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2_test

Id Subject Object Predicate Lexical cue
19139168-9405358-59674043 8341-8343 9405358 denotes 18
19139168-15051762-59674044 8345-8347 15051762 denotes 19
19139168-15516969-59674045 9400-9402 15516969 denotes 20
19139168-11786644-59674046 10743-10745 11786644 denotes 17
19139168-11786644-59674047 11155-11157 11786644 denotes 17
19139168-14673093-59674048 11159-11161 14673093 denotes 21
19139168-16273099-59674049 13377-13378 16273099 denotes 8
19139168-17195845-59674050 14147-14149 17195845 denotes 15
19139168-17195845-59674051 16471-16473 17195845 denotes 15
19139168-17242680-59674052 16475-16477 17242680 denotes 22
19139168-10544201-59674053 17540-17542 10544201 denotes 23
19139168-17222571-59674054 17621-17623 17222571 denotes 24
19139168-17646406-59674055 17699-17701 17646406 denotes 10
19139168-17981204-59674056 17795-17797 17981204 denotes 25
19139168-16273099-59674057 17987-17988 16273099 denotes 8
19139168-11786644-59674058 18114-18116 11786644 denotes 17
19139168-12464175-59674059 21026-21028 12464175 denotes 12
19139168-12464175-59674060 21155-21157 12464175 denotes 12
19139168-12796513-59674061 21297-21299 12796513 denotes 13
19139168-12464175-59674062 21923-21925 12464175 denotes 12
19139168-16273099-59674063 23271-23272 16273099 denotes 8
19139168-16273099-59674064 23351-23352 16273099 denotes 8
19139168-15084276-59674065 23354-23356 15084276 denotes 26
19139168-14605368-59674066 23437-23438 14605368 denotes 7
19139168-10716450-59674067 24191-24193 10716450 denotes 27
19139168-18635804-59674068 24283-24284 18635804 denotes 9
19139168-17195845-59674069 24801-24803 17195845 denotes 15
19139168-17195845-59674070 24939-24941 17195845 denotes 15
19139168-12464175-59674071 27536-27538 12464175 denotes 12
19139168-12796513-59674072 27540-27542 12796513 denotes 13
19139168-12464175-59674073 28174-28176 12464175 denotes 12
19139168-12796513-59674074 28178-28180 12796513 denotes 13
19139168-15516924-59674075 29956-29958 15516924 denotes 28
19139168-16273099-59674076 31052-31053 16273099 denotes 8
19139168-16510851-59674077 33729-33731 16510851 denotes 29

