PMC:2495064 / 8123-8802 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2495064","sourcedb":"PMC","sourceid":"2495064","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2495064","text":"The mitochondrial noncoding control region (D-loop) was sequenced by PCR of four overlapping segments, with the use of forward and reverse M13-tagged primer pairs. Primer sequences are shown in Table S3. PCR products were treated with ExoSAP (ExoSAP-IT, GE Healthcare, USA) and sequenced on a fluorescent genetic analyzer (Beckman-Coulter CEQ 8000) with the standard dideoxy chain-termination method (Beckman-Coulter Quickstart). The sequence data were compared to the revised Cambridge reference sequence (rCRS)16 with the use of CEQ Sequence Analysis v2.3.13 analysis software (Beckman-Coulter). This allowed identification of D-loop changes and comparison of positive samples.","tracks":[{"project":"2_test","denotations":[{"id":"18674747-10508508-2049171","span":{"begin":512,"end":514},"obj":"10508508"}],"attributes":[{"subj":"18674747-10508508-2049171","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#aa93ec","default":true}]}]}}