PMC:2222968 / 43528-43831
Annnotations
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21842 | 0-10 | JJ | denotes | Unspecific |
T21843 | 11-18 | NN | denotes | binding |
T21844 | 19-22 | VB | denotes | was |
T21845 | 23-30 | VB | denotes | blocked |
T21846 | 31-35 | IN | denotes | with |
T21847 | 36-39 | NN | denotes | BSA |
T21848 | 39-40 | -COMMA- | denotes | , |
T21849 | 41-44 | CC | denotes | and |
T21850 | 45-48 | DT | denotes | the |
T21851 | 49-58 | NN | denotes | membranes |
T21852 | 59-63 | VB | denotes | were |
T21853 | 64-76 | RB | denotes | subsequently |
T21854 | 77-86 | VB | denotes | incubated |
T21855 | 87-91 | IN | denotes | with |
T21856 | 92-94 | DT | denotes | an |
T21857 | 95-96 | CD | denotes | 1 |
T21858 | 96-97 | -COLON- | denotes | : |
T21859 | 97-100 | CD | denotes | 200 |
T21860 | 101-109 | NN | denotes | dilution |
T21861 | 110-112 | IN | denotes | of |
T21862 | 113-117 | NN | denotes | goat |
T21863 | 118-128 | NN | denotes | anti-FOXP3 |
T21864 | 129-131 | IN | denotes | in |
T21865 | 132-140 | VB | denotes | blocking |
T21866 | 141-147 | NN | denotes | buffer |
T21867 | 148-149 | -LRB- | denotes | ( |
T21868 | 149-154 | NN | denotes | Abcam |
T21869 | 154-155 | -RRB- | denotes | ) |
T21870 | 156-165 | RB | denotes | overnight |
T21871 | 166-168 | IN | denotes | at |
T21872 | 169-170 | CD | denotes | 4 |
T21873 | 171-174 | NNP | denotes | °C. |
T21874 | 175-178 | DT | denotes | The |
T21875 | 179-184 | NN | denotes | blots |
T21876 | 185-189 | VB | denotes | were |
T21877 | 190-199 | VB | denotes | developed |
T21878 | 200-205 | VB | denotes | using |
T21879 | 206-208 | DT | denotes | an |
T21880 | 209-218 | JJ | denotes | anti-goat |
T21881 | 219-230 | JJ | denotes | HRP-labeled |
T21882 | 231-234 | NN | denotes | mAb |
T21883 | 235-236 | -LRB- | denotes | ( |
T21884 | 236-244 | NNP | denotes | Amersham |
T21885 | 245-256 | NNP | denotes | Biosciences |
T21886 | 256-257 | -RRB- | denotes | ) |
T21887 | 258-261 | CC | denotes | and |
T21888 | 262-272 | VB | denotes | visualized |
T21889 | 273-277 | IN | denotes | with |
T21890 | 278-279 | DT | denotes | a |
T21891 | 280-283 | NN | denotes | LAS |
T21892 | 284-288 | CD | denotes | 1000 |
T21893 | 289-295 | NN | denotes | camera |
T21894 | 296-297 | -LRB- | denotes | ( |
T21895 | 297-301 | NN | denotes | Fuji |
T21896 | 301-302 | -RRB- | denotes | ) |
R16265 | T21843 | T21842 | arg1Of | binding,Unspecific |
R16266 | T21843 | T21844 | arg1Of | binding,was |
R16267 | T21843 | T21845 | arg2Of | binding,blocked |
R16268 | T21845 | T21844 | arg2Of | blocked,was |
R16269 | T21845 | T21846 | arg1Of | blocked,with |
R16270 | T21845 | T21849 | arg1Of | blocked,and |
R16271 | T21847 | T21846 | arg2Of | BSA,with |
R16272 | T21849 | T21848 | arg1Of | and,"," |
R16273 | T21849 | T21858 | arg1Of | and,: |
R16274 | T21851 | T21850 | arg1Of | membranes,the |
R16275 | T21851 | T21852 | arg1Of | membranes,were |
R16276 | T21851 | T21854 | arg2Of | membranes,incubated |
R16277 | T21854 | T21849 | arg2Of | incubated,and |
R16278 | T21854 | T21852 | arg2Of | incubated,were |
R16279 | T21854 | T21853 | arg1Of | incubated,subsequently |
R16280 | T21854 | T21855 | arg1Of | incubated,with |
R16281 | T21857 | T21855 | arg2Of | 1,with |
R16282 | T21857 | T21856 | arg1Of | 1,an |
R16283 | T21860 | T21859 | arg1Of | dilution,200 |
R16284 | T21860 | T21861 | arg1Of | dilution,of |
R16285 | T21860 | T21864 | arg1Of | dilution,in |
R16286 | T21860 | T21876 | arg1Of | dilution,were |
R16287 | T21860 | T21877 | arg2Of | dilution,developed |
R16288 | T21860 | T21888 | arg2Of | dilution,visualized |
R16289 | T21863 | T21861 | arg2Of | anti-FOXP3,of |
R16290 | T21863 | T21862 | arg1Of | anti-FOXP3,goat |
R16291 | T21865 | T21864 | arg2Of | blocking,in |
R16292 | T21865 | T21870 | arg1Of | blocking,overnight |
R16293 | T21865 | T21871 | arg1Of | blocking,at |
R16294 | T21866 | T21865 | arg2Of | buffer,blocking |
