	Tcra,purified
R11173	T14400	T14406	nmod	−,transgenic
R11174	T14401	T14406	nmod	/,transgenic
R11175	T14402	T14406	nmod	−,transgenic
R11176	T14403	T14406	nmod	×,transgenic
R11177	T14404	T14406	nmod	P14,transgenic
R11178	T14405	T14406	nmod	TCR,transgenic
R11179	T14406	T14412	nmod	transgenic,WT
R11180	T14407	T14408	punct	(,Taconic
R11181	T14408	T14406	appos	Taconic,transgenic
R11182	T14409	T14408	punct	),Taconic
R11183	T14410	T14412	punct	",",WT
R11184	T14411	T14412	compound	C57BL/6J,WT
R11185	T14412	T14399	conj	WT,Tcra
R11186	T14413	T14412	punct	",",WT
R11187	T14414	T14412	cc	or,WT
R11188	T14415	T14418	nummod	Tbx21,−
R11189	T14416	T14418	nummod	−,−
R11190	T14417	T14418	compound	/,−
R11191	T14418	T14412	conj	−,WT
R11192	T14419	T14422	punct	(,Laboratory
R11193	T14420	T14422	det	The,Laboratory
R11194	T14421	T14422	compound	Jackson,Laboratory
R11195	T14422	T14418	appos	Laboratory,−
R11196	T14423	T14422	punct	),Laboratory
R11197	T14424	T14418	conj	mice,−
R11198	T14425	T14426	auxpass	were,purified
R11199	T14426	T14426	ROOT	purified,purified
R11200	T14427	T14426	punct	(,purified
R11201	T14428	T14431	compound	>,purity
R11202	T14429	T14430	nummod	95,%
R11203	T14430	T14431	compound	%,purity
R11204	T14431	T14426	appos	purity,purified
R11205	T14432	T14426	punct	),purified
R11206	T14433	T14426	agent	by,purified
R11207	T14434	T14435	amod	negative,selection
R11208	T14435	T14433	pobj	selection,by
R11209	T14436	T14437	punct	(,Invitrogen
R11210	T14437	T14435	appos	Invitrogen,selection
R11211	T14438	T14437	punct	),Invitrogen
R11212	T14439	T14435	prep	from,selection
R11213	T14440	T14441	amod	pooled,spleen
R11214	T14441	T14439	pobj	spleen,from
R11215	T14442	T14441	cc	and,spleen
R11216	T14443	T14445	amod	lymph,cells
R11217	T14444	T14445	compound	node,cells
R11218	T14445	T14441	conj	cells,spleen
R11219	T14446	T14426	punct	.,purified
R11220	T14447	T14450	nummod	CD8,cells
R11221	T14448	T14450	nummod	+,cells
R11222	T14449	T14450	compound	T,cells
R11223	T14450	T14462	nsubjpass	cells,purified
R11224	T14451	T14450	prep	from,cells
R11225	T14452	T14456	amod	Runx3,mice
R11226	T14453	T14456	nmod	−,mice
R11227	T14454	T14456	nmod	/,mice
R11228	T14455	T14456	compound	−,mice
R11229	T14456	T14451	pobj	mice,from
R11230	T14457	T14450	prep	on,cells
R11231	T14458	T14460	det	the,background
R11232	T14459	T14460	compound	ICR,background
R11233	T14460	T14457	pobj	background,on
R11234	T14461	T14462	auxpass	were,purified
R11235	T14462	T14462	ROOT	purified,purified
R11236	T14463	T14462	agent	by,purified
R11237	T14464	T14465	amod	positive,selection
R11238	T14465	T14463	pobj	selection,by
R11239	T14466	T14468	punct	(,Biotec
R11240	T14467	T14468	compound	Miltenyi,Biotec
R11241	T14468	T14465	appos	Biotec,selection
R11242	T14469	T14468	punct	),Biotec
R11243	T14470	T14462	punct	.,purified
R11244	T14471	T14472	det	All,mice
R11245	T14472	T14474	nsubjpass	mice,maintained
R11246	T14473	T14474	auxpass	were,maintained
R11247	T14474	T14474	ROOT	maintained,maintained
R11248	T14475	T14474	prep	in,maintained
R11249	T14476	T14479	amod	specific,facilities
R11250	T14477	T14479	amod	pathogen-free,facilities
R11251	T14478	T14479	compound	barrier,facilities
R11252	T14479	T14475	pobj	facilities,in
R11253	T14480	T14474	cc	and,maintained
R11254	T14481	T14474	conj	used,maintained
R11255	T14482	T14481	prep	according,used
R11256	T14483	T14482	prep	to,according
R11257	T14484	T14483	pobj	protocols,to
R11258	T14485	T14484	acl	approved,protocols
R11259	T14486	T14485	agent	by,approved
R11260	T14487	T14490	det	the,Institute
R11261	T14488	T14490	compound	Immune,Institute
R11262	T14489	T14490	compound	Disease,Institute
R11263	T14490	T14486	pobj	Institute,by
R11264	T14491	T14490	cc	and,Institute
R11265	T14492	T14497	det	the,Care
R11266	T14493	T14495	compound	Harvard,School
R11267	T14494	T14495	compound	Medical,School
R11268	T14495	T14497	compound	School,Care
R11269	T14496	T14497	compound	Animal,Care
R11270	T14497	T14490	conj	Care,Institute
R11271	T14498	T14497	cc	and,Care
R11272	T14499	T14497	conj	Use,Care
R11273	T14500	T14497	conj	Committees,Care
R11274	T14501	T14474	punct	.,maintained
R11275	T14502	T14511	prep	For,cultured
R11276	T14503	T14502	pobj	stimulation,For
R11277	T14504	T14511	punct	",",cultured
R11278	T14505	T14509	amod	purified,cells
R11279	T14506	T14509	nmod	CD8,cells
R11280	T14507	T14509	nummod	+,cells
R11281	T14508	T14509	compound	T,cells
R11282	T14509	T14511	nsubjpass	cells,cultured
R11283	T14510	T14511	auxpass	were,cultured
R11284	T14511	T14511	ROOT	cultured,cultured
R11285	T14512	T14511	prep	at,cultured
R11286	T14513	T14514	nummod	106,cells/ml
R11287	T14514	T14512	pobj	cells/ml,at
R11288	T14515	T14517	punct	(,ml
R11289	T14516	T14517	nummod	10,ml
R11290	T14517	T14514	appos	ml,cells/ml
R11291	T14518	T14517	punct	),ml
R11292	T14519	T14511	prep	in,cultured
R11293	T14520	T14521	nummod	T25,flasks
R11294	T14521	T14519	pobj	flasks,in
R11295	T14522	T14511	conj	coated,cultured
R11296	T14523	T14522	prep	with,coated
R11297	T14524	T14525	nummod	1,μg
R11298	T14525	T14527	compound	μg,ml
R11299	T14526	T14527	compound	/,ml
R11300	T14527	T14523	pobj	ml,with
R11301	T14528	T14527	appos	each,ml
R11302	T14529	T14528	prep	of,each
R11303	T14530	T14529	pobj	anti-CD3,of
R11304	T14531	T14533	punct	(,2C11
R11305	T14532	T14533	compound	clone,2C11
R11306	T14533	T14530	appos	2C11,anti-CD3
R11307	T14534	T14533	punct	),2C11
R11308	T14535	T14530	cc	and,anti-CD3
R11309	T14536	T14530	conj	anti-CD28,anti-CD3
R11310	T14537	T14538	punct	(,clone
R11311	T14538	T14536	appos	clone,anti-CD28
R11312	T14539	T14538	nummod	37.51,clone
R11313	T14540	T14538	punct	),clone
R11314	T14541	T14538	prep	by,clone
R11315	T14542	T14541	pobj	pretreatment,by
R11316	T14543	T14538	prep	with,clone
R11317	T14544	T14545	nummod	300,μg
R11318	T14545	T14549	nmod	μg,anti
R11319	T14546	T14549	nmod	/,anti
R11320	T14547	T14549	compound	ml,anti
R11321	T14548	T14549	compound	goat,anti
R11322	T14549	T14543	pobj	anti,with
R11323	T14550	T14551	compound	hamster,IgG
R11324	T14551	T14549	appos	IgG,anti
R11325	T14552	T14511	punct	.,cultured
R11326	T14553	T14559	prep	After,removed
R11327	T14554	T14555	nummod	48,h
R11328	T14555	T14553	pobj	h,After
R11329	T14556	T14559	punct	",",removed
R11330	T14557	T14559	nsubjpass	cells,removed
R11331	T14558	T14559	auxpass	were,removed
R11332	T14559	T14559	ROOT	removed,removed
R11333	T14560	T14559	prep	from,removed
R11334	T14561	T14563	det	the,stimulation
R11335	T14562	T14563	compound	TCR,stimulation
R11336	T14563	T14560	pobj	stimulation,from
R11337	T14564	T14559	cc	and,removed
R11338	T14565	T14559	conj	recultured,removed
R11339	T14566	T14565	prep	at,recultured
R11340	T14567	T14568	det	a,concentration
R11341	T14568	T14566	pobj	concentration,at
R11342	T14569	T14568	prep	of,concentration
R11343	T14570	T14573	nummod	5,cells/ml
R11344	T14571	T14573	nummod	×,cells/ml
R11345	T14572	T14573	nummod	105,cells/ml
R11346	T14573	T14569	pobj	cells/ml,of
R11347	T14574	T14568	prep	in,concentration
R11348	T14575	T14574	pobj	media,in
R11349	T14576	T14565	conj	supplemented,recultured
R11350	T14577	T14576	prep	with,supplemented
R11351	T14578	T14580	nummod	100,rhIL-2
R11352	T14579	T14580	compound	U/ml,rhIL-2
R11353	T14580	T14577	pobj	rhIL-2,with
R11354	T14581	T14559	punct	.,removed
R11355	T14582	T14584	det	Every,h
R11356	T14583	T14584	nummod	24,h
R11357	T14584	T14589	npadvmod	h,counted
R11358	T14585	T14589	punct	",",counted
R11359	T14586	T14587	amod	viable,cells
R11360	T14587	T14589	nsubjpass	cells,counted
R11361	T14588	T14589	auxpass	were,counted
R11362	T14589	T14589	ROOT	counted,counted
R11363	T14590	T14589	cc	and,counted
R11364	T14591	T14589	conj	readjusted,counted
R11365	T14592	T14591	prep	to,readjusted
R11366	T14593	T14596	nummod	5,cells/ml
R11367	T14594	T14596	nummod	×,cells/ml
R11368	T14595	T14596	nummod	105,cells/ml
R11369	T14596	T14592	pobj	cells/ml,to
R11370	T14597	T14591	prep	with,readjusted
R11371	T14598	T14599	amod	fresh,media
R11372	T14599	T14597	pobj	media,with
R11373	T14600	T14597	pcomp	containing,with
R11374	T14601	T14603	det	the,amount
R11375	T14602	T14603	amod	corresponding,amount
R11376	T14603	T14600	dobj	amount,containing
R11377	T14604	T14603	prep	of,amount
R11378	T14605	T14604	pobj	rhIL-2,of
R11379	T14606	T14589	punct	.,counted
R11619	T15057	T15057	ROOT	Isolation,Isolation
R11620	T15058	T15057	prep	of,Isolation
R11621	T15059	T15062	nummod	CD8,cells
R11622	T15060	T15062	nummod	+,cells
R11623	T15061	T15062	compound	T,cells
R11624	T15062	T15058	pobj	cells,of
R11625	T15063	T15057	prep	from,Isolation
R11626	T15064	T15068	amod	Runx3,mice
R11627	T15065	T15068	compound	−,mice
R11628	T15066	T15068	compound	/,mice
R11629	T15067	T15068	compound	−,mice
R11630	T15068	T15063	pobj	mice,from
R11631	T15069	T15057	punct	.,Isolation
R11632	T15070	T15072	amod	Runx3-deficient,cells
R11633	T15071	T15072	compound	T,cells
R11634	T15072	T15073	nsubj	cells,fail
R11635	T15073	T15073	ROOT	fail,fail
R11636	T15074	T15075	aux	to,silence
R11637	T15075	T15073	xcomp	silence,fail
R11638	T15076	T15077	compound	CD4,expression
R11639	T15077	T15075	dobj	expression,silence
R11640	T15078	T15075	advmod	normally,silence
R11641	T15079	T15080	punct	(,Fig
R11642	T15080	T15075	parataxis	Fig,silence
R11643	T15081	T15073	punct	.,fail
R11644	T15082	T15082	ROOT	S1,S1
R11645	T15083	T15082	punct	),S1
R11646	T15084	T15087	punct	(,13
R11647	T15085	T15087	nummod	12,13
R11648	T15086	T15087	punct	",",13
R11649	T15087	T15082	appos	13,S1
R11650	T15088	T15082	punct	),S1
R11651	T15089	T15082	punct	.,S1
R11652	T15090	T15093	nsubj	We,fractionated
R11653	T15091	T15093	advmod	therefore,fractionated
R11654	T15092	T15093	advmod	further,fractionated
R11655	T15093	T15093	ROOT	fractionated,fractionated
R11656	T15094	T15100	det	the,cells
R11657	T15095	T15096	advmod	positively,selected
R11658	T15096	T15100	amod	selected,cells
R11659	T15097	T15100	compound	CD8,cells
R11660	T15098	T15100	nummod	+,cells
R11661	T15099	T15100	compound	T,cells
R11662	T15100	T15093	dobj	cells,fractionated
R11663	T15101	T15100	prep	from,cells
R11664	T15102	T15103	compound	Runx3,KO
R11665	T15103	T15104	compound	KO,mice
R11666	T15104	T15101	pobj	mice,from
R11667	T15105	T15093	prep	into,fractionated
R11668	T15106	T15110	nummod	CD8,SP
R11669	T15107	T15110	nummod	+,SP
R11670	T15108	T15110	compound	CD4,SP
R11671	T15109	T15110	compound	−,SP
R11672	T15110	T15117	nmod	SP,cells
R11673	T15111	T15110	cc	or,SP
R11674	T15112	T15113	compound	CD8,+
R11675	T15113	T15117	nmod	+,cells
R11676	T15114	T15117	compound	CD4,cells
R11677	T15115	T15117	compound	+,cells
R11678	T15116	T15117	compound	DP,cells
R11679	T15117	T15105	pobj	cells,into
R11680	T15118	T15093	prep	by,fractionated
R11681	T15119	T15118	pobj	separation,by
R11682	T15120	T15093	advcl	using,fractionated
R11683	T15121	T15123	amod	anti-CD4,beads
R11684	T15122	T15123	amod	magnetic,beads
R11685	T15123	T15120	dobj	beads,using
R11686	T15124	T15093	punct	.,fractionated
R11687	T15125	T15126	nsubj	This,yielded
R11688	T15126	T15126	ROOT	yielded,yielded
R11689	T15127	T15129	det	a,KO
R11690	T15128	T15129	amod	Runx3,KO
R11691	T15129	T15130	compound	KO,SP
R11692	T15130	T15131	nsubj	SP,enriched
R11693	T15131	T15132	amod	enriched,population
R11694	T15132	T15126	dobj	population,yielded
R11695	T15133	T15134	nsubj	that,contained
R11696	T15134	T15132	relcl	contained,population
R11697	T15135	T15136	nummod	75,%
R11698	T15136	T15134	dobj	%,contained
R11699	T15137	T15141	nummod	CD8,cells
R11700	T15138	T15141	nmod	+,cells
R11701	T15139	T15141	nummod	CD4,cells
R11702	T15140	T15141	compound	−,cells
R11703	T15141	T15136	appos	cells,%
R11704	T15142	T15141	cc	and,cells
R11705	T15143	T15147	det	a,population
R11706	T15144	T15145	compound	KO,DP
R11707	T15145	T15147	nmod	DP,population
R11708	T15146	T15147	amod	enriched,population
R11709	T15147	T15141	conj	population,cells
R11710	T15148	T15149	nsubj	that,contained
R11711	T15149	T15147	relcl	contained,population
R11712	T15150	T15151	nummod	85,%
R11713	T15151	T15149	dobj	%,contained
R11714	T15152	T15156	nummod	CD8,cells
R11715	T15153	T15156	nmod	+,cells
R11716	T15154	T15156	nummod	CD4,cells
R11717	T15155	T15156	compound	+,cells
R11718	T15156	T15151	appos	cells,%
R11719	T15157	T15158	punct	(,Fig
R11720	T15158	T15156	appos	Fig,cells
R11721	T15159	T15126	punct	.,yielded
R11722	T15160	T15160	ROOT	S1,S1
R11723	T15161	T15160	punct	),S1
