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2_test

Id Subject Object Predicate Lexical cue
18162042-10626890-84704997 3614-3615 10626890 denotes 1
18162042-9846495-84704998 3616-3617 9846495 denotes 2
18162042-16467870-84704999 3667-3668 16467870 denotes 3
18162042-16467870-84705000 3816-3817 16467870 denotes 3
18162042-16520391-84705001 4069-4070 16520391 denotes 4
18162042-15662016-84705002 4071-4072 15662016 denotes 5
18162042-16489117-84705003 4170-4171 16489117 denotes 6
18162042-15634907-84705004 4247-4248 15634907 denotes 7
18162042-10549629-84705005 4304-4305 10549629 denotes 8
18162042-11390432-84705006 4360-4361 11390432 denotes 9
18162042-14676299-84705007 5113-5115 14676299 denotes 10
18162042-15753318-84705008 5283-5285 15753318 denotes 11
18162042-14597769-84705009 5286-5288 14597769 denotes 12
18162042-17185041-84705010 5348-5350 17185041 denotes 13
18162042-17154262-84705011 5351-5353 17154262 denotes 14
18162042-15809351-84705012 5549-5551 15809351 denotes 15
18162042-11045997-84705013 5621-5623 11045997 denotes 16
18162042-15809351-84705014 5734-5736 15809351 denotes 15
18162042-16973386-84705015 5737-5739 16973386 denotes 17
18162042-9881967-84705016 11996-11998 9881967 denotes 18
18162042-16467870-84705017 14406-14407 16467870 denotes 3
18162042-16484453-84705018 15462-15464 16484453 denotes 19
18162042-11483607-84705019 19570-19572 11483607 denotes 20
18162042-9846495-84705020 20601-20602 9846495 denotes 2
18162042-12648458-84705021 20603-20605 12648458 denotes 21
18162042-11441076-84705022 21769-21771 11441076 denotes 22
18162042-9335507-84705023 22356-22358 9335507 denotes 23
18162042-16517728-84705024 23760-23762 16517728 denotes 24
18162042-16517728-84705025 23984-23986 16517728 denotes 24
18162042-8083467-84705026 24141-24143 8083467 denotes 25
18162042-7654359-84705027 24144-24146 7654359 denotes 26
18162042-14597769-84705028 27996-27998 14597769 denotes 12
18162042-16617117-84705029 27999-28001 16617117 denotes 27
18162042-14676299-84705030 28311-28313 14676299 denotes 10
18162042-15100250-84705031 28314-28316 15100250 denotes 28
18162042-15659261-84705031 28314-28316 15659261 denotes 28
18162042-15232609-84705031 28314-28316 15232609 denotes 28
18162042-16244650-84705031 28314-28316 16244650 denotes 28
18162042-15237111-84705031 28314-28316 15237111 denotes 28
18162042-15795373-84705031 28314-28316 15795373 denotes 28
18162042-17298177-84705031 28314-28316 17298177 denotes 28
18162042-10761931-84705032 28575-28577 10761931 denotes 35
18162042-9160750-84705033 28578-28580 9160750 denotes 36
18162042-11045997-84705034 28901-28903 11045997 denotes 37
18162042-11009098-84705035 28904-28906 11009098 denotes 38
18162042-15577848-84705036 29291-29293 15577848 denotes 39
18162042-16517699-84705037 29304-29306 16517699 denotes 40
18162042-15294932-84705038 29324-29326 15294932 denotes 41
18162042-12524538-84705039 29379-29381 12524538 denotes 42
18162042-16237062-84705039 29379-29381 16237062 denotes 42
18162042-16476055-84705039 29379-29381 16476055 denotes 42
18162042-11544451-84705039 29379-29381 11544451 denotes 42
18162042-16484453-84705040 29888-29890 16484453 denotes 19
18162042-16200070-84705041 30591-30593 16200070 denotes 46
18162042-15944265-84705042 30594-30596 15944265 denotes 47
18162042-16473830-84705043 30794-30796 16473830 denotes 48
18162042-16648838-84705044 30797-30799 16648838 denotes 49
18162042-16237107-84705045 31001-31003 16237107 denotes 50
18162042-15944265-84705046 31284-31286 15944265 denotes 47
18162042-16645171-84705047 31355-31357 16645171 denotes 51
18162042-16121185-84705048 32797-32799 16121185 denotes 52
18162042-12871641-84705049 33056-33058 12871641 denotes 53
18162042-14769923-84705050 33059-33061 14769923 denotes 54
18162042-16943277-84705051 33168-33170 16943277 denotes 55
18162042-16517728-84705052 33521-33523 16517728 denotes 24
18162042-10880531-84705053 34050-34052 10880531 denotes 56
18162042-9160750-84705054 34094-34096 9160750 denotes 36
18162042-12648458-84705055 34174-34176 12648458 denotes 21
18162042-9801155-84705056 34177-34179 9801155 denotes 57
18162042-11579103-84705056 34177-34179 11579103 denotes 57
18162042-17075044-84705056 34177-34179 17075044 denotes 57
18162042-16517728-84705057 34481-34483 16517728 denotes 24
18162042-11441076-84705058 35463-35465 11441076 denotes 22
18162042-12554698-84705059 35960-35962 12554698 denotes 60
18162042-16387593-84705060 36553-36555 16387593 denotes 61
18162042-10037236-84705061 38031-38033 10037236 denotes 62
18162042-16517728-84705062 39043-39045 16517728 denotes 24
18162042-17298430-84705063 40495-40497 17298430 denotes 63
T91765 3614-3615 10626890 denotes 1
T15814 3616-3617 9846495 denotes 2
T97197 3667-3668 16467870 denotes 3
T83455 3816-3817 16467870 denotes 3
T26868 4069-4070 16520391 denotes 4
T53446 4071-4072 15662016 denotes 5
T76890 4170-4171 16489117 denotes 6
T96105 4247-4248 15634907 denotes 7
T85669 4304-4305 10549629 denotes 8
T87774 4360-4361 11390432 denotes 9
T61485 5113-5115 14676299 denotes 10
T25969 5283-5285 15753318 denotes 11
T96747 5286-5288 14597769 denotes 12
T99026 5348-5350 17185041 denotes 13
T47692 5351-5353 17154262 denotes 14
T1813 5549-5551 15809351 denotes 15
T36804 5621-5623 11045997 denotes 16
T37257 5734-5736 15809351 denotes 15
T43404 5737-5739 16973386 denotes 17
T17616 11996-11998 9881967 denotes 18
T69733 14406-14407 16467870 denotes 3
T89214 15462-15464 16484453 denotes 19
T41406 19570-19572 11483607 denotes 20
T32923 20601-20602 9846495 denotes 2
T97991 20603-20605 12648458 denotes 21
T7965 21769-21771 11441076 denotes 22
T9118 22356-22358 9335507 denotes 23
T17011 23760-23762 16517728 denotes 24
T99449 23984-23986 16517728 denotes 24
T74593 24141-24143 8083467 denotes 25
T43549 24144-24146 7654359 denotes 26
T86367 27996-27998 14597769 denotes 12
T42145 27999-28001 16617117 denotes 27
T19301 28311-28313 14676299 denotes 10
T33501 28314-28316 15100250 denotes 28
T60240 28314-28316 15659261 denotes 28
T94836 28314-28316 15232609 denotes 28
T79942 28314-28316 16244650 denotes 28
T22720 28314-28316 15237111 denotes 28
T18269 28314-28316 15795373 denotes 28
T10908 28314-28316 17298177 denotes 28
T74203 28575-28577 10761931 denotes 35
T72895 28578-28580 9160750 denotes 36
T39348 28901-28903 11045997 denotes 37
T26194 28904-28906 11009098 denotes 38
T18872 29291-29293 15577848 denotes 39
T75575 29304-29306 16517699 denotes 40
T60830 29324-29326 15294932 denotes 41
T52569 29379-29381 12524538 denotes 42
T47052 29379-29381 16237062 denotes 42
T12050 29379-29381 16476055 denotes 42
T38379 29379-29381 11544451 denotes 42
T23630 29888-29890 16484453 denotes 19
T81207 30591-30593 16200070 denotes 46
T96838 30594-30596 15944265 denotes 47
T27391 30794-30796 16473830 denotes 48
T74470 30797-30799 16648838 denotes 49
T49545 31001-31003 16237107 denotes 50
T48283 31284-31286 15944265 denotes 47
T69471 31355-31357 16645171 denotes 51
T40204 32797-32799 16121185 denotes 52
T35393 33056-33058 12871641 denotes 53
T74415 33059-33061 14769923 denotes 54
T84125 33168-33170 16943277 denotes 55
T5605 33521-33523 16517728 denotes 24
T14624 34050-34052 10880531 denotes 56
T18087 34094-34096 9160750 denotes 36
T24855 34174-34176 12648458 denotes 21
T68755 34177-34179 9801155 denotes 57
T99096 34177-34179 11579103 denotes 57
T56091 34177-34179 17075044 denotes 57
T4867 34481-34483 16517728 denotes 24
T68613 35463-35465 11441076 denotes 22
T44281 35960-35962 12554698 denotes 60
T86850 36553-36555 16387593 denotes 61
T11976 38031-38033 10037236 denotes 62
T56825 39043-39045 16517728 denotes 24
T99961 40495-40497 17298430 denotes 63

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T107 0-12 JJ denotes GATA3-Driven
T108 13-16 NN denotes Th2
T109 17-26 NN denotes Responses
T110 27-34 VB denotes Inhibit
T111 35-49 JJ denotes TGF-β1–Induced
T112 50-55 NN denotes FOXP3
T113 56-66 NN denotes Expression
T114 67-70 CC denotes and
T115 71-74 DT denotes the
T116 75-84 NN denotes Formation
T117 85-87 IN denotes of
T118 88-98 JJ denotes Regulatory
T119 99-100 NN denotes T
T120 101-106 NN denotes Cells
T121 159-172 NN denotes Transcription
T122 173-180 NN denotes factors
T123 181-184 VB denotes act
T124 185-187 IN denotes in
T125 188-195 NN denotes concert
T126 196-198 TO denotes to
T127 199-205 VB denotes induce
T128 206-213 NN denotes lineage
T129 214-224 NN denotes commitment
T130 225-232 IN denotes towards
T131 233-236 NN denotes Th1
T132 236-237 -COMMA- denotes ,
T133 238-241 NN denotes Th2
T134 241-242 -COMMA- denotes ,
T135 243-245 CC denotes or
T136 246-247 NN denotes T
T137 248-258 JJ denotes regulatory
T138 259-260 -LRB- denotes (
T139 260-264 NN denotes Treg
T140 264-265 -RRB- denotes )
T141 266-271 NN denotes cells
T142 271-272 -COMMA- denotes ,
T143 273-276 CC denotes and
T144 277-282 PRP-DOLLAR- denotes their
T145 283-301 JJ denotes counter-regulatory
T146 302-312 NN denotes mechanisms
T147 313-317 VB denotes were
T148 318-323 VB denotes shown
T149 324-326 TO denotes to
T150 327-329 VB denotes be
T151 330-338 JJ denotes critical
T152 339-342 IN denotes for
T153 343-355 NN denotes polarization
T154 356-363 IN denotes between
T155 364-367 NN denotes Th1
T156 368-371 CC denotes and
T157 372-375 NN denotes Th2
T158 376-386 NN denotes phenotypes
T159 388-393 NN denotes FOXP3
T160 394-396 VB denotes is
T161 397-399 DT denotes an
T162 400-409 JJ denotes essential
T163 410-423 NN denotes transcription
T164 424-430 NN denotes factor
T165 431-434 IN denotes for
T166 435-442 JJ denotes natural
T167 442-443 -COMMA- denotes ,
T168 444-458 JJ denotes thymus-derived
T169 459-460 -LRB- denotes (
T170 460-465 NN denotes nTreg
T171 465-466 -RRB- denotes )
T172 467-470 CC denotes and
T173 471-480 JJ denotes inducible
T174 481-485 NN denotes Treg
T175 486-487 -LRB- denotes (
T176 487-492 NN denotes iTreg
T177 492-493 -RRB- denotes )
T178 494-504 NN denotes commitment
T179 504-505 -COLON- denotes ;
T180 506-513 RB denotes however
T181 513-514 -COMMA- denotes ,
T182 515-518 DT denotes the
T183 519-529 NN denotes mechanisms
T184 530-540 VB denotes regulating
T185 541-544 PRP-DOLLAR- denotes its
T186 545-555 NN denotes expression
T187 556-559 VB denotes are
T188 560-562 RB denotes as
T189 563-566 RB denotes yet
T190 567-574 JJ denotes unknown
T191 576-578 PRP denotes We
T192 579-587 VB denotes describe
T193 588-589 DT denotes a
T194 590-599 NN denotes mechanism
T195 600-611 VB denotes controlling
T196 612-617 NN denotes iTreg
T197 618-630 NN denotes polarization
T198 630-631 -COMMA- denotes ,
T199 632-637 WDT denotes which
T200 638-640 VB denotes is
T201 641-650 VB denotes overruled
T202 651-653 IN denotes by
T203 654-657 DT denotes the
T204 658-661 NN denotes Th2
T205 662-677 NN denotes differentiation
T206 678-685 NN denotes pathway
T207 687-689 PRP denotes We
T208 690-702 VB denotes demonstrated
T209 703-707 IN denotes that
T210 708-719 NN denotes interleukin
T211 720-721 CD denotes 4
T212 722-723 -LRB- denotes (
T213 723-727 NN denotes IL-4
T214 727-728 -RRB- denotes )
T215 729-736 JJ denotes present
T216 737-739 IN denotes at
T217 740-743 DT denotes the
T218 744-748 NN denotes time
T219 749-751 IN denotes of
T220 752-753 NN denotes T
T221 754-758 NN denotes cell
T222 759-766 NN denotes priming
T223 767-775 VB denotes inhibits
T224 776-781 NN denotes FOXP3
T225 783-787 DT denotes This
T226 788-798 JJ denotes inhibitory
T227 799-808 NN denotes mechanism
T228 809-812 VB denotes was
T229 813-817 RB denotes also
T230 818-827 VB denotes confirmed
T231 828-830 IN denotes in
T232 831-834 NN denotes Th2
T233 835-840 NN denotes cells
T234 841-844 CC denotes and
T235 845-847 IN denotes in
T236 848-849 NN denotes T
T237 850-855 NN denotes cells
T238 856-858 IN denotes of
T239 859-869 JJ denotes transgenic
T240 870-874 NN denotes mice
T241 875-889 VB denotes overexpressing
T242 890-896 NN denotes GATA-3
T243 897-899 IN denotes in
T244 900-901 NN denotes T
T245 902-907 NN denotes cells
T246 907-908 -COMMA- denotes ,
T247 909-914 WDT denotes which
T248 915-918 VB denotes are
T249 919-924 VB denotes shown
T250 925-927 TO denotes to
T251 928-930 VB denotes be
T252 931-940 JJ denotes deficient
T253 941-943 IN denotes in
T254 944-956 VB denotes transforming
T255 957-963 NN denotes growth
T256 964-970 NN denotes factor
T257 971-972 -LRB- denotes (
T258 972-975 NN denotes TGF
T259 975-976 -RRB- denotes )
T260 976-987 JJ denotes -β–mediated
T261 988-993 NN denotes FOXP3
T262 994-1003 NN denotes induction
T263 1005-1009 DT denotes This
T264 1010-1020 NN denotes inhibition
T265 1021-1023 VB denotes is
T266 1024-1032 VB denotes mediated
T267 1033-1035 IN denotes by
T268 1036-1042 JJ denotes direct
T269 1043-1050 NN denotes binding
T270 1051-1053 IN denotes of
T271 1054-1059 NN denotes GATA3
T272 1060-1062 TO denotes to
T273 1063-1066 DT denotes the
T274 1067-1072 NN denotes FOXP3
T275 1073-1081 NN denotes promoter
T276 1081-1082 -COMMA- denotes ,
T277 1083-1088 WDT denotes which
T278 1089-1098 VB denotes represses
T279 1099-1102 PRP-DOLLAR- denotes its
T280 1103-1118 NN denotes transactivation
T281 1119-1126 NN denotes process
T282 1128-1137 RB denotes Therefore
T283 1137-1138 -COMMA- denotes ,
T284 1139-1143 DT denotes this
T285 1144-1149 NN denotes study
T286 1150-1158 VB denotes provides
T287 1159-1160 DT denotes a
T288 1161-1164 JJ denotes new
T289 1165-1178 NN denotes understanding
T290 1179-1181 IN denotes of
T291 1182-1191 NN denotes tolerance
T292 1192-1203 NN denotes development
T293 1203-1204 -COMMA- denotes ,
T294 1205-1215 VB denotes controlled
T295 1216-1218 IN denotes by
T296 1219-1220 DT denotes a
T297 1221-1225 NN denotes type
T298 1226-1227 CD denotes 2
T299 1228-1234 JJ denotes immune
T300 1235-1243 NN denotes response
T301 1245-1249 NN denotes IL-4
T302 1250-1259 NN denotes treatment
T303 1260-1262 IN denotes in
T304 1263-1267 NN denotes mice
T305 1268-1275 VB denotes reduces
T306 1276-1281 NN denotes iTreg
T307 1282-1286 NN denotes cell
T308 1287-1296 NN denotes frequency
T309 1296-1297 -COMMA- denotes ,
T310 1298-1310 VB denotes highlighting
T311 1311-1315 IN denotes that
T312 1316-1327 JJ denotes therapeutic
T313 1328-1338 NN denotes approaches
T314 1339-1343 WDT denotes that
T315 1344-1350 VB denotes target
T316 1351-1355 NN denotes IL-4
T317 1356-1358 CC denotes or
T318 1359-1364 NN denotes GATA3
T319 1365-1370 MD denotes might
T320 1371-1378 VB denotes provide
T321 1379-1382 JJ denotes new
T322 1383-1393 JJ denotes preventive
T323 1394-1404 NN denotes strategies
T324 1405-1417 VB denotes facilitating
T325 1418-1427 NN denotes tolerance
T326 1428-1437 NN denotes induction
T327 1438-1450 RB denotes particularly
T328 1451-1453 IN denotes in
T329 1454-1466 JJ denotes Th2-mediated
T330 1467-1475 NN denotes diseases
T331 1475-1476 -COMMA- denotes ,
T332 1477-1481 JJ denotes such
T333 1482-1484 IN denotes as
T334 1485-1492 NN denotes allergy
T1011 2849-2858 JJ denotes Effective
T1012 2859-2865 JJ denotes immune
T1013 2866-2875 NN denotes responses
T1014 2876-2879 VB denotes are
T1015 2880-2893 VB denotes characterized
T1016 2894-2896 IN denotes by
T1017 2897-2898 NN denotes T
T1018 2899-2903 NN denotes cell
T1019 2904-2914 NN denotes activation
T1020 2914-2915 -COMMA- denotes ,
T1021 2916-2921 WDT denotes which
T1022 2922-2929 VB denotes directs
T1023 2930-2938 JJ denotes adaptive
T1024 2939-2942 CC denotes and
T1025 2943-2949 JJ denotes innate
T1026 2950-2956 JJ denotes immune
T1027 2957-2966 NN denotes responses
T1028 2967-2969 TO denotes to
T1029 2970-2974 VB denotes kill
T1030 2975-2984 NN denotes pathogens
T1031 2985-2996 RB denotes efficiently
T1032 2998-3007 JJ denotes Dependent
T1033 3008-3010 IN denotes on
T1034 3011-3014 DT denotes the
T1035 3015-3023 NN denotes pathogen
T1036 3023-3024 -COMMA- denotes ,
T1037 3025-3026 NN denotes T
T1038 3027-3032 NN denotes cells
T1039 3033-3046 VB denotes differentiate
T1040 3047-3051 IN denotes into
T1041 3052-3061 JJ denotes different
T1042 3062-3070 NN denotes subtypes
T1043 3070-3071 -COMMA- denotes ,
T1044 3072-3076 JJ denotes such
T1045 3077-3079 IN denotes as
T1046 3080-3083 NN denotes Th1
T1047 3084-3086 CC denotes or
T1048 3087-3090 NN denotes Th2
T1049 3091-3096 NN denotes cells
T1050 3096-3097 -COMMA- denotes ,
T1051 3098-3103 WDT denotes which
T1052 3104-3107 VB denotes are
T1053 3108-3112 RB denotes most
T1054 3113-3122 JJ denotes efficient
T1055 3123-3125 IN denotes in
T1056 3126-3135 VB denotes defeating
T1057 3136-3145 JJ denotes microbial
T1058 3146-3148 CC denotes or
T1059 3149-3158 JJ denotes parasitic
T1060 3159-3167 NN denotes invaders
T1061 3168-3180 RB denotes respectively
T1062 3182-3183 DT denotes A
T1063 3184-3192 NN denotes hallmark
T1064 3193-3195 IN denotes of
T1065 3196-3199 NN denotes Th1
T1066 3200-3203 CC denotes and
T1067 3204-3207 NN denotes Th2
T1068 3208-3223 NN denotes differentiation
T1069 3224-3232 NN denotes pathways
T1070 3233-3235 VB denotes is
T1071 3236-3239 DT denotes the
T1072 3240-3253 NN denotes exclusiveness
T1073 3254-3256 IN denotes of
T1074 3257-3260 DT denotes the
T1075 3261-3271 JJ denotes individual
T1076 3272-3281 NN denotes phenotype
T1077 3282-3289 VB denotes leading
T1078 3290-3292 TO denotes to
T1079 3293-3299 CC denotes either
T1080 3300-3303 NN denotes Th1
T1081 3304-3306 CC denotes or
T1082 3307-3310 NN denotes Th2
T1083 3310-3311 -COMMA- denotes ,
T1084 3312-3315 CC denotes but
T1085 3316-3319 RB denotes not
T1086 3320-3322 TO denotes to
T1087 3323-3328 JJ denotes mixed
T1088 3329-3340 NN denotes populations
T1089 3342-3345 DT denotes The
T1090 3346-3359 NN denotes exclusiveness
T1091 3360-3362 IN denotes of
T1092 3363-3367 DT denotes this
T1093 3368-3377 NN denotes mechanism
T1094 3378-3380 VB denotes is
T1095 3381-3389 VB denotes provided
T1096 3390-3392 IN denotes by
T1097 3393-3394 DT denotes a
T1098 3395-3407 NN denotes polarization
T1099 3408-3415 NN denotes process
T1100 3415-3416 -COMMA- denotes ,
T1101 3417-3422 WRB denotes where
T1102 3423-3426 NN denotes Th2
T1103 3427-3442 NN denotes differentiation
T1104 3443-3451 VB denotes inhibits
T1105 3452-3455 NN denotes Th1
T1106 3456-3466 NN denotes commitment
T1107 3467-3470 CC denotes and
T1108 3471-3475 NN denotes vice
T1109 3476-3481 NN denotes versa
T1110 3483-3495 RB denotes Specifically
T1111 3496-3507 NN denotes interleukin
T1112 3508-3509 CD denotes 4
T1113 3510-3511 -LRB- denotes (
T1114 3511-3515 NN denotes IL-4
T1115 3515-3516 -RRB- denotes )
T1116 3516-3524 VB denotes –induced
T1117 3525-3530 NN denotes STAT6
T1118 3531-3534 CC denotes and
T1119 3535-3540 NN denotes GATA3
T1120 3541-3548 VB denotes inhibit
T1121 3549-3564 NN denotes differentiation
T1122 3565-3569 IN denotes into
T1123 3570-3573 NN denotes Th1
T1124 3574-3579 NN denotes cells
T1125 3580-3582 IN denotes in
T1126 3583-3586 DT denotes the
T1127 3587-3592 JJ denotes early
T1128 3593-3598 NN denotes phase
T1129 3599-3601 IN denotes of
T1130 3602-3612 NN denotes commitment
T1131 3613-3614 -LRB- denotes [
T1132 3614-3617 CD denotes 1,2
T1133 3617-3618 -RRB- denotes ]
T1134 3620-3625 NN denotes GATA3
T1135 3626-3628 VB denotes is
T1136 3629-3639 JJ denotes sufficient
T1137 3640-3642 TO denotes to
T1138 3643-3649 VB denotes induce
T1139 3650-3651 DT denotes a
T1140 3652-3655 NN denotes Th2
T1141 3656-3665 NN denotes phenotype
T1142 3666-3667 -LRB- denotes [
T1143 3667-3668 CD denotes 3
T1144 3668-3669 -RRB- denotes ]
T1145 3670-3673 CC denotes and
T1146 3674-3678 VB denotes acts
T1147 3679-3682 RB denotes not
T1148 3683-3687 RB denotes only
T1149 3688-3695 IN denotes through
T1150 3696-3699 DT denotes the
T1151 3700-3709 NN denotes induction
T1152 3710-3712 IN denotes of
T1153 3713-3717 NN denotes IL-4
T1154 3717-3718 -COMMA- denotes ,
T1155 3719-3723 NN denotes IL-5
T1156 3724-3727 CC denotes and
T1157 3728-3733 NN denotes IL-13
T1158 3733-3734 -COMMA- denotes ,
T1159 3735-3738 DT denotes the
T1160 3739-3742 NN denotes Th2
T1161 3743-3752 NN denotes cytokines
T1162 3752-3753 -COMMA- denotes ,
T1163 3754-3757 CC denotes but
T1164 3758-3762 RB denotes also
T1165 3763-3770 IN denotes through
T1166 3771-3774 DT denotes the
T1167 3775-3785 NN denotes inhibition
T1168 3786-3788 IN denotes of
T1169 3789-3792 NN denotes Th1
T1170 3793-3806 JJ denotes cell-specific
T1171 3807-3814 NN denotes factors
T1172 3815-3816 -LRB- denotes [
T1173 3816-3817 CD denotes 3
T1174 3817-3818 -RRB- denotes ]
T1175 3820-3828 RB denotes Recently
T1176 3828-3829 -COMMA- denotes ,
T1177 3830-3832 PRP denotes it
T1178 3833-3836 VB denotes was
T1179 3837-3842 VB denotes shown
T1180 3843-3847 IN denotes that
T1181 3848-3853 NN denotes T-bet
T1182 3854-3862 RB denotes directly
T1183 3863-3872 VB denotes modulates
T1184 3873-3878 NN denotes GATA3
T1185 3879-3887 NN denotes function
T1186 3887-3888 -COMMA- denotes ,
T1187 3889-3899 VB denotes suggesting
T1188 3900-3904 IN denotes that
T1189 3905-3918 NN denotes transcription
T1190 3919-3926 NN denotes factors
T1191 3927-3934 VB denotes compete
T1192 3935-3937 IN denotes in
T1193 3938-3941 DT denotes the
T1194 3942-3947 JJ denotes early
T1195 3948-3963 NN denotes differentiation
T1196 3964-3969 NN denotes phase
T1197 3970-3972 IN denotes of
T1198 3973-3974 NN denotes T
T1199 3975-3980 NN denotes cells
T1200 3980-3981 -COMMA- denotes ,
T1201 3982-3993 RB denotes potentially
T1202 3994-4005 VB denotes integrating
T1203 4006-4019 JJ denotes environmental
T1204 4020-4027 NN denotes signals
T1205 4028-4030 TO denotes to
T1206 4031-4038 RB denotes finally
T1207 4039-4046 VB denotes imprint
T1208 4047-4050 DT denotes the
T1209 4051-4052 NN denotes T
T1210 4053-4057 NN denotes cell
T1211 4058-4067 NN denotes phenotype
T1212 4068-4069 -LRB- denotes [
T1213 4069-4072 CD denotes 4,5
T1214 4072-4073 -RRB- denotes ]
T1215 4075-4076 DT denotes A
T1216 4077-4092 JJ denotes GATA3-dominated
T1217 4093-4099 JJ denotes immune
T1218 4100-4108 NN denotes response
T1219 4109-4112 VB denotes has
T1220 4113-4117 VB denotes been
T1221 4118-4123 VB denotes shown
T1222 4124-4126 TO denotes to
T1223 4127-4129 VB denotes be
T1224 4130-4139 JJ denotes essential
T1225 4140-4142 IN denotes in
T1226 4143-4149 NN denotes airway
T1227 4150-4168 NN denotes hyperresonsiveness
T1228 4169-4170 -LRB- denotes [
T1229 4170-4171 CD denotes 6
T1230 4171-4172 -RRB- denotes ]
T1231 4173-4176 CC denotes and
T1232 4177-4191 JJ denotes IL-4–dominated
T1233 4192-4201 NN denotes responses
T1234 4202-4205 MD denotes can
T1235 4206-4211 VB denotes break
T1236 4212-4228 JJ denotes antigen-specific
T1237 4229-4235 JJ denotes immune
T1238 4236-4245 NN denotes tolerance
T1239 4246-4247 -LRB- denotes [
T1240 4247-4248 CD denotes 7
T1241 4248-4249 -RRB- denotes ]
T1242 4251-4265 NN denotes Overexpression
T1243 4266-4268 IN denotes of
T1244 4269-4270 DT denotes a
T1245 4271-4279 JJ denotes dominant
T1246 4280-4288 JJ denotes negative
T1247 4289-4293 NN denotes form
T1248 4294-4296 IN denotes of
T1249 4297-4302 NN denotes GATA3
T1250 4303-4304 -LRB- denotes [
T1251 4304-4305 CD denotes 8
T1252 4305-4306 -RRB- denotes ]
T1253 4307-4309 CC denotes or
T1254 4310-4319 NN denotes treatment
T1255 4320-4324 IN denotes with
T1256 4325-4343 JJ denotes antisense-mediated
T1257 4344-4349 NN denotes GATA3
T1258 4350-4358 NN denotes blockade
T1259 4359-4360 -LRB- denotes [
T1260 4360-4361 CD denotes 9
T1261 4361-4362 -RRB- denotes ]
T1262 4363-4372 VB denotes decreased
T1263 4373-4376 DT denotes the
T1264 4377-4385 NN denotes severity
T1265 4386-4388 IN denotes of
T1266 4389-4392 DT denotes the
T1267 4393-4401 JJ denotes allergic
T1268 4402-4408 NN denotes airway
T1269 4409-4429 NN denotes hyper-responsiveness
T1270 4431-4434 DT denotes The
T1271 4435-4444 NN denotes discovery
T1272 4445-4447 IN denotes of
T1273 4448-4458 JJ denotes regulatory
T1274 4459-4460 NN denotes T
T1275 4461-4462 -LRB- denotes (
T1276 4462-4466 NN denotes Treg
T1277 4466-4467 -RRB- denotes )
T1278 4468-4473 NN denotes cells
T1279 4474-4484 VB denotes highlights
T1280 4485-4492 DT denotes another
T1281 4493-4502 NN denotes phenotype
T1282 4503-4505 IN denotes of
T1283 4506-4507 NN denotes T
T1284 4508-4513 NN denotes cells
T1285 4513-4514 -COMMA- denotes ,
T1286 4515-4520 WDT denotes which
T1287 4521-4523 VB denotes is
T1288 4524-4533 JJ denotes essential
T1289 4534-4537 IN denotes for
T1290 4538-4547 NN denotes tolerance
T1291 4548-4555 IN denotes against
T1292 4556-4569 NN denotes self-antigens
T1293 4571-4580 RB denotes Naturally
T1294 4581-4590 VB denotes occurring
T1295 4590-4591 -COMMA- denotes ,
T1296 4592-4606 JJ denotes thymus-derived
T1297 4607-4611 NN denotes Treg
T1298 4612-4613 -LRB- denotes (
T1299 4613-4618 NN denotes nTreg
T1300 4618-4619 -RRB- denotes )
T1301 4620-4625 NN denotes cells
T1302 4626-4629 VB denotes are
T1303 4630-4639 VB denotes generated
T1304 4640-4642 IN denotes in
T1305 4643-4646 DT denotes the
T1306 4647-4653 NN denotes thymus
T1307 4654-4657 CC denotes and
T1308 4658-4661 VB denotes are
T1309 4662-4669 VB denotes assumed
T1310 4670-4672 TO denotes to
T1311 4673-4680 VB denotes protect
T1312 4681-4688 IN denotes against
T1313 4689-4692 DT denotes the
T1314 4693-4701 NN denotes activity
T1315 4702-4704 IN denotes of
T1316 4705-4717 JJ denotes autoreactive
T1317 4718-4719 NN denotes T
T1318 4720-4725 NN denotes cells
T1319 4726-4728 IN denotes in
T1320 4729-4732 DT denotes the
T1321 4733-4742 NN denotes periphery
T1322 4744-4749 DT denotes These
T1323 4750-4755 NN denotes cells
T1324 4756-4763 VB denotes express
T1325 4764-4767 DT denotes the
T1326 4768-4776 NN denotes forkhead
T1327 4777-4790 NN denotes transcription
T1328 4791-4797 NN denotes factor
T1329 4798-4803 NN denotes FOXP3
T1330 4804-4807 CC denotes and
T1331 4808-4822 RB denotes constitutively
T1332 4823-4830 VB denotes express
T1333 4831-4835 NN denotes CD25
T1334 4836-4838 IN denotes on
T1335 4839-4844 PRP-DOLLAR- denotes their
T1336 4845-4852 NN denotes surface
T1337 4852-4853 -COMMA- denotes ,
T1338 4854-4857 CC denotes but
T1339 4858-4862 PRP denotes they
T1340 4863-4867 VB denotes lack
T1341 4868-4878 NN denotes expression
T1342 4879-4881 IN denotes of
T1343 4882-4885 NN denotes Th1
T1344 4886-4888 CC denotes or
T1345 4889-4892 NN denotes Th2
T1346 4893-4902 NN denotes cytokines
T1347 4904-4916 RB denotes Particularly
T1348 4917-4928 JJ denotes interesting
T1349 4929-4932 VB denotes are
T1350 4933-4938 DT denotes those
T1351 4939-4943 NN denotes Treg
T1352 4944-4949 NN denotes cells
T1353 4950-4954 WDT denotes that
T1354 4955-4958 VB denotes are
T1355 4959-4968 VB denotes generated
T1356 4969-4971 IN denotes in
T1357 4972-4975 DT denotes the
T1358 4976-4985 NN denotes periphery
T1359 4986-4989 CC denotes and
T1360 4990-4994 RB denotes thus
T1361 4995-4998 VB denotes are
T1362 4999-5008 JJ denotes potential
T1363 5009-5016 NN denotes targets
T1364 5017-5020 IN denotes for
T1365 5021-5032 JJ denotes therapeutic
T1366 5033-5046 NN denotes interventions
T1367 5048-5053 DT denotes These
T1368 5054-5061 VB denotes induced
T1369 5062-5066 NN denotes Treg
T1370 5067-5068 -LRB- denotes (
T1371 5068-5073 NN denotes iTreg
T1372 5073-5074 -RRB- denotes )
T1373 5075-5080 NN denotes cells
T1374 5081-5085 VB denotes were
T1375 5086-5094 VB denotes reported
T1376 5095-5097 TO denotes to
T1377 5098-5105 VB denotes express
T1378 5106-5111 NN denotes FOXP3
T1379 5112-5113 -LRB- denotes [
T1380 5113-5115 CD denotes 10
T1381 5115-5116 -RRB- denotes ]
T1382 5118-5121 DT denotes The
T1383 5122-5127 JJ denotes exact
T1384 5128-5138 NN denotes mechanisms
T1385 5139-5141 IN denotes of
T1386 5142-5147 NN denotes iTreg
T1387 5148-5158 NN denotes generation
T1388 5159-5162 VB denotes are
T1389 5163-5170 JJ denotes unclear
T1390 5170-5171 -COMMA- denotes ,
T1391 5172-5175 CC denotes but
T1392 5176-5177 NN denotes T
T1393 5178-5182 NN denotes cell
T1394 5183-5191 NN denotes receptor
T1395 5192-5193 -LRB- denotes (
T1396 5193-5196 NN denotes TCR
T1397 5196-5197 -RRB- denotes )
T1398 5198-5208 NN denotes triggering
T1399 5209-5212 VB denotes has
T1400 5213-5217 VB denotes been
T1401 5218-5223 VB denotes shown
T1402 5224-5226 TO denotes to
T1403 5227-5233 VB denotes induce
T1404 5234-5239 NN denotes FOXP3
T1405 5240-5250 NN denotes expression
T1406 5251-5254 CC denotes and
T1407 5255-5266 JJ denotes suppressive
T1408 5267-5272 NN denotes cells
T1409 5273-5275 IN denotes in
T1410 5276-5281 JJ denotes human
T1411 5282-5283 -LRB- denotes [
T1412 5283-5288 CD denotes 11,12
T1413 5288-5289 -RRB- denotes ]
T1414 5289-5290 -COMMA- denotes ,
T1415 5291-5298 RB denotes however
T1416 5299-5302 DT denotes the
T1417 5303-5312 NN denotes phenotype
T1418 5313-5320 VB denotes appears
T1419 5321-5323 TO denotes to
T1420 5324-5326 VB denotes be
T1421 5327-5329 IN denotes of
T1422 5330-5339 JJ denotes transient
T1423 5340-5346 NN denotes nature
T1424 5347-5348 -LRB- denotes [
T1425 5348-5353 CD denotes 13,14
T1426 5353-5354 -RRB- denotes ]
T1427 5356-5361 NN denotes TGF-β
T1428 5362-5365 VB denotes has
T1429 5366-5370 VB denotes been
T1430 5371-5383 VB denotes demonstrated
T1431 5384-5386 TO denotes to
T1432 5387-5389 VB denotes be
T1433 5390-5399 JJ denotes important
T1434 5400-5403 IN denotes for
T1435 5404-5407 DT denotes the
T1436 5408-5418 JJ denotes persistent
T1437 5419-5428 NN denotes induction
T1438 5429-5431 IN denotes of
T1439 5432-5437 DT denotes these
T1440 5438-5443 NN denotes cells
T1441 5444-5446 FW denotes in
T1442 5447-5452 FW denotes vitro
T1443 5453-5456 CC denotes and
T1444 5457-5459 FW denotes in
T1445 5460-5464 FW denotes vivo
T1446 5464-5465 -COMMA- denotes ,
T1447 5466-5471 IN denotes since
T1448 5472-5479 NN denotes animals
T1449 5480-5487 VB denotes lacking
T1450 5488-5491 DT denotes the
T1451 5492-5500 NN denotes TGF-βRII
T1452 5501-5503 IN denotes on
T1453 5504-5505 NN denotes T
T1454 5506-5511 NN denotes cells
T1455 5512-5516 VB denotes have
T1456 5517-5522 JJ denotes fewer
T1457 5523-5535 RB denotes peripherally
T1458 5536-5541 NN denotes iTreg
T1459 5542-5547 NN denotes cells
T1460 5548-5549 -LRB- denotes [
T1461 5549-5551 CD denotes 15
T1462 5551-5552 -RRB- denotes ]
T1463 5553-5556 CC denotes and
T1464 5557-5563 VB denotes suffer
T1465 5564-5568 IN denotes from
T1466 5569-5570 DT denotes a
T1467 5571-5572 NN denotes T
T1468 5573-5587 JJ denotes cell–dependent
T1469 5588-5598 JJ denotes multiorgan
T1470 5599-5611 JJ denotes inflammatory
T1471 5612-5619 NN denotes disease
T1472 5620-5621 -LRB- denotes [
T1473 5621-5623 CD denotes 16
T1474 5623-5624 -RRB- denotes ]
T1475 5626-5634 IN denotes Although
T1476 5635-5638 DT denotes the
T1477 5639-5645 NN denotes effect
T1478 5646-5648 IN denotes of
T1479 5649-5654 NN denotes TGF-β
T1480 5655-5657 IN denotes on
T1481 5658-5665 JJ denotes natural
T1482 5666-5669 CC denotes and
T1483 5670-5679 JJ denotes inducible
T1484 5680-5684 NN denotes Treg
T1485 5685-5689 NN denotes cell
T1486 5690-5699 NN denotes induction
T1487 5700-5703 VB denotes has
T1488 5704-5708 VB denotes been
T1489 5709-5721 VB denotes demonstrated
T1490 5722-5732 RB denotes repeatedly
T1491 5733-5734 -LRB- denotes [
T1492 5734-5739 CD denotes 15,17
T1493 5739-5740 -RRB- denotes ]
T1494 5740-5741 -COMMA- denotes ,
T1495 5742-5745 PRP-DOLLAR- denotes its