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T270 0-5 NN denotes Runx3
T271 6-9 CC denotes and
T272 10-15 NN denotes T-box
T273 16-24 NN denotes proteins
T274 25-34 VB denotes cooperate
T275 35-37 TO denotes to
T276 38-47 VB denotes establish
T277 48-51 DT denotes the
T278 52-67 JJ denotes transcriptional
T279 68-75 NN denotes program
T280 76-78 IN denotes of
T281 79-87 NN denotes effector
T282 88-92 NN denotes CTLs
T283 104-114 NN denotes Activation
T284 115-117 IN denotes of
T285 118-123 JJ denotes naive
T286 124-128 JJ denotes CD8+
T287 129-130 NN denotes T
T288 131-136 NN denotes cells
T289 137-141 IN denotes with
T290 142-149 NN denotes antigen
T291 150-157 VB denotes induces
T292 158-163 PRP-DOLLAR- denotes their
T293 164-179 NN denotes differentiation
T294 180-184 IN denotes into
T295 185-193 NN denotes effector
T296 194-203 JJ denotes cytolytic
T297 204-205 NN denotes T
T298 206-217 NN denotes lymphocytes
T299 218-219 -LRB- denotes (
T300 219-223 NN denotes CTLs
T301 223-224 -RRB- denotes )
T302 226-230 NN denotes CTLs
T303 231-235 VB denotes lyse
T304 236-244 JJ denotes infected
T305 245-247 CC denotes or
T306 248-256 JJ denotes aberrant
T307 257-263 NN denotes target
T308 264-269 NN denotes cells
T309 270-272 IN denotes by
T310 273-283 NN denotes exocytosis
T311 284-286 IN denotes of
T312 287-292 JJ denotes lytic
T313 293-301 NN denotes granules
T314 302-312 VB denotes containing
T315 313-316 DT denotes the
T316 317-329 JJ denotes pore-forming
T317 330-337 NN denotes protein
T318 338-346 NN denotes perforin
T319 347-350 CC denotes and
T320 351-352 DT denotes a
T321 353-359 NN denotes family
T322 360-362 IN denotes of
T323 363-372 NN denotes proteases
T324 373-379 VB denotes termed
T325 380-389 NN denotes granzymes
T326 391-393 PRP denotes We
T327 394-398 VB denotes show
T328 399-403 IN denotes that
T329 404-412 NN denotes effector
T330 413-416 NN denotes CTL
T331 417-432 NN denotes differentiation
T332 433-439 VB denotes occurs
T333 440-442 IN denotes in
T334 443-446 CD denotes two
T335 447-457 JJ denotes sequential
T336 458-464 NN denotes phases
T337 465-467 FW denotes in
T338 468-473 FW denotes vitro
T339 473-474 -COMMA- denotes ,
T340 475-488 VB denotes characterized
T341 489-491 IN denotes by
T342 492-497 JJ denotes early
T343 498-507 NN denotes induction
T344 508-510 IN denotes of
T345 511-516 NN denotes T-bet
T346 517-520 CC denotes and
T347 521-525 JJ denotes late
T348 526-535 NN denotes induction
T349 536-538 IN denotes of
T350 539-551 NN denotes Eomesodermin
T351 552-553 -LRB- denotes (
T352 553-558 NN denotes Eomes
T353 558-559 -RRB- denotes )
T354 559-560 -COMMA- denotes ,
T355 561-566 NN denotes T-box
T356 567-580 NN denotes transcription
T357 581-588 NN denotes factors
T358 589-593 WDT denotes that
T359 594-602 VB denotes regulate
T360 603-606 DT denotes the
T361 607-612 JJ denotes early
T362 613-616 CC denotes and
T363 617-621 JJ denotes late
T364 622-628 NN denotes phases
T365 629-631 IN denotes of
T366 632-642 NN denotes interferon
T367 643-644 -LRB- denotes (
T368 644-647 NN denotes IFN
T369 647-648 -RRB- denotes )
T370 649-650 NN denotes γ
T371 651-661 NN denotes expression
T372 661-662 -COMMA- denotes ,
T373 663-675 RB denotes respectively
T374 677-679 IN denotes In
T375 680-688 NN denotes addition
T376 688-689 -COMMA- denotes ,
T377 690-692 PRP denotes we
T378 693-704 VB denotes demonstrate
T379 705-706 DT denotes a
T380 707-715 JJ denotes critical
T381 716-720 NN denotes role
T382 721-724 IN denotes for
T383 725-728 DT denotes the
T384 729-742 NN denotes transcription
T385 743-749 NN denotes factor
T386 750-755 NN denotes Runx3
T387 756-758 IN denotes in
T388 759-762 NN denotes CTL
T389 763-778 NN denotes differentiation
T390 780-785 NN denotes Runx3
T391 786-795 VB denotes regulates
T392 796-801 NN denotes Eomes
T393 802-812 NN denotes expression
T394 813-815 RB denotes as
T395 816-820 RB denotes well
T396 821-823 IN denotes as
T397 824-834 NN denotes expression
T398 835-837 IN denotes of
T399 838-843 CD denotes three
T400 844-852 JJ denotes cardinal
T401 853-860 NN denotes markers
T402 861-863 IN denotes of
T403 864-867 DT denotes the
T404 868-876 NN denotes effector
T405 877-880 NN denotes CTL
T406 881-888 NN denotes program
T407 888-889 -COLON- denotes :
T408 890-895 NN denotes IFN-γ
T409 895-896 -COMMA- denotes ,
T410 897-905 NN denotes perforin
T411 905-906 -COMMA- denotes ,
T412 907-910 CC denotes and
T413 911-919 NN denotes granzyme
T414 920-922 NNP denotes B.
T415 923-926 PRP-DOLLAR- denotes Our
T416 927-931 NN denotes data
T417 932-937 VB denotes point
T418 938-940 TO denotes to
T419 941-944 DT denotes the
T420 945-954 NN denotes existence
T421 955-957 IN denotes of
T422 958-960 DT denotes an
T423 961-970 JJ denotes elaborate
T424 971-986 JJ denotes transcriptional
T425 987-994 NN denotes network
T426 995-997 IN denotes in
T427 998-1003 WDT denotes which
T428 1004-1009 NN denotes Runx3
T429 1010-1019 RB denotes initially
T430 1020-1027 VB denotes induces
T431 1028-1031 CC denotes and
T432 1032-1036 RB denotes then
T433 1037-1047 VB denotes cooperates
T434 1048-1052 IN denotes with
T435 1053-1058 NN denotes T-box
T436 1059-1072 NN denotes transcription
T437 1073-1080 NN denotes factors
T438 1081-1083 TO denotes to
T439 1084-1092 VB denotes regulate
T440 1093-1097 NN denotes gene
T441 1098-1111 NN denotes transcription
T442 1112-1114 IN denotes in
T443 1115-1130 VB denotes differentiating
T444 1131-1135 NN denotes CTLs
T1503 1138-1143 JJ denotes Naive
T1504 1144-1150 NN denotes CD8+ T
T1505 1151-1156 NN denotes cells
T1506 1157-1170 VB denotes differentiate
T1507 1171-1175 IN denotes into
T1508 1176-1184 NN denotes effector
T1509 1185-1189 NN denotes CTLs
T1510 1190-1194 IN denotes with
T1511 1195-1198 DT denotes the
T1512 1199-1206 NN denotes ability
T1513 1207-1209 TO denotes to
T1514 1210-1214 VB denotes lyse
T1515 1215-1230 JJ denotes antigen-bearing
T1516 1231-1237 NN denotes target
T1517 1238-1243 NN denotes cells
T1518 1244-1246 IN denotes by
T1519 1247-1257 NN denotes exocytosis
T1520 1258-1260 IN denotes of
T1521 1261-1266 JJ denotes lytic
T1522 1267-1275 NN denotes granules
T1523 1276-1286 VB denotes containing
T1524 1287-1295 NN denotes perforin
T1525 1296-1299 CC denotes and
T1526 1300-1309 NN denotes granzymes
T1527 1309-1310 -COMMA- denotes ,
T1528 1311-1314 CC denotes and
T1529 1315-1317 TO denotes to
T1530 1318-1325 VB denotes produce
T1531 1326-1338 JJ denotes inflammatory
T1532 1339-1348 NN denotes cytokines
T1533 1349-1353 JJ denotes such
T1534 1354-1356 IN denotes as
T1535 1357-1362 NN denotes IFN-γ
T1536 1363-1366 CC denotes and
T1537 1367-1370 NN denotes TNF
T1538 1371-1375 IN denotes upon
T1539 1376-1389 NN denotes restimulation
T1540 1390-1397 IN denotes through
T1541 1398-1401 DT denotes the
T1542 1402-1405 NN denotes TCR
T1543 1406-1407 -LRB- denotes (
T1544 1407-1411 CD denotes 1, 2
T1545 1411-1412 -RRB- denotes )
T1546 1414-1416 FW denotes In
T1547 1417-1421 FW denotes vivo
T1548 1422-1433 NN denotes experiments
T1549 1434-1438 VB denotes have
T1550 1439-1449 VB denotes elucidated
T1551 1450-1454 JJ denotes many
T1552 1455-1463 JJ denotes critical
T1553 1464-1474 NN denotes parameters
T1554 1475-1484 VB denotes governing
T1555 1485-1488 DT denotes the
T1556 1489-1500 NN denotes development
T1557 1501-1504 CC denotes and
T1558 1505-1514 NN denotes evolution
T1559 1515-1517 IN denotes of
T1560 1518-1525 JJ denotes primary
T1561 1526-1529 NN denotes CTL
T1562 1530-1539 NN denotes responses
T1563 1540-1541 -LRB- denotes (
T1564 1541-1545 CD denotes 3, 4
T1565 1545-1546 -RRB- denotes )
T1566 1548-1550 IN denotes In
T1567 1551-1555 DT denotes this
T1568 1556-1561 NN denotes study
T1569 1561-1562 -COMMA- denotes ,
T1570 1563-1565 PRP denotes we
T1571 1566-1570 VB denotes have
T1572 1571-1575 VB denotes used
T1573 1576-1578 FW denotes in
T1574 1579-1584 FW denotes vitro
T1575 1585-1592 NN denotes systems
T1576 1593-1597 JJ denotes such
T1577 1598-1600 IN denotes as
T1578 1601-1606 DT denotes those
T1579 1607-1616 VB denotes developed
T1580 1617-1619 TO denotes to
T1581 1620-1625 VB denotes study
T1582 1626-1632 NN denotes CD4+ T
T1583 1633-1637 NN denotes cell
T1584 1638-1653 NN denotes differentiation
T1585 1654-1656 TO denotes to
T1586 1657-1663 VB denotes define
T1587 1664-1667 DT denotes the
T1588 1668-1677 JJ denotes molecular
T1589 1678-1683 NN denotes basis
T1590 1684-1686 IN denotes of
T1591 1687-1695 NN denotes effector
T1592 1696-1699 NN denotes CTL
T1593 1700-1715 NN denotes differentiation
T1594 1716-1717 -LRB- denotes (
T1595 1717-1721 NN denotes 5, 6
T1596 1721-1722 -RRB- denotes )
T1597 1722-1727 NN denotes . The
T1598 1728-1733 NN denotes T-box
T1599 1734-1747 NN denotes transcription
T1600 1748-1755 NN denotes factors
T1601 1756-1768 NN denotes Eomesodermin
T1602 1769-1770 -LRB- denotes (
T1603 1770-1775 NN denotes Eomes
T1604 1775-1776 -RRB- denotes )
T1605 1777-1780 CC denotes and
T1606 1781-1786 NN denotes T-bet
T1607 1787-1790 VB denotes are
T1608 1791-1797 VB denotes needed
T1609 1798-1801 IN denotes for
T1610 1802-1811 JJ denotes important
T1611 1812-1819 NN denotes aspects
T1612 1820-1822 IN denotes of
T1613 1823-1831 NN denotes effector
T1614 1832-1835 CC denotes and
T1615 1836-1842 NN denotes memory
T1616 1843-1846 NN denotes CTL
T1617 1847-1862 NN denotes differentiation
T1618 1863-1864 -LRB- denotes (
T1619 1864-1865 CD denotes 7
T1620 1865-1866 -RRB- denotes )
T1621 1868-1870 IN denotes In
T1622 1871-1881 JJ denotes uninfected
T1623 1882-1886 NN denotes mice
T1624 1886-1887 -COMMA- denotes ,
T1625 1888-1896 NN denotes compound
T1626 1897-1905 NN denotes deletion
T1627 1906-1908 IN denotes of
T1628 1909-1918 NN denotes the Tbx21
T1629 1919-1920 -LRB- denotes (
T1630 1920-1928 VB denotes encoding
T1631 1929-1934 NN denotes T-bet
T1632 1934-1935 -RRB- denotes )
T1633 1936-1958 NN denotes and eomesodermin genes
T1634 1959-1961 VB denotes is
T1635 1962-1972 VB denotes associated
T1636 1973-1977 IN denotes with
T1637 1978-1979 DT denotes a
T1638 1980-1989 JJ denotes selective
T1639 1990-1994 NN denotes loss
T1640 1995-1997 IN denotes of
T1641 1998-2004 NN denotes CD8+ T
T1642 2005-2010 NN denotes cells
T1643 2011-2015 IN denotes with
T1644 2016-2018 DT denotes an
T1645 2019-2030 NN denotes IL-2Rβ–high
T1646 2030-2031 -COMMA- denotes ,
T1647 2032-2038 NN denotes memory
T1648 2039-2048 NN denotes phenotype
T1649 2049-2050 -LRB- denotes (
T1650 2050-2051 CD denotes 8
T1651 2051-2052 -RRB- denotes )
T1652 2054-2058 NN denotes Mice
T1653 2059-2068 JJ denotes deficient
T1654 2069-2072 IN denotes for
T1655 2073-2077 CC denotes both
T1656 2078-2083 NN denotes T-bet
T1657 2084-2087 CC denotes and
T1658 2088-2093 NN denotes Eomes
T1659 2094-2096 IN denotes in
T1660 2097-2098 NN denotes T
T1661 2099-2104 NN denotes cells
T1662 2105-2109 VB denotes have
T1663 2110-2118 VB denotes impaired
T1664 2119-2129 NN denotes expression
T1665 2130-2132 IN denotes of
T1666 2133-2142 JJ denotes cytolytic
T1667 2143-2152 NN denotes mediators
T1668 2152-2153 -COMMA- denotes ,
T1669 2154-2162 VB denotes manifest
T1670 2163-2167 JJ denotes poor
T1671 2168-2177 JJ denotes cytolytic
T1672 2178-2186 NN denotes activity
T1673 2186-2187 -COMMA- denotes ,
T1674 2188-2191 CC denotes and
T1675 2192-2196 VB denotes fail
T1676 2197-2199 TO denotes to
T1677 2200-2207 VB denotes control
T1678 2208-2213 JJ denotes acute
T1679 2214-2225 JJ denotes lymphocytic
T1680 2226-2242 NN denotes choriomeningitis
T1681 2243-2248 NN denotes virus
T1682 2249-2258 NN denotes infection
T1683 2259-2260 -LRB- denotes (
T1684 2260-2261 CD denotes 9
T1685 2261-2262 -RRB- denotes )
T1686 2264-2276 RB denotes Nevertheless
T1687 2276-2277 -COMMA- denotes ,
T1688 2278-2281 DT denotes the
T1689 2282-2290 JJ denotes specific
T1690 2291-2296 NN denotes roles
T1691 2297-2299 IN denotes of
T1692 2300-2305 NN denotes T-bet
T1693 2306-2309 CC denotes and
T1694 2310-2315 NN denotes Eomes
T1695 2316-2318 IN denotes in
T1696 2319-2325 JJ denotes clonal
T1697 2326-2335 NN denotes expansion
T1698 2336-2339 CC denotes and
T1699 2340-2343 NN denotes CTL
T1700 2344-2359 NN denotes differentiation
T1701 2360-2364 VB denotes have
T1702 2365-2368 RB denotes not
T1703 2369-2372 RB denotes yet
T1704 2373-2377 VB denotes been
T1705 2378-2386 VB denotes resolved
T1706 2386-2387 -COLON- denotes :
T1707 2388-2390 IN denotes in
T1708 2391-2401 JJ denotes particular
T1709 2401-2402 -COMMA- denotes ,
T1710 2403-2405 PRP denotes it
T1711 2406-2408 VB denotes is
T1712 2409-2412 RB denotes not
T1713 2413-2418 VB denotes known
T1714 2419-2426 IN denotes whether
T1715 2427-2432 DT denotes these
T1716 2433-2446 NN denotes transcription
T1717 2447-2454 NN denotes factors
T1718 2455-2463 VB denotes function
T1719 2464-2475 RB denotes redundantly
T1720 2476-2478 TO denotes to
T1721 2479-2486 VB denotes control
T1722 2487-2495 NN denotes effector
T1723 2496-2502 NN denotes CD8+ T
T1724 2503-2507 NN denotes cell
T1725 2508-2523 NN denotes differentiation
T1726 2523-2524 -COMMA- denotes ,
T1727 2525-2528 CC denotes and
T1728 2529-2536 IN denotes whether
T1729 2537-2541 PRP denotes they
T1730 2542-2544 VB denotes do
T1731 2545-2547 RB denotes so
T1732 2548-2556 RB denotes directly
T1733 2557-2559 IN denotes by
T1734 2560-2569 VB denotes targeting
T1735 2570-2578 JJ denotes specific
T1736 2579-2587 NN denotes effector
T1737 2588-2596 NN denotes cytokine
T1738 2597-2600 CC denotes and
T1739 2601-2610 JJ denotes cytolytic
T1740 2611-2622 NN denotes genes. Runx
T1741 2623-2631 NN denotes proteins
T1742 2631-2632 -COMMA- denotes ,
T1743 2633-2634 DT denotes a
T1744 2635-2641 NN denotes family
T1745 2642-2644 IN denotes of
T1746 2645-2650 CD denotes three
T1747 2651-2662 JJ denotes DNA-binding
T1748 2663-2676 NN denotes transcription
T1749 2677-2684 NN denotes factors
T1750 2684-2685 -COMMA- denotes ,
T1751 2686-2693 NN denotes control
T1752 2694-2703 NN denotes thymocyte
T1753 2704-2719 NN denotes differentiation
T1754 2720-2723 CC denotes and
T1755 2724-2727 DT denotes the
T1756 2728-2735 NN denotes CD4/CD8
T1757 2736-2743 NN denotes lineage
T1758 2744-2752 NN denotes decision
T1759 2753-2754 -LRB- denotes (
T1760 2754-2759 CD denotes 10–13
T1761 2759-2760 -RRB- denotes )
T1762 2762-2767 NN denotes Runx3
T1763 2768-2771 CC denotes and
T1764 2772-2780 NN denotes perforin
T1765 2781-2785 NN denotes mRNA
T1766 2786-2789 VB denotes are
T1767 2790-2799 VB denotes expressed
T1768 2800-2802 IN denotes by
T1769 2803-2818 JJ denotes double-positive
T1770 2819-2820 -LRB- denotes (
T1771 2820-2822 NN denotes DP
T1772 2822-2823 -RRB- denotes )
T1773 2824-2834 NN denotes thymocytes
T1774 2835-2838 CC denotes and
T1775 2839-2859 JJ denotes CD8+ single-positive
T1776 2860-2861 -LRB- denotes (
T1777 2861-2863 NN denotes SP
T1778 2863-2864 -RRB- denotes )
T1779 2865-2875 NN denotes thymocytes
T1780 2876-2879 CC denotes but
T1781 2880-2883 RB denotes not
T1782 2884-2886 IN denotes in
T1783 2887-2894 NN denotes CD4+ SP
T1784 2895-2900 NN denotes cells
T1785 2901-2902 -LRB- denotes (
T1786 2902-2904 CD denotes 14
T1787 2904-2905 -RRB- denotes )
T1788 2907-2915 IN denotes Although
T1789 2916-2921 NN denotes Runx3
T1790 2922-2924 VB denotes is
T1791 2925-2928 RB denotes not
T1792 2929-2938 VB denotes expressed
T1793 2939-2941 IN denotes in
T1794 2942-2947 JJ denotes naive
T1795 2948-2954 NN denotes CD4+ T
T1796 2955-2960 NN denotes cells
T1797 2960-2961 -COMMA- denotes ,
T1798 2962-2965 PRP-DOLLAR- denotes its
T1799 2966-2976 NN denotes expression
T1800 2977-2979 VB denotes is
T1801 2980-2992 VB denotes up-regulated
T1802 2993-2999 IN denotes during
T1803 3000-3003 NN denotes Th1
T1804 3004-3008 NN denotes cell
T1805 3009-3024 NN denotes differentiation
T1806 3024-3025 -COMMA- denotes ,
T1807 3026-3029 CC denotes and
T1808 3030-3035 NN denotes Runx3
T1809 3036-3046 VB denotes influences
T1810 3047-3050 NN denotes Th1
T1811 3051-3055 NN denotes cell
T1812 3056-3071 NN denotes differentiation
T1813 3072-3075 CC denotes and
T1814 3076-3084 NN denotes function
T1815 3085-3092 IN denotes through
T1816 3093-3099 JJ denotes direct
T1817 3100-3110 NN denotes regulation
T1818 3111-3113 IN denotes of
T1819 3114-3139 NN denotes the Il4 and Ifng cytokine
T1820 3140-3145 NN denotes genes
T1821 3146-3147 -LRB- denotes (
T1822 3147-3153 CD denotes 15, 16
T1823 3153-3154 -RRB- denotes )
T1824 3156-3158 IN denotes In
T1825 3159-3167 NN denotes contrast
T1826 3167-3168 -COMMA- denotes ,
T1827 3169-3172 DT denotes all
T1828 3173-3178 CD denotes three
T1829 3179-3183 NN denotes Runx
T1830 3184-3192 NN denotes proteins
T1831 3193-3196 VB denotes are
T1832 3197-3206 VB denotes expressed
T1833 3207-3209 IN denotes in
T1834 3210-3216 JJ denotes mature
T1835 3217-3223 NN denotes CD8+ T
T1836 3224-3229 NN denotes cells
T1837 3230-3231 -LRB- denotes (
T1838 3231-3237 CD denotes 10, 12
T1839 3237-3238 -RRB- denotes )
T1840 3238-3239 -COMMA- denotes ,
T1841 3240-3243 CC denotes and
T1842 3244-3259 JJ denotes Runx3-deficient
T1843 3260-3266 NN denotes CD8+ T
T1844 3267-3272 NN denotes cells
T1845 3273-3277 VB denotes show
T1846 3278-3285 VB denotes reduced
T1847 3286-3295 JJ denotes cytolytic
T1848 3296-3304 NN denotes activity
T1849 3305-3306 -LRB- denotes (
T1850 3306-3312 CD denotes 12, 13
T1851 3312-3313 -RRB- denotes )
T1852 3315-3317 PRP denotes We
T1853 3318-3327 RB denotes therefore
T1854 3328-3334 VB denotes tested
T1855 3335-3342 IN denotes whether
T1856 3343-3348 NN denotes Runx3
T1857 3349-3359 VB denotes influenced
T1858 3360-3369 JJ denotes cytolytic
T1859 3370-3371 NN denotes T
T1860 3372-3376 NN denotes cell
T1861 3377-3396 NN denotes differentiation. In
T1862 3397-3401 DT denotes this
T1863 3402-3408 NN denotes report
T1864 3408-3409 -COMMA- denotes ,
T1865 3410-3412 PRP denotes we
T1866 3413-3417 VB denotes show
T1867 3418-3422 IN denotes that
T1868 3423-3428 NN denotes Runx3
T1869 3429-3432 CC denotes and
T1870 3433-3438 NN denotes T-box
T1871 3439-3446 NN denotes factors
T1872 3447-3462 RB denotes synergistically
T1873 3463-3471 VB denotes regulate
T1874 3472-3475 NN denotes CTL
T1875 3476-3491 NN denotes differentiation
T1876 3492-3495 CC denotes and
T1877 3496-3504 NN denotes function
T1878 3506-3511 NN denotes T-bet
T1879 3512-3514 VB denotes is
T1880 3515-3522 VB denotes induced
T1881 3523-3530 RB denotes quickly
T1882 3531-3535 IN denotes upon
T1883 3536-3539 NN denotes TCR
T1884 3540-3551 NN denotes stimulation
T1885 3552-3555 CC denotes and
T1886 3556-3558 VB denotes is
T1887 3559-3567 VB denotes required
T1888 3568-3571 IN denotes for
T1889 3572-3577 JJ denotes early
T1890 3578-3589 NN denotes programming
T1891 3590-3592 IN denotes of
T1892 3593-3601 NN denotes cytokine
T1893 3602-3612 NN denotes production
T1894 3613-3614 -LRB- denotes (
T1895 3614-3616 CD denotes 17
T1896 3616-3617 -RRB- denotes )
T1897 3617-3618 -COMMA- denotes ,
T1898 3619-3626 IN denotes whereas
T1899 3627-3632 NN denotes Eomes
T1900 3633-3635 VB denotes is
T1901 3636-3643 VB denotes induced
T1902 3644-3649 RB denotes later
T1903 3650-3656 IN denotes during
T1904 3657-3672 NN denotes differentiation
T1905 3673-3676 CC denotes and
T1906 3677-3685 VB denotes sustains
T1907 3686-3691 NN denotes IFN-γ
T1908 3692-3702 NN denotes expression
T1909 3704-3709 NN denotes Runx3
T1910 3710-3712 VB denotes is
T1911 3713-3721 VB denotes required
T1912 3722-3725 IN denotes for
T1913 3726-3731 NN denotes Eomes
T1914 3732-3735 CC denotes and
T1915 3736-3744 NN denotes perforin
T1916 3745-3755 NN denotes expression
T1917 3755-3756 -COMMA- denotes ,
T1918 3757-3760 CC denotes and
T1919 3761-3765 DT denotes both
T1920 3766-3771 NN denotes Eomes
T1921 3772-3775 CC denotes and
T1922 3776-3781 NN denotes Runx3
T1923 3782-3786 VB denotes bind
T1924 3787-3789 IN denotes at
T1925 3790-3804 NN denotes the Prf1 locus
T1926 3804-3805 -COLON- denotes ;
T1927 3806-3808 IN denotes in
T1928 3809-3817 NN denotes contrast
T1929 3817-3818 -COMMA- denotes ,
T1930 3819-3827 NN denotes perforin
T1931 3828-3838 NN denotes expression
T1932 3839-3841 VB denotes is
T1933 3842-3852 JJ denotes unaffected
T1934 3853-3855 IN denotes in
T1935 3856-3871 JJ denotes T-bet–deficient
T1936 3872-3877 NN denotes cells
T1937 3879-3880 NN denotes T
T1938 3881-3886 NN denotes cells
T1939 3887-3894 VB denotes lacking
T1940 3895-3900 NN denotes Runx3
T1941 3901-3905 NN denotes show
T1942 3906-3915 VB denotes decreased
T1943 3916-3926 NN denotes expression
T1944 3927-3929 IN denotes of
T1945 3930-3935 NN denotes IFN-γ
T1946 3936-3939 CC denotes and
T1947 3940-3948 NN denotes granzyme
T1948 3949-3950 NN denotes B
T1949 3950-3951 -COMMA- denotes ,
T1950 3952-3955 CC denotes and
T1951 3956-3961 NN denotes Runx3
T1952 3962-3966 RB denotes also
T1953 3967-3972 VB denotes binds
T1954 3973-3976 DT denotes the
T1955 3977-3985 NN denotes promoter
T1956 3986-3993 NN denotes regions
T1957 3994-3996 IN denotes of
T1958 3997-4020 NN denotes the Ifng and Gzmb genes
T1959 4022-4034 RB denotes Collectively
T1960 4034-4035 -COMMA- denotes ,
T1961 4036-4041 DT denotes these
T1962 4042-4049 NN denotes results
T1963 4050-4057 VB denotes provide
T1964 4058-4066 NN denotes evidence
T1965 4067-4070 IN denotes for
T1966 4071-4072 DT denotes a
T1967 4073-4080 JJ denotes complex
T1968 4081-4096 JJ denotes transcriptional
T1969 4097-4104 NN denotes network
T1970 4105-4107 IN denotes in
T1971 4108-4113 WDT denotes which
T1972 4114-4119 NN denotes Runx3
T1973 4120-4122 VB denotes is
T1974 4123-4124 DT denotes a
T1975 4125-4132 JJ denotes primary
T1976 4133-4142 NN denotes regulator
T1977 4143-4161 NN denotes of Gzmb expression
T1978 4162-4165 CC denotes but
T1979 4166-4176 VB denotes synergizes
T1980 4177-4181 IN denotes with
T1981 4182-4187 NN denotes T-bet
T1982 4188-4191 CC denotes and
T1983 4192-4197 NN denotes Eomes
T1984 4197-4198 -COMMA- denotes ,
T1985 4199-4211 RB denotes respectively
T1986 4211-4212 -COMMA- denotes ,
T1987 4213-4215 TO denotes to
T1988 4216-4223 VB denotes promote
T1989 4224-4237 NN denotes transcription
T1990 4238-4240 IN denotes of
T1991 4241-4264 NN denotes the Ifng and Prf1 genes
T2913 4291-4292 DT denotes A
T2914 4293-4297 NN denotes cell
T2915 4298-4305 NN denotes culture
T2916 4306-4312 NN denotes system
T2917 4313-4315 TO denotes to
T2918 4316-4323 VB denotes monitor
T2919 4324-4332 NN denotes effector
T2920 4333-4336 NN denotes CTL
T2921 4337-4352 NN denotes differentiation
T2922 4353-4355 PRP denotes We
T2923 4356-4360 VB denotes used
T2924 4361-4362 DT denotes a
T2925 4363-4369 JJ denotes simple
T2926 4370-4374 NN denotes cell
T2927 4375-4382 NN denotes culture
T2928 4383-4389 NN denotes system
T2929 4390-4392 TO denotes to
T2930 4393-4400 VB denotes examine
T2931 4401-4404 DT denotes the
T2932 4405-4413 NN denotes kinetics
T2933 4414-4416 IN denotes of
T2934 4417-4425 NN denotes effector
T2935 4426-4430 NN denotes gene
T2936 4431-4441 NN denotes expression
T2937 4442-4448 IN denotes during
T2938 4449-4453 JJ denotes CD8+
T2939 4454-4455 NN denotes T
T2940 4456-4460 NN denotes cell
T2941 4461-4476 NN denotes differentiation
T2942 4478-4483 JJ denotes Naive
T2943 4484-4488 JJ denotes CD8+
T2944 4489-4490 NN denotes T
T2945 4491-4496 NN denotes cells
T2946 4497-4501 IN denotes from
T2947 4502-4505 NN denotes P14
T2948 4506-4509 NN denotes TCR
T2949 4510-4520 JJ denotes transgenic
T2950 4521-4525 NN denotes mice
T2951 4526-4530 VB denotes were
T2952 4531-4540 VB denotes activated
T2953 4541-4544 IN denotes for
T2954 4545-4546 CD denotes 2
T2955 4547-4548 NN denotes d
T2956 4549-4553 IN denotes with
T2957 4554-4562 NN denotes anti-CD3
T2958 4563-4566 CC denotes and
T2959 4567-4576 NN denotes anti-CD28
T2960 4577-4579 CC denotes or
T2961 4580-4584 IN denotes with
T2962 4585-4592 JJ denotes splenic
T2963 4593-4597 NN denotes APCs
T2964 4598-4600 IN denotes in
T2965 4601-4604 DT denotes the
T2966 4605-4613 NN denotes presence
T2967 4614-4616 IN denotes of
T2968 4617-4621 NN denotes Gp33
T2969 4622-4629 NN denotes peptide
T2970 4629-4630 -COMMA- denotes ,
T2971 4631-4634 CC denotes and
T2972 4635-4639 VB denotes were
T2973 4640-4648 VB denotes cultured
T2974 4649-4651 IN denotes in
T2975 4652-4657 NN denotes media
T2976 4658-4668 VB denotes containing
T2977 4669-4672 CD denotes 100
T2978 4673-4677 NN denotes U/ml
T2979 4678-4680 IN denotes of
T2980 4681-4692 JJ denotes recombinant
T2981 4693-4698 JJ denotes human
T2982 4699-4703 NN denotes IL-2
T2983 4704-4705 -LRB- denotes (
T2984 4705-4711 NN denotes rhIL-2
T2985 4711-4712 -RRB- denotes )
T2986 4714-4716 PRP denotes We
T2987 4717-4721 VB denotes used
T2988 4722-4725 NN denotes TCR
T2989 4726-4736 JJ denotes transgenic
T2990 4737-4741 NN denotes mice
T2991 4742-4745 IN denotes for
T2992 4746-4751 DT denotes these
T2993 4752-4763 NN denotes experiments
T2994 4764-4771 IN denotes because
T2995 4772-4776 PRP denotes they
T2996 4777-4784 VB denotes provide
T2997 4785-4786 DT denotes a
T2998 4787-4795 JJ denotes reliable
T2999 4796-4802 NN denotes source
T3000 4803-4805 IN denotes of
T3001 4806-4810 JJ denotes CD8+
T3002 4811-4812 NN denotes T
T3003 4813-4818 NN denotes cells
T3004 4819-4823 WDT denotes that
T3005 4824-4827 VB denotes are
T3006 4828-4833 RB denotes truly
T3007 4834-4839 JJ denotes naive
T3008 4839-4840 -COLON- denotes ;
T3009 4841-4848 RB denotes however
T3010 4848-4849 -COMMA- denotes ,
T3011 4850-4852 PRP denotes we
T3012 4853-4858 VB denotes chose
T3013 4859-4862 RB denotes not
T3014 4863-4865 TO denotes to
T3015 4866-4875 VB denotes stimulate
T3016 4876-4881 NN denotes cells
T3017 4882-4886 IN denotes with
T3018 4887-4894 NN denotes antigen
T3019 4895-4897 IN denotes in
T3020 4898-4902 JJ denotes most
T3021 4903-4914 NN denotes experiments
T3022 4915-4917 RB denotes so
T3023 4918-4920 IN denotes as
T3024 4921-4923 TO denotes to
T3025 4924-4929 VB denotes avoid
T3026 4930-4943 NN denotes contamination
T3027 4944-4948 IN denotes with
T3028 4949-4957 NN denotes proteins
T3029 4958-4961 CC denotes and
T3030 4962-4969 JJ denotes nucleic
T3031 4970-4975 NN denotes acids
T3032 4976-4983 VB denotes derived
T3033 4984-4988 IN denotes from
T3034 4989-4993 NN denotes APCs
T3035 4995-5000 EX denotes There
T3036 5001-5005 VB denotes were
T3037 5006-5010 RB denotes only
T3038 5011-5016 JJ denotes minor
T3039 5017-5028 NN denotes differences
T3040 5029-5031 IN denotes in
T3041 5032-5036 NN denotes gene
T3042 5037-5047 NN denotes expression
T3043 5048-5054 IN denotes during
T3044 5055-5070 NN denotes differentiation
T3045 5071-5078 VB denotes induced
T3046 5079-5081 IN denotes by
T3047 5082-5093 NN denotes antigen/APC
T3048 5094-5100 IN denotes versus
T3049 5101-5119 JJ denotes anti-CD3/anti-CD28
T3050 5119-5120 -COMMA- denotes ,
T3051 5121-5124 CC denotes and
T3052 5125-5128 DT denotes the
T3053 5129-5134 JJ denotes major
T3054 5135-5146 NN denotes conclusions
T3055 5147-5156 VB denotes presented
T3056 5157-5159 IN denotes in
T3057 5160-5164 DT denotes this
T3058 5165-5171 NN denotes report
T3059 5172-5175 VB denotes are
T3060 5176-5179 DT denotes the
T3061 5180-5184 JJ denotes same
T3062 5185-5188 IN denotes for
T3063 5189-5193 DT denotes both
T3064 5194-5204 VB denotes activating
T3065 5205-5215 NN denotes conditions
T3066 5217-5222 IN denotes Under
T3067 5223-5226 PRP-DOLLAR- denotes our
T3068 5227-5234 NN denotes culture
T3069 5235-5245 NN denotes conditions
T3070 5245-5246 -COMMA- denotes ,
T3071 5247-5256 VB denotes activated
T3072 5257-5261 JJ denotes CD8+
T3073 5262-5263 NN denotes T
T3074 5264-5269 NN denotes cells
T3075 5270-5278 VB denotes expanded
T3076 5279-5292 RB denotes exponentially
T3077 5293-5296 CC denotes and
T3078 5297-5308 VB denotes accumulated
T3079 5309-5312 IN denotes for
T3080 5313-5314 JJ denotes >
T3081 5314-5315 CD denotes 8
T3082 5316-5317 NN denotes d
T3083 5319-5321 PRP denotes We
T3084 5322-5329 VB denotes limited
T3085 5330-5333 PRP-DOLLAR- denotes our
T3086 5334-5342 NN denotes analysis
T3087 5343-5345 TO denotes to
T3088 5346-5349 DT denotes the
T3089 5350-5355 JJ denotes first
T3090 5356-5359 CD denotes 6–8
T3091 5360-5361 NN denotes d
T3092 5362-5367 IN denotes after
T3093 5368-5378 NN denotes activation
T3094 5378-5379 -COMMA- denotes ,
T3095 5380-5381 DT denotes a
T3096 5382-5388 NN denotes period
T3097 5389-5393 WDT denotes that
T3098 5394-5403 VB denotes coincides
T3099 5404-5408 IN denotes with
T3100 5409-5415 JJ denotes clonal
T3101 5416-5425 NN denotes expansion
T3102 5426-5428 IN denotes of
T3103 5429-5433 JJ denotes CD8+
T3104 5434-5435 NN denotes T
T3105 5436-5441 NN denotes cells
T3106 5442-5447 IN denotes after
T3107 5448-5458 NN denotes activation
T3108 5459-5461 FW denotes in
T3109 5462-5466 FW denotes vivo
T3825 5469-5477 JJ denotes Distinct
T3826 5478-5488 NN denotes expression
T3827 5489-5497 NN denotes kinetics
T3828 5498-5500 IN denotes of
T3829 5501-5509 NN denotes perforin
T3830 5510-5513 CC denotes and
T3831 5514-5522 NN denotes granzyme
T3832 5523-5524 NN denotes B
T3833 5525-5531 IN denotes during
T3834 5532-5535 NN denotes CTL
T3835 5536-5547 NN denotes development
T3836 5548-5550 IN denotes in
T3837 5551-5558 NN denotes culture
T3838 5559-5562 PRP-DOLLAR- denotes Our
T3839 5563-5574 NN denotes experiments
T3840 5575-5583 VB denotes revealed
T3841 5584-5589 JJ denotes clear
T3842 5590-5601 NN denotes differences
T3843 5602-5604 IN denotes in
T3844 5605-5608 DT denotes the
T3845 5609-5617 NN denotes kinetics
T3846 5618-5620 IN denotes of
T3847 5621-5629 NN denotes perforin
T3848 5629-5630 -COMMA- denotes ,
T3849 5631-5639 NN denotes granzyme
T3850 5640-5641 NN denotes B
T3851 5641-5642 -COMMA- denotes ,
T3852 5643-5646 CC denotes and
T3853 5647-5655 NN denotes cytokine
T3854 5656-5666 NN denotes expression
T3855 5667-5673 IN denotes during
T3856 5674-5678 JJ denotes CD8+
T3857 5679-5680 NN denotes T
T3858 5681-5685 NN denotes cell
T3859 5686-5696 NN denotes activation
T3860 5697-5698 -LRB- denotes (
T3861 5698-5702 NN denotes Fig.
T3862 5703-5704 CD denotes 1
T3863 5704-5705 -RRB- denotes )
T3864 5707-5712 JJ denotes Naive
T3865 5713-5714 NN denotes T
T3866 5715-5720 NN denotes cells
T3867 5721-5727 VB denotes showed
T3868 5728-5738 JJ denotes detectable
T3869 5739-5749 NN denotes expression
T3870 5750-5752 IN denotes of
T3871 5753-5761 NN denotes perforin
T3872 5762-5766 NN denotes mRNA
T3873 5767-5769 RB denotes as
T3874 5770-5774 RB denotes well
T3875 5775-5777 IN denotes as
T3876 5778-5786 NN denotes perforin
T3877 5787-5794 NN denotes protein
T3878 5795-5796 -LRB- denotes (
T3879 5796-5800 NN denotes Fig.
T3880 5801-5802 CD denotes 1
T3881 5802-5803 -COMMA- denotes ,
T3882 5804-5807 NN denotes A–D
T3883 5807-5808 -RRB- denotes )
T3884 5810-5818 JJ denotes Relative
T3885 5819-5821 TO denotes to
T3886 5822-5825 PRP-DOLLAR- denotes its
T3887 5826-5836 NN denotes expression
T3888 5837-5839 IN denotes in
T3889 5840-5845 JJ denotes naive
T3890 5846-5847 NN denotes T
T3891 5848-5853 NN denotes cells
T3892 5853-5854 -COMMA- denotes ,
T3893 5855-5863 NN denotes perforin
T3894 5864-5865 -LRB- denotes (
T3895 5865-5869 NN denotes Prf1
T3896 5869-5870 -RRB- denotes )
T3897 5871-5875 NN denotes mRNA
T3898 5876-5886 NN denotes expression
T3899 5887-5890 VB denotes did
T3900 5891-5894 RB denotes not
T3901 5895-5903 VB denotes increase
T3902 5904-5915 RB denotes appreciably
T3903 5916-5918 IN denotes at
T3904 5919-5922 NN denotes day
T3905 5923-5924 CD denotes 2
T3906 5925-5928 CC denotes but
T3907 5929-5935 VB denotes showed
T3908 5936-5937 DT denotes a
T3909 5938-5950 JJ denotes reproducible
T3910 5951-5959 NN denotes decrease
T3911 5960-5962 IN denotes at
T3912 5963-5966 NN denotes day
T3913 5967-5968 CD denotes 4
T3914 5968-5969 -COMMA- denotes ,
T3915 5970-5978 VB denotes followed
T3916 5979-5981 IN denotes by
T3917 5982-5988 JJ denotes robust
T3918 5989-6001 NN denotes reexpression
T3919 6002-6009 IN denotes between
T3920 6010-6014 NN denotes days
T3921 6015-6016 CD denotes 4
T3922 6017-6020 CC denotes and
T3923 6021-6022 CD denotes 8
T3924 6023-6024 -LRB- denotes (
T3925 6024-6028 NN denotes Fig.
T3926 6029-6030 CD denotes 1
T3927 6030-6031 -COMMA- denotes ,
T3928 6032-6035 NN denotes A–D
T3929 6035-6036 -RRB- denotes )
T3930 6038-6040 IN denotes In
T3931 6041-6049 NN denotes contrast
T3932 6049-6050 -COMMA- denotes ,
T3933 6051-6059 NN denotes granzyme
T3934 6060-6061 NN denotes B
T3935 6062-6063 -LRB- denotes (
T3936 6063-6067 NN denotes Gzmb
T3937 6067-6068 -RRB- denotes )
T3938 6069-6073 NN denotes mRNA
T3939 6074-6077 VB denotes was
T3940 6078-6081 JJ denotes low
T3941 6082-6084 CC denotes or
T3942 6085-6097 JJ denotes undetectable
T3943 6098-6100 IN denotes in
T3944 6101-6106 JJ denotes naive
T3945 6107-6108 NN denotes T
T3946 6109-6114 NN denotes cells
T3947 6115-6118 CC denotes but
T3948 6119-6122 VB denotes was
T3949 6123-6131 RB denotes strongly
T3950 6132-6144 VB denotes up-regulated
T3951 6145-6147 IN denotes by
T3952 6148-6151 NN denotes day
T3953 6152-6153 CD denotes 2
T3954 6154-6159 IN denotes after
T3955 6160-6171 NN denotes stimulation
T3956 6172-6175 CC denotes and
T3957 6176-6185 VB denotes increased
T3958 6186-6199 RB denotes progressively
T3959 6200-6205 IN denotes until
T3960 6206-6209 NN denotes day
T3961 6210-6211 CD denotes 6
T3962 6212-6213 -LRB- denotes (
T3963 6213-6217 NN denotes Fig.
T3964 6218-6219 CD denotes 1
T3965 6219-6220 -COMMA- denotes ,
T3966 6221-6222 NN denotes A
T3967 6223-6226 CC denotes and
T3968 6227-6228 NN denotes B
T3969 6228-6229 -RRB- denotes )
T3970 6229-6230 -COLON- denotes ;
T3971 6231-6240 RB denotes similarly
T3972 6240-6241 -COMMA- denotes ,
T3973 6242-6250 NN denotes granzyme
T3974 6251-6252 NN denotes B
T3975 6253-6260 NN denotes protein
T3976 6261-6264 VB denotes was
T3977 6265-6274 VB denotes expressed
T3978 6275-6277 IN denotes by
T3979 6278-6281 NN denotes day
T3980 6282-6283 CD denotes 4
T3981 6284-6287 CC denotes and
T3982 6288-6296 VB denotes remained
T3983 6297-6301 JJ denotes high
T3984 6302-6307 IN denotes until
T3985 6308-6311 NN denotes day
T3986 6312-6313 CD denotes 6
T3987 6314-6315 -LRB- denotes (
T3988 6315-6319 NN denotes Fig.
T3989 6320-6321 CD denotes 1
T3990 6322-6323 NN denotes E
T3991 6323-6324 -RRB- denotes )
T3992 6326-6328 IN denotes As
T3993 6329-6337 VB denotes expected
T3994 6337-6338 -COMMA- denotes ,
T3995 6339-6340 DT denotes a
T3996 6341-6346 JJ denotes small
T3997 6347-6355 NN denotes fraction
T3998 6356-6358 IN denotes of
T3999 6359-6364 JJ denotes naive
T4000 6365-6366 NN denotes T
T4001 6367-6372 NN denotes cells
T4002 6373-6382 VB denotes expressed
T4003 6383-6386 DT denotes the
T4004 6387-6396 NN denotes cytokines
T4005 6397-6402 NN denotes IFN-γ
T4006 6403-6406 CC denotes and
T4007 6407-6410 NN denotes TNF
T4008 6411-6413 IN denotes in
T4009 6414-6422 NN denotes response
T4010 6423-6425 TO denotes to
T4011 6426-6437 NN denotes stimulation
T4012 6437-6438 -COMMA- denotes ,
T4013 6439-6442 CC denotes and
T4014 6443-6447 DT denotes this
T4015 6448-6456 NN denotes capacity
T4016 6457-6466 VB denotes increased
T4017 6467-6480 RB denotes significantly
T4018 6481-6483 IN denotes in
T4019 6484-6498 VB denotes differentiated
T4020 6499-6504 NN denotes cells
T4021 6505-6506 -LRB- denotes (
T4022 6506-6510 NN denotes Fig.
T4023 6511-6512 CD denotes 1
T4024 6513-6514 NN denotes E
T4025 6514-6515 -COLON- denotes ;
T4026 6516-6519 VB denotes see
T4027 6520-6524 RB denotes also
T4028 6525-6529 NNP denotes Fig.
T4029 6530-6531 CD denotes 2
T4030 6532-6533 NN denotes A
T4031 6533-6534 -RRB- denotes )
T4032 7844-7846 PRP denotes We
T4033 7847-7856 VB denotes evaluated
T4034 7857-7874 JJ denotes antigen-dependent
T4035 7875-7884 JJ denotes cytolytic
T4036 7885-7893 NN denotes function
T4037 7894-7896 IN denotes in
T4038 7897-7898 DT denotes a
T4039 7899-7909 JJ denotes short-term
T4040 7910-7915 NN denotes assay
T4041 7916-7918 IN denotes in
T4042 7919-7924 WDT denotes which
T4043 7925-7931 NN denotes target
T4044 7932-7936 NN denotes cell
T4045 7937-7942 NN denotes death
T4046 7943-7946 VB denotes was
T4047 7947-7955 VB denotes measured
T4048 7956-7962 IN denotes within
T4049 7963-7964 CD denotes 2
T4050 7965-7966 NN denotes h
T4051 7967-7968 -LRB- denotes (
T4052 7968-7972 NN denotes Fig.
T4053 7973-7974 CD denotes 1
T4054 7975-7976 NN denotes F
T4055 7976-7977 -RRB- denotes )
T4056 7979-7981 IN denotes By
T4057 7982-7990 VB denotes limiting
T4058 7991-7994 DT denotes the
T4059 7995-8003 NN denotes duration
T4060 8004-8006 IN denotes of
T4061 8007-8010 NN denotes TCR
T4062 8011-8022 NN denotes stimulation
T4063 8022-8023 -COMMA- denotes ,
T4064 8024-8028 DT denotes this
T4065 8029-8037 NN denotes strategy
T4066 8038-8047 VB denotes minimizes
T4067 8048-8057 NN denotes cytolysis
T4068 8058-8067 JJ denotes secondary
T4069 8068-8070 TO denotes to
T4070 8071-8074 JJ denotes new
T4071 8075-8079 NN denotes gene
T4072 8080-8090 NN denotes expression
T4073 8091-8097 IN denotes during
T4074 8098-8101 DT denotes the
T4075 8102-8108 NN denotes period
T4076 8109-8111 IN denotes of
T4077 8112-8115 DT denotes the
T4078 8116-8121 NN denotes assay
T4079 8123-8128 JJ denotes Naive
T4080 8129-8130 NN denotes T
T4081 8131-8136 NN denotes cells
T4082 8137-8140 VB denotes did
T4083 8141-8144 RB denotes not
T4084 8145-8152 VB denotes display
T4085 8153-8164 JJ denotes significant
T4086 8165-8174 JJ denotes cytolytic
T4087 8175-8183 NN denotes function
T4088 8184-8186 IN denotes in
T4089 8187-8191 DT denotes this
T4090 8192-8202 JJ denotes short-term
T4091 8203-8208 NN denotes assay
T4092 8209-8210 -LRB- denotes (
T4093 8210-8221 JJ denotes unpublished
T4094 8222-8226 NN denotes data
T4095 8226-8227 -RRB- denotes )
T4096 8227-8228 -COMMA- denotes ,
T4097 8229-8233 RB denotes most
T4098 8234-8240 RB denotes likely
T4099 8241-8248 IN denotes because
T4100 8249-8253 PRP denotes they
T4101 8254-8261 VB denotes express
T4102 8262-8270 JJ denotes immature
T4103 8271-8272 -LRB- denotes (
T4104 8272-8283 JJ denotes unprocessed
T4105 8283-8284 -RRB- denotes )
T4106 8285-8290 NN denotes forms
T4107 8291-8293 IN denotes of
T4108 8294-8302 NN denotes perforin
T4109 8303-8306 CC denotes and
T4110 8307-8311 VB denotes lack
T4111 8312-8315 DT denotes the
T4112 8316-8324 NN denotes capacity
T4113 8325-8327 TO denotes to
T4114 8328-8339 VB denotes degranulate
T4115 8340-8341 -LRB- denotes (
T4116 8341-8343 CD denotes 18
T4117 8343-8344 -COMMA- denotes ,
T4118 8345-8347 CD denotes 19
T4119 8347-8348 -RRB- denotes )
T4120 8350-8354 RB denotes Even
T4121 8355-8360 IN denotes after
T4122 8361-8371 NN denotes activation
T4123 8372-8375 IN denotes for
T4124 8376-8377 CD denotes 2
T4125 8378-8380 CC denotes or
T4126 8381-8382 CD denotes 4
T4127 8383-8384 NN denotes d
T4128 8384-8385 -COMMA- denotes ,
T4129 8386-8389 DT denotes the
T4130 8390-8395 NN denotes cells
T4131 8396-8402 VB denotes showed
T4132 8403-8407 JJ denotes poor
T4133 8408-8417 JJ denotes cytolytic
T4134 8418-8426 NN denotes activity
T4135 8427-8428 -LRB- denotes (
T4136 8428-8432 NN denotes Fig.
T4137 8433-8434 CD denotes 1
T4138 8435-8436 NN denotes F
T4139 8436-8437 -RRB- denotes )
T4140 8437-8438 -COMMA- denotes ,
T4141 8439-8441 IN denotes in
T4142 8442-8450 JJ denotes striking
T4143 8451-8459 NN denotes contrast
T4144 8460-8462 TO denotes to
T4145 8463-8468 PRP-DOLLAR- denotes their
T4146 8469-8477 NN denotes capacity
T4147 8478-8481 IN denotes for
T4148 8482-8491 JJ denotes efficient
T4149 8492-8500 NN denotes cytokine
T4150 8501-8511 NN denotes production
T4151 8512-8513 -LRB- denotes (
T4152 8513-8517 NN denotes Fig.
T4153 8518-8519 CD denotes 1
T4154 8520-8521 NN denotes E
T4155 8521-8522 -RRB- denotes )
T4156 8524-8528 JJ denotes Only
T4157 8529-8534 NN denotes cells
T4158 8535-8543 VB denotes cultured
T4159 8544-8549 IN denotes until
T4160 8550-8553 NN denotes day
T4161 8554-8555 CD denotes 6
T4162 8556-8565 VB denotes displayed
T4163 8566-8572 JJ denotes robust
T4164 8573-8585 NN denotes cytotoxicity
T4165 8585-8586 -COMMA- denotes ,
T4166 8587-8589 IN denotes as
T4167 8590-8596 VB denotes judged
T4168 8597-8599 IN denotes by
T4169 8600-8605 PRP-DOLLAR- denotes their
T4170 8606-8613 NN denotes ability
T4171 8614-8616 TO denotes to
T4172 8617-8623 VB denotes induce
T4173 8624-8633 NN denotes apoptosis
T4174 8634-8636 IN denotes in
T4175 8637-8638 DT denotes a
T4176 8639-8644 JJ denotes large
T4177 8645-8651 NN denotes number
T4178 8652-8654 IN denotes of
T4179 8655-8661 NN denotes target
T4180 8662-8667 NN denotes cells
T4181 8668-8669 -LRB- denotes (
T4182 8669-8673 NN denotes Fig.
T4183 8674-8675 CD denotes 1
T4184 8676-8677 NN denotes F
T4185 8677-8678 -RRB- denotes )
T4186 8680-8685 DT denotes These
T4187 8686-8693 NN denotes results
T4188 8694-8698 VB denotes show
T4189 8699-8703 IN denotes that
T4190 8704-8709 IN denotes after
T4191 8710-8711 DT denotes a
T4192 8712-8718 JJ denotes strong
T4193 8719-8726 NN denotes priming
T4194 8727-8735 NN denotes stimulus
T4195 8736-8743 IN denotes through
T4196 8744-8748 NN denotes TCRs
T4197 8749-8752 CC denotes and
T4198 8753-8767 JJ denotes co-stimulatory
T4199 8768-8777 NN denotes receptors
T4200 8778-8780 FW denotes in
T4201 8781-8786 FW denotes vitro
T4202 8786-8787 -COMMA- denotes ,
T4203 8788-8796 NN denotes granzyme
T4204 8797-8798 NN denotes B
T4205 8799-8809 NN denotes expression
T4206 8810-8813 CC denotes and
T4207 8814-8817 DT denotes the
T4208 8818-8825 NN denotes ability
T4209 8826-8828 TO denotes to
T4210 8829-8836 VB denotes produce
T4211 8837-8845 NN denotes effector
T4212 8846-8855 NN denotes cytokines
T4213 8856-8859 VB denotes are
T4214 8860-8870 VB denotes programmed
T4215 8871-8876 RB denotes early
T4216 8876-8877 -COMMA- denotes ,
T4217 8878-8885 IN denotes whereas
T4218 8886-8894 NN denotes perforin
T4219 8895-8905 NN denotes expression
T4220 8906-8909 CC denotes and
T4221 8910-8919 JJ denotes cytolytic
T4222 8920-8928 NN denotes function
T4223 8929-8932 VB denotes are
T4224 8933-8940 VB denotes induced
T4225 8941-8946 RB denotes later
T4226 8946-8947 -COMMA- denotes ,
T4227 8948-8954 IN denotes during
T4228 8955-8958 DT denotes the
T4229 8959-8964 NN denotes phase
T4230 8965-8967 IN denotes of
T4231 8968-8974 JJ denotes clonal
T4232 8975-8984 NN denotes expansion
T4233 8985-8987 IN denotes in
T4234 8988-8992 NN denotes IL-2
T4235 8994-9003 RB denotes Therefore
T4236 9003-9004 -COMMA- denotes ,
T4237 9005-9008 DT denotes the
T4238 9009-9012 CD denotes two
T4239 9013-9018 JJ denotes major
T4240 9019-9027 NN denotes effector
T4241 9028-9037 NN denotes functions
T4242 9038-9040 IN denotes of
T4243 9041-9044 NN denotes CTL
T4244 9044-9045 -COMMA- denotes ,
T4245 9046-9054 NN denotes cytokine
T4246 9055-9065 NN denotes production
T4247 9066-9069 CC denotes and
T4248 9070-9079 JJ denotes cytolytic
T4249 9080-9088 NN denotes activity
T4250 9088-9089 -COMMA- denotes ,
T4251 9090-9093 VB denotes are
T4252 9094-9097 RB denotes not
T4253 9098-9111 RB denotes intrinsically
T4254 9112-9123 VB denotes coregulated
T5120 9126-9134 JJ denotes Distinct
T5121 9135-9143 NN denotes kinetics
T5122 9144-9146 IN denotes of
T5123 9147-9152 NN denotes T-bet
T5124 9153-9156 CC denotes and
T5125 9157-9162 NN denotes Eomes
T5126 9163-9173 NN denotes expression
T5127 9174-9180 IN denotes during
T5128 9181-9184 NN denotes CTL
T5129 9185-9200 NN denotes differentiation
T5130 9201-9204 DT denotes The
T5131 9205-9210 NN denotes T-box
T5132 9211-9224 NN denotes transcription
T5133 9225-9232 NN denotes factors
T5134 9233-9238 NN denotes T-bet
T5135 9239-9242 CC denotes and
T5136 9243-9248 NN denotes Eomes
T5137 9249-9253 VB denotes have
T5138 9254-9258 VB denotes been
T5139 9259-9265 VB denotes linked
T5140 9266-9268 TO denotes to
T5141 9269-9272 DT denotes the
T5142 9273-9283 NN denotes regulation
T5143 9284-9286 IN denotes of
T5144 9287-9292 NN denotes genes
T5145 9293-9301 VB denotes encoding
T5146 9302-9310 NN denotes effector
T5147 9311-9320 NN denotes cytokines
T5148 9321-9322 -LRB- denotes (
T5149 9322-9326 FW denotes e.g.
T5150 9326-9327 -COMMA- denotes ,
T5151 9328-9332 NN denotes Ifng
T5152 9332-9333 -RRB- denotes )
T5153 9334-9337 CC denotes and
T5154 9338-9343 NN denotes genes
T5155 9344-9353 JJ denotes important
T5156 9354-9357 IN denotes for
T5157 9358-9367 JJ denotes cytolytic
T5158 9368-9376 NN denotes function
T5159 9377-9378 -LRB- denotes (
T5160 9378-9382 FW denotes e.g.
T5161 9382-9383 -COMMA- denotes ,
T5162 9384-9388 NN denotes Prf1
T5163 9389-9392 CC denotes and
T5164 9393-9397 NN denotes GzmB
T5165 9397-9398 -RRB- denotes )
T5166 9399-9400 -LRB- denotes (
T5167 9400-9402 CD denotes 20
T5168 9402-9403 -RRB- denotes )
T5169 9405-9407 PRP denotes We
T5170 9408-9420 VB denotes investigated
T5171 9421-9424 DT denotes the
T5172 9425-9433 NN denotes kinetics
T5173 9434-9436 IN denotes of
T5174 9437-9447 NN denotes expression
T5175 9448-9450 IN denotes of
T5176 9451-9456 DT denotes these
T5177 9457-9470 NN denotes transcription
T5178 9471-9478 NN denotes factors
T5179 9479-9481 IN denotes in
T5180 9482-9485 PRP-DOLLAR- denotes our
T5181 9486-9488 FW denotes in
T5182 9489-9494 FW denotes vitro
T5183 9495-9503 NN denotes cultures
T5184 9504-9505 -LRB- denotes (
T5185 9505-9509 NNP denotes Fig.
T5186 9510-9511 CD denotes 1
T5187 9511-9512 -COMMA- denotes ,
T5188 9513-9516 NN denotes A–D
T5189 9516-9517 -RRB- denotes )
T5190 9519-9524 NN denotes T-bet
T5191 9525-9529 NN denotes mRNA
T5192 9530-9533 CC denotes and
T5193 9534-9541 NN denotes protein
T5194 9542-9546 VB denotes were
T5195 9547-9550 RB denotes not
T5196 9551-9561 JJ denotes detectable
T5197 9562-9564 IN denotes in
T5198 9565-9570 JJ denotes naive
T5199 9571-9575 JJ denotes CD8+
T5200 9576-9577 NN denotes T
T5201 9578-9583 NN denotes cells
T5202 9583-9584 -COMMA- denotes ,
T5203 9585-9588 CC denotes but
T5204 9589-9593 VB denotes were
T5205 9594-9602 RB denotes strongly
T5206 9603-9610 VB denotes induced
T5207 9611-9615 IN denotes upon
T5208 9616-9619 NN denotes TCR
T5209 9620-9627 NN denotes priming
T5210 9628-9629 -LRB- denotes (
T5211 9629-9632 NN denotes day
T5212 9633-9634 CD denotes 2
T5213 9634-9635 -RRB- denotes )
T5214 9636-9639 CC denotes and
T5215 9640-9648 VB denotes remained
T5216 9649-9658 VB denotes expressed
T5217 9659-9666 IN denotes through
T5218 9667-9670 NN denotes day
T5219 9671-9672 CD denotes 6
T5220 9673-9675 IN denotes of
T5221 9676-9691 NN denotes differentiation
T5222 9692-9693 -LRB- denotes (
T5223 9693-9697 NN denotes Fig.
T5224 9698-9699 CD denotes 1
T5225 9699-9700 -COMMA- denotes ,
T5226 9701-9702 NN denotes A
T5227 9703-9706 CC denotes and
T5228 9707-9708 NN denotes C
T5229 9708-9709 -COLON- denotes ;
T5230 9710-9720 VB denotes quantified
T5231 9721-9723 IN denotes in
T5232 9724-9728 NNP denotes Fig.
T5233 9729-9730 CD denotes 1
T5234 9730-9731 -COMMA- denotes ,
T5235 9732-9733 NN denotes B
T5236 9734-9737 CC denotes and
T5237 9738-9739 NN denotes D
T5238 9739-9740 -RRB- denotes )
T5239 9742-9744 IN denotes In
T5240 9745-9753 NN denotes contrast
T5241 9753-9754 -COMMA- denotes ,
T5242 9755-9760 NN denotes Eomes
T5243 9761-9771 NN denotes expression
T5244 9772-9775 VB denotes was
T5245 9776-9779 JJ denotes low
T5246 9780-9782 CC denotes or
T5247 9783-9795 JJ denotes undetectable
T5248 9796-9798 IN denotes at
T5249 9799-9803 CC denotes both
T5250 9804-9807 DT denotes the
T5251 9808-9812 NN denotes mRNA
T5252 9813-9816 CC denotes and
T5253 9817-9824 NN denotes protein
T5254 9825-9831 NN denotes levels
T5255 9832-9834 IN denotes in
T5256 9835-9840 JJ denotes naive
T5257 9841-9845 JJ denotes CD8+
T5258 9846-9847 NN denotes T
T5259 9848-9853 NN denotes cells
T5260 9853-9854 -COMMA- denotes ,
T5261 9855-9858 CC denotes and
T5262 9859-9862 NN denotes TCR
T5263 9863-9870 NN denotes priming
T5264 9871-9873 IN denotes in
T5265 9874-9881 NN denotes culture
T5266 9882-9885 VB denotes had
T5267 9886-9890 RB denotes only
T5268 9891-9892 DT denotes a
T5269 9893-9899 JJ denotes modest
T5270 9900-9906 NN denotes effect
T5271 9907-9909 IN denotes on
T5272 9910-9913 PRP-DOLLAR- denotes its
T5273 9914-9924 NN denotes expression
T5274 9925-9927 IN denotes at
T5275 9928-9931 NN denotes day
T5276 9932-9933 CD denotes 2
T5277 9934-9935 -LRB- denotes (
T5278 9935-9939 NN denotes Fig.
T5279 9940-9941 CD denotes 1
T5280 9941-9942 -COMMA- denotes ,
T5281 9943-9944 NN denotes A
T5282 9945-9948 CC denotes and
T5283 9949-9950 NN denotes C
T5284 9950-9951 -RRB- denotes )
T5285 9953-9959 JJ denotes Strong
T5286 9960-9969 NN denotes induction
T5287 9970-9972 IN denotes of
T5288 9973-9978 NN denotes Eomes
T5289 9979-9983 NN denotes mRNA
T5290 9984-9987 CC denotes and
T5291 9988-9995 NN denotes protein
T5292 9996-9999 VB denotes was
T5293 10000-10004 RB denotes only
T5294 10005-10013 VB denotes observed
T5295 10014-10016 IN denotes at
T5296 10017-10020 NN denotes day
T5297 10021-10022 CD denotes 4
T5298 10023-10026 CC denotes and
T5299 10027-10032 RB denotes later
T5300 10033-10034 -LRB- denotes (
T5301 10034-10038 NNP denotes Fig.
T5302 10039-10040 CD denotes 1
T5303 10040-10041 -COMMA- denotes ,
T5304 10042-10043 NN denotes A
T5305 10044-10047 CC denotes and
T5306 10048-10049 NN denotes C
T5307 10049-10050 -RRB- denotes )
T5308 10052-10057 NN denotes T-bet
T5309 10058-10062 NN denotes mRNA
T5310 10063-10073 NN denotes expression
T5311 10074-10082 RB denotes slightly
T5312 10083-10091 VB denotes preceded
T5313 10092-10095 DT denotes the
T5314 10096-10106 NN denotes expression
T5315 10107-10109 IN denotes of
T5316 10110-10114 NN denotes GzmB
T5317 10115-10119 NN denotes mRNA
T5318 10119-10120 -COLON- denotes ;
T5319 10121-10130 RB denotes similarly
T5320 10130-10131 -COMMA- denotes ,
T5321 10132-10137 NN denotes Eomes
T5322 10138-10142 NN denotes mRNA
T5323 10143-10146 CC denotes and
T5324 10147-10154 NN denotes protein
T5325 10155-10159 VB denotes were
T5326 10160-10169 VB denotes expressed
T5327 10170-10172 CD denotes ∼1
T5328 10173-10174 NN denotes d
T5329 10175-10180 RB denotes ahead
T5330 10181-10183 IN denotes of
T5331 10184-10187 DT denotes the
T5332 10188-10200 NN denotes reexpression
T5333 10201-10203 IN denotes of
T5334 10204-10212 NN denotes perforin
T5335 10213-10217 NN denotes mRNA
T5336 10218-10221 CC denotes and
T5337 10222-10229 NN denotes protein
T5338 10229-10230 -COMMA- denotes ,
T5339 10231-10243 RB denotes respectively
T5340 10244-10245 -LRB- denotes (
T5341 10245-10249 NNP denotes Fig.
T5342 10250-10251 CD denotes 1
T5343 10251-10252 -COMMA- denotes ,
T5344 10253-10254 NN denotes B
T5345 10255-10258 CC denotes and
T5346 10259-10260 NN denotes D
T5347 10260-10261 -RRB- denotes )
T5348 10263-10267 DT denotes This
T5349 10268-10276 JJ denotes detailed
T5350 10277-10284 JJ denotes kinetic
T5351 10285-10293 NN denotes analysis
T5352 10294-10303 VB denotes suggested
T5353 10304-10308 IN denotes that
T5354 10308-10309 -COMMA- denotes ,
T5355 10310-10315 IN denotes under
T5356 10316-10319 PRP-DOLLAR- denotes our
T5357 10320-10327 NN denotes culture
T5358 10328-10338 NN denotes conditions
T5359 10338-10339 -COMMA- denotes ,
T5360 10340-10345 NN denotes T-bet
T5361 10346-10349 CC denotes and
T5362 10350-10355 NN denotes Eomes
T5363 10356-10366 VB denotes contribute
T5364 10367-10369 TO denotes to
T5365 10370-10378 JJ denotes distinct
T5366 10379-10386 NN denotes aspects
T5367 10387-10389 IN denotes of
T5368 10390-10394 NN denotes gene
T5369 10395-10408 NN denotes transcription
T5370 10409-10415 IN denotes during
T5371 10416-10419 NN denotes CTL
T5372 10420-10435 NN denotes differentiation
T5373 10437-10442 NN denotes T-bet
T5374 10443-10445 VB denotes is
T5375 10446-10454 VB denotes required
T5376 10455-10460 RB denotes early
T5377 10461-10464 IN denotes for
T5378 10465-10470 NN denotes IFN-γ
T5379 10471-10481 NN denotes production
T5380 10481-10482 -COMMA- denotes ,
T5381 10483-10486 CC denotes and
T5382 10487-10490 PRP-DOLLAR- denotes our
T5383 10491-10495 NN denotes data
T5384 10496-10505 VB denotes suggested
T5385 10506-10510 IN denotes that
T5386 10511-10516 NN denotes Eomes
T5387 10517-10522 MD denotes might
T5388 10523-10526 RB denotes not
T5389 10527-10535 VB denotes function
T5390 10536-10542 IN denotes during
T5391 10543-10547 DT denotes this
T5392 10548-10553 JJ denotes early
T5393 10554-10560 NN denotes period
T5394 10561-10564 CC denotes but
T5395 10565-10571 RB denotes rather
T5396 10572-10577 MD denotes might
T5397 10578-10588 VB denotes contribute
T5398 10589-10594 RB denotes later
T5399 10595-10597 TO denotes to
T5400 10598-10601 DT denotes the
T5401 10602-10609 NN denotes control
T5402 10610-10612 IN denotes of
T5403 10613-10621 NN denotes perforin
T5404 10622-10632 NN denotes expression
T5405 10634-10637 PRP-DOLLAR- denotes Our
T5406 10638-10642 NN denotes data
T5407 10643-10649 VB denotes seemed
T5408 10650-10654 RB denotes most
T5409 10655-10665 JJ denotes consistent
T5410 10666-10670 IN denotes with
T5411 10671-10672 DT denotes a
T5412 10673-10678 NN denotes model
T5413 10679-10681 IN denotes in
T5414 10682-10687 WDT denotes which
T5415 10688-10691 NN denotes TCR
T5416 10692-10699 NN denotes signals
T5417 10700-10706 VB denotes induce
T5418 10707-10712 NN denotes T-bet
T5419 10712-10713 -COMMA- denotes ,
T5420 10714-10719 WDT denotes which
T5421 10720-10722 IN denotes in
T5422 10723-10727 NN denotes turn
T5423 10728-10735 VB denotes induces
T5424 10736-10741 NN denotes IFN-γ
T5425 10742-10743 -LRB- denotes (
T5426 10743-10745 CD denotes 17
T5427 10745-10746 -RRB- denotes )
T5428 10747-10750 CC denotes and
T5429 10751-10759 RB denotes possibly
T5430 10760-10768 NN denotes granzyme
T5431 10769-10770 NN denotes B
T5432 10770-10771 -COLON- denotes ;
T5433 10772-10784 RB denotes subsequently
T5434 10784-10785 -COMMA- denotes ,
T5435 10786-10791 NN denotes Eomes
T5436 10792-10794 VB denotes is
T5437 10795-10802 VB denotes induced
T5438 10803-10809 IN denotes during
T5439 10810-10813 DT denotes the
T5440 10814-10820 NN denotes period
T5441 10821-10823 IN denotes of
T5442 10824-10830 JJ denotes clonal
T5443 10831-10840 NN denotes expansion
T5444 10841-10843 IN denotes in
T5445 10844-10848 NN denotes IL-2
T5446 10849-10852 CC denotes and
T5447 10853-10862 VB denotes activates
T5448 10863-10871 NN denotes perforin
T5449 10872-10882 NN denotes expression
T6493 10885-10893 NN denotes Perforin
T6494 10894-10897 CC denotes and
T6495 10898-10906 NN denotes granzyme
T6496 10907-10908 NN denotes B
T6497 10909-10919 NN denotes expression
T6498 10920-10923 VB denotes are
T6499 10924-10927 RB denotes not
T6500 10928-10939 RB denotes appreciably
T6501 10940-10949 VB denotes regulated
T6502 10950-10952 IN denotes by
T6503 10953-10958 NN denotes T-bet
T6504 10959-10961 TO denotes To
T6505 10962-10966 VB denotes test
T6506 10967-10970 DT denotes the
T6507 10971-10976 NN denotes model
T6508 10977-10985 VB denotes outlined
T6509 10986-10988 IN denotes in
T6510 10989-10992 DT denotes the
T6511 10993-11001 JJ denotes previous
T6512 11002-11011 NN denotes paragraph
T6513 11012-11020 RB denotes directly
T6514 11020-11021 -COMMA- denotes ,
T6515 11022-11024 PRP denotes we
T6516 11025-11033 VB denotes compared
T6517 11034-11037 DT denotes the
T6518 11038-11048 NN denotes expression
T6519 11049-11051 IN denotes of
T6520 11052-11057 NN denotes IFN-γ
T6521 11057-11058 -COMMA- denotes ,
T6522 11059-11067 NN denotes perforin
T6523 11067-11068 -COMMA- denotes ,
T6524 11069-11072 CC denotes and
T6525 11073-11081 NN denotes granzyme
T6526 11082-11083 NN denotes B
T6527 11084-11086 IN denotes in
T6528 11087-11091 JJ denotes CD8+
T6529 11092-11093 NN denotes T
T6530 11094-11099 NN denotes cells
T6531 11100-11104 IN denotes from
T6532 11105-11107 NN denotes WT
T6533 11108-11111 CC denotes and
T6534 11112-11117 NN denotes Tbx21
T6535 11118-11119 -LRB- denotes (
T6536 11119-11124 NN denotes T-bet
T6537 11124-11125 -RRB- denotes )
T6538 11125-11135 JJ denotes -deficient
T6539 11136-11140 NN denotes mice
T6540 11142-11144 IN denotes As
T6541 11145-11153 VB denotes expected
T6542 11154-11155 -LRB- denotes (
T6543 11155-11157 CD denotes 17
T6544 11157-11158 -COMMA- denotes ,
T6545 11159-11161 CD denotes 21
T6546 11161-11162 -RRB- denotes )
T6547 11162-11163 -COMMA- denotes ,
T6548 11164-11169 JJ denotes naive
T6549 11170-11178 NN denotes Tbx21−/−
T6550 11179-11183 JJ denotes CD8+
T6551 11184-11185 NN denotes T
T6552 11186-11191 NN denotes cells
T6553 11192-11200 VB denotes produced
T6554 11201-11206 NN denotes IFN-γ
T6555 11207-11213 RB denotes poorly
T6556 11214-11218 IN denotes upon
T6557 11219-11229 NN denotes activation
T6558 11230-11231 -LRB- denotes (
T6559 11231-11235 NN denotes Fig.
T6560 11236-11237 CD denotes 2
T6561 11238-11239 NN denotes A
T6562 11239-11240 -RRB- denotes )
T6563 11242-11249 RB denotes Notably
T6564 11249-11250 -COMMA- denotes ,
T6565 11251-11255 DT denotes this
T6566 11256-11267 JJ denotes deleterious
T6567 11268-11274 NN denotes effect
T6568 11275-11277 IN denotes of
T6569 11278-11283 NN denotes T-bet
T6570 11284-11294 NN denotes deficiency
T6571 11295-11298 VB denotes was
T6572 11299-11303 RB denotes only
T6573 11304-11312 VB denotes observed
T6574 11313-11315 IN denotes in
T6575 11316-11331 VB denotes differentiating
T6576 11332-11336 JJ denotes CD8+
T6577 11337-11338 NN denotes T
T6578 11339-11344 NN denotes cells
T6579 11345-11350 IN denotes until
T6580 11351-11354 NN denotes day
T6581 11355-11356 CD denotes 4
T6582 11357-11359 IN denotes of
T6583 11360-11367 NN denotes culture
T6584 11368-11371 CC denotes but
T6585 11372-11375 VB denotes was
T6586 11376-11382 RB denotes almost
T6587 11383-11393 RB denotes completely
T6588 11394-11403 VB denotes mitigated
T6589 11404-11406 IN denotes by
T6590 11407-11410 NN denotes day
T6591 11411-11412 CD denotes 6
T6592 11413-11414 -LRB- denotes (
T6593 11414-11418 NN denotes Fig.
T6594 11419-11420 CD denotes 2
T6595 11421-11422 NN denotes A
T6596 11422-11423 -RRB- denotes )
T6597 11425-11429 DT denotes This
T6598 11430-11434 RB denotes most
T6599 11435-11441 JJ denotes likely
T6600 11442-11451 VB denotes reflected
T6601 11452-11464 NN denotes compensation
T6602 11465-11467 IN denotes by
T6603 11468-11473 NN denotes Eomes
T6604 11473-11474 -COMMA- denotes ,
T6605 11475-11480 WDT denotes which
T6606 11481-11484 VB denotes was
T6607 11485-11493 RB denotes strongly
T6608 11494-11501 VB denotes induced
T6609 11502-11509 IN denotes between
T6610 11510-11514 NN denotes days
T6611 11515-11516 CD denotes 4
T6612 11517-11520 CC denotes and
T6613 11521-11522 CD denotes 6
T6614 11523-11524 -LRB- denotes (
T6615 11524-11528 NN denotes Fig.
T6616 11529-11530 CD denotes 1
T6617 11530-11531 -RRB- denotes )
T6618 11533-11535 IN denotes In
T6619 11536-11544 NN denotes contrast
T6620 11544-11545 -COMMA- denotes ,
T6621 11546-11561 JJ denotes T-bet–deficient
T6622 11562-11563 NN denotes T
T6623 11564-11569 NN denotes cells
T6624 11570-11578 VB denotes cultured
T6625 11579-11582 IN denotes for
T6626 11583-11584 CD denotes 6
T6627 11585-11586 NN denotes d
T6628 11587-11593 VB denotes showed
T6629 11594-11596 DT denotes no
T6630 11597-11603 NN denotes defect
T6631 11604-11606 IN denotes in
T6632 11607-11615 NN denotes perforin
T6633 11616-11620 NN denotes mRNA
T6634 11621-11631 NN denotes expression
T6635 11632-11633 -LRB- denotes (
T6636 11633-11637 NN denotes Fig.
T6637 11638-11639 CD denotes 2
T6638 11640-11641 NN denotes B
T6639 11641-11642 -COMMA- denotes ,
T6640 11643-11650 VB denotes compare
T6641 11651-11656 NN denotes lanes
T6642 11657-11658 CD denotes 1
T6643 11659-11662 CC denotes and
T6644 11663-11664 CD denotes 4
T6645 11664-11665 -RRB- denotes )
T6646 11667-11669 PRP denotes We
T6647 11670-11682 RB denotes consistently
T6648 11683-11691 VB denotes observed
T6649 11692-11693 DT denotes a
T6650 11694-11700 JJ denotes modest
T6651 11701-11710 NN denotes reduction
T6652 11711-11713 IN denotes in
T6653 11714-11718 NN denotes GzmB
T6654 11719-11723 NN denotes mRNA
T6655 11724-11726 IN denotes in
T6656 11727-11742 JJ denotes T-bet–deficient
T6657 11743-11744 NN denotes T
T6658 11745-11750 NN denotes cells
T6659 11751-11752 -LRB- denotes (
T6660 11752-11756 NN denotes Fig.
T6661 11757-11758 CD denotes 2
T6662 11759-11760 NN denotes B
T6663 11760-11761 -COMMA- denotes ,
T6664 11762-11769 VB denotes compare
T6665 11770-11775 NN denotes lanes
T6666 11776-11777 CD denotes 1
T6667 11778-11781 CC denotes and
T6668 11782-11783 CD denotes 4
T6669 11783-11784 -RRB- denotes )
T6670 11784-11785 -COMMA- denotes ,
T6671 11786-11791 WDT denotes which
T6672 11792-11795 VB denotes did
T6673 11796-11799 RB denotes not
T6674 11800-11809 VB denotes translate
T6675 11810-11814 IN denotes into
T6676 11815-11816 DT denotes a
T6677 11817-11825 NN denotes decrease
T6678 11826-11828 IN denotes in
T6679 11829-11839 NN denotes expression
T6680 11840-11842 IN denotes of
T6681 11843-11851 NN denotes granzyme
T6682 11852-11853 NN denotes B
T6683 11854-11861 NN denotes protein
T6684 11862-11863 -LRB- denotes (
T6685 11863-11867 NN denotes Fig.
T6686 11868-11869 CD denotes 2
T6687 11870-11871 NN denotes C
T6688 11871-11872 -RRB- denotes )
T6689 13130-13132 TO denotes To
T6690 13133-13140 VB denotes examine
T6691 13141-13144 DT denotes the
T6692 13145-13149 NN denotes role
T6693 13150-13152 IN denotes of
T6694 13153-13158 NN denotes Eomes
T6695 13158-13159 -COMMA- denotes ,
T6696 13160-13162 PRP denotes we
T6697 13163-13173 VB denotes transduced
T6698 13174-13179 JJ denotes naive
T6699 13180-13184 JJ denotes CD8+
T6700 13185-13186 NN denotes T
T6701 13187-13192 NN denotes cells
T6702 13193-13197 IN denotes from
T6703 13198-13200 NN denotes WT
T6704 13201-13204 CC denotes and
T6705 13205-13213 NN denotes Tbx21−/−
T6706 13214-13218 NN denotes mice
T6707 13219-13223 IN denotes with
T6708 13224-13236 NN denotes retroviruses
T6709 13237-13247 VB denotes containing
T6710 13248-13256 JJ denotes internal
T6711 13257-13265 NN denotes ribosome
T6712 13266-13271 NN denotes entry
T6713 13272-13276 NN denotes site
T6714 13277-13278 -LRB- denotes (
T6715 13278-13282 NN denotes IRES
T6716 13282-13283 -RRB- denotes )
T6717 13283-13287 NN denotes –GFP
T6718 13288-13292 WDT denotes that
T6719 13293-13297 VB denotes were
T6720 13298-13304 CC denotes either
T6721 13305-13310 JJ denotes empty
T6722 13311-13313 CC denotes or
T6723 13314-13321 VB denotes encoded
T6724 13322-13323 DT denotes a
T6725 13324-13332 RB denotes strongly
T6726 13333-13348 VB denotes transactivating
T6727 13349-13356 NN denotes version
T6728 13357-13359 IN denotes of
T6729 13360-13365 NN denotes Eomes
T6730 13366-13367 -LRB- denotes (
T6731 13367-13374 NN denotes Eo-VP16
T6732 13374-13375 -RRB- denotes )
T6733 13376-13377 -LRB- denotes (
T6734 13377-13378 CD denotes 8
T6735 13378-13379 -RRB- denotes )
T6736 13379-13380 -COMMA- denotes ,
T6737 13381-13384 CC denotes and
T6738 13385-13393 VB denotes expanded
T6739 13394-13398 PRP denotes them
T6740 13399-13402 IN denotes for
T6741 13403-13404 CD denotes 6
T6742 13405-13406 NN denotes d
T6743 13407-13412 IN denotes under
T6744 13413-13416 PRP-DOLLAR- denotes our
T6745 13417-13424 NN denotes culture
T6746 13425-13435 NN denotes conditions
T6747 13437-13444 NN denotes Eo-VP16
T6748 13444-13445 -COMMA- denotes ,
T6749 13446-13449 CC denotes but
T6750 13450-13453 RB denotes not
T6751 13454-13457 DT denotes the
T6752 13458-13463 JJ denotes empty
T6753 13464-13467 NN denotes GFP
T6754 13468-13478 NN denotes retrovirus
T6755 13478-13479 -COMMA- denotes ,
T6756 13480-13489 VB denotes increased
T6757 13490-13498 NN denotes perforin
T6758 13499-13509 NN denotes expression
T6759 13510-13512 IN denotes in
T6760 13513-13517 CC denotes both
T6761 13518-13520 NN denotes WT
T6762 13521-13524 CC denotes and
T6763 13525-13540 JJ denotes T-bet–deficient
T6764 13541-13545 JJ denotes CD8+
T6765 13546-13547 NN denotes T
T6766 13548-13553 NN denotes cells
T6767 13554-13555 -LRB- denotes (
T6768 13555-13559 NN denotes Fig.
T6769 13560-13561 CD denotes 2
T6770 13562-13563 NN denotes B
T6771 13563-13564 -COMMA- denotes ,
T6772 13565-13570 NN denotes lanes
T6773 13571-13572 CD denotes 2
T6774 13572-13573 -COMMA- denotes ,
T6775 13574-13575 CD denotes 3
T6776 13575-13576 -COMMA- denotes ,
T6777 13577-13578 CD denotes 5
T6778 13578-13579 -COMMA- denotes ,
T6779 13580-13583 CC denotes and
T6780 13584-13585 CD denotes 6
T6781 13585-13586 -RRB- denotes )
T6782 13588-13590 IN denotes As
T6783 13591-13599 VB denotes expected
T6784 13599-13600 -COMMA- denotes ,
T6785 13601-13608 NN denotes Eo-VP16
T6786 13609-13613 RB denotes also
T6787 13614-13621 VB denotes rescued
T6788 13622-13625 DT denotes the
T6789 13626-13631 JJ denotes early
T6790 13632-13638 NN denotes defect
T6791 13639-13641 IN denotes in
T6792 13642-13647 NN denotes IFN-γ
T6793 13648-13658 NN denotes production
T6794 13659-13667 VB denotes observed
T6795 13668-13670 IN denotes in
T6796 13671-13686 JJ denotes T-bet–deficient
T6797 13687-13691 JJ denotes CD8+
T6798 13692-13693 NN denotes T
T6799 13694-13699 NN denotes cells
T6800 13700-13701 -LRB- denotes (
T6801 13701-13705 NN denotes Fig.
T6802 13706-13707 CD denotes 2
T6803 13708-13709 NN denotes D
T6804 13709-13710 -RRB- denotes )
T6805 13712-13719 RB denotes However
T6806 13719-13720 -COMMA- denotes ,
T6807 13721-13728 NN denotes Eo-VP16
T6808 13729-13732 VB denotes did
T6809 13733-13736 RB denotes not
T6810 13737-13743 VB denotes induce
T6811 13744-13748 NN denotes GzmB
T6812 13749-13753 NN denotes mRNA
T6813 13754-13764 NN denotes expression
T6814 13765-13767 IN denotes in
T6815 13768-13774 CC denotes either
T6816 13775-13777 NN denotes WT
T6817 13778-13780 CC denotes or
T6818 13781-13796 JJ denotes T-bet–deficient
T6819 13797-13802 NN denotes cells
T6820 13802-13803 -COLON- denotes ;
T6821 13804-13808 RB denotes thus
T6822 13808-13809 -COMMA- denotes ,
T6823 13810-13813 DT denotes the
T6824 13814-13821 JJ denotes partial
T6825 13822-13827 NN denotes T-bet
T6826 13828-13838 NN denotes dependence
T6827 13839-13841 IN denotes of
T6828 13842-13846 NN denotes GzmB
T6829 13847-13851 NN denotes mRNA
T6830 13852-13862 NN denotes expression
T6831 13863-13866 MD denotes can
T6832 13866-13869 RB denotes not
T6833 13870-13872 VB denotes be
T6834 13873-13884 VB denotes compensated
T6835 13885-13888 IN denotes for
T6836 13889-13891 IN denotes by
T6837 13892-13899 NN denotes Eo-VP16
T8977 13902-13907 NN denotes Runx3
T8978 13908-13916 VB denotes controls
T8979 13917-13925 JJ denotes multiple
T8980 13926-13933 NN denotes aspects
T8981 13934-13936 IN denotes of
T8982 13937-13940 DT denotes the
T8983 13941-13944 NN denotes CTL
T8984 13945-13960 NN denotes differentiation
T8985 13961-13968 NN denotes program
T8986 13968-13969 -COMMA- denotes ,
T8987 13970-13972 IN denotes in
T8988 13973-13977 NN denotes part
T8989 13978-13985 IN denotes through
T8990 13986-13995 NN denotes induction
T8991 13996-13998 IN denotes of
T8992 13999-14004 NN denotes Eomes
T8993 14005-14012 IN denotes Because
T8994 14013-14018 NN denotes Runx3
T8995 14019-14021 VB denotes is
T8996 14022-14028 RB denotes highly
T8997 14029-14038 VB denotes expressed
T8998 14039-14041 IN denotes in
T8999 14042-14052 JJ denotes peripheral
T9000 14053-14057 JJ denotes CD8+
T9001 14058-14059 NN denotes T
T9002 14060-14065 NN denotes cells
T9003 14065-14066 -COMMA- denotes ,
T9004 14067-14070 CC denotes and
T9005 14071-14078 IN denotes because
T9006 14079-14081 IN denotes of
T9007 14082-14085 DT denotes the
T9008 14086-14097 NN denotes T-bet-Runx3
T9009 14098-14109 NN denotes cooperation
T9010 14110-14112 PRP denotes we
T9011 14113-14121 VB denotes observed
T9012 14122-14129 RB denotes earlier
T9013 14130-14132 IN denotes in
T9014 14133-14137 JJ denotes CD4+
T9015 14138-14139 NN denotes T
T9016 14140-14145 NN denotes cells
T9017 14146-14147 -LRB- denotes (
T9018 14147-14149 CD denotes 15
T9019 14149-14150 -RRB- denotes )
T9020 14150-14151 -COMMA- denotes ,
T9021 14152-14154 PRP denotes we
T9022 14155-14163 VB denotes examined
T9023 14164-14167 DT denotes the
T9024 14168-14172 NN denotes role
T9025 14173-14175 IN denotes of
T9026 14176-14181 NN denotes Runx3
T9027 14182-14184 IN denotes in
T9028 14185-14193 NN denotes effector
T9029 14194-14197 NN denotes CTL
T9030 14198-14213 NN denotes differentiation
T9031 14215-14217 PRP denotes We
T9032 14218-14226 VB denotes isolated
T9033 14227-14231 JJ denotes CD8+
T9034 14232-14233 NN denotes T
T9035 14234-14239 NN denotes cells
T9036 14240-14244 IN denotes from
T9037 14245-14253 NN denotes Runx3−/−
T9038 14254-14255 -LRB- denotes (
T9039 14255-14257 NN denotes KO
T9040 14257-14258 -RRB- denotes )
T9041 14259-14263 NN denotes mice
T9042 14264-14266 IN denotes of
T9043 14267-14270 DT denotes the
T9044 14271-14278 JJ denotes outbred
T9045 14279-14282 NN denotes ICR
T9046 14283-14293 NN denotes background
T9047 14294-14297 CC denotes and
T9048 14298-14303 PRP-DOLLAR- denotes their
T9049 14304-14306 JJ denotes WT
T9050 14307-14315 NN denotes Runx3+/+
T9051 14316-14327 NN denotes littermates
T9052 14328-14330 IN denotes by
T9053 14331-14339 JJ denotes positive
T9054 14340-14349 NN denotes selection
T9055 14350-14354 IN denotes with
T9056 14355-14363 JJ denotes anti-CD8
T9057 14364-14372 JJ denotes magnetic
T9058 14373-14378 NN denotes beads
T9059 14379-14380 -LRB- denotes (
T9060 14380-14385 NN denotes Figs.
T9061 14386-14388 NN denotes S1
T9062 14389-14392 CC denotes and
T9063 14393-14395 NN denotes S2
T9064 14395-14396 -COMMA- denotes ,
T9065 14397-14406 JJ denotes available
T9066 14407-14409 IN denotes at
T9067 14410-14414 NN denotes http
T9068 14414-14415 -COLON- denotes :
T9069 14415-14462 NN denotes //www.jem.org/cgi/content/full/jem.20081242/DC1
T9070 14462-14463 -RRB- denotes )
T9071 14465-14475 RB denotes Strikingly
T9072 14475-14476 -COMMA- denotes ,
T9073 14477-14485 NN denotes Runx3−/−
T9074 14486-14490 JJ denotes CD8+
T9075 14491-14492 NN denotes T
T9076 14493-14498 NN denotes cells
T9077 14499-14503 VB denotes were
T9078 14504-14512 RB denotes strongly
T9079 14513-14521 JJ denotes impaired
T9080 14522-14524 IN denotes in
T9081 14525-14530 PRP-DOLLAR- denotes their
T9082 14531-14538 NN denotes ability
T9083 14539-14541 TO denotes to
T9084 14542-14555 VB denotes differentiate
T9085 14556-14560 IN denotes into
T9086 14561-14569 NN denotes effector
T9087 14570-14574 NN denotes CTLs
T9088 14574-14575 -COMMA- denotes ,
T9089 14576-14578 IN denotes as
T9090 14579-14585 VB denotes judged
T9091 14586-14588 IN denotes by
T9092 14589-14599 NN denotes expression
T9093 14600-14602 IN denotes of
T9094 14603-14611 NN denotes perforin
T9095 14611-14612 -COMMA- denotes ,
T9096 14613-14621 NN denotes granzyme
T9097 14622-14623 NN denotes B
T9098 14623-14624 -COMMA- denotes ,
T9099 14625-14628 CC denotes and
T9100 14629-14634 NN denotes IFN-γ
T9101 14635-14636 -LRB- denotes (
T9102 14636-14640 NN denotes Fig.
T9103 14641-14642 CD denotes 3
T9104 14642-14643 -RRB- denotes )
T9105 14645-14653 VB denotes Compared
T9106 14654-14658 IN denotes with
T9107 14659-14661 JJ denotes WT
T9108 14662-14663 NN denotes T
T9109 14664-14669 NN denotes cells
T9110 14669-14670 -COMMA- denotes ,
T9111 14671-14679 NN denotes perforin
T9112 14680-14684 NN denotes mRNA
T9113 14685-14688 CC denotes and
T9114 14689-14696 NN denotes protein
T9115 14697-14707 NN denotes expression
T9116 14708-14712 VB denotes were
T9117 14713-14724 RB denotes essentially
T9118 14725-14737 JJ denotes undetectable
T9119 14738-14740 IN denotes in
T9120 14741-14749 NN denotes Runx3−/−
T9121 14750-14751 NN denotes T
T9122 14752-14757 NN denotes cells
T9123 14758-14760 IN denotes at
T9124 14761-14764 NN denotes day
T9125 14765-14766 CD denotes 6
T9126 14767-14769 IN denotes of
T9127 14770-14777 NN denotes culture
T9128 14778-14779 -LRB- denotes (
T9129 14779-14783 NNP denotes Fig.
T9130 14784-14785 CD denotes 3
T9131 14785-14786 -COMMA- denotes ,
T9132 14787-14788 NN denotes A
T9133 14789-14792 CC denotes and
T9134 14793-14794 NN denotes B
T9135 14794-14795 -RRB- denotes )
T9136 14797-14805 NN denotes Runx3−/−
T9137 14806-14807 NN denotes T
T9138 14808-14813 NN denotes cells
T9139 14814-14818 RB denotes also
T9140 14819-14822 VB denotes had
T9141 14823-14825 DT denotes no
T9142 14826-14836 JJ denotes detectable
T9143 14837-14842 NN denotes Eomes
T9144 14843-14853 NN denotes expression
T9145 14853-14854 -COLON- denotes ;
T9146 14855-14857 IN denotes in
T9147 14858-14866 NN denotes contrast
T9148 14866-14867 -COMMA- denotes ,
T9149 14868-14873 NN denotes T-bet
T9150 14874-14884 NN denotes expression
T9151 14885-14888 VB denotes was
T9152 14889-14899 JJ denotes unimpaired
T9153 14900-14901 -LRB- denotes (
T9154 14901-14905 NN denotes Fig.
T9155 14906-14907 CD denotes 3
T9156 14908-14909 NN denotes A
T9157 14909-14910 -RRB- denotes )
T9158 14912-14923 RB denotes Furthermore
T9159 14923-14924 -COMMA- denotes ,
T9160 14925-14930 NN denotes Runx3
T9161 14931-14934 VB denotes was
T9162 14935-14943 VB denotes required
T9163 14944-14947 IN denotes for
T9164 14948-14955 JJ denotes maximal
T9165 14956-14966 NN denotes production
T9166 14967-14969 IN denotes of
T9167 14970-14975 NN denotes IFN-γ
T9168 14975-14976 -COMMA- denotes ,
T9169 14977-14980 CC denotes but
T9170 14981-14984 RB denotes not
T9171 14985-14988 NN denotes TNF
T9172 14989-14991 CC denotes or
T9173 14992-14996 NN denotes IL-2
T9174 14996-14997 -COMMA- denotes ,
T9175 14998-15000 IN denotes by
T9176 15001-15005 JJ denotes CD8+
T9177 15006-15007 NN denotes T
T9178 15008-15013 NN denotes cells
T9179 15014-15026 VB denotes restimulated
T9180 15027-15029 IN denotes at
T9181 15030-15033 NN denotes day
T9182 15034-15035 CD denotes 6
T9183 15036-15037 -LRB- denotes (
T9184 15037-15041 NN denotes Fig.
T9185 15042-15043 CD denotes 3
T9186 15044-15045 NN denotes C
T9187 15045-15046 -RRB- denotes )
T9188 16186-16188 PRP denotes We
T9189 16189-16199 RB denotes previously
T9190 16200-16208 VB denotes reported
T9191 16209-16213 IN denotes that
T9192 16214-16217 NN denotes Th1
T9193 16218-16222 NN denotes cell
T9194 16223-16238 NN denotes differentiation
T9195 16239-16242 VB denotes was
T9196 16243-16252 VB denotes regulated
T9197 16253-16260 IN denotes through
T9198 16261-16262 DT denotes a
T9199 16263-16275 JJ denotes feed-forward
T9200 16276-16280 NN denotes loop
T9201 16281-16283 IN denotes in
T9202 16284-16289 WDT denotes which
T9203 16290-16295 NN denotes T-bet
T9204 16296-16298 VB denotes is
T9205 16299-16311 VB denotes up-regulated
T9206 16312-16317 RB denotes early
T9207 16318-16321 CC denotes and
T9208 16322-16329 VB denotes induces
T9209 16330-16335 NN denotes Runx3
T9210 16335-16336 -COMMA- denotes ,
T9211 16337-16342 IN denotes after
T9212 16343-16348 WDT denotes which
T9213 16349-16354 NN denotes T-bet
T9214 16355-16358 CC denotes and
T9215 16359-16364 NN denotes Runx3
T9216 16365-16374 VB denotes cooperate
T9217 16375-16377 TO denotes to
T9218 16378-16384 VB denotes induce
T9219 16385-16390 NN denotes IFN-γ
T9220 16391-16394 CC denotes and
T9221 16395-16402 NN denotes silence
T9222 16403-16407 NN denotes IL-4
T9223 16407-16408 -COMMA- denotes ,
T9224 16409-16413 RB denotes thus
T9225 16414-16423 VB denotes promoting
T9226 16424-16430 JJ denotes stable
T9227 16431-16446 NN denotes differentiation
T9228 16447-16453 IN denotes toward
T9229 16454-16457 DT denotes the
T9230 16458-16461 NN denotes Th1
T9231 16462-16469 NN denotes lineage
T9232 16470-16471 -LRB- denotes (
T9233 16471-16473 CD denotes 15
T9234 16473-16474 -COMMA- denotes ,
T9235 16475-16477 CD denotes 22
T9236 16477-16478 -RRB- denotes )
T9237 16480-16487 IN denotes Because
T9238 16488-16489 -LRB- denotes (
T9239 16489-16490 DT denotes a
T9240 16490-16491 -RRB- denotes )
T9241 16492-16497 NN denotes Runx3
T9242 16498-16506 VB denotes appeared
T9243 16507-16516 JJ denotes necessary
T9244 16517-16520 IN denotes for
T9245 16521-16526 NN denotes Eomes
T9246 16527-16536 NN denotes induction
T9247 16537-16538 -LRB- denotes (
T9248 16538-16542 NN denotes Fig.
T9249 16543-16544 CD denotes 3
T9250 16545-16546 NN denotes A
T9251 16546-16547 -RRB- denotes )
T9252 16547-16548 -COMMA- denotes ,
T9253 16549-16550 -LRB- denotes (
T9254 16550-16551 NN denotes b
T9255 16551-16552 -RRB- denotes )
T9256 16553-16556 DT denotes the
T9257 16557-16565 NN denotes kinetics
T9258 16566-16568 IN denotes of
T9259 16569-16574 NN denotes Eomes
T9260 16575-16585 NN denotes expression
T9261 16586-16596 VB denotes paralleled
T9262 16597-16602 DT denotes those
T9263 16603-16605 IN denotes of
T9264 16606-16614 NN denotes perforin
T9265 16615-16625 NN denotes expression
T9266 16626-16627 -LRB- denotes (
T9267 16627-16631 NN denotes Fig.
T9268 16632-16633 CD denotes 2
T9269 16633-16634 -RRB- denotes )
T9270 16634-16635 -COMMA- denotes ,
T9271 16636-16639 CC denotes and
T9272 16640-16641 -LRB- denotes (
T9273 16641-16642 NN denotes c
T9274 16642-16643 -RRB- denotes )
T9275 16644-16658 NN denotes overexpression
T9276 16659-16661 IN denotes of
T9277 16662-16669 NN denotes Eo-VP16
T9278 16670-16672 IN denotes in
T9279 16673-16679 CC denotes either
T9280 16680-16682 NN denotes WT
T9281 16683-16685 CC denotes or
T9282 16686-16701 JJ denotes T-bet–deficient
T9283 16702-16703 NN denotes T
T9284 16704-16709 NN denotes cells
T9285 16710-16713 VB denotes led
T9286 16714-16716 TO denotes to
T9287 16717-16719 DT denotes an
T9288 16720-16728 NN denotes increase
T9289 16729-16731 IN denotes in
T9290 16732-16736 CC denotes both
T9291 16737-16745 NN denotes perforin
T9292 16746-16749 CC denotes and
T9293 16750-16755 NN denotes IFN-γ
T9294 16756-16766 NN denotes expression
T9295 16767-16768 -LRB- denotes (
T9296 16768-16772 NN denotes Fig.
T9297 16773-16774 CD denotes 2
T9298 16774-16775 -COMMA- denotes ,
T9299 16776-16777 NN denotes B
T9300 16778-16781 CC denotes and
T9301 16782-16783 NN denotes D
T9302 16783-16784 -RRB- denotes )
T9303 16784-16785 -COMMA- denotes ,
T9304 16786-16788 PRP denotes we
T9305 16789-16794 VB denotes asked
T9306 16795-16802 IN denotes whether
T9307 16803-16806 NN denotes CTL
T9308 16807-16822 NN denotes differentiation
T9309 16823-16826 VB denotes was
T9310 16827-16831 RB denotes also
T9311 16832-16843 RB denotes potentially
T9312 16844-16853 VB denotes regulated
T9313 16854-16856 IN denotes by
T9314 16857-16858 DT denotes a
T9315 16859-16871 JJ denotes feed-forward
T9316 16872-16876 NN denotes loop
T9317 16877-16886 VB denotes involving
T9318 16887-16892 DT denotes these
T9319 16893-16897 JJ denotes same
T9320 16898-16901 CD denotes two
T9321 16902-16909 NN denotes classes
T9322 16910-16912 IN denotes of
T9323 16913-16917 NN denotes Runx
T9324 16918-16921 CC denotes and
T9325 16922-16927 NN denotes T-box
T9326 16928-16941 NN denotes transcription
T9327 16942-16949 NN denotes factors
T9328 16951-16963 RB denotes Specifically
T9329 16963-16964 -COMMA- denotes ,
T9330 16965-16967 PRP denotes we
T9331 16968-16973 VB denotes asked
T9332 16974-16981 IN denotes whether
T9333 16982-16987 NN denotes Runx3
T9334 16987-16988 -COMMA- denotes ,
T9335 16989-16994 WDT denotes which
T9336 16995-16998 VB denotes was
T9337 16999-17008 JJ denotes necessary
T9338 17009-17012 IN denotes for
T9339 17013-17018 NN denotes Eomes
T9340 17019-17028 NN denotes induction
T9341 17028-17029 -COMMA- denotes ,
T9342 17030-17034 RB denotes then
T9343 17035-17045 VB denotes cooperated
T9344 17046-17050 IN denotes with
T9345 17051-17056 NN denotes Eomes
T9346 17057-17059 TO denotes to
T9347 17060-17068 VB denotes regulate
T9348 17069-17082 NN denotes transcription
T9349 17083-17085 IN denotes of
T9350 17086-17089 DT denotes the
T9351 17090-17098 NN denotes effector
T9352 17099-17102 NN denotes CTL
T9353 17103-17110 NN denotes markers
T9354 17111-17119 NN denotes perforin
T9355 17119-17120 -COMMA- denotes ,
T9356 17121-17126 NN denotes IFN-γ
T9357 17126-17127 -COMMA- denotes ,
T9358 17128-17131 CC denotes and
T9359 17132-17140 NN denotes granzyme
T9360 17141-17142 NN denotes B
T9361 17144-17146 TO denotes To
T9362 17147-17151 VB denotes test
T9363 17152-17156 DT denotes this
T9364 17157-17167 NN denotes hypothesis
T9365 17167-17168 -COMMA- denotes ,
T9366 17169-17171 PRP denotes we
T9367 17172-17176 VB denotes used
T9368 17177-17186 NN denotes chromatin
T9369 17187-17206 NN denotes immunoprecipitation
T9370 17207-17208 -LRB- denotes (
T9371 17208-17212 NN denotes ChIP
T9372 17212-17213 -RRB- denotes )
T9373 17214-17220 NN denotes assays
T9374 17221-17223 TO denotes to
T9375 17224-17227 VB denotes ask
T9376 17228-17235 IN denotes whether
T9377 17236-17241 NN denotes Eomes
T9378 17242-17245 CC denotes and
T9379 17246-17251 NN denotes Runx3
T9380 17252-17257 VB denotes bound
T9381 17258-17268 JJ denotes regulatory
T9382 17269-17276 NN denotes regions
T9383 17277-17279 IN denotes of
T9384 17280-17283 DT denotes the
T9385 17284-17288 NN denotes Prf1
T9386 17288-17289 -COMMA- denotes ,
T9387 17290-17294 NN denotes Ifng
T9388 17294-17295 -COMMA- denotes ,
T9389 17296-17299 CC denotes and
T9390 17300-17304 NN denotes Gzmb
T9391 17305-17310 NN denotes genes
T9392 17311-17312 -LRB- denotes (
T9393 17312-17316 NN denotes Fig.
T9394 17317-17318 CD denotes 3
T9395 17319-17320 NN denotes D
T9396 17320-17321 -RRB- denotes )
T9397 17323-17327 DT denotes Both
T9398 17328-17336 NN denotes proteins
T9399 17337-17347 VB denotes associated
T9400 17348-17352 IN denotes with
T9401 17353-17357 NN denotes gene
T9402 17358-17368 JJ denotes regulatory
T9403 17369-17376 NN denotes regions
T9404 17377-17379 IN denotes in
T9405 17380-17394 VB denotes differentiated
T9406 17395-17399 NN denotes CTLs
T9407 17401-17406 NN denotes Runx3
T9408 17407-17412 VB denotes bound
T9409 17413-17415 TO denotes to
T9410 17416-17419 DT denotes the
T9411 17420-17424 NN denotes Prf1
T9412 17425-17428 CC denotes and
T9413 17429-17433 NN denotes Gzmb
T9414 17434-17447 NN denotes transcription
T9415 17448-17453 NN denotes start
T9416 17454-17459 NN denotes sites
T9417 17460-17461 -LRB- denotes (
T9418 17461-17464 NN denotes TSS
T9419 17464-17465 -RRB- denotes )
T9420 17465-17466 -COLON- denotes ;
T9421 17467-17469 TO denotes to
T9422 17470-17471 DT denotes a
T9423 17472-17477 VB denotes known
T9424 17478-17482 NN denotes IL-2
T9425 17483-17493 JJ denotes responsive
T9426 17494-17502 NN denotes enhancer
T9427 17503-17510 JJ denotes located
T9428 17511-17515 IN denotes near
T9429 17516-17518 NN denotes −1
T9430 17519-17521 NN denotes kb
T9431 17522-17524 IN denotes of
T9432 17525-17528 DT denotes the
T9433 17529-17533 NN denotes Prf1
T9434 17534-17538 NN denotes gene
T9435 17539-17540 -LRB- denotes (
T9436 17540-17542 CD denotes 23
T9437 17542-17543 -RRB- denotes )
T9438 17543-17544 -COLON- denotes ;
T9439 17545-17547 TO denotes to
T9440 17548-17551 DT denotes the
T9441 17552-17558 JJ denotes distal
T9442 17559-17571 JJ denotes CTL-specific
T9443 17572-17577 NN denotes DNase
T9444 17578-17579 CD denotes I
T9445 17580-17594 JJ denotes hypersensitive
T9446 17595-17599 NN denotes site
T9447 17600-17601 CD denotes 9
T9448 17602-17604 IN denotes in
T9449 17605-17608 DT denotes the
T9450 17609-17613 NN denotes Prf1
T9451 17614-17619 NN denotes locus
T9452 17620-17621 -LRB- denotes (
T9453 17621-17623 CD denotes 24
T9454 17623-17624 -RRB- denotes )
T9455 17624-17625 -COLON- denotes ;
T9456 17626-17628 TO denotes to
T9457 17629-17632 DT denotes the
T9458 17633-17637 NN denotes Ifng
T9459 17638-17646 NN denotes promoter
T9460 17647-17651 IN denotes near
T9461 17652-17655 DT denotes the
T9462 17656-17659 NN denotes TSS
T9463 17659-17660 -COMMA- denotes ,
T9464 17661-17663 IN denotes as
T9465 17664-17674 RB denotes previously
T9466 17675-17683 VB denotes reported
T9467 17684-17687 IN denotes for
T9468 17688-17691 NN denotes Th1
T9469 17692-17697 NN denotes cells
T9470 17698-17699 -LRB- denotes (
T9471 17699-17701 CD denotes 10
T9472 17701-17702 -RRB- denotes )
T9473 17702-17703 -COLON- denotes ;
T9474 17704-17707 CC denotes and
T9475 17708-17710 TO denotes to
T9476 17711-17718 JJ denotes several
T9477 17719-17724 NN denotes DNase
T9478 17725-17726 CD denotes I
T9479 17727-17741 JJ denotes hypersensitive
T9480 17742-17747 NN denotes sites
T9481 17748-17750 IN denotes in
T9482 17751-17754 DT denotes the
T9483 17755-17759 NN denotes Ifng
T9484 17760-17765 NN denotes locus
T9485 17766-17767 -LRB- denotes (
T9486 17767-17771 NN denotes Fig.
T9487 17772-17773 CD denotes 3
T9488 17774-17775 NN denotes D
T9489 17776-17779 CC denotes and
T9490 17780-17783 RB denotes not
T9491 17784-17792 VB denotes depicted
T9492 17792-17793 -RRB- denotes )
T9493 17794-17795 -LRB- denotes (
T9494 17795-17797 CD denotes 25
T9495 17797-17798 -RRB- denotes )
T9496 17800-17805 NN denotes Eomes
T9497 17806-17811 VB denotes bound
T9498 17812-17821 RB denotes primarily
T9499 17822-17824 TO denotes to
T9500 17825-17828 DT denotes the
T9501 17829-17833 NN denotes Prf1
T9502 17834-17837 NN denotes TSS
T9503 17838-17841 CC denotes and
T9504 17842-17845 DT denotes the
T9505 17846-17848 NN denotes −1
T9506 17849-17851 NN denotes kb
T9507 17852-17860 NN denotes enhancer
T9508 17860-17861 -COLON- denotes ;
T9509 17862-17866 DT denotes this
T9510 17867-17874 NN denotes binding
T9511 17875-17878 VB denotes was
T9512 17879-17892 RB denotes substantially
T9513 17893-17900 JJ denotes greater
T9514 17901-17905 IN denotes than
T9515 17906-17910 DT denotes that
T9516 17911-17919 VB denotes observed
T9517 17920-17922 IN denotes at
T9518 17923-17926 DT denotes the
T9519 17927-17935 NN denotes promoter
T9520 17936-17938 IN denotes of
T9521 17939-17942 DT denotes the
T9522 17943-17948 NN denotes Il2rb
T9523 17949-17953 NN denotes gene
T9524 17953-17954 -COMMA- denotes ,
T9525 17955-17956 DT denotes a
T9526 17957-17962 JJ denotes known
T9527 17963-17969 JJ denotes direct
T9528 17970-17976 NN denotes target
T9529 17977-17979 IN denotes of
T9530 17980-17985 NN denotes Eomes
T9531 17986-17987 -LRB- denotes (
T9532 17987-17988 CD denotes 8
T9533 17988-17989 -RRB- denotes )
T9534 17989-17990 -COMMA- denotes ,
T9535 17991-17994 CC denotes and
T9536 17995-18005 JJ denotes comparable
T9537 18006-18008 TO denotes to
T9538 18009-18013 DT denotes that
T9539 18014-18022 VB denotes observed
T9540 18023-18025 IN denotes at
T9541 18026-18029 DT denotes the
T9542 18030-18034 NNP denotes Ifng
T9543 18035-18038 NN denotes TSS
T9544 18038-18039 -COMMA- denotes ,
T9545 18040-18041 DT denotes a
T9546 18042-18047 VB denotes known
T9547 18048-18054 NN denotes target
T9548 18055-18057 IN denotes of
T9549 18058-18063 NN denotes T-box
T9550 18064-18072 NN denotes proteins
T9551 18073-18075 IN denotes in
T9552 18076-18080 CC denotes both
T9553 18081-18084 NN denotes Th1
T9554 18085-18088 CC denotes and
T9555 18089-18093 JJ denotes CD8+
T9556 18094-18095 NN denotes T
T9557 18096-18101 NN denotes cells
T9558 18102-18103 -LRB- denotes (
T9559 18103-18107 NN denotes Fig.
T9560 18108-18109 CD denotes 3
T9561 18110-18111 NN denotes D
T9562 18111-18112 -RRB- denotes )
T9563 18113-18114 -LRB- denotes (
T9564 18114-18116 CD denotes 17
T9565 18116-18117 -RRB- denotes )
T9566 18119-18121 TO denotes To
T9567 18122-18131 VB denotes determine
T9568 18132-18139 IN denotes whether
T9569 18140-18145 NN denotes Runx3
T9570 18146-18156 VB denotes controlled
T9571 18157-18160 DT denotes the
T9572 18161-18171 NN denotes expression
T9573 18172-18174 IN denotes of
T9574 18175-18178 NN denotes CTL
T9575 18179-18187 NN denotes effector
T9576 18188-18193 NN denotes genes
T9577 18194-18201 IN denotes through
T9578 18202-18205 PRP-DOLLAR- denotes its
T9579 18206-18215 NN denotes induction
T9580 18216-18218 IN denotes of
T9581 18219-18224 NN denotes Eomes
T9582 18224-18225 -COMMA- denotes ,
T9583 18226-18228 PRP denotes we
T9584 18229-18241 RB denotes retrovirally
T9585 18242-18251 VB denotes expressed
T9586 18252-18257 NN denotes Runx3
T9587 18258-18261 CC denotes and
T9588 18262-18269 NN denotes Eo-VP16
T9589 18270-18272 IN denotes in
T9590 18273-18277 JJ denotes CD8+
T9591 18278-18279 NN denotes T
T9592 18280-18285 NN denotes cells
T9593 18286-18290 IN denotes from
T9594 18291-18299 NN denotes Runx3−/−
T9595 18300-18304 NN denotes mice
T9596 18306-18313 IN denotes Because
T9597 18314-18316 IN denotes of
T9598 18317-18320 DT denotes the
T9599 18321-18328 JJ denotes limited
T9600 18329-18335 NN denotes number
T9601 18336-18338 IN denotes of
T9602 18339-18343 JJ denotes CD8+
T9603 18344-18345 NN denotes T
T9604 18346-18351 NN denotes cells
T9605 18352-18354 IN denotes in
T9606 18355-18360 DT denotes these
T9607 18361-18365 NN denotes mice
T9608 18365-18366 -COMMA- denotes ,
T9609 18367-18370 CC denotes and
T9610 18371-18378 IN denotes because
T9611 18379-18381 PRP denotes we
T9612 18382-18385 VB denotes saw
T9613 18386-18388 DT denotes no
T9614 18389-18399 NN denotes difference
T9615 18400-18407 IN denotes between
T9616 18408-18416 NN denotes Runx3−/−
T9617 18417-18425 NN denotes CD8+CD4−
T9618 18426-18428 NN denotes SP
T9619 18429-18432 CC denotes and
T9620 18433-18441 JJ denotes CD8+CD4+
T9621 18442-18444 NN denotes DP
T9622 18445-18450 NN denotes cells
T9623 18451-18453 IN denotes in
T9624 18454-18457 PRP-DOLLAR- denotes our
T9625 18458-18466 JJ denotes previous
T9626 18467-18478 NN denotes experiments
T9627 18478-18479 -COMMA- denotes ,
T9628 18480-18482 PRP denotes we
T9629 18483-18487 VB denotes used
T9630 18488-18493 JJ denotes total
T9631 18494-18502 NN denotes Runx3−/−
T9632 18503-18507 JJ denotes CD8+
T9633 18508-18509 NN denotes T
T9634 18510-18515 NN denotes cells
T9635 18516-18523 IN denotes without
T9636 18524-18531 JJ denotes further
T9637 18532-18545 NN denotes fractionation
T9638 18546-18548 IN denotes as
T9639 18549-18559 NN denotes recipients
T9640 18560-18563 IN denotes for
T9641 18564-18574 JJ denotes retroviral
T9642 18575-18587 NN denotes transduction
T9643 18589-18603 NN denotes Reconstitution
T9644 18604-18606 IN denotes of
T9645 18607-18615 NN denotes Runx3−/−
T9646 18616-18620 JJ denotes CD8+
T9647 18621-18622 NN denotes T
T9648 18623-18628 NN denotes cells
T9649 18629-18633 IN denotes with
T9650 18634-18639 NN denotes Runx3
T9651 18640-18648 VB denotes restored
T9652 18649-18659 NN denotes expression
T9653 18660-18662 IN denotes of
T9654 18663-18668 NN denotes Eomes
T9655 18669-18671 RB denotes as
T9656 18672-18676 RB denotes well
T9657 18677-18679 IN denotes as
T9658 18680-18688 NN denotes perforin
T9659 18688-18689 -COMMA- denotes ,
T9660 18690-18698 NN denotes granzyme
T9661 18699-18700 NN denotes B
T9662 18700-18701 -COMMA- denotes ,
T9663 18702-18705 CC denotes and
T9664 18706-18711 NN denotes IFN-γ
T9665 18712-18713 -LRB- denotes (
T9666 18713-18717 NN denotes Fig.
T9667 18718-18719 CD denotes 4
T9668 18719-18720 -COMMA- denotes ,
T9669 18721-18722 NN denotes A
T9670 18723-18726 CC denotes and
T9671 18727-18728 NN denotes B
T9672 18728-18729 -RRB- denotes )
T9673 18731-18733 IN denotes In
T9674 18734-18742 NN denotes addition
T9675 18742-18743 -COMMA- denotes ,
T9676 18744-18752 NN denotes Runx3−/−
T9677 18753-18754 NN denotes T
T9678 18755-18760 NN denotes cells
T9679 18761-18767 VB denotes showed
T9680 18768-18769 DT denotes a
T9681 18770-18782 JJ denotes compensatory
T9682 18783-18796 NN denotes up-regulation
T9683 18797-18799 IN denotes of
T9684 18800-18805 NN denotes Runx1
T9685 18805-18806 -COMMA- denotes ,
T9686 18807-18812 WDT denotes which
T9687 18813-18816 VB denotes was
T9688 18817-18827 VB denotes suppressed
T9689 18828-18832 IN denotes upon
T9690 18833-18847 NN denotes reconstitution
T9691 18848-18852 IN denotes with
T9692 18853-18858 NN denotes Runx3
T9693 18858-18859 -COMMA- denotes ,
T9694 18860-18870 VB denotes indicating
T9695 18871-18875 IN denotes that
T9696 18876-18881 NN denotes Runx1
T9697 18882-18884 VB denotes is
T9698 18885-18886 DT denotes a
T9699 18887-18893 NN denotes target
T9700 18894-18896 IN denotes of
T9701 18897-18907 NN denotes repression
T9702 18908-18910 IN denotes by
T9703 18911-18916 NN denotes Runx3
T9704 18918-18925 RB denotes Notably
T9705 18925-18926 -COMMA- denotes ,
T9706 18927-18934 NN denotes Eo-VP16
T9707 18935-18938 VB denotes did
T9708 18939-18942 RB denotes not
T9709 18943-18954 VB denotes up-regulate
T9710 18955-18963 NN denotes perforin
T9711 18964-18974 NN denotes expression
T9712 18975-18979 WRB denotes when
T9713 18980-18989 VB denotes expressed
T9714 18990-18992 IN denotes in
T9715 18993-19001 NN denotes Runx3−/−
T9716 19002-19007 NN denotes cells
T9717 19007-19008 -COMMA- denotes ,
T9718 19009-19013 RB denotes even
T9719 19014-19020 IN denotes though
T9720 19021-19023 PRP denotes it
T9721 19024-19032 VB denotes restored
T9722 19033-19036 DT denotes the
T9723 19037-19045 NN denotes capacity
T9724 19046-19048 TO denotes to
T9725 19049-19055 VB denotes induce
T9726 19056-19061 NN denotes IFN-γ
T9727 19062-19072 NN denotes expression
T9728 19073-19077 IN denotes upon
T9729 19078-19081 NN denotes TCR
T9730 19082-19095 NN denotes restimulation
T9731 19096-19097 -LRB- denotes (
T9732 19097-19101 NN denotes Fig.
T9733 19102-19103 CD denotes 4
T9734 19103-19104 -COMMA- denotes ,
T9735 19105-19106 NN denotes A
T9736 19107-19110 CC denotes and
T9737 19111-19112 NN denotes B
T9738 19112-19113 -RRB- denotes )
T9739 19115-19119 DT denotes This
T9740 19120-19126 NN denotes result
T9741 19127-19135 VB denotes suggests
T9742 19136-19144 RB denotes strongly
T9743 19145-19149 IN denotes that
T9744 19150-19158 NN denotes perforin
T9745 19159-19169 NN denotes expression
T9746 19170-19178 VB denotes requires
T9747 19179-19184 NN denotes Runx3
T9748 19185-19188 CC denotes and
T9749 19189-19194 NN denotes Eomes
T9750 20867-20869 IN denotes As
T9751 20870-20878 VB denotes expected
T9752 20879-20883 IN denotes from
T9753 20884-20889 PRP-DOLLAR- denotes their
T9754 20890-20896 NN denotes defect
T9755 20897-20899 IN denotes in
T9756 20900-20908 NN denotes perforin
T9757 20909-20912 CC denotes and
T9758 20913-20921 NN denotes granzyme
T9759 20922-20923 NN denotes B
T9760 20924-20934 NN denotes expression
T9761 20934-20935 -COMMA- denotes ,
T9762 20936-20944 NN denotes Runx3−/−
T9763 20945-20949 JJ denotes CD8+
T9764 20950-20951 NN denotes T
T9765 20952-20957 NN denotes cells
T9766 20958-20964 VB denotes showed
T9767 20965-20974 JJ denotes defective
T9768 20975-20984 JJ denotes cytolytic
T9769 20985-20993 NN denotes activity
T9770 20994-20996 IN denotes in
T9771 20997-20998 DT denotes a
T9772 20999-21004 JJ denotes mixed
T9773 21005-21015 NN denotes lymphocyte
T9774 21016-21024 NN denotes reaction
T9775 21025-21026 -LRB- denotes (
T9776 21026-21028 CD denotes 12
T9777 21028-21029 -RRB- denotes )
T9778 21031-21038 RB denotes However
T9779 21038-21039 -COMMA- denotes ,
T9780 21040-21054 JJ denotes TCR-stimulated
T9781 21055-21063 NN denotes Runx3−/−
T9782 21064-21068 JJ denotes CD8+
T9783 21069-21074 NN denotes cells
T9784 21075-21079 VB denotes were
T9785 21080-21082 RB denotes as
T9786 21083-21092 JJ denotes effective
T9787 21093-21095 IN denotes as
T9788 21096-21098 JJ denotes WT
T9789 21099-21104 NN denotes cells
T9790 21105-21107 IN denotes in
T9791 21108-21115 VB denotes killing
T9792 21116-21121 NN denotes tumor
T9793 21122-21127 NN denotes cells
T9794 21128-21130 IN denotes in
T9795 21131-21132 DT denotes a
T9796 21133-21143 JJ denotes redirected
T9797 21144-21147 NN denotes CTL
T9798 21148-21153 NN denotes assay
T9799 21154-21155 -LRB- denotes (
T9800 21155-21157 CD denotes 12
T9801 21157-21158 -RRB- denotes )
T9802 21160-21171 RB denotes Furthermore
T9803 21171-21172 -COMMA- denotes ,
T9804 21173-21177 JJ denotes CD8+
T9805 21178-21183 NN denotes cells
T9806 21184-21188 IN denotes from
T9807 21189-21192 DT denotes the
T9808 21193-21203 JJ denotes peritoneal
T9809 21204-21210 NN denotes cavity
T9810 21211-21213 IN denotes of
T9811 21214-21222 NN denotes Runx3−/−
T9812 21223-21227 NN denotes mice
T9813 21228-21237 VB denotes immunized
T9814 21238-21242 IN denotes with
T9815 21243-21250 JJ denotes certain
T9816 21251-21256 NN denotes tumor
T9817 21257-21262 NN denotes cells
T9818 21263-21274 RB denotes effectively
T9819 21275-21281 VB denotes killed
T9820 21282-21287 DT denotes these
T9821 21288-21295 NN denotes targets
T9822 21296-21297 -LRB- denotes (
T9823 21297-21299 CD denotes 13
T9824 21299-21300 -RRB- denotes )
T9825 21302-21311 RB denotes Therefore
T9826 21311-21312 -COMMA- denotes ,
T9827 21313-21321 IN denotes although
T9828 21322-21332 NN denotes activation
T9829 21333-21335 IN denotes of
T9830 21336-21339 DT denotes the
T9831 21340-21357 NN denotes perforin/granzyme
T9832 21358-21359 NN denotes B
T9833 21360-21369 NN denotes machinery
T9834 21370-21372 VB denotes is
T9835 21373-21382 JJ denotes defective
T9836 21383-21385 IN denotes in
T9837 21386-21394 NN denotes Runx3−/−
T9838 21395-21399 JJ denotes CD8+
T9839 21400-21405 NN denotes cells
T9840 21405-21406 -COMMA- denotes ,
T9841 21407-21412 DT denotes these
T9842 21413-21418 NN denotes cells
T9843 21419-21422 VB denotes are
T9844 21423-21426 RB denotes not
T9845 21427-21435 RB denotes entirely
T9846 21436-21442 JJ denotes devoid
T9847 21443-21445 IN denotes of
T9848 21446-21455 JJ denotes cytolytic
T9849 21456-21464 NN denotes activity
T9850 21465-21468 CC denotes and
T9851 21469-21474 MD denotes could
T9852 21475-21480 RB denotes still
T9853 21481-21492 RB denotes effectively
T9854 21493-21497 VB denotes kill
T9855 21498-21505 NN denotes targets
T9856 21505-21506 -COMMA- denotes ,
T9857 21507-21515 RB denotes possibly
T9858 21516-21518 IN denotes by
T9859 21519-21530 JJ denotes alternative
T9860 21531-21541 NN denotes mechanisms
T9861 21542-21546 JJ denotes such
T9862 21547-21549 IN denotes as
T9863 21550-21553 DT denotes the
T9864 21554-21561 NN denotes Fas–Fas
T9865 21562-21568 NN denotes ligand
T9866 21569-21576 NN denotes pathway
T14019 25300-25310 NN denotes Antibodies
T14020 25311-25314 CC denotes and
T14021 25315-25323 NN denotes reagents
T14022 25325-25328 DT denotes The
T14023 25329-25338 JJ denotes following
T14024 25339-25349 NN denotes antibodies
T14025 25350-25354 VB denotes used
T14026 25355-25358 IN denotes for
T14027 25359-25372 JJ denotes intracellular
T14028 25373-25375 CC denotes or
T14029 25376-25383 NN denotes surface
T14030 25384-25390 NN denotes stains
T14031 25391-25395 VB denotes were
T14032 25396-25404 VB denotes obtained
T14033 25405-25409 IN denotes from
T14034 25410-25421 NN denotes eBioscience
T14035 25421-25422 -COLON- denotes :
T14036 25423-25432 NN denotes anti–IL-2
T14037 25432-25433 -COMMA- denotes ,
T14038 25434-25444 JJ denotes anti–IFN-γ
T14039 25444-25445 -COMMA- denotes ,
T14040 25446-25454 JJ denotes anti-TNF
T14041 25454-25455 -COMMA- denotes ,
T14042 25456-25469 NN denotes anti–granzyme
T14043 25470-25471 NN denotes B
T14044 25471-25472 -COMMA- denotes ,
T14045 25473-25481 NN denotes anti-CD8
T14046 25481-25482 -COMMA- denotes ,
T14047 25483-25492 NN denotes anti-CD25
T14048 25492-25493 -COMMA- denotes ,
T14049 25494-25497 CC denotes and
T14050 25498-25507 NN denotes anti-CD44
T14051 25509-25518 NN denotes Anti-CD69
T14052 25519-25522 VB denotes was
T14053 25523-25532 VB denotes purchased
T14054 25533-25537 IN denotes from
T14055 25538-25540 NN denotes BD
T14056 25542-25545 IN denotes For
T14057 25546-25550 NN denotes ChIP
T14058 25551-25562 NN denotes experiments
T14059 25562-25563 -COMMA- denotes ,
T14060 25564-25567 DT denotes the
T14061 25568-25578 JJ denotes anti-Eomes
T14062 25579-25587 NN denotes antibody
T14063 25588-25591 VB denotes was
T14064 25592-25600 VB denotes obtained
T14065 25601-25605 IN denotes from
T14066 25606-25611 NN denotes Abcam
T14067 25612-25615 CC denotes and
T14068 25616-25619 DT denotes the
T14069 25620-25630 JJ denotes anti-Runx3
T14070 25631-25639 NN denotes antibody
T14071 25640-25643 VB denotes was
T14072 25644-25652 VB denotes produced
T14073 25653-25655 IN denotes by
T14074 25656-25659 DT denotes the
T14075 25660-25666 NNP denotes Groner
T14076 25667-25677 NN denotes laboratory
T14077 25679-25682 DT denotes The
T14078 25683-25692 VB denotes following
T14079 25693-25703 NN denotes antibodies
T14080 25704-25708 VB denotes were
T14081 25709-25713 VB denotes used
T14082 25714-25717 IN denotes for
T14083 25718-25732 NN denotes immunoblotting
T14084 25732-25733 -COLON- denotes :
T14085 25734-25746 NN denotes antiperforin
T14086 25747-25748 -LRB- denotes (
T14087 25748-25753 NN denotes Abcam
T14088 25753-25754 -RRB- denotes )
T14089 25754-25755 -COMMA- denotes ,
T14090 25756-25766 JJ denotes anti-Eomes
T14091 25767-25768 -LRB- denotes (
T14092 25768-25773 NN denotes Abcam
T14093 25773-25774 -RRB- denotes )
T14094 25774-25775 -COMMA- denotes ,
T14095 25776-25779 CC denotes and
T14096 25780-25791 NN denotes anti–Pol-II
T14097 25792-25793 -LRB- denotes (
T14098 25793-25798 NNP denotes Santa
T14099 25799-25803 NNP denotes Cruz
T14100 25804-25817 NNP denotes Biotechnology
T14101 25817-25818 -COMMA- denotes ,
T14102 25819-25823 NNP denotes Inc.
T14103 25823-25824 -RRB- denotes )
T14104 25826-25829 DT denotes The
T14105 25830-25835 JJ denotes T-bet
T14106 25836-25844 NN denotes antibody
T14107 25845-25848 VB denotes was
T14108 25849-25857 VB denotes provided
T14109 25858-25860 IN denotes by
T14110 25861-25863 NNP denotes L.
T14111 25864-25872 NNP denotes Glimcher
T14112 25873-25874 -LRB- denotes (
T14113 25874-25881 NNP denotes Harvard
T14114 25882-25888 NNP denotes School
T14115 25889-25891 IN denotes of
T14116 25892-25898 NNP denotes Public
T14117 25899-25905 NNP denotes Health
T14118 25905-25906 -COMMA- denotes ,
T14119 25907-25913 NNP denotes Boston
T14120 25913-25914 -COMMA- denotes ,
T14121 25915-25917 NNP denotes MA
T14122 25917-25918 -RRB- denotes )
T14123 25920-25923 DT denotes The
T14124 25924-25933 VB denotes following
T14125 25934-25942 NN denotes reagents
T14126 25943-25947 VB denotes were
T14127 25948-25952 VB denotes used
T14128 25953-25956 IN denotes for
T14129 25957-25960 DT denotes the
T14130 25961-25972 NN denotes experiments
T14131 25973-25982 VB denotes presented
T14132 25983-25985 IN denotes in
T14133 25986-25990 DT denotes this
T14134 25991-25997 NN denotes report
T14135 25997-25998 -COLON- denotes :
T14136 25999-26006 NN denotes Annexin
T14137 26007-26013 NNP denotes V–FITC
T14138 26014-26023 NNP denotes Apoptosis
T14139 26024-26033 NN denotes Detection
T14140 26034-26037 NN denotes Kit
T14141 26038-26039 -LRB- denotes (
T14142 26039-26041 NN denotes BD
T14143 26041-26042 -RRB- denotes )
T14144 26042-26043 -COMMA- denotes ,
T14145 26044-26047 NN denotes CD8
T14146 26048-26056 JJ denotes Negative
T14147 26057-26066 NN denotes Isolation
T14148 26067-26070 NN denotes Kit
T14149 26071-26072 -LRB- denotes (
T14150 26072-26082 NN denotes Invitrogen
T14151 26082-26083 -RRB- denotes )
T14152 26083-26084 -COMMA- denotes ,
T14153 26085-26088 NN denotes CD8
T14154 26089-26099 NN denotes MicroBeads
T14155 26100-26101 -LRB- denotes (
T14156 26101-26109 NNP denotes Miltenyi
T14157 26110-26116 NNP denotes Biotec
T14158 26116-26117 -RRB- denotes )
T14159 26117-26118 -COMMA- denotes ,
T14160 26119-26122 CC denotes and
T14161 26123-26127 NNP denotes SYBR
T14162 26128-26133 NNP denotes Green
T14163 26134-26137 NN denotes PCR
T14164 26138-26142 NNP denotes Core
T14165 26143-26151 NNP denotes Reagents
T14166 26152-26153 -LRB- denotes (
T14167 26153-26160 NNP denotes Applied
T14168 26161-26171 NNP denotes Biosystems
T14169 26171-26172 -RRB- denotes )
T14170 26174-26177 DT denotes The
T14171 26178-26182 NN denotes Gp33
T14172 26183-26190 NN denotes peptide
T14173 26191-26192 -LRB- denotes (
T14174 26192-26201 NN denotes KAVYNFATC
T14175 26201-26202 -RRB- denotes )
T14176 26203-26206 VB denotes was
T14177 26207-26218 VB denotes synthesized
T14178 26219-26221 IN denotes by
T14179 26222-26225 DT denotes the
T14180 26226-26231 NNP denotes Tufts
T14181 26232-26242 NNP denotes University
T14182 26243-26247 NNP denotes Core
T14183 26248-26256 NNP denotes Facility
T14184 26256-26257 -COMMA- denotes ,
T14185 26258-26261 CC denotes and
T14186 26262-26264 CD denotes 10
T14187 26265-26267 NN denotes mM
T14188 26268-26270 IN denotes of
T14189 26271-26276 NN denotes stock
T14190 26277-26286 NN denotes solutions
T14191 26287-26290 VB denotes was
T14192 26291-26299 VB denotes prepared
T14193 26300-26302 IN denotes in
T14194 26303-26307 NN denotes DMSO
T14607 26310-26319 NN denotes Isolation
T14608 26320-26323 CC denotes and
T14609 26324-26331 NN denotes culture
T14610 26332-26334 IN denotes of
T14611 26335-26342 JJ denotes primary
T14612 26343-26347 JJ denotes CD8+
T14613 26348-26349 NN denotes T
T14614 26350-26355 NN denotes cells
T14615 26357-26361 JJ denotes CD8+
T14616 26362-26363 NN denotes T
T14617 26364-26369 NN denotes cells
T14618 26370-26374 IN denotes from
T14619 26375-26385 JJ denotes 4–8-wk-old
T14620 26386-26393 NN denotes Tcra−/−
T14621 26394-26395 NN denotes ×
T14622 26396-26399 NN denotes P14
T14623 26400-26403 NN denotes TCR
T14624 26404-26414 JJ denotes transgenic
T14625 26415-26416 -LRB- denotes (
T14626 26416-26423 JJ denotes Taconic
T14627 26423-26424 -RRB- denotes )
T14628 26424-26425 -COMMA- denotes ,
T14629 26426-26434 NN denotes C57BL/6J
T14630 26435-26437 NNP denotes WT
T14631 26437-26438 -COMMA- denotes ,
T14632 26439-26441 CC denotes or
T14633 26442-26450 NN denotes Tbx21−/−
T14634 26451-26452 -LRB- denotes (
T14635 26452-26455 DT denotes The
T14636 26456-26463 NNP denotes Jackson
T14637 26464-26474 NNP denotes Laboratory
T14638 26474-26475 -RRB- denotes )
T14639 26476-26480 NN denotes mice
T14640 26481-26485 VB denotes were
T14641 26486-26494 VB denotes purified
T14642 26495-26496 -LRB- denotes (
T14643 26496-26497 SYM denotes >
T14644 26497-26499 CD denotes 95
T14645 26499-26500 NN denotes %
T14646 26501-26507 NN denotes purity
T14647 26507-26508 -RRB- denotes )
T14648 26509-26511 IN denotes by
T14649 26512-26520 JJ denotes negative
T14650 26521-26530 NN denotes selection
T14651 26531-26532 -LRB- denotes (
T14652 26532-26542 NN denotes Invitrogen
T14653 26542-26543 -RRB- denotes )
T14654 26544-26548 IN denotes from
T14655 26549-26555 VB denotes pooled
T14656 26556-26562 NN denotes spleen
T14657 26563-26566 CC denotes and
T14658 26567-26572 NN denotes lymph
T14659 26573-26577 NN denotes node
T14660 26578-26583 NN denotes cells
T14661 26585-26589 JJ denotes CD8+
T14662 26590-26591 NN denotes T
T14663 26592-26597 NN denotes cells
T14664 26598-26602 IN denotes from
T14665 26603-26611 NN denotes Runx3−/−
T14666 26612-26616 NN denotes mice
T14667 26617-26619 IN denotes on
T14668 26620-26623 DT denotes the
T14669 26624-26627 NN denotes ICR
T14670 26628-26638 NN denotes background
T14671 26639-26643 VB denotes were
T14672 26644-26652 VB denotes purified
T14673 26653-26655 IN denotes by
T14674 26656-26664 JJ denotes positive
T14675 26665-26674 NN denotes selection
T14676 26675-26676 -LRB- denotes (
T14677 26676-26684 NNP denotes Miltenyi
T14678 26685-26691 NNP denotes Biotec
T14679 26691-26692 -RRB- denotes )
T14680 26694-26697 DT denotes All
T14681 26698-26702 NN denotes mice
T14682 26703-26707 VB denotes were
T14683 26708-26718 VB denotes maintained
T14684 26719-26721 IN denotes in
T14685 26722-26730 JJ denotes specific
T14686 26731-26744 JJ denotes pathogen-free
T14687 26745-26752 NN denotes barrier
T14688 26753-26763 NN denotes facilities
T14689 26764-26767 CC denotes and
T14690 26768-26772 VB denotes used
T14691 26773-26782 VB denotes according
T14692 26783-26785 TO denotes to
T14693 26786-26795 NN denotes protocols
T14694 26796-26804 VB denotes approved
T14695 26805-26807 IN denotes by
T14696 26808-26811 DT denotes the
T14697 26812-26818 NNP denotes Immune
T14698 26819-26826 NNP denotes Disease
T14699 26827-26836 NNP denotes Institute
T14700 26837-26840 CC denotes and
T14701 26841-26844 DT denotes the
T14702 26845-26852 NNP denotes Harvard
T14703 26853-26860 NNP denotes Medical
T14704 26861-26867 NNP denotes School
T14705 26868-26874 NNP denotes Animal
T14706 26875-26879 NNP denotes Care
T14707 26880-26883 CC denotes and
T14708 26884-26887 NNP denotes Use
T14709 26888-26899 NNP denotes Committees.
T14710 26900-26903 IN denotes For
T14711 26904-26915 NN denotes stimulation
T14712 26915-26916 -COMMA- denotes ,
T14713 26917-26925 VB denotes purified
T14714 26926-26930 JJ denotes CD8+
T14715 26931-26932 NN denotes T
T14716 26933-26938 NN denotes cells
T14717 26939-26943 VB denotes were
T14718 26944-26952 VB denotes cultured
T14719 26953-26955 IN denotes at
T14720 26956-26959 CD denotes 106
T14721 26960-26968 NN denotes cells/ml
T14722 26969-26970 -LRB- denotes (
T14723 26970-26972 CD denotes 10
T14724 26973-26975 NN denotes ml
T14725 26975-26976 -RRB- denotes )
T14726 26977-26979 IN denotes in
T14727 26980-26983 NN denotes T25
T14728 26984-26990 NN denotes flasks
T14729 26991-26997 VB denotes coated
T14730 26998-27002 IN denotes with
T14731 27003-27004 CD denotes 1
T14732 27005-27010 NN denotes μg/ml
T14733 27011-27015 DT denotes each
T14734 27016-27018 IN denotes of
T14735 27019-27027 NN denotes anti-CD3
T14736 27028-27029 -LRB- denotes (
T14737 27029-27034 NN denotes clone
T14738 27035-27039 NN denotes 2C11
T14739 27039-27040 -RRB- denotes )
T14740 27041-27044 CC denotes and
T14741 27045-27054 JJ denotes anti-CD28
T14742 27055-27056 -LRB- denotes (
T14743 27056-27061 NN denotes clone
T14744 27062-27067 CD denotes 37.51
T14745 27067-27068 -RRB- denotes )
T14746 27069-27071 IN denotes by
T14747 27072-27084 NN denotes pretreatment
T14748 27085-27089 IN denotes with
T14749 27090-27093 CD denotes 300
T14750 27094-27099 NN denotes μg/ml
T14751 27100-27104 NN denotes goat
T14752 27105-27117 NN denotes anti–hamster
T14753 27118-27121 NN denotes IgG
T14754 27123-27128 IN denotes After
T14755 27129-27131 CD denotes 48
T14756 27132-27133 NN denotes h
T14757 27133-27134 -COMMA- denotes ,
T14758 27135-27140 NN denotes cells
T14759 27141-27145 VB denotes were
T14760 27146-27153 VB denotes removed
T14761 27154-27158 IN denotes from
T14762 27159-27162 DT denotes the
T14763 27163-27166 NN denotes TCR
T14764 27167-27178 NN denotes stimulation
T14765 27179-27182 CC denotes and
T14766 27183-27193 VB denotes recultured
T14767 27194-27196 IN denotes at
T14768 27197-27198 DT denotes a
T14769 27199-27212 NN denotes concentration
T14770 27213-27215 IN denotes of
T14771 27216-27217 CD denotes 5
T14772 27218-27219 IN denotes ×
T14773 27220-27223 CD denotes 105
T14774 27224-27232 NN denotes cells/ml
T14775 27233-27235 IN denotes in
T14776 27236-27241 NN denotes media
T14777 27242-27254 VB denotes supplemented
T14778 27255-27259 IN denotes with
T14779 27260-27263 CD denotes 100
T14780 27264-27268 NN denotes U/ml
T14781 27269-27275 NN denotes rhIL-2
T14782 27277-27282 DT denotes Every
T14783 27283-27285 CD denotes 24
T14784 27286-27287 NN denotes h
T14785 27287-27288 -COMMA- denotes ,
T14786 27289-27295 JJ denotes viable
T14787 27296-27301 NN denotes cells
T14788 27302-27306 VB denotes were
T14789 27307-27314 VB denotes counted
T14790 27315-27318 CC denotes and
T14791 27319-27329 VB denotes readjusted
T14792 27330-27332 TO denotes to
T14793 27333-27334 CD denotes 5
T14794 27335-27336 IN denotes ×
T14795 27337-27340 CD denotes 105
T14796 27341-27349 NN denotes cells/ml
T14797 27350-27354 IN denotes with
T14798 27355-27360 JJ denotes fresh
T14799 27361-27366 NN denotes media
T14800 27367-27377 VB denotes containing
T14801 27378-27381 DT denotes the
T14802 27382-27395 VB denotes corresponding
T14803 27396-27402 NN denotes amount
T14804 27403-27405 IN denotes of
T14805 27406-27412 NN denotes rhIL-2
T15411 27415-27424 NN denotes Isolation
T15412 27425-27427 IN denotes of
T15413 27428-27432 JJ denotes CD8+
T15414 27433-27434 NN denotes T
T15415 27435-27440 NN denotes cells
T15416 27441-27445 IN denotes from
T15417 27446-27454 NN denotes Runx3−/−
T15418 27455-27459 NN denotes mice
T15419 27461-27476 JJ denotes Runx3-deficient
T15420 27477-27478 NN denotes T
T15421 27479-27484 NN denotes cells
T15422 27485-27489 VB denotes fail
T15423 27490-27492 TO denotes to
T15424 27493-27500 NN denotes silence
T15425 27501-27504 NN denotes CD4
T15426 27505-27515 NN denotes expression
T15427 27516-27524 RB denotes normally
T15428 27525-27526 -LRB- denotes (
T15429 27526-27530 NNP denotes Fig.
T15430 27531-27533 NN denotes S1
T15431 27533-27534 -RRB- denotes )
T15432 27535-27536 -LRB- denotes (
T15433 27536-27538 CD denotes 12
T15434 27538-27539 -COMMA- denotes ,
T15435 27540-27542 CD denotes 13
T15436 27542-27543 -RRB- denotes )
T15437 27545-27547 PRP denotes We
T15438 27548-27557 RB denotes therefore
T15439 27558-27565 RB denotes further
T15440 27566-27578 VB denotes fractionated
T15441 27579-27582 DT denotes the
T15442 27583-27593 RB denotes positively
T15443 27594-27602 VB denotes selected
T15444 27603-27607 JJ denotes CD8+
T15445 27608-27609 NN denotes T
T15446 27610-27615 NN denotes cells
T15447 27616-27620 IN denotes from
T15448 27621-27626 NN denotes Runx3
T15449 27627-27629 NN denotes KO
T15450 27630-27634 NN denotes mice
T15451 27635-27639 IN denotes into
T15452 27640-27648 NN denotes CD8+CD4−
T15453 27649-27651 NN denotes SP
T15454 27652-27654 CC denotes or
T15455 27655-27663 JJ denotes CD8+CD4+
T15456 27664-27666 NN denotes DP
T15457 27667-27672 NN denotes cells
T15458 27673-27675 IN denotes by
T15459 27676-27686 NN denotes separation
T15460 27687-27692 VB denotes using
T15461 27693-27701 JJ denotes anti-CD4
T15462 27702-27710 JJ denotes magnetic
T15463 27711-27716 NN denotes beads
T15464 27718-27722 DT denotes This
T15465 27723-27730 VB denotes yielded
T15466 27731-27732 DT denotes a
T15467 27733-27738 NN denotes Runx3
T15468 27739-27741 NN denotes KO
T15469 27742-27744 NN denotes SP
T15470 27745-27755 VB denotes “enriched”
T15471 27756-27766 NN denotes population
T15472 27767-27771 WDT denotes that
T15473 27772-27781 VB denotes contained
T15474 27782-27784 CD denotes 75
T15475 27784-27785 NN denotes %
T15476 27786-27794 NN denotes CD8+CD4−
T15477 27795-27800 NN denotes cells
T15478 27801-27804 CC denotes and
T15479 27805-27806 DT denotes a
T15480 27807-27809 NN denotes KO
T15481 27810-27812 NN denotes DP
T15482 27813-27821 VB denotes enriched
T15483 27822-27832 NN denotes population
T15484 27833-27837 WDT denotes that
T15485 27838-27847 VB denotes contained
T15486 27848-27850 CD denotes 85
T15487 27850-27851 NN denotes %
T15488 27852-27860 JJ denotes CD8+CD4+
T15489 27861-27866 NN denotes cells
T15490 27867-27868 -LRB- denotes (
T15491 27868-27872 NN denotes Fig.
T15492 27873-27875 NN denotes S1
T15493 27875-27876 -RRB- denotes )
T15494 27878-27881 DT denotes The
T15495 27882-27887 NN denotes cells
T15496 27888-27892 VB denotes were
T15497 27893-27903 VB denotes stimulated
T15498 27904-27908 IN denotes with
T15499 27909-27918 JJ denotes anti-CD3+
T15500 27919-27928 NN denotes anti-CD28
T15501 27929-27932 IN denotes for
T15502 27933-27934 CD denotes 2
T15503 27935-27936 NN denotes d
T15504 27937-27943 IN denotes before
T15505 27944-27952 VB denotes removing
T15506 27953-27957 PRP denotes them
T15507 27958-27962 IN denotes from
T15508 27963-27966 DT denotes the
T15509 27967-27970 NN denotes TCR
T15510 27971-27979 NN denotes stimulus
T15511 27980-27983 CC denotes and
T15512 27984-27993 VB denotes culturing
T15513 27994-27998 PRP denotes them
T15514 27999-28001 IN denotes in
T15515 28002-28007 NN denotes media
T15516 28008-28018 VB denotes containing
T15517 28019-28022 CD denotes 100
T15518 28023-28027 NN denotes U/ml
T15519 28028-28032 NN denotes IL-2
T15520 28034-28036 IN denotes As
T15521 28037-28047 RB denotes previously
T15522 28048-28056 VB denotes reported
T15523 28056-28057 -COMMA- denotes ,
T15524 28058-28069 JJ denotes TCR-induced
T15525 28070-28083 NN denotes proliferation
T15526 28084-28086 IN denotes of
T15527 28087-28095 NN denotes Runx3−/−
T15528 28096-28100 JJ denotes CD8+
T15529 28101-28102 NN denotes T
T15530 28103-28108 NN denotes cells
T15531 28109-28112 VB denotes was
T15532 28113-28121 RB denotes severely
T15533 28122-28130 JJ denotes impaired
T15534 28130-28131 -COMMA- denotes ,
T15535 28132-28144 JJ denotes irrespective
T15536 28145-28147 IN denotes of
T15537 28148-28151 NN denotes CD4
T15538 28152-28162 NN denotes expression
T15539 28163-28164 -LRB- denotes (
T15540 28164-28168 NN denotes Fig.
T15541 28169-28171 NN denotes S1
T15542 28171-28172 -RRB- denotes )
T15543 28173-28174 -LRB- denotes (
T15544 28174-28176 CD denotes 12
T15545 28176-28177 -COMMA- denotes ,
T15546 28178-28180 CD denotes 13
T15547 28180-28181 -RRB- denotes )
T15548 28183-28190 RB denotes However
T15549 28190-28191 -COMMA- denotes ,
T15550 28192-28195 DT denotes the
T15551 28196-28204 NN denotes Runx3−/−
T15552 28205-28210 NN denotes cells
T15553 28211-28217 VB denotes showed
T15554 28218-28230 JJ denotes cell-surface
T15555 28231-28241 NN denotes expression
T15556 28242-28250 NN denotes patterns
T15557 28251-28261 JJ denotes indicative
T15558 28262-28264 IN denotes of
T15559 28265-28274 VB denotes activated
T15560 28275-28280 NN denotes cells
T15561 28280-28281 -COMMA- denotes ,
T15562 28282-28291 VB denotes including
T15563 28292-28305 NN denotes up-regulation
T15564 28306-28308 IN denotes of
T15565 28309-28313 NN denotes CD25
T15566 28314-28317 CC denotes and
T15567 28318-28322 NN denotes CD69
T15568 28323-28324 -LRB- denotes (
T15569 28324-28328 NN denotes Fig.
T15570 28329-28331 NN denotes S1
T15571 28331-28332 -RRB- denotes )
T15572 28334-28336 IN denotes As
T15573 28337-28345 VB denotes expected
T15574 28346-28350 IN denotes from
T15575 28351-28356 PRP-DOLLAR- denotes their
T15576 28357-28364 NN denotes ability
T15577 28365-28367 TO denotes to
T15578 28368-28379 VB denotes up-regulate
T15579 28380-28384 NN denotes CD25
T15580 28384-28385 -COMMA- denotes ,
T15581 28386-28394 NN denotes Runx3−/−
T15582 28395-28399 JJ denotes CD8+
T15583 28400-28401 NN denotes T
T15584 28402-28407 NN denotes cells
T15585 28408-28417 VB denotes responded
T15586 28418-28420 TO denotes to
T15587 28421-28425 NN denotes IL-2
T15588 28426-28441 NN denotes supplementation
T15589 28442-28447 IN denotes after
T15590 28448-28451 NN denotes day
T15591 28452-28453 CD denotes 2
T15592 28454-28457 CC denotes and
T15593 28458-28469 RB denotes efficiently
T15594 28470-28478 VB denotes expanded
T15595 28479-28484 IN denotes until
T15596 28485-28488 NN denotes day
T15597 28489-28490 CD denotes 6
T15598 28491-28493 IN denotes of
T15599 28494-28497 DT denotes the
T15600 28498-28505 NN denotes culture
T15601 28506-28512 NN denotes period
T15602 28512-28513 -COMMA- denotes ,
T15603 28514-28520 IN denotes albeit
T15604 28521-28523 IN denotes at
T15605 28524-28530 JJ denotes slower
T15606 28531-28536 NN denotes rates
T15607 28537-28545 VB denotes compared
T15608 28546-28550 IN denotes with
T15609 28551-28553 JJ denotes WT
T15610 28554-28559 NN denotes cells
T15611 28560-28561 -LRB- denotes (
T15612 28561-28565 NN denotes Fig.
T15613 28566-28568 NN denotes S1
T15614 28568-28569 -RRB- denotes )
T15615 28571-28579 IN denotes Although
T15616 28580-28581 DT denotes a
T15617 28582-28590 NN denotes fraction
T15618 28591-28593 IN denotes of
T15619 28594-28597 DT denotes the
T15620 28598-28600 NN denotes KO
T15621 28601-28603 NN denotes DP
T15622 28604-28609 NN denotes cells
T15623 28610-28618 VB denotes silenced
T15624 28619-28622 NN denotes CD4
T15625 28623-28633 NN denotes expression
T15626 28634-28639 IN denotes after
T15627 28640-28650 NN denotes activation
T15628 28650-28651 -COMMA- denotes ,
T15629 28652-28655 DT denotes the
T15630 28656-28661 NN denotes ratio
T15631 28662-28664 IN denotes of
T15632 28665-28670 NN denotes SP/DP
T15633 28671-28676 NN denotes cells
T15634 28677-28679 IN denotes in
T15635 28680-28684 DT denotes each
T15636 28685-28693 VB denotes enriched
T15637 28694-28704 NN denotes population
T15638 28705-28713 VB denotes remained
T15639 28714-28722 JJ denotes constant
T15640 28723-28733 RB denotes thereafter
T15641 28733-28734 -COMMA- denotes ,
T15642 28735-28738 CC denotes and
T15643 28739-28741 PRP denotes we
T15644 28742-28745 VB denotes did
T15645 28746-28749 RB denotes not
T15646 28750-28757 VB denotes observe
T15647 28758-28761 DT denotes any
T15648 28762-28767 JJ denotes major
T15649 28768-28779 NN denotes differences
T15650 28780-28787 IN denotes between
T15651 28788-28793 DT denotes these
T15652 28794-28797 CD denotes two
T15653 28798-28809 NN denotes populations
T15654 28810-28820 IN denotes throughout
T15655 28821-28824 DT denotes the
T15656 28825-28832 NN denotes culture
T15657 28833-28839 NN denotes period
T15658 28839-28840 -COMMA- denotes ,
T15659 28841-28851 VB denotes indicating
T15660 28852-28856 IN denotes that
T15661 28857-28859 IN denotes in
T15662 28860-28865 NN denotes terms
T15663 28866-28868 IN denotes of
T15664 28869-28877 NN denotes effector
T15665 28878-28881 NN denotes CTL
T15666 28882-28897 NN denotes differentiation
T15667 28898-28901 CC denotes and
T15668 28902-28907 IN denotes under
T15669 28908-28911 PRP-DOLLAR- denotes our
T15670 28912-28919 NN denotes culture
T15671 28920-28930 NN denotes conditions
T15672 28930-28931 -COMMA- denotes ,
T15673 28932-28940 NN denotes Runx3−/−
T15674 28941-28945 JJ denotes CD8+
T15675 28946-28947 NN denotes T
T15676 28948-28953 NN denotes cells
T15677 28954-28958 WDT denotes that
T15678 28959-28963 RB denotes also
T15679 28964-28973 VB denotes coexpress
T15680 28974-28977 NN denotes CD4
T15681 28978-28981 VB denotes are
T15682 28982-28999 JJ denotes indistinguishable
T15683 29000-29004 IN denotes from
T15684 29005-29010 DT denotes those
T15685 29011-29015 WDT denotes that
T15686 29016-29018 VB denotes do
T15687 29019-29022 RB denotes not
T15688 29024-29027 DT denotes The
T15689 29028-29032 NN denotes data
T15690 29033-29042 VB denotes presented
T15691 29043-29045 IN denotes in
T15692 29046-29050 NNP denotes Fig.
T15693 29051-29053 NN denotes S2
T15694 29054-29057 VB denotes are
T15695 29058-29062 IN denotes from
T15696 29063-29068 NN denotes Runx3
T15697 29069-29071 NN denotes KO
T15698 29072-29074 NN denotes SP
T15699 29075-29080 NN denotes cells
T15700 29080-29081 -COMMA- denotes ,
T15701 29082-29089 IN denotes whereas
T15702 29090-29095 DT denotes those
T15703 29096-29101 VB denotes shown
T15704 29102-29104 IN denotes in
T15705 29105-29110 NNP denotes Figs.
T15706 29111-29112 CD denotes 3
T15707 29113-29116 CC denotes and
T15708 29117-29118 CD denotes 4
T15709 29119-29122 VB denotes are
T15710 29123-29127 IN denotes from
T15711 29128-29133 JJ denotes total
T15712 29134-29139 NN denotes Runx3
T15713 29140-29142 NN denotes KO
T15714 29143-29146 NN denotes CD8
T15715 29147-29152 NN denotes cells
T16229 29155-29165 JJ denotes FACS-based
T16230 29166-29178 NN denotes cytotoxicity
T16231 29179-29184 NN denotes assay
T16232 29186-29188 TO denotes To
T16233 29189-29196 VB denotes measure
T16234 29197-29209 NN denotes cytotoxicity
T16235 29209-29210 -COMMA- denotes ,
T16236 29211-29214 NN denotes EL4
T16237 29215-29222 NN denotes thymoma
T16238 29223-29229 NN denotes target
T16239 29230-29235 NN denotes cells
T16240 29236-29240 VB denotes were
T16241 29241-29247 VB denotes loaded
T16242 29248-29252 IN denotes with
T16243 29253-29254 CD denotes 0
T16244 29255-29257 CC denotes or
T16245 29258-29259 CD denotes 1
T16246 29260-29262 NN denotes μM
T16247 29263-29267 NN denotes Gp33
T16248 29268-29275 NN denotes peptide
T16249 29276-29279 IN denotes for
T16250 29280-29281 CD denotes 2
T16251 29282-29283 NN denotes h
T16252 29284-29290 IN denotes before
T16253 29291-29292 DT denotes a
T16254 29293-29296 JJ denotes 2-h
T16255 29297-29309 NN denotes coincubation
T16256 29310-29314 IN denotes with
T16257 29315-29318 NN denotes P14
T16258 29319-29323 JJ denotes CD8+
T16259 29324-29325 NN denotes T
T16260 29326-29331 NN denotes cells
T16261 29332-29334 IN denotes at
T16262 29335-29338 DT denotes the
T16263 29339-29357 JJ denotes effector-to-target
T16264 29358-29364 NN denotes ratios
T16265 29365-29374 VB denotes indicated
T16266 29375-29377 IN denotes in
T16267 29378-29381 DT denotes the
T16268 29382-29389 NN denotes figures
T16269 29390-29392 IN denotes in
T16270 29393-29400 JJ denotes 96-well
T16271 29401-29413 JJ denotes round-bottom
T16272 29414-29420 NN denotes plates
T16273 29422-29427 IN denotes After
T16274 29428-29431 DT denotes the
T16275 29432-29444 NN denotes coincubation
T16276 29445-29451 NN denotes period
T16277 29451-29452 -COMMA- denotes ,
T16278 29453-29458 NN denotes cells
T16279 29459-29463 VB denotes were
T16280 29464-29471 VB denotes stained
T16281 29472-29476 IN denotes with
T16282 29477-29484 NN denotes Annexin
T16283 29485-29491 NN denotes V–FITC
T16284 29492-29495 CC denotes and
T16285 29496-29520 NN denotes anti-CD8–allophycocyanin
T16286 29522-29526 NN denotes Data
T16287 29527-29535 NN denotes analysis
T16288 29536-29539 VB denotes was
T16289 29540-29549 VB denotes performed
T16290 29550-29554 IN denotes with
T16291 29555-29561 NN denotes FlowJo
T16292 29562-29570 NN denotes software
T16293 29571-29572 -LRB- denotes (
T16294 29572-29576 NNP denotes Tree
T16295 29577-29581 NNP denotes Star
T16296 29581-29582 -COMMA- denotes ,
T16297 29583-29587 NNP denotes Inc.
T16298 29587-29588 -RRB- denotes )
T16299 29588-29589 -COLON- denotes ;
T16300 29590-29593 NN denotes EL4
T16301 29594-29600 NN denotes target
T16302 29601-29606 NN denotes cells
T16303 29607-29608 -LRB- denotes (
T16304 29608-29620 JJ denotes CD8-negative
T16305 29621-29627 NN denotes events
T16306 29627-29628 -RRB- denotes )
T16307 29629-29633 VB denotes were
T16308 29634-29639 VB denotes gated
T16309 29639-29640 -COMMA- denotes ,
T16310 29641-29644 CC denotes and
T16311 29645-29648 DT denotes the
T16312 29649-29659 NN denotes percentage
T16313 29660-29662 IN denotes of
T16314 29663-29670 NN denotes Annexin
T16315 29671-29673 JJ denotes V+
T16316 29674-29680 NN denotes target
T16317 29681-29686 NN denotes cells
T16318 29687-29690 VB denotes was
T16319 29691-29701 VB denotes determined
T16510 29704-29712 NN denotes Cytokine
T16511 29713-29716 CC denotes and
T16512 29717-29724 NN denotes surface
T16513 29725-29731 NN denotes marker
T16514 29732-29740 NN denotes staining
T16515 29742-29744 TO denotes To
T16516 29745-29751 VB denotes assess
T16517 29752-29760 NN denotes cytokine
T16518 29761-29771 NN denotes production
T16519 29771-29772 -COMMA- denotes ,
T16520 29773-29778 NN denotes cells
T16521 29779-29783 VB denotes were
T16522 29784-29796 VB denotes restimulated
T16523 29797-29801 IN denotes with
T16524 29802-29804 CD denotes 10
T16525 29805-29807 NN denotes nM
T16526 29808-29811 NN denotes PMA
T16527 29812-29813 SYM denotes +
T16528 29814-29815 CD denotes 1
T16529 29816-29818 NN denotes μM
T16530 29819-29828 NN denotes ionomycin
T16531 29829-29832 IN denotes for
T16532 29833-29834 CD denotes 6
T16533 29835-29836 NN denotes h
T16534 29837-29838 -LRB- denotes (
T16535 29838-29844 IN denotes unless
T16536 29845-29854 VB denotes indicated
T16537 29855-29864 RB denotes otherwise
T16538 29865-29867 IN denotes in
T16539 29868-29871 DT denotes the
T16540 29872-29879 NN denotes figures
T16541 29879-29880 -RRB- denotes )
T16542 29880-29881 -COMMA- denotes ,
T16543 29882-29885 CC denotes and
T16544 29886-29899 JJ denotes intracellular
T16545 29900-29908 NN denotes cytokine
T16546 29909-29915 NN denotes stains
T16547 29916-29920 VB denotes were
T16548 29921-29930 VB denotes performed
T16549 29931-29933 IN denotes as
T16550 29934-29944 RB denotes previously
T16551 29945-29954 VB denotes described
T16552 29955-29956 -LRB- denotes (
T16553 29956-29958 CD denotes 28
T16554 29958-29959 -RRB- denotes )
T16555 29961-29963 TO denotes To
T16556 29964-29970 VB denotes detect
T16557 29971-29981 NN denotes expression
T16558 29982-29984 IN denotes of
T16559 29985-29992 NN denotes surface
T16560 29993-30002 NN denotes molecules
T16561 30002-30003 -COMMA- denotes ,
T16562 30004-30009 NN denotes cells
T16563 30010-30014 VB denotes were
T16564 30015-30021 VB denotes washed
T16565 30022-30024 IN denotes in
T16566 30025-30028 NNP denotes PBS
T16567 30028-30029 -COMMA- denotes ,
T16568 30030-30041 VB denotes resuspended
T16569 30042-30044 IN denotes in
T16570 30045-30049 NN denotes FACS
T16571 30050-30054 NN denotes wash
T16572 30055-30061 NN denotes buffer
T16573 30062-30063 -LRB- denotes (
T16574 30063-30064 CD denotes 3
T16575 30064-30065 NN denotes %
T16576 30066-30069 NN denotes FBS
T16577 30069-30070 -COMMA- denotes ,
T16578 30071-30074 CD denotes 0.1
T16579 30074-30075 NN denotes %
T16580 30076-30082 NN denotes sodium
T16581 30083-30088 NN denotes azide
T16582 30088-30089 -COMMA- denotes ,
T16583 30090-30092 CD denotes 30
T16584 30093-30095 NN denotes mM
T16585 30096-30101 NNP denotes Hepes
T16586 30101-30102 -COMMA- denotes ,
T16587 30103-30105 CD denotes 1×
T16588 30106-30109 NNP denotes PBS
T16589 30109-30110 -RRB- denotes )
T16590 30111-30121 VB denotes containing
T16591 30122-30125 DT denotes the
T16592 30126-30136 NN denotes antibodies
T16593 30137-30146 VB denotes indicated
T16594 30147-30149 IN denotes in
T16595 30150-30153 DT denotes the
T16596 30154-30161 NN denotes figures
T16597 30162-30164 IN denotes at
T16609 30243-30244 -LRB- denotes (
T16610 30244-30246 NN denotes RT
T16611 30246-30247 -RRB- denotes )
T16612 30247-30248 -COMMA- denotes ,
T16613 30249-30255 VB denotes washed
T16614 30255-30256 -COMMA- denotes ,
T16615 30257-30260 CC denotes and
T16616 30261-30272 VB denotes resuspended
T16617 30273-30275 IN denotes in
T16618 30276-30277 CD denotes 2
T16619 30277-30278 NN denotes %
T16620 30279-30291 NN denotes formaldehyde
T16621 30292-30300 JJ denotes fixative
T16622 30301-30309 NN denotes solution
T16623 30310-30316 IN denotes before
T16624 30317-30328 NN denotes acquisition
T16625 30329-30331 IN denotes on
T16626 30332-30333 DT denotes a
T16627 30334-30345 NN denotes FACSCalibur
T16628 30346-30347 -LRB- denotes (
T16629 30347-30349 NN denotes BD
T16630 30349-30350 -RRB- denotes )
T16785 30353-30363 JJ denotes Retroviral
T16786 30364-30376 NN denotes transduction
T16787 30377-30379 IN denotes of
T16788 30380-30387 JJ denotes primary
T16789 30388-30392 JJ denotes CD8+
T16790 30393-30394 NN denotes T
T16791 30395-30400 NN denotes cells
T16792 30402-30405 IN denotes For
T16793 30406-30418 NN denotes transduction
T16794 30419-30430 NN denotes experiments
T16795 30430-30431 -COMMA- denotes ,
T16796 30432-30437 JJ denotes viral
T16797 30438-30450 NN denotes supernatants
T16798 30451-30455 VB denotes were
T16799 30456-30465 VB denotes generated
T16800 30466-30468 IN denotes by
T16801 30469-30476 NN denotes calcium
T16802 30477-30486 NN denotes phosphate
T16803 30487-30499 NN denotes transfection
T16804 30500-30502 IN denotes of
T16805 30503-30510 NN denotes Phoenix
T16806 30511-30516 NN denotes cells
T16807 30517-30520 CC denotes and
T16808 30521-30534 NN denotes concentration
T16809 30535-30537 IN denotes by
T16810 30538-30547 JJ denotes overnight
T16811 30548-30562 NN denotes centrifugation
T16812 30563-30565 IN denotes at
T16813 30566-30571 CD denotes 6,000
T16814 30572-30573 NN denotes g
T16815 30575-30577 IN denotes At
T16816 30578-30581 CD denotes ∼42
T16817 30582-30583 NN denotes h
T16818 30584-30589 IN denotes after
T16819 30590-30593 DT denotes the
T16820 30594-30601 JJ denotes initial
T16821 30602-30605 NN denotes TCR
T16822 30606-30616 NN denotes activation
T16823 30617-30619 IN denotes of
T16824 30620-30623 CD denotes 106
T16825 30624-30628 JJ denotes CD8+
T16826 30629-30630 NN denotes T
T16827 30631-30636 NN denotes cells
T16828 30637-30640 IN denotes per
T16829 30641-30645 RB denotes well
T16830 30646-30648 IN denotes in
T16831 30649-30656 JJ denotes 12-well
T16832 30657-30663 NN denotes plates
T16833 30663-30664 -COMMA- denotes ,
T16834 30665-30668 DT denotes the
T16835 30669-30676 NN denotes culture
T16836 30677-30682 NN denotes media
T16837 30683-30686 VB denotes was
T16838 30687-30694 VB denotes removed
T16839 30695-30698 CC denotes and
T16840 30699-30707 VB denotes replaced
T16841 30708-30712 IN denotes with
T16842 30713-30721 JJ denotes complete
T16843 30722-30727 NN denotes media
T16844 30728-30740 VB denotes supplemented
T16845 30741-30745 IN denotes with
T16846 30746-30747 CD denotes 8
T16847 30748-30753 NN denotes μg/ml
T16848 30754-30763 NN denotes polybrene
T16849 30764-30774 VB denotes containing
T16850 30775-30780 JJ denotes fresh
T16851 30781-30785 CC denotes plus
T16852 30786-30798 JJ denotes concentrated
T16853 30799-30804 NN denotes virus
T16854 30806-30809 DT denotes The
T16855 30810-30816 NN denotes plates
T16856 30817-30821 VB denotes were
T16857 30822-30833 VB denotes centrifuged
T16858 30834-30836 IN denotes at
T16859 30837-30840 CD denotes 700
T16860 30841-30842 NN denotes g
T16861 30843-30846 IN denotes for
T16862 30847-30848 CD denotes 1
T16863 30849-30850 NN denotes h
T16864 30851-30853 IN denotes at
T16865 30854-30856 NN denotes RT
T16866 30857-30863 IN denotes before
T16867 30864-30873 VB denotes returning
T16868 30874-30876 TO denotes to
T16869 30877-30881 NN denotes 37°C
T16870 30882-30885 IN denotes for
T16871 30886-30888 DT denotes an
T16872 30889-30899 JJ denotes additional
T16873 30900-30901 CD denotes 5
T16874 30902-30903 NN denotes h
T16875 30905-30915 JJ denotes Retroviral
T16876 30916-30926 NN denotes constructs
T16877 30927-30930 IN denotes for
T16878 30931-30941 NN denotes Eomes-VP16
T16879 30942-30945 CC denotes and
T16880 30946-30949 DT denotes the
T16881 30950-30953 NN denotes MIG
T16882 30954-30961 NN denotes control
T16883 30962-30967 JJ denotes empty
T16884 30968-30974 NN denotes vector
T16885 30975-30979 VB denotes were
T16886 30980-30981 DT denotes a
T16887 30982-30986 NN denotes gift
T16888 30987-30991 IN denotes from
T16889 30992-30996 NNP denotes S.L.
T16890 30997-31003 NNP denotes Reiner
T16891 31004-31005 -LRB- denotes (
T16892 31005-31015 NNP denotes University
T16893 31016-31018 IN denotes of
T16894 31019-31031 NNP denotes Pennsylvania
T16895 31031-31032 -COMMA- denotes ,
T16896 31033-31045 NNP denotes Philadelphia
T16897 31045-31046 -COMMA- denotes ,
T16898 31047-31049 NNP denotes PA
T16899 31049-31050 -RRB- denotes )
T16900 31051-31052 -LRB- denotes (
T16901 31052-31053 CD denotes 8
T16902 31053-31054 -RRB- denotes )
T16963 31057-31061 NN denotes ChIP
T16964 31062-31065 CC denotes and
T16965 31066-31075 JJ denotes real-time
T16966 31076-31079 NN denotes PCR
T16967 31080-31088 NN denotes analysis
T16968 31090-31092 CD denotes 20
T16969 31093-31094 CC denotes ×
T16970 31095-31098 CD denotes 106
T16971 31099-31103 JJ denotes CD8+
T16972 31104-31105 NN denotes T
T16973 31106-31111 NN denotes cells
T16974 31112-31115 IN denotes per
T16975 31116-31135 NN denotes immunoprecipitation
T16976 31136-31140 VB denotes were
T16977 31141-31146 VB denotes fixed
T16978 31147-31149 IN denotes by
T16979 31150-31156 VB denotes adding
T16980 31157-31158 DT denotes a
T16981 31159-31165 JJ denotes 1/10th
T16982 31166-31172 NN denotes volume
T16983 31173-31175 IN denotes of
T16984 31176-31184 NN denotes fixation
T16985 31185-31193 NN denotes solution
T16986 31194-31195 -LRB- denotes (
T16987 31195-31199 CD denotes 11.1
T16988 31199-31200 NN denotes %
T16989 31201-31213 NN denotes formaldehyde
T16990 31213-31214 -COMMA- denotes ,
T16991 31215-31218 CD denotes 100
T16992 31219-31221 NN denotes mM
T16993 31222-31226 NN denotes NaCl
T16994 31226-31227 -COMMA- denotes ,
T16995 31228-31229 CD denotes 1
T16996 31230-31232 NN denotes mM
T16997 31233-31237 NN denotes EDTA
T16998 31237-31238 -COMMA- denotes ,
T16999 31239-31242 CD denotes 0.5
T17000 31243-31245 NN denotes mM
T17001 31246-31250 NN denotes EGTA
T17002 31250-31251 -COMMA- denotes ,
T17003 31252-31254 CD denotes 50
T17004 31255-31257 NN denotes mM
T17005 31258-31263 NN denotes Hepes
T17006 31263-31264 -RRB- denotes )
T17007 31265-31267 TO denotes to
T17008 31268-31269 CD denotes 1
T17009 31270-31276 NN denotes volume
T17010 31277-31279 IN denotes of
T17011 31280-31287 NN denotes culture
T17012 31288-31293 NN denotes media
T17013 31294-31297 CC denotes and
T17014 31298-31302 VB denotes were
T17015 31303-31312 VB denotes incubated
T17016 31313-31316 IN denotes for
T17017 31317-31319 CD denotes 10
T17018 31320-31322 CC denotes or
T17019 31323-31325 CD denotes 30
T17020 31326-31329 NN denotes min
T17021 31330-31332 IN denotes at
T17022 31333-31335 NN denotes RT
T17023 31337-31345 NN denotes Fixation
T17024 31346-31349 VB denotes was
T17025 31350-31357 VB denotes stopped
T17026 31358-31362 IN denotes with
T17027 31363-31366 CD denotes 120
T17028 31367-31369 NN denotes mM
T17029 31370-31377 NN denotes glycine
T17030 31378-31380 IN denotes on
T17031 31381-31384 NN denotes ice
T17032 31385-31388 IN denotes for
T17033 31389-31390 CD denotes 5
T17034 31391-31394 NN denotes min
T17035 31396-31401 VB denotes Fixed
T17036 31402-31407 NN denotes cells
T17037 31408-31412 VB denotes were
T17038 31413-31419 VB denotes washed
T17039 31420-31422 CD denotes 2×
T17040 31423-31427 IN denotes with
T17041 31428-31432 JJ denotes cold
T17042 31433-31436 NNP denotes PBS
T17043 31436-31437 -COMMA- denotes ,
T17044 31438-31440 CD denotes 1×
T17045 31441-31445 IN denotes with
T17046 31446-31450 JJ denotes cold
T17047 31451-31459 NN denotes solution
T17048 31460-31461 CD denotes I
T17049 31462-31463 -LRB- denotes (
T17050 31463-31465 CD denotes 10
T17051 31466-31468 NN denotes mM
T17052 31469-31473 NN denotes Tris
T17053 31474-31475 -LRB- denotes [
T17054 31475-31477 NN denotes pH
T17055 31478-31481 CD denotes 7.5
T17056 31481-31482 -RRB- denotes ]
T17057 31482-31483 -COMMA- denotes ,
T17058 31484-31486 CD denotes 10
T17059 31487-31489 NN denotes mM
T17060 31490-31494 NN denotes EDTA
T17061 31494-31495 -COMMA- denotes ,
T17062 31496-31499 CD denotes 0.5
T17063 31500-31502 NN denotes mM
T17064 31503-31507 NN denotes EGTA
T17065 31507-31508 -COMMA- denotes ,
T17066 31509-31510 CD denotes 1
T17067 31510-31511 NN denotes %
T17068 31512-31518 NN denotes Triton
T17069 31519-31524 NN denotes X-100
T17070 31524-31525 -RRB- denotes )
T17071 31525-31526 -COMMA- denotes ,
T17072 31527-31530 CC denotes and
T17073 31531-31533 CD denotes 1×
T17074 31534-31538 IN denotes with
T17075 31539-31543 JJ denotes cold
T17076 31544-31552 NN denotes solution
T17077 31553-31555 CD denotes II
T17078 31556-31557 -LRB- denotes (
T17079 31557-31559 CD denotes 10
T17080 31560-31562 NN denotes mM
T17081 31563-31567 NN denotes Tris
T17082 31568-31569 -LRB- denotes [
T17083 31569-31571 NN denotes pH
T17084 31572-31575 CD denotes 7.5
T17085 31575-31576 -RRB- denotes ]
T17086 31576-31577 -COMMA- denotes ,
T17087 31578-31579 CD denotes 1
T17088 31580-31582 NN denotes mM
T17089 31583-31587 NN denotes EDTA
T17090 31587-31588 -COMMA- denotes ,
T17091 31589-31592 CD denotes 0.5
T17092 31593-31595 NN denotes mM
T17093 31596-31600 NN denotes EGTA
T17094 31600-31601 -COMMA- denotes ,
T17095 31602-31605 CD denotes 200
T17096 31606-31608 NN denotes mM
T17097 31609-31613 NN denotes NaCl
T17098 31613-31614 -RRB- denotes )
T17099 31616-31621 IN denotes After
T17100 31622-31628 NN denotes washes
T17101 31628-31629 -COMMA- denotes ,
T17102 31630-31634 NN denotes cell
T17103 31635-31642 NN denotes pellets
T17104 31643-31647 VB denotes were
T17105 31648-31659 VB denotes resuspended
T17106 31660-31662 IN denotes at
T17107 31663-31665 CD denotes 40
T17108 31666-31667 CC denotes ×
T17109 31668-31671 CD denotes 106
T17110 31672-31680 NN denotes cells/ml
T17111 31681-31683 IN denotes in
T17112 31684-31688 NN denotes ChIP
T17113 31689-31694 NN denotes lysis
T17114 31695-31701 NN denotes buffer
T17115 31702-31703 -LRB- denotes (
T17116 31703-31706 CD denotes 150
T17117 31707-31709 NN denotes mM
T17118 31710-31714 NN denotes NaCl
T17119 31714-31715 -COMMA- denotes ,
T17120 31716-31718 CD denotes 25
T17121 31719-31721 NN denotes mM
T17122 31722-31726 NN denotes Tris
T17123 31727-31728 -LRB- denotes [
T17124 31728-31730 NN denotes pH
T17125 31731-31734 CD denotes 7.5
T17126 31734-31735 -RRB- denotes ]
T17127 31735-31736 -COMMA- denotes ,
T17128 31737-31738 CD denotes 1
T17129 31738-31739 NN denotes %
T17130 31740-31746 NN denotes Triton
T17131 31747-31752 NN denotes X-100
T17132 31752-31753 -COMMA- denotes ,
T17133 31754-31757 CD denotes 0.1
T17134 31757-31758 NN denotes %
T17135 31759-31762 NN denotes SDS
T17136 31762-31763 -COMMA- denotes ,
T17137 31764-31767 CD denotes 0.5
T17138 31767-31768 NN denotes %
T17139 31769-31781 NN denotes deoxycholate
T17140 31782-31786 CC denotes plus
T17141 31787-31795 NN denotes protease
T17142 31796-31799 CC denotes and
T17143 31800-31811 NN denotes phosphatase
T17144 31812-31822 NN denotes inhibitors
T17145 31822-31823 -RRB- denotes )
T17146 31823-31824 -COMMA- denotes ,
T17147 31825-31828 CC denotes and
T17148 31829-31838 NN denotes chromatin
T17149 31839-31842 VB denotes was
T17150 31843-31850 VB denotes sheared
T17151 31851-31855 IN denotes with
T17152 31856-31857 DT denotes a
T17153 31858-31867 NN denotes sonicator
T17154 31868-31870 TO denotes to
T17155 31871-31876 VB denotes yield
T17156 31877-31885 JJ denotes 0.5–1-kb
T17157 31886-31889 NN denotes DNA
T17158 31890-31899 NN denotes fragments
T17159 31901-31906 IN denotes After
T17160 31907-31918 VB denotes preclearing
T17161 31919-31922 DT denotes the
T17162 31923-31930 JJ denotes sheared
T17163 31931-31940 NN denotes chromatin
T17164 31941-31945 IN denotes with
T17165 31946-31953 NN denotes protein
T17166 31954-31965 NN denotes A–sepharose
T17167 31966-31971 NN denotes beads
T17168 31972-31975 CC denotes and
T17169 31976-31984 VB denotes removing
T17170 31985-31986 CD denotes 5
T17171 31986-31987 NN denotes %
T17172 31988-31990 IN denotes as
T17173 31991-31996 NN denotes input
T17174 31997-32006 NN denotes chromatin
T17175 32006-32007 -COMMA- denotes ,
T17176 32008-32027 NN denotes immunoprecipitation
T17177 32028-32031 VB denotes was
T17178 32032-32041 VB denotes performed
T17179 32042-32044 IN denotes by
T17180 32045-32051 VB denotes adding
T17181 32052-32061 VB denotes optimized
T17182 32062-32070 NN denotes antibody
T17183 32071-32078 NN denotes amounts
T17184 32079-32080 -LRB- denotes (
T17185 32080-32083 IN denotes per
T17186 32084-32086 CD denotes 20
T17187 32087-32088 -COLON- denotes ×
T17188 32089-32092 CD denotes 106
T17189 32093-32097 NN denotes cell
T17190 32098-32109 NN denotes equivalents
T17191 32109-32110 -COLON- denotes :
T17192 32111-32114 CD denotes 2.5
T17193 32115-32117 NN denotes μg
T17194 32118-32128 NN denotes anti-Eomes
T17195 32128-32129 -COMMA- denotes ,
T17196 32130-32131 CD denotes 1
T17197 32131-32132 SYM denotes :
T17198 32132-32135 CD denotes 100
T17199 32136-32144 NN denotes dilution
T17200 32145-32155 NN denotes anti-Runx3
T17201 32155-32156 -RRB- denotes )
T17202 32156-32157 -COMMA- denotes ,
T17203 32158-32166 VB denotes followed
T17204 32167-32169 IN denotes by
T17205 32170-32179 JJ denotes overnight
T17206 32180-32190 NN denotes incubation
T17207 32191-32193 IN denotes at
T17208 32194-32197 NN denotes 4°C
T17209 32197-32198 -COLON- denotes ;
T17210 32199-32206 NN denotes protein
T17211 32207-32218 NN denotes A–sepharose
T17212 32219-32224 NN denotes beads
T17213 32225-32229 VB denotes were
T17214 32230-32235 VB denotes added
T17215 32236-32239 IN denotes for
T17216 32240-32243 DT denotes the
T17217 32244-32248 JJ denotes last
T17218 32249-32250 CD denotes 3
T17219 32251-32252 NN denotes h
T17220 32253-32255 IN denotes of
T17221 32256-32259 DT denotes the
T17222 32260-32270 NN denotes incubation
T17223 32271-32277 NN denotes period
T17224 32279-32284 NN denotes Beads
T17225 32285-32289 VB denotes were
T17226 32290-32296 VB denotes washed
T17227 32297-32299 CD denotes 2×
T17228 32300-32304 IN denotes with
T17229 32305-32309 NN denotes RIPA
T17230 32310-32316 NN denotes buffer
T17231 32317-32318 -LRB- denotes (
T17232 32318-32320 CD denotes 50
T17233 32321-32323 NN denotes mM
T17234 32324-32328 NN denotes Tris
T17235 32329-32330 -LRB- denotes [
T17236 32330-32332 NN denotes pH
T17237 32333-32334 CD denotes 8
T17238 32334-32335 -RRB- denotes ]
T17239 32335-32336 -COMMA- denotes ,
T17240 32337-32340 CD denotes 150
T17241 32341-32343 NN denotes mM
T17242 32344-32348 NN denotes NaCl
T17243 32348-32349 -COMMA- denotes ,
T17244 32350-32351 CD denotes 1
T17245 32352-32354 NN denotes mM
T17246 32355-32359 NN denotes EDTA
T17247 32359-32360 -COMMA- denotes ,
T17248 32361-32362 CD denotes 1
T17249 32362-32363 NN denotes %
T17250 32364-32369 NN denotes NP-40
T17251 32369-32370 -COMMA- denotes ,
T17252 32371-32374 CD denotes 0.1
T17253 32374-32375 NN denotes %
T17254 32376-32379 NN denotes SDS
T17255 32379-32380 -COMMA- denotes ,
T17256 32381-32384 CD denotes 0.5
T17257 32384-32385 NN denotes %
T17258 32386-32398 NN denotes deoxycholate
T17259 32398-32399 -RRB- denotes )
T17260 32399-32400 -COMMA- denotes ,
T17261 32401-32403 CD denotes 1×
T17262 32404-32408 IN denotes with
T17263 32409-32413 JJ denotes high
T17264 32414-32418 NN denotes salt
T17265 32419-32425 NN denotes buffer
T17266 32426-32427 -LRB- denotes (
T17267 32427-32429 CD denotes 50
T17268 32430-32432 NN denotes mM
T17269 32433-32437 NN denotes Tris
T17270 32438-32439 -LRB- denotes [
T17271 32439-32441 NN denotes pH
T17272 32442-32443 CD denotes 8
T17273 32443-32444 -RRB- denotes ]
T17274 32444-32445 -COMMA- denotes ,
T17275 32446-32449 CD denotes 500
T17276 32450-32452 NN denotes mM
T17277 32453-32457 NN denotes NaCl
T17278 32457-32458 -COMMA- denotes ,
T17279 32459-32460 CD denotes 1
T17280 32461-32463 NN denotes mM
T17281 32464-32468 NN denotes EDTA
T17282 32468-32469 -COMMA- denotes ,
T17283 32470-32471 CD denotes 1
T17284 32471-32472 NN denotes %
T17285 32473-32478 NN denotes NP-40
T17286 32478-32479 -COMMA- denotes ,
T17287 32480-32483 CD denotes 0.1
T17288 32483-32484 NN denotes %
T17289 32485-32488 NN denotes SDS
T17290 32488-32489 -RRB- denotes )
T17291 32489-32490 -COMMA- denotes ,
T17292 32491-32494 CC denotes and
T17293 32495-32497 NN denotes 1×
T17294 32498-32502 IN denotes with
T17295 32503-32505 NN denotes TE
T17296 32506-32512 NN denotes buffer
T17297 32514-32519 IN denotes After
T17298 32520-32523 DT denotes the
T17299 32524-32528 JJ denotes last
T17300 32529-32533 NN denotes wash
T17301 32533-32534 -COMMA- denotes ,
T17302 32535-32538 NN denotes DNA
T17303 32539-32542 VB denotes was
T17304 32543-32549 VB denotes eluted
T17305 32550-32552 IN denotes by
T17306 32553-32565 VB denotes resuspending
T17307 32566-32569 DT denotes the
T17308 32570-32575 NN denotes beads
T17309 32576-32578 IN denotes in
T17310 32579-32586 NN denotes elution
T17311 32587-32593 NN denotes buffer
T17312 32594-32595 -LRB- denotes (
T17313 32595-32596 CD denotes 1
T17314 32596-32597 NN denotes %
T17315 32598-32601 NN denotes SDS
T17316 32601-32602 -COMMA- denotes ,
T17317 32603-32606 CD denotes 100
T17318 32607-32609 NN denotes mM
T17319 32610-32616 NN denotes NaHCO3
T17320 32616-32617 -RRB- denotes )
T17321 32619-32623 CC denotes Both
T17322 32624-32629 NN denotes input
T17323 32630-32633 CC denotes and
T17324 32634-32638 NN denotes ChIP
T17325 32639-32648 NN denotes chromatin
T17326 32649-32653 VB denotes were
T17327 32654-32658 RB denotes then
T17328 32659-32666 VB denotes treated
T17329 32667-32671 IN denotes with
T17330 32672-32677 NN denotes RNase
T17331 32678-32679 NN denotes A
T17332 32680-32681 -LRB- denotes (
T17333 32681-32682 CD denotes 5
T17334 32683-32685 NN denotes μg
T17335 32686-32691 NN denotes total
T17336 32691-32692 -RRB- denotes )
T17337 32693-32696 IN denotes for
T17338 32697-32698 CD denotes 1
T17339 32699-32700 NN denotes h
T17340 32701-32703 IN denotes at
T17341 32704-32708 NN denotes 37°C
T17342 32708-32709 -COMMA- denotes ,
T17343 32710-32718 VB denotes followed
T17344 32719-32721 IN denotes by
T17345 32722-32725 DT denotes the
T17346 32726-32734 NN denotes addition
T17347 32735-32737 IN denotes of
T17348 32738-32748 NN denotes proteinase
T17349 32749-32750 NN denotes K
T17350 32751-32752 -LRB- denotes (
T17351 32752-32755 CD denotes 100
T17352 32756-32758 NN denotes μg
T17353 32759-32764 NN denotes total
T17354 32764-32765 -RRB- denotes )
T17355 32766-32769 CC denotes and
T17356 32770-32779 JJ denotes overnight
T17357 32780-32790 NN denotes incubation
T17358 32791-32793 IN denotes at
T17359 32794-32798 NN denotes 65°C
T17360 32799-32801 TO denotes to
T17361 32802-32809 VB denotes reverse
T17362 32810-32823 NN denotes cross-linking
T17363 32825-32828 NN denotes DNA
T17364 32829-32832 VB denotes was
T17365 32833-32837 RB denotes then
T17366 32838-32846 VB denotes purified
T17367 32847-32851 IN denotes with
T17368 32852-32860 JJ denotes QIAquick
T17369 32861-32868 NN denotes columns
T17370 32869-32870 -LRB- denotes (
T17371 32870-32873 NN denotes Gel
T17372 32874-32884 NN denotes Extraction
T17373 32885-32888 NN denotes Kit
T17374 32888-32889 -COLON- denotes ;
T17375 32890-32896 NN denotes QIAGEN
T17376 32896-32897 -RRB- denotes )
T17377 32898-32907 VB denotes according
T17378 32908-32910 TO denotes to
T17379 32911-32914 DT denotes the
T17380 32915-32927 NN denotes manufacturer
T17381 32927-32929 POS denotes 's
T17382 32930-32942 NN denotes instructions
T17383 32943-32946 CC denotes and
T17384 32947-32958 VB denotes resuspended
T17385 32959-32961 IN denotes in
T17386 32962-32963 DT denotes a
T17387 32964-32969 JJ denotes 50-μl
T17388 32970-32976 NN denotes volume
T17389 32978-32981 IN denotes For
T17390 32982-32991 JJ denotes real-time
T17391 32992-32995 NN denotes PCR
T17392 32996-33005 NN denotes detection
T17393 33006-33008 IN denotes of
T17394 33009-33027 JJ denotes immunoprecipitated
T17395 33028-33035 NN denotes targets
T17396 33036-33041 VB denotes using
T17397 33042-33045 DT denotes the
T17398 33046-33050 NNP denotes SYBR
T17399 33051-33056 NNP denotes Green
T17400 33057-33060 NN denotes PCR
T17401 33061-33064 NN denotes Kit
T17402 33064-33065 -COMMA- denotes ,
T17403 33066-33067 DT denotes a
T17404 33068-33076 JJ denotes standard
T17405 33077-33082 NN denotes curve
T17406 33083-33086 VB denotes was
T17407 33087-33095 VB denotes obtained
T17408 33096-33100 IN denotes with
T17409 33101-33107 JJ denotes serial
T17410 33108-33117 NN denotes dilutions
T17411 33118-33120 IN denotes of
T17412 33121-33126 NN denotes input
T17413 33127-33130 NN denotes DNA
T17414 33131-33134 IN denotes for
T17415 33135-33139 DT denotes each
T17416 33140-33146 NN denotes sample
T17417 33146-33147 -COMMA- denotes ,
T17418 33148-33151 CC denotes and
T17419 33152-33153 CD denotes 1
T17420 33154-33156 NN denotes μl
T17421 33157-33161 NN denotes ChIP
T17422 33162-33165 NN denotes DNA
T17423 33166-33169 VB denotes was
T17424 33170-33174 VB denotes used
T17425 33175-33178 IN denotes per
T17426 33179-33182 NN denotes PCR
T17427 33183-33191 NN denotes reaction
T17428 33192-33193 -LRB- denotes (
T17429 33193-33202 VB denotes performed
T17430 33203-33205 IN denotes in
T17431 33206-33216 NN denotes duplicates
T17432 33216-33217 -RRB- denotes )
T17433 33219-33223 NN denotes Melt
T17434 33224-33230 NN denotes curves
T17435 33231-33234 CC denotes and
T17436 33235-33242 NN denotes agarose
T17437 33243-33247 NN denotes gels
T17438 33248-33252 VB denotes were
T17439 33253-33261 VB denotes analyzed
T17440 33262-33264 TO denotes to
T17441 33265-33271 VB denotes ensure
T17442 33272-33285 NN denotes amplification
T17443 33286-33288 IN denotes of
T17444 33289-33297 JJ denotes specific
T17445 33298-33304 NN denotes target
T17446 33305-33314 NN denotes sequences
T17447 33316-33321 VB denotes Refer
T17448 33322-33324 TO denotes to
T17449 33325-33330 JJ denotes Table
T17450 33331-33333 NN denotes S1
T17451 33334-33335 -LRB- denotes (
T17452 33335-33344 JJ denotes available
T17453 33345-33347 IN denotes at
T17454 33348-33352 NN denotes http
T17455 33352-33353 -COLON- denotes :
T17456 33353-33400 NN denotes //www.jem.org/cgi/content/full/jem.20081242/DC1
T17457 33400-33401 -RRB- denotes )
T17458 33402-33405 IN denotes for
T17459 33406-33407 DT denotes a
T17460 33408-33412 NN denotes list
T17461 33413-33415 IN denotes of
T17462 33416-33422 NN denotes primer
T17463 33423-33427 NN denotes sets
T17464 33429-33432 DT denotes The
T17465 33433-33437 NN denotes data
T17466 33438-33441 VB denotes are
T17467 33442-33451 VB denotes presented
T17468 33452-33454 IN denotes as
T17469 33455-33458 DT denotes the
T17470 33459-33465 NN denotes number
T17471 33466-33468 IN denotes of
T17472 33469-33487 VB denotes immunoprecipitated
T17473 33488-33494 NN denotes target
T17474 33495-33504 NN denotes sequences
T17475 33505-33513 JJ denotes relative
T17476 33514-33516 TO denotes to
T17477 33517-33522 NN denotes input
T17478 33523-33532 NN denotes chromatin
T17479 33532-33533 -COMMA- denotes ,
T17480 33534-33542 VB denotes assuming
T17481 33543-33546 CD denotes two
T17482 33547-33553 NN denotes copies
T17483 33554-33556 IN denotes of
T17484 33557-33563 NN denotes target
T17485 33564-33572 NN denotes sequence
T17486 33573-33576 IN denotes per
T17487 33577-33581 NN denotes cell
T17488 33582-33592 JJ denotes equivalent
T17489 33593-33597 VB denotes used
T17490 33598-33601 IN denotes for
T17491 33602-33605 DT denotes the
T17492 33606-33610 NN denotes ChIP
T17850 33742-33743 -COMMA- denotes ,
T17851 33744-33746 CD denotes 10
T17852 33747-33749 NN denotes μg
T17853 33750-33752 IN denotes of
T17854 33753-33758 JJ denotes total
T17855 33759-33762 NN denotes RNA
T17856 33763-33766 VB denotes was
T17857 33767-33773 VB denotes loaded
T17858 33774-33777 IN denotes per
T17859 33778-33782 NN denotes lane
T17860 33783-33786 CC denotes and
T17861 33787-33798 VB denotes transferred
T17862 33799-33801 TO denotes to
T17863 33802-33812 RB denotes positively
T17864 33813-33820 VB denotes charged
T17865 33821-33826 NN denotes nylon
T17866 33827-33836 NN denotes membranes
T17867 33837-33838 -LRB- denotes (
T17868 33838-33847 JJ denotes Hybond-N+
T17869 33847-33848 -COLON- denotes ;
T17870 33849-33851 NNP denotes GE
T17871 33852-33862 NNP denotes Healthcare
T17872 33862-33863 -RRB- denotes )
T17873 33863-33864 -COMMA- denotes ,
T17874 33865-33870 WDT denotes which
T17875 33871-33874 VB denotes was
T17876 33875-33884 VB denotes confirmed
T17877 33885-33887 IN denotes by
T17878 33888-33896 NN denotes ethidium
T17879 33897-33904 NN denotes bromide
T17880 33905-33913 NN denotes staining
T17881 33914-33916 IN denotes of
T17882 33917-33926 JJ denotes ribosomal
T17883 33927-33930 NN denotes RNA
T17884 33931-33938 NN denotes species
T17885 33939-33941 IN denotes on
T17886 33942-33945 DT denotes the
T17887 33946-33954 NN denotes membrane
T17888 33956-33965 NN denotes Membranes
T17889 33966-33970 VB denotes were
T17890 33971-33981 VB denotes hybridized
T17891 33982-33986 IN denotes with
T17892 33987-33988 CD denotes 1
T17893 33989-33994 NN denotes ng/ml
T17894 33995-33997 SYM denotes α-
T17895 33997-33998 -LRB- denotes [
T17896 33998-34001 NN denotes 32P
T17897 34001-34002 -RRB- denotes ]
T17898 34002-34014 VB denotes dCTP–labeled
T17899 34015-34030 JJ denotes trichloroacetic
T17900 34031-34035 NN denotes acid
T17901 34036-34048 JJ denotes precipitable
T17902 34049-34054 NN denotes probe
T17903 34055-34057 IN denotes in
T17904 34058-34068 NN denotes ExpressHyb
T17905 34069-34082 NN denotes hybridization
T17906 34083-34089 NN denotes buffer
T17907 34090-34091 -LRB- denotes (
T17908 34091-34099 NNP denotes Clontech
T17909 34100-34112 NNP denotes Laboratories
T17910 34112-34113 -COMMA- denotes ,
T17911 34114-34118 NNP denotes Inc.
T17912 34118-34119 -RRB- denotes )
T17913 34121-34124 DT denotes All
T17914 34125-34129 NN denotes cDNA
T17915 34130-34136 NN denotes probes
T17916 34137-34141 VB denotes were
T17917 34142-34151 VB denotes confirmed
T17918 34152-34154 TO denotes to
T17919 34155-34159 VB denotes have
T17920 34160-34163 DT denotes the
T17921 34164-34175 JJ denotes appropriate
T17922 34176-34187 JJ denotes single-copy
T17923 34188-34199 NN denotes specificity
T17924 34200-34205 IN denotes under
T17925 34206-34211 DT denotes these
T17926 34212-34222 NN denotes conditions
T17927 34223-34228 VB denotes using
T17928 34229-34236 JJ denotes genomic
T17929 34237-34245 JJ denotes Southern
T17930 34246-34250 NN denotes blot
T17931 34251-34259 NN denotes analysis
T17932 34261-34265 NN denotes Band
T17933 34266-34277 NN denotes intensities
T17934 34278-34282 VB denotes were
T17935 34283-34291 VB denotes acquired
T17936 34292-34294 IN denotes by
T17937 34295-34310 VB denotes phosphorimaging
T17938 34311-34319 NN denotes analysis
T17939 34321-34324 IN denotes For
T17940 34325-34332 JJ denotes Western
T17941 34333-34341 NN denotes analysis
T17942 34341-34342 -COMMA- denotes ,
T17943 34343-34353 JJ denotes whole-cell
T17944 34354-34361 NN denotes protein
T17945 34362-34369 NN denotes lysates
T17946 34370-34374 VB denotes were
T17947 34375-34383 VB denotes obtained
T17948 34384-34388 IN denotes from
T17949 34389-34393 JJ denotes CD8+
T17950 34394-34395 NN denotes T
T17951 34396-34401 NN denotes cells
T17952 34402-34404 IN denotes at
T17953 34405-34408 DT denotes the
T17954 34409-34413 NN denotes time
T17955 34414-34420 NN denotes points
T17956 34421-34430 VB denotes indicated
T17957 34431-34433 IN denotes in
T17958 34434-34437 DT denotes the
T17959 34438-34445 NN denotes figures
T17960 34446-34452 IN denotes during
T17961 34453-34459 JJ denotes clonal
T17962 34460-34469 NN denotes expansion
T17963 34470-34472 IN denotes in
T17964 34473-34476 CD denotes 100
T17965 34477-34481 NN denotes U/ml
T17966 34482-34486 NN denotes IL-2
T17967 34487-34491 IN denotes with
T17968 34492-34497 NN denotes lysis
T17969 34498-34504 NN denotes buffer
T17970 34505-34506 -LRB- denotes (
T17971 34506-34508 CD denotes 50
T17972 34509-34511 NN denotes mM
T17973 34512-34516 NN denotes Tris
T17974 34517-34518 -LRB- denotes [
T17975 34518-34520 NN denotes pH
T17976 34521-34524 CD denotes 7.5
T17977 34524-34525 -RRB- denotes ]
T17978 34525-34526 -COMMA- denotes ,
T17979 34527-34530 CD denotes 150
T17980 34531-34533 NN denotes mM
T17981 34534-34538 NN denotes NaCl
T17982 34538-34539 -COMMA- denotes ,
T17983 34540-34542 CD denotes 10
T17984 34542-34543 NN denotes %
T17985 34544-34552 NN denotes glycerol
T17986 34552-34553 -COMMA- denotes ,
T17987 34554-34555 CD denotes 5
T17988 34556-34558 NN denotes mM
T17989 34559-34563 NN denotes EDTA
T17990 34563-34564 -COMMA- denotes ,
T17991 34565-34566 CD denotes 1
T17992 34566-34567 NN denotes %
T17993 34568-34573 NN denotes NP-40
T17994 34573-34574 -RRB- denotes )
T17995 34575-34577 IN denotes by
T17996 34578-34590 VB denotes resuspending
T17997 34591-34598 NN denotes samples
T17998 34599-34601 IN denotes in
T17999 34602-34604 CD denotes 10
T18000 34605-34607 NN denotes μl
T18001 34608-34611 IN denotes per
T18002 34612-34615 CD denotes 106
T18003 34616-34621 NN denotes cells
T18004 34622-34625 CC denotes and
T18005 34626-34636 VB denotes incubating
T18006 34637-34639 IN denotes on
T18007 34640-34643 NN denotes ice
T18008 34644-34647 IN denotes for
T18009 34648-34650 CD denotes 30
T18010 34651-34654 NN denotes min
T18011 34655-34657 IN denotes in
T18012 34658-34661 DT denotes the
T18013 34662-34670 NN denotes presence
T18014 34671-34673 IN denotes of
T18015 34674-34682 NN denotes protease
T18016 34683-34693 NN denotes inhibitors
T18017 34695-34705 NN denotes Immunoblot
T18018 34706-34714 NN denotes analysis
T18019 34715-34718 VB denotes was
T18020 34719-34728 VB denotes performed
T18021 34729-34733 IN denotes with
T18022 34734-34737 DT denotes the
T18023 34738-34748 NN denotes antibodies
T18024 34749-34758 VB denotes indicated
T18025 34759-34761 IN denotes in
T18026 34762-34765 DT denotes the
T18027 34766-34773 NN denotes figures
T18028 34774-34779 IN denotes after
T18029 34780-34788 NN denotes SDS-PAGE
T18030 34789-34790 -LRB- denotes (
T18031 34790-34795 CD denotes 10–30
T18032 34796-34798 NN denotes μg
T18033 34799-34801 IN denotes of
T18034 34802-34807 JJ denotes total
T18035 34808-34815 NN denotes protein
T18036 34816-34819 VB denotes was
T18037 34820-34826 VB denotes loaded
T18038 34827-34830 IN denotes per
T18039 34831-34835 RB denotes well
T18040 34835-34836 -RRB- denotes )
T18041 34838-34852 NN denotes Quantification
T18042 34853-34855 IN denotes of
T18043 34856-34864 VB denotes detected
T18044 34865-34872 NN denotes protein
T18045 34873-34876 VB denotes was
T18046 34877-34886 VB denotes performed
T18047 34887-34891 IN denotes with
T18048 34892-34894 DT denotes an
T18049 34895-34906 NNP denotes Intelligent
T18050 34907-34911 NNP denotes Dark
T18051 34912-34915 NNP denotes Box
T18052 34916-34920 NN denotes unit
T18053 34921-34922 -LRB- denotes (
T18054 34922-34930 NN denotes LAS-3000
T18055 34930-34931 -COLON- denotes ;
T18056 34932-34940 NNP denotes Fujifilm
T18057 34940-34941 -RRB- denotes )
T18058 34942-34945 CC denotes and
T18059 34946-34956 VB denotes normalized
T18060 34957-34960 IN denotes for
T18061 34961-34968 VB denotes loading
T18062 34969-34973 IN denotes with
T18063 34974-34977 DT denotes the
T18064 34978-34984 NN denotes amount
T18065 34985-34987 IN denotes of
T18066 34988-34991 NN denotes RNA
T18067 34992-34998 NN denotes Pol-II
T18068 34999-35007 VB denotes detected
T18069 35008-35010 IN denotes in
T18070 35011-35015 DT denotes each
T18071 35016-35020 NN denotes lane
T18190 35023-35029 NN denotes Online
T18191 35030-35042 JJ denotes supplemental
T18192 35043-35051 NN denotes material
T18193 35053-35057 NN denotes Fig.
T18194 35058-35060 NN denotes S1
T18195 35061-35066 VB denotes shows
T18196 35067-35070 DT denotes the
T18197 35071-35087 NN denotes characterization
T18198 35088-35090 IN denotes of
T18199 35091-35101 JJ denotes peripheral
T18200 35102-35106 JJ denotes CD8+
T18201 35107-35108 NN denotes T
T18202 35109-35114 NN denotes cells
T18203 35115-35119 IN denotes from
T18204 35120-35128 NN denotes Runx3−/−
T18205 35129-35133 NN denotes mice
T18206 35135-35139 NN denotes Fig.
T18207 35140-35142 NN denotes S2
T18208 35143-35148 VB denotes shows
T18209 35149-35157 NN denotes effector
T18210 35158-35165 NN denotes protein
T18211 35166-35176 NN denotes expression
T18212 35177-35179 IN denotes by
T18213 35180-35185 NN denotes Runx3
T18214 35186-35188 NN denotes WT
T18215 35189-35192 CC denotes and
T18216 35193-35195 NN denotes KO
T18217 35196-35201 NN denotes cells
T18218 35202-35204 IN denotes at
T18219 35205-35208 NN denotes day
T18220 35209-35210 CD denotes 4
T18221 35211-35213 IN denotes of
T18222 35214-35216 FW denotes in
T18223 35217-35222 FW denotes vitro
T18224 35223-35230 NN denotes culture
T18225 35232-35238 NN denotes Primer
T18226 35239-35248 NN denotes sequences
T18227 35249-35253 VB denotes used
T18228 35254-35257 IN denotes for
T18229 35258-35262 NN denotes ChIP
T18230 35263-35274 NN denotes experiments
T18231 35275-35278 VB denotes are
T18232 35279-35284 VB denotes shown
T18233 35285-35287 IN denotes in
T18234 35288-35293 JJ denotes Table
T18235 35294-35296 NN denotes S1
T18236 35298-35304 NN denotes Online
T18237 35305-35317 JJ denotes supplemental
T18238 35318-35326 NN denotes material
T18239 35327-35329 VB denotes is
T18240 35330-35339 JJ denotes available
T18241 35340-35342 IN denotes at
T18242 35343-35347 NN denotes http
T18243 35347-35348 -COLON- denotes :
T18244 35348-35395 NN denotes //www.jem.org/cgi/content/full/jem.20081242/DC1
T18624 6546-6554 NN denotes Kinetics
T18625 6555-6557 IN denotes of
T18626 6558-6562 NN denotes gene
T18627 6563-6573 NN denotes expression
T18628 6574-6580 IN denotes during
T18629 6581-6585 JJ denotes CD8+
T18630 6586-6587 NN denotes T
T18631 6588-6592 NN denotes cell
T18632 6593-6609 NN denotes differentiation.
T18633 6610-6611 -LRB- denotes (
T18634 6611-6612 NN denotes A
T18635 6612-6613 -RRB- denotes )
T18636 6614-6622 NN denotes Kinetics
T18637 6623-6625 IN denotes of
T18638 6626-6630 NN denotes Prf1
T18639 6630-6631 -COMMA- denotes ,
T18640 6632-6636 NN denotes Gzmb
T18641 6636-6637 -COMMA- denotes ,
T18642 6638-6643 NN denotes Tbx21
T18643 6644-6645 -LRB- denotes (
T18644 6645-6650 NN denotes T-bet
T18645 6650-6651 -RRB- denotes )
T18646 6651-6652 -COMMA- denotes ,
T18647 6653-6656 CC denotes and
T18648 6657-6662 NN denotes Eomes
T18649 6663-6667 NN denotes mRNA
T18650 6668-6678 NN denotes expression
T18651 6679-6681 IN denotes in
T18652 6682-6697 VB denotes differentiating
T18653 6698-6701 NN denotes P14
T18654 6702-6706 JJ denotes CD8+
T18655 6707-6708 NN denotes T
T18656 6709-6714 NN denotes cells
T18657 6715-6723 VB denotes analyzed
T18658 6724-6726 IN denotes by
T18659 6727-6735 JJ denotes Northern
T18660 6736-6744 NN denotes blotting
T18661 6746-6749 NN denotes RNA
T18662 6750-6754 IN denotes from
T18663 6755-6758 NN denotes day
T18664 6759-6760 CD denotes 7
T18665 6761-6764 NN denotes Th1
T18666 6765-6770 NN denotes cells
T18667 6771-6774 VB denotes was
T18668 6775-6779 VB denotes used
T18669 6780-6782 IN denotes as
T18670 6783-6784 DT denotes a
T18671 6785-6792 NN denotes control
T18672 6794-6799 NN denotes Sizes
T18673 6800-6802 IN denotes of
T18674 6803-6807 NN denotes mRNA
T18675 6808-6819 NN denotes transcripts
T18676 6820-6823 VB denotes are
T18677 6824-6834 VB denotes indicated.
T18678 6835-6836 -LRB- denotes (
T18679 6836-6837 NN denotes B
T18680 6837-6838 -RRB- denotes )
T18681 6839-6853 NN denotes Quantification
T18682 6854-6856 IN denotes of
T18683 6857-6865 JJ denotes relative
T18684 6866-6870 NN denotes mRNA
T18685 6871-6878 NN denotes amounts
T18686 6879-6881 IN denotes by
T18687 6882-6896 NN denotes phosphorimager
T18688 6897-6906 NN denotes analysis.
T18689 6907-6908 -LRB- denotes (
T18690 6908-6909 NN denotes C
T18691 6909-6910 -RRB- denotes )
T18692 6911-6919 NN denotes Kinetics
T18693 6920-6922 IN denotes of
T18694 6923-6930 NN denotes protein
T18695 6931-6941 NN denotes expression
T18696 6942-6944 IN denotes in
T18697 6945-6960 VB denotes differentiating
T18698 6961-6964 NN denotes P14
T18699 6965-6969 JJ denotes CD8+
T18700 6970-6971 NN denotes T
T18701 6972-6977 NN denotes cells
T18702 6978-6986 VB denotes analyzed
T18703 6987-6989 IN denotes by
T18704 6990-7004 NN denotes immunoblotting
T18705 7006-7011 NN denotes Sizes
T18706 7012-7014 IN denotes of
T18707 7015-7022 NN denotes protein
T18708 7023-7028 NN denotes bands
T18709 7029-7032 VB denotes are
T18710 7033-7043 VB denotes indicated.
T18711 7044-7045 -LRB- denotes (
T18712 7045-7046 NN denotes D
T18713 7046-7047 -RRB- denotes )
T18714 7048-7056 JJ denotes Relative
T18715 7057-7064 NN denotes protein
T18716 7065-7072 NN denotes amounts
T18717 7073-7083 VB denotes quantified
T18718 7084-7088 IN denotes from
T18719 7089-7092 DT denotes the
T18720 7093-7100 JJ denotes Western
T18721 7101-7107 NN denotes blots.
T18722 7108-7109 -LRB- denotes (
T18723 7109-7110 NN denotes E
T18724 7110-7111 -RRB- denotes )
T18725 7112-7125 JJ denotes Intracellular
T18726 7126-7134 NN denotes staining
T18727 7135-7138 IN denotes for
T18728 7139-7147 NN denotes granzyme
T18729 7148-7149 NN denotes B
T18730 7149-7150 -COMMA- denotes ,
T18731 7151-7156 NN denotes IFN-γ
T18732 7156-7157 -COMMA- denotes ,
T18733 7158-7161 CC denotes and
T18734 7162-7165 NN denotes TNF
T18735 7167-7175 NN denotes Granzyme
T18736 7176-7177 NN denotes B
T18737 7178-7186 NN denotes staining
T18738 7187-7190 VB denotes was
T18739 7191-7199 JJ denotes specific
T18740 7200-7208 JJ denotes relative
T18741 7209-7211 TO denotes to
T18742 7212-7214 DT denotes an
T18743 7215-7222 NN denotes isotype
T18744 7223-7230 NN denotes control
T18745 7231-7232 -LRB- denotes (
T18746 7232-7235 RB denotes not
T18747 7236-7244 VB denotes depicted
T18748 7244-7245 -RRB- denotes )
T18749 7247-7252 NN denotes Cells
T18750 7253-7257 VB denotes were
T18751 7258-7270 VB denotes restimulated
T18752 7271-7275 IN denotes with
T18753 7276-7279 NN denotes PMA
T18754 7280-7283 CC denotes and
T18755 7284-7293 NN denotes ionomycin
T18756 7294-7297 IN denotes for
T18757 7298-7299 CD denotes 4
T18758 7300-7302 NN denotes h.
T18759 7303-7304 -LRB- denotes (
T18760 7304-7305 NN denotes F
T18761 7305-7306 -RRB- denotes )
T18762 7307-7317 JJ denotes FACS-based
T18763 7318-7323 NN denotes assay
T18764 7324-7326 TO denotes to
T18765 7327-7334 VB denotes measure
T18766 7335-7344 JJ denotes cytolytic
T18767 7345-7353 NN denotes activity
T18768 7354-7356 IN denotes of
T18769 7357-7360 NN denotes P14
T18770 7361-7365 JJ denotes CD8+
T18771 7366-7367 NN denotes T
T18772 7368-7373 NN denotes cells
T18773 7374-7381 IN denotes against
T18774 7382-7385 NN denotes EL4
T18775 7386-7393 NN denotes targets
T18776 7394-7400 VB denotes loaded
T18777 7401-7405 IN denotes with
T18778 7406-7407 CD denotes 0
T18779 7408-7409 -LRB- denotes (
T18780 7409-7410 JJ|RB denotes −
T18781 7410-7411 -RRB- denotes )
T18782 7412-7414 CC denotes or
T18783 7415-7416 CD denotes 1
T18784 7417-7418 -LRB- denotes (
T18785 7418-7419 SYM denotes +
T18786 7419-7420 -RRB- denotes )
T18787 7421-7423 NN denotes μM
T18788 7424-7428 NN denotes Gp33
T18789 7429-7436 NN denotes peptide
T18790 7437-7438 -LRB- denotes (
T18791 7438-7456 JJ denotes effector-to-target
T18792 7457-7462 NN denotes ratio
T18793 7463-7464 SYM denotes =
T18794 7465-7466 CD denotes 5
T18795 7466-7467 SYM denotes :
T18796 7467-7468 CD denotes 1
T18797 7468-7469 -RRB- denotes )
T18798 7471-7481 NN denotes Percentage
T18799 7482-7484 IN denotes of
T18800 7485-7492 NN denotes Annexin
T18801 7493-7495 JJ denotes V+
T18802 7496-7497 -LRB- denotes (
T18803 7497-7506 JJ denotes apoptotic
T18804 7506-7507 -RRB- denotes )
T18805 7508-7514 NN denotes target
T18806 7515-7520 NN denotes cells
T18807 7521-7523 IN denotes in
T18808 7524-7527 DT denotes the
T18809 7528-7540 JJ denotes CD8-negative
T18810 7541-7544 NN denotes EL4
T18811 7545-7551 NN denotes target
T18812 7552-7562 NN denotes population
T18813 7563-7564 -LRB- denotes (
T18814 7564-7567 NN denotes dot
T18815 7568-7573 NN denotes plots
T18816 7573-7574 -RRB- denotes )
T18817 7575-7578 VB denotes was
T18818 7579-7589 VB denotes determined
T18819 7590-7591 -LRB- denotes (
T18820 7591-7601 NN denotes histograms
T18821 7601-7602 -RRB- denotes )
T18822 7604-7613 JJ denotes Cytolytic
T18823 7614-7622 NN denotes activity
T18824 7623-7626 VB denotes was
T18825 7627-7634 VB denotes blocked
T18826 7635-7637 IN denotes by
T18827 7638-7648 NN denotes incubation
T18828 7649-7653 IN denotes with
T18829 7654-7655 CD denotes 2
T18830 7656-7658 NN denotes mM
T18831 7659-7663 NN denotes EGTA
T18832 7664-7665 -LRB- denotes (
T18833 7665-7668 RB denotes not
T18834 7669-7677 VB denotes depicted
T18835 7677-7678 -RRB- denotes )
T18836 7678-7679 -COMMA- denotes ,
T18837 7680-7690 VB denotes confirming
T18838 7691-7702 NN denotes involvement
T18839 7703-7705 IN denotes of
T18840 7706-7709 DT denotes the
T18841 7710-7717 NN denotes granule
T18842 7718-7728 NN denotes exocytosis
T18843 7729-7730 -LRB- denotes (
T18844 7730-7747 NN denotes perforin–granzyme
T18845 7748-7749 NN denotes B
T18846 7749-7750 -RRB- denotes )
T18847 7751-7758 NN denotes pathway
T18848 7760-7764 NN denotes Data
T18849 7765-7768 VB denotes are
T18850 7769-7783 JJ denotes representative
T18851 7784-7786 IN denotes of
T18852 7787-7789 IN denotes at
T18853 7790-7795 JJ denotes least
T18854 7796-7800 CD denotes five
T18855 7801-7802 -LRB- denotes (
T18856 7802-7805 NN denotes A–E
T18857 7805-7806 -RRB- denotes )
T18858 7807-7809 CC denotes or
T18859 7810-7815 CD denotes three
T18860 7816-7817 -LRB- denotes (
T18861 7817-7818 NN denotes F
T18862 7818-7819 -RRB- denotes )
T18863 7820-7831 JJ denotes independent
T18864 7832-7843 NN denotes experiments
T19474 11884-11894 NN denotes Regulation
T19475 11895-11897 IN denotes of
T19476 11898-11906 NN denotes perforin
T19477 11906-11907 -COMMA- denotes ,
T19478 11908-11916 NN denotes granzyme
T19479 11917-11918 NN denotes B
T19480 11918-11919 -COMMA- denotes ,
T19481 11920-11923 CC denotes and
T19482 11924-11929 NN denotes IFN-γ
T19483 11930-11940 NN denotes expression
T19484 11941-11943 IN denotes by
T19485 11944-11949 NN denotes T-bet
T19486 11950-11953 CC denotes and
T19487 11954-11959 NN denotes Eomes
T19488 11960-11962 IN denotes in
T19489 11963-11978 VB denotes differentiating
T19490 11979-11984 NN denotes CTLs.
T19491 11985-11986 -LRB- denotes (
T19492 11986-11987 NN denotes A
T19493 11987-11988 -RRB- denotes )
T19494 11989-11994 NN denotes IFN-γ
T19495 11995-12005 NN denotes expression
T19496 12006-12008 IN denotes by
T19497 12009-12011 NN denotes WT
T19498 12012-12013 -LRB- denotes (
T19499 12013-12021 NN denotes Tbx21+/+
T19500 12021-12022 -RRB- denotes )
T19501 12023-12026 CC denotes and
T19502 12027-12042 JJ denotes T-bet–deficient
T19503 12043-12044 -LRB- denotes (
T19504 12044-12052 NN denotes Tbx21−/−
T19505 12052-12053 -RRB- denotes )
T19506 12054-12055 NN denotes T
T19507 12056-12061 NN denotes cells
T19508 12063-12068 JJ denotes Naive
T19509 12069-12073 JJ denotes CD8+
T19510 12074-12075 NN denotes T
T19511 12076-12081 NN denotes cells
T19512 12081-12082 -COMMA- denotes ,
T19513 12083-12085 CC denotes or
T19514 12086-12091 NN denotes cells
T19515 12092-12101 VB denotes activated
T19516 12102-12105 CC denotes and
T19517 12106-12114 VB denotes cultured
T19518 12115-12118 IN denotes for
T19519 12119-12120 CD denotes 4
T19520 12121-12123 CC denotes or
T19521 12124-12125 CD denotes 6
T19522 12126-12127 NN denotes d
T19523 12127-12128 -COMMA- denotes ,
T19524 12129-12133 VB denotes were
T19525 12134-12146 VB denotes restimulated
T19526 12147-12151 IN denotes with
T19527 12152-12155 NN denotes PMA
T19528 12156-12159 CC denotes and
T19529 12160-12169 NN denotes ionomycin
T19530 12170-12173 IN denotes for
T19531 12174-12175 CD denotes 6
T19532 12176-12177 NN denotes h
T19533 12177-12178 -COMMA- denotes ,
T19534 12179-12182 CC denotes and
T19535 12183-12188 NN denotes IFN-γ
T19536 12189-12199 NN denotes expression
T19537 12200-12203 VB denotes was
T19538 12204-12212 VB denotes assessed
T19539 12213-12215 IN denotes by
T19540 12216-12229 JJ denotes intracellular
T19541 12230-12238 NN denotes staining
T19542 12240-12247 NN denotes Numbers
T19543 12248-12252 VB denotes show
T19544 12253-12256 DT denotes the
T19545 12257-12267 NN denotes percentage
T19546 12268-12270 IN denotes of
T19547 12271-12277 JJ denotes IFN-γ+
T19548 12278-12284 NN denotes cells.
T19549 12285-12286 -LRB- denotes (
T19550 12286-12287 NN denotes B
T19551 12287-12288 -RRB- denotes )
T19552 12289-12297 JJ denotes Northern
T19553 12298-12302 NN denotes blot
T19554 12303-12311 NN denotes analysis
T19555 12312-12314 IN denotes of
T19556 12315-12319 NN denotes Prf1
T19557 12320-12323 CC denotes and
T19558 12324-12328 NN denotes GzmB
T19559 12329-12333 NN denotes mRNA
T19560 12334-12344 NN denotes expression
T19561 12345-12347 IN denotes in
T19562 12348-12350 NN denotes WT
T19563 12351-12353 CC denotes or
T19564 12354-12369 JJ denotes T-bet–deficient
T19565 12370-12374 JJ denotes CD8+
T19566 12375-12376 NN denotes T
T19567 12377-12382 NN denotes cells
T19568 12383-12392 VB denotes activated
T19569 12393-12396 CC denotes and
T19570 12397-12403 CC denotes either
T19571 12404-12408 JJ denotes left
T19572 12409-12419 JJ denotes uninfected
T19573 12420-12421 -LRB- denotes (
T19574 12421-12426 JJ denotes uninf
T19575 12426-12427 -RRB- denotes )
T19576 12428-12430 CC denotes or
T19577 12431-12441 VB denotes transduced
T19578 12442-12446 IN denotes with
T19579 12447-12459 NN denotes retroviruses
T19580 12460-12470 VB denotes expressing
T19581 12471-12481 NN denotes Eomes-VP16
T19582 12482-12483 -LRB- denotes (
T19583 12483-12490 NN denotes Eo-VP16
T19584 12490-12491 -RRB- denotes )
T19585 12492-12494 CC denotes or
T19586 12495-12497 DT denotes an
T19587 12498-12503 JJ denotes empty
T19588 12504-12512 NN denotes IRES-GFP
T19589 12513-12521 NN denotes cassette
T19590 12522-12523 -LRB- denotes (
T19591 12523-12526 NN denotes GFP
T19592 12526-12527 -RRB- denotes )
T19593 12529-12534 JJ denotes Total
T19594 12535-12543 JJ denotes cellular
T19595 12544-12547 NN denotes RNA
T19596 12548-12551 VB denotes was
T19597 12552-12560 VB denotes analyzed
T19598 12561-12563 IN denotes on
T19599 12564-12567 NN denotes day
T19600 12568-12569 CD denotes 6
T19601 12570-12572 IN denotes of
T19602 12573-12580 NN denotes culture
T19603 12582-12585 DT denotes The
T19604 12586-12595 NN denotes frequency
T19605 12596-12598 IN denotes of
T19606 12599-12609 VB denotes transduced
T19607 12610-12615 NN denotes cells
T19608 12616-12618 IN denotes in
T19609 12619-12622 DT denotes the
T19610 12623-12631 NN denotes cultures
T19611 12632-12635 VB denotes was
T19612 12636-12646 JJ denotes equivalent
T19613 12647-12650 IN denotes for
T19614 12651-12655 DT denotes both
T19615 12656-12666 NN denotes constructs
T19616 12667-12668 -LRB- denotes (
T19617 12668-12674 CD denotes ∼65–70
T19618 12674-12675 NN denotes %
T19619 12676-12680 NN denotes GFP+
T19620 12681-12686 NN denotes cells
T19621 12686-12687 -COLON- denotes ;
T19622 12688-12691 RB denotes not
T19623 12692-12700 VB denotes depicted
T19624 12700-12701 -RRB- denotes )
T19625 12701-12702 -LRB- denotes .
T19626 12703-12704 NN denotes (
T19627 12704-12705 -RRB- denotes C
T19628 12705-12713 NN denotes ) Granzy
T19629 12713-12714 NN denotes m
T19630 12714-12717 CC denotes e B
T19631 12718-12723 NN denotes and I
T19632 12723-12733 NN denotes FN-γ expre
T19633 12733-12735 IN denotes ss
T19634 12735-12743 NN denotes ion by T
T19635 12743-12746 CC denotes bx2
T19636 12746-12754 NN denotes 1+/+ and
T19637 12755-12756 NN denotes T
T19638 12756-12761 NN denotes bx21−
T19639 12761-12769 VB denotes /− T cel
T19640 12769-12771 IN denotes ls
T19641 12772-12784 VB denotes analyzed in
T19642 12784-12789 NN denotes resti
T19643 12789-12793 WDT denotes mula
T19644 12793-12796 VB denotes ted
T19645 12797-12801 VB denotes cell
T19646 12801-12809 VB denotes s that h
T19647 12809-12812 IN denotes ad
T19648 12812-12813 CD denotes b
T19649 12813-12815 NN denotes ee
T19650 12815-12816 -LRB- denotes n
T19651 12817-12818 NN denotes c
T19652 12818-12819 -RRB- denotes u
T19653 12819-12824 NN denotes lture
T19654 12824-12834 NN denotes d for 5 d.
T19655 12835-12837 IN denotes (D
T19656 12837-12842 NN denotes ) IFN
T19657 12842-12852 VB denotes -γ product
T19658 12852-12856 IN denotes ion
T19659 12856-12863 NN denotes by cell
T19660 12863-12865 CC denotes s
T19661 12865-12872 NN denotes transdu
T19662 12872-12873 -LRB- denotes c
T19663 12873-12876 NN denotes ed
T19664 12876-12877 -RRB- denotes w
T19665 12877-12889 NN denotes ith Eo-VP16
T19666 12889-12890 -LRB- denotes o
T19667 12890-12892 NN denotes r
T19668 12892-12893 -RRB- denotes c
T19669 12893-12901 VB denotes ontrol (
T19670 12901-12903 IN denotes GF
T19671 12903-12906 NN denotes P)
T19672 12906-12907 CD denotes r
T19673 12907-12912 IN denotes etrov
T19674 12912-12913 CD denotes i
T19675 12913-12914 NN denotes r
T19676 12914-12916 IN denotes us
T19677 12916-12929 NN denotes es (RV) measu
T19678 12929-12933 IN denotes red
T19679 12933-12936 NN denotes on
T19680 12936-12939 CC denotes day
T19681 12940-12949 NN denotes 4 after 6
T19682 12993-13000 NN denotes Numbers
T19683 13001-13005 VB denotes show
T19684 13006-13009 DT denotes the
T19685 13010-13020 NN denotes percentage
T19686 13021-13023 IN denotes of
T19687 13024-13028 JJ denotes GFP+
T19688 13029-13035 JJ denotes IFN-γ+
T19689 13036-13041 NN denotes cells
T19690 13043-13050 NN denotes Results
T19691 13051-13054 VB denotes are
T19692 13055-13069 JJ denotes representative
T19693 13070-13072 IN denotes of
T19694 13073-13078 CD denotes three
T19695 13079-13080 -LRB- denotes (
T19696 13080-13081 NN denotes A
T19697 13082-13085 CC denotes and
T19698 13086-13087 NN denotes C
T19699 13087-13088 -RRB- denotes )
T19700 13089-13091 CC denotes or
T19701 13092-13095 CD denotes two
T19702 13096-13097 -LRB- denotes (
T19703 13097-13098 NN denotes B
T19704 13099-13102 CC denotes and
T19705 13103-13104 NN denotes D
T19706 13104-13105 -RRB- denotes )
T19707 13106-13117 JJ denotes independent
T19708 13118-13129 NN denotes experiments
T21280 19206-19211 NN denotes Runx3
T21281 19212-19220 VB denotes controls
T21282 19221-19226 NN denotes Eomes
T21283 19226-19227 -COMMA- denotes ,
T21284 19228-19236 NN denotes perforin
T21285 19236-19237 -COMMA- denotes ,
T21286 19238-19246 NN denotes granzyme
T21287 19247-19248 NN denotes B
T21288 19248-19249 -COMMA- denotes ,
T21289 19250-19253 CC denotes and
T21290 19254-19259 NN denotes IFN-γ
T21291 19260-19270 NN denotes expression
T21292 19271-19273 IN denotes in
T21293 19274-19282 NN denotes effector
T21294 19283-19287 NN denotes CTLs
T21295 19289-19297 NN denotes Runx3+/+
T21296 19298-19300 CC denotes or
T21297 19301-19309 NN denotes Runx3−/−
T21298 19310-19314 JJ denotes CD8+
T21299 19315-19316 NN denotes T
T21300 19317-19322 NN denotes cells
T21301 19323-19327 VB denotes were
T21302 19328-19337 VB denotes activated
T21303 19338-19341 CC denotes and
T21304 19342-19352 VB denotes transduced
T21305 19353-19357 IN denotes with
T21306 19358-19370 NN denotes retroviruses
T21307 19371-19378 VB denotes bearing
T21308 19379-19381 DT denotes an
T21309 19382-19387 JJ denotes empty
T21310 19388-19396 NN denotes IRES-GFP
T21311 19397-19405 NN denotes cassette
T21312 19406-19407 -LRB- denotes (
T21313 19407-19410 NN denotes GFP
T21314 19410-19411 -RRB- denotes )
T21315 19412-19414 CC denotes or
T21316 19415-19419 RB denotes also
T21317 19420-19428 VB denotes encoding
T21318 19429-19439 NN denotes Eomes-VP16
T21319 19440-19441 -LRB- denotes (
T21320 19441-19448 NN denotes Eo-VP16
T21321 19448-19449 -RRB- denotes )
T21322 19450-19452 CC denotes or
T21323 19453-19462 NN denotes Myc-Runx3
T21324 19463-19464 -LRB- denotes (
T21325 19464-19469 NN denotes Runx3
T21326 19469-19470 -RRB- denotes )
T21327 19472-19475 DT denotes The
T21328 19476-19485 NN denotes frequency
T21329 19486-19488 IN denotes of
T21330 19489-19499 VB denotes transduced
T21331 19500-19505 NN denotes cells
T21332 19506-19508 IN denotes in
T21333 19509-19512 DT denotes the
T21334 19513-19521 NN denotes cultures
T21335 19522-19525 VB denotes was
T21336 19526-19536 JJ denotes equivalent
T21337 19537-19540 IN denotes for
T21338 19541-19544 DT denotes all
T21339 19545-19555 NN denotes constructs
T21340 19556-19557 -LRB- denotes (
T21341 19557-19563 CD denotes ∼75–90
T21342 19563-19564 NN denotes %
T21343 19565-19569 NN denotes GFP+
T21344 19570-19575 NN denotes cells
T21345 19575-19576 -COLON- denotes ;
T21346 19577-19580 RB denotes not
T21347 19581-19589 VB denotes depicted
T21348 19589-19590 -RRB- denotes )
T21349 19590-19591 -LRB- denotes .
T21350 19592-19593 NN denotes (
T21351 19593-19594 -RRB- denotes A
T21352 19594-19601 NN denotes ) Prote
T21353 19601-19611 NN denotes in express
T21354 19611-19613 IN denotes io
T21355 19613-19623 JJ denotes n in whole
T21356 19623-19631 NN denotes -cell ex
T21357 19631-19632 -LRB- denotes t
T21358 19632-19635 NN denotes rac
T21359 19635-19636 CD denotes t
T21360 19636-19637 -RRB- denotes s
T21361 19638-19641 VB denotes (da
T21362 19641-19649 VB denotes y 6) was
T21363 19650-19652 IN denotes an
T21364 19652-19666 NN denotes alyzed by immu
T21365 19678-19692 NN denotes Overexpression
T21366 19693-19695 IN denotes of
T21367 19696-19706 NN denotes Eomes-VP16
T21368 19707-19710 MD denotes can
T21369 19710-19713 RB denotes not
T21370 19714-19716 VB denotes be
T21371 19717-19725 VB denotes detected
T21372 19726-19730 IN denotes with
T21373 19731-19734 DT denotes the
T21374 19735-19740 NN denotes Eomes
T21375 19741-19749 NN denotes antibody
T21376 19749-19750 -COMMA- denotes ,
T21377 19751-19753 IN denotes as
T21378 19754-19757 DT denotes the
T21379 19758-19768 JJ denotes C-terminal
T21380 19769-19776 NN denotes epitope
T21381 19777-19779 VB denotes is
T21382 19780-19786 IN denotes within
T21383 19787-19790 DT denotes the
T21384 19791-19797 NN denotes region
T21385 19798-19802 WDT denotes that
T21386 19803-19806 VB denotes has
T21387 19807-19811 VB denotes been
T21388 19812-19820 VB denotes replaced
T21389 19821-19825 IN denotes with
T21390 19826-19829 DT denotes the
T21391 19830-19834 NN denotes VP16
T21392 19835-19850 NN denotes transactivation
T21393 19851-19858 NN denotes domain.
T21394 19859-19860 -LRB- denotes (
T21395 19860-19861 NN denotes B
T21396 19861-19862 -RRB- denotes )
T21397 19863-19873 NN denotes Expression
T21398 19874-19876 IN denotes of
T21399 19877-19885 NN denotes granzyme
T21400 19886-19887 NN denotes B
T21401 19888-19891 CC denotes and
T21402 19892-19897 NN denotes IFN-γ
T21403 19898-19903 IN denotes after
T21404 19904-19911 NN denotes culture
T21405 19912-19915 IN denotes for
T21406 19916-19917 CD denotes 6
T21407 19918-19919 NN denotes d
T21408 19920-19923 CC denotes and
T21409 19924-19937 NN denotes restimulation
T21410 19938-19941 IN denotes for
T21411 19942-19943 CD denotes 4
T21412 19944-19945 NN denotes h
T21413 19946-19950 IN denotes with
T21414 19951-19954 NN denotes PMA
T21415 19955-19958 CC denotes and
T21416 19959-19968 NN denotes ionomycin
T21417 19969-19972 VB denotes was
T21418 19973-19983 VB denotes determined
T21419 19984-19986 IN denotes by
T21420 19987-20000 JJ denotes intracellular
T21421 20001-20009 NN denotes staining
T21422 20011-20014 DT denotes The
T21423 20015-20025 NN denotes percentage
T21424 20026-20028 IN denotes of
T21425 20029-20039 RB denotes positively
T21426 20040-20047 VB denotes stained
T21427 20048-20053 NN denotes cells
T21428 20054-20056 VB denotes is
T21429 20057-20062 VB denotes shown
T21430 20063-20068 IN denotes above
T21431 20069-20072 DT denotes the
T21432 20073-20077 NN denotes gate
T21433 20077-20078 -COLON- denotes ;
T21434 20079-20082 DT denotes the
T21435 20083-20087 JJ denotes mean
T21436 20088-20100 NN denotes fluorescence
T21437 20101-20110 NN denotes intensity
T21438 20111-20112 -LRB- denotes (
T21439 20112-20115 NN denotes MFI
T21440 20115-20116 -RRB- denotes )
T21441 20117-20119 IN denotes of
T21442 20120-20128 NN denotes granzyme
T21443 20129-20130 NN denotes B
T21444 20131-20139 NN denotes staining
T21445 20140-20143 IN denotes for
T21446 20144-20147 DT denotes the
T21447 20148-20153 JJ denotes total
T21448 20154-20164 NN denotes population
T21449 20165-20167 VB denotes is
T21450 20168-20173 VB denotes shown
T21451 20174-20179 IN denotes below
T21452 20180-20183 DT denotes the
T21453 20184-20188 NN denotes gate
T21454 20190-20193 DT denotes The
T21455 20194-20202 JJ denotes vertical
T21456 20203-20207 JJ denotes gray
T21457 20208-20213 NN denotes lines
T21458 20214-20222 VB denotes indicate
T21459 20223-20226 DT denotes the
T21460 20227-20230 NN denotes MFI
T21461 20231-20234 IN denotes for
T21462 20235-20237 JJ denotes WT
T21463 20238-20242 NN denotes GFP+
T21464 20243-20248 NN denotes cells
T21465 20250-20257 NN denotes Results
T21466 20258-20261 VB denotes are
T21467 20262-20276 JJ denotes representative
T21468 20277-20279 IN denotes of
T21469 20280-20282 IN denotes at
T21470 20283-20288 JJ denotes least
T21471 20289-20292 CD denotes two
T21472 20293-20304 JJ denotes independent
T21473 20305-20317 NN denotes experiments.
T21474 20318-20319 -LRB- denotes (
T21475 20319-20320 NN denotes C
T21476 20320-20321 -RRB- denotes )
T21477 20322-20331 JJ denotes Schematic
T21478 20332-20339 NN denotes diagram
T21479 20340-20342 IN denotes of
T21480 20343-20346 DT denotes the
T21481 20347-20362 JJ denotes transcriptional
T21482 20363-20370 NN denotes network
T21483 20371-20380 VB denotes involving
T21484 20381-20386 NN denotes Runx3
T21485 20387-20390 CC denotes and
T21486 20391-20396 NN denotes T-box
T21487 20397-20404 NN denotes factors
T21488 20406-20411 NN denotes T-bet
T21489 20412-20414 VB denotes is
T21490 20415-20422 VB denotes induced
T21491 20423-20425 IN denotes by
T21492 20426-20429 NN denotes TCR
T21493 20430-20437 NN denotes signals
T21494 20438-20441 CC denotes and
T21495 20442-20444 VB denotes is
T21496 20445-20454 JJ denotes essential
T21497 20455-20458 IN denotes for
T21498 20459-20464 JJ denotes early
T21499 20465-20470 NN denotes IFN-γ
T21500 20471-20481 NN denotes expression
T21501 20483-20488 NN denotes Runx3
T21502 20489-20491 VB denotes is
T21503 20492-20499 JJ denotes present
T21504 20500-20502 IN denotes in
T21505 20503-20508 JJ denotes naive
T21506 20509-20513 JJ denotes CD8+
T21507 20514-20515 NN denotes T
T21508 20516-20521 NN denotes cells
T21509 20522-20525 CC denotes and
T21510 20526-20535 VB denotes represses
T21511 20536-20541 NN denotes Runx1
T21512 20542-20545 CC denotes and
T21513 20546-20553 VB denotes induces
T21514 20554-20559 NN denotes Eomes
T21515 20559-20560 -COMMA- denotes ,
T21516 20561-20569 NN denotes perforin
T21517 20569-20570 -COMMA- denotes ,
T21518 20571-20579 NN denotes granzyme
T21519 20580-20581 NN denotes B
T21520 20581-20582 -COMMA- denotes ,
T21521 20583-20586 CC denotes and
T21522 20587-20592 NN denotes IFN-γ
T21523 20593-20603 NN denotes expression
T21524 20605-20610 NN denotes Eomes
T21525 20611-20614 MD denotes may
T21526 20615-20626 VB denotes participate
T21527 20627-20629 IN denotes in
T21528 20630-20640 VB denotes sustaining
T21529 20641-20645 JJ denotes late
T21530 20646-20651 NN denotes IFN-γ
T21531 20652-20662 NN denotes expression
T21532 20662-20663 -COMMA- denotes ,
T21533 20664-20671 IN denotes whereas
T21534 20672-20677 NN denotes Runx3
T21535 20678-20681 CC denotes and
T21536 20682-20687 NN denotes Eomes
T21537 20688-20689 -LRB- denotes (
T21538 20689-20692 CC denotes but
T21539 20693-20696 RB denotes not
T21540 20697-20702 NN denotes T-bet
T21541 20702-20703 -RRB- denotes )
T21542 20704-20707 MD denotes may
T21543 20708-20717 VB denotes cooperate
T21544 20718-20720 TO denotes to
T21545 20721-20729 VB denotes activate
T21546 20730-20738 NN denotes perforin
T21547 20739-20749 NN denotes expression
T21548 20751-20754 DT denotes The
T21549 20755-20761 JJ denotes dotted
T21550 20762-20766 NN denotes line
T21551 20767-20776 VB denotes indicates
T21552 20777-20780 DT denotes the
T21553 20781-20788 JJ denotes partial
T21554 20789-20795 NN denotes effect
T21555 20796-20798 IN denotes of
T21556 20799-20804 JJ denotes T-bet
T21557 20805-20815 NN denotes deficiency
T21558 20816-20818 IN denotes on
T21559 20819-20823 NN denotes Gzmb
T21560 20824-20828 NN denotes mRNA
T21561 20829-20832 CC denotes but
T21562 20833-20836 RB denotes not
T21563 20837-20845 NN denotes granzyme
T21564 20846-20847 NN denotes B
T21565 20848-20855 NN denotes protein
T21566 20856-20866 NN denotes expression
T17829 33613-33621 JJ denotes Northern
T17830 33622-33625 CC denotes and
T17831 33626-33633 JJ denotes Western
T17832 33634-33638 NN denotes blot
T17833 33639-33647 NN denotes analyses
T17834 33649-33652 NN denotes RNA
T17835 33653-33662 NN denotes isolation
T17836 33663-33666 CC denotes and
T17837 33667-33675 JJ denotes Northern
T17838 33676-33680 NN denotes blot
T17839 33681-33689 NN denotes analysis
T17840 33690-33693 VB denotes was
T17841 33694-33703 VB denotes performed
T17842 33704-33706 IN denotes as
T17843 33707-33717 RB denotes previously
T17844 33718-33727 VB denotes described
T17845 33728-33729 -LRB- denotes (
T17846 33729-33731 CD denotes 29
T17847 33731-33732 -RRB- denotes )
T17848 33734-33736 IN denotes In
T17849 33737-33742 NN denotes brief
T16598 30165-30175 RB denotes previously
T16599 30176-30185 VB denotes optimized
T16600 30186-30200 NN denotes concentrations
T16601 30200-30201 -COMMA- denotes ,
T16602 30202-30211 VB denotes incubated
T16603 30212-30215 IN denotes for
T16604 30216-30218 CD denotes 15
T16605 30219-30222 NN denotes min
T16606 30223-30225 IN denotes at
T16607 30226-30230 NN denotes room
T16608 30231-30242 NN denotes temperature
R201 T270 T271 arg1Of Runx3,and
R202 T272 T271 arg2Of T-box,and
R203 T273 T270 arg1Of proteins,Runx3
R204 T273 T272 arg1Of proteins,T-box
R205 T273 T274 arg1Of proteins,cooperate
R206 T273 T276 arg1Of proteins,establish
R207 T276 T274 arg2Of establish,cooperate
R208 T276 T275 arg1Of establish,to
R209 T279 T276 arg2Of program,establish
R210 T279 T277 arg1Of program,the
R211 T279 T278 arg1Of program,transcriptional
R212 T279 T280 arg1Of program,of
R213 T282 T280 arg2Of CTLs,of
R214 T282 T281 arg1Of CTLs,effector
R215 T283 T284 arg1Of Activation,of
R216 T283 T289 arg1Of Activation,with
R217 T283 T291 arg1Of Activation,induces
R218 T288 T284 arg2Of cells,of
R219 T288 T285 arg1Of cells,naive
R220 T288 T286 arg1Of cells,CD8+
R221 T288 T287 arg1Of cells,T
R222 T290 T289 arg2Of antigen,with
R223 T293 T291 arg2Of differentiation,induces
R224 T293 T292 arg1Of differentiation,their
R225 T293 T294 arg1Of differentiation,into
R226 T298 T294 arg2Of lymphocytes,into
R227 T298 T295 arg1Of lymphocytes,effector
R228 T298 T296 arg1Of lymphocytes,cytolytic
R229 T298 T297 arg1Of lymphocytes,T
R230 T298 T299 arg1Of lymphocytes,(
R231 T300 T299 arg2Of CTLs,(
R232 T301 T299 arg3Of ),(
R233 T302 T303 arg1Of CTLs,lyse
R234 T303 T309 arg1Of lyse,by
R235 T304 T305 arg1Of infected,or
R236 T306 T305 arg2Of aberrant,or
R237 T308 T303 arg2Of cells,lyse
R238 T308 T304 arg1Of cells,infected
R239 T308 T306 arg1Of cells,aberrant
R240 T308 T307 arg1Of cells,target
R241 T310 T309 arg2Of exocytosis,by
R242 T310 T311 arg1Of exocytosis,of
R243 T313 T311 arg2Of granules,of
R244 T313 T312 arg1Of granules,lytic
R245 T313 T314 arg1Of granules,containing
R246 T318 T315 arg1Of perforin,the
R247 T318 T316 arg1Of perforin,pore-forming
R248 T318 T317 arg1Of perforin,protein
R249 T318 T319 arg1Of perforin,and
R250 T319 T314 arg2Of and,containing
R251 T321 T319 arg2Of family,and
R252 T321 T320 arg1Of family,a
R253 T321 T322 arg1Of family,of
R254 T323 T322 arg2Of proteases,of
R255 T323 T324 arg2Of proteases,termed
R256 T325 T324 arg3Of granzymes,termed
R257 T326 T327 arg1Of We,show
R258 T331 T329 arg1Of differentiation,effector
R259 T331 T330 arg1Of differentiation,CTL
R260 T331 T332 arg1Of differentiation,occurs
R261 T332 T327 arg2Of occurs,show
R262 T332 T328 arg1Of occurs,that
R263 T332 T333 arg1Of occurs,in
R264 T336 T334 arg1Of phases,two
R265 T336 T335 arg1Of phases,sequential
R266 T336 T338 arg1Of phases,vitro
R267 T336 T339 arg1Of phases,","
R268 T336 T340 arg2Of phases,characterized
R269 T336 T354 arg1Of phases,","
R270 T338 T337 arg1Of vitro,in
R271 T343 T340 arg1Of induction,characterized
R272 T343 T341 arg2Of induction,by
R273 T343 T342 arg1Of induction,early
R274 T343 T344 arg1Of induction,of
R275 T345 T346 arg1Of T-bet,and
R276 T347 T346 arg2Of late,and
R277 T348 T344 arg2Of induction,of
R278 T348 T345 arg1Of induction,T-bet
R279 T348 T347 arg1Of induction,late
R280 T348 T349 arg1Of induction,of
R281 T350 T349 arg2Of Eomesodermin,of
R282 T350 T351 arg1Of Eomesodermin,(
R283 T352 T351 arg2Of Eomes,(
R284 T353 T351 arg3Of ),(
R285 T354 T333 arg2Of ",",in
R286 T354 T358 arg1Of ",",that
R287 T354 T359 arg1Of ",",regulate
R288 T357 T354 arg2Of factors,","
R289 T357 T355 arg1Of factors,T-box
R290 T357 T356 arg1Of factors,transcription
R291 T359 T372 arg1Of regulate,","
R292 T359 T373 arg1Of regulate,respectively
R293 T361 T362 arg1Of early,and
R294 T363 T362 arg2Of late,and
R295 T364 T359 arg2Of phases,regulate
R296 T364 T360 arg1Of phases,the
R297 T364 T361 arg1Of phases,early
R298 T364 T363 arg1Of phases,late
R299 T364 T365 arg1Of phases,of
R300 T368 T367 arg2Of IFN,(
R301 T369 T367 arg3Of ),(
R302 T371 T365 arg2Of expression,of
R303 T371 T366 arg1Of expression,interferon
R304 T371 T367 arg1Of expression,(
R305 T371 T370 arg1Of expression,γ
R306 T375 T374 arg2Of addition,In
R307 T377 T378 arg1Of we,demonstrate
R308 T378 T374 arg1Of demonstrate,In
R309 T378 T376 arg1Of demonstrate,","
R310 T381 T378 arg2Of role,demonstrate
R311 T381 T379 arg1Of role,a
R312 T381 T380 arg1Of role,critical
R313 T381 T382 arg1Of role,for
R314 T381 T387 arg1Of role,in
R315 T386 T382 arg2Of Runx3,for
R316 T386 T383 arg1Of Runx3,the
R317 T386 T384 arg1Of Runx3,transcription
R318 T386 T385 arg1Of Runx3,factor
R319 T389 T387 arg2Of differentiation,in
R320 T389 T388 arg1Of differentiation,CTL
R321 T390 T391 arg1Of Runx3,regulates
R322 T393 T396 arg1Of expression,as
R323 T396 T391 arg2Of as,regulates
R324 T396 T392 arg1Of as,Eomes
R325 T396 T394 arg1Of as,as
R326 T396 T395 arg1Of as,well
R327 T397 T396 arg2Of expression,as
R328 T397 T398 arg1Of expression,of
R329 T401 T398 arg2Of markers,of
R330 T401 T399 arg1Of markers,three
R331 T401 T400 arg1Of markers,cardinal
R332 T401 T402 arg1Of markers,of
R333 T401 T407 arg1Of markers,:
R334 T406 T402 arg2Of program,of
R335 T406 T403 arg1Of program,the
R336 T406 T404 arg1Of program,effector
R337 T406 T405 arg1Of program,CTL
R338 T408 T409 arg1Of IFN-γ,","
R339 T409 T412 arg1Of ",",and
R340 T410 T409 arg2Of perforin,","
R341 T412 T407 arg2Of and,:
R342 T412 T411 arg1Of and,","
R343 T414 T412 arg2Of B.,and
R344 T414 T413 arg1Of B.,granzyme
R345 T416 T415 arg1Of data,Our
R346 T416 T417 arg1Of data,point
R347 T417 T391 arg3Of point,regulates
R348 T417 T418 arg1Of point,to
R349 T420 T418 arg2Of existence,to
R350 T420 T419 arg1Of existence,the
R351 T420 T421 arg1Of existence,of
R352 T420 T426 arg2Of existence,in
R353 T420 T427 arg1Of existence,which
R354 T425 T421 arg2Of network,of
R355 T425 T422 arg1Of network,an
R356 T425 T423 arg1Of network,elaborate
R357 T425 T424 arg1Of network,transcriptional
R358 T428 T430 arg1Of Runx3,induces
R359 T428 T433 arg1Of Runx3,cooperates
R360 T428 T439 arg1Of Runx3,regulate
R361 T430 T431 arg1Of induces,and
R362 T431 T426 arg1Of and,in
R363 T431 T429 arg1Of and,initially
R364 T433 T431 arg2Of cooperates,and
R365 T433 T432 arg1Of cooperates,then
R366 T433 T434 arg1Of cooperates,with
R367 T433 T438 modOf cooperates,to
R368 T437 T434 arg2Of factors,with
R369 T437 T435 arg1Of factors,T-box
R370 T437 T436 arg1Of factors,transcription
R371 T439 T438 arg1Of regulate,to
R372 T441 T439 arg2Of transcription,regulate
R373 T441 T440 arg1Of transcription,gene
R374 T441 T442 arg1Of transcription,in
R375 T444 T442 arg2Of CTLs,in
R376 T444 T443 arg1Of CTLs,differentiating
R1230 T1505 T1503 arg1Of cells,Naive
R1231 T1505 T1504 arg1Of cells,CD8+ T
R1232 T1505 T1506 arg1Of cells,differentiate
R1233 T1506 T1507 arg1Of differentiate,into
R1234 T1506 T1510 arg1Of differentiate,with
R1235 T1509 T1507 arg2Of CTLs,into
R1236 T1509 T1508 arg1Of CTLs,effector
R1237 T1512 T1510 arg2Of ability,with
R1238 T1512 T1511 arg1Of ability,the
R1239 T1512 T1513 modOf ability,to
R1240 T1512 T1529 modOf ability,to
R1241 T1514 T1513 arg1Of lyse,to
R1242 T1514 T1518 arg1Of lyse,by
R1243 T1514 T1528 arg1Of lyse,and
R1244 T1517 T1514 arg2Of cells,lyse
R1245 T1517 T1515 arg1Of cells,antigen-bearing
R1246 T1517 T1516 arg1Of cells,target
R1247 T1519 T1518 arg2Of exocytosis,by
R1248 T1519 T1520 arg1Of exocytosis,of
R1249 T1522 T1520 arg2Of granules,of
R1250 T1522 T1521 arg1Of granules,lytic
R1251 T1522 T1523 arg1Of granules,containing
R1252 T1524 T1525 arg1Of perforin,and
R1253 T1525 T1523 arg2Of and,containing
R1254 T1526 T1525 arg2Of granzymes,and
R1255 T1528 T1527 arg1Of and,","
R1256 T1530 T1528 arg2Of produce,and
R1257 T1530 T1529 arg1Of produce,to
R1258 T1532 T1530 arg2Of cytokines,produce
R1259 T1532 T1531 arg1Of cytokines,inflammatory
R1260 T1532 T1534 arg1Of cytokines,as
R1261 T1534 T1533 arg1Of as,such
R1262 T1535 T1536 arg1Of IFN-γ,and
R1263 T1536 T1534 arg2Of and,as
R1264 T1536 T1538 arg1Of and,upon
R1265 T1536 T1540 arg1Of and,through
R1266 T1537 T1536 arg2Of TNF,and
R1267 T1539 T1538 arg2Of restimulation,upon
R1268 T1542 T1540 arg2Of TCR,through
R1269 T1542 T1541 arg1Of TCR,the
R1270 T1542 T1543 arg1Of TCR,(
R1271 T1544 T1543 arg2Of "1, 2",(
R1272 T1545 T1543 arg3Of ),(
R1273 T1547 T1546 arg1Of vivo,In
R1274 T1548 T1547 arg1Of experiments,vivo
R1275 T1548 T1549 arg1Of experiments,have
R1276 T1548 T1550 arg1Of experiments,elucidated
R1277 T1550 T1549 arg2Of elucidated,have
R1278 T1553 T1550 arg2Of parameters,elucidated
R1279 T1553 T1551 arg1Of parameters,many
R1280 T1553 T1552 arg1Of parameters,critical
R1281 T1553 T1554 arg1Of parameters,governing
R1282 T1556 T1557 arg1Of development,and
R1283 T1557 T1554 arg2Of and,governing
R1284 T1557 T1555 arg1Of and,the
R1285 T1557 T1559 arg1Of and,of
R1286 T1558 T1557 arg2Of evolution,and
R1287 T1562 T1559 arg2Of responses,of
R1288 T1562 T1560 arg1Of responses,primary
R1289 T1562 T1561 arg1Of responses,CTL
R1290 T1562 T1563 arg1Of responses,(
R1291 T1564 T1563 arg2Of "3, 4",(
R1292 T1565 T1563 arg3Of ),(
R1293 T1568 T1566 arg2Of study,In
R1294 T1568 T1567 arg1Of study,this
R1295 T1570 T1571 arg1Of we,have
R1296 T1570 T1572 arg1Of we,used
R1297 T1570 T1586 arg1Of we,define
R1298 T1572 T1571 arg2Of used,have
R1299 T1572 T1605 arg1Of used,and
R1300 T1574 T1573 arg1Of vitro,in
R1301 T1575 T1572 arg2Of systems,used
R1302 T1575 T1574 arg1Of systems,vitro
R1303 T1575 T1577 arg1Of systems,as
R1304 T1575 T1579 arg2Of systems,developed
R1305 T1577 T1576 arg1Of as,such
R1306 T1578 T1577 arg2Of those,as
R1307 T1579 T1580 modOf developed,to
R1308 T1581 T1580 arg1Of study,to
R1309 T1584 T1581 arg2Of differentiation,study
R1310 T1584 T1582 arg1Of differentiation,CD4+ T
R1311 T1584 T1583 arg1Of differentiation,cell
R1312 T1586 T1572 arg3Of define,used
R1313 T1586 T1585 arg1Of define,to
R1314 T1589 T1586 arg2Of basis,define
R1315 T1589 T1587 arg1Of basis,the
R1316 T1589 T1588 arg1Of basis,molecular
R1317 T1589 T1590 arg1Of basis,of
R1318 T1593 T1591 arg1Of differentiation,effector
R1319 T1593 T1592 arg1Of differentiation,CTL
R1320 T1593 T1594 arg1Of differentiation,(
R1321 T1595 T1594 arg2Of "5, 6",(
R1322 T1596 T1594 arg3Of ),(
R1323 T1601 T1590 arg2Of Eomesodermin,of
R1324 T1604 T1602 arg3Of ),(
R1325 T1601 T1593 arg1Of Eomesodermin,differentiation
R1326 T1601 T1597 arg1Of Eomesodermin,". The"
R1327 T1601 T1598 arg1Of Eomesodermin,T-box
R1328 T1605 T1566 arg1Of and,In
R1329 T1605 T1569 arg1Of and,","
R1330 T1601 T1599 arg1Of Eomesodermin,transcription
R1331 T1601 T1600 arg1Of Eomesodermin,factors
R1332 T1606 T1607 arg1Of T-bet,are
R1333 T1606 T1608 arg2Of T-bet,needed
R1334 T1601 T1602 arg1Of Eomesodermin,(
R1335 T1603 T1602 arg2Of Eomes,(
R1336 T1608 T1605 arg2Of needed,and
R1337 T1608 T1607 arg2Of needed,are
R1338 T1608 T1609 arg1Of needed,for
R1339 T1611 T1610 arg1Of aspects,important
R1340 T1611 T1612 arg1Of aspects,of
R1341 T1611 T1614 arg1Of aspects,and
R1342 T1613 T1612 arg2Of effector,of
R1343 T1705 T1686 arg1Of resolved,Nevertheless
R1344 T1614 T1609 arg2Of and,for
R1345 T1617 T1614 arg2Of differentiation,and
R1346 T1617 T1615 arg1Of differentiation,memory
R1347 T1617 T1616 arg1Of differentiation,CTL
R1348 T1617 T1618 arg1Of differentiation,(
R1349 T1619 T1618 arg2Of 7,(
R1350 T1620 T1618 arg3Of ),(
R1351 T1623 T1621 arg2Of mice,In
R1352 T1623 T1622 arg1Of mice,uninfected
R1353 T1705 T1687 arg1Of resolved,","
R1354 T1705 T1701 arg2Of resolved,have
R1355 T1626 T1625 arg1Of deletion,compound
R1356 T1626 T1627 arg1Of deletion,of
R1357 T1626 T1634 arg1Of deletion,is
R1358 T1626 T1635 arg2Of deletion,associated
R1359 T1705 T1702 arg1Of resolved,not
R1360 T1705 T1703 arg1Of resolved,yet
R1361 T1705 T1704 arg2Of resolved,been
R1362 T1705 T1706 arg1Of resolved,:
R1363 T1631 T1629 arg2Of T-bet,(
R1364 T1631 T1630 arg1Of T-bet,encoding
R1365 T1632 T1629 arg3Of ),(
R1366 T1708 T1707 arg2Of particular,in
R1367 T1633 T1627 arg2Of and eomesodermin genes,of
R1368 T1633 T1628 arg1Of and eomesodermin genes,the Tbx21
R1369 T1633 T1629 arg1Of and eomesodermin genes,(
R1370 T1710 T1711 arg1Of it,is
R1371 T1635 T1621 arg1Of associated,In
R1372 T1635 T1624 arg1Of associated,","
R1373 T1635 T1634 arg2Of associated,is
R1374 T1635 T1636 arg1Of associated,with
R1375 T1639 T1636 arg2Of loss,with
R1376 T1710 T1713 arg2Of it,known
R1377 T1713 T1706 arg2Of known,:
R1378 T1639 T1637 arg1Of loss,a
R1379 T1639 T1638 arg1Of loss,selective
R1380 T1639 T1640 arg1Of loss,of
R1381 T1639 T1643 arg1Of loss,with
R1382 T1642 T1640 arg2Of cells,of
R1383 T1642 T1641 arg1Of cells,CD8+ T
R1384 T1713 T1707 arg1Of known,in
R1385 T1713 T1709 arg1Of known,","
R1386 T1645 T1643 arg2Of IL-2Rβ–high,with
R1387 T1645 T1644 arg1Of IL-2Rβ–high,an
R1388 T1645 T1646 arg1Of IL-2Rβ–high,","
R1389 T1713 T1711 arg2Of known,is
R1390 T1713 T1712 arg1Of known,not
R1391 T1717 T1715 arg1Of factors,these
R1392 T1648 T1646 arg2Of phenotype,","
R1393 T1648 T1647 arg1Of phenotype,memory
R1394 T1648 T1649 arg1Of phenotype,(
R1395 T1650 T1649 arg2Of 8,(
R1396 T1651 T1649 arg3Of ),(
R1397 T1652 T1653 arg1Of Mice,deficient
R1398 T1652 T1662 arg1Of Mice,have
R1399 T1652 T1663 arg1Of Mice,impaired
R1400 T1652 T1669 arg1Of Mice,manifest
R1401 T1652 T1675 arg1Of Mice,fail
R1402 T1652 T1677 arg1Of Mice,control
R1403 T1653 T1654 arg1Of deficient,for
R1404 T1656 T1657 arg1Of T-bet,and
R1405 T1657 T1654 arg2Of and,for
R1406 T1657 T1655 arg1Of and,both
R1407 T1657 T1659 arg1Of and,in
R1408 T1658 T1657 arg2Of Eomes,and
R1409 T1717 T1716 arg1Of factors,transcription
R1410 T1717 T1718 arg1Of factors,function
R1411 T1661 T1659 arg2Of cells,in
R1412 T1661 T1660 arg1Of cells,T
R1413 T1717 T1721 arg1Of factors,control
R1414 T1718 T1714 arg1Of function,whether
R1415 T1663 T1662 arg2Of impaired,have
R1416 T1663 T1668 arg1Of impaired,","
R1417 T1664 T1663 arg2Of expression,impaired
R1418 T1664 T1665 arg1Of expression,of
R1419 T1718 T1719 arg1Of function,redundantly
R1420 T1667 T1665 arg2Of mediators,of
R1421 T1667 T1666 arg1Of mediators,cytolytic
R1422 T1718 T1720 modOf function,to
R1423 T1668 T1674 arg1Of ",",and
R1424 T1718 T1727 arg1Of function,and
R1425 T1669 T1668 arg2Of manifest,","
R1426 T1721 T1720 arg1Of control,to
R1427 T1672 T1669 arg2Of activity,manifest
R1428 T1672 T1670 arg1Of activity,poor
R1429 T1672 T1671 arg1Of activity,cytolytic
R1430 T1674 T1673 arg1Of and,","
R1431 T1675 T1674 arg2Of fail,and
R1432 T1725 T1721 arg2Of differentiation,control
R1433 T1677 T1675 arg2Of control,fail
R1434 T1677 T1676 arg1Of control,to
R1435 T1682 T1677 arg2Of infection,control
R1436 T1682 T1678 arg1Of infection,acute
R1437 T1682 T1679 arg1Of infection,lymphocytic
R1438 T1682 T1680 arg1Of infection,choriomeningitis
R1439 T1682 T1681 arg1Of infection,virus
R1440 T1682 T1683 arg1Of infection,(
R1441 T1684 T1683 arg2Of 9,(
R1442 T1725 T1722 arg1Of differentiation,effector
R1443 T1685 T1683 arg3Of ),(
R1444 T1725 T1723 arg1Of differentiation,CD8+ T
R1445 T1725 T1724 arg1Of differentiation,cell
R1446 T1727 T1713 arg3Of and,known
R1447 T1690 T1688 arg1Of roles,the
R1448 T1690 T1689 arg1Of roles,specific
R1449 T1690 T1691 arg1Of roles,of
R1450 T1690 T1695 arg1Of roles,in
R1451 T1690 T1701 arg1Of roles,have
R1452 T1690 T1704 arg1Of roles,been
R1453 T1690 T1705 arg2Of roles,resolved
R1454 T1692 T1693 arg1Of T-bet,and
R1455 T1693 T1691 arg2Of and,of
R1456 T1694 T1693 arg2Of Eomes,and
R1457 T1727 T1726 arg1Of and,","
R1458 T1697 T1696 arg1Of expansion,clonal
R1459 T1697 T1698 arg1Of expansion,and
R1460 T1728 T1727 arg2Of whether,and
R1461 T1698 T1695 arg2Of and,in
R1462 T1700 T1698 arg2Of differentiation,and
R1463 T1729 T1730 arg1Of they,do
R1464 T1700 T1699 arg1Of differentiation,CTL
R1465 T1730 T1728 arg2Of do,whether
R1466 T1730 T1731 arg1Of do,so
R1467 T1730 T1732 arg1Of do,directly
R1468 T1730 T1733 arg1Of do,by
R1469 T1734 T1733 arg2Of targeting,by
R1470 T1737 T1735 arg1Of cytokine,specific
R1471 T1799 T1798 arg1Of expression,its
R1472 T1799 T1800 arg1Of expression,is
R1473 T1799 T1801 arg2Of expression,up-regulated
R1474 T1801 T1800 arg2Of up-regulated,is
R1475 T1801 T1802 arg1Of up-regulated,during
R1476 T1801 T1807 arg1Of up-regulated,and
R1477 T1737 T1736 arg1Of cytokine,effector
R1478 T1737 T1738 arg1Of cytokine,and
R1479 T1804 T1803 arg1Of cell,Th1
R1480 T1738 T1734 arg2Of and,targeting
R1481 T1805 T1802 arg2Of differentiation,during
R1482 T1805 T1804 arg1Of differentiation,cell
R1483 T1738 T1742 arg1Of and,","
R1484 T1807 T1788 arg1Of and,Although
R1485 T1807 T1797 arg1Of and,","
R1486 T1807 T1806 arg1Of and,","
R1487 T1808 T1809 arg1Of Runx3,influences
R1488 T1738 T1750 arg1Of and,","
R1489 T1809 T1807 arg2Of influences,and
R1490 T1809 T1815 arg1Of influences,through
R1491 T1741 T1738 arg2Of proteins,and
R1492 T1812 T1813 arg1Of differentiation,and
R1493 T1813 T1809 arg2Of and,influences
R1494 T1813 T1810 arg1Of and,Th1
R1495 T1813 T1811 arg1Of and,cell
R1496 T1814 T1813 arg2Of function,and
R1497 T1741 T1739 arg1Of proteins,cytolytic
R1498 T1817 T1815 arg2Of regulation,through
R1499 T1817 T1816 arg1Of regulation,direct
R1500 T1817 T1818 arg1Of regulation,of
R1501 T1820 T1818 arg2Of genes,of
R1502 T1820 T1819 arg1Of genes,the Il4 and Ifng cytokine
R1503 T1820 T1821 arg1Of genes,(
R1504 T1741 T1740 arg1Of proteins,"genes. Runx"
R1505 T1744 T1742 arg2Of family,","
R1506 T1822 T1821 arg2Of "15, 16",(
R1507 T1823 T1821 arg3Of ),(
R1508 T1825 T1824 arg2Of contrast,In
R1509 T1744 T1743 arg1Of family,a
R1510 T1744 T1745 arg1Of family,of
R1511 T1749 T1745 arg2Of factors,of
R1512 T1830 T1827 arg1Of proteins,all
R1513 T1830 T1828 arg1Of proteins,three
R1514 T1830 T1829 arg1Of proteins,Runx
R1515 T1830 T1831 arg1Of proteins,are
R1516 T1830 T1832 arg2Of proteins,expressed
R1517 T1832 T1824 arg1Of expressed,In
R1518 T1832 T1826 arg1Of expressed,","
R1519 T1832 T1831 arg2Of expressed,are
R1520 T1832 T1833 arg1Of expressed,in
R1521 T1832 T1841 arg1Of expressed,and
R1522 T1836 T1833 arg2Of cells,in
R1523 T1836 T1834 arg1Of cells,mature
R1524 T1836 T1835 arg1Of cells,CD8+ T
R1525 T1836 T1837 arg1Of cells,(
R1526 T1838 T1837 arg2Of "10, 12",(
R1527 T1749 T1746 arg1Of factors,three
R1528 T1839 T1837 arg3Of ),(
R1529 T1749 T1747 arg1Of factors,DNA-binding
R1530 T1749 T1748 arg1Of factors,transcription
R1531 T1841 T1840 arg1Of and,","
R1532 T1753 T1751 arg1Of differentiation,control
R1533 T1844 T1842 arg1Of cells,Runx3-deficient
R1534 T1844 T1843 arg1Of cells,CD8+ T
R1535 T1844 T1845 arg1Of cells,show
R1536 T1845 T1841 arg2Of show,and
R1537 T1753 T1752 arg1Of differentiation,thymocyte
R1538 T1848 T1845 arg2Of activity,show
R1539 T1848 T1846 arg2Of activity,reduced
R1540 T1848 T1847 arg1Of activity,cytolytic
R1541 T1848 T1849 arg1Of activity,(
R1542 T1753 T1754 arg1Of differentiation,and
R1543 T1850 T1849 arg2Of "12, 13",(
R1544 T1754 T1750 arg2Of and,","
R1545 T1851 T1849 arg3Of ),(
R1546 T1852 T1854 arg1Of We,tested
R1547 T1758 T1754 arg2Of decision,and
R1548 T1854 T1853 arg1Of tested,therefore
R1549 T1856 T1857 arg1Of Runx3,influenced
R1550 T1857 T1854 arg2Of influenced,tested
R1551 T1857 T1855 arg1Of influenced,whether
R1552 T1857 T1863 arg1Of influenced,report
R1553 T1758 T1755 arg1Of decision,the
R1554 T1758 T1756 arg1Of decision,CD4/CD8
R1555 T1758 T1757 arg1Of decision,lineage
R1556 T1861 T1857 arg2Of "differentiation. In",influenced
R1557 T1861 T1858 arg1Of "differentiation. In",cytolytic
R1558 T1861 T1859 arg1Of "differentiation. In",T
R1559 T1861 T1860 arg1Of "differentiation. In",cell
R1560 T1758 T1759 arg1Of decision,(
R1561 T1863 T1862 arg1Of report,this
R1562 T1863 T1866 modOf report,show
R1563 T1760 T1759 arg2Of 10–13,(
R1564 T1865 T1866 arg1Of we,show
R1565 T1866 T1864 arg1Of show,","
R1566 T1761 T1759 arg3Of ),(
R1567 T1868 T1869 arg1Of Runx3,and
R1568 T1870 T1869 arg2Of T-box,and
R1569 T1762 T1763 arg1Of Runx3,and
R1570 T1871 T1868 arg1Of factors,Runx3
R1571 T1871 T1870 arg1Of factors,T-box
R1572 T1871 T1873 arg1Of factors,regulate
R1573 T1873 T1866 arg2Of regulate,show
R1574 T1873 T1867 arg1Of regulate,that
R1575 T1873 T1872 arg1Of regulate,synergistically
R1576 T1763 T1766 arg1Of and,are
R1577 T1875 T1876 arg1Of differentiation,and
R1578 T1876 T1873 arg2Of and,regulate
R1579 T1876 T1874 arg1Of and,CTL
R1580 T1763 T1767 arg2Of and,expressed
R1581 T1877 T1876 arg2Of function,and
R1582 T1765 T1763 arg2Of mRNA,and
R1583 T1878 T1879 arg1Of T-bet,is
R1584 T1878 T1880 arg2Of T-bet,induced
R1585 T1878 T1886 arg1Of T-bet,is
R1586 T1878 T1887 arg2Of T-bet,required
R1587 T1880 T1879 arg2Of induced,is
R1588 T1880 T1881 arg1Of induced,quickly
R1589 T1880 T1882 arg1Of induced,upon
R1590 T1880 T1885 arg1Of induced,and
R1591 T1765 T1764 arg1Of mRNA,perforin
R1592 T1884 T1882 arg2Of stimulation,upon
R1593 T1767 T1766 arg2Of expressed,are
R1594 T1884 T1883 arg1Of stimulation,TCR
R1595 T1885 T1898 arg1Of and,whereas
R1596 T1767 T1768 arg1Of expressed,by
R1597 T1767 T1782 arg1Of expressed,in
R1598 T1887 T1885 arg2Of required,and
R1599 T1887 T1886 arg2Of required,is
R1600 T1887 T1888 arg1Of required,for
R1601 T1890 T1888 arg2Of programming,for
R1602 T1890 T1889 arg1Of programming,early
R1603 T1890 T1891 arg1Of programming,of
R1604 T1893 T1891 arg2Of production,of
R1605 T1893 T1892 arg1Of production,cytokine
R1606 T1893 T1894 arg1Of production,(
R1607 T1768 T1780 arg1Of by,but
R1608 T1771 T1770 arg2Of DP,(
R1609 T1772 T1770 arg3Of ),(
R1610 T1895 T1894 arg2Of 17,(
R1611 T1896 T1894 arg3Of ),(
R1612 T1773 T1769 arg1Of thymocytes,double-positive
R1613 T1898 T1897 arg1Of whereas,","
R1614 T1899 T1900 arg1Of Eomes,is
R1615 T1899 T1901 arg2Of Eomes,induced
R1616 T1899 T1906 arg1Of Eomes,sustains
R1617 T1773 T1770 arg1Of thymocytes,(
R1618 T1773 T1774 arg1Of thymocytes,and
R1619 T1901 T1900 arg2Of induced,is
R1620 T1901 T1902 arg1Of induced,later
R1621 T1901 T1903 arg1Of induced,during
R1622 T1901 T1905 arg1Of induced,and
R1623 T1774 T1768 arg2Of and,by
R1624 T1904 T1903 arg2Of differentiation,during
R1625 T1905 T1898 arg2Of and,whereas
R1626 T1777 T1776 arg2Of SP,(
R1627 T1906 T1905 arg2Of sustains,and
R1628 T1908 T1906 arg2Of expression,sustains
R1629 T1908 T1907 arg1Of expression,IFN-γ
R1630 T1909 T1910 arg1Of Runx3,is
R1631 T1909 T1911 arg2Of Runx3,required
R1632 T1778 T1776 arg3Of ),(
R1633 T1911 T1910 arg2Of required,is
R1634 T1911 T1912 arg1Of required,for
R1635 T1911 T1918 arg1Of required,and
R1636 T1913 T1914 arg1Of Eomes,and
R1637 T1779 T1774 arg2Of thymocytes,and
R1638 T1914 T1912 arg2Of and,for
R1639 T1916 T1914 arg2Of expression,and
R1640 T1916 T1915 arg1Of expression,perforin
R1641 T1779 T1775 arg1Of thymocytes,CD8+ single-positive
R1642 T1918 T1917 arg1Of and,","
R1643 T1779 T1776 arg1Of thymocytes,(
R1644 T1782 T1780 arg2Of in,but
R1645 T1918 T1926 arg1Of and,;
R1646 T1920 T1921 arg1Of Eomes,and
R1647 T1921 T1919 arg1Of and,both
R1648 T1921 T1923 arg1Of and,bind
R1649 T1922 T1921 arg2Of Runx3,and
R1650 T1923 T1918 arg2Of bind,and
R1651 T1923 T1924 arg1Of bind,at
R1652 T1925 T1924 arg2Of the Prf1 locus,at
R1653 T1782 T1781 arg1Of in,not
R1654 T1784 T1782 arg2Of cells,in
R1655 T1928 T1927 arg2Of contrast,in
R1656 T1784 T1783 arg1Of cells,CD4+ SP
R1657 T1931 T1930 arg1Of expression,perforin
R1658 T1931 T1932 arg1Of expression,is
R1659 T1931 T1933 arg1Of expression,unaffected
R1660 T1784 T1785 arg1Of cells,(
R1661 T1932 T1926 arg2Of is,;
R1662 T1932 T1927 arg1Of is,in
R1663 T1932 T1929 arg1Of is,","
R1664 T1932 T1934 arg1Of is,in
R1665 T1933 T1932 arg2Of unaffected,is
R1666 T1786 T1785 arg2Of 14,(
R1667 T1936 T1934 arg2Of cells,in
R1668 T1787 T1785 arg3Of ),(
R1669 T1936 T1935 arg1Of cells,T-bet–deficient
R1670 T1938 T1937 arg1Of cells,T
R1671 T1938 T1939 arg1Of cells,lacking
R1672 T1938 T1953 arg1Of cells,binds
R1673 T1789 T1790 arg1Of Runx3,is
R1674 T1941 T1939 arg2Of show,lacking
R1675 T1941 T1940 arg1Of show,Runx3
R1676 T1941 T1942 arg2Of show,decreased
R1677 T1943 T1942 arg3Of expression,decreased
R1678 T1943 T1944 arg1Of expression,of
R1679 T1789 T1792 arg2Of Runx3,expressed
R1680 T1945 T1946 arg1Of IFN-γ,and
R1681 T1792 T1788 arg2Of expressed,Although
R1682 T1946 T1950 arg1Of and,and
R1683 T1948 T1946 arg2Of B,and
R1684 T1948 T1947 arg1Of B,granzyme
R1685 T1950 T1944 arg2Of and,of
R1686 T1950 T1949 arg1Of and,","
R1687 T1951 T1950 arg2Of Runx3,and
R1688 T1792 T1790 arg2Of expressed,is
R1689 T1953 T1952 arg1Of binds,also
R1690 T1792 T1791 arg1Of expressed,not
R1691 T1792 T1793 arg1Of expressed,in
R1692 T1796 T1793 arg2Of cells,in
R1693 T1956 T1953 arg2Of regions,binds
R1694 T1956 T1954 arg1Of regions,the
R1695 T1956 T1955 arg1Of regions,promoter
R1696 T1956 T1957 arg1Of regions,of
R1697 T1958 T1957 arg2Of the Ifng and Gzmb genes,of
R1698 T1962 T1961 arg1Of results,these
R1699 T1962 T1963 arg1Of results,provide
R1700 T1963 T1959 arg1Of provide,Collectively
R1701 T1963 T1960 arg1Of provide,","
R1702 T1964 T1963 arg2Of evidence,provide
R1703 T1964 T1965 arg1Of evidence,for
R1704 T1796 T1794 arg1Of cells,naive
R1705 T1796 T1795 arg1Of cells,CD4+ T
R1707 T1969 T1965 arg2Of network,for
R1708 T1969 T1966 arg1Of network,a
R1709 T1969 T1967 arg1Of network,complex
R1710 T1969 T1968 arg1Of network,transcriptional
R1711 T1969 T1970 arg2Of network,in
R1712 T1969 T1971 arg1Of network,which
R1713 T1972 T1973 arg1Of Runx3,is
R1714 T1972 T1979 arg1Of Runx3,synergizes
R1715 T1973 T1978 arg1Of is,but
R1718 T1977 T1973 arg2Of of Gzmb expression,is
R1719 T1977 T1974 arg1Of of Gzmb expression,a
R1720 T1977 T1975 arg1Of of Gzmb expression,primary
R1721 T1977 T1976 arg1Of of Gzmb expression,regulator
R1722 T1978 T1970 arg1Of but,in
R1723 T1978 T1986 arg1Of but,","
R1724 T1978 T1987 modOf but,to
R1726 T1979 T1978 arg2Of synergizes,but
R1727 T1979 T1980 arg1Of synergizes,with
R1728 T1979 T1984 arg1Of synergizes,","
R1729 T1979 T1985 arg1Of synergizes,respectively
R1731 T1981 T1982 arg1Of T-bet,and
R1732 T1982 T1980 arg2Of and,with
R1733 T1983 T1982 arg2Of Eomes,and
R1735 T1988 T1987 arg1Of promote,to
R1736 T1989 T1988 arg2Of transcription,promote
R1737 T1989 T1990 arg1Of transcription,of
R1738 T1991 T1990 arg2Of the Ifng and Prf1 genes,of
R2286 T2916 T2913 arg1Of system,A
R2287 T2916 T2914 arg1Of system,cell
R2288 T2916 T2915 arg1Of system,culture
R2289 T2916 T2917 modOf system,to
R2290 T2918 T2917 arg1Of monitor,to
R2291 T2921 T2918 arg2Of differentiation,monitor
R2292 T2921 T2919 arg1Of differentiation,effector
R2293 T2921 T2920 arg1Of differentiation,CTL
R2294 T2922 T2923 arg1Of We,used
R2295 T2922 T2930 arg1Of We,examine
R2296 T2928 T2923 arg2Of system,used
R2297 T2928 T2924 arg1Of system,a
R2298 T2928 T2925 arg1Of system,simple
R2299 T2928 T2926 arg1Of system,cell
R2300 T2928 T2927 arg1Of system,culture
R2301 T2930 T2923 arg3Of examine,used
R2302 T2930 T2929 arg1Of examine,to
R2303 T2932 T2930 arg2Of kinetics,examine
R2304 T2932 T2931 arg1Of kinetics,the
R2305 T2932 T2933 arg1Of kinetics,of
R2306 T2932 T2937 arg1Of kinetics,during
R2307 T2936 T2933 arg2Of expression,of
R2308 T2936 T2934 arg1Of expression,effector
R2309 T2936 T2935 arg1Of expression,gene
R2310 T2941 T2937 arg2Of differentiation,during
R2311 T2941 T2938 arg1Of differentiation,CD8+
R2312 T2941 T2939 arg1Of differentiation,T
R2313 T2941 T2940 arg1Of differentiation,cell
R2314 T2945 T2942 arg1Of cells,Naive
R2315 T2945 T2943 arg1Of cells,CD8+
R2316 T2945 T2944 arg1Of cells,T
R2317 T2945 T2946 arg1Of cells,from
R2318 T2945 T2951 arg1Of cells,were
R2319 T2945 T2952 arg2Of cells,activated
R2320 T2945 T2972 arg1Of cells,were
R2321 T2945 T2973 arg2Of cells,cultured
R2322 T2950 T2946 arg2Of mice,from
R2323 T2950 T2947 arg1Of mice,P14
R2324 T2950 T2948 arg1Of mice,TCR
R2325 T2950 T2949 arg1Of mice,transgenic
R2326 T2952 T2951 arg2Of activated,were
R2327 T2952 T2953 arg1Of activated,for
R2328 T2952 T2956 arg1Of activated,with
R2329 T2952 T2961 arg1Of activated,with
R2330 T2952 T2964 arg1Of activated,in
R2331 T2952 T2971 arg1Of activated,and
R2332 T2955 T2953 arg2Of d,for
R2333 T2955 T2954 arg1Of d,2
R2334 T2956 T2960 arg1Of with,or
R2335 T2957 T2958 arg1Of anti-CD3,and
R2336 T2958 T2956 arg2Of and,with
R2337 T2959 T2958 arg2Of anti-CD28,and
R2338 T2961 T2960 arg2Of with,or
R2339 T2963 T2961 arg2Of APCs,with
R2340 T2963 T2962 arg1Of APCs,splenic
R2341 T2966 T2964 arg2Of presence,in
R2342 T2966 T2965 arg1Of presence,the
R2343 T2966 T2967 arg1Of presence,of
R2344 T2969 T2967 arg2Of peptide,of
R2345 T2969 T2968 arg1Of peptide,Gp33
R2346 T2971 T2970 arg1Of and,","
R2347 T2973 T2971 arg2Of cultured,and
R2348 T2973 T2972 arg2Of cultured,were
R2349 T2973 T2974 arg1Of cultured,in
R2350 T2975 T2974 arg2Of media,in
R2351 T2975 T2976 arg1Of media,containing
R2352 T2978 T2976 arg2Of U/ml,containing
R2353 T2978 T2977 arg1Of U/ml,100
R2354 T2978 T2979 arg1Of U/ml,of
R2355 T2982 T2979 arg2Of IL-2,of
R2356 T2982 T2980 arg1Of IL-2,recombinant
R2357 T2982 T2981 arg1Of IL-2,human
R2358 T2982 T2983 arg1Of IL-2,(
R2359 T2984 T2983 arg2Of rhIL-2,(
R2360 T2985 T2983 arg3Of ),(
R2361 T2986 T2987 arg1Of We,used
R2362 T2987 T2994 arg1Of used,because
R2363 T2987 T3008 arg1Of used,;
R2364 T2990 T2987 arg2Of mice,used
R2365 T2990 T2988 arg1Of mice,TCR
R2366 T2990 T2989 arg1Of mice,transgenic
R2367 T2990 T2991 arg1Of mice,for
R2368 T2993 T2991 arg2Of experiments,for
R2369 T2993 T2992 arg1Of experiments,these
R2370 T2995 T2996 arg1Of they,provide
R2371 T2996 T2994 arg2Of provide,because
R2372 T2999 T2996 arg2Of source,provide
R2373 T2999 T2997 arg1Of source,a
R2374 T2999 T2998 arg1Of source,reliable
R2375 T2999 T3000 arg1Of source,of
R2376 T3003 T3000 arg2Of cells,of
R2377 T3003 T3001 arg1Of cells,CD8+
R2378 T3003 T3002 arg1Of cells,T
R2379 T3003 T3004 arg1Of cells,that
R2380 T3003 T3005 arg1Of cells,are
R2381 T3003 T3007 arg1Of cells,naive
R2382 T3007 T3005 arg2Of naive,are
R2383 T3007 T3006 arg1Of naive,truly
R2384 T3008 T3009 arg1Of ;,however
R2385 T3011 T3012 arg1Of we,chose
R2386 T3011 T3015 arg1Of we,stimulate
R2387 T3012 T3008 arg2Of chose,;
R2388 T3012 T3010 arg1Of chose,","
R2389 T3012 T3023 arg1Of chose,as
R2390 T3015 T3012 arg2Of stimulate,chose
R2391 T3015 T3013 arg1Of stimulate,not
R2392 T3015 T3014 arg1Of stimulate,to
R2393 T3015 T3019 arg1Of stimulate,in
R2394 T3016 T3015 arg2Of cells,stimulate
R2395 T3016 T3017 arg1Of cells,with
R2396 T3018 T3017 arg2Of antigen,with
R2397 T3021 T3019 arg2Of experiments,in
R2398 T3021 T3020 arg1Of experiments,most
R2399 T3023 T3022 arg1Of as,so
R2400 T3025 T3023 arg2Of avoid,as
R2401 T3025 T3024 arg1Of avoid,to
R2402 T3026 T3025 arg2Of contamination,avoid
R2403 T3026 T3027 arg1Of contamination,with
R2404 T3028 T3029 arg1Of proteins,and
R2405 T3029 T3027 arg2Of and,with
R2406 T3029 T3032 arg2Of and,derived
R2407 T3031 T3029 arg2Of acids,and
R2408 T3031 T3030 arg1Of acids,nucleic
R2409 T3032 T3033 arg1Of derived,from
R2410 T3034 T3033 arg2Of APCs,from
R2411 T3035 T3036 arg1Of There,were
R2412 T3036 T3043 arg1Of were,during
R2413 T3036 T3051 arg1Of were,and
R2414 T3038 T3037 arg1Of minor,only
R2415 T3039 T3036 arg2Of differences,were
R2416 T3039 T3038 arg1Of differences,minor
R2417 T3039 T3040 arg1Of differences,in
R2418 T3042 T3040 arg2Of expression,in
R2419 T3042 T3041 arg1Of expression,gene
R2420 T3044 T3043 arg2Of differentiation,during
R2421 T3044 T3045 arg2Of differentiation,induced
R2422 T3047 T3045 arg1Of antigen/APC,induced
R2423 T3047 T3046 arg2Of antigen/APC,by
R2424 T3047 T3048 arg1Of antigen/APC,versus
R2425 T3049 T3048 arg2Of anti-CD3/anti-CD28,versus
R2426 T3051 T3050 arg1Of and,","
R2427 T3054 T3052 arg1Of conclusions,the
R2428 T3054 T3053 arg1Of conclusions,major
R2429 T3054 T3055 arg2Of conclusions,presented
R2430 T3054 T3059 arg1Of conclusions,are
R2431 T3055 T3056 arg1Of presented,in
R2432 T3058 T3056 arg2Of report,in
R2433 T3058 T3057 arg1Of report,this
R2434 T3059 T3051 arg2Of are,and
R2435 T3059 T3062 arg1Of are,for
R2436 T3061 T3059 arg2Of same,are
R2437 T3061 T3060 arg1Of same,the
R2438 T3065 T3062 arg2Of conditions,for
R2439 T3065 T3063 arg1Of conditions,both
R2440 T3065 T3064 arg1Of conditions,activating
R2441 T3069 T3066 arg2Of conditions,Under
R2442 T3069 T3067 arg1Of conditions,our
R2443 T3069 T3068 arg1Of conditions,culture
R2444 T3074 T3071 arg2Of cells,activated
R2445 T3074 T3072 arg1Of cells,CD8+
R2446 T3074 T3073 arg1Of cells,T
R2447 T3074 T3075 arg1Of cells,expanded
R2448 T3074 T3078 arg2Of cells,accumulated
R2449 T3075 T3076 arg1Of expanded,exponentially
R2450 T3075 T3077 arg1Of expanded,and
R2451 T3077 T3066 arg1Of and,Under
R2452 T3077 T3070 arg1Of and,","
R2453 T3078 T3077 arg2Of accumulated,and
R2454 T3078 T3079 arg1Of accumulated,for
R2455 T3081 T3080 arg1Of 8,>
R2456 T3082 T3079 arg2Of d,for
R2457 T3082 T3081 arg1Of d,8
R2458 T3083 T3084 arg1Of We,limited
R2459 T3084 T3087 arg1Of limited,to
R2460 T3084 T3092 arg1Of limited,after
R2461 T3086 T3084 arg2Of analysis,limited
R2462 T3086 T3085 arg1Of analysis,our
R2463 T3091 T3087 arg2Of d,to
R2464 T3091 T3088 arg1Of d,the
R2465 T3091 T3089 arg1Of d,first
R2466 T3091 T3090 arg1Of d,6–8
R2467 T3093 T3092 arg2Of activation,after
R2468 T3093 T3094 arg1Of activation,","
R2469 T3096 T3094 arg2Of period,","
R2470 T3096 T3095 arg1Of period,a
R2471 T3096 T3097 arg1Of period,that
R2472 T3096 T3098 arg1Of period,coincides
R2473 T3098 T3099 arg1Of coincides,with
R2474 T3098 T3106 arg1Of coincides,after
R2475 T3101 T3099 arg2Of expansion,with
R2476 T3101 T3100 arg1Of expansion,clonal
R2477 T3101 T3102 arg1Of expansion,of
R2478 T3105 T3102 arg2Of cells,of
R2479 T3105 T3103 arg1Of cells,CD8+
R2480 T3105 T3104 arg1Of cells,T
R2481 T3107 T3106 arg2Of activation,after
R2482 T3107 T3109 arg1Of activation,vivo
R2483 T3109 T3108 arg1Of vivo,in
R3009 T3827 T3825 arg1Of kinetics,Distinct
R3010 T3827 T3826 arg1Of kinetics,expression
R3011 T3827 T3828 arg1Of kinetics,of
R3012 T3829 T3830 arg1Of perforin,and
R3013 T3830 T3828 arg2Of and,of
R3014 T3830 T3833 arg1Of and,during
R3015 T3832 T3830 arg2Of B,and
R3016 T3832 T3831 arg1Of B,granzyme
R3017 T3835 T3833 arg2Of development,during
R3018 T3835 T3834 arg1Of development,CTL
R3019 T3835 T3836 arg1Of development,in
R3020 T3837 T3836 arg2Of culture,in
R3021 T3839 T3838 arg1Of experiments,Our
R3022 T3839 T3840 arg1Of experiments,revealed
R3023 T3842 T3840 arg2Of differences,revealed
R3024 T3842 T3841 arg1Of differences,clear
R3025 T3842 T3843 arg1Of differences,in
R3026 T3842 T3855 arg1Of differences,during
R3027 T3845 T3843 arg2Of kinetics,in
R3028 T3845 T3844 arg1Of kinetics,the
R3029 T3845 T3846 arg1Of kinetics,of
R3030 T3847 T3848 arg1Of perforin,","
R3031 T3848 T3852 arg1Of ",",and
R3032 T3850 T3848 arg2Of B,","
R3033 T3850 T3849 arg1Of B,granzyme
R3034 T3852 T3846 arg2Of and,of
R3035 T3852 T3851 arg1Of and,","
R3036 T3854 T3852 arg2Of expression,and
R3037 T3854 T3853 arg1Of expression,cytokine
R3038 T3859 T3855 arg2Of activation,during
R3039 T3859 T3856 arg1Of activation,CD8+
R3040 T3859 T3857 arg1Of activation,T
R3041 T3859 T3858 arg1Of activation,cell
R3042 T3859 T3860 arg1Of activation,(
R3043 T3861 T3860 arg2Of Fig.,(
R3044 T3861 T3862 arg1Of Fig.,1
R3045 T3863 T3860 arg3Of ),(
R3046 T3866 T3864 arg1Of cells,Naive
R3047 T3866 T3865 arg1Of cells,T
R3048 T3866 T3867 arg1Of cells,showed
R3049 T3869 T3868 arg1Of expression,detectable
R3050 T3869 T3870 arg1Of expression,of
R3051 T3869 T3875 arg1Of expression,as
R3052 T3872 T3870 arg2Of mRNA,of
R3053 T3872 T3871 arg1Of mRNA,perforin
R3054 T3875 T3867 arg2Of as,showed
R3055 T3875 T3873 arg1Of as,as
R3056 T3875 T3874 arg1Of as,well
R3057 T3877 T3875 arg2Of protein,as
R3058 T3877 T3876 arg1Of protein,perforin
R3059 T3877 T3878 arg1Of protein,(
R3060 T3879 T3878 arg2Of Fig.,(
R3061 T3879 T3880 arg1Of Fig.,1
R3062 T3879 T3881 arg1Of Fig.,","
R3063 T3882 T3881 arg2Of A–D,","
R3064 T3883 T3878 arg3Of ),(
R3065 T3885 T3884 arg1Of to,Relative
R3066 T3887 T3885 arg2Of expression,to
R3067 T3887 T3886 arg1Of expression,its
R3068 T3887 T3888 arg1Of expression,in
R3069 T3891 T3888 arg2Of cells,in
R3070 T3891 T3889 arg1Of cells,naive
R3071 T3891 T3890 arg1Of cells,T
R3072 T3895 T3894 arg2Of Prf1,(
R3073 T3896 T3894 arg3Of ),(
R3074 T3898 T3893 arg1Of expression,perforin
R3075 T3898 T3894 arg1Of expression,(
R3076 T3898 T3897 arg1Of expression,mRNA
R3077 T3898 T3899 arg1Of expression,did
R3078 T3898 T3901 arg1Of expression,increase
R3079 T3898 T3907 arg1Of expression,showed
R3080 T3901 T3899 arg2Of increase,did
R3081 T3901 T3900 arg1Of increase,not
R3082 T3901 T3902 arg1Of increase,appreciably
R3083 T3901 T3903 arg1Of increase,at
R3084 T3901 T3906 arg1Of increase,but
R3085 T3904 T3903 arg2Of day,at
R3086 T3904 T3905 arg1Of day,2
R3087 T3906 T3885 arg1Of but,to
R3088 T3906 T3892 arg1Of but,","
R3089 T3906 T3914 arg1Of but,","
R3090 T3906 T3915 modOf but,followed
R3091 T3907 T3906 arg2Of showed,but
R3092 T3910 T3907 arg2Of decrease,showed
R3093 T3910 T3908 arg1Of decrease,a
R3094 T3910 T3909 arg1Of decrease,reproducible
R3095 T3910 T3911 arg1Of decrease,at
R3096 T3912 T3911 arg2Of day,at
R3097 T3912 T3913 arg1Of day,4
R3098 T3918 T3915 arg1Of reexpression,followed
R3099 T3918 T3916 arg2Of reexpression,by
R3100 T3918 T3917 arg1Of reexpression,robust
R3101 T3918 T3919 arg1Of reexpression,between
R3102 T3921 T3922 arg1Of 4,and
R3103 T3922 T3919 arg2Of and,between
R3104 T3922 T3920 arg1Of and,days
R3105 T3922 T3924 arg1Of and,(
R3106 T3923 T3922 arg2Of 8,and
R3107 T3925 T3924 arg2Of Fig.,(
R3108 T3925 T3926 arg1Of Fig.,1
R3109 T3925 T3927 arg1Of Fig.,","
R3110 T3928 T3927 arg2Of A–D,","
R3111 T3929 T3924 arg3Of ),(
R3112 T3931 T3930 arg2Of contrast,In
R3113 T3936 T3935 arg2Of Gzmb,(
R3114 T3937 T3935 arg3Of ),(
R3115 T3938 T3933 arg1Of mRNA,granzyme
R3116 T3938 T3934 arg1Of mRNA,B
R3117 T3938 T3935 arg1Of mRNA,(
R3118 T3938 T3939 arg1Of mRNA,was
R3119 T3938 T3940 arg1Of mRNA,low
R3120 T3938 T3942 arg1Of mRNA,undetectable
R3121 T3938 T3948 arg1Of mRNA,was
R3122 T3938 T3950 arg2Of mRNA,up-regulated
R3123 T3938 T3957 arg2Of mRNA,increased
R3124 T3939 T3947 arg1Of was,but
R3125 T3940 T3941 arg1Of low,or
R3126 T3941 T3939 arg2Of or,was
R3127 T3941 T3943 arg1Of or,in
R3128 T3942 T3941 arg2Of undetectable,or
R3129 T3946 T3943 arg2Of cells,in
R3130 T3946 T3944 arg1Of cells,naive
R3131 T3946 T3945 arg1Of cells,T
R3132 T3947 T3930 arg1Of but,In
R3133 T3947 T3932 arg1Of but,","
R3134 T3950 T3951 arg1Of up-regulated,by
R3135 T3950 T3956 arg1Of up-regulated,and
R3136 T3952 T3951 arg2Of day,by
R3137 T3952 T3953 arg1Of day,2
R3138 T3952 T3954 arg1Of day,after
R3139 T3955 T3954 arg2Of stimulation,after
R3140 T3956 T3947 arg2Of and,but
R3141 T3956 T3948 arg2Of and,was
R3142 T3956 T3949 arg1Of and,strongly
R3143 T3957 T3956 arg2Of increased,and
R3144 T3957 T3958 arg1Of increased,progressively
R3145 T3957 T3959 arg1Of increased,until
R3146 T3957 T3970 arg1Of increased,;
R3147 T3960 T3959 arg2Of day,until
R3148 T3960 T3961 arg1Of day,6
R3149 T3960 T3962 arg1Of day,(
R3150 T3963 T3962 arg2Of Fig.,(
R3151 T3963 T3964 arg1Of Fig.,1
R3152 T3963 T3965 arg1Of Fig.,","
R3153 T3966 T3967 arg1Of A,and
R3154 T3967 T3965 arg2Of and,","
R3155 T3968 T3967 arg2Of B,and
R3156 T3969 T3962 arg3Of ),(
R3157 T3975 T3973 arg1Of protein,granzyme
R3158 T3975 T3974 arg1Of protein,B
R3159 T3975 T3976 arg1Of protein,was
R3160 T3975 T3977 arg2Of protein,expressed
R3161 T3975 T3982 arg1Of protein,remained
R3162 T3975 T3983 arg1Of protein,high
R3163 T3977 T3976 arg2Of expressed,was
R3164 T3977 T3978 arg1Of expressed,by
R3165 T3977 T3981 arg1Of expressed,and
R3166 T3979 T3978 arg2Of day,by
R3167 T3979 T3980 arg1Of day,4
R3168 T3981 T3970 arg2Of and,;
R3169 T3981 T3971 arg1Of and,similarly
R3170 T3981 T3972 arg1Of and,","
R3171 T3982 T3981 arg2Of remained,and
R3172 T3983 T3982 arg2Of high,remained
R3173 T3983 T3984 arg1Of high,until
R3174 T3985 T3984 arg2Of day,until
R3175 T3985 T3986 arg1Of day,6
R3176 T3985 T3987 arg1Of day,(
R3177 T3990 T3987 arg2Of E,(
R3178 T3990 T3988 arg1Of E,Fig.
R3179 T3990 T3989 arg1Of E,1
R3180 T3991 T3987 arg3Of ),(
R3181 T3993 T3992 arg2Of expected,As
R3182 T3997 T3995 arg1Of fraction,a
R3183 T3997 T3996 arg1Of fraction,small
R3184 T3997 T3998 arg1Of fraction,of
R3185 T3997 T4002 arg1Of fraction,expressed
R3186 T4001 T3998 arg2Of cells,of
R3187 T4001 T3999 arg1Of cells,naive
R3188 T4001 T4000 arg1Of cells,T
R3189 T4002 T4008 arg1Of expressed,in
R3190 T4002 T4013 arg1Of expressed,and
R3191 T4005 T4006 arg1Of IFN-γ,and
R3192 T4006 T4002 arg2Of and,expressed
R3193 T4006 T4003 arg1Of and,the
R3194 T4006 T4004 arg1Of and,cytokines
R3195 T4007 T4006 arg2Of TNF,and
R3196 T4008 T4010 arg1Of in,to
R3197 T4009 T4008 arg2Of response,in
R3198 T4011 T4008 arg3Of stimulation,in
R3199 T4013 T3992 arg1Of and,As
R3200 T4013 T3994 arg1Of and,","
R3201 T4013 T4012 arg1Of and,","
R3202 T4015 T4014 arg1Of capacity,this
R3203 T4015 T4016 arg1Of capacity,increased
R3204 T4016 T4013 arg2Of increased,and
R3205 T4016 T4017 arg1Of increased,significantly
R3206 T4016 T4018 arg1Of increased,in
R3207 T4016 T4021 arg1Of increased,(
R3208 T4020 T4018 arg2Of cells,in
R3209 T4020 T4019 arg2Of cells,differentiated
R3210 T4024 T4021 arg2Of E,(
R3211 T4024 T4022 arg1Of E,Fig.
R3212 T4024 T4023 arg1Of E,1
R3213 T4024 T4026 modOf E,see
R3214 T4026 T4025 arg1Of see,;
R3215 T4026 T4027 arg1Of see,also
R3216 T4028 T4029 arg1Of Fig.,2
R3217 T4030 T4026 arg2Of A,see
R3218 T4030 T4028 arg1Of A,Fig.
R3219 T4031 T4021 arg3Of ),(
R3220 T4032 T4033 arg1Of We,evaluated
R3221 T4033 T4037 arg1Of evaluated,in
R3222 T4036 T4033 arg2Of function,evaluated
R3223 T4036 T4034 arg1Of function,antigen-dependent
R3224 T4036 T4035 arg1Of function,cytolytic
R3225 T4040 T4037 arg2Of assay,in
R3226 T4040 T4038 arg1Of assay,a
R3227 T4040 T4039 arg1Of assay,short-term
R3228 T4040 T4041 arg2Of assay,in
R3229 T4040 T4042 arg1Of assay,which
R3230 T4045 T4043 arg1Of death,target
R3231 T4045 T4044 arg1Of death,cell
R3232 T4045 T4046 arg1Of death,was
R3233 T4045 T4047 arg2Of death,measured
R3234 T4047 T4041 arg1Of measured,in
R3235 T4047 T4046 arg2Of measured,was
R3236 T4047 T4048 arg1Of measured,within
R3237 T4050 T4048 arg2Of h,within
R3238 T4050 T4049 arg1Of h,2
R3239 T4050 T4051 arg1Of h,(
R3240 T4054 T4051 arg2Of F,(
R3241 T4054 T4052 arg1Of F,Fig.
R3242 T4054 T4053 arg1Of F,1
R3243 T4055 T4051 arg3Of ),(
R3244 T4057 T4056 arg2Of limiting,By
R3245 T4059 T4057 arg2Of duration,limiting
R3246 T4059 T4058 arg1Of duration,the
R3247 T4059 T4060 arg1Of duration,of
R3248 T4062 T4060 arg2Of stimulation,of
R3249 T4062 T4061 arg1Of stimulation,TCR
R3250 T4065 T4057 arg1Of strategy,limiting
R3251 T4065 T4064 arg1Of strategy,this
R3252 T4065 T4066 arg1Of strategy,minimizes
R3253 T4066 T4056 arg1Of minimizes,By
R3254 T4066 T4063 arg1Of minimizes,","
R3255 T4066 T4073 arg1Of minimizes,during
R3256 T4067 T4066 arg2Of cytolysis,minimizes
R3257 T4067 T4068 arg1Of cytolysis,secondary
R3258 T4068 T4069 arg1Of secondary,to
R3259 T4072 T4069 arg2Of expression,to
R3260 T4072 T4070 arg1Of expression,new
R3261 T4072 T4071 arg1Of expression,gene
R3262 T4075 T4073 arg2Of period,during
R3263 T4075 T4074 arg1Of period,the
R3264 T4075 T4076 arg1Of period,of
R3265 T4078 T4076 arg2Of assay,of
R3266 T4078 T4077 arg1Of assay,the
R3267 T4081 T4079 arg1Of cells,Naive
R3268 T4081 T4080 arg1Of cells,T
R3269 T4081 T4082 arg1Of cells,did
R3270 T4081 T4084 arg1Of cells,display
R3271 T4084 T4082 arg2Of display,did
R3272 T4084 T4083 arg1Of display,not
R3273 T4084 T4088 arg1Of display,in
R3274 T4084 T4096 arg1Of display,","
R3275 T4084 T4099 arg1Of display,because
R3276 T4086 T4085 arg1Of cytolytic,significant
R3277 T4087 T4084 arg2Of function,display
R3278 T4087 T4086 arg1Of function,cytolytic
R3279 T4091 T4088 arg2Of assay,in
R3280 T4091 T4089 arg1Of assay,this
R3281 T4091 T4090 arg1Of assay,short-term
R3282 T4091 T4092 arg1Of assay,(
R3283 T4094 T4092 arg2Of data,(
R3284 T4094 T4093 arg1Of data,unpublished
R3285 T4095 T4092 arg3Of ),(
R3286 T4098 T4097 arg1Of likely,most
R3287 T4099 T4098 arg1Of because,likely
R3288 T4100 T4101 arg1Of they,express
R3289 T4100 T4110 arg1Of they,lack
R3290 T4101 T4109 arg1Of express,and
R3291 T4102 T4103 arg1Of immature,(
R3292 T4104 T4103 arg2Of unprocessed,(
R3293 T4105 T4103 arg3Of ),(
R3294 T4106 T4101 arg2Of forms,express
R3295 T4106 T4102 arg1Of forms,immature
R3296 T4106 T4107 arg1Of forms,of
R3297 T4108 T4107 arg2Of perforin,of
R3298 T4109 T4099 arg2Of and,because
R3299 T4110 T4109 arg2Of lack,and
R3300 T4110 T4115 arg1Of lack,(
R3301 T4112 T4110 arg2Of capacity,lack
R3302 T4112 T4111 arg1Of capacity,the
R3303 T4112 T4113 modOf capacity,to
R3304 T4114 T4113 arg1Of degranulate,to
R3305 T4116 T4115 arg2Of 18,(
R3306 T4116 T4117 arg1Of 18,","
R3307 T4118 T4117 arg2Of 19,","
R3308 T4119 T4115 arg3Of ),(
R3309 T4121 T4120 arg1Of after,Even
R3310 T4122 T4121 arg2Of activation,after
R3311 T4122 T4123 arg1Of activation,for
R3312 T4124 T4125 arg1Of 2,or
R3313 T4126 T4125 arg2Of 4,or
R3314 T4127 T4123 arg2Of d,for
R3315 T4127 T4124 arg1Of d,2
R3316 T4127 T4126 arg1Of d,4
R3317 T4130 T4129 arg1Of cells,the
R3318 T4130 T4131 arg1Of cells,showed
R3319 T4131 T4121 arg1Of showed,after
R3320 T4131 T4128 arg1Of showed,","
R3321 T4133 T4132 arg1Of cytolytic,poor
R3322 T4134 T4131 arg2Of activity,showed
R3323 T4134 T4133 arg1Of activity,cytolytic
R3324 T4134 T4135 arg1Of activity,(
R3325 T4134 T4140 arg1Of activity,","
R3326 T4134 T4141 arg1Of activity,in
R3327 T4138 T4135 arg2Of F,(
R3328 T4138 T4136 arg1Of F,Fig.
R3329 T4138 T4137 arg1Of F,1
R3330 T4139 T4135 arg3Of ),(
R3331 T4143 T4141 arg2Of contrast,in
R3332 T4143 T4142 arg1Of contrast,striking
R3333 T4143 T4144 arg1Of contrast,to
R3334 T4146 T4144 arg2Of capacity,to
R3335 T4146 T4145 arg1Of capacity,their
R3336 T4146 T4147 arg1Of capacity,for
R3337 T4150 T4147 arg2Of production,for
R3338 T4150 T4148 arg1Of production,efficient
R3339 T4150 T4149 arg1Of production,cytokine
R3340 T4150 T4151 arg1Of production,(
R3341 T4154 T4151 arg2Of E,(
R3342 T4154 T4152 arg1Of E,Fig.
R3343 T4154 T4153 arg1Of E,1
R3344 T4155 T4151 arg3Of ),(
R3345 T4157 T4156 arg1Of cells,Only
R3346 T4157 T4158 arg2Of cells,cultured
R3347 T4157 T4162 arg1Of cells,displayed
R3348 T4158 T4159 arg1Of cultured,until
R3349 T4160 T4159 arg2Of day,until
R3350 T4160 T4161 arg1Of day,6
R3351 T4162 T4165 arg1Of displayed,","
R3352 T4162 T4166 arg1Of displayed,as
R3353 T4164 T4162 arg2Of cytotoxicity,displayed
R3354 T4164 T4163 arg1Of cytotoxicity,robust
R3355 T4167 T4166 arg2Of judged,as
R3356 T4170 T4167 arg1Of ability,judged
R3357 T4170 T4168 arg2Of ability,by
R3358 T4170 T4169 arg1Of ability,their
R3359 T4170 T4171 modOf ability,to
R3360 T4170 T4181 arg1Of ability,(
R3361 T4172 T4171 arg1Of induce,to
R3362 T4172 T4174 arg1Of induce,in
R3363 T4173 T4172 arg2Of apoptosis,induce
R3364 T4177 T4174 arg2Of number,in
R3365 T4177 T4175 arg1Of number,a
R3366 T4177 T4176 arg1Of number,large
R3367 T4177 T4178 arg1Of number,of
R3368 T4180 T4178 arg2Of cells,of
R3369 T4180 T4179 arg1Of cells,target
R3370 T4184 T4181 arg2Of F,(
R3371 T4184 T4182 arg1Of F,Fig.
R3372 T4184 T4183 arg1Of F,1
R3373 T4185 T4181 arg3Of ),(
R3374 T4187 T4186 arg1Of results,These
R3375 T4187 T4188 arg1Of results,show
R3376 T4194 T4190 arg2Of stimulus,after
R3377 T4194 T4191 arg1Of stimulus,a
R3378 T4194 T4192 arg1Of stimulus,strong
R3379 T4194 T4193 arg1Of stimulus,priming
R3380 T4194 T4195 arg1Of stimulus,through
R3381 T4196 T4197 arg1Of TCRs,and
R3382 T4197 T4195 arg2Of and,through
R3383 T4197 T4201 arg1Of and,vitro
R3384 T4199 T4197 arg2Of receptors,and
R3385 T4199 T4198 arg1Of receptors,co-stimulatory
R3386 T4201 T4200 arg1Of vitro,in
R3387 T4205 T4203 arg1Of expression,granzyme
R3388 T4205 T4204 arg1Of expression,B
R3389 T4205 T4206 arg1Of expression,and
R3390 T4206 T4213 arg1Of and,are
R3391 T4206 T4214 arg2Of and,programmed
R3392 T4208 T4206 arg2Of ability,and
R3393 T4208 T4207 arg1Of ability,the
R3394 T4208 T4209 modOf ability,to
R3395 T4210 T4209 arg1Of produce,to
R3396 T4212 T4210 arg2Of cytokines,produce
R3397 T4212 T4211 arg1Of cytokines,effector
R3398 T4214 T4188 arg2Of programmed,show
R3399 T4214 T4189 arg1Of programmed,that
R3400 T4214 T4190 arg1Of programmed,after
R3401 T4214 T4202 arg1Of programmed,","
R3402 T4214 T4213 arg2Of programmed,are
R3403 T4214 T4215 arg1Of programmed,early
R3404 T4214 T4216 arg1Of programmed,","
R3405 T4214 T4217 arg1Of programmed,whereas
R3406 T4214 T4226 arg1Of programmed,","
R3407 T4214 T4227 arg1Of programmed,during
R3408 T4219 T4218 arg1Of expression,perforin
R3409 T4219 T4220 arg1Of expression,and
R3410 T4220 T4223 arg1Of and,are
R3411 T4220 T4224 arg2Of and,induced
R3412 T4222 T4220 arg2Of function,and
R3413 T4222 T4221 arg1Of function,cytolytic
R3414 T4224 T4217 arg2Of induced,whereas
R3415 T4224 T4223 arg2Of induced,are
R3416 T4224 T4225 arg1Of induced,later
R3417 T4229 T4227 arg2Of phase,during
R3418 T4229 T4228 arg1Of phase,the
R3419 T4229 T4230 arg1Of phase,of
R3420 T4232 T4230 arg2Of expansion,of
R3421 T4232 T4231 arg1Of expansion,clonal
R3422 T4232 T4233 arg1Of expansion,in
R3423 T4234 T4233 arg2Of IL-2,in
R3424 T4241 T4237 arg1Of functions,the
R3425 T4241 T4238 arg1Of functions,two
R3426 T4241 T4239 arg1Of functions,major
R3427 T4241 T4240 arg1Of functions,effector
R3428 T4241 T4242 arg1Of functions,of
R3429 T4241 T4251 arg1Of functions,are
R3430 T4241 T4254 arg2Of functions,coregulated
R3431 T4243 T4244 arg1Of CTL,","
R3432 T4244 T4242 arg2Of ",",of
R3433 T4246 T4245 arg1Of production,cytokine
R3434 T4246 T4247 arg1Of production,and
R3435 T4247 T4244 arg2Of and,","
R3436 T4249 T4247 arg2Of activity,and
R3437 T4249 T4248 arg1Of activity,cytolytic
R3438 T4254 T4235 arg1Of coregulated,Therefore
R3439 T4254 T4236 arg1Of coregulated,","
R3440 T4254 T4250 arg1Of coregulated,","
R3441 T4254 T4251 arg2Of coregulated,are
R3442 T4254 T4252 arg1Of coregulated,not
R3443 T4254 T4253 arg1Of coregulated,intrinsically
R4074 T5121 T5120 arg1Of kinetics,Distinct
R4075 T5121 T5122 arg1Of kinetics,of
R4076 T5121 T5127 arg1Of kinetics,during
R4077 T5123 T5124 arg1Of T-bet,and
R4078 T5125 T5124 arg2Of Eomes,and
R4079 T5126 T5122 arg2Of expression,of
R4080 T5126 T5123 arg1Of expression,T-bet
R4081 T5126 T5125 arg1Of expression,Eomes
R4082 T5129 T5127 arg2Of differentiation,during
R4083 T5129 T5128 arg1Of differentiation,CTL
R4084 T5133 T5131 arg1Of factors,T-box
R4085 T5133 T5132 arg1Of factors,transcription
R4086 T5134 T5135 arg1Of T-bet,and
R4087 T5135 T5130 arg1Of and,The
R4088 T5135 T5133 arg1Of and,factors
R4089 T5135 T5137 arg1Of and,have
R4090 T5135 T5138 arg1Of and,been
R4091 T5135 T5139 arg2Of and,linked
R4092 T5136 T5135 arg2Of Eomes,and
R4093 T5139 T5137 arg2Of linked,have
R4094 T5139 T5138 arg2Of linked,been
R4095 T5139 T5140 arg1Of linked,to
R4096 T5142 T5140 arg2Of regulation,to
R4097 T5142 T5141 arg1Of regulation,the
R4098 T5142 T5143 arg1Of regulation,of
R4099 T5144 T5143 arg2Of genes,of
R4100 T5144 T5145 arg1Of genes,encoding
R4101 T5147 T5146 arg1Of cytokines,effector
R4102 T5147 T5148 arg1Of cytokines,(
R4103 T5147 T5153 arg1Of cytokines,and
R4104 T5151 T5148 arg2Of Ifng,(
R4105 T5151 T5149 arg1Of Ifng,e.g.
R4106 T5151 T5150 arg1Of Ifng,","
R4107 T5152 T5148 arg3Of ),(
R4108 T5153 T5145 arg2Of and,encoding
R4109 T5153 T5159 arg1Of and,(
R4110 T5153 T5166 arg1Of and,(
R4111 T5154 T5153 arg2Of genes,and
R4112 T5154 T5155 arg1Of genes,important
R4113 T5155 T5156 arg1Of important,for
R4114 T5158 T5156 arg2Of function,for
R4115 T5158 T5157 arg1Of function,cytolytic
R4116 T5162 T5163 arg1Of Prf1,and
R4117 T5163 T5159 arg2Of and,(
R4118 T5163 T5160 arg1Of and,e.g.
R4119 T5163 T5161 arg1Of and,","
R4120 T5164 T5163 arg2Of GzmB,and
R4121 T5165 T5159 arg3Of ),(
R4122 T5167 T5166 arg2Of 20,(
R4123 T5168 T5166 arg3Of ),(
R4124 T5169 T5170 arg1Of We,investigated
R4125 T5172 T5170 arg2Of kinetics,investigated
R4126 T5172 T5171 arg1Of kinetics,the
R4127 T5172 T5173 arg1Of kinetics,of
R4128 T5174 T5173 arg2Of expression,of
R4129 T5174 T5175 arg1Of expression,of
R4130 T5178 T5175 arg2Of factors,of
R4131 T5178 T5176 arg1Of factors,these
R4132 T5178 T5177 arg1Of factors,transcription
R4133 T5178 T5179 arg1Of factors,in
R4134 T5182 T5181 arg1Of vitro,in
R4135 T5183 T5179 arg2Of cultures,in
R4136 T5183 T5180 arg1Of cultures,our
R4137 T5183 T5182 arg1Of cultures,vitro
R4138 T5183 T5184 arg1Of cultures,(
R4139 T5185 T5184 arg2Of Fig.,(
R4140 T5185 T5186 arg1Of Fig.,1
R4141 T5185 T5187 arg1Of Fig.,","
R4142 T5188 T5187 arg2Of A–D,","
R4143 T5189 T5184 arg3Of ),(
R4144 T5191 T5190 arg1Of mRNA,T-bet
R4145 T5191 T5192 arg1Of mRNA,and
R4146 T5192 T5194 arg1Of and,were
R4147 T5192 T5196 arg1Of and,detectable
R4148 T5192 T5204 arg1Of and,were
R4149 T5192 T5206 arg2Of and,induced
R4150 T5192 T5215 arg1Of and,remained
R4151 T5192 T5216 arg2Of and,expressed
R4152 T5193 T5192 arg2Of protein,and
R4153 T5194 T5195 arg1Of were,not
R4154 T5194 T5203 arg1Of were,but
R4155 T5196 T5194 arg2Of detectable,were
R4156 T5196 T5197 arg1Of detectable,in
R4157 T5201 T5197 arg2Of cells,in
R4158 T5201 T5198 arg1Of cells,naive
R4159 T5201 T5199 arg1Of cells,CD8+
R4160 T5201 T5200 arg1Of cells,T
R4161 T5203 T5202 arg1Of but,","
R4162 T5203 T5214 arg1Of but,and
R4163 T5206 T5203 arg2Of induced,but
R4164 T5206 T5204 arg2Of induced,were
R4165 T5206 T5205 arg1Of induced,strongly
R4166 T5206 T5207 arg1Of induced,upon
R4167 T5209 T5207 arg2Of priming,upon
R4168 T5209 T5208 arg1Of priming,TCR
R4169 T5209 T5210 arg1Of priming,(
R4170 T5211 T5210 arg2Of day,(
R4171 T5211 T5212 arg1Of day,2
R4172 T5213 T5210 arg3Of ),(
R4173 T5216 T5214 arg2Of expressed,and
R4174 T5216 T5215 arg2Of expressed,remained
R4175 T5216 T5217 arg1Of expressed,through
R4176 T5218 T5217 arg2Of day,through
R4177 T5218 T5219 arg1Of day,6
R4178 T5218 T5220 arg1Of day,of
R4179 T5221 T5220 arg2Of differentiation,of
R4180 T5221 T5222 arg1Of differentiation,(
R4181 T5223 T5222 arg2Of Fig.,(
R4182 T5223 T5224 arg1Of Fig.,1
R4183 T5223 T5225 arg1Of Fig.,","
R4184 T5223 T5234 arg1Of Fig.,","
R4185 T5226 T5227 arg1Of A,and
R4186 T5227 T5225 arg2Of and,","
R4187 T5227 T5230 arg2Of and,quantified
R4188 T5228 T5227 arg2Of C,and
R4189 T5230 T5229 arg1Of quantified,;
R4190 T5230 T5231 arg1Of quantified,in
R4191 T5232 T5231 arg2Of Fig.,in
R4192 T5232 T5233 arg1Of Fig.,1
R4193 T5235 T5236 arg1Of B,and
R4194 T5236 T5234 arg2Of and,","
R4195 T5237 T5236 arg2Of D,and
R4196 T5238 T5222 arg3Of ),(
R4197 T5240 T5239 arg2Of contrast,In
R4198 T5243 T5242 arg1Of expression,Eomes
R4199 T5243 T5244 arg1Of expression,was
R4200 T5243 T5245 arg1Of expression,low
R4201 T5243 T5247 arg1Of expression,undetectable
R4202 T5244 T5239 arg1Of was,In
R4203 T5244 T5241 arg1Of was,","
R4204 T5244 T5248 arg1Of was,at
R4205 T5244 T5261 arg1Of was,and
R4206 T5245 T5246 arg1Of low,or
R4207 T5246 T5244 arg2Of or,was
R4208 T5247 T5246 arg2Of undetectable,or
R4209 T5251 T5252 arg1Of mRNA,and
R4210 T5253 T5252 arg2Of protein,and
R4211 T5254 T5248 arg2Of levels,at
R4212 T5254 T5249 arg1Of levels,both
R4213 T5254 T5250 arg1Of levels,the
R4214 T5254 T5251 arg1Of levels,mRNA
R4215 T5254 T5253 arg1Of levels,protein
R4216 T5254 T5255 arg1Of levels,in
R4217 T5259 T5255 arg2Of cells,in
R4218 T5259 T5256 arg1Of cells,naive
R4219 T5259 T5257 arg1Of cells,CD8+
R4220 T5259 T5258 arg1Of cells,T
R4221 T5261 T5260 arg1Of and,","
R4222 T5263 T5262 arg1Of priming,TCR
R4223 T5263 T5264 arg1Of priming,in
R4224 T5263 T5266 arg1Of priming,had
R4225 T5265 T5264 arg2Of culture,in
R4226 T5266 T5261 arg2Of had,and
R4227 T5266 T5271 arg1Of had,on
R4228 T5270 T5266 arg2Of effect,had
R4229 T5270 T5267 arg1Of effect,only
R4230 T5270 T5268 arg1Of effect,a
R4231 T5270 T5269 arg1Of effect,modest
R4232 T5273 T5271 arg2Of expression,on
R4233 T5273 T5272 arg1Of expression,its
R4234 T5273 T5274 arg1Of expression,at
R4235 T5275 T5274 arg2Of day,at
R4236 T5275 T5276 arg1Of day,2
R4237 T5275 T5277 arg1Of day,(
R4238 T5278 T5277 arg2Of Fig.,(
R4239 T5278 T5279 arg1Of Fig.,1
R4240 T5278 T5280 arg1Of Fig.,","
R4241 T5281 T5282 arg1Of A,and
R4242 T5282 T5280 arg2Of and,","
R4243 T5283 T5282 arg2Of C,and
R4244 T5284 T5277 arg3Of ),(
R4245 T5286 T5285 arg1Of induction,Strong
R4246 T5286 T5287 arg1Of induction,of
R4247 T5286 T5292 arg1Of induction,was
R4248 T5286 T5294 arg2Of induction,observed
R4249 T5289 T5290 arg1Of mRNA,and
R4250 T5290 T5287 arg2Of and,of
R4251 T5290 T5288 arg1Of and,Eomes
R4252 T5291 T5290 arg2Of protein,and
R4253 T5294 T5292 arg2Of observed,was
R4254 T5294 T5293 arg1Of observed,only
R4255 T5294 T5295 arg1Of observed,at
R4256 T5294 T5300 arg1Of observed,(
R4257 T5296 T5297 arg1Of day,4
R4258 T5296 T5298 arg1Of day,and
R4259 T5299 T5295 arg2Of later,at
R4260 T5299 T5296 arg1Of later,day
R4261 T5301 T5300 arg2Of Fig.,(
R4262 T5301 T5302 arg1Of Fig.,1
R4263 T5301 T5303 arg1Of Fig.,","
R4264 T5304 T5305 arg1Of A,and
R4265 T5305 T5303 arg2Of and,","
R4266 T5306 T5305 arg2Of C,and
R4267 T5307 T5300 arg3Of ),(
R4268 T5310 T5308 arg1Of expression,T-bet
R4269 T5310 T5309 arg1Of expression,mRNA
R4270 T5310 T5312 arg1Of expression,preceded
R4271 T5312 T5311 arg1Of preceded,slightly
R4272 T5312 T5318 arg1Of preceded,;
R4273 T5314 T5312 arg2Of expression,preceded
R4274 T5314 T5313 arg1Of expression,the
R4275 T5314 T5315 arg1Of expression,of
R4276 T5317 T5315 arg2Of mRNA,of
R4277 T5317 T5316 arg1Of mRNA,GzmB
R4278 T5322 T5323 arg1Of mRNA,and
R4279 T5323 T5321 arg1Of and,Eomes
R4280 T5323 T5325 arg1Of and,were
R4281 T5323 T5326 arg2Of and,expressed
R4282 T5324 T5323 arg2Of protein,and
R4283 T5326 T5318 arg2Of expressed,;
R4284 T5326 T5319 arg1Of expressed,similarly
R4285 T5326 T5320 arg1Of expressed,","
R4286 T5326 T5325 arg2Of expressed,were
R4287 T5326 T5327 arg1Of expressed,∼1
R4288 T5326 T5340 arg1Of expressed,(
R4289 T5327 T5328 arg1Of ∼1,d
R4290 T5327 T5329 arg1Of ∼1,ahead
R4291 T5327 T5338 arg1Of ∼1,","
R4292 T5327 T5339 arg1Of ∼1,respectively
R4293 T5329 T5330 arg1Of ahead,of
R4294 T5332 T5330 arg2Of reexpression,of
R4295 T5332 T5331 arg1Of reexpression,the
R4296 T5332 T5333 arg1Of reexpression,of
R4297 T5335 T5334 arg1Of mRNA,perforin
R4298 T5335 T5336 arg1Of mRNA,and
R4299 T5336 T5333 arg2Of and,of
R4300 T5337 T5336 arg2Of protein,and
R4301 T5341 T5342 arg1Of Fig.,1
R4302 T5341 T5343 arg1Of Fig.,","
R4303 T5343 T5345 arg1Of ",",and
R4304 T5344 T5343 arg2Of B,","
R4305 T5345 T5340 arg2Of and,(
R4306 T5346 T5345 arg2Of D,and
R4307 T5347 T5340 arg3Of ),(
R4308 T5351 T5348 arg1Of analysis,This
R4309 T5351 T5349 arg1Of analysis,detailed
R4310 T5351 T5350 arg1Of analysis,kinetic
R4311 T5351 T5352 arg1Of analysis,suggested
R4312 T5358 T5355 arg2Of conditions,under
R4313 T5358 T5356 arg1Of conditions,our
R4314 T5358 T5357 arg1Of conditions,culture
R4315 T5360 T5361 arg1Of T-bet,and
R4316 T5361 T5363 arg1Of and,contribute
R4317 T5362 T5361 arg2Of Eomes,and
R4318 T5363 T5352 arg2Of contribute,suggested
R4319 T5363 T5353 arg1Of contribute,that
R4320 T5363 T5354 arg1Of contribute,","
R4321 T5363 T5355 arg1Of contribute,under
R4322 T5363 T5359 arg1Of contribute,","
R4323 T5363 T5364 arg1Of contribute,to
R4324 T5363 T5370 arg1Of contribute,during
R4325 T5366 T5364 arg2Of aspects,to
R4326 T5366 T5365 arg1Of aspects,distinct
R4327 T5366 T5367 arg1Of aspects,of
R4328 T5369 T5367 arg2Of transcription,of
R4329 T5369 T5368 arg1Of transcription,gene
R4330 T5372 T5370 arg2Of differentiation,during
R4331 T5372 T5371 arg1Of differentiation,CTL
R4332 T5373 T5374 arg1Of T-bet,is
R4333 T5373 T5375 arg2Of T-bet,required
R4334 T5375 T5374 arg2Of required,is
R4335 T5375 T5376 arg1Of required,early
R4336 T5375 T5377 arg1Of required,for
R4337 T5375 T5381 arg1Of required,and
R4338 T5379 T5377 arg2Of production,for
R4339 T5379 T5378 arg1Of production,IFN-γ
R4340 T5381 T5380 arg1Of and,","
R4341 T5383 T5382 arg1Of data,our
R4342 T5383 T5384 arg1Of data,suggested
R4343 T5384 T5381 arg2Of suggested,and
R4344 T5386 T5387 arg1Of Eomes,might
R4345 T5386 T5389 arg1Of Eomes,function
R4346 T5386 T5396 arg1Of Eomes,might
R4347 T5386 T5397 arg1Of Eomes,contribute
R4348 T5389 T5387 arg2Of function,might
R4349 T5389 T5388 arg1Of function,not
R4350 T5389 T5390 arg1Of function,during
R4351 T5389 T5394 arg1Of function,but
R4352 T5393 T5390 arg2Of period,during
R4353 T5393 T5391 arg1Of period,this
R4354 T5393 T5392 arg1Of period,early
R4355 T5394 T5384 arg2Of but,suggested
R4356 T5394 T5385 arg1Of but,that
R4357 T5397 T5394 arg2Of contribute,but
R4358 T5397 T5395 arg1Of contribute,rather
R4359 T5397 T5396 arg2Of contribute,might
R4360 T5397 T5399 arg1Of contribute,to
R4361 T5399 T5398 arg1Of to,later
R4362 T5401 T5399 arg2Of control,to
R4363 T5401 T5400 arg1Of control,the
R4364 T5401 T5402 arg1Of control,of
R4365 T5404 T5402 arg2Of expression,of
R4366 T5404 T5403 arg1Of expression,perforin
R4367 T5406 T5405 arg1Of data,Our
R4368 T5406 T5407 arg1Of data,seemed
R4369 T5406 T5409 arg1Of data,consistent
R4370 T5407 T5432 arg1Of seemed,;
R4371 T5409 T5407 arg2Of consistent,seemed
R4372 T5409 T5408 arg1Of consistent,most
R4373 T5409 T5410 arg1Of consistent,with
R4374 T5412 T5410 arg2Of model,with
R4375 T5412 T5411 arg1Of model,a
R4376 T5412 T5413 arg2Of model,in
R4377 T5412 T5414 arg1Of model,which
R4378 T5412 T5419 arg1Of model,","
R4379 T5412 T5420 arg1Of model,which
R4380 T5412 T5423 arg1Of model,induces
R4381 T5416 T5415 arg1Of signals,TCR
R4382 T5416 T5417 arg1Of signals,induce
R4383 T5417 T5413 arg1Of induce,in
R4384 T5418 T5417 arg2Of T-bet,induce
R4385 T5422 T5421 arg2Of turn,in
R4386 T5423 T5421 arg1Of induces,in
R4387 T5424 T5425 arg1Of IFN-γ,(
R4388 T5424 T5428 arg1Of IFN-γ,and
R4389 T5426 T5425 arg2Of 17,(
R4390 T5427 T5425 arg3Of ),(
R4391 T5428 T5423 arg2Of and,induces
R4392 T5431 T5428 arg2Of B,and
R4393 T5431 T5429 arg1Of B,possibly
R4394 T5431 T5430 arg1Of B,granzyme
R4395 T5435 T5436 arg1Of Eomes,is
R4396 T5435 T5437 arg2Of Eomes,induced
R4397 T5435 T5447 arg1Of Eomes,activates
R4398 T5437 T5436 arg2Of induced,is
R4399 T5437 T5438 arg1Of induced,during
R4400 T5437 T5446 arg1Of induced,and
R4401 T5440 T5438 arg2Of period,during
R4402 T5440 T5439 arg1Of period,the
R4403 T5440 T5441 arg1Of period,of
R4404 T5443 T5441 arg2Of expansion,of
R4405 T5443 T5442 arg1Of expansion,clonal
R4406 T5443 T5444 arg1Of expansion,in
R4407 T5445 T5444 arg2Of IL-2,in
R4408 T5446 T5432 arg2Of and,;
R4409 T5446 T5433 arg1Of and,subsequently
R4410 T5446 T5434 arg1Of and,","
R4411 T5447 T5446 arg2Of activates,and
R4412 T5449 T5447 arg2Of expression,activates
R4413 T5449 T5448 arg1Of expression,perforin
R5177 T6493 T6494 arg1Of Perforin,and
R5178 T6494 T6498 arg1Of and,are
R5179 T6494 T6501 arg2Of and,regulated
R5180 T6497 T6494 arg2Of expression,and
R5181 T6497 T6495 arg1Of expression,granzyme
R5182 T6497 T6496 arg1Of expression,B
R5183 T6501 T6498 arg2Of regulated,are
R5184 T6501 T6499 arg1Of regulated,not
R5185 T6501 T6500 arg1Of regulated,appreciably
R5186 T6503 T6501 arg1Of T-bet,regulated
R5187 T6503 T6502 arg2Of T-bet,by
R5188 T6505 T6504 arg1Of test,To
R5189 T6507 T6505 arg2Of model,test
R5190 T6507 T6506 arg1Of model,the
R5191 T6507 T6508 arg2Of model,outlined
R5192 T6508 T6509 arg1Of outlined,in
R5193 T6508 T6513 arg1Of outlined,directly
R5194 T6512 T6509 arg2Of paragraph,in
R5195 T6512 T6510 arg1Of paragraph,the
R5196 T6512 T6511 arg1Of paragraph,previous
R5197 T6515 T6505 arg1Of we,test
R5198 T6515 T6516 arg1Of we,compared
R5199 T6516 T6504 modOf compared,To
R5200 T6516 T6514 arg1Of compared,","
R5201 T6518 T6516 arg2Of expression,compared
R5202 T6518 T6517 arg1Of expression,the
R5203 T6518 T6519 arg1Of expression,of
R5204 T6518 T6527 arg1Of expression,in
R5205 T6520 T6521 arg1Of IFN-γ,","
R5206 T6521 T6524 arg1Of ",",and
R5207 T6522 T6521 arg2Of perforin,","
R5208 T6524 T6519 arg2Of and,of
R5209 T6524 T6523 arg1Of and,","
R5210 T6526 T6524 arg2Of B,and
R5211 T6526 T6525 arg1Of B,granzyme
R5212 T6530 T6527 arg2Of cells,in
R5213 T6530 T6528 arg1Of cells,CD8+
R5214 T6530 T6529 arg1Of cells,T
R5215 T6530 T6531 arg1Of cells,from
R5216 T6532 T6533 arg1Of WT,and
R5217 T6534 T6533 arg2Of Tbx21,and
R5218 T6536 T6535 arg2Of T-bet,(
R5219 T6537 T6535 arg3Of ),(
R5220 T6539 T6531 arg2Of mice,from
R5221 T6539 T6532 arg1Of mice,WT
R5222 T6539 T6534 arg1Of mice,Tbx21
R5223 T6539 T6535 arg1Of mice,(
R5224 T6539 T6538 arg1Of mice,-deficient
R5225 T6541 T6540 arg2Of expected,As
R5226 T6541 T6542 arg1Of expected,(
R5227 T6543 T6544 arg1Of 17,","
R5228 T6544 T6542 arg2Of ",",(
R5229 T6545 T6544 arg2Of 21,","
R5230 T6546 T6542 arg3Of ),(
R5231 T6552 T6548 arg1Of cells,naive
R5232 T6552 T6549 arg1Of cells,Tbx21−/−
R5233 T6552 T6550 arg1Of cells,CD8+
R5234 T6552 T6551 arg1Of cells,T
R5235 T6552 T6553 arg1Of cells,produced
R5236 T6553 T6540 arg1Of produced,As
R5237 T6553 T6547 arg1Of produced,","
R5238 T6553 T6555 arg1Of produced,poorly
R5239 T6553 T6556 arg1Of produced,upon
R5240 T6554 T6553 arg2Of IFN-γ,produced
R5241 T6557 T6556 arg2Of activation,upon
R5242 T6557 T6558 arg1Of activation,(
R5243 T6561 T6558 arg2Of A,(
R5244 T6561 T6559 arg1Of A,Fig.
R5245 T6561 T6560 arg1Of A,2
R5246 T6562 T6558 arg3Of ),(
R5247 T6567 T6565 arg1Of effect,this
R5248 T6567 T6566 arg1Of effect,deleterious
R5249 T6567 T6568 arg1Of effect,of
R5250 T6567 T6571 arg1Of effect,was
R5251 T6567 T6573 arg2Of effect,observed
R5252 T6567 T6585 arg1Of effect,was
R5253 T6567 T6588 arg2Of effect,mitigated
R5254 T6570 T6568 arg2Of deficiency,of
R5255 T6570 T6569 arg1Of deficiency,T-bet
R5256 T6573 T6571 arg2Of observed,was
R5257 T6573 T6572 arg1Of observed,only
R5258 T6573 T6574 arg1Of observed,in
R5259 T6573 T6579 arg1Of observed,until
R5260 T6573 T6584 arg1Of observed,but
R5261 T6578 T6574 arg2Of cells,in
R5262 T6578 T6575 arg1Of cells,differentiating
R5263 T6578 T6576 arg1Of cells,CD8+
R5264 T6578 T6577 arg1Of cells,T
R5265 T6580 T6579 arg2Of day,until
R5266 T6580 T6581 arg1Of day,4
R5267 T6580 T6582 arg1Of day,of
R5268 T6583 T6582 arg2Of culture,of
R5269 T6584 T6563 arg1Of but,Notably
R5270 T6584 T6564 arg1Of but,","
R5271 T6587 T6586 arg1Of completely,almost
R5272 T6588 T6584 arg2Of mitigated,but
R5273 T6588 T6585 arg2Of mitigated,was
R5274 T6588 T6587 arg1Of mitigated,completely
R5275 T6588 T6589 arg1Of mitigated,by
R5276 T6590 T6589 arg2Of day,by
R5277 T6590 T6591 arg1Of day,6
R5278 T6590 T6592 arg1Of day,(
R5279 T6595 T6592 arg2Of A,(
R5280 T6595 T6593 arg1Of A,Fig.
R5281 T6595 T6594 arg1Of A,2
R5282 T6596 T6592 arg3Of ),(
R5283 T6599 T6598 arg1Of likely,most
R5284 T6601 T6597 arg1Of compensation,This
R5285 T6601 T6599 arg1Of compensation,likely
R5286 T6601 T6600 arg2Of compensation,reflected
R5287 T6601 T6602 arg1Of compensation,by
R5288 T6601 T6604 arg1Of compensation,","
R5289 T6601 T6605 arg1Of compensation,which
R5290 T6601 T6606 arg1Of compensation,was
R5291 T6601 T6608 arg2Of compensation,induced
R5292 T6603 T6602 arg2Of Eomes,by
R5293 T6608 T6606 arg2Of induced,was
R5294 T6608 T6607 arg1Of induced,strongly
R5295 T6608 T6609 arg1Of induced,between
R5296 T6611 T6612 arg1Of 4,and
R5297 T6612 T6609 arg2Of and,between
R5298 T6612 T6610 arg1Of and,days
R5299 T6612 T6614 arg1Of and,(
R5300 T6613 T6612 arg2Of 6,and
R5301 T6615 T6614 arg2Of Fig.,(
R5302 T6615 T6616 arg1Of Fig.,1
R5303 T6617 T6614 arg3Of ),(
R5304 T6619 T6618 arg2Of contrast,In
R5305 T6623 T6621 arg1Of cells,T-bet–deficient
R5306 T6623 T6622 arg1Of cells,T
R5307 T6623 T6624 arg2Of cells,cultured
R5308 T6623 T6628 arg1Of cells,showed
R5309 T6624 T6625 arg1Of cultured,for
R5310 T6627 T6625 arg2Of d,for
R5311 T6627 T6626 arg1Of d,6
R5312 T6628 T6618 arg1Of showed,In
R5313 T6628 T6620 arg1Of showed,","
R5314 T6628 T6631 arg1Of showed,in
R5315 T6630 T6628 arg2Of defect,showed
R5316 T6630 T6629 arg1Of defect,no
R5317 T6634 T6631 arg2Of expression,in
R5318 T6634 T6632 arg1Of expression,perforin
R5319 T6634 T6633 arg1Of expression,mRNA
R5320 T6634 T6635 arg1Of expression,(
R5321 T6638 T6636 arg1Of B,Fig.
R5322 T6638 T6637 arg1Of B,2
R5323 T6638 T6640 arg1Of B,compare
R5324 T6640 T6635 arg2Of compare,(
R5325 T6640 T6639 arg1Of compare,","
R5326 T6642 T6643 arg1Of 1,and
R5327 T6643 T6640 arg2Of and,compare
R5328 T6643 T6641 arg1Of and,lanes
R5329 T6644 T6643 arg2Of 4,and
R5330 T6645 T6635 arg3Of ),(
R5331 T6646 T6648 arg1Of We,observed
R5332 T6648 T6647 arg1Of observed,consistently
R5333 T6648 T6659 arg1Of observed,(
R5334 T6648 T6670 arg1Of observed,","
R5335 T6648 T6671 arg1Of observed,which
R5336 T6648 T6672 arg1Of observed,did
R5337 T6648 T6674 arg1Of observed,translate
R5338 T6651 T6648 arg2Of reduction,observed
R5339 T6651 T6649 arg1Of reduction,a
R5340 T6651 T6650 arg1Of reduction,modest
R5341 T6651 T6652 arg1Of reduction,in
R5342 T6651 T6655 arg1Of reduction,in
R5343 T6654 T6652 arg2Of mRNA,in
R5344 T6654 T6653 arg1Of mRNA,GzmB
R5345 T6658 T6655 arg2Of cells,in
R5346 T6658 T6656 arg1Of cells,T-bet–deficient
R5347 T6658 T6657 arg1Of cells,T
R5348 T6662 T6660 arg1Of B,Fig.
R5349 T6662 T6661 arg1Of B,2
R5350 T6662 T6664 arg1Of B,compare
R5351 T6664 T6659 arg2Of compare,(
R5352 T6664 T6663 arg1Of compare,","
R5353 T6666 T6667 arg1Of 1,and
R5354 T6667 T6664 arg2Of and,compare
R5355 T6667 T6665 arg1Of and,lanes
R5356 T6668 T6667 arg2Of 4,and
R5357 T6669 T6659 arg3Of ),(
R5358 T6674 T6672 arg2Of translate,did
R5359 T6674 T6673 arg1Of translate,not
R5360 T6674 T6675 arg1Of translate,into
R5361 T6677 T6675 arg2Of decrease,into
R5362 T6677 T6676 arg1Of decrease,a
R5363 T6677 T6678 arg1Of decrease,in
R5364 T6679 T6678 arg2Of expression,in
R5365 T6679 T6680 arg1Of expression,of
R5366 T6683 T6680 arg2Of protein,of
R5367 T6683 T6681 arg1Of protein,granzyme
R5368 T6683 T6682 arg1Of protein,B
R5369 T6683 T6684 arg1Of protein,(
R5370 T6687 T6684 arg2Of C,(
R5371 T6687 T6685 arg1Of C,Fig.
R5372 T6687 T6686 arg1Of C,2
R5373 T6688 T6684 arg3Of ),(
R5374 T6690 T6689 arg1Of examine,To
R5375 T6692 T6690 arg2Of role,examine
R5376 T6692 T6691 arg1Of role,the
R5377 T6692 T6693 arg1Of role,of
R5378 T6694 T6693 arg2Of Eomes,of
R5379 T6696 T6697 arg1Of we,transduced
R5380 T6697 T6689 modOf transduced,To
R5381 T6697 T6695 arg1Of transduced,","
R5382 T6701 T6697 arg2Of cells,transduced
R5383 T6701 T6698 arg1Of cells,naive
R5384 T6701 T6699 arg1Of cells,CD8+
R5385 T6701 T6700 arg1Of cells,T
R5386 T6701 T6702 arg1Of cells,from
R5387 T6701 T6707 arg1Of cells,with
R5388 T6703 T6704 arg1Of WT,and
R5389 T6705 T6704 arg2Of Tbx21−/−,and
R5390 T6706 T6702 arg2Of mice,from
R5391 T6706 T6703 arg1Of mice,WT
R5392 T6706 T6705 arg1Of mice,Tbx21−/−
R5393 T6708 T6707 arg2Of retroviruses,with
R5394 T6708 T6709 arg1Of retroviruses,containing
R5395 T6713 T6710 arg1Of site,internal
R5396 T6713 T6711 arg1Of site,ribosome
R5397 T6713 T6712 arg1Of site,entry
R5398 T6713 T6714 arg1Of site,(
R5399 T6715 T6714 arg2Of IRES,(
R5400 T6716 T6714 arg3Of ),(
R5401 T6717 T6709 arg2Of –GFP,containing
R5402 T6717 T6713 arg1Of –GFP,site
R5403 T6717 T6718 arg1Of –GFP,that
R5404 T6717 T6719 arg1Of –GFP,were
R5405 T6717 T6721 arg1Of –GFP,empty
R5406 T6717 T6723 arg1Of –GFP,encoded
R5407 T6717 T6738 arg1Of –GFP,expanded
R5408 T6719 T6722 arg1Of were,or
R5409 T6721 T6719 arg2Of empty,were
R5410 T6721 T6720 arg1Of empty,either
R5411 T6722 T6737 arg1Of or,and
R5412 T6723 T6722 arg2Of encoded,or
R5413 T6726 T6725 arg1Of transactivating,strongly
R5414 T6727 T6723 arg2Of version,encoded
R5415 T6727 T6724 arg1Of version,a
R5416 T6727 T6726 arg1Of version,transactivating
R5417 T6727 T6728 arg1Of version,of
R5418 T6729 T6728 arg2Of Eomes,of
R5419 T6729 T6730 arg1Of Eomes,(
R5420 T6729 T6733 arg1Of Eomes,(
R5421 T6731 T6730 arg2Of Eo-VP16,(
R5422 T6732 T6730 arg3Of ),(
R5423 T6734 T6733 arg2Of 8,(
R5424 T6735 T6733 arg3Of ),(
R5425 T6737 T6736 arg1Of and,","
R5426 T6738 T6737 arg2Of expanded,and
R5427 T6738 T6740 arg1Of expanded,for
R5428 T6738 T6743 arg1Of expanded,under
R5429 T6739 T6738 arg2Of them,expanded
R5430 T6742 T6740 arg2Of d,for
R5431 T6742 T6741 arg1Of d,6
R5432 T6746 T6743 arg2Of conditions,under
R5433 T6746 T6744 arg1Of conditions,our
R5434 T6746 T6745 arg1Of conditions,culture
R5435 T6747 T6749 arg1Of Eo-VP16,but
R5436 T6749 T6748 arg1Of but,","
R5437 T6749 T6750 arg1Of but,not
R5438 T6749 T6756 arg1Of but,increased
R5439 T6754 T6749 arg2Of retrovirus,but
R5440 T6754 T6751 arg1Of retrovirus,the
R5441 T6754 T6752 arg1Of retrovirus,empty
R5442 T6754 T6753 arg1Of retrovirus,GFP
R5443 T6756 T6755 arg1Of increased,","
R5444 T6758 T6756 arg2Of expression,increased
R5445 T6758 T6757 arg1Of expression,perforin
R5446 T6758 T6759 arg1Of expression,in
R5447 T6766 T6759 arg2Of cells,in
R5448 T6766 T6760 arg1Of cells,both
R5449 T6766 T6761 arg1Of cells,WT
R5450 T6766 T6762 arg1Of cells,and
R5451 T6766 T6763 arg1Of cells,T-bet–deficient
R5452 T6766 T6764 arg1Of cells,CD8+
R5453 T6766 T6765 arg1Of cells,T
R5454 T6766 T6767 arg1Of cells,(
R5455 T6770 T6767 arg2Of B,(
R5456 T6770 T6768 arg1Of B,Fig.
R5457 T6770 T6769 arg1Of B,2
R5458 T6770 T6771 arg1Of B,","
R5459 T6773 T6774 arg1Of 2,","
R5460 T6774 T6776 arg1Of ",",","
R5461 T6775 T6774 arg2Of 3,","
R5462 T6776 T6772 arg1Of ",",lanes
R5463 T6776 T6779 arg1Of ",",and
R5464 T6777 T6776 arg2Of 5,","
R5465 T6779 T6771 arg2Of and,","
R5466 T6779 T6778 arg1Of and,","
R5467 T6780 T6779 arg2Of 6,and
R5468 T6781 T6767 arg3Of ),(
R5469 T6783 T6782 arg2Of expected,As
R5470 T6785 T6787 arg1Of Eo-VP16,rescued
R5471 T6787 T6782 arg1Of rescued,As
R5472 T6787 T6784 arg1Of rescued,","
R5473 T6787 T6786 arg1Of rescued,also
R5474 T6790 T6787 arg2Of defect,rescued
R5475 T6790 T6788 arg1Of defect,the
R5476 T6790 T6789 arg1Of defect,early
R5477 T6790 T6791 arg1Of defect,in
R5478 T6793 T6791 arg2Of production,in
R5479 T6793 T6792 arg1Of production,IFN-γ
R5480 T6793 T6794 arg2Of production,observed
R5481 T6794 T6795 arg1Of observed,in
R5482 T6799 T6795 arg2Of cells,in
R5483 T6799 T6796 arg1Of cells,T-bet–deficient
R5484 T6799 T6797 arg1Of cells,CD8+
R5485 T6799 T6798 arg1Of cells,T
R5486 T6799 T6800 arg1Of cells,(
R5487 T6803 T6800 arg2Of D,(
R5488 T6803 T6801 arg1Of D,Fig.
R5489 T6803 T6802 arg1Of D,2
R5490 T6804 T6800 arg3Of ),(
R5491 T6807 T6808 arg1Of Eo-VP16,did
R5492 T6807 T6810 arg1Of Eo-VP16,induce
R5493 T6810 T6805 arg1Of induce,However
R5494 T6810 T6806 arg1Of induce,","
R5495 T6810 T6808 arg2Of induce,did
R5496 T6810 T6809 arg1Of induce,not
R5497 T6810 T6820 arg1Of induce,;
R5498 T6813 T6810 arg2Of expression,induce
R5499 T6813 T6811 arg1Of expression,GzmB
R5500 T6813 T6812 arg1Of expression,mRNA
R5501 T6813 T6814 arg1Of expression,in
R5502 T6816 T6817 arg1Of WT,or
R5503 T6817 T6814 arg2Of or,in
R5504 T6817 T6815 arg1Of or,either
R5505 T6819 T6817 arg2Of cells,or
R5506 T6819 T6818 arg1Of cells,T-bet–deficient
R5507 T6826 T6823 arg1Of dependence,the
R5508 T6826 T6824 arg1Of dependence,partial
R5509 T6826 T6825 arg1Of dependence,T-bet
R5510 T6826 T6827 arg1Of dependence,of
R5511 T6826 T6831 arg1Of dependence,can
R5512 T6826 T6833 arg1Of dependence,be
R5513 T6826 T6834 arg2Of dependence,compensated
R5514 T6830 T6827 arg2Of expression,of
R5515 T6830 T6828 arg1Of expression,GzmB
R5516 T6830 T6829 arg1Of expression,mRNA
R5517 T6834 T6820 arg2Of compensated,;
R5518 T6834 T6821 arg1Of compensated,thus
R5519 T6834 T6822 arg1Of compensated,","
R5520 T6834 T6831 arg2Of compensated,can
R5521 T6834 T6832 arg1Of compensated,not
R5522 T6834 T6833 arg2Of compensated,be
R5523 T6834 T6835 arg1Of compensated,for
R5524 T6837 T6834 arg1Of Eo-VP16,compensated
R5525 T6837 T6836 arg2Of Eo-VP16,by
R7154 T8977 T8978 arg1Of Runx3,controls
R7155 T8980 T8978 arg2Of aspects,controls
R7156 T8980 T8979 arg1Of aspects,multiple
R7157 T8980 T8981 arg1Of aspects,of
R7158 T8980 T8986 arg1Of aspects,","
R7159 T8980 T8989 arg1Of aspects,through
R7160 T8985 T8981 arg2Of program,of
R7161 T8985 T8982 arg1Of program,the
R7162 T8985 T8983 arg1Of program,CTL
R7163 T8985 T8984 arg1Of program,differentiation
R7164 T8988 T8987 arg2Of part,in
R7165 T8989 T8987 arg1Of through,in
R7166 T8990 T8989 arg2Of induction,through
R7167 T8990 T8991 arg1Of induction,of
R7168 T8992 T8991 arg2Of Eomes,of
R7169 T8994 T8995 arg1Of Runx3,is
R7170 T8994 T8997 arg2Of Runx3,expressed
R7171 T8997 T8993 arg2Of expressed,Because
R7172 T8997 T8995 arg2Of expressed,is
R7173 T8997 T8996 arg1Of expressed,highly
R7174 T8997 T8998 arg1Of expressed,in
R7175 T8997 T9005 arg1Of expressed,because
R7176 T8998 T9004 arg1Of in,and
R7177 T9002 T8998 arg2Of cells,in
R7178 T9002 T8999 arg1Of cells,peripheral
R7179 T9002 T9000 arg1Of cells,CD8+
R7180 T9002 T9001 arg1Of cells,T
R7181 T9004 T9003 arg1Of and,","
R7182 T9005 T9004 arg2Of because,and
R7183 T9005 T9006 arg1Of because,of
R7184 T9009 T9005 arg2Of cooperation,because
R7185 T9009 T9007 arg1Of cooperation,the
R7186 T9009 T9008 arg1Of cooperation,T-bet-Runx3
R7187 T9009 T9011 arg2Of cooperation,observed
R7188 T9010 T9011 arg1Of we,observed
R7189 T9011 T9012 arg1Of observed,earlier
R7190 T9012 T9013 arg1Of earlier,in
R7191 T9016 T9013 arg2Of cells,in
R7192 T9016 T9014 arg1Of cells,CD4+
R7193 T9016 T9015 arg1Of cells,T
R7194 T9016 T9017 arg1Of cells,(
R7195 T9018 T9017 arg2Of 15,(
R7196 T9019 T9017 arg3Of ),(
R7197 T9021 T9022 arg1Of we,examined
R7198 T9022 T8993 arg1Of examined,Because
R7199 T9022 T9020 arg1Of examined,","
R7200 T9024 T9022 arg2Of role,examined
R7201 T9024 T9023 arg1Of role,the
R7202 T9024 T9025 arg1Of role,of
R7203 T9024 T9027 arg1Of role,in
R7204 T9026 T9025 arg2Of Runx3,of
R7205 T9030 T9027 arg2Of differentiation,in
R7206 T9030 T9028 arg1Of differentiation,effector
R7207 T9030 T9029 arg1Of differentiation,CTL
R7208 T9031 T9032 arg1Of We,isolated
R7209 T9032 T9052 arg1Of isolated,by
R7210 T9035 T9033 arg1Of cells,CD8+
R7211 T9035 T9034 arg1Of cells,T
R7212 T9035 T9036 arg1Of cells,from
R7213 T9035 T9047 arg1Of cells,and
R7214 T9039 T9038 arg2Of KO,(
R7215 T9040 T9038 arg3Of ),(
R7216 T9041 T9036 arg2Of mice,from
R7217 T9041 T9037 arg1Of mice,Runx3−/−
R7218 T9041 T9038 arg1Of mice,(
R7219 T9041 T9042 arg1Of mice,of
R7220 T9046 T9042 arg2Of background,of
R7221 T9046 T9043 arg1Of background,the
R7222 T9046 T9044 arg1Of background,outbred
R7223 T9046 T9045 arg1Of background,ICR
R7224 T9047 T9032 arg2Of and,isolated
R7225 T9051 T9047 arg2Of littermates,and
R7226 T9051 T9048 arg1Of littermates,their
R7227 T9051 T9049 arg1Of littermates,WT
R7228 T9051 T9050 arg1Of littermates,Runx3+/+
R7229 T9054 T9052 arg2Of selection,by
R7230 T9054 T9053 arg1Of selection,positive
R7231 T9054 T9055 arg1Of selection,with
R7232 T9058 T9055 arg2Of beads,with
R7233 T9058 T9056 arg1Of beads,anti-CD8
R7234 T9058 T9057 arg1Of beads,magnetic
R7235 T9058 T9059 arg1Of beads,(
R7236 T9061 T9062 arg1Of S1,and
R7237 T9062 T9059 arg2Of and,(
R7238 T9062 T9060 arg1Of and,Figs.
R7239 T9062 T9064 arg1Of and,","
R7240 T9062 T9065 arg1Of and,available
R7241 T9062 T9068 arg1Of and,:
R7242 T9063 T9062 arg2Of S2,and
R7243 T9065 T9066 arg1Of available,at
R7244 T9067 T9066 arg2Of http,at
R7245 T9069 T9068 arg2Of //www.jem.org/cgi/content/full/jem.20081242/DC1,:
R7246 T9070 T9059 arg3Of ),(
R7247 T9076 T9073 arg1Of cells,Runx3−/−
R7248 T9076 T9074 arg1Of cells,CD8+
R7249 T9076 T9075 arg1Of cells,T
R7250 T9076 T9077 arg1Of cells,were
R7251 T9076 T9079 arg1Of cells,impaired
R7252 T9077 T9071 arg1Of were,Strikingly
R7253 T9077 T9072 arg1Of were,","
R7254 T9077 T9088 arg1Of were,","
R7255 T9079 T9077 arg2Of impaired,were
R7256 T9079 T9078 arg1Of impaired,strongly
R7257 T9079 T9080 arg1Of impaired,in
R7258 T9082 T9080 arg2Of ability,in
R7259 T9082 T9081 arg1Of ability,their
R7260 T9082 T9083 modOf ability,to
R7261 T9084 T9083 arg1Of differentiate,to
R7262 T9084 T9085 arg1Of differentiate,into
R7263 T9087 T9085 arg2Of CTLs,into
R7264 T9087 T9086 arg1Of CTLs,effector
R7265 T9089 T9077 arg3Of as,were
R7266 T9090 T9089 arg2Of judged,as
R7267 T9092 T9090 arg1Of expression,judged
R7268 T9092 T9091 arg2Of expression,by
R7269 T9092 T9093 arg1Of expression,of
R7270 T9094 T9095 arg1Of perforin,","
R7271 T9095 T9099 arg1Of ",",and
R7272 T9097 T9095 arg2Of B,","
R7273 T9097 T9096 arg1Of B,granzyme
R7274 T9099 T9093 arg2Of and,of
R7275 T9099 T9098 arg1Of and,","
R7276 T9100 T9099 arg2Of IFN-γ,and
R7277 T9100 T9101 arg1Of IFN-γ,(
R7278 T9102 T9101 arg2Of Fig.,(
R7279 T9102 T9103 arg1Of Fig.,3
R7280 T9104 T9101 arg3Of ),(
R7281 T9106 T9105 arg2Of with,Compared
R7282 T9109 T9106 arg2Of cells,with
R7283 T9135 T9128 arg3Of ),(
R7284 T9109 T9107 arg1Of cells,WT
R7285 T9109 T9108 arg1Of cells,T
R7286 T9138 T9136 arg1Of cells,Runx3−/−
R7287 T9138 T9137 arg1Of cells,T
R7288 T9138 T9140 arg1Of cells,had
R7289 T9112 T9113 arg1Of mRNA,and
R7290 T9140 T9139 arg1Of had,also
R7291 T9140 T9145 arg1Of had,;
R7292 T9143 T9141 arg1Of Eomes,no
R7293 T9143 T9142 arg1Of Eomes,detectable
R7294 T9144 T9140 arg2Of expression,had
R7295 T9144 T9143 arg1Of expression,Eomes
R7296 T9114 T9113 arg2Of protein,and
R7297 T9147 T9146 arg2Of contrast,in
R7298 T9115 T9111 arg1Of expression,perforin
R7299 T9150 T9149 arg1Of expression,T-bet
R7300 T9150 T9151 arg1Of expression,was
R7301 T9150 T9152 arg1Of expression,unimpaired
R7302 T9151 T9145 arg2Of was,;
R7303 T9151 T9146 arg1Of was,in
R7304 T9151 T9148 arg1Of was,","
R7305 T9152 T9151 arg2Of unimpaired,was
R7306 T9152 T9153 arg1Of unimpaired,(
R7307 T9115 T9112 arg1Of expression,mRNA
R7308 T9115 T9114 arg1Of expression,protein
R7309 T9115 T9116 arg1Of expression,were
R7310 T9115 T9118 arg1Of expression,undetectable
R7311 T9156 T9153 arg2Of A,(
R7312 T9156 T9154 arg1Of A,Fig.
R7313 T9156 T9155 arg1Of A,3
R7314 T9157 T9153 arg3Of ),(
R7315 T9116 T9105 arg1Of were,Compared
R7316 T9160 T9161 arg1Of Runx3,was
R7317 T9160 T9162 arg2Of Runx3,required
R7318 T9162 T9158 arg1Of required,Furthermore
R7319 T9162 T9159 arg1Of required,","
R7320 T9162 T9161 arg2Of required,was
R7321 T9162 T9163 arg1Of required,for
R7322 T9162 T9174 arg1Of required,","
R7323 T9162 T9175 arg1Of required,by
R7324 T9116 T9110 arg1Of were,","
R7325 T9165 T9163 arg2Of production,for
R7326 T9165 T9164 arg1Of production,maximal
R7327 T9165 T9166 arg1Of production,of
R7328 T9118 T9116 arg2Of undetectable,were
R7329 T9167 T9169 arg1Of IFN-γ,but
R7330 T9169 T9166 arg2Of but,of
R7331 T9169 T9168 arg1Of but,","
R7332 T9169 T9170 arg1Of but,not
R7333 T9171 T9172 arg1Of TNF,or
R7334 T9118 T9117 arg1Of undetectable,essentially
R7335 T9172 T9169 arg2Of or,but
R7336 T9173 T9172 arg2Of IL-2,or
R7337 T9118 T9119 arg1Of undetectable,in
R7338 T9122 T9119 arg2Of cells,in
R7339 T9178 T9175 arg2Of cells,by
R7340 T9178 T9176 arg1Of cells,CD8+
R7341 T9178 T9177 arg1Of cells,T
R7342 T9178 T9179 arg2Of cells,restimulated
R7343 T9179 T9180 arg1Of restimulated,at
R7344 T9122 T9120 arg1Of cells,Runx3−/−
R7345 T9181 T9180 arg2Of day,at
R7346 T9181 T9182 arg1Of day,6
R7347 T9181 T9183 arg1Of day,(
R7348 T9122 T9121 arg1Of cells,T
R7349 T9122 T9123 arg1Of cells,at
R7350 T9124 T9123 arg2Of day,at
R7351 T9186 T9183 arg2Of C,(
R7352 T9186 T9184 arg1Of C,Fig.
R7353 T9186 T9185 arg1Of C,3
R7354 T9187 T9183 arg3Of ),(
R7355 T9188 T9190 arg1Of We,reported
R7356 T9124 T9125 arg1Of day,6
R7357 T9124 T9126 arg1Of day,of
R7358 T9127 T9126 arg2Of culture,of
R7359 T9188 T9208 arg1Of We,induces
R7360 T9188 T9225 arg1Of We,promoting
R7361 T9190 T9207 arg1Of reported,and
R7362 T9127 T9128 arg1Of culture,(
R7363 T9129 T9128 arg2Of Fig.,(
R7364 T9194 T9192 arg1Of differentiation,Th1
R7365 T9194 T9193 arg1Of differentiation,cell
R7366 T9194 T9195 arg1Of differentiation,was
R7367 T9194 T9196 arg2Of differentiation,regulated
R7368 T9196 T9190 arg2Of regulated,reported
R7369 T9196 T9191 arg1Of regulated,that
R7370 T9196 T9195 arg2Of regulated,was
R7371 T9196 T9197 arg1Of regulated,through
R7372 T9200 T9197 arg2Of loop,through
R7373 T9200 T9198 arg1Of loop,a
R7374 T9200 T9199 arg1Of loop,feed-forward
R7375 T9200 T9201 arg2Of loop,in
R7376 T9200 T9202 arg1Of loop,which
R7377 T9129 T9130 arg1Of Fig.,3
R7378 T9203 T9204 arg1Of T-bet,is
R7379 T9203 T9205 arg2Of T-bet,up-regulated
R7380 T9129 T9131 arg1Of Fig.,","
R7381 T9132 T9133 arg1Of A,and
R7382 T9205 T9201 arg1Of up-regulated,in
R7383 T9205 T9204 arg2Of up-regulated,is
R7384 T9205 T9206 arg1Of up-regulated,early
R7385 T9207 T9189 arg1Of and,previously
R7386 T9208 T9207 arg2Of induces,and
R7387 T9208 T9223 arg1Of induces,","
R7388 T9208 T9225 modOf induces,promoting
R7389 T9209 T9208 arg2Of Runx3,induces
R7390 T9209 T9210 arg1Of Runx3,","
R7391 T9209 T9211 arg2Of Runx3,after
R7392 T9209 T9212 arg1Of Runx3,which
R7393 T9133 T9131 arg2Of and,","
R7394 T9213 T9214 arg1Of T-bet,and
R7395 T9214 T9216 arg1Of and,cooperate
R7396 T9214 T9218 arg1Of and,induce
R7397 T9134 T9133 arg2Of B,and
R7398 T9215 T9214 arg2Of Runx3,and
R7399 T9216 T9211 arg1Of cooperate,after
R7400 T9233 T9232 arg2Of 15,(
R7401 T9218 T9216 arg2Of induce,cooperate
R7402 T9218 T9217 arg1Of induce,to
R7403 T9219 T9220 arg1Of IFN-γ,and
R7404 T9221 T9220 arg2Of silence,and
R7405 T9222 T9218 arg2Of IL-4,induce
R7406 T9222 T9219 arg1Of IL-4,IFN-γ
R7407 T9222 T9221 arg1Of IL-4,silence
R7408 T9233 T9234 arg1Of 15,","
R7409 T9235 T9234 arg2Of 22,","
R7410 T9225 T9224 arg1Of promoting,thus
R7411 T9227 T9225 arg2Of differentiation,promoting
R7412 T9227 T9226 arg1Of differentiation,stable
R7413 T9227 T9228 arg1Of differentiation,toward
R7414 T9227 T9232 arg1Of differentiation,(
R7415 T9236 T9232 arg3Of ),(
R7416 T9241 T9239 arg1Of Runx3,a
R7417 T9231 T9228 arg2Of lineage,toward
R7418 T9231 T9229 arg1Of lineage,the
R7419 T9231 T9230 arg1Of lineage,Th1
R7420 T9241 T9240 arg1Of Runx3,)
R7421 T9241 T9242 arg1Of Runx3,appeared
R7422 T9241 T9243 arg1Of Runx3,necessary
R7423 T9242 T9252 arg1Of appeared,","
R7424 T9243 T9242 arg2Of necessary,appeared
R7425 T9243 T9244 arg1Of necessary,for
R7426 T9330 T9331 arg1Of we,asked
R7427 T9246 T9244 arg2Of induction,for
R7428 T9331 T9328 arg1Of asked,Specifically
R7429 T9331 T9329 arg1Of asked,","
R7430 T9333 T9334 arg1Of Runx3,","
R7431 T9333 T9335 arg1Of Runx3,which
R7432 T9333 T9336 arg1Of Runx3,was
R7433 T9333 T9337 arg1Of Runx3,necessary
R7434 T9246 T9245 arg1Of induction,Eomes
R7435 T9333 T9343 arg1Of Runx3,cooperated
R7436 T9246 T9247 arg1Of induction,(
R7437 T9333 T9347 arg1Of Runx3,regulate
R7438 T9250 T9247 arg2Of A,(
R7439 T9337 T9336 arg2Of necessary,was
R7440 T9337 T9338 arg1Of necessary,for
R7441 T9250 T9248 arg1Of A,Fig.
R7442 T9340 T9338 arg2Of induction,for
R7443 T9340 T9339 arg1Of induction,Eomes
R7444 T9250 T9249 arg1Of A,3
R7445 T9251 T9247 arg3Of ),(
R7446 T9343 T9331 arg2Of cooperated,asked
R7447 T9343 T9332 arg1Of cooperated,whether
R7448 T9343 T9341 arg1Of cooperated,","
R7449 T9343 T9342 arg1Of cooperated,then
R7450 T9343 T9344 arg1Of cooperated,with
R7451 T9343 T9346 modOf cooperated,to
R7452 T9252 T9237 arg2Of ",",Because
R7453 T9345 T9344 arg2Of Eomes,with
R7454 T9252 T9238 arg1Of ",",(
R7455 T9347 T9346 arg1Of regulate,to
R7456 T9254 T9253 arg2Of b,(
R7457 T9348 T9347 arg2Of transcription,regulate
R7458 T9348 T9349 arg1Of transcription,of
R7459 T9255 T9253 arg3Of ),(
R7460 T9354 T9355 arg1Of perforin,","
R7461 T9355 T9358 arg1Of ",",and
R7462 T9257 T9256 arg1Of kinetics,the
R7463 T9356 T9355 arg2Of IFN-γ,","
R7464 T9358 T9349 arg2Of and,of
R7465 T9358 T9350 arg1Of and,the
R7466 T9358 T9351 arg1Of and,effector
R7467 T9358 T9352 arg1Of and,CTL
R7468 T9358 T9353 arg1Of and,markers
R7469 T9358 T9357 arg1Of and,","
R7470 T9360 T9358 arg2Of B,and
R7471 T9360 T9359 arg1Of B,granzyme
R7472 T9362 T9361 arg1Of test,To
R7473 T9257 T9258 arg1Of kinetics,of
R7474 T9364 T9362 arg2Of hypothesis,test
R7475 T9364 T9363 arg1Of hypothesis,this
R7476 T9366 T9362 arg1Of we,test
R7477 T9366 T9367 arg1Of we,used
R7478 T9366 T9375 arg1Of we,ask
R7479 T9367 T9361 modOf used,To
R7480 T9367 T9365 arg1Of used,","
R7481 T9257 T9261 arg1Of kinetics,paralleled
R7482 T9260 T9258 arg2Of expression,of
R7483 T9371 T9370 arg2Of ChIP,(
R7484 T9372 T9370 arg3Of ),(
R7485 T9373 T9367 arg2Of assays,used
R7486 T9373 T9368 arg1Of assays,chromatin
R7487 T9373 T9369 arg1Of assays,immunoprecipitation
R7488 T9373 T9370 arg1Of assays,(
R7489 T9260 T9259 arg1Of expression,Eomes
R7490 T9375 T9367 arg3Of ask,used
R7491 T9375 T9374 arg1Of ask,to
R7492 T9261 T9271 arg1Of paralleled,and
R7493 T9377 T9378 arg1Of Eomes,and
R7494 T9378 T9380 arg1Of and,bound
R7495 T9379 T9378 arg2Of Runx3,and
R7496 T9380 T9375 arg2Of bound,ask
R7497 T9380 T9376 arg1Of bound,whether
R7498 T9262 T9261 arg2Of those,paralleled
R7499 T9382 T9380 arg2Of regions,bound
R7500 T9382 T9381 arg1Of regions,regulatory
R7501 T9382 T9383 arg1Of regions,of
R7502 T9262 T9263 arg1Of those,of
R7503 T9265 T9263 arg2Of expression,of
R7504 T9385 T9386 arg1Of Prf1,","
R7505 T9386 T9389 arg1Of ",",and
R7506 T9387 T9386 arg2Of Ifng,","
R7507 T9389 T9383 arg2Of and,of
R7508 T9389 T9384 arg1Of and,the
R7509 T9389 T9388 arg1Of and,","
R7510 T9265 T9264 arg1Of expression,perforin
R7511 T9391 T9389 arg2Of genes,and
R7512 T9391 T9390 arg1Of genes,Gzmb
R7513 T9391 T9392 arg1Of genes,(
R7514 T9265 T9266 arg1Of expression,(
R7515 T9267 T9266 arg2Of Fig.,(
R7516 T9395 T9392 arg2Of D,(
R7517 T9395 T9393 arg1Of D,Fig.
R7518 T9395 T9394 arg1Of D,3
R7519 T9396 T9392 arg3Of ),(
R7520 T9398 T9397 arg1Of proteins,Both
R7521 T9398 T9399 arg2Of proteins,associated
R7522 T9399 T9400 arg1Of associated,with
R7523 T9267 T9268 arg1Of Fig.,2
R7524 T9269 T9266 arg3Of ),(
R7525 T9403 T9400 arg2Of regions,with
R7526 T9403 T9401 arg1Of regions,gene
R7527 T9403 T9402 arg1Of regions,regulatory
R7528 T9403 T9404 arg1Of regions,in
R7529 T9406 T9404 arg2Of CTLs,in
R7530 T9406 T9405 arg2Of CTLs,differentiated
R7531 T9407 T9408 arg1Of Runx3,bound
R7532 T9271 T9252 arg2Of and,","
R7533 T9408 T9409 arg1Of bound,to
R7534 T9271 T9253 arg1Of and,(
R7535 T9408 T9420 arg1Of bound,;
R7536 T9408 T9421 arg1Of bound,to
R7537 T9408 T9438 arg1Of bound,;
R7538 T9408 T9439 arg1Of bound,to
R7539 T9408 T9455 arg1Of bound,;
R7540 T9408 T9456 arg1Of bound,to
R7541 T9271 T9270 arg1Of and,","
R7542 T9273 T9272 arg2Of c,(
R7543 T9411 T9412 arg1Of Prf1,and
R7544 T9413 T9412 arg2Of Gzmb,and
R7545 T9274 T9272 arg3Of ),(
R7546 T9416 T9409 arg2Of sites,to
R7547 T9416 T9410 arg1Of sites,the
R7548 T9416 T9411 arg1Of sites,Prf1
R7549 T9416 T9413 arg1Of sites,Gzmb
R7550 T9416 T9414 arg1Of sites,transcription
R7551 T9416 T9415 arg1Of sites,start
R7552 T9416 T9417 arg1Of sites,(
R7553 T9418 T9417 arg2Of TSS,(
R7554 T9275 T9272 arg1Of overexpression,(
R7555 T9419 T9417 arg3Of ),(
R7556 T9275 T9276 arg1Of overexpression,of
R7557 T9275 T9278 arg1Of overexpression,in
R7558 T9275 T9285 arg1Of overexpression,led
R7559 T9277 T9276 arg2Of Eo-VP16,of
R7560 T9280 T9281 arg1Of WT,or
R7561 T9281 T9278 arg2Of or,in
R7562 T9426 T9421 arg2Of enhancer,to
R7563 T9426 T9422 arg1Of enhancer,a
R7564 T9426 T9423 arg2Of enhancer,known
R7565 T9426 T9424 arg1Of enhancer,IL-2
R7566 T9426 T9425 arg1Of enhancer,responsive
R7567 T9426 T9427 arg1Of enhancer,located
R7568 T9427 T9428 arg1Of located,near
R7569 T9281 T9279 arg1Of or,either
R7570 T9430 T9428 arg2Of kb,near
R7571 T9430 T9429 arg1Of kb,−1
R7572 T9430 T9431 arg1Of kb,of
R7573 T9284 T9281 arg2Of cells,or
R7574 T9434 T9431 arg2Of gene,of
R7575 T9434 T9432 arg1Of gene,the
R7576 T9434 T9433 arg1Of gene,Prf1
R7577 T9434 T9435 arg1Of gene,(
R7578 T9436 T9435 arg2Of 23,(
R7579 T9437 T9435 arg3Of ),(
R7580 T9284 T9282 arg1Of cells,T-bet–deficient
R7581 T9284 T9283 arg1Of cells,T
R7582 T9285 T9271 arg2Of led,and
R7583 T9285 T9286 arg1Of led,to
R7584 T9288 T9286 arg2Of increase,to
R7585 T9446 T9439 arg2Of site,to
R7586 T9446 T9440 arg1Of site,the
R7587 T9446 T9441 arg1Of site,distal
R7588 T9446 T9442 arg1Of site,CTL-specific
R7589 T9446 T9443 arg1Of site,DNase
R7590 T9446 T9444 arg1Of site,I
R7591 T9446 T9445 arg1Of site,hypersensitive
R7592 T9446 T9447 arg1Of site,9
R7593 T9446 T9448 arg1Of site,in
R7594 T9451 T9448 arg2Of locus,in
R7595 T9451 T9449 arg1Of locus,the
R7596 T9451 T9450 arg1Of locus,Prf1
R7597 T9451 T9452 arg1Of locus,(
R7598 T9288 T9287 arg1Of increase,an
R7599 T9453 T9452 arg2Of 24,(
R7600 T9288 T9289 arg1Of increase,in
R7601 T9454 T9452 arg3Of ),(
R7602 T9291 T9292 arg1Of perforin,and
R7603 T9459 T9456 arg2Of promoter,to
R7604 T9459 T9457 arg1Of promoter,the
R7605 T9459 T9458 arg1Of promoter,Ifng
R7606 T9459 T9460 arg1Of promoter,near
R7607 T9459 T9466 arg2Of promoter,reported
R7608 T9292 T9290 arg1Of and,both
R7609 T9462 T9460 arg2Of TSS,near
R7610 T9462 T9461 arg1Of TSS,the
R7611 T9293 T9292 arg2Of IFN-γ,and
R7612 T9465 T9464 arg1Of previously,as
R7613 T9466 T9463 arg1Of reported,","
R7614 T9466 T9465 arg1Of reported,previously
R7615 T9466 T9467 arg1Of reported,for
R7616 T9466 T9475 arg1Of reported,to
R7617 T9467 T9474 arg1Of for,and
R7618 T9469 T9467 arg2Of cells,for
R7619 T9469 T9468 arg1Of cells,Th1
R7620 T9469 T9470 arg1Of cells,(
R7621 T9294 T9289 arg2Of expression,in
R7622 T9471 T9470 arg2Of 10,(
R7623 T9472 T9470 arg3Of ),(
R7624 T9294 T9291 arg1Of expression,perforin
R7625 T9474 T9473 arg1Of and,;
R7626 T9475 T9474 arg2Of to,and
R7627 T9294 T9293 arg1Of expression,IFN-γ
R7628 T9294 T9295 arg1Of expression,(
R7629 T9296 T9297 arg1Of Fig.,2
R7630 T9480 T9475 arg2Of sites,to
R7631 T9480 T9476 arg1Of sites,several
R7632 T9480 T9477 arg1Of sites,DNase
R7633 T9480 T9478 arg1Of sites,I
R7634 T9480 T9479 arg1Of sites,hypersensitive
R7635 T9480 T9481 arg1Of sites,in
R7636 T9296 T9298 arg1Of Fig.,","
R7637 T9480 T9493 arg1Of sites,(
R7638 T9298 T9300 arg1Of ",",and
R7639 T9299 T9298 arg2Of B,","
R7640 T9484 T9481 arg2Of locus,in
R7641 T9484 T9482 arg1Of locus,the
R7642 T9484 T9483 arg1Of locus,Ifng
R7643 T9484 T9485 arg1Of locus,(
R7644 T9300 T9295 arg2Of and,(
R7645 T9488 T9485 arg2Of D,(
R7646 T9488 T9486 arg1Of D,Fig.
R7647 T9488 T9487 arg1Of D,3
R7648 T9488 T9491 arg1Of D,depicted
R7649 T9491 T9489 arg1Of depicted,and
R7650 T9491 T9490 arg1Of depicted,not
R7651 T9301 T9300 arg2Of D,and
R7652 T9492 T9485 arg3Of ),(
R7653 T9494 T9493 arg2Of 25,(
R7654 T9302 T9295 arg3Of ),(
R7655 T9495 T9493 arg3Of ),(
R7656 T9496 T9497 arg1Of Eomes,bound
R7657 T9304 T9305 arg1Of we,asked
R7658 T9497 T9499 arg1Of bound,to
R7659 T9497 T9508 arg1Of bound,;
R7660 T9499 T9498 arg1Of to,primarily
R7661 T9305 T9237 arg1Of asked,Because
R7662 T9502 T9500 arg1Of TSS,the
R7663 T9502 T9501 arg1Of TSS,Prf1
R7664 T9502 T9503 arg1Of TSS,and
R7665 T9503 T9499 arg2Of and,to
R7666 T9305 T9303 arg1Of asked,","
R7667 T9308 T9307 arg1Of differentiation,CTL
R7668 T9507 T9503 arg2Of enhancer,and
R7669 T9507 T9504 arg1Of enhancer,the
R7670 T9507 T9505 arg1Of enhancer,−1
R7671 T9507 T9506 arg1Of enhancer,kb
R7672 T9510 T9509 arg1Of binding,this
R7673 T9510 T9511 arg1Of binding,was
R7674 T9510 T9513 arg1Of binding,greater
R7675 T9510 T9536 arg1Of binding,comparable
R7676 T9511 T9508 arg2Of was,;
R7677 T9513 T9512 arg1Of greater,substantially
R7678 T9513 T9514 arg1Of greater,than
R7679 T9513 T9535 arg1Of greater,and
R7680 T9515 T9514 arg2Of that,than
R7681 T9515 T9516 arg2Of that,observed
R7682 T9515 T9524 arg1Of that,","
R7683 T9308 T9309 arg1Of differentiation,was
R7684 T9516 T9517 arg1Of observed,at
R7685 T9308 T9312 arg2Of differentiation,regulated
R7686 T9312 T9305 arg2Of regulated,asked
R7687 T9519 T9517 arg2Of promoter,at
R7688 T9519 T9518 arg1Of promoter,the
R7689 T9519 T9520 arg1Of promoter,of
R7690 T9312 T9306 arg1Of regulated,whether
R7691 T9312 T9309 arg2Of regulated,was
R7692 T9312 T9310 arg1Of regulated,also
R7693 T9312 T9311 arg1Of regulated,potentially
R7694 T9316 T9312 arg1Of loop,regulated
R7695 T9523 T9520 arg2Of gene,of
R7696 T9523 T9521 arg1Of gene,the
R7697 T9523 T9522 arg1Of gene,Il2rb
R7698 T9316 T9313 arg2Of loop,by
R7699 T9316 T9314 arg1Of loop,a
R7700 T9528 T9524 arg2Of target,","
R7701 T9528 T9525 arg1Of target,a
R7702 T9528 T9526 arg1Of target,known
R7703 T9528 T9527 arg1Of target,direct
R7704 T9528 T9529 arg1Of target,of
R7705 T9316 T9315 arg1Of loop,feed-forward
R7706 T9530 T9529 arg2Of Eomes,of
R7707 T9530 T9531 arg1Of Eomes,(
R7708 T9316 T9317 arg1Of loop,involving
R7709 T9532 T9531 arg2Of 8,(
R7710 T9321 T9317 arg2Of classes,involving
R7711 T9533 T9531 arg3Of ),(
R7712 T9535 T9511 arg2Of and,was
R7713 T9535 T9534 arg1Of and,","
R7714 T9536 T9535 arg2Of comparable,and
R7715 T9536 T9537 arg1Of comparable,to
R7716 T9538 T9537 arg2Of that,to
R7717 T9538 T9539 arg2Of that,observed
R7718 T9538 T9544 arg1Of that,","
R7719 T9539 T9540 arg1Of observed,at
R7720 T9321 T9318 arg1Of classes,these
R7721 T9321 T9319 arg1Of classes,same
R7722 T9543 T9540 arg2Of TSS,at
R7723 T9543 T9541 arg1Of TSS,the
R7724 T9543 T9542 arg1Of TSS,Ifng
R7725 T9321 T9320 arg1Of classes,two
R7726 T9321 T9322 arg1Of classes,of
R7727 T9547 T9544 arg2Of target,","
R7728 T9547 T9545 arg1Of target,a
R7729 T9547 T9546 arg2Of target,known
R7730 T9547 T9548 arg1Of target,of
R7731 T9547 T9551 arg1Of target,in
R7732 T9323 T9324 arg1Of Runx,and
R7733 T9547 T9563 arg1Of target,(
R7734 T9550 T9548 arg2Of proteins,of
R7735 T9550 T9549 arg1Of proteins,T-box
R7736 T9325 T9324 arg2Of T-box,and
R7737 T9327 T9322 arg2Of factors,of
R7738 T9557 T9551 arg2Of cells,in
R7739 T9557 T9552 arg1Of cells,both
R7740 T9557 T9553 arg1Of cells,Th1
R7741 T9557 T9554 arg1Of cells,and
R7742 T9557 T9555 arg1Of cells,CD8+
R7743 T9557 T9556 arg1Of cells,T
R7744 T9557 T9558 arg1Of cells,(
R7745 T9327 T9323 arg1Of factors,Runx
R7746 T9327 T9325 arg1Of factors,T-box
R7747 T9561 T9558 arg2Of D,(
R7748 T9561 T9559 arg1Of D,Fig.
R7749 T9561 T9560 arg1Of D,3
R7750 T9562 T9558 arg3Of ),(
R7751 T9327 T9326 arg1Of factors,transcription
R7752 T9622 T9619 arg2Of cells,and
R7753 T9564 T9563 arg2Of 17,(
R7754 T9565 T9563 arg3Of ),(
R7755 T9567 T9566 arg1Of determine,To
R7756 T9569 T9570 arg1Of Runx3,controlled
R7757 T9570 T9567 arg2Of controlled,determine
R7758 T9570 T9568 arg1Of controlled,whether
R7759 T9570 T9577 arg1Of controlled,through
R7760 T9622 T9620 arg1Of cells,CD8+CD4+
R7761 T9572 T9570 arg2Of expression,controlled
R7762 T9572 T9571 arg1Of expression,the
R7763 T9572 T9573 arg1Of expression,of
R7764 T9622 T9621 arg1Of cells,DP
R7765 T9626 T9623 arg2Of experiments,in
R7766 T9576 T9573 arg2Of genes,of
R7767 T9576 T9574 arg1Of genes,CTL
R7768 T9576 T9575 arg1Of genes,effector
R7769 T9579 T9577 arg2Of induction,through
R7770 T9579 T9578 arg1Of induction,its
R7771 T9579 T9580 arg1Of induction,of
R7772 T9581 T9580 arg2Of Eomes,of
R7773 T9583 T9567 arg1Of we,determine
R7774 T9583 T9585 arg1Of we,expressed
R7775 T9585 T9566 modOf expressed,To
R7776 T9585 T9582 arg1Of expressed,","
R7777 T9585 T9584 arg1Of expressed,retrovirally
R7778 T9585 T9589 arg1Of expressed,in
R7779 T9586 T9587 arg1Of Runx3,and
R7780 T9626 T9624 arg1Of experiments,our
R7781 T9587 T9585 arg2Of and,expressed
R7782 T9626 T9625 arg1Of experiments,previous
R7783 T9588 T9587 arg2Of Eo-VP16,and
R7784 T9628 T9629 arg1Of we,used
R7785 T9592 T9589 arg2Of cells,in
R7786 T9592 T9590 arg1Of cells,CD8+
R7787 T9592 T9591 arg1Of cells,T
R7788 T9592 T9593 arg1Of cells,from
R7789 T9629 T9596 arg1Of used,Because
R7790 T9595 T9593 arg2Of mice,from
R7791 T9595 T9594 arg1Of mice,Runx3−/−
R7792 T9596 T9597 arg1Of Because,of
R7793 T9596 T9609 arg1Of Because,and
R7794 T9629 T9610 arg1Of used,because
R7795 T9629 T9627 arg1Of used,","
R7796 T9600 T9596 arg2Of number,Because
R7797 T9600 T9598 arg1Of number,the
R7798 T9600 T9599 arg1Of number,limited
R7799 T9600 T9601 arg1Of number,of
R7800 T9600 T9605 arg1Of number,in
R7801 T9629 T9635 arg1Of used,without
R7802 T9629 T9638 arg1Of used,as
R7803 T9604 T9601 arg2Of cells,of
R7804 T9604 T9602 arg1Of cells,CD8+
R7805 T9604 T9603 arg1Of cells,T
R7806 T9634 T9629 arg2Of cells,used
R7807 T9607 T9605 arg2Of mice,in
R7808 T9607 T9606 arg1Of mice,these
R7809 T9609 T9608 arg1Of and,","
R7810 T9610 T9609 arg2Of because,and
R7811 T9611 T9612 arg1Of we,saw
R7812 T9612 T9610 arg2Of saw,because
R7813 T9612 T9623 arg1Of saw,in
R7814 T9634 T9630 arg1Of cells,total
R7815 T9614 T9612 arg2Of difference,saw
R7816 T9634 T9631 arg1Of cells,Runx3−/−
R7817 T9634 T9632 arg1Of cells,CD8+
R7818 T9634 T9633 arg1Of cells,T
R7819 T9637 T9635 arg2Of fractionation,without
R7820 T9614 T9613 arg1Of difference,no
R7821 T9614 T9615 arg1Of difference,between
R7822 T9618 T9616 arg1Of SP,Runx3−/−
R7823 T9618 T9617 arg1Of SP,CD8+CD4−
R7824 T9618 T9619 arg1Of SP,and
R7825 T9619 T9615 arg2Of and,between
R7826 T9637 T9636 arg1Of fractionation,further
R7827 T9639 T9638 arg2Of recipients,as
R7828 T9639 T9640 arg1Of recipients,for
R7829 T9642 T9640 arg2Of transduction,for
R7830 T9719 T9718 arg1Of though,even
R7831 T9642 T9641 arg1Of transduction,retroviral
R7832 T9643 T9644 arg1Of Reconstitution,of
R7833 T9720 T9721 arg1Of it,restored
R7834 T9721 T9719 arg2Of restored,though
R7835 T9643 T9649 arg1Of Reconstitution,with
R7836 T9643 T9651 arg1Of Reconstitution,restored
R7837 T9723 T9721 arg2Of capacity,restored
R7838 T9723 T9722 arg1Of capacity,the
R7839 T9723 T9724 modOf capacity,to
R7840 T9648 T9644 arg2Of cells,of
R7841 T9725 T9724 arg1Of induce,to
R7842 T9725 T9728 arg1Of induce,upon
R7843 T9727 T9725 arg2Of expression,induce
R7844 T9727 T9726 arg1Of expression,IFN-γ
R7845 T9730 T9728 arg2Of restimulation,upon
R7846 T9730 T9729 arg1Of restimulation,TCR
R7847 T9730 T9731 arg1Of restimulation,(
R7848 T9732 T9731 arg2Of Fig.,(
R7849 T9732 T9733 arg1Of Fig.,4
R7850 T9732 T9734 arg1Of Fig.,","
R7851 T9648 T9645 arg1Of cells,Runx3−/−
R7852 T9648 T9646 arg1Of cells,CD8+
R7853 T9735 T9736 arg1Of A,and
R7854 T9648 T9647 arg1Of cells,T
R7855 T9736 T9734 arg2Of and,","
R7856 T9650 T9649 arg2Of Runx3,with
R7857 T9737 T9736 arg2Of B,and
R7858 T9738 T9731 arg3Of ),(
R7859 T9740 T9739 arg1Of result,This
R7860 T9740 T9741 arg1Of result,suggests
R7861 T9741 T9742 arg1Of suggests,strongly
R7862 T9652 T9651 arg2Of expression,restored
R7863 T9652 T9653 arg1Of expression,of
R7864 T9745 T9744 arg1Of expression,perforin
R7865 T9745 T9746 arg1Of expression,requires
R7866 T9746 T9741 arg2Of requires,suggests
R7867 T9746 T9743 arg1Of requires,that
R7868 T9652 T9665 arg1Of expression,(
R7869 T9747 T9748 arg1Of Runx3,and
R7870 T9748 T9746 arg2Of and,requires
R7871 T9749 T9748 arg2Of Eomes,and
R7872 T9654 T9657 arg1Of Eomes,as
R7873 T9751 T9750 arg2Of expected,As
R7874 T9751 T9752 arg1Of expected,from
R7875 T9657 T9653 arg2Of as,of
R7876 T9754 T9752 arg2Of defect,from
R7877 T9754 T9753 arg1Of defect,their
R7878 T9754 T9755 arg1Of defect,in
R7879 T9756 T9757 arg1Of perforin,and
R7880 T9758 T9757 arg2Of granzyme,and
R7881 T9760 T9755 arg2Of expression,in
R7882 T9760 T9756 arg1Of expression,perforin
R7883 T9760 T9758 arg1Of expression,granzyme
R7884 T9760 T9759 arg1Of expression,B
R7885 T9657 T9655 arg1Of as,as
R7886 T9657 T9656 arg1Of as,well
R7887 T9658 T9659 arg1Of perforin,","
R7888 T9765 T9762 arg1Of cells,Runx3−/−
R7889 T9765 T9763 arg1Of cells,CD8+
R7890 T9765 T9764 arg1Of cells,T
R7891 T9765 T9766 arg1Of cells,showed
R7892 T9766 T9750 arg1Of showed,As
R7893 T9766 T9761 arg1Of showed,","
R7894 T9766 T9770 arg1Of showed,in
R7895 T9768 T9767 arg1Of cytolytic,defective
R7896 T9659 T9663 arg1Of ",",and
R7897 T9769 T9766 arg2Of activity,showed
R7898 T9769 T9768 arg1Of activity,cytolytic
R7899 T9661 T9659 arg2Of B,","
R7900 T9774 T9770 arg2Of reaction,in
R7901 T9774 T9771 arg1Of reaction,a
R7902 T9774 T9772 arg1Of reaction,mixed
R7903 T9774 T9773 arg1Of reaction,lymphocyte
R7904 T9774 T9775 arg1Of reaction,(
R7905 T9776 T9775 arg2Of 12,(
R7906 T9661 T9660 arg1Of B,granzyme
R7907 T9777 T9775 arg3Of ),(
R7908 T9663 T9657 arg2Of and,as
R7909 T9663 T9662 arg1Of and,","
R7910 T9664 T9663 arg2Of IFN-γ,and
R7911 T9783 T9780 arg1Of cells,TCR-stimulated
R7912 T9783 T9781 arg1Of cells,Runx3−/−
R7913 T9783 T9782 arg1Of cells,CD8+
R7914 T9783 T9784 arg1Of cells,were
R7915 T9783 T9786 arg1Of cells,effective
R7916 T9784 T9778 arg1Of were,However
R7917 T9784 T9779 arg1Of were,","
R7918 T9786 T9784 arg2Of effective,were
R7919 T9786 T9785 arg1Of effective,as
R7920 T9786 T9787 arg1Of effective,as
R7921 T9789 T9787 arg2Of cells,as
R7922 T9666 T9665 arg2Of Fig.,(
R7923 T9789 T9788 arg1Of cells,WT
R7924 T9789 T9790 arg1Of cells,in
R7925 T9666 T9667 arg1Of Fig.,4
R7926 T9791 T9790 arg2Of killing,in
R7927 T9791 T9794 arg1Of killing,in
R7928 T9666 T9668 arg1Of Fig.,","
R7929 T9669 T9670 arg1Of A,and
R7930 T9793 T9791 arg2Of cells,killing
R7931 T9793 T9792 arg1Of cells,tumor
R7932 T9670 T9668 arg2Of and,","
R7933 T9798 T9794 arg2Of assay,in
R7934 T9798 T9795 arg1Of assay,a
R7935 T9798 T9796 arg1Of assay,redirected
R7936 T9798 T9797 arg1Of assay,CTL
R7937 T9798 T9799 arg1Of assay,(
R7938 T9800 T9799 arg2Of 12,(
R7939 T9671 T9670 arg2Of B,and
R7940 T9801 T9799 arg3Of ),(
R7941 T9672 T9665 arg3Of ),(
R7942 T9805 T9804 arg1Of cells,CD8+
R7943 T9805 T9806 arg1Of cells,from
R7944 T9805 T9813 arg2Of cells,immunized
R7945 T9805 T9819 arg1Of cells,killed
R7946 T9674 T9673 arg2Of addition,In
R7947 T9809 T9806 arg2Of cavity,from
R7948 T9809 T9807 arg1Of cavity,the
R7949 T9809 T9808 arg1Of cavity,peritoneal
R7950 T9809 T9810 arg1Of cavity,of
R7951 T9678 T9676 arg1Of cells,Runx3−/−
R7952 T9812 T9810 arg2Of mice,of
R7953 T9812 T9811 arg1Of mice,Runx3−/−
R7954 T9813 T9814 arg1Of immunized,with
R7955 T9678 T9677 arg1Of cells,T
R7956 T9678 T9679 arg1Of cells,showed
R7957 T9679 T9673 arg1Of showed,In
R7958 T9679 T9675 arg1Of showed,","
R7959 T9679 T9693 arg1Of showed,","
R7960 T9679 T9694 modOf showed,indicating
R7961 T9817 T9814 arg2Of cells,with
R7962 T9817 T9815 arg1Of cells,certain
R7963 T9817 T9816 arg1Of cells,tumor
R7964 T9682 T9679 arg2Of up-regulation,showed
R7965 T9819 T9802 arg1Of killed,Furthermore
R7966 T9819 T9803 arg1Of killed,","
R7967 T9819 T9818 arg1Of killed,effectively
R7968 T9821 T9819 arg2Of targets,killed
R7969 T9821 T9820 arg1Of targets,these
R7970 T9821 T9822 arg1Of targets,(
R7971 T9823 T9822 arg2Of 13,(
R7972 T9824 T9822 arg3Of ),(
R7973 T9682 T9680 arg1Of up-regulation,a
R7974 T9682 T9681 arg1Of up-regulation,compensatory
R7975 T9828 T9829 arg1Of activation,of
R7976 T9682 T9683 arg1Of up-regulation,of
R7977 T9828 T9834 arg1Of activation,is
R7978 T9828 T9835 arg1Of activation,defective
R7979 T9682 T9685 arg1Of up-regulation,","
R7980 T9682 T9686 arg1Of up-regulation,which
R7981 T9682 T9687 arg1Of up-regulation,was
R7982 T9833 T9829 arg2Of machinery,of
R7983 T9833 T9830 arg1Of machinery,the
R7984 T9833 T9831 arg1Of machinery,perforin/granzyme
R7985 T9833 T9832 arg1Of machinery,B
R7986 T9834 T9827 arg2Of is,although
R7987 T9682 T9688 arg2Of up-regulation,suppressed
R7988 T9835 T9834 arg2Of defective,is
R7989 T9835 T9836 arg1Of defective,in
R7990 T9684 T9683 arg2Of Runx1,of
R7991 T9839 T9836 arg2Of cells,in
R7992 T9839 T9837 arg1Of cells,Runx3−/−
R7993 T9839 T9838 arg1Of cells,CD8+
R7994 T9688 T9687 arg2Of suppressed,was
R7995 T9842 T9841 arg1Of cells,these
R7996 T9842 T9843 arg1Of cells,are
R7997 T9842 T9846 arg1Of cells,devoid
R7998 T9842 T9851 arg1Of cells,could
R7999 T9842 T9854 arg1Of cells,kill
R8000 T9843 T9844 arg1Of are,not
R8001 T9843 T9850 arg1Of are,and
R8002 T9846 T9843 arg2Of devoid,are
R8003 T9846 T9845 arg1Of devoid,entirely
R8004 T9846 T9847 arg1Of devoid,of
R8005 T9688 T9689 arg1Of suppressed,upon
R8006 T9849 T9847 arg2Of activity,of
R8007 T9849 T9848 arg1Of activity,cytolytic
R8008 T9850 T9825 arg1Of and,Therefore
R8009 T9850 T9826 arg1Of and,","
R8010 T9850 T9827 arg1Of and,although
R8011 T9850 T9840 arg1Of and,","
R8012 T9850 T9856 arg1Of and,","
R8013 T9850 T9858 arg1Of and,by
R8014 T9690 T9689 arg2Of reconstitution,upon
R8015 T9854 T9850 arg2Of kill,and
R8016 T9854 T9851 arg2Of kill,could
R8017 T9854 T9852 arg1Of kill,still
R8018 T9854 T9853 arg1Of kill,effectively
R8019 T9855 T9854 arg2Of targets,kill
R8020 T9690 T9691 arg1Of reconstitution,with
R8021 T9858 T9857 arg1Of by,possibly
R8022 T9860 T9858 arg2Of mechanisms,by
R8023 T9860 T9859 arg1Of mechanisms,alternative
R8024 T9692 T9691 arg2Of Runx3,with
R8025 T9860 T9862 arg1Of mechanisms,as
R8026 T9696 T9697 arg1Of Runx1,is
R8027 T9862 T9861 arg1Of as,such
R8028 T9866 T9862 arg2Of pathway,as
R8029 T9866 T9863 arg1Of pathway,the
R8030 T9866 T9864 arg1Of pathway,Fas–Fas
R8031 T9866 T9865 arg1Of pathway,ligand
R8032 T9697 T9694 arg2Of is,indicating
R8033 T9697 T9695 arg1Of is,that
R8034 T9699 T9697 arg2Of target,is
R8035 T9699 T9698 arg1Of target,a
R8036 T9699 T9700 arg1Of target,of
R8037 T9699 T9702 arg1Of target,by
R8038 T9701 T9700 arg2Of repression,of
R8039 T9703 T9702 arg2Of Runx3,by
R8040 T9706 T9707 arg1Of Eo-VP16,did
R8043 T9706 T9709 arg1Of Eo-VP16,up-regulate
R8044 T9709 T9704 arg1Of up-regulate,Notably
R8047 T9709 T9705 arg1Of up-regulate,","
R8050 T9709 T9707 arg2Of up-regulate,did
R8051 T9709 T9708 arg1Of up-regulate,not
R8052 T9709 T9712 arg1Of up-regulate,when
R8053 T9709 T9717 arg1Of up-regulate,","
R8054 T9709 T9719 arg1Of up-regulate,though
R8055 T9711 T9709 arg2Of expression,up-regulate
R8056 T9711 T9710 arg1Of expression,perforin
R8057 T9713 T9712 arg2Of expressed,when
R8058 T9713 T9714 arg1Of expressed,in
R8059 T9716 T9714 arg2Of cells,in
R8060 T9716 T9715 arg1Of cells,Runx3−/−
R10973 T14019 T14020 arg1Of Antibodies,and
R10974 T14021 T14020 arg2Of reagents,and
R10975 T14024 T14022 arg1Of antibodies,The
R10976 T14024 T14023 arg1Of antibodies,following
R10977 T14024 T14025 arg2Of antibodies,used
R10978 T14024 T14031 arg1Of antibodies,were
R10979 T14024 T14032 arg2Of antibodies,obtained
R10980 T14025 T14026 arg1Of used,for
R10981 T14030 T14026 arg2Of stains,for
R10982 T14030 T14027 arg1Of stains,intracellular
R10983 T14030 T14028 arg1Of stains,or
R10984 T14030 T14029 arg1Of stains,surface
R10985 T14032 T14031 arg2Of obtained,were
R10986 T14032 T14033 arg1Of obtained,from
R10987 T14032 T14035 arg1Of obtained,:
R10988 T14032 T14036 arg1Of obtained,anti–IL-2
R10989 T14034 T14033 arg2Of eBioscience,from
R10990 T14036 T14037 arg1Of anti–IL-2,","
R10991 T14038 T14039 arg1Of anti–IFN-γ,","
R10992 T14039 T14041 arg1Of ",",","
R10993 T14040 T14039 arg2Of anti-TNF,","
R10994 T14042 T14041 arg2Of anti–granzyme,","
R10995 T14043 T14044 arg1Of B,","
R10996 T14044 T14046 arg1Of ",",","
R10997 T14045 T14044 arg2Of anti-CD8,","
R10998 T14046 T14049 arg1Of ",",and
R10999 T14047 T14046 arg2Of anti-CD25,","
R11000 T14049 T14037 arg2Of and,","
R11001 T14049 T14038 arg1Of and,anti–IFN-γ
R11002 T14049 T14040 arg1Of and,anti-TNF
R11003 T14049 T14042 arg1Of and,anti–granzyme
R11004 T14049 T14048 arg1Of and,","
R11005 T14050 T14049 arg2Of anti-CD44,and
R11006 T14051 T14052 arg1Of Anti-CD69,was
R11007 T14051 T14053 arg2Of Anti-CD69,purchased
R11008 T14053 T14052 arg2Of purchased,was
R11009 T14053 T14054 arg1Of purchased,from
R11010 T14055 T14054 arg2Of BD,from
R11011 T14058 T14056 arg2Of experiments,For
R11012 T14058 T14057 arg1Of experiments,ChIP
R11013 T14062 T14060 arg1Of antibody,the
R11014 T14062 T14061 arg1Of antibody,anti-Eomes
R11015 T14062 T14063 arg1Of antibody,was
R11016 T14062 T14064 arg2Of antibody,obtained
R11017 T14064 T14063 arg2Of obtained,was
R11018 T14064 T14065 arg1Of obtained,from
R11019 T14064 T14067 arg1Of obtained,and
R11020 T14066 T14065 arg2Of Abcam,from
R11021 T14067 T14056 arg1Of and,For
R11022 T14067 T14059 arg1Of and,","
R11023 T14070 T14068 arg1Of antibody,the
R11024 T14070 T14069 arg1Of antibody,anti-Runx3
R11025 T14070 T14071 arg1Of antibody,was
R11026 T14070 T14072 arg2Of antibody,produced
R11027 T14072 T14067 arg2Of produced,and
R11028 T14072 T14071 arg2Of produced,was
R11029 T14076 T14072 arg1Of laboratory,produced
R11030 T14076 T14073 arg2Of laboratory,by
R11031 T14076 T14074 arg1Of laboratory,the
R11032 T14076 T14075 arg1Of laboratory,Groner
R11033 T14079 T14077 arg1Of antibodies,The
R11034 T14079 T14078 arg1Of antibodies,following
R11035 T14079 T14080 arg1Of antibodies,were
R11036 T14079 T14081 arg2Of antibodies,used
R11037 T14081 T14080 arg2Of used,were
R11038 T14081 T14082 arg1Of used,for
R11039 T14083 T14082 arg2Of immunoblotting,for
R11040 T14083 T14084 arg1Of immunoblotting,:
R11041 T14085 T14086 arg1Of antiperforin,(
R11042 T14085 T14089 arg1Of antiperforin,","
R11043 T14087 T14086 arg2Of Abcam,(
R11044 T14088 T14086 arg3Of ),(
R11045 T14089 T14095 arg1Of ",",and
R11046 T14090 T14089 arg2Of anti-Eomes,","
R11047 T14090 T14091 arg1Of anti-Eomes,(
R11048 T14092 T14091 arg2Of Abcam,(
R11049 T14093 T14091 arg3Of ),(
R11050 T14095 T14084 arg2Of and,:
R11051 T14095 T14094 arg1Of and,","
R11052 T14096 T14095 arg2Of anti–Pol-II,and
R11053 T14096 T14097 arg1Of anti–Pol-II,(
R11054 T14100 T14097 arg2Of Biotechnology,(
R11055 T14100 T14098 arg1Of Biotechnology,Santa
R11056 T14100 T14099 arg1Of Biotechnology,Cruz
R11057 T14100 T14101 arg1Of Biotechnology,","
R11058 T14102 T14101 arg2Of Inc.,","
R11059 T14103 T14097 arg3Of ),(
R11060 T14106 T14104 arg1Of antibody,The
R11061 T14106 T14105 arg1Of antibody,T-bet
R11062 T14106 T14107 arg1Of antibody,was
R11063 T14106 T14108 arg2Of antibody,provided
R11064 T14108 T14107 arg2Of provided,was
R11065 T14111 T14108 arg1Of Glimcher,provided
R11066 T14111 T14109 arg2Of Glimcher,by
R11067 T14111 T14110 arg1Of Glimcher,L.
R11068 T14111 T14112 arg1Of Glimcher,(
R11069 T14114 T14113 arg1Of School,Harvard
R11070 T14114 T14115 arg1Of School,of
R11071 T14114 T14118 arg1Of School,","
R11072 T14117 T14115 arg2Of Health,of
R11073 T14117 T14116 arg1Of Health,Public
R11074 T14118 T14112 arg2Of ",",(
R11075 T14118 T14120 arg1Of ",",","
R11076 T14119 T14118 arg2Of Boston,","
R11077 T14121 T14120 arg2Of MA,","
R11078 T14122 T14112 arg3Of ),(
R11079 T14125 T14123 arg1Of reagents,The
R11080 T14125 T14124 arg1Of reagents,following
R11081 T14125 T14126 arg1Of reagents,were
R11082 T14125 T14127 arg2Of reagents,used
R11083 T14127 T14126 arg2Of used,were
R11084 T14127 T14128 arg1Of used,for
R11085 T14127 T14135 arg1Of used,:
R11086 T14130 T14128 arg2Of experiments,for
R11087 T14130 T14129 arg1Of experiments,the
R11088 T14130 T14131 arg2Of experiments,presented
R11089 T14131 T14132 arg1Of presented,in
R11090 T14134 T14132 arg2Of report,in
R11091 T14134 T14133 arg1Of report,this
R11092 T14138 T14137 arg1Of Apoptosis,V–FITC
R11093 T14140 T14136 arg1Of Kit,Annexin
R11094 T14140 T14138 arg1Of Kit,Apoptosis
R11095 T14140 T14139 arg1Of Kit,Detection
R11096 T14140 T14141 arg1Of Kit,(
R11097 T14140 T14144 arg1Of Kit,","
R11098 T14142 T14141 arg2Of BD,(
R11099 T14143 T14141 arg3Of ),(
R11100 T14145 T14144 arg2Of CD8,","
R11101 T14148 T14140 arg1Of Kit,Kit
R11102 T14148 T14145 arg1Of Kit,CD8
R11103 T14148 T14146 arg1Of Kit,Negative
R11104 T14148 T14147 arg1Of Kit,Isolation
R11105 T14148 T14149 arg1Of Kit,(
R11106 T14148 T14152 arg1Of Kit,","
R11107 T14150 T14149 arg2Of Invitrogen,(
R11108 T14151 T14149 arg3Of ),(
R11109 T14152 T14160 arg1Of ",",and
R11110 T14154 T14152 arg2Of MicroBeads,","
R11111 T14154 T14153 arg1Of MicroBeads,CD8
R11112 T14154 T14155 arg1Of MicroBeads,(
R11113 T14157 T14155 arg2Of Biotec,(
R11114 T14157 T14156 arg1Of Biotec,Miltenyi
R11115 T14158 T14155 arg3Of ),(
R11116 T14160 T14159 arg1Of and,","
R11117 T14165 T14160 arg2Of Reagents,and
R11118 T14165 T14161 arg1Of Reagents,SYBR
R11119 T14165 T14162 arg1Of Reagents,Green
R11120 T14165 T14163 arg1Of Reagents,PCR
R11121 T14165 T14164 arg1Of Reagents,Core
R11122 T14165 T14166 arg1Of Reagents,(
R11123 T14168 T14166 arg2Of Biosystems,(
R11124 T14168 T14167 arg1Of Biosystems,Applied
R11125 T14169 T14166 arg3Of ),(
R11126 T14172 T14170 arg1Of peptide,The
R11127 T14172 T14171 arg1Of peptide,Gp33
R11128 T14172 T14173 arg1Of peptide,(
R11129 T14172 T14176 arg1Of peptide,was
R11130 T14172 T14177 arg2Of peptide,synthesized
R11131 T14174 T14173 arg2Of KAVYNFATC,(
R11132 T14175 T14173 arg3Of ),(
R11133 T14177 T14176 arg2Of synthesized,was
R11134 T14177 T14185 arg1Of synthesized,and
R11135 T14183 T14177 arg1Of Facility,synthesized
R11136 T14183 T14178 arg2Of Facility,by
R11137 T14183 T14179 arg1Of Facility,the
R11138 T14183 T14180 arg1Of Facility,Tufts
R11139 T14183 T14181 arg1Of Facility,University
R11140 T14183 T14182 arg1Of Facility,Core
R11141 T14185 T14184 arg1Of and,","
R11142 T14187 T14186 arg1Of mM,10
R11143 T14187 T14188 arg1Of mM,of
R11144 T14187 T14191 arg1Of mM,was
R11145 T14187 T14192 arg2Of mM,prepared
R11146 T14190 T14188 arg2Of solutions,of
R11147 T14190 T14189 arg1Of solutions,stock
R11148 T14192 T14185 arg2Of prepared,and
R11149 T14192 T14191 arg2Of prepared,was
R11150 T14192 T14193 arg1Of prepared,in
R11151 T14194 T14193 arg2Of DMSO,in
R11380 T14607 T14608 arg1Of Isolation,and
R11381 T14608 T14610 arg1Of and,of
R11382 T14609 T14608 arg2Of culture,and
R11383 T14614 T14610 arg2Of cells,of
R11384 T14614 T14611 arg1Of cells,primary
R11385 T14614 T14612 arg1Of cells,CD8+
R11386 T14614 T14613 arg1Of cells,T
R11387 T14617 T14615 arg1Of cells,CD8+
R11388 T14617 T14616 arg1Of cells,T
R11389 T14617 T14618 arg1Of cells,from
R11390 T14617 T14624 arg1Of cells,transgenic
R11391 T14617 T14628 arg1Of cells,","
R11392 T14623 T14618 arg2Of TCR,from
R11393 T14623 T14619 arg1Of TCR,4–8-wk-old
R11394 T14623 T14620 arg1Of TCR,Tcra−/−
R11395 T14623 T14621 arg1Of TCR,×
R11396 T14623 T14622 arg1Of TCR,P14
R11397 T14624 T14625 arg1Of transgenic,(
R11398 T14626 T14625 arg2Of Taconic,(
R11399 T14627 T14625 arg3Of ),(
R11400 T14628 T14632 arg1Of ",",or
R11401 T14630 T14628 arg2Of WT,","
R11402 T14630 T14629 arg1Of WT,C57BL/6J
R11403 T14632 T14631 arg1Of or,","
R11404 T14632 T14640 arg1Of or,were
R11405 T14632 T14641 arg2Of or,purified
R11406 T14633 T14634 arg1Of Tbx21−/−,(
R11407 T14637 T14634 arg2Of Laboratory,(
R11408 T14637 T14635 arg1Of Laboratory,The
R11409 T14637 T14636 arg1Of Laboratory,Jackson
R11410 T14638 T14634 arg3Of ),(
R11411 T14639 T14632 arg2Of mice,or
R11412 T14639 T14633 arg1Of mice,Tbx21−/−
R11413 T14641 T14640 arg2Of purified,were
R11414 T14641 T14642 arg1Of purified,(
R11415 T14641 T14648 arg1Of purified,by
R11416 T14641 T14654 arg1Of purified,from
R11417 T14644 T14643 arg1Of 95,>
R11418 T14644 T14645 arg1Of 95,%
R11419 T14646 T14642 arg2Of purity,(
R11420 T14646 T14644 arg1Of purity,95
R11421 T14647 T14642 arg3Of ),(
R11422 T14650 T14648 arg2Of selection,by
R11423 T14650 T14649 arg1Of selection,negative
R11424 T14650 T14651 arg1Of selection,(
R11425 T14652 T14651 arg2Of Invitrogen,(
R11426 T14653 T14651 arg3Of ),(
R11427 T14656 T14657 arg1Of spleen,and
R11428 T14658 T14657 arg2Of lymph,and
R11429 T14660 T14654 arg2Of cells,from
R11430 T14660 T14655 arg2Of cells,pooled
R11431 T14660 T14656 arg1Of cells,spleen
R11432 T14660 T14658 arg1Of cells,lymph
R11433 T14660 T14659 arg1Of cells,node
R11434 T14663 T14661 arg1Of cells,CD8+
R11435 T14663 T14662 arg1Of cells,T
R11436 T14663 T14664 arg1Of cells,from
R11437 T14663 T14671 arg1Of cells,were
R11438 T14663 T14672 arg2Of cells,purified
R11439 T14666 T14664 arg2Of mice,from
R11440 T14666 T14665 arg1Of mice,Runx3−/−
R11441 T14666 T14667 arg1Of mice,on
R11442 T14670 T14667 arg2Of background,on
R11443 T14670 T14668 arg1Of background,the
R11444 T14670 T14669 arg1Of background,ICR
R11445 T14672 T14671 arg2Of purified,were
R11446 T14675 T14672 arg1Of selection,purified
R11447 T14675 T14673 arg2Of selection,by
R11448 T14675 T14674 arg1Of selection,positive
R11449 T14675 T14676 arg1Of selection,(
R11450 T14678 T14676 arg2Of Biotec,(
R11451 T14678 T14677 arg1Of Biotec,Miltenyi
R11452 T14679 T14676 arg3Of ),(
R11453 T14681 T14680 arg1Of mice,All
R11454 T14681 T14682 arg1Of mice,were
R11455 T14681 T14683 arg2Of mice,maintained
R11456 T14681 T14690 arg2Of mice,used
R11457 T14683 T14684 arg1Of maintained,in
R11458 T14683 T14689 arg1Of maintained,and
R11459 T14688 T14684 arg2Of facilities,in
R11460 T14688 T14685 arg1Of facilities,specific
R11461 T14688 T14686 arg1Of facilities,pathogen-free
R11462 T14688 T14687 arg1Of facilities,barrier
R11463 T14689 T14682 arg2Of and,were
R11464 T14689 T14712 arg1Of and,","
R11465 T14690 T14689 arg2Of used,and
R11466 T14690 T14691 arg1Of used,according
R11467 T14690 T14710 arg1Of used,For
R11468 T14692 T14691 arg2Of to,according
R11469 T14693 T14694 arg2Of protocols,approved
R11470 T14693 T14700 arg1Of protocols,and
R11471 T14699 T14694 arg1Of Institute,approved
R11472 T14699 T14695 arg2Of Institute,by
R11473 T14699 T14696 arg1Of Institute,the
R11474 T14699 T14697 arg1Of Institute,Immune
R11475 T14699 T14698 arg1Of Institute,Disease
R11476 T14700 T14692 arg2Of and,to
R11477 T14706 T14702 arg1Of Care,Harvard
R11478 T14706 T14703 arg1Of Care,Medical
R11479 T14706 T14704 arg1Of Care,School
R11480 T14706 T14705 arg1Of Care,Animal
R11481 T14706 T14707 arg1Of Care,and
R11482 T14708 T14707 arg2Of Use,and
R11483 T14709 T14700 arg2Of Committees.,and
R11484 T14709 T14701 arg1Of Committees.,the
R11485 T14709 T14706 arg1Of Committees.,Care
R11486 T14709 T14708 arg1Of Committees.,Use
R11487 T14711 T14710 arg2Of stimulation,For
R11488 T14716 T14713 arg2Of cells,purified
R11489 T14716 T14714 arg1Of cells,CD8+
R11490 T14716 T14715 arg1Of cells,T
R11491 T14716 T14717 arg1Of cells,were
R11492 T14716 T14718 arg2Of cells,cultured
R11493 T14718 T14712 arg2Of cultured,","
R11494 T14718 T14717 arg2Of cultured,were
R11495 T14718 T14719 arg1Of cultured,at
R11496 T14721 T14719 arg2Of cells/ml,at
R11497 T14721 T14720 arg1Of cells/ml,106
R11498 T14721 T14722 arg1Of cells/ml,(
R11499 T14721 T14726 arg1Of cells/ml,in
R11500 T14724 T14722 arg2Of ml,(
R11501 T14724 T14723 arg1Of ml,10
R11502 T14725 T14722 arg3Of ),(
R11503 T14728 T14726 arg2Of flasks,in
R11504 T14728 T14727 arg1Of flasks,T25
R11505 T14728 T14729 arg2Of flasks,coated
R11506 T14729 T14730 arg1Of coated,with
R11507 T14732 T14730 arg2Of μg/ml,with
R11508 T14732 T14731 arg1Of μg/ml,1
R11509 T14732 T14733 arg1Of μg/ml,each
R11510 T14732 T14734 arg1Of μg/ml,of
R11511 T14735 T14736 arg1Of anti-CD3,(
R11512 T14735 T14740 arg1Of anti-CD3,and
R11513 T14738 T14736 arg2Of 2C11,(
R11514 T14738 T14737 arg1Of 2C11,clone
R11515 T14739 T14736 arg3Of ),(
R11516 T14740 T14734 arg2Of and,of
R11517 T14740 T14746 arg1Of and,by
R11518 T14743 T14740 arg2Of clone,and
R11519 T14743 T14741 arg1Of clone,anti-CD28
R11520 T14743 T14742 arg2Of clone,(
R11521 T14743 T14744 arg1Of clone,37.51
R11522 T14745 T14742 arg3Of ),(
R11523 T14747 T14746 arg2Of pretreatment,by
R11524 T14747 T14748 arg1Of pretreatment,with
R11525 T14753 T14748 arg2Of IgG,with
R11526 T14753 T14749 arg1Of IgG,300
R11527 T14753 T14750 arg1Of IgG,μg/ml
R11528 T14753 T14751 arg1Of IgG,goat
R11529 T14753 T14752 arg1Of IgG,anti–hamster
R11530 T14756 T14754 arg2Of h,After
R11531 T14756 T14755 arg1Of h,48
R11532 T14758 T14759 arg1Of cells,were
R11533 T14758 T14760 arg2Of cells,removed
R11534 T14758 T14766 arg2Of cells,recultured
R11535 T14760 T14761 arg1Of removed,from
R11536 T14760 T14765 arg1Of removed,and
R11537 T14764 T14761 arg2Of stimulation,from
R11538 T14764 T14762 arg1Of stimulation,the
R11539 T14764 T14763 arg1Of stimulation,TCR
R11540 T14765 T14754 arg1Of and,After
R11541 T14765 T14757 arg1Of and,","
R11542 T14765 T14759 arg2Of and,were
R11543 T14766 T14765 arg2Of recultured,and
R11544 T14766 T14767 arg1Of recultured,at
R11545 T14769 T14767 arg2Of concentration,at
R11546 T14769 T14768 arg1Of concentration,a
R11547 T14769 T14770 arg1Of concentration,of
R11548 T14769 T14775 arg1Of concentration,in
R11549 T14773 T14772 arg1Of 105,×
R11550 T14774 T14770 arg2Of cells/ml,of
R11551 T14774 T14771 arg1Of cells/ml,5
R11552 T14774 T14773 arg1Of cells/ml,105
R11553 T14776 T14775 arg2Of media,in
R11554 T14776 T14777 arg2Of media,supplemented
R11555 T14777 T14778 arg1Of supplemented,with
R11556 T14781 T14778 arg2Of rhIL-2,with
R11557 T14781 T14779 arg1Of rhIL-2,100
R11558 T14781 T14780 arg1Of rhIL-2,U/ml
R11559 T14784 T14782 arg1Of h,Every
R11560 T14784 T14783 arg1Of h,24
R11561 T14787 T14786 arg1Of cells,viable
R11562 T14787 T14788 arg1Of cells,were
R11563 T14787 T14789 arg2Of cells,counted
R11564 T14787 T14791 arg2Of cells,readjusted
R11565 T14789 T14790 arg1Of counted,and
R11566 T14790 T14784 arg1Of and,h
R11567 T14790 T14785 arg1Of and,","
R11568 T14790 T14788 arg2Of and,were
R11569 T14791 T14790 arg2Of readjusted,and
R11570 T14791 T14792 arg1Of readjusted,to
R11571 T14795 T14794 arg1Of 105,×
R11572 T14796 T14792 arg2Of cells/ml,to
R11573 T14796 T14793 arg1Of cells/ml,5
R11574 T14796 T14795 arg1Of cells/ml,105
R11575 T14796 T14797 arg1Of cells/ml,with
R11576 T14799 T14797 arg2Of media,with
R11577 T14799 T14798 arg1Of media,fresh
R11578 T14799 T14800 arg1Of media,containing
R11579 T14803 T14800 arg2Of amount,containing
R11580 T14803 T14801 arg1Of amount,the
R11581 T14803 T14802 arg1Of amount,corresponding
R11582 T14803 T14804 arg1Of amount,of
R11583 T14805 T14804 arg2Of rhIL-2,of
R11973 T15411 T15412 arg1Of Isolation,of
R11974 T15415 T15412 arg2Of cells,of
R11975 T15415 T15413 arg1Of cells,CD8+
R11976 T15415 T15414 arg1Of cells,T
R11977 T15415 T15416 arg1Of cells,from
R11978 T15418 T15416 arg2Of mice,from
R11979 T15418 T15417 arg1Of mice,Runx3−/−
R11980 T15421 T15419 arg1Of cells,Runx3-deficient
R11981 T15421 T15420 arg1Of cells,T
R11982 T15421 T15422 arg1Of cells,fail
R11983 T15422 T15423 arg1Of fail,to
R11984 T15422 T15432 arg1Of fail,(
R11985 T15426 T15423 arg2Of expression,to
R11986 T15426 T15424 arg1Of expression,silence
R11987 T15426 T15425 arg1Of expression,CD4
R11988 T15426 T15427 arg1Of expression,normally
R11989 T15426 T15428 arg1Of expression,(
R11990 T15430 T15428 arg2Of S1,(
R11991 T15430 T15429 arg1Of S1,Fig.
R11992 T15431 T15428 arg3Of ),(
R11993 T15433 T15432 arg2Of 12,(
R11994 T15433 T15434 arg1Of 12,","
R11995 T15435 T15434 arg2Of 13,","
R11996 T15436 T15432 arg3Of ),(
R11997 T15437 T15440 arg1Of We,fractionated
R11998 T15440 T15438 arg1Of fractionated,therefore
R11999 T15440 T15439 arg1Of fractionated,further
R12000 T15440 T15451 arg1Of fractionated,into
R12001 T15440 T15460 modOf fractionated,using
R12002 T15443 T15442 arg1Of selected,positively
R12003 T15446 T15440 arg2Of cells,fractionated
R12004 T15446 T15441 arg1Of cells,the
R12005 T15446 T15443 arg1Of cells,selected
R12006 T15446 T15444 arg1Of cells,CD8+
R12007 T15446 T15445 arg1Of cells,T
R12008 T15446 T15447 arg1Of cells,from
R12009 T15450 T15447 arg2Of mice,from
R12010 T15450 T15448 arg1Of mice,Runx3
R12011 T15450 T15449 arg1Of mice,KO
R12012 T15453 T15452 arg1Of SP,CD8+CD4−
R12013 T15453 T15454 arg1Of SP,or
R12014 T15454 T15451 arg2Of or,into
R12015 T15454 T15458 arg1Of or,by
R12016 T15457 T15454 arg2Of cells,or
R12017 T15457 T15455 arg1Of cells,CD8+CD4+
R12018 T15457 T15456 arg1Of cells,DP
R12019 T15459 T15458 arg2Of separation,by
R12020 T15462 T15461 arg1Of magnetic,anti-CD4
R12021 T15463 T15460 arg2Of beads,using
R12022 T15463 T15462 arg1Of beads,magnetic
R12023 T15464 T15465 arg1Of This,yielded
R12024 T15465 T15478 arg1Of yielded,and
R12025 T15469 T15466 arg1Of SP,a
R12026 T15469 T15467 arg1Of SP,Runx3
R12027 T15469 T15468 arg1Of SP,KO
R12028 T15469 T15470 arg1Of SP,“enriched”
R12029 T15471 T15470 arg2Of population,“enriched”
R12030 T15472 T15465 arg2Of that,yielded
R12031 T15472 T15473 arg1Of that,contained
R12032 T15473 T15470 arg3Of contained,“enriched”
R12033 T15474 T15475 arg1Of 75,%
R12034 T15477 T15473 arg2Of cells,contained
R12035 T15477 T15474 arg1Of cells,75
R12036 T15477 T15476 arg1Of cells,CD8+CD4−
R12037 T15481 T15479 arg1Of DP,a
R12038 T15481 T15480 arg1Of DP,KO
R12039 T15481 T15482 arg1Of DP,enriched
R12040 T15482 T15478 arg2Of enriched,and
R12041 T15483 T15482 arg2Of population,enriched
R12042 T15483 T15484 arg1Of population,that
R12043 T15483 T15485 arg1Of population,contained
R12044 T15486 T15487 arg1Of 85,%
R12045 T15489 T15485 arg2Of cells,contained
R12046 T15489 T15486 arg1Of cells,85
R12047 T15489 T15488 arg1Of cells,CD8+CD4+
R12048 T15489 T15490 arg1Of cells,(
R12049 T15492 T15490 arg2Of S1,(
R12050 T15492 T15491 arg1Of S1,Fig.
R12051 T15493 T15490 arg3Of ),(
R12052 T15495 T15494 arg1Of cells,The
R12053 T15495 T15496 arg1Of cells,were
R12054 T15495 T15497 arg2Of cells,stimulated
R12055 T15495 T15505 arg1Of cells,removing
R12056 T15495 T15512 arg1Of cells,culturing
R12057 T15497 T15496 arg2Of stimulated,were
R12058 T15497 T15498 arg1Of stimulated,with
R12059 T15497 T15501 arg1Of stimulated,for
R12060 T15497 T15504 arg1Of stimulated,before
R12061 T15500 T15498 arg2Of anti-CD28,with
R12062 T15500 T15499 arg1Of anti-CD28,anti-CD3+
R12063 T15503 T15501 arg2Of d,for
R12064 T15503 T15502 arg1Of d,2
R12065 T15505 T15507 arg1Of removing,from
R12066 T15505 T15511 arg1Of removing,and
R12067 T15506 T15505 arg2Of them,removing
R12068 T15510 T15507 arg2Of stimulus,from
R12069 T15510 T15508 arg1Of stimulus,the
R12070 T15510 T15509 arg1Of stimulus,TCR
R12071 T15511 T15504 arg2Of and,before
R12072 T15512 T15511 arg2Of culturing,and
R12073 T15512 T15514 arg1Of culturing,in
R12074 T15513 T15512 arg2Of them,culturing
R12075 T15515 T15514 arg2Of media,in
R12076 T15515 T15516 arg1Of media,containing
R12077 T15519 T15516 arg2Of IL-2,containing
R12078 T15519 T15517 arg1Of IL-2,100
R12079 T15519 T15518 arg1Of IL-2,U/ml
R12080 T15522 T15520 arg2Of reported,As
R12081 T15522 T15521 arg1Of reported,previously
R12082 T15525 T15524 arg1Of proliferation,TCR-induced
R12083 T15525 T15526 arg1Of proliferation,of
R12084 T15525 T15531 arg1Of proliferation,was
R12085 T15525 T15533 arg1Of proliferation,impaired
R12086 T15525 T15535 arg1Of proliferation,irrespective
R12087 T15530 T15526 arg2Of cells,of
R12088 T15530 T15527 arg1Of cells,Runx3−/−
R12089 T15530 T15528 arg1Of cells,CD8+
R12090 T15530 T15529 arg1Of cells,T
R12091 T15531 T15520 arg1Of was,As
R12092 T15531 T15523 arg1Of was,","
R12093 T15531 T15543 arg1Of was,(
R12094 T15533 T15534 arg1Of impaired,","
R12095 T15534 T15531 arg2Of ",",was
R12096 T15534 T15532 arg1Of ",",severely
R12097 T15535 T15534 arg2Of irrespective,","
R12098 T15535 T15536 arg1Of irrespective,of
R12099 T15538 T15536 arg2Of expression,of
R12100 T15538 T15537 arg1Of expression,CD4
R12101 T15538 T15539 arg1Of expression,(
R12102 T15541 T15539 arg2Of S1,(
R12103 T15541 T15540 arg1Of S1,Fig.
R12104 T15542 T15539 arg3Of ),(
R12105 T15544 T15543 arg2Of 12,(
R12106 T15544 T15545 arg1Of 12,","
R12107 T15546 T15545 arg2Of 13,","
R12108 T15547 T15543 arg3Of ),(
R12109 T15552 T15550 arg1Of cells,the
R12110 T15552 T15551 arg1Of cells,Runx3−/−
R12111 T15552 T15553 arg1Of cells,showed
R12112 T15553 T15548 arg1Of showed,However
R12113 T15553 T15549 arg1Of showed,","
R12114 T15556 T15553 arg2Of patterns,showed
R12115 T15556 T15554 arg1Of patterns,cell-surface
R12116 T15556 T15555 arg1Of patterns,expression
R12117 T15556 T15557 arg1Of patterns,indicative
R12118 T15556 T15561 arg1Of patterns,","
R12119 T15556 T15562 arg1Of patterns,including
R12120 T15557 T15558 arg1Of indicative,of
R12121 T15560 T15558 arg2Of cells,of
R12122 T15560 T15559 arg2Of cells,activated
R12123 T15563 T15564 arg1Of up-regulation,of
R12124 T15563 T15566 arg1Of up-regulation,and
R12125 T15565 T15564 arg2Of CD25,of
R12126 T15566 T15562 arg2Of and,including
R12127 T15567 T15566 arg2Of CD69,and
R12128 T15567 T15568 arg1Of CD69,(
R12129 T15570 T15568 arg2Of S1,(
R12130 T15570 T15569 arg1Of S1,Fig.
R12131 T15571 T15568 arg3Of ),(
R12132 T15573 T15572 arg2Of expected,As
R12133 T15573 T15574 arg1Of expected,from
R12134 T15576 T15574 arg2Of ability,from
R12135 T15576 T15575 arg1Of ability,their
R12136 T15576 T15577 modOf ability,to
R12137 T15578 T15577 arg1Of up-regulate,to
R12138 T15579 T15578 arg2Of CD25,up-regulate
R12139 T15584 T15581 arg1Of cells,Runx3−/−
R12140 T15584 T15582 arg1Of cells,CD8+
R12141 T15584 T15583 arg1Of cells,T
R12142 T15584 T15585 arg1Of cells,responded
R12143 T15584 T15594 arg2Of cells,expanded
R12144 T15585 T15586 arg1Of responded,to
R12145 T15585 T15589 arg1Of responded,after
R12146 T15585 T15592 arg1Of responded,and
R12147 T15588 T15586 arg2Of supplementation,to
R12148 T15588 T15587 arg1Of supplementation,IL-2
R12149 T15590 T15589 arg2Of day,after
R12150 T15590 T15591 arg1Of day,2
R12151 T15592 T15572 arg1Of and,As
R12152 T15592 T15580 arg1Of and,","
R12153 T15594 T15592 arg2Of expanded,and
R12154 T15594 T15593 arg1Of expanded,efficiently
R12155 T15594 T15595 arg1Of expanded,until
R12156 T15594 T15602 arg1Of expanded,","
R12157 T15594 T15604 arg1Of expanded,at
R12158 T15596 T15595 arg2Of day,until
R12159 T15596 T15597 arg1Of day,6
R12160 T15596 T15598 arg1Of day,of
R12161 T15601 T15598 arg2Of period,of
R12162 T15601 T15599 arg1Of period,the
R12163 T15601 T15600 arg1Of period,culture
R12164 T15604 T15603 arg1Of at,albeit
R12165 T15606 T15604 arg2Of rates,at
R12166 T15606 T15605 arg1Of rates,slower
R12167 T15606 T15607 arg1Of rates,compared
R12168 T15608 T15607 arg2Of with,compared
R12169 T15610 T15608 arg2Of cells,with
R12170 T15610 T15609 arg1Of cells,WT
R12171 T15610 T15611 arg1Of cells,(
R12172 T15613 T15611 arg2Of S1,(
R12173 T15613 T15612 arg1Of S1,Fig.
R12174 T15614 T15611 arg3Of ),(
R12175 T15617 T15616 arg1Of fraction,a
R12176 T15617 T15618 arg1Of fraction,of
R12177 T15617 T15623 arg1Of fraction,silenced
R12178 T15622 T15618 arg2Of cells,of
R12179 T15622 T15619 arg1Of cells,the
R12180 T15622 T15620 arg1Of cells,KO
R12181 T15622 T15621 arg1Of cells,DP
R12182 T15623 T15615 arg2Of silenced,Although
R12183 T15623 T15626 arg1Of silenced,after
R12184 T15625 T15623 arg2Of expression,silenced
R12185 T15625 T15624 arg1Of expression,CD4
R12186 T15627 T15626 arg2Of activation,after
R12187 T15630 T15629 arg1Of ratio,the
R12188 T15630 T15631 arg1Of ratio,of
R12189 T15630 T15634 arg1Of ratio,in
R12190 T15630 T15638 arg1Of ratio,remained
R12191 T15630 T15639 arg1Of ratio,constant
R12192 T15633 T15631 arg2Of cells,of
R12193 T15633 T15632 arg1Of cells,SP/DP
R12194 T15637 T15634 arg2Of population,in
R12195 T15637 T15635 arg1Of population,each
R12196 T15637 T15636 arg2Of population,enriched
R12197 T15638 T15615 arg1Of remained,Although
R12198 T15638 T15628 arg1Of remained,","
R12199 T15638 T15640 arg1Of remained,thereafter
R12200 T15638 T15642 arg1Of remained,and
R12201 T15639 T15638 arg2Of constant,remained
R12202 T15642 T15641 arg1Of and,","
R12203 T15643 T15644 arg1Of we,did
R12204 T15643 T15646 arg1Of we,observe
R12205 T15646 T15642 arg2Of observe,and
R12206 T15646 T15644 arg2Of observe,did
R12207 T15646 T15645 arg1Of observe,not
R12208 T15646 T15658 arg1Of observe,","
R12209 T15646 T15659 modOf observe,indicating
R12210 T15649 T15646 arg2Of differences,observe
R12211 T15649 T15647 arg1Of differences,any
R12212 T15649 T15648 arg1Of differences,major
R12213 T15649 T15650 arg1Of differences,between
R12214 T15653 T15650 arg2Of populations,between
R12215 T15653 T15651 arg1Of populations,these
R12216 T15653 T15652 arg1Of populations,two
R12217 T15653 T15654 arg1Of populations,throughout
R12218 T15657 T15654 arg2Of period,throughout
R12219 T15657 T15655 arg1Of period,the
R12220 T15657 T15656 arg1Of period,culture
R12221 T15661 T15667 arg1Of in,and
R12222 T15662 T15661 arg2Of terms,in
R12223 T15662 T15663 arg1Of terms,of
R12224 T15666 T15663 arg2Of differentiation,of
R12225 T15666 T15664 arg1Of differentiation,effector
R12226 T15666 T15665 arg1Of differentiation,CTL
R12227 T15668 T15667 arg2Of under,and
R12228 T15671 T15668 arg2Of conditions,under
R12229 T15671 T15669 arg1Of conditions,our
R12230 T15671 T15670 arg1Of conditions,culture
R12231 T15676 T15673 arg1Of cells,Runx3−/−
R12232 T15676 T15674 arg1Of cells,CD8+
R12233 T15676 T15675 arg1Of cells,T
R12234 T15676 T15677 arg1Of cells,that
R12235 T15676 T15679 arg1Of cells,coexpress
R12236 T15676 T15681 arg1Of cells,are
R12237 T15676 T15682 arg1Of cells,indistinguishable
R12238 T15679 T15678 arg1Of coexpress,also
R12239 T15680 T15679 arg2Of CD4,coexpress
R12240 T15681 T15659 arg2Of are,indicating
R12241 T15681 T15660 arg1Of are,that
R12242 T15681 T15661 arg1Of are,in
R12243 T15681 T15668 arg1Of are,under
R12244 T15681 T15672 arg1Of are,","
R12245 T15682 T15681 arg2Of indistinguishable,are
R12246 T15682 T15683 arg1Of indistinguishable,from
R12247 T15684 T15683 arg2Of those,from
R12248 T15684 T15685 arg1Of those,that
R12249 T15684 T15686 arg1Of those,do
R12250 T15686 T15687 arg1Of do,not
R12251 T15689 T15688 arg1Of data,The
R12252 T15689 T15690 arg2Of data,presented
R12253 T15689 T15694 arg1Of data,are
R12254 T15689 T15709 arg1Of data,are
R12255 T15689 T15710 arg1Of data,from
R12256 T15690 T15691 arg1Of presented,in
R12257 T15693 T15691 arg2Of S2,in
R12258 T15693 T15692 arg1Of S2,Fig.
R12259 T15694 T15695 arg1Of are,from
R12260 T15694 T15700 arg1Of are,","
R12261 T15694 T15701 arg1Of are,whereas
R12262 T15699 T15695 arg2Of cells,from
R12263 T15699 T15696 arg1Of cells,Runx3
R12264 T15699 T15697 arg1Of cells,KO
R12265 T15699 T15698 arg1Of cells,SP
R12266 T15702 T15703 arg2Of those,shown
R12267 T15703 T15704 arg1Of shown,in
R12268 T15705 T15706 arg1Of Figs.,3
R12269 T15705 T15707 arg1Of Figs.,and
R12270 T15707 T15704 arg2Of and,in
R12271 T15708 T15707 arg2Of 4,and
R12272 T15709 T15701 arg2Of are,whereas
R12273 T15709 T15703 modOf are,shown
R12274 T15710 T15694 arg2Of from,are
R12275 T15710 T15709 arg2Of from,are
R12276 T15715 T15710 arg2Of cells,from
R12277 T15715 T15711 arg1Of cells,total
R12278 T15715 T15712 arg1Of cells,Runx3
R12279 T15715 T15713 arg1Of cells,KO
R12280 T15715 T15714 arg1Of cells,CD8
R12451 T16231 T16229 arg1Of assay,FACS-based
R12452 T16231 T16230 arg1Of assay,cytotoxicity
R12453 T16233 T16232 arg1Of measure,To
R12454 T16234 T16233 arg2Of cytotoxicity,measure
R12455 T16239 T16233 arg1Of cells,measure
R12456 T16239 T16236 arg1Of cells,EL4
R12457 T16239 T16237 arg1Of cells,thymoma
R12458 T16239 T16238 arg1Of cells,target
R12459 T16239 T16240 arg1Of cells,were
R12460 T16239 T16241 arg2Of cells,loaded
R12461 T16241 T16232 modOf loaded,To
R12462 T16241 T16235 arg1Of loaded,","
R12463 T16241 T16240 arg2Of loaded,were
R12464 T16241 T16242 arg1Of loaded,with
R12465 T16241 T16252 arg1Of loaded,before
R12466 T16241 T16261 arg1Of loaded,at
R12467 T16243 T16244 arg1Of 0,or
R12468 T16244 T16246 arg1Of or,μM
R12469 T16245 T16244 arg2Of 1,or
R12470 T16248 T16242 arg2Of peptide,with
R12471 T16248 T16243 arg1Of peptide,0
R12472 T16248 T16245 arg1Of peptide,1
R12473 T16248 T16247 arg1Of peptide,Gp33
R12474 T16248 T16249 arg1Of peptide,for
R12475 T16251 T16249 arg2Of h,for
R12476 T16251 T16250 arg1Of h,2
R12477 T16255 T16252 arg2Of coincubation,before
R12478 T16255 T16253 arg1Of coincubation,a
R12479 T16255 T16254 arg1Of coincubation,2-h
R12480 T16255 T16256 arg1Of coincubation,with
R12481 T16260 T16256 arg2Of cells,with
R12482 T16260 T16257 arg1Of cells,P14
R12483 T16260 T16258 arg1Of cells,CD8+
R12484 T16260 T16259 arg1Of cells,T
R12485 T16264 T16261 arg2Of ratios,at
R12486 T16264 T16262 arg1Of ratios,the
R12487 T16264 T16263 arg1Of ratios,effector-to-target
R12488 T16264 T16265 arg2Of ratios,indicated
R12489 T16265 T16266 arg1Of indicated,in
R12490 T16268 T16266 arg2Of figures,in
R12491 T16268 T16267 arg1Of figures,the
R12492 T16268 T16269 arg1Of figures,in
R12493 T16272 T16269 arg2Of plates,in
R12494 T16272 T16270 arg1Of plates,96-well
R12495 T16272 T16271 arg1Of plates,round-bottom
R12496 T16276 T16273 arg2Of period,After
R12497 T16276 T16274 arg1Of period,the
R12498 T16276 T16275 arg1Of period,coincubation
R12499 T16278 T16279 arg1Of cells,were
R12500 T16278 T16280 arg2Of cells,stained
R12501 T16280 T16273 arg1Of stained,After
R12502 T16280 T16277 arg1Of stained,","
R12503 T16280 T16279 arg2Of stained,were
R12504 T16280 T16281 arg1Of stained,with
R12505 T16283 T16284 arg1Of V–FITC,and
R12506 T16284 T16281 arg2Of and,with
R12507 T16284 T16282 arg1Of and,Annexin
R12508 T16285 T16284 arg2Of anti-CD8–allophycocyanin,and
R12509 T16287 T16286 arg1Of analysis,Data
R12510 T16287 T16288 arg1Of analysis,was
R12511 T16287 T16289 arg2Of analysis,performed
R12512 T16289 T16288 arg2Of performed,was
R12513 T16289 T16290 arg1Of performed,with
R12514 T16289 T16293 arg1Of performed,(
R12515 T16289 T16299 arg1Of performed,;
R12516 T16292 T16290 arg2Of software,with
R12517 T16292 T16291 arg1Of software,FlowJo
R12518 T16295 T16293 arg2Of Star,(
R12519 T16295 T16294 arg1Of Star,Tree
R12520 T16295 T16296 arg1Of Star,","
R12521 T16297 T16296 arg2Of Inc.,","
R12522 T16298 T16293 arg3Of ),(
R12523 T16302 T16300 arg1Of cells,EL4
R12524 T16302 T16301 arg1Of cells,target
R12525 T16302 T16303 arg1Of cells,(
R12526 T16302 T16307 arg1Of cells,were
R12527 T16302 T16308 arg2Of cells,gated
R12528 T16305 T16303 arg2Of events,(
R12529 T16305 T16304 arg1Of events,CD8-negative
R12530 T16306 T16303 arg3Of ),(
R12531 T16308 T16307 arg2Of gated,were
R12532 T16308 T16310 arg1Of gated,and
R12533 T16310 T16299 arg2Of and,;
R12534 T16310 T16309 arg1Of and,","
R12535 T16312 T16311 arg1Of percentage,the
R12536 T16312 T16313 arg1Of percentage,of
R12537 T16312 T16318 arg1Of percentage,was
R12538 T16312 T16319 arg2Of percentage,determined
R12539 T16317 T16313 arg2Of cells,of
R12540 T16317 T16314 arg1Of cells,Annexin
R12541 T16317 T16315 arg1Of cells,V+
R12542 T16317 T16316 arg1Of cells,target
R12543 T16319 T16310 arg2Of determined,and
R12544 T16319 T16318 arg2Of determined,was
R12670 T16510 T16511 arg1Of Cytokine,and
R12671 T16512 T16511 arg2Of surface,and
R12672 T16514 T16510 arg1Of staining,Cytokine
R12673 T16514 T16512 arg1Of staining,surface
R12674 T16514 T16513 arg1Of staining,marker
R12679 T16520 T16521 arg1Of cells,were
R12680 T16520 T16522 arg2Of cells,restimulated
R12681 T16520 T16536 arg2Of cells,indicated
R12682 T16522 T16521 arg2Of restimulated,were
R12683 T16522 T16523 arg1Of restimulated,with
R12684 T16522 T16535 arg1Of restimulated,unless
R12685 T16522 T16543 arg1Of restimulated,and
R12686 T16524 T16525 arg1Of 10,nM
R12687 T16528 T16527 arg1Of 1,+
R12688 T16530 T16523 arg2Of ionomycin,with
R12689 T16530 T16524 arg1Of ionomycin,10
R12690 T16530 T16526 arg1Of ionomycin,PMA
R12691 T16530 T16528 arg1Of ionomycin,1
R12692 T16530 T16529 arg1Of ionomycin,μM
R12693 T16530 T16531 arg1Of ionomycin,for
R12694 T16533 T16531 arg2Of h,for
R12695 T16533 T16532 arg1Of h,6
R12696 T16533 T16534 arg1Of h,(
R12697 T16536 T16535 arg2Of indicated,unless
R12698 T16536 T16537 arg1Of indicated,otherwise
R12699 T16536 T16538 arg1Of indicated,in
R12700 T16540 T16538 arg2Of figures,in
R12701 T16540 T16539 arg1Of figures,the
R12702 T16540 T16541 arg1Of figures,)
R12703 T16543 T16515 modOf and,To
R12704 T16543 T16519 arg1Of and,","
R12705 T16543 T16542 arg1Of and,","
R12706 T16546 T16544 arg1Of stains,intracellular
R12707 T16546 T16545 arg1Of stains,cytokine
R12708 T16546 T16547 arg1Of stains,were
R12709 T16546 T16548 arg2Of stains,performed
R12710 T16548 T16543 arg2Of performed,and
R12711 T16548 T16547 arg2Of performed,were
R12712 T16548 T16549 arg1Of performed,as
R12713 T16551 T16549 arg2Of described,as
R12714 T16551 T16550 arg1Of described,previously
R12715 T16551 T16552 arg1Of described,(
R12716 T16553 T16552 arg2Of 28,(
R12717 T16554 T16552 arg3Of ),(
R12718 T16556 T16555 arg1Of detect,To
R12719 T16557 T16556 arg2Of expression,detect
R12720 T16557 T16558 arg1Of expression,of
R12721 T16560 T16558 arg2Of molecules,of
R12722 T16560 T16559 arg1Of molecules,surface
R12723 T16562 T16556 arg1Of cells,detect
R12724 T16562 T16563 arg1Of cells,were
R12725 T16562 T16564 arg2Of cells,washed
R12726 T16562 T16568 arg1Of cells,resuspended
R12727 T16562 T16602 arg2Of cells,incubated
R12728 T16562 T16613 arg1Of cells,washed
R12729 T16562 T16616 arg1Of cells,resuspended
R12730 T16564 T16563 arg2Of washed,were
R12731 T16564 T16565 arg1Of washed,in
R12732 T16564 T16567 arg1Of washed,","
R12733 T16566 T16565 arg2Of PBS,in
R12734 T16567 T16602 modOf ",",incubated
R12735 T16567 T16612 arg1Of ",",","
R12736 T16568 T16567 arg2Of resuspended,","
R12737 T16568 T16569 arg1Of resuspended,in
R12738 T16572 T16570 arg1Of buffer,FACS
R12739 T16572 T16571 arg1Of buffer,wash
R12740 T16572 T16573 arg1Of buffer,(
R12741 T16572 T16590 arg1Of buffer,containing
R12742 T16572 T16593 arg1Of buffer,indicated
R12743 T16574 T16575 arg1Of 3,%
R12744 T16576 T16573 arg2Of FBS,(
R12745 T16576 T16574 arg1Of FBS,3
R12746 T16576 T16577 arg1Of FBS,","
R12747 T16576 T16582 arg1Of FBS,","
R12748 T16576 T16586 arg1Of FBS,","
R12749 T16578 T16579 arg1Of 0.1,%
R12750 T16581 T16577 arg2Of azide,","
R12751 T16581 T16578 arg1Of azide,0.1
R12752 T16581 T16580 arg1Of azide,sodium
R12753 T16584 T16583 arg1Of mM,30
R12754 T16585 T16582 arg2Of Hepes,","
R12755 T16585 T16584 arg1Of Hepes,mM
R12756 T16588 T16586 arg2Of PBS,","
R12757 T16588 T16587 arg1Of PBS,1×
R12758 T16589 T16573 arg3Of ),(
R12759 T16592 T16590 arg2Of antibodies,containing
R12760 T16592 T16591 arg1Of antibodies,the
R12761 T16593 T16568 arg2Of indicated,resuspended
R12762 T16593 T16594 arg1Of indicated,in
R12763 T16593 T16597 arg1Of indicated,at
R12764 T16593 T16601 arg1Of indicated,","
R12765 T16596 T16594 arg2Of figures,in
R12766 T16596 T16595 arg1Of figures,the
R12767 T16599 T16598 arg1Of optimized,previously
R12768 T16600 T16597 arg2Of concentrations,at
R12769 T16600 T16599 arg1Of concentrations,optimized
R12770 T16602 T16603 arg1Of incubated,for
R12771 T16605 T16603 arg2Of min,for
R12772 T16605 T16604 arg1Of min,15
R12773 T16605 T16606 arg1Of min,at
R12774 T16608 T16606 arg2Of temperature,at
R12775 T16608 T16607 arg1Of temperature,room
R12776 T16608 T16609 arg1Of temperature,(
R12777 T16610 T16609 arg2Of RT,(
R12778 T16611 T16609 arg3Of ),(
R12779 T16612 T16615 arg1Of ",",and
R12780 T16613 T16612 arg2Of washed,","
R12781 T16615 T16555 modOf and,To
R12782 T16615 T16561 arg1Of and,","
R12783 T16615 T16614 arg1Of and,","
R12784 T16616 T16615 arg2Of resuspended,and
R12785 T16616 T16617 arg1Of resuspended,in
R12786 T16616 T16623 arg1Of resuspended,before
R12787 T16616 T16625 arg1Of resuspended,on
R12788 T16618 T16619 arg1Of 2,%
R12789 T16622 T16617 arg2Of solution,in
R12790 T16622 T16618 arg1Of solution,2
R12791 T16622 T16620 arg1Of solution,formaldehyde
R12792 T16622 T16621 arg1Of solution,fixative
R12793 T16624 T16623 arg2Of acquisition,before
R12794 T16627 T16625 arg2Of FACSCalibur,on
R12795 T16627 T16626 arg1Of FACSCalibur,a
R12796 T16627 T16628 arg1Of FACSCalibur,(
R12797 T16629 T16628 arg2Of BD,(
R12798 T16630 T16628 arg3Of ),(
R12928 T16786 T16785 arg1Of transduction,Retroviral
R12929 T16786 T16787 arg1Of transduction,of
R12930 T16791 T16787 arg2Of cells,of
R12931 T16791 T16788 arg1Of cells,primary
R12932 T16791 T16789 arg1Of cells,CD8+
R12933 T16791 T16790 arg1Of cells,T
R12935 T16794 T16792 arg2Of experiments,For
R12936 T16797 T16796 arg1Of supernatants,viral
R12937 T16797 T16798 arg1Of supernatants,were
R12938 T16797 T16799 arg2Of supernatants,generated
R12940 T16799 T16792 arg1Of generated,For
R12941 T16799 T16798 arg2Of generated,were
R12942 T16799 T16800 arg1Of generated,by
R12943 T16803 T16801 arg1Of transfection,calcium
R12944 T16803 T16802 arg1Of transfection,phosphate
R12945 T16803 T16804 arg1Of transfection,of
R12946 T16803 T16807 arg1Of transfection,and
R12947 T16806 T16804 arg2Of cells,of
R12948 T16806 T16805 arg1Of cells,Phoenix
R12949 T16807 T16800 arg2Of and,by
R12950 T16808 T16807 arg2Of concentration,and
R12951 T16808 T16809 arg1Of concentration,by
R12952 T16808 T16812 arg1Of concentration,at
R12953 T16811 T16809 arg2Of centrifugation,by
R12954 T16811 T16810 arg1Of centrifugation,overnight
R12955 T16814 T16812 arg2Of g,at
R12956 T16814 T16813 arg1Of g,"6,000"
R12957 T16817 T16815 arg2Of h,At
R12958 T16817 T16816 arg1Of h,∼42
R12959 T16817 T16818 arg1Of h,after
R12960 T16822 T16818 arg2Of activation,after
R12961 T16822 T16819 arg1Of activation,the
R12962 T16822 T16820 arg1Of activation,initial
R12963 T16822 T16821 arg1Of activation,TCR
R12964 T16822 T16823 arg1Of activation,of
R12965 T16822 T16828 arg1Of activation,per
R12966 T16827 T16823 arg2Of cells,of
R12967 T16827 T16824 arg1Of cells,106
R12968 T16827 T16825 arg1Of cells,CD8+
R12969 T16827 T16826 arg1Of cells,T
R12970 T16829 T16828 arg2Of well,per
R12971 T16829 T16830 arg1Of well,in
R12972 T16832 T16830 arg2Of plates,in
R12973 T16832 T16831 arg1Of plates,12-well
R12974 T16836 T16834 arg1Of media,the
R12975 T16836 T16835 arg1Of media,culture
R12976 T16836 T16837 arg1Of media,was
R12977 T16836 T16838 arg2Of media,removed
R12978 T16836 T16840 arg2Of media,replaced
R12979 T16838 T16839 arg1Of removed,and
R12980 T16839 T16815 arg1Of and,At
R12981 T16839 T16833 arg1Of and,","
R12982 T16839 T16837 arg2Of and,was
R12983 T16840 T16839 arg2Of replaced,and
R12984 T16840 T16841 arg1Of replaced,with
R12985 T16843 T16841 arg2Of media,with
R12986 T16843 T16842 arg1Of media,complete
R12987 T16843 T16844 arg2Of media,supplemented
R12988 T16844 T16845 arg1Of supplemented,with
R12989 T16848 T16845 arg2Of polybrene,with
R12990 T16848 T16846 arg1Of polybrene,8
R12991 T16848 T16847 arg1Of polybrene,μg/ml
R12992 T16848 T16849 arg1Of polybrene,containing
R12993 T16850 T16851 arg1Of fresh,plus
R12994 T16852 T16851 arg2Of concentrated,plus
R12995 T16853 T16849 arg2Of virus,containing
R12996 T16853 T16850 arg1Of virus,fresh
R12997 T16853 T16852 arg1Of virus,concentrated
R12998 T16855 T16854 arg1Of plates,The
R12999 T16855 T16856 arg1Of plates,were
R13000 T16855 T16857 arg2Of plates,centrifuged
R13001 T16855 T16867 arg1Of plates,returning
R13002 T16857 T16856 arg2Of centrifuged,were
R13003 T16857 T16858 arg1Of centrifuged,at
R13004 T16857 T16861 arg1Of centrifuged,for
R13005 T16857 T16864 arg1Of centrifuged,at
R13006 T16857 T16866 arg1Of centrifuged,before
R13007 T16860 T16858 arg2Of g,at
R13008 T16860 T16859 arg1Of g,700
R13009 T16863 T16861 arg2Of h,for
R13010 T16863 T16862 arg1Of h,1
R13011 T16865 T16864 arg2Of RT,at
R13012 T16867 T16866 arg2Of returning,before
R13013 T16867 T16868 arg1Of returning,to
R13014 T16867 T16870 arg1Of returning,for
R13015 T16869 T16868 arg2Of 37°C,to
R13016 T16874 T16870 arg2Of h,for
R13017 T16874 T16871 arg1Of h,an
R13018 T16874 T16872 arg1Of h,additional
R13019 T16874 T16873 arg1Of h,5
R13020 T16876 T16875 arg1Of constructs,Retroviral
R13021 T16876 T16877 arg1Of constructs,for
R13022 T16876 T16885 arg1Of constructs,were
R13023 T16878 T16879 arg1Of Eomes-VP16,and
R13024 T16879 T16877 arg2Of and,for
R13025 T16884 T16879 arg2Of vector,and
R13026 T16884 T16880 arg1Of vector,the
R13027 T16884 T16881 arg1Of vector,MIG
R13028 T16884 T16882 arg1Of vector,control
R13029 T16884 T16883 arg1Of vector,empty
R13030 T16887 T16885 arg2Of gift,were
R13031 T16887 T16886 arg1Of gift,a
R13032 T16887 T16888 arg1Of gift,from
R13033 T16890 T16888 arg2Of Reiner,from
R13034 T16890 T16889 arg1Of Reiner,S.L.
R13035 T16890 T16891 arg1Of Reiner,(
R13036 T16890 T16900 arg1Of Reiner,(
R13037 T16892 T16891 arg2Of University,(
R13038 T16892 T16893 arg1Of University,of
R13039 T16892 T16895 arg1Of University,","
R13040 T16894 T16893 arg2Of Pennsylvania,of
R13041 T16896 T16895 arg2Of Philadelphia,","
R13042 T16896 T16897 arg1Of Philadelphia,","
R13043 T16898 T16897 arg2Of PA,","
R13044 T16899 T16891 arg3Of ),(
R13045 T16901 T16900 arg2Of 8,(
R13046 T16902 T16900 arg3Of ),(
R13047 T16967 T16963 arg1Of analysis,ChIP
R13048 T16967 T16964 arg1Of analysis,and
R13049 T16967 T16965 arg1Of analysis,real-time
R13050 T16967 T16966 arg1Of analysis,PCR
R13051 T16968 T16969 arg1Of 20,×
R13052 T16970 T16969 arg2Of 106,×
R13053 T16973 T16968 arg1Of cells,20
R13054 T16973 T16970 arg1Of cells,106
R13055 T16973 T16971 arg1Of cells,CD8+
R13056 T16973 T16972 arg1Of cells,T
R13057 T16973 T16974 arg1Of cells,per
R13058 T16973 T16976 arg1Of cells,were
R13059 T16973 T16977 arg2Of cells,fixed
R13060 T16973 T17014 arg1Of cells,were
R13061 T16973 T17015 arg2Of cells,incubated
R13062 T16975 T16974 arg2Of immunoprecipitation,per
R13063 T16977 T16976 arg2Of fixed,were
R13064 T16977 T16978 arg1Of fixed,by
R13065 T16977 T17013 arg1Of fixed,and
R13066 T16979 T16978 arg2Of adding,by
R13067 T16982 T16979 arg2Of volume,adding
R13068 T16982 T16980 arg1Of volume,a
R13069 T16982 T16981 arg1Of volume,1/10th
R13070 T16982 T16983 arg1Of volume,of
R13071 T16982 T16986 arg1Of volume,(
R13072 T16982 T17007 arg1Of volume,to
R13073 T16985 T16983 arg2Of solution,of
R13074 T16985 T16984 arg1Of solution,fixation
R13075 T16987 T16988 arg1Of 11.1,%
R13076 T16989 T16987 arg1Of formaldehyde,11.1
R13077 T16989 T16990 arg1Of formaldehyde,","
R13078 T16990 T16994 arg1Of ",",","
R13079 T16991 T16992 arg1Of 100,mM
R13080 T16993 T16990 arg2Of NaCl,","
R13081 T16993 T16991 arg1Of NaCl,100
R13082 T16994 T16998 arg1Of ",",","
R13083 T16995 T16996 arg1Of 1,mM
R13084 T16997 T16994 arg2Of EDTA,","
R13085 T16997 T16995 arg1Of EDTA,1
R13086 T16998 T17002 arg1Of ",",","
R13087 T16999 T17000 arg1Of 0.5,mM
R13088 T17001 T16998 arg2Of EGTA,","
R13089 T17001 T16999 arg1Of EGTA,0.5
R13090 T17002 T16986 arg2Of ",",(
R13091 T17003 T17004 arg1Of 50,mM
R13092 T17005 T17002 arg2Of Hepes,","
R13093 T17005 T17003 arg1Of Hepes,50
R13094 T17006 T16986 arg3Of ),(
R13095 T17009 T17007 arg2Of volume,to
R13096 T17009 T17008 arg1Of volume,1
R13097 T17009 T17010 arg1Of volume,of
R13098 T17012 T17010 arg2Of media,of
R13099 T17012 T17011 arg1Of media,culture
R13100 T17015 T17013 arg2Of incubated,and
R13101 T17015 T17014 arg2Of incubated,were
R13102 T17015 T17016 arg1Of incubated,for
R13103 T17017 T17018 arg1Of 10,or
R13104 T17019 T17018 arg2Of 30,or
R13105 T17020 T17016 arg2Of min,for
R13106 T17020 T17017 arg1Of min,10
R13107 T17020 T17019 arg1Of min,30
R13108 T17020 T17021 arg1Of min,at
R13109 T17022 T17021 arg2Of RT,at
R13110 T17023 T17024 arg1Of Fixation,was
R13111 T17023 T17025 arg2Of Fixation,stopped
R13112 T17025 T17024 arg2Of stopped,was
R13113 T17025 T17026 arg1Of stopped,with
R13114 T17025 T17032 arg1Of stopped,for
R13115 T17027 T17028 arg1Of 120,mM
R13116 T17029 T17026 arg2Of glycine,with
R13117 T17029 T17027 arg1Of glycine,120
R13118 T17029 T17030 arg1Of glycine,on
R13119 T17031 T17030 arg2Of ice,on
R13120 T17034 T17032 arg2Of min,for
R13121 T17034 T17033 arg1Of min,5
R13122 T17036 T17035 arg2Of cells,Fixed
R13123 T17036 T17037 arg1Of cells,were
R13124 T17036 T17038 arg2Of cells,washed
R13125 T17038 T17037 arg2Of washed,were
R13126 T17039 T17040 arg1Of 2×,with
R13127 T17039 T17072 arg1Of 2×,and
R13128 T17042 T17040 arg2Of PBS,with
R13129 T17042 T17041 arg1Of PBS,cold
R13130 T17042 T17043 arg1Of PBS,","
R13131 T17044 T17043 arg2Of 1×,","
R13132 T17044 T17045 arg1Of 1×,with
R13133 T17047 T17045 arg2Of solution,with
R13134 T17047 T17046 arg1Of solution,cold
R13135 T17047 T17048 arg1Of solution,I
R13136 T17047 T17049 arg1Of solution,(
R13137 T17050 T17051 arg1Of 10,mM
R13138 T17052 T17050 arg1Of Tris,10
R13139 T17052 T17053 arg1Of Tris,[
R13140 T17052 T17057 arg1Of Tris,","
R13141 T17054 T17053 arg2Of pH,[
R13142 T17054 T17055 arg1Of pH,7.5
R13143 T17056 T17053 arg3Of ],[
R13144 T17057 T17061 arg1Of ",",","
R13145 T17058 T17059 arg1Of 10,mM
R13146 T17060 T17057 arg2Of EDTA,","
R13147 T17060 T17058 arg1Of EDTA,10
R13148 T17061 T17065 arg1Of ",",","
R13149 T17062 T17063 arg1Of 0.5,mM
R13150 T17064 T17061 arg2Of EGTA,","
R13151 T17064 T17062 arg1Of EGTA,0.5
R13152 T17065 T17049 arg2Of ",",(
R13153 T17066 T17067 arg1Of 1,%
R13154 T17069 T17065 arg2Of X-100,","
R13155 T17069 T17066 arg1Of X-100,1
R13156 T17069 T17068 arg1Of X-100,Triton
R13157 T17070 T17049 arg3Of ),(
R13158 T17072 T17038 arg3Of and,washed
R13159 T17072 T17071 arg1Of and,","
R13160 T17073 T17072 arg2Of 1×,and
R13161 T17073 T17074 arg1Of 1×,with
R13162 T17076 T17074 arg2Of solution,with
R13163 T17076 T17075 arg1Of solution,cold
R13164 T17076 T17077 arg1Of solution,II
R13165 T17076 T17078 arg1Of solution,(
R13166 T17079 T17080 arg1Of 10,mM
R13167 T17081 T17079 arg1Of Tris,10
R13168 T17081 T17082 arg1Of Tris,[
R13169 T17081 T17086 arg1Of Tris,","
R13170 T17083 T17082 arg2Of pH,[
R13171 T17083 T17084 arg1Of pH,7.5
R13172 T17085 T17082 arg3Of ],[
R13173 T17086 T17090 arg1Of ",",","
R13174 T17087 T17088 arg1Of 1,mM
R13175 T17089 T17086 arg2Of EDTA,","
R13176 T17089 T17087 arg1Of EDTA,1
R13177 T17090 T17094 arg1Of ",",","
R13178 T17091 T17092 arg1Of 0.5,mM
R13179 T17093 T17090 arg2Of EGTA,","
R13180 T17093 T17091 arg1Of EGTA,0.5
R13181 T17094 T17078 arg2Of ",",(
R13182 T17095 T17096 arg1Of 200,mM
R13183 T17097 T17094 arg2Of NaCl,","
R13184 T17097 T17095 arg1Of NaCl,200
R13185 T17098 T17078 arg3Of ),(
R13186 T17100 T17099 arg2Of washes,After
R13187 T17103 T17102 arg1Of pellets,cell
R13188 T17103 T17104 arg1Of pellets,were
R13189 T17103 T17105 arg2Of pellets,resuspended
R13190 T17105 T17099 arg1Of resuspended,After
R13191 T17105 T17101 arg1Of resuspended,","
R13192 T17105 T17104 arg2Of resuspended,were
R13193 T17105 T17106 arg1Of resuspended,at
R13194 T17105 T17111 arg1Of resuspended,in
R13195 T17105 T17147 arg1Of resuspended,and
R13196 T17107 T17108 arg1Of 40,×
R13197 T17109 T17108 arg2Of 106,×
R13198 T17110 T17106 arg2Of cells/ml,at
R13199 T17110 T17107 arg1Of cells/ml,40
R13200 T17110 T17109 arg1Of cells/ml,106
R13201 T17114 T17111 arg2Of buffer,in
R13202 T17114 T17112 arg1Of buffer,ChIP
R13203 T17114 T17113 arg1Of buffer,lysis
R13204 T17114 T17115 arg1Of buffer,(
R13205 T17118 T17116 arg1Of NaCl,150
R13206 T17118 T17117 arg1Of NaCl,mM
R13207 T17118 T17119 arg1Of NaCl,","
R13208 T17119 T17127 arg1Of ",",","
R13209 T17120 T17121 arg1Of 25,mM
R13210 T17122 T17119 arg2Of Tris,","
R13211 T17122 T17120 arg1Of Tris,25
R13212 T17122 T17123 arg1Of Tris,[
R13213 T17124 T17123 arg2Of pH,[
R13214 T17124 T17125 arg1Of pH,7.5
R13215 T17126 T17123 arg3Of ],[
R13216 T17127 T17132 arg1Of ",",","
R13217 T17128 T17129 arg1Of 1,%
R13218 T17131 T17127 arg2Of X-100,","
R13219 T17131 T17128 arg1Of X-100,1
R13220 T17131 T17130 arg1Of X-100,Triton
R13221 T17132 T17136 arg1Of ",",","
R13222 T17133 T17134 arg1Of 0.1,%
R13223 T17135 T17132 arg2Of SDS,","
R13224 T17135 T17133 arg1Of SDS,0.1
R13225 T17136 T17142 arg1Of ",",and
R13226 T17137 T17138 arg1Of 0.5,%
R13227 T17139 T17140 arg1Of deoxycholate,plus
R13228 T17140 T17136 arg2Of plus,","
R13229 T17140 T17137 arg1Of plus,0.5
R13230 T17141 T17140 arg2Of protease,plus
R13231 T17142 T17115 arg2Of and,(
R13232 T17144 T17142 arg2Of inhibitors,and
R13233 T17144 T17143 arg1Of inhibitors,phosphatase
R13234 T17145 T17115 arg3Of ),(
R13235 T17147 T17146 arg1Of and,","
R13236 T17148 T17149 arg1Of chromatin,was
R13237 T17148 T17150 arg2Of chromatin,sheared
R13238 T17150 T17147 arg2Of sheared,and
R13239 T17150 T17149 arg2Of sheared,was
R13240 T17150 T17151 arg1Of sheared,with
R13241 T17153 T17151 arg2Of sonicator,with
R13242 T17153 T17152 arg1Of sonicator,a
R13243 T17153 T17154 modOf sonicator,to
R13244 T17155 T17154 arg1Of yield,to
R13245 T17158 T17155 arg2Of fragments,yield
R13246 T17158 T17156 arg1Of fragments,0.5–1-kb
R13247 T17158 T17157 arg1Of fragments,DNA
R13248 T17160 T17168 arg1Of preclearing,and
R13249 T17163 T17160 arg2Of chromatin,preclearing
R13250 T17163 T17161 arg1Of chromatin,the
R13251 T17163 T17162 arg1Of chromatin,sheared
R13252 T17163 T17164 arg1Of chromatin,with
R13253 T17167 T17164 arg2Of beads,with
R13254 T17167 T17165 arg1Of beads,protein
R13255 T17167 T17166 arg1Of beads,A–sepharose
R13256 T17168 T17159 arg2Of and,After
R13257 T17169 T17168 arg2Of removing,and
R13258 T17169 T17172 arg1Of removing,as
R13259 T17171 T17169 arg2Of %,removing
R13260 T17171 T17170 arg1Of %,5
R13261 T17174 T17172 arg2Of chromatin,as
R13262 T17174 T17173 arg1Of chromatin,input
R13263 T17176 T17160 arg1Of immunoprecipitation,preclearing
R13264 T17176 T17169 arg1Of immunoprecipitation,removing
R13265 T17176 T17177 arg1Of immunoprecipitation,was
R13266 T17176 T17178 arg2Of immunoprecipitation,performed
R13267 T17178 T17159 arg1Of performed,After
R13268 T17178 T17175 arg1Of performed,","
R13269 T17178 T17177 arg2Of performed,was
R13270 T17178 T17179 arg1Of performed,by
R13271 T17180 T17179 arg2Of adding,by
R13272 T17183 T17180 arg2Of amounts,adding
R13273 T17183 T17181 arg2Of amounts,optimized
R13274 T17183 T17182 arg1Of amounts,antibody
R13275 T17183 T17184 arg1Of amounts,(
R13276 T17185 T17184 arg2Of per,(
R13277 T17190 T17185 arg2Of equivalents,per
R13278 T17190 T17186 arg1Of equivalents,20
R13279 T17190 T17187 arg1Of equivalents,×
R13280 T17190 T17188 arg1Of equivalents,106
R13281 T17190 T17189 arg1Of equivalents,cell
R13282 T17191 T17184 arg3Of :,(
R13283 T17194 T17192 arg1Of anti-Eomes,2.5
R13284 T17194 T17193 arg1Of anti-Eomes,μg
R13285 T17194 T17195 arg1Of anti-Eomes,","
R13286 T17195 T17202 arg1Of ",",","
R13287 T17195 T17203 arg2Of ",",followed
R13288 T17198 T17197 arg1Of 100,:
R13289 T17200 T17195 arg2Of anti-Runx3,","
R13290 T17200 T17196 arg1Of anti-Runx3,1
R13291 T17200 T17198 arg1Of anti-Runx3,100
R13292 T17200 T17199 arg1Of anti-Runx3,dilution
R13293 T17200 T17201 arg1Of anti-Runx3,)
R13294 T17203 T17209 arg1Of followed,;
R13295 T17206 T17203 arg1Of incubation,followed
R13296 T17206 T17204 arg2Of incubation,by
R13297 T17206 T17205 arg1Of incubation,overnight
R13298 T17206 T17207 arg1Of incubation,at
R13299 T17208 T17207 arg2Of 4°C,at
R13300 T17212 T17210 arg1Of beads,protein
R13301 T17212 T17211 arg1Of beads,A–sepharose
R13302 T17212 T17213 arg1Of beads,were
R13303 T17212 T17214 arg2Of beads,added
R13304 T17214 T17209 arg2Of added,;
R13305 T17214 T17213 arg2Of added,were
R13306 T17214 T17215 arg1Of added,for
R13307 T17219 T17215 arg2Of h,for
R13308 T17219 T17216 arg1Of h,the
R13309 T17219 T17217 arg1Of h,last
R13310 T17219 T17218 arg1Of h,3
R13311 T17219 T17220 arg1Of h,of
R13312 T17223 T17220 arg2Of period,of
R13313 T17223 T17221 arg1Of period,the
R13314 T17223 T17222 arg1Of period,incubation
R13315 T17224 T17225 arg1Of Beads,were
R13316 T17224 T17226 arg2Of Beads,washed
R13317 T17226 T17225 arg2Of washed,were
R13318 T17227 T17228 arg1Of 2×,with
R13319 T17227 T17260 arg1Of 2×,","
R13320 T17230 T17228 arg2Of buffer,with
R13321 T17230 T17229 arg1Of buffer,RIPA
R13322 T17230 T17231 arg1Of buffer,(
R13323 T17232 T17233 arg1Of 50,mM
R13324 T17234 T17232 arg1Of Tris,50
R13325 T17234 T17235 arg1Of Tris,[
R13326 T17234 T17239 arg1Of Tris,","
R13327 T17236 T17235 arg2Of pH,[
R13328 T17236 T17237 arg1Of pH,8
R13329 T17238 T17235 arg3Of ],[
R13330 T17239 T17243 arg1Of ",",","
R13331 T17240 T17241 arg1Of 150,mM
R13332 T17242 T17239 arg2Of NaCl,","
R13333 T17242 T17240 arg1Of NaCl,150
R13334 T17243 T17247 arg1Of ",",","
R13335 T17244 T17245 arg1Of 1,mM
R13336 T17246 T17243 arg2Of EDTA,","
R13337 T17246 T17244 arg1Of EDTA,1
R13338 T17247 T17231 arg2Of ",",(
R13339 T17247 T17251 arg1Of ",",","
R13340 T17248 T17249 arg1Of 1,%
R13341 T17250 T17247 arg2Of NP-40,","
R13342 T17250 T17248 arg1Of NP-40,1
R13343 T17252 T17253 arg1Of 0.1,%
R13344 T17254 T17251 arg2Of SDS,","
R13345 T17254 T17252 arg1Of SDS,0.1
R13346 T17254 T17255 arg1Of SDS,","
R13347 T17256 T17257 arg1Of 0.5,%
R13348 T17258 T17255 arg2Of deoxycholate,","
R13349 T17258 T17256 arg1Of deoxycholate,0.5
R13350 T17259 T17231 arg3Of ),(
R13351 T17260 T17292 arg1Of ",",and
R13352 T17261 T17260 arg2Of 1×,","
R13353 T17261 T17262 arg1Of 1×,with
R13354 T17265 T17262 arg2Of buffer,with
R13355 T17265 T17263 arg1Of buffer,high
R13356 T17265 T17264 arg1Of buffer,salt
R13357 T17265 T17266 arg1Of buffer,(
R13358 T17267 T17268 arg1Of 50,mM
R13359 T17269 T17267 arg1Of Tris,50
R13360 T17269 T17270 arg1Of Tris,[
R13361 T17269 T17274 arg1Of Tris,","
R13362 T17271 T17270 arg2Of pH,[
R13363 T17271 T17272 arg1Of pH,8
R13364 T17273 T17270 arg3Of ],[
R13365 T17274 T17278 arg1Of ",",","
R13366 T17275 T17276 arg1Of 500,mM
R13367 T17277 T17274 arg2Of NaCl,","
R13368 T17277 T17275 arg1Of NaCl,500
R13369 T17278 T17282 arg1Of ",",","
R13370 T17279 T17280 arg1Of 1,mM
R13371 T17281 T17278 arg2Of EDTA,","
R13372 T17281 T17279 arg1Of EDTA,1
R13373 T17282 T17266 arg2Of ",",(
R13374 T17282 T17286 arg1Of ",",","
R13375 T17283 T17284 arg1Of 1,%
R13376 T17285 T17282 arg2Of NP-40,","
R13377 T17285 T17283 arg1Of NP-40,1
R13378 T17287 T17288 arg1Of 0.1,%
R13379 T17289 T17286 arg2Of SDS,","
R13380 T17289 T17287 arg1Of SDS,0.1
R13381 T17290 T17266 arg3Of ),(
R13382 T17292 T17226 arg3Of and,washed
R13383 T17292 T17291 arg1Of and,","
R13384 T17293 T17292 arg2Of 1×,and
R13385 T17293 T17294 arg1Of 1×,with
R13386 T17296 T17294 arg2Of buffer,with
R13387 T17296 T17295 arg1Of buffer,TE
R13388 T17300 T17297 arg2Of wash,After
R13389 T17300 T17298 arg1Of wash,the
R13390 T17300 T17299 arg1Of wash,last
R13391 T17302 T17303 arg1Of DNA,was
R13392 T17302 T17304 arg2Of DNA,eluted
R13393 T17304 T17297 arg1Of eluted,After
R13394 T17304 T17301 arg1Of eluted,","
R13395 T17304 T17303 arg2Of eluted,was
R13396 T17304 T17305 arg1Of eluted,by
R13397 T17306 T17305 arg2Of resuspending,by
R13398 T17308 T17306 arg2Of beads,resuspending
R13399 T17308 T17307 arg1Of beads,the
R13400 T17308 T17309 arg1Of beads,in
R13401 T17311 T17309 arg2Of buffer,in
R13402 T17311 T17310 arg1Of buffer,elution
R13403 T17311 T17312 arg1Of buffer,(
R13404 T17313 T17314 arg1Of 1,%
R13405 T17315 T17312 arg2Of SDS,(
R13406 T17315 T17313 arg1Of SDS,1
R13407 T17315 T17316 arg1Of SDS,","
R13408 T17317 T17318 arg1Of 100,mM
R13409 T17319 T17316 arg2Of NaHCO3,","
R13410 T17319 T17317 arg1Of NaHCO3,100
R13411 T17320 T17312 arg3Of ),(
R13412 T17322 T17323 arg1Of input,and
R13413 T17323 T17321 arg1Of and,Both
R13414 T17323 T17326 arg1Of and,were
R13415 T17323 T17328 arg2Of and,treated
R13416 T17325 T17323 arg2Of chromatin,and
R13417 T17325 T17324 arg1Of chromatin,ChIP
R13418 T17328 T17326 arg2Of treated,were
R13419 T17328 T17327 arg1Of treated,then
R13420 T17328 T17329 arg1Of treated,with
R13421 T17328 T17337 arg1Of treated,for
R13422 T17328 T17342 arg1Of treated,","
R13423 T17328 T17343 modOf treated,followed
R13424 T17331 T17329 arg2Of A,with
R13425 T17331 T17330 arg1Of A,RNase
R13426 T17331 T17332 arg1Of A,(
R13427 T17335 T17332 arg2Of total,(
R13428 T17335 T17333 arg1Of total,5
R13429 T17335 T17334 arg1Of total,μg
R13430 T17336 T17332 arg3Of ),(
R13431 T17339 T17337 arg2Of h,for
R13432 T17339 T17338 arg1Of h,1
R13433 T17339 T17340 arg1Of h,at
R13434 T17341 T17340 arg2Of 37°C,at
R13435 T17346 T17343 arg1Of addition,followed
R13436 T17346 T17344 arg2Of addition,by
R13437 T17346 T17345 arg1Of addition,the
R13438 T17346 T17347 arg1Of addition,of
R13439 T17349 T17348 arg1Of K,proteinase
R13440 T17349 T17350 arg1Of K,(
R13441 T17349 T17355 arg1Of K,and
R13442 T17353 T17350 arg2Of total,(
R13443 T17353 T17351 arg1Of total,100
R13444 T17353 T17352 arg1Of total,μg
R13445 T17354 T17350 arg3Of ),(
R13446 T17355 T17347 arg2Of and,of
R13447 T17357 T17355 arg2Of incubation,and
R13448 T17357 T17356 arg1Of incubation,overnight
R13449 T17357 T17358 arg1Of incubation,at
R13450 T17357 T17360 modOf incubation,to
R13451 T17359 T17358 arg2Of 65°C,at
R13452 T17361 T17360 arg1Of reverse,to
R13453 T17362 T17361 arg2Of cross-linking,reverse
R13454 T17363 T17364 arg1Of DNA,was
R13455 T17363 T17366 arg2Of DNA,purified
R13456 T17363 T17384 arg1Of DNA,resuspended
R13457 T17366 T17367 arg1Of purified,with
R13458 T17366 T17377 arg1Of purified,according
R13459 T17366 T17383 arg1Of purified,and
R13460 T17369 T17367 arg2Of columns,with
R13461 T17369 T17368 arg1Of columns,QIAquick
R13462 T17369 T17370 arg1Of columns,(
R13463 T17373 T17370 arg2Of Kit,(
R13464 T17373 T17371 arg1Of Kit,Gel
R13465 T17373 T17372 arg1Of Kit,Extraction
R13466 T17373 T17374 arg1Of Kit,;
R13467 T17375 T17374 arg2Of QIAGEN,;
R13468 T17376 T17370 arg3Of ),(
R13469 T17378 T17377 arg2Of to,according
R13470 T17380 T17379 arg1Of manufacturer,the
R13471 T17380 T17381 arg2Of manufacturer,'s
R13472 T17382 T17378 arg2Of instructions,to
R13473 T17382 T17381 arg1Of instructions,'s
R13474 T17383 T17364 arg2Of and,was
R13475 T17383 T17365 arg1Of and,then
R13476 T17384 T17383 arg2Of resuspended,and
R13477 T17384 T17385 arg1Of resuspended,in
R13478 T17388 T17385 arg2Of volume,in
R13479 T17388 T17386 arg1Of volume,a
R13480 T17388 T17387 arg1Of volume,50-μl
R13481 T17392 T17389 arg2Of detection,For
R13482 T17392 T17390 arg1Of detection,real-time
R13483 T17392 T17391 arg1Of detection,PCR
R13484 T17392 T17393 arg1Of detection,of
R13485 T17395 T17393 arg2Of targets,of
R13486 T17395 T17394 arg1Of targets,immunoprecipitated
R13487 T17395 T17396 arg1Of targets,using
R13488 T17399 T17398 arg1Of Green,SYBR
R13489 T17401 T17396 arg2Of Kit,using
R13490 T17401 T17397 arg1Of Kit,the
R13491 T17401 T17399 arg1Of Kit,Green
R13492 T17401 T17400 arg1Of Kit,PCR
R13493 T17405 T17403 arg1Of curve,a
R13494 T17405 T17404 arg1Of curve,standard
R13495 T17405 T17406 arg1Of curve,was
R13496 T17405 T17407 arg2Of curve,obtained
R13497 T17407 T17406 arg2Of obtained,was
R13498 T17407 T17408 arg1Of obtained,with
R13499 T17407 T17418 arg1Of obtained,and
R13500 T17410 T17408 arg2Of dilutions,with
R13501 T17410 T17409 arg1Of dilutions,serial
R13502 T17410 T17411 arg1Of dilutions,of
R13503 T17413 T17411 arg2Of DNA,of
R13504 T17413 T17412 arg1Of DNA,input
R13505 T17413 T17414 arg1Of DNA,for
R13506 T17416 T17414 arg2Of sample,for
R13507 T17416 T17415 arg1Of sample,each
R13508 T17418 T17389 arg1Of and,For
R13509 T17418 T17402 arg1Of and,","
R13510 T17418 T17417 arg1Of and,","
R13511 T17422 T17419 arg1Of DNA,1
R13512 T17422 T17420 arg1Of DNA,μl
R13513 T17422 T17421 arg1Of DNA,ChIP
R13514 T17422 T17423 arg1Of DNA,was
R13515 T17422 T17424 arg2Of DNA,used
R13516 T17424 T17418 arg2Of used,and
R13517 T17424 T17423 arg2Of used,was
R13518 T17424 T17425 arg1Of used,per
R13519 T17427 T17425 arg2Of reaction,per
R13520 T17427 T17426 arg1Of reaction,PCR
R13521 T17427 T17428 arg1Of reaction,(
R13522 T17429 T17428 arg2Of performed,(
R13523 T17429 T17430 arg1Of performed,in
R13524 T17431 T17430 arg2Of duplicates,in
R13525 T17432 T17428 arg3Of ),(
R13526 T17434 T17433 arg1Of curves,Melt
R13527 T17434 T17435 arg1Of curves,and
R13528 T17435 T17438 arg1Of and,were
R13529 T17435 T17439 arg2Of and,analyzed
R13530 T17437 T17435 arg2Of gels,and
R13531 T17437 T17436 arg1Of gels,agarose
R13532 T17439 T17438 arg2Of analyzed,were
R13533 T17439 T17440 modOf analyzed,to
R13534 T17441 T17440 arg1Of ensure,to
R13535 T17442 T17441 arg2Of amplification,ensure
R13536 T17442 T17443 arg1Of amplification,of
R13537 T17446 T17443 arg2Of sequences,of
R13538 T17446 T17444 arg1Of sequences,specific
R13539 T17446 T17445 arg1Of sequences,target
R13540 T17447 T17458 arg1Of Refer,for
R13541 T17450 T17447 arg2Of S1,Refer
R13542 T17450 T17448 arg1Of S1,to
R13543 T17450 T17449 arg1Of S1,Table
R13544 T17450 T17451 arg1Of S1,(
R13545 T17452 T17451 arg2Of available,(
R13546 T17452 T17453 arg1Of available,at
R13547 T17454 T17453 arg2Of http,at
R13548 T17454 T17455 arg1Of http,:
R13549 T17456 T17455 arg2Of //www.jem.org/cgi/content/full/jem.20081242/DC1,:
R13550 T17457 T17451 arg3Of ),(
R13551 T17460 T17458 arg2Of list,for
R13552 T17460 T17459 arg1Of list,a
R13553 T17460 T17461 arg1Of list,of
R13554 T17463 T17461 arg2Of sets,of
R13555 T17463 T17462 arg1Of sets,primer
R13556 T17465 T17464 arg1Of data,The
R13557 T17465 T17466 arg1Of data,are
R13558 T17465 T17467 arg2Of data,presented
R13559 T17467 T17466 arg2Of presented,are
R13560 T17467 T17468 arg1Of presented,as
R13561 T17467 T17479 arg1Of presented,","
R13562 T17467 T17480 modOf presented,assuming
R13563 T17470 T17468 arg2Of number,as
R13564 T17470 T17469 arg1Of number,the
R13565 T17470 T17471 arg1Of number,of
R13566 T17474 T17471 arg2Of sequences,of
R13567 T17474 T17472 arg2Of sequences,immunoprecipitated
R13568 T17474 T17473 arg1Of sequences,target
R13569 T17474 T17475 arg1Of sequences,relative
R13570 T17475 T17476 arg1Of relative,to
R13571 T17478 T17476 arg2Of chromatin,to
R13572 T17478 T17477 arg1Of chromatin,input
R13573 T17482 T17480 arg2Of copies,assuming
R13574 T17482 T17481 arg1Of copies,two
R13575 T17482 T17483 arg1Of copies,of
R13576 T17482 T17486 arg1Of copies,per
R13577 T17485 T17483 arg2Of sequence,of
R13578 T17485 T17484 arg1Of sequence,target
R13579 T17487 T17486 arg2Of cell,per
R13580 T17487 T17488 arg1Of cell,equivalent
R13581 T17487 T17489 arg2Of cell,used
R13582 T17489 T17490 arg1Of used,for
R13583 T17492 T17490 arg2Of ChIP,for
R13584 T17492 T17491 arg1Of ChIP,the
R13874 T17860 T17856 arg2Of and,was
R13875 T17861 T17860 arg2Of transferred,and
R13876 T17861 T17862 arg1Of transferred,to
R13877 T17864 T17863 arg1Of charged,positively
R13878 T17866 T17862 arg2Of membranes,to
R13879 T17866 T17864 arg1Of membranes,charged
R13880 T17866 T17865 arg1Of membranes,nylon
R13881 T17866 T17867 arg1Of membranes,(
R13882 T17866 T17873 arg1Of membranes,","
R13883 T17866 T17874 arg1Of membranes,which
R13884 T17866 T17875 arg1Of membranes,was
R13885 T17866 T17876 arg2Of membranes,confirmed
R13886 T17868 T17869 arg1Of Hybond-N+,;
R13887 T17869 T17867 arg2Of ;,(
R13888 T17871 T17869 arg2Of Healthcare,;
R13889 T17871 T17870 arg1Of Healthcare,GE
R13890 T17872 T17867 arg3Of ),(
R13891 T17876 T17875 arg2Of confirmed,was
R13892 T17880 T17876 arg1Of staining,confirmed
R13893 T17880 T17877 arg2Of staining,by
R13894 T17880 T17878 arg1Of staining,ethidium
R13895 T17880 T17879 arg1Of staining,bromide
R13896 T17880 T17881 arg1Of staining,of
R13897 T17880 T17885 arg1Of staining,on
R13898 T17884 T17881 arg2Of species,of
R13899 T17884 T17882 arg1Of species,ribosomal
R13900 T17884 T17883 arg1Of species,RNA
R13901 T17887 T17885 arg2Of membrane,on
R13902 T17887 T17886 arg1Of membrane,the
R13903 T17888 T17889 arg1Of Membranes,were
R13904 T17888 T17890 arg2Of Membranes,hybridized
R13905 T17890 T17889 arg2Of hybridized,were
R13906 T17890 T17891 arg1Of hybridized,with
R13907 T17892 T17893 arg1Of 1,ng/ml
R13908 T17894 T17895 arg1Of α-,[
R13909 T17896 T17895 arg2Of 32P,[
R13910 T17897 T17895 arg3Of ],[
R13911 T17898 T17894 arg1Of dCTP–labeled,α-
R13912 T17902 T17891 arg2Of probe,with
R13913 T17902 T17892 arg1Of probe,1
R13914 T17902 T17898 arg1Of probe,dCTP–labeled
R13915 T17902 T17899 arg1Of probe,trichloroacetic
R13916 T17902 T17900 arg1Of probe,acid
R13917 T17902 T17901 arg1Of probe,precipitable
R13918 T17902 T17903 arg1Of probe,in
R13919 T17906 T17903 arg2Of buffer,in
R13920 T17906 T17904 arg1Of buffer,ExpressHyb
R13921 T17906 T17905 arg1Of buffer,hybridization
R13922 T17906 T17907 arg1Of buffer,(
R13923 T17909 T17907 arg2Of Laboratories,(
R13924 T17909 T17908 arg1Of Laboratories,Clontech
R13925 T17909 T17910 arg1Of Laboratories,","
R13926 T17911 T17910 arg2Of Inc.,","
R13927 T17912 T17907 arg3Of ),(
R13928 T17915 T17913 arg1Of probes,All
R13929 T17915 T17914 arg1Of probes,cDNA
R13930 T17915 T17916 arg1Of probes,were
R13931 T17915 T17917 arg2Of probes,confirmed
R13932 T17915 T17919 arg1Of probes,have
R13933 T17917 T17916 arg2Of confirmed,were
R13934 T17919 T17917 arg3Of have,confirmed
R13935 T17919 T17918 arg1Of have,to
R13936 T17919 T17924 arg1Of have,under
R13937 T17923 T17919 arg2Of specificity,have
R13938 T17923 T17920 arg1Of specificity,the
R13939 T17923 T17921 arg1Of specificity,appropriate
R13940 T17923 T17922 arg1Of specificity,single-copy
R13941 T17926 T17924 arg2Of conditions,under
R13942 T17926 T17925 arg1Of conditions,these
R13943 T17926 T17927 arg1Of conditions,using
R13944 T17931 T17927 arg2Of analysis,using
R13945 T17931 T17928 arg1Of analysis,genomic
R13946 T17931 T17929 arg1Of analysis,Southern
R13947 T17931 T17930 arg1Of analysis,blot
R13948 T17933 T17932 arg1Of intensities,Band
R13949 T17933 T17934 arg1Of intensities,were
R13950 T17933 T17935 arg2Of intensities,acquired
R13951 T17935 T17934 arg2Of acquired,were
R13952 T17935 T17936 arg1Of acquired,by
R13953 T17937 T17936 arg2Of phosphorimaging,by
R13954 T17938 T17937 arg2Of analysis,phosphorimaging
R13955 T17941 T17939 arg2Of analysis,For
R13956 T17941 T17940 arg1Of analysis,Western
R13957 T17945 T17943 arg1Of lysates,whole-cell
R13958 T17945 T17944 arg1Of lysates,protein
R13959 T17945 T17946 arg1Of lysates,were
R13960 T17945 T17947 arg2Of lysates,obtained
R13961 T17945 T17996 arg1Of lysates,resuspending
R13962 T17945 T18005 arg1Of lysates,incubating
R13963 T17947 T17939 arg1Of obtained,For
R13964 T17947 T17942 arg1Of obtained,","
R13965 T17947 T17946 arg2Of obtained,were
R13966 T17947 T17948 arg1Of obtained,from
R13967 T17947 T17952 arg1Of obtained,at
R13968 T17947 T17960 arg1Of obtained,during
R13969 T17947 T17995 arg1Of obtained,by
R13970 T17951 T17948 arg2Of cells,from
R13971 T17951 T17949 arg1Of cells,CD8+
R13972 T17951 T17950 arg1Of cells,T
R13973 T17954 T17952 arg2Of time,at
R13974 T17954 T17953 arg1Of time,the
R13975 T17955 T17956 arg1Of points,indicated
R13976 T17956 T17954 arg2Of indicated,time
R13977 T17956 T17957 arg1Of indicated,in
R13978 T17959 T17957 arg2Of figures,in
R13979 T17959 T17958 arg1Of figures,the
R13980 T17962 T17960 arg2Of expansion,during
R13981 T17962 T17961 arg1Of expansion,clonal
R13982 T17962 T17963 arg1Of expansion,in
R13983 T17962 T17967 arg1Of expansion,with
R13984 T17964 T17965 arg1Of 100,U/ml
R13985 T17966 T17963 arg2Of IL-2,in
R13986 T17966 T17964 arg1Of IL-2,100
R13987 T17969 T17967 arg2Of buffer,with
R13988 T17969 T17968 arg1Of buffer,lysis
R13989 T17969 T17970 arg1Of buffer,(
R13990 T17971 T17972 arg1Of 50,mM
R13991 T17973 T17971 arg1Of Tris,50
R13992 T17973 T17974 arg1Of Tris,[
R13993 T17973 T17978 arg1Of Tris,","
R13994 T17975 T17974 arg2Of pH,[
R13995 T17975 T17976 arg1Of pH,7.5
R13996 T17977 T17974 arg3Of ],[
R13997 T17978 T17970 arg2Of ",",(
R13998 T17979 T17980 arg1Of 150,mM
R13999 T17981 T17979 arg1Of NaCl,150
R14000 T17981 T17982 arg1Of NaCl,","
R14001 T17982 T17986 arg1Of ",",","
R14002 T17983 T17984 arg1Of 10,%
R14003 T17985 T17982 arg2Of glycerol,","
R14004 T17985 T17983 arg1Of glycerol,10
R14005 T17986 T17990 arg1Of ",",","
R14006 T17987 T17988 arg1Of 5,mM
R14007 T17989 T17986 arg2Of EDTA,","
R14008 T17989 T17987 arg1Of EDTA,5
R14009 T17990 T17978 arg2Of ",",","
R14010 T17991 T17992 arg1Of 1,%
R14011 T17993 T17990 arg2Of NP-40,","
R14012 T17993 T17991 arg1Of NP-40,1
R14013 T17994 T17970 arg3Of ),(
R14014 T17996 T17998 arg1Of resuspending,in
R14015 T17996 T18004 arg1Of resuspending,and
R14016 T17997 T17996 arg2Of samples,resuspending
R14017 T18000 T17998 arg2Of μl,in
R14018 T18000 T17999 arg1Of μl,10
R14019 T18000 T18001 arg1Of μl,per
R14020 T18003 T18001 arg2Of cells,per
R14021 T18003 T18002 arg1Of cells,106
R14022 T18004 T17995 arg2Of and,by
R14023 T18005 T18004 arg2Of incubating,and
R14024 T18005 T18006 arg1Of incubating,on
R14025 T18005 T18008 arg1Of incubating,for
R14026 T18005 T18011 arg1Of incubating,in
R14027 T18007 T18006 arg2Of ice,on
R14028 T18010 T18008 arg2Of min,for
R14029 T18010 T18009 arg1Of min,30
R14030 T18013 T18011 arg2Of presence,in
R14031 T18013 T18012 arg1Of presence,the
R14032 T18013 T18014 arg1Of presence,of
R14033 T18016 T18014 arg2Of inhibitors,of
R14034 T18016 T18015 arg1Of inhibitors,protease
R14035 T18018 T18017 arg1Of analysis,Immunoblot
R14036 T18018 T18019 arg1Of analysis,was
R14037 T18018 T18020 arg2Of analysis,performed
R14038 T18020 T18019 arg2Of performed,was
R14039 T18020 T18021 arg1Of performed,with
R14040 T18023 T18021 arg2Of antibodies,with
R14041 T18023 T18022 arg1Of antibodies,the
R14042 T18023 T18024 arg2Of antibodies,indicated
R14043 T18024 T18025 arg1Of indicated,in
R14044 T18024 T18028 arg1Of indicated,after
R14045 T18027 T18025 arg2Of figures,in
R14046 T18027 T18026 arg1Of figures,the
R14047 T18029 T18028 arg2Of SDS-PAGE,after
R14048 T18029 T18030 arg1Of SDS-PAGE,(
R14049 T18032 T18031 arg1Of μg,10–30
R14050 T18032 T18033 arg1Of μg,of
R14051 T18032 T18036 arg1Of μg,was
R14052 T18032 T18037 arg2Of μg,loaded
R14053 T18035 T18033 arg2Of protein,of
R14054 T18035 T18034 arg1Of protein,total
R14055 T18037 T18030 arg2Of loaded,(
R14056 T18037 T18036 arg2Of loaded,was
R14057 T18037 T18038 arg1Of loaded,per
R14058 T18039 T18038 arg2Of well,per
R14059 T18040 T18030 arg3Of ),(
R14060 T18041 T18042 arg1Of Quantification,of
R14061 T18041 T18045 arg1Of Quantification,was
R14062 T18041 T18046 arg2Of Quantification,performed
R14063 T18041 T18059 arg2Of Quantification,normalized
R14064 T18041 T18061 arg1Of Quantification,loading
R14065 T18044 T18042 arg2Of protein,of
R14066 T18044 T18043 arg2Of protein,detected
R14067 T18046 T18047 arg1Of performed,with
R14068 T18046 T18058 arg1Of performed,and
R14069 T18051 T18049 arg1Of Box,Intelligent
R14070 T18051 T18050 arg1Of Box,Dark
R14071 T18052 T18047 arg2Of unit,with
R14072 T18052 T18048 arg1Of unit,an
R14073 T18052 T18051 arg1Of unit,Box
R14074 T18052 T18053 arg1Of unit,(
R14075 T18054 T18053 arg2Of LAS-3000,(
R14076 T18054 T18055 arg1Of LAS-3000,;
R14077 T18056 T18055 arg2Of Fujifilm,;
R14078 T18057 T18053 arg3Of ),(
R14079 T18058 T18045 arg2Of and,was
R14080 T18059 T18058 arg2Of normalized,and
R14081 T18059 T18060 arg1Of normalized,for
R14082 T18061 T18060 arg2Of loading,for
R14083 T18061 T18062 arg1Of loading,with
R14084 T18064 T18062 arg2Of amount,with
R14085 T18064 T18063 arg1Of amount,the
R14086 T18064 T18065 arg1Of amount,of
R14087 T18067 T18065 arg2Of Pol-II,of
R14088 T18067 T18066 arg1Of Pol-II,RNA
R14089 T18067 T18068 arg2Of Pol-II,detected
R14090 T18068 T18069 arg1Of detected,in
R14091 T18071 T18069 arg2Of lane,in
R14092 T18071 T18070 arg1Of lane,each
R14158 T18192 T18190 arg1Of material,Online
R14159 T18192 T18191 arg1Of material,supplemental
R14160 T18194 T18193 arg1Of S1,Fig.
R14161 T18194 T18195 arg1Of S1,shows
R14162 T18197 T18195 arg2Of characterization,shows
R14163 T18197 T18196 arg1Of characterization,the
R14164 T18197 T18198 arg1Of characterization,of
R14165 T18202 T18198 arg2Of cells,of
R14166 T18202 T18199 arg1Of cells,peripheral
R14167 T18202 T18200 arg1Of cells,CD8+
R14168 T18202 T18201 arg1Of cells,T
R14169 T18202 T18203 arg1Of cells,from
R14170 T18205 T18203 arg2Of mice,from
R14171 T18205 T18204 arg1Of mice,Runx3−/−
R14172 T18207 T18206 arg1Of S2,Fig.
R14173 T18207 T18208 arg1Of S2,shows
R14174 T18211 T18208 arg2Of expression,shows
R14175 T18211 T18209 arg1Of expression,effector
R14176 T18211 T18210 arg1Of expression,protein
R14177 T18211 T18212 arg1Of expression,by
R14178 T18214 T18213 arg1Of WT,Runx3
R14179 T18214 T18215 arg1Of WT,and
R14180 T18215 T18212 arg2Of and,by
R14181 T18215 T18218 arg1Of and,at
R14182 T18217 T18215 arg2Of cells,and
R14183 T18217 T18216 arg1Of cells,KO
R14184 T18219 T18218 arg2Of day,at
R14185 T18219 T18220 arg1Of day,4
R14186 T18219 T18221 arg1Of day,of
R14187 T18223 T18222 arg1Of vitro,in
R14188 T18224 T18221 arg2Of culture,of
R14189 T18224 T18223 arg1Of culture,vitro
R14190 T18226 T18225 arg1Of sequences,Primer
R14191 T18226 T18227 arg2Of sequences,used
R14192 T18226 T18231 arg1Of sequences,are
R14193 T18226 T18232 arg2Of sequences,shown
R14194 T18227 T18228 arg1Of used,for
R14195 T18230 T18228 arg2Of experiments,for
R14196 T18230 T18229 arg1Of experiments,ChIP
R14197 T18232 T18231 arg2Of shown,are
R14198 T18232 T18233 arg1Of shown,in
R14199 T18235 T18233 arg2Of S1,in
R14200 T18235 T18234 arg1Of S1,Table
R14203 T18238 T18237 arg1Of material,supplemental
R14209 T18244 T18236 arg1Of //www.jem.org/cgi/content/full/jem.20081242/DC1,Online
R14496 T18624 T18625 arg1Of Kinetics,of
R14497 T18624 T18628 arg1Of Kinetics,during
R14498 T18627 T18625 arg2Of expression,of
R14499 T18627 T18626 arg1Of expression,gene
R14500 T18632 T18630 arg1Of differentiation.,T
R14501 T18632 T18631 arg1Of differentiation.,cell
R14502 T18632 T18633 arg1Of differentiation.,(
R14503 T18634 T18633 arg2Of A,(
R14504 T18635 T18633 arg3Of ),(
R14505 T18636 T18628 arg2Of Kinetics,during
R14506 T18636 T18629 arg1Of Kinetics,CD8+
R14507 T18636 T18632 arg1Of Kinetics,differentiation.
R14508 T18636 T18637 arg1Of Kinetics,of
R14509 T18638 T18639 arg1Of Prf1,","
R14510 T18639 T18641 arg1Of ",",","
R14511 T18640 T18639 arg2Of Gzmb,","
R14512 T18641 T18647 arg1Of ",",and
R14513 T18642 T18641 arg2Of Tbx21,","
R14514 T18642 T18643 arg1Of Tbx21,(
R14515 T18644 T18643 arg2Of T-bet,(
R14516 T18645 T18643 arg3Of ),(
R14517 T18647 T18637 arg2Of and,of
R14518 T18647 T18646 arg1Of and,","
R14519 T18647 T18651 arg1Of and,in
R14520 T18650 T18647 arg2Of expression,and
R14521 T18650 T18648 arg1Of expression,Eomes
R14522 T18650 T18649 arg1Of expression,mRNA
R14523 T18656 T18651 arg2Of cells,in
R14524 T18656 T18652 arg1Of cells,differentiating
R14525 T18656 T18653 arg1Of cells,P14
R14526 T18656 T18654 arg1Of cells,CD8+
R14527 T18656 T18655 arg1Of cells,T
R14528 T18656 T18657 arg2Of cells,analyzed
R14529 T18660 T18657 arg1Of blotting,analyzed
R14530 T18660 T18658 arg2Of blotting,by
R14531 T18660 T18659 arg1Of blotting,Northern
R14532 T18661 T18662 arg1Of RNA,from
R14533 T18661 T18667 arg1Of RNA,was
R14534 T18661 T18668 arg2Of RNA,used
R14535 T18666 T18662 arg2Of cells,from
R14536 T18666 T18663 arg1Of cells,day
R14537 T18666 T18664 arg1Of cells,7
R14538 T18666 T18665 arg1Of cells,Th1
R14539 T18668 T18667 arg2Of used,was
R14540 T18668 T18669 arg1Of used,as
R14541 T18671 T18669 arg2Of control,as
R14542 T18671 T18670 arg1Of control,a
R14543 T18672 T18673 arg1Of Sizes,of
R14544 T18672 T18676 arg1Of Sizes,are
R14545 T18672 T18677 arg2Of Sizes,indicated.
R14546 T18675 T18673 arg2Of transcripts,of
R14547 T18675 T18674 arg1Of transcripts,mRNA
R14548 T18677 T18676 arg2Of indicated.,are
R14549 T18679 T18678 arg2Of B,(
R14550 T18680 T18678 arg3Of ),(
R14551 T18681 T18677 arg3Of Quantification,indicated.
R14552 T18681 T18678 arg1Of Quantification,(
R14553 T18681 T18682 arg1Of Quantification,of
R14554 T18681 T18686 arg1Of Quantification,by
R14555 T18685 T18682 arg2Of amounts,of
R14556 T18685 T18683 arg1Of amounts,relative
R14557 T18685 T18684 arg1Of amounts,mRNA
R14558 T18688 T18687 arg1Of analysis.,phosphorimager
R14559 T18688 T18689 arg1Of analysis.,(
R14560 T18690 T18689 arg2Of C,(
R14561 T18691 T18689 arg3Of ),(
R14562 T18692 T18686 arg2Of Kinetics,by
R14563 T18692 T18688 arg1Of Kinetics,analysis.
R14564 T18692 T18693 arg1Of Kinetics,of
R14565 T18695 T18693 arg2Of expression,of
R14566 T18695 T18694 arg1Of expression,protein
R14567 T18695 T18696 arg1Of expression,in
R14568 T18701 T18696 arg2Of cells,in
R14569 T18701 T18697 arg1Of cells,differentiating
R14570 T18701 T18698 arg1Of cells,P14
R14571 T18701 T18699 arg1Of cells,CD8+
R14572 T18701 T18700 arg1Of cells,T
R14573 T18701 T18702 arg2Of cells,analyzed
R14574 T18704 T18702 arg1Of immunoblotting,analyzed
R14575 T18704 T18703 arg2Of immunoblotting,by
R14576 T18705 T18706 arg1Of Sizes,of
R14577 T18705 T18709 arg1Of Sizes,are
R14578 T18705 T18710 arg2Of Sizes,indicated.
R14579 T18708 T18706 arg2Of bands,of
R14580 T18708 T18707 arg1Of bands,protein
R14581 T18710 T18709 arg2Of indicated.,are
R14582 T18712 T18711 arg2Of D,(
R14583 T18713 T18711 arg3Of ),(
R14584 T18716 T18710 arg3Of amounts,indicated.
R14585 T18716 T18711 arg1Of amounts,(
R14586 T18716 T18714 arg1Of amounts,Relative
R14587 T18716 T18715 arg1Of amounts,protein
R14588 T18716 T18717 arg2Of amounts,quantified
R14589 T18716 T18727 arg1Of amounts,for
R14590 T18717 T18718 arg1Of quantified,from
R14591 T18723 T18722 arg2Of E,(
R14592 T18724 T18722 arg3Of ),(
R14593 T18726 T18718 arg2Of staining,from
R14594 T18726 T18719 arg1Of staining,the
R14595 T18726 T18720 arg1Of staining,Western
R14596 T18726 T18721 arg1Of staining,blots.
R14597 T18726 T18722 arg1Of staining,(
R14598 T18726 T18725 arg1Of staining,Intracellular
R14599 T18729 T18728 arg1Of B,granzyme
R14600 T18729 T18730 arg1Of B,","
R14601 T18730 T18733 arg1Of ",",and
R14602 T18731 T18730 arg2Of IFN-γ,","
R14603 T18733 T18727 arg2Of and,for
R14604 T18733 T18732 arg1Of and,","
R14605 T18734 T18733 arg2Of TNF,and
R14606 T18737 T18735 arg1Of staining,Granzyme
R14607 T18737 T18736 arg1Of staining,B
R14608 T18737 T18738 arg1Of staining,was
R14609 T18737 T18739 arg1Of staining,specific
R14610 T18739 T18738 arg2Of specific,was
R14611 T18739 T18741 arg1Of specific,to
R14612 T18741 T18740 arg1Of to,relative
R14613 T18744 T18741 arg2Of control,to
R14614 T18744 T18742 arg1Of control,an
R14615 T18744 T18743 arg1Of control,isotype
R14616 T18744 T18745 arg1Of control,(
R14617 T18747 T18745 arg2Of depicted,(
R14618 T18747 T18746 arg1Of depicted,not
R14619 T18748 T18745 arg3Of ),(
R14620 T18749 T18750 arg1Of Cells,were
R14621 T18749 T18751 arg2Of Cells,restimulated
R14622 T18751 T18750 arg2Of restimulated,were
R14623 T18751 T18752 arg1Of restimulated,with
R14624 T18753 T18754 arg1Of PMA,and
R14625 T18754 T18752 arg2Of and,with
R14626 T18754 T18756 arg1Of and,for
R14627 T18755 T18754 arg2Of ionomycin,and
R14628 T18758 T18757 arg1Of h.,4
R14629 T18758 T18759 arg1Of h.,(
R14630 T18760 T18759 arg2Of F,(
R14631 T18761 T18759 arg3Of ),(
R14632 T18762 T18758 arg1Of FACS-based,h.
R14633 T18763 T18756 arg2Of assay,for
R14634 T18763 T18762 arg1Of assay,FACS-based
R14635 T18763 T18764 modOf assay,to
R14636 T18765 T18764 arg1Of measure,to
R14637 T18765 T18773 arg1Of measure,against
R14638 T18767 T18765 arg2Of activity,measure
R14639 T18767 T18766 arg1Of activity,cytolytic
R14640 T18767 T18768 arg1Of activity,of
R14641 T18772 T18768 arg2Of cells,of
R14642 T18772 T18769 arg1Of cells,P14
R14643 T18772 T18770 arg1Of cells,CD8+
R14644 T18772 T18771 arg1Of cells,T
R14645 T18775 T18773 arg2Of targets,against
R14646 T18775 T18774 arg1Of targets,EL4
R14647 T18775 T18776 arg2Of targets,loaded
R14648 T18776 T18777 arg1Of loaded,with
R14649 T18778 T18779 arg1Of 0,(
R14650 T18778 T18782 arg1Of 0,or
R14651 T18780 T18779 arg2Of −,(
R14652 T18781 T18779 arg3Of ),(
R14653 T18782 T18777 arg2Of or,with
R14654 T18783 T18782 arg2Of 1,or
R14655 T18783 T18784 arg1Of 1,(
R14656 T18785 T18784 arg2Of +,(
R14657 T18786 T18784 arg3Of ),(
R14658 T18789 T18765 arg3Of peptide,measure
R14659 T18789 T18787 arg1Of peptide,μM
R14660 T18789 T18788 arg1Of peptide,Gp33
R14661 T18789 T18790 arg1Of peptide,(
R14662 T18792 T18790 arg2Of ratio,(
R14663 T18792 T18791 arg1Of ratio,effector-to-target
R14664 T18792 T18795 arg1Of ratio,:
R14665 T18795 T18793 arg1Of :,=
R14666 T18795 T18794 arg1Of :,5
R14667 T18795 T18796 arg1Of :,1
R14668 T18797 T18790 arg3Of ),(
R14669 T18798 T18799 arg1Of Percentage,of
R14670 T18798 T18807 arg1Of Percentage,in
R14671 T18798 T18817 arg1Of Percentage,was
R14672 T18798 T18818 arg2Of Percentage,determined
R14673 T18806 T18799 arg2Of cells,of
R14674 T18806 T18800 arg1Of cells,Annexin
R14675 T18806 T18801 arg1Of cells,V+
R14676 T18806 T18802 arg1Of cells,(
R14677 T18806 T18803 arg1Of cells,apoptotic
R14678 T18806 T18804 arg1Of cells,)
R14679 T18806 T18805 arg1Of cells,target
R14680 T18812 T18807 arg2Of population,in
R14681 T18812 T18808 arg1Of population,the
R14682 T18812 T18809 arg1Of population,CD8-negative
R14683 T18812 T18810 arg1Of population,EL4
R14684 T18812 T18811 arg1Of population,target
R14685 T18812 T18813 arg1Of population,(
R14686 T18815 T18813 arg2Of plots,(
R14687 T18815 T18814 arg1Of plots,dot
R14688 T18816 T18813 arg3Of ),(
R14689 T18818 T18817 arg2Of determined,was
R14690 T18818 T18819 arg1Of determined,(
R14691 T18820 T18819 arg2Of histograms,(
R14692 T18821 T18819 arg3Of ),(
R14693 T18823 T18822 arg1Of activity,Cytolytic
R14694 T18823 T18824 arg1Of activity,was
R14695 T18823 T18825 arg2Of activity,blocked
R14696 T18825 T18824 arg2Of blocked,was
R14697 T18825 T18826 arg1Of blocked,by
R14698 T18825 T18836 arg1Of blocked,","
R14699 T18825 T18837 modOf blocked,confirming
R14700 T18827 T18826 arg2Of incubation,by
R14701 T18827 T18828 arg1Of incubation,with
R14702 T18829 T18830 arg1Of 2,mM
R14703 T18831 T18828 arg2Of EGTA,with
R14704 T18831 T18829 arg1Of EGTA,2
R14705 T18831 T18832 arg1Of EGTA,(
R14706 T18834 T18832 arg2Of depicted,(
R14707 T18834 T18833 arg1Of depicted,not
R14708 T18835 T18832 arg3Of ),(
R14709 T18838 T18837 arg2Of involvement,confirming
R14710 T18838 T18839 arg1Of involvement,of
R14711 T18842 T18841 arg1Of exocytosis,granule
R14712 T18842 T18843 arg1Of exocytosis,(
R14713 T18845 T18843 arg2Of B,(
R14714 T18845 T18844 arg1Of B,perforin–granzyme
R14715 T18846 T18843 arg3Of ),(
R14716 T18847 T18839 arg2Of pathway,of
R14717 T18847 T18840 arg1Of pathway,the
R14718 T18847 T18842 arg1Of pathway,exocytosis
R14719 T18848 T18849 arg1Of Data,are
R14720 T18848 T18850 arg1Of Data,representative
R14721 T18850 T18849 arg2Of representative,are
R14722 T18850 T18851 arg1Of representative,of
R14723 T18854 T18852 arg1Of five,at
R14724 T18854 T18853 arg1Of five,least
R14725 T18856 T18855 arg2Of A–E,(
R14726 T18856 T18858 arg1Of A–E,or
R14727 T18857 T18855 arg3Of ),(
R14728 T18859 T18858 arg2Of three,or
R14729 T18861 T18860 arg2Of F,(
R14730 T18862 T18860 arg3Of ),(
R14731 T18864 T18851 arg2Of experiments,of
R14732 T18864 T18854 arg1Of experiments,five
R14733 T18864 T18855 arg1Of experiments,(
R14734 T18864 T18859 arg1Of experiments,three
R14735 T18864 T18860 arg1Of experiments,(
R14736 T18864 T18863 arg1Of experiments,independent
R15127 T19474 T19475 arg1Of Regulation,of
R15128 T19474 T19488 arg1Of Regulation,in
R15129 T19476 T19477 arg1Of perforin,","
R15130 T19477 T19481 arg1Of ",",and
R15131 T19479 T19477 arg2Of B,","
R15132 T19479 T19478 arg1Of B,granzyme
R15133 T19481 T19475 arg2Of and,of
R15134 T19481 T19480 arg1Of and,","
R15135 T19481 T19484 arg1Of and,by
R15136 T19483 T19481 arg2Of expression,and
R15137 T19483 T19482 arg1Of expression,IFN-γ
R15138 T19485 T19486 arg1Of T-bet,and
R15139 T19486 T19484 arg2Of and,by
R15140 T19487 T19486 arg2Of Eomes,and
R15141 T19489 T19488 arg2Of differentiating,in
R15142 T19489 T19496 arg1Of differentiating,by
R15143 T19490 T19491 arg1Of CTLs.,(
R15144 T19492 T19491 arg2Of A,(
R15145 T19493 T19491 arg3Of ),(
R15146 T19495 T19489 arg2Of expression,differentiating
R15147 T19495 T19490 arg1Of expression,CTLs.
R15148 T19495 T19494 arg1Of expression,IFN-γ
R15149 T19497 T19498 arg1Of WT,(
R15150 T19497 T19501 arg1Of WT,and
R15151 T19499 T19498 arg2Of Tbx21+/+,(
R15152 T19500 T19498 arg3Of ),(
R15153 T19501 T19496 arg2Of and,by
R15154 T19504 T19503 arg2Of Tbx21−/−,(
R15155 T19505 T19503 arg3Of ),(
R15156 T19507 T19501 arg2Of cells,and
R15157 T19507 T19502 arg1Of cells,T-bet–deficient
R15158 T19507 T19503 arg1Of cells,(
R15159 T19507 T19506 arg1Of cells,T
R15160 T19511 T19508 arg1Of cells,Naive
R15161 T19511 T19509 arg1Of cells,CD8+
R15162 T19511 T19510 arg1Of cells,T
R15163 T19511 T19513 arg1Of cells,or
R15164 T19513 T19512 arg1Of or,","
R15165 T19513 T19524 arg1Of or,were
R15166 T19513 T19525 arg2Of or,restimulated
R15167 T19514 T19513 arg2Of cells,or
R15168 T19514 T19515 arg2Of cells,activated
R15169 T19514 T19517 arg2Of cells,cultured
R15170 T19515 T19516 arg1Of activated,and
R15171 T19517 T19516 arg2Of cultured,and
R15172 T19517 T19518 arg1Of cultured,for
R15173 T19519 T19520 arg1Of 4,or
R15174 T19521 T19520 arg2Of 6,or
R15175 T19522 T19518 arg2Of d,for
R15176 T19522 T19519 arg1Of d,4
R15177 T19522 T19521 arg1Of d,6
R15178 T19525 T19523 arg1Of restimulated,","
R15179 T19525 T19524 arg2Of restimulated,were
R15180 T19525 T19526 arg1Of restimulated,with
R15181 T19525 T19530 arg1Of restimulated,for
R15182 T19525 T19534 arg1Of restimulated,and
R15183 T19527 T19528 arg1Of PMA,and
R15184 T19528 T19526 arg2Of and,with
R15185 T19529 T19528 arg2Of ionomycin,and
R15186 T19532 T19530 arg2Of h,for
R15187 T19532 T19531 arg1Of h,6
R15188 T19534 T19533 arg1Of and,","
R15189 T19536 T19535 arg1Of expression,IFN-γ
R15190 T19536 T19537 arg1Of expression,was
R15191 T19536 T19538 arg2Of expression,assessed
R15192 T19538 T19534 arg2Of assessed,and
R15193 T19538 T19537 arg2Of assessed,was
R15194 T19541 T19538 arg1Of staining,assessed
R15195 T19541 T19539 arg2Of staining,by
R15196 T19541 T19540 arg1Of staining,intracellular
R15197 T19542 T19543 arg1Of Numbers,show
R15198 T19545 T19543 arg2Of percentage,show
R15199 T19545 T19544 arg1Of percentage,the
R15200 T19545 T19546 arg1Of percentage,of
R15201 T19545 T19549 arg1Of percentage,(
R15202 T19548 T19546 arg2Of cells.,of
R15203 T19548 T19547 arg1Of cells.,IFN-γ+
R15204 T19550 T19549 arg2Of B,(
R15205 T19551 T19549 arg3Of ),(
R15206 T19554 T19543 arg3Of analysis,show
R15207 T19554 T19552 arg1Of analysis,Northern
R15208 T19554 T19553 arg1Of analysis,blot
R15209 T19554 T19555 arg1Of analysis,of
R15210 T19556 T19557 arg1Of Prf1,and
R15211 T19558 T19557 arg2Of GzmB,and
R15212 T19560 T19555 arg2Of expression,of
R15213 T19560 T19556 arg1Of expression,Prf1
R15214 T19560 T19558 arg1Of expression,GzmB
R15215 T19560 T19559 arg1Of expression,mRNA
R15216 T19560 T19561 arg1Of expression,in
R15217 T19562 T19563 arg1Of WT,or
R15218 T19564 T19563 arg2Of T-bet–deficient,or
R15219 T19567 T19561 arg2Of cells,in
R15220 T19567 T19562 arg1Of cells,WT
R15221 T19567 T19564 arg1Of cells,T-bet–deficient
R15222 T19567 T19565 arg1Of cells,CD8+
R15223 T19567 T19566 arg1Of cells,T
R15224 T19567 T19568 arg2Of cells,activated
R15225 T19567 T19572 arg1Of cells,uninfected
R15226 T19567 T19577 arg2Of cells,transduced
R15227 T19568 T19569 arg1Of activated,and
R15228 T19569 T19576 arg1Of and,or
R15229 T19572 T19569 arg2Of uninfected,and
R15230 T19572 T19570 arg1Of uninfected,either
R15231 T19572 T19571 arg1Of uninfected,left
R15232 T19572 T19573 arg1Of uninfected,(
R15233 T19574 T19573 arg2Of uninf,(
R15234 T19575 T19573 arg3Of ),(
R15235 T19577 T19576 arg2Of transduced,or
R15236 T19577 T19578 arg1Of transduced,with
R15237 T19579 T19578 arg2Of retroviruses,with
R15238 T19579 T19580 arg1Of retroviruses,expressing
R15239 T19581 T19582 arg1Of Eomes-VP16,(
R15240 T19581 T19585 arg1Of Eomes-VP16,or
R15241 T19583 T19582 arg2Of Eo-VP16,(
R15242 T19584 T19582 arg3Of ),(
R15243 T19585 T19580 arg2Of or,expressing
R15244 T19589 T19585 arg2Of cassette,or
R15245 T19589 T19586 arg1Of cassette,an
R15246 T19589 T19587 arg1Of cassette,empty
R15247 T19589 T19588 arg1Of cassette,IRES-GFP
R15248 T19589 T19590 arg1Of cassette,(
R15249 T19591 T19590 arg2Of GFP,(
R15250 T19592 T19590 arg3Of ),(
R15251 T19595 T19593 arg1Of RNA,Total
R15252 T19595 T19594 arg1Of RNA,cellular
R15253 T19595 T19596 arg1Of RNA,was
R15254 T19595 T19597 arg2Of RNA,analyzed
R15255 T19597 T19596 arg2Of analyzed,was
R15256 T19597 T19598 arg1Of analyzed,on
R15257 T19599 T19598 arg2Of day,on
R15258 T19599 T19600 arg1Of day,6
R15259 T19599 T19601 arg1Of day,of
R15260 T19602 T19601 arg2Of culture,of
R15261 T19604 T19603 arg1Of frequency,The
R15262 T19604 T19605 arg1Of frequency,of
R15263 T19604 T19608 arg1Of frequency,in
R15264 T19604 T19611 arg1Of frequency,was
R15265 T19604 T19612 arg1Of frequency,equivalent
R15266 T19607 T19605 arg2Of cells,of
R15267 T19607 T19606 arg2Of cells,transduced
R15268 T19610 T19608 arg2Of cultures,in
R15269 T19610 T19609 arg1Of cultures,the
R15270 T19612 T19611 arg2Of equivalent,was
R15271 T19612 T19613 arg1Of equivalent,for
R15272 T19615 T19613 arg2Of constructs,for
R15273 T19615 T19614 arg1Of constructs,both
R15274 T19617 T19618 arg1Of ∼65–70,%
R15275 T19620 T19616 arg2Of cells,(
R15276 T19620 T19617 arg1Of cells,∼65–70
R15277 T19620 T19619 arg1Of cells,GFP+
R15278 T19626 T19625 arg2Of (,.
R15279 T19627 T19625 arg3Of C,.
R15280 T19629 T19628 arg1Of m,) Granzy
R15281 T19629 T19630 arg1Of m,e B
R15282 T19630 T19625 arg1Of e B,.
R15283 T19632 T19630 arg2Of FN-γ expre,e B
R15284 T19632 T19631 arg1Of FN-γ expre,and I
R15285 T19634 T19635 arg1Of ion by T,bx2
R15286 T19636 T19635 arg2Of 1+/+ and,bx2
R15287 T19638 T19633 arg2Of bx21−,ss
R15288 T19638 T19634 arg1Of bx21−,ion by T
R15289 T19638 T19636 arg1Of bx21−,1+/+ and
R15290 T19638 T19637 arg1Of bx21−,T
R15291 T19638 T19639 arg2Of bx21−,/− T cel
R15292 T19638 T19643 arg1Of bx21−,mula
R15293 T19638 T19644 arg1Of bx21−,ted
R15294 T19638 T19645 arg1Of bx21−,cell
R15295 T19638 T19646 arg2Of bx21−,s that h
R15296 T19639 T19640 arg1Of /− T cel,ls
R15297 T19642 T19640 arg2Of resti,ls
R15298 T19642 T19641 arg2Of resti,analyzed in
R15299 T19646 T19644 arg2Of s that h,ted
R15300 T19646 T19645 arg2Of s that h,cell
R15301 T19646 T19647 arg1Of s that h,ad
R15302 T19649 T19648 arg1Of ee,b
R15303 T19649 T19650 arg1Of ee,n
R15304 T19651 T19650 arg2Of c,n
R15305 T19652 T19650 arg3Of u,n
R15306 T19654 T19647 arg2Of d for 5 d.,ad
R15307 T19654 T19649 arg1Of d for 5 d.,ee
R15308 T19654 T19653 arg1Of d for 5 d.,lture
R15309 T19656 T19655 arg2Of ) IFN,(D
R15310 T19656 T19657 arg2Of ) IFN,-γ product
R15311 T19657 T19658 arg1Of -γ product,ion
R15312 T19659 T19660 arg1Of by cell,s
R15313 T19660 T19658 arg2Of s ,ion
R15314 T19663 T19662 arg2Of ed ,c
R15315 T19664 T19662 arg3Of w,c
R15316 T19665 T19660 arg2Of ith Eo-VP16 ,s
R15317 T19665 T19661 arg1Of ith Eo-VP16 ,transdu
R15318 T19665 T19662 arg1Of ith Eo-VP16 ,c
R15319 T19665 T19666 arg1Of ith Eo-VP16 ,o
R15320 T19665 T19669 arg2Of ith Eo-VP16 ,ontrol (
R15321 T19667 T19666 arg2Of r ,o
R15322 T19668 T19666 arg3Of c,o
R15323 T19669 T19670 arg1Of ontrol (,GF
R15324 T19669 T19673 arg1Of ontrol (,etrov
R15325 T19671 T19670 arg2Of P) ,GF
R15326 T19671 T19672 arg1Of P) ,r
R15327 T19675 T19673 arg2Of r,etrov
R15328 T19675 T19674 arg1Of r,i
R15329 T19675 T19676 arg1Of r,us
R15330 T19677 T19676 arg2Of es (RV) measu,us
R15331 T19677 T19678 arg1Of es (RV) measu,red
R15332 T19679 T19680 arg1Of on ,day
R15333 T19680 T19678 arg2Of day,red
R15334 T19681 T19680 arg2Of 4 after 6,day
R15335 T19682 T19683 arg1Of Numbers,show
R15336 T19685 T19683 arg2Of percentage,show
R15337 T19685 T19684 arg1Of percentage,the
R15338 T19685 T19686 arg1Of percentage,of
R15339 T19689 T19686 arg2Of cells,of
R15340 T19689 T19687 arg1Of cells,GFP+
R15341 T19689 T19688 arg1Of cells,IFN-γ+
R15342 T19690 T19691 arg1Of Results,are
R15343 T19690 T19692 arg1Of Results,representative
R15344 T19692 T19691 arg2Of representative,are
R15345 T19692 T19693 arg1Of representative,of
R15346 T19694 T19695 arg1Of three,(
R15347 T19694 T19700 arg1Of three,or
R15348 T19696 T19697 arg1Of A,and
R15349 T19697 T19695 arg2Of and,(
R15350 T19698 T19697 arg2Of C,and
R15351 T19699 T19695 arg3Of ),(
R15352 T19700 T19702 arg1Of or,(
R15353 T19701 T19700 arg2Of two,or
R15354 T19703 T19704 arg1Of B,and
R15355 T19704 T19702 arg2Of and,(
R15356 T19705 T19704 arg2Of D,and
R15357 T19706 T19702 arg3Of ),(
R15358 T19708 T19693 arg2Of experiments,of
R15359 T19708 T19694 arg1Of experiments,three
R15360 T19708 T19701 arg1Of experiments,two
R15361 T19708 T19707 arg1Of experiments,independent
R16331 T21280 T21281 arg1Of Runx3,controls
R16332 T21282 T21281 arg2Of Eomes,controls
R16333 T21282 T21283 arg1Of Eomes,","
R16334 T21284 T21285 arg1Of perforin,","
R16335 T21285 T21289 arg1Of ",",and
R16336 T21287 T21285 arg2Of B,","
R16337 T21287 T21286 arg1Of B,granzyme
R16338 T21289 T21283 arg2Of and,","
R16339 T21289 T21288 arg1Of and,","
R16340 T21289 T21292 arg1Of and,in
R16341 T21291 T21289 arg2Of expression,and
R16342 T21291 T21290 arg1Of expression,IFN-γ
R16343 T21294 T21292 arg2Of CTLs,in
R16344 T21294 T21293 arg1Of CTLs,effector
R16345 T21295 T21296 arg1Of Runx3+/+,or
R16346 T21297 T21296 arg2Of Runx3−/−,or
R16347 T21300 T21295 arg1Of cells,Runx3+/+
R16348 T21300 T21297 arg1Of cells,Runx3−/−
R16349 T21300 T21298 arg1Of cells,CD8+
R16350 T21300 T21299 arg1Of cells,T
R16351 T21300 T21301 arg1Of cells,were
R16352 T21300 T21302 arg2Of cells,activated
R16353 T21300 T21304 arg2Of cells,transduced
R16354 T21302 T21303 arg1Of activated,and
R16355 T21303 T21301 arg2Of and,were
R16356 T21304 T21303 arg2Of transduced,and
R16357 T21304 T21305 arg1Of transduced,with
R16358 T21306 T21305 arg2Of retroviruses,with
R16359 T21306 T21307 arg1Of retroviruses,bearing
R16360 T21306 T21317 arg1Of retroviruses,encoding
R16361 T21307 T21315 arg1Of bearing,or
R16362 T21311 T21307 arg2Of cassette,bearing
R16363 T21311 T21308 arg1Of cassette,an
R16364 T21311 T21309 arg1Of cassette,empty
R16365 T21311 T21310 arg1Of cassette,IRES-GFP
R16366 T21311 T21312 arg1Of cassette,(
R16367 T21313 T21312 arg2Of GFP,(
R16368 T21314 T21312 arg3Of ),(
R16369 T21317 T21315 arg2Of encoding,or
R16370 T21317 T21316 arg1Of encoding,also
R16371 T21318 T21319 arg1Of Eomes-VP16,(
R16372 T21318 T21322 arg1Of Eomes-VP16,or
R16373 T21320 T21319 arg2Of Eo-VP16,(
R16374 T21321 T21319 arg3Of ),(
R16375 T21322 T21317 arg2Of or,encoding
R16376 T21323 T21322 arg2Of Myc-Runx3,or
R16377 T21323 T21324 arg1Of Myc-Runx3,(
R16378 T21325 T21324 arg2Of Runx3,(
R16379 T21326 T21324 arg3Of ),(
R16380 T21328 T21327 arg1Of frequency,The
R16381 T21328 T21329 arg1Of frequency,of
R16382 T21328 T21332 arg1Of frequency,in
R16383 T21328 T21335 arg1Of frequency,was
R16384 T21328 T21336 arg1Of frequency,equivalent
R16385 T21331 T21329 arg2Of cells,of
R16386 T21331 T21330 arg2Of cells,transduced
R16387 T21334 T21332 arg2Of cultures,in
R16388 T21334 T21333 arg1Of cultures,the
R16389 T21336 T21335 arg2Of equivalent,was
R16390 T21336 T21337 arg1Of equivalent,for
R16391 T21339 T21337 arg2Of constructs,for
R16392 T21339 T21338 arg1Of constructs,all
R16393 T21339 T21340 arg1Of constructs,(
R16394 T21341 T21342 arg1Of ∼75–90,%
R16395 T21344 T21340 arg2Of cells,(
R16396 T21344 T21341 arg1Of cells,∼75–90
R16397 T21344 T21343 arg1Of cells,GFP+
R16398 T21344 T21345 arg1Of cells,;
R16399 T21344 T21347 arg2Of cells,depicted
R16400 T21347 T21346 arg1Of depicted,not
R16401 T21348 T21340 arg3Of ),(
R16402 T21350 T21349 arg2Of (,.
R16403 T21351 T21349 arg3Of A,.
R16404 T21353 T21349 arg1Of in express,.
R16405 T21353 T21352 arg1Of in express,) Prote
R16406 T21353 T21354 arg1Of in express,io
R16407 T21353 T21361 arg1Of in express,(da
R16408 T21353 T21362 arg2Of in express,y 6) was
R16409 T21356 T21354 arg2Of -cell ex,io
R16410 T21356 T21355 arg1Of -cell ex,n in whole
R16411 T21356 T21357 arg1Of -cell ex,t
R16412 T21358 T21357 arg2Of rac,t
R16413 T21358 T21359 arg1Of rac,t
R16414 T21360 T21357 arg3Of s,t
R16415 T21362 T21335 arg3Of y 6) was,was
R16416 T21362 T21361 arg2Of y 6) was,(da
R16417 T21364 T21362 arg1Of alyzed by immu,y 6) was
R16418 T21364 T21363 arg2Of alyzed by immu,an
R16419 T21365 T21366 arg1Of Overexpression,of
R16420 T21365 T21368 arg1Of Overexpression,can
R16421 T21365 T21370 arg1Of Overexpression,be
R16422 T21365 T21371 arg2Of Overexpression,detected
R16423 T21367 T21366 arg2Of Eomes-VP16,of
R16424 T21371 T21368 arg2Of detected,can
R16425 T21371 T21369 arg1Of detected,not
R16426 T21371 T21370 arg2Of detected,be
R16427 T21371 T21372 arg1Of detected,with
R16428 T21371 T21376 arg1Of detected,","
R16429 T21371 T21377 arg1Of detected,as
R16430 T21374 T21373 arg1Of Eomes,the
R16431 T21375 T21372 arg2Of antibody,with
R16432 T21375 T21374 arg1Of antibody,Eomes
R16433 T21380 T21378 arg1Of epitope,the
R16434 T21380 T21379 arg1Of epitope,C-terminal
R16435 T21380 T21381 arg1Of epitope,is
R16436 T21380 T21382 arg1Of epitope,within
R16437 T21381 T21408 arg1Of is,and
R16438 T21382 T21381 arg2Of within,is
R16439 T21384 T21382 arg2Of region,within
R16440 T21384 T21383 arg1Of region,the
R16441 T21384 T21385 arg1Of region,that
R16442 T21384 T21386 arg1Of region,has
R16443 T21384 T21387 arg1Of region,been
R16444 T21384 T21388 arg2Of region,replaced
R16445 T21388 T21386 arg2Of replaced,has
R16446 T21388 T21387 arg2Of replaced,been
R16447 T21388 T21389 arg1Of replaced,with
R16448 T21388 T21403 arg1Of replaced,after
R16449 T21393 T21391 arg1Of domain.,VP16
R16450 T21393 T21392 arg1Of domain.,transactivation
R16451 T21393 T21394 arg1Of domain.,(
R16452 T21395 T21394 arg2Of B,(
R16453 T21396 T21394 arg3Of ),(
R16454 T21397 T21389 arg2Of Expression,with
R16455 T21397 T21390 arg1Of Expression,the
R16456 T21397 T21393 arg1Of Expression,domain.
R16457 T21397 T21398 arg1Of Expression,of
R16458 T21400 T21399 arg1Of B,granzyme
R16459 T21400 T21401 arg1Of B,and
R16460 T21401 T21398 arg2Of and,of
R16461 T21402 T21401 arg2Of IFN-γ,and
R16462 T21404 T21403 arg2Of culture,after
R16463 T21404 T21405 arg1Of culture,for
R16464 T21407 T21405 arg2Of d,for
R16465 T21407 T21406 arg1Of d,6
R16466 T21408 T21377 arg2Of and,as
R16467 T21409 T21410 arg1Of restimulation,for
R16468 T21409 T21417 arg1Of restimulation,was
R16469 T21409 T21418 arg2Of restimulation,determined
R16470 T21412 T21410 arg2Of h,for
R16471 T21412 T21411 arg1Of h,4
R16472 T21412 T21413 arg1Of h,with
R16473 T21414 T21415 arg1Of PMA,and
R16474 T21415 T21413 arg2Of and,with
R16475 T21416 T21415 arg2Of ionomycin,and
R16476 T21418 T21408 arg2Of determined,and
R16477 T21418 T21417 arg2Of determined,was
R16478 T21421 T21418 arg1Of staining,determined
R16479 T21421 T21419 arg2Of staining,by
R16480 T21421 T21420 arg1Of staining,intracellular
R16481 T21423 T21422 arg1Of percentage,The
R16482 T21423 T21424 arg1Of percentage,of
R16483 T21423 T21428 arg1Of percentage,is
R16484 T21423 T21429 arg2Of percentage,shown
R16485 T21426 T21425 arg1Of stained,positively
R16486 T21427 T21424 arg2Of cells,of
R16487 T21427 T21426 arg1Of cells,stained
R16488 T21429 T21428 arg2Of shown,is
R16489 T21429 T21430 arg1Of shown,above
R16490 T21429 T21433 arg1Of shown,;
R16491 T21432 T21430 arg2Of gate,above
R16492 T21432 T21431 arg1Of gate,the
R16493 T21437 T21434 arg1Of intensity,the
R16494 T21437 T21435 arg1Of intensity,mean
R16495 T21437 T21436 arg1Of intensity,fluorescence
R16496 T21437 T21438 arg1Of intensity,(
R16497 T21437 T21441 arg1Of intensity,of
R16498 T21437 T21449 arg1Of intensity,is
R16499 T21437 T21450 arg2Of intensity,shown
R16500 T21439 T21438 arg2Of MFI,(
R16501 T21440 T21438 arg3Of ),(
R16502 T21444 T21441 arg2Of staining,of
R16503 T21444 T21442 arg1Of staining,granzyme
R16504 T21444 T21443 arg1Of staining,B
R16505 T21444 T21445 arg1Of staining,for
R16506 T21448 T21445 arg2Of population,for
R16507 T21448 T21446 arg1Of population,the
R16508 T21448 T21447 arg1Of population,total
R16509 T21450 T21433 arg2Of shown,;
R16510 T21450 T21449 arg2Of shown,is
R16511 T21450 T21451 arg1Of shown,below
R16512 T21453 T21451 arg2Of gate,below
R16513 T21453 T21452 arg1Of gate,the
R16514 T21457 T21454 arg1Of lines,The
R16515 T21457 T21455 arg1Of lines,vertical
R16516 T21457 T21456 arg1Of lines,gray
R16517 T21457 T21458 arg1Of lines,indicate
R16518 T21460 T21458 arg2Of MFI,indicate
R16519 T21460 T21459 arg1Of MFI,the
R16520 T21460 T21461 arg1Of MFI,for
R16521 T21464 T21461 arg2Of cells,for
R16522 T21464 T21462 arg1Of cells,WT
R16523 T21464 T21463 arg1Of cells,GFP+
R16524 T21465 T21466 arg1Of Results,are
R16525 T21465 T21467 arg1Of Results,representative
R16526 T21467 T21466 arg2Of representative,are
R16527 T21467 T21468 arg1Of representative,of
R16528 T21471 T21469 arg1Of two,at
R16529 T21471 T21470 arg1Of two,least
R16530 T21473 T21468 arg2Of experiments.,of
R16531 T21473 T21471 arg1Of experiments.,two
R16532 T21473 T21472 arg1Of experiments.,independent
R16533 T21473 T21474 arg1Of experiments.,(
R16534 T21473 T21478 arg1Of experiments.,diagram
R16535 T21475 T21474 arg2Of C,(
R16536 T21476 T21474 arg3Of ),(
R16537 T21478 T21477 arg1Of diagram,Schematic
R16538 T21478 T21479 arg1Of diagram,of
R16539 T21482 T21479 arg2Of network,of
R16540 T21482 T21480 arg1Of network,the
R16541 T21482 T21481 arg1Of network,transcriptional
R16542 T21482 T21483 arg1Of network,involving
R16543 T21484 T21485 arg1Of Runx3,and
R16544 T21485 T21483 arg2Of and,involving
R16545 T21487 T21485 arg2Of factors,and
R16546 T21487 T21486 arg1Of factors,T-box
R16547 T21488 T21489 arg1Of T-bet,is
R16548 T21488 T21490 arg2Of T-bet,induced
R16549 T21488 T21495 arg1Of T-bet,is
R16550 T21488 T21496 arg1Of T-bet,essential
R16551 T21490 T21489 arg2Of induced,is
R16552 T21490 T21494 arg1Of induced,and
R16553 T21493 T21490 arg1Of signals,induced
R16554 T21493 T21491 arg2Of signals,by
R16555 T21493 T21492 arg1Of signals,TCR
R16556 T21495 T21494 arg2Of is,and
R16557 T21496 T21495 arg2Of essential,is
R16558 T21496 T21497 arg1Of essential,for
R16559 T21500 T21497 arg2Of expression,for
R16560 T21500 T21498 arg1Of expression,early
R16561 T21500 T21499 arg1Of expression,IFN-γ
R16562 T21501 T21502 arg1Of Runx3,is
R16563 T21501 T21503 arg1Of Runx3,present
R16564 T21501 T21510 arg1Of Runx3,represses
R16565 T21501 T21513 arg1Of Runx3,induces
R16566 T21502 T21509 arg1Of is,and
R16567 T21503 T21502 arg2Of present,is
R16568 T21503 T21504 arg1Of present,in
R16569 T21508 T21504 arg2Of cells,in
R16570 T21508 T21505 arg1Of cells,naive
R16571 T21508 T21506 arg1Of cells,CD8+
R16572 T21508 T21507 arg1Of cells,T
R16573 T21510 T21512 arg1Of represses,and
R16574 T21511 T21510 arg2Of Runx1,represses
R16575 T21512 T21509 arg2Of and,and
R16576 T21513 T21512 arg2Of induces,and
R16577 T21514 T21513 arg2Of Eomes,induces
R16578 T21514 T21515 arg1Of Eomes,","
R16579 T21516 T21517 arg1Of perforin,","
R16580 T21517 T21521 arg1Of ",",and
R16581 T21519 T21517 arg2Of B,","
R16582 T21519 T21518 arg1Of B,granzyme
R16583 T21521 T21515 arg2Of and,","
R16584 T21521 T21520 arg1Of and,","
R16585 T21523 T21521 arg2Of expression,and
R16586 T21523 T21522 arg1Of expression,IFN-γ
R16587 T21524 T21525 arg1Of Eomes,may
R16588 T21524 T21526 arg1Of Eomes,participate
R16589 T21526 T21525 arg2Of participate,may
R16590 T21526 T21527 arg1Of participate,in
R16591 T21526 T21533 arg1Of participate,whereas
R16592 T21528 T21527 arg2Of sustaining,in
R16593 T21531 T21528 arg2Of expression,sustaining
R16594 T21531 T21529 arg1Of expression,late
R16595 T21531 T21530 arg1Of expression,IFN-γ
R16596 T21533 T21532 arg1Of whereas,","
R16597 T21534 T21535 arg1Of Runx3,and
R16598 T21535 T21537 arg1Of and,(
R16599 T21535 T21542 arg1Of and,may
R16600 T21535 T21543 arg1Of and,cooperate
R16601 T21535 T21545 arg1Of and,activate
R16602 T21536 T21535 arg2Of Eomes,and
R16603 T21538 T21539 arg1Of but,not
R16604 T21540 T21537 arg2Of T-bet,(
R16605 T21540 T21538 arg1Of T-bet,but
R16606 T21541 T21537 arg3Of ),(
R16607 T21543 T21533 arg2Of cooperate,whereas
R16608 T21543 T21542 arg2Of cooperate,may
R16609 T21545 T21543 arg2Of activate,cooperate
R16610 T21545 T21544 arg1Of activate,to
R16611 T21547 T21545 arg2Of expression,activate
R16612 T21547 T21546 arg1Of expression,perforin
R16613 T21550 T21548 arg1Of line,The
R16614 T21550 T21549 arg1Of line,dotted
R16615 T21550 T21551 arg1Of line,indicates
R16616 T21554 T21551 arg2Of effect,indicates
R16617 T21554 T21552 arg1Of effect,the
R16618 T21554 T21553 arg1Of effect,partial
R16619 T21554 T21555 arg1Of effect,of
R16620 T21554 T21558 arg1Of effect,on
R16621 T21557 T21555 arg2Of deficiency,of
R16622 T21557 T21556 arg1Of deficiency,T-bet
R16623 T21560 T21559 arg1Of mRNA,Gzmb
R16624 T21560 T21561 arg1Of mRNA,but
R16625 T21561 T21558 arg2Of but,on
R16626 T21566 T21561 arg2Of expression,but
R16627 T21566 T21562 arg1Of expression,not
R16628 T21566 T21563 arg1Of expression,granzyme
R16629 T21566 T21564 arg1Of expression,B
R16630 T21566 T21565 arg1Of expression,protein
R12675 T16516 T16515 arg1Of assess,To
R12676 T16518 T16516 arg2Of production,assess
R12677 T16518 T16517 arg1Of production,cytokine
R12678 T16520 T16516 arg1Of cells,assess
R12934 T16794 T16793 arg1Of experiments,transduction
R12939 T16799 T16795 arg1Of generated,","
R13842 T17829 T17830 arg1Of Northern,and
R13843 T17831 T17830 arg2Of Western,and
R13844 T17833 T17829 arg1Of analyses,Northern
R13845 T17833 T17831 arg1Of analyses,Western
R13846 T17833 T17832 arg1Of analyses,blot
R13847 T17835 T17834 arg1Of isolation,RNA
R13848 T17835 T17844 arg2Of isolation,described
R13849 T17839 T17836 arg1Of analysis,and
R13850 T17839 T17837 arg1Of analysis,Northern
R13851 T17839 T17838 arg1Of analysis,blot
R13852 T17839 T17840 arg1Of analysis,was
R13853 T17839 T17841 arg2Of analysis,performed
R13854 T17841 T17840 arg2Of performed,was
R13855 T17841 T17842 arg1Of performed,as
R13856 T17842 T17843 arg1Of as,previously
R13857 T17844 T17840 modOf described,was
R13858 T17844 T17845 arg1Of described,(
R13859 T17846 T17845 arg2Of 29,(
R13860 T17847 T17845 arg3Of ),(
R13861 T17849 T17848 arg2Of brief,In
R13862 T17852 T17851 arg1Of μg,10
R13863 T17852 T17853 arg1Of μg,of
R13864 T17852 T17856 arg1Of μg,was
R13865 T17852 T17857 arg2Of μg,loaded
R13866 T17852 T17861 arg2Of μg,transferred
R13867 T17855 T17853 arg2Of RNA,of
R13868 T17855 T17854 arg1Of RNA,total
R13869 T17857 T17858 arg1Of loaded,per
R13870 T17857 T17860 arg1Of loaded,and
R13871 T17859 T17858 arg2Of lane,per
R13872 T17860 T17848 arg1Of and,In
R13873 T17860 T17850 arg1Of and,","
R14201 T18236 T18239 modOf Online,is
R14202 T18236 T18243 arg1Of Online,:
R14204 T18238 T18239 arg1Of material,is
R14205 T18238 T18240 arg1Of material,available
R14206 T18240 T18239 arg2Of available,is
R14207 T18240 T18241 arg1Of available,at
R14208 T18242 T18241 arg2Of http,at

