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2_test

Id Subject Object Predicate Lexical cue
18162042-11441076-84705058 315-317 11441076 denotes 22
18162042-12554698-84705059 812-814 12554698 denotes 60
18162042-16387593-84705060 1405-1407 16387593 denotes 61
18162042-10037236-84705061 2883-2885 10037236 denotes 62
18162042-16517728-84705062 3895-3897 16517728 denotes 24
18162042-17298430-84705063 5347-5349 17298430 denotes 63
T68613 315-317 11441076 denotes 22
T44281 812-814 12554698 denotes 60
T86850 1405-1407 16387593 denotes 61
T11976 2883-2885 10037236 denotes 62
T56825 3895-3897 16517728 denotes 24
T99961 5347-5349 17298430 denotes 63

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T17778 23-27 NN denotes Mice
T17779 29-35 JJ denotes Normal
T17780 36-38 NN denotes B6
T17781 39-43 NN denotes mice
T17782 44-48 VB denotes were
T17783 49-58 VB denotes purchased
T17784 59-63 IN denotes from
T17785 64-67 DT denotes the
T17786 68-75 NNP denotes Jackson
T17787 76-88 NNP denotes Laboratories
T17788 89-90 -LRB- denotes (
T17789 90-93 NNP denotes Bar
T17790 94-100 NNP denotes Harbor
T17791 100-101 -COMMA- denotes ,
T17792 102-107 NNP denotes Maine
T17793 107-108 -RRB- denotes )
T17794 110-120 JJ denotes Transgenic
T17795 121-128 NN denotes DO11.10
T17796 129-133 NN denotes mice
T17797 133-134 -COMMA- denotes ,
T17798 135-145 VB denotes expressing
T17799 146-147 DT denotes a
T17800 148-149 NN denotes T
T17801 150-154 NN denotes cell
T17802 155-163 NN denotes receptor
T17803 164-167 IN denotes for
T17804 168-178 NN denotes OVA323–339
T17805 179-186 NN denotes peptide
T17806 187-189 IN denotes in
T17807 190-193 DT denotes the
T17808 194-201 NN denotes context
T17809 202-204 IN denotes of
T17810 205-209 NN denotes H-2d
T17811 209-210 -COMMA- denotes ,
T17812 211-215 VB denotes were
T17813 216-227 VB denotes backcrossed
T17814 228-232 IN denotes with
T17815 233-237 NN denotes mice
T17816 238-248 VB denotes expressing
T17817 249-255 NN denotes GATA-3
T17818 255-256 -COMMA- denotes ,
T17819 257-263 VB denotes driven
T17820 264-266 IN denotes by
T17821 267-270 DT denotes the
T17822 271-276 JJ denotes human
T17823 277-280 NN denotes CD2
T17824 281-286 NN denotes locus
T17825 287-294 NN denotes control
T17826 295-301 NN denotes region
T17827 302-303 -LRB- denotes (
T17828 303-312 NN denotes CD2-GATA3
T17829 312-313 -RRB- denotes )
T17830 314-315 -LRB- denotes [
T17831 315-317 CD denotes 22
T17832 317-318 -RRB- denotes ]
T17833 318-319 -COMMA- denotes ,
T17834 320-329 VB denotes resulting
T17835 330-332 IN denotes in
T17836 333-350 NN denotes DO11.10xCD2-GATA3
T17837 351-355 NN denotes mice
T17838 357-361 NN denotes Mice
T17839 362-366 VB denotes used
T17840 367-370 IN denotes for
T17841 371-382 NN denotes experiments
T17842 383-387 VB denotes were
T17843 388-399 VB denotes backcrossed
T17844 400-402 IN denotes on
T17845 403-404 DT denotes a
T17846 405-411 NN denotes BALB/c
T17847 412-422 NN denotes background
T17848 423-426 IN denotes for
T17849 427-428 DT denotes a
T17850 429-436 NN denotes minimum
T17851 437-439 IN denotes of
T17852 440-445 CD denotes eight
T17853 446-457 NN denotes generations
T17854 458-461 CC denotes and
T17855 462-466 VB denotes used
T17856 467-469 IN denotes at
T17857 470-472 DT denotes an
T17858 473-476 NN denotes age
T17859 477-479 IN denotes of
T17860 480-484 CD denotes 8–12
T17861 485-487 NN denotes wk
T17862 489-493 NN denotes Mice
T17863 494-498 VB denotes were
T17864 499-505 VB denotes housed
T17865 506-511 IN denotes under
T17866 512-520 JJ denotes specific
T17867 521-534 JJ denotes pathogen-free
T17868 535-545 NN denotes conditions
T17869 546-549 CC denotes and
T17870 550-553 DT denotes all
T17871 554-560 NN denotes animal
T17872 561-568 NN denotes studies
T17873 569-573 VB denotes were
T17874 574-583 VB denotes performed
T17875 584-593 VB denotes according
T17876 594-596 TO denotes to
T17877 597-610 JJ denotes institutional
T17878 611-614 CC denotes and
T17879 615-620 NN denotes state
T17880 621-631 NN denotes guidelines
T18081 634-643 NN denotes Isolation
T18082 644-646 IN denotes of
T18083 647-651 JJ denotes CD4+
T18084 652-653 NN denotes T
T18085 654-659 NN denotes cells
T18086 661-665 JJ denotes CD4+
T18087 666-667 NN denotes T
T18088 668-673 NN denotes cells
T18089 674-678 VB denotes were
T18090 679-687 VB denotes isolated
T18091 688-692 IN denotes from
T18092 693-698 NN denotes blood
T18093 699-701 IN denotes of
T18094 702-709 JJ denotes healthy
T18095 710-715 JJ denotes human
T18096 716-726 NN denotes volunteers
T18097 727-732 VB denotes using
T18098 733-736 DT denotes the
T18099 737-745 JJ denotes anti-CD4
T18100 746-754 JJ denotes magnetic
T18101 755-760 NN denotes beads
T18102 761-762 -LRB- denotes (
T18103 762-767 NNP denotes Dynal
T18104 767-768 -COMMA- denotes ,
T18105 769-776 NNP denotes Hamburg
T18106 776-777 -COMMA- denotes ,
T18107 778-785 NNP denotes Germany
T18108 785-786 -RRB- denotes )
T18109 787-789 IN denotes as
T18110 790-800 RB denotes previously
T18111 801-810 VB denotes described
T18112 811-812 -LRB- denotes [
T18113 812-814 CD denotes 60
T18114 814-815 -RRB- denotes ]
T18115 817-820 DT denotes The
T18116 821-827 NN denotes purity
T18117 828-830 IN denotes of
T18118 831-835 JJ denotes CD4+
T18119 836-837 NN denotes T
T18120 838-843 NN denotes cells
T18121 844-847 VB denotes was
T18122 848-857 RB denotes initially
T18123 858-864 VB denotes tested
T18124 865-867 IN denotes by
T18125 868-872 NN denotes FACS
T18126 873-876 CC denotes and
T18127 877-880 VB denotes was
T18128 881-882 RB denotes ≥
T18129 883-885 CD denotes 95
T18130 885-886 NN denotes %
T18131 888-901 NN denotes Monoclonality
T18132 902-904 IN denotes of
T18133 905-906 NN denotes T
T18134 907-911 NN denotes cell
T18135 912-918 NN denotes clones
T18136 919-922 VB denotes was
T18137 923-932 VB denotes confirmed
T18138 933-935 IN denotes by
T18139 936-945 JJ denotes TCR-chain
T18140 946-953 NN denotes mapping
T18141 954-957 CC denotes and
T18142 958-961 VB denotes was
T18143 962-972 VB denotes identified
T18144 973-975 TO denotes to
T18145 976-978 VB denotes be
T18146 979-985 NN denotes Vbeta8
T18147 986-994 JJ denotes positive
T18148 996-999 DT denotes The
T18149 1000-1006 NN denotes clones
T18150 1007-1011 VB denotes were
T18151 1012-1025 VB denotes characterized
T18152 1026-1028 IN denotes by
T18153 1029-1033 JJ denotes high
T18154 1034-1038 NN denotes IL-4
T18155 1039-1048 NN denotes secretion
T18793 2635-2644 JJ denotes Inducible
T18794 2645-2651 JJ denotes murine
T18795 2652-2656 NN denotes Treg
T18796 2657-2664 NN denotes culture
T18797 2666-2671 JJ denotes Naive
T18798 2672-2676 JJ denotes CD4+
T18799 2677-2678 NN denotes T
T18800 2679-2684 NN denotes cells
T18801 2685-2686 -LRB- denotes (
T18802 2686-2690 JJ denotes CD4+
T18803 2690-2691 -COMMA- denotes ,
T18804 2692-2698 JJ denotes CD62L+
T18805 2698-2699 -COMMA- denotes ,
T18806 2700-2703 CC denotes and
T18807 2704-2709 NN denotes CD25–
T18808 2709-2710 -RRB- denotes )
T18809 2711-2715 VB denotes were
T18810 2716-2724 VB denotes isolated
T18811 2725-2729 IN denotes from
T18812 2730-2736 VB denotes pooled
T18813 2737-2742 NN denotes lymph
T18814 2743-2748 NN denotes nodes
T18815 2749-2752 CC denotes and
T18816 2753-2760 NN denotes spleens
T18817 2761-2763 IN denotes by
T18818 2764-2768 NN denotes FACS
T18819 2769-2770 -LRB- denotes (
T18820 2770-2774 NNP denotes FACS
T18821 2775-2779 NNP denotes Aria
T18822 2779-2780 -COMMA- denotes ,
T18823 2781-2783 NNP denotes BD
T18824 2784-2795 NNP denotes Biosciences
T18825 2795-2796 -RRB- denotes )
T18826 2798-2799 CD denotes 5
T18827 2800-2801 IN denotes ×
T18828 2802-2805 CD denotes 105
T18829 2806-2807 NN denotes T
T18830 2808-2813 NN denotes cells
T18831 2814-2818 VB denotes were
T18832 2819-2830 VB denotes co-cultured
T18833 2831-2835 IN denotes with
T18834 2836-2839 CD denotes 2.5
T18835 2840-2841 -COLON- denotes ×
T18836 2842-2845 CD denotes 104
T18837 2846-2850 NN denotes bone
T18838 2851-2865 VB denotes marrow–derived
T18839 2866-2875 JJ denotes dendritic
T18840 2876-2881 NN denotes cells
T18841 2882-2883 -LRB- denotes [
T18842 2883-2885 CD denotes 62
T18843 2885-2886 -RRB- denotes ]
T18844 2887-2890 CC denotes and
T18845 2891-2895 CD denotes 0.01
T18846 2896-2901 NN denotes μg/ml
T18847 2902-2912 NN denotes OVA323–339
T18848 2913-2920 NN denotes peptide
T18849 2921-2922 -LRB- denotes (
T18850 2922-2929 NN denotes Ansynth
T18851 2929-2930 -RRB- denotes )
T18852 2931-2933 IN denotes in
T18853 2934-2937 DT denotes the
T18854 2938-2946 NN denotes presence
T18855 2947-2949 CC denotes or
T18856 2950-2957 NN denotes absence
T18857 2958-2960 IN denotes of
T18858 2961-2969 JJ denotes 20-ng/μl
T18859 2970-2978 NN denotes rhTGF-ß1
T18860 2979-2980 -LRB- denotes (
T18861 2980-2989 NN denotes Peprotech
T18862 2989-2990 -RRB- denotes )
T18863 2991-2993 IN denotes in
T18864 2994-3001 JJ denotes 48-well
T18865 3002-3008 NN denotes plates
T18866 3010-3015 IN denotes After
T18867 3016-3017 CD denotes 4
T18868 3018-3019 NN denotes d
T18869 3019-3020 -COMMA- denotes ,
T18870 3021-3026 NN denotes cells
T18871 3027-3031 VB denotes were
T18872 3032-3041 VB denotes harvested
T18873 3042-3045 CC denotes and
T18874 3046-3054 VB denotes analyzed
T18875 3055-3058 IN denotes for
T18876 3059-3072 JJ denotes intracellular
T18877 3073-3078 NN denotes FOXP3
T18878 3079-3089 NN denotes expression
T18879 3090-3092 IN denotes by
T18880 3093-3097 NN denotes FACS
T18881 3098-3100 CC denotes or
T18882 3101-3105 NN denotes gene
T18883 3106-3116 NN denotes expression
T18884 3117-3119 IN denotes by
T18885 3120-3132 JJ denotes quantitative
T18886 3133-3139 NN denotes RT-PCR
T19113 3142-3144 FW denotes In
T19114 3145-3150 FW denotes vitro
T19115 3151-3152 NN denotes T
T19116 3153-3157 NN denotes cell
T19117 3158-3173 NN denotes differentiation
T19118 3175-3179 JJ denotes CD4+
T19119 3180-3187 JJ denotes CD45RA+
T19120 3188-3207 JJ denotes magnetically-sorted
T19121 3208-3209 -LRB- denotes (
T19122 3209-3215 NN denotes CD45RO
T19123 3216-3225 NN denotes depletion
T19124 3225-3226 -COMMA- denotes ,
T19125 3227-3231 NN denotes MACS
T19126 3231-3232 -COMMA- denotes ,
T19127 3233-3242 VB denotes according
T19128 3243-3245 TO denotes to
T19129 3246-3249 DT denotes the
T19130 3250-3258 NN denotes protocol
T19131 3259-3261 IN denotes of
T19132 3262-3265 DT denotes the
T19133 3266-3278 NN denotes manufacturer
T19134 3278-3279 -RRB- denotes )
T19135 3280-3285 NN denotes cells
T19136 3286-3290 VB denotes were
T19137 3291-3301 VB denotes stimulated
T19138 3302-3306 IN denotes with
T19139 3307-3318 VB denotes immobilized
T19140 3319-3330 JJ denotes plate-bound
T19141 3331-3339 NN denotes anti-CD3
T19142 3340-3341 -LRB- denotes (
T19143 3341-3342 CD denotes 1
T19144 3343-3348 NN denotes μg/ml
T19145 3348-3349 -COMMA- denotes ,
T19146 3350-3354 NN denotes Okt3
T19147 3354-3355 -COMMA- denotes ,
T19148 3356-3360 NN denotes IgG1
T19149 3360-3361 -RRB- denotes )
T19150 3362-3365 CC denotes and
T19151 3366-3375 JJ denotes anti-CD28
T19152 3376-3377 -LRB- denotes (
T19153 3377-3378 CD denotes 2
T19154 3379-3384 NN denotes μg/ml
T19155 3384-3385 -RRB- denotes )
T19156 3386-3388 IN denotes in
T19157 3389-3392 NN denotes Th1
T19158 3393-3403 NN denotes conditions
T19159 3403-3404 -COLON- denotes :
T19160 3405-3407 CD denotes 25
T19161 3408-3413 NN denotes ng/ml
T19162 3414-3419 NN denotes IL-12
T19163 3419-3420 -COMMA- denotes ,
T19164 3421-3422 CD denotes 5
T19165 3423-3428 NN denotes μg/ml
T19166 3429-3438 NN denotes anti-IL-4
T19167 3439-3440 -LRB- denotes (
T19168 3440-3441 NN denotes R
T19169 3441-3442 CC denotes &
T19170 3442-3443 NN denotes D
T19171 3444-3451 NN denotes systems
T19172 3451-3452 -RRB- denotes )
T19173 3452-3453 -COLON- denotes ;
T19174 3454-3456 IN denotes in
T19175 3457-3460 NN denotes Th2
T19176 3461-3471 NN denotes conditions
T19177 3471-3472 -COLON- denotes :
T19178 3473-3475 CD denotes 25
T19179 3476-3481 NN denotes ng/ml
T19180 3482-3486 NN denotes IL-4
T19181 3486-3487 -COMMA- denotes ,
T19182 3488-3489 CD denotes 5
T19183 3490-3495 NN denotes μg/ml
T19184 3496-3506 JJ denotes anti-IFN-γ
T19185 3506-3507 -COMMA- denotes ,
T19186 3508-3509 CD denotes 5
T19187 3510-3515 NN denotes μg/ml
T19188 3516-3526 NN denotes anti-IL-12
T19189 3527-3528 -LRB- denotes (
T19190 3528-3529 NN denotes R
T19191 3529-3530 CC denotes &
T19192 3530-3531 NN denotes D
T19193 3532-3539 NN denotes systems
T19194 3539-3540 -RRB- denotes )
T19195 3540-3541 -COLON- denotes ;
T19196 3542-3544 CC denotes or
T19197 3545-3547 IN denotes in
T19198 3548-3552 NNP denotes Treg
T19199 3553-3563 NN denotes conditions
T19200 3563-3564 -COLON- denotes :
T19201 3565-3567 CD denotes 10
T19202 3568-3573 NN denotes ng/ml
T19203 3574-3579 NN denotes TGF-β
T19204 3579-3580 -COMMA- denotes ,
T19205 3581-3582 CD denotes 5
T19206 3583-3588 NN denotes μg/ml
T19207 3589-3599 JJ denotes anti-IFN-γ
T19208 3599-3600 -COMMA- denotes ,
T19209 3601-3602 CD denotes 5
T19210 3603-3608 NN denotes μg/ml
T19211 3609-3619 NN denotes anti-IL-12
T19212 3619-3620 -COMMA- denotes ,
T19213 3621-3622 CD denotes 5
T19214 3623-3628 NN denotes μg/ml
T19215 3629-3638 NN denotes anti-IL-4
T19216 3640-3653 VB denotes Proliferating
T19217 3654-3659 NN denotes cells
T19218 3660-3664 VB denotes were
T19219 3665-3673 VB denotes expanded
T19220 3674-3676 IN denotes in
T19221 3677-3683 NN denotes medium
T19222 3684-3694 VB denotes containing
T19223 3695-3699 NN denotes IL-2
T19224 3700-3701 -LRB- denotes (
T19225 3701-3703 CD denotes 30
T19226 3704-3709 NN denotes ng/ml
T19227 3709-3710 -RRB- denotes )
T19537 3812-3816 IN denotes into
T19538 3817-3820 DT denotes the
T19539 3821-3825 NN denotes pGL3
T19540 3826-3831 JJ denotes basic
T19541 3832-3838 NN denotes vector
T19542 3839-3840 -LRB- denotes (
T19543 3840-3847 NNP denotes Promega
T19544 3848-3855 NNP denotes Biotech
T19545 3855-3856 -RRB- denotes )
T19546 3857-3859 TO denotes to
T19547 3860-3868 VB denotes generate
T19548 3869-3872 DT denotes the
T19549 3873-3877 NN denotes pGL3
T19550 3878-3883 NN denotes FOXP3
T19551 3884-3893 CD denotes −511/+176
T19552 3894-3895 -LRB- denotes [
T19553 3895-3897 CD denotes 24
T19554 3897-3898 -RRB- denotes ]
T19555 3900-3913 JJ denotes Site-directed
T19556 3914-3925 NN denotes mutagenesis
T19557 3926-3928 IN denotes in
T19558 3929-3932 DT denotes the
T19559 3933-3938 NN denotes FOXP3
T19560 3939-3947 NN denotes promoter
T19561 3948-3954 NN denotes region
T19562 3955-3959 VB denotes were
T19563 3960-3970 VB denotes introduced
T19564 3971-3976 VB denotes using
T19565 3977-3980 DT denotes the
T19566 3981-3992 NN denotes QuickChange
T19567 3993-3996 NN denotes kit
T19568 3997-3998 -LRB- denotes (
T19569 3998-4008 NN denotes Stratagene
T19570 4008-4009 -RRB- denotes )
T19571 4009-4010 -COMMA- denotes ,
T19572 4011-4020 VB denotes according
T19573 4021-4023 TO denotes to
T19574 4024-4027 DT denotes the
T19575 4028-4040 NN denotes manufacturer
T19576 4040-4042 POS denotes 's
T19577 4043-4055 NN denotes instructions
T19578 4057-4060 DT denotes The
T19579 4061-4070 VB denotes following
T19580 4071-4077 NN denotes primer
T19581 4078-4081 CC denotes and
T19582 4082-4085 PRP-DOLLAR- denotes its
T19583 4086-4099 JJ denotes complementary
T19584 4100-4106 NN denotes strand
T19585 4107-4111 VB denotes were
T19586 4112-4116 VB denotes used
T19587 4116-4117 -COLON- denotes :
T19588 4118-4121 NN denotes GTT
T19589 4122-4125 NN denotes TCT
T19590 4126-4129 NN denotes CAT
T19591 4130-4133 NNP denotes GAG
T19592 4134-4137 NNP denotes CCC
T19593 4138-4141 NN denotes TAT
T19594 4142-4145 NNP denotes TAA
T19595 4146-4149 NNP denotes GTC
T19596 4150-4153 NNP denotes ATT
T19597 4154-4157 NNP denotes CTT
T19598 4158-4161 NNP denotes ACC
T19599 4162-4165 NN denotes TCT
T19600 4166-4169 NNP denotes CAC
T19601 4170-4173 NNP denotes CTC
T19602 4174-4177 NNP denotes TGT
T19603 4178-4181 NNP denotes GGT
T19604 4182-4184 NNP denotes GA
T19770 4187-4200 NN denotes Transfections
T19771 4201-4204 CC denotes and
T19772 4205-4213 NN denotes reporter
T19773 4214-4218 NN denotes gene
T19774 4219-4225 NN denotes assays
T19775 4227-4228 NN denotes T
T19776 4229-4234 NN denotes cells
T19777 4235-4239 VB denotes were
T19778 4240-4246 VB denotes rested
T19779 4247-4249 IN denotes in
T19780 4250-4260 JJ denotes serum-free
T19781 4261-4266 NN denotes AIM-V
T19782 4267-4273 NN denotes medium
T19783 4274-4275 -LRB- denotes (
T19784 4275-4279 NNP denotes Life
T19785 4280-4292 NNP denotes Technologies
T19786 4292-4293 -RRB- denotes )
T19787 4294-4303 RB denotes overnight
T19788 4305-4308 CD denotes 3.5
T19789 4309-4311 NN denotes μg
T19790 4312-4314 IN denotes of
T19791 4315-4318 DT denotes the
T19792 4319-4324 NN denotes FOXP3
T19793 4325-4333 NN denotes promoter
T19794 4334-4344 NN denotes luciferase
T19795 4345-4353 NN denotes reporter
T19796 4354-4360 NN denotes vector
T19797 4361-4364 CC denotes and
T19798 4365-4368 CD denotes 0.5
T19799 4369-4371 NN denotes μg
T19800 4372-4379 NN denotes phRL-TK
T19801 4380-4384 VB denotes were
T19802 4385-4390 VB denotes added
T19803 4391-4393 TO denotes to
T19804 4394-4395 CD denotes 3
T19805 4396-4397 SYM denotes ×
T19806 4398-4401 CD denotes 106
T19807 4402-4406 JJ denotes CD4+
T19808 4407-4408 NN denotes T
T19809 4409-4414 NN denotes cells
T19810 4415-4426 VB denotes resuspended
T19811 4427-4429 IN denotes in
T19812 4430-4433 CD denotes 100
T19813 4434-4436 NN denotes μL
T19814 4437-4439 IN denotes of
T19815 4440-4452 NN denotes Nucleofector
T19816 4453-4461 NN denotes solution
T19817 4462-4463 -LRB- denotes (
T19818 4463-4468 NNP denotes Amaxa
T19819 4469-4479 NNP denotes Biosystems
T19820 4479-4480 -RRB- denotes )
T19821 4481-4484 CC denotes and
T19822 4485-4499 VB denotes electroporated
T19823 4500-4505 VB denotes using
T19824 4506-4509 DT denotes the
T19825 4510-4514 NN denotes U-15
T19826 4515-4522 NN denotes program
T19827 4523-4525 IN denotes of
T19828 4526-4529 DT denotes the
T19829 4530-4543 NNP denotes Nucleofector.
T19830 4544-4549 IN denotes After
T19831 4550-4551 DT denotes a
T19832 4552-4556 JJ denotes 24-h
T19833 4557-4564 NN denotes culture
T19834 4565-4567 IN denotes in
T19835 4568-4578 JJ denotes serum-free
T19836 4579-4589 NN denotes conditions
T19837 4590-4593 CC denotes and
T19838 4594-4601 NN denotes stimuli
T19839 4602-4604 IN denotes as
T19840 4605-4614 VB denotes indicated
T19841 4615-4617 IN denotes in
T19842 4618-4621 DT denotes the
T19843 4622-4629 NN denotes figures
T19844 4629-4630 -COMMA- denotes ,
T19845 4631-4641 NN denotes luciferase
T19846 4642-4650 NN denotes activity
T19847 4651-4654 VB denotes was
T19848 4655-4663 VB denotes measured
T19849 4664-4666 IN denotes by
T19850 4667-4670 DT denotes the
T19851 4671-4675 JJ denotes dual
T19852 4676-4686 NN denotes luciferase
T19853 4687-4692 NN denotes assay
T19854 4693-4699 NN denotes system
T19855 4700-4701 -LRB- denotes (
T19856 4701-4708 NNP denotes Promega
T19857 4709-4716 NNP denotes Biotech
T19858 4716-4717 -RRB- denotes )
T19859 4718-4727 VB denotes according
T19860 4728-4730 TO denotes to
T19861 4731-4734 DT denotes the
T19862 4735-4747 NN denotes manufacturer
T19863 4747-4749 POS denotes 's
T19864 4750-4762 NN denotes instructions
T19865 4764-4768 NN denotes Data
T19866 4769-4773 VB denotes were
T19867 4774-4784 VB denotes normalized
T19868 4785-4787 IN denotes by
T19869 4788-4791 DT denotes the
T19870 4792-4800 NN denotes activity
T19871 4801-4803 IN denotes of
T19872 4804-4811 NN denotes renilla
T19873 4812-4822 NN denotes luciferase
T20080 5006-5010 IN denotes with
T20081 5011-5017 NNP denotes TaqMan
T20082 5018-5039 NN denotes reverse-transcription
T20083 5040-5048 NN denotes reagents
T20084 5049-5050 -LRB- denotes (
T20085 5050-5057 NNP denotes Applied
T20086 5058-5068 NNP denotes Biosystems
T20087 5068-5069 -RRB- denotes )
T20088 5070-5074 IN denotes with
T20089 5075-5081 JJ denotes random
T20090 5082-5090 NN denotes hexamers
T20091 5091-5100 VB denotes according
T20092 5101-5103 TO denotes to
T20093 5104-5107 DT denotes the
T20094 5108-5120 NN denotes manufacturer
T20095 5120-5122 POS denotes 's
T20096 5123-5131 NN denotes protocol
T20175 5134-5145 JJ denotes Recombinant
T20176 5146-5149 NN denotes TAT
T20177 5150-5158 NN denotes proteins
T20178 5160-5163 DT denotes The
T20179 5164-5169 NN denotes cDNAs
T20180 5170-5178 VB denotes encoding
T20181 5179-5184 NN denotes GATA3
T20182 5185-5192 NN denotes protein
T20183 5193-5195 CC denotes or
T20184 5196-5199 DT denotes the
T20185 5200-5209 VB denotes truncated
T20186 5210-5215 NN denotes GATA3
T20187 5216-5217 -LRB- denotes (
T20188 5217-5224 VB denotes lacking
T20189 5225-5228 DT denotes the
T20190 5229-5232 CD denotes two
T20191 5233-5237 NN denotes zinc
T20192 5238-5245 NN denotes fingers
T20193 5245-5246 -RRB- denotes )
T20194 5247-5251 VB denotes were
T20195 5252-5258 VB denotes cloned
T20196 5259-5261 IN denotes in
T20197 5262-5267 NN denotes frame
T20198 5268-5272 IN denotes into
T20199 5273-5275 DT denotes an
T20200 5276-5286 NN denotes expression
T20201 5287-5293 NN denotes vector
T20202 5294-5299 IN denotes along
T20203 5300-5304 IN denotes with
T20204 5305-5308 DT denotes the
T20205 5309-5312 NN denotes TAT
T20206 5313-5321 NN denotes sequence
T20207 5322-5324 IN denotes as
T20208 5325-5335 RB denotes previously
T20209 5336-5345 VB denotes described
T20210 5346-5347 -LRB- denotes [
T20211 5347-5349 CD denotes 63
T20212 5349-5350 -RRB- denotes ]
T20213 5352-5360 NN denotes Proteins
T20214 5361-5365 VB denotes were
T20215 5366-5375 VB denotes expressed
T20216 5376-5378 IN denotes in
T20217 5379-5383 NN denotes BL21
T20218 5384-5388 NN denotes Star
T20219 5389-5390 -LRB- denotes (
T20220 5390-5393 NN denotes DE3
T20221 5393-5394 -RRB- denotes )
T20222 5394-5399 NN denotes pLysS
T20223 5400-5401 -LRB- denotes (
T20224 5401-5411 NN denotes Invitrogen
T20225 5411-5412 -RRB- denotes )
T20226 5413-5416 CC denotes and
T20227 5417-5424 NN denotes lysates
T20228 5425-5429 VB denotes were
T20229 5430-5438 VB denotes purified
T20230 5439-5441 IN denotes by
T20231 5442-5454 JJ denotes Ni2+-chelate
T20232 5455-5461 NN denotes column
T20233 5462-5476 NN denotes chromatography
T20234 5478-5482 DT denotes Both
T20235 5483-5493 JJ denotes TAT-linked
T20236 5494-5502 NN denotes proteins
T20237 5503-5507 VB denotes were
T20238 5508-5512 JJ denotes more
T20239 5513-5517 IN denotes than
T20240 5518-5520 CD denotes 95
T20241 5520-5521 NN denotes %
T20242 5522-5526 JJ denotes pure
T20243 5526-5527 -COMMA- denotes ,
T20244 5528-5533 VB denotes based
T20245 5534-5536 IN denotes on
T20246 5537-5546 NNP denotes Coomassie
T20247 5547-5551 JJ denotes blue
T20248 5552-5560 NN denotes staining
T20249 5561-5563 IN denotes of
T20250 5564-5570 NN denotes sodium
T20251 5571-5580 NN denotes disulfate
T20252 5581-5591 NN denotes acrylamide
T20253 5592-5596 NN denotes gels
T20445 5599-5608 NN denotes TAT-GATA3
T20446 5609-5621 NN denotes transduction
T20447 5623-5634 JJ denotes CD4+CD45RA+
T20448 5635-5640 NN denotes cells
T20449 5641-5645 VB denotes were
T20450 5646-5654 VB denotes cultured
T20451 5655-5657 IN denotes in
T20452 5658-5662 NN denotes AIMV
T20453 5663-5669 NN denotes medium
T20454 5670-5673 CC denotes and
T20455 5674-5684 VB denotes transduced
T20456 5685-5689 IN denotes with
T20457 5690-5692 CD denotes 20
T20458 5693-5695 NN denotes nM
T20459 5695-5696 -COMMA- denotes ,
T20460 5697-5699 CD denotes 10
T20461 5700-5702 NN denotes nM
T20462 5702-5703 -COMMA- denotes ,
T20463 5704-5706 CC denotes or
T20464 5707-5710 CD denotes 500
T20465 5711-5713 NN denotes nM
T20466 5714-5716 IN denotes of
T20467 5717-5728 JJ denotes full-length
T20468 5729-5731 CC denotes or
T20469 5732-5741 VB denotes truncated
T20470 5742-5747 NN denotes GATA3
T20471 5748-5752 IN denotes over
T20472 5753-5756 DT denotes the
T20473 5757-5763 NN denotes course
T20474 5764-5766 IN denotes of
T20475 5767-5768 CD denotes 4
T20476 5769-5770 NN denotes h
T20477 5772-5777 IN denotes After
T20478 5778-5779 CD denotes 4
T20479 5780-5781 NN denotes h
T20480 5781-5782 -COMMA- denotes ,
T20481 5783-5786 DT denotes the
T20482 5787-5792 NN denotes cells
T20483 5793-5797 VB denotes were
T20484 5798-5804 VB denotes washed
T20485 5805-5808 CC denotes and
T20486 5809-5818 VB denotes activated
T20487 5819-5823 IN denotes with
T20488 5824-5831 JJ denotes soluble
T20489 5832-5840 NN denotes anti-CD3
T20490 5841-5844 CC denotes and
T20491 5845-5854 NN denotes anti-CD28
T20492 5855-5858 CC denotes and
T20493 5859-5864 NN denotes TGF-β
T20494 5865-5866 -LRB- denotes (
T20495 5866-5868 CD denotes 10
T20496 5869-5874 NN denotes ng/ml
T20497 5874-5875 -RRB- denotes )
T20498 5877-5881 DT denotes Each
T20499 5882-5885 NN denotes day
T20500 5885-5886 -COMMA- denotes ,
T20501 5887-5890 DT denotes the
T20502 5891-5894 NN denotes TAT
T20503 5895-5903 NN denotes proteins
T20504 5904-5908 VB denotes were
T20505 5909-5916 RB denotes freshly
T20506 5917-5922 VB denotes added
T20507 5923-5925 TO denotes to
T20508 5926-5929 DT denotes the
T20509 5930-5936 NN denotes medium
T20510 5938-5943 NN denotes FOXP3
T20511 5944-5954 NN denotes expression
T20512 5955-5958 VB denotes was
T20513 5959-5967 VB denotes measured
T20514 5968-5973 IN denotes after
T20515 5974-5975 CD denotes 5
T20516 5976-5977 NN denotes d
T20517 5978-5980 IN denotes by
T20518 5981-5994 JJ denotes intracellular
T20519 5995-6003 NN denotes staining
T20715 6006-6019 JJ denotes Intracellular
T20716 6020-6028 NN denotes cytokine
T20717 6029-6037 NN denotes staining
T20718 6039-6040 NN denotes T
T20719 6041-6046 NN denotes cells
T20720 6047-6051 VB denotes were
T20721 6052-6062 VB denotes stimulated
T20722 6063-6067 IN denotes with
T20723 6068-6069 CD denotes 2
T20724 6070-6071 SYM denotes ×
T20725 6072-6076 CD denotes 10−7
T20726 6077-6078 NN denotes M
T20727 6079-6082 NN denotes PMA
T20728 6083-6086 CC denotes and
T20729 6087-6088 CD denotes 1
T20730 6089-6094 NN denotes μg/ml
T20731 6095-6097 IN denotes of
T20732 6098-6107 NN denotes ionomycin
T20733 6108-6109 -LRB- denotes (
T20734 6109-6114 NNP denotes Sigma
T20735 6115-6124 NNP denotes Chemicals
T20736 6124-6125 -RRB- denotes )
T20737 6126-6129 IN denotes for
T20738 6130-6131 CD denotes 4
T20739 6132-6133 NN denotes h
T20740 6135-6138 DT denotes The
T20741 6139-6148 VB denotes following
T20742 6149-6152 NN denotes mAb
T20743 6153-6156 VB denotes was
T20744 6157-6161 VB denotes used
T20745 6161-6162 -COLON- denotes :
T20746 6163-6175 JJ denotes anti-IL-4-PE
T20747 6176-6177 -LRB- denotes (
T20748 6177-6182 NN denotes 8D4–8
T20749 6182-6183 -COMMA- denotes ,
T20750 6184-6186 NN denotes BD
T20751 6186-6187 -RRB- denotes )
T20752 6189-6196 VB denotes Matched
T20753 6197-6204 NN denotes isotype
T20754 6205-6213 NN denotes controls
T20755 6214-6218 VB denotes were
T20756 6219-6223 VB denotes used
T20757 6224-6226 IN denotes at
T20758 6227-6230 DT denotes the
T20759 6231-6235 JJ denotes same
T20760 6236-6243 NN denotes protein
T20761 6244-6257 NN denotes concentration
T20762 6258-6260 IN denotes as
T20763 6261-6264 DT denotes the
T20764 6265-6275 JJ denotes respective
T20765 6276-6286 NN denotes antibodies
T20766 6288-6298 JJ denotes Four-color
T20767 6299-6303 NN denotes FACS
T20768 6304-6307 VB denotes was
T20769 6308-6317 VB denotes performed
T20770 6318-6323 VB denotes using
T20771 6324-6326 DT denotes an
T20772 6327-6332 NN denotes EPICS
T20773 6333-6339 NN denotes XL-MCL
T20774 6340-6341 -LRB- denotes (
T20775 6341-6348 NNP denotes Beckman
T20776 6349-6356 NNP denotes Coulter
T20777 6356-6357 -RRB- denotes )
T20778 6358-6363 VB denotes using
T20779 6364-6367 DT denotes the
T20780 6368-6376 NN denotes software
T20781 6377-6383 NN denotes Expo32
T20782 6384-6391 NN denotes version
T20783 6392-6395 IN denotes for
T20784 6396-6400 NN denotes data
T20785 6401-6412 NN denotes acquisition
T20786 6413-6416 CC denotes and
T20787 6417-6427 NN denotes evaluation
T20968 6430-6434 NN denotes Flow
T20969 6435-6444 NN denotes cytometry
T20970 6446-6449 IN denotes For
T20971 6450-6458 NN denotes analysis
T20972 6459-6461 IN denotes of
T20973 6462-6467 NN denotes FOXP3
T20974 6468-6478 NN denotes expression
T20975 6479-6481 IN denotes at
T20976 6482-6485 DT denotes the
T20977 6486-6497 JJ denotes single-cell
T20978 6498-6503 NN denotes level
T20979 6503-6504 -COMMA- denotes ,
T20980 6505-6510 NN denotes cells
T20981 6511-6515 VB denotes were
T20982 6516-6521 RB denotes first
T20983 6522-6529 VB denotes stained
T20984 6530-6534 IN denotes with
T20985 6535-6538 DT denotes the
T20986 6539-6549 JJ denotes monoclonal
T20987 6550-6558 NN denotes antibody
T20988 6559-6563 NN denotes CD25
T20989 6564-6565 -LRB- denotes (
T20990 6565-6572 NNP denotes Beckman
T20991 6573-6580 NNP denotes Coulter
T20992 6580-6581 -RRB- denotes )
T20993 6581-6582 -COMMA- denotes ,
T20994 6583-6586 CC denotes and
T20995 6587-6592 IN denotes after
T20996 6593-6601 NN denotes fixation
T20997 6602-6605 CC denotes and
T20998 6606-6622 NN denotes permeabilization
T20999 6622-6623 -COMMA- denotes ,
T21000 6624-6629 NN denotes cells
T21001 6630-6634 VB denotes were
T21002 6635-6644 VB denotes incubated
T21003 6645-6649 IN denotes with
T21004 6650-6663 JJ denotes PE-conjugated
T21005 6664-6674 JJ denotes monoclonal
T21006 6675-6683 NN denotes antibody
T21007 6684-6690 NN denotes PCH101
T21008 6691-6692 -LRB- denotes (
T21009 6692-6702 JJ denotes anti-human
T21010 6703-6708 NN denotes FOXP3
T21011 6708-6709 -COLON- denotes ;
T21012 6710-6721 NN denotes eBioscience
T21013 6721-6722 -RRB- denotes )
T21014 6723-6728 VB denotes based
T21015 6729-6731 IN denotes on
T21016 6732-6735 DT denotes the
T21017 6736-6748 NN denotes manufacturer
T21018 6748-6750 POS denotes 's
T21019 6751-6766 NN denotes recommendations
T21020 6767-6770 CC denotes and
T21021 6771-6780 VB denotes subjected
T21022 6781-6783 TO denotes to
T21023 6784-6788 NN denotes FACS
T21024 6789-6790 -LRB- denotes (
T21025 6790-6795 NN denotes EPICS
T21026 6796-6802 NN denotes XL-MCL
T21027 6802-6803 -RRB- denotes )
T21028 6805-6808 IN denotes For
T21029 6809-6813 NN denotes cell
T21030 6814-6821 NN denotes surface
T21031 6822-6828 NN denotes marker
T21032 6829-6837 NN denotes staining
T21033 6837-6838 -COMMA- denotes ,
T21034 6839-6844 NN denotes cells
T21035 6845-6849 VB denotes were
T21036 6850-6859 VB denotes incubated
T21037 6860-6863 IN denotes for
T21038 6864-6866 CD denotes 20
T21039 6867-6870 NN denotes min
T21040 6871-6873 IN denotes at
T21041 6874-6877 NN denotes 4°C
T21042 6878-6880 IN denotes in
T21043 6881-6889 NN denotes staining
T21044 6890-6896 NN denotes buffer
T21045 6897-6901 IN denotes with
T21046 6902-6905 DT denotes the
T21047 6906-6915 VB denotes following
T21048 6916-6926 NN denotes antibodies
T21049 6926-6927 -COLON- denotes :
T21050 6928-6941 NN denotes anti–CD152-PE
T21051 6942-6943 -LRB- denotes (
T21052 6943-6949 NN denotes CTLA-4
T21053 6949-6950 -COLON- denotes ;
T21054 6951-6953 NN denotes BD
T21055 6953-6954 -RRB- denotes )
T21056 6954-6955 -COMMA- denotes ,
T21057 6956-6965 NN denotes anti–PD-1
T21058 6966-6967 -LRB- denotes (
T21059 6967-6979 NN denotes eBiosciences
T21060 6979-6980 -RRB- denotes )
T21061 6980-6981 -COMMA- denotes ,
T21062 6982-6991 NN denotes anti-GITR
T21063 6992-6993 -LRB- denotes (
T21064 6993-6994 NNP denotes R
T21065 6995-6996 CC denotes &
T21066 6997-6998 NNP denotes D
T21067 6999-7006 NNP denotes Systems
T21068 7006-7007 -RRB- denotes )
T21069 7007-7008 -COMMA- denotes ,
T21070 7009-7018 NN denotes anti-CD69
T21071 7019-7020 -LRB- denotes (
T21072 7020-7027 NNP denotes Beckman
T21073 7028-7035 NNP denotes Coulter
T21074 7035-7036 -RRB- denotes )
T21075 7036-7037 -COMMA- denotes ,
T21076 7038-7048 NN denotes anti-CD103
T21077 7049-7050 -LRB- denotes (
T21078 7050-7064 NN denotes DakoCytomation
T21079 7064-7065 -RRB- denotes )
T21080 7065-7066 -COMMA- denotes ,
T21081 7067-7077 NN denotes anti-CD62L
T21082 7078-7079 -LRB- denotes (
T21083 7079-7086 NNP denotes Beckman
T21084 7087-7094 NNP denotes Coulter
T21085 7094-7095 -RRB- denotes )
T21086 7095-7096 -COMMA- denotes ,
T21087 7097-7099 CC denotes or
T21088 7100-7111 JJ denotes anti-HLA-DR
T21089 7112-7113 -LRB- denotes (
T21090 7113-7120 NNP denotes Beckman
T21091 7121-7128 NNP denotes Coulter
T21092 7128-7129 -RRB- denotes )
T21093 7131-7134 DT denotes The
T21094 7135-7143 NN denotes controls
T21095 7144-7148 VB denotes were
T21096 7149-7153 NN denotes FITC
T21097 7153-7154 -COMMA- denotes ,
T21098 7155-7157 NN denotes PE
T21099 7157-7158 -COMMA- denotes ,
T21100 7159-7161 CC denotes or
T21101 7162-7176 JJ denotes ECD-conjugated
T21102 7177-7182 NN denotes mouse
T21103 7183-7187 NN denotes IgG1
T21104 7188-7190 CC denotes or
T21105 7191-7194 NN denotes rat
T21106 7195-7200 NN denotes IgG2a
T21107 7202-7205 IN denotes For
T21108 7206-7214 NN denotes staining
T21109 7215-7217 IN denotes of
T21110 7218-7223 NN denotes mouse
T21111 7224-7229 NN denotes cells
T21112 7229-7230 -COMMA- denotes ,
T21113 7231-7234 DT denotes the
T21114 7235-7244 VB denotes following
T21115 7245-7249 NN denotes mAbs
T21116 7250-7254 IN denotes from
T21117 7255-7257 NN denotes BD
T21118 7258-7269 NN denotes Biosciences
T21119 7270-7274 VB denotes were
T21120 7275-7279 VB denotes used
T21121 7280-7289 VB denotes following
T21122 7290-7298 JJ denotes standard
T21123 7299-7309 NN denotes techniques
T21124 7310-7312 IN denotes as
T21125 7313-7322 VB denotes described
T21126 7323-7328 IN denotes above
T21127 7328-7329 -COLON- denotes :
T21128 7330-7338 NN denotes anti-CD3
T21129 7338-7339 -COMMA- denotes ,
T21130 7340-7348 NN denotes anti-CD4
T21131 7348-7349 -COMMA- denotes ,
T21132 7350-7353 CC denotes and
T21133 7354-7363 NN denotes anti-CD25
T21134 7365-7380 JJ denotes Anti-FcγRII/III
T21135 7381-7389 NN denotes antibody
T21136 7390-7391 -LRB- denotes (
T21137 7391-7396 NN denotes 2.4G2
T21138 7396-7397 -COMMA- denotes ,
T21139 7398-7402 NN denotes ATCC
T21140 7402-7403 -RRB- denotes )
T21141 7404-7407 VB denotes was
T21142 7408-7416 VB denotes included
T21143 7417-7419 IN denotes in
T21144 7420-7423 DT denotes all
T21145 7424-7433 NN denotes stainings
T21146 7434-7436 TO denotes to
T21147 7437-7443 VB denotes reduce
T21148 7444-7455 JJ denotes nonspecific
T21149 7456-7464 NN denotes antibody
T21150 7465-7472 NN denotes binding
T21151 7474-7476 TO denotes To
T21152 7477-7484 VB denotes isolate
T21153 7485-7490 JJ denotes naive
T21154 7491-7497 JJ denotes murine
T21155 7498-7501 NN denotes CD4
T21156 7502-7503 NN denotes T
T21157 7504-7509 NN denotes cells
T21158 7510-7514 IN denotes from
T21159 7515-7521 JJ denotes murine
T21160 7522-7529 NN denotes DO11.10
T21161 7530-7532 CC denotes or
T21162 7533-7550 NN denotes DO11.10xCD2-GATA3
T21163 7551-7552 NN denotes T
T21164 7553-7558 NN denotes cells
T21165 7558-7559 -COMMA- denotes ,
T21166 7560-7565 NN denotes cells
T21167 7566-7570 VB denotes were
T21168 7571-7578 VB denotes stained
T21169 7579-7583 IN denotes with
T21170 7584-7598 NN denotes anti-CD25-FITC
T21171 7598-7599 -COMMA- denotes ,
T21172 7600-7613 NN denotes anti-CD62L-PE
T21173 7613-7614 -COMMA- denotes ,
T21174 7615-7618 CC denotes and
T21175 7619-7631 NN denotes anti-CD4-APC
T21176 7632-7637 JJ denotes prior
T21177 7638-7640 TO denotes to
T21178 7641-7648 NN denotes sorting
T21179 7650-7654 NN denotes Dead
T21180 7655-7660 NN denotes cells
T21181 7661-7665 VB denotes were
T21182 7666-7674 VB denotes excluded
T21183 7675-7679 IN denotes with
T21184 7680-7682 NN denotes 4′
T21185 7682-7683 -DQE- denotes '
T21186 7683-7684 -COMMA- denotes ,
T21187 7684-7710 NN denotes 6-Diamidino-2-phenylindole
T21188 7711-7712 -LRB- denotes (
T21189 7712-7716 NN denotes DAPI
T21190 7716-7717 -RRB- denotes )
T21191 7719-7721 TO denotes To
T21192 7722-7729 VB denotes analyze
T21193 7730-7736 JJ denotes murine
T21194 7737-7742 NN denotes Foxp3
T21195 7743-7753 NN denotes expression
T21196 7754-7756 IN denotes in
T21197 7757-7766 JJ denotes inducible
T21198 7767-7771 NN denotes Treg
T21199 7772-7780 NN denotes cultures
T21200 7780-7781 -COMMA- denotes ,
T21201 7782-7787 NN denotes cells
T21202 7788-7792 VB denotes were
T21203 7793-7800 VB denotes stained
T21204 7801-7816 RB denotes intracellularly
T21205 7817-7821 IN denotes with
T21206 7822-7835 JJ denotes anti-Foxp3-PE
T21207 7836-7845 VB denotes according
T21208 7846-7848 TO denotes to
T21209 7849-7861 NN denotes manufacturer
T21210 7861-7863 POS denotes 's
T21211 7864-7875 NN denotes instruction
T21212 7875-7876 -COMMA- denotes ,
T21213 7877-7879 IN denotes in
T21214 7880-7891 NN denotes conjunction
T21215 7892-7896 IN denotes with
T21216 7897-7909 NN denotes anti-CD4-APC
T21217 7910-7913 CC denotes and
T21218 7914-7923 NN denotes LIVE/DEAD
T21219 7924-7931 JJ denotes fixable
T21220 7932-7936 JJ denotes dead
T21221 7937-7941 NN denotes cell
T21222 7942-7947 NN denotes stain
T21223 7948-7951 NN denotes kit
T21224 7952-7953 -LRB- denotes (
T21225 7953-7963 NN denotes Invitrogen
T21226 7963-7964 -RRB- denotes )
T21227 7965-7967 TO denotes to
T21228 7968-7980 VB denotes discriminate
T21229 7981-7985 JJ denotes live
T21230 7986-7991 NN denotes cells
T21231 7993-7996 DT denotes All
T21232 7997-8007 JJ denotes monoclonal
T21233 8008-8018 NN denotes antibodies
T21234 8019-8022 IN denotes for
T21235 8023-8029 JJ denotes murine
T21236 8030-8034 NN denotes cell
T21237 8035-8044 NN denotes stainings
T21238 8045-8049 VB denotes were
T21239 8050-8059 VB denotes purchased
T21240 8060-8064 IN denotes from
T21241 8065-8076 NN denotes eBioscience
T21242 8077-8079 CC denotes or
T21243 8080-8082 NN denotes BD
T21244 8083-8094 NNP denotes Biosciences
T21790 8097-8104 NN denotes Western
T21791 8105-8113 NN denotes blotting
T21792 8115-8118 IN denotes For
T21793 8119-8124 NN denotes FOXP3
T21794 8125-8133 NN denotes analysis
T21795 8134-8136 IN denotes on
T21796 8137-8140 DT denotes the
T21797 8141-8148 NN denotes protein
T21798 8149-8154 NN denotes level
T21799 8154-8155 -COMMA- denotes ,
T21800 8156-8157 CD denotes 1
T21801 8158-8159 CC denotes ×
T21802 8160-8163 CD denotes 106
T21803 8164-8173 NN denotes CD4+CD25–
T21804 8174-8179 NN denotes cells
T21805 8180-8184 VB denotes were
T21806 8185-8190 VB denotes lysed
T21807 8191-8194 CC denotes and
T21808 8195-8201 VB denotes loaded
T21809 8202-8206 JJ denotes next
T21810 8207-8209 TO denotes to
T21811 8210-8211 DT denotes a
T21812 8212-8224 JJ denotes protein-mass
T21813 8225-8231 NN denotes ladder
T21814 8232-8233 -LRB- denotes (
T21815 8233-8242 NNP denotes Magicmark
T21816 8242-8243 -COMMA- denotes ,
T21817 8244-8254 NN denotes Invitrogen
T21818 8254-8255 -RRB- denotes )
T21819 8256-8258 IN denotes on
T21820 8259-8260 DT denotes a
T21821 8261-8267 NN denotes NuPAGE
T21822 8268-8272 CD denotes 4–12
T21823 8272-8273 NN denotes %
T21824 8274-8282 JJ denotes bis-tris
T21825 8283-8286 NN denotes gel
T21826 8287-8288 -LRB- denotes (
T21827 8288-8298 NN denotes Invitrogen
T21828 8298-8299 -RRB- denotes )
T21829 8301-8304 DT denotes The
T21830 8305-8313 NN denotes proteins
T21831 8314-8318 VB denotes were
T21832 8319-8333 VB denotes electroblotted
T21833 8334-8338 IN denotes onto
T21834 8339-8340 DT denotes a
T21835 8341-8345 NN denotes PVDF
T21836 8346-8354 NN denotes membrane
T21837 8355-8356 -LRB- denotes (
T21838 8356-8364 NNP denotes Amersham
T21839 8365-8369 NNP denotes Life
T21840 8370-8377 NNP denotes Science
T21841 8377-8378 -RRB- denotes )
T21842 8380-8390 JJ denotes Unspecific
T21843 8391-8398 NN denotes binding
T21844 8399-8402 VB denotes was
T21845 8403-8410 VB denotes blocked
T21846 8411-8415 IN denotes with
T21847 8416-8419 NN denotes BSA
T21848 8419-8420 -COMMA- denotes ,
T21849 8421-8424 CC denotes and
T21850 8425-8428 DT denotes the
T21851 8429-8438 NN denotes membranes
T21852 8439-8443 VB denotes were
T21853 8444-8456 RB denotes subsequently
T21854 8457-8466 VB denotes incubated
T21855 8467-8471 IN denotes with
T21856 8472-8474 DT denotes an
T21857 8475-8476 CD denotes 1
T21858 8476-8477 -COLON- denotes :
T21859 8477-8480 CD denotes 200
T21860 8481-8489 NN denotes dilution
T21861 8490-8492 IN denotes of
T21862 8493-8497 NN denotes goat
T21863 8498-8508 NN denotes anti-FOXP3
T21864 8509-8511 IN denotes in
T21865 8512-8520 VB denotes blocking
T21866 8521-8527 NN denotes buffer
T21867 8528-8529 -LRB- denotes (
T21868 8529-8534 NN denotes Abcam
T21869 8534-8535 -RRB- denotes )
T21870 8536-8545 RB denotes overnight
T21871 8546-8548 IN denotes at
T21872 8549-8550 CD denotes 4
T21873 8551-8554 NNP denotes °C.
T21874 8555-8558 DT denotes The
T21875 8559-8564 NN denotes blots
T21876 8565-8569 VB denotes were
T21877 8570-8579 VB denotes developed
T21878 8580-8585 VB denotes using
T21879 8586-8588 DT denotes an
T21880 8589-8598 JJ denotes anti-goat
T21881 8599-8610 JJ denotes HRP-labeled
T21882 8611-8614 NN denotes mAb
T21883 8615-8616 -LRB- denotes (
T21884 8616-8624 NNP denotes Amersham
T21885 8625-8636 NNP denotes Biosciences
T21886 8636-8637 -RRB- denotes )
T21887 8638-8641 CC denotes and
T21888 8642-8652 VB denotes visualized
T21889 8653-8657 IN denotes with
T21890 8658-8659 DT denotes a
T21891 8660-8663 NN denotes LAS
T21892 8664-8668 CD denotes 1000
T21893 8669-8675 NN denotes camera
T21894 8676-8677 -LRB- denotes (
T21895 8677-8681 NN denotes Fuji
T21896 8681-8682 -RRB- denotes )
T21897 8684-8693 NN denotes Membranes
T21898 8694-8698 VB denotes were
T21899 8699-8708 VB denotes incubated
T21900 8709-8711 IN denotes in
T21901 8712-8721 VB denotes stripping
T21902 8722-8728 NN denotes buffer
T21903 8729-8732 CC denotes and
T21904 8733-8743 JJ denotes re-blocked
T21905 8744-8747 IN denotes for
T21906 8748-8749 CD denotes 1
T21907 8750-8751 NN denotes h
T21908 8753-8756 DT denotes The
T21909 8757-8766 NN denotes membranes
T21910 8767-8771 VB denotes were
T21911 8772-8781 VB denotes re-probed
T21912 8782-8787 VB denotes using
T21913 8788-8798 NN denotes anti-GATA3
T21914 8799-8800 -LRB- denotes (
T21915 8800-8806 NN denotes HG3–31
T21916 8806-8807 -COLON- denotes ;
T21917 8808-8813 NNP denotes Santa
T21918 8814-8818 NNP denotes Cruz
T21919 8819-8832 NNP denotes Biotechnology
T21920 8832-8833 -RRB- denotes )
T21921 8833-8834 -COMMA- denotes ,
T21922 8835-8845 JJ denotes anti-T-bet
T21923 8846-8847 -LRB- denotes (
T21924 8847-8851 NN denotes 4B10
T21925 8851-8852 -COMMA- denotes ,
T21926 8853-8858 NNP denotes Santa
T21927 8859-8863 NNP denotes Cruz
T21928 8864-8877 NNP denotes Biotechnology
T21929 8877-8878 -RRB- denotes )
T21930 8878-8879 -COMMA- denotes ,
T21931 8880-8890 NN denotes anti-GAPDH
T21932 8891-8892 -LRB- denotes (
T21933 8892-8895 NN denotes 6C5
T21934 8895-8896 -COMMA- denotes ,
T21935 8897-8903 NNP denotes Ambion
T21936 8903-8904 -RRB- denotes )
T21937 8904-8905 -COMMA- denotes ,
T21938 8906-8924 NN denotes anti-phospho-SMAD2
T21939 8925-8926 -LRB- denotes (
T21940 8926-8931 NN denotes 138D4
T21941 8931-8932 -RRB- denotes )
T21942 8932-8933 -COMMA- denotes ,
T21943 8934-8952 NN denotes anti-phospho-STAT6
T21944 8953-8954 -LRB- denotes (
T21945 8954-8957 NN denotes 5A4
T21946 8957-8958 -RRB- denotes )
T21947 8958-8959 -COMMA- denotes ,
T21948 8960-8963 CC denotes and
T21949 8964-8974 JJ denotes anti-STAT6
T21950 8975-8976 -LRB- denotes (
T21951 8976-8980 NNP denotes Cell
T21952 8981-8990 NNP denotes Signaling
T21953 8991-9001 NNP denotes Technology
T21954 9001-9002 -RRB- denotes )
T21955 9002-9003 -COMMA- denotes ,
T22260 9005-9019 NN denotes Administration
T22261 9020-9022 IN denotes of
T22262 9023-9032 NN denotes cytokines
T22263 9033-9036 CC denotes and
T22264 9037-9047 NN denotes antibodies
T22265 9048-9050 FW denotes in
T22266 9051-9055 FW denotes vivo
T22267 9057-9061 NN denotes Age-
T22268 9062-9065 CC denotes and
T22269 9066-9080 JJ denotes gender-matched
T22270 9081-9087 JJ denotes normal
T22271 9088-9090 NN denotes B6
T22272 9091-9095 NN denotes mice
T22273 9096-9104 VB denotes received
T22274 9105-9110 DT denotes every
T22275 9111-9116 JJ denotes other
T22276 9117-9120 NN denotes day
T22277 9121-9136 JJ denotes intraperitoneal
T22278 9137-9138 -LRB- denotes (
T22279 9138-9140 JJ denotes ip
T22280 9140-9141 -RRB- denotes )
T22281 9142-9152 NN denotes injections
T22282 9153-9155 IN denotes of
T22283 9156-9159 NNP denotes PBS
T22284 9159-9160 -COMMA- denotes ,
T22285 9161-9164 CD denotes 1.5
T22286 9165-9167 NN denotes μg
T22287 9168-9174 NN denotes rmIL-4
T22288 9174-9175 -COMMA- denotes ,
T22289 9176-9178 CD denotes 50
T22290 9179-9181 CC denotes μg
T22291 9182-9191 JJ denotes anti-IL-4
T22292 9192-9195 NN denotes mAb
T22293 9196-9197 -LRB- denotes (
T22294 9197-9202 NN denotes 11B11