R11724	T15162	T15160	punct	.,S1
R11725	T15163	T15164	det	The,cells
R11726	T15164	T15166	nsubjpass	cells,stimulated
R11727	T15165	T15166	auxpass	were,stimulated
R11728	T15166	T15166	ROOT	stimulated,stimulated
R11729	T15167	T15166	prep	with,stimulated
R11730	T15168	T15170	amod	anti-CD3,anti-CD28
R11731	T15169	T15170	compound	+,anti-CD28
R11732	T15170	T15167	pobj	anti-CD28,with
R11733	T15171	T15166	prep	for,stimulated
R11734	T15172	T15173	nummod	2,d
R11735	T15173	T15171	pobj	d,for
R11736	T15174	T15166	prep	before,stimulated
R11737	T15175	T15174	pcomp	removing,before
R11738	T15176	T15175	dobj	them,removing
R11739	T15177	T15175	prep	from,removing
R11740	T15178	T15180	det	the,stimulus
R11741	T15179	T15180	compound	TCR,stimulus
R11742	T15180	T15177	pobj	stimulus,from
R11743	T15181	T15175	cc	and,removing
R11744	T15182	T15175	conj	culturing,removing
R11745	T15183	T15182	dobj	them,culturing
R11746	T15184	T15182	prep	in,culturing
R11747	T15185	T15184	pobj	media,in
R11748	T15186	T15185	acl	containing,media
R11749	T15187	T15189	nummod	100,IL-2
R11750	T15188	T15189	compound	U/ml,IL-2
R11751	T15189	T15186	dobj	IL-2,containing
R11752	T15190	T15166	punct	.,stimulated
R11753	T15191	T15193	mark	As,reported
R11754	T15192	T15193	advmod	previously,reported
R11755	T15193	T15208	advcl	reported,impaired
R11756	T15194	T15208	punct	",",impaired
R11757	T15195	T15196	amod	TCR-induced,proliferation
R11758	T15196	T15208	nsubjpass	proliferation,impaired
R11759	T15197	T15196	prep	of,proliferation
R11760	T15198	T15205	nmod	Runx3,cells
R11761	T15199	T15205	nmod	−,cells
R11762	T15200	T15205	nmod	/,cells
R11763	T15201	T15205	nmod	−,cells
R11764	T15202	T15205	nmod	CD8,cells
R11765	T15203	T15205	nmod	+,cells
R11766	T15204	T15205	compound	T,cells
R11767	T15205	T15197	pobj	cells,of
R11768	T15206	T15208	auxpass	was,impaired
R11769	T15207	T15208	advmod	severely,impaired
R11770	T15208	T15208	ROOT	impaired,impaired
R11771	T15209	T15208	punct	",",impaired
R11772	T15210	T15208	advmod	irrespective,impaired
R11773	T15211	T15210	prep	of,irrespective
R11774	T15212	T15213	nummod	CD4,expression
R11775	T15213	T15211	pobj	expression,of
R11776	T15214	T15215	punct	(,Fig
R11777	T15215	T15213	appos	Fig,expression
R11778	T15216	T15208	punct	.,impaired
R11779	T15217	T15217	ROOT	S1,S1
R11780	T15218	T15217	punct	),S1
R11781	T15219	T15222	punct	(,13
R11782	T15220	T15222	nummod	12,13
R11783	T15221	T15222	punct	",",13
R11784	T15222	T15217	appos	13,S1
R11785	T15223	T15217	punct	),S1
R11786	T15224	T15217	punct	.,S1
R11787	T15225	T15230	advmod	However,/
R11788	T15226	T15230	punct	",",/
R11789	T15227	T15229	det	the,−
R11790	T15228	T15229	compound	Runx3,−
R11791	T15229	T15230	nsubj	−,/
R11792	T15230	T15230	ROOT	/,/
R11793	T15231	T15232	nummod	−,cells
R11794	T15232	T15233	nsubj	cells,showed
R11795	T15233	T15230	ccomp	showed,/
R11796	T15234	T15236	amod	cell-surface,patterns
R11797	T15235	T15236	compound	expression,patterns
R11798	T15236	T15233	dobj	patterns,showed
R11799	T15237	T15236	amod	indicative,patterns
R11800	T15238	T15237	prep	of,indicative
R11801	T15239	T15240	amod	activated,cells
R11802	T15240	T15238	pobj	cells,of
R11803	T15241	T15240	punct	",",cells
R11804	T15242	T15240	prep	including,cells
R11805	T15243	T15242	pobj	up-regulation,including
R11806	T15244	T15243	prep	of,up-regulation
R11807	T15245	T15244	pobj	CD25,of
R11808	T15246	T15245	cc	and,CD25
R11809	T15247	T15245	conj	CD69,CD25
R11810	T15248	T15249	punct	(,Fig
R11811	T15249	T15247	appos	Fig,CD69
R11812	T15250	T15230	punct	.,/
R11813	T15251	T15251	ROOT	S1,S1
R11814	T15252	T15251	punct	),S1
R11815	T15253	T15251	punct	.,S1
R11816	T15254	T15255	mark	As,expected
R11817	T15255	T15271	advcl	expected,responded
R11818	T15256	T15255	prep	from,expected
R11819	T15257	T15258	poss	their,ability
R11820	T15258	T15256	pobj	ability,from
R11821	T15259	T15260	aux	to,up-regulate
R11822	T15260	T15258	acl	up-regulate,ability
R11823	T15261	T15260	dobj	CD25,up-regulate
R11824	T15262	T15261	punct	",",CD25
R11825	T15263	T15271	nsubj	Runx3,responded
R11826	T15264	T15270	nmod	−,cells
R11827	T15265	T15270	nmod	/,cells
R11828	T15266	T15270	nmod	−,cells
R11829	T15267	T15270	nmod	CD8,cells
R11830	T15268	T15270	nmod	+,cells
R11831	T15269	T15270	compound	T,cells
R11832	T15270	T15271	nsubj	cells,responded
R11833	T15271	T15271	ROOT	responded,responded
R11834	T15272	T15271	prep	to,responded
R11835	T15273	T15272	pobj	IL-2,to
R11836	T15274	T15271	dobj	supplementation,responded
R11837	T15275	T15271	prep	after,responded
R11838	T15276	T15275	pobj	day,after
R11839	T15277	T15276	nummod	2,day
R11840	T15278	T15271	cc	and,responded
R11841	T15279	T15280	advmod	efficiently,expanded
R11842	T15280	T15271	conj	expanded,responded
R11843	T15281	T15280	prep	until,expanded
R11844	T15282	T15281	pobj	day,until
R11845	T15283	T15282	nummod	6,day
R11846	T15284	T15282	prep	of,day
R11847	T15285	T15287	det	the,period
R11848	T15286	T15287	compound	culture,period
R11849	T15287	T15284	pobj	period,of
R11850	T15288	T15282	punct	",",day
R11851	T15289	T15280	advmod	albeit,expanded
R11852	T15290	T15280	prep	at,expanded
R11853	T15291	T15292	amod	slower,rates
R11854	T15292	T15290	pobj	rates,at
R11855	T15293	T15271	prep	compared,responded
R11856	T15294	T15293	prep	with,compared
R11857	T15295	T15296	compound	WT,cells
R11858	T15296	T15294	pobj	cells,with
R11859	T15297	T15298	punct	(,Fig
R11860	T15298	T15296	appos	Fig,cells
R11861	T15299	T15271	punct	.,responded
R11862	T15300	T15300	ROOT	S1,S1
R11863	T15301	T15300	punct	),S1
R11864	T15302	T15300	punct	.,S1
R11865	T15303	T15311	mark	Although,silenced
R11866	T15304	T15305	det	a,fraction
R11867	T15305	T15311	nsubj	fraction,silenced
R11868	T15306	T15305	prep	of,fraction
R11869	T15307	T15310	det	the,cells
R11870	T15308	T15310	compound	KO,cells
R11871	T15309	T15310	compound	DP,cells
R11872	T15310	T15306	pobj	cells,of
R11873	T15311	T15326	advcl	silenced,remained
R11874	T15312	T15313	nummod	CD4,expression
R11875	T15313	T15311	dobj	expression,silenced
R11876	T15314	T15311	prep	after,silenced
R11877	T15315	T15314	pobj	activation,after
R11878	T15316	T15326	punct	",",remained
R11879	T15317	T15318	det	the,ratio
R11880	T15318	T15326	nsubj	ratio,remained
R11881	T15319	T15318	prep	of,ratio
R11882	T15320	T15321	compound	SP/DP,cells
R11883	T15321	T15319	pobj	cells,of
R11884	T15322	T15318	prep	in,ratio
R11885	T15323	T15325	det	each,population
R11886	T15324	T15325	amod	enriched,population
R11887	T15325	T15322	pobj	population,in
R11888	T15326	T15326	ROOT	remained,remained
R11889	T15327	T15326	acomp	constant,remained
R11890	T15328	T15326	advmod	thereafter,remained
R11891	T15329	T15326	punct	",",remained
R11892	T15330	T15326	cc	and,remained
R11893	T15331	T15334	nsubj	we,observe
R11894	T15332	T15334	aux	did,observe
R11895	T15333	T15334	neg	not,observe
R11896	T15334	T15326	conj	observe,remained
R11897	T15335	T15337	det	any,differences
R11898	T15336	T15337	amod	major,differences
R11899	T15337	T15334	dobj	differences,observe
R11900	T15338	T15337	prep	between,differences
R11901	T15339	T15341	det	these,populations
R11902	T15340	T15341	nummod	two,populations
R11903	T15341	T15338	pobj	populations,between
R11904	T15342	T15334	prep	throughout,observe
R11905	T15343	T15345	det	the,period
R11906	T15344	T15345	compound	culture,period
R11907	T15345	T15342	pobj	period,throughout
R11908	T15346	T15334	punct	",",observe
R11909	T15347	T15334	advcl	indicating,observe
R11910	T15348	T15373	mark	that,are
R11911	T15349	T15373	prep	in,are
R11912	T15350	T15349	pobj	terms,in
R11913	T15351	T15350	prep	of,terms
R11914	T15352	T15354	compound	effector,differentiation
R11915	T15353	T15354	compound	CTL,differentiation
R11916	T15354	T15351	pobj	differentiation,of
R11917	T15355	T15349	cc	and,in
R11918	T15356	T15349	conj	under,in
R11919	T15357	T15359	poss	our,conditions
R11920	T15358	T15359	compound	culture,conditions
R11921	T15359	T15356	pobj	conditions,under
R11922	T15360	T15368	punct	",",cells
R11923	T15361	T15368	amod	Runx3,cells
R11924	T15362	T15366	nmod	−,+
R11925	T15363	T15366	nmod	/,+
R11926	T15364	T15366	nmod	−,+
R11927	T15365	T15366	nmod	CD8,+
R11928	T15366	T15368	nmod	+,cells
R11929	T15367	T15368	compound	T,cells
R11930	T15368	T15373	nsubj	cells,are
R11931	T15369	T15371	nsubj	that,coexpress
R11932	T15370	T15371	advmod	also,coexpress
R11933	T15371	T15368	relcl	coexpress,cells
R11934	T15372	T15371	dobj	CD4,coexpress
R11935	T15373	T15347	ccomp	are,indicating
R11936	T15374	T15373	acomp	indistinguishable,are
R11937	T15375	T15374	prep	from,indistinguishable
R11938	T15376	T15375	pobj	those,from
R11939	T15377	T15378	nsubj	that,do
R11940	T15378	T15376	relcl	do,those
R11941	T15379	T15378	neg	not,do
R11942	T15380	T15334	punct	.,observe
R11943	T15381	T15382	det	The,data
R11944	T15382	T15382	ROOT	data,data
R11945	T15383	T15382	acl	presented,data
R11946	T15384	T15383	prep	in,presented
R11947	T15385	T15384	pobj	Fig,in
R11948	T15386	T15383	punct	.,presented
R11949	T15387	T15388	nsubj	S2,are
R11950	T15388	T15388	ROOT	are,are
R11951	T15389	T15388	prep	from,are
R11952	T15390	T15391	amod	Runx3,KO
R11953	T15391	T15393	compound	KO,cells
R11954	T15392	T15393	compound	SP,cells
R11955	T15393	T15389	pobj	cells,from
R11956	T15394	T15388	punct	",",are
R11957	T15395	T15403	mark	whereas,are
R11958	T15396	T15403	nsubj	those,are
R11959	T15397	T15396	acl	shown,those
R11960	T15398	T15397	prep	in,shown
R11961	T15399	T15398	pobj	Figs.,in
R11962	T15400	T15399	nummod	3,Figs.
R11963	T15401	T15397	cc	and,shown
R11964	T15402	T15403	nsubj	4,are
R11965	T15403	T15388	advcl	are,are
R11966	T15404	T15403	prep	from,are
R11967	T15405	T15407	amod	total,KO
R11968	T15406	T15407	compound	Runx3,KO
R11969	T15407	T15409	compound	KO,cells
R11970	T15408	T15409	compound	CD8,cells
R11971	T15409	T15404	pobj	cells,from
R11972	T15410	T15388	punct	.,are
R12352	T16130	T16132	amod	FACS-based,assay
R12353	T16131	T16132	compound	cytotoxicity,assay
R12354	T16132	T16132	ROOT	assay,assay
R12355	T16133	T16132	punct	.,assay
R12356	T16134	T16135	aux	To,measure
R12357	T16135	T16143	advcl	measure,loaded
R12358	T16136	T16135	dobj	cytotoxicity,measure
R12359	T16137	T16143	punct	",",loaded
R12360	T16138	T16141	nummod	EL4,cells
R12361	T16139	T16141	compound	thymoma,cells
R12362	T16140	T16141	compound	target,cells
R12363	T16141	T16143	nsubjpass	cells,loaded
R12364	T16142	T16143	auxpass	were,loaded
R12365	T16143	T16143	ROOT	loaded,loaded
R12366	T16144	T16143	prep	with,loaded
R12367	T16145	T16144	pobj	0,with
R12368	T16146	T16145	cc	or,0
R12369	T16147	T16145	conj	1,0
R12370	T16148	T16150	compound	μM,peptide
R12371	T16149	T16150	compound	Gp33,peptide
R12372	T16150	T16145	appos	peptide,0
R12373	T16151	T16150	prep	for,peptide
R12374	T16152	T16153	nummod	2,h
R12375	T16153	T16151	pobj	h,for
R12376	T16154	T16143	prep	before,loaded
R12377	T16155	T16157	det	a,coincubation
R12378	T16156	T16157	amod	2-h,coincubation
R12379	T16157	T16154	pobj	coincubation,before
R12380	T16158	T16157	prep	with,coincubation
R12381	T16159	T16160	compound	P14,CD8
R12382	T16160	T16158	pobj	CD8,with
R12383	T16161	T16143	advcl	+,loaded
R12384	T16162	T16163	compound	T,cells
R12385	T16163	T16161	dobj	cells,+
R12386	T16164	T16161	prep	at,+
R12387	T16165	T16167	det	the,ratios
R12388	T16166	T16167	amod	effector-to-target,ratios
R12389	T16167	T16164	pobj	ratios,at
R12390	T16168	T16167	acl	indicated,ratios
R12391	T16169	T16168	prep	in,indicated
R12392	T16170	T16171	det	the,figures
R12393	T16171	T16169	pobj	figures,in
R12394	T16172	T16171	prep	in,figures
R12395	T16173	T16175	amod	96-well,plates
R12396	T16174	T16175	compound	round-bottom,plates
R12397	T16175	T16172	pobj	plates,in
R12398	T16176	T16143	punct	.,loaded
R12399	T16177	T16184	prep	After,stained
R12400	T16178	T16180	det	the,period
R12401	T16179	T16180	compound	coincubation,period
R12402	T16180	T16177	pobj	period,After
R12403	T16181	T16184	punct	",",stained
R12404	T16182	T16184	nsubjpass	cells,stained
R12405	T16183	T16184	auxpass	were,stained
R12406	T16184	T16184	ROOT	stained,stained
R12407	T16185	T16184	prep	with,stained
R12408	T16186	T16187	compound	Annexin,V
R12409	T16187	T16185	pobj	V,with
R12410	T16188	T16187	conj	FITC,V
R12411	T16189	T16188	cc	and,FITC
R12412	T16190	T16188	conj	anti-CD8,FITC
R12413	T16191	T16184	npadvmod	allophycocyanin,stained
R12414	T16192	T16184	punct	.,stained
R12415	T16193	T16194	compound	Data,analysis