bionlp-st-ge-2016-coref

Id Subject Object Predicate Lexical cue
T9873 18927-18934 Antecedent denotes Eo-VP16
T9874 20884-20889 Anaphor denotes their
T9875 19179-19184 Antecedent denotes Runx3
T9876 19189-19194 Antecedent denotes Eomes
T12595 21928-21930 Anaphor denotes It
T12596 21865-21870 Antecedent denotes Runx3
T12597 22091-22113 Anaphor denotes the latter three genes
T12598 21998-22008 Antecedent denotes granzyme B
T12599 22010-22018 Antecedent denotes perforin
T12600 22024-22029 Antecedent denotes IFN-γ
T12601 22628-22631 Anaphor denotes its
T12602 22542-22547 Antecedent denotes Runx3
T12603 23124-23129 Anaphor denotes which
T12604 23117-23122 Antecedent denotes Eomes
T12605 23620-23637 Anaphor denotes two other markers
T12606 23655-23659 Antecedent denotes Prf1
T12607 23664-23668 Antecedent denotes Gzmb
T1992 1326-1348 Anaphor denotes inflammatory cytokines
T1993 1357-1362 Antecedent denotes IFN-γ
T1994 1367-1370 Antecedent denotes TNF
T1995 2537-2541 Anaphor denotes they
T1996 2300-2305 Antecedent denotes T-bet
T1997 2310-2315 Antecedent denotes Eomes
T1998 2962-2965 Anaphor denotes its
T1999 2916-2921 Antecedent denotes Runx3
T445 561-588 Anaphor denotes T-box transcription factors
T446 511-516 Antecedent denotes T-bet
T447 539-551 Antecedent denotes Eomesodermin
T448 838-860 Anaphor denotes three cardinal markers
T449 890-895 Antecedent denotes IFN-γ
T450 897-905 Antecedent denotes perforin
T451 911-921 Antecedent denotes granzyme B
T9867 17323-17336 Anaphor denotes Both proteins
T9868 17236-17241 Antecedent denotes Eomes
T9869 17246-17251 Antecedent denotes Runx3
T9870 18807-18812 Anaphor denotes which
T9871 18800-18805 Antecedent denotes Runx1
T9872 19021-19023 Anaphor denotes it
T4255 5822-5825 Anaphor denotes its
T4256 5855-5863 Antecedent denotes perforin
T4257 6443-6456 Anaphor denotes this capacity
T4258 6373-6382 Antecedent denotes expressed
T5450 9287-9292 Anaphor denotes genes
T5451 9328-9332 Antecedent denotes Ifng
T5452 9338-9343 Anaphor denotes genes
T5453 9384-9388 Antecedent denotes Prf1
T5454 9393-9397 Antecedent denotes GzmB
T5455 9451-9478 Anaphor denotes these transcription factors
T5456 9233-9238 Antecedent denotes T-bet
T5457 9243-9248 Antecedent denotes Eomes
T5458 9910-9913 Anaphor denotes its
T5459 9755-9760 Antecedent denotes Eomes
T5460 10714-10719 Anaphor denotes which
T5461 10707-10712 Antecedent denotes T-bet
T6838 11475-11480 Anaphor denotes which
T6839 11468-11473 Antecedent denotes Eomes
R1706 T1992 T1993 boundBy inflammatory cytokines,IFN-γ
R1716 T1992 T1994 boundBy inflammatory cytokines,TNF
R9989 T12595 T12596 boundBy It,Runx3
R9990 T12597 T12598 boundBy the latter three genes,granzyme B
R9991 T12597 T12599 boundBy the latter three genes,perforin
R9992 T12597 T12600 boundBy the latter three genes,IFN-γ
R9993 T12601 T12602 boundBy its,Runx3
R9994 T12603 T12604 boundBy which,Eomes
R9995 T12605 T12606 boundBy two other markers,Prf1
R9996 T12605 T12607 boundBy two other markers,Gzmb
R1717 T1995 T1996 boundBy they,T-bet
R1725 T1995 T1997 boundBy they,Eomes
R1730 T1998 T1999 boundBy its,Runx3
R8041 T9867 T9868 boundBy Both proteins,Eomes
R8042 T9867 T9869 boundBy Both proteins,Runx3
R8045 T9870 T9871 boundBy which,Runx1
R8046 T9872 T9873 boundBy it,Eo-VP16
R8048 T9874 T9875 boundBy their,Runx3
R8049 T9874 T9876 boundBy their,Eomes
R468 T445 T446 boundBy T-box transcription factors,T-bet
R469 T445 T447 boundBy T-box transcription factors,Eomesodermin
R470 T448 T449 boundBy three cardinal markers,IFN-γ
R471 T448 T450 boundBy three cardinal markers,perforin
R472 T448 T451 boundBy three cardinal markers,granzyme B
R3444 T4255 T4256 boundBy its,perforin
R3445 T4257 T4258 boundBy this capacity,expressed
R4414 T5450 T5451 boundBy genes,Ifng
R4415 T5452 T5453 boundBy genes,Prf1
R4416 T5452 T5454 boundBy genes,GzmB
R4417 T5455 T5456 boundBy these transcription factors,T-bet
R4418 T5455 T5457 boundBy these transcription factors,Eomes
R4419 T5458 T5459 boundBy its,Eomes
R4420 T5460 T5461 boundBy which,T-bet
R5526 T6838 T6839 boundBy which,Eomes