T22295 9203-9205 CC denotes or
T22296 9206-9212 NN denotes MAB404
T22297 9212-9213 -RRB- denotes )
T22298 9213-9214 -COMMA- denotes ,
T22299 9215-9217 CC denotes or
T22300 9218-9219 DT denotes a
T22301 9220-9227 NN denotes mixture
T22302 9228-9230 IN denotes of
T22303 9231-9234 CD denotes 1.5
T22304 9235-9237 NN denotes μg
T22305 9238-9244 NN denotes rmIL-4
T22306 9245-9249 CC denotes plus
T22307 9250-9252 CD denotes 50
T22308 9253-9255 CC denotes μg
T22309 9256-9265 JJ denotes anti-IL-4
T22310 9266-9269 NN denotes mAb
T22311 9270-9271 -LRB- denotes (
T22312 9271-9276 NN denotes 11B11
T22313 9277-9279 CC denotes or
T22314 9280-9286 NN denotes MAB404
T22315 9286-9287 -RRB- denotes )
T22316 9288-9291 IN denotes for
T22317 9292-9293 CD denotes 7
T22318 9294-9295 NN denotes d
T22319 9297-9307 RB denotes Thereafter
T22320 9307-9308 -COMMA- denotes ,
T22321 9309-9315 NN denotes spleen
T22322 9316-9319 CC denotes and
T22323 9320-9325 NN denotes lymph
T22324 9326-9330 NN denotes node
T22325 9331-9336 NN denotes cells
T22326 9337-9341 VB denotes were
T22327 9342-9350 VB denotes analyzed
T22328 9351-9353 IN denotes by
T22329 9354-9358 NN denotes flow
T22330 9359-9368 NN denotes cytometry
T22331 9369-9372 IN denotes for
T22332 9373-9376 NN denotes CD3
T22333 9376-9377 -COMMA- denotes ,
T22334 9378-9381 NN denotes CD4
T22335 9381-9382 -COMMA- denotes ,
T22336 9383-9386 CC denotes and
T22337 9387-9391 NN denotes CD25
T22338 9392-9402 NN denotes expression
T22339 9404-9407 DT denotes The
T22340 9408-9418 JJ denotes anti-mouse
T22341 9419-9423 NN denotes IL-4
T22342 9424-9427 NN denotes mAb
T22343 9428-9434 NN denotes MAB404
T22344 9435-9438 VB denotes was
T22345 9439-9447 VB denotes obtained
T22346 9448-9452 IN denotes from
T22347 9453-9454 NNP denotes R
T22348 9454-9455 CC denotes &
T22349 9455-9456 NNP denotes D
T22350 9457-9464 NNP denotes Systems
T22351 9464-9465 -COMMA- denotes ,
T22352 9466-9469 DT denotes the
T22353 9470-9476 JJ denotes second
T22354 9477-9487 JJ denotes anti-mouse
T22355 9488-9492 NN denotes IL-4
T22356 9493-9496 NN denotes mAb
T22357 9497-9502 NN denotes 11B11
T22358 9503-9506 VB denotes was
T22359 9507-9516 VB denotes purchased
T22360 9517-9521 IN denotes from
T22361 9522-9533 NN denotes eBioscience
T22574 9536-9540 NN denotes ChIP
T22575 9542-9546 NN denotes ChIP
T22576 9547-9555 NN denotes analysis
T22577 9556-9559 VB denotes was
T22578 9560-9569 VB denotes performed
T22579 9570-9579 VB denotes according
T22580 9580-9582 TO denotes to
T22581 9583-9586 DT denotes the
T22582 9587-9599 NN denotes manufacturer
T22583 9599-9601 POS denotes 's
T22584 9602-9610 NN denotes protocol
T22585 9611-9612 -LRB- denotes (
T22586 9612-9619 NNP denotes Upstate
T22587 9620-9633 NNP denotes Biotechnology
T22588 9633-9634 -RRB- denotes )
T22589 9635-9639 IN denotes with
T22590 9640-9643 DT denotes the
T22591 9644-9653 VB denotes following
T22592 9654-9668 NN denotes modifications.
T22593 9669-9674 NN denotes iTreg
T22594 9675-9678 CC denotes and
T22595 9679-9682 NN denotes Th2
T22596 9683-9688 NN denotes cells
T22597 9689-9693 VB denotes were
T22598 9694-9699 VB denotes fixed
T22599 9700-9704 IN denotes with
T22600 9705-9706 CD denotes 1
T22601 9706-9707 NN denotes %
T22602 9708-9720 NN denotes formaldehyde
T22603 9721-9724 IN denotes for
T22604 9725-9727 CD denotes 10
T22605 9728-9731 NN denotes min
T22606 9732-9734 IN denotes at
T22607 9735-9739 NN denotes room
T22608 9740-9751 NN denotes temperature
T22609 9753-9756 DT denotes The
T22610 9757-9766 NN denotes chromatin
T22611 9767-9770 VB denotes was
T22612 9771-9778 VB denotes sheared
T22613 9779-9781 TO denotes to
T22614 9782-9790 CD denotes 200-1000
T22615 9791-9793 NN denotes bp
T22616 9794-9796 IN denotes of
T22617 9797-9803 NN denotes length
T22618 9804-9806 IN denotes by
T22619 9807-9817 NN denotes sonication
T22620 9818-9822 IN denotes with
T22621 9823-9827 CD denotes five
T22622 9828-9834 NN denotes pulses
T22623 9835-9837 IN denotes of
T22624 9838-9840 CD denotes 10
T22625 9841-9842 NN denotes s
T22626 9843-9845 IN denotes at
T22627 9846-9848 CD denotes 30
T22628 9848-9849 NN denotes %
T22629 9850-9855 NN denotes power
T22630 9856-9857 -LRB- denotes (
T22631 9857-9865 NN denotes Bandelin
T22632 9865-9866 -RRB- denotes )
T22633 9868-9871 DT denotes The
T22634 9872-9881 NN denotes chromatin
T22635 9882-9885 VB denotes was
T22636 9886-9897 JJ denotes pre-cleared
T22637 9898-9901 IN denotes for
T22638 9902-9903 CD denotes 2
T22639 9904-9905 NN denotes h
T22640 9906-9910 IN denotes with
T22641 9911-9917 JJ denotes normal
T22642 9918-9923 NN denotes mouse
T22643 9924-9927 NN denotes IgG
T22644 9928-9933 NN denotes beads
T22645 9934-9937 CC denotes and
T22646 9938-9942 RB denotes then
T22647 9943-9952 VB denotes incubated
T22648 9953-9957 IN denotes with
T22649 9958-9976 JJ denotes anti-GATA3-agarose
T22650 9977-9982 NN denotes beads
T22651 9983-9984 -LRB- denotes (
T22652 9984-9990 NN denotes HG3–31
T22653 9990-9991 -COLON- denotes ;
T22654 9992-9997 NNP denotes Santa
T22655 9998-10002 NNP denotes Cruz
T22656 10003-10016 NNP denotes Biotechnology
T22657 10016-10017 -RRB- denotes )
T22658 10018-10021 IN denotes for
T22659 10022-10023 CD denotes 2
T22660 10024-10025 NN denotes h
T22661 10027-10034 NN denotes Washing
T22662 10035-10038 CC denotes and
T22663 10039-10046 NN denotes elution
T22664 10047-10054 NN denotes buffers
T22665 10055-10059 VB denotes were
T22666 10060-10064 VB denotes used
T22667 10065-10074 VB denotes according
T22668 10075-10077 TO denotes to
T22669 10078-10081 DT denotes the
T22670 10082-10090 NN denotes protocol
T22671 10091-10093 IN denotes of
T22672 10094-10101 NNP denotes Upstate
T22673 10102-10116 NNP denotes Biotechnology.
T22674 10117-10127 NNP denotes Crosslinks
T22675 10128-10132 VB denotes were
T22676 10133-10141 VB denotes reversed
T22677 10142-10144 IN denotes by
T22678 10145-10155 NN denotes incubation
T22679 10156-10158 IN denotes at
T22680 10159-10161 CD denotes 65
T22681 10162-10164 NN denotes °C
T22682 10165-10168 IN denotes for
T22683 10169-10170 CD denotes 4
T22684 10171-10172 NN denotes h
T22685 10173-10175 IN denotes in
T22686 10176-10179 DT denotes the
T22687 10180-10188 NN denotes presence
T22688 10189-10191 IN denotes of
T22689 10192-10195 CD denotes 0.2
T22690 10196-10197 NN denotes M
T22691 10198-10202 NN denotes NaCl
T22692 10202-10203 -COMMA- denotes ,
T22693 10204-10207 CC denotes and
T22694 10208-10211 DT denotes the
T22695 10212-10215 NN denotes DNA
T22696 10216-10219 VB denotes was
T22697 10220-10228 VB denotes purified
T22698 10229-10231 IN denotes by
T22699 10232-10249 NN denotes phenol/chloroform
T22700 10250-10260 NN denotes extraction
T22701 10262-10265 DT denotes The
T22702 10266-10272 NN denotes amount
T22703 10273-10275 IN denotes of
T22704 10276-10279 NN denotes DNA
T22705 10280-10283 VB denotes was
T22706 10284-10294 VB denotes determined
T22707 10295-10297 IN denotes by
T22708 10298-10310 JJ denotes conventional
T22709 10311-10314 NN denotes PCR
T22710 10316-10319 DT denotes The
T22711 10320-10323 NN denotes PCR
T22712 10324-10333 VB denotes addressed
T22713 10334-10337 IN denotes for
T22714 10338-10341 DT denotes the
T22715 10342-10347 NN denotes FOXP3
T22716 10348-10356 NN denotes promoter
T22717 10357-10363 NN denotes region
T22718 10364-10368 CD denotes −246
T22719 10369-10371 TO denotes to
T22720 10372-10376 CD denotes −511
T22721 10377-10380 CC denotes and
T22722 10381-10384 VB denotes was
T22723 10385-10394 VB denotes performed
T22724 10395-10400 VB denotes using
T22725 10401-10404 DT denotes the
T22726 10405-10414 VB denotes following
T22727 10415-10422 NN denotes primers
T22728 10422-10423 -COLON- denotes :
T22729 10424-10442 JJ denotes 5′-gtgccctttacgagt
T22730 10443-10452 NN denotes catctg-3′
T22731 10453-10456 CC denotes and
T22732 10457-10484 NN denotes 5′-gtgccctttacgagtcatctg-3′
T22733 10486-10489 DT denotes The
T22734 10490-10493 NN denotes PCR
T22735 10494-10502 NN denotes products
T22736 10503-10507 VB denotes were
T22737 10508-10518 VB denotes visualized
T22738 10519-10524 VB denotes using
T22739 10525-10527 DT denotes an
T22740 10528-10536 NN denotes ethidium
T22741 10537-10544 NN denotes bromide
T22742 10545-10548 NN denotes gel
T22975 10551-10560 JJ denotes Pull-down
T22976 10561-10566 NN denotes assay
T22977 10568-10572 JJ denotes CD4+
T22978 10573-10574 NN denotes T
T22979 10575-10580 NN denotes cells
T22980 10581-10585 VB denotes were
T22981 10586-10596 VB denotes stimulated
T22982 10597-10601 IN denotes with
T22983 10602-10605 NN denotes PMA
T22984 10606-10609 CC denotes and
T22985 10610-10619 NN denotes ionomycin
T22986 10620-10623 IN denotes for
T22987 10624-10625 CD denotes 2
T22988 10626-10627 NN denotes h
T22989 10628-10630 IN denotes at
T22990 10631-10636 NNP denotes 37°C.
T22991 10637-10640 DT denotes The
T22992 10641-10646 NN denotes cells
T22993 10647-10651 VB denotes were
T22994 10652-10660 VB denotes pelleted
T22995 10660-10661 -COMMA- denotes ,
T22996 10662-10673 VB denotes resuspended
T22997 10674-10676 IN denotes in
T22998 10677-10683 NN denotes buffer
T22999 10684-10685 NN denotes C
T23000 10686-10687 -LRB- denotes (
T23001 10687-10689 CD denotes 20
T23002 10690-10692 NN denotes mM
T23003 10693-10698 NN denotes HEPES
T23004 10699-10700 -LRB- denotes [
T23005 10700-10702 NN denotes pH
T23006 10703-10706 CD denotes 7.9
T23007 10706-10707 -RRB- denotes ]
T23008 10707-10708 -COMMA- denotes ,
T23009 10709-10712 CD denotes 420
T23010 10713-10715 NN denotes mM
T23011 10716-10720 NN denotes NaCl
T23012 10720-10721 -COMMA- denotes ,
T23013 10722-10725 CD denotes 1.5
T23014 10726-10728 NN denotes mM
T23015 10729-10734 NN denotes MgCl2
T23016 10734-10735 -COMMA- denotes ,
T23017 10736-10739 CD denotes 0.2
T23018 10740-10742 NN denotes mM
T23019 10743-10747 NN denotes EDTA
T23020 10747-10748 -COMMA- denotes ,
T23021 10749-10750 CD denotes 1
T23022 10751-10753 NN denotes mM
T23023 10754-10757 NN denotes DTT
T23024 10757-10758 -COMMA- denotes ,
T23025 10759-10767 NN denotes protease
T23026 10768-10778 NN denotes inhibitors
T23027 10779-10780 -LRB- denotes [
T23028 10780-10785 NNP denotes Sigma
T23029 10785-10786 -RRB- denotes ]
T23030 10786-10789 CC denotes . a
T23031 10789-10792 CD denotes nd
T23032 10792-10793 NN denotes 0
T23033 10793-10798 NN denotes .1% N
T23034 10798-10799 -RRB- denotes P
T23035 10799-10802 CC denotes -40
T23036 10802-10807 VB denotes ) and
T23037 10808-10810 IN denotes ly
T23038 10810-10813 NN denotes sed
T23039 10814-10817 IN denotes on
T23040 10817-10819 CD denotes ic
T23041 10819-10822 NN denotes e f
T23042 10833-10842 JJ denotes Insoluble
T23043 10843-10851 NN denotes material
T23044 10852-10855 VB denotes was
T23045 10856-10863 VB denotes removed
T23046 10864-10866 IN denotes by
T23047 10867-10881 NN denotes centrifugation
T23048 10883-10886 DT denotes The
T23049 10887-10898 NN denotes supernatant
T23050 10899-10902 VB denotes was
T23051 10903-10910 VB denotes diluted
T23052 10911-10912 CD denotes 1
T23053 10912-10913 -COLON- denotes :
T23054 10913-10914 CD denotes 3
T23055 10915-10919 IN denotes with
T23056 10920-10926 NN denotes buffer
T23057 10927-10928 NN denotes D
T23058 10929-10930 -LRB- denotes (
T23059 10930-10932 IN denotes as
T23060 10933-10939 NN denotes buffer
T23061 10940-10941 NN denotes C
T23062 10941-10942 -COMMA- denotes ,
T23063 10943-10946 CC denotes but
T23064 10947-10954 IN denotes without
T23065 10955-10959 NN denotes NaCl
T23066 10959-10960 -RRB- denotes )
T23067 10962-10965 DT denotes The
T23068 10966-10973 NN denotes lysates
T23069 10974-10978 VB denotes were
T23070 10979-10988 VB denotes incubated
T23071 10989-10993 IN denotes with
T23072 10994-10996 CD denotes 10
T23073 10997-10999 NN denotes μg
T23074 11000-11002 IN denotes of
T23075 11003-11007 NN denotes poly
T23076 11007-11008 -LRB- denotes (
T23077 11008-11013 NN denotes dI-dC
T23078 11013-11014 -RRB- denotes )
T23079 11015-11016 -LRB- denotes (
T23080 11016-11021 NN denotes Sigma
T23081 11021-11022 -RRB- denotes )
T23082 11023-11026 CC denotes and
T23083 11027-11029 CD denotes 70
T23084 11030-11032 NN denotes μl
T23085 11033-11035 IN denotes of
T23086 11036-11056 JJ denotes streptavidin-agarose
T23087 11057-11058 -LRB- denotes (
T23088 11058-11066 NNP denotes Amersham
T23089 11067-11078 NNP denotes Biosciences
T23090 11078-11079 -RRB- denotes )
T23091 11080-11088 VB denotes carrying
T23092 11089-11101 VB denotes biotinylated
T23093 11102-11118 NN denotes oligonucleotides
T23094 11118-11119 -COMMA- denotes ,
T23095 11120-11123 IN denotes for
T23096 11124-11125 CD denotes 3
T23097 11126-11127 NN denotes h
T23098 11128-11130 IN denotes at
T23099 11131-11132 CD denotes 4
T23100 11133-11136 NNP denotes °C.
T23101 11137-11140 DT denotes The
T23102 11141-11146 NN denotes beads
T23103 11147-11151 VB denotes were
T23104 11152-11158 VB denotes washed
T23105 11159-11164 RB denotes twice
T23106 11165-11169 IN denotes with
T23107 11170-11176 NN denotes buffer
T23108 11177-11178 NN denotes C
T23109 11178-11179 -COLON- denotes :
T23110 11179-11180 NN denotes D
T23111 11181-11182 -LRB- denotes (
T23112 11182-11183 CD denotes 1
T23113 11183-11184 -COLON- denotes :
T23114 11184-11185 CD denotes 3
T23115 11185-11186 -RRB- denotes )
T23116 11187-11190 CC denotes and
T23117 11191-11202 VB denotes resuspended
T23118 11203-11205 IN denotes in
T23119 11206-11220 JJ denotes DTT-containing
T23120 11221-11228 NN denotes loading
T23121 11229-11235 NN denotes buffer
T23122 11236-11237 -LRB- denotes (
T23123 11237-11243 NN denotes NuPAGE
T23124 11243-11244 -COLON- denotes ;
T23125 11245-11255 NN denotes Invitrogen
T23126 11255-11256 -RRB- denotes )
T23127 11256-11257 -COMMA- denotes ,
T23128 11258-11264 VB denotes heated
T23129 11265-11267 TO denotes to
T23130 11268-11270 CD denotes 70
T23131 11271-11273 NN denotes °C
T23132 11274-11277 IN denotes for
T23133 11278-11280 CD denotes 10
T23134 11281-11284 NN denotes min
T23135 11284-11285 -COMMA- denotes ,
T23136 11286-11289 CC denotes and
T23137 11290-11293 DT denotes the
T23138 11294-11301 NN denotes eluants
T23139 11302-11304 IN denotes on
T23140 11305-11306 DT denotes a
T23141 11307-11313 NN denotes NuPAGE
T23142 11314-11318 CD denotes 4–12
T23143 11318-11319 NN denotes %
T23144 11320-11328 JJ denotes bis-tris
T23145 11329-11332 NN denotes gel
T23146 11333-11334 -LRB- denotes (
T23147 11334-11344 NN denotes Invitrogen
T23148 11344-11345 -RRB- denotes )
T23149 11347-11350 DT denotes The
T23150 11351-11359 NN denotes proteins
T23151 11360-11364 VB denotes were
T23152 11365-11379 VB denotes electroblotted
T23153 11380-11384 IN denotes onto
T23154 11385-11386 DT denotes a
T23155 11387-11391 NN denotes PVDF
T23156 11392-11400 NN denotes membrane
T23157 11401-11402 -LRB- denotes (
T23158 11402-11410 NNP denotes Amersham
T23159 11411-11422 NNP denotes Biosciences
T23160 11422-11423 -RRB- denotes )
T23161 11424-11427 CC denotes and
T23162 11428-11436 VB denotes detected
T23163 11437-11442 VB denotes using
T23164 11443-11445 DT denotes an
T23165 11446-11456 JJ denotes anti-GATA3
T23166 11457-11460 NN denotes mAb
T23167 11461-11462 -LRB- denotes (
T23168 11462-11467 NNP denotes Santa
T23169 11468-11472 NNP denotes Cruz
T23170 11473-11486 NNP denotes Biotechnology
T23171 11486-11487 -RRB- denotes )
T23172 11489-11500 VB denotes Accumulated
T23173 11501-11508 NN denotes signals
T23174 11509-11513 VB denotes were
T23175 11514-11522 VB denotes analyzed
T23176 11523-11528 VB denotes using
T23177 11529-11533 NN denotes AIDA
T23178 11534-11542 NN denotes software
T23179 11543-11544 -LRB- denotes (
T23180 11544-11551 NN denotes Raytest
T23181 11551-11552 -RRB- denotes )
T19522 3713-3720 NN denotes Cloning
T19523 3721-3723 IN denotes of
T19524 3724-3727 DT denotes the
T19525 3728-3733 NN denotes FOXP3
T19526 3734-3742 NN denotes promoter
T19527 3743-3746 CC denotes and
T19528 3747-3759 NN denotes construction
T19529 3760-3762 IN denotes of
T19530 3763-3769 JJ denotes mutant
T19531 3770-3780 NN denotes constructs
T19532 3782-3785 DT denotes The
T19533 3786-3791 NN denotes FOXP3
T19534 3792-3800 NN denotes promoter
T19535 3801-3804 VB denotes was
T19536 3805-3811 VB denotes cloned
T20051 4825-4828 NN denotes RNA
T20052 4829-4838 NN denotes isolation
T20053 4839-4842 CC denotes and
T20054 4843-4847 NN denotes cDNA
T20055 4848-4857 NN denotes synthesis
T20056 4859-4862 NN denotes RNA
T20057 4863-4866 VB denotes was
T20058 4867-4875 VB denotes isolated
T20059 4876-4881 VB denotes using
T20060 4882-4885 DT denotes the
T20061 4886-4892 NNP denotes RNeasy
T20062 4893-4897 NNP denotes Mini
T20063 4898-4901 NNP denotes Kit
T20064 4902-4903 -LRB- denotes (
T20065 4903-4909 NN denotes Qiagen
T20066 4909-4910 -RRB- denotes )
T20067 4911-4920 VB denotes according
T20068 4921-4923 TO denotes to
T20069 4924-4927 DT denotes the
T20070 4928-4940 NN denotes manufacturer
T20071 4940-4942 POS denotes 's
T20072 4943-4951 NN denotes protocol
T20073 4953-4960 JJ denotes Reverse
T20074 4961-4974 NN denotes transcription
T20075 4975-4977 IN denotes of
T20076 4978-4983 JJ denotes human
T20077 4984-4991 NN denotes samples
T20078 4992-4995 VB denotes was
T20079 4996-5005 VB denotes performed
R13418 T17781 T17779 arg1Of mice,Normal
R13419 T17781 T17780 arg1Of mice,B6
R13420 T17781 T17782 arg1Of mice,were
R13421 T17781 T17783 arg2Of mice,purchased
R13423 T17783 T17782 arg2Of purchased,were
R13428 T17790 T17788 arg2Of Harbor,(
R13429 T17790 T17789 arg1Of Harbor,Bar
R13430 T17790 T17791 arg1Of Harbor,","
R13431 T17792 T17791 arg2Of Maine,","
R13432 T17793 T17788 arg3Of ),(
R13433 T17796 T17794 arg1Of mice,Transgenic
R13434 T17796 T17795 arg1Of mice,DO11.10
R13435 T17796 T17797 arg1Of mice,","
R13436 T17796 T17798 arg1Of mice,expressing
R13437 T17796 T17812 arg1Of mice,were
R13438 T17796 T17813 arg2Of mice,backcrossed
R13439 T17802 T17798 arg2Of receptor,expressing
R13440 T17802 T17799 arg1Of receptor,a
R13441 T17802 T17800 arg1Of receptor,T
R13442 T17802 T17801 arg1Of receptor,cell
R13443 T17802 T17803 arg1Of receptor,for
R13444 T17805 T17803 arg2Of peptide,for
R13445 T17805 T17804 arg1Of peptide,OVA323–339
R13446 T17805 T17806 arg1Of peptide,in
R13447 T17808 T17806 arg2Of context,in
R13448 T17808 T17807 arg1Of context,the
R13449 T17808 T17809 arg1Of context,of
R13450 T17810 T17809 arg2Of H-2d,of
R13451 T17813 T17811 arg1Of backcrossed,","
R13452 T17813 T17812 arg2Of backcrossed,were
R13453 T17813 T17814 arg1Of backcrossed,with
R13454 T17813 T17830 arg1Of backcrossed,[
R13455 T17813 T17833 arg1Of backcrossed,","
R13456 T17813 T17834 modOf backcrossed,resulting
R13457 T17815 T17814 arg2Of mice,with
R13458 T17815 T17816 arg1Of mice,expressing
R13459 T17815 T17818 arg1Of mice,","
R13460 T17815 T17819 arg2Of mice,driven
R13461 T17817 T17816 arg2Of GATA-3,expressing
R13462 T17826 T17819 arg1Of region,driven
R13463 T17826 T17820 arg2Of region,by
R13464 T17826 T17821 arg1Of region,the
R13465 T17826 T17822 arg1Of region,human
R13466 T17826 T17823 arg1Of region,CD2
R13467 T17826 T17824 arg1Of region,locus
R13468 T17826 T17825 arg1Of region,control
R13469 T17826 T17827 arg1Of region,(
R13470 T17828 T17827 arg2Of CD2-GATA3,(
R13471 T17829 T17827 arg3Of ),(
R13472 T17831 T17830 arg2Of 22,[
R13473 T17832 T17830 arg3Of ],[
R13474 T17834 T17835 arg1Of resulting,in
R13475 T17837 T17835 arg2Of mice,in
R13476 T17837 T17836 arg1Of mice,DO11.10xCD2-GATA3
R13477 T17838 T17839 arg2Of Mice,used
R13478 T17838 T17842 arg1Of Mice,were
R13479 T17838 T17843 arg2Of Mice,backcrossed
R13480 T17838 T17855 arg2Of Mice,used
R13481 T17839 T17840 arg1Of used,for
R13482 T17841 T17840 arg2Of experiments,for
R13483 T17843 T17844 arg1Of backcrossed,on
R13484 T17843 T17854 arg1Of backcrossed,and
R13485 T17847 T17844 arg2Of background,on
R13486 T17847 T17845 arg1Of background,a
R13487 T17847 T17846 arg1Of background,BALB/c
R13488 T17847 T17848 arg1Of background,for
R13489 T17850 T17848 arg2Of minimum,for
R13490 T17850 T17849 arg1Of minimum,a
R13491 T17850 T17851 arg1Of minimum,of
R13492 T17853 T17851 arg2Of generations,of
R13493 T17853 T17852 arg1Of generations,eight
R13494 T17854 T17842 arg2Of and,were
R13495 T17855 T17854 arg2Of used,and
R13496 T17855 T17856 arg1Of used,at
R13497 T17858 T17856 arg2Of age,at
R13498 T17858 T17857 arg1Of age,an
R13499 T17858 T17859 arg1Of age,of
R13500 T17861 T17859 arg2Of wk,of
R13501 T17861 T17860 arg1Of wk,8–12
R13502 T17862 T17863 arg1Of Mice,were
R13503 T17862 T17864 arg2Of Mice,housed
R13504 T17864 T17863 arg2Of housed,were
R13505 T17864 T17865 arg1Of housed,under
R13506 T17864 T17869 arg1Of housed,and
R13507 T17868 T17865 arg2Of conditions,under
R13508 T17868 T17866 arg1Of conditions,specific
R13509 T17868 T17867 arg1Of conditions,pathogen-free
R13510 T17872 T17870 arg1Of studies,all
R13511 T17872 T17871 arg1Of studies,animal
R13512 T17872 T17873 arg1Of studies,were
R13513 T17872 T17874 arg2Of studies,performed
R13514 T17874 T17869 arg2Of performed,and
R13515 T17874 T17873 arg2Of performed,were
R13516 T17874 T17875 arg1Of performed,according
R13517 T17876 T17875 arg2Of to,according
R13518 T17877 T17878 arg1Of institutional,and
R13519 T17879 T17878 arg2Of state,and
R13520 T17880 T17876 arg2Of guidelines,to
R13521 T17880 T17877 arg1Of guidelines,institutional
R13522 T17880 T17879 arg1Of guidelines,state
R13649 T18081 T18082 arg1Of Isolation,of
R13650 T18085 T18082 arg2Of cells,of
R13651 T18085 T18083 arg1Of cells,CD4+
R13652 T18085 T18084 arg1Of cells,T
R13653 T18088 T18086 arg1Of cells,CD4+
R13654 T18088 T18087 arg1Of cells,T
R13655 T18088 T18089 arg1Of cells,were
R13656 T18088 T18090 arg2Of cells,isolated
R13657 T18090 T18089 arg2Of isolated,were
R13658 T18090 T18091 arg1Of isolated,from
R13659 T18092 T18091 arg2Of blood,from
R13660 T18092 T18093 arg1Of blood,of
R13661 T18096 T18093 arg2Of volunteers,of
R13662 T18096 T18094 arg1Of volunteers,healthy
R13663 T18096 T18095 arg1Of volunteers,human
R13664 T18096 T18097 arg1Of volunteers,using
R13665 T18096 T18109 arg1Of volunteers,as
R13666 T18097 T18102 arg1Of using,(
R13667 T18101 T18097 arg2Of beads,using
R13668 T18101 T18098 arg1Of beads,the
R13669 T18101 T18099 arg1Of beads,anti-CD4
R13670 T18101 T18100 arg1Of beads,magnetic
R13671 T18103 T18104 arg1Of Dynal,","
R13672 T18104 T18102 arg2Of ",",(
R13673 T18104 T18106 arg1Of ",",","
R13674 T18105 T18104 arg2Of Hamburg,","
R13675 T18107 T18106 arg2Of Germany,","
R13676 T18108 T18102 arg3Of ),(
R13677 T18111 T18110 arg1Of described,previously
R13678 T18113 T18109 arg2Of 60,as
R13679 T18113 T18111 arg1Of 60,described
R13680 T18113 T18112 arg2Of 60,[
R13681 T18114 T18112 arg3Of ],[
R13682 T18116 T18115 arg1Of purity,The
R13683 T18116 T18117 arg1Of purity,of
R13684 T18116 T18121 arg1Of purity,was
R13685 T18116 T18123 arg2Of purity,tested
R13686 T18116 T18127 arg1Of purity,was
R13687 T18120 T18117 arg2Of cells,of
R13688 T18120 T18118 arg1Of cells,CD4+
R13689 T18120 T18119 arg1Of cells,T
R13690 T18123 T18121 arg2Of tested,was
R13691 T18123 T18122 arg1Of tested,initially
R13692 T18123 T18126 arg1Of tested,and
R13693 T18125 T18123 arg1Of FACS,tested
R13694 T18125 T18124 arg2Of FACS,by
R13695 T18127 T18126 arg2Of was,and
R13696 T18129 T18128 arg1Of 95,≥
R13697 T18130 T18127 arg2Of %,was
R13698 T18130 T18129 arg1Of %,95
R13699 T18131 T18132 arg1Of Monoclonality,of
R13700 T18131 T18136 arg1Of Monoclonality,was
R13701 T18131 T18137 arg2Of Monoclonality,confirmed
R13702 T18131 T18142 arg1Of Monoclonality,was
R13703 T18131 T18143 arg2Of Monoclonality,identified
R13704 T18131 T18145 arg1Of Monoclonality,be
R13705 T18131 T18147 arg1Of Monoclonality,positive
R13706 T18135 T18132 arg2Of clones,of
R13707 T18135 T18133 arg1Of clones,T
R13708 T18135 T18134 arg1Of clones,cell
R13709 T18137 T18136 arg2Of confirmed,was
R13710 T18137 T18141 arg1Of confirmed,and
R13711 T18140 T18137 arg1Of mapping,confirmed
R13712 T18140 T18138 arg2Of mapping,by
R13713 T18140 T18139 arg1Of mapping,TCR-chain
R13714 T18143 T18141 arg2Of identified,and
R13715 T18143 T18142 arg2Of identified,was
R13716 T18145 T18143 arg3Of be,identified
R13717 T18145 T18144 arg1Of be,to
R13718 T18147 T18145 arg2Of positive,be
R13719 T18147 T18146 arg1Of positive,Vbeta8
R13720 T18149 T18148 arg1Of clones,The
R13721 T18149 T18150 arg1Of clones,were
R13722 T18149 T18151 arg2Of clones,characterized
R13723 T18151 T18150 arg2Of characterized,were
R13724 T18155 T18151 arg1Of secretion,characterized
R13725 T18155 T18152 arg2Of secretion,by
R13726 T18155 T18153 arg1Of secretion,high
R13727 T18155 T18154 arg1Of secretion,IL-4
R14159 T18796 T18793 arg1Of culture,Inducible
R14160 T18796 T18794 arg1Of culture,murine
R14161 T18796 T18795 arg1Of culture,Treg
R14162 T18800 T18797 arg1Of cells,Naive
R14163 T18800 T18798 arg1Of cells,CD4+
R14164 T18800 T18799 arg1Of cells,T
R14165 T18800 T18801 arg1Of cells,(
R14166 T18800 T18809 arg1Of cells,were
R14167 T18800 T18810 arg2Of cells,isolated
R14168 T18802 T18803 arg1Of CD4+,","
R14169 T18803 T18806 arg1Of ",",and
R14170 T18804 T18803 arg2Of CD62L+,","
R14171 T18806 T18801 arg2Of and,(
R14172 T18806 T18805 arg1Of and,","
R14173 T18807 T18806 arg2Of CD25–,and
R14174 T18808 T18801 arg3Of ),(
R14175 T18810 T18809 arg2Of isolated,were
R14176 T18810 T18811 arg1Of isolated,from
R14177 T18814 T18812 arg2Of nodes,pooled
R14178 T18814 T18813 arg1Of nodes,lymph
R14179 T18814 T18815 arg1Of nodes,and
R14180 T18815 T18811 arg2Of and,from
R14181 T18815 T18817 arg1Of and,by
R14182 T18816 T18815 arg2Of spleens,and
R14183 T18818 T18817 arg2Of FACS,by
R14184 T18818 T18819 arg1Of FACS,(
R14185 T18821 T18819 arg2Of Aria,(
R14186 T18821 T18820 arg1Of Aria,FACS
R14187 T18821 T18822 arg1Of Aria,","
R14188 T18824 T18822 arg2Of Biosciences,","
R14189 T18824 T18823 arg1Of Biosciences,BD
R14190 T18825 T18819 arg3Of ),(
R14191 T18828 T18827 arg1Of 105,×
R14192 T18830 T18826 arg1Of cells,5
R14193 T18830 T18828 arg1Of cells,105
R14194 T18830 T18829 arg1Of cells,T
R14195 T18830 T18831 arg1Of cells,were
R14196 T18830 T18832 arg2Of cells,co-cultured
R14197 T18832 T18831 arg2Of co-cultured,were
R14198 T18832 T18833 arg1Of co-cultured,with
R14199 T18832 T18852 arg1Of co-cultured,in
R14200 T18837 T18835 arg1Of bone,×
R14201 T18837 T18836 arg1Of bone,104
R14202 T18838 T18837 arg1Of marrow–derived,bone
R14203 T18840 T18834 arg1Of cells,2.5
R14204 T18840 T18838 arg1Of cells,marrow–derived
R14205 T18840 T18839 arg1Of cells,dendritic
R14206 T18840 T18841 arg1Of cells,[
R14207 T18840 T18844 arg1Of cells,and
R14208 T18842 T18841 arg2Of 62,[
R14209 T18843 T18841 arg3Of ],[
R14210 T18844 T18833 arg2Of and,with
R14211 T18848 T18844 arg2Of peptide,and
R14212 T18848 T18845 arg1Of peptide,0.01
R14213 T18848 T18846 arg1Of peptide,μg/ml
R14214 T18848 T18847 arg1Of peptide,OVA323–339
R14215 T18848 T18849 arg1Of peptide,(
R14216 T18850 T18849 arg2Of Ansynth,(
R14217 T18851 T18849 arg3Of ),(
R14218 T18854 T18855 arg1Of presence,or
R14219 T18855 T18852 arg2Of or,in
R14220 T18855 T18853 arg1Of or,the
R14221 T18855 T18857 arg1Of or,of
R14222 T18856 T18855 arg2Of absence,or
R14223 T18859 T18857 arg2Of rhTGF-ß1,of
R14224 T18859 T18858 arg1Of rhTGF-ß1,20-ng/μl
R14225 T18859 T18860 arg1Of rhTGF-ß1,(
R14226 T18859 T18863 arg1Of rhTGF-ß1,in
R14227 T18861 T18860 arg2Of Peprotech,(
R14228 T18862 T18860 arg3Of ),(
R14229 T18865 T18863 arg2Of plates,in
R14230 T18865 T18864 arg1Of plates,48-well
R14231 T18868 T18866 arg2Of d,After
R14232 T18868 T18867 arg1Of d,4
R14233 T18870 T18871 arg1Of cells,were
R14234 T18870 T18872 arg2Of cells,harvested
R14235 T18870 T18874 arg2Of cells,analyzed
R14236 T18872 T18873 arg1Of harvested,and
R14237 T18873 T18866 arg1Of and,After
R14238 T18873 T18869 arg1Of and,","
R14239 T18873 T18871 arg2Of and,were
R14240 T18874 T18873 arg2Of analyzed,and
R14241 T18874 T18875 arg1Of analyzed,for
R14242 T18878 T18875 arg2Of expression,for
R14243 T18878 T18876 arg1Of expression,intracellular
R14244 T18878 T18877 arg1Of expression,FOXP3
R14245 T18878 T18879 arg1Of expression,by
R14246 T18878 T18884 arg1Of expression,by
R14247 T18880 T18881 arg1Of FACS,or
R14248 T18882 T18881 arg2Of gene,or
R14249 T18883 T18879 arg2Of expression,by
R14250 T18883 T18880 arg1Of expression,FACS
R14251 T18883 T18882 arg1Of expression,gene
R14252 T18886 T18884 arg2Of RT-PCR,by
R14253 T18886 T18885 arg1Of RT-PCR,quantitative
R14371 T19114 T19113 arg1Of vitro,In
R14372 T19117 T19114 arg1Of differentiation,vitro
R14373 T19117 T19115 arg1Of differentiation,T
R14374 T19117 T19116 arg1Of differentiation,cell
R14375 T19120 T19118 arg1Of magnetically-sorted,CD4+
R14376 T19120 T19119 arg1Of magnetically-sorted,CD45RA+
R14377 T19120 T19121 arg1Of magnetically-sorted,(
R14378 T19123 T19121 arg2Of depletion,(
R14379 T19123 T19122 arg1Of depletion,CD45RO
R14380 T19123 T19124 arg1Of depletion,","
R14381 T19123 T19126 arg1Of depletion,","
R14382 T19123 T19127 arg1Of depletion,according
R14383 T19125 T19124 arg2Of MACS,","
R14384 T19128 T19127 arg2Of to,according
R14385 T19130 T19128 arg2Of protocol,to
R14386 T19130 T19129 arg1Of protocol,the
R14387 T19130 T19131 arg1Of protocol,of
R14388 T19133 T19131 arg2Of manufacturer,of
R14389 T19133 T19132 arg1Of manufacturer,the
R14390 T19134 T19121 arg3Of ),(
R14391 T19135 T19120 arg1Of cells,magnetically-sorted
R14392 T19135 T19136 arg1Of cells,were
R14393 T19135 T19137 arg2Of cells,stimulated
R14394 T19137 T19136 arg2Of stimulated,were
R14395 T19137 T19138 arg1Of stimulated,with
R14396 T19137 T19159 arg1Of stimulated,:
R14397 T19141 T19139 arg2Of anti-CD3,immobilized
R14398 T19141 T19140 arg1Of anti-CD3,plate-bound
R14399 T19141 T19142 arg1Of anti-CD3,(
R14400 T19141 T19150 arg1Of anti-CD3,and
R14401 T19144 T19142 arg2Of μg/ml,(
R14402 T19144 T19143 arg1Of μg/ml,1
R14403 T19144 T19145 arg1Of μg/ml,","
R14404 T19144 T19147 arg1Of μg/ml,","
R14405 T19146 T19145 arg2Of Okt3,","
R14406 T19148 T19147 arg2Of IgG1,","
R14407 T19149 T19142 arg3Of ),(
R14408 T19150 T19138 arg2Of and,with
R14409 T19151 T19150 arg2Of anti-CD28,and
R14410 T19151 T19152 arg1Of anti-CD28,(
R14411 T19151 T19156 arg1Of anti-CD28,in
R14412 T19154 T19152 arg2Of μg/ml,(
R14413 T19154 T19153 arg1Of μg/ml,2
R14414 T19155 T19152 arg3Of ),(
R14415 T19158 T19156 arg2Of conditions,in
R14416 T19158 T19157 arg1Of conditions,Th1
R14417 T19162 T19160 arg1Of IL-12,25
R14418 T19162 T19161 arg1Of IL-12,ng/ml
R14419 T19162 T19163 arg1Of IL-12,","
R14420 T19163 T19177 arg1Of ",",:
R14421 T19166 T19164 arg1Of anti-IL-4,5
R14422 T19166 T19165 arg1Of anti-IL-4,μg/ml
R14423 T19166 T19167 arg1Of anti-IL-4,(
R14424 T19166 T19173 arg1Of anti-IL-4,;
R14425 T19171 T19167 arg2Of systems,(
R14426 T19171 T19168 arg1Of systems,R
R14427 T19171 T19169 arg1Of systems,&
R14428 T19171 T19170 arg1Of systems,D
R14429 T19172 T19167 arg3Of ),(
R14430 T19174 T19166 arg1Of in,anti-IL-4
R14431 T19176 T19163 arg2Of conditions,","
R14432 T19176 T19174 arg1Of conditions,in
R14433 T19176 T19175 arg1Of conditions,Th2
R14434 T19180 T19178 arg1Of IL-4,25
R14435 T19180 T19179 arg1Of IL-4,ng/ml
R14436 T19180 T19181 arg1Of IL-4,","
R14437 T19181 T19183 arg1Of ",",μg/ml
R14438 T19181 T19197 arg1Of ",",in
R14439 T19181 T19200 arg1Of ",",:
R14440 T19182 T19181 arg2Of 5,","
R14441 T19183 T19196 arg1Of μg/ml,or
R14442 T19184 T19183 arg2Of anti-IFN-γ,μg/ml
R14443 T19184 T19185 arg1Of anti-IFN-γ,","
R14444 T19188 T19185 arg2Of anti-IL-12,","
R14445 T19188 T19186 arg1Of anti-IL-12,5
R14446 T19188 T19187 arg1Of anti-IL-12,μg/ml
R14447 T19188 T19189 arg1Of anti-IL-12,(
R14448 T19193 T19189 arg2Of systems,(
R14449 T19193 T19190 arg1Of systems,R
R14450 T19193 T19191 arg1Of systems,&
R14451 T19193 T19192 arg1Of systems,D
R14452 T19194 T19189 arg3Of ),(
R14453 T19196 T19195 arg1Of or,;
R14454 T19197 T19196 arg2Of in,or
R14455 T19199 T19197 arg2Of conditions,in
R14456 T19199 T19198 arg1Of conditions,Treg
R14457 T19203 T19201 arg1Of TGF-β,10
R14458 T19203 T19202 arg1Of TGF-β,ng/ml
R14459 T19203 T19204 arg1Of TGF-β,","
R14460 T19203 T19212 arg1Of TGF-β,","
R14461 T19207 T19206 arg1Of anti-IFN-γ,μg/ml
R14462 T19207 T19208 arg1Of anti-IFN-γ,","
R14463 T19209 T19208 arg2Of 5,","
R14464 T19211 T19204 arg2Of anti-IL-12,","
R14465 T19211 T19205 arg1Of anti-IL-12,5
R14466 T19211 T19207 arg1Of anti-IL-12,anti-IFN-γ
R14467 T19211 T19209 arg1Of anti-IL-12,5
R14468 T19211 T19210 arg1Of anti-IL-12,μg/ml
R14469 T19215 T19212 arg2Of anti-IL-4,","
R14470 T19215 T19213 arg1Of anti-IL-4,5
R14471 T19215 T19214 arg1Of anti-IL-4,μg/ml
R14472 T19217 T19216 arg1Of cells,Proliferating
R14473 T19217 T19218 arg1Of cells,were
R14474 T19217 T19219 arg2Of cells,expanded
R14475 T19219 T19218 arg2Of expanded,were
R14476 T19219 T19220 arg1Of expanded,in
R14477 T19221 T19220 arg2Of medium,in
R14478 T19221 T19222 arg1Of medium,containing
R14479 T19223 T19222 arg2Of IL-2,containing
R14480 T19223 T19224 arg1Of IL-2,(
R14481 T19226 T19224 arg2Of ng/ml,(
R14482 T19226 T19225 arg1Of ng/ml,30
R14483 T19227 T19224 arg3Of ),(
R14636 T19534 T19536 arg2Of promoter,cloned
R14637 T19536 T19535 arg2Of cloned,was
R14638 T19536 T19537 arg1Of cloned,into
R14639 T19536 T19546 modOf cloned,to
R14640 T19541 T19537 arg2Of vector,into
R14641 T19541 T19538 arg1Of vector,the
R14642 T19541 T19539 arg1Of vector,pGL3
R14643 T19541 T19540 arg1Of vector,basic
R14644 T19541 T19542 arg1Of vector,(
R14645 T19544 T19542 arg2Of Biotech,(
R14646 T19544 T19543 arg1Of Biotech,Promega
R14647 T19545 T19542 arg3Of ),(
R14648 T19547 T19546 arg1Of generate,to
R14649 T19550 T19547 arg2Of FOXP3,generate
R14650 T19550 T19548 arg1Of FOXP3,the
R14651 T19550 T19549 arg1Of FOXP3,pGL3
R14652 T19550 T19551 arg1Of FOXP3,−511/+176
R14653 T19550 T19552 arg1Of FOXP3,[
R14654 T19553 T19552 arg2Of 24,[
R14655 T19554 T19552 arg3Of ],[
R14656 T19556 T19555 arg1Of mutagenesis,Site-directed
R14657 T19556 T19557 arg1Of mutagenesis,in
R14658 T19556 T19562 arg1Of mutagenesis,were
R14659 T19556 T19563 arg2Of mutagenesis,introduced
R14660 T19561 T19557 arg2Of region,in
R14661 T19561 T19558 arg1Of region,the
R14662 T19561 T19559 arg1Of region,FOXP3
R14663 T19561 T19560 arg1Of region,promoter
R14664 T19563 T19562 arg2Of introduced,were
R14665 T19563 T19564 modOf introduced,using
R14666 T19563 T19571 arg1Of introduced,","
R14667 T19563 T19572 arg1Of introduced,according
R14668 T19567 T19564 arg2Of kit,using
R14669 T19567 T19565 arg1Of kit,the
R14670 T19567 T19566 arg1Of kit,QuickChange
R14671 T19567 T19568 arg1Of kit,(
R14672 T19569 T19568 arg2Of Stratagene,(
R14673 T19570 T19568 arg3Of ),(
R14674 T19573 T19572 arg2Of to,according
R14675 T19575 T19574 arg1Of manufacturer,the
R14676 T19575 T19576 arg2Of manufacturer,'s
R14677 T19577 T19573 arg2Of instructions,to
R14678 T19577 T19576 arg1Of instructions,'s
R14679 T19580 T19578 arg1Of primer,The
R14680 T19580 T19579 arg1Of primer,following
R14681 T19580 T19581 arg1Of primer,and
R14682 T19581 T19585 arg1Of and,were
R14683 T19581 T19586 arg2Of and,used
R14684 T19584 T19581 arg2Of strand,and
R14685 T19584 T19582 arg1Of strand,its
R14686 T19584 T19583 arg1Of strand,complementary
R14687 T19586 T19585 arg2Of used,were
R14688 T19586 T19587 arg1Of used,:
R14689 T19592 T19588 arg1Of CCC,GTT
R14690 T19592 T19589 arg1Of CCC,TCT
R14691 T19592 T19590 arg1Of CCC,CAT
R14692 T19592 T19591 arg1Of CCC,GAG
R14693 T19598 T19592 arg1Of ACC,CCC
R14694 T19598 T19593 arg1Of ACC,TAT
R14695 T19598 T19594 arg1Of ACC,TAA
R14696 T19598 T19595 arg1Of ACC,GTC
R14697 T19598 T19596 arg1Of ACC,ATT
R14698 T19598 T19597 arg1Of ACC,CTT
R14699 T19604 T19586 arg3Of GA,used
R14700 T19604 T19598 arg1Of GA,ACC
R14701 T19604 T19599 arg1Of GA,TCT
R14702 T19604 T19600 arg1Of GA,CAC
R14703 T19604 T19601 arg1Of GA,CTC
R14704 T19604 T19602 arg1Of GA,TGT
R14705 T19604 T19603 arg1Of GA,GGT
R14795 T19770 T19771 arg1Of Transfections,and
R14796 T19774 T19771 arg2Of assays,and
R14797 T19774 T19772 arg1Of assays,reporter
R14798 T19774 T19773 arg1Of assays,gene
R14799 T19776 T19775 arg1Of cells,T
R14800 T19776 T19777 arg1Of cells,were
R14801 T19776 T19778 arg2Of cells,rested
R14802 T19778 T19777 arg2Of rested,were
R14803 T19778 T19779 arg1Of rested,in
R14804 T19778 T19787 arg1Of rested,overnight
R14805 T19782 T19779 arg2Of medium,in
R14806 T19782 T19780 arg1Of medium,serum-free
R14807 T19782 T19781 arg1Of medium,AIM-V
R14808 T19782 T19783 arg1Of medium,(
R14809 T19785 T19783 arg2Of Technologies,(
R14810 T19785 T19784 arg1Of Technologies,Life
R14811 T19786 T19783 arg3Of ),(
R14812 T19789 T19788 arg1Of μg,3.5
R14813 T19789 T19790 arg1Of μg,of
R14814 T19789 T19797 arg1Of μg,and
R14815 T19796 T19790 arg2Of vector,of
R14816 T19796 T19791 arg1Of vector,the
R14817 T19796 T19792 arg1Of vector,FOXP3
R14818 T19796 T19793 arg1Of vector,promoter
R14819 T19796 T19794 arg1Of vector,luciferase
R14820 T19796 T19795 arg1Of vector,reporter
R14821 T19797 T19801 arg1Of and,were
R14822 T19797 T19802 arg2Of and,added
R14823 T19797 T19822 arg2Of and,electroporated
R14824 T19797 T19823 arg1Of and,using
R14825 T19800 T19797 arg2Of phRL-TK,and
R14826 T19800 T19798 arg1Of phRL-TK,0.5
R14827 T19800 T19799 arg1Of phRL-TK,μg
R14828 T19802 T19803 arg1Of added,to
R14829 T19802 T19821 arg1Of added,and
R14830 T19806 T19805 arg1Of 106,×
R14831 T19809 T19803 arg2Of cells,to
R14832 T19809 T19804 arg1Of cells,3
R14833 T19809 T19806 arg1Of cells,106
R14834 T19809 T19807 arg1Of cells,CD4+
R14835 T19809 T19808 arg1Of cells,T
R14836 T19809 T19810 arg2Of cells,resuspended
R14837 T19810 T19811 arg1Of resuspended,in
R14838 T19813 T19811 arg2Of μL,in
R14839 T19813 T19812 arg1Of μL,100
R14840 T19813 T19814 arg1Of μL,of
R14841 T19816 T19814 arg2Of solution,of
R14842 T19816 T19815 arg1Of solution,Nucleofector
R14843 T19816 T19817 arg1Of solution,(
R14844 T19819 T19817 arg2Of Biosystems,(
R14845 T19819 T19818 arg1Of Biosystems,Amaxa
R14846 T19820 T19817 arg3Of ),(
R14847 T19821 T19801 arg2Of and,were
R14848 T19822 T19821 arg2Of electroporated,and
R14849 T19823 T19822 arg3Of using,electroporated
R14850 T19823 T19830 arg1Of using,After
R14851 T19823 T19834 arg1Of using,in
R14852 T19826 T19823 arg2Of program,using
R14853 T19826 T19824 arg1Of program,the
R14854 T19826 T19825 arg1Of program,U-15
R14855 T19826 T19827 arg1Of program,of
R14856 T19829 T19827 arg2Of Nucleofector.,of
R14857 T19829 T19828 arg1Of Nucleofector.,the
R14858 T19833 T19830 arg2Of culture,After
R14859 T19833 T19831 arg1Of culture,a
R14860 T19833 T19832 arg1Of culture,24-h
R14861 T19836 T19837 arg1Of conditions,and
R14862 T19837 T19834 arg2Of and,in
R14863 T19837 T19835 arg1Of and,serum-free
R14864 T19837 T19839 arg1Of and,as
R14865 T19837 T19847 modOf and,was
R14866 T19838 T19837 arg2Of stimuli,and
R14867 T19840 T19839 arg2Of indicated,as
R14868 T19840 T19841 arg1Of indicated,in
R14869 T19843 T19841 arg2Of figures,in
R14870 T19843 T19842 arg1Of figures,the
R14871 T19846 T19845 arg1Of activity,luciferase
R14872 T19846 T19847 arg1Of activity,was
R14873 T19846 T19848 arg2Of activity,measured
R14874 T19848 T19844 arg1Of measured,","
R14875 T19848 T19847 arg2Of measured,was
R14876 T19848 T19849 arg1Of measured,by
R14877 T19848 T19859 arg1Of measured,according
R14878 T19854 T19849 arg2Of system,by
R14879 T19854 T19850 arg1Of system,the
R14880 T19854 T19851 arg1Of system,dual
R14881 T19854 T19852 arg1Of system,luciferase
R14882 T19854 T19853 arg1Of system,assay
R14883 T19854 T19855 arg1Of system,(
R14884 T19857 T19855 arg2Of Biotech,(
R14885 T19857 T19856 arg1Of Biotech,Promega
R14886 T19858 T19855 arg3Of ),(
R14887 T19860 T19859 arg2Of to,according
R14888 T19862 T19861 arg1Of manufacturer,the
R14889 T19862 T19863 arg2Of manufacturer,'s
R14890 T19864 T19860 arg2Of instructions,to
R14891 T19864 T19863 arg1Of instructions,'s
R14892 T19865 T19866 arg1Of Data,were
R14893 T19865 T19867 arg2Of Data,normalized
R14894 T19867 T19866 arg2Of normalized,were
R14895 T19870 T19867 arg1Of activity,normalized
R14896 T19870 T19868 arg2Of activity,by
R14897 T19870 T19869 arg1Of activity,the
R14898 T19870 T19871 arg1Of activity,of
R14899 T19873 T19871 arg2Of luciferase,of
R14900 T19873 T19872 arg1Of luciferase,renilla
R15017 T20058 T20057 arg2Of isolated,was
R15018 T20059 T20058 arg3Of using,isolated
R15019 T20059 T20067 arg1Of using,according
R15020 T20063 T20059 arg2Of Kit,using
R15021 T20063 T20060 arg1Of Kit,the
R15022 T20063 T20061 arg1Of Kit,RNeasy
R15023 T20063 T20062 arg1Of Kit,Mini
R15024 T20063 T20064 arg1Of Kit,(
R15025 T20065 T20064 arg2Of Qiagen,(
R15026 T20066 T20064 arg3Of ),(
R15027 T20068 T20067 arg2Of to,according
R15028 T20070 T20069 arg1Of manufacturer,the
R15029 T20070 T20071 arg2Of manufacturer,'s
R15030 T20072 T20068 arg2Of protocol,to
R15031 T20072 T20071 arg1Of protocol,'s
R15032 T20074 T20073 arg1Of transcription,Reverse
R15033 T20074 T20075 arg1Of transcription,of
R15034 T20074 T20078 arg1Of transcription,was
R15035 T20074 T20079 arg2Of transcription,performed
R15036 T20077 T20075 arg2Of samples,of
R15037 T20077 T20076 arg1Of samples,human
R15038 T20079 T20078 arg2Of performed,was
R15039 T20079 T20080 arg1Of performed,with
R15040 T20079 T20091 arg1Of performed,according
R15041 T20083 T20080 arg2Of reagents,with
R15042 T20083 T20081 arg1Of reagents,TaqMan
R15043 T20083 T20082 arg1Of reagents,reverse-transcription
R15044 T20083 T20084 arg1Of reagents,(
R15045 T20083 T20088 arg1Of reagents,with
R15046 T20086 T20084 arg2Of Biosystems,(
R15047 T20086 T20085 arg1Of Biosystems,Applied
R15048 T20087 T20084 arg3Of ),(
R15049 T20090 T20088 arg2Of hexamers,with
R15050 T20090 T20089 arg1Of hexamers,random
R15051 T20092 T20091 arg2Of to,according
R15052 T20094 T20093 arg1Of manufacturer,the
R15053 T20094 T20095 arg2Of manufacturer,'s
R15054 T20096 T20092 arg2Of protocol,to
R15055 T20096 T20095 arg1Of protocol,'s
R15111 T20177 T20175 arg1Of proteins,Recombinant
R15112 T20177 T20176 arg1Of proteins,TAT
R15113 T20179 T20178 arg1Of cDNAs,The
R15114 T20179 T20180 arg1Of cDNAs,encoding
R15115 T20179 T20187 arg1Of cDNAs,(
R15116 T20179 T20194 arg1Of cDNAs,were
R15117 T20179 T20195 arg2Of cDNAs,cloned
R15118 T20182 T20181 arg1Of protein,GATA3
R15119 T20182 T20183 arg1Of protein,or
R15120 T20183 T20180 arg2Of or,encoding
R15121 T20186 T20183 arg2Of GATA3,or
R15122 T20186 T20184 arg1Of GATA3,the
R15123 T20186 T20185 arg2Of GATA3,truncated
R15124 T20188 T20187 arg2Of lacking,(
R15125 T20192 T20188 arg2Of fingers,lacking
R15126 T20192 T20189 arg1Of fingers,the
R15127 T20192 T20190 arg1Of fingers,two
R15128 T20192 T20191 arg1Of fingers,zinc
R15129 T20193 T20187 arg3Of ),(
R15130 T20195 T20194 arg2Of cloned,were
R15131 T20195 T20196 arg1Of cloned,in
R15132 T20195 T20198 arg1Of cloned,into
R15133 T20195 T20203 arg1Of cloned,with
R15134 T20197 T20196 arg2Of frame,in
R15135 T20201 T20198 arg2Of vector,into
R15136 T20201 T20199 arg1Of vector,an
R15137 T20201 T20200 arg1Of vector,expression
R15138 T20203 T20202 arg1Of with,along
R15139 T20206 T20203 arg2Of sequence,with
R15140 T20206 T20204 arg1Of sequence,the
R15141 T20206 T20205 arg1Of sequence,TAT
R15142 T20206 T20207 arg1Of sequence,as
R15143 T20209 T20208 arg1Of described,previously
R15144 T20211 T20207 arg2Of 63,as
R15145 T20211 T20209 arg1Of 63,described
R15146 T20211 T20210 arg2Of 63,[
R15147 T20212 T20210 arg3Of ],[
R15148 T20213 T20214 arg1Of Proteins,were
R15149 T20213 T20215 arg2Of Proteins,expressed
R15150 T20215 T20214 arg2Of expressed,were
R15151 T20215 T20216 arg1Of expressed,in
R15152 T20215 T20226 arg1Of expressed,and
R15153 T20218 T20217 arg1Of Star,BL21
R15154 T20218 T20219 arg1Of Star,(
R15155 T20220 T20219 arg2Of DE3,(
R15156 T20221 T20219 arg3Of ),(
R15157 T20222 T20216 arg2Of pLysS,in
R15158 T20222 T20218 arg1Of pLysS,Star
R15159 T20222 T20223 arg1Of pLysS,(
R15160 T20224 T20223 arg2Of Invitrogen,(
R15161 T20225 T20223 arg3Of ),(
R15162 T20227 T20228 arg1Of lysates,were
R15163 T20227 T20229 arg2Of lysates,purified
R15164 T20229 T20226 arg2Of purified,and
R15165 T20229 T20228 arg2Of purified,were
R15166 T20233 T20229 arg1Of chromatography,purified
R15167 T20233 T20230 arg2Of chromatography,by
R15168 T20233 T20231 arg1Of chromatography,Ni2+-chelate
R15169 T20233 T20232 arg1Of chromatography,column
R15170 T20236 T20234 arg1Of proteins,Both
R15171 T20236 T20235 arg1Of proteins,TAT-linked
R15172 T20236 T20237 arg1Of proteins,were
R15173 T20237 T20243 arg1Of were,","
R15174 T20237 T20244 arg1Of were,based
R15175 T20238 T20239 arg1Of more,than
R15176 T20240 T20239 arg2Of 95,than
R15177 T20240 T20241 arg1Of 95,%
R15178 T20242 T20237 arg2Of pure,were
R15179 T20242 T20238 arg1Of pure,more
R15180 T20245 T20244 arg2Of on,based
R15181 T20248 T20245 arg2Of staining,on
R15182 T20248 T20246 arg1Of staining,Coomassie
R15183 T20248 T20247 arg1Of staining,blue
R15184 T20248 T20249 arg1Of staining,of
R15185 T20253 T20249 arg2Of gels,of