T1496 5746-5755 JJ denotes molecular
T1497 5756-5766 NN denotes mechanisms
T1498 5767-5773 VB denotes remain
T1499 5774-5776 TO denotes to
T1500 5777-5779 VB denotes be
T1501 5780-5790 VB denotes identified
T1502 5792-5795 DT denotes The
T1503 5796-5803 JJ denotes current
T1504 5804-5809 NN denotes study
T1505 5810-5818 VB denotes provides
T1506 5819-5827 NN denotes evidence
T1507 5828-5832 IN denotes that
T1508 5833-5838 NN denotes GATA3
T1509 5839-5842 CC denotes and
T1510 5843-5848 NN denotes FOXP3
T1511 5849-5853 VB denotes play
T1512 5854-5855 DT denotes a
T1513 5856-5867 JJ denotes competitive
T1514 5868-5872 NN denotes role
T1515 5873-5875 IN denotes in
T1516 5876-5881 NN denotes iTreg
T1517 5882-5886 NN denotes cell
T1518 5887-5897 NN denotes commitment
T1519 5898-5900 IN denotes as
T1520 5901-5906 NN denotes T-bet
T1521 5907-5910 CC denotes and
T1522 5911-5916 NN denotes GATA3
T1523 5917-5920 IN denotes for
T1524 5921-5924 NN denotes Th1
T1525 5925-5928 CC denotes and
T1526 5929-5932 NN denotes Th2
T1527 5933-5948 NN denotes differentiation
T1528 5948-5949 -COMMA- denotes ,
T1529 5950-5962 RB denotes respectively
T1530 5964-5966 PRP denotes We
T1531 5967-5971 VB denotes show
T1532 5972-5976 IN denotes that
T1533 5977-5982 NN denotes GATA3
T1534 5983-5991 VB denotes inhibits
T1535 5992-5997 NN denotes FOXP3
T1536 5998-6007 NN denotes induction
T1537 6008-6011 CC denotes and
T1538 6012-6016 IN denotes that
T1539 6017-6021 NN denotes IL-4
T1540 6022-6028 VB denotes limits
T1541 6029-6034 NN denotes FOXP3
T1542 6035-6045 NN denotes expression
T1543 6046-6048 IN denotes in
T1544 6049-6050 DT denotes a
T1545 6051-6065 JJ denotes GATA3-mediated
T1546 6066-6069 NN denotes way
T1547 6069-6070 -COMMA- denotes ,
T1548 6071-6075 CC denotes both
T1549 6076-6078 FW denotes in
T1550 6079-6084 FW denotes vitro
T1551 6085-6088 CC denotes and
T1552 6089-6091 FW denotes in
T1553 6092-6096 FW denotes vivo
T1554 6098-6100 PRP denotes We
T1555 6101-6105 RB denotes also
T1556 6106-6110 VB denotes show
T1557 6111-6115 IN denotes that
T1558 6116-6121 NN denotes GATA3
T1559 6122-6130 RB denotes directly
T1560 6131-6136 VB denotes binds
T1561 6137-6139 TO denotes to
T1562 6140-6143 DT denotes the
T1563 6144-6149 NN denotes FOXP3
T1564 6150-6158 NN denotes promoter
T1565 6159-6162 CC denotes and
T1566 6163-6170 RB denotes thereby
T1567 6171-6179 VB denotes prevents
T1568 6180-6183 DT denotes the
T1569 6184-6193 NN denotes induction
T1570 6194-6196 IN denotes of
T1571 6197-6201 DT denotes this
T1572 6202-6206 NN denotes gene
T1573 6206-6207 -COMMA- denotes ,
T1574 6208-6221 VB denotes demonstrating
T1575 6222-6226 IN denotes that
T1576 6227-6230 NN denotes Th2
T1577 6231-6246 NN denotes differentiation
T1578 6247-6256 VB denotes overrules
T1579 6257-6262 NN denotes iTreg
T1580 6263-6272 NN denotes induction
T3016 6284-6289 NN denotes FOXP3
T3017 6290-6299 NN denotes Induction
T3018 6300-6302 IN denotes in
T3019 6303-6304 NN denotes T
T3020 6305-6309 NN denotes Cell
T3021 6310-6317 NN denotes Subsets
T3022 6318-6320 PRP denotes It
T3023 6321-6323 VB denotes is
T3024 6324-6331 VB denotes assumed
T3025 6332-6336 IN denotes that
T3026 6337-6342 NN denotes FOXP3
T3027 6343-6353 NN denotes expression
T3028 6354-6357 MD denotes can
T3029 6358-6360 VB denotes be
T3030 6361-6368 VB denotes induced
T3031 6369-6371 IN denotes in
T3032 6372-6385 JJ denotes nonregulatory
T3033 6386-6387 NN denotes T
T3034 6388-6393 NN denotes cells
T3035 6393-6394 -COMMA- denotes ,
T3036 6395-6400 WDT denotes which
T3037 6401-6403 VB denotes is
T3038 6404-6406 DT denotes an
T3039 6407-6416 JJ denotes important
T3040 6417-6421 NN denotes step
T3041 6422-6424 IN denotes in
T3042 6425-6430 NN denotes iTreg
T3043 6431-6435 NN denotes cell
T3044 6436-6451 NN denotes differentiation
T3045 6453-6460 RB denotes However
T3046 6460-6461 -COMMA- denotes ,
T3047 6462-6464 PRP denotes it
T3048 6465-6467 VB denotes is
T3049 6468-6471 RB denotes not
T3050 6472-6477 VB denotes known
T3051 6478-6480 IN denotes if
T3052 6481-6484 DT denotes all
T3053 6485-6489 JJ denotes CD4+
T3054 6490-6491 NN denotes T
T3055 6492-6497 NN denotes cells
T3056 6498-6502 VB denotes have
T3057 6503-6506 DT denotes the
T3058 6507-6511 JJ denotes same
T3059 6512-6520 NN denotes capacity
T3060 6521-6523 TO denotes to
T3061 6524-6531 VB denotes express
T3062 6532-6537 NN denotes FOXP3
T3063 6539-6541 TO denotes To
T3064 6542-6553 VB denotes investigate
T3065 6554-6561 IN denotes whether
T3066 6562-6567 NN denotes FOXP3
T3067 6568-6571 MD denotes can
T3068 6572-6574 VB denotes be
T3069 6575-6584 VB denotes expressed
T3070 6585-6587 IN denotes by
T3071 6588-6591 DT denotes any
T3072 6592-6593 NN denotes T
T3073 6594-6598 NN denotes cell
T3074 6599-6605 NN denotes subset
T3075 6606-6608 CC denotes or
T3076 6609-6611 IN denotes if
T3077 6612-6622 NN denotes expression
T3078 6623-6625 VB denotes is
T3079 6626-6636 JJ denotes restricted
T3080 6637-6639 TO denotes to
T3081 6640-6641 DT denotes a
T3082 6642-6650 JJ denotes distinct
T3083 6651-6658 NN denotes lineage
T3084 6658-6659 -COMMA- denotes ,
T3085 6660-6665 NN denotes FOXP3
T3086 6666-6670 NN denotes mRNA
T3087 6671-6681 NN denotes expression
T3088 6682-6685 VB denotes was
T3089 6686-6694 VB denotes analyzed
T3090 6695-6697 IN denotes in
T3091 6698-6705 RB denotes freshly
T3092 6706-6714 VB denotes isolated
T3093 6715-6716 NN denotes T
T3094 6717-6722 NN denotes cells
T3095 6723-6727 JJ denotes such
T3096 6728-6730 IN denotes as
T3097 6731-6744 JJ denotes CD25-depleted
T3098 6745-6749 JJ denotes CD4+
T3099 6750-6755 NN denotes cells
T3100 6755-6756 -COMMA- denotes ,
T3101 6757-6764 JJ denotes CD45RA+
T3102 6765-6770 JJ denotes naive
T3103 6771-6773 CC denotes or
T3104 6774-6781 JJ denotes CD45RO+
T3105 6782-6788 NN denotes memory
T3106 6789-6790 NN denotes T
T3107 6791-6796 NN denotes cells
T3108 6797-6798 -LRB- denotes (
T3109 6798-6804 NN denotes Figure
T3110 6805-6807 NN denotes 1A
T3111 6807-6808 -RRB- denotes )
T3112 6808-6809 -COMMA- denotes ,
T3113 6810-6812 RB denotes as
T3114 6813-6817 RB denotes well
T3115 6818-6820 IN denotes as
T3116 6821-6822 NN denotes T
T3117 6823-6828 NN denotes cells
T3118 6829-6835 VB denotes driven
T3119 6836-6838 FW denotes in
T3120 6839-6844 FW denotes vitro
T3121 6845-6851 IN denotes toward
T3122 6852-6855 NN denotes Th1
T3123 6855-6856 -COMMA- denotes ,
T3124 6857-6860 NN denotes Th2
T3125 6860-6861 -COMMA- denotes ,
T3126 6862-6864 CC denotes or
T3127 6865-6870 NN denotes iTreg
T3128 6871-6881 NN denotes phenotypes
T3129 6882-6883 -LRB- denotes (
T3130 6883-6889 NN denotes Figure
T3131 6890-6892 NN denotes 1B
T3132 6892-6893 -COLON- denotes ;
T3133 6894-6903 NN denotes phenotype
T3134 6904-6906 IN denotes on
T3135 6907-6913 NN denotes Figure
T3136 6914-6916 NN denotes S1
T3137 6916-6917 -RRB- denotes )
T3138 6919-6922 DT denotes The
T3139 6923-6932 NN denotes CD4+CD25–
T3140 6932-6933 -COMMA- denotes ,
T3141 6934-6941 JJ denotes CD45RA+
T3142 6941-6942 -COMMA- denotes ,
T3143 6943-6950 JJ denotes CD45RO+
T3144 6950-6951 -COMMA- denotes ,
T3145 6952-6955 CC denotes and
T3146 6956-6972 NN denotes CD4+CD45RO+CD25–
T3147 6973-6977 VB denotes were
T3148 6978-6982 JJ denotes able
T3149 6983-6985 TO denotes to
T3150 6986-6999 RB denotes significantly
T3151 7000-7006 VB denotes induce
T3152 7007-7012 NN denotes FOXP3
T3153 7013-7017 NN denotes mRNA
T3154 7018-7020 IN denotes up
T3155 7021-7023 TO denotes to
T3156 7024-7031 RB denotes 30-fold
T3157 7032-7036 IN denotes upon
T3158 7037-7040 NN denotes TCR
T3159 7041-7051 NN denotes activation
T3160 7052-7055 CC denotes and
T3161 7056-7064 NN denotes addition
T3162 7065-7067 IN denotes of
T3163 7068-7073 NN denotes TGF-β
T3164 7075-7078 NN denotes Th1
T3165 7079-7084 NN denotes cells
T3166 7085-7091 VB denotes showed
T3167 7092-7096 RB denotes only
T3168 7097-7098 DT denotes a
T3169 7099-7106 JJ denotes 10-fold
T3170 7107-7115 NN denotes increase
T3171 7117-7119 IN denotes In
T3172 7120-7128 NN denotes contrast
T3173 7128-7129 -COMMA- denotes ,
T3174 7130-7133 NN denotes Th2
T3175 7134-7139 NN denotes cells
T3176 7140-7150 VB denotes stimulated
T3177 7151-7156 IN denotes under
T3178 7157-7160 DT denotes the
T3179 7161-7165 JJ denotes same
T3180 7166-7176 NN denotes conditions
T3181 7177-7180 VB denotes did
T3182 7181-7184 RB denotes not
T3183 7185-7193 VB denotes increase
T3184 7194-7199 NN denotes FOXP3
T3185 7200-7210 NN denotes expression
T3186 7212-7215 DT denotes The
T3187 7216-7218 FW denotes in
T3188 7219-7224 FW denotes vitro
T3189 7225-7234 VB denotes generated
T3190 7235-7240 NN denotes iTreg
T3191 7241-7246 NN denotes cells
T3192 7247-7251 VB denotes were
T3193 7252-7258 JJ denotes unable
T3194 7259-7261 TO denotes to
T3195 7262-7269 RB denotes further
T3196 7270-7281 VB denotes up-regulate
T3197 7282-7287 NN denotes FOXP3
T3198 7287-7288 -COMMA- denotes ,
T3199 7289-7294 WDT denotes which
T3200 7295-7298 VB denotes was
T3201 7299-7306 RB denotes already
T3202 7307-7309 IN denotes at
T3203 7310-7314 JJ denotes high
T3204 7315-7321 NN denotes levels
T3205 7322-7327 IN denotes under
T3206 7328-7331 DT denotes the
T3207 7332-7339 JJ denotes resting
T3208 7340-7350 NN denotes conditions
T3209 7351-7352 -LRB- denotes (
T3210 7352-7358 NN denotes Figure
T3211 7359-7361 NN denotes 1B
T3212 7361-7362 -COMMA- denotes ,
T3213 7363-7368 JJ denotes right
T3214 7369-7374 NN denotes panel
T3215 7374-7375 -RRB- denotes )
T3216 7387-7390 NN denotes Th2
T3217 7391-7396 NN denotes Cells
T3218 7967-7970 VB denotes are
T3219 7971-7976 VB denotes known
T3220 7977-7979 TO denotes to
T3221 7980-7987 VB denotes produce
T3222 7988-7992 NN denotes IL-4
T3223 7993-7997 IN denotes upon
T3224 7998-8008 NN denotes activation
T3225 8008-8009 -COMMA- denotes ,
T3226 8010-8015 WDT denotes which
T3227 8016-8019 MD denotes may
T3228 8020-8028 VB denotes interact
T3229 8029-8033 IN denotes with
T3230 8034-8039 NN denotes TGF-β
T3231 8040-8049 NN denotes signaling
T3232 8050-8053 CC denotes and
T3233 8054-8058 RB denotes thus
T3234 8059-8066 VB denotes prevent
T3235 8067-8072 NN denotes FOXP3
T3236 8073-8082 NN denotes induction
T3237 8084-8091 RB denotes However
T3238 8091-8092 -COMMA- denotes ,
T3239 8093-8096 DT denotes the
T3240 8097-8111 NN denotes neutralization
T3241 8112-8114 IN denotes of
T3242 8115-8119 NN denotes IL-4
T3243 8120-8124 IN denotes with
T3244 8125-8126 DT denotes a
T3245 8127-8135 VB denotes blocking
T3246 8136-8140 NN denotes IL-4
T3247 8141-8149 NN denotes antibody
T3248 8150-8153 VB denotes did
T3249 8154-8157 RB denotes not
T3250 8158-8164 VB denotes rescue
T3251 8165-8170 NN denotes FOXP3
T3252 8171-8181 NN denotes expression
T3253 8182-8184 IN denotes in
T3254 8185-8188 DT denotes the
T3255 8189-8203 VB denotes differentiated
T3256 8204-8207 NN denotes Th2
T3257 8208-8213 NN denotes cells
T3258 8214-8215 -LRB- denotes (
T3259 8215-8226 JJ denotes unpublished
T3260 8227-8231 NN denotes data
T3261 8231-8232 -RRB- denotes )
T3262 8234-8239 DT denotes These
T3263 8240-8244 NN denotes data
T3264 8245-8257 VB denotes demonstrated
T3265 8258-8262 IN denotes that
T3266 8263-8266 NN denotes Th2
T3267 8267-8272 NN denotes cells
T3268 8273-8277 VB denotes have
T3269 8278-8279 DT denotes a
T3270 8280-8287 VB denotes limited
T3271 8288-8296 NN denotes capacity
T3272 8297-8299 TO denotes to
T3273 8300-8307 VB denotes express
T3274 8308-8313 NN denotes FOXP3
T3275 8314-8315 -LRB- denotes (
T3276 8315-8321 NN denotes Figure
T3277 8322-8324 NN denotes 1B
T3278 8324-8325 -RRB- denotes )
T3279 8327-8330 DT denotes The
T3280 8331-8340 NN denotes inability
T3281 8341-8343 IN denotes of
T3282 8344-8347 NN denotes Th2
T3283 8348-8353 NN denotes cells
T3284 8354-8356 TO denotes to
T3285 8357-8364 VB denotes express
T3286 8365-8370 NN denotes FOXP3
T3287 8371-8374 VB denotes was
T3288 8375-8379 RB denotes also
T3289 8380-8390 VB denotes documented
T3290 8391-8393 IN denotes at
T3291 8394-8397 DT denotes the
T3292 8398-8409 JJ denotes single-cell
T3293 8410-8415 NN denotes level
T3294 8415-8416 -COMMA- denotes ,
T3295 8417-8427 VB denotes confirming
T3296 8428-8432 IN denotes that
T3297 8433-8436 NN denotes Th2
T3298 8437-8442 NN denotes cells
T3299 8443-8447 VB denotes lack
T3300 8448-8453 NN denotes FOXP3
T3301 8454-8464 NN denotes expression
T3302 8465-8466 -LRB- denotes (
T3303 8466-8472 NN denotes Figure
T3304 8473-8475 NN denotes 2A
T3305 8475-8476 -RRB- denotes )
T3306 8478-8482 RB denotes Only
T3307 8483-8488 NN denotes iTreg
T3308 8489-8494 NN denotes cells
T3309 8495-8504 VB denotes expressed
T3310 8505-8510 NN denotes FOXP3
T3311 8511-8513 IN denotes in
T3312 8514-8521 VB denotes resting
T3313 8522-8532 NN denotes conditions
T3314 8534-8547 RB denotes Interestingly
T3315 8547-8548 -COMMA- denotes ,
T3316 8549-8551 PRP denotes we
T3317 8552-8560 VB denotes observed
T3318 8561-8565 IN denotes that
T3319 8566-8573 VB denotes resting
T3320 8574-8579 NN denotes iTreg
T3321 8580-8585 NN denotes cells
T3322 8586-8593 VB denotes express
T3323 8594-8599 NN denotes FOXP3
T3324 8600-8603 CC denotes but
T3325 8604-8608 VB denotes show
T3326 8609-8612 JJ denotes low
T3327 8613-8617 NN denotes CD25
T3328 8618-8625 NN denotes surface
T3329 8626-8636 NN denotes expression
T3330 8638-8646 VB denotes Repeated
T3331 8647-8655 NN denotes exposure
T3332 8656-8658 TO denotes to
T3333 8659-8664 NN denotes TGF-β
T3334 8665-8668 VB denotes did
T3335 8669-8672 RB denotes not
T3336 8673-8680 RB denotes further
T3337 8681-8689 VB denotes increase
T3338 8690-8693 DT denotes the
T3339 8694-8699 NN denotes FOXP3
T3340 8700-8710 NN denotes expression
T3341 8711-8713 IN denotes in
T3342 8714-8717 DT denotes the
T3343 8718-8723 NN denotes iTreg
T3344 8724-8731 NN denotes lineage
T3345 8732-8735 CC denotes but
T3346 8736-8747 RB denotes transiently
T3347 8748-8755 VB denotes induced
T3348 8756-8761 NN denotes FOXP3
T3349 8762-8772 NN denotes expression
T3350 8773-8775 IN denotes in
T3351 8776-8779 NN denotes Th1
T3352 8780-8785 NN denotes cells
T3353 8787-8796 RB denotes Naturally
T3354 8797-8806 VB denotes occurring
T3355 8807-8810 NN denotes Th2
T3356 8811-8816 NN denotes cells
T3357 8817-8821 JJ denotes such
T3358 8822-8824 IN denotes as
T3359 8825-8831 JJ denotes CRTH2+
T3360 8832-8833 NN denotes T
T3361 8834-8839 NN denotes cells
T3362 8839-8840 -COMMA- denotes ,
T3363 8841-8842 NN denotes T
T3364 8843-8848 NN denotes cells
T3365 8849-8857 VB denotes isolated
T3366 8858-8867 VB denotes according
T3367 8868-8870 TO denotes to
T3368 8871-8876 PRP-DOLLAR- denotes their
T3369 8877-8881 NN denotes IL-4
T3370 8882-8891 NN denotes secretion
T3371 8891-8892 -COMMA- denotes ,
T3372 8893-8895 CC denotes or
T3373 8896-8898 DT denotes an
T3374 8899-8913 JJ denotes IL-4–producing
T3375 8914-8915 NN denotes T
T3376 8916-8920 NN denotes cell
T3377 8921-8926 NN denotes clone
T3378 8927-8928 -LRB- denotes (
T3379 8928-8931 NN denotes BR8
T3380 8931-8932 -RRB- denotes )
T3381 8933-8937 VB denotes were
T3382 8938-8942 RB denotes also
T3383 8943-8950 VB denotes lacking
T3384 8951-8956 NN denotes FOXP3
T3385 8957-8967 NN denotes expression
T3386 8968-8969 -LRB- denotes (
T3387 8969-8975 NN denotes Figure
T3388 8976-8978 NN denotes 2B
T3389 8978-8979 -RRB- denotes )
T3390 8981-8992 RB denotes Furthermore
T3391 8993-9007 JJ denotes TGF-β–mediated
T3392 9008-9013 NN denotes FOXP3
T3393 9014-9023 NN denotes induction
T3394 9024-9030 VB denotes failed
T3395 9031-9033 IN denotes in
T3396 9034-9039 DT denotes these
T3397 9040-9045 NN denotes cells
T3398 9046-9048 IN denotes in
T3399 9049-9057 NN denotes contrast
T3400 9058-9060 TO denotes to
T3401 9061-9064 DT denotes the
T3402 9065-9070 JJ denotes naive
T3403 9071-9072 NN denotes T
T3404 9073-9078 NN denotes cells
T3405 9079-9080 -LRB- denotes (
T3406 9080-9086 NN denotes Figure
T3407 9087-9089 NN denotes 2B
T3408 9089-9090 -RRB- denotes )
T3409 9092-9099 IN denotes Because
T3410 9100-9104 NN denotes IL-4
T3411 9105-9107 VB denotes is
T3412 9108-9111 DT denotes the
T3413 9112-9115 JJ denotes key
T3414 9116-9119 NN denotes Th2
T3415 9120-9128 NN denotes cytokine
T3416 9128-9129 -COMMA- denotes ,
T3417 9130-9133 DT denotes the
T3418 9134-9144 NN denotes expression
T3419 9145-9147 IN denotes of
T3420 9148-9152 NN denotes IL-4
T3421 9153-9156 CC denotes and
T3422 9157-9162 NN denotes FOXP3
T3423 9163-9165 IN denotes in
T3424 9166-9173 RB denotes freshly
T3425 9174-9182 VB denotes isolated
T3426 9183-9187 JJ denotes CD4+
T3427 9188-9189 NN denotes T
T3428 9190-9195 NN denotes cells
T3429 9196-9199 VB denotes was
T3430 9200-9208 VB denotes analyzed
T3431 9209-9211 IN denotes by
T3432 9212-9224 NN denotes fluorescence
T3433 9225-9234 VB denotes activated
T3434 9235-9239 NN denotes cell
T3435 9240-9247 NN denotes sorting
T3436 9248-9249 -LRB- denotes (
T3437 9249-9253 NN denotes FACS
T3438 9253-9254 -RRB- denotes )
T3439 9256-9271 JJ denotes IL-4–expressing
T3440 9272-9277 NN denotes cells
T3441 9278-9282 VB denotes were
T3442 9283-9287 RB denotes most
T3443 9288-9296 JJ denotes abundant
T3444 9297-9302 IN denotes among
T3445 9303-9315 NN denotes CD45RO+CD25–
T3446 9316-9321 NN denotes cells
T3447 9321-9322 -COMMA- denotes ,
T3448 9323-9328 WDT denotes which
T3449 9329-9332 VB denotes did
T3450 9333-9336 RB denotes not
T3451 9337-9347 VB denotes co-express
T3452 9348-9353 NN denotes FOXP3
T3453 9354-9355 -LRB- denotes (
T3454 9355-9361 NN denotes Figure
T3455 9362-9364 NN denotes 3A
T3456 9364-9365 -COMMA- denotes ,
T3457 9366-9370 JJ denotes left
T3458 9371-9376 NN denotes panel
T3459 9376-9377 -RRB- denotes )
T3460 9379-9381 IN denotes In
T3461 9382-9390 NN denotes contrast
T3462 9390-9391 -COMMA- denotes ,
T3463 9392-9404 JJ denotes CD45RO+CD25+
T3464 9405-9410 NN denotes cells
T3465 9411-9421 RB denotes abundantly
T3466 9422-9431 VB denotes expressed
T3467 9432-9437 NN denotes FOXP3
T3468 9437-9438 -COMMA- denotes ,
T3469 9439-9444 IN denotes while
T3470 9445-9452 VB denotes lacking
T3471 9453-9457 NN denotes IL-4
T3472 9458-9459 -LRB- denotes (
T3473 9459-9465 NN denotes Figure
T3474 9466-9468 NN denotes 3A
T3475 9468-9469 -COMMA- denotes ,
T3476 9470-9475 JJ denotes right
T3477 9476-9481 NN denotes panel
T3478 9481-9482 -RRB- denotes )
T3479 9484-9486 IN denotes As
T3480 9487-9492 VB denotes shown
T3481 9493-9495 IN denotes in
T3482 9496-9502 NN denotes Figure
T3483 9503-9505 NN denotes 3B
T3484 9505-9506 -COMMA- denotes ,
T3485 9507-9510 DT denotes the
T3486 9511-9520 NN denotes frequency
T3487 9521-9523 IN denotes of
T3488 9524-9527 DT denotes the
T3489 9528-9533 JJ denotes IL-4+
T3490 9534-9539 NN denotes cells
T3491 9540-9543 VB denotes was
T3492 9544-9550 RB denotes always
T3493 9551-9556 IN denotes below
T3494 9557-9558 CD denotes 1
T3495 9558-9559 NN denotes %
T3496 9560-9562 IN denotes in
T3497 9563-9566 DT denotes the
T3498 9567-9573 JJ denotes FOXP3+
T3499 9574-9579 NN denotes cells
T3500 9580-9585 RB denotes close
T3501 9586-9588 TO denotes to
T3502 9589-9592 DT denotes the
T3503 9593-9603 NN denotes background
T3504 9605-9608 DT denotes The
T3505 9609-9614 JJ denotes IL-4+
T3506 9615-9620 NN denotes cells
T3507 9621-9625 VB denotes were
T3508 9626-9634 VB denotes confined
T3509 9635-9637 TO denotes to
T3510 9638-9641 DT denotes the
T3511 9642-9648 NN denotes FOXP3–
T3512 9649-9654 NN denotes cells
T3513 9654-9655 -COMMA- denotes ,
T3514 9656-9658 IN denotes as
T3515 9659-9664 VB denotes shown
T3516 9665-9668 IN denotes for
T3517 9669-9672 DT denotes the
T3518 9673-9685 NN denotes CD45RO+CD25–
T3519 9685-9686 -COMMA- denotes ,
T3520 9687-9699 JJ denotes CD45RO+CD25+
T3521 9699-9700 -COMMA- denotes ,
T3522 9701-9704 CC denotes and
T3523 9705-9721 JJ denotes CD45RO+CD25+high
T3524 9722-9727 NN denotes cells
T3525 9728-9729 -LRB- denotes (
T3526 9729-9735 NN denotes Figure
T3527 9736-9738 NN denotes 3B
T3528 9738-9739 -RRB- denotes )
T3529 9741-9743 IN denotes In
T3530 9744-9752 NN denotes addition
T3531 9752-9753 -COMMA- denotes ,
T3532 9754-9761 CC denotes neither
T3533 9762-9765 DT denotes the
T3534 9766-9769 NN denotes Th2
T3535 9770-9775 NN denotes clone
T3536 9776-9777 -LRB- denotes (
T3537 9777-9780 NN denotes BR8
T3538 9780-9781 -RRB- denotes )
T3539 9782-9785 CC denotes nor
T3540 9786-9791 NN denotes CRTH2
T3541 9792-9797 NN denotes cells
T3542 9798-9811 RB denotes significantly
T3543 9812-9821 VB denotes expressed
T3544 9822-9827 NN denotes FOXP3
T3545 9828-9829 -LRB- denotes (
T3546 9829-9835 NN denotes Figure
T3547 9836-9838 NN denotes 3C
T3548 9838-9839 -RRB- denotes )
T3549 9841-9846 NN denotes Cells
T3550 9847-9855 VB denotes enriched
T3551 9856-9859 IN denotes for
T3552 9860-9865 PRP-DOLLAR- denotes their
T3553 9866-9870 NN denotes IL-4
T3554 9871-9880 NN denotes secretion
T3555 9881-9886 VB denotes using
T3556 9887-9890 DT denotes the
T3557 9891-9899 JJ denotes magnetic
T3558 9900-9904 NN denotes cell
T3559 9905-9914 NN denotes isolation
T3560 9915-9925 NN denotes technology
T3561 9926-9935 VB denotes contained
T3562 9936-9940 DT denotes some
T3563 9941-9957 JJ denotes FOXP3-expressing
T3564 9958-9963 NN denotes cells
T3565 9963-9964 -COMMA- denotes ,
T3566 9965-9968 CC denotes but
T3567 9969-9980 RB denotes importantly
T3568 9980-9981 -COMMA- denotes ,
T3569 9982-9985 DT denotes the
T3570 9986-9996 NN denotes expression
T3571 9997-10000 VB denotes did
T3572 10001-10004 RB denotes not
T3573 10005-10012 VB denotes overlap
T3574 10014-10019 VB denotes Taken
T3575 10020-10028 RB denotes together
T3576 10028-10029 -COMMA- denotes ,
T3577 10030-10035 DT denotes these
T3578 10036-10040 NN denotes data
T3579 10041-10049 VB denotes indicate
T3580 10050-10054 IN denotes that
T3581 10055-10060 NN denotes FOXP3
T3582 10061-10064 VB denotes was
T3583 10065-10068 RB denotes not
T3584 10069-10078 VB denotes expressed
T3585 10079-10081 IN denotes by
T3586 10082-10085 NN denotes Th2
T3587 10086-10091 NN denotes cells
T3588 10092-10095 CC denotes and
T3589 10096-10099 VB denotes was
T3590 10100-10103 RB denotes not
T3591 10104-10113 JJ denotes inducible
T3592 10114-10116 IN denotes in
T3593 10117-10122 DT denotes those
T3594 10123-10128 NN denotes cells
T5221 11403-11408 NN denotes FOXP3
T5222 11409-11412 CC denotes and
T5223 11413-11418 NN denotes GATA3
T5224 11419-11427 NN denotes Kinetics
T5225 11428-11430 IN denotes in
T5226 11431-11446 VB denotes Differentiating
T5227 11447-11452 NN denotes Cells
T5228 11453-11456 DT denotes The
T5229 11457-11464 VB denotes limited
T5230 11465-11473 NN denotes capacity
T5231 11474-11476 IN denotes of
T5232 11477-11491 VB denotes differentiated
T5233 11492-11500 NN denotes effector
T5234 11501-11506 NN denotes cells
T5235 11507-11509 TO denotes to
T5236 11510-11516 VB denotes induce
T5237 11517-11522 NN denotes FOXP3
T5238 11523-11533 NN denotes expression
T5239 11534-11542 VB denotes suggests
T5240 11543-11547 IN denotes that
T5241 11548-11553 NN denotes iTreg
T5242 11554-11563 NN denotes induction
T5243 11564-11567 VB denotes has
T5244 11568-11570 TO denotes to
T5245 11571-11576 VB denotes occur
T5246 11577-11583 IN denotes before
T5247 11584-11592 NN denotes effector
T5248 11593-11594 NN denotes T
T5249 11595-11599 NN denotes cell
T5250 11600-11615 NN denotes differentiation
T5251 11616-11622 VB denotes occurs
T5252 11624-11633 RB denotes Therefore
T5253 11633-11634 -COMMA- denotes ,
T5254 11635-11637 PRP denotes we
T5255 11638-11646 VB denotes analyzed
T5256 11647-11650 DT denotes the
T5257 11651-11661 NN denotes expression
T5258 11662-11664 IN denotes of
T5259 11665-11670 NN denotes FOXP3
T5260 11671-11674 CC denotes and
T5261 11675-11680 NN denotes GATA3
T5262 11681-11687 IN denotes during
T5263 11688-11691 DT denotes the
T5264 11692-11707 NN denotes differentiation
T5265 11708-11710 IN denotes of
T5266 11711-11716 JJ denotes naive
T5267 11717-11720 NN denotes CD4
T5268 11721-11722 NN denotes T
T5269 11723-11728 NN denotes cells
T5270 11729-11733 IN denotes into
T5271 11734-11737 NN denotes Th0
T5272 11738-11739 -LRB- denotes (
T5273 11739-11746 JJ denotes neutral
T5274 11746-11747 -COMMA- denotes ,
T5275 11748-11757 JJ denotes anti-IL-4
T5276 11757-11758 -COMMA- denotes ,
T5277 11759-11769 JJ denotes anti-IFN-γ
T5278 11769-11770 -COMMA- denotes ,
T5279 11771-11774 CC denotes and
T5280 11775-11779 JJ denotes anti
T5281 11780-11785 NN denotes IL-12
T5282 11785-11786 -RRB- denotes )
T5283 11786-11787 -COMMA- denotes ,
T5284 11788-11791 NN denotes Th2
T5285 11791-11792 -COMMA- denotes ,
T5286 11793-11796 CC denotes and
T5287 11797-11802 NN denotes iTreg
T5288 11803-11813 NN denotes phenotypes
T5289 11815-11820 IN denotes After
T5290 11821-11831 NN denotes initiation
T5291 11832-11834 IN denotes of
T5292 11835-11838 DT denotes the
T5293 11839-11854 NN denotes differentiation
T5294 11855-11862 NN denotes process
T5295 11862-11863 -COMMA- denotes ,
T5296 11864-11869 NN denotes FOXP3
T5297 11870-11873 CC denotes and
T5298 11874-11879 NN denotes GATA3
T5299 11880-11886 VB denotes showed
T5300 11887-11888 DT denotes a
T5301 11889-11896 JJ denotes similar
T5302 11897-11907 NN denotes expression
T5303 11908-11915 JJ denotes kinetic
T5304 11916-11922 IN denotes within
T5305 11923-11926 DT denotes the
T5306 11927-11932 JJ denotes first
T5307 11933-11934 CD denotes 3
T5308 11935-11936 NN denotes d
T5309 11936-11937 -COMMA- denotes ,
T5310 11938-11943 WDT denotes which
T5311 11944-11947 VB denotes are
T5312 11948-11958 VB denotes considered
T5313 11959-11961 TO denotes to
T5314 11962-11964 VB denotes be
T5315 11965-11973 JJ denotes critical
T5316 11974-11976 IN denotes in
T5317 11977-11978 NN denotes T
T5318 11979-11983 NN denotes cell
T5319 11984-11994 NN denotes commitment
T5320 11995-11996 -LRB- denotes [
T5321 11996-11998 CD denotes 18
T5322 11998-11999 -RRB- denotes ]
T5323 12001-12006 IN denotes Under
T5324 12007-12010 NN denotes Th2
T5325 12011-12026 NN denotes differentiation
T5326 12027-12037 NN denotes conditions
T5327 12037-12038 -COMMA- denotes ,
T5328 12039-12044 NN denotes FOXP3
T5329 12045-12049 NN denotes mRNA
T5330 12050-12060 NN denotes expression
T5331 12061-12070 VB denotes increased
T5332 12071-12075 RB denotes only
T5333 12076-12086 RB denotes marginally
T5334 12087-12088 -LRB- denotes (
T5335 12088-12094 NN denotes Figure
T5336 12095-12097 NN denotes 4A
T5337 12097-12098 -COMMA- denotes ,
T5338 12099-12103 JJ denotes left
T5339 12104-12109 NN denotes panel
T5340 12109-12110 -RRB- denotes )
T5341 12112-12116 RB denotes Thus
T5342 12116-12117 -COMMA- denotes ,
T5343 12118-12126 IN denotes although
T5344 12127-12132 NN denotes GATA3
T5345 12133-12136 CC denotes and
T5346 12137-12142 NN denotes FOXP3
T5347 12143-12149 VB denotes showed
T5348 12150-12157 JJ denotes similar
T5349 12158-12166 NN denotes kinetics
T5350 12166-12167 -COMMA- denotes ,
T5351 12168-12173 PRP-DOLLAR- denotes their
T5352 12174-12184 NN denotes expression
T5353 12185-12194 VB denotes polarizes
T5354 12195-12197 IN denotes at
T5355 12198-12201 DT denotes the
T5356 12202-12205 NN denotes end
T5357 12206-12208 IN denotes of
T5358 12209-12212 DT denotes the
T5359 12213-12228 NN denotes differentiation
T5360 12229-12236 NN denotes process
T5361 12237-12241 WRB denotes when
T5362 12242-12247 NN denotes cells
T5363 12248-12252 VB denotes were
T5364 12253-12261 VB denotes cultured
T5365 12262-12269 IN denotes towards
T5366 12270-12273 NN denotes Th2
T5367 12274-12276 CC denotes or
T5368 12277-12282 NN denotes iTreg
T5369 12283-12288 NN denotes cells
T5370 12288-12289 -COMMA- denotes ,
T5371 12290-12302 RB denotes respectively
T5372 12303-12304 -LRB- denotes (
T5373 12304-12310 NN denotes Figure
T5374 12311-12313 NN denotes 4A
T5375 12314-12317 CC denotes and
T5376 12318-12320 NN denotes 4B
T5377 12320-12321 -RRB- denotes )
T5378 12323-12336 RB denotes Interestingly
T5379 12336-12337 -COMMA- denotes ,
T5380 12338-12341 DT denotes the
T5381 12342-12345 NN denotes Th0
T5382 12346-12351 NN denotes cells
T5383 12352-12356 VB denotes were
T5384 12357-12367 VB denotes expressing
T5385 12368-12372 JJ denotes more
T5386 12373-12378 NN denotes FOXP3
T5387 12379-12383 IN denotes than
T5388 12384-12387 DT denotes the
T5389 12388-12391 NN denotes Th2
T5390 12392-12397 NN denotes cells
T5391 12397-12398 -COMMA- denotes ,
T5392 12399-12402 CC denotes but
T5393 12403-12412 VB denotes expressed
T5394 12413-12416 JJ denotes low
T5395 12417-12423 NN denotes levels
T5396 12424-12426 IN denotes of
T5397 12427-12432 NN denotes GATA3
T5398 12432-12433 -COLON- denotes ;
T5399 12434-12441 RB denotes however
T5400 12441-12442 -COMMA- denotes ,
T5401 12443-12446 DT denotes the
T5402 12447-12454 NN denotes protein
T5403 12455-12465 NN denotes expression
T5404 12466-12474 RB denotes slightly
T5405 12475-12483 VB denotes differed
T5406 12484-12488 IN denotes from
T5407 12489-12493 NN denotes mRNA
T5408 12494-12504 NN denotes expression
T5409 12504-12505 -COMMA- denotes ,
T5410 12506-12516 VB denotes suggesting
T5411 12517-12521 RB denotes also
T5412 12522-12539 JJ denotes posttranslational
T5413 12540-12550 NN denotes regulation
T5414 12551-12554 CC denotes and
T5415 12555-12566 NN denotes degradation
T5416 12567-12569 IN denotes as
T5417 12570-12579 JJ denotes potential
T5418 12580-12590 JJ denotes additional
T5419 12591-12601 NN denotes mechanisms
T5420 12602-12604 IN denotes in
T5421 12605-12608 DT denotes the
T5422 12609-12624 NN denotes differentiation
T5423 12625-12632 NN denotes process
T5424 12634-12637 DT denotes The
T5425 12638-12647 NN denotes phenotype
T5426 12648-12650 IN denotes of
T5427 12651-12656 NN denotes iTreg
T5428 12657-12662 NN denotes cells
T5429 12663-12671 VB denotes included
T5430 12672-12674 DT denotes an
T5431 12675-12682 JJ denotes anergic
T5432 12683-12692 NN denotes phenotype
T5433 12693-12697 IN denotes upon
T5434 12698-12706 JJ denotes anti-CD3
T5435 12707-12721 NN denotes re-stimulation
T5436 12722-12723 -LRB- denotes (
T5437 12723-12729 NN denotes Figure
T5438 12730-12733 NN denotes S1A
T5439 12733-12734 -RRB- denotes )
T5440 12734-12735 -COMMA- denotes ,
T5441 12736-12741 NN denotes CD103
T5442 12741-12742 -COMMA- denotes ,
T5443 12743-12749 NN denotes CTLA-4
T5444 12749-12750 -COMMA- denotes ,
T5445 12751-12755 NN denotes GITR
T5446 12755-12756 -COMMA- denotes ,
T5447 12757-12760 CC denotes and
T5448 12761-12765 NN denotes PD-1
T5449 12766-12773 NN denotes surface
T5450 12774-12784 NN denotes expression
T5451 12785-12786 -LRB- denotes (
T5452 12786-12792 NN denotes Figure
T5453 12793-12796 NN denotes S1B
T5454 12796-12797 -RRB- denotes )
T5455 12799-12801 IN denotes On
T5456 12802-12805 DT denotes the
T5457 12806-12817 JJ denotes single-cell
T5458 12818-12823 NN denotes level
T5459 12823-12824 -COMMA- denotes ,
T5460 12825-12827 PRP denotes it
T5461 12828-12831 MD denotes can
T5462 12832-12834 VB denotes be
T5463 12835-12839 VB denotes seen
T5464 12840-12844 IN denotes that
T5465 12845-12850 NN denotes cells
T5466 12851-12859 VB denotes progress
T5467 12860-12867 IN denotes through
T5468 12868-12869 DT denotes a
T5469 12870-12880 NN denotes transition
T5470 12881-12886 NN denotes phase
T5471 12886-12887 -COMMA- denotes ,
T5472 12888-12893 WRB denotes where