R12416	T16194	T16196	nsubjpass	analysis,performed
R12417	T16195	T16196	auxpass	was,performed
R12418	T16196	T16214	ccomp	performed,were
R12419	T16197	T16196	prep	with,performed
R12420	T16198	T16199	compound	FlowJo,software
R12421	T16199	T16197	pobj	software,with
R12422	T16200	T16199	punct	(,software
R12423	T16201	T16202	compound	Tree,Star
R12424	T16202	T16199	appos	Star,software
R12425	T16203	T16204	punct	",",Inc.
R12426	T16204	T16199	appos	Inc.,software
R12427	T16205	T16196	punct	),performed
R12428	T16206	T16214	punct	;,were
R12429	T16207	T16209	nummod	EL4,cells
R12430	T16208	T16209	compound	target,cells
R12431	T16209	T16214	nsubj	cells,were
R12432	T16210	T16209	punct	(,cells
R12433	T16211	T16212	amod	CD8-negative,events
R12434	T16212	T16209	appos	events,cells
R12435	T16213	T16209	punct	),cells
R12436	T16214	T16214	ROOT	were,were
R12437	T16215	T16214	acomp	gated,were
R12438	T16216	T16214	punct	",",were
R12439	T16217	T16214	cc	and,were
R12440	T16218	T16219	det	the,percentage
R12441	T16219	T16227	nsubjpass	percentage,determined
R12442	T16220	T16219	prep	of,percentage
R12443	T16221	T16222	compound	Annexin,V
R12444	T16222	T16220	pobj	V,of
R12445	T16223	T16225	amod	+,cells
R12446	T16224	T16225	compound	target,cells
R12447	T16225	T16219	conj	cells,percentage
R12448	T16226	T16227	auxpass	was,determined
R12449	T16227	T16214	conj	determined,were
R12450	T16228	T16227	punct	.,determined
R12545	T16385	T16385	ROOT	Cytokine,Cytokine
R12546	T16386	T16385	cc	and,Cytokine
R12547	T16387	T16388	compound	surface,marker
R12548	T16388	T16385	conj	marker,Cytokine
R12549	T16389	T16385	acl	staining,Cytokine
R12550	T16390	T16385	punct	.,Cytokine
R12551	T16391	T16392	aux	To,assess
R12552	T16392	T16398	advcl	assess,restimulated
R12553	T16393	T16394	amod	cytokine,production
R12554	T16394	T16392	dobj	production,assess
R12555	T16395	T16398	punct	",",restimulated
R12556	T16396	T16398	nsubjpass	cells,restimulated
R12557	T16397	T16398	auxpass	were,restimulated
R12558	T16398	T16398	ROOT	restimulated,restimulated
R12559	T16399	T16398	prep	with,restimulated
R12560	T16400	T16403	nummod	10,+
R12561	T16401	T16402	compound	nM,PMA
R12562	T16402	T16403	nsubj	PMA,+
R12563	T16403	T16398	advcl	+,restimulated
R12564	T16404	T16405	nummod	1,μM
R12565	T16405	T16406	compound	μM,ionomycin
R12566	T16406	T16403	dobj	ionomycin,+
R12567	T16407	T16403	prep	for,+
R12568	T16408	T16409	nummod	6,h
R12569	T16409	T16407	pobj	h,for
R12570	T16410	T16412	punct	(,indicated
R12571	T16411	T16412	mark	unless,indicated
R12572	T16412	T16403	advcl	indicated,+
R12573	T16413	T16412	advmod	otherwise,indicated
R12574	T16414	T16412	prep	in,indicated
R12575	T16415	T16416	det	the,figures
R12576	T16416	T16414	pobj	figures,in
R12577	T16417	T16412	punct	),indicated
R12578	T16418	T16412	punct	",",indicated
R12579	T16419	T16412	cc	and,indicated
R12580	T16420	T16422	amod	intracellular,stains
R12581	T16421	T16422	compound	cytokine,stains
R12582	T16422	T16424	nsubjpass	stains,performed
R12583	T16423	T16424	auxpass	were,performed
R12584	T16424	T16412	conj	performed,indicated
R12585	T16425	T16427	mark	as,described
R12586	T16426	T16427	advmod	previously,described
R12587	T16427	T16424	advcl	described,performed
R12588	T16428	T16427	punct	(,described
R12589	T16429	T16427	npadvmod	28,described
R12590	T16430	T16427	punct	),described
R12591	T16431	T16403	punct	.,+
R12592	T16432	T16433	aux	To,detect
R12593	T16433	T16441	advcl	detect,washed
R12594	T16434	T16433	dobj	expression,detect
R12595	T16435	T16434	prep	of,expression
R12596	T16436	T16437	compound	surface,molecules
R12597	T16437	T16435	pobj	molecules,of
R12598	T16438	T16441	punct	",",washed
R12599	T16439	T16441	nsubjpass	cells,washed
R12600	T16440	T16441	auxpass	were,washed
R12601	T16441	T16441	ROOT	washed,washed
R12602	T16442	T16441	prep	in,washed
R12603	T16443	T16442	pobj	PBS,in
R12604	T16444	T16441	punct	",",washed
R12605	T16445	T16441	conj	resuspended,washed
R12606	T16446	T16448	mark	in,wash
R12607	T16447	T16448	nsubj	FACS,wash
R12608	T16448	T16471	meta	wash,indicated
R12609	T16449	T16448	dobj	buffer,wash
R12610	T16450	T16453	punct	(,FBS
R12611	T16451	T16452	nummod	3,%
R12612	T16452	T16453	npadvmod	%,FBS
R12613	T16453	T16471	nsubj	FBS,indicated
R12614	T16454	T16453	punct	",",FBS
R12615	T16455	T16456	nummod	0.1,%
R12616	T16456	T16458	compound	%,azide
R12617	T16457	T16458	compound	sodium,azide
R12618	T16458	T16453	appos	azide,FBS
R12619	T16459	T16458	punct	",",azide
R12620	T16460	T16462	nummod	30,Hepes
R12621	T16461	T16462	compound	mM,Hepes
R12622	T16462	T16458	appos	Hepes,azide
R12623	T16463	T16462	punct	",",Hepes
R12624	T16464	T16466	nummod	1,PBS
R12625	T16465	T16466	nummod	×,PBS
R12626	T16466	T16462	appos	PBS,Hepes
R12627	T16467	T16462	punct	),Hepes
R12628	T16468	T16458	acl	containing,azide
R12629	T16469	T16470	det	the,antibodies
R12630	T16470	T16468	dobj	antibodies,containing
R12631	T16471	T16441	conj	indicated,washed
R12632	T16472	T16471	prep	in,indicated
R12633	T16473	T16474	det	the,figures
R12634	T16474	T16472	pobj	figures,in
R12635	T16475	T16471	prep	at,indicated
R12636	T16476	T16477	advmod	previously,optimized
R12637	T16477	T16478	amod	optimized,concentrations
R12638	T16478	T16475	pobj	concentrations,at
R12639	T16479	T16471	punct	",",indicated
R12640	T16480	T16491	nsubj	incubated,washed
R12641	T16481	T16480	prep	for,incubated
R12642	T16482	T16483	nummod	15,min
R12643	T16483	T16481	pobj	min,for
R12644	T16484	T16483	prep	at,min
R12645	T16485	T16486	compound	room,temperature
R12646	T16486	T16484	pobj	temperature,at
R12647	T16487	T16488	punct	(,RT
R12648	T16488	T16483	parataxis	RT,min
R12649	T16489	T16488	punct	),RT
R12650	T16490	T16483	punct	",",min
R12651	T16491	T16471	conj	washed,indicated
R12652	T16492	T16491	punct	",",washed
R12653	T16493	T16491	cc	and,washed
R12654	T16494	T16491	conj	resuspended,washed
R12655	T16495	T16494	prep	in,resuspended
R12656	T16496	T16497	nummod	2,%
R12657	T16497	T16500	nmod	%,solution
R12658	T16498	T16500	nmod	formaldehyde,solution
R12659	T16499	T16500	amod	fixative,solution
R12660	T16500	T16495	pobj	solution,in
R12661	T16501	T16500	prep	before,solution
R12662	T16502	T16501	pobj	acquisition,before
R12663	T16503	T16502	prep	on,acquisition
R12664	T16504	T16505	det	a,FACSCalibur
R12665	T16505	T16503	pobj	FACSCalibur,on
R12666	T16506	T16507	punct	(,BD
R12667	T16507	T16505	appos	BD,FACSCalibur
R12668	T16508	T16505	punct	),FACSCalibur
R12669	T16509	T16441	punct	.,washed
R12799	T16656	T16657	compound	Retroviral,transduction
R12800	T16657	T16657	ROOT	transduction,transduction
R12801	T16658	T16657	prep	of,transduction
R12802	T16659	T16663	amod	primary,cells
R12803	T16660	T16663	nmod	CD8,cells
R12804	T16661	T16663	nummod	+,cells
R12805	T16662	T16663	compound	T,cells
R12806	T16663	T16658	pobj	cells,of
R12807	T16664	T16657	punct	.,transduction
R12808	T16665	T16672	prep	For,generated
R12809	T16666	T16667	compound	transduction,experiments
R12810	T16667	T16665	pobj	experiments,For
R12811	T16668	T16672	punct	",",generated
R12812	T16669	T16670	amod	viral,supernatants
R12813	T16670	T16672	nsubjpass	supernatants,generated
R12814	T16671	T16672	auxpass	were,generated
R12815	T16672	T16672	ROOT	generated,generated
R12816	T16673	T16672	prep	by,generated
R12817	T16674	T16676	compound	calcium,transfection
R12818	T16675	T16676	compound	phosphate,transfection
R12819	T16676	T16673	pobj	transfection,by
R12820	T16677	T16676	prep	of,transfection
R12821	T16678	T16679	compound	Phoenix,cells
R12822	T16679	T16677	pobj	cells,of
R12823	T16680	T16679	cc	and,cells
R12824	T16681	T16676	conj	concentration,transfection
R12825	T16682	T16681	prep	by,concentration
R12826	T16683	T16684	amod	overnight,centrifugation
R12827	T16684	T16682	pobj	centrifugation,by
R12828	T16685	T16672	prep	at,generated
R12829	T16686	T16687	nummod	"6,000",g
R12830	T16687	T16685	pobj	g,at
R12831	T16688	T16672	punct	.,generated
R12832	T16689	T16714	prep	At,removed
R12833	T16690	T16714	nsubjpass	∼,removed
R12834	T16691	T16692	nummod	42,h
R12835	T16692	T16693	npadvmod	h,after
R12836	T16693	T16690	prep	after,∼
R12837	T16694	T16697	det	the,activation
R12838	T16695	T16697	amod	initial,activation
R12839	T16696	T16697	compound	TCR,activation
R12840	T16697	T16693	pobj	activation,after
R12841	T16698	T16697	prep	of,activation
R12842	T16699	T16703	nummod	106,cells
R12843	T16700	T16703	nummod	CD8,cells
R12844	T16701	T16703	compound	+,cells
R12845	T16702	T16703	compound	T,cells
R12846	T16703	T16698	pobj	cells,of
R12847	T16704	T16703	prep	per,cells
R12848	T16705	T16706	advmod	well,in
R12849	T16706	T16704	prep	in,per
R12850	T16707	T16708	amod	12-well,plates
R12851	T16708	T16706	pobj	plates,in
R12852	T16709	T16714	punct	",",removed
R12853	T16710	T16712	det	the,media
R12854	T16711	T16712	compound	culture,media
R12855	T16712	T16714	nsubjpass	media,removed
R12856	T16713	T16714	auxpass	was,removed
R12857	T16714	T16714	ROOT	removed,removed
R12858	T16715	T16714	cc	and,removed
R12859	T16716	T16714	conj	replaced,removed
R12860	T16717	T16716	prep	with,replaced
R12861	T16718	T16719	amod	complete,media
R12862	T16719	T16717	pobj	media,with
R12863	T16720	T16714	conj	supplemented,removed
R12864	T16721	T16720	prep	with,supplemented
R12865	T16722	T16723	nummod	8,μg
R12866	T16723	T16726	nmod	μg,polybrene
R12867	T16724	T16726	nmod	/,polybrene
R12868	T16725	T16726	compound	ml,polybrene
R12869	T16726	T16721	pobj	polybrene,with
R12870	T16727	T16726	acl	containing,polybrene
R12871	T16728	T16731	amod	fresh,virus
R12872	T16729	T16728	cc	plus,fresh
R12873	T16730	T16728	conj	concentrated,fresh
R12874	T16731	T16727	dobj	virus,containing
R12875	T16732	T16714	punct	.,removed
R12876	T16733	T16734	det	The,plates
R12877	T16734	T16736	nsubjpass	plates,centrifuged
R12878	T16735	T16736	auxpass	were,centrifuged
R12879	T16736	T16736	ROOT	centrifuged,centrifuged
R12880	T16737	T16736	prep	at,centrifuged
R12881	T16738	T16739	nummod	700,g
R12882	T16739	T16737	pobj	g,at
R12883	T16740	T16736	prep	for,centrifuged
R12884	T16741	T16742	nummod	1,h
R12885	T16742	T16740	pobj	h,for
R12886	T16743	T16742	prep	at,h
R12887	T16744	T16743	pobj	RT,at
R12888	T16745	T16736	prep	before,centrifuged
R12889	T16746	T16745	pcomp	returning,before
R12890	T16747	T16746	prep	to,returning
R12891	T16748	T16749	compound	37,°
R12892	T16749	T16750	nummod	°,C
R12893	T16750	T16747	pobj	C,to
R12894	T16751	T16746	prep	for,returning
R12895	T16752	T16757	det	an,constructs
R12896	T16753	T16757	amod	additional,constructs
R12897	T16754	T16755	nummod	5,h.
R12898	T16755	T16756	compound	h.,Retroviral
R12899	T16756	T16757	compound	Retroviral,constructs
R12900	T16757	T16751	pobj	constructs,for
R12901	T16758	T16757	prep	for,constructs
R12902	T16759	T16758	pobj	Eomes-VP16,for
R12903	T16760	T16759	cc	and,Eomes-VP16
R12904	T16761	T16765	det	the,vector
R12905	T16762	T16763	compound	MIG,control
R12906	T16763	T16765	nmod	control,vector
R12907	T16764	T16765	amod	empty,vector
R12908	T16765	T16759	conj	vector,Eomes-VP16
R12909	T16766	T16736	auxpass	were,centrifuged
R12910	T16767	T16768	det	a,gift
R12911	T16768	T16736	nsubjpass	gift,centrifuged
R12912	T16769	T16768	prep	from,gift
R12913	T16770	T16771	compound	S.L.,Reiner
R12914	T16771	T16769	pobj	Reiner,from
R12915	T16772	T16771	punct	(,Reiner
R12916	T16773	T16771	appos	University,Reiner
R12917	T16774	T16773	prep	of,University
R12918	T16775	T16774	pobj	Pennsylvania,of
R12919	T16776	T16775	punct	",",Pennsylvania
R12920	T16777	T16775	conj	Philadelphia,Pennsylvania
R12921	T16778	T16777	punct	",",Philadelphia
R12922	T16779	T16777	conj	PA,Philadelphia
R12923	T16780	T16771	punct	),Reiner
R12924	T16781	T16771	punct	(,Reiner
R12925	T16782	T16771	appos	8,Reiner
R12926	T16783	T16771	punct	),Reiner
R12927	T16784	T16736	punct	.,centrifuged
R13635	T17622	T17611	relcl	confirmed,membranes
R13636	T17623	T17622	agent	by,confirmed
R13637	T17624	T17626	amod	ethidium,staining
R13638	T17625	T17626	compound	bromide,staining
R13639	T17626	T17623	pobj	staining,by
R13640	T17627	T17626	prep	of,staining
R13641	T17628	T17630	amod	ribosomal,species
R13642	T17629	T17630	compound	RNA,species
R13643	T17630	T17627	pobj	species,of
R13644	T17631	T17626	prep	on,staining
R13645	T17632	T17633	det	the,membrane
R13646	T17633	T17631	pobj	membrane,on
R13647	T17634	T17602	punct	.,loaded
R13648	T17635	T17637	nsubjpass	Membranes,hybridized
R13649	T17636	T17637	auxpass	were,hybridized