R15186 T20253 T20250 arg1Of gels,sodium
R15187 T20253 T20251 arg1Of gels,disulfate
R15188 T20253 T20252 arg1Of gels,acrylamide
R15291 T20446 T20445 arg1Of transduction,TAT-GATA3
R15292 T20448 T20447 arg1Of cells,CD4+CD45RA+
R15293 T20448 T20449 arg1Of cells,were
R15294 T20448 T20450 arg2Of cells,cultured
R15295 T20448 T20455 arg2Of cells,transduced
R15296 T20450 T20451 arg1Of cultured,in
R15297 T20450 T20454 arg1Of cultured,and
R15298 T20453 T20451 arg2Of medium,in
R15299 T20453 T20452 arg1Of medium,AIMV
R15300 T20454 T20449 arg2Of and,were
R15301 T20455 T20454 arg2Of transduced,and
R15302 T20455 T20456 arg1Of transduced,with
R15303 T20455 T20471 arg1Of transduced,over
R15304 T20458 T20457 arg1Of nM,20
R15305 T20458 T20459 arg1Of nM,","
R15306 T20459 T20463 arg1Of ",",or
R15307 T20461 T20459 arg2Of nM,","
R15308 T20461 T20460 arg1Of nM,10
R15309 T20463 T20456 arg2Of or,with
R15310 T20463 T20462 arg1Of or,","
R15311 T20465 T20463 arg2Of nM,or
R15312 T20465 T20464 arg1Of nM,500
R15313 T20465 T20466 arg1Of nM,of
R15314 T20467 T20468 arg1Of full-length,or
R15315 T20469 T20468 arg2Of truncated,or
R15316 T20470 T20466 arg2Of GATA3,of
R15317 T20470 T20467 arg1Of GATA3,full-length
R15318 T20470 T20469 arg2Of GATA3,truncated
R15319 T20473 T20471 arg2Of course,over
R15320 T20473 T20472 arg1Of course,the
R15321 T20473 T20474 arg1Of course,of
R15322 T20476 T20474 arg2Of h,of
R15323 T20476 T20475 arg1Of h,4
R15324 T20479 T20477 arg2Of h,After
R15325 T20479 T20478 arg1Of h,4
R15326 T20482 T20481 arg1Of cells,the
R15327 T20482 T20483 arg1Of cells,were
R15328 T20482 T20484 arg2Of cells,washed
R15329 T20482 T20486 arg2Of cells,activated
R15330 T20484 T20485 arg1Of washed,and
R15331 T20485 T20477 arg1Of and,After
R15332 T20485 T20480 arg1Of and,","
R15333 T20485 T20483 arg2Of and,were
R15334 T20486 T20485 arg2Of activated,and
R15335 T20486 T20487 arg1Of activated,with
R15336 T20489 T20490 arg1Of anti-CD3,and
R15337 T20490 T20488 arg1Of and,soluble
R15338 T20490 T20492 arg1Of and,and
R15339 T20491 T20490 arg2Of anti-CD28,and
R15340 T20492 T20487 arg2Of and,with
R15341 T20493 T20492 arg2Of TGF-β,and
R15342 T20493 T20494 arg1Of TGF-β,(
R15343 T20496 T20494 arg2Of ng/ml,(
R15344 T20496 T20495 arg1Of ng/ml,10
R15345 T20497 T20494 arg3Of ),(
R15346 T20499 T20498 arg1Of day,Each
R15347 T20503 T20501 arg1Of proteins,the
R15348 T20503 T20502 arg1Of proteins,TAT
R15349 T20503 T20504 arg1Of proteins,were
R15350 T20503 T20506 arg2Of proteins,added
R15351 T20506 T20499 arg1Of added,day
R15352 T20506 T20500 arg1Of added,","
R15353 T20506 T20504 arg2Of added,were
R15354 T20506 T20505 arg1Of added,freshly
R15355 T20506 T20507 arg1Of added,to
R15356 T20509 T20507 arg2Of medium,to
R15357 T20509 T20508 arg1Of medium,the
R15358 T20511 T20510 arg1Of expression,FOXP3
R15359 T20511 T20512 arg1Of expression,was
R15360 T20511 T20513 arg2Of expression,measured
R15361 T20513 T20512 arg2Of measured,was
R15362 T20513 T20514 arg1Of measured,after
R15363 T20516 T20514 arg2Of d,after
R15364 T20516 T20515 arg1Of d,5
R15365 T20516 T20517 arg1Of d,by
R15366 T20519 T20517 arg2Of staining,by
R15367 T20519 T20518 arg1Of staining,intracellular
R15458 T20717 T20715 arg1Of staining,Intracellular
R15459 T20717 T20716 arg1Of staining,cytokine
R15460 T20719 T20718 arg1Of cells,T
R15461 T20719 T20720 arg1Of cells,were
R15462 T20719 T20721 arg2Of cells,stimulated
R15463 T20721 T20720 arg2Of stimulated,were
R15464 T20721 T20722 arg1Of stimulated,with
R15465 T20725 T20723 arg1Of 10−7,2
R15466 T20725 T20724 arg1Of 10−7,×
R15467 T20725 T20726 arg1Of 10−7,M
R15468 T20727 T20725 arg1Of PMA,10−7
R15469 T20727 T20728 arg1Of PMA,and
R15470 T20728 T20722 arg2Of and,with
R15471 T20730 T20728 arg2Of μg/ml,and
R15472 T20730 T20729 arg1Of μg/ml,1
R15473 T20730 T20731 arg1Of μg/ml,of
R15474 T20732 T20731 arg2Of ionomycin,of
R15475 T20732 T20733 arg1Of ionomycin,(
R15476 T20732 T20737 arg1Of ionomycin,for
R15477 T20735 T20733 arg2Of Chemicals,(
R15478 T20735 T20734 arg1Of Chemicals,Sigma
R15479 T20736 T20733 arg3Of ),(
R15480 T20739 T20737 arg2Of h,for
R15481 T20739 T20738 arg1Of h,4
R15482 T20742 T20740 arg1Of mAb,The
R15483 T20742 T20741 arg1Of mAb,following
R15484 T20742 T20743 arg1Of mAb,was
R15485 T20742 T20744 arg2Of mAb,used
R15486 T20744 T20743 arg2Of used,was
R15487 T20744 T20745 arg1Of used,:
R15488 T20744 T20746 arg1Of used,anti-IL-4-PE
R15489 T20746 T20747 arg1Of anti-IL-4-PE,(
R15490 T20748 T20747 arg2Of 8D4–8,(
R15491 T20748 T20749 arg1Of 8D4–8,","
R15492 T20750 T20749 arg2Of BD,","
R15493 T20751 T20747 arg3Of ),(
R15494 T20754 T20752 arg2Of controls,Matched
R15495 T20754 T20753 arg1Of controls,isotype
R15496 T20754 T20755 arg1Of controls,were
R15497 T20754 T20756 arg2Of controls,used
R15498 T20756 T20755 arg2Of used,were
R15499 T20756 T20757 arg1Of used,at
R15500 T20756 T20762 arg1Of used,as
R15501 T20761 T20757 arg2Of concentration,at
R15502 T20761 T20758 arg1Of concentration,the
R15503 T20761 T20759 arg1Of concentration,same
R15504 T20761 T20760 arg1Of concentration,protein
R15505 T20765 T20762 arg2Of antibodies,as
R15506 T20765 T20763 arg1Of antibodies,the
R15507 T20765 T20764 arg1Of antibodies,respective
R15508 T20767 T20766 arg1Of FACS,Four-color
R15509 T20767 T20768 arg1Of FACS,was
R15510 T20767 T20769 arg2Of FACS,performed
R15511 T20769 T20768 arg2Of performed,was
R15512 T20770 T20769 arg3Of using,performed
R15513 T20773 T20770 arg2Of XL-MCL,using
R15514 T20773 T20771 arg1Of XL-MCL,an
R15515 T20773 T20772 arg1Of XL-MCL,EPICS
R15516 T20773 T20774 arg1Of XL-MCL,(
R15517 T20773 T20778 arg1Of XL-MCL,using
R15518 T20776 T20774 arg2Of Coulter,(
R15519 T20776 T20775 arg1Of Coulter,Beckman
R15520 T20777 T20774 arg3Of ),(
R15521 T20782 T20778 arg2Of version,using
R15522 T20782 T20779 arg1Of version,the
R15523 T20782 T20780 arg1Of version,software
R15524 T20782 T20781 arg1Of version,Expo32
R15525 T20782 T20783 arg1Of version,for
R15526 T20785 T20786 arg1Of acquisition,and
R15527 T20786 T20783 arg2Of and,for
R15528 T20786 T20784 arg1Of and,data
R15529 T20787 T20786 arg2Of evaluation,and
R15619 T20969 T20968 arg1Of cytometry,Flow
R15620 T20971 T20970 arg2Of analysis,For
R15621 T20971 T20972 arg1Of analysis,of
R15622 T20974 T20972 arg2Of expression,of
R15623 T20974 T20973 arg1Of expression,FOXP3
R15624 T20974 T20975 arg1Of expression,at
R15625 T20978 T20975 arg2Of level,at
R15626 T20978 T20976 arg1Of level,the
R15627 T20978 T20977 arg1Of level,single-cell
R15628 T20980 T20981 arg1Of cells,were
R15629 T20980 T20983 arg2Of cells,stained
R15630 T20983 T20981 arg2Of stained,were
R15631 T20983 T20982 arg1Of stained,first
R15632 T20983 T20984 arg1Of stained,with
R15633 T20983 T20994 arg1Of stained,and
R15634 T20988 T20984 arg2Of CD25,with
R15635 T20988 T20985 arg1Of CD25,the
R15636 T20988 T20986 arg1Of CD25,monoclonal
R15637 T20988 T20987 arg1Of CD25,antibody
R15638 T20988 T20989 arg1Of CD25,(
R15639 T20991 T20989 arg2Of Coulter,(
R15640 T20991 T20990 arg1Of Coulter,Beckman
R15641 T20992 T20989 arg3Of ),(
R15642 T20994 T20970 arg1Of and,For
R15643 T20994 T20979 arg1Of and,","
R15644 T20994 T20993 arg1Of and,","
R15645 T20996 T20997 arg1Of fixation,and
R15646 T20997 T20995 arg2Of and,after
R15647 T20998 T20997 arg2Of permeabilization,and
R15648 T21000 T21001 arg1Of cells,were
R15649 T21000 T21002 arg2Of cells,incubated
R15650 T21000 T21021 arg2Of cells,subjected
R15651 T21002 T21003 arg1Of incubated,with
R15652 T21002 T21014 arg1Of incubated,based
R15653 T21002 T21020 arg1Of incubated,and
R15654 T21007 T21003 arg2Of PCH101,with
R15655 T21007 T21004 arg1Of PCH101,PE-conjugated
R15656 T21007 T21005 arg1Of PCH101,monoclonal
R15657 T21007 T21006 arg1Of PCH101,antibody
R15658 T21007 T21008 arg1Of PCH101,(
R15659 T21010 T21008 arg2Of FOXP3,(
R15660 T21010 T21009 arg1Of FOXP3,anti-human
R15661 T21010 T21011 arg1Of FOXP3,;
R15662 T21012 T21011 arg2Of eBioscience,;
R15663 T21013 T21008 arg3Of ),(
R15664 T21015 T21014 arg2Of on,based
R15665 T21017 T21016 arg1Of manufacturer,the
R15666 T21017 T21018 arg2Of manufacturer,'s
R15667 T21019 T21015 arg2Of recommendations,on
R15668 T21019 T21018 arg1Of recommendations,'s
R15669 T21020 T20994 arg2Of and,and
R15670 T21020 T20995 arg1Of and,after
R15671 T21020 T20999 arg1Of and,","
R15672 T21020 T21001 arg2Of and,were
R15673 T21021 T21020 arg2Of subjected,and
R15674 T21021 T21022 arg1Of subjected,to
R15675 T21023 T21022 arg2Of FACS,to
R15676 T21023 T21024 arg1Of FACS,(
R15677 T21026 T21024 arg2Of XL-MCL,(
R15678 T21026 T21025 arg1Of XL-MCL,EPICS
R15679 T21027 T21024 arg3Of ),(
R15680 T21032 T21028 arg2Of staining,For
R15681 T21032 T21029 arg1Of staining,cell
R15682 T21032 T21030 arg1Of staining,surface
R15683 T21032 T21031 arg1Of staining,marker
R15684 T21034 T21035 arg1Of cells,were
R15685 T21034 T21036 arg2Of cells,incubated
R15686 T21036 T21028 arg1Of incubated,For
R15687 T21036 T21033 arg1Of incubated,","
R15688 T21036 T21035 arg2Of incubated,were
R15689 T21036 T21037 arg1Of incubated,for
R15690 T21036 T21040 arg1Of incubated,at
R15691 T21036 T21045 arg1Of incubated,with
R15692 T21039 T21037 arg2Of min,for
R15693 T21039 T21038 arg1Of min,20
R15694 T21041 T21040 arg2Of 4°C,at
R15695 T21041 T21042 arg1Of 4°C,in
R15696 T21044 T21042 arg2Of buffer,in
R15697 T21044 T21043 arg1Of buffer,staining
R15698 T21048 T21045 arg2Of antibodies,with
R15699 T21048 T21046 arg1Of antibodies,the
R15700 T21048 T21047 arg1Of antibodies,following
R15701 T21048 T21049 arg1Of antibodies,:
R15702 T21050 T21051 arg1Of anti–CD152-PE,(
R15703 T21050 T21056 arg1Of anti–CD152-PE,","
R15704 T21052 T21051 arg2Of CTLA-4,(
R15705 T21052 T21053 arg1Of CTLA-4,;
R15706 T21054 T21053 arg2Of BD,;
R15707 T21055 T21051 arg3Of ),(
R15708 T21056 T21049 arg2Of ",",:
R15709 T21056 T21061 arg1Of ",",","
R15710 T21056 T21069 arg1Of ",",","
R15711 T21057 T21056 arg2Of anti–PD-1,","
R15712 T21057 T21058 arg1Of anti–PD-1,(
R15713 T21059 T21058 arg2Of eBiosciences,(
R15714 T21060 T21058 arg3Of ),(
R15715 T21062 T21061 arg2Of anti-GITR,","
R15716 T21062 T21063 arg1Of anti-GITR,(
R15717 T21067 T21063 arg2Of Systems,(
R15718 T21067 T21064 arg1Of Systems,R
R15719 T21067 T21065 arg1Of Systems,&
R15720 T21067 T21066 arg1Of Systems,D
R15721 T21068 T21063 arg3Of ),(
R15722 T21070 T21071 arg1Of anti-CD69,(
R15723 T21070 T21075 arg1Of anti-CD69,","
R15724 T21073 T21071 arg2Of Coulter,(
R15725 T21073 T21072 arg1Of Coulter,Beckman
R15726 T21074 T21071 arg3Of ),(
R15727 T21075 T21080 arg1Of ",",","
R15728 T21076 T21075 arg2Of anti-CD103,","
R15729 T21076 T21077 arg1Of anti-CD103,(
R15730 T21078 T21077 arg2Of DakoCytomation,(
R15731 T21079 T21077 arg3Of ),(
R15732 T21080 T21087 arg1Of ",",or
R15733 T21081 T21080 arg2Of anti-CD62L,","
R15734 T21081 T21082 arg1Of anti-CD62L,(
R15735 T21084 T21082 arg2Of Coulter,(
R15736 T21084 T21083 arg1Of Coulter,Beckman
R15737 T21085 T21082 arg3Of ),(
R15738 T21087 T21069 arg2Of or,","
R15739 T21087 T21086 arg1Of or,","
R15740 T21088 T21087 arg2Of anti-HLA-DR,or
R15741 T21088 T21089 arg1Of anti-HLA-DR,(
R15742 T21091 T21089 arg2Of Coulter,(
R15743 T21091 T21090 arg1Of Coulter,Beckman
R15744 T21092 T21089 arg3Of ),(
R15745 T21094 T21093 arg1Of controls,The
R15746 T21094 T21095 arg1Of controls,were
R15747 T21096 T21097 arg1Of FITC,","
R15748 T21097 T21100 arg1Of ",",or
R15749 T21098 T21097 arg2Of PE,","
R15750 T21100 T21095 arg2Of or,were
R15751 T21100 T21099 arg1Of or,","
R15752 T21103 T21101 arg1Of IgG1,ECD-conjugated
R15753 T21103 T21102 arg1Of IgG1,mouse
R15754 T21103 T21104 arg1Of IgG1,or
R15755 T21104 T21100 arg2Of or,or
R15756 T21106 T21104 arg2Of IgG2a,or
R15757 T21106 T21105 arg1Of IgG2a,rat
R15758 T21108 T21107 arg2Of staining,For
R15759 T21108 T21109 arg1Of staining,of
R15760 T21111 T21109 arg2Of cells,of
R15761 T21111 T21110 arg1Of cells,mouse
R15762 T21115 T21113 arg1Of mAbs,the
R15763 T21115 T21114 arg1Of mAbs,following
R15764 T21115 T21116 arg1Of mAbs,from
R15765 T21115 T21119 arg1Of mAbs,were
R15766 T21115 T21120 arg2Of mAbs,used
R15767 T21118 T21116 arg2Of Biosciences,from
R15768 T21118 T21117 arg1Of Biosciences,BD
R15769 T21120 T21107 arg1Of used,For
R15770 T21120 T21112 arg1Of used,","
R15771 T21120 T21119 arg2Of used,were
R15772 T21120 T21121 arg1Of used,following
R15773 T21120 T21124 arg1Of used,as
R15774 T21123 T21121 arg2Of techniques,following
R15775 T21123 T21122 arg1Of techniques,standard
R15776 T21125 T21124 arg2Of described,as
R15777 T21125 T21126 arg1Of described,above
R15778 T21126 T21127 arg1Of above,:
R15779 T21128 T21129 arg1Of anti-CD3,","
R15780 T21129 T21132 arg1Of ",",and
R15781 T21130 T21129 arg2Of anti-CD4,","
R15782 T21132 T21126 arg2Of and,above
R15783 T21132 T21131 arg1Of and,","
R15784 T21133 T21132 arg2Of anti-CD25,and
R15785 T21135 T21134 arg1Of antibody,Anti-FcγRII/III
R15786 T21135 T21136 arg1Of antibody,(
R15787 T21135 T21141 arg1Of antibody,was
R15788 T21135 T21142 arg2Of antibody,included
R15789 T21135 T21147 arg1Of antibody,reduce
R15790 T21137 T21136 arg2Of 2.4G2,(
R15791 T21137 T21138 arg1Of 2.4G2,","
R15792 T21139 T21138 arg2Of ATCC,","
R15793 T21140 T21136 arg3Of ),(
R15794 T21142 T21141 arg2Of included,was
R15795 T21142 T21143 arg1Of included,in
R15796 T21142 T21146 modOf included,to
R15797 T21145 T21143 arg2Of stainings,in
R15798 T21145 T21144 arg1Of stainings,all
R15799 T21147 T21146 arg1Of reduce,to
R15800 T21150 T21147 arg2Of binding,reduce
R15801 T21150 T21148 arg1Of binding,nonspecific
R15802 T21150 T21149 arg1Of binding,antibody
R15803 T21152 T21151 arg1Of isolate,To
R15804 T21157 T21152 arg2Of cells,isolate
R15805 T21157 T21153 arg1Of cells,naive
R15806 T21157 T21154 arg1Of cells,murine
R15807 T21157 T21155 arg1Of cells,CD4
R15808 T21157 T21156 arg1Of cells,T
R15809 T21157 T21158 arg1Of cells,from
R15810 T21160 T21159 arg1Of DO11.10,murine
R15811 T21160 T21161 arg1Of DO11.10,or
R15812 T21161 T21158 arg2Of or,from
R15813 T21164 T21161 arg2Of cells,or
R15814 T21164 T21162 arg1Of cells,DO11.10xCD2-GATA3
R15815 T21164 T21163 arg1Of cells,T
R15816 T21166 T21152 arg1Of cells,isolate
R15817 T21166 T21167 arg1Of cells,were
R15818 T21166 T21168 arg2Of cells,stained
R15819 T21168 T21151 modOf stained,To
R15820 T21168 T21165 arg1Of stained,","
R15821 T21168 T21167 arg2Of stained,were
R15822 T21168 T21169 arg1Of stained,with
R15823 T21170 T21171 arg1Of anti-CD25-FITC,","
R15824 T21171 T21174 arg1Of ",",and
R15825 T21172 T21171 arg2Of anti-CD62L-PE,","
R15826 T21174 T21169 arg2Of and,with
R15827 T21174 T21173 arg1Of and,","
R15828 T21174 T21176 arg1Of and,prior
R15829 T21175 T21174 arg2Of anti-CD4-APC,and
R15830 T21176 T21177 arg1Of prior,to
R15831 T21178 T21177 arg2Of sorting,to
R15832 T21180 T21179 arg1Of cells,Dead
R15833 T21180 T21181 arg1Of cells,were
R15834 T21180 T21182 arg2Of cells,excluded
R15835 T21182 T21181 arg2Of excluded,were
R15836 T21182 T21183 arg1Of excluded,with
R15837 T21184 T21183 arg2Of 4′,with
R15838 T21184 T21185 arg1Of 4′,'
R15839 T21184 T21186 arg1Of 4′,","
R15840 T21187 T21186 arg2Of 6-Diamidino-2-phenylindole,","
R15841 T21187 T21188 arg1Of 6-Diamidino-2-phenylindole,(
R15842 T21189 T21188 arg2Of DAPI,(
R15843 T21190 T21188 arg3Of ),(
R15844 T21192 T21191 arg1Of analyze,To
R15845 T21195 T21192 arg2Of expression,analyze
R15846 T21195 T21193 arg1Of expression,murine
R15847 T21195 T21194 arg1Of expression,Foxp3
R15848 T21195 T21196 arg1Of expression,in
R15849 T21199 T21196 arg2Of cultures,in
R15850 T21199 T21197 arg1Of cultures,inducible
R15851 T21199 T21198 arg1Of cultures,Treg
R15852 T21201 T21192 arg1Of cells,analyze
R15853 T21201 T21202 arg1Of cells,were
R15854 T21201 T21203 arg2Of cells,stained
R15855 T21203 T21191 modOf stained,To
R15856 T21203 T21200 arg1Of stained,","
R15857 T21203 T21202 arg2Of stained,were
R15858 T21203 T21204 arg1Of stained,intracellularly
R15859 T21203 T21205 arg1Of stained,with
R15860 T21203 T21207 arg1Of stained,according
R15861 T21203 T21212 arg1Of stained,","
R15862 T21203 T21213 arg1Of stained,in
R15863 T21206 T21205 arg2Of anti-Foxp3-PE,with
R15864 T21208 T21207 arg2Of to,according
R15865 T21209 T21210 arg2Of manufacturer,'s
R15866 T21211 T21208 arg2Of instruction,to
R15867 T21211 T21210 arg1Of instruction,'s
R15868 T21213 T21215 arg1Of in,with
R15869 T21214 T21213 arg2Of conjunction,in
R15870 T21216 T21217 arg1Of anti-CD4-APC,and
R15871 T21218 T21217 arg2Of LIVE/DEAD,and
R15872 T21223 T21213 arg3Of kit,in
R15873 T21223 T21216 arg1Of kit,anti-CD4-APC
R15874 T21223 T21218 arg1Of kit,LIVE/DEAD
R15875 T21223 T21219 arg1Of kit,fixable
R15876 T21223 T21220 arg1Of kit,dead
R15877 T21223 T21221 arg1Of kit,cell
R15878 T21223 T21222 arg1Of kit,stain
R15879 T21223 T21224 arg1Of kit,(
R15880 T21223 T21227 modOf kit,to
R15881 T21223 T21228 arg1Of kit,discriminate
R15882 T21225 T21224 arg2Of Invitrogen,(
R15883 T21226 T21224 arg3Of ),(
R15884 T21228 T21227 arg1Of discriminate,to
R15885 T21230 T21228 arg2Of cells,discriminate
R15886 T21230 T21229 arg1Of cells,live
R15887 T21233 T21231 arg1Of antibodies,All
R15888 T21233 T21232 arg1Of antibodies,monoclonal
R15889 T21233 T21234 arg1Of antibodies,for
R15890 T21233 T21238 arg1Of antibodies,were
R15891 T21233 T21239 arg2Of antibodies,purchased
R15892 T21237 T21234 arg2Of stainings,for
R15893 T21237 T21235 arg1Of stainings,murine
R15894 T21237 T21236 arg1Of stainings,cell
R15895 T21239 T21238 arg2Of purchased,were
R15896 T21239 T21240 arg1Of purchased,from
R15897 T21241 T21242 arg1Of eBioscience,or
R15898 T21242 T21240 arg2Of or,from
R15899 T21244 T21242 arg2Of Biosciences,or
R15900 T21244 T21243 arg1Of Biosciences,BD
R16212 T21791 T21790 arg1Of blotting,Western
R16213 T21794 T21792 arg2Of analysis,For
R16214 T21794 T21793 arg1Of analysis,FOXP3
R16215 T21794 T21795 arg1Of analysis,on
R16216 T21798 T21795 arg2Of level,on
R16217 T21798 T21796 arg1Of level,the
R16218 T21798 T21797 arg1Of level,protein
R16219 T21800 T21801 arg1Of 1,×
R16220 T21802 T21801 arg2Of 106,×
R16221 T21804 T21800 arg1Of cells,1
R16222 T21804 T21802 arg1Of cells,106
R16223 T21804 T21803 arg1Of cells,CD4+CD25–
R16224 T21804 T21805 arg1Of cells,were
R16225 T21804 T21806 arg2Of cells,lysed
R16226 T21804 T21808 arg2Of cells,loaded
R16227 T21806 T21807 arg1Of lysed,and
R16228 T21807 T21792 arg1Of and,For
R16229 T21807 T21799 arg1Of and,","
R16230 T21807 T21805 arg2Of and,were
R16231 T21807 T21819 arg1Of and,on
R16232 T21808 T21807 arg2Of loaded,and
R16233 T21808 T21810 arg1Of loaded,to
R16234 T21808 T21814 arg1Of loaded,(
R16235 T21810 T21809 arg1Of to,next
R16236 T21813 T21810 arg2Of ladder,to
R16237 T21813 T21811 arg1Of ladder,a
R16238 T21813 T21812 arg1Of ladder,protein-mass
R16239 T21815 T21814 arg2Of Magicmark,(
R16240 T21815 T21816 arg1Of Magicmark,","
R16241 T21815 T21817 arg1Of Magicmark,Invitrogen
R16242 T21818 T21814 arg3Of ),(
R16243 T21822 T21823 arg1Of 4–12,%
R16244 T21825 T21819 arg2Of gel,on
R16245 T21825 T21820 arg1Of gel,a
R16246 T21825 T21821 arg1Of gel,NuPAGE
R16247 T21825 T21822 arg1Of gel,4–12
R16248 T21825 T21824 arg1Of gel,bis-tris
R16249 T21825 T21826 arg1Of gel,(
R16250 T21827 T21826 arg2Of Invitrogen,(
R16251 T21828 T21826 arg3Of ),(
R16252 T21830 T21829 arg1Of proteins,The
R16253 T21830 T21831 arg1Of proteins,were
R16254 T21830 T21832 arg2Of proteins,electroblotted
R16255 T21832 T21831 arg2Of electroblotted,were
R16256 T21832 T21833 arg1Of electroblotted,onto
R16257 T21832 T21837 arg1Of electroblotted,(
R16258 T21836 T21833 arg2Of membrane,onto
R16259 T21836 T21834 arg1Of membrane,a
R16260 T21836 T21835 arg1Of membrane,PVDF
R16261 T21840 T21837 arg2Of Science,(
R16262 T21840 T21838 arg1Of Science,Amersham
R16263 T21840 T21839 arg1Of Science,Life
R16264 T21841 T21837 arg3Of ),(
R16265 T21843 T21842 arg1Of binding,Unspecific
R16266 T21843 T21844 arg1Of binding,was
R16267 T21843 T21845 arg2Of binding,blocked
R16268 T21845 T21844 arg2Of blocked,was
R16269 T21845 T21846 arg1Of blocked,with
R16270 T21845 T21849 arg1Of blocked,and
R16271 T21847 T21846 arg2Of BSA,with
R16272 T21849 T21848 arg1Of and,","
R16273 T21849 T21858 arg1Of and,:
R16274 T21851 T21850 arg1Of membranes,the
R16275 T21851 T21852 arg1Of membranes,were
R16276 T21851 T21854 arg2Of membranes,incubated
R16277 T21854 T21849 arg2Of incubated,and
R16278 T21854 T21852 arg2Of incubated,were
R16279 T21854 T21853 arg1Of incubated,subsequently
R16280 T21854 T21855 arg1Of incubated,with
R16281 T21857 T21855 arg2Of 1,with
R16282 T21857 T21856 arg1Of 1,an
R16283 T21860 T21859 arg1Of dilution,200
R16284 T21860 T21861 arg1Of dilution,of
R16285 T21860 T21864 arg1Of dilution,in
R16286 T21860 T21876 arg1Of dilution,were
R16287 T21860 T21877 arg2Of dilution,developed
R16288 T21860 T21888 arg2Of dilution,visualized
R16289 T21863 T21861 arg2Of anti-FOXP3,of
R16290 T21863 T21862 arg1Of anti-FOXP3,goat
R16291 T21865 T21864 arg2Of blocking,in
R16292 T21865 T21870 arg1Of blocking,overnight
R16293 T21865 T21871 arg1Of blocking,at
R16294 T21866 T21865 arg2Of buffer,blocking
R16295 T21866 T21867 arg1Of buffer,(
R16296 T21868 T21867 arg2Of Abcam,(
R16297 T21869 T21867 arg3Of ),(
R16298 T21873 T21872 arg1Of °C.,4
R16299 T21875 T21871 arg2Of blots,at
R16300 T21875 T21873 arg1Of blots,°C.
R16301 T21875 T21874 arg1Of blots,The
R16302 T21877 T21887 arg1Of developed,and
R16303 T21878 T21877 arg3Of using,developed
R16304 T21882 T21878 arg2Of mAb,using
R16305 T21882 T21879 arg1Of mAb,an
R16306 T21882 T21880 arg1Of mAb,anti-goat
R16307 T21882 T21881 arg1Of mAb,HRP-labeled
R16308 T21882 T21883 arg1Of mAb,(
R16309 T21885 T21883 arg2Of Biosciences,(
R16310 T21885 T21884 arg1Of Biosciences,Amersham
R16311 T21886 T21883 arg3Of ),(
R16312 T21887 T21858 arg2Of and,:
R16313 T21887 T21876 arg2Of and,were
R16314 T21888 T21887 arg2Of visualized,and
R16315 T21888 T21889 arg1Of visualized,with
R16316 T21893 T21889 arg2Of camera,with
R16317 T21893 T21890 arg1Of camera,a
R16318 T21893 T21891 arg1Of camera,LAS
R16319 T21893 T21892 arg1Of camera,1000
R16320 T21893 T21894 arg1Of camera,(
R16321 T21895 T21894 arg2Of Fuji,(
R16322 T21896 T21894 arg3Of ),(
R16323 T21897 T21898 arg1Of Membranes,were
R16324 T21897 T21899 arg2Of Membranes,incubated
R16325 T21897 T21904 arg1Of Membranes,re-blocked
R16326 T21899 T21900 arg1Of incubated,in
R16327 T21899 T21903 arg1Of incubated,and
R16328 T21901 T21900 arg2Of stripping,in
R16329 T21902 T21901 arg2Of buffer,stripping
R16330 T21903 T21898 arg2Of and,were
R16331 T21904 T21903 arg2Of re-blocked,and
R16332 T21904 T21905 arg1Of re-blocked,for
R16333 T21907 T21905 arg2Of h,for
R16334 T21907 T21906 arg1Of h,1
R16335 T21909 T21908 arg1Of membranes,The
R16336 T21909 T21910 arg1Of membranes,were
R16337 T21909 T21911 arg2Of membranes,re-probed
R16338 T21911 T21910 arg2Of re-probed,were
R16339 T21911 T21955 arg1Of re-probed,","
R16340 T21912 T21911 arg3Of using,re-probed
R16341 T21913 T21914 arg1Of anti-GATA3,(
R16342 T21913 T21921 arg1Of anti-GATA3,","
R16343 T21915 T21914 arg2Of HG3–31,(
R16344 T21915 T21916 arg1Of HG3–31,;
R16345 T21919 T21916 arg2Of Biotechnology,;
R16346 T21919 T21917 arg1Of Biotechnology,Santa
R16347 T21919 T21918 arg1Of Biotechnology,Cruz
R16348 T21920 T21914 arg3Of ),(
R16349 T21921 T21948 arg1Of ",",and
R16350 T21922 T21923 arg1Of anti-T-bet,(
R16351 T21922 T21930 arg1Of anti-T-bet,","
R16352 T21924 T21923 arg2Of 4B10,(
R16353 T21924 T21925 arg1Of 4B10,","
R16354 T21928 T21925 arg2Of Biotechnology,","
R16355 T21928 T21926 arg1Of Biotechnology,Santa
R16356 T21928 T21927 arg1Of Biotechnology,Cruz
R16357 T21929 T21923 arg3Of ),(
R16358 T21930 T21921 arg2Of ",",","
R16359 T21931 T21932 arg1Of anti-GAPDH,(
R16360 T21931 T21937 arg1Of anti-GAPDH,","
R16361 T21933 T21932 arg2Of 6C5,(
R16362 T21933 T21934 arg1Of 6C5,","
R16363 T21933 T21935 arg1Of 6C5,Ambion
R16364 T21936 T21932 arg3Of ),(
R16365 T21937 T21942 arg1Of ",",","
R16366 T21938 T21937 arg2Of anti-phospho-SMAD2,","
R16367 T21938 T21939 arg1Of anti-phospho-SMAD2,(
R16368 T21940 T21939 arg2Of 138D4,(
R16369 T21941 T21939 arg3Of ),(
R16370 T21942 T21930 arg2Of ",",","
R16371 T21943 T21942 arg2Of anti-phospho-STAT6,","
R16372 T21943 T21944 arg1Of anti-phospho-STAT6,(
R16373 T21945 T21944 arg2Of 5A4,(
R16374 T21946 T21944 arg3Of ),(
R16375 T21948 T21912 arg2Of and,using
R16376 T21948 T21947 arg1Of and,","
R16377 T21949 T21948 arg2Of anti-STAT6,and
R16378 T21949 T21950 arg1Of anti-STAT6,(
R16379 T21953 T21950 arg2Of Technology,(
R16380 T21953 T21951 arg1Of Technology,Cell
R16381 T21953 T21952 arg1Of Technology,Signaling
R16382 T21954 T21950 arg3Of ),(
R16570 T22260 T22261 arg1Of Administration,of
R16571 T22262 T22263 arg1Of cytokines,and
R16572 T22263 T22261 arg2Of and,of
R16573 T22264 T22263 arg2Of antibodies,and
R16574 T22264 T22266 arg1Of antibodies,vivo
R16575 T22266 T22265 arg1Of vivo,in
R16576 T22272 T22267 arg1Of mice,Age-
R16577 T22272 T22268 arg1Of mice,and
R16578 T22272 T22269 arg1Of mice,gender-matched
R16579 T22272 T22270 arg1Of mice,normal
R16580 T22272 T22271 arg1Of mice,B6
R16581 T22272 T22273 arg1Of mice,received
R16582 T22273 T22276 arg1Of received,day
R16583 T22273 T22316 arg1Of received,for
R16584 T22276 T22274 arg1Of day,every
R16585 T22276 T22275 arg1Of day,other
R16586 T22277 T22278 arg1Of intraperitoneal,(
R16587 T22279 T22278 arg2Of ip,(
R16588 T22280 T22278 arg3Of ),(
R16589 T22281 T22277 arg1Of injections,intraperitoneal
R16590 T22281 T22282 arg1Of injections,of
R16591 T22281 T22299 arg1Of injections,or
R16592 T22283 T22282 arg2Of PBS,of
R16593 T22283 T22284 arg1Of PBS,","
R16594 T22287 T22284 arg2Of rmIL-4,","
R16595 T22287 T22285 arg1Of rmIL-4,1.5
R16596 T22287 T22286 arg1Of rmIL-4,μg
R16597 T22287 T22288 arg1Of rmIL-4,","
R16598 T22289 T22290 arg1Of 50,μg
R16599 T22290 T22288 arg2Of μg,","
R16600 T22290 T22293 arg1Of μg,(
R16601 T22292 T22290 arg2Of mAb,μg
R16602 T22292 T22291 arg1Of mAb,anti-IL-4
R16603 T22294 T22295 arg1Of 11B11,or
R16604 T22295 T22293 arg2Of or,(
R16605 T22296 T22295 arg2Of MAB404,or
R16606 T22297 T22293 arg3Of ),(
R16607 T22299 T22298 arg1Of or,","
R16608 T22299 T22306 arg1Of or,plus
R16609 T22301 T22299 arg2Of mixture,or
R16610 T22301 T22300 arg1Of mixture,a
R16611 T22301 T22302 arg1Of mixture,of
R16612 T22303 T22304 arg1Of 1.5,μg
R16613 T22305 T22302 arg2Of rmIL-4,of
R16614 T22305 T22303 arg1Of rmIL-4,1.5
R16615 T22306 T22273 arg2Of plus,received
R16616 T22307 T22308 arg1Of 50,μg
R16617 T22309 T22308 arg2Of anti-IL-4,μg
R16618 T22310 T22306 arg2Of mAb,plus
R16619 T22310 T22307 arg1Of mAb,50
R16620 T22310 T22309 arg1Of mAb,anti-IL-4
R16621 T22310 T22311 arg1Of mAb,(
R16622 T22312 T22313 arg1Of 11B11,or
R16623 T22313 T22311 arg2Of or,(
R16624 T22314 T22313 arg2Of MAB404,or
R16625 T22315 T22311 arg3Of ),(
R16626 T22318 T22316 arg2Of d,for
R16627 T22318 T22317 arg1Of d,7
R16628 T22321 T22322 arg1Of spleen,and
R16629 T22323 T22322 arg2Of lymph,and
R16630 T22325 T22321 arg1Of cells,spleen
R16631 T22325 T22323 arg1Of cells,lymph
R16632 T22325 T22324 arg1Of cells,node
R16633 T22325 T22326 arg1Of cells,were
R16634 T22325 T22327 arg2Of cells,analyzed
R16635 T22327 T22319 arg1Of analyzed,Thereafter
R16636 T22327 T22320 arg1Of analyzed,","
R16637 T22327 T22326 arg2Of analyzed,were
R16638 T22327 T22328 arg1Of analyzed,by
R16639 T22330 T22328 arg2Of cytometry,by
R16640 T22330 T22329 arg1Of cytometry,flow
R16641 T22330 T22331 arg1Of cytometry,for
R16642 T22332 T22333 arg1Of CD3,","
R16643 T22333 T22336 arg1Of ",",and
R16644 T22334 T22333 arg2Of CD4,","
R16645 T22336 T22331 arg2Of and,for
R16646 T22336 T22335 arg1Of and,","
R16647 T22338 T22336 arg2Of expression,and
R16648 T22338 T22337 arg1Of expression,CD25
R16649 T22343 T22339 arg1Of MAB404,The
R16650 T22343 T22340 arg1Of MAB404,anti-mouse
R16651 T22343 T22341 arg1Of MAB404,IL-4
R16652 T22343 T22342 arg1Of MAB404,mAb
R16653 T22343 T22344 arg1Of MAB404,was
R16654 T22343 T22345 arg2Of MAB404,obtained
R16655 T22345 T22344 arg2Of obtained,was
R16656 T22345 T22346 arg1Of obtained,from
R16657 T22350 T22346 arg2Of Systems,from
R16658 T22350 T22347 arg1Of Systems,R
R16659 T22350 T22348 arg1Of Systems,&
R16660 T22350 T22349 arg1Of Systems,D
R16661 T22357 T22352 arg1Of 11B11,the
R16662 T22357 T22353 arg1Of 11B11,second
R16663 T22357 T22354 arg1Of 11B11,anti-mouse
R16664 T22357 T22355 arg1Of 11B11,IL-4
R16665 T22357 T22356 arg1Of 11B11,mAb
R16666 T22357 T22358 arg1Of 11B11,was
R16667 T22357 T22359 arg2Of 11B11,purchased
R16668 T22359 T22344 modOf purchased,was
R16669 T22359 T22351 arg1Of purchased,","
R16670 T22359 T22358 arg2Of purchased,was
R16671 T22359 T22360 arg1Of purchased,from
R16672 T22361 T22360 arg2Of eBioscience,from
R16797 T22576 T22575 arg1Of analysis,ChIP
R16798 T22576 T22577 arg1Of analysis,was
R16799 T22576 T22578 arg2Of analysis,performed
R16800 T22578 T22577 arg2Of performed,was
R16801 T22578 T22579 arg1Of performed,according
R16802 T22578 T22594 arg1Of performed,and
R16803 T22580 T22579 arg2Of to,according
R16804 T22582 T22581 arg1Of manufacturer,the
R16805 T22582 T22583 arg2Of manufacturer,'s
R16806 T22584 T22580 arg2Of protocol,to
R16807 T22584 T22583 arg1Of protocol,'s
R16808 T22584 T22585 arg1Of protocol,(
R16809 T22584 T22589 arg1Of protocol,with
R16810 T22587 T22585 arg2Of Biotechnology,(
R16811 T22587 T22586 arg1Of Biotechnology,Upstate
R16812 T22588 T22585 arg3Of ),(
R16813 T22593 T22589 arg2Of iTreg,with
R16814 T22593 T22590 arg1Of iTreg,the
R16815 T22593 T22591 arg1Of iTreg,following
R16816 T22593 T22592 arg1Of iTreg,modifications.
R16817 T22596 T22595 arg1Of cells,Th2
R16818 T22596 T22597 arg1Of cells,were
R16819 T22596 T22598 arg2Of cells,fixed
R16820 T22598 T22594 arg2Of fixed,and
R16821 T22598 T22597 arg2Of fixed,were
R16822 T22598 T22599 arg1Of fixed,with
R16823 T22598 T22603 arg1Of fixed,for
R16824 T22600 T22601 arg1Of 1,%
R16825 T22602 T22599 arg2Of formaldehyde,with
R16826 T22602 T22600 arg1Of formaldehyde,1
R16827 T22605 T22603 arg2Of min,for
R16828 T22605 T22604 arg1Of min,10
R16829 T22605 T22606 arg1Of min,at
R16830 T22608 T22606 arg2Of temperature,at
R16831 T22608 T22607 arg1Of temperature,room
R16832 T22610 T22609 arg1Of chromatin,The
R16833 T22610 T22611 arg1Of chromatin,was
R16834 T22610 T22612 arg2Of chromatin,sheared
R16835 T22612 T22611 arg2Of sheared,was
R16836 T22612 T22613 arg1Of sheared,to
R16837 T22612 T22620 arg1Of sheared,with
R16838 T22615 T22613 arg2Of bp,to
R16839 T22615 T22614 arg1Of bp,200-1000
R16840 T22615 T22616 arg1Of bp,of
R16841 T22617 T22616 arg2Of length,of
R16842 T22617 T22618 arg1Of length,by
R16843 T22619 T22618 arg2Of sonication,by
R16844 T22622 T22620 arg2Of pulses,with
R16845 T22622 T22621 arg1Of pulses,five
R16846 T22622 T22623 arg1Of pulses,of
R16847 T22625 T22623 arg2Of s,of
R16848 T22625 T22624 arg1Of s,10
R16849 T22625 T22626 arg1Of s,at
R16850 T22627 T22628 arg1Of 30,%
R16851 T22629 T22626 arg2Of power,at
R16852 T22629 T22627 arg1Of power,30
R16853 T22629 T22630 arg1Of power,(
R16854 T22631 T22630 arg2Of Bandelin,(
R16855 T22632 T22630 arg3Of ),(
R16856 T22634 T22633 arg1Of chromatin,The
R16857 T22634 T22635 arg1Of chromatin,was
R16858 T22634 T22636 arg1Of chromatin,pre-cleared
R16859 T22634 T22647 arg1Of chromatin,incubated
R16860 T22635 T22645 arg1Of was,and
R16861 T22636 T22635 arg2Of pre-cleared,was
R16862 T22636 T22637 arg1Of pre-cleared,for
R16863 T22639 T22637 arg2Of h,for
R16864 T22639 T22638 arg1Of h,2
R16865 T22639 T22640 arg1Of h,with
R16866 T22644 T22640 arg2Of beads,with
R16867 T22644 T22641 arg1Of beads,normal
R16868 T22644 T22642 arg1Of beads,mouse
R16869 T22644 T22643 arg1Of beads,IgG
R16870 T22647 T22645 arg2Of incubated,and
R16871 T22647 T22646 arg1Of incubated,then
R16872 T22647 T22648 arg1Of incubated,with
R16873 T22647 T22658 arg1Of incubated,for
R16874 T22650 T22648 arg2Of beads,with
R16875 T22650 T22649 arg1Of beads,anti-GATA3-agarose
R16876 T22650 T22651 arg1Of beads,(
R16877 T22652 T22651 arg2Of HG3–31,(
R16878 T22652 T22653 arg1Of HG3–31,;
R16879 T22656 T22653 arg2Of Biotechnology,;
R16880 T22656 T22654 arg1Of Biotechnology,Santa
R16881 T22656 T22655 arg1Of Biotechnology,Cruz
R16882 T22657 T22651 arg3Of ),(
R16883 T22660 T22658 arg2Of h,for
R16884 T22660 T22659 arg1Of h,2
R16885 T22661 T22675 arg1Of Washing,were
R16886 T22661 T22676 arg2Of Washing,reversed
R16887 T22664 T22662 arg1Of buffers,and
R16888 T22664 T22663 arg1Of buffers,elution
R16889 T22664 T22665 arg1Of buffers,were
R16890 T22664 T22666 arg2Of buffers,used
R16891 T22666 T22665 arg2Of used,were
R16892 T22666 T22667 arg1Of used,according
R16893 T22668 T22667 arg2Of to,according
R16894 T22670 T22668 arg2Of protocol,to
R16895 T22670 T22669 arg1Of protocol,the
R16896 T22670 T22671 arg1Of protocol,of
R16897 T22674 T22671 arg2Of Crosslinks,of
R16898 T22674 T22672 arg1Of Crosslinks,Upstate
R16899 T22674 T22673 arg1Of Crosslinks,Biotechnology.
R16900 T22676 T22665 modOf reversed,were
R16901 T22676 T22675 arg2Of reversed,were
R16902 T22676 T22679 arg1Of reversed,at
R16903 T22676 T22682 arg1Of reversed,for
R16904 T22676 T22685 arg1Of reversed,in
R16905 T22676 T22693 arg1Of reversed,and
R16906 T22678 T22676 arg1Of incubation,reversed
R16907 T22678 T22677 arg2Of incubation,by
R16908 T22681 T22679 arg2Of °C,at
R16909 T22681 T22680 arg1Of °C,65
R16910 T22684 T22682 arg2Of h,for
R16911 T22684 T22683 arg1Of h,4
R16912 T22687 T22685 arg2Of presence,in
R16913 T22687 T22686 arg1Of presence,the
R16914 T22687 T22688 arg1Of presence,of
R16915 T22689 T22690 arg1Of 0.2,M
R16916 T22691 T22688 arg2Of NaCl,of
R16917 T22691 T22689 arg1Of NaCl,0.2
R16918 T22693 T22692 arg1Of and,","
R16919 T22695 T22694 arg1Of DNA,the
R16920 T22695 T22696 arg1Of DNA,was
R16921 T22695 T22697 arg2Of DNA,purified
R16922 T22697 T22693 arg2Of purified,and
R16923 T22697 T22696 arg2Of purified,was
R16924 T22700 T22697 arg1Of extraction,purified
R16925 T22700 T22698 arg2Of extraction,by
R16926 T22700 T22699 arg1Of extraction,phenol/chloroform
R16927 T22702 T22701 arg1Of amount,The
R16928 T22702 T22703 arg1Of amount,of
R16929 T22702 T22705 arg1Of amount,was
R16930 T22702 T22706 arg2Of amount,determined
R16931 T22704 T22703 arg2Of DNA,of
R16932 T22706 T22705 arg2Of determined,was
R16933 T22709 T22706 arg1Of PCR,determined
R16934 T22709 T22707 arg2Of PCR,by
R16935 T22709 T22708 arg1Of PCR,conventional
R16936 T22711 T22710 arg1Of PCR,The
R16937 T22711 T22712 arg2Of PCR,addressed
R16938 T22711 T22722 arg1Of PCR,was
R16939 T22711 T22723 arg2Of PCR,performed
R16940 T22712 T22713 arg1Of addressed,for
R16941 T22717 T22713 arg2Of region,for
R16942 T22717 T22714 arg1Of region,the
R16943 T22717 T22715 arg1Of region,FOXP3
R16944 T22717 T22716 arg1Of region,promoter
R16945 T22717 T22718 arg1Of region,−246
R16946 T22717 T22719 arg1Of region,to
R16947 T22720 T22719 arg2Of −511,to
R16948 T22720 T22721 arg1Of −511,and
R16949 T22723 T22722 arg2Of performed,was
R16950 T22724 T22723 arg3Of using,performed
R16951 T22727 T22724 arg2Of primers,using
R16952 T22727 T22725 arg1Of primers,the
R16953 T22727 T22726 arg1Of primers,following
R16954 T22727 T22728 arg1Of primers,:
R16955 T22730 T22731 arg1Of catctg-3′,and
R16956 T22731 T22729 arg1Of and,5′-gtgccctttacgagt
R16957 T22732 T22731 arg2Of 5′-gtgccctttacgagtcatctg-3′,and
R16958 T22735 T22733 arg1Of products,The
R16959 T22735 T22734 arg1Of products,PCR
R16960 T22735 T22736 arg1Of products,were
R16961 T22735 T22737 arg2Of products,visualized
R16962 T22737 T22736 arg2Of visualized,were
R16963 T22738 T22737 arg3Of using,visualized
R16964 T22742 T22738 arg2Of gel,using
R16965 T22742 T22739 arg1Of gel,an
R16966 T22742 T22740 arg1Of gel,ethidium
R16967 T22742 T22741 arg1Of gel,bromide
R17158 T22976 T22975 arg1Of assay,Pull-down
R17159 T22979 T22977 arg1Of cells,CD4+
R17160 T22979 T22978 arg1Of cells,T
R17161 T22979 T22980 arg1Of cells,were
R17162 T22979 T22981 arg2Of cells,stimulated
R17163 T22981 T22980 arg2Of stimulated,were
R17164 T22981 T22982 arg1Of stimulated,with
R17165 T22983 T22984 arg1Of PMA,and
R17166 T22984 T22982 arg2Of and,with
R17167 T22984 T22986 arg1Of and,for
R17168 T22984 T22989 arg1Of and,at
R17169 T22985 T22984 arg2Of ionomycin,and
R17170 T22988 T22986 arg2Of h,for
R17171 T22988 T22987 arg1Of h,2
R17172 T22990 T22989 arg2Of 37°C.,at
R17173 T22992 T22991 arg1Of cells,The
R17174 T22992 T22993 arg1Of cells,were
R17175 T22992 T22994 arg2Of cells,pelleted
R17176 T22992 T22996 arg2Of cells,resuspended
R17177 T22992 T23036 arg2Of cells,) and
R17178 T22994 T22995 arg1Of pelleted,","
R17179 T22995 T23000 arg1Of ",",(
R17180 T22995 T23035 arg1Of ",",-40
R17181 T22996 T22995 arg2Of resuspended,","
R17182 T22996 T22997 arg1Of resuspended,in
R17183 T22999 T22997 arg2Of C,in
R17184 T22999 T22998 arg1Of C,buffer
R17185 T23001 T23002 arg1Of 20,mM
R17186 T23003 T23001 arg1Of HEPES,20
R17187 T23003 T23004 arg1Of HEPES,[
R17188 T23003 T23008 arg1Of HEPES,","
R17189 T23005 T23004 arg2Of pH,[
R17190 T23005 T23006 arg1Of pH,7.9
R17191 T23007 T23004 arg3Of ],[
R17192 T23008 T23024 arg1Of ",",","
R17193 T23009 T23010 arg1Of 420,mM
R17194 T23011 T23009 arg1Of NaCl,420
R17195 T23011 T23012 arg1Of NaCl,","
R17196 T23012 T23016 arg1Of ",",","
R17197 T23013 T23014 arg1Of 1.5,mM
R17198 T23015 T23012 arg2Of MgCl2,","
R17199 T23015 T23013 arg1Of MgCl2,1.5
R17200 T23016 T23020 arg1Of ",",","
R17201 T23017 T23018 arg1Of 0.2,mM
R17202 T23019 T23016 arg2Of EDTA,","
R17203 T23019 T23017 arg1Of EDTA,0.2
R17204 T23020 T23008 arg2Of ",",","
R17205 T23021 T23022 arg1Of 1,mM
R17206 T23023 T23020 arg2Of DTT,","
R17207 T23023 T23021 arg1Of DTT,1
R17208 T23024 T23030 arg1Of ",",. a
R17209 T23026 T23024 arg2Of inhibitors,","
R17210 T23026 T23025 arg1Of inhibitors,protease
R17211 T23026 T23027 arg1Of inhibitors,[
R17212 T23028 T23027 arg2Of Sigma,[
R17213 T23029 T23027 arg3Of ],[
R17214 T23030 T23000 arg2Of . a,(
R17215 T23031 T23032 arg1Of nd ,0
R17216 T23033 T23030 arg2Of .1% N,. a
R17217 T23033 T23031 arg1Of .1% N,nd
R17218 T23034 T23000 arg3Of P,(
R17219 T23035 T22980 modOf -40,were
R17220 T23035 T22993 arg2Of -40,were
R17221 T23036 T23035 arg2Of ) and,-40
R17222 T23036 T23037 arg1Of ) and,ly
R17223 T23036 T23039 arg1Of ) and,on
R17224 T23038 T23037 arg2Of sed,ly
R17225 T23041 T23039 arg2Of e f,on
R17226 T23041 T23040 arg1Of e f,ic
R17227 T23043 T23042 arg1Of material,Insoluble
R17228 T23043 T23044 arg1Of material,was
R17229 T23043 T23045 arg2Of material,removed
R17230 T23045 T23044 arg2Of removed,was
R17231 T23047 T23045 arg1Of centrifugation,removed
R17232 T23047 T23046 arg2Of centrifugation,by
R17233 T23049 T23048 arg1Of supernatant,The
R17234 T23049 T23050 arg1Of supernatant,was
R17235 T23049 T23051 arg2Of supernatant,diluted
R17236 T23051 T23050 arg2Of diluted,was
R17237 T23051 T23052 arg1Of diluted,1
R17238 T23052 T23053 arg1Of 1,:
R17239 T23054 T23053 arg2Of 3,:
R17240 T23054 T23055 arg1Of 3,with
R17241 T23054 T23058 arg1Of 3,(
R17242 T23057 T23055 arg2Of D,with
R17243 T23057 T23056 arg1Of D,buffer
R17244 T23059 T23063 arg1Of as,but
R17245 T23061 T23059 arg2Of C,as
R17246 T23061 T23060 arg1Of C,buffer
R17247 T23063 T23058 arg2Of but,(
R17248 T23063 T23062 arg1Of but,","
R17249 T23064 T23063 arg2Of without,but
R17250 T23065 T23064 arg2Of NaCl,without
R17251 T23066 T23058 arg3Of ),(
R17252 T23068 T23067 arg1Of lysates,The
R17253 T23068 T23069 arg1Of lysates,were
R17254 T23068 T23070 arg2Of lysates,incubated
R17255 T23068 T23117 arg1Of lysates,resuspended
R17256 T23070 T23071 arg1Of incubated,with
R17257 T23070 T23098 arg1Of incubated,at
R17258 T23070 T23116 arg1Of incubated,and
R17259 T23073 T23071 arg2Of μg,with
R17260 T23073 T23072 arg1Of μg,10
R17261 T23073 T23074 arg1Of μg,of
R17262 T23075 T23076 arg1Of poly,(
R17263 T23075 T23079 arg1Of poly,(
R17264 T23075 T23082 arg1Of poly,and
R17265 T23077 T23076 arg2Of dI-dC,(
R17266 T23078 T23076 arg3Of ),(
R17267 T23080 T23079 arg2Of Sigma,(
R17268 T23081 T23079 arg3Of ),(
R17269 T23082 T23074 arg2Of and,of
R17270 T23084 T23082 arg2Of μl,and
R17271 T23084 T23083 arg1Of μl,70
R17272 T23084 T23085 arg1Of μl,of
R17273 T23089 T23087 arg2Of Biosciences,(
R17274 T23089 T23088 arg1Of Biosciences,Amersham
R17275 T23090 T23087 arg3Of ),(
R17276 T23093 T23085 arg2Of oligonucleotides,of
R17277 T23093 T23086 arg1Of oligonucleotides,streptavidin-agarose
R17278 T23093 T23087 arg1Of oligonucleotides,(
R17279 T23093 T23091 arg1Of oligonucleotides,carrying
R17280 T23093 T23092 arg2Of oligonucleotides,biotinylated
R17281 T23093 T23094 arg1Of oligonucleotides,","
R17282 T23093 T23095 arg1Of oligonucleotides,for
R17283 T23097 T23095 arg2Of h,for
R17284 T23097 T23096 arg1Of h,3
R17285 T23100 T23099 arg1Of °C.,4
R17286 T23100 T23103 modOf °C.,were
R17287 T23102 T23101 arg1Of beads,The
R17288 T23102 T23103 arg1Of beads,were
R17289 T23102 T23104 arg2Of beads,washed
R17290 T23104 T23103 arg2Of washed,were
R17291 T23104 T23105 arg1Of washed,twice
R17292 T23104 T23106 arg1Of washed,with
R17293 T23104 T23109 arg1Of washed,:
R17294 T23108 T23106 arg2Of C,with
R17295 T23108 T23107 arg1Of C,buffer
R17296 T23110 T23098 arg2Of D,at
R17297 T23110 T23100 arg1Of D,°C.
R17298 T23110 T23111 arg1Of D,(
R17299 T23112 T23113 arg1Of 1,:
R17300 T23113 T23111 arg2Of :,(
R17301 T23114 T23113 arg2Of 3,:
R17302 T23115 T23111 arg3Of ),(
R17303 T23116 T23069 arg2Of and,were
R17304 T23117 T23116 arg2Of resuspended,and
R17305 T23117 T23118 arg1Of resuspended,in
R17306 T23121 T23119 arg1Of buffer,DTT-containing
R17307 T23121 T23120 arg1Of buffer,loading
R17308 T23121 T23122 arg1Of buffer,(
R17309 T23121 T23127 arg1Of buffer,","
R17310 T23121 T23128 arg2Of buffer,heated
R17311 T23121 T23136 arg1Of buffer,and
R17312 T23123 T23122 arg2Of NuPAGE,(
R17313 T23123 T23124 arg1Of NuPAGE,;
R17314 T23125 T23124 arg2Of Invitrogen,;
R17315 T23126 T23122 arg3Of ),(
R17316 T23128 T23129 arg1Of heated,to
R17317 T23128 T23132 arg1Of heated,for
R17318 T23131 T23129 arg2Of °C,to
R17319 T23131 T23130 arg1Of °C,70
R17320 T23134 T23132 arg2Of min,for
R17321 T23134 T23133 arg1Of min,10
R17322 T23136 T23118 arg2Of and,in
R17323 T23136 T23135 arg1Of and,","
R17324 T23138 T23136 arg2Of eluants,and
R17325 T23138 T23137 arg1Of eluants,the
R17326 T23138 T23139 arg1Of eluants,on
R17327 T23142 T23143 arg1Of 4–12,%
R17328 T23145 T23139 arg2Of gel,on
R17329 T23145 T23140 arg1Of gel,a
R17330 T23145 T23141 arg1Of gel,NuPAGE
R17331 T23145 T23142 arg1Of gel,4–12
R17332 T23145 T23144 arg1Of gel,bis-tris
R17333 T23145 T23146 arg1Of gel,(
R17334 T23147 T23146 arg2Of Invitrogen,(
R17335 T23148 T23146 arg3Of ),(
R17336 T23150 T23149 arg1Of proteins,The
R17337 T23150 T23151 arg1Of proteins,were
R17338 T23150 T23152 arg2Of proteins,electroblotted
R17339 T23150 T23162 arg2Of proteins,detected
R17340 T23150 T23163 arg1Of proteins,using
R17341 T23152 T23153 arg1Of electroblotted,onto
R17342 T23152 T23161 arg1Of electroblotted,and
R17343 T23156 T23153 arg2Of membrane,onto
R17344 T23156 T23154 arg1Of membrane,a
R17345 T23156 T23155 arg1Of membrane,PVDF
R17346 T23156 T23157 arg1Of membrane,(
R17347 T23159 T23157 arg2Of Biosciences,(
R17348 T23159 T23158 arg1Of Biosciences,Amersham
R17349 T23160 T23157 arg3Of ),(
R17350 T23161 T23151 arg2Of and,were
R17351 T23162 T23161 arg2Of detected,and
R17352 T23163 T23162 arg3Of using,detected
R17353 T23166 T23163 arg2Of mAb,using
R17354 T23166 T23164 arg1Of mAb,an
R17355 T23166 T23165 arg1Of mAb,anti-GATA3
R17356 T23166 T23167 arg1Of mAb,(
R17357 T23170 T23167 arg2Of Biotechnology,(
R17358 T23170 T23168 arg1Of Biotechnology,Santa
R17359 T23170 T23169 arg1Of Biotechnology,Cruz
R17360 T23171 T23167 arg3Of ),(
R17361 T23173 T23172 arg2Of signals,Accumulated
R17362 T23173 T23174 arg1Of signals,were
R17363 T23173 T23175 arg2Of signals,analyzed
R17364 T23175 T23174 arg2Of analyzed,were
R17365 T23176 T23175 arg3Of using,analyzed
R17366 T23178 T23176 arg2Of software,using
R17367 T23178 T23177 arg1Of software,AIDA
R17368 T23178 T23179 arg1Of software,(
R17369 T23180 T23179 arg2Of Raytest,(
R17370 T23181 T23179 arg3Of ),(
R13422 T17783 T17784 arg1Of purchased,from
R13424 T17787 T17784 arg2Of Laboratories,from
R13425 T17787 T17785 arg1Of Laboratories,the
R13426 T17787 T17786 arg1Of Laboratories,Jackson
R13427 T17787 T17788 arg1Of Laboratories,(
R14624 T19522 T19523 arg1Of Cloning,of
R14625 T19522 T19527 arg1Of Cloning,and
R14626 T19526 T19523 arg2Of promoter,of
R14627 T19526 T19524 arg1Of promoter,the
R14628 T19526 T19525 arg1Of promoter,FOXP3
R14629 T19528 T19527 arg2Of construction,and
R14630 T19528 T19529 arg1Of construction,of
R14631 T19531 T19529 arg2Of constructs,of
R14632 T19531 T19530 arg1Of constructs,mutant
R14633 T19534 T19532 arg1Of promoter,The
R14634 T19534 T19533 arg1Of promoter,FOXP3
R14635 T19534 T19535 arg1Of promoter,was
R15011 T20052 T20051 arg1Of isolation,RNA
R15012 T20052 T20053 arg1Of isolation,and
R15013 T20055 T20053 arg2Of synthesis,and
R15014 T20055 T20054 arg1Of synthesis,cDNA
R15015 T20056 T20057 arg1Of RNA,was
R15016 T20056 T20058 arg2Of RNA,isolated