T5473 12894-12899 NN denotes GATA3
T5474 12900-12903 CC denotes and
T5475 12904-12909 NN denotes FOXP3
T5476 12910-12920 NN denotes expression
T5477 12921-12928 NN denotes coexist
T5478 12929-12931 TO denotes to
T5479 12932-12936 DT denotes some
T5480 12937-12943 NN denotes degree
T5481 12944-12946 IN denotes in
T5482 12947-12950 DT denotes the
T5483 12951-12955 JJ denotes same
T5484 12956-12961 NN denotes cells
T5485 12961-12962 -COMMA- denotes ,
T5486 12963-12968 WDT denotes which
T5487 12969-12971 VB denotes is
T5488 12972-12980 VB denotes resolved
T5489 12981-12983 IN denotes in
T5490 12984-12989 NN denotes iTreg
T5491 12990-12995 NN denotes cells
T5492 12996-13001 IN denotes after
T5493 13002-13003 CD denotes 7
T5494 13004-13005 NN denotes d
T5495 13006-13007 -LRB- denotes (
T5496 13007-13013 NN denotes Figure
T5497 13014-13016 NN denotes 4B
T5498 13016-13017 -RRB- denotes )
T5499 13019-13024 VB denotes Taken
T5500 13025-13033 RB denotes together
T5501 13033-13034 -COMMA- denotes ,
T5502 13035-13040 DT denotes these
T5503 13041-13045 NN denotes data
T5504 13046-13058 VB denotes demonstrated
T5505 13059-13063 IN denotes that
T5506 13064-13067 NN denotes Th2
T5507 13068-13073 NN denotes cells
T5508 13074-13078 VB denotes have
T5509 13079-13083 VB denotes lost
T5510 13084-13089 PRP-DOLLAR- denotes their
T5511 13090-13098 NN denotes capacity
T5512 13099-13101 TO denotes to
T5513 13102-13109 VB denotes express
T5514 13110-13115 NN denotes FOXP3
T5515 13116-13119 CC denotes and
T5516 13120-13126 VB denotes showed
T5517 13127-13131 IN denotes that
T5518 13132-13135 NN denotes Th2
T5519 13136-13139 CC denotes and
T5520 13140-13145 NN denotes iTreg
T5521 13146-13151 NN denotes cells
T5522 13152-13157 VB denotes arise
T5523 13158-13162 IN denotes from
T5524 13163-13166 CD denotes two
T5525 13167-13176 JJ denotes different
T5526 13177-13192 NN denotes differentiation
T5527 13193-13201 NN denotes pathways
T6489 13785-13789 NN denotes IL-4
T6490 13790-13798 VB denotes Inhibits
T6491 13799-13813 JJ denotes TGF-β-Mediated
T6492 13814-13819 NN denotes iTreg
T6493 13820-13830 NNP denotes Commitment
T6494 13831-13835 NN denotes IL-4
T6495 13836-13843 VB denotes induces
T6496 13844-13859 NN denotes differentiation
T6497 13860-13862 IN denotes of
T6498 13863-13868 JJ denotes naive
T6499 13869-13870 NN denotes T
T6500 13871-13876 NN denotes cells
T6501 13876-13877 -COMMA- denotes ,
T6502 13878-13882 IN denotes upon
T6503 13883-13890 NN denotes antigen
T6504 13891-13900 NN denotes encounter
T6505 13900-13901 -COMMA- denotes ,
T6506 13902-13906 IN denotes into
T6507 13907-13910 DT denotes the
T6508 13911-13914 NN denotes Th2
T6509 13915-13919 NN denotes cell
T6510 13920-13927 NN denotes lineage
T6511 13929-13931 PRP denotes We
T6512 13932-13941 RB denotes therefore
T6513 13942-13947 VB denotes asked
T6514 13948-13955 IN denotes whether
T6515 13956-13960 NN denotes IL-4
T6516 13961-13963 VB denotes is
T6517 13964-13968 JJ denotes able
T6518 13969-13971 TO denotes to
T6519 13972-13979 VB denotes inhibit
T6520 13980-13985 NN denotes TGF-β
T6521 13986-13995 NN denotes induction
T6522 13996-13998 IN denotes of
T6523 13999-14004 NN denotes FOXP3
T6524 14005-14011 IN denotes during
T6525 14012-14015 DT denotes the
T6526 14016-14023 NN denotes priming
T6527 14024-14026 IN denotes of
T6528 14027-14032 JJ denotes naive
T6529 14033-14034 NN denotes T
T6530 14035-14040 NN denotes cells
T6531 14042-14047 JJ denotes Human
T6532 14048-14059 JJ denotes CD4+CD45RA+
T6533 14060-14061 NN denotes T
T6534 14062-14067 NN denotes cells
T6535 14068-14072 VB denotes were
T6536 14073-14082 VB denotes activated
T6537 14083-14087 IN denotes with
T6538 14088-14099 JJ denotes plate-bound
T6539 14100-14113 NN denotes anti-CD3/CD28
T6540 14114-14116 IN denotes in
T6541 14117-14120 DT denotes the
T6542 14121-14129 NN denotes presence
T6543 14130-14132 IN denotes of
T6544 14133-14138 NN denotes TGF-β
T6545 14139-14145 CC denotes and/or
T6546 14146-14150 NN denotes IL-4
T6547 14151-14154 CC denotes and
T6548 14155-14164 VB denotes harvested
T6549 14165-14170 IN denotes after
T6550 14171-14172 CD denotes 5
T6551 14173-14174 NN denotes d
T6552 14176-14180 NN denotes IL-4
T6553 14181-14192 RB denotes efficiently
T6554 14193-14202 VB denotes repressed
T6555 14203-14206 DT denotes the
T6556 14207-14221 JJ denotes TGF-β–mediated
T6557 14222-14231 NN denotes induction
T6558 14232-14234 IN denotes of
T6559 14235-14240 NN denotes FOXP3
T6560 14241-14251 NN denotes expression
T6561 14252-14253 -LRB- denotes (
T6562 14253-14259 NN denotes Figure
T6563 14260-14262 NN denotes 5A
T6564 14262-14263 -RRB- denotes )
T6565 14264-14266 IN denotes in
T6566 14267-14268 DT denotes a
T6567 14269-14292 JJ denotes concentration-dependent
T6568 14293-14299 NN denotes manner
T6569 14300-14301 -LRB- denotes (
T6570 14301-14307 NN denotes Figure
T6571 14308-14310 NN denotes 5B
T6572 14310-14311 -RRB- denotes )
T6573 14313-14316 JJ denotes Low
T6574 14317-14323 NN denotes levels
T6575 14324-14326 IN denotes of
T6576 14327-14332 NN denotes GATA3
T6577 14333-14337 VB denotes were
T6578 14338-14345 VB denotes induced
T6579 14346-14350 RB denotes also
T6580 14351-14353 IN denotes in
T6581 14354-14357 DT denotes the
T6582 14358-14365 NN denotes absence
T6583 14366-14368 IN denotes of
T6584 14369-14373 NN denotes IL-4
T6585 14373-14374 -COMMA- denotes ,
T6586 14375-14377 IN denotes as
T6587 14378-14380 PRP denotes it
T6588 14381-14384 VB denotes was
T6589 14385-14395 RB denotes previously
T6590 14396-14404 VB denotes observed
T6591 14405-14406 -LRB- denotes [
T6592 14406-14407 CD denotes 3
T6593 14407-14408 -RRB- denotes ]
T6594 14410-14417 RB denotes However
T6595 14417-14418 -COMMA- denotes ,
T6596 14419-14421 IN denotes at
T6597 14422-14425 JJ denotes low
T6598 14426-14439 NN denotes concentration
T6599 14439-14440 -COMMA- denotes ,
T6600 14441-14445 NN denotes IL-4
T6601 14446-14449 VB denotes was
T6602 14450-14454 JJ denotes able
T6603 14455-14457 TO denotes to
T6604 14458-14468 RB denotes marginally
T6605 14469-14475 VB denotes induce
T6606 14476-14481 NN denotes FOXP3
T6607 14482-14492 NN denotes expression
T6608 14494-14496 IN denotes Of
T6609 14497-14501 NN denotes note
T6610 14501-14502 -COMMA- denotes ,
T6611 14503-14508 NN denotes GATA3
T6612 14509-14512 VB denotes was
T6613 14513-14517 RB denotes also
T6614 14518-14525 VB denotes induced
T6615 14526-14528 IN denotes in
T6616 14529-14532 DT denotes the
T6617 14533-14541 NN denotes presence
T6618 14542-14544 IN denotes of
T6619 14545-14550 NN denotes TGF-β
T6620 14551-14553 IN denotes at
T6621 14554-14558 JJ denotes high
T6622 14559-14563 NN denotes IL-4
T6623 14564-14577 NN denotes concentration
T6624 14578-14579 -LRB- denotes (
T6625 14579-14585 NN denotes Figure
T6626 14586-14588 NN denotes 5A
T6627 14589-14592 CC denotes and
T6628 14593-14595 NN denotes 5B
T6629 14595-14596 -RRB- denotes )
T6630 14598-14601 DT denotes The
T6631 14602-14615 JJ denotes IL-4–mediated
T6632 14616-14626 NN denotes prevention
T6633 14627-14629 IN denotes of
T6634 14630-14635 NN denotes FOXP3
T6635 14636-14646 NN denotes expression
T6636 14647-14650 VB denotes was
T6637 14651-14654 RB denotes not
T6638 14655-14661 VB denotes caused
T6639 14662-14664 IN denotes by
T6640 14665-14678 NN denotes interferences
T6641 14679-14681 IN denotes of
T6642 14682-14685 DT denotes the
T6643 14686-14694 NN denotes receptor
T6644 14695-14704 NN denotes signaling
T6645 14704-14705 -COMMA- denotes ,
T6646 14706-14713 IN denotes because
T6647 14714-14717 DT denotes the
T6648 14718-14733 NN denotes phosphorylation
T6649 14734-14736 IN denotes of
T6650 14737-14742 NN denotes SMAD2
T6651 14743-14745 CC denotes or
T6652 14746-14751 NN denotes STAT6
T6653 14752-14755 VB denotes was
T6654 14756-14759 RB denotes not
T6655 14760-14768 VB denotes affected
T6656 14769-14771 IN denotes by
T6657 14772-14775 DT denotes the
T6658 14776-14784 NN denotes addition
T6659 14785-14787 IN denotes of
T6660 14788-14792 NN denotes IL-4
T6661 14793-14799 CC denotes and/or
T6662 14800-14805 NN denotes TGF-β
T6663 14805-14806 -COMMA- denotes ,
T6664 14807-14812 WDT denotes which
T6665 14813-14825 VB denotes demonstrates
T6666 14826-14830 IN denotes that
T6667 14831-14835 NN denotes IL-4
T6668 14836-14838 RB denotes as
T6669 14839-14843 RB denotes well
T6670 14844-14846 IN denotes as
T6671 14847-14852 NN denotes TGF-β
T6672 14853-14862 NN denotes signaling
T6673 14863-14867 VB denotes were
T6674 14868-14878 JJ denotes functional
T6675 14879-14884 IN denotes under
T6676 14885-14890 DT denotes these
T6677 14891-14901 NN denotes conditions
T6678 14902-14903 -LRB- denotes (
T6679 14903-14909 NN denotes Figure
T6680 14910-14912 NN denotes 5C
T6681 14912-14913 -RRB- denotes )
T6682 14915-14925 VB denotes Increasing
T6683 14926-14933 NN denotes amounts
T6684 14934-14936 IN denotes of
T6685 14937-14941 NN denotes IL-4
T6686 14942-14950 NN denotes increase
T6687 14951-14964 JJ denotes intracellular
T6688 14965-14970 NN denotes GATA3
T6689 14970-14971 -COMMA- denotes ,
T6690 14972-14979 IN denotes whereas
T6691 14980-14985 NN denotes FOXP3
T6692 14986-14995 VB denotes decreased
T6693 14995-14996 -COMMA- denotes ,
T6694 14997-15002 WDT denotes which
T6695 15003-15005 VB denotes is
T6696 15006-15016 JJ denotes consistent
T6697 15017-15021 IN denotes with
T6698 15022-15025 DT denotes the
T6699 15026-15030 NN denotes mRNA
T6700 15031-15039 NN denotes analysis
T6701 15040-15041 -LRB- denotes (
T6702 15041-15047 NN denotes Figure
T6703 15048-15050 NN denotes 5D
T6704 15050-15051 -RRB- denotes )
T6705 15053-15064 RB denotes Furthermore
T6706 15064-15065 -COMMA- denotes ,
T6707 15066-15075 NN denotes injection
T6708 15076-15078 IN denotes of
T6709 15079-15083 NN denotes IL-4
T6710 15084-15088 IN denotes into
T6711 15089-15098 JJ denotes wild-type
T6712 15099-15101 NN denotes B6
T6713 15102-15106 NN denotes mice
T6714 15107-15116 VB denotes decreased
T6715 15117-15120 DT denotes the
T6716 15121-15130 JJ denotes inducible
T6717 15131-15133 CC denotes or
T6718 15134-15141 JJ denotes natural
T6719 15142-15146 NN denotes Treg
T6720 15147-15153 NN denotes number
T6721 15154-15156 FW denotes in
T6722 15157-15161 FW denotes vivo
T6723 15163-15164 DT denotes A
T6724 15165-15176 NN denotes distinction
T6725 15177-15179 IN denotes of
T6726 15180-15183 DT denotes the
T6727 15184-15188 NN denotes Treg
T6728 15189-15196 NN denotes subsets
T6729 15197-15199 VB denotes is
T6730 15200-15203 RB denotes not
T6731 15204-15212 JJ denotes possible
T6732 15212-15213 -COMMA- denotes ,
T6733 15214-15221 IN denotes because
T6734 15222-15230 RB denotes recently
T6735 15231-15240 VB denotes activated
T6736 15241-15246 NN denotes iTreg
T6737 15247-15252 NN denotes cells
T6738 15253-15257 RB denotes also
T6739 15258-15265 VB denotes express
T6740 15266-15273 NN denotes surface
T6741 15274-15278 NN denotes CD25
T6742 15280-15282 PRP denotes We
T6743 15283-15287 VB denotes used
T6744 15288-15297 NN denotes complexes
T6745 15298-15300 IN denotes of
T6746 15301-15312 JJ denotes recombinant
T6747 15313-15318 NN denotes mouse
T6748 15319-15323 NN denotes IL-4
T6749 15324-15325 -LRB- denotes (
T6750 15325-15331 NN denotes rmIL-4
T6751 15331-15332 -RRB- denotes )
T6752 15333-15337 CC denotes plus
T6753 15338-15347 JJ denotes anti-IL-4
T6754 15348-15358 JJ denotes monoclonal
T6755 15359-15369 NN denotes antibodies
T6756 15370-15371 -LRB- denotes (
T6757 15371-15375 NN denotes mAbs
T6758 15375-15376 -RRB- denotes )
T6759 15376-15377 -COMMA- denotes ,
T6760 15378-15383 WDT denotes which
T6761 15384-15388 VB denotes have
T6762 15389-15393 VB denotes been
T6763 15394-15399 VB denotes shown
T6764 15400-15402 TO denotes to
T6765 15403-15415 RB denotes dramatically
T6766 15416-15424 VB denotes increase
T6767 15425-15428 DT denotes the
T6768 15429-15436 NN denotes potency
T6769 15437-15439 IN denotes of
T6770 15440-15443 DT denotes the
T6771 15444-15452 NN denotes cytokine
T6772 15453-15455 FW denotes in
T6773 15456-15460 FW denotes vivo
T6774 15461-15462 -LRB- denotes [
T6775 15462-15464 CD denotes 19
T6776 15464-15465 -RRB- denotes ]
T6777 15467-15469 IN denotes In
T6778 15470-15475 DT denotes these
T6779 15476-15480 NN denotes mice
T6780 15480-15481 -COMMA- denotes ,
T6781 15482-15485 DT denotes the
T6782 15486-15496 NN denotes percentage
T6783 15497-15499 IN denotes of
T6784 15500-15509 JJ denotes CD4+CD25+
T6785 15510-15513 CC denotes and
T6786 15514-15520 JJ denotes FOXP3+
T6787 15521-15522 NN denotes T
T6788 15523-15528 NN denotes cells
T6789 15529-15541 RB denotes dramatically
T6790 15542-15551 VB denotes decreased
T6791 15552-15556 WRB denotes when
T6792 15557-15560 DT denotes the
T6793 15561-15578 JJ denotes antibody-cytokine
T6794 15579-15585 JJ denotes immune
T6795 15586-15595 NN denotes complexes
T6796 15596-15600 VB denotes were
T6797 15601-15609 VB denotes injected
T6798 15610-15611 -LRB- denotes (
T6799 15611-15617 NN denotes Figure
T6800 15618-15620 NN denotes S2
T6801 15620-15621 -RRB- denotes )
T6802 15623-15627 IN denotes Upon
T6803 15628-15642 NN denotes administration
T6804 15643-15645 IN denotes of
T6805 15646-15652 NN denotes rmIL-4
T6806 15653-15657 CC denotes plus
T6807 15658-15667 JJ denotes anti-IL-4
T6808 15668-15671 NN denotes mAb
T6809 15672-15681 NN denotes complexes
T6810 15681-15682 -COMMA- denotes ,
T6811 15683-15686 DT denotes the
T6812 15687-15692 JJ denotes total
T6813 15693-15699 NN denotes number
T6814 15700-15702 IN denotes of
T6815 15703-15712 JJ denotes CD4+CD25+
T6816 15713-15714 NN denotes T
T6817 15715-15719 NN denotes cell
T6818 15719-15720 -COMMA- denotes ,
T6819 15721-15723 RB denotes as
T6820 15724-15728 RB denotes well
T6821 15729-15731 IN denotes as
T6822 15732-15735 DT denotes the
T6823 15736-15742 JJ denotes Foxp3+
T6824 15743-15744 NN denotes T
T6825 15745-15750 NN denotes cells
T6826 15751-15761 VB denotes diminished
T6827 15762-15764 IN denotes by
T6828 15765-15769 NN denotes half
T6829 15770-15771 -LRB- denotes (
T6830 15771-15777 NN denotes Figure
T6831 15778-15781 NN denotes S2G
T6832 15782-15785 CC denotes and
T6833 15786-15789 NN denotes S2H
T6834 15789-15790 -RRB- denotes )
T6835 15790-15791 -COMMA- denotes ,
T6836 15792-15802 VB denotes confirming
T6837 15803-15807 IN denotes that
T6838 15808-15811 DT denotes the
T6839 15812-15817 JJ denotes lower
T6840 15818-15828 NN denotes percentage
T6841 15829-15832 VB denotes was
T6842 15833-15836 RB denotes not
T6843 15837-15840 JJ denotes due
T6844 15841-15843 TO denotes to
T6845 15844-15846 DT denotes an
T6846 15847-15855 NN denotes increase
T6847 15856-15858 IN denotes in
T6848 15859-15862 DT denotes the
T6849 15863-15872 NN denotes CD4+CD25–
T6850 15873-15878 NN denotes cells
T6851 15878-15879 -COMMA- denotes ,
T6852 15880-15883 CC denotes but
T6853 15884-15885 DT denotes a
T6854 15886-15890 JJ denotes real
T6855 15891-15899 NN denotes decrease
T6856 15900-15902 IN denotes of
T6857 15903-15912 JJ denotes CD4+CD25+
T6858 15913-15914 NN denotes T
T6859 15915-15920 NN denotes cells
T6860 15922-15924 IN denotes In
T6861 15925-15935 NN denotes conclusion
T6862 15935-15936 -COMMA- denotes ,
T6863 15937-15941 NN denotes IL-4
T6864 15942-15952 RB denotes negatively
T6865 15953-15962 VB denotes regulates
T6866 15963-15966 DT denotes the
T6867 15967-15974 JJ denotes natural
T6868 15975-15977 CC denotes or
T6869 15978-15987 JJ denotes inducible
T6870 15988-15992 NN denotes Treg
T6871 15993-15997 NN denotes cell
T6872 15998-16006 NN denotes turnover
T6873 16007-16010 RB denotes not
T6874 16011-16015 RB denotes only
T6875 16016-16018 FW denotes in
T6876 16019-16024 FW denotes vitro
T6877 16025-16028 CC denotes but
T6878 16029-16033 RB denotes also
T6879 16034-16036 FW denotes in
T6880 16037-16041 FW denotes vivo
T6881 16043-16045 TO denotes To
T6882 16046-16051 VB denotes study
T6883 16052-16055 DT denotes the
T6884 16056-16063 NN denotes effects
T6885 16064-16066 IN denotes of
T6886 16067-16071 NN denotes IL-4
T6887 16072-16074 IN denotes on
T6888 16075-16091 JJ denotes already-existing
T6889 16092-16097 JJ denotes human
T6890 16098-16105 JJ denotes natural
T6891 16106-16108 CC denotes or
T6892 16109-16118 JJ denotes inducible
T6893 16119-16123 NN denotes Treg
T6894 16124-16129 NN denotes cells
T6895 16129-16130 -COMMA- denotes ,
T6896 16131-16133 PRP denotes we
T6897 16134-16141 VB denotes exposed
T6898 16142-16148 VB denotes sorted
T6899 16149-16154 JJ denotes CD25+
T6900 16155-16156 NN denotes T
T6901 16157-16162 NN denotes cells
T6902 16163-16164 -LRB- denotes (
T6903 16164-16169 NN denotes nTreg
T6904 16170-16175 NN denotes cells
T6905 16175-16176 -RRB- denotes )
T6906 16177-16179 TO denotes to
T6907 16180-16184 NN denotes IL-4
T6908 16185-16188 CC denotes and
T6909 16189-16197 VB denotes analyzed
T6910 16198-16203 NN denotes FOXP3
T6911 16204-16214 NN denotes expression
T6912 16215-16218 CC denotes and
T6913 16219-16230 JJ denotes suppressive
T6914 16231-16239 NN denotes capacity
T6915 16241-16243 IN denotes In
T6916 16244-16260 JJ denotes already-existing
T6917 16261-16265 NN denotes Treg
T6918 16266-16271 NN denotes cells
T6919 16271-16272 -COMMA- denotes ,
T6920 16273-16277 NN denotes IL-4
T6921 16278-16284 VB denotes failed
T6922 16285-16287 TO denotes to
T6923 16288-16295 VB denotes inhibit
T6924 16296-16301 NN denotes FOXP3
T6925 16302-16312 NN denotes expression
T6926 16313-16314 -LRB- denotes (
T6927 16314-16320 NN denotes Figure
T6928 16321-16324 NN denotes S3A
T6929 16324-16325 -RRB- denotes )
T6930 16325-16326 -COMMA- denotes ,
T6931 16327-16330 CC denotes and
T6932 16331-16334 DT denotes the
T6933 16335-16346 JJ denotes suppressive
T6934 16347-16355 NN denotes capacity
T6935 16356-16359 VB denotes was
T6936 16360-16363 RB denotes not
T6937 16364-16371 VB denotes altered
T6938 16372-16373 -LRB- denotes (
T6939 16373-16379 NN denotes Figure
T6940 16380-16383 NN denotes S3C
T6941 16383-16384 -RRB- denotes )
T6942 16386-16395 RB denotes Similarly
T6943 16396-16408 JJ denotes pre-existing
T6944 16409-16414 NN denotes iTreg
T6945 16415-16420 NN denotes cells
T6946 16421-16424 VB denotes did
T6947 16425-16428 RB denotes not
T6948 16429-16437 VB denotes decrease
T6949 16438-16443 NN denotes FOXP3
T6950 16444-16454 NN denotes expression
T6951 16455-16459 IN denotes upon
T6952 16460-16464 NN denotes IL-4
T6953 16465-16473 NN denotes exposure
T6954 16474-16475 -LRB- denotes (
T6955 16475-16481 NN denotes Figure
T6956 16482-16485 NN denotes S3B
T6957 16485-16486 -RRB- denotes )
T8352 17545-17548 DT denotes The
T8353 17549-17553 NN denotes Role
T8354 17554-17556 IN denotes of
T8355 17557-17562 NN denotes TGF-β
T8356 17563-17565 IN denotes in
T8357 17566-17567 NN denotes T
T8358 17568-17572 NN denotes Cell
T8359 17573-17588 NN denotes Differentiation
T8360 17589-17597 IN denotes Although
T8361 17598-17611 JJ denotes TGF-β–reduced
T8362 17612-17616 NN denotes CD25
T8363 17616-17617 -COMMA- denotes ,
T8364 17618-17622 NN denotes IL-4
T8365 17623-17633 NN denotes expression
T8366 17633-17634 -COMMA- denotes ,
T8367 17635-17638 CC denotes and
T8368 17639-17643 NN denotes CD25
T8369 17644-17654 NN denotes expression
T8370 17655-17656 -LRB- denotes (
T8371 17656-17662 NN denotes Figure
T8372 17663-17665 NN denotes 6B
T8373 17665-17666 -RRB- denotes )
T8374 17666-17667 -COMMA- denotes ,
T8375 17668-17672 NN denotes IL-4
T8376 17673-17686 RB denotes significantly
T8377 17687-17696 VB denotes inhibited
T8378 17697-17711 JJ denotes TGF-β–mediated
T8379 17712-17721 NN denotes induction
T8380 17722-17724 IN denotes of
T8381 17725-17730 NN denotes FOXP3
T8382 17731-17733 IN denotes in
T8383 17734-17739 JJ denotes naive
T8384 17740-17741 NN denotes T
T8385 17742-17747 NN denotes cells
T8386 17748-17754 VB denotes driven
T8387 17755-17761 IN denotes toward
T8388 17762-17768 JJ denotes FOXP3+
T8389 17769-17770 NN denotes T
T8390 17771-17776 NN denotes cells
T8391 17776-17777 -COMMA- denotes ,
T8392 17778-17780 IN denotes as
T8393 17781-17786 VB denotes shown
T8394 17787-17789 IN denotes by
T8395 17790-17794 NN denotes FACS
T8396 17795-17803 NN denotes analysis
T8397 17804-17805 -LRB- denotes (
T8398 17805-17811 NN denotes Figure
T8399 17812-17814 NN denotes 6A
T8400 17814-17815 -RRB- denotes )
T8401 17817-17819 PRP denotes It
T8402 17820-17822 VB denotes is
T8403 17823-17828 VB denotes known
T8404 17829-17833 IN denotes that
T8405 17834-17838 NN denotes IL-4
T8406 17839-17841 VB denotes is
T8407 17842-17843 DT denotes a
T8408 17844-17850 JJ denotes potent
T8409 17851-17857 NN denotes growth
T8410 17858-17864 NN denotes factor
T8411 17865-17868 CC denotes and
T8412 17869-17872 MD denotes may
T8413 17873-17882 RB denotes therefore
T8414 17883-17888 VB denotes favor
T8415 17889-17892 DT denotes the
T8416 17893-17906 NN denotes proliferation
T8417 17907-17909 IN denotes of
T8418 17910-17916 NN denotes FOXP3–
T8419 17917-17922 NN denotes cells
T8420 17923-17926 CC denotes and
T8421 17927-17931 RB denotes thus
T8422 17932-17940 VB denotes decrease
T8423 17941-17944 DT denotes the
T8424 17945-17953 JJ denotes relative
T8425 17954-17964 NN denotes percentage
T8426 17965-17967 IN denotes of
T8427 17968-17974 JJ denotes FOXP3+
T8428 17975-17980 NN denotes cells
T8429 17982-17989 RB denotes However
T8430 17989-17990 -COMMA- denotes ,
T8431 17991-17999 NN denotes analysis
T8432 18000-18002 IN denotes of
T8433 18003-18007 NN denotes cell
T8434 18008-18016 NN denotes division
T8435 18017-18025 NN denotes kinetics
T8436 18026-18028 IN denotes by
T8437 18029-18042 NN denotes CFSE-labeling
T8438 18043-18055 VB denotes demonstrated
T8439 18056-18060 IN denotes that
T8440 18061-18065 NN denotes IL-4
T8441 18066-18069 VB denotes did
T8442 18070-18073 RB denotes not
T8443 18074-18088 RB denotes differentially
T8444 18089-18096 VB denotes promote
T8445 18097-18101 NN denotes cell
T8446 18102-18108 NN denotes growth
T8447 18109-18111 IN denotes of
T8448 18112-18118 NN denotes FOXP3+
T8449 18119-18123 IN denotes over
T8450 18124-18128 DT denotes that
T8451 18129-18131 IN denotes of
T8452 18132-18138 NN denotes FOXP3–
T8453 18140-18142 IN denotes In
T8454 18143-18147 NN denotes fact
T8455 18148-18152 DT denotes both
T8456 18153-18164 NN denotes populations
T8457 18165-18171 VB denotes showed
T8458 18172-18181 RB denotes similarly
T8459 18182-18190 VB denotes enhanced
T8460 18191-18204 NN denotes proliferation
T8461 18205-18206 -LRB- denotes (
T8462 18206-18212 NN denotes Figure
T8463 18213-18215 NN denotes 6A
T8464 18215-18216 -RRB- denotes )
T8465 18218-18229 RB denotes Furthermore
T8466 18230-18233 DT denotes the
T8467 18234-18248 JJ denotes TGF-β–mediated
T8468 18249-18258 NN denotes induction
T8469 18259-18261 IN denotes of
T8470 18262-18267 NN denotes FOXP3
T8471 18268-18278 NN denotes expression
T8472 18279-18282 VB denotes was
T8473 18283-18286 RB denotes not
T8474 18287-18293 VB denotes caused
T8475 18294-18296 IN denotes by
T8476 18297-18307 NN denotes overgrowth
T8477 18308-18310 IN denotes of
T8478 18311-18312 DT denotes a
T8479 18313-18324 JJ denotes CD25–FOXP3+
T8480 18325-18333 NN denotes minority
T8481 18333-18334 -COMMA- denotes ,
T8482 18335-18340 IN denotes since
T8483 18341-18344 DT denotes the
T8484 18345-18351 NN denotes number
T8485 18352-18354 IN denotes of
T8486 18355-18361 JJ denotes FOXP3+
T8487 18362-18367 NN denotes cells
T8488 18368-18371 VB denotes was
T8489 18372-18382 JJ denotes low/absent
T8490 18383-18385 IN denotes in
T8491 18386-18389 DT denotes the
T8492 18390-18398 VB denotes purified
T8493 18399-18410 JJ denotes CD4+CD45RA+
T8494 18411-18412 NN denotes T
T8495 18413-18418 NN denotes cells
T8496 18419-18420 -LRB- denotes (
T8497 18420-18427 IN denotes between
T8498 18428-18429 CD denotes 0
T8499 18429-18430 NN denotes %
T8500 18431-18434 CC denotes and
T8501 18435-18436 CD denotes 1
T8502 18436-18437 NN denotes %
T8503 18437-18438 -RRB- denotes )
T8504 18438-18439 -COMMA- denotes ,
T8505 18440-18443 CC denotes and
T8506 18444-18447 DT denotes the
T8507 18448-18454 JJ denotes FOXP3+
T8508 18455-18460 NN denotes cells
T8509 18461-18465 VB denotes were
T8510 18466-18469 RB denotes not
T8511 18470-18478 VB denotes confined
T8512 18479-18481 TO denotes to
T8513 18482-18485 DT denotes the
T8514 18486-18492 RB denotes highly
T8515 18493-18500 VB denotes divided
T8516 18501-18506 NN denotes cells
T8517 18508-18512 NN denotes CD25
T8518 18513-18516 VB denotes was
T8519 18517-18531 VB denotes down-regulated
T8520 18532-18534 IN denotes in
T8521 18535-18548 JJ denotes TGF-β–treated
T8522 18549-18554 NN denotes cells
T8523 18555-18562 VB denotes compare
T8524 18563-18565 TO denotes to
T8525 18566-18575 VB denotes activated
T8526 18576-18577 NN denotes T
T8527 18578-18583 NN denotes cells
T8528 18583-18584 -COMMA- denotes ,
T8529 18585-18590 WDT denotes which
T8530 18591-18594 VB denotes was
T8531 18595-18599 RB denotes even
T8532 18600-18604 RB denotes more
T8533 18605-18615 JJ denotes pronounced
T8534 18616-18618 IN denotes in
T8535 18619-18624 NN denotes cells
T8536 18625-18633 VB denotes cultured
T8537 18634-18638 IN denotes with
T8538 18639-18644 NN denotes TGF-β
T8539 18645-18648 CC denotes and
T8540 18649-18653 NN denotes IL-4
T8541 19280-19283 DT denotes The
T8542 19284-19292 NN denotes addition
T8543 19293-19295 IN denotes of
T8544 19296-19300 NN denotes IL-4
T8545 19301-19303 TO denotes to
T8546 19304-19317 JJ denotes iTreg-driving
T8547 19318-19328 NN denotes conditions
T8548 19329-19338 VB denotes decreased
T8549 19339-19342 DT denotes the
T8550 19343-19349 NN denotes number
T8551 19350-19352 IN denotes of
T8552 19353-19359 JJ denotes FOXP3+
T8553 19360-19365 NN denotes cells
T8554 19366-19367 -LRB- denotes (
T8555 19367-19373 NN denotes Figure
T8556 19374-19376 NN denotes 6B
T8557 19376-19377 -RRB- denotes )
T8558 19379-19381 IN denotes In
T8559 19382-19386 NN denotes line
T8560 19387-19391 IN denotes with
T8561 19392-19395 DT denotes the
T8562 19396-19404 JJ denotes previous
T8563 19405-19413 NN denotes findings
T8564 19413-19414 -COMMA- denotes ,
T8565 19415-19418 DT denotes the
T8566 19419-19433 JJ denotes IL-4–producing
T8567 19434-19439 NN denotes cells
T8568 19440-19443 CC denotes and
T8569 19444-19447 DT denotes the
T8570 19448-19453 NN denotes FOXP3
T8571 19454-19464 NN denotes expressing
T8572 19465-19470 NN denotes cells
T8573 19471-19474 VB denotes are
T8574 19475-19489 JJ denotes nonoverlapping
T8575 19490-19501 NN denotes populations
T8576 19503-19508 IN denotes Since
T8577 19509-19514 NN denotes FOXP3
T8578 19515-19517 VB denotes is
T8579 19518-19523 VB denotes known
T8580 19524-19526 TO denotes to
T8581 19527-19530 VB denotes act
T8582 19531-19533 IN denotes as
T8583 19534-19535 DT denotes a
T8584 19536-19545 NN denotes repressor
T8585 19546-19548 IN denotes of
T8586 19549-19557 NN denotes cytokine
T8587 19558-19568 NN denotes expression
T8588 19569-19570 -LRB- denotes [
T8589 19570-19572 CD denotes 20
T8590 19572-19573 -RRB- denotes ]
T8591 19573-19574 -COMMA- denotes ,
T8592 19575-19577 PRP denotes we
T8593 19578-19587 RB denotes therefore
T8594 19588-19596 VB denotes analyzed
T8595 19597-19602 NN denotes GATA3
T8596 19603-19606 CC denotes and
T8597 19607-19612 NN denotes FOXP3
T8598 19613-19623 NN denotes expression
T8599 19625-19628 DT denotes The
T8600 19629-19639 NN denotes expression
T8601 19640-19647 JJ denotes kinetic
T8602 19648-19650 IN denotes of
T8603 19651-19656 JJ denotes naive
T8604 19657-19658 NN denotes T
T8605 19659-19664 NN denotes cells
T8606 19665-19672 VB denotes exposed
T8607 19673-19675 TO denotes to
T8608 19676-19680 NN denotes IL-4
T8609 19681-19684 CC denotes and
T8610 19685-19690 NN denotes TGF-β
T8611 19691-19703 VB denotes demonstrated
T8612 19704-19708 IN denotes that
T8613 19709-19714 NN denotes GATA3
T8614 19715-19718 CC denotes and
T8615 19719-19724 NN denotes FOXP3
T8616 19725-19728 VB denotes are
T8617 19729-19738 RB denotes initially
T8618 19739-19744 VB denotes found
T8619 19745-19747 IN denotes in
T8620 19748-19756 JJ denotes separate
T8621 19757-19768 NN denotes populations
T8622 19769-19770 -LRB- denotes (
T8623 19770-19773 NN denotes day
T8624 19774-19775 CD denotes 2
T8625 19775-19776 -RRB- denotes )
T8626 19776-19777 -COMMA- denotes ,
T8627 19778-19781 CC denotes but
T8628 19782-19793 RB denotes transiently
T8629 19794-19804 VB denotes co-express
T8630 19805-19809 DT denotes both
T8631 19810-19817 NN denotes factors
T8632 19818-19819 -LRB- denotes (
T8633 19819-19823 NN denotes days
T8634 19824-19827 CD denotes 4–8
T8635 19827-19828 -RRB- denotes )
T8636 19828-19829 -COMMA- denotes ,
T8637 19830-19836 IN denotes before
T8638 19837-19849 VB denotes establishing
T8639 19850-19858 JJ denotes separate
T8640 19859-19870 NN denotes populations
T8641 19871-19873 IN denotes at
T8642 19874-19877 DT denotes the
T8643 19878-19881 NN denotes end
T8644 19882-19884 IN denotes of
T8645 19885-19888 DT denotes the
T8646 19889-19904 NN denotes differentiation
T8647 19905-19912 NN denotes process
T8648 19913-19914 -LRB- denotes (
T8649 19914-19917 NN denotes day
T8650 19918-19920 CD denotes 10
T8651 19920-19921 -COLON- denotes ;
T8652 19922-19928 NN denotes Figure
T8653 19929-19931 NN denotes 6C
T8654 19931-19932 -RRB- denotes )
T8655 19932-19933 -COMMA- denotes ,
T8656 19934-19944 VB denotes suggesting
T8657 19945-19949 IN denotes that
T8658 19950-19955 NN denotes GATA3
T8659 19956-19964 VB denotes inhibits
T8660 19965-19968 DT denotes the
T8661 19969-19980 NN denotes development
T8662 19981-19983 IN denotes of
T8663 19984-19989 NN denotes iTreg
T8664 19990-19995 NN denotes cells
T8665 19996-19998 IN denotes by
T8666 19999-20009 VB denotes repressing
T8667 20010-20015 NN denotes FOXP3
T8668 20017-20022 DT denotes These
T8669 20023-20030 NN denotes results
T8670 20031-20037 VB denotes showed
T8671 20038-20042 IN denotes that
T8672 20043-20047 NN denotes IL-4
T8673 20048-20052 VB denotes acts
T8674 20053-20055 FW denotes in
T8675 20056-20061 FW denotes vitro
T8676 20062-20064 IN denotes as
T8677 20065-20067 DT denotes an
T8678 20068-20077 NN denotes inhibitor
T8679 20078-20080 IN denotes of
T8680 20081-20086 NN denotes FOXP3
T8681 20087-20097 NN denotes expression
T8682 20097-20098 -COMMA- denotes ,
T8683 20099-20106 IN denotes without
T8684 20107-20118 VB denotes interfering
T8685 20119-20123 IN denotes with
T8686 20124-20129 NN denotes TGF-β
T8687 20130-20139 NN denotes signaling
T8688 20139-20140 -COMMA- denotes ,
T8689 20141-20149 RB denotes probably
T8690 20150-20156 VB denotes acting
T8691 20157-20159 IN denotes at
T8692 20160-20163 DT denotes the
T8693 20164-20169 NN denotes level
T8694 20170-20172 IN denotes of
T8695 20173-20186 NN denotes transcription
T8696 20187-20194 NN denotes factors
T8697 20194-20195 -COMMA- denotes ,
T8698 20196-20199 CC denotes and
T8699 20200-20208 RB denotes possibly
T8700 20209-20211 IN denotes by
T8701 20212-20213 DT denotes a
T8702 20214-20229 JJ denotes GATA3-dependent
T8703 20230-20239 NN denotes mechanism
T9866 20242-20247 NN denotes GATA3
T9867 20248-20250 VB denotes Is
T9868 20251-20252 DT denotes a
T9869 20253-20261 JJ denotes Negative
T9870 20262-20271 NN denotes Regulator