R13650	T17637	T17646	ccomp	hybridized,dCTP
R13651	T17638	T17637	prep	with,hybridized
R13652	T17639	T17640	nummod	1,ng/ml
R13653	T17640	T17638	pobj	ng/ml,with
R13654	T17641	T17637	punct	α,hybridized
R13655	T17642	T17646	punct	-,dCTP
R13656	T17643	T17646	compound	[,dCTP
R13657	T17644	T17646	compound	32P,dCTP
R13658	T17645	T17646	compound	],dCTP
R13659	T17646	T17646	ROOT	dCTP,dCTP
R13660	T17647	T17646	acl	labeled,dCTP
R13661	T17648	T17649	amod	trichloroacetic,acid
R13662	T17649	T17651	nmod	acid,probe
R13663	T17650	T17651	amod	precipitable,probe
R13664	T17651	T17647	dobj	probe,labeled
R13665	T17652	T17647	prep	in,labeled
R13666	T17653	T17655	nmod	ExpressHyb,buffer
R13667	T17654	T17655	compound	hybridization,buffer
R13668	T17655	T17652	pobj	buffer,in
R13669	T17656	T17658	punct	(,Laboratories
R13670	T17657	T17658	compound	Clontech,Laboratories
R13671	T17658	T17655	appos	Laboratories,buffer
R13672	T17659	T17658	punct	",",Laboratories
R13673	T17660	T17658	appos	Inc.,Laboratories
R13674	T17661	T17658	punct	),Laboratories
R13675	T17662	T17646	punct	.,dCTP
R13676	T17663	T17665	det	All,probes
R13677	T17664	T17665	compound	cDNA,probes
R13678	T17665	T17667	nsubjpass	probes,confirmed
R13679	T17666	T17667	auxpass	were,confirmed
R13680	T17667	T17667	ROOT	confirmed,confirmed
R13681	T17668	T17669	aux	to,have
R13682	T17669	T17667	xcomp	have,confirmed
R13683	T17670	T17673	det	the,specificity
R13684	T17671	T17673	amod	appropriate,specificity
R13685	T17672	T17673	amod	single-copy,specificity
R13686	T17673	T17669	dobj	specificity,have
R13687	T17674	T17669	prep	under,have
R13688	T17675	T17676	det	these,conditions
R13689	T17676	T17674	pobj	conditions,under
R13690	T17677	T17669	advcl	using,have
R13691	T17678	T17681	amod	genomic,analysis
R13692	T17679	T17680	compound	Southern,blot
R13693	T17680	T17681	compound	blot,analysis
R13694	T17681	T17677	dobj	analysis,using
R13695	T17682	T17667	punct	.,confirmed
R13696	T17683	T17684	compound	Band,intensities
R13697	T17684	T17686	nsubjpass	intensities,acquired
R13698	T17685	T17686	auxpass	were,acquired
R13699	T17686	T17686	ROOT	acquired,acquired
R13700	T17687	T17686	agent	by,acquired
R13701	T17688	T17689	amod	phosphorimaging,analysis
R13702	T17689	T17687	pobj	analysis,by
R13703	T17690	T17686	punct	.,acquired
R13704	T17691	T17699	prep	For,obtained
R13705	T17692	T17693	amod	Western,analysis
R13706	T17693	T17691	pobj	analysis,For
R13707	T17694	T17699	punct	",",obtained
R13708	T17695	T17697	amod	whole-cell,lysates
R13709	T17696	T17697	compound	protein,lysates
R13710	T17697	T17699	nsubjpass	lysates,obtained
R13711	T17698	T17699	auxpass	were,obtained
R13712	T17699	T17699	ROOT	obtained,obtained
R13732	T17719	T17716	pobj	IL-2,in
R13733	T17720	T17719	prep	with,IL-2
R13749	T17736	T17737	nummod	10,%
R13750	T17737	T17738	compound	%,glycerol
R13751	T17738	T17734	appos	glycerol,NaCl
R13752	T17739	T17734	punct	",",NaCl
R13753	T17740	T17742	nummod	5,EDTA
R13754	T17741	T17742	compound	mM,EDTA
R13755	T17742	T17734	appos	EDTA,NaCl
R13756	T17743	T17742	punct	",",EDTA
R13757	T17744	T17745	nummod	1,%
R13758	T17745	T17746	compound	%,NP-40
R13759	T17746	T17742	appos	NP-40,EDTA
R13760	T17747	T17742	punct	),EDTA
R13761	T17748	T17709	prep	by,indicated
R13783	T17770	T17699	punct	.,obtained
R13784	T17771	T17772	compound	Immunoblot,analysis
R13785	T17772	T17774	nsubjpass	analysis,performed
R13786	T17773	T17774	auxpass	was,performed
R13787	T17774	T17774	ROOT	performed,performed
R13788	T17775	T17774	prep	with,performed
R13789	T17776	T17777	det	the,antibodies
R13790	T17777	T17775	pobj	antibodies,with
R13791	T17778	T17774	conj	indicated,performed
R13792	T17779	T17778	prep	in,indicated
R13793	T17780	T17781	det	the,figures
R13794	T17781	T17779	pobj	figures,in
R13795	T17782	T17792	mark	after,loaded
R13796	T17783	T17782	pobj	SDS-PAGE,after
R13797	T17784	T17783	punct	(,SDS-PAGE
R13798	T17785	T17787	nummod	10,μg
R13799	T17786	T17787	nummod	30,μg
R13800	T17787	T17783	appos	μg,SDS-PAGE
R13801	T17788	T17787	prep	of,μg
R13802	T17789	T17790	amod	total,protein
R13803	T17790	T17788	pobj	protein,of
R13804	T17791	T17792	auxpass	was,loaded
R13805	T17792	T17778	advcl	loaded,indicated
R13806	T17793	T17792	prep	per,loaded
R13807	T17794	T17793	pobj	well,per
R13808	T17795	T17778	punct	),indicated
R13809	T17796	T17774	punct	.,performed
R13810	T17797	T17802	nsubjpass	Quantification,performed
R13811	T17798	T17797	prep	of,Quantification
R13812	T17799	T17800	amod	detected,protein
R13813	T17800	T17798	pobj	protein,of
R13814	T17801	T17802	auxpass	was,performed
R13815	T17802	T17802	ROOT	performed,performed
R13816	T17803	T17802	prep	with,performed
R13817	T17804	T17808	det	an,unit
R13818	T17805	T17807	compound	Intelligent,Box
R13819	T17806	T17807	compound	Dark,Box
R13820	T17807	T17808	compound	Box,unit
R13821	T17808	T17803	pobj	unit,with
R13822	T17809	T17808	punct	(,unit
R13823	T17810	T17808	appos	LAS-3000,unit
R13824	T17811	T17812	punct	;,Fujifilm
R13825	T17812	T17802	npadvmod	Fujifilm,performed
R13826	T17813	T17812	punct	),Fujifilm
R13827	T17814	T17802	cc	and,performed
R13828	T17815	T17802	conj	normalized,performed
R13829	T17816	T17815	prep	for,normalized
R13830	T17817	T17816	pcomp	loading,for
R13831	T17818	T17817	prep	with,loading
R13832	T17819	T17820	det	the,amount
R13833	T17820	T17818	pobj	amount,with
R13834	T17821	T17820	prep	of,amount
R13835	T17822	T17823	compound	RNA,Pol-II
R13836	T17823	T17821	pobj	Pol-II,of
R13837	T17824	T17820	acl	detected,amount
R13838	T17825	T17824	prep	in,detected
R13839	T17826	T17827	det	each,lane
R13840	T17827	T17825	pobj	lane,in
R13841	T17828	T17802	punct	.,performed
R14094	T18126	T18128	amod	Online,material
R14095	T18127	T18128	amod	supplemental,material
R14096	T18128	T18128	ROOT	material,material
R14097	T18129	T18128	punct	.,material
R14098	T18130	T18130	ROOT	Fig,Fig
R14099	T18131	T18130	punct	.,Fig
R14100	T18132	T18133	nsubj	S1,shows
R14101	T18133	T18133	ROOT	shows,shows
R14102	T18134	T18135	det	the,characterization
R14103	T18135	T18133	dobj	characterization,shows
R14104	T18136	T18135	prep	of,characterization
R14105	T18137	T18141	amod	peripheral,cells
R14106	T18138	T18141	nmod	CD8,cells
R14107	T18139	T18141	nummod	+,cells
R14108	T18140	T18141	compound	T,cells
R14109	T18141	T18136	pobj	cells,of
R14110	T18142	T18135	prep	from,characterization
R14111	T18143	T18147	amod	Runx3,mice
R14112	T18144	T18147	compound	−,mice
R14113	T18145	T18147	compound	/,mice
R14114	T18146	T18147	compound	−,mice
R14115	T18147	T18142	pobj	mice,from
R14116	T18148	T18133	punct	.,shows
R14117	T18149	T18149	ROOT	Fig,Fig
R14118	T18150	T18149	punct	.,Fig
R14119	T18151	T18152	nummod	S2,shows
R14120	T18152	T18152	ROOT	shows,shows
R14121	T18153	T18155	amod	effector,expression
R14122	T18154	T18155	compound	protein,expression
R14123	T18155	T18152	dobj	expression,shows
R14124	T18156	T18152	prep	by,shows
R14125	T18157	T18158	compound	Runx3,WT
R14126	T18158	T18156	pobj	WT,by
R14127	T18159	T18158	cc	and,WT
R14128	T18160	T18158	conj	KO,WT
R14129	T18161	T18152	conj	cells,shows
R14130	T18162	T18152	prep	at,shows
R14131	T18163	T18162	pobj	day,at
R14132	T18164	T18163	nummod	4,day
R14133	T18165	T18163	prep	of,day
R14134	T18166	T18152	prep	in,shows
R14135	T18167	T18168	amod	vitro,culture
R14136	T18168	T18166	pobj	culture,in
R14137	T18169	T18152	punct	.,shows
R14138	T18170	T18171	compound	Primer,sequences
R14139	T18171	T18177	nsubjpass	sequences,shown
R14140	T18172	T18171	acl	used,sequences
R14141	T18173	T18172	prep	for,used
R14142	T18174	T18175	compound	ChIP,experiments
R14143	T18175	T18173	pobj	experiments,for
R14144	T18176	T18177	auxpass	are,shown
R14145	T18177	T18177	ROOT	shown,shown
R14146	T18178	T18177	prep	in,shown
R14147	T18179	T18180	compound	Table,S1
R14148	T18180	T18178	pobj	S1,in
R14149	T18181	T18177	punct	.,shown
R14150	T18182	T18184	amod	Online,material
R14151	T18183	T18184	amod	supplemental,material
R14152	T18184	T18185	nsubj	material,is
R14153	T18185	T18185	ROOT	is,is
R14154	T18186	T18185	acomp	available,is
R14155	T18187	T18186	prep	at,available
R14156	T18188	T18187	pobj	http://www.jem.org/cgi/content/full/jem.20081242/DC1,at
R14157	T18189	T18185	punct	.,is
R13713	T17700	T17699	prep	from,obtained
R13715	T17702	T17704	nummod	+,cells
R13716	T17703	T17704	compound	T,cells
R13717	T17704	T17700	pobj	cells,from
R13718	T17705	T17699	prep	at,obtained
R13719	T17706	T17707	det	the,time
R13720	T17707	T17705	pobj	time,at
R13721	T17708	T17709	nsubj	points,indicated
R13722	T17709	T17707	relcl	indicated,time
R13723	T17710	T17709	prep	in,indicated
R13724	T17711	T17712	det	the,figures
R13725	T17712	T17710	pobj	figures,in
R13726	T17713	T17709	prep	during,indicated
R13727	T17714	T17715	amod	clonal,expansion
R13728	T17715	T17713	pobj	expansion,during
R13729	T17716	T17715	prep	in,expansion
R13730	T17717	T17719	nummod	100,IL-2
R13731	T17718	T17719	compound	U/ml,IL-2
R13734	T17721	T17722	compound	lysis,buffer
R13735	T17722	T17720	pobj	buffer,with
R13736	T17723	T17730	punct	(,]
R13737	T17724	T17730	nummod	50,]
R13738	T17725	T17728	compound	mM,pH
R13739	T17726	T17728	compound	Tris,pH
R13740	T17727	T17728	compound	[,pH
R13741	T17728	T17730	compound	pH,]
R13742	T17729	T17730	compound	7.5,]
R13743	T17730	T17722	appos	],buffer
R13744	T17731	T17730	punct	",",]
R13745	T17732	T17734	nummod	150,NaCl
R13746	T17733	T17734	compound	mM,NaCl
R13747	T17734	T17730	appos	NaCl,]
R13748	T17735	T17734	punct	",",NaCl
R13762	T17749	T17748	pcomp	resuspending,by
R13763	T17750	T17749	dobj	samples,resuspending
R13764	T17751	T17749	prep	in,resuspending
R13765	T17752	T17753	nummod	10,μl
R13766	T17753	T17751	pobj	μl,in
R13767	T17754	T17753	prep	per,μl
R13768	T17755	T17756	nummod	106,cells
R13769	T17756	T17754	pobj	cells,per
R13770	T17757	T17749	cc	and,resuspending
R13771	T17758	T17749	conj	incubating,resuspending
R13772	T17759	T17758	prep	on,incubating
R13773	T17760	T17759	pobj	ice,on
R13774	T17761	T17758	prep	for,incubating
R13775	T17762	T17763	nummod	30,min
R13776	T17763	T17761	pobj	min,for
R13777	T17764	T17763	prep	in,min
R13778	T17765	T17766	det	the,presence
R13779	T17766	T17764	pobj	presence,in
R13780	T17767	T17766	prep	of,presence
R13781	T17768	T17769	compound	protease,inhibitors
R13782	T17769	T17767	pobj	inhibitors,of
R13585	T17572	T17576	nmod	Northern,analyses
R13586	T17573	T17572	cc	and,Northern
R13587	T17574	T17572	conj	Western,Northern
R13588	T17575	T17576	compound	blot,analyses
R13589	T17576	T17576	ROOT	analyses,analyses
R13590	T17577	T17576	punct	.,analyses
R13591	T17578	T17579	compound	RNA,isolation
R13592	T17579	T17585	nsubjpass	isolation,performed
R13593	T17580	T17579	cc	and,isolation
R13594	T17581	T17582	amod	Northern,blot
R13595	T17582	T17583	compound	blot,analysis
R13596	T17583	T17579	conj	analysis,isolation
R13597	T17584	T17585	auxpass	was,performed
R13598	T17585	T17585	ROOT	performed,performed
R13599	T17586	T17588	mark	as,described
R13600	T17587	T17588	advmod	previously,described
R13601	T17588	T17585	advcl	described,performed
R13602	T17589	T17588	punct	(,described
R13603	T17590	T17588	npadvmod	29,described
R13604	T17591	T17588	punct	),described
R13605	T17592	T17585	punct	.,performed
R13606	T17593	T17602	prep	In,loaded
R13607	T17594	T17593	pobj	brief,In
R13608	T17595	T17602	punct	",",loaded
R13609	T17596	T17597	nummod	10,μg
R13610	T17597	T17602	nsubjpass	μg,loaded
R13611	T17598	T17597	prep	of,μg
R13612	T17599	T17600	amod	total,RNA
R13613	T17600	T17598	pobj	RNA,of
R13614	T17601	T17602	auxpass	was,loaded
R13615	T17602	T17602	ROOT	loaded,loaded
R13616	T17603	T17602	prep	per,loaded
R13617	T17604	T17603	pobj	lane,per
R13618	T17605	T17602	cc	and,loaded
R13619	T17606	T17602	conj	transferred,loaded
R13620	T17607	T17609	aux	to,charged
R13621	T17608	T17609	advmod	positively,charged
R13622	T17609	T17606	xcomp	charged,transferred
R13623	T17610	T17611	compound	nylon,membranes
R13624	T17611	T17609	dobj	membranes,charged
R13625	T17612	T17611	punct	(,membranes
R13626	T17613	T17614	amod	Hybond-N,+
R13627	T17614	T17611	appos	+,membranes
R13628	T17615	T17611	punct	;,membranes
R13629	T17616	T17617	compound	GE,Healthcare
R13630	T17617	T17611	appos	Healthcare,membranes
R13631	T17618	T17611	punct	),membranes
R13632	T17619	T17611	punct	",",membranes
R13633	T17620	T17622	nsubjpass	which,confirmed
R13634	T17621	T17622	auxpass	was,confirmed
R13714	T17701	T17704	nummod	CD8,cells