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T17891 23-27 NNP denotes Mice
T17892 27-28 . denotes .
T17893 29-35 JJ denotes Normal
T17894 36-38 NN denotes B6
T17895 39-43 NNS denotes mice
T17896 44-48 VBD denotes were
T17897 49-58 VBN denotes purchased
T17898 59-63 IN denotes from
T17899 64-67 DT denotes the
T17900 68-75 NNP denotes Jackson
T17901 76-88 NNP denotes Laboratories
T17902 89-90 -LRB- denotes (
T17903 90-93 NNP denotes Bar
T17904 94-100 NNP denotes Harbor
T17905 100-101 , denotes ,
T17906 102-107 NNP denotes Maine
T17907 107-108 -RRB- denotes )
T17908 108-109 . denotes .
T17909 110-120 NNP denotes Transgenic
T17910 121-125 NNP denotes DO11
T17911 125-128 CD denotes .10
T17912 129-133 NNS denotes mice
T17913 133-134 , denotes ,
T17914 135-145 VBG denotes expressing
T17915 146-147 DT denotes a
T17916 148-149 NNP denotes T
T17917 150-154 NN denotes cell
T17918 155-163 NN denotes receptor
T17919 164-167 IN denotes for
T17920 168-174 NNP denotes OVA323
T17921 175-178 CD denotes 339
T17922 179-186 NN denotes peptide
T17923 187-189 IN denotes in
T17924 190-193 DT denotes the
T17925 194-201 NN denotes context
T17926 202-204 IN denotes of
T17927 205-209 JJ denotes H-2d
T17928 209-210 , denotes ,
T17929 211-215 VBD denotes were
T17930 216-227 VBN denotes backcrossed
T17931 228-232 IN denotes with
T17932 233-237 NNS denotes mice
T17933 238-248 VBG denotes expressing
T17934 249-255 NNP denotes GATA-3
T17935 255-256 , denotes ,
T17936 257-263 VBN denotes driven
T17937 264-266 IN denotes by
T17938 267-270 DT denotes the
T17939 271-276 JJ denotes human
T17940 277-280 NNP denotes CD2
T17941 281-286 NN denotes locus
T17942 287-294 NN denotes control
T17943 295-301 NN denotes region
T17944 302-303 -LRB- denotes (
T17945 303-312 NN denotes CD2-GATA3
T17946 312-313 -RRB- denotes )
T17947 314-315 NNP denotes [
T17948 315-317 CD denotes 22
T17949 317-318 NNP denotes ]
T17950 318-319 , denotes ,
T17951 320-329 VBG denotes resulting
T17952 330-332 IN denotes in
T17953 333-350 JJ denotes DO11.10xCD2-GATA3
T17954 351-355 NNS denotes mice
T17955 355-356 . denotes .
T17956 357-361 NN denotes Mice
T17957 362-366 VBN denotes used
T17958 367-370 IN denotes for
T17959 371-382 NNS denotes experiments
T17960 383-387 VBD denotes were
T17961 388-399 VBN denotes backcrossed
T17962 400-402 IN denotes on
T17963 403-404 DT denotes a
T17964 405-411 NNP denotes BALB/c
T17965 412-422 NN denotes background
T17966 423-426 IN denotes for
T17967 427-428 DT denotes a
T17968 429-436 NN denotes minimum
T17969 437-439 IN denotes of
T17970 440-445 CD denotes eight
T17971 446-457 NNS denotes generations
T17972 458-461 CC denotes and
T17973 462-466 VBN denotes used
T17974 467-469 IN denotes at
T17975 470-472 DT denotes an
T17976 473-476 NN denotes age
T17977 477-479 IN denotes of
T17978 480-481 CD denotes 8
T17979 482-484 CD denotes 12
T17980 485-487 NN denotes wk
T17981 487-488 . denotes .
T17982 489-493 NNS denotes Mice
T17983 494-498 VBD denotes were
T17984 499-505 VBN denotes housed
T17985 506-511 IN denotes under
T17986 512-520 JJ denotes specific
T17987 521-534 JJ denotes pathogen-free
T17988 535-545 NNS denotes conditions
T17989 546-549 CC denotes and
T17990 550-553 DT denotes all
T17991 554-560 JJ denotes animal
T17992 561-568 NNS denotes studies
T17993 569-573 VBD denotes were
T17994 574-583 VBN denotes performed
T17995 584-593 VBG denotes according
T17996 594-596 TO denotes to
T17997 597-610 JJ denotes institutional
T17998 611-614 CC denotes and
T17999 615-620 NN denotes state
T18000 621-631 NNS denotes guidelines
T18001 631-632 . denotes .
T18166 634-643 NN denotes Isolation
T18167 644-646 IN denotes of
T18168 647-650 CD denotes CD4
T18169 650-651 CD denotes +
T18170 652-653 NNP denotes T
T18171 654-659 NNS denotes cells
T18172 659-660 . denotes .
T18173 661-664 CD denotes CD4
T18174 664-665 CD denotes +
T18175 666-667 NNP denotes T
T18176 668-673 NNS denotes cells
T18177 674-678 VBD denotes were
T18178 679-687 VBN denotes isolated
T18179 688-692 IN denotes from
T18180 693-698 NN denotes blood
T18181 699-701 IN denotes of
T18182 702-709 JJ denotes healthy
T18183 710-715 JJ denotes human
T18184 716-726 NNS denotes volunteers
T18185 727-732 VBG denotes using
T18186 733-736 DT denotes the
T18187 737-745 JJ denotes anti-CD4
T18188 746-754 JJ denotes magnetic
T18189 755-760 NNS denotes beads
T18190 761-762 -LRB- denotes (
T18191 762-767 NNP denotes Dynal
T18192 767-768 , denotes ,
T18193 769-776 NNP denotes Hamburg
T18194 776-777 , denotes ,
T18195 778-785 NNP denotes Germany
T18196 785-786 -RRB- denotes )
T18197 787-789 IN denotes as
T18198 790-800 RB denotes previously
T18199 801-810 VBN denotes described
T18200 811-812 NNP denotes [
T18201 812-814 CD denotes 60
T18202 814-815 NNP denotes ]
T18203 815-816 . denotes .
T18204 817-820 DT denotes The
T18205 821-827 NN denotes purity
T18206 828-830 IN denotes of
T18207 831-834 CD denotes CD4
T18208 834-835 CD denotes +
T18209 836-837 NNP denotes T
T18210 838-843 NNS denotes cells
T18211 844-847 VBD denotes was
T18212 848-857 RB denotes initially
T18213 858-864 VBN denotes tested
T18214 865-867 IN denotes by
T18215 868-872 NNP denotes FACS
T18216 873-876 CC denotes and
T18217 877-880 VBD denotes was
T18218 881-882 VBN denotes ≥
T18219 883-885 CD denotes 95
T18220 885-886 NN denotes %
T18221 886-887 . denotes .
T18222 888-901 NN denotes Monoclonality
T18223 902-904 IN denotes of
T18224 905-906 NNP denotes T
T18225 907-911 NN denotes cell
T18226 912-918 NNS denotes clones
T18227 919-922 VBD denotes was
T18228 923-932 VBN denotes confirmed
T18229 933-935 IN denotes by
T18230 936-945 JJ denotes TCR-chain
T18231 946-953 NN denotes mapping
T18232 954-957 CC denotes and
T18233 958-961 VBD denotes was
T18234 962-972 VBN denotes identified
T18235 973-975 TO denotes to
T18236 976-978 VB denotes be
T18237 979-985 JJ denotes Vbeta8
T18238 986-994 JJ denotes positive
T18239 994-995 . denotes .
T18240 996-999 DT denotes The
T18241 1000-1006 NNS denotes clones
T18242 1007-1011 VBD denotes were
T18243 1012-1025 VBN denotes characterized
T18244 1026-1028 IN denotes by
T18245 1029-1033 JJ denotes high
T18246 1034-1038 NN denotes IL-4
T18247 1039-1048 NN denotes secretion
T18248 1048-1049 . denotes .
T18362 1051-1063 JJ denotes Quantitative
T18363 1064-1073 JJ denotes real-time
T18364 1074-1077 NNP denotes PCR
T18365 1077-1078 . denotes .
T18366 1079-1082 DT denotes The
T18367 1083-1086 NNP denotes PCR
T18368 1087-1094 NNS denotes primers
T18369 1095-1098 CC denotes and
T18370 1099-1105 NNS denotes probes
T18371 1106-1110 VBD denotes were
T18372 1111-1119 VBN denotes designed
T18373 1120-1125 VBN denotes based
T18374 1126-1128 IN denotes on
T18375 1129-1132 DT denotes the
T18376 1133-1142 NNS denotes sequences
T18377 1143-1151 VBD denotes reported
T18378 1152-1154 IN denotes in
T18379 1155-1162 NNP denotes GenBank
T18380 1163-1167 IN denotes with
T18381 1168-1171 DT denotes the
T18382 1172-1178 NNP denotes Primer
T18383 1179-1186 NNP denotes Express
T18384 1187-1195 NN denotes software
T18385 1196-1203 NN denotes version
T18386 1204-1207 CD denotes 1.2
T18387 1208-1209 -LRB- denotes (
T18388 1209-1216 NNP denotes Applied
T18389 1217-1227 NNPS denotes Biosystems
T18390 1227-1228 -RRB- denotes )
T18391 1229-1231 IN denotes as
T18392 1232-1239 VBZ denotes follows
T18393 1239-1240 : denotes :
T18394 1241-1246 CD denotes FOXP3
T18395 1247-1254 RB denotes forward
T18396 1255-1261 NN denotes primer
T18397 1262-1263 CD denotes 5
T18398 1263-1264 SYM denotes ′
T18399 1264-1265 : denotes -
T18400 1265-1268 NNP denotes GAA
T18401 1269-1272 NNP denotes ACA
T18402 1273-1276 NNP denotes GCA
T18403 1277-1280 NNP denotes CAT
T18404 1281-1284 NNP denotes TCC
T18405 1285-1288 NNP denotes CAG
T18406 1289-1292 NNP denotes AGT
T18407 1293-1297 JJ denotes TC-3
T18408 1297-1298 NN denotes ′
T18409 1298-1299 : denotes ;
T18410 1300-1305 CD denotes FOXP3
T18411 1306-1313 NN denotes reverse
T18412 1314-1320 NN denotes primer
T18413 1321-1322 CD denotes 5
T18414 1322-1323 SYM denotes ′
T18415 1323-1324 : denotes -
T18416 1324-1327 NNP denotes ATG
T18417 1328-1331 NNP denotes GCC
T18418 1332-1335 NNP denotes CAG
T18419 1336-1339 NNP denotes CGG
T18420 1340-1343 NNP denotes ATG
T18421 1344-1348 JJ denotes AG-3
T18422 1348-1349 NN denotes ′
T18423 1349-1350 : denotes ;
T18424 1351-1356 NNP denotes EF-1α
T18425 1357-1364 RB denotes forward
T18426 1365-1371 JJR denotes primer
T18427 1372-1375 CC denotes and
T18428 1376-1383 VB denotes reverse
T18429 1384-1390 NN denotes primer
T18430 1391-1393 IN denotes as
T18431 1394-1403 VBN denotes described
T18432 1404-1405 NNP denotes [
T18433 1405-1407 CD denotes 61
T18434 1407-1408 NNP denotes ]
T18435 1408-1409 : denotes ;
T18436 1410-1415 NNP denotes GATA3
T18437 1416-1423 RB denotes forward
T18438 1424-1430 JJR denotes primer
T18439 1431-1432 CD denotes 5
T18440 1432-1433 SYM denotes ′
T18441 1433-1434 : denotes -
T18442 1434-1437 NNP denotes GCG
T18443 1438-1441 NNP denotes GGC
T18444 1442-1445 NNP denotes TCT
T18445 1446-1449 NNP denotes ATC
T18446 1450-1453 NNP denotes ACA
T18447 1454-1457 NNP denotes AAA
T18448 1458-1463 CD denotes TGA-3
T18449 1463-1464 NN denotes ′
T18450 1465-1468 CC denotes and
T18451 1469-1472 NN denotes rwd
T18452 1473-1474 CD denotes 5
T18453 1474-1475 SYM denotes ′
T18454 1475-1476 : denotes -
T18455 1476-1479 NNP denotes GCT
T18456 1480-1483 NNP denotes CTC
T18457 1484-1487 NNP denotes CTG
T18458 1488-1491 NNP denotes GCT
T18459 1492-1495 NNP denotes GCA
T18460 1496-1499 NNP denotes GAC
T18461 1500-1505 JJ denotes AGC-3
T18462 1505-1506 NN denotes ′
T18463 1506-1507 . denotes .
T18464 1508-1511 DT denotes The
T18465 1512-1520 JJ denotes prepared
T18466 1521-1526 NNS denotes cDNAs
T18467 1527-1531 VBD denotes were
T18468 1532-1541 VBN denotes amplified
T18469 1542-1547 VBG denotes using
T18470 1548-1556 NNP denotes SYBR-PCR
T18471 1557-1566 NN denotes mastermix
T18472 1567-1568 -LRB- denotes (
T18473 1568-1574 NNP denotes Biorad
T18474 1574-1575 -RRB- denotes )
T18475 1576-1585 VBG denotes according
T18476 1586-1588 TO denotes to
T18477 1589-1592 DT denotes the
T18478 1593-1608 NNS denotes recommendations
T18479 1609-1611 IN denotes of
T18480 1612-1615 DT denotes the
T18481 1616-1628 NN denotes manufacturer
T18482 1629-1631 IN denotes in
T18483 1632-1634 DT denotes an
T18484 1635-1638 NNP denotes ABI
T18485 1639-1644 NNP denotes PRISM
T18486 1645-1649 CD denotes 7000
T18487 1650-1658 NNP denotes Sequence
T18488 1659-1668 NNP denotes Detection
T18489 1669-1675 NNP denotes System
T18490 1676-1677 -LRB- denotes (
T18491 1677-1684 NNP denotes Applied
T18492 1685-1695 NNPS denotes Biosystems
T18493 1695-1696 -RRB- denotes )
T18494 1696-1697 . denotes .
T18495 1698-1710 JJ denotes Quantitative
T18496 1711-1714 NNP denotes PCR
T18497 1715-1717 IN denotes of
T18498 1718-1724 NN denotes murine
T18499 1725-1732 NNS denotes samples
T18500 1733-1736 VBD denotes was
T18501 1737-1746 VBN denotes performed
T18502 1747-1751 IN denotes with
T18503 1752-1761 JJ denotes Brilliant
T18504 1762-1766 NNP denotes SYBR
T18505 1767-1772 NNP denotes Green
T18506 1773-1777 NNP denotes QPCR
T18507 1778-1784 NN denotes master
T18508 1785-1788 NN denotes mix
T18509 1789-1790 -LRB- denotes (
T18510 1790-1800 NNP denotes Stratagene
T18511 1800-1801 -RRB- denotes )
T18512 1802-1805 CC denotes and
T18513 1806-1809 DT denotes the
T18514 1810-1819 VBG denotes following
T18515 1820-1827 NNS denotes primers
T18516 1827-1828 : denotes :
T18517 1829-1838 NNP denotes Ubiquitin
T18518 1839-1840 NNP denotes C
T18519 1840-1841 , denotes ,
T18520 1842-1843 CD denotes 5
T18521 1843-1844 SYM denotes ′
T18522 1844-1845 : denotes -
T18523 1846-1849 NNP denotes AGG
T18524 1850-1853 NNP denotes TCA
T18525 1854-1857 NNP denotes AAC
T18526 1858-1861 NNP denotes AGG
T18527 1862-1865 NNP denotes AAG
T18528 1866-1869 NNP denotes ACA
T18529 1870-1873 NNP denotes GAC
T18530 1874-1879 NNP denotes GTA-3
T18531 1879-1880 NNP denotes ′
T18532 1881-1884 CC denotes and
T18533 1885-1886 CD denotes 5
T18534 1886-1887 SYM denotes ′
T18535 1887-1888 : denotes -
T18536 1888-1911 NN denotes TCACACCCAAGAACAAGCACA-3
T18537 1911-1912 NN denotes ′
T18538 1912-1913 : denotes ;
T18539 1914-1920 NNP denotes Smad-7
T18540 1920-1921 , denotes ,
T18541 1922-1923 CD denotes 5
T18542 1923-1924 SYM denotes ′
T18543 1924-1925 : denotes -
T18544 1925-1928 NNP denotes GAA
T18545 1929-1932 NNP denotes ACC
T18546 1933-1936 NNP denotes GGG
T18547 1937-1940 NNP denotes GGA
T18548 1941-1944 NNP denotes ACG
T18549 1945-1948 NNP denotes AAT
T18550 1949-1954 CD denotes TAT-3
T18551 1954-1955 NN denotes ′
T18552 1956-1959 CC denotes and
T18553 1960-1961 CD denotes 5
T18554 1961-1962 SYM denotes ′
T18555 1962-1963 : denotes -
T18556 1963-1966 NNP denotes CGC
T18557 1967-1970 NNP denotes GAG
T18558 1971-1974 NNP denotes TCT
T18559 1975-1978 NNP denotes TCT
T18560 1979-1982 NNP denotes CCT
T18561 1983-1986 NNP denotes CCC
T18562 1987-1990 JJ denotes A-3
T18563 1990-1991 NN denotes ′
T18564 1991-1992 : denotes ;
T18565 1993-1999 NNP denotes TGF-ß1
T18566 1999-2000 , denotes ,
T18567 2001-2002 CD denotes 5
T18568 2002-2003 SYM denotes ′
T18569 2003-2004 : denotes -
T18570 2004-2007 NNP denotes TGA
T18571 2008-2011 NNP denotes CGT
T18572 2012-2015 NNP denotes CAC
T18573 2016-2019 NNP denotes TGG
T18574 2020-2023 NNP denotes AGT
T18575 2024-2027 NNP denotes TGT
T18576 2028-2031 NNP denotes ACG
T18577 2032-2035 NNP denotes G-3
T18578 2035-2036 NNP denotes ′
T18579 2037-2040 CC denotes and
T18580 2041-2042 CD denotes 5
T18581 2042-2043 SYM denotes ′
T18582 2043-2044 : denotes -
T18583 2044-2047 NNP denotes GGT
T18584 2048-2051 NNP denotes TCA
T18585 2052-2055 NNP denotes TGT
T18586 2056-2059 NNP denotes CAT
T18587 2060-2063 NNP denotes GGA
T18588 2064-2067 NNP denotes TGG
T18589 2068-2073 CD denotes TGC-3
T18590 2073-2074 NN denotes ′
T18591 2074-2075 . denotes .
T18592 2076-2082 NNP denotes Primer
T18593 2083-2088 NNS denotes pairs
T18594 2089-2093 VBD denotes were
T18595 2094-2103 VBN denotes evaluated
T18596 2104-2107 IN denotes for
T18597 2108-2117 NN denotes integrity
T18598 2118-2120 IN denotes by
T18599 2121-2129 NN denotes analysis
T18600 2130-2132 IN denotes of
T18601 2133-2136 DT denotes the
T18602 2137-2150 NN denotes amplification
T18603 2151-2155 NN denotes plot
T18604 2155-2156 , denotes ,
T18605 2157-2169 NN denotes dissociation
T18606 2170-2176 NNS denotes curves
T18607 2176-2177 , denotes ,
T18608 2178-2181 CC denotes and
T18609 2182-2192 NN denotes efficiency
T18610 2193-2195 IN denotes of
T18611 2196-2199 NNP denotes PCR
T18612 2200-2213 NN denotes amplification
T18613 2213-2214 . denotes .
T18614 2215-2218 NNP denotes PCR
T18615 2219-2229 NNS denotes conditions
T18616 2230-2234 VBD denotes were
T18617 2235-2237 CD denotes 10
T18618 2238-2241 NN denotes min
T18619 2242-2244 IN denotes at
T18620 2245-2247 CD denotes 95
T18621 2248-2249 CD denotes °
T18622 2249-2250 NNP denotes C
T18623 2250-2251 , denotes ,
T18624 2252-2260 VBN denotes followed
T18625 2261-2263 IN denotes by
T18626 2264-2266 CD denotes 40
T18627 2267-2273 NNS denotes cycles
T18628 2274-2276 IN denotes of
T18629 2277-2279 CD denotes 15
T18630 2280-2281 PRP denotes s
T18631 2282-2284 IN denotes at
T18632 2285-2287 CD denotes 95
T18633 2288-2289 CD denotes °
T18634 2289-2290 NNP denotes C
T18635 2291-2294 CC denotes and
T18636 2295-2297 CD denotes 60
T18637 2298-2299 NN denotes °
T18638 2299-2300 NNP denotes C
T18639 2301-2304 IN denotes for
T18640 2305-2306 CD denotes 1
T18641 2307-2310 NN denotes min
T18642 2311-2316 VBG denotes using
T18643 2317-2319 DT denotes an
T18644 2320-2324 CD denotes 7300
T18645 2325-2334 JJ denotes real-time
T18646 2335-2338 NNP denotes PCR
T18647 2339-2345 NN denotes system
T18648 2346-2347 -LRB- denotes (
T18649 2347-2354 NNP denotes Applied
T18650 2355-2365 NNPS denotes Biosystems
T18651 2365-2366 -RRB- denotes )
T18652 2366-2367 . denotes .
T18653 2368-2371 NNP denotes PCR
T18654 2372-2385 NN denotes amplification
T18655 2386-2388 IN denotes of
T18656 2389-2392 DT denotes the
T18657 2393-2405 NN denotes housekeeping
T18658 2406-2410 NN denotes gene
T18659 2411-2419 VBG denotes encoding
T18660 2420-2429 NN denotes ubiquitin
T18661 2430-2431 NNP denotes C
T18662 2432-2435 VBD denotes was
T18663 2436-2445 VBN denotes performed
T18664 2446-2452 IN denotes during
T18665 2453-2457 DT denotes each
T18666 2458-2461 NN denotes run
T18667 2462-2465 IN denotes for
T18668 2466-2470 DT denotes each
T18669 2471-2477 NN denotes sample
T18670 2478-2480 TO denotes to
T18671 2481-2486 VB denotes allow
T18672 2487-2500 NN denotes normalization
T18673 2501-2508 IN denotes between
T18674 2509-2516 NNS denotes samples
T18675 2516-2517 . denotes .
T18676 2518-2526 JJ denotes Relative
T18677 2527-2541 NN denotes quantification
T18678 2542-2545 CC denotes and
T18679 2546-2557 NN denotes calculation
T18680 2558-2560 IN denotes of
T18681 2561-2564 DT denotes the
T18682 2565-2570 NN denotes range
T18683 2571-2573 IN denotes of
T18684 2574-2584 NN denotes confidence
T18685 2585-2588 VBD denotes was
T18686 2589-2598 VBN denotes performed
T18687 2599-2604 VBG denotes using
T18688 2605-2608 DT denotes the
T18689 2609-2620 JJ denotes comparative
T18690 2621-2625 NNP denotes ΔΔCT
T18691 2626-2632 NN denotes method
T18692 2632-2633 . denotes .
T21516 7730-7736 JJ denotes murine
T18896 2635-2644 JJ denotes Inducible
T18897 2645-2651 NN denotes murine
T18898 2652-2656 NNP denotes Treg
T18899 2657-2664 NN denotes culture
T18900 2664-2665 . denotes .
T18901 2666-2671 JJ denotes Naive
T18902 2672-2675 NNP denotes CD4
T18903 2675-2676 CD denotes +
T18904 2677-2678 NNP denotes T
T18905 2679-2684 NNS denotes cells
T18906 2685-2686 -LRB- denotes (
T18907 2686-2689 CD denotes CD4
T18908 2689-2690 NN denotes +
T18909 2690-2691 , denotes ,
T18910 2692-2697 NNP denotes CD62L
T18911 2697-2698 NNP denotes +
T18912 2698-2699 , denotes ,
T18913 2700-2703 CC denotes and
T18914 2704-2708 NNP denotes CD25
T18915 2709-2710 -RRB- denotes )
T18916 2711-2715 VBD denotes were
T18917 2716-2724 VBN denotes isolated
T18918 2725-2729 IN denotes from
T18919 2730-2736 VBN denotes pooled
T18920 2737-2742 NN denotes lymph
T18921 2743-2748 NNS denotes nodes
T18922 2749-2752 CC denotes and
T18923 2753-2760 NNS denotes spleens
T18924 2761-2763 IN denotes by
T18925 2764-2768 NNP denotes FACS
T18926 2769-2770 -LRB- denotes (
T18927 2770-2774 NNP denotes FACS
T18928 2775-2779 NNP denotes Aria
T18929 2779-2780 , denotes ,
T18930 2781-2783 NNP denotes BD
T18931 2784-2795 NNP denotes Biosciences
T18932 2795-2796 -RRB- denotes )
T18933 2796-2797 . denotes .
T18934 2798-2799 CD denotes 5
T18935 2800-2801 NN denotes ×
T18936 2802-2805 CD denotes 105
T18937 2806-2807 NNP denotes T
T18938 2808-2813 NNS denotes cells
T18939 2814-2818 VBD denotes were
T18940 2819-2830 VBN denotes co-cultured
T18941 2831-2835 IN denotes with
T18942 2836-2839 CD denotes 2.5
T18943 2840-2841 CD denotes ×
T18944 2842-2845 CD denotes 104
T18945 2846-2850 NN denotes bone
T18946 2851-2857 NN denotes marrow
T18947 2858-2865 VBN denotes derived
T18948 2866-2875 JJ denotes dendritic
T18949 2876-2881 NNS denotes cells
T18950 2882-2883 NNP denotes [
T18951 2883-2885 CD denotes 62
T18952 2885-2886 NNP denotes ]
T18953 2887-2890 CC denotes and
T18954 2891-2895 CD denotes 0.01
T18955 2896-2898 NN denotes μg
T18956 2898-2899 NN denotes /
T18957 2899-2901 NN denotes ml
T18958 2902-2908 NNP denotes OVA323
T18959 2909-2912 CD denotes 339
T18960 2913-2920 NN denotes peptide
T18961 2921-2922 -LRB- denotes (
T18962 2922-2929 NNP denotes Ansynth
T18963 2929-2930 -RRB- denotes )
T18964 2931-2933 IN denotes in
T18965 2934-2937 DT denotes the
T18966 2938-2946 NN denotes presence
T18967 2947-2949 CC denotes or
T18968 2950-2957 NN denotes absence
T18969 2958-2960 IN denotes of
T18970 2961-2966 JJ denotes 20-ng
T18971 2966-2967 NN denotes /
T18972 2967-2969 NN denotes μl
T18973 2970-2978 NN denotes rhTGF-ß1
T18974 2979-2980 -LRB- denotes (
T18975 2980-2989 NNP denotes Peprotech
T18976 2989-2990 -RRB- denotes )
T18977 2991-2993 IN denotes in
T18978 2994-3001 JJ denotes 48-well
T18979 3002-3008 NNS denotes plates
T18980 3008-3009 . denotes .
T18981 3010-3015 IN denotes After
T18982 3016-3017 CD denotes 4
T18983 3018-3019 LS denotes d
T18984 3019-3020 , denotes ,
T18985 3021-3026 NNS denotes cells
T18986 3027-3031 VBD denotes were
T18987 3032-3041 VBN denotes harvested
T18988 3042-3045 CC denotes and
T18989 3046-3054 VBN denotes analyzed
T18990 3055-3058 IN denotes for
T18991 3059-3072 JJ denotes intracellular
T18992 3073-3078 CD denotes FOXP3
T18993 3079-3089 NN denotes expression
T18994 3090-3092 IN denotes by
T18995 3093-3097 NNP denotes FACS
T18996 3098-3100 CC denotes or
T18997 3101-3105 NN denotes gene
T18998 3106-3116 NN denotes expression
T18999 3117-3119 IN denotes by
T19000 3120-3132 JJ denotes quantitative
T19001 3133-3139 NN denotes RT-PCR
T19002 3139-3140 . denotes .
T19255 3142-3144 IN denotes In
T19256 3145-3150 NN denotes vitro
T19257 3151-3152 NNP denotes T
T19258 3153-3157 NN denotes cell
T19259 3158-3173 NN denotes differentiation
T19260 3173-3174 . denotes .
T19261 3175-3178 CD denotes CD4
T19262 3178-3179 CD denotes +
T19263 3180-3186 NNP denotes CD45RA
T19264 3186-3187 NNP denotes +
T19265 3188-3207 JJ denotes magnetically-sorted
T19266 3208-3209 -LRB- denotes (
T19267 3209-3215 CD denotes CD45RO
T19268 3216-3225 NN denotes depletion
T19269 3225-3226 , denotes ,
T19270 3227-3231 NNP denotes MACS
T19271 3231-3232 , denotes ,
T19272 3233-3242 VBG denotes according
T19273 3243-3245 TO denotes to
T19274 3246-3249 DT denotes the
T19275 3250-3258 NN denotes protocol
T19276 3259-3261 IN denotes of
T19277 3262-3265 DT denotes the
T19278 3266-3278 NN denotes manufacturer
T19279 3278-3279 -RRB- denotes )
T19280 3280-3285 NNS denotes cells
T19281 3286-3290 VBD denotes were
T19282 3291-3301 VBN denotes stimulated
T19283 3302-3306 IN denotes with
T19284 3307-3318 JJ denotes immobilized
T19285 3319-3330 JJ denotes plate-bound
T19286 3331-3339 JJ denotes anti-CD3
T19287 3340-3341 -LRB- denotes (
T19288 3341-3342 CD denotes 1
T19289 3343-3345 NN denotes μg
T19290 3345-3346 NN denotes /
T19291 3346-3348 NN denotes ml
T19292 3348-3349 , denotes ,
T19293 3350-3354 NNP denotes Okt3
T19294 3354-3355 , denotes ,
T19295 3356-3360 NNP denotes IgG1
T19296 3360-3361 -RRB- denotes )
T19297 3362-3365 CC denotes and
T19298 3366-3375 JJ denotes anti-CD28
T19299 3376-3377 -LRB- denotes (
T19300 3377-3378 CD denotes 2
T19301 3379-3381 NN denotes μg
T19302 3381-3382 NN denotes /
T19303 3382-3384 NN denotes ml
T19304 3384-3385 -RRB- denotes )
T19305 3386-3388 IN denotes in
T19306 3389-3392 JJ denotes Th1
T19307 3393-3403 NNS denotes conditions
T19308 3403-3404 : denotes :
T19309 3405-3407 CD denotes 25
T19310 3408-3413 NN denotes ng/ml
T19311 3414-3419 NN denotes IL-12
T19312 3419-3420 , denotes ,
T19313 3421-3422 CD denotes 5
T19314 3423-3425 NN denotes μg
T19315 3425-3426 NN denotes /
T19316 3426-3428 NN denotes ml
T19317 3429-3438 NN denotes anti-IL-4
T19318 3439-3440 -LRB- denotes (
T19319 3440-3443 NNP denotes R&D
T19320 3444-3451 NNS denotes systems
T19321 3451-3452 -RRB- denotes )
T19322 3452-3453 : denotes ;
T19323 3454-3456 IN denotes in
T19324 3457-3460 JJ denotes Th2
T19325 3461-3471 NNS denotes conditions
T19326 3471-3472 : denotes :
T19327 3473-3475 CD denotes 25
T19328 3476-3481 NN denotes ng/ml
T19329 3482-3486 NN denotes IL-4
T19330 3486-3487 , denotes ,
T19331 3488-3489 CD denotes 5
T19332 3490-3492 NN denotes μg
T19333 3492-3493 NN denotes /
T19334 3493-3495 NN denotes ml
T19335 3496-3506 JJ denotes anti-IFN-γ
T19336 3506-3507 , denotes ,
T19337 3508-3509 CD denotes 5
T19338 3510-3512 NN denotes μg
T19339 3512-3513 NN denotes /
T19340 3513-3515 NN denotes ml
T19341 3516-3526 NN denotes anti-IL-12
T19342 3527-3528 -LRB- denotes (
T19343 3528-3531 NNP denotes R&D
T19344 3532-3539 NNS denotes systems
T19345 3539-3540 -RRB- denotes )
T19346 3540-3541 : denotes ;
T19347 3542-3544 CC denotes or
T19348 3545-3547 IN denotes in
T19349 3548-3552 NNP denotes Treg
T19350 3553-3563 NNS denotes conditions
T19351 3563-3564 : denotes :
T19352 3565-3567 CD denotes 10
T19353 3568-3573 NN denotes ng/ml
T19354 3574-3579 NNP denotes TGF-β
T19355 3579-3580 , denotes ,
T19356 3581-3582 CD denotes 5
T19357 3583-3585 NN denotes μg
T19358 3585-3586 NN denotes /
T19359 3586-3588 NN denotes ml
T19360 3589-3599 JJ denotes anti-IFN-γ
T19361 3599-3600 , denotes ,
T19362 3601-3602 CD denotes 5
T19363 3603-3605 NN denotes μg
T19364 3605-3606 NN denotes /
T19365 3606-3608 NN denotes ml
T19366 3609-3619 NN denotes anti-IL-12
T19367 3619-3620 , denotes ,
T19368 3621-3622 CD denotes 5
T19369 3623-3625 NN denotes μg
T19370 3625-3626 NN denotes /
T19371 3626-3628 NN denotes ml
T19372 3629-3638 NN denotes anti-IL-4
T19373 3638-3639 . denotes .
T19374 3640-3653 VBG denotes Proliferating
T19375 3654-3659 NNS denotes cells
T19376 3660-3664 VBD denotes were
T19377 3665-3673 VBN denotes expanded
T19378 3674-3676 IN denotes in
T19379 3677-3683 JJ denotes medium
T19380 3684-3694 VBG denotes containing
T19381 3695-3699 NN denotes IL-2
T19382 3700-3701 -LRB- denotes (
T19383 3701-3703 CD denotes 30
T19384 3704-3709 NN denotes ng/ml
T19385 3709-3710 -RRB- denotes )
T19386 3710-3711 . denotes .
T19618 3713-3720 VBG denotes Cloning
T19619 3721-3723 IN denotes of
T19620 3724-3727 DT denotes the
T19621 3728-3733 CD denotes FOXP3
T19622 3734-3742 NN denotes promoter
T19623 3743-3746 CC denotes and
T19624 3747-3759 NN denotes construction
T19625 3760-3762 IN denotes of
T19626 3763-3769 JJ denotes mutant
T19627 3770-3780 NNS denotes constructs
T19628 3780-3781 . denotes .
T19629 3782-3785 DT denotes The
T19630 3786-3791 CD denotes FOXP3
T19631 3792-3800 NN denotes promoter
T19632 3801-3804 VBD denotes was
T19633 3805-3811 VBN denotes cloned
T19634 3812-3816 IN denotes into
T19635 3817-3820 DT denotes the
T19636 3821-3825 JJ denotes pGL3
T19637 3826-3831 JJ denotes basic
T19638 3832-3838 NN denotes vector
T19639 3839-3840 -LRB- denotes (
T19640 3840-3847 NNP denotes Promega
T19641 3848-3855 NNP denotes Biotech
T19642 3855-3856 -RRB- denotes )
T19643 3857-3859 TO denotes to
T19644 3860-3868 VB denotes generate
T19645 3869-3872 DT denotes the
T19646 3873-3877 JJ denotes pGL3
T19647 3878-3883 CD denotes FOXP3
T19648 3884-3885 CD denotes −
T19649 3885-3888 CD denotes 511
T19650 3888-3889 NN denotes /
T19651 3889-3893 CD denotes +176
T19652 3894-3895 NNP denotes [
T19653 3895-3897 CD denotes 24
T19654 3897-3898 NNP denotes ]
T19655 3898-3899 . denotes .
T19656 3900-3913 JJ denotes Site-directed
T19657 3914-3925 NNS denotes mutagenesis
T19658 3926-3928 IN denotes in
T19659 3929-3932 DT denotes the
T19660 3933-3938 CD denotes FOXP3
T19661 3939-3947 NN denotes promoter
T19662 3948-3954 NN denotes region
T19663 3955-3959 VBD denotes were
T19664 3960-3970 VBN denotes introduced
T19665 3971-3976 VBG denotes using
T19666 3977-3980 DT denotes the
T19667 3981-3992 NNP denotes QuickChange
T19668 3993-3996 NN denotes kit
T19669 3997-3998 -LRB- denotes (
T19670 3998-4008 NNP denotes Stratagene
T19671 4008-4009 -RRB- denotes )
T19672 4009-4010 , denotes ,
T19673 4011-4020 VBG denotes according
T19674 4021-4023 TO denotes to
T19675 4024-4027 DT denotes the
T19676 4028-4040 NN denotes manufacturer
T19677 4040-4042 POS denotes 's
T19678 4043-4055 NNS denotes instructions
T19679 4055-4056 . denotes .
T19680 4057-4060 DT denotes The
T19681 4061-4070 JJ denotes following
T19682 4071-4077 NN denotes primer
T19683 4078-4081 CC denotes and
T19684 4082-4085 PRP$ denotes its
T19685 4086-4099 JJ denotes complementary
T19686 4100-4106 NN denotes strand
T19687 4107-4111 VBD denotes were
T19688 4112-4116 VBN denotes used
T19689 4116-4117 : denotes :
T19690 4118-4121 NNP denotes GTT
T19691 4122-4125 NNP denotes TCT
T19692 4126-4129 NNP denotes CAT
T19693 4130-4133 NNP denotes GAG
T19694 4134-4137 NNP denotes CCC
T19695 4138-4141 NNP denotes TAT
T19696 4142-4145 NNP denotes TAA
T19697 4146-4149 NNP denotes GTC
T19698 4150-4153 NNP denotes ATT
T19699 4154-4157 NNP denotes CTT
T19700 4158-4161 NNP denotes ACC
T19701 4162-4165 NNP denotes TCT
T19702 4166-4169 NNP denotes CAC
T19703 4170-4173 NNP denotes CTC
T19704 4174-4177 NNP denotes TGT
T19705 4178-4181 NNP denotes GGT
T19706 4182-4185 NNP denotes GA.
T19885 4187-4200 NNS denotes Transfections
T19886 4201-4204 CC denotes and
T19887 4205-4213 NN denotes reporter
T19888 4214-4218 NN denotes gene
T19889 4219-4225 NNS denotes assays
T19890 4225-4226 . denotes .
T19891 4227-4228 NNP denotes T
T19892 4229-4234 NNS denotes cells
T19893 4235-4239 VBD denotes were
T19894 4240-4246 VBN denotes rested
T19895 4247-4249 IN denotes in
T19896 4250-4260 JJ denotes serum-free
T19897 4261-4266 NNP denotes AIM-V
T19898 4267-4273 NN denotes medium
T19899 4274-4275 -LRB- denotes (
T19900 4275-4279 NNP denotes Life
T19901 4280-4292 NNPS denotes Technologies
T19902 4292-4293 -RRB- denotes )
T19903 4294-4303 RB denotes overnight
T19904 4303-4304 . denotes .
T19905 4305-4308 CD denotes 3.5
T19906 4309-4311 NN denotes μg
T19907 4312-4314 IN denotes of
T19908 4315-4318 DT denotes the
T19909 4319-4324 CD denotes FOXP3
T19910 4325-4333 NN denotes promoter
T19911 4334-4344 NN denotes luciferase
T19912 4345-4353 NN denotes reporter
T19913 4354-4360 NN denotes vector
T19914 4361-4364 CC denotes and
T19915 4365-4368 CD denotes 0.5
T19916 4369-4371 NN denotes μg
T19917 4372-4379 NN denotes phRL-TK
T19918 4380-4384 VBD denotes were
T19919 4385-4390 VBN denotes added
T19920 4391-4393 TO denotes to
T19921 4394-4395 CD denotes 3
T19922 4396-4397 CD denotes ×
T19923 4398-4401 CD denotes 106
T19924 4402-4405 NNP denotes CD4
T19925 4405-4406 CD denotes +
T19926 4407-4408 NNP denotes T
T19927 4409-4414 NNS denotes cells
T19928 4415-4426 VBD denotes resuspended
T19929 4427-4429 IN denotes in
T19930 4430-4433 CD denotes 100
T19931 4434-4436 NN denotes μL
T19932 4437-4439 IN denotes of
T19933 4440-4452 NNP denotes Nucleofector
T19934 4453-4461 NN denotes solution
T19935 4462-4463 -LRB- denotes (
T19936 4463-4468 NNP denotes Amaxa
T19937 4469-4479 NNPS denotes Biosystems
T19938 4479-4480 -RRB- denotes )
T19939 4481-4484 CC denotes and
T19940 4485-4499 VBD denotes electroporated
T19941 4500-4505 VBG denotes using
T19942 4506-4509 DT denotes the
T19943 4510-4514 NN denotes U-15
T19944 4515-4522 NN denotes program
T19945 4523-4525 IN denotes of
T19946 4526-4529 DT denotes the
T19947 4530-4542 NNP denotes Nucleofector
T19948 4542-4543 . denotes .
T19949 4544-4549 IN denotes After
T19950 4550-4551 DT denotes a
T19951 4552-4556 JJ denotes 24-h
T19952 4557-4564 NN denotes culture
T19953 4565-4567 IN denotes in
T19954 4568-4578 JJ denotes serum-free
T19955 4579-4589 NNS denotes conditions
T19956 4590-4593 CC denotes and
T19957 4594-4601 NNS denotes stimuli
T19958 4602-4604 IN denotes as
T19959 4605-4614 VBN denotes indicated
T19960 4615-4617 IN denotes in
T19961 4618-4621 DT denotes the
T19962 4622-4629 NNS denotes figures
T19963 4629-4630 , denotes ,
T19964 4631-4641 NN denotes luciferase
T19965 4642-4650 NN denotes activity
T19966 4651-4654 VBD denotes was
T19967 4655-4663 VBN denotes measured
T19968 4664-4666 IN denotes by
T19969 4667-4670 DT denotes the
T19970 4671-4675 JJ denotes dual
T19971 4676-4686 NN denotes luciferase
T19972 4687-4692 NN denotes assay
T19973 4693-4699 NN denotes system
T19974 4700-4701 -LRB- denotes (
T19975 4701-4708 NNP denotes Promega
T19976 4709-4716 NNP denotes Biotech
T19977 4716-4717 -RRB- denotes )
T19978 4718-4727 VBG denotes according
T19979 4728-4730 TO denotes to
T19980 4731-4734 DT denotes the
T19981 4735-4747 NN denotes manufacturer
T19982 4747-4749 POS denotes 's
T19983 4750-4762 NNS denotes instructions
T19984 4762-4763 . denotes .
T19985 4764-4768 NNS denotes Data
T19986 4769-4773 VBD denotes were
T19987 4774-4784 VBN denotes normalized
T19988 4785-4787 IN denotes by
T19989 4788-4791 DT denotes the
T19990 4792-4800 NN denotes activity
T19991 4801-4803 IN denotes of
T19992 4804-4811 NN denotes renilla
T19993 4812-4822 NN denotes luciferase
T19994 4822-4823 . denotes .
T20100 4825-4828 NNP denotes RNA
T20101 4829-4838 NN denotes isolation
T20102 4839-4842 CC denotes and
T20103 4843-4847 NNP denotes cDNA
T20104 4848-4857 NN denotes synthesis
T20105 4857-4858 . denotes .
T20106 4859-4862 NNP denotes RNA
T20107 4863-4866 VBD denotes was
T20108 4867-4875 VBN denotes isolated
T20109 4876-4881 VBG denotes using
T20110 4882-4885 DT denotes the
T20111 4886-4892 NNP denotes RNeasy
T20112 4893-4897 NNP denotes Mini
T20113 4898-4901 NNP denotes Kit
T20114 4902-4903 -LRB- denotes (
T20115 4903-4909 NNP denotes Qiagen
T20116 4909-4910 -RRB- denotes )
T20117 4911-4920 VBG denotes according
T20118 4921-4923 TO denotes to
T20119 4924-4927 DT denotes the
T20120 4928-4940 NN denotes manufacturer
T20121 4940-4942 POS denotes 's
T20122 4943-4951 NN denotes protocol
T20123 4951-4952 . denotes .
T20124 4953-4960 VB denotes Reverse
T20125 4961-4974 NN denotes transcription
T20126 4975-4977 IN denotes of
T20127 4978-4983 JJ denotes human
T20128 4984-4991 NNS denotes samples
T20129 4992-4995 VBD denotes was
T20130 4996-5005 VBN denotes performed
T20131 5006-5010 IN denotes with
T20132 5011-5017 NNP denotes TaqMan
T20133 5018-5039 NN denotes reverse-transcription
T20134 5040-5048 NNS denotes reagents
T20135 5049-5050 -LRB- denotes (
T20136 5050-5057 NNP denotes Applied
T20137 5058-5068 NNPS denotes Biosystems
T20138 5068-5069 -RRB- denotes )
T20139 5070-5074 IN denotes with
T20140 5075-5081 JJ denotes random
T20141 5082-5090 NNS denotes hexamers
T20142 5091-5100 VBG denotes according
T20143 5101-5103 TO denotes to
T20144 5104-5107 DT denotes the
T20145 5108-5120 NN denotes manufacturer
T20146 5120-5122 POS denotes 's
T20147 5123-5131 NN denotes protocol
T20148 5131-5132 . denotes .
T20272 5134-5145 JJ denotes Recombinant
T20273 5146-5149 NNP denotes TAT
T20274 5150-5158 NNS denotes proteins
T20275 5158-5159 . denotes .
T20276 5160-5163 DT denotes The
T20277 5164-5169 NNP denotes cDNAs
T20278 5170-5178 VBG denotes encoding
T20279 5179-5184 CD denotes GATA3
T20280 5185-5192 NN denotes protein
T20281 5193-5195 CC denotes or
T20282 5196-5199 DT denotes the
T20283 5200-5209 VBN denotes truncated
T20284 5210-5215 NNP denotes GATA3
T20285 5216-5217 -LRB- denotes (
T20286 5217-5224 VBG denotes lacking
T20287 5225-5228 DT denotes the
T20288 5229-5232 CD denotes two
T20289 5233-5237 NN denotes zinc
T20290 5238-5245 NNS denotes fingers
T20291 5245-5246 -RRB- denotes )
T20292 5247-5251 VBD denotes were
T20293 5252-5258 VBN denotes cloned
T20294 5259-5261 IN denotes in
T20295 5262-5267 NN denotes frame
T20296 5268-5272 IN denotes into
T20297 5273-5275 DT denotes an
T20298 5276-5286 NN denotes expression
T20299 5287-5293 NN denotes vector
T20300 5294-5299 IN denotes along
T20301 5300-5304 IN denotes with
T20302 5305-5308 DT denotes the
T20303 5309-5312 NNP denotes TAT
T20304 5313-5321 NN denotes sequence
T20305 5322-5324 IN denotes as
T20306 5325-5335 RB denotes previously
T20307 5336-5345 VBN denotes described
T20308 5346-5347 NNP denotes [
T20309 5347-5349 CD denotes 63
T20310 5349-5350 NNP denotes ]
T20311 5350-5351 . denotes .
T20312 5352-5360 NNPS denotes Proteins
T20313 5361-5365 VBD denotes were
T20314 5366-5375 VBN denotes expressed
T20315 5376-5378 IN denotes in
T20316 5379-5383 NNP denotes BL21
T20317 5384-5388 NNP denotes Star
T20318 5389-5390 -LRB- denotes (
T20319 5390-5393 NNP denotes DE3
T20320 5393-5394 -RRB- denotes )
T20321 5394-5399 NNP denotes pLysS
T20322 5400-5401 -LRB- denotes (
T20323 5401-5411 NNP denotes Invitrogen
T20324 5411-5412 -RRB- denotes )
T20325 5413-5416 CC denotes and
T20326 5417-5424 NNS denotes lysates
T20327 5425-5429 VBD denotes were
T20328 5430-5438 VBN denotes purified
T20329 5439-5441 IN denotes by
T20330 5442-5445 NNP denotes Ni2
T20331 5445-5446 SYM denotes +
T20332 5446-5447 : denotes -
T20333 5447-5454 NN denotes chelate
T20334 5455-5461 NN denotes column
T20335 5462-5476 NN denotes chromatography
T20336 5476-5477 . denotes .
T20337 5478-5482 DT denotes Both
T20338 5483-5493 JJ denotes TAT-linked
T20339 5494-5502 NNS denotes proteins
T20340 5503-5507 VBD denotes were
T20341 5508-5512 JJR denotes more
T20342 5513-5517 IN denotes than
T20343 5518-5520 CD denotes 95
T20344 5520-5521 NN denotes %
T20345 5522-5526 JJ denotes pure
T20346 5526-5527 , denotes ,
T20347 5528-5533 VBN denotes based
T20348 5534-5536 IN denotes on
T20349 5537-5546 NNP denotes Coomassie
T20350 5547-5551 JJ denotes blue
T20351 5552-5560 NN denotes staining
T20352 5561-5563 IN denotes of
T20353 5564-5570 NN denotes sodium
T20354 5571-5580 NN denotes disulfate
T20355 5581-5591 NN denotes acrylamide
T20356 5592-5596 NNS denotes gels
T20357 5596-5597 . denotes .
T20544 5599-5608 CD denotes TAT-GATA3
T20545 5609-5621 NN denotes transduction
T20546 5621-5622 . denotes .
T20547 5623-5626 CD denotes CD4
T20548 5626-5627 CD denotes +
T20549 5627-5633 NNP denotes CD45RA
T20550 5633-5634 NN denotes +
T20551 5635-5640 NNS denotes cells
T20552 5641-5645 VBD denotes were
T20553 5646-5654 VBN denotes cultured
T20554 5655-5657 IN denotes in
T20555 5658-5662 NNP denotes AIMV
T20556 5663-5669 NN denotes medium
T20557 5670-5673 CC denotes and
T20558 5674-5684 VBN denotes transduced
T20559 5685-5689 IN denotes with
T20560 5690-5692 CD denotes 20
T20561 5693-5695 NNP denotes nM
T20562 5695-5696 , denotes ,
T20563 5697-5699 CD denotes 10
T20564 5700-5702 NN denotes nM
T20565 5702-5703 , denotes ,
T20566 5704-5706 CC denotes or
T20567 5707-5710 CD denotes 500
T20568 5711-5713 NN denotes nM
T20569 5714-5716 IN denotes of
T20570 5717-5728 JJ denotes full-length
T20571 5729-5731 CC denotes or
T20572 5732-5741 VBN denotes truncated
T20573 5742-5747 NNP denotes GATA3
T20574 5748-5752 IN denotes over
T20575 5753-5756 DT denotes the
T20576 5757-5763 NN denotes course
T20577 5764-5766 IN denotes of
T20578 5767-5768 CD denotes 4
T20579 5769-5770 NN denotes h
T20580 5770-5771 . denotes .
T20581 5772-5777 IN denotes After
T20582 5778-5779 CD denotes 4
T20583 5780-5781 NN denotes h
T20584 5781-5782 , denotes ,
T20585 5783-5786 DT denotes the
T20586 5787-5792 NNS denotes cells
T20587 5793-5797 VBD denotes were
T20588 5798-5804 VBN denotes washed
T20589 5805-5808 CC denotes and
T20590 5809-5818 VBN denotes activated
T20591 5819-5823 IN denotes with
T20592 5824-5831 JJ denotes soluble
T20593 5832-5840 JJ denotes anti-CD3
T20594 5841-5844 CC denotes and
T20595 5845-5854 JJ denotes anti-CD28
T20596 5855-5858 CC denotes and
T20597 5859-5864 JJ denotes TGF-β
T20598 5865-5866 -LRB- denotes (
T20599 5866-5868 CD denotes 10
T20600 5869-5874 NN denotes ng/ml
T20601 5874-5875 -RRB- denotes )
T20602 5875-5876 . denotes .
T20603 5877-5881 DT denotes Each
T20604 5882-5885 NN denotes day
T20605 5885-5886 , denotes ,
T20606 5887-5890 DT denotes the
T20607 5891-5894 NNP denotes TAT
T20608 5895-5903 NNS denotes proteins
T20609 5904-5908 VBD denotes were
T20610 5909-5916 RB denotes freshly
T20611 5917-5922 VBN denotes added
T20612 5923-5925 TO denotes to
T20613 5926-5929 DT denotes the
T20614 5930-5936 NN denotes medium
T20615 5936-5937 . denotes .
T20616 5938-5943 CD denotes FOXP3
T20617 5944-5954 NN denotes expression
T20618 5955-5958 VBD denotes was
T20619 5959-5967 VBN denotes measured
T20620 5968-5973 IN denotes after
T20621 5974-5975 CD denotes 5
T20622 5976-5977 LS denotes d
T20623 5978-5980 IN denotes by
T20624 5981-5994 JJ denotes intracellular
T20625 5995-6003 NN denotes staining
T20626 6003-6004 . denotes .
T20794 6006-6019 JJ denotes Intracellular
T20795 6020-6028 NN denotes cytokine
T20796 6029-6037 NN denotes staining
T20797 6037-6038 . denotes .
T20798 6039-6040 NNP denotes T
T20799 6041-6046 NNS denotes cells
T20800 6047-6051 VBD denotes were
T20801 6052-6062 VBN denotes stimulated
T20802 6063-6067 IN denotes with
T20803 6068-6069 CD denotes 2
T20804 6070-6071 CD denotes ×
T20805 6072-6074 CD denotes 10
T20806 6074-6075 NN denotes −
T20807 6075-6076 CD denotes 7
T20808 6077-6078 NNP denotes M
T20809 6079-6082 NNP denotes PMA
T20810 6083-6086 CC denotes and
T20811 6087-6088 CD denotes 1
T20812 6089-6091 NN denotes μg
T20813 6091-6092 NN denotes /
T20814 6092-6094 NN denotes ml
T20815 6095-6097 IN denotes of
T20816 6098-6107 NN denotes ionomycin
T20817 6108-6109 -LRB- denotes (
T20818 6109-6114 NNP denotes Sigma
T20819 6115-6124 NNPS denotes Chemicals
T20820 6124-6125 -RRB- denotes )
T20821 6126-6129 IN denotes for
T20822 6130-6131 CD denotes 4
T20823 6132-6133 NN denotes h
T20824 6133-6134 . denotes .
T20825 6135-6138 DT denotes The
T20826 6139-6148 JJ denotes following
T20827 6149-6152 NNP denotes mAb
T20828 6153-6156 VBD denotes was
T20829 6157-6161 VBN denotes used
T20830 6161-6162 : denotes :
T20831 6163-6175 JJ denotes anti-IL-4-PE
T20832 6176-6177 -LRB- denotes (
T20833 6177-6180 CD denotes 8D4
T20834 6181-6182 CD denotes 8
T20835 6182-6183 , denotes ,
T20836 6184-6186 NNP denotes BD
T20837 6186-6187 -RRB- denotes )
T20838 6187-6188 . denotes .
T20839 6189-6196 VBN denotes Matched
T20840 6197-6204 NN denotes isotype
T20841 6205-6213 NNS denotes controls
T20842 6214-6218 VBD denotes were
T20843 6219-6223 VBN denotes used
T20844 6224-6226 IN denotes at
T20845 6227-6230 DT denotes the
T20846 6231-6235 JJ denotes same
T20847 6236-6243 NN denotes protein
T20848 6244-6257 NN denotes concentration
T20849 6258-6260 IN denotes as
T20850 6261-6264 DT denotes the
T20851 6265-6275 JJ denotes respective
T20852 6276-6286 NNS denotes antibodies
T20853 6286-6287 . denotes .
T20854 6288-6298 JJ denotes Four-color
T20855 6299-6303 NNP denotes FACS
T20856 6304-6307 VBD denotes was
T20857 6308-6317 VBN denotes performed
T20858 6318-6323 VBG denotes using
T20859 6324-6326 DT denotes an
T20860 6327-6332 NNP denotes EPICS
T20861 6333-6339 NNP denotes XL-MCL
T20862 6340-6341 -LRB- denotes (
T20863 6341-6348 NNP denotes Beckman
T20864 6349-6356 NNP denotes Coulter
T20865 6356-6357 -RRB- denotes )
T20866 6358-6363 VBG denotes using
T20867 6364-6367 DT denotes the
T20868 6368-6376 NN denotes software
T20869 6377-6383 NNP denotes Expo32
T20870 6384-6391 NN denotes version
T20871 6392-6395 IN denotes for
T20872 6396-6400 NNS denotes data
T20873 6401-6412 NN denotes acquisition
T20874 6413-6416 CC denotes and
T20875 6417-6427 NN denotes evaluation
T20876 6427-6428 . denotes .
T21273 6430-6434 NN denotes Flow
T21274 6435-6444 NN denotes cytometry
T21275 6444-6445 . denotes .
T21276 6446-6449 IN denotes For
T21277 6450-6458 NN denotes analysis
T21278 6459-6461 IN denotes of
T21279 6462-6467 CD denotes FOXP3
T21280 6468-6478 NN denotes expression
T21281 6479-6481 IN denotes at
T21282 6482-6485 DT denotes the
T21283 6486-6497 JJ denotes single-cell
T21284 6498-6503 NN denotes level
T21285 6503-6504 , denotes ,
T21286 6505-6510 NNS denotes cells
T21287 6511-6515 VBD denotes were
T21288 6516-6521 JJ denotes first
T21289 6522-6529 VBN denotes stained
T21290 6530-6534 IN denotes with
T21291 6535-6538 DT denotes the
T21292 6539-6549 JJ denotes monoclonal
T21293 6550-6558 NN denotes antibody
T21294 6559-6563 NNS denotes CD25
T21295 6564-6565 -LRB- denotes (
T21296 6565-6572 NNP denotes Beckman
T21297 6573-6580 NNP denotes Coulter
T21298 6580-6581 -RRB- denotes )
T21299 6581-6582 , denotes ,
T21300 6583-6586 CC denotes and
T21301 6587-6592 IN denotes after
T21302 6593-6601 NN denotes fixation
T21303 6602-6605 CC denotes and
T21304 6606-6622 NN denotes permeabilization
T21305 6622-6623 , denotes ,
T21306 6624-6629 NNS denotes cells
T21307 6630-6634 VBD denotes were
T21308 6635-6644 VBN denotes incubated
T21309 6645-6649 IN denotes with
T21310 6650-6663 JJ denotes PE-conjugated
T21311 6664-6674 JJ denotes monoclonal
T21312 6675-6683 NN denotes antibody
T21313 6684-6690 NNP denotes PCH101
T21314 6691-6692 -LRB- denotes (
T21315 6692-6702 JJ denotes anti-human
T21316 6703-6708 CD denotes FOXP3
T21317 6708-6709 : denotes ;
T21318 6710-6721 NNP denotes eBioscience
T21319 6721-6722 -RRB- denotes )
T21320 6723-6728 VBN denotes based
T21321 6729-6731 IN denotes on
T21322 6732-6735 DT denotes the
T21323 6736-6748 NN denotes manufacturer
T21324 6748-6750 POS denotes 's
T21325 6751-6766 NNS denotes recommendations
T21326 6767-6770 CC denotes and
T21327 6771-6780 VBN denotes subjected
T21328 6781-6783 TO denotes to
T21329 6784-6788 NNP denotes FACS
T21330 6789-6790 -LRB- denotes (
T21331 6790-6795 NNP denotes EPICS
T21332 6796-6802 NNP denotes XL-MCL
T21333 6802-6803 -RRB- denotes )
T21334 6803-6804 . denotes .
T21335 6805-6808 IN denotes For
T21336 6809-6813 NN denotes cell
T21337 6814-6821 NN denotes surface
T21338 6822-6828 NN denotes marker
T21339 6829-6837 NN denotes staining
T21340 6837-6838 , denotes ,
T21341 6839-6844 NNS denotes cells
T21342 6845-6849 VBD denotes were
T21343 6850-6859 VBN denotes incubated
T21344 6860-6863 IN denotes for
T21345 6864-6866 CD denotes 20
T21346 6867-6870 NN denotes min
T21347 6871-6873 IN denotes at
T21348 6874-6875 CD denotes 4
T21349 6875-6876 CD denotes °
T21350 6876-6877 NNP denotes C
T21351 6878-6880 IN denotes in
T21352 6881-6889 VBG denotes staining
T21353 6890-6896 NN denotes buffer
T21354 6897-6901 IN denotes with
T21355 6902-6905 DT denotes the
T21356 6906-6915 VBG denotes following
T21357 6916-6926 NNS denotes antibodies
T21358 6926-6927 : denotes :
T21359 6928-6932 NNS denotes anti
T21360 6933-6941 NN denotes CD152-PE
T21361 6942-6943 -LRB- denotes (
T21362 6943-6949 NN denotes CTLA-4
T21363 6949-6950 : denotes ;
T21364 6951-6953 NNP denotes BD
T21365 6953-6954 -RRB- denotes )
T21366 6954-6955 , denotes ,
T21367 6956-6960 NNS denotes anti
T21368 6961-6965 NN denotes PD-1
T21369 6966-6967 -LRB- denotes (
T21370 6967-6979 NNS denotes eBiosciences
T21371 6979-6980 -RRB- denotes )
T21372 6980-6981 , denotes ,
T21373 6982-6991 JJ denotes anti-GITR
T21374 6992-6993 -LRB- denotes (
T21375 6993-6994 NN denotes R
T21376 6995-6996 CC denotes &
T21377 6997-6998 NNP denotes D
T21378 6999-7006 NNPS denotes Systems
T21379 7006-7007 -RRB- denotes )
T21380 7007-7008 , denotes ,
T21381 7009-7018 JJ denotes anti-CD69
T21382 7019-7020 -LRB- denotes (
T21383 7020-7027 NNP denotes Beckman
T21384 7028-7035 NNP denotes Coulter
T21385 7035-7036 -RRB- denotes )
T21386 7036-7037 , denotes ,
T21387 7038-7048 JJ denotes anti-CD103
T21388 7049-7050 -LRB- denotes (
T21389 7050-7064 NNP denotes DakoCytomation
T21390 7064-7065 -RRB- denotes )
T21391 7065-7066 , denotes ,
T21392 7067-7077 JJ denotes anti-CD62L
T21393 7078-7079 -LRB- denotes (
T21394 7079-7086 NNP denotes Beckman
T21395 7087-7094 NNP denotes Coulter
T21396 7094-7095 -RRB- denotes )
T21397 7095-7096 , denotes ,
T21398 7097-7099 CC denotes or
T21399 7100-7111 JJ denotes anti-HLA-DR
T21400 7112-7113 -LRB- denotes (
T21401 7113-7120 NNP denotes Beckman
T21402 7121-7128 NNP denotes Coulter
T21403 7128-7129 -RRB- denotes )
T21404 7129-7130 . denotes .
T21405 7131-7134 DT denotes The
T21406 7135-7143 NNS denotes controls
T21407 7144-7148 VBD denotes were
T21408 7149-7153 NNP denotes FITC
T21409 7153-7154 , denotes ,
T21410 7155-7157 NNP denotes PE
T21411 7157-7158 , denotes ,
T21412 7159-7161 CC denotes or
T21413 7162-7176 JJ denotes ECD-conjugated
T21414 7177-7182 NN denotes mouse
T21415 7183-7187 NN denotes IgG1
T21416 7188-7190 CC denotes or
T21417 7191-7194 NN denotes rat
T21418 7195-7200 NNS denotes IgG2a
T21419 7200-7201 . denotes .
T21420 7202-7205 IN denotes For
T21421 7206-7214 VBG denotes staining
T21422 7215-7217 IN denotes of
T21423 7218-7223 NN denotes mouse
T21424 7224-7229 NNS denotes cells
T21425 7229-7230 , denotes ,
T21426 7231-7234 DT denotes the
T21427 7235-7244 VBG denotes following
T21428 7245-7249 NNS denotes mAbs
T21429 7250-7254 IN denotes from
T21430 7255-7257 NNP denotes BD
T21431 7258-7269 NNPS denotes Biosciences
T21432 7270-7274 VBD denotes were
T21433 7275-7279 VBN denotes used
T21434 7280-7289 JJ denotes following
T21435 7290-7298 NN denotes standard
T21436 7299-7309 NNS denotes techniques
T21437 7310-7312 IN denotes as
T21438 7313-7322 VBN denotes described
T21439 7323-7328 IN denotes above
T21440 7328-7329 : denotes :
T21441 7330-7338 JJ denotes anti-CD3
T21442 7338-7339 , denotes ,
T21443 7340-7348 JJ denotes anti-CD4
T21444 7348-7349 , denotes ,
T21445 7350-7353 CC denotes and
T21446 7354-7363 NN denotes anti-CD25
T21447 7363-7364 . denotes .
T21448 7365-7376 NNP denotes Anti-FcγRII
T21449 7376-7377 NNP denotes /
T21450 7377-7380 NNP denotes III
T21451 7381-7389 NN denotes antibody
T21452 7390-7391 -LRB- denotes (
T21453 7391-7394 CD denotes 2.4
T21454 7394-7396 NNP denotes G2
T21455 7396-7397 , denotes ,
T21456 7398-7402 NNP denotes ATCC
T21457 7402-7403 -RRB- denotes )
T21458 7404-7407 VBD denotes was
T21459 7408-7416 VBN denotes included
T21460 7417-7419 IN denotes in
T21461 7420-7423 DT denotes all
T21462 7424-7433 NNS denotes stainings
T21463 7434-7436 TO denotes to
T21464 7437-7443 VB denotes reduce
T21465 7444-7455 JJ denotes nonspecific
T21466 7456-7464 NN denotes antibody
T21467 7465-7472 JJ denotes binding
T21468 7472-7473 . denotes .
T21469 7474-7476 TO denotes To
T21470 7477-7484 VB denotes isolate
T21471 7485-7490 JJ denotes naive
T21472 7491-7497 NN denotes murine
T21473 7498-7501 NNP denotes CD4
T21474 7502-7503 NNP denotes T
T21475 7504-7509 NNS denotes cells
T21476 7510-7514 IN denotes from
T21477 7515-7521 JJ denotes murine
T21478 7522-7526 NNP denotes DO11
T21479 7526-7529 CD denotes .10
T21480 7530-7532 CC denotes or
T21481 7533-7550 JJ denotes DO11.10xCD2-GATA3
T21482 7551-7552 NN denotes T
T21483 7553-7558 NNS denotes cells
T21484 7558-7559 , denotes ,
T21485 7560-7565 NNS denotes cells
T21486 7566-7570 VBD denotes were
T21487 7571-7578 VBN denotes stained
T21488 7579-7583 IN denotes with
T21489 7584-7598 JJ denotes anti-CD25-FITC
T21490 7598-7599 , denotes ,
T21491 7600-7613 JJ denotes anti-CD62L-PE
T21492 7613-7614 , denotes ,
T21493 7615-7618 CC denotes and
T21494 7619-7631 JJ denotes anti-CD4-APC
T21495 7632-7637 RB denotes prior
T21496 7638-7640 TO denotes to
T21497 7641-7648 VBG denotes sorting
T21498 7648-7649 . denotes .
T21499 7650-7654 NNP denotes Dead
T21500 7655-7660 NNS denotes cells
T21501 7661-7665 VBD denotes were
T21502 7666-7674 VBN denotes excluded
T21503 7675-7679 IN denotes with
T21504 7680-7681 CD denotes 4
T21505 7681-7682 NN denotes ′
T21506 7682-7683 '' denotes '
T21507 7683-7685 SYM denotes ,6
T21508 7685-7686 : denotes -
T21509 7686-7710 NN denotes Diamidino-2-phenylindole
T21510 7711-7712 -LRB- denotes (
T21511 7712-7716 NNP denotes DAPI
T21512 7716-7717 -RRB- denotes )
T21513 7717-7718 . denotes .
T21514 7719-7721 TO denotes To
T21515 7722-7729 VB denotes analyze
T21517 7737-7742 JJ denotes Foxp3
T21518 7743-7753 NN denotes expression
T21519 7754-7756 IN denotes in
T21520 7757-7766 JJ denotes inducible
T21521 7767-7771 NNP denotes Treg
T21522 7772-7780 NNS denotes cultures
T21523 7780-7781 , denotes ,
T21524 7782-7787 NNS denotes cells
T21525 7788-7792 VBD denotes were
T21526 7793-7800 VBN denotes stained
T21527 7801-7816 RB denotes intracellularly
T21528 7817-7821 IN denotes with
T21529 7822-7835 JJ denotes anti-Foxp3-PE
T21530 7836-7845 VBG denotes according
T21531 7846-7848 TO denotes to
T21532 7849-7861 NN denotes manufacturer
T21533 7861-7863 POS denotes 's
T21534 7864-7875 NN denotes instruction
T21535 7875-7876 , denotes ,
T21536 7877-7879 IN denotes in
T21537 7880-7891 NN denotes conjunction
T21538 7892-7896 IN denotes with
T21539 7897-7909 JJ denotes anti-CD4-APC
T21540 7910-7913 CC denotes and
T21541 7914-7923 NNP denotes LIVE/DEAD
T21542 7924-7931 JJ denotes fixable
T21543 7932-7936 JJ denotes dead
T21544 7937-7941 NN denotes cell
T21545 7942-7947 VB denotes stain
T21546 7948-7951 NN denotes kit
T21547 7952-7953 -LRB- denotes (
T21548 7953-7963 NNP denotes Invitrogen
T21549 7963-7964 -RRB- denotes )
T21550 7965-7967 TO denotes to
T21551 7968-7980 JJ denotes discriminate
T21552 7981-7985 JJ denotes live
T21553 7986-7991 NNS denotes cells
T21554 7991-7992 . denotes .
T21555 7993-7996 DT denotes All
T21556 7997-8007 JJ denotes monoclonal
T21557 8008-8018 NNS denotes antibodies
T21558 8019-8022 IN denotes for
T21559 8023-8029 JJ denotes murine
T21560 8030-8034 NN denotes cell
T21561 8035-8044 NNS denotes stainings
T21562 8045-8049 VBD denotes were
T21563 8050-8059 VBN denotes purchased
T21564 8060-8064 IN denotes from
T21565 8065-8076 NNP denotes eBioscience
T21566 8077-8079 CC denotes or
T21567 8080-8082 NNP denotes BD
T21568 8083-8094 NNPS denotes Biosciences
T21569 8094-8095 . denotes .
T21972 8097-8104 JJ denotes Western
T21973 8105-8113 VBG denotes blotting
T21974 8113-8114 . denotes .
T21975 8115-8118 IN denotes For
T21976 8119-8124 CD denotes FOXP3
T21977 8125-8133 NN denotes analysis
T21978 8134-8136 IN denotes on
T21979 8137-8140 DT denotes the
T21980 8141-8148 NN denotes protein
T21981 8149-8154 NN denotes level
T21982 8154-8155 , denotes ,
T21983 8156-8157 CD denotes 1
T21984 8158-8159 CD denotes ×
T21985 8160-8163 CD denotes 106
T21986 8164-8167 NNP denotes CD4
T21987 8167-8168 NNP denotes +
T21988 8168-8172 NNP denotes CD25
T21989 8174-8179 NNS denotes cells
T21990 8180-8184 VBD denotes were
T21991 8185-8190 VBN denotes lysed
T21992 8191-8194 CC denotes and
T21993 8195-8201 VBN denotes loaded
T21994 8202-8206 JJ denotes next
T21995 8207-8209 TO denotes to
T21996 8210-8211 DT denotes a
T21997 8212-8224 JJ denotes protein-mass
T21998 8225-8231 NN denotes ladder
T21999 8232-8233 -LRB- denotes (
T22000 8233-8242 NNP denotes Magicmark
T22001 8242-8243 , denotes ,
T22002 8244-8254 NNP denotes Invitrogen
T22003 8254-8255 -RRB- denotes )
T22004 8256-8258 IN denotes on
T22005 8259-8260 DT denotes a
T22006 8261-8267 NNP denotes NuPAGE
T22007 8268-8269 CD denotes 4
T22008 8270-8272 CD denotes 12
T22009 8272-8273 NN denotes %
T22010 8274-8282 JJ denotes bis-tris
T22011 8283-8286 NN denotes gel
T22012 8287-8288 -LRB- denotes (
T22013 8288-8298 NNP denotes Invitrogen
T22014 8298-8299 -RRB- denotes )
T22015 8299-8300 . denotes .
T22016 8301-8304 DT denotes The
T22017 8305-8313 NNS denotes proteins
T22018 8314-8318 VBD denotes were
T22019 8319-8333 VBN denotes electroblotted
T22020 8334-8338 IN denotes onto
T22021 8339-8340 DT denotes a
T22022 8341-8345 NNP denotes PVDF
T22023 8346-8354 NN denotes membrane
T22024 8355-8356 -LRB- denotes (
T22025 8356-8364 NNP denotes Amersham
T22026 8365-8369 NNP denotes Life
T22027 8370-8377 NNP denotes Science
T22028 8377-8378 -RRB- denotes )
T22029 8378-8379 . denotes .
T22030 8380-8390 JJ denotes Unspecific
T22031 8391-8398 JJ denotes binding
T22032 8399-8402 VBD denotes was
T22033 8403-8410 VBN denotes blocked
T22034 8411-8415 IN denotes with
T22035 8416-8419 NNP denotes BSA
T22036 8419-8420 , denotes ,
T22037 8421-8424 CC denotes and
T22038 8425-8428 DT denotes the
T22039 8429-8438 NNS denotes membranes
T22040 8439-8443 VBD denotes were
T22041 8444-8456 RB denotes subsequently
T22042 8457-8466 VBN denotes incubated
T22043 8467-8471 IN denotes with
T22044 8472-8474 DT denotes an
T22045 8475-8480 CD denotes 1:200
T22046 8481-8489 NN denotes dilution
T22047 8490-8492 IN denotes of
T22048 8493-8497 NN denotes goat
T22049 8498-8508 NN denotes anti-FOXP3
T22050 8509-8511 IN denotes in
T22051 8512-8520 VBG denotes blocking
T22052 8521-8527 NN denotes buffer
T22053 8528-8529 -LRB- denotes (