T9871 20272-20274 IN denotes of
T9872 20275-20280 NN denotes FOXP3
T9873 20281-20291 NN denotes Expression
T9874 20292-20297 NN denotes FOXP3
T9875 20298-20308 NN denotes expression
T9876 20309-20318 VB denotes decreased
T9877 20319-20323 RB denotes once
T9878 20324-20329 NN denotes GATA3
T9879 20330-20340 NN denotes expression
T9880 20341-20343 VB denotes is
T9881 20344-20348 JJ denotes high
T9882 20348-20349 -COLON- denotes ;
T9883 20350-20359 RB denotes therefore
T9884 20359-20360 -COMMA- denotes ,
T9885 20361-20363 PRP denotes we
T9886 20364-20376 VB denotes hypothesized
T9887 20377-20378 DT denotes a
T9888 20379-20388 JJ denotes potential
T9889 20389-20393 NN denotes role
T9890 20394-20397 IN denotes for
T9891 20398-20403 NN denotes GATA3
T9892 20404-20406 IN denotes in
T9893 20407-20417 VB denotes repressing
T9894 20418-20423 NN denotes FOXP3
T9895 20425-20432 IN denotes Besides
T9896 20433-20440 NN denotes GATA3′s
T9897 20441-20451 JJ denotes well-known
T9898 20452-20460 JJ denotes positive
T9899 20461-20467 NN denotes effect
T9900 20468-20470 IN denotes on
T9901 20471-20475 NN denotes gene
T9902 20476-20486 NN denotes regulation
T9903 20486-20487 -COMMA- denotes ,
T9904 20488-20495 NN denotes GATA3′s
T9905 20496-20506 JJ denotes repressive
T9906 20507-20519 NN denotes capabilities
T9907 20520-20524 VB denotes were
T9908 20525-20535 RB denotes previously
T9909 20536-20541 VB denotes shown
T9910 20542-20544 TO denotes to
T9911 20545-20553 VB denotes restrict
T9912 20554-20557 NN denotes Th1
T9913 20558-20568 NN denotes commitment
T9914 20569-20571 IN denotes by
T9915 20572-20582 VB denotes inhibiting
T9916 20583-20588 NN denotes STAT4
T9917 20589-20599 NN denotes expression
T9918 20600-20601 -LRB- denotes [
T9919 20601-20605 CD denotes 2,21
T9920 20605-20606 -RRB- denotes ]
T9921 20606-20607 -COMMA- denotes ,
T9922 20608-20611 CC denotes and
T9923 20612-20621 RB denotes therefore
T9924 20622-20627 NN denotes GATA3
T9925 20628-20636 VB denotes prevents
T9926 20637-20652 NN denotes differentiation
T9927 20653-20657 IN denotes into
T9928 20658-20661 NN denotes Th1
T9929 20662-20667 NN denotes cells
T9930 20669-20671 TO denotes To
T9931 20672-20683 VB denotes investigate
T9932 20684-20691 IN denotes whether
T9933 20692-20697 NN denotes GATA3
T9934 20698-20701 MD denotes can
T9935 20702-20710 RB denotes directly
T9936 20711-20718 VB denotes inhibit
T9937 20719-20724 NN denotes FOXP3
T9938 20725-20734 NN denotes induction
T9939 20734-20735 -COMMA- denotes ,
T9940 20736-20738 PRP denotes we
T9941 20739-20749 VB denotes transduced
T9942 20750-20755 NN denotes GATA3
T9943 20756-20758 CC denotes or
T9944 20759-20760 DT denotes a
T9945 20761-20770 VB denotes truncated
T9946 20771-20776 NN denotes GATA3
T9947 20777-20784 VB denotes lacking
T9948 20785-20788 DT denotes the
T9949 20789-20800 JJ denotes DNA-binding
T9950 20801-20807 NN denotes domain
T9951 20808-20810 IN denotes in
T9952 20811-20816 JJ denotes human
T9953 20817-20824 JJ denotes primary
T9954 20825-20836 JJ denotes CD4+CD45RA+
T9955 20837-20838 NN denotes T
T9956 20839-20844 NN denotes cells
T9957 20845-20850 VB denotes using
T9958 20851-20852 DT denotes a
T9959 20853-20862 JJ denotes TAT-fused
T9960 20862-20863 -COMMA- denotes ,
T9961 20864-20877 RB denotes recombinantly
T9962 20878-20887 VB denotes expressed
T9963 20888-20893 NN denotes GATA3
T9964 20895-20900 IN denotes After
T9965 20901-20913 NN denotes transduction
T9966 20913-20914 -COMMA- denotes ,
T9967 20915-20918 DT denotes the
T9968 20919-20924 NN denotes cells
T9969 20925-20929 VB denotes were
T9970 20930-20939 VB denotes activated
T9971 20940-20944 IN denotes with
T9972 20945-20952 JJ denotes soluble
T9973 20953-20966 NN denotes anti-CD3/CD28
T9974 20967-20969 IN denotes in
T9975 20970-20973 DT denotes the
T9976 20974-20982 NN denotes presence
T9977 20983-20985 CC denotes or
T9978 20986-20993 NN denotes absence
T9979 20994-20996 IN denotes of
T9980 20997-21002 NN denotes TGF-β
T9981 21004-21013 NN denotes TAT-GATA3
T9982 21014-21017 VB denotes was
T9983 21018-21030 RB denotes successfully
T9984 21031-21041 VB denotes transduced
T9985 21042-21044 IN denotes in
T9986 21045-21046 DT denotes a
T9987 21047-21058 JJ denotes homogeneous
T9988 21059-21062 CC denotes and
T9989 21063-21077 JJ denotes dose-dependent
T9990 21078-21084 NN denotes manner
T9991 21085-21089 IN denotes into
T9992 21090-21095 JJ denotes human
T9993 21096-21100 JJ denotes CD4+
T9994 21101-21102 NN denotes T
T9995 21103-21108 NN denotes cells
T9996 21109-21110 -LRB- denotes (
T9997 21110-21116 NNP denotes Figure
T9998 21117-21119 NNP denotes 7A
T9999 21119-21120 -COMMA- denotes ,
T10000 21121-21126 JJ denotes upper
T10001 21127-21132 NN denotes panel
T10002 21132-21133 -RRB- denotes )
T10003 21135-21144 NN denotes TAT-GATA3
T10004 21145-21152 VB denotes reduced
T10005 21153-21158 NN denotes FOXP3
T10006 21159-21169 NN denotes expression
T10007 21170-21172 IN denotes in
T10008 21173-21174 DT denotes a
T10009 21175-21189 JJ denotes dose-dependent
T10010 21190-21196 NN denotes manner
T10011 21196-21197 -COMMA- denotes ,
T10012 21198-21205 IN denotes whereas
T10013 21206-21207 DT denotes a
T10014 21208-21219 JJ denotes DNA-binding
T10015 21220-21226 NN denotes domain
T10016 21227-21236 VB denotes truncated
T10017 21237-21244 NN denotes version
T10018 21245-21246 -LRB- denotes (
T10019 21246-21260 NN denotes TAT-ΔDBD-GATA3
T10020 21260-21261 -RRB- denotes )
T10021 21262-21265 VB denotes did
T10022 21266-21269 RB denotes not
T10023 21270-21276 VB denotes affect
T10024 21277-21282 NN denotes FOXP3
T10025 21283-21293 NN denotes expression
T10026 21294-21296 IN denotes as
T10027 21297-21305 VB denotes compared
T10028 21306-21310 IN denotes with
T10029 21311-21321 NN denotes expression
T10030 21322-21324 IN denotes in
T10031 21325-21337 JJ denotes untransduced
T10032 21338-21343 NN denotes cells
T10033 21344-21345 -LRB- denotes (
T10034 21345-21351 NN denotes Figure
T10035 21352-21354 NN denotes 7A
T10036 21354-21355 -RRB- denotes )
T10037 21357-21359 IN denotes In
T10038 21360-21368 NN denotes addition
T10039 21368-21369 -COMMA- denotes ,
T10040 21370-21372 PRP denotes we
T10041 21373-21381 VB denotes analyzed
T10042 21382-21385 DT denotes the
T10043 21386-21396 JJ denotes inhibitory
T10044 21397-21403 NN denotes effect
T10045 21404-21406 IN denotes of
T10046 21407-21412 NN denotes GATA3
T10047 21413-21415 IN denotes on
T10048 21416-21421 NN denotes FOXP3
T10049 21422-21424 IN denotes in
T10050 21425-21435 JJ denotes transgenic
T10051 21436-21443 NN denotes DO11.10
T10052 21444-21448 NN denotes mice
T10053 21448-21449 -COMMA- denotes ,
T10054 21450-21464 RB denotes constitutively
T10055 21465-21479 VB denotes overexpressing
T10056 21480-21485 NN denotes GATA3
T10057 21486-21491 IN denotes under
T10058 21492-21495 DT denotes the
T10059 21496-21503 NN denotes control
T10060 21504-21506 IN denotes of
T10061 21507-21510 DT denotes the
T10062 21511-21514 NN denotes CD2
T10063 21515-21520 NN denotes locus
T10064 21521-21528 NN denotes control
T10065 21529-21535 NN denotes region
T10066 21536-21537 -LRB- denotes (
T10067 21537-21554 NN denotes DO11.10xCD2-GATA3
T10068 21554-21555 -RRB- denotes )
T10069 21557-21560 DT denotes The
T10070 21561-21567 JJ denotes thymic
T10071 21568-21577 NN denotes selection
T10072 21578-21582 IN denotes into
T10073 21583-21586 DT denotes the
T10074 21587-21590 NN denotes CD4
T10075 21591-21598 NN denotes lineage
T10076 21599-21601 VB denotes is
T10077 21602-21609 RB denotes largely
T10078 21610-21616 JJ denotes intact
T10079 21617-21619 IN denotes in
T10080 21620-21637 NN denotes DO11.10xCD2-GATA3
T10081 21638-21639 -LRB- denotes (
T10082 21639-21641 NNP denotes RW
T10083 21642-21650 NNP denotes Hendriks
T10084 21650-21651 -COMMA- denotes ,
T10085 21652-21663 JJ denotes unpublished
T10086 21664-21668 NN denotes data
T10087 21668-21669 -RRB- denotes )
T10088 21671-21676 DT denotes These
T10089 21677-21681 NN denotes mice
T10090 21682-21689 VB denotes develop
T10091 21690-21699 NN denotes lymphomas
T10092 21700-21702 IN denotes at
T10093 21703-21705 DT denotes an
T10094 21706-21711 JJ denotes older
T10095 21712-21715 NN denotes age
T10096 21715-21716 -COMMA- denotes ,
T10097 21717-21720 CC denotes but
T10098 21721-21726 NN denotes signs
T10099 21727-21729 IN denotes of
T10100 21730-21740 JJ denotes autoimmune
T10101 21741-21748 NN denotes disease
T10102 21749-21753 VB denotes were
T10103 21754-21757 RB denotes not
T10104 21758-21767 VB denotes described
T10105 21768-21769 -LRB- denotes [
T10106 21769-21771 CD denotes 22
T10107 21771-21772 -RRB- denotes ]
T10108 21774-21776 TO denotes To
T10109 21777-21788 VB denotes investigate
T10110 21789-21792 DT denotes the
T10111 21793-21799 NN denotes effect
T10112 21800-21802 IN denotes of
T10113 21803-21808 NN denotes GATA3
T10114 21809-21811 IN denotes on
T10115 21812-21817 NN denotes iTreg
T10116 21817-21818 -COMMA- denotes ,
T10117 21819-21834 NN denotes CD4+CD62L+CD25–
T10118 21835-21840 NN denotes cells
T10119 21841-21845 VB denotes were
T10120 21846-21854 VB denotes isolated
T10121 21854-21855 -COMMA- denotes ,
T10122 21856-21865 VB denotes activated
T10123 21866-21870 IN denotes with
T10124 21871-21874 NN denotes OVA
T10125 21875-21877 IN denotes in
T10126 21878-21881 DT denotes the
T10127 21882-21890 NN denotes presence
T10128 21891-21893 CC denotes or
T10129 21894-21901 NN denotes absence
T10130 21902-21904 IN denotes of
T10131 21905-21910 NN denotes TGF-β
T10132 21910-21911 -COMMA- denotes ,
T10133 21912-21915 CC denotes and
T10134 21916-21921 NN denotes Foxp3
T10135 21922-21932 NN denotes expression
T10136 21933-21936 VB denotes was
T10137 21937-21945 VB denotes analyzed
T10138 21946-21951 IN denotes after
T10139 21952-21953 CD denotes 4
T10140 21954-21955 NN denotes d
T10141 21957-21960 DT denotes The
T10142 21961-21966 JJ denotes naive
T10143 21967-21976 NN denotes CD4+CD25–
T10144 21977-21982 NN denotes cells
T10145 21983-21987 VB denotes were
T10146 21988-21994 CD denotes Foxp3–
T10147 21995-21996 -LRB- denotes (
T10148 21996-22007 JJ denotes unpublished
T10149 22008-22012 NN denotes data
T10150 22012-22013 -RRB- denotes )
T10151 22015-22017 IN denotes As
T10152 22018-22027 VB denotes described
T10153 22028-22031 IN denotes for
T10154 22032-22035 DT denotes the
T10155 22036-22041 JJ denotes human
T10156 22042-22047 NN denotes cells
T10157 22047-22048 -COMMA- denotes ,
T10158 22049-22054 NN denotes TGF-β
T10159 22055-22067 RB denotes dramatically
T10160 22068-22080 VB denotes up-regulated
T10161 22081-22086 NN denotes Foxp3
T10162 22087-22089 IN denotes in
T10163 22090-22093 DT denotes the
T10164 22094-22101 NN denotes DO11.10
T10165 22102-22112 NN denotes littermate
T10166 22113-22120 NN denotes control
T10167 22121-22125 NN denotes mice
T10168 22127-22129 IN denotes In
T10169 22130-22138 NN denotes contrast
T10170 22138-22139 -COMMA- denotes ,
T10171 22140-22145 NN denotes cells
T10172 22146-22150 IN denotes from
T10173 22151-22154 DT denotes the
T10174 22155-22172 NN denotes CD2-GATA3xDO11.10
T10175 22173-22177 NN denotes mice
T10176 22178-22184 VB denotes showed
T10177 22185-22197 RB denotes dramatically
T10178 22198-22205 VB denotes reduced
T10179 22206-22211 NN denotes Foxp3
T10180 22212-22222 NN denotes expression
T10181 22223-22227 WRB denotes when
T10182 22228-22237 VB denotes activated
T10183 22238-22242 IN denotes with
T10184 22243-22248 NN denotes TGF-β
T10185 22249-22252 CC denotes and
T10186 22253-22256 NN denotes OVA
T10187 22257-22258 -LRB- denotes (
T10188 22258-22264 NN denotes Figure
T10189 22265-22267 NN denotes 7B
T10190 22267-22268 -RRB- denotes )
T10191 22270-22273 DT denotes All
T10192 22274-22278 NN denotes mice
T10193 22279-22287 VB denotes produced
T10194 22288-22295 JJ denotes similar
T10195 22296-22303 NN denotes amounts
T10196 22304-22306 IN denotes of
T10197 22307-22312 NN denotes TGF-β
T10198 22312-22313 -COLON- denotes ;
T10199 22314-22316 IN denotes in
T10200 22317-22325 NN denotes addition
T10201 22325-22326 -COMMA- denotes ,
T10202 22327-22332 NN denotes Smad7
T10203 22333-22336 VB denotes was
T10204 22337-22344 RB denotes equally
T10205 22345-22354 VB denotes expressed
T10206 22355-22356 -LRB- denotes [
T10207 22356-22358 CD denotes 23
T10208 22358-22359 -RRB- denotes ]
T10209 22360-22362 IN denotes in
T10210 22363-22364 NN denotes T
T10211 22365-22370 NN denotes cells
T10212 22371-22373 IN denotes of
T10213 22374-22378 DT denotes both
T10214 22379-22383 NN denotes mice
T10215 22384-22391 NN denotes strains
T10216 22392-22393 -LRB- denotes (
T10217 22393-22399 NN denotes Figure
T10218 22400-22402 NN denotes 7C
T10219 22402-22403 -RRB- denotes )
T10220 22403-22404 -COMMA- denotes ,
T10221 22405-22415 VB denotes indicating
T10222 22416-22422 JJ denotes intact
T10223 22423-22428 NN denotes TGF-β
T10224 22429-22438 NN denotes signaling
T10225 23318-23323 VB denotes Taken
T10226 23324-23332 RB denotes together
T10227 23332-23333 -COMMA- denotes ,
T10228 23334-23339 DT denotes these
T10229 23340-23347 NN denotes results
T10230 23348-23360 VB denotes demonstrated
T10231 23361-23362 DT denotes a
T10232 23363-23373 JJ denotes repressive
T10233 23374-23378 NN denotes role
T10234 23379-23381 IN denotes of
T10235 23382-23394 JJ denotes IL-4-induced
T10236 23395-23400 NN denotes GATA3
T10237 23401-23414 NN denotes transcription
T10238 23415-23421 NN denotes factor
T10239 23422-23424 IN denotes in
T10240 23425-23428 DT denotes the
T10241 23429-23439 NN denotes generation
T10242 23440-23442 IN denotes of
T10243 23443-23448 NN denotes iTreg
T10244 23449-23454 NN denotes cells
T11599 23457-23462 NN denotes GATA3
T11600 23463-23472 VB denotes Represses
T11601 23473-23476 DT denotes the
T11602 23477-23482 NN denotes FOXP3
T11603 23483-23491 NN denotes Promoter
T11604 23492-23494 TO denotes To
T11605 23495-23506 VB denotes investigate
T11606 23507-23510 DT denotes the
T11607 23511-23520 JJ denotes molecular
T11608 23521-23530 NN denotes mechanism
T11609 23531-23533 IN denotes of
T11610 23534-23548 JJ denotes GATA3-mediated
T11611 23549-23559 NN denotes repression
T11612 23560-23562 IN denotes of
T11613 23563-23568 JJ denotes human
T11614 23569-23574 NN denotes FOXP3
T11615 23574-23575 -COMMA- denotes ,
T11616 23576-23579 DT denotes the
T11617 23580-23585 JJ denotes human
T11618 23586-23591 NN denotes FOXP3
T11619 23592-23600 NN denotes promoter
T11620 23601-23604 VB denotes was
T11621 23605-23612 VB denotes studied
T11622 23613-23616 CC denotes and
T11623 23617-23618 DT denotes a
T11624 23619-23630 JJ denotes palindromic
T11625 23631-23638 NN denotes binding
T11626 23639-23643 NN denotes site
T11627 23644-23647 IN denotes for
T11628 23648-23653 NN denotes GATA3
T11629 23654-23657 VB denotes was
T11630 23658-23668 VB denotes discovered
T11631 23670-23673 DT denotes The
T11632 23674-23686 JJ denotes GATA-binding
T11633 23687-23691 NN denotes site
T11634 23692-23694 VB denotes is
T11635 23695-23702 JJ denotes located
T11636 23703-23706 CD denotes 303
T11637 23707-23709 NN denotes bp
T11638 23710-23718 RB denotes upstream
T11639 23719-23723 IN denotes from
T11640 23724-23727 DT denotes the
T11641 23728-23741 NN denotes transcription
T11642 23742-23747 NN denotes start
T11643 23748-23752 NN denotes site
T11644 23753-23754 -LRB- denotes (
T11645 23754-23757 NN denotes TSS
T11646 23757-23758 -RRB- denotes )
T11647 23759-23760 -LRB- denotes [
T11648 23760-23762 CD denotes 24
T11649 23762-23763 -RRB- denotes ]
T11650 23765-23769 DT denotes This
T11651 23770-23774 NN denotes site
T11652 23775-23777 VB denotes is
T11653 23778-23784 RB denotes highly
T11654 23785-23794 VB denotes conserved
T11655 23795-23802 IN denotes between
T11656 23803-23809 NN denotes humans
T11657 23809-23810 -COMMA- denotes ,
T11658 23811-23815 NN denotes mice
T11659 23815-23816 -COMMA- denotes ,
T11660 23817-23820 CC denotes and
T11661 23821-23825 NN denotes rats
T11662 23826-23827 -LRB- denotes (
T11663 23827-23833 NN denotes Figure
T11664 23834-23836 NN denotes S4
T11665 23836-23837 -RRB- denotes )
T11666 23838-23841 CC denotes and
T11667 23842-23845 MD denotes may
T11668 23846-23855 RB denotes therefore
T11669 23856-23860 VB denotes play
T11670 23861-23863 DT denotes an
T11671 23864-23873 JJ denotes important
T11672 23874-23878 NN denotes role
T11673 23879-23881 IN denotes in
T11674 23882-23887 NN denotes FOXP3
T11675 23888-23898 NN denotes regulation
T11676 23900-23903 DT denotes The
T11677 23904-23914 JJ denotes functional
T11678 23915-23924 NN denotes relevance
T11679 23925-23927 IN denotes of
T11680 23928-23932 DT denotes this
T11681 23933-23937 NN denotes site
T11682 23938-23941 VB denotes was
T11683 23942-23949 VB denotes studied
T11684 23950-23955 VB denotes using
T11685 23956-23957 DT denotes a
T11686 23958-23972 NN denotes FOXP3-promoter
T11687 23973-23982 NN denotes construct
T11688 23983-23984 -LRB- denotes [
T11689 23984-23986 CD denotes 24
T11690 23986-23987 -RRB- denotes ]
T11691 23989-23991 PRP denotes We
T11692 23992-24003 VB denotes transfected
T11693 24004-24009 JJ denotes human
T11694 24010-24017 JJ denotes primary
T11695 24018-24022 JJ denotes CD4+
T11696 24023-24024 NN denotes T
T11697 24025-24030 NN denotes cells
T11698 24030-24031 -COMMA- denotes ,
T11699 24032-24034 FW denotes in
T11700 24035-24040 FW denotes vitro
T11701 24041-24055 VB denotes differentiated
T11702 24056-24059 NN denotes Th2
T11703 24060-24065 NN denotes cells
T11704 24065-24066 -COMMA- denotes ,
T11705 24067-24070 CC denotes and
T11706 24071-24077 NN denotes Jurkat
T11707 24078-24083 NN denotes cells
T11708 24084-24085 -LRB- denotes (
T11709 24085-24091 NN denotes Jurkat
T11710 24092-24097 NN denotes cells
T11711 24098-24101 VB denotes are
T11712 24102-24107 VB denotes known
T11713 24108-24110 TO denotes to
T11714 24111-24125 RB denotes constitutively
T11715 24126-24133 VB denotes express
T11716 24134-24139 NN denotes GATA3
T11717 24140-24141 -LRB- denotes [
T11718 24141-24146 CD denotes 25,26
T11719 24146-24147 -RRB- denotes ]
T11720 24147-24148 -RRB- denotes )
T11721 24148-24149 -COMMA- denotes ,
T11722 24150-24153 CC denotes and
T11723 24154-24156 PRP denotes we
T11724 24157-24165 VB denotes measured
T11725 24166-24171 NN denotes FOXP3
T11726 24172-24180 NN denotes promoter
T11727 24181-24189 NN denotes activity
T11728 24191-24194 DT denotes The
T11729 24195-24203 NN denotes promoter
T11730 24204-24207 VB denotes was
T11731 24208-24211 RB denotes not
T11732 24212-24218 JJ denotes active
T11733 24219-24221 IN denotes in
T11734 24222-24225 DT denotes the
T11735 24226-24242 JJ denotes GATA3-expressing
T11736 24243-24247 NN denotes cell
T11737 24248-24252 NN denotes line
T11738 24253-24259 NN denotes Jurkat
T11739 24260-24262 CC denotes or
T11740 24263-24265 IN denotes in
T11741 24266-24269 DT denotes the
T11742 24270-24272 IN denotes in
T11743 24273-24293 JJ denotes vitro-differentiated
T11744 24294-24297 NN denotes Th2
T11745 24298-24303 NN denotes cells
T11746 24303-24304 -COMMA- denotes ,
T11747 24305-24312 IN denotes whereas
T11748 24313-24316 DT denotes the
T11749 24317-24326 NN denotes construct
T11750 24327-24330 VB denotes was
T11751 24331-24337 JJ denotes active
T11752 24338-24340 IN denotes in
T11753 24341-24344 DT denotes the
T11754 24345-24348 NN denotes CD4
T11755 24349-24354 NN denotes cells
T11756 24354-24355 -COMMA- denotes ,
T11757 24356-24361 WDT denotes which
T11758 24362-24369 VB denotes express
T11759 24370-24371 DT denotes a
T11760 24372-24377 JJ denotes lower
T11761 24378-24384 NN denotes amount
T11762 24385-24387 IN denotes of
T11763 24388-24393 NN denotes GATA3
T11764 24394-24395 -LRB- denotes (
T11765 24395-24401 NN denotes Figure
T11766 24402-24404 NN denotes 8A
T11767 24404-24405 -RRB- denotes )
T11768 24407-24421 NN denotes Overexpression
T11769 24422-24424 IN denotes of
T11770 24425-24430 NN denotes GATA3
T11771 24431-24433 IN denotes in
T11772 24434-24439 JJ denotes naive
T11773 24440-24441 NN denotes T
T11774 24442-24447 NN denotes cells
T11775 24448-24458 VB denotes diminished
T11776 24459-24469 NN denotes luciferase
T11777 24470-24478 NN denotes activity
T11778 24479-24481 IN denotes of
T11779 24482-24485 DT denotes the
T11780 24486-24491 NN denotes FOXP3
T11781 24492-24500 NN denotes promoter
T11782 24501-24509 VB denotes compared
T11783 24510-24514 IN denotes with
T11784 24515-24518 DT denotes the
T11785 24519-24526 NN denotes control
T11786 24527-24533 NN denotes vector
T11787 24534-24535 -LRB- denotes (
T11788 24535-24541 NN denotes Figure
T11789 24542-24544 NN denotes 8B
T11790 24544-24545 -RRB- denotes )
T11791 24547-24549 TO denotes To
T11792 24550-24557 RB denotes further
T11793 24558-24565 VB denotes address
T11794 24566-24569 DT denotes the
T11795 24570-24578 NN denotes function
T11796 24579-24581 IN denotes of
T11797 24582-24585 DT denotes the
T11798 24586-24591 NN denotes GATA3
T11799 24592-24596 NN denotes site
T11800 24596-24597 -COMMA- denotes ,
T11801 24598-24600 PRP denotes we
T11802 24601-24609 VB denotes inserted
T11803 24610-24611 DT denotes a
T11804 24612-24625 JJ denotes site-specific
T11805 24626-24634 NN denotes mutation
T11806 24635-24643 VB denotes deleting
T11807 24644-24647 DT denotes the
T11808 24648-24661 JJ denotes GATA3-binding
T11809 24662-24666 NN denotes site
T11810 24668-24672 DT denotes This
T11811 24673-24681 NN denotes mutation
T11812 24682-24691 VB denotes increased
T11813 24692-24702 NN denotes luciferase
T11814 24703-24711 NN denotes activity
T11815 24712-24714 IN denotes by
T11816 24715-24721 RB denotes 3-fold
T11817 24722-24724 IN denotes in
T11818 24725-24731 NN denotes memory
T11819 24732-24743 JJ denotes CD4+CD45RO+
T11820 24744-24745 NN denotes T
T11821 24746-24751 NN denotes cells
T11822 24751-24752 -COMMA- denotes ,
T11823 24753-24760 IN denotes whereas
T11824 24761-24763 DT denotes no
T11825 24764-24774 NN denotes difference
T11826 24775-24778 VB denotes was
T11827 24779-24787 VB denotes observed
T11828 24788-24790 IN denotes in
T11829 24791-24796 JJ denotes naive
T11830 24797-24798 -LRB- denotes (
T11831 24798-24804 NN denotes GATA3–
T11832 24804-24805 -RRB- denotes )
T11833 24806-24817 JJ denotes CD4+CD45RA+
T11834 24818-24819 NN denotes T
T11835 24820-24825 NN denotes cells
T11836 24825-24826 -COMMA- denotes ,
T11837 24827-24836 VB denotes revealing
T11838 24837-24838 DT denotes a
T11839 24839-24848 NN denotes repressor
T11840 24849-24857 NN denotes activity
T11841 24858-24860 IN denotes of
T11842 24861-24866 NN denotes GATA3
T11843 24867-24869 IN denotes on
T11844 24870-24873 DT denotes the
T11845 24874-24879 NN denotes FOXP3
T11846 24880-24888 NN denotes promoter
T11847 24889-24890 -LRB- denotes (
T11848 24890-24896 NN denotes Figure
T11849 24897-24899 NN denotes 8C
T11850 24899-24900 -RRB- denotes )
T11851 24902-24913 RB denotes Furthermore
T11852 24914-24919 NN denotes GATA3
T11853 24920-24925 VB denotes binds
T11854 24926-24934 RB denotes directly
T11855 24935-24937 TO denotes to
T11856 24938-24941 DT denotes the
T11857 24942-24947 NN denotes FOXP3
T11858 24948-24956 NN denotes promoter
T11859 24957-24959 IN denotes as
T11860 24960-24972 VB denotes investigated
T11861 24973-24975 IN denotes by
T11862 24976-24985 JJ denotes pull-down
T11863 24986-24991 NN denotes assay
T11864 24993-24996 NN denotes HEK
T11865 24997-25002 NN denotes cells
T11866 25003-25007 VB denotes were
T11867 25008-25019 RB denotes transiently
T11868 25020-25031 VB denotes transfected
T11869 25032-25036 IN denotes with
T11870 25037-25042 NN denotes GATA3
T11871 25043-25045 CC denotes or
T11872 25046-25047 DT denotes a
T11873 25048-25055 NN denotes control
T11874 25056-25062 NN denotes vector
T11875 25062-25063 -COMMA- denotes ,
T11876 25064-25067 CC denotes and
T11877 25068-25078 VB denotes increasing
T11878 25079-25086 NN denotes amounts
T11879 25087-25089 IN denotes of
T11880 25090-25097 NN denotes lysates
T11881 25098-25102 VB denotes were
T11882 25103-25112 VB denotes incubated
T11883 25113-25117 IN denotes with
T11884 25118-25134 NN denotes oligonucleotides
T11885 25135-25145 VB denotes containing
T11886 25146-25149 DT denotes the
T11887 25150-25155 NN denotes GATA3
T11888 25156-25160 NN denotes site
T11889 25161-25163 IN denotes of
T11890 25164-25167 DT denotes the
T11891 25168-25173 NN denotes FOXP3
T11892 25174-25182 NN denotes promoter
T11893 25183-25185 CC denotes or
T11894 25186-25187 DT denotes a
T11895 25188-25195 NN denotes control
T11896 25196-25211 NN denotes oligonucleotide
T11897 25212-25216 IN denotes with
T11898 25217-25218 DT denotes a
T11899 25219-25226 VB denotes mutated
T11900 25227-25240 JJ denotes GATA3-binding
T11901 25241-25245 NN denotes site
T11902 25247-25252 IN denotes After
T11903 25253-25256 DT denotes the
T11904 25257-25266 NN denotes pull-down
T11905 25266-25267 -COMMA- denotes ,
T11906 25268-25273 NN denotes GATA3
T11907 25274-25281 NN denotes binding
T11908 25282-25285 VB denotes was
T11909 25286-25294 VB denotes detected
T11910 25295-25297 IN denotes by
T11911 25298-25305 JJ denotes Western
T11912 25306-25310 NN denotes blot
T11913 25312-25321 RB denotes Similarly
T11914 25321-25322 -COMMA- denotes ,
T11915 25323-25339 JJ denotes GATA3-expressing
T11916 25340-25343 NN denotes Th2
T11917 25344-25349 NN denotes cells
T11918 25350-25353 CC denotes and
T11919 25354-25359 NN denotes iTreg
T11920 25360-25365 NN denotes cells
T11921 25366-25370 VB denotes were
T11922 25371-25380 VB denotes subjected
T11923 25381-25383 TO denotes to
T11924 25384-25388 DT denotes this
T11925 25389-25397 NN denotes approach
T11926 25399-25403 RB denotes Only
T11927 25404-25407 NN denotes HEK
T11928 25408-25413 NN denotes cells
T11929 25414-25428 VB denotes overexpressing
T11930 25429-25434 NN denotes GATA3
T11931 25435-25438 CC denotes and
T11932 25439-25442 NN denotes Th2
T11933 25443-25448 NN denotes cells
T11934 25449-25455 VB denotes showed
T11935 25456-25469 JJ denotes GATA3-binding
T11936 25470-25478 NN denotes activity
T11937 25479-25480 -LRB- denotes (
T11938 25480-25486 NN denotes Figure
T11939 25487-25489 NN denotes 8D
T11940 25490-25493 CC denotes and
T11941 25494-25496 NN denotes 8E
T11942 25496-25497 -RRB- denotes )
T11943 25499-25504 DT denotes These
T11944 25505-25516 NN denotes experiments
T11945 25517-25529 VB denotes demonstrated
T11946 25530-25534 IN denotes that
T11947 25535-25540 NN denotes GATA3
T11948 25541-25546 VB denotes binds
T11949 25547-25550 DT denotes the
T11950 25551-25562 JJ denotes palindromic
T11951 25563-25568 NN denotes FOXP3
T11952 25569-25577 NN denotes promoter
T11953 25579-25581 TO denotes To
T11954 25582-25586 VB denotes gain
T11955 25587-25595 NN denotes insights
T11956 25596-25600 IN denotes into
T11957 25601-25604 DT denotes the
T11958 25605-25607 FW denotes in
T11959 25608-25612 FW denotes vivo
T11960 25613-25622 NN denotes situation
T11961 25622-25623 -COMMA- denotes ,
T11962 25624-25626 PRP denotes we
T11963 25627-25636 VB denotes performed
T11964 25637-25638 DT denotes a
T11965 25639-25648 NN denotes chromatin
T11966 25649-25668 NN denotes immunoprecipitation
T11967 25669-25670 -LRB- denotes (
T11968 25670-25674 NN denotes ChIP
T11969 25674-25675 -RRB- denotes )
T11970 25676-25681 VB denotes using
T11971 25682-25684 DT denotes an
T11972 25685-25695 JJ denotes anti-GATA3
T11973 25696-25704 NN denotes antibody
T11974 25705-25708 CC denotes and
T11975 25709-25715 VB denotes showed
T11976 25716-25720 IN denotes that
T11977 25721-25726 NN denotes GATA3
T11978 25727-25732 VB denotes binds
T11979 25733-25735 TO denotes to
T11980 25736-25739 DT denotes the
T11981 25740-25745 NN denotes FOXP3
T11982 25746-25754 NN denotes promoter
T11983 25755-25761 NN denotes region
T11984 25762-25764 IN denotes in
T11985 25765-25768 NN denotes Th2
T11986 25769-25774 NN denotes cells
T11987 25774-25775 -COMMA- denotes ,
T11988 25776-25779 CC denotes but
T11989 25780-25783 RB denotes not
T11990 25784-25786 IN denotes in
T11991 25787-25792 NN denotes iTreg
T11992 25793-25798 NN denotes cells
T11993 25799-25800 -LRB- denotes (
T11994 25800-25806 NN denotes Figure
T11995 25807-25809 NN denotes 7F
T11996 25809-25810 -RRB- denotes )
T11997 25812-25817 VB denotes Taken
T11998 25818-25826 RB denotes together
T11999 25827-25832 DT denotes these
T12000 25833-25837 NN denotes data
T12001 25838-25849 VB denotes demonstrate
T12002 25850-25854 IN denotes that
T12003 25855-25858 DT denotes the
T12004 25859-25872 NN denotes GATA3-binding
T12005 25873-25875 TO denotes to
T12006 25876-25879 DT denotes the
T12007 25880-25885 NN denotes FOXP3
T12008 25886-25894 NN denotes promoter
T12009 25895-25897 VB denotes is
T12010 25898-25908 VB denotes repressing
T12011 25909-25914 NN denotes FOXP3
T12012 25915-25925 NN denotes expression
T13704 27625-27628 DT denotes The
T13705 27629-27636 JJ denotes current
T13706 27637-27642 NN denotes study
T13707 27643-27650 VB denotes reveals
T13708 27651-27655 IN denotes that
T13709 27656-27661 NN denotes FOXP3
T13710 27662-27671 NN denotes induction
T13711 27671-27672 -COMMA- denotes ,
T13712 27673-27675 DT denotes an
T13713 27676-27685 JJ denotes important
T13714 27686-27690 NN denotes step
T13715 27691-27693 IN denotes in
T13716 27694-27699 NN denotes iTreg
T13717 27700-27710 NN denotes commitment
T13718 27710-27711 -COMMA- denotes ,
T13719 27712-27714 VB denotes is
T13720 27715-27724 VB denotes inhibited
T13721 27725-27727 IN denotes by
T13722 27728-27733 NN denotes GATA3
T13723 27733-27734 -COMMA- denotes ,
T13724 27735-27740 WDT denotes which
T13725 27741-27743 VB denotes is
T13726 27744-27747 DT denotes the
T13727 27748-27751 JJ denotes key
T13728 27752-27761 NN denotes regulator
T13729 27762-27765 IN denotes for
T13730 27766-27778 NN denotes polarization
T13731 27779-27785 IN denotes toward
T13732 27786-27789 NN denotes Th2
T13733 27790-27795 NN denotes cells
T13734 27797-27802 IN denotes After
T13735 27803-27818 NN denotes differentiation
T13736 27818-27819 -COMMA- denotes ,
T13737 27820-27823 DT denotes the
T13738 27824-27832 NN denotes effector
T13739 27833-27836 NN denotes Th2
T13740 27837-27842 NN denotes cells
T13741 27843-27849 VB denotes become
T13742 27850-27860 JJ denotes refractory
T13743 27861-27863 TO denotes to
T13744 27864-27874 NN denotes conversion
T13745 27875-27879 IN denotes into
T13746 27880-27881 DT denotes a
T13747 27882-27888 JJ denotes FOXP3+
T13748 27889-27898 NN denotes phenotype
T13749 27900-27902 IN denotes In
T13750 27903-27913 NN denotes accordance
T13751 27914-27918 IN denotes with
T13752 27919-27924 JJ denotes other
T13753 27925-27932 NN denotes studies
T13754 27932-27933 -COMMA- denotes ,
T13755 27934-27936 PRP denotes we
T13756 27937-27942 VB denotes found
T13757 27943-27947 IN denotes that
T13758 27948-27957 NN denotes CD4+CD25–
T13759 27958-27963 NN denotes cells
T13760 27964-27968 VB denotes were
T13761 27969-27973 JJ denotes able
T13762 27974-27976 TO denotes to
T13763 27977-27988 VB denotes up-regulate
T13764 27989-27994 NN denotes FOXP3
T13765 27995-27996 -LRB- denotes [
T13766 27996-28001 CD denotes 12,27
T13767 28001-28002 -RRB- denotes ]
T13768 28004-28021 JJ denotes Already-committed