UBERON-AE

Id	Subject	Object	Predicate	Lexical cue
T14353	1279-1285	http://purl.obolibrary.org/obo/UBERON_0002106	denotes	spleen
T14354	1290-1295	http://purl.obolibrary.org/obo/UBERON_0002391	denotes	lymph
T14355	1290-1300	http://purl.obolibrary.org/obo/UBERON_0000029	denotes	lymph node

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T13819	737-746	http://purl.obolibrary.org/obo/GO_0006915	denotes	Apoptosis
T13820	737-746	http://purl.obolibrary.org/obo/GO_0097194	denotes	Apoptosis
T14374	1123-1126	http://purl.obolibrary.org/obo/GO_0006283	denotes	TCR
T14375	1886-1889	http://purl.obolibrary.org/obo/GO_0006283	denotes	TCR
T16376	4475-4494	http://purl.obolibrary.org/obo/GO_0001816	denotes	cytokine production
T16377	4475-4501	http://purl.obolibrary.org/obo/GO_0002369	denotes	cytokine production, cells
T16378	4475-4501	http://purl.obolibrary.org/obo/GO_0002370	denotes	cytokine production, cells
T16379	4475-4501	http://purl.obolibrary.org/obo/GO_0002371	denotes	cytokine production, cells
T16380	4475-4501	http://purl.obolibrary.org/obo/GO_0032762	denotes	cytokine production, cells
T16381	4475-4501	http://purl.obolibrary.org/obo/GO_0002368	denotes	cytokine production, cells
T16382	4475-4501	http://purl.obolibrary.org/obo/GO_0035744	denotes	cytokine production, cells
T16383	4475-4501	http://purl.obolibrary.org/obo/GO_0035745	denotes	cytokine production, cells
T16384	4967-4969	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T16652	5087-5099	http://purl.obolibrary.org/obo/GO_0009293	denotes	transduction
T16653	5129-5141	http://purl.obolibrary.org/obo/GO_0009293	denotes	transduction
T16654	5325-5328	http://purl.obolibrary.org/obo/GO_0006283	denotes	TCR
T16655	5577-5579	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T16944	6056-6058	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T16945	6412-6417	http://purl.obolibrary.org/obo/GO_0019835	denotes	lysis
T16946	6523-6534	http://purl.obolibrary.org/obo/GO_0016791	denotes	phosphatase
T16947	7461-7471	http://purl.obolibrary.org/obo/GO_0004175	denotes	proteinase
T17562	9215-9220	http://purl.obolibrary.org/obo/GO_0019835	denotes	lysis
T15053	2690-2693	http://purl.obolibrary.org/obo/GO_0006283	denotes	TCR
T15054	2781-2784	http://purl.obolibrary.org/obo/GO_0006283	denotes	TCR
T15055	2988-3003	http://purl.obolibrary.org/obo/GO_0001775	denotes	activated cells
T15056	3018-3028	http://purl.obolibrary.org/obo/GO_0065007	denotes	regulation

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T16634	4849-4859	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T16635	4967-4969	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T14220	62-72	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T14221	416-426	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T17518	6056-6058	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T17519	6523-6534	http://purl.obolibrary.org/obo/GO_0016791	denotes	phosphatase
T17520	6785-6793	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T17521	7461-7471	http://purl.obolibrary.org/obo/GO_0004175	denotes	proteinase
T14222	559-567	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T14223	151-155	http://purl.obolibrary.org/obo/GO_0005134	denotes	IL-2
T15774	2751-2755	http://purl.obolibrary.org/obo/GO_0005134	denotes	IL-2
T15775	3144-3148	http://purl.obolibrary.org/obo/GO_0005134	denotes	IL-2
T16913	5577-5579	http://purl.obolibrary.org/obo/GO_0003964	denotes	RT
T18085	8640-8653	http://purl.obolibrary.org/obo/GO_0003735	denotes	ribosomal RNA
T18086	9205-9209	http://purl.obolibrary.org/obo/GO_0005134	denotes	IL-2
T18087	9461-9471	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T14230	82-95	http://purl.obolibrary.org/obo/GO_0005622	denotes	intracellular
T14231	861-865	http://purl.obolibrary.org/obo/GO_0019013	denotes	Core
T14232	966-970	http://purl.obolibrary.org/obo/GO_0019013	denotes	Core
T14827	1073-1078	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14828	1087-1092	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14829	1301-1306	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14830	1315-1320	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14831	1656-1661	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14832	1683-1688	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14833	1858-1863	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14834	1947-1952	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14835	2019-2024	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14836	2064-2069	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14837	1123-1126	http://purl.obolibrary.org/obo/GO_0042101	denotes	TCR
T14838	1886-1889	http://purl.obolibrary.org/obo/GO_0042101	denotes	TCR
T15776	2158-2163	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15777	2202-2207	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15778	2333-2338	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15779	2390-2395	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15780	2518-2523	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15781	2584-2589	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15782	2605-2610	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15783	2826-2831	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15784	2928-2933	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15785	2998-3003	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15786	3125-3130	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15787	3277-3282	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15788	3327-3332	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15789	3394-3399	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15790	3671-3676	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15791	3798-3803	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15792	3870-3875	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T15793	2690-2693	http://purl.obolibrary.org/obo/GO_0042101	denotes	TCR
T15794	2781-2784	http://purl.obolibrary.org/obo/GO_0042101	denotes	TCR
T15795	2941-2953	http://purl.obolibrary.org/obo/GO_0009986	denotes	cell-surface
T16332	3953-3958	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16333	4049-4054	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16334	4176-4181	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16335	4324-4329	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16336	4404-4409	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16914	5118-5123	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16915	5234-5239	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16916	5354-5359	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16917	5325-5328	http://purl.obolibrary.org/obo/GO_0042101	denotes	TCR
T18088	8550-8559	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T18089	8669-8677	http://purl.obolibrary.org/obo/GO_0016020	denotes	membrane
T18090	8679-8688	http://purl.obolibrary.org/obo/GO_0016020	denotes	Membranes
T18091	8640-8653	http://purl.obolibrary.org/obo/GO_0005840	denotes	ribosomal RNA
T18092	9072-9076	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T18093	9119-9124	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18094	9339-9344	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18095	9461-9471	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T18096	9461-9471	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T14224	62-72	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T14225	416-426	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T14226	559-567	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T16636	4496-4501	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16637	4727-4732	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16638	4609-4622	http://purl.obolibrary.org/obo/GO_0005622	denotes	intracellular
T16639	4849-4859	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T16640	4849-4859	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T17522	5829-5834	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T17523	6125-6130	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T17524	6395-6400	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T17525	6816-6820	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T17526	8300-8304	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T17527	6552-6561	http://purl.obolibrary.org/obo/GO_0000785	denotes	chromatin
T17528	6654-6663	http://purl.obolibrary.org/obo/GO_0000785	denotes	chromatin
T17529	6720-6729	http://purl.obolibrary.org/obo/GO_0000785	denotes	chromatin
T17530	7362-7371	http://purl.obolibrary.org/obo/GO_0000785	denotes	chromatin
T17531	8246-8255	http://purl.obolibrary.org/obo/GO_0000785	denotes	chromatin
T17532	6785-6793	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T17533	6785-6793	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T14227	62-72	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T14228	416-426	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T14229	559-567	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T18254	9832-9837	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18255	9919-9924	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells

sentences

Id	Subject	Object	Predicate	Lexical cue
T13821	23-47	Sentence	denotes	Antibodies and reagents.
T13822	48-231	Sentence	denotes	The following antibodies used for intracellular or surface stains were obtained from eBioscience: anti–IL-2, anti–IFN-γ, anti-TNF, anti–granzyme B, anti-CD8, anti-CD25, and anti-CD44.
T13823	232-264	Sentence	denotes	Anti-CD69 was purchased from BD.
T13824	265-401	Sentence	denotes	For ChIP experiments, the anti-Eomes antibody was obtained from Abcam and the anti-Runx3 antibody was produced by the Groner laboratory.
T13825	402-548	Sentence	denotes	The following antibodies were used for immunoblotting: antiperforin (Abcam), anti-Eomes (Abcam), and anti–Pol-II (Santa Cruz Biotechnology, Inc.).
T13826	549-642	Sentence	denotes	The T-bet antibody was provided by L. Glimcher (Harvard School of Public Health, Boston, MA).
T13827	643-721	Sentence	denotes	The following reagents were used for the experiments presented in this report:
T13828	722-896	Sentence	denotes	Annexin V–FITC Apoptosis Detection Kit (BD), CD8 Negative Isolation Kit (Invitrogen), CD8 MicroBeads (Miltenyi Biotec), and SYBR Green PCR Core Reagents (Applied Biosystems).
T13829	897-1031	Sentence	denotes	The Gp33 peptide (KAVYNFATC) was synthesized by the Tufts University Core Facility, and 10 mM of stock solutions was prepared in DMSO.
T15043	2138-2183	Sentence	denotes	Isolation of CD8+ T cells from Runx3−/− mice.
T15044	2184-2267	Sentence	denotes	Runx3-deficient T cells fail to silence CD4 expression normally (Fig. S1) (12, 13).
T15045	2268-2440	Sentence	denotes	We therefore further fractionated the positively selected CD8+ T cells from Runx3 KO mice into CD8+CD4− SP or CD8+CD4+ DP cells by separation using anti-CD4 magnetic beads.
T15046	2441-2600	Sentence	denotes	This yielded a Runx3 KO SP “enriched” population that contained 75% CD8+CD4− cells and a KO DP enriched population that contained 85% CD8+CD4+ cells (Fig. S1).
T15047	2601-2756	Sentence	denotes	The cells were stimulated with anti-CD3+ anti-CD28 for 2 d before removing them from the TCR stimulus and culturing them in media containing 100 U/ml IL-2.
T15048	2757-2905	Sentence	denotes	As previously reported, TCR-induced proliferation of Runx3−/− CD8+ T cells was severely impaired, irrespective of CD4 expression (Fig. S1) (12, 13).
T15049	2906-3056	Sentence	denotes	However, the Runx3−/− cells showed cell-surface expression patterns indicative of activated cells, including up-regulation of CD25 and CD69 (Fig. S1).
T15050	3057-3293	Sentence	denotes	As expected from their ability to up-regulate CD25, Runx3−/− CD8+ T cells responded to IL-2 supplementation after day 2 and efficiently expanded until day 6 of the culture period, albeit at slower rates compared with WT cells (Fig. S1).
T15051	3294-3746	Sentence	denotes	Although a fraction of the KO DP cells silenced CD4 expression after activation, the ratio of SP/DP cells in each enriched population remained constant thereafter, and we did not observe any major differences between these two populations throughout the culture period, indicating that in terms of effector CTL differentiation and under our culture conditions, Runx3−/− CD8+ T cells that also coexpress CD4 are indistinguishable from those that do not.
T15052	3747-3876	Sentence	denotes	The data presented in Fig. S2 are from Runx3 KO SP cells, whereas those shown in Figs. 3 and 4 are from total Runx3 KO CD8 cells.
T16126	3878-3908	Sentence	denotes	FACS-based cytotoxicity assay.
T16127	3909-4144	Sentence	denotes	To measure cytotoxicity, EL4 thymoma target cells were loaded with 0 or 1 μM Gp33 peptide for 2 h before a 2-h coincubation with P14 CD8+ T cells at the effector-to-target ratios indicated in the figures in 96-well round-bottom plates.
T16128	4145-4244	Sentence	denotes	After the coincubation period, cells were stained with Annexin V–FITC and anti-CD8–allophycocyanin.
T16129	4245-4425	Sentence	denotes	Data analysis was performed with FlowJo software (Tree Star, Inc.); EL4 target cells (CD8-negative events) were gated, and the percentage of Annexin V+ target cells was determined.
T16373	4427-4464	Sentence	denotes	Cytokine and surface marker staining.
T16374	4465-4683	Sentence	denotes	To assess cytokine production, cells were restimulated with 10 nM PMA + 1 μM ionomycin for 6 h (unless indicated otherwise in the figures), and intracellular cytokine stains were performed as previously described (28).
T16375	4684-5074	Sentence	denotes	To detect expression of surface molecules, cells were washed in PBS, resuspended in FACS wash buffer (3% FBS, 0.1% sodium azide, 30 mM Hepes, 1× PBS) containing the antibodies indicated in the figures at previously optimized concentrations, incubated for 15 min at room temperature (RT), washed, and resuspended in 2% formaldehyde fixative solution before acquisition on a FACSCalibur (BD).
T16647	5076-5124	Sentence	denotes	Retroviral transduction of primary CD8+ T cells.
T16648	5125-5297	Sentence	denotes	For transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g.
T16649	5298-5528	Sentence	denotes	At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus.
T16650	5529-5627	Sentence	denotes	The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h.
T16651	5628-5778	Sentence	denotes	Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8).
T16948	5780-5812	Sentence	denotes	ChIP and real-time PCR analysis.
T16949	5813-6059	Sentence	denotes	20 × 106 CD8+ T cells per immunoprecipitation were fixed by adding a 1/10th volume of fixation solution (11.1% formaldehyde, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 50 mM Hepes) to 1 volume of culture media and were incubated for 10 or 30 min at RT.
T16950	6060-6118	Sentence	denotes	Fixation was stopped with 120 mM glycine on ice for 5 min.
T16951	6119-6338	Sentence	denotes	Fixed cells were washed 2× with cold PBS, 1× with cold solution I (10 mM Tris [pH 7.5], 10 mM EDTA, 0.5 mM EGTA, 1% Triton X-100), and 1× with cold solution II (10 mM Tris [pH 7.5], 1 mM EDTA, 0.5 mM EGTA, 200 mM NaCl).
T16952	6339-6623	Sentence	denotes	After washes, cell pellets were resuspended at 40 × 106 cells/ml in ChIP lysis buffer (150 mM NaCl, 25 mM Tris [pH 7.5], 1% Triton X-100, 0.1% SDS, 0.5% deoxycholate plus protease and phosphatase inhibitors), and chromatin was sheared with a sonicator to yield 0.5–1-kb DNA fragments.
T16953	6624-6833	Sentence	denotes	After preclearing the sheared chromatin with protein A–sepharose beads and removing 5% as input chromatin, immunoprecipitation was performed by adding optimized antibody amounts (per 20 × 106 cell equivalents:
T16954	6834-7001	Sentence	denotes	2.5 μg anti-Eomes, 1:100 dilution anti-Runx3), followed by overnight incubation at 4°C; protein A–sepharose beads were added for the last 3 h of the incubation period.
T16955	7002-7236	Sentence	denotes	Beads were washed 2× with RIPA buffer (50 mM Tris [pH 8], 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% deoxycholate), 1× with high salt buffer (50 mM Tris [pH 8], 500 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS), and 1× with TE buffer.
T16956	7237-7341	Sentence	denotes	After the last wash, DNA was eluted by resuspending the beads in elution buffer (1% SDS, 100 mM NaHCO3).
T16957	7342-7547	Sentence	denotes	Both input and ChIP chromatin were then treated with RNase A (5 μg total) for 1 h at 37°C, followed by the addition of proteinase K (100 μg total) and overnight incubation at 65°C to reverse cross-linking.
T16958	7548-7700	Sentence	denotes	DNA was then purified with QIAquick columns (Gel Extraction Kit; QIAGEN) according to the manufacturer's instructions and resuspended in a 50-μl volume.
T16959	7701-7941	Sentence	denotes	For real-time PCR detection of immunoprecipitated targets using the SYBR Green PCR Kit, a standard curve was obtained with serial dilutions of input DNA for each sample, and 1 μl ChIP DNA was used per PCR reaction (performed in duplicates).
T16960	7942-8038	Sentence	denotes	Melt curves and agarose gels were analyzed to ensure amplification of specific target sequences.
T16961	8039-8151	Sentence	denotes	Refer to Table S1 (available at http://www.jem.org/cgi/content/full/jem.20081242/DC1) for a list of primer sets.
T16962	8152-8334	Sentence	denotes	The data are presented as the number of immunoprecipitated target sequences relative to input chromatin, assuming two copies of target sequence per cell equivalent used for the ChIP.
T17563	8336-8371	Sentence	denotes	Northern and Western blot analyses.
T17564	8372-8456	Sentence	denotes	RNA isolation and Northern blot analysis was performed as previously described (29).
T17565	8457-8678	Sentence	denotes	In brief, 10 μg of total RNA was loaded per lane and transferred to positively charged nylon membranes (Hybond-N+; GE Healthcare), which was confirmed by ethidium bromide staining of ribosomal RNA species on the membrane.
T17566	8679-8843	Sentence	denotes	Membranes were hybridized with 1 ng/ml α-[32P]dCTP–labeled trichloroacetic acid precipitable probe in ExpressHyb hybridization buffer (Clontech Laboratories, Inc.).
T17567	8844-8983	Sentence	denotes	All cDNA probes were confirmed to have the appropriate single-copy specificity under these conditions using genomic Southern blot analysis.
T17568	8984-9043	Sentence	denotes	Band intensities were acquired by phosphorimaging analysis.
T17569	9044-9417	Sentence	denotes	For Western analysis, whole-cell protein lysates were obtained from CD8+ T cells at the time points indicated in the figures during clonal expansion in 100 U/ml IL-2 with lysis buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 10% glycerol, 5 mM EDTA, 1% NP-40) by resuspending samples in 10 μl per 106 cells and incubating on ice for 30 min in the presence of protease inhibitors.
T17570	9418-9560	Sentence	denotes	Immunoblot analysis was performed with the antibodies indicated in the figures after SDS-PAGE (10–30 μg of total protein was loaded per well).
T17571	9561-9744	Sentence	denotes	Quantification of detected protein was performed with an Intelligent Dark Box unit (LAS-3000; Fujifilm) and normalized for loading with the amount of RNA Pol-II detected in each lane.
T18121	9746-9775	Sentence	denotes	Online supplemental material.
T18122	9776-9857	Sentence	denotes	Fig. S1 shows the characterization of peripheral CD8+ T cells from Runx3−/− mice.
T18123	9858-9954	Sentence	denotes	Fig. S2 shows effector protein expression by Runx3 WT and KO cells at day 4 of in vitro culture.
T18124	9955-10020	Sentence	denotes	Primer sequences used for ChIP experiments are shown in Table S1.
T18125	10021-10119	Sentence	denotes	Online supplemental material is available at http://www.jem.org/cgi/content/full/jem.20081242/DC1.
T14376	1033-1079	Sentence	denotes	Isolation and culture of primary CD8+ T cells.
T14377	1080-1307	Sentence	denotes	CD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (>95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells.
T14378	1308-1416	Sentence	denotes	CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec).
T14379	1417-1845	Sentence	denotes	All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG.
T14380	1846-1999	Sentence	denotes	After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2.
T14381	2000-2136	Sentence	denotes	Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2.
T146	0-21	Sentence	denotes	MATERIALS AND METHODS
T147	23-47	Sentence	denotes	Antibodies and reagents.
T148	48-231	Sentence	denotes	The following antibodies used for intracellular or surface stains were obtained from eBioscience: anti–IL-2, anti–IFN-γ, anti-TNF, anti–granzyme B, anti-CD8, anti-CD25, and anti-CD44.
T149	232-264	Sentence	denotes	Anti-CD69 was purchased from BD.
T150	265-401	Sentence	denotes	For ChIP experiments, the anti-Eomes antibody was obtained from Abcam and the anti-Runx3 antibody was produced by the Groner laboratory.
T151	402-548	Sentence	denotes	The following antibodies were used for immunoblotting: antiperforin (Abcam), anti-Eomes (Abcam), and anti–Pol-II (Santa Cruz Biotechnology, Inc.).
T152	549-586	Sentence	denotes	The T-bet antibody was provided by L.
T153	587-642	Sentence	denotes	Glimcher (Harvard School of Public Health, Boston, MA).
T154	643-721	Sentence	denotes	The following reagents were used for the experiments presented in this report:
T155	722-896	Sentence	denotes	Annexin V–FITC Apoptosis Detection Kit (BD), CD8 Negative Isolation Kit (Invitrogen), CD8 MicroBeads (Miltenyi Biotec), and SYBR Green PCR Core Reagents (Applied Biosystems).
T156	897-1031	Sentence	denotes	The Gp33 peptide (KAVYNFATC) was synthesized by the Tufts University Core Facility, and 10 mM of stock solutions was prepared in DMSO.
T157	1033-1079	Sentence	denotes	Isolation and culture of primary CD8+ T cells.
T158	1080-1307	Sentence	denotes	CD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (>95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells.
T159	1308-1416	Sentence	denotes	CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec).
T160	1417-1622	Sentence	denotes	All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees.
T161	1623-1845	Sentence	denotes	For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG.
T162	1846-1999	Sentence	denotes	After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2.
T163	2000-2136	Sentence	denotes	Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2.
T164	2138-2183	Sentence	denotes	Isolation of CD8+ T cells from Runx3−/− mice.
T165	2184-2267	Sentence	denotes	Runx3-deficient T cells fail to silence CD4 expression normally (Fig. S1) (12, 13).
T166	2268-2440	Sentence	denotes	We therefore further fractionated the positively selected CD8+ T cells from Runx3 KO mice into CD8+CD4− SP or CD8+CD4+ DP cells by separation using anti-CD4 magnetic beads.
T167	2441-2600	Sentence	denotes	This yielded a Runx3 KO SP “enriched” population that contained 75% CD8+CD4− cells and a KO DP enriched population that contained 85% CD8+CD4+ cells (Fig. S1).
T168	2601-2756	Sentence	denotes	The cells were stimulated with anti-CD3+ anti-CD28 for 2 d before removing them from the TCR stimulus and culturing them in media containing 100 U/ml IL-2.
T169	2757-2905	Sentence	denotes	As previously reported, TCR-induced proliferation of Runx3−/− CD8+ T cells was severely impaired, irrespective of CD4 expression (Fig. S1) (12, 13).
T170	2906-3056	Sentence	denotes	However, the Runx3−/− cells showed cell-surface expression patterns indicative of activated cells, including up-regulation of CD25 and CD69 (Fig. S1).
T171	3057-3293	Sentence	denotes	As expected from their ability to up-regulate CD25, Runx3−/− CD8+ T cells responded to IL-2 supplementation after day 2 and efficiently expanded until day 6 of the culture period, albeit at slower rates compared with WT cells (Fig. S1).
T172	3294-3746	Sentence	denotes	Although a fraction of the KO DP cells silenced CD4 expression after activation, the ratio of SP/DP cells in each enriched population remained constant thereafter, and we did not observe any major differences between these two populations throughout the culture period, indicating that in terms of effector CTL differentiation and under our culture conditions, Runx3−/− CD8+ T cells that also coexpress CD4 are indistinguishable from those that do not.
T173	3747-3833	Sentence	denotes	The data presented in Fig. S2 are from Runx3 KO SP cells, whereas those shown in Figs.
T174	3834-3876	Sentence	denotes	3 and 4 are from total Runx3 KO CD8 cells.
T175	3878-3908	Sentence	denotes	FACS-based cytotoxicity assay.
T176	3909-4144	Sentence	denotes	To measure cytotoxicity, EL4 thymoma target cells were loaded with 0 or 1 μM Gp33 peptide for 2 h before a 2-h coincubation with P14 CD8+ T cells at the effector-to-target ratios indicated in the figures in 96-well round-bottom plates.
T177	4145-4244	Sentence	denotes	After the coincubation period, cells were stained with Annexin V–FITC and anti-CD8–allophycocyanin.
T178	4245-4425	Sentence	denotes	Data analysis was performed with FlowJo software (Tree Star, Inc.); EL4 target cells (CD8-negative events) were gated, and the percentage of Annexin V+ target cells was determined.
T179	4427-4464	Sentence	denotes	Cytokine and surface marker staining.
T180	4465-4683	Sentence	denotes	To assess cytokine production, cells were restimulated with 10 nM PMA + 1 μM ionomycin for 6 h (unless indicated otherwise in the figures), and intracellular cytokine stains were performed as previously described (28).
T181	4684-5074	Sentence	denotes	To detect expression of surface molecules, cells were washed in PBS, resuspended in FACS wash buffer (3% FBS, 0.1% sodium azide, 30 mM Hepes, 1× PBS) containing the antibodies indicated in the figures at previously optimized concentrations, incubated for 15 min at room temperature (RT), washed, and resuspended in 2% formaldehyde fixative solution before acquisition on a FACSCalibur (BD).
T182	5076-5124	Sentence	denotes	Retroviral transduction of primary CD8+ T cells.
T183	5125-5297	Sentence	denotes	For transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g.
T184	5298-5528	Sentence	denotes	At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus.
T185	5529-5627	Sentence	denotes	The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h.
T186	5628-5719	Sentence	denotes	Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L.
T187	5720-5778	Sentence	denotes	Reiner (University of Pennsylvania, Philadelphia, PA) (8).
T188	5780-5812	Sentence	denotes	ChIP and real-time PCR analysis.
T189	5813-6059	Sentence	denotes	20 × 106 CD8+ T cells per immunoprecipitation were fixed by adding a 1/10th volume of fixation solution (11.1% formaldehyde, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 50 mM Hepes) to 1 volume of culture media and were incubated for 10 or 30 min at RT.
T190	6060-6118	Sentence	denotes	Fixation was stopped with 120 mM glycine on ice for 5 min.
T191	6119-6338	Sentence	denotes	Fixed cells were washed 2× with cold PBS, 1× with cold solution I (10 mM Tris [pH 7.5], 10 mM EDTA, 0.5 mM EGTA, 1% Triton X-100), and 1× with cold solution II (10 mM Tris [pH 7.5], 1 mM EDTA, 0.5 mM EGTA, 200 mM NaCl).
T192	6339-6623	Sentence	denotes	After washes, cell pellets were resuspended at 40 × 106 cells/ml in ChIP lysis buffer (150 mM NaCl, 25 mM Tris [pH 7.5], 1% Triton X-100, 0.1% SDS, 0.5% deoxycholate plus protease and phosphatase inhibitors), and chromatin was sheared with a sonicator to yield 0.5–1-kb DNA fragments.
T193	6624-6833	Sentence	denotes	After preclearing the sheared chromatin with protein A–sepharose beads and removing 5% as input chromatin, immunoprecipitation was performed by adding optimized antibody amounts (per 20 × 106 cell equivalents:
T194	6834-7001	Sentence	denotes	2.5 μg anti-Eomes, 1:100 dilution anti-Runx3), followed by overnight incubation at 4°C; protein A–sepharose beads were added for the last 3 h of the incubation period.
T195	7002-7236	Sentence	denotes	Beads were washed 2× with RIPA buffer (50 mM Tris [pH 8], 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% deoxycholate), 1× with high salt buffer (50 mM Tris [pH 8], 500 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS), and 1× with TE buffer.
T196	7237-7341	Sentence	denotes	After the last wash, DNA was eluted by resuspending the beads in elution buffer (1% SDS, 100 mM NaHCO3).
T197	7342-7547	Sentence	denotes	Both input and ChIP chromatin were then treated with RNase A (5 μg total) for 1 h at 37°C, followed by the addition of proteinase K (100 μg total) and overnight incubation at 65°C to reverse cross-linking.
T198	7548-7700	Sentence	denotes	DNA was then purified with QIAquick columns (Gel Extraction Kit; QIAGEN) according to the manufacturer's instructions and resuspended in a 50-μl volume.
T199	7701-7941	Sentence	denotes	For real-time PCR detection of immunoprecipitated targets using the SYBR Green PCR Kit, a standard curve was obtained with serial dilutions of input DNA for each sample, and 1 μl ChIP DNA was used per PCR reaction (performed in duplicates).
T200	7942-8038	Sentence	denotes	Melt curves and agarose gels were analyzed to ensure amplification of specific target sequences.
T201	8039-8151	Sentence	denotes	Refer to Table S1 (available at http://www.jem.org/cgi/content/full/jem.20081242/DC1) for a list of primer sets.
T202	8152-8334	Sentence	denotes	The data are presented as the number of immunoprecipitated target sequences relative to input chromatin, assuming two copies of target sequence per cell equivalent used for the ChIP.
T203	8336-8371	Sentence	denotes	Northern and Western blot analyses.
T204	8372-8456	Sentence	denotes	RNA isolation and Northern blot analysis was performed as previously described (29).
T205	8457-8678	Sentence	denotes	In brief, 10 μg of total RNA was loaded per lane and transferred to positively charged nylon membranes (Hybond-N+; GE Healthcare), which was confirmed by ethidium bromide staining of ribosomal RNA species on the membrane.
T206	8679-8843	Sentence	denotes	Membranes were hybridized with 1 ng/ml α-[32P]dCTP–labeled trichloroacetic acid precipitable probe in ExpressHyb hybridization buffer (Clontech Laboratories, Inc.).
T207	8844-8983	Sentence	denotes	All cDNA probes were confirmed to have the appropriate single-copy specificity under these conditions using genomic Southern blot analysis.
T208	8984-9043	Sentence	denotes	Band intensities were acquired by phosphorimaging analysis.
T209	9044-9417	Sentence	denotes	For Western analysis, whole-cell protein lysates were obtained from CD8+ T cells at the time points indicated in the figures during clonal expansion in 100 U/ml IL-2 with lysis buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 10% glycerol, 5 mM EDTA, 1% NP-40) by resuspending samples in 10 μl per 106 cells and incubating on ice for 30 min in the presence of protease inhibitors.
T210	9418-9560	Sentence	denotes	Immunoblot analysis was performed with the antibodies indicated in the figures after SDS-PAGE (10–30 μg of total protein was loaded per well).
T211	9561-9744	Sentence	denotes	Quantification of detected protein was performed with an Intelligent Dark Box unit (LAS-3000; Fujifilm) and normalized for loading with the amount of RNA Pol-II detected in each lane.
T212	9746-9775	Sentence	denotes	Online supplemental material.
T213	9776-9857	Sentence	denotes	Fig. S1 shows the characterization of peripheral CD8+ T cells from Runx3−/− mice.
T214	9858-9954	Sentence	denotes	Fig. S2 shows effector protein expression by Runx3 WT and KO cells at day 4 of in vitro culture.
T215	9955-10020	Sentence	denotes	Primer sequences used for ChIP experiments are shown in Table S1.
T216	10021-10119	Sentence	denotes	Online supplemental material is available at http://www.jem.org/cgi/content/full/jem.20081242/DC1.

