PMC:2518050 / 8551-9495 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2518050","sourcedb":"PMC","sourceid":"2518050","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2518050","text":"Adult mice (P42) were transcardially perfused with 4% PFA. Brain sections (50–100 μm) were pre-incubated in 5% normal goat serum (NGS; Sigma, St. Louis, MO) for 2 hours at room temperature and transferred to polyclonal Homer1a and GFP antibody solution containing 0.5% Triton X-100 and 1% NGS in PBS. Sections were incubated in primary antibody solution for 48 hours at 4 °C, washed 10 minutes in PBS, and incubated in biotinylated secondary antibody goat anti-rabbit IgG 1:500 (Vector; Burlingame, CA) for 2 hours at room temperature. After washing in PBS (10 minutes), the sections were incubated in streptavidin–biotinperoxidase complex (ABC standard kit, Vector) for 1–2 hours at room temperature. Immunolabeling was revealed by the glucose oxidase–diaminobenzidine (GOD-DAB) method. Vibratome sections were washed in PBS, mounted on Superfrost glass slides (Menzel; Braunschweig, Germany), dried, dehydrated, and cover-slipped with xylene.","tracks":[]}