T13769 28022-28027 NN denotes cells
T13770 28028-28032 JJ denotes such
T13771 28033-28035 IN denotes as
T13772 28036-28042 NN denotes memory
T13773 28043-28044 NN denotes T
T13774 28045-28050 NN denotes cells
T13775 28051-28054 CC denotes and
T13776 28055-28058 NN denotes Th1
T13777 28059-28064 NN denotes cells
T13778 28065-28071 VB denotes showed
T13779 28072-28076 RB denotes only
T13780 28077-28085 JJ denotes moderate
T13781 28086-28089 CC denotes and
T13782 28090-28099 JJ denotes transient
T13783 28100-28105 NN denotes FOXP3
T13784 28106-28115 NN denotes induction
T13785 28115-28116 -COMMA- denotes ,
T13786 28117-28122 WDT denotes which
T13787 28123-28125 VB denotes is
T13788 28126-28129 RB denotes not
T13789 28130-28140 JJ denotes sufficient
T13790 28141-28143 TO denotes to
T13791 28144-28150 VB denotes change
T13792 28151-28154 DT denotes the
T13793 28155-28164 NN denotes phenotype
T13794 28165-28171 IN denotes toward
T13795 28172-28173 DT denotes a
T13796 28174-28184 JJ denotes regulatory
T13797 28185-28186 NN denotes T
T13798 28187-28191 NN denotes cell
T13799 28192-28199 NN denotes profile
T13800 28201-28203 IN denotes In
T13801 28204-28212 NN denotes contrast
T13802 28212-28213 -COMMA- denotes ,
T13803 28214-28219 JJ denotes naive
T13804 28220-28221 NN denotes T
T13805 28222-28227 NN denotes cells
T13806 28228-28233 MD denotes could
T13807 28234-28245 RB denotes efficiently
T13808 28246-28257 VB denotes up-regulate
T13809 28258-28263 NN denotes FOXP3
T13810 28264-28268 WRB denotes when
T13811 28269-28276 VB denotes treated
T13812 28277-28281 IN denotes with
T13813 28282-28287 NN denotes TGF-β
T13814 28288-28290 TO denotes to
T13815 28291-28297 VB denotes induce
T13816 28298-28303 NN denotes iTreg
T13817 28304-28309 NN denotes cells
T13818 28310-28311 -LRB- denotes [
T13819 28311-28319 CD denotes 10,28–34
T13820 28319-28320 -RRB- denotes ]
T13821 28320-28321 -COMMA- denotes ,
T13822 28322-28332 VB denotes suggesting
T13823 28333-28337 IN denotes that
T13824 28338-28343 NN denotes FOXP3
T13825 28344-28349 VB denotes plays
T13826 28350-28352 DT denotes an
T13827 28353-28362 JJ denotes important
T13828 28363-28367 NN denotes role
T13829 28368-28370 IN denotes in
T13830 28371-28374 DT denotes the
T13831 28375-28380 JJ denotes early
T13832 28381-28396 NN denotes differentiation
T13833 28397-28404 NN denotes process
T13834 28405-28408 CC denotes and
T13835 28409-28412 MD denotes may
T13836 28413-28416 VB denotes act
T13837 28417-28419 IN denotes in
T13838 28420-28421 DT denotes a
T13839 28422-28425 NN denotes way
T13840 28426-28433 JJ denotes similar
T13841 28434-28436 TO denotes to
T13842 28437-28441 DT denotes that
T13843 28442-28447 VB denotes known
T13844 28448-28451 IN denotes for
T13845 28452-28455 DT denotes the
T13846 28456-28463 NN denotes Th1/Th2
T13847 28464-28472 NN denotes decision
T13848 28473-28480 NN denotes factors
T13849 28481-28486 NN denotes T-bet
T13850 28487-28490 CC denotes and
T13851 28491-28496 NN denotes GATA3
T13852 28498-28502 DT denotes This
T13853 28503-28513 NN denotes commitment
T13854 28514-28516 VB denotes is
T13855 28517-28530 VB denotes characterized
T13856 28531-28533 IN denotes by
T13857 28534-28545 JJ denotes competitive
T13858 28546-28556 NN denotes expression
T13859 28557-28559 IN denotes of
T13860 28560-28565 DT denotes these
T13861 28566-28573 NN denotes factors
T13862 28574-28575 -LRB- denotes [
T13863 28575-28580 CD denotes 35,36
T13864 28580-28581 -RRB- denotes ]
T13865 28581-28582 -COMMA- denotes ,
T13866 28583-28588 WDT denotes which
T13867 28589-28591 PRP denotes we
T13868 28592-28596 RB denotes also
T13869 28597-28605 VB denotes observed
T13870 28606-28608 IN denotes in
T13871 28609-28624 VB denotes differentiating
T13872 28625-28631 JJ denotes FOXP3+
T13873 28632-28637 NN denotes iTreg
T13874 28638-28643 NN denotes cells
T13875 28644-28653 VB denotes including
T13876 28654-28655 DT denotes a
T13877 28656-28661 NN denotes phase
T13878 28662-28664 IN denotes of
T13879 28665-28678 NN denotes co-expression
T13880 28678-28679 -COMMA- denotes ,
T13881 28680-28685 WDT denotes which
T13882 28686-28691 VB denotes turns
T13883 28692-28696 IN denotes into
T13884 28697-28711 JJ denotes nonoverlapping
T13885 28712-28722 NN denotes expression
T13886 28723-28727 IN denotes upon
T13887 28728-28737 VB denotes completed
T13888 28738-28753 NN denotes differentiation
T13889 28755-28757 IN denotes In
T13890 28758-28762 DT denotes this
T13891 28763-28774 JJ denotes competitive
T13892 28775-28782 NN denotes process
T13893 28783-28788 NN denotes TGF-β
T13894 28789-28797 VB denotes appeared
T13895 28798-28800 TO denotes to
T13896 28801-28803 VB denotes be
T13897 28804-28813 JJ denotes mandatory
T13898 28814-28817 IN denotes for
T13899 28818-28821 DT denotes the
T13900 28822-28831 NN denotes induction
T13901 28832-28834 IN denotes of
T13902 28835-28840 NN denotes FOXP3
T13903 28840-28841 -COMMA- denotes ,
T13904 28842-28850 RB denotes possibly
T13905 28851-28853 IN denotes by
T13906 28854-28861 VB denotes keeping
T13907 28862-28865 DT denotes the
T13908 28866-28876 NN denotes expression
T13909 28877-28879 IN denotes of
T13910 28880-28885 NN denotes GATA3
T13911 28886-28889 CC denotes and
T13912 28890-28895 NN denotes T-bet
T13913 28896-28899 JJ denotes low
T13914 28900-28901 -LRB- denotes [
T13915 28901-28906 CD denotes 37,38
T13916 28906-28907 -RRB- denotes ]
T13917 28909-28911 IN denotes In
T13918 28912-28920 NN denotes contrast
T13919 28920-28921 -COMMA- denotes ,
T13920 28922-28937 VB denotes differentiating
T13921 28938-28943 JJ denotes naive
T13922 28944-28945 NN denotes T
T13923 28946-28951 NN denotes cells
T13924 28952-28954 IN denotes in
T13925 28955-28958 DT denotes the
T13926 28959-28966 NN denotes absence
T13927 28967-28969 IN denotes of
T13928 28970-28982 NN denotes polarization
T13929 28983-28990 NN denotes factors
T13930 28991-28992 -LRB- denotes (
T13931 28992-28995 NN denotes Th0
T13932 28995-28996 -RRB- denotes )
T13933 28997-29001 JJ denotes such
T13934 29002-29004 IN denotes as
T13935 29005-29009 NN denotes IL-4
T13936 29009-29010 -COMMA- denotes ,
T13937 29011-29016 NN denotes IL-12
T13938 29016-29017 -COMMA- denotes ,
T13939 29018-29020 CC denotes or
T13940 29021-29026 NN denotes TGF-β
T13941 29027-29033 VB denotes showed
T13942 29034-29038 RB denotes only
T13943 29039-29040 DT denotes a
T13944 29041-29050 JJ denotes transient
T13945 29051-29056 NN denotes FOXP3
T13946 29057-29067 NN denotes expression
T13947 29068-29071 CC denotes and
T13948 29072-29078 VB denotes failed
T13949 29079-29081 TO denotes to
T13950 29082-29090 VB denotes generate
T13951 29091-29092 DT denotes a
T13952 29093-29103 NN denotes population
T13953 29104-29106 IN denotes of
T13954 29107-29123 JJ denotes FOXP3-expressing
T13955 29124-29129 NN denotes cells
T13956 29129-29130 -COMMA- denotes ,
T13957 29131-29134 CC denotes but
T13958 29135-29140 NN denotes GATA3
T13959 29141-29144 CC denotes and
T13960 29145-29150 NN denotes T-bet
T13961 29151-29155 VB denotes were
T13962 29156-29168 VB denotes up-regulated
T13963 29169-29170 -LRB- denotes (
T13964 29170-29181 JJ denotes unpublished
T13965 29182-29186 NN denotes data
T13966 29186-29187 -RRB- denotes )
T13967 29189-29202 RB denotes Interestingly
T13968 29202-29203 -COMMA- denotes ,
T13969 29204-29206 IN denotes as
T13970 29207-29209 PRP denotes we
T13971 29210-29213 CC denotes and
T13972 29214-29220 NN denotes others
T13973 29221-29231 RB denotes previously
T13974 29232-29241 VB denotes described
T13975 29241-29242 -COMMA- denotes ,
T13976 29243-29258 JJ denotes FOXP3-promoting
T13977 29259-29266 NN denotes factors
T13978 29266-29267 -COMMA- denotes ,
T13979 29268-29272 JJ denotes such
T13980 29273-29275 IN denotes as
T13981 29276-29289 NN denotes dexamethasone
T13982 29290-29291 -LRB- denotes [
T13983 29291-29293 CD denotes 39
T13984 29293-29294 -RRB- denotes ]
T13985 29294-29295 -COMMA- denotes ,
T13986 29296-29302 NN denotes CTLA-4
T13987 29303-29304 -LRB- denotes [
T13988 29304-29306 CD denotes 40
T13989 29306-29307 -RRB- denotes ]
T13990 29307-29308 -COMMA- denotes ,
T13991 29309-29312 CC denotes and
T13992 29313-29322 NN denotes estrogens
T13993 29323-29324 -LRB- denotes [
T13994 29324-29326 CD denotes 41
T13995 29326-29327 -RRB- denotes ]
T13996 29327-29328 -COMMA- denotes ,
T13997 29329-29332 VB denotes are
T13998 29333-29337 RB denotes also
T13999 29338-29343 VB denotes known
T14000 29344-29346 IN denotes as
T14001 29347-29357 NN denotes inhibitors
T14002 29358-29360 IN denotes of
T14003 29361-29366 NN denotes GATA3
T14004 29367-29377 NN denotes expression
T14005 29378-29379 -LRB- denotes [
T14006 29379-29384 CD denotes 42–45
T14007 29384-29385 -RRB- denotes ]
T14008 29387-29396 RB denotes Therefore
T14009 29397-29402 NN denotes GATA3
T14010 29403-29406 RB denotes not
T14011 29407-29411 RB denotes only
T14012 29412-29419 VB denotes induces
T14013 29420-29435 NN denotes differentiation
T14014 29436-29440 IN denotes into
T14015 29441-29444 NN denotes Th2
T14016 29445-29450 NN denotes cells
T14017 29451-29454 CC denotes but
T14018 29455-29459 RB denotes also
T14019 29460-29468 VB denotes inhibits
T14020 29469-29474 NN denotes FOXP3
T14021 29475-29485 NN denotes expression
T14022 29486-29489 CC denotes and
T14023 29490-29500 NN denotes commitment
T14024 29501-29505 IN denotes into
T14025 29506-29511 NN denotes iTreg
T14026 29512-29517 NN denotes cells
T14027 29519-29522 DT denotes The
T14028 29523-29526 NN denotes Th2
T14029 29527-29535 NN denotes cytokine
T14030 29536-29540 NN denotes IL-4
T14031 29541-29544 CC denotes but
T14032 29545-29548 RB denotes not
T14033 29549-29554 NN denotes IL-13
T14034 29555-29556 -LRB- denotes (
T14035 29556-29567 JJ denotes unpublished
T14036 29568-29572 NN denotes data
T14037 29572-29573 -RRB- denotes )
T14038 29574-29577 VB denotes was
T14039 29578-29582 JJ denotes able
T14040 29583-29585 TO denotes to
T14041 29586-29593 VB denotes inhibit
T14042 29594-29608 JJ denotes TGF-β–mediated
T14043 29609-29614 NN denotes FOXP3
T14044 29615-29624 NN denotes induction
T14045 29625-29628 CC denotes and
T14046 29629-29638 RB denotes therefore
T14047 29639-29648 VB denotes prevented
T14048 29649-29659 NN denotes conversion
T14049 29660-29664 IN denotes into
T14050 29665-29668 DT denotes the
T14051 29669-29679 JJ denotes regulatory
T14052 29680-29689 NN denotes phenotype
T14053 29691-29693 TO denotes To
T14054 29694-29699 VB denotes prove
T14055 29700-29703 DT denotes the
T14056 29704-29714 JJ denotes inhibitory
T14057 29715-29721 NN denotes effect
T14058 29722-29724 IN denotes of
T14059 29725-29729 NN denotes IL-4
T14060 29730-29732 IN denotes on
T14061 29733-29742 JJ denotes inducible
T14062 29743-29745 CC denotes or
T14063 29746-29753 JJ denotes natural
T14064 29754-29758 NN denotes Treg
T14065 29759-29769 NN denotes commitment
T14066 29770-29772 FW denotes in
T14067 29773-29777 FW denotes vivo
T14068 29777-29778 -COMMA- denotes ,
T14069 29779-29781 PRP denotes we
T14070 29782-29789 VB denotes treated
T14071 29790-29794 NN denotes mice
T14072 29795-29799 IN denotes with
T14073 29800-29804 NN denotes IL-4
T14074 29805-29808 CC denotes and
T14075 29809-29818 NN denotes anti-IL-4
T14076 29820-29824 DT denotes This
T14077 29825-29828 VB denotes has
T14078 29829-29833 VB denotes been
T14079 29834-29839 VB denotes shown
T14080 29840-29842 TO denotes to
T14081 29843-29851 VB denotes increase
T14082 29852-29855 DT denotes the
T14083 29856-29862 NN denotes effect
T14084 29863-29865 IN denotes of
T14085 29866-29869 DT denotes the
T14086 29870-29878 NN denotes cytokine
T14087 29879-29881 FW denotes in
T14088 29882-29886 FW denotes vivo
T14089 29887-29888 -LRB- denotes [
T14090 29888-29890 CD denotes 19
T14091 29890-29891 -RRB- denotes ]
T14092 29893-29897 RB denotes Only
T14093 29898-29901 DT denotes the
T14094 29902-29911 NN denotes IL-4/IL-4
T14095 29912-29915 NN denotes mAb
T14096 29916-29923 NN denotes complex
T14097 29924-29932 VB denotes resulted
T14098 29933-29935 IN denotes in
T14099 29936-29937 DT denotes a
T14100 29938-29946 NN denotes decrease
T14101 29947-29949 IN denotes of
T14102 29950-29953 DT denotes the
T14103 29954-29960 NN denotes amount
T14104 29961-29963 IN denotes of
T14105 29964-29971 JJ denotes natural
T14106 29972-29974 CC denotes or
T14107 29975-29984 JJ denotes inducible
T14108 29985-29989 NN denotes Treg
T14109 29990-29991 -LRB- denotes (
T14110 29991-29996 JJ denotes CD25+
T14111 29997-30000 CC denotes and
T14112 30001-30007 JJ denotes Foxp3+
T14113 30007-30008 -RRB- denotes )
T14114 30009-30014 NN denotes cells
T14115 30015-30016 CD denotes 7
T14116 30017-30018 NN denotes d
T14117 30019-30024 IN denotes after
T14118 30025-30034 NN denotes treatment
T14119 30036-30039 PRP-DOLLAR- denotes Our
T14120 30040-30047 NN denotes results
T14121 30048-30055 VB denotes suggest
T14122 30056-30060 IN denotes that
T14123 30061-30065 NN denotes IL-4
T14124 30066-30068 VB denotes is
T14125 30069-30073 RB denotes only
T14126 30074-30085 VB denotes interfering
T14127 30086-30090 IN denotes with
T14128 30091-30094 DT denotes the
T14129 30095-30110 NN denotes differentiation
T14130 30111-30113 IN denotes of
T14131 30114-30119 JJ denotes naive
T14132 30120-30121 NN denotes T
T14133 30122-30127 NN denotes cells
T14134 30128-30132 IN denotes into
T14135 30133-30138 NN denotes iTreg
T14136 30139-30144 NN denotes cells
T14137 30146-30149 CC denotes But
T14138 30150-30155 IN denotes since
T14139 30156-30157 DT denotes a
T14140 30158-30169 NN denotes distinction
T14141 30170-30172 IN denotes of
T14142 30173-30178 NN denotes nTreg
T14143 30179-30182 CC denotes and
T14144 30183-30188 NN denotes iTreg
T14145 30189-30194 NN denotes cells
T14146 30195-30197 VB denotes is
T14147 30198-30207 RB denotes currently
T14148 30208-30211 RB denotes not
T14149 30212-30220 JJ denotes possible
T14150 30220-30221 -COMMA- denotes ,
T14151 30222-30229 IN denotes because
T14152 30230-30235 NN denotes iTreg
T14153 30236-30241 NN denotes cells
T14154 30242-30246 RB denotes also
T14155 30247-30258 RB denotes transiently
T14156 30259-30266 VB denotes express
T14157 30267-30271 NN denotes CD25
T14158 30272-30277 IN denotes after
T14159 30278-30288 NN denotes activation
T14160 30288-30289 -COMMA- denotes ,
T14161 30290-30292 PRP denotes we
T14162 30293-30296 MD denotes can
T14163 30296-30299 RB denotes not
T14164 30300-30307 VB denotes exclude
T14165 30308-30312 DT denotes that
T14166 30313-30317 NN denotes IL-4
T14167 30318-30321 MD denotes may
T14168 30322-30326 RB denotes also
T14169 30327-30334 VB denotes inhibit
T14170 30335-30340 NN denotes Foxp3
T14171 30341-30351 NN denotes expression
T14172 30352-30354 IN denotes in
T14173 30355-30360 NN denotes nTreg
T14174 30361-30366 NN denotes cells
T14175 30367-30369 FW denotes in
T14176 30370-30374 FW denotes vivo
T14177 30375-30377 CC denotes or
T14178 30378-30382 IN denotes that
T14179 30383-30393 JJ denotes additional
T14180 30394-30401 NN denotes effects
T14181 30402-30405 MD denotes may
T14182 30406-30416 VB denotes contribute
T14183 30417-30419 TO denotes to
T14184 30420-30423 DT denotes the
T14185 30424-30432 VB denotes observed
T14186 30433-30437 NN denotes drop
T14187 30438-30440 IN denotes in
T14188 30441-30446 NN denotes Foxp3
T14189 30447-30457 NN denotes expression
T14190 30459-30463 NN denotes IL-4
T14191 30464-30467 VB denotes has
T14192 30468-30475 RB denotes already
T14193 30476-30480 VB denotes been
T14194 30481-30486 VB denotes shown
T14195 30487-30489 TO denotes to
T14196 30490-30500 RB denotes negatively
T14197 30501-30509 VB denotes regulate
T14198 30510-30513 DT denotes the
T14199 30514-30525 NN denotes development
T14200 30526-30528 IN denotes of
T14201 30529-30534 JJ denotes naive
T14202 30535-30536 NN denotes T
T14203 30537-30542 NN denotes cells
T14204 30543-30547 IN denotes into
T14205 30548-30551 NN denotes Th1
T14206 30552-30554 CC denotes or
T14207 30555-30558 DT denotes the
T14208 30559-30574 JJ denotes IL-17–producing
T14209 30575-30576 NN denotes T
T14210 30577-30582 NN denotes cells
T14211 30583-30584 -LRB- denotes (
T14212 30584-30588 NN denotes Th17
T14213 30588-30589 -RRB- denotes )
T14214 30590-30591 -LRB- denotes [
T14215 30591-30596 CD denotes 46,47
T14216 30596-30597 -RRB- denotes ]
T14217 30599-30606 JJ denotes Similar
T14218 30607-30614 NN denotes effects
T14219 30615-30619 VB denotes have
T14220 30620-30624 VB denotes been
T14221 30625-30633 RB denotes recently
T14222 30634-30643 VB denotes described
T14223 30644-30647 IN denotes for
T14224 30648-30652 NN denotes IL-6
T14225 30652-30653 -COMMA- denotes ,
T14226 30654-30659 WDT denotes which
T14227 30659-30660 -COMMA- denotes ,
T14228 30661-30669 VB denotes combined
T14229 30670-30674 IN denotes with
T14230 30675-30680 NN denotes TGF-β
T14231 30680-30681 -COMMA- denotes ,
T14232 30682-30690 VB denotes inhibits
T14233 30691-30694 DT denotes the
T14234 30695-30705 NN denotes generation
T14235 30706-30708 IN denotes of
T14236 30709-30714 NN denotes iTreg
T14237 30715-30720 NN denotes cells
T14238 30721-30724 CC denotes and
T14239 30725-30732 VB denotes induces
T14240 30733-30748 NN denotes differentiation
T14241 30749-30753 IN denotes into
T14242 30754-30757 DT denotes the
T14243 30758-30762 NN denotes Th17
T14244 30763-30768 NN denotes cells
T14245 30769-30771 IN denotes by
T14246 30772-30774 DT denotes an
T14247 30775-30782 JJ denotes unknown
T14248 30783-30792 NN denotes mechanism
T14249 30793-30794 -LRB- denotes [
T14250 30794-30799 CD denotes 48,49
T14251 30799-30800 -RRB- denotes ]
T14252 30802-30806 RB denotes Thus
T14253 30807-30810 DT denotes the
T14254 30811-30823 NN denotes polarization
T14255 30824-30828 IN denotes into
T14256 30829-30834 NN denotes iTreg
T14257 30835-30840 NN denotes cells
T14258 30841-30843 VB denotes is
T14259 30844-30854 RB denotes negatively
T14260 30855-30864 VB denotes regulated
T14261 30865-30867 IN denotes by
T14262 30868-30871 DT denotes the
T14263 30872-30880 NN denotes effector
T14264 30881-30890 NN denotes cytokines
T14265 30891-30895 NN denotes IL-4
T14266 30896-30899 CC denotes and
T14267 30900-30904 NN denotes IL-6
T14268 30906-30910 NN denotes IL-4
T14269 30911-30914 VB denotes has
T14270 30915-30919 VB denotes been
T14271 30920-30930 RB denotes previously
T14272 30931-30936 VB denotes shown
T14273 30937-30939 TO denotes to
T14274 30940-30946 VB denotes induce
T14275 30947-30950 DT denotes the
T14276 30951-30961 NN denotes generation
T14277 30962-30964 IN denotes of
T14278 30965-30971 JJ denotes FOXP3+
T14279 30972-30976 NN denotes Treg
T14280 30977-30982 NN denotes cells
T14281 30983-30986 IN denotes out
T14282 30987-30989 IN denotes of
T14283 30990-30999 NN denotes CD4+CD25–
T14284 31000-31001 -LRB- denotes [
T14285 31001-31003 CD denotes 50
T14286 31003-31004 -RRB- denotes ]
T14287 31006-31008 IN denotes In
T14288 31009-31014 DT denotes those
T14289 31015-31026 NN denotes experiments
T14290 31026-31027 -COMMA- denotes ,
T14291 31028-31031 DT denotes the
T14292 31032-31046 NN denotes concentrations
T14293 31047-31049 IN denotes of
T14294 31050-31054 NN denotes IL-4
T14295 31055-31059 VB denotes used
T14296 31060-31064 VB denotes were
T14297 31065-31068 JJ denotes low
T14298 31068-31069 -COMMA- denotes ,
T14299 31070-31073 CC denotes and
T14300 31074-31076 IN denotes as
T14301 31077-31079 PRP denotes we
T14302 31080-31084 RB denotes also
T14303 31085-31093 VB denotes observed
T14304 31093-31094 -COMMA- denotes ,
T14305 31095-31099 NN denotes IL-4
T14306 31100-31102 IN denotes at
T14307 31103-31106 JJ denotes low
T14308 31107-31120 NN denotes concentration
T14309 31121-31129 RB denotes slightly
T14310 31130-31138 VB denotes enhanced
T14311 31139-31144 NN denotes FOXP3
T14312 31145-31155 NN denotes expression
T14313 31157-31168 RB denotes Importantly
T14314 31168-31169 -COMMA- denotes ,
T14315 31170-31175 DT denotes these
T14316 31176-31190 NN denotes concentrations
T14317 31191-31195 VB denotes were
T14318 31196-31199 RB denotes not
T14319 31200-31210 JJ denotes sufficient
T14320 31211-31213 TO denotes to
T14321 31214-31220 VB denotes induce
T14322 31221-31226 NN denotes GATA3
T14323 31227-31237 NN denotes expression
T14324 31239-31243 NN denotes IL-4
T14325 31244-31247 MD denotes may
T14326 31248-31253 VB denotes favor
T14327 31254-31267 NN denotes proliferation
T14328 31268-31270 IN denotes of
T14329 31271-31276 NN denotes nTreg
T14330 31277-31282 NN denotes cells
T14331 31283-31284 -LRB- denotes [
T14332 31284-31286 CD denotes 47
T14333 31286-31287 -RRB- denotes ]
T14334 31288-31290 CC denotes or
T14335 31291-31299 RB denotes directly
T14336 31300-31308 VB denotes regulate
T14337 31309-31314 NN denotes FOXP3
T14338 31315-31325 NN denotes expression
T14339 31326-31328 IN denotes in
T14340 31329-31330 DT denotes a
T14341 31331-31345 JJ denotes STAT-dependent
T14342 31346-31353 NN denotes fashion
T14343 31354-31355 -LRB- denotes [
T14344 31355-31357 CD denotes 51
T14345 31357-31358 -RRB- denotes ]
T14346 31360-31365 IN denotes Since
T14347 31366-31371 NN denotes IL-13
T14348 31372-31376 VB denotes does
T14349 31377-31380 RB denotes not
T14350 31381-31392 RB denotes effectively
T14351 31393-31399 VB denotes reduce
T14352 31400-31405 NN denotes FOXP3
T14353 31406-31409 CC denotes and
T14354 31410-31415 VB denotes fails
T14355 31416-31418 TO denotes to
T14356 31419-31425 VB denotes induce
T14357 31426-31431 NN denotes GATA3
T14358 31431-31432 -COMMA- denotes ,
T14359 31433-31435 PRP denotes we
T14360 31436-31448 VB denotes hypothesized
T14361 31449-31453 IN denotes that
T14362 31454-31457 DT denotes the
T14363 31458-31472 JJ denotes IL-4–dependent
T14364 31473-31483 NN denotes inhibition
T14365 31484-31486 IN denotes of
T14366 31487-31492 NN denotes FOXP3
T14367 31493-31498 MD denotes could
T14368 31499-31501 VB denotes be
T14369 31502-31510 VB denotes mediated
T14370 31511-31513 IN denotes by
T14371 31514-31519 NN denotes GATA3
T14372 31521-31523 IN denotes In
T14373 31524-31528 NN denotes fact
T14374 31528-31529 -COMMA- denotes ,
T14375 31530-31544 JJ denotes GATA3-inducing
T14376 31545-31549 NN denotes IL-4
T14377 31550-31564 NN denotes concentrations
T14378 31565-31574 VB denotes repressed
T14379 31575-31589 JJ denotes TGF-β–mediated
T14380 31590-31595 NN denotes FOXP3
T14381 31596-31606 NN denotes expression
T14382 31606-31607 -COMMA- denotes ,
T14383 31608-31615 IN denotes whereas
T14384 31616-31620 NN denotes IL-4
T14385 31621-31623 RB denotes as
T14386 31624-31628 RB denotes well
T14387 31629-31631 IN denotes as
T14388 31632-31637 NN denotes TGF-β
T14389 31638-31647 NN denotes signaling
T14390 31648-31652 VB denotes were
T14391 31653-31659 JJ denotes intact
T14392 31661-31665 DT denotes This
T14393 31666-31672 NN denotes result
T14394 31673-31682 VB denotes suggested
T14395 31683-31684 DT denotes a
T14396 31685-31696 JJ denotes competitive
T14397 31697-31706 NN denotes mechanism
T14398 31707-31714 IN denotes between
T14399 31715-31720 NN denotes GATA3
T14400 31721-31724 CC denotes and
T14401 31725-31730 NN denotes FOXP3
T14402 31731-31744 NN denotes transcription
T14403 31745-31752 NN denotes factors
T14404 31753-31755 IN denotes in
T14405 31756-31767 VB denotes determining
T14406 31768-31775 NN denotes lineage
T14407 31776-31786 NN denotes commitment
T14408 31787-31793 IN denotes during
T14409 31794-31797 DT denotes the
T14410 31798-31803 JJ denotes early
T14411 31804-31809 NN denotes phase
T14412 31810-31812 IN denotes of
T14413 31813-31828 NN denotes differentiation
T14414 31830-31841 RB denotes Accordingly
T14415 31841-31842 -COMMA- denotes ,
T14416 31843-31845 PRP denotes we
T14417 31846-31858 VB denotes investigated
T14418 31859-31868 RB denotes naturally
T14419 31869-31873 JJ denotes high
T14420 31874-31890 NN denotes GATA3-expressing
T14421 31891-31896 NN denotes cells
T14422 31897-31900 CC denotes and
T14423 31901-31910 VB denotes confirmed
T14424 31911-31914 DT denotes the
T14425 31915-31922 NN denotes absence
T14426 31923-31925 IN denotes of
T14427 31926-31931 NN denotes FOXP3
T14428 31933-31940 NN denotes Protein
T14429 31941-31953 NN denotes transduction
T14430 31954-31956 IN denotes of
T14431 31957-31962 NN denotes GATA3
T14432 31963-31967 IN denotes into
T14433 31968-31973 JJ denotes naive
T14434 31974-31975 NN denotes T
T14435 31976-31981 NN denotes cells
T14436 31982-31991 VB denotes inhibited
T14437 31992-31997 NN denotes FOXP3
T14438 31998-32007 NN denotes induction
T14439 32008-32010 IN denotes in
T14440 32011-32016 JJ denotes human
T14441 32016-32017 -COMMA- denotes ,
T14442 32018-32033 VB denotes differentiating
T14443 32033-32034 -COMMA- denotes ,
T14444 32035-32040 JJ denotes naive
T14445 32041-32042 NN denotes T
T14446 32043-32048 NN denotes cells
T14447 32050-32054 DT denotes This
T14448 32055-32065 JJ denotes inhibitory
T14449 32066-32072 NN denotes effect
T14450 32073-32075 IN denotes of
T14451 32076-32081 NN denotes GATA3
T14452 32082-32085 VB denotes was
T14453 32086-32093 RB denotes further
T14454 32094-32103 VB denotes confirmed
T14455 32104-32106 IN denotes in
T14456 32107-32113 NN denotes BALB/c
T14457 32114-32124 JJ denotes transgenic
T14458 32125-32129 NN denotes mice
T14459 32129-32130 -COMMA- denotes ,
T14460 32131-32141 VB denotes expressing
T14461 32142-32147 NN denotes GATA3
T14462 32148-32150 IN denotes in
T14463 32151-32152 NN denotes T
T14464 32153-32158 NN denotes cells
T14465 32159-32160 -LRB- denotes (
T14466 32160-32167 NN denotes DO11.10
T14467 32167-32168 -COLON- denotes :
T14468 32168-32177 JJ denotes CD2-GATA3
T14469 32178-32188 JJ denotes transgenic
T14470 32189-32193 NN denotes mice
T14471 32193-32194 -RRB- denotes )
T14472 32196-32198 IN denotes In
T14473 32199-32203 NN denotes line
T14474 32204-32208 IN denotes with
T14475 32209-32212 DT denotes the
T14476 32213-32222 JJ denotes transient
T14477 32223-32237 NN denotes overexpression
T14478 32238-32240 IN denotes of
T14479 32241-32246 NN denotes GATA3
T14480 32247-32249 IN denotes in
T14481 32250-32255 JJ denotes human
T14482 32256-32257 NN denotes T
T14483 32258-32263 NN denotes cells
T14484 32263-32264 -COMMA- denotes ,
T14485 32265-32270 NN denotes cells
T14486 32271-32273 IN denotes of
T14487 32274-32279 DT denotes these
T14488 32280-32284 NN denotes mice
T14489 32285-32291 VB denotes failed
T14490 32292-32294 TO denotes to
T14491 32295-32301 VB denotes induce
T14492 32302-32307 NN denotes FOXP3
T14493 32308-32318 NN denotes expression
T14494 32319-32323 IN denotes upon
T14495 32324-32332 NN denotes exposure
T14496 32333-32337 IN denotes with
T14497 32338-32345 NN denotes antigen
T14498 32346-32348 IN denotes in
T14499 32349-32352 DT denotes the
T14500 32353-32361 NN denotes presence
T14501 32362-32364 IN denotes of
T14502 32365-32370 NN denotes TGF-β
T14503 32372-32382 RB denotes Strikingly
T14504 32382-32383 -COMMA- denotes ,
T14505 32384-32387 DT denotes the
T14506 32388-32395 NN denotes DO11.10
T14507 32395-32396 -COLON- denotes :
T14508 32396-32405 NN denotes CD2-GATA3
T14509 32406-32410 NN denotes mice
T14510 32411-32413 VB denotes do
T14511 32414-32418 VB denotes have
T14512 32419-32429 JJ denotes peripheral
T14513 32430-32436 JJ denotes FOXP3+
T14514 32437-32442 NN denotes cells
T14515 32442-32443 -COMMA- denotes ,
T14516 32444-32449 WDT denotes which
T14517 32450-32457 RB denotes however
T14518 32458-32467 VB denotes displayed
T14519 32468-32469 DT denotes a
T14520 32470-32472 CD denotes 10
T14521 32472-32473 NN denotes %
T14522 32473-32476 CD denotes –25
T14523 32476-32477 NN denotes %
T14524 32478-32483 JJ denotes lower
T14525 32484-32493 NN denotes frequency
T14526 32494-32502 VB denotes compared
T14527 32503-32505 TO denotes to
T14528 32506-32515 JJ denotes wild-type
T14529 32516-32523 NN denotes DO11.10
T14530 32524-32528 NN denotes mice
T14531 32530-32534 RB denotes Thus
T14532 32535-32540 NN denotes GATA3
T14533 32541-32550 VB denotes restrains
T14534 32551-32554 DT denotes the
T14535 32555-32566 NN denotes development
T14536 32567-32569 IN denotes of
T14537 32570-32577 JJ denotes certain
T14538 32578-32582 NN denotes Treg
T14539 32583-32590 NN denotes subsets
T14540 32590-32591 -COMMA- denotes ,
T14541 32592-32602 RB denotes presumably
T14542 32603-32606 DT denotes the
T14543 32607-32616 JJ denotes inducible
T14544 32616-32617 -COMMA- denotes ,
T14545 32618-32628 JJ denotes peripheral
T14546 32629-32639 NN denotes population
T14547 32640-32643 CC denotes and
T14548 32644-32647 RB denotes not
T14549 32648-32653 DT denotes those
T14550 32654-32656 IN denotes of
T14551 32657-32663 JJ denotes thymic
T14552 32664-32670 NN denotes origin
T14553 32672-32678 JJ denotes Thymic
T14554 32679-32680 NN denotes T
T14555 32681-32686 NN denotes cells
T14556 32687-32694 VB denotes undergo
T14557 32695-32696 DT denotes a
T14558 32697-32706 JJ denotes different
T14559 32707-32717 NN denotes maturation
T14560 32718-32725 NN denotes process
T14561 32725-32726 -COMMA- denotes ,
T14562 32727-32732 WDT denotes which
T14563 32733-32736 MD denotes may
T14564 32737-32744 VB denotes explain
T14565 32745-32748 DT denotes the
T14566 32749-32762 NN denotes insensitivity
T14567 32763-32765 IN denotes of
T14568 32766-32771 NN denotes nTreg
T14569 32772-32774 TO denotes to
T14570 32775-32780 NN denotes GATA3
T14571 32781-32795 NN denotes overexpression
T14572 32796-32797 -LRB- denotes [
T14573 32797-32799 CD denotes 52
T14574 32799-32800 -RRB- denotes ]
T14575 32802-32804 IN denotes In
T14576 32805-32813 NN denotes contrast
T14577 32814-32816 TO denotes to
T14578 32817-32820 DT denotes the
T14579 32821-32840 JJ denotes Th2-differentiating
T14580 32841-32844 CC denotes and
T14581 32845-32861 JJ denotes iTreg-inhibiting
T14582 32862-32870 NN denotes function
T14583 32871-32873 IN denotes of
T14584 32874-32879 NN denotes GATA3
T14585 32880-32882 IN denotes in
T14586 32883-32893 JJ denotes peripheral
T14587 32894-32895 NN denotes T
T14588 32896-32901 NN denotes cells
T14589 32901-32902 -COMMA- denotes ,
T14590 32903-32908 NN denotes GATA3
T14591 32909-32913 VB denotes acts
T14592 32914-32916 IN denotes in
T14593 32917-32920 DT denotes the
T14594 32921-32927 NN denotes thymus
T14595 32928-32936 RB denotes together
T14596 32937-32941 IN denotes with
T14597 32942-32947 JJ denotes other
T14598 32948-32961 NN denotes transcription
T14599 32962-32969 NN denotes factors
T14600 32970-32974 JJ denotes such
T14601 32975-32977 IN denotes as
T14602 32978-32981 DT denotes the
T14603 32982-32991 NNP denotes Repressor
T14604 32992-32994 IN denotes of
T14605 32995-33000 NN denotes GATA3
T14606 33001-33002 -LRB- denotes (
T14607 33002-33005 NN denotes ROG
T14608 33005-33006 -RRB- denotes )
T14609 33007-33009 IN denotes in
T14610 33010-33013 DT denotes the
T14611 33014-33029 NN denotes differentiation
T14612 33030-33037 NN denotes process
T14613 33038-33044 IN denotes toward
T14614 33045-33048 NN denotes CD8
T14615 33049-33054 NN denotes cells
T14616 33055-33056 -LRB- denotes [
T14617 33056-33061 CD denotes 53,54
T14618 33061-33062 -RRB- denotes ]
T14619 33063-33065 CC denotes or
T14620 33066-33078 VB denotes participates
T14621 33079-33081 IN denotes in
T14622 33082-33089 JJ denotes complex