T22054 8529-8534 NNP denotes Abcam
T22055 8534-8535 -RRB- denotes )
T22056 8536-8545 RB denotes overnight
T22057 8546-8548 IN denotes at
T22058 8549-8550 CD denotes 4
T22059 8551-8552 CD denotes °
T22060 8552-8553 NNP denotes C
T22061 8553-8554 . denotes .
T22062 8555-8558 DT denotes The
T22063 8559-8564 NNS denotes blots
T22064 8565-8569 VBD denotes were
T22065 8570-8579 VBN denotes developed
T22066 8580-8585 VBG denotes using
T22067 8586-8588 DT denotes an
T22068 8589-8598 JJ denotes anti-goat
T22069 8599-8610 JJ denotes HRP-labeled
T22070 8611-8614 NNP denotes mAb
T22071 8615-8616 -LRB- denotes (
T22072 8616-8624 NNP denotes Amersham
T22073 8625-8636 NNPS denotes Biosciences
T22074 8636-8637 -RRB- denotes )
T22075 8638-8641 CC denotes and
T22076 8642-8652 VBN denotes visualized
T22077 8653-8657 IN denotes with
T22078 8658-8659 DT denotes a
T22079 8660-8663 NNP denotes LAS
T22080 8664-8668 CD denotes 1000
T22081 8669-8675 NN denotes camera
T22082 8676-8677 -LRB- denotes (
T22083 8677-8681 NNP denotes Fuji
T22084 8681-8682 -RRB- denotes )
T22085 8682-8683 . denotes .
T22086 8684-8693 NNS denotes Membranes
T22087 8694-8698 VBD denotes were
T22088 8699-8708 VBN denotes incubated
T22089 8709-8711 IN denotes in
T22090 8712-8721 VBG denotes stripping
T22091 8722-8728 NN denotes buffer
T22092 8729-8732 CC denotes and
T22093 8733-8743 JJ denotes re-blocked
T22094 8744-8747 IN denotes for
T22095 8748-8749 CD denotes 1
T22096 8750-8751 NN denotes h
T22097 8751-8752 . denotes .
T22098 8753-8756 DT denotes The
T22099 8757-8766 NNS denotes membranes
T22100 8767-8771 VBD denotes were
T22101 8772-8781 JJ denotes re-probed
T22102 8782-8787 VBG denotes using
T22103 8788-8798 JJ denotes anti-GATA3
T22104 8799-8800 -LRB- denotes (
T22105 8800-8803 CD denotes HG3
T22106 8804-8806 CD denotes 31
T22107 8806-8807 : denotes ;
T22108 8808-8813 NNP denotes Santa
T22109 8814-8818 NNP denotes Cruz
T22110 8819-8832 NNP denotes Biotechnology
T22111 8832-8833 -RRB- denotes )
T22112 8833-8834 , denotes ,
T22113 8835-8845 JJ denotes anti-T-bet
T22114 8846-8847 -LRB- denotes (
T22115 8847-8851 NNP denotes 4B10
T22116 8851-8852 , denotes ,
T22117 8853-8858 NNP denotes Santa
T22118 8859-8863 NNP denotes Cruz
T22119 8864-8877 NNP denotes Biotechnology
T22120 8877-8878 -RRB- denotes )
T22121 8878-8879 , denotes ,
T22122 8880-8890 JJ denotes anti-GAPDH
T22123 8891-8892 -LRB- denotes (
T22124 8892-8895 CD denotes 6C5
T22125 8895-8896 , denotes ,
T22126 8897-8903 NNP denotes Ambion
T22127 8903-8904 -RRB- denotes )
T22128 8904-8905 , denotes ,
T22129 8906-8924 JJ denotes anti-phospho-SMAD2
T22130 8925-8926 -LRB- denotes (
T22131 8926-8931 CD denotes 138D4
T22132 8931-8932 -RRB- denotes )
T22133 8932-8933 , denotes ,
T22134 8934-8952 JJ denotes anti-phospho-STAT6
T22135 8953-8954 -LRB- denotes (
T22136 8954-8957 NNP denotes 5A4
T22137 8957-8958 -RRB- denotes )
T22138 8958-8959 , denotes ,
T22139 8960-8963 CC denotes and
T22140 8964-8974 NNP denotes anti-STAT6
T22141 8975-8976 -LRB- denotes (
T22142 8976-8980 NNP denotes Cell
T22143 8981-8990 NNP denotes Signaling
T22144 8991-9001 NNP denotes Technology
T22145 9001-9002 -RRB- denotes )
T22146 9002-9003 , denotes ,
T22376 9005-9019 NNP denotes Administration
T22377 9020-9022 IN denotes of
T22378 9023-9032 NNS denotes cytokines
T22379 9033-9036 CC denotes and
T22380 9037-9047 NNS denotes antibodies
T22381 9048-9050 IN denotes in
T22382 9051-9055 NN denotes vivo
T22383 9055-9056 . denotes .
T22384 9057-9060 NNP denotes Age
T22385 9060-9061 : denotes -
T22386 9062-9065 CC denotes and
T22387 9066-9080 JJ denotes gender-matched
T22388 9081-9087 JJ denotes normal
T22389 9088-9090 NNP denotes B6
T22390 9091-9095 NNS denotes mice
T22391 9096-9104 VBD denotes received
T22392 9105-9110 DT denotes every
T22393 9111-9116 JJ denotes other
T22394 9117-9120 NN denotes day
T22395 9121-9136 JJ denotes intraperitoneal
T22396 9137-9138 -LRB- denotes (
T22397 9138-9140 NN denotes ip
T22398 9140-9141 -RRB- denotes )
T22399 9142-9152 NNS denotes injections
T22400 9153-9155 IN denotes of
T22401 9156-9159 NNP denotes PBS
T22402 9159-9160 , denotes ,
T22403 9161-9164 CD denotes 1.5
T22404 9165-9167 NN denotes μg
T22405 9168-9174 NN denotes rmIL-4
T22406 9174-9175 , denotes ,
T22407 9176-9178 CD denotes 50
T22408 9179-9181 NN denotes μg
T22409 9182-9191 JJ denotes anti-IL-4
T22410 9192-9195 NNP denotes mAb
T22411 9196-9197 -LRB- denotes (
T22412 9197-9202 NNP denotes 11B11
T22413 9203-9205 CC denotes or
T22414 9206-9212 NNP denotes MAB404
T22415 9212-9213 -RRB- denotes )
T22416 9213-9214 , denotes ,
T22417 9215-9217 CC denotes or
T22418 9218-9219 DT denotes a
T22419 9220-9227 NN denotes mixture
T22420 9228-9230 IN denotes of
T22421 9231-9234 CD denotes 1.5
T22422 9235-9237 NN denotes μg
T22423 9238-9244 NN denotes rmIL-4
T22424 9245-9249 CC denotes plus
T22425 9250-9252 CD denotes 50
T22426 9253-9255 NN denotes μg
T22427 9256-9265 JJ denotes anti-IL-4
T22428 9266-9269 NNP denotes mAb
T22429 9270-9271 -LRB- denotes (
T22430 9271-9276 NNP denotes 11B11
T22431 9277-9279 CC denotes or
T22432 9280-9286 NNP denotes MAB404
T22433 9286-9287 -RRB- denotes )
T22434 9288-9291 IN denotes for
T22435 9292-9293 CD denotes 7
T22436 9294-9296 NN denotes d.
T22437 9297-9307 RB denotes Thereafter
T22438 9307-9308 , denotes ,
T22439 9309-9315 JJ denotes spleen
T22440 9316-9319 CC denotes and
T22441 9320-9325 JJ denotes lymph
T22442 9326-9330 NN denotes node
T22443 9331-9336 NNS denotes cells
T22444 9337-9341 VBD denotes were
T22445 9342-9350 VBN denotes analyzed
T22446 9351-9353 IN denotes by
T22447 9354-9358 NN denotes flow
T22448 9359-9368 NN denotes cytometry
T22449 9369-9372 IN denotes for
T22450 9373-9376 NNP denotes CD3
T22451 9376-9377 , denotes ,
T22452 9378-9381 NNP denotes CD4
T22453 9381-9382 , denotes ,
T22454 9383-9386 CC denotes and
T22455 9387-9391 NNP denotes CD25
T22456 9392-9402 NN denotes expression
T22457 9402-9403 . denotes .
T22458 9404-9407 DT denotes The
T22459 9408-9418 JJ denotes anti-mouse
T22460 9419-9423 NN denotes IL-4
T22461 9424-9427 NNP denotes mAb
T22462 9428-9434 NNP denotes MAB404
T22463 9435-9438 VBD denotes was
T22464 9439-9447 VBN denotes obtained
T22465 9448-9452 IN denotes from
T22466 9453-9456 NNP denotes R&D
T22467 9457-9464 NNPS denotes Systems
T22468 9464-9465 , denotes ,
T22469 9466-9469 DT denotes the
T22470 9470-9476 JJ denotes second
T22471 9477-9487 JJ denotes anti-mouse
T22472 9488-9492 NN denotes IL-4
T22473 9493-9496 NNP denotes mAb
T22474 9497-9502 NNP denotes 11B11
T22475 9503-9506 VBD denotes was
T22476 9507-9516 VBN denotes purchased
T22477 9517-9521 IN denotes from
T22478 9522-9533 NNP denotes eBioscience
T22479 9533-9534 . denotes .
T22754 9536-9540 NN denotes ChIP
T22755 9540-9541 . denotes .
T22756 9542-9546 NN denotes ChIP
T22757 9547-9555 NN denotes analysis
T22758 9556-9559 VBD denotes was
T22759 9560-9569 VBN denotes performed
T22760 9570-9579 VBG denotes according
T22761 9580-9582 TO denotes to
T22762 9583-9586 DT denotes the
T22763 9587-9599 NN denotes manufacturer
T22764 9599-9601 POS denotes 's
T22765 9602-9610 NN denotes protocol
T22766 9611-9612 -LRB- denotes (
T22767 9612-9619 NNP denotes Upstate
T22768 9620-9633 NNP denotes Biotechnology
T22769 9633-9634 -RRB- denotes )
T22770 9635-9639 IN denotes with
T22771 9640-9643 DT denotes the
T22772 9644-9653 VBG denotes following
T22773 9654-9667 NNS denotes modifications
T22774 9667-9668 . denotes .
T22775 9669-9674 NNP denotes iTreg
T22776 9675-9678 CC denotes and
T22777 9679-9682 NNP denotes Th2
T22778 9683-9688 NNS denotes cells
T22779 9689-9693 VBD denotes were
T22780 9694-9699 VBN denotes fixed
T22781 9700-9704 IN denotes with
T22782 9705-9706 CD denotes 1
T22783 9706-9707 NN denotes %
T22784 9708-9720 NN denotes formaldehyde
T22785 9721-9724 IN denotes for
T22786 9725-9727 CD denotes 10
T22787 9728-9731 NN denotes min
T22788 9732-9734 IN denotes at
T22789 9735-9739 NN denotes room
T22790 9740-9751 NN denotes temperature
T22791 9751-9752 . denotes .
T22792 9753-9756 DT denotes The
T22793 9757-9766 NN denotes chromatin
T22794 9767-9770 VBD denotes was
T22795 9771-9778 VBN denotes sheared
T22796 9779-9781 TO denotes to
T22797 9782-9790 CD denotes 200-1000
T22798 9791-9793 NN denotes bp
T22799 9794-9796 IN denotes of
T22800 9797-9803 NN denotes length
T22801 9804-9806 IN denotes by
T22802 9807-9817 NN denotes sonication
T22803 9818-9822 IN denotes with
T22804 9823-9827 CD denotes five
T22805 9828-9834 NNS denotes pulses
T22806 9835-9837 IN denotes of
T22807 9838-9840 CD denotes 10
T22808 9841-9842 PRP denotes s
T22809 9843-9845 IN denotes at
T22810 9846-9848 CD denotes 30
T22811 9848-9849 NN denotes %
T22812 9850-9855 NN denotes power
T22813 9856-9857 -LRB- denotes (
T22814 9857-9865 NNP denotes Bandelin
T22815 9865-9866 -RRB- denotes )
T22816 9866-9867 . denotes .
T22817 9868-9871 DT denotes The
T22818 9872-9881 NN denotes chromatin
T22819 9882-9885 VBD denotes was
T22820 9886-9897 JJ denotes pre-cleared
T22821 9898-9901 IN denotes for
T22822 9902-9903 CD denotes 2
T22823 9904-9905 NN denotes h
T22824 9906-9910 IN denotes with
T22825 9911-9917 JJ denotes normal
T22826 9918-9923 NN denotes mouse
T22827 9924-9927 NNP denotes IgG
T22828 9928-9933 NNS denotes beads
T22829 9934-9937 CC denotes and
T22830 9938-9942 RB denotes then
T22831 9943-9952 VBN denotes incubated
T22832 9953-9957 IN denotes with
T22833 9958-9976 JJ denotes anti-GATA3-agarose
T22834 9977-9982 NNS denotes beads
T22835 9983-9984 -LRB- denotes (
T22836 9984-9987 CD denotes HG3
T22837 9988-9990 CD denotes 31
T22838 9990-9991 : denotes ;
T22839 9992-9997 NNP denotes Santa
T22840 9998-10002 NNP denotes Cruz
T22841 10003-10016 NNP denotes Biotechnology
T22842 10016-10017 -RRB- denotes )
T22843 10018-10021 IN denotes for
T22844 10022-10023 CD denotes 2
T22845 10024-10026 NN denotes h.
T22846 10027-10034 VBG denotes Washing
T22847 10035-10038 CC denotes and
T22848 10039-10046 NN denotes elution
T22849 10047-10054 NNS denotes buffers
T22850 10055-10059 VBD denotes were
T22851 10060-10064 VBN denotes used
T22852 10065-10074 VBG denotes according
T22853 10075-10077 TO denotes to
T22854 10078-10081 DT denotes the
T22855 10082-10090 NN denotes protocol
T22856 10091-10093 IN denotes of
T22857 10094-10101 NNP denotes Upstate
T22858 10102-10115 NNP denotes Biotechnology
T22859 10115-10116 . denotes .
T22860 10117-10127 NNS denotes Crosslinks
T22861 10128-10132 VBD denotes were
T22862 10133-10141 VBN denotes reversed
T22863 10142-10144 IN denotes by
T22864 10145-10155 NN denotes incubation
T22865 10156-10158 IN denotes at
T22866 10159-10161 CD denotes 65
T22867 10162-10163 CD denotes °
T22868 10163-10164 NNP denotes C
T22869 10165-10168 IN denotes for
T22870 10169-10170 CD denotes 4
T22871 10171-10172 NN denotes h
T22872 10173-10175 IN denotes in
T22873 10176-10179 DT denotes the
T22874 10180-10188 NN denotes presence
T22875 10189-10191 IN denotes of
T22876 10192-10195 CD denotes 0.2
T22877 10196-10197 NNP denotes M
T22878 10198-10202 NNP denotes NaCl
T22879 10202-10203 , denotes ,
T22880 10204-10207 CC denotes and
T22881 10208-10211 DT denotes the
T22882 10212-10215 NN denotes DNA
T22883 10216-10219 VBD denotes was
T22884 10220-10228 VBN denotes purified
T22885 10229-10231 IN denotes by
T22886 10232-10249 NN denotes phenol/chloroform
T22887 10250-10260 NN denotes extraction
T22888 10260-10261 . denotes .
T22889 10262-10265 DT denotes The
T22890 10266-10272 NN denotes amount
T22891 10273-10275 IN denotes of
T22892 10276-10279 NNP denotes DNA
T22893 10280-10283 VBD denotes was
T22894 10284-10294 VBN denotes determined
T22895 10295-10297 IN denotes by
T22896 10298-10310 JJ denotes conventional
T22897 10311-10314 NNP denotes PCR
T22898 10314-10315 . denotes .
T22899 10316-10319 DT denotes The
T22900 10320-10323 NNP denotes PCR
T22901 10324-10333 VBD denotes addressed
T22902 10334-10337 IN denotes for
T22903 10338-10341 DT denotes the
T22904 10342-10347 CD denotes FOXP3
T22905 10348-10356 NN denotes promoter
T22906 10357-10363 NN denotes region
T22907 10364-10365 NN denotes −
T22908 10365-10368 CD denotes 246
T22909 10369-10371 TO denotes to
T22910 10372-10373 CD denotes −
T22911 10373-10376 CD denotes 511
T22912 10377-10380 CC denotes and
T22913 10381-10384 VBD denotes was
T22914 10385-10394 VBN denotes performed
T22915 10395-10400 VBG denotes using
T22916 10401-10404 DT denotes the
T22917 10405-10414 VBG denotes following
T22918 10415-10422 NNS denotes primers
T22919 10422-10423 : denotes :
T22920 10424-10425 CD denotes 5
T22921 10425-10426 SYM denotes ′
T22922 10426-10427 : denotes -
T22923 10427-10442 NN denotes gtgccctttacgagt
T22924 10443-10451 JJ denotes catctg-3
T22925 10451-10452 NN denotes ′
T22926 10453-10456 CC denotes and
T22927 10457-10458 CD denotes 5
T22928 10458-10459 SYM denotes ′
T22929 10459-10460 : denotes -
T22930 10460-10483 JJ denotes gtgccctttacgagtcatctg-3
T22931 10483-10484 NN denotes ′
T22932 10484-10485 . denotes .
T22933 10486-10489 DT denotes The
T22934 10490-10493 NNP denotes PCR
T22935 10494-10502 NNS denotes products
T22936 10503-10507 VBD denotes were
T22937 10508-10518 VBN denotes visualized
T22938 10519-10524 VBG denotes using
T22939 10525-10527 DT denotes an
T22940 10528-10536 NN denotes ethidium
T22941 10537-10544 NN denotes bromide
T22942 10545-10548 NN denotes gel
T22943 10548-10549 . denotes .
T23192 10551-10560 JJ denotes Pull-down
T23193 10561-10566 NN denotes assay
T23194 10566-10567 . denotes .
T23195 10568-10571 CD denotes CD4
T23196 10571-10572 CD denotes +
T23197 10573-10574 NNP denotes T
T23198 10575-10580 NNS denotes cells
T23199 10581-10585 VBD denotes were
T23200 10586-10596 VBN denotes stimulated
T23201 10597-10601 IN denotes with
T23202 10602-10605 NNP denotes PMA
T23203 10606-10609 CC denotes and
T23204 10610-10619 NN denotes ionomycin
T23205 10620-10623 IN denotes for
T23206 10624-10625 CD denotes 2
T23207 10626-10627 NN denotes h
T23208 10628-10630 IN denotes at
T23209 10631-10633 CD denotes 37
T23210 10633-10634 CD denotes °
T23211 10634-10635 NNP denotes C
T23212 10635-10636 . denotes .
T23213 10637-10640 DT denotes The
T23214 10641-10646 NNS denotes cells
T23215 10647-10651 VBD denotes were
T23216 10652-10660 VBN denotes pelleted
T23217 10660-10661 , denotes ,
T23218 10662-10673 VBN denotes resuspended
T23219 10674-10676 IN denotes in
T23220 10677-10683 NN denotes buffer
T23221 10684-10685 NNP denotes C
T23222 10686-10687 -LRB- denotes (
T23223 10687-10689 CD denotes 20
T23224 10690-10692 NNP denotes mM
T23225 10693-10698 NNP denotes HEPES
T23226 10699-10700 NNP denotes [
T23227 10700-10702 NNP denotes pH
T23228 10703-10706 CD denotes 7.9
T23229 10706-10707 NNP denotes ]
T23230 10707-10708 , denotes ,
T23231 10709-10712 CD denotes 420
T23232 10713-10715 NNP denotes mM
T23233 10716-10720 NNP denotes NaCl
T23234 10720-10721 , denotes ,
T23235 10722-10725 CD denotes 1.5
T23236 10726-10728 NNP denotes mM
T23237 10729-10734 NNP denotes MgCl2
T23238 10734-10735 , denotes ,
T23239 10736-10739 CD denotes 0.2
T23240 10740-10742 NNP denotes mM
T23241 10743-10747 NNP denotes EDTA
T23242 10747-10748 , denotes ,
T23243 10749-10750 CD denotes 1
T23244 10751-10753 NNP denotes mM
T23245 10754-10757 NNP denotes DTT
T23246 10757-10758 , denotes ,
T23247 10759-10767 NN denotes protease
T23248 10768-10778 NNS denotes inhibitors
T23249 10779-10780 NNP denotes [
T23250 10780-10785 NNP denotes Sigma
T23251 10785-10786 NNP denotes ]
T23252 10786-10787 . denotes .
T23253 10788-10791 CC denotes and
T23254 10792-10795 CD denotes 0.1
T23255 10795-10796 NN denotes %
T23256 10797-10802 NN denotes NP-40
T23257 10802-10803 -RRB- denotes )
T23258 10804-10807 CC denotes and
T23259 10808-10813 VBN denotes lysed
T23260 10814-10816 IN denotes on
T23261 10817-10820 NN denotes ice
T23262 10821-10824 IN denotes for
T23263 10825-10827 CD denotes 15
T23264 10828-10831 NN denotes min
T23265 10831-10832 . denotes .
T23266 10833-10842 NNP denotes Insoluble
T23267 10843-10851 NN denotes material
T23268 10852-10855 VBD denotes was
T23269 10856-10863 VBN denotes removed
T23270 10864-10866 IN denotes by
T23271 10867-10881 NN denotes centrifugation
T23272 10881-10882 . denotes .
T23273 10883-10886 DT denotes The
T23274 10887-10898 NN denotes supernatant
T23275 10899-10902 VBD denotes was
T23276 10903-10910 VBN denotes diluted
T23277 10911-10914 CD denotes 1:3
T23278 10915-10919 IN denotes with
T23279 10920-10926 NN denotes buffer
T23280 10927-10928 NNP denotes D
T23281 10929-10930 -LRB- denotes (
T23282 10930-10932 IN denotes as
T23283 10933-10939 NN denotes buffer
T23284 10940-10941 NNP denotes C
T23285 10941-10942 , denotes ,
T23286 10943-10946 CC denotes but
T23287 10947-10954 IN denotes without
T23288 10955-10959 NNP denotes NaCl
T23289 10959-10960 -RRB- denotes )
T23290 10960-10961 . denotes .
T23291 10962-10965 DT denotes The
T23292 10966-10973 NNS denotes lysates
T23293 10974-10978 VBD denotes were
T23294 10979-10988 VBN denotes incubated
T23295 10989-10993 IN denotes with
T23296 10994-10996 CD denotes 10
T23297 10997-10999 NN denotes μg
T23298 11000-11002 IN denotes of
T23299 11003-11007 NN denotes poly
T23300 11007-11008 -LRB- denotes (
T23301 11008-11013 JJ denotes dI-dC
T23302 11013-11014 -RRB- denotes )
T23303 11015-11016 -LRB- denotes (
T23304 11016-11021 NNP denotes Sigma
T23305 11021-11022 -RRB- denotes )
T23306 11023-11026 CC denotes and
T23307 11027-11029 CD denotes 70
T23308 11030-11032 NN denotes μl
T23309 11033-11035 IN denotes of
T23310 11036-11056 JJ denotes streptavidin-agarose
T23311 11057-11058 -LRB- denotes (
T23312 11058-11066 NNP denotes Amersham
T23313 11067-11078 NNPS denotes Biosciences
T23314 11078-11079 -RRB- denotes )
T23315 11080-11088 VBG denotes carrying
T23316 11089-11101 JJ denotes biotinylated
T23317 11102-11118 NNS denotes oligonucleotides
T23318 11118-11119 , denotes ,
T23319 11120-11123 IN denotes for
T23320 11124-11125 CD denotes 3
T23321 11126-11127 NN denotes h
T23322 11128-11130 IN denotes at
T23323 11131-11132 CD denotes 4
T23324 11133-11134 CD denotes °
T23325 11134-11135 NNP denotes C
T23326 11135-11136 . denotes .
T23327 11137-11140 DT denotes The
T23328 11141-11146 NNS denotes beads
T23329 11147-11151 VBD denotes were
T23330 11152-11158 VBN denotes washed
T23331 11159-11164 RB denotes twice
T23332 11165-11169 IN denotes with
T23333 11170-11176 NN denotes buffer
T23334 11177-11178 NNP denotes C
T23335 11178-11180 NNP denotes :D
T23336 11181-11182 -LRB- denotes (
T23337 11182-11185 CD denotes 1:3
T23338 11185-11186 -RRB- denotes )
T23339 11187-11190 CC denotes and
T23340 11191-11202 VBD denotes resuspended
T23341 11203-11205 IN denotes in
T23342 11206-11220 JJ denotes DTT-containing
T23343 11221-11228 VBG denotes loading
T23344 11229-11235 NN denotes buffer
T23345 11236-11237 -LRB- denotes (
T23346 11237-11243 NNP denotes NuPAGE
T23347 11243-11244 : denotes ;
T23348 11245-11255 NNP denotes Invitrogen
T23349 11255-11256 -RRB- denotes )
T23350 11256-11257 , denotes ,
T23351 11258-11264 VBN denotes heated
T23352 11265-11267 TO denotes to
T23353 11268-11270 CD denotes 70
T23354 11271-11272 CD denotes °
T23355 11272-11273 NNP denotes C
T23356 11274-11277 IN denotes for
T23357 11278-11280 CD denotes 10
T23358 11281-11284 NN denotes min
T23359 11284-11285 , denotes ,
T23360 11286-11289 CC denotes and
T23361 11290-11293 DT denotes the
T23362 11294-11301 NNS denotes eluants
T23363 11302-11304 IN denotes on
T23364 11305-11306 DT denotes a
T23365 11307-11313 NNP denotes NuPAGE
T23366 11314-11315 CD denotes 4
T23367 11316-11318 CD denotes 12
T23368 11318-11319 NN denotes %
T23369 11320-11328 JJ denotes bis-tris
T23370 11329-11332 NN denotes gel
T23371 11333-11334 -LRB- denotes (
T23372 11334-11344 NNP denotes Invitrogen
T23373 11344-11345 -RRB- denotes )
T23374 11345-11346 . denotes .
T23375 11347-11350 DT denotes The
T23376 11351-11359 NNS denotes proteins
T23377 11360-11364 VBD denotes were
T23378 11365-11379 VBN denotes electroblotted
T23379 11380-11384 IN denotes onto
T23380 11385-11386 DT denotes a
T23381 11387-11391 NNP denotes PVDF
T23382 11392-11400 NN denotes membrane
T23383 11401-11402 -LRB- denotes (
T23384 11402-11410 NNP denotes Amersham
T23385 11411-11422 NNPS denotes Biosciences
T23386 11422-11423 -RRB- denotes )
T23387 11424-11427 CC denotes and
T23388 11428-11436 VBD denotes detected
T23389 11437-11442 VBG denotes using
T23390 11443-11445 DT denotes an
T23391 11446-11456 JJ denotes anti-GATA3
T23392 11457-11460 NNP denotes mAb
T23393 11461-11462 -LRB- denotes (
T23394 11462-11467 NNP denotes Santa
T23395 11468-11472 NNP denotes Cruz
T23396 11473-11486 NNP denotes Biotechnology
T23397 11486-11487 -RRB- denotes )
T23398 11487-11488 . denotes .
T23399 11489-11500 VBN denotes Accumulated
T23400 11501-11508 NNS denotes signals
T23401 11509-11513 VBD denotes were
T23402 11514-11522 VBN denotes analyzed
T23403 11523-11528 VBG denotes using
T23404 11529-11533 NNP denotes AIDA
T23405 11534-11542 NN denotes software
T23406 11543-11544 -LRB- denotes (
T23407 11544-11551 JJS denotes Raytest
T23408 11551-11552 -RRB- denotes )
T23409 11552-11553 . denotes .
R13523 T17891 T17891 ROOT Mice,Mice
R13598 T17966 T17965 prep for,background
R13599 T17967 T17968 det a,minimum
R13600 T17968 T17966 pobj minimum,for
R13601 T17969 T17968 prep of,minimum
R13602 T17970 T17971 nummod eight,generations
R13603 T17971 T17969 pobj generations,of
R13604 T17972 T17961 cc and,backcrossed
R13605 T17973 T17961 conj used,backcrossed
R13606 T17974 T17973 prep at,used
R13607 T17975 T17976 det an,age
R13608 T17976 T17974 pobj age,at
R13609 T17977 T17976 prep of,age
R13610 T17978 T17977 pobj 8,of
R13611 T17979 T17980 nummod 12,wk
R13612 T17980 T17978 appos wk,8
R13613 T17981 T17961 punct .,backcrossed
R13614 T17982 T17984 nsubjpass Mice,housed
R13615 T17983 T17984 auxpass were,housed
R13616 T17984 T17984 ROOT housed,housed
R13617 T17985 T17984 prep under,housed
R13618 T17986 T17988 amod specific,conditions
R13619 T17987 T17988 amod pathogen-free,conditions
R13620 T17988 T17985 pobj conditions,under
R13621 T17989 T17988 cc and,conditions
R13622 T17990 T17992 det all,studies
R13623 T17991 T17992 compound animal,studies
R13624 T17992 T17994 nsubjpass studies,performed
R13625 T17993 T17994 auxpass were,performed
R13626 T17994 T17984 conj performed,housed
R13627 T17995 T17994 prep according,performed
R13628 T17996 T17995 prep to,according
R13629 T17997 T18000 amod institutional,guidelines
R13630 T17998 T17997 cc and,institutional
R13631 T17999 T17997 conj state,institutional
R13632 T18000 T17996 pobj guidelines,to
R13633 T18001 T17994 punct .,performed
R13728 T18166 T18166 ROOT Isolation,Isolation
R13729 T18167 T18166 prep of,Isolation
R13730 T18168 T18171 nummod CD4,cells
R13731 T18169 T18171 nummod +,cells
R13732 T18170 T18171 compound T,cells
R13733 T18171 T18167 pobj cells,of
R13734 T18172 T18166 punct .,Isolation
R13735 T18173 T18178 nsubjpass CD4,isolated
R13736 T18174 T18176 nummod +,cells
R13737 T18175 T18176 compound T,cells
R13738 T18176 T18177 nsubj cells,were
R13739 T18177 T18178 auxpass were,isolated
R13740 T18178 T18178 ROOT isolated,isolated
R13741 T18179 T18178 prep from,isolated
R13742 T18180 T18179 pobj blood,from
R13743 T18181 T18180 prep of,blood
R13744 T18182 T18184 amod healthy,volunteers
R13745 T18183 T18184 amod human,volunteers
R13746 T18184 T18181 pobj volunteers,of
R13747 T18185 T18184 acl using,volunteers
R13748 T18186 T18189 det the,beads
R13749 T18187 T18189 amod anti-CD4,beads
R13750 T18188 T18189 amod magnetic,beads
R13751 T18189 T18185 dobj beads,using
R13752 T18190 T18189 punct (,beads
R13753 T18191 T18189 appos Dynal,beads
R13754 T18192 T18191 punct ",",Dynal
R13755 T18193 T18191 conj Hamburg,Dynal
R13756 T18194 T18193 punct ",",Hamburg
R13757 T18195 T18193 appos Germany,Hamburg
R13758 T18196 T18193 punct ),Hamburg
R13759 T18197 T18199 mark as,described
R13760 T18198 T18199 advmod previously,described
R13761 T18199 T18178 advcl described,isolated
R13762 T18200 T18202 nmod [,]
R13763 T18201 T18202 nummod 60,]
R13764 T18202 T18199 dobj ],described
R13765 T18203 T18178 punct .,isolated
R13766 T18204 T18205 det The,purity
R13767 T18205 T18213 nsubjpass purity,tested
R13768 T18206 T18205 prep of,purity
R13769 T18207 T18210 nummod CD4,cells
R13770 T18208 T18210 nummod +,cells
R13771 T18209 T18210 compound T,cells
R13772 T18210 T18206 pobj cells,of
R13773 T18211 T18213 auxpass was,tested
R13774 T18212 T18213 advmod initially,tested
R13775 T18213 T18213 ROOT tested,tested
R13776 T18214 T18213 agent by,tested
R13777 T18215 T18214 pobj FACS,by
R13778 T18216 T18213 cc and,tested
R13779 T18217 T18218 auxpass was,≥
R13780 T18218 T18213 conj ≥,tested
R13781 T18219 T18220 nummod 95,%
R13782 T18220 T18218 npadvmod %,≥
R13783 T18221 T18213 punct .,tested
R13784 T18222 T18228 nsubjpass Monoclonality,confirmed
R13785 T18223 T18222 prep of,Monoclonality
R13786 T18224 T18226 compound T,clones
R13787 T18225 T18226 compound cell,clones
R13788 T18226 T18223 pobj clones,of
R13789 T18227 T18228 auxpass was,confirmed
R13790 T18228 T18228 ROOT confirmed,confirmed
R13791 T18229 T18228 agent by,confirmed
R13792 T18230 T18231 amod TCR-chain,mapping
R13793 T18231 T18229 pobj mapping,by
R13794 T18232 T18228 cc and,confirmed
R13795 T18233 T18234 auxpass was,identified
R13796 T18234 T18228 conj identified,confirmed
R13797 T18235 T18236 aux to,be
R13798 T18236 T18234 xcomp be,identified
R13799 T18237 T18238 amod Vbeta8,positive
R13800 T18238 T18236 acomp positive,be
R13801 T18239 T18228 punct .,confirmed
R13802 T18240 T18241 det The,clones
R13803 T18241 T18243 nsubjpass clones,characterized
R13804 T18242 T18243 auxpass were,characterized
R13805 T18243 T18243 ROOT characterized,characterized
R13806 T18244 T18243 agent by,characterized
R13807 T18245 T18247 amod high,secretion
R13808 T18246 T18247 compound IL-4,secretion
R13809 T18247 T18244 pobj secretion,by
R13810 T18248 T18243 punct .,characterized
R13825 T18362 T18364 amod Quantitative,PCR
R13826 T18363 T18364 amod real-time,PCR
R13827 T18364 T18364 ROOT PCR,PCR
R13828 T18365 T18364 punct .,PCR
R13829 T18366 T18368 det The,primers
R13830 T18367 T18368 compound PCR,primers
R13831 T18368 T18372 nsubjpass primers,designed
R13832 T18369 T18368 cc and,primers
R13833 T18370 T18368 conj probes,primers
R13834 T18371 T18372 auxpass were,designed
R13835 T18372 T18372 ROOT designed,designed
R13836 T18373 T18372 prep based,designed
R13837 T18374 T18373 prep on,based
R13838 T18375 T18376 det the,sequences
R13839 T18376 T18374 pobj sequences,on
R13840 T18377 T18376 acl reported,sequences
R13841 T18378 T18377 prep in,reported
R13842 T18379 T18378 pobj GenBank,in
R13843 T18380 T18377 prep with,reported
R13844 T18381 T18385 det the,version
R13845 T18382 T18383 compound Primer,Express
R13846 T18383 T18385 compound Express,version
R13847 T18384 T18385 compound software,version
R13848 T18385 T18380 pobj version,with
R13849 T18386 T18385 nummod 1.2,version
R13850 T18387 T18389 punct (,Biosystems
R13851 T18388 T18389 compound Applied,Biosystems
R13852 T18389 T18385 appos Biosystems,version
R13853 T18390 T18377 punct ),reported
R13854 T18391 T18392 mark as,follows
R13855 T18392 T18372 advcl follows,designed
R13856 T18393 T18372 punct :,designed
R13857 T18394 T18396 nummod FOXP3,primer
R13858 T18395 T18396 amod forward,primer
R13859 T18396 T18396 ROOT primer,primer
R13860 T18397 T18438 meta 5,primer
R13861 T18398 T18400 punct ′,GAA
R13862 T18399 T18400 punct -,GAA
R13863 T18400 T18438 ccomp GAA,primer
R13864 T18401 T18402 compound ACA,GCA
R13865 T18402 T18426 npadvmod GCA,primer
R13866 T18403 T18426 nmod CAT,primer
R13867 T18404 T18408 nmod TCC,′
R13868 T18405 T18408 nmod CAG,′
R13869 T18406 T18408 nmod AGT,′
R13870 T18407 T18408 amod TC-3,′
R13871 T18408 T18403 appos ′,CAT
R13872 T18409 T18403 punct ;,CAT
R13873 T18410 T18412 nummod FOXP3,primer
R13874 T18411 T18412 compound reverse,primer
R13875 T18412 T18403 appos primer,CAT
R13876 T18413 T18426 nummod 5,primer
R13877 T18414 T18426 punct ′,primer
R13878 T18415 T18426 punct -,primer
R13879 T18416 T18417 compound ATG,GCC
R13880 T18417 T18426 npadvmod GCC,primer
R13881 T18418 T18422 nmod CAG,′
R13882 T18419 T18422 nmod CGG,′
R13883 T18420 T18422 nmod ATG,′
R13884 T18421 T18422 amod AG-3,′
R13885 T18422 T18417 appos ′,GCC
R13886 T18423 T18417 punct ;,GCC
R13887 T18424 T18417 conj EF-1α,GCC
R13888 T18425 T18417 advmod forward,GCC
R13889 T18426 T18400 amod primer,GAA
R13890 T18427 T18426 cc and,primer
R13891 T18428 T18426 conj reverse,primer
R13892 T18429 T18428 dobj primer,reverse
R13893 T18430 T18431 mark as,described
R13894 T18431 T18438 advcl described,primer
R13895 T18432 T18434 nmod [,]
R13896 T18433 T18434 nummod 61,]
R13897 T18434 T18431 dobj ],described
R13898 T18435 T18438 punct ;,primer
R13899 T18436 T18438 nsubj GATA3,primer
R13900 T18437 T18438 advmod forward,primer
R13901 T18438 T18438 ROOT primer,primer
R13902 T18439 T18462 nummod 5,′
R13903 T18440 T18444 punct ′,TCT
R13904 T18441 T18444 punct -,TCT
R13905 T18442 T18444 compound GCG,TCT
R13906 T18443 T18444 compound GGC,TCT
R13907 T18444 T18462 nmod TCT,′
R13908 T18445 T18446 compound ATC,ACA
R13909 T18446 T18447 compound ACA,AAA
R13910 T18447 T18462 dep AAA,′
R13911 T18448 T18449 nummod TGA-3,′
R13912 T18449 T18462 nmod ′,′
R13913 T18450 T18449 cc and,′
R13914 T18451 T18449 conj rwd,′
R13915 T18452 T18451 nummod 5,rwd
R13916 T18453 T18462 punct ′,′
R13917 T18454 T18455 punct -,GCT
R13918 T18455 T18462 compound GCT,′
R13919 T18456 T18462 compound CTC,′
R13920 T18457 T18462 compound CTG,′
R13921 T18458 T18462 nmod GCT,′
R13922 T18459 T18460 compound GCA,GAC
R13923 T18460 T18462 nmod GAC,′
R13924 T18461 T18462 amod AGC-3,′
R13925 T18462 T18438 dep ′,primer
R13926 T18463 T18438 punct .,primer
R13927 T18464 T18466 det The,cDNAs
R13928 T18465 T18466 amod prepared,cDNAs
R13929 T18466 T18468 nsubjpass cDNAs,amplified
R13930 T18467 T18468 auxpass were,amplified
R13931 T18468 T18468 ROOT amplified,amplified
R13932 T18469 T18468 advcl using,amplified
R13933 T18470 T18471 compound SYBR-PCR,mastermix
R13934 T18471 T18469 dobj mastermix,using
R13935 T18472 T18471 punct (,mastermix
R13936 T18473 T18471 appos Biorad,mastermix
R13937 T18474 T18471 punct ),mastermix
R13938 T18475 T18468 prep according,amplified
R13939 T18476 T18475 prep to,according
R13940 T18477 T18478 det the,recommendations
R13941 T18478 T18476 pobj recommendations,to
R13942 T18479 T18478 prep of,recommendations
R13943 T18480 T18481 det the,manufacturer
R13944 T18481 T18479 pobj manufacturer,of
R13945 T18482 T18478 prep in,recommendations
R13946 T18483 T18489 det an,System
R13947 T18484 T18489 compound ABI,System
R13948 T18485 T18489 compound PRISM,System
R13949 T18486 T18489 nummod 7000,System
R13950 T18487 T18489 compound Sequence,System
R13951 T18488 T18489 compound Detection,System
R13952 T18489 T18482 pobj System,in
R13953 T18490 T18489 punct (,System
R13954 T18491 T18492 compound Applied,Biosystems
R13955 T18492 T18489 appos Biosystems,System
R13956 T18493 T18489 punct ),System
R13957 T18494 T18468 punct .,amplified
R13958 T18495 T18496 compound Quantitative,PCR
R13959 T18496 T18501 nsubjpass PCR,performed
R13960 T18497 T18496 prep of,PCR
R13961 T18498 T18499 compound murine,samples
R13962 T18499 T18497 pobj samples,of
R13963 T18500 T18501 auxpass was,performed
R13964 T18501 T18501 ROOT performed,performed
R13965 T18502 T18501 prep with,performed
R13966 T18503 T18508 amod Brilliant,mix
R13967 T18504 T18506 compound SYBR,QPCR
R13968 T18505 T18506 compound Green,QPCR
R13969 T18506 T18508 compound QPCR,mix
R13970 T18507 T18508 compound master,mix
R13971 T18508 T18502 pobj mix,with
R13972 T18509 T18508 punct (,mix
R13973 T18510 T18508 appos Stratagene,mix
R13974 T18511 T18501 punct ),performed
R13975 T18512 T18501 cc and,performed
R13976 T18513 T18515 det the,primers
R13977 T18514 T18515 amod following,primers
R13978 T18515 T18501 conj primers,performed
R13979 T18516 T18515 punct :,primers
R13980 T18517 T18518 compound Ubiquitin,C
R13981 T18518 T18515 appos C,primers
R13982 T18519 T18518 punct ",",C
R13983 T18520 T18531 nummod 5,′
R13984 T18521 T18531 punct ′,′
R13985 T18522 T18531 punct -,′
R13986 T18523 T18531 compound AGG,′
R13987 T18524 T18527 compound TCA,AAG
R13988 T18525 T18527 compound AAC,AAG
R13989 T18526 T18527 compound AGG,AAG
R13990 T18527 T18531 compound AAG,′
R13991 T18528 T18531 compound ACA,′
R13992 T18529 T18531 compound GAC,′
R13993 T18530 T18531 compound GTA-3,′
R13994 T18531 T18518 appos ′,C
R13995 T18532 T18531 cc and,′
R13996 T18533 T18537 nummod 5,′
R13997 T18534 T18536 punct ′,TCACACCCAAGAACAAGCACA-3
R13998 T18535 T18536 punct -,TCACACCCAAGAACAAGCACA-3
R13999 T18536 T18537 compound TCACACCCAAGAACAAGCACA-3,′
R14000 T18537 T18531 conj ′,′
R14001 T18538 T18518 punct ;,C
R14002 T18539 T18518 conj Smad-7,C
R14003 T18540 T18539 punct ",",Smad-7
R14004 T18541 T18551 nummod 5,′
R14005 T18542 T18544 punct ′,GAA
R14006 T18543 T18544 punct -,GAA
R14007 T18544 T18551 compound GAA,′
R14008 T18545 T18549 compound ACC,AAT
R14009 T18546 T18549 compound GGG,AAT
R14010 T18547 T18549 compound GGA,AAT
R14011 T18548 T18549 compound ACG,AAT
R14012 T18549 T18551 compound AAT,′
R14013 T18550 T18551 compound TAT-3,′
R14014 T18551 T18518 appos ′,C
R14015 T18552 T18551 cc and,′
R14016 T18553 T18556 nummod 5,CGC
R14017 T18554 T18556 punct ′,CGC
R14018 T18555 T18556 punct -,CGC
R14019 T18556 T18515 appos CGC,primers
R14020 T18557 T18560 compound GAG,CCT
R14021 T18558 T18560 compound TCT,CCT
R14022 T18559 T18560 compound TCT,CCT
R14023 T18560 T18556 appos CCT,CGC
R14024 T18561 T18561 ROOT CCC,CCC
R14025 T18562 T18563 amod A-3,′
R14026 T18563 T18561 appos ′,CCC
R14027 T18564 T18561 punct ;,CCC
R14028 T18565 T18561 conj TGF-ß1,CCC
R14029 T18566 T18565 punct ",",TGF-ß1
R14030 T18567 T18578 nummod 5,′
R14031 T18568 T18570 punct ′,TGA
R14032 T18569 T18570 punct -,TGA
R14033 T18570 T18573 compound TGA,TGG
R14034 T18571 T18573 compound CGT,TGG
R14035 T18572 T18573 compound CAC,TGG
R14036 T18573 T18578 compound TGG,′
R14037 T18574 T18578 compound AGT,′
R14038 T18575 T18578 compound TGT,′
R14039 T18576 T18578 compound ACG,′
R14040 T18577 T18578 compound G-3,′
R14041 T18578 T18561 conj ′,CCC
R14042 T18579 T18578 cc and,′
R14043 T18580 T18590 nummod 5,′
R14044 T18581 T18588 punct ′,TGG
R14045 T18582 T18588 punct -,TGG
R14046 T18583 T18588 compound GGT,TGG
R14047 T18584 T18588 compound TCA,TGG
R14048 T18585 T18588 compound TGT,TGG
R14049 T18586 T18588 compound CAT,TGG
R14050 T18587 T18588 compound GGA,TGG
R14051 T18588 T18590 nmod TGG,′
R14052 T18589 T18590 nummod TGC-3,′
R14053 T18590 T18578 conj ′,′
R14054 T18591 T18590 punct .,′
R14055 T18592 T18593 compound Primer,pairs
R14056 T18593 T18595 nsubjpass pairs,evaluated
R14057 T18594 T18595 auxpass were,evaluated
R14058 T18595 T18595 ROOT evaluated,evaluated
R14059 T18596 T18595 prep for,evaluated
R14060 T18597 T18596 pobj integrity,for
R14061 T18598 T18595 agent by,evaluated
R14062 T18599 T18606 nmod analysis,curves
R14063 T18600 T18599 prep of,analysis
R14064 T18601 T18603 det the,plot
R14065 T18602 T18603 compound amplification,plot
R14066 T18603 T18600 pobj plot,of
R14067 T18604 T18603 punct ",",plot
R14068 T18605 T18606 compound dissociation,curves
R14069 T18606 T18598 pobj curves,by
R14070 T18607 T18606 punct ",",curves
R14071 T18608 T18606 cc and,curves
R14072 T18609 T18606 conj efficiency,curves
R14073 T18610 T18609 prep of,efficiency
R14074 T18611 T18612 compound PCR,amplification
R14075 T18612 T18610 pobj amplification,of
R14076 T18613 T18595 punct .,evaluated
R14077 T18614 T18615 compound PCR,conditions
R14078 T18615 T18616 nsubj conditions,were
R14079 T18616 T18616 ROOT were,were
R14080 T18617 T18618 nummod 10,min
R14081 T18618 T18616 attr min,were
R14082 T18619 T18616 prep at,were
R14083 T18620 T18621 nummod 95,°
R14084 T18621 T18622 nummod °,C
R14085 T18622 T18619 pobj C,at
R14086 T18623 T18622 punct ",",C
R14087 T18624 T18616 advcl followed,were
R14088 T18625 T18624 agent by,followed
R14089 T18626 T18627 nummod 40,cycles
R14090 T18627 T18625 pobj cycles,by
R14091 T18628 T18627 prep of,cycles
R14092 T18629 T18628 pobj 15,of
R14093 T18630 T18627 appos s,cycles
R14094 T18631 T18624 prep at,followed
R14095 T18632 T18634 nummod 95,C
R14096 T18633 T18634 nummod °,C
R14097 T18634 T18631 pobj C,at
R14098 T18635 T18624 cc and,followed
R14099 T18636 T18637 nummod 60,°
R14100 T18637 T18638 compound °,C
R14101 T18638 T18624 conj C,followed
R14102 T18639 T18638 prep for,C
R14103 T18640 T18641 nummod 1,min
R14104 T18641 T18639 pobj min,for
R14105 T18642 T18616 advcl using,were
R14106 T18643 T18647 det an,system
R14107 T18644 T18647 nummod 7300,system
R14108 T18645 T18647 amod real-time,system
R14109 T18646 T18647 compound PCR,system
R14110 T18647 T18642 dobj system,using
R14111 T18648 T18647 punct (,system
R14112 T18649 T18650 compound Applied,Biosystems
R14113 T18650 T18647 appos Biosystems,system
R14114 T18651 T18647 punct ),system
R14115 T18652 T18616 punct .,were
R14116 T18653 T18654 compound PCR,amplification
R14117 T18654 T18663 nsubjpass amplification,performed
R14118 T18655 T18654 prep of,amplification
R14119 T18656 T18658 det the,gene
R14120 T18657 T18658 compound housekeeping,gene
R14121 T18658 T18655 pobj gene,of
R14122 T18659 T18661 compound encoding,C
R14123 T18660 T18661 compound ubiquitin,C
R14124 T18661 T18663 nsubjpass C,performed
R14125 T18662 T18663 auxpass was,performed
R14126 T18663 T18663 ROOT performed,performed
R14127 T18664 T18663 prep during,performed
R14128 T18665 T18666 det each,run
R14129 T18666 T18664 pobj run,during
R14130 T18667 T18666 prep for,run
R14131 T18668 T18669 det each,sample
R14132 T18669 T18667 pobj sample,for
R14133 T18670 T18671 aux to,allow
R14134 T18671 T18663 advcl allow,performed
R14135 T18672 T18671 dobj normalization,allow
R14136 T18673 T18672 prep between,normalization
R14137 T18674 T18673 pobj samples,between
R14138 T18675 T18663 punct .,performed
R14139 T18676 T18677 compound Relative,quantification
R14140 T18677 T18686 nsubjpass quantification,performed
R14141 T18678 T18677 cc and,quantification
R14142 T18679 T18677 conj calculation,quantification
R14143 T18680 T18679 prep of,calculation
R14144 T18681 T18682 det the,range
R14145 T18682 T18680 pobj range,of
R14146 T18683 T18682 prep of,range
R14147 T18684 T18683 pobj confidence,of
R14148 T18685 T18686 auxpass was,performed
R14149 T18686 T18686 ROOT performed,performed
R14150 T18687 T18686 advcl using,performed
R14151 T18688 T18691 det the,method
R14152 T18689 T18691 amod comparative,method
R14153 T18690 T18691 compound ΔΔCT,method
R14154 T18691 T18687 dobj method,using
R14155 T18692 T18686 punct .,performed
R14254 T18896 T18899 amod Inducible,culture
R14255 T18897 T18898 compound murine,Treg
R14256 T18898 T18899 compound Treg,culture
R14257 T18899 T18899 ROOT culture,culture
R14258 T18900 T18899 punct .,culture
R14259 T18901 T18905 amod Naive,cells
R14260 T18902 T18905 nmod CD4,cells
R14261 T18903 T18905 nummod +,cells
R14262 T18904 T18905 compound T,cells
R14263 T18905 T18916 nsubj cells,were
R14264 T18906 T18905 punct (,cells
R14265 T18907 T18908 nummod CD4,+
R14266 T18908 T18905 appos +,cells
R14267 T18909 T18908 punct ",",+
R14268 T18910 T18911 compound CD62L,+
R14269 T18911 T18908 appos +,+
R14270 T18912 T18911 punct ",",+
R14271 T18913 T18911 cc and,+
R14272 T18914 T18911 conj CD25,+
R14273 T18915 T18905 punct ),cells
R14274 T18916 T18916 ROOT were,were
R14275 T18917 T18916 acomp isolated,were
R14276 T18918 T18917 prep from,isolated
R14277 T18919 T18921 amod pooled,nodes
R14278 T18920 T18921 compound lymph,nodes
R14279 T18921 T18918 pobj nodes,from
R14280 T18922 T18921 cc and,nodes
R14281 T18923 T18921 conj spleens,nodes
R14282 T18924 T18917 agent by,isolated
R14283 T18925 T18924 pobj FACS,by
R14284 T18926 T18928 punct (,Aria
R14285 T18927 T18928 compound FACS,Aria
R14286 T18928 T18916 npadvmod Aria,were
R14287 T18929 T18928 punct ",",Aria
R14288 T18930 T18931 compound BD,Biosciences
R14289 T18931 T18928 appos Biosciences,Aria
R14290 T18932 T18928 punct ),Aria
R14291 T18933 T18916 punct .,were
R14292 T18934 T18936 quantmod 5,105
R14293 T18935 T18936 quantmod ×,105
R14294 T18936 T18938 nummod 105,cells
R14295 T18937 T18938 compound T,cells
R14296 T18938 T18940 nsubjpass cells,co-cultured
R14297 T18939 T18940 auxpass were,co-cultured
R14298 T18940 T18940 ROOT co-cultured,co-cultured
R14299 T18941 T18940 prep with,co-cultured
R14300 T18942 T18944 quantmod 2.5,104
R14301 T18943 T18944 quantmod ×,104
R14302 T18944 T18946 nummod 104,marrow
R14303 T18945 T18946 compound bone,marrow
R14304 T18946 T18941 pobj marrow,with
R14305 T18947 T18949 amod derived,cells
R14306 T18948 T18949 amod dendritic,cells
R14307 T18949 T18946 appos cells,marrow
R14308 T18950 T18952 nmod [,]
R14309 T18951 T18952 nummod 62,]
R14310 T18952 T18949 appos ],cells
R14311 T18953 T18952 cc and,]
R14312 T18954 T18958 compound 0.01,OVA323
R14313 T18955 T18958 compound μg,OVA323
R14314 T18956 T18958 compound /,OVA323
R14315 T18957 T18958 compound ml,OVA323
R14316 T18958 T18952 conj OVA323,]
R14317 T18959 T18960 nummod 339,peptide
R14318 T18960 T18949 appos peptide,cells
R14319 T18961 T18962 punct (,Ansynth
R14320 T18962 T18960 appos Ansynth,peptide
R14321 T18963 T18949 punct ),cells
R14322 T18964 T18946 prep in,marrow
R14323 T18965 T18966 det the,presence
R14324 T18966 T18964 pobj presence,in
R14325 T18967 T18966 cc or,presence
R14326 T18968 T18966 conj absence,presence
R14327 T18969 T18968 prep of,absence
R14328 T18970 T18973 amod 20-ng,rhTGF-ß1
R14329 T18971 T18973 compound /,rhTGF-ß1
R14330 T18972 T18973 compound μl,rhTGF-ß1
R14331 T18973 T18969 pobj rhTGF-ß1,of
R14332 T18974 T18975 punct (,Peprotech
R14333 T18975 T18973 appos Peprotech,rhTGF-ß1
R14334 T18976 T18975 punct ),Peprotech
R14335 T18977 T18966 prep in,presence
R14336 T18978 T18979 amod 48-well,plates
R14337 T18979 T18977 pobj plates,in
R14338 T18980 T18940 punct .,co-cultured
R14339 T18981 T18987 mark After,harvested
R14340 T18982 T18987 meta 4,harvested
R14341 T18983 T18982 advmod d,4
R14342 T18984 T18987 punct ",",harvested
R14343 T18985 T18987 nsubjpass cells,harvested
R14344 T18986 T18987 auxpass were,harvested
R14345 T18987 T18987 ROOT harvested,harvested
R14346 T18988 T18987 cc and,harvested
R14347 T18989 T18987 conj analyzed,harvested
R14348 T18990 T18989 prep for,analyzed
R14349 T18991 T18993 amod intracellular,expression
R14350 T18992 T18993 nummod FOXP3,expression
R14351 T18993 T18990 pobj expression,for
R14352 T18994 T18993 prep by,expression
R14353 T18995 T18994 pobj FACS,by
R14354 T18996 T18995 cc or,FACS
R14355 T18997 T18998 compound gene,expression
R14356 T18998 T18993 conj expression,expression
R14357 T18999 T18993 prep by,expression
R14358 T19000 T19001 amod quantitative,RT-PCR
R14359 T19001 T18999 pobj RT-PCR,by
R14360 T19002 T18987 punct .,harvested
R14484 T19255 T19255 ROOT In,In
R14485 T19256 T19259 amod vitro,differentiation
R14486 T19257 T19259 compound T,differentiation
R14487 T19258 T19259 compound cell,differentiation
R14488 T19259 T19255 pobj differentiation,In
R14489 T19260 T19255 punct .,In
R14490 T19261 T19265 nummod CD4,magnetically-sorted
R14491 T19262 T19265 nummod +,magnetically-sorted
R14492 T19263 T19265 compound CD45RA,magnetically-sorted
R14493 T19264 T19265 compound +,magnetically-sorted
R14494 T19265 T19272 meta magnetically-sorted,according
R14495 T19266 T19265 punct (,magnetically-sorted
R14496 T19267 T19268 nummod CD45RO,depletion
R14497 T19268 T19265 appos depletion,magnetically-sorted
R14498 T19269 T19268 punct ",",depletion
R14499 T19270 T19268 appos MACS,depletion
R14500 T19271 T19265 punct ",",magnetically-sorted
R14501 T19272 T19282 prep according,stimulated
R14502 T19273 T19272 prep to,according
R14503 T19274 T19275 det the,protocol
R14504 T19275 T19273 pobj protocol,to
R14505 T19276 T19275 prep of,protocol
R14506 T19277 T19278 det the,manufacturer
R14507 T19278 T19276 pobj manufacturer,of
R14508 T19279 T19282 punct ),stimulated
R14509 T19280 T19282 nsubjpass cells,stimulated
R14510 T19281 T19282 auxpass were,stimulated
R14511 T19282 T19282 ROOT stimulated,stimulated
R14512 T19283 T19282 prep with,stimulated
R14513 T19284 T19286 amod immobilized,anti-CD3
R14514 T19285 T19286 amod plate-bound,anti-CD3
R14515 T19286 T19283 pobj anti-CD3,with
R14516 T19287 T19291 punct (,ml
R14517 T19288 T19289 nummod 1,μg
R14518 T19289 T19291 compound μg,ml
R14519 T19290 T19291 compound /,ml
R14520 T19291 T19286 appos ml,anti-CD3
R14521 T19292 T19291 punct ",",ml
R14522 T19293 T19291 conj Okt3,ml
R14523 T19294 T19293 punct ",",Okt3
R14524 T19295 T19293 appos IgG1,Okt3
R14525 T19296 T19293 punct ),Okt3
R14526 T19297 T19293 cc and,Okt3
R14527 T19298 T19293 conj anti-CD28,Okt3
R14528 T19299 T19303 punct (,ml
R14529 T19300 T19301 nummod 2,μg
R14530 T19301 T19303 nmod μg,ml
R14531 T19302 T19303 compound /,ml
R14532 T19303 T19298 appos ml,anti-CD28
R14533 T19304 T19303 punct ),ml
R14534 T19305 T19298 prep in,anti-CD28
R14535 T19306 T19307 amod Th1,conditions
R14536 T19307 T19305 pobj conditions,in
R14537 T19308 T19291 punct :,ml
R14538 T19309 T19311 nummod 25,IL-12
R14539 T19310 T19311 compound ng/ml,IL-12
R14540 T19311 T19291 appos IL-12,ml
R14541 T19312 T19311 punct ",",IL-12
R14542 T19313 T19317 nummod 5,anti-IL-4
R14543 T19314 T19317 nmod μg,anti-IL-4
R14544 T19315 T19317 nmod /,anti-IL-4
R14545 T19316 T19317 compound ml,anti-IL-4
R14546 T19317 T19311 appos anti-IL-4,IL-12
R14547 T19318 T19320 punct (,systems
R14548 T19319 T19320 compound R&D,systems
R14549 T19320 T19317 appos systems,anti-IL-4
R14550 T19321 T19320 punct ),systems
R14551 T19322 T19282 punct ;,stimulated
R14552 T19323 T19323 ROOT in,in
R14553 T19324 T19325 amod Th2,conditions
R14554 T19325 T19323 pobj conditions,in
R14555 T19326 T19323 punct :,in
R14556 T19327 T19329 nummod 25,IL-4
R14557 T19328 T19329 compound ng/ml,IL-4
R14558 T19329 T19329 ROOT IL-4,IL-4
R14559 T19330 T19329 punct ",",IL-4
R14560 T19331 T19332 nummod 5,μg
R14561 T19332 T19329 appos μg,IL-4
R14562 T19333 T19335 nmod /,anti-IFN-γ
R14563 T19334 T19335 compound ml,anti-IFN-γ
R14564 T19335 T19332 appos anti-IFN-γ,μg
R14565 T19336 T19335 punct ",",anti-IFN-γ
R14566 T19337 T19338 nummod 5,μg
R14567 T19338 T19341 nmod μg,anti-IL-12
R14568 T19339 T19341 nmod /,anti-IL-12
R14569 T19340 T19341 compound ml,anti-IL-12
R14570 T19341 T19335 appos anti-IL-12,anti-IFN-γ
R14571 T19342 T19344 punct (,systems
R14572 T19343 T19344 compound R&D,systems
R14573 T19344 T19341 appos systems,anti-IL-12
R14574 T19345 T19344 punct ),systems
R14575 T19346 T19344 punct ;,systems
R14576 T19347 T19335 cc or,anti-IFN-γ
R14577 T19348 T19335 prep in,anti-IFN-γ
R14578 T19349 T19350 compound Treg,conditions
R14579 T19350 T19348 pobj conditions,in
R14580 T19351 T19332 punct :,μg
R14581 T19352 T19353 nummod 10,ng/ml
R14582 T19353 T19354 compound ng/ml,TGF-β
R14583 T19354 T19332 appos TGF-β,μg
R14584 T19355 T19354 punct ",",TGF-β
R14585 T19356 T19357 nummod 5,μg
R14586 T19357 T19360 compound μg,anti-IFN-γ
R14587 T19358 T19360 compound /,anti-IFN-γ
R14588 T19359 T19360 compound ml,anti-IFN-γ
R14589 T19360 T19354 appos anti-IFN-γ,TGF-β
R14590 T19361 T19360 punct ",",anti-IFN-γ
R14591 T19362 T19363 nummod 5,μg
R14592 T19363 T19366 nmod μg,anti-IL-12
R14593 T19364 T19366 nmod /,anti-IL-12
R14594 T19365 T19366 compound ml,anti-IL-12
R14595 T19366 T19360 appos anti-IL-12,anti-IFN-γ
R14596 T19367 T19366 punct ",",anti-IL-12
R14597 T19368 T19369 nummod 5,μg
R14598 T19369 T19372 nmod μg,anti-IL-4
R14599 T19370 T19372 nmod /,anti-IL-4
R14600 T19371 T19372 compound ml,anti-IL-4
R14601 T19372 T19366 appos anti-IL-4,anti-IL-12
R14602 T19373 T19282 punct .,stimulated
R14603 T19374 T19375 amod Proliferating,cells
R14604 T19375 T19377 nsubjpass cells,expanded
R14605 T19376 T19377 auxpass were,expanded
R14606 T19377 T19377 ROOT expanded,expanded
R14607 T19378 T19377 prep in,expanded
R14608 T19379 T19380 npadvmod medium,containing
R14609 T19380 T19378 pobj containing,in
R14610 T19381 T19380 dobj IL-2,containing
R14611 T19382 T19381 punct (,IL-2
R14612 T19383 T19384 nummod 30,ng/ml
R14613 T19384 T19381 appos ng/ml,IL-2
R14614 T19385 T19381 punct ),IL-2
R14615 T19386 T19377 punct .,expanded
R14706 T19618 T19618 ROOT Cloning,Cloning
R14707 T19619 T19618 prep of,Cloning
R14708 T19620 T19622 det the,promoter
R14709 T19621 T19622 nummod FOXP3,promoter
R14710 T19622 T19619 pobj promoter,of
R14711 T19623 T19622 cc and,promoter
R14712 T19624 T19622 conj construction,promoter
R14713 T19625 T19622 prep of,promoter
R14714 T19626 T19627 amod mutant,constructs
R14715 T19627 T19625 pobj constructs,of
R14716 T19628 T19618 punct .,Cloning
R14717 T19629 T19631 det The,promoter
R14718 T19630 T19631 nummod FOXP3,promoter
R14719 T19631 T19633 nsubjpass promoter,cloned
R14720 T19632 T19633 auxpass was,cloned
R14721 T19633 T19633 ROOT cloned,cloned
R14722 T19634 T19633 prep into,cloned
R14723 T19635 T19638 det the,vector
R14724 T19636 T19638 amod pGL3,vector
R14725 T19637 T19638 amod basic,vector
R14726 T19638 T19634 pobj vector,into
R14727 T19639 T19638 punct (,vector
R14728 T19640 T19641 compound Promega,Biotech
R14729 T19641 T19638 appos Biotech,vector
R14730 T19642 T19638 punct ),vector
R14731 T19643 T19644 aux to,generate
R14732 T19644 T19633 advcl generate,cloned
R14733 T19645 T19654 det the,]
R14734 T19646 T19654 amod pGL3,]
R14735 T19647 T19654 nummod FOXP3,]
R14736 T19648 T19654 nummod −,]
R14737 T19649 T19650 nummod 511,/
R14738 T19650 T19654 nsubj /,]
R14739 T19651 T19654 nummod +176,]
R14740 T19652 T19654 nmod [,]
R14741 T19653 T19654 nummod 24,]
R14742 T19654 T19644 dobj ],generate
R14743 T19655 T19633 punct .,cloned
R14744 T19656 T19657 amod Site-directed,mutagenesis
R14745 T19657 T19664 nsubjpass mutagenesis,introduced
R14746 T19658 T19657 prep in,mutagenesis
R14747 T19659 T19662 det the,region
R14748 T19660 T19662 nummod FOXP3,region
R14749 T19661 T19662 compound promoter,region
R14750 T19662 T19658 pobj region,in
R14751 T19663 T19664 auxpass were,introduced
R14752 T19664 T19664 ROOT introduced,introduced
R14753 T19665 T19664 advcl using,introduced
R14754 T19666 T19668 det the,kit
R14755 T19667 T19668 compound QuickChange,kit
R14756 T19668 T19665 dobj kit,using
R14757 T19669 T19668 punct (,kit
R14758 T19670 T19668 appos Stratagene,kit
R14759 T19671 T19668 punct ),kit
R14760 T19672 T19664 punct ",",introduced
R14761 T19673 T19664 prep according,introduced
R14762 T19674 T19673 prep to,according
R14763 T19675 T19676 det the,manufacturer
R14764 T19676 T19678 poss manufacturer,instructions
R14765 T19677 T19676 case 's,manufacturer
R14766 T19678 T19674 pobj instructions,to
R14767 T19679 T19664 punct .,introduced
R14768 T19680 T19682 det The,primer
R14769 T19681 T19682 amod following,primer
R14770 T19682 T19688 nsubjpass primer,used
R14771 T19683 T19682 cc and,primer
R14772 T19684 T19686 poss its,strand
R14773 T19685 T19686 amod complementary,strand
R14774 T19686 T19682 conj strand,primer
R14775 T19687 T19688 auxpass were,used
R14776 T19688 T19688 ROOT used,used
R14777 T19689 T19706 punct :,GA.
R14778 T19690 T19691 compound GTT,TCT
R14779 T19691 T19706 compound TCT,GA.
R14780 T19692 T19706 compound CAT,GA.
R14781 T19693 T19699 compound GAG,CTT
R14782 T19694 T19699 compound CCC,CTT
R14783 T19695 T19699 compound TAT,CTT
R14784 T19696 T19699 compound TAA,CTT
R14785 T19697 T19699 compound GTC,CTT
R14786 T19698 T19699 compound ATT,CTT
R14787 T19699 T19706 compound CTT,GA.
R14788 T19700 T19706 compound ACC,GA.
R14789 T19701 T19706 compound TCT,GA.
R14790 T19702 T19706 compound CAC,GA.
R14791 T19703 T19706 compound CTC,GA.
R14792 T19704 T19706 compound TGT,GA.
R14793 T19705 T19706 compound GGT,GA.
R14794 T19706 T19688 npadvmod GA.,used
R14901 T19885 T19885 ROOT Transfections,Transfections
R14902 T19886 T19885 cc and,Transfections
R14903 T19887 T19889 compound reporter,assays
R14904 T19888 T19889 compound gene,assays
R14905 T19889 T19885 conj assays,Transfections
R14906 T19890 T19885 punct .,Transfections
R14907 T19891 T19892 compound T,cells
R14908 T19892 T19894 nsubjpass cells,rested
R14909 T19893 T19894 auxpass were,rested
R14910 T19894 T19894 ROOT rested,rested
R14911 T19895 T19894 prep in,rested
R14912 T19896 T19898 amod serum-free,medium
R14913 T19897 T19898 compound AIM-V,medium
R14914 T19898 T19895 pobj medium,in
R14915 T19899 T19901 punct (,Technologies
R14916 T19900 T19901 compound Life,Technologies
R14917 T19901 T19898 appos Technologies,medium
R14918 T19902 T19898 punct ),medium
R14919 T19903 T19894 advmod overnight,rested
R14920 T19904 T19894 punct .,rested
R14921 T19905 T19906 nummod 3.5,μg
R14922 T19906 T19919 nsubjpass μg,added
R14923 T19907 T19906 prep of,μg
R14924 T19908 T19913 det the,vector
R14925 T19909 T19913 nummod FOXP3,vector
R14926 T19910 T19913 nmod promoter,vector
R14927 T19911 T19913 nmod luciferase,vector
R14928 T19912 T19913 compound reporter,vector
R14929 T19913 T19907 pobj vector,of
R14930 T19914 T19906 cc and,μg
R14931 T19915 T19916 nummod 0.5,μg
R14932 T19916 T19917 compound μg,phRL-TK
R14933 T19917 T19906 conj phRL-TK,μg
R14934 T19918 T19919 auxpass were,added
R14935 T19919 T19919 ROOT added,added
R14936 T19920 T19919 prep to,added
R14937 T19921 T19923 quantmod 3,106
R14938 T19922 T19923 quantmod ×,106
R14939 T19923 T19927 nummod 106,cells
R14940 T19924 T19927 nmod CD4,cells
R14941 T19925 T19927 nummod +,cells
R14942 T19926 T19927 compound T,cells
R14943 T19927 T19920 pobj cells,to
R14944 T19928 T19919 oprd resuspended,added
R14945 T19929 T19928 prep in,resuspended
R14946 T19930 T19931 nummod 100,μL
R14947 T19931 T19929 pobj μL,in
R14948 T19932 T19931 prep of,μL
R14949 T19933 T19934 compound Nucleofector,solution
R14950 T19934 T19932 pobj solution,of
R14951 T19935 T19937 punct (,Biosystems
R14952 T19936 T19937 compound Amaxa,Biosystems
R14953 T19937 T19934 appos Biosystems,solution
R14954 T19938 T19937 punct ),Biosystems
R14955 T19939 T19928 cc and,resuspended
R14956 T19940 T19928 conj electroporated,resuspended
R14957 T19941 T19940 xcomp using,electroporated
R14958 T19942 T19944 det the,program
R14959 T19943 T19944 compound U-15,program
R14960 T19944 T19941 dobj program,using
R14961 T19945 T19944 prep of,program
R14962 T19946 T19947 det the,Nucleofector
R14963 T19947 T19945 pobj Nucleofector,of
R14964 T19948 T19919 punct .,added
R14965 T19949 T19967 prep After,measured
R14966 T19950 T19952 det a,culture
R14967 T19951 T19952 amod 24-h,culture
R14968 T19952 T19949 pobj culture,After
R14969 T19953 T19952 prep in,culture
R14970 T19954 T19955 amod serum-free,conditions
R14971 T19955 T19953 pobj conditions,in
R14972 T19956 T19955 cc and,conditions
R14973 T19957 T19955 conj stimuli,conditions
R14974 T19958 T19959 mark as,indicated
R14975 T19959 T19967 advcl indicated,measured
R14976 T19960 T19959 prep in,indicated
R14977 T19961 T19962 det the,figures
R14978 T19962 T19960 pobj figures,in
R14979 T19963 T19967 punct ",",measured
R14980 T19964 T19965 compound luciferase,activity
R14981 T19965 T19967 nsubjpass activity,measured
R14982 T19966 T19967 auxpass was,measured
R14983 T19967 T19967 ROOT measured,measured
R14984 T19968 T19967 agent by,measured
R14985 T19969 T19973 det the,system
R14986 T19970 T19973 amod dual,system
R14987 T19971 T19973 compound luciferase,system
R14988 T19972 T19973 compound assay,system
R14989 T19973 T19968 pobj system,by
R14990 T19974 T19973 punct (,system
R14991 T19975 T19976 compound Promega,Biotech