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T88 0-5 JJ denotes Runx3
T89 6-9 CC denotes and
T90 10-15 JJ denotes T-box
T91 16-24 NNS denotes proteins
T92 25-34 VBP denotes cooperate
T93 35-37 TO denotes to
T94 38-47 VB denotes establish
T95 48-51 DT denotes the
T96 52-67 JJ denotes transcriptional
T97 68-75 NN denotes program
T98 76-78 IN denotes of
T99 79-87 NN denotes effector
T100 88-92 NNS denotes CTLs
T101 104-114 NNP denotes Activation
T102 115-117 IN denotes of
T103 118-123 JJ denotes naive
T104 124-127 NNP denotes CD8
T105 127-128 CD denotes +
T106 129-130 NNP denotes T
T107 131-136 NNS denotes cells
T108 137-141 IN denotes with
T109 142-149 NN denotes antigen
T110 150-157 VBZ denotes induces
T111 158-163 PRP$ denotes their
T112 164-179 NN denotes differentiation
T113 180-184 IN denotes into
T114 185-193 NN denotes effector
T115 194-203 JJ denotes cytolytic
T116 204-205 NNP denotes T
T117 206-217 NNS denotes lymphocytes
T118 218-219 -LRB- denotes (
T119 219-223 NNS denotes CTLs
T120 223-224 -RRB- denotes )
T121 224-225 . denotes .
T122 226-230 NNS denotes CTLs
T123 231-235 VBP denotes lyse
T124 236-244 JJ denotes infected
T125 245-247 CC denotes or
T126 248-256 JJ denotes aberrant
T127 257-263 NN denotes target
T128 264-269 NNS denotes cells
T129 270-272 IN denotes by
T130 273-283 NN denotes exocytosis
T131 284-286 IN denotes of
T132 287-292 JJ denotes lytic
T133 293-301 NNS denotes granules
T134 302-312 VBG denotes containing
T135 313-316 DT denotes the
T136 317-329 JJ denotes pore-forming
T137 330-337 NN denotes protein
T138 338-346 NN denotes perforin
T139 347-350 CC denotes and
T140 351-352 DT denotes a
T141 353-359 NN denotes family
T142 360-362 IN denotes of
T143 363-372 NNS denotes proteases
T144 373-379 VBD denotes termed
T145 380-389 NNS denotes granzymes
T146 389-390 . denotes .
T147 391-393 PRP denotes We
T148 394-398 VBP denotes show
T149 399-403 IN denotes that
T150 404-412 NN denotes effector
T151 413-416 NNP denotes CTL
T152 417-432 NN denotes differentiation
T153 433-439 VBZ denotes occurs
T154 440-442 IN denotes in
T155 443-446 CD denotes two
T156 447-457 JJ denotes sequential
T157 458-464 NNS denotes phases
T158 465-467 IN denotes in
T159 468-473 NN denotes vitro
T160 473-474 , denotes ,
T161 475-488 VBN denotes characterized
T162 489-491 IN denotes by
T163 492-497 JJ denotes early
T164 498-507 NN denotes induction
T165 508-510 IN denotes of
T166 511-516 NN denotes T-bet
T167 517-520 CC denotes and
T168 521-525 JJ denotes late
T169 526-535 NN denotes induction
T170 536-538 IN denotes of
T171 539-551 NNP denotes Eomesodermin
T172 552-553 -LRB- denotes (
T173 553-558 NNP denotes Eomes
T174 558-559 -RRB- denotes )
T175 559-560 , denotes ,
T176 561-566 JJ denotes T-box
T177 567-580 NN denotes transcription
T178 581-588 NNS denotes factors
T179 589-593 WDT denotes that
T180 594-602 VBP denotes regulate
T181 603-606 DT denotes the
T182 607-612 JJ denotes early
T183 613-616 CC denotes and
T184 617-621 JJ denotes late
T185 622-628 NNS denotes phases
T186 629-631 IN denotes of
T187 632-642 NN denotes interferon
T188 643-644 -LRB- denotes (
T189 644-647 NNP denotes IFN
T190 647-648 -RRB- denotes )
T191 649-650 VBP denotes γ
T192 651-661 NN denotes expression
T193 661-662 , denotes ,
T194 663-675 RB denotes respectively
T195 675-676 . denotes .
T196 677-679 IN denotes In
T197 680-688 NN denotes addition
T198 688-689 , denotes ,
T199 690-692 PRP denotes we
T200 693-704 VBP denotes demonstrate
T201 705-706 DT denotes a
T202 707-715 JJ denotes critical
T203 716-720 NN denotes role
T204 721-724 IN denotes for
T205 725-728 DT denotes the
T206 729-742 NN denotes transcription
T207 743-749 NN denotes factor
T208 750-755 NNP denotes Runx3
T209 756-758 IN denotes in
T210 759-762 NNP denotes CTL
T211 763-778 NN denotes differentiation
T212 778-779 . denotes .
T213 780-785 JJ denotes Runx3
T214 786-795 VBZ denotes regulates
T215 796-801 NNP denotes Eomes
T216 802-812 NN denotes expression
T217 813-815 RB denotes as
T218 816-820 RB denotes well
T219 821-823 IN denotes as
T220 824-834 NN denotes expression
T221 835-837 IN denotes of
T222 838-843 CD denotes three
T223 844-852 JJ denotes cardinal
T224 853-860 NNS denotes markers
T225 861-863 IN denotes of
T226 864-867 DT denotes the
T227 868-876 NN denotes effector
T228 877-880 NNP denotes CTL
T229 881-888 NN denotes program
T230 888-889 : denotes :
T231 890-895 NNP denotes IFN-γ
T232 895-896 , denotes ,
T233 897-905 NN denotes perforin
T234 905-906 , denotes ,
T235 907-910 CC denotes and
T236 911-919 NN denotes granzyme
T237 920-921 NN denotes B
T238 921-922 . denotes .
T239 923-926 PRP$ denotes Our
T240 927-931 NNS denotes data
T241 932-937 NN denotes point
T242 938-940 TO denotes to
T243 941-944 DT denotes the
T244 945-954 NN denotes existence
T245 955-957 IN denotes of
T246 958-960 DT denotes an
T247 961-970 JJ denotes elaborate
T248 971-986 JJ denotes transcriptional
T249 987-994 NN denotes network
T250 995-997 IN denotes in
T251 998-1003 WDT denotes which
T252 1004-1009 JJ denotes Runx3
T253 1010-1019 RB denotes initially
T254 1020-1027 VBZ denotes induces
T255 1028-1031 CC denotes and
T256 1032-1036 RB denotes then
T257 1037-1047 VBZ denotes cooperates
T258 1048-1052 IN denotes with
T259 1053-1058 JJ denotes T-box
T260 1059-1072 NN denotes transcription
T261 1073-1080 NNS denotes factors
T262 1081-1083 TO denotes to
T263 1084-1092 VB denotes regulate
T264 1093-1097 NN denotes gene
T265 1098-1111 NN denotes transcription
T266 1112-1114 IN denotes in
T267 1115-1130 VBG denotes differentiating
T268 1131-1135 NNS denotes CTLs
T269 1135-1136 . denotes .
T878 1138-1143 JJ denotes Naive
T879 1144-1147 NNP denotes CD8
T880 1147-1148 CD denotes +
T881 1149-1150 NNP denotes T
T882 1151-1156 NNS denotes cells
T883 1157-1170 VBP denotes differentiate
T884 1171-1175 IN denotes into
T885 1176-1184 NN denotes effector
T886 1185-1189 NNS denotes CTLs
T887 1190-1194 IN denotes with
T888 1195-1198 DT denotes the
T889 1199-1206 NN denotes ability
T890 1207-1209 TO denotes to
T891 1210-1214 VB denotes lyse
T892 1215-1230 JJ denotes antigen-bearing
T893 1231-1237 NN denotes target
T894 1238-1243 NNS denotes cells
T895 1244-1246 IN denotes by
T896 1247-1257 NN denotes exocytosis
T897 1258-1260 IN denotes of
T898 1261-1266 JJ denotes lytic
T899 1267-1275 NNS denotes granules
T900 1276-1286 VBG denotes containing
T901 1287-1295 NN denotes perforin
T902 1296-1299 CC denotes and
T903 1300-1309 NNS denotes granzymes
T904 1309-1310 , denotes ,
T905 1311-1314 CC denotes and
T906 1315-1317 TO denotes to
T907 1318-1325 VB denotes produce
T908 1326-1338 JJ denotes inflammatory
T909 1339-1348 NNS denotes cytokines
T910 1349-1353 JJ denotes such
T911 1354-1356 IN denotes as
T912 1357-1362 NNP denotes IFN-γ
T913 1363-1366 CC denotes and
T914 1367-1370 NNP denotes TNF
T915 1371-1375 IN denotes upon
T916 1376-1389 NN denotes restimulation
T917 1390-1397 IN denotes through
T918 1398-1401 DT denotes the
T919 1402-1405 NNP denotes TCR
T920 1406-1407 -LRB- denotes (
T921 1407-1411 CD denotes 1, 2
T922 1411-1412 -RRB- denotes )
T923 1412-1413 . denotes .
T924 1414-1416 IN denotes In
T925 1417-1421 NN denotes vivo
T926 1422-1433 NNS denotes experiments
T927 1434-1438 VBP denotes have
T928 1439-1449 VBN denotes elucidated
T929 1450-1454 JJ denotes many
T930 1455-1463 JJ denotes critical
T931 1464-1474 NNS denotes parameters
T932 1475-1484 VBG denotes governing
T933 1485-1488 DT denotes the
T934 1489-1500 NN denotes development
T935 1501-1504 CC denotes and
T936 1505-1514 NN denotes evolution
T937 1515-1517 IN denotes of
T938 1518-1525 JJ denotes primary
T939 1526-1529 NNP denotes CTL
T940 1530-1539 NNS denotes responses
T941 1540-1541 -LRB- denotes (
T942 1541-1545 CD denotes 3, 4
T943 1545-1546 -RRB- denotes )
T944 1546-1547 . denotes .
T945 1548-1550 IN denotes In
T946 1551-1555 DT denotes this
T947 1556-1561 NN denotes study
T948 1561-1562 , denotes ,
T949 1563-1565 PRP denotes we
T950 1566-1570 VBP denotes have
T951 1571-1575 VBN denotes used
T952 1576-1578 IN denotes in
T953 1579-1584 NN denotes vitro
T954 1585-1592 NNS denotes systems
T955 1593-1597 JJ denotes such
T956 1598-1600 IN denotes as
T957 1601-1606 DT denotes those
T958 1607-1616 VBN denotes developed
T959 1617-1619 TO denotes to
T960 1620-1625 VB denotes study
T961 1626-1629 NNP denotes CD4
T962 1629-1630 CD denotes +
T963 1631-1632 NNP denotes T
T964 1633-1637 NN denotes cell
T965 1638-1653 NN denotes differentiation
T966 1654-1656 TO denotes to
T967 1657-1663 VB denotes define
T968 1664-1667 DT denotes the
T969 1668-1677 JJ denotes molecular
T970 1678-1683 NN denotes basis
T971 1684-1686 IN denotes of
T972 1687-1695 NN denotes effector
T973 1696-1699 NNP denotes CTL
T974 1700-1715 NN denotes differentiation
T975 1716-1717 -LRB- denotes (
T976 1717-1721 CD denotes 5, 6
T977 1721-1722 -RRB- denotes )
T978 1722-1723 . denotes .
T979 1724-1727 DT denotes The
T980 1728-1733 NN denotes T-box
T981 1734-1747 NN denotes transcription
T982 1748-1755 NNS denotes factors
T983 1756-1768 NNP denotes Eomesodermin
T984 1769-1770 -LRB- denotes (
T985 1770-1775 NNP denotes Eomes
T986 1775-1776 -RRB- denotes )
T987 1777-1780 CC denotes and
T988 1781-1786 NNP denotes T-bet
T989 1787-1790 VBP denotes are
T990 1791-1797 VBN denotes needed
T991 1798-1801 IN denotes for
T992 1802-1811 JJ denotes important
T993 1812-1819 NNS denotes aspects
T994 1820-1822 IN denotes of
T995 1823-1831 NN denotes effector
T996 1832-1835 CC denotes and
T997 1836-1842 NN denotes memory
T998 1843-1846 NNP denotes CTL
T999 1847-1862 NN denotes differentiation
T1000 1863-1864 -LRB- denotes (
T1001 1864-1865 CD denotes 7
T1002 1865-1866 -RRB- denotes )
T1003 1866-1867 . denotes .
T1004 1868-1870 IN denotes In
T1005 1871-1881 JJ denotes uninfected
T1006 1882-1886 NNS denotes mice
T1007 1886-1887 , denotes ,
T1008 1888-1896 NN denotes compound
T1009 1897-1905 NN denotes deletion
T1010 1906-1908 IN denotes of
T1011 1909-1918 NNP denotes the Tbx21
T1012 1919-1920 -LRB- denotes (
T1013 1920-1928 VBG denotes encoding
T1014 1929-1934 NNP denotes T-bet
T1015 1934-1935 -RRB- denotes )
T1016 1936-1958 NNS denotes and eomesodermin genes
T1017 1959-1961 VBZ denotes is
T1018 1962-1972 VBN denotes associated
T1019 1973-1977 IN denotes with
T1020 1978-1979 DT denotes a
T1021 1980-1989 JJ denotes selective
T1022 1990-1994 NN denotes loss
T1023 1995-1997 IN denotes of
T1024 1998-2001 CD denotes CD8
T1025 2001-2002 CD denotes +
T1026 2003-2004 NNP denotes T
T1027 2005-2010 NNS denotes cells
T1028 2011-2015 IN denotes with
T1029 2016-2018 DT denotes an
T1030 2019-2025 NN denotes IL-2Rβ
T1031 2026-2030 JJ denotes high
T1032 2030-2031 , denotes ,
T1033 2032-2038 NN denotes memory
T1034 2039-2048 NN denotes phenotype
T1035 2049-2050 -LRB- denotes (
T1036 2050-2051 CD denotes 8
T1037 2051-2052 -RRB- denotes )
T1038 2052-2053 . denotes .
T1039 2054-2058 NNP denotes Mice
T1040 2059-2068 NN denotes deficient
T1041 2069-2072 IN denotes for
T1042 2073-2077 DT denotes both
T1043 2078-2083 NNP denotes T-bet
T1044 2084-2087 CC denotes and
T1045 2088-2093 NNP denotes Eomes
T1046 2094-2096 IN denotes in
T1047 2097-2098 NNP denotes T
T1048 2099-2104 NNS denotes cells
T1049 2105-2109 VBP denotes have
T1050 2110-2118 VBN denotes impaired
T1051 2119-2129 NN denotes expression
T1052 2130-2132 IN denotes of
T1053 2133-2142 JJ denotes cytolytic
T1054 2143-2152 NNS denotes mediators
T1055 2152-2153 , denotes ,
T1056 2154-2162 JJ denotes manifest
T1057 2163-2167 JJ denotes poor
T1058 2168-2177 JJ denotes cytolytic
T1059 2178-2186 NN denotes activity
T1060 2186-2187 , denotes ,
T1061 2188-2191 CC denotes and
T1062 2192-2196 VBP denotes fail
T1063 2197-2199 TO denotes to
T1064 2200-2207 VB denotes control
T1065 2208-2213 JJ denotes acute
T1066 2214-2225 JJ denotes lymphocytic
T1067 2226-2242 NN denotes choriomeningitis
T1068 2243-2248 NN denotes virus
T1069 2249-2258 NN denotes infection
T1070 2259-2260 -LRB- denotes (
T1071 2260-2261 CD denotes 9
T1072 2261-2262 -RRB- denotes )
T1073 2262-2263 . denotes .
T1074 2264-2276 RB denotes Nevertheless
T1075 2276-2277 , denotes ,
T1076 2278-2281 DT denotes the
T1077 2282-2290 JJ denotes specific
T1078 2291-2296 NNS denotes roles
T1079 2297-2299 IN denotes of
T1080 2300-2305 NN denotes T-bet
T1081 2306-2309 CC denotes and
T1082 2310-2315 NNS denotes Eomes
T1083 2316-2318 IN denotes in
T1084 2319-2325 JJ denotes clonal
T1085 2326-2335 NN denotes expansion
T1086 2336-2339 CC denotes and
T1087 2340-2343 NNP denotes CTL
T1088 2344-2359 NN denotes differentiation
T1089 2360-2364 VBP denotes have
T1090 2365-2368 RB denotes not
T1091 2369-2372 RB denotes yet
T1092 2373-2377 VBN denotes been
T1093 2378-2386 VBN denotes resolved
T1094 2386-2387 : denotes :
T1095 2388-2390 IN denotes in
T1096 2391-2401 JJ denotes particular
T1097 2401-2402 , denotes ,
T1098 2403-2405 PRP denotes it
T1099 2406-2408 VBZ denotes is
T1100 2409-2412 RB denotes not
T1101 2413-2418 VBN denotes known
T1102 2419-2426 IN denotes whether
T1103 2427-2432 DT denotes these
T1104 2433-2446 NN denotes transcription
T1105 2447-2454 NNS denotes factors
T1106 2455-2463 VBP denotes function
T1107 2464-2475 RB denotes redundantly
T1108 2476-2478 TO denotes to
T1109 2479-2486 VB denotes control
T1110 2487-2495 JJ denotes effector
T1111 2496-2499 NNP denotes CD8
T1112 2499-2500 CD denotes +
T1113 2501-2502 NNP denotes T
T1114 2503-2507 NN denotes cell
T1115 2508-2523 NN denotes differentiation
T1116 2523-2524 , denotes ,
T1117 2525-2528 CC denotes and
T1118 2529-2536 IN denotes whether
T1119 2537-2541 PRP denotes they
T1120 2542-2544 VBP denotes do
T1121 2545-2547 RB denotes so
T1122 2548-2556 RB denotes directly
T1123 2557-2559 IN denotes by
T1124 2560-2569 VBG denotes targeting
T1125 2570-2578 JJ denotes specific
T1126 2579-2587 NN denotes effector
T1127 2588-2596 NN denotes cytokine
T1128 2597-2600 CC denotes and
T1129 2601-2610 JJ denotes cytolytic
T1130 2611-2622 NNP denotes genes. Runx
T1131 2623-2631 NNS denotes proteins
T1132 2631-2632 , denotes ,
T1133 2633-2634 DT denotes a
T1134 2635-2641 NN denotes family
T1135 2642-2644 IN denotes of
T1136 2645-2650 CD denotes three
T1137 2651-2662 JJ denotes DNA-binding
T1138 2663-2676 NN denotes transcription
T1139 2677-2684 NNS denotes factors
T1140 2684-2685 , denotes ,
T1141 2686-2693 NN denotes control
T1142 2694-2703 NN denotes thymocyte
T1143 2704-2719 NN denotes differentiation
T1144 2720-2723 CC denotes and
T1145 2724-2727 DT denotes the
T1146 2728-2735 NNP denotes CD4/CD8
T1147 2736-2743 NN denotes lineage
T1148 2744-2752 NN denotes decision
T1149 2753-2754 -LRB- denotes (
T1150 2754-2756 CD denotes 10
T1151 2757-2759 CD denotes 13
T1152 2759-2760 -RRB- denotes )
T1153 2760-2761 . denotes .
T1154 2762-2767 JJ denotes Runx3
T1155 2768-2771 CC denotes and
T1156 2772-2780 JJ denotes perforin
T1157 2781-2785 NNP denotes mRNA
T1158 2786-2789 VBP denotes are
T1159 2790-2799 VBN denotes expressed
T1160 2800-2802 IN denotes by
T1161 2803-2818 JJ denotes double-positive
T1162 2819-2820 -LRB- denotes (
T1163 2820-2822 NNP denotes DP
T1164 2822-2823 -RRB- denotes )
T1165 2824-2834 NNS denotes thymocytes
T1166 2835-2838 CC denotes and
T1167 2839-2842 CD denotes CD8
T1168 2842-2843 CD denotes +
T1169 2844-2859 JJ denotes single-positive
T1170 2860-2861 -LRB- denotes (
T1171 2861-2863 NNP denotes SP
T1172 2863-2864 -RRB- denotes )
T1173 2865-2875 NNS denotes thymocytes
T1174 2876-2879 CC denotes but
T1175 2880-2883 RB denotes not
T1176 2884-2886 IN denotes in
T1177 2887-2890 CD denotes CD4
T1178 2890-2891 CD denotes +
T1179 2892-2894 NNP denotes SP
T1180 2895-2900 NNS denotes cells
T1181 2901-2902 -LRB- denotes (
T1182 2902-2904 CD denotes 14
T1183 2904-2905 -RRB- denotes )
T1184 2905-2906 . denotes .
T1185 2907-2915 IN denotes Although
T1186 2916-2921 NNP denotes Runx3
T1187 2922-2924 VBZ denotes is
T1188 2925-2928 RB denotes not
T1189 2929-2938 VBN denotes expressed
T1190 2939-2941 IN denotes in
T1191 2942-2947 JJ denotes naive
T1192 2948-2951 NNP denotes CD4
T1193 2951-2952 CD denotes +
T1194 2953-2954 NNP denotes T
T1195 2955-2960 NNS denotes cells
T1196 2960-2961 , denotes ,
T1197 2962-2965 PRP$ denotes its
T1198 2966-2976 NN denotes expression
T1199 2977-2979 VBZ denotes is
T1200 2980-2992 JJ denotes up-regulated
T1201 2993-2999 IN denotes during
T1202 3000-3003 NNP denotes Th1
T1203 3004-3008 NN denotes cell
T1204 3009-3024 NN denotes differentiation
T1205 3024-3025 , denotes ,
T1206 3026-3029 CC denotes and
T1207 3030-3035 JJ denotes Runx3
T1208 3036-3046 NNS denotes influences
T1209 3047-3050 JJ denotes Th1
T1210 3051-3055 NN denotes cell
T1211 3056-3071 NN denotes differentiation
T1212 3072-3075 CC denotes and
T1213 3076-3084 NN denotes function
T1214 3085-3092 IN denotes through
T1215 3093-3099 JJ denotes direct
T1216 3100-3110 NN denotes regulation
T1217 3111-3113 IN denotes of
T1218 3114-3139 JJ denotes the Il4 and Ifng cytokine
T1219 3140-3145 NNS denotes genes
T1220 3146-3147 -LRB- denotes (
T1221 3147-3153 CD denotes 15, 16
T1222 3153-3154 -RRB- denotes )
T1223 3154-3155 . denotes .
T1224 3156-3158 IN denotes In
T1225 3159-3167 NN denotes contrast
T1226 3167-3168 , denotes ,
T1227 3169-3172 DT denotes all
T1228 3173-3178 CD denotes three
T1229 3179-3183 NNP denotes Runx
T1230 3184-3192 NNS denotes proteins
T1231 3193-3196 VBP denotes are
T1232 3197-3206 VBN denotes expressed
T1233 3207-3209 IN denotes in
T1234 3210-3216 JJ denotes mature
T1235 3217-3220 NNP denotes CD8
T1236 3220-3221 CD denotes +
T1237 3222-3223 NNP denotes T
T1238 3224-3229 NNS denotes cells
T1239 3230-3231 -LRB- denotes (
T1240 3231-3237 CD denotes 10, 12
T1241 3237-3238 -RRB- denotes )
T1242 3238-3239 , denotes ,
T1243 3240-3243 CC denotes and
T1244 3244-3259 JJ denotes Runx3-deficient
T1245 3260-3263 CD denotes CD8
T1246 3263-3264 CD denotes +
T1247 3265-3266 NNP denotes T
T1248 3267-3272 NNS denotes cells
T1249 3273-3277 VBP denotes show
T1250 3278-3285 JJ denotes reduced
T1251 3286-3295 JJ denotes cytolytic
T1252 3296-3304 NN denotes activity
T1253 3305-3306 -LRB- denotes (
T1254 3306-3312 CD denotes 12, 13
T1255 3312-3313 -RRB- denotes )
T1256 3313-3314 . denotes .
T1257 3315-3317 PRP denotes We
T1258 3318-3327 RB denotes therefore
T1259 3328-3334 VBD denotes tested
T1260 3335-3342 IN denotes whether
T1261 3343-3348 JJ denotes Runx3
T1262 3349-3359 VBN denotes influenced
T1263 3360-3369 JJ denotes cytolytic
T1264 3370-3371 NNP denotes T
T1265 3372-3376 NN denotes cell
T1266 3377-3396 NN denotes differentiation. In
T1267 3397-3401 DT denotes this
T1268 3402-3408 NN denotes report
T1269 3408-3409 , denotes ,
T1270 3410-3412 PRP denotes we
T1271 3413-3417 VBP denotes show
T1272 3418-3422 IN denotes that
T1273 3949-3950 NN denotes B
T1274 3423-3428 JJ denotes Runx3
T1275 3429-3432 CC denotes and
T1276 3433-3438 JJ denotes T-box
T1277 3439-3446 NNS denotes factors
T1278 3447-3462 RB denotes synergistically
T1279 3463-3471 VB denotes regulate
T1280 3472-3475 NNP denotes CTL
T1281 3476-3491 NN denotes differentiation
T1282 3492-3495 CC denotes and
T1283 3496-3504 NN denotes function
T1284 3504-3505 . denotes .
T1285 3506-3511 NN denotes T-bet
T1286 3512-3514 VBZ denotes is
T1287 3515-3522 JJ denotes induced
T1288 3523-3530 RB denotes quickly
T1289 3531-3535 IN denotes upon
T1290 3536-3539 NNP denotes TCR
T1291 3540-3551 NN denotes stimulation
T1292 3552-3555 CC denotes and
T1293 3556-3558 VBZ denotes is
T1294 3559-3567 VBN denotes required
T1295 3568-3571 IN denotes for
T1296 3572-3577 JJ denotes early
T1297 3578-3589 NN denotes programming
T1298 3590-3592 IN denotes of
T1299 3593-3601 NN denotes cytokine
T1300 3602-3612 NN denotes production
T1301 3613-3614 -LRB- denotes (
T1302 3614-3616 CD denotes 17
T1303 3616-3617 -RRB- denotes )
T1304 3617-3618 , denotes ,
T1305 3619-3626 IN denotes whereas
T1306 3627-3632 NNP denotes Eomes
T1307 3633-3635 VBZ denotes is
T1308 3636-3643 JJ denotes induced
T1309 3644-3649 RB denotes later
T1310 3650-3656 IN denotes during
T1311 3657-3672 NN denotes differentiation
T1312 3673-3676 CC denotes and
T1313 3677-3685 VBZ denotes sustains
T1314 3686-3691 JJ denotes IFN-γ
T1315 3692-3702 NN denotes expression
T1316 3702-3703 . denotes .
T1317 3704-3709 NN denotes Runx3
T1318 3710-3712 VBZ denotes is
T1319 3713-3721 VBN denotes required
T1320 3722-3725 IN denotes for
T1321 3726-3731 NNP denotes Eomes
T1322 3732-3735 CC denotes and
T1323 3736-3744 JJ denotes perforin
T1324 3745-3755 NN denotes expression
T1325 3755-3756 , denotes ,
T1326 3757-3760 CC denotes and
T1327 3761-3765 DT denotes both
T1328 3766-3771 NNPS denotes Eomes
T1329 3772-3775 CC denotes and
T1330 3776-3781 NNP denotes Runx3
T1331 3782-3786 NN denotes bind
T1332 3787-3789 IN denotes at
T1333 3790-3804 NN denotes the Prf1 locus
T1334 3804-3805 : denotes ;
T1335 3806-3808 IN denotes in
T1336 3809-3817 NN denotes contrast
T1337 3817-3818 , denotes ,
T1338 3819-3827 NN denotes perforin
T1339 3828-3838 NN denotes expression
T1340 3839-3841 VBZ denotes is
T1341 3842-3852 JJ denotes unaffected
T1342 3853-3855 IN denotes in
T1343 3856-3861 NNP denotes T-bet
T1344 3862-3871 JJ denotes deficient
T1345 3872-3877 NNS denotes cells
T1346 3877-3878 . denotes .
T1347 3879-3880 NNP denotes T
T1348 3881-3886 NNS denotes cells
T1349 3887-3894 VBG denotes lacking
T1350 3895-3900 JJ denotes Runx3
T1351 3901-3905 NN denotes show
T1352 3906-3915 VBD denotes decreased
T1353 3916-3926 NN denotes expression
T1354 3927-3929 IN denotes of
T1355 3930-3935 NNP denotes IFN-γ
T1356 3936-3939 CC denotes and
T1357 3940-3948 NN denotes granzyme
T1358 3950-3951 , denotes ,
T1359 3952-3955 CC denotes and
T1360 3956-3961 NNP denotes Runx3
T1361 3962-3966 RB denotes also
T1362 3967-3972 VBZ denotes binds
T1363 3973-3976 DT denotes the
T1364 3977-3985 NN denotes promoter
T1365 3986-3993 NNS denotes regions
T1366 3994-3996 IN denotes of
T1367 3997-4020 NNS denotes the Ifng and Gzmb genes
T1368 4020-4021 . denotes .
T1369 4022-4034 RB denotes Collectively
T1370 4034-4035 , denotes ,
T1371 4036-4041 DT denotes these
T1372 4042-4049 NNS denotes results
T1373 4050-4057 VBP denotes provide
T1374 4058-4066 NN denotes evidence
T1375 4067-4070 IN denotes for
T1376 4071-4072 DT denotes a
T1377 4073-4080 JJ denotes complex
T1378 4081-4096 JJ denotes transcriptional
T1379 4097-4104 NN denotes network
T1380 4105-4107 IN denotes in
T1381 4108-4113 WDT denotes which
T1382 4114-4119 NNP denotes Runx3
T1383 4120-4122 VBZ denotes is
T1384 4123-4124 DT denotes a
T1385 4125-4132 JJ denotes primary
T1386 4133-4142 NN denotes regulator
T1387 4143-4161 NNP denotes of Gzmb expression
T1388 4162-4165 CC denotes but
T1389 4166-4176 NNS denotes synergizes
T1390 4177-4181 IN denotes with
T1391 4182-4187 NNP denotes T-bet
T1392 4188-4191 CC denotes and
T1393 4192-4197 NNP denotes Eomes
T1394 4197-4198 , denotes ,
T1395 4199-4211 RB denotes respectively
T1396 4211-4212 , denotes ,
T1397 4213-4215 TO denotes to
T1398 4216-4223 VB denotes promote
T1399 4224-4237 NN denotes transcription
T1400 4238-4240 IN denotes of
T1401 4241-4264 NNS denotes the Ifng and Prf1 genes
T1402 4264-4265 . denotes .
T2702 4291-4292 DT denotes A
T2703 4293-4297 NN denotes cell
T2704 4298-4305 NN denotes culture
T2705 4306-4312 NN denotes system
T2706 4313-4315 TO denotes to
T2707 4316-4323 VB denotes monitor
T2708 4324-4332 NN denotes effector
T2709 4333-4336 NNP denotes CTL
T2710 4337-4352 NN denotes differentiation
T2711 4353-4355 PRP denotes We
T2712 4356-4360 VBD denotes used
T2713 4361-4362 DT denotes a
T2714 4363-4369 JJ denotes simple
T2715 4370-4374 NN denotes cell
T2716 4375-4382 NN denotes culture
T2717 4383-4389 NN denotes system
T2718 4390-4392 TO denotes to
T2719 4393-4400 VB denotes examine
T2720 4401-4404 DT denotes the
T2721 4405-4413 NNS denotes kinetics
T2722 4414-4416 IN denotes of
T2723 4417-4425 NN denotes effector
T2724 4426-4430 NN denotes gene
T2725 4431-4441 NN denotes expression
T2726 4442-4448 IN denotes during
T2727 4449-4452 NNP denotes CD8
T2728 4452-4453 NNP denotes +
T2729 4454-4455 NNP denotes T
T2730 4456-4460 NN denotes cell
T2731 4461-4476 NN denotes differentiation
T2732 4476-4477 . denotes .
T2733 4478-4483 JJ denotes Naive
T2734 4484-4487 NNP denotes CD8
T2735 4487-4488 CD denotes +
T2736 4489-4490 NNP denotes T
T2737 4491-4496 NNS denotes cells
T2738 4497-4501 IN denotes from
T2739 4502-4505 NNP denotes P14
T2740 4506-4509 NNP denotes TCR
T2741 4510-4520 JJ denotes transgenic
T2742 4521-4525 NNS denotes mice
T2743 4526-4530 VBD denotes were
T2744 4531-4540 VBN denotes activated
T2745 4541-4544 IN denotes for
T2746 4545-4546 CD denotes 2
T2747 4547-4548 LS denotes d
T2748 4549-4553 IN denotes with
T2749 4554-4562 JJ denotes anti-CD3
T2750 4563-4566 CC denotes and
T2751 4567-4576 JJ denotes anti-CD28
T2752 4577-4579 CC denotes or
T2753 4580-4584 IN denotes with
T2754 4585-4592 JJ denotes splenic
T2755 4593-4597 NNS denotes APCs
T2756 4598-4600 IN denotes in
T2757 4601-4604 DT denotes the
T2758 4605-4613 NN denotes presence
T2759 4614-4616 IN denotes of
T2760 4617-4621 JJ denotes Gp33
T2761 4622-4629 NN denotes peptide
T2762 4629-4630 , denotes ,
T2763 4631-4634 CC denotes and
T2764 4635-4639 VBD denotes were
T2765 4640-4648 VBN denotes cultured
T2766 4649-4651 IN denotes in
T2767 4652-4657 NNS denotes media
T2768 4658-4668 VBG denotes containing
T2769 4669-4672 CD denotes 100
T2770 4673-4677 NNP denotes U/ml
T2771 4678-4680 IN denotes of
T2772 4681-4692 JJ denotes recombinant
T2773 4693-4698 JJ denotes human
T2774 4699-4703 NN denotes IL-2
T2775 4704-4705 -LRB- denotes (
T2776 4705-4711 NN denotes rhIL-2
T2777 4711-4712 -RRB- denotes )
T2778 4712-4713 . denotes .
T2779 4714-4716 PRP denotes We
T2780 4717-4721 VBD denotes used
T2781 4722-4725 NNP denotes TCR
T2782 4726-4736 JJ denotes transgenic
T2783 4737-4741 NNS denotes mice
T2784 4742-4745 IN denotes for
T2785 4746-4751 DT denotes these
T2786 4752-4763 NNS denotes experiments
T2787 4764-4771 IN denotes because
T2788 4772-4776 PRP denotes they
T2789 4777-4784 VBP denotes provide
T2790 4785-4786 DT denotes a
T2791 4787-4795 JJ denotes reliable
T2792 4796-4802 NN denotes source
T2793 4803-4805 IN denotes of
T2794 4806-4809 CD denotes CD8
T2795 4809-4810 CD denotes +
T2796 4811-4812 NNP denotes T
T2797 4813-4818 NNS denotes cells
T2798 4819-4823 WDT denotes that
T2799 4824-4827 VBP denotes are
T2800 4828-4833 RB denotes truly
T2801 4834-4839 JJ denotes naive
T2802 4839-4840 : denotes ;
T2803 4841-4848 RB denotes however
T2804 4848-4849 , denotes ,
T2805 4850-4852 PRP denotes we
T2806 4853-4858 VBD denotes chose
T2807 4859-4862 RB denotes not
T2808 4863-4865 TO denotes to
T2809 4866-4875 VB denotes stimulate
T2810 4876-4881 NNS denotes cells
T2811 4882-4886 IN denotes with
T2812 4887-4894 NN denotes antigen
T2813 4895-4897 IN denotes in
T2814 4898-4902 JJS denotes most
T2815 4903-4914 NNS denotes experiments
T2816 4915-4917 RB denotes so
T2817 4918-4920 IN denotes as
T2818 4921-4923 TO denotes to
T2819 4924-4929 VB denotes avoid
T2820 4930-4943 NN denotes contamination
T2821 4944-4948 IN denotes with
T2822 4949-4957 NNS denotes proteins
T2823 4958-4961 CC denotes and
T2824 4962-4969 JJ denotes nucleic
T2825 4970-4975 NNS denotes acids
T2826 4976-4983 VBN denotes derived
T2827 4984-4988 IN denotes from
T2828 4989-4993 NNS denotes APCs
T2829 4993-4994 . denotes .
T2830 4995-5000 EX denotes There
T2831 5001-5005 VBD denotes were
T2832 5006-5010 RB denotes only
T2833 5011-5016 JJ denotes minor
T2834 5017-5028 NNS denotes differences
T2835 5029-5031 IN denotes in
T2836 5032-5036 NN denotes gene
T2837 5037-5047 NN denotes expression
T2838 5048-5054 IN denotes during
T2839 5055-5070 NN denotes differentiation
T2840 5071-5078 VBN denotes induced
T2841 5079-5081 IN denotes by
T2842 5082-5093 NNP denotes antigen/APC
T2843 5094-5100 CC denotes versus
T2844 5101-5109 JJ denotes anti-CD3
T2845 5109-5110 NN denotes /
T2846 5110-5119 NN denotes anti-CD28
T2847 5119-5120 , denotes ,
T2848 5121-5124 CC denotes and
T2849 5125-5128 DT denotes the
T2850 5129-5134 JJ denotes major
T2851 5135-5146 NNS denotes conclusions
T2852 5147-5156 VBN denotes presented
T2853 5157-5159 IN denotes in
T2854 5160-5164 DT denotes this
T2855 5165-5171 NN denotes report
T2856 5172-5175 VBP denotes are
T2857 5176-5179 DT denotes the
T2858 5180-5184 JJ denotes same
T2859 5185-5188 IN denotes for
T2860 5189-5193 DT denotes both
T2861 5194-5204 VBG denotes activating
T2862 5205-5215 NNS denotes conditions
T2863 5215-5216 . denotes .
T2864 5217-5222 IN denotes Under
T2865 5223-5226 PRP$ denotes our
T2866 5227-5234 NN denotes culture
T2867 5235-5245 NNS denotes conditions
T2868 5245-5246 , denotes ,
T2869 5247-5256 VBN denotes activated
T2870 5257-5260 NNP denotes CD8
T2871 5260-5261 CD denotes +
T2872 5262-5263 NNP denotes T
T2873 5264-5269 NNS denotes cells
T2874 5270-5278 VBD denotes expanded
T2875 5279-5292 RB denotes exponentially
T2876 5293-5296 CC denotes and
T2877 5297-5308 VBN denotes accumulated
T2878 5309-5312 IN denotes for
T2879 5313-5314 NN denotes >
T2880 5314-5315 CD denotes 8
T2881 5316-5317 LS denotes d
T2882 5317-5318 . denotes .
T2883 5319-5321 PRP denotes We
T2884 5322-5329 VBD denotes limited
T2885 5330-5333 PRP$ denotes our
T2886 5334-5342 NN denotes analysis
T2887 5343-5345 TO denotes to
T2888 5346-5349 DT denotes the
T2889 5350-5355 JJ denotes first
T2890 5356-5357 CD denotes 6
T2891 5358-5359 CD denotes 8
T2892 5360-5361 LS denotes d
T2893 5362-5367 IN denotes after
T2894 5368-5378 NN denotes activation
T2895 5378-5379 , denotes ,
T2896 5380-5381 DT denotes a
T2897 5382-5388 NN denotes period
T2898 5389-5393 WDT denotes that
T2899 5394-5403 VBZ denotes coincides
T2900 5404-5408 IN denotes with
T2901 5409-5415 JJ denotes clonal
T2902 5416-5425 NN denotes expansion
T2903 5426-5428 IN denotes of
T2904 5429-5432 CD denotes CD8
T2905 5432-5433 CD denotes +
T2906 5434-5435 NNP denotes T
T2907 5436-5441 NNS denotes cells
T2908 5442-5447 IN denotes after
T2909 5448-5458 NN denotes activation
T2910 5459-5461 IN denotes in
T2911 5462-5466 NN denotes vivo
T2912 5466-5467 . denotes .
T3380 5469-5477 JJ denotes Distinct
T3381 5478-5488 NN denotes expression
T3382 5489-5497 NNS denotes kinetics
T3383 5498-5500 IN denotes of
T3384 5501-5509 NN denotes perforin
T3385 5510-5513 CC denotes and
T3386 5514-5522 NN denotes granzyme
T3387 5523-5524 NN denotes B
T3388 5525-5531 IN denotes during
T3389 5532-5535 NNP denotes CTL
T3390 5536-5547 NN denotes development
T3391 5548-5550 IN denotes in
T3392 5551-5558 NN denotes culture
T3393 5559-5562 PRP$ denotes Our
T3394 5563-5574 NNS denotes experiments
T3395 5575-5583 VBD denotes revealed
T3396 5584-5589 JJ denotes clear
T3397 5590-5601 NNS denotes differences
T3398 5602-5604 IN denotes in
T3399 5605-5608 DT denotes the
T3400 5609-5617 NNS denotes kinetics
T3401 5618-5620 IN denotes of
T3402 5621-5629 NN denotes perforin
T3403 5629-5630 , denotes ,
T3404 5631-5639 NN denotes granzyme
T3405 5640-5641 NNP denotes B
T3406 5641-5642 , denotes ,
T3407 5643-5646 CC denotes and
T3408 5647-5655 NN denotes cytokine
T3409 5656-5666 NN denotes expression
T3410 5667-5673 IN denotes during
T3411 5674-5677 NNP denotes CD8
T3412 5677-5678 NNP denotes +
T3413 5679-5680 NNP denotes T
T3414 5681-5685 NN denotes cell
T3415 5686-5696 NN denotes activation
T3416 5697-5698 -LRB- denotes (
T3417 5698-5702 FW denotes Fig.
T3418 5703-5704 LS denotes 1
T3419 5704-5705 -RRB- denotes )
T3420 5705-5706 . denotes .
T3421 5707-5712 JJ denotes Naive
T3422 5713-5714 NN denotes T
T3423 5715-5720 NNS denotes cells
T3424 5721-5727 VBD denotes showed
T3425 5728-5738 JJ denotes detectable
T3426 5739-5749 NN denotes expression
T3427 5750-5752 IN denotes of
T3428 5753-5761 NN denotes perforin
T3429 5762-5766 NNP denotes mRNA
T3430 5767-5769 RB denotes as
T3431 5770-5774 RB denotes well
T3432 5775-5777 IN denotes as
T3433 5778-5786 NN denotes perforin
T3434 5787-5794 NN denotes protein
T3435 5795-5796 -LRB- denotes (
T3436 5796-5800 FW denotes Fig.
T3437 5801-5802 CD denotes 1
T3438 5802-5803 , denotes ,
T3439 5804-5805 DT denotes A
T3440 5806-5807 NNP denotes D
T3441 5807-5808 -RRB- denotes )
T3442 5808-5809 . denotes .
T3443 5810-5818 JJ denotes Relative
T3444 5819-5821 TO denotes to
T3445 5822-5825 PRP$ denotes its
T3446 5826-5836 NN denotes expression
T3447 5837-5839 IN denotes in
T3448 5840-5845 JJ denotes naive
T3449 5846-5847 NNP denotes T
T3450 5848-5853 NNS denotes cells
T3451 5853-5854 , denotes ,
T3452 5855-5863 NN denotes perforin
T3453 5864-5865 -LRB- denotes (
T3454 5865-5869 NNP denotes Prf1
T3455 5869-5870 -RRB- denotes )
T3456 5871-5875 NNP denotes mRNA
T3457 5876-5886 NN denotes expression
T3458 5887-5890 VBD denotes did
T3459 5891-5894 RB denotes not
T3460 5895-5903 VB denotes increase
T3461 5904-5915 RB denotes appreciably
T3462 5916-5918 IN denotes at
T3463 5919-5922 NN denotes day
T3464 5923-5924 CD denotes 2
T3465 5925-5928 CC denotes but
T3466 5929-5935 VBD denotes showed
T3467 5936-5937 DT denotes a
T3468 5938-5950 JJ denotes reproducible
T3469 5951-5959 NN denotes decrease
T3470 5960-5962 IN denotes at
T3471 5963-5966 NN denotes day
T3472 5967-5968 CD denotes 4
T3473 5968-5969 , denotes ,
T3474 5970-5978 VBN denotes followed
T3475 5979-5981 IN denotes by
T3476 5982-5988 JJ denotes robust
T3477 5989-6001 NN denotes reexpression
T3478 6002-6009 IN denotes between
T3479 6010-6014 NNS denotes days
T3480 6015-6016 CD denotes 4
T3481 6017-6020 CC denotes and
T3482 6021-6022 CD denotes 8
T3483 6023-6024 -LRB- denotes (
T3484 6024-6028 NN denotes Fig.
T3485 6029-6030 CD denotes 1
T3486 6030-6031 , denotes ,
T3487 6032-6033 DT denotes A
T3488 6034-6035 NNP denotes D
T3489 6035-6036 -RRB- denotes )
T3490 6036-6037 . denotes .
T3491 6038-6040 IN denotes In
T3492 6041-6049 NN denotes contrast
T3493 6049-6050 , denotes ,
T3494 6051-6059 NN denotes granzyme
T3495 6060-6061 NNP denotes B
T3496 6062-6063 -LRB- denotes (
T3497 6063-6067 NNP denotes Gzmb
T3498 6067-6068 -RRB- denotes )
T3499 6069-6073 NNP denotes mRNA
T3500 6074-6077 VBD denotes was
T3501 6078-6081 JJ denotes low
T3502 6082-6084 CC denotes or
T3503 6085-6097 JJ denotes undetectable
T3504 6098-6100 IN denotes in
T3505 6101-6106 JJ denotes naive
T3506 6107-6108 NNP denotes T
T3507 6109-6114 NNS denotes cells
T3508 6115-6118 CC denotes but
T3509 6119-6122 VBD denotes was
T3510 6123-6131 RB denotes strongly
T3511 6132-6144 JJ denotes up-regulated
T3512 6145-6147 IN denotes by
T3513 6148-6151 NN denotes day
T3514 6152-6153 CD denotes 2
T3515 6154-6159 IN denotes after
T3516 6160-6171 NN denotes stimulation
T3517 6172-6175 CC denotes and
T3518 6176-6185 VBD denotes increased
T3519 6186-6199 RB denotes progressively
T3520 6200-6205 IN denotes until
T3521 6206-6209 NN denotes day
T3522 6210-6211 CD denotes 6
T3523 6212-6213 -LRB- denotes (
T3524 6213-6217 NN denotes Fig.
T3525 6218-6219 CD denotes 1
T3526 6219-6220 , denotes ,
T3527 6221-6222 DT denotes A
T3528 6223-6226 CC denotes and
T3529 6227-6228 NNP denotes B
T3530 6228-6229 -RRB- denotes )
T3531 6229-6230 : denotes ;
T3532 6231-6240 RB denotes similarly
T3533 6240-6241 , denotes ,
T3534 6242-6250 NN denotes granzyme
T3535 6251-6252 NNP denotes B
T3536 6253-6260 NN denotes protein
T3537 6261-6264 VBD denotes was
T3538 6265-6274 VBN denotes expressed
T3539 6275-6277 IN denotes by
T3540 6278-6281 NN denotes day
T3541 6282-6283 CD denotes 4
T3542 6284-6287 CC denotes and
T3543 6288-6296 VBD denotes remained
T3544 6297-6301 JJ denotes high
T3545 6302-6307 IN denotes until
T3546 6308-6311 NN denotes day
T3547 6312-6313 CD denotes 6
T3548 6314-6315 -LRB- denotes (
T3549 6315-6319 NN denotes Fig.
T3550 6320-6321 CD denotes 1
T3551 6322-6323 NNP denotes E
T3552 6323-6324 -RRB- denotes )
T3553 6324-6325 . denotes .
T3554 6326-6328 IN denotes As
T3555 6329-6337 VBN denotes expected
T3556 6337-6338 , denotes ,
T3557 6339-6340 DT denotes a
T3558 6341-6346 JJ denotes small
T3559 6347-6355 NN denotes fraction
T3560 6356-6358 IN denotes of
T3561 6359-6364 JJ denotes naive
T3562 6365-6366 NNP denotes T
T3563 6367-6372 NNS denotes cells
T3564 6373-6382 VBD denotes expressed
T3565 6383-6386 DT denotes the
T3566 6387-6396 NNS denotes cytokines
T3567 6397-6402 NNP denotes IFN-γ
T3568 6403-6406 CC denotes and
T3569 6407-6410 NNP denotes TNF
T3570 6411-6413 IN denotes in
T3571 6414-6422 NN denotes response
T3572 6423-6425 TO denotes to
T3573 6426-6437 NN denotes stimulation
T3574 6437-6438 , denotes ,
T3575 6439-6442 CC denotes and
T3576 6443-6447 DT denotes this
T3577 6448-6456 NN denotes capacity
T3578 6457-6466 VBD denotes increased
T3579 6467-6480 RB denotes significantly
T3580 6481-6483 IN denotes in
T3581 6484-6498 JJ denotes differentiated
T3582 6499-6504 NNS denotes cells
T3583 6505-6506 -LRB- denotes (
T3584 6506-6510 NNP denotes Fig.
T3585 6511-6512 CD denotes 1
T3586 6513-6514 NNP denotes E
T3587 6514-6515 : denotes ;
T3588 6516-6519 VB denotes see
T3589 6520-6524 RB denotes also
T3590 6525-6529 JJ denotes Fig.
T3591 6530-6531 CD denotes 2
T3592 6532-6533 NN denotes A
T3593 6533-6534 -RRB- denotes )
T3594 6534-6535 . denotes .
T3595 7844-7846 PRP denotes We
T3596 7847-7856 VBD denotes evaluated
T3597 7857-7874 JJ denotes antigen-dependent
T3598 7875-7884 JJ denotes cytolytic
T3599 7885-7893 NN denotes function
T3600 7894-7896 IN denotes in
T3601 7897-7898 DT denotes a
T3602 7899-7909 JJ denotes short-term
T3603 7910-7915 NN denotes assay
T3604 7916-7918 IN denotes in
T3605 7919-7924 WDT denotes which
T3606 7925-7931 NN denotes target
T3607 7932-7936 NN denotes cell
T3608 7937-7942 NN denotes death
T3609 7943-7946 VBD denotes was
T3610 7947-7955 VBN denotes measured
T3611 7956-7962 IN denotes within
T3612 7963-7964 CD denotes 2
T3613 7965-7966 NN denotes h
T3614 7967-7968 -LRB- denotes (
T3615 7968-7972 FW denotes Fig.
T3616 7973-7974 CD denotes 1
T3617 7975-7976 NN denotes F
T3618 7976-7977 -RRB- denotes )
T3619 7977-7978 . denotes .
T3620 7979-7981 IN denotes By
T3621 7982-7990 VBG denotes limiting
T3622 7991-7994 DT denotes the
T3623 7995-8003 NN denotes duration
T3624 8004-8006 IN denotes of
T3625 8007-8010 NNP denotes TCR
T3626 8011-8022 NN denotes stimulation
T3627 8022-8023 , denotes ,
T3628 8024-8028 DT denotes this
T3629 8029-8037 NN denotes strategy
T3630 8038-8047 VBZ denotes minimizes
T3631 8048-8057 NN denotes cytolysis
T3632 8058-8067 JJ denotes secondary
T3633 8068-8070 TO denotes to
T3634 8071-8074 JJ denotes new
T3635 8075-8079 NN denotes gene
T3636 8080-8090 NN denotes expression
T3637 8091-8097 IN denotes during
T3638 8098-8101 DT denotes the
T3639 8102-8108 NN denotes period
T3640 8109-8111 IN denotes of
T3641 8112-8115 DT denotes the
T3642 8116-8121 NN denotes assay
T3643 8121-8122 . denotes .
T3644 8123-8128 JJ denotes Naive
T3645 8129-8130 NN denotes T
T3646 8131-8136 NNS denotes cells
T3647 8137-8140 VBD denotes did
T3648 8141-8144 RB denotes not
T3649 8145-8152 NN denotes display
T3650 8153-8164 JJ denotes significant
T3651 8165-8174 JJ denotes cytolytic
T3652 8175-8183 NN denotes function
T3653 8184-8186 IN denotes in
T3654 8187-8191 DT denotes this
T3655 8192-8202 JJ denotes short-term
T3656 8203-8208 NN denotes assay
T3657 8209-8210 -LRB- denotes (
T3658 8210-8221 JJ denotes unpublished
T3659 8222-8226 NNS denotes data
T3660 8226-8227 -RRB- denotes )
T3661 8227-8228 , denotes ,
T3662 8229-8233 RBS denotes most
T3663 8234-8240 JJ denotes likely
T3664 8241-8248 IN denotes because
T3665 8249-8253 PRP denotes they
T3666 8254-8261 VBP denotes express
T3667 8262-8270 NN denotes immature
T3668 8271-8272 -LRB- denotes (
T3669 8272-8283 JJ denotes unprocessed
T3670 8283-8284 -RRB- denotes )
T3671 8285-8290 NNS denotes forms
T3672 8291-8293 IN denotes of
T3673 8294-8302 NN denotes perforin
T3674 8303-8306 CC denotes and
T3675 8307-8311 VBP denotes lack
T3676 8312-8315 DT denotes the
T3677 8316-8324 NN denotes capacity
T3678 8325-8327 TO denotes to
T3679 8328-8339 VB denotes degranulate
T3680 8340-8341 -LRB- denotes (
T3681 8341-8343 CD denotes 18
T3682 8343-8344 , denotes ,
T3683 8345-8347 CD denotes 19
T3684 8347-8348 -RRB- denotes )
T3685 8348-8349 . denotes .
T3686 8350-8354 RB denotes Even
T3687 8355-8360 IN denotes after
T3688 8361-8371 NN denotes activation
T3689 8372-8375 IN denotes for
T3690 8376-8377 CD denotes 2
T3691 8378-8380 CC denotes or
T3692 8381-8382 CD denotes 4
T3693 8383-8384 LS denotes d
T3694 8384-8385 , denotes ,
T3695 8386-8389 DT denotes the
T3696 8390-8395 NNS denotes cells
T3697 8396-8402 VBD denotes showed
T3698 8403-8407 JJ denotes poor
T3699 8408-8417 JJ denotes cytolytic
T3700 8418-8426 NN denotes activity
T3701 8427-8428 -LRB- denotes (
T3702 8428-8432 FW denotes Fig.
T3703 8433-8434 CD denotes 1
T3704 8435-8436 NN denotes F
T3705 8436-8437 -RRB- denotes )
T3706 8437-8438 , denotes ,
T3707 8439-8441 IN denotes in
T3708 8442-8450 JJ denotes striking
T3709 8451-8459 NN denotes contrast
T3710 8460-8462 TO denotes to
T3711 8463-8468 PRP$ denotes their
T3712 8469-8477 NN denotes capacity
T3713 8478-8481 IN denotes for
T3714 8482-8491 JJ denotes efficient
T3715 8492-8500 NN denotes cytokine
T3716 8501-8511 NN denotes production
T3717 8512-8513 -LRB- denotes (
T3718 8513-8517 FW denotes Fig.
T3719 8518-8519 CD denotes 1
T3720 8520-8521 NNP denotes E
T3721 8521-8522 -RRB- denotes )
T3722 8522-8523 . denotes .
T3723 8524-8528 RB denotes Only
T3724 8529-8534 NNS denotes cells
T3725 8535-8543 VBD denotes cultured
T3726 8544-8549 IN denotes until
T3727 8550-8553 NN denotes day
T3728 8554-8555 CD denotes 6
T3729 8556-8565 VBD denotes displayed
T3730 8566-8572 JJ denotes robust
T3731 8573-8585 NN denotes cytotoxicity
T3732 8585-8586 , denotes ,
T3733 8587-8589 IN denotes as
T3734 8590-8596 VBN denotes judged
T3735 8597-8599 IN denotes by
T3736 8600-8605 PRP$ denotes their
T3737 8606-8613 NN denotes ability
T3738 8614-8616 TO denotes to
T3739 8617-8623 VB denotes induce
T3740 8624-8633 NN denotes apoptosis
T3741 8634-8636 IN denotes in
T3742 8637-8638 DT denotes a
T3743 8639-8644 JJ denotes large
T3744 8645-8651 NN denotes number
T3745 8652-8654 IN denotes of
T3746 8655-8661 NN denotes target
T3747 8662-8667 NNS denotes cells
T3748 8668-8669 -LRB- denotes (
T3749 8669-8673 NNP denotes Fig.
T3750 8674-8675 CD denotes 1
T3751 8676-8677 NN denotes F
T3752 8677-8678 -RRB- denotes )
T3753 8678-8679 . denotes .
T3754 8680-8685 DT denotes These
T3755 8686-8693 NNS denotes results
T3756 8694-8698 VBP denotes show
T3757 8699-8703 IN denotes that
T3758 8704-8709 IN denotes after
T3759 8710-8711 DT denotes a
T3760 8712-8718 JJ denotes strong
T3761 8719-8726 NN denotes priming
T3762 8727-8735 NN denotes stimulus
T3763 8736-8743 IN denotes through
T3764 8744-8748 NNS denotes TCRs
T3765 8749-8752 CC denotes and
T3766 8753-8767 JJ denotes co-stimulatory
T3767 8768-8777 NNS denotes receptors
T3768 8778-8780 IN denotes in
T3769 8781-8786 NN denotes vitro
T3770 8786-8787 , denotes ,
T3771 8788-8796 NN denotes granzyme
T3772 8797-8798 NNP denotes B
T3773 8799-8809 NN denotes expression
T3774 8810-8813 CC denotes and
T3775 8814-8817 DT denotes the
T3776 8818-8825 NN denotes ability
T3777 8826-8828 TO denotes to
T3778 8829-8836 VB denotes produce
T3779 8837-8845 NN denotes effector
T3780 8846-8855 NNS denotes cytokines
T3781 8856-8859 VBP denotes are
T3782 8860-8870 VBN denotes programmed
T3783 8871-8876 RB denotes early
T3784 8876-8877 , denotes ,
T3785 8878-8885 IN denotes whereas
T3786 8886-8894 NN denotes perforin
T3787 8895-8905 NN denotes expression
T3788 8906-8909 CC denotes and
T3789 8910-8919 JJ denotes cytolytic
T3790 8920-8928 NN denotes function
T3791 8929-8932 VBP denotes are
T3792 8933-8940 JJ denotes induced
T3793 8941-8946 RB denotes later
T3794 8946-8947 , denotes ,
T3795 8948-8954 IN denotes during
T3796 8955-8958 DT denotes the
T3797 8959-8964 NN denotes phase
T3798 8965-8967 IN denotes of
T3799 8968-8974 JJ denotes clonal
T3800 8975-8984 NN denotes expansion
T3801 8985-8987 IN denotes in
T3802 8988-8992 NN denotes IL-2
T3803 8992-8993 . denotes .
T3804 8994-9003 RB denotes Therefore
T3805 9003-9004 , denotes ,
T3806 9005-9008 DT denotes the
T3807 9009-9012 CD denotes two
T3808 9013-9018 JJ denotes major
T3809 9019-9027 NN denotes effector
T3810 9028-9037 NNS denotes functions
T3811 9038-9040 IN denotes of
T3812 9041-9044 NNP denotes CTL
T3813 9044-9045 , denotes ,
T3814 9046-9054 NN denotes cytokine
T3815 9055-9065 NN denotes production
T3816 9066-9069 CC denotes and
T3817 9070-9079 JJ denotes cytolytic
T3818 9080-9088 NN denotes activity
T3819 9088-9089 , denotes ,
T3820 9090-9093 VBP denotes are
T3821 9094-9097 RB denotes not
T3822 9098-9111 RB denotes intrinsically
T3823 9112-9123 VBN denotes coregulated
T3824 9123-9124 . denotes .
T4777 9126-9134 JJ denotes Distinct
T4778 9135-9143 NNS denotes kinetics
T4779 9144-9146 IN denotes of
T4780 9147-9152 NNP denotes T-bet
T4781 9153-9156 CC denotes and
T4782 9157-9162 NNP denotes Eomes
T4783 9163-9173 NN denotes expression
T4784 9174-9180 IN denotes during
T4785 9181-9184 NNP denotes CTL
T4786 9185-9200 NN denotes differentiation
T4787 9201-9204 DT denotes The
T4788 9205-9210 NN denotes T-box
T4789 9211-9224 NN denotes transcription
T4790 9225-9232 NNS denotes factors
T4791 9233-9238 NNP denotes T-bet
T4792 9239-9242 CC denotes and
T4793 9243-9248 NNP denotes Eomes
T4794 9249-9253 VBP denotes have
T4795 9254-9258 VBN denotes been
T4796 9259-9265 VBN denotes linked
T4797 9266-9268 TO denotes to
T4798 9269-9272 DT denotes the
T4799 9273-9283 NN denotes regulation
T4800 9284-9286 IN denotes of
T4801 9287-9292 NNS denotes genes
T4802 9293-9301 VBG denotes encoding
T4803 9302-9310 NN denotes effector
T4804 9311-9320 NNS denotes cytokines
T4805 9321-9322 -LRB- denotes (
T4806 9322-9326 FW denotes e.g.
T4807 9326-9327 , denotes ,
T4808 9328-9332 NNP denotes Ifng
T4809 9332-9333 -RRB- denotes )
T4810 9334-9337 CC denotes and
T4811 9338-9343 NNS denotes genes
T4812 9344-9353 JJ denotes important
T4813 9354-9357 IN denotes for
T4814 9358-9367 JJ denotes cytolytic
T4815 9368-9376 NN denotes function
T4816 9377-9378 -LRB- denotes (
T4817 9378-9382 FW denotes e.g.
T4818 9382-9383 , denotes ,
T4819 9384-9388 NNP denotes Prf1
T4820 9389-9392 CC denotes and
T4821 9393-9397 NNP denotes GzmB
T4822 9397-9398 -RRB- denotes )
T4823 9399-9400 -LRB- denotes (
T4824 9400-9402 CD denotes 20
T4825 9402-9403 -RRB- denotes )
T4826 9403-9404 . denotes .
T4827 9405-9407 PRP denotes We
T4828 9408-9420 VBD denotes investigated
T4829 9421-9424 DT denotes the
T4830 9425-9433 NNS denotes kinetics
T4831 9434-9436 IN denotes of
T4832 9437-9447 NN denotes expression
T4833 9448-9450 IN denotes of
T4834 9451-9456 DT denotes these
T4835 9457-9470 NN denotes transcription
T4836 9471-9478 NNS denotes factors
T4837 9479-9481 IN denotes in
T4838 9482-9485 PRP$ denotes our
T4839 9486-9488 IN denotes in
T4840 9489-9494 NN denotes vitro
T4841 9495-9503 NNS denotes cultures
T4842 9504-9505 -LRB- denotes (
T4843 9505-9509 NNP denotes Fig.
T4844 9510-9511 CD denotes 1
T4845 9511-9512 , denotes ,
T4846 9513-9514 DT denotes A
T4847 9515-9516 NNP denotes D
T4848 9516-9517 -RRB- denotes )
T4849 9517-9518 . denotes .
T4850 9519-9524 NNP denotes T-bet
T4851 9525-9529 NNP denotes mRNA
T4852 9530-9533 CC denotes and
T4853 9534-9541 NN denotes protein
T4854 9542-9546 VBD denotes were
T4855 9547-9550 RB denotes not
T4856 9551-9561 JJ denotes detectable
T4857 9562-9564 IN denotes in
T4858 9565-9570 JJ denotes naive
T4859 9571-9574 NNP denotes CD8
T4860 9574-9575 CD denotes +
T4861 9576-9577 NNP denotes T
T4862 9578-9583 NNS denotes cells
T4863 9583-9584 , denotes ,
T4864 9585-9588 CC denotes but
T4865 9589-9593 VBD denotes were
T4866 9594-9602 RB denotes strongly
T4867 9603-9610 JJ denotes induced
T4868 9611-9615 IN denotes upon
T4869 9616-9619 NNP denotes TCR
T4870 9620-9627 NN denotes priming
T4871 9628-9629 -LRB- denotes (
T4872 9629-9632 NN denotes day
T4873 9633-9634 CD denotes 2
T4874 9634-9635 -RRB- denotes )
T4875 9636-9639 CC denotes and
T4876 9640-9648 VBD denotes remained
T4877 9649-9658 VBN denotes expressed
T4878 9659-9666 IN denotes through
T4879 9667-9670 NN denotes day
T4880 9671-9672 CD denotes 6
T4881 9673-9675 IN denotes of
T4882 9676-9691 NN denotes differentiation
T4883 9692-9693 -LRB- denotes (
T4884 9693-9697 FW denotes Fig.
T4885 9698-9699 CD denotes 1
T4886 9699-9700 , denotes ,
T4887 9701-9702 DT denotes A
T4888 9703-9706 CC denotes and
T4889 9707-9708 NNP denotes C
T4890 9708-9709 : denotes ;
T4891 9710-9720 VBN denotes quantified
T4892 9721-9723 IN denotes in
T4893 9724-9728 NNP denotes Fig.
T4894 9729-9730 CD denotes 1
T4895 9730-9731 , denotes ,
T4896 9732-9733 NNP denotes B
T4897 9734-9737 CC denotes and
T4898 9738-9739 NNP denotes D
T4899 9739-9740 -RRB- denotes )
T4900 9740-9741 . denotes .
T4901 9742-9744 IN denotes In
T4902 9745-9753 NN denotes contrast
T4903 9753-9754 , denotes ,
T4904 9755-9760 NNP denotes Eomes
T4905 9761-9771 NN denotes expression
T4906 9772-9775 VBD denotes was
T4907 9776-9779 JJ denotes low
T4908 9780-9782 CC denotes or
T4909 9783-9795 JJ denotes undetectable
T4910 9796-9798 IN denotes at
T4911 9799-9803 DT denotes both
T4912 9804-9807 DT denotes the
T4913 9808-9812 NNP denotes mRNA
T4914 9813-9816 CC denotes and
T4915 9817-9824 NN denotes protein
T4916 9825-9831 NNS denotes levels
T4917 9832-9834 IN denotes in
T4918 9835-9840 JJ denotes naive
T4919 9841-9844 NNP denotes CD8
T4920 9844-9845 CD denotes +
T4921 9846-9847 NNP denotes T
T4922 9848-9853 NNS denotes cells
T4923 9853-9854 , denotes ,
T4924 9855-9858 CC denotes and
T4925 9859-9862 NNP denotes TCR
T4926 9863-9870 VBG denotes priming
T4927 9871-9873 IN denotes in
T4928 9874-9881 NN denotes culture
T4929 9882-9885 VBD denotes had
T4930 9886-9890 RB denotes only
T4931 9891-9892 DT denotes a
T4932 9893-9899 JJ denotes modest
T4933 9900-9906 NN denotes effect
T4934 9907-9909 IN denotes on
T4935 9910-9913 PRP$ denotes its
T4936 9914-9924 NN denotes expression
T4937 9925-9927 IN denotes at
T4938 9928-9931 NN denotes day
T4939 9932-9933 CD denotes 2
T4940 9934-9935 -LRB- denotes (
T4941 9935-9939 NN denotes Fig.
T4942 9940-9941 CD denotes 1
T4943 9941-9942 , denotes ,
T4944 9943-9944 DT denotes A
T4945 9945-9948 CC denotes and
T4946 9949-9950 NNP denotes C
T4947 9950-9951 -RRB- denotes )
T4948 9951-9952 . denotes .
T4949 9953-9959 JJ denotes Strong
T4950 9960-9969 NN denotes induction
T4951 9970-9972 IN denotes of
T4952 9973-9978 NNP denotes Eomes
T4953 9979-9983 NNP denotes mRNA
T4954 9984-9987 CC denotes and
T4955 9988-9995 NN denotes protein
T4956 9996-9999 VBD denotes was
T4957 10000-10004 RB denotes only
T4958 10005-10013 VBN denotes observed
T4959 10014-10016 IN denotes at
T4960 10017-10020 NN denotes day
T4961 10021-10022 CD denotes 4
T4962 10023-10026 CC denotes and
T4963 10027-10032 RB denotes later
T4964 10033-10034 -LRB- denotes (
T4965 10034-10038 NNP denotes Fig.
T4966 10039-10040 CD denotes 1
T4967 10040-10041 , denotes ,
T4968 10042-10043 DT denotes A
T4969 10044-10047 CC denotes and
T4970 10048-10049 NNP denotes C
T4971 10049-10050 -RRB- denotes )
T4972 10050-10051 . denotes .
T4973 10052-10057 NNP denotes T-bet
T4974 10058-10062 NNP denotes mRNA
T4975 10063-10073 NN denotes expression
T4976 10074-10082 RB denotes slightly
T4977 10083-10091 VBD denotes preceded
T4978 10092-10095 DT denotes the
T4979 10096-10106 NN denotes expression
T4980 10107-10109 IN denotes of
T4981 10110-10114 NNP denotes GzmB
T4982 10115-10119 NNP denotes mRNA
T4983 10119-10120 : denotes ;
T4984 10121-10130 RB denotes similarly
T4985 10130-10131 , denotes ,
T4986 10132-10137 NNP denotes Eomes
T4987 10138-10142 NNP denotes mRNA
T4988 10143-10146 CC denotes and
T4989 10147-10154 NN denotes protein
T4990 10155-10159 VBD denotes were
T4991 10160-10169 VBN denotes expressed
T4992 10170-10171 RB denotes ∼
T4993 10171-10172 CD denotes 1
T4994 10173-10174 LS denotes d
T4995 10175-10180 RB denotes ahead
T4996 10181-10183 IN denotes of
T4997 10184-10187 DT denotes the
T4998 10188-10200 NN denotes reexpression
T4999 10201-10203 IN denotes of
T5000 10204-10212 NN denotes perforin
T5001 10213-10217 NNP denotes mRNA
T5002 10218-10221 CC denotes and
T5003 10222-10229 NN denotes protein
T5004 10229-10230 , denotes ,
T5005 10231-10243 RB denotes respectively
T5006 10244-10245 -LRB- denotes (
T5007 10245-10249 NNP denotes Fig.
T5008 10250-10251 CD denotes 1
T5009 10251-10252 , denotes ,
T5010 10253-10254 NNP denotes B
T5011 10255-10258 CC denotes and
T5012 10259-10260 NNP denotes D
T5013 10260-10261 -RRB- denotes )
T5014 10261-10262 . denotes .
T5015 10263-10267 DT denotes This
T5016 10268-10276 JJ denotes detailed
T5017 10277-10284 JJ denotes kinetic
T5018 10285-10293 NN denotes analysis
T5019 10294-10303 VBD denotes suggested
T5020 10304-10308 IN denotes that
T5021 10308-10309 , denotes ,
T5022 10310-10315 IN denotes under
T5023 10316-10319 PRP$ denotes our
T5024 10320-10327 NN denotes culture
T5025 10328-10338 NNS denotes conditions
T5026 10338-10339 , denotes ,
T5027 10340-10345 NNP denotes T-bet
T5028 10346-10349 CC denotes and
T5029 10350-10355 NNP denotes Eomes
T5030 10356-10366 VBP denotes contribute
T5031 10367-10369 TO denotes to
T5032 10370-10378 JJ denotes distinct
T5033 10379-10386 NNS denotes aspects
T5034 10387-10389 IN denotes of
T5035 10390-10394 NN denotes gene
T5036 10395-10408 NN denotes transcription
T5037 10409-10415 IN denotes during
T5038 10416-10419 NNP denotes CTL
T5039 10420-10435 NN denotes differentiation
T5040 10435-10436 . denotes .
T5041 10437-10442 NN denotes T-bet
T5042 10443-10445 VBZ denotes is
T5043 10446-10454 VBN denotes required
T5044 10455-10460 RB denotes early
T5045 10461-10464 IN denotes for
T5046 10465-10470 JJ denotes IFN-γ
T5047 10471-10481 NN denotes production
T5048 10481-10482 , denotes ,
T5049 10483-10486 CC denotes and
T5050 10487-10490 PRP$ denotes our
T5051 10491-10495 NNS denotes data
T5052 10496-10505 VBD denotes suggested
T5053 10506-10510 IN denotes that
T5054 10511-10516 NNP denotes Eomes
T5055 10517-10522 MD denotes might
T5056 10523-10526 RB denotes not
T5057 10527-10535 VB denotes function
T5058 10536-10542 IN denotes during
T5059 10543-10547 DT denotes this
T5060 10548-10553 JJ denotes early
T5061 10554-10560 NN denotes period
T5062 10561-10564 CC denotes but
T5063 10565-10571 RB denotes rather
T5064 10572-10577 MD denotes might
T5065 10578-10588 VB denotes contribute
T5066 10589-10594 RB denotes later
T5067 10595-10597 TO denotes to
T5068 10598-10601 DT denotes the
T5069 10602-10609 NN denotes control
T5070 10610-10612 IN denotes of
T5071 10613-10621 NN denotes perforin
T5072 10622-10632 NN denotes expression
T5073 10632-10633 . denotes .
T5074 10634-10637 PRP$ denotes Our
T5075 10638-10642 NNS denotes data
T5076 10643-10649 VBD denotes seemed
T5077 10650-10654 RBS denotes most
T5078 10655-10665 JJ denotes consistent
T5079 10666-10670 IN denotes with
T5080 10671-10672 DT denotes a
T5081 10673-10678 NN denotes model
T5082 10679-10681 IN denotes in
T5083 10682-10687 WDT denotes which
T5084 10688-10691 NNP denotes TCR
T5085 10692-10699 VBZ denotes signals
T5086 10700-10706 VB denotes induce
T5087 10707-10712 NNP denotes T-bet
T5088 10712-10713 , denotes ,
T5089 10714-10719 WDT denotes which
T5090 10720-10722 IN denotes in
T5091 10723-10727 NN denotes turn
T5092 10728-10735 VBZ denotes induces
T5093 10736-10741 NNP denotes IFN-γ
T5094 10742-10743 -LRB- denotes (
T5095 10743-10745 CD denotes 17
T5096 10745-10746 -RRB- denotes )
T5097 10747-10750 CC denotes and
T5098 10751-10759 RB denotes possibly
T5099 10760-10768 VB denotes granzyme
T5100 10769-10770 NNP denotes B
T5101 10770-10771 : denotes ;
T5102 10772-10784 RB denotes subsequently
T5103 10784-10785 , denotes ,
T5104 10786-10791 NNP denotes Eomes
T5105 10792-10794 VBZ denotes is
T5106 10795-10802 VBN denotes induced
T5107 10803-10809 IN denotes during
T5108 10810-10813 DT denotes the
T5109 10814-10820 NN denotes period
T5110 10821-10823 IN denotes of
T5111 10824-10830 JJ denotes clonal
T5112 10831-10840 NN denotes expansion
T5113 10841-10843 IN denotes in
T5114 10844-10848 NN denotes IL-2
T5115 10849-10852 CC denotes and
T5116 10853-10862 VBZ denotes activates
T5117 10863-10871 JJ denotes perforin
T5118 10872-10882 NN denotes expression
T5119 10882-10883 . denotes .
T6120 10885-10893 NNP denotes Perforin
T6121 10894-10897 CC denotes and
T6122 10898-10906 NN denotes granzyme
T6123 10907-10908 NNP denotes B
T6124 10909-10919 NN denotes expression
T6125 10920-10923 VBP denotes are
T6126 10924-10927 RB denotes not
T6127 10928-10939 RB denotes appreciably
T6128 10940-10949 VBN denotes regulated
T6129 10950-10952 IN denotes by
T6130 10953-10958 NNP denotes T-bet
T6131 10959-10961 TO denotes To
T6132 10962-10966 VB denotes test
T6133 10967-10970 DT denotes the
T6134 10971-10976 NN denotes model
T6135 10977-10985 VBN denotes outlined
T6136 10986-10988 IN denotes in
T6137 10989-10992 DT denotes the
T6138 10993-11001 JJ denotes previous
T6139 11002-11011 NN denotes paragraph
T6140 11012-11020 RB denotes directly
T6141 11020-11021 , denotes ,
T6142 11022-11024 PRP denotes we
T6143 11025-11033 VBD denotes compared
T6144 11034-11037 DT denotes the
T6145 11038-11048 NN denotes expression
T6146 11049-11051 IN denotes of
T6147 11052-11057 NNP denotes IFN-γ
T6148 11057-11058 , denotes ,
T6149 11059-11067 NN denotes perforin
T6150 11067-11068 , denotes ,
T6151 11069-11072 CC denotes and
T6152 11073-11081 NN denotes granzyme
T6153 11082-11083 NN denotes B
T6154 11084-11086 IN denotes in
T6155 11087-11090 CD denotes CD8
T6156 11090-11091 CD denotes +
T6157 11092-11093 NNP denotes T
T6158 11094-11099 NNS denotes cells
T6159 11100-11104 IN denotes from
T6160 11105-11107 NNP denotes WT
T6161 11108-11111 CC denotes and
T6162 11112-11117 NNP denotes Tbx21
T6163 11118-11119 -LRB- denotes (
T6164 11119-11124 NNP denotes T-bet
T6165 11124-11125 -RRB- denotes )
T6166 11125-11126 : denotes -
T6167 11126-11135 NN denotes deficient
T6168 11136-11140 NNS denotes mice
T6169 11140-11141 . denotes .
T6170 11142-11144 IN denotes As
T6171 11145-11153 VBN denotes expected
T6172 11154-11155 -LRB- denotes (
T6173 11155-11157 CD denotes 17
T6174 11157-11158 , denotes ,
T6175 11159-11161 CD denotes 21
T6176 11161-11162 -RRB- denotes )
T6177 11162-11163 , denotes ,
T6178 11164-11169 JJ denotes naive
T6179 11170-11175 NNP denotes Tbx21
T6180 11175-11176 FW denotes −
T6181 11176-11177 FW denotes /
T6182 11177-11178 FW denotes −
T6183 11179-11182 FW denotes CD8
T6184 11182-11183 FW denotes +
T6185 11184-11185 FW denotes T
T6186 11186-11191 NNS denotes cells
T6187 11192-11200 VBD denotes produced
T6188 11201-11206 JJ denotes IFN-γ
T6189 11207-11213 RB denotes poorly
T6190 11214-11218 IN denotes upon
T6191 11219-11229 NN denotes activation
T6192 11230-11231 -LRB- denotes (
T6193 11231-11235 JJ denotes Fig.
T6194 11236-11237 CD denotes 2
T6195 11238-11239 NN denotes A
T6196 11239-11240 -RRB- denotes )
T6197 11240-11241 . denotes .
T6198 11242-11249 RB denotes Notably
T6199 11249-11250 , denotes ,
T6200 11251-11255 DT denotes this
T6201 11256-11267 JJ denotes deleterious
T6202 11268-11274 NN denotes effect
T6203 11275-11277 IN denotes of
T6204 11278-11283 NN denotes T-bet
T6205 11284-11294 NN denotes deficiency
T6206 11295-11298 VBD denotes was
T6207 11299-11303 RB denotes only
T6208 11304-11312 VBN denotes observed
T6209 11313-11315 IN denotes in
T6210 11316-11331 VBG denotes differentiating
T6211 11332-11335 NNP denotes CD8
T6212 11335-11336 CD denotes +
T6213 11337-11338 NNP denotes T
T6214 11339-11344 NNS denotes cells
T6215 11345-11350 IN denotes until
T6216 11351-11354 NN denotes day
T6217 11355-11356 CD denotes 4
T6218 11357-11359 IN denotes of
T6219 11360-11367 NN denotes culture
T6220 11368-11371 CC denotes but
T6221 11372-11375 VBD denotes was
T6222 11376-11382 RB denotes almost
T6223 11383-11393 RB denotes completely
T6224 11394-11403 VBN denotes mitigated
T6225 11404-11406 IN denotes by
T6226 11407-11410 NN denotes day
T6227 11411-11412 CD denotes 6
T6228 11413-11414 -LRB- denotes (
T6229 11414-11418 NN denotes Fig.
T6230 11419-11420 CD denotes 2
T6231 11421-11422 NNP denotes A
T6232 11422-11423 -RRB- denotes )
T6233 11423-11424 . denotes .
T6234 11425-11429 DT denotes This
T6235 11430-11434 RBS denotes most
T6236 11435-11441 JJ denotes likely
T6237 11442-11451 VBN denotes reflected
T6238 11452-11464 NN denotes compensation
T6239 11465-11467 IN denotes by
T6240 11468-11473 NNP denotes Eomes
T6241 11473-11474 , denotes ,
T6242 11475-11480 WDT denotes which
T6243 11481-11484 VBD denotes was
T6244 11485-11493 RB denotes strongly
T6245 11494-11501 JJ denotes induced
T6246 11502-11509 IN denotes between
T6247 11510-11514 NNS denotes days
T6248 11515-11516 CD denotes 4
T6249 11517-11520 CC denotes and
T6250 11521-11522 CD denotes 6
T6251 11523-11524 -LRB- denotes (
T6252 11524-11528 NN denotes Fig.
T6253 11529-11530 CD denotes 1
T6254 11530-11531 -RRB- denotes )
T6255 11531-11532 . denotes .
T6256 11533-11535 IN denotes In
T6257 11536-11544 NN denotes contrast
T6258 11544-11545 , denotes ,
T6259 11546-11551 NNP denotes T-bet
T6260 11552-11561 JJ denotes deficient
T6261 11562-11563 NNP denotes T
T6262 11564-11569 NNS denotes cells
T6263 11570-11578 VBD denotes cultured
T6264 11579-11582 IN denotes for
T6265 11583-11584 CD denotes 6
T6266 11585-11586 LS denotes d
T6267 11587-11593 VBD denotes showed
T6268 11594-11596 DT denotes no
T6269 11597-11603 NN denotes defect
T6270 11604-11606 IN denotes in
T6271 11607-11615 NN denotes perforin
T6272 11616-11620 NNP denotes mRNA
T6273 11621-11631 NN denotes expression
T6274 11632-11633 -LRB- denotes (
T6275 11633-11637 JJ denotes Fig.
T6276 11638-11639 CD denotes 2
T6277 11640-11641 NNP denotes B
T6278 11641-11642 , denotes ,
T6279 11643-11650 VBP denotes compare
T6280 11651-11656 NNS denotes lanes
T6281 11657-11658 CD denotes 1
T6282 11659-11662 CC denotes and
T6283 11663-11664 CD denotes 4
T6284 11664-11665 -RRB- denotes )
T6285 11665-11666 . denotes .
T6286 11667-11669 PRP denotes We
T6287 11670-11682 RB denotes consistently
T6288 11683-11691 VBD denotes observed
T6289 11692-11693 DT denotes a
T6290 11694-11700 JJ denotes modest
T6291 11701-11710 NN denotes reduction
T6292 11711-11713 IN denotes in
T6293 11714-11718 NNP denotes GzmB
T6294 11719-11723 NNP denotes mRNA
T6295 11724-11726 IN denotes in
T6296 11727-11732 NNP denotes T-bet
T6297 11733-11742 JJ denotes deficient
T6298 11743-11744 NNP denotes T
T6299 11745-11750 NNS denotes cells
T6300 11751-11752 -LRB- denotes (
T6301 11752-11756 NN denotes Fig.
T6302 11757-11758 CD denotes 2
T6303 11759-11760 NNP denotes B
T6304 11760-11761 , denotes ,
T6305 11762-11769 VBP denotes compare
T6306 11770-11775 NNS denotes lanes
T6307 11776-11777 CD denotes 1
T6308 11778-11781 CC denotes and
T6309 11782-11783 CD denotes 4
T6310 11783-11784 -RRB- denotes )
T6311 11784-11785 , denotes ,
T6312 11786-11791 WDT denotes which
T6313 11792-11795 VBD denotes did
T6314 11796-11799 RB denotes not
T6315 11800-11809 VB denotes translate
T6316 11810-11814 IN denotes into
T6317 11815-11816 DT denotes a
T6318 11817-11825 NN denotes decrease
T6319 11826-11828 IN denotes in
T6320 11829-11839 NN denotes expression
T6321 11840-11842 IN denotes of
T6322 11843-11851 NN denotes granzyme
T6323 11852-11853 NNP denotes B
T6324 11854-11861 NN denotes protein
T6325 11862-11863 -LRB- denotes (
T6326 11863-11867 JJ denotes Fig.
T6327 11868-11869 CD denotes 2
T6328 11870-11871 NNP denotes C
T6329 11871-11872 -RRB- denotes )
T6330 11872-11873 . denotes .
T6331 13130-13132 TO denotes To
T6332 13133-13140 VB denotes examine
T6333 13141-13144 DT denotes the
T6334 13145-13149 NN denotes role
T6335 13150-13152 IN denotes of
T6336 13153-13158 NNP denotes Eomes
T6337 13158-13159 , denotes ,
T6338 13160-13162 PRP denotes we
T6339 13163-13173 VBD denotes transduced
T6340 13174-13179 JJ denotes naive
T6341 13180-13183 NNP denotes CD8
T6342 13183-13184 CD denotes +
T6343 13185-13186 NNP denotes T
T6344 13187-13192 NNS denotes cells
T6345 13193-13197 IN denotes from
T6346 13198-13200 NNP denotes WT
T6347 13201-13204 CC denotes and
T6348 13205-13210 NNP denotes Tbx21
T6349 13210-13211 NNP denotes −
T6350 13211-13212 VBD denotes /
T6351 13212-13213 CD denotes −
T6352 13214-13218 NNS denotes mice
T6353 13219-13223 IN denotes with
T6354 13224-13236 NNS denotes retroviruses
T6355 13237-13247 VBG denotes containing
T6356 13248-13256 JJ denotes internal
T6357 13257-13265 JJ denotes ribosome
T6358 13266-13271 NN denotes entry
T6359 13272-13276 NN denotes site
T6360 13277-13278 -LRB- denotes (
T6361 13278-13282 NNP denotes IRES
T6362 13282-13283 -RRB- denotes )
T6363 13284-13287 NNP denotes GFP
T6364 13288-13292 WDT denotes that
T6365 13293-13297 VBD denotes were
T6366 13298-13304 CC denotes either
T6367 13305-13310 JJ denotes empty
T6368 13311-13313 CC denotes or
T6369 13314-13321 VBD denotes encoded
T6370 13322-13323 DT denotes a
T6371 13324-13332 RB denotes strongly
T6372 13333-13348 VBG denotes transactivating
T6373 13349-13356 NN denotes version
T6374 13357-13359 IN denotes of
T6375 13360-13365 NNP denotes Eomes
T6376 13366-13367 -LRB- denotes (
T6377 13367-13374 NNP denotes Eo-VP16
T6378 13374-13375 -RRB- denotes )
T6379 13376-13377 -LRB- denotes (
T6380 13377-13378 CD denotes 8
T6381 13378-13379 -RRB- denotes )
T6382 13379-13380 , denotes ,
T6383 13381-13384 CC denotes and
T6384 13385-13393 VBD denotes expanded
T6385 13394-13398 PRP denotes them
T6386 13399-13402 IN denotes for
T6387 13403-13404 CD denotes 6
T6388 13405-13406 LS denotes d
T6389 13407-13412 IN denotes under
T6390 13413-13416 PRP$ denotes our
T6391 13417-13424 NN denotes culture
T6392 13425-13435 NNS denotes conditions
T6393 13435-13436 . denotes .
T6394 13437-13444 NN denotes Eo-VP16
T6395 13444-13445 , denotes ,
T6396 13446-13449 CC denotes but
T6397 13450-13453 RB denotes not
T6398 13454-13457 DT denotes the
T6399 13458-13463 JJ denotes empty
T6400 13464-13467 NNP denotes GFP
T6401 13468-13478 NN denotes retrovirus
T6402 13478-13479 , denotes ,
T6403 13480-13489 VBD denotes increased
T6404 13490-13498 NN denotes perforin
T6405 13499-13509 NN denotes expression
T6406 13510-13512 IN denotes in
T6407 13513-13517 DT denotes both
T6408 13518-13520 NNP denotes WT
T6409 13521-13524 CC denotes and
T6410 13525-13530 NNP denotes T-bet
T6411 13531-13540 JJ denotes deficient
T6412 13541-13544 NNP denotes CD8
T6413 13544-13545 CD denotes +
T6414 13546-13547 NNP denotes T
T6415 13548-13553 NNS denotes cells
T6416 13554-13555 -LRB- denotes (
T6417 13555-13559 NN denotes Fig.
T6418 13560-13561 CD denotes 2
T6419 13562-13563 NNP denotes B
T6420 13563-13564 , denotes ,
T6421 13565-13570 NNS denotes lanes
T6422 13571-13572 CD denotes 2
T6423 13572-13573 , denotes ,
T6424 13574-13575 CD denotes 3
T6425 13575-13576 , denotes ,
T6426 13577-13578 CD denotes 5
T6427 13578-13579 , denotes ,
T6428 13580-13583 CC denotes and
T6429 13584-13585 CD denotes 6
T6430 13585-13586 -RRB- denotes )
T6431 13586-13587 . denotes .
T6432 13588-13590 IN denotes As
T6433 13591-13599 VBN denotes expected
T6434 13599-13600 , denotes ,
T6435 13601-13608 NNP denotes Eo-VP16
T6436 13609-13613 RB denotes also
T6437 13614-13621 VBD denotes rescued
T6438 13622-13625 DT denotes the
T6439 13626-13631 JJ denotes early
T6440 13632-13638 NN denotes defect
T6441 13639-13641 IN denotes in
T6442 13642-13647 JJ denotes IFN-γ
T6443 13648-13658 NN denotes production
T6444 13659-13667 VBD denotes observed
T6445 13668-13670 IN denotes in
T6446 13671-13676 NNP denotes T-bet
T6447 13677-13686 JJ denotes deficient
T6448 13687-13690 NNP denotes CD8
T6449 13690-13691 CD denotes +
T6450 13692-13693 NNP denotes T
T6451 13694-13699 NNS denotes cells
T6452 13700-13701 -LRB- denotes (
T6453 13701-13705 NN denotes Fig.
T6454 13706-13707 CD denotes 2
T6455 13708-13709 NNP denotes D
T6456 13709-13710 -RRB- denotes )
T6457 13710-13711 . denotes .
T6458 13712-13719 RB denotes However
T6459 13719-13720 , denotes ,
T6460 13721-13728 NN denotes Eo-VP16
T6461 13729-13732 VBD denotes did
T6462 13733-13736 RB denotes not
T6463 13737-13743 VB denotes induce
T6464 13744-13748 NNP denotes GzmB
T6465 13749-13753 NNP denotes mRNA
T6466 13754-13764 NN denotes expression
T6467 13765-13767 IN denotes in
T6468 13768-13774 CC denotes either
T6469 13775-13777 NNP denotes WT
T6470 13778-13780 CC denotes or
T6471 13781-13786 NNP denotes T-bet
T6472 13787-13796 JJ denotes deficient
T6473 13797-13802 NNS denotes cells
T6474 13802-13803 : denotes ;
T6475 13804-13808 RB denotes thus
T6476 13808-13809 , denotes ,
T6477 13810-13813 DT denotes the
T6478 13814-13821 JJ denotes partial
T6479 13822-13827 NN denotes T-bet
T6480 13828-13838 NN denotes dependence
T6481 13839-13841 IN denotes of
T6482 13842-13846 NNP denotes GzmB
T6483 13847-13851 NNP denotes mRNA
T6484 13852-13862 NN denotes expression
T6485 13863-13866 MD denotes can
T6486 13866-13869 RB denotes not
T6487 13870-13872 VB denotes be
T6488 13873-13884 VBN denotes compensated
T6489 13885-13888 IN denotes for
T6490 13889-13891 IN denotes by
T6491 13892-13899 NN denotes Eo-VP16
T6492 13899-13900 . denotes .
T7997 13902-13907 JJ denotes Runx3
T7998 13908-13916 NNS denotes controls
T7999 13917-13925 JJ denotes multiple
T8000 13926-13933 NNS denotes aspects
T8001 13934-13936 IN denotes of
T8002 13937-13940 DT denotes the
T8003 13941-13944 NNP denotes CTL
T8004 13945-13960 NN denotes differentiation
T8005 13961-13968 NN denotes program
T8006 13968-13969 , denotes ,
T8007 13970-13972 IN denotes in
T8008 13973-13977 NN denotes part
T8009 13978-13985 IN denotes through
T8010 13986-13995 NN denotes induction
T8011 13996-13998 IN denotes of
T8012 13999-14004 NNP denotes Eomes
T8013 14005-14012 IN denotes Because
T8014 14013-14018 NNP denotes Runx3
T8015 14019-14021 VBZ denotes is
T8016 14022-14028 RB denotes highly
T8017 14029-14038 VBN denotes expressed
T8018 14039-14041 IN denotes in
T8019 14042-14052 JJ denotes peripheral
T8020 14053-14056 NNP denotes CD8
T8021 14056-14057 CD denotes +
T8022 14058-14059 NNP denotes T
T8023 14060-14065 NNS denotes cells
T8024 14065-14066 , denotes ,
T8025 14067-14070 CC denotes and
T8026 14071-14078 IN denotes because
T8027 14079-14081 IN denotes of
T8028 14082-14085 DT denotes the
T8029 14086-14097 JJ denotes T-bet-Runx3
T8030 14098-14109 NN denotes cooperation
T8031 14110-14112 PRP denotes we
T8032 14113-14121 VBD denotes observed
T8033 14122-14129 RBR denotes earlier
T8034 14130-14132 IN denotes in
T8035 14133-14136 CD denotes CD4
T8036 14136-14137 CD denotes +
T8037 14138-14139 NNP denotes T
T8038 14140-14145 NNS denotes cells
T8039 14146-14147 -LRB- denotes (
T8040 14147-14149 CD denotes 15
T8041 14149-14150 -RRB- denotes )
T8042 14150-14151 , denotes ,
T8043 14152-14154 PRP denotes we
T8044 14155-14163 VBD denotes examined
T8045 14164-14167 DT denotes the
T8046 14168-14172 NN denotes role
T8047 14173-14175 IN denotes of
T8048 14176-14181 NNP denotes Runx3
T8049 14182-14184 IN denotes in
T8050 14185-14193 NN denotes effector
T8051 14194-14197 NNP denotes CTL
T8052 14198-14213 NN denotes differentiation
T8053 14213-14214 . denotes .
T8054 14215-14217 PRP denotes We
T8055 14218-14226 VBD denotes isolated
T8056 14227-14230 NNP denotes CD8
T8057 14230-14231 NNP denotes +
T8058 14232-14233 NNP denotes T
T8059 14234-14239 NNS denotes cells
T8060 14240-14244 IN denotes from
T8061 14245-14250 JJ denotes Runx3
T8062 14250-14251 NN denotes −
T8063 14251-14252 NN denotes /
T8064 14252-14253 NN denotes −
T8065 14254-14255 -LRB- denotes (
T8066 14255-14257 NNP denotes KO
T8067 14257-14258 -RRB- denotes )
T8068 14259-14263 NNS denotes mice
T8069 14264-14266 IN denotes of
T8070 14267-14270 DT denotes the
T8071 14271-14278 JJ denotes outbred
T8072 14279-14282 NNP denotes ICR
T8073 14283-14293 NN denotes background
T8074 14294-14297 CC denotes and
T8075 14298-14303 PRP$ denotes their
T8076 14304-14306 NNP denotes WT
T8077 14307-14312 NNP denotes Runx3
T8078 14312-14313 VBD denotes +
T8079 14313-14314 CD denotes /
T8080 14314-14315 NN denotes +
T8081 14316-14327 NNS denotes littermates
T8082 14328-14330 IN denotes by
T8083 14331-14339 JJ denotes positive
T8084 14340-14349 NN denotes selection
T8085 14350-14354 IN denotes with
T8086 14355-14363 JJ denotes anti-CD8
T8087 14364-14372 JJ denotes magnetic
T8088 14373-14378 NNS denotes beads
T8089 14379-14380 -LRB- denotes (
T8090 14380-14384 NNS denotes Figs
T8091 14384-14385 . denotes .
T8092 14386-14388 CD denotes S1
T8093 14389-14392 CC denotes and
T8094 14393-14395 CD denotes S2
T8095 14395-14396 , denotes ,
T8096 14397-14406 JJ denotes available
T8097 14407-14409 IN denotes at
T8098 14410-14462 NNP denotes http://www.jem.org/cgi/content/full/jem.20081242/DC1
T8099 14462-14463 -RRB- denotes )
T8100 14463-14464 . denotes .
T8101 14465-14475 RB denotes Strikingly
T8102 14475-14476 , denotes ,
T8103 14477-14482 JJ denotes Runx3
T8104 14482-14483 FW denotes −
T8105 14483-14484 FW denotes /
T8106 14484-14485 FW denotes −
T8107 14486-14489 FW denotes CD8
T8108 14489-14490 FW denotes +
T8109 14491-14492 FW denotes T
T8110 14493-14498 NNS denotes cells
T8111 14499-14503 VBD denotes were
T8112 14504-14512 RB denotes strongly
T8113 14513-14521 VBN denotes impaired
T8114 14522-14524 IN denotes in
T8115 14525-14530 PRP$ denotes their
T8116 14531-14538 NN denotes ability
T8117 14539-14541 TO denotes to
T8118 14542-14555 VB denotes differentiate
T8119 14556-14560 IN denotes into
T8120 14561-14569 NN denotes effector
T8121 14570-14574 NNS denotes CTLs
T8122 14574-14575 , denotes ,
T8123 14576-14578 IN denotes as
T8124 14579-14585 VBN denotes judged
T8125 14586-14588 IN denotes by
T8126 14589-14599 NN denotes expression
T8127 14600-14602 IN denotes of
T8128 14603-14611 NN denotes perforin
T8129 14611-14612 , denotes ,
T8130 14613-14621 NN denotes granzyme
T8131 14622-14623 NNP denotes B
T8132 14623-14624 , denotes ,
T8133 14625-14628 CC denotes and
T8134 14629-14634 NNP denotes IFN-γ
T8135 14635-14636 -LRB- denotes (
T8136 14636-14640 NNP denotes Fig.
T8137 14641-14642 CD denotes 3
T8138 14642-14643 -RRB- denotes )
T8139 14643-14644 . denotes .
T8140 14645-14653 VBN denotes Compared
T8141 14654-14658 IN denotes with
T8142 14659-14661 NNP denotes WT
T8143 14662-14663 NNP denotes T
T8144 14664-14669 NNS denotes cells
T8145 14669-14670 , denotes ,
T8146 14671-14679 NN denotes perforin
T8147 14680-14684 NNP denotes mRNA
T8148 14685-14688 CC denotes and
T8149 14689-14696 NN denotes protein
T8150 14697-14707 NN denotes expression
T8151 14708-14712 VBD denotes were
T8152 14713-14724 RB denotes essentially
T8153 14725-14737 JJ denotes undetectable
T8154 14738-14740 IN denotes in
T8155 14741-14746 NNP denotes Runx3
T8156 14746-14747 NNP denotes −
T8157 14747-14748 VBD denotes /
T8158 14748-14749 CD denotes −
T8159 14750-14751 NNP denotes T
T8160 14752-14757 NNS denotes cells
T8161 14758-14760 IN denotes at
T8162 14761-14764 NN denotes day
T8163 14765-14766 CD denotes 6
T8164 14767-14769 IN denotes of
T8165 14770-14777 NN denotes culture
T8166 14778-14779 -LRB- denotes (
T8167 14779-14783 JJ denotes Fig.
T8168 14784-14785 CD denotes 3
T8169 14785-14786 , denotes ,
T8170 14787-14788 DT denotes A
T8171 14789-14792 CC denotes and
T8172 14793-14794 NNP denotes B
T8173 14794-14795 -RRB- denotes )
T8174 14795-14796 . denotes .
T8175 14797-14802 JJ denotes Runx3
T8176 14802-14803 FW denotes −
T8177 14803-14804 FW denotes /
T8178 14804-14805 FW denotes −
T8179 14806-14807 FW denotes T
T8180 14808-14813 NNS denotes cells
T8181 14814-14818 RB denotes also
T8182 14819-14822 VBD denotes had
T8183 14823-14825 DT denotes no
T8184 14826-14836 JJ denotes detectable
T8185 14837-14842 NNP denotes Eomes
T8186 14843-14853 NN denotes expression
T8187 14853-14854 : denotes ;
T8188 14855-14857 IN denotes in
T8189 14858-14866 NN denotes contrast
T8190 14866-14867 , denotes ,
T8191 14868-14873 NN denotes T-bet
T8192 14874-14884 NN denotes expression
T8193 14885-14888 VBD denotes was
T8194 14889-14899 VBN denotes unimpaired
T8195 14900-14901 -LRB- denotes (
T8196 14901-14905 JJ denotes Fig.
T8197 14906-14907 CD denotes 3
T8198 14908-14909 NN denotes A
T8199 14909-14910 -RRB- denotes )
T8200 14910-14911 . denotes .
T8201 14912-14923 RB denotes Furthermore
T8202 14923-14924 , denotes ,
T8203 14925-14930 NNP denotes Runx3
T8204 14931-14934 VBD denotes was
T8205 14935-14943 VBN denotes required
T8206 14944-14947 IN denotes for
T8207 14948-14955 JJ denotes maximal
T8208 14956-14966 NN denotes production
T8209 14967-14969 IN denotes of
T8210 14970-14975 NNP denotes IFN-γ
T8211 14975-14976 , denotes ,
T8212 14977-14980 CC denotes but
T8213 14981-14984 RB denotes not
T8214 14985-14988 NNP denotes TNF
T8215 14989-14991 CC denotes or
T8216 14992-14996 NNP denotes IL-2
T8217 14996-14997 , denotes ,
T8218 14998-15000 IN denotes by
T8219 15001-15004 CD denotes CD8
T8220 15004-15005 CD denotes +
T8221 15006-15007 NNP denotes T
T8222 15008-15013 NNS denotes cells
T8223 15014-15026 VBD denotes restimulated
T8224 15027-15029 IN denotes at
T8225 15030-15033 NN denotes day
T8226 15034-15035 CD denotes 6
T8227 15036-15037 -LRB- denotes (
T8228 15037-15041 NN denotes Fig.
T8229 15042-15043 CD denotes 3
T8230 15044-15045 NNP denotes C
T8231 15045-15046 -RRB- denotes )
T8232 15046-15047 . denotes .
T8233 16186-16188 PRP denotes We
T8234 16189-16199 RB denotes previously
T8235 16200-16208 VBD denotes reported
T8236 16209-16213 IN denotes that
T8237 16214-16217 CD denotes Th1
T8238 16218-16222 NN denotes cell
T8239 16223-16238 NN denotes differentiation
T8240 16239-16242 VBD denotes was
T8241 16243-16252 VBN denotes regulated
T8242 16253-16260 IN denotes through
T8243 16261-16262 DT denotes a
T8244 16263-16275 JJ denotes feed-forward
T8245 16276-16280 NN denotes loop
T8246 16281-16283 IN denotes in
T8247 16284-16289 WDT denotes which
T8248 16290-16295 NNP denotes T-bet
T8249 16296-16298 VBZ denotes is
T8250 16299-16311 JJ denotes up-regulated
T8251 16312-16317 RB denotes early
T8252 16318-16321 CC denotes and
T8253 16322-16329 VBZ denotes induces
T8254 16330-16335 NNP denotes Runx3
T8255 16335-16336 , denotes ,
T8256 16337-16342 IN denotes after
T8257 16343-16348 WDT denotes which
T8258 16349-16354 NNP denotes T-bet
T8259 16355-16358 CC denotes and
T8260 16359-16364 NNP denotes Runx3
T8261 16365-16374 VBP denotes cooperate
T8262 16375-16377 TO denotes to
T8263 16378-16384 VB denotes induce
T8264 16385-16390 NNP denotes IFN-γ
T8265 16391-16394 CC denotes and
T8266 16395-16402 NN denotes silence
T8267 16403-16407 NN denotes IL-4
T8268 16407-16408 , denotes ,
T8269 16409-16413 RB denotes thus
T8270 16414-16423 VBG denotes promoting
T8271 16424-16430 JJ denotes stable
T8272 16431-16446 NN denotes differentiation
T8273 16447-16453 IN denotes toward
T8274 16454-16457 DT denotes the
T8275 16458-16461 JJ denotes Th1
T8276 16462-16469 NN denotes lineage
T8277 16470-16471 -LRB- denotes (
T8278 16471-16473 CD denotes 15
T8279 16473-16474 , denotes ,
T8280 16475-16477 CD denotes 22
T8281 16477-16478 -RRB- denotes )
T8282 16478-16479 . denotes .
T8283 16480-16487 IN denotes Because
T8284 16488-16489 -LRB- denotes (
T8285 16489-16490 DT denotes a
T8286 16490-16491 -RRB- denotes )
T8287 16492-16497 JJ denotes Runx3
T8288 16498-16506 VBD denotes appeared
T8289 16507-16516 JJ denotes necessary
T8290 16517-16520 IN denotes for
T8291 16521-16526 NNP denotes Eomes
T8292 16527-16536 NN denotes induction
T8293 16537-16538 -LRB- denotes (
T8294 16538-16542 JJ denotes Fig.
T8295 16543-16544 CD denotes 3
T8296 16545-16546 NN denotes A
T8297 16546-16547 -RRB- denotes )
T8298 16547-16548 , denotes ,
T8299 16549-16550 -LRB- denotes (
T8300 16550-16551 NN denotes b
T8301 16551-16552 -RRB- denotes )
T8302 16553-16556 DT denotes the
T8303 16557-16565 NNS denotes kinetics
T8304 16566-16568 IN denotes of
T8305 16569-16574 NNP denotes Eomes
T8306 16575-16585 NN denotes expression
T8307 16586-16596 VBD denotes paralleled
T8308 16597-16602 DT denotes those
T8309 16603-16605 IN denotes of
T8310 16606-16614 NN denotes perforin
T8311 16615-16625 NN denotes expression
T8312 16626-16627 -LRB- denotes (
T8313 16627-16631 FW denotes Fig.
T8314 16632-16633 LS denotes 2
T8315 16633-16634 -RRB- denotes )
T8316 16634-16635 , denotes ,
T8317 16636-16639 CC denotes and
T8318 16640-16641 -LRB- denotes (
T8319 16641-16642 NN denotes c
T8320 16642-16643 -RRB- denotes )
T8321 16644-16658 NN denotes overexpression
T8322 16659-16661 IN denotes of
T8323 16662-16669 NN denotes Eo-VP16
T8324 16670-16672 IN denotes in
T8325 16673-16679 CC denotes either
T8326 16680-16682 NNP denotes WT
T8327 16683-16685 CC denotes or
T8328 16686-16691 NNP denotes T-bet
T8329 16692-16701 JJ denotes deficient
T8330 16702-16703 NNP denotes T
T8331 16704-16709 NNS denotes cells
T8332 16710-16713 VBD denotes led
T8333 16714-16716 TO denotes to
T8334 16717-16719 DT denotes an
T8335 16720-16728 NN denotes increase
T8336 16729-16731 IN denotes in
T8337 16732-16736 DT denotes both
T8338 16737-16745 NN denotes perforin
T8339 16746-16749 CC denotes and
T8340 16750-16755 JJ denotes IFN-γ
T8341 16756-16766 NN denotes expression
T8342 16767-16768 -LRB- denotes (
T8343 16768-16772 JJ denotes Fig.
T8344 16773-16774 CD denotes 2
T8345 16774-16775 , denotes ,
T8346 16776-16777 NNP denotes B
T8347 16778-16781 CC denotes and
T8348 16782-16783 NNP denotes D
T8349 16783-16784 -RRB- denotes )
T8350 16784-16785 , denotes ,
T8351 16786-16788 PRP denotes we
T8352 16789-16794 VBD denotes asked
T8353 16795-16802 IN denotes whether
T8354 16803-16806 NNP denotes CTL
T8355 16807-16822 NN denotes differentiation
T8356 16823-16826 VBD denotes was
T8357 16827-16831 RB denotes also
T8358 16832-16843 RB denotes potentially
T8359 16844-16853 VBN denotes regulated
T8360 16854-16856 IN denotes by
T8361 16857-16858 DT denotes a
T8362 16859-16871 JJ denotes feed-forward
T8363 16872-16876 NN denotes loop
T8364 16877-16886 VBG denotes involving
T8365 16887-16892 DT denotes these
T8366 16893-16897 JJ denotes same
T8367 16898-16901 CD denotes two
T8368 16902-16909 NNS denotes classes
T8369 16910-16912 IN denotes of
T8370 16913-16917 NNP denotes Runx
T8371 16918-16921 CC denotes and
T8372 16922-16927 NNP denotes T-box
T8373 16928-16941 NN denotes transcription
T8374 16942-16949 NNS denotes factors
T8375 16949-16950 . denotes .
T8376 16951-16963 RB denotes Specifically
T8377 16963-16964 , denotes ,
T8378 16965-16967 PRP denotes we
T8379 16968-16973 VBD denotes asked
T8380 16974-16981 IN denotes whether
T8381 16982-16987 NNP denotes Runx3
T8382 16987-16988 , denotes ,
T8383 16989-16994 WDT denotes which
T8384 16995-16998 VBD denotes was
T8385 16999-17008 JJ denotes necessary
T8386 17009-17012 IN denotes for
T8387 17013-17018 NNP denotes Eomes
T8388 17019-17028 NN denotes induction
T8389 17028-17029 , denotes ,
T8390 17030-17034 RB denotes then
T8391 17035-17045 VBD denotes cooperated
T8392 17046-17050 IN denotes with
T8393 17051-17056 NNP denotes Eomes
T8394 17057-17059 TO denotes to
T8395 17060-17068 VB denotes regulate
T8396 17069-17082 NN denotes transcription
T8397 17083-17085 IN denotes of
T8398 17086-17089 DT denotes the
T8399 17090-17098 NN denotes effector
T8400 17099-17102 NNP denotes CTL
T8401 17103-17110 NNS denotes markers
T8402 17111-17119 VBP denotes perforin
T8403 17119-17120 , denotes ,
T8404 17121-17126 NNP denotes IFN-γ
T8405 17126-17127 , denotes ,
T8406 17128-17131 CC denotes and
T8407 17132-17140 NN denotes granzyme
T8408 17141-17143 NNP denotes B.
T8409 17144-17146 TO denotes To
T8410 17147-17151 VB denotes test
T8411 17152-17156 DT denotes this
T8412 17157-17167 NN denotes hypothesis
T8413 17167-17168 , denotes ,
T8414 17169-17171 PRP denotes we
T8415 17172-17176 VBD denotes used
T8416 17177-17186 NN denotes chromatin
T8417 17187-17206 NN denotes immunoprecipitation
T8418 17207-17208 -LRB- denotes (
T8419 17208-17212 NNP denotes ChIP
T8420 17212-17213 -RRB- denotes )
T8421 17214-17220 VBZ denotes assays
T8422 17221-17223 TO denotes to
T8423 17224-17227 VB denotes ask
T8424 17228-17235 IN denotes whether
T8425 17236-17241 NNP denotes Eomes
T8426 17242-17245 CC denotes and
T8427 17246-17251 NNP denotes Runx3
T8428 17252-17257 VBD denotes bound
T8429 17258-17268 JJ denotes regulatory
T8430 17269-17276 NNS denotes regions
T8431 17277-17279 IN denotes of
T8432 17280-17283 DT denotes the
T8433 17284-17288 NNP denotes Prf1
T8434 17288-17289 , denotes ,
T8435 17290-17294 NNP denotes Ifng
T8436 17294-17295 , denotes ,
T8437 17296-17299 CC denotes and
T8438 17300-17304 NNP denotes Gzmb
T8439 17305-17310 NNS denotes genes
T8440 17311-17312 -LRB- denotes (
T8441 17312-17316 NN denotes Fig.
T8442 17317-17318 CD denotes 3
T8443 17319-17320 NNP denotes D
T8444 17320-17321 -RRB- denotes )
T8445 17321-17322 . denotes .
T8446 17323-17327 DT denotes Both
T8447 17328-17336 NNS denotes proteins
T8448 17337-17347 VBN denotes associated
T8449 17348-17352 IN denotes with
T8450 17353-17357 NN denotes gene
T8451 17358-17368 JJ denotes regulatory
T8452 17369-17376 NNS denotes regions
T8453 17377-17379 IN denotes in
T8454 17380-17394 JJ denotes differentiated
T8455 17395-17399 NNS denotes CTLs
T8456 17399-17400 . denotes .
T8457 17401-17406 JJ denotes Runx3
T8458 17407-17412 VBN denotes bound
T8459 17413-17415 TO denotes to
T8460 17416-17419 DT denotes the
T8461 17420-17424 NNP denotes Prf1
T8462 17425-17428 CC denotes and
T8463 17429-17433 NNP denotes Gzmb
T8464 17434-17447 NN denotes transcription
T8465 17448-17453 NN denotes start
T8466 17454-17459 NNS denotes sites
T8467 17460-17461 -LRB- denotes (
T8468 17461-17464 NNP denotes TSS
T8469 17464-17465 -RRB- denotes )
T8470 17465-17466 : denotes ;
T8471 17467-17469 TO denotes to
T8472 17470-17471 DT denotes a
T8473 17472-17477 VBN denotes known
T8474 17478-17482 NN denotes IL-2
T8475 17483-17493 JJ denotes responsive
T8476 17494-17502 NN denotes enhancer
T8477 17503-17510 VBN denotes located
T8478 17511-17515 IN denotes near
T8479 17516-17517 CD denotes −
T8480 17517-17518 CD denotes 1
T8481 17519-17521 NN denotes kb
T8482 17522-17524 IN denotes of
T8483 17525-17528 DT denotes the
T8484 17529-17533 NNP denotes Prf1
T8485 17534-17538 NN denotes gene
T8486 17539-17540 -LRB- denotes (
T8487 17540-17542 CD denotes 23
T8488 17542-17543 -RRB- denotes )
T8489 17543-17544 : denotes ;
T8490 17545-17547 TO denotes to
T8491 17548-17551 DT denotes the
T8492 17552-17558 JJ denotes distal
T8493 17559-17571 JJ denotes CTL-specific
T8494 17572-17577 NNP denotes DNase
T8495 17578-17579 PRP denotes I
T8496 17580-17594 VBP denotes hypersensitive
T8497 17595-17599 NN denotes site
T8498 17600-17601 CD denotes 9
T8499 17602-17604 IN denotes in
T8500 17605-17608 DT denotes the
T8501 17609-17613 JJ denotes Prf1
T8502 17614-17619 NN denotes locus
T8503 17620-17621 -LRB- denotes (
T8504 17621-17623 CD denotes 24
T8505 17623-17624 -RRB- denotes )
T8506 17624-17625 : denotes ;
T8507 17626-17628 TO denotes to
T8508 17629-17632 DT denotes the
T8509 17633-17637 NNP denotes Ifng
T8510 17638-17646 NN denotes promoter
T8511 17647-17651 IN denotes near
T8512 17652-17655 DT denotes the
T8513 17656-17659 NNP denotes TSS
T8514 17659-17660 , denotes ,
T8515 17661-17663 IN denotes as
T8516 17664-17674 RB denotes previously
T8517 17675-17683 VBN denotes reported
T8518 17684-17687 IN denotes for
T8519 17688-17691 JJ denotes Th1
T8520 17692-17697 NNS denotes cells
T8521 17698-17699 -LRB- denotes (
T8522 17699-17701 CD denotes 10
T8523 17701-17702 -RRB- denotes )
T8524 17702-17703 : denotes ;
T8525 17704-17707 CC denotes and
T8526 17708-17710 TO denotes to
T8527 17711-17718 JJ denotes several
T8528 17719-17724 NNP denotes DNase
T8529 17725-17726 PRP denotes I
T8530 17727-17741 VBP denotes hypersensitive
T8531 17742-17747 NNS denotes sites
T8532 17748-17750 IN denotes in
T8533 17751-17754 DT denotes the
T8534 17755-17759 NNP denotes Ifng
T8535 17760-17765 NN denotes locus
T8536 17766-17767 -LRB- denotes (
T8537 17767-17771 JJ denotes Fig.
T8538 17772-17773 CD denotes 3
T8539 17774-17775 NNP denotes D
T8540 17776-17779 CC denotes and
T8541 17780-17783 RB denotes not
T8542 17784-17792 VBN denotes depicted
T8543 17792-17793 -RRB- denotes )
T8544 17794-17795 -LRB- denotes (
T8545 17795-17797 CD denotes 25
T8546 17797-17798 -RRB- denotes )
T8547 17798-17799 . denotes .
T8548 17800-17805 NNS denotes Eomes
T8549 17806-17811 VBN denotes bound
T8550 17812-17821 RB denotes primarily
T8551 17822-17824 TO denotes to
T8552 17825-17828 DT denotes the
T8553 17829-17833 NNP denotes Prf1
T8554 17834-17837 NNP denotes TSS
T8555 17838-17841 CC denotes and
T8556 17842-17845 DT denotes the
T8557 17846-17847 NN denotes −
T8558 17847-17848 CD denotes 1
T8559 17849-17851 NN denotes kb
T8560 17852-17860 NN denotes enhancer
T8561 17860-17861 : denotes ;
T8562 17862-17866 DT denotes this
T8563 17867-17874 JJ denotes binding
T8564 17875-17878 VBD denotes was
T8565 17879-17892 RB denotes substantially
T8566 17893-17900 JJR denotes greater
T8567 17901-17905 IN denotes than
T8568 17906-17910 DT denotes that
T8569 17911-17919 VBD denotes observed
T8570 17920-17922 IN denotes at
T8571 17923-17926 DT denotes the
T8572 17927-17935 NN denotes promoter
T8573 17936-17938 IN denotes of
T8574 17939-17942 DT denotes the
T8575 17943-17948 JJ denotes Il2rb
T8576 17949-17953 NN denotes gene
T8577 17953-17954 , denotes ,
T8578 17955-17956 DT denotes a
T8579 17957-17962 VBN denotes known
T8580 17963-17969 JJ denotes direct
T8581 17970-17976 NN denotes target
T8582 17977-17979 IN denotes of
T8583 17980-17985 NNP denotes Eomes
T8584 17986-17987 -LRB- denotes (
T8585 17987-17988 CD denotes 8
T8586 17988-17989 -RRB- denotes )
T8587 17989-17990 , denotes ,
T8588 17991-17994 CC denotes and
T8589 17995-18005 JJ denotes comparable
T8590 18006-18008 TO denotes to
T8591 18009-18013 DT denotes that
T8592 18014-18022 VBD denotes observed
T8593 18023-18025 IN denotes at
T8594 18026-18029 DT denotes the
T8595 18030-18034 NNP denotes Ifng
T8596 18035-18038 NNP denotes TSS
T8597 18038-18039 , denotes ,
T8598 18040-18041 DT denotes a
T8599 18042-18047 VBN denotes known
T8600 18048-18054 NN denotes target
T8601 18055-18057 IN denotes of
T8602 18058-18063 NN denotes T-box
T8603 18064-18072 NNS denotes proteins
T8604 18073-18075 IN denotes in
T8605 18076-18080 DT denotes both
T8606 18081-18084 CD denotes Th1
T8607 18085-18088 CC denotes and
T8608 18089-18092 CD denotes CD8
T8609 18092-18093 CD denotes +
T8610 18094-18095 NNP denotes T
T8611 18096-18101 NNS denotes cells
T8612 18102-18103 -LRB- denotes (
T8613 18103-18107 NN denotes Fig.
T8614 18108-18109 CD denotes 3
T8615 18110-18111 NNP denotes D
T8616 18111-18112 -RRB- denotes )
T8617 18113-18114 -LRB- denotes (
T8618 18114-18116 CD denotes 17
T8619 18116-18117 -RRB- denotes )
T8620 18117-18118 . denotes .
T8621 18119-18121 TO denotes To
T8622 18122-18131 VB denotes determine
T8623 18132-18139 IN denotes whether
T8624 18140-18145 JJ denotes Runx3
T8625 18146-18156 VBN denotes controlled
T8626 18157-18160 DT denotes the
T8627 18161-18171 NN denotes expression
T8628 18172-18174 IN denotes of
T8629 18175-18178 NNP denotes CTL
T8630 18179-18187 NN denotes effector
T8631 18188-18193 NNS denotes genes
T8632 18194-18201 IN denotes through
T8633 18202-18205 PRP$ denotes its
T8634 18206-18215 NN denotes induction
T8635 18216-18218 IN denotes of
T8636 18219-18224 NNP denotes Eomes
T8637 18224-18225 , denotes ,
T8638 18226-18228 PRP denotes we
T8639 18229-18241 RB denotes retrovirally
T8640 18242-18251 VBD denotes expressed
T8641 18252-18257 NNP denotes Runx3
T8642 18258-18261 CC denotes and
T8643 18262-18269 NNP denotes Eo-VP16
T8644 18270-18272 IN denotes in
T8645 18273-18276 NNP denotes CD8
T8646 18276-18277 NNP denotes +
T8647 18278-18279 NNP denotes T
T8648 18280-18285 NNS denotes cells
T8649 18286-18290 IN denotes from
T8650 18291-18296 JJ denotes Runx3
T8651 18296-18297 NN denotes −
T8652 18297-18298 NN denotes /
T8653 18298-18299 NN denotes −
T8654 18300-18304 NNS denotes mice
T8655 18304-18305 . denotes .
T8656 18306-18313 IN denotes Because
T8657 18314-18316 IN denotes of
T8658 18317-18320 DT denotes the
T8659 18321-18328 JJ denotes limited
T8660 18329-18335 NN denotes number
T8661 18336-18338 IN denotes of
T8662 18339-18342 CD denotes CD8
T8663 18342-18343 CD denotes +
T8664 18344-18345 NNP denotes T
T8665 18346-18351 NNS denotes cells
T8666 18352-18354 IN denotes in
T8667 18355-18360 DT denotes these
T8668 18361-18365 NNS denotes mice
T8669 18365-18366 , denotes ,
T8670 18367-18370 CC denotes and
T8671 18371-18378 IN denotes because
T8672 18379-18381 PRP denotes we
T8673 18382-18385 VBD denotes saw
T8674 18386-18388 DT denotes no
T8675 18389-18399 NN denotes difference
T8676 18400-18407 IN denotes between
T8677 18408-18413 NNP denotes Runx3
T8678 18413-18414 FW denotes −
T8679 18414-18415 FW denotes /
T8680 18415-18416 FW denotes −
T8681 18417-18420 FW denotes CD8
T8682 18420-18421 FW denotes +
T8683 18421-18424 FW denotes CD4
T8684 18424-18425 FW denotes −
T8685 18426-18428 NNP denotes SP
T8686 18429-18432 CC denotes and
T8687 18433-18436 NNP denotes CD8
T8688 18436-18437 NNP denotes +
T8689 18437-18440 NNP denotes CD4
T8690 18440-18441 NNP denotes +
T8691 18442-18444 NNP denotes DP
T8692 18445-18450 NNS denotes cells
T8693 18451-18453 IN denotes in
T8694 18454-18457 PRP$ denotes our
T8695 18458-18466 JJ denotes previous
T8696 18467-18478 NNS denotes experiments
T8697 18478-18479 , denotes ,
T8698 18480-18482 PRP denotes we
T8699 18483-18487 VBD denotes used
T8700 18488-18493 JJ denotes total
T8701 18494-18499 JJ denotes Runx3
T8702 18499-18500 NN denotes −
T8703 18500-18501 FW denotes /
T8704 18501-18502 FW denotes −
T8705 18503-18506 FW denotes CD8
T8706 18506-18507 FW denotes +
T8707 18508-18509 FW denotes T
T8708 18510-18515 NNS denotes cells
T8709 18516-18523 IN denotes without
T8710 18524-18531 JJ denotes further
T8711 18532-18545 NN denotes fractionation
T8712 18546-18548 IN denotes as
T8713 18549-18559 NNS denotes recipients
T8714 18560-18563 IN denotes for
T8715 18564-18574 JJ denotes retroviral
T8716 18575-18587 NN denotes transduction
T8717 18587-18588 . denotes .
T8718 18589-18603 NN denotes Reconstitution
T8719 18604-18606 IN denotes of
T8720 18607-18612 NNP denotes Runx3
T8721 18612-18613 FW denotes −
T8722 18613-18614 FW denotes /
T8723 18614-18615 FW denotes −
T8724 18616-18619 FW denotes CD8
T8725 18619-18620 FW denotes +
T8726 18621-18622 FW denotes T
T8727 18623-18628 NNS denotes cells
T8728 18629-18633 IN denotes with
T8729 18634-18639 JJ denotes Runx3
T8730 18640-18648 VBN denotes restored
T8731 18649-18659 NN denotes expression
T8732 18660-18662 IN denotes of
T8733 18663-18668 NNP denotes Eomes
T8734 18669-18671 RB denotes as
T8735 18672-18676 RB denotes well
T8736 18677-18679 IN denotes as
T8737 18680-18688 NN denotes perforin
T8738 18688-18689 , denotes ,
T8739 18690-18698 NN denotes granzyme
T8740 18699-18700 NNP denotes B
T8741 18700-18701 , denotes ,
T8742 18702-18705 CC denotes and
T8743 18706-18711 NNP denotes IFN-γ
T8744 18712-18713 -LRB- denotes (
T8745 18713-18717 NNP denotes Fig.
T8746 18718-18719 CD denotes 4
T8747 18719-18720 , denotes ,
T8748 18721-18722 DT denotes A
T8749 18723-18726 CC denotes and
T8750 18727-18728 NNP denotes B
T8751 18728-18729 -RRB- denotes )
T8752 18729-18730 . denotes .
T8753 18731-18733 IN denotes In
T8754 18734-18742 NN denotes addition
T8755 18742-18743 , denotes ,
T8756 18744-18749 NNP denotes Runx3
T8757 18749-18750 VBD denotes −
T8758 18750-18751 CD denotes /
T8759 18751-18752 CD denotes −
T8760 18753-18754 NNP denotes T
T8761 18755-18760 NNS denotes cells
T8762 18761-18767 VBD denotes showed
T8763 18768-18769 DT denotes a
T8764 18770-18782 JJ denotes compensatory
T8765 18783-18796 NN denotes up-regulation
T8766 18797-18799 IN denotes of
T8767 18800-18805 NNP denotes Runx1
T8768 18805-18806 , denotes ,
T8769 18807-18812 WDT denotes which
T8770 18813-18816 VBD denotes was
T8771 18817-18827 VBN denotes suppressed
T8772 18828-18832 IN denotes upon
T8773 18833-18847 NN denotes reconstitution
T8774 18848-18852 IN denotes with
T8775 18853-18858 NNP denotes Runx3
T8776 18858-18859 , denotes ,
T8777 18860-18870 VBG denotes indicating
T8778 18871-18875 IN denotes that
T8779 18876-18881 NNP denotes Runx1
T8780 18882-18884 VBZ denotes is
T8781 18885-18886 DT denotes a
T8782 18887-18893 NN denotes target
T8783 18894-18896 IN denotes of
T8784 18897-18907 NN denotes repression
T8785 18908-18910 IN denotes by
T8786 18911-18916 NNP denotes Runx3
T8787 18916-18917 . denotes .
T8788 18918-18925 RB denotes Notably
T8789 18925-18926 , denotes ,
T8790 18927-18934 NN denotes Eo-VP16
T8791 18935-18938 VBD denotes did
T8792 18939-18942 RB denotes not
T8793 18943-18954 JJ denotes up-regulate
T8794 18955-18963 NN denotes perforin
T8795 18964-18974 NN denotes expression
T8796 18975-18979 WRB denotes when
T8797 18980-18989 VBN denotes expressed
T8798 18990-18992 IN denotes in
T8799 18993-18998 NNP denotes Runx3
T8800 18998-18999 NNP denotes −
T8801 18999-19000 VBD denotes /
T8802 19000-19001 CD denotes −
T8803 19002-19007 NNS denotes cells
T8804 19007-19008 , denotes ,
T8805 19009-19013 RB denotes even
T8806 19014-19020 IN denotes though
T8807 19021-19023 PRP denotes it
T8808 19024-19032 VBD denotes restored
T8809 19033-19036 DT denotes the
T8810 19037-19045 NN denotes capacity
T8811 19046-19048 TO denotes to
T8812 19049-19055 VB denotes induce
T8813 19056-19061 JJ denotes IFN-γ
T8814 19062-19072 NN denotes expression
T8815 19073-19077 IN denotes upon
T8816 19078-19081 NNP denotes TCR
T8817 19082-19095 NN denotes restimulation
T8818 19096-19097 -LRB- denotes (
T8819 19097-19101 JJ denotes Fig.
T8820 19102-19103 CD denotes 4
T8821 19103-19104 , denotes ,
T8822 19105-19106 DT denotes A
T8823 19107-19110 CC denotes and
T8824 19111-19112 NNP denotes B
T8825 19112-19113 -RRB- denotes )
T8826 19113-19114 . denotes .
T8827 19115-19119 DT denotes This
T8828 19120-19126 NN denotes result
T8829 19127-19135 VBZ denotes suggests
T8830 19136-19144 RB denotes strongly
T8831 19145-19149 IN denotes that
T8832 19150-19158 JJ denotes perforin
T8833 19159-19169 NN denotes expression
T8834 19170-19178 VBZ denotes requires
T8835 19179-19184 NNP denotes Runx3
T8836 19185-19188 CC denotes and
T8837 19189-19194 NNP denotes Eomes
T8838 19194-19195 . denotes .
T8839 20867-20869 IN denotes As
T8840 20870-20878 VBN denotes expected
T8841 20879-20883 IN denotes from
T8842 20884-20889 PRP$ denotes their
T8843 20890-20896 NN denotes defect
T8844 20897-20899 IN denotes in
T8845 20900-20908 NN denotes perforin
T8846 20909-20912 CC denotes and
T8847 20913-20921 NN denotes granzyme
T8848 20922-20923 NNP denotes B
T8849 20924-20934 NN denotes expression
T8850 20934-20935 , denotes ,
T8851 20936-20941 JJ denotes Runx3
T8852 20941-20942 FW denotes −
T8853 20942-20943 FW denotes /
T8854 20943-20944 FW denotes −
T8855 20945-20948 FW denotes CD8
T8856 20948-20949 FW denotes +
T8857 20950-20951 FW denotes T
T8858 20952-20957 NNS denotes cells
T8859 20958-20964 VBD denotes showed
T8860 20965-20974 JJ denotes defective
T8861 20975-20984 JJ denotes cytolytic
T8862 20985-20993 NN denotes activity
T8863 20994-20996 IN denotes in
T8864 20997-20998 DT denotes a
T8865 20999-21004 JJ denotes mixed
T8866 21005-21015 NN denotes lymphocyte
T8867 21016-21024 NN denotes reaction
T8868 21025-21026 -LRB- denotes (
T8869 21026-21028 CD denotes 12
T8870 21028-21029 -RRB- denotes )
T8871 21029-21030 . denotes .
T8872 21031-21038 RB denotes However
T8873 21038-21039 , denotes ,
T8874 21040-21054 JJ denotes TCR-stimulated
T8875 21055-21060 JJ denotes Runx3
T8876 21060-21061 NN denotes −
T8877 21061-21062 VBD denotes /
T8878 21062-21063 CD denotes −
T8879 21064-21067 CD denotes CD8
T8880 21067-21068 NN denotes +
T8881 21069-21074 NNS denotes cells
T8882 21075-21079 VBD denotes were
T8883 21080-21082 IN denotes as
T8884 21083-21092 JJ denotes effective
T8885 21093-21095 IN denotes as
T8886 21096-21098 NNP denotes WT
T8887 21099-21104 NNS denotes cells
T8888 21105-21107 IN denotes in
T8889 21108-21115 VBG denotes killing
T8890 21116-21121 NN denotes tumor
T8891 21122-21127 NNS denotes cells
T8892 21128-21130 IN denotes in
T8893 21131-21132 DT denotes a
T8894 21133-21143 VBN denotes redirected
T8895 21144-21147 NNP denotes CTL
T8896 21148-21153 NN denotes assay
T8897 21154-21155 -LRB- denotes (
T8898 21155-21157 CD denotes 12
T8899 21157-21158 -RRB- denotes )
T8900 21158-21159 . denotes .
T8901 21160-21171 RB denotes Furthermore
T8902 21171-21172 , denotes ,
T8903 21173-21176 NNP denotes CD8
T8904 21176-21177 NNP denotes +
T8905 21178-21183 NNS denotes cells
T8906 21184-21188 IN denotes from
T8907 21189-21192 DT denotes the
T8908 21193-21203 JJ denotes peritoneal
T8909 21204-21210 NN denotes cavity
T8910 21211-21213 IN denotes of
T8911 21214-21219 NNP denotes Runx3
T8912 21219-21220 NNP denotes −
T8913 21220-21221 VBD denotes /
T8914 21221-21222 CD denotes −
T8915 21223-21227 NNS denotes mice
T8916 21228-21237 VBN denotes immunized
T8917 21238-21242 IN denotes with
T8918 21243-21250 JJ denotes certain
T8919 21251-21256 NN denotes tumor
T8920 21257-21262 NNS denotes cells
T8921 21263-21274 RB denotes effectively
T8922 21275-21281 VBD denotes killed
T8923 21282-21287 DT denotes these
T8924 21288-21295 NNS denotes targets
T8925 21296-21297 -LRB- denotes (
T8926 21297-21299 CD denotes 13
T8927 21299-21300 -RRB- denotes )
T8928 21300-21301 . denotes .
T8929 21302-21311 RB denotes Therefore
T8930 21311-21312 , denotes ,
T8931 21313-21321 IN denotes although
T8932 21322-21332 NN denotes activation
T8933 21333-21335 IN denotes of
T8934 21336-21339 DT denotes the
T8935 21340-21357 NN denotes perforin/granzyme
T8936 21358-21359 NNP denotes B
T8937 21360-21369 NN denotes machinery
T8938 21370-21372 VBZ denotes is
T8939 21373-21382 JJ denotes defective
T8940 21383-21385 IN denotes in
T8941 21386-21391 NNP denotes Runx3
T8942 21391-21392 NNP denotes −
T8943 21392-21393 VBD denotes /
T8944 21393-21394 CD denotes −
T8945 21395-21398 CD denotes CD8
T8946 21398-21399 NN denotes +
T8947 21400-21405 NNS denotes cells
T8948 21405-21406 , denotes ,
T8949 21407-21412 DT denotes these
T8950 21413-21418 NNS denotes cells
T8951 21419-21422 VBP denotes are
T8952 21423-21426 RB denotes not
T8953 21427-21435 RB denotes entirely
T8954 21436-21442 JJ denotes devoid
T8955 21443-21445 IN denotes of
T8956 21446-21455 JJ denotes cytolytic
T8957 21456-21464 NN denotes activity
T8958 21465-21468 CC denotes and
T8959 21469-21474 MD denotes could
T8960 21475-21480 RB denotes still
T8961 21481-21492 RB denotes effectively
T8962 21493-21497 VB denotes kill
T8963 21498-21505 NNS denotes targets
T8964 21505-21506 , denotes ,
T8965 21507-21515 RB denotes possibly
T8966 21516-21518 IN denotes by
T8967 21519-21530 JJ denotes alternative
T8968 21531-21541 NNS denotes mechanisms
T8969 21542-21546 JJ denotes such
T8970 21547-21549 IN denotes as
T8971 21550-21553 DT denotes the
T8972 21554-21557 NNP denotes Fas
T8973 21558-21561 NNP denotes Fas
T8974 21562-21568 VBD denotes ligand
T8975 21569-21576 NN denotes pathway
T8976 21576-21577 . denotes .
T11912 21579-21584 JJ denotes Runx3
T11913 21585-21588 CC denotes and
T11914 21589-21594 JJ denotes T-box
T11915 21595-21602 NNS denotes factors
T11916 21603-21610 VBP denotes control
T11917 21611-21612 DT denotes a
T11918 21613-21620 JJ denotes complex
T11919 21621-21628 NN denotes program
T11920 21629-21631 IN denotes of
T11921 21632-21647 JJ denotes transcriptional
T11922 21648-21658 NN denotes regulation
T11923 21659-21665 IN denotes during
T11924 21666-21669 NNP denotes CTL
T11925 21670-21685 NN denotes differentiation
T11926 21686-21698 RB denotes Collectively
T11927 21698-21699 , denotes ,
T11928 21700-21705 DT denotes these
T11929 21706-21710 NNS denotes data
T11930 21711-21718 VBP denotes provide
T11931 21719-21727 NN denotes evidence
T11932 21728-21732 IN denotes that
T11933 21733-21738 NNP denotes Runx3
T11934 21738-21739 , denotes ,
T11935 21740-21748 RB denotes together
T11936 21749-21753 IN denotes with
T11937 21754-21759 JJ denotes T-box
T11938 21760-21767 NNS denotes factors
T11939 21767-21768 , denotes ,
T11940 21769-21781 VBZ denotes orchestrates
T11941 21782-21783 DT denotes a
T11942 21784-21791 JJ denotes complex
T11943 21792-21799 NN denotes program
T11944 21800-21802 IN denotes of
T11945 21803-21818 JJ denotes transcriptional
T11946 21819-21829 NN denotes regulation
T11947 21830-21832 IN denotes in
T11948 21833-21848 VBG denotes differentiating
T11949 21849-21852 NNP denotes CTL
T11950 21853-21854 -LRB- denotes (
T11951 21854-21858 NNP denotes Fig.
T11952 21859-21860 CD denotes 4
T11953 21861-21862 NNP denotes C
T11954 21862-21863 -RRB- denotes )
T11955 21863-21864 . denotes .
T11956 21865-21870 NN denotes Runx3
T11957 21871-21873 VBZ denotes is
T11958 21874-21881 JJ denotes present
T11959 21882-21884 IN denotes in
T11960 21885-21890 JJ denotes naive
T11961 21891-21894 NNP denotes CD8
T11962 21894-21895 CD denotes +
T11963 21896-21897 NNP denotes T
T11964 21898-21903 NNS denotes cells
T11965 21904-21910 IN denotes before
T11966 21911-21921 NN denotes activation
T11967 21922-21923 -LRB- denotes (
T11968 21923-21925 CD denotes 12
T11969 21925-21926 -RRB- denotes )
T11970 21926-21927 . denotes .
T11971 21928-21930 PRP denotes It
T11972 21931-21940 VBZ denotes represses
T11973 21941-21946 NNP denotes Runx1
T11974 21947-21950 CC denotes and
T11975 21951-21954 VBZ denotes has
T11976 21955-21956 DT denotes a
T11977 21957-21965 JJ denotes positive
T11978 21966-21970 NN denotes role
T11979 21971-21973 IN denotes in
T11980 21974-21977 DT denotes the
T11981 21978-21987 NN denotes induction
T11982 21988-21990 IN denotes of
T11983 21991-21996 NNP denotes Eomes
T11984 21996-21997 , denotes ,
T11985 21998-22006 NN denotes granzyme
T11986 22007-22008 NNP denotes B
T11987 22008-22009 , denotes ,
T11988 22010-22018 NN denotes perforin
T11989 22018-22019 , denotes ,
T11990 22020-22023 CC denotes and
T11991 22024-22029 NNP denotes IFN-γ
T11992 22029-22030 . denotes .
T11993 22031-22036 JJ denotes Runx3
T11994 22037-22042 NNS denotes binds
T11995 22043-22045 TO denotes to
T11996 22046-22055 NNS denotes promoters
T11997 22056-22059 CC denotes and
T11998 22060-22068 JJ denotes putative
T11999 22069-22079 JJ denotes regulatory
T12000 22080-22087 NNS denotes regions
T12001 22088-22090 IN denotes of
T12002 22091-22094 DT denotes the
T12003 22095-22101 JJ denotes latter
T12004 22102-22107 CD denotes three
T12005 22108-22113 NNS denotes genes
T12006 22113-22114 , denotes ,
T12007 22115-22125 VBG denotes suggesting
T12008 22126-22127 DT denotes a
T12009 22128-22134 JJ denotes direct
T12010 22135-22141 NN denotes effect
T12011 22142-22144 IN denotes on
T12012 22145-22149 NN denotes gene
T12013 22150-22160 NN denotes expression
T12014 22160-22161 . denotes .
T12015 22162-22172 JJ denotes Additional
T12016 22173-22184 NNS denotes experiments
T12017 22185-22188 VBP denotes are
T12018 22189-22195 VBN denotes needed
T12019 22196-22198 TO denotes to
T12020 22199-22208 VB denotes determine
T12021 22209-22216 IN denotes whether
T12022 22217-22222 NNP denotes Eomes
T12023 22223-22226 CC denotes and
T12024 22227-22232 NNP denotes Runx1
T12025 22233-22236 VBP denotes are
T12026 22237-22241 RB denotes also
T12027 22242-22248 JJ denotes direct
T12028 22249-22255 NN denotes target
T12029 22256-22261 NNS denotes genes
T12030 22262-22264 IN denotes of
T12031 22265-22270 NNP denotes Runx3
T12032 22270-22271 . denotes .
T12033 22272-22284 RB denotes Surprisingly
T12034 22284-22285 , denotes ,
T12035 22286-22291 JJ denotes Runx3
T12036 22292-22303 VBD denotes contributed
T12037 22304-22306 TO denotes to
T12038 22307-22310 DT denotes the
T12039 22311-22318 JJ denotes optimal
T12040 22319-22329 NN denotes expression
T12041 22330-22332 IN denotes of
T12042 22333-22336 NNP denotes TNF
T12043 22336-22337 , denotes ,
T12044 22338-22342 NNP denotes IL-2
T12045 22342-22343 , denotes ,
T12046 22344-22347 CC denotes and
T12047 22348-22353 NNP denotes IFN-γ
T12048 22354-22356 IN denotes at
T12049 22357-22360 NN denotes day
T12050 22361-22362 CD denotes 4
T12051 22363-22364 -LRB- denotes (
T12052 22364-22367 NN denotes Fig
T12053 22367-22368 . denotes .
T12054 22369-22371 CD denotes S2
T12055 22371-22372 -RRB- denotes )
T12056 22372-22373 . denotes .
T12057 22374-22377 IN denotes For
T12058 22378-22381 NNP denotes TNF
T12059 22382-22385 CC denotes and
T12060 22386-22390 NNP denotes IL-2
T12061 22390-22391 , denotes ,
T12062 22392-22395 DT denotes the
T12063 22396-22407 NN denotes requirement
T12064 22408-22411 IN denotes for
T12065 22412-22417 JJ denotes Runx3
T12066 22418-22426 NNS denotes subsides
T12067 22427-22429 IN denotes by
T12068 22430-22433 NN denotes day
T12069 22434-22435 CD denotes 6
T12070 22436-22437 -LRB- denotes (
T12071 22437-22441 NN denotes Fig.
T12072 22442-22443 CD denotes 3
T12073 22444-22445 NNP denotes C
T12074 22445-22446 -RRB- denotes )
T12075 22446-22447 , denotes ,
T12076 22448-22456 RB denotes possibly
T12077 22457-22464 IN denotes because
T12078 22465-22467 IN denotes of
T12079 22468-22480 NN denotes compensation
T12080 22481-22483 IN denotes by
T12081 22484-22489 NNP denotes Runx1
T12082 22489-22490 , denotes ,
T12083 22491-22496 WDT denotes which
T12084 22497-22499 VBZ denotes is
T12085 22500-22511 VBN denotes derepressed
T12086 22512-22514 IN denotes in
T12087 22515-22520 NNP denotes Runx3
T12088 22520-22521 NNP denotes −
T12089 22521-22522 VBD denotes /
T12090 22522-22523 CD denotes −
T12091 22524-22529 NNS denotes cells
T12092 22530-22531 -LRB- denotes (
T12093 22531-22535 NN denotes Fig.
T12094 22536-22537 CD denotes 4
T12095 22538-22539 NNP denotes A
T12096 22539-22540 -RRB- denotes )
T12097 22540-22541 . denotes .
T12098 22542-22547 JJ denotes Runx3
T12099 22548-22557 VBZ denotes continues
T12100 22558-22560 TO denotes to
T12101 22561-22563 VB denotes be
T12102 22564-22572 VBN denotes required
T12103 22573-22576 IN denotes for
T12104 22577-22582 JJ denotes IFN-γ
T12105 22583-22593 NN denotes expression
T12106 22594-22598 RB denotes even
T12107 22599-22601 IN denotes at
T12108 22602-22605 NN denotes day
T12109 22606-22607 CD denotes 6
T12110 22607-22608 , denotes ,
T12111 22609-22616 RB denotes perhaps
T12112 22617-22624 IN denotes because
T12113 22625-22627 IN denotes of
T12114 22628-22631 PRP$ denotes its
T12115 22632-22636 NN denotes role
T12116 22637-22639 IN denotes in
T12117 22640-22643 DT denotes the
T12118 22644-22653 NN denotes induction
T12119 22654-22656 IN denotes of
T12120 22657-22662 NNP denotes Eomes
T12121 22663-22673 NN denotes expression
T12122 22674-22675 -LRB- denotes (
T12123 22675-22679 JJ denotes Fig.
T12124 22680-22681 CD denotes 4
T12125 22682-22683 NN denotes A
T12126 22683-22684 -RRB- denotes )
T12127 22684-22685 . denotes .
T12128 22686-22688 DT denotes An
T12129 22689-22699 JJ denotes unexpected
T12130 22700-22707 NN denotes finding
T12131 22708-22711 VBD denotes was
T12132 22712-22716 IN denotes that
T12133 22717-22720 DT denotes the
T12134 22721-22724 CD denotes two
T12135 22725-22730 JJ denotes T-box
T12136 22731-22744 NN denotes transcription
T12137 22745-22752 NNS denotes factors
T12138 22752-22753 , denotes ,
T12139 22754-22759 NNP denotes T-bet
T12140 22760-22763 CC denotes and
T12141 22764-22769 NNP denotes Eomes
T12142 22769-22770 , denotes ,
T12143 22771-22774 VBP denotes are
T12144 22775-22787 JJ denotes up-regulated
T12145 22788-22792 IN denotes with
T12146 22793-22797 RB denotes very
T12147 22798-22807 JJ denotes different
T12148 22808-22816 NNS denotes kinetics
T12149 22817-22819 IN denotes in
T12150 22820-22823 CD denotes CD8
T12151 22823-22824 CD denotes +
T12152 22825-22826 NNP denotes T
T12153 22827-22832 NNS denotes cells
T12154 22833-22838 IN denotes under
T12155 22839-22842 PRP$ denotes our
T12156 22843-22850 NN denotes culture
T12157 22851-22861 NNS denotes conditions
T12158 22862-22865 CC denotes and
T12159 22866-22870 VBP denotes have
T12160 22871-22883 JJ denotes nonredundant
T12161 22884-22889 NNS denotes roles
T12162 22890-22892 IN denotes in
T12163 22893-22896 DT denotes the
T12164 22897-22907 JJ denotes subsequent
T12165 22908-22918 NN denotes expression
T12166 22919-22921 IN denotes of
T12167 22922-22925 JJ denotes key
T12168 22926-22934 NN denotes effector
T12169 22935-22943 NNS denotes proteins
T12170 22944-22945 -LRB- denotes (
T12171 22945-22949 NN denotes Fig.
T12172 22950-22951 CD denotes 4
T12173 22952-22953 NNP denotes C
T12174 22953-22954 -RRB- denotes )
T12175 22954-22955 . denotes .
T12176 22956-22961 NN denotes T-bet
T12177 22962-22964 VBZ denotes is
T12178 22965-22971 VBN denotes needed
T12179 22972-22977 RB denotes early
T12180 22978-22980 TO denotes to
T12181 22981-22987 VB denotes confer
T12182 22988-22990 IN denotes on
T12183 22991-23000 VBN denotes activated
T12184 23001-23004 NNP denotes CD8
T12185 23004-23005 CD denotes +
T12186 23006-23007 NNP denotes T
T12187 23008-23013 NNS denotes cells
T12188 23014-23017 DT denotes the
T12189 23018-23028 NN denotes competence
T12190 23029-23031 TO denotes to
T12191 23032-23039 VB denotes produce
T12192 23040-23045 JJ denotes IFN-γ
T12193 23046-23050 IN denotes upon
T12194 23051-23064 NN denotes restimulation
T12195 23064-23065 , denotes ,
T12196 23066-23069 CC denotes but
T12197 23070-23073 PRP$ denotes its
T12198 23074-23082 NN denotes function
T12199 23083-23085 VBZ denotes is
T12200 23086-23090 RBR denotes less
T12201 23091-23100 JJ denotes important
T12202 23101-23103 IN denotes at
T12203 23104-23109 JJ denotes later
T12204 23110-23115 NNS denotes times
T12205 23115-23116 . denotes .
T12206 23117-23122 NNS denotes Eomes
T12207 23122-23123 , denotes ,
T12208 23124-23129 WDT denotes which
T12209 23130-23132 VBZ denotes is
T12210 23133-23140 VBN denotes induced
T12211 23141-23145 RB denotes late
T12212 23146-23149 CC denotes and
T12213 23150-23159 NNS denotes functions
T12214 23160-23170 JJ denotes downstream
T12215 23171-23173 IN denotes of
T12216 23174-23179 NNP denotes Runx3
T12217 23179-23180 , denotes ,
T12218 23181-23184 MD denotes may
T12219 23185-23195 VB denotes substitute
T12220 23196-23199 IN denotes for
T12221 23200-23205 NN denotes T-bet
T12222 23206-23208 IN denotes in
T12223 23209-23218 VBG denotes promoting
T12224 23219-23222 DT denotes the
T12225 23223-23228 JJ denotes acute
T12226 23229-23239 NN denotes expression
T12227 23240-23242 IN denotes of
T12228 23243-23248 NNP denotes IFN-γ
T12229 23249-23251 IN denotes in
T12230 23252-23264 JJ denotes restimulated
T12231 23265-23269 NNS denotes CTLs
T12232 23270-23271 -LRB- denotes (
T12233 23271-23272 CD denotes 8
T12234 23272-23273 -RRB- denotes )
T12235 23273-23274 . denotes .
T12236 23275-23281 RB denotes Indeed
T12237 23281-23282 , denotes ,
T12238 23283-23288 NNP denotes T-bet
T12239 23289-23292 CC denotes and
T12240 23293-23298 NNP denotes Eomes
T12241 23299-23303 DT denotes both
T12242 23304-23314 VBP denotes contribute
T12243 23315-23317 TO denotes to
T12244 23318-23326 VB denotes perforin
T12245 23327-23337 NN denotes expression
T12246 23338-23340 IN denotes in
T12247 23341-23343 NNP denotes NK
T12248 23344-23349 NNS denotes cells
T12249 23350-23351 -LRB- denotes (
T12250 23351-23352 CD denotes 8
T12251 23352-23353 , denotes ,
T12252 23354-23356 CD denotes 26
T12253 23356-23357 -RRB- denotes )
T12254 23357-23358 , denotes ,
T12255 23359-23362 CC denotes and
T12256 23363-23368 NNP denotes Eomes
T12257 23369-23376 VBZ denotes induces
T12258 23377-23385 NN denotes granzyme
T12259 23386-23387 NN denotes B
T12260 23388-23390 IN denotes as
T12261 23391-23402 RB denotes effectively
T12262 23403-23405 IN denotes as
T12263 23406-23411 NN denotes T-bet
T12264 23412-23414 IN denotes in
T12265 23415-23425 VBG denotes developing
T12266 23426-23429 JJ denotes Th2
T12267 23430-23435 NNS denotes cells
T12268 23436-23437 -LRB- denotes (
T12269 23437-23438 CD denotes 7
T12270 23438-23439 -RRB- denotes )
T12271 23439-23440 : denotes ;
T12272 23441-23445 RB denotes thus
T12273 23445-23446 , denotes ,
T12274 23447-23450 DT denotes the
T12275 23451-23459 JJ denotes relative
T12276 23460-23465 NNS denotes roles
T12277 23466-23468 IN denotes of
T12278 23469-23474 DT denotes these
T12279 23475-23480 JJ denotes T-box
T12280 23481-23494 NN denotes transcription
T12281 23495-23502 NNS denotes factors
T12282 23503-23507 VBP denotes vary
T12283 23508-23517 VBG denotes depending
T12284 23518-23520 IN denotes on
T12285 23521-23525 NN denotes cell
T12286 23526-23530 NN denotes type
T12287 23530-23531 . denotes .
T12288 23532-23544 RB denotes Surprisingly
T12289 23544-23545 , denotes ,
T12290 23546-23553 RB denotes however
T12291 23553-23554 , denotes ,
T12292 23555-23560 NNP denotes Eomes
T12293 23561-23564 CC denotes and
T12294 23565-23570 NNP denotes T-bet
T12295 23571-23579 VBD denotes appeared
T12296 23580-23592 JJ denotes nonredundant
T12297 23593-23595 IN denotes in
T12298 23596-23601 PRP$ denotes their
T12299 23602-23609 NN denotes ability
T12300 23610-23612 TO denotes to
T12301 23613-23619 VB denotes induce
T12302 23620-23623 CD denotes two
T12303 23624-23629 JJ denotes other
T12304 23630-23637 NNS denotes markers
T12305 23638-23640 IN denotes of
T12306 23641-23644 NNP denotes CTL
T12307 23645-23653 NN denotes function
T12308 23653-23654 , denotes ,
T12309 23655-23659 NNP denotes Prf1
T12310 23660-23663 CC denotes and
T12311 23664-23668 NNP denotes Gzmb
T12312 23669-23670 -LRB- denotes (
T12313 23670-23674 NNP denotes Fig.
T12314 23675-23676 CD denotes 4
T12315 23677-23678 NNP denotes C
T12316 23678-23679 -RRB- denotes )
T12317 23679-23680 . denotes .
T12318 23681-23687 RB denotes Rather
T12319 23687-23688 , denotes ,
T12320 23689-23694 NN denotes T-bet
T12321 23695-23698 CC denotes and
T12322 23699-23704 NNS denotes Eomes
T12323 23705-23709 VBD denotes were
T12324 23710-23718 VBN denotes involved
T12325 23719-23721 IN denotes in
T12326 23722-23732 VBG denotes regulating
T12327 23733-23741 NN denotes granzyme
T12328 23742-23743 NN denotes B
T12329 23744-23747 CC denotes and
T12330 23748-23756 NN denotes perforin
T12331 23757-23767 NN denotes expression
T12332 23767-23768 , denotes ,
T12333 23769-23781 RB denotes respectively
T12334 23781-23782 : denotes :
T12335 23783-23796 NN denotes up-regulation
T12336 23797-23799 IN denotes of
T12337 23800-23805 NNP denotes T-bet
T12338 23806-23809 CC denotes and
T12339 23810-23815 NNP denotes Eomes
T12340 23816-23820 NNP denotes mRNA
T12341 23821-23824 CC denotes and
T12342 23825-23832 NN denotes protein
T12343 23833-23840 RB denotes closely
T12344 23841-23849 VBD denotes preceded
T12345 23850-23863 NN denotes up-regulation
T12346 23864-23866 IN denotes of
T12347 23867-23871 NNP denotes Gzmb
T12348 23872-23875 CC denotes and
T12349 23876-23880 NNP denotes Prf1
T12350 23881-23885 NNP denotes mRNA
T12351 23886-23889 CC denotes and
T12352 23890-23897 NN denotes protein
T12353 23897-23898 , denotes ,
T12354 23899-23911 RB denotes respectively
T12355 23911-23912 . denotes .
T12356 23913-23918 NN denotes T-bet
T12357 23919-23922 VBD denotes had
T12358 23923-23925 DT denotes no
T12359 23926-23930 NN denotes role
T12360 23931-23933 IN denotes in
T12361 23934-23942 NN denotes perforin
T12362 23943-23953 NN denotes expression
T12363 23954-23959 IN denotes under
T12364 23960-23963 PRP$ denotes our
T12365 23964-23971 NN denotes culture
T12366 23972-23982 NNS denotes conditions
T12367 23982-23983 , denotes ,
T12368 23984-23987 CC denotes and
T12369 23988-23995 NNP denotes Eo-VP16
T12370 23996-23999 VBD denotes did
T12371 24000-24003 RB denotes not
T12372 24004-24010 VB denotes affect
T12373 24011-24019 NN denotes granzyme
T12374 24020-24021 NNP denotes B
T12375 24022-24032 NN denotes expression
T12376 24033-24037 WRB denotes when
T12377 24038-24047 VBN denotes expressed
T12378 24048-24050 IN denotes in
T12379 24051-24056 NNP denotes T-bet
T12380 24056-24057 CD denotes −
T12381 24057-24058 CD denotes /
T12382 24058-24059 NN denotes −
T12383 24060-24062 CC denotes or
T12384 24063-24068 JJ denotes Runx3
T12385 24068-24069 NN denotes −
T12386 24069-24070 NN denotes /
T12387 24070-24071 NN denotes −
T12388 24072-24077 NNS denotes cells
T12389 24077-24078 . denotes .
T12390 24079-24086 IN denotes Because
T12391 24087-24099 JJ denotes conventional
T12392 24100-24115 JJ denotes Eomes-deficient
T12393 24116-24120 NNS denotes mice
T12394 24121-24124 VBP denotes die
T12395 24125-24131 IN denotes before
T12396 24132-24141 NN denotes precursor
T12397 24142-24147 NNS denotes cells
T12398 24148-24151 MD denotes can
T12399 24152-24154 VB denotes be
T12400 24155-24163 VBN denotes isolated
T12401 24164-24167 IN denotes for
T12402 24168-24172 NN denotes bone
T12403 24173-24179 NN denotes marrow
T12404 24180-24189 NNS denotes transfers
T12405 24190-24191 -LRB- denotes (
T12406 24191-24193 CD denotes 27
T12407 24193-24194 -RRB- denotes )
T12408 24195-24198 CC denotes and
T12409 24199-24206 IN denotes because
T12410 24207-24208 NNP denotes T
T12411 24209-24214 NNS denotes cells
T12412 24215-24228 RB denotes conditionally
T12413 24229-24238 VBP denotes deficient
T12414 24239-24241 IN denotes in
T12415 24242-24247 NNS denotes Eomes
T12416 24248-24252 VBP denotes have
T12417 24253-24257 RB denotes only
T12418 24258-24266 RB denotes recently
T12419 24267-24271 VBN denotes been
T12420 24272-24281 VBN denotes described
T12421 24282-24283 -LRB- denotes (
T12422 24283-24284 CD denotes 9
T12423 24284-24285 -RRB- denotes )
T12424 24285-24286 , denotes ,
T12425 24287-24289 PRP denotes we
T12426 24290-24294 VBD denotes were
T12427 24295-24301 JJ denotes unable
T12428 24302-24304 TO denotes to
T12429 24305-24314 VB denotes introduce
T12430 24315-24320 JJ denotes Runx3
T12431 24321-24325 IN denotes into
T12432 24326-24341 JJ denotes Eomes-deficient
T12433 24342-24345 NNP denotes CD8
T12434 24345-24346 CD denotes +
T12435 24347-24348 NNP denotes T
T12436 24349-24354 NNS denotes cells
T12437 24355-24357 TO denotes to
T12438 24358-24362 VB denotes test
T12439 24363-24371 RB denotes formally
T12440 24372-24379 IN denotes whether
T12441 24380-24385 NNP denotes Eomes
T12442 24386-24396 VBD denotes cooperated
T12443 24397-24401 IN denotes with
T12444 24402-24407 JJ denotes Runx3
T12445 24408-24410 TO denotes to
T12446 24411-24417 VB denotes induce
T12447 24418-24426 JJ denotes perforin
T12448 24427-24437 NN denotes expression
T12449 24437-24438 . denotes .
T12450 24439-24451 RB denotes Collectively
T12451 24451-24452 , denotes ,
T12452 24453-24456 PRP$ denotes our
T12453 24457-24461 NNS denotes data
T12454 24462-24465 VBP denotes are
T12455 24466-24476 JJ denotes consistent
T12456 24477-24481 IN denotes with
T12457 24482-24483 DT denotes a
T12458 24484-24499 JJ denotes transcriptional
T12459 24500-24507 NN denotes network
T12460 24508-24510 IN denotes in
T12461 24511-24516 WDT denotes which
T12462 24517-24528 VBG denotes preexisting
T12463 24529-24534 JJ denotes Runx3
T12464 24535-24545 NNS denotes cooperates
T12465 24546-24550 IN denotes with
T12466 24551-24554 DT denotes the
T12467 24555-24562 JJ denotes induced
T12468 24563-24568 NN denotes T-box
T12469 24569-24576 NNS denotes factors
T12470 24577-24582 NNP denotes T-bet
T12471 24583-24586 CC denotes and
T12472 24587-24592 NNP denotes Eomes
T12473 24593-24596 CC denotes and
T12474 24597-24603 NNP denotes IL-2Rβ
T12475 24604-24611 NNS denotes signals
T12476 24612-24613 -LRB- denotes (
T12477 24613-24624 JJ denotes unpublished
T12478 24625-24629 NNS denotes data
T12479 24629-24630 -RRB- denotes )
T12480 24631-24633 TO denotes to
T12481 24634-24645 VB denotes orchestrate
T12482 24646-24649 NNP denotes CTL
T12483 24650-24665 NN denotes differentiation
T12484 24666-24667 -LRB- denotes (
T12485 24667-24671 JJ denotes Fig.
T12486 24672-24673 CD denotes 4
T12487 24674-24675 NNP denotes C
T12488 24675-24676 -RRB- denotes )
T12489 24676-24677 . denotes .
T12490 24678-24681 PRP$ denotes Our
T12491 24682-24686 NNS denotes data
T12492 24687-24693 VBP denotes recall
T12493 24694-24697 DT denotes the
T12494 24699-24711 JJ denotes feed-forward
T12495 24713-24724 NN denotes interaction
T12496 24725-24732 IN denotes between
T12497 24733-24738 NNP denotes T-bet
T12498 24739-24742 CC denotes and
T12499 24743-24748 NNP denotes Runx3
T12500 24749-24753 IN denotes that
T12501 24754-24756 PRP denotes we
T12502 24757-24767 RB denotes previously
T12503 24768-24777 VBD denotes described
T12504 24778-24780 IN denotes in
T12505 24781-24784 NNP denotes CD4
T12506 24784-24785 NNP denotes +
T12507 24786-24787 -LRB- denotes (
T12508 24787-24790 NNP denotes Th1
T12509 24790-24791 -RRB- denotes )
T12510 24792-24793 NNP denotes T
T12511 24794-24799 NNS denotes cells
T12512 24800-24801 -LRB- denotes (
T12513 24801-24803 CD denotes 15
T12514 24803-24804 -RRB- denotes )
T12515 24805-24808 CC denotes but
T12516 24809-24812 VBP denotes are
T12517 24813-24821 JJ denotes distinct
T12518 24822-24824 IN denotes in
T12519 24825-24828 CD denotes two
T12520 24829-24837 NNS denotes respects
T12521 24837-24838 : denotes :
T12522 24839-24841 IN denotes in
T12523 24842-24857 VBG denotes differentiating
T12524 24858-24861 JJ denotes Th1
T12525 24862-24867 NNS denotes cells
T12526 24867-24868 , denotes ,
T12527 24869-24874 NN denotes T-bet
T12528 24875-24877 VBZ denotes is
T12529 24878-24885 VBN denotes induced
T12530 24886-24888 IN denotes by
T12531 24889-24892 NNP denotes TCR
T12532 24893-24900 NNS denotes signals
T12533 24901-24904 CC denotes and
T12534 24905-24910 NNP denotes IFN-γ
T12535 24910-24911 , denotes ,
T12536 24912-24915 CC denotes and
T12537 24916-24918 IN denotes in
T12538 24919-24923 NN denotes turn
T12539 24924-24931 VBZ denotes induces
T12540 24932-24937 NNP denotes Runx3
T12541 24938-24939 -LRB- denotes (
T12542 24939-24941 CD denotes 15
T12543 24941-24942 -RRB- denotes )
T12544 24942-24943 , denotes ,
T12545 24944-24951 IN denotes whereas
T12546 24952-24954 IN denotes in
T12547 24955-24970 VBG denotes differentiating
T12548 24971-24974 NNP denotes CD8
T12549 24974-24975 CD denotes +
T12550 24976-24977 NNP denotes T
T12551 24978-24983 NNS denotes cells
T12552 24983-24984 , denotes ,
T12553 24985-24996 VBG denotes preexisting
T12554 24997-25002 NNP denotes Runx3
T12555 25003-25005 VBZ denotes is
T12556 25006-25014 VBN denotes required
T12557 25015-25017 TO denotes to
T12558 25018-25024 VB denotes induce
T12559 25025-25028 DT denotes the
T12560 25029-25034 NN denotes T-box
T12561 25035-25048 NN denotes transcription
T12562 25049-25055 NN denotes factor
T12563 25056-25061 NNP denotes Eomes
T12564 25061-25062 . denotes .
T12565 25063-25075 JJ denotes Whole-genome
T12566 25076-25087 NNS denotes experiments
T12567 25088-25090 IN denotes in
T12568 25091-25096 DT denotes these
T12569 25097-25100 CC denotes and
T12570 25101-25106 JJ denotes other
T12571 25107-25114 NNS denotes systems
T12572 25115-25119 MD denotes will
T12573 25120-25122 VB denotes be
T12574 25123-25131 VBN denotes required
T12575 25132-25134 TO denotes to
T12576 25135-25144 VB denotes establish
T12577 25145-25152 IN denotes whether
T12578 25153-25164 NN denotes cooperation
T12579 25165-25172 IN denotes between
T12580 25173-25178 NNP denotes T-box
T12581 25179-25182 CC denotes and
T12582 25183-25187 NNP denotes Runx
T12583 25188-25194 NN denotes family
T12584 25195-25208 NN denotes transcription
T12585 25209-25216 NNS denotes factors
T12586 25217-25219 VBZ denotes is
T12587 25220-25221 DT denotes a
T12588 25222-25229 JJ denotes general
T12589 25230-25237 NN denotes feature
T12590 25238-25240 IN denotes of
T12591 25241-25249 JJ denotes cellular
T12592 25250-25265 NN denotes differentiation
T12593 25266-25274 NNS denotes programs
T12594 25274-25275 . denotes .
T13830 25300-25310 NNS denotes Antibodies
T13831 25311-25314 CC denotes and
T13832 25315-25323 NNS denotes reagents
T13833 25323-25324 . denotes .
T13834 25325-25328 DT denotes The
T13835 25329-25338 VBG denotes following
T13836 25339-25349 NNS denotes antibodies
T13837 25350-25354 VBN denotes used
T13838 25355-25358 IN denotes for
T13839 25359-25372 JJ denotes intracellular
T13840 25373-25375 CC denotes or
T13841 25376-25383 NN denotes surface
T13842 25384-25390 NNS denotes stains
T13843 25391-25395 VBD denotes were
T13844 25396-25404 VBN denotes obtained
T13845 25405-25409 IN denotes from
T13846 25410-25421 NNP denotes eBioscience
T13847 25421-25422 : denotes :
T13848 25423-25427 NNS denotes anti
T13849 25428-25432 NN denotes IL-2
T13850 25432-25433 , denotes ,
T13851 25434-25438 NNS denotes anti
T13852 25439-25444 JJ denotes IFN-γ
T13853 25444-25445 , denotes ,
T13854 25446-25454 JJ denotes anti-TNF
T13855 25454-25455 , denotes ,
T13856 25456-25460 NNS denotes anti
T13857 25461-25469 NN denotes granzyme
T13858 25470-25471 NN denotes B
T13859 25471-25472 , denotes ,
T13860 25473-25481 JJ denotes anti-CD8
T13861 25481-25482 , denotes ,
T13862 25483-25492 JJ denotes anti-CD25
T13863 25492-25493 , denotes ,
T13864 25494-25497 CC denotes and
T13865 25498-25507 JJ denotes anti-CD44
T13866 25507-25508 . denotes .
T13867 25509-25518 NN denotes Anti-CD69
T13868 25519-25522 VBD denotes was
T13869 25523-25532 VBN denotes purchased
T13870 25533-25537 IN denotes from
T13871 25538-25540 NNP denotes BD
T13872 25540-25541 . denotes .
T13873 25542-25545 IN denotes For
T13874 25546-25550 NNP denotes ChIP
T13875 25551-25562 NNS denotes experiments
T13876 25562-25563 , denotes ,
T13877 25564-25567 DT denotes the
T13878 25568-25578 JJ denotes anti-Eomes
T13879 25579-25587 NN denotes antibody
T13880 25588-25591 VBD denotes was
T13881 25592-25600 VBN denotes obtained
T13882 25601-25605 IN denotes from
T13883 25606-25611 NNP denotes Abcam
T13884 25612-25615 CC denotes and
T13885 25616-25619 DT denotes the
T13886 25620-25630 JJ denotes anti-Runx3
T13887 25631-25639 NN denotes antibody
T13888 25640-25643 VBD denotes was
T13889 25644-25652 VBN denotes produced
T13890 25653-25655 IN denotes by
T13891 25656-25659 DT denotes the
T13892 25660-25666 NNP denotes Groner
T13893 25667-25677 NN denotes laboratory
T13894 25677-25678 . denotes .
T13895 25679-25682 DT denotes The
T13896 25683-25692 VBG denotes following
T13897 25693-25703 NNS denotes antibodies
T13898 25704-25708 VBD denotes were
T13899 25709-25713 VBN denotes used
T13900 25714-25717 IN denotes for
T13901 25718-25732 VBG denotes immunoblotting
T13902 25732-25733 : denotes :
T13903 25734-25746 NN denotes antiperforin
T13904 25747-25748 -LRB- denotes (
T13905 25748-25753 NNP denotes Abcam
T13906 25753-25754 -RRB- denotes )
T13907 25754-25755 , denotes ,
T13908 25756-25766 JJ denotes anti-Eomes
T13909 25767-25768 -LRB- denotes (
T13910 25768-25773 NNP denotes Abcam
T13911 25773-25774 -RRB- denotes )
T13912 25774-25775 , denotes ,
T13913 25776-25779 CC denotes and
T13914 25780-25784 NNS denotes anti
T13915 25785-25791 NNP denotes Pol-II
T13916 25792-25793 -LRB- denotes (
T13917 25793-25798 NNP denotes Santa
T13918 25799-25803 NNP denotes Cruz
T13919 25804-25817 NNP denotes Biotechnology
T13920 25817-25818 , denotes ,
T13921 25819-25823 NNP denotes Inc.
T13922 25823-25824 -RRB- denotes )
T13923 25824-25825 . denotes .
T13924 25826-25829 DT denotes The
T13925 25830-25835 NN denotes T-bet
T13926 25836-25844 NN denotes antibody
T13927 25845-25848 VBD denotes was
T13928 25849-25857 VBN denotes provided
T13929 25858-25860 IN denotes by
T13930 25861-25863 NNP denotes L.
T13931 25864-25872 NNP denotes Glimcher
T13932 25873-25874 -LRB- denotes (
T13933 25874-25881 NNP denotes Harvard
T13934 25882-25888 NNP denotes School
T13935 25889-25891 IN denotes of
T13936 25892-25898 NNP denotes Public
T13937 25899-25905 NNP denotes Health
T13938 25905-25906 , denotes ,
T13939 25907-25913 NNP denotes Boston
T13940 25913-25914 , denotes ,
T13941 25915-25917 NNP denotes MA
T13942 25917-25918 -RRB- denotes )
T13943 25918-25919 . denotes .
T13944 25920-25923 DT denotes The
T13945 25924-25933 VBG denotes following
T13946 25934-25942 NNS denotes reagents
T13947 25943-25947 VBD denotes were
T13948 25948-25952 VBN denotes used
T13949 25953-25956 IN denotes for
T13950 25957-25960 DT denotes the
T13951 25961-25972 NNS denotes experiments
T13952 25973-25982 VBN denotes presented
T13953 25983-25985 IN denotes in
T13954 25986-25990 DT denotes this
T13955 25991-25997 NN denotes report
T13956 25997-25998 : denotes :
T13957 25999-26006 NNP denotes Annexin
T13958 26007-26008 NNP denotes V
T13959 26009-26013 NNP denotes FITC
T13960 26014-26023 NNP denotes Apoptosis
T13961 26024-26033 NNP denotes Detection
T13962 26034-26037 NNP denotes Kit
T13963 26038-26039 -LRB- denotes (
T13964 26039-26041 NNP denotes BD
T13965 26041-26042 -RRB- denotes )
T13966 26042-26043 , denotes ,
T13967 26044-26047 NNP denotes CD8
T13968 26048-26056 JJ denotes Negative
T13969 26057-26066 NNP denotes Isolation
T13970 26067-26070 NNP denotes Kit
T13971 26071-26072 -LRB- denotes (
T13972 26072-26082 NNP denotes Invitrogen
T13973 26082-26083 -RRB- denotes )
T13974 26083-26084 , denotes ,
T13975 26085-26088 NNP denotes CD8
T13976 26089-26099 NNP denotes MicroBeads
T13977 26100-26101 -LRB- denotes (
T13978 26101-26109 NNP denotes Miltenyi
T13979 26110-26116 NNP denotes Biotec
T13980 26116-26117 -RRB- denotes )
T13981 26117-26118 , denotes ,
T13982 26119-26122 CC denotes and
T13983 26123-26127 NNP denotes SYBR
T13984 26128-26133 NNP denotes Green
T13985 26134-26137 NNP denotes PCR
T13986 26138-26142 NNP denotes Core
T13987 26143-26151 NNP denotes Reagents
T13988 26152-26153 -LRB- denotes (
T13989 26153-26160 NNP denotes Applied
T13990 26161-26171 NNPS denotes Biosystems
T13991 26171-26172 -RRB- denotes )
T13992 26172-26173 . denotes .
T13993 26174-26177 DT denotes The
T13994 26178-26182 JJ denotes Gp33
T13995 26183-26190 NN denotes peptide
T13996 26191-26192 -LRB- denotes (
T13997 26192-26201 NNP denotes KAVYNFATC
T13998 26201-26202 -RRB- denotes )
T13999 26203-26206 VBD denotes was
T14000 26207-26218 VBN denotes synthesized
T14001 26219-26221 IN denotes by
T14002 26222-26225 DT denotes the
T14003 26226-26231 NNP denotes Tufts
T14004 26232-26242 NNP denotes University
T14005 26243-26247 NNP denotes Core
T14006 26248-26256 NNP denotes Facility
T14007 26256-26257 , denotes ,
T14008 26258-26261 CC denotes and
T14009 26262-26264 CD denotes 10
T14010 26265-26267 NN denotes mM
T14011 26268-26270 IN denotes of
T14012 26271-26276 NN denotes stock
T14013 26277-26286 NNS denotes solutions
T14014 26287-26290 VBD denotes was
T14015 26291-26299 VBN denotes prepared
T14016 26300-26302 IN denotes in
T14017 26303-26307 NNP denotes DMSO
T14018 26307-26308 . denotes .
T14382 26310-26319 NN denotes Isolation
T14383 26320-26323 CC denotes and
T14384 26324-26331 NN denotes culture
T14385 26332-26334 IN denotes of
T14386 26335-26342 JJ denotes primary
T14387 26343-26346 NNP denotes CD8
T14388 26346-26347 CD denotes +
T14389 26348-26349 NNP denotes T
T14390 26350-26355 NNS denotes cells
T14391 26355-26356 . denotes .
T14392 26357-26360 CD denotes CD8
T14393 26360-26361 CD denotes +
T14394 26362-26363 NNP denotes T
T14395 26364-26369 NNS denotes cells
T14396 26370-26374 IN denotes from
T14397 26375-26376 CD denotes 4
T14398 26377-26385 JJ denotes 8-wk-old
T14399 26386-26390 NNP denotes Tcra
T14400 26390-26391 FW denotes −
T14401 26391-26392 FW denotes /
T14402 26392-26393 FW denotes −
T14403 26394-26395 FW denotes ×
T14404 26396-26399 FW denotes P14
T14405 26400-26403 FW denotes TCR
T14406 26404-26414 FW denotes transgenic
T14407 26415-26416 -LRB- denotes (
T14408 26416-26423 NNP denotes Taconic
T14409 26423-26424 -RRB- denotes )
T14410 26424-26425 , denotes ,
T14411 26426-26434 NNP denotes C57BL/6J
T14412 26435-26437 NNP denotes WT
T14413 26437-26438 , denotes ,
T14414 26439-26441 CC denotes or
T14415 26442-26447 CD denotes Tbx21
T14416 26447-26448 CD denotes −
T14417 26448-26449 NN denotes /
T14418 26449-26450 NN denotes −
T14419 26451-26452 -LRB- denotes (
T14420 26452-26455 DT denotes The
T14421 26456-26463 NNP denotes Jackson
T14422 26464-26474 NNP denotes Laboratory
T14423 26474-26475 -RRB- denotes )
T14424 26476-26480 NNS denotes mice
T14425 26481-26485 VBD denotes were
T14426 26486-26494 VBN denotes purified
T14427 26495-26496 -LRB- denotes (
T14428 26496-26497 FW denotes >
T14429 26497-26499 CD denotes 95
T14430 26499-26500 NN denotes %
T14431 26501-26507 NN denotes purity
T14432 26507-26508 -RRB- denotes )
T14433 26509-26511 IN denotes by
T14434 26512-26520 JJ denotes negative
T14435 26521-26530 NN denotes selection
T14436 26531-26532 -LRB- denotes (
T14437 26532-26542 NNP denotes Invitrogen
T14438 26542-26543 -RRB- denotes )
T14439 26544-26548 IN denotes from
T14440 26549-26555 VBN denotes pooled
T14441 26556-26562 NN denotes spleen
T14442 26563-26566 CC denotes and
T14443 26567-26572 NN denotes lymph
T14444 26573-26577 NN denotes node
T14445 26578-26583 NNS denotes cells
T14446 26583-26584 . denotes .
T14447 26585-26588 CD denotes CD8
T14448 26588-26589 CD denotes +
T14449 26590-26591 NNP denotes T
T14450 26592-26597 NNS denotes cells
T14451 26598-26602 IN denotes from
T14452 26603-26608 JJ denotes Runx3
T14453 26608-26609 NN denotes −
T14454 26609-26610 NN denotes /
T14455 26610-26611 NN denotes −
T14456 26612-26616 NNS denotes mice
T14457 26617-26619 IN denotes on
T14458 26620-26623 DT denotes the
T14459 26624-26627 NNP denotes ICR
T14460 26628-26638 NN denotes background
T14461 26639-26643 VBD denotes were
T14462 26644-26652 VBN denotes purified
T14463 26653-26655 IN denotes by
T14464 26656-26664 JJ denotes positive
T14465 26665-26674 NN denotes selection
T14466 26675-26676 -LRB- denotes (
T14467 26676-26684 NNP denotes Miltenyi
T14468 26685-26691 NNP denotes Biotec
T14469 26691-26692 -RRB- denotes )
T14470 26692-26693 . denotes .
T14471 26694-26697 DT denotes All
T14472 26698-26702 NNS denotes mice
T14473 26703-26707 VBD denotes were
T14474 26708-26718 VBN denotes maintained
T14475 26719-26721 IN denotes in
T14476 26722-26730 JJ denotes specific
T14477 26731-26744 JJ denotes pathogen-free
T14478 26745-26752 NN denotes barrier
T14479 26753-26763 NNS denotes facilities
T14480 26764-26767 CC denotes and
T14481 26768-26772 VBN denotes used
T14482 26773-26782 VBG denotes according
T14483 26783-26785 TO denotes to
T14484 26786-26795 NNS denotes protocols
T14485 26796-26804 VBN denotes approved
T14486 26805-26807 IN denotes by
T14487 26808-26811 DT denotes the
T14488 26812-26818 NNP denotes Immune
T14489 26819-26826 NNP denotes Disease
T14490 26827-26836 NNP denotes Institute
T14491 26837-26840 CC denotes and
T14492 26841-26844 DT denotes the
T14493 26845-26852 NNP denotes Harvard
T14494 26853-26860 NNP denotes Medical
T14495 26861-26867 NNP denotes School
T14496 26868-26874 NNP denotes Animal
T14497 26875-26879 NNP denotes Care
T14498 26880-26883 CC denotes and
T14499 26884-26887 NNP denotes Use
T14500 26888-26898 NNS denotes Committees
T14501 26898-26899 . denotes .
T14502 26900-26903 IN denotes For
T14503 26904-26915 NN denotes stimulation
T14504 26915-26916 , denotes ,
T14505 26917-26925 VBN denotes purified
T14506 26926-26929 NNP denotes CD8
T14507 26929-26930 CD denotes +
T14508 26931-26932 NNP denotes T
T14509 26933-26938 NNS denotes cells
T14510 26939-26943 VBD denotes were
T14511 26944-26952 VBN denotes cultured
T14512 26953-26955 IN denotes at
T14513 26956-26959 CD denotes 106
T14514 26960-26968 NN denotes cells/ml
T14515 26969-26970 -LRB- denotes (
T14516 26970-26972 CD denotes 10
T14517 26973-26975 NN denotes ml
T14518 26975-26976 -RRB- denotes )
T14519 26977-26979 IN denotes in
T14520 26980-26983 CD denotes T25
T14521 26984-26990 NNS denotes flasks
T14522 26991-26997 VBN denotes coated
T14523 26998-27002 IN denotes with
T14524 27003-27004 CD denotes 1
T14525 27005-27007 NN denotes μg
T14526 27007-27008 NN denotes /
T14527 27008-27010 NN denotes ml
T14528 27011-27015 DT denotes each
T14529 27016-27018 IN denotes of
T14530 27019-27027 JJ denotes anti-CD3
T14531 27028-27029 -LRB- denotes (
T14532 27029-27034 JJ denotes clone
T14533 27035-27039 NNP denotes 2C11
T14534 27039-27040 -RRB- denotes )
T14535 27041-27044 CC denotes and
T14536 27045-27054 JJ denotes anti-CD28
T14537 27055-27056 -LRB- denotes (
T14538 27056-27061 JJ denotes clone
T14539 27062-27067 CD denotes 37.51
T14540 27067-27068 -RRB- denotes )
T14541 27069-27071 IN denotes by
T14542 27072-27084 NN denotes pretreatment
T14543 27085-27089 IN denotes with
T14544 27090-27093 CD denotes 300
T14545 27094-27096 NN denotes μg
T14546 27096-27097 NN denotes /
T14547 27097-27099 NN denotes ml
T14548 27100-27104 NN denotes goat
T14549 27105-27109 NNS denotes anti
T14550 27110-27117 NN denotes hamster
T14551 27118-27121 NNP denotes IgG
T14552 27121-27122 . denotes .
T14553 27123-27128 IN denotes After
T14554 27129-27131 CD denotes 48
T14555 27132-27133 NN denotes h
T14556 27133-27134 , denotes ,
T14557 27135-27140 NNS denotes cells
T14558 27141-27145 VBD denotes were
T14559 27146-27153 VBN denotes removed
T14560 27154-27158 IN denotes from
T14561 27159-27162 DT denotes the
T14562 27163-27166 NNP denotes TCR
T14563 27167-27178 NN denotes stimulation
T14564 27179-27182 CC denotes and
T14565 27183-27193 VBD denotes recultured
T14566 27194-27196 IN denotes at
T14567 27197-27198 DT denotes a
T14568 27199-27212 NN denotes concentration
T14569 27213-27215 IN denotes of
T14570 27216-27217 CD denotes 5
T14571 27218-27219 CD denotes ×
T14572 27220-27223 CD denotes 105
T14573 27224-27232 NN denotes cells/ml
T14574 27233-27235 IN denotes in
T14575 27236-27241 NNS denotes media
T14576 27242-27254 VBN denotes supplemented
T14577 27255-27259 IN denotes with
T14578 27260-27263 CD denotes 100
T14579 27264-27268 NNP denotes U/ml
T14580 27269-27275 NNP denotes rhIL-2
T14581 27275-27276 . denotes .
T14582 27277-27282 DT denotes Every
T14583 27283-27285 CD denotes 24
T14584 27286-27287 NN denotes h
T14585 27287-27288 , denotes ,
T14586 27289-27295 JJ denotes viable
T14587 27296-27301 NNS denotes cells
T14588 27302-27306 VBD denotes were
T14589 27307-27314 VBN denotes counted
T14590 27315-27318 CC denotes and
T14591 27319-27329 VBN denotes readjusted
T14592 27330-27332 TO denotes to
T14593 27333-27334 CD denotes 5
T14594 27335-27336 CD denotes ×
T14595 27337-27340 CD denotes 105
T14596 27341-27349 NN denotes cells/ml
T14597 27350-27354 IN denotes with
T14598 27355-27360 JJ denotes fresh
T14599 27361-27366 NNS denotes media
T14600 27367-27377 VBG denotes containing
T14601 27378-27381 DT denotes the
T14602 27382-27395 JJ denotes corresponding
T14603 27396-27402 NN denotes amount
T14604 27403-27405 IN denotes of
T14605 27406-27412 NN denotes rhIL-2
T14606 27412-27413 . denotes .
T15057 27415-27424 NN denotes Isolation
T15058 27425-27427 IN denotes of
T15059 27428-27431 CD denotes CD8
T15060 27431-27432 CD denotes +
T15061 27433-27434 NNP denotes T
T15062 27435-27440 NNS denotes cells
T15063 27441-27445 IN denotes from
T15064 27446-27451 JJ denotes Runx3
T15065 27451-27452 NN denotes −
T15066 27452-27453 NN denotes /
T15067 27453-27454 NN denotes −
T15068 27455-27459 NNS denotes mice
T15069 27459-27460 . denotes .
T15070 27461-27476 JJ denotes Runx3-deficient
T15071 27477-27478 NNP denotes T
T15072 27479-27484 NNS denotes cells
T15073 27485-27489 VBP denotes fail
T15074 27490-27492 TO denotes to
T15075 27493-27500 NN denotes silence
T15076 27501-27504 NNP denotes CD4
T15077 27505-27515 NN denotes expression
T15078 27516-27524 RB denotes normally
T15079 27525-27526 -LRB- denotes (
T15080 27526-27529 NNP denotes Fig
T15081 27529-27530 . denotes .
T15082 27531-27533 CD denotes S1
T15083 27533-27534 -RRB- denotes )
T15084 27535-27536 -LRB- denotes (
T15085 27536-27538 CD denotes 12
T15086 27538-27539 , denotes ,
T15087 27540-27542 CD denotes 13
T15088 27542-27543 -RRB- denotes )
T15089 27543-27544 . denotes .
T15090 27545-27547 PRP denotes We
T15091 27548-27557 RB denotes therefore
T15092 27558-27565 JJ denotes further
T15093 27566-27578 VBD denotes fractionated
T15094 27579-27582 DT denotes the
T15095 27583-27593 RB denotes positively
T15096 27594-27602 VBN denotes selected
T15097 27603-27606 NNP denotes CD8
T15098 27606-27607 CD denotes +
T15099 27608-27609 NNP denotes T
T15100 27610-27615 NNS denotes cells
T15101 27616-27620 IN denotes from
T15102 27621-27626 JJ denotes Runx3
T15103 27627-27629 NNP denotes KO
T15104 27630-27634 NNS denotes mice
T15105 27635-27639 IN denotes into
T15106 27640-27643 CD denotes CD8
T15107 27643-27644 CD denotes +
T15108 27644-27647 NNP denotes CD4
T15109 27647-27648 NNP denotes −
T15110 27649-27651 NNP denotes SP
T15111 27652-27654 CC denotes or
T15112 27655-27658 NNP denotes CD8
T15113 27658-27659 NNP denotes +
T15114 27659-27662 NNP denotes CD4
T15115 27662-27663 NNP denotes +
T15116 27664-27666 NNP denotes DP
T15117 27667-27672 NNS denotes cells
T15118 27673-27675 IN denotes by
T15119 27676-27686 NN denotes separation
T15120 27687-27692 VBG denotes using
T15121 27693-27701 JJ denotes anti-CD4
T15122 27702-27710 JJ denotes magnetic
T15123 27711-27716 NNS denotes beads
T15124 27716-27717 . denotes .
T15125 27718-27722 DT denotes This
T15126 27723-27730 VBD denotes yielded
T15127 27731-27732 DT denotes a
T15128 27733-27738 JJ denotes Runx3
T15129 27739-27741 NNP denotes KO
T15130 27742-27744 NNP denotes SP
T15131 27746-27754 VBD denotes enriched
T15132 27756-27766 NN denotes population
T15133 27767-27771 WDT denotes that
T15134 27772-27781 VBD denotes contained
T15135 27782-27784 CD denotes 75
T15136 27784-27785 NN denotes %
T15137 27786-27789 CD denotes CD8
T15138 27789-27790 NN denotes +
T15139 27790-27793 CD denotes CD4
T15140 27793-27794 NN denotes −
T15141 27795-27800 NNS denotes cells
T15142 27801-27804 CC denotes and
T15143 27805-27806 DT denotes a
T15144 27807-27809 NNP denotes KO
T15145 27810-27812 NNP denotes DP
T15146 27813-27821 VBD denotes enriched
T15147 27822-27832 NN denotes population
T15148 27833-27837 WDT denotes that
T15149 27838-27847 VBD denotes contained
T15150 27848-27850 CD denotes 85
T15151 27850-27851 NN denotes %
T15152 27852-27855 CD denotes CD8
T15153 27855-27856 NN denotes +
T15154 27856-27859 CD denotes CD4
T15155 27859-27860 NN denotes +
T15156 27861-27866 NNS denotes cells
T15157 27867-27868 -LRB- denotes (
T15158 27868-27871 NNP denotes Fig
T15159 27871-27872 . denotes .
T15160 27873-27875 CD denotes S1
T15161 27875-27876 -RRB- denotes )
T15162 27876-27877 . denotes .
T15163 27878-27881 DT denotes The
T15164 27882-27887 NNS denotes cells
T15165 27888-27892 VBD denotes were
T15166 27893-27903 VBN denotes stimulated
T15167 27904-27908 IN denotes with
T15168 27909-27917 JJ denotes anti-CD3
T15169 27917-27918 NN denotes +
T15170 27919-27928 NN denotes anti-CD28
T15171 27929-27932 IN denotes for
T15172 27933-27934 CD denotes 2
T15173 27935-27936 LS denotes d
T15174 27937-27943 IN denotes before
T15175 27944-27952 VBG denotes removing
T15176 27953-27957 PRP denotes them
T15177 27958-27962 IN denotes from
T15178 27963-27966 DT denotes the
T15179 27967-27970 NNP denotes TCR
T15180 27971-27979 NN denotes stimulus
T15181 27980-27983 CC denotes and
T15182 27984-27993 VBG denotes culturing
T15183 27994-27998 PRP denotes them
T15184 27999-28001 IN denotes in
T15185 28002-28007 NNS denotes media
T15186 28008-28018 VBG denotes containing
T15187 28019-28022 CD denotes 100
T15188 28023-28027 NNP denotes U/ml
T15189 28028-28032 NNP denotes IL-2
T15190 28032-28033 . denotes .
T15191 28034-28036 IN denotes As
T15192 28037-28047 RB denotes previously
T15193 28048-28056 VBN denotes reported
T15194 28056-28057 , denotes ,
T15195 28058-28069 JJ denotes TCR-induced
T15196 28070-28083 NN denotes proliferation
T15197 28084-28086 IN denotes of
T15198 28087-28092 NNP denotes Runx3
T15199 28092-28093 FW denotes −
T15200 28093-28094 FW denotes /
T15201 28094-28095 FW denotes −
T15202 28096-28099 FW denotes CD8
T15203 28099-28100 FW denotes +
T15204 28101-28102 FW denotes T
T15205 28103-28108 NNS denotes cells
T15206 28109-28112 VBD denotes was
T15207 28113-28121 RB denotes severely
T15208 28122-28130 VBN denotes impaired
T15209 28130-28131 , denotes ,
T15210 28132-28144 RB denotes irrespective
T15211 28145-28147 IN denotes of
T15212 28148-28151 CD denotes CD4
T15213 28152-28162 NN denotes expression
T15214 28163-28164 -LRB- denotes (
T15215 28164-28167 NNP denotes Fig
T15216 28167-28168 . denotes .
T15217 28169-28171 CD denotes S1
T15218 28171-28172 -RRB- denotes )
T15219 28173-28174 -LRB- denotes (
T15220 28174-28176 CD denotes 12
T15221 28176-28177 , denotes ,
T15222 28178-28180 CD denotes 13
T15223 28180-28181 -RRB- denotes )
T15224 28181-28182 . denotes .
T15225 28183-28190 RB denotes However
T15226 28190-28191 , denotes ,
T15227 28192-28195 DT denotes the
T15228 28196-28201 NNP denotes Runx3
T15229 28201-28202 NNP denotes −
T15230 28202-28203 VBD denotes /
T15231 28203-28204 CD denotes −
T15232 28205-28210 NNS denotes cells
T15233 28211-28217 VBD denotes showed
T15234 28218-28230 JJ denotes cell-surface
T15235 28231-28241 NN denotes expression
T15236 28242-28250 NNS denotes patterns
T15237 28251-28261 JJ denotes indicative
T15238 28262-28264 IN denotes of
T15239 28265-28274 VBN denotes activated
T15240 28275-28280 NNS denotes cells
T15241 28280-28281 , denotes ,
T15242 28282-28291 VBG denotes including
T15243 28292-28305 NN denotes up-regulation
T15244 28306-28308 IN denotes of
T15245 28309-28313 NNP denotes CD25
T15246 28314-28317 CC denotes and
T15247 28318-28322 NNP denotes CD69
T15248 28323-28324 -LRB- denotes (
T15249 28324-28327 NNP denotes Fig
T15250 28327-28328 . denotes .
T15251 28329-28331 CD denotes S1
T15252 28331-28332 -RRB- denotes )
T15253 28332-28333 . denotes .
T15254 28334-28336 IN denotes As
T15255 28337-28345 VBN denotes expected
T15256 28346-28350 IN denotes from
T15257 28351-28356 PRP$ denotes their
T15258 28357-28364 NN denotes ability
T15259 28365-28367 TO denotes to
T15260 28368-28379 VB denotes up-regulate
T15261 28380-28384 NNP denotes CD25
T15262 28384-28385 , denotes ,
T15263 28386-28391 NNP denotes Runx3
T15264 28391-28392 FW denotes −
T15265 28392-28393 FW denotes /
T15266 28393-28394 FW denotes −
T15267 28395-28398 FW denotes CD8
T15268 28398-28399 FW denotes +
T15269 28400-28401 FW denotes T
T15270 28402-28407 NNS denotes cells
T15271 28408-28417 VBD denotes responded
T15272 28418-28420 TO denotes to
T15273 28421-28425 NNP denotes IL-2
T15274 28426-28441 NN denotes supplementation
T15275 28442-28447 IN denotes after
T15276 28448-28451 NN denotes day
T15277 28452-28453 CD denotes 2
T15278 28454-28457 CC denotes and
T15279 28458-28469 RB denotes efficiently
T15280 28470-28478 VBN denotes expanded
T15281 28479-28484 IN denotes until
T15282 28485-28488 NN denotes day
T15283 28489-28490 CD denotes 6
T15284 28491-28493 IN denotes of
T15285 28494-28497 DT denotes the
T15286 28498-28505 NN denotes culture
T15287 28506-28512 NN denotes period
T15288 28512-28513 , denotes ,
T15289 28514-28520 IN denotes albeit
T15290 28521-28523 IN denotes at
T15291 28524-28530 JJR denotes slower
T15292 28531-28536 NNS denotes rates
T15293 28537-28545 VBN denotes compared
T15294 28546-28550 IN denotes with
T15295 28551-28553 NNP denotes WT
T15296 28554-28559 NNS denotes cells
T15297 28560-28561 -LRB- denotes (
T15298 28561-28564 NNP denotes Fig
T15299 28564-28565 . denotes .
T15300 28566-28568 CD denotes S1
T15301 28568-28569 -RRB- denotes )
T15302 28569-28570 . denotes .
T15303 28571-28579 IN denotes Although
T15304 28580-28581 DT denotes a
T15305 28582-28590 NN denotes fraction
T15306 28591-28593 IN denotes of
T15307 28594-28597 DT denotes the
T15308 28598-28600 NNP denotes KO
T15309 28601-28603 NNP denotes DP
T15310 28604-28609 NNS denotes cells
T15311 28610-28618 VBD denotes silenced
T15312 28619-28622 CD denotes CD4
T15313 28623-28633 NN denotes expression
T15314 28634-28639 IN denotes after
T15315 28640-28650 NN denotes activation
T15316 28650-28651 , denotes ,
T15317 28652-28655 DT denotes the
T15318 28656-28661 NN denotes ratio
T15319 28662-28664 IN denotes of
T15320 28665-28670 NNP denotes SP/DP
T15321 28671-28676 NNS denotes cells
T15322 28677-28679 IN denotes in
T15323 28680-28684 DT denotes each
T15324 28685-28693 VBN denotes enriched
T15325 28694-28704 NN denotes population
T15326 28705-28713 VBD denotes remained
T15327 28714-28722 JJ denotes constant
T15328 28723-28733 RB denotes thereafter
T15329 28733-28734 , denotes ,
T15330 28735-28738 CC denotes and
T15331 28739-28741 PRP denotes we
T15332 28742-28745 VBD denotes did
T15333 28746-28749 RB denotes not
T15334 28750-28757 VB denotes observe
T15335 28758-28761 DT denotes any
T15336 28762-28767 JJ denotes major
T15337 28768-28779 NNS denotes differences
T15338 28780-28787 IN denotes between
T15339 28788-28793 DT denotes these
T15340 28794-28797 CD denotes two
T15341 28798-28809 NNS denotes populations
T15342 28810-28820 IN denotes throughout
T15343 28821-28824 DT denotes the
T15344 28825-28832 NN denotes culture
T15345 28833-28839 NN denotes period
T15346 28839-28840 , denotes ,
T15347 28841-28851 VBG denotes indicating
T15348 28852-28856 IN denotes that
T15349 28857-28859 IN denotes in
T15350 28860-28865 NNS denotes terms
T15351 28866-28868 IN denotes of
T15352 28869-28877 NN denotes effector
T15353 28878-28881 NNP denotes CTL
T15354 28882-28897 NN denotes differentiation
T15355 28898-28901 CC denotes and
T15356 28902-28907 IN denotes under
T15357 28908-28911 PRP$ denotes our
T15358 28912-28919 NN denotes culture
T15359 28920-28930 NNS denotes conditions
T15360 28930-28931 , denotes ,
T15361 28932-28937 JJ denotes Runx3
T15362 28937-28938 FW denotes −
T15363 28938-28939 FW denotes /
T15364 28939-28940 FW denotes −
T15365 28941-28944 FW denotes CD8
T15366 28944-28945 FW denotes +
T15367 28946-28947 FW denotes T
T15368 28948-28953 NNS denotes cells
T15369 28954-28958 WDT denotes that
T15370 28959-28963 RB denotes also
T15371 28964-28973 VBZ denotes coexpress
T15372 28974-28977 CD denotes CD4
T15373 28978-28981 VBP denotes are
T15374 28982-28999 JJ denotes indistinguishable
T15375 29000-29004 IN denotes from
T15376 29005-29010 DT denotes those
T15377 29011-29015 WDT denotes that
T15378 29016-29018 VBP denotes do
T15379 29019-29022 RB denotes not
T15380 29022-29023 . denotes .
T15381 29024-29027 DT denotes The
T15382 29028-29032 NNS denotes data
T15383 29033-29042 VBN denotes presented
T15384 29043-29045 IN denotes in
T15385 29046-29049 NNP denotes Fig
T15386 29049-29050 . denotes .
T15387 29051-29053 CD denotes S2
T15388 29054-29057 VBP denotes are
T15389 29058-29062 IN denotes from
T15390 29063-29068 JJ denotes Runx3
T15391 29069-29071 NNP denotes KO
T15392 29072-29074 NNP denotes SP
T15393 29075-29080 NNS denotes cells
T15394 29080-29081 , denotes ,
T15395 29082-29089 IN denotes whereas
T15396 29090-29095 DT denotes those
T15397 29096-29101 VBN denotes shown
T15398 29102-29104 IN denotes in
T15399 29105-29110 NNP denotes Figs.
T15400 29111-29112 CD denotes 3
T15401 29113-29116 CC denotes and
T15402 29117-29118 CD denotes 4
T15403 29119-29122 VBP denotes are
T15404 29123-29127 IN denotes from
T15405 29128-29133 JJ denotes total
T15406 29134-29139 JJ denotes Runx3
T15407 29140-29142 NNP denotes KO
T15408 29143-29146 NNP denotes CD8
T15409 29147-29152 NNS denotes cells
T15410 29152-29153 . denotes .
T16130 29155-29165 JJ denotes FACS-based
T16131 29166-29178 NN denotes cytotoxicity
T16132 29179-29184 NN denotes assay
T16133 29184-29185 . denotes .
T16134 29186-29188 TO denotes To
T16135 29189-29196 VB denotes measure
T16136 29197-29209 NN denotes cytotoxicity
T16137 29209-29210 , denotes ,
T16138 29211-29214 CD denotes EL4
T16139 29215-29222 NN denotes thymoma
T16140 29223-29229 NN denotes target
T16141 29230-29235 NNS denotes cells
T16142 29236-29240 VBD denotes were
T16143 29241-29247 VBN denotes loaded
T16144 29248-29252 IN denotes with
T16145 29253-29254 CD denotes 0
T16146 29255-29257 CC denotes or
T16147 29258-29259 CD denotes 1
T16148 29260-29262 NNP denotes μM
T16149 29263-29267 NNP denotes Gp33
T16150 29268-29275 NN denotes peptide
T16151 29276-29279 IN denotes for
T16152 29280-29281 CD denotes 2
T16153 29282-29283 NN denotes h
T16154 29284-29290 IN denotes before
T16155 29291-29292 DT denotes a
T16156 29293-29296 JJ denotes 2-h
T16157 29297-29309 NN denotes coincubation
T16158 29310-29314 IN denotes with
T16159 29315-29318 NNP denotes P14
T16160 29319-29322 NNP denotes CD8
T16161 29322-29323 VBD denotes +
T16162 29324-29325 NNP denotes T
T16163 29326-29331 NNS denotes cells
T16164 29332-29334 IN denotes at
T16165 29335-29338 DT denotes the
T16166 29339-29357 JJ denotes effector-to-target
T16167 29358-29364 NNS denotes ratios
T16168 29365-29374 VBD denotes indicated
T16169 29375-29377 IN denotes in
T16170 29378-29381 DT denotes the
T16171 29382-29389 NNS denotes figures
T16172 29390-29392 IN denotes in
T16173 29393-29400 JJ denotes 96-well
T16174 29401-29413 NN denotes round-bottom
T16175 29414-29420 NNS denotes plates
T16176 29420-29421 . denotes .
T16177 29422-29427 IN denotes After
T16178 29428-29431 DT denotes the
T16179 29432-29444 NN denotes coincubation
T16180 29445-29451 NN denotes period
T16181 29451-29452 , denotes ,
T16182 29453-29458 NNS denotes cells
T16183 29459-29463 VBD denotes were
T16184 29464-29471 VBN denotes stained
T16185 29472-29476 IN denotes with
T16186 29477-29484 NNP denotes Annexin
T16187 29485-29486 NNP denotes V
T16188 29487-29491 NNP denotes FITC
T16189 29492-29495 CC denotes and
T16190 29496-29504 JJ denotes anti-CD8
T16191 29505-29520 NN denotes allophycocyanin
T16192 29520-29521 . denotes .
T16193 29522-29526 NNP denotes Data
T16194 29527-29535 NN denotes analysis
T16195 29536-29539 VBD denotes was
T16196 29540-29549 VBN denotes performed
T16197 29550-29554 IN denotes with
T16198 29555-29561 NNP denotes FlowJo
T16199 29562-29570 NN denotes software
T16200 29571-29572 -LRB- denotes (
T16201 29572-29576 NNP denotes Tree
T16202 29577-29581 NNP denotes Star
T16203 29581-29582 , denotes ,
T16204 29583-29587 NNP denotes Inc.
T16205 29587-29588 -RRB- denotes )
T16206 29588-29589 : denotes ;
T16207 29590-29593 CD denotes EL4
T16208 29594-29600 NN denotes target
T16209 29601-29606 NNS denotes cells
T16210 29607-29608 -LRB- denotes (
T16211 29608-29620 JJ denotes CD8-negative
T16212 29621-29627 NNS denotes events
T16213 29627-29628 -RRB- denotes )
T16214 29629-29633 VBD denotes were
T16215 29634-29639 VBN denotes gated
T16216 29639-29640 , denotes ,
T16217 29641-29644 CC denotes and
T16218 29645-29648 DT denotes the
T16219 29649-29659 NN denotes percentage
T16220 29660-29662 IN denotes of
T16221 29663-29670 NNP denotes Annexin
T16222 29671-29672 NNP denotes V
T16223 29672-29673 VBD denotes +
T16224 29674-29680 NN denotes target
T16225 29681-29686 NNS denotes cells
T16226 29687-29690 VBD denotes was
T16227 29691-29701 VBN denotes determined
T16228 29701-29702 . denotes .
T16385 29704-29712 NNP denotes Cytokine
T16386 29713-29716 CC denotes and
T16387 29717-29724 NN denotes surface
T16388 29725-29731 NN denotes marker
T16389 29732-29740 VBG denotes staining
T16390 29740-29741 . denotes .
T16391 29742-29744 TO denotes To
T16392 29745-29751 VB denotes assess
T16393 29752-29760 JJ denotes cytokine
T16394 29761-29771 NN denotes production
T16395 29771-29772 , denotes ,
T16396 29773-29778 NNS denotes cells
T16397 29779-29783 VBD denotes were
T16398 29784-29796 VBN denotes restimulated
T16399 29797-29801 IN denotes with
T16400 29802-29804 CD denotes 10
T16401 29805-29807 NNP denotes nM
T16402 29808-29811 NNP denotes PMA
T16403 29812-29813 VBD denotes +
T16404 29814-29815 CD denotes 1
T16405 29816-29818 NNP denotes μM
T16406 29819-29828 NN denotes ionomycin
T16407 29829-29832 IN denotes for
T16408 29833-29834 CD denotes 6
T16409 29835-29836 NN denotes h
T16410 29837-29838 -LRB- denotes (
T16411 29838-29844 IN denotes unless
T16412 29845-29854 VBN denotes indicated
T16413 29855-29864 RB denotes otherwise
T16414 29865-29867 IN denotes in
T16415 29868-29871 DT denotes the
T16416 29872-29879 NNS denotes figures
T16417 29879-29880 -RRB- denotes )
T16418 29880-29881 , denotes ,
T16419 29882-29885 CC denotes and
T16420 29886-29899 JJ denotes intracellular
T16421 29900-29908 NN denotes cytokine
T16422 29909-29915 NNS denotes stains
T16423 29916-29920 VBD denotes were
T16424 29921-29930 VBN denotes performed
T16425 29931-29933 IN denotes as
T16426 29934-29944 RB denotes previously
T16427 29945-29954 VBN denotes described
T16428 29955-29956 -LRB- denotes (
T16429 29956-29958 CD denotes 28
T16430 29958-29959 -RRB- denotes )
T16431 29959-29960 . denotes .
T16432 29961-29963 TO denotes To
T16433 29964-29970 VB denotes detect
T16434 29971-29981 NN denotes expression
T16435 29982-29984 IN denotes of
T16436 29985-29992 NN denotes surface
T16437 29993-30002 NNS denotes molecules
T16438 30002-30003 , denotes ,
T16439 30004-30009 NNS denotes cells
T16440 30010-30014 VBD denotes were
T16441 30015-30021 VBN denotes washed
T16442 30022-30024 IN denotes in
T16443 30025-30028 NNP denotes PBS
T16444 30028-30029 , denotes ,
T16445 30030-30041 VBD denotes resuspended
T16446 30042-30044 IN denotes in
T16447 30045-30049 NNP denotes FACS
T16448 30050-30054 VBP denotes wash
T16449 30055-30061 NN denotes buffer
T16450 30062-30063 -LRB- denotes (
T16451 30063-30064 CD denotes 3
T16452 30064-30065 NN denotes %
T16453 30066-30069 NNP denotes FBS
T16454 30069-30070 , denotes ,
T16455 30071-30074 CD denotes 0.1
T16456 30074-30075 NN denotes %
T16457 30076-30082 NN denotes sodium
T16458 30083-30088 NN denotes azide
T16459 30088-30089 , denotes ,
T16460 30090-30092 CD denotes 30
T16461 30093-30095 NNP denotes mM
T16462 30096-30101 NNP denotes Hepes
T16463 30101-30102 , denotes ,
T16464 30103-30104 CD denotes 1
T16465 30104-30105 CD denotes ×
T16466 30106-30109 NNP denotes PBS
T16467 30109-30110 -RRB- denotes )
T16468 30111-30121 VBG denotes containing
T16469 30122-30125 DT denotes the
T16470 30126-30136 NNS denotes antibodies
T16471 30137-30146 VBD denotes indicated
T16472 30147-30149 IN denotes in
T16473 30150-30153 DT denotes the
T16474 30154-30161 NNS denotes figures
T16475 30162-30164 IN denotes at
T16476 30165-30175 RB denotes previously
T16477 30176-30185 VBN denotes optimized
T16478 30186-30200 NNS denotes concentrations
T16479 30200-30201 , denotes ,
T16480 30202-30211 VBN denotes incubated
T16481 30212-30215 IN denotes for
T16482 30216-30218 CD denotes 15
T16483 30219-30222 NN denotes min
T16484 30223-30225 IN denotes at
T16485 30226-30230 NN denotes room
T16486 30231-30242 NN denotes temperature
T16487 30243-30244 -LRB- denotes (
T16488 30244-30246 NNP denotes RT
T16489 30246-30247 -RRB- denotes )
T16490 30247-30248 , denotes ,
T16491 30249-30255 VBD denotes washed
T16492 30255-30256 , denotes ,
T16493 30257-30260 CC denotes and
T16494 30261-30272 VBD denotes resuspended
T16495 30273-30275 IN denotes in
T16496 30276-30277 CD denotes 2
T16497 30277-30278 NN denotes %
T16498 30279-30291 NN denotes formaldehyde
T16499 30292-30300 JJ denotes fixative
T16500 30301-30309 NN denotes solution
T16501 30310-30316 IN denotes before
T16502 30317-30328 NN denotes acquisition
T16503 30329-30331 IN denotes on
T16504 30332-30333 DT denotes a
T16505 30334-30345 NNP denotes FACSCalibur
T16506 30346-30347 -LRB- denotes (
T16507 30347-30349 NNP denotes BD
T16508 30349-30350 -RRB- denotes )
T16509 30350-30351 . denotes .
T16656 30353-30363 NNP denotes Retroviral
T16657 30364-30376 NN denotes transduction
T16658 30377-30379 IN denotes of
T16659 30380-30387 JJ denotes primary
T16660 30388-30391 NNP denotes CD8
T16661 30391-30392 CD denotes +
T16662 30393-30394 NNP denotes T
T16663 30395-30400 NNS denotes cells
T16664 30400-30401 . denotes .
T16665 30402-30405 IN denotes For
T16666 30406-30418 NN denotes transduction
T16667 30419-30430 NNS denotes experiments
T16668 30430-30431 , denotes ,
T16669 30432-30437 JJ denotes viral
T16670 30438-30450 NNS denotes supernatants
T16671 30451-30455 VBD denotes were
T16672 30456-30465 VBN denotes generated
T16673 30466-30468 IN denotes by
T16674 30469-30476 NN denotes calcium
T16675 30477-30486 NN denotes phosphate
T16676 30487-30499 NN denotes transfection
T16677 30500-30502 IN denotes of
T16678 30503-30510 NNP denotes Phoenix
T16679 30511-30516 NNS denotes cells
T16680 30517-30520 CC denotes and
T16681 30521-30534 NN denotes concentration
T16682 30535-30537 IN denotes by
T16683 30538-30547 JJ denotes overnight
T16684 30548-30562 NN denotes centrifugation
T16685 30563-30565 IN denotes at
T16686 30566-30571 CD denotes 6,000
T16687 30572-30573 NN denotes g
T16688 30573-30574 . denotes .
T16689 30575-30577 IN denotes At
T16690 30578-30579 CD denotes ∼
T16691 30579-30581 CD denotes 42
T16692 30582-30583 NN denotes h
T16693 30584-30589 IN denotes after
T16694 30590-30593 DT denotes the
T16695 30594-30601 JJ denotes initial
T16696 30602-30605 NNP denotes TCR
T16697 30606-30616 NN denotes activation
T16698 30617-30619 IN denotes of
T16699 30620-30623 CD denotes 106
T16700 30624-30627 CD denotes CD8
T16701 30627-30628 NN denotes +
T16702 30629-30630 NNP denotes T
T16703 30631-30636 NNS denotes cells
T16721 30741-30745 IN denotes with
T16722 30746-30747 CD denotes 8
T16723 30748-30750 NN denotes μg
T16724 30750-30751 NN denotes /
T16725 30751-30753 NN denotes ml
T16726 30754-30763 NN denotes polybrene
T16727 30764-30774 VBG denotes containing
T16728 30775-30780 JJ denotes fresh
T16729 30781-30785 CC denotes plus
T16730 30786-30798 JJ denotes concentrated
T16731 30799-30804 NN denotes virus
T16732 30804-30805 . denotes .
T16733 30806-30809 DT denotes The
T16734 30810-30816 NNS denotes plates
T16735 30817-30821 VBD denotes were
T16736 30822-30833 VBN denotes centrifuged
T16737 30834-30836 IN denotes at
T16738 30837-30840 CD denotes 700
T16739 30841-30842 NN denotes g
T16740 30843-30846 IN denotes for
T16741 30847-30848 CD denotes 1
T16742 30849-30850 NN denotes h
T16743 30851-30853 IN denotes at
T16744 30854-30856 NNP denotes RT
T16745 30857-30863 IN denotes before
T16746 30864-30873 VBG denotes returning
T16747 30874-30876 TO denotes to
T16748 30877-30879 CD denotes 37
T16749 30879-30880 CD denotes °
T16750 30880-30881 NNP denotes C
T16751 30882-30885 IN denotes for
T16752 30886-30888 DT denotes an
T16753 30889-30899 JJ denotes additional
T16754 30900-30901 CD denotes 5
T16755 30902-30904 NN denotes h.
T16756 30905-30915 NNP denotes Retroviral
T16757 30916-30926 NNS denotes constructs
T16758 30927-30930 IN denotes for
T16759 30931-30941 NNP denotes Eomes-VP16
T16760 30942-30945 CC denotes and
T16761 30946-30949 DT denotes the
T16762 30950-30953 NNP denotes MIG
T16763 30954-30961 NN denotes control
T16764 30962-30967 JJ denotes empty
T16765 30968-30974 NN denotes vector
T16766 30975-30979 VBD denotes were
T16767 30980-30981 DT denotes a
T16768 30982-30986 NN denotes gift
T16769 30987-30991 IN denotes from
T16770 30992-30996 NNP denotes S.L.
T16771 30997-31003 NNP denotes Reiner
T16772 31004-31005 -LRB- denotes (
T16773 31005-31015 NNP denotes University
T16774 31016-31018 IN denotes of
T16775 31019-31031 NNP denotes Pennsylvania
T16776 31031-31032 , denotes ,
T16777 31033-31045 NNP denotes Philadelphia
T16778 31045-31046 , denotes ,
T16779 31047-31049 NNP denotes PA
T16780 31049-31050 -RRB- denotes )
T16781 31051-31052 -LRB- denotes (
T16782 31052-31053 CD denotes 8
T16783 31053-31054 -RRB- denotes )
T16784 31054-31055 . denotes .
T17610 33821-33826 NN denotes nylon
T17611 33827-33836 NNS denotes membranes
T17612 33837-33838 -LRB- denotes (
T17613 33838-33846 JJ denotes Hybond-N
T17614 33846-33847 NN denotes +
T17615 33847-33848 : denotes ;
T17616 33849-33851 NNP denotes GE
T17617 33852-33862 NNP denotes Healthcare
T17618 33862-33863 -RRB- denotes )
T17619 33863-33864 , denotes ,
T17620 33865-33870 WDT denotes which
T17621 33871-33874 VBD denotes was
T17622 33875-33884 VBN denotes confirmed
T17623 33885-33887 IN denotes by
T17624 33888-33896 NN denotes ethidium
T17625 33897-33904 NN denotes bromide
T17626 33905-33913 NN denotes staining
T17627 33914-33916 IN denotes of
T17628 33917-33926 JJ denotes ribosomal
T17629 33927-33930 NNP denotes RNA
T17630 33931-33938 NNS denotes species
T17631 33939-33941 IN denotes on
T17632 33942-33945 DT denotes the
T17633 33946-33954 NN denotes membrane
T17634 33954-33955 . denotes .
T17635 33956-33965 NNS denotes Membranes
T17636 33966-33970 VBD denotes were
T17637 33971-33981 VBN denotes hybridized
T17638 33982-33986 IN denotes with
T17639 33987-33988 CD denotes 1
T17640 33989-33994 NN denotes ng/ml
T17641 33995-33996 SYM denotes α
T17642 33996-33997 : denotes -
T17643 33997-33998 NNP denotes [
T17644 33998-34001 NNP denotes 32P
T17645 34001-34002 NNP denotes ]
T17646 34002-34006 NNP denotes dCTP
T17647 34007-34014 VBN denotes labeled
T17648 34015-34030 JJ denotes trichloroacetic
T17649 34031-34035 NN denotes acid
T17650 34036-34048 JJ denotes precipitable
T17651 34049-34054 NN denotes probe
T17652 34055-34057 IN denotes in
T17653 34058-34068 NNP denotes ExpressHyb
T17654 34069-34082 NN denotes hybridization
T17655 34083-34089 NN denotes buffer
T17656 34090-34091 -LRB- denotes (
T17657 34091-34099 NNP denotes Clontech
T17658 34100-34112 NNPS denotes Laboratories
T17659 34112-34113 , denotes ,
T17660 34114-34118 NNP denotes Inc.
T17661 34118-34119 -RRB- denotes )
T17662 34119-34120 . denotes .
T17663 34121-34124 DT denotes All
T17664 34125-34129 NNP denotes cDNA
T17665 34130-34136 NNS denotes probes
T17666 34137-34141 VBD denotes were
T17667 34142-34151 VBN denotes confirmed
T17668 34152-34154 TO denotes to
T17669 34155-34159 VB denotes have
T17670 34160-34163 DT denotes the
T17671 34164-34175 JJ denotes appropriate
T17672 34176-34187 JJ denotes single-copy
T17673 34188-34199 NN denotes specificity
T17674 34200-34205 IN denotes under
T17675 34206-34211 DT denotes these
T17676 34212-34222 NNS denotes conditions
T17677 34223-34228 VBG denotes using
T17678 34229-34236 JJ denotes genomic
T17679 34237-34245 NNP denotes Southern
T17680 34246-34250 NN denotes blot
T17681 34251-34259 NN denotes analysis
T17682 34259-34260 . denotes .
T17683 34261-34265 NNP denotes Band
T17684 34266-34277 NNS denotes intensities
T17685 34278-34282 VBD denotes were
T17686 34283-34291 VBN denotes acquired
T17687 34292-34294 IN denotes by
T17688 34295-34310 VBG denotes phosphorimaging
T17689 34311-34319 NN denotes analysis
T17690 34319-34320 . denotes .
T17691 34321-34324 IN denotes For
T17692 34325-34332 JJ denotes Western
T17693 34333-34341 NN denotes analysis
T17694 34341-34342 , denotes ,
T17695 34343-34353 JJ denotes whole-cell
T17696 34354-34361 NN denotes protein
T17697 34362-34369 NNS denotes lysates
T17698 34370-34374 VBD denotes were
T17699 34375-34383 VBN denotes obtained
T17700 34384-34388 IN denotes from
T17701 34389-34392 CD denotes CD8
T17702 34392-34393 CD denotes +
T17703 34394-34395 NNP denotes T
T17704 34396-34401 NNS denotes cells
T17705 34402-34404 IN denotes at
T17706 34405-34408 DT denotes the
T17707 34409-34413 NN denotes time
T17708 34414-34420 NNS denotes points
T17709 34421-34430 VBD denotes indicated
T17710 34431-34433 IN denotes in
T17711 34434-34437 DT denotes the
T17712 34438-34445 NNS denotes figures
T17713 34446-34452 IN denotes during
T17714 34453-34459 JJ denotes clonal
T17715 34460-34469 NN denotes expansion
T17716 34470-34472 IN denotes in
T17717 34473-34476 CD denotes 100
T17718 34477-34481 NNP denotes U/ml
T17719 34482-34486 NN denotes IL-2
T17720 34487-34491 IN denotes with
T17721 34492-34497 NN denotes lysis
T17722 34498-34504 NN denotes buffer
T17723 34505-34506 -LRB- denotes (
T17724 34506-34508 CD denotes 50
T17725 34509-34511 NNP denotes mM
T17726 34512-34516 NNP denotes Tris
T17727 34517-34518 NNP denotes [
T17728 34518-34520 NNP denotes pH
T17729 34521-34524 CD denotes 7.5
T17730 34524-34525 NNP denotes ]
T17731 34525-34526 , denotes ,
T17732 34527-34530 CD denotes 150
T17733 34531-34533 NNP denotes mM
T17734 34534-34538 NNP denotes NaCl
T17735 34538-34539 , denotes ,
T17736 34540-34542 CD denotes 10
T17737 34542-34543 NN denotes %
T17738 34544-34552 NN denotes glycerol
T17739 34552-34553 , denotes ,
T17740 34554-34555 CD denotes 5
T17741 34556-34558 NNP denotes mM
T17742 34559-34563 NNP denotes EDTA
T17743 34563-34564 , denotes ,
T17744 34565-34566 CD denotes 1
T17745 34566-34567 NN denotes %
T17746 34568-34573 NN denotes NP-40
T17747 34573-34574 -RRB- denotes )
T17748 34575-34577 IN denotes by
T17749 34578-34590 VBG denotes resuspending
T17750 34591-34598 NNS denotes samples
T17751 34599-34601 IN denotes in
T17752 34602-34604 CD denotes 10
T17753 34605-34607 NN denotes μl
T17754 34608-34611 IN denotes per
T17755 34612-34615 CD denotes 106
T17756 34616-34621 NNS denotes cells
T17757 34622-34625 CC denotes and
T17758 34626-34636 VBG denotes incubating
T17759 34637-34639 IN denotes on
T17760 34640-34643 NN denotes ice
T17761 34644-34647 IN denotes for
T17762 34648-34650 CD denotes 30
T17763 34651-34654 NN denotes min
T17764 34655-34657 IN denotes in
T17765 34658-34661 DT denotes the
T17766 34662-34670 NN denotes presence
T17767 34671-34673 IN denotes of
T17768 34674-34682 NN denotes protease
T17769 34683-34693 NNS denotes inhibitors
T17770 34693-34694 . denotes .
T17771 34695-34705 NNP denotes Immunoblot
T17772 34706-34714 NN denotes analysis
T17773 34715-34718 VBD denotes was
T17774 34719-34728 VBN denotes performed
T17775 34729-34733 IN denotes with
T17776 34734-34737 DT denotes the
T17777 34738-34748 NNS denotes antibodies
T17778 34749-34758 VBD denotes indicated
T17779 34759-34761 IN denotes in
T17780 34762-34765 DT denotes the
T17781 34766-34773 NNS denotes figures
T17782 34774-34779 IN denotes after
T17783 34780-34788 NNP denotes SDS-PAGE
T17784 34789-34790 -LRB- denotes (
T17785 34790-34792 CD denotes 10
T17786 34793-34795 CD denotes 30
T17787 34796-34798 NN denotes μg
T17788 34799-34801 IN denotes of
T17789 34802-34807 JJ denotes total
T17790 34808-34815 NN denotes protein
T17791 34816-34819 VBD denotes was
T17792 34820-34826 VBN denotes loaded
T17793 34827-34830 IN denotes per
T17794 34831-34835 NN denotes well
T17795 34835-34836 -RRB- denotes )
T17796 34836-34837 . denotes .
T17797 34838-34852 NN denotes Quantification
T17798 34853-34855 IN denotes of
T17799 34856-34864 VBN denotes detected
T17800 34865-34872 NN denotes protein
T17801 34873-34876 VBD denotes was
T17802 34877-34886 VBN denotes performed
T17803 34887-34891 IN denotes with
T17804 34892-34894 DT denotes an
T17805 34895-34906 NNP denotes Intelligent
T17806 34907-34911 JJ denotes Dark
T17807 34912-34915 NNP denotes Box
T17808 34916-34920 NN denotes unit
T17809 34921-34922 -LRB- denotes (
T17810 34922-34930 NN denotes LAS-3000
T17811 34930-34931 : denotes ;
T17812 34932-34940 NNP denotes Fujifilm
T17813 34940-34941 -RRB- denotes )
T17814 34942-34945 CC denotes and
T17815 34946-34956 VBN denotes normalized
T17816 34957-34960 IN denotes for
T17817 34961-34968 VBG denotes loading
T17818 34969-34973 IN denotes with
T17819 34974-34977 DT denotes the
T17820 34978-34984 NN denotes amount
T17821 34985-34987 IN denotes of
T17822 34988-34991 NNP denotes RNA
T17823 34992-34998 NNP denotes Pol-II
T17824 34999-35007 VBD denotes detected
T17825 35008-35010 IN denotes in
T17826 35011-35015 DT denotes each
T17827 35016-35020 NN denotes lane
T17828 35020-35021 . denotes .
T18126 35023-35029 NNP denotes Online
T18127 35030-35042 JJ denotes supplemental
T18128 35043-35051 NN denotes material
T18129 35051-35052 . denotes .
T18130 35053-35056 NNP denotes Fig
T18131 35056-35057 . denotes .
T18132 35058-35060 CD denotes S1
T18133 35061-35066 VBZ denotes shows
T18134 35067-35070 DT denotes the
T18135 35071-35087 NN denotes characterization
T18136 35088-35090 IN denotes of
T18137 35091-35101 JJ denotes peripheral
T18138 35102-35105 NNP denotes CD8
T18139 35105-35106 CD denotes +
T18140 35107-35108 NNP denotes T
T18141 35109-35114 NNS denotes cells
T18142 35115-35119 IN denotes from
T18143 35120-35125 JJ denotes Runx3
T18144 35125-35126 NN denotes −
T18145 35126-35127 NN denotes /
T18146 35127-35128 NN denotes −
T18147 35129-35133 NNS denotes mice
T18148 35133-35134 . denotes .
T18149 35135-35138 NNP denotes Fig
T18150 35138-35139 . denotes .
T18151 35140-35142 CD denotes S2
T18152 35143-35148 NNS denotes shows
T18153 35149-35157 JJ denotes effector
T18154 35158-35165 NN denotes protein
T18155 35166-35176 NN denotes expression
T18156 35177-35179 IN denotes by
T18157 35180-35185 NNP denotes Runx3
T18158 35186-35188 NNP denotes WT
T18159 35189-35192 CC denotes and
T18160 35193-35195 NNP denotes KO
T18161 35196-35201 NNS denotes cells
T18162 35202-35204 IN denotes at
T18163 35205-35208 NN denotes day
T18164 35209-35210 CD denotes 4
T18165 35211-35213 IN denotes of
T18166 35214-35216 IN denotes in
T18167 35217-35222 NN denotes vitro
T18168 35223-35230 NN denotes culture
T18169 35230-35231 . denotes .
T18170 35232-35238 NNP denotes Primer
T18171 35239-35248 NNS denotes sequences
T18172 35249-35253 VBD denotes used
T18173 35254-35257 IN denotes for
T18174 35258-35262 NNP denotes ChIP
T18175 35263-35274 NNS denotes experiments
T18176 35275-35278 VBP denotes are
T18177 35279-35284 VBN denotes shown
T18178 35285-35287 IN denotes in
T18179 35288-35293 NNP denotes Table
T18180 35294-35296 NNP denotes S1
T18181 35296-35297 . denotes .
T18182 35298-35304 NNP denotes Online
T18183 35305-35317 JJ denotes supplemental
T18184 35318-35326 NN denotes material
T18185 35327-35329 VBZ denotes is
T18186 35330-35339 JJ denotes available
T18187 35340-35342 IN denotes at
T18188 35343-35395 NNP denotes http://www.jem.org/cgi/content/full/jem.20081242/DC1
T18189 35395-35396 . denotes .
T18364 6546-6554 NNS denotes Kinetics
T18365 6555-6557 IN denotes of
T18366 6558-6562 NN denotes gene
T18367 6563-6573 NN denotes expression
T18368 6574-6580 IN denotes during
T18369 6581-6584 NNP denotes CD8
T18370 6584-6585 NNP denotes +
T18371 6586-6587 NNP denotes T
T18372 6588-6592 NN denotes cell
T18373 6593-6608 NN denotes differentiation
T18374 6608-6609 . denotes .
T18375 6610-6611 -LRB- denotes (
T18376 6611-6612 NNP denotes A
T18377 6612-6613 -RRB- denotes )
T18378 6614-6622 NNPS denotes Kinetics
T18379 6623-6625 IN denotes of
T18380 6626-6630 NNP denotes Prf1
T18381 6630-6631 , denotes ,
T18382 6632-6636 NNP denotes Gzmb
T18383 6636-6637 , denotes ,
T18384 6638-6643 NNP denotes Tbx21
T18385 6644-6645 -LRB- denotes (
T18386 6645-6650 NNP denotes T-bet
T18387 6650-6651 -RRB- denotes )
T18388 6651-6652 , denotes ,
T18389 6653-6656 CC denotes and
T18390 6657-6662 NNP denotes Eomes
T18391 6663-6667 NNP denotes mRNA
T18392 6668-6678 NN denotes expression
T18393 6679-6681 IN denotes in
T18394 6682-6697 VBG denotes differentiating
T18395 6698-6701 NNP denotes P14
T18396 6702-6705 NNP denotes CD8
T18397 6705-6706 VBD denotes +
T18398 6707-6708 NNP denotes T
T18399 6709-6714 NNS denotes cells
T18400 6715-6723 VBD denotes analyzed
T18401 6724-6726 IN denotes by
T18402 6727-6735 NNP denotes Northern
T18403 6736-6744 VBG denotes blotting
T18404 6744-6745 . denotes .
T18405 6746-6749 NNP denotes RNA
T18406 6750-6754 IN denotes from
T18407 6755-6758 NN denotes day
T18408 6759-6760 CD denotes 7
T18409 6761-6764 CD denotes Th1
T18410 6765-6770 NNS denotes cells
T18411 6771-6774 VBD denotes was
T18412 6775-6779 VBN denotes used
T18413 6780-6782 IN denotes as
T18414 6783-6784 DT denotes a
T18415 6785-6792 NN denotes control
T18416 6792-6793 . denotes .
T18417 6794-6799 NNS denotes Sizes
T18418 6800-6802 IN denotes of
T18419 6803-6807 NNP denotes mRNA
T18420 6808-6819 NNS denotes transcripts
T18421 6820-6823 VBP denotes are
T18422 6824-6833 VBN denotes indicated
T18423 6833-6834 . denotes .
T18424 6835-6836 -LRB- denotes (
T18425 6836-6837 NNP denotes B
T18426 6837-6838 -RRB- denotes )
T18427 6839-6853 NN denotes Quantification
T18428 6854-6856 IN denotes of
T18429 6857-6865 JJ denotes relative
T18430 6866-6870 NNP denotes mRNA
T18431 6871-6878 NNS denotes amounts
T18432 6879-6881 IN denotes by
T18433 6882-6896 NN denotes phosphorimager
T18434 6897-6905 NN denotes analysis
T18435 6905-6906 . denotes .
T18436 6907-6908 -LRB- denotes (
T18437 6908-6909 $ denotes C
T18438 6909-6910 -RRB- denotes )
T18439 6911-6919 NNPS denotes Kinetics
T18440 6920-6922 IN denotes of
T18441 6923-6930 NN denotes protein
T18442 6931-6941 NN denotes expression
T18443 6942-6944 IN denotes in
T18444 6945-6960 VBG denotes differentiating
T18445 6961-6964 NNP denotes P14
T18446 6965-6968 NNP denotes CD8
T18447 6968-6969 VBD denotes +
T18448 6970-6971 NNP denotes T
T18449 6972-6977 NNS denotes cells
T18450 6978-6986 VBD denotes analyzed
T18451 6987-6989 IN denotes by
T18452 6990-7004 VBG denotes immunoblotting
T18453 7004-7005 . denotes .
T18454 7006-7011 NNS denotes Sizes
T18455 7012-7014 IN denotes of
T18456 7015-7022 NN denotes protein
T18457 7023-7028 NNS denotes bands
T18458 7029-7032 VBP denotes are
T18459 7033-7042 VBN denotes indicated
T18460 7042-7043 . denotes .
T18461 7044-7045 -LRB- denotes (
T18462 7045-7046 NNP denotes D
T18463 7046-7047 -RRB- denotes )
T18464 7048-7056 JJ denotes Relative
T18465 7057-7064 NN denotes protein
T18466 7065-7072 NNS denotes amounts
T18467 7073-7083 VBN denotes quantified
T18468 7084-7088 IN denotes from
T18469 7089-7092 DT denotes the
T18470 7093-7100 JJ denotes Western
T18471 7101-7106 NNS denotes blots
T18472 7106-7107 . denotes .
T18473 7108-7109 -LRB- denotes (
T18474 7109-7110 NNP denotes E
T18475 7110-7111 -RRB- denotes )
T18476 7112-7125 NNP denotes Intracellular
T18477 7126-7134 VBG denotes staining
T18478 7135-7138 IN denotes for
T18479 7139-7147 NN denotes granzyme
T18480 7148-7149 NNP denotes B
T18481 7149-7150 , denotes ,
T18482 7151-7156 NNP denotes IFN-γ
T18483 7156-7157 , denotes ,
T18484 7158-7161 CC denotes and
T18485 7162-7165 NNP denotes TNF
T18486 7165-7166 . denotes .
T18487 7167-7175 NNP denotes Granzyme
T18488 7176-7177 NNP denotes B
T18489 7178-7186 VBG denotes staining
T18490 7187-7190 VBD denotes was
T18491 7191-7199 JJ denotes specific
T18492 7200-7208 JJ denotes relative
T18493 7209-7211 TO denotes to
T18494 7212-7214 DT denotes an
T18495 7215-7222 NN denotes isotype
T18496 7223-7230 NN denotes control
T18497 7231-7232 -LRB- denotes (
T18498 7232-7235 RB denotes not
T18499 7236-7244 VBN denotes depicted
T18500 7244-7245 -RRB- denotes )
T18501 7245-7246 . denotes .
T18502 7247-7252 NNS denotes Cells
T18503 7253-7257 VBD denotes were
T18504 7258-7270 VBN denotes restimulated
T18505 7271-7275 IN denotes with
T18506 7276-7279 NNP denotes PMA
T18507 7280-7283 CC denotes and
T18508 7284-7293 NN denotes ionomycin
T18509 7294-7297 IN denotes for
T18510 7298-7299 CD denotes 4
T18511 7300-7302 NN denotes h.
T18512 7303-7304 -LRB- denotes (
T18513 7304-7305 NN denotes F
T18514 7305-7306 -RRB- denotes )
T18515 7307-7317 JJ denotes FACS-based
T18516 7318-7323 NN denotes assay
T18517 7324-7326 TO denotes to
T18518 7327-7334 VB denotes measure
T18519 7335-7344 JJ denotes cytolytic
T18520 7345-7353 NN denotes activity
T18521 7354-7356 IN denotes of
T18522 7357-7360 NNP denotes P14
T18523 7361-7364 NNP denotes CD8
T18524 7364-7365 VBD denotes +
T18525 7366-7367 NNP denotes T
T18526 7368-7373 NNS denotes cells
T18527 7374-7381 IN denotes against
T18528 7382-7385 CD denotes EL4
T18529 7386-7393 NNS denotes targets
T18530 7394-7400 VBN denotes loaded
T18531 7401-7405 IN denotes with
T18532 7406-7407 CD denotes 0
T18533 7408-7409 -LRB- denotes (
T18534 7409-7410 NN denotes −
T18535 7410-7411 -RRB- denotes )
T18536 7412-7414 CC denotes or
T18537 7415-7416 CD denotes 1
T18538 7417-7418 -LRB- denotes (
T18539 7418-7419 NN denotes +
T18540 7419-7420 -RRB- denotes )
T18541 7421-7423 NNP denotes μM
T18542 7424-7428 NNP denotes Gp33
T18543 7429-7436 NN denotes peptide
T18544 7437-7438 -LRB- denotes (
T18545 7438-7456 JJ denotes effector-to-target
T18546 7457-7462 NN denotes ratio
T18547 7463-7464 SYM denotes =
T18548 7465-7468 CD denotes 5:1
T18549 7468-7469 -RRB- denotes )
T18550 7469-7470 . denotes .
T18551 7471-7481 NN denotes Percentage
T18552 7482-7484 IN denotes of
T18553 7485-7492 NNP denotes Annexin
T18554 7493-7494 NNP denotes V
T18555 7494-7495 NN denotes +
T18556 7496-7497 -LRB- denotes (
T18557 7497-7506 JJ denotes apoptotic
T18558 7506-7507 -RRB- denotes )
T18559 7508-7514 NN denotes target
T18560 7515-7520 NNS denotes cells
T18561 7521-7523 IN denotes in
T18562 7524-7527 DT denotes the
T18563 7528-7540 JJ denotes CD8-negative
T18564 7541-7544 CD denotes EL4
T18565 7545-7551 NN denotes target
T18566 7552-7562 NN denotes population
T18567 7563-7564 -LRB- denotes (
T18568 7564-7567 VB denotes dot
T18569 7568-7573 NNS denotes plots
T18570 7573-7574 -RRB- denotes )
T18571 7575-7578 VBD denotes was
T18572 7579-7589 VBN denotes determined
T18573 7590-7591 -LRB- denotes (
T18574 7591-7601 NNS denotes histograms
T18575 7601-7602 -RRB- denotes )
T18576 7602-7603 . denotes .
T18577 7604-7613 JJ denotes Cytolytic
T18578 7614-7622 NN denotes activity
T18579 7623-7626 VBD denotes was
T18580 7627-7634 VBN denotes blocked
T18581 7635-7637 IN denotes by
T18582 7638-7648 NN denotes incubation
T18583 7649-7653 IN denotes with
T18584 7654-7655 CD denotes 2
T18585 7656-7658 NNP denotes mM
T18586 7659-7663 NNP denotes EGTA
T18587 7664-7665 -LRB- denotes (
T18588 7665-7668 RB denotes not
T18589 7669-7677 VBN denotes depicted
T18590 7677-7678 -RRB- denotes )
T18591 7678-7679 , denotes ,
T18592 7680-7690 VBG denotes confirming
T18593 7691-7702 NN denotes involvement
T18594 7703-7705 IN denotes of
T18595 7706-7709 DT denotes the
T18596 7710-7717 NN denotes granule
T18597 7718-7728 NN denotes exocytosis
T18598 7729-7730 -LRB- denotes (
T18599 7730-7738 NN denotes perforin
T18600 7739-7747 NN denotes granzyme
T18601 7748-7749 NN denotes B
T18602 7749-7750 -RRB- denotes )
T18603 7751-7758 NN denotes pathway
T18604 7758-7759 . denotes .
T18605 7760-7764 NNS denotes Data
T18606 7765-7768 VBP denotes are
T18607 7769-7783 NN denotes representative
T18608 7784-7786 IN denotes of
T18609 7787-7789 IN denotes at
T18610 7790-7795 JJS denotes least
T18611 7796-7800 CD denotes five
T18612 7801-7802 -LRB- denotes (
T18613 7802-7803 DT denotes A
T18614 7804-7805 NNP denotes E
T18615 7805-7806 -RRB- denotes )
T18616 7807-7809 CC denotes or
T18617 7810-7815 CD denotes three
T18618 7816-7817 -LRB- denotes (
T18619 7817-7818 NN denotes F
T18620 7818-7819 -RRB- denotes )
T18621 7820-7831 JJ denotes independent
T18622 7832-7843 NNS denotes experiments
T18623 7843-7844 . denotes .
T19207 11884-11894 NN denotes Regulation
T19208 11895-11897 IN denotes of
T19209 11898-11906 NN denotes perforin
T19210 11906-11907 , denotes ,
T19211 11908-11916 NN denotes granzyme
T19212 11917-11918 NNP denotes B
T19213 11918-11919 , denotes ,
T19214 11920-11923 CC denotes and
T19215 11924-11929 NNP denotes IFN-γ
T19216 11930-11940 NN denotes expression
T19217 11941-11943 IN denotes by
T19218 11944-11949 NNP denotes T-bet
T19219 11950-11953 CC denotes and
T19220 11954-11959 NNP denotes Eomes
T19221 11960-11962 IN denotes in
T19222 11963-11978 VBG denotes differentiating
T19223 11979-11983 NNS denotes CTLs
T19224 11983-11984 . denotes .
T19225 11985-11986 -LRB- denotes (
T19226 11986-11987 NNP denotes A
T19227 11987-11988 -RRB- denotes )
T19228 11989-11994 JJ denotes IFN-γ
T19229 11995-12005 NN denotes expression
T19230 12006-12008 IN denotes by
T19231 12009-12011 NNP denotes WT
T19232 12012-12013 -LRB- denotes (
T19233 12013-12018 NNP denotes Tbx21
T19234 12018-12019 CD denotes +
T19235 12019-12020 CD denotes /
T19236 12020-12021 NN denotes +
T19237 12021-12022 -RRB- denotes )
T19238 12023-12026 CC denotes and
T19239 12027-12032 NNP denotes T-bet
T19240 12033-12042 NN denotes deficient
T19241 12043-12044 -LRB- denotes (
T19242 12044-12049 CD denotes Tbx21
T19243 12049-12050 CD denotes −
T19244 12050-12051 NN denotes /
T19245 12051-12052 NN denotes −
T19246 12052-12053 -RRB- denotes )
T19247 12054-12055 NN denotes T
T19248 12056-12061 NNS denotes cells
T19249 12061-12062 . denotes .
T19250 12063-12068 JJ denotes Naive
T19251 12069-12072 NNP denotes CD8
T19252 12072-12073 CD denotes +
T19253 12074-12075 NNP denotes T
T19254 12076-12081 NNS denotes cells
T19255 12081-12082 , denotes ,
T19256 12083-12085 CC denotes or
T19257 12086-12091 NNS denotes cells
T19258 12092-12101 VBN denotes activated
T19259 12102-12105 CC denotes and
T19260 12106-12114 VBN denotes cultured
T19261 12115-12118 IN denotes for
T19262 12119-12120 CD denotes 4
T19263 12121-12123 CC denotes or
T19264 12124-12125 CD denotes 6
T19265 12126-12127 LS denotes d
T19266 12127-12128 , denotes ,
T19267 12129-12133 VBD denotes were
T19268 12134-12146 VBN denotes restimulated
T19269 12147-12151 IN denotes with
T19270 12152-12155 NNP denotes PMA
T19271 12156-12159 CC denotes and
T19272 12160-12169 NN denotes ionomycin
T19273 12170-12173 IN denotes for
T19274 12174-12175 CD denotes 6
T19275 12176-12177 NN denotes h
T19276 12177-12178 , denotes ,
T19277 12179-12182 CC denotes and
T19278 12183-12188 NNP denotes IFN-γ
T19279 12189-12199 NN denotes expression
T19280 12200-12203 VBD denotes was
T19281 12204-12212 VBN denotes assessed
T19282 12213-12215 IN denotes by
T19283 12216-12229 JJ denotes intracellular
T19284 12230-12238 NN denotes staining
T19285 12238-12239 . denotes .
T19286 12240-12247 NNS denotes Numbers
T19287 12248-12252 VBP denotes show
T19288 12253-12256 DT denotes the
T19289 12257-12267 NN denotes percentage
T19290 12268-12270 IN denotes of
T19291 12271-12276 JJ denotes IFN-γ
T19292 12276-12277 NN denotes +
T19293 12278-12283 NNS denotes cells
T19294 12283-12284 . denotes .
T19295 12285-12286 -LRB- denotes (
T19296 12286-12287 NNP denotes B
T19297 12287-12288 -RRB- denotes )
T19298 12289-12297 NNP denotes Northern
T19299 12298-12302 NN denotes blot
T19300 12303-12311 NN denotes analysis
T19301 12312-12314 IN denotes of
T19302 12315-12319 NNP denotes Prf1
T19303 12320-12323 CC denotes and
T19304 12324-12328 NNP denotes GzmB
T19305 12329-12333 NNP denotes mRNA
T19306 12334-12344 NN denotes expression
T19307 12345-12347 IN denotes in
T19308 12348-12350 NNP denotes WT
T19309 12351-12353 CC denotes or
T19310 12354-12359 NNP denotes T-bet
T19311 12360-12369 JJ denotes deficient
T19312 12370-12373 NNP denotes CD8
T19313 12373-12374 CD denotes +
T19314 12375-12376 NNP denotes T
T19315 12377-12382 NNS denotes cells
T19316 12383-12392 VBN denotes activated
T19317 12393-12396 CC denotes and
T19318 12397-12403 RB denotes either
T19319 12404-12408 VBD denotes left
T19320 12409-12419 JJ denotes uninfected
T19321 12420-12421 -LRB- denotes (
T19322 12421-12426 JJ denotes uninf
T19323 12426-12427 -RRB- denotes )
T19324 12428-12430 CC denotes or
T19325 12431-12441 VBN denotes transduced
T19326 12442-12446 IN denotes with
T19327 12447-12459 NNS denotes retroviruses
T19328 12460-12470 VBG denotes expressing
T19329 12471-12481 NNP denotes Eomes-VP16
T19330 12482-12483 -LRB- denotes (
T19331 12483-12490 NNP denotes Eo-VP16
T19332 12490-12491 -RRB- denotes )
T19333 12492-12494 CC denotes or
T19334 12495-12497 DT denotes an
T19335 12498-12503 JJ denotes empty
T19336 12504-12512 NNP denotes IRES-GFP
T19337 12513-12521 NN denotes cassette
T19338 12522-12523 -LRB- denotes (
T19339 12523-12526 NNP denotes GFP
T19340 12526-12527 -RRB- denotes )
T19341 12527-12528 . denotes .
T19342 12529-12534 JJ denotes Total
T19343 12535-12543 JJ denotes cellular
T19344 12544-12547 NNP denotes RNA
T19345 12548-12551 VBD denotes was
T19346 12552-12560 VBN denotes analyzed
T19347 12561-12563 IN denotes on
T19348 12564-12567 NN denotes day
T19349 12568-12569 CD denotes 6
T19350 12570-12572 IN denotes of
T19351 12573-12580 NN denotes culture
T19352 12580-12581 . denotes .
T19353 12582-12585 DT denotes The
T19354 12586-12595 NN denotes frequency
T19355 12596-12598 IN denotes of
T19356 12599-12609 JJ denotes transduced
T19357 12610-12615 NNS denotes cells
T19358 12616-12618 IN denotes in
T19359 12619-12622 DT denotes the
T19360 12623-12631 NNS denotes cultures
T19361 12632-12635 VBD denotes was
T19362 12636-12646 JJ denotes equivalent
T19363 12647-12650 IN denotes for
T19364 12651-12655 DT denotes both
T19365 12656-12666 NNS denotes constructs
T19366 12667-12668 -LRB- denotes (
T19367 12668-12669 CD denotes ∼
T19368 12669-12671 CD denotes 65
T19369 12672-12674 CD denotes 70
T19370 12674-12675 NN denotes %
T19371 12676-12679 NNP denotes GFP
T19372 12679-12680 CD denotes +
T19373 12681-12686 NNS denotes cells
T19374 12686-12687 : denotes ;
T19375 12688-12691 RB denotes not
T19376 12692-12700 VBN denotes depicted
T19377 12700-12701 -RRB- denotes )
T19378 12701-12702 . denotes .
T19379 12703-12704 -LRB- denotes (
T19380 12704-12705 NNP denotes C
T19381 12705-12706 -RRB- denotes )
T19382 12707-12715 NNP denotes Granzyme
T19383 12716-12717 NNP denotes B
T19384 12718-12721 CC denotes and
T19385 12722-12727 NNP denotes IFN-γ
T19386 12728-12738 NN denotes expression
T19387 12739-12741 IN denotes by
T19388 12742-12747 NNP denotes Tbx21
T19389 12747-12748 NNP denotes +
T19390 12748-12749 VBD denotes /
T19391 12749-12750 CD denotes +
T19392 12751-12754 CC denotes and
T19393 12755-12760 CD denotes Tbx21
T19394 12760-12761 CD denotes −
T19395 12761-12762 NN denotes /
T19396 12762-12763 CD denotes −
T19397 12764-12765 NNP denotes T
T19398 12766-12771 NNS denotes cells
T19399 12772-12780 VBD denotes analyzed
T19400 12781-12783 IN denotes in
T19401 12784-12796 JJ denotes restimulated
T19402 12797-12802 NNS denotes cells
T19403 12803-12807 WDT denotes that
T19404 12808-12811 VBD denotes had
T19405 12812-12816 VBN denotes been
T19406 12817-12825 VBN denotes cultured
T19407 12826-12829 IN denotes for
T19408 12830-12831 CD denotes 5
T19409 12832-12834 NN denotes d.
T19410 12835-12836 -LRB- denotes (
T19411 12836-12837 NNP denotes D
T19412 12837-12838 -RRB- denotes )
T19413 12839-12844 JJ denotes IFN-γ
T19414 12845-12855 NN denotes production
T19415 12856-12858 IN denotes by
T19416 12859-12864 NNS denotes cells
T19417 12865-12875 VBN denotes transduced
T19418 12876-12880 IN denotes with
T19419 12881-12888 NN denotes Eo-VP16
T19420 12889-12891 CC denotes or
T19421 12892-12899 NN denotes control
T19422 12900-12901 -LRB- denotes (
T19423 12901-12904 NNP denotes GFP
T19424 12904-12905 -RRB- denotes )
T19425 12906-12918 NNS denotes retroviruses
T19426 12919-12920 -LRB- denotes (
T19427 12920-12922 NN denotes RV
T19428 12922-12923 -RRB- denotes )
T19429 12924-12932 VBN denotes measured
T19430 12933-12935 IN denotes on
T19431 12936-12939 NN denotes day
T19432 12940-12941 CD denotes 4
T19433 12942-12947 IN denotes after
T19434 12948-12949 CD denotes 6
T19435 12950-12951 NN denotes h
T19436 12952-12954 IN denotes of
T19437 12955-12968 NN denotes restimulation
T19438 12969-12973 IN denotes with
T19439 12974-12977 NNP denotes PMA
T19440 12978-12981 CC denotes and
T19441 12982-12991 NN denotes ionomycin
T19442 12991-12992 . denotes .
T19443 12993-13000 NNS denotes Numbers
T19444 13001-13005 VBP denotes show
T19445 13006-13009 DT denotes the
T19446 13010-13020 NN denotes percentage
T19447 13021-13023 IN denotes of
T19448 13024-13027 NNP denotes GFP
T19449 13027-13028 VBP denotes +
T19450 13029-13034 JJ denotes IFN-γ
T19451 13034-13035 NN denotes +
T19452 13036-13041 NNS denotes cells
T19453 13041-13042 . denotes .
T19454 13043-13050 NNS denotes Results
T19455 13051-13054 VBP denotes are
T19456 13055-13069 NN denotes representative
T19457 13070-13072 IN denotes of
T19458 13073-13078 CD denotes three
T19459 13079-13080 -LRB- denotes (
T19460 13080-13081 DT denotes A
T19461 13082-13085 CC denotes and
T19462 13086-13087 NNP denotes C
T19463 13087-13088 -RRB- denotes )
T19464 13089-13091 CC denotes or
T19465 13092-13095 CD denotes two
T19466 13096-13097 -LRB- denotes (
T19467 13097-13098 NN denotes B
T19468 13099-13102 CC denotes and
T19469 13103-13104 NNP denotes D
T19470 13104-13105 -RRB- denotes )
T19471 13106-13117 JJ denotes independent
T19472 13118-13129 NNS denotes experiments
T19473 13129-13130 . denotes .
T20185 15058-15061 NNP denotes Key
T20186 15062-15066 NN denotes role
T20187 15067-15070 IN denotes for
T20188 15071-15076 NNP denotes Runx3
T20189 15077-15079 IN denotes in
T20190 15080-15088 NN denotes effector
T20191 15089-15092 NNP denotes CTL
T20192 15093-15108 NN denotes differentiation
T20193 15108-15109 . denotes .
T20194 15110-15111 -LRB- denotes (
T20195 15111-15112 NNP denotes A
T20196 15112-15113 -RRB- denotes )
T20197 15114-15121 NNP denotes Western
T20198 15122-15130 NN denotes analysis
T20199 15131-15133 IN denotes of
T20200 15134-15139 NNP denotes Runx3
T20201 15139-15140 , denotes ,
T20202 15141-15146 NNP denotes Eomes
T20203 15146-15147 , denotes ,
T20204 15148-15153 NNP denotes T-bet
T20205 15153-15154 , denotes ,
T20206 15155-15158 CC denotes and
T20207 15159-15167 NN denotes perforin
T20208 15168-15178 NN denotes expression
T20209 15179-15181 IN denotes in
T20210 15182-15187 NNP denotes Runx3
T20211 15187-15188 NNP denotes +
T20212 15188-15189 VBD denotes /
T20213 15189-15190 CD denotes +
T20214 15191-15197 CC denotes versus
T20215 15198-15203 NNP denotes Runx3
T20216 15203-15204 FW denotes −
T20217 15204-15205 FW denotes /
T20218 15205-15206 FW denotes −
T20219 15207-15210 FW denotes CD8
T20220 15210-15211 FW denotes +
T20221 15212-15214 FW denotes SP
T20222 15215-15216 FW denotes T
T20223 15217-15222 NNS denotes cells
T20224 15223-15237 VBN denotes differentiated
T20225 15238-15241 IN denotes for
T20226 15242-15243 CD denotes 6
T20227 15244-15246 NN denotes d.
T20228 15247-15254 NN denotes β-Actin
T20229 15255-15258 VBD denotes was
T20230 15259-15263 VBN denotes used
T20231 15264-15266 IN denotes as
T20232 15267-15268 DT denotes a
T20233 15269-15276 VBG denotes loading
T20234 15277-15284 NN denotes control
T20235 15284-15285 . denotes .
T20236 15286-15287 -LRB- denotes (
T20237 15287-15288 NNP denotes B
T20238 15288-15289 -RRB- denotes )
T20239 15290-15298 NNP denotes Northern
T20240 15299-15303 NN denotes blot
T20241 15304-15312 NN denotes analysis
T20242 15313-15315 IN denotes of
T20243 15316-15320 NNP denotes Prf1
T20244 15321-15325 NNP denotes mRNA
T20245 15326-15336 NN denotes expression
T20246 15337-15339 IN denotes in
T20247 15340-15345 NNP denotes Runx3
T20248 15345-15346 NNP denotes +
T20249 15346-15347 VBD denotes /
T20250 15347-15348 CD denotes +
T20251 15349-15355 CC denotes versus
T20252 15356-15361 NNP denotes Runx3
T20253 15361-15362 FW denotes −
T20254 15362-15363 FW denotes /
T20255 15363-15364 FW denotes −
T20256 15365-15368 FW denotes CD8
T20257 15368-15369 FW denotes +
T20258 15370-15371 FW denotes T
T20259 15372-15377 NNS denotes cells
T20260 15378-15392 VBN denotes differentiated
T20261 15393-15396 IN denotes for
T20262 15397-15398 CD denotes 6
T20263 15399-15401 NN denotes d.
T20264 15402-15403 NN denotes β
T20265 15404-15409 NNP denotes Actin
T20266 15410-15413 VBD denotes was
T20267 15414-15418 VBN denotes used
T20268 15419-15421 IN denotes as
T20269 15422-15423 DT denotes a
T20270 15424-15431 VBG denotes loading
T20271 15432-15439 NN denotes control
T20272 15439-15440 . denotes .
T20273 15441-15442 -LRB- denotes (
T20274 15442-15443 $ denotes C
T20275 15443-15444 -RRB- denotes )
T20276 15445-15455 NN denotes Expression
T20277 15456-15458 IN denotes of
T20278 15459-15467 NN denotes granzyme
T20279 15468-15469 NNP denotes B
T20280 15469-15470 , denotes ,
T20281 15471-15476 NNP denotes IFN-γ
T20282 15476-15477 , denotes ,
T20283 15478-15481 NNP denotes TNF
T20284 15481-15482 , denotes ,
T20285 15483-15486 CC denotes and
T20286 15487-15491 NN denotes IL-2
T20287 15492-15494 IN denotes by
T20288 15495-15502 VBG denotes resting
T20289 15503-15505 CC denotes or
T20290 15506-15518 VBN denotes restimulated
T20291 15519-15520 -LRB- denotes (
T20292 15520-15521 CD denotes 6
T20293 15522-15523 NN denotes h
T20294 15523-15524 -RRB- denotes )
T20295 15525-15530 JJ denotes Runx3
T20296 15530-15531 NN denotes +
T20297 15531-15532 NN denotes /
T20298 15532-15533 NN denotes +
T20299 15534-15540 IN denotes versus
T20300 15541-15546 NNP denotes Runx3
T20301 15546-15547 NNP denotes −
T20302 15547-15548 VBD denotes /
T20303 15548-15549 CD denotes −
T20304 15550-15553 CD denotes CD8
T20305 15553-15554 NN denotes +
T20306 15555-15557 NNP denotes SP
T20307 15558-15559 NNP denotes T
T20308 15560-15565 NNS denotes cells
T20309 15566-15580 VBD denotes differentiated
T20310 15581-15584 IN denotes for
T20311 15585-15586 CD denotes 6
T20312 15587-15588 LS denotes d
T20313 15588-15589 . denotes .
T20314 15590-15593 DT denotes The
T20315 15594-15602 JJ denotes vertical
T20316 15603-15607 JJ denotes gray
T20317 15608-15612 NN denotes line
T20318 15613-15622 VBZ denotes indicates
T20319 15623-15626 DT denotes the
T20320 15627-15635 NN denotes granzyme
T20321 15636-15637 NNP denotes B
T20322 15638-15641 NNP denotes MFI
T20323 15642-15645 IN denotes for
T20324 15646-15648 NNP denotes WT
T20325 15649-15652 NNP denotes GFP
T20326 15652-15653 NN denotes +
T20327 15654-15659 NNS denotes cells
T20328 15659-15660 . denotes .
T20329 15661-15668 NNS denotes Results
T20330 15669-15671 IN denotes in
T20331 15672-15673 DT denotes A
T20332 15674-15675 NNP denotes C
T20333 15676-15679 VBP denotes are
T20334 15680-15694 JJ denotes representative
T20335 15695-15697 IN denotes of
T20336 15698-15701 CD denotes two
T20337 15702-15713 JJ denotes independent
T20338 15714-15725 NNS denotes experiments
T20339 15725-15726 . denotes .
T20340 15727-15728 -LRB- denotes (
T20341 15728-15729 NNP denotes D
T20342 15729-15730 -RRB- denotes )
T20343 15731-15735 NNP denotes ChIP
T20344 15736-15744 NN denotes analysis
T20345 15745-15747 IN denotes of
T20346 15748-15755 JJ denotes binding
T20347 15756-15758 IN denotes of
T20348 15759-15769 JJ denotes endogenous
T20349 15770-15775 NNP denotes Runx3
T20350 15776-15779 CC denotes and
T20351 15780-15785 NNP denotes Eomes
T20352 15786-15788 TO denotes to
T20353 15789-15792 DT denotes the
T20354 15793-15797 JJ denotes Prf1
T20355 15798-15803 NN denotes locus
T20356 15803-15804 . denotes .
T20357 15805-15815 NNP denotes Enrichment
T20358 15816-15818 IN denotes of
T20393 16039-16040 NNP denotes T
T20394 16041-16045 NN denotes cell
T20395 16046-16062 NNS denotes differentiations
T20396 16062-16063 . denotes .
T20397 16064-16067 DT denotes The
T20398 16068-16078 NN denotes efficiency
T20399 16079-16081 IN denotes of
T20400 16082-16090 NN denotes recovery
T20401 16091-16093 IN denotes of
T20402 16094-16099 NN denotes input
T20403 16100-16103 IN denotes for
T20404 16104-16107 DT denotes the
T20405 16108-16109 NN denotes −
T20406 16109-16113 JJ denotes 1-kb
T20407 16114-16120 NN denotes region
T20408 16121-16123 IN denotes of
T20409 16124-16128 NNP denotes Prf1
T20410 16129-16132 VBD denotes was
T20411 16133-16137 CD denotes 0.97
T20412 16137-16138 NN denotes %
T20413 16139-16142 IN denotes for
T20414 16143-16146 DT denotes the
T20415 16147-16152 NNP denotes Runx3
T20416 16153-16157 NNP denotes ChIP
T20417 16158-16161 CC denotes and
T20418 16162-16165 CD denotes 0.5
T20419 16165-16166 NN denotes %
T20420 16167-16170 IN denotes for
T20421 16171-16174 DT denotes the
T20422 16175-16180 NNP denotes Eomes
T20423 16181-16185 NNP denotes ChIP
T20424 16185-16186 . denotes .
T20970 19226-19227 , denotes ,
T20971 19228-19236 NN denotes perforin
T20972 19236-19237 , denotes ,
T20973 19238-19246 NN denotes granzyme
T20974 19247-19248 NNP denotes B
T20975 19248-19249 , denotes ,
T20976 19250-19253 CC denotes and
T20977 19254-19259 NNP denotes IFN-γ
T20978 19260-19270 NN denotes expression
T20979 19271-19273 IN denotes in
T20980 19274-19282 NN denotes effector
T20981 19283-19287 NNS denotes CTLs
T20982 19287-19288 . denotes .
T20983 19289-19294 JJ denotes Runx3
T20984 19294-19295 NN denotes +
T20985 19295-19296 NN denotes /
T20986 19296-19297 NN denotes +
T20987 19298-19300 CC denotes or
T20988 19301-19306 JJ denotes Runx3
T20989 19306-19307 FW denotes −
T20990 19307-19308 FW denotes /
T20991 19308-19309 FW denotes −
T20992 19310-19313 FW denotes CD8
T20993 19313-19314 FW denotes +
T20994 19315-19316 FW denotes T
T20995 19317-19322 NNS denotes cells
T20996 19323-19327 VBD denotes were
T20997 19328-19337 VBN denotes activated
T20998 19338-19341 CC denotes and
T20999 19342-19352 VBN denotes transduced
T21000 19353-19357 IN denotes with
T21001 19358-19370 NNS denotes retroviruses
T21002 19371-19378 VBG denotes bearing
T21003 19379-19381 DT denotes an
T21004 19382-19387 JJ denotes empty
T21005 19388-19396 NNP denotes IRES-GFP
T21006 19397-19405 NN denotes cassette
T21007 19406-19407 -LRB- denotes (
T21008 19407-19410 NNP denotes GFP
T21009 19410-19411 -RRB- denotes )
T21010 19412-19414 CC denotes or
T21011 19415-19419 RB denotes also
T21012 19420-19428 VBG denotes encoding
T21013 19429-19439 NNP denotes Eomes-VP16
T21014 19440-19441 -LRB- denotes (
T21015 19441-19448 NNP denotes Eo-VP16
T21016 19448-19449 -RRB- denotes )
T21017 19450-19452 CC denotes or
T21018 19453-19462 JJ denotes Myc-Runx3
T21019 19463-19464 -LRB- denotes (
T21020 19464-19469 JJ denotes Runx3
T21021 19469-19470 -RRB- denotes )
T21022 19470-19471 . denotes .
T21023 19472-19475 DT denotes The
T21024 19476-19485 NN denotes frequency
T21025 19486-19488 IN denotes of
T21026 19489-19499 JJ denotes transduced
T21027 19500-19505 NNS denotes cells
T21028 19506-19508 IN denotes in
T21029 19509-19512 DT denotes the
T21030 19513-19521 NNS denotes cultures
T21031 19522-19525 VBD denotes was
T21032 19526-19536 JJ denotes equivalent
T21033 19537-19540 IN denotes for
T21034 19541-19544 DT denotes all
T21035 19545-19555 NNS denotes constructs
T21036 19556-19557 -LRB- denotes (
T21037 19557-19558 CD denotes ∼
T21038 19558-19560 CD denotes 75
T21039 19561-19563 CD denotes 90
T21040 19563-19564 NN denotes %
T21041 19565-19568 NNP denotes GFP
T21042 19568-19569 CD denotes +
T21043 19570-19575 NNS denotes cells
T21044 19575-19576 : denotes ;
T21045 19577-19580 RB denotes not
T21046 19581-19589 VBN denotes depicted
T21047 19589-19590 -RRB- denotes )
T21048 19590-19591 . denotes .
T21049 19592-19593 -LRB- denotes (
T21050 19593-19594 NNP denotes A
T21051 19594-19595 -RRB- denotes )
T21052 19596-19603 NNP denotes Protein
T21053 19604-19614 NN denotes expression
T21054 19615-19617 IN denotes in
T21055 19618-19628 JJ denotes whole-cell
T21056 19629-19637 NNS denotes extracts
T21057 19638-19639 -LRB- denotes (
T21058 19639-19642 NN denotes day
T21059 19643-19644 CD denotes 6
T21060 19644-19645 -RRB- denotes )
T21061 19646-19649 VBD denotes was
T21062 19650-19658 VBN denotes analyzed
T21063 19659-19661 IN denotes by
T21064 19662-19676 VBG denotes immunoblotting
T21065 19676-19677 . denotes .
T21066 19678-19692 NNP denotes Overexpression
T21067 19693-19695 IN denotes of
T21068 19696-19706 NNP denotes Eomes-VP16
T21069 19707-19710 MD denotes can
T21070 19710-19713 RB denotes not
T21071 19714-19716 VB denotes be
T21072 19717-19725 VBN denotes detected
T21073 19726-19730 IN denotes with
T21074 19731-19734 DT denotes the
T21075 19735-19740 NNP denotes Eomes
T21076 19741-19749 NN denotes antibody
T21077 19749-19750 , denotes ,
T21078 19751-19753 IN denotes as
T21079 19754-19757 DT denotes the
T21080 19758-19768 JJ denotes C-terminal
T21081 19769-19776 NN denotes epitope
T21082 19777-19779 VBZ denotes is
T21083 19780-19786 IN denotes within
T21084 19787-19790 DT denotes the
T21085 19791-19797 NN denotes region
T21086 19798-19802 WDT denotes that
T21087 19803-19806 VBZ denotes has
T21088 19807-19811 VBN denotes been
T21089 19812-19820 VBN denotes replaced
T21090 19821-19825 IN denotes with
T21091 19826-19829 DT denotes the
T21092 19830-19834 NNP denotes VP16
T21093 19835-19850 NN denotes transactivation
T21094 19851-19857 NN denotes domain
T21095 19857-19858 . denotes .
T21096 19859-19860 -LRB- denotes (
T21097 19860-19861 NNP denotes B
T21098 19861-19862 -RRB- denotes )
T21099 19863-19873 NN denotes Expression
T21100 19874-19876 IN denotes of
T21101 19877-19885 NN denotes granzyme
T21102 19886-19887 NNP denotes B
T21103 19888-19891 CC denotes and
T21104 19892-19897 NNP denotes IFN-γ
T21105 19898-19903 IN denotes after
T21106 19904-19911 NN denotes culture
T21107 19912-19915 IN denotes for
T21108 19916-19917 CD denotes 6
T21109 19918-19919 LS denotes d
T21110 19920-19923 CC denotes and
T21111 19924-19937 NN denotes restimulation
T21112 19938-19941 IN denotes for
T21113 19942-19943 CD denotes 4
T21114 19944-19945 NN denotes h
T21115 19946-19950 IN denotes with
T21116 19951-19954 NNP denotes PMA
T21117 19955-19958 CC denotes and
T21118 19959-19968 NN denotes ionomycin
T21119 19969-19972 VBD denotes was
T21120 19973-19983 VBN denotes determined
T21121 19984-19986 IN denotes by
T21122 19987-20000 JJ denotes intracellular
T21123 20001-20009 NN denotes staining
T21124 20009-20010 . denotes .
T21125 20011-20014 DT denotes The
T21126 20015-20025 NN denotes percentage
T21127 20026-20028 IN denotes of
T21128 20029-20039 RB denotes positively
T21129 20040-20047 VBN denotes stained
T21130 20048-20053 NNS denotes cells
T21131 20054-20056 VBZ denotes is
T21132 20057-20062 VBN denotes shown
T21133 20063-20068 IN denotes above
T21134 20069-20072 DT denotes the
T21135 20073-20077 NN denotes gate
T21136 20077-20078 : denotes ;
T21137 20079-20082 DT denotes the
T21138 20083-20087 JJ denotes mean
T21139 20088-20100 NN denotes fluorescence
T21140 20101-20110 NN denotes intensity
T21141 20111-20112 -LRB- denotes (
T21142 20112-20115 NNP denotes MFI
T21143 20115-20116 -RRB- denotes )
T21144 20117-20119 IN denotes of
T21145 20120-20128 NN denotes granzyme
T21146 20129-20130 NNP denotes B
T21147 20131-20139 VBG denotes staining
T21148 20140-20143 IN denotes for
T21149 20144-20147 DT denotes the
T21150 20148-20153 JJ denotes total
T21151 20154-20164 NN denotes population
T21152 20165-20167 VBZ denotes is
T21153 20168-20173 VBN denotes shown
T21154 20174-20179 IN denotes below
T21155 20180-20183 DT denotes the
T21156 20184-20188 NN denotes gate
T21157 20188-20189 . denotes .
T21158 20190-20193 DT denotes The
T21159 20194-20202 JJ denotes vertical
T21160 20203-20207 JJ denotes gray
T21161 20208-20213 NNS denotes lines
T21162 20214-20222 VBP denotes indicate
T21163 20223-20226 DT denotes the
T21164 20227-20230 NNP denotes MFI
T21165 20231-20234 IN denotes for
T21166 20235-20237 NNP denotes WT
T21167 20238-20241 NNP denotes GFP
T21168 20241-20242 NN denotes +
T21169 20243-20248 NNS denotes cells
T21170 20248-20249 . denotes .
T21171 20250-20257 NNS denotes Results
T21172 20258-20261 VBP denotes are
T21173 20262-20276 NN denotes representative
T21174 20277-20279 IN denotes of
T21175 20280-20282 IN denotes at
T21176 20283-20288 JJS denotes least
T21177 20289-20292 CD denotes two
T21178 20293-20304 JJ denotes independent
T21179 20305-20316 NNS denotes experiments
T21180 20316-20317 . denotes .
T21181 20318-20319 -LRB- denotes (
T21182 20319-20320 $ denotes C
T21183 20320-20321 -RRB- denotes )
T21184 20322-20331 JJ denotes Schematic
T21185 20332-20339 NN denotes diagram
T21186 20340-20342 IN denotes of
T21187 20343-20346 DT denotes the
T21188 20347-20362 JJ denotes transcriptional
T21189 20363-20370 NN denotes network
T21190 20371-20380 VBG denotes involving
T21191 20381-20386 NNP denotes Runx3
T21192 20387-20390 CC denotes and
T21193 20391-20396 NNP denotes T-box
T21194 20397-20404 NNS denotes factors
T21195 20404-20405 . denotes .
T21196 20406-20411 NN denotes T-bet
T21197 20412-20414 VBZ denotes is
T21198 20415-20422 VBN denotes induced
T21199 20423-20425 IN denotes by
T21200 20426-20429 NNP denotes TCR
T21201 20430-20437 NNS denotes signals
T21202 20438-20441 CC denotes and
T21203 20442-20444 VBZ denotes is
T21204 20445-20454 JJ denotes essential
T21205 20455-20458 IN denotes for
T21206 20459-20464 JJ denotes early
T21207 20465-20470 JJ denotes IFN-γ
T21208 20471-20481 NN denotes expression
T21209 20481-20482 . denotes .
T21210 20483-20488 NN denotes Runx3
T21211 20489-20491 VBZ denotes is
T21212 20492-20499 JJ denotes present
T21213 20500-20502 IN denotes in
T21214 20503-20508 JJ denotes naive
T21215 20509-20512 NNP denotes CD8
T21216 20512-20513 CD denotes +
T21217 20514-20515 NNP denotes T
T21218 20516-20521 NNS denotes cells
T21219 20522-20525 CC denotes and
T21220 20526-20535 VBZ denotes represses
T21221 20536-20541 NNP denotes Runx1
T21222 20542-20545 CC denotes and
T21223 20546-20553 VBZ denotes induces
T21224 20554-20559 NNP denotes Eomes
T21225 20559-20560 , denotes ,
T21226 20561-20569 NN denotes perforin
T21227 20569-20570 , denotes ,
T21228 20571-20579 NN denotes granzyme
T21229 20580-20581 NNP denotes B
T21230 20581-20582 , denotes ,
T21231 20583-20586 CC denotes and
T21232 20587-20592 NNP denotes IFN-γ
T21233 20593-20603 NN denotes expression
T21234 20603-20604 . denotes .
T21235 20605-20610 NNS denotes Eomes
T21236 20611-20614 MD denotes may
T21237 20615-20626 VB denotes participate
T21238 20627-20629 IN denotes in
T21239 20630-20640 VBG denotes sustaining
T21240 20641-20645 JJ denotes late
T21241 20646-20651 JJ denotes IFN-γ
T21242 20652-20662 NN denotes expression
T21243 20662-20663 , denotes ,
T21244 20664-20671 IN denotes whereas
T21245 20672-20677 NNP denotes Runx3
T21246 20678-20681 CC denotes and
T21247 20682-20687 NNP denotes Eomes
T21248 20688-20689 -LRB- denotes (
T21249 20689-20692 CC denotes but
T21250 20693-20696 RB denotes not
T21251 20697-20702 NNP denotes T-bet
T21252 20702-20703 -RRB- denotes )
T21253 20704-20707 MD denotes may
T21254 20708-20717 VB denotes cooperate
T21255 20718-20720 TO denotes to
T21256 20721-20729 VB denotes activate
T21257 20730-20738 JJ denotes perforin
T21258 20739-20749 NN denotes expression
T21259 20749-20750 . denotes .
T21260 20751-20754 DT denotes The
T21261 20755-20761 JJ denotes dotted
T16704 30637-30640 IN denotes per
T16705 30641-30645 RB denotes well
T16706 30646-30648 IN denotes in
T16707 30649-30656 JJ denotes 12-well
T16708 30657-30663 NNS denotes plates
T16709 30663-30664 , denotes ,
T16710 30665-30668 DT denotes the
T16711 30669-30676 NN denotes culture
T16712 30677-30682 NNS denotes media
T16713 30683-30686 VBD denotes was
T16714 30687-30694 VBN denotes removed
T16715 30695-30698 CC denotes and
T16716 30699-30707 VBN denotes replaced
T16717 30708-30712 IN denotes with
T16718 30713-30721 JJ denotes complete
T16719 30722-30727 NNS denotes media
T16720 30728-30740 VBN denotes supplemented
T17572 33613-33621 NNP denotes Northern
T17573 33622-33625 CC denotes and
T17574 33626-33633 NNP denotes Western
T17575 33634-33638 NN denotes blot
T17576 33639-33647 NNS denotes analyses
T17577 33647-33648 . denotes .
T17578 33649-33652 NNP denotes RNA
T17579 33653-33662 NN denotes isolation
T17580 33663-33666 CC denotes and
T17581 33667-33675 JJ denotes Northern
T17582 33676-33680 NN denotes blot
T17583 33681-33689 NN denotes analysis
T17584 33690-33693 VBD denotes was
T17585 33694-33703 VBN denotes performed
T17586 33704-33706 IN denotes as
T17587 33707-33717 RB denotes previously
T17588 33718-33727 VBN denotes described
T17589 33728-33729 -LRB- denotes (
T17590 33729-33731 CD denotes 29
T17591 33731-33732 -RRB- denotes )
T17592 33732-33733 . denotes .
T17593 33734-33736 IN denotes In
T17594 33737-33742 NN denotes brief
T17595 33742-33743 , denotes ,
T17596 33744-33746 CD denotes 10
T17597 33747-33749 NN denotes μg
T17598 33750-33752 IN denotes of
T17599 33753-33758 JJ denotes total
T17600 33759-33762 NNP denotes RNA
T17601 33763-33766 VBD denotes was
T17602 33767-33773 VBN denotes loaded
T17603 33774-33777 IN denotes per
T17604 33778-33782 NN denotes lane
T17605 33783-33786 CC denotes and
T17606 33787-33798 VBN denotes transferred
T17607 33799-33801 TO denotes to
T17608 33802-33812 RB denotes positively
T17609 33813-33820 VBN denotes charged
T20359 15819-15822 DT denotes the
T20360 15823-15832 VBN denotes indicated
T20361 15833-15840 JJ denotes genomic
T20362 15841-15848 NNS denotes regions
T20363 15849-15852 VBD denotes was
T20364 15853-15862 VBN denotes evaluated
T20365 15863-15865 IN denotes by
T20366 15866-15875 JJ denotes real-time
T20367 15876-15879 NNP denotes PCR
T20368 15880-15882 IN denotes of
T20369 15883-15886 NNP denotes DNA
T20370 15887-15891 IN denotes from
T20371 15892-15910 JJ denotes immunoprecipitated
T20372 15911-15914 CC denotes and
T20373 15915-15920 NN denotes input
T20374 15921-15930 NN denotes chromatin
T20375 15930-15931 . denotes .
T20376 15932-15935 DT denotes The
T20377 15936-15940 NNS denotes data
T20378 15941-15944 VBP denotes are
T20379 15945-15948 DT denotes the
T20380 15949-15954 NNS denotes means
T20381 15955-15957 IN denotes of
T20382 15958-15967 VBP denotes duplicate
T20383 15968-15980 NNS denotes measurements
T20384 15981-15985 IN denotes from
T20385 15986-15989 CD denotes two
T20386 15990-15999 NN denotes chromatin
T20387 16000-16012 NNS denotes preparations
T20388 16013-16017 IN denotes from
T20389 16018-16021 CD denotes two
T20390 16022-16033 JJ denotes independent
T20391 16034-16037 NNP denotes CD8
T20392 16037-16038 CD denotes +
T20967 19206-19211 JJ denotes Runx3
T20968 19212-19220 NNS denotes controls
T20969 19221-19226 NNP denotes Eomes
T21262 20762-20766 NN denotes line
T21263 20767-20776 VBZ denotes indicates
T21264 20777-20780 DT denotes the
T21265 20781-20788 JJ denotes partial
T21266 20789-20795 NN denotes effect
T21267 20796-20798 IN denotes of
T21268 20799-20804 NN denotes T-bet
T21269 20805-20815 NN denotes deficiency
T21270 20816-20818 IN denotes on
T21271 20819-20823 NNP denotes Gzmb
T21272 20824-20828 NNP denotes mRNA
T21273 20829-20832 CC denotes but
T21274 20833-20836 RB denotes not
T21275 20837-20845 VB denotes granzyme
T21276 20846-20847 NNP denotes B
T21277 20848-20855 NN denotes protein
T21278 20856-20866 NN denotes expression
T21279 20866-20867 . denotes .
R19 T88 T91 amod Runx3,proteins
R20 T89 T88 cc and,Runx3
R21 T90 T88 conj T-box,Runx3
R22 T91 T92 nsubj proteins,cooperate
R23 T92 T92 ROOT cooperate,cooperate
R24 T93 T94 aux to,establish
R25 T94 T92 xcomp establish,cooperate
R26 T95 T97 det the,program
R27 T96 T97 amod transcriptional,program
R28 T97 T94 dobj program,establish
R29 T98 T97 prep of,program
R30 T99 T100 compound effector,CTLs
R31 T100 T98 pobj CTLs,of
R32 T101 T110 nsubj Activation,induces
R33 T102 T101 prep of,Activation
R34 T103 T107 amod naive,cells
R35 T104 T107 nmod CD8,cells
R36 T105 T107 nummod +,cells
R37 T106 T107 compound T,cells
R38 T107 T102 pobj cells,of
R39 T108 T107 prep with,cells
R40 T109 T108 pobj antigen,with
R41 T110 T92 conj induces,cooperate
R42 T111 T112 poss their,differentiation
R43 T112 T110 dobj differentiation,induces
R122 T191 T192 compound γ,expression
R123 T192 T164 conj expression,induction
R124 T193 T164 punct ",",induction
R125 T194 T161 advmod respectively,characterized
R126 T195 T148 punct .,show
R127 T196 T200 prep In,demonstrate
R128 T197 T196 pobj addition,In
R129 T198 T200 punct ",",demonstrate
R130 T199 T200 nsubj we,demonstrate
R131 T200 T200 ROOT demonstrate,demonstrate
R132 T201 T203 det a,role
R133 T202 T203 amod critical,role
R134 T203 T200 dobj role,demonstrate
R135 T204 T203 prep for,role
R136 T205 T207 det the,factor
R137 T206 T207 compound transcription,factor
R138 T207 T204 pobj factor,for
R139 T208 T207 appos Runx3,factor
R140 T209 T208 prep in,Runx3
R141 T210 T211 compound CTL,differentiation
R142 T211 T209 pobj differentiation,in
R143 T212 T200 punct .,demonstrate
R144 T213 T214 nsubj Runx3,regulates
R145 T214 T214 ROOT regulates,regulates
R146 T215 T216 compound Eomes,expression
R147 T216 T214 dobj expression,regulates
R148 T217 T219 advmod as,as
R149 T218 T219 advmod well,as
R150 T219 T216 cc as,expression
R151 T220 T216 conj expression,expression
R152 T221 T216 prep of,expression
R153 T222 T224 nummod three,markers
R154 T223 T224 compound cardinal,markers
R155 T224 T221 pobj markers,of
R156 T225 T224 prep of,markers
R157 T226 T229 det the,program
R158 T227 T228 compound effector,CTL
R159 T228 T229 compound CTL,program
R160 T229 T225 pobj program,of
R161 T230 T224 punct :,markers
R162 T231 T224 appos IFN-γ,markers
R163 T232 T231 punct ",",IFN-γ
R164 T233 T231 conj perforin,IFN-γ
R165 T234 T233 punct ",",perforin
R166 T235 T233 cc and,perforin
R167 T236 T237 compound granzyme,B
R168 T237 T233 conj B,perforin
R169 T238 T214 punct .,regulates
R170 T239 T241 poss Our,point
R171 T240 T241 compound data,point
R172 T241 T241 ROOT point,point
R173 T242 T241 prep to,point
R174 T243 T244 det the,existence
R175 T244 T242 pobj existence,to
R176 T245 T244 prep of,existence
R177 T246 T249 det an,network
R178 T247 T249 amod elaborate,network
R179 T248 T249 compound transcriptional,network
R180 T249 T245 pobj network,of
R181 T250 T254 prep in,induces
R182 T251 T250 pobj which,in
R183 T252 T254 nsubj Runx3,induces
R184 T253 T254 advmod initially,induces
R185 T254 T249 relcl induces,network
R186 T255 T254 cc and,induces
R187 T256 T257 advmod then,cooperates
R188 T257 T254 conj cooperates,induces
R189 T258 T257 prep with,cooperates
R190 T259 T261 amod T-box,factors
R191 T260 T261 compound transcription,factors
R192 T261 T258 pobj factors,with
R193 T262 T263 aux to,regulate
R194 T263 T257 advcl regulate,cooperates
R195 T264 T265 compound gene,transcription
R196 T265 T263 dobj transcription,regulate
R197 T266 T263 prep in,regulate
R198 T267 T266 pcomp differentiating,in
R199 T268 T267 dobj CTLs,differentiating
R200 T269 T241 punct .,point
R714 T948 T951 punct ",",used
R715 T949 T951 nsubj we,used
R716 T950 T951 aux have,used
R717 T951 T951 ROOT used,used
R718 T952 T951 prep in,used
R719 T953 T954 amod vitro,systems
R720 T954 T952 pobj systems,in
R721 T955 T956 amod such,as
R722 T956 T954 prep as,systems
R723 T957 T956 pobj those,as
R724 T958 T957 acl developed,those
R725 T959 T960 aux to,study
R726 T960 T958 xcomp study,developed
R727 T961 T965 nmod CD4,differentiation
R728 T962 T965 nummod +,differentiation
R729 T963 T964 compound T,cell
R730 T964 T965 compound cell,differentiation
R731 T965 T960 dobj differentiation,study
R732 T966 T967 aux to,define
R733 T967 T960 advcl define,study
R734 T968 T970 det the,basis
R735 T969 T970 amod molecular,basis
R736 T970 T967 dobj basis,define
R737 T971 T970 prep of,basis
R738 T972 T974 compound effector,differentiation
R739 T973 T974 compound CTL,differentiation
R740 T974 T971 pobj differentiation,of
R741 T975 T974 punct (,differentiation
R742 T976 T974 appos "5, 6",differentiation
R743 T977 T974 punct ),differentiation
R744 T978 T951 punct .,used
R745 T979 T982 det The,factors
R746 T980 T982 amod T-box,factors
R747 T981 T982 compound transcription,factors
R44 T113 T110 prep into,induces
R45 T114 T117 nmod effector,lymphocytes
R46 T115 T117 amod cytolytic,lymphocytes
R47 T116 T117 compound T,lymphocytes
R48 T117 T113 pobj lymphocytes,into
R49 T118 T119 punct (,CTLs
R50 T119 T117 appos CTLs,lymphocytes
R51 T120 T117 punct ),lymphocytes
R52 T121 T110 punct .,induces
R53 T122 T123 nsubj CTLs,lyse
R54 T123 T123 ROOT lyse,lyse
R55 T124 T128 amod infected,cells
R56 T125 T124 cc or,infected
R57 T126 T124 conj aberrant,infected
R58 T127 T128 compound target,cells
R59 T128 T123 dobj cells,lyse
R60 T129 T123 prep by,lyse
R61 T130 T129 pobj exocytosis,by
R62 T131 T130 prep of,exocytosis
R63 T132 T133 amod lytic,granules
R64 T133 T131 pobj granules,of
R65 T134 T133 acl containing,granules
R66 T135 T138 det the,perforin
R67 T136 T138 amod pore-forming,perforin
R68 T137 T138 compound protein,perforin
R69 T138 T134 dobj perforin,containing
R70 T139 T138 cc and,perforin
R71 T140 T141 det a,family
R72 T141 T144 nsubj family,termed
R73 T142 T141 prep of,family
R74 T143 T142 pobj proteases,of
R75 T144 T123 conj termed,lyse
R76 T145 T144 dobj granzymes,termed
R77 T146 T144 punct .,termed
R78 T147 T148 nsubj We,show
R79 T148 T148 ROOT show,show
R80 T149 T153 mark that,occurs
R81 T150 T151 compound effector,CTL
R82 T151 T152 compound CTL,differentiation
R83 T152 T153 nsubj differentiation,occurs
R84 T153 T148 ccomp occurs,show
R85 T154 T153 prep in,occurs
R86 T155 T157 nummod two,phases
R87 T156 T157 amod sequential,phases
R88 T157 T154 pobj phases,in
R89 T158 T157 prep in,phases
R90 T159 T158 pobj vitro,in
R91 T160 T157 punct ",",phases
R92 T161 T157 acl characterized,phases
R93 T162 T161 agent by,characterized
R94 T163 T164 amod early,induction
R95 T164 T162 pobj induction,by
R96 T165 T164 prep of,induction
R97 T166 T165 pobj T-bet,of
R98 T167 T166 cc and,T-bet
R99 T168 T169 amod late,induction
R100 T169 T166 conj induction,T-bet
R101 T170 T169 prep of,induction
R102 T171 T170 pobj Eomesodermin,of
R103 T172 T171 punct (,Eomesodermin
R104 T173 T171 appos Eomes,Eomesodermin
R105 T174 T171 punct ),Eomesodermin
R106 T175 T178 punct ",",factors
R107 T176 T178 amod T-box,factors
R108 T177 T178 compound transcription,factors
R109 T178 T192 nmod factors,expression
R110 T179 T180 nsubj that,regulate
R111 T180 T178 relcl regulate,factors
R112 T181 T185 det the,phases
R113 T182 T185 amod early,phases
R114 T183 T182 cc and,early
R115 T184 T182 conj late,early
R116 T185 T180 dobj phases,regulate
R117 T186 T185 prep of,phases
R118 T187 T186 pobj interferon,of
R119 T188 T189 punct (,IFN
R120 T189 T178 appos IFN,factors
R121 T190 T178 punct ),factors
R644 T878 T882 amod Naive,cells
R645 T879 T882 nmod CD8,cells
R646 T880 T882 nummod +,cells
R647 T881 T882 compound T,cells
R648 T882 T883 nsubj cells,differentiate
R649 T883 T883 ROOT differentiate,differentiate
R650 T884 T883 prep into,differentiate
R651 T885 T886 compound effector,CTLs
R652 T886 T884 pobj CTLs,into
R653 T887 T886 prep with,CTLs
R654 T888 T889 det the,ability
R655 T889 T887 pobj ability,with
R656 T890 T891 aux to,lyse
R657 T891 T889 acl lyse,ability
R658 T892 T894 amod antigen-bearing,cells
R659 T893 T894 compound target,cells
R660 T894 T891 dobj cells,lyse
R661 T895 T891 prep by,lyse
R662 T896 T895 pobj exocytosis,by
R663 T897 T896 prep of,exocytosis
R664 T898 T899 compound lytic,granules
R665 T899 T897 pobj granules,of
R666 T900 T899 acl containing,granules
R667 T901 T900 dobj perforin,containing
R668 T902 T901 cc and,perforin
R669 T903 T901 conj granzymes,perforin
R670 T904 T903 punct ",",granzymes
R671 T905 T891 cc and,lyse
R672 T906 T907 aux to,produce
R673 T907 T891 conj produce,lyse
R674 T908 T909 amod inflammatory,cytokines
R675 T909 T907 dobj cytokines,produce
R676 T910 T911 amod such,as
R677 T911 T909 prep as,cytokines
R678 T912 T911 pobj IFN-γ,as
R679 T913 T912 cc and,IFN-γ
R680 T914 T912 conj TNF,IFN-γ
R681 T915 T907 prep upon,produce
R682 T916 T915 pobj restimulation,upon
R683 T917 T907 prep through,produce
R684 T918 T919 det the,TCR
R685 T919 T917 pobj TCR,through
R686 T920 T921 punct (,"1, 2"
R687 T921 T919 parataxis "1, 2",TCR
R688 T922 T919 punct ),TCR
R689 T923 T883 punct .,differentiate
R690 T924 T928 prep In,elucidated
R691 T925 T926 compound vivo,experiments
R692 T926 T928 nsubj experiments,elucidated
R693 T927 T928 aux have,elucidated
R694 T928 T928 ROOT elucidated,elucidated
R695 T929 T931 amod many,parameters
