PMC:2586094 / 5095-5743
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2586094","sourcedb":"PMC","sourceid":"2586094","source_url":"http://www.ncbi.nlm.nih.gov/pmc/2586094","text":"2.2 Cell culture\nCells were maintained in Dulbecco's modified Eagle's medium (J744.2) or RPMI 1640 (U937) supplemented with heat-inactivated (56 °C, 30 min) foetal calf serum, 100 U/ml penicillin and 100 µg/ml streptomycin. The cultures were maintained at 37 °C in a humidified atmosphere containing 5% (v/v) CO2 in air. Treatment of J774.2 macrophages with IFN-γ (1000 U/ml) or the different pharmacological inhibitors was performed as previously described [9–14]. U937 cells were used for all transfection assays where differentiation was initiated using 1 µM phorbol-12-myristate-13-acetate (PMA) and monitored by morphological analysis [9,15].","divisions":[{"label":"Title","span":{"begin":5,"end":17}}],"tracks":[{"project":"2_test","denotations":[{"id":"18793716-11796707-20139048","span":{"begin":462,"end":464},"obj":"11796707"},{"id":"18793716-12609974-20139048","span":{"begin":462,"end":464},"obj":"12609974"},{"id":"18793716-17255531-20139048","span":{"begin":462,"end":464},"obj":"17255531"},{"id":"18793716-8891433-20139048","span":{"begin":462,"end":464},"obj":"8891433"},{"id":"18793716-9505144-20139048","span":{"begin":462,"end":464},"obj":"9505144"},{"id":"18793716-10419646-20139048","span":{"begin":462,"end":464},"obj":"10419646"},{"id":"18793716-11796707-20139049","span":{"begin":644,"end":646},"obj":"11796707"},{"id":"18793716-15755745-20139049","span":{"begin":644,"end":646},"obj":"15755745"}],"attributes":[{"subj":"18793716-11796707-20139048","pred":"source","obj":"2_test"},{"subj":"18793716-12609974-20139048","pred":"source","obj":"2_test"},{"subj":"18793716-17255531-20139048","pred":"source","obj":"2_test"},{"subj":"18793716-8891433-20139048","pred":"source","obj":"2_test"},{"subj":"18793716-9505144-20139048","pred":"source","obj":"2_test"},{"subj":"18793716-10419646-20139048","pred":"source","obj":"2_test"},{"subj":"18793716-11796707-20139049","pred":"source","obj":"2_test"},{"subj":"18793716-15755745-20139049","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#bc93ec","default":true}]}]}}