T14623 33090-33105 JJ denotes transcriptional
T14624 33106-33114 NN denotes feedback
T14625 33115-33122 NN denotes network
T14626 33123-33125 TO denotes to
T14627 33126-33134 VB denotes regulate
T14628 33135-33150 JJ denotes sympathoadrenal
T14629 33151-33166 NN denotes differentiation
T14630 33167-33168 -LRB- denotes [
T14631 33168-33170 CD denotes 55
T14632 33170-33171 -RRB- denotes ]
T14633 33173-33182 RB denotes Therefore
T14634 33182-33183 -COMMA- denotes ,
T14635 33184-33187 DT denotes the
T14636 33188-33192 NN denotes role
T14637 33193-33195 IN denotes of
T14638 33196-33201 NN denotes GATA3
T14639 33202-33209 VB denotes appears
T14640 33210-33212 TO denotes to
T14641 33213-33215 VB denotes be
T14642 33216-33222 NN denotes tissue
T14643 33223-33231 JJ denotes specific
T14644 33232-33235 CC denotes and
T14645 33236-33239 MD denotes can
T14646 33239-33242 RB denotes not
T14647 33243-33245 VB denotes be
T14648 33246-33257 VB denotes generalized
T14649 33259-33262 PRP-DOLLAR- denotes Our
T14650 33263-33268 NN denotes study
T14651 33269-33281 VB denotes demonstrates
T14652 33282-33286 IN denotes that
T14653 33287-33292 NN denotes GATA3
T14654 33293-33302 VB denotes repressed
T14655 33303-33308 NN denotes FOXP3
T14656 33309-33319 NN denotes expression
T14657 33320-33328 RB denotes directly
T14658 33329-33331 IN denotes by
T14659 33332-33339 VB denotes binding
T14660 33340-33342 TO denotes to
T14661 33343-33346 DT denotes the
T14662 33347-33352 NN denotes FOXP3
T14663 33353-33361 NN denotes promoter
T14664 33362-33368 NN denotes region
T14665 33370-33371 DT denotes A
T14666 33372-33383 JJ denotes palindromic
T14667 33384-33393 NN denotes GATA-site
T14668 33394-33396 VB denotes is
T14669 33397-33404 JJ denotes located
T14670 33405-33408 CD denotes 303
T14671 33409-33411 NN denotes bp
T14672 33412-33420 RB denotes upstream
T14673 33421-33423 IN denotes of
T14674 33424-33427 DT denotes the
T14675 33428-33431 NN denotes TSS
T14676 33432-33434 IN denotes in
T14677 33435-33436 DT denotes a
T14678 33437-33443 RB denotes highly
T14679 33444-33453 VB denotes conserved
T14680 33454-33460 NN denotes region
T14681 33460-33461 -COMMA- denotes ,
T14682 33462-33467 WDT denotes which
T14683 33468-33470 PRP denotes we
T14684 33471-33475 VB denotes have
T14685 33476-33486 RB denotes previously
T14686 33487-33497 VB denotes identified
T14687 33498-33500 IN denotes as
T14688 33501-33504 DT denotes the
T14689 33505-33510 NN denotes FOXP3
T14690 33511-33519 NN denotes promoter
T14691 33520-33521 -LRB- denotes [
T14692 33521-33523 CD denotes 24
T14693 33523-33524 -RRB- denotes ]
T14694 33526-33539 JJ denotes Site-specific
T14695 33540-33548 NN denotes mutation
T14696 33549-33551 IN denotes of
T14697 33552-33556 DT denotes this
T14698 33557-33561 NN denotes site
T14699 33562-33571 VB denotes increased
T14700 33572-33575 DT denotes the
T14701 33576-33584 NN denotes activity
T14702 33585-33587 IN denotes of
T14703 33588-33591 DT denotes the
T14704 33592-33600 NN denotes promoter
T14705 33601-33611 NN denotes constructs
T14706 33611-33612 -COMMA- denotes ,
T14707 33613-33617 RB denotes thus
T14708 33618-33627 VB denotes revealing
T14709 33628-33631 DT denotes the
T14710 33632-33642 JJ denotes repressive
T14711 33643-33649 NN denotes nature
T14712 33650-33652 IN denotes of
T14713 33653-33657 DT denotes this
T14714 33658-33662 NN denotes GATA
T14715 33663-33670 NN denotes element
T14716 33671-33673 IN denotes in
T14717 33674-33680 NN denotes memory
T14718 33681-33682 NN denotes T
T14719 33683-33688 NN denotes cells
T14720 33688-33689 -COMMA- denotes ,
T14721 33690-33695 WDT denotes which
T14722 33696-33705 RB denotes naturally
T14723 33706-33713 VB denotes express
T14724 33714-33719 NN denotes GATA3
T14725 33719-33720 -COMMA- denotes ,
T14726 33721-33728 IN denotes whereas
T14727 33729-33731 DT denotes no
T14728 33732-33742 NN denotes difference
T14729 33743-33746 VB denotes was
T14730 33747-33751 VB denotes seen
T14731 33752-33754 IN denotes in
T14732 33755-33760 JJ denotes naive
T14733 33761-33762 NN denotes T
T14734 33763-33768 NN denotes cells
T14735 33768-33769 -COMMA- denotes ,
T14736 33770-33775 WDT denotes which
T14737 33776-33778 VB denotes do
T14738 33779-33782 RB denotes not
T14739 33783-33790 VB denotes express
T14740 33791-33796 NN denotes GATA3
T14741 33798-33802 DT denotes This
T14742 33803-33814 JJ denotes palindromic
T14743 33815-33819 NN denotes GATA
T14744 33820-33827 NN denotes element
T14745 33828-33830 VB denotes is
T14746 33831-33836 VB denotes bound
T14747 33837-33839 IN denotes by
T14748 33840-33845 NN denotes GATA3
T14749 33846-33853 NN denotes protein
T14750 33854-33856 IN denotes as
T14751 33857-33863 VB denotes proven
T14752 33864-33868 IN denotes with
T14753 33869-33878 JJ denotes pull-down
T14754 33879-33890 NN denotes experiments
T14755 33892-33903 RB denotes Furthermore
T14756 33903-33904 -COMMA- denotes ,
T14757 33905-33907 PRP denotes it
T14758 33908-33910 VB denotes is
T14759 33911-33916 VB denotes shown
T14760 33917-33919 IN denotes by
T14761 33920-33924 NN denotes ChIP
T14762 33925-33929 IN denotes that
T14763 33930-33935 NN denotes GATA3
T14764 33936-33941 VB denotes binds
T14765 33942-33946 DT denotes this
T14766 33947-33954 NN denotes element
T14767 33955-33959 RB denotes also
T14768 33960-33962 IN denotes in
T14769 33963-33969 JJ denotes intact
T14770 33970-33975 NN denotes cells
T14771 33977-33979 PRP denotes It
T14772 33980-33982 VB denotes is
T14773 33983-33988 VB denotes known
T14774 33989-33993 IN denotes that
T14775 33994-33999 NN denotes GATA3
T14776 34000-34003 MD denotes can
T14777 34004-34010 VB denotes induce
T14778 34011-34024 NN denotes transcription
T14779 34025-34027 IN denotes by
T14780 34028-34037 NN denotes chromatin
T14781 34038-34048 VB denotes remodeling
T14782 34049-34050 -LRB- denotes [
T14783 34050-34052 CD denotes 56
T14784 34052-34053 -RRB- denotes ]
T14785 34053-34054 -COMMA- denotes ,
T14786 34055-34057 IN denotes by
T14787 34058-34066 RB denotes directly
T14788 34067-34082 VB denotes transactivating
T14789 34083-34092 NN denotes promoters
T14790 34093-34094 -LRB- denotes [
T14791 34094-34096 CD denotes 36
T14792 34096-34097 -RRB- denotes ]
T14793 34097-34098 -COMMA- denotes ,
T14794 34099-34101 CC denotes or
T14795 34101-34102 -COMMA- denotes ,
T14796 34103-34105 IN denotes as
T14797 34106-34111 VB denotes shown
T14798 34112-34114 IN denotes in
T14799 34115-34118 DT denotes the
T14800 34119-34126 JJ denotes current
T14801 34127-34132 NN denotes study
T14802 34132-34133 -COMMA- denotes ,
T14803 34134-34138 VB denotes acts
T14804 34139-34141 IN denotes as
T14805 34142-34143 DT denotes a
T14806 34144-34153 NN denotes repressor
T14807 34154-34156 IN denotes of
T14808 34157-34161 NN denotes gene
T14809 34162-34172 NN denotes expression
T14810 34173-34174 -LRB- denotes [
T14811 34174-34182 CD denotes 21,57–59
T14812 34182-34183 -RRB- denotes ]
T14813 34185-34194 RB denotes Therefore
T14814 34195-34202 VB denotes keeping
T14815 34203-34208 NN denotes GATA3
T14816 34209-34219 NN denotes expression
T14817 34220-34223 JJ denotes low
T14818 34224-34229 MD denotes might
T14819 34230-34232 VB denotes be
T14820 34233-34241 VB denotes required
T14821 34242-34244 TO denotes to
T14822 34245-34251 VB denotes induce
T14823 34252-34261 JJ denotes efficient
T14824 34262-34268 JJ denotes FOXP3+
T14825 34269-34274 NN denotes iTreg
T14826 34275-34279 NN denotes cell
T14827 34280-34290 NN denotes generation
T14828 34292-34295 DT denotes The
T14829 34296-34305 JJ denotes molecular
T14830 34306-34318 NN denotes interactions
T14831 34319-34327 VB denotes enabling
T14832 34328-34333 NN denotes GATA3
T14833 34334-34336 TO denotes to
T14834 34337-34344 VB denotes inhibit
T14835 34345-34350 NN denotes FOXP3
T14836 34351-34354 VB denotes are
T14837 34355-34358 RB denotes not
T14838 34359-34369 VB denotes identified
T14839 34370-34373 RB denotes yet
T14840 34373-34374 -COMMA- denotes ,
T14841 34375-34378 CC denotes but
T14842 34379-34382 DT denotes the
T14843 34383-34395 JJ denotes GATA-binding
T14844 34396-34400 NN denotes site
T14845 34401-34403 VB denotes is
T14846 34404-34411 VB denotes located
T14847 34412-34420 JJ denotes adjacent
T14848 34421-34423 TO denotes to
T14849 34424-34432 JJ denotes positive
T14850 34432-34433 -COMMA- denotes ,
T14851 34434-34442 VB denotes inducing
T14852 34443-34448 NN denotes sites
T14853 34448-34449 -COMMA- denotes ,
T14854 34450-34458 VB denotes composed
T14855 34459-34461 IN denotes of
T14856 34462-34473 NN denotes AP-1-NFATc2
T14857 34474-34479 NN denotes sites
T14858 34480-34481 -LRB- denotes [
T14859 34481-34483 CD denotes 24
T14860 34483-34484 -RRB- denotes ]
T14861 34484-34485 -COMMA- denotes ,
T14862 34486-34489 CC denotes and
T14863 34490-34495 NN denotes GATA3
T14864 34496-34499 MD denotes may
T14865 34500-34507 VB denotes compete
T14866 34508-34512 IN denotes with
T14867 34513-34516 DT denotes the
T14868 34517-34524 NN denotes binding
T14869 34525-34527 IN denotes of
T14870 34528-34537 NN denotes AP-1/NFAT
T14871 34538-34540 TO denotes to
T14872 34541-34544 DT denotes the
T14873 34545-34553 NN denotes promoter
T14874 34554-34555 -LRB- denotes (
T14875 34555-34566 JJ denotes unpublished
T14876 34567-34579 NN denotes observations
T14877 34579-34580 -RRB- denotes )
T14878 34582-34584 IN denotes In
T14879 34585-34592 NN denotes summary
T14880 34592-34593 -COMMA- denotes ,
T14881 34594-34596 PRP denotes we
T14882 34597-34609 VB denotes demonstrated
T14883 34610-34614 IN denotes that
T14884 34615-34620 NN denotes FOXP3
T14885 34621-34623 VB denotes is
T14886 34624-34634 RB denotes negatively
T14887 34635-34644 VB denotes regulated
T14888 34645-34647 IN denotes by
T14889 34648-34657 NN denotes cytokines
T14890 34658-34662 JJ denotes such
T14891 34663-34665 IN denotes as
T14892 34666-34670 NN denotes IL-4
T14893 34672-34677 NN denotes GATA3
T14894 34678-34682 VB denotes acts
T14895 34683-34685 IN denotes as
T14896 34686-34688 DT denotes an
T14897 34689-34698 NN denotes inhibitor
T14898 34699-34701 IN denotes of
T14899 34702-34707 NN denotes FOXP3
T14900 34708-34718 NN denotes expression
T14901 34719-34721 IN denotes in
T14902 34722-34727 JJ denotes early
T14903 34728-34729 NN denotes T
T14904 34730-34734 NN denotes cell
T14905 34735-34750 NN denotes differentiation
T14906 34750-34751 -COMMA- denotes ,
T14907 34752-34754 RB denotes as
T14908 34755-34759 RB denotes well
T14909 34760-34762 IN denotes as
T14910 34763-34765 IN denotes in
T14911 34766-34780 VB denotes differentiated
T14912 34781-34784 NN denotes Th2
T14913 34785-34790 NN denotes cells
T14914 34791-34793 IN denotes by
T14915 34794-34802 RB denotes directly
T14916 34803-34810 VB denotes binding
T14917 34811-34814 CC denotes and
T14918 34815-34825 VB denotes repressing
T14919 34826-34829 DT denotes the
T14920 34830-34835 NN denotes FOXP3
T14921 34836-34844 NN denotes promoter
T14922 34846-34848 PRP denotes We
T14923 34849-34858 RB denotes therefore
T14924 34859-34867 VB denotes describe
T14925 34868-34869 DT denotes a
T14926 34870-34873 JJ denotes new
T14927 34874-34883 NN denotes mechanism
T14928 34884-34886 IN denotes of
T14929 34887-34890 WRB denotes how
T14930 34891-34895 NN denotes Il-4
T14931 34896-34902 NN denotes avoids
T14932 34903-34912 NN denotes tolerance
T14933 34913-34922 NN denotes induction
T14934 34923-34925 IN denotes by
T14935 34926-34936 VB denotes repressing
T14936 34937-34942 NN denotes FOXP3
T14937 34943-34953 NN denotes expression
T14938 34955-34960 DT denotes These
T14939 34961-34969 NN denotes findings
T14940 34970-34974 MD denotes will
T14941 34975-34979 VB denotes give
T14942 34980-34983 JJ denotes new
T14943 34984-34996 NN denotes perspectives
T14944 34997-35003 IN denotes toward
T14945 35004-35017 VB denotes understanding
T14946 35018-35027 JJ denotes molecular
T14947 35028-35038 NN denotes mechanisms
T14948 35039-35041 IN denotes of
T14949 35042-35047 NN denotes iTreg
T14950 35048-35057 NN denotes induction
T14951 35058-35061 CC denotes and
T14952 35062-35066 RB denotes thus
T14953 35067-35075 NN denotes pathways
T14954 35076-35078 IN denotes of
T14955 35079-35089 JJ denotes peripheral
T14956 35090-35099 NN denotes tolerance
T14957 35100-35109 NN denotes induction
T14958 35109-35110 -COMMA- denotes ,
T14959 35111-35123 RB denotes particularly
T14960 35124-35126 IN denotes in
T14961 35127-35134 NN denotes allergy
T14962 35135-35138 CC denotes and
T14963 35139-35145 NN denotes asthma
T17778 35171-35175 NN denotes Mice
T17779 35177-35183 JJ denotes Normal
T17780 35184-35186 NN denotes B6
T17781 35187-35191 NN denotes mice
T17782 35192-35196 VB denotes were
T17783 35197-35206 VB denotes purchased
T17784 35207-35211 IN denotes from
T17785 35212-35215 DT denotes the
T17786 35216-35223 NNP denotes Jackson
T17787 35224-35236 NNP denotes Laboratories
T17788 35237-35238 -LRB- denotes (
T17789 35238-35241 NNP denotes Bar
T17790 35242-35248 NNP denotes Harbor
T17791 35248-35249 -COMMA- denotes ,
T17792 35250-35255 NNP denotes Maine
T17793 35255-35256 -RRB- denotes )
T17794 35258-35268 JJ denotes Transgenic
T17795 35269-35276 NN denotes DO11.10
T17796 35277-35281 NN denotes mice
T17797 35281-35282 -COMMA- denotes ,
T17798 35283-35293 VB denotes expressing
T17799 35294-35295 DT denotes a
T17800 35296-35297 NN denotes T
T17801 35298-35302 NN denotes cell
T17802 35303-35311 NN denotes receptor
T17803 35312-35315 IN denotes for
T17804 35316-35326 NN denotes OVA323–339
T17805 35327-35334 NN denotes peptide
T17806 35335-35337 IN denotes in
T17807 35338-35341 DT denotes the
T17808 35342-35349 NN denotes context
T17809 35350-35352 IN denotes of
T17810 35353-35357 NN denotes H-2d
T17811 35357-35358 -COMMA- denotes ,
T17812 35359-35363 VB denotes were
T17813 35364-35375 VB denotes backcrossed
T17814 35376-35380 IN denotes with
T17815 35381-35385 NN denotes mice
T17816 35386-35396 VB denotes expressing
T17817 35397-35403 NN denotes GATA-3
T17818 35403-35404 -COMMA- denotes ,
T17819 35405-35411 VB denotes driven
T17820 35412-35414 IN denotes by
T17821 35415-35418 DT denotes the
T17822 35419-35424 JJ denotes human
T17823 35425-35428 NN denotes CD2
T17824 35429-35434 NN denotes locus
T17825 35435-35442 NN denotes control
T17826 35443-35449 NN denotes region
T17827 35450-35451 -LRB- denotes (
T17828 35451-35460 NN denotes CD2-GATA3
T17829 35460-35461 -RRB- denotes )
T17830 35462-35463 -LRB- denotes [
T17831 35463-35465 CD denotes 22
T17832 35465-35466 -RRB- denotes ]
T17833 35466-35467 -COMMA- denotes ,
T17834 35468-35477 VB denotes resulting
T17835 35478-35480 IN denotes in
T17836 35481-35498 NN denotes DO11.10xCD2-GATA3
T17837 35499-35503 NN denotes mice
T17838 35505-35509 NN denotes Mice
T17839 35510-35514 VB denotes used
T17840 35515-35518 IN denotes for
T17841 35519-35530 NN denotes experiments
T17842 35531-35535 VB denotes were
T17843 35536-35547 VB denotes backcrossed
T17844 35548-35550 IN denotes on
T17845 35551-35552 DT denotes a
T17846 35553-35559 NN denotes BALB/c
T17847 35560-35570 NN denotes background
T17848 35571-35574 IN denotes for
T17849 35575-35576 DT denotes a
T17850 35577-35584 NN denotes minimum
T17851 35585-35587 IN denotes of
T17852 35588-35593 CD denotes eight
T17853 35594-35605 NN denotes generations
T17854 35606-35609 CC denotes and
T17855 35610-35614 VB denotes used
T17856 35615-35617 IN denotes at
T17857 35618-35620 DT denotes an
T17858 35621-35624 NN denotes age
T17859 35625-35627 IN denotes of
T17860 35628-35632 CD denotes 8–12
T17861 35633-35635 NN denotes wk
T17862 35637-35641 NN denotes Mice
T17863 35642-35646 VB denotes were
T17864 35647-35653 VB denotes housed
T17865 35654-35659 IN denotes under
T17866 35660-35668 JJ denotes specific
T17867 35669-35682 JJ denotes pathogen-free
T17868 35683-35693 NN denotes conditions
T17869 35694-35697 CC denotes and
T17870 35698-35701 DT denotes all
T17871 35702-35708 NN denotes animal
T17872 35709-35716 NN denotes studies
T17873 35717-35721 VB denotes were
T17874 35722-35731 VB denotes performed
T17875 35732-35741 VB denotes according
T17876 35742-35744 TO denotes to
T17877 35745-35758 JJ denotes institutional
T17878 35759-35762 CC denotes and
T17879 35763-35768 NN denotes state
T17880 35769-35779 NN denotes guidelines
T18081 35782-35791 NN denotes Isolation
T18082 35792-35794 IN denotes of
T18083 35795-35799 JJ denotes CD4+
T18084 35800-35801 NN denotes T
T18085 35802-35807 NN denotes cells
T18086 35809-35813 JJ denotes CD4+
T18087 35814-35815 NN denotes T
T18088 35816-35821 NN denotes cells
T18089 35822-35826 VB denotes were
T18090 35827-35835 VB denotes isolated
T18091 35836-35840 IN denotes from
T18092 35841-35846 NN denotes blood
T18093 35847-35849 IN denotes of
T18094 35850-35857 JJ denotes healthy
T18095 35858-35863 JJ denotes human
T18096 35864-35874 NN denotes volunteers
T18097 35875-35880 VB denotes using
T18098 35881-35884 DT denotes the
T18099 35885-35893 JJ denotes anti-CD4
T18100 35894-35902 JJ denotes magnetic
T18101 35903-35908 NN denotes beads
T18102 35909-35910 -LRB- denotes (
T18103 35910-35915 NNP denotes Dynal
T18104 35915-35916 -COMMA- denotes ,
T18105 35917-35924 NNP denotes Hamburg
T18106 35924-35925 -COMMA- denotes ,
T18107 35926-35933 NNP denotes Germany
T18108 35933-35934 -RRB- denotes )
T18109 35935-35937 IN denotes as
T18110 35938-35948 RB denotes previously
T18111 35949-35958 VB denotes described
T18112 35959-35960 -LRB- denotes [
T18113 35960-35962 CD denotes 60
T18114 35962-35963 -RRB- denotes ]
T18115 35965-35968 DT denotes The
T18116 35969-35975 NN denotes purity
T18117 35976-35978 IN denotes of
T18118 35979-35983 JJ denotes CD4+
T18119 35984-35985 NN denotes T
T18120 35986-35991 NN denotes cells
T18121 35992-35995 VB denotes was
T18122 35996-36005 RB denotes initially
T18123 36006-36012 VB denotes tested
T18124 36013-36015 IN denotes by
T18125 36016-36020 NN denotes FACS
T18126 36021-36024 CC denotes and
T18127 36025-36028 VB denotes was
T18128 36029-36030 RB denotes ≥
T18129 36031-36033 CD denotes 95
T18130 36033-36034 NN denotes %
T18131 36036-36049 NN denotes Monoclonality
T18132 36050-36052 IN denotes of
T18133 36053-36054 NN denotes T
T18134 36055-36059 NN denotes cell
T18135 36060-36066 NN denotes clones
T18136 36067-36070 VB denotes was
T18137 36071-36080 VB denotes confirmed
T18138 36081-36083 IN denotes by
T18139 36084-36093 JJ denotes TCR-chain
T18140 36094-36101 NN denotes mapping
T18141 36102-36105 CC denotes and
T18142 36106-36109 VB denotes was
T18143 36110-36120 VB denotes identified
T18144 36121-36123 TO denotes to
T18145 36124-36126 VB denotes be
T18146 36127-36133 NN denotes Vbeta8
T18147 36134-36142 JJ denotes positive
T18148 36144-36147 DT denotes The
T18149 36148-36154 NN denotes clones
T18150 36155-36159 VB denotes were
T18151 36160-36173 VB denotes characterized
T18152 36174-36176 IN denotes by
T18153 36177-36181 JJ denotes high
T18154 36182-36186 NN denotes IL-4
T18155 36187-36196 NN denotes secretion
T18793 37783-37792 JJ denotes Inducible
T18794 37793-37799 JJ denotes murine
T18795 37800-37804 NN denotes Treg
T18796 37805-37812 NN denotes culture
T18797 37814-37819 JJ denotes Naive
T18798 37820-37824 JJ denotes CD4+
T18799 37825-37826 NN denotes T
T18800 37827-37832 NN denotes cells
T18801 37833-37834 -LRB- denotes (
T18802 37834-37838 JJ denotes CD4+
T18803 37838-37839 -COMMA- denotes ,
T18804 37840-37846 JJ denotes CD62L+
T18805 37846-37847 -COMMA- denotes ,
T18806 37848-37851 CC denotes and
T18807 37852-37857 NN denotes CD25–
T18808 37857-37858 -RRB- denotes )
T18809 37859-37863 VB denotes were
T18810 37864-37872 VB denotes isolated
T18811 37873-37877 IN denotes from
T18812 37878-37884 VB denotes pooled
T18813 37885-37890 NN denotes lymph
T18814 37891-37896 NN denotes nodes
T18815 37897-37900 CC denotes and
T18816 37901-37908 NN denotes spleens
T18817 37909-37911 IN denotes by
T18818 37912-37916 NN denotes FACS
T18819 37917-37918 -LRB- denotes (
T18820 37918-37922 NNP denotes FACS
T18821 37923-37927 NNP denotes Aria
T18822 37927-37928 -COMMA- denotes ,
T18823 37929-37931 NNP denotes BD
T18824 37932-37943 NNP denotes Biosciences
T18825 37943-37944 -RRB- denotes )
T18826 37946-37947 CD denotes 5
T18827 37948-37949 IN denotes ×
T18828 37950-37953 CD denotes 105
T18829 37954-37955 NN denotes T
T18830 37956-37961 NN denotes cells
T18831 37962-37966 VB denotes were
T18832 37967-37978 VB denotes co-cultured
T18833 37979-37983 IN denotes with
T18834 37984-37987 CD denotes 2.5
T18835 37988-37989 -COLON- denotes ×
T18836 37990-37993 CD denotes 104
T18837 37994-37998 NN denotes bone
T18838 37999-38013 VB denotes marrow–derived
T18839 38014-38023 JJ denotes dendritic
T18840 38024-38029 NN denotes cells
T18841 38030-38031 -LRB- denotes [
T18842 38031-38033 CD denotes 62
T18843 38033-38034 -RRB- denotes ]
T18844 38035-38038 CC denotes and
T18845 38039-38043 CD denotes 0.01
T18846 38044-38049 NN denotes μg/ml
T18847 38050-38060 NN denotes OVA323–339
T18848 38061-38068 NN denotes peptide
T18849 38069-38070 -LRB- denotes (
T18850 38070-38077 NN denotes Ansynth
T18851 38077-38078 -RRB- denotes )
T18852 38079-38081 IN denotes in
T18853 38082-38085 DT denotes the
T18854 38086-38094 NN denotes presence
T18855 38095-38097 CC denotes or
T18856 38098-38105 NN denotes absence
T18857 38106-38108 IN denotes of
T18858 38109-38117 JJ denotes 20-ng/μl
T18859 38118-38126 NN denotes rhTGF-ß1
T18860 38127-38128 -LRB- denotes (
T18861 38128-38137 NN denotes Peprotech
T18862 38137-38138 -RRB- denotes )
T18863 38139-38141 IN denotes in
T18864 38142-38149 JJ denotes 48-well
T18865 38150-38156 NN denotes plates
T18866 38158-38163 IN denotes After
T18867 38164-38165 CD denotes 4
T18868 38166-38167 NN denotes d
T18869 38167-38168 -COMMA- denotes ,
T18870 38169-38174 NN denotes cells
T18871 38175-38179 VB denotes were
T18872 38180-38189 VB denotes harvested
T18873 38190-38193 CC denotes and
T18874 38194-38202 VB denotes analyzed
T18875 38203-38206 IN denotes for
T18876 38207-38220 JJ denotes intracellular
T18877 38221-38226 NN denotes FOXP3
T18878 38227-38237 NN denotes expression
T18879 38238-38240 IN denotes by
T18880 38241-38245 NN denotes FACS
T18881 38246-38248 CC denotes or
T18882 38249-38253 NN denotes gene
T18883 38254-38264 NN denotes expression
T18884 38265-38267 IN denotes by
T18885 38268-38280 JJ denotes quantitative
T18886 38281-38287 NN denotes RT-PCR
T19113 38290-38292 FW denotes In
T19114 38293-38298 FW denotes vitro
T19115 38299-38300 NN denotes T
T19116 38301-38305 NN denotes cell
T19117 38306-38321 NN denotes differentiation
T19118 38323-38327 JJ denotes CD4+
T19119 38328-38335 JJ denotes CD45RA+
T19120 38336-38355 JJ denotes magnetically-sorted
T19121 38356-38357 -LRB- denotes (
T19122 38357-38363 NN denotes CD45RO
T19123 38364-38373 NN denotes depletion
T19124 38373-38374 -COMMA- denotes ,
T19125 38375-38379 NN denotes MACS
T19126 38379-38380 -COMMA- denotes ,
T19127 38381-38390 VB denotes according
T19128 38391-38393 TO denotes to
T19129 38394-38397 DT denotes the
T19130 38398-38406 NN denotes protocol
T19131 38407-38409 IN denotes of
T19132 38410-38413 DT denotes the
T19133 38414-38426 NN denotes manufacturer
T19134 38426-38427 -RRB- denotes )
T19135 38428-38433 NN denotes cells
T19136 38434-38438 VB denotes were
T19137 38439-38449 VB denotes stimulated
T19138 38450-38454 IN denotes with
T19139 38455-38466 VB denotes immobilized
T19140 38467-38478 JJ denotes plate-bound
T19141 38479-38487 NN denotes anti-CD3
T19142 38488-38489 -LRB- denotes (
T19143 38489-38490 CD denotes 1
T19144 38491-38496 NN denotes μg/ml
T19145 38496-38497 -COMMA- denotes ,
T19146 38498-38502 NN denotes Okt3
T19147 38502-38503 -COMMA- denotes ,
T19148 38504-38508 NN denotes IgG1
T19149 38508-38509 -RRB- denotes )
T19150 38510-38513 CC denotes and
T19151 38514-38523 JJ denotes anti-CD28
T19152 38524-38525 -LRB- denotes (
T19153 38525-38526 CD denotes 2
T19154 38527-38532 NN denotes μg/ml
T19155 38532-38533 -RRB- denotes )
T19156 38534-38536 IN denotes in
T19157 38537-38540 NN denotes Th1
T19158 38541-38551 NN denotes conditions
T19159 38551-38552 -COLON- denotes :
T19160 38553-38555 CD denotes 25
T19161 38556-38561 NN denotes ng/ml
T19162 38562-38567 NN denotes IL-12
T19163 38567-38568 -COMMA- denotes ,
T19164 38569-38570 CD denotes 5
T19165 38571-38576 NN denotes μg/ml
T19166 38577-38586 NN denotes anti-IL-4
T19167 38587-38588 -LRB- denotes (
T19168 38588-38589 NN denotes R
T19169 38589-38590 CC denotes &
T19170 38590-38591 NN denotes D
T19171 38592-38599 NN denotes systems
T19172 38599-38600 -RRB- denotes )
T19173 38600-38601 -COLON- denotes ;
T19174 38602-38604 IN denotes in
T19175 38605-38608 NN denotes Th2
T19176 38609-38619 NN denotes conditions
T19177 38619-38620 -COLON- denotes :
T19178 38621-38623 CD denotes 25
T19179 38624-38629 NN denotes ng/ml
T19180 38630-38634 NN denotes IL-4
T19181 38634-38635 -COMMA- denotes ,
T19182 38636-38637 CD denotes 5
T19183 38638-38643 NN denotes μg/ml
T19184 38644-38654 JJ denotes anti-IFN-γ
T19185 38654-38655 -COMMA- denotes ,
T19186 38656-38657 CD denotes 5
T19187 38658-38663 NN denotes μg/ml
T19188 38664-38674 NN denotes anti-IL-12
T19189 38675-38676 -LRB- denotes (
T19190 38676-38677 NN denotes R
T19191 38677-38678 CC denotes &
T19192 38678-38679 NN denotes D
T19193 38680-38687 NN denotes systems
T19194 38687-38688 -RRB- denotes )
T19195 38688-38689 -COLON- denotes ;
T19196 38690-38692 CC denotes or
T19197 38693-38695 IN denotes in
T19198 38696-38700 NNP denotes Treg
T19199 38701-38711 NN denotes conditions
T19200 38711-38712 -COLON- denotes :
T19201 38713-38715 CD denotes 10
T19202 38716-38721 NN denotes ng/ml
T19203 38722-38727 NN denotes TGF-β
T19204 38727-38728 -COMMA- denotes ,
T19205 38729-38730 CD denotes 5
T19206 38731-38736 NN denotes μg/ml
T19207 38737-38747 JJ denotes anti-IFN-γ
T19208 38747-38748 -COMMA- denotes ,
T19209 38749-38750 CD denotes 5
T19210 38751-38756 NN denotes μg/ml
T19211 38757-38767 NN denotes anti-IL-12
T19212 38767-38768 -COMMA- denotes ,
T19213 38769-38770 CD denotes 5
T19214 38771-38776 NN denotes μg/ml
T19215 38777-38786 NN denotes anti-IL-4
T19216 38788-38801 VB denotes Proliferating
T19217 38802-38807 NN denotes cells
T19218 38808-38812 VB denotes were
T19219 38813-38821 VB denotes expanded
T19220 38822-38824 IN denotes in
T19221 38825-38831 NN denotes medium
T19222 38832-38842 VB denotes containing
T19223 38843-38847 NN denotes IL-2
T19224 38848-38849 -LRB- denotes (
T19225 38849-38851 CD denotes 30
T19226 38852-38857 NN denotes ng/ml
T19227 38857-38858 -RRB- denotes )
T19537 38960-38964 IN denotes into
T19538 38965-38968 DT denotes the
T19539 38969-38973 NN denotes pGL3
T19540 38974-38979 JJ denotes basic
T19541 38980-38986 NN denotes vector
T19542 38987-38988 -LRB- denotes (
T19543 38988-38995 NNP denotes Promega
T19544 38996-39003 NNP denotes Biotech
T19545 39003-39004 -RRB- denotes )
T19546 39005-39007 TO denotes to
T19547 39008-39016 VB denotes generate
T19548 39017-39020 DT denotes the
T19549 39021-39025 NN denotes pGL3
T19550 39026-39031 NN denotes FOXP3
T19551 39032-39041 CD denotes −511/+176
T19552 39042-39043 -LRB- denotes [
T19553 39043-39045 CD denotes 24
T19554 39045-39046 -RRB- denotes ]
T19555 39048-39061 JJ denotes Site-directed
T19556 39062-39073 NN denotes mutagenesis
T19557 39074-39076 IN denotes in
T19558 39077-39080 DT denotes the
T19559 39081-39086 NN denotes FOXP3
T19560 39087-39095 NN denotes promoter
T19561 39096-39102 NN denotes region
T19562 39103-39107 VB denotes were
T19563 39108-39118 VB denotes introduced
T19564 39119-39124 VB denotes using
T19565 39125-39128 DT denotes the
T19566 39129-39140 NN denotes QuickChange
T19567 39141-39144 NN denotes kit
T19568 39145-39146 -LRB- denotes (
T19569 39146-39156 NN denotes Stratagene
T19570 39156-39157 -RRB- denotes )
T19571 39157-39158 -COMMA- denotes ,
T19572 39159-39168 VB denotes according
T19573 39169-39171 TO denotes to
T19574 39172-39175 DT denotes the
T19575 39176-39188 NN denotes manufacturer
T19576 39188-39190 POS denotes 's
T19577 39191-39203 NN denotes instructions
T19578 39205-39208 DT denotes The
T19579 39209-39218 VB denotes following
T19580 39219-39225 NN denotes primer
T19581 39226-39229 CC denotes and
T19582 39230-39233 PRP-DOLLAR- denotes its
T19583 39234-39247 JJ denotes complementary
T19584 39248-39254 NN denotes strand
T19585 39255-39259 VB denotes were
T19586 39260-39264 VB denotes used
T19587 39264-39265 -COLON- denotes :
T19588 39266-39269 NN denotes GTT
T19589 39270-39273 NN denotes TCT
T19590 39274-39277 NN denotes CAT
T19591 39278-39281 NNP denotes GAG
T19592 39282-39285 NNP denotes CCC
T19593 39286-39289 NN denotes TAT
T19594 39290-39293 NNP denotes TAA
T19595 39294-39297 NNP denotes GTC
T19596 39298-39301 NNP denotes ATT
T19597 39302-39305 NNP denotes CTT
T19598 39306-39309 NNP denotes ACC
T19599 39310-39313 NN denotes TCT
T19600 39314-39317 NNP denotes CAC
T19601 39318-39321 NNP denotes CTC
T19602 39322-39325 NNP denotes TGT
T19603 39326-39329 NNP denotes GGT
T19604 39330-39332 NNP denotes GA
T19770 39335-39348 NN denotes Transfections
T19771 39349-39352 CC denotes and
T19772 39353-39361 NN denotes reporter
T19773 39362-39366 NN denotes gene
T19774 39367-39373 NN denotes assays
T19775 39375-39376 NN denotes T
T19776 39377-39382 NN denotes cells
T19777 39383-39387 VB denotes were
T19778 39388-39394 VB denotes rested
T19779 39395-39397 IN denotes in
T19780 39398-39408 JJ denotes serum-free
T19781 39409-39414 NN denotes AIM-V
T19782 39415-39421 NN denotes medium
T19783 39422-39423 -LRB- denotes (
T19784 39423-39427 NNP denotes Life
T19785 39428-39440 NNP denotes Technologies
T19786 39440-39441 -RRB- denotes )
T19787 39442-39451 RB denotes overnight
T19788 39453-39456 CD denotes 3.5
T19789 39457-39459 NN denotes μg
T19790 39460-39462 IN denotes of
T19791 39463-39466 DT denotes the
T19792 39467-39472 NN denotes FOXP3
T19793 39473-39481 NN denotes promoter
T19794 39482-39492 NN denotes luciferase
T19795 39493-39501 NN denotes reporter
T19796 39502-39508 NN denotes vector
T19797 39509-39512 CC denotes and
T19798 39513-39516 CD denotes 0.5
T19799 39517-39519 NN denotes μg
T19800 39520-39527 NN denotes phRL-TK
T19801 39528-39532 VB denotes were
T19802 39533-39538 VB denotes added
T19803 39539-39541 TO denotes to
T19804 39542-39543 CD denotes 3
T19805 39544-39545 SYM denotes ×
T19806 39546-39549 CD denotes 106
T19807 39550-39554 JJ denotes CD4+
T19808 39555-39556 NN denotes T
T19809 39557-39562 NN denotes cells
T19810 39563-39574 VB denotes resuspended
T19811 39575-39577 IN denotes in
T19812 39578-39581 CD denotes 100
T19813 39582-39584 NN denotes μL
T19814 39585-39587 IN denotes of
T19815 39588-39600 NN denotes Nucleofector
T19816 39601-39609 NN denotes solution
T19817 39610-39611 -LRB- denotes (
T19818 39611-39616 NNP denotes Amaxa
T19819 39617-39627 NNP denotes Biosystems
T19820 39627-39628 -RRB- denotes )
T19821 39629-39632 CC denotes and
T19822 39633-39647 VB denotes electroporated
T19823 39648-39653 VB denotes using
T19824 39654-39657 DT denotes the
T19825 39658-39662 NN denotes U-15
T19826 39663-39670 NN denotes program
T19827 39671-39673 IN denotes of
T19828 39674-39677 DT denotes the
T19829 39678-39691 NNP denotes Nucleofector.