R16295 | T21866 | T21867 | arg1Of | buffer,( |
R16296 | T21868 | T21867 | arg2Of | Abcam,( |
R16297 | T21869 | T21867 | arg3Of | ),( |
R16298 | T21873 | T21872 | arg1Of | °C.,4 |
R16299 | T21875 | T21871 | arg2Of | blots,at |
R16300 | T21875 | T21873 | arg1Of | blots,°C. |
R16301 | T21875 | T21874 | arg1Of | blots,The |
R16302 | T21877 | T21887 | arg1Of | developed,and |
R16303 | T21878 | T21877 | arg3Of | using,developed |
R16304 | T21882 | T21878 | arg2Of | mAb,using |
R16305 | T21882 | T21879 | arg1Of | mAb,an |
R16306 | T21882 | T21880 | arg1Of | mAb,anti-goat |
R16307 | T21882 | T21881 | arg1Of | mAb,HRP-labeled |
R16308 | T21882 | T21883 | arg1Of | mAb,( |
R16309 | T21885 | T21883 | arg2Of | Biosciences,( |
R16310 | T21885 | T21884 | arg1Of | Biosciences,Amersham |
R16311 | T21886 | T21883 | arg3Of | ),( |
R16312 | T21887 | T21858 | arg2Of | and,: |
R16313 | T21887 | T21876 | arg2Of | and,were |
R16314 | T21888 | T21887 | arg2Of | visualized,and |
R16315 | T21888 | T21889 | arg1Of | visualized,with |
R16316 | T21893 | T21889 | arg2Of | camera,with |
R16317 | T21893 | T21890 | arg1Of | camera,a |
R16318 | T21893 | T21891 | arg1Of | camera,LAS |
R16319 | T21893 | T21892 | arg1Of | camera,1000 |
R16320 | T21893 | T21894 | arg1Of | camera,( |
R16321 | T21895 | T21894 | arg2Of | Fuji,( |
R16322 | T21896 | T21894 | arg3Of | ),( |
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T22030 | 0-10 | JJ | denotes | Unspecific |
T22031 | 11-18 | JJ | denotes | binding |
T22032 | 19-22 | VBD | denotes | was |
T22033 | 23-30 | VBN | denotes | blocked |
T22034 | 31-35 | IN | denotes | with |
T22035 | 36-39 | NNP | denotes | BSA |
T22036 | 39-40 | , | denotes | , |
T22037 | 41-44 | CC | denotes | and |
T22038 | 45-48 | DT | denotes | the |
T22039 | 49-58 | NNS | denotes | membranes |
T22040 | 59-63 | VBD | denotes | were |
T22041 | 64-76 | RB | denotes | subsequently |
T22042 | 77-86 | VBN | denotes | incubated |
T22043 | 87-91 | IN | denotes | with |
T22044 | 92-94 | DT | denotes | an |
T22045 | 95-100 | CD | denotes | 1:200 |
T22046 | 101-109 | NN | denotes | dilution |
T22047 | 110-112 | IN | denotes | of |
T22048 | 113-117 | NN | denotes | goat |
T22049 | 118-128 | NN | denotes | anti-FOXP3 |
T22050 | 129-131 | IN | denotes | in |
T22051 | 132-140 | VBG | denotes | blocking |
T22052 | 141-147 | NN | denotes | buffer |
T22053 | 148-149 | -LRB- | denotes | ( |
T22054 | 149-154 | NNP | denotes | Abcam |
T22055 | 154-155 | -RRB- | denotes | ) |
T22056 | 156-165 | RB | denotes | overnight |
T22057 | 166-168 | IN | denotes | at |
T22058 | 169-170 | CD | denotes | 4 |
T22059 | 171-172 | CD | denotes | ° |
T22060 | 172-173 | NNP | denotes | C |
T22061 | 173-174 | . | denotes | . |
T22062 | 175-178 | DT | denotes | The |
T22063 | 179-184 | NNS | denotes | blots |
T22064 | 185-189 | VBD | denotes | were |
T22065 | 190-199 | VBN | denotes | developed |
T22066 | 200-205 | VBG | denotes | using |
T22067 | 206-208 | DT | denotes | an |
T22068 | 209-218 | JJ | denotes | anti-goat |
T22069 | 219-230 | JJ | denotes | HRP-labeled |
T22070 | 231-234 | NNP | denotes | mAb |
T22071 | 235-236 | -LRB- | denotes | ( |
T22072 | 236-244 | NNP | denotes | Amersham |
T22073 | 245-256 | NNPS | denotes | Biosciences |
T22074 | 256-257 | -RRB- | denotes | ) |
T22075 | 258-261 | CC | denotes | and |
T22076 | 262-272 | VBN | denotes | visualized |
T22077 | 273-277 | IN | denotes | with |
T22078 | 278-279 | DT | denotes | a |
T22079 | 280-283 | NNP | denotes | LAS |
T22080 | 284-288 | CD | denotes | 1000 |
T22081 | 289-295 | NN | denotes | camera |
T22082 | 296-297 | -LRB- | denotes | ( |
T22083 | 297-301 | NNP | denotes | Fuji |
T22084 | 301-302 | -RRB- | denotes | ) |
T22085 | 302-303 | . | denotes | . |
R16441 | T22030 | T22031 | amod | Unspecific,binding |
R16442 | T22031 | T22033 | nsubjpass | binding,blocked |
R16443 | T22032 | T22033 | auxpass | was,blocked |