R696 T930 T931 amod critical,parameters
R697 T931 T928 dobj parameters,elucidated
R698 T932 T931 acl governing,parameters
R699 T933 T934 det the,development
R700 T934 T932 dobj development,governing
R701 T935 T934 cc and,development
R702 T936 T934 conj evolution,development
R703 T937 T934 prep of,development
R704 T938 T940 amod primary,responses
R705 T939 T940 compound CTL,responses
R706 T940 T937 pobj responses,of
R707 T941 T940 punct (,responses
R708 T942 T940 appos "3, 4",responses
R709 T943 T940 punct ),responses
R710 T944 T928 punct .,elucidated
R711 T945 T951 prep In,used
R712 T946 T947 det this,study
R713 T947 T945 pobj study,In
R776 T1010 T1009 prep of,deletion
R777 T1011 T1010 pobj the Tbx21,of
R778 T1012 T1013 punct (,encoding
R779 T1013 T1016 nmod encoding,and eomesodermin genes
R780 T1014 T1013 dobj T-bet,encoding
R781 T1015 T1016 punct ),and eomesodermin genes
R782 T1016 T1009 nmod and eomesodermin genes,deletion
R783 T1017 T1018 auxpass is,associated
R784 T1018 T1018 ROOT associated,associated
R785 T1019 T1018 prep with,associated
R786 T1020 T1022 det a,loss
R787 T1021 T1022 amod selective,loss
R788 T1022 T1019 pobj loss,with
R748 T982 T990 nsubjpass factors,needed
R749 T983 T990 nsubjpass Eomesodermin,needed
R750 T984 T985 punct (,Eomes
R751 T985 T983 appos Eomes,Eomesodermin
R752 T986 T985 punct ),Eomes
R753 T987 T983 cc and,Eomesodermin
R754 T988 T983 conj T-bet,Eomesodermin
R755 T989 T990 auxpass are,needed
R756 T990 T990 ROOT needed,needed
R757 T991 T990 prep for,needed
R758 T992 T993 amod important,aspects
R759 T993 T991 pobj aspects,for
R760 T994 T993 prep of,aspects
R761 T995 T994 pobj effector,of
R762 T996 T995 cc and,effector
R763 T997 T999 compound memory,differentiation
R764 T998 T999 compound CTL,differentiation
R765 T999 T995 conj differentiation,effector
R766 T1000 T1001 punct (,7
R767 T1001 T999 npadvmod 7,differentiation
R768 T1002 T990 punct ),needed
R769 T1003 T990 punct .,needed
R770 T1004 T1018 prep In,associated
R771 T1005 T1006 amod uninfected,mice
R772 T1006 T1004 pobj mice,In
R773 T1007 T1018 punct ",",associated
R774 T1008 T1009 compound compound,deletion
R775 T1009 T1018 nsubjpass deletion,associated
R1001 T1235 T1238 nmod CD8,cells
R1002 T1236 T1238 nummod +,cells
R1003 T1237 T1238 compound T,cells
R1004 T1238 T1233 pobj cells,in
R1005 T1239 T1238 punct (,cells
R1006 T1240 T1238 appos "10, 12",cells
R1007 T1241 T1238 punct ),cells
R1008 T1242 T1232 punct ",",expressed
R1009 T1243 T1232 cc and,expressed
R1010 T1244 T1232 conj Runx3-deficient,expressed
R1011 T1245 T1244 nummod CD8,Runx3-deficient
R789 T1023 T1022 prep of,loss
R790 T1024 T1027 nummod CD8,cells
R791 T1025 T1027 nummod +,cells
R792 T1026 T1027 compound T,cells
R793 T1027 T1023 pobj cells,of
R794 T1028 T1022 prep with,loss
R795 T1029 T1030 det an,IL-2Rβ
R796 T1030 T1028 pobj IL-2Rβ,with
R797 T1031 T1030 amod high,IL-2Rβ
R798 T1032 T1031 punct ",",high
R799 T1033 T1034 compound memory,phenotype
R800 T1034 T1030 appos phenotype,IL-2Rβ
R801 T1035 T1036 punct (,8
R802 T1036 T1034 parataxis 8,phenotype
R803 T1037 T1036 punct ),8
R804 T1038 T1018 punct .,associated
R805 T1039 T1040 npadvmod Mice,deficient
R806 T1040 T1050 nsubj deficient,impaired
R807 T1041 T1040 prep for,deficient
R808 T1042 T1043 det both,T-bet
R809 T1043 T1041 pobj T-bet,for
R810 T1044 T1043 cc and,T-bet
R811 T1045 T1043 conj Eomes,T-bet
R812 T1046 T1040 prep in,deficient
R813 T1047 T1048 compound T,cells
R814 T1048 T1046 pobj cells,in
R815 T1049 T1050 aux have,impaired
R816 T1050 T1050 ROOT impaired,impaired
R817 T1051 T1050 dobj expression,impaired
R818 T1052 T1051 prep of,expression
R819 T1053 T1054 amod cytolytic,mediators
R820 T1054 T1052 pobj mediators,of
R821 T1055 T1062 punct ",",fail
R822 T1056 T1062 nsubj manifest,fail
R823 T1057 T1059 amod poor,activity
R824 T1058 T1059 amod cytolytic,activity
R825 T1059 T1056 dobj activity,manifest
R826 T1060 T1056 punct ",",manifest
R827 T1061 T1056 cc and,manifest
R828 T1062 T1050 conj fail,impaired
R829 T1063 T1064 aux to,control
R830 T1064 T1062 xcomp control,fail
R831 T1065 T1068 amod acute,virus
R832 T1066 T1068 amod lymphocytic,virus
R833 T1067 T1068 compound choriomeningitis,virus
R834 T1068 T1069 compound virus,infection
R835 T1069 T1064 dobj infection,control
R836 T1070 T1071 punct (,9
R837 T1071 T1064 npadvmod 9,control
R838 T1072 T1062 punct ),fail
R839 T1073 T1050 punct .,impaired
R840 T1074 T1093 advmod Nevertheless,resolved
R841 T1075 T1093 punct ",",resolved
R842 T1076 T1078 det the,roles
R843 T1077 T1078 amod specific,roles
R844 T1078 T1093 nsubj roles,resolved
R845 T1079 T1078 prep of,roles
R846 T1080 T1079 pobj T-bet,of
R847 T1081 T1080 cc and,T-bet
R848 T1082 T1080 conj Eomes,T-bet
R849 T1083 T1082 prep in,Eomes
R850 T1084 T1085 amod clonal,expansion
R851 T1085 T1083 pobj expansion,in
R852 T1086 T1085 cc and,expansion
R853 T1087 T1088 compound CTL,differentiation
R854 T1088 T1085 conj differentiation,expansion
R855 T1089 T1093 aux have,resolved
R856 T1090 T1093 neg not,resolved
R857 T1091 T1093 advmod yet,resolved
R858 T1092 T1093 auxpass been,resolved
R859 T1093 T1101 ccomp resolved,known
R860 T1094 T1101 punct :,known
R861 T1095 T1101 prep in,known
R862 T1096 T1095 amod particular,in
R863 T1097 T1101 punct ",",known
R864 T1098 T1101 nsubjpass it,known
R865 T1099 T1101 auxpass is,known
R866 T1100 T1101 neg not,known
R867 T1101 T1101 ROOT known,known
R868 T1102 T1106 mark whether,function
R869 T1103 T1105 det these,factors
R870 T1104 T1105 compound transcription,factors
R871 T1105 T1106 nsubj factors,function
R872 T1106 T1101 ccomp function,known
R873 T1107 T1106 advmod redundantly,function
R874 T1108 T1109 aux to,control
R875 T1109 T1106 xcomp control,function
R876 T1110 T1115 amod effector,differentiation
R877 T1111 T1115 nmod CD8,differentiation
R878 T1112 T1115 nummod +,differentiation
R879 T1113 T1114 compound T,cell
R880 T1114 T1115 compound cell,differentiation
R881 T1115 T1109 dobj differentiation,control
R882 T1116 T1106 punct ",",function
R883 T1117 T1106 cc and,function
R884 T1118 T1120 mark whether,do
R885 T1119 T1120 nsubj they,do
R886 T1120 T1106 conj do,function
R887 T1121 T1122 advmod so,directly
R888 T1122 T1120 advmod directly,do
R889 T1123 T1120 prep by,do
R890 T1124 T1123 pcomp targeting,by
R891 T1125 T1127 amod specific,cytokine
R892 T1126 T1127 compound effector,cytokine
R893 T1127 T1124 dobj cytokine,targeting
R894 T1128 T1127 cc and,cytokine
R895 T1129 T1131 amod cytolytic,proteins
R896 T1130 T1131 compound "genes. Runx",proteins
R897 T1131 T1127 conj proteins,cytokine
R898 T1132 T1131 punct ",",proteins
R899 T1133 T1134 det a,family
R900 T1134 T1131 conj family,proteins
R901 T1135 T1134 prep of,family
R902 T1136 T1139 nummod three,factors
R903 T1137 T1139 amod DNA-binding,factors
R904 T1138 T1139 compound transcription,factors
R905 T1139 T1135 pobj factors,of
R906 T1140 T1139 punct ",",factors
R907 T1141 T1143 compound control,differentiation
R908 T1142 T1143 compound thymocyte,differentiation
R909 T1143 T1139 appos differentiation,factors
R910 T1144 T1143 cc and,differentiation
R911 T1145 T1148 det the,decision
R912 T1146 T1148 compound CD4/CD8,decision
R913 T1147 T1148 compound lineage,decision
R914 T1148 T1143 conj decision,differentiation
R915 T1149 T1151 punct (,13
R916 T1150 T1151 nummod 10,13
R917 T1151 T1148 appos 13,decision
R918 T1152 T1139 punct ),factors
R919 T1153 T1101 punct .,known
R920 T1154 T1159 nsubjpass Runx3,expressed
R921 T1155 T1154 cc and,Runx3
R922 T1156 T1157 compound perforin,mRNA
R923 T1157 T1154 conj mRNA,Runx3
R924 T1158 T1159 auxpass are,expressed
R925 T1159 T1159 ROOT expressed,expressed
R926 T1160 T1159 agent by,expressed
R927 T1161 T1160 pobj double-positive,by
R928 T1162 T1163 punct (,DP
R929 T1163 T1161 appos DP,double-positive
R930 T1164 T1163 punct ),DP
R931 T1165 T1161 conj thymocytes,double-positive
R932 T1166 T1165 cc and,thymocytes
R933 T1167 T1169 nummod CD8,single-positive
R934 T1168 T1169 nummod +,single-positive
R935 T1169 T1165 conj single-positive,thymocytes
R936 T1170 T1169 punct (,single-positive
R937 T1171 T1169 appos SP,single-positive
R938 T1172 T1169 punct ),single-positive
R939 T1173 T1165 conj thymocytes,thymocytes
R940 T1174 T1173 cc but,thymocytes
R941 T1175 T1176 neg not,in
R942 T1176 T1161 prep in,double-positive
R943 T1177 T1180 nummod CD4,cells
R944 T1178 T1180 nummod +,cells
R945 T1179 T1180 compound SP,cells
R946 T1180 T1176 pobj cells,in
R1012 T1246 T1248 nummod +,cells
R1013 T1247 T1248 compound T,cells
R1014 T1248 T1249 nsubj cells,show
R1015 T1249 T1232 conj show,expressed
R1016 T1250 T1252 amod reduced,activity
R1017 T1251 T1252 amod cytolytic,activity
R1018 T1252 T1249 dobj activity,show
R1019 T1253 T1252 punct (,activity
R1020 T1254 T1252 appos "12, 13",activity
R1021 T1255 T1252 punct ),activity
R1022 T1256 T1249 punct .,show
R1023 T1257 T1259 nsubj We,tested
R1024 T1258 T1259 advmod therefore,tested
R1025 T1259 T1271 ccomp tested,show
R1026 T1260 T1268 mark whether,report
R1027 T1261 T1266 amod Runx3,"differentiation. In"
R1028 T1262 T1265 amod influenced,cell
R1029 T1263 T1265 amod cytolytic,cell
R1030 T1264 T1265 compound T,cell
R1031 T1265 T1266 compound cell,"differentiation. In"
R1032 T1266 T1268 nsubj "differentiation. In",report
R1033 T1267 T1268 det this,report
R1034 T1268 T1259 dobj report,tested
R1035 T1269 T1271 punct ",",show
R1036 T1270 T1271 nsubj we,show
R1037 T1271 T1271 ROOT show,show
R1038 T1272 T1279 mark that,regulate
R1039 T1273 T1355 conj B,IFN-γ
R1040 T1274 T1277 amod Runx3,factors
R1041 T1275 T1274 cc and,Runx3
R1042 T1276 T1274 conj T-box,Runx3
R1043 T1277 T1279 nsubj factors,regulate
R1044 T1278 T1279 advmod synergistically,regulate
R1045 T1279 T1271 ccomp regulate,show
R1046 T1280 T1281 compound CTL,differentiation
R1047 T1281 T1279 dobj differentiation,regulate
R1048 T1282 T1281 cc and,differentiation
R1049 T1283 T1281 conj function,differentiation
R1050 T1284 T1271 punct .,show
R1051 T1285 T1287 nsubjpass T-bet,induced
R1052 T1286 T1287 auxpass is,induced
R1053 T1287 T1287 ROOT induced,induced
R1054 T1288 T1289 advmod quickly,upon
R1055 T1289 T1287 prep upon,induced
R1056 T1290 T1291 compound TCR,stimulation
R1057 T1291 T1289 pobj stimulation,upon
R1058 T1292 T1287 cc and,induced
R947 T1181 T1182 punct (,14
R948 T1182 T1159 npadvmod 14,expressed
R949 T1183 T1182 punct ),14
R950 T1184 T1159 punct .,expressed
R951 T1185 T1189 mark Although,expressed
R952 T1186 T1189 nsubjpass Runx3,expressed
R953 T1187 T1189 auxpass is,expressed
R954 T1188 T1189 neg not,expressed
R955 T1189 T1199 advcl expressed,is
R956 T1190 T1189 prep in,expressed
R957 T1191 T1195 amod naive,cells
R958 T1192 T1195 compound CD4,cells
R959 T1193 T1195 nummod +,cells
R960 T1194 T1195 compound T,cells
R961 T1195 T1190 pobj cells,in
R962 T1196 T1199 punct ",",is
R963 T1197 T1198 poss its,expression
R964 T1198 T1199 nsubj expression,is
R965 T1199 T1199 ROOT is,is
R966 T1200 T1199 acomp up-regulated,is
R967 T1201 T1199 prep during,is
R968 T1202 T1204 compound Th1,differentiation
R969 T1203 T1204 compound cell,differentiation
R970 T1204 T1201 pobj differentiation,during
R971 T1205 T1204 punct ",",differentiation
R972 T1206 T1204 cc and,differentiation
R973 T1207 T1208 amod Runx3,influences
R974 T1208 T1209 compound influences,Th1
R975 T1209 T1204 conj Th1,differentiation
R976 T1210 T1211 compound cell,differentiation
R977 T1211 T1209 dobj differentiation,Th1
R978 T1212 T1211 cc and,differentiation
R979 T1213 T1211 conj function,differentiation
R980 T1214 T1209 prep through,Th1
R981 T1215 T1216 amod direct,regulation
R982 T1216 T1214 pobj regulation,through
R983 T1217 T1216 prep of,regulation
R984 T1218 T1219 amod the Il4 and Ifng cytokine,genes
R985 T1219 T1217 pobj genes,of
R986 T1220 T1219 punct (,genes
R987 T1221 T1219 appos "15, 16",genes
R988 T1222 T1219 punct ),genes
R989 T1223 T1199 punct .,is
R990 T1224 T1232 prep In,expressed
R991 T1225 T1224 pobj contrast,In
R992 T1226 T1232 punct ",",expressed
R993 T1227 T1230 det all,proteins
R994 T1228 T1230 nummod three,proteins
R995 T1229 T1230 compound Runx,proteins
R996 T1230 T1232 nsubjpass proteins,expressed
R997 T1231 T1232 auxpass are,expressed
R998 T1232 T1232 ROOT expressed,expressed
R999 T1233 T1232 prep in,expressed
R1000 T1234 T1238 amod mature,cells
R1076 T1310 T1308 prep during,induced
R1077 T1311 T1310 pobj differentiation,during
R1078 T1312 T1308 cc and,induced
R1079 T1313 T1308 conj sustains,induced
R1080 T1314 T1315 amod IFN-γ,expression
R1081 T1315 T1313 dobj expression,sustains
R1082 T1316 T1287 punct .,induced
R1083 T1317 T1319 nsubjpass Runx3,required
R1084 T1318 T1319 auxpass is,required
R1085 T1319 T1319 ROOT required,required
R1086 T1320 T1319 prep for,required
R1087 T1321 T1320 pobj Eomes,for
R1088 T1322 T1321 cc and,Eomes
R1089 T1323 T1324 amod perforin,expression
R1090 T1324 T1321 conj expression,Eomes
R1091 T1325 T1319 punct ",",required
R1092 T1326 T1319 cc and,required
R1093 T1327 T1328 det both,Eomes
R1094 T1328 T1331 nsubj Eomes,bind
R1095 T1329 T1328 cc and,Eomes
R1096 T1330 T1328 conj Runx3,Eomes
R1097 T1331 T1341 nsubjpass bind,unaffected
R1098 T1332 T1331 prep at,bind
R1099 T1333 T1332 pobj the Prf1 locus,at
R1100 T1334 T1340 punct ;,is
R1101 T1335 T1340 prep in,is
R1102 T1336 T1335 pobj contrast,in
R1103 T1337 T1340 punct ",",is
R1104 T1338 T1339 amod perforin,expression
R1105 T1339 T1340 nsubj expression,is
R1106 T1340 T1341 auxpass is,unaffected
R1107 T1341 T1319 conj unaffected,required
R1108 T1342 T1341 prep in,unaffected
R1109 T1343 T1342 pobj T-bet,in
R1110 T1344 T1345 amod deficient,cells
R1111 T1345 T1341 dep cells,unaffected
R1112 T1346 T1341 punct .,unaffected
R1113 T1347 T1348 compound T,cells
R1114 T1348 T1352 nsubj cells,decreased
R1115 T1349 T1348 acl lacking,cells
R1116 T1350 T1351 amod Runx3,show
R1117 T1351 T1349 dobj show,lacking
R1118 T1352 T1352 ROOT decreased,decreased
R1119 T1353 T1352 dobj expression,decreased
R1120 T1354 T1353 prep of,expression
R1121 T1355 T1354 pobj IFN-γ,of
R1122 T1356 T1355 cc and,IFN-γ
R1123 T1357 T1273 compound granzyme,B
R1124 T1358 T1352 punct ",",decreased
R1125 T1359 T1352 cc and,decreased
R1126 T1360 T1362 nsubj Runx3,binds
R1127 T1361 T1362 advmod also,binds
R1128 T1362 T1352 conj binds,decreased
R1129 T1363 T1365 det the,regions
R1130 T1364 T1365 compound promoter,regions
R1131 T1365 T1362 dobj regions,binds
R1132 T1366 T1365 prep of,regions
R1133 T1367 T1366 pobj the Ifng and Gzmb genes,of
R1134 T1368 T1362 punct .,binds
R1135 T1369 T1373 advmod Collectively,provide
R1136 T1370 T1373 punct ",",provide
R1137 T1371 T1372 det these,results
R1138 T1372 T1373 nsubj results,provide
R1139 T1373 T1373 ROOT provide,provide
R1140 T1374 T1373 dobj evidence,provide
R1141 T1375 T1373 prep for,provide
R1142 T1376 T1379 det a,network
R1143 T1377 T1379 amod complex,network
R1144 T1378 T1379 amod transcriptional,network
R1145 T1379 T1375 pobj network,for
R1146 T1380 T1383 prep in,is
R1147 T1381 T1380 pobj which,in
R1148 T1382 T1383 nsubj Runx3,is
R1149 T1383 T1379 relcl is,network
R1150 T1384 T1386 det a,regulator
R1151 T1385 T1386 amod primary,regulator
R1152 T1386 T1383 attr regulator,is
R1153 T1387 T1386 appos of Gzmb expression,regulator
R1154 T1388 T1383 cc but,is
R1155 T1389 T1383 conj synergizes,is
R1156 T1390 T1389 prep with,synergizes
R1157 T1391 T1390 pobj T-bet,with
R1158 T1392 T1391 cc and,T-bet
R1159 T1393 T1391 conj Eomes,T-bet
R1160 T1394 T1393 punct ",",Eomes
R1161 T1395 T1393 conj respectively,Eomes
R1162 T1396 T1373 punct ",",provide
R1163 T1397 T1398 aux to,promote
R1164 T1398 T1373 advcl promote,provide
R1165 T1399 T1398 dobj transcription,promote
R1166 T1400 T1399 prep of,transcription
R1167 T1401 T1400 pobj the Ifng and Prf1 genes,of
R1168 T1402 T1373 punct .,provide
R2075 T2702 T2705 det A,system
R2076 T2703 T2704 compound cell,culture
R2077 T2704 T2705 compound culture,system
R2078 T2705 T2712 advcl system,used
R2079 T2706 T2707 aux to,monitor
R2080 T2707 T2705 acl monitor,system
R2081 T2708 T2709 compound effector,CTL
R2082 T2709 T2710 compound CTL,differentiation
R2083 T2710 T2707 dobj differentiation,monitor
R2084 T2711 T2712 nsubj We,used
R2085 T2712 T2712 ROOT used,used
R1059 T1293 T1294 auxpass is,required
R1060 T1294 T1287 conj required,induced
R1061 T1295 T1294 prep for,required
R1062 T1296 T1297 amod early,programming
R1063 T1297 T1295 pobj programming,for
R1064 T1298 T1297 prep of,programming
R1065 T1299 T1300 compound cytokine,production
R1066 T1300 T1298 pobj production,of
R1067 T1301 T1302 punct (,17
R1068 T1302 T1294 npadvmod 17,required
R1069 T1303 T1294 punct ),required
R1070 T1304 T1287 punct ",",induced
R1071 T1305 T1308 mark whereas,induced
R1072 T1306 T1308 nsubjpass Eomes,induced
R1073 T1307 T1308 auxpass is,induced
R1074 T1308 T1287 advcl induced,induced
R1075 T1309 T1308 advmod later,induced
R2193 T2820 T2819 dobj contamination,avoid
R2194 T2821 T2820 prep with,contamination
R2195 T2822 T2821 pobj proteins,with
R2196 T2823 T2822 cc and,proteins
R2197 T2824 T2825 amod nucleic,acids
R2198 T2825 T2822 conj acids,proteins
R2199 T2826 T2822 acl derived,proteins
R2200 T2827 T2826 prep from,derived
R2201 T2828 T2827 pobj APCs,from
R2202 T2829 T2806 punct .,chose
R2203 T2830 T2831 expl There,were
R2204 T2831 T2856 ccomp were,are
R2205 T2832 T2834 advmod only,differences
R2206 T2833 T2834 amod minor,differences
R2207 T2834 T2831 attr differences,were
R2208 T2835 T2834 prep in,differences
R2209 T2836 T2837 compound gene,expression
R2210 T2837 T2835 pobj expression,in
R2211 T2838 T2834 prep during,differences
R2212 T2839 T2838 pobj differentiation,during
R2213 T2840 T2839 acl induced,differentiation
R2214 T2841 T2840 agent by,induced
R2215 T2842 T2841 pobj antigen/APC,by
R2216 T2843 T2842 cc versus,antigen/APC
R2217 T2844 T2846 amod anti-CD3,anti-CD28
R2218 T2845 T2846 compound /,anti-CD28
R2219 T2846 T2842 conj anti-CD28,antigen/APC
R2220 T2847 T2846 punct ",",anti-CD28
R2221 T2848 T2846 cc and,anti-CD28
R2222 T2849 T2851 det the,conclusions
R2223 T2850 T2851 amod major,conclusions
R2224 T2851 T2846 conj conclusions,anti-CD28
R2225 T2852 T2851 acl presented,conclusions
R2226 T2853 T2852 prep in,presented
R2227 T2854 T2855 det this,report
R2228 T2855 T2853 pobj report,in
R2229 T2856 T2856 ROOT are,are
R2230 T2857 T2858 det the,same
R2231 T2858 T2856 attr same,are
R2232 T2859 T2856 prep for,are
R2233 T2860 T2862 det both,conditions
R2234 T2861 T2862 amod activating,conditions
R2235 T2862 T2859 pobj conditions,for
R2086 T2713 T2717 det a,system
R2087 T2714 T2717 amod simple,system
R2088 T2715 T2716 compound cell,culture
R2089 T2716 T2717 compound culture,system
R2090 T2717 T2712 dobj system,used
R2091 T2718 T2719 aux to,examine
R2092 T2719 T2712 xcomp examine,used
R2093 T2720 T2721 det the,kinetics
R2094 T2721 T2719 dobj kinetics,examine
R2095 T2722 T2721 prep of,kinetics
R2096 T2723 T2725 amod effector,expression
R2097 T2724 T2725 compound gene,expression
R2098 T2725 T2722 pobj expression,of
R2099 T2726 T2719 prep during,examine
R2100 T2727 T2731 compound CD8,differentiation
R2101 T2728 T2731 compound +,differentiation
R2102 T2729 T2730 compound T,cell
R2103 T2730 T2731 compound cell,differentiation
R2104 T2731 T2726 pobj differentiation,during
R2105 T2732 T2712 punct .,used
R2106 T2733 T2737 amod Naive,cells
R2107 T2734 T2737 nmod CD8,cells
R2108 T2735 T2737 nummod +,cells
R2109 T2736 T2737 compound T,cells
R2110 T2737 T2744 nsubjpass cells,activated
R2111 T2738 T2737 prep from,cells
R2112 T2739 T2740 nmod P14,TCR
R2113 T2740 T2742 nmod TCR,mice
R2114 T2741 T2742 amod transgenic,mice
R2115 T2742 T2738 pobj mice,from
R2116 T2743 T2744 auxpass were,activated
R2117 T2744 T2744 ROOT activated,activated
R2118 T2745 T2744 prep for,activated
R2119 T2746 T2747 nummod 2,d
R2120 T2747 T2745 pobj d,for
R2121 T2748 T2747 prep with,d
R2122 T2749 T2748 pobj anti-CD3,with
R2123 T2750 T2749 cc and,anti-CD3
R2124 T2751 T2749 conj anti-CD28,anti-CD3
R2125 T2752 T2751 cc or,anti-CD28
R2126 T2753 T2751 conj with,anti-CD28
R2127 T2754 T2755 amod splenic,APCs
R2128 T2755 T2753 pobj APCs,with
R2129 T2756 T2755 prep in,APCs
R2130 T2757 T2758 det the,presence
R2131 T2758 T2756 pobj presence,in
R2132 T2759 T2758 prep of,presence
R2133 T2760 T2761 amod Gp33,peptide
R2134 T2761 T2759 pobj peptide,of
R2135 T2762 T2744 punct ",",activated
R2136 T2763 T2744 cc and,activated
R2137 T2764 T2765 auxpass were,cultured
R2138 T2765 T2744 conj cultured,activated
R2139 T2766 T2765 prep in,cultured
R2140 T2767 T2766 pobj media,in
R2141 T2768 T2767 acl containing,media
R2142 T2769 T2770 nummod 100,U/ml
R2143 T2770 T2768 dobj U/ml,containing
R2144 T2771 T2770 prep of,U/ml
R2145 T2772 T2774 amod recombinant,IL-2
R2146 T2773 T2774 amod human,IL-2
R2147 T2774 T2771 pobj IL-2,of
R2148 T2775 T2776 punct (,rhIL-2
R2149 T2776 T2774 appos rhIL-2,IL-2
R2150 T2777 T2765 punct ),cultured
R2151 T2778 T2744 punct .,activated
R2152 T2779 T2780 nsubj We,used
R2153 T2780 T2806 ccomp used,chose
R2154 T2781 T2783 nmod TCR,mice
R2155 T2782 T2783 amod transgenic,mice
R2156 T2783 T2780 dobj mice,used
R2157 T2784 T2780 prep for,used
R2158 T2785 T2786 det these,experiments
R2159 T2786 T2784 pobj experiments,for
R2160 T2787 T2789 mark because,provide
R2161 T2788 T2789 nsubj they,provide
R2162 T2789 T2780 advcl provide,used
R2163 T2790 T2792 det a,source
R2164 T2791 T2792 amod reliable,source
R2165 T2792 T2789 dobj source,provide
R2166 T2793 T2792 prep of,source
R2167 T2794 T2797 nummod CD8,cells
R2168 T2795 T2797 nummod +,cells
R2169 T2796 T2797 compound T,cells
R2170 T2797 T2793 pobj cells,of
R2171 T2798 T2799 nsubj that,are
R2172 T2799 T2797 relcl are,cells
R2173 T2800 T2801 advmod truly,naive
R2174 T2801 T2799 acomp naive,are
R2175 T2802 T2806 punct ;,chose
R2176 T2803 T2806 advmod however,chose
R2177 T2804 T2806 punct ",",chose
R2178 T2805 T2806 nsubj we,chose
R2179 T2806 T2806 ROOT chose,chose
R2180 T2807 T2809 neg not,stimulate
R2181 T2808 T2809 aux to,stimulate
R2182 T2809 T2806 xcomp stimulate,chose
R2183 T2810 T2809 dobj cells,stimulate
R2184 T2811 T2809 prep with,stimulate
R2185 T2812 T2811 pobj antigen,with
R2186 T2813 T2809 prep in,stimulate
R2187 T2814 T2815 amod most,experiments
R2188 T2815 T2813 pobj experiments,in
R2189 T2816 T2819 mark so,avoid
R2190 T2817 T2819 mark as,avoid
R2191 T2818 T2819 aux to,avoid
R2192 T2819 T2809 advcl avoid,stimulate
R2268 T2895 T2884 punct ",",limited
R2269 T2896 T2897 det a,period
R2270 T2897 T2884 dobj period,limited
R2271 T2898 T2899 nsubj that,coincides
R2272 T2899 T2897 relcl coincides,period
R2273 T2900 T2899 prep with,coincides
R2274 T2901 T2902 amod clonal,expansion
R2275 T2902 T2900 pobj expansion,with
R2276 T2903 T2902 prep of,expansion
R2277 T2904 T2907 nummod CD8,cells
R2278 T2905 T2907 nummod +,cells
R2279 T2906 T2907 compound T,cells
R2280 T2907 T2903 pobj cells,of
R2281 T2908 T2902 prep after,expansion
R2282 T2909 T2908 pobj activation,after
R2283 T2910 T2909 prep in,activation
R2284 T2911 T2910 pobj vivo,in
R2285 T2912 T2884 punct .,limited
R2564 T3380 T3382 amod Distinct,kinetics
R2565 T3381 T3382 compound expression,kinetics
R2566 T3382 T3395 nsubj kinetics,revealed
R2567 T3383 T3382 prep of,kinetics
R2568 T3384 T3387 nmod perforin,B
R2569 T3385 T3384 cc and,perforin
R2570 T3386 T3387 compound granzyme,B
R2571 T3387 T3383 pobj B,of
R2572 T3388 T3382 prep during,kinetics
R2573 T3389 T3390 compound CTL,development
R2574 T3390 T3388 pobj development,during
R2575 T3391 T3390 prep in,development
R2576 T3392 T3391 pobj culture,in
R2577 T3393 T3394 poss Our,experiments
R2578 T3394 T3395 nsubj experiments,revealed
R2579 T3395 T3395 ROOT revealed,revealed
R2580 T3396 T3397 amod clear,differences
R2581 T3397 T3395 dobj differences,revealed
R2582 T3398 T3397 prep in,differences
R2583 T3399 T3400 det the,kinetics
R2584 T3400 T3398 pobj kinetics,in
R2585 T3401 T3400 prep of,kinetics
R2586 T3402 T3401 pobj perforin,of
R2587 T3403 T3397 punct ",",differences
R2588 T3404 T3405 compound granzyme,B
R2589 T3405 T3397 appos B,differences
R2590 T3406 T3405 punct ",",B
R2591 T3407 T3405 cc and,B
R2592 T3408 T3409 compound cytokine,expression
R2593 T3409 T3405 conj expression,B
R2594 T3410 T3397 prep during,differences
R2595 T3411 T3415 compound CD8,activation
R2596 T3412 T3415 compound +,activation
R2236 T2863 T2856 punct .,are
R2237 T2864 T2874 prep Under,expanded
R2238 T2865 T2867 poss our,conditions
R2239 T2866 T2867 compound culture,conditions
R2240 T2867 T2864 pobj conditions,Under
R2241 T2868 T2869 punct ",",activated
R2242 T2869 T2873 amod activated,cells
R2243 T2870 T2873 compound CD8,cells
R2244 T2871 T2873 nummod +,cells
R2245 T2872 T2873 compound T,cells
R2246 T2873 T2874 nsubj cells,expanded
R2247 T2874 T2874 ROOT expanded,expanded
R2248 T2875 T2874 advmod exponentially,expanded
R2249 T2876 T2874 cc and,expanded
R2250 T2877 T2874 conj accumulated,expanded
R2251 T2878 T2877 prep for,accumulated
R2252 T2879 T2878 pobj >,for
R2253 T2880 T2879 nummod 8,>
R2254 T2881 T2881 ROOT d,d
R2255 T2882 T2874 punct .,expanded
R2256 T2883 T2884 nsubj We,limited
R2257 T2884 T2884 ROOT limited,limited
R2258 T2885 T2886 poss our,analysis
R2259 T2886 T2884 dobj analysis,limited
R2260 T2887 T2884 prep to,limited
R2261 T2888 T2890 det the,6
R2262 T2889 T2890 amod first,6
R2263 T2890 T2892 dep 6,d
R2264 T2891 T2890 appos 8,6
R2265 T2892 T2887 pobj d,to
R2266 T2893 T2884 prep after,limited
R2267 T2894 T2893 pobj activation,after
R2744 T3560 T3559 prep of,fraction
R2745 T3561 T3563 amod naive,cells
R2746 T3562 T3563 compound T,cells
R2747 T3563 T3560 pobj cells,of
R2748 T3564 T3564 ROOT expressed,expressed
R2749 T3565 T3566 det the,cytokines
R2750 T3566 T3567 compound cytokines,IFN-γ
R2751 T3567 T3564 dobj IFN-γ,expressed
R2752 T3568 T3567 cc and,IFN-γ
R2753 T3569 T3567 conj TNF,IFN-γ
R2754 T3570 T3564 prep in,expressed
R2755 T3571 T3570 pobj response,in
R2756 T3572 T3571 prep to,response
R2757 T3573 T3572 pobj stimulation,to
R2758 T3574 T3564 punct ",",expressed
R2759 T3575 T3564 cc and,expressed
R2760 T3576 T3577 det this,capacity
R2761 T3577 T3578 nsubj capacity,increased
R2762 T3578 T3564 conj increased,expressed
R2763 T3579 T3578 advmod significantly,increased
R2764 T3580 T3578 prep in,increased
R2765 T3581 T3582 amod differentiated,cells
R2766 T3582 T3580 pobj cells,in
R2767 T3583 T3578 punct (,increased
R2768 T3584 T3586 nmod Fig.,E
R2769 T3585 T3586 nummod 1,E
R2770 T3586 T3588 ccomp E,see
R2771 T3587 T3588 punct ;,see
R2772 T3588 T3578 parataxis see,increased
R2597 T3413 T3415 compound T,activation
R2598 T3414 T3415 compound cell,activation
R2599 T3415 T3410 pobj activation,during
R2600 T3416 T3418 punct (,1
R2601 T3417 T3418 nmod Fig.,1
R2602 T3418 T3397 parataxis 1,differences
R2603 T3419 T3418 punct ),1
R2604 T3420 T3395 punct .,revealed
R2605 T3421 T3423 amod Naive,cells
R2606 T3422 T3423 compound T,cells
R2607 T3423 T3424 nsubj cells,showed
R2608 T3424 T3424 ROOT showed,showed
R2609 T3425 T3426 amod detectable,expression
R2610 T3426 T3424 dobj expression,showed
R2611 T3427 T3426 prep of,expression
R2612 T3428 T3429 compound perforin,mRNA
R2613 T3429 T3427 pobj mRNA,of
R2614 T3430 T3432 advmod as,as
R2615 T3431 T3432 advmod well,as
R2616 T3432 T3426 cc as,expression
R2617 T3433 T3434 compound perforin,protein
R2618 T3434 T3426 conj protein,expression
R2619 T3435 T3434 punct (,protein
R2620 T3436 T3434 parataxis Fig.,protein
R2621 T3437 T3436 nummod 1,Fig.
R2622 T3438 T3436 punct ",",Fig.
R2623 T3439 T3440 det A,D
R2624 T3440 T3436 appos D,Fig.
R2625 T3441 T3436 punct ),Fig.
R2626 T3442 T3424 punct .,showed
R2627 T3443 T3443 ROOT Relative,Relative
R2628 T3444 T3443 prep to,Relative
R2629 T3445 T3446 poss its,expression
R2630 T3446 T3444 pobj expression,to
R2631 T3447 T3446 prep in,expression
R2632 T3448 T3450 amod naive,cells
R2633 T3449 T3450 compound T,cells
R2634 T3450 T3447 pobj cells,in
R2635 T3451 T3443 punct ",",Relative
R2636 T3452 T3443 conj perforin,Relative
R2637 T3453 T3452 punct (,perforin
R2638 T3454 T3452 appos Prf1,perforin
R2639 T3455 T3452 punct ),perforin
R2640 T3456 T3457 compound mRNA,expression
R2641 T3457 T3460 nsubj expression,increase
R2642 T3458 T3460 aux did,increase
R2643 T3459 T3460 neg not,increase
R2644 T3460 T3460 ROOT increase,increase
R2645 T3461 T3460 advmod appreciably,increase
R2646 T3462 T3460 prep at,increase
R2647 T3463 T3462 pobj day,at
R2648 T3464 T3463 nummod 2,day
R2649 T3465 T3460 cc but,increase
R2650 T3466 T3460 conj showed,increase
R2651 T3467 T3469 det a,decrease
R2652 T3468 T3469 amod reproducible,decrease
R2653 T3469 T3466 dobj decrease,showed
R2654 T3470 T3469 prep at,decrease
R2655 T3471 T3470 pobj day,at
R2656 T3472 T3471 nummod 4,day
R2657 T3473 T3469 punct ",",decrease
R2658 T3474 T3466 conj followed,showed
R2659 T3475 T3474 agent by,followed
R2660 T3476 T3477 amod robust,reexpression
R2661 T3477 T3475 pobj reexpression,by
R2662 T3478 T3474 prep between,followed
R2663 T3479 T3478 pobj days,between
R2664 T3480 T3484 nummod 4,Fig.
R2665 T3481 T3480 cc and,4
R2666 T3482 T3480 conj 8,4
R2667 T3483 T3484 punct (,Fig.
R2668 T3484 T3474 npadvmod Fig.,followed
R2669 T3485 T3484 nummod 1,Fig.
R2670 T3486 T3484 punct ",",Fig.
R2671 T3487 T3484 appos A,Fig.
R2672 T3488 T3487 appos D,A
R2673 T3489 T3487 punct ),A
R2674 T3490 T3460 punct .,increase
R2675 T3491 T3491 ROOT In,In
R2676 T3492 T3491 pobj contrast,In
R2677 T3493 T3495 quantmod ",",B
R2678 T3494 T3495 compound granzyme,B
R2679 T3495 T3491 conj B,In
R2680 T3496 T3495 punct (,B
R2681 T3497 T3495 appos Gzmb,B
R2682 T3498 T3495 punct ),B
R2683 T3499 T3500 nsubj mRNA,was
R2684 T3500 T3500 ROOT was,was
R2685 T3501 T3500 acomp low,was
R2686 T3502 T3501 cc or,low
R2687 T3503 T3501 conj undetectable,low
R2688 T3504 T3503 prep in,undetectable
R2689 T3505 T3507 amod naive,cells
R2690 T3506 T3507 compound T,cells
R2691 T3507 T3504 pobj cells,in
R2692 T3508 T3500 cc but,was
R2693 T3509 T3500 conj was,was
R2694 T3510 T3511 advmod strongly,up-regulated
R2695 T3511 T3509 acomp up-regulated,was
R2696 T3512 T3511 prep by,up-regulated
R2697 T3513 T3512 pobj day,by
R2698 T3514 T3513 nummod 2,day
R2699 T3515 T3513 prep after,day
R2700 T3516 T3515 pobj stimulation,after
R2701 T3517 T3511 cc and,up-regulated
R2702 T3518 T3500 conj increased,was
R2703 T3519 T3518 advmod progressively,increased
R2704 T3520 T3518 prep until,increased
R2705 T3521 T3520 pobj day,until
R2706 T3522 T3521 nummod 6,day
R2707 T3523 T3524 punct (,Fig.
R2708 T3524 T3518 npadvmod Fig.,increased
R2709 T3525 T3524 nummod 1,Fig.
R2710 T3526 T3524 punct ",",Fig.
R2711 T3527 T3524 conj A,Fig.
R2712 T3528 T3527 cc and,A
R2713 T3529 T3527 conj B,A
R2714 T3530 T3529 punct ),B
R2715 T3531 T3518 punct ;,increased
R2716 T3532 T3538 advmod similarly,expressed
R2717 T3533 T3538 punct ",",expressed
R2718 T3534 T3536 compound granzyme,protein
R2719 T3535 T3536 compound B,protein
R2720 T3536 T3538 nsubjpass protein,expressed
R2721 T3537 T3538 auxpass was,expressed
R2722 T3538 T3538 ROOT expressed,expressed
R2723 T3539 T3538 prep by,expressed
R2724 T3540 T3539 pobj day,by
R2725 T3541 T3540 nummod 4,day
R2726 T3542 T3538 cc and,expressed
R2727 T3543 T3538 conj remained,expressed
R2728 T3544 T3543 acomp high,remained
R2729 T3545 T3543 prep until,remained
R2730 T3546 T3545 pobj day,until
R2731 T3547 T3546 nummod 6,day
R2732 T3548 T3549 punct (,Fig.
R2733 T3549 T3546 appos Fig.,day
R2734 T3550 T3549 nummod 1,Fig.
R2735 T3551 T3549 npadvmod E,Fig.
R2736 T3552 T3549 punct ),Fig.
R2737 T3553 T3500 punct .,was
R2738 T3554 T3555 mark As,expected
R2739 T3555 T3564 advcl expected,expressed
R2740 T3556 T3564 punct ",",expressed
R2741 T3557 T3559 det a,fraction
R2742 T3558 T3559 amod small,fraction
R2743 T3559 T3564 nsubj fraction,expressed
R2819 T3635 T3636 compound gene,expression
R2820 T3636 T3630 dobj expression,minimizes
R2821 T3637 T3630 prep during,minimizes
R2822 T3638 T3639 det the,period
R2823 T3639 T3637 pobj period,during
R2824 T3640 T3639 prep of,period
R2825 T3641 T3642 det the,assay
R2826 T3642 T3640 pobj assay,of
R2827 T3643 T3630 punct .,minimizes
R2828 T3644 T3646 amod Naive,cells
R2829 T3645 T3646 compound T,cells
R2773 T3589 T3590 advmod also,Fig.
R2774 T3590 T3592 dative Fig.,A
R2775 T3591 T3592 nummod 2,A
R2776 T3592 T3588 dobj A,see
R2777 T3593 T3588 punct ),see
R2778 T3594 T3578 punct .,increased
R2779 T3595 T3596 nsubj We,evaluated
R2780 T3596 T3596 ROOT evaluated,evaluated
R2781 T3597 T3599 amod antigen-dependent,function
R2782 T3598 T3599 compound cytolytic,function
R2783 T3599 T3596 dobj function,evaluated
R2784 T3600 T3596 prep in,evaluated
R2785 T3601 T3603 det a,assay
R2786 T3602 T3603 amod short-term,assay
R2787 T3603 T3600 pobj assay,in
R2788 T3604 T3610 prep in,measured
R2789 T3605 T3604 pobj which,in
R2790 T3606 T3608 compound target,death
R2791 T3607 T3608 compound cell,death
R2792 T3608 T3610 nsubjpass death,measured
R2793 T3609 T3610 auxpass was,measured
R2794 T3610 T3603 relcl measured,assay
R2795 T3611 T3610 prep within,measured
R2796 T3612 T3613 nummod 2,h
R2797 T3613 T3611 pobj h,within
R2798 T3614 T3617 punct (,F
R2799 T3615 T3617 nmod Fig.,F
R2800 T3616 T3615 nummod 1,Fig.
R2801 T3617 T3613 appos F,h
R2802 T3618 T3613 punct ),h
R2803 T3619 T3596 punct .,evaluated
R2804 T3620 T3630 prep By,minimizes
R2805 T3621 T3620 pcomp limiting,By
R2806 T3622 T3623 det the,duration
R2807 T3623 T3621 dobj duration,limiting
R2808 T3624 T3623 prep of,duration
R2809 T3625 T3626 compound TCR,stimulation
R2810 T3626 T3624 pobj stimulation,of
R2811 T3627 T3630 punct ",",minimizes
R2812 T3628 T3629 det this,strategy
R2813 T3629 T3630 nsubj strategy,minimizes
R2814 T3630 T3630 ROOT minimizes,minimizes
R2815 T3631 T3632 amod cytolysis,secondary
R2816 T3632 T3636 amod secondary,expression
R2817 T3633 T3636 nmod to,expression
R2818 T3634 T3636 amod new,expression
R2969 T3785 T3792 mark whereas,induced
R2970 T3786 T3787 amod perforin,expression
R2971 T3787 T3792 nsubjpass expression,induced
R2972 T3788 T3787 cc and,expression
R2973 T3789 T3790 amod cytolytic,function
R2974 T3790 T3787 conj function,expression
R2975 T3791 T3792 auxpass are,induced
R2976 T3792 T3782 advcl induced,programmed
R2977 T3793 T3792 advmod later,induced
R2978 T3794 T3792 punct ",",induced
R2979 T3795 T3792 prep during,induced
R2980 T3796 T3797 det the,phase
R2981 T3797 T3795 pobj phase,during
R2982 T3798 T3797 prep of,phase
R2983 T3799 T3800 amod clonal,expansion
R2984 T3800 T3798 pobj expansion,of
R2985 T3801 T3800 prep in,expansion
R2986 T3802 T3801 pobj IL-2,in
R2987 T3803 T3756 punct .,show
R2830 T3646 T3649 nsubj cells,display
R2831 T3647 T3649 aux did,display
R2832 T3648 T3649 neg not,display
R2833 T3649 T3649 ROOT display,display
R2834 T3650 T3652 amod significant,function
R2835 T3651 T3652 amod cytolytic,function
R2836 T3652 T3649 dobj function,display
R2837 T3653 T3652 prep in,function
R2838 T3654 T3656 det this,assay
R2839 T3655 T3656 amod short-term,assay
R2840 T3656 T3653 pobj assay,in
R2841 T3657 T3656 punct (,assay
R2842 T3658 T3659 amod unpublished,data
R2843 T3659 T3656 appos data,assay
R2844 T3660 T3656 punct ),assay
R2845 T3661 T3663 punct ",",likely
R2846 T3662 T3663 advmod most,likely
R2847 T3663 T3649 advmod likely,display
R2848 T3664 T3666 mark because,express
R2849 T3665 T3666 nsubj they,express
R2850 T3666 T3649 advcl express,display
R2851 T3667 T3671 amod immature,forms
R2852 T3668 T3671 punct (,forms
R2853 T3669 T3671 amod unprocessed,forms
R2854 T3670 T3671 punct ),forms
R2855 T3671 T3666 dobj forms,express
R2856 T3672 T3671 prep of,forms
R2857 T3673 T3672 pobj perforin,of
R2858 T3674 T3666 cc and,express
R2859 T3675 T3666 conj lack,express
R2860 T3676 T3677 det the,capacity
R2861 T3677 T3675 dobj capacity,lack
R2862 T3678 T3679 aux to,degranulate
R2863 T3679 T3677 acl degranulate,capacity
R2864 T3680 T3683 punct (,19
R2865 T3681 T3683 nummod 18,19
R2866 T3682 T3683 punct ",",19
R2867 T3683 T3679 dobj 19,degranulate
R2868 T3684 T3679 punct ),degranulate
R2869 T3685 T3649 punct .,display
R2870 T3686 T3687 advmod Even,after
R2871 T3687 T3697 prep after,showed
R2872 T3688 T3687 pobj activation,after
R2873 T3689 T3688 prep for,activation
R2874 T3690 T3689 pobj 2,for
R2875 T3691 T3690 cc or,2
R2876 T3692 T3690 conj 4,2
R2877 T3693 T3697 meta d,showed
R2878 T3694 T3697 punct ",",showed
R2879 T3695 T3696 det the,cells
R2880 T3696 T3697 nsubj cells,showed
R2881 T3697 T3697 ROOT showed,showed
R2882 T3698 T3700 amod poor,activity
R2883 T3699 T3700 amod cytolytic,activity
R2884 T3700 T3697 dobj activity,showed
R2885 T3701 T3704 punct (,F
R2886 T3702 T3704 nmod Fig.,F
R2887 T3703 T3702 nummod 1,Fig.
R2888 T3704 T3700 appos F,activity
R2889 T3705 T3700 punct ),activity
R2890 T3706 T3697 punct ",",showed
R2891 T3707 T3697 prep in,showed
R2892 T3708 T3709 amod striking,contrast
R2893 T3709 T3707 pobj contrast,in
R2894 T3710 T3709 prep to,contrast
R2895 T3711 T3712 poss their,capacity
R2896 T3712 T3710 pobj capacity,to
R2897 T3713 T3712 prep for,capacity
R2898 T3714 T3716 amod efficient,production
R2899 T3715 T3716 compound cytokine,production
R2900 T3716 T3713 pobj production,for
R2901 T3717 T3716 punct (,production
R2902 T3718 T3720 nmod Fig.,E
R2903 T3719 T3720 nummod 1,E
R2904 T3720 T3716 appos E,production
R2905 T3721 T3716 punct ),production
R2906 T3722 T3697 punct .,showed
R2907 T3723 T3724 amod Only,cells
R2908 T3724 T3725 nsubj cells,cultured
R2909 T3725 T3725 ROOT cultured,cultured
R2910 T3726 T3729 mark until,displayed
R2911 T3727 T3726 pobj day,until
R2912 T3728 T3727 nummod 6,day
R2913 T3729 T3725 advcl displayed,cultured
R2914 T3730 T3731 amod robust,cytotoxicity
R2915 T3731 T3729 dobj cytotoxicity,displayed
R2916 T3732 T3729 punct ",",displayed
R2917 T3733 T3734 mark as,judged
R2918 T3734 T3729 advcl judged,displayed
R2919 T3735 T3734 agent by,judged
R2920 T3736 T3737 poss their,ability
R2921 T3737 T3735 pobj ability,by
R2922 T3738 T3739 aux to,induce
R2923 T3739 T3737 acl induce,ability
R2924 T3740 T3739 dobj apoptosis,induce
R2925 T3741 T3739 prep in,induce
R2926 T3742 T3744 det a,number
R2927 T3743 T3744 amod large,number
R2928 T3744 T3741 pobj number,in
R2929 T3745 T3744 prep of,number
R2930 T3746 T3747 compound target,cells
R2931 T3747 T3745 pobj cells,of
R2932 T3748 T3744 punct (,number
R2933 T3749 T3751 nmod Fig.,F
R2934 T3750 T3749 nummod 1,Fig.
R2935 T3751 T3744 appos F,number
R2936 T3752 T3744 punct ),number
R2937 T3753 T3725 punct .,cultured
R2938 T3754 T3755 det These,results
R2939 T3755 T3756 nsubj results,show
R2940 T3756 T3756 ROOT show,show
R2941 T3757 T3782 mark that,programmed
R2942 T3758 T3782 prep after,programmed
R2943 T3759 T3762 det a,stimulus
R2944 T3760 T3762 amod strong,stimulus
R2945 T3761 T3762 amod priming,stimulus
R2946 T3762 T3758 pobj stimulus,after
R2947 T3763 T3762 prep through,stimulus
R2948 T3764 T3763 pobj TCRs,through
R2949 T3765 T3764 cc and,TCRs
R2950 T3766 T3767 amod co-stimulatory,receptors
R2951 T3767 T3764 conj receptors,TCRs
R2952 T3768 T3762 prep in,stimulus
R2953 T3769 T3773 amod vitro,expression
R2954 T3770 T3773 punct ",",expression
R2955 T3771 T3773 amod granzyme,expression
R2956 T3772 T3773 compound B,expression
R2957 T3773 T3768 pobj expression,in
R2958 T3774 T3773 cc and,expression
R2959 T3775 T3776 det the,ability
R2960 T3776 T3773 conj ability,expression
R2961 T3777 T3778 aux to,produce
R2962 T3778 T3776 acl produce,ability
R2963 T3779 T3780 compound effector,cytokines
R2964 T3780 T3782 nsubjpass cytokines,programmed
R2965 T3781 T3782 auxpass are,programmed
R2966 T3782 T3756 ccomp programmed,show
R2967 T3783 T3782 advmod early,programmed
R2968 T3784 T3782 punct ",",programmed
R3731 T4777 T4778 amod Distinct,kinetics
R3732 T4778 T4783 nsubj kinetics,expression
R3733 T4779 T4778 prep of,kinetics
R3734 T4780 T4779 pobj T-bet,of
R3735 T4781 T4780 cc and,T-bet
R3736 T4782 T4780 conj Eomes,T-bet
R3737 T4783 T4783 ROOT expression,expression
R3738 T4784 T4783 prep during,expression
R3739 T4785 T4786 compound CTL,differentiation
R3740 T4786 T4784 pobj differentiation,during
R3741 T4787 T4790 det The,factors
R3742 T4788 T4790 amod T-box,factors
R3743 T4789 T4790 compound transcription,factors
R3744 T4790 T4796 nsubjpass factors,linked
R3745 T4791 T4796 nsubjpass T-bet,linked
R3746 T4792 T4791 cc and,T-bet
R3747 T4793 T4791 conj Eomes,T-bet
R3748 T4794 T4796 aux have,linked
R3749 T4795 T4796 auxpass been,linked
R2988 T3804 T3820 advmod Therefore,are
R2989 T3805 T3820 punct ",",are
R2990 T3806 T3810 det the,functions
R2991 T3807 T3810 nummod two,functions
R2992 T3808 T3810 amod major,functions
R2993 T3809 T3810 compound effector,functions
R2994 T3810 T3820 nsubj functions,are
R2995 T3811 T3810 prep of,functions
R2996 T3812 T3811 pobj CTL,of
R2997 T3813 T3812 punct ",",CTL
R2998 T3814 T3815 amod cytokine,production
R2999 T3815 T3818 nmod production,activity
R3000 T3816 T3815 cc and,production
R3001 T3817 T3818 compound cytolytic,activity
R3002 T3818 T3812 appos activity,CTL
R3003 T3819 T3820 punct ",",are
R3004 T3820 T3820 ROOT are,are
R3005 T3821 T3820 neg not,are
R3006 T3822 T3823 advmod intrinsically,coregulated
R3007 T3823 T3820 acomp coregulated,are
R3008 T3824 T3820 punct .,are
R3838 T4884 T4887 intj Fig.,A
R3839 T4885 T4884 nummod 1,Fig.
R3840 T4886 T4884 punct ",",Fig.
R3841 T4887 T4877 parataxis A,expressed
R3842 T4888 T4887 cc and,A
R3843 T4889 T4887 conj C,A
R3844 T4890 T4891 punct ;,quantified
R3845 T4891 T4854 conj quantified,were
R3846 T4892 T4891 prep in,quantified
R3847 T4893 T4892 pobj Fig.,in
R3848 T4894 T4893 nummod 1,Fig.
R3849 T4895 T4893 punct ",",Fig.
R3850 T4896 T4893 conj B,Fig.
R3851 T4897 T4896 cc and,B
R3852 T4898 T4896 conj D,B
R3853 T4899 T4891 punct ),quantified
R3854 T4900 T4854 punct .,were
R3855 T4901 T4906 prep In,was
R3856 T4902 T4901 pobj contrast,In
R3857 T4903 T4906 punct ",",was
R3858 T4904 T4905 compound Eomes,expression
R3859 T4905 T4906 nsubj expression,was
R3860 T4906 T4906 ROOT was,was
R3861 T4907 T4906 acomp low,was
R3862 T4908 T4907 cc or,low
R3863 T4909 T4907 conj undetectable,low
R3864 T4910 T4906 prep at,was
R3865 T4911 T4913 preconj both,mRNA
R3866 T4912 T4913 det the,mRNA
R3867 T4913 T4926 nsubj mRNA,priming
R3868 T4914 T4913 cc and,mRNA
R3869 T4915 T4916 compound protein,levels
R3870 T4916 T4913 conj levels,mRNA
R3871 T4917 T4916 prep in,levels
R3872 T4918 T4922 amod naive,cells
R3873 T4919 T4922 nmod CD8,cells
R3874 T4920 T4922 nummod +,cells
R3875 T4921 T4922 compound T,cells
R3876 T4922 T4917 pobj cells,in
R3877 T4923 T4916 punct ",",levels
R3878 T4924 T4913 cc and,mRNA
R3879 T4925 T4913 conj TCR,mRNA
R3880 T4926 T4910 pcomp priming,at
R3881 T4927 T4926 prep in,priming
R3882 T4928 T4927 pobj culture,in
R3883 T4929 T4906 conj had,was
R3884 T4930 T4933 advmod only,effect
R3750 T4796 T4796 ROOT linked,linked
R3751 T4797 T4796 prep to,linked
R3752 T4798 T4799 det the,regulation
R3753 T4799 T4797 pobj regulation,to
R3754 T4800 T4799 prep of,regulation
R3755 T4801 T4800 pobj genes,of
R3756 T4802 T4801 acl encoding,genes
R3757 T4803 T4804 compound effector,cytokines
R3758 T4804 T4802 dobj cytokines,encoding
R3759 T4805 T4804 punct (,cytokines
R3760 T4806 T4804 appos e.g.,cytokines
R3761 T4807 T4806 punct ",",e.g.
R3762 T4808 T4806 amod Ifng,e.g.
R3763 T4809 T4804 punct ),cytokines
R3764 T4810 T4804 cc and,cytokines
R3765 T4811 T4804 conj genes,cytokines
R3766 T4812 T4804 amod important,cytokines
R3767 T4813 T4812 prep for,important
R3768 T4814 T4815 amod cytolytic,function
R3769 T4815 T4813 pobj function,for
R3770 T4816 T4817 punct (,e.g.
R3771 T4817 T4819 acl e.g.,Prf1
R3772 T4818 T4817 punct ",",e.g.
R3773 T4819 T4799 appos Prf1,regulation
R3774 T4820 T4819 cc and,Prf1
R3775 T4821 T4819 conj GzmB,Prf1
R3776 T4822 T4819 punct ),Prf1
R3777 T4823 T4819 punct (,Prf1
R3778 T4824 T4819 appos 20,Prf1
R3779 T4825 T4819 punct ),Prf1
R3780 T4826 T4796 punct .,linked
R3781 T4827 T4828 nsubj We,investigated
R3782 T4828 T4828 ROOT investigated,investigated
R3783 T4829 T4830 det the,kinetics
R3784 T4830 T4828 dobj kinetics,investigated
R3785 T4831 T4830 prep of,kinetics
R3786 T4832 T4831 pobj expression,of
R3787 T4833 T4832 prep of,expression
R3788 T4834 T4836 det these,factors
R3789 T4835 T4836 compound transcription,factors
R3790 T4836 T4833 pobj factors,of
R3791 T4837 T4830 prep in,kinetics
R3792 T4838 T4839 poss our,in
R3793 T4839 T4837 conj in,in
R3794 T4840 T4841 amod vitro,cultures
R3795 T4841 T4839 pobj cultures,in
R3796 T4842 T4830 punct (,kinetics
R3797 T4843 T4828 npadvmod Fig.,investigated
R3798 T4844 T4843 nummod 1,Fig.
R3799 T4845 T4843 punct ",",Fig.
R3800 T4846 T4847 det A,D
R3801 T4847 T4843 appos D,Fig.
R3802 T4848 T4847 punct ),D
R3803 T4849 T4843 punct .,Fig.
R3804 T4850 T4851 compound T-bet,mRNA
R3805 T4851 T4854 nsubj mRNA,were
R3806 T4852 T4851 cc and,mRNA
R3807 T4853 T4851 conj protein,mRNA
R3808 T4854 T4854 ROOT were,were
R3809 T4855 T4854 neg not,were
R3810 T4856 T4854 acomp detectable,were
R3811 T4857 T4856 prep in,detectable
R3812 T4858 T4862 amod naive,cells
R3813 T4859 T4862 nmod CD8,cells
R3814 T4860 T4862 nummod +,cells
R3815 T4861 T4862 compound T,cells
R3816 T4862 T4857 pobj cells,in
R3817 T4863 T4854 punct ",",were
R3818 T4864 T4854 cc but,were
R3819 T4865 T4867 auxpass were,induced
R3820 T4866 T4867 advmod strongly,induced
R3821 T4867 T4854 conj induced,were
R3822 T4868 T4867 prep upon,induced
R3823 T4869 T4870 compound TCR,priming
R3824 T4870 T4867 npadvmod priming,induced
R3825 T4871 T4872 punct (,day
R3826 T4872 T4870 parataxis day,priming
R3827 T4873 T4872 nummod 2,day
R3828 T4874 T4872 punct ),day
R3829 T4875 T4867 cc and,induced
R3830 T4876 T4867 conj remained,induced
R3831 T4877 T4867 conj expressed,induced
R3832 T4878 T4877 prep through,expressed
R3833 T4879 T4878 pobj day,through
R3834 T4880 T4879 nummod 6,day
R3835 T4881 T4879 prep of,day
R3836 T4882 T4881 pobj differentiation,of
R3837 T4883 T4887 punct (,A
R3913 T4959 T4958 prep at,observed
R3914 T4960 T4959 pobj day,at
R3915 T4961 T4960 nummod 4,day
R3916 T4962 T4959 cc and,at
R3917 T4963 T4959 conj later,at
R3918 T4964 T4958 punct (,observed
R3919 T4965 T4958 npadvmod Fig.,observed
R3920 T4966 T4965 nummod 1,Fig.
R3921 T4967 T4965 punct ",",Fig.
R3922 T4968 T4965 conj A,Fig.
R3923 T4969 T4968 cc and,A
R3924 T4970 T4968 conj C,A
R3925 T4971 T4970 punct ),C
R3926 T4972 T4958 punct .,observed
R3927 T4973 T4974 compound T-bet,mRNA
R3928 T4974 T4975 compound mRNA,expression
R3929 T4975 T4977 nsubj expression,preceded
R3930 T4976 T4977 advmod slightly,preceded
R3931 T4977 T4991 ccomp preceded,expressed
R3932 T4978 T4979 det the,expression
R3933 T4979 T4977 dobj expression,preceded
R3934 T4980 T4979 prep of,expression
R3935 T4981 T4982 compound GzmB,mRNA
R3936 T4982 T4980 pobj mRNA,of
R3937 T4983 T4977 punct ;,preceded
R3938 T4984 T4991 advmod similarly,expressed
R3939 T4985 T4991 punct ",",expressed
R3940 T4986 T4987 compound Eomes,mRNA
R3941 T4987 T4991 nsubjpass mRNA,expressed
R3942 T4988 T4987 cc and,mRNA
R3943 T4989 T4987 conj protein,mRNA
R3944 T4990 T4991 auxpass were,expressed
R3945 T4991 T4991 ROOT expressed,expressed
R3946 T4992 T4991 advmod ∼,expressed
R3947 T4993 T4992 nummod 1,∼
R3948 T4994 T5006 meta d,(
R3949 T4995 T4994 advmod ahead,d
R3950 T4996 T4995 prep of,ahead
R3951 T4997 T4998 det the,reexpression
R3952 T4998 T4996 pobj reexpression,of
R3953 T4999 T4998 prep of,reexpression
R3954 T5000 T5001 compound perforin,mRNA
R3955 T5001 T4999 pobj mRNA,of
R3956 T5002 T5001 cc and,mRNA
R3957 T5003 T5001 conj protein,mRNA
R3958 T5004 T5005 punct ",",respectively
R3959 T5005 T4995 conj respectively,ahead
R3960 T5006 T5006 ROOT (,(
R3961 T5007 T5007 ROOT Fig.,Fig.
R3962 T5008 T5007 nummod 1,Fig.
R3963 T5009 T5010 punct ",",B
R3964 T5010 T5007 conj B,Fig.
R3965 T5011 T5010 cc and,B
R3966 T5012 T5010 conj D,B
R3967 T5013 T5007 punct ),Fig.
R3968 T5014 T5006 punct .,(
R3969 T5015 T5018 det This,analysis
R3970 T5016 T5018 amod detailed,analysis
R3971 T5017 T5018 amod kinetic,analysis
R3972 T5018 T5019 nsubj analysis,suggested
R3973 T5019 T5019 ROOT suggested,suggested
R3974 T5020 T5030 mark that,contribute
R3975 T5021 T5030 punct ",",contribute
R3976 T5022 T5030 prep under,contribute
R3977 T5023 T5025 poss our,conditions
R3978 T5024 T5025 compound culture,conditions
R3979 T5025 T5022 pobj conditions,under
R3980 T5026 T5030 punct ",",contribute
R3981 T5027 T5030 nsubj T-bet,contribute
R3982 T5028 T5027 cc and,T-bet
R3885 T4931 T4933 det a,effect
R3886 T4932 T4933 amod modest,effect
R3887 T4933 T4929 dobj effect,had
R3888 T4934 T4933 prep on,effect
R3889 T4935 T4936 poss its,expression
R3890 T4936 T4934 pobj expression,on
R3891 T4937 T4936 prep at,expression
R3892 T4938 T4937 pobj day,at
R3893 T4939 T4938 nummod 2,day
R3894 T4940 T4941 punct (,Fig.
R3895 T4941 T4938 appos Fig.,day
R3896 T4942 T4941 nummod 1,Fig.
R3897 T4943 T4941 punct ",",Fig.
R3898 T4944 T4941 conj A,Fig.
R3899 T4945 T4944 cc and,A
R3900 T4946 T4944 conj C,A
R3901 T4947 T4946 punct ),C
R3902 T4948 T4906 punct .,was
R3903 T4949 T4950 amod Strong,induction
R3904 T4950 T4958 nsubjpass induction,observed
R3905 T4951 T4950 prep of,induction
R3906 T4952 T4953 compound Eomes,mRNA
R3907 T4953 T4951 pobj mRNA,of
R3908 T4954 T4953 cc and,mRNA
R3909 T4955 T4958 nsubjpass protein,observed
R3910 T4956 T4958 auxpass was,observed
R3911 T4957 T4958 advmod only,observed
R3912 T4958 T4958 ROOT observed,observed
R4062 T5108 T5109 det the,period
R4063 T5109 T5107 pobj period,during
R4064 T5110 T5109 prep of,period
R4065 T5111 T5112 amod clonal,expansion
R4066 T5112 T5110 pobj expansion,of
R4067 T5113 T5112 prep in,expansion
R4068 T5114 T5113 pobj IL-2,in
R4069 T5115 T5106 cc and,induced
R4070 T5116 T5106 conj activates,induced
R4071 T5117 T5118 amod perforin,expression
R4072 T5118 T5116 dobj expression,activates
R4073 T5119 T5106 punct .,induced
R4875 T6191 T6190 pobj activation,upon
R4876 T6192 T6193 punct (,Fig.
R4877 T6193 T6187 dep Fig.,produced
R4878 T6194 T6195 nummod 2,A
R4879 T6195 T6193 dobj A,Fig.
R4880 T6196 T6195 punct ),A
R4881 T6197 T6187 punct .,produced
R4882 T6198 T6208 advmod Notably,observed
R4883 T6199 T6208 punct ",",observed
R4884 T6200 T6202 det this,effect
R4885 T6201 T6202 amod deleterious,effect
R4886 T6202 T6208 nsubjpass effect,observed
R4887 T6203 T6202 prep of,effect
R4888 T6204 T6205 compound T-bet,deficiency
R4889 T6205 T6203 pobj deficiency,of
R4890 T6206 T6208 auxpass was,observed
R4891 T6207 T6208 advmod only,observed
R4892 T6208 T6208 ROOT observed,observed
R4893 T6209 T6208 prep in,observed
R4894 T6210 T6209 pcomp differentiating,in
R4895 T6211 T6214 compound CD8,cells
R4896 T6212 T6214 nummod +,cells
R4897 T6213 T6214 compound T,cells
R4898 T6214 T6210 dobj cells,differentiating
R4899 T6215 T6210 prep until,differentiating
R4900 T6216 T6215 pobj day,until
R4901 T6217 T6216 nummod 4,day
R4902 T6218 T6216 prep of,day
R4903 T6219 T6218 pobj culture,of
R4904 T6220 T6208 cc but,observed
R3983 T5029 T5027 conj Eomes,T-bet
R3984 T5030 T5019 ccomp contribute,suggested
R3985 T5031 T5030 prep to,contribute
R3986 T5032 T5033 amod distinct,aspects
R3987 T5033 T5031 pobj aspects,to
R3988 T5034 T5033 prep of,aspects
R3989 T5035 T5036 compound gene,transcription
R3990 T5036 T5034 pobj transcription,of
R3991 T5037 T5031 prep during,to
R3992 T5038 T5039 compound CTL,differentiation
R3993 T5039 T5037 pobj differentiation,during
R3994 T5040 T5019 punct .,suggested
R3995 T5041 T5043 nsubjpass T-bet,required
R3996 T5042 T5043 auxpass is,required
R3997 T5043 T5043 ROOT required,required
R3998 T5044 T5043 advmod early,required
R3999 T5045 T5044 prep for,early
R4000 T5046 T5047 amod IFN-γ,production
R4001 T5047 T5045 pobj production,for
R4002 T5048 T5043 punct ",",required
R4003 T5049 T5043 cc and,required
R4004 T5050 T5051 poss our,data
R4005 T5051 T5052 nsubj data,suggested
R4006 T5052 T5043 conj suggested,required
R4007 T5053 T5057 mark that,function
R4008 T5054 T5057 nsubj Eomes,function
R4009 T5055 T5057 aux might,function
R4010 T5056 T5057 neg not,function
R4011 T5057 T5052 ccomp function,suggested
R4012 T5058 T5057 prep during,function
R4013 T5059 T5061 det this,period
R4014 T5060 T5061 amod early,period
R4015 T5061 T5058 pobj period,during
R4016 T5062 T5057 cc but,function
R4017 T5063 T5062 advmod rather,but
R4018 T5064 T5065 aux might,contribute
R4019 T5065 T5057 conj contribute,function
R4020 T5066 T5065 advmod later,contribute
R4021 T5067 T5066 prep to,later
R4022 T5068 T5069 det the,control
R4023 T5069 T5067 pobj control,to
R4024 T5070 T5069 prep of,control
R4025 T5071 T5072 compound perforin,expression
R4026 T5072 T5070 pobj expression,of
R4027 T5073 T5052 punct .,suggested
R4028 T5074 T5075 poss Our,data
R4029 T5075 T5076 nsubj data,seemed
R4030 T5076 T5106 ccomp seemed,induced
R4031 T5077 T5078 advmod most,consistent
R4032 T5078 T5076 oprd consistent,seemed
R4033 T5079 T5078 prep with,consistent
R4034 T5080 T5081 det a,model
R4035 T5081 T5079 pobj model,with
R4036 T5082 T5085 prep in,signals
R4037 T5083 T5082 pobj which,in
R4038 T5084 T5085 nsubj TCR,signals
R4039 T5085 T5086 nsubj signals,induce
R4040 T5086 T5076 ccomp induce,seemed
R4041 T5087 T5086 dobj T-bet,induce
R4042 T5088 T5087 punct ",",T-bet
R4043 T5089 T5092 nsubj which,induces
R4044 T5090 T5092 prep in,induces
R4045 T5091 T5090 pobj turn,in
R4046 T5092 T5087 relcl induces,T-bet
R4047 T5093 T5092 dobj IFN-γ,induces
R4048 T5094 T5093 punct (,IFN-γ
R4049 T5095 T5093 appos 17,IFN-γ
R4050 T5096 T5093 punct ),IFN-γ
R4051 T5097 T5092 cc and,induces
R4052 T5098 T5099 advmod possibly,granzyme
R4053 T5099 T5092 conj granzyme,induces
R4054 T5100 T5099 dobj B,granzyme
R4055 T5101 T5106 punct ;,induced
R4056 T5102 T5106 advmod subsequently,induced
R4057 T5103 T5106 punct ",",induced
R4058 T5104 T5106 nsubjpass Eomes,induced
R4059 T5105 T5106 auxpass is,induced
R4060 T5106 T5106 ROOT induced,induced
R4061 T5107 T5106 prep during,induced
R4804 T6120 T6125 nsubj Perforin,are
R4805 T6121 T6120 cc and,Perforin
R4806 T6122 T6123 compound granzyme,B
R4807 T6123 T6124 compound B,expression
R4808 T6124 T6120 conj expression,Perforin
R4809 T6125 T6128 auxpass are,regulated
R4810 T6126 T6128 neg not,regulated
R4811 T6127 T6128 advmod appreciably,regulated
R4812 T6128 T6143 advcl regulated,compared
R4813 T6129 T6128 agent by,regulated
R4814 T6130 T6129 pobj T-bet,by
R4815 T6131 T6132 aux To,test
R4816 T6132 T6128 xcomp test,regulated
R4817 T6133 T6134 det the,model
R4818 T6134 T6132 dobj model,test
R4819 T6135 T6134 acl outlined,model
R4820 T6136 T6135 prep in,outlined
R4821 T6137 T6139 det the,paragraph
R4822 T6138 T6139 amod previous,paragraph
R4823 T6139 T6136 pobj paragraph,in
R4824 T6140 T6135 advmod directly,outlined
R4825 T6141 T6143 punct ",",compared
R4826 T6142 T6143 nsubj we,compared
R4827 T6143 T6143 ROOT compared,compared
R4828 T6144 T6145 det the,expression
R4829 T6145 T6143 dobj expression,compared
R4830 T6146 T6145 prep of,expression
R4831 T6147 T6146 pobj IFN-γ,of
R4832 T6148 T6147 punct ",",IFN-γ
R4833 T6149 T6168 nmod perforin,mice
R4834 T6150 T6149 punct ",",perforin
R4835 T6151 T6149 cc and,perforin
R4836 T6152 T6153 compound granzyme,B
R4837 T6153 T6149 conj B,perforin
R4838 T6154 T6153 prep in,B
R4839 T6155 T6158 nummod CD8,cells
R4840 T6156 T6158 nummod +,cells
R4841 T6157 T6158 compound T,cells
R4842 T6158 T6154 pobj cells,in
R4843 T6159 T6153 prep from,B
R4844 T6160 T6159 pobj WT,from
R4845 T6161 T6160 cc and,WT
R4846 T6162 T6160 conj Tbx21,WT
R4847 T6163 T6164 punct (,T-bet
R4848 T6164 T6162 appos T-bet,Tbx21
R4849 T6165 T6164 punct ),T-bet
R4850 T6166 T6167 punct -,deficient
R4851 T6167 T6168 amod deficient,mice
R4852 T6168 T6147 appos mice,IFN-γ
R4853 T6169 T6143 punct .,compared
R4854 T6170 T6171 mark As,expected
R4855 T6171 T6187 advcl expected,produced
R4856 T6172 T6175 punct (,21
R4857 T6173 T6175 nummod 17,21
R4858 T6174 T6175 punct ",",21
R4859 T6175 T6171 parataxis 21,expected
R4860 T6176 T6175 punct ),21
R4861 T6177 T6187 punct ",",produced
R4862 T6178 T6186 amod naive,cells
R4863 T6179 T6186 nmod Tbx21,cells
R4864 T6180 T6186 nmod −,cells
R4865 T6181 T6186 nmod /,cells
R4866 T6182 T6186 nmod −,cells
R4867 T6183 T6186 nmod CD8,cells
R4868 T6184 T6186 nmod +,cells
R4869 T6185 T6186 compound T,cells
R4870 T6186 T6187 nsubj cells,produced
R4871 T6187 T6187 ROOT produced,produced
R4872 T6188 T6187 dobj IFN-γ,produced
R4873 T6189 T6190 advmod poorly,upon
R4874 T6190 T6187 prep upon,produced
R4950 T6266 T6264 pobj d,for
R4951 T6267 T6263 conj showed,cultured
R4952 T6268 T6269 det no,defect
R4953 T6269 T6267 dobj defect,showed
R4954 T6270 T6269 prep in,defect
R4955 T6271 T6273 amod perforin,expression
R4956 T6272 T6273 compound mRNA,expression
R4957 T6273 T6270 pobj expression,in
R4905 T6221 T6224 auxpass was,mitigated
R4906 T6222 T6223 advmod almost,completely
R4907 T6223 T6224 advmod completely,mitigated
R4908 T6224 T6208 conj mitigated,observed
R4909 T6225 T6224 prep by,mitigated
R4910 T6226 T6225 pobj day,by
R4911 T6227 T6226 nummod 6,day
R4912 T6228 T6229 punct (,Fig.
R4913 T6229 T6231 nmod Fig.,A
R4914 T6230 T6231 nummod 2,A
R4915 T6231 T6224 npadvmod A,mitigated
R4916 T6232 T6224 punct ),mitigated
R4917 T6233 T6208 punct .,observed
R4918 T6234 T6237 nsubj This,reflected
R4919 T6235 T6236 advmod most,likely
R4920 T6236 T6237 advmod likely,reflected
R4921 T6237 T6237 ROOT reflected,reflected
R4922 T6238 T6237 dobj compensation,reflected
R4923 T6239 T6237 prep by,reflected
R4924 T6240 T6239 pobj Eomes,by
R4925 T6241 T6240 punct ",",Eomes
R4926 T6242 T6243 nsubj which,was
R4927 T6243 T6245 auxpass was,induced
R4928 T6244 T6245 advmod strongly,induced
R4929 T6245 T6237 conj induced,reflected
R4930 T6246 T6245 prep between,induced
R4931 T6247 T6246 pobj days,between
R4932 T6248 T6247 nummod 4,days
R4933 T6249 T6247 cc and,days
R4934 T6250 T6247 conj 6,days
R4935 T6251 T6252 punct (,Fig.
R4936 T6252 T6247 conj Fig.,days
R4937 T6253 T6252 nummod 1,Fig.
R4938 T6254 T6252 punct ),Fig.
R4939 T6255 T6237 punct .,reflected
R4940 T6256 T6263 prep In,cultured
R4941 T6257 T6256 pobj contrast,In
R4942 T6258 T6263 punct ",",cultured
R4943 T6259 T6263 dep T-bet,cultured
R4944 T6260 T6262 amod deficient,cells
R4945 T6261 T6262 compound T,cells
R4946 T6262 T6263 nsubj cells,cultured
R4947 T6263 T6263 ROOT cultured,cultured
R4948 T6264 T6263 prep for,cultured
R4949 T6265 T6266 nummod 6,d
R5100 T6416 T6415 punct (,cells
R5101 T6417 T6419 nmod Fig.,B
R5102 T6418 T6419 nummod 2,B
R5103 T6419 T6415 appos B,cells
R5104 T6420 T6415 punct ",",cells
R5105 T6421 T6415 conj lanes,cells
R5106 T6422 T6415 appos 2,cells
R5107 T6423 T6422 punct ",",2
R5108 T6424 T6422 conj 3,2
R5109 T6425 T6424 punct ",",3
R5110 T6426 T6424 conj 5,3
R5111 T6427 T6415 punct ",",cells
R5112 T6428 T6415 cc and,cells
R5113 T6429 T6415 conj 6,cells
R5114 T6430 T6429 punct ),6
R5115 T6431 T6403 punct .,increased
R5116 T6432 T6433 mark As,expected
R5117 T6433 T6437 advcl expected,rescued
R5118 T6434 T6437 punct ",",rescued
R5119 T6435 T6437 nsubj Eo-VP16,rescued
R5120 T6436 T6437 advmod also,rescued
R5121 T6437 T6437 ROOT rescued,rescued
R5122 T6438 T6440 det the,defect
R5123 T6439 T6440 amod early,defect
R5124 T6440 T6437 dobj defect,rescued
R5125 T6441 T6440 prep in,defect
R5126 T6442 T6443 amod IFN-γ,production
R5127 T6443 T6441 pobj production,in
R4958 T6274 T6277 punct (,B
R4959 T6275 T6277 amod Fig.,B
R4960 T6276 T6277 nummod 2,B
R4961 T6277 T6273 appos B,expression
R4962 T6278 T6267 punct ",",showed
R4963 T6279 T6263 conj compare,cultured
R4964 T6280 T6279 dobj lanes,compare
R4965 T6281 T6280 nummod 1,lanes
R4966 T6282 T6280 cc and,lanes
R4967 T6283 T6280 conj 4,lanes
R4968 T6284 T6279 punct ),compare
R4969 T6285 T6263 punct .,cultured
R4970 T6286 T6288 nsubj We,observed
R4971 T6287 T6288 advmod consistently,observed
R4972 T6288 T6288 ROOT observed,observed
R4973 T6289 T6291 det a,reduction
R4974 T6290 T6291 amod modest,reduction
R4975 T6291 T6288 dobj reduction,observed
R4976 T6292 T6291 prep in,reduction
R4977 T6293 T6294 compound GzmB,mRNA
R4978 T6294 T6292 pobj mRNA,in
R4979 T6295 T6294 prep in,mRNA
R4980 T6296 T6295 pobj T-bet,in
R4981 T6297 T6299 amod deficient,cells
R4982 T6298 T6299 compound T,cells
R4983 T6299 T6305 nsubj cells,compare
R4984 T6300 T6299 punct (,cells
R4985 T6301 T6303 nmod Fig.,B
R4986 T6302 T6303 nummod 2,B
R4987 T6303 T6299 appos B,cells
R4988 T6304 T6299 punct ",",cells
R4989 T6305 T6288 ccomp compare,observed
R4990 T6306 T6305 dobj lanes,compare
R4991 T6307 T6306 nummod 1,lanes
R4992 T6308 T6306 cc and,lanes
R4993 T6309 T6306 conj 4,lanes
R4994 T6310 T6309 punct ),4
R4995 T6311 T6306 punct ",",lanes
R4996 T6312 T6315 nsubj which,translate
R4997 T6313 T6315 aux did,translate
R4998 T6314 T6315 neg not,translate
R4999 T6315 T6306 relcl translate,lanes
R5000 T6316 T6315 prep into,translate
R5001 T6317 T6318 det a,decrease
R5002 T6318 T6316 pobj decrease,into
R5003 T6319 T6318 prep in,decrease
R5004 T6320 T6319 pobj expression,in
R5005 T6321 T6320 prep of,expression
R5006 T6322 T6324 compound granzyme,protein
R5007 T6323 T6324 compound B,protein
R5008 T6324 T6321 pobj protein,of
R5009 T6325 T6326 punct (,Fig.
R5010 T6326 T6328 nmod Fig.,C
R5011 T6327 T6328 nummod 2,C
R5012 T6328 T6315 dobj C,translate
R5013 T6329 T6305 punct ),compare
R5014 T6330 T6288 punct .,observed
R5015 T6331 T6332 aux To,examine
R5016 T6332 T6339 advcl examine,transduced
R5017 T6333 T6334 det the,role
R5018 T6334 T6332 dobj role,examine
R5019 T6335 T6334 prep of,role
R5020 T6336 T6335 pobj Eomes,of
R5021 T6337 T6339 punct ",",transduced
R5022 T6338 T6339 nsubj we,transduced
R5023 T6339 T6339 ROOT transduced,transduced
R5024 T6340 T6344 amod naive,cells
R5025 T6341 T6344 nmod CD8,cells
R5026 T6342 T6344 nummod +,cells
R5027 T6343 T6344 compound T,cells
R5028 T6344 T6339 dobj cells,transduced
R5029 T6345 T6344 prep from,cells
R5030 T6346 T6345 pobj WT,from
R5031 T6347 T6346 cc and,WT
R5032 T6348 T6349 compound Tbx21,−
R5033 T6349 T6346 conj −,WT
R5034 T6350 T6339 conj /,transduced
R5035 T6351 T6352 nummod −,mice
R5036 T6352 T6350 dobj mice,/
R5037 T6353 T6350 prep with,/
R5038 T6354 T6353 pobj retroviruses,with
R5039 T6355 T6354 acl containing,retroviruses
R5040 T6356 T6359 amod internal,site
R5041 T6357 T6359 amod ribosome,site
R5042 T6358 T6359 compound entry,site
R5043 T6359 T6355 dobj site,containing
R5044 T6360 T6359 punct (,site
R5045 T6361 T6359 appos IRES,site
R5046 T6362 T6359 punct ),site
R5047 T6363 T6363 ROOT GFP,GFP
R5048 T6364 T6365 nsubj that,were
R5049 T6365 T6363 relcl were,GFP
R5050 T6366 T6367 preconj either,empty
R5051 T6367 T6365 acomp empty,were
R5052 T6368 T6367 cc or,empty
R5053 T6369 T6367 conj encoded,empty
R5054 T6370 T6373 det a,version
R5055 T6371 T6372 advmod strongly,transactivating
R5056 T6372 T6373 amod transactivating,version
R5057 T6373 T6369 dobj version,encoded
R5058 T6374 T6373 prep of,version
R5059 T6375 T6374 pobj Eomes,of
R5060 T6376 T6375 punct (,Eomes
R5061 T6377 T6375 appos Eo-VP16,Eomes
R5062 T6378 T6375 punct ),Eomes
R5063 T6379 T6375 punct (,Eomes
R5064 T6380 T6375 appos 8,Eomes
R5065 T6381 T6375 punct ),Eomes
R5066 T6382 T6369 punct ",",encoded
R5067 T6383 T6369 cc and,encoded
R5068 T6384 T6369 conj expanded,encoded
R5069 T6385 T6384 dobj them,expanded
R5070 T6386 T6384 prep for,expanded
R5071 T6387 T6386 pobj 6,for
R5072 T6388 T6384 advcl d,expanded
R5073 T6389 T6388 prep under,d
R5074 T6390 T6392 poss our,conditions
R5075 T6391 T6392 compound culture,conditions
R5076 T6392 T6389 pobj conditions,under
R5077 T6393 T6339 punct .,transduced
R5078 T6394 T6403 nsubj Eo-VP16,increased
R5079 T6395 T6394 punct ",",Eo-VP16
R5080 T6396 T6394 cc but,Eo-VP16
R5081 T6397 T6401 neg not,retrovirus
R5082 T6398 T6401 det the,retrovirus
R5083 T6399 T6401 amod empty,retrovirus
R5084 T6400 T6401 compound GFP,retrovirus
R5085 T6401 T6394 conj retrovirus,Eo-VP16
R5086 T6402 T6401 punct ",",retrovirus
R5087 T6403 T6403 ROOT increased,increased
R5088 T6404 T6405 compound perforin,expression
R5089 T6405 T6403 dobj expression,increased
R5090 T6406 T6403 prep in,increased
R5091 T6407 T6408 preconj both,WT
R5092 T6408 T6406 pobj WT,in
R5093 T6409 T6408 cc and,WT
R5094 T6410 T6408 conj T-bet,WT
R5095 T6411 T6415 amod deficient,cells
R5096 T6412 T6415 nmod CD8,cells
R5097 T6413 T6415 nummod +,cells
R5098 T6414 T6415 compound T,cells
R5099 T6415 T6403 advcl cells,increased
R5175 T6491 T6490 pobj Eo-VP16,by
R5176 T6492 T6488 punct .,compensated
R6174 T7997 T7998 amod Runx3,controls
R6175 T7998 T8044 nsubj controls,examined
R6176 T7999 T8000 amod multiple,aspects
R6177 T8000 T7998 dobj aspects,controls
R6178 T8001 T8000 prep of,aspects
R6179 T8002 T8005 det the,program
R6180 T8003 T8005 compound CTL,program
R6181 T8004 T8005 compound differentiation,program
R6182 T8005 T8001 pobj program,of
R6183 T8006 T7998 punct ",",controls
R6184 T8007 T7998 prep in,controls
R6185 T8008 T8007 pobj part,in
R6186 T8009 T7998 prep through,controls
R6187 T8010 T8009 pobj induction,through
R5128 T6444 T6437 conj observed,rescued
R5129 T6445 T6444 prep in,observed
R5130 T6446 T6445 pobj T-bet,in
R5131 T6447 T6451 amod deficient,cells
R5132 T6448 T6451 nmod CD8,cells
R5133 T6449 T6451 nummod +,cells
R5134 T6450 T6451 compound T,cells
R5135 T6451 T6444 dep cells,observed
R5136 T6452 T6451 punct (,cells
R5137 T6453 T6455 nmod Fig.,D
R5138 T6454 T6455 nummod 2,D
R5139 T6455 T6451 appos D,cells
R5140 T6456 T6451 punct ),cells
R5141 T6457 T6437 punct .,rescued
R5142 T6458 T6463 advmod However,induce
R5143 T6459 T6463 punct ",",induce
R5144 T6460 T6463 nsubj Eo-VP16,induce
R5145 T6461 T6463 aux did,induce
R5146 T6462 T6463 neg not,induce
R5147 T6463 T6463 ROOT induce,induce
R5148 T6464 T6465 compound GzmB,mRNA
R5149 T6465 T6466 compound mRNA,expression
R5150 T6466 T6463 dobj expression,induce
R5151 T6467 T6463 prep in,induce
R5152 T6468 T6469 preconj either,WT
R5153 T6469 T6467 pobj WT,in
R5154 T6470 T6469 cc or,WT
R5155 T6471 T6469 conj T-bet,WT
R5156 T6472 T6473 amod deficient,cells
R5157 T6473 T6463 dobj cells,induce
R5158 T6474 T6463 punct ;,induce
R5159 T6475 T6463 advmod thus,induce
R5160 T6476 T6488 punct ",",compensated
R5161 T6477 T6480 det the,dependence
R5162 T6478 T6480 amod partial,dependence
R5163 T6479 T6480 compound T-bet,dependence
R5164 T6480 T6488 nsubjpass dependence,compensated
R5165 T6481 T6480 prep of,dependence
R5166 T6482 T6483 compound GzmB,mRNA
R5167 T6483 T6484 compound mRNA,expression
R5168 T6484 T6481 pobj expression,of
R5169 T6485 T6488 aux can,compensated
R5170 T6486 T6488 neg not,compensated
R5171 T6487 T6488 auxpass be,compensated
R5172 T6488 T6488 ROOT compensated,compensated
R5173 T6489 T6488 prep for,compensated
R5174 T6490 T6488 agent by,compensated
R6338 T8161 T8157 prep at,/
R6339 T8162 T8161 pobj day,at
R6340 T8163 T8162 nummod 6,day
R6341 T8164 T8162 prep of,day
R6342 T8165 T8164 pobj culture,of
R6343 T8166 T8170 punct (,A
R6344 T8167 T8170 intj Fig.,A
R6345 T8168 T8167 nummod 3,Fig.
R6346 T8169 T8167 punct ",",Fig.
R6347 T8170 T8157 parataxis A,/
R6348 T8171 T8170 cc and,A
R6349 T8172 T8170 conj B,A
R6350 T8173 T8172 punct ),B
R6351 T8174 T8151 punct .,were
R6352 T8175 T8180 amod Runx3,cells
R6353 T8176 T8180 nmod −,cells
R6354 T8177 T8180 nmod /,cells
R6355 T8178 T8180 nmod −,cells
R6356 T8179 T8180 compound T,cells
R6357 T8180 T8182 nsubj cells,had
R6358 T8181 T8182 advmod also,had
R6359 T8182 T8194 ccomp had,unimpaired
R6360 T8183 T8186 det no,expression
R6361 T8184 T8186 amod detectable,expression
R6362 T8185 T8186 compound Eomes,expression
R6363 T8186 T8182 dobj expression,had
R6364 T8187 T8194 punct ;,unimpaired
R6188 T8011 T8010 prep of,induction
R6189 T8012 T8011 pobj Eomes,of
R6190 T8013 T8017 mark Because,expressed
R6191 T8014 T8017 nsubjpass Runx3,expressed
R6192 T8015 T8017 auxpass is,expressed
R6193 T8016 T8017 advmod highly,expressed
R6194 T8017 T7998 advcl expressed,controls
R6195 T8018 T8017 prep in,expressed
R6196 T8019 T8023 amod peripheral,cells
R6197 T8020 T8023 nmod CD8,cells
R6198 T8021 T8023 nummod +,cells
R6199 T8022 T8023 compound T,cells
R6200 T8023 T8018 pobj cells,in
R6201 T8024 T8017 punct ",",expressed
R6202 T8025 T8017 cc and,expressed
R6203 T8026 T8044 prep because,examined
R6204 T8027 T8026 pcomp of,because
R6205 T8028 T8030 det the,cooperation
R6206 T8029 T8030 amod T-bet-Runx3,cooperation
R6207 T8030 T8026 pobj cooperation,because
R6208 T8031 T8032 nsubj we,observed
R6209 T8032 T8030 relcl observed,cooperation
R6210 T8033 T8032 advmod earlier,observed
R6211 T8034 T8033 prep in,earlier
R6212 T8035 T8038 nummod CD4,cells
R6213 T8036 T8038 nummod +,cells
R6214 T8037 T8038 compound T,cells
R6215 T8038 T8034 pobj cells,in
R6216 T8039 T8030 punct (,cooperation
R6217 T8040 T8030 appos 15,cooperation
R6218 T8041 T8030 punct ),cooperation
R6219 T8042 T8044 punct ",",examined
R6220 T8043 T8044 nsubj we,examined
R6221 T8044 T8044 ROOT examined,examined
R6222 T8045 T8046 det the,role
R6223 T8046 T8044 dobj role,examined
R6224 T8047 T8046 prep of,role
R6225 T8048 T8047 pobj Runx3,of
R6226 T8049 T8046 prep in,role
R6227 T8050 T8051 compound effector,CTL
R6228 T8051 T8052 compound CTL,differentiation
R6229 T8052 T8049 pobj differentiation,in
R6230 T8053 T8044 punct .,examined
R6231 T8054 T8055 nsubj We,isolated
R6232 T8055 T8055 ROOT isolated,isolated
R6233 T8056 T8059 compound CD8,cells
R6234 T8057 T8059 compound +,cells
R6235 T8058 T8059 compound T,cells
R6236 T8059 T8055 dobj cells,isolated
R6237 T8060 T8059 prep from,cells
R6238 T8061 T8064 amod Runx3,−
R6239 T8062 T8064 compound −,−
R6240 T8063 T8064 compound /,−
R6241 T8064 T8060 pobj −,from
R6242 T8065 T8064 punct (,−
R6243 T8066 T8064 parataxis KO,−
R6244 T8067 T8064 punct ),−
R6245 T8068 T8060 pobj mice,from
R6246 T8069 T8068 prep of,mice
R6247 T8070 T8073 det the,background
R6248 T8071 T8073 amod outbred,background
R6249 T8072 T8073 compound ICR,background
R6250 T8073 T8069 pobj background,of
R6251 T8074 T8073 cc and,background
R6252 T8075 T8077 poss their,Runx3
R6253 T8076 T8077 compound WT,Runx3
R6254 T8077 T8078 nsubj Runx3,+
R6255 T8078 T8055 conj +,isolated
R6256 T8079 T8081 nummod /,littermates
R6257 T8080 T8081 compound +,littermates
R6258 T8081 T8078 dobj littermates,+
R6259 T8082 T8078 prep by,+
R6260 T8083 T8084 amod positive,selection
R6261 T8084 T8082 pobj selection,by
R6262 T8085 T8084 prep with,selection
R6263 T8086 T8088 amod anti-CD8,beads
R6264 T8087 T8088 amod magnetic,beads
R6265 T8088 T8085 pobj beads,with
R6266 T8089 T8088 punct (,beads
R6267 T8090 T8088 appos Figs,beads
R6268 T8091 T8078 punct .,+
R6269 T8092 T8092 ROOT S1,S1
R6270 T8093 T8092 cc and,S1
R6271 T8094 T8092 conj S2,S1
R6272 T8095 T8092 punct ",",S1
R6273 T8096 T8092 amod available,S1
R6274 T8097 T8096 prep at,available
R6275 T8098 T8097 pobj http://www.jem.org/cgi/content/full/jem.20081242/DC1,at
R6276 T8099 T8092 punct ),S1
R6277 T8100 T8092 punct .,S1
R6278 T8101 T8113 advmod Strikingly,impaired
R6279 T8102 T8113 punct ",",impaired
R6280 T8103 T8110 amod Runx3,cells
R6281 T8104 T8110 nmod −,cells
R6282 T8105 T8110 nmod /,cells
R6283 T8106 T8110 nmod −,cells
R6284 T8107 T8110 nmod CD8,cells
R6285 T8108 T8110 nmod +,cells
R6286 T8109 T8110 compound T,cells
R6287 T8110 T8113 nsubjpass cells,impaired
R6288 T8111 T8113 auxpass were,impaired
R6289 T8112 T8113 advmod strongly,impaired
R6290 T8113 T8113 ROOT impaired,impaired
R6291 T8114 T8113 prep in,impaired
R6292 T8115 T8116 poss their,ability
R6293 T8116 T8114 pobj ability,in
R6294 T8117 T8118 aux to,differentiate
R6295 T8118 T8116 acl differentiate,ability
R6296 T8119 T8118 prep into,differentiate
R6297 T8120 T8121 compound effector,CTLs
R6298 T8121 T8119 pobj CTLs,into
R6299 T8122 T8113 punct ",",impaired
R6300 T8123 T8124 mark as,judged
R6301 T8124 T8113 advcl judged,impaired
R6302 T8125 T8124 agent by,judged
R6303 T8126 T8125 pobj expression,by
R6304 T8127 T8126 prep of,expression
R6305 T8128 T8127 pobj perforin,of
R6306 T8129 T8113 punct ",",impaired
R6307 T8130 T8131 compound granzyme,B
R6308 T8131 T8113 dobj B,impaired
R6309 T8132 T8113 punct ",",impaired
R6310 T8133 T8113 cc and,impaired
R6311 T8134 T8113 conj IFN-γ,impaired
R6312 T8135 T8136 punct (,Fig.
R6313 T8136 T8134 appos Fig.,IFN-γ
R6314 T8137 T8136 nummod 3,Fig.
R6315 T8138 T8136 punct ),Fig.
R6316 T8139 T8113 punct .,impaired
R6317 T8140 T8151 prep Compared,were
R6318 T8141 T8140 prep with,Compared
R6319 T8142 T8144 compound WT,cells
R6320 T8143 T8144 compound T,cells
R6321 T8144 T8141 pobj cells,with
R6322 T8145 T8151 punct ",",were
R6323 T8146 T8147 compound perforin,mRNA
R6324 T8147 T8151 nsubj mRNA,were
R6325 T8148 T8147 cc and,mRNA
R6326 T8149 T8150 compound protein,expression
R6327 T8150 T8147 conj expression,mRNA
R6328 T8151 T8151 ROOT were,were
R6329 T8152 T8153 advmod essentially,undetectable
R6330 T8153 T8151 acomp undetectable,were
R6331 T8154 T8153 prep in,undetectable
R6332 T8155 T8156 compound Runx3,−
R6333 T8156 T8154 pobj −,in
R6334 T8157 T8151 conj /,were
R6335 T8158 T8160 nummod −,cells
R6336 T8159 T8160 compound T,cells
R6337 T8160 T8157 dobj cells,/
R6413 T8236 T8241 mark that,regulated
R6414 T8237 T8239 amod Th1,differentiation
R6415 T8238 T8239 compound cell,differentiation
R6416 T8239 T8241 nsubjpass differentiation,regulated
R6417 T8240 T8241 auxpass was,regulated
R6418 T8241 T8235 ccomp regulated,reported
R6419 T8242 T8241 prep through,regulated
R6420 T8243 T8245 det a,loop
R6365 T8188 T8194 prep in,unimpaired
R6366 T8189 T8188 pobj contrast,in
R6367 T8190 T8194 punct ",",unimpaired
R6368 T8191 T8192 compound T-bet,expression
R6369 T8192 T8194 nsubjpass expression,unimpaired
R6370 T8193 T8194 auxpass was,unimpaired
R6371 T8194 T8194 ROOT unimpaired,unimpaired
R6372 T8195 T8194 punct (,unimpaired
R6373 T8196 T8194 parataxis Fig.,unimpaired
R6374 T8197 T8198 nummod 3,A
R6375 T8198 T8196 pobj A,Fig.
R6376 T8199 T8194 punct ),unimpaired
R6377 T8200 T8194 punct .,unimpaired
R6378 T8201 T8205 advmod Furthermore,required
R6379 T8202 T8205 punct ",",required
R6380 T8203 T8205 nsubjpass Runx3,required
R6381 T8204 T8205 auxpass was,required
R6382 T8205 T8205 ROOT required,required
R6383 T8206 T8205 prep for,required
R6384 T8207 T8208 amod maximal,production
R6385 T8208 T8206 pobj production,for
R6386 T8209 T8208 prep of,production
R6387 T8210 T8209 pobj IFN-γ,of
R6388 T8211 T8205 punct ",",required
R6389 T8212 T8205 cc but,required
R6390 T8213 T8214 neg not,TNF
R6391 T8214 T8214 ROOT TNF,TNF
R6392 T8215 T8214 cc or,TNF
R6393 T8216 T8214 conj IL-2,TNF
R6394 T8217 T8214 punct ",",TNF
R6395 T8218 T8223 agent by,restimulated
R6396 T8219 T8222 nummod CD8,cells
R6397 T8220 T8222 nummod +,cells
R6398 T8221 T8222 compound T,cells
R6399 T8222 T8218 pobj cells,by
R6400 T8223 T8205 conj restimulated,required
R6401 T8224 T8223 prep at,restimulated
R6402 T8225 T8224 pobj day,at
R6403 T8226 T8225 nummod 6,day
R6404 T8227 T8228 punct (,Fig.
R6405 T8228 T8223 parataxis Fig.,restimulated
R6406 T8229 T8228 nummod 3,Fig.
R6407 T8230 T8228 appos C,Fig.
R6408 T8231 T8228 punct ),Fig.
R6409 T8232 T8205 punct .,required
R6410 T8233 T8235 nsubj We,reported
R6411 T8234 T8235 advmod previously,reported
R6412 T8235 T8235 ROOT reported,reported
R6563 T8386 T8385 prep for,necessary
R6564 T8387 T8388 compound Eomes,induction
R6565 T8388 T8386 pobj induction,for
R6566 T8389 T8391 punct ",",cooperated
R6567 T8390 T8391 advmod then,cooperated
R6568 T8391 T8379 ccomp cooperated,asked
R6569 T8392 T8391 prep with,cooperated
R6570 T8393 T8392 pobj Eomes,with
R6571 T8394 T8395 aux to,regulate
R6572 T8395 T8391 advcl regulate,cooperated
R6573 T8396 T8395 dobj transcription,regulate
R6574 T8397 T8396 prep of,transcription
R6575 T8398 T8401 det the,markers
R6576 T8399 T8400 compound effector,CTL
R6577 T8400 T8401 compound CTL,markers
R6578 T8401 T8397 pobj markers,of
R6579 T8402 T8404 nmod perforin,IFN-γ
R6580 T8403 T8402 punct ",",perforin
R6581 T8404 T8395 dobj IFN-γ,regulate
R6582 T8405 T8404 punct ",",IFN-γ
R6583 T8406 T8404 cc and,IFN-γ
R6584 T8407 T8408 compound granzyme,B.
R6585 T8408 T8410 nsubj B.,test
R6586 T8409 T8410 aux To,test
R6587 T8410 T8391 advcl test,cooperated
R6588 T8411 T8412 det this,hypothesis
R6589 T8412 T8410 dobj hypothesis,test
R6590 T8413 T8415 punct ",",used
R6421 T8244 T8245 amod feed-forward,loop
R6422 T8245 T8242 pobj loop,through
R6423 T8246 T8249 prep in,is
R6424 T8247 T8246 pobj which,in
R6425 T8248 T8249 nsubj T-bet,is
R6426 T8249 T8245 relcl is,loop
R6427 T8250 T8249 acomp up-regulated,is
R6428 T8251 T8249 advmod early,is
R6429 T8252 T8249 cc and,is
R6430 T8253 T8249 conj induces,is
R6431 T8254 T8253 dobj Runx3,induces
R6432 T8255 T8253 punct ",",induces
R6433 T8256 T8261 prep after,cooperate
R6434 T8257 T8256 pobj which,after
R6435 T8258 T8261 nsubj T-bet,cooperate
R6436 T8259 T8258 cc and,T-bet
R6437 T8260 T8258 conj Runx3,T-bet
R6438 T8261 T8241 advcl cooperate,regulated
R6439 T8262 T8263 aux to,induce
R6440 T8263 T8261 xcomp induce,cooperate
R6441 T8264 T8263 dobj IFN-γ,induce
R6442 T8265 T8264 cc and,IFN-γ
R6443 T8266 T8267 compound silence,IL-4
R6444 T8267 T8264 conj IL-4,IFN-γ
R6445 T8268 T8263 punct ",",induce
R6446 T8269 T8270 advmod thus,promoting
R6447 T8270 T8261 advcl promoting,cooperate
R6448 T8271 T8272 amod stable,differentiation
R6449 T8272 T8270 dobj differentiation,promoting
R6450 T8273 T8272 prep toward,differentiation
R6451 T8274 T8276 det the,lineage
R6452 T8275 T8276 amod Th1,lineage
R6453 T8276 T8273 pobj lineage,toward
R6454 T8277 T8276 punct (,lineage
R6455 T8278 T8280 quantmod 15,22
R6456 T8279 T8280 punct ",",22
R6457 T8280 T8276 appos 22,lineage
R6458 T8281 T8276 punct ),lineage
R6459 T8282 T8235 punct .,reported
R6460 T8283 T8288 mark Because,appeared
R6461 T8284 T8285 punct (,a
R6462 T8285 T8288 meta a,appeared
R6463 T8286 T8285 punct ),a
R6464 T8287 T8288 nsubj Runx3,appeared
R6465 T8288 T8288 ROOT appeared,appeared
R6466 T8289 T8288 oprd necessary,appeared
R6467 T8290 T8288 prep for,appeared
R6468 T8291 T8292 compound Eomes,induction
R6469 T8292 T8290 pobj induction,for
R6470 T8293 T8294 punct (,Fig.
R6471 T8294 T8296 nmod Fig.,A
R6472 T8295 T8296 nummod 3,A
R6473 T8296 T8292 appos A,induction
R6474 T8297 T8296 punct ),A
R6475 T8298 T8292 punct ",",induction
R6476 T8299 T8300 punct (,b
R6477 T8300 T8292 appos b,induction
R6478 T8301 T8300 punct ),b
R6479 T8302 T8303 det the,kinetics
R6480 T8303 T8307 nsubj kinetics,paralleled
R6481 T8304 T8303 prep of,kinetics
R6482 T8305 T8306 compound Eomes,expression
R6483 T8306 T8304 pobj expression,of
R6484 T8307 T8288 conj paralleled,appeared
R6485 T8308 T8307 dobj those,paralleled
R6486 T8309 T8308 prep of,those
R6487 T8310 T8311 compound perforin,expression
R6488 T8311 T8309 pobj expression,of
R6489 T8312 T8311 punct (,expression
R6490 T8313 T8311 appos Fig.,expression
R6491 T8314 T8313 pobj 2,Fig.
R6492 T8315 T8311 punct ),expression
R6493 T8316 T8307 punct ",",paralleled
R6494 T8317 T8307 cc and,paralleled
R6495 T8318 T8319 punct (,c
R6496 T8319 T8321 meta c,overexpression
R6497 T8320 T8319 punct ),c
R6498 T8321 T8332 npadvmod overexpression,led
R6499 T8322 T8321 prep of,overexpression
R6500 T8323 T8322 pobj Eo-VP16,of
R6501 T8324 T8321 prep in,overexpression
R6502 T8325 T8326 det either,WT
R6503 T8326 T8324 pobj WT,in
R6504 T8327 T8326 cc or,WT
R6505 T8328 T8326 conj T-bet,WT
R6506 T8329 T8331 amod deficient,cells
R6507 T8330 T8331 compound T,cells
R6508 T8331 T8332 nsubj cells,led
R6509 T8332 T8307 conj led,paralleled
R6510 T8333 T8332 prep to,led
R6511 T8334 T8335 det an,increase
R6512 T8335 T8333 pobj increase,to
R6513 T8336 T8335 prep in,increase
R6514 T8337 T8338 preconj both,perforin
R6515 T8338 T8336 pobj perforin,in
R6516 T8339 T8338 cc and,perforin
R6517 T8340 T8341 amod IFN-γ,expression
R6518 T8341 T8338 conj expression,perforin
R6519 T8342 T8352 punct (,asked
R6520 T8343 T8352 nsubj Fig.,asked
R6521 T8344 T8343 nummod 2,Fig.
R6522 T8345 T8352 punct ",",asked
R6523 T8346 T8352 advcl B,asked
R6524 T8347 T8346 cc and,B
R6525 T8348 T8346 conj D,B
R6526 T8349 T8346 punct ),B
R6527 T8350 T8352 punct ",",asked
R6528 T8351 T8352 nsubj we,asked
R6529 T8352 T8352 ROOT asked,asked
R6530 T8353 T8356 mark whether,was
R6531 T8354 T8355 compound CTL,differentiation
R6532 T8355 T8356 nsubj differentiation,was
R6533 T8356 T8359 auxpass was,regulated
R6534 T8357 T8359 advmod also,regulated
R6535 T8358 T8359 advmod potentially,regulated
R6536 T8359 T8352 ccomp regulated,asked
R6537 T8360 T8359 agent by,regulated
R6538 T8361 T8363 det a,loop
R6539 T8362 T8363 amod feed-forward,loop
R6540 T8363 T8360 pobj loop,by
R6541 T8364 T8363 acl involving,loop
R6542 T8365 T8368 det these,classes
R6543 T8366 T8368 amod same,classes
R6544 T8367 T8368 nummod two,classes
R6545 T8368 T8364 dobj classes,involving
R6546 T8369 T8368 prep of,classes
R6547 T8370 T8369 pobj Runx,of
R6548 T8371 T8370 cc and,Runx
R6549 T8372 T8370 conj T-box,Runx
R6550 T8373 T8374 compound transcription,factors
R6551 T8374 T8368 conj factors,classes
R6552 T8375 T8352 punct .,asked
R6553 T8376 T8379 advmod Specifically,asked
R6554 T8377 T8379 punct ",",asked
R6555 T8378 T8379 nsubj we,asked
R6556 T8379 T8379 ROOT asked,asked
R6557 T8380 T8391 mark whether,cooperated
R6558 T8381 T8391 nsubj Runx3,cooperated
R6559 T8382 T8381 punct ",",Runx3
R6560 T8383 T8384 nsubj which,was
R6561 T8384 T8381 relcl was,Runx3
R6562 T8385 T8384 acomp necessary,was
R6638 T8461 T8459 pobj Prf1,to
R6639 T8462 T8461 cc and,Prf1
R6640 T8463 T8461 conj Gzmb,Prf1
R6641 T8464 T8465 advmod transcription,start
R6642 T8465 T8470 preconj start,;
R6643 T8466 T8465 dobj sites,start
R6644 T8467 T8468 punct (,TSS
R6645 T8468 T8466 appos TSS,sites
R6646 T8469 T8466 punct ),sites
R6647 T8470 T8458 punct ;,bound
R6648 T8471 T8517 ccomp to,reported
R6649 T8472 T8474 det a,IL-2
R6650 T8473 T8474 amod known,IL-2
R6651 T8474 T8476 nmod IL-2,enhancer
R6652 T8475 T8476 amod responsive,enhancer
R6653 T8476 T8471 pobj enhancer,to
R6591 T8414 T8415 nsubj we,used
R6592 T8415 T8415 ROOT used,used
R6593 T8416 T8417 compound chromatin,immunoprecipitation
R6594 T8417 T8415 dobj immunoprecipitation,used
R6595 T8418 T8419 punct (,ChIP
R6596 T8419 T8417 appos ChIP,immunoprecipitation
R6597 T8420 T8417 punct ),immunoprecipitation
R6598 T8421 T8415 dobj assays,used
R6599 T8422 T8423 aux to,ask
R6600 T8423 T8415 xcomp ask,used
R6601 T8424 T8428 mark whether,bound
R6602 T8425 T8428 nsubj Eomes,bound
R6603 T8426 T8425 cc and,Eomes
R6604 T8427 T8425 conj Runx3,Eomes
R6605 T8428 T8423 ccomp bound,ask
R6606 T8429 T8430 amod regulatory,regions
R6607 T8430 T8428 dobj regions,bound
R6608 T8431 T8430 prep of,regions
R6609 T8432 T8433 det the,Prf1
R6610 T8433 T8439 nmod Prf1,genes
R6611 T8434 T8433 punct ",",Prf1
R6612 T8435 T8433 conj Ifng,Prf1
R6613 T8436 T8435 punct ",",Ifng
R6614 T8437 T8435 cc and,Ifng
R6615 T8438 T8435 conj Gzmb,Ifng
R6616 T8439 T8431 pobj genes,of
R6617 T8440 T8443 punct (,D
R6618 T8441 T8443 nmod Fig.,D
R6619 T8442 T8441 nummod 3,Fig.
R6620 T8443 T8439 appos D,genes
R6621 T8444 T8423 punct ),ask
R6622 T8445 T8415 punct .,used
R6623 T8446 T8447 det Both,proteins
R6624 T8447 T8447 ROOT proteins,proteins
R6625 T8448 T8447 acl associated,proteins
R6626 T8449 T8448 prep with,associated
R6627 T8450 T8452 amod gene,regions
R6628 T8451 T8452 amod regulatory,regions
R6629 T8452 T8449 pobj regions,with
R6630 T8453 T8452 prep in,regions
R6631 T8454 T8455 amod differentiated,CTLs
R6632 T8455 T8453 pobj CTLs,in
R6633 T8456 T8447 punct .,proteins
R6634 T8457 T8458 amod Runx3,bound
R6635 T8458 T8517 ccomp bound,reported
R6636 T8459 T8458 prep to,bound
R6637 T8460 T8461 det the,Prf1
R6788 T8611 T8606 conj cells,Th1
R6789 T8612 T8613 punct (,Fig.
R6790 T8613 T8615 nmod Fig.,D
R6791 T8614 T8613 nummod 3,Fig.
R6792 T8615 T8600 npadvmod D,target
R6793 T8616 T8596 punct ),TSS
R6794 T8617 T8596 punct (,TSS
R6795 T8618 T8596 appos 17,TSS
R6796 T8619 T8596 punct ),TSS
R6797 T8620 T8564 punct .,was
R6798 T8621 T8622 aux To,determine
R6799 T8622 T8640 advcl determine,expressed
R6800 T8623 T8625 mark whether,controlled
R6801 T8624 T8625 nsubj Runx3,controlled
R6802 T8625 T8622 ccomp controlled,determine
R6803 T8626 T8627 det the,expression
R6804 T8627 T8625 dobj expression,controlled
R6805 T8628 T8627 prep of,expression
R6806 T8629 T8631 nmod CTL,genes
R6807 T8630 T8631 compound effector,genes
R6808 T8631 T8628 pobj genes,of
R6809 T8632 T8625 prep through,controlled
R6810 T8633 T8634 poss its,induction
R6811 T8634 T8632 pobj induction,through
R6812 T8635 T8634 prep of,induction
R6813 T8636 T8635 pobj Eomes,of
R6814 T8637 T8640 punct ",",expressed
R6815 T8638 T8640 nsubj we,expressed
R6816 T8639 T8640 advmod retrovirally,expressed
R6654 T8477 T8476 acl located,enhancer
R6655 T8478 T8477 prep near,located
R6656 T8479 T8481 nummod −,kb
R6657 T8480 T8481 nummod 1,kb
R6658 T8481 T8478 pobj kb,near
R6659 T8482 T8481 prep of,kb
R6660 T8483 T8485 det the,gene
R6661 T8484 T8485 compound Prf1,gene
R6662 T8485 T8482 pobj gene,of
R6663 T8486 T8485 punct (,gene
R6664 T8487 T8485 appos 23,gene
R6665 T8488 T8485 punct ),gene
R6666 T8489 T8471 punct ;,to
R6667 T8490 T8496 prep to,hypersensitive
R6668 T8491 T8494 det the,DNase
R6669 T8492 T8494 amod distal,DNase
R6670 T8493 T8494 compound CTL-specific,DNase
R6671 T8494 T8490 pobj DNase,to
R6672 T8495 T8496 nsubj I,hypersensitive
R6673 T8496 T8517 ccomp hypersensitive,reported
R6674 T8497 T8496 dobj site,hypersensitive
R6675 T8498 T8497 nummod 9,site
R6676 T8499 T8496 prep in,hypersensitive
R6677 T8500 T8502 det the,locus
R6678 T8501 T8502 amod Prf1,locus
R6679 T8502 T8499 pobj locus,in
R6680 T8503 T8502 punct (,locus
R6681 T8504 T8502 appos 24,locus
R6682 T8505 T8502 punct ),locus
R6683 T8506 T8517 punct ;,reported
R6684 T8507 T8517 prep to,reported
R6685 T8508 T8510 det the,promoter
R6686 T8509 T8510 compound Ifng,promoter
R6687 T8510 T8507 pobj promoter,to
R6688 T8511 T8510 prep near,promoter
R6689 T8512 T8513 det the,TSS
R6690 T8513 T8511 pobj TSS,near
R6691 T8514 T8517 punct ",",reported
R6692 T8515 T8517 mark as,reported
R6693 T8516 T8517 advmod previously,reported
R6694 T8517 T8517 ROOT reported,reported
R6695 T8518 T8517 prep for,reported
R6696 T8519 T8520 amod Th1,cells
R6697 T8520 T8518 pobj cells,for
R6698 T8521 T8522 punct (,10
R6699 T8522 T8517 npadvmod 10,reported
R6700 T8523 T8522 punct ),10
R6701 T8524 T8517 punct ;,reported
R6702 T8525 T8517 cc and,reported
R6703 T8526 T8530 prep to,hypersensitive
R6704 T8527 T8528 amod several,DNase
R6705 T8528 T8526 pobj DNase,to
R6706 T8529 T8530 nsubj I,hypersensitive
R6707 T8530 T8530 ROOT hypersensitive,hypersensitive
R6708 T8531 T8530 dobj sites,hypersensitive
R6709 T8532 T8531 prep in,sites
R6710 T8533 T8535 det the,locus
R6711 T8534 T8535 compound Ifng,locus
R6712 T8535 T8532 pobj locus,in
R6713 T8536 T8537 punct (,Fig.
R6714 T8537 T8539 nmod Fig.,D
R6715 T8538 T8539 nummod 3,D
R6716 T8539 T8530 npadvmod D,hypersensitive
R6717 T8540 T8530 cc and,hypersensitive
R6718 T8541 T8542 neg not,depicted
R6719 T8542 T8530 conj depicted,hypersensitive
R6720 T8543 T8542 punct ),depicted
R6721 T8544 T8545 punct (,25
R6722 T8545 T8530 appos 25,hypersensitive
R6723 T8546 T8530 punct ),hypersensitive
R6724 T8547 T8517 punct .,reported
R6725 T8548 T8564 nsubj Eomes,was
R6726 T8549 T8548 acl bound,Eomes
R6727 T8550 T8549 advmod primarily,bound
R6728 T8551 T8549 prep to,bound
R6729 T8552 T8554 det the,TSS
R6730 T8553 T8554 compound Prf1,TSS
R6731 T8554 T8551 pobj TSS,to
R6732 T8555 T8554 cc and,TSS
R6733 T8556 T8560 det the,enhancer
R6734 T8557 T8560 nmod −,enhancer
R6735 T8558 T8559 nummod 1,kb
R6736 T8559 T8560 compound kb,enhancer
R6737 T8560 T8554 conj enhancer,TSS
R6738 T8561 T8564 punct ;,was
R6739 T8562 T8563 det this,binding
R6740 T8563 T8564 nsubj binding,was
R6741 T8564 T8564 ROOT was,was
R6742 T8565 T8566 advmod substantially,greater
R6743 T8566 T8564 acomp greater,was
R6744 T8567 T8566 prep than,greater
R6745 T8568 T8567 pobj that,than
R6746 T8569 T8568 acl observed,that
R6747 T8570 T8569 prep at,observed
R6748 T8571 T8572 det the,promoter
R6749 T8572 T8570 pobj promoter,at
R6750 T8573 T8572 prep of,promoter
R6751 T8574 T8576 det the,gene
R6752 T8575 T8576 amod Il2rb,gene
R6753 T8576 T8573 pobj gene,of
R6754 T8577 T8576 punct ",",gene
R6755 T8578 T8581 det a,target
R6756 T8579 T8581 amod known,target
R6757 T8580 T8581 amod direct,target
R6758 T8581 T8576 appos target,gene
R6759 T8582 T8581 prep of,target
R6760 T8583 T8582 pobj Eomes,of
R6761 T8584 T8583 punct (,Eomes
R6762 T8585 T8583 appos 8,Eomes
R6763 T8586 T8583 punct ),Eomes
R6764 T8587 T8581 punct ",",target
R6765 T8588 T8581 cc and,target
R6766 T8589 T8581 conj comparable,target
R6767 T8590 T8589 prep to,comparable
R6768 T8591 T8590 pobj that,to
R6769 T8592 T8591 acl observed,that
R6770 T8593 T8592 prep at,observed
R6771 T8594 T8596 det the,TSS
R6772 T8595 T8596 compound Ifng,TSS
R6773 T8596 T8593 pobj TSS,at
R6774 T8597 T8596 punct ",",TSS
R6775 T8598 T8600 det a,target
R6776 T8599 T8600 amod known,target
R6777 T8600 T8596 appos target,TSS
R6778 T8601 T8600 prep of,target
R6779 T8602 T8603 compound T-box,proteins
R6780 T8603 T8601 pobj proteins,of
R6781 T8604 T8600 prep in,target
R6782 T8605 T8606 det both,Th1
R6783 T8606 T8604 pobj Th1,in
R6784 T8607 T8606 cc and,Th1
R6785 T8608 T8611 nummod CD8,cells
R6786 T8609 T8611 nummod +,cells
R6787 T8610 T8611 compound T,cells
R6863 T8686 T8685 cc and,SP
R6864 T8687 T8685 conj CD8,SP
R6865 T8688 T8692 compound +,cells
R6866 T8689 T8692 compound CD4,cells
R6867 T8690 T8692 compound +,cells
R6868 T8691 T8692 compound DP,cells
R6869 T8692 T8676 pobj cells,between
R6870 T8693 T8675 prep in,difference
R6871 T8694 T8696 poss our,experiments
R6872 T8695 T8696 amod previous,experiments
R6873 T8696 T8693 pobj experiments,in
R6874 T8697 T8699 punct ",",used
R6875 T8698 T8699 nsubj we,used
R6876 T8699 T8699 ROOT used,used
R6877 T8700 T8708 amod total,cells
R6878 T8701 T8702 amod Runx3,−
R6879 T8702 T8708 amod −,cells
R6880 T8703 T8708 amod /,cells
R6881 T8704 T8708 amod −,cells
R6882 T8705 T8708 amod CD8,cells
R6883 T8706 T8708 amod +,cells
R6884 T8707 T8708 compound T,cells
R6885 T8708 T8699 dobj cells,used
R6886 T8709 T8699 prep without,used
R6817 T8640 T8640 ROOT expressed,expressed
R6818 T8641 T8640 dobj Runx3,expressed
R6819 T8642 T8641 cc and,Runx3
R6820 T8643 T8641 conj Eo-VP16,Runx3
R6821 T8644 T8640 prep in,expressed
R6822 T8645 T8648 compound CD8,cells
R6823 T8646 T8648 compound +,cells
R6824 T8647 T8648 compound T,cells
R6825 T8648 T8644 pobj cells,in
R6826 T8649 T8648 prep from,cells
R6827 T8650 T8654 amod Runx3,mice
R6828 T8651 T8654 compound −,mice
R6829 T8652 T8654 compound /,mice
R6830 T8653 T8654 compound −,mice
R6831 T8654 T8649 pobj mice,from
R6832 T8655 T8640 punct .,expressed
R6833 T8656 T8699 prep Because,used
R6834 T8657 T8656 pcomp of,Because
R6835 T8658 T8660 det the,number
R6836 T8659 T8660 amod limited,number
R6837 T8660 T8656 pobj number,Because
R6838 T8661 T8660 prep of,number
R6839 T8662 T8665 nummod CD8,cells
R6840 T8663 T8665 nummod +,cells
R6841 T8664 T8665 compound T,cells
R6842 T8665 T8661 pobj cells,of
R6843 T8666 T8660 prep in,number
R6844 T8667 T8668 det these,mice
R6845 T8668 T8666 pobj mice,in
R6846 T8669 T8660 punct ",",number
R6847 T8670 T8656 cc and,Because
R6848 T8671 T8673 mark because,saw
R6849 T8672 T8673 nsubj we,saw
R6850 T8673 T8699 advcl saw,used
R6851 T8674 T8675 det no,difference
R6852 T8675 T8673 dobj difference,saw
R6853 T8676 T8675 prep between,difference
R6854 T8677 T8685 nmod Runx3,SP
R6855 T8678 T8685 nmod −,SP
R6856 T8679 T8685 nmod /,SP
R6857 T8680 T8685 nmod −,SP
R6858 T8681 T8685 nmod CD8,SP
R6859 T8682 T8685 nmod +,SP
R6860 T8683 T8685 nmod CD4,SP
R6861 T8684 T8685 nmod −,SP
R6862 T8685 T8692 nmod SP,cells
R7013 T8836 T8835 cc and,Runx3
R7014 T8837 T8835 conj Eomes,Runx3
R7015 T8838 T8829 punct .,suggests
R7016 T8839 T8840 mark As,expected
R7017 T8840 T8859 advcl expected,showed
R7018 T8841 T8840 prep from,expected
R7019 T8842 T8843 poss their,defect
R7020 T8843 T8841 pobj defect,from
R7021 T8844 T8843 prep in,defect
R7022 T8845 T8844 pobj perforin,in
R7023 T8846 T8845 cc and,perforin
R7024 T8847 T8849 compound granzyme,expression
R7025 T8848 T8849 compound B,expression
R7026 T8849 T8845 conj expression,perforin
R7027 T8850 T8843 punct ",",defect
R7028 T8851 T8858 amod Runx3,cells
R7029 T8852 T8858 nmod −,cells
R7030 T8853 T8858 nmod /,cells
R7031 T8854 T8858 nmod −,cells
R7032 T8855 T8858 nmod CD8,cells
R7033 T8856 T8858 nmod +,cells
R7034 T8857 T8858 compound T,cells
R7035 T8858 T8859 nsubj cells,showed
R7036 T8859 T8859 ROOT showed,showed
R7037 T8860 T8862 amod defective,activity
R7038 T8861 T8862 compound cytolytic,activity
R7039 T8862 T8859 dobj activity,showed
R7040 T8863 T8859 prep in,showed
R6887 T8710 T8711 amod further,fractionation
R6888 T8711 T8709 pobj fractionation,without
R6889 T8712 T8699 prep as,used
R6890 T8713 T8712 pobj recipients,as
R6891 T8714 T8713 prep for,recipients
R6892 T8715 T8716 amod retroviral,transduction
R6893 T8716 T8714 pobj transduction,for
R6894 T8717 T8699 punct .,used
R6895 T8718 T8718 ROOT Reconstitution,Reconstitution
R6896 T8719 T8718 prep of,Reconstitution
R6897 T8720 T8727 nmod Runx3,cells
R6898 T8721 T8727 nmod −,cells
R6899 T8722 T8727 nmod /,cells
R6900 T8723 T8727 nmod −,cells
R6901 T8724 T8727 nmod CD8,cells
R6902 T8725 T8727 nmod +,cells
R6903 T8726 T8727 compound T,cells
R6904 T8727 T8719 pobj cells,of
R6905 T8728 T8727 prep with,cells
R6906 T8729 T8731 amod Runx3,expression
R6907 T8730 T8731 amod restored,expression
R6908 T8731 T8728 pobj expression,with
R6909 T8732 T8731 prep of,expression
R6910 T8733 T8732 pobj Eomes,of
R6911 T8734 T8736 advmod as,as
R6912 T8735 T8736 advmod well,as
R6913 T8736 T8731 cc as,expression
R6914 T8737 T8731 conj perforin,expression
R6915 T8738 T8718 punct ",",Reconstitution
R6916 T8739 T8740 compound granzyme,B
R6917 T8740 T8718 appos B,Reconstitution
R6918 T8741 T8718 punct ",",Reconstitution
R6919 T8742 T8718 cc and,Reconstitution
R6920 T8743 T8718 conj IFN-γ,Reconstitution
R6921 T8744 T8745 punct (,Fig.
R6922 T8745 T8743 appos Fig.,IFN-γ
R6923 T8746 T8745 nummod 4,Fig.
R6924 T8747 T8745 punct ",",Fig.
R6925 T8748 T8743 det A,IFN-γ
R6926 T8749 T8748 cc and,A
R6927 T8750 T8748 conj B,A
R6928 T8751 T8750 punct ),B
R6929 T8752 T8718 punct .,Reconstitution
R6930 T8753 T8757 prep In,−
R6931 T8754 T8753 pobj addition,In
R6932 T8755 T8757 punct ",",−
R6933 T8756 T8757 nsubj Runx3,−
R6934 T8757 T8757 ROOT −,−
R6935 T8758 T8761 nummod /,cells
R6936 T8759 T8761 nummod −,cells
R6937 T8760 T8761 compound T,cells
R6938 T8761 T8762 nsubj cells,showed
R6939 T8762 T8757 ccomp showed,−
R6940 T8763 T8765 det a,up-regulation
R6941 T8764 T8765 amod compensatory,up-regulation
R6942 T8765 T8762 dobj up-regulation,showed
R6943 T8766 T8765 prep of,up-regulation
R6944 T8767 T8766 pobj Runx1,of
R6945 T8768 T8767 punct ",",Runx1
R6946 T8769 T8771 nsubjpass which,suppressed
R6947 T8770 T8771 auxpass was,suppressed
R6948 T8771 T8767 relcl suppressed,Runx1
R6949 T8772 T8771 prep upon,suppressed
R6950 T8773 T8772 pobj reconstitution,upon
R6951 T8774 T8771 prep with,suppressed
R6952 T8775 T8774 pobj Runx3,with
R6953 T8776 T8762 punct ",",showed
R6954 T8777 T8762 advcl indicating,showed
R6955 T8778 T8780 mark that,is
R6956 T8779 T8780 nsubj Runx1,is
R6957 T8780 T8777 ccomp is,indicating
R6958 T8781 T8782 det a,target
R6959 T8782 T8780 attr target,is
R6960 T8783 T8782 prep of,target
R6961 T8784 T8783 pobj repression,of
R6962 T8785 T8782 prep by,target
R6963 T8786 T8785 pobj Runx3,by
R6964 T8787 T8762 punct .,showed
R6965 T8788 T8801 advmod Notably,/
R6966 T8789 T8795 punct ",",expression
R6967 T8790 T8793 nsubj Eo-VP16,up-regulate
R6968 T8791 T8793 aux did,up-regulate
R6969 T8792 T8793 neg not,up-regulate
R6970 T8793 T8795 amod up-regulate,expression
R6971 T8794 T8795 compound perforin,expression
R6972 T8795 T8801 nsubj expression,/
R6973 T8796 T8797 advmod when,expressed
R6974 T8797 T8795 advcl expressed,expression
R6975 T8798 T8797 prep in,expressed
R6976 T8799 T8800 compound Runx3,−
R6977 T8800 T8798 pobj −,in
R6978 T8801 T8801 ROOT /,/
R6979 T8802 T8803 nummod −,cells
R6980 T8803 T8801 dobj cells,/
R6981 T8804 T8801 punct ",",/
R6982 T8805 T8808 advmod even,restored
R6983 T8806 T8808 mark though,restored
R6984 T8807 T8808 nsubj it,restored
R6985 T8808 T8801 advcl restored,/
R6986 T8809 T8810 det the,capacity
R6987 T8810 T8808 dobj capacity,restored
R6988 T8811 T8812 aux to,induce
R6989 T8812 T8810 acl induce,capacity
R6990 T8813 T8814 amod IFN-γ,expression
R6991 T8814 T8812 dobj expression,induce
R6992 T8815 T8812 prep upon,induce
R6993 T8816 T8817 compound TCR,restimulation
R6994 T8817 T8812 advmod restimulation,induce
R6995 T8818 T8819 punct (,Fig.
R6996 T8819 T8817 parataxis Fig.,restimulation
R6997 T8820 T8819 nummod 4,Fig.
R6998 T8821 T8819 punct ",",Fig.
R6999 T8822 T8819 appos A,Fig.
R7000 T8823 T8822 cc and,A
R7001 T8824 T8822 conj B,A
R7002 T8825 T8824 punct ),B
R7003 T8826 T8801 punct .,/
R7004 T8827 T8828 det This,result
R7005 T8828 T8829 nsubj result,suggests
R7006 T8829 T8829 ROOT suggests,suggests
R7007 T8830 T8829 advmod strongly,suggests
R7008 T8831 T8834 mark that,requires
R7009 T8832 T8833 amod perforin,expression
R7010 T8833 T8834 nsubj expression,requires
R7011 T8834 T8829 ccomp requires,suggests
R7012 T8835 T8834 dobj Runx3,requires
R7088 T8911 T8912 compound Runx3,−
R7089 T8912 T8910 pobj −,of
R7090 T8913 T8913 ROOT /,/
R7091 T8914 T8915 nummod −,mice
R7092 T8915 T8913 dobj mice,/
R7093 T8916 T8915 acl immunized,mice
R7094 T8917 T8916 prep with,immunized
R7095 T8918 T8920 amod certain,cells
R7096 T8919 T8920 compound tumor,cells
R7097 T8920 T8917 pobj cells,with
R7098 T8921 T8922 advmod effectively,killed
R7099 T8922 T8913 conj killed,/
R7100 T8923 T8924 det these,targets
R7101 T8924 T8922 dobj targets,killed
R7102 T8925 T8926 punct (,13
R7103 T8926 T8922 npadvmod 13,killed
R7104 T8927 T8926 punct ),13
R7105 T8928 T8913 punct .,/
R7106 T8929 T8943 advmod Therefore,/
R7107 T8930 T8943 punct ",",/
R7108 T8931 T8938 mark although,is
R7109 T8932 T8938 nsubj activation,is
R7110 T8933 T8932 prep of,activation
R7111 T8934 T8937 det the,machinery
R7112 T8935 T8937 compound perforin/granzyme,machinery
R7113 T8936 T8937 compound B,machinery
R7114 T8937 T8933 pobj machinery,of
R7115 T8938 T8943 advcl is,/
R7116 T8939 T8938 acomp defective,is
R7117 T8940 T8939 prep in,defective
R7118 T8941 T8942 compound Runx3,−
R7119 T8942 T8940 pobj −,in
R7041 T8864 T8867 det a,reaction
R7042 T8865 T8867 amod mixed,reaction
R7043 T8866 T8867 compound lymphocyte,reaction
R7044 T8867 T8863 pobj reaction,in
R7045 T8868 T8867 punct (,reaction
R7046 T8869 T8867 appos 12,reaction
R7047 T8870 T8867 punct ),reaction
R7048 T8871 T8859 punct .,showed
R7049 T8872 T8877 advmod However,/
R7050 T8873 T8877 punct ",",/
R7051 T8874 T8876 nmod TCR-stimulated,−
R7052 T8875 T8876 amod Runx3,−
R7053 T8876 T8877 nsubj −,/
R7054 T8877 T8877 ROOT /,/
R7055 T8878 T8881 nummod −,cells
R7056 T8879 T8881 nummod CD8,cells
R7057 T8880 T8881 compound +,cells
R7058 T8881 T8882 nsubj cells,were
R7059 T8882 T8877 ccomp were,/
R7060 T8883 T8884 advmod as,effective
R7061 T8884 T8882 acomp effective,were
R7062 T8885 T8884 prep as,effective
R7063 T8886 T8887 compound WT,cells
R7064 T8887 T8885 pobj cells,as
R7065 T8888 T8887 prep in,cells
R7066 T8889 T8888 pcomp killing,in
R7067 T8890 T8891 compound tumor,cells
R7068 T8891 T8889 dobj cells,killing
R7069 T8892 T8889 prep in,killing
R7070 T8893 T8896 det a,assay
R7071 T8894 T8896 amod redirected,assay
R7072 T8895 T8896 compound CTL,assay
R7073 T8896 T8892 pobj assay,in
R7074 T8897 T8896 punct (,assay
R7075 T8898 T8896 appos 12,assay
R7076 T8899 T8896 punct ),assay
R7077 T8900 T8877 punct .,/
R7078 T8901 T8913 advmod Furthermore,/
R7079 T8902 T8913 punct ",",/
R7080 T8903 T8905 compound CD8,cells
R7081 T8904 T8905 compound +,cells
R7082 T8905 T8913 nsubj cells,/
R7083 T8906 T8905 prep from,cells
R7084 T8907 T8909 det the,cavity
R7085 T8908 T8909 amod peritoneal,cavity
R7086 T8909 T8906 pobj cavity,from
R7087 T8910 T8909 prep of,cavity
R9397 T12003 T12005 amod latter,genes
R9398 T12004 T12005 nummod three,genes
R9399 T12005 T12001 pobj genes,of
R9400 T12006 T11994 punct ",",binds
R9401 T12007 T11994 acl suggesting,binds
R9402 T12008 T12010 det a,effect
R9403 T12009 T12010 amod direct,effect
R9404 T12010 T12007 dobj effect,suggesting
R9405 T12011 T12010 prep on,effect
R9406 T12012 T12013 compound gene,expression
R9407 T12013 T12011 pobj expression,on
R9408 T12014 T11994 punct .,binds
R9409 T12015 T12016 amod Additional,experiments
R9410 T12016 T12018 nsubjpass experiments,needed
R9411 T12017 T12018 auxpass are,needed
R9412 T12018 T12018 ROOT needed,needed
R9413 T12019 T12020 aux to,determine
R9414 T12020 T12018 advcl determine,needed
R9415 T12021 T12025 mark whether,are
R9416 T12022 T12025 nsubj Eomes,are
R9417 T12023 T12022 cc and,Eomes
R9418 T12024 T12022 conj Runx1,Eomes
R9419 T12025 T12020 ccomp are,determine
R9420 T12026 T12025 advmod also,are
R9421 T12027 T12029 amod direct,genes
R9422 T12028 T12029 compound target,genes
R9423 T12029 T12025 attr genes,are
R9424 T12030 T12029 prep of,genes
R7120 T8943 T8951 ccomp /,are
R7121 T8944 T8947 nummod −,cells
R7122 T8945 T8947 nummod CD8,cells
R7123 T8946 T8947 compound +,cells
R7124 T8947 T8943 dobj cells,/
R7125 T8948 T8951 punct ",",are
R7126 T8949 T8950 det these,cells
R7127 T8950 T8951 nsubj cells,are
R7128 T8951 T8951 ROOT are,are
R7129 T8952 T8951 neg not,are
R7130 T8953 T8954 advmod entirely,devoid
R7131 T8954 T8951 acomp devoid,are
R7132 T8955 T8954 prep of,devoid
R7133 T8956 T8957 amod cytolytic,activity
R7134 T8957 T8955 pobj activity,of
R7135 T8958 T8951 cc and,are
R7136 T8959 T8962 aux could,kill
R7137 T8960 T8962 advmod still,kill
R7138 T8961 T8962 advmod effectively,kill
R7139 T8962 T8951 conj kill,are
R7140 T8963 T8962 dobj targets,kill
R7141 T8964 T8962 punct ",",kill
R7142 T8965 T8966 advmod possibly,by
R7143 T8966 T8962 prep by,kill
R7144 T8967 T8968 amod alternative,mechanisms
R7145 T8968 T8966 pobj mechanisms,by
R7146 T8969 T8970 amod such,as
R7147 T8970 T8968 prep as,mechanisms
R7148 T8971 T8972 det the,Fas
R7149 T8972 T8970 pobj Fas,as
R7150 T8973 T8974 compound Fas,ligand
R7151 T8974 T8975 compound ligand,pathway
R7152 T8975 T8962 dobj pathway,kill
R7153 T8976 T8962 punct .,kill
R9306 T11912 T11915 amod Runx3,factors
R9307 T11913 T11912 cc and,Runx3
R9308 T11914 T11912 conj T-box,Runx3
R9309 T11915 T11916 nsubj factors,control
R9310 T11916 T11916 ROOT control,control
R9311 T11917 T11919 det a,program
R9312 T11918 T11919 amod complex,program
R9313 T11919 T11916 dobj program,control
R9314 T11920 T11919 prep of,program
R9315 T11921 T11922 amod transcriptional,regulation
R9316 T11922 T11920 pobj regulation,of
R9317 T11923 T11916 prep during,control
R9318 T11924 T11925 compound CTL,differentiation
R9319 T11925 T11923 pobj differentiation,during
R9320 T11926 T11930 advmod Collectively,provide
R9321 T11927 T11930 punct ",",provide
R9322 T11928 T11929 det these,data
R9323 T11929 T11930 nsubj data,provide
R9324 T11930 T11930 ROOT provide,provide
R9325 T11931 T11930 dobj evidence,provide
R9326 T11932 T11940 mark that,orchestrates
R9327 T11933 T11940 nsubj Runx3,orchestrates
R9328 T11934 T11933 punct ",",Runx3
R9329 T11935 T11936 advmod together,with
R9330 T11936 T11933 prep with,Runx3
R9331 T11937 T11938 amod T-box,factors
R9332 T11938 T11936 pobj factors,with
R9333 T11939 T11940 punct ",",orchestrates
R9334 T11940 T11931 acl orchestrates,evidence
R9335 T11941 T11943 det a,program
R9336 T11942 T11943 amod complex,program
R9337 T11943 T11940 dobj program,orchestrates
R9338 T11944 T11943 prep of,program
R9339 T11945 T11946 amod transcriptional,regulation
R9340 T11946 T11944 pobj regulation,of
R9341 T11947 T11946 prep in,regulation
R9342 T11948 T11947 pcomp differentiating,in
R9343 T11949 T11948 dobj CTL,differentiating
R9344 T11950 T11953 punct (,C
R9345 T11951 T11953 punct Fig.,C
R9346 T11952 T11951 nummod 4,Fig.
R9347 T11953 T11949 appos C,CTL
R9348 T11954 T11953 punct ),C
R9349 T11955 T11930 punct .,provide
R9350 T11956 T11957 nsubj Runx3,is
R9351 T11957 T11957 ROOT is,is
R9352 T11958 T11957 acomp present,is
R9353 T11959 T11958 prep in,present
R9354 T11960 T11964 amod naive,cells
R9355 T11961 T11964 nmod CD8,cells
R9356 T11962 T11964 nummod +,cells
R9357 T11963 T11964 compound T,cells
R9358 T11964 T11959 pobj cells,in
R9359 T11965 T11957 prep before,is
R9360 T11966 T11965 pobj activation,before
R9361 T11967 T11968 punct (,12
R9362 T11968 T11966 appos 12,activation
R9363 T11969 T11957 punct ),is
R9364 T11970 T11957 punct .,is
R9365 T11971 T11972 nsubj It,represses
R9366 T11972 T11972 ROOT represses,represses
R9367 T11973 T11972 dobj Runx1,represses
R9368 T11974 T11972 cc and,represses
R9369 T11975 T11972 conj has,represses
R9370 T11976 T11978 det a,role
R9371 T11977 T11978 amod positive,role
R9372 T11978 T11975 dobj role,has
R9373 T11979 T11978 prep in,role
R9374 T11980 T11981 det the,induction
R9375 T11981 T11979 pobj induction,in
R9376 T11982 T11981 prep of,induction
R9377 T11983 T11982 pobj Eomes,of
R9378 T11984 T11978 punct ",",role
R9379 T11985 T11986 compound granzyme,B
R9380 T11986 T11978 appos B,role
R9381 T11987 T11986 punct ",",B
R9382 T11988 T11986 conj perforin,B
R9383 T11989 T11988 punct ",",perforin
R9384 T11990 T11988 cc and,perforin
R9385 T11991 T11988 conj IFN-γ,perforin
R9386 T11992 T11972 punct .,represses
R9387 T11993 T11994 amod Runx3,binds
R9388 T11994 T11994 ROOT binds,binds
R9389 T11995 T11994 prep to,binds
R9390 T11996 T11995 pobj promoters,to
R9391 T11997 T11996 cc and,promoters
R9392 T11998 T12000 amod putative,regions
R9393 T11999 T12000 amod regulatory,regions
R9394 T12000 T11996 conj regions,promoters
R9395 T12001 T12000 prep of,regions
R9396 T12002 T12005 det the,genes
R9472 T12078 T12077 pcomp of,because
R9473 T12079 T12077 pobj compensation,because
R9474 T12080 T12079 prep by,compensation
R9475 T12081 T12080 pobj Runx1,by
R9476 T12082 T12081 punct ",",Runx1
R9477 T12083 T12084 nsubj which,is
R9478 T12084 T12081 relcl is,Runx1
R9479 T12085 T12084 attr derepressed,is
R9480 T12086 T12085 prep in,derepressed
R9481 T12087 T12088 compound Runx3,−
R9482 T12088 T12086 pobj −,in
R9483 T12089 T12089 ROOT /,/
R9484 T12090 T12091 nummod −,cells
R9485 T12091 T12089 dobj cells,/
R9486 T12092 T12093 punct (,Fig.
R9487 T12093 T12095 nmod Fig.,A
R9488 T12094 T12095 nummod 4,A
R9489 T12095 T12091 appos A,cells
R9490 T12096 T12091 punct ),cells
R9491 T12097 T12089 punct .,/
R9492 T12098 T12099 nsubj Runx3,continues
R9493 T12099 T12099 ROOT continues,continues
R9494 T12100 T12102 aux to,required
R9495 T12101 T12102 auxpass be,required
R9496 T12102 T12099 xcomp required,continues
R9497 T12103 T12102 prep for,required
R9498 T12104 T12105 amod IFN-γ,expression
R9499 T12105 T12103 pobj expression,for
R9500 T12106 T12107 advmod even,at
R9501 T12107 T12105 prep at,expression
R9502 T12108 T12107 pobj day,at
R9503 T12109 T12108 nummod 6,day
R9504 T12110 T12099 punct ",",continues
R9425 T12031 T12030 pobj Runx3,of
R9426 T12032 T12018 punct .,needed
R9427 T12033 T12036 advmod Surprisingly,contributed
R9428 T12034 T12036 punct ",",contributed
R9429 T12035 T12036 nsubj Runx3,contributed
R9430 T12036 T12036 ROOT contributed,contributed
R9431 T12037 T12036 prep to,contributed
R9432 T12038 T12040 det the,expression
R9433 T12039 T12040 amod optimal,expression
R9434 T12040 T12037 pobj expression,to
R9435 T12041 T12040 prep of,expression
R9436 T12042 T12041 pobj TNF,of
R9437 T12043 T12042 punct ",",TNF
R9438 T12044 T12042 conj IL-2,TNF
R9439 T12045 T12044 punct ",",IL-2
R9440 T12046 T12044 cc and,IL-2
R9441 T12047 T12044 conj IFN-γ,IL-2
R9442 T12048 T12040 prep at,expression
R9443 T12049 T12052 nmod day,Fig
R9444 T12050 T12049 nummod 4,day
R9445 T12051 T12052 punct (,Fig
R9446 T12052 T12048 pobj Fig,at
R9447 T12053 T12036 punct .,contributed
R9448 T12054 T12054 ROOT S2,S2
R9449 T12055 T12054 punct ),S2
R9450 T12056 T12054 punct .,S2
R9451 T12057 T12089 prep For,/
R9452 T12058 T12057 pobj TNF,For
R9453 T12059 T12058 cc and,TNF
R9454 T12060 T12058 conj IL-2,TNF
R9455 T12061 T12060 punct ",",IL-2
R9456 T12062 T12063 det the,requirement
R9457 T12063 T12060 appos requirement,IL-2
R9458 T12064 T12063 prep for,requirement
R9459 T12065 T12066 amod Runx3,subsides
R9460 T12066 T12064 pobj subsides,for
R9461 T12067 T12066 prep by,subsides
R9462 T12068 T12067 pobj day,by
R9463 T12069 T12068 nummod 6,day
R9464 T12070 T12071 punct (,Fig.
R9465 T12071 T12073 nmod Fig.,C
R9466 T12072 T12071 nummod 3,Fig.
R9467 T12073 T12060 conj C,IL-2
R9468 T12074 T12073 punct ),C
R9469 T12075 T12057 punct ",",For
R9470 T12076 T12077 advmod possibly,because
R9471 T12077 T12089 prep because,/
R9622 T12228 T12227 pobj IFN-γ,of
R9623 T12229 T12226 prep in,expression
R9624 T12230 T12231 amod restimulated,CTLs
R9625 T12231 T12229 pobj CTLs,in
R9626 T12232 T12233 punct (,8
R9627 T12233 T12226 appos 8,expression
R9628 T12234 T12226 punct ),expression
R9629 T12235 T12219 punct .,substitute
R9630 T12236 T12242 advmod Indeed,contribute
R9631 T12237 T12242 punct ",",contribute
R9632 T12238 T12242 nsubj T-bet,contribute
R9633 T12239 T12238 cc and,T-bet
R9634 T12240 T12238 conj Eomes,T-bet
R9635 T12241 T12242 dep both,contribute
R9636 T12242 T12242 ROOT contribute,contribute
R9637 T12243 T12244 aux to,perforin
R9638 T12244 T12242 xcomp perforin,contribute
R9639 T12245 T12244 dobj expression,perforin
R9640 T12246 T12244 prep in,perforin
R9641 T12247 T12248 compound NK,cells
R9642 T12248 T12246 pobj cells,in
R9643 T12249 T12252 punct (,26
R9644 T12250 T12252 nummod 8,26
R9645 T12251 T12252 punct ",",26
R9646 T12252 T12248 appos 26,cells
R9647 T12253 T12248 punct ),cells
R9648 T12254 T12244 punct ",",perforin
R9649 T12255 T12242 cc and,contribute
R9650 T12256 T12257 nsubj Eomes,induces
R9651 T12257 T12282 ccomp induces,vary
R9652 T12258 T12259 compound granzyme,B
R9653 T12259 T12257 dobj B,induces
R9505 T12111 T12112 advmod perhaps,because
R9506 T12112 T12099 prep because,continues
R9507 T12113 T12112 pcomp of,because
R9508 T12114 T12115 poss its,role
R9509 T12115 T12112 pobj role,because
R9510 T12116 T12115 prep in,role
R9511 T12117 T12118 det the,induction
R9512 T12118 T12116 pobj induction,in
R9513 T12119 T12118 prep of,induction
R9514 T12120 T12121 compound Eomes,expression
R9515 T12121 T12119 pobj expression,of
R9516 T12122 T12123 punct (,Fig.
R9517 T12123 T12115 appos Fig.,role
R9518 T12124 T12125 nummod 4,A
R9519 T12125 T12123 appos A,Fig.
R9520 T12126 T12123 punct ),Fig.
R9521 T12127 T12099 punct .,continues
R9522 T12128 T12130 det An,finding
R9523 T12129 T12130 amod unexpected,finding
R9524 T12130 T12131 nsubj finding,was
R9525 T12131 T12131 ROOT was,was
R9526 T12132 T12143 mark that,are
R9527 T12133 T12137 det the,factors
R9528 T12134 T12137 nummod two,factors
R9529 T12135 T12137 amod T-box,factors
R9530 T12136 T12137 compound transcription,factors
R9531 T12137 T12143 nsubj factors,are
R9532 T12138 T12137 punct ",",factors
R9533 T12139 T12137 appos T-bet,factors
R9534 T12140 T12139 cc and,T-bet
R9535 T12141 T12139 conj Eomes,T-bet
R9536 T12142 T12143 punct ",",are
R9537 T12143 T12131 ccomp are,was
R9538 T12144 T12143 acomp up-regulated,are
R9539 T12145 T12144 prep with,up-regulated
R9540 T12146 T12147 advmod very,different
R9541 T12147 T12148 amod different,kinetics
R9542 T12148 T12145 pobj kinetics,with
R9543 T12149 T12148 prep in,kinetics
R9544 T12150 T12153 nummod CD8,cells
R9545 T12151 T12153 nummod +,cells
R9546 T12152 T12153 compound T,cells
R9547 T12153 T12149 pobj cells,in
R9548 T12154 T12153 prep under,cells
R9549 T12155 T12157 poss our,conditions
R9550 T12156 T12157 compound culture,conditions
R9551 T12157 T12154 pobj conditions,under
R9552 T12158 T12143 cc and,are
R9553 T12159 T12143 conj have,are
R9554 T12160 T12161 amod nonredundant,roles
R9555 T12161 T12159 dobj roles,have
R9556 T12162 T12159 prep in,have
R9557 T12163 T12165 det the,expression
R9558 T12164 T12165 amod subsequent,expression
R9559 T12165 T12162 pobj expression,in
R9560 T12166 T12165 prep of,expression
R9561 T12167 T12169 amod key,proteins
R9562 T12168 T12169 compound effector,proteins
R9563 T12169 T12166 pobj proteins,of
R9564 T12170 T12173 punct (,C
R9565 T12171 T12173 nmod Fig.,C
R9566 T12172 T12171 nummod 4,Fig.
R9567 T12173 T12165 appos C,expression
R9568 T12174 T12173 punct ),C
R9569 T12175 T12131 punct .,was
R9570 T12176 T12178 nsubjpass T-bet,needed
R9571 T12177 T12178 auxpass is,needed
R9572 T12178 T12178 ROOT needed,needed
R9573 T12179 T12178 advmod early,needed
R9574 T12180 T12181 aux to,confer
R9575 T12181 T12178 advcl confer,needed
R9576 T12182 T12181 prep on,confer
R9577 T12183 T12187 amod activated,cells
R9578 T12184 T12186 compound CD8,T
R9579 T12185 T12186 compound +,T
R9580 T12186 T12187 compound T,cells
R9581 T12187 T12182 pobj cells,on
R9582 T12188 T12189 det the,competence
R9583 T12189 T12181 dobj competence,confer
R9584 T12190 T12191 aux to,produce
R9585 T12191 T12189 relcl produce,competence
R9586 T12192 T12191 dobj IFN-γ,produce
R9587 T12193 T12191 prep upon,produce
R9588 T12194 T12193 pobj restimulation,upon
R9589 T12195 T12178 punct ",",needed
R9590 T12196 T12178 cc but,needed
R9591 T12197 T12198 poss its,function
R9592 T12198 T12199 nsubj function,is
R9593 T12199 T12178 conj is,needed
R9594 T12200 T12201 advmod less,important
R9595 T12201 T12199 acomp important,is
R9596 T12202 T12201 prep at,important
R9597 T12203 T12204 amod later,times
R9598 T12204 T12202 pobj times,at
R9599 T12205 T12199 punct .,is
R9600 T12206 T12219 nsubj Eomes,substitute
R9601 T12207 T12206 punct ",",Eomes
R9602 T12208 T12210 nsubjpass which,induced
R9603 T12209 T12210 auxpass is,induced
R9604 T12210 T12206 relcl induced,Eomes
R9605 T12211 T12210 advmod late,induced
R9606 T12212 T12210 cc and,induced
R9607 T12213 T12210 conj functions,induced
R9608 T12214 T12213 amod downstream,functions
R9609 T12215 T12214 prep of,downstream
R9610 T12216 T12215 pobj Runx3,of
R9611 T12217 T12219 punct ",",substitute
R9612 T12218 T12219 aux may,substitute
R9613 T12219 T12219 ROOT substitute,substitute
R9614 T12220 T12219 prep for,substitute
R9615 T12221 T12220 pobj T-bet,for
R9616 T12222 T12219 prep in,substitute
R9617 T12223 T12222 pcomp promoting,in
R9618 T12224 T12226 det the,expression
R9619 T12225 T12226 amod acute,expression
R9620 T12226 T12223 dobj expression,promoting
R9621 T12227 T12226 prep of,expression
R9697 T12303 T12304 amod other,markers
R9698 T12304 T12301 dobj markers,induce
R9699 T12305 T12304 prep of,markers
R9700 T12306 T12307 compound CTL,function
R9701 T12307 T12305 pobj function,of
R9702 T12308 T12307 punct ",",function
R9703 T12309 T12307 conj Prf1,function
R9704 T12310 T12309 cc and,Prf1
R9705 T12311 T12309 conj Gzmb,Prf1
R9706 T12312 T12313 punct (,Fig.
R9707 T12313 T12315 nmod Fig.,C
R9708 T12314 T12315 nummod 4,C
R9709 T12315 T12309 conj C,Prf1
R9710 T12316 T12315 punct ),C
R9711 T12317 T12295 punct .,appeared
R9712 T12318 T12324 advmod Rather,involved
R9713 T12319 T12324 punct ",",involved
R9714 T12320 T12324 nsubjpass T-bet,involved
R9715 T12321 T12320 cc and,T-bet
R9716 T12322 T12320 conj Eomes,T-bet
R9717 T12323 T12324 auxpass were,involved
R9718 T12324 T12344 ccomp involved,preceded
R9719 T12325 T12324 prep in,involved
R9720 T12326 T12325 pcomp regulating,in
R9721 T12327 T12328 compound granzyme,B
R9722 T12328 T12326 dobj B,regulating
R9723 T12329 T12328 cc and,B
R9724 T12330 T12331 compound perforin,expression
R9725 T12331 T12328 conj expression,B
R9726 T12332 T12326 punct ",",regulating
R9727 T12333 T12324 advmod respectively,involved
R9728 T12334 T12324 punct :,involved
R9729 T12335 T12344 nsubj up-regulation,preceded
R9730 T12336 T12335 prep of,up-regulation
R9731 T12337 T12336 pobj T-bet,of
R9732 T12338 T12337 cc and,T-bet
R9733 T12339 T12340 compound Eomes,mRNA
R9734 T12340 T12337 conj mRNA,T-bet
R9735 T12341 T12340 cc and,mRNA
R9736 T12342 T12340 conj protein,mRNA
R9737 T12343 T12344 advmod closely,preceded
R9654 T12260 T12261 advmod as,effectively
R9655 T12261 T12257 advmod effectively,induces
R9656 T12262 T12257 prep as,induces
R9657 T12263 T12262 pobj T-bet,as
R9658 T12264 T12263 prep in,T-bet
R9659 T12265 T12264 pcomp developing,in
R9660 T12266 T12267 amod Th2,cells
R9661 T12267 T12265 dobj cells,developing
R9662 T12268 T12267 punct (,cells
R9663 T12269 T12267 appos 7,cells
R9664 T12270 T12267 punct ),cells
R9665 T12271 T12257 punct ;,induces
R9666 T12272 T12282 advmod thus,vary
R9667 T12273 T12282 punct ",",vary
R9668 T12274 T12276 det the,roles
R9669 T12275 T12276 amod relative,roles
R9670 T12276 T12282 nsubj roles,vary
R9671 T12277 T12276 prep of,roles
R9672 T12278 T12281 det these,factors
R9673 T12279 T12281 amod T-box,factors
R9674 T12280 T12281 compound transcription,factors
R9675 T12281 T12277 pobj factors,of
R9676 T12282 T12242 conj vary,contribute
R9677 T12283 T12282 prep depending,vary
R9678 T12284 T12283 prep on,depending
R9679 T12285 T12286 compound cell,type
R9680 T12286 T12284 pobj type,on
R9681 T12287 T12282 punct .,vary
R9682 T12288 T12295 advmod Surprisingly,appeared
R9683 T12289 T12295 punct ",",appeared
R9684 T12290 T12295 advmod however,appeared
R9685 T12291 T12295 punct ",",appeared
R9686 T12292 T12295 nsubj Eomes,appeared
R9687 T12293 T12292 cc and,Eomes
R9688 T12294 T12292 conj T-bet,Eomes
R9689 T12295 T12295 ROOT appeared,appeared
R9690 T12296 T12295 oprd nonredundant,appeared
R9691 T12297 T12295 prep in,appeared
R9692 T12298 T12299 poss their,ability
R9693 T12299 T12297 pobj ability,in
R9694 T12300 T12301 aux to,induce
R9695 T12301 T12299 acl induce,ability
R9696 T12302 T12304 nummod two,markers
R9847 T12453 T12454 nsubj data,are
R9848 T12454 T12454 ROOT are,are
R9849 T12455 T12454 acomp consistent,are
R9850 T12456 T12455 prep with,consistent
R9851 T12457 T12459 det a,network
R9852 T12458 T12459 amod transcriptional,network
R9853 T12459 T12462 nsubj network,preexisting
R9854 T12460 T12462 prep in,preexisting
R9855 T12461 T12460 pobj which,in
R9856 T12462 T12456 pobj preexisting,with
R9857 T12463 T12464 amod Runx3,cooperates
R9858 T12464 T12462 dobj cooperates,preexisting
R9859 T12465 T12462 prep with,preexisting
R9860 T12466 T12469 det the,factors
R9861 T12467 T12469 amod induced,factors
R9862 T12468 T12469 compound T-box,factors
R9863 T12469 T12465 pobj factors,with
R9864 T12470 T12475 nmod T-bet,signals
R9865 T12471 T12470 cc and,T-bet
R9866 T12472 T12470 conj Eomes,T-bet
R9867 T12473 T12472 cc and,Eomes
R9868 T12474 T12472 conj IL-2Rβ,Eomes
R9869 T12475 T12469 appos signals,factors
R9870 T12476 T12478 punct (,data
R9871 T12477 T12478 amod unpublished,data
R9872 T12478 T12475 appos data,signals
R9873 T12479 T12475 punct ),signals
R9874 T12480 T12481 aux to,orchestrate
R9875 T12481 T12475 advcl orchestrate,signals
R9876 T12482 T12483 compound CTL,differentiation
R9877 T12483 T12481 dobj differentiation,orchestrate
R9878 T12484 T12487 punct (,C
R9879 T12485 T12487 nsubj Fig.,C
R9880 T12486 T12485 nummod 4,Fig.
R9881 T12487 T12475 appos C,signals
R9882 T12488 T12454 punct ),are
R9883 T12489 T12454 punct .,are
R9884 T12490 T12491 poss Our,data
R9738 T12344 T12344 ROOT preceded,preceded
R9739 T12345 T12344 dobj up-regulation,preceded
R9740 T12346 T12345 prep of,up-regulation
R9741 T12347 T12346 pobj Gzmb,of
R9742 T12348 T12347 cc and,Gzmb
R9743 T12349 T12350 compound Prf1,mRNA
R9744 T12350 T12347 conj mRNA,Gzmb
R9745 T12351 T12350 cc and,mRNA
R9746 T12352 T12350 conj protein,mRNA
R9747 T12353 T12344 punct ",",preceded
R9748 T12354 T12344 advmod respectively,preceded
R9749 T12355 T12344 punct .,preceded
R9750 T12356 T12357 nsubj T-bet,had
R9751 T12357 T12357 ROOT had,had
R9752 T12358 T12359 det no,role
R9753 T12359 T12357 dobj role,had
R9754 T12360 T12359 prep in,role
R9755 T12361 T12362 amod perforin,expression
R9756 T12362 T12360 pobj expression,in
R9757 T12363 T12362 prep under,expression
R9758 T12364 T12366 poss our,conditions
R9759 T12365 T12366 compound culture,conditions
R9760 T12366 T12363 pobj conditions,under
R9761 T12367 T12357 punct ",",had
R9762 T12368 T12357 cc and,had
R9763 T12369 T12372 nsubj Eo-VP16,affect
R9764 T12370 T12372 aux did,affect
R9765 T12371 T12372 neg not,affect
R9766 T12372 T12357 conj affect,had
R9767 T12373 T12375 amod granzyme,expression
R9768 T12374 T12375 compound B,expression
R9769 T12375 T12372 dobj expression,affect
R9770 T12376 T12377 advmod when,expressed
R9771 T12377 T12372 advcl expressed,affect
R9772 T12378 T12377 prep in,expressed
R9773 T12379 T12381 quantmod T-bet,/
R9774 T12380 T12381 nummod −,/
R9775 T12381 T12388 nummod /,cells
R9776 T12382 T12388 nmod −,cells
R9777 T12383 T12382 cc or,−
R9778 T12384 T12382 conj Runx3,−
R9779 T12385 T12388 compound −,cells
R9780 T12386 T12388 compound /,cells
R9781 T12387 T12388 compound −,cells
R9782 T12388 T12378 pobj cells,in
R9783 T12389 T12372 punct .,affect
R9784 T12390 T12394 mark Because,die
R9785 T12391 T12393 amod conventional,mice
R9786 T12392 T12393 amod Eomes-deficient,mice
R9787 T12393 T12394 nsubj mice,die
R9788 T12394 T12426 advcl die,were
R9789 T12395 T12400 mark before,isolated
R9790 T12396 T12397 compound precursor,cells
R9791 T12397 T12400 nsubjpass cells,isolated
R9792 T12398 T12400 aux can,isolated
R9793 T12399 T12400 auxpass be,isolated
R9794 T12400 T12394 advcl isolated,die
R9795 T12401 T12400 prep for,isolated
R9796 T12402 T12403 compound bone,marrow
R9797 T12403 T12404 compound marrow,transfers
R9798 T12404 T12401 pobj transfers,for
R9799 T12405 T12404 punct (,transfers
R9800 T12406 T12404 appos 27,transfers
R9801 T12407 T12404 punct ),transfers
R9802 T12408 T12400 cc and,isolated
R9803 T12409 T12413 mark because,deficient
R9804 T12410 T12411 compound T,cells
R9805 T12411 T12413 nsubj cells,deficient
R9806 T12412 T12413 advmod conditionally,deficient
R9807 T12413 T12394 advcl deficient,die
R9808 T12414 T12413 prep in,deficient
R9809 T12415 T12414 pobj Eomes,in
R9810 T12416 T12420 aux have,described
R9811 T12417 T12418 advmod only,recently
R9812 T12418 T12420 advmod recently,described
R9813 T12419 T12420 auxpass been,described
R9814 T12420 T12394 conj described,die
R9815 T12421 T12420 punct (,described
R9816 T12422 T12420 npadvmod 9,described
R9817 T12423 T12420 punct ),described
R9818 T12424 T12426 punct ",",were
R9819 T12425 T12426 nsubj we,were
R9820 T12426 T12426 ROOT were,were
R9821 T12427 T12426 acomp unable,were
R9822 T12428 T12429 aux to,introduce
R9823 T12429 T12427 xcomp introduce,unable
R9824 T12430 T12429 dobj Runx3,introduce
R9825 T12431 T12429 prep into,introduce
R9826 T12432 T12435 compound Eomes-deficient,T
R9827 T12433 T12435 compound CD8,T
R9828 T12434 T12435 compound +,T
R9829 T12435 T12436 compound T,cells
R9830 T12436 T12431 pobj cells,into
R9831 T12437 T12438 aux to,test
R9832 T12438 T12429 advcl test,introduce
R9833 T12439 T12442 advmod formally,cooperated
R9834 T12440 T12442 mark whether,cooperated
R9835 T12441 T12442 nsubj Eomes,cooperated
R9836 T12442 T12426 advcl cooperated,were
R9837 T12443 T12442 prep with,cooperated
R9838 T12444 T12443 pobj Runx3,with
R9839 T12445 T12446 aux to,induce
R9840 T12446 T12442 advcl induce,cooperated
R9841 T12447 T12448 amod perforin,expression
R9842 T12448 T12446 dobj expression,induce
R9843 T12449 T12426 punct .,were
R9844 T12450 T12454 advmod Collectively,are
R9845 T12451 T12454 punct ",",are
R9846 T12452 T12453 poss our,data
R9922 T12528 T12529 auxpass is,induced
R9923 T12529 T12539 csubj induced,induces
R9924 T12530 T12529 agent by,induced
R9925 T12531 T12532 compound TCR,signals
R9926 T12532 T12530 pobj signals,by
R9927 T12533 T12532 cc and,signals
R9928 T12534 T12532 conj IFN-γ,signals
R9929 T12535 T12534 punct ",",IFN-γ
R9930 T12536 T12530 cc and,by
R9931 T12537 T12539 prep in,induces
R9932 T12538 T12537 pobj turn,in
R9933 T12539 T12539 ROOT induces,induces
R9934 T12540 T12539 dobj Runx3,induces
R9935 T12541 T12540 punct (,Runx3
R9936 T12542 T12540 appos 15,Runx3
R9937 T12543 T12540 punct ),Runx3
R9938 T12544 T12539 punct ",",induces
R9939 T12545 T12556 mark whereas,required
R9940 T12546 T12556 prep in,required
R9941 T12547 T12546 pcomp differentiating,in
R9942 T12548 T12551 nmod CD8,cells
R9943 T12549 T12551 nummod +,cells
R9944 T12550 T12551 compound T,cells
R9945 T12551 T12547 dobj cells,differentiating
R9946 T12552 T12556 punct ",",required
R9947 T12553 T12554 compound preexisting,Runx3
R9948 T12554 T12556 nsubjpass Runx3,required
R9949 T12555 T12556 auxpass is,required
R9950 T12556 T12539 advcl required,induces
R9951 T12557 T12558 aux to,induce
R9952 T12558 T12556 advcl induce,required
R9953 T12559 T12562 det the,factor
R9954 T12560 T12562 compound T-box,factor
R9955 T12561 T12562 compound transcription,factor
R9956 T12562 T12558 dobj factor,induce
R9957 T12563 T12562 appos Eomes,factor
R9958 T12564 T12539 punct .,induces
R9959 T12565 T12566 amod Whole-genome,experiments
R9960 T12566 T12574 nsubjpass experiments,required
R9961 T12567 T12566 prep in,experiments
R9962 T12568 T12571 det these,systems
R9963 T12569 T12571 nummod and,systems
R9964 T12570 T12571 amod other,systems
R9965 T12571 T12567 pobj systems,in
R9966 T12572 T12574 aux will,required
R9967 T12573 T12574 auxpass be,required
R9968 T12574 T12574 ROOT required,required
R9969 T12575 T12576 aux to,establish
R9970 T12576 T12574 advcl establish,required
R9885 T12491 T12492 nsubj data,recall
R9886 T12492 T12492 ROOT recall,recall
R9887 T12493 T12495 det the,interaction
R9888 T12494 T12495 amod feed-forward,interaction
R9889 T12495 T12492 dobj interaction,recall
R9890 T12496 T12495 prep between,interaction
R9891 T12497 T12496 pobj T-bet,between
R9892 T12498 T12497 cc and,T-bet
R9893 T12499 T12497 conj Runx3,T-bet
R9894 T12500 T12503 dobj that,described
R9895 T12501 T12503 nsubj we,described
R9896 T12502 T12503 advmod previously,described
R9897 T12503 T12495 relcl described,interaction
R9898 T12504 T12503 prep in,described
R9899 T12505 T12506 compound CD4,+
R9900 T12506 T12511 nmod +,cells
R9901 T12507 T12506 punct (,+
R9902 T12508 T12506 appos Th1,+
R9903 T12509 T12506 punct ),+
R9904 T12510 T12511 compound T,cells
R9905 T12511 T12504 pobj cells,in
R9906 T12512 T12495 punct (,interaction
R9907 T12513 T12495 appos 15,interaction
R9908 T12514 T12495 punct ),interaction
R9909 T12515 T12492 cc but,recall
R9910 T12516 T12492 conj are,recall
R9911 T12517 T12516 acomp distinct,are
R9912 T12518 T12517 prep in,distinct
R9913 T12519 T12520 nummod two,respects
R9914 T12520 T12518 pobj respects,in
R9915 T12521 T12529 punct :,induced
R9916 T12522 T12529 prep in,induced
R9917 T12523 T12522 pcomp differentiating,in
R9918 T12524 T12525 amod Th1,cells
R9919 T12525 T12523 dobj cells,differentiating
R9920 T12526 T12529 punct ",",induced
R9921 T12527 T12529 nsubjpass T-bet,induced
R10888 T13934 T13934 ROOT School,School
R10889 T13935 T13934 prep of,School
R10890 T13936 T13937 compound Public,Health
R10891 T13937 T13935 pobj Health,of
R10892 T13938 T13934 punct ",",School
R10893 T13939 T13934 conj Boston,School
R10894 T13940 T13939 punct ",",Boston
R10895 T13941 T13939 conj MA,Boston
R10896 T13942 T13939 punct ),Boston
R10897 T13943 T13928 punct .,provided
R10898 T13944 T13946 det The,reagents
R10899 T13945 T13946 amod following,reagents
R10900 T13946 T13948 nsubjpass reagents,used
R10901 T13947 T13948 auxpass were,used
R10902 T13948 T13948 ROOT used,used
R10903 T13949 T13948 prep for,used
R10904 T13950 T13951 det the,experiments
R10905 T13951 T13949 pobj experiments,for
R10906 T13952 T13951 acl presented,experiments
R10907 T13953 T13952 prep in,presented
R10908 T13954 T13955 det this,report
R10909 T13955 T13953 pobj report,in
R10910 T13956 T13951 punct :,experiments
R10911 T13957 T13958 compound Annexin,V
R10912 T13958 T13951 appos V,experiments
R10913 T13959 T13962 compound FITC,Kit
R10914 T13960 T13962 compound Apoptosis,Kit
R10915 T13961 T13962 compound Detection,Kit
R10916 T13962 T13958 conj Kit,V
R10917 T13963 T13962 punct (,Kit
R10918 T13964 T13962 appos BD,Kit
R10919 T13965 T13962 punct ),Kit
R10920 T13966 T13962 punct ",",Kit
R10921 T13967 T13962 conj CD8,Kit
R10922 T13968 T13970 amod Negative,Kit
R10923 T13969 T13970 compound Isolation,Kit
R10924 T13970 T13962 conj Kit,Kit
R10925 T13971 T13970 punct (,Kit
R10926 T13972 T13970 nmod Invitrogen,Kit
R10927 T13973 T13970 punct ),Kit
R10928 T13974 T13970 punct ",",Kit
R10929 T13975 T13976 compound CD8,MicroBeads
R10930 T13976 T13958 conj MicroBeads,V
R10931 T13977 T13976 punct (,MicroBeads
R10932 T13978 T13979 compound Miltenyi,Biotec
R9971 T12577 T12586 mark whether,is
R9972 T12578 T12586 nsubj cooperation,is
R9973 T12579 T12578 prep between,cooperation
R9974 T12580 T12585 nmod T-box,factors
R9975 T12581 T12580 cc and,T-box
R9976 T12582 T12580 conj Runx,T-box
R9977 T12583 T12584 compound family,transcription
R9978 T12584 T12585 compound transcription,factors
R9979 T12585 T12579 pobj factors,between
R9980 T12586 T12576 ccomp is,establish
R9981 T12587 T12589 det a,feature
R9982 T12588 T12589 amod general,feature
R9983 T12589 T12586 attr feature,is
R9984 T12590 T12589 prep of,feature
R9985 T12591 T12593 amod cellular,programs
R9986 T12592 T12593 compound differentiation,programs
R9987 T12593 T12590 pobj programs,of
R9988 T12594 T12574 punct .,required
R10784 T13830 T13830 ROOT Antibodies,Antibodies
R10785 T13831 T13830 cc and,Antibodies
R10786 T13832 T13830 conj reagents,Antibodies
R10787 T13833 T13830 punct .,Antibodies
R10788 T13834 T13836 det The,antibodies
R10789 T13835 T13836 amod following,antibodies
R10790 T13836 T13844 nsubjpass antibodies,obtained
R10791 T13837 T13836 acl used,antibodies
R10792 T13838 T13837 prep for,used
R10793 T13839 T13842 nmod intracellular,stains
R10794 T13840 T13839 cc or,intracellular
R10795 T13841 T13839 conj surface,intracellular
R10796 T13842 T13838 pobj stains,for
R10797 T13843 T13844 auxpass were,obtained
R10798 T13844 T13844 ROOT obtained,obtained
R10799 T13845 T13844 prep from,obtained
R10800 T13846 T13845 pobj eBioscience,from
R10801 T13847 T13844 punct :,obtained
R10802 T13848 T13849 amod anti,IL-2
R10803 T13849 T13849 ROOT IL-2,IL-2
R10804 T13850 T13849 punct ",",IL-2
R10805 T13851 T13849 appos anti,IL-2
R10806 T13852 T13858 nmod IFN-γ,B
R10807 T13853 T13852 punct ",",IFN-γ
R10808 T13854 T13852 conj anti-TNF,IFN-γ
R10809 T13855 T13856 punct ",",anti
R10810 T13856 T13858 amod anti,B
R10811 T13857 T13858 compound granzyme,B
R10812 T13858 T13849 appos B,IL-2
R10813 T13859 T13858 punct ",",B
R10814 T13860 T13858 conj anti-CD8,B
R10815 T13861 T13860 punct ",",anti-CD8
R10816 T13862 T13860 conj anti-CD25,anti-CD8
R10817 T13863 T13862 punct ",",anti-CD25
R10818 T13864 T13862 cc and,anti-CD25
R10819 T13865 T13862 conj anti-CD44,anti-CD25
R10820 T13866 T13858 punct .,B
R10821 T13867 T13869 nsubjpass Anti-CD69,purchased
R10822 T13868 T13869 auxpass was,purchased
R10823 T13869 T13869 ROOT purchased,purchased
R10824 T13870 T13869 prep from,purchased
R10825 T13871 T13870 pobj BD,from
R10826 T13872 T13869 punct .,purchased
R10827 T13873 T13881 prep For,obtained
R10828 T13874 T13875 compound ChIP,experiments
R10829 T13875 T13873 pobj experiments,For
R10830 T13876 T13881 punct ",",obtained
R10831 T13877 T13879 det the,antibody
R10832 T13878 T13879 amod anti-Eomes,antibody
R10833 T13879 T13881 nsubjpass antibody,obtained
R10834 T13880 T13881 auxpass was,obtained
R10835 T13881 T13881 ROOT obtained,obtained
R10836 T13882 T13881 prep from,obtained
R10837 T13883 T13882 pobj Abcam,from
R10838 T13884 T13883 cc and,Abcam
R10839 T13885 T13887 det the,antibody
R10840 T13886 T13887 amod anti-Runx3,antibody
R10841 T13887 T13883 conj antibody,Abcam
R10842 T13888 T13889 auxpass was,produced
R10843 T13889 T13881 conj produced,obtained
R10844 T13890 T13889 agent by,produced
R10845 T13891 T13893 det the,laboratory
R10846 T13892 T13893 compound Groner,laboratory
R10847 T13893 T13890 pobj laboratory,by
R10848 T13894 T13881 punct .,obtained
R10849 T13895 T13897 det The,antibodies
R10850 T13896 T13897 amod following,antibodies
R10851 T13897 T13899 nsubjpass antibodies,used
R10852 T13898 T13899 auxpass were,used
R10853 T13899 T13899 ROOT used,used
R10854 T13900 T13899 prep for,used
R10855 T13901 T13900 pcomp immunoblotting,for
R10856 T13902 T13903 punct :,antiperforin
R10857 T13903 T13899 npadvmod antiperforin,used
R10858 T13904 T13905 punct (,Abcam
R10859 T13905 T13903 appos Abcam,antiperforin
R10860 T13906 T13905 punct ),Abcam
R10861 T13907 T13903 punct ",",antiperforin
R10862 T13908 T13903 appos anti-Eomes,antiperforin
R10863 T13909 T13910 punct (,Abcam
R10864 T13910 T13908 appos Abcam,anti-Eomes
R10865 T13911 T13910 punct ),Abcam
R10866 T13912 T13908 punct ",",anti-Eomes
R10867 T13913 T13908 cc and,anti-Eomes
R10868 T13914 T13908 conj anti,anti-Eomes
R10869 T13915 T13921 compound Pol-II,Inc.
R10870 T13916 T13919 punct (,Biotechnology
R10871 T13917 T13918 compound Santa,Cruz
R10872 T13918 T13919 compound Cruz,Biotechnology
R10873 T13919 T13915 appos Biotechnology,Pol-II
R10874 T13920 T13919 punct ",",Biotechnology
R10875 T13921 T13903 appos Inc.,antiperforin
R10876 T13922 T13903 punct ),antiperforin
R10877 T13923 T13899 punct .,used
R10878 T13924 T13926 det The,antibody
R10879 T13925 T13926 compound T-bet,antibody
R10880 T13926 T13928 nsubjpass antibody,provided
R10881 T13927 T13928 auxpass was,provided
R10882 T13928 T13928 ROOT provided,provided
R10883 T13929 T13928 agent by,provided
R10884 T13930 T13931 compound L.,Glimcher
R10885 T13931 T13929 pobj Glimcher,by
R10886 T13932 T13934 punct (,School
R10887 T13933 T13934 compound Harvard,School
R10963 T14009 T14010 nummod 10,mM
R10964 T14010 T14015 nsubjpass mM,prepared
R10965 T14011 T14010 prep of,mM
R10966 T14012 T14013 compound stock,solutions
R10967 T14013 T14011 pobj solutions,of
R10968 T14014 T14015 auxpass was,prepared
R10969 T14015 T14000 conj prepared,synthesized
R10970 T14016 T14015 prep in,prepared
R10971 T14017 T14016 pobj DMSO,in
R10972 T14018 T14015 punct .,prepared
R11155 T14382 T14382 ROOT Isolation,Isolation
R11156 T14383 T14382 cc and,Isolation
R11157 T14384 T14382 conj culture,Isolation
R11158 T14385 T14382 prep of,Isolation
R11159 T14386 T14390 amod primary,cells
R11160 T14387 T14390 nmod CD8,cells
R11161 T14388 T14390 nummod +,cells
R11162 T14389 T14390 compound T,cells
R11163 T14390 T14385 pobj cells,of
R11164 T14391 T14382 punct .,Isolation
R11165 T14392 T14395 nummod CD8,cells
R11166 T14393 T14395 nummod +,cells
R11167 T14394 T14395 compound T,cells
R11168 T14395 T14426 nsubjpass cells,purified
R11169 T14396 T14395 prep from,cells
R11170 T14397 T14396 pobj 4,from
R11171 T14398 T14399 compound 8-wk-old,Tcra
R11172 T14399 T14426 nsubjpass Tcra,purified
R11173 T14400 T14406 nmod −,transgenic
R11174 T14401 T14406 nmod /,transgenic
R11175 T14402 T14406 nmod −,transgenic
R11176 T14403 T14406 nmod ×,transgenic
R11177 T14404 T14406 nmod P14,transgenic
R11178 T14405 T14406 nmod TCR,transgenic
R11179 T14406 T14412 nmod transgenic,WT
R10933 T13979 T13976 appos Biotec,MicroBeads
R10934 T13980 T13976 punct ),MicroBeads
R10935 T13981 T13958 punct ",",V
R10936 T13982 T13958 cc and,V
R10937 T13983 T13985 compound SYBR,PCR
R10938 T13984 T13985 compound Green,PCR
R10939 T13985 T13987 compound PCR,Reagents
R10940 T13986 T13987 compound Core,Reagents
R10941 T13987 T13958 conj Reagents,V
R10942 T13988 T13990 punct (,Biosystems
R10943 T13989 T13990 compound Applied,Biosystems
R10944 T13990 T13987 parataxis Biosystems,Reagents
R10945 T13991 T13990 punct ),Biosystems
R10946 T13992 T13948 punct .,used
R10947 T13993 T13995 det The,peptide
R10948 T13994 T13995 amod Gp33,peptide
R10949 T13995 T14000 nsubjpass peptide,synthesized
R10950 T13996 T13995 punct (,peptide
R10951 T13997 T13995 appos KAVYNFATC,peptide
R10952 T13998 T13995 punct ),peptide
R10953 T13999 T14000 auxpass was,synthesized
R10954 T14000 T14000 ROOT synthesized,synthesized
R10955 T14001 T14000 agent by,synthesized
R10956 T14002 T14006 det the,Facility
R10957 T14003 T14004 compound Tufts,University
R10958 T14004 T14006 compound University,Facility
R10959 T14005 T14006 compound Core,Facility
R10960 T14006 T14001 pobj Facility,by
R10961 T14007 T14000 punct ",",synthesized
R10962 T14008 T14000 cc and,synthesized
R11265 T14492 T14497 det the,Care
R11266 T14493 T14495 compound Harvard,School
R11267 T14494 T14495 compound Medical,School
R11268 T14495 T14497 compound School,Care
R11269 T14496 T14497 compound Animal,Care
R11270 T14497 T14490 conj Care,Institute
R11271 T14498 T14497 cc and,Care
R11272 T14499 T14497 conj Use,Care
R11273 T14500 T14497 conj Committees,Care
R11274 T14501 T14474 punct .,maintained
R11275 T14502 T14511 prep For,cultured
R11276 T14503 T14502 pobj stimulation,For
R11277 T14504 T14511 punct ",",cultured
R11278 T14505 T14509 amod purified,cells
R11279 T14506 T14509 nmod CD8,cells
R11280 T14507 T14509 nummod +,cells
R11281 T14508 T14509 compound T,cells
R11282 T14509 T14511 nsubjpass cells,cultured
R11283 T14510 T14511 auxpass were,cultured
R11284 T14511 T14511 ROOT cultured,cultured
R11285 T14512 T14511 prep at,cultured
R11286 T14513 T14514 nummod 106,cells/ml
R11287 T14514 T14512 pobj cells/ml,at
R11288 T14515 T14517 punct (,ml
R11289 T14516 T14517 nummod 10,ml
R11290 T14517 T14514 appos ml,cells/ml
R11291 T14518 T14517 punct ),ml
R11292 T14519 T14511 prep in,cultured
R11293 T14520 T14521 nummod T25,flasks
R11294 T14521 T14519 pobj flasks,in
R11295 T14522 T14511 conj coated,cultured
R11296 T14523 T14522 prep with,coated
R11297 T14524 T14525 nummod 1,μg
R11298 T14525 T14527 compound μg,ml
R11299 T14526 T14527 compound /,ml
R11300 T14527 T14523 pobj ml,with
R11301 T14528 T14527 appos each,ml
R11302 T14529 T14528 prep of,each
R11303 T14530 T14529 pobj anti-CD3,of
R11304 T14531 T14533 punct (,2C11
R11305 T14532 T14533 compound clone,2C11
R11306 T14533 T14530 appos 2C11,anti-CD3
R11307 T14534 T14533 punct ),2C11
R11308 T14535 T14530 cc and,anti-CD3
R11309 T14536 T14530 conj anti-CD28,anti-CD3
R11310 T14537 T14538 punct (,clone
R11311 T14538 T14536 appos clone,anti-CD28
R11312 T14539 T14538 nummod 37.51,clone
R11313 T14540 T14538 punct ),clone
R11314 T14541 T14538 prep by,clone
R11315 T14542 T14541 pobj pretreatment,by
R11316 T14543 T14538 prep with,clone
R11317 T14544 T14545 nummod 300,μg
R11318 T14545 T14549 nmod μg,anti
R11319 T14546 T14549 nmod /,anti
R11180 T14407 T14408 punct (,Taconic
R11181 T14408 T14406 appos Taconic,transgenic
R11182 T14409 T14408 punct ),Taconic
R11183 T14410 T14412 punct ",",WT
R11184 T14411 T14412 compound C57BL/6J,WT
R11185 T14412 T14399 conj WT,Tcra
R11186 T14413 T14412 punct ",",WT
R11187 T14414 T14412 cc or,WT
R11188 T14415 T14418 nummod Tbx21,−
R11189 T14416 T14418 nummod −,−
R11190 T14417 T14418 compound /,−
R11191 T14418 T14412 conj −,WT
R11192 T14419 T14422 punct (,Laboratory
R11193 T14420 T14422 det The,Laboratory
R11194 T14421 T14422 compound Jackson,Laboratory
R11195 T14422 T14418 appos Laboratory,−
R11196 T14423 T14422 punct ),Laboratory
R11197 T14424 T14418 conj mice,−
R11198 T14425 T14426 auxpass were,purified
R11199 T14426 T14426 ROOT purified,purified
R11200 T14427 T14426 punct (,purified
R11201 T14428 T14431 compound >,purity
R11202 T14429 T14430 nummod 95,%
R11203 T14430 T14431 compound %,purity
R11204 T14431 T14426 appos purity,purified
R11205 T14432 T14426 punct ),purified
R11206 T14433 T14426 agent by,purified
R11207 T14434 T14435 amod negative,selection
R11208 T14435 T14433 pobj selection,by
R11209 T14436 T14437 punct (,Invitrogen
R11210 T14437 T14435 appos Invitrogen,selection
R11211 T14438 T14437 punct ),Invitrogen
R11212 T14439 T14435 prep from,selection
R11213 T14440 T14441 amod pooled,spleen
R11214 T14441 T14439 pobj spleen,from
R11215 T14442 T14441 cc and,spleen
R11216 T14443 T14445 amod lymph,cells
R11217 T14444 T14445 compound node,cells
R11218 T14445 T14441 conj cells,spleen
R11219 T14446 T14426 punct .,purified
R11220 T14447 T14450 nummod CD8,cells
R11221 T14448 T14450 nummod +,cells
R11222 T14449 T14450 compound T,cells
R11223 T14450 T14462 nsubjpass cells,purified
R11224 T14451 T14450 prep from,cells
R11225 T14452 T14456 amod Runx3,mice
R11226 T14453 T14456 nmod −,mice
R11227 T14454 T14456 nmod /,mice
R11228 T14455 T14456 compound −,mice
R11229 T14456 T14451 pobj mice,from
R11230 T14457 T14450 prep on,cells
R11231 T14458 T14460 det the,background
R11232 T14459 T14460 compound ICR,background
R11233 T14460 T14457 pobj background,on
R11234 T14461 T14462 auxpass were,purified
R11235 T14462 T14462 ROOT purified,purified
R11236 T14463 T14462 agent by,purified
R11237 T14464 T14465 amod positive,selection
R11238 T14465 T14463 pobj selection,by
R11239 T14466 T14468 punct (,Biotec
R11240 T14467 T14468 compound Miltenyi,Biotec
R11241 T14468 T14465 appos Biotec,selection
R11242 T14469 T14468 punct ),Biotec
R11243 T14470 T14462 punct .,purified
R11244 T14471 T14472 det All,mice
R11245 T14472 T14474 nsubjpass mice,maintained
R11246 T14473 T14474 auxpass were,maintained
R11247 T14474 T14474 ROOT maintained,maintained
R11248 T14475 T14474 prep in,maintained
R11249 T14476 T14479 amod specific,facilities
R11250 T14477 T14479 amod pathogen-free,facilities
R11251 T14478 T14479 compound barrier,facilities
R11252 T14479 T14475 pobj facilities,in
R11253 T14480 T14474 cc and,maintained
R11254 T14481 T14474 conj used,maintained
R11255 T14482 T14481 prep according,used
R11256 T14483 T14482 prep to,according
R11257 T14484 T14483 pobj protocols,to
R11258 T14485 T14484 acl approved,protocols
R11259 T14486 T14485 agent by,approved
R11260 T14487 T14490 det the,Institute
R11261 T14488 T14490 compound Immune,Institute
R11262 T14489 T14490 compound Disease,Institute
R11263 T14490 T14486 pobj Institute,by
R11264 T14491 T14490 cc and,Institute
R11340 T14567 T14568 det a,concentration
R11341 T14568 T14566 pobj concentration,at
R11342 T14569 T14568 prep of,concentration
R11343 T14570 T14573 nummod 5,cells/ml
R11344 T14571 T14573 nummod ×,cells/ml
R11345 T14572 T14573 nummod 105,cells/ml
R11346 T14573 T14569 pobj cells/ml,of
R11347 T14574 T14568 prep in,concentration
R11348 T14575 T14574 pobj media,in
R11349 T14576 T14565 conj supplemented,recultured
R11350 T14577 T14576 prep with,supplemented
R11351 T14578 T14580 nummod 100,rhIL-2
R11352 T14579 T14580 compound U/ml,rhIL-2
R11353 T14580 T14577 pobj rhIL-2,with
R11354 T14581 T14559 punct .,removed
R11355 T14582 T14584 det Every,h
R11356 T14583 T14584 nummod 24,h
R11357 T14584 T14589 npadvmod h,counted
R11358 T14585 T14589 punct ",",counted
R11359 T14586 T14587 amod viable,cells
R11360 T14587 T14589 nsubjpass cells,counted
R11361 T14588 T14589 auxpass were,counted
R11362 T14589 T14589 ROOT counted,counted
R11363 T14590 T14589 cc and,counted
R11364 T14591 T14589 conj readjusted,counted
R11365 T14592 T14591 prep to,readjusted
R11366 T14593 T14596 nummod 5,cells/ml
R11367 T14594 T14596 nummod ×,cells/ml
R11368 T14595 T14596 nummod 105,cells/ml
R11369 T14596 T14592 pobj cells/ml,to
R11370 T14597 T14591 prep with,readjusted
R11371 T14598 T14599 amod fresh,media
R11372 T14599 T14597 pobj media,with
R11373 T14600 T14597 pcomp containing,with
R11374 T14601 T14603 det the,amount
R11375 T14602 T14603 amod corresponding,amount
R11376 T14603 T14600 dobj amount,containing
R11377 T14604 T14603 prep of,amount
R11378 T14605 T14604 pobj rhIL-2,of
R11379 T14606 T14589 punct .,counted
R11619 T15057 T15057 ROOT Isolation,Isolation
R11620 T15058 T15057 prep of,Isolation
R11621 T15059 T15062 nummod CD8,cells
R11622 T15060 T15062 nummod +,cells
R11623 T15061 T15062 compound T,cells
R11624 T15062 T15058 pobj cells,of
R11625 T15063 T15057 prep from,Isolation
R11626 T15064 T15068 amod Runx3,mice
R11627 T15065 T15068 compound −,mice
R11628 T15066 T15068 compound /,mice
R11629 T15067 T15068 compound −,mice
R11630 T15068 T15063 pobj mice,from
R11631 T15069 T15057 punct .,Isolation
R11632 T15070 T15072 amod Runx3-deficient,cells
R11633 T15071 T15072 compound T,cells
R11634 T15072 T15073 nsubj cells,fail
R11635 T15073 T15073 ROOT fail,fail
R11636 T15074 T15075 aux to,silence
R11637 T15075 T15073 xcomp silence,fail
R11638 T15076 T15077 compound CD4,expression
R11639 T15077 T15075 dobj expression,silence
R11640 T15078 T15075 advmod normally,silence
R11641 T15079 T15080 punct (,Fig
R11642 T15080 T15075 parataxis Fig,silence
R11643 T15081 T15073 punct .,fail
R11644 T15082 T15082 ROOT S1,S1
R11645 T15083 T15082 punct ),S1
R11646 T15084 T15087 punct (,13
R11647 T15085 T15087 nummod 12,13
R11648 T15086 T15087 punct ",",13
R11649 T15087 T15082 appos 13,S1
R11650 T15088 T15082 punct ),S1
R11651 T15089 T15082 punct .,S1
R11320 T14547 T14549 compound ml,anti
R11321 T14548 T14549 compound goat,anti
R11322 T14549 T14543 pobj anti,with
R11323 T14550 T14551 compound hamster,IgG
R11324 T14551 T14549 appos IgG,anti
R11325 T14552 T14511 punct .,cultured
R11326 T14553 T14559 prep After,removed
R11327 T14554 T14555 nummod 48,h
R11328 T14555 T14553 pobj h,After
R11329 T14556 T14559 punct ",",removed
R11330 T14557 T14559 nsubjpass cells,removed
R11331 T14558 T14559 auxpass were,removed
R11332 T14559 T14559 ROOT removed,removed
R11333 T14560 T14559 prep from,removed
R11334 T14561 T14563 det the,stimulation
R11335 T14562 T14563 compound TCR,stimulation
R11336 T14563 T14560 pobj stimulation,from
R11337 T14564 T14559 cc and,removed
R11338 T14565 T14559 conj recultured,removed
R11339 T14566 T14565 prep at,recultured
R11726 T15164 T15166 nsubjpass cells,stimulated
R11727 T15165 T15166 auxpass were,stimulated
R11728 T15166 T15166 ROOT stimulated,stimulated
R11729 T15167 T15166 prep with,stimulated
R11730 T15168 T15170 amod anti-CD3,anti-CD28
R11731 T15169 T15170 compound +,anti-CD28
R11732 T15170 T15167 pobj anti-CD28,with
R11733 T15171 T15166 prep for,stimulated
R11734 T15172 T15173 nummod 2,d
R11735 T15173 T15171 pobj d,for
R11736 T15174 T15166 prep before,stimulated
R11737 T15175 T15174 pcomp removing,before
R11738 T15176 T15175 dobj them,removing
R11739 T15177 T15175 prep from,removing
R11740 T15178 T15180 det the,stimulus
R11741 T15179 T15180 compound TCR,stimulus
R11742 T15180 T15177 pobj stimulus,from
R11743 T15181 T15175 cc and,removing
R11744 T15182 T15175 conj culturing,removing
R11745 T15183 T15182 dobj them,culturing
R11746 T15184 T15182 prep in,culturing
R11747 T15185 T15184 pobj media,in
R11748 T15186 T15185 acl containing,media
R11749 T15187 T15189 nummod 100,IL-2
R11750 T15188 T15189 compound U/ml,IL-2
R11751 T15189 T15186 dobj IL-2,containing
R11752 T15190 T15166 punct .,stimulated
R11753 T15191 T15193 mark As,reported
R11754 T15192 T15193 advmod previously,reported
R11755 T15193 T15208 advcl reported,impaired
R11756 T15194 T15208 punct ",",impaired
R11757 T15195 T15196 amod TCR-induced,proliferation
R11758 T15196 T15208 nsubjpass proliferation,impaired
R11759 T15197 T15196 prep of,proliferation
R11760 T15198 T15205 nmod Runx3,cells
R11761 T15199 T15205 nmod −,cells
R11762 T15200 T15205 nmod /,cells
R11763 T15201 T15205 nmod −,cells
R11764 T15202 T15205 nmod CD8,cells
R11765 T15203 T15205 nmod +,cells
R11766 T15204 T15205 compound T,cells
R11767 T15205 T15197 pobj cells,of
R11768 T15206 T15208 auxpass was,impaired
R11769 T15207 T15208 advmod severely,impaired
R11770 T15208 T15208 ROOT impaired,impaired
R11771 T15209 T15208 punct ",",impaired
R11772 T15210 T15208 advmod irrespective,impaired
R11773 T15211 T15210 prep of,irrespective
R11774 T15212 T15213 nummod CD4,expression
R11775 T15213 T15211 pobj expression,of
R11776 T15214 T15215 punct (,Fig
R11777 T15215 T15213 appos Fig,expression
R11778 T15216 T15208 punct .,impaired
R11779 T15217 T15217 ROOT S1,S1
R11780 T15218 T15217 punct ),S1
R11781 T15219 T15222 punct (,13
R11782 T15220 T15222 nummod 12,13
R11783 T15221 T15222 punct ",",13
R11784 T15222 T15217 appos 13,S1
R11785 T15223 T15217 punct ),S1
R11786 T15224 T15217 punct .,S1
R11787 T15225 T15230 advmod However,/
R11788 T15226 T15230 punct ",",/
R11789 T15227 T15229 det the,−
R11790 T15228 T15229 compound Runx3,−
R11791 T15229 T15230 nsubj −,/
R11652 T15090 T15093 nsubj We,fractionated
R11653 T15091 T15093 advmod therefore,fractionated
R11654 T15092 T15093 advmod further,fractionated
R11655 T15093 T15093 ROOT fractionated,fractionated
R11656 T15094 T15100 det the,cells
R11657 T15095 T15096 advmod positively,selected
R11658 T15096 T15100 amod selected,cells
R11659 T15097 T15100 compound CD8,cells
R11660 T15098 T15100 nummod +,cells
R11661 T15099 T15100 compound T,cells
R11662 T15100 T15093 dobj cells,fractionated
R11663 T15101 T15100 prep from,cells
R11664 T15102 T15103 compound Runx3,KO
R11665 T15103 T15104 compound KO,mice
R11666 T15104 T15101 pobj mice,from
R11667 T15105 T15093 prep into,fractionated
R11668 T15106 T15110 nummod CD8,SP
R11669 T15107 T15110 nummod +,SP
R11670 T15108 T15110 compound CD4,SP
R11671 T15109 T15110 compound −,SP
R11672 T15110 T15117 nmod SP,cells
R11673 T15111 T15110 cc or,SP
R11674 T15112 T15113 compound CD8,+
R11675 T15113 T15117 nmod +,cells
R11676 T15114 T15117 compound CD4,cells
R11677 T15115 T15117 compound +,cells
R11678 T15116 T15117 compound DP,cells
R11679 T15117 T15105 pobj cells,into
R11680 T15118 T15093 prep by,fractionated
R11681 T15119 T15118 pobj separation,by
R11682 T15120 T15093 advcl using,fractionated
R11683 T15121 T15123 amod anti-CD4,beads
R11684 T15122 T15123 amod magnetic,beads
R11685 T15123 T15120 dobj beads,using
R11686 T15124 T15093 punct .,fractionated
R11687 T15125 T15126 nsubj This,yielded
R11688 T15126 T15126 ROOT yielded,yielded
R11689 T15127 T15129 det a,KO
R11690 T15128 T15129 amod Runx3,KO
R11691 T15129 T15130 compound KO,SP
R11692 T15130 T15131 nsubj SP,enriched
R11693 T15131 T15132 amod enriched,population
R11694 T15132 T15126 dobj population,yielded
R11695 T15133 T15134 nsubj that,contained
R11696 T15134 T15132 relcl contained,population
R11697 T15135 T15136 nummod 75,%
R11698 T15136 T15134 dobj %,contained
R11699 T15137 T15141 nummod CD8,cells
R11700 T15138 T15141 nmod +,cells
R11701 T15139 T15141 nummod CD4,cells
R11702 T15140 T15141 compound −,cells
R11703 T15141 T15136 appos cells,%
R11704 T15142 T15141 cc and,cells
R11705 T15143 T15147 det a,population
R11706 T15144 T15145 compound KO,DP
R11707 T15145 T15147 nmod DP,population
R11708 T15146 T15147 amod enriched,population
R11709 T15147 T15141 conj population,cells
R11710 T15148 T15149 nsubj that,contained
R11711 T15149 T15147 relcl contained,population
R11712 T15150 T15151 nummod 85,%
R11713 T15151 T15149 dobj %,contained
R11714 T15152 T15156 nummod CD8,cells
R11715 T15153 T15156 nmod +,cells
R11716 T15154 T15156 nummod CD4,cells
R11717 T15155 T15156 compound +,cells
R11718 T15156 T15151 appos cells,%
R11719 T15157 T15158 punct (,Fig
R11720 T15158 T15156 appos Fig,cells
R11721 T15159 T15126 punct .,yielded
R11722 T15160 T15160 ROOT S1,S1
R11723 T15161 T15160 punct ),S1
R11724 T15162 T15160 punct .,S1
R11725 T15163 T15164 det The,cells
R11801 T15239 T15240 amod activated,cells
R11802 T15240 T15238 pobj cells,of
R11803 T15241 T15240 punct ",",cells
R11804 T15242 T15240 prep including,cells
R11805 T15243 T15242 pobj up-regulation,including
R11806 T15244 T15243 prep of,up-regulation
R11807 T15245 T15244 pobj CD25,of
R11808 T15246 T15245 cc and,CD25
R11809 T15247 T15245 conj CD69,CD25
R11810 T15248 T15249 punct (,Fig
R11811 T15249 T15247 appos Fig,CD69
R11812 T15250 T15230 punct .,/
R11813 T15251 T15251 ROOT S1,S1
R11814 T15252 T15251 punct ),S1
R11815 T15253 T15251 punct .,S1
R11816 T15254 T15255 mark As,expected
R11817 T15255 T15271 advcl expected,responded
R11818 T15256 T15255 prep from,expected
R11819 T15257 T15258 poss their,ability
R11820 T15258 T15256 pobj ability,from
R11821 T15259 T15260 aux to,up-regulate
R11822 T15260 T15258 acl up-regulate,ability
R11823 T15261 T15260 dobj CD25,up-regulate
R11824 T15262 T15261 punct ",",CD25
R11825 T15263 T15271 nsubj Runx3,responded
R11826 T15264 T15270 nmod −,cells
R11827 T15265 T15270 nmod /,cells
R11828 T15266 T15270 nmod −,cells
R11829 T15267 T15270 nmod CD8,cells
R11830 T15268 T15270 nmod +,cells
R11831 T15269 T15270 compound T,cells
R11832 T15270 T15271 nsubj cells,responded
R11833 T15271 T15271 ROOT responded,responded
R11834 T15272 T15271 prep to,responded
R11835 T15273 T15272 pobj IL-2,to
R11836 T15274 T15271 dobj supplementation,responded
R11837 T15275 T15271 prep after,responded
R11838 T15276 T15275 pobj day,after
R11839 T15277 T15276 nummod 2,day
R11840 T15278 T15271 cc and,responded
R11841 T15279 T15280 advmod efficiently,expanded
R11842 T15280 T15271 conj expanded,responded
R11843 T15281 T15280 prep until,expanded
R11844 T15282 T15281 pobj day,until
R11845 T15283 T15282 nummod 6,day
R11846 T15284 T15282 prep of,day
R11847 T15285 T15287 det the,period
R11848 T15286 T15287 compound culture,period
R11849 T15287 T15284 pobj period,of
R11850 T15288 T15282 punct ",",day
R11851 T15289 T15280 advmod albeit,expanded
R11852 T15290 T15280 prep at,expanded
R11853 T15291 T15292 amod slower,rates
R11854 T15292 T15290 pobj rates,at
R11855 T15293 T15271 prep compared,responded
R11856 T15294 T15293 prep with,compared
R11857 T15295 T15296 compound WT,cells
R11858 T15296 T15294 pobj cells,with
R11859 T15297 T15298 punct (,Fig
R11860 T15298 T15296 appos Fig,cells
R11861 T15299 T15271 punct .,responded
R11862 T15300 T15300 ROOT S1,S1
R11863 T15301 T15300 punct ),S1
R11864 T15302 T15300 punct .,S1
R11865 T15303 T15311 mark Although,silenced
R11866 T15304 T15305 det a,fraction
R11867 T15305 T15311 nsubj fraction,silenced
R11868 T15306 T15305 prep of,fraction
R11869 T15307 T15310 det the,cells
R11870 T15308 T15310 compound KO,cells
R11871 T15309 T15310 compound DP,cells
R11872 T15310 T15306 pobj cells,of
R11873 T15311 T15326 advcl silenced,remained
R11874 T15312 T15313 nummod CD4,expression
R11875 T15313 T15311 dobj expression,silenced
R11876 T15314 T15311 prep after,silenced
R11877 T15315 T15314 pobj activation,after
R11878 T15316 T15326 punct ",",remained
R11879 T15317 T15318 det the,ratio
R11880 T15318 T15326 nsubj ratio,remained
R11881 T15319 T15318 prep of,ratio
R11882 T15320 T15321 compound SP/DP,cells
R11883 T15321 T15319 pobj cells,of
R11884 T15322 T15318 prep in,ratio
R11792 T15230 T15230 ROOT /,/
R11793 T15231 T15232 nummod −,cells
R11794 T15232 T15233 nsubj cells,showed
R11795 T15233 T15230 ccomp showed,/
R11796 T15234 T15236 amod cell-surface,patterns
R11797 T15235 T15236 compound expression,patterns
R11798 T15236 T15233 dobj patterns,showed
R11799 T15237 T15236 amod indicative,patterns
R11800 T15238 T15237 prep of,indicative
R11951 T15389 T15388 prep from,are
R11952 T15390 T15391 amod Runx3,KO
R11953 T15391 T15393 compound KO,cells
R11954 T15392 T15393 compound SP,cells
R11955 T15393 T15389 pobj cells,from
R11956 T15394 T15388 punct ",",are
R11957 T15395 T15403 mark whereas,are
R11958 T15396 T15403 nsubj those,are
R11959 T15397 T15396 acl shown,those
R11960 T15398 T15397 prep in,shown
R11961 T15399 T15398 pobj Figs.,in
R11962 T15400 T15399 nummod 3,Figs.
R11963 T15401 T15397 cc and,shown
R11964 T15402 T15403 nsubj 4,are
R11965 T15403 T15388 advcl are,are
R11966 T15404 T15403 prep from,are
R11967 T15405 T15407 amod total,KO
R11968 T15406 T15407 compound Runx3,KO
R11969 T15407 T15409 compound KO,cells
R11970 T15408 T15409 compound CD8,cells
R11971 T15409 T15404 pobj cells,from
R11972 T15410 T15388 punct .,are
R12352 T16130 T16132 amod FACS-based,assay
R12353 T16131 T16132 compound cytotoxicity,assay
R12354 T16132 T16132 ROOT assay,assay
R12355 T16133 T16132 punct .,assay
R12356 T16134 T16135 aux To,measure
R12357 T16135 T16143 advcl measure,loaded
R12358 T16136 T16135 dobj cytotoxicity,measure
R12359 T16137 T16143 punct ",",loaded
R12360 T16138 T16141 nummod EL4,cells
R12361 T16139 T16141 compound thymoma,cells
R12362 T16140 T16141 compound target,cells
R12363 T16141 T16143 nsubjpass cells,loaded
R12364 T16142 T16143 auxpass were,loaded
R12365 T16143 T16143 ROOT loaded,loaded
R12366 T16144 T16143 prep with,loaded
R12367 T16145 T16144 pobj 0,with
R12368 T16146 T16145 cc or,0
R12369 T16147 T16145 conj 1,0
R12370 T16148 T16150 compound μM,peptide
R12371 T16149 T16150 compound Gp33,peptide
R12372 T16150 T16145 appos peptide,0
R12373 T16151 T16150 prep for,peptide
R12374 T16152 T16153 nummod 2,h
R12375 T16153 T16151 pobj h,for
R12376 T16154 T16143 prep before,loaded
R12377 T16155 T16157 det a,coincubation
R12378 T16156 T16157 amod 2-h,coincubation
R12379 T16157 T16154 pobj coincubation,before
R12380 T16158 T16157 prep with,coincubation
R12381 T16159 T16160 compound P14,CD8
R12382 T16160 T16158 pobj CD8,with
R12383 T16161 T16143 advcl +,loaded
R12384 T16162 T16163 compound T,cells
R12385 T16163 T16161 dobj cells,+
R12386 T16164 T16161 prep at,+
R12387 T16165 T16167 det the,ratios
R12388 T16166 T16167 amod effector-to-target,ratios
R12389 T16167 T16164 pobj ratios,at
R12390 T16168 T16167 acl indicated,ratios
R12391 T16169 T16168 prep in,indicated
R12392 T16170 T16171 det the,figures
R12393 T16171 T16169 pobj figures,in
R12394 T16172 T16171 prep in,figures
R12395 T16173 T16175 amod 96-well,plates
R12396 T16174 T16175 compound round-bottom,plates
R12397 T16175 T16172 pobj plates,in
R12398 T16176 T16143 punct .,loaded
R12399 T16177 T16184 prep After,stained
R12400 T16178 T16180 det the,period
R12401 T16179 T16180 compound coincubation,period
R12402 T16180 T16177 pobj period,After
R12403 T16181 T16184 punct ",",stained
R12629 T16469 T16470 det the,antibodies
R12630 T16470 T16468 dobj antibodies,containing
R12631 T16471 T16441 conj indicated,washed
R12632 T16472 T16471 prep in,indicated
R12633 T16473 T16474 det the,figures
R12634 T16474 T16472 pobj figures,in
R11885 T15323 T15325 det each,population
R11886 T15324 T15325 amod enriched,population
R11887 T15325 T15322 pobj population,in
R11888 T15326 T15326 ROOT remained,remained
R11889 T15327 T15326 acomp constant,remained
R11890 T15328 T15326 advmod thereafter,remained
R11891 T15329 T15326 punct ",",remained
R11892 T15330 T15326 cc and,remained
R11893 T15331 T15334 nsubj we,observe
R11894 T15332 T15334 aux did,observe
R11895 T15333 T15334 neg not,observe
R11896 T15334 T15326 conj observe,remained
R11897 T15335 T15337 det any,differences
R11898 T15336 T15337 amod major,differences
R11899 T15337 T15334 dobj differences,observe
R11900 T15338 T15337 prep between,differences
R11901 T15339 T15341 det these,populations
R11902 T15340 T15341 nummod two,populations
R11903 T15341 T15338 pobj populations,between
R11904 T15342 T15334 prep throughout,observe
R11905 T15343 T15345 det the,period
R11906 T15344 T15345 compound culture,period
R11907 T15345 T15342 pobj period,throughout
R11908 T15346 T15334 punct ",",observe
R11909 T15347 T15334 advcl indicating,observe
R11910 T15348 T15373 mark that,are
R11911 T15349 T15373 prep in,are
R11912 T15350 T15349 pobj terms,in
R11913 T15351 T15350 prep of,terms
R11914 T15352 T15354 compound effector,differentiation
R11915 T15353 T15354 compound CTL,differentiation
R11916 T15354 T15351 pobj differentiation,of
R11917 T15355 T15349 cc and,in
R11918 T15356 T15349 conj under,in
R11919 T15357 T15359 poss our,conditions
R11920 T15358 T15359 compound culture,conditions
R11921 T15359 T15356 pobj conditions,under
R11922 T15360 T15368 punct ",",cells
R11923 T15361 T15368 amod Runx3,cells
R11924 T15362 T15366 nmod −,+
R11925 T15363 T15366 nmod /,+
R11926 T15364 T15366 nmod −,+
R11927 T15365 T15366 nmod CD8,+
R11928 T15366 T15368 nmod +,cells
R11929 T15367 T15368 compound T,cells
R11930 T15368 T15373 nsubj cells,are
R11931 T15369 T15371 nsubj that,coexpress
R11932 T15370 T15371 advmod also,coexpress
R11933 T15371 T15368 relcl coexpress,cells
R11934 T15372 T15371 dobj CD4,coexpress
R11935 T15373 T15347 ccomp are,indicating
R11936 T15374 T15373 acomp indistinguishable,are
R11937 T15375 T15374 prep from,indistinguishable
R11938 T15376 T15375 pobj those,from
R11939 T15377 T15378 nsubj that,do
R11940 T15378 T15376 relcl do,those
R11941 T15379 T15378 neg not,do
R11942 T15380 T15334 punct .,observe
R11943 T15381 T15382 det The,data
R11944 T15382 T15382 ROOT data,data
R11945 T15383 T15382 acl presented,data
R11946 T15384 T15383 prep in,presented
R11947 T15385 T15384 pobj Fig,in
R11948 T15386 T15383 punct .,presented
R11949 T15387 T15388 nsubj S2,are
R11950 T15388 T15388 ROOT are,are
R12404 T16182 T16184 nsubjpass cells,stained
R12405 T16183 T16184 auxpass were,stained
R12406 T16184 T16184 ROOT stained,stained
R12407 T16185 T16184 prep with,stained
R12408 T16186 T16187 compound Annexin,V
R12409 T16187 T16185 pobj V,with
R12410 T16188 T16187 conj FITC,V
R12411 T16189 T16188 cc and,FITC
R12412 T16190 T16188 conj anti-CD8,FITC
R12413 T16191 T16184 npadvmod allophycocyanin,stained
R12414 T16192 T16184 punct .,stained
R12415 T16193 T16194 compound Data,analysis
R12416 T16194 T16196 nsubjpass analysis,performed
R12417 T16195 T16196 auxpass was,performed
R12418 T16196 T16214 ccomp performed,were
R12419 T16197 T16196 prep with,performed
R12420 T16198 T16199 compound FlowJo,software
R12421 T16199 T16197 pobj software,with
R12422 T16200 T16199 punct (,software
R12423 T16201 T16202 compound Tree,Star
R12424 T16202 T16199 appos Star,software
R12425 T16203 T16204 punct ",",Inc.
R12426 T16204 T16199 appos Inc.,software
R12427 T16205 T16196 punct ),performed
R12428 T16206 T16214 punct ;,were
R12429 T16207 T16209 nummod EL4,cells
R12430 T16208 T16209 compound target,cells
R12431 T16209 T16214 nsubj cells,were
R12432 T16210 T16209 punct (,cells
R12433 T16211 T16212 amod CD8-negative,events
R12434 T16212 T16209 appos events,cells
R12435 T16213 T16209 punct ),cells
R12436 T16214 T16214 ROOT were,were
R12437 T16215 T16214 acomp gated,were
R12438 T16216 T16214 punct ",",were
R12439 T16217 T16214 cc and,were
R12440 T16218 T16219 det the,percentage
R12441 T16219 T16227 nsubjpass percentage,determined
R12442 T16220 T16219 prep of,percentage
R12443 T16221 T16222 compound Annexin,V
R12444 T16222 T16220 pobj V,of
R12445 T16223 T16225 amod +,cells
R12446 T16224 T16225 compound target,cells
R12447 T16225 T16219 conj cells,percentage
R12448 T16226 T16227 auxpass was,determined
R12449 T16227 T16214 conj determined,were
R12450 T16228 T16227 punct .,determined
R12545 T16385 T16385 ROOT Cytokine,Cytokine
R12546 T16386 T16385 cc and,Cytokine
R12547 T16387 T16388 compound surface,marker
R12548 T16388 T16385 conj marker,Cytokine
R12549 T16389 T16385 acl staining,Cytokine
R12550 T16390 T16385 punct .,Cytokine
R12551 T16391 T16392 aux To,assess
R12552 T16392 T16398 advcl assess,restimulated
R12553 T16393 T16394 amod cytokine,production
R12554 T16394 T16392 dobj production,assess
R12555 T16395 T16398 punct ",",restimulated
R12556 T16396 T16398 nsubjpass cells,restimulated
R12557 T16397 T16398 auxpass were,restimulated
R12558 T16398 T16398 ROOT restimulated,restimulated
R12559 T16399 T16398 prep with,restimulated
R12560 T16400 T16403 nummod 10,+
R12561 T16401 T16402 compound nM,PMA
R12562 T16402 T16403 nsubj PMA,+
R12563 T16403 T16398 advcl +,restimulated
R12564 T16404 T16405 nummod 1,μM
R12565 T16405 T16406 compound μM,ionomycin
R12566 T16406 T16403 dobj ionomycin,+
R12567 T16407 T16403 prep for,+
R12568 T16408 T16409 nummod 6,h
R12569 T16409 T16407 pobj h,for
R12570 T16410 T16412 punct (,indicated
R12571 T16411 T16412 mark unless,indicated
R12572 T16412 T16403 advcl indicated,+
R12573 T16413 T16412 advmod otherwise,indicated
R12574 T16414 T16412 prep in,indicated
R12575 T16415 T16416 det the,figures
R12576 T16416 T16414 pobj figures,in
R12577 T16417 T16412 punct ),indicated
R12578 T16418 T16412 punct ",",indicated
R12579 T16419 T16412 cc and,indicated
R12580 T16420 T16422 amod intracellular,stains
R12581 T16421 T16422 compound cytokine,stains
R12582 T16422 T16424 nsubjpass stains,performed
R12583 T16423 T16424 auxpass were,performed
R12584 T16424 T16412 conj performed,indicated
R12585 T16425 T16427 mark as,described
R12586 T16426 T16427 advmod previously,described
R12587 T16427 T16424 advcl described,performed
R12588 T16428 T16427 punct (,described
R12589 T16429 T16427 npadvmod 28,described
R12635 T16475 T16471 prep at,indicated
R12636 T16476 T16477 advmod previously,optimized
R12637 T16477 T16478 amod optimized,concentrations
R12638 T16478 T16475 pobj concentrations,at
R12639 T16479 T16471 punct ",",indicated
R12640 T16480 T16491 nsubj incubated,washed
R12641 T16481 T16480 prep for,incubated
R12642 T16482 T16483 nummod 15,min
R12643 T16483 T16481 pobj min,for
R12644 T16484 T16483 prep at,min
R12645 T16485 T16486 compound room,temperature
R12646 T16486 T16484 pobj temperature,at
R12647 T16487 T16488 punct (,RT
R12648 T16488 T16483 parataxis RT,min
R12649 T16489 T16488 punct ),RT
R12650 T16490 T16483 punct ",",min
R12651 T16491 T16471 conj washed,indicated
R12652 T16492 T16491 punct ",",washed
R12653 T16493 T16491 cc and,washed
R12654 T16494 T16491 conj resuspended,washed
R12655 T16495 T16494 prep in,resuspended
R12656 T16496 T16497 nummod 2,%
R12657 T16497 T16500 nmod %,solution
R12658 T16498 T16500 nmod formaldehyde,solution
R12659 T16499 T16500 amod fixative,solution
R12660 T16500 T16495 pobj solution,in
R12661 T16501 T16500 prep before,solution
R12662 T16502 T16501 pobj acquisition,before
R12663 T16503 T16502 prep on,acquisition
R12664 T16504 T16505 det a,FACSCalibur
R12665 T16505 T16503 pobj FACSCalibur,on
R12666 T16506 T16507 punct (,BD
R12667 T16507 T16505 appos BD,FACSCalibur
R12668 T16508 T16505 punct ),FACSCalibur
R12669 T16509 T16441 punct .,washed
R12858 T16715 T16714 cc and,removed
R12859 T16716 T16714 conj replaced,removed
R12860 T16717 T16716 prep with,replaced
R12861 T16718 T16719 amod complete,media
R12862 T16719 T16717 pobj media,with
R12863 T16720 T16714 conj supplemented,removed
R12864 T16721 T16720 prep with,supplemented
R12865 T16722 T16723 nummod 8,μg
R12866 T16723 T16726 nmod μg,polybrene
R12867 T16724 T16726 nmod /,polybrene
R12868 T16725 T16726 compound ml,polybrene
R12869 T16726 T16721 pobj polybrene,with
R12870 T16727 T16726 acl containing,polybrene
R12871 T16728 T16731 amod fresh,virus
R12872 T16729 T16728 cc plus,fresh
R12873 T16730 T16728 conj concentrated,fresh
R12874 T16731 T16727 dobj virus,containing
R12875 T16732 T16714 punct .,removed
R12876 T16733 T16734 det The,plates
R12877 T16734 T16736 nsubjpass plates,centrifuged
R12878 T16735 T16736 auxpass were,centrifuged
R12879 T16736 T16736 ROOT centrifuged,centrifuged
R12880 T16737 T16736 prep at,centrifuged
R12881 T16738 T16739 nummod 700,g
R12882 T16739 T16737 pobj g,at
R12883 T16740 T16736 prep for,centrifuged
R12884 T16741 T16742 nummod 1,h
R12885 T16742 T16740 pobj h,for
R12886 T16743 T16742 prep at,h
R12887 T16744 T16743 pobj RT,at
R12888 T16745 T16736 prep before,centrifuged
R12889 T16746 T16745 pcomp returning,before
R12890 T16747 T16746 prep to,returning
R12891 T16748 T16749 compound 37,°
R12892 T16749 T16750 nummod °,C
R12893 T16750 T16747 pobj C,to
R12894 T16751 T16746 prep for,returning
R12895 T16752 T16757 det an,constructs
R12896 T16753 T16757 amod additional,constructs
R12897 T16754 T16755 nummod 5,h.
R12898 T16755 T16756 compound h.,Retroviral
R12899 T16756 T16757 compound Retroviral,constructs
R12900 T16757 T16751 pobj constructs,for
R12901 T16758 T16757 prep for,constructs
R12902 T16759 T16758 pobj Eomes-VP16,for
R12903 T16760 T16759 cc and,Eomes-VP16
R12904 T16761 T16765 det the,vector
R12905 T16762 T16763 compound MIG,control
R12906 T16763 T16765 nmod control,vector
R12907 T16764 T16765 amod empty,vector
R12908 T16765 T16759 conj vector,Eomes-VP16
R12909 T16766 T16736 auxpass were,centrifuged
R12910 T16767 T16768 det a,gift
R12911 T16768 T16736 nsubjpass gift,centrifuged
R12912 T16769 T16768 prep from,gift
R12913 T16770 T16771 compound S.L.,Reiner
R12914 T16771 T16769 pobj Reiner,from
R12915 T16772 T16771 punct (,Reiner
R12590 T16430 T16427 punct ),described
R12591 T16431 T16403 punct .,+
R12592 T16432 T16433 aux To,detect
R12593 T16433 T16441 advcl detect,washed
R12594 T16434 T16433 dobj expression,detect
R12595 T16435 T16434 prep of,expression
R12596 T16436 T16437 compound surface,molecules
R12597 T16437 T16435 pobj molecules,of
R12598 T16438 T16441 punct ",",washed
R12599 T16439 T16441 nsubjpass cells,washed
R12600 T16440 T16441 auxpass were,washed
R12601 T16441 T16441 ROOT washed,washed
R12602 T16442 T16441 prep in,washed
R12603 T16443 T16442 pobj PBS,in
R12604 T16444 T16441 punct ",",washed
R12605 T16445 T16441 conj resuspended,washed
R12606 T16446 T16448 mark in,wash
R12607 T16447 T16448 nsubj FACS,wash
R12608 T16448 T16471 meta wash,indicated
R12609 T16449 T16448 dobj buffer,wash
R12610 T16450 T16453 punct (,FBS
R12611 T16451 T16452 nummod 3,%
R12612 T16452 T16453 npadvmod %,FBS
R12613 T16453 T16471 nsubj FBS,indicated
R12614 T16454 T16453 punct ",",FBS
R12615 T16455 T16456 nummod 0.1,%
R12616 T16456 T16458 compound %,azide
R12617 T16457 T16458 compound sodium,azide
R12618 T16458 T16453 appos azide,FBS
R12619 T16459 T16458 punct ",",azide
R12620 T16460 T16462 nummod 30,Hepes
R12621 T16461 T16462 compound mM,Hepes
R12622 T16462 T16458 appos Hepes,azide
R12623 T16463 T16462 punct ",",Hepes
R12624 T16464 T16466 nummod 1,PBS
R12625 T16465 T16466 nummod ×,PBS
R12626 T16466 T16462 appos PBS,Hepes
R12627 T16467 T16462 punct ),Hepes
R12628 T16468 T16458 acl containing,azide
R12799 T16656 T16657 compound Retroviral,transduction
R12800 T16657 T16657 ROOT transduction,transduction
R12801 T16658 T16657 prep of,transduction
R12802 T16659 T16663 amod primary,cells
R12803 T16660 T16663 nmod CD8,cells
R12804 T16661 T16663 nummod +,cells
R12805 T16662 T16663 compound T,cells
R12806 T16663 T16658 pobj cells,of
R12807 T16664 T16657 punct .,transduction
R12808 T16665 T16672 prep For,generated
R12809 T16666 T16667 compound transduction,experiments
R12810 T16667 T16665 pobj experiments,For
R12811 T16668 T16672 punct ",",generated
R12812 T16669 T16670 amod viral,supernatants
R12813 T16670 T16672 nsubjpass supernatants,generated
R12814 T16671 T16672 auxpass were,generated
R12815 T16672 T16672 ROOT generated,generated
R12816 T16673 T16672 prep by,generated
R12817 T16674 T16676 compound calcium,transfection
R12818 T16675 T16676 compound phosphate,transfection
R12819 T16676 T16673 pobj transfection,by
R12820 T16677 T16676 prep of,transfection
R12821 T16678 T16679 compound Phoenix,cells
R12822 T16679 T16677 pobj cells,of
R12823 T16680 T16679 cc and,cells
R12824 T16681 T16676 conj concentration,transfection
R12825 T16682 T16681 prep by,concentration
R12826 T16683 T16684 amod overnight,centrifugation
R12827 T16684 T16682 pobj centrifugation,by
R12828 T16685 T16672 prep at,generated
R12829 T16686 T16687 nummod "6,000",g
R12830 T16687 T16685 pobj g,at
R12831 T16688 T16672 punct .,generated
R12832 T16689 T16714 prep At,removed
R12833 T16690 T16714 nsubjpass ∼,removed
R12834 T16691 T16692 nummod 42,h
R12835 T16692 T16693 npadvmod h,after
R12836 T16693 T16690 prep after,∼
R12837 T16694 T16697 det the,activation
R12838 T16695 T16697 amod initial,activation
R12839 T16696 T16697 compound TCR,activation
R12840 T16697 T16693 pobj activation,after
R12841 T16698 T16697 prep of,activation
R12842 T16699 T16703 nummod 106,cells
R12843 T16700 T16703 nummod CD8,cells
R12844 T16701 T16703 compound +,cells
R12845 T16702 T16703 compound T,cells
R12846 T16703 T16698 pobj cells,of
R12847 T16704 T16703 prep per,cells
R12848 T16705 T16706 advmod well,in
R12849 T16706 T16704 prep in,per
R12850 T16707 T16708 amod 12-well,plates
R12851 T16708 T16706 pobj plates,in
R12852 T16709 T16714 punct ",",removed
R12853 T16710 T16712 det the,media
R12854 T16711 T16712 compound culture,media
R12855 T16712 T16714 nsubjpass media,removed
R12856 T16713 T16714 auxpass was,removed
R12857 T16714 T16714 ROOT removed,removed
R13635 T17622 T17611 relcl confirmed,membranes
R13636 T17623 T17622 agent by,confirmed
R13637 T17624 T17626 amod ethidium,staining
R13638 T17625 T17626 compound bromide,staining
R13639 T17626 T17623 pobj staining,by
R13640 T17627 T17626 prep of,staining
R13641 T17628 T17630 amod ribosomal,species
R13642 T17629 T17630 compound RNA,species
R13643 T17630 T17627 pobj species,of
R13644 T17631 T17626 prep on,staining
R13645 T17632 T17633 det the,membrane
R13646 T17633 T17631 pobj membrane,on
R13647 T17634 T17602 punct .,loaded
R13648 T17635 T17637 nsubjpass Membranes,hybridized
R13649 T17636 T17637 auxpass were,hybridized
R13650 T17637 T17646 ccomp hybridized,dCTP
R13651 T17638 T17637 prep with,hybridized
R13652 T17639 T17640 nummod 1,ng/ml
R13653 T17640 T17638 pobj ng/ml,with
R13654 T17641 T17637 punct α,hybridized
R13655 T17642 T17646 punct -,dCTP
R13656 T17643 T17646 compound [,dCTP
R13657 T17644 T17646 compound 32P,dCTP
R13658 T17645 T17646 compound ],dCTP
R13659 T17646 T17646 ROOT dCTP,dCTP
R13660 T17647 T17646 acl labeled,dCTP
R13661 T17648 T17649 amod trichloroacetic,acid
R13662 T17649 T17651 nmod acid,probe
R13663 T17650 T17651 amod precipitable,probe
R13664 T17651 T17647 dobj probe,labeled
R13665 T17652 T17647 prep in,labeled
R13666 T17653 T17655 nmod ExpressHyb,buffer
R13667 T17654 T17655 compound hybridization,buffer
R13668 T17655 T17652 pobj buffer,in
R13669 T17656 T17658 punct (,Laboratories
R13670 T17657 T17658 compound Clontech,Laboratories
R13671 T17658 T17655 appos Laboratories,buffer
R13672 T17659 T17658 punct ",",Laboratories
R13673 T17660 T17658 appos Inc.,Laboratories
R13674 T17661 T17658 punct ),Laboratories
R13675 T17662 T17646 punct .,dCTP
R13676 T17663 T17665 det All,probes
R13677 T17664 T17665 compound cDNA,probes
R13678 T17665 T17667 nsubjpass probes,confirmed
R13679 T17666 T17667 auxpass were,confirmed
R13680 T17667 T17667 ROOT confirmed,confirmed
R13681 T17668 T17669 aux to,have
R13682 T17669 T17667 xcomp have,confirmed
R13683 T17670 T17673 det the,specificity
R13684 T17671 T17673 amod appropriate,specificity
R13685 T17672 T17673 amod single-copy,specificity
R13686 T17673 T17669 dobj specificity,have
R13687 T17674 T17669 prep under,have
R13688 T17675 T17676 det these,conditions
R13689 T17676 T17674 pobj conditions,under
R13690 T17677 T17669 advcl using,have
R13691 T17678 T17681 amod genomic,analysis
R13692 T17679 T17680 compound Southern,blot
R13693 T17680 T17681 compound blot,analysis
R12916 T16773 T16771 appos University,Reiner
R12917 T16774 T16773 prep of,University
R12918 T16775 T16774 pobj Pennsylvania,of
R12919 T16776 T16775 punct ",",Pennsylvania
R12920 T16777 T16775 conj Philadelphia,Pennsylvania
R12921 T16778 T16777 punct ",",Philadelphia
R12922 T16779 T16777 conj PA,Philadelphia
R12923 T16780 T16771 punct ),Reiner
R12924 T16781 T16771 punct (,Reiner
R12925 T16782 T16771 appos 8,Reiner
R12926 T16783 T16771 punct ),Reiner
R12927 T16784 T16736 punct .,centrifuged
R13790 T17777 T17775 pobj antibodies,with
R13791 T17778 T17774 conj indicated,performed
R13792 T17779 T17778 prep in,indicated
R13793 T17780 T17781 det the,figures
R13794 T17781 T17779 pobj figures,in
R13795 T17782 T17792 mark after,loaded
R13796 T17783 T17782 pobj SDS-PAGE,after
R13797 T17784 T17783 punct (,SDS-PAGE
R13798 T17785 T17787 nummod 10,μg
R13799 T17786 T17787 nummod 30,μg
R13800 T17787 T17783 appos μg,SDS-PAGE
R13801 T17788 T17787 prep of,μg
R13802 T17789 T17790 amod total,protein
R13803 T17790 T17788 pobj protein,of
R13804 T17791 T17792 auxpass was,loaded
R13805 T17792 T17778 advcl loaded,indicated
R13806 T17793 T17792 prep per,loaded
R13807 T17794 T17793 pobj well,per
R13808 T17795 T17778 punct ),indicated
R13809 T17796 T17774 punct .,performed
R13810 T17797 T17802 nsubjpass Quantification,performed
R13811 T17798 T17797 prep of,Quantification
R13812 T17799 T17800 amod detected,protein
R13813 T17800 T17798 pobj protein,of
R13814 T17801 T17802 auxpass was,performed
R13815 T17802 T17802 ROOT performed,performed
R13816 T17803 T17802 prep with,performed
R13817 T17804 T17808 det an,unit
R13818 T17805 T17807 compound Intelligent,Box
R13819 T17806 T17807 compound Dark,Box
R13820 T17807 T17808 compound Box,unit
R13821 T17808 T17803 pobj unit,with
R13822 T17809 T17808 punct (,unit
R13823 T17810 T17808 appos LAS-3000,unit
R13824 T17811 T17812 punct ;,Fujifilm
R13825 T17812 T17802 npadvmod Fujifilm,performed
R13826 T17813 T17812 punct ),Fujifilm
R13827 T17814 T17802 cc and,performed
R13828 T17815 T17802 conj normalized,performed
R13829 T17816 T17815 prep for,normalized
R13830 T17817 T17816 pcomp loading,for
R13831 T17818 T17817 prep with,loading
R13832 T17819 T17820 det the,amount
R13833 T17820 T17818 pobj amount,with
R13834 T17821 T17820 prep of,amount
R13835 T17822 T17823 compound RNA,Pol-II
R13836 T17823 T17821 pobj Pol-II,of
R13837 T17824 T17820 acl detected,amount
R13838 T17825 T17824 prep in,detected
R13839 T17826 T17827 det each,lane
R13840 T17827 T17825 pobj lane,in
R13841 T17828 T17802 punct .,performed
R14302 T18430 T18431 compound mRNA,amounts
R14303 T18431 T18428 pobj amounts,of
R14304 T18432 T18427 prep by,Quantification
R14305 T18433 T18434 compound phosphorimager,analysis
R14306 T18434 T18432 pobj analysis,by
R14307 T18435 T18427 punct .,Quantification
R14308 T18436 T18437 punct (,C
R14309 T18437 T18439 nmod C,Kinetics
R14310 T18438 T18437 punct ),C
R14311 T18439 T18439 ROOT Kinetics,Kinetics
R14312 T18440 T18439 prep of,Kinetics
R14313 T18441 T18442 compound protein,expression
R14314 T18442 T18440 pobj expression,of
R14315 T18443 T18439 prep in,Kinetics
R14316 T18444 T18443 pcomp differentiating,in
R14317 T18445 T18446 compound P14,CD8
R14318 T18446 T18444 dobj CD8,differentiating
R14319 T18447 T18449 amod +,cells
R14320 T18448 T18449 compound T,cells
R14321 T18449 T18444 dobj cells,differentiating
R14338 T18466 T18466 ROOT amounts,amounts
R14339 T18467 T18466 acl quantified,amounts
R14340 T18468 T18467 prep from,quantified
R14341 T18469 T18471 det the,blots
R14342 T18470 T18471 amod Western,blots
R14343 T18471 T18468 pobj blots,from
R14344 T18472 T18466 punct .,amounts
R14345 T18473 T18476 punct (,Intracellular
R14346 T18474 T18476 nmod E,Intracellular
R14347 T18475 T18476 punct ),Intracellular
R14348 T18476 T18476 ROOT Intracellular,Intracellular
R14349 T18477 T18476 acl staining,Intracellular
R14350 T18478 T18477 prep for,staining
R13694 T17681 T17677 dobj analysis,using
R13695 T17682 T17667 punct .,confirmed
R13696 T17683 T17684 compound Band,intensities
R13697 T17684 T17686 nsubjpass intensities,acquired
R13698 T17685 T17686 auxpass were,acquired
R13699 T17686 T17686 ROOT acquired,acquired
R13700 T17687 T17686 agent by,acquired
R13701 T17688 T17689 amod phosphorimaging,analysis
R13702 T17689 T17687 pobj analysis,by
R13703 T17690 T17686 punct .,acquired
R13704 T17691 T17699 prep For,obtained
R13705 T17692 T17693 amod Western,analysis
R13706 T17693 T17691 pobj analysis,For
R13707 T17694 T17699 punct ",",obtained
R13708 T17695 T17697 amod whole-cell,lysates
R13709 T17696 T17697 compound protein,lysates
R13710 T17697 T17699 nsubjpass lysates,obtained
R13711 T17698 T17699 auxpass were,obtained
R13712 T17699 T17699 ROOT obtained,obtained
R13732 T17719 T17716 pobj IL-2,in
R13733 T17720 T17719 prep with,IL-2
R13749 T17736 T17737 nummod 10,%
R13750 T17737 T17738 compound %,glycerol
R13751 T17738 T17734 appos glycerol,NaCl
R13752 T17739 T17734 punct ",",NaCl
R13753 T17740 T17742 nummod 5,EDTA
R13754 T17741 T17742 compound mM,EDTA
R13755 T17742 T17734 appos EDTA,NaCl
R13756 T17743 T17742 punct ",",EDTA
R13757 T17744 T17745 nummod 1,%
R13758 T17745 T17746 compound %,NP-40
R13759 T17746 T17742 appos NP-40,EDTA
R13760 T17747 T17742 punct ),EDTA
R13761 T17748 T17709 prep by,indicated
R13783 T17770 T17699 punct .,obtained
R13784 T17771 T17772 compound Immunoblot,analysis
R13785 T17772 T17774 nsubjpass analysis,performed
R13786 T17773 T17774 auxpass was,performed
R13787 T17774 T17774 ROOT performed,performed
R13788 T17775 T17774 prep with,performed
R13789 T17776 T17777 det the,antibodies
R14094 T18126 T18128 amod Online,material
R14095 T18127 T18128 amod supplemental,material
R14096 T18128 T18128 ROOT material,material
R14097 T18129 T18128 punct .,material
R14098 T18130 T18130 ROOT Fig,Fig
R14099 T18131 T18130 punct .,Fig
R14100 T18132 T18133 nsubj S1,shows
R14101 T18133 T18133 ROOT shows,shows
R14102 T18134 T18135 det the,characterization
R14103 T18135 T18133 dobj characterization,shows
R14104 T18136 T18135 prep of,characterization
R14105 T18137 T18141 amod peripheral,cells
R14106 T18138 T18141 nmod CD8,cells
R14107 T18139 T18141 nummod +,cells
R14108 T18140 T18141 compound T,cells
R14109 T18141 T18136 pobj cells,of
R14110 T18142 T18135 prep from,characterization
R14111 T18143 T18147 amod Runx3,mice
R14112 T18144 T18147 compound −,mice
R14113 T18145 T18147 compound /,mice
R14114 T18146 T18147 compound −,mice
R14115 T18147 T18142 pobj mice,from
R14116 T18148 T18133 punct .,shows
R14117 T18149 T18149 ROOT Fig,Fig
R14118 T18150 T18149 punct .,Fig
R14119 T18151 T18152 nummod S2,shows
R14120 T18152 T18152 ROOT shows,shows
R14121 T18153 T18155 amod effector,expression
R14122 T18154 T18155 compound protein,expression
R14123 T18155 T18152 dobj expression,shows
R14124 T18156 T18152 prep by,shows
R14125 T18157 T18158 compound Runx3,WT
R14126 T18158 T18156 pobj WT,by
R14127 T18159 T18158 cc and,WT
R14128 T18160 T18158 conj KO,WT
R14129 T18161 T18152 conj cells,shows
R14130 T18162 T18152 prep at,shows
R14131 T18163 T18162 pobj day,at
R14132 T18164 T18163 nummod 4,day
R14133 T18165 T18163 prep of,day
R14134 T18166 T18152 prep in,shows
R14135 T18167 T18168 amod vitro,culture
R14136 T18168 T18166 pobj culture,in
R14137 T18169 T18152 punct .,shows
R14138 T18170 T18171 compound Primer,sequences
R14139 T18171 T18177 nsubjpass sequences,shown
R14140 T18172 T18171 acl used,sequences
R14141 T18173 T18172 prep for,used
R14142 T18174 T18175 compound ChIP,experiments
R14143 T18175 T18173 pobj experiments,for
R14144 T18176 T18177 auxpass are,shown
R14145 T18177 T18177 ROOT shown,shown
R14146 T18178 T18177 prep in,shown
R14147 T18179 T18180 compound Table,S1
R14148 T18180 T18178 pobj S1,in
R14149 T18181 T18177 punct .,shown
R14150 T18182 T18184 amod Online,material
R14151 T18183 T18184 amod supplemental,material
R14152 T18184 T18185 nsubj material,is
R14153 T18185 T18185 ROOT is,is
R14154 T18186 T18185 acomp available,is
R14155 T18187 T18186 prep at,available
R14156 T18188 T18187 pobj http://www.jem.org/cgi/content/full/jem.20081242/DC1,at
R14157 T18189 T18185 punct .,is
R14236 T18364 T18364 ROOT Kinetics,Kinetics
R14237 T18365 T18364 prep of,Kinetics
R14238 T18366 T18367 compound gene,expression
R14239 T18367 T18365 pobj expression,of
R14240 T18368 T18364 prep during,Kinetics
R14241 T18369 T18373 compound CD8,differentiation
R14242 T18370 T18373 compound +,differentiation
R14243 T18371 T18372 compound T,cell
R14244 T18372 T18373 compound cell,differentiation
R14245 T18373 T18368 pobj differentiation,during
R14246 T18374 T18364 punct .,Kinetics
R14247 T18375 T18378 punct (,Kinetics
R14248 T18376 T18378 det A,Kinetics
R14249 T18377 T18378 punct ),Kinetics
R14250 T18378 T18392 nmod Kinetics,expression
R14251 T18379 T18378 prep of,Kinetics
R14252 T18380 T18379 pobj Prf1,of
R14253 T18381 T18380 punct ",",Prf1
R14267 T18395 T18396 compound P14,CD8
R14268 T18396 T18394 dobj CD8,differentiating
R14269 T18397 T18399 amod +,cells
R14270 T18398 T18399 compound T,cells
R14271 T18399 T18394 dobj cells,differentiating
R14272 T18400 T18399 acl analyzed,cells
R14273 T18401 T18400 agent by,analyzed
R14274 T18402 T18403 compound Northern,blotting
R14275 T18403 T18401 pobj blotting,by
R14276 T18404 T18392 punct .,expression
R14277 T18405 T18410 nmod RNA,cells
R14278 T18406 T18405 prep from,RNA
R14279 T18407 T18406 pobj day,from
R14280 T18408 T18410 nummod 7,cells
R14281 T18409 T18410 nummod Th1,cells
R14282 T18410 T18412 nsubjpass cells,used
R14283 T18411 T18412 auxpass was,used
R14284 T18412 T18412 ROOT used,used
R14285 T18413 T18412 prep as,used
R14286 T18414 T18415 det a,control
R14287 T18415 T18413 pobj control,as
R14288 T18416 T18412 punct .,used
R14289 T18417 T18422 nsubjpass Sizes,indicated
R14290 T18418 T18417 prep of,Sizes
R14291 T18419 T18420 compound mRNA,transcripts
R14292 T18420 T18418 pobj transcripts,of
R14293 T18421 T18422 auxpass are,indicated
R14294 T18422 T18422 ROOT indicated,indicated
R14295 T18423 T18422 punct .,indicated
R14296 T18424 T18427 punct (,Quantification
R14297 T18425 T18427 nmod B,Quantification
R14298 T18426 T18427 punct ),Quantification
R14299 T18427 T18427 ROOT Quantification,Quantification
R14300 T18428 T18427 prep of,Quantification
R14351 T18479 T18480 compound granzyme,B
R14352 T18480 T18478 pobj B,for
R14353 T18481 T18480 punct ",",B
R14354 T18482 T18480 appos IFN-γ,B
R14355 T18483 T18477 punct ",",staining
R14356 T18484 T18477 cc and,staining
R14357 T18485 T18477 conj TNF,staining
R14358 T18486 T18476 punct .,Intracellular
R14359 T18487 T18488 compound Granzyme,B
R14360 T18488 T18490 nsubj B,was
R14361 T18489 T18488 acl staining,B
R14362 T18490 T18490 ROOT was,was
R14363 T18491 T18492 amod specific,relative
R14364 T18492 T18490 acomp relative,was
R14365 T18493 T18492 prep to,relative
R14366 T18494 T18496 det an,control
R14367 T18495 T18496 compound isotype,control
R14368 T18496 T18493 pobj control,to
R14369 T18497 T18499 punct (,depicted
R14370 T18498 T18499 neg not,depicted
R14371 T18499 T18496 relcl depicted,control
R14372 T18500 T18490 punct ),was
R14373 T18501 T18490 punct .,was
R14374 T18502 T18504 nsubjpass Cells,restimulated
R14375 T18503 T18504 auxpass were,restimulated
R14376 T18504 T18504 ROOT restimulated,restimulated
R14377 T18505 T18504 prep with,restimulated
R14378 T18506 T18505 pobj PMA,with
R14379 T18507 T18506 cc and,PMA
R14380 T18508 T18506 conj ionomycin,PMA
R14381 T18509 T18504 prep for,restimulated
R14382 T18510 T18511 nummod 4,h.
R14383 T18511 T18509 pobj h.,for
R14384 T18512 T18513 punct (,F
R14385 T18513 T18511 appos F,h.
R14386 T18514 T18504 punct ),restimulated
R14387 T18515 T18516 amod FACS-based,assay
R14388 T18516 T18524 nsubj assay,+
R14389 T18517 T18518 aux to,measure
R14390 T18518 T18516 acl measure,assay
R14391 T18519 T18520 amod cytolytic,activity
R14392 T18520 T18518 dobj activity,measure
R14468 T18596 T18597 compound granule,exocytosis
R14469 T18597 T18594 pobj exocytosis,of
R14470 T18598 T18601 punct (,B
R14471 T18599 T18601 nmod perforin,B
R14472 T18600 T18601 compound granzyme,B
R14473 T18601 T18597 appos B,exocytosis
R14474 T18602 T18601 punct ),B
R14475 T18603 T18597 appos pathway,exocytosis
R14476 T18604 T18580 punct .,blocked
R14477 T18605 T18606 nsubj Data,are
R14478 T18606 T18606 ROOT are,are
R14479 T18607 T18622 nmod representative,experiments
R14480 T18608 T18607 prep of,representative
R14481 T18609 T18610 advmod at,least
R14482 T18610 T18611 advmod least,five
R14483 T18611 T18608 pobj five,of
R14484 T18612 T18614 punct (,E
R14485 T18613 T18614 det A,E
R14486 T18614 T18607 appos E,representative
R14487 T18615 T18614 punct ),E
R14488 T18616 T18607 cc or,representative
R14489 T18617 T18619 nummod three,F
R14490 T18618 T18619 punct (,F
R14491 T18619 T18607 conj F,representative
R14492 T18620 T18619 punct ),F
R14493 T18621 T18622 amod independent,experiments
R14494 T18622 T18606 attr experiments,are
R14495 T18623 T18606 punct .,are
R14953 T19300 T19300 ROOT analysis,analysis
R14954 T19301 T19300 prep of,analysis
R14955 T19302 T19301 pobj Prf1,of
R14956 T19303 T19302 cc and,Prf1
R14957 T19304 T19305 compound GzmB,mRNA
R14958 T19305 T19306 compound mRNA,expression
R14959 T19306 T19300 conj expression,analysis
R14960 T19307 T19300 prep in,analysis
R14961 T19308 T19307 pobj WT,in
R14962 T19309 T19308 cc or,WT
R14963 T19310 T19308 conj T-bet,WT
R14964 T19311 T19315 amod deficient,cells
R14965 T19312 T19315 nmod CD8,cells
R14966 T19313 T19315 nummod +,cells
R14967 T19314 T19315 compound T,cells
R14968 T19315 T19300 appos cells,analysis
R14969 T19316 T19315 acl activated,cells
R14970 T19317 T19316 cc and,activated
R14971 T19318 T19319 preconj either,left
R14972 T19319 T19316 conj left,activated
R14973 T19320 T19319 oprd uninfected,left
R14974 T19321 T19322 punct (,uninf
R14975 T19322 T19320 dep uninf,uninfected
R14976 T19323 T19322 punct ),uninf
R14977 T19324 T19322 cc or,uninf
R14978 T19325 T19322 conj transduced,uninf
R14979 T19326 T19325 prep with,transduced
R14980 T19327 T19328 nsubj retroviruses,expressing
R14981 T19328 T19326 pcomp expressing,with
R14982 T19329 T19328 dobj Eomes-VP16,expressing
R14983 T19330 T19331 punct (,Eo-VP16
R14984 T19331 T19329 appos Eo-VP16,Eomes-VP16
R14985 T19332 T19331 punct ),Eo-VP16
R14301 T18429 T18431 amod relative,amounts
R14393 T18521 T18520 prep of,activity
R14394 T18522 T18523 compound P14,CD8
R14395 T18523 T18521 pobj CD8,of
R14396 T18524 T18524 ROOT +,+
R14397 T18525 T18526 compound T,cells
R14398 T18526 T18524 dobj cells,+
R14399 T18527 T18524 prep against,+
R14400 T18528 T18529 nummod EL4,targets
R14401 T18529 T18527 pobj targets,against
R14402 T18530 T18524 prep loaded,+
R14403 T18531 T18530 prep with,loaded
R14404 T18532 T18534 nummod 0,−
R14405 T18533 T18532 punct (,0
R14406 T18534 T18531 pobj −,with
R14407 T18535 T18530 punct ),loaded
R14408 T18536 T18524 cc or,+
R14409 T18537 T18539 nummod 1,+
R14410 T18538 T18537 punct (,1
R14411 T18539 T18543 nmod +,peptide
R14412 T18540 T18543 punct ),peptide
R14413 T18541 T18543 compound μM,peptide
R14414 T18542 T18543 compound Gp33,peptide
R14415 T18543 T18546 nmod peptide,ratio
R14416 T18544 T18546 punct (,ratio
R14417 T18545 T18546 amod effector-to-target,ratio
R14418 T18546 T18548 nsubj ratio,5:1
R14419 T18547 T18548 punct =,5:1
R14420 T18548 T18524 conj 5:1,+
R14421 T18549 T18548 punct ),5:1
R14422 T18550 T18524 punct .,+
R14423 T18551 T18572 nsubjpass Percentage,determined
R14424 T18552 T18551 prep of,Percentage
R14425 T18553 T18555 compound Annexin,+
R14426 T18554 T18555 compound V,+
R14427 T18555 T18552 pobj +,of
R14428 T18556 T18557 punct (,apoptotic
R14429 T18557 T18560 amod apoptotic,cells
R14430 T18558 T18557 punct ),apoptotic
R14431 T18559 T18560 compound target,cells
R14432 T18560 T18551 conj cells,Percentage
R14433 T18561 T18560 prep in,cells
R14434 T18562 T18566 det the,population
R14435 T18563 T18566 amod CD8-negative,population
R14436 T18564 T18566 nummod EL4,population
R14437 T18565 T18566 compound target,population
R14438 T18566 T18561 pobj population,in
R14439 T18567 T18568 punct (,dot
R14440 T18568 T18569 amod dot,plots
R14441 T18569 T18560 conj plots,cells
R14442 T18570 T18560 punct ),cells
R14443 T18571 T18572 auxpass was,determined
R14444 T18572 T18572 ROOT determined,determined
R14445 T18573 T18572 punct (,determined
R14446 T18574 T18572 oprd histograms,determined
R14447 T18575 T18572 punct ),determined
R14448 T18576 T18572 punct .,determined
R14449 T18577 T18578 amod Cytolytic,activity
R14450 T18578 T18580 nsubjpass activity,blocked
R14451 T18579 T18580 auxpass was,blocked
R14452 T18580 T18580 ROOT blocked,blocked
R14453 T18581 T18580 agent by,blocked
R14454 T18582 T18581 pobj incubation,by
R14455 T18583 T18580 prep with,blocked
R14456 T18584 T18586 nummod 2,EGTA
R14457 T18585 T18586 compound mM,EGTA
R14458 T18586 T18583 pobj EGTA,with
R14459 T18587 T18589 punct (,depicted
R14460 T18588 T18589 neg not,depicted
R14461 T18589 T18586 parataxis depicted,EGTA
R14462 T18590 T18589 punct ),depicted
R14463 T18591 T18580 punct ",",blocked
R14464 T18592 T18580 advcl confirming,blocked
R14465 T18593 T18592 dobj involvement,confirming
R14466 T18594 T18593 prep of,involvement
R14467 T18595 T18597 det the,exocytosis
R14860 T19207 T19207 ROOT Regulation,Regulation
R14861 T19208 T19207 prep of,Regulation
R14862 T19209 T19208 pobj perforin,of
R14863 T19210 T19207 punct ",",Regulation
R14864 T19211 T19212 compound granzyme,B
R14865 T19212 T19207 appos B,Regulation
R14866 T19213 T19207 punct ",",Regulation
R14867 T19214 T19207 cc and,Regulation
R14868 T19215 T19216 compound IFN-γ,expression
R14869 T19216 T19207 conj expression,Regulation
R14870 T19217 T19207 prep by,Regulation
R14871 T19218 T19217 pobj T-bet,by
R14872 T19219 T19218 cc and,T-bet
R14873 T19220 T19218 conj Eomes,T-bet
R14874 T19221 T19207 prep in,Regulation
R14875 T19222 T19221 pcomp differentiating,in
R14876 T19223 T19222 dobj CTLs,differentiating
R14877 T19224 T19207 punct .,Regulation
R14878 T19225 T19226 punct (,A
R14879 T19226 T19229 nmod A,expression
R14880 T19227 T19226 punct ),A
R14881 T19228 T19229 amod IFN-γ,expression
R14882 T19229 T19229 ROOT expression,expression
R14883 T19230 T19229 prep by,expression
R14884 T19231 T19230 pobj WT,by
R14885 T19232 T19236 punct (,+
R14886 T19233 T19236 nmod Tbx21,+
R14887 T19234 T19235 compound +,/
R14888 T19235 T19233 nummod /,Tbx21
R14889 T19236 T19231 appos +,WT
R14890 T19237 T19236 punct ),+
R14891 T19238 T19231 cc and,WT
R14892 T19239 T19231 conj T-bet,WT
R14893 T19240 T19229 appos deficient,expression
R14894 T19241 T19240 punct (,deficient
R14895 T19242 T19243 nummod Tbx21,−
R14896 T19243 T19245 nummod −,−
R14897 T19244 T19245 compound /,−
R14898 T19245 T19240 appos −,deficient
R14899 T19246 T19240 punct ),deficient
R14900 T19247 T19248 compound T,cells
R14901 T19248 T19229 appos cells,expression
R14902 T19249 T19229 punct .,expression
R14903 T19250 T19254 amod Naive,cells
R14904 T19251 T19254 nmod CD8,cells
R14905 T19252 T19254 nummod +,cells
R14906 T19253 T19254 compound T,cells
R14907 T19254 T19268 nsubjpass cells,restimulated
R14908 T19255 T19254 punct ",",cells
R14909 T19256 T19254 cc or,cells
R14910 T19257 T19254 conj cells,cells
R14911 T19258 T19257 acl activated,cells
R14912 T19259 T19258 cc and,activated
R14913 T19260 T19258 conj cultured,activated
R14914 T19261 T19260 prep for,cultured
R14915 T19262 T19265 nummod 4,d
R14916 T19263 T19262 cc or,4
R14917 T19264 T19262 conj 6,4
R14918 T19265 T19261 pobj d,for
R14919 T19266 T19268 punct ",",restimulated
R14920 T19267 T19268 auxpass were,restimulated
R14921 T19268 T19268 ROOT restimulated,restimulated
R14923 T19270 T19269 pobj PMA,with
R14924 T19271 T19270 cc and,PMA
R14925 T19272 T19270 conj ionomycin,PMA
R14926 T19273 T19268 prep for,restimulated
R14927 T19274 T19275 nummod 6,h
R14928 T19275 T19273 pobj h,for
R14929 T19276 T19268 punct ",",restimulated
R14930 T19277 T19268 cc and,restimulated
R14931 T19278 T19279 compound IFN-γ,expression
R14932 T19279 T19281 nsubjpass expression,assessed
R14933 T19280 T19281 auxpass was,assessed
R14934 T19281 T19268 conj assessed,restimulated
R14935 T19282 T19281 agent by,assessed
R14936 T19283 T19284 amod intracellular,staining
R14937 T19284 T19282 pobj staining,by
R14938 T19285 T19281 punct .,assessed
R14939 T19286 T19287 nsubj Numbers,show
R14940 T19287 T19287 ROOT show,show
R14941 T19288 T19289 det the,percentage
R14942 T19289 T19287 dobj percentage,show
R14986 T19333 T19329 cc or,Eomes-VP16
R14987 T19334 T19337 det an,cassette
R14988 T19335 T19337 amod empty,cassette
R14989 T19336 T19337 compound IRES-GFP,cassette
R14990 T19337 T19329 conj cassette,Eomes-VP16
R14991 T19338 T19339 punct (,GFP
R14992 T19339 T19337 appos GFP,cassette
R14993 T19340 T19300 punct ),analysis
R14994 T19341 T19300 punct .,analysis
R14995 T19342 T19344 amod Total,RNA
R14996 T19343 T19344 amod cellular,RNA
R14997 T19344 T19346 nsubjpass RNA,analyzed
R14998 T19345 T19346 auxpass was,analyzed
R14999 T19346 T19346 ROOT analyzed,analyzed
R15000 T19347 T19346 prep on,analyzed
R15001 T19348 T19347 pobj day,on
R15002 T19349 T19348 nummod 6,day
R15003 T19350 T19348 prep of,day
R15004 T19351 T19350 pobj culture,of
R15005 T19352 T19346 punct .,analyzed
R15006 T19353 T19354 det The,frequency
R15007 T19354 T19361 nsubj frequency,was
R15008 T19355 T19354 prep of,frequency
R15009 T19356 T19357 amod transduced,cells
R15010 T19357 T19355 pobj cells,of
R15011 T19358 T19354 prep in,frequency
R15012 T19359 T19360 det the,cultures
R15013 T19360 T19358 pobj cultures,in
R15014 T19361 T19361 ROOT was,was
R15015 T19362 T19361 acomp equivalent,was
R15016 T19363 T19362 prep for,equivalent
R15017 T19364 T19365 det both,constructs
R15018 T19365 T19363 pobj constructs,for
R15019 T19366 T19376 punct (,depicted
R15020 T19367 T19368 nummod ∼,65
R15021 T19368 T19369 quantmod 65,70
R15022 T19369 T19370 nummod 70,%
R15023 T19370 T19376 nsubj %,depicted
R15024 T19371 T19373 nmod GFP,cells
R15025 T19372 T19373 nummod +,cells
R15026 T19373 T19370 appos cells,%
R15027 T19374 T19370 punct ;,%
R15103 T19450 T19452 amod IFN-γ,cells
R15104 T19451 T19452 compound +,cells
R15105 T19452 T19446 conj cells,percentage
R15106 T19453 T19444 punct .,show
R15107 T19454 T19455 nsubj Results,are
R15108 T19455 T19455 ROOT are,are
R15109 T19456 T19455 attr representative,are
R15110 T19457 T19456 prep of,representative
R15111 T19458 T19472 nummod three,experiments
R15112 T19459 T19460 punct (,A
R15113 T19460 T19458 appos A,three
R15114 T19461 T19460 cc and,A
R15115 T19462 T19460 conj C,A
R15116 T19463 T19462 punct ),C
R15117 T19464 T19465 cc or,two
R15118 T19465 T19460 conj two,A
R15119 T19466 T19467 punct (,B
R15120 T19467 T19472 nmod B,experiments
R15121 T19468 T19467 cc and,B
R15122 T19469 T19467 conj D,B
R15123 T19470 T19467 punct ),B
R15124 T19471 T19472 amod independent,experiments
R15125 T19472 T19457 pobj experiments,of
R15126 T19473 T19455 punct .,are
R15583 T20215 T20223 nmod Runx3,cells
R15584 T20216 T20223 nmod −,cells
R15585 T20217 T20223 nmod /,cells
R15586 T20218 T20223 nmod −,cells
R15587 T20219 T20223 nmod CD8,cells
R15588 T20220 T20223 nmod +,cells
R15589 T20221 T20223 nmod SP,cells
R15590 T20222 T20223 compound T,cells
R15591 T20223 T20224 nsubj cells,differentiated
R15592 T20224 T20212 ccomp differentiated,/
R15593 T20225 T20224 prep for,differentiated
R15594 T20226 T20228 nummod 6,β-Actin
R15595 T20227 T20228 compound d.,β-Actin
R15596 T20228 T20225 pobj β-Actin,for
R15597 T20229 T20230 auxpass was,used
R15598 T20230 T20212 conj used,/
R15599 T20231 T20230 prep as,used
R15600 T20232 T20234 det a,control
R15601 T20233 T20234 compound loading,control
R15602 T20234 T20231 pobj control,as
R15603 T20235 T20212 punct .,/
R15604 T20236 T20241 punct (,analysis
R15605 T20237 T20241 nmod B,analysis
R15606 T20238 T20237 punct ),B
R15607 T20239 T20240 compound Northern,blot
R15608 T20240 T20241 compound blot,analysis
R15609 T20241 T20249 nsubj analysis,/
R15610 T20242 T20241 prep of,analysis
R15611 T20243 T20244 compound Prf1,mRNA
R15612 T20244 T20245 compound mRNA,expression
R15613 T20245 T20242 pobj expression,of
R15614 T20246 T20241 prep in,analysis
R15615 T20247 T20248 compound Runx3,+
R15616 T20248 T20246 pobj +,in
R15617 T20249 T20267 ccomp /,used
R15618 T20250 T20259 nummod +,cells
R15619 T20251 T20250 cc versus,+
R15620 T20252 T20259 nmod Runx3,cells
R15621 T20253 T20259 nmod −,cells
R15622 T20254 T20259 nmod /,cells
R15623 T20255 T20259 nmod −,cells
R15624 T20256 T20259 nmod CD8,cells
R15625 T20257 T20259 nmod +,cells
R14943 T19290 T19289 prep of,percentage
R14944 T19291 T19293 amod IFN-γ,cells
R14945 T19292 T19293 compound +,cells
R14946 T19293 T19290 pobj cells,of
R14947 T19294 T19287 punct .,show
R14948 T19295 T19300 punct (,analysis
R14949 T19296 T19300 nmod B,analysis
R14950 T19297 T19296 punct ),B
R14951 T19298 T19299 compound Northern,blot
R14952 T19299 T19300 compound blot,analysis
R15028 T19375 T19376 neg not,depicted
R15029 T19376 T19361 parataxis depicted,was
R15030 T19377 T19376 punct ),depicted
R15031 T19378 T19361 punct .,was
R15032 T19379 T19386 punct (,expression
R15033 T19380 T19386 nmod C,expression
R15034 T19381 T19380 punct ),C
R15035 T19382 T19383 compound Granzyme,B
R15036 T19383 T19380 appos B,C
R15037 T19384 T19383 cc and,B
R15038 T19385 T19386 compound IFN-γ,expression
R15039 T19386 T19414 nmod expression,production
R15040 T19387 T19386 prep by,expression
R15041 T19388 T19387 pobj Tbx21,by
R15042 T19389 T19390 compound +,/
R15043 T19390 T19390 ROOT /,/
R15044 T19391 T19395 nummod +,/
R15045 T19392 T19391 cc and,+
R15046 T19393 T19394 nummod Tbx21,−
R15047 T19394 T19391 conj −,+
R15048 T19395 T19390 dobj /,/
R15049 T19396 T19398 nummod −,cells
R15050 T19397 T19398 compound T,cells
R15051 T19398 T19395 appos cells,/
R15052 T19399 T19398 acl analyzed,cells
R15053 T19400 T19399 prep in,analyzed
R15054 T19401 T19402 amod restimulated,cells
R15055 T19402 T19400 pobj cells,in
R15056 T19403 T19406 nsubjpass that,cultured
R15057 T19404 T19406 aux had,cultured
R15058 T19405 T19406 auxpass been,cultured
R15059 T19406 T19402 relcl cultured,cells
R15060 T19407 T19406 prep for,cultured
R15061 T19408 T19409 nummod 5,d.
R15062 T19409 T19407 pobj d.,for
R15063 T19410 T19411 punct (,D
R15064 T19411 T19409 appos D,d.
R15065 T19412 T19390 punct ),/
R15066 T19413 T19414 amod IFN-γ,production
R15067 T19414 T19414 ROOT production,production
R15068 T19415 T19414 prep by,production
R15069 T19416 T19415 pobj cells,by
R15070 T19417 T19416 acl transduced,cells
R15071 T19418 T19417 prep with,transduced
R15072 T19419 T19418 pobj Eo-VP16,with
R15073 T19420 T19419 cc or,Eo-VP16
R15074 T19421 T19419 conj control,Eo-VP16
R15075 T19422 T19419 punct (,Eo-VP16
R15076 T19423 T19419 appos GFP,Eo-VP16
R15077 T19424 T19419 punct ),Eo-VP16
R15078 T19425 T19419 appos retroviruses,Eo-VP16
R15079 T19426 T19425 punct (,retroviruses
R15080 T19427 T19425 appos RV,retroviruses
R15081 T19428 T19425 punct ),retroviruses
R15082 T19429 T19414 acl measured,production
R15083 T19430 T19429 prep on,measured
R15084 T19431 T19430 pobj day,on
R15085 T19432 T19431 nummod 4,day
R15086 T19433 T19431 prep after,day
R15087 T19434 T19435 nummod 6,h
R15088 T19435 T19433 pobj h,after
R15089 T19436 T19435 prep of,h
R15090 T19437 T19436 pobj restimulation,of
R15091 T19438 T19435 prep with,h
R15092 T19439 T19438 pobj PMA,with
R15093 T19440 T19439 cc and,PMA
R15094 T19441 T19439 conj ionomycin,PMA
R15095 T19442 T19414 punct .,production
R15096 T19443 T19444 nsubj Numbers,show
R15097 T19444 T19444 ROOT show,show
R15098 T19445 T19446 det the,percentage
R15099 T19446 T19444 dobj percentage,show
R15100 T19447 T19446 prep of,percentage
R15101 T19448 T19447 pobj GFP,of
R15102 T19449 T19452 amod +,cells
R15553 T20185 T20186 amod Key,role
R15554 T20186 T20186 ROOT role,role
R15555 T20187 T20186 prep for,role
R15556 T20188 T20187 pobj Runx3,for
R15557 T20189 T20186 prep in,role
R15558 T20190 T20191 compound effector,CTL
R15559 T20191 T20192 compound CTL,differentiation
R15560 T20192 T20189 pobj differentiation,in
R15561 T20193 T20186 punct .,role
R15562 T20194 T20198 punct (,analysis
R15563 T20195 T20198 nmod A,analysis
R15564 T20196 T20195 punct ),A
R15565 T20197 T20198 amod Western,analysis
R15566 T20198 T20212 nsubj analysis,/
R15567 T20199 T20198 prep of,analysis
R15568 T20200 T20199 pobj Runx3,of
R15569 T20201 T20200 punct ",",Runx3
R15570 T20202 T20200 conj Eomes,Runx3
R15571 T20203 T20202 punct ",",Eomes
R15572 T20204 T20202 conj T-bet,Eomes
R15573 T20205 T20204 punct ",",T-bet
R15574 T20206 T20204 cc and,T-bet
R15575 T20207 T20208 compound perforin,expression
R15576 T20208 T20198 conj expression,analysis
R15577 T20209 T20198 prep in,analysis
R15578 T20210 T20211 compound Runx3,+
R15579 T20211 T20209 pobj +,in
R15580 T20212 T20212 ROOT /,/
R15581 T20213 T20223 nummod +,cells
R15582 T20214 T20213 cc versus,+
R15658 T20290 T20288 conj restimulated,resting
R15659 T20291 T20290 punct (,restimulated
R15660 T20292 T20293 nummod 6,h
R15661 T20293 T20290 appos h,restimulated
R15662 T20294 T20290 punct ),restimulated
R15663 T20295 T20298 amod Runx3,+
R15664 T20296 T20298 compound +,+
R15665 T20297 T20298 compound /,+
R15666 T20298 T20298 ROOT +,+
R15667 T20299 T20302 prep versus,/
R15668 T20300 T20301 compound Runx3,−
R15669 T20301 T20302 nsubj −,/
R15670 T20302 T20302 ROOT /,/
R15671 T20303 T20308 nummod −,cells
R15672 T20304 T20308 nummod CD8,cells
R15673 T20305 T20308 compound +,cells
R15674 T20306 T20307 compound SP,T
R15675 T20307 T20308 compound T,cells
R15676 T20308 T20309 nsubj cells,differentiated
R15677 T20309 T20302 acl differentiated,/
R15678 T20310 T20309 prep for,differentiated
R15679 T20311 T20312 nummod 6,d
R15680 T20312 T20310 pobj d,for
R15681 T20313 T20309 punct .,differentiated
R15682 T20314 T20317 det The,line
R15683 T20315 T20317 amod vertical,line
R15684 T20316 T20317 amod gray,line
R15685 T20317 T20318 nsubj line,indicates
R15686 T20318 T20318 ROOT indicates,indicates
R15687 T20319 T20322 det the,MFI
R15688 T20320 T20322 compound granzyme,MFI
R15689 T20321 T20322 compound B,MFI
R15690 T20322 T20318 dobj MFI,indicates
R15691 T20323 T20322 prep for,MFI
R15692 T20324 T20325 compound WT,GFP
R15693 T20325 T20327 compound GFP,cells
R15694 T20326 T20327 compound +,cells
R15695 T20327 T20323 pobj cells,for
R15696 T20328 T20318 punct .,indicates
R15697 T20329 T20333 nsubj Results,are
R15698 T20330 T20329 prep in,Results
R15699 T20331 T20330 pobj A,in
R15700 T20332 T20333 nsubj C,are
R15626 T20258 T20259 compound T,cells
R15627 T20259 T20260 nsubj cells,differentiated
R15628 T20260 T20249 ccomp differentiated,/
R15629 T20261 T20260 prep for,differentiated
R15630 T20262 T20264 nummod 6,β
R15631 T20263 T20264 compound d.,β
R15632 T20264 T20261 pobj β,for
R15633 T20265 T20267 nsubjpass Actin,used
R15634 T20266 T20267 auxpass was,used
R15635 T20267 T20267 ROOT used,used
R15636 T20268 T20267 prep as,used
R15637 T20269 T20271 det a,control
R15638 T20270 T20271 compound loading,control
R15639 T20271 T20268 pobj control,as
R15640 T20272 T20267 punct .,used
R15641 T20273 T20274 punct (,C
R15642 T20274 T20276 compound C,Expression
R15643 T20275 T20274 punct ),C
R15644 T20276 T20276 ROOT Expression,Expression
R15645 T20277 T20276 prep of,Expression
R15646 T20278 T20279 compound granzyme,B
R15647 T20279 T20277 pobj B,of
R15648 T20280 T20279 punct ",",B
R15649 T20281 T20279 appos IFN-γ,B
R15650 T20282 T20281 punct ",",IFN-γ
R15651 T20283 T20281 conj TNF,IFN-γ
R15652 T20284 T20283 punct ",",TNF
R15653 T20285 T20283 cc and,TNF
R15654 T20286 T20283 conj IL-2,TNF
R15655 T20287 T20276 prep by,Expression
R15656 T20288 T20287 pcomp resting,by
R15657 T20289 T20288 cc or,resting
R16018 T20967 T20968 amod Runx3,controls
R16019 T20968 T20968 ROOT controls,controls
R16020 T20969 T20968 appos Eomes,controls
R16021 T20970 T20969 punct ",",Eomes
R16022 T20971 T20969 appos perforin,Eomes
R16023 T20972 T20969 punct ",",Eomes
R16024 T20973 T20974 compound granzyme,B
R16025 T20974 T20969 conj B,Eomes
R16026 T20975 T20969 punct ",",Eomes
R16027 T20976 T20969 cc and,Eomes
R16028 T20977 T20978 compound IFN-γ,expression
R16029 T20978 T20969 conj expression,Eomes
R16030 T20979 T20978 prep in,expression
R16031 T20980 T20981 compound effector,CTLs
R16032 T20981 T20979 pobj CTLs,in
R16033 T20982 T20968 punct .,controls
R16034 T20983 T20986 amod Runx3,+
R16035 T20984 T20986 compound +,+
R16036 T20985 T20986 compound /,+
R16037 T20986 T20997 nsubjpass +,activated
R16038 T20987 T20986 cc or,+
R16039 T20988 T20995 amod Runx3,cells
R16040 T20989 T20995 nmod −,cells
R16041 T20990 T20995 nmod /,cells
R16042 T20991 T20995 nmod −,cells
R16043 T20992 T20995 nmod CD8,cells
R16044 T20993 T20995 nmod +,cells
R16045 T20994 T20995 compound T,cells
R16046 T20995 T20986 conj cells,+
R16047 T20996 T20997 auxpass were,activated
R16048 T20997 T20997 ROOT activated,activated
R16049 T20998 T20997 cc and,activated
R16050 T20999 T20997 conj transduced,activated
R16051 T21000 T20999 prep with,transduced
R16052 T21001 T21000 pobj retroviruses,with
R16053 T21002 T21001 acl bearing,retroviruses
R16054 T21003 T21006 det an,cassette
R16055 T21004 T21006 amod empty,cassette
R16056 T21005 T21006 compound IRES-GFP,cassette
R16057 T21006 T21002 dobj cassette,bearing
R16058 T21007 T21006 punct (,cassette
R16059 T21008 T21006 appos GFP,cassette
R16060 T21009 T21008 punct ),GFP
R16061 T21010 T21006 cc or,cassette
R16062 T21011 T21012 advmod also,encoding
R16063 T21012 T20997 advcl encoding,activated
R16064 T21013 T21012 dobj Eomes-VP16,encoding
R16065 T21014 T21015 punct (,Eo-VP16
R16066 T21015 T21013 appos Eo-VP16,Eomes-VP16
R16067 T21016 T21015 punct ),Eo-VP16
R16068 T21017 T21013 cc or,Eomes-VP16
R16069 T21018 T21013 conj Myc-Runx3,Eomes-VP16
R16070 T21019 T21020 punct (,Runx3
R16071 T21020 T21018 appos Runx3,Myc-Runx3
R16072 T21021 T21020 punct ),Runx3
R16073 T21022 T20997 punct .,activated
R16074 T21023 T21024 det The,frequency
R16075 T21024 T21031 nsubj frequency,was
R16076 T21025 T21024 prep of,frequency
R16077 T21026 T21027 amod transduced,cells
R16078 T21027 T21025 pobj cells,of
R16079 T21028 T21024 prep in,frequency
R16080 T21029 T21030 det the,cultures
R16081 T21030 T21028 pobj cultures,in
R16082 T21031 T21031 ROOT was,was
R16083 T21032 T21031 acomp equivalent,was
R16084 T21033 T21032 prep for,equivalent
R16085 T21034 T21035 det all,constructs
R16086 T21035 T21033 pobj constructs,for
R16087 T21036 T21035 punct (,constructs
R16088 T21037 T21038 nmod ∼,75
R16089 T21038 T21035 appos 75,constructs
R16090 T21039 T21040 nummod 90,%
R16091 T21040 T21043 compound %,cells
R16092 T21041 T21043 compound GFP,cells
R16093 T21042 T21043 nummod +,cells
R16094 T21043 T21035 appos cells,constructs
R16170 T21119 T21120 auxpass was,determined
R16171 T21120 T21120 ROOT determined,determined
R16172 T21121 T21120 agent by,determined
R16173 T21122 T21123 amod intracellular,staining
R16174 T21123 T21121 pobj staining,by
R16175 T21124 T21120 punct .,determined
R16176 T21125 T21126 det The,percentage
R15701 T20333 T20333 ROOT are,are
R15702 T20334 T20333 attr representative,are
R15703 T20335 T20334 prep of,representative
R15704 T20336 T20338 nummod two,experiments
R15705 T20337 T20338 amod independent,experiments
R15706 T20338 T20335 pobj experiments,of
R15707 T20339 T20333 punct .,are
R15708 T20340 T20344 punct (,analysis
R15709 T20341 T20344 compound D,analysis
R15710 T20342 T20343 punct ),ChIP
R15711 T20343 T20344 compound ChIP,analysis
R15712 T20344 T20344 ROOT analysis,analysis
R15713 T20345 T20344 prep of,analysis
R15714 T20346 T20345 pcomp binding,of
R15715 T20347 T20346 prep of,binding
R15716 T20348 T20349 compound endogenous,Runx3
R15717 T20349 T20347 pobj Runx3,of
R15718 T20350 T20349 cc and,Runx3
R15719 T20351 T20349 conj Eomes,Runx3
R15720 T20352 T20344 prep to,analysis
R15721 T20353 T20355 det the,locus
R15722 T20354 T20355 amod Prf1,locus
R15723 T20355 T20352 pobj locus,to
R15724 T20356 T20344 punct .,analysis
R15725 T20357 T20364 nsubjpass Enrichment,evaluated
R15726 T20358 T20357 prep of,Enrichment
R15727 T20359 T20362 det the,regions
R15728 T20360 T20362 amod indicated,regions
R15729 T20361 T20362 amod genomic,regions
R15730 T20362 T20358 pobj regions,of
R15731 T20363 T20364 auxpass was,evaluated
R15732 T20364 T20364 ROOT evaluated,evaluated
R15733 T20365 T20364 agent by,evaluated
R15734 T20366 T20367 amod real-time,PCR
R15735 T20367 T20365 pobj PCR,by
R15736 T20368 T20367 prep of,PCR
R15737 T20369 T20368 pobj DNA,of
R15738 T20370 T20364 prep from,evaluated
R15739 T20371 T20370 pobj immunoprecipitated,from
R15740 T20372 T20371 cc and,immunoprecipitated
R15741 T20373 T20374 compound input,chromatin
R15742 T20374 T20371 conj chromatin,immunoprecipitated
R15743 T20375 T20364 punct .,evaluated
R15744 T20376 T20377 det The,data
R15745 T20377 T20378 nsubj data,are
R15746 T20378 T20378 ROOT are,are
R15747 T20379 T20380 det the,means
R15748 T20380 T20378 attr means,are
R15749 T20381 T20380 prep of,means
R15750 T20382 T20381 pcomp duplicate,of
R15751 T20383 T20382 dobj measurements,duplicate
R15752 T20384 T20382 prep from,duplicate
R15753 T20385 T20387 nummod two,preparations
R15754 T20386 T20387 amod chromatin,preparations
R15755 T20387 T20384 pobj preparations,from
R15756 T20388 T20387 prep from,preparations
R15757 T20389 T20395 nummod two,differentiations
R15758 T20390 T20395 amod independent,differentiations
R15759 T20391 T20395 compound CD8,differentiations
R15760 T20392 T20395 nummod +,differentiations
R15761 T20393 T20394 compound T,cell
R15762 T20394 T20395 compound cell,differentiations
R15763 T20395 T20388 pobj differentiations,from
R15764 T20396 T20378 punct .,are
R15765 T20397 T20398 det The,efficiency
R15766 T20398 T20410 nsubj efficiency,was
R15767 T20399 T20398 prep of,efficiency
R15768 T20400 T20399 pobj recovery,of
R15769 T20401 T20400 prep of,recovery
R15770 T20402 T20401 pobj input,of
R15771 T20403 T20402 prep for,input
R15772 T20404 T20407 det the,region
R15773 T20405 T20407 amod −,region
R15774 T20406 T20407 amod 1-kb,region
R15775 T20407 T20403 pobj region,for
R15776 T20408 T20407 prep of,region
R15777 T20409 T20408 pobj Prf1,of
R15778 T20410 T20410 ROOT was,was
R15779 T20411 T20412 nummod 0.97,%
R15780 T20412 T20410 attr %,was
R15781 T20413 T20410 prep for,was
R15782 T20414 T20416 det the,ChIP
R15783 T20415 T20416 compound Runx3,ChIP
R15784 T20416 T20413 pobj ChIP,for
R15785 T20417 T20410 cc and,was
R15786 T20418 T20419 nummod 0.5,%
R15787 T20419 T20410 conj %,was
R15788 T20420 T20419 prep for,%
R15789 T20421 T20423 det the,ChIP
R15790 T20422 T20423 compound Eomes,ChIP
R15791 T20423 T20420 pobj ChIP,for
R15792 T20424 T20410 punct .,was
R16095 T21044 T21046 punct ;,depicted
R16096 T21045 T21046 neg not,depicted
R16097 T21046 T21031 conj depicted,was
R16098 T21047 T21046 punct ),depicted
R16099 T21048 T21031 punct .,was
R16100 T21049 T21062 punct (,analyzed
R16101 T21050 T21053 nmod A,expression
R16102 T21051 T21050 punct ),A
R16103 T21052 T21053 compound Protein,expression
R16104 T21053 T21062 nsubjpass expression,analyzed
R16105 T21054 T21053 prep in,expression
R16106 T21055 T21056 amod whole-cell,extracts
R16107 T21056 T21054 pobj extracts,in
R16108 T21057 T21058 punct (,day
R16109 T21058 T21053 appos day,expression
R16110 T21059 T21058 nummod 6,day
R16111 T21060 T21053 punct ),expression
R16112 T21061 T21062 auxpass was,analyzed
R16113 T21062 T21062 ROOT analyzed,analyzed
R16114 T21063 T21062 agent by,analyzed
R16115 T21064 T21063 pcomp immunoblotting,by
R16116 T21065 T21062 punct .,analyzed
R16117 T21066 T21072 nsubjpass Overexpression,detected
R16118 T21067 T21066 prep of,Overexpression
R16119 T21068 T21067 pobj Eomes-VP16,of
R16120 T21069 T21072 aux can,detected
R16121 T21070 T21072 neg not,detected
R16122 T21071 T21072 auxpass be,detected
R16123 T21072 T21072 ROOT detected,detected
R16124 T21073 T21072 prep with,detected
R16125 T21074 T21076 det the,antibody
R16126 T21075 T21076 compound Eomes,antibody
R16127 T21076 T21073 pobj antibody,with
R16128 T21077 T21072 punct ",",detected
R16129 T21078 T21082 mark as,is
R16130 T21079 T21081 det the,epitope
R16131 T21080 T21081 amod C-terminal,epitope
R16132 T21081 T21082 nsubj epitope,is
R16133 T21082 T21072 advcl is,detected
R16134 T21083 T21082 prep within,is
R16135 T21084 T21085 det the,region
R16136 T21085 T21083 pobj region,within
R16137 T21086 T21089 nsubjpass that,replaced
R16138 T21087 T21089 aux has,replaced
R16139 T21088 T21089 auxpass been,replaced
R16140 T21089 T21085 relcl replaced,region
R16141 T21090 T21089 prep with,replaced
R16142 T21091 T21094 det the,domain
R16143 T21092 T21094 compound VP16,domain
R16144 T21093 T21094 compound transactivation,domain
R16145 T21094 T21090 pobj domain,with
R16146 T21095 T21072 punct .,detected
R16147 T21096 T21099 punct (,Expression
R16148 T21097 T21099 nmod B,Expression
R16149 T21098 T21097 punct ),B
R16150 T21099 T21099 ROOT Expression,Expression
R16151 T21100 T21099 prep of,Expression
R16152 T21101 T21102 compound granzyme,B
R16153 T21102 T21100 pobj B,of
R16154 T21103 T21102 cc and,B
R16155 T21104 T21102 conj IFN-γ,B
R16156 T21105 T21099 prep after,Expression
R16157 T21106 T21105 pobj culture,after
R16158 T21107 T21099 prep for,Expression
R16159 T21108 T21107 pobj 6,for
R16160 T21109 T21120 nsubjpass d,determined
R16177 T21126 T21132 nsubjpass percentage,shown
R16178 T21127 T21126 prep of,percentage
R16179 T21128 T21129 advmod positively,stained
R16180 T21129 T21130 amod stained,cells
R16181 T21130 T21127 pobj cells,of
R16182 T21131 T21132 auxpass is,shown
R16183 T21132 T21153 ccomp shown,shown
R16184 T21133 T21132 prep above,shown
R16185 T21134 T21135 det the,gate
R16186 T21135 T21133 pobj gate,above
R16187 T21136 T21153 punct ;,shown
R16188 T21137 T21140 det the,intensity
R16189 T21138 T21140 amod mean,intensity
R16190 T21139 T21140 compound fluorescence,intensity
R16191 T21140 T21153 nsubjpass intensity,shown
R16192 T21141 T21142 punct (,MFI
R16193 T21142 T21140 appos MFI,intensity
R16194 T21143 T21142 punct ),MFI
R16195 T21144 T21142 prep of,MFI
R16196 T21145 T21146 compound granzyme,B
R16197 T21146 T21144 pobj B,of
R16198 T21147 T21140 conj staining,intensity
R16199 T21148 T21147 prep for,staining
R16200 T21149 T21151 det the,population
R16201 T21150 T21151 amod total,population
R16202 T21151 T21148 pobj population,for
R16203 T21152 T21153 auxpass is,shown
R16204 T21153 T21153 ROOT shown,shown
R16205 T21154 T21153 prep below,shown
R16206 T21155 T21156 det the,gate
R16207 T21156 T21154 pobj gate,below
R16208 T21157 T21153 punct .,shown
R16209 T21158 T21161 det The,lines
R16210 T21159 T21161 amod vertical,lines
R16211 T21160 T21161 amod gray,lines
R16212 T21161 T21162 nsubj lines,indicate
R16213 T21162 T21162 ROOT indicate,indicate
R16214 T21163 T21164 det the,MFI
R16215 T21164 T21162 dobj MFI,indicate
R16216 T21165 T21162 prep for,indicate
R16217 T21166 T21167 compound WT,GFP
R16218 T21167 T21169 compound GFP,cells
R16219 T21168 T21169 compound +,cells
R16220 T21169 T21165 pobj cells,for
R16221 T21170 T21162 punct .,indicate
R16222 T21171 T21172 nsubj Results,are
R16223 T21172 T21172 ROOT are,are
R16224 T21173 T21172 attr representative,are
R16225 T21174 T21173 prep of,representative
R16226 T21175 T21176 advmod at,least
R16227 T21176 T21177 advmod least,two
R16228 T21177 T21179 nummod two,experiments
R16229 T21178 T21179 amod independent,experiments
R16230 T21179 T21174 pobj experiments,of
R16231 T21180 T21172 punct .,are
R16232 T21181 T21182 punct (,C
R16233 T21182 T21185 nmod C,diagram
R16234 T21183 T21182 punct ),C
R16235 T21184 T21185 amod Schematic,diagram
R16236 T21185 T21185 ROOT diagram,diagram
R16237 T21186 T21185 prep of,diagram
R16238 T21187 T21189 det the,network
R16239 T21188 T21189 amod transcriptional,network
R16240 T21189 T21186 pobj network,of
R16241 T21190 T21189 acl involving,network
R16242 T21191 T21190 dobj Runx3,involving
R16243 T21192 T21191 cc and,Runx3
R16244 T21193 T21191 conj T-box,Runx3
R13741 T17728 T17730 compound pH,]
R13743 T17730 T17722 appos ],buffer
R13745 T17732 T17734 nummod 150,NaCl
R13747 T17734 T17730 appos NaCl,]
R13763 T17750 T17749 dobj samples,resuspending
R13765 T17752 T17753 nummod 10,μl
R13767 T17754 T17753 prep per,μl
R13769 T17756 T17754 pobj cells,per
R13771 T17758 T17749 conj incubating,resuspending
R13773 T17760 T17759 pobj ice,on
R13775 T17762 T17763 nummod 30,min
R13777 T17764 T17763 prep in,min
R13779 T17766 T17764 pobj presence,in
R13781 T17768 T17769 compound protease,inhibitors
R13585 T17572 T17576 nmod Northern,analyses
R13586 T17573 T17572 cc and,Northern
R13587 T17574 T17572 conj Western,Northern
R13588 T17575 T17576 compound blot,analyses
R13589 T17576 T17576 ROOT analyses,analyses
R13600 T17587 T17588 advmod previously,described
R13601 T17588 T17585 advcl described,performed
R13602 T17589 T17588 punct (,described
R13603 T17590 T17588 npadvmod 29,described
R13604 T17591 T17588 punct ),described
R13605 T17592 T17585 punct .,performed
R13606 T17593 T17602 prep In,loaded
R13607 T17594 T17593 pobj brief,In
R13608 T17595 T17602 punct ",",loaded
R13609 T17596 T17597 nummod 10,μg
R13610 T17597 T17602 nsubjpass μg,loaded
R13611 T17598 T17597 prep of,μg
R13612 T17599 T17600 amod total,RNA
R13613 T17600 T17598 pobj RNA,of
R13614 T17601 T17602 auxpass was,loaded
R13615 T17602 T17602 ROOT loaded,loaded
R13616 T17603 T17602 prep per,loaded
R13617 T17604 T17603 pobj lane,per
R13618 T17605 T17602 cc and,loaded
R13619 T17606 T17602 conj transferred,loaded
R13620 T17607 T17609 aux to,charged
R13621 T17608 T17609 advmod positively,charged
R13622 T17609 T17606 xcomp charged,transferred
R13623 T17610 T17611 compound nylon,membranes
R13624 T17611 T17609 dobj membranes,charged
R13625 T17612 T17611 punct (,membranes
R13626 T17613 T17614 amod Hybond-N,+
R13627 T17614 T17611 appos +,membranes
R13628 T17615 T17611 punct ;,membranes
R13629 T17616 T17617 compound GE,Healthcare
R13630 T17617 T17611 appos Healthcare,membranes
R13631 T17618 T17611 punct ),membranes
R13632 T17619 T17611 punct ",",membranes
R13633 T17620 T17622 nsubjpass which,confirmed
R13634 T17621 T17622 auxpass was,confirmed
R16306 T21255 T21256 aux to,activate
R16161 T21110 T21109 cc and,d
R16162 T21111 T21109 conj restimulation,d
R16163 T21112 T21111 prep for,restimulation
R16164 T21113 T21114 nummod 4,h
R16165 T21114 T21112 pobj h,for
R16166 T21115 T21114 prep with,h
R16167 T21116 T21115 pobj PMA,with
R16168 T21117 T21116 cc and,PMA
R16169 T21118 T21116 conj ionomycin,PMA
R16245 T21194 T21186 pobj factors,of
R16246 T21195 T21185 punct .,diagram
R16247 T21196 T21198 nsubjpass T-bet,induced
R16248 T21197 T21198 auxpass is,induced
R16249 T21198 T21198 ROOT induced,induced
R16250 T21199 T21198 agent by,induced
R16251 T21200 T21201 compound TCR,signals
R16252 T21201 T21199 pobj signals,by
R16253 T21202 T21198 cc and,induced
R16254 T21203 T21198 conj is,induced
R16255 T21204 T21203 acomp essential,is
R16256 T21205 T21204 prep for,essential
R16257 T21206 T21208 amod early,expression
R16258 T21207 T21208 amod IFN-γ,expression
R16259 T21208 T21205 pobj expression,for
R16260 T21209 T21203 punct .,is
R16261 T21210 T21211 nsubj Runx3,is
R16262 T21211 T21211 ROOT is,is
R16263 T21212 T21211 acomp present,is
R16264 T21213 T21212 prep in,present
R16265 T21214 T21218 amod naive,cells
R16266 T21215 T21218 nmod CD8,cells
R16267 T21216 T21218 nummod +,cells
R16268 T21217 T21218 compound T,cells
R16269 T21218 T21213 pobj cells,in
R16270 T21219 T21211 cc and,is
R16271 T21220 T21211 conj represses,is
R16272 T21221 T21220 dobj Runx1,represses
R16273 T21222 T21220 cc and,represses
R16274 T21223 T21220 conj induces,represses
R16275 T21224 T21223 dobj Eomes,induces
R16276 T21225 T21224 punct ",",Eomes
R16277 T21226 T21224 appos perforin,Eomes
R16278 T21227 T21224 punct ",",Eomes
R16279 T21228 T21229 compound granzyme,B
R16280 T21229 T21224 conj B,Eomes
R16281 T21230 T21220 punct ",",represses
R16282 T21231 T21220 cc and,represses
R16283 T21232 T21233 compound IFN-γ,expression
R16284 T21233 T21220 conj expression,represses
R16285 T21234 T21211 punct .,is
R16286 T21235 T21237 nsubj Eomes,participate
R16287 T21236 T21237 aux may,participate
R16288 T21237 T21237 ROOT participate,participate
R16289 T21238 T21237 prep in,participate
R16290 T21239 T21238 pcomp sustaining,in
R16291 T21240 T21242 amod late,expression
R16292 T21241 T21242 amod IFN-γ,expression
R16293 T21242 T21239 dobj expression,sustaining
R16294 T21243 T21237 punct ",",participate
R16295 T21244 T21254 mark whereas,cooperate
R16324 T21273 T21263 cc but,indicates
R16325 T21274 T21275 neg not,granzyme
R16326 T21275 T21263 conj granzyme,indicates
R16327 T21276 T21278 compound B,expression
R16328 T21277 T21278 compound protein,expression
R16329 T21278 T21275 dobj expression,granzyme
R16330 T21279 T21263 punct .,indicates
R13713 T17700 T17699 prep from,obtained
R13715 T17702 T17704 nummod +,cells
R13716 T17703 T17704 compound T,cells
R13717 T17704 T17700 pobj cells,from
R13718 T17705 T17699 prep at,obtained
R13719 T17706 T17707 det the,time
R13720 T17707 T17705 pobj time,at
R13721 T17708 T17709 nsubj points,indicated
R13722 T17709 T17707 relcl indicated,time
R13723 T17710 T17709 prep in,indicated
R13724 T17711 T17712 det the,figures
R13725 T17712 T17710 pobj figures,in
R13726 T17713 T17709 prep during,indicated
R13727 T17714 T17715 amod clonal,expansion
R13728 T17715 T17713 pobj expansion,during
R13729 T17716 T17715 prep in,expansion
R13730 T17717 T17719 nummod 100,IL-2
R13731 T17718 T17719 compound U/ml,IL-2
R13734 T17721 T17722 compound lysis,buffer
R13735 T17722 T17720 pobj buffer,with
R13736 T17723 T17730 punct (,]
R13737 T17724 T17730 nummod 50,]
R13738 T17725 T17728 compound mM,pH
R13739 T17726 T17728 compound Tris,pH
R13740 T17727 T17728 compound [,pH
R13742 T17729 T17730 compound 7.5,]
R13744 T17731 T17730 punct ",",]
R13746 T17733 T17734 compound mM,NaCl
R13748 T17735 T17734 punct ",",NaCl
R13762 T17749 T17748 pcomp resuspending,by
R13764 T17751 T17749 prep in,resuspending
R13766 T17753 T17751 pobj μl,in
R13768 T17755 T17756 nummod 106,cells
R13770 T17757 T17749 cc and,resuspending
R13772 T17759 T17758 prep on,incubating
R13774 T17761 T17758 prep for,incubating
R13776 T17763 T17761 pobj min,for
R13778 T17765 T17766 det the,presence
R13780 T17767 T17766 prep of,presence
R13782 T17769 T17767 pobj inhibitors,of
R13590 T17577 T17576 punct .,analyses
R13591 T17578 T17579 compound RNA,isolation
R13592 T17579 T17585 nsubjpass isolation,performed
R13593 T17580 T17579 cc and,isolation
R13594 T17581 T17582 amod Northern,blot
R13595 T17582 T17583 compound blot,analysis
R13596 T17583 T17579 conj analysis,isolation
R13597 T17584 T17585 auxpass was,performed
R13598 T17585 T17585 ROOT performed,performed
R13599 T17586 T17588 mark as,described
R13714 T17701 T17704 nummod CD8,cells
R14254 T18382 T18380 conj Gzmb,Prf1
R14255 T18383 T18382 punct ",",Gzmb
R14256 T18384 T18382 conj Tbx21,Gzmb
R14257 T18385 T18386 punct (,T-bet
R14258 T18386 T18384 appos T-bet,Tbx21
R14259 T18387 T18384 punct ),Tbx21
R14260 T18388 T18384 punct ",",Tbx21
R14261 T18389 T18384 cc and,Tbx21
R14262 T18390 T18391 compound Eomes,mRNA
R14263 T18391 T18392 compound mRNA,expression
R14264 T18392 T18392 ROOT expression,expression
R14265 T18393 T18392 prep in,expression
R14266 T18394 T18393 pcomp differentiating,in
R14322 T18450 T18449 acl analyzed,cells
R14323 T18451 T18450 agent by,analyzed
R14324 T18452 T18451 pcomp immunoblotting,by
R14325 T18453 T18439 punct .,Kinetics
R14326 T18454 T18459 nsubjpass Sizes,indicated
R14327 T18455 T18454 prep of,Sizes
R14328 T18456 T18457 compound protein,bands
R14329 T18457 T18455 pobj bands,of
R14330 T18458 T18459 auxpass are,indicated
R14331 T18459 T18459 ROOT indicated,indicated
R14332 T18460 T18459 punct .,indicated
R14333 T18461 T18462 punct (,D
R14334 T18462 T18462 ROOT D,D
R14335 T18463 T18462 punct ),D
R14336 T18464 T18466 amod Relative,amounts
R14337 T18465 T18466 compound protein,amounts
R14922 T19269 T19268 prep with,restimulated
R16296 T21245 T21254 nsubj Runx3,cooperate
R16297 T21246 T21245 cc and,Runx3
R16298 T21247 T21245 conj Eomes,Runx3
R16299 T21248 T21251 punct (,T-bet
R16300 T21249 T21251 cc but,T-bet
R16301 T21250 T21251 neg not,T-bet
R16302 T21251 T21245 conj T-bet,Runx3
R16303 T21252 T21251 punct ),T-bet
R16304 T21253 T21254 aux may,cooperate
R16305 T21254 T21237 advcl cooperate,participate
R16307 T21256 T21254 advcl activate,cooperate
R16308 T21257 T21258 amod perforin,expression
R16309 T21258 T21256 dobj expression,activate
R16310 T21259 T21237 punct .,participate
R16311 T21260 T21262 det The,line
R16312 T21261 T21262 amod dotted,line
R16313 T21262 T21263 nsubj line,indicates
R16314 T21263 T21263 ROOT indicates,indicates
R16315 T21264 T21266 det the,effect
R16316 T21265 T21266 amod partial,effect
R16317 T21266 T21263 dobj effect,indicates
R16318 T21267 T21266 prep of,effect
R16319 T21268 T21269 amod T-bet,deficiency
R16320 T21269 T21267 pobj deficiency,of
R16321 T21270 T21269 prep on,deficiency
R16322 T21271 T21272 compound Gzmb,mRNA
R16323 T21272 T21270 pobj mRNA,on