events-check-again

Id	Subject	Object	Predicate	Lexical cue	Negation
T14314	151-155	Protein	denotes	IL-2
T14315	162-167	Protein	denotes	IFN-γ
T14316	174-177	Protein	denotes	TNF
T14317	184-194	Protein	denotes	granzyme B
T14318	201-204	Protein	denotes	CD8
T14319	211-215	Protein	denotes	CD25
T14320	226-230	Protein	denotes	CD44
T14321	237-241	Protein	denotes	CD69
T14322	296-301	Protein	denotes	Eomes
T14323	348-353	Protein	denotes	Runx3
T14324	461-469	Protein	denotes	perforin
T14325	484-489	Protein	denotes	Eomes
T14326	553-558	Protein	denotes	T-bet
T14327	722-731	Protein	denotes	Annexin V
T14328	767-770	Protein	denotes	CD8
T14329	808-811	Protein	denotes	CD8
T14884	1080-1083	Protein	denotes	CD8
T14885	1109-1113	Protein	denotes	Tcra
T14886	1165-1170	Protein	denotes	Tbx21
T14887	1308-1311	Protein	denotes	CD8
T14888	1326-1331	Protein	denotes	Runx3
T14889	1649-1652	Protein	denotes	CD8
T14890	1747-1750	Protein	denotes	CD3
T16026	2456-2461	Protein	denotes	Runx3
T16027	2509-2512	Protein	denotes	CD8
T16028	2513-2516	Protein	denotes	CD4
T16029	2575-2578	Protein	denotes	CD8
T16030	2579-2582	Protein	denotes	CD4
T16031	2637-2640	Protein	denotes	CD3
T16032	2647-2651	Protein	denotes	CD28
T16033	2751-2755	Protein	denotes	IL-2
T16034	2810-2815	Protein	denotes	Runx3
T16035	2819-2822	Protein	denotes	CD8
T16036	2871-2874	Protein	denotes	CD4
T16037	2875-2885	Gene_expression	denotes	expression
T16038	2919-2924	Protein	denotes	Runx3
T16039	3015-3028	Positive_regulation	denotes	up-regulation
T16040	3015-3028	Positive_regulation	denotes	up-regulation
T16041	3032-3036	Protein	denotes	CD25
T16042	3041-3045	Protein	denotes	CD69
T16043	3091-3102	Positive_regulation	denotes	up-regulate
T16044	3103-3107	Protein	denotes	CD25
T16045	3109-3114	Protein	denotes	Runx3
T16046	3118-3121	Protein	denotes	CD8
T16047	3144-3148	Protein	denotes	IL-2
T16048	3333-3341	Negative_regulation	denotes	silenced
T16049	3342-3345	Protein	denotes	CD4
T16050	3346-3356	Gene_expression	denotes	expression
T16051	3655-3660	Protein	denotes	Runx3
T16052	3664-3667	Protein	denotes	CD8
T16053	3687-3696	Gene_expression	denotes	coexpress
T16054	3697-3700	Protein	denotes	CD4
T16055	3786-3791	Protein	denotes	Runx3
T16056	3857-3862	Protein	denotes	Runx3
T16057	3866-3869	Protein	denotes	CD8
T17549	5822-5825	Protein	denotes	CD8
T17550	6846-6851	Protein	denotes	Eomes
T17551	6873-6878	Protein	denotes	Runx3
T16013	2169-2174	Protein	denotes	Runx3
T16014	2184-2189	Protein	denotes	Runx3
T16015	2190-2199	Negative_regulation	denotes	deficient
T16016	2216-2223	Negative_regulation	denotes	silence	true
T16017	2224-2227	Protein	denotes	CD4
T16018	2228-2238	Gene_expression	denotes	expression
T16019	2326-2329	Protein	denotes	CD8
T16020	2344-2349	Protein	denotes	Runx3
T16021	2363-2366	Protein	denotes	CD8
T16022	2367-2370	Protein	denotes	CD4
T16023	2378-2381	Protein	denotes	CD8
T16024	2382-2385	Protein	denotes	CD4
T16025	2421-2424	Protein	denotes	CD4
T16929	5654-5664	Protein	denotes	Eomes-VP16
T18109	9112-9115	Protein	denotes	CD8
T18110	9205-9209	Protein	denotes	IL-2
T14891	1773-1777	Protein	denotes	CD28
T14892	2129-2135	Protein	denotes	rhIL-2
T16362	4042-4045	Protein	denotes	CD8
T16363	4200-4209	Protein	denotes	Annexin V
T16364	4224-4227	Protein	denotes	CD8
T16365	4331-4334	Protein	denotes	CD8
T16366	4386-4395	Protein	denotes	Annexin V
T16928	5347-5350	Protein	denotes	CD8
T18271	9825-9828	Protein	denotes	CD8
T18272	9843-9848	Protein	denotes	Runx3
T18273	9903-9908	Protein	denotes	Runx3
R12328	T16014	T16015	themeOf	Runx3,deficient
R12329	T16015	T16016	causeOf	deficient,silence
R12330	T16017	T16018	themeOf	CD4,expression
R12331	T16018	T16016	themeOf	expression,silence
R12332	T16036	T16037	themeOf	CD4,expression
R12333	T16041	T16039	themeOf	CD25,up-regulation
R12334	T16042	T16040	themeOf	CD69,up-regulation
R12335	T16044	T16043	themeOf	CD25,up-regulate
R12336	T16049	T16050	themeOf	CD4,expression
R12337	T16050	T16048	themeOf	expression,silenced
R12338	T16054	T16053	themeOf	CD4,coexpress

bionlp-st-ge-2016-reference-tees

Id	Subject	Object	Predicate	Lexical cue
T14330	146-155	Protein	denotes	anti–IL-2
T14331	157-167	Protein	denotes	anti–IFN-γ
T14332	169-177	Protein	denotes	anti-TNF
T14333	179-194	Protein	denotes	anti–granzyme B
T14334	196-204	Protein	denotes	anti-CD8
T14335	206-215	Protein	denotes	anti-CD25
T14336	226-230	Protein	denotes	CD44
T14337	232-241	Protein	denotes	Anti-CD69
T14338	343-362	Protein	denotes	anti-Runx3 antibody
T14339	367-375	Gene_expression	denotes	produced
T14340	553-567	Protein	denotes	T-bet antibody
T14341	767-770	Protein	denotes	CD8
T14342	808-811	Protein	denotes	CD8
T14343	901-905	Protein	denotes	Gp33
T14893	1066-1070	Protein	denotes	CD8+
T14894	1080-1083	Protein	denotes	CD8
T14895	1119-1126	Protein	denotes	P14 TCR
T14896	1165-1170	Protein	denotes	Tbx21
T14897	1308-1312	Protein	denotes	CD8+
T14898	1326-1331	Protein	denotes	Runx3
T14899	1535-1549	Protein	denotes	Immune Disease
T14900	1649-1653	Protein	denotes	CD8+
T14901	1742-1750	Protein	denotes	anti-CD3
T14902	1752-1762	Protein	denotes	clone 2C11
T14903	1773-1777	Protein	denotes	CD28
T14904	1828-1844	Protein	denotes	anti–hamster IgG
T14905	1886-1889	Protein	denotes	TCR
T14906	2129-2135	Protein	denotes	rhIL-2
T16058	2151-2155	Protein	denotes	CD8+
T16059	2169-2174	Protein	denotes	Runx3
T16060	2184-2189	Protein	denotes	Runx3
T16061	2224-2227	Protein	denotes	CD4
T16062	2190-2199	Negative_regulation	denotes	deficient
T16063	2228-2238	Gene_expression	denotes	expression
T16064	2216-2223	Negative_regulation	denotes	silence
T16065	2326-2330	Protein	denotes	CD8+
T16066	2344-2352	Protein	denotes	Runx3 KO
T16067	2363-2366	Protein	denotes	CD8
T16068	2367-2370	Protein	denotes	CD4
T16069	2372-2374	Protein	denotes	SP
T16070	2378-2381	Protein	denotes	CD8
T16071	2382-2385	Protein	denotes	CD4
T16072	2456-2464	Protein	denotes	Runx3 KO
T16073	2509-2512	Protein	denotes	CD8
T16074	2513-2516	Protein	denotes	CD4
T16075	2575-2578	Protein	denotes	CD8
T16076	2579-2582	Protein	denotes	CD4
T16077	2632-2640	Protein	denotes	anti-CD3
T16078	2642-2651	Protein	denotes	anti-CD28
T16079	2690-2693	Protein	denotes	TCR
T16080	2781-2784	Protein	denotes	TCR
T16081	2810-2815	Protein	denotes	Runx3
T16082	2819-2822	Protein	denotes	CD8
T16083	2871-2874	Protein	denotes	CD4
T16084	2875-2885	Gene_expression	denotes	expression
T16085	2919-2924	Protein	denotes	Runx3
T16086	3032-3036	Protein	denotes	CD25
T16087	3041-3045	Protein	denotes	CD69
T16088	3047-3050	Protein	denotes	Fig
T16089	3052-3054	Protein	denotes	S1
T16090	3015-3028	Positive_regulation	denotes	up-regulation
T16091	3015-3028	Positive_regulation	denotes	up-regulation
T16092	3015-3028	Positive_regulation	denotes	up-regulation
T16093	3103-3107	Protein	denotes	CD25
T16094	3109-3114	Protein	denotes	Runx3
T16095	3118-3121	Protein	denotes	CD8
T16096	3144-3148	Protein	denotes	IL-2
T16097	3091-3102	Positive_regulation	denotes	up-regulate
T16098	3342-3345	Protein	denotes	CD4
T16099	3655-3660	Protein	denotes	Runx3
T16100	3664-3667	Protein	denotes	CD8
T16101	3697-3700	Protein	denotes	CD4
T16102	3346-3356	Gene_expression	denotes	expression
T16103	3687-3696	Gene_expression	denotes	coexpress
T16104	3333-3341	Negative_regulation	denotes	silenced
T16105	3769-3772	Protein	denotes	Fig
T16106	3756-3765	Gene_expression	denotes	presented
T16107	3774-3776	Protein	denotes	S2
T16108	3786-3794	Protein	denotes	Runx3 KO
T16109	3857-3862	Protein	denotes	Runx3
T16110	3863-3869	Protein	denotes	KO CD8
T16930	5111-5115	Protein	denotes	CD8+
T16931	5325-5328	Protein	denotes	TCR
T16932	5347-5350	Protein	denotes	CD8
T16933	5654-5659	Protein	denotes	Eomes
T16934	5660-5664	Protein	denotes	VP16
T17552	6505-6534	Protein	denotes	plus protease and phosphatase
T17553	7395-7402	Protein	denotes	RNase A
T17554	8086-8089	Protein	denotes	org
T17555	8090-8093	Protein	denotes	cgi
T18111	9112-9116	Protein	denotes	CD8+
T16367	3934-3937	Protein	denotes	EL4
T16368	3983-3998	Protein	denotes	μM Gp33 peptide
T16369	4038-4041	Protein	denotes	P14
T16370	4224-4227	Protein	denotes	CD8
T16371	4228-4243	Protein	denotes	allophycocyanin
T16372	4331-4334	Protein	denotes	CD8
T18274	9776-9779	Protein	denotes	Fig
T18275	9781-9783	Protein	denotes	S1
T18276	9825-9829	Protein	denotes	CD8+
T18277	9843-9848	Protein	denotes	Runx3
T18278	9858-9861	Protein	denotes	Fig
T18279	9903-9911	Protein	denotes	Runx3 WT
T18280	10011-10019	Protein	denotes	Table S1
T18281	10081-10084	Protein	denotes	org
T18282	10085-10088	Protein	denotes	cgi
R11154	T14338	T14339	themeOf	anti-Runx3 antibody,produced
R12339	T16060	T16062	themeOf	Runx3,deficient
R12340	T16061	T16063	themeOf	CD4,expression
R12341	T16062	T16064	causeOf	deficient,silence
R12342	T16063	T16064	themeOf	expression,silence
R12343	T16083	T16084	themeOf	CD4,expression
R12344	T16086	T16090	themeOf	CD25,up-regulation
R12345	T16087	T16091	themeOf	CD69,up-regulation
R12346	T16089	T16092	themeOf	S1,up-regulation
R12347	T16093	T16097	themeOf	CD25,up-regulate
R12348	T16098	T16102	themeOf	CD4,expression
R12349	T16101	T16103	themeOf	CD4,coexpress
R12350	T16102	T16104	themeOf	expression,silenced
R12351	T16105	T16106	themeOf	Fig,presented