T19830 39692-39697 IN denotes After
T19831 39698-39699 DT denotes a
T19832 39700-39704 JJ denotes 24-h
T19833 39705-39712 NN denotes culture
T19834 39713-39715 IN denotes in
T19835 39716-39726 JJ denotes serum-free
T19836 39727-39737 NN denotes conditions
T19837 39738-39741 CC denotes and
T19838 39742-39749 NN denotes stimuli
T19839 39750-39752 IN denotes as
T19840 39753-39762 VB denotes indicated
T19841 39763-39765 IN denotes in
T19842 39766-39769 DT denotes the
T19843 39770-39777 NN denotes figures
T19844 39777-39778 -COMMA- denotes ,
T19845 39779-39789 NN denotes luciferase
T19846 39790-39798 NN denotes activity
T19847 39799-39802 VB denotes was
T19848 39803-39811 VB denotes measured
T19849 39812-39814 IN denotes by
T19850 39815-39818 DT denotes the
T19851 39819-39823 JJ denotes dual
T19852 39824-39834 NN denotes luciferase
T19853 39835-39840 NN denotes assay
T19854 39841-39847 NN denotes system
T19855 39848-39849 -LRB- denotes (
T19856 39849-39856 NNP denotes Promega
T19857 39857-39864 NNP denotes Biotech
T19858 39864-39865 -RRB- denotes )
T19859 39866-39875 VB denotes according
T19860 39876-39878 TO denotes to
T19861 39879-39882 DT denotes the
T19862 39883-39895 NN denotes manufacturer
T19863 39895-39897 POS denotes 's
T19864 39898-39910 NN denotes instructions
T19865 39912-39916 NN denotes Data
T19866 39917-39921 VB denotes were
T19867 39922-39932 VB denotes normalized
T19868 39933-39935 IN denotes by
T19869 39936-39939 DT denotes the
T19870 39940-39948 NN denotes activity
T19871 39949-39951 IN denotes of
T19872 39952-39959 NN denotes renilla
T19873 39960-39970 NN denotes luciferase
T20080 40154-40158 IN denotes with
T20081 40159-40165 NNP denotes TaqMan
T20082 40166-40187 NN denotes reverse-transcription
T20083 40188-40196 NN denotes reagents
T20084 40197-40198 -LRB- denotes (
T20085 40198-40205 NNP denotes Applied
T20086 40206-40216 NNP denotes Biosystems
T20087 40216-40217 -RRB- denotes )
T20088 40218-40222 IN denotes with
T20089 40223-40229 JJ denotes random
T20090 40230-40238 NN denotes hexamers
T20091 40239-40248 VB denotes according
T20092 40249-40251 TO denotes to
T20093 40252-40255 DT denotes the
T20094 40256-40268 NN denotes manufacturer
T20095 40268-40270 POS denotes 's
T20096 40271-40279 NN denotes protocol
T20175 40282-40293 JJ denotes Recombinant
T20176 40294-40297 NN denotes TAT
T20177 40298-40306 NN denotes proteins
T20178 40308-40311 DT denotes The
T20179 40312-40317 NN denotes cDNAs
T20180 40318-40326 VB denotes encoding
T20181 40327-40332 NN denotes GATA3
T20182 40333-40340 NN denotes protein
T20183 40341-40343 CC denotes or
T20184 40344-40347 DT denotes the
T20185 40348-40357 VB denotes truncated
T20186 40358-40363 NN denotes GATA3
T20187 40364-40365 -LRB- denotes (
T20188 40365-40372 VB denotes lacking
T20189 40373-40376 DT denotes the
T20190 40377-40380 CD denotes two
T20191 40381-40385 NN denotes zinc
T20192 40386-40393 NN denotes fingers
T20193 40393-40394 -RRB- denotes )
T20194 40395-40399 VB denotes were
T20195 40400-40406 VB denotes cloned
T20196 40407-40409 IN denotes in
T20197 40410-40415 NN denotes frame
T20198 40416-40420 IN denotes into
T20199 40421-40423 DT denotes an
T20200 40424-40434 NN denotes expression
T20201 40435-40441 NN denotes vector
T20202 40442-40447 IN denotes along
T20203 40448-40452 IN denotes with
T20204 40453-40456 DT denotes the
T20205 40457-40460 NN denotes TAT
T20206 40461-40469 NN denotes sequence
T20207 40470-40472 IN denotes as
T20208 40473-40483 RB denotes previously
T20209 40484-40493 VB denotes described
T20210 40494-40495 -LRB- denotes [
T20211 40495-40497 CD denotes 63
T20212 40497-40498 -RRB- denotes ]
T20213 40500-40508 NN denotes Proteins
T20214 40509-40513 VB denotes were
T20215 40514-40523 VB denotes expressed
T20216 40524-40526 IN denotes in
T20217 40527-40531 NN denotes BL21
T20218 40532-40536 NN denotes Star
T20219 40537-40538 -LRB- denotes (
T20220 40538-40541 NN denotes DE3
T20221 40541-40542 -RRB- denotes )
T20222 40542-40547 NN denotes pLysS
T20223 40548-40549 -LRB- denotes (
T20224 40549-40559 NN denotes Invitrogen
T20225 40559-40560 -RRB- denotes )
T20226 40561-40564 CC denotes and
T20227 40565-40572 NN denotes lysates
T20228 40573-40577 VB denotes were
T20229 40578-40586 VB denotes purified
T20230 40587-40589 IN denotes by
T20231 40590-40602 JJ denotes Ni2+-chelate
T20232 40603-40609 NN denotes column
T20233 40610-40624 NN denotes chromatography
T20234 40626-40630 DT denotes Both
T20235 40631-40641 JJ denotes TAT-linked
T20236 40642-40650 NN denotes proteins
T20237 40651-40655 VB denotes were
T20238 40656-40660 JJ denotes more
T20239 40661-40665 IN denotes than
T20240 40666-40668 CD denotes 95
T20241 40668-40669 NN denotes %
T20242 40670-40674 JJ denotes pure
T20243 40674-40675 -COMMA- denotes ,
T20244 40676-40681 VB denotes based
T20245 40682-40684 IN denotes on
T20246 40685-40694 NNP denotes Coomassie
T20247 40695-40699 JJ denotes blue
T20248 40700-40708 NN denotes staining
T20249 40709-40711 IN denotes of
T20250 40712-40718 NN denotes sodium
T20251 40719-40728 NN denotes disulfate
T20252 40729-40739 NN denotes acrylamide
T20253 40740-40744 NN denotes gels
T20445 40747-40756 NN denotes TAT-GATA3
T20446 40757-40769 NN denotes transduction
T20447 40771-40782 JJ denotes CD4+CD45RA+
T20448 40783-40788 NN denotes cells
T20449 40789-40793 VB denotes were
T20450 40794-40802 VB denotes cultured
T20451 40803-40805 IN denotes in
T20452 40806-40810 NN denotes AIMV
T20453 40811-40817 NN denotes medium
T20454 40818-40821 CC denotes and
T20455 40822-40832 VB denotes transduced
T20456 40833-40837 IN denotes with
T20457 40838-40840 CD denotes 20
T20458 40841-40843 NN denotes nM
T20459 40843-40844 -COMMA- denotes ,
T20460 40845-40847 CD denotes 10
T20461 40848-40850 NN denotes nM
T20462 40850-40851 -COMMA- denotes ,
T20463 40852-40854 CC denotes or
T20464 40855-40858 CD denotes 500
T20465 40859-40861 NN denotes nM
T20466 40862-40864 IN denotes of
T20467 40865-40876 JJ denotes full-length
T20468 40877-40879 CC denotes or
T20469 40880-40889 VB denotes truncated
T20470 40890-40895 NN denotes GATA3
T20471 40896-40900 IN denotes over
T20472 40901-40904 DT denotes the
T20473 40905-40911 NN denotes course
T20474 40912-40914 IN denotes of
T20475 40915-40916 CD denotes 4
T20476 40917-40918 NN denotes h
T20477 40920-40925 IN denotes After
T20478 40926-40927 CD denotes 4
T20479 40928-40929 NN denotes h
T20480 40929-40930 -COMMA- denotes ,
T20481 40931-40934 DT denotes the
T20482 40935-40940 NN denotes cells
T20483 40941-40945 VB denotes were
T20484 40946-40952 VB denotes washed
T20485 40953-40956 CC denotes and
T20486 40957-40966 VB denotes activated
T20487 40967-40971 IN denotes with
T20488 40972-40979 JJ denotes soluble
T20489 40980-40988 NN denotes anti-CD3
T20490 40989-40992 CC denotes and
T20491 40993-41002 NN denotes anti-CD28
T20492 41003-41006 CC denotes and
T20493 41007-41012 NN denotes TGF-β
T20494 41013-41014 -LRB- denotes (
T20495 41014-41016 CD denotes 10
T20496 41017-41022 NN denotes ng/ml
T20497 41022-41023 -RRB- denotes )
T20498 41025-41029 DT denotes Each
T20499 41030-41033 NN denotes day
T20500 41033-41034 -COMMA- denotes ,
T20501 41035-41038 DT denotes the
T20502 41039-41042 NN denotes TAT
T20503 41043-41051 NN denotes proteins
T20504 41052-41056 VB denotes were
T20505 41057-41064 RB denotes freshly
T20506 41065-41070 VB denotes added
T20507 41071-41073 TO denotes to
T20508 41074-41077 DT denotes the
T20509 41078-41084 NN denotes medium
T20510 41086-41091 NN denotes FOXP3
T20511 41092-41102 NN denotes expression
T20512 41103-41106 VB denotes was
T20513 41107-41115 VB denotes measured
T20514 41116-41121 IN denotes after
T20515 41122-41123 CD denotes 5
T20516 41124-41125 NN denotes d
T20517 41126-41128 IN denotes by
T20518 41129-41142 JJ denotes intracellular
T20519 41143-41151 NN denotes staining
T20715 41154-41167 JJ denotes Intracellular
T20716 41168-41176 NN denotes cytokine
T20717 41177-41185 NN denotes staining
T20718 41187-41188 NN denotes T
T20719 41189-41194 NN denotes cells
T20720 41195-41199 VB denotes were
T20721 41200-41210 VB denotes stimulated
T20722 41211-41215 IN denotes with
T20723 41216-41217 CD denotes 2
T20724 41218-41219 SYM denotes ×
T20725 41220-41224 CD denotes 10−7
T20726 41225-41226 NN denotes M
T20727 41227-41230 NN denotes PMA
T20728 41231-41234 CC denotes and
T20729 41235-41236 CD denotes 1
T20730 41237-41242 NN denotes μg/ml
T20731 41243-41245 IN denotes of
T20732 41246-41255 NN denotes ionomycin
T20733 41256-41257 -LRB- denotes (
T20734 41257-41262 NNP denotes Sigma
T20735 41263-41272 NNP denotes Chemicals
T20736 41272-41273 -RRB- denotes )
T20737 41274-41277 IN denotes for
T20738 41278-41279 CD denotes 4
T20739 41280-41281 NN denotes h
T20740 41283-41286 DT denotes The
T20741 41287-41296 VB denotes following
T20742 41297-41300 NN denotes mAb
T20743 41301-41304 VB denotes was
T20744 41305-41309 VB denotes used
T20745 41309-41310 -COLON- denotes :
T20746 41311-41323 JJ denotes anti-IL-4-PE
T20747 41324-41325 -LRB- denotes (
T20748 41325-41330 NN denotes 8D4–8
T20749 41330-41331 -COMMA- denotes ,
T20750 41332-41334 NN denotes BD
T20751 41334-41335 -RRB- denotes )
T20752 41337-41344 VB denotes Matched
T20753 41345-41352 NN denotes isotype
T20754 41353-41361 NN denotes controls
T20755 41362-41366 VB denotes were
T20756 41367-41371 VB denotes used
T20757 41372-41374 IN denotes at
T20758 41375-41378 DT denotes the
T20759 41379-41383 JJ denotes same
T20760 41384-41391 NN denotes protein
T20761 41392-41405 NN denotes concentration
T20762 41406-41408 IN denotes as
T20763 41409-41412 DT denotes the
T20764 41413-41423 JJ denotes respective
T20765 41424-41434 NN denotes antibodies
T20766 41436-41446 JJ denotes Four-color
T20767 41447-41451 NN denotes FACS
T20768 41452-41455 VB denotes was
T20769 41456-41465 VB denotes performed
T20770 41466-41471 VB denotes using
T20771 41472-41474 DT denotes an
T20772 41475-41480 NN denotes EPICS
T20773 41481-41487 NN denotes XL-MCL
T20774 41488-41489 -LRB- denotes (
T20775 41489-41496 NNP denotes Beckman
T20776 41497-41504 NNP denotes Coulter
T20777 41504-41505 -RRB- denotes )
T20778 41506-41511 VB denotes using
T20779 41512-41515 DT denotes the
T20780 41516-41524 NN denotes software
T20781 41525-41531 NN denotes Expo32
T20782 41532-41539 NN denotes version
T20783 41540-41543 IN denotes for
T20784 41544-41548 NN denotes data
T20785 41549-41560 NN denotes acquisition
T20786 41561-41564 CC denotes and
T20787 41565-41575 NN denotes evaluation
T20968 41578-41582 NN denotes Flow
T20969 41583-41592 NN denotes cytometry
T20970 41594-41597 IN denotes For
T20971 41598-41606 NN denotes analysis
T20972 41607-41609 IN denotes of
T20973 41610-41615 NN denotes FOXP3
T20974 41616-41626 NN denotes expression
T20975 41627-41629 IN denotes at
T20976 41630-41633 DT denotes the
T20977 41634-41645 JJ denotes single-cell
T20978 41646-41651 NN denotes level
T20979 41651-41652 -COMMA- denotes ,
T20980 41653-41658 NN denotes cells
T20981 41659-41663 VB denotes were
T20982 41664-41669 RB denotes first
T20983 41670-41677 VB denotes stained
T20984 41678-41682 IN denotes with
T20985 41683-41686 DT denotes the
T20986 41687-41697 JJ denotes monoclonal
T20987 41698-41706 NN denotes antibody
T20988 41707-41711 NN denotes CD25
T20989 41712-41713 -LRB- denotes (
T20990 41713-41720 NNP denotes Beckman
T20991 41721-41728 NNP denotes Coulter
T20992 41728-41729 -RRB- denotes )
T20993 41729-41730 -COMMA- denotes ,
T20994 41731-41734 CC denotes and
T20995 41735-41740 IN denotes after
T20996 41741-41749 NN denotes fixation
T20997 41750-41753 CC denotes and
T20998 41754-41770 NN denotes permeabilization
T20999 41770-41771 -COMMA- denotes ,
T21000 41772-41777 NN denotes cells
T21001 41778-41782 VB denotes were
T21002 41783-41792 VB denotes incubated
T21003 41793-41797 IN denotes with
T21004 41798-41811 JJ denotes PE-conjugated
T21005 41812-41822 JJ denotes monoclonal
T21006 41823-41831 NN denotes antibody
T21007 41832-41838 NN denotes PCH101
T21008 41839-41840 -LRB- denotes (
T21009 41840-41850 JJ denotes anti-human
T21010 41851-41856 NN denotes FOXP3
T21011 41856-41857 -COLON- denotes ;
T21012 41858-41869 NN denotes eBioscience
T21013 41869-41870 -RRB- denotes )
T21014 41871-41876 VB denotes based
T21015 41877-41879 IN denotes on
T21016 41880-41883 DT denotes the
T21017 41884-41896 NN denotes manufacturer
T21018 41896-41898 POS denotes 's
T21019 41899-41914 NN denotes recommendations
T21020 41915-41918 CC denotes and
T21021 41919-41928 VB denotes subjected
T21022 41929-41931 TO denotes to
T21023 41932-41936 NN denotes FACS
T21024 41937-41938 -LRB- denotes (
T21025 41938-41943 NN denotes EPICS
T21026 41944-41950 NN denotes XL-MCL
T21027 41950-41951 -RRB- denotes )
T21028 41953-41956 IN denotes For
T21029 41957-41961 NN denotes cell
T21030 41962-41969 NN denotes surface
T21031 41970-41976 NN denotes marker
T21032 41977-41985 NN denotes staining
T21033 41985-41986 -COMMA- denotes ,
T21034 41987-41992 NN denotes cells
T21035 41993-41997 VB denotes were
T21036 41998-42007 VB denotes incubated
T21037 42008-42011 IN denotes for
T21038 42012-42014 CD denotes 20
T21039 42015-42018 NN denotes min
T21040 42019-42021 IN denotes at
T21041 42022-42025 NN denotes 4°C
T21042 42026-42028 IN denotes in
T21043 42029-42037 NN denotes staining
T21044 42038-42044 NN denotes buffer
T21045 42045-42049 IN denotes with
T21046 42050-42053 DT denotes the
T21047 42054-42063 VB denotes following
T21048 42064-42074 NN denotes antibodies
T21049 42074-42075 -COLON- denotes :
T21050 42076-42089 NN denotes anti–CD152-PE
T21051 42090-42091 -LRB- denotes (
T21052 42091-42097 NN denotes CTLA-4
T21053 42097-42098 -COLON- denotes ;
T21054 42099-42101 NN denotes BD
T21055 42101-42102 -RRB- denotes )
T21056 42102-42103 -COMMA- denotes ,
T21057 42104-42113 NN denotes anti–PD-1
T21058 42114-42115 -LRB- denotes (
T21059 42115-42127 NN denotes eBiosciences
T21060 42127-42128 -RRB- denotes )
T21061 42128-42129 -COMMA- denotes ,
T21062 42130-42139 NN denotes anti-GITR
T21063 42140-42141 -LRB- denotes (
T21064 42141-42142 NNP denotes R
T21065 42143-42144 CC denotes &
T21066 42145-42146 NNP denotes D
T21067 42147-42154 NNP denotes Systems
T21068 42154-42155 -RRB- denotes )
T21069 42155-42156 -COMMA- denotes ,
T21070 42157-42166 NN denotes anti-CD69
T21071 42167-42168 -LRB- denotes (
T21072 42168-42175 NNP denotes Beckman
T21073 42176-42183 NNP denotes Coulter
T21074 42183-42184 -RRB- denotes )
T21075 42184-42185 -COMMA- denotes ,
T21076 42186-42196 NN denotes anti-CD103
T21077 42197-42198 -LRB- denotes (
T21078 42198-42212 NN denotes DakoCytomation
T21079 42212-42213 -RRB- denotes )
T21080 42213-42214 -COMMA- denotes ,
T21081 42215-42225 NN denotes anti-CD62L
T21082 42226-42227 -LRB- denotes (
T21083 42227-42234 NNP denotes Beckman
T21084 42235-42242 NNP denotes Coulter
T21085 42242-42243 -RRB- denotes )
T21086 42243-42244 -COMMA- denotes ,
T21087 42245-42247 CC denotes or
T21088 42248-42259 JJ denotes anti-HLA-DR
T21089 42260-42261 -LRB- denotes (
T21090 42261-42268 NNP denotes Beckman
T21091 42269-42276 NNP denotes Coulter
T21092 42276-42277 -RRB- denotes )
T21093 42279-42282 DT denotes The
T21094 42283-42291 NN denotes controls
T21095 42292-42296 VB denotes were
T21096 42297-42301 NN denotes FITC
T21097 42301-42302 -COMMA- denotes ,
T21098 42303-42305 NN denotes PE
T21099 42305-42306 -COMMA- denotes ,
T21100 42307-42309 CC denotes or
T21101 42310-42324 JJ denotes ECD-conjugated
T21102 42325-42330 NN denotes mouse
T21103 42331-42335 NN denotes IgG1
T21104 42336-42338 CC denotes or
T21105 42339-42342 NN denotes rat
T21106 42343-42348 NN denotes IgG2a
T21107 42350-42353 IN denotes For
T21108 42354-42362 NN denotes staining
T21109 42363-42365 IN denotes of
T21110 42366-42371 NN denotes mouse
T21111 42372-42377 NN denotes cells
T21112 42377-42378 -COMMA- denotes ,
T21113 42379-42382 DT denotes the
T21114 42383-42392 VB denotes following
T21115 42393-42397 NN denotes mAbs
T21116 42398-42402 IN denotes from
T21117 42403-42405 NN denotes BD
T21118 42406-42417 NN denotes Biosciences
T21119 42418-42422 VB denotes were
T21120 42423-42427 VB denotes used
T21121 42428-42437 VB denotes following
T21122 42438-42446 JJ denotes standard
T21123 42447-42457 NN denotes techniques
T21124 42458-42460 IN denotes as
T21125 42461-42470 VB denotes described
T21126 42471-42476 IN denotes above
T21127 42476-42477 -COLON- denotes :
T21128 42478-42486 NN denotes anti-CD3
T21129 42486-42487 -COMMA- denotes ,
T21130 42488-42496 NN denotes anti-CD4
T21131 42496-42497 -COMMA- denotes ,
T21132 42498-42501 CC denotes and
T21133 42502-42511 NN denotes anti-CD25
T21134 42513-42528 JJ denotes Anti-FcγRII/III
T21135 42529-42537 NN denotes antibody
T21136 42538-42539 -LRB- denotes (
T21137 42539-42544 NN denotes 2.4G2
T21138 42544-42545 -COMMA- denotes ,
T21139 42546-42550 NN denotes ATCC
T21140 42550-42551 -RRB- denotes )
T21141 42552-42555 VB denotes was
T21142 42556-42564 VB denotes included
T21143 42565-42567 IN denotes in
T21144 42568-42571 DT denotes all
T21145 42572-42581 NN denotes stainings
T21146 42582-42584 TO denotes to
T21147 42585-42591 VB denotes reduce
T21148 42592-42603 JJ denotes nonspecific
T21149 42604-42612 NN denotes antibody
T21150 42613-42620 NN denotes binding
T21151 42622-42624 TO denotes To
T21152 42625-42632 VB denotes isolate
T21153 42633-42638 JJ denotes naive
T21154 42639-42645 JJ denotes murine
T21155 42646-42649 NN denotes CD4
T21156 42650-42651 NN denotes T
T21157 42652-42657 NN denotes cells
T21158 42658-42662 IN denotes from
T21159 42663-42669 JJ denotes murine
T21160 42670-42677 NN denotes DO11.10
T21161 42678-42680 CC denotes or
T21162 42681-42698 NN denotes DO11.10xCD2-GATA3
T21163 42699-42700 NN denotes T
T21164 42701-42706 NN denotes cells
T21165 42706-42707 -COMMA- denotes ,
T21166 42708-42713 NN denotes cells
T21167 42714-42718 VB denotes were
T21168 42719-42726 VB denotes stained
T21169 42727-42731 IN denotes with
T21170 42732-42746 NN denotes anti-CD25-FITC
T21171 42746-42747 -COMMA- denotes ,
T21172 42748-42761 NN denotes anti-CD62L-PE
T21173 42761-42762 -COMMA- denotes ,
T21174 42763-42766 CC denotes and
T21175 42767-42779 NN denotes anti-CD4-APC
T21176 42780-42785 JJ denotes prior
T21177 42786-42788 TO denotes to
T21178 42789-42796 NN denotes sorting
T21179 42798-42802 NN denotes Dead
T21180 42803-42808 NN denotes cells
T21181 42809-42813 VB denotes were
T21182 42814-42822 VB denotes excluded
T21183 42823-42827 IN denotes with
T21184 42828-42830 NN denotes 4′
T21185 42830-42831 -DQE- denotes '
T21186 42831-42832 -COMMA- denotes ,
T21187 42832-42858 NN denotes 6-Diamidino-2-phenylindole
T21188 42859-42860 -LRB- denotes (
T21189 42860-42864 NN denotes DAPI
T21190 42864-42865 -RRB- denotes )
T21191 42867-42869 TO denotes To
T21192 42870-42877 VB denotes analyze
T21193 42878-42884 JJ denotes murine
T21194 42885-42890 NN denotes Foxp3
T21195 42891-42901 NN denotes expression
T21196 42902-42904 IN denotes in
T21197 42905-42914 JJ denotes inducible
T21198 42915-42919 NN denotes Treg
T21199 42920-42928 NN denotes cultures
T21200 42928-42929 -COMMA- denotes ,
T21201 42930-42935 NN denotes cells
T21202 42936-42940 VB denotes were
T21203 42941-42948 VB denotes stained
T21204 42949-42964 RB denotes intracellularly
T21205 42965-42969 IN denotes with
T21206 42970-42983 JJ denotes anti-Foxp3-PE
T21207 42984-42993 VB denotes according
T21208 42994-42996 TO denotes to
T21209 42997-43009 NN denotes manufacturer
T21210 43009-43011 POS denotes 's
T21211 43012-43023 NN denotes instruction
T21212 43023-43024 -COMMA- denotes ,
T21213 43025-43027 IN denotes in
T21214 43028-43039 NN denotes conjunction
T21215 43040-43044 IN denotes with
T21216 43045-43057 NN denotes anti-CD4-APC
T21217 43058-43061 CC denotes and
T21218 43062-43071 NN denotes LIVE/DEAD
T21219 43072-43079 JJ denotes fixable
T21220 43080-43084 JJ denotes dead
T21221 43085-43089 NN denotes cell
T21222 43090-43095 NN denotes stain
T21223 43096-43099 NN denotes kit
T21224 43100-43101 -LRB- denotes (
T21225 43101-43111 NN denotes Invitrogen
T21226 43111-43112 -RRB- denotes )
T21227 43113-43115 TO denotes to
T21228 43116-43128 VB denotes discriminate
T21229 43129-43133 JJ denotes live
T21230 43134-43139 NN denotes cells
T21231 43141-43144 DT denotes All
T21232 43145-43155 JJ denotes monoclonal
T21233 43156-43166 NN denotes antibodies
T21234 43167-43170 IN denotes for
T21235 43171-43177 JJ denotes murine
T21236 43178-43182 NN denotes cell
T21237 43183-43192 NN denotes stainings
T21238 43193-43197 VB denotes were
T21239 43198-43207 VB denotes purchased
T21240 43208-43212 IN denotes from
T21241 43213-43224 NN denotes eBioscience
T21242 43225-43227 CC denotes or
T21243 43228-43230 NN denotes BD
T21244 43231-43242 NNP denotes Biosciences
T21790 43245-43252 NN denotes Western
T21791 43253-43261 NN denotes blotting
T21792 43263-43266 IN denotes For
T21793 43267-43272 NN denotes FOXP3
T21794 43273-43281 NN denotes analysis
T21795 43282-43284 IN denotes on
T21796 43285-43288 DT denotes the
T21797 43289-43296 NN denotes protein
T21798 43297-43302 NN denotes level
T21799 43302-43303 -COMMA- denotes ,
T21800 43304-43305 CD denotes 1
T21801 43306-43307 CC denotes ×
T21802 43308-43311 CD denotes 106
T21803 43312-43321 NN denotes CD4+CD25–
T21804 43322-43327 NN denotes cells
T21805 43328-43332 VB denotes were
T21806 43333-43338 VB denotes lysed
T21807 43339-43342 CC denotes and
T21808 43343-43349 VB denotes loaded
T21809 43350-43354 JJ denotes next
T21810 43355-43357 TO denotes to
T21811 43358-43359 DT denotes a
T21812 43360-43372 JJ denotes protein-mass
T21813 43373-43379 NN denotes ladder
T21814 43380-43381 -LRB- denotes (
T21815 43381-43390 NNP denotes Magicmark
T21816 43390-43391 -COMMA- denotes ,
T21817 43392-43402 NN denotes Invitrogen
T21818 43402-43403 -RRB- denotes )
T21819 43404-43406 IN denotes on
T21820 43407-43408 DT denotes a
T21821 43409-43415 NN denotes NuPAGE
T21822 43416-43420 CD denotes 4–12
T21823 43420-43421 NN denotes %
T21824 43422-43430 JJ denotes bis-tris
T21825 43431-43434 NN denotes gel
T21826 43435-43436 -LRB- denotes (
T21827 43436-43446 NN denotes Invitrogen
T21828 43446-43447 -RRB- denotes )
T21829 43449-43452 DT denotes The
T21830 43453-43461 NN denotes proteins
T21831 43462-43466 VB denotes were
T21832 43467-43481 VB denotes electroblotted
T21833 43482-43486 IN denotes onto
T21834 43487-43488 DT denotes a
T21835 43489-43493 NN denotes PVDF
T21836 43494-43502 NN denotes membrane
T21837 43503-43504 -LRB- denotes (
T21838 43504-43512 NNP denotes Amersham
T21839 43513-43517 NNP denotes Life
T21840 43518-43525 NNP denotes Science
T21841 43525-43526 -RRB- denotes )
T21842 43528-43538 JJ denotes Unspecific
T21843 43539-43546 NN denotes binding
T21844 43547-43550 VB denotes was
T21845 43551-43558 VB denotes blocked
T21846 43559-43563 IN denotes with
T21847 43564-43567 NN denotes BSA
T21848 43567-43568 -COMMA- denotes ,
T21849 43569-43572 CC denotes and
T21850 43573-43576 DT denotes the
T21851 43577-43586 NN denotes membranes
T21852 43587-43591 VB denotes were
T21853 43592-43604 RB denotes subsequently
T21854 43605-43614 VB denotes incubated
T21855 43615-43619 IN denotes with
T21856 43620-43622 DT denotes an
T21857 43623-43624 CD denotes 1
T21858 43624-43625 -COLON- denotes :
T21859 43625-43628 CD denotes 200
T21860 43629-43637 NN denotes dilution
T21861 43638-43640 IN denotes of
T21862 43641-43645 NN denotes goat
T21863 43646-43656 NN denotes anti-FOXP3
T21864 43657-43659 IN denotes in
T21865 43660-43668 VB denotes blocking
T21866 43669-43675 NN denotes buffer
T21867 43676-43677 -LRB- denotes (
T21868 43677-43682 NN denotes Abcam
T21869 43682-43683 -RRB- denotes )
T21870 43684-43693 RB denotes overnight
T21871 43694-43696 IN denotes at
T21872 43697-43698 CD denotes 4
T21873 43699-43702 NNP denotes °C.
T21874 43703-43706 DT denotes The
T21875 43707-43712 NN denotes blots
T21876 43713-43717 VB denotes were
T21877 43718-43727 VB denotes developed
T21878 43728-43733 VB denotes using
T21879 43734-43736 DT denotes an
T21880 43737-43746 JJ denotes anti-goat
T21881 43747-43758 JJ denotes HRP-labeled
T21882 43759-43762 NN denotes mAb
T21883 43763-43764 -LRB- denotes (
T21884 43764-43772 NNP denotes Amersham
T21885 43773-43784 NNP denotes Biosciences
T21886 43784-43785 -RRB- denotes )
T21887 43786-43789 CC denotes and
T21888 43790-43800 VB denotes visualized
T21889 43801-43805 IN denotes with
T21890 43806-43807 DT denotes a
T21891 43808-43811 NN denotes LAS
T21892 43812-43816 CD denotes 1000
T21893 43817-43823 NN denotes camera
T21894 43824-43825 -LRB- denotes (
T21895 43825-43829 NN denotes Fuji
T21896 43829-43830 -RRB- denotes )
T21897 43832-43841 NN denotes Membranes
T21898 43842-43846 VB denotes were
T21899 43847-43856 VB denotes incubated
T21900 43857-43859 IN denotes in
T21901 43860-43869 VB denotes stripping
T21902 43870-43876 NN denotes buffer
T21903 43877-43880 CC denotes and
T21904 43881-43891 JJ denotes re-blocked
T21905 43892-43895 IN denotes for
T21906 43896-43897 CD denotes 1
T21907 43898-43899 NN denotes h
T21908 43901-43904 DT denotes The
T21909 43905-43914 NN denotes membranes
T21910 43915-43919 VB denotes were
T21911 43920-43929 VB denotes re-probed
T21912 43930-43935 VB denotes using
T21913 43936-43946 NN denotes anti-GATA3
T21914 43947-43948 -LRB- denotes (
T21915 43948-43954 NN denotes HG3–31
T21916 43954-43955 -COLON- denotes ;
T21917 43956-43961 NNP denotes Santa
T21918 43962-43966 NNP denotes Cruz
T21919 43967-43980 NNP denotes Biotechnology
T21920 43980-43981 -RRB- denotes )
T21921 43981-43982 -COMMA- denotes ,
T21922 43983-43993 JJ denotes anti-T-bet
T21923 43994-43995 -LRB- denotes (
T21924 43995-43999 NN denotes 4B10
T21925 43999-44000 -COMMA- denotes ,
T21926 44001-44006 NNP denotes Santa
T21927 44007-44011 NNP denotes Cruz
T21928 44012-44025 NNP denotes Biotechnology
T21929 44025-44026 -RRB- denotes )
T21930 44026-44027 -COMMA- denotes ,
T21931 44028-44038 NN denotes anti-GAPDH
T21932 44039-44040 -LRB- denotes (
T21933 44040-44043 NN denotes 6C5
T21934 44043-44044 -COMMA- denotes ,
T21935 44045-44051 NNP denotes Ambion
T21936 44051-44052 -RRB- denotes )
T21937 44052-44053 -COMMA- denotes ,
T21938 44054-44072 NN denotes anti-phospho-SMAD2
T21939 44073-44074 -LRB- denotes (
T21940 44074-44079 NN denotes 138D4
T21941 44079-44080 -RRB- denotes )
T21942 44080-44081 -COMMA- denotes ,
T21943 44082-44100 NN denotes anti-phospho-STAT6
T21944 44101-44102 -LRB- denotes (
T21945 44102-44105 NN denotes 5A4
T21946 44105-44106 -RRB- denotes )
T21947 44106-44107 -COMMA- denotes ,
T21948 44108-44111 CC denotes and
T21949 44112-44122 JJ denotes anti-STAT6
T21950 44123-44124 -LRB- denotes (
T21951 44124-44128 NNP denotes Cell
T21952 44129-44138 NNP denotes Signaling
T21953 44139-44149 NNP denotes Technology
T21954 44149-44150 -RRB- denotes )
T21955 44150-44151 -COMMA- denotes ,
T22260 44153-44167 NN denotes Administration
T22261 44168-44170 IN denotes of
T22262 44171-44180 NN denotes cytokines
T22263 44181-44184 CC denotes and
T22264 44185-44195 NN denotes antibodies
T22265 44196-44198 FW denotes in
T22266 44199-44203 FW denotes vivo
T22267 44205-44209 NN denotes Age-
T22268 44210-44213 CC denotes and
T22269 44214-44228 JJ denotes gender-matched
T22270 44229-44235 JJ denotes normal
T22271 44236-44238 NN denotes B6
T22272 44239-44243 NN denotes mice
T22273 44244-44252 VB denotes received
T22274 44253-44258 DT denotes every
T22275 44259-44264 JJ denotes other
T22276 44265-44268 NN denotes day
T22277 44269-44284 JJ denotes intraperitoneal
T22278 44285-44286 -LRB- denotes (
T22279 44286-44288 JJ denotes ip
T22280 44288-44289 -RRB- denotes )
T22281 44290-44300 NN denotes injections
T22282 44301-44303 IN denotes of
T22283 44304-44307 NNP denotes PBS
T22284 44307-44308 -COMMA- denotes ,
T22285 44309-44312 CD denotes 1.5
T22286 44313-44315 NN denotes μg
T22287 44316-44322 NN denotes rmIL-4
T22288 44322-44323 -COMMA- denotes ,
T22289 44324-44326 CD denotes 50
T22290 44327-44329 CC denotes μg
T22291 44330-44339 JJ denotes anti-IL-4
T22292 44340-44343 NN denotes mAb
T22293 44344-44345 -LRB- denotes (
T22294 44345-44350 NN denotes 11B11
T22295 44351-44353 CC denotes or
T22296 44354-44360 NN denotes MAB404
T22297 44360-44361 -RRB- denotes )
T22298 44361-44362 -COMMA- denotes ,
T22299 44363-44365 CC denotes or
T22300 44366-44367 DT denotes a
T22301 44368-44375 NN denotes mixture
T22302 44376-44378 IN denotes of
T22303 44379-44382 CD denotes 1.5
T22304 44383-44385 NN denotes μg
T22305 44386-44392 NN denotes rmIL-4
T22306 44393-44397 CC denotes plus
T22307 44398-44400 CD denotes 50
T22308 44401-44403 CC denotes μg
T22309 44404-44413 JJ denotes anti-IL-4
T22310 44414-44417 NN denotes mAb
T22311 44418-44419 -LRB- denotes (
T22312 44419-44424 NN denotes 11B11
T22313 44425-44427 CC denotes or
T22314 44428-44434 NN denotes MAB404
T22315 44434-44435 -RRB- denotes )
T22316 44436-44439 IN denotes for
T22317 44440-44441 CD denotes 7
T22318 44442-44443 NN denotes d
T22319 44445-44455 RB denotes Thereafter
T22320 44455-44456 -COMMA- denotes ,
T22321 44457-44463 NN denotes spleen
T22322 44464-44467 CC denotes and
T22323 44468-44473 NN denotes lymph
T22324 44474-44478 NN denotes node
T22325 44479-44484 NN denotes cells
T22326 44485-44489 VB denotes were
T22327 44490-44498 VB denotes analyzed
T22328 44499-44501 IN denotes by
T22329 44502-44506 NN denotes flow
T22330 44507-44516 NN denotes cytometry
T22331 44517-44520 IN denotes for
T22332 44521-44524 NN denotes CD3
T22333 44524-44525 -COMMA- denotes ,
T22334 44526-44529 NN denotes CD4
T22335 44529-44530 -COMMA- denotes ,
T22336 44531-44534 CC denotes and
T22337 44535-44539 NN denotes CD25
T22338 44540-44550 NN denotes expression
T22339 44552-44555 DT denotes The
T22340 44556-44566 JJ denotes anti-mouse
T22341 44567-44571 NN denotes IL-4
T22342 44572-44575 NN denotes mAb
T22343 44576-44582 NN denotes MAB404
T22344 44583-44586 VB denotes was
T22345 44587-44595 VB denotes obtained
T22346 44596-44600 IN denotes from
T22347 44601-44602 NNP denotes R
T22348 44602-44603 CC denotes &
T22349 44603-44604 NNP denotes D
T22350 44605-44612 NNP denotes Systems
T22351 44612-44613 -COMMA- denotes ,
T22352 44614-44617 DT denotes the
T22353 44618-44624 JJ denotes second
T22354 44625-44635 JJ denotes anti-mouse
T22355 44636-44640 NN denotes IL-4
T22356 44641-44644 NN denotes mAb
T22357 44645-44650 NN denotes 11B11
T22358 44651-44654 VB denotes was
T22359 44655-44664 VB denotes purchased
T22360 44665-44669 IN denotes from
T22361 44670-44681 NN denotes eBioscience
T22574 44684-44688 NN denotes ChIP
T22575 44690-44694 NN denotes ChIP
T22576 44695-44703 NN denotes analysis
T22577 44704-44707 VB denotes was
T22578 44708-44717 VB denotes performed
T22579 44718-44727 VB denotes according
T22580 44728-44730 TO denotes to
T22581 44731-44734 DT denotes the
T22582 44735-44747 NN denotes manufacturer
T22583 44747-44749 POS denotes 's
T22584 44750-44758 NN denotes protocol
T22585 44759-44760 -LRB- denotes (
T22586 44760-44767 NNP denotes Upstate
T22587 44768-44781 NNP denotes Biotechnology
T22588 44781-44782 -RRB- denotes )
T22589 44783-44787 IN denotes with
T22590 44788-44791 DT denotes the
T22591 44792-44801 VB denotes following
T22592 44802-44816 NN denotes modifications.
T22593 44817-44822 NN denotes iTreg
T22594 44823-44826 CC denotes and
T22595 44827-44830 NN denotes Th2
T22596 44831-44836 NN denotes cells
T22597 44837-44841 VB denotes were
T22598 44842-44847 VB denotes fixed
T22599 44848-44852 IN denotes with
T22600 44853-44854 CD denotes 1
T22601 44854-44855 NN denotes %
T22602 44856-44868 NN denotes formaldehyde
T22603 44869-44872 IN denotes for
T22604 44873-44875 CD denotes 10
T22605 44876-44879 NN denotes min
T22606 44880-44882 IN denotes at
T22607 44883-44887 NN denotes room
T22608 44888-44899 NN denotes temperature
T22609 44901-44904 DT denotes The
T22610 44905-44914 NN denotes chromatin
T22611 44915-44918 VB denotes was
T22612 44919-44926 VB denotes sheared
T22613 44927-44929 TO denotes to
T22614 44930-44938 CD denotes 200-1000
T22615 44939-44941 NN denotes bp
T22616 44942-44944 IN denotes of
T22617 44945-44951 NN denotes length
T22618 44952-44954 IN denotes by
T22619 44955-44965 NN denotes sonication
T22620 44966-44970 IN denotes with
T22621 44971-44975 CD denotes five
T22622 44976-44982 NN denotes pulses
T22623 44983-44985 IN denotes of
T22624 44986-44988 CD denotes 10
T22625 44989-44990 NN denotes s
T22626 44991-44993 IN denotes at
T22627 44994-44996 CD denotes 30
T22628 44996-44997 NN denotes %
T22629 44998-45003 NN denotes power
T22630 45004-45005 -LRB- denotes (
T22631 45005-45013 NN denotes Bandelin
T22632 45013-45014 -RRB- denotes )
T22633 45016-45019 DT denotes The
T22634 45020-45029 NN denotes chromatin
T22635 45030-45033 VB denotes was
T22636 45034-45045 JJ denotes pre-cleared
T22637 45046-45049 IN denotes for
T22638 45050-45051 CD denotes 2
T22639 45052-45053 NN denotes h
T22640 45054-45058 IN denotes with
T22641 45059-45065 JJ denotes normal
T22642 45066-45071 NN denotes mouse
T22643 45072-45075 NN denotes IgG
T22644 45076-45081 NN denotes beads
T22645 45082-45085 CC denotes and
T22646 45086-45090 RB denotes then
T22647 45091-45100 VB denotes incubated
T22648 45101-45105 IN denotes with
T22649 45106-45124 JJ denotes anti-GATA3-agarose
T22650 45125-45130 NN denotes beads
T22651 45131-45132 -LRB- denotes (
T22652 45132-45138 NN denotes HG3–31
T22653 45138-45139 -COLON- denotes ;
T22654 45140-45145 NNP denotes Santa
T22655 45146-45150 NNP denotes Cruz
T22656 45151-45164 NNP denotes Biotechnology
T22657 45164-45165 -RRB- denotes )
T22658 45166-45169 IN denotes for
T22659 45170-45171 CD denotes 2
T22660 45172-45173 NN denotes h
T22661 45175-45182 NN denotes Washing
T22662 45183-45186 CC denotes and
T22663 45187-45194 NN denotes elution
T22664 45195-45202 NN denotes buffers
T22665 45203-45207 VB denotes were
T22666 45208-45212 VB denotes used
T22667 45213-45222 VB denotes according
T22668 45223-45225 TO denotes to
T22669 45226-45229 DT denotes the
T22670 45230-45238 NN denotes protocol
T22671 45239-45241 IN denotes of
T22672 45242-45249 NNP denotes Upstate
T22673 45250-45264 NNP denotes Biotechnology.
T22674 45265-45275 NNP denotes Crosslinks
T22675 45276-45280 VB denotes were
T22676 45281-45289 VB denotes reversed
T22677 45290-45292 IN denotes by
T22678 45293-45303 NN denotes incubation
T22679 45304-45306 IN denotes at
T22680 45307-45309 CD denotes 65
T22681 45310-45312 NN denotes °C
T22682 45313-45316 IN denotes for
T22683 45317-45318 CD denotes 4
T22684 45319-45320 NN denotes h
T22685 45321-45323 IN denotes in
T22686 45324-45327 DT denotes the
T22687 45328-45336 NN denotes presence
T22688 45337-45339 IN denotes of
T22689 45340-45343 CD denotes 0.2
T22690 45344-45345 NN denotes M
T22691 45346-45350 NN denotes NaCl
T22692 45350-45351 -COMMA- denotes ,
T22693 45352-45355 CC denotes and
T22694 45356-45359 DT denotes the
T22695 45360-45363 NN denotes DNA
T22696 45364-45367 VB denotes was
T22697 45368-45376 VB denotes purified
T22698 45377-45379 IN denotes by
T22699 45380-45397 NN denotes phenol/chloroform
T22700 45398-45408 NN denotes extraction
T22701 45410-45413 DT denotes The
T22702 45414-45420 NN denotes amount
T22703 45421-45423 IN denotes of
T22704 45424-45427 NN denotes DNA
T22705 45428-45431 VB denotes was
T22706 45432-45442 VB denotes determined
T22707 45443-45445 IN denotes by
T22708 45446-45458 JJ denotes conventional
T22709 45459-45462 NN denotes PCR
T22710 45464-45467 DT denotes The
T22711 45468-45471 NN denotes PCR
T22712 45472-45481 VB denotes addressed
T22713 45482-45485 IN denotes for
T22714 45486-45489 DT denotes the
T22715 45490-45495 NN denotes FOXP3
T22716 45496-45504 NN denotes promoter
T22717 45505-45511 NN denotes region
T22718 45512-45516 CD denotes −246
T22719 45517-45519 TO denotes to
T22720 45520-45524 CD denotes −511
T22721 45525-45528 CC denotes and
T22722 45529-45532 VB denotes was
T22723 45533-45542 VB denotes performed
T22724 45543-45548 VB denotes using
T22725 45549-45552 DT denotes the
T22726 45553-45562 VB denotes following
T22727 45563-45570 NN denotes primers
T22728 45570-45571 -COLON- denotes :
T22729 45572-45590 JJ denotes 5′-gtgccctttacgagt
T22730 45591-45600 NN denotes catctg-3′
T22731 45601-45604 CC denotes and
T22732 45605-45632 NN denotes 5′-gtgccctttacgagtcatctg-3′
T22733 45634-45637 DT denotes The
T22734 45638-45641 NN denotes PCR
T22735 45642-45650 NN denotes products
T22736 45651-45655 VB denotes were
T22737 45656-45666 VB denotes visualized
T22738 45667-45672 VB denotes using
T22739 45673-45675 DT denotes an
T22740 45676-45684 NN denotes ethidium
T22741 45685-45692 NN denotes bromide
T22742 45693-45696 NN denotes gel
T22975 45699-45708 JJ denotes Pull-down
T22976 45709-45714 NN denotes assay
T22977 45716-45720 JJ denotes CD4+
T22978 45721-45722 NN denotes T
T22979 45723-45728 NN denotes cells
T22980 45729-45733 VB denotes were
T22981 45734-45744 VB denotes stimulated
T22982 45745-45749 IN denotes with
T22983 45750-45753 NN denotes PMA
T22984 45754-45757 CC denotes and
T22985 45758-45767 NN denotes ionomycin
T22986 45768-45771 IN denotes for
T22987 45772-45773 CD denotes 2
T22988 45774-45775 NN denotes h
T22989 45776-45778 IN denotes at
T22990 45779-45784 NNP denotes 37°C.