R16444 | T22033 | T22033 | ROOT | blocked,blocked |
R16445 | T22034 | T22033 | prep | with,blocked |
R16446 | T22035 | T22034 | pobj | BSA,with |
R16447 | T22036 | T22033 | punct | ",",blocked |
R16448 | T22037 | T22033 | cc | and,blocked |
R16449 | T22038 | T22039 | det | the,membranes |
R16450 | T22039 | T22042 | nsubjpass | membranes,incubated |
R16451 | T22040 | T22042 | auxpass | were,incubated |
R16452 | T22041 | T22042 | advmod | subsequently,incubated |
R16453 | T22042 | T22033 | conj | incubated,blocked |
R16454 | T22043 | T22042 | prep | with,incubated |
R16455 | T22044 | T22046 | det | an,dilution |
R16456 | T22045 | T22046 | nummod | 1:200,dilution |
R16457 | T22046 | T22043 | pobj | dilution,with |
R16458 | T22047 | T22046 | prep | of,dilution |
R16459 | T22048 | T22049 | compound | goat,anti-FOXP3 |
R16460 | T22049 | T22047 | pobj | anti-FOXP3,of |
R16461 | T22050 | T22046 | prep | in,dilution |
R16462 | T22051 | T22050 | pcomp | blocking,in |
R16463 | T22052 | T22051 | dobj | buffer,blocking |
R16464 | T22053 | T22052 | punct | (,buffer |
R16465 | T22054 | T22052 | appos | Abcam,buffer |
R16466 | T22055 | T22052 | punct | ),buffer |
R16467 | T22056 | T22051 | npadvmod | overnight,blocking |
R16468 | T22057 | T22051 | prep | at,blocking |
R16469 | T22058 | T22059 | nummod | 4,° |
R16470 | T22059 | T22057 | pobj | °,at |
R16471 | T22060 | T22050 | pobj | C,in |
R16472 | T22061 | T22042 | punct | .,incubated |
R16473 | T22062 | T22063 | det | The,blots |
R16474 | T22063 | T22065 | nsubjpass | blots,developed |
R16475 | T22064 | T22065 | auxpass | were,developed |
R16476 | T22065 | T22065 | ROOT | developed,developed |
R16477 | T22066 | T22065 | advcl | using,developed |
R16478 | T22067 | T22070 | det | an,mAb |
R16479 | T22068 | T22070 | amod | anti-goat,mAb |
R16480 | T22069 | T22070 | compound | HRP-labeled,mAb |
R16481 | T22070 | T22066 | dobj | mAb,using |
R16482 | T22071 | T22073 | punct | (,Biosciences |
R16483 | T22072 | T22073 | compound | Amersham,Biosciences |
R16484 | T22073 | T22070 | parataxis | Biosciences,mAb |
R16485 | T22074 | T22073 | punct | ),Biosciences |
R16486 | T22075 | T22066 | cc | and,using |
R16487 | T22076 | T22066 | conj | visualized,using |
R16488 | T22077 | T22076 | prep | with,visualized |
R16489 | T22078 | T22081 | det | a,camera |
R16490 | T22079 | T22081 | nmod | LAS,camera |
R16491 | T22080 | T22081 | nummod | 1000,camera |
R16492 | T22081 | T22077 | pobj | camera,with |
R16493 | T22082 | T22081 | punct | (,camera |
R16494 | T22083 | T22081 | appos | Fuji,camera |
R16495 | T22084 | T22081 | punct | ),camera |
R16496 | T22085 | T22065 | punct | .,developed |
GO-MF
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T22157 | 11-18 | http://purl.obolibrary.org/obo/GO_0005488 | denotes | binding |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T22160 | 49-58 | http://purl.obolibrary.org/obo/GO_0016020 | denotes | membranes |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21787 | 0-303 | Sentence | denotes | Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji). |
T326 | 0-174 | Sentence | denotes | Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. |
T327 | 175-303 | Sentence | denotes | The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji). |
events-check-again
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T22204 | 123-128 | Protein | denotes | FOXP3 |
bionlp-st-ge-2016-reference-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T22165 | 113-128 | Protein | denotes | goat anti-FOXP3 |
T22166 | 231-234 | Protein | denotes | mAb |
bionlp-st-ge-2016-reference
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21777 | 123-128 | Protein | denotes | FOXP3 |
bionlp-st-ge-2016-uniprot
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21959 | 123-128 | Q9BZS1 | denotes | FOXP3 |
test2
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21767 | 123-128 | Protein | denotes | FOXP3 |