PMC:2586094 / 9756-11302
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2586094","sourcedb":"PMC","sourceid":"2586094","source_url":"http://www.ncbi.nlm.nih.gov/pmc/2586094","text":"3.2 The action of IFN-γ on the synthesis and activity of CK2\nCK2 exists as a tetramer with two main catalytic (α and α′) and two regulatory β subunits as well as free catalytic subunits [10,22]. In order to investigate whether IFN-γ affects the synthesis of CK2, the levels of the α and α′ polypeptides in J774.2 cells treated with IFN-γ were determined by western blot analysis. Sp1 and Sp3 were also analysed for comparative purposes (Sp1 can also act as a loading control as IFN-γ has no effect on its expression [9]). Fig. 2A shows that IFN-γ does not affect the steady state levels of CK2-α and -α′ polypeptides. In contrast, consistent with our previous studies [9], IFN-γ reduced the polypeptide levels of Sp3 but not Sp1 (Fig. 2A).\nOur previous studies on the action of IFN-γ on CK2 activation, which was restricted to the α isoform and a single time point (3 h) [10], showed a dramatic increase in activity following stimulation of the cells with this cytokine. Preliminary time course experiments showed that the activity of both CK2 catalytic subunits was induced within 1 h of incubation of the cells with IFN-γ, peaked at 3 h and was sustained, albeit at reduced levels, for 12–20 h (data not shown). In order to confirm the inhibitory action of apigenin, its effect on the IFN-γ-induced activity of both the catalytic subunits at 3 h was determined. Fig. 2B shows that, consistent with previous studies [e.g. [10,16–19]], apigenin indeed abolishes CK2 activity of both subunits without affecting the expression of the polypeptides.","divisions":[{"label":"Title","span":{"begin":5,"end":61}}],"tracks":[{"project":"2_test","denotations":[{"id":"18793716-12609974-20139059","span":{"begin":191,"end":193},"obj":"12609974"},{"id":"18793716-18437331-20139059","span":{"begin":191,"end":193},"obj":"18437331"},{"id":"18793716-11796707-20139060","span":{"begin":518,"end":519},"obj":"11796707"},{"id":"18793716-11796707-20139061","span":{"begin":670,"end":671},"obj":"11796707"},{"id":"18793716-12609974-20139062","span":{"begin":873,"end":875},"obj":"12609974"},{"id":"18793716-12609974-20139063","span":{"begin":1431,"end":1433},"obj":"12609974"},{"id":"18793716-12149649-20139063","span":{"begin":1431,"end":1433},"obj":"12149649"},{"id":"18793716-12738992-20139063","span":{"begin":1431,"end":1433},"obj":"12738992"},{"id":"18793716-15688023-20139063","span":{"begin":1431,"end":1433},"obj":"15688023"},{"id":"18793716-14993215-20139063","span":{"begin":1431,"end":1433},"obj":"14993215"}],"attributes":[{"subj":"18793716-12609974-20139059","pred":"source","obj":"2_test"},{"subj":"18793716-18437331-20139059","pred":"source","obj":"2_test"},{"subj":"18793716-11796707-20139060","pred":"source","obj":"2_test"},{"subj":"18793716-11796707-20139061","pred":"source","obj":"2_test"},{"subj":"18793716-12609974-20139062","pred":"source","obj":"2_test"},{"subj":"18793716-12609974-20139063","pred":"source","obj":"2_test"},{"subj":"18793716-12149649-20139063","pred":"source","obj":"2_test"},{"subj":"18793716-12738992-20139063","pred":"source","obj":"2_test"},{"subj":"18793716-15688023-20139063","pred":"source","obj":"2_test"},{"subj":"18793716-14993215-20139063","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93aa","default":true}]}]}}