UBERON-AE

Id Subject Object Predicate Lexical cue
T28 317-321 http://purl.obolibrary.org/obo/UBERON_0008915 denotes pore
T7584 21193-21210 http://purl.obolibrary.org/obo/UBERON_0001179 denotes peritoneal cavity
T11594 24168-24179 http://purl.obolibrary.org/obo/UBERON_0002371 denotes bone marrow
T14353 26556-26562 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleen
T14354 26567-26572 http://purl.obolibrary.org/obo/UBERON_0002391 denotes lymph
T14355 26567-26577 http://purl.obolibrary.org/obo/UBERON_0000029 denotes lymph node

GO-BP

Id Subject Object Predicate Lexical cue
T825 2694-2719 http://purl.obolibrary.org/obo/GO_0033077 denotes thymocyte differentiation
T826 3100-3110 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T827 3593-3612 http://purl.obolibrary.org/obo/GO_0001816 denotes cytokine production
T862 2501-2523 http://purl.obolibrary.org/obo/GO_0043379 denotes T cell differentiation
T863 1151-1170 http://purl.obolibrary.org/obo/GO_0030154 denotes cells differentiate
T864 1633-1653 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T865 2503-2523 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T866 3004-3024 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T867 3051-3071 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T868 1247-1257 http://purl.obolibrary.org/obo/GO_0006887 denotes exocytosis
T869 1402-1405 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T870 3536-3539 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T871 1489-1500 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T872 1734-1747 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T873 2433-2446 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T874 2663-2676 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T875 4224-4237 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T876 1836-1842 http://purl.obolibrary.org/obo/GO_0007613 denotes memory
T877 2032-2038 http://purl.obolibrary.org/obo/GO_0007613 denotes memory
T2690 4426-4441 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T2691 5032-5047 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T2692 4456-4476 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T2693 4506-4509 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T2694 4722-4725 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T3348 5536-5547 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T3349 5681-5696 http://purl.obolibrary.org/obo/GO_0001775 denotes cell activation
T3350 6320-6323 http://purl.obolibrary.org/obo/GO_0043715 denotes 1 E
T3351 6511-6514 http://purl.obolibrary.org/obo/GO_0043715 denotes 1 E
T3352 8518-8521 http://purl.obolibrary.org/obo/GO_0043715 denotes 1 E
T3353 6320-6323 http://purl.obolibrary.org/obo/GO_0043874 denotes 1 E
T3354 6511-6514 http://purl.obolibrary.org/obo/GO_0043874 denotes 1 E
T3355 8518-8521 http://purl.obolibrary.org/obo/GO_0043874 denotes 1 E
T3356 6407-6422 http://purl.obolibrary.org/obo/GO_0034612 denotes TNF in response
T3357 6484-6504 http://purl.obolibrary.org/obo/GO_0030154 denotes differentiated cells
T3358 7932-7942 http://purl.obolibrary.org/obo/GO_0008219 denotes cell death
T3359 7937-7942 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T3360 8007-8010 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T3361 8048-8057 http://purl.obolibrary.org/obo/GO_0019835 denotes cytolysis
T3362 8075-8090 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T3363 8492-8511 http://purl.obolibrary.org/obo/GO_0001816 denotes cytokine production
T3364 9046-9065 http://purl.obolibrary.org/obo/GO_0001816 denotes cytokine production
T3365 8624-8633 http://purl.obolibrary.org/obo/GO_0006915 denotes apoptosis
T3366 8624-8633 http://purl.obolibrary.org/obo/GO_0097194 denotes apoptosis
T4768 9211-9224 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T4769 9457-9470 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T4770 10395-10408 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T4771 9273-9283 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T4772 9616-9619 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T4773 9859-9862 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T4774 10688-10691 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T4775 10692-10699 http://purl.obolibrary.org/obo/GO_0023052 denotes signals
T4776 10844-10862 http://purl.obolibrary.org/obo/GO_1900042 denotes IL-2 and activates
T6108 11800-11809 http://purl.obolibrary.org/obo/GO_0006412 denotes translate
T7986 16218-16238 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T7987 16928-16941 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T7988 17069-17082 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T7989 17434-17447 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T7990 17580-17594 http://purl.obolibrary.org/obo/GO_0002524 denotes hypersensitive
T7991 17727-17741 http://purl.obolibrary.org/obo/GO_0002524 denotes hypersensitive
T7992 18575-18587 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T7993 18786-18796 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T7994 19078-19081 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T7995 21040-21043 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T7996 21099-21115 http://purl.obolibrary.org/obo/GO_0001906 denotes cells in killing
T11908 21632-21647 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T11909 21803-21818 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T11910 24484-24499 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T11911 25035-25048 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T13819 26014-26023 http://purl.obolibrary.org/obo/GO_0006915 denotes Apoptosis
T13820 26014-26023 http://purl.obolibrary.org/obo/GO_0097194 denotes Apoptosis
T14374 26400-26403 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T14375 27163-27166 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T16376 29752-29771 http://purl.obolibrary.org/obo/GO_0001816 denotes cytokine production
T16377 29752-29778 http://purl.obolibrary.org/obo/GO_0002369 denotes cytokine production, cells
T16378 29752-29778 http://purl.obolibrary.org/obo/GO_0002370 denotes cytokine production, cells
T16379 29752-29778 http://purl.obolibrary.org/obo/GO_0002371 denotes cytokine production, cells
T16380 29752-29778 http://purl.obolibrary.org/obo/GO_0032762 denotes cytokine production, cells
T16381 29752-29778 http://purl.obolibrary.org/obo/GO_0002368 denotes cytokine production, cells
T16382 29752-29778 http://purl.obolibrary.org/obo/GO_0035744 denotes cytokine production, cells
T16383 29752-29778 http://purl.obolibrary.org/obo/GO_0035745 denotes cytokine production, cells
T16384 30244-30246 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T16652 30364-30376 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T16653 30406-30418 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T16654 30602-30605 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T16655 30854-30856 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T16944 31333-31335 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T16945 31689-31694 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T16946 31800-31811 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T16947 32738-32748 http://purl.obolibrary.org/obo/GO_0004175 denotes proteinase
T17562 34492-34497 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T18352 6558-6573 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T18353 6588-6608 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T18354 7718-7728 http://purl.obolibrary.org/obo/GO_0006887 denotes exocytosis
T19196 11884-11894 http://purl.obolibrary.org/obo/GO_0065007 denotes Regulation
T19197 12086-12101 http://purl.obolibrary.org/obo/GO_0001775 denotes cells activated
T19198 12377-12392 http://purl.obolibrary.org/obo/GO_0001775 denotes cells activated
T19199 12535-12543 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T20181 15217-15237 http://purl.obolibrary.org/obo/GO_0030154 denotes cells differentiated
T20182 15372-15392 http://purl.obolibrary.org/obo/GO_0030154 denotes cells differentiated
T20183 15560-15580 http://purl.obolibrary.org/obo/GO_0030154 denotes cells differentiated
T20184 16041-16062 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiations
T842 1149-1170 http://purl.obolibrary.org/obo/GO_0002572 denotes T cells differentiate
T843 1631-1653 http://purl.obolibrary.org/obo/GO_0002572 denotes T cell differentiation
T844 2501-2523 http://purl.obolibrary.org/obo/GO_0002572 denotes T cell differentiation
T845 1149-1170 http://purl.obolibrary.org/obo/GO_0045580 denotes T cells differentiate
T846 1631-1653 http://purl.obolibrary.org/obo/GO_0045580 denotes T cell differentiation
T83 52-67 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T84 971-986 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T85 1059-1072 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T86 1098-1111 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T87 273-283 http://purl.obolibrary.org/obo/GO_0006887 denotes exocytosis
T847 2501-2523 http://purl.obolibrary.org/obo/GO_0045580 denotes T cell differentiation
T848 1149-1170 http://purl.obolibrary.org/obo/GO_0001865 denotes T cells differentiate
T849 1631-1653 http://purl.obolibrary.org/obo/GO_0001865 denotes T cell differentiation
T850 2501-2523 http://purl.obolibrary.org/obo/GO_0001865 denotes T cell differentiation
T851 1149-1170 http://purl.obolibrary.org/obo/GO_0030217 denotes T cells differentiate
T852 1631-1653 http://purl.obolibrary.org/obo/GO_0030217 denotes T cell differentiation
T853 2501-2523 http://purl.obolibrary.org/obo/GO_0030217 denotes T cell differentiation
T854 1149-1170 http://purl.obolibrary.org/obo/GO_0042093 denotes T cells differentiate
T855 1631-1653 http://purl.obolibrary.org/obo/GO_0042093 denotes T cell differentiation
T856 2501-2523 http://purl.obolibrary.org/obo/GO_0042093 denotes T cell differentiation
T857 1149-1170 http://purl.obolibrary.org/obo/GO_0045066 denotes T cells differentiate
T858 1631-1653 http://purl.obolibrary.org/obo/GO_0045066 denotes T cell differentiation
T859 2501-2523 http://purl.obolibrary.org/obo/GO_0045066 denotes T cell differentiation
T860 1149-1170 http://purl.obolibrary.org/obo/GO_0043379 denotes T cells differentiate
T861 1631-1653 http://purl.obolibrary.org/obo/GO_0043379 denotes T cell differentiation
T15053 27967-27970 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T15054 28058-28061 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T15055 28265-28280 http://purl.obolibrary.org/obo/GO_0001775 denotes activated cells
T15056 28295-28305 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T20963 19835-19850 http://purl.obolibrary.org/obo/GO_2000144 denotes transactivation
T20964 20347-20362 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T20965 20426-20429 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T20966 20430-20437 http://purl.obolibrary.org/obo/GO_0023052 denotes signals
T12715 25195-25208 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T12716 21632-21665 http://purl.obolibrary.org/obo/GO_0045896 denotes transcriptional regulation during
T12717 21648-21658 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T12718 21819-21829 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T12719 23786-23796 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T12720 23853-23863 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T12721 22145-22160 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T12722 24604-24611 http://purl.obolibrary.org/obo/GO_0023052 denotes signals
T12723 24893-24900 http://purl.obolibrary.org/obo/GO_0023052 denotes signals
T12724 24889-24892 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T12725 25241-25249 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular

GO-MF

Id Subject Object Predicate Lexical cue
T4292 6320-6323 http://purl.obolibrary.org/obo/GO_0043715 denotes 1 E
T4293 6511-6514 http://purl.obolibrary.org/obo/GO_0043715 denotes 1 E
T4294 8518-8521 http://purl.obolibrary.org/obo/GO_0043715 denotes 1 E
T4295 6320-6323 http://purl.obolibrary.org/obo/GO_0043874 denotes 1 E
T4296 6511-6514 http://purl.obolibrary.org/obo/GO_0043874 denotes 1 E
T4297 8518-8521 http://purl.obolibrary.org/obo/GO_0043874 denotes 1 E
T4298 8988-8992 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T5502 10844-10848 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T16634 30126-30136 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T16635 30244-30246 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T14220 25339-25349 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T14221 25693-25703 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T17518 31333-31335 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T17519 31800-31811 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T17520 32062-32070 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T17521 32738-32748 http://purl.obolibrary.org/obo/GO_0004175 denotes proteinase
T2073 2651-2662 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA-binding
T2074 2655-2662 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T2075 2655-2684 http://purl.obolibrary.org/obo/GO_0008134 denotes binding transcription factors
T2076 2655-2684 http://purl.obolibrary.org/obo/GO_0033613 denotes binding transcription factors
T2077 2655-2684 http://purl.obolibrary.org/obo/GO_0043425 denotes binding transcription factors
T2078 2655-2684 http://purl.obolibrary.org/obo/GO_0070491 denotes binding transcription factors
T3138 4699-4703 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T10031 14992-14996 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T10032 17478-17482 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T10033 16403-16407 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T10034 17852-17874 http://purl.obolibrary.org/obo/GO_0035326 denotes enhancer; this binding
T10035 17867-17874 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T10036 21562-21568 http://purl.obolibrary.org/obo/GO_0005488 denotes ligand
T12726 22338-22342 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T12727 22386-22390 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T14222 25836-25844 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T14223 25428-25432 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T15774 28028-28032 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T15775 28421-28425 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T16913 30854-30856 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T18085 33917-33930 http://purl.obolibrary.org/obo/GO_0003735 denotes ribosomal RNA
T18086 34482-34486 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T18087 34738-34748 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T20465 15487-15491 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T20466 15748-15755 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T21621 19741-19749 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody

GO-CC

Id Subject Object Predicate Lexical cue
T471 131-136 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T472 264-269 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T473 317-321 http://purl.obolibrary.org/obo/GO_0046930 denotes pore
T2079 1151-1156 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2080 1238-1243 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2081 2005-2010 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2082 2099-2104 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2083 2895-2900 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2084 2955-2960 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2085 3224-3229 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2086 3267-3272 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2087 3872-3877 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2088 3881-3886 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2089 1402-1405 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T2090 3536-3539 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T3144 4876-4881 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3145 5264-5269 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3146 5436-5441 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3147 4506-4509 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T3148 4722-4725 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T3149 5090-5093 http://purl.obolibrary.org/obo/GO_0005680 denotes APC
T6898 11094-11099 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6899 11186-11191 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6900 11339-11344 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6901 11564-11569 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6902 11745-11750 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6903 13187-13192 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6904 13548-13553 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6905 13694-13699 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6906 13797-13802 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6907 13257-13265 http://purl.obolibrary.org/obo/GO_0005840 denotes ribosome
T10037 14060-14065 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10038 14140-14145 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10039 14234-14239 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10040 14493-14498 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10041 14664-14669 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10042 14752-14757 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10043 14808-14813 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10044 15008-15013 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10045 16704-16709 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10046 17692-17697 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14230 25359-25372 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T14231 26138-26142 http://purl.obolibrary.org/obo/GO_0019013 denotes Core
T14232 26243-26247 http://purl.obolibrary.org/obo/GO_0019013 denotes Core
T14827 26350-26355 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14828 26364-26369 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14829 26578-26583 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14830 26592-26597 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14831 26933-26938 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14832 26960-26965 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14833 27135-27140 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14834 27224-27229 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14835 27296-27301 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14836 27341-27346 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14837 26400-26403 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T14838 27163-27166 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T15776 27435-27440 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15777 27479-27484 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15778 27610-27615 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15779 27667-27672 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15780 27795-27800 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15781 27861-27866 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15782 27882-27887 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15783 28103-28108 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15784 28205-28210 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15785 28275-28280 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15786 28402-28407 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15787 28554-28559 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15788 28604-28609 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15789 28671-28676 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15790 28948-28953 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15791 29075-29080 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15792 29147-29152 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15793 27967-27970 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T15794 28058-28061 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T15795 28218-28230 http://purl.obolibrary.org/obo/GO_0009986 denotes cell-surface
T16332 29230-29235 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16333 29326-29331 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16334 29453-29458 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16335 29601-29606 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16336 29681-29686 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16914 30395-30400 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16915 30511-30516 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16916 30631-30636 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16917 30602-30605 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T18088 33827-33836 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T18089 33946-33954 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T18090 33956-33965 http://purl.obolibrary.org/obo/GO_0016020 denotes Membranes
T18091 33917-33930 http://purl.obolibrary.org/obo/GO_0005840 denotes ribosomal RNA
T18092 34349-34353 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T18093 34396-34401 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18094 34616-34621 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18095 34738-34748 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T18096 34738-34748 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T18899 6588-6592 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T18900 6709-6714 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18901 6765-6770 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18902 6972-6977 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18903 7368-7373 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18904 7515-7520 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18905 7112-7125 http://purl.obolibrary.org/obo/GO_0005622 denotes Intracellular
T19748 12056-12061 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19749 12076-12081 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19750 12086-12091 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19751 12278-12283 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19752 12377-12382 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19753 12610-12615 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19754 12681-12686 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19755 12766-12771 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19756 12797-12802 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19757 12859-12864 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19758 13036-13041 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19759 12216-12229 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T20467 15217-15222 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T20468 15372-15377 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T20469 15560-15565 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T20470 15654-15659 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T20471 16041-16045 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T20472 15921-15930 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T20473 15990-15999 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T21622 19317-19322 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T21623 19500-19505 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T21624 19570-19575 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T21625 20048-20053 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T21626 20243-20248 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T21627 20516-20521 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T21628 19741-19749 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T21629 19741-19749 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T21630 19987-20000 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T21631 20426-20429 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T4299 5681-5685 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T4300 7932-7936 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T4301 8131-8136 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4302 8390-8395 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4303 8529-8534 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4304 8662-8667 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4305 8007-8010 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T5503 9578-9583 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5504 9848-9853 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5505 9616-9619 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T5506 9859-9862 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T5507 10688-10691 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T14224 25339-25349 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T14225 25693-25703 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T14226 25836-25844 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T16636 29773-29778 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16637 30004-30009 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16638 29886-29899 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T16639 30126-30136 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T16640 30126-30136 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T17522 31106-31111 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17523 31402-31407 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17524 31672-31677 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17525 32093-32097 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T17526 33577-33581 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T17527 31829-31838 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T17528 31931-31940 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T17529 31997-32006 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T17530 32639-32648 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T17531 33523-33532 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T17532 32062-32070 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T17533 32062-32070 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T14227 25339-25349 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T14228 25693-25703 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T14229 25836-25844 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T3139 4293-4297 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T3140 4370-4374 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T3141 4456-4460 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T3142 4491-4496 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3143 4813-4818 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10047 18096-18101 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10048 18280-18285 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10049 18346-18351 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10050 18445-18450 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10051 18510-18515 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10052 18623-18628 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10053 18755-18760 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10054 19002-19007 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10055 20952-20957 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10056 21069-21074 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10057 21099-21104 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10058 21122-21127 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10059 21178-21183 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10060 21257-21262 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10061 21400-21405 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10062 21413-21418 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10063 17177-17186 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T10064 19078-19081 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T10065 21040-21043 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T12728 21898-21903 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12729 22524-22529 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12730 22827-22832 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12731 23008-23013 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12732 23344-23349 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12733 23430-23435 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12734 24072-24077 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12735 24142-24147 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12736 24209-24214 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12737 24349-24354 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12738 24794-24799 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12739 24862-24867 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12740 24978-24983 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12741 24889-24892 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T18254 35109-35114 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18255 35196-35201 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T828 1138-1413 Sentence denotes Naive CD8+ T cells differentiate into effector CTLs with the ability to lyse antigen-bearing target cells by exocytosis of lytic granules containing perforin and granzymes, and to produce inflammatory cytokines such as IFN-γ and TNF upon restimulation through the TCR (1, 2).
T829 1414-1547 Sentence denotes In vivo experiments have elucidated many critical parameters governing the development and evolution of primary CTL responses (3, 4).
T830 1548-1867 Sentence denotes In this study, we have used in vitro systems such as those developed to study CD4+ T cell differentiation to define the molecular basis of effector CTL differentiation (5, 6). The T-box transcription factors Eomesodermin (Eomes) and T-bet are needed for important aspects of effector and memory CTL differentiation (7).
T831 1868-2053 Sentence denotes In uninfected mice, compound deletion of the Tbx21 (encoding T-bet) and eomesodermin genes is associated with a selective loss of CD8+ T cells with an IL-2Rβ–high, memory phenotype (8).
T832 2054-2263 Sentence denotes Mice deficient for both T-bet and Eomes in T cells have impaired expression of cytolytic mediators, manifest poor cytolytic activity, and fail to control acute lymphocytic choriomeningitis virus infection (9).
T833 2264-2761 Sentence denotes Nevertheless, the specific roles of T-bet and Eomes in clonal expansion and CTL differentiation have not yet been resolved: in particular, it is not known whether these transcription factors function redundantly to control effector CD8+ T cell differentiation, and whether they do so directly by targeting specific effector cytokine and cytolytic genes. Runx proteins, a family of three DNA-binding transcription factors, control thymocyte differentiation and the CD4/CD8 lineage decision (10–13).
T834 2762-2906 Sentence denotes Runx3 and perforin mRNA are expressed by double-positive (DP) thymocytes and CD8+ single-positive (SP) thymocytes but not in CD4+ SP cells (14).
T835 2907-3155 Sentence denotes Although Runx3 is not expressed in naive CD4+ T cells, its expression is up-regulated during Th1 cell differentiation, and Runx3 influences Th1 cell differentiation and function through direct regulation of the Il4 and Ifng cytokine genes (15, 16).
T836 3156-3314 Sentence denotes In contrast, all three Runx proteins are expressed in mature CD8+ T cells (10, 12), and Runx3-deficient CD8+ T cells show reduced cytolytic activity (12, 13).
T837 3315-3505 Sentence denotes We therefore tested whether Runx3 influenced cytolytic T cell differentiation. In this report, we show that Runx3 and T-box factors synergistically regulate CTL differentiation and function.
T838 3506-3703 Sentence denotes T-bet is induced quickly upon TCR stimulation and is required for early programming of cytokine production (17), whereas Eomes is induced later during differentiation and sustains IFN-γ expression.
T839 3704-3878 Sentence denotes Runx3 is required for Eomes and perforin expression, and both Eomes and Runx3 bind at the Prf1 locus; in contrast, perforin expression is unaffected in T-bet–deficient cells.
T840 3879-4021 Sentence denotes T cells lacking Runx3 show decreased expression of IFN-γ and granzyme B, and Runx3 also binds the promoter regions of the Ifng and Gzmb genes.
T841 4022-4265 Sentence denotes Collectively, these results provide evidence for a complex transcriptional network in which Runx3 is a primary regulator of Gzmb expression but synergizes with T-bet and Eomes, respectively, to promote transcription of the Ifng and Prf1 genes.
T3367 5469-5558 Sentence denotes Distinct expression kinetics of perforin and granzyme B during CTL development in culture
T3368 5559-5706 Sentence denotes Our experiments revealed clear differences in the kinetics of perforin, granzyme B, and cytokine expression during CD8+ T cell activation (Fig. 1).
T3369 5707-5809 Sentence denotes Naive T cells showed detectable expression of perforin mRNA as well as perforin protein (Fig. 1, A–D).
T3370 5810-6037 Sentence denotes Relative to its expression in naive T cells, perforin (Prf1) mRNA expression did not increase appreciably at day 2 but showed a reproducible decrease at day 4, followed by robust reexpression between days 4 and 8 (Fig. 1, A–D).
T3371 6038-6325 Sentence denotes In contrast, granzyme B (Gzmb) mRNA was low or undetectable in naive T cells but was strongly up-regulated by day 2 after stimulation and increased progressively until day 6 (Fig. 1, A and B); similarly, granzyme B protein was expressed by day 4 and remained high until day 6 (Fig. 1 E).
T3372 6326-6535 Sentence denotes As expected, a small fraction of naive T cells expressed the cytokines IFN-γ and TNF in response to stimulation, and this capacity increased significantly in differentiated cells (Fig. 1 E; see also Fig. 2 A).
T3373 7844-7978 Sentence denotes We evaluated antigen-dependent cytolytic function in a short-term assay in which target cell death was measured within 2 h (Fig. 1 F).
T3374 7979-8122 Sentence denotes By limiting the duration of TCR stimulation, this strategy minimizes cytolysis secondary to new gene expression during the period of the assay.
T3375 8123-8349 Sentence denotes Naive T cells did not display significant cytolytic function in this short-term assay (unpublished data), most likely because they express immature (unprocessed) forms of perforin and lack the capacity to degranulate (18, 19).
T3376 8350-8523 Sentence denotes Even after activation for 2 or 4 d, the cells showed poor cytolytic activity (Fig. 1 F), in striking contrast to their capacity for efficient cytokine production (Fig. 1 E).
T3377 8524-8679 Sentence denotes Only cells cultured until day 6 displayed robust cytotoxicity, as judged by their ability to induce apoptosis in a large number of target cells (Fig. 1 F).
T3378 8680-8993 Sentence denotes These results show that after a strong priming stimulus through TCRs and co-stimulatory receptors in vitro, granzyme B expression and the ability to produce effector cytokines are programmed early, whereas perforin expression and cytolytic function are induced later, during the phase of clonal expansion in IL-2.
T3379 8994-9124 Sentence denotes Therefore, the two major effector functions of CTL, cytokine production and cytolytic activity, are not intrinsically coregulated.
T6114 11533-11666 Sentence denotes In contrast, T-bet–deficient T cells cultured for 6 d showed no defect in perforin mRNA expression (Fig. 2 B, compare lanes 1 and 4).
T6115 11667-11873 Sentence denotes We consistently observed a modest reduction in GzmB mRNA in T-bet–deficient T cells (Fig. 2 B, compare lanes 1 and 4), which did not translate into a decrease in expression of granzyme B protein (Fig. 2 C).
T6116 13130-13436 Sentence denotes To examine the role of Eomes, we transduced naive CD8+ T cells from WT and Tbx21−/− mice with retroviruses containing internal ribosome entry site (IRES)–GFP that were either empty or encoded a strongly transactivating version of Eomes (Eo-VP16) (8), and expanded them for 6 d under our culture conditions.
T6117 13437-13587 Sentence denotes Eo-VP16, but not the empty GFP retrovirus, increased perforin expression in both WT and T-bet–deficient CD8+ T cells (Fig. 2 B, lanes 2, 3, 5, and 6).
T6118 13588-13711 Sentence denotes As expected, Eo-VP16 also rescued the early defect in IFN-γ production observed in T-bet–deficient CD8+ T cells (Fig. 2 D).
T6119 13712-13900 Sentence denotes However, Eo-VP16 did not induce GzmB mRNA expression in either WT or T-bet–deficient cells; thus, the partial T-bet dependence of GzmB mRNA expression cannot be compensated for by Eo-VP16.
T7964 14215-14464 Sentence denotes We isolated CD8+ T cells from Runx3−/− (KO) mice of the outbred ICR background and their WT Runx3+/+ littermates by positive selection with anti-CD8 magnetic beads (Figs. S1 and S2, available at http://www.jem.org/cgi/content/full/jem.20081242/DC1).
T7965 14465-14644 Sentence denotes Strikingly, Runx3−/− CD8+ T cells were strongly impaired in their ability to differentiate into effector CTLs, as judged by expression of perforin, granzyme B, and IFN-γ (Fig. 3).
T7966 14645-14796 Sentence denotes Compared with WT T cells, perforin mRNA and protein expression were essentially undetectable in Runx3−/− T cells at day 6 of culture (Fig. 3, A and B).
T7967 14797-14911 Sentence denotes Runx3−/− T cells also had no detectable Eomes expression; in contrast, T-bet expression was unimpaired (Fig. 3 A).
T7968 14912-15047 Sentence denotes Furthermore, Runx3 was required for maximal production of IFN-γ, but not TNF or IL-2, by CD8+ T cells restimulated at day 6 (Fig. 3 C).
T7969 16186-16479 Sentence denotes We previously reported that Th1 cell differentiation was regulated through a feed-forward loop in which T-bet is up-regulated early and induces Runx3, after which T-bet and Runx3 cooperate to induce IFN-γ and silence IL-4, thus promoting stable differentiation toward the Th1 lineage (15, 22).
T7970 16480-16950 Sentence denotes Because (a) Runx3 appeared necessary for Eomes induction (Fig. 3 A), (b) the kinetics of Eomes expression paralleled those of perforin expression (Fig. 2), and (c) overexpression of Eo-VP16 in either WT or T-bet–deficient T cells led to an increase in both perforin and IFN-γ expression (Fig. 2, B and D), we asked whether CTL differentiation was also potentially regulated by a feed-forward loop involving these same two classes of Runx and T-box transcription factors.
T7971 16951-17143 Sentence denotes Specifically, we asked whether Runx3, which was necessary for Eomes induction, then cooperated with Eomes to regulate transcription of the effector CTL markers perforin, IFN-γ, and granzyme B.
T7972 17144-17322 Sentence denotes To test this hypothesis, we used chromatin immunoprecipitation (ChIP) assays to ask whether Eomes and Runx3 bound regulatory regions of the Prf1, Ifng, and Gzmb genes (Fig. 3 D).
T7973 17323-17400 Sentence denotes Both proteins associated with gene regulatory regions in differentiated CTLs.
T7974 17401-17799 Sentence denotes Runx3 bound to the Prf1 and Gzmb transcription start sites (TSS); to a known IL-2 responsive enhancer located near −1 kb of the Prf1 gene (23); to the distal CTL-specific DNase I hypersensitive site 9 in the Prf1 locus (24); to the Ifng promoter near the TSS, as previously reported for Th1 cells (10); and to several DNase I hypersensitive sites in the Ifng locus (Fig. 3 D and not depicted) (25).
T7975 17800-18118 Sentence denotes Eomes bound primarily to the Prf1 TSS and the −1 kb enhancer; this binding was substantially greater than that observed at the promoter of the Il2rb gene, a known direct target of Eomes (8), and comparable to that observed at the Ifng TSS, a known target of T-box proteins in both Th1 and CD8+ T cells (Fig. 3 D) (17).
T7976 18119-18305 Sentence denotes To determine whether Runx3 controlled the expression of CTL effector genes through its induction of Eomes, we retrovirally expressed Runx3 and Eo-VP16 in CD8+ T cells from Runx3−/− mice.
T7977 18306-18588 Sentence denotes Because of the limited number of CD8+ T cells in these mice, and because we saw no difference between Runx3−/− CD8+CD4− SP and CD8+CD4+ DP cells in our previous experiments, we used total Runx3−/− CD8+ T cells without further fractionation as recipients for retroviral transduction.
T7978 18589-18730 Sentence denotes Reconstitution of Runx3−/− CD8+ T cells with Runx3 restored expression of Eomes as well as perforin, granzyme B, and IFN-γ (Fig. 4, A and B).
T7979 18731-18917 Sentence denotes In addition, Runx3−/− T cells showed a compensatory up-regulation of Runx1, which was suppressed upon reconstitution with Runx3, indicating that Runx1 is a target of repression by Runx3.
T7980 18918-19114 Sentence denotes Notably, Eo-VP16 did not up-regulate perforin expression when expressed in Runx3−/− cells, even though it restored the capacity to induce IFN-γ expression upon TCR restimulation (Fig. 4, A and B).
T7981 19115-19195 Sentence denotes This result suggests strongly that perforin expression requires Runx3 and Eomes.
T7982 20867-21030 Sentence denotes As expected from their defect in perforin and granzyme B expression, Runx3−/− CD8+ T cells showed defective cytolytic activity in a mixed lymphocyte reaction (12).
T7983 21031-21159 Sentence denotes However, TCR-stimulated Runx3−/− CD8+ cells were as effective as WT cells in killing tumor cells in a redirected CTL assay (12).
T7984 21160-21301 Sentence denotes Furthermore, CD8+ cells from the peritoneal cavity of Runx3−/− mice immunized with certain tumor cells effectively killed these targets (13).
T7985 21302-21577 Sentence denotes Therefore, although activation of the perforin/granzyme B machinery is defective in Runx3−/− CD8+ cells, these cells are not entirely devoid of cytolytic activity and could still effectively kill targets, possibly by alternative mechanisms such as the Fas–Fas ligand pathway.
T11889 21579-21685 Sentence denotes Runx3 and T-box factors control a complex program of transcriptional regulation during CTL differentiation
T11890 21686-21864 Sentence denotes Collectively, these data provide evidence that Runx3, together with T-box factors, orchestrates a complex program of transcriptional regulation in differentiating CTL (Fig. 4 C).
T11891 21865-21927 Sentence denotes Runx3 is present in naive CD8+ T cells before activation (12).
T11892 21928-22030 Sentence denotes It represses Runx1 and has a positive role in the induction of Eomes, granzyme B, perforin, and IFN-γ.
T11893 22031-22161 Sentence denotes Runx3 binds to promoters and putative regulatory regions of the latter three genes, suggesting a direct effect on gene expression.
T11894 22162-22271 Sentence denotes Additional experiments are needed to determine whether Eomes and Runx1 are also direct target genes of Runx3.
T11895 22272-22373 Sentence denotes Surprisingly, Runx3 contributed to the optimal expression of TNF, IL-2, and IFN-γ at day 4 (Fig. S2).
T11896 22374-22541 Sentence denotes For TNF and IL-2, the requirement for Runx3 subsides by day 6 (Fig. 3 C), possibly because of compensation by Runx1, which is derepressed in Runx3−/− cells (Fig. 4 A).
T11897 22542-22685 Sentence denotes Runx3 continues to be required for IFN-γ expression even at day 6, perhaps because of its role in the induction of Eomes expression (Fig. 4 A).
T11898 22686-22955 Sentence denotes An unexpected finding was that the two T-box transcription factors, T-bet and Eomes, are up-regulated with very different kinetics in CD8+ T cells under our culture conditions and have nonredundant roles in the subsequent expression of key effector proteins (Fig. 4 C).
T11899 22956-23116 Sentence denotes T-bet is needed early to confer on activated CD8+ T cells the competence to produce IFN-γ upon restimulation, but its function is less important at later times.
T11900 23117-23274 Sentence denotes Eomes, which is induced late and functions downstream of Runx3, may substitute for T-bet in promoting the acute expression of IFN-γ in restimulated CTLs (8).
T11901 23275-23531 Sentence denotes Indeed, T-bet and Eomes both contribute to perforin expression in NK cells (8, 26), and Eomes induces granzyme B as effectively as T-bet in developing Th2 cells (7); thus, the relative roles of these T-box transcription factors vary depending on cell type.
T11902 23532-23680 Sentence denotes Surprisingly, however, Eomes and T-bet appeared nonredundant in their ability to induce two other markers of CTL function, Prf1 and Gzmb (Fig. 4 C).
T11903 23681-23912 Sentence denotes Rather, T-bet and Eomes were involved in regulating granzyme B and perforin expression, respectively: up-regulation of T-bet and Eomes mRNA and protein closely preceded up-regulation of Gzmb and Prf1 mRNA and protein, respectively.
T11904 23913-24078 Sentence denotes T-bet had no role in perforin expression under our culture conditions, and Eo-VP16 did not affect granzyme B expression when expressed in T-bet−/− or Runx3−/− cells.
T11905 24079-24438 Sentence denotes Because conventional Eomes-deficient mice die before precursor cells can be isolated for bone marrow transfers (27) and because T cells conditionally deficient in Eomes have only recently been described (9), we were unable to introduce Runx3 into Eomes-deficient CD8+ T cells to test formally whether Eomes cooperated with Runx3 to induce perforin expression.
T11906 24439-24677 Sentence denotes Collectively, our data are consistent with a transcriptional network in which preexisting Runx3 cooperates with the induced T-box factors T-bet and Eomes and IL-2Rβ signals (unpublished data) to orchestrate CTL differentiation (Fig. 4 C).
T11907 24678-25275 Sentence denotes Our data recall the “feed-forward” interaction between T-bet and Runx3 that we previously described in CD4+ (Th1) T cells (15) but are distinct in two respects: in differentiating Th1 cells, T-bet is induced by TCR signals and IFN-γ, and in turn induces Runx3 (15), whereas in differentiating CD8+ T cells, preexisting Runx3 is required to induce the T-box transcription factor Eomes. Whole-genome experiments in these and other systems will be required to establish whether cooperation between T-box and Runx family transcription factors is a general feature of cellular differentiation programs.
T13821 25300-25324 Sentence denotes Antibodies and reagents.
T13822 25325-25508 Sentence denotes The following antibodies used for intracellular or surface stains were obtained from eBioscience: anti–IL-2, anti–IFN-γ, anti-TNF, anti–granzyme B, anti-CD8, anti-CD25, and anti-CD44.
T13823 25509-25541 Sentence denotes Anti-CD69 was purchased from BD.
T13824 25542-25678 Sentence denotes For ChIP experiments, the anti-Eomes antibody was obtained from Abcam and the anti-Runx3 antibody was produced by the Groner laboratory.
T13825 25679-25825 Sentence denotes The following antibodies were used for immunoblotting: antiperforin (Abcam), anti-Eomes (Abcam), and anti–Pol-II (Santa Cruz Biotechnology, Inc.).
T13826 25826-25919 Sentence denotes The T-bet antibody was provided by L. Glimcher (Harvard School of Public Health, Boston, MA).
T13827 25920-25998 Sentence denotes The following reagents were used for the experiments presented in this report:
T13828 25999-26173 Sentence denotes Annexin V–FITC Apoptosis Detection Kit (BD), CD8 Negative Isolation Kit (Invitrogen), CD8 MicroBeads (Miltenyi Biotec), and SYBR Green PCR Core Reagents (Applied Biosystems).
T13829 26174-26308 Sentence denotes The Gp33 peptide (KAVYNFATC) was synthesized by the Tufts University Core Facility, and 10 mM of stock solutions was prepared in DMSO.
T15043 27415-27460 Sentence denotes Isolation of CD8+ T cells from Runx3−/− mice.
T15044 27461-27544 Sentence denotes Runx3-deficient T cells fail to silence CD4 expression normally (Fig. S1) (12, 13).
T15045 27545-27717 Sentence denotes We therefore further fractionated the positively selected CD8+ T cells from Runx3 KO mice into CD8+CD4− SP or CD8+CD4+ DP cells by separation using anti-CD4 magnetic beads.
T15046 27718-27877 Sentence denotes This yielded a Runx3 KO SP “enriched” population that contained 75% CD8+CD4− cells and a KO DP enriched population that contained 85% CD8+CD4+ cells (Fig. S1).
T15047 27878-28033 Sentence denotes The cells were stimulated with anti-CD3+ anti-CD28 for 2 d before removing them from the TCR stimulus and culturing them in media containing 100 U/ml IL-2.
T15048 28034-28182 Sentence denotes As previously reported, TCR-induced proliferation of Runx3−/− CD8+ T cells was severely impaired, irrespective of CD4 expression (Fig. S1) (12, 13).
T15049 28183-28333 Sentence denotes However, the Runx3−/− cells showed cell-surface expression patterns indicative of activated cells, including up-regulation of CD25 and CD69 (Fig. S1).
T15050 28334-28570 Sentence denotes As expected from their ability to up-regulate CD25, Runx3−/− CD8+ T cells responded to IL-2 supplementation after day 2 and efficiently expanded until day 6 of the culture period, albeit at slower rates compared with WT cells (Fig. S1).
T15051 28571-29023 Sentence denotes Although a fraction of the KO DP cells silenced CD4 expression after activation, the ratio of SP/DP cells in each enriched population remained constant thereafter, and we did not observe any major differences between these two populations throughout the culture period, indicating that in terms of effector CTL differentiation and under our culture conditions, Runx3−/− CD8+ T cells that also coexpress CD4 are indistinguishable from those that do not.
T15052 29024-29153 Sentence denotes The data presented in Fig. S2 are from Runx3 KO SP cells, whereas those shown in Figs. 3 and 4 are from total Runx3 KO CD8 cells.
T16126 29155-29185 Sentence denotes FACS-based cytotoxicity assay.
T16127 29186-29421 Sentence denotes To measure cytotoxicity, EL4 thymoma target cells were loaded with 0 or 1 μM Gp33 peptide for 2 h before a 2-h coincubation with P14 CD8+ T cells at the effector-to-target ratios indicated in the figures in 96-well round-bottom plates.
T16128 29422-29521 Sentence denotes After the coincubation period, cells were stained with Annexin V–FITC and anti-CD8–allophycocyanin.
T16129 29522-29702 Sentence denotes Data analysis was performed with FlowJo software (Tree Star, Inc.); EL4 target cells (CD8-negative events) were gated, and the percentage of Annexin V+ target cells was determined.
T16373 29704-29741 Sentence denotes Cytokine and surface marker staining.
T16374 29742-29960 Sentence denotes To assess cytokine production, cells were restimulated with 10 nM PMA + 1 μM ionomycin for 6 h (unless indicated otherwise in the figures), and intracellular cytokine stains were performed as previously described (28).
T16375 29961-30351 Sentence denotes To detect expression of surface molecules, cells were washed in PBS, resuspended in FACS wash buffer (3% FBS, 0.1% sodium azide, 30 mM Hepes, 1× PBS) containing the antibodies indicated in the figures at previously optimized concentrations, incubated for 15 min at room temperature (RT), washed, and resuspended in 2% formaldehyde fixative solution before acquisition on a FACSCalibur (BD).
T16647 30353-30401 Sentence denotes Retroviral transduction of primary CD8+ T cells.
T16648 30402-30574 Sentence denotes For transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g.
T16649 30575-30805 Sentence denotes At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus.
T16650 30806-30904 Sentence denotes The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h.
T16651 30905-31055 Sentence denotes Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8).
T16948 31057-31089 Sentence denotes ChIP and real-time PCR analysis.
T16949 31090-31336 Sentence denotes 20 × 106 CD8+ T cells per immunoprecipitation were fixed by adding a 1/10th volume of fixation solution (11.1% formaldehyde, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 50 mM Hepes) to 1 volume of culture media and were incubated for 10 or 30 min at RT.
T16950 31337-31395 Sentence denotes Fixation was stopped with 120 mM glycine on ice for 5 min.
T16951 31396-31615 Sentence denotes Fixed cells were washed 2× with cold PBS, 1× with cold solution I (10 mM Tris [pH 7.5], 10 mM EDTA, 0.5 mM EGTA, 1% Triton X-100), and 1× with cold solution II (10 mM Tris [pH 7.5], 1 mM EDTA, 0.5 mM EGTA, 200 mM NaCl).
T16952 31616-31900 Sentence denotes After washes, cell pellets were resuspended at 40 × 106 cells/ml in ChIP lysis buffer (150 mM NaCl, 25 mM Tris [pH 7.5], 1% Triton X-100, 0.1% SDS, 0.5% deoxycholate plus protease and phosphatase inhibitors), and chromatin was sheared with a sonicator to yield 0.5–1-kb DNA fragments.
T16953 31901-32110 Sentence denotes After preclearing the sheared chromatin with protein A–sepharose beads and removing 5% as input chromatin, immunoprecipitation was performed by adding optimized antibody amounts (per 20 × 106 cell equivalents:
T16954 32111-32278 Sentence denotes 2.5 μg anti-Eomes, 1:100 dilution anti-Runx3), followed by overnight incubation at 4°C; protein A–sepharose beads were added for the last 3 h of the incubation period.
T16955 32279-32513 Sentence denotes Beads were washed 2× with RIPA buffer (50 mM Tris [pH 8], 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% deoxycholate), 1× with high salt buffer (50 mM Tris [pH 8], 500 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS), and 1× with TE buffer.
T16956 32514-32618 Sentence denotes After the last wash, DNA was eluted by resuspending the beads in elution buffer (1% SDS, 100 mM NaHCO3).
T16957 32619-32824 Sentence denotes Both input and ChIP chromatin were then treated with RNase A (5 μg total) for 1 h at 37°C, followed by the addition of proteinase K (100 μg total) and overnight incubation at 65°C to reverse cross-linking.
T16958 32825-32977 Sentence denotes DNA was then purified with QIAquick columns (Gel Extraction Kit; QIAGEN) according to the manufacturer's instructions and resuspended in a 50-μl volume.
T16959 32978-33218 Sentence denotes For real-time PCR detection of immunoprecipitated targets using the SYBR Green PCR Kit, a standard curve was obtained with serial dilutions of input DNA for each sample, and 1 μl ChIP DNA was used per PCR reaction (performed in duplicates).
T16960 33219-33315 Sentence denotes Melt curves and agarose gels were analyzed to ensure amplification of specific target sequences.
T16961 33316-33428 Sentence denotes Refer to Table S1 (available at http://www.jem.org/cgi/content/full/jem.20081242/DC1) for a list of primer sets.
T16962 33429-33611 Sentence denotes The data are presented as the number of immunoprecipitated target sequences relative to input chromatin, assuming two copies of target sequence per cell equivalent used for the ChIP.
T17563 33613-33648 Sentence denotes Northern and Western blot analyses.
T17564 33649-33733 Sentence denotes RNA isolation and Northern blot analysis was performed as previously described (29).
T17565 33734-33955 Sentence denotes In brief, 10 μg of total RNA was loaded per lane and transferred to positively charged nylon membranes (Hybond-N+; GE Healthcare), which was confirmed by ethidium bromide staining of ribosomal RNA species on the membrane.
T17566 33956-34120 Sentence denotes Membranes were hybridized with 1 ng/ml α-[32P]dCTP–labeled trichloroacetic acid precipitable probe in ExpressHyb hybridization buffer (Clontech Laboratories, Inc.).
T17567 34121-34260 Sentence denotes All cDNA probes were confirmed to have the appropriate single-copy specificity under these conditions using genomic Southern blot analysis.
T17568 34261-34320 Sentence denotes Band intensities were acquired by phosphorimaging analysis.
T17569 34321-34694 Sentence denotes For Western analysis, whole-cell protein lysates were obtained from CD8+ T cells at the time points indicated in the figures during clonal expansion in 100 U/ml IL-2 with lysis buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 10% glycerol, 5 mM EDTA, 1% NP-40) by resuspending samples in 10 μl per 106 cells and incubating on ice for 30 min in the presence of protease inhibitors.
T17570 34695-34837 Sentence denotes Immunoblot analysis was performed with the antibodies indicated in the figures after SDS-PAGE (10–30 μg of total protein was loaded per well).
T17571 34838-35021 Sentence denotes Quantification of detected protein was performed with an Intelligent Dark Box unit (LAS-3000; Fujifilm) and normalized for loading with the amount of RNA Pol-II detected in each lane.
T18121 35023-35052 Sentence denotes Online supplemental material.
T18122 35053-35134 Sentence denotes Fig. S1 shows the characterization of peripheral CD8+ T cells from Runx3−/− mice.
T18123 35135-35231 Sentence denotes Fig. S2 shows effector protein expression by Runx3 WT and KO cells at day 4 of in vitro culture.
T18124 35232-35297 Sentence denotes Primer sequences used for ChIP experiments are shown in Table S1.
T18125 35298-35396 Sentence denotes Online supplemental material is available at http://www.jem.org/cgi/content/full/jem.20081242/DC1.
T18355 6546-6745 Sentence denotes Kinetics of gene expression during CD8+ T cell differentiation. (A) Kinetics of Prf1, Gzmb, Tbx21 (T-bet), and Eomes mRNA expression in differentiating P14 CD8+ T cells analyzed by Northern blotting.
T18356 6746-6793 Sentence denotes RNA from day 7 Th1 cells was used as a control.
T18357 6794-7005 Sentence denotes Sizes of mRNA transcripts are indicated. (B) Quantification of relative mRNA amounts by phosphorimager analysis. (C) Kinetics of protein expression in differentiating P14 CD8+ T cells analyzed by immunoblotting.
T18358 7006-7166 Sentence denotes Sizes of protein bands are indicated. (D) Relative protein amounts quantified from the Western blots. (E) Intracellular staining for granzyme B, IFN-γ, and TNF.
T18359 7167-7246 Sentence denotes Granzyme B staining was specific relative to an isotype control (not depicted).
T18360 7247-7470 Sentence denotes Cells were restimulated with PMA and ionomycin for 4 h. (F) FACS-based assay to measure cytolytic activity of P14 CD8+ T cells against EL4 targets loaded with 0 (−) or 1 (+) μM Gp33 peptide (effector-to-target ratio = 5:1).
T18361 7471-7603 Sentence denotes Percentage of Annexin V+ (apoptotic) target cells in the CD8-negative EL4 target population (dot plots) was determined (histograms).
T18362 7604-7759 Sentence denotes Cytolytic activity was blocked by incubation with 2 mM EGTA (not depicted), confirming involvement of the granule exocytosis (perforin–granzyme B) pathway.
T18363 7760-7844 Sentence denotes Data are representative of at least five (A–E) or three (F) independent experiments.
T19200 11884-12062 Sentence denotes Regulation of perforin, granzyme B, and IFN-γ expression by T-bet and Eomes in differentiating CTLs. (A) IFN-γ expression by WT (Tbx21+/+) and T-bet–deficient (Tbx21−/−) T cells.
T19201 12063-12239 Sentence denotes Naive CD8+ T cells, or cells activated and cultured for 4 or 6 d, were restimulated with PMA and ionomycin for 6 h, and IFN-γ expression was assessed by intracellular staining.
T19202 12240-12528 Sentence denotes Numbers show the percentage of IFN-γ+ cells. (B) Northern blot analysis of Prf1 and GzmB mRNA expression in WT or T-bet–deficient CD8+ T cells activated and either left uninfected (uninf) or transduced with retroviruses expressing Eomes-VP16 (Eo-VP16) or an empty IRES-GFP cassette (GFP).
T19203 12529-12581 Sentence denotes Total cellular RNA was analyzed on day 6 of culture.
T19204 12582-12992 Sentence denotes The frequency of transduced cells in the cultures was equivalent for both constructs (∼65–70% GFP+ cells; not depicted). (C) Granzyme B and IFN-γ expression by Tbx21+/+ and Tbx21−/− T cells analyzed in restimulated cells that had been cultured for 5 d. (D) IFN-γ production by cells transduced with Eo-VP16 or control (GFP) retroviruses (RV) measured on day 4 after 6 h of restimulation with PMA and ionomycin.
T19205 12993-13042 Sentence denotes Numbers show the percentage of GFP+ IFN-γ+ cells.
T19206 13043-13130 Sentence denotes Results are representative of three (A and C) or two (B and D) independent experiments.
T20952 19206-19288 Sentence denotes Runx3 controls Eomes, perforin, granzyme B, and IFN-γ expression in effector CTLs.
T20953 19289-19471 Sentence denotes Runx3+/+ or Runx3−/− CD8+ T cells were activated and transduced with retroviruses bearing an empty IRES-GFP cassette (GFP) or also encoding Eomes-VP16 (Eo-VP16) or Myc-Runx3 (Runx3).
T20954 19472-19677 Sentence denotes The frequency of transduced cells in the cultures was equivalent for all constructs (∼75–90% GFP+ cells; not depicted). (A) Protein expression in whole-cell extracts (day 6) was analyzed by immunoblotting.
T20955 19678-20010 Sentence denotes Overexpression of Eomes-VP16 cannot be detected with the Eomes antibody, as the C-terminal epitope is within the region that has been replaced with the VP16 transactivation domain. (B) Expression of granzyme B and IFN-γ after culture for 6 d and restimulation for 4 h with PMA and ionomycin was determined by intracellular staining.
T20956 20011-20189 Sentence denotes The percentage of positively stained cells is shown above the gate; the mean fluorescence intensity (MFI) of granzyme B staining for the total population is shown below the gate.
T20957 20190-20249 Sentence denotes The vertical gray lines indicate the MFI for WT GFP+ cells.
T20958 20250-20405 Sentence denotes Results are representative of at least two independent experiments. (C) Schematic diagram of the transcriptional network involving Runx3 and T-box factors.
T20959 20406-20482 Sentence denotes T-bet is induced by TCR signals and is essential for early IFN-γ expression.
T20960 20483-20604 Sentence denotes Runx3 is present in naive CD8+ T cells and represses Runx1 and induces Eomes, perforin, granzyme B, and IFN-γ expression.
T20961 20605-20750 Sentence denotes Eomes may participate in sustaining late IFN-γ expression, whereas Runx3 and Eomes (but not T-bet) may cooperate to activate perforin expression.
T20962 20751-20867 Sentence denotes The dotted line indicates the partial effect of T-bet deficiency on Gzmb mRNA but not granzyme B protein expression.
T2695 4291-4352 Sentence denotes A cell culture system to monitor effector CTL differentiation
T2696 4353-4477 Sentence denotes We used a simple cell culture system to examine the kinetics of effector gene expression during CD8+ T cell differentiation.
T2697 4478-4713 Sentence denotes Naive CD8+ T cells from P14 TCR transgenic mice were activated for 2 d with anti-CD3 and anti-CD28 or with splenic APCs in the presence of Gp33 peptide, and were cultured in media containing 100 U/ml of recombinant human IL-2 (rhIL-2).
T2698 4714-4994 Sentence denotes We used TCR transgenic mice for these experiments because they provide a reliable source of CD8+ T cells that are truly naive; however, we chose not to stimulate cells with antigen in most experiments so as to avoid contamination with proteins and nucleic acids derived from APCs.
T2699 4995-5216 Sentence denotes There were only minor differences in gene expression during differentiation induced by antigen/APC versus anti-CD3/anti-CD28, and the major conclusions presented in this report are the same for both activating conditions.
T2700 5217-5318 Sentence denotes Under our culture conditions, activated CD8+ T cells expanded exponentially and accumulated for >8 d.
T2701 5319-5467 Sentence denotes We limited our analysis to the first 6–8 d after activation, a period that coincides with clonal expansion of CD8+ T cells after activation in vivo.
T4758 9126-9200 Sentence denotes Distinct kinetics of T-bet and Eomes expression during CTL differentiation
T4759 9201-9404 Sentence denotes The T-box transcription factors T-bet and Eomes have been linked to the regulation of genes encoding effector cytokines (e.g., Ifng) and genes important for cytolytic function (e.g., Prf1 and GzmB) (20).
T4760 9405-9518 Sentence denotes We investigated the kinetics of expression of these transcription factors in our in vitro cultures (Fig. 1, A–D).
T4761 9519-9741 Sentence denotes T-bet mRNA and protein were not detectable in naive CD8+ T cells, but were strongly induced upon TCR priming (day 2) and remained expressed through day 6 of differentiation (Fig. 1, A and C; quantified in Fig. 1, B and D).
T4762 9742-9952 Sentence denotes In contrast, Eomes expression was low or undetectable at both the mRNA and protein levels in naive CD8+ T cells, and TCR priming in culture had only a modest effect on its expression at day 2 (Fig. 1, A and C).
T4763 9953-10051 Sentence denotes Strong induction of Eomes mRNA and protein was only observed at day 4 and later (Fig. 1, A and C).
T4764 10052-10262 Sentence denotes T-bet mRNA expression slightly preceded the expression of GzmB mRNA; similarly, Eomes mRNA and protein were expressed ∼1 d ahead of the reexpression of perforin mRNA and protein, respectively (Fig. 1, B and D).
T4765 10263-10436 Sentence denotes This detailed kinetic analysis suggested that, under our culture conditions, T-bet and Eomes contribute to distinct aspects of gene transcription during CTL differentiation.
T4766 10437-10633 Sentence denotes T-bet is required early for IFN-γ production, and our data suggested that Eomes might not function during this early period but rather might contribute later to the control of perforin expression.
T4767 10634-10883 Sentence denotes Our data seemed most consistent with a model in which TCR signals induce T-bet, which in turn induces IFN-γ (17) and possibly granzyme B; subsequently, Eomes is induced during the period of clonal expansion in IL-2 and activates perforin expression.
T6109 10885-10958 Sentence denotes Perforin and granzyme B expression are not appreciably regulated by T-bet
T6110 10959-11141 Sentence denotes To test the model outlined in the previous paragraph directly, we compared the expression of IFN-γ, perforin, and granzyme B in CD8+ T cells from WT and Tbx21 (T-bet)-deficient mice.
T6111 11142-11241 Sentence denotes As expected (17, 21), naive Tbx21−/− CD8+ T cells produced IFN-γ poorly upon activation (Fig. 2 A).
T6112 11242-11424 Sentence denotes Notably, this deleterious effect of T-bet deficiency was only observed in differentiating CD8+ T cells until day 4 of culture but was almost completely mitigated by day 6 (Fig. 2 A).
T6113 11425-11532 Sentence denotes This most likely reflected compensation by Eomes, which was strongly induced between days 4 and 6 (Fig. 1).
T14376 26310-26356 Sentence denotes Isolation and culture of primary CD8+ T cells.
T14377 26357-26584 Sentence denotes CD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (>95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells.
T14378 26585-26693 Sentence denotes CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec).
T14379 26694-27122 Sentence denotes All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG.
T14380 27123-27276 Sentence denotes After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2.
T14381 27277-27413 Sentence denotes Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2.
T20175 15058-15589 Sentence denotes Key role for Runx3 in effector CTL differentiation. (A) Western analysis of Runx3, Eomes, T-bet, and perforin expression in Runx3+/+ versus Runx3−/− CD8+ SP T cells differentiated for 6 d. β-Actin was used as a loading control. (B) Northern blot analysis of Prf1 mRNA expression in Runx3+/+ versus Runx3−/− CD8+ T cells differentiated for 6 d. β–Actin was used as a loading control. (C) Expression of granzyme B, IFN-γ, TNF, and IL-2 by resting or restimulated (6 h) Runx3+/+ versus Runx3−/− CD8+ SP T cells differentiated for 6 d.
T20176 15590-15660 Sentence denotes The vertical gray line indicates the granzyme B MFI for WT GFP+ cells.
T20177 15661-15804 Sentence denotes Results in A–C are representative of two independent experiments. (D) ChIP analysis of binding of endogenous Runx3 and Eomes to the Prf1 locus.
T20178 15805-15931 Sentence denotes Enrichment of the indicated genomic regions was evaluated by real-time PCR of DNA from immunoprecipitated and input chromatin.
T20179 15932-16063 Sentence denotes The data are the means of duplicate measurements from two chromatin preparations from two independent CD8+ T cell differentiations.
T20180 16064-16186 Sentence denotes The efficiency of recovery of input for the −1-kb region of Prf1 was 0.97% for the Runx3 ChIP and 0.5% for the Eomes ChIP.
T77 0-92 Sentence denotes Runx3 and T-box proteins cooperate to establish the transcriptional program of effector CTLs
T78 104-225 Sentence denotes Activation of naive CD8+ T cells with antigen induces their differentiation into effector cytolytic T lymphocytes (CTLs).
T79 226-390 Sentence denotes CTLs lyse infected or aberrant target cells by exocytosis of lytic granules containing the pore-forming protein perforin and a family of proteases termed granzymes.
T80 391-676 Sentence denotes We show that effector CTL differentiation occurs in two sequential phases in vitro, characterized by early induction of T-bet and late induction of Eomesodermin (Eomes), T-box transcription factors that regulate the early and late phases of interferon (IFN) γ expression, respectively.
T81 677-779 Sentence denotes In addition, we demonstrate a critical role for the transcription factor Runx3 in CTL differentiation.
T82 780-1136 Sentence denotes Runx3 regulates Eomes expression as well as expression of three cardinal markers of the effector CTL program: IFN-γ, perforin, and granzyme B. Our data point to the existence of an elaborate transcriptional network in which Runx3 initially induces and then cooperates with T-box transcription factors to regulate gene transcription in differentiating CTLs.
T7962 13902-14004 Sentence denotes Runx3 controls multiple aspects of the CTL differentiation program, in part through induction of Eomes
T7963 14005-14214 Sentence denotes Because Runx3 is highly expressed in peripheral CD8+ T cells, and because of the T-bet-Runx3 cooperation we observed earlier in CD4+ T cells (15), we examined the role of Runx3 in effector CTL differentiation.
T1 0-92 Sentence denotes Runx3 and T-box proteins cooperate to establish the transcriptional program of effector CTLs
T2 95-103 Sentence denotes Abstract
T3 104-225 Sentence denotes Activation of naive CD8+ T cells with antigen induces their differentiation into effector cytolytic T lymphocytes (CTLs).
T4 226-390 Sentence denotes CTLs lyse infected or aberrant target cells by exocytosis of lytic granules containing the pore-forming protein perforin and a family of proteases termed granzymes.
T5 391-676 Sentence denotes We show that effector CTL differentiation occurs in two sequential phases in vitro, characterized by early induction of T-bet and late induction of Eomesodermin (Eomes), T-box transcription factors that regulate the early and late phases of interferon (IFN) γ expression, respectively.
T6 677-779 Sentence denotes In addition, we demonstrate a critical role for the transcription factor Runx3 in CTL differentiation.
T7 780-922 Sentence denotes Runx3 regulates Eomes expression as well as expression of three cardinal markers of the effector CTL program: IFN-γ, perforin, and granzyme B.
T8 923-1136 Sentence denotes Our data point to the existence of an elaborate transcriptional network in which Runx3 initially induces and then cooperates with T-box transcription factors to regulate gene transcription in differentiating CTLs.
T9 1138-1413 Sentence denotes Naive CD8+ T cells differentiate into effector CTLs with the ability to lyse antigen-bearing target cells by exocytosis of lytic granules containing perforin and granzymes, and to produce inflammatory cytokines such as IFN-γ and TNF upon restimulation through the TCR (1, 2).
T10 1414-1547 Sentence denotes In vivo experiments have elucidated many critical parameters governing the development and evolution of primary CTL responses (3, 4).
T11 1548-1723 Sentence denotes In this study, we have used in vitro systems such as those developed to study CD4+ T cell differentiation to define the molecular basis of effector CTL differentiation (5, 6).
T12 1724-1867 Sentence denotes The T-box transcription factors Eomesodermin (Eomes) and T-bet are needed for important aspects of effector and memory CTL differentiation (7).
T13 1868-2053 Sentence denotes In uninfected mice, compound deletion of the Tbx21 (encoding T-bet) and eomesodermin genes is associated with a selective loss of CD8+ T cells with an IL-2Rβ–high, memory phenotype (8).
T14 2054-2263 Sentence denotes Mice deficient for both T-bet and Eomes in T cells have impaired expression of cytolytic mediators, manifest poor cytolytic activity, and fail to control acute lymphocytic choriomeningitis virus infection (9).
T15 2264-2617 Sentence denotes Nevertheless, the specific roles of T-bet and Eomes in clonal expansion and CTL differentiation have not yet been resolved: in particular, it is not known whether these transcription factors function redundantly to control effector CD8+ T cell differentiation, and whether they do so directly by targeting specific effector cytokine and cytolytic genes.
T16 2618-2761 Sentence denotes Runx proteins, a family of three DNA-binding transcription factors, control thymocyte differentiation and the CD4/CD8 lineage decision (10–13).
T17 2762-2906 Sentence denotes Runx3 and perforin mRNA are expressed by double-positive (DP) thymocytes and CD8+ single-positive (SP) thymocytes but not in CD4+ SP cells (14).
T18 2907-3155 Sentence denotes Although Runx3 is not expressed in naive CD4+ T cells, its expression is up-regulated during Th1 cell differentiation, and Runx3 influences Th1 cell differentiation and function through direct regulation of the Il4 and Ifng cytokine genes (15, 16).
T19 3156-3314 Sentence denotes In contrast, all three Runx proteins are expressed in mature CD8+ T cells (10, 12), and Runx3-deficient CD8+ T cells show reduced cytolytic activity (12, 13).
T20 3315-3393 Sentence denotes We therefore tested whether Runx3 influenced cytolytic T cell differentiation.
T21 3394-3505 Sentence denotes In this report, we show that Runx3 and T-box factors synergistically regulate CTL differentiation and function.
T22 3506-3703 Sentence denotes T-bet is induced quickly upon TCR stimulation and is required for early programming of cytokine production (17), whereas Eomes is induced later during differentiation and sustains IFN-γ expression.
T23 3704-3878 Sentence denotes Runx3 is required for Eomes and perforin expression, and both Eomes and Runx3 bind at the Prf1 locus; in contrast, perforin expression is unaffected in T-bet–deficient cells.
T24 3879-4021 Sentence denotes T cells lacking Runx3 show decreased expression of IFN-γ and granzyme B, and Runx3 also binds the promoter regions of the Ifng and Gzmb genes.
T25 4022-4265 Sentence denotes Collectively, these results provide evidence for a complex transcriptional network in which Runx3 is a primary regulator of Gzmb expression but synergizes with T-bet and Eomes, respectively, to promote transcription of the Ifng and Prf1 genes.
T26 4267-4289 Sentence denotes RESULTS AND DISCUSSION
T27 4291-4352 Sentence denotes A cell culture system to monitor effector CTL differentiation
T28 4353-4477 Sentence denotes We used a simple cell culture system to examine the kinetics of effector gene expression during CD8+ T cell differentiation.
T29 4478-4713 Sentence denotes Naive CD8+ T cells from P14 TCR transgenic mice were activated for 2 d with anti-CD3 and anti-CD28 or with splenic APCs in the presence of Gp33 peptide, and were cultured in media containing 100 U/ml of recombinant human IL-2 (rhIL-2).
T30 4714-4994 Sentence denotes We used TCR transgenic mice for these experiments because they provide a reliable source of CD8+ T cells that are truly naive; however, we chose not to stimulate cells with antigen in most experiments so as to avoid contamination with proteins and nucleic acids derived from APCs.
T31 4995-5216 Sentence denotes There were only minor differences in gene expression during differentiation induced by antigen/APC versus anti-CD3/anti-CD28, and the major conclusions presented in this report are the same for both activating conditions.
T32 5217-5318 Sentence denotes Under our culture conditions, activated CD8+ T cells expanded exponentially and accumulated for >8 d.
T33 5319-5467 Sentence denotes We limited our analysis to the first 6–8 d after activation, a period that coincides with clonal expansion of CD8+ T cells after activation in vivo.
T34 5469-5558 Sentence denotes Distinct expression kinetics of perforin and granzyme B during CTL development in culture
T35 5559-5706 Sentence denotes Our experiments revealed clear differences in the kinetics of perforin, granzyme B, and cytokine expression during CD8+ T cell activation (Fig. 1).
T36 5707-5809 Sentence denotes Naive T cells showed detectable expression of perforin mRNA as well as perforin protein (Fig. 1, A–D).
T37 5810-6037 Sentence denotes Relative to its expression in naive T cells, perforin (Prf1) mRNA expression did not increase appreciably at day 2 but showed a reproducible decrease at day 4, followed by robust reexpression between days 4 and 8 (Fig. 1, A–D).
T38 6038-6325 Sentence denotes In contrast, granzyme B (Gzmb) mRNA was low or undetectable in naive T cells but was strongly up-regulated by day 2 after stimulation and increased progressively until day 6 (Fig. 1, A and B); similarly, granzyme B protein was expressed by day 4 and remained high until day 6 (Fig. 1 E).
T39 6326-6535 Sentence denotes As expected, a small fraction of naive T cells expressed the cytokines IFN-γ and TNF in response to stimulation, and this capacity increased significantly in differentiated cells (Fig. 1 E; see also Fig. 2 A).
T40 6536-6545 Sentence denotes Figure 1.
T41 6546-6745 Sentence denotes Kinetics of gene expression during CD8+ T cell differentiation. (A) Kinetics of Prf1, Gzmb, Tbx21 (T-bet), and Eomes mRNA expression in differentiating P14 CD8+ T cells analyzed by Northern blotting.
T42 6746-6793 Sentence denotes RNA from day 7 Th1 cells was used as a control.
T43 6794-7005 Sentence denotes Sizes of mRNA transcripts are indicated. (B) Quantification of relative mRNA amounts by phosphorimager analysis. (C) Kinetics of protein expression in differentiating P14 CD8+ T cells analyzed by immunoblotting.
T44 7006-7166 Sentence denotes Sizes of protein bands are indicated. (D) Relative protein amounts quantified from the Western blots. (E) Intracellular staining for granzyme B, IFN-γ, and TNF.
T45 7167-7246 Sentence denotes Granzyme B staining was specific relative to an isotype control (not depicted).
T46 7247-7470 Sentence denotes Cells were restimulated with PMA and ionomycin for 4 h. (F) FACS-based assay to measure cytolytic activity of P14 CD8+ T cells against EL4 targets loaded with 0 (−) or 1 (+) μM Gp33 peptide (effector-to-target ratio = 5:1).
T47 7471-7603 Sentence denotes Percentage of Annexin V+ (apoptotic) target cells in the CD8-negative EL4 target population (dot plots) was determined (histograms).
T48 7604-7759 Sentence denotes Cytolytic activity was blocked by incubation with 2 mM EGTA (not depicted), confirming involvement of the granule exocytosis (perforin–granzyme B) pathway.
T49 7760-7978 Sentence denotes Data are representative of at least five (A–E) or three (F) independent experiments.We evaluated antigen-dependent cytolytic function in a short-term assay in which target cell death was measured within 2 h (Fig. 1 F).
T50 7979-8122 Sentence denotes By limiting the duration of TCR stimulation, this strategy minimizes cytolysis secondary to new gene expression during the period of the assay.
T51 8123-8349 Sentence denotes Naive T cells did not display significant cytolytic function in this short-term assay (unpublished data), most likely because they express immature (unprocessed) forms of perforin and lack the capacity to degranulate (18, 19).
T52 8350-8523 Sentence denotes Even after activation for 2 or 4 d, the cells showed poor cytolytic activity (Fig. 1 F), in striking contrast to their capacity for efficient cytokine production (Fig. 1 E).
T53 8524-8679 Sentence denotes Only cells cultured until day 6 displayed robust cytotoxicity, as judged by their ability to induce apoptosis in a large number of target cells (Fig. 1 F).
T54 8680-8993 Sentence denotes These results show that after a strong priming stimulus through TCRs and co-stimulatory receptors in vitro, granzyme B expression and the ability to produce effector cytokines are programmed early, whereas perforin expression and cytolytic function are induced later, during the phase of clonal expansion in IL-2.
T55 8994-9124 Sentence denotes Therefore, the two major effector functions of CTL, cytokine production and cytolytic activity, are not intrinsically coregulated.
T56 9126-9200 Sentence denotes Distinct kinetics of T-bet and Eomes expression during CTL differentiation
T57 9201-9404 Sentence denotes The T-box transcription factors T-bet and Eomes have been linked to the regulation of genes encoding effector cytokines (e.g., Ifng) and genes important for cytolytic function (e.g., Prf1 and GzmB) (20).
T58 9405-9518 Sentence denotes We investigated the kinetics of expression of these transcription factors in our in vitro cultures (Fig. 1, A–D).
T59 9519-9741 Sentence denotes T-bet mRNA and protein were not detectable in naive CD8+ T cells, but were strongly induced upon TCR priming (day 2) and remained expressed through day 6 of differentiation (Fig. 1, A and C; quantified in Fig. 1, B and D).
T60 9742-9952 Sentence denotes In contrast, Eomes expression was low or undetectable at both the mRNA and protein levels in naive CD8+ T cells, and TCR priming in culture had only a modest effect on its expression at day 2 (Fig. 1, A and C).
T61 9953-10051 Sentence denotes Strong induction of Eomes mRNA and protein was only observed at day 4 and later (Fig. 1, A and C).
T62 10052-10262 Sentence denotes T-bet mRNA expression slightly preceded the expression of GzmB mRNA; similarly, Eomes mRNA and protein were expressed ∼1 d ahead of the reexpression of perforin mRNA and protein, respectively (Fig. 1, B and D).
T63 10263-10436 Sentence denotes This detailed kinetic analysis suggested that, under our culture conditions, T-bet and Eomes contribute to distinct aspects of gene transcription during CTL differentiation.
T64 10437-10633 Sentence denotes T-bet is required early for IFN-γ production, and our data suggested that Eomes might not function during this early period but rather might contribute later to the control of perforin expression.
T65 10634-10883 Sentence denotes Our data seemed most consistent with a model in which TCR signals induce T-bet, which in turn induces IFN-γ (17) and possibly granzyme B; subsequently, Eomes is induced during the period of clonal expansion in IL-2 and activates perforin expression.
T66 10885-10958 Sentence denotes Perforin and granzyme B expression are not appreciably regulated by T-bet
T67 10959-11141 Sentence denotes To test the model outlined in the previous paragraph directly, we compared the expression of IFN-γ, perforin, and granzyme B in CD8+ T cells from WT and Tbx21 (T-bet)-deficient mice.
T68 11142-11241 Sentence denotes As expected (17, 21), naive Tbx21−/− CD8+ T cells produced IFN-γ poorly upon activation (Fig. 2 A).
T69 11242-11424 Sentence denotes Notably, this deleterious effect of T-bet deficiency was only observed in differentiating CD8+ T cells until day 4 of culture but was almost completely mitigated by day 6 (Fig. 2 A).
T70 11425-11532 Sentence denotes This most likely reflected compensation by Eomes, which was strongly induced between days 4 and 6 (Fig. 1).
T71 11533-11666 Sentence denotes In contrast, T-bet–deficient T cells cultured for 6 d showed no defect in perforin mRNA expression (Fig. 2 B, compare lanes 1 and 4).
T72 11667-11873 Sentence denotes We consistently observed a modest reduction in GzmB mRNA in T-bet–deficient T cells (Fig. 2 B, compare lanes 1 and 4), which did not translate into a decrease in expression of granzyme B protein (Fig. 2 C).
T73 11874-11883 Sentence denotes Figure 2.
T74 11884-12062 Sentence denotes Regulation of perforin, granzyme B, and IFN-γ expression by T-bet and Eomes in differentiating CTLs. (A) IFN-γ expression by WT (Tbx21+/+) and T-bet–deficient (Tbx21−/−) T cells.
T75 12063-12239 Sentence denotes Naive CD8+ T cells, or cells activated and cultured for 4 or 6 d, were restimulated with PMA and ionomycin for 6 h, and IFN-γ expression was assessed by intracellular staining.
T76 12240-12528 Sentence denotes Numbers show the percentage of IFN-γ+ cells. (B) Northern blot analysis of Prf1 and GzmB mRNA expression in WT or T-bet–deficient CD8+ T cells activated and either left uninfected (uninf) or transduced with retroviruses expressing Eomes-VP16 (Eo-VP16) or an empty IRES-GFP cassette (GFP).
T77 12529-12581 Sentence denotes Total cellular RNA was analyzed on day 6 of culture.
T78 12582-12992 Sentence denotes The frequency of transduced cells in the cultures was equivalent for both constructs (∼65–70% GFP+ cells; not depicted). (C) Granzyme B and IFN-γ expression by Tbx21+/+ and Tbx21−/− T cells analyzed in restimulated cells that had been cultured for 5 d. (D) IFN-γ production by cells transduced with Eo-VP16 or control (GFP) retroviruses (RV) measured on day 4 after 6 h of restimulation with PMA and ionomycin.
T79 12993-13042 Sentence denotes Numbers show the percentage of GFP+ IFN-γ+ cells.
T80 13043-13436 Sentence denotes Results are representative of three (A and C) or two (B and D) independent experiments.To examine the role of Eomes, we transduced naive CD8+ T cells from WT and Tbx21−/− mice with retroviruses containing internal ribosome entry site (IRES)–GFP that were either empty or encoded a strongly transactivating version of Eomes (Eo-VP16) (8), and expanded them for 6 d under our culture conditions.
T81 13437-13587 Sentence denotes Eo-VP16, but not the empty GFP retrovirus, increased perforin expression in both WT and T-bet–deficient CD8+ T cells (Fig. 2 B, lanes 2, 3, 5, and 6).
T82 13588-13711 Sentence denotes As expected, Eo-VP16 also rescued the early defect in IFN-γ production observed in T-bet–deficient CD8+ T cells (Fig. 2 D).
T83 13712-13900 Sentence denotes However, Eo-VP16 did not induce GzmB mRNA expression in either WT or T-bet–deficient cells; thus, the partial T-bet dependence of GzmB mRNA expression cannot be compensated for by Eo-VP16.
T84 13902-14004 Sentence denotes Runx3 controls multiple aspects of the CTL differentiation program, in part through induction of Eomes
T85 14005-14214 Sentence denotes Because Runx3 is highly expressed in peripheral CD8+ T cells, and because of the T-bet-Runx3 cooperation we observed earlier in CD4+ T cells (15), we examined the role of Runx3 in effector CTL differentiation.
T86 14215-14385 Sentence denotes We isolated CD8+ T cells from Runx3−/− (KO) mice of the outbred ICR background and their WT Runx3+/+ littermates by positive selection with anti-CD8 magnetic beads (Figs.
T87 14386-14464 Sentence denotes S1 and S2, available at http://www.jem.org/cgi/content/full/jem.20081242/DC1).
T88 14465-14644 Sentence denotes Strikingly, Runx3−/− CD8+ T cells were strongly impaired in their ability to differentiate into effector CTLs, as judged by expression of perforin, granzyme B, and IFN-γ (Fig. 3).
T89 14645-14796 Sentence denotes Compared with WT T cells, perforin mRNA and protein expression were essentially undetectable in Runx3−/− T cells at day 6 of culture (Fig. 3, A and B).
T90 14797-14911 Sentence denotes Runx3−/− T cells also had no detectable Eomes expression; in contrast, T-bet expression was unimpaired (Fig. 3 A).
T91 14912-15047 Sentence denotes Furthermore, Runx3 was required for maximal production of IFN-γ, but not TNF or IL-2, by CD8+ T cells restimulated at day 6 (Fig. 3 C).
T92 15048-15057 Sentence denotes Figure 3.
T93 15058-15589 Sentence denotes Key role for Runx3 in effector CTL differentiation. (A) Western analysis of Runx3, Eomes, T-bet, and perforin expression in Runx3+/+ versus Runx3−/− CD8+ SP T cells differentiated for 6 d. β-Actin was used as a loading control. (B) Northern blot analysis of Prf1 mRNA expression in Runx3+/+ versus Runx3−/− CD8+ T cells differentiated for 6 d. β–Actin was used as a loading control. (C) Expression of granzyme B, IFN-γ, TNF, and IL-2 by resting or restimulated (6 h) Runx3+/+ versus Runx3−/− CD8+ SP T cells differentiated for 6 d.
T94 15590-15660 Sentence denotes The vertical gray line indicates the granzyme B MFI for WT GFP+ cells.
T95 15661-15804 Sentence denotes Results in A–C are representative of two independent experiments. (D) ChIP analysis of binding of endogenous Runx3 and Eomes to the Prf1 locus.
T96 15805-15931 Sentence denotes Enrichment of the indicated genomic regions was evaluated by real-time PCR of DNA from immunoprecipitated and input chromatin.
T97 15932-16063 Sentence denotes The data are the means of duplicate measurements from two chromatin preparations from two independent CD8+ T cell differentiations.
T98 16064-16479 Sentence denotes The efficiency of recovery of input for the −1-kb region of Prf1 was 0.97% for the Runx3 ChIP and 0.5% for the Eomes ChIP.We previously reported that Th1 cell differentiation was regulated through a feed-forward loop in which T-bet is up-regulated early and induces Runx3, after which T-bet and Runx3 cooperate to induce IFN-γ and silence IL-4, thus promoting stable differentiation toward the Th1 lineage (15, 22).
T99 16480-16950 Sentence denotes Because (a) Runx3 appeared necessary for Eomes induction (Fig. 3 A), (b) the kinetics of Eomes expression paralleled those of perforin expression (Fig. 2), and (c) overexpression of Eo-VP16 in either WT or T-bet–deficient T cells led to an increase in both perforin and IFN-γ expression (Fig. 2, B and D), we asked whether CTL differentiation was also potentially regulated by a feed-forward loop involving these same two classes of Runx and T-box transcription factors.
T100 16951-17143 Sentence denotes Specifically, we asked whether Runx3, which was necessary for Eomes induction, then cooperated with Eomes to regulate transcription of the effector CTL markers perforin, IFN-γ, and granzyme B.
T101 17144-17322 Sentence denotes To test this hypothesis, we used chromatin immunoprecipitation (ChIP) assays to ask whether Eomes and Runx3 bound regulatory regions of the Prf1, Ifng, and Gzmb genes (Fig. 3 D).
T102 17323-17400 Sentence denotes Both proteins associated with gene regulatory regions in differentiated CTLs.
T103 17401-17799 Sentence denotes Runx3 bound to the Prf1 and Gzmb transcription start sites (TSS); to a known IL-2 responsive enhancer located near −1 kb of the Prf1 gene (23); to the distal CTL-specific DNase I hypersensitive site 9 in the Prf1 locus (24); to the Ifng promoter near the TSS, as previously reported for Th1 cells (10); and to several DNase I hypersensitive sites in the Ifng locus (Fig. 3 D and not depicted) (25).
T104 17800-18118 Sentence denotes Eomes bound primarily to the Prf1 TSS and the −1 kb enhancer; this binding was substantially greater than that observed at the promoter of the Il2rb gene, a known direct target of Eomes (8), and comparable to that observed at the Ifng TSS, a known target of T-box proteins in both Th1 and CD8+ T cells (Fig. 3 D) (17).
T105 18119-18305 Sentence denotes To determine whether Runx3 controlled the expression of CTL effector genes through its induction of Eomes, we retrovirally expressed Runx3 and Eo-VP16 in CD8+ T cells from Runx3−/− mice.
T106 18306-18588 Sentence denotes Because of the limited number of CD8+ T cells in these mice, and because we saw no difference between Runx3−/− CD8+CD4− SP and CD8+CD4+ DP cells in our previous experiments, we used total Runx3−/− CD8+ T cells without further fractionation as recipients for retroviral transduction.
T107 18589-18730 Sentence denotes Reconstitution of Runx3−/− CD8+ T cells with Runx3 restored expression of Eomes as well as perforin, granzyme B, and IFN-γ (Fig. 4, A and B).
T108 18731-18917 Sentence denotes In addition, Runx3−/− T cells showed a compensatory up-regulation of Runx1, which was suppressed upon reconstitution with Runx3, indicating that Runx1 is a target of repression by Runx3.
T109 18918-19114 Sentence denotes Notably, Eo-VP16 did not up-regulate perforin expression when expressed in Runx3−/− cells, even though it restored the capacity to induce IFN-γ expression upon TCR restimulation (Fig. 4, A and B).
T110 19115-19195 Sentence denotes This result suggests strongly that perforin expression requires Runx3 and Eomes.
T111 19196-19205 Sentence denotes Figure 4.
T112 19206-19288 Sentence denotes Runx3 controls Eomes, perforin, granzyme B, and IFN-γ expression in effector CTLs.
T113 19289-19471 Sentence denotes Runx3+/+ or Runx3−/− CD8+ T cells were activated and transduced with retroviruses bearing an empty IRES-GFP cassette (GFP) or also encoding Eomes-VP16 (Eo-VP16) or Myc-Runx3 (Runx3).
T114 19472-19677 Sentence denotes The frequency of transduced cells in the cultures was equivalent for all constructs (∼75–90% GFP+ cells; not depicted). (A) Protein expression in whole-cell extracts (day 6) was analyzed by immunoblotting.
T115 19678-20010 Sentence denotes Overexpression of Eomes-VP16 cannot be detected with the Eomes antibody, as the C-terminal epitope is within the region that has been replaced with the VP16 transactivation domain. (B) Expression of granzyme B and IFN-γ after culture for 6 d and restimulation for 4 h with PMA and ionomycin was determined by intracellular staining.
T116 20011-20189 Sentence denotes The percentage of positively stained cells is shown above the gate; the mean fluorescence intensity (MFI) of granzyme B staining for the total population is shown below the gate.
T117 20190-20249 Sentence denotes The vertical gray lines indicate the MFI for WT GFP+ cells.
T118 20250-20405 Sentence denotes Results are representative of at least two independent experiments. (C) Schematic diagram of the transcriptional network involving Runx3 and T-box factors.
T119 20406-20482 Sentence denotes T-bet is induced by TCR signals and is essential for early IFN-γ expression.
T120 20483-20604 Sentence denotes Runx3 is present in naive CD8+ T cells and represses Runx1 and induces Eomes, perforin, granzyme B, and IFN-γ expression.
T121 20605-20750 Sentence denotes Eomes may participate in sustaining late IFN-γ expression, whereas Runx3 and Eomes (but not T-bet) may cooperate to activate perforin expression.
T122 20751-21030 Sentence denotes The dotted line indicates the partial effect of T-bet deficiency on Gzmb mRNA but not granzyme B protein expression.As expected from their defect in perforin and granzyme B expression, Runx3−/− CD8+ T cells showed defective cytolytic activity in a mixed lymphocyte reaction (12).
T123 21031-21159 Sentence denotes However, TCR-stimulated Runx3−/− CD8+ cells were as effective as WT cells in killing tumor cells in a redirected CTL assay (12).
T124 21160-21301 Sentence denotes Furthermore, CD8+ cells from the peritoneal cavity of Runx3−/− mice immunized with certain tumor cells effectively killed these targets (13).
T125 21302-21577 Sentence denotes Therefore, although activation of the perforin/granzyme B machinery is defective in Runx3−/− CD8+ cells, these cells are not entirely devoid of cytolytic activity and could still effectively kill targets, possibly by alternative mechanisms such as the Fas–Fas ligand pathway.
T126 21579-21685 Sentence denotes Runx3 and T-box factors control a complex program of transcriptional regulation during CTL differentiation
T127 21686-21864 Sentence denotes Collectively, these data provide evidence that Runx3, together with T-box factors, orchestrates a complex program of transcriptional regulation in differentiating CTL (Fig. 4 C).
T128 21865-21927 Sentence denotes Runx3 is present in naive CD8+ T cells before activation (12).
T129 21928-22030 Sentence denotes It represses Runx1 and has a positive role in the induction of Eomes, granzyme B, perforin, and IFN-γ.
T130 22031-22161 Sentence denotes Runx3 binds to promoters and putative regulatory regions of the latter three genes, suggesting a direct effect on gene expression.
T131 22162-22271 Sentence denotes Additional experiments are needed to determine whether Eomes and Runx1 are also direct target genes of Runx3.
T132 22272-22373 Sentence denotes Surprisingly, Runx3 contributed to the optimal expression of TNF, IL-2, and IFN-γ at day 4 (Fig. S2).
T133 22374-22541 Sentence denotes For TNF and IL-2, the requirement for Runx3 subsides by day 6 (Fig. 3 C), possibly because of compensation by Runx1, which is derepressed in Runx3−/− cells (Fig. 4 A).
T134 22542-22685 Sentence denotes Runx3 continues to be required for IFN-γ expression even at day 6, perhaps because of its role in the induction of Eomes expression (Fig. 4 A).
T135 22686-22955 Sentence denotes An unexpected finding was that the two T-box transcription factors, T-bet and Eomes, are up-regulated with very different kinetics in CD8+ T cells under our culture conditions and have nonredundant roles in the subsequent expression of key effector proteins (Fig. 4 C).
T136 22956-23116 Sentence denotes T-bet is needed early to confer on activated CD8+ T cells the competence to produce IFN-γ upon restimulation, but its function is less important at later times.
T137 23117-23274 Sentence denotes Eomes, which is induced late and functions downstream of Runx3, may substitute for T-bet in promoting the acute expression of IFN-γ in restimulated CTLs (8).
T138 23275-23531 Sentence denotes Indeed, T-bet and Eomes both contribute to perforin expression in NK cells (8, 26), and Eomes induces granzyme B as effectively as T-bet in developing Th2 cells (7); thus, the relative roles of these T-box transcription factors vary depending on cell type.
T139 23532-23680 Sentence denotes Surprisingly, however, Eomes and T-bet appeared nonredundant in their ability to induce two other markers of CTL function, Prf1 and Gzmb (Fig. 4 C).
T140 23681-23912 Sentence denotes Rather, T-bet and Eomes were involved in regulating granzyme B and perforin expression, respectively: up-regulation of T-bet and Eomes mRNA and protein closely preceded up-regulation of Gzmb and Prf1 mRNA and protein, respectively.
T141 23913-24078 Sentence denotes T-bet had no role in perforin expression under our culture conditions, and Eo-VP16 did not affect granzyme B expression when expressed in T-bet−/− or Runx3−/− cells.
T142 24079-24438 Sentence denotes Because conventional Eomes-deficient mice die before precursor cells can be isolated for bone marrow transfers (27) and because T cells conditionally deficient in Eomes have only recently been described (9), we were unable to introduce Runx3 into Eomes-deficient CD8+ T cells to test formally whether Eomes cooperated with Runx3 to induce perforin expression.
T143 24439-24677 Sentence denotes Collectively, our data are consistent with a transcriptional network in which preexisting Runx3 cooperates with the induced T-box factors T-bet and Eomes and IL-2Rβ signals (unpublished data) to orchestrate CTL differentiation (Fig. 4 C).
T144 24678-25062 Sentence denotes Our data recall the “feed-forward” interaction between T-bet and Runx3 that we previously described in CD4+ (Th1) T cells (15) but are distinct in two respects: in differentiating Th1 cells, T-bet is induced by TCR signals and IFN-γ, and in turn induces Runx3 (15), whereas in differentiating CD8+ T cells, preexisting Runx3 is required to induce the T-box transcription factor Eomes.
T145 25063-25275 Sentence denotes Whole-genome experiments in these and other systems will be required to establish whether cooperation between T-box and Runx family transcription factors is a general feature of cellular differentiation programs.
T146 25277-25298 Sentence denotes MATERIALS AND METHODS
T147 25300-25324 Sentence denotes Antibodies and reagents.
T148 25325-25508 Sentence denotes The following antibodies used for intracellular or surface stains were obtained from eBioscience: anti–IL-2, anti–IFN-γ, anti-TNF, anti–granzyme B, anti-CD8, anti-CD25, and anti-CD44.
T149 25509-25541 Sentence denotes Anti-CD69 was purchased from BD.
T150 25542-25678 Sentence denotes For ChIP experiments, the anti-Eomes antibody was obtained from Abcam and the anti-Runx3 antibody was produced by the Groner laboratory.
T151 25679-25825 Sentence denotes The following antibodies were used for immunoblotting: antiperforin (Abcam), anti-Eomes (Abcam), and anti–Pol-II (Santa Cruz Biotechnology, Inc.).
T152 25826-25863 Sentence denotes The T-bet antibody was provided by L.
T153 25864-25919 Sentence denotes Glimcher (Harvard School of Public Health, Boston, MA).
T154 25920-25998 Sentence denotes The following reagents were used for the experiments presented in this report:
T155 25999-26173 Sentence denotes Annexin V–FITC Apoptosis Detection Kit (BD), CD8 Negative Isolation Kit (Invitrogen), CD8 MicroBeads (Miltenyi Biotec), and SYBR Green PCR Core Reagents (Applied Biosystems).
T156 26174-26308 Sentence denotes The Gp33 peptide (KAVYNFATC) was synthesized by the Tufts University Core Facility, and 10 mM of stock solutions was prepared in DMSO.
T157 26310-26356 Sentence denotes Isolation and culture of primary CD8+ T cells.
T158 26357-26584 Sentence denotes CD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (>95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells.
T159 26585-26693 Sentence denotes CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec).
T160 26694-26899 Sentence denotes All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees.
T161 26900-27122 Sentence denotes For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG.
T162 27123-27276 Sentence denotes After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2.
T163 27277-27413 Sentence denotes Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2.
T164 27415-27460 Sentence denotes Isolation of CD8+ T cells from Runx3−/− mice.
T165 27461-27544 Sentence denotes Runx3-deficient T cells fail to silence CD4 expression normally (Fig. S1) (12, 13).
T166 27545-27717 Sentence denotes We therefore further fractionated the positively selected CD8+ T cells from Runx3 KO mice into CD8+CD4− SP or CD8+CD4+ DP cells by separation using anti-CD4 magnetic beads.
T167 27718-27877 Sentence denotes This yielded a Runx3 KO SP “enriched” population that contained 75% CD8+CD4− cells and a KO DP enriched population that contained 85% CD8+CD4+ cells (Fig. S1).
T168 27878-28033 Sentence denotes The cells were stimulated with anti-CD3+ anti-CD28 for 2 d before removing them from the TCR stimulus and culturing them in media containing 100 U/ml IL-2.
T169 28034-28182 Sentence denotes As previously reported, TCR-induced proliferation of Runx3−/− CD8+ T cells was severely impaired, irrespective of CD4 expression (Fig. S1) (12, 13).
T170 28183-28333 Sentence denotes However, the Runx3−/− cells showed cell-surface expression patterns indicative of activated cells, including up-regulation of CD25 and CD69 (Fig. S1).
T171 28334-28570 Sentence denotes As expected from their ability to up-regulate CD25, Runx3−/− CD8+ T cells responded to IL-2 supplementation after day 2 and efficiently expanded until day 6 of the culture period, albeit at slower rates compared with WT cells (Fig. S1).
T172 28571-29023 Sentence denotes Although a fraction of the KO DP cells silenced CD4 expression after activation, the ratio of SP/DP cells in each enriched population remained constant thereafter, and we did not observe any major differences between these two populations throughout the culture period, indicating that in terms of effector CTL differentiation and under our culture conditions, Runx3−/− CD8+ T cells that also coexpress CD4 are indistinguishable from those that do not.
T173 29024-29110 Sentence denotes The data presented in Fig. S2 are from Runx3 KO SP cells, whereas those shown in Figs.
T174 29111-29153 Sentence denotes 3 and 4 are from total Runx3 KO CD8 cells.
T175 29155-29185 Sentence denotes FACS-based cytotoxicity assay.
T176 29186-29421 Sentence denotes To measure cytotoxicity, EL4 thymoma target cells were loaded with 0 or 1 μM Gp33 peptide for 2 h before a 2-h coincubation with P14 CD8+ T cells at the effector-to-target ratios indicated in the figures in 96-well round-bottom plates.
T177 29422-29521 Sentence denotes After the coincubation period, cells were stained with Annexin V–FITC and anti-CD8–allophycocyanin.
T178 29522-29702 Sentence denotes Data analysis was performed with FlowJo software (Tree Star, Inc.); EL4 target cells (CD8-negative events) were gated, and the percentage of Annexin V+ target cells was determined.
T179 29704-29741 Sentence denotes Cytokine and surface marker staining.
T180 29742-29960 Sentence denotes To assess cytokine production, cells were restimulated with 10 nM PMA + 1 μM ionomycin for 6 h (unless indicated otherwise in the figures), and intracellular cytokine stains were performed as previously described (28).
T181 29961-30351 Sentence denotes To detect expression of surface molecules, cells were washed in PBS, resuspended in FACS wash buffer (3% FBS, 0.1% sodium azide, 30 mM Hepes, 1× PBS) containing the antibodies indicated in the figures at previously optimized concentrations, incubated for 15 min at room temperature (RT), washed, and resuspended in 2% formaldehyde fixative solution before acquisition on a FACSCalibur (BD).
T182 30353-30401 Sentence denotes Retroviral transduction of primary CD8+ T cells.
T183 30402-30574 Sentence denotes For transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g.
T184 30575-30805 Sentence denotes At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus.
T185 30806-30904 Sentence denotes The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h.
T186 30905-30996 Sentence denotes Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L.
T187 30997-31055 Sentence denotes Reiner (University of Pennsylvania, Philadelphia, PA) (8).
T188 31057-31089 Sentence denotes ChIP and real-time PCR analysis.
T189 31090-31336 Sentence denotes 20 × 106 CD8+ T cells per immunoprecipitation were fixed by adding a 1/10th volume of fixation solution (11.1% formaldehyde, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 50 mM Hepes) to 1 volume of culture media and were incubated for 10 or 30 min at RT.
T190 31337-31395 Sentence denotes Fixation was stopped with 120 mM glycine on ice for 5 min.
T191 31396-31615 Sentence denotes Fixed cells were washed 2× with cold PBS, 1× with cold solution I (10 mM Tris [pH 7.5], 10 mM EDTA, 0.5 mM EGTA, 1% Triton X-100), and 1× with cold solution II (10 mM Tris [pH 7.5], 1 mM EDTA, 0.5 mM EGTA, 200 mM NaCl).
T192 31616-31900 Sentence denotes After washes, cell pellets were resuspended at 40 × 106 cells/ml in ChIP lysis buffer (150 mM NaCl, 25 mM Tris [pH 7.5], 1% Triton X-100, 0.1% SDS, 0.5% deoxycholate plus protease and phosphatase inhibitors), and chromatin was sheared with a sonicator to yield 0.5–1-kb DNA fragments.
T193 31901-32110 Sentence denotes After preclearing the sheared chromatin with protein A–sepharose beads and removing 5% as input chromatin, immunoprecipitation was performed by adding optimized antibody amounts (per 20 × 106 cell equivalents:
T194 32111-32278 Sentence denotes 2.5 μg anti-Eomes, 1:100 dilution anti-Runx3), followed by overnight incubation at 4°C; protein A–sepharose beads were added for the last 3 h of the incubation period.
T195 32279-32513 Sentence denotes Beads were washed 2× with RIPA buffer (50 mM Tris [pH 8], 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% deoxycholate), 1× with high salt buffer (50 mM Tris [pH 8], 500 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS), and 1× with TE buffer.
T196 32514-32618 Sentence denotes After the last wash, DNA was eluted by resuspending the beads in elution buffer (1% SDS, 100 mM NaHCO3).
T197 32619-32824 Sentence denotes Both input and ChIP chromatin were then treated with RNase A (5 μg total) for 1 h at 37°C, followed by the addition of proteinase K (100 μg total) and overnight incubation at 65°C to reverse cross-linking.
T198 32825-32977 Sentence denotes DNA was then purified with QIAquick columns (Gel Extraction Kit; QIAGEN) according to the manufacturer's instructions and resuspended in a 50-μl volume.
T199 32978-33218 Sentence denotes For real-time PCR detection of immunoprecipitated targets using the SYBR Green PCR Kit, a standard curve was obtained with serial dilutions of input DNA for each sample, and 1 μl ChIP DNA was used per PCR reaction (performed in duplicates).
T200 33219-33315 Sentence denotes Melt curves and agarose gels were analyzed to ensure amplification of specific target sequences.
T201 33316-33428 Sentence denotes Refer to Table S1 (available at http://www.jem.org/cgi/content/full/jem.20081242/DC1) for a list of primer sets.
T202 33429-33611 Sentence denotes The data are presented as the number of immunoprecipitated target sequences relative to input chromatin, assuming two copies of target sequence per cell equivalent used for the ChIP.
T203 33613-33648 Sentence denotes Northern and Western blot analyses.
T204 33649-33733 Sentence denotes RNA isolation and Northern blot analysis was performed as previously described (29).
T205 33734-33955 Sentence denotes In brief, 10 μg of total RNA was loaded per lane and transferred to positively charged nylon membranes (Hybond-N+; GE Healthcare), which was confirmed by ethidium bromide staining of ribosomal RNA species on the membrane.
T206 33956-34120 Sentence denotes Membranes were hybridized with 1 ng/ml α-[32P]dCTP–labeled trichloroacetic acid precipitable probe in ExpressHyb hybridization buffer (Clontech Laboratories, Inc.).
T207 34121-34260 Sentence denotes All cDNA probes were confirmed to have the appropriate single-copy specificity under these conditions using genomic Southern blot analysis.
T208 34261-34320 Sentence denotes Band intensities were acquired by phosphorimaging analysis.
T209 34321-34694 Sentence denotes For Western analysis, whole-cell protein lysates were obtained from CD8+ T cells at the time points indicated in the figures during clonal expansion in 100 U/ml IL-2 with lysis buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 10% glycerol, 5 mM EDTA, 1% NP-40) by resuspending samples in 10 μl per 106 cells and incubating on ice for 30 min in the presence of protease inhibitors.
T210 34695-34837 Sentence denotes Immunoblot analysis was performed with the antibodies indicated in the figures after SDS-PAGE (10–30 μg of total protein was loaded per well).
T211 34838-35021 Sentence denotes Quantification of detected protein was performed with an Intelligent Dark Box unit (LAS-3000; Fujifilm) and normalized for loading with the amount of RNA Pol-II detected in each lane.
T212 35023-35052 Sentence denotes Online supplemental material.
T213 35053-35134 Sentence denotes Fig. S1 shows the characterization of peripheral CD8+ T cells from Runx3−/− mice.
T214 35135-35231 Sentence denotes Fig. S2 shows effector protein expression by Runx3 WT and KO cells at day 4 of in vitro culture.
T215 35232-35297 Sentence denotes Primer sequences used for ChIP experiments are shown in Table S1.
T216 35298-35396 Sentence denotes Online supplemental material is available at http://www.jem.org/cgi/content/full/jem.20081242/DC1.
T217 35398-35420 Sentence denotes Supplementary Material
T218 35421-35450 Sentence denotes [Supplemental Material Index]

ICD10

Id Subject Object Predicate Lexical cue
T2072 2214-2242 http://purl.bioontology.org/ontology/ICD10/A87.2 denotes lymphocytic choriomeningitis

events-check-again

Id Subject Object Predicate Lexical cue Negation Speculation
T2557 1144-1147 Protein denotes CD8
T2558 1287-1295 Protein denotes perforin
T2559 1318-1325 Gene_expression denotes produce
T2560 1318-1325 Gene_expression denotes produce
T2561 1357-1362 Protein denotes IFN-γ
T2562 1367-1370 Protein denotes TNF
T2563 1626-1629 Protein denotes CD4
T2564 1756-1768 Protein denotes Eomesodermin
T2565 1770-1775 Protein denotes Eomes
T2566 1781-1786 Protein denotes T-bet
T2567 1897-1905 Negative_regulation denotes deletion
T2568 1897-1905 Negative_regulation denotes deletion
T2569 1913-1918 Protein denotes Tbx21
T2570 1929-1934 Protein denotes T-bet
T2571 1940-1952 Protein denotes eomesodermin
T2572 1998-2001 Protein denotes CD8
T2573 2019-2025 Protein denotes IL-2Rβ
T2574 2059-2068 Negative_regulation denotes deficient
T2575 2059-2068 Negative_regulation denotes deficient
T2576 2078-2083 Protein denotes T-bet
T2577 2088-2093 Protein denotes Eomes
T2578 2300-2305 Protein denotes T-bet
T2579 2310-2315 Protein denotes Eomes
T2580 2496-2499 Protein denotes CD8
T2581 2560-2569 Binding denotes targeting
T2582 2560-2569 Binding denotes targeting
T2583 2728-2731 Protein denotes CD4
T2584 2732-2735 Protein denotes CD8
T2585 2762-2767 Protein denotes Runx3
T2586 2772-2780 Protein denotes perforin
T2587 2790-2799 Transcription denotes expressed true
T2588 2790-2799 Transcription denotes expressed true
T2589 2839-2842 Protein denotes CD8
T2590 2887-2890 Protein denotes CD4
T2591 2916-2921 Protein denotes Runx3
T2592 2929-2938 Gene_expression denotes expressed true
T2593 2948-2951 Protein denotes CD4
T2594 2966-2976 Gene_expression denotes expression
T2595 2980-2992 Positive_regulation denotes up-regulated
T2596 3030-3035 Protein denotes Runx3
T2597 3100-3110 Regulation denotes regulation
T2598 3100-3110 Regulation denotes regulation
T2599 3118-3121 Protein denotes Il4
T2600 3126-3130 Protein denotes Ifng
T2601 3217-3220 Protein denotes CD8
T2602 3244-3249 Protein denotes Runx3
T2603 3250-3259 Negative_regulation denotes deficient
T2604 3260-3263 Protein denotes CD8
T2605 3343-3348 Protein denotes Runx3
T2606 3423-3428 Protein denotes Runx3
T2607 3506-3511 Protein denotes T-bet
T2608 3515-3522 Positive_regulation denotes induced
T2609 3627-3632 Protein denotes Eomes
T2610 3636-3643 Positive_regulation denotes induced
T2611 3677-3685 Positive_regulation denotes sustains
T2612 3686-3691 Protein denotes IFN-γ
T2613 3692-3702 Gene_expression denotes expression
T2614 3704-3709 Protein denotes Runx3
T2615 3713-3721 Positive_regulation denotes required
T2616 3713-3721 Positive_regulation denotes required
T2617 3726-3731 Protein denotes Eomes
T2618 3736-3744 Protein denotes perforin
T2619 3745-3755 Gene_expression denotes expression
T2620 3745-3755 Gene_expression denotes expression
T2621 3766-3771 Protein denotes Eomes
T2622 3776-3781 Protein denotes Runx3
T2623 3782-3786 Binding denotes bind
T2624 3782-3786 Binding denotes bind
T2625 3794-3798 Protein denotes Prf1
T2626 3819-3827 Protein denotes perforin
T2627 3828-3838 Gene_expression denotes expression
T2628 3842-3852 Regulation denotes unaffected true
T2629 3856-3861 Protein denotes T-bet
T2630 3862-3871 Negative_regulation denotes deficient
T2631 3887-3894 Negative_regulation denotes lacking
T2632 3895-3900 Protein denotes Runx3
T2633 3906-3915 Negative_regulation denotes decreased
T2634 3906-3915 Negative_regulation denotes decreased
T2635 3916-3926 Gene_expression denotes expression
T2636 3916-3926 Gene_expression denotes expression
T2637 3930-3935 Protein denotes IFN-γ
T2638 3940-3950 Protein denotes granzyme B
T2639 3956-3961 Protein denotes Runx3
T2640 3967-3972 Binding denotes binds
T2641 3967-3972 Binding denotes binds
T2642 3977-3993 Entity denotes promoter regions
T2643 4001-4005 Protein denotes Ifng
T2644 4010-4014 Protein denotes Gzmb
T2645 4114-4119 Protein denotes Runx3
T2646 4133-4142 Regulation denotes regulator
T2647 4146-4150 Protein denotes Gzmb
T2648 4151-4161 Gene_expression denotes expression
T2649 4182-4187 Protein denotes T-bet
T2650 4192-4197 Protein denotes Eomes
T2651 4216-4223 Positive_regulation denotes promote
T2652 4216-4223 Positive_regulation denotes promote
T2653 4224-4237 Transcription denotes transcription
T2654 4224-4237 Transcription denotes transcription
T2655 4245-4249 Protein denotes Ifng
T2656 4254-4258 Protein denotes Prf1
T3205 4449-4452 Protein denotes CD8
T3206 4484-4487 Protein denotes CD8
T3207 4559-4562 Protein denotes CD3
T3208 4572-4576 Protein denotes CD28
T3209 4699-4703 Protein denotes IL-2
T3210 4705-4711 Protein denotes rhIL-2
T3211 4806-4809 Protein denotes CD8
T3212 5106-5109 Protein denotes CD3
T3213 5115-5119 Protein denotes CD28
T3214 5257-5260 Protein denotes CD8
T3215 5429-5432 Protein denotes CD8
T4491 5478-5488 Gene_expression denotes expression true
T4492 5478-5488 Gene_expression denotes expression true
T4493 5501-5509 Protein denotes perforin
T4494 5514-5524 Protein denotes granzyme B
T4495 5621-5629 Protein denotes perforin
T4496 5631-5641 Protein denotes granzyme B
T4497 5656-5666 Gene_expression denotes expression
T4498 5656-5666 Gene_expression denotes expression
T4499 5674-5677 Protein denotes CD8
T4500 5739-5749 Gene_expression denotes expression
T4501 5739-5749 Gene_expression denotes expression
T4502 5753-5761 Protein denotes perforin
T4503 5778-5786 Protein denotes perforin
T4504 5826-5836 Gene_expression denotes expression
T4505 5855-5863 Protein denotes perforin
T4506 5865-5869 Protein denotes Prf1
T4507 5871-5886 Transcription denotes mRNA expression
T4508 5895-5903 Positive_regulation denotes increase true
T4509 5951-5959 Negative_regulation denotes decrease
T4510 5989-6001 Positive_regulation denotes reexpression
T4511 6051-6061 Protein denotes granzyme B
T4512 6063-6067 Protein denotes Gzmb
T4513 6078-6097 Transcription denotes low or undetectable true
T4514 6132-6144 Positive_regulation denotes up-regulated
T4515 6176-6185 Positive_regulation denotes increased
T4516 6242-6252 Protein denotes granzyme B
T4517 6288-6301 Positive_regulation denotes remained high
T4518 6373-6382 Gene_expression denotes expressed
T4519 6373-6382 Gene_expression denotes expressed
T4520 6397-6402 Protein denotes IFN-γ
T4521 6407-6410 Protein denotes TNF
T4522 6457-6466 Positive_regulation denotes increased
T4523 6457-6466 Positive_regulation denotes increased
T4524 8254-8261 Gene_expression denotes express
T4525 8294-8302 Protein denotes perforin
T4526 8788-8798 Protein denotes granzyme B
T4527 8799-8809 Gene_expression denotes expression
T4528 8886-8894 Protein denotes perforin
T4529 8895-8905 Gene_expression denotes expression
T4530 8933-8940 Positive_regulation denotes induced
T4531 8988-8992 Protein denotes IL-2
T5801 9147-9152 Protein denotes T-bet
T5802 9157-9162 Protein denotes Eomes
T5803 9163-9173 Gene_expression denotes expression
T5804 9163-9173 Gene_expression denotes expression
T5805 9233-9238 Protein denotes T-bet
T5806 9243-9248 Protein denotes Eomes
T5807 9259-9265 Regulation denotes linked
T5808 9259-9265 Regulation denotes linked
T5809 9259-9265 Regulation denotes linked
T5810 9259-9265 Regulation denotes linked
T5811 9259-9265 Regulation denotes linked
T5812 9259-9265 Regulation denotes linked
T5813 9273-9283 Regulation denotes regulation
T5814 9273-9283 Regulation denotes regulation
T5815 9273-9283 Regulation denotes regulation
T5816 9328-9332 Protein denotes Ifng
T5817 9384-9388 Protein denotes Prf1
T5818 9393-9397 Protein denotes GzmB
T5819 9437-9447 Gene_expression denotes expression true
T5820 9437-9447 Gene_expression denotes expression true
T5821 9519-9524 Protein denotes T-bet
T5822 9551-9561 Gene_expression denotes detectable true
T5823 9571-9574 Protein denotes CD8
T5824 9603-9610 Positive_regulation denotes induced
T5825 9640-9648 Positive_regulation denotes remained
T5826 9649-9658 Gene_expression denotes expressed
T5827 9755-9760 Protein denotes Eomes
T5828 9761-9771 Gene_expression denotes expression
T5829 9776-9795 Positive_regulation denotes low or undetectable true
T5830 9841-9844 Protein denotes CD8
T5831 9900-9906 Regulation denotes effect true
T5832 9914-9924 Gene_expression denotes expression
T5833 9960-9969 Positive_regulation denotes induction
T5834 9973-9978 Protein denotes Eomes
T5835 10052-10057 Protein denotes T-bet
T5836 10058-10073 Transcription denotes mRNA expression
T5837 10096-10106 Gene_expression denotes expression
T5838 10110-10114 Protein denotes GzmB
T5839 10132-10137 Protein denotes Eomes
T5840 10160-10169 Gene_expression denotes expressed
T5841 10188-10200 Gene_expression denotes reexpression
T5842 10204-10212 Protein denotes perforin
T5843 10340-10345 Protein denotes T-bet
T5844 10350-10355 Protein denotes Eomes
T5845 10437-10442 Protein denotes T-bet
T5846 10446-10454 Positive_regulation denotes required
T5847 10465-10470 Protein denotes IFN-γ
T5848 10471-10481 Gene_expression denotes production
T5849 10511-10516 Protein denotes Eomes
T5850 10578-10588 Positive_regulation denotes contribute true
T5851 10602-10609 Regulation denotes control
T5852 10613-10621 Protein denotes perforin
T5853 10622-10632 Gene_expression denotes expression
T5854 10700-10706 Positive_regulation denotes induce
T5855 10707-10712 Protein denotes T-bet
T5856 10728-10735 Positive_regulation denotes induces
T5857 10728-10735 Positive_regulation denotes induces
T5858 10736-10741 Protein denotes IFN-γ
T5859 10760-10770 Protein denotes granzyme B
T5860 10786-10791 Protein denotes Eomes
T5861 10795-10802 Positive_regulation denotes induced
T5862 10844-10848 Protein denotes IL-2
T5863 10853-10862 Positive_regulation denotes activates
T5864 10863-10871 Protein denotes perforin
T5865 10872-10882 Gene_expression denotes expression
T7247 10885-10893 Protein denotes Perforin
T7248 10898-10908 Protein denotes granzyme B
T7249 10909-10919 Gene_expression denotes expression
T7250 10909-10919 Gene_expression denotes expression
T7251 10940-10949 Regulation denotes regulated true
T7252 10940-10949 Regulation denotes regulated true
T7253 10953-10958 Protein denotes T-bet
T7254 11038-11048 Gene_expression denotes expression true
T7255 11038-11048 Gene_expression denotes expression true
T7256 11038-11048 Gene_expression denotes expression true
T7257 11052-11057 Protein denotes IFN-γ
T7258 11059-11067 Protein denotes perforin
T7259 11073-11083 Protein denotes granzyme B
T7260 11087-11090 Protein denotes CD8
T7261 11112-11117 Protein denotes Tbx21
T7262 11119-11124 Protein denotes T-bet
T7263 11126-11135 Negative_regulation denotes deficient
T7264 11170-11175 Protein denotes Tbx21
T7265 11179-11182 Protein denotes CD8
T7266 11192-11200 Gene_expression denotes produced true
T7267 11201-11206 Protein denotes IFN-γ
T7268 11278-11283 Protein denotes T-bet
T7269 11284-11294 Negative_regulation denotes deficiency
T7270 11332-11335 Protein denotes CD8
T7271 11468-11473 Protein denotes Eomes
T7272 11494-11501 Positive_regulation denotes induced
T7273 11546-11551 Protein denotes T-bet
T7274 11552-11561 Negative_regulation denotes deficient
T7275 11597-11603 Negative_regulation denotes defect true
T7276 11607-11615 Protein denotes perforin
T7277 11616-11631 Transcription denotes mRNA expression
T7278 11701-11710 Negative_regulation denotes reduction
T7279 11714-11718 Protein denotes GzmB
T7280 11727-11732 Protein denotes T-bet
T7281 11733-11742 Negative_regulation denotes deficient
T7282 11817-11825 Negative_regulation denotes decrease
T7283 11829-11839 Gene_expression denotes expression
T7284 11843-11853 Protein denotes granzyme B
T7285 13153-13158 Protein denotes Eomes
T7286 13180-13183 Protein denotes CD8
T7287 13205-13210 Protein denotes Tbx21
T7288 13284-13287 Protein denotes GFP
T7289 13360-13365 Protein denotes Eomes
T7290 13367-13374 Protein denotes Eo-VP16
T7291 13437-13444 Protein denotes Eo-VP16
T7292 13464-13467 Protein denotes GFP
T7293 13480-13489 Positive_regulation denotes increased
T7294 13490-13498 Protein denotes perforin
T7295 13499-13509 Gene_expression denotes expression
T7296 13525-13530 Protein denotes T-bet
T7297 13531-13540 Negative_regulation denotes deficient
T7298 13541-13544 Protein denotes CD8
T7299 13601-13608 Protein denotes Eo-VP16
T7300 13614-13621 Negative_regulation denotes rescued
T7301 13632-13638 Negative_regulation denotes defect
T7302 13642-13647 Protein denotes IFN-γ
T7303 13648-13658 Gene_expression denotes production
T7304 13671-13676 Protein denotes T-bet
T7305 13677-13686 Negative_regulation denotes deficient
T7306 13687-13690 Protein denotes CD8
T7307 13721-13728 Protein denotes Eo-VP16
T7308 13737-13743 Positive_regulation denotes induce true
T7309 13744-13748 Protein denotes GzmB
T7310 13749-13764 Transcription denotes mRNA expression
T7311 13781-13786 Protein denotes T-bet
T7312 13787-13796 Negative_regulation denotes deficient
T7313 13822-13827 Protein denotes T-bet
T7314 13828-13838 Positive_regulation denotes dependence
T7315 13842-13846 Protein denotes GzmB
T7316 13847-13862 Transcription denotes mRNA expression
T7317 13892-13899 Protein denotes Eo-VP16
T11027 13902-13907 Protein denotes Runx3
T11028 13986-13995 Positive_regulation denotes induction
T11029 13999-14004 Protein denotes Eomes
T11030 14013-14018 Protein denotes Runx3
T11031 14029-14038 Gene_expression denotes expressed
T11032 14053-14056 Protein denotes CD8
T11033 14086-14091 Protein denotes T-bet
T11034 14092-14097 Protein denotes Runx3
T11035 14133-14136 Protein denotes CD4
T11036 14176-14181 Protein denotes Runx3
T11037 14227-14230 Protein denotes CD8
T11038 14245-14250 Protein denotes Runx3
T11039 14307-14312 Protein denotes Runx3
T11040 14360-14363 Protein denotes CD8
T11041 14477-14482 Protein denotes Runx3
T11042 14486-14489 Protein denotes CD8
T11043 14589-14599 Gene_expression denotes expression true
T11044 14589-14599 Gene_expression denotes expression true
T11045 14589-14599 Gene_expression denotes expression true
T11046 14603-14611 Protein denotes perforin
T11047 14613-14623 Protein denotes granzyme B
T11048 14629-14634 Protein denotes IFN-γ
T11049 14671-14679 Protein denotes perforin
T11050 14697-14707 Gene_expression denotes expression
T11051 14725-14737 Positive_regulation denotes undetectable true
T11052 14741-14746 Protein denotes Runx3
T11053 14797-14802 Protein denotes Runx3
T11054 14837-14842 Protein denotes Eomes
T11055 14843-14853 Gene_expression denotes expression true
T11056 14868-14873 Protein denotes T-bet
T11057 14874-14884 Gene_expression denotes expression
T11058 14889-14899 Negative_regulation denotes unimpaired true
T11059 14925-14930 Protein denotes Runx3
T11060 14935-14943 Positive_regulation denotes required
T11061 14935-14943 Positive_regulation denotes required
T11062 14935-14943 Positive_regulation denotes required
T11063 14956-14966 Gene_expression denotes production
T11064 14956-14966 Gene_expression denotes production
T11065 14956-14966 Gene_expression denotes production
T11066 14970-14975 Protein denotes IFN-γ
T11067 14985-14988 Protein denotes TNF
T11068 14992-14996 Protein denotes IL-2
T11069 15001-15004 Protein denotes CD8
T11070 16290-16295 Protein denotes T-bet
T11071 16299-16311 Positive_regulation denotes up-regulated
T11072 16322-16329 Positive_regulation denotes induces
T11073 16330-16335 Protein denotes Runx3
T11074 16349-16354 Protein denotes T-bet
T11075 16359-16364 Protein denotes Runx3
T11076 16378-16384 Positive_regulation denotes induce
T11077 16378-16384 Positive_regulation denotes induce
T11078 16385-16390 Protein denotes IFN-γ
T11079 16395-16402 Negative_regulation denotes silence
T11080 16395-16402 Negative_regulation denotes silence
T11081 16403-16407 Protein denotes IL-4
T11082 16492-16497 Protein denotes Runx3
T11083 16507-16516 Positive_regulation denotes necessary true
T11084 16521-16526 Protein denotes Eomes
T11085 16527-16536 Positive_regulation denotes induction
T11086 16569-16574 Protein denotes Eomes
T11087 16575-16585 Gene_expression denotes expression
T11088 16606-16614 Protein denotes perforin
T11089 16615-16625 Gene_expression denotes expression
T11090 16644-16658 Positive_regulation denotes overexpression
T11091 16662-16669 Protein denotes Eo-VP16
T11092 16686-16691 Protein denotes T-bet
T11093 16692-16701 Negative_regulation denotes deficient
T11094 16710-16713 Positive_regulation denotes led
T11095 16710-16713 Positive_regulation denotes led
T11096 16720-16728 Positive_regulation denotes increase
T11097 16720-16728 Positive_regulation denotes increase
T11098 16737-16745 Protein denotes perforin
T11099 16750-16755 Protein denotes IFN-γ
T11100 16756-16766 Gene_expression denotes expression
T11101 16756-16766 Gene_expression denotes expression
T11102 16982-16987 Protein denotes Runx3
T11103 16999-17008 Positive_regulation denotes necessary true
T11104 17013-17018 Protein denotes Eomes
T11105 17019-17028 Positive_regulation denotes induction
T11106 17051-17056 Protein denotes Eomes
T11107 17060-17068 Regulation denotes regulate
T11108 17060-17068 Regulation denotes regulate
T11109 17060-17068 Regulation denotes regulate
T11110 17060-17068 Regulation denotes regulate
T11111 17060-17068 Regulation denotes regulate
T11112 17060-17068 Regulation denotes regulate
T11113 17069-17082 Transcription denotes transcription
T11114 17069-17082 Transcription denotes transcription
T11115 17069-17082 Transcription denotes transcription
T11116 17111-17119 Protein denotes perforin
T11117 17121-17126 Protein denotes IFN-γ
T11118 17132-17142 Protein denotes granzyme B
T11119 17236-17241 Protein denotes Eomes
T11120 17246-17251 Protein denotes Runx3
T11121 17252-17257 Binding denotes bound true
T11122 17252-17257 Binding denotes bound true
T11123 17252-17257 Binding denotes bound true
T11124 17252-17257 Binding denotes bound true
T11125 17252-17257 Binding denotes bound true
T11126 17252-17257 Binding denotes bound true
T11127 17258-17276 Entity denotes regulatory regions
T11128 17284-17288 Protein denotes Prf1
T11129 17290-17294 Protein denotes Ifng
T11130 17300-17304 Protein denotes Gzmb
T11131 17337-17347 Binding denotes associated
T11132 17337-17347 Binding denotes associated
T11133 17401-17406 Protein denotes Runx3
T11134 17407-17412 Binding denotes bound
T11135 17407-17412 Binding denotes bound
T11136 17420-17424 Protein denotes Prf1
T11137 17429-17433 Protein denotes Gzmb
T11138 17434-17459 Entity denotes transcription start sites
T11139 17529-17533 Protein denotes Prf1
T11140 17609-17613 Protein denotes Prf1
T11141 17633-17637 Protein denotes Ifng
T11142 17755-17759 Protein denotes Ifng
T11143 17800-17805 Protein denotes Eomes
T11144 17806-17811 Binding denotes bound
T11145 17806-17811 Binding denotes bound
T11146 17829-17833 Protein denotes Prf1
T11147 17834-17837 Entity denotes TSS
T11148 17846-17860 Entity denotes −1 kb enhancer
T11149 17906-17910 Binding denotes that
T11150 17927-17935 Entity denotes promoter
T11151 17943-17948 Protein denotes Il2rb
T11152 17970-17976 Binding denotes target
T11153 17980-17985 Protein denotes Eomes
T11154 18009-18013 Binding denotes that
T11155 18030-18034 Protein denotes Ifng
T11156 18035-18038 Entity denotes TSS
T11157 18048-18054 Binding denotes target
T11158 18089-18092 Protein denotes CD8
T11159 18140-18145 Protein denotes Runx3
T11160 18206-18215 Positive_regulation denotes induction
T11161 18219-18224 Protein denotes Eomes
T11162 18242-18251 Gene_expression denotes expressed
T11163 18242-18251 Gene_expression denotes expressed
T11164 18252-18257 Protein denotes Runx3
T11165 18262-18269 Protein denotes Eo-VP16
T11166 18273-18276 Protein denotes CD8
T11167 18291-18296 Protein denotes Runx3
T11168 18339-18342 Protein denotes CD8
T11169 18408-18413 Protein denotes Runx3
T11170 18417-18420 Protein denotes CD8
T11171 18421-18424 Protein denotes CD4
T11172 18433-18436 Protein denotes CD8
T11173 18437-18440 Protein denotes CD4
T11174 18494-18499 Protein denotes Runx3
T11175 18503-18506 Protein denotes CD8
T11176 18607-18612 Protein denotes Runx3
T11177 18616-18619 Protein denotes CD8
T11178 18634-18639 Protein denotes Runx3
T11179 18640-18648 Positive_regulation denotes restored
T11180 18640-18648 Positive_regulation denotes restored
T11181 18640-18648 Positive_regulation denotes restored
T11182 18640-18648 Positive_regulation denotes restored
T11183 18649-18659 Gene_expression denotes expression
T11184 18649-18659 Gene_expression denotes expression
T11185 18649-18659 Gene_expression denotes expression
T11186 18649-18659 Gene_expression denotes expression
T11187 18663-18668 Protein denotes Eomes
T11188 18680-18688 Protein denotes perforin
T11189 18690-18700 Protein denotes granzyme B
T11190 18706-18711 Protein denotes IFN-γ
T11191 18744-18749 Protein denotes Runx3
T11192 18783-18796 Positive_regulation denotes up-regulation
T11193 18800-18805 Protein denotes Runx1
T11194 18817-18827 Negative_regulation denotes suppressed
T11195 18853-18858 Protein denotes Runx3
T11196 18876-18881 Protein denotes Runx1
T11197 18897-18907 Negative_regulation denotes repression
T11198 18911-18916 Protein denotes Runx3
T11199 18927-18934 Protein denotes Eo-VP16
T11200 18943-18954 Positive_regulation denotes up-regulate true
T11201 18955-18963 Protein denotes perforin
T11202 18964-18974 Gene_expression denotes expression
T11203 18980-18989 Gene_expression denotes expressed
T11204 18993-18998 Protein denotes Runx3
T11205 19024-19032 Positive_regulation denotes restored
T11206 19049-19055 Positive_regulation denotes induce
T11207 19056-19061 Protein denotes IFN-γ
T11208 19062-19072 Gene_expression denotes expression
T11209 19150-19158 Protein denotes perforin
T11210 19159-19169 Gene_expression denotes expression
T11211 19170-19178 Positive_regulation denotes requires true
T11212 19170-19178 Positive_regulation denotes requires true
T11213 19179-19184 Protein denotes Runx3
T11214 19189-19194 Protein denotes Eomes
T11215 20890-20896 Positive_regulation denotes defect true
T11216 20890-20896 Positive_regulation denotes defect true
T11217 20890-20896 Positive_regulation denotes defect true
T11218 20890-20896 Positive_regulation denotes defect true
T11219 20900-20908 Protein denotes perforin
T11220 20913-20923 Protein denotes granzyme B
T11221 20924-20934 Gene_expression denotes expression
T11222 20924-20934 Gene_expression denotes expression
T11223 20936-20941 Protein denotes Runx3
T11224 20945-20948 Protein denotes CD8
T11225 21055-21060 Protein denotes Runx3
T11226 21064-21067 Protein denotes CD8
T11227 21173-21176 Protein denotes CD8
T11228 21214-21219 Protein denotes Runx3
T11229 21322-21332 Positive_regulation denotes activation
T11230 21322-21332 Positive_regulation denotes activation
T11231 21340-21348 Protein denotes perforin
T11232 21349-21359 Protein denotes granzyme B
T11233 21373-21382 Negative_regulation denotes defective
T11234 21373-21382 Negative_regulation denotes defective
T11235 21386-21391 Protein denotes Runx3
T11236 21395-21398 Protein denotes CD8
T11237 21554-21557 Protein denotes Fas
T11238 21558-21568 Protein denotes Fas ligand
T13502 21579-21584 Protein denotes Runx3
T13503 21733-21738 Protein denotes Runx3
T13504 21865-21870 Protein denotes Runx3
T13505 21874-21881 Gene_expression denotes present
T13506 21891-21894 Protein denotes CD8
T13507 21931-21940 Negative_regulation denotes represses
T13508 21941-21946 Protein denotes Runx1
T13509 21951-21970 Positive_regulation denotes has a positive role
T13510 21951-21970 Positive_regulation denotes has a positive role
T13511 21951-21970 Positive_regulation denotes has a positive role
T13512 21951-21970 Positive_regulation denotes has a positive role
T13513 21978-21987 Positive_regulation denotes induction
T13514 21978-21987 Positive_regulation denotes induction
T13515 21978-21987 Positive_regulation denotes induction
T13516 21978-21987 Positive_regulation denotes induction
T13517 21991-21996 Protein denotes Eomes
T13518 21998-22008 Protein denotes granzyme B
T13519 22010-22018 Protein denotes perforin
T13520 22024-22029 Protein denotes IFN-γ
T13521 22031-22036 Protein denotes Runx3
T13522 22037-22042 Binding denotes binds
T13523 22037-22042 Binding denotes binds
T13524 22037-22042 Binding denotes binds
T13525 22037-22042 Binding denotes binds
T13526 22037-22042 Binding denotes binds
T13527 22037-22042 Binding denotes binds
T13528 22046-22055 Entity denotes promoters
T13529 22069-22087 Entity denotes regulatory regions
T13530 22135-22141 Regulation denotes effect true
T13531 22135-22141 Regulation denotes effect true
T13532 22135-22141 Regulation denotes effect true
T13533 22150-22160 Gene_expression denotes expression
T13534 22150-22160 Gene_expression denotes expression
T13535 22150-22160 Gene_expression denotes expression
T13536 22217-22222 Protein denotes Eomes
T13537 22227-22232 Protein denotes Runx1
T13538 22249-22255 Binding denotes target true
T13539 22249-22255 Binding denotes target true
T13540 22265-22270 Protein denotes Runx3
T13541 22286-22291 Protein denotes Runx3
T13542 22292-22303 Positive_regulation denotes contributed
T13543 22292-22303 Positive_regulation denotes contributed
T13544 22292-22303 Positive_regulation denotes contributed
T13545 22319-22329 Gene_expression denotes expression
T13546 22319-22329 Gene_expression denotes expression
T13547 22319-22329 Gene_expression denotes expression
T13548 22333-22336 Protein denotes TNF
T13549 22338-22342 Protein denotes IL-2
T13550 22348-22353 Protein denotes IFN-γ
T13551 22378-22381 Protein denotes TNF
T13552 22386-22390 Protein denotes IL-2
T13553 22396-22407 Positive_regulation denotes requirement
T13554 22396-22407 Positive_regulation denotes requirement
T13555 22412-22417 Protein denotes Runx3
T13556 22418-22426 Negative_regulation denotes subsides
T13557 22418-22426 Negative_regulation denotes subsides
T13558 22484-22489 Protein denotes Runx1
T13559 22500-22511 Negative_regulation denotes derepressed true
T13560 22515-22520 Protein denotes Runx3
T13561 22542-22547 Protein denotes Runx3
T13562 22564-22572 Positive_regulation denotes required
T13563 22577-22582 Protein denotes IFN-γ
T13564 22583-22593 Gene_expression denotes expression
T13565 22632-22636 Regulation denotes role true
T13566 22644-22653 Positive_regulation denotes induction
T13567 22657-22662 Protein denotes Eomes
T13568 22663-22673 Gene_expression denotes expression
T13569 22754-22759 Protein denotes T-bet
T13570 22764-22769 Protein denotes Eomes
T13571 22775-22787 Positive_regulation denotes up-regulated
T13572 22775-22787 Positive_regulation denotes up-regulated
T13573 22820-22823 Protein denotes CD8
T13574 22956-22961 Protein denotes T-bet
T13575 22965-22971 Positive_regulation denotes needed
T13576 23001-23004 Protein denotes CD8
T13577 23032-23039 Gene_expression denotes produce
T13578 23040-23045 Protein denotes IFN-γ
T13579 23117-23122 Protein denotes Eomes
T13580 23133-23140 Positive_regulation denotes induced
T13581 23150-23170 Regulation denotes functions downstream
T13582 23174-23179 Protein denotes Runx3
T13583 23200-23205 Protein denotes T-bet
T13584 23209-23218 Positive_regulation denotes promoting true
T13585 23209-23218 Positive_regulation denotes promoting true
T13586 23229-23239 Gene_expression denotes expression
T13587 23243-23248 Protein denotes IFN-γ
T13588 23283-23288 Protein denotes T-bet
T13589 23293-23298 Protein denotes Eomes
T13590 23304-23314 Positive_regulation denotes contribute
T13591 23304-23314 Positive_regulation denotes contribute
T13592 23318-23326 Protein denotes perforin
T13593 23327-23337 Gene_expression denotes expression
T13594 23363-23368 Protein denotes Eomes
T13595 23369-23376 Positive_regulation denotes induces
T13596 23369-23376 Positive_regulation denotes induces
T13597 23377-23387 Protein denotes granzyme B
T13598 23406-23411 Protein denotes T-bet
T13599 23555-23560 Protein denotes Eomes
T13600 23565-23570 Protein denotes T-bet
T13601 23613-23619 Positive_regulation denotes induce
T13602 23613-23619 Positive_regulation denotes induce
T13603 23613-23619 Positive_regulation denotes induce
T13604 23613-23619 Positive_regulation denotes induce
T13605 23655-23659 Protein denotes Prf1
T13606 23664-23668 Protein denotes Gzmb
T13607 23689-23694 Protein denotes T-bet
T13608 23699-23704 Protein denotes Eomes
T13609 23722-23732 Regulation denotes regulating
T13610 23722-23732 Regulation denotes regulating
T13611 23733-23743 Protein denotes granzyme B
T13612 23748-23756 Protein denotes perforin
T13613 23757-23767 Gene_expression denotes expression
T13614 23757-23767 Gene_expression denotes expression
T13615 23783-23796 Positive_regulation denotes up-regulation
T13616 23783-23796 Positive_regulation denotes up-regulation
T13617 23800-23805 Protein denotes T-bet
T13618 23810-23815 Protein denotes Eomes
T13619 23841-23849 Positive_regulation denotes preceded
T13620 23841-23849 Positive_regulation denotes preceded
T13621 23850-23863 Positive_regulation denotes up-regulation
T13622 23850-23863 Positive_regulation denotes up-regulation
T13623 23867-23871 Protein denotes Gzmb
T13624 23876-23880 Protein denotes Prf1
T13625 23913-23918 Protein denotes T-bet
T13626 23919-23930 Regulation denotes had no role
T13627 23934-23942 Protein denotes perforin
T13628 23943-23953 Gene_expression denotes expression
T13629 23988-23995 Protein denotes Eo-VP16
T13630 24004-24010 Regulation denotes affect true
T13631 24011-24021 Protein denotes granzyme B
T13632 24022-24032 Gene_expression denotes expression
T13633 24038-24047 Gene_expression denotes expressed
T13634 24051-24056 Protein denotes T-bet
T13635 24063-24068 Protein denotes Runx3
T13636 24100-24105 Protein denotes Eomes
T13637 24106-24115 Negative_regulation denotes deficient
T13638 24229-24238 Negative_regulation denotes deficient
T13639 24242-24247 Protein denotes Eomes
T13640 24315-24320 Protein denotes Runx3
T13641 24326-24331 Protein denotes Eomes
T13642 24332-24341 Negative_regulation denotes deficient
T13643 24342-24345 Protein denotes CD8
T13644 24380-24385 Protein denotes Eomes
T13645 24402-24407 Protein denotes Runx3
T13646 24411-24417 Positive_regulation denotes induce true
T13647 24411-24417 Positive_regulation denotes induce true
T13648 24418-24426 Protein denotes perforin
T13649 24427-24437 Gene_expression denotes expression
T13650 24529-24534 Protein denotes Runx3
T13651 24555-24562 Positive_regulation denotes induced
T13652 24555-24562 Positive_regulation denotes induced
T13653 24577-24582 Protein denotes T-bet
T13654 24587-24592 Protein denotes Eomes
T13655 24597-24603 Protein denotes IL-2Rβ
T13656 24713-24724 Regulation denotes interaction
T13657 24713-24724 Regulation denotes interaction
T13658 24713-24724 Regulation denotes interaction
T13659 24713-24724 Regulation denotes interaction
T13660 24733-24738 Protein denotes T-bet
T13661 24743-24748 Protein denotes Runx3
T13662 24781-24784 Protein denotes CD4
T13663 24869-24874 Protein denotes T-bet
T13664 24878-24885 Positive_regulation denotes induced
T13665 24905-24910 Protein denotes IFN-γ
T13666 24924-24931 Positive_regulation denotes induces
T13667 24932-24937 Protein denotes Runx3
T13668 24971-24974 Protein denotes CD8
T13669 24997-25002 Protein denotes Runx3
T13670 25006-25014 Positive_regulation denotes required
T13671 25018-25024 Positive_regulation denotes induce
T13672 25056-25061 Protein denotes Eomes
T14314 25428-25432 Protein denotes IL-2
T14315 25439-25444 Protein denotes IFN-γ
T14316 25451-25454 Protein denotes TNF
T14317 25461-25471 Protein denotes granzyme B
T14318 25478-25481 Protein denotes CD8
T14319 25488-25492 Protein denotes CD25
T14320 25503-25507 Protein denotes CD44
T14321 25514-25518 Protein denotes CD69
T14322 25573-25578 Protein denotes Eomes
T14323 25625-25630 Protein denotes Runx3
T14324 25738-25746 Protein denotes perforin
T14325 25761-25766 Protein denotes Eomes
T14326 25830-25835 Protein denotes T-bet
T14327 25999-26008 Protein denotes Annexin V
T14328 26044-26047 Protein denotes CD8
T14329 26085-26088 Protein denotes CD8
T14884 26357-26360 Protein denotes CD8
T14885 26386-26390 Protein denotes Tcra
T14886 26442-26447 Protein denotes Tbx21
T14887 26585-26588 Protein denotes CD8
T14888 26603-26608 Protein denotes Runx3
T14889 26926-26929 Protein denotes CD8
T14890 27024-27027 Protein denotes CD3
T16026 27733-27738 Protein denotes Runx3
T16027 27786-27789 Protein denotes CD8
T16028 27790-27793 Protein denotes CD4
T16029 27852-27855 Protein denotes CD8
T16030 27856-27859 Protein denotes CD4
T16031 27914-27917 Protein denotes CD3
T16032 27924-27928 Protein denotes CD28
T16033 28028-28032 Protein denotes IL-2
T16034 28087-28092 Protein denotes Runx3
T16035 28096-28099 Protein denotes CD8
T16036 28148-28151 Protein denotes CD4
T16037 28152-28162 Gene_expression denotes expression
T16038 28196-28201 Protein denotes Runx3
T16039 28292-28305 Positive_regulation denotes up-regulation
T16040 28292-28305 Positive_regulation denotes up-regulation
T16041 28309-28313 Protein denotes CD25
T16042 28318-28322 Protein denotes CD69
T16043 28368-28379 Positive_regulation denotes up-regulate
T16044 28380-28384 Protein denotes CD25
T16045 28386-28391 Protein denotes Runx3
T16046 28395-28398 Protein denotes CD8
T16047 28421-28425 Protein denotes IL-2
T16048 28610-28618 Negative_regulation denotes silenced
T16049 28619-28622 Protein denotes CD4
T16050 28623-28633 Gene_expression denotes expression
T16051 28932-28937 Protein denotes Runx3
T16052 28941-28944 Protein denotes CD8
T16053 28964-28973 Gene_expression denotes coexpress
T16054 28974-28977 Protein denotes CD4
T16055 29063-29068 Protein denotes Runx3
T16056 29134-29139 Protein denotes Runx3
T16057 29143-29146 Protein denotes CD8
T17549 31099-31102 Protein denotes CD8
T17550 32123-32128 Protein denotes Eomes
T17551 32150-32155 Protein denotes Runx3
T19018 6581-6584 Protein denotes CD8
T19019 6626-6630 Protein denotes Prf1
T19020 6632-6636 Protein denotes Gzmb
T19021 6638-6643 Protein denotes Tbx21
T19022 6645-6650 Protein denotes T-bet
T19023 6657-6662 Protein denotes Eomes
T19024 6663-6678 Transcription denotes mRNA expression true
T19025 6663-6678 Transcription denotes mRNA expression true
T19026 6663-6678 Transcription denotes mRNA expression true
T19027 6663-6678 Transcription denotes mRNA expression true
T19028 6702-6705 Protein denotes CD8
T19029 6965-6968 Protein denotes CD8
T19030 7126-7134 Gene_expression denotes staining true
T19031 7126-7134 Gene_expression denotes staining true
T19032 7126-7134 Gene_expression denotes staining true
T19033 7139-7149 Protein denotes granzyme B
T19034 7151-7156 Protein denotes IFN-γ
T19035 7162-7165 Protein denotes TNF
T19036 7167-7177 Protein denotes Granzyme B
T19037 7178-7186 Gene_expression denotes staining true
T19038 7361-7364 Protein denotes CD8
T19039 7485-7494 Protein denotes Annexin V
T19040 7528-7531 Protein denotes CD8
T19041 7730-7738 Protein denotes perforin
T19042 7739-7749 Protein denotes granzyme B
T19976 11884-11894 Regulation denotes Regulation
T19977 11884-11894 Regulation denotes Regulation
T19978 11884-11894 Regulation denotes Regulation
T19979 11884-11894 Regulation denotes Regulation
T19980 11884-11894 Regulation denotes Regulation
T19981 11884-11894 Regulation denotes Regulation
T19982 11898-11906 Protein denotes perforin
T19983 11908-11918 Protein denotes granzyme B
T19984 11924-11929 Protein denotes IFN-γ
T19985 11930-11940 Gene_expression denotes expression
T19986 11930-11940 Gene_expression denotes expression
T19987 11930-11940 Gene_expression denotes expression
T19988 11944-11949 Protein denotes T-bet
T19989 11954-11959 Protein denotes Eomes
T19990 11989-11994 Protein denotes IFN-γ
T19991 11995-12005 Gene_expression denotes expression true
T19992 12013-12018 Protein denotes Tbx21
T19993 12027-12032 Protein denotes T-bet
T19994 12033-12042 Negative_regulation denotes deficient
T19995 12044-12049 Protein denotes Tbx21
T19996 12069-12072 Protein denotes CD8
T19997 12183-12188 Protein denotes IFN-γ
T19998 12189-12199 Gene_expression denotes expression true
T19999 12271-12276 Protein denotes IFN-γ
T20000 12315-12319 Protein denotes Prf1
T20001 12324-12328 Protein denotes GzmB
T20002 12329-12344 Transcription denotes mRNA expression true
T20003 12329-12344 Transcription denotes mRNA expression true
T20004 12354-12359 Protein denotes T-bet
T20005 12360-12369 Negative_regulation denotes deficient
T20006 12370-12373 Protein denotes CD8
T20007 12460-12470 Gene_expression denotes expressing
T20008 12471-12481 Protein denotes Eomes-VP16
T20009 12483-12490 Protein denotes Eo-VP16
T20010 12509-12512 Protein denotes GFP
T20011 12707-12717 Protein denotes Granzyme B
T20012 12722-12727 Protein denotes IFN-γ
T20013 12728-12738 Gene_expression denotes expression true
T20014 12728-12738 Gene_expression denotes expression true
T20015 12742-12747 Protein denotes Tbx21
T20016 12755-12760 Protein denotes Tbx21
T20017 12839-12844 Protein denotes IFN-γ
T20018 12845-12855 Gene_expression denotes production true
T20019 12881-12888 Protein denotes Eo-VP16
T20020 12901-12904 Protein denotes GFP
T20021 13024-13027 Protein denotes GFP
T20022 13029-13034 Protein denotes IFN-γ
T20666 15071-15076 Protein denotes Runx3
T20667 15134-15139 Protein denotes Runx3
T20668 15141-15146 Protein denotes Eomes
T20669 15148-15153 Protein denotes T-bet
T20670 15159-15167 Protein denotes perforin
T20671 15168-15178 Gene_expression denotes expression true
T20672 15168-15178 Gene_expression denotes expression true
T20673 15168-15178 Gene_expression denotes expression true
T20674 15168-15178 Gene_expression denotes expression true
T20675 15182-15187 Protein denotes Runx3
T20676 15198-15203 Protein denotes Runx3
T20677 15207-15210 Protein denotes CD8
T20678 15247-15254 Protein denotes β-Actin
T20679 15316-15320 Protein denotes Prf1
T20680 15321-15336 Transcription denotes mRNA expression true
T20681 15340-15345 Protein denotes Runx3
T20682 15356-15361 Protein denotes Runx3
T20683 15365-15368 Protein denotes CD8
T20684 15402-15409 Protein denotes β–Actin
T20685 15445-15455 Gene_expression denotes Expression true
T20686 15445-15455 Gene_expression denotes Expression true
T20687 15445-15455 Gene_expression denotes Expression true
T20688 15445-15455 Gene_expression denotes Expression true
T20689 15459-15469 Protein denotes granzyme B
T20690 15471-15476 Protein denotes IFN-γ
T20691 15478-15481 Protein denotes TNF
T20692 15487-15491 Protein denotes IL-2
T20693 15525-15530 Protein denotes Runx3
T20694 15541-15546 Protein denotes Runx3
T20695 15550-15553 Protein denotes CD8
T20696 15627-15637 Protein denotes granzyme B
T20697 15649-15652 Protein denotes GFP
T20698 15748-15755 Binding denotes binding true
T20699 15748-15755 Binding denotes binding true
T20700 15770-15775 Protein denotes Runx3
T20701 15780-15785 Protein denotes Eomes
T20702 15793-15797 Protein denotes Prf1
T20703 16034-16037 Protein denotes CD8
T20704 16124-16128 Protein denotes Prf1
T20705 16147-16152 Protein denotes Runx3
T20706 16175-16180 Protein denotes Eomes
T21972 19206-19211 Protein denotes Runx3
T21973 19212-19220 Regulation denotes controls
T21974 19212-19220 Regulation denotes controls
T21975 19212-19220 Regulation denotes controls
T21976 19212-19220 Regulation denotes controls
T21977 19221-19226 Protein denotes Eomes
T21978 19228-19236 Protein denotes perforin
T21979 19238-19248 Protein denotes granzyme B
T21980 19254-19259 Protein denotes IFN-γ
T21981 19260-19270 Gene_expression denotes expression
T21982 19260-19270 Gene_expression denotes expression
T21983 19260-19270 Gene_expression denotes expression
T21984 19260-19270 Gene_expression denotes expression
T21985 19289-19294 Protein denotes Runx3
T21986 19301-19306 Protein denotes Runx3
T21987 19310-19313 Protein denotes CD8
T21988 19393-19396 Protein denotes GFP
T21989 19429-19439 Protein denotes Eomes-VP16
T21990 19441-19448 Protein denotes Eo-VP16
T21991 19453-19456 Protein denotes Myc
T21992 19457-19462 Protein denotes Runx3
T21993 19464-19469 Protein denotes Runx3
T21994 19678-19692 Positive_regulation denotes Overexpression
T21995 19696-19706 Protein denotes Eomes-VP16
T21996 19735-19740 Protein denotes Eomes
T21997 19830-19834 Protein denotes VP16
T21998 19863-19873 Gene_expression denotes Expression true
T21999 19863-19873 Gene_expression denotes Expression true
T22000 19877-19887 Protein denotes granzyme B
T22001 19892-19897 Protein denotes IFN-γ
T22002 20120-20130 Protein denotes granzyme B
T22003 20131-20139 Gene_expression denotes staining true
T22004 20238-20241 Protein denotes GFP
T22005 20381-20386 Protein denotes Runx3
T22006 20406-20411 Protein denotes T-bet
T22007 20415-20422 Positive_regulation denotes induced
T22008 20445-20454 Positive_regulation denotes essential
T22009 20465-20470 Protein denotes IFN-γ
T22010 20471-20481 Gene_expression denotes expression
T22011 20483-20488 Protein denotes Runx3
T22012 20492-20499 Gene_expression denotes present
T22013 20509-20512 Protein denotes CD8
T22014 20526-20535 Negative_regulation denotes represses
T22015 20536-20541 Protein denotes Runx1
T22016 20546-20553 Positive_regulation denotes induces
T22017 20546-20553 Positive_regulation denotes induces
T22018 20546-20553 Positive_regulation denotes induces
T22019 20546-20553 Positive_regulation denotes induces
T22020 20554-20559 Protein denotes Eomes
T22021 20561-20569 Protein denotes perforin
T22022 20571-20581 Protein denotes granzyme B
T22023 20587-20592 Protein denotes IFN-γ
T22024 20593-20603 Gene_expression denotes expression
T22025 20593-20603 Gene_expression denotes expression
T22026 20593-20603 Gene_expression denotes expression
T22027 20593-20603 Gene_expression denotes expression
T22028 20605-20610 Protein denotes Eomes
T22029 20615-20626 Regulation denotes participate true
T22030 20646-20651 Protein denotes IFN-γ
T22031 20652-20662 Gene_expression denotes expression
T22032 20672-20677 Protein denotes Runx3
T22033 20682-20687 Protein denotes Eomes
T22034 20697-20702 Protein denotes T-bet
T22035 20721-20729 Positive_regulation denotes activate true
T22036 20721-20729 Positive_regulation denotes activate true
T22037 20721-20729 Positive_regulation denotes activate true true
T22038 20730-20738 Protein denotes perforin
T22039 20739-20749 Gene_expression denotes expression
T22040 20789-20795 Regulation denotes effect
T22041 20789-20795 Regulation denotes effect
T22042 20799-20804 Protein denotes T-bet
T22043 20805-20815 Negative_regulation denotes deficiency
T22044 20819-20823 Protein denotes Gzmb
T22045 20837-20847 Protein denotes granzyme B
T22046 20856-20866 Gene_expression denotes expression
T22047 20856-20866 Gene_expression denotes expression true
T618 824-834 Gene_expression denotes expression
T619 824-834 Gene_expression denotes expression
T620 890-895 Protein denotes IFN-γ
T621 897-905 Protein denotes perforin
T622 911-921 Protein denotes granzyme B
T623 1004-1009 Protein denotes Runx3
T16013 27446-27451 Protein denotes Runx3
T16014 27461-27466 Protein denotes Runx3
T16015 27467-27476 Negative_regulation denotes deficient
T16016 27493-27500 Negative_regulation denotes silence true
T16017 27501-27504 Protein denotes CD4
T16018 27505-27515 Gene_expression denotes expression
T16019 27603-27606 Protein denotes CD8
T16020 27621-27626 Protein denotes Runx3
T16021 27640-27643 Protein denotes CD8
T16022 27644-27647 Protein denotes CD4
T16023 27655-27658 Protein denotes CD8
T16024 27659-27662 Protein denotes CD4
T16025 27698-27701 Protein denotes CD4
T597 0-5 Protein denotes Runx3
T598 124-127 Protein denotes CD8
T599 338-346 Protein denotes perforin
T600 498-507 Positive_regulation denotes induction
T601 511-516 Protein denotes T-bet
T602 526-535 Positive_regulation denotes induction
T603 539-551 Protein denotes Eomesodermin
T604 553-558 Protein denotes Eomes
T605 594-602 Regulation denotes regulate
T606 594-602 Regulation denotes regulate
T607 632-650 Protein denotes interferon (IFN) γ
T608 651-661 Gene_expression denotes expression
T609 750-755 Protein denotes Runx3
T610 780-785 Protein denotes Runx3
T611 786-795 Regulation denotes regulates
T612 786-795 Regulation denotes regulates
T613 786-795 Regulation denotes regulates
T614 786-795 Regulation denotes regulates
T615 796-801 Protein denotes Eomes
T616 802-812 Gene_expression denotes expression
T617 824-834 Gene_expression denotes expression
T16929 30931-30941 Protein denotes Eomes-VP16
T18109 34389-34392 Protein denotes CD8
T18110 34482-34486 Protein denotes IL-2
T14891 27050-27054 Protein denotes CD28
T14892 27406-27412 Protein denotes rhIL-2
T16362 29319-29322 Protein denotes CD8
T16363 29477-29486 Protein denotes Annexin V
T16364 29501-29504 Protein denotes CD8
T16365 29608-29611 Protein denotes CD8
T16366 29663-29672 Protein denotes Annexin V
T16928 30624-30627 Protein denotes CD8
T18271 35102-35105 Protein denotes CD8
T18272 35120-35125 Protein denotes Runx3
T18273 35180-35185 Protein denotes Runx3
R10638 T13541 T13543 causeOf Runx3,contributed
R10639 T13545 T13543 themeOf expression,contributed
R10644 T13550 T13545 themeOf IFN-γ,expression
R10645 T13551 T13554 themeOf TNF,requirement
R10646 T13552 T13553 themeOf IL-2,requirement
R10647 T13553 T13557 themeOf requirement,subsides
R10648 T13554 T13556 themeOf requirement,subsides
R10649 T13555 T13554 causeOf Runx3,requirement
R10650 T13555 T13553 causeOf Runx3,requirement
R10651 T13558 T13556 causeOf Runx1,subsides
R10652 T13558 T13557 causeOf Runx1,subsides
R10653 T13558 T13559 themeOf Runx1,derepressed
R10654 T13561 T13562 causeOf Runx3,required
R10655 T13561 T13565 causeOf Runx3,role
R10656 T13563 T13564 themeOf IFN-γ,expression
R10657 T13564 T13562 themeOf expression,required
R10658 T13566 T13565 themeOf induction,role
R508 T601 T600 themeOf T-bet,induction
R510 T603 T602 themeOf Eomesodermin,induction
R511 T604 T603 equivalentTo Eomes,Eomesodermin
R512 T607 T608 themeOf interferon (IFN) γ,expression
R513 T608 T606 themeOf expression,regulate
R514 T608 T605 themeOf expression,regulate
R515 T610 T611 causeOf Runx3,regulates
R516 T610 T614 causeOf Runx3,regulates
R517 T610 T612 causeOf Runx3,regulates
R518 T610 T613 causeOf Runx3,regulates
R519 T615 T616 themeOf Eomes,expression
R520 T616 T611 themeOf expression,regulates
R521 T617 T614 themeOf expression,regulates
R522 T618 T612 themeOf expression,regulates
R523 T619 T613 themeOf expression,regulates
R524 T620 T617 themeOf IFN-γ,expression
R525 T621 T618 themeOf perforin,expression
R526 T622 T619 themeOf granzyme B,expression
R1998 T2561 T2559 themeOf IFN-γ,produce
R2001 T2562 T2560 themeOf TNF,produce
R2003 T2565 T2564 equivalentTo Eomes,Eomesodermin
R2007 T2569 T2567 themeOf Tbx21,deletion
R2008 T2570 T2569 equivalentTo T-bet,Tbx21
R2009 T2571 T2568 themeOf eomesodermin,deletion
R2011 T2576 T2574 themeOf T-bet,deficient
R2012 T2577 T2575 themeOf Eomes,deficient
R2013 T2578 T2581 themeOf T-bet,targeting
R2014 T2579 T2582 themeOf Eomes,targeting
R2018 T2585 T2588 themeOf Runx3,expressed
R2019 T2586 T2587 themeOf perforin,expressed
R2021 T2591 T2592 themeOf Runx3,expressed
R2022 T2591 T2594 themeOf Runx3,expression
R2025 T2594 T2595 themeOf expression,up-regulated
R2026 T2596 T2597 causeOf Runx3,regulation
R2027 T2596 T2598 causeOf Runx3,regulation
R2028 T2614 T2615 causeOf Runx3,required
R2029 T2599 T2597 themeOf Il4,regulation
R2030 T2600 T2598 themeOf Ifng,regulation
R2031 T2602 T2603 themeOf Runx3,deficient
R2032 T2614 T2616 causeOf Runx3,required
R2033 T2617 T2619 themeOf Eomes,expression
R2034 T2607 T2608 themeOf T-bet,induced
R2035 T2618 T2620 themeOf perforin,expression
R2036 T2609 T2610 themeOf Eomes,induced
R2037 T2609 T2611 causeOf Eomes,sustains
R2038 T2619 T2615 themeOf expression,required
R2039 T2612 T2613 themeOf IFN-γ,expression
R2040 T2613 T2611 themeOf expression,sustains
R2041 T2620 T2616 themeOf expression,required
R2042 T2621 T2623 themeOf Eomes,bind
R2043 T2622 T2624 themeOf Runx3,bind
R2044 T2625 T2623 themeOf Prf1,bind
R2045 T2629 T2630 themeOf T-bet,deficient
R2046 T2630 T2628 causeOf deficient,unaffected
R2047 T2631 T2633 causeOf lacking,decreased
R2048 T2631 T2634 causeOf lacking,decreased
R2049 T2632 T2631 themeOf Runx3,lacking
R2050 T2625 T2624 themeOf Prf1,bind
R2051 T2626 T2627 themeOf perforin,expression
R2052 T2635 T2633 themeOf expression,decreased
R2053 T2636 T2634 themeOf expression,decreased
R2054 T2637 T2635 themeOf IFN-γ,expression
R2055 T2627 T2628 themeOf expression,unaffected
R2056 T2638 T2636 themeOf granzyme B,expression
R2057 T2639 T2640 themeOf Runx3,binds
R2058 T2639 T2641 themeOf Runx3,binds
R2059 T2642 T2640 themeOf promoter regions,binds
R2060 T2642 T2643 partOf promoter regions,Ifng
R2061 T2642 T2641 themeOf promoter regions,binds
R2062 T2642 T2644 partOf promoter regions,Gzmb
R2063 T2645 T2646 causeOf Runx3,regulator
R2064 T2647 T2648 themeOf Gzmb,expression
R2065 T2648 T2646 themeOf expression,regulator
R2066 T2649 T2651 causeOf T-bet,promote
R2067 T2650 T2652 causeOf Eomes,promote
R2068 T2653 T2651 themeOf transcription,promote
R2069 T2654 T2652 themeOf transcription,promote
R2070 T2655 T2653 themeOf Ifng,transcription
R2071 T2656 T2654 themeOf Prf1,transcription
R2489 T3210 T3209 equivalentTo rhIL-2,IL-2
R3556 T4493 T4492 themeOf perforin,expression
R3557 T4494 T4491 themeOf granzyme B,expression
R3558 T4495 T4498 themeOf perforin,expression
R3559 T4496 T4497 themeOf granzyme B,expression
R3560 T4502 T4501 themeOf perforin,expression
R3561 T4503 T4500 themeOf perforin,expression
R3562 T4505 T4504 themeOf perforin,expression
R3563 T4505 T4507 themeOf perforin,mRNA expression
R3564 T4506 T4505 equivalentTo Prf1,perforin
R3565 T4507 T4508 themeOf mRNA expression,increase
R3566 T4507 T4509 themeOf mRNA expression,decrease
R3567 T4507 T4510 themeOf mRNA expression,reexpression
R3568 T4511 T4513 themeOf granzyme B,low or undetectable
R3569 T4511 T4514 themeOf granzyme B,up-regulated
R3570 T4511 T4515 themeOf granzyme B,increased
R3571 T4512 T4511 equivalentTo Gzmb,granzyme B
R3572 T4516 T4517 themeOf granzyme B,remained high
R3573 T4518 T4522 themeOf expressed,increased
R3574 T4519 T4523 themeOf expressed,increased
R3575 T4520 T4518 themeOf IFN-γ,expressed
R3576 T4521 T4519 themeOf TNF,expressed
R3577 T4525 T4524 themeOf perforin,express
R3578 T4526 T4527 themeOf granzyme B,expression
R3579 T4528 T4529 themeOf perforin,expression
R3580 T4529 T4530 themeOf expression,induced
R4618 T5801 T5804 themeOf T-bet,expression
R4619 T5802 T5803 themeOf Eomes,expression
R4620 T5805 T5812 causeOf T-bet,linked
R4621 T5805 T5807 causeOf T-bet,linked
R4622 T5805 T5810 causeOf T-bet,linked
R4623 T5805 T5820 themeOf T-bet,expression
R4624 T5806 T5808 causeOf Eomes,linked
R4625 T5806 T5809 causeOf Eomes,linked
R4626 T5806 T5811 causeOf Eomes,linked
R4627 T5806 T5819 themeOf Eomes,expression
R4628 T5813 T5812 themeOf regulation,linked
R4629 T5813 T5808 themeOf regulation,linked
R4630 T5814 T5810 themeOf regulation,linked
R4631 T5814 T5811 themeOf regulation,linked
R4632 T5815 T5807 themeOf regulation,linked
R4633 T5815 T5809 themeOf regulation,linked
R4634 T5816 T5813 themeOf Ifng,regulation
R4635 T5817 T5815 themeOf Prf1,regulation
R4636 T5818 T5814 themeOf GzmB,regulation
R4637 T5821 T5822 themeOf T-bet,detectable
R4638 T5821 T5824 themeOf T-bet,induced
R4639 T5821 T5826 themeOf T-bet,expressed
R4640 T5826 T5825 themeOf expressed,remained
R4641 T5827 T5828 themeOf Eomes,expression
R4642 T5827 T5832 themeOf Eomes,expression
R4643 T5828 T5829 themeOf expression,low or undetectable
R4644 T5832 T5831 themeOf expression,effect
R4645 T5834 T5833 themeOf Eomes,induction
R4646 T5835 T5836 themeOf T-bet,mRNA expression
R4647 T5838 T5837 themeOf GzmB,expression
R4648 T5839 T5840 themeOf Eomes,expressed
R4649 T5842 T5841 themeOf perforin,reexpression
R4650 T5845 T5846 causeOf T-bet,required
R4651 T5847 T5848 themeOf IFN-γ,production
R4652 T5848 T5846 themeOf production,required
R4653 T5849 T5850 causeOf Eomes,contribute
R4654 T5851 T5850 themeOf control,contribute
R4655 T5852 T5853 themeOf perforin,expression
R4656 T5853 T5851 themeOf expression,control
R4657 T5855 T5854 themeOf T-bet,induce
R4658 T5855 T5857 causeOf T-bet,induces
R4659 T5855 T5856 causeOf T-bet,induces
R4660 T5858 T5857 themeOf IFN-γ,induces
R4661 T5859 T5856 themeOf granzyme B,induces
R4662 T5860 T5861 themeOf Eomes,induced
R4663 T5860 T5863 causeOf Eomes,activates
R4664 T5864 T5865 themeOf perforin,expression
R4665 T5865 T5863 themeOf expression,activates
R5715 T7247 T7249 themeOf Perforin,expression
R5716 T7248 T7250 themeOf granzyme B,expression
R5717 T7249 T7251 themeOf expression,regulated
R5718 T7250 T7252 themeOf expression,regulated
R5719 T7253 T7252 causeOf T-bet,regulated
R5720 T7253 T7251 causeOf T-bet,regulated
R5721 T7257 T7256 themeOf IFN-γ,expression
R5722 T7258 T7255 themeOf perforin,expression
R5723 T7259 T7254 themeOf granzyme B,expression
R5724 T7261 T7263 themeOf Tbx21,deficient
R5725 T7262 T7261 equivalentTo T-bet,Tbx21
R5726 T7267 T7266 themeOf IFN-γ,produced
R5727 T7268 T7269 themeOf T-bet,deficiency
R5728 T7271 T7272 themeOf Eomes,induced
R5729 T7273 T7274 themeOf T-bet,deficient
R5730 T7274 T7275 causeOf deficient,defect
R5731 T7276 T7277 themeOf perforin,mRNA expression
R5732 T7277 T7275 themeOf mRNA expression,defect
R5733 T7279 T7278 themeOf GzmB,reduction
R5734 T7280 T7281 themeOf T-bet,deficient
R5735 T7281 T7278 causeOf deficient,reduction
R5736 T7283 T7282 themeOf expression,decrease
R5737 T7284 T7283 themeOf granzyme B,expression
R5738 T7290 T7289 equivalentTo Eo-VP16,Eomes
R5739 T7291 T7293 causeOf Eo-VP16,increased
R5740 T7294 T7295 themeOf perforin,expression
R5741 T7295 T7293 themeOf expression,increased
R5742 T7296 T7297 themeOf T-bet,deficient
R5743 T7299 T7300 causeOf Eo-VP16,rescued
R5744 T7301 T7300 themeOf defect,rescued
R5745 T7302 T7303 themeOf IFN-γ,production
R5746 T7303 T7301 themeOf production,defect
R5747 T7304 T7305 themeOf T-bet,deficient
R5748 T7305 T7301 causeOf deficient,defect
R5749 T7307 T7308 causeOf Eo-VP16,induce
R5750 T7309 T7310 themeOf GzmB,mRNA expression
R5751 T7310 T7308 themeOf mRNA expression,induce
R5752 T7311 T7312 themeOf T-bet,deficient
R5753 T7313 T7314 causeOf T-bet,dependence
R5754 T7315 T7316 themeOf GzmB,mRNA expression
R5755 T7316 T7314 themeOf mRNA expression,dependence
R8676 T11027 T11028 causeOf Runx3,induction
R8679 T11029 T11028 themeOf Eomes,induction
R8680 T11030 T11031 themeOf Runx3,expressed
R8684 T11133 T11135 themeOf Runx3,bound
R8685 T11046 T11043 themeOf perforin,expression
R8686 T11133 T11134 themeOf Runx3,bound
R8687 T11138 T11135 themeOf transcription start sites,bound
R8688 T11047 T11045 themeOf granzyme B,expression
R8689 T11048 T11044 themeOf IFN-γ,expression
R8690 T11049 T11050 themeOf perforin,expression
R8691 T11050 T11051 themeOf expression,undetectable
R8692 T11138 T11136 partOf transcription start sites,Prf1
R8693 T11054 T11055 themeOf Eomes,expression
R8694 T11056 T11057 themeOf T-bet,expression
R8695 T11057 T11058 themeOf expression,unimpaired
R8696 T11138 T11134 themeOf transcription start sites,bound
R8697 T11138 T11137 partOf transcription start sites,Gzmb
R8698 T11059 T11061 causeOf Runx3,required
R8699 T11059 T11060 causeOf Runx3,required
R8700 T11059 T11062 causeOf Runx3,required
R8701 T11143 T11149 themeOf Eomes,that
R8702 T11063 T11062 themeOf production,required
R8703 T11064 T11060 themeOf production,required
R8704 T11065 T11061 themeOf production,required
R8705 T11066 T11065 themeOf IFN-γ,production
R8706 T11067 T11064 themeOf TNF,production
R8707 T11068 T11063 themeOf IL-2,production
R8708 T11143 T11154 themeOf Eomes,that
R8709 T11070 T11071 themeOf T-bet,up-regulated
R8710 T11143 T11145 themeOf Eomes,bound
R8711 T11143 T11144 themeOf Eomes,bound
R8712 T11073 T11072 themeOf Runx3,induces
R8713 T11147 T11145 themeOf TSS,bound
R8714 T11074 T11077 causeOf T-bet,induce
R8715 T11074 T11080 causeOf T-bet,silence
R8716 T11075 T11076 causeOf Runx3,induce
R8717 T11075 T11079 causeOf Runx3,silence
R8718 T11078 T11077 themeOf IFN-γ,induce
R8719 T11078 T11076 themeOf IFN-γ,induce
R8720 T11081 T11080 themeOf IL-4,silence
R8721 T11081 T11079 themeOf IL-4,silence
R8722 T11147 T11146 partOf TSS,Prf1
R8723 T11082 T11083 causeOf Runx3,necessary
R8724 T11148 T11144 themeOf −1 kb enhancer,bound
R8725 T11084 T11085 themeOf Eomes,induction
R8726 T11085 T11083 themeOf induction,necessary
R8727 T11148 T11146 partOf −1 kb enhancer,Prf1
R8728 T11086 T11087 themeOf Eomes,expression
R8729 T11150 T11149 themeOf promoter,that
R8730 T11088 T11089 themeOf perforin,expression
R8731 T11090 T11094 causeOf overexpression,led
R8732 T11150 T11151 partOf promoter,Il2rb
R8733 T11090 T11095 causeOf overexpression,led
R8734 T11150 T11152 themeOf promoter,target
R8735 T11091 T11090 themeOf Eo-VP16,overexpression
R8736 T11092 T11093 themeOf T-bet,deficient
R8737 T11153 T11152 themeOf Eomes,target
R8738 T11096 T11094 themeOf increase,led
R8739 T11156 T11154 themeOf TSS,that
R8740 T11097 T11095 themeOf increase,led
R8741 T11098 T11101 themeOf perforin,expression
R8742 T11099 T11100 themeOf IFN-γ,expression
R8743 T11100 T11097 themeOf expression,increase
R8744 T11101 T11096 themeOf expression,increase
R8745 T11156 T11155 partOf TSS,Ifng
R8746 T11102 T11103 causeOf Runx3,necessary
R8747 T11102 T11110 causeOf Runx3,regulate
R8748 T11102 T11108 causeOf Runx3,regulate
R8749 T11102 T11112 causeOf Runx3,regulate
R8750 T11104 T11105 themeOf Eomes,induction
R8751 T11105 T11103 themeOf induction,necessary
R8752 T11156 T11157 themeOf TSS,target
R8753 T11106 T11109 causeOf Eomes,regulate
R8754 T11106 T11111 causeOf Eomes,regulate
R8755 T11106 T11107 causeOf Eomes,regulate
R8756 T11159 T11160 causeOf Runx3,induction
R8757 T11161 T11160 themeOf Eomes,induction
R8758 T11113 T11112 themeOf transcription,regulate
R8759 T11113 T11107 themeOf transcription,regulate
R8760 T11114 T11108 themeOf transcription,regulate
R8761 T11114 T11111 themeOf transcription,regulate
R8762 T11115 T11110 themeOf transcription,regulate
R8763 T11115 T11109 themeOf transcription,regulate
R8764 T11116 T11115 themeOf perforin,transcription
R8765 T11164 T11163 themeOf Runx3,expressed
R8766 T11117 T11114 themeOf IFN-γ,transcription
R8767 T11118 T11113 themeOf granzyme B,transcription
R8768 T11119 T11123 themeOf Eomes,bound
R8769 T11119 T11126 themeOf Eomes,bound
R8770 T11119 T11124 themeOf Eomes,bound
R8771 T11119 T11131 themeOf Eomes,associated
R8772 T11120 T11122 themeOf Runx3,bound
R8773 T11120 T11125 themeOf Runx3,bound
R8774 T11120 T11121 themeOf Runx3,bound
R8775 T11120 T11132 themeOf Runx3,associated
R8776 T11165 T11162 themeOf Eo-VP16,expressed
R8777 T11178 T11182 causeOf Runx3,restored
R8778 T11127 T11123 themeOf regulatory regions,bound
R8779 T11127 T11128 partOf regulatory regions,Prf1
R8780 T11127 T11126 themeOf regulatory regions,bound
R8781 T11127 T11129 partOf regulatory regions,Ifng
R8782 T11127 T11124 themeOf regulatory regions,bound
R8783 T11127 T11130 partOf regulatory regions,Gzmb
R8784 T11127 T11122 themeOf regulatory regions,bound
R8785 T11127 T11125 themeOf regulatory regions,bound
R8786 T11127 T11121 themeOf regulatory regions,bound
R8787 T11178 T11181 causeOf Runx3,restored
R8788 T11178 T11180 causeOf Runx3,restored
R8789 T11178 T11179 causeOf Runx3,restored
R8790 T11183 T11181 themeOf expression,restored
R8791 T11184 T11182 themeOf expression,restored
R8792 T11185 T11180 themeOf expression,restored
R8793 T11186 T11179 themeOf expression,restored
R8794 T11187 T11184 themeOf Eomes,expression
R8795 T11188 T11183 themeOf perforin,expression
R8796 T11189 T11185 themeOf granzyme B,expression
R8798 T11190 T11186 themeOf IFN-γ,expression
R8799 T11193 T11192 themeOf Runx1,up-regulation
R8800 T11193 T11194 themeOf Runx1,suppressed
R8801 T11195 T11194 causeOf Runx3,suppressed
R8803 T11196 T11197 themeOf Runx1,repression
R8807 T11198 T11197 causeOf Runx3,repression
R8808 T11199 T11200 causeOf Eo-VP16,up-regulate
R8811 T11199 T11203 themeOf Eo-VP16,expressed
R8813 T11199 T11205 causeOf Eo-VP16,restored
R8814 T11201 T11202 themeOf perforin,expression
R8815 T11202 T11200 themeOf expression,up-regulate
R8816 T11206 T11205 themeOf induce,restored
R8825 T11207 T11208 themeOf IFN-γ,expression
R8827 T11208 T11206 themeOf expression,induce
R8829 T11209 T11210 themeOf perforin,expression
R8830 T11210 T11211 themeOf expression,requires
R8831 T11210 T11212 themeOf expression,requires
R8832 T11213 T11211 causeOf Runx3,requires
R8838 T11213 T11216 causeOf Runx3,defect
R8839 T11213 T11217 causeOf Runx3,defect
R8840 T11214 T11212 causeOf Eomes,requires
R8841 T11214 T11218 causeOf Eomes,defect
R8842 T11214 T11215 causeOf Eomes,defect
R8844 T11219 T11222 themeOf perforin,expression
R8853 T11220 T11221 themeOf granzyme B,expression
R8854 T11221 T11217 themeOf expression,defect
R8859 T11221 T11215 themeOf expression,defect
R8861 T11222 T11216 themeOf expression,defect
R8865 T11222 T11218 themeOf expression,defect
R8867 T11229 T11233 themeOf activation,defective
R8890 T11230 T11234 themeOf activation,defective
R8892 T11231 T11230 themeOf perforin,activation
R8893 T11232 T11229 themeOf granzyme B,activation
R10590 T13504 T13505 themeOf Runx3,present
R10591 T13504 T13507 causeOf Runx3,represses
R10592 T13504 T13511 causeOf Runx3,has a positive role
R10593 T13504 T13512 causeOf Runx3,has a positive role
R10594 T13504 T13509 causeOf Runx3,has a positive role
R10595 T13504 T13510 causeOf Runx3,has a positive role
R10596 T13508 T13507 themeOf Runx1,represses
R10597 T13513 T13512 themeOf induction,has a positive role
R10598 T13514 T13510 themeOf induction,has a positive role
R10599 T13515 T13509 themeOf induction,has a positive role
R10600 T13516 T13511 themeOf induction,has a positive role
R10601 T13517 T13516 themeOf Eomes,induction
R10602 T13518 T13513 themeOf granzyme B,induction
R10603 T13518 T13535 themeOf granzyme B,expression
R10604 T13519 T13515 themeOf perforin,induction
R10605 T13519 T13533 themeOf perforin,expression
R10606 T13520 T13514 themeOf IFN-γ,induction
R10607 T13520 T13534 themeOf IFN-γ,expression
R10608 T13521 T13522 themeOf Runx3,binds
R10609 T13521 T13524 themeOf Runx3,binds
R10610 T13521 T13525 themeOf Runx3,binds
R10611 T13521 T13527 themeOf Runx3,binds
R10612 T13521 T13526 themeOf Runx3,binds
R10613 T13521 T13523 themeOf Runx3,binds
R10614 T13521 T13531 causeOf Runx3,effect
R10615 T13521 T13530 causeOf Runx3,effect
R10616 T13521 T13532 causeOf Runx3,effect
R10617 T13528 T13522 themeOf promoters,binds
R10618 T13528 T13518 partOf promoters,granzyme B
R10619 T13528 T13524 themeOf promoters,binds
R10620 T13528 T13519 partOf promoters,perforin
R10621 T13528 T13525 themeOf promoters,binds
R10622 T13528 T13520 partOf promoters,IFN-γ
R10623 T13529 T13527 themeOf regulatory regions,binds
R10624 T13529 T13518 partOf regulatory regions,granzyme B
R10625 T13529 T13526 themeOf regulatory regions,binds
R10626 T13529 T13519 partOf regulatory regions,perforin
R10627 T13529 T13523 themeOf regulatory regions,binds
R10628 T13529 T13520 partOf regulatory regions,IFN-γ
R10629 T13533 T13530 themeOf expression,effect
R10630 T13534 T13532 themeOf expression,effect
R10631 T13535 T13531 themeOf expression,effect
R10632 T13536 T13539 themeOf Eomes,target
R10633 T13537 T13538 themeOf Runx1,target
R10634 T13540 T13539 themeOf Runx3,target
R10635 T13540 T13538 themeOf Runx3,target
R10636 T13541 T13542 causeOf Runx3,contributed
R10637 T13541 T13544 causeOf Runx3,contributed
R10640 T13546 T13544 themeOf expression,contributed
R10641 T13547 T13542 themeOf expression,contributed
R10642 T13548 T13547 themeOf TNF,expression
R10643 T13549 T13546 themeOf IL-2,expression
R10679 T13594 T13596 causeOf Eomes,induces
R10680 T13597 T13595 themeOf granzyme B,induces
R10682 T13598 T13595 causeOf T-bet,induces
R10683 T13599 T13604 causeOf Eomes,induce
R10684 T13599 T13601 causeOf Eomes,induce
R10685 T13600 T13602 causeOf T-bet,induce
R10686 T13600 T13603 causeOf T-bet,induce
R10687 T13605 T13604 themeOf Prf1,induce
R10688 T13605 T13602 themeOf Prf1,induce
R10689 T13606 T13601 themeOf Gzmb,induce
R10690 T13606 T13603 themeOf Gzmb,induce
R10691 T13607 T13610 causeOf T-bet,regulating
R10692 T13608 T13609 causeOf Eomes,regulating
R10693 T13611 T13614 themeOf granzyme B,expression
R10694 T13612 T13613 themeOf perforin,expression
R10695 T13613 T13609 themeOf expression,regulating
R10696 T13614 T13610 themeOf expression,regulating
R10697 T13615 T13620 causeOf up-regulation,preceded
R10698 T13616 T13619 causeOf up-regulation,preceded
R10699 T13617 T13615 themeOf T-bet,up-regulation
R10700 T13618 T13616 themeOf Eomes,up-regulation
R10701 T13621 T13620 themeOf up-regulation,preceded
R10702 T13622 T13619 themeOf up-regulation,preceded
R10703 T13623 T13621 themeOf Gzmb,up-regulation
R10704 T13624 T13622 themeOf Prf1,up-regulation
R10705 T13625 T13626 causeOf T-bet,had no role
R10706 T13627 T13628 themeOf perforin,expression
R10707 T13628 T13626 themeOf expression,had no role
R10708 T13629 T13630 causeOf Eo-VP16,affect
R10709 T13629 T13633 themeOf Eo-VP16,expressed
R10710 T13631 T13632 themeOf granzyme B,expression
R10711 T13632 T13630 themeOf expression,affect
R10712 T13636 T13637 themeOf Eomes,deficient
R10713 T13639 T13638 themeOf Eomes,deficient
R10714 T13641 T13642 themeOf Eomes,deficient
R10715 T13644 T13647 causeOf Eomes,induce
R10716 T13645 T13646 causeOf Runx3,induce
R10717 T13648 T13649 themeOf perforin,expression
R10718 T13649 T13647 themeOf expression,induce
R10719 T13649 T13646 themeOf expression,induce
R10720 T13653 T13651 themeOf T-bet,induced
R10721 T13654 T13652 themeOf Eomes,induced
R10722 T13660 T13659 themeOf T-bet,interaction
R10723 T13660 T13659 causeOf T-bet,interaction
R10724 T13660 T13657 themeOf T-bet,interaction
R10725 T13660 T13656 causeOf T-bet,interaction
R10726 T13661 T13657 causeOf Runx3,interaction
R10727 T13661 T13656 themeOf Runx3,interaction
R10728 T13661 T13658 themeOf Runx3,interaction
R10729 T13661 T13658 causeOf Runx3,interaction
R10730 T13663 T13664 themeOf T-bet,induced
R10731 T13663 T13666 causeOf T-bet,induces
R10732 T13665 T13664 causeOf IFN-γ,induced
R10733 T13667 T13666 themeOf Runx3,induces
R10734 T13669 T13670 causeOf Runx3,required
R10735 T13671 T13670 themeOf induce,required
R10736 T13672 T13671 themeOf Eomes,induce
R12328 T16014 T16015 themeOf Runx3,deficient
R12329 T16015 T16016 causeOf deficient,silence
R12330 T16017 T16018 themeOf CD4,expression
R12331 T16018 T16016 themeOf expression,silence
R12332 T16036 T16037 themeOf CD4,expression
R12333 T16041 T16039 themeOf CD25,up-regulation
R12334 T16042 T16040 themeOf CD69,up-regulation
R12335 T16044 T16043 themeOf CD25,up-regulate
R12336 T16049 T16050 themeOf CD4,expression
R12337 T16050 T16048 themeOf expression,silenced
R12338 T16054 T16053 themeOf CD4,coexpress
R14774 T19019 T19025 themeOf Prf1,mRNA expression
R14775 T19020 T19027 themeOf Gzmb,mRNA expression
R14776 T19021 T19026 themeOf Tbx21,mRNA expression
R14777 T19022 T19021 equivalentTo T-bet,Tbx21
R14778 T19023 T19024 themeOf Eomes,mRNA expression
R14779 T19033 T19031 themeOf granzyme B,staining
R14780 T19034 T19032 themeOf IFN-γ,staining
R14781 T19035 T19030 themeOf TNF,staining
R14782 T19036 T19037 themeOf Granzyme B,staining
R15474 T19982 T19987 themeOf perforin,expression
R15475 T19983 T19986 themeOf granzyme B,expression
R15476 T19984 T19985 themeOf IFN-γ,expression
R15477 T19985 T19977 themeOf expression,Regulation
R15478 T19985 T19976 themeOf expression,Regulation
R15479 T19986 T19981 themeOf expression,Regulation
R15480 T19986 T19980 themeOf expression,Regulation
R15481 T19987 T19978 themeOf expression,Regulation
R15482 T19987 T19979 themeOf expression,Regulation
R15483 T19988 T19977 causeOf T-bet,Regulation
R15484 T19988 T19978 causeOf T-bet,Regulation
R15485 T19988 T19980 causeOf T-bet,Regulation
R15486 T19989 T19981 causeOf Eomes,Regulation
R15487 T19989 T19976 causeOf Eomes,Regulation
R15488 T19989 T19979 causeOf Eomes,Regulation
R15489 T19990 T19991 themeOf IFN-γ,expression
R15490 T19993 T19994 themeOf T-bet,deficient
R15491 T19997 T19998 themeOf IFN-γ,expression
R15492 T20000 T20003 themeOf Prf1,mRNA expression
R15493 T20001 T20002 themeOf GzmB,mRNA expression
R15494 T20004 T20005 themeOf T-bet,deficient
R15495 T20008 T20007 themeOf Eomes-VP16,expressing
R15496 T20009 T20008 equivalentTo Eo-VP16,Eomes-VP16
R15497 T20011 T20013 themeOf Granzyme B,expression
R15498 T20012 T20014 themeOf IFN-γ,expression
R15499 T20017 T20018 themeOf IFN-γ,production
R15854 T20667 T20673 themeOf Runx3,expression
R15855 T20668 T20674 themeOf Eomes,expression
R15856 T20669 T20671 themeOf T-bet,expression
R15857 T20670 T20672 themeOf perforin,expression
R15858 T20679 T20680 themeOf Prf1,mRNA expression
R15859 T20689 T20685 themeOf granzyme B,Expression
R15860 T20690 T20686 themeOf IFN-γ,Expression
R15861 T20691 T20688 themeOf TNF,Expression
R15862 T20692 T20687 themeOf IL-2,Expression
R15863 T20700 T20699 themeOf Runx3,binding
R15864 T20701 T20698 themeOf Eomes,binding
R15865 T20702 T20699 themeOf Prf1,binding
R15866 T20702 T20698 themeOf Prf1,binding
R16849 T21972 T21976 causeOf Runx3,controls
R16850 T21972 T21975 causeOf Runx3,controls
R16851 T21972 T21974 causeOf Runx3,controls
R16852 T21972 T21973 causeOf Runx3,controls
R16853 T21977 T21982 themeOf Eomes,expression
R16854 T21978 T21983 themeOf perforin,expression
R16855 T21979 T21981 themeOf granzyme B,expression
R16856 T21980 T21984 themeOf IFN-γ,expression
R16857 T21981 T21973 themeOf expression,controls
R16858 T21982 T21975 themeOf expression,controls
R16859 T21983 T21974 themeOf expression,controls
R16860 T21984 T21976 themeOf expression,controls
R16861 T21990 T21989 equivalentTo Eo-VP16,Eomes-VP16
R16862 T21995 T21994 themeOf Eomes-VP16,Overexpression
R16863 T22000 T21999 themeOf granzyme B,Expression
R16864 T22001 T21998 themeOf IFN-γ,Expression
R16865 T22002 T22003 themeOf granzyme B,staining
R16866 T22006 T22007 themeOf T-bet,induced
R16867 T22006 T22008 causeOf T-bet,essential
R16868 T22009 T22010 themeOf IFN-γ,expression
R16869 T22010 T22008 themeOf expression,essential
R16870 T22011 T22012 themeOf Runx3,present
R16871 T22011 T22014 causeOf Runx3,represses
R16872 T22011 T22019 causeOf Runx3,induces
R16873 T22011 T22017 causeOf Runx3,induces
R16874 T22011 T22018 causeOf Runx3,induces
R16875 T22011 T22016 causeOf Runx3,induces
R16876 T22015 T22014 themeOf Runx1,represses
R16877 T22020 T22026 themeOf Eomes,expression
R16878 T22021 T22027 themeOf perforin,expression
R16879 T22022 T22024 themeOf granzyme B,expression
R16880 T22023 T22025 themeOf IFN-γ,expression
R16881 T22024 T22018 themeOf expression,induces
R16882 T22025 T22016 themeOf expression,induces
R16883 T22026 T22019 themeOf expression,induces
R16884 T22027 T22017 themeOf expression,induces
R16885 T22028 T22029 causeOf Eomes,participate
R16886 T22030 T22031 themeOf IFN-γ,expression
R16887 T22031 T22029 themeOf expression,participate
R16888 T22032 T22036 causeOf Runx3,activate
R16889 T22033 T22035 causeOf Eomes,activate
R16890 T22034 T22037 causeOf T-bet,activate
R16891 T22038 T22039 themeOf perforin,expression
R16892 T22039 T22036 themeOf expression,activate
R16893 T22039 T22035 themeOf expression,activate
R16894 T22039 T22037 themeOf expression,activate
R16895 T22042 T22043 themeOf T-bet,deficiency
R16896 T22043 T22040 causeOf deficiency,effect
R16897 T22043 T22041 causeOf deficiency,effect
R16898 T22044 T22046 themeOf Gzmb,expression
R16899 T22045 T22047 themeOf granzyme B,expression
R16900 T22046 T22040 themeOf expression,effect
R16901 T22047 T22041 themeOf expression,effect
R507 T601 T606 causeOf T-bet,regulate
R509 T603 T605 causeOf Eomesodermin,regulate
R10659 T13567 T13568 themeOf Eomes,expression
R10660 T13568 T13566 themeOf expression,induction
R10661 T13569 T13572 themeOf T-bet,up-regulated
R10662 T13570 T13571 themeOf Eomes,up-regulated
R10663 T13574 T13575 causeOf T-bet,needed
R10664 T13577 T13575 themeOf produce,needed
R10665 T13578 T13577 themeOf IFN-γ,produce
R10666 T13579 T13580 themeOf Eomes,induced
R10667 T13579 T13581 themeOf Eomes,functions downstream
R10668 T13579 T13584 causeOf Eomes,promoting
R10669 T13582 T13581 causeOf Runx3,functions downstream
R10670 T13583 T13585 causeOf T-bet,promoting
R10671 T13586 T13584 themeOf expression,promoting
R10672 T13586 T13585 themeOf expression,promoting
R10673 T13587 T13586 themeOf IFN-γ,expression
R10674 T13588 T13591 causeOf T-bet,contribute
R10675 T13589 T13590 causeOf Eomes,contribute
R10676 T13592 T13593 themeOf perforin,expression
R10677 T13593 T13591 themeOf expression,contribute
R10678 T13593 T13590 themeOf expression,contribute
R10681 T13597 T13596 themeOf granzyme B,induces

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T624 0-24 Protein denotes Runx3 and T-box proteins
T625 124-128 Protein denotes CD8+
T626 338-346 Protein denotes perforin
T627 561-588 Protein denotes T-box transcription factors
T628 632-642 Protein denotes interferon
T629 644-647 Protein denotes IFN
T630 729-755 Protein denotes transcription factor Runx3
T631 780-785 Protein denotes Runx3
T632 890-895 Protein denotes IFN-γ
T3216 4449-4453 Protein denotes CD8+
T3217 4484-4488 Protein denotes CD8+
T3218 4502-4505 Protein denotes P14
T3219 4554-4562 Protein denotes anti-CD3
T3220 4567-4576 Protein denotes anti-CD28
T3221 4617-4629 Protein denotes Gp33 peptide
T3222 4681-4703 Protein denotes recombinant human IL-2
T3223 4705-4711 Protein denotes rhIL-2
T3224 4806-4810 Protein denotes CD8+
T3225 5106-5109 Protein denotes CD3
T3226 5115-5119 Protein denotes CD28
T3227 5257-5261 Protein denotes CD8+
T3228 5429-5433 Protein denotes CD8+
T4532 5501-5509 Protein denotes perforin
T4533 5514-5524 Protein denotes granzyme B
T4534 5478-5488 Gene_expression denotes expression
T4535 5478-5488 Gene_expression denotes expression
T4536 5674-5678 Protein denotes CD8+
T4537 5753-5766 Protein denotes perforin mRNA
T4538 5778-5786 Protein denotes perforin
T4539 5739-5749 Gene_expression denotes expression
T4540 5739-5749 Gene_expression denotes expression
T4541 5855-5863 Protein denotes perforin
T4542 5865-5869 Protein denotes Prf1
T4543 5876-5886 Transcription denotes expression
T4544 5876-5886 Transcription denotes expression
T4545 5895-5903 Positive_regulation denotes increase
T4546 5895-5903 Positive_regulation denotes increase
T4547 5951-5959 Negative_regulation denotes decrease
T4548 5951-5959 Negative_regulation denotes decrease
T4549 5989-6001 Positive_regulation denotes reexpression
T4550 5989-6001 Positive_regulation denotes reexpression
T4551 6051-6061 Protein denotes granzyme B
T4552 6063-6067 Protein denotes Gzmb
T4553 6242-6260 Protein denotes granzyme B protein
T4554 6085-6097 Transcription denotes undetectable
T4555 6085-6097 Transcription denotes undetectable
T4556 6132-6144 Positive_regulation denotes up-regulated
T4557 6132-6144 Positive_regulation denotes up-regulated
T4558 6176-6185 Positive_regulation denotes increased
T4559 6176-6185 Positive_regulation denotes increased
T4560 6265-6274 Gene_expression denotes expressed
T4561 6297-6301 Positive_regulation denotes high
T4562 6397-6402 Protein denotes IFN-γ
T4563 6407-6410 Protein denotes TNF
T4564 6525-6533 Protein denotes Fig. 2 A
T4565 6373-6382 Gene_expression denotes expressed
T4566 6373-6382 Gene_expression denotes expressed
T4567 6516-6519 Gene_expression denotes see
T4568 6457-6466 Positive_regulation denotes increased
T4569 8007-8010 Protein denotes TCR
T4570 8294-8302 Protein denotes perforin
T4571 8254-8261 Gene_expression denotes express
T4572 8744-8748 Protein denotes TCRs
T4573 8788-8798 Protein denotes granzyme B
T4574 8886-8894 Protein denotes perforin
T4575 8988-8992 Protein denotes IL-2
T4576 8799-8809 Gene_expression denotes expression
T4577 8895-8905 Gene_expression denotes expression
T4578 8933-8940 Positive_regulation denotes induced
T5866 9384-9388 Protein denotes Prf1
T5867 9393-9397 Protein denotes GzmB
T5868 9273-9283 Regulation denotes regulation
T5869 9273-9283 Regulation denotes regulation
T5870 9571-9575 Protein denotes CD8+
T5871 9616-9619 Protein denotes TCR
T5872 9841-9845 Protein denotes CD8+
T5873 9859-9862 Protein denotes TCR
T5874 9914-9924 Gene_expression denotes expression
T5875 9900-9906 Regulation denotes effect
T5876 9973-9983 Protein denotes Eomes mRNA
T5877 9960-9969 Positive_regulation denotes induction
T5878 10110-10119 Protein denotes GzmB mRNA
T5879 10132-10142 Protein denotes Eomes mRNA
T5880 10204-10217 Protein denotes perforin mRNA
T5881 10096-10106 Gene_expression denotes expression
T5882 10160-10169 Gene_expression denotes expressed
T5883 10188-10200 Gene_expression denotes reexpression
T5884 10083-10091 Positive_regulation denotes preceded
T5885 10465-10470 Protein denotes IFN-γ
T5886 10613-10621 Protein denotes perforin
T5887 10471-10481 Gene_expression denotes production
T5888 10622-10632 Gene_expression denotes expression
T5889 10446-10454 Positive_regulation denotes required
T5890 10602-10609 Regulation denotes control
T5891 10578-10588 Positive_regulation denotes contribute
T5892 10688-10691 Protein denotes TCR
T5893 10736-10741 Protein denotes IFN-γ
T5894 10844-10848 Protein denotes IL-2
T5895 10863-10871 Protein denotes perforin
T5896 10700-10706 Positive_regulation denotes induce
T5897 10728-10735 Positive_regulation denotes induces
T5898 10872-10882 Gene_expression denotes expression
T5899 10853-10862 Positive_regulation denotes activates
T7318 10885-10893 Protein denotes Perforin
T7319 10898-10908 Protein denotes granzyme B
T7320 10909-10919 Gene_expression denotes expression
T7321 10909-10919 Gene_expression denotes expression
T7322 10940-10949 Regulation denotes regulated
T7323 10940-10949 Regulation denotes regulated
T7324 10940-10949 Regulation denotes regulated
T7325 11052-11057 Protein denotes IFN-γ
T7326 11059-11067 Protein denotes perforin
T7327 11073-11083 Protein denotes granzyme B
T7328 11087-11091 Protein denotes CD8+
T7329 11105-11107 Protein denotes WT
T7330 11112-11117 Protein denotes Tbx21
T7331 11038-11048 Gene_expression denotes expression
T7332 11038-11048 Gene_expression denotes expression
T7333 11038-11048 Gene_expression denotes expression
T7334 11125-11135 Negative_regulation denotes -deficient
T7335 11125-11135 Negative_regulation denotes -deficient
T7336 11179-11182 Protein denotes CD8
T7337 11201-11206 Protein denotes IFN-γ
T7338 11192-11200 Gene_expression denotes produced
T7339 11332-11336 Protein denotes CD8+
T7340 11607-11620 Protein denotes perforin mRNA
T7341 11621-11631 Gene_expression denotes expression
T7342 11597-11603 Negative_regulation denotes defect
T7343 11714-11723 Protein denotes GzmB mRNA
T7344 11843-11861 Protein denotes granzyme B protein
T7345 11863-11866 Protein denotes Fig
T7346 11701-11710 Negative_regulation denotes reduction
T7347 11829-11839 Gene_expression denotes expression
T7348 11829-11839 Gene_expression denotes expression
T7349 11817-11825 Negative_regulation denotes decrease
T7350 11817-11825 Negative_regulation denotes decrease
T7351 13153-13158 Protein denotes Eomes
T7352 13180-13184 Protein denotes CD8+
T7353 13198-13200 Protein denotes WT
T7354 13205-13210 Protein denotes Tbx21
T7355 13440-13444 Protein denotes VP16
T7356 13490-13498 Protein denotes perforin
T7357 13541-13544 Protein denotes CD8
T7358 13499-13509 Gene_expression denotes expression
T7359 13480-13489 Positive_regulation denotes increased
T7360 13601-13608 Protein denotes Eo-VP16
T7361 13642-13647 Protein denotes IFN-γ
T7362 13687-13690 Protein denotes CD8
T7363 13648-13658 Gene_expression denotes production
T7364 13632-13638 Negative_regulation denotes defect
T7365 13721-13728 Protein denotes Eo-VP16
T7366 13744-13753 Protein denotes GzmB mRNA
T7367 13842-13851 Protein denotes GzmB mRNA
T7368 13892-13899 Protein denotes Eo-VP16
T7369 13754-13764 Gene_expression denotes expression
T7370 13852-13862 Gene_expression denotes expression
T7371 13737-13743 Positive_regulation denotes induce
T11239 13902-13907 Protein denotes Runx3
T11240 14013-14018 Protein denotes Runx3
T11241 14053-14057 Protein denotes CD8+
T11242 14086-14097 Protein denotes T-bet-Runx3
T11243 14133-14137 Protein denotes CD4+
T11244 14176-14181 Protein denotes Runx3
T11245 14029-14038 Gene_expression denotes expressed
T11246 14227-14230 Protein denotes CD8
T11247 14245-14250 Protein denotes Runx3
T11248 14304-14306 Protein denotes WT
T11249 14307-14312 Protein denotes Runx3
T11250 14425-14428 Protein denotes org
T11251 14429-14432 Protein denotes cgi
T11252 14477-14482 Protein denotes Runx3
T11253 14486-14489 Protein denotes CD8
T11254 14603-14611 Protein denotes perforin
T11255 14613-14623 Protein denotes granzyme B
T11256 14629-14634 Protein denotes IFN-γ
T11257 14636-14639 Protein denotes Fig
T11258 14589-14599 Gene_expression denotes expression
T11259 14589-14599 Gene_expression denotes expression
T11260 14589-14599 Gene_expression denotes expression
T11261 14589-14599 Gene_expression denotes expression
T11262 14671-14684 Protein denotes perforin mRNA
T11263 14741-14746 Protein denotes Runx3
T11264 14697-14707 Gene_expression denotes expression
T11265 14725-14737 Positive_regulation denotes undetectable
T11266 14797-14802 Protein denotes Runx3
T11267 14925-14930 Protein denotes Runx3
T11268 14970-14975 Protein denotes IFN-γ
T11269 14985-14988 Protein denotes TNF
T11270 14992-14996 Protein denotes IL-2
T11271 15001-15005 Protein denotes CD8+
T11272 14956-14966 Gene_expression denotes production
T11273 14956-14966 Gene_expression denotes production
T11274 14956-14966 Gene_expression denotes production
T11275 14935-14943 Positive_regulation denotes required
T11276 14935-14943 Positive_regulation denotes required
T11277 14935-14943 Positive_regulation denotes required
T11278 16330-16335 Protein denotes Runx3
T11279 16359-16364 Protein denotes Runx3
T11280 16385-16407 Protein denotes IFN-γ and silence IL-4
T11281 16322-16329 Positive_regulation denotes induces
T11282 16492-16497 Protein denotes Runx3
T11283 16606-16614 Protein denotes perforin
T11284 16662-16664 Protein denotes Eo
T11285 16665-16669 Protein denotes VP16
T11286 16737-16745 Protein denotes perforin
T11287 16750-16755 Protein denotes IFN-γ
T11288 16913-16917 Protein denotes Runx
T11289 16922-16949 Protein denotes T-box transcription factors
T11290 16615-16625 Gene_expression denotes expression
T11291 16644-16658 Positive_regulation denotes overexpression
T11292 16644-16658 Positive_regulation denotes overexpression
T11293 16720-16728 Positive_regulation denotes increase
T11294 16756-16766 Gene_expression denotes expression
T11295 16756-16766 Gene_expression denotes expression
T11296 16877-16886 Regulation denotes involving
T11297 16877-16886 Regulation denotes involving
T11298 16710-16713 Positive_regulation denotes led
T11299 16710-16713 Positive_regulation denotes led
T11300 16720-16728 Positive_regulation denotes increase
T11301 16720-16728 Positive_regulation denotes increase
T11302 16982-16987 Protein denotes Runx3
T11303 17111-17119 Protein denotes perforin
T11304 17121-17126 Protein denotes IFN-γ
T11305 17132-17142 Protein denotes granzyme B
T11306 17069-17082 Transcription denotes transcription
T11307 17069-17082 Transcription denotes transcription
T11308 17069-17082 Transcription denotes transcription
T11309 17060-17068 Regulation denotes regulate
T11310 17060-17068 Regulation denotes regulate
T11311 17060-17068 Regulation denotes regulate
T11312 17236-17241 Protein denotes Eomes
T11313 17246-17251 Protein denotes Runx3
T11314 17284-17288 Protein denotes Prf1
T11315 17290-17294 Protein denotes Ifng
T11316 17300-17310 Protein denotes Gzmb genes
T11317 17312-17315 Protein denotes Fig
T11318 17269-17276 Entity denotes regions
T11319 17252-17257 Binding denotes bound
T11320 17252-17257 Binding denotes bound
T11321 17252-17257 Binding denotes bound
T11322 17252-17257 Binding denotes bound
T11323 17252-17257 Binding denotes bound
T11324 17252-17257 Binding denotes bound
T11325 17401-17406 Protein denotes Runx3
T11326 17420-17424 Protein denotes Prf1
T11327 17429-17433 Protein denotes Gzmb
T11328 17529-17533 Protein denotes Prf1
T11329 17572-17579 Protein denotes DNase I
T11330 17609-17613 Protein denotes Prf1
T11331 17633-17646 Protein denotes Ifng promoter
T11332 17719-17726 Protein denotes DNase I
T11333 17407-17412 Binding denotes bound
T11334 17829-17837 Protein denotes Prf1 TSS
T11335 17943-17953 Protein denotes Il2rb gene
T11336 18030-18038 Protein denotes Ifng TSS
T11337 18058-18072 Protein denotes T-box proteins
T11338 18081-18084 Protein denotes Th1
T11339 18089-18093 Protein denotes CD8+
T11340 17852-17860 Entity denotes enhancer
T11341 17927-17935 Entity denotes promoter
T11342 17970-17976 Binding denotes target
T11343 18048-18054 Binding denotes target
T11344 17806-17811 Binding denotes bound
T11345 18140-18145 Protein denotes Runx3
T11346 18175-18193 Protein denotes CTL effector genes
T11347 18252-18257 Protein denotes Runx3
T11348 18262-18269 Protein denotes Eo-VP16
T11349 18273-18277 Protein denotes CD8+
T11350 18291-18296 Protein denotes Runx3
T11351 18161-18171 Gene_expression denotes expression
T11352 18242-18251 Gene_expression denotes expressed
T11353 18242-18251 Gene_expression denotes expressed
T11354 18146-18156 Regulation denotes controlled
T11355 18339-18343 Protein denotes CD8+
T11356 18408-18413 Protein denotes Runx3
T11357 18417-18420 Protein denotes CD8
T11358 18421-18424 Protein denotes CD4
T11359 18426-18428 Protein denotes SP
T11360 18433-18436 Protein denotes CD8
T11361 18437-18440 Protein denotes CD4
T11362 18494-18499 Protein denotes Runx3
T11363 18503-18506 Protein denotes CD8
T11364 18607-18612 Protein denotes Runx3
T11365 18616-18619 Protein denotes CD8
T11366 18634-18639 Protein denotes Runx3
T11367 18680-18688 Protein denotes perforin
T11368 18690-18700 Protein denotes granzyme B
T11369 18706-18711 Protein denotes IFN-γ
T11370 18713-18716 Protein denotes Fig
T11371 18640-18648 Positive_regulation denotes restored
T11372 18649-18659 Gene_expression denotes expression
T11373 18649-18659 Gene_expression denotes expression
T11374 18649-18659 Gene_expression denotes expression
T11375 18649-18659 Gene_expression denotes expression
T11376 18640-18648 Positive_regulation denotes restored
T11377 18640-18648 Positive_regulation denotes restored
T11378 18640-18648 Positive_regulation denotes restored
T11379 18640-18648 Positive_regulation denotes restored
T11380 18744-18749 Protein denotes Runx3
T11381 18800-18805 Protein denotes Runx1
T11382 18853-18858 Protein denotes Runx3
T11383 18876-18881 Protein denotes Runx1
T11384 18911-18916 Protein denotes Runx3
T11385 18783-18796 Positive_regulation denotes up-regulation
T11386 18817-18827 Negative_regulation denotes suppressed
T11387 18897-18907 Negative_regulation denotes repression
T11388 18927-18934 Protein denotes Eo-VP16
T11389 18955-18963 Protein denotes perforin
T11390 18993-18998 Protein denotes Runx3
T11391 19056-19061 Protein denotes IFN-γ
T11392 19078-19081 Protein denotes TCR
T11393 18964-18974 Gene_expression denotes expression
T11394 19062-19072 Gene_expression denotes expression
T11395 19082-19095 Positive_regulation denotes restimulation
T11396 18943-18954 Positive_regulation denotes up-regulate
T11397 19049-19055 Positive_regulation denotes induce
T11398 19024-19032 Positive_regulation denotes restored
T11399 19150-19158 Protein denotes perforin
T11400 19179-19184 Protein denotes Runx3
T11401 19189-19194 Protein denotes Eomes
T11402 19159-19169 Gene_expression denotes expression
T11403 20900-20908 Protein denotes perforin
T11404 20913-20923 Protein denotes granzyme B
T11405 20936-20941 Protein denotes Runx3
T11406 20945-20948 Protein denotes CD8
T11407 20924-20934 Gene_expression denotes expression
T11408 20924-20934 Gene_expression denotes expression
T11409 20890-20896 Positive_regulation denotes defect
T11410 20890-20896 Positive_regulation denotes defect
T11411 21040-21043 Protein denotes TCR
T11412 21055-21060 Protein denotes Runx3
T11413 21064-21067 Protein denotes CD8
T11414 21173-21177 Protein denotes CD8+
T11415 21214-21219 Protein denotes Runx3
T11416 21340-21359 Protein denotes perforin/granzyme B
T11417 21386-21391 Protein denotes Runx3
T11418 21395-21399 Protein denotes CD8+
T11419 21554-21557 Protein denotes Fas
T11420 21558-21561 Protein denotes Fas
T11421 21322-21332 Positive_regulation denotes activation
T11422 21373-21382 Negative_regulation denotes defective
T13673 21579-21584 Protein denotes Runx3
T13674 21733-21738 Protein denotes Runx3
T13675 21865-21870 Protein denotes Runx3
T13676 21891-21895 Protein denotes CD8+
T13677 21874-21881 Gene_expression denotes present
T13678 21941-21946 Protein denotes Runx1
T13679 21991-21996 Protein denotes Eomes
T13680 21998-22008 Protein denotes granzyme B
T13681 22010-22018 Protein denotes perforin
T13682 22024-22029 Protein denotes IFN-γ
T13683 21931-21940 Negative_regulation denotes represses
T13684 21978-21987 Positive_regulation denotes induction
T13685 21978-21987 Positive_regulation denotes induction
T13686 21978-21987 Positive_regulation denotes induction
T13687 21978-21987 Positive_regulation denotes induction
T13688 22031-22036 Protein denotes Runx3
T13689 22046-22055 Entity denotes promoters
T13690 22037-22042 Binding denotes binds
T13691 22227-22232 Protein denotes Runx1
T13692 22265-22270 Protein denotes Runx3
T13693 22286-22291 Protein denotes Runx3
T13694 22333-22336 Protein denotes TNF
T13695 22338-22342 Protein denotes IL-2
T13696 22348-22353 Protein denotes IFN-γ
T13697 22319-22329 Gene_expression denotes expression
T13698 22319-22329 Gene_expression denotes expression
T13699 22319-22329 Gene_expression denotes expression
T13700 22292-22303 Positive_regulation denotes contributed
T13701 22292-22303 Positive_regulation denotes contributed
T13702 22292-22303 Positive_regulation denotes contributed
T13703 22378-22381 Protein denotes TNF
T13704 22386-22390 Protein denotes IL-2
T13705 22412-22417 Protein denotes Runx3
T13706 22484-22489 Protein denotes Runx1
T13707 22515-22520 Protein denotes Runx3
T13708 22418-22426 Negative_regulation denotes subsides
T13709 22500-22511 Negative_regulation denotes derepressed
T13710 22542-22547 Protein denotes Runx3
T13711 22577-22582 Protein denotes IFN-γ
T13712 22583-22593 Gene_expression denotes expression
T13713 22564-22572 Positive_regulation denotes required
T13714 22820-22824 Protein denotes CD8+
T13715 23001-23005 Protein denotes CD8+
T13716 23040-23045 Protein denotes IFN-γ
T13717 23032-23039 Gene_expression denotes produce
T13718 22965-22971 Positive_regulation denotes needed
T13719 23174-23179 Protein denotes Runx3
T13720 23243-23248 Protein denotes IFN-γ
T13721 23229-23239 Gene_expression denotes expression
T13722 23209-23218 Positive_regulation denotes promoting
T13723 23318-23326 Protein denotes perforin
T13724 23377-23387 Protein denotes granzyme B
T13725 23475-23502 Protein denotes T-box transcription factors
T13726 23327-23337 Gene_expression denotes expression
T13727 23369-23376 Positive_regulation denotes induces
T13728 23304-23314 Positive_regulation denotes contribute
T13729 23655-23659 Protein denotes Prf1
T13730 23664-23668 Protein denotes Gzmb
T13731 23670-23673 Protein denotes Fig
T13732 23613-23619 Positive_regulation denotes induce
T13733 23613-23619 Positive_regulation denotes induce
T13734 23613-23619 Positive_regulation denotes induce
T13735 23810-23820 Protein denotes Eomes mRNA
T13736 23867-23871 Protein denotes Gzmb
T13737 23876-23885 Protein denotes Prf1 mRNA
T13738 23783-23796 Positive_regulation denotes up-regulation
T13739 23850-23863 Positive_regulation denotes up-regulation
T13740 23850-23863 Positive_regulation denotes up-regulation
T13741 23934-23942 Protein denotes perforin
T13742 23988-23995 Protein denotes Eo-VP16
T13743 24011-24021 Protein denotes granzyme B
T13744 24063-24068 Protein denotes Runx3
T13745 23943-23953 Gene_expression denotes expression
T13746 24022-24032 Gene_expression denotes expression
T13747 23926-23930 Regulation denotes role
T13748 24004-24010 Regulation denotes affect
T13749 24315-24320 Protein denotes Runx3
T13750 24342-24345 Protein denotes CD8
T13751 24402-24407 Protein denotes Runx3
T13752 24418-24426 Protein denotes perforin
T13753 24427-24437 Gene_expression denotes expression
T13754 24411-24417 Positive_regulation denotes induce
T13755 24529-24534 Protein denotes Runx3
T13756 24563-24582 Protein denotes T-box factors T-bet
T13757 24587-24592 Protein denotes Eomes
T13758 24597-24603 Protein denotes IL-2Rβ
T13759 24555-24562 Positive_regulation denotes induced
T13760 24555-24562 Positive_regulation denotes induced
T13761 24743-24748 Protein denotes Runx3
T13762 24781-24784 Protein denotes CD4
T13763 24889-24892 Protein denotes TCR
T13764 24905-24910 Protein denotes IFN-γ
T13765 24971-24975 Protein denotes CD8+
T13766 24997-25002 Protein denotes Runx3
T13767 25029-25061 Protein denotes T-box transcription factor Eomes
T13768 25018-25024 Positive_regulation denotes induce
T13769 25183-25194 Protein denotes Runx family
T14330 25423-25432 Protein denotes anti–IL-2
T14331 25434-25444 Protein denotes anti–IFN-γ
T14332 25446-25454 Protein denotes anti-TNF
T14333 25456-25471 Protein denotes anti–granzyme B
T14334 25473-25481 Protein denotes anti-CD8
T14335 25483-25492 Protein denotes anti-CD25
T14336 25503-25507 Protein denotes CD44
T14337 25509-25518 Protein denotes Anti-CD69
T14338 25620-25639 Protein denotes anti-Runx3 antibody
T14339 25644-25652 Gene_expression denotes produced
T14340 25830-25844 Protein denotes T-bet antibody
T14341 26044-26047 Protein denotes CD8
T14342 26085-26088 Protein denotes CD8
T14343 26178-26182 Protein denotes Gp33
T14893 26343-26347 Protein denotes CD8+
T14894 26357-26360 Protein denotes CD8
T14895 26396-26403 Protein denotes P14 TCR
T14896 26442-26447 Protein denotes Tbx21
T14897 26585-26589 Protein denotes CD8+
T14898 26603-26608 Protein denotes Runx3
T14899 26812-26826 Protein denotes Immune Disease
T14900 26926-26930 Protein denotes CD8+
T14901 27019-27027 Protein denotes anti-CD3
T14902 27029-27039 Protein denotes clone 2C11
T14903 27050-27054 Protein denotes CD28
T14904 27105-27121 Protein denotes anti–hamster IgG
T14905 27163-27166 Protein denotes TCR
T14906 27406-27412 Protein denotes rhIL-2
T16058 27428-27432 Protein denotes CD8+
T16059 27446-27451 Protein denotes Runx3
T16060 27461-27466 Protein denotes Runx3
T16061 27501-27504 Protein denotes CD4
T16062 27467-27476 Negative_regulation denotes deficient
T16063 27505-27515 Gene_expression denotes expression
T16064 27493-27500 Negative_regulation denotes silence
T16065 27603-27607 Protein denotes CD8+
T16066 27621-27629 Protein denotes Runx3 KO
T16067 27640-27643 Protein denotes CD8
T16068 27644-27647 Protein denotes CD4
T16069 27649-27651 Protein denotes SP
T16070 27655-27658 Protein denotes CD8
T16071 27659-27662 Protein denotes CD4
T16072 27733-27741 Protein denotes Runx3 KO
T16073 27786-27789 Protein denotes CD8
T16074 27790-27793 Protein denotes CD4
T16075 27852-27855 Protein denotes CD8
T16076 27856-27859 Protein denotes CD4
T16077 27909-27917 Protein denotes anti-CD3
T16078 27919-27928 Protein denotes anti-CD28
T16079 27967-27970 Protein denotes TCR
T16080 28058-28061 Protein denotes TCR
T16081 28087-28092 Protein denotes Runx3
T16082 28096-28099 Protein denotes CD8
T16083 28148-28151 Protein denotes CD4
T16084 28152-28162 Gene_expression denotes expression
T16085 28196-28201 Protein denotes Runx3
T16086 28309-28313 Protein denotes CD25
T16087 28318-28322 Protein denotes CD69
T16088 28324-28327 Protein denotes Fig
T16089 28329-28331 Protein denotes S1
T16090 28292-28305 Positive_regulation denotes up-regulation
T16091 28292-28305 Positive_regulation denotes up-regulation
T16092 28292-28305 Positive_regulation denotes up-regulation
T16093 28380-28384 Protein denotes CD25
T16094 28386-28391 Protein denotes Runx3
T16095 28395-28398 Protein denotes CD8
T16096 28421-28425 Protein denotes IL-2
T16097 28368-28379 Positive_regulation denotes up-regulate
T16098 28619-28622 Protein denotes CD4
T16099 28932-28937 Protein denotes Runx3
T16100 28941-28944 Protein denotes CD8
T16101 28974-28977 Protein denotes CD4
T16102 28623-28633 Gene_expression denotes expression
T16103 28964-28973 Gene_expression denotes coexpress
T16104 28610-28618 Negative_regulation denotes silenced
T16105 29046-29049 Protein denotes Fig
T16106 29033-29042 Gene_expression denotes presented
T16107 29051-29053 Protein denotes S2
T16108 29063-29071 Protein denotes Runx3 KO
T16109 29134-29139 Protein denotes Runx3
T16110 29140-29146 Protein denotes KO CD8
T16930 30388-30392 Protein denotes CD8+
T16931 30602-30605 Protein denotes TCR
T16932 30624-30627 Protein denotes CD8
T16933 30931-30936 Protein denotes Eomes
T16934 30937-30941 Protein denotes VP16
T17552 31782-31811 Protein denotes plus protease and phosphatase
T17553 32672-32679 Protein denotes RNase A
T17554 33363-33366 Protein denotes org
T17555 33367-33370 Protein denotes cgi
T18111 34389-34393 Protein denotes CD8+
T19043 6581-6585 Protein denotes CD8+
T19044 6626-6630 Protein denotes Prf1
T19045 6632-6636 Protein denotes Gzmb
T19046 6638-6643 Protein denotes Tbx21
T19047 6657-6667 Protein denotes Eomes mRNA
T19048 6698-6701 Protein denotes P14
T19049 6668-6678 Gene_expression denotes expression
T19050 6668-6678 Gene_expression denotes expression
T19051 6668-6678 Gene_expression denotes expression
T19052 6668-6678 Gene_expression denotes expression
T19053 6961-6964 Protein denotes P14
T19054 7139-7149 Protein denotes granzyme B
T19055 7151-7156 Protein denotes IFN-γ
T19056 7162-7165 Protein denotes TNF
T19057 7126-7134 Gene_expression denotes staining
T19058 7126-7134 Gene_expression denotes staining
T19059 7126-7134 Gene_expression denotes staining
T19060 7357-7360 Protein denotes P14
T19061 7421-7436 Protein denotes μM Gp33 peptide
T19062 7528-7531 Protein denotes CD8
T20023 11898-11906 Protein denotes perforin
T20024 11908-11918 Protein denotes granzyme B
T20025 11924-11929 Protein denotes IFN-γ
T20026 11930-11940 Gene_expression denotes expression
T20027 11930-11940 Gene_expression denotes expression
T20028 11884-11894 Regulation denotes Regulation
T20029 11884-11894 Regulation denotes Regulation
T20030 11989-11994 Protein denotes IFN-γ
T20031 12009-12011 Protein denotes WT
T20032 12013-12018 Protein denotes Tbx21
T20033 11995-12005 Gene_expression denotes expression
T20034 11995-12005 Gene_expression denotes expression
T20035 11995-12005 Gene_expression denotes expression
T20036 12069-12073 Protein denotes CD8+
T20037 12183-12188 Protein denotes IFN-γ
T20038 12189-12199 Gene_expression denotes expression
T20039 12271-12276 Protein denotes IFN-γ
T20040 12315-12319 Protein denotes Prf1
T20041 12324-12333 Protein denotes GzmB mRNA
T20042 12370-12373 Protein denotes CD8
T20043 12477-12481 Protein denotes VP16
T20044 12483-12490 Protein denotes Eo-VP16
T20045 12334-12344 Gene_expression denotes expression
T20046 12334-12344 Gene_expression denotes expression
T20047 12460-12470 Gene_expression denotes expressing
T20048 12460-12470 Gene_expression denotes expressing
T20049 12707-12717 Protein denotes Granzyme B
T20050 12722-12727 Protein denotes IFN-γ
T20051 12742-12747 Protein denotes Tbx21
T20052 12755-12760 Protein denotes Tbx21
T20053 12728-12738 Gene_expression denotes expression
T20054 12728-12738 Gene_expression denotes expression
T20055 12839-12844 Protein denotes IFN-γ
T20056 12881-12888 Protein denotes Eo-VP16
T20057 12845-12855 Gene_expression denotes production
T20058 13024-13034 Protein denotes GFP+ IFN-γ
T20707 15071-15076 Protein denotes Runx3
T20708 15134-15139 Protein denotes Runx3
T20709 15159-15167 Protein denotes perforin
T20710 15182-15187 Protein denotes Runx3
T20711 15207-15210 Protein denotes CD8
T20712 15212-15216 Protein denotes SP T
T20713 15168-15178 Gene_expression denotes expression
T20714 15168-15178 Gene_expression denotes expression
T20715 15249-15254 Protein denotes Actin
T20716 15316-15325 Protein denotes Prf1 mRNA
T20717 15340-15345 Protein denotes Runx3
T20718 15365-15368 Protein denotes CD8
T20719 15326-15336 Gene_expression denotes expression
T20720 15404-15409 Protein denotes Actin
T20721 15459-15469 Protein denotes granzyme B
T20722 15471-15476 Protein denotes IFN-γ
T20723 15478-15481 Protein denotes TNF
T20724 15487-15491 Protein denotes IL-2
T20725 15525-15530 Protein denotes Runx3
T20726 15550-15553 Protein denotes CD8
T20727 15555-15559 Protein denotes SP T
T20728 15445-15455 Gene_expression denotes Expression
T20729 15445-15455 Gene_expression denotes Expression
T20730 15445-15455 Gene_expression denotes Expression
T20731 15445-15455 Gene_expression denotes Expression
T20732 15646-15652 Protein denotes WT GFP
T20733 15759-15775 Protein denotes endogenous Runx3
T20734 15780-15785 Protein denotes Eomes
T20735 15793-15803 Protein denotes Prf1 locus
T20736 15748-15755 Binding denotes binding
T20737 15748-15755 Binding denotes binding
T20738 15748-15755 Binding denotes binding
T20739 15748-15755 Binding denotes binding
T20740 16034-16038 Protein denotes CD8+
T20741 16124-16128 Protein denotes Prf1
T20742 16147-16157 Protein denotes Runx3 ChIP
T20743 16175-16185 Protein denotes Eomes ChIP
T22048 19206-19211 Protein denotes Runx3
T22049 19228-19236 Protein denotes perforin
T22050 19238-19248 Protein denotes granzyme B
T22051 19254-19259 Protein denotes IFN-γ
T22052 19212-19220 Regulation denotes controls
T22053 19212-19220 Regulation denotes controls
T22054 19260-19270 Gene_expression denotes expression
T22055 19260-19270 Gene_expression denotes expression
T22056 19260-19270 Gene_expression denotes expression
T22057 19212-19220 Regulation denotes controls
T22058 19212-19220 Regulation denotes controls
T22059 19212-19220 Regulation denotes controls
T22060 19289-19294 Protein denotes Runx3
T22061 19301-19306 Protein denotes Runx3
T22062 19310-19313 Protein denotes CD8
T22063 19388-19405 Protein denotes IRES-GFP cassette
T22064 19407-19410 Protein denotes GFP
T22065 19429-19439 Protein denotes Eomes-VP16
T22066 19441-19443 Protein denotes Eo
T22067 19444-19448 Protein denotes VP16
T22068 19453-19456 Protein denotes Myc
T22069 19457-19462 Protein denotes Runx3
T22070 19464-19469 Protein denotes Runx3
T22071 19696-19701 Protein denotes Eomes
T22072 19702-19706 Protein denotes VP16
T22073 19830-19857 Protein denotes VP16 transactivation domain
T22074 19678-19692 Positive_regulation denotes Overexpression
T22075 19678-19692 Positive_regulation denotes Overexpression
T22076 19877-19887 Protein denotes granzyme B
T22077 19892-19897 Protein denotes IFN-γ
T22078 19863-19873 Gene_expression denotes Expression
T22079 19863-19873 Gene_expression denotes Expression
T22080 20120-20130 Protein denotes granzyme B
T22081 20131-20139 Gene_expression denotes staining
T22082 20235-20241 Protein denotes WT GFP
T22083 20381-20386 Protein denotes Runx3
T22084 20391-20404 Protein denotes T-box factors
T22085 20426-20429 Protein denotes TCR
T22086 20465-20470 Protein denotes IFN-γ
T22087 20471-20481 Gene_expression denotes expression
T22088 20445-20454 Positive_regulation denotes essential
T22089 20483-20488 Protein denotes Runx3
T22090 20509-20513 Protein denotes CD8+
T22091 20536-20541 Protein denotes Runx1
T22092 20554-20559 Protein denotes Eomes
T22093 20561-20569 Protein denotes perforin
T22094 20571-20581 Protein denotes granzyme B
T22095 20587-20592 Protein denotes IFN-γ
T22096 20492-20499 Gene_expression denotes present
T22097 20526-20535 Negative_regulation denotes represses
T22098 20593-20603 Gene_expression denotes expression
T22099 20593-20603 Gene_expression denotes expression
T22100 20646-20651 Protein denotes IFN-γ
T22101 20672-20677 Protein denotes Runx3
T22102 20730-20738 Protein denotes perforin
T22103 20652-20662 Gene_expression denotes expression
T22104 20739-20749 Gene_expression denotes expression
T22105 20615-20626 Regulation denotes participate
T22106 20721-20729 Positive_regulation denotes activate
T22107 20819-20828 Protein denotes Gzmb mRNA
T22108 20837-20855 Protein denotes granzyme B protein
T22109 20856-20866 Gene_expression denotes expression
T22110 20856-20866 Gene_expression denotes expression
T22111 20789-20795 Regulation denotes effect
T22112 20789-20795 Regulation denotes effect
T16367 29211-29214 Protein denotes EL4
T16368 29260-29275 Protein denotes μM Gp33 peptide
T16369 29315-29318 Protein denotes P14
T16370 29501-29504 Protein denotes CD8
T16371 29505-29520 Protein denotes allophycocyanin
T16372 29608-29611 Protein denotes CD8
T18274 35053-35056 Protein denotes Fig
T18275 35058-35060 Protein denotes S1
T18276 35102-35106 Protein denotes CD8+
T18277 35120-35125 Protein denotes Runx3
T18278 35135-35138 Protein denotes Fig
T18279 35180-35188 Protein denotes Runx3 WT
T18280 35288-35296 Protein denotes Table S1
T18281 35358-35361 Protein denotes org
T18282 35362-35365 Protein denotes cgi
T633 897-905 Protein denotes perforin
T634 911-921 Protein denotes granzyme B
T635 824-834 Gene_expression denotes expression
T636 824-834 Gene_expression denotes expression
T637 824-834 Gene_expression denotes expression
T638 786-795 Regulation denotes regulates
T639 786-795 Regulation denotes regulates
T640 786-795 Regulation denotes regulates
T641 1004-1009 Protein denotes Runx3
T642 1053-1080 Protein denotes T-box transcription factors
R3581 T4567 T4568 themeOf see,increased
R3582 T4532 T4534 themeOf perforin,expression
R3583 T4533 T4535 themeOf granzyme B,expression
R3584 T4537 T4539 themeOf perforin mRNA,expression
R3585 T4570 T4571 themeOf perforin,express
R3586 T4538 T4540 themeOf perforin,expression
R3587 T4541 T4543 themeOf perforin,expression
R3588 T4542 T4544 themeOf Prf1,expression
R3589 T4573 T4576 themeOf granzyme B,expression
R3590 T4543 T4545 themeOf expression,increase
R3591 T4543 T4547 themeOf expression,decrease
R3592 T4543 T4549 themeOf expression,reexpression
R3593 T4544 T4546 themeOf expression,increase
R3594 T4544 T4548 themeOf expression,decrease
R3595 T4544 T4550 themeOf expression,reexpression
R3596 T4574 T4577 themeOf perforin,expression
R3597 T4551 T4554 themeOf granzyme B,undetectable
R3598 T4551 T4556 themeOf granzyme B,up-regulated
R3599 T4551 T4558 themeOf granzyme B,increased
R3600 T4577 T4578 themeOf expression,induced
R3601 T4552 T4555 themeOf Gzmb,undetectable
R3602 T4552 T4557 themeOf Gzmb,up-regulated
R3603 T4552 T4559 themeOf Gzmb,increased
R3604 T4553 T4560 themeOf granzyme B protein,expressed
R3605 T4553 T4561 themeOf granzyme B protein,high
R3606 T4562 T4565 themeOf IFN-γ,expressed
R3607 T4563 T4566 themeOf TNF,expressed
R3608 T4564 T4567 themeOf Fig. 2 A,see
R4666 T5866 T5868 themeOf Prf1,regulation
R4667 T5867 T5869 themeOf GzmB,regulation
R4668 T5873 T5874 themeOf TCR,expression
R4669 T5874 T5875 themeOf expression,effect
R4670 T5876 T5877 themeOf Eomes mRNA,induction
R4671 T5878 T5881 themeOf GzmB mRNA,expression
R4672 T5879 T5882 themeOf Eomes mRNA,expressed
R4673 T5880 T5883 themeOf perforin mRNA,reexpression
R4674 T5881 T5884 themeOf expression,preceded
R4675 T5885 T5887 themeOf IFN-γ,production
R4676 T5886 T5888 themeOf perforin,expression
R4677 T5887 T5889 themeOf production,required
R4678 T5888 T5890 themeOf expression,control
R4679 T5890 T5891 themeOf control,contribute
R4680 T5892 T5896 themeOf TCR,induce
R4681 T5893 T5897 themeOf IFN-γ,induces
R4682 T5895 T5898 themeOf perforin,expression
R4683 T5898 T5899 themeOf expression,activates
R5756 T7318 T7320 themeOf Perforin,expression
R5757 T7318 T7322 themeOf Perforin,regulated
R5758 T7319 T7321 themeOf granzyme B,expression
R5759 T7320 T7323 themeOf expression,regulated
R5760 T7321 T7324 themeOf expression,regulated
R5761 T7325 T7331 themeOf IFN-γ,expression
R5762 T7326 T7332 themeOf perforin,expression
R5763 T7327 T7333 themeOf granzyme B,expression
R5764 T7329 T7334 themeOf WT,-deficient
R5765 T7330 T7335 themeOf Tbx21,-deficient
R5766 T7337 T7338 themeOf IFN-γ,produced
R5767 T7340 T7341 themeOf perforin mRNA,expression
R5768 T7341 T7342 themeOf expression,defect
R5769 T7343 T7346 themeOf GzmB mRNA,reduction
R5770 T7344 T7347 themeOf granzyme B protein,expression
R5771 T7345 T7348 themeOf Fig,expression
R5772 T7347 T7349 themeOf expression,decrease
R5773 T7348 T7350 themeOf expression,decrease
R5774 T7355 T7359 causeOf VP16,increased
R5775 T7356 T7358 themeOf perforin,expression
R5776 T7358 T7359 themeOf expression,increased
R5777 T7360 T7364 causeOf Eo-VP16,defect
R5778 T7361 T7363 themeOf IFN-γ,production
R5779 T7363 T7364 themeOf production,defect
R5780 T7365 T7371 causeOf Eo-VP16,induce
R5781 T7366 T7369 themeOf GzmB mRNA,expression
R5782 T7367 T7370 themeOf GzmB mRNA,expression
R5783 T7369 T7371 themeOf expression,induce
R8797 T11240 T11245 themeOf Runx3,expressed
R8802 T11254 T11258 themeOf perforin,expression
R8804 T11255 T11259 themeOf granzyme B,expression
R8805 T11256 T11260 themeOf IFN-γ,expression
R8806 T11257 T11261 themeOf Fig,expression
R8809 T11262 T11264 themeOf perforin mRNA,expression
R8810 T11262 T11265 causeOf perforin mRNA,undetectable
R8812 T11264 T11265 themeOf expression,undetectable
R8817 T11267 T11275 causeOf Runx3,required
R8818 T11267 T11276 causeOf Runx3,required
R8819 T11267 T11277 causeOf Runx3,required
R8820 T11268 T11272 themeOf IFN-γ,production
R8821 T11269 T11273 themeOf TNF,production
R8822 T11270 T11274 themeOf IL-2,production
R8823 T11272 T11275 themeOf production,required
R8824 T11273 T11276 themeOf production,required
R8826 T11274 T11277 themeOf production,required
R8828 T11278 T11281 themeOf Runx3,induces
R8833 T11283 T11290 themeOf perforin,expression
R8834 T11284 T11291 themeOf Eo,overexpression
R8835 T11285 T11292 themeOf VP16,overexpression
R8836 T11286 T11293 themeOf perforin,increase
R8837 T11286 T11294 themeOf perforin,expression
R8843 T11287 T11295 themeOf IFN-γ,expression
R8845 T11288 T11296 themeOf Runx,involving
R8846 T11289 T11297 themeOf T-box transcription factors,involving
R8847 T11291 T11298 causeOf overexpression,led
R8848 T11292 T11299 causeOf overexpression,led
R8849 T11293 T11298 themeOf increase,led
R8850 T11293 T11299 themeOf increase,led
R8851 T11294 T11300 themeOf expression,increase
R8852 T11295 T11301 themeOf expression,increase
R8855 T11302 T11309 causeOf Runx3,regulate
R8856 T11302 T11310 causeOf Runx3,regulate
R8857 T11302 T11311 causeOf Runx3,regulate
R8858 T11303 T11306 themeOf perforin,transcription
R8860 T11304 T11307 themeOf IFN-γ,transcription
R8862 T11305 T11308 themeOf granzyme B,transcription
R8863 T11306 T11309 themeOf transcription,regulate
R8864 T11307 T11310 themeOf transcription,regulate
R8866 T11308 T11311 themeOf transcription,regulate
R8868 T11312 T11319 themeOf Eomes,bound
R8869 T11312 T11320 themeOf Eomes,bound
R8870 T11312 T11321 themeOf Eomes,bound
R8871 T11313 T11322 themeOf Runx3,bound
R8872 T11313 T11323 themeOf Runx3,bound
R8873 T11313 T11324 themeOf Runx3,bound
R8874 T11314 T11319 themeOf Prf1,bound
R8875 T11314 T11322 themeOf Prf1,bound
R8876 T11314 T11318 partOf Prf1,regions
R8877 T11315 T11320 themeOf Ifng,bound
R8878 T11315 T11323 themeOf Ifng,bound
R8879 T11315 T11318 partOf Ifng,regions
R8880 T11316 T11318 partOf Gzmb genes,regions
R8881 T11317 T11321 themeOf Fig,bound
R8882 T11317 T11324 themeOf Fig,bound
R8883 T11317 T11318 partOf Fig,regions
R8884 T11318 T11319 Site regions,bound
R8885 T11318 T11320 Site regions,bound
R8886 T11318 T11321 Site regions,bound
R8887 T11318 T11322 Site regions,bound
R8888 T11318 T11323 Site regions,bound
R8889 T11318 T11324 Site regions,bound
R8891 T11325 T11333 themeOf Runx3,bound
R8894 T11351 T11354 themeOf expression,controlled
R8895 T11331 T11333 themeOf Ifng promoter,bound
R8896 T11334 T11344 themeOf Prf1 TSS,bound
R8897 T11334 T11340 partOf Prf1 TSS,enhancer
R8898 T11335 T11342 themeOf Il2rb gene,target
R8899 T11335 T11341 partOf Il2rb gene,promoter
R8900 T11336 T11343 themeOf Ifng TSS,target
R8901 T11337 T11343 themeOf T-box proteins,target
R8902 T11340 T11344 Site enhancer,bound
R8903 T11341 T11342 Site promoter,target
R8904 T11367 T11371 themeOf perforin,restored
R8905 T11345 T11354 causeOf Runx3,controlled
R8906 T11346 T11351 themeOf CTL effector genes,expression
R8907 T11347 T11352 themeOf Runx3,expressed
R8908 T11348 T11353 themeOf Eo-VP16,expressed
R8909 T11367 T11372 themeOf perforin,expression
R8910 T11368 T11373 themeOf granzyme B,expression
R8911 T11369 T11374 themeOf IFN-γ,expression
R8912 T11370 T11375 themeOf Fig,expression
R8913 T11372 T11376 themeOf expression,restored
R8914 T11373 T11377 themeOf expression,restored
R8915 T11374 T11378 themeOf expression,restored
R8916 T11375 T11379 themeOf expression,restored
R8917 T11381 T11385 themeOf Runx1,up-regulation
R8918 T11382 T11386 causeOf Runx3,suppressed
R8919 T11383 T11387 themeOf Runx1,repression
R8920 T11384 T11387 causeOf Runx3,repression
R8921 T11385 T11386 themeOf up-regulation,suppressed
R8922 T11388 T11396 causeOf Eo-VP16,up-regulate
R8923 T11389 T11393 themeOf perforin,expression
R8924 T11391 T11394 themeOf IFN-γ,expression
R8925 T11392 T11395 themeOf TCR,restimulation
R8926 T11393 T11396 themeOf expression,up-regulate
R8927 T11394 T11397 themeOf expression,induce
R8928 T11395 T11397 causeOf restimulation,induce
R8929 T11397 T11398 themeOf induce,restored
R8930 T11399 T11402 themeOf perforin,expression
R8931 T11403 T11407 themeOf perforin,expression
R8932 T11404 T11408 themeOf granzyme B,expression
R8933 T11407 T11409 themeOf expression,defect
R8934 T11408 T11410 themeOf expression,defect
R8935 T11416 T11421 themeOf perforin/granzyme B,activation
R8936 T11421 T11422 themeOf activation,defective
R10737 T13675 T13677 themeOf Runx3,present
R10738 T13678 T13683 themeOf Runx1,represses
R10739 T13679 T13684 themeOf Eomes,induction
R10740 T13680 T13685 themeOf granzyme B,induction
R10741 T13681 T13686 themeOf perforin,induction
R10742 T13682 T13687 themeOf IFN-γ,induction
R10743 T13688 T13690 themeOf Runx3,binds
R10744 T13689 T13690 Site promoters,binds
R10745 T13693 T13700 causeOf Runx3,contributed
R10746 T13693 T13701 causeOf Runx3,contributed
R10747 T13693 T13702 causeOf Runx3,contributed
R10748 T13694 T13697 themeOf TNF,expression
R10749 T13695 T13698 themeOf IL-2,expression
R10750 T13696 T13699 themeOf IFN-γ,expression
R10751 T13697 T13700 themeOf expression,contributed
R10752 T13698 T13701 themeOf expression,contributed
R10753 T13699 T13702 themeOf expression,contributed
R10754 T13705 T13708 themeOf Runx3,subsides
R10755 T13706 T13709 themeOf Runx1,derepressed
R10756 T13710 T13713 causeOf Runx3,required
R10757 T13711 T13712 themeOf IFN-γ,expression
R10758 T13712 T13713 themeOf expression,required
R10759 T13716 T13717 themeOf IFN-γ,produce
R10760 T13717 T13718 themeOf produce,needed
R10761 T13720 T13721 themeOf IFN-γ,expression
R10762 T13721 T13722 themeOf expression,promoting
R10763 T13723 T13726 themeOf perforin,expression
R10764 T13724 T13727 themeOf granzyme B,induces
R10765 T13726 T13728 themeOf expression,contribute
R10766 T13729 T13732 themeOf Prf1,induce
R10767 T13730 T13733 themeOf Gzmb,induce
R10768 T13731 T13734 themeOf Fig,induce
R10769 T13735 T13738 themeOf Eomes mRNA,up-regulation
R10770 T13736 T13739 themeOf Gzmb,up-regulation
R10771 T13737 T13740 themeOf Prf1 mRNA,up-regulation
R10772 T13741 T13745 themeOf perforin,expression
R11154 T14338 T14339 themeOf anti-Runx3 antibody,produced
R12339 T16060 T16062 themeOf Runx3,deficient
R12340 T16061 T16063 themeOf CD4,expression
R12341 T16062 T16064 causeOf deficient,silence
R12342 T16063 T16064 themeOf expression,silence
R12343 T16083 T16084 themeOf CD4,expression
R12344 T16086 T16090 themeOf CD25,up-regulation
R12345 T16087 T16091 themeOf CD69,up-regulation
R12346 T16089 T16092 themeOf S1,up-regulation
R12347 T16093 T16097 themeOf CD25,up-regulate
R12348 T16098 T16102 themeOf CD4,expression
R12349 T16101 T16103 themeOf CD4,coexpress
R12350 T16102 T16104 themeOf expression,silenced
R12351 T16105 T16106 themeOf Fig,presented
R15500 T20023 T20026 themeOf perforin,expression
R15501 T20025 T20027 themeOf IFN-γ,expression
R15502 T20026 T20028 themeOf expression,Regulation
R15503 T20027 T20029 themeOf expression,Regulation
R15504 T20030 T20033 themeOf IFN-γ,expression
R15505 T20031 T20034 themeOf WT,expression
R15506 T20032 T20035 themeOf Tbx21,expression
R15507 T20037 T20038 themeOf IFN-γ,expression
R15508 T20040 T20045 themeOf Prf1,expression
R15509 T20041 T20046 themeOf GzmB mRNA,expression
R15510 T20043 T20047 themeOf VP16,expressing
R15511 T20044 T20048 themeOf Eo-VP16,expressing
R15512 T20049 T20053 themeOf Granzyme B,expression
R15513 T20050 T20054 themeOf IFN-γ,expression
R15514 T20055 T20057 themeOf IFN-γ,production
R15867 T20708 T20713 themeOf Runx3,expression
R15868 T20709 T20714 themeOf perforin,expression
R15869 T20716 T20719 themeOf Prf1 mRNA,expression
R15870 T20721 T20728 themeOf granzyme B,Expression
R15871 T20722 T20729 themeOf IFN-γ,Expression
R15872 T20723 T20730 themeOf TNF,Expression
R15873 T20724 T20731 themeOf IL-2,Expression
R15874 T20733 T20736 themeOf endogenous Runx3,binding
R15875 T20733 T20738 themeOf endogenous Runx3,binding
R15876 T20734 T20737 themeOf Eomes,binding
R15877 T20734 T20739 themeOf Eomes,binding
R15878 T20735 T20738 themeOf Prf1 locus,binding
R15879 T20735 T20739 themeOf Prf1 locus,binding
R16902 T22048 T22052 causeOf Runx3,controls
R16903 T22048 T22053 causeOf Runx3,controls
R16904 T22048 T22057 causeOf Runx3,controls
R16905 T22048 T22058 causeOf Runx3,controls
R16906 T22048 T22059 causeOf Runx3,controls
R16907 T22049 T22052 themeOf perforin,controls
R16908 T22049 T22054 themeOf perforin,expression
R16909 T22050 T22053 themeOf granzyme B,controls
R16910 T22050 T22055 themeOf granzyme B,expression
R16911 T22051 T22056 themeOf IFN-γ,expression
R16912 T22054 T22057 themeOf expression,controls
R16913 T22055 T22058 themeOf expression,controls
R16914 T22056 T22059 themeOf expression,controls
R16915 T22071 T22074 themeOf Eomes,Overexpression
R16916 T22072 T22075 themeOf VP16,Overexpression
R16917 T22076 T22078 themeOf granzyme B,Expression
R16918 T22077 T22079 themeOf IFN-γ,Expression
R16919 T22080 T22081 themeOf granzyme B,staining
R16920 T22086 T22087 themeOf IFN-γ,expression
R16921 T22087 T22088 themeOf expression,essential
R16922 T22089 T22096 themeOf Runx3,present
R16923 T22089 T22097 causeOf Runx3,represses
R16924 T22091 T22097 themeOf Runx1,represses
R16925 T22092 T22098 themeOf Eomes,expression
R16926 T22095 T22099 themeOf IFN-γ,expression
R16927 T22100 T22103 themeOf IFN-γ,expression
R16928 T22101 T22106 causeOf Runx3,activate
R16929 T22102 T22104 themeOf perforin,expression
R16930 T22103 T22105 themeOf expression,participate
R16931 T22104 T22106 themeOf expression,activate
R16932 T22107 T22109 themeOf Gzmb mRNA,expression
R16933 T22107 T22111 causeOf Gzmb mRNA,effect
R16934 T22107 T22112 causeOf Gzmb mRNA,effect
R16935 T22108 T22110 themeOf granzyme B protein,expression
R16936 T22109 T22111 themeOf expression,effect
R16937 T22110 T22112 themeOf expression,effect
R429 T631 T638 causeOf Runx3,regulates
R430 T631 T639 causeOf Runx3,regulates
R431 T631 T640 causeOf Runx3,regulates
R432 T632 T635 themeOf IFN-γ,expression
R527 T633 T636 themeOf perforin,expression
R528 T634 T637 themeOf granzyme B,expression
R529 T635 T638 themeOf expression,regulates
R530 T636 T639 themeOf expression,regulates
R531 T637 T640 themeOf expression,regulates
R10773 T13742 T13748 causeOf Eo-VP16,affect
R10774 T13743 T13746 themeOf granzyme B,expression
R10775 T13745 T13747 themeOf expression,role
R10776 T13746 T13748 themeOf expression,affect
R10777 T13751 T13754 causeOf Runx3,induce
R10778 T13752 T13753 themeOf perforin,expression
R10779 T13753 T13754 themeOf expression,induce
R10780 T13756 T13759 themeOf T-box factors T-bet,induced
R10781 T13757 T13760 themeOf Eomes,induced
R10782 T13766 T13768 causeOf Runx3,induce
R10783 T13767 T13768 themeOf T-box transcription factor Eomes,induce
R14783 T19044 T19049 themeOf Prf1,expression
R14784 T19045 T19050 themeOf Gzmb,expression
R14785 T19046 T19051 themeOf Tbx21,expression
R14786 T19047 T19052 themeOf Eomes mRNA,expression
R14787 T19054 T19057 themeOf granzyme B,staining
R14788 T19055 T19058 themeOf IFN-γ,staining
R14789 T19056 T19059 themeOf TNF,staining