R14992 T19976 T19973 appos Biotech,system
R14993 T19977 T19973 punct ),system
R14994 T19978 T19967 prep according,measured
R14995 T19979 T19978 prep to,according
R14996 T19980 T19981 det the,manufacturer
R14997 T19981 T19983 poss manufacturer,instructions
R14998 T19982 T19981 case 's,manufacturer
R14999 T19983 T19979 pobj instructions,to
R15000 T19984 T19967 punct .,measured
R15001 T19985 T19987 nsubjpass Data,normalized
R15002 T19986 T19987 auxpass were,normalized
R15003 T19987 T19987 ROOT normalized,normalized
R15004 T19988 T19987 agent by,normalized
R15005 T19989 T19990 det the,activity
R15006 T19990 T19988 pobj activity,by
R15007 T19991 T19990 prep of,activity
R15008 T19992 T19993 compound renilla,luciferase
R15009 T19993 T19991 pobj luciferase,of
R15010 T19994 T19987 punct .,normalized
R15056 T20100 T20101 compound RNA,isolation
R15057 T20101 T20101 ROOT isolation,isolation
R15058 T20102 T20101 cc and,isolation
R15059 T20103 T20104 compound cDNA,synthesis
R15060 T20104 T20101 conj synthesis,isolation
R15061 T20105 T20101 punct .,isolation
R15062 T20106 T20108 nsubjpass RNA,isolated
R15063 T20107 T20108 auxpass was,isolated
R15064 T20108 T20108 ROOT isolated,isolated
R15065 T20109 T20108 advcl using,isolated
R15066 T20110 T20113 det the,Kit
R15067 T20111 T20113 compound RNeasy,Kit
R15068 T20112 T20113 compound Mini,Kit
R15069 T20113 T20109 dobj Kit,using
R15070 T20114 T20115 punct (,Qiagen
R15071 T20115 T20113 appos Qiagen,Kit
R15072 T20116 T20113 punct ),Kit
R15073 T20117 T20108 prep according,isolated
R15074 T20118 T20117 prep to,according
R15075 T20119 T20120 det the,manufacturer
R15076 T20120 T20122 poss manufacturer,protocol
R15077 T20121 T20120 case 's,manufacturer
R15078 T20122 T20118 pobj protocol,to
R15079 T20123 T20108 punct .,isolated
R15080 T20124 T20124 ROOT Reverse,Reverse
R15081 T20125 T20130 nsubjpass transcription,performed
R15082 T20126 T20125 prep of,transcription
R15083 T20127 T20128 amod human,samples
R15084 T20128 T20126 pobj samples,of
R15085 T20129 T20130 auxpass was,performed
R15086 T20130 T20124 ccomp performed,Reverse
R15087 T20131 T20130 prep with,performed
R15088 T20132 T20134 nmod TaqMan,reagents
R15089 T20133 T20134 compound reverse-transcription,reagents
R15090 T20134 T20131 pobj reagents,with
R15091 T20135 T20137 punct (,Biosystems
R15092 T20136 T20137 compound Applied,Biosystems
R15093 T20137 T20134 appos Biosystems,reagents
R15094 T20138 T20130 punct ),performed
R15095 T20139 T20130 prep with,performed
R15096 T20140 T20141 amod random,hexamers
R15097 T20141 T20139 pobj hexamers,with
R15098 T20142 T20124 prep according,Reverse
R15099 T20143 T20142 prep to,according
R15100 T20144 T20145 det the,manufacturer
R15101 T20145 T20147 poss manufacturer,protocol
R15102 T20146 T20145 case 's,manufacturer
R15103 T20147 T20143 pobj protocol,to
R15104 T20148 T20124 punct .,Reverse
R15189 T20272 T20274 amod Recombinant,proteins
R15190 T20273 T20274 compound TAT,proteins
R15191 T20274 T20274 ROOT proteins,proteins
R15192 T20275 T20274 punct .,proteins
R15193 T20276 T20280 det The,protein
R15194 T20277 T20278 nsubj cDNAs,encoding
R15195 T20278 T20280 amod encoding,protein
R15196 T20279 T20280 nummod GATA3,protein
R15197 T20280 T20293 nsubjpass protein,cloned
R15198 T20281 T20280 cc or,protein
R15199 T20282 T20284 det the,GATA3
R15200 T20283 T20284 amod truncated,GATA3
R15201 T20284 T20280 conj GATA3,protein
R15202 T20285 T20286 punct (,lacking
R15203 T20286 T20284 acl lacking,GATA3
R15204 T20287 T20290 det the,fingers
R15205 T20288 T20290 nummod two,fingers
R15206 T20289 T20290 compound zinc,fingers
R15207 T20290 T20286 dobj fingers,lacking
R15208 T20291 T20290 punct ),fingers
R15209 T20292 T20293 auxpass were,cloned
R15210 T20293 T20293 ROOT cloned,cloned
R15211 T20294 T20293 prep in,cloned
R15212 T20295 T20294 pobj frame,in
R15213 T20296 T20293 prep into,cloned
R15214 T20297 T20299 det an,vector
R15215 T20298 T20299 compound expression,vector
R15216 T20299 T20296 pobj vector,into
R15217 T20300 T20299 prep along,vector
R15218 T20301 T20300 prep with,along
R15219 T20302 T20304 det the,sequence
R15220 T20303 T20304 compound TAT,sequence
R15221 T20304 T20301 pobj sequence,with
R15222 T20305 T20307 mark as,described
R15223 T20306 T20307 advmod previously,described
R15224 T20307 T20293 advcl described,cloned
R15225 T20308 T20310 nmod [,]
R15226 T20309 T20310 nummod 63,]
R15227 T20310 T20307 dobj ],described
R15228 T20311 T20293 punct .,cloned
R15229 T20312 T20314 nsubjpass Proteins,expressed
R15230 T20313 T20314 auxpass were,expressed
R15231 T20314 T20314 ROOT expressed,expressed
R15232 T20315 T20314 prep in,expressed
R15233 T20316 T20317 compound BL21,Star
R15234 T20317 T20315 pobj Star,in
R15235 T20318 T20317 punct (,Star
R15236 T20319 T20317 appos DE3,Star
R15237 T20320 T20317 punct ),Star
R15238 T20321 T20328 nsubjpass pLysS,purified
R15239 T20322 T20323 punct (,Invitrogen
R15240 T20323 T20321 appos Invitrogen,pLysS
R15241 T20324 T20323 punct ),Invitrogen
R15242 T20325 T20321 cc and,pLysS
R15243 T20326 T20321 conj lysates,pLysS
R15244 T20327 T20328 auxpass were,purified
R15245 T20328 T20314 advcl purified,expressed
R15246 T20329 T20328 agent by,purified
R15247 T20330 T20329 pobj Ni2,by
R15248 T20331 T20333 punct +,chelate
R15249 T20332 T20333 punct -,chelate
R15250 T20333 T20334 compound chelate,column
R15251 T20334 T20335 compound column,chromatography
R15252 T20335 T20328 prep chromatography,purified
R15253 T20336 T20314 punct .,expressed
R15254 T20337 T20339 det Both,proteins
R15255 T20338 T20339 amod TAT-linked,proteins
R15256 T20339 T20340 nsubj proteins,were
R15257 T20340 T20340 ROOT were,were
R15258 T20341 T20343 amod more,95
R15259 T20342 T20343 quantmod than,95
R15260 T20343 T20344 nummod 95,%
R15261 T20344 T20345 npadvmod %,pure
R15262 T20345 T20340 acomp pure,were
R15263 T20346 T20340 punct ",",were
R15264 T20347 T20340 prep based,were
R15265 T20348 T20347 prep on,based
R15266 T20349 T20351 nmod Coomassie,staining
R15267 T20350 T20351 amod blue,staining
R15268 T20351 T20348 pobj staining,on
R15269 T20352 T20351 prep of,staining
R15270 T20353 T20356 compound sodium,gels
R15271 T20354 T20356 compound disulfate,gels
R15272 T20355 T20356 compound acrylamide,gels
R15273 T20356 T20352 pobj gels,of
R15274 T20357 T20340 punct .,were
R15368 T20544 T20545 nummod TAT-GATA3,transduction
R15369 T20545 T20545 ROOT transduction,transduction
R15370 T20546 T20545 punct .,transduction
R15371 T20547 T20551 nummod CD4,cells
R15372 T20548 T20551 nummod +,cells
R15373 T20549 T20551 compound CD45RA,cells
R15374 T20550 T20551 compound +,cells
R15375 T20551 T20552 nsubj cells,were
R15376 T20552 T20552 ROOT were,were
R15377 T20553 T20552 acomp cultured,were
R15378 T20554 T20553 prep in,cultured
R15379 T20555 T20556 compound AIMV,medium
R15380 T20556 T20554 pobj medium,in
R15381 T20557 T20556 cc and,medium
R15382 T20558 T20552 prep transduced,were
R15383 T20559 T20558 prep with,transduced
R15384 T20560 T20561 nummod 20,nM
R15385 T20561 T20559 pobj nM,with
R15386 T20562 T20561 punct ",",nM
R15387 T20563 T20564 nummod 10,nM
R15388 T20564 T20561 conj nM,nM
R15389 T20565 T20564 punct ",",nM
R15390 T20566 T20564 cc or,nM
R15391 T20567 T20568 nummod 500,nM
R15392 T20568 T20564 conj nM,nM
R15393 T20569 T20568 prep of,nM
R15394 T20570 T20573 amod full-length,GATA3
R15395 T20571 T20570 cc or,full-length
R15396 T20572 T20570 conj truncated,full-length
R15397 T20573 T20569 pobj GATA3,of
R15398 T20574 T20568 prep over,nM
R15399 T20575 T20576 det the,course
R15400 T20576 T20574 pobj course,over
R15401 T20577 T20576 prep of,course
R15402 T20578 T20579 nummod 4,h
R15403 T20579 T20577 pobj h,of
R15404 T20580 T20552 punct .,were
R15405 T20581 T20588 prep After,washed
R15406 T20582 T20583 nummod 4,h
R15407 T20583 T20581 pobj h,After
R15408 T20584 T20588 punct ",",washed
R15409 T20585 T20586 det the,cells
R15410 T20586 T20588 nsubjpass cells,washed
R15411 T20587 T20588 auxpass were,washed
R15412 T20588 T20588 ROOT washed,washed
R15413 T20589 T20588 cc and,washed
R15414 T20590 T20588 conj activated,washed
R15415 T20591 T20590 prep with,activated
R15416 T20592 T20593 amod soluble,anti-CD3
R15417 T20593 T20591 pobj anti-CD3,with
R15418 T20594 T20593 cc and,anti-CD3
R15419 T20595 T20593 conj anti-CD28,anti-CD3
R15420 T20596 T20595 cc and,anti-CD28
R15421 T20597 T20595 conj TGF-β,anti-CD28
R15422 T20598 T20600 punct (,ng/ml
R15423 T20599 T20600 nummod 10,ng/ml
R15424 T20600 T20597 appos ng/ml,TGF-β
R15425 T20601 T20600 punct ),ng/ml
R15426 T20602 T20588 punct .,washed
R15427 T20603 T20604 det Each,day
R15428 T20604 T20611 npadvmod day,added
R15429 T20605 T20611 punct ",",added
R15430 T20606 T20608 det the,proteins
R15431 T20607 T20608 compound TAT,proteins
R15432 T20608 T20611 nsubjpass proteins,added
R15433 T20609 T20611 auxpass were,added
R15434 T20610 T20611 advmod freshly,added
R15435 T20611 T20611 ROOT added,added
R15436 T20612 T20611 prep to,added
R15437 T20613 T20614 det the,medium
R15438 T20614 T20612 pobj medium,to
R15439 T20615 T20611 punct .,added
R15440 T20616 T20617 nummod FOXP3,expression
R15441 T20617 T20619 nsubjpass expression,measured
R15442 T20618 T20619 auxpass was,measured
R15443 T20619 T20619 ROOT measured,measured
R15444 T20620 T20619 prep after,measured
R15445 T20621 T20622 nummod 5,d
R15446 T20622 T20620 pobj d,after
R15447 T20623 T20622 prep by,d
R15448 T20624 T20625 amod intracellular,staining
R15449 T20625 T20623 pobj staining,by
R15450 T20626 T20619 punct .,measured
R15530 T20794 T20796 amod Intracellular,staining
R15531 T20795 T20796 compound cytokine,staining
R15532 T20796 T20796 ROOT staining,staining
R15533 T20797 T20796 punct .,staining
R15534 T20798 T20799 compound T,cells
R15535 T20799 T20801 nsubjpass cells,stimulated
R15536 T20800 T20801 auxpass were,stimulated
R15537 T20801 T20801 ROOT stimulated,stimulated
R15538 T20802 T20801 prep with,stimulated
R15539 T20803 T20807 quantmod 2,7
R15540 T20804 T20807 quantmod ×,7
R15541 T20805 T20807 quantmod 10,7
R15542 T20806 T20807 quantmod −,7
R15543 T20807 T20809 nummod 7,PMA
R15544 T20808 T20809 compound M,PMA
R15545 T20809 T20802 pobj PMA,with
R15546 T20810 T20809 cc and,PMA
R15547 T20811 T20812 nummod 1,μg
R15548 T20812 T20814 compound μg,ml
R15549 T20813 T20814 compound /,ml
R15550 T20814 T20809 conj ml,PMA
R15551 T20815 T20814 prep of,ml
R15552 T20816 T20815 pobj ionomycin,of
R15553 T20817 T20819 punct (,Chemicals
R15554 T20818 T20819 compound Sigma,Chemicals
R15555 T20819 T20816 parataxis Chemicals,ionomycin
R15556 T20820 T20814 punct ),ml
R15557 T20821 T20801 prep for,stimulated
R15558 T20822 T20823 nummod 4,h
R15559 T20823 T20821 pobj h,for
R15560 T20824 T20801 punct .,stimulated
R15561 T20825 T20827 det The,mAb
R15562 T20826 T20827 amod following,mAb
R15563 T20827 T20829 nsubjpass mAb,used
R15564 T20828 T20829 auxpass was,used
R15565 T20829 T20829 ROOT used,used
R15566 T20830 T20829 punct :,used
R15567 T20831 T20838 meta anti-IL-4-PE,.
R15568 T20832 T20831 punct (,anti-IL-4-PE
R15569 T20833 T20834 nummod 8D4,8
R15570 T20834 T20831 appos 8,anti-IL-4-PE
R15571 T20835 T20831 punct ",",anti-IL-4-PE
R15572 T20836 T20831 appos BD,anti-IL-4-PE
R15573 T20837 T20831 punct ),anti-IL-4-PE
R15574 T20838 T20829 punct .,used
R15575 T20839 T20841 amod Matched,controls
R15576 T20840 T20841 compound isotype,controls
R15577 T20841 T20843 nsubjpass controls,used
R15578 T20842 T20843 auxpass were,used
R15579 T20843 T20843 ROOT used,used
R15580 T20844 T20843 prep at,used
R15581 T20845 T20848 det the,concentration
R15582 T20846 T20848 amod same,concentration
R15583 T20847 T20848 compound protein,concentration
R15584 T20848 T20844 pobj concentration,at
R15585 T20849 T20848 prep as,concentration
R15586 T20850 T20852 det the,antibodies
R15587 T20851 T20852 amod respective,antibodies
R15588 T20852 T20849 pobj antibodies,as
R15589 T20853 T20843 punct .,used
R15590 T20854 T20855 compound Four-color,FACS
R15591 T20855 T20857 nsubjpass FACS,performed
R15592 T20856 T20857 auxpass was,performed
R15593 T20857 T20857 ROOT performed,performed
R15594 T20858 T20857 advcl using,performed
R15595 T20859 T20861 det an,XL-MCL
R15596 T20860 T20861 compound EPICS,XL-MCL
R15597 T20861 T20858 dobj XL-MCL,using
R15598 T20862 T20864 punct (,Coulter
R15599 T20863 T20864 compound Beckman,Coulter
R15600 T20864 T20861 appos Coulter,XL-MCL
R15601 T20865 T20861 punct ),XL-MCL
R15602 T20866 T20857 advcl using,performed
R15603 T20867 T20870 det the,version
R15604 T20868 T20870 compound software,version
R15605 T20869 T20870 compound Expo32,version
R15606 T20870 T20866 dobj version,using
R15607 T20871 T20870 prep for,version
R15608 T20872 T20873 compound data,acquisition
R15609 T20873 T20871 pobj acquisition,for
R15610 T20874 T20873 cc and,acquisition
R15611 T20875 T20873 conj evaluation,acquisition
R15612 T20876 T20857 punct .,performed
R15901 T21273 T21274 compound Flow,cytometry
R15902 T21274 T21274 ROOT cytometry,cytometry
R15903 T21275 T21274 punct .,cytometry
R15904 T21276 T21289 prep For,stained
R15905 T21277 T21276 pobj analysis,For
R15906 T21278 T21277 prep of,analysis
R15907 T21279 T21280 nummod FOXP3,expression
R15908 T21280 T21278 pobj expression,of
R15909 T21281 T21280 prep at,expression
R15910 T21282 T21284 det the,level
R15911 T21283 T21284 amod single-cell,level
R15912 T21284 T21281 pobj level,at
R15913 T21285 T21289 punct ",",stained
R15914 T21286 T21289 nsubjpass cells,stained
R15915 T21287 T21289 auxpass were,stained
R15916 T21288 T21289 advmod first,stained
R15917 T21289 T21289 ROOT stained,stained
R15918 T21290 T21289 prep with,stained
R15919 T21291 T21294 det the,CD25
R15920 T21292 T21294 amod monoclonal,CD25
R15921 T21293 T21294 compound antibody,CD25
R15922 T21294 T21290 pobj CD25,with
R15923 T21295 T21294 punct (,CD25
R15924 T21296 T21297 compound Beckman,Coulter
R15925 T21297 T21294 appos Coulter,CD25
R15926 T21298 T21294 punct ),CD25
R15927 T21299 T21289 punct ",",stained
R15928 T21300 T21289 cc and,stained
R15929 T21301 T21308 prep after,incubated
R15930 T21302 T21301 pobj fixation,after
R15931 T21303 T21302 cc and,fixation
R15932 T21304 T21302 conj permeabilization,fixation
R15933 T21305 T21308 punct ",",incubated
R15934 T21306 T21308 nsubjpass cells,incubated
R15935 T21307 T21308 auxpass were,incubated
R15936 T21308 T21289 conj incubated,stained
R15937 T21309 T21308 prep with,incubated
R15938 T21310 T21313 amod PE-conjugated,PCH101
R15939 T21311 T21312 amod monoclonal,antibody
R15940 T21312 T21313 compound antibody,PCH101
R15941 T21313 T21309 pobj PCH101,with
R15942 T21314 T21313 punct (,PCH101
R15943 T21315 T21316 amod anti-human,FOXP3
R15944 T21316 T21313 npadvmod FOXP3,PCH101
R15945 T21317 T21313 punct ;,PCH101
R15946 T21318 T21313 conj eBioscience,PCH101
R15947 T21319 T21308 punct ),incubated
R15948 T21320 T21308 prep based,incubated
R15949 T21321 T21320 prep on,based
R15950 T21322 T21323 det the,manufacturer
R15951 T21323 T21325 poss manufacturer,recommendations
R15952 T21324 T21323 case 's,manufacturer
R15953 T21325 T21321 pobj recommendations,on
R15954 T21326 T21320 cc and,based
R15955 T21327 T21320 conj subjected,based
R15956 T21328 T21327 prep to,subjected
R15957 T21329 T21328 pobj FACS,to
R15958 T21330 T21332 punct (,XL-MCL
R15959 T21331 T21332 compound EPICS,XL-MCL
R15960 T21332 T21329 appos XL-MCL,FACS
R15961 T21333 T21332 punct ),XL-MCL
R15962 T21334 T21308 punct .,incubated
R15963 T21335 T21343 prep For,incubated
R15964 T21336 T21337 compound cell,surface
R15965 T21337 T21338 compound surface,marker
R15966 T21338 T21339 compound marker,staining
R15967 T21339 T21335 pobj staining,For
R15968 T21340 T21343 punct ",",incubated
R15969 T21341 T21343 nsubjpass cells,incubated
R15970 T21342 T21343 auxpass were,incubated
R15971 T21343 T21343 ROOT incubated,incubated
R15972 T21344 T21343 prep for,incubated
R15973 T21345 T21346 nummod 20,min
R15974 T21346 T21344 pobj min,for
R15975 T21347 T21343 prep at,incubated
R15976 T21348 T21349 nummod 4,°
R15977 T21349 T21347 pobj °,at
R15978 T21350 T21343 dobj C,incubated
R15979 T21351 T21343 prep in,incubated
R15980 T21352 T21351 pcomp staining,in
R15981 T21353 T21352 dobj buffer,staining
R15982 T21354 T21352 prep with,staining
R15983 T21355 T21357 det the,antibodies
R15984 T21356 T21357 amod following,antibodies
R15985 T21357 T21354 pobj antibodies,with
R15986 T21358 T21357 punct :,antibodies
R15987 T21359 T21357 appos anti,antibodies
R15988 T21360 T21357 appos CD152-PE,antibodies
R15989 T21361 T21362 punct (,CTLA-4
R15990 T21362 T21360 appos CTLA-4,CD152-PE
R15991 T21363 T21364 punct ;,BD
R15992 T21364 T21343 npadvmod BD,incubated
R15993 T21365 T21364 punct ),BD
R15994 T21366 T21364 punct ",",BD
R15995 T21367 T21368 amod anti,PD-1
R15996 T21368 T21364 appos PD-1,BD
R15997 T21369 T21370 punct (,eBiosciences
R15998 T21370 T21368 appos eBiosciences,PD-1
R15999 T21371 T21368 punct ),PD-1
R16000 T21372 T21368 punct ",",PD-1
R16001 T21373 T21375 amod anti-GITR,R
R16002 T21374 T21375 punct (,R
R16003 T21375 T21364 appos R,BD
R16004 T21376 T21375 cc &,R
R16005 T21377 T21378 compound D,Systems
R16006 T21378 T21375 conj Systems,R
R16007 T21379 T21375 punct ),R
R16008 T21380 T21375 punct ",",R
R16009 T21381 T21375 appos anti-CD69,R
R16010 T21382 T21384 punct (,Coulter
R16011 T21383 T21384 compound Beckman,Coulter
R16012 T21384 T21381 appos Coulter,anti-CD69
R16013 T21385 T21384 punct ),Coulter
R16014 T21386 T21381 punct ",",anti-CD69
R16015 T21387 T21381 appos anti-CD103,anti-CD69
R16016 T21388 T21389 punct (,DakoCytomation
R16017 T21389 T21387 appos DakoCytomation,anti-CD103
R16018 T21390 T21389 punct ),DakoCytomation
R16019 T21391 T21387 punct ",",anti-CD103
R16020 T21392 T21387 conj anti-CD62L,anti-CD103
R16021 T21393 T21395 punct (,Coulter
R16022 T21394 T21395 compound Beckman,Coulter
R16023 T21395 T21392 appos Coulter,anti-CD62L
R16024 T21396 T21395 punct ),Coulter
R16025 T21397 T21395 punct ",",Coulter
R16026 T21398 T21395 cc or,Coulter
R16027 T21399 T21395 conj anti-HLA-DR,Coulter
R16028 T21400 T21402 punct (,Coulter
R16029 T21401 T21402 compound Beckman,Coulter
R16030 T21402 T21399 appos Coulter,anti-HLA-DR
R16031 T21403 T21399 punct ),anti-HLA-DR
R16032 T21404 T21343 punct .,incubated
R16033 T21405 T21406 det The,controls
R16034 T21406 T21407 nsubj controls,were
R16035 T21407 T21407 ROOT were,were
R16036 T21408 T21407 attr FITC,were
R16037 T21409 T21408 punct ",",FITC
R16038 T21410 T21408 conj PE,FITC
R16039 T21411 T21410 punct ",",PE
R16040 T21412 T21410 cc or,PE
R16041 T21413 T21414 compound ECD-conjugated,mouse
R16042 T21414 T21415 compound mouse,IgG1
R16043 T21415 T21410 conj IgG1,PE
R16044 T21416 T21415 cc or,IgG1
R16045 T21417 T21415 conj rat,IgG1
R16046 T21418 T21408 appos IgG2a,FITC
R16047 T21419 T21407 punct .,were
R16048 T21420 T21433 prep For,used
R16049 T21421 T21420 pobj staining,For
R16050 T21422 T21421 prep of,staining
R16051 T21423 T21424 compound mouse,cells
R16052 T21424 T21422 pobj cells,of
R16053 T21425 T21433 punct ",",used
R16054 T21426 T21428 det the,mAbs
R16055 T21427 T21428 amod following,mAbs
R16056 T21428 T21433 nsubjpass mAbs,used
R16057 T21429 T21428 prep from,mAbs
R16058 T21430 T21431 compound BD,Biosciences
R16059 T21431 T21429 pobj Biosciences,from
R16060 T21432 T21433 auxpass were,used
R16061 T21433 T21433 ROOT used,used
R16062 T21434 T21433 prep following,used
R16063 T21435 T21436 compound standard,techniques
R16064 T21436 T21434 pobj techniques,following
R16065 T21437 T21438 mark as,described
R16066 T21438 T21438 ROOT described,described
R16067 T21439 T21438 prep above,described
R16068 T21440 T21438 punct :,described
R16069 T21441 T21433 dobj anti-CD3,used
R16070 T21442 T21441 punct ",",anti-CD3
R16071 T21443 T21441 conj anti-CD4,anti-CD3
R16072 T21444 T21443 punct ",",anti-CD4
R16073 T21445 T21443 cc and,anti-CD4
R16074 T21446 T21443 conj anti-CD25,anti-CD4
R16075 T21447 T21433 punct .,used
R16076 T21448 T21451 compound Anti-FcγRII,antibody
R16077 T21449 T21450 compound /,III
R16078 T21450 T21451 compound III,antibody
R16079 T21451 T21459 nsubjpass antibody,included
R16080 T21452 T21454 punct (,G2
R16081 T21453 T21454 nummod 2.4,G2
R16082 T21454 T21451 appos G2,antibody
R16083 T21455 T21454 punct ",",G2
R16084 T21456 T21454 appos ATCC,G2
R16085 T21457 T21454 punct ),G2
R16086 T21458 T21459 auxpass was,included
R16087 T21459 T21459 ROOT included,included
R16088 T21460 T21459 prep in,included
R16089 T21461 T21462 det all,stainings
R16090 T21462 T21460 pobj stainings,in
R16091 T21463 T21464 aux to,reduce
R16092 T21464 T21459 advcl reduce,included
R16093 T21465 T21467 amod nonspecific,binding
R16094 T21466 T21467 compound antibody,binding
R16095 T21467 T21464 dobj binding,reduce
R16096 T21468 T21459 punct .,included
R16097 T21469 T21470 aux To,isolate
R16098 T21470 T21487 advcl isolate,stained
R16099 T21471 T21472 amod naive,murine
R16100 T21472 T21470 dobj murine,isolate
R16101 T21473 T21475 compound CD4,cells
R16102 T21474 T21475 compound T,cells
R16103 T21475 T21472 appos cells,murine
R16104 T21476 T21475 prep from,cells
R16105 T21477 T21478 compound murine,DO11
R16106 T21478 T21476 pobj DO11,from
R16107 T21479 T21478 nummod .10,DO11
R16108 T21480 T21478 cc or,DO11
R16109 T21481 T21483 amod DO11.10xCD2-GATA3,cells
R16110 T21482 T21483 compound T,cells
R16111 T21483 T21478 conj cells,DO11
R16112 T21484 T21475 punct ",",cells
R16113 T21485 T21487 nsubjpass cells,stained
R16114 T21486 T21487 auxpass were,stained
R16115 T21487 T21487 ROOT stained,stained
R16116 T21488 T21487 prep with,stained
R16117 T21489 T21495 amod anti-CD25-FITC,prior
R16118 T21490 T21489 punct ",",anti-CD25-FITC
R16119 T21491 T21489 conj anti-CD62L-PE,anti-CD25-FITC
R16120 T21492 T21491 punct ",",anti-CD62L-PE
R16121 T21493 T21491 cc and,anti-CD62L-PE
R16122 T21494 T21491 conj anti-CD4-APC,anti-CD62L-PE
R16123 T21495 T21487 advmod prior,stained
R16124 T21496 T21495 prep to,prior
R16125 T21497 T21496 pobj sorting,to
R16126 T21498 T21487 punct .,stained
R16127 T21499 T21500 compound Dead,cells
R16128 T21500 T21502 nsubjpass cells,excluded
R16129 T21501 T21502 auxpass were,excluded
R16130 T21502 T21509 ccomp excluded,Diamidino-2-phenylindole
R16131 T21503 T21502 prep with,excluded
R16132 T21504 T21505 nummod 4,′
R16133 T21505 T21503 pobj ′,with
R16134 T21506 T21505 punct ',′
R16135 T21507 T21509 punct ",6",Diamidino-2-phenylindole
R16136 T21508 T21509 punct -,Diamidino-2-phenylindole
R16137 T21509 T21509 ROOT Diamidino-2-phenylindole,Diamidino-2-phenylindole
R16138 T21510 T21511 punct (,DAPI
R16139 T21511 T21509 appos DAPI,Diamidino-2-phenylindole
R16140 T21512 T21511 punct ),DAPI
R16141 T21513 T21509 punct .,Diamidino-2-phenylindole
R16142 T21514 T21515 aux To,analyze
R16143 T21515 T21526 advcl analyze,stained
R16144 T21516 T21518 amod murine,expression
R16145 T21517 T21518 amod Foxp3,expression
R16146 T21518 T21515 dobj expression,analyze
R16147 T21519 T21518 prep in,expression
R16148 T21520 T21522 amod inducible,cultures
R16149 T21521 T21522 compound Treg,cultures
R16150 T21522 T21519 pobj cultures,in
R16151 T21523 T21526 punct ",",stained
R16152 T21524 T21526 nsubjpass cells,stained
R16153 T21525 T21526 auxpass were,stained
R16154 T21526 T21526 ROOT stained,stained
R16155 T21527 T21526 advmod intracellularly,stained
R16156 T21528 T21526 prep with,stained
R16157 T21529 T21530 amod anti-Foxp3-PE,according
R16158 T21530 T21526 prep according,stained
R16159 T21531 T21530 prep to,according
R16160 T21532 T21534 poss manufacturer,instruction
R16161 T21533 T21532 case 's,manufacturer
R16162 T21534 T21531 pobj instruction,to
R16163 T21535 T21526 punct ",",stained
R16164 T21536 T21526 prep in,stained
R16165 T21537 T21536 pobj conjunction,in
R16166 T21538 T21537 prep with,conjunction
R16167 T21539 T21546 amod anti-CD4-APC,kit
R16168 T21540 T21539 cc and,anti-CD4-APC
R16169 T21541 T21539 conj LIVE/DEAD,anti-CD4-APC
R16170 T21542 T21546 amod fixable,kit
R16171 T21543 T21544 amod dead,cell
R16172 T21544 T21545 compound cell,stain
R16173 T21545 T21546 compound stain,kit
R16174 T21546 T21538 pobj kit,with
R16175 T21547 T21546 punct (,kit
R16176 T21548 T21546 appos Invitrogen,kit
R16177 T21549 T21546 punct ),kit
R16178 T21550 T21551 aux to,discriminate
R16179 T21551 T21526 xcomp discriminate,stained
R16180 T21552 T21553 amod live,cells
R16181 T21553 T21551 dobj cells,discriminate
R16182 T21554 T21526 punct .,stained
R16183 T21555 T21557 det All,antibodies
R16184 T21556 T21557 amod monoclonal,antibodies
R16185 T21557 T21563 nsubjpass antibodies,purchased
R16186 T21558 T21557 prep for,antibodies
R16187 T21559 T21560 amod murine,cell
R16188 T21560 T21561 compound cell,stainings
R16189 T21561 T21558 pobj stainings,for
R16190 T21562 T21563 auxpass were,purchased
R16191 T21563 T21563 ROOT purchased,purchased
R16192 T21564 T21563 prep from,purchased
R16193 T21565 T21564 pobj eBioscience,from
R16194 T21566 T21565 cc or,eBioscience
R16195 T21567 T21568 compound BD,Biosciences
R16196 T21568 T21565 conj Biosciences,eBioscience
R16197 T21569 T21563 punct .,purchased
R16383 T21972 T21973 amod Western,blotting
R16384 T21973 T21973 ROOT blotting,blotting
R16385 T21974 T21973 punct .,blotting
R16386 T21975 T21991 prep For,lysed
R16387 T21976 T21977 nummod FOXP3,analysis
R16388 T21977 T21975 pobj analysis,For
R16389 T21978 T21977 prep on,analysis
R16390 T21979 T21981 det the,level
R16391 T21980 T21981 compound protein,level
R16392 T21981 T21978 pobj level,on
R16393 T21982 T21991 punct ",",lysed
R16394 T21983 T21985 nummod 1,106
R16395 T21984 T21985 nummod ×,106
R16396 T21985 T21988 nummod 106,CD25
R16397 T21986 T21988 compound CD4,CD25
R16398 T21987 T21988 compound +,CD25
R16399 T21988 T21991 npadvmod CD25,lysed
R16400 T21989 T21991 nsubjpass cells,lysed
R16401 T21990 T21991 auxpass were,lysed
R16402 T21991 T21991 ROOT lysed,lysed
R16403 T21992 T21991 cc and,lysed
R16404 T21993 T21991 conj loaded,lysed
R16405 T21994 T21993 advmod next,loaded
R16406 T21995 T21994 prep to,next
R16407 T21996 T21998 det a,ladder
R16408 T21997 T21998 amod protein-mass,ladder
R16409 T21998 T21995 pobj ladder,to
R16410 T21999 T21998 punct (,ladder
R16411 T22000 T21998 appos Magicmark,ladder
R16412 T22001 T22000 punct ",",Magicmark
R16413 T22002 T22000 appos Invitrogen,Magicmark
R16414 T22003 T22000 punct ),Magicmark
R16415 T22004 T21998 prep on,ladder
R16416 T22005 T22011 det a,gel
R16417 T22006 T22011 nmod NuPAGE,gel
R16418 T22007 T22006 nummod 4,NuPAGE
R16419 T22008 T22009 nummod 12,%
R16420 T22009 T22011 nmod %,gel
R16421 T22010 T22011 amod bis-tris,gel
R16422 T22011 T22004 pobj gel,on
R16423 T22012 T22013 punct (,Invitrogen
R16424 T22013 T22011 appos Invitrogen,gel
R16425 T22014 T21993 punct ),loaded
R16426 T22015 T21991 punct .,lysed
R16427 T22016 T22017 det The,proteins
R16428 T22017 T22019 nsubjpass proteins,electroblotted
R16429 T22018 T22019 auxpass were,electroblotted
R16430 T22019 T22019 ROOT electroblotted,electroblotted
R16431 T22020 T22019 prep onto,electroblotted
R16432 T22021 T22023 det a,membrane
R16433 T22022 T22023 compound PVDF,membrane
R16434 T22023 T22020 pobj membrane,onto
R16435 T22024 T22027 punct (,Science
R16436 T22025 T22027 compound Amersham,Science
R16437 T22026 T22027 compound Life,Science
R16438 T22027 T22023 appos Science,membrane
R16439 T22028 T22019 punct ),electroblotted
R16440 T22029 T22019 punct .,electroblotted
R16441 T22030 T22031 amod Unspecific,binding
R16442 T22031 T22033 nsubjpass binding,blocked
R16443 T22032 T22033 auxpass was,blocked
R16444 T22033 T22033 ROOT blocked,blocked
R16445 T22034 T22033 prep with,blocked
R16446 T22035 T22034 pobj BSA,with
R16447 T22036 T22033 punct ",",blocked
R16448 T22037 T22033 cc and,blocked
R16449 T22038 T22039 det the,membranes
R16450 T22039 T22042 nsubjpass membranes,incubated
R16451 T22040 T22042 auxpass were,incubated
R16452 T22041 T22042 advmod subsequently,incubated
R16453 T22042 T22033 conj incubated,blocked
R16454 T22043 T22042 prep with,incubated
R16455 T22044 T22046 det an,dilution
R16456 T22045 T22046 nummod 1:200,dilution
R16457 T22046 T22043 pobj dilution,with
R16458 T22047 T22046 prep of,dilution
R16459 T22048 T22049 compound goat,anti-FOXP3
R16460 T22049 T22047 pobj anti-FOXP3,of
R16461 T22050 T22046 prep in,dilution
R16462 T22051 T22050 pcomp blocking,in
R16463 T22052 T22051 dobj buffer,blocking
R16464 T22053 T22052 punct (,buffer
R16465 T22054 T22052 appos Abcam,buffer
R16466 T22055 T22052 punct ),buffer
R16467 T22056 T22051 npadvmod overnight,blocking
R16468 T22057 T22051 prep at,blocking
R16469 T22058 T22059 nummod 4,°
R16470 T22059 T22057 pobj °,at
R16471 T22060 T22050 pobj C,in
R16472 T22061 T22042 punct .,incubated
R16473 T22062 T22063 det The,blots
R16474 T22063 T22065 nsubjpass blots,developed
R16475 T22064 T22065 auxpass were,developed
R16476 T22065 T22065 ROOT developed,developed
R16477 T22066 T22065 advcl using,developed
R16478 T22067 T22070 det an,mAb
R16479 T22068 T22070 amod anti-goat,mAb
R16480 T22069 T22070 compound HRP-labeled,mAb
R16481 T22070 T22066 dobj mAb,using
R16482 T22071 T22073 punct (,Biosciences
R16483 T22072 T22073 compound Amersham,Biosciences
R16484 T22073 T22070 parataxis Biosciences,mAb
R16485 T22074 T22073 punct ),Biosciences
R16486 T22075 T22066 cc and,using
R16487 T22076 T22066 conj visualized,using
R16488 T22077 T22076 prep with,visualized
R16489 T22078 T22081 det a,camera
R16490 T22079 T22081 nmod LAS,camera
R16491 T22080 T22081 nummod 1000,camera
R16492 T22081 T22077 pobj camera,with
R16493 T22082 T22081 punct (,camera
R16494 T22083 T22081 appos Fuji,camera
R16495 T22084 T22081 punct ),camera
R16496 T22085 T22065 punct .,developed
R16497 T22086 T22088 nsubjpass Membranes,incubated
R16498 T22087 T22088 auxpass were,incubated
R16499 T22088 T22088 ROOT incubated,incubated
R16500 T22089 T22088 prep in,incubated
R16501 T22090 T22089 pcomp stripping,in
R16502 T22091 T22094 meta buffer,for
R16503 T22092 T22091 cc and,buffer
R16504 T22093 T22091 conj re-blocked,buffer
R16505 T22094 T22090 prep for,stripping
R16506 T22095 T22096 nummod 1,h
R16507 T22096 T22094 pobj h,for
R16508 T22097 T22088 punct .,incubated
R16509 T22098 T22099 det The,membranes
R16510 T22099 T22100 nsubj membranes,were
R16511 T22100 T22100 ROOT were,were
R16512 T22101 T22110 dep re-probed,Biotechnology
R16513 T22102 T22101 advcl using,re-probed
R16514 T22103 T22102 dobj anti-GATA3,using
R16515 T22104 T22103 punct (,anti-GATA3
R16516 T22105 T22103 appos HG3,anti-GATA3
R16517 T22106 T22103 nummod 31,anti-GATA3
R16518 T22107 T22110 punct ;,Biotechnology
R16519 T22108 T22109 compound Santa,Cruz
R16520 T22109 T22110 compound Cruz,Biotechnology
R16521 T22110 T22100 acomp Biotechnology,were
R16522 T22111 T22110 punct ),Biotechnology
R16523 T22112 T22110 punct ",",Biotechnology
R16524 T22113 T22110 conj anti-T-bet,Biotechnology
R16525 T22114 T22129 punct (,anti-phospho-SMAD2
R16526 T22115 T22129 nmod 4B10,anti-phospho-SMAD2
R16527 T22116 T22115 punct ",",4B10
R16528 T22117 T22118 compound Santa,Cruz
R16529 T22118 T22119 compound Cruz,Biotechnology
R16530 T22119 T22115 appos Biotechnology,4B10
R16531 T22120 T22115 punct ),4B10
R16532 T22121 T22115 punct ",",4B10
R16533 T22122 T22115 conj anti-GAPDH,4B10
R16534 T22123 T22122 punct (,anti-GAPDH
R16535 T22124 T22122 appos 6C5,anti-GAPDH
R16536 T22125 T22122 punct ",",anti-GAPDH
R16537 T22126 T22122 appos Ambion,anti-GAPDH
R16538 T22127 T22122 punct ),anti-GAPDH
R16539 T22128 T22122 punct ",",anti-GAPDH
R16540 T22129 T22113 appos anti-phospho-SMAD2,anti-T-bet
R16541 T22130 T22129 punct (,anti-phospho-SMAD2
R16542 T22131 T22129 appos 138D4,anti-phospho-SMAD2
R16543 T22132 T22129 punct ),anti-phospho-SMAD2
R16544 T22133 T22129 punct ",",anti-phospho-SMAD2
R16545 T22134 T22129 appos anti-phospho-STAT6,anti-phospho-SMAD2
R16546 T22135 T22136 punct (,5A4
R16547 T22136 T22134 appos 5A4,anti-phospho-STAT6
R16548 T22137 T22136 punct ),5A4
R16549 T22138 T22134 punct ",",anti-phospho-STAT6
R16550 T22139 T22134 cc and,anti-phospho-STAT6
R16551 T22140 T22134 conj anti-STAT6,anti-phospho-STAT6
R16552 T22141 T22144 punct (,Technology
R16553 T22142 T22144 compound Cell,Technology
R16554 T22143 T22144 compound Signaling,Technology
R16555 T22144 T22140 parataxis Technology,anti-STAT6
R16556 T22145 T22144 punct ),Technology
R16557 T22146 T22110 punct ",",Biotechnology
R16673 T22376 T22376 ROOT Administration,Administration
R16674 T22377 T22376 prep of,Administration
R16675 T22378 T22377 pobj cytokines,of
R16676 T22379 T22378 cc and,cytokines
R16677 T22380 T22378 conj antibodies,cytokines
R16678 T22381 T22378 prep in,cytokines
R16679 T22382 T22381 pobj vivo,in
R16680 T22383 T22376 punct .,Administration
R16681 T22384 T22391 nsubj Age,received
R16682 T22385 T22384 punct -,Age
R16683 T22386 T22384 cc and,Age
R16684 T22387 T22390 amod gender-matched,mice
R16685 T22388 T22390 amod normal,mice
R16686 T22389 T22390 compound B6,mice
R16687 T22390 T22384 conj mice,Age
R16688 T22391 T22391 ROOT received,received
R16689 T22392 T22394 det every,day
R16690 T22393 T22394 amod other,day
R16691 T22394 T22397 nmod day,ip
R16692 T22395 T22397 nmod intraperitoneal,ip
R16693 T22396 T22397 punct (,ip
R16694 T22397 T22391 dobj ip,received
R16695 T22398 T22397 punct ),ip
R16696 T22399 T22391 dobj injections,received
R16697 T22400 T22399 prep of,injections
R16698 T22401 T22400 pobj PBS,of
R16699 T22402 T22399 punct ",",injections
R16700 T22403 T22405 nummod 1.5,rmIL-4
R16701 T22404 T22405 compound μg,rmIL-4
R16702 T22405 T22399 appos rmIL-4,injections
R16703 T22406 T22405 punct ",",rmIL-4
R16704 T22407 T22408 nummod 50,μg
R16705 T22408 T22410 compound μg,mAb
R16706 T22409 T22410 compound anti-IL-4,mAb
R16707 T22410 T22405 appos mAb,rmIL-4
R16708 T22411 T22410 punct (,mAb
R16709 T22412 T22410 appos 11B11,mAb
R16710 T22413 T22412 cc or,11B11
R16711 T22414 T22412 conj MAB404,11B11
R16712 T22415 T22410 punct ),mAb
R16713 T22416 T22410 punct ",",mAb
R16714 T22417 T22405 cc or,rmIL-4
R16715 T22418 T22419 det a,mixture
R16716 T22419 T22405 conj mixture,rmIL-4
R16717 T22420 T22419 prep of,mixture
R16718 T22421 T22423 nummod 1.5,rmIL-4
R16719 T22422 T22423 compound μg,rmIL-4
R16720 T22423 T22420 pobj rmIL-4,of
R16721 T22424 T22419 cc plus,mixture
R16722 T22425 T22426 nummod 50,μg
R16723 T22426 T22428 compound μg,mAb
R16724 T22427 T22428 compound anti-IL-4,mAb
R16725 T22428 T22419 appos mAb,mixture
R16726 T22429 T22430 punct (,11B11
R16727 T22430 T22428 appos 11B11,mAb
R16728 T22431 T22430 cc or,11B11
R16729 T22432 T22430 conj MAB404,11B11
R16730 T22433 T22428 punct ),mAb
R16731 T22434 T22405 prep for,rmIL-4
R16732 T22435 T22436 nummod 7,d.
R16733 T22436 T22434 pobj d.,for
R16734 T22437 T22445 advmod Thereafter,analyzed
R16735 T22438 T22445 punct ",",analyzed
R16736 T22439 T22443 amod spleen,cells
R16737 T22440 T22439 cc and,spleen
R16738 T22441 T22439 conj lymph,spleen
R16739 T22442 T22443 compound node,cells
R16740 T22443 T22445 nsubjpass cells,analyzed
R16741 T22444 T22445 auxpass were,analyzed
R16742 T22445 T22391 advcl analyzed,received
R16743 T22446 T22445 agent by,analyzed
R16744 T22447 T22448 compound flow,cytometry
R16745 T22448 T22446 pobj cytometry,by
R16746 T22449 T22445 prep for,analyzed
R16747 T22450 T22449 pobj CD3,for
R16748 T22451 T22450 punct ",",CD3
R16749 T22452 T22450 conj CD4,CD3
R16750 T22453 T22445 punct ",",analyzed
R16751 T22454 T22445 cc and,analyzed
R16752 T22455 T22456 compound CD25,expression
R16753 T22456 T22445 conj expression,analyzed
R16754 T22457 T22391 punct .,received
R16755 T22458 T22460 det The,IL-4
R16756 T22459 T22460 amod anti-mouse,IL-4
R16757 T22460 T22464 nsubjpass IL-4,obtained
R16758 T22461 T22462 compound mAb,MAB404
R16759 T22462 T22460 appos MAB404,IL-4
R16760 T22463 T22464 auxpass was,obtained
R16761 T22464 T22464 ROOT obtained,obtained
R16762 T22465 T22464 prep from,obtained
R16763 T22466 T22467 compound R&D,Systems
R16764 T22467 T22465 pobj Systems,from
R16765 T22468 T22467 punct ",",Systems
R16766 T22469 T22472 det the,IL-4
R16767 T22470 T22472 amod second,IL-4
R16768 T22471 T22472 amod anti-mouse,IL-4
R16769 T22472 T22467 appos IL-4,Systems
R16770 T22473 T22474 compound mAb,11B11
R16771 T22474 T22476 nsubjpass 11B11,purchased
R16772 T22475 T22476 auxpass was,purchased
R16773 T22476 T22464 conj purchased,obtained
R16774 T22477 T22476 prep from,purchased
R16775 T22478 T22477 pobj eBioscience,from
R16776 T22479 T22464 punct .,obtained
R16968 T22754 T22754 ROOT ChIP,ChIP
R16969 T22755 T22754 punct .,ChIP
R16970 T22756 T22757 compound ChIP,analysis
R16971 T22757 T22759 nsubjpass analysis,performed
R16972 T22758 T22759 auxpass was,performed
R16973 T22759 T22759 ROOT performed,performed
R16974 T22760 T22759 prep according,performed
R16975 T22761 T22760 prep to,according
R16976 T22762 T22763 det the,manufacturer
R16977 T22763 T22765 poss manufacturer,protocol
R16978 T22764 T22763 case 's,manufacturer
R16979 T22765 T22761 pobj protocol,to
R16980 T22766 T22768 punct (,Biotechnology
R16981 T22767 T22768 compound Upstate,Biotechnology
R16982 T22768 T22765 appos Biotechnology,protocol
R16983 T22769 T22759 punct ),performed
R16984 T22770 T22759 prep with,performed
R16985 T22771 T22773 det the,modifications
R16986 T22772 T22773 amod following,modifications
R16987 T22773 T22770 pobj modifications,with
R16988 T22774 T22759 punct .,performed
R16989 T22775 T22778 nmod iTreg,cells
R16990 T22776 T22775 cc and,iTreg
R16991 T22777 T22775 conj Th2,iTreg
R16992 T22778 T22780 nsubjpass cells,fixed
R16993 T22779 T22780 auxpass were,fixed
R16994 T22780 T22780 ROOT fixed,fixed
R16995 T22781 T22780 prep with,fixed
R16996 T22782 T22783 nummod 1,%
R16997 T22783 T22784 compound %,formaldehyde
R16998 T22784 T22781 pobj formaldehyde,with
R16999 T22785 T22780 prep for,fixed
R17000 T22786 T22787 nummod 10,min
R17001 T22787 T22785 pobj min,for
R17002 T22788 T22787 prep at,min
R17003 T22789 T22790 compound room,temperature
R17004 T22790 T22788 pobj temperature,at
R17005 T22791 T22780 punct .,fixed
R17006 T22792 T22793 det The,chromatin
R17007 T22793 T22795 nsubjpass chromatin,sheared
R17008 T22794 T22795 auxpass was,sheared
R17009 T22795 T22795 ROOT sheared,sheared
R17010 T22796 T22795 prep to,sheared
R17011 T22797 T22798 nummod 200-1000,bp
R17012 T22798 T22796 pobj bp,to
R17013 T22799 T22798 prep of,bp
R17014 T22800 T22799 pobj length,of
R17015 T22801 T22795 agent by,sheared
R17016 T22802 T22801 pobj sonication,by
R17017 T22803 T22795 prep with,sheared
R17018 T22804 T22805 nummod five,pulses
R17019 T22805 T22803 pobj pulses,with
R17020 T22806 T22805 prep of,pulses
R17021 T22807 T22806 pobj 10,of
R17022 T22808 T22807 npadvmod s,10
R17023 T22809 T22805 prep at,pulses
R17024 T22810 T22811 nummod 30,%
R17025 T22811 T22812 compound %,power
R17026 T22812 T22809 pobj power,at
R17027 T22813 T22814 punct (,Bandelin
R17028 T22814 T22812 appos Bandelin,power
R17029 T22815 T22814 punct ),Bandelin
R17030 T22816 T22795 punct .,sheared
R17031 T22817 T22818 det The,chromatin
R17032 T22818 T22819 nsubj chromatin,was
R17033 T22819 T22851 ccomp was,used
R17034 T22820 T22819 acomp pre-cleared,was
R17035 T22821 T22820 prep for,pre-cleared
R17036 T22822 T22823 nummod 2,h
R17037 T22823 T22821 pobj h,for
R17038 T22824 T22823 prep with,h
R17039 T22825 T22826 amod normal,mouse
R17040 T22826 T22828 compound mouse,beads
R17041 T22827 T22828 compound IgG,beads
R17042 T22828 T22824 pobj beads,with
R17043 T22829 T22819 cc and,was
R17044 T22830 T22831 advmod then,incubated
R17045 T22831 T22819 conj incubated,was
R17046 T22832 T22831 prep with,incubated
R17047 T22833 T22834 amod anti-GATA3-agarose,beads
R17048 T22834 T22832 pobj beads,with
R17049 T22835 T22834 punct (,beads
R17050 T22836 T22834 appos HG3,beads
R17051 T22837 T22834 appos 31,beads
R17052 T22838 T22851 punct ;,used
R17053 T22839 T22840 compound Santa,Cruz
R17054 T22840 T22841 compound Cruz,Biotechnology
R17055 T22841 T22851 nsubjpass Biotechnology,used
R17056 T22842 T22841 punct ),Biotechnology
R17057 T22843 T22841 prep for,Biotechnology
R17058 T22844 T22845 nummod 2,h.
R17059 T22845 T22843 pobj h.,for
R17060 T22846 T22845 acl Washing,h.
R17061 T22847 T22846 cc and,Washing
R17062 T22848 T22849 compound elution,buffers
R17063 T22849 T22851 nsubjpass buffers,used
R17064 T22850 T22851 auxpass were,used
R17065 T22851 T22851 ROOT used,used
R17066 T22852 T22851 prep according,used
R17067 T22853 T22852 prep to,according
R17068 T22854 T22855 det the,protocol
R17069 T22855 T22853 pobj protocol,to
R17070 T22856 T22855 prep of,protocol
R17071 T22857 T22858 compound Upstate,Biotechnology
R17072 T22858 T22856 pobj Biotechnology,of
R17073 T22859 T22851 punct .,used
R17074 T22860 T22862 nsubjpass Crosslinks,reversed
R17075 T22861 T22862 auxpass were,reversed
R17076 T22862 T22862 ROOT reversed,reversed
R17077 T22863 T22862 agent by,reversed
R17078 T22864 T22863 pobj incubation,by
R17079 T22865 T22862 prep at,reversed
R17080 T22866 T22868 nummod 65,C
R17081 T22867 T22868 nummod °,C
R17082 T22868 T22865 pobj C,at
R17083 T22869 T22862 prep for,reversed
R17084 T22870 T22871 nummod 4,h
R17085 T22871 T22869 pobj h,for
R17086 T22872 T22862 prep in,reversed
R17087 T22873 T22874 det the,presence
R17088 T22874 T22872 pobj presence,in
R17089 T22875 T22874 prep of,presence
R17090 T22876 T22878 nummod 0.2,NaCl
R17091 T22877 T22878 compound M,NaCl
R17092 T22878 T22875 pobj NaCl,of
R17093 T22879 T22862 punct ",",reversed
R17094 T22880 T22862 cc and,reversed
R17095 T22881 T22882 det the,DNA
R17096 T22882 T22884 nsubjpass DNA,purified
R17097 T22883 T22884 auxpass was,purified
R17098 T22884 T22862 conj purified,reversed
R17099 T22885 T22884 agent by,purified
R17100 T22886 T22887 compound phenol/chloroform,extraction
R17101 T22887 T22885 pobj extraction,by
R17102 T22888 T22862 punct .,reversed
R17103 T22889 T22890 det The,amount
R17104 T22890 T22894 nsubjpass amount,determined
R17105 T22891 T22890 prep of,amount
R17106 T22892 T22891 pobj DNA,of
R17107 T22893 T22894 auxpass was,determined
R17108 T22894 T22894 ROOT determined,determined
R17109 T22895 T22894 agent by,determined
R17110 T22896 T22897 amod conventional,PCR
R17111 T22897 T22895 pobj PCR,by
R17112 T22898 T22894 punct .,determined
R17113 T22899 T22900 det The,PCR
R17114 T22900 T22901 nsubj PCR,addressed
R17115 T22901 T22901 ROOT addressed,addressed
R17116 T22902 T22901 prep for,addressed
R17117 T22903 T22906 det the,region
R17118 T22904 T22906 amod FOXP3,region
R17119 T22905 T22906 compound promoter,region
R17120 T22906 T22902 pobj region,for
R17121 T22907 T22901 dobj −,addressed
R17122 T22908 T22907 nummod 246,−
R17123 T22909 T22908 prep to,246
R17124 T22910 T22909 pobj −,to
R17125 T22911 T22910 appos 511,−
R17126 T22912 T22901 cc and,addressed
R17127 T22913 T22914 auxpass was,performed
R17128 T22914 T22901 conj performed,addressed
R17129 T22915 T22914 xcomp using,performed
R17130 T22916 T22918 det the,primers
R17131 T22917 T22918 amod following,primers
R17132 T22918 T22915 dobj primers,using
R17133 T22919 T22918 punct :,primers
R17134 T22920 T22925 nummod 5,′
R17135 T22921 T22923 punct ′,gtgccctttacgagt
R17136 T22922 T22923 punct -,gtgccctttacgagt
R17137 T22923 T22925 nmod gtgccctttacgagt,′
R17138 T22924 T22925 amod catctg-3,′
R17139 T22925 T22918 appos ′,primers
R17140 T22926 T22925 cc and,′
R17141 T22927 T22930 nummod 5,gtgccctttacgagtcatctg-3
R17142 T22928 T22930 punct ′,gtgccctttacgagtcatctg-3
R17143 T22929 T22930 punct -,gtgccctttacgagtcatctg-3
R17144 T22930 T22931 amod gtgccctttacgagtcatctg-3,′
R17145 T22931 T22925 conj ′,′
R17146 T22932 T22901 punct .,addressed
R17147 T22933 T22935 det The,products
R17148 T22934 T22935 compound PCR,products
R17149 T22935 T22937 nsubjpass products,visualized
R17150 T22936 T22937 auxpass were,visualized
R17151 T22937 T22937 ROOT visualized,visualized
R17152 T22938 T22937 advcl using,visualized
R17153 T22939 T22942 det an,gel
R17154 T22940 T22942 amod ethidium,gel
R17155 T22941 T22942 compound bromide,gel
R17156 T22942 T22938 dobj gel,using
R17157 T22943 T22937 punct .,visualized
R17371 T23192 T23193 amod Pull-down,assay
R17372 T23193 T23193 ROOT assay,assay
R17373 T23194 T23193 punct .,assay
R17374 T23195 T23198 nummod CD4,cells
R17375 T23196 T23198 nummod +,cells
R17376 T23197 T23198 compound T,cells
R17377 T23198 T23200 nsubjpass cells,stimulated
R17378 T23199 T23200 auxpass were,stimulated
R17379 T23200 T23200 ROOT stimulated,stimulated
R17380 T23201 T23200 prep with,stimulated
R17381 T23202 T23201 pobj PMA,with
R17382 T23203 T23202 cc and,PMA
R17383 T23204 T23202 conj ionomycin,PMA
R17384 T23205 T23200 prep for,stimulated
R17385 T23206 T23207 nummod 2,h
R17386 T23207 T23205 pobj h,for
R17387 T23208 T23200 prep at,stimulated
R17388 T23209 T23210 nummod 37,°
R17389 T23210 T23211 nummod °,C
R17390 T23211 T23208 pobj C,at
R17391 T23212 T23200 punct .,stimulated
R17392 T23213 T23214 det The,cells
R17393 T23214 T23216 nsubjpass cells,pelleted
R17394 T23215 T23216 auxpass were,pelleted
R17395 T23216 T23216 ROOT pelleted,pelleted
R17396 T23217 T23216 punct ",",pelleted
R17397 T23218 T23216 conj resuspended,pelleted
R17398 T23219 T23218 prep in,resuspended
R17399 T23220 T23221 compound buffer,C
R17400 T23221 T23219 pobj C,in
R17401 T23222 T23229 punct (,]
R17402 T23223 T23229 nummod 20,]
R17403 T23224 T23227 compound mM,pH
R17404 T23225 T23227 compound HEPES,pH
R17405 T23226 T23227 compound [,pH
R17406 T23227 T23229 compound pH,]
R17407 T23228 T23229 compound 7.9,]
R17408 T23229 T23221 appos ],C
R17409 T23230 T23229 punct ",",]
R17410 T23231 T23233 nummod 420,NaCl
R17411 T23232 T23233 compound mM,NaCl
R17412 T23233 T23229 appos NaCl,]
R17413 T23234 T23233 punct ",",NaCl
R17414 T23235 T23237 nummod 1.5,MgCl2
R17415 T23236 T23237 compound mM,MgCl2
R17416 T23237 T23233 appos MgCl2,NaCl
R17417 T23238 T23237 punct ",",MgCl2
R17418 T23239 T23241 nummod 0.2,EDTA
R17419 T23240 T23241 compound mM,EDTA
R17420 T23241 T23237 appos EDTA,MgCl2
R17421 T23242 T23241 punct ",",EDTA
R17422 T23243 T23245 nummod 1,DTT
R17423 T23244 T23245 compound mM,DTT
R17424 T23245 T23241 appos DTT,EDTA
R17425 T23246 T23245 punct ",",DTT
R17426 T23247 T23248 compound protease,inhibitors
R17427 T23248 T23259 nsubj inhibitors,lysed
R17428 T23249 T23251 compound [,]
R17429 T23250 T23251 compound Sigma,]
R17430 T23251 T23248 appos ],inhibitors
R17431 T23252 T23251 punct .,]
R17432 T23253 T23248 cc and,inhibitors
R17433 T23254 T23255 nummod 0.1,%
R17434 T23255 T23256 compound %,NP-40
R17435 T23256 T23248 conj NP-40,inhibitors
R17436 T23257 T23256 punct ),NP-40
R17437 T23258 T23256 cc and,NP-40
R17438 T23259 T23218 ccomp lysed,resuspended
R17439 T23260 T23259 prep on,lysed
R17440 T23261 T23260 pobj ice,on
R17441 T23262 T23259 prep for,lysed
R17442 T23263 T23264 nummod 15,min
R17443 T23264 T23262 pobj min,for
R17444 T23265 T23216 punct .,pelleted
R17445 T23266 T23267 amod Insoluble,material
R17446 T23267 T23269 nsubjpass material,removed
R17447 T23268 T23269 auxpass was,removed
R17448 T23269 T23269 ROOT removed,removed
R17449 T23270 T23269 agent by,removed
R17450 T23271 T23270 pobj centrifugation,by
R17451 T23272 T23269 punct .,removed
R17452 T23273 T23274 det The,supernatant
R17453 T23274 T23276 nsubjpass supernatant,diluted
R17454 T23275 T23276 auxpass was,diluted
R17455 T23276 T23276 ROOT diluted,diluted
R17456 T23277 T23276 oprd 1:3,diluted
R17457 T23278 T23277 prep with,1:3
R17458 T23279 T23280 compound buffer,D
R17459 T23280 T23278 pobj D,with
R17460 T23281 T23282 punct (,as
R17461 T23282 T23280 prep as,D
R17462 T23283 T23284 compound buffer,C
R17463 T23284 T23282 pobj C,as
R17464 T23285 T23276 punct ",",diluted
R17465 T23286 T23276 cc but,diluted
R17466 T23287 T23286 prep without,but
R17467 T23288 T23287 pobj NaCl,without
R17468 T23289 T23276 punct ),diluted
R17469 T23290 T23276 punct .,diluted
R17470 T23291 T23292 det The,lysates
R17471 T23292 T23294 nsubjpass lysates,incubated
R17472 T23293 T23294 auxpass were,incubated
R17473 T23294 T23294 ROOT incubated,incubated
R17474 T23295 T23294 prep with,incubated
R17475 T23296 T23297 nummod 10,μg
R17476 T23297 T23295 pobj μg,with
R17477 T23298 T23297 prep of,μg
R17478 T23299 T23298 pobj poly,of
R17479 T23300 T23301 punct (,dI-dC
R17480 T23301 T23299 appos dI-dC,poly
R17481 T23302 T23294 punct ),incubated
R17482 T23303 T23294 punct (,incubated
R17483 T23304 T23304 ROOT Sigma,Sigma
R17484 T23305 T23304 punct ),Sigma
R17485 T23306 T23294 cc and,incubated
R17486 T23307 T23308 nummod 70,μl
R17487 T23308 T23294 conj μl,incubated
R17488 T23309 T23308 prep of,μl
R17489 T23310 T23309 pobj streptavidin-agarose,of
R17490 T23311 T23313 punct (,Biosciences
R17491 T23312 T23313 compound Amersham,Biosciences
R17492 T23313 T23310 appos Biosciences,streptavidin-agarose
R17493 T23314 T23313 punct ),Biosciences
R17494 T23315 T23308 acl carrying,μl
R17495 T23316 T23317 amod biotinylated,oligonucleotides
R17496 T23317 T23315 dobj oligonucleotides,carrying
R17497 T23318 T23315 punct ",",carrying
R17498 T23319 T23308 prep for,μl
R17499 T23320 T23321 nummod 3,h
R17500 T23321 T23319 pobj h,for
R17501 T23322 T23308 prep at,μl
R17502 T23323 T23324 nummod 4,°
R17503 T23324 T23322 pobj °,at
R17504 T23325 T23308 npadvmod C,μl
R17505 T23326 T23294 punct .,incubated
R17506 T23327 T23328 det The,beads
R17507 T23328 T23330 nsubjpass beads,washed
R17508 T23329 T23330 auxpass were,washed
R17509 T23330 T23351 ccomp washed,heated
R17510 T23331 T23330 advmod twice,washed
R17511 T23332 T23330 prep with,washed
R17512 T23333 T23335 compound buffer,:D
R17513 T23334 T23335 compound C,:D
R17514 T23335 T23332 pobj :D,with
R17515 T23336 T23335 punct (,:D
R17516 T23337 T23335 appos 1:3,:D
R17517 T23338 T23335 punct ),:D
R17518 T23339 T23330 cc and,washed
R17519 T23340 T23330 conj resuspended,washed
R17520 T23341 T23340 prep in,resuspended
R17521 T23342 T23344 amod DTT-containing,buffer
R17522 T23343 T23344 compound loading,buffer
R17523 T23344 T23341 pobj buffer,in
R17524 T23345 T23346 punct (,NuPAGE
R17525 T23346 T23344 appos NuPAGE,buffer
R17526 T23347 T23351 punct ;,heated
R17527 T23348 T23351 nsubj Invitrogen,heated
R17528 T23349 T23348 punct ),Invitrogen
R17529 T23350 T23351 punct ",",heated
R17530 T23351 T23351 ROOT heated,heated
R17531 T23352 T23351 prep to,heated
R17532 T23353 T23354 compound 70,°
R17533 T23354 T23355 compound °,C
R17534 T23355 T23352 pobj C,to
R17535 T23356 T23351 prep for,heated
R17536 T23357 T23358 nummod 10,min
R17537 T23358 T23356 pobj min,for
R17538 T23359 T23351 punct ",",heated
R17539 T23360 T23351 cc and,heated
R17540 T23361 T23362 det the,eluants
R17541 T23362 T23351 conj eluants,heated
R17542 T23363 T23362 prep on,eluants
R17543 T23364 T23370 det a,gel
R17544 T23365 T23370 nmod NuPAGE,gel
R17545 T23366 T23365 nummod 4,NuPAGE
R17546 T23367 T23368 nummod 12,%
R17547 T23368 T23370 nmod %,gel
R17548 T23369 T23370 amod bis-tris,gel
R17549 T23370 T23363 pobj gel,on
R17550 T23371 T23372 punct (,Invitrogen
R17551 T23372 T23370 appos Invitrogen,gel
R17552 T23373 T23362 punct ),eluants
R17553 T23374 T23351 punct .,heated
R17554 T23375 T23376 det The,proteins
R17555 T23376 T23378 nsubjpass proteins,electroblotted
R17556 T23377 T23378 auxpass were,electroblotted
R17557 T23378 T23378 ROOT electroblotted,electroblotted
R17558 T23379 T23378 prep onto,electroblotted
R17559 T23380 T23382 det a,membrane
R17560 T23381 T23382 compound PVDF,membrane
R17561 T23382 T23379 pobj membrane,onto
R17562 T23383 T23385 punct (,Biosciences
R17563 T23384 T23385 compound Amersham,Biosciences
R17564 T23385 T23382 appos Biosciences,membrane
R17565 T23386 T23378 punct ),electroblotted
R17566 T23387 T23378 cc and,electroblotted
R17567 T23388 T23378 conj detected,electroblotted
R17568 T23389 T23388 xcomp using,detected
R17569 T23390 T23392 det an,mAb
R17570 T23391 T23392 compound anti-GATA3,mAb
R17571 T23392 T23389 dobj mAb,using
R17572 T23393 T23392 punct (,mAb
R17573 T23394 T23395 compound Santa,Cruz
R17574 T23395 T23396 compound Cruz,Biotechnology
R17575 T23396 T23392 appos Biotechnology,mAb
R17576 T23397 T23392 punct ),mAb
R17577 T23398 T23378 punct .,electroblotted
R17578 T23399 T23400 amod Accumulated,signals
R17579 T23400 T23402 nsubjpass signals,analyzed
R17580 T23401 T23402 auxpass were,analyzed
R17581 T23402 T23402 ROOT analyzed,analyzed
R17582 T23403 T23402 advcl using,analyzed
R17583 T23404 T23405 compound AIDA,software
R17584 T23405 T23403 dobj software,using
R17585 T23406 T23405 punct (,software
R17586 T23407 T23405 appos Raytest,software
R17587 T23408 T23405 punct ),software
R17588 T23409 T23402 punct .,analyzed
R13524 T17892 T17891 punct .,Mice
R13525 T17893 T17895 amod Normal,mice
R13526 T17894 T17895 compound B6,mice
R13527 T17895 T17897 nsubjpass mice,purchased
R13528 T17896 T17897 auxpass were,purchased
R13529 T17897 T17897 ROOT purchased,purchased
R13530 T17898 T17897 prep from,purchased
R13531 T17899 T17901 det the,Laboratories
R13532 T17900 T17901 compound Jackson,Laboratories
R13533 T17901 T17898 pobj Laboratories,from
R13534 T17902 T17901 punct (,Laboratories
R13535 T17903 T17904 compound Bar,Harbor
R13536 T17904 T17901 appos Harbor,Laboratories
R13537 T17905 T17904 punct ",",Harbor
R13538 T17906 T17904 npadvmod Maine,Harbor
R13539 T17907 T17901 punct ),Laboratories
R13540 T17908 T17897 punct .,purchased
R13541 T17909 T17910 compound Transgenic,DO11
R13542 T17910 T17914 nsubj DO11,expressing
R13543 T17911 T17912 nummod .10,mice
R13544 T17912 T17910 appos mice,DO11
R13545 T17913 T17910 punct ",",DO11
R13546 T17914 T17930 advcl expressing,backcrossed
R13547 T17915 T17918 det a,receptor
R13548 T17916 T17918 compound T,receptor
R13549 T17917 T17918 compound cell,receptor
R13550 T17918 T17914 dobj receptor,expressing
R13551 T17919 T17918 prep for,receptor
R13552 T17920 T17919 pobj OVA323,for
R13553 T17921 T17922 nummod 339,peptide
R13554 T17922 T17918 appos peptide,receptor
R13555 T17923 T17922 prep in,peptide
R13556 T17924 T17925 det the,context
R13557 T17925 T17923 pobj context,in
R13558 T17926 T17925 prep of,context
R13559 T17927 T17926 pobj H-2d,of
R13560 T17928 T17930 punct ",",backcrossed
R13561 T17929 T17930 auxpass were,backcrossed
R13562 T17930 T17930 ROOT backcrossed,backcrossed
R13563 T17931 T17930 prep with,backcrossed
R13564 T17932 T17931 pobj mice,with
R13565 T17933 T17932 acl expressing,mice
R13566 T17934 T17947 nmod GATA-3,[
R13567 T17935 T17934 punct ",",GATA-3
R13568 T17936 T17934 acl driven,GATA-3
R13569 T17937 T17936 agent by,driven
R13570 T17938 T17943 det the,region
R13571 T17939 T17943 amod human,region
R13572 T17940 T17943 compound CD2,region
R13573 T17941 T17942 compound locus,control
R13574 T17942 T17943 compound control,region
R13575 T17943 T17937 pobj region,by
R13576 T17944 T17945 punct (,CD2-GATA3
R13577 T17945 T17943 appos CD2-GATA3,region
R13578 T17946 T17947 punct ),[
R13579 T17947 T17949 nmod [,]
R13580 T17948 T17949 nummod 22,]
R13581 T17949 T17933 dobj ],expressing
R13582 T17950 T17930 punct ",",backcrossed
R13583 T17951 T17930 advcl resulting,backcrossed
R13584 T17952 T17951 prep in,resulting
R13585 T17953 T17954 amod DO11.10xCD2-GATA3,mice
R13586 T17954 T17952 pobj mice,in
R13587 T17955 T17930 punct .,backcrossed
R13588 T17956 T17961 nsubjpass Mice,backcrossed
R13589 T17957 T17956 acl used,Mice
R13590 T17958 T17957 prep for,used
R13591 T17959 T17958 pobj experiments,for
R13592 T17960 T17961 auxpass were,backcrossed
R13593 T17961 T17961 ROOT backcrossed,backcrossed
R13594 T17962 T17961 prep on,backcrossed
R13595 T17963 T17965 det a,background
R13596 T17964 T17965 compound BALB/c,background
R13597 T17965 T17962 pobj background,on