T22991 45785-45788 DT denotes The
T22992 45789-45794 NN denotes cells
T22993 45795-45799 VB denotes were
T22994 45800-45808 VB denotes pelleted
T22995 45808-45809 -COMMA- denotes ,
T22996 45810-45821 VB denotes resuspended
T22997 45822-45824 IN denotes in
T22998 45825-45831 NN denotes buffer
T22999 45832-45833 NN denotes C
T23000 45834-45835 -LRB- denotes (
T23001 45835-45837 CD denotes 20
T23002 45838-45840 NN denotes mM
T23003 45841-45846 NN denotes HEPES
T23004 45847-45848 -LRB- denotes [
T23005 45848-45850 NN denotes pH
T23006 45851-45854 CD denotes 7.9
T23007 45854-45855 -RRB- denotes ]
T23008 45855-45856 -COMMA- denotes ,
T23009 45857-45860 CD denotes 420
T23010 45861-45863 NN denotes mM
T23011 45864-45868 NN denotes NaCl
T23012 45868-45869 -COMMA- denotes ,
T23013 45870-45873 CD denotes 1.5
T23014 45874-45876 NN denotes mM
T23015 45877-45882 NN denotes MgCl2
T23016 45882-45883 -COMMA- denotes ,
T23017 45884-45887 CD denotes 0.2
T23018 45888-45890 NN denotes mM
T23019 45891-45895 NN denotes EDTA
T23020 45895-45896 -COMMA- denotes ,
T23021 45897-45898 CD denotes 1
T23022 45899-45901 NN denotes mM
T23023 45902-45905 NN denotes DTT
T23024 45905-45906 -COMMA- denotes ,
T23025 45907-45915 NN denotes protease
T23026 45916-45926 NN denotes inhibitors
T23027 45927-45928 -LRB- denotes [
T23028 45928-45933 NNP denotes Sigma
T23029 45933-45934 -RRB- denotes ]
T23030 45934-45937 CC denotes . a
T23031 45937-45940 CD denotes nd
T23032 45940-45941 NN denotes 0
T23033 45941-45946 NN denotes .1% N
T23034 45946-45947 -RRB- denotes P
T23035 45947-45950 CC denotes -40
T23036 45950-45955 VB denotes ) and
T23037 45956-45958 IN denotes ly
T23038 45958-45961 NN denotes sed
T23039 45962-45965 IN denotes on
T23040 45965-45967 CD denotes ic
T23041 45967-45970 NN denotes e f
T23042 45981-45990 JJ denotes Insoluble
T23043 45991-45999 NN denotes material
T23044 46000-46003 VB denotes was
T23045 46004-46011 VB denotes removed
T23046 46012-46014 IN denotes by
T23047 46015-46029 NN denotes centrifugation
T23048 46031-46034 DT denotes The
T23049 46035-46046 NN denotes supernatant
T23050 46047-46050 VB denotes was
T23051 46051-46058 VB denotes diluted
T23052 46059-46060 CD denotes 1
T23053 46060-46061 -COLON- denotes :
T23054 46061-46062 CD denotes 3
T23055 46063-46067 IN denotes with
T23056 46068-46074 NN denotes buffer
T23057 46075-46076 NN denotes D
T23058 46077-46078 -LRB- denotes (
T23059 46078-46080 IN denotes as
T23060 46081-46087 NN denotes buffer
T23061 46088-46089 NN denotes C
T23062 46089-46090 -COMMA- denotes ,
T23063 46091-46094 CC denotes but
T23064 46095-46102 IN denotes without
T23065 46103-46107 NN denotes NaCl
T23066 46107-46108 -RRB- denotes )
T23067 46110-46113 DT denotes The
T23068 46114-46121 NN denotes lysates
T23069 46122-46126 VB denotes were
T23070 46127-46136 VB denotes incubated
T23071 46137-46141 IN denotes with
T23072 46142-46144 CD denotes 10
T23073 46145-46147 NN denotes μg
T23074 46148-46150 IN denotes of
T23075 46151-46155 NN denotes poly
T23076 46155-46156 -LRB- denotes (
T23077 46156-46161 NN denotes dI-dC
T23078 46161-46162 -RRB- denotes )
T23079 46163-46164 -LRB- denotes (
T23080 46164-46169 NN denotes Sigma
T23081 46169-46170 -RRB- denotes )
T23082 46171-46174 CC denotes and
T23083 46175-46177 CD denotes 70
T23084 46178-46180 NN denotes μl
T23085 46181-46183 IN denotes of
T23086 46184-46204 JJ denotes streptavidin-agarose
T23087 46205-46206 -LRB- denotes (
T23088 46206-46214 NNP denotes Amersham
T23089 46215-46226 NNP denotes Biosciences
T23090 46226-46227 -RRB- denotes )
T23091 46228-46236 VB denotes carrying
T23092 46237-46249 VB denotes biotinylated
T23093 46250-46266 NN denotes oligonucleotides
T23094 46266-46267 -COMMA- denotes ,
T23095 46268-46271 IN denotes for
T23096 46272-46273 CD denotes 3
T23097 46274-46275 NN denotes h
T23098 46276-46278 IN denotes at
T23099 46279-46280 CD denotes 4
T23100 46281-46284 NNP denotes °C.
T23101 46285-46288 DT denotes The
T23102 46289-46294 NN denotes beads
T23103 46295-46299 VB denotes were
T23104 46300-46306 VB denotes washed
T23105 46307-46312 RB denotes twice
T23106 46313-46317 IN denotes with
T23107 46318-46324 NN denotes buffer
T23108 46325-46326 NN denotes C
T23109 46326-46327 -COLON- denotes :
T23110 46327-46328 NN denotes D
T23111 46329-46330 -LRB- denotes (
T23112 46330-46331 CD denotes 1
T23113 46331-46332 -COLON- denotes :
T23114 46332-46333 CD denotes 3
T23115 46333-46334 -RRB- denotes )
T23116 46335-46338 CC denotes and
T23117 46339-46350 VB denotes resuspended
T23118 46351-46353 IN denotes in
T23119 46354-46368 JJ denotes DTT-containing
T23120 46369-46376 NN denotes loading
T23121 46377-46383 NN denotes buffer
T23122 46384-46385 -LRB- denotes (
T23123 46385-46391 NN denotes NuPAGE
T23124 46391-46392 -COLON- denotes ;
T23125 46393-46403 NN denotes Invitrogen
T23126 46403-46404 -RRB- denotes )
T23127 46404-46405 -COMMA- denotes ,
T23128 46406-46412 VB denotes heated
T23129 46413-46415 TO denotes to
T23130 46416-46418 CD denotes 70
T23131 46419-46421 NN denotes °C
T23132 46422-46425 IN denotes for
T23133 46426-46428 CD denotes 10
T23134 46429-46432 NN denotes min
T23135 46432-46433 -COMMA- denotes ,
T23136 46434-46437 CC denotes and
T23137 46438-46441 DT denotes the
T23138 46442-46449 NN denotes eluants
T23139 46450-46452 IN denotes on
T23140 46453-46454 DT denotes a
T23141 46455-46461 NN denotes NuPAGE
T23142 46462-46466 CD denotes 4–12
T23143 46466-46467 NN denotes %
T23144 46468-46476 JJ denotes bis-tris
T23145 46477-46480 NN denotes gel
T23146 46481-46482 -LRB- denotes (
T23147 46482-46492 NN denotes Invitrogen
T23148 46492-46493 -RRB- denotes )
T23149 46495-46498 DT denotes The
T23150 46499-46507 NN denotes proteins
T23151 46508-46512 VB denotes were
T23152 46513-46527 VB denotes electroblotted
T23153 46528-46532 IN denotes onto
T23154 46533-46534 DT denotes a
T23155 46535-46539 NN denotes PVDF
T23156 46540-46548 NN denotes membrane
T23157 46549-46550 -LRB- denotes (
T23158 46550-46558 NNP denotes Amersham
T23159 46559-46570 NNP denotes Biosciences
T23160 46570-46571 -RRB- denotes )
T23161 46572-46575 CC denotes and
T23162 46576-46584 VB denotes detected
T23163 46585-46590 VB denotes using
T23164 46591-46593 DT denotes an
T23165 46594-46604 JJ denotes anti-GATA3
T23166 46605-46608 NN denotes mAb
T23167 46609-46610 -LRB- denotes (
T23168 46610-46615 NNP denotes Santa
T23169 46616-46620 NNP denotes Cruz
T23170 46621-46634 NNP denotes Biotechnology
T23171 46634-46635 -RRB- denotes )
T23172 46637-46648 VB denotes Accumulated
T23173 46649-46656 NN denotes signals
T23174 46657-46661 VB denotes were
T23175 46662-46670 VB denotes analyzed
T23176 46671-46676 VB denotes using
T23177 46677-46681 NN denotes AIDA
T23178 46682-46690 NN denotes software
T23179 46691-46692 -LRB- denotes (
T23180 46692-46699 NN denotes Raytest
T23181 46699-46700 -RRB- denotes )
T23464 7387-7390 NN denotes Th2
T23465 7391-7396 NN denotes Cells
T23466 7397-7400 MD denotes Can
T23467 7400-7403 RB denotes not
T23468 7404-7410 VB denotes Induce
T23469 7411-7416 NN denotes FOXP3
T23470 7417-7427 NN denotes Expression
T23471 7428-7429 -LRB- denotes (
T23472 7429-7430 NN denotes A
T23473 7430-7431 -RRB- denotes )
T23474 7432-7437 JJ denotes Human
T23475 7438-7439 NN denotes T
T23476 7440-7445 NN denotes cells
T23477 7446-7450 VB denotes were
T23478 7451-7460 VB denotes activated
T23479 7461-7465 IN denotes with
T23480 7466-7477 JJ denotes plate-bound
T23481 7478-7491 NN denotes anti-CD3/CD28
T23482 7492-7496 IN denotes with
T23483 7497-7499 CC denotes or
T23484 7500-7507 IN denotes without
T23485 7508-7513 NN denotes TGF-β
T23486 7514-7516 IN denotes as
T23487 7517-7526 VB denotes indicated
T23488 7527-7529 IN denotes on
T23489 7530-7533 DT denotes the
T23490 7534-7540 NN denotes x-axis
T23491 7541-7543 IN denotes of
T23492 7544-7545 -LRB- denotes (
T23493 7545-7546 NN denotes B
T23494 7546-7547 -RRB- denotes )
T23495 7549-7554 NN denotes Cells
T23496 7555-7559 VB denotes were
T23497 7560-7569 VB denotes harvested
T23498 7570-7575 IN denotes after
T23499 7576-7577 CD denotes 5
T23500 7578-7582 NN denotes days
T23501 7583-7586 CC denotes and
T23502 7587-7592 NN denotes FOXP3
T23503 7593-7597 NN denotes mRNA
T23504 7598-7601 VB denotes was
T23505 7602-7612 VB denotes quantified
T23506 7613-7615 IN denotes by
T23507 7616-7625 JJ denotes real-time
T23508 7626-7629 NN denotes PCR
T23509 7631-7635 NN denotes Bars
T23510 7636-7640 VB denotes show
T23511 7641-7644 DT denotes the
T23512 7645-7649 JJ denotes mean
T23513 7650-7651 JJ denotes ±
T23514 7652-7654 NN denotes SD
T23515 7655-7657 IN denotes of
T23516 7658-7659 CD denotes 4
T23517 7660-7671 JJ denotes independent
T23518 7672-7683 NN denotes experiments
T23519 7685-7686 -LRB- denotes (
T23520 7686-7687 NN denotes B
T23521 7687-7688 -RRB- denotes )
T23522 7689-7691 FW denotes In
T23523 7692-7697 FW denotes vitro
T23524 7698-7712 VB denotes differentiated
T23525 7713-7716 NN denotes Th1
T23526 7716-7717 -COMMA- denotes ,
T23527 7718-7721 NN denotes Th2
T23528 7721-7722 -COMMA- denotes ,
T23529 7723-7725 CC denotes or
T23530 7726-7731 NN denotes iTreg
T23531 7732-7737 NN denotes cells
T23532 7738-7742 VB denotes were
T23533 7743-7752 VB denotes activated
T23534 7753-7757 IN denotes with
T23535 7758-7771 JJ denotes anti-CD3/CD28
T23536 7771-7772 -COMMA- denotes ,
T23537 7773-7778 NN denotes TGF-β
T23538 7778-7779 -COMMA- denotes ,
T23539 7780-7782 CC denotes or
T23540 7783-7792 NN denotes anti-IL-4
T23541 7793-7795 IN denotes as
T23542 7796-7805 VB denotes indicated
T23543 7807-7810 DT denotes The
T23544 7811-7820 NN denotes phenotype
T23545 7821-7823 IN denotes of
T23546 7824-7829 DT denotes these
T23547 7830-7835 NN denotes cells
T23548 7836-7839 VB denotes was
T23549 7840-7849 VB denotes confirmed
T23550 7850-7852 IN denotes by
T23551 7853-7857 NN denotes FACS
T23552 7858-7861 CC denotes and
T23553 7862-7875 NN denotes proliferation
T23554 7876-7884 NN denotes analysis
T23555 7885-7886 -LRB- denotes (
T23556 7886-7892 NN denotes Figure
T23557 7893-7895 NN denotes S1
T23558 7895-7896 -RRB- denotes )
T23559 7898-7902 NN denotes Bars
T23560 7903-7907 VB denotes show
T23561 7908-7911 DT denotes the
T23562 7912-7916 JJ denotes mean
T23563 7917-7918 JJ denotes ±
T23564 7919-7921 NN denotes SD
T23565 7922-7924 IN denotes of
T23566 7925-7929 CD denotes four
T23567 7930-7941 JJ denotes independent
T23568 7942-7953 NN denotes experiments
T23815 10140-10143 NN denotes Th2
T23816 10144-10149 NN denotes Cells
T23817 10150-10152 VB denotes Do
T23818 10153-10156 NNP denotes Not
T23819 10157-10164 NNP denotes Express
T23820 10165-10170 NN denotes FOXP3
T23821 10171-10172 -LRB- denotes (
T23822 10172-10173 NN denotes A
T23823 10173-10174 -RRB- denotes )
T23824 10175-10188 JJ denotes Intracellular
T23825 10189-10193 NN denotes FACS
T23826 10194-10202 NN denotes analysis
T23827 10203-10205 IN denotes of
T23828 10206-10211 NN denotes FOXP3
T23829 10212-10222 NN denotes expression
T23830 10223-10225 IN denotes in
T23831 10226-10229 NN denotes Th1
T23832 10229-10230 -COMMA- denotes ,
T23833 10231-10234 NN denotes Th2
T23834 10234-10235 -COMMA- denotes ,
T23835 10236-10238 CC denotes or
T23836 10239-10259 JJ denotes iTreg-differentiated
T23837 10260-10265 NN denotes cells
T23838 10266-10267 -LRB- denotes (
T23839 10267-10270 CD denotes two
T23840 10271-10277 NN denotes rounds
T23841 10277-10278 -COMMA- denotes ,
T23842 10279-10288 NN denotes phenotype
T23843 10289-10292 VB denotes see
T23844 10293-10299 NN denotes Figure
T23845 10300-10302 NN denotes S1
T23846 10302-10303 -RRB- denotes )
T23847 10303-10304 -COMMA- denotes ,
T23848 10305-10311 VB denotes rested
T23849 10312-10314 CC denotes or
T23850 10315-10324 VB denotes activated
T23851 10324-10325 -COMMA- denotes ,
T23852 10326-10330 IN denotes with
T23853 10331-10333 CC denotes or
T23854 10334-10341 IN denotes without
T23855 10342-10347 NN denotes TGF-β
T23856 10349-10354 NN denotes FOXP3
T23857 10355-10365 NN denotes expression
T23858 10366-10369 VB denotes was
T23859 10370-10378 VB denotes measured
T23860 10379-10384 IN denotes after
T23861 10385-10386 CD denotes 5
T23862 10387-10388 NN denotes d
T23863 10389-10391 IN denotes in
T23864 10392-10399 NN denotes culture
T23865 10401-10404 DT denotes The
T23866 10405-10408 NN denotes dot
T23867 10409-10414 NN denotes blots
T23868 10415-10418 VB denotes are
T23869 10419-10433 JJ denotes representative
T23870 10434-10436 IN denotes of
T23871 10437-10442 CD denotes three
T23872 10443-10454 JJ denotes independent
T23873 10455-10466 NN denotes experiments
T23874 10468-10469 -LRB- denotes (
T23875 10469-10470 NN denotes B
T23876 10470-10471 -RRB- denotes )
T23877 10472-10477 VB denotes Shows
T23878 10478-10481 DT denotes the
T23879 10482-10486 JJ denotes same
T23880 10487-10499 JJ denotes experimental
T23881 10500-10505 NN denotes setup
T23882 10505-10506 -COMMA- denotes ,
T23883 10507-10510 CC denotes but
T23884 10511-10520 RB denotes naturally
T23885 10521-10530 VB denotes occurring
T23886 10531-10534 NN denotes Th2
T23887 10535-10540 NN denotes cells
T23888 10541-10545 VB denotes were
T23889 10546-10554 VB denotes analyzed
T23890 10556-10560 NN denotes Data
T23891 10561-10564 VB denotes are
T23892 10565-10579 JJ denotes representative
T23893 10580-10582 IN denotes of
T23894 10583-10588 CD denotes three
T23895 10589-10600 JJ denotes independent
T23896 10601-10612 NN denotes experiments
T24133 10624-10628 NN denotes Th2-
T24134 10629-10631 CC denotes or
T24135 10632-10646 JJ denotes IL-4–Producing
T24136 10647-10652 NNP denotes Cells
T24137 10653-10657 NN denotes Lack
T24138 10658-10663 NN denotes FOXP3
T24139 10664-10665 -LRB- denotes (
T24140 10665-10666 NN denotes A
T24141 10666-10667 -RRB- denotes )
T24142 10668-10672 NN denotes FACS
T24143 10673-10681 NN denotes analysis
T24144 10682-10684 IN denotes of
T24145 10685-10698 JJ denotes intracellular
T24146 10699-10704 NN denotes FOXP3
T24147 10705-10708 CC denotes and
T24148 10709-10713 NN denotes IL-4
T24149 10714-10724 NN denotes expression
T24150 10725-10734 VB denotes following
T24151 10735-10748 NN denotes PMA/Ionomycin
T24152 10749-10760 NN denotes stimulation
T24153 10762-10766 JJ denotes CD4+
T24154 10767-10768 NN denotes T
T24155 10769-10774 NN denotes cells
T24156 10775-10779 VB denotes were
T24157 10780-10785 VB denotes gated
T24158 10786-10788 IN denotes on
T24159 10789-10792 DT denotes the
T24160 10793-10798 NN denotes basis
T24161 10799-10801 IN denotes of
T24162 10802-10808 NN denotes CD45RO
T24163 10809-10812 CC denotes and
T24164 10813-10817 NN denotes CD25
T24165 10818-10825 NN denotes surface
T24166 10826-10836 NN denotes expression
T24167 10837-10838 -LRB- denotes (
T24168 10838-10843 JJ denotes upper
T24169 10844-10849 NN denotes panel
T24170 10849-10850 -RRB- denotes )
T24171 10850-10851 -COMMA- denotes ,
T24172 10852-10855 CC denotes and
T24173 10856-10861 VB denotes gated
T24174 10862-10867 NN denotes cells
T24175 10868-10871 VB denotes are
T24176 10872-10877 VB denotes shown
T24177 10878-10883 IN denotes below
T24178 10884-10887 IN denotes for
T24179 10888-10891 DT denotes the
T24180 10892-10904 NN denotes CD45RO+CD25−
T24181 10905-10906 -LRB- denotes (
T24182 10906-10907 NN denotes A
T24183 10907-10908 -COMMA- denotes ,
T24184 10909-10913 JJ denotes left
T24185 10914-10919 NN denotes panel
T24186 10919-10920 -RRB- denotes )
T24187 10920-10921 -COMMA- denotes ,
T24188 10922-10925 DT denotes the
T24189 10926-10938 JJ denotes CD45RO+CD25+
T24190 10939-10940 -LRB- denotes (
T24191 10940-10945 JJ denotes right
T24192 10946-10951 NN denotes panel
T24193 10951-10952 -RRB- denotes )
T24194 10952-10953 -COMMA- denotes ,
T24195 10954-10957 CC denotes and
T24196 10958-10961 DT denotes the
T24197 10962-10974 NN denotes CD45RO−CD25−
T24198 10975-10982 NN denotes subsets
T24199 10983-10984 -LRB- denotes (
T24200 10984-10991 JJ denotes central
T24201 10992-10997 NN denotes panel
T24202 10997-10998 -RRB- denotes )
T24203 11000-11001 DT denotes A
T24204 11002-11013 JJ denotes statistical
T24205 11014-11022 NN denotes analysis
T24206 11023-11025 IN denotes of
T24207 11026-11031 CD denotes eight
T24208 11032-11043 JJ denotes independent
T24209 11044-11050 NN denotes donors
T24210 11051-11056 IN denotes after
T24211 11057-11068 NN denotes subtraction
T24212 11069-11071 IN denotes of
T24213 11072-11075 DT denotes the
T24214 11076-11083 NN denotes isotype
T24215 11084-11091 NN denotes control
T24216 11092-11095 VB denotes are
T24217 11096-11101 VB denotes shown
T24218 11102-11104 IN denotes in
T24219 11105-11106 -LRB- denotes (
T24220 11106-11107 NN denotes B
T24221 11107-11108 -RRB- denotes )
T24222 11110-11113 DT denotes The
T24223 11114-11120 VB denotes dotted
T24224 11121-11125 JJ denotes gray
T24225 11126-11130 NN denotes line
T24226 11131-11140 VB denotes indicates
T24227 11141-11144 DT denotes the
T24228 11145-11147 NN denotes IC
T24229 11148-11158 NN denotes background
T24230 11159-11164 NN denotes level
T24231 11166-11169 DT denotes The
T24232 11170-11175 NN denotes error
T24233 11176-11180 NN denotes bars
T24234 11181-11185 VB denotes show
T24235 11186-11189 DT denotes the
T24236 11190-11195 NN denotes error
T24237 11196-11198 IN denotes of
T24238 11199-11202 DT denotes the
T24239 11203-11208 NN denotes mean.
T24240 11209-11210 -LRB- denotes (
T24241 11210-11211 NN denotes C
T24242 11211-11212 -RRB- denotes )
T24243 11213-11222 RB denotes Similarly
T24244 11222-11223 -COMMA- denotes ,
T24245 11224-11225 DT denotes a
T24246 11226-11229 NN denotes Th2
T24247 11230-11235 NN denotes clone
T24248 11236-11237 -LRB- denotes (
T24249 11237-11240 NN denotes BR8
T24250 11240-11241 -RRB- denotes )
T24251 11241-11242 -COMMA- denotes ,
T24252 11243-11249 JJ denotes CRTH2+
T24253 11250-11253 NN denotes Th2
T24254 11254-11259 NN denotes cells
T24255 11259-11260 -COMMA- denotes ,
T24256 11261-11275 NN denotes IL-4-secreting
T24257 11276-11281 NN denotes cells
T24258 11281-11282 -COMMA- denotes ,
T24259 11283-11286 CC denotes and
T24260 11287-11293 NN denotes memory
T24261 11294-11295 NN denotes T
T24262 11296-11301 NN denotes cells
T24263 11302-11303 -LRB- denotes (
T24264 11303-11309 NN denotes CD45RO
T24265 11309-11310 -RRB- denotes )
T24266 11311-11315 VB denotes were
T24267 11316-11323 VB denotes stained
T24268 11324-11327 IN denotes for
T24269 11328-11333 NN denotes FOXP3
T24270 11334-11337 CC denotes and
T24271 11338-11342 NN denotes IL-4
T24272 11344-11348 NN denotes Data
T24273 11349-11352 VB denotes are
T24274 11353-11367 JJ denotes representative
T24275 11368-11370 IN denotes of
T24276 11371-11376 CD denotes three
T24277 11377-11388 JJ denotes independent
T24278 11389-11400 NN denotes experiments
T24706 13213-13218 NN denotes FOXP3
T24707 13219-13228 NN denotes Induction
T24708 13229-13235 IN denotes During
T24709 13236-13239 DT denotes the
T24710 13240-13255 NN denotes Differentiation
T24711 13256-13263 NN denotes Process
T24712 13264-13265 -LRB- denotes (
T24713 13265-13266 NN denotes A
T24714 13266-13267 -RRB- denotes )
T24715 13268-13273 JJ denotes Human
T24716 13274-13285 JJ denotes CD4+CD45RA+
T24717 13286-13287 NN denotes T
T24718 13288-13293 NN denotes cells
T24719 13294-13298 VB denotes were
T24720 13299-13308 VB denotes activated
T24721 13309-13313 IN denotes with
T24722 13314-13325 JJ denotes plate-bound
T24723 13326-13339 NN denotes anti-CD3/CD28
T24724 13340-13342 IN denotes in
T24725 13343-13346 DT denotes the
T24726 13347-13355 NN denotes presence
T24727 13356-13358 IN denotes of
T24728 13359-13364 NN denotes TGF-β
T24729 13365-13366 -LRB- denotes (
T24730 13366-13367 CD denotes 5
T24731 13368-13373 NN denotes ng/ml
T24732 13373-13374 -RRB- denotes )
T24733 13375-13377 CC denotes or
T24734 13378-13382 NN denotes IL-4
T24735 13383-13384 -LRB- denotes (
T24736 13384-13386 CD denotes 25
T24737 13387-13392 NN denotes ng/ml
T24738 13392-13393 -RRB- denotes )
T24739 13395-13398 DT denotes The
T24740 13399-13404 NN denotes cells
T24741 13405-13409 VB denotes were
T24742 13410-13419 VB denotes harvested
T24743 13420-13422 IN denotes at
T24744 13423-13432 JJ denotes different
T24745 13433-13437 NN denotes time
T24746 13438-13444 NN denotes points
T24747 13444-13445 -COMMA- denotes ,
T24748 13446-13449 CC denotes and
T24749 13450-13454 NN denotes mRNA
T24750 13455-13458 VB denotes was
T24751 13459-13469 VB denotes quantified
T24752 13470-13472 IN denotes by
T24753 13473-13482 JJ denotes real-time
T24754 13483-13486 NN denotes PCR
T24755 13487-13490 IN denotes for
T24756 13491-13496 NN denotes FOXP3
T24757 13497-13500 CC denotes and
T24758 13501-13506 NN denotes GATA3
T24759 13507-13517 NN denotes expression
T24760 13519-13523 NN denotes Bars
T24761 13524-13528 VB denotes show
T24762 13529-13532 DT denotes the
T24763 13533-13537 JJ denotes mean
T24764 13538-13539 JJ denotes ±
T24765 13540-13542 NN denotes SD
T24766 13543-13545 IN denotes of
T24767 13546-13551 CD denotes three
T24768 13552-13563 JJ denotes independent
T24769 13564-13575 NN denotes experiments
T24770 13578-13579 -LRB- denotes (
T24771 13579-13580 NN denotes B
T24772 13580-13581 -RRB- denotes )
T24773 13582-13595 JJ denotes Intracellular
T24774 13596-13601 NN denotes GATA3
T24775 13602-13605 CC denotes and
T24776 13606-13611 NN denotes FOXP3
T24777 13612-13620 NN denotes staining
T24778 13621-13623 VB denotes is
T24779 13624-13629 VB denotes shown
T24780 13630-13635 IN denotes after
T24781 13636-13644 NN denotes exposure
T24782 13645-13647 IN denotes of
T24783 13648-13659 JJ denotes CD4+CD45RA+
T24784 13660-13661 NN denotes T
T24785 13662-13667 NN denotes cells
T24786 13668-13670 TO denotes to
T24787 13671-13686 VB denotes differentiating
T24788 13687-13697 NN denotes conditions
T24789 13698-13700 IN denotes as
T24790 13701-13703 IN denotes in
T24791 13704-13708 NN denotes part
T24792 13709-13710 NN denotes A
T24793 13711-13713 IN denotes of
T24794 13714-13717 DT denotes the
T24795 13718-13724 NN denotes figure
T24796 13726-13730 NN denotes Data
T24797 13731-13734 VB denotes are
T24798 13735-13749 JJ denotes representative
T24799 13750-13752 IN denotes of
T24800 13753-13758 CD denotes three
T24801 13759-13770 JJ denotes independent
T24802 13771-13782 NN denotes experiments
T25158 16498-16504 NN denotes Effect
T25159 16505-16507 IN denotes of
T25160 16508-16512 NN denotes IL-4
T25161 16513-16515 IN denotes on
T25162 16516-16521 NN denotes FOXP3
T25163 16522-16531 NN denotes Induction
T25164 16532-16533 -LRB- denotes (
T25165 16533-16534 NN denotes A
T25166 16534-16535 -RRB- denotes )
T25167 16536-16537 NN denotes A
T25168 16538-16549 JJ denotes statistical
T25169 16550-16558 NN denotes analysis
T25170 16559-16562 VB denotes was
T25171 16563-16572 VB denotes performed
T25172 16573-16577 IN denotes with
T25173 16578-16581 CD denotes six
T25174 16582-16588 NN denotes donors
T25175 16589-16591 IN denotes on
T25176 16592-16595 NN denotes day
T25177 16596-16597 CD denotes 5
T25178 16598-16599 -LRB- denotes (
T25179 16599-16604 NN denotes TGF-β
T25180 16605-16606 -LRB- denotes (
T25181 16606-16608 CD denotes 10
T25182 16609-16614 NN denotes ng/ml
T25183 16614-16615 -RRB- denotes )
T25184 16616-16619 CC denotes and
T25185 16620-16624 IN denotes with
T25186 16625-16627 CC denotes or
T25187 16628-16635 IN denotes without
T25188 16636-16640 NN denotes IL-4
T25189 16641-16642 -LRB- denotes (
T25190 16642-16645 CD denotes 100
T25191 16646-16651 NN denotes ng/ml
T25192 16651-16652 -RRB- denotes )
T25193 16652-16653 -RRB- denotes )
T25194 16653-16654 -COLON- denotes ;
T25195 16655-16660 NNP denotes Shown
T25196 16661-16663 VB denotes is
T25197 16664-16667 DT denotes the
T25198 16668-16672 NN denotes mean
T25199 16672-16673 -COMMA- denotes ,
T25200 16674-16677 CC denotes and
T25201 16678-16683 NN denotes error
T25202 16684-16688 NN denotes bars
T25203 16689-16698 VB denotes indicated
T25204 16699-16702 DT denotes the
T25205 16703-16705 NN denotes SD
T25206 16706-16708 IN denotes of
T25207 16709-16712 CD denotes six
T25208 16713-16719 NN denotes donors
T25209 16721-16732 JJ denotes Statistical
T25210 16733-16741 NN denotes analysis
T25211 16742-16745 VB denotes was
T25212 16746-16755 VB denotes performed
T25213 16756-16761 VB denotes using
T25214 16762-16765 DT denotes the
T25215 16766-16773 NNP denotes Dunnett
T25216 16774-16778 NN denotes test
T25217 16780-16791 JJ denotes Statistical
T25218 16792-16804 NN denotes significance
T25219 16805-16807 VB denotes is
T25220 16808-16817 VB denotes indicated
T25221 16818-16820 IN denotes by
T25222 16821-16830 NN denotes asterisks
T25223 16831-16832 -LRB- denotes (
T25224 16832-16834 NN denotes *p
T25225 16835-16836 NN denotes ≤
T25226 16837-16841 CD denotes 0.05
T25227 16841-16842 -COMMA- denotes ,
T25228 16843-16846 NN denotes **p
T25229 16847-16848 -COMMA- denotes ≤
T25230 16849-16853 CD denotes 0.01
T25231 16853-16854 -COMMA- denotes ,
T25232 16855-16862 NNP denotes Dunnett
T25233 16862-16863 -RRB- denotes )
T25234 16865-16866 -LRB- denotes (
T25235 16866-16867 NN denotes B
T25236 16867-16868 -RRB- denotes )
T25237 16869-16880 JJ denotes CD4+CD45RA+
T25238 16881-16886 NN denotes cells
T25239 16887-16891 VB denotes were
T25240 16892-16901 VB denotes activated
T25241 16902-16904 IN denotes in
T25242 16905-16908 DT denotes the
T25243 16909-16917 NN denotes presence
T25244 16918-16920 IN denotes of
T25245 16921-16922 DT denotes a
T25246 16923-16931 JJ denotes constant
T25247 16932-16945 NN denotes concentration
T25248 16946-16948 IN denotes of
T25249 16949-16954 NN denotes TGF-β
T25250 16955-16956 -LRB- denotes (
T25251 16956-16957 CD denotes 5
T25252 16958-16963 NN denotes ng/ml
T25253 16963-16964 -RRB- denotes )
T25254 16965-16969 IN denotes with
T25255 16970-16972 DT denotes an
T25256 16973-16983 VB denotes increasing
T25257 16984-16997 NN denotes concentration
T25258 16998-17000 IN denotes of
T25259 17001-17005 NN denotes IL-4
T25260 17005-17006 -COMMA- denotes ,
T25261 17007-17009 IN denotes as
T25262 17010-17019 VB denotes indicated
T25263 17021-17026 NN denotes Cells
T25264 17027-17031 VB denotes were
T25265 17032-17041 VB denotes harvested
T25266 17042-17045 IN denotes for
T25267 17046-17050 NN denotes mRNA
T25268 17051-17065 NN denotes quantification
T25269 17066-17071 IN denotes after
T25270 17072-17073 CD denotes 5
T25271 17074-17075 NN denotes d
T25272 17077-17078 -LRB- denotes (
T25273 17078-17079 NN denotes C
T25274 17079-17080 -RRB- denotes )
T25275 17081-17092 JJ denotes CD4+CD45RA+
T25276 17093-17098 NN denotes cells
T25277 17099-17103 VB denotes were
T25278 17104-17114 VB denotes stimulated
T25279 17115-17117 FW denotes in
T25280 17118-17123 FW denotes vitro
T25281 17124-17128 IN denotes with
T25282 17129-17140 JJ denotes plate-bound
T25283 17141-17154 NN denotes anti-CD3/CD28
T25284 17154-17155 -COMMA- denotes ,
T25285 17156-17161 NN denotes TGF-β
T25286 17162-17163 -LRB- denotes (
T25287 17163-17170 NN denotes 10ng/ml
T25288 17170-17171 -RRB- denotes )
T25289 17171-17172 -COMMA- denotes ,
T25290 17173-17176 CC denotes and
T25291 17177-17181 NN denotes IL-4
T25292 17182-17183 -LRB- denotes (
T25293 17183-17186 CD denotes 100
T25294 17187-17192 NN denotes ng/ml
T25295 17192-17193 -RRB- denotes )
T25296 17194-17196 IN denotes as
T25297 17197-17206 VB denotes indicated
T25298 17208-17213 IN denotes After
T25299 17214-17215 CD denotes 1
T25300 17216-17217 NN denotes h
T25301 17217-17218 -COMMA- denotes ,
T25302 17219-17223 NN denotes cell
T25303 17224-17231 NN denotes lysates
T25304 17232-17236 VB denotes were
T25305 17237-17245 VB denotes prepared
T25306 17246-17249 CC denotes and
T25307 17250-17258 VB denotes analyzed
T25308 17259-17261 IN denotes by
T25309 17262-17269 JJ denotes Western
T25310 17270-17274 NN denotes blot
T25311 17275-17278 IN denotes for
T25312 17279-17293 VB denotes phosphorylated
T25313 17294-17299 NN denotes SMAD2
T25314 17300-17303 CC denotes and
T25315 17304-17309 NN denotes STAT6
T25316 17311-17316 JJ denotes Total
T25317 17317-17322 NN denotes STAT6
T25318 17323-17326 CC denotes and
T25319 17327-17332 NN denotes GAPDH
T25320 17333-17339 VB denotes served
T25321 17340-17342 IN denotes as
T25322 17343-17351 JJ denotes internal
T25323 17352-17359 NN denotes control
T25324 17361-17362 -LRB- denotes (
T25325 17362-17363 NN denotes D
T25326 17363-17364 -RRB- denotes )
T25327 17365-17378 JJ denotes Intracellular
T25328 17379-17384 NN denotes GATA3
T25329 17385-17388 CC denotes and
T25330 17389-17394 NN denotes FOXP3
T25331 17395-17403 NN denotes staining
T25332 17404-17407 VB denotes are
T25333 17408-17413 VB denotes shown
T25334 17414-17419 IN denotes after
T25335 17420-17428 NN denotes exposure
T25336 17429-17431 IN denotes of
T25337 17432-17443 JJ denotes CD4+CD45RA+
T25338 17444-17445 NN denotes T
T25339 17446-17451 NN denotes cells
T25340 17452-17454 TO denotes to
T25341 17455-17459 NN denotes IL-4
T25342 17460-17462 IN denotes as
T25343 17463-17472 VB denotes described
T25344 17473-17476 IN denotes for
T25345 17477-17482 NN denotes panel
T25346 17483-17485 NNP denotes B.
T25347 17486-17490 NNP denotes Data
T25348 17491-17494 VB denotes are
T25349 17495-17509 JJ denotes representative
T25350 17510-17512 IN denotes of
T25351 17513-17518 CD denotes three
T25352 17519-17530 JJ denotes independent
T25353 17531-17542 NN denotes experiments
T25880 18665-18669 NN denotes IL-4
T25881 18670-18678 VB denotes Inhibits
T25882 18679-18693 JJ denotes TGF-β–Mediated
T25883 18694-18699 NN denotes iTreg
T25884 18700-18710 NNP denotes Commitment
T25885 18711-18723 JJ denotes CFSE-labeled
T25886 18724-18735 JJ denotes CD4+CD45RA+
T25887 18736-18741 NN denotes cells
T25888 18742-18746 VB denotes were
T25889 18747-18756 VB denotes activated
T25890 18757-18761 IN denotes with
T25891 18762-18773 JJ denotes plate-bound
T25892 18774-18787 NN denotes anti-CD3/CD28
T25893 18787-18788 -COMMA- denotes ,
T25894 18789-18794 NN denotes TGF-β
T25895 18794-18795 -COMMA- denotes ,
T25896 18796-18799 CC denotes and
T25897 18800-18804 NN denotes IL-4
T25898 18804-18805 -COMMA- denotes ,
T25899 18806-18808 IN denotes as
T25900 18809-18818 VB denotes indicated
T25901 18820-18825 IN denotes After
T25902 18826-18827 CD denotes 5
T25903 18828-18829 NN denotes d
T25904 18829-18830 -COMMA- denotes ,
T25905 18831-18836 NN denotes cells
T25906 18837-18841 VB denotes were
T25907 18842-18850 VB denotes analyzed
T25908 18851-18853 IN denotes by
T25909 18854-18858 NN denotes flow
T25910 18859-18868 NN denotes cytometry
T25911 18869-18870 -LRB- denotes (
T25912 18870-18871 NN denotes A
T25913 18871-18872 -RRB- denotes )
T25914 18873-18876 CC denotes and
T25915 18877-18884 NN denotes results
T25916 18885-18887 IN denotes of
T25917 18888-18891 CD denotes six
T25918 18892-18903 JJ denotes independent
T25919 18904-18915 NN denotes experiments
T25920 18916-18919 VB denotes are
T25921 18920-18925 VB denotes shown
T25922 18926-18928 IN denotes in
T25923 18929-18932 DT denotes the
T25924 18933-18936 NN denotes bar
T25925 18937-18942 NN denotes graph
T25926 18943-18948 IN denotes below
T25927 18949-18950 -LRB- denotes (
T25928 18950-18951 NN denotes B
T25929 18951-18952 -RRB- denotes )
T25930 18954-18965 JJ denotes Statistical
T25931 18966-18978 NN denotes significance
T25932 18979-18980 -LRB- denotes (
T25933 18980-18987 JJ denotes one-way
T25934 18988-18993 NNP denotes Anova
T25935 18993-18994 -COMMA- denotes ,
T25936 18995-19007 NNP denotes Newman-Keuls
T25937 19007-19008 -RRB- denotes )
T25938 19009-19011 VB denotes is
T25939 19012-19021 VB denotes indicated
T25940 19022-19024 IN denotes by
T25941 19025-19034 NN denotes asterisks
T25942 19035-19036 -LRB- denotes (
T25943 19036-19039 NN denotes **p
T25944 19040-19041 SYM denotes ≤
T25945 19042-19046 CD denotes 0.01
T25946 19046-19047 -COMMA- denotes ,
T25947 19048-19052 NN denotes ***p
T25948 19053-19054 -COLON- denotes ≤
T25949 19055-19060 CD denotes 0.001
T25950 19060-19061 -RRB- denotes )
T25951 19061-19062 -LRB- denotes .
T25952 19063-19064 NN denotes (
T25953 19064-19065 -RRB- denotes C
T25954 19065-19072 JJ denotes ) Kinet
T25955 19072-19080 NN denotes ic analy
T25956 19080-19082 IN denotes si
T25957 19082-19095 JJ denotes s of intracel
T25958 19095-19100 NN denotes lular
T25959 19101-19104 CC denotes GAT
T25960 19104-19109 NN denotes A3 an
T25961 19109-19117 NN denotes d FOXP3
T25962 19117-19119 VB denotes st
T25963 19119-19124 VB denotes ainin
T25964 19124-19126 IN denotes g
T25965 19126-19131 NN denotes is sh
T25966 19131-19132 NN denotes o
T25967 19132-19141 VB denotes wn in pan
T25968 19141-19149 NN denotes el C fol
T25969 19149-19151 IN denotes lo
T25970 19151-19162 JJ denotes wing exposu
T25971 19162-19163 NN denotes r
T25972 19163-19168 NN denotes e of
T25973 19168-19170 TO denotes CD
T25974 19170-19181 JJ denotes 4+CD45RA+ T
T25975 19182-19183 -COMMA- denotes c
T25976 