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Id Subject Object Predicate Lexical cue
T353 0-22 Sentence denotes Supporting Information
T354 23-78 Sentence denotes Figure S1 Phenotype of In Vitro Differentiated T Cells
T355 79-284 Sentence denotes After two round of differentiation cultures, T cells were stimulated by plate-immobilized anti-CD3/CD28 and 3H-thymidine incorporation as measurement of proliferation was analyzed after 3 d of culture (A).
T356 285-397 Sentence denotes In parallel, T cells were analyzed for Treg relevant surface receptor expression as indicated on the x-axis (B).
T357 398-410 Sentence denotes (1.0 MB AI).
T358 411-447 Sentence denotes Click here for additional data file.
T359 450-524 Sentence denotes Figure S2 In Vivo Treatment of Mice with IL-4 Antibody–Cytokine Complexes
T360 525-753 Sentence denotes B6 mice were given every other day ip injections of phosphate-buffered saline (PBS), recombinant mouse IL-4 (rmIL-4), anti-IL-4 mAb (anti-IL-4 mAb, 11B11, or MAB404), or a mixture of rmIL-4 plus anti-IL-4 mAbs (11B11 or MAB404).
T361 754-834 Sentence denotes Mice were analyzed on day 7 by flow cytometry for CD3, CD4, and CD25 expression.
T362 835-903 Sentence denotes Shown is CD25 versus CD4 expression in CD3+ CD4+ spleen cells (A–F).
T363 904-961 Sentence denotes Numbers indicate percentages of CD4+ CD25high CD3+ cells.
T364 962-1059 Sentence denotes Total cell counts (G) of CD4+ CD25high cells in spleen from mice in (A–F) are shown as mean ± SD.
T365 1060-1121 Sentence denotes The data are representative of three independent experiments.
T366 1122-1134 Sentence denotes (369 KB AI).
T367 1135-1171 Sentence denotes Click here for additional data file.
T368 1174-1251 Sentence denotes Figure S3 Effect of IL-4 on Already Existing Natural or Inducible Treg Cells
T369 1252-1456 Sentence denotes (A) CD4+CD25high nTreg cells were FACS-sorted and activated with plate-bound anti-CD3/CD28 plus IL-2 during 3 d and in the presence or absence of IL-4 (100 ng/ml) and harvested for real-time PCR analysis.
T370 1457-1532 Sentence denotes The results shown represent the mean ± SD of three independent experiments.
T371 1533-1571 Sentence denotes (B) iTreg cells were induced in vitro.
T372 1572-1820 Sentence denotes FOXP3 espression was assessed by real-time PCR analysis in resting cells, in cells re-stimulated with plate-bound anti-CD3/CD28, with or without TGF-β, plus IL-2 during 3 d and in the presence (black bar) or absence (white bar) of IL-4 (100 ng/ml).
T373 1821-1926 Sentence denotes (C) Activation dramatically increases CD4+CD25+ Treg cells suppressive capacity of CD4+CD25+ nTreg cells.
T374 1927-2041 Sentence denotes CD4+CD25+ nTreg cells were preactivated during 2 d in the presence or absence of an increasing IL-4 concentration.
T375 2042-2127 Sentence denotes After vigorous washing, their suppressive capacity on responder CD4+CD25− was tested.
T376 2128-2220 Sentence denotes IL-4 pretreatment did not affect the suppressive capacity of FACS-sorted CD4+CD25high cells.
T377 2221-2312 Sentence denotes 1 × 104 CD4+CD25+ nTreg cells were added to 5 × 104 CD4+CD25– and 5 × 104 irradiated PBMCs.
T378 2313-2377 Sentence denotes The results are representative of three independent experiments.
T379 2378-2390 Sentence denotes (269 KB AI).
T380 2391-2427 Sentence denotes Click here for additional data file.
T381 2430-2510 Sentence denotes Figure S4 Schematic Structure of the FOXP3 Gene and Location of the GATA3 Sites
T382 2511-2629 Sentence denotes The scheme shows the location of the 11 exons spaced by a large intron (6000 bp) from the 5′untranslated region (UTR).
T383 2630-2737 Sentence denotes Human, murine, and rat sequences are aligned and transcription start site (TSS) is indicated with an arrow.
T384 2738-2750 Sentence denotes (529 KB AI).
T385 2751-2787 Sentence denotes Click here for additional data file.