UBERON-AE

Id Subject Object Predicate Lexical cue
T18065 693-698 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T18776 2737-2742 http://purl.obolibrary.org/obo/UBERON_0002391 denotes lymph
T18777 2737-2748 http://purl.obolibrary.org/obo/UBERON_0000029 denotes lymph nodes
T18778 2753-2760 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleens
T18779 2846-2857 http://purl.obolibrary.org/obo/UBERON_0002371 denotes bone marrow
T19753 4250-4255 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T19754 4568-4573 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T22241 9309-9315 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleen
T22242 9320-9325 http://purl.obolibrary.org/obo/UBERON_0002391 denotes lymph
T22243 9320-9330 http://purl.obolibrary.org/obo/UBERON_0000029 denotes lymph node

GO-BP

Id Subject Object Predicate Lexical cue
T17777 473-476 http://purl.obolibrary.org/obo/GO_0007568 denotes age
T18078 936-939 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T18079 1034-1048 http://purl.obolibrary.org/obo/GO_0072602 denotes IL-4 secretion
T18080 1039-1048 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T18337 1273-1276 http://purl.obolibrary.org/obo/GO_0033968 denotes GCA
T18338 1492-1495 http://purl.obolibrary.org/obo/GO_0033968 denotes GCA
T18339 1277-1280 http://purl.obolibrary.org/obo/GO_0004096 denotes CAT
T18340 2056-2059 http://purl.obolibrary.org/obo/GO_0004096 denotes CAT
T18341 1945-1948 http://purl.obolibrary.org/obo/GO_0004069 denotes AAT
T18342 2008-2011 http://purl.obolibrary.org/obo/GO_0047801 denotes CGT
T18791 3101-3116 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T18792 3133-3135 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T19112 3153-3173 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T19520 4126-4129 http://purl.obolibrary.org/obo/GO_0004096 denotes CAT
T19521 4182-4184 http://purl.obolibrary.org/obo/GO_0033968 denotes GA
T19765 4631-4650 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T19766 4631-4650 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T19767 4631-4650 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T19768 4631-4650 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T19769 4631-4650 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity
T20045 4848-4857 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T20046 4848-4862 http://purl.obolibrary.org/obo/GO_0032774 denotes synthesis. RNA
T20047 4953-4974 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse transcription
T20048 5018-5039 http://purl.obolibrary.org/obo/GO_0001171 denotes reverse-transcription
T20049 4961-4974 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T20050 5026-5039 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T20444 5609-5621 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T22259 9057-9060 http://purl.obolibrary.org/obo/GO_0007568 denotes Age
T22974 11501-11508 http://purl.obolibrary.org/obo/GO_0023052 denotes signals

GO-MF

Id Subject Object Predicate Lexical cue
T18256 1034-1038 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T18701 1273-1276 http://purl.obolibrary.org/obo/GO_0033968 denotes GCA
T18702 1492-1495 http://purl.obolibrary.org/obo/GO_0033968 denotes GCA
T18703 1277-1280 http://purl.obolibrary.org/obo/GO_0004096 denotes CAT
T18704 2056-2059 http://purl.obolibrary.org/obo/GO_0004096 denotes CAT
T18705 1829-1838 http://purl.obolibrary.org/obo/GO_0031386 denotes Ubiquitin
T18706 2420-2429 http://purl.obolibrary.org/obo/GO_0031386 denotes ubiquitin
T18707 1945-1948 http://purl.obolibrary.org/obo/GO_0004069 denotes AAT
T18708 2008-2011 http://purl.obolibrary.org/obo/GO_0047801 denotes CGT
T19010 3133-3135 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T19403 3414-3419 http://purl.obolibrary.org/obo/GO_0005143 denotes IL-12
T19404 3521-3526 http://purl.obolibrary.org/obo/GO_0005143 denotes IL-12
T19405 3614-3619 http://purl.obolibrary.org/obo/GO_0005143 denotes IL-12
T19406 3434-3438 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T19407 3482-3486 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T19408 3634-3638 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T19409 3695-3699 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T19711 4126-4129 http://purl.obolibrary.org/obo/GO_0004096 denotes CAT
T19712 4182-4184 http://purl.obolibrary.org/obo/GO_0033968 denotes GA
T20001 4631-4650 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T20002 4631-4650 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T20003 4631-4650 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T20004 4631-4650 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T20005 4631-4650 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity
T20878 6168-6172 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T20879 6276-6286 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T21593 6550-6558 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T21594 6675-6683 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T21595 7381-7389 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T21596 7456-7464 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T21597 8008-8018 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T21598 7465-7472 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T22157 8391-8398 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T22491 9037-9047 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T22492 9187-9191 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T22493 9261-9265 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T22494 9419-9423 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T22495 9488-9492 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4