bionlp-st-ge-2016-reference

Id	Subject	Object	Predicate	Lexical cue	Negation
T15042	3866-3869	Protein	denotes	CD8
T16121	4042-4045	Protein	denotes	CD8
T16122	4200-4209	Protein	denotes	Annexin V
T16123	4224-4227	Protein	denotes	CD8
T16124	4331-4334	Protein	denotes	CD8
T16125	4386-4395	Protein	denotes	Annexin V
T13803	151-155	Protein	denotes	IL-2
T13804	162-167	Protein	denotes	IFN-γ
T13805	174-177	Protein	denotes	TNF
T13806	184-194	Protein	denotes	granzyme B
T13807	201-204	Protein	denotes	CD8
T13808	211-215	Protein	denotes	CD25
T13809	226-230	Protein	denotes	CD44
T13810	237-241	Protein	denotes	CD69
T13811	296-301	Protein	denotes	Eomes
T13812	348-353	Protein	denotes	Runx3
T13813	461-469	Protein	denotes	perforin
T13814	484-489	Protein	denotes	Eomes
T13815	553-558	Protein	denotes	T-bet
T13816	722-731	Protein	denotes	Annexin V
T13817	767-770	Protein	denotes	CD8
T13818	808-811	Protein	denotes	CD8
T14365	1080-1083	Protein	denotes	CD8
T14366	1109-1113	Protein	denotes	Tcra
T14367	1165-1170	Protein	denotes	Tbx21
T14368	1308-1311	Protein	denotes	CD8
T14369	1326-1331	Protein	denotes	Runx3
T14370	1649-1652	Protein	denotes	CD8
T14371	1747-1750	Protein	denotes	CD3
T14372	1773-1777	Protein	denotes	CD28
T14373	2129-2135	Protein	denotes	rhIL-2
T16941	5822-5825	Protein	denotes	CD8
T16942	6846-6851	Protein	denotes	Eomes
T16943	6873-6878	Protein	denotes	Runx3
T17560	9112-9115	Protein	denotes	CD8
T17561	9205-9209	Protein	denotes	IL-2
T16645	5347-5350	Protein	denotes	CD8
T16646	5654-5664	Protein	denotes	Eomes-VP16
T18118	9825-9828	Protein	denotes	CD8
T18119	9843-9848	Protein	denotes	Runx3
T18120	9903-9908	Protein	denotes	Runx3
T14998	2169-2174	Protein	denotes	Runx3
T14999	2184-2189	Protein	denotes	Runx3
T15000	2190-2199	Negative_regulation	denotes	deficient
T15001	2216-2223	Negative_regulation	denotes	silence	true
T15002	2224-2227	Protein	denotes	CD4
T15003	2228-2238	Gene_expression	denotes	expression
T15004	2326-2329	Protein	denotes	CD8
T15005	2344-2349	Protein	denotes	Runx3
T15006	2363-2366	Protein	denotes	CD8
T15007	2367-2370	Protein	denotes	CD4
T15008	2378-2381	Protein	denotes	CD8
T15009	2382-2385	Protein	denotes	CD4
T15010	2421-2424	Protein	denotes	CD4
T15011	2456-2461	Protein	denotes	Runx3
T15012	2509-2512	Protein	denotes	CD8
T15013	2513-2516	Protein	denotes	CD4
T15014	2575-2578	Protein	denotes	CD8
T15015	2579-2582	Protein	denotes	CD4
T15016	2637-2640	Protein	denotes	CD3
T15017	2647-2651	Protein	denotes	CD28
T15018	2751-2755	Protein	denotes	IL-2
T15019	2810-2815	Protein	denotes	Runx3
T15020	2819-2822	Protein	denotes	CD8
T15021	2871-2874	Protein	denotes	CD4
T15022	2875-2885	Gene_expression	denotes	expression
T15023	2919-2924	Protein	denotes	Runx3
T15024	3015-3028	Positive_regulation	denotes	up-regulation
T15025	3015-3028	Positive_regulation	denotes	up-regulation
T15026	3032-3036	Protein	denotes	CD25
T15027	3041-3045	Protein	denotes	CD69
T15028	3091-3102	Positive_regulation	denotes	up-regulate
T15029	3103-3107	Protein	denotes	CD25
T15030	3109-3114	Protein	denotes	Runx3
T15031	3118-3121	Protein	denotes	CD8
T15032	3144-3148	Protein	denotes	IL-2
T15033	3333-3341	Negative_regulation	denotes	silenced
T15034	3342-3345	Protein	denotes	CD4
T15035	3346-3356	Gene_expression	denotes	expression
T15036	3655-3660	Protein	denotes	Runx3
T15037	3664-3667	Protein	denotes	CD8
T15038	3687-3696	Gene_expression	denotes	coexpress
T15039	3697-3700	Protein	denotes	CD4
T15040	3786-3791	Protein	denotes	Runx3
T15041	3857-3862	Protein	denotes	Runx3
R11608	T14999	T15000	themeOf	Runx3,deficient
R11609	T15000	T15001	causeOf	deficient,silence
R11610	T15002	T15003	themeOf	CD4,expression
R11611	T15003	T15001	themeOf	expression,silence
R11612	T15021	T15022	themeOf	CD4,expression
R11613	T15026	T15024	themeOf	CD25,up-regulation
R11614	T15027	T15025	themeOf	CD69,up-regulation
R11615	T15029	T15028	themeOf	CD25,up-regulate
R11616	T15034	T15035	themeOf	CD4,expression
R11617	T15035	T15033	themeOf	expression,silenced
R11618	T15039	T15038	themeOf	CD4,coexpress

bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T14195	151-155	P60568	denotes	IL-2
T14196	162-167	P01579	denotes	IFN-γ
T14197	174-177	P01375	denotes	TNF
T14198	184-194	P10144	denotes	granzyme B
T14199	201-204	P01732	denotes	CD8
T14200	201-204	P10966	denotes	CD8
T14201	211-215	P01589	denotes	CD25
T14202	296-301	O95936	denotes	Eomes
T14203	348-353	Q13761	denotes	Runx3
T14204	484-489	O95936	denotes	Eomes
T14205	553-558	Q9UL17	denotes	T-bet
T14206	722-731	P08758	denotes	Annexin V
T14207	767-770	P10966	denotes	CD8
T14208	767-770	P01732	denotes	CD8
T14209	808-811	P10966	denotes	CD8
T14210	808-811	P01732	denotes	CD8
T14806	1066-1069	P01732	denotes	CD8
T14807	1066-1069	P10966	denotes	CD8
T14808	1080-1083	P10966	denotes	CD8
T14809	1080-1083	P01732	denotes	CD8
T14810	1165-1170	Q9UL17	denotes	Tbx21
T14811	1308-1311	P01732	denotes	CD8
T14812	1308-1311	P10966	denotes	CD8
T14813	1326-1331	Q13761	denotes	Runx3
T14814	1649-1652	P10966	denotes	CD8
T14815	1649-1652	P01732	denotes	CD8
T14816	1747-1750	P04234	denotes	CD3
T14817	1747-1750	P20963	denotes	CD3
T14818	1747-1750	P09693	denotes	CD3
T14819	1747-1750	P07766	denotes	CD3
T14820	1773-1777	P10747	denotes	CD28
T15750	3103-3107	P01589	denotes	CD25
T15751	3109-3114	Q13761	denotes	Runx3
T15752	3118-3121	P01732	denotes	CD8
T15753	3118-3121	P10966	denotes	CD8
T15754	3144-3148	P60568	denotes	IL-2
T15755	3342-3345	P01730	denotes	CD4
T15756	3655-3660	Q13761	denotes	Runx3
T15757	3664-3667	P01732	denotes	CD8
T15758	3664-3667	P10966	denotes	CD8
T15759	3697-3700	P01730	denotes	CD4
T15760	3786-3791	Q13761	denotes	Runx3
T15761	3857-3862	Q13761	denotes	Runx3
T15762	3866-3869	P10966	denotes	CD8
T15763	3866-3869	P01732	denotes	CD8
T17493	5822-5825	P10966	denotes	CD8
T17494	5822-5825	P01732	denotes	CD8
T17495	6198-6200	P0A7Z4	denotes	pH
T17496	6292-6294	P0A7Z4	denotes	pH
T17497	6451-6453	P0A7Z4	denotes	pH
T17498	6846-6851	O95936	denotes	Eomes
T17499	6873-6878	Q13761	denotes	Runx3
T17500	7053-7055	P0A7Z4	denotes	pH
T17501	7162-7164	P0A7Z4	denotes	pH
T17502	8228-8236	Q04864	denotes	relative
T15716	2151-2154	P01732	denotes	CD8
T15717	2151-2154	P10966	denotes	CD8
T15718	2169-2174	Q13761	denotes	Runx3
T15719	2184-2189	Q13761	denotes	Runx3
T15720	2224-2227	P01730	denotes	CD4
T15721	2326-2329	P10966	denotes	CD8
T15722	2326-2329	P01732	denotes	CD8
T15723	2344-2349	Q13761	denotes	Runx3
T15724	2363-2366	P10966	denotes	CD8
T15725	2363-2366	P01732	denotes	CD8
T15726	2367-2370	P01730	denotes	CD4
T15727	2378-2381	P01732	denotes	CD8
T15728	2378-2381	P10966	denotes	CD8
T15729	2382-2385	P01730	denotes	CD4
T15730	2421-2424	P01730	denotes	CD4
T15731	2456-2461	Q13761	denotes	Runx3
T15732	2509-2512	P01732	denotes	CD8
T15733	2509-2512	P10966	denotes	CD8
T15734	2513-2516	P01730	denotes	CD4
T15735	2575-2578	P01732	denotes	CD8
T15736	2575-2578	P10966	denotes	CD8
T15737	2579-2582	P01730	denotes	CD4
T15738	2637-2640	P20963	denotes	CD3
T15739	2637-2640	P09693	denotes	CD3
T15740	2637-2640	P04234	denotes	CD3
T15741	2637-2640	P07766	denotes	CD3
T15742	2647-2651	P10747	denotes	CD28
T15743	2751-2755	P60568	denotes	IL-2
T15744	2810-2815	Q13761	denotes	Runx3
T15745	2819-2822	P10966	denotes	CD8
T15746	2819-2822	P01732	denotes	CD8
T15747	2871-2874	P01730	denotes	CD4
T15748	2919-2924	Q13761	denotes	Runx3
T15749	3032-3036	P01589	denotes	CD25
T16320	4042-4045	P10966	denotes	CD8
T16321	4042-4045	P01732	denotes	CD8
T16322	4200-4209	P08758	denotes	Annexin V
T16323	4224-4227	P10966	denotes	CD8
T16324	4224-4227	P01732	denotes	CD8
T16325	4331-4334	P01732	denotes	CD8
T16326	4331-4334	P10966	denotes	CD8
T16327	4386-4395	P08758	denotes	Annexin V
T16903	5111-5114	P10966	denotes	CD8
T16904	5111-5114	P01732	denotes	CD8
T16905	5347-5350	P10966	denotes	CD8
T16906	5347-5350	P01732	denotes	CD8
T16907	5654-5659	O95936	denotes	Eomes
T18072	9112-9115	P10966	denotes	CD8
T18073	9112-9115	P01732	denotes	CD8
T18074	9205-9209	P60568	denotes	IL-2
T18075	9241-9243	P0A7Z4	denotes	pH
T18245	9825-9828	P10966	denotes	CD8
T18246	9825-9828	P01732	denotes	CD8
T18247	9843-9848	Q13761	denotes	Runx3
T18248	9903-9908	Q13761	denotes	Runx3

test2

Id	Subject	Object	Predicate	Lexical cue	Negation
T13786	151-155	Protein	denotes	IL-2
T13787	162-167	Protein	denotes	IFN-γ
T13788	174-177	Protein	denotes	TNF
T13789	184-194	Protein	denotes	granzyme B
T13790	201-204	Protein	denotes	CD8
T13791	211-215	Protein	denotes	CD25
T13792	226-230	Protein	denotes	CD44
T13793	237-241	Protein	denotes	CD69
T13794	296-301	Protein	denotes	Eomes
T13795	348-353	Protein	denotes	Runx3
T13796	367-375	Gene_expression	denotes	produced
T13797	457-469	Protein	denotes	antiperforin
T13798	484-489	Protein	denotes	Eomes
T13799	553-558	Protein	denotes	T-bet
T13800	722-731	Protein	denotes	Annexin V
T13801	767-770	Protein	denotes	CD8
T13802	808-811	Protein	denotes	CD8
T14356	1080-1083	Protein	denotes	CD8
T14357	1109-1113	Protein	denotes	Tcra
T14358	1165-1170	Protein	denotes	Tbx21
T14359	1308-1311	Protein	denotes	CD8
T14360	1326-1331	Protein	denotes	Runx3
T14361	1649-1652	Protein	denotes	CD8
T14362	1747-1750	Protein	denotes	CD3
T14363	1773-1777	Protein	denotes	CD28
T14364	2129-2135	Protein	denotes	rhIL-2
T14952	2169-2174	Protein	denotes	Runx3
T14953	2184-2189	Protein	denotes	Runx3	true
T14954	2190-2199	Negative_regulation	denotes	deficient
T14955	2216-2223	Negative_regulation	denotes	silence
T14956	2224-2227	Protein	denotes	CD4
T14957	2228-2238	Gene_expression	denotes	expression
T14958	2326-2329	Protein	denotes	CD8
T14959	2344-2349	Protein	denotes	Runx3
T14960	2363-2366	Protein	denotes	CD8
T14961	2367-2370	Protein	denotes	CD4
T14962	2378-2381	Protein	denotes	CD8
T14963	2382-2385	Protein	denotes	CD4
T14964	2421-2424	Protein	denotes	CD4
T14965	2456-2461	Protein	denotes	Runx3
T14966	2509-2512	Protein	denotes	CD8
T14967	2513-2516	Protein	denotes	CD4
T14968	2575-2578	Protein	denotes	CD8
T14969	2579-2582	Protein	denotes	CD4
T14970	2637-2640	Protein	denotes	CD3
T14971	2647-2651	Protein	denotes	CD28
T14972	2751-2755	Protein	denotes	IL-2
T14973	2810-2815	Protein	denotes	Runx3
T14974	2819-2822	Protein	denotes	CD8
T14975	2845-2853	Negative_regulation	denotes	impaired
T14976	2871-2874	Protein	denotes	CD4
T14977	2875-2885	Gene_expression	denotes	expression
T14978	2919-2924	Protein	denotes	Runx3
T14979	3015-3028	Positive_regulation	denotes	up-regulation
T14980	3032-3036	Protein	denotes	CD25
T14981	3041-3045	Protein	denotes	CD69
T14982	3091-3102	Positive_regulation	denotes	up-regulate
T14983	3103-3107	Protein	denotes	CD25
T14984	3109-3114	Protein	denotes	Runx3
T14985	3118-3121	Protein	denotes	CD8
T14986	3144-3148	Protein	denotes	IL-2
T14987	3149-3164	Negative_regulation	denotes	supplementation
T14988	3333-3341	Negative_regulation	denotes	silenced
T14989	3342-3345	Protein	denotes	CD4
T14990	3346-3356	Gene_expression	denotes	expression
T14991	3655-3660	Protein	denotes	Runx3
T14992	3664-3667	Protein	denotes	CD8
T14993	3687-3696	Gene_expression	denotes	coexpress
T14994	3697-3700	Protein	denotes	CD4
T14995	3786-3791	Protein	denotes	Runx3
T14996	3857-3862	Protein	denotes	Runx3
T14997	3866-3869	Protein	denotes	CD8
T16116	4042-4045	Protein	denotes	CD8
T16117	4200-4209	Protein	denotes	Annexin V
T16118	4224-4227	Protein	denotes	CD8
T16119	4331-4334	Protein	denotes	CD8
T16120	4386-4395	Protein	denotes	Annexin V
T16643	5347-5350	Protein	denotes	CD8
T16644	5654-5664	Protein	denotes	Eomes-VP16
T16938	5822-5825	Protein	denotes	CD8
T16939	6846-6851	Protein	denotes	Eomes
T16940	6873-6878	Protein	denotes	Runx3
T17558	9112-9115	Protein	denotes	CD8
T17559	9205-9209	Protein	denotes	IL-2
T18115	9825-9828	Protein	denotes	CD8
T18116	9843-9848	Protein	denotes	Runx3
T18117	9903-9908	Protein	denotes	Runx3
R11595	T14953	T14954	themeOf	Runx3,deficient
R11596	T14954	T14955	causeOf	deficient,silence
R11597	T14956	T14957	themeOf	CD4,expression
R11598	T14957	T14955	themeOf	expression,silence
R11599	T14976	T14977	themeOf	CD4,expression
R11600	T14977	T14975	themeOf	expression,impaired
R11601	T14980	T14979	themeOf	CD25,up-regulation
R11602	T14981	T14979	themeOf	CD69,up-regulation
R11603	T14983	T14982	themeOf	CD25,up-regulate
R11604	T14986	T14987	themeOf	IL-2,supplementation
R11605	T14989	T14990	themeOf	CD4,expression
R11606	T14990	T14988	themeOf	expression,silenced
R11607	T14994	T14993	themeOf	CD4,coexpress

PMC:2626671 / 25277-35396 JSONTXT

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PMC:2626671 / 25277-35396 JSON TXT