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Negation Speculation
T1419 3745-3755 Gene_expression denotes expression
T1420 3782-3786 Binding denotes bind
T1421 3782-3786 Binding denotes bind
T1422 3828-3838 Gene_expression denotes expression
T1423 3842-3852 Regulation denotes unaffected true
T1424 3862-3871 Negative_regulation denotes deficient
T1425 1897-1905 Negative_regulation denotes deletion
T1426 3887-3894 Negative_regulation denotes lacking
T1427 3906-3915 Negative_regulation denotes decreased
T1428 3916-3926 Gene_expression denotes expression
T1429 3906-3915 Negative_regulation denotes decreased
T1430 3916-3926 Gene_expression denotes expression
T1431 3967-3972 Binding denotes binds
T1432 3967-3972 Binding denotes binds
T1433 4133-4142 Regulation denotes regulator
T1434 4151-4161 Gene_expression denotes expression
T1435 4216-4223 Positive_regulation denotes promote
T1436 1897-1905 Negative_regulation denotes deletion
T1437 4224-4237 Transcription denotes transcription
T1438 4216-4223 Positive_regulation denotes promote
T1439 4224-4237 Transcription denotes transcription
T1440 2059-2068 Negative_regulation denotes deficient
T1441 2059-2068 Negative_regulation denotes deficient
T1442 2560-2569 Binding denotes targeting
T1443 2560-2569 Binding denotes targeting
T1444 2790-2799 Transcription denotes expressed true
T1445 1144-1147 Protein denotes CD8
T1446 1929-1934 Protein denotes T-bet
T1447 1940-1952 Protein denotes eomesodermin
T1448 1998-2001 Protein denotes CD8
T1449 2078-2083 Protein denotes T-bet
T1450 2088-2093 Protein denotes Eomes
T1451 2300-2305 Protein denotes T-bet
T1452 2310-2315 Protein denotes Eomes
T1453 2496-2499 Protein denotes CD8
T1454 2728-2731 Protein denotes CD4
T1455 1287-1295 Protein denotes perforin
T1456 2732-2735 Protein denotes CD8
T1457 2762-2767 Protein denotes Runx3
T1458 2772-2780 Protein denotes perforin
T1459 2839-2842 Protein denotes CD8
T1460 2887-2890 Protein denotes CD4
T1461 2916-2921 Protein denotes Runx3
T1462 2948-2951 Protein denotes CD4
T1463 3030-3035 Protein denotes Runx3
T1464 3118-3121 Protein denotes Il4
T1465 3126-3130 Protein denotes Ifng
T1466 1357-1362 Protein denotes IFN-γ
T1467 3217-3220 Protein denotes CD8
T1468 3244-3249 Protein denotes Runx3
T1469 3260-3263 Protein denotes CD8
T1470 3343-3348 Protein denotes Runx3
T1471 3423-3428 Protein denotes Runx3
T1472 3506-3511 Protein denotes T-bet
T1473 3627-3632 Protein denotes Eomes
T1474 3686-3691 Protein denotes IFN-γ
T1475 3704-3709 Protein denotes Runx3
T1476 3726-3731 Protein denotes Eomes
T1477 1367-1370 Protein denotes TNF
T1478 3736-3744 Protein denotes perforin
T1479 3766-3771 Protein denotes Eomes
T1480 3776-3781 Protein denotes Runx3
T1481 3794-3798 Protein denotes Prf1
T1482 3819-3827 Protein denotes perforin
T1483 3856-3861 Protein denotes T-bet
T1484 3895-3900 Protein denotes Runx3
T1485 3930-3935 Protein denotes IFN-γ
T1486 3940-3950 Protein denotes granzyme B
T1487 3956-3961 Protein denotes Runx3
T1488 1626-1629 Protein denotes CD4
T1489 4001-4005 Protein denotes Ifng
T1490 4010-4014 Protein denotes Gzmb
T1491 4114-4119 Protein denotes Runx3
T1492 4146-4150 Protein denotes Gzmb
T1493 4182-4187 Protein denotes T-bet
T1494 4192-4197 Protein denotes Eomes
T1495 4245-4249 Protein denotes Ifng
T1496 4254-4258 Protein denotes Prf1
T1497 1756-1768 Protein denotes Eomesodermin
T1498 1770-1775 Protein denotes Eomes
T1499 1781-1786 Protein denotes T-bet
T1500 3977-3993 Entity denotes promoter regions
T1501 1913-1918 Protein denotes Tbx21
T1502 2019-2025 Protein denotes IL-2Rβ
T6101 13781-13786 Protein denotes T-bet
T6102 13787-13796 Negative_regulation denotes deficient
T6103 13822-13827 Protein denotes T-bet
T6104 13828-13838 Positive_regulation denotes dependence
T6105 13842-13846 Protein denotes GzmB
T6106 13847-13862 Transcription denotes mRNA expression
T6107 13892-13899 Protein denotes Eo-VP16
T7771 14629-14634 Protein denotes IFN-γ
T7772 14671-14679 Protein denotes perforin
T7773 14697-14707 Gene_expression denotes expression
T7774 14725-14737 Positive_regulation denotes undetectable true
T7775 14741-14746 Protein denotes Runx3
T7776 14797-14802 Protein denotes Runx3
T7777 14837-14842 Protein denotes Eomes
T7778 14843-14853 Gene_expression denotes expression true
T7779 14868-14873 Protein denotes T-bet
T7780 14874-14884 Gene_expression denotes expression
T7781 14889-14899 Negative_regulation denotes unimpaired true
T7782 14925-14930 Protein denotes Runx3
T7783 14935-14943 Positive_regulation denotes required
T7784 14935-14943 Positive_regulation denotes required
T7785 14935-14943 Positive_regulation denotes required
T7786 14956-14966 Gene_expression denotes production
T7787 14956-14966 Gene_expression denotes production
T7788 14956-14966 Gene_expression denotes production
T7789 14970-14975 Protein denotes IFN-γ
T7790 14985-14988 Protein denotes TNF
T7791 14992-14996 Protein denotes IL-2
T7792 15001-15004 Protein denotes CD8
T7793 16290-16295 Protein denotes T-bet
T7794 16299-16311 Positive_regulation denotes up-regulated
T7795 16322-16329 Positive_regulation denotes induces
T7796 16330-16335 Protein denotes Runx3
T7797 16349-16354 Protein denotes T-bet
T7798 16359-16364 Protein denotes Runx3
T7799 16378-16384 Positive_regulation denotes induce
T7800 16378-16384 Positive_regulation denotes induce
T7801 16385-16390 Protein denotes IFN-γ
T7802 16395-16402 Negative_regulation denotes silence
T7803 16395-16402 Negative_regulation denotes silence
T7804 16403-16407 Protein denotes IL-4
T7805 16492-16497 Protein denotes Runx3
T7806 16507-16516 Positive_regulation denotes necessary true
T7807 16521-16526 Protein denotes Eomes
T7808 16527-16536 Positive_regulation denotes induction
T7809 16569-16574 Protein denotes Eomes
T7810 16575-16585 Gene_expression denotes expression
T7811 16606-16614 Protein denotes perforin
T7812 16615-16625 Gene_expression denotes expression
T7813 16644-16658 Positive_regulation denotes overexpression
T7814 16662-16669 Protein denotes Eo-VP16
T7815 16686-16691 Protein denotes T-bet
T7816 16692-16701 Negative_regulation denotes deficient
T7817 16710-16713 Positive_regulation denotes led
T7818 16710-16713 Positive_regulation denotes led
T7819 16720-16728 Positive_regulation denotes increase
T7820 16720-16728 Positive_regulation denotes increase
T7821 16737-16745 Protein denotes perforin
T7822 16750-16755 Protein denotes IFN-γ
T7823 16756-16766 Gene_expression denotes expression
T7824 16756-16766 Gene_expression denotes expression
T7825 16982-16987 Protein denotes Runx3
T7826 16999-17008 Positive_regulation denotes necessary true
T7827 17013-17018 Protein denotes Eomes
T7828 17019-17028 Positive_regulation denotes induction
T7829 17051-17056 Protein denotes Eomes
T7830 17060-17068 Regulation denotes regulate
T7831 17060-17068 Regulation denotes regulate
T7832 17060-17068 Regulation denotes regulate
T7833 17060-17068 Regulation denotes regulate
T7834 17060-17068 Regulation denotes regulate
T7835 17060-17068 Regulation denotes regulate
T7836 17069-17082 Transcription denotes transcription
T7837 17069-17082 Transcription denotes transcription
T7838 17069-17082 Transcription denotes transcription
T7839 17111-17119 Protein denotes perforin
T7840 17121-17126 Protein denotes IFN-γ
T7841 17132-17142 Protein denotes granzyme B
T7842 17236-17241 Protein denotes Eomes
T7843 17246-17251 Protein denotes Runx3
T7844 17252-17257 Binding denotes bound true
T7845 17252-17257 Binding denotes bound true
T7846 17252-17257 Binding denotes bound true
T7847 17252-17257 Binding denotes bound true
T7848 17252-17257 Binding denotes bound true
T7849 17252-17257 Binding denotes bound true
T7850 17258-17276 Entity denotes regulatory regions
T7851 17284-17288 Protein denotes Prf1
T7852 17290-17294 Protein denotes Ifng
T7853 17300-17304 Protein denotes Gzmb
T7854 17337-17347 Binding denotes associated
T7855 17337-17347 Binding denotes associated
T7856 17401-17406 Protein denotes Runx3
T7857 17407-17412 Binding denotes bound
T7858 17407-17412 Binding denotes bound
T7859 17420-17424 Protein denotes Prf1
T7860 17429-17433 Protein denotes Gzmb
T7861 17434-17459 Entity denotes transcription start sites
T7862 17529-17533 Protein denotes Prf1
T7863 17609-17613 Protein denotes Prf1
T7864 17633-17637 Protein denotes Ifng
T7865 17755-17759 Protein denotes Ifng
T7866 17800-17805 Protein denotes Eomes
T7867 17806-17811 Binding denotes bound
T7868 17806-17811 Binding denotes bound
T7869 17829-17833 Protein denotes Prf1
T7870 17834-17837 Entity denotes TSS
T7879 18035-18038 Entity denotes TSS
T7880 18048-18054 Binding denotes target
T7881 18089-18092 Protein denotes CD8
T7882 18140-18145 Protein denotes Runx3
T7883 18206-18215 Positive_regulation denotes induction
T7884 18219-18224 Protein denotes Eomes
T7885 18242-18251 Gene_expression denotes expressed
T7886 18242-18251 Gene_expression denotes expressed
T7887 18252-18257 Protein denotes Runx3
T7888 18262-18269 Protein denotes Eo-VP16
T7889 18273-18276 Protein denotes CD8
T7890 18291-18296 Protein denotes Runx3
T7891 18339-18342 Protein denotes CD8
T7892 18408-18413 Protein denotes Runx3
T7893 18417-18420 Protein denotes CD8
T7894 18421-18424 Protein denotes CD4
T7895 18433-18436 Protein denotes CD8
T7896 18437-18440 Protein denotes CD4
T7897 18494-18499 Protein denotes Runx3
T7898 18503-18506 Protein denotes CD8
T7899 18607-18612 Protein denotes Runx3
T7900 18616-18619 Protein denotes CD8
T7901 18634-18639 Protein denotes Runx3
T7902 18640-18648 Positive_regulation denotes restored
T7903 18640-18648 Positive_regulation denotes restored
T7904 18640-18648 Positive_regulation denotes restored
T7905 18640-18648 Positive_regulation denotes restored
T7906 18649-18659 Gene_expression denotes expression
T7907 18649-18659 Gene_expression denotes expression
T7908 18649-18659 Gene_expression denotes expression
T7909 18649-18659 Gene_expression denotes expression
T7910 18663-18668 Protein denotes Eomes
T7911 18680-18688 Protein denotes perforin
T7912 18690-18700 Protein denotes granzyme B
T7913 18706-18711 Protein denotes IFN-γ
T7914 18744-18749 Protein denotes Runx3
T7915 18783-18796 Positive_regulation denotes up-regulation
T7916 18800-18805 Protein denotes Runx1
T7917 18817-18827 Negative_regulation denotes suppressed
T7918 18853-18858 Protein denotes Runx3
T7919 18876-18881 Protein denotes Runx1
T7920 18897-18907 Negative_regulation denotes repression
T7921 18911-18916 Protein denotes Runx3
T7922 18927-18934 Protein denotes Eo-VP16
T7923 18943-18954 Positive_regulation denotes up-regulate true
T7924 18955-18963 Protein denotes perforin
T7925 18964-18974 Gene_expression denotes expression
T7926 18980-18989 Gene_expression denotes expressed
T7927 18993-18998 Protein denotes Runx3
T7928 19024-19032 Positive_regulation denotes restored
T7929 19049-19055 Positive_regulation denotes induce
T7930 19056-19061 Protein denotes IFN-γ
T7931 19062-19072 Gene_expression denotes expression
T7932 19150-19158 Protein denotes perforin
T7933 19159-19169 Gene_expression denotes expression
T7934 19170-19178 Positive_regulation denotes requires true
T7935 19170-19178 Positive_regulation denotes requires true
T7936 19179-19184 Protein denotes Runx3
T7937 19189-19194 Protein denotes Eomes
T7938 20890-20896 Positive_regulation denotes defect true
T7939 20890-20896 Positive_regulation denotes defect true
T7940 20890-20896 Positive_regulation denotes defect true
T7941 20890-20896 Positive_regulation denotes defect true
T7942 20900-20908 Protein denotes perforin
T7943 20913-20923 Protein denotes granzyme B
T7944 20924-20934 Gene_expression denotes expression
T7945 20924-20934 Gene_expression denotes expression
T7946 20936-20941 Protein denotes Runx3
T7947 20945-20948 Protein denotes CD8
T7948 21055-21060 Protein denotes Runx3
T7949 21064-21067 Protein denotes CD8
T7950 21173-21176 Protein denotes CD8
T7951 21214-21219 Protein denotes Runx3
T7952 21322-21332 Positive_regulation denotes activation
T7953 21322-21332 Positive_regulation denotes activation
T7954 21340-21348 Protein denotes perforin
T7955 21349-21359 Protein denotes granzyme B
T7956 21373-21382 Negative_regulation denotes defective
T7957 21373-21382 Negative_regulation denotes defective
T7958 21386-21391 Protein denotes Runx3
T7959 21395-21398 Protein denotes CD8
T7960 21554-21557 Protein denotes Fas
T7961 21558-21568 Protein denotes Fas ligand
T11753 22227-22232 Protein denotes Runx1
T11754 22249-22255 Binding denotes target true
T11755 22249-22255 Binding denotes target true
T11756 22265-22270 Protein denotes Runx3
T11757 22286-22291 Protein denotes Runx3
T11758 22292-22303 Positive_regulation denotes contributed
T11759 22292-22303 Positive_regulation denotes contributed
T11760 22292-22303 Positive_regulation denotes contributed
T11761 22319-22329 Gene_expression denotes expression
T11762 22319-22329 Gene_expression denotes expression
T11763 22319-22329 Gene_expression denotes expression
T11764 22333-22336 Protein denotes TNF
T11765 22338-22342 Protein denotes IL-2
T11766 22348-22353 Protein denotes IFN-γ
T11767 22378-22381 Protein denotes TNF
T11768 22386-22390 Protein denotes IL-2
T11769 22396-22407 Positive_regulation denotes requirement
T11770 22396-22407 Positive_regulation denotes requirement
T11771 22412-22417 Protein denotes Runx3
T11772 22418-22426 Negative_regulation denotes subsides
T11773 22418-22426 Negative_regulation denotes subsides
T11774 22484-22489 Protein denotes Runx1
T11775 22500-22511 Negative_regulation denotes derepressed true
T11776 22515-22520 Protein denotes Runx3
T11777 22542-22547 Protein denotes Runx3
T11778 22564-22572 Positive_regulation denotes required
T11779 22577-22582 Protein denotes IFN-γ
T11780 22583-22593 Gene_expression denotes expression
T11781 22632-22636 Regulation denotes role true
T11782 22644-22653 Positive_regulation denotes induction
T11783 22657-22662 Protein denotes Eomes
T11784 22663-22673 Gene_expression denotes expression
T11785 22754-22759 Protein denotes T-bet
T11786 22764-22769 Protein denotes Eomes
T11787 22775-22787 Positive_regulation denotes up-regulated
T11788 22775-22787 Positive_regulation denotes up-regulated
T11789 22820-22823 Protein denotes CD8
T11790 22956-22961 Protein denotes T-bet
T11791 22965-22971 Positive_regulation denotes needed
T11792 23001-23004 Protein denotes CD8
T11793 23032-23039 Gene_expression denotes produce
T11794 23040-23045 Protein denotes IFN-γ
T11795 23117-23122 Protein denotes Eomes
T11796 23133-23140 Positive_regulation denotes induced
T11797 23150-23170 Regulation denotes functions downstream
T11798 23174-23179 Protein denotes Runx3
T11799 23200-23205 Protein denotes T-bet
T11800 23209-23218 Positive_regulation denotes promoting true
T11801 23209-23218 Positive_regulation denotes promoting true
T11802 23229-23239 Gene_expression denotes expression
T11803 23243-23248 Protein denotes IFN-γ
T11804 23283-23288 Protein denotes T-bet
T11805 23293-23298 Protein denotes Eomes
T11806 23304-23314 Positive_regulation denotes contribute
T11807 23304-23314 Positive_regulation denotes contribute
T11808 23318-23326 Protein denotes perforin
T11809 23327-23337 Gene_expression denotes expression
T11810 23363-23368 Protein denotes Eomes
T11811 23369-23376 Positive_regulation denotes induces
T11812 23369-23376 Positive_regulation denotes induces
T11813 23377-23387 Protein denotes granzyme B
T11814 23406-23411 Protein denotes T-bet
T11815 23555-23560 Protein denotes Eomes
T11816 23565-23570 Protein denotes T-bet
T11817 23613-23619 Positive_regulation denotes induce
T11818 23613-23619 Positive_regulation denotes induce
T11819 23613-23619 Positive_regulation denotes induce
T11820 23613-23619 Positive_regulation denotes induce
T11821 23655-23659 Protein denotes Prf1
T11822 23664-23668 Protein denotes Gzmb
T11823 23689-23694 Protein denotes T-bet
T11824 23699-23704 Protein denotes Eomes
T11825 23722-23732 Regulation denotes regulating
T11826 23722-23732 Regulation denotes regulating
T11827 23733-23743 Protein denotes granzyme B
T11828 23748-23756 Protein denotes perforin
T11829 23757-23767 Gene_expression denotes expression
T11830 23757-23767 Gene_expression denotes expression
T11831 23783-23796 Positive_regulation denotes up-regulation
T11832 23783-23796 Positive_regulation denotes up-regulation
T11833 23800-23805 Protein denotes T-bet
T11834 23810-23815 Protein denotes Eomes
T11835 23841-23849 Positive_regulation denotes preceded
T11836 23841-23849 Positive_regulation denotes preceded
T11837 23850-23863 Positive_regulation denotes up-regulation
T11838 23850-23863 Positive_regulation denotes up-regulation
T11839 23867-23871 Protein denotes Gzmb
T11840 23876-23880 Protein denotes Prf1
T11841 23913-23918 Protein denotes T-bet
T11842 23919-23930 Regulation denotes had no role
T11843 23934-23942 Protein denotes perforin
T11844 23943-23953 Gene_expression denotes expression
T11845 23988-23995 Protein denotes Eo-VP16
T11846 24004-24010 Regulation denotes affect true
T11847 24011-24021 Protein denotes granzyme B
T11848 24022-24032 Gene_expression denotes expression
T11849 24038-24047 Gene_expression denotes expressed
T11850 24051-24056 Protein denotes T-bet
T11851 24063-24068 Protein denotes Runx3
T11877 24743-24748 Protein denotes Runx3
T11878 24781-24784 Protein denotes CD4
T11879 24869-24874 Protein denotes T-bet
T11880 24878-24885 Positive_regulation denotes induced
T11881 24905-24910 Protein denotes IFN-γ
T11882 24924-24931 Positive_regulation denotes induces
T11883 24932-24937 Protein denotes Runx3
T11884 24971-24974 Protein denotes CD8
T11885 24997-25002 Protein denotes Runx3
T11886 25006-25014 Positive_regulation denotes required
T11887 25018-25024 Positive_regulation denotes induce
T11888 25056-25061 Protein denotes Eomes
T15042 29143-29146 Protein denotes CD8
T19171 12189-12199 Gene_expression denotes expression true
T19172 12271-12276 Protein denotes IFN-γ
T19173 12315-12319 Protein denotes Prf1
T19174 12324-12328 Protein denotes GzmB
T19175 12329-12344 Transcription denotes mRNA expression true
T19176 12329-12344 Transcription denotes mRNA expression true
T19177 12354-12359 Protein denotes T-bet
T19178 12360-12369 Negative_regulation denotes deficient
T19179 12370-12373 Protein denotes CD8
T19180 12460-12470 Gene_expression denotes expressing
T19181 12471-12481 Protein denotes Eomes-VP16
T19182 12483-12490 Protein denotes Eo-VP16
T19183 12509-12512 Protein denotes GFP
T19184 12707-12717 Protein denotes Granzyme B
T19185 12722-12727 Protein denotes IFN-γ
T19186 12728-12738 Gene_expression denotes expression true
T20168 15770-15775 Protein denotes Runx3
T20169 15780-15785 Protein denotes Eomes
T20170 15793-15797 Protein denotes Prf1
T20893 19429-19439 Protein denotes Eomes-VP16
T20894 19441-19448 Protein denotes Eo-VP16
T20895 19453-19456 Protein denotes Myc
T20896 19457-19462 Protein denotes Runx3
T20897 19464-19469 Protein denotes Runx3
T20898 19678-19692 Positive_regulation denotes Overexpression
T20899 19696-19706 Protein denotes Eomes-VP16
T20900 19735-19740 Protein denotes Eomes
T20901 19830-19834 Protein denotes VP16
T20902 19863-19873 Gene_expression denotes Expression true
T20903 19863-19873 Gene_expression denotes Expression true
T20904 19877-19887 Protein denotes granzyme B
T20905 19892-19897 Protein denotes IFN-γ
T20929 20593-20603 Gene_expression denotes expression
T20930 20593-20603 Gene_expression denotes expression
T20931 20593-20603 Gene_expression denotes expression
T20932 20605-20610 Protein denotes Eomes
T20933 20615-20626 Regulation denotes participate true
T20934 20646-20651 Protein denotes IFN-γ
T20935 20652-20662 Gene_expression denotes expression
T20936 20672-20677 Protein denotes Runx3
T20937 20682-20687 Protein denotes Eomes
T20938 20697-20702 Protein denotes T-bet
T20939 20721-20729 Positive_regulation denotes activate true
T20940 20721-20729 Positive_regulation denotes activate true
T20941 20721-20729 Positive_regulation denotes activate true true
T20942 20730-20738 Protein denotes perforin
T20943 20739-20749 Gene_expression denotes expression
T20944 20789-20795 Regulation denotes effect
T20945 20789-20795 Regulation denotes effect
T20946 20799-20804 Protein denotes T-bet
T20947 20805-20815 Negative_regulation denotes deficiency
T20948 20819-20823 Protein denotes Gzmb
T20949 20837-20847 Protein denotes granzyme B
T20950 20856-20866 Gene_expression denotes expression
T20951 20856-20866 Gene_expression denotes expression true
T16121 29319-29322 Protein denotes CD8
T16122 29477-29486 Protein denotes Annexin V
T16123 29501-29504 Protein denotes CD8
T16124 29608-29611 Protein denotes CD8
T16125 29663-29672 Protein denotes Annexin V
T50 0-5 Protein denotes Runx3
T51 124-127 Protein denotes CD8
T62 750-755 Protein denotes Runx3
T63 780-785 Protein denotes Runx3
T64 786-795 Regulation denotes regulates
T65 786-795 Regulation denotes regulates
T66 786-795 Regulation denotes regulates
T67 786-795 Regulation denotes regulates
T68 796-801 Protein denotes Eomes
T69 802-812 Gene_expression denotes expression
T70 824-834 Gene_expression denotes expression
T71 824-834 Gene_expression denotes expression
T72 824-834 Gene_expression denotes expression
T73 890-895 Protein denotes IFN-γ
T74 897-905 Protein denotes perforin
T75 911-921 Protein denotes granzyme B
T76 1004-1009 Protein denotes Runx3
T52 338-346 Protein denotes perforin
T53 498-507 Positive_regulation denotes induction
T54 511-516 Protein denotes T-bet
T55 526-535 Positive_regulation denotes induction
T56 539-551 Protein denotes Eomesodermin
T57 553-558 Protein denotes Eomes
T58 594-602 Regulation denotes regulate
T59 594-602 Regulation denotes regulate
T60 632-650 Protein denotes interferon (IFN) γ
T61 651-661 Gene_expression denotes expression
T18327 6581-6584 Protein denotes CD8
T18328 6626-6630 Protein denotes Prf1
T18329 6632-6636 Protein denotes Gzmb
T18330 6638-6643 Protein denotes Tbx21
T18331 6645-6650 Protein denotes T-bet
T18332 6657-6662 Protein denotes Eomes
T18333 6663-6678 Transcription denotes mRNA expression true
T18334 6663-6678 Transcription denotes mRNA expression true
T18335 6663-6678 Transcription denotes mRNA expression true
T18336 6663-6678 Transcription denotes mRNA expression true
T18337 6702-6705 Protein denotes CD8
T18338 6965-6968 Protein denotes CD8
T18339 7126-7134 Gene_expression denotes staining true
T18340 7126-7134 Gene_expression denotes staining true
T18341 7126-7134 Gene_expression denotes staining true
T18342 7139-7149 Protein denotes granzyme B
T18343 7151-7156 Protein denotes IFN-γ
T18344 7162-7165 Protein denotes TNF
T18345 7167-7177 Protein denotes Granzyme B
T18346 7178-7186 Gene_expression denotes staining true
T18347 7361-7364 Protein denotes CD8
T18348 7485-7494 Protein denotes Annexin V
T18349 7528-7531 Protein denotes CD8
T18350 7730-7738 Protein denotes perforin
T18351 7739-7749 Protein denotes granzyme B
T13803 25428-25432 Protein denotes IL-2
T13804 25439-25444 Protein denotes IFN-γ
T13805 25451-25454 Protein denotes TNF
T13806 25461-25471 Protein denotes granzyme B
T13807 25478-25481 Protein denotes CD8
T13808 25488-25492 Protein denotes CD25
T13809 25503-25507 Protein denotes CD44
T13810 25514-25518 Protein denotes CD69
T13811 25573-25578 Protein denotes Eomes
T13812 25625-25630 Protein denotes Runx3
T13813 25738-25746 Protein denotes perforin
T13814 25761-25766 Protein denotes Eomes
T13815 25830-25835 Protein denotes T-bet
T13816 25999-26008 Protein denotes Annexin V
T13817 26044-26047 Protein denotes CD8
T13818 26085-26088 Protein denotes CD8
T20134 15071-15076 Protein denotes Runx3
T20135 15134-15139 Protein denotes Runx3
T20136 15141-15146 Protein denotes Eomes
T20137 15148-15153 Protein denotes T-bet
T20138 15159-15167 Protein denotes perforin
T20139 15168-15178 Gene_expression denotes expression true
T20140 15168-15178 Gene_expression denotes expression true
T20141 15168-15178 Gene_expression denotes expression true
T20142 15168-15178 Gene_expression denotes expression true
T20143 15182-15187 Protein denotes Runx3
T20144 15198-15203 Protein denotes Runx3
T20145 15207-15210 Protein denotes CD8
T20146 15247-15254 Protein denotes β-Actin
T20147 15316-15320 Protein denotes Prf1
T20148 15321-15336 Transcription denotes mRNA expression true
T20149 15340-15345 Protein denotes Runx3
T20150 15356-15361 Protein denotes Runx3
T20151 15365-15368 Protein denotes CD8
T20152 15402-15409 Protein denotes β–Actin
T20153 15445-15455 Gene_expression denotes Expression true
T20154 15445-15455 Gene_expression denotes Expression true
T20155 15445-15455 Gene_expression denotes Expression true
T20156 15445-15455 Gene_expression denotes Expression true
T20157 15459-15469 Protein denotes granzyme B
T20158 15471-15476 Protein denotes IFN-γ
T20159 15478-15481 Protein denotes TNF
T20160 15487-15491 Protein denotes IL-2
T20161 15525-15530 Protein denotes Runx3
T20162 15541-15546 Protein denotes Runx3
T20163 15550-15553 Protein denotes CD8
T20164 15627-15637 Protein denotes granzyme B
T20165 15649-15652 Protein denotes GFP
T20166 15748-15755 Binding denotes binding true
T20167 15748-15755 Binding denotes binding true
T20171 16034-16037 Protein denotes CD8
T20172 16124-16128 Protein denotes Prf1
T20173 16147-16152 Protein denotes Runx3
T20174 16175-16180 Protein denotes Eomes
T14365 26357-26360 Protein denotes CD8
T14366 26386-26390 Protein denotes Tcra
T14367 26442-26447 Protein denotes Tbx21
T14368 26585-26588 Protein denotes CD8
T14369 26603-26608 Protein denotes Runx3
T14370 26926-26929 Protein denotes CD8
T14371 27024-27027 Protein denotes CD3
T14372 27050-27054 Protein denotes CD28
T14373 27406-27412 Protein denotes rhIL-2
T16941 31099-31102 Protein denotes CD8
T16942 32123-32128 Protein denotes Eomes
T16943 32150-32155 Protein denotes Runx3
T20876 19206-19211 Protein denotes Runx3
T20877 19212-19220 Regulation denotes controls
T20878 19212-19220 Regulation denotes controls
T20879 19212-19220 Regulation denotes controls
T20880 19212-19220 Regulation denotes controls
T20881 19221-19226 Protein denotes Eomes
T20882 19228-19236 Protein denotes perforin
T20883 19238-19248 Protein denotes granzyme B
T20884 19254-19259 Protein denotes IFN-γ
T20885 19260-19270 Gene_expression denotes expression
T20886 19260-19270 Gene_expression denotes expression
T20887 19260-19270 Gene_expression denotes expression
T20888 19260-19270 Gene_expression denotes expression
T20889 19289-19294 Protein denotes Runx3
T20890 19301-19306 Protein denotes Runx3
T20891 19310-19313 Protein denotes CD8
T20892 19393-19396 Protein denotes GFP
T20906 20120-20130 Protein denotes granzyme B
T20907 20131-20139 Gene_expression denotes staining true
T20908 20238-20241 Protein denotes GFP
T20909 20381-20386 Protein denotes Runx3
T20910 20406-20411 Protein denotes T-bet
T20911 20415-20422 Positive_regulation denotes induced
T20912 20445-20454 Positive_regulation denotes essential
T20913 20465-20470 Protein denotes IFN-γ
T20914 20471-20481 Gene_expression denotes expression
T20915 20483-20488 Protein denotes Runx3
T20916 20492-20499 Gene_expression denotes present
T20917 20509-20512 Protein denotes CD8
T20918 20526-20535 Negative_regulation denotes represses
T20919 20536-20541 Protein denotes Runx1
T20920 20546-20553 Positive_regulation denotes induces
T20921 20546-20553 Positive_regulation denotes induces
T20922 20546-20553 Positive_regulation denotes induces
T20923 20546-20553 Positive_regulation denotes induces
T20924 20554-20559 Protein denotes Eomes
T20925 20561-20569 Protein denotes perforin
T20926 20571-20581 Protein denotes granzyme B
T20927 20587-20592 Protein denotes IFN-γ
T20928 20593-20603 Gene_expression denotes expression
T3307 5478-5488 Gene_expression denotes expression true
T3308 5478-5488 Gene_expression denotes expression true
T3309 5501-5509 Protein denotes perforin
T3310 5514-5524 Protein denotes granzyme B
T3311 5621-5629 Protein denotes perforin
T3312 5631-5641 Protein denotes granzyme B
T3313 5656-5666 Gene_expression denotes expression
T3314 5656-5666 Gene_expression denotes expression
T3315 5674-5677 Protein denotes CD8
T3316 5739-5749 Gene_expression denotes expression
T3317 5739-5749 Gene_expression denotes expression
T3318 5753-5761 Protein denotes perforin
T3319 5778-5786 Protein denotes perforin
T3320 5826-5836 Gene_expression denotes expression
T3321 5855-5863 Protein denotes perforin
T3322 5865-5869 Protein denotes Prf1
T3323 5871-5886 Transcription denotes mRNA expression
T3324 5895-5903 Positive_regulation denotes increase true
T3325 5951-5959 Negative_regulation denotes decrease
T3326 5989-6001 Positive_regulation denotes reexpression
T3327 6051-6061 Protein denotes granzyme B
T3328 6063-6067 Protein denotes Gzmb
T3329 6078-6097 Transcription denotes low or undetectable true
T3330 6132-6144 Positive_regulation denotes up-regulated
T3331 6176-6185 Positive_regulation denotes increased
T3332 6242-6252 Protein denotes granzyme B
T3333 6288-6301 Positive_regulation denotes remained high
T3334 6373-6382 Gene_expression denotes expressed
T3335 6373-6382 Gene_expression denotes expressed
T3336 6397-6402 Protein denotes IFN-γ
T3337 6407-6410 Protein denotes TNF
T3338 6457-6466 Positive_regulation denotes increased
T3339 6457-6466 Positive_regulation denotes increased
T3340 8254-8261 Gene_expression denotes express
T3341 8294-8302 Protein denotes perforin
T3342 8788-8798 Protein denotes granzyme B
T3343 8799-8809 Gene_expression denotes expression
T3344 8886-8894 Protein denotes perforin
T3345 8895-8905 Gene_expression denotes expression
T3346 8933-8940 Positive_regulation denotes induced
T3347 8988-8992 Protein denotes IL-2
T17560 34389-34392 Protein denotes CD8
T17561 34482-34486 Protein denotes IL-2
T11718 21579-21584 Protein denotes Runx3
T11719 21733-21738 Protein denotes Runx3
T11720 21865-21870 Protein denotes Runx3
T11721 21874-21881 Gene_expression denotes present
T11722 21891-21894 Protein denotes CD8
T11723 21931-21940 Negative_regulation denotes represses
T11724 21941-21946 Protein denotes Runx1
T11725 21951-21970 Positive_regulation denotes has a positive role
T11726 21951-21970 Positive_regulation denotes has a positive role
T11727 21951-21970 Positive_regulation denotes has a positive role
T11728 21951-21970 Positive_regulation denotes has a positive role
T11729 21978-21987 Positive_regulation denotes induction
T11730 21978-21987 Positive_regulation denotes induction
T11731 21978-21987 Positive_regulation denotes induction
T11732 21978-21987 Positive_regulation denotes induction
T11733 21991-21996 Protein denotes Eomes
T11734 21998-22008 Protein denotes granzyme B
T11735 22010-22018 Protein denotes perforin
T11736 22024-22029 Protein denotes IFN-γ
T11737 22031-22036 Protein denotes Runx3
T11738 22037-22042 Binding denotes binds
T11739 22037-22042 Binding denotes binds
T11740 22037-22042 Binding denotes binds
T11741 22037-22042 Binding denotes binds
T11742 22037-22042 Binding denotes binds
T11743 22037-22042 Binding denotes binds
T11744 22046-22055 Entity denotes promoters
T11745 22069-22087 Entity denotes regulatory regions
T11746 22135-22141 Regulation denotes effect true
T11747 22135-22141 Regulation denotes effect true
T11748 22135-22141 Regulation denotes effect true
T11749 22150-22160 Gene_expression denotes expression
T11750 22150-22160 Gene_expression denotes expression
T11751 22150-22160 Gene_expression denotes expression
T11752 22217-22222 Protein denotes Eomes
T11852 24100-24105 Protein denotes Eomes
T11853 24106-24115 Negative_regulation denotes deficient
T11854 24229-24238 Negative_regulation denotes deficient
T11855 24242-24247 Protein denotes Eomes
T11856 24315-24320 Protein denotes Runx3
T11857 24326-24331 Protein denotes Eomes
T11858 24332-24341 Negative_regulation denotes deficient
T11859 24342-24345 Protein denotes CD8
T11860 24380-24385 Protein denotes Eomes
T11861 24402-24407 Protein denotes Runx3
T11862 24411-24417 Positive_regulation denotes induce true
T11863 24411-24417 Positive_regulation denotes induce true
T11864 24418-24426 Protein denotes perforin
T11865 24427-24437 Gene_expression denotes expression
T11866 24529-24534 Protein denotes Runx3
T11867 24555-24562 Positive_regulation denotes induced
T11868 24555-24562 Positive_regulation denotes induced
T11869 24577-24582 Protein denotes T-bet
T11870 24587-24592 Protein denotes Eomes
T11871 24597-24603 Protein denotes IL-2Rβ
T11872 24713-24724 Regulation denotes interaction
T11873 24713-24724 Regulation denotes interaction
T11874 24713-24724 Regulation denotes interaction
T11875 24713-24724 Regulation denotes interaction
T11876 24733-24738 Protein denotes T-bet
T7750 13902-13907 Protein denotes Runx3
T7751 13986-13995 Positive_regulation denotes induction
T7752 13999-14004 Protein denotes Eomes
T7753 14013-14018 Protein denotes Runx3
T7754 14029-14038 Gene_expression denotes expressed
T7755 14053-14056 Protein denotes CD8
T7756 14086-14091 Protein denotes T-bet
T7757 14092-14097 Protein denotes Runx3
T7758 14133-14136 Protein denotes CD4
T7759 14176-14181 Protein denotes Runx3
T7760 14227-14230 Protein denotes CD8
T7761 14245-14250 Protein denotes Runx3
T7762 14307-14312 Protein denotes Runx3
T7763 14360-14363 Protein denotes CD8
T7764 14477-14482 Protein denotes Runx3
T7765 14486-14489 Protein denotes CD8
T7766 14589-14599 Gene_expression denotes expression true
T7767 14589-14599 Gene_expression denotes expression true
T7768 14589-14599 Gene_expression denotes expression true
T7769 14603-14611 Protein denotes perforin
T7770 14613-14623 Protein denotes granzyme B
T7871 17846-17860 Entity denotes −1 kb enhancer
T7872 17906-17910 Binding denotes that
T7873 17927-17935 Entity denotes promoter
T7874 17943-17948 Protein denotes Il2rb
T7875 17970-17976 Binding denotes target
T7876 17980-17985 Protein denotes Eomes
T7877 18009-18013 Binding denotes that
T7878 18030-18034 Protein denotes Ifng
T19149 11884-11894 Regulation denotes Regulation
T19150 11884-11894 Regulation denotes Regulation
T19151 11884-11894 Regulation denotes Regulation
T19152 11884-11894 Regulation denotes Regulation
T19153 11884-11894 Regulation denotes Regulation
T19154 11884-11894 Regulation denotes Regulation
T19155 11898-11906 Protein denotes perforin
T19156 11908-11918 Protein denotes granzyme B
T19157 11924-11929 Protein denotes IFN-γ
T19158 11930-11940 Gene_expression denotes expression
T19159 11930-11940 Gene_expression denotes expression
T19160 11930-11940 Gene_expression denotes expression
T19161 11944-11949 Protein denotes T-bet
T19162 11954-11959 Protein denotes Eomes
T19163 11989-11994 Protein denotes IFN-γ
T19164 11995-12005 Gene_expression denotes expression true
T19165 12013-12018 Protein denotes Tbx21
T19166 12027-12032 Protein denotes T-bet
T19167 12033-12042 Negative_regulation denotes deficient
T19168 12044-12049 Protein denotes Tbx21
T19169 12069-12072 Protein denotes CD8
T19170 12183-12188 Protein denotes IFN-γ
T19187 12728-12738 Gene_expression denotes expression true
T19188 12742-12747 Protein denotes Tbx21
T19189 12755-12760 Protein denotes Tbx21
T19190 12839-12844 Protein denotes IFN-γ
T19191 12845-12855 Gene_expression denotes production true
T19192 12881-12888 Protein denotes Eo-VP16
T19193 12901-12904 Protein denotes GFP
T19194 13024-13027 Protein denotes GFP
T19195 13029-13034 Protein denotes IFN-γ
T6037 10885-10893 Protein denotes Perforin
T6038 10898-10908 Protein denotes granzyme B
T6039 10909-10919 Gene_expression denotes expression
T6040 10909-10919 Gene_expression denotes expression
T6041 10940-10949 Regulation denotes regulated true
T6042 10940-10949 Regulation denotes regulated true
T6043 10953-10958 Protein denotes T-bet
T6044 11038-11048 Gene_expression denotes expression true
T6045 11038-11048 Gene_expression denotes expression true
T6046 11038-11048 Gene_expression denotes expression true
T6047 11052-11057 Protein denotes IFN-γ
T6048 11059-11067 Protein denotes perforin
T6049 11073-11083 Protein denotes granzyme B
T6050 11087-11090 Protein denotes CD8
T6051 11112-11117 Protein denotes Tbx21
T6052 11119-11124 Protein denotes T-bet
T6053 11126-11135 Negative_regulation denotes deficient
T6054 11170-11175 Protein denotes Tbx21
T6055 11179-11182 Protein denotes CD8
T6056 11192-11200 Gene_expression denotes produced true
T6057 11201-11206 Protein denotes IFN-γ
T6058 11278-11283 Protein denotes T-bet
T6059 11284-11294 Negative_regulation denotes deficiency
T6060 11332-11335 Protein denotes CD8
T6061 11468-11473 Protein denotes Eomes
T6062 11494-11501 Positive_regulation denotes induced
T6063 11546-11551 Protein denotes T-bet
T6064 11552-11561 Negative_regulation denotes deficient
T6065 11597-11603 Negative_regulation denotes defect true
T6066 11607-11615 Protein denotes perforin
T6067 11616-11631 Transcription denotes mRNA expression
T6068 11701-11710 Negative_regulation denotes reduction
T6069 11714-11718 Protein denotes GzmB
T6070 11727-11732 Protein denotes T-bet
T6071 11733-11742 Negative_regulation denotes deficient
T6072 11817-11825 Negative_regulation denotes decrease
T6073 11829-11839 Gene_expression denotes expression
T6074 11843-11853 Protein denotes granzyme B
T6075 13153-13158 Protein denotes Eomes
T6076 13180-13183 Protein denotes CD8
T6077 13205-13210 Protein denotes Tbx21
T6078 13284-13287 Protein denotes GFP
T6079 13360-13365 Protein denotes Eomes
T6080 13367-13374 Protein denotes Eo-VP16
T6081 13437-13444 Protein denotes Eo-VP16
T6082 13464-13467 Protein denotes GFP
T6083 13480-13489 Positive_regulation denotes increased
T6084 13490-13498 Protein denotes perforin
T6085 13499-13509 Gene_expression denotes expression
T6086 13525-13530 Protein denotes T-bet
T6087 13531-13540 Negative_regulation denotes deficient
T6088 13541-13544 Protein denotes CD8
T6089 13601-13608 Protein denotes Eo-VP16
T6090 13614-13621 Negative_regulation denotes rescued
T6091 13632-13638 Negative_regulation denotes defect
T6092 13642-13647 Protein denotes IFN-γ
T6093 13648-13658 Gene_expression denotes production
T6094 13671-13676 Protein denotes T-bet
T6095 13677-13686 Negative_regulation denotes deficient
T6096 13687-13690 Protein denotes CD8
T6097 13721-13728 Protein denotes Eo-VP16
T6098 13737-13743 Positive_regulation denotes induce true
T6099 13744-13748 Protein denotes GzmB
T6100 13749-13764 Transcription denotes mRNA expression
T16645 30624-30627 Protein denotes CD8
T16646 30931-30941 Protein denotes Eomes-VP16
T2679 4449-4452 Protein denotes CD8
T2680 4484-4487 Protein denotes CD8
T2681 4559-4562 Protein denotes CD3
T2682 4572-4576 Protein denotes CD28
T2683 4699-4703 Protein denotes IL-2
T2684 4705-4711 Protein denotes rhIL-2
T2685 4806-4809 Protein denotes CD8
T2686 5106-5109 Protein denotes CD3
T2687 5115-5119 Protein denotes CD28
T2688 5257-5260 Protein denotes CD8
T2689 5429-5432 Protein denotes CD8
T18118 35102-35105 Protein denotes CD8
T18119 35120-35125 Protein denotes Runx3
T18120 35180-35185 Protein denotes Runx3
T4693 9147-9152 Protein denotes T-bet
T4694 9157-9162 Protein denotes Eomes
T4695 9163-9173 Gene_expression denotes expression
T4696 9163-9173 Gene_expression denotes expression
T4697 9233-9238 Protein denotes T-bet
T4698 9243-9248 Protein denotes Eomes
T4699 9259-9265 Regulation denotes linked
T4700 9259-9265 Regulation denotes linked
T4701 9259-9265 Regulation denotes linked
T4702 9259-9265 Regulation denotes linked
T4703 9259-9265 Regulation denotes linked
T4704 9259-9265 Regulation denotes linked
T4705 9273-9283 Regulation denotes regulation
T4706 9273-9283 Regulation denotes regulation
T4707 9273-9283 Regulation denotes regulation
T4708 9328-9332 Protein denotes Ifng
T4709 9384-9388 Protein denotes Prf1
T4710 9393-9397 Protein denotes GzmB
T4711 9437-9447 Gene_expression denotes expression true
T4712 9437-9447 Gene_expression denotes expression true
T4713 9519-9524 Protein denotes T-bet
T4714 9551-9561 Gene_expression denotes detectable true
T4715 9571-9574 Protein denotes CD8
T4716 9603-9610 Positive_regulation denotes induced
T4717 9640-9648 Positive_regulation denotes remained
T4718 9649-9658 Gene_expression denotes expressed
T4719 9755-9760 Protein denotes Eomes
T4720 9761-9771 Gene_expression denotes expression
T4721 9776-9795 Positive_regulation denotes low or undetectable true
T4722 9841-9844 Protein denotes CD8
T4723 9900-9906 Regulation denotes effect true
T4724 9914-9924 Gene_expression denotes expression
T4725 9960-9969 Positive_regulation denotes induction
T4726 9973-9978 Protein denotes Eomes
T4727 10052-10057 Protein denotes T-bet
T4728 10058-10073 Transcription denotes mRNA expression
T4729 10096-10106 Gene_expression denotes expression
T4730 10110-10114 Protein denotes GzmB
T4731 10132-10137 Protein denotes Eomes
T4732 10160-10169 Gene_expression denotes expressed
T4733 10188-10200 Gene_expression denotes reexpression
T4734 10204-10212 Protein denotes perforin
T4735 10340-10345 Protein denotes T-bet
T4736 10350-10355 Protein denotes Eomes
T4737 10437-10442 Protein denotes T-bet
T4738 10446-10454 Positive_regulation denotes required
T4739 10465-10470 Protein denotes IFN-γ
T4740 10471-10481 Gene_expression denotes production
T4741 10511-10516 Protein denotes Eomes
T4742 10578-10588 Positive_regulation denotes contribute true
T4743 10602-10609 Regulation denotes control
T4744 10613-10621 Protein denotes perforin
T4745 10622-10632 Gene_expression denotes expression
T4746 10700-10706 Positive_regulation denotes induce
T4747 10707-10712 Protein denotes T-bet
T4748 10728-10735 Positive_regulation denotes induces
T4749 10728-10735 Positive_regulation denotes induces
T4750 10736-10741 Protein denotes IFN-γ
T4751 10760-10770 Protein denotes granzyme B
T4752 10786-10791 Protein denotes Eomes
T4753 10795-10802 Positive_regulation denotes induced
T4754 10844-10848 Protein denotes IL-2
T4755 10853-10862 Positive_regulation denotes activates
T4756 10863-10871 Protein denotes perforin
T4757 10872-10882 Gene_expression denotes expression
T14998 27446-27451 Protein denotes Runx3
T14999 27461-27466 Protein denotes Runx3
T15000 27467-27476 Negative_regulation denotes deficient
T15001 27493-27500 Negative_regulation denotes silence true
T15002 27501-27504 Protein denotes CD4
T15003 27505-27515 Gene_expression denotes expression
T15004 27603-27606 Protein denotes CD8
T15005 27621-27626 Protein denotes Runx3
T15006 27640-27643 Protein denotes CD8
T15007 27644-27647 Protein denotes CD4
T15008 27655-27658 Protein denotes CD8
T15009 27659-27662 Protein denotes CD4
T15010 27698-27701 Protein denotes CD4
T15011 27733-27738 Protein denotes Runx3
T15012 27786-27789 Protein denotes CD8
T15013 27790-27793 Protein denotes CD4
T15014 27852-27855 Protein denotes CD8
T15015 27856-27859 Protein denotes CD4
T15016 27914-27917 Protein denotes CD3
T15017 27924-27928 Protein denotes CD28
T15018 28028-28032 Protein denotes IL-2
T15019 28087-28092 Protein denotes Runx3
T15020 28096-28099 Protein denotes CD8
T15021 28148-28151 Protein denotes CD4
T15022 28152-28162 Gene_expression denotes expression
T15023 28196-28201 Protein denotes Runx3
T15024 28292-28305 Positive_regulation denotes up-regulation
T15025 28292-28305 Positive_regulation denotes up-regulation
T15026 28309-28313 Protein denotes CD25
T15027 28318-28322 Protein denotes CD69
T15028 28368-28379 Positive_regulation denotes up-regulate
T15029 28380-28384 Protein denotes CD25
T15030 28386-28391 Protein denotes Runx3
T15031 28395-28398 Protein denotes CD8
T15032 28421-28425 Protein denotes IL-2
T15033 28610-28618 Negative_regulation denotes silenced
T15034 28619-28622 Protein denotes CD4
T15035 28623-28633 Gene_expression denotes expression
T15036 28932-28937 Protein denotes Runx3
T15037 28941-28944 Protein denotes CD8
T15038 28964-28973 Gene_expression denotes coexpress
T15039 28974-28977 Protein denotes CD4
T15040 29063-29068 Protein denotes Runx3
T15041 29134-29139 Protein denotes Runx3
T1403 1318-1325 Gene_expression denotes produce
T1404 2790-2799 Transcription denotes expressed true
T1405 2929-2938 Gene_expression denotes expressed true
T1406 2966-2976 Gene_expression denotes expression
T1407 2980-2992 Positive_regulation denotes up-regulated
T1408 3100-3110 Regulation denotes regulation
T1409 3100-3110 Regulation denotes regulation
T1410 3250-3259 Negative_regulation denotes deficient
T1411 3515-3522 Positive_regulation denotes induced
T1412 3636-3643 Positive_regulation denotes induced
T1413 3677-3685 Positive_regulation denotes sustains
T1414 1318-1325 Gene_expression denotes produce
T1415 3692-3702 Gene_expression denotes expression
T1416 3713-3721 Positive_regulation denotes required
T1417 3745-3755 Gene_expression denotes expression
T1418 3713-3721 Positive_regulation denotes required
R1211 T1482 T1422 themeOf perforin,expression
R1212 T1483 T1424 themeOf T-bet,deficient
R1213 T1484 T1426 themeOf Runx3,lacking
R1214 T1485 T1428 themeOf IFN-γ,expression
R1215 T1486 T1430 themeOf granzyme B,expression
R1216 T1487 T1431 themeOf Runx3,binds
R1229 T1501 T1425 themeOf Tbx21,deletion
R3712 T4730 T4729 themeOf GzmB,expression
R3713 T4731 T4732 themeOf Eomes,expressed
R3714 T4734 T4733 themeOf perforin,reexpression
R3715 T4737 T4738 causeOf T-bet,required
R3716 T4739 T4740 themeOf IFN-γ,production
R3717 T4740 T4738 themeOf production,required
R3718 T4741 T4742 causeOf Eomes,contribute
R3719 T4743 T4742 themeOf control,contribute
R3720 T4744 T4745 themeOf perforin,expression
R3721 T4745 T4743 themeOf expression,control
R3722 T4747 T4746 themeOf T-bet,induce
R3723 T4747 T4749 causeOf T-bet,induces
R3724 T4747 T4748 causeOf T-bet,induces
R3725 T4750 T4749 themeOf IFN-γ,induces
R3726 T4751 T4748 themeOf granzyme B,induces
R3727 T4752 T4753 themeOf Eomes,induced
R3730 T4757 T4755 themeOf expression,activates
R6026 T7752 T7751 themeOf Eomes,induction
R6037 T7782 T7783 causeOf Runx3,required
R6038 T7782 T7785 causeOf Runx3,required
R6039 T7786 T7785 themeOf production,required
R6041 T7788 T7784 themeOf production,required
R6042 T7789 T7788 themeOf IFN-γ,production
R6043 T7790 T7787 themeOf TNF,production
R6044 T7791 T7786 themeOf IL-2,production
R6045 T7793 T7794 themeOf T-bet,up-regulated
R6046 T7796 T7795 themeOf Runx3,induces
R6047 T7797 T7800 causeOf T-bet,induce
R6048 T7797 T7803 causeOf T-bet,silence
R6049 T7798 T7799 causeOf Runx3,induce
R6050 T7798 T7802 causeOf Runx3,silence
R6051 T7801 T7800 themeOf IFN-γ,induce
R6052 T7801 T7799 themeOf IFN-γ,induce
R6053 T7804 T7803 themeOf IL-4,silence
R6054 T7804 T7802 themeOf IL-4,silence
R6055 T7805 T7806 causeOf Runx3,necessary
R6056 T7807 T7808 themeOf Eomes,induction
R6057 T7808 T7806 themeOf induction,necessary
R6058 T7809 T7810 themeOf Eomes,expression
R6059 T7811 T7812 themeOf perforin,expression
R6060 T7813 T7817 causeOf overexpression,led
R6061 T7813 T7818 causeOf overexpression,led
R6062 T7814 T7813 themeOf Eo-VP16,overexpression
R6063 T7815 T7816 themeOf T-bet,deficient
R6064 T7819 T7817 themeOf increase,led
R6065 T7820 T7818 themeOf increase,led
R6066 T7821 T7824 themeOf perforin,expression
R6067 T7822 T7823 themeOf IFN-γ,expression
R6068 T7823 T7820 themeOf expression,increase
R6069 T7824 T7819 themeOf expression,increase
R6070 T7825 T7826 causeOf Runx3,necessary
R6071 T7825 T7833 causeOf Runx3,regulate
R6072 T7825 T7831 causeOf Runx3,regulate
R6073 T7825 T7835 causeOf Runx3,regulate
R6074 T7827 T7828 themeOf Eomes,induction
R6075 T7828 T7826 themeOf induction,necessary
R6076 T7829 T7832 causeOf Eomes,regulate
R6077 T7829 T7834 causeOf Eomes,regulate
R6078 T7829 T7830 causeOf Eomes,regulate
R6079 T7836 T7835 themeOf transcription,regulate
R6080 T7836 T7830 themeOf transcription,regulate
R6081 T7837 T7831 themeOf transcription,regulate
R6082 T7837 T7834 themeOf transcription,regulate
R6083 T7838 T7833 themeOf transcription,regulate
R6084 T7838 T7832 themeOf transcription,regulate
R6085 T7839 T7838 themeOf perforin,transcription
R6086 T7840 T7837 themeOf IFN-γ,transcription
R6087 T7841 T7836 themeOf granzyme B,transcription
R6088 T7842 T7846 themeOf Eomes,bound
R6089 T7842 T7849 themeOf Eomes,bound
R6090 T7842 T7847 themeOf Eomes,bound
R6091 T7842 T7854 themeOf Eomes,associated
R6092 T7843 T7845 themeOf Runx3,bound
R6093 T7843 T7848 themeOf Runx3,bound
R6094 T7843 T7844 themeOf Runx3,bound
R6095 T7843 T7855 themeOf Runx3,associated
R6096 T7850 T7846 themeOf regulatory regions,bound
R6097 T7850 T7851 partOf regulatory regions,Prf1
R6098 T7850 T7849 themeOf regulatory regions,bound
R6099 T7850 T7852 partOf regulatory regions,Ifng
R6100 T7850 T7847 themeOf regulatory regions,bound
R6101 T7850 T7853 partOf regulatory regions,Gzmb
R6102 T7850 T7845 themeOf regulatory regions,bound
R6103 T7850 T7848 themeOf regulatory regions,bound
R6104 T7850 T7844 themeOf regulatory regions,bound
R6105 T7856 T7858 themeOf Runx3,bound
R6106 T7856 T7857 themeOf Runx3,bound
R6107 T7861 T7858 themeOf transcription start sites,bound
R6108 T7861 T7859 partOf transcription start sites,Prf1
R6109 T7861 T7857 themeOf transcription start sites,bound
R6110 T7861 T7860 partOf transcription start sites,Gzmb
R6111 T7866 T7872 themeOf Eomes,that
R6112 T7866 T7877 themeOf Eomes,that
R6113 T7866 T7868 themeOf Eomes,bound
R6114 T7866 T7867 themeOf Eomes,bound
R6115 T7870 T7868 themeOf TSS,bound
R6116 T7870 T7869 partOf TSS,Prf1
R6117 T7871 T7867 themeOf −1 kb enhancer,bound
R6118 T7871 T7869 partOf −1 kb enhancer,Prf1
R6119 T7873 T7872 themeOf promoter,that
R6120 T7873 T7874 partOf promoter,Il2rb
R6121 T7873 T7875 themeOf promoter,target
R6122 T7876 T7875 themeOf Eomes,target
R6123 T7879 T7877 themeOf TSS,that
R6124 T7879 T7878 partOf TSS,Ifng
R6125 T7879 T7880 themeOf TSS,target
R6126 T7882 T7883 causeOf Runx3,induction
R6127 T7884 T7883 themeOf Eomes,induction
R6128 T7887 T7886 themeOf Runx3,expressed
R6129 T7888 T7885 themeOf Eo-VP16,expressed
R6130 T7901 T7905 causeOf Runx3,restored
R6131 T7901 T7904 causeOf Runx3,restored
R6132 T7901 T7903 causeOf Runx3,restored
R6133 T7901 T7902 causeOf Runx3,restored
R6134 T7906 T7904 themeOf expression,restored
R6135 T7907 T7905 themeOf expression,restored
R6136 T7908 T7903 themeOf expression,restored
R6137 T7909 T7902 themeOf expression,restored
R6138 T7910 T7907 themeOf Eomes,expression
R6139 T7911 T7906 themeOf perforin,expression
R6140 T7912 T7908 themeOf granzyme B,expression
R6141 T7913 T7909 themeOf IFN-γ,expression
R6142 T7916 T7915 themeOf Runx1,up-regulation
R6143 T7916 T7917 themeOf Runx1,suppressed
R6144 T7918 T7917 causeOf Runx3,suppressed
R6145 T7919 T7920 themeOf Runx1,repression
R6146 T7921 T7920 causeOf Runx3,repression
R6147 T7922 T7923 causeOf Eo-VP16,up-regulate
R6148 T7922 T7926 themeOf Eo-VP16,expressed
R6149 T7922 T7928 causeOf Eo-VP16,restored
R6150 T7924 T7925 themeOf perforin,expression
R6151 T7925 T7923 themeOf expression,up-regulate
R6152 T7929 T7928 themeOf induce,restored
R6153 T7930 T7931 themeOf IFN-γ,expression
R6154 T7931 T7929 themeOf expression,induce
R6155 T7932 T7933 themeOf perforin,expression
R6156 T7933 T7934 themeOf expression,requires
R6157 T7933 T7935 themeOf expression,requires
R6158 T7936 T7934 causeOf Runx3,requires
R6159 T7936 T7939 causeOf Runx3,defect
R6160 T7936 T7940 causeOf Runx3,defect
R6161 T7937 T7935 causeOf Eomes,requires
R6162 T7937 T7941 causeOf Eomes,defect
R6163 T7937 T7938 causeOf Eomes,defect
R6164 T7942 T7945 themeOf perforin,expression
R6165 T7943 T7944 themeOf granzyme B,expression
R6166 T7944 T7940 themeOf expression,defect
R6167 T7944 T7938 themeOf expression,defect
R6168 T7945 T7939 themeOf expression,defect
R6169 T7945 T7941 themeOf expression,defect
R6170 T7952 T7956 themeOf activation,defective
R6171 T7953 T7957 themeOf activation,defective
R6172 T7954 T7953 themeOf perforin,activation
R6173 T7955 T7952 themeOf granzyme B,activation
R9162 T11720 T11728 causeOf Runx3,has a positive role
R9163 T11720 T11725 causeOf Runx3,has a positive role
R9164 T11720 T11726 causeOf Runx3,has a positive role
R9166 T11729 T11728 themeOf induction,has a positive role
R9167 T11730 T11726 themeOf induction,has a positive role
R9168 T11731 T11725 themeOf induction,has a positive role
R9170 T11733 T11732 themeOf Eomes,induction
R9171 T11734 T11729 themeOf granzyme B,induction
R9172 T11734 T11751 themeOf granzyme B,expression
R9173 T11735 T11731 themeOf perforin,induction
R9174 T11735 T11749 themeOf perforin,expression
R9175 T11736 T11730 themeOf IFN-γ,induction
R9176 T11736 T11750 themeOf IFN-γ,expression
R9177 T11737 T11738 themeOf Runx3,binds
R9178 T11737 T11740 themeOf Runx3,binds
R9179 T11737 T11741 themeOf Runx3,binds
R9180 T11737 T11743 themeOf Runx3,binds
R9181 T11737 T11742 themeOf Runx3,binds
R9182 T11737 T11739 themeOf Runx3,binds
R9183 T11737 T11747 causeOf Runx3,effect
R9184 T11737 T11746 causeOf Runx3,effect
R9185 T11737 T11748 causeOf Runx3,effect
R9186 T11744 T11738 themeOf promoters,binds
R9187 T11744 T11734 partOf promoters,granzyme B
R9188 T11744 T11740 themeOf promoters,binds
R9189 T11744 T11735 partOf promoters,perforin
R9190 T11744 T11741 themeOf promoters,binds
R9191 T11744 T11736 partOf promoters,IFN-γ
R9192 T11745 T11743 themeOf regulatory regions,binds
R9193 T11745 T11734 partOf regulatory regions,granzyme B
R9194 T11745 T11742 themeOf regulatory regions,binds
R9195 T11745 T11735 partOf regulatory regions,perforin
R9196 T11745 T11739 themeOf regulatory regions,binds
R9197 T11745 T11736 partOf regulatory regions,IFN-γ
R9198 T11749 T11746 themeOf expression,effect
R9199 T11750 T11748 themeOf expression,effect
R9200 T11751 T11747 themeOf expression,effect
R9201 T11752 T11755 themeOf Eomes,target
R9202 T11753 T11754 themeOf Runx1,target
R9203 T11756 T11755 themeOf Runx3,target
R9204 T11756 T11754 themeOf Runx3,target
R9205 T11757 T11758 causeOf Runx3,contributed
R9206 T11757 T11760 causeOf Runx3,contributed
R9207 T11757 T11759 causeOf Runx3,contributed
R9208 T11761 T11759 themeOf expression,contributed
R9209 T11762 T11760 themeOf expression,contributed
R9210 T11763 T11758 themeOf expression,contributed
R9211 T11764 T11763 themeOf TNF,expression
R9212 T11765 T11762 themeOf IL-2,expression
R9213 T11766 T11761 themeOf IFN-γ,expression
R9214 T11767 T11770 themeOf TNF,requirement
R9215 T11768 T11769 themeOf IL-2,requirement
R9216 T11769 T11773 themeOf requirement,subsides
R9217 T11770 T11772 themeOf requirement,subsides
R9218 T11771 T11770 causeOf Runx3,requirement
R9219 T11771 T11769 causeOf Runx3,requirement
R9220 T11774 T11772 causeOf Runx1,subsides
R9221 T11774 T11773 causeOf Runx1,subsides
R9222 T11774 T11775 themeOf Runx1,derepressed
R9223 T11777 T11778 causeOf Runx3,required
R9224 T11777 T11781 causeOf Runx3,role
R9225 T11779 T11780 themeOf IFN-γ,expression
R9226 T11780 T11778 themeOf expression,required
R9227 T11782 T11781 themeOf induction,role
R9228 T11783 T11784 themeOf Eomes,expression
R9229 T11784 T11782 themeOf expression,induction
R9230 T11785 T11788 themeOf T-bet,up-regulated
R9231 T11786 T11787 themeOf Eomes,up-regulated
R9233 T11793 T11791 themeOf produce,needed
R9274 T11841 T11842 causeOf T-bet,had no role
R9275 T11843 T11844 themeOf perforin,expression
R9276 T11844 T11842 themeOf expression,had no role
R9277 T11845 T11846 causeOf Eo-VP16,affect
R9278 T11845 T11849 themeOf Eo-VP16,expressed
R9279 T11847 T11848 themeOf granzyme B,expression
R9280 T11848 T11846 themeOf expression,affect
R9281 T11852 T11853 themeOf Eomes,deficient
R9282 T11855 T11854 themeOf Eomes,deficient
R9283 T11857 T11858 themeOf Eomes,deficient
R9284 T11860 T11863 causeOf Eomes,induce
R9285 T11861 T11862 causeOf Runx3,induce
R9286 T11864 T11865 themeOf perforin,expression
R9287 T11865 T11863 themeOf expression,induce
R9288 T11865 T11862 themeOf expression,induce
R9289 T11869 T11867 themeOf T-bet,induced
R9290 T11870 T11868 themeOf Eomes,induced
R9291 T11876 T11875 themeOf T-bet,interaction
R9292 T11876 T11875 causeOf T-bet,interaction
R9293 T11876 T11873 themeOf T-bet,interaction
R9294 T11876 T11872 causeOf T-bet,interaction
R9295 T11877 T11873 causeOf Runx3,interaction
R9296 T11877 T11872 themeOf Runx3,interaction
R9297 T11877 T11874 themeOf Runx3,interaction
R9298 T11877 T11874 causeOf Runx3,interaction
R9299 T11879 T11880 themeOf T-bet,induced
R9300 T11879 T11882 causeOf T-bet,induces
R9301 T11881 T11880 causeOf IFN-γ,induced
R9302 T11883 T11882 themeOf Runx3,induces
R9303 T11885 T11886 causeOf Runx3,required
R9304 T11887 T11886 themeOf induce,required
R9305 T11888 T11887 themeOf Eomes,induce
R15999 T20930 T20923 themeOf expression,induces
R448 T54 T59 causeOf T-bet,regulate
R449 T54 T53 themeOf T-bet,induction
R450 T56 T58 causeOf Eomesodermin,regulate
R451 T56 T55 themeOf Eomesodermin,induction
R452 T57 T56 equivalentTo Eomes,Eomesodermin
R453 T60 T61 themeOf interferon (IFN) γ,expression
R454 T61 T59 themeOf expression,regulate
R455 T61 T58 themeOf expression,regulate
R456 T63 T64 causeOf Runx3,regulates
R457 T63 T67 causeOf Runx3,regulates
R458 T63 T65 causeOf Runx3,regulates
R459 T63 T66 causeOf Runx3,regulates
R460 T68 T69 themeOf Eomes,expression
R461 T69 T64 themeOf expression,regulates
R462 T70 T67 themeOf expression,regulates
R463 T71 T65 themeOf expression,regulates
R464 T72 T66 themeOf expression,regulates
R465 T73 T70 themeOf IFN-γ,expression
R466 T74 T71 themeOf perforin,expression
R467 T75 T72 themeOf granzyme B,expression
R14227 T18328 T18334 themeOf Prf1,mRNA expression
R14228 T18329 T18336 themeOf Gzmb,mRNA expression
R14229 T18330 T18335 themeOf Tbx21,mRNA expression
R14230 T18331 T18330 equivalentTo T-bet,Tbx21
R14231 T18332 T18333 themeOf Eomes,mRNA expression
R14232 T18342 T18340 themeOf granzyme B,staining
R14233 T18343 T18341 themeOf IFN-γ,staining
R14234 T18344 T18339 themeOf TNF,staining
R14235 T18345 T18346 themeOf Granzyme B,staining
R15540 T20135 T20141 themeOf Runx3,expression
R15541 T20136 T20142 themeOf Eomes,expression
R15542 T20137 T20139 themeOf T-bet,expression
R15543 T20138 T20140 themeOf perforin,expression
R15544 T20147 T20148 themeOf Prf1,mRNA expression
R15545 T20157 T20153 themeOf granzyme B,Expression
R15546 T20158 T20154 themeOf IFN-γ,Expression
R15547 T20159 T20156 themeOf TNF,Expression
R15548 T20160 T20155 themeOf IL-2,Expression
R15549 T20168 T20167 themeOf Runx3,binding
R15550 T20169 T20166 themeOf Eomes,binding
R15551 T20170 T20167 themeOf Prf1,binding
R15552 T20170 T20166 themeOf Prf1,binding
R15965 T20876 T20880 causeOf Runx3,controls
R15971 T20883 T20885 themeOf granzyme B,expression
R15972 T20884 T20888 themeOf IFN-γ,expression
R15974 T20886 T20879 themeOf expression,controls
R15976 T20888 T20880 themeOf expression,controls
R15977 T20894 T20893 equivalentTo Eo-VP16,Eomes-VP16
R15978 T20899 T20898 themeOf Eomes-VP16,Overexpression
R15979 T20904 T20903 themeOf granzyme B,Expression
R15980 T20905 T20902 themeOf IFN-γ,Expression
R15981 T20906 T20907 themeOf granzyme B,staining
R15982 T20910 T20911 themeOf T-bet,induced
R15983 T20910 T20912 causeOf T-bet,essential
R15984 T20913 T20914 themeOf IFN-γ,expression
R15985 T20914 T20912 themeOf expression,essential
R15986 T20915 T20916 themeOf Runx3,present
R15987 T20915 T20918 causeOf Runx3,represses
R15988 T20915 T20923 causeOf Runx3,induces
R15992 T20919 T20918 themeOf Runx1,represses
R15993 T20924 T20930 themeOf Eomes,expression
R15966 T20876 T20879 causeOf Runx3,controls
R15967 T20876 T20878 causeOf Runx3,controls
R15968 T20876 T20877 causeOf Runx3,controls
R15969 T20881 T20886 themeOf Eomes,expression
R15970 T20882 T20887 themeOf perforin,expression
R15973 T20885 T20877 themeOf expression,controls
R15975 T20887 T20878 themeOf expression,controls
R15989 T20915 T20921 causeOf Runx3,induces
R15990 T20915 T20922 causeOf Runx3,induces
R15991 T20915 T20920 causeOf Runx3,induces
R15994 T20925 T20931 themeOf perforin,expression
R15995 T20926 T20928 themeOf granzyme B,expression
R15996 T20927 T20929 themeOf IFN-γ,expression
R15997 T20928 T20922 themeOf expression,induces
R15998 T20929 T20920 themeOf expression,induces
R16000 T20931 T20921 themeOf expression,induces
R16001 T20932 T20933 causeOf Eomes,participate
R16002 T20934 T20935 themeOf IFN-γ,expression
R16003 T20935 T20933 themeOf expression,participate
R16004 T20936 T20940 causeOf Runx3,activate
R16005 T20937 T20939 causeOf Eomes,activate
R16006 T20938 T20941 causeOf T-bet,activate
R16007 T20942 T20943 themeOf perforin,expression
R16008 T20943 T20940 themeOf expression,activate
R16009 T20943 T20939 themeOf expression,activate
R16010 T20943 T20941 themeOf expression,activate
R16011 T20946 T20947 themeOf T-bet,deficiency
R16012 T20947 T20944 causeOf deficiency,effect
R16013 T20947 T20945 causeOf deficiency,effect
R16014 T20948 T20950 themeOf Gzmb,expression
R16015 T20949 T20951 themeOf granzyme B,expression
R16016 T20950 T20944 themeOf expression,effect
R16017 T20951 T20945 themeOf expression,effect
R2539 T3309 T3308 themeOf perforin,expression
R2540 T3310 T3307 themeOf granzyme B,expression
R2541 T3311 T3314 themeOf perforin,expression
R2542 T3312 T3313 themeOf granzyme B,expression
R2543 T3318 T3317 themeOf perforin,expression
R2544 T3319 T3316 themeOf perforin,expression
R2545 T3321 T3320 themeOf perforin,expression
R2546 T3321 T3323 themeOf perforin,mRNA expression
R2547 T3322 T3321 equivalentTo Prf1,perforin
R2548 T3323 T3324 themeOf mRNA expression,increase
R2549 T3323 T3325 themeOf mRNA expression,decrease
R2550 T3323 T3326 themeOf mRNA expression,reexpression
R2551 T3327 T3329 themeOf granzyme B,low or undetectable
R2552 T3327 T3330 themeOf granzyme B,up-regulated
R2553 T3327 T3331 themeOf granzyme B,increased
R2554 T3328 T3327 equivalentTo Gzmb,granzyme B
R2555 T3332 T3333 themeOf granzyme B,remained high
R2556 T3334 T3338 themeOf expressed,increased
R2557 T3335 T3339 themeOf expressed,increased
R2558 T3336 T3334 themeOf IFN-γ,expressed
R2559 T3337 T3335 themeOf TNF,expressed
R2560 T3341 T3340 themeOf perforin,express
R2561 T3342 T3343 themeOf granzyme B,expression
R2562 T3344 T3345 themeOf perforin,expression
R2563 T3345 T3346 themeOf expression,induced
R9159 T11720 T11721 themeOf Runx3,present
R9160 T11720 T11723 causeOf Runx3,represses
R9161 T11720 T11727 causeOf Runx3,has a positive role
R9165 T11724 T11723 themeOf Runx1,represses
R9169 T11732 T11727 themeOf induction,has a positive role
R9248 T11810 T11812 causeOf Eomes,induces
R9249 T11813 T11811 themeOf granzyme B,induces
R9251 T11814 T11811 causeOf T-bet,induces
R9252 T11815 T11820 causeOf Eomes,induce
R9253 T11815 T11817 causeOf Eomes,induce
R9254 T11816 T11818 causeOf T-bet,induce
R9255 T11816 T11819 causeOf T-bet,induce
R9256 T11821 T11820 themeOf Prf1,induce
R9257 T11821 T11818 themeOf Prf1,induce
R9258 T11822 T11817 themeOf Gzmb,induce
R9259 T11822 T11819 themeOf Gzmb,induce
R9260 T11823 T11826 causeOf T-bet,regulating
R9261 T11824 T11825 causeOf Eomes,regulating
R9262 T11827 T11830 themeOf granzyme B,expression
R9263 T11828 T11829 themeOf perforin,expression
R9264 T11829 T11825 themeOf expression,regulating
R9265 T11830 T11826 themeOf expression,regulating
R9266 T11831 T11836 causeOf up-regulation,preceded
R9267 T11832 T11835 causeOf up-regulation,preceded
R9268 T11833 T11831 themeOf T-bet,up-regulation
R9269 T11834 T11832 themeOf Eomes,up-regulation
R9270 T11837 T11836 themeOf up-regulation,preceded
R9271 T11838 T11835 themeOf up-regulation,preceded
R9272 T11839 T11837 themeOf Gzmb,up-regulation
R9273 T11840 T11838 themeOf Prf1,up-regulation
R9232 T11790 T11791 causeOf T-bet,needed
R9234 T11794 T11793 themeOf IFN-γ,produce
R9235 T11795 T11796 themeOf Eomes,induced
R9236 T11795 T11797 themeOf Eomes,functions downstream
R9237 T11795 T11800 causeOf Eomes,promoting
R9238 T11798 T11797 causeOf Runx3,functions downstream
R9239 T11799 T11801 causeOf T-bet,promoting
R9240 T11802 T11800 themeOf expression,promoting
R9241 T11802 T11801 themeOf expression,promoting
R9242 T11803 T11802 themeOf IFN-γ,expression
R9243 T11804 T11807 causeOf T-bet,contribute
R9244 T11805 T11806 causeOf Eomes,contribute
R9245 T11808 T11809 themeOf perforin,expression
R9246 T11809 T11807 themeOf expression,contribute
R9247 T11809 T11806 themeOf expression,contribute
R9250 T11813 T11812 themeOf granzyme B,induces
R6027 T7753 T7754 themeOf Runx3,expressed
R6028 T7769 T7766 themeOf perforin,expression
R6029 T7770 T7768 themeOf granzyme B,expression
R6030 T7771 T7767 themeOf IFN-γ,expression
R6031 T7772 T7773 themeOf perforin,expression
R6032 T7773 T7774 themeOf expression,undetectable
R6033 T7777 T7778 themeOf Eomes,expression
R6034 T7779 T7780 themeOf T-bet,expression
R6035 T7780 T7781 themeOf expression,unimpaired
R6036 T7782 T7784 causeOf Runx3,required
R6040 T7787 T7783 themeOf production,required
R6025 T7750 T7751 causeOf Runx3,induction
R14834 T19155 T19160 themeOf perforin,expression
R14835 T19156 T19159 themeOf granzyme B,expression
R14836 T19157 T19158 themeOf IFN-γ,expression
R14837 T19158 T19150 themeOf expression,Regulation
R14838 T19158 T19149 themeOf expression,Regulation
R14839 T19159 T19154 themeOf expression,Regulation
R14840 T19159 T19153 themeOf expression,Regulation
R14841 T19160 T19151 themeOf expression,Regulation
R14842 T19160 T19152 themeOf expression,Regulation
R14843 T19161 T19150 causeOf T-bet,Regulation
R14844 T19161 T19151 causeOf T-bet,Regulation
R14845 T19161 T19153 causeOf T-bet,Regulation
R14846 T19162 T19154 causeOf Eomes,Regulation
R14847 T19162 T19149 causeOf Eomes,Regulation
R14848 T19162 T19152 causeOf Eomes,Regulation
R14849 T19163 T19164 themeOf IFN-γ,expression
R14850 T19166 T19167 themeOf T-bet,deficient
R14851 T19170 T19171 themeOf IFN-γ,expression
R14852 T19173 T19176 themeOf Prf1,mRNA expression
R14853 T19174 T19175 themeOf GzmB,mRNA expression
R14854 T19177 T19178 themeOf T-bet,deficient
R14855 T19181 T19180 themeOf Eomes-VP16,expressing
R14856 T19182 T19181 equivalentTo Eo-VP16,Eomes-VP16
R14857 T19184 T19186 themeOf Granzyme B,expression
R14858 T19185 T19187 themeOf IFN-γ,expression
R14859 T19190 T19191 themeOf IFN-γ,production
R4767 T6043 T6042 causeOf T-bet,regulated
R4769 T6047 T6046 themeOf IFN-γ,expression
R4770 T6048 T6045 themeOf perforin,expression
R4771 T6049 T6044 themeOf granzyme B,expression
R4772 T6051 T6053 themeOf Tbx21,deficient
R4774 T6057 T6056 themeOf IFN-γ,produced
R4775 T6058 T6059 themeOf T-bet,deficiency
R4776 T6061 T6062 themeOf Eomes,induced
R4777 T6063 T6064 themeOf T-bet,deficient
R4778 T6064 T6065 causeOf deficient,defect
R4779 T6066 T6067 themeOf perforin,mRNA expression
R4780 T6067 T6065 themeOf mRNA expression,defect
R4781 T6069 T6068 themeOf GzmB,reduction
R4783 T6071 T6068 causeOf deficient,reduction
R4763 T6037 T6039 themeOf Perforin,expression
R4764 T6038 T6040 themeOf granzyme B,expression
R4765 T6039 T6041 themeOf expression,regulated
R4766 T6040 T6042 themeOf expression,regulated
R4768 T6043 T6041 causeOf T-bet,regulated
R4773 T6052 T6051 equivalentTo T-bet,Tbx21
R4782 T6070 T6071 themeOf T-bet,deficient
R4784 T6073 T6072 themeOf expression,decrease
R4785 T6074 T6073 themeOf granzyme B,expression
R4786 T6080 T6079 equivalentTo Eo-VP16,Eomes
R4787 T6081 T6083 causeOf Eo-VP16,increased
R4788 T6084 T6085 themeOf perforin,expression
R4789 T6085 T6083 themeOf expression,increased
R4790 T6086 T6087 themeOf T-bet,deficient
R4791 T6089 T6090 causeOf Eo-VP16,rescued
R4792 T6091 T6090 themeOf defect,rescued
R4793 T6092 T6093 themeOf IFN-γ,production
R4794 T6093 T6091 themeOf production,defect
R4795 T6094 T6095 themeOf T-bet,deficient
R4796 T6095 T6091 causeOf deficient,defect
R4797 T6097 T6098 causeOf Eo-VP16,induce
R4798 T6099 T6100 themeOf GzmB,mRNA expression
R4799 T6100 T6098 themeOf mRNA expression,induce
R4800 T6101 T6102 themeOf T-bet,deficient
R4801 T6103 T6104 causeOf T-bet,dependence
R4802 T6105 T6106 themeOf GzmB,mRNA expression
R4803 T6106 T6104 themeOf mRNA expression,dependence
R2074 T2684 T2683 equivalentTo rhIL-2,IL-2
R3684 T4694 T4695 themeOf Eomes,expression
R3685 T4697 T4704 causeOf T-bet,linked
R3686 T4697 T4699 causeOf T-bet,linked
R3687 T4697 T4702 causeOf T-bet,linked
R3688 T4697 T4712 themeOf T-bet,expression
R3689 T4698 T4700 causeOf Eomes,linked
R3690 T4698 T4701 causeOf Eomes,linked
R3691 T4698 T4703 causeOf Eomes,linked
R3692 T4698 T4711 themeOf Eomes,expression
R3693 T4705 T4704 themeOf regulation,linked
R3694 T4705 T4700 themeOf regulation,linked
R3695 T4706 T4702 themeOf regulation,linked
R3696 T4706 T4703 themeOf regulation,linked
R3697 T4707 T4699 themeOf regulation,linked
R3698 T4707 T4701 themeOf regulation,linked
R3699 T4708 T4705 themeOf Ifng,regulation
R3700 T4709 T4707 themeOf Prf1,regulation
R3701 T4710 T4706 themeOf GzmB,regulation
R3702 T4713 T4714 themeOf T-bet,detectable
R3703 T4713 T4716 themeOf T-bet,induced
R3704 T4713 T4718 themeOf T-bet,expressed
R3705 T4718 T4717 themeOf expressed,remained
R3706 T4719 T4720 themeOf Eomes,expression
R3707 T4719 T4724 themeOf Eomes,expression
R3708 T4720 T4721 themeOf expression,low or undetectable
R3709 T4724 T4723 themeOf expression,effect
R3710 T4726 T4725 themeOf Eomes,induction
R3711 T4727 T4728 themeOf T-bet,mRNA expression
R3683 T4693 T4696 themeOf T-bet,expression
R3728 T4752 T4755 causeOf Eomes,activates
R3729 T4756 T4757 themeOf perforin,expression
R11608 T14999 T15000 themeOf Runx3,deficient
R11609 T15000 T15001 causeOf deficient,silence
R11610 T15002 T15003 themeOf CD4,expression
R11611 T15003 T15001 themeOf expression,silence
R11612 T15021 T15022 themeOf CD4,expression
R11613 T15026 T15024 themeOf CD25,up-regulation
R11614 T15027 T15025 themeOf CD69,up-regulation
R11615 T15029 T15028 themeOf CD25,up-regulate
R11616 T15034 T15035 themeOf CD4,expression
R11617 T15035 T15033 themeOf expression,silenced
R11618 T15039 T15038 themeOf CD4,coexpress
R1169 T1406 T1407 themeOf expression,up-regulated
R1170 T1415 T1413 themeOf expression,sustains
R1171 T1417 T1416 themeOf expression,required
R1172 T1419 T1418 themeOf expression,required
R1173 T1422 T1423 themeOf expression,unaffected
R1174 T1424 T1423 causeOf deficient,unaffected
R1175 T1426 T1427 causeOf lacking,decreased
R1176 T1426 T1429 causeOf lacking,decreased
R1177 T1428 T1427 themeOf expression,decreased
R1178 T1430 T1429 themeOf expression,decreased
R1183 T1447 T1436 themeOf eomesodermin,deletion
R1184 T1449 T1440 themeOf T-bet,deficient
R1185 T1450 T1441 themeOf Eomes,deficient
R1186 T1451 T1442 themeOf T-bet,targeting
R1187 T1452 T1443 themeOf Eomes,targeting
R1188 T1457 T1444 themeOf Runx3,expressed
R1189 T1458 T1404 themeOf perforin,expressed
R1190 T1461 T1405 themeOf Runx3,expressed
R1191 T1461 T1406 themeOf Runx3,expression
R1192 T1463 T1408 causeOf Runx3,regulation
R1193 T1463 T1409 causeOf Runx3,regulation
R1194 T1464 T1408 themeOf Il4,regulation
R1195 T1465 T1409 themeOf Ifng,regulation
R1197 T1468 T1410 themeOf Runx3,deficient
R1198 T1472 T1411 themeOf T-bet,induced
R1199 T1473 T1412 themeOf Eomes,induced
R1200 T1473 T1413 causeOf Eomes,sustains
R1201 T1474 T1415 themeOf IFN-γ,expression
R1202 T1475 T1416 causeOf Runx3,required
R1203 T1475 T1418 causeOf Runx3,required
R1204 T1476 T1417 themeOf Eomes,expression
R1205 T1477 T1414 themeOf TNF,produce
R1206 T1478 T1419 themeOf perforin,expression
R1207 T1479 T1420 themeOf Eomes,bind
R1208 T1480 T1421 themeOf Runx3,bind
R1209 T1481 T1420 themeOf Prf1,bind
R1210 T1481 T1421 themeOf Prf1,bind
R1179 T1434 T1433 themeOf expression,regulator
R1180 T1437 T1435 themeOf transcription,promote
R1181 T1439 T1438 themeOf transcription,promote
R1182 T1446 T1501 equivalentTo T-bet,Tbx21
R1196 T1466 T1403 themeOf IFN-γ,produce
R1217 T1487 T1432 themeOf Runx3,binds
R1218 T1491 T1433 causeOf Runx3,regulator
R1219 T1492 T1434 themeOf Gzmb,expression
R1220 T1493 T1435 causeOf T-bet,promote
R1221 T1494 T1438 causeOf Eomes,promote
R1222 T1495 T1437 themeOf Ifng,transcription
R1223 T1496 T1439 themeOf Prf1,transcription
R1224 T1498 T1497 equivalentTo Eomes,Eomesodermin
R1225 T1500 T1431 themeOf promoter regions,binds
R1226 T1500 T1489 partOf promoter regions,Ifng
R1227 T1500 T1432 themeOf promoter regions,binds
R1228 T1500 T1490 partOf promoter regions,Gzmb

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T2000 1144-1147 P10966 denotes CD8
T2001 1144-1147 P01732 denotes CD8
T2002 1287-1295 P14222 denotes perforin
T2003 1357-1362 P01579 denotes IFN-γ
T2004 1367-1370 P01375 denotes TNF
T2005 1626-1629 P01730 denotes CD4
T2006 1770-1775 O95936 denotes Eomes
T2007 1781-1786 Q9UL17 denotes T-bet
T2008 1929-1934 Q9UL17 denotes T-bet
T2009 1998-2001 P10966 denotes CD8
T2010 1998-2001 P01732 denotes CD8
T2011 2078-2083 Q9UL17 denotes T-bet
T2012 2088-2093 O95936 denotes Eomes
T2013 2300-2305 Q9UL17 denotes T-bet
T2014 2310-2315 O95936 denotes Eomes
T2015 2496-2499 P10966 denotes CD8
T2016 2496-2499 P01732 denotes CD8
T2017 2618-2622 Q01196 denotes Runx
T2018 2618-2622 Q13761 denotes Runx
T2019 2618-2622 Q13950 denotes Runx
T2020 2728-2731 P01730 denotes CD4
T2021 2732-2735 P10966 denotes CD8
T2022 2732-2735 P01732 denotes CD8
T2023 2762-2767 Q13761 denotes Runx3
T2024 2772-2780 P14222 denotes perforin
T2025 2839-2842 P10966 denotes CD8
T2026 2839-2842 P01732 denotes CD8
T2027 2887-2890 P01730 denotes CD4
T2028 2916-2921 Q13761 denotes Runx3
T2029 2948-2951 P01730 denotes CD4
T2030 3030-3035 Q13761 denotes Runx3
T2031 3179-3183 Q13761 denotes Runx
T2032 3179-3183 Q01196 denotes Runx
T2033 3179-3183 Q13950 denotes Runx
T2034 3217-3220 P01732 denotes CD8
T2035 3217-3220 P10966 denotes CD8
T2036 3244-3249 Q13761 denotes Runx3
T2037 3260-3263 P10966 denotes CD8
T2038 3260-3263 P01732 denotes CD8
T2039 3343-3348 Q13761 denotes Runx3
T2040 3423-3428 Q13761 denotes Runx3
T2041 3506-3511 Q9UL17 denotes T-bet
T2042 3627-3632 O95936 denotes Eomes
T2043 3686-3691 P01579 denotes IFN-γ
T2044 3704-3709 Q13761 denotes Runx3
T2045 3726-3731 O95936 denotes Eomes
T2046 3736-3744 P14222 denotes perforin
T2047 3766-3771 O95936 denotes Eomes
T2048 3776-3781 Q13761 denotes Runx3
T2049 3819-3827 P14222 denotes perforin
T2050 3856-3861 Q9UL17 denotes T-bet
T2051 3895-3900 Q13761 denotes Runx3
T2052 3930-3935 P01579 denotes IFN-γ
T2053 3940-3950 P10144 denotes granzyme B
T2054 3956-3961 Q13761 denotes Runx3
T2055 4114-4119 Q13761 denotes Runx3
T2056 4182-4187 Q9UL17 denotes T-bet
T2057 4192-4197 O95936 denotes Eomes
T3110 4449-4452 P01732 denotes CD8
T3111 4449-4452 P10966 denotes CD8
T3112 4484-4487 P01732 denotes CD8
T3113 4484-4487 P10966 denotes CD8
T3114 4559-4562 P04234 denotes CD3
T3115 4559-4562 P20963 denotes CD3
T3116 4559-4562 P09693 denotes CD3
T3117 4559-4562 P07766 denotes CD3
T3118 4572-4576 P10747 denotes CD28
T3119 4699-4703 P60568 denotes IL-2
T3120 4806-4809 P01732 denotes CD8
T3121 4806-4809 P10966 denotes CD8
T3122 5106-5109 P20963 denotes CD3
T3123 5106-5109 P04234 denotes CD3
T3124 5106-5109 P09693 denotes CD3
T3125 5106-5109 P07766 denotes CD3
T3126 5115-5119 P10747 denotes CD28
T3127 5257-5260 P10966 denotes CD8
T3128 5257-5260 P01732 denotes CD8
T3129 5429-5432 P01732 denotes CD8
T3130 5429-5432 P10966 denotes CD8
T4259 5501-5509 P14222 denotes perforin
T4260 5514-5524 P10144 denotes granzyme B
T4261 5621-5629 P14222 denotes perforin
T4262 5631-5641 P10144 denotes granzyme B
T4263 5674-5677 P01732 denotes CD8
T4264 5674-5677 P10966 denotes CD8
T4265 5753-5761 P14222 denotes perforin
T4266 5778-5786 P14222 denotes perforin
T4267 5810-5818 Q04864 denotes Relative
T4268 5855-5863 P14222 denotes perforin
T4269 5865-5869 P14222 denotes Prf1
T4270 6051-6061 P10144 denotes granzyme B
T4271 6063-6067 P10144 denotes Gzmb
T4272 6242-6252 P10144 denotes granzyme B
T4273 6397-6402 P01579 denotes IFN-γ
T4274 6407-6410 P01375 denotes TNF
T4275 8294-8302 P14222 denotes perforin
T4276 8788-8798 P10144 denotes granzyme B
T4277 8886-8894 P14222 denotes perforin
T4278 8988-8992 P60568 denotes IL-2
T5462 9147-9152 Q9UL17 denotes T-bet
T5463 9157-9162 O95936 denotes Eomes
T5464 9233-9238 Q9UL17 denotes T-bet
T5465 9243-9248 O95936 denotes Eomes
T5466 9328-9332 P01579 denotes Ifng
T5467 9384-9388 P14222 denotes Prf1
T5468 9393-9397 P10144 denotes GzmB
T5469 9519-9524 Q9UL17 denotes T-bet
T5470 9571-9574 P01732 denotes CD8
T5471 9571-9574 P10966 denotes CD8
T5472 9755-9760 O95936 denotes Eomes
T5473 9841-9844 P01732 denotes CD8
T5474 9841-9844 P10966 denotes CD8
T5475 9973-9978 O95936 denotes Eomes
T5476 10052-10057 Q9UL17 denotes T-bet
T5477 10110-10114 P10144 denotes GzmB
T5478 10132-10137 O95936 denotes Eomes
T5479 10204-10212 P14222 denotes perforin
T5480 10340-10345 Q9UL17 denotes T-bet
T5481 10350-10355 O95936 denotes Eomes
T5482 10437-10442 Q9UL17 denotes T-bet
T5483 10465-10470 P01579 denotes IFN-γ
T5484 10511-10516 O95936 denotes Eomes
T5485 10613-10621 P14222 denotes perforin
T5486 10707-10712 Q9UL17 denotes T-bet
T5487 10736-10741 P01579 denotes IFN-γ
T5488 10760-10770 P10144 denotes granzyme B
T5489 10786-10791 O95936 denotes Eomes
T5490 10844-10848 P60568 denotes IL-2
T5491 10863-10871 P14222 denotes perforin
T6840 10885-10893 P14222 denotes Perforin
T6841 10898-10908 P10144 denotes granzyme B
T6842 10953-10958 Q9UL17 denotes T-bet
T6843 11052-11057 P01579 denotes IFN-γ
T6844 11059-11067 P14222 denotes perforin
T6845 11073-11083 P10144 denotes granzyme B
T6846 11087-11090 P10966 denotes CD8
T6847 11087-11090 P01732 denotes CD8
T6848 11112-11117 Q9UL17 denotes Tbx21
T6849 11119-11124 Q9UL17 denotes T-bet
T6850 11170-11175 Q9UL17 denotes Tbx21
T6851 11179-11182 P10966 denotes CD8
T6852 11179-11182 P01732 denotes CD8
T6853 11201-11206 P01579 denotes IFN-γ
T6854 11278-11283 Q9UL17 denotes T-bet
T6855 11332-11335 P10966 denotes CD8
T6856 11332-11335 P01732 denotes CD8
T6857 11468-11473 O95936 denotes Eomes
T6858 11546-11551 Q9UL17 denotes T-bet
T6859 11607-11615 P14222 denotes perforin
T6860 11714-11718 P10144 denotes GzmB
T6861 11727-11732 Q9UL17 denotes T-bet
T6862 11843-11853 P10144 denotes granzyme B
T6863 13153-13158 O95936 denotes Eomes
T6864 13180-13183 P10966 denotes CD8
T6865 13180-13183 P01732 denotes CD8
T6866 13205-13210 Q9UL17 denotes Tbx21
T6867 13284-13287 Q9U6Y4 denotes GFP
T6868 13360-13365 O95936 denotes Eomes
T6869 13367-13374 O95936 denotes Eo-VP16
T6870 13437-13444 O95936 denotes Eo-VP16
T6871 13464-13467 Q9U6Y4 denotes GFP
T6872 13490-13498 P14222 denotes perforin
T6873 13525-13530 Q9UL17 denotes T-bet
T6874 13541-13544 P10966 denotes CD8
T6875 13541-13544 P01732 denotes CD8
T6876 13601-13608 O95936 denotes Eo-VP16
T6877 13642-13647 P01579 denotes IFN-γ
T6878 13671-13676 Q9UL17 denotes T-bet
T6879 13687-13690 P01732 denotes CD8
T6880 13687-13690 P10966 denotes CD8
T6881 13721-13728 O95936 denotes Eo-VP16
T6882 13744-13748 P10144 denotes GzmB
T6883 13781-13786 Q9UL17 denotes T-bet
T6884 13822-13827 Q9UL17 denotes T-bet
T6885 13842-13846 P10144 denotes GzmB
T6886 13892-13899 O95936 denotes Eo-VP16
T9877 13902-13907 Q13761 denotes Runx3
T9878 13999-14004 O95936 denotes Eomes
T9879 14013-14018 Q13761 denotes Runx3
T9880 14053-14056 P01732 denotes CD8
T9881 14053-14056 P10966 denotes CD8
T9882 14086-14091 Q9UL17 denotes T-bet
T9883 14092-14097 Q13761 denotes Runx3
T9884 14133-14136 P01730 denotes CD4
T9885 14176-14181 Q13761 denotes Runx3
T9886 14227-14230 P01732 denotes CD8
T9887 14227-14230 P10966 denotes CD8
T9888 14245-14250 Q13761 denotes Runx3
T9889 14307-14312 Q13761 denotes Runx3
T9890 14360-14363 P01732 denotes CD8
T9891 14360-14363 P10966 denotes CD8
T9892 14477-14482 Q13761 denotes Runx3
T9893 14486-14489 P01732 denotes CD8
T9894 14486-14489 P10966 denotes CD8
T9895 14603-14611 P14222 denotes perforin
T9896 14613-14623 P10144 denotes granzyme B
T9897 14629-14634 P01579 denotes IFN-γ
T9898 14671-14679 P14222 denotes perforin
T9899 14741-14746 Q13761 denotes Runx3
T9900 14797-14802 Q13761 denotes Runx3
T9901 14837-14842 O95936 denotes Eomes
T9902 14868-14873 Q9UL17 denotes T-bet
T9903 14925-14930 Q13761 denotes Runx3
T9904 14970-14975 P01579 denotes IFN-γ
T9905 14985-14988 P01375 denotes TNF
T9906 14992-14996 P60568 denotes IL-2
T9907 15001-15004 P01732 denotes CD8
T9908 15001-15004 P10966 denotes CD8
T9909 16290-16295 Q9UL17 denotes T-bet
T9910 16330-16335 Q13761 denotes Runx3
T9911 16349-16354 Q9UL17 denotes T-bet
T9912 16359-16364 Q13761 denotes Runx3
T9913 16385-16390 P01579 denotes IFN-γ
T9914 16403-16407 P05112 denotes IL-4
T9915 16492-16497 Q13761 denotes Runx3
T9916 16521-16526 O95936 denotes Eomes
T9917 16569-16574 O95936 denotes Eomes
T9918 16606-16614 P14222 denotes perforin
T9919 16662-16669 O95936 denotes Eo-VP16
T9920 16686-16691 Q9UL17 denotes T-bet
T9921 16737-16745 P14222 denotes perforin
T9922 16750-16755 P01579 denotes IFN-γ
T9923 16913-16917 Q13950 denotes Runx
T9924 16913-16917 Q01196 denotes Runx
T9925 16913-16917 Q13761 denotes Runx
T9926 16982-16987 Q13761 denotes Runx3
T9927 17013-17018 O95936 denotes Eomes
T9928 17051-17056 O95936 denotes Eomes
T9929 17111-17119 P14222 denotes perforin
T9930 17121-17126 P01579 denotes IFN-γ
T9931 17132-17142 P10144 denotes granzyme B
T9932 17236-17241 O95936 denotes Eomes
T9933 17246-17251 Q13761 denotes Runx3
T9934 17284-17288 P14222 denotes Prf1
T9935 17290-17294 P01579 denotes Ifng
T9936 17300-17304 P10144 denotes Gzmb
T9937 17401-17406 Q13761 denotes Runx3
T9938 17420-17424 P14222 denotes Prf1
T9939 17429-17433 P10144 denotes Gzmb
T9940 17478-17482 P60568 denotes IL-2
T9941 17529-17533 P14222 denotes Prf1
T9942 17609-17613 P14222 denotes Prf1
T9943 17633-17637 P01579 denotes Ifng
T9944 17755-17759 P01579 denotes Ifng
T9945 17800-17805 O95936 denotes Eomes
T9946 17829-17833 P14222 denotes Prf1
T9947 17980-17985 O95936 denotes Eomes
T9948 18030-18034 P01579 denotes Ifng
T9949 18089-18092 P01732 denotes CD8
T9950 18089-18092 P10966 denotes CD8
T9951 18140-18145 Q13761 denotes Runx3
T9952 18219-18224 O95936 denotes Eomes
T9953 18252-18257 Q13761 denotes Runx3
T9954 18262-18269 O95936 denotes Eo-VP16
T9955 18273-18276 P10966 denotes CD8
T9956 18273-18276 P01732 denotes CD8
T9957 18291-18296 Q13761 denotes Runx3
T9958 18339-18342 P10966 denotes CD8
T9959 18339-18342 P01732 denotes CD8
T9960 18408-18413 Q13761 denotes Runx3
T9961 18417-18420 P01732 denotes CD8
T9962 18417-18420 P10966 denotes CD8
T9963 18421-18424 P01730 denotes CD4
T9964 18433-18436 P01732 denotes CD8
T9965 18433-18436 P10966 denotes CD8
T9966 18437-18440 P01730 denotes CD4
T9967 18494-18499 Q13761 denotes Runx3
T9968 18503-18506 P10966 denotes CD8
T9969 18503-18506 P01732 denotes CD8
T9970 18607-18612 Q13761 denotes Runx3
T9971 18616-18619 P10966 denotes CD8
T9972 18616-18619 P01732 denotes CD8
T9973 18634-18639 Q13761 denotes Runx3
T9974 18663-18668 O95936 denotes Eomes
T9975 18680-18688 P14222 denotes perforin
T9976 18690-18700 P10144 denotes granzyme B
T9977 18706-18711 P01579 denotes IFN-γ
T9978 18744-18749 Q13761 denotes Runx3
T9979 18800-18805 Q01196 denotes Runx1
T9980 18853-18858 Q13761 denotes Runx3
T9981 18876-18881 Q01196 denotes Runx1
T9982 18911-18916 Q13761 denotes Runx3
T9983 18927-18934 O95936 denotes Eo-VP16
T9984 18955-18963 P14222 denotes perforin
T9985 18993-18998 Q13761 denotes Runx3
T9986 19056-19061 P01579 denotes IFN-γ
T9987 19150-19158 P14222 denotes perforin
T9988 19179-19184 Q13761 denotes Runx3
T9989 19189-19194 O95936 denotes Eomes
T9990 20900-20908 P14222 denotes perforin
T9991 20913-20923 P10144 denotes granzyme B
T9992 20936-20941 Q13761 denotes Runx3
T9993 20945-20948 P10966 denotes CD8
T9994 20945-20948 P01732 denotes CD8
T9995 21055-21060 Q13761 denotes Runx3
T9996 21064-21067 P10966 denotes CD8
T9997 21064-21067 P01732 denotes CD8
T9998 21173-21176 P01732 denotes CD8
T9999 21173-21176 P10966 denotes CD8
T10000 21214-21219 Q13761 denotes Runx3
T10001 21340-21348 P14222 denotes perforin
T10002 21349-21359 P10144 denotes granzyme B
T10003 21386-21391 Q13761 denotes Runx3
T10004 21395-21398 P10966 denotes CD8
T10005 21395-21398 P01732 denotes CD8
T10006 21558-21568 P48023 denotes Fas ligand
T12608 21579-21584 Q13761 denotes Runx3
T12609 21733-21738 Q13761 denotes Runx3
T12610 21865-21870 Q13761 denotes Runx3
T12611 21891-21894 P10966 denotes CD8
T12612 21891-21894 P01732 denotes CD8
T12613 21941-21946 Q01196 denotes Runx1
T12614 21991-21996 O95936 denotes Eomes
T12615 21998-22008 P10144 denotes granzyme B
T12616 22010-22018 P14222 denotes perforin
T12617 22024-22029 P01579 denotes IFN-γ
T12618 22031-22036 Q13761 denotes Runx3
T12619 22217-22222 O95936 denotes Eomes
T12620 22227-22232 Q01196 denotes Runx1
T12621 22265-22270 Q13761 denotes Runx3
T12622 22286-22291 Q13761 denotes Runx3
T12623 22333-22336 P01375 denotes TNF
T12624 22338-22342 P60568 denotes IL-2
T12625 22348-22353 P01579 denotes IFN-γ
T12626 22378-22381 P01375 denotes TNF
T12627 22386-22390 P60568 denotes IL-2
T12628 22412-22417 Q13761 denotes Runx3
T12629 22484-22489 Q01196 denotes Runx1
T12630 22515-22520 Q13761 denotes Runx3
T12631 22542-22547 Q13761 denotes Runx3
T12632 22577-22582 P01579 denotes IFN-γ
T12633 22657-22662 O95936 denotes Eomes
T12634 22754-22759 Q9UL17 denotes T-bet
T12635 22764-22769 O95936 denotes Eomes
T12636 22820-22823 P10966 denotes CD8
T12637 22820-22823 P01732 denotes CD8
T12638 22956-22961 Q9UL17 denotes T-bet
T12639 23001-23004 P10966 denotes CD8
T12640 23001-23004 P01732 denotes CD8
T12641 23040-23045 P01579 denotes IFN-γ
T12642 23117-23122 O95936 denotes Eomes
T12643 23174-23179 Q13761 denotes Runx3
T12644 23200-23205 Q9UL17 denotes T-bet
T12645 23243-23248 P01579 denotes IFN-γ
T12646 23283-23288 Q9UL17 denotes T-bet
T12647 23293-23298 O95936 denotes Eomes
T12648 23318-23326 P14222 denotes perforin
T12649 23363-23368 O95936 denotes Eomes
T12650 23377-23387 P10144 denotes granzyme B
T12651 23406-23411 Q9UL17 denotes T-bet
T12652 23451-23459 Q04864 denotes relative
T12653 23555-23560 O95936 denotes Eomes
T12654 23565-23570 Q9UL17 denotes T-bet
T12655 23655-23659 P14222 denotes Prf1
T12656 23664-23668 P10144 denotes Gzmb
T12657 23689-23694 Q9UL17 denotes T-bet
T12658 23699-23704 O95936 denotes Eomes
T12659 23733-23743 P10144 denotes granzyme B
T12660 23748-23756 P14222 denotes perforin
T12661 23800-23805 Q9UL17 denotes T-bet
T12662 23810-23815 O95936 denotes Eomes
T12663 23867-23871 P10144 denotes Gzmb
T12664 23876-23880 P14222 denotes Prf1
T12665 23913-23918 Q9UL17 denotes T-bet
T12666 23934-23942 P14222 denotes perforin
T12667 23988-23995 O95936 denotes Eo-VP16
T12668 24011-24021 P10144 denotes granzyme B
T12669 24051-24056 Q9UL17 denotes T-bet
T12670 24063-24068 Q13761 denotes Runx3
T12671 24100-24105 O95936 denotes Eomes
T12672 24242-24247 O95936 denotes Eomes
T12673 24315-24320 Q13761 denotes Runx3
T12674 24326-24331 O95936 denotes Eomes
T12675 24342-24345 P10966 denotes CD8
T12676 24342-24345 P01732 denotes CD8
T12677 24380-24385 O95936 denotes Eomes
T12678 24402-24407 Q13761 denotes Runx3
T12679 24418-24426 P14222 denotes perforin
T12680 24529-24534 Q13761 denotes Runx3
T12681 24577-24582 Q9UL17 denotes T-bet
T12682 24587-24592 O95936 denotes Eomes
T12683 24733-24738 Q9UL17 denotes T-bet
T12684 24743-24748 Q13761 denotes Runx3
T12685 24781-24784 P01730 denotes CD4
T12686 24869-24874 Q9UL17 denotes T-bet
T12687 24905-24910 P01579 denotes IFN-γ
T12688 24932-24937 Q13761 denotes Runx3
T12689 24971-24974 P01732 denotes CD8
T12690 24971-24974 P10966 denotes CD8
T12691 24997-25002 Q13761 denotes Runx3
T12692 25056-25061 O95936 denotes Eomes
T12693 25183-25187 Q01196 denotes Runx
T12694 25183-25187 Q13950 denotes Runx
T12695 25183-25187 Q13761 denotes Runx
T14195 25428-25432 P60568 denotes IL-2
T14196 25439-25444 P01579 denotes IFN-γ
T14197 25451-25454 P01375 denotes TNF
T14198 25461-25471 P10144 denotes granzyme B
T14199 25478-25481 P01732 denotes CD8
T14200 25478-25481 P10966 denotes CD8
T14201 25488-25492 P01589 denotes CD25
T14202 25573-25578 O95936 denotes Eomes
T14203 25625-25630 Q13761 denotes Runx3
T14204 25761-25766 O95936 denotes Eomes
T14205 25830-25835 Q9UL17 denotes T-bet
T14206 25999-26008 P08758 denotes Annexin V
T14207 26044-26047 P10966 denotes CD8
T14208 26044-26047 P01732 denotes CD8
T14209 26085-26088 P10966 denotes CD8
T14210 26085-26088 P01732 denotes CD8
T14806 26343-26346 P01732 denotes CD8
T14807 26343-26346 P10966 denotes CD8
T14808 26357-26360 P10966 denotes CD8
T14809 26357-26360 P01732 denotes CD8
T14810 26442-26447 Q9UL17 denotes Tbx21
T14811 26585-26588 P01732 denotes CD8
T14812 26585-26588 P10966 denotes CD8
T14813 26603-26608 Q13761 denotes Runx3
T14814 26926-26929 P10966 denotes CD8
T14815 26926-26929 P01732 denotes CD8
T14816 27024-27027 P04234 denotes CD3
T14817 27024-27027 P20963 denotes CD3
T14818 27024-27027 P09693 denotes CD3
T14819 27024-27027 P07766 denotes CD3
T14820 27050-27054 P10747 denotes CD28
T15750 28380-28384 P01589 denotes CD25
T15751 28386-28391 Q13761 denotes Runx3
T15752 28395-28398 P01732 denotes CD8
T15753 28395-28398 P10966 denotes CD8
T15754 28421-28425 P60568 denotes IL-2
T15755 28619-28622 P01730 denotes CD4
T15756 28932-28937 Q13761 denotes Runx3
T15757 28941-28944 P01732 denotes CD8
T15758 28941-28944 P10966 denotes CD8
T15759 28974-28977 P01730 denotes CD4
T15760 29063-29068 Q13761 denotes Runx3
T15761 29134-29139 Q13761 denotes Runx3
T15762 29143-29146 P10966 denotes CD8
T15763 29143-29146 P01732 denotes CD8
T17493 31099-31102 P10966 denotes CD8
T17494 31099-31102 P01732 denotes CD8
T17495 31475-31477 P0A7Z4 denotes pH
T17496 31569-31571 P0A7Z4 denotes pH
T17497 31728-31730 P0A7Z4 denotes pH
T17498 32123-32128 O95936 denotes Eomes
T17499 32150-32155 Q13761 denotes Runx3
T17500 32330-32332 P0A7Z4 denotes pH
T17501 32439-32441 P0A7Z4 denotes pH
T17502 33505-33513 Q04864 denotes relative
T18865 6581-6584 P01732 denotes CD8
T18866 6581-6584 P10966 denotes CD8
T18867 6626-6630 P14222 denotes Prf1
T18868 6632-6636 P10144 denotes Gzmb
T18869 6638-6643 Q9UL17 denotes Tbx21
T18870 6645-6650 Q9UL17 denotes T-bet
T18871 6657-6662 O95936 denotes Eomes
T18872 6702-6705 P10966 denotes CD8
T18873 6702-6705 P01732 denotes CD8
T18874 6857-6865 Q04864 denotes relative
T18875 6965-6968 P10966 denotes CD8
T18876 6965-6968 P01732 denotes CD8
T18877 7048-7056 Q04864 denotes Relative
T18878 7139-7149 P10144 denotes granzyme B
T18879 7151-7156 P01579 denotes IFN-γ
T18880 7162-7165 P01375 denotes TNF
T18881 7167-7177 P10144 denotes Granzyme B
T18882 7200-7208 Q04864 denotes relative
T18883 7361-7364 P01732 denotes CD8
T18884 7361-7364 P10966 denotes CD8
T18885 7485-7494 P08758 denotes Annexin V
T18886 7528-7531 P01732 denotes CD8
T18887 7528-7531 P10966 denotes CD8
T18888 7730-7738 P14222 denotes perforin
T19709 11898-11906 P14222 denotes perforin
T19710 11908-11918 P10144 denotes granzyme B
T19711 11924-11929 P01579 denotes IFN-γ
T19712 11944-11949 Q9UL17 denotes T-bet
T19713 11954-11959 O95936 denotes Eomes
T19714 11989-11994 P01579 denotes IFN-γ
T19715 12013-12018 Q9UL17 denotes Tbx21
T19716 12027-12032 Q9UL17 denotes T-bet
T19717 12044-12049 Q9UL17 denotes Tbx21
T19718 12069-12072 P01732 denotes CD8
T19719 12069-12072 P10966 denotes CD8
T19720 12183-12188 P01579 denotes IFN-γ
T19721 12271-12276 P01579 denotes IFN-γ
T19722 12315-12319 P14222 denotes Prf1
T19723 12324-12328 P10144 denotes GzmB
T19724 12354-12359 Q9UL17 denotes T-bet
T19725 12370-12373 P10966 denotes CD8
T19726 12370-12373 P01732 denotes CD8
T19727 12471-12476 O95936 denotes Eomes
T19728 12483-12490 O95936 denotes Eo-VP16
T19729 12509-12512 Q9U6Y4 denotes GFP
T19730 12523-12526 Q9U6Y4 denotes GFP
T19731 12676-12679 Q9U6Y4 denotes GFP
T19732 12707-12717 P10144 denotes Granzyme B
T19733 12722-12727 P01579 denotes IFN-γ
T19734 12742-12747 Q9UL17 denotes Tbx21
T19735 12755-12760 Q9UL17 denotes Tbx21
T19736 12839-12844 P01579 denotes IFN-γ
T19737 12881-12888 O95936 denotes Eo-VP16
T19738 12901-12904 Q9U6Y4 denotes GFP
T19739 13024-13027 Q9U6Y4 denotes GFP
T19740 13029-13034 P01579 denotes IFN-γ
T20425 15071-15076 Q13761 denotes Runx3
T20426 15134-15139 Q13761 denotes Runx3
T20427 15141-15146 O95936 denotes Eomes
T20428 15148-15153 Q9UL17 denotes T-bet
T20429 15159-15167 P14222 denotes perforin
T20430 15182-15187 Q13761 denotes Runx3
T20431 15198-15203 Q13761 denotes Runx3
T20432 15207-15210 P01732 denotes CD8
T20433 15207-15210 P10966 denotes CD8
T20434 15247-15254 P60709 denotes β-Actin
T20435 15316-15320 P14222 denotes Prf1
T20436 15340-15345 Q13761 denotes Runx3
T20437 15356-15361 Q13761 denotes Runx3
T20438 15365-15368 P10966 denotes CD8
T20439 15365-15368 P01732 denotes CD8
T20440 15402-15409 P60709 denotes β–Actin
T20441 15459-15469 P10144 denotes granzyme B
T20442 15471-15476 P01579 denotes IFN-γ
T20443 15478-15481 P01375 denotes TNF
T20444 15487-15491 P60568 denotes IL-2
T20445 15525-15530 Q13761 denotes Runx3
T20446 15541-15546 Q13761 denotes Runx3
T20447 15550-15553 P01732 denotes CD8
T20448 15550-15553 P10966 denotes CD8
T20449 15627-15637 P10144 denotes granzyme B
T20450 15649-15652 Q9U6Y4 denotes GFP
T20451 15770-15775 Q13761 denotes Runx3
T20452 15780-15785 O95936 denotes Eomes
T20453 15793-15797 P14222 denotes Prf1
T20454 16034-16037 P10966 denotes CD8
T20455 16034-16037 P01732 denotes CD8
T20456 16124-16128 P14222 denotes Prf1
T20457 16147-16152 Q13761 denotes Runx3
T20458 16175-16180 O95936 denotes Eomes
T21567 19206-19211 Q13761 denotes Runx3
T21568 19221-19226 O95936 denotes Eomes
T21569 19228-19236 P14222 denotes perforin
T21570 19238-19248 P10144 denotes granzyme B
T21571 19254-19259 P01579 denotes IFN-γ
T21572 19289-19294 Q13761 denotes Runx3
T21573 19301-19306 Q13761 denotes Runx3
T21574 19310-19313 P01732 denotes CD8
T21575 19310-19313 P10966 denotes CD8
T21576 19393-19396 Q9U6Y4 denotes GFP
T21577 19407-19410 Q9U6Y4 denotes GFP
T21578 19429-19434 O95936 denotes Eomes
T21579 19441-19448 O95936 denotes Eo-VP16
T21580 19453-19456 P01106 denotes Myc
T21581 19457-19462 Q13761 denotes Runx3
T21582 19464-19469 Q13761 denotes Runx3
T21583 19565-19568 Q9U6Y4 denotes GFP
T21584 19696-19701 O95936 denotes Eomes
T21585 19735-19740 O95936 denotes Eomes
T21586 19877-19887 P10144 denotes granzyme B
T21587 19892-19897 P01579 denotes IFN-γ
T21588 20120-20130 P10144 denotes granzyme B
T21589 20238-20241 Q9U6Y4 denotes GFP
T21590 20381-20386 Q13761 denotes Runx3
T21591 20406-20411 Q9UL17 denotes T-bet
T21592 20465-20470 P01579 denotes IFN-γ
T21593 20483-20488 Q13761 denotes Runx3
T21594 20509-20512 P01732 denotes CD8
T21595 20509-20512 P10966 denotes CD8
T21596 20536-20541 Q01196 denotes Runx1
T21597 20554-20559 O95936 denotes Eomes
T21598 20561-20569 P14222 denotes perforin
T21599 20571-20581 P10144 denotes granzyme B
T21600 20587-20592 P01579 denotes IFN-γ
T21601 20605-20610 O95936 denotes Eomes
T21602 20646-20651 P01579 denotes IFN-γ
T21603 20672-20677 Q13761 denotes Runx3
T21604 20682-20687 O95936 denotes Eomes
T21605 20697-20702 Q9UL17 denotes T-bet
T21606 20730-20738 P14222 denotes perforin
T21607 20799-20804 Q9UL17 denotes T-bet
T21608 20819-20823 P10144 denotes Gzmb
T21609 20837-20847 P10144 denotes granzyme B
T15716 27428-27431 P01732 denotes CD8
T15717 27428-27431 P10966 denotes CD8
T15718 27446-27451 Q13761 denotes Runx3
T15719 27461-27466 Q13761 denotes Runx3
T15720 27501-27504 P01730 denotes CD4
T15721 27603-27606 P10966 denotes CD8
T15722 27603-27606 P01732 denotes CD8
T15723 27621-27626 Q13761 denotes Runx3
T15724 27640-27643 P10966 denotes CD8
T15725 27640-27643 P01732 denotes CD8
T15726 27644-27647 P01730 denotes CD4
T15727 27655-27658 P01732 denotes CD8
T15728 27655-27658 P10966 denotes CD8
T15729 27659-27662 P01730 denotes CD4
T15730 27698-27701 P01730 denotes CD4
T15731 27733-27738 Q13761 denotes Runx3
T15732 27786-27789 P01732 denotes CD8
T15733 27786-27789 P10966 denotes CD8
T15734 27790-27793 P01730 denotes CD4
T15735 27852-27855 P01732 denotes CD8
T15736 27852-27855 P10966 denotes CD8
T15737 27856-27859 P01730 denotes CD4
T15738 27914-27917 P20963 denotes CD3
T15739 27914-27917 P09693 denotes CD3
T15740 27914-27917 P04234 denotes CD3
T15741 27914-27917 P07766 denotes CD3
T15742 27924-27928 P10747 denotes CD28
T15743 28028-28032 P60568 denotes IL-2
T15744 28087-28092 Q13761 denotes Runx3
T15745 28096-28099 P10966 denotes CD8
T15746 28096-28099 P01732 denotes CD8
T15747 28148-28151 P01730 denotes CD4
T15748 28196-28201 Q13761 denotes Runx3
T15749 28309-28313 P01589 denotes CD25
T452 0-5 Q13761 denotes Runx3
T453 124-127 P01732 denotes CD8
T454 124-127 P10966 denotes CD8
T455 338-346 P14222 denotes perforin
T456 511-516 Q9UL17 denotes T-bet
T457 553-558 O95936 denotes Eomes
T458 750-755 Q13761 denotes Runx3
T459 780-785 Q13761 denotes Runx3
T460 796-801 O95936 denotes Eomes
T461 890-895 P01579 denotes IFN-γ
T462 897-905 P14222 denotes perforin
T463 911-921 P10144 denotes granzyme B
T464 1004-1009 Q13761 denotes Runx3
T16320 29319-29322 P10966 denotes CD8
T16321 29319-29322 P01732 denotes CD8
T16322 29477-29486 P08758 denotes Annexin V
T16323 29501-29504 P10966 denotes CD8
T16324 29501-29504 P01732 denotes CD8
T16325 29608-29611 P01732 denotes CD8
T16326 29608-29611 P10966 denotes CD8
T16327 29663-29672 P08758 denotes Annexin V
T16903 30388-30391 P10966 denotes CD8
T16904 30388-30391 P01732 denotes CD8
T16905 30624-30627 P10966 denotes CD8
T16906 30624-30627 P01732 denotes CD8
T16907 30931-30936 O95936 denotes Eomes
T18072 34389-34392 P10966 denotes CD8
T18073 34389-34392 P01732 denotes CD8
T18074 34482-34486 P60568 denotes IL-2
T18075 34518-34520 P0A7Z4 denotes pH
T18245 35102-35105 P10966 denotes CD8
T18246 35102-35105 P01732 denotes CD8
T18247 35120-35125 Q13761 denotes Runx3
T18248 35180-35185 Q13761 denotes Runx3
T18889 7739-7749 P10144 denotes granzyme B

test2

Id Subject Object Predicate Lexical cue Negation Speculation
T761 2088-2093 Protein denotes Eomes
T762 2300-2305 Protein denotes T-bet
T763 2310-2315 Protein denotes Eomes
T764 2496-2499 Protein denotes CD8
T765 2728-2731 Protein denotes CD4
T766 2732-2735 Protein denotes CD8
T767 2762-2767 Protein denotes Runx3
T768 2772-2780 Protein denotes perforin
T769 2790-2799 Gene_expression denotes expressed
T770 2839-2842 Protein denotes CD8 true
T771 2887-2890 Protein denotes CD4
T772 2916-2921 Protein denotes Runx3
T773 2929-2938 Gene_expression denotes expressed
T774 2948-2951 Protein denotes CD4
T775 2966-2976 Gene_expression denotes expression
T776 2980-2992 Positive_regulation denotes up-regulated
T777 3030-3035 Protein denotes Runx3
T778 3100-3110 Regulation denotes regulation
T779 3114-3139 Protein denotes the Il4 and Ifng cytokine
T780 3197-3206 Gene_expression denotes expressed
T781 3217-3220 Protein denotes CD8
T782 3244-3249 Protein denotes Runx3
T783 3250-3259 Negative_regulation denotes deficient
T784 3260-3263 Protein denotes CD8
T785 3343-3348 Protein denotes Runx3
T786 3423-3428 Protein denotes Runx3
T787 3506-3511 Protein denotes T-bet
T788 3515-3522 Positive_regulation denotes induced
T789 3627-3632 Protein denotes Eomes
T790 3636-3643 Positive_regulation denotes induced
T791 3677-3685 Positive_regulation denotes sustains
T792 3686-3691 Protein denotes IFN-γ
T793 3692-3702 Gene_expression denotes expression
T794 3704-3709 Protein denotes Runx3
T795 3713-3721 Positive_regulation denotes required
T796 3726-3731 Protein denotes Eomes
T797 3736-3744 Protein denotes perforin
T798 3745-3755 Gene_expression denotes expression
T799 3766-3771 Protein denotes Eomes
T800 3776-3781 Protein denotes Runx3
T801 3782-3786 Binding denotes bind
T802 3790-3804 Protein denotes the Prf1 locus
T803 3819-3827 Protein denotes perforin
T804 3828-3838 Gene_expression denotes expression
T805 3856-3861 Protein denotes T-bet
T806 3862-3871 Negative_regulation denotes deficient
T807 3887-3894 Negative_regulation denotes lacking
T808 3895-3900 Protein denotes Runx3
T809 3906-3915 Negative_regulation denotes decreased
T810 3916-3926 Gene_expression denotes expression
T811 3930-3935 Protein denotes IFN-γ
T812 3940-3950 Protein denotes granzyme B
T813 3956-3961 Protein denotes Runx3
T814 3967-3972 Binding denotes binds
T815 3977-3993 Entity denotes promoter regions
T816 3997-4020 Protein denotes the Ifng and Gzmb genes
T817 4114-4119 Protein denotes Runx3
T818 4133-4142 Regulation denotes regulator
T819 4143-4161 Protein denotes of Gzmb expression
T820 4182-4187 Protein denotes T-bet
T821 4192-4197 Protein denotes Eomes
T822 4216-4223 Positive_regulation denotes promote
T823 4224-4237 Transcription denotes transcription
T824 4241-4264 Protein denotes the Ifng and Prf1 genes
T2668 4449-4452 Protein denotes CD8
T2669 4484-4487 Protein denotes CD8
T2670 4559-4562 Protein denotes CD3
T2671 4572-4576 Protein denotes CD28
T2672 4699-4703 Protein denotes IL-2
T2673 4705-4711 Protein denotes rhIL-2
T2674 4806-4809 Protein denotes CD8
T2675 5106-5109 Protein denotes CD3
T2676 5115-5119 Protein denotes CD28
T2677 5257-5260 Protein denotes CD8
T2678 5429-5432 Protein denotes CD8
T3270 5478-5488 Gene_expression denotes expression true
T3271 5501-5509 Protein denotes perforin true
T3272 5514-5524 Protein denotes granzyme B
T3273 5621-5629 Protein denotes perforin
T3274 5631-5641 Protein denotes granzyme B
T3275 5656-5666 Gene_expression denotes expression
T3276 5674-5677 Protein denotes CD8
T3277 5739-5749 Gene_expression denotes expression
T3278 5753-5761 Protein denotes perforin true
T3279 5778-5786 Protein denotes perforin
T3280 5826-5836 Gene_expression denotes expression
T3281 5855-5863 Protein denotes perforin true
T3282 5865-5869 Protein denotes Prf1
T3283 5871-5886 Transcription denotes mRNA expression
T3284 5895-5903 Positive_regulation denotes increase
T3285 5951-5959 Negative_regulation denotes decrease
T3286 6051-6061 Protein denotes granzyme B
T3287 6063-6067 Protein denotes Gzmb
T3288 6132-6144 Positive_regulation denotes up-regulated
T3289 6176-6185 Positive_regulation denotes increased
T3290 6242-6252 Protein denotes granzyme B
T3291 6265-6274 Gene_expression denotes expressed
T3292 6297-6301 Positive_regulation denotes high
T3293 6373-6382 Gene_expression denotes expressed
T3294 6397-6402 Protein denotes IFN-γ
T3295 6407-6410 Protein denotes TNF
T3296 6411-6422 Positive_regulation denotes in response
T3297 6457-6466 Positive_regulation denotes increased
T3298 8254-8261 Gene_expression denotes express
T3299 8294-8302 Protein denotes perforin
T3300 8307-8311 Negative_regulation denotes lack
T3301 8788-8798 Protein denotes granzyme B
T3302 8799-8809 Gene_expression denotes expression
T3303 8886-8894 Protein denotes perforin
T3304 8895-8905 Gene_expression denotes expression
T3305 8933-8940 Positive_regulation denotes induced
T3306 8988-8992 Protein denotes IL-2
T4644 9147-9152 Protein denotes T-bet
T4645 9157-9162 Protein denotes Eomes
T4646 9163-9173 Gene_expression denotes expression
T4647 9233-9238 Protein denotes T-bet
T4648 9243-9248 Protein denotes Eomes
T4649 9328-9332 Protein denotes Ifng
T4650 9384-9388 Protein denotes Prf1
T4651 9393-9397 Protein denotes GzmB
T4652 9519-9524 Protein denotes T-bet
T4653 9571-9574 Protein denotes CD8
T4654 9603-9610 Positive_regulation denotes induced
T4655 9649-9658 Gene_expression denotes expressed true
T4656 9755-9760 Protein denotes Eomes true
T4657 9761-9771 Gene_expression denotes expression true
T4658 9841-9844 Protein denotes CD8
T4659 9914-9924 Gene_expression denotes expression
T4660 9960-9969 Positive_regulation denotes induction
T4661 9973-9978 Protein denotes Eomes
T4662 10052-10057 Protein denotes T-bet true
T4663 10058-10073 Transcription denotes mRNA expression true
T4664 10083-10091 Positive_regulation denotes preceded
T4665 10096-10106 Gene_expression denotes expression
T4666 10110-10114 Protein denotes GzmB
T4667 10132-10137 Protein denotes Eomes
T4668 10160-10169 Gene_expression denotes expressed
T4669 10188-10200 Gene_expression denotes reexpression
T4670 10204-10212 Protein denotes perforin
T4671 10340-10345 Protein denotes T-bet
T4672 10350-10355 Protein denotes Eomes true
T4673 10437-10442 Protein denotes T-bet
T4674 10446-10454 Positive_regulation denotes required
T4675 10465-10470 Protein denotes IFN-γ
T4676 10471-10481 Gene_expression denotes production
T4677 10511-10516 Protein denotes Eomes
T4678 10578-10588 Positive_regulation denotes contribute
T4679 10602-10609 Regulation denotes control
T4680 10613-10621 Protein denotes perforin
T4681 10622-10632 Gene_expression denotes expression
T4682 10700-10706 Positive_regulation denotes induce
T4683 10707-10712 Protein denotes T-bet
T4684 10728-10735 Positive_regulation denotes induces
T4685 10736-10741 Protein denotes IFN-γ
T4686 10760-10770 Protein denotes granzyme B
T4687 10786-10791 Protein denotes Eomes
T4688 10795-10802 Positive_regulation denotes induced
T4689 10844-10848 Protein denotes IL-2
T4690 10853-10862 Positive_regulation denotes activates
T4691 10863-10871 Protein denotes perforin
T4692 10872-10882 Gene_expression denotes expression
T5971 10885-10893 Protein denotes Perforin
T5972 10898-10908 Protein denotes granzyme B
T5973 10909-10919 Gene_expression denotes expression true
T5974 10940-10949 Regulation denotes regulated true
T5975 11038-11048 Gene_expression denotes expression true
T5976 11052-11057 Protein denotes IFN-γ true
T5977 11059-11067 Protein denotes perforin true
T5978 11073-11083 Protein denotes granzyme B
T5979 11087-11090 Protein denotes CD8 true
T5980 11112-11117 Protein denotes Tbx21
T5981 11119-11124 Protein denotes T-bet
T5982 11126-11135 Negative_regulation denotes deficient
T5983 11170-11175 Protein denotes Tbx21 true
T5984 11179-11182 Protein denotes CD8
T5985 11192-11200 Gene_expression denotes produced
T5986 11201-11206 Protein denotes IFN-γ
T5987 11278-11283 Protein denotes T-bet
T5988 11284-11294 Negative_regulation denotes deficiency
T5989 11332-11335 Protein denotes CD8
T5990 11394-11403 Negative_regulation denotes mitigated
T5991 11468-11473 Protein denotes Eomes
T5992 11494-11501 Positive_regulation denotes induced
T5993 11546-11551 Protein denotes T-bet
T5994 11552-11561 Negative_regulation denotes deficient
T5995 11597-11603 Negative_regulation denotes defect
T5996 11607-11615 Protein denotes perforin true
T5997 11616-11631 Transcription denotes mRNA expression
T5998 11701-11710 Negative_regulation denotes reduction
T5999 11714-11718 Protein denotes GzmB
T6000 11727-11732 Protein denotes T-bet
T6001 11733-11742 Negative_regulation denotes deficient
T6002 11817-11825 Negative_regulation denotes decrease
T6003 11829-11839 Gene_expression denotes expression
T6004 11843-11853 Protein denotes granzyme B
T6005 13153-13158 Protein denotes Eomes
T6006 13180-13183 Protein denotes CD8
T6007 13205-13210 Protein denotes Tbx21
T6008 13284-13287 Protein denotes GFP
T6009 13360-13365 Protein denotes Eomes
T6010 13367-13374 Protein denotes Eo-VP16
T6011 13437-13444 Protein denotes Eo-VP16
T6012 13464-13467 Protein denotes GFP
T6013 13480-13489 Positive_regulation denotes increased
T6014 13490-13498 Protein denotes perforin
T6015 13499-13509 Gene_expression denotes expression
T6016 13525-13530 Protein denotes T-bet
T6017 13531-13540 Negative_regulation denotes deficient
T6018 13541-13544 Protein denotes CD8
T6019 13601-13608 Protein denotes Eo-VP16
T6020 13632-13638 Negative_regulation denotes defect
T6021 13642-13647 Protein denotes IFN-γ
T6022 13648-13658 Gene_expression denotes production
T6023 13671-13676 Protein denotes T-bet
T6024 13677-13686 Negative_regulation denotes deficient
T6025 13687-13690 Protein denotes CD8
T6026 13721-13728 Protein denotes Eo-VP16
T6027 13737-13743 Positive_regulation denotes induce
T6028 13744-13748 Protein denotes GzmB
T6029 13749-13764 Transcription denotes mRNA expression
T6030 13781-13786 Protein denotes T-bet
T6031 13787-13796 Negative_regulation denotes deficient
T6032 13822-13827 Protein denotes T-bet
T6033 13828-13838 Positive_regulation denotes dependence
T6034 13842-13846 Protein denotes GzmB
T6035 13847-13862 Transcription denotes mRNA expression
T6036 13892-13899 Protein denotes Eo-VP16
T7585 13902-13907 Protein denotes Runx3
T7586 13986-13995 Positive_regulation denotes induction
T7587 13999-14004 Protein denotes Eomes true
T7588 14013-14018 Protein denotes Runx3 true
T7589 14029-14038 Gene_expression denotes expressed true
T7590 14053-14056 Protein denotes CD8
T7591 14086-14091 Protein denotes T-bet true
T7592 14092-14097 Protein denotes Runx3 true
T7593 14133-14136 Protein denotes CD4
T7594 14176-14181 Protein denotes Runx3 true
T7595 14227-14230 Protein denotes CD8
T7596 14245-14250 Protein denotes Runx3
T7597 14307-14312 Protein denotes Runx3
T7598 14360-14363 Protein denotes CD8
T7599 14477-14482 Protein denotes Runx3
T7600 14486-14489 Protein denotes CD8
T7601 14589-14599 Gene_expression denotes expression
T7602 14603-14611 Protein denotes perforin
T7603 14613-14623 Protein denotes granzyme B
T7604 14629-14634 Protein denotes IFN-γ
T7605 14671-14679 Protein denotes perforin
T7606 14697-14707 Gene_expression denotes expression
T7607 14741-14746 Protein denotes Runx3 true
T7608 14797-14802 Protein denotes Runx3
T7609 14837-14842 Protein denotes Eomes
T7610 14843-14853 Gene_expression denotes expression
T7611 14868-14873 Protein denotes T-bet
T7612 14874-14884 Gene_expression denotes expression
T7613 14889-14899 Negative_regulation denotes unimpaired
T7614 14925-14930 Protein denotes Runx3
T7615 14935-14943 Positive_regulation denotes required
T7616 14956-14966 Gene_expression denotes production
T7617 14970-14975 Protein denotes IFN-γ
T7618 14985-14988 Protein denotes TNF
T7619 14992-14996 Protein denotes IL-2 true
T7620 15001-15004 Protein denotes CD8
T7621 16290-16295 Protein denotes T-bet
T7622 16299-16311 Positive_regulation denotes up-regulated
T7623 16322-16329 Positive_regulation denotes induces
T7624 16330-16335 Protein denotes Runx3
T7625 16349-16354 Protein denotes T-bet
T7626 16359-16364 Protein denotes Runx3
T7627 16378-16384 Positive_regulation denotes induce
T7628 16385-16390 Protein denotes IFN-γ
T7629 16395-16402 Negative_regulation denotes silence
T7630 16403-16407 Protein denotes IL-4 true
T7631 16492-16497 Protein denotes Runx3 true
T7632 16507-16516 Positive_regulation denotes necessary true
T7633 16521-16526 Protein denotes Eomes true
T7634 16527-16536 Positive_regulation denotes induction true
T7635 16569-16574 Protein denotes Eomes true
T7636 16575-16585 Gene_expression denotes expression
T7637 16606-16614 Protein denotes perforin
T7638 16615-16625 Gene_expression denotes expression
T7639 16644-16658 Positive_regulation denotes overexpression
T7640 16662-16669 Protein denotes Eo-VP16
T7641 16686-16691 Protein denotes T-bet
T7642 16692-16701 Negative_regulation denotes deficient
T7643 16720-16728 Positive_regulation denotes increase
T7644 16737-16745 Protein denotes perforin
T7645 16750-16755 Protein denotes IFN-γ
T7646 16756-16766 Gene_expression denotes expression
T7647 16982-16987 Protein denotes Runx3
T7648 16999-17008 Positive_regulation denotes necessary
T7649 17013-17018 Protein denotes Eomes
T7650 17019-17028 Positive_regulation denotes induction
T7651 17051-17056 Protein denotes Eomes
T7652 17060-17068 Regulation denotes regulate
T7653 17069-17082 Transcription denotes transcription
T7654 17111-17119 Protein denotes perforin
T7655 17121-17126 Protein denotes IFN-γ
T7656 17132-17142 Protein denotes granzyme B
T7657 17236-17241 Protein denotes Eomes
T7658 17246-17251 Protein denotes Runx3
T7659 17252-17257 Binding denotes bound
T7660 17258-17276 Entity denotes regulatory regions true
T7661 17284-17288 Protein denotes Prf1
T7662 17290-17294 Protein denotes Ifng
T7663 17300-17304 Protein denotes Gzmb
T7664 17401-17406 Protein denotes Runx3
T7665 17407-17412 Binding denotes bound
T7666 17420-17424 Protein denotes Prf1
T7667 17429-17433 Protein denotes Gzmb true
T7668 17434-17459 Entity denotes transcription start sites true
T7669 17529-17533 Protein denotes Prf1 true
T7670 17609-17613 Protein denotes Prf1
T7671 17633-17637 Protein denotes Ifng true
T7672 17755-17759 Protein denotes Ifng true
T7673 17800-17805 Protein denotes Eomes
T7674 17806-17811 Binding denotes bound true
T7675 17829-17833 Protein denotes Prf1
T7676 17834-17837 Entity denotes TSS
T7677 17846-17860 Entity denotes −1 kb enhancer
T7678 17867-17874 Binding denotes binding
T7679 17927-17935 Entity denotes promoter
T7680 17943-17948 Protein denotes Il2rb
T7681 17970-17976 Binding denotes target
T7682 17980-17985 Protein denotes Eomes
T7683 18030-18034 Protein denotes Ifng
T7684 18035-18038 Entity denotes TSS
T7685 18089-18092 Protein denotes CD8
T7686 18140-18145 Protein denotes Runx3
T7687 18206-18215 Positive_regulation denotes induction
T7688 18219-18224 Protein denotes Eomes
T7689 18242-18251 Gene_expression denotes expressed
T7690 18252-18257 Protein denotes Runx3
T7691 18262-18269 Protein denotes Eo-VP16
T7692 18273-18276 Protein denotes CD8
T7693 18291-18296 Protein denotes Runx3
T7694 18339-18342 Protein denotes CD8
T7695 18408-18413 Protein denotes Runx3
T7696 18417-18420 Protein denotes CD8
T7697 18421-18424 Protein denotes CD4
T7698 18433-18436 Protein denotes CD8
T7699 18437-18440 Protein denotes CD4
T7700 18494-18499 Protein denotes Runx3
T7701 18503-18506 Protein denotes CD8
T7702 18607-18612 Protein denotes Runx3
T7703 18616-18619 Protein denotes CD8
T7704 18634-18639 Protein denotes Runx3
T7705 18649-18659 Gene_expression denotes expression
T7706 18663-18668 Protein denotes Eomes
T7707 18680-18688 Protein denotes perforin
T7708 18690-18700 Protein denotes granzyme B
T7709 18706-18711 Protein denotes IFN-γ
T7710 18744-18749 Protein denotes Runx3
T7711 18783-18796 Positive_regulation denotes up-regulation
T7712 18800-18805 Protein denotes Runx1
T7713 18817-18827 Negative_regulation denotes suppressed
T7714 18853-18858 Protein denotes Runx3
T7715 18876-18881 Protein denotes Runx1
T7716 18897-18907 Negative_regulation denotes repression
T7717 18911-18916 Protein denotes Runx3
T7718 18927-18934 Protein denotes Eo-VP16
T7719 18943-18954 Positive_regulation denotes up-regulate
T7720 18955-18963 Protein denotes perforin
T7721 18964-18974 Gene_expression denotes expression
T7722 18980-18989 Gene_expression denotes expressed
T7723 18993-18998 Protein denotes Runx3
T7724 19049-19055 Positive_regulation denotes induce
T7725 19056-19061 Protein denotes IFN-γ
T7726 19062-19072 Gene_expression denotes expression
T7727 19150-19158 Protein denotes perforin
T7728 19159-19169 Gene_expression denotes expression
T7729 19170-19178 Positive_regulation denotes requires
T7730 19179-19184 Protein denotes Runx3
T7731 19189-19194 Protein denotes Eomes
T7732 20890-20896 Negative_regulation denotes defect
T7733 20900-20908 Protein denotes perforin
T7734 20913-20923 Protein denotes granzyme B
T7735 20924-20934 Gene_expression denotes expression
T7736 20936-20941 Protein denotes Runx3
T7737 20945-20948 Protein denotes CD8
T7738 21055-21060 Protein denotes Runx3
T7739 21064-21067 Protein denotes CD8
T7740 21173-21176 Protein denotes CD8
T7741 21214-21219 Protein denotes Runx3
T7742 21322-21332 Positive_regulation denotes activation
T7743 21340-21348 Protein denotes perforin
T7744 21349-21359 Protein denotes granzyme B
T7745 21373-21382 Negative_regulation denotes defective
T7746 21386-21391 Protein denotes Runx3
T7747 21395-21398 Protein denotes CD8
T7748 21554-21557 Protein denotes Fas
T7749 21558-21568 Protein denotes Fas ligand
T29 0-5 Protein denotes Runx3
T30 124-127 Protein denotes CD8
T31 338-346 Protein denotes perforin
T32 498-507 Positive_regulation denotes induction
T33 511-516 Protein denotes T-bet
T34 526-535 Positive_regulation denotes induction
T35 539-551 Protein denotes Eomesodermin
T36 553-558 Protein denotes Eomes
T37 594-602 Regulation denotes regulate
T38 632-650 Protein denotes interferon (IFN) γ
T39 651-661 Gene_expression denotes expression
T40 750-755 Protein denotes Runx3
T41 780-785 Protein denotes Runx3
T42 786-795 Regulation denotes regulates
T43 796-801 Protein denotes Eomes
T44 802-812 Gene_expression denotes expression
T45 824-834 Gene_expression denotes expression
T46 890-895 Protein denotes IFN-γ
T47 897-905 Protein denotes perforin
T48 911-921 Protein denotes granzyme B
T49 1004-1009 Protein denotes Runx3
T743 1144-1147 Protein denotes CD8
T744 1287-1295 Protein denotes perforin
T745 1318-1325 Gene_expression denotes produce
T746 1357-1362 Protein denotes IFN-γ
T747 1367-1370 Protein denotes TNF
T748 1626-1629 Protein denotes CD4
T749 1756-1768 Protein denotes Eomesodermin
T750 1770-1775 Protein denotes Eomes
T751 1781-1786 Protein denotes T-bet true
T752 1897-1905 Negative_regulation denotes deletion true
T753 1909-1918 Protein denotes the Tbx21 true
T754 1929-1934 Protein denotes T-bet
T755 1936-1958 Protein denotes and eomesodermin genes
T756 1962-1972 Binding denotes associated
T757 1998-2001 Protein denotes CD8
T758 2019-2025 Protein denotes IL-2Rβ
T759 2059-2068 Negative_regulation denotes deficient
T760 2078-2083 Protein denotes T-bet
T11595 21579-21584 Protein denotes Runx3
T11596 21733-21738 Protein denotes Runx3
T11597 21865-21870 Protein denotes Runx3
T11598 21891-21894 Protein denotes CD8
T11599 21931-21940 Negative_regulation denotes represses
T11600 21941-21946 Protein denotes Runx1
T11601 21951-21970 Positive_regulation denotes has a positive role
T11602 21978-21987 Positive_regulation denotes induction
T11603 21991-21996 Protein denotes Eomes
T11604 21998-22008 Protein denotes granzyme B
T11605 22010-22018 Protein denotes perforin
T11606 22024-22029 Protein denotes IFN-γ
T11607 22031-22036 Protein denotes Runx3
T11608 22037-22042 Binding denotes binds
T11609 22046-22055 Entity denotes promoters
T11610 22069-22087 Entity denotes regulatory regions
T11611 22217-22222 Protein denotes Eomes true
T11612 22227-22232 Protein denotes Runx1
T11613 22265-22270 Protein denotes Runx3 true
T11614 22286-22291 Protein denotes Runx3
T11615 22292-22303 Positive_regulation denotes contributed true
T11616 22319-22329 Gene_expression denotes expression
T11617 22333-22336 Protein denotes TNF true
T11618 22338-22342 Protein denotes IL-2 true
T11619 22348-22353 Protein denotes IFN-γ
T11620 22378-22381 Protein denotes TNF
T11621 22386-22390 Protein denotes IL-2
T11622 22412-22417 Protein denotes Runx3
T11623 22484-22489 Protein denotes Runx1
T11624 22515-22520 Protein denotes Runx3
T11625 22542-22547 Protein denotes Runx3
T11626 22564-22572 Positive_regulation denotes required
T11627 22577-22582 Protein denotes IFN-γ
T11628 22583-22593 Gene_expression denotes expression
T11629 22632-22636 Regulation denotes role true
T11630 22644-22653 Positive_regulation denotes induction
T11631 22657-22662 Protein denotes Eomes
T11632 22663-22673 Gene_expression denotes expression true
T11633 22754-22759 Protein denotes T-bet
T11634 22764-22769 Protein denotes Eomes
T11635 22775-22787 Positive_regulation denotes up-regulated
T11636 22820-22823 Protein denotes CD8
T11637 22956-22961 Protein denotes T-bet
T11638 23001-23004 Protein denotes CD8
T11639 23032-23039 Gene_expression denotes produce
T11640 23040-23045 Protein denotes IFN-γ
T11641 23117-23122 Protein denotes Eomes true
T11642 23133-23140 Positive_regulation denotes induced
T11643 23160-23170 Regulation denotes downstream true
T11644 23174-23179 Protein denotes Runx3
T11645 23200-23205 Protein denotes T-bet
T11646 23209-23218 Positive_regulation denotes promoting
T11647 23229-23239 Gene_expression denotes expression
T11648 23243-23248 Protein denotes IFN-γ
T11649 23283-23288 Protein denotes T-bet
T11650 23293-23298 Protein denotes Eomes
T11651 23304-23314 Positive_regulation denotes contribute
T11652 23318-23326 Protein denotes perforin
T11653 23327-23337 Gene_expression denotes expression
T11654 23363-23368 Protein denotes Eomes
T11655 23369-23376 Positive_regulation denotes induces
T11656 23377-23387 Protein denotes granzyme B
T11657 23406-23411 Protein denotes T-bet
T11658 23555-23560 Protein denotes Eomes
T11659 23565-23570 Protein denotes T-bet
T11660 23613-23619 Positive_regulation denotes induce
T11661 23655-23659 Protein denotes Prf1
T11662 23664-23668 Protein denotes Gzmb
T11663 23689-23694 Protein denotes T-bet
T11664 23699-23704 Protein denotes Eomes
T11665 23722-23732 Regulation denotes regulating true
T11666 23733-23743 Protein denotes granzyme B
T11667 23748-23756 Protein denotes perforin
T11668 23757-23767 Gene_expression denotes expression
T11669 23783-23796 Positive_regulation denotes up-regulation
T11670 23800-23805 Protein denotes T-bet
T11671 23810-23815 Protein denotes Eomes true
T11672 23850-23863 Positive_regulation denotes up-regulation
T11673 23867-23871 Protein denotes Gzmb true
T11674 23876-23880 Protein denotes Prf1
T11675 23913-23918 Protein denotes T-bet
T11676 23926-23930 Regulation denotes role
T11677 23934-23942 Protein denotes perforin
T11678 23943-23953 Gene_expression denotes expression
T11679 23988-23995 Protein denotes Eo-VP16
T11680 24004-24010 Regulation denotes affect
T11681 24011-24021 Protein denotes granzyme B
T11682 24022-24032 Gene_expression denotes expression
T11683 24038-24047 Gene_expression denotes expressed
T11684 24051-24056 Protein denotes T-bet
T11685 24063-24068 Protein denotes Runx3
T11686 24100-24105 Protein denotes Eomes
T11687 24106-24115 Negative_regulation denotes deficient
T11688 24229-24238 Negative_regulation denotes deficient
T11689 24242-24247 Protein denotes Eomes
T11690 24315-24320 Protein denotes Runx3
T11691 24326-24331 Protein denotes Eomes
T11692 24332-24341 Negative_regulation denotes deficient
T11693 24342-24345 Protein denotes CD8
T11694 24380-24385 Protein denotes Eomes
T11695 24402-24407 Protein denotes Runx3
T11696 24411-24417 Positive_regulation denotes induce
T11697 24418-24426 Protein denotes perforin
T11698 24427-24437 Gene_expression denotes expression
T11699 24529-24534 Protein denotes Runx3
T11700 24555-24562 Positive_regulation denotes induced
T11701 24577-24582 Protein denotes T-bet
T11702 24587-24592 Protein denotes Eomes
T11703 24597-24603 Protein denotes IL-2Rβ
T11704 24713-24724 Binding denotes interaction
T11705 24733-24738 Protein denotes T-bet
T11706 24743-24748 Protein denotes Runx3
T11707 24781-24784 Protein denotes CD4
T11708 24869-24874 Protein denotes T-bet
T11709 24878-24885 Positive_regulation denotes induced
T11710 24905-24910 Protein denotes IFN-γ
T11711 24924-24931 Positive_regulation denotes induces
T11712 24932-24937 Protein denotes Runx3
T11713 24971-24974 Protein denotes CD8
T11714 24997-25002 Protein denotes Runx3
T11715 25006-25014 Positive_regulation denotes required
T11716 25018-25024 Positive_regulation denotes induce
T11717 25056-25061 Protein denotes Eomes
T13786 25428-25432 Protein denotes IL-2
T13787 25439-25444 Protein denotes IFN-γ
T13788 25451-25454 Protein denotes TNF
T13789 25461-25471 Protein denotes granzyme B
T13790 25478-25481 Protein denotes CD8
T13791 25488-25492 Protein denotes CD25
T13792 25503-25507 Protein denotes CD44
T13793 25514-25518 Protein denotes CD69
T13794 25573-25578 Protein denotes Eomes
T13795 25625-25630 Protein denotes Runx3
T13796 25644-25652 Gene_expression denotes produced
T13797 25734-25746 Protein denotes antiperforin
T13798 25761-25766 Protein denotes Eomes
T13799 25830-25835 Protein denotes T-bet
T13800 25999-26008 Protein denotes Annexin V
T13801 26044-26047 Protein denotes CD8
T13802 26085-26088 Protein denotes CD8
T14356 26357-26360 Protein denotes CD8
T14357 26386-26390 Protein denotes Tcra
T14358 26442-26447 Protein denotes Tbx21
T14359 26585-26588 Protein denotes CD8
T14360 26603-26608 Protein denotes Runx3
T14361 26926-26929 Protein denotes CD8
T14362 27024-27027 Protein denotes CD3
T14363 27050-27054 Protein denotes CD28
T14364 27406-27412 Protein denotes rhIL-2
T14952 27446-27451 Protein denotes Runx3
T14953 27461-27466 Protein denotes Runx3 true
T14954 27467-27476 Negative_regulation denotes deficient
T14955 27493-27500 Negative_regulation denotes silence
T14956 27501-27504 Protein denotes CD4
T14957 27505-27515 Gene_expression denotes expression
T14958 27603-27606 Protein denotes CD8
T14959 27621-27626 Protein denotes Runx3
T14960 27640-27643 Protein denotes CD8
T14961 27644-27647 Protein denotes CD4
T14962 27655-27658 Protein denotes CD8
T14963 27659-27662 Protein denotes CD4
T14964 27698-27701 Protein denotes CD4
T14965 27733-27738 Protein denotes Runx3
T14966 27786-27789 Protein denotes CD8
T14967 27790-27793 Protein denotes CD4
T14968 27852-27855 Protein denotes CD8
T14969 27856-27859 Protein denotes CD4
T14970 27914-27917 Protein denotes CD3
T14971 27924-27928 Protein denotes CD28
T14972 28028-28032 Protein denotes IL-2
T14973 28087-28092 Protein denotes Runx3
T14974 28096-28099 Protein denotes CD8
T14975 28122-28130 Negative_regulation denotes impaired
T14976 28148-28151 Protein denotes CD4
T14977 28152-28162 Gene_expression denotes expression
T14978 28196-28201 Protein denotes Runx3
T14979 28292-28305 Positive_regulation denotes up-regulation
T14980 28309-28313 Protein denotes CD25
T14981 28318-28322 Protein denotes CD69
T14982 28368-28379 Positive_regulation denotes up-regulate
T14983 28380-28384 Protein denotes CD25
T14984 28386-28391 Protein denotes Runx3
T14985 28395-28398 Protein denotes CD8
T14986 28421-28425 Protein denotes IL-2
T14987 28426-28441 Negative_regulation denotes supplementation
T14988 28610-28618 Negative_regulation denotes silenced
T14989 28619-28622 Protein denotes CD4
T14990 28623-28633 Gene_expression denotes expression
T14991 28932-28937 Protein denotes Runx3
T14992 28941-28944 Protein denotes CD8
T14993 28964-28973 Gene_expression denotes coexpress
T14994 28974-28977 Protein denotes CD4
T14995 29063-29068 Protein denotes Runx3
T14996 29134-29139 Protein denotes Runx3
T14997 29143-29146 Protein denotes CD8
T16116 29319-29322 Protein denotes CD8
T16117 29477-29486 Protein denotes Annexin V
T16118 29501-29504 Protein denotes CD8
T16119 29608-29611 Protein denotes CD8
T16120 29663-29672 Protein denotes Annexin V
T16643 30624-30627 Protein denotes CD8
T16644 30931-30941 Protein denotes Eomes-VP16
T16938 31099-31102 Protein denotes CD8
T16939 32123-32128 Protein denotes Eomes
T16940 32150-32155 Protein denotes Runx3
T17558 34389-34392 Protein denotes CD8
T17559 34482-34486 Protein denotes IL-2
T18115 35102-35105 Protein denotes CD8
T18116 35120-35125 Protein denotes Runx3
T18117 35180-35185 Protein denotes Runx3
T18308 6581-6584 Protein denotes CD8 true
T18309 6626-6630 Protein denotes Prf1 true
T18310 6632-6636 Protein denotes Gzmb true
T18311 6638-6643 Protein denotes Tbx21 true
T18312 6645-6650 Protein denotes T-bet true
T18313 6657-6662 Protein denotes Eomes true
T18314 6663-6678 Transcription denotes mRNA expression true
T18315 6702-6705 Protein denotes CD8 true
T18316 6965-6968 Protein denotes CD8
T18317 7126-7134 Gene_expression denotes staining
T18318 7139-7149 Protein denotes granzyme B
T18319 7151-7156 Protein denotes IFN-γ
T18320 7162-7165 Protein denotes TNF
T18321 7167-7177 Protein denotes Granzyme B
T18322 7361-7364 Protein denotes CD8
T18323 7485-7494 Protein denotes Annexin V
T18324 7528-7531 Protein denotes CD8
T18325 7730-7738 Protein denotes perforin
T18326 7739-7749 Protein denotes granzyme B
T19110 11884-11894 Regulation denotes Regulation
T19111 11898-11906 Protein denotes perforin
T19112 11908-11918 Protein denotes granzyme B
T19113 11924-11929 Protein denotes IFN-γ
T19114 11930-11940 Gene_expression denotes expression
T19115 11944-11949 Protein denotes T-bet
T19116 11954-11959 Protein denotes Eomes
T19117 11989-11994 Protein denotes IFN-γ
T19118 11995-12005 Gene_expression denotes expression
T19119 12013-12018 Protein denotes Tbx21 true
T19120 12027-12032 Protein denotes T-bet
T19121 12033-12042 Negative_regulation denotes deficient true
T19122 12044-12049 Protein denotes Tbx21 true
T19123 12069-12072 Protein denotes CD8 true
T19124 12183-12188 Protein denotes IFN-γ
T19125 12189-12199 Gene_expression denotes expression
T19126 12271-12276 Protein denotes IFN-γ true
T19127 12315-12319 Protein denotes Prf1 true
T19128 12324-12328 Protein denotes GzmB true
T19129 12329-12344 Transcription denotes mRNA expression
T19130 12354-12359 Protein denotes T-bet
T19131 12360-12369 Negative_regulation denotes deficient
T19132 12370-12373 Protein denotes CD8
T19133 12460-12470 Gene_expression denotes expressing
T19134 12471-12481 Protein denotes Eomes-VP16
T19135 12483-12490 Protein denotes Eo-VP16
T19136 12509-12512 Protein denotes GFP
T19137 12707-12717 Protein denotes Granzyme B
T19138 12722-12727 Protein denotes IFN-γ
T19139 12728-12738 Gene_expression denotes expression
T19140 12742-12747 Protein denotes Tbx21
T19141 12755-12760 Protein denotes Tbx21
T19142 12839-12844 Protein denotes IFN-γ
T19143 12845-12855 Gene_expression denotes production
T19144 12865-12875 Positive_regulation denotes transduced
T19145 12881-12888 Protein denotes Eo-VP16
T19146 12901-12904 Protein denotes GFP
T19147 13024-13027 Protein denotes GFP
T19148 13029-13034 Protein denotes IFN-γ
T20100 15071-15076 Protein denotes Runx3 true
T20101 15134-15139 Protein denotes Runx3 true
T20102 15141-15146 Protein denotes Eomes true
T20103 15148-15153 Protein denotes T-bet true
T20104 15159-15167 Protein denotes perforin true
T20105 15168-15178 Gene_expression denotes expression true
T20106 15182-15187 Protein denotes Runx3 true
T20107 15198-15203 Protein denotes Runx3 true
T20108 15207-15210 Protein denotes CD8 true
T20109 15247-15254 Protein denotes β-Actin true
T20110 15316-15320 Protein denotes Prf1 true
T20111 15321-15336 Transcription denotes mRNA expression
T20112 15340-15345 Protein denotes Runx3
T20113 15356-15361 Protein denotes Runx3
T20114 15365-15368 Protein denotes CD8
T20115 15402-15409 Protein denotes β–Actin
T20116 15445-15455 Gene_expression denotes Expression
T20117 15459-15469 Protein denotes granzyme B
T20118 15471-15476 Protein denotes IFN-γ
T20119 15478-15481 Protein denotes TNF
T20120 15487-15491 Protein denotes IL-2
T20121 15525-15530 Protein denotes Runx3
T20122 15541-15546 Protein denotes Runx3
T20123 15550-15553 Protein denotes CD8
T20124 15627-15637 Protein denotes granzyme B
T20125 15649-15652 Protein denotes GFP
T20126 15748-15755 Binding denotes binding
T20127 15770-15775 Protein denotes Runx3
T20128 15780-15785 Protein denotes Eomes
T20129 15793-15797 Protein denotes Prf1
T20130 16034-16037 Protein denotes CD8
T20131 16124-16128 Protein denotes Prf1
T20132 16147-16152 Protein denotes Runx3
T20133 16175-16180 Protein denotes Eomes
T20820 19206-19211 Protein denotes Runx3
T20821 19212-19220 Regulation denotes controls
T20822 19221-19226 Protein denotes Eomes
T20823 19228-19236 Protein denotes perforin
T20824 19238-19248 Protein denotes granzyme B
T20825 19254-19259 Protein denotes IFN-γ
T20826 19260-19270 Gene_expression denotes expression
T20827 19289-19294 Protein denotes Runx3
T20828 19301-19306 Protein denotes Runx3
T20829 19310-19313 Protein denotes CD8 true
T20830 19393-19396 Protein denotes GFP true
T20831 19429-19439 Protein denotes Eomes-VP16 true
T20832 19441-19448 Protein denotes Eo-VP16
T20833 19453-19456 Protein denotes Myc
T20834 19457-19462 Protein denotes Runx3
T20835 19464-19469 Protein denotes Runx3
T20836 19678-19692 Positive_regulation denotes Overexpression
T20837 19696-19706 Protein denotes Eomes-VP16
T20838 19735-19740 Protein denotes Eomes
T20839 19830-19834 Protein denotes VP16
T20840 19863-19873 Gene_expression denotes Expression
T20841 19877-19887 Protein denotes granzyme B
T20842 19892-19897 Protein denotes IFN-γ
T20843 20120-20130 Protein denotes granzyme B
T20844 20238-20241 Protein denotes GFP
T20845 20381-20386 Protein denotes Runx3 true
T20846 20406-20411 Protein denotes T-bet
T20847 20415-20422 Positive_regulation denotes induced true
T20848 20445-20454 Positive_regulation denotes essential
T20849 20465-20470 Protein denotes IFN-γ true
T20850 20471-20481 Gene_expression denotes expression true true
T20851 20483-20488 Protein denotes Runx3
T20852 20509-20512 Protein denotes CD8
T20853 20526-20535 Negative_regulation denotes represses
T20854 20536-20541 Protein denotes Runx1
T20855 20546-20553 Positive_regulation denotes induces true
T20856 20554-20559 Protein denotes Eomes
T20857 20561-20569 Protein denotes perforin
T20858 20571-20581 Protein denotes granzyme B
T20859 20587-20592 Protein denotes IFN-γ
T20860 20593-20603 Gene_expression denotes expression
T20861 20605-20610 Protein denotes Eomes
T20862 20630-20640 Positive_regulation denotes sustaining
T20863 20646-20651 Protein denotes IFN-γ
T20864 20652-20662 Gene_expression denotes expression
T20865 20672-20677 Protein denotes Runx3
T20866 20682-20687 Protein denotes Eomes
T20867 20697-20702 Protein denotes T-bet
T20868 20721-20729 Positive_regulation denotes activate
T20869 20730-20738 Protein denotes perforin
T20870 20739-20749 Gene_expression denotes expression
T20871 20799-20804 Protein denotes T-bet
T20872 20805-20815 Negative_regulation denotes deficiency
T20873 20819-20823 Protein denotes Gzmb
T20874 20837-20847 Protein denotes granzyme B
T20875 20856-20866 Gene_expression denotes expression
R602 T767 T769 themeOf Runx3,expressed
R603 T768 T769 themeOf perforin,expressed
R604 T772 T773 themeOf Runx3,expressed
R605 T772 T775 themeOf Runx3,expression
R606 T773 T776 themeOf expressed,up-regulated
R607 T775 T776 themeOf expression,up-regulated
R608 T777 T776 causeOf Runx3,up-regulated
R609 T777 T778 causeOf Runx3,regulation
R610 T779 T778 themeOf the Il4 and Ifng cytokine,regulation
R611 T782 T783 themeOf Runx3,deficient
R612 T787 T788 themeOf T-bet,induced
R613 T789 T790 themeOf Eomes,induced
R614 T789 T791 causeOf Eomes,sustains
R615 T792 T793 themeOf IFN-γ,expression
R616 T793 T791 themeOf expression,sustains
R617 T794 T795 causeOf Runx3,required
R618 T796 T798 themeOf Eomes,expression
R619 T797 T798 themeOf perforin,expression
R620 T798 T795 themeOf expression,required
R621 T799 T801 themeOf Eomes,bind
R622 T800 T801 themeOf Runx3,bind
R623 T801 T795 themeOf bind,required
R624 T802 T801 themeOf the Prf1 locus,bind
R625 T803 T804 themeOf perforin,expression
R626 T805 T806 themeOf T-bet,deficient
R627 T807 T809 causeOf lacking,decreased
R628 T808 T807 themeOf Runx3,lacking
R629 T808 T809 causeOf Runx3,decreased
R630 T810 T809 themeOf expression,decreased
R631 T811 T810 themeOf IFN-γ,expression
R632 T812 T810 themeOf granzyme B,expression
R633 T813 T810 themeOf Runx3,expression
R634 T813 T814 themeOf Runx3,binds
R635 T815 T816 partOf promoter regions,the Ifng and Gzmb genes
R636 T815 T814 themeOf promoter regions,binds
R637 T817 T818 causeOf Runx3,regulator
R638 T817 T822 causeOf Runx3,promote
R639 T819 T818 themeOf of Gzmb expression,regulator
R640 T820 T822 causeOf T-bet,promote
R641 T821 T822 causeOf Eomes,promote
R642 T823 T822 themeOf transcription,promote
R643 T824 T823 themeOf the Ifng and Prf1 genes,transcription
R2073 T2673 T2672 equivalentTo rhIL-2,IL-2
R2515 T3271 T3270 themeOf perforin,expression
R2516 T3272 T3270 themeOf granzyme B,expression
R2517 T3273 T3275 themeOf perforin,expression
R2518 T3274 T3275 themeOf granzyme B,expression
R2519 T3278 T3277 themeOf perforin,expression
R2520 T3279 T3277 themeOf perforin,expression
R2521 T3281 T3280 themeOf perforin,expression
R2522 T3281 T3283 themeOf perforin,mRNA expression
R2523 T3282 T3281 equivalentTo Prf1,perforin
R2524 T3283 T3285 themeOf mRNA expression,decrease
R2525 T3283 T3284 themeOf mRNA expression,increase
R2526 T3286 T3289 themeOf granzyme B,increased
R2527 T3286 T3288 themeOf granzyme B,up-regulated
R2528 T3287 T3286 equivalentTo Gzmb,granzyme B
R2529 T3287 T3288 themeOf Gzmb,up-regulated
R2530 T3288 T3289 themeOf up-regulated,increased
R2531 T3290 T3291 themeOf granzyme B,expressed
R2532 T3293 T3297 themeOf expressed,increased
R2533 T3294 T3293 themeOf IFN-γ,expressed
R2534 T3295 T3293 themeOf TNF,expressed
R2535 T3299 T3298 themeOf perforin,express
R2536 T3301 T3302 themeOf granzyme B,expression
R2537 T3303 T3304 themeOf perforin,expression
R2538 T3304 T3305 themeOf expression,induced
R3657 T4644 T4646 themeOf T-bet,expression
R3658 T4645 T4646 themeOf Eomes,expression
R3659 T4652 T4655 themeOf T-bet,expressed
R3660 T4652 T4654 themeOf T-bet,induced
R3661 T4656 T4657 themeOf Eomes,expression
R3662 T4656 T4659 themeOf Eomes,expression
R3663 T4661 T4660 themeOf Eomes,induction
R3664 T4662 T4663 themeOf T-bet,mRNA expression
R3665 T4665 T4664 themeOf expression,preceded
R3666 T4666 T4665 themeOf GzmB,expression
R3667 T4667 T4668 themeOf Eomes,expressed
R3668 T4670 T4669 themeOf perforin,reexpression
R3669 T4673 T4674 causeOf T-bet,required
R3670 T4675 T4676 themeOf IFN-γ,production
R3671 T4676 T4674 themeOf production,required
R3672 T4677 T4678 causeOf Eomes,contribute
R3673 T4680 T4681 themeOf perforin,expression
R3674 T4681 T4679 themeOf expression,control
R3675 T4683 T4682 themeOf T-bet,induce
R3676 T4683 T4684 causeOf T-bet,induces
R3677 T4685 T4684 themeOf IFN-γ,induces
R3678 T4686 T4684 themeOf granzyme B,induces
R3679 T4687 T4688 themeOf Eomes,induced
R3680 T4687 T4690 causeOf Eomes,activates
R3681 T4691 T4692 themeOf perforin,expression
R3682 T4692 T4690 themeOf expression,activates
R4725 T5971 T5973 themeOf Perforin,expression
R4726 T5972 T5973 themeOf granzyme B,expression
R4727 T5973 T5974 themeOf expression,regulated
R4728 T5976 T5975 themeOf IFN-γ,expression
R4729 T5977 T5975 themeOf perforin,expression
R4730 T5978 T5975 themeOf granzyme B,expression
R4731 T5980 T5982 themeOf Tbx21,deficient
R4732 T5981 T5980 equivalentTo T-bet,Tbx21
R4733 T5986 T5985 themeOf IFN-γ,produced
R4734 T5987 T5988 themeOf T-bet,deficiency
R4735 T5991 T5992 themeOf Eomes,induced
R4736 T5993 T5994 themeOf T-bet,deficient
R4737 T5994 T5995 causeOf deficient,defect
R4738 T5996 T5997 themeOf perforin,mRNA expression
R4739 T5997 T5995 themeOf mRNA expression,defect
R4740 T5999 T5998 themeOf GzmB,reduction
R4741 T6000 T6001 themeOf T-bet,deficient
R4742 T6001 T5998 causeOf deficient,reduction
R4743 T6003 T6002 themeOf expression,decrease
R4744 T6004 T6003 themeOf granzyme B,expression
R4745 T6010 T6009 equivalentTo Eo-VP16,Eomes
R4746 T6011 T6013 causeOf Eo-VP16,increased
R4747 T6014 T6015 themeOf perforin,expression
R4748 T6015 T6013 themeOf expression,increased
R4749 T6016 T6017 themeOf T-bet,deficient
R4750 T6019 T6020 causeOf Eo-VP16,defect
R4751 T6021 T6022 themeOf IFN-γ,production
R4752 T6022 T6020 themeOf production,defect
R4753 T6023 T6024 themeOf T-bet,deficient
R4754 T6024 T6020 causeOf deficient,defect
R4755 T6026 T6027 causeOf Eo-VP16,induce
R4756 T6028 T6029 themeOf GzmB,mRNA expression
R4757 T6029 T6027 themeOf mRNA expression,induce
R4758 T6030 T6031 themeOf T-bet,deficient
R4759 T6031 T6027 causeOf deficient,induce
R4760 T6032 T6033 causeOf T-bet,dependence
R4761 T6034 T6035 themeOf GzmB,mRNA expression
R4762 T6035 T6033 themeOf mRNA expression,dependence
R5933 T7587 T7586 themeOf Eomes,induction
R5934 T7588 T7589 themeOf Runx3,expressed
R5935 T7602 T7601 themeOf perforin,expression
R5936 T7603 T7601 themeOf granzyme B,expression
R5937 T7604 T7601 themeOf IFN-γ,expression
R5938 T7605 T7606 themeOf perforin,expression
R5939 T7609 T7610 themeOf Eomes,expression
R5940 T7611 T7612 themeOf T-bet,expression
R5941 T7612 T7613 themeOf expression,unimpaired
R5942 T7614 T7615 causeOf Runx3,required
R5943 T7616 T7615 themeOf production,required
R5944 T7617 T7616 themeOf IFN-γ,production
R5945 T7618 T7616 themeOf TNF,production
R5946 T7619 T7616 themeOf IL-2,production
R5947 T7621 T7622 themeOf T-bet,up-regulated
R5948 T7624 T7623 themeOf Runx3,induces
R5949 T7625 T7627 causeOf T-bet,induce
R5950 T7625 T7629 causeOf T-bet,silence
R5951 T7626 T7627 causeOf Runx3,induce
R5952 T7626 T7629 causeOf Runx3,silence
R5953 T7628 T7627 themeOf IFN-γ,induce
R5954 T7630 T7629 themeOf IL-4,silence
R5955 T7631 T7632 causeOf Runx3,necessary
R5956 T7633 T7634 themeOf Eomes,induction
R5957 T7634 T7632 themeOf induction,necessary
R5958 T7635 T7636 themeOf Eomes,expression
R5959 T7637 T7638 themeOf perforin,expression
R5960 T7640 T7639 themeOf Eo-VP16,overexpression
R5961 T7641 T7642 themeOf T-bet,deficient
R5962 T7644 T7646 themeOf perforin,expression
R5963 T7645 T7646 themeOf IFN-γ,expression
R5964 T7646 T7643 themeOf expression,increase
R5965 T7647 T7648 causeOf Runx3,necessary
R5966 T7647 T7652 causeOf Runx3,regulate
R5967 T7649 T7650 themeOf Eomes,induction
R5968 T7650 T7648 themeOf induction,necessary
R5969 T7651 T7652 causeOf Eomes,regulate
R5970 T7653 T7652 themeOf transcription,regulate
R5971 T7654 T7653 themeOf perforin,transcription
R5972 T7655 T7653 themeOf IFN-γ,transcription
R5973 T7656 T7653 themeOf granzyme B,transcription
R5974 T7657 T7659 themeOf Eomes,bound
R5975 T7658 T7659 themeOf Runx3,bound
R5976 T7660 T7659 themeOf regulatory regions,bound
R5977 T7660 T7661 partOf regulatory regions,Prf1
R5978 T7660 T7662 partOf regulatory regions,Ifng
R5979 T7660 T7663 partOf regulatory regions,Gzmb
R5980 T7664 T7665 themeOf Runx3,bound
R5981 T7668 T7666 partOf transcription start sites,Prf1
R5982 T7668 T7667 partOf transcription start sites,Gzmb
R5983 T7668 T7665 themeOf transcription start sites,bound
R5984 T7673 T7674 themeOf Eomes,bound
R5985 T7676 T7674 themeOf TSS,bound
R5986 T7676 T7675 partOf TSS,Prf1
R5987 T7677 T7674 themeOf −1 kb enhancer,bound
R5988 T7677 T7675 partOf −1 kb enhancer,Prf1
R5989 T7679 T7682 partOf promoter,Eomes
R5990 T7679 T7681 themeOf promoter,target
R5991 T7679 T7680 partOf promoter,Il2rb
R5992 T7682 T7681 themeOf Eomes,target
R5993 T7684 T7683 partOf TSS,Ifng
R5994 T7686 T7687 causeOf Runx3,induction
R5995 T7688 T7687 themeOf Eomes,induction
R5996 T7690 T7689 themeOf Runx3,expressed
R5997 T7691 T7689 themeOf Eo-VP16,expressed
R5998 T7706 T7705 themeOf Eomes,expression
R5999 T7707 T7705 themeOf perforin,expression
R6000 T7708 T7705 themeOf granzyme B,expression
R6001 T7709 T7705 themeOf IFN-γ,expression
R6002 T7711 T7713 themeOf up-regulation,suppressed
R6003 T7712 T7711 themeOf Runx1,up-regulation
R6004 T7714 T7713 causeOf Runx3,suppressed
R6005 T7715 T7716 themeOf Runx1,repression
R6006 T7717 T7716 causeOf Runx3,repression
R6007 T7718 T7722 themeOf Eo-VP16,expressed
R6008 T7718 T7719 causeOf Eo-VP16,up-regulate
R6009 T7720 T7721 themeOf perforin,expression
R6010 T7721 T7719 themeOf expression,up-regulate
R6011 T7725 T7726 themeOf IFN-γ,expression
R6012 T7726 T7724 themeOf expression,induce
R6013 T7727 T7728 themeOf perforin,expression
R6014 T7728 T7729 themeOf expression,requires
R6015 T7730 T7729 causeOf Runx3,requires
R6016 T7731 T7729 causeOf Eomes,requires
R6017 T7733 T7735 themeOf perforin,expression
R6018 T7734 T7735 themeOf granzyme B,expression
R6019 T7735 T7732 themeOf expression,defect
R6020 T7742 T7745 themeOf activation,defective
R6021 T7743 T7742 themeOf perforin,activation
R6022 T7744 T7742 themeOf granzyme B,activation
R6023 T7748 T7745 causeOf Fas,defective
R6024 T7749 T7748 themeOf Fas ligand,Fas
R9084 T11600 T11599 themeOf Runx1,represses
R9085 T11600 T11601 causeOf Runx1,has a positive role
R9086 T11602 T11601 themeOf induction,has a positive role
R9087 T11603 T11602 themeOf Eomes,induction
R9088 T11604 T11602 themeOf granzyme B,induction
R9089 T11605 T11602 themeOf perforin,induction
R9090 T11606 T11602 themeOf IFN-γ,induction
R9091 T11607 T11608 themeOf Runx3,binds
R9092 T11609 T11608 themeOf promoters,binds
R9093 T11610 T11608 themeOf regulatory regions,binds
R9094 T11614 T11615 causeOf Runx3,contributed
R9095 T11616 T11615 themeOf expression,contributed
R9096 T11617 T11616 themeOf TNF,expression
R9097 T11618 T11616 themeOf IL-2,expression
R9098 T11619 T11616 themeOf IFN-γ,expression
R9099 T11625 T11626 causeOf Runx3,required
R9100 T11625 T11629 causeOf Runx3,role
R9101 T11627 T11628 themeOf IFN-γ,expression
R9102 T11628 T11626 themeOf expression,required
R9103 T11631 T11632 themeOf Eomes,expression
R9104 T11632 T11630 themeOf expression,induction
R9105 T11633 T11635 themeOf T-bet,up-regulated
R9106 T11634 T11635 themeOf Eomes,up-regulated
R9107 T11640 T11639 themeOf IFN-γ,produce
R9108 T11641 T11642 themeOf Eomes,induced
R9109 T11641 T11646 causeOf Eomes,promoting
R9110 T11645 T11646 causeOf T-bet,promoting
R9111 T11647 T11646 themeOf expression,promoting
R9112 T11648 T11647 themeOf IFN-γ,expression
R9113 T11649 T11651 causeOf T-bet,contribute
R9114 T11650 T11651 causeOf Eomes,contribute
R9115 T11652 T11653 themeOf perforin,expression
R9116 T11653 T11651 themeOf expression,contribute
R9117 T11654 T11655 causeOf Eomes,induces
R9118 T11656 T11655 themeOf granzyme B,induces
R9119 T11658 T11660 causeOf Eomes,induce
R9120 T11659 T11660 causeOf T-bet,induce
R9121 T11661 T11660 themeOf Prf1,induce
R9122 T11662 T11660 themeOf Gzmb,induce
R9123 T11663 T11665 causeOf T-bet,regulating
R9124 T11664 T11665 causeOf Eomes,regulating
R9125 T11666 T11668 themeOf granzyme B,expression
R9126 T11667 T11668 themeOf perforin,expression
R9127 T11668 T11665 themeOf expression,regulating
R9128 T11670 T11669 themeOf T-bet,up-regulation
R9129 T11671 T11669 themeOf Eomes,up-regulation
R9130 T11673 T11672 themeOf Gzmb,up-regulation
R9131 T11674 T11672 themeOf Prf1,up-regulation
R9132 T11675 T11683 themeOf T-bet,expressed
R9133 T11675 T11676 causeOf T-bet,role
R9134 T11677 T11678 themeOf perforin,expression
R9136 T11679 T11683 themeOf Eo-VP16,expressed
R9137 T11679 T11680 causeOf Eo-VP16,affect
R9138 T11681 T11682 themeOf granzyme B,expression
R9139 T11682 T11680 themeOf expression,affect
R11595 T14953 T14954 themeOf Runx3,deficient
R11596 T14954 T14955 causeOf deficient,silence
R11597 T14956 T14957 themeOf CD4,expression
R11598 T14957 T14955 themeOf expression,silence
R11599 T14976 T14977 themeOf CD4,expression
R11600 T14977 T14975 themeOf expression,impaired
R11601 T14980 T14979 themeOf CD25,up-regulation
R11602 T14981 T14979 themeOf CD69,up-regulation
R11603 T14983 T14982 themeOf CD25,up-regulate
R11604 T14986 T14987 themeOf IL-2,supplementation
R11605 T14989 T14990 themeOf CD4,expression
R11606 T14990 T14988 themeOf expression,silenced
R11607 T14994 T14993 themeOf CD4,coexpress
R14219 T18309 T18314 themeOf Prf1,mRNA expression
R14220 T18310 T18314 themeOf Gzmb,mRNA expression
R14221 T18311 T18314 themeOf Tbx21,mRNA expression
R14222 T18312 T18311 equivalentTo T-bet,Tbx21
R14223 T18313 T18314 themeOf Eomes,mRNA expression
R14224 T18318 T18317 themeOf granzyme B,staining
R14225 T18319 T18317 themeOf IFN-γ,staining
R14226 T18320 T18317 themeOf TNF,staining
R14816 T19111 T19114 themeOf perforin,expression
R14817 T19112 T19114 themeOf granzyme B,expression
R14818 T19113 T19114 themeOf IFN-γ,expression
R14819 T19114 T19110 themeOf expression,Regulation
R14820 T19115 T19110 causeOf T-bet,Regulation
R14821 T19116 T19110 causeOf Eomes,Regulation
R14822 T19117 T19118 themeOf IFN-γ,expression
R14823 T19120 T19121 themeOf T-bet,deficient
R14824 T19124 T19125 themeOf IFN-γ,expression
R14825 T19127 T19129 themeOf Prf1,mRNA expression
R14826 T19128 T19129 themeOf GzmB,mRNA expression
R14827 T19130 T19131 themeOf T-bet,deficient
R14828 T19134 T19133 themeOf Eomes-VP16,expressing
R14829 T19135 T19134 equivalentTo Eo-VP16,Eomes-VP16
R14830 T19137 T19139 themeOf Granzyme B,expression
R14831 T19138 T19139 themeOf IFN-γ,expression
R14832 T19142 T19143 themeOf IFN-γ,production
R14833 T19145 T19144 themeOf Eo-VP16,transduced
R15528 T20101 T20105 themeOf Runx3,expression
R15529 T20102 T20105 themeOf Eomes,expression
R15530 T20103 T20105 themeOf T-bet,expression
R15531 T20104 T20105 themeOf perforin,expression
R15532 T20110 T20111 themeOf Prf1,mRNA expression
R15533 T20117 T20116 themeOf granzyme B,Expression
R15534 T20118 T20116 themeOf IFN-γ,Expression
R15535 T20119 T20116 themeOf TNF,Expression
R15536 T20120 T20116 themeOf IL-2,Expression
R15537 T20127 T20126 themeOf Runx3,binding
R15538 T20128 T20126 themeOf Eomes,binding
R15539 T20129 T20126 themeOf Prf1,binding
R15933 T20820 T20821 causeOf Runx3,controls
R15934 T20822 T20826 themeOf Eomes,expression
R15935 T20823 T20826 themeOf perforin,expression
R15936 T20824 T20826 themeOf granzyme B,expression
R15937 T20825 T20826 themeOf IFN-γ,expression
R15938 T20826 T20821 themeOf expression,controls
R15939 T20832 T20831 equivalentTo Eo-VP16,Eomes-VP16
R15940 T20837 T20836 themeOf Eomes-VP16,Overexpression
R15941 T20841 T20840 themeOf granzyme B,Expression
R15942 T20842 T20840 themeOf IFN-γ,Expression
R15943 T20846 T20848 causeOf T-bet,essential
R15944 T20846 T20847 themeOf T-bet,induced
R15945 T20849 T20850 themeOf IFN-γ,expression
R15946 T20850 T20848 themeOf expression,essential
R15947 T20854 T20855 causeOf Runx1,induces
R15948 T20856 T20860 themeOf Eomes,expression
R15949 T20857 T20860 themeOf perforin,expression
R15950 T20858 T20860 themeOf granzyme B,expression
R15951 T20859 T20860 themeOf IFN-γ,expression
R15952 T20860 T20855 themeOf expression,induces
R15953 T20861 T20862 causeOf Eomes,sustaining
R15954 T20861 T20868 causeOf Eomes,activate
R15955 T20863 T20864 themeOf IFN-γ,expression
R15956 T20864 T20862 themeOf expression,sustaining
R15957 T20865 T20868 causeOf Runx3,activate
R15958 T20866 T20868 causeOf Eomes,activate
R15959 T20867 T20868 causeOf T-bet,activate
R15960 T20869 T20870 themeOf perforin,expression
R15961 T20870 T20868 themeOf expression,activate
R15962 T20871 T20872 themeOf T-bet,deficiency
R15963 T20873 T20875 themeOf Gzmb,expression
R15964 T20874 T20875 themeOf granzyme B,expression
R435 T33 T37 causeOf T-bet,regulate
R436 T33 T32 themeOf T-bet,induction
R437 T35 T34 themeOf Eomesodermin,induction
R438 T35 T37 causeOf Eomesodermin,regulate
R439 T36 T35 equivalentTo Eomes,Eomesodermin
R440 T38 T39 themeOf interferon (IFN) γ,expression
R441 T39 T37 themeOf expression,regulate
R442 T41 T42 causeOf Runx3,regulates
R443 T43 T44 themeOf Eomes,expression
R444 T44 T42 themeOf expression,regulates
R445 T46 T45 themeOf IFN-γ,expression
R446 T47 T45 themeOf perforin,expression
R447 T48 T45 themeOf granzyme B,expression
R593 T746 T745 themeOf IFN-γ,produce
R594 T747 T745 themeOf TNF,produce
R595 T750 T749 equivalentTo Eomes,Eomesodermin
R596 T753 T752 themeOf the Tbx21,deletion
R597 T754 T753 equivalentTo T-bet,the Tbx21
R598 T755 T756 themeOf and eomesodermin genes,associated
R599 T755 T752 themeOf and eomesodermin genes,deletion
R600 T760 T759 themeOf T-bet,deficient
R601 T761 T759 themeOf Eomes,deficient
R9135 T11678 T11676 themeOf expression,role
R9140 T11686 T11687 themeOf Eomes,deficient
R9141 T11689 T11688 themeOf Eomes,deficient
R9142 T11691 T11692 themeOf Eomes,deficient
R9143 T11694 T11696 causeOf Eomes,induce
R9144 T11695 T11696 causeOf Runx3,induce
R9145 T11697 T11698 themeOf perforin,expression
R9146 T11698 T11696 themeOf expression,induce
R9147 T11701 T11700 themeOf T-bet,induced
R9148 T11702 T11700 themeOf Eomes,induced
R9149 T11703 T11700 themeOf IL-2Rβ,induced
R9150 T11705 T11704 causeOf T-bet,interaction
R9151 T11706 T11704 causeOf Runx3,interaction
R9152 T11708 T11709 themeOf T-bet,induced
R9153 T11708 T11711 causeOf T-bet,induces
R9154 T11710 T11709 causeOf IFN-γ,induced
R9155 T11712 T11711 themeOf Runx3,induces
R9156 T11714 T11715 causeOf Runx3,required
R9157 T11716 T11715 themeOf induce,required
R9158 T11717 T11716 themeOf Eomes,induce