GO-CC

Id Subject Object Predicate Lexical cue
T18009 150-154 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T18257 654-659 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18258 668-673 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18259 838-843 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T18260 907-911 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T18261 936-939 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T18709 1281-1284 http://purl.obolibrary.org/obo/GO_0005579 denotes TCC
T18710 1983-1986 http://purl.obolibrary.org/obo/GO_0030896 denotes CCC
T19011 2679-2684 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19012 2808-2813 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19013 2876-2881 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19014 3021-3026 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19015 3059-3072 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T19410 3153-3157 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T19411 3280-3285 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19412 3654-3659 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T19413 3356-3360 http://purl.obolibrary.org/obo/GO_0071735 denotes IgG1
T19713 4134-4137 http://purl.obolibrary.org/obo/GO_0030896 denotes CCC
T20006 4229-4234 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T20007 4409-4414 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T20638 5635-5640 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T20639 5787-5792 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T20640 5981-5994 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T20880 6006-6019 http://purl.obolibrary.org/obo/GO_0005622 denotes Intracellular
T20881 6041-6046 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T20882 6276-6286 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T20883 6276-6286 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T21599 6493-6497 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T21600 6809-6813 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T21601 7937-7941 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T21602 8030-8034 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T21603 6550-6558 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T21604 6675-6683 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T21605 7381-7389 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T21606 7456-7464 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T21607 8008-8018 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T21608 6550-6558 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T21609 6675-6683 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T21610 7381-7389 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T21611 7456-7464 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T21612 8008-8018 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T21613 6809-6821 http://purl.obolibrary.org/obo/GO_0009986 denotes cell surface
T21614 7183-7187 http://purl.obolibrary.org/obo/GO_0071735 denotes IgG1
T21615 7195-7200 http://purl.obolibrary.org/obo/GO_0071735 denotes IgG2a
T21616 7628-7631 http://purl.obolibrary.org/obo/GO_0005680 denotes APC
T21617 7906-7909 http://purl.obolibrary.org/obo/GO_0005680 denotes APC
T21618 7801-7816 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellularly
T22158 8174-8179 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T22159 8346-8354 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T22160 8429-8438 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T22161 8757-8766 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T22496 9037-9047 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T22497 9037-9047 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T22498 9331-9336 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T22946 9683-9688 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T22947 9757-9766 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T22948 9872-9881 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T23412 10575-10580 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T23413 10641-10646 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T23414 11392-11400 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane

sentences

Id Subject Object Predicate Lexical cue
T17772 23-28 Sentence denotes Mice.
T17773 29-109 Sentence denotes Normal B6 mice were purchased from the Jackson Laboratories (Bar Harbor, Maine).
T17774 110-356 Sentence denotes Transgenic DO11.10 mice, expressing a T cell receptor for OVA323–339 peptide in the context of H-2d, were backcrossed with mice expressing GATA-3, driven by the human CD2 locus control region (CD2-GATA3) [22], resulting in DO11.10xCD2-GATA3 mice.
T17775 357-488 Sentence denotes Mice used for experiments were backcrossed on a BALB/c background for a minimum of eight generations and used at an age of 8–12 wk.
T17776 489-632 Sentence denotes Mice were housed under specific pathogen-free conditions and all animal studies were performed according to institutional and state guidelines.
T18073 634-660 Sentence denotes Isolation of CD4+ T cells.
T18074 661-816 Sentence denotes CD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60].
T18075 817-887 Sentence denotes The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%.
T18076 888-995 Sentence denotes Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive.
T18077 996-1049 Sentence denotes The clones were characterized by high IL-4 secretion.
T18328 1051-1078 Sentence denotes Quantitative real-time PCR.
T18329 1079-1507 Sentence denotes The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′.
T18330 1508-1697 Sentence denotes The prepared cDNAs were amplified using SYBR-PCR mastermix (Biorad) according to the recommendations of the manufacturer in an ABI PRISM 7000 Sequence Detection System (Applied Biosystems).
T18331 1698-1828 Sentence denotes Quantitative PCR of murine samples was performed with Brilliant SYBR Green QPCR master mix (Stratagene) and the following primers:
T18332 1829-2075 Sentence denotes Ubiquitin C, 5′- AGG TCA AAC AGG AAG ACA GAC GTA-3′ and 5′-TCACACCCAAGAACAAGCACA-3′; Smad-7, 5′-GAA ACC GGG GGA ACG AAT TAT-3′ and 5′-CGC GAG TCT TCT CCT CCC A-3′; TGF-ß1, 5′-TGA CGT CAC TGG AGT TGT ACG G-3′ and 5′-GGT TCA TGT CAT GGA TGG TGC-3′.
T18333 2076-2214 Sentence denotes Primer pairs were evaluated for integrity by analysis of the amplification plot, dissociation curves, and efficiency of PCR amplification.
T18334 2215-2367 Sentence denotes PCR conditions were 10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C and 60 °C for 1 min using an 7300 real-time PCR system (Applied Biosystems).
T18335 2368-2517 Sentence denotes PCR amplification of the housekeeping gene encoding ubiquitin C was performed during each run for each sample to allow normalization between samples.
T18336 2518-2633 Sentence denotes Relative quantification and calculation of the range of confidence was performed using the comparative ΔΔCT method.
T18787 2635-2665 Sentence denotes Inducible murine Treg culture.
T18788 2666-2797 Sentence denotes Naive CD4+ T cells (CD4+, CD62L+, and CD25–) were isolated from pooled lymph nodes and spleens by FACS (FACS Aria, BD Biosciences).
T18789 2798-3009 Sentence denotes 5 × 105 T cells were co-cultured with 2.5 × 104 bone marrow–derived dendritic cells [62] and 0.01 μg/ml OVA323–339 peptide (Ansynth) in the presence or absence of 20-ng/μl rhTGF-ß1 (Peprotech) in 48-well plates.
T18790 3010-3140 Sentence denotes After 4 d, cells were harvested and analyzed for intracellular FOXP3 expression by FACS or gene expression by quantitative RT-PCR.
T19106 3142-3174 Sentence denotes In vitro T cell differentiation.
T19107 3175-3404 Sentence denotes CD4+ CD45RA+ magnetically-sorted (CD45RO depletion, MACS, according to the protocol of the manufacturer) cells were stimulated with immobilized plate-bound anti-CD3 (1 μg/ml, Okt3, IgG1) and anti-CD28 (2 μg/ml) in Th1 conditions:
T19108 3405-3472 Sentence denotes 25 ng/ml IL-12, 5 μg/ml anti-IL-4 (R&D systems); in Th2 conditions:
T19109 3473-3564 Sentence denotes 25 ng/ml IL-4, 5 μg/ml anti-IFN-γ, 5 μg/ml anti-IL-12 (R&D systems); or in Treg conditions:
T19110 3565-3639 Sentence denotes 10 ng/ml TGF-β, 5 μg/ml anti-IFN-γ, 5 μg/ml anti-IL-12, 5 μg/ml anti-IL-4.
T19111 3640-3711 Sentence denotes Proliferating cells were expanded in medium containing IL-2 (30 ng/ml).
T19516 3713-3781 Sentence denotes Cloning of the FOXP3 promoter and construction of mutant constructs.
T19517 3782-3899 Sentence denotes The FOXP3 promoter was cloned into the pGL3 basic vector (Promega Biotech) to generate the pGL3 FOXP3 −511/+176 [24].
T19518 3900-4056 Sentence denotes Site-directed mutagenesis in the FOXP3 promoter region were introduced using the QuickChange kit (Stratagene), according to the manufacturer's instructions.
T19519 4057-4185 Sentence denotes The following primer and its complementary strand were used: GTT TCT CAT GAG CCC TAT TAA GTC ATT CTT ACC TCT CAC CTC TGT GGT GA.
T19761 4187-4226 Sentence denotes Transfections and reporter gene assays.
T19762 4227-4304 Sentence denotes T cells were rested in serum-free AIM-V medium (Life Technologies) overnight.
T19763 4305-4763 Sentence denotes 3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions.
T19764 4764-4823 Sentence denotes Data were normalized by the activity of renilla luciferase.
T20042 4825-4858 Sentence denotes RNA isolation and cDNA synthesis.
T20043 4859-4952 Sentence denotes RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol.
T20044 4953-5132 Sentence denotes Reverse transcription of human samples was performed with TaqMan reverse-transcription reagents (Applied Biosystems) with random hexamers according to the manufacturer's protocol.
T20171 5134-5159 Sentence denotes Recombinant TAT proteins.
T20172 5160-5351 Sentence denotes The cDNAs encoding GATA3 protein or the truncated GATA3 (lacking the two zinc fingers) were cloned in frame into an expression vector along with the TAT sequence as previously described [63].
T20173 5352-5477 Sentence denotes Proteins were expressed in BL21 Star (DE3)pLysS (Invitrogen) and lysates were purified by Ni2+-chelate column chromatography.
T20174 5478-5597 Sentence denotes Both TAT-linked proteins were more than 95% pure, based on Coomassie blue staining of sodium disulfate acrylamide gels.
T20439 5599-5622 Sentence denotes TAT-GATA3 transduction.
T20440 5623-5771 Sentence denotes CD4+CD45RA+ cells were cultured in AIMV medium and transduced with 20 nM, 10 nM, or 500 nM of full-length or truncated GATA3 over the course of 4 h.
T20441 5772-5876 Sentence denotes After 4 h, the cells were washed and activated with soluble anti-CD3 and anti-CD28 and TGF-β (10 ng/ml).
T20442 5877-5937 Sentence denotes Each day, the TAT proteins were freshly added to the medium.
T20443 5938-6004 Sentence denotes FOXP3 expression was measured after 5 d by intracellular staining.
T20710 6006-6038 Sentence denotes Intracellular cytokine staining.
T20711 6039-6134 Sentence denotes T cells were stimulated with 2 × 10−7 M PMA and 1 μg/ml of ionomycin (Sigma Chemicals) for 4 h.
T20712 6135-6188 Sentence denotes The following mAb was used: anti-IL-4-PE (8D4–8, BD).
T20713 6189-6287 Sentence denotes Matched isotype controls were used at the same protein concentration as the respective antibodies.
T20714 6288-6428 Sentence denotes Four-color FACS was performed using an EPICS XL-MCL (Beckman Coulter) using the software Expo32 version for data acquisition and evaluation.
T20958 6430-6445 Sentence denotes Flow cytometry.
T20959 6446-6804 Sentence denotes For analysis of FOXP3 expression at the single-cell level, cells were first stained with the monoclonal antibody CD25 (Beckman Coulter), and after fixation and permeabilization, cells were incubated with PE-conjugated monoclonal antibody PCH101 (anti-human FOXP3; eBioscience) based on the manufacturer's recommendations and subjected to FACS (EPICS XL-MCL).
T20960 6805-7130 Sentence denotes For cell surface marker staining, cells were incubated for 20 min at 4°C in staining buffer with the following antibodies: anti–CD152-PE (CTLA-4; BD), anti–PD-1 (eBiosciences), anti-GITR (R & D Systems), anti-CD69 (Beckman Coulter), anti-CD103 (DakoCytomation), anti-CD62L (Beckman Coulter), or anti-HLA-DR (Beckman Coulter).
T20961 7131-7201 Sentence denotes The controls were FITC, PE, or ECD-conjugated mouse IgG1 or rat IgG2a.
T20962 7202-7364 Sentence denotes For staining of mouse cells, the following mAbs from BD Biosciences were used following standard techniques as described above: anti-CD3, anti-CD4, and anti-CD25.
T20963 7365-7473 Sentence denotes Anti-FcγRII/III antibody (2.4G2, ATCC) was included in all stainings to reduce nonspecific antibody binding.
T20964 7474-7649 Sentence denotes To isolate naive murine CD4 T cells from murine DO11.10 or DO11.10xCD2-GATA3 T cells, cells were stained with anti-CD25-FITC, anti-CD62L-PE, and anti-CD4-APC prior to sorting.
T20965 7650-7718 Sentence denotes Dead cells were excluded with 4′',6-Diamidino-2-phenylindole (DAPI).
T20966 7719-7992 Sentence denotes To analyze murine Foxp3 expression in inducible Treg cultures, cells were stained intracellularly with anti-Foxp3-PE according to manufacturer's instruction, in conjunction with anti-CD4-APC and LIVE/DEAD fixable dead cell stain kit (Invitrogen) to discriminate live cells.
T20967 7993-8095 Sentence denotes All monoclonal antibodies for murine cell stainings were purchased from eBioscience or BD Biosciences.
T21784 8097-8114 Sentence denotes Western blotting.
T21785 8115-8300 Sentence denotes For FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen).
T21786 8301-8379 Sentence denotes The proteins were electroblotted onto a PVDF membrane (Amersham Life Science).
T21787 8380-8683 Sentence denotes Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji).
T21788 8684-8752 Sentence denotes Membranes were incubated in stripping buffer and re-blocked for 1 h.
T21789 8753-9003 Sentence denotes The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),
T22255 9005-9056 Sentence denotes Administration of cytokines and antibodies in vivo.
T22256 9057-9296 Sentence denotes Age- and gender-matched normal B6 mice received every other day intraperitoneal (ip) injections of PBS, 1.5 μg rmIL-4, 50 μg anti-IL-4 mAb (11B11 or MAB404), or a mixture of 1.5 μg rmIL-4 plus 50 μg anti-IL-4 mAb (11B11 or MAB404) for 7 d.
T22257 9297-9403 Sentence denotes Thereafter, spleen and lymph node cells were analyzed by flow cytometry for CD3, CD4, and CD25 expression.
T22258 9404-9534 Sentence denotes The anti-mouse IL-4 mAb MAB404 was obtained from R&D Systems, the second anti-mouse IL-4 mAb 11B11 was purchased from eBioscience.
T22565 9536-9541 Sentence denotes ChIP.
T22566 9542-9752 Sentence denotes ChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature.
T22567 9753-9867 Sentence denotes The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin).
T22568 9868-10026 Sentence denotes The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h.
T22569 10027-10261 Sentence denotes Washing and elution buffers were used according to the protocol of Upstate Biotechnology. Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction.
T22570 10262-10315 Sentence denotes The amount of DNA was determined by conventional PCR.
T22571 10316-10423 Sentence denotes The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers:
T22572 10424-10485 Sentence denotes 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′.
T22573 10486-10549 Sentence denotes The PCR products were visualized using an ethidium bromide gel.
T22967 10551-10567 Sentence denotes Pull-down assay.
T22968 10568-10832 Sentence denotes CD4+ T cells were stimulated with PMA and ionomycin for 2 h at 37°C. The cells were pelleted, resuspended in buffer C (20 mM HEPES [pH 7.9], 420 mM NaCl, 1.5 mM MgCl2, 0.2 mM EDTA, 1 mM DTT, protease inhibitors [Sigma]. and 0.1% NP-40) and lysed on ice for 15 min.
T22969 10833-10882 Sentence denotes Insoluble material was removed by centrifugation.
T22970 10883-10961 Sentence denotes The supernatant was diluted 1:3 with buffer D (as buffer C, but without NaCl).
T22971 10962-11346 Sentence denotes The lysates were incubated with 10 μg of poly(dI-dC) (Sigma) and 70 μl of streptavidin-agarose (Amersham Biosciences) carrying biotinylated oligonucleotides, for 3 h at 4 °C. The beads were washed twice with buffer C:D (1:3) and resuspended in DTT-containing loading buffer (NuPAGE; Invitrogen), heated to 70 °C for 10 min, and the eluants on a NuPAGE 4–12% bis-tris gel (Invitrogen).
T22972 11347-11488 Sentence denotes The proteins were electroblotted onto a PVDF membrane (Amersham Biosciences) and detected using an anti-GATA3 mAb (Santa Cruz Biotechnology).
T22973 11489-11553 Sentence denotes Accumulated signals were analyzed using AIDA software (Raytest).
T257 0-21 Sentence denotes Materials and Methods
T258 23-28 Sentence denotes Mice.
T259 29-109 Sentence denotes Normal B6 mice were purchased from the Jackson Laboratories (Bar Harbor, Maine).
T260 110-356 Sentence denotes Transgenic DO11.10 mice, expressing a T cell receptor for OVA323–339 peptide in the context of H-2d, were backcrossed with mice expressing GATA-3, driven by the human CD2 locus control region (CD2-GATA3) [22], resulting in DO11.10xCD2-GATA3 mice.
T261 357-488 Sentence denotes Mice used for experiments were backcrossed on a BALB/c background for a minimum of eight generations and used at an age of 8–12 wk.
T262 489-632 Sentence denotes Mice were housed under specific pathogen-free conditions and all animal studies were performed according to institutional and state guidelines.
T263 634-660 Sentence denotes Isolation of CD4+ T cells.
T264 661-816 Sentence denotes CD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60].
T265 817-887 Sentence denotes The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%.
T266 888-995 Sentence denotes Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive.
T267 996-1049 Sentence denotes The clones were characterized by high IL-4 secretion.
T268 1051-1078 Sentence denotes Quantitative real-time PCR.
T269 1079-1507 Sentence denotes The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′.
T270 1508-1697 Sentence denotes The prepared cDNAs were amplified using SYBR-PCR mastermix (Biorad) according to the recommendations of the manufacturer in an ABI PRISM 7000 Sequence Detection System (Applied Biosystems).
T271 1698-1828 Sentence denotes Quantitative PCR of murine samples was performed with Brilliant SYBR Green QPCR master mix (Stratagene) and the following primers:
T272 1829-2075 Sentence denotes Ubiquitin C, 5′- AGG TCA AAC AGG AAG ACA GAC GTA-3′ and 5′-TCACACCCAAGAACAAGCACA-3′; Smad-7, 5′-GAA ACC GGG GGA ACG AAT TAT-3′ and 5′-CGC GAG TCT TCT CCT CCC A-3′; TGF-ß1, 5′-TGA CGT CAC TGG AGT TGT ACG G-3′ and 5′-GGT TCA TGT CAT GGA TGG TGC-3′.
T273 2076-2214 Sentence denotes Primer pairs were evaluated for integrity by analysis of the amplification plot, dissociation curves, and efficiency of PCR amplification.
T274 2215-2367 Sentence denotes PCR conditions were 10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C and 60 °C for 1 min using an 7300 real-time PCR system (Applied Biosystems).
T275 2368-2517 Sentence denotes PCR amplification of the housekeeping gene encoding ubiquitin C was performed during each run for each sample to allow normalization between samples.
T276 2518-2633 Sentence denotes Relative quantification and calculation of the range of confidence was performed using the comparative ΔΔCT method.
T277 2635-2665 Sentence denotes Inducible murine Treg culture.
T278 2666-2797 Sentence denotes Naive CD4+ T cells (CD4+, CD62L+, and CD25–) were isolated from pooled lymph nodes and spleens by FACS (FACS Aria, BD Biosciences).
T279 2798-3009 Sentence denotes 5 × 105 T cells were co-cultured with 2.5 × 104 bone marrow–derived dendritic cells [62] and 0.01 μg/ml OVA323–339 peptide (Ansynth) in the presence or absence of 20-ng/μl rhTGF-ß1 (Peprotech) in 48-well plates.
T280 3010-3140 Sentence denotes After 4 d, cells were harvested and analyzed for intracellular FOXP3 expression by FACS or gene expression by quantitative RT-PCR.
T281 3142-3174 Sentence denotes In vitro T cell differentiation.
T282 3175-3404 Sentence denotes CD4+ CD45RA+ magnetically-sorted (CD45RO depletion, MACS, according to the protocol of the manufacturer) cells were stimulated with immobilized plate-bound anti-CD3 (1 μg/ml, Okt3, IgG1) and anti-CD28 (2 μg/ml) in Th1 conditions:
T283 3405-3472 Sentence denotes 25 ng/ml IL-12, 5 μg/ml anti-IL-4 (R&D systems); in Th2 conditions:
T284 3473-3564 Sentence denotes 25 ng/ml IL-4, 5 μg/ml anti-IFN-γ, 5 μg/ml anti-IL-12 (R&D systems); or in Treg conditions:
T285 3565-3639 Sentence denotes 10 ng/ml TGF-β, 5 μg/ml anti-IFN-γ, 5 μg/ml anti-IL-12, 5 μg/ml anti-IL-4.
T286 3640-3711 Sentence denotes Proliferating cells were expanded in medium containing IL-2 (30 ng/ml).
T287 3713-3781 Sentence denotes Cloning of the FOXP3 promoter and construction of mutant constructs.
T288 3782-3899 Sentence denotes The FOXP3 promoter was cloned into the pGL3 basic vector (Promega Biotech) to generate the pGL3 FOXP3 −511/+176 [24].
T289 3900-4056 Sentence denotes Site-directed mutagenesis in the FOXP3 promoter region were introduced using the QuickChange kit (Stratagene), according to the manufacturer's instructions.
T290 4057-4185 Sentence denotes The following primer and its complementary strand were used: GTT TCT CAT GAG CCC TAT TAA GTC ATT CTT ACC TCT CAC CTC TGT GGT GA.
T291 4187-4226 Sentence denotes Transfections and reporter gene assays.
T292 4227-4304 Sentence denotes T cells were rested in serum-free AIM-V medium (Life Technologies) overnight.
T293 4305-4543 Sentence denotes 3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector.
T294 4544-4763 Sentence denotes After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions.
T295 4764-4823 Sentence denotes Data were normalized by the activity of renilla luciferase.
T296 4825-4858 Sentence denotes RNA isolation and cDNA synthesis.
T297 4859-4952 Sentence denotes RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol.
T298 4953-5132 Sentence denotes Reverse transcription of human samples was performed with TaqMan reverse-transcription reagents (Applied Biosystems) with random hexamers according to the manufacturer's protocol.
T299 5134-5159 Sentence denotes Recombinant TAT proteins.
T300 5160-5351 Sentence denotes The cDNAs encoding GATA3 protein or the truncated GATA3 (lacking the two zinc fingers) were cloned in frame into an expression vector along with the TAT sequence as previously described [63].
T301 5352-5477 Sentence denotes Proteins were expressed in BL21 Star (DE3)pLysS (Invitrogen) and lysates were purified by Ni2+-chelate column chromatography.
T302 5478-5597 Sentence denotes Both TAT-linked proteins were more than 95% pure, based on Coomassie blue staining of sodium disulfate acrylamide gels.
T303 5599-5622 Sentence denotes TAT-GATA3 transduction.
T304 5623-5771 Sentence denotes CD4+CD45RA+ cells were cultured in AIMV medium and transduced with 20 nM, 10 nM, or 500 nM of full-length or truncated GATA3 over the course of 4 h.
T305 5772-5876 Sentence denotes After 4 h, the cells were washed and activated with soluble anti-CD3 and anti-CD28 and TGF-β (10 ng/ml).
T306 5877-5937 Sentence denotes Each day, the TAT proteins were freshly added to the medium.
T307 5938-6004 Sentence denotes FOXP3 expression was measured after 5 d by intracellular staining.
T308 6006-6038 Sentence denotes Intracellular cytokine staining.
T309 6039-6134 Sentence denotes T cells were stimulated with 2 × 10−7 M PMA and 1 μg/ml of ionomycin (Sigma Chemicals) for 4 h.
T310 6135-6188 Sentence denotes The following mAb was used: anti-IL-4-PE (8D4–8, BD).
T311 6189-6287 Sentence denotes Matched isotype controls were used at the same protein concentration as the respective antibodies.
T312 6288-6428 Sentence denotes Four-color FACS was performed using an EPICS XL-MCL (Beckman Coulter) using the software Expo32 version for data acquisition and evaluation.
T313 6430-6445 Sentence denotes Flow cytometry.
T314 6446-6804 Sentence denotes For analysis of FOXP3 expression at the single-cell level, cells were first stained with the monoclonal antibody CD25 (Beckman Coulter), and after fixation and permeabilization, cells were incubated with PE-conjugated monoclonal antibody PCH101 (anti-human FOXP3; eBioscience) based on the manufacturer's recommendations and subjected to FACS (EPICS XL-MCL).
T315 6805-7130 Sentence denotes For cell surface marker staining, cells were incubated for 20 min at 4°C in staining buffer with the following antibodies: anti–CD152-PE (CTLA-4; BD), anti–PD-1 (eBiosciences), anti-GITR (R & D Systems), anti-CD69 (Beckman Coulter), anti-CD103 (DakoCytomation), anti-CD62L (Beckman Coulter), or anti-HLA-DR (Beckman Coulter).
T316 7131-7201 Sentence denotes The controls were FITC, PE, or ECD-conjugated mouse IgG1 or rat IgG2a.
T317 7202-7364 Sentence denotes For staining of mouse cells, the following mAbs from BD Biosciences were used following standard techniques as described above: anti-CD3, anti-CD4, and anti-CD25.
T318 7365-7473 Sentence denotes Anti-FcγRII/III antibody (2.4G2, ATCC) was included in all stainings to reduce nonspecific antibody binding.
T319 7474-7649 Sentence denotes To isolate naive murine CD4 T cells from murine DO11.10 or DO11.10xCD2-GATA3 T cells, cells were stained with anti-CD25-FITC, anti-CD62L-PE, and anti-CD4-APC prior to sorting.
T320 7650-7718 Sentence denotes Dead cells were excluded with 4′',6-Diamidino-2-phenylindole (DAPI).
T321 7719-7992 Sentence denotes To analyze murine Foxp3 expression in inducible Treg cultures, cells were stained intracellularly with anti-Foxp3-PE according to manufacturer's instruction, in conjunction with anti-CD4-APC and LIVE/DEAD fixable dead cell stain kit (Invitrogen) to discriminate live cells.
T322 7993-8095 Sentence denotes All monoclonal antibodies for murine cell stainings were purchased from eBioscience or BD Biosciences.
T323 8097-8114 Sentence denotes Western blotting.
T324 8115-8300 Sentence denotes For FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen).
T325 8301-8379 Sentence denotes The proteins were electroblotted onto a PVDF membrane (Amersham Life Science).
T326 8380-8554 Sentence denotes Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C.
T327 8555-8683 Sentence denotes The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji).
T328 8684-8752 Sentence denotes Membranes were incubated in stripping buffer and re-blocked for 1 h.
T329 8753-9003 Sentence denotes The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),
T330 9005-9056 Sentence denotes Administration of cytokines and antibodies in vivo.
T331 9057-9296 Sentence denotes Age- and gender-matched normal B6 mice received every other day intraperitoneal (ip) injections of PBS, 1.5 μg rmIL-4, 50 μg anti-IL-4 mAb (11B11 or MAB404), or a mixture of 1.5 μg rmIL-4 plus 50 μg anti-IL-4 mAb (11B11 or MAB404) for 7 d.
T332 9297-9403 Sentence denotes Thereafter, spleen and lymph node cells were analyzed by flow cytometry for CD3, CD4, and CD25 expression.
T333 9404-9534 Sentence denotes The anti-mouse IL-4 mAb MAB404 was obtained from R&D Systems, the second anti-mouse IL-4 mAb 11B11 was purchased from eBioscience.
T334 9536-9541 Sentence denotes ChIP.
T335 9542-9752 Sentence denotes ChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature.
T336 9753-9867 Sentence denotes The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin).
T337 9868-10026 Sentence denotes The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h.
T338 10027-10116 Sentence denotes Washing and elution buffers were used according to the protocol of Upstate Biotechnology.
T339 10117-10261 Sentence denotes Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction.
T340 10262-10315 Sentence denotes The amount of DNA was determined by conventional PCR.
T341 10316-10423 Sentence denotes The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers:
T342 10424-10485 Sentence denotes 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′.
T343 10486-10549 Sentence denotes The PCR products were visualized using an ethidium bromide gel.
T344 10551-10567 Sentence denotes Pull-down assay.
T345 10568-10636 Sentence denotes CD4+ T cells were stimulated with PMA and ionomycin for 2 h at 37°C.
T346 10637-10832 Sentence denotes The cells were pelleted, resuspended in buffer C (20 mM HEPES [pH 7.9], 420 mM NaCl, 1.5 mM MgCl2, 0.2 mM EDTA, 1 mM DTT, protease inhibitors [Sigma]. and 0.1% NP-40) and lysed on ice for 15 min.
T347 10833-10882 Sentence denotes Insoluble material was removed by centrifugation.
T348 10883-10961 Sentence denotes The supernatant was diluted 1:3 with buffer D (as buffer C, but without NaCl).
T349 10962-11136 Sentence denotes The lysates were incubated with 10 μg of poly(dI-dC) (Sigma) and 70 μl of streptavidin-agarose (Amersham Biosciences) carrying biotinylated oligonucleotides, for 3 h at 4 °C.
T350 11137-11346 Sentence denotes The beads were washed twice with buffer C:D (1:3) and resuspended in DTT-containing loading buffer (NuPAGE; Invitrogen), heated to 70 °C for 10 min, and the eluants on a NuPAGE 4–12% bis-tris gel (Invitrogen).
T351 11347-11488 Sentence denotes The proteins were electroblotted onto a PVDF membrane (Amersham Biosciences) and detected using an anti-GATA3 mAb (Santa Cruz Biotechnology).
T352 11489-11553 Sentence denotes Accumulated signals were analyzed using AIDA software (Raytest).

events-check-again

Id Subject Object Predicate Lexical cue Speculation
T18037 238-248 Gene_expression denotes expressing
T18038 249-255 Protein denotes GATA-3
T18039 277-280 Protein denotes CD2
T18040 303-306 Protein denotes CD2
T18041 307-312 Protein denotes GATA3
T18042 341-344 Protein denotes CD2
T18043 345-350 Protein denotes GATA3
T18746 1241-1246 Protein denotes FOXP3
T18747 1300-1305 Protein denotes FOXP3
T18748 1351-1356 Protein denotes EF-1α
T18749 1410-1415 Protein denotes GATA3
T18750 1829-1840 Protein denotes Ubiquitin C
T18751 1914-1920 Protein denotes Smad-7
T18752 1993-1999 Protein denotes TGF-ß1
T18753 2420-2431 Protein denotes ubiquitin C
T19471 3175-3178 Protein denotes CD4
T19472 3180-3186 Protein denotes CD45RA
T19473 3209-3215 Protein denotes CD45RO
T19474 3216-3225 Negative_regulation denotes depletion
T19475 3336-3339 Protein denotes CD3
T19476 3371-3375 Protein denotes CD28
T19477 3414-3419 Protein denotes IL-12
T19478 3434-3438 Protein denotes IL-4
T19479 3482-3486 Protein denotes IL-4
T19480 3501-3506 Protein denotes IFN-γ
T19481 3521-3526 Protein denotes IL-12
T19482 3574-3579 Protein denotes TGF-β
T19483 3594-3599 Protein denotes IFN-γ
T19484 3614-3619 Protein denotes IL-12
T19485 3634-3638 Protein denotes IL-4
T19486 3695-3699 Protein denotes IL-2
T20691 5850-5854 Protein denotes CD28
T20692 5859-5864 Protein denotes TGF-β
T20693 5891-5894 Protein denotes TAT
T20694 5938-5943 Protein denotes FOXP3
T20695 5944-5954 Gene_expression denotes expression true
T20887 6168-6172 Protein denotes IL-4
T21708 6462-6467 Protein denotes FOXP3
T21709 6468-6478 Gene_expression denotes expression true
T21710 6559-6563 Protein denotes CD25
T21711 6703-6708 Protein denotes FOXP3
T21712 6933-6938 Protein denotes CD152
T21713 6961-6965 Protein denotes PD-1
T21714 6987-6991 Protein denotes GITR
T21715 7014-7018 Protein denotes CD69
T21716 7043-7048 Protein denotes CD103
T21717 7072-7077 Protein denotes CD62L
T21718 7335-7338 Protein denotes CD3
T21719 7345-7348 Protein denotes CD4
T21720 7359-7363 Protein denotes CD25
T21721 7498-7501 Protein denotes CD4
T21722 7541-7544 Protein denotes CD2
T21723 7545-7550 Protein denotes GATA3
T21724 7589-7593 Protein denotes CD25
T21725 7605-7610 Protein denotes CD62L
T21726 7624-7627 Protein denotes CD4
T21727 7737-7742 Protein denotes Foxp3
T21728 7743-7753 Gene_expression denotes expression true
T21729 7827-7832 Protein denotes Foxp3
T21730 7902-7905 Protein denotes CD4
T22201 8119-8124 Protein denotes FOXP3
T22202 8164-8167 Protein denotes CD4
T22203 8168-8172 Protein denotes CD25
T22204 8503-8508 Protein denotes FOXP3
T22205 8793-8798 Protein denotes GATA3
T22206 8840-8845 Protein denotes T-bet
T22207 8885-8890 Protein denotes GAPDH
T22208 8919-8924 Protein denotes SMAD2
T22209 8947-8952 Protein denotes STAT6
T22210 8969-8974 Protein denotes STAT6
T22537 9187-9191 Protein denotes IL-4
T22538 9240-9244 Protein denotes IL-4
T22539 9261-9265 Protein denotes IL-4
T22540 9373-9376 Protein denotes CD3
T22541 9378-9381 Protein denotes CD4
T22542 9387-9391 Protein denotes CD25
T22543 9392-9402 Gene_expression denotes expression true
T22544 9392-9402 Gene_expression denotes expression true
T22545 9392-9402 Gene_expression denotes expression true
T22546 9419-9423 Protein denotes IL-4
T22547 9488-9492 Protein denotes IL-4
T22957 9963-9968 Protein denotes GATA3
T22958 10342-10347 Protein denotes FOXP3
T23424 10568-10571 Protein denotes CD4
T23425 11451-11456 Protein denotes GATA3
T18290 647-650 Protein denotes CD4
T18291 661-664 Protein denotes CD4
T18292 742-745 Protein denotes CD4
T18293 831-834 Protein denotes CD4
T18294 1029-1033 Positive_regulation denotes high
T18295 1034-1038 Protein denotes IL-4
T18296 1039-1048 Gene_expression denotes secretion
T19044 2672-2675 Protein denotes CD4
T19045 2686-2689 Protein denotes CD4
T19046 2692-2697 Protein denotes CD62L
T19047 2704-2708 Protein denotes CD25
T19048 2972-2978 Protein denotes TGF-ß1
T19049 3073-3078 Protein denotes FOXP3
T19050 3079-3089 Gene_expression denotes expression true
T19733 3728-3733 Protein denotes FOXP3
T19734 3786-3791 Protein denotes FOXP3
T19735 3878-3883 Protein denotes FOXP3
T19736 3933-3938 Protein denotes FOXP3
T20030 4319-4324 Protein denotes FOXP3
T20031 4334-4344 Protein denotes luciferase
T20032 4402-4405 Protein denotes CD4
T20033 4631-4641 Protein denotes luciferase
T20034 4676-4686 Protein denotes luciferase
T20035 4812-4822 Protein denotes luciferase
T20392 5146-5149 Protein denotes TAT
T20393 5179-5184 Protein denotes GATA3
T20394 5210-5215 Protein denotes GATA3
T20395 5217-5224 Negative_regulation denotes lacking
T20396 5229-5245 Entity denotes two zinc fingers
T20397 5309-5312 Protein denotes TAT
T20398 5483-5486 Protein denotes TAT
T20685 5599-5602 Protein denotes TAT
T20686 5603-5608 Protein denotes GATA3
T20687 5623-5626 Protein denotes CD4
T20688 5627-5633 Protein denotes CD45RA
T20689 5742-5747 Protein denotes GATA3
T20690 5837-5840 Protein denotes CD3
R13641 T18038 T18037 themeOf GATA-3,expressing
R13821 T18295 T18296 themeOf IL-4,secretion
R13822 T18296 T18294 themeOf secretion,high
R14366 T19049 T19050 themeOf FOXP3,expression
R14621 T19473 T19474 themeOf CD45RO,depletion
R15284 T20396 T20395 themeOf two zinc fingers,lacking
R15285 T20396 T20394 partOf two zinc fingers,GATA3
R15456 T20694 T20695 themeOf FOXP3,expression
R16208 T21708 T21709 themeOf FOXP3,expression
R16209 T21727 T21728 themeOf Foxp3,expression
R16791 T22540 T22545 themeOf CD3,expression
R16792 T22541 T22544 themeOf CD4,expression
R16793 T22542 T22543 themeOf CD25,expression

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T18010 271-301 Protein denotes human CD2 locus control region
T18011 303-306 Protein denotes CD2
T18012 307-312 Protein denotes GATA3
T18013 333-350 Protein denotes DO11.10xCD2-GATA3
T18014 238-248 Gene_expression denotes expressing
T18015 238-248 Gene_expression denotes expressing
T18711 1155-1162 Protein denotes GenBank
T18712 1241-1246 Protein denotes FOXP3
T18713 1269-1284 Protein denotes ACA GCA CAT TCC
T18714 1285-1292 Protein denotes CAG AGT
T18715 1300-1305 Protein denotes FOXP3
T18716 1351-1356 Protein denotes EF-1α
T18717 1410-1415 Protein denotes GATA3
T18718 1635-1644 Protein denotes ABI PRISM
T18719 1829-1840 Protein denotes Ubiquitin C
T18720 1993-1999 Protein denotes TGF-ß1
T18721 2420-2431 Protein denotes ubiquitin C
T19414 3175-3178 Protein denotes CD4
T19415 3180-3187 Protein denotes CD45RA+
T19416 3209-3215 Protein denotes CD45RO
T19417 3350-3354 Protein denotes Okt3
T19418 3356-3360 Protein denotes IgG1
T19419 3389-3392 Protein denotes Th1
T19420 3496-3506 Protein denotes anti-IFN-γ
T19421 3589-3599 Protein denotes anti-IFN-γ
T19422 3216-3225 Negative_regulation denotes depletion
T19714 3728-3742 Protein denotes FOXP3 promoter
T19715 3786-3800 Protein denotes FOXP3 promoter
T19716 3873-3877 Protein denotes pGL3
T19717 3878-3883 Protein denotes FOXP3
T19718 3933-3947 Protein denotes FOXP3 promoter
T19719 4118-4133 Protein denotes GTT TCT CAT GAG
T19720 4134-4184 Protein denotes CCC TAT TAA GTC ATT CTT ACC TCT CAC CTC TGT GGT GA
T20641 5599-5602 Protein denotes TAT
T20642 5603-5608 Protein denotes GATA3
T20643 5623-5626 Protein denotes CD4
T20644 5627-5633 Protein denotes CD45RA
T20645 5742-5747 Protein denotes GATA3
T20646 5832-5840 Protein denotes anti-CD3
T20647 5845-5854 Protein denotes anti-CD28
T20648 5859-5864 Protein denotes TGF-β
T20649 5891-5903 Protein denotes TAT proteins
T20650 5938-5943 Protein denotes FOXP3
T20651 5944-5954 Gene_expression denotes expression
T21619 6462-6467 Protein denotes FOXP3
T21620 6468-6478 Gene_expression denotes expression
T21621 7072-7077 Protein denotes CD62L
T21622 7162-7187 Protein denotes ECD-conjugated mouse IgG1
T21623 7191-7200 Protein denotes rat IgG2a
T21624 7335-7338 Protein denotes CD3
T21625 7345-7348 Protein denotes CD4
T21626 7359-7363 Protein denotes CD25
T21627 7365-7389 Protein denotes Anti-FcγRII/III antibody
T21628 7498-7503 Protein denotes CD4 T
T21629 7605-7610 Protein denotes CD62L
T21630 7611-7613 Protein denotes PE
T21631 7624-7627 Protein denotes CD4
T21632 7628-7631 Protein denotes APC
T21633 7737-7742 Protein denotes Foxp3
T21634 7902-7905 Protein denotes CD4
T21635 7906-7909 Protein denotes APC
T21636 7914-7918 Protein denotes LIVE
T21637 7919-7923 Protein denotes DEAD
T21638 7743-7753 Gene_expression denotes expression
T22162 8119-8124 Protein denotes FOXP3
T22163 8164-8167 Protein denotes CD4
T22164 8168-8172 Protein denotes CD25
T22165 8493-8508 Protein denotes goat anti-FOXP3
T22166 8611-8614 Protein denotes mAb
T22167 8964-8974 Protein denotes anti-STAT6
T22499 9182-9213 Protein denotes anti-IL-4 mAb (11B11 or MAB404)
T22500 9373-9376 Protein denotes CD3
T22501 9378-9381 Protein denotes CD4
T22502 9387-9391 Protein denotes CD25
T22503 9392-9402 Gene_expression denotes expression
T22504 9392-9402 Gene_expression denotes expression
T22505 9392-9402 Gene_expression denotes expression
T22506 9408-9434 Protein denotes anti-mouse IL-4 mAb MAB404
T22507 9477-9502 Protein denotes anti-mouse IL-4 mAb 11B11
T22949 9924-9927 Protein denotes IgG
T20369 5238-5245 Entity denotes fingers
T18262 647-651 Protein denotes CD4+
T18263 661-665 Protein denotes CD4+
T18264 831-835 Protein denotes CD4+
T18265 979-985 Protein denotes Vbeta8
T18266 986-994 Gene_expression denotes positive
T18267 1034-1038 Protein denotes IL-4
T18268 1039-1048 Localization denotes secretion
T19016 2672-2676 Protein denotes CD4+
T19017 2686-2689 Protein denotes CD4
T19018 2692-2697 Protein denotes CD62L
T19019 2704-2709 Protein denotes CD25–
T19020 2970-2978 Protein denotes rhTGF-ß1
T19021 3073-3078 Protein denotes FOXP3
T19022 3079-3089 Gene_expression denotes expression
T20008 4319-4353 Protein denotes FOXP3 promoter luciferase reporter
T20009 4631-4641 Protein denotes luciferase
T20010 4676-4686 Protein denotes luciferase
T20011 4804-4822 Protein denotes renilla luciferase
T20149 5011-5017 Protein denotes TaqMan
T20365 5134-5158 Protein denotes Recombinant TAT proteins
T20366 5179-5192 Protein denotes GATA3 protein
T20367 5210-5215 Protein denotes GATA3
T20368 5309-5321 Protein denotes TAT sequence
T20370 5217-5224 Negative_regulation denotes lacking
T20371 5483-5486 Protein denotes TAT
T22950 10342-10356 Protein denotes FOXP3 promoter
T23415 10568-10572 Protein denotes CD4+
T23416 10751-10778 Protein denotes mM DTT, protease inhibitors
T23417 10780-10785 Protein denotes Sigma
R13635 T18010 T18014 themeOf human CD2 locus control region,expressing
R13636 T18012 T18015 themeOf GATA3,expressing
R13813 T18265 T18266 themeOf Vbeta8,positive
R13814 T18267 T18268 themeOf IL-4,secretion
R14362 T19021 T19022 themeOf FOXP3,expression
R14617 T19416 T19422 themeOf CD45RO,depletion
R15277 T20367 T20370 themeOf GATA3,lacking
R15278 T20367 T20369 partOf GATA3,fingers
R15279 T20369 T20370 Site fingers,lacking
R15452 T20650 T20651 themeOf FOXP3,expression
R16200 T21619 T21620 themeOf FOXP3,expression
R16201 T21633 T21638 themeOf Foxp3,expression
R16780 T22500 T22503 themeOf CD3,expression
R16781 T22501 T22504 themeOf CD4,expression
R16782 T22502 T22505 themeOf CD25,expression

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Speculation
T18780 2672-2675 Protein denotes CD4
T18781 2686-2689 Protein denotes CD4
T18782 2692-2697 Protein denotes CD62L
T18783 2704-2708 Protein denotes CD25
T18784 2972-2978 Protein denotes TGF-ß1
T18785 3073-3078 Protein denotes FOXP3
T18786 3079-3089 Gene_expression denotes expression true
T20428 5599-5602 Protein denotes TAT
T20429 5603-5608 Protein denotes GATA3
T20430 5623-5626 Protein denotes CD4
T20431 5627-5633 Protein denotes CD45RA
T20432 5742-5747 Protein denotes GATA3
T20433 5837-5840 Protein denotes CD3
T20434 5850-5854 Protein denotes CD28
T20435 5859-5864 Protein denotes TGF-β
T20436 5891-5894 Protein denotes TAT
T20437 5938-5943 Protein denotes FOXP3
T20438 5944-5954 Gene_expression denotes expression true
T21774 8119-8124 Protein denotes FOXP3
T21775 8164-8167 Protein denotes CD4
T21776 8168-8172 Protein denotes CD25
T21777 8503-8508 Protein denotes FOXP3
T21778 8793-8798 Protein denotes GATA3
T21779 8840-8845 Protein denotes T-bet
T21780 8885-8890 Protein denotes GAPDH
T21781 8919-8924 Protein denotes SMAD2
T21782 8947-8952 Protein denotes STAT6
T21783 8969-8974 Protein denotes STAT6
T22563 9963-9968 Protein denotes GATA3
T22564 10342-10347 Protein denotes FOXP3
T22965 10568-10571 Protein denotes CD4
T22966 11451-11456 Protein denotes GATA3
T19755 4319-4324 Protein denotes FOXP3
T19756 4334-4344 Protein denotes luciferase
T19757 4402-4405 Protein denotes CD4
T19758 4631-4641 Protein denotes luciferase
T19759 4676-4686 Protein denotes luciferase
T19760 4812-4822 Protein denotes luciferase
T20709 6168-6172 Protein denotes IL-4
T18066 647-650 Protein denotes CD4
T18067 661-664 Protein denotes CD4
T18068 742-745 Protein denotes CD4
T18069 831-834 Protein denotes CD4
T18070 1029-1033 Positive_regulation denotes high
T18071 1034-1038 Protein denotes IL-4
T18072 1039-1048 Gene_expression denotes secretion
T22244 9187-9191 Protein denotes IL-4
T22245 9240-9244 Protein denotes IL-4
T22246 9261-9265 Protein denotes IL-4
T22247 9373-9376 Protein denotes CD3
T22248 9378-9381 Protein denotes CD4
T22249 9387-9391 Protein denotes CD25
T22250 9392-9402 Gene_expression denotes expression true
T22251 9392-9402 Gene_expression denotes expression true
T22252 9392-9402 Gene_expression denotes expression true
T22253 9419-9423 Protein denotes IL-4
T22254 9488-9492 Protein denotes IL-4
T20164 5146-5149 Protein denotes TAT
T20165 5179-5184 Protein denotes GATA3
T20166 5210-5215 Protein denotes GATA3
T20167 5217-5224 Negative_regulation denotes lacking
T20168 5229-5245 Entity denotes two zinc fingers
T20169 5309-5312 Protein denotes TAT
T20170 5483-5486 Protein denotes TAT
T19512 3728-3733 Protein denotes FOXP3
T19513 3786-3791 Protein denotes FOXP3
T19514 3878-3883 Protein denotes FOXP3
T19515 3933-3938 Protein denotes FOXP3
T19090 3175-3178 Protein denotes CD4
T19091 3180-3186 Protein denotes CD45RA
T19092 3209-3215 Protein denotes CD45RO
T19093 3216-3225 Negative_regulation denotes depletion
T19094 3336-3339 Protein denotes CD3
T19095 3371-3375 Protein denotes CD28
T19096 3414-3419 Protein denotes IL-12
T19097 3434-3438 Protein denotes IL-4
T19098 3482-3486 Protein denotes IL-4
T19099 3501-3506 Protein denotes IFN-γ
T19100 3521-3526 Protein denotes IL-12
T19101 3574-3579 Protein denotes TGF-β
T19102 3594-3599 Protein denotes IFN-γ
T19103 3614-3619 Protein denotes IL-12
T19104 3634-3638 Protein denotes IL-4
T19105 3695-3699 Protein denotes IL-2
T18320 1241-1246 Protein denotes FOXP3
T18321 1300-1305 Protein denotes FOXP3
T18322 1351-1356 Protein denotes EF-1α
T18323 1410-1415 Protein denotes GATA3
T18324 1829-1840 Protein denotes Ubiquitin C
T18325 1914-1920 Protein denotes Smad-7
T18326 1993-1999 Protein denotes TGF-ß1
T18327 2420-2431 Protein denotes ubiquitin C
T17765 238-248 Gene_expression denotes expressing
T17766 249-255 Protein denotes GATA-3
T17767 277-280 Protein denotes CD2
T17768 303-306 Protein denotes CD2
T17769 307-312 Protein denotes GATA3
T17770 341-344 Protein denotes CD2
T17771 345-350 Protein denotes GATA3
T20935 6462-6467 Protein denotes FOXP3
T20936 6468-6478 Gene_expression denotes expression true
T20937 6559-6563 Protein denotes CD25
T20938 6703-6708 Protein denotes FOXP3
T20939 6933-6938 Protein denotes CD152
T20940 6961-6965 Protein denotes PD-1
T20941 6987-6991 Protein denotes GITR
T20942 7014-7018 Protein denotes CD69
T20943 7043-7048 Protein denotes CD103
T20944 7072-7077 Protein denotes CD62L
T20945 7335-7338 Protein denotes CD3
T20946 7345-7348 Protein denotes CD4
T20947 7359-7363 Protein denotes CD25
T20948 7498-7501 Protein denotes CD4
T20949 7541-7544 Protein denotes CD2
T20950 7545-7550 Protein denotes GATA3
T20951 7589-7593 Protein denotes CD25
T20952 7605-7610 Protein denotes CD62L
T20953 7624-7627 Protein denotes CD4
T20954 7737-7742 Protein denotes Foxp3
T20955 7743-7753 Gene_expression denotes expression true
T20956 7827-7832 Protein denotes Foxp3
T20957 7902-7905 Protein denotes CD4
R15290 T20437 T20438 themeOf FOXP3,expression
R14158 T18785 T18786 themeOf FOXP3,expression
R13647 T18071 T18072 themeOf IL-4,secretion
R13648 T18072 T18070 themeOf secretion,high
R16567 T22247 T22252 themeOf CD3,expression
R16568 T22248 T22251 themeOf CD4,expression
R16569 T22249 T22250 themeOf CD25,expression
R15109 T20168 T20167 themeOf two zinc fingers,lacking
R15110 T20168 T20166 partOf two zinc fingers,GATA3
R14370 T19092 T19093 themeOf CD45RO,depletion
R13417 T17766 T17765 themeOf GATA-3,expressing
R15617 T20935 T20936 themeOf FOXP3,expression
R15618 T20954 T20955 themeOf Foxp3,expression

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T18887 2672-2675 P01730 denotes CD4
T18888 2686-2689 P01730 denotes CD4
T18889 2692-2697 P14151 denotes CD62L
T18890 2704-2708 P01589 denotes CD25
T18891 3073-3078 Q9BZS1 denotes FOXP3
T19228 3175-3178 P01730 denotes CD4
T19229 3180-3186 P08575 denotes CD45RA
T19230 3209-3215 P08575 denotes CD45RO
T19231 3336-3339 P07766 denotes CD3
T19232 3336-3339 P20963 denotes CD3
T19233 3336-3339 P09693 denotes CD3
T19234 3336-3339 P04234 denotes CD3
T19235 3371-3375 P10747 denotes CD28
T19236 3414-3419 P29460 denotes IL-12
T19237 3414-3419 P29459 denotes IL-12
T19238 3434-3438 P05112 denotes IL-4
T19239 3482-3486 P05112 denotes IL-4
T19240 3501-3506 P01579 denotes IFN-γ
T19241 3521-3526 P29460 denotes IL-12
T19242 3521-3526 P29459 denotes IL-12
T19243 3574-3579 P01137 denotes TGF-β
T19244 3594-3599 P01579 denotes IFN-γ
T19245 3614-3619 P29459 denotes IL-12
T19246 3614-3619 P29460 denotes IL-12
T19247 3634-3638 P05112 denotes IL-4
T19248 3695-3699 P60568 denotes IL-2
T19874 4261-4264 P26358 denotes AIM
T19875 4319-4324 Q9BZS1 denotes FOXP3
T19876 4334-4344 P08659 denotes luciferase
T19877 4402-4405 P01730 denotes CD4
T19878 4631-4641 P08659 denotes luciferase
T19879 4676-4686 P08659 denotes luciferase
T19880 4812-4822 P08659 denotes luciferase
T20254 5146-5149 P04605 denotes TAT
T20255 5146-5149 P04608 denotes TAT
T20256 5146-5149 P04610 denotes TAT
T20257 5146-5149 Q8UMQ1 denotes TAT
T20258 5179-5184 P23771 denotes GATA3
T20259 5210-5215 P23771 denotes GATA3
T20260 5309-5312 P04610 denotes TAT
T20261 5309-5312 Q8UMQ1 denotes TAT
T20262 5309-5312 P04608 denotes TAT
T20263 5309-5312 P04605 denotes TAT
T20264 5483-5486 Q8UMQ1 denotes TAT
T20265 5483-5486 P04610 denotes TAT
T20266 5483-5486 P04608 denotes TAT
T20267 5483-5486 P04605 denotes TAT
T21956 8119-8124 Q9BZS1 denotes FOXP3
T21957 8164-8167 P01730 denotes CD4
T21958 8168-8172 P01589 denotes CD25
T21959 8503-8508 Q9BZS1 denotes FOXP3
T21960 8793-8798 P23771 denotes GATA3
T21961 8840-8845 Q9UL17 denotes T-bet
T21962 8885-8890 P04406 denotes GAPDH
T21963 8919-8924 Q15796 denotes SMAD2
T21964 8947-8952 P42226 denotes STAT6
T21965 8969-8974 P42226 denotes STAT6
T22362 9187-9191 P05112 denotes IL-4
T22363 9261-9265 P05112 denotes IL-4
T22364 9373-9376 P20963 denotes CD3
T22365 9373-9376 P09693 denotes CD3
T22366 9373-9376 P04234 denotes CD3
T22367 9373-9376 P07766 denotes CD3
T22368 9378-9381 P01730 denotes CD4
T22369 9387-9391 P01589 denotes CD25
T22370 9419-9423 P05112 denotes IL-4
T22371 9488-9492 P05112 denotes IL-4
T23182 10568-10571 P01730 denotes CD4
T23183 10700-10702 P0A7Z4 denotes pH
T23184 11451-11456 P23771 denotes GATA3
T17881 249-255 P23771 denotes GATA-3
T17882 277-280 P06729 denotes CD2
T17883 303-306 P06729 denotes CD2
T17884 307-312 P23771 denotes GATA3
T17885 345-350 P23771 denotes GATA3
T18343 1241-1246 Q9BZS1 denotes FOXP3
T18344 1300-1305 Q9BZS1 denotes FOXP3
T18345 1410-1415 P23771 denotes GATA3
T18346 1949-1952 P04608 denotes TAT
T18347 1949-1952 P04610 denotes TAT
T18348 1949-1952 Q8UMQ1 denotes TAT
T18349 1949-1952 P04605 denotes TAT
T18350 1967-1970 P04591 denotes GAG
T18351 2068-2071 P21980 denotes TGC
T18352 2518-2526 Q04864 denotes Relative
T21245 6462-6467 Q9BZS1 denotes FOXP3
T21246 6559-6563 P01589 denotes CD25
T21247 6703-6708 Q9BZS1 denotes FOXP3
T21248 7072-7077 P14151 denotes CD62L
T21249 7335-7338 P09693 denotes CD3
T21250 7335-7338 P07766 denotes CD3
T21251 7335-7338 P04234 denotes CD3
T21252 7335-7338 P20963 denotes CD3
T21253 7345-7348 P01730 denotes CD4
T21254 7359-7363 P01589 denotes CD25
T21255 7498-7501 P01730 denotes CD4
T21256 7545-7550 P23771 denotes GATA3
T21257 7589-7593 P01589 denotes CD25
T21258 7605-7610 P14151 denotes CD62L
T21259 7624-7627 P01730 denotes CD4
T21260 7737-7742 Q9BZS1 denotes Foxp3
T21261 7827-7832 Q9BZS1 denotes Foxp3
T21262 7902-7905 P01730 denotes CD4
T18156 647-650 P01730 denotes CD4
T18157 661-664 P01730 denotes CD4
T18158 742-745 P01730 denotes CD4
T18159 831-834 P01730 denotes CD4
T18160 1034-1038 P05112 denotes IL-4
T19605 3728-3733 Q9BZS1 denotes FOXP3
T19606 3786-3791 Q9BZS1 denotes FOXP3
T19607 3878-3883 Q9BZS1 denotes FOXP3
T19608 3933-3938 Q9BZS1 denotes FOXP3
T19609 4130-4133 P04591 denotes GAG
T19610 4138-4141 Q8UMQ1 denotes TAT
T19611 4138-4141 P04610 denotes TAT
T19612 4138-4141 P04608 denotes TAT
T19613 4138-4141 P04605 denotes TAT
T20520 5599-5602 Q8UMQ1 denotes TAT
T20521 5599-5602 P04608 denotes TAT
T20522 5599-5602 P04610 denotes TAT
T20523 5599-5602 P04605 denotes TAT
T20524 5603-5608 P23771 denotes GATA3
T20525 5623-5626 P01730 denotes CD4
T20526 5627-5633 P08575 denotes CD45RA
T20527 5742-5747 P23771 denotes GATA3
T20528 5837-5840 P07766 denotes CD3
T20529 5837-5840 P04234 denotes CD3
T20530 5837-5840 P09693 denotes CD3
T20531 5837-5840 P20963 denotes CD3
T20532 5850-5854 P10747 denotes CD28
T20533 5859-5864 P01137 denotes TGF-β
T20534 5891-5894 Q8UMQ1 denotes TAT
T20535 5891-5894 P04610 denotes TAT
T20536 5891-5894 P04608 denotes TAT
T20537 5891-5894 P04605 denotes TAT
T20538 5938-5943 Q9BZS1 denotes FOXP3
T20788 6168-6172 P05112 denotes IL-4
T22743 9963-9968 P23771 denotes GATA3
T22744 10342-10347 Q9BZS1 denotes FOXP3

test2

Id Subject Object Predicate Lexical cue Speculation
T17758 238-248 Gene_expression denotes expressing
T17759 249-255 Protein denotes GATA-3
T17760 277-280 Protein denotes CD2
T17761 303-306 Protein denotes CD2
T17762 307-312 Protein denotes GATA3
T17763 338-344 Protein denotes 10xCD2
T17764 345-350 Protein denotes GATA3
T18058 647-650 Protein denotes CD4
T18059 661-664 Protein denotes CD4
T18060 742-745 Protein denotes CD4
T18061 831-834 Protein denotes CD4
T18062 1029-1033 Positive_regulation denotes high
T18063 1034-1038 Protein denotes IL-4
T18064 1039-1048 Localization denotes secretion
T18312 1241-1246 Protein denotes FOXP3
T18313 1300-1305 Protein denotes FOXP3
T18314 1351-1356 Protein denotes EF-1α
T18315 1410-1415 Protein denotes GATA3
T18316 1829-1840 Protein denotes Ubiquitin C
T18317 1914-1920 Protein denotes Smad-7
T18318 1993-1999 Protein denotes TGF-ß1
T18319 2420-2431 Protein denotes ubiquitin C
T18769 2672-2675 Protein denotes CD4 true
T18770 2686-2689 Protein denotes CD4
T18771 2692-2697 Protein denotes CD62L
T18772 2704-2708 Protein denotes CD25
T18773 2975-2978 Protein denotes -ß1
T18774 3073-3078 Protein denotes FOXP3
T18775 3079-3089 Gene_expression denotes expression
T19074 3175-3178 Protein denotes CD4
T19075 3180-3186 Protein denotes CD45RA
T19076 3209-3215 Protein denotes CD45RO
T19077 3216-3225 Negative_regulation denotes depletion
T19078 3336-3339 Protein denotes CD3
T19079 3371-3375 Protein denotes CD28
T19080 3414-3419 Protein denotes IL-12
T19081 3434-3438 Protein denotes IL-4
T19082 3482-3486 Protein denotes IL-4
T19083 3501-3506 Protein denotes IFN-γ
T19084 3521-3526 Protein denotes IL-12
T19085 3574-3579 Protein denotes TGF-β
T19086 3594-3599 Protein denotes IFN-γ
T19087 3614-3619 Protein denotes IL-12
T19088 3634-3638 Protein denotes IL-4
T19089 3695-3699 Protein denotes IL-2
T19507 3728-3733 Protein denotes FOXP3
T19508 3786-3791 Protein denotes FOXP3
T19509 3792-3800 Entity denotes promoter
T19510 3878-3883 Protein denotes FOXP3
T19511 3933-3938 Protein denotes FOXP3
T19747 4319-4324 Protein denotes FOXP3
T19748 4334-4344 Protein denotes luciferase
T19749 4402-4405 Protein denotes CD4
T19750 4631-4641 Protein denotes luciferase
T19751 4676-4686 Protein denotes luciferase
T19752 4812-4822 Protein denotes luciferase
T20157 5146-5149 Protein denotes TAT
T20158 5179-5184 Protein denotes GATA3
T20159 5210-5215 Protein denotes GATA3
T20160 5217-5224 Negative_regulation denotes lacking
T20161 5229-5245 Entity denotes two zinc fingers
T20162 5309-5312 Protein denotes TAT
T20163 5483-5486 Protein denotes TAT
T20417 5599-5602 Protein denotes TAT true
T20418 5603-5608 Protein denotes GATA3
T20419 5623-5626 Protein denotes CD4
T20420 5627-5633 Protein denotes CD45RA
T20421 5742-5747 Protein denotes GATA3
T20422 5837-5840 Protein denotes CD3
T20423 5850-5854 Protein denotes CD28
T20424 5859-5864 Protein denotes TGF-β
T20425 5891-5894 Protein denotes TAT
T20426 5938-5943 Protein denotes FOXP3
T20427 5944-5954 Gene_expression denotes expression
T20708 6168-6172 Protein denotes IL-4
T20912 6462-6467 Protein denotes FOXP3 true
T20913 6468-6478 Gene_expression denotes expression true
T20914 6559-6563 Protein denotes CD25
T20915 6703-6708 Protein denotes FOXP3
T20916 6933-6938 Protein denotes CD152
T20917 6961-6965 Protein denotes PD-1
T20918 6987-6991 Protein denotes GITR
T20919 7014-7018 Protein denotes CD69
T20920 7043-7048 Protein denotes CD103
T20921 7072-7077 Protein denotes CD62L
T20922 7335-7338 Protein denotes CD3
T20923 7345-7348 Protein denotes CD4
T20924 7359-7363 Protein denotes CD25
T20925 7498-7501 Protein denotes CD4
T20926 7538-7544 Protein denotes 10xCD2
T20927 7545-7550 Protein denotes GATA3
T20928 7589-7593 Protein denotes CD25
T20929 7605-7610 Protein denotes CD62L
T20930 7624-7627 Protein denotes CD4
T20931 7737-7742 Protein denotes Foxp3
T20932 7743-7753 Gene_expression denotes expression
T20933 7827-7832 Protein denotes Foxp3
T20934 7902-7905 Protein denotes CD4
T21764 8119-8124 Protein denotes FOXP3
T21765 8164-8167 Protein denotes CD4
T21766 8168-8172 Protein denotes CD25
T21767 8503-8508 Protein denotes FOXP3
T21768 8793-8798 Protein denotes GATA3
T21769 8840-8845 Protein denotes T-bet
T21770 8885-8890 Protein denotes GAPDH
T21771 8919-8924 Protein denotes SMAD2
T21772 8947-8952 Protein denotes STAT6
T21773 8969-8974 Protein denotes STAT6
T22232 9187-9191 Protein denotes IL-4 true
T22233 9242-9244 Protein denotes -4 true
T22234 9261-9265 Protein denotes IL-4 true
T22235 9373-9376 Protein denotes CD3
T22236 9378-9381 Protein denotes CD4
T22237 9387-9391 Protein denotes CD25
T22238 9392-9402 Gene_expression denotes expression
T22239 9419-9423 Protein denotes IL-4
T22240 9488-9492 Protein denotes IL-4
T22561 9963-9968 Protein denotes GATA3
T22562 10342-10347 Protein denotes FOXP3
T22963 10568-10571 Protein denotes CD4
T22964 11451-11456 Protein denotes GATA3
R13416 T17759 T17758 themeOf GATA-3,expressing
R13645 T18063 T18064 themeOf IL-4,secretion
R13646 T18064 T18062 themeOf secretion,high
R14157 T18774 T18775 themeOf FOXP3,expression
R14369 T19076 T19077 themeOf CD45RO,depletion
R14623 T19509 T19508 partOf promoter,FOXP3
R15107 T20161 T20160 themeOf two zinc fingers,lacking
R15108 T20161 T20159 partOf two zinc fingers,GATA3
R15289 T20426 T20427 themeOf FOXP3,expression
R15615 T20912 T20913 themeOf FOXP3,expression
R15616 T20931 T20932 themeOf Foxp3,expression
R16564 T22235 T22238 themeOf CD3,expression
R16565 T22236 T22238 themeOf CD4,expression
R16566 T22237 T22238 themeOf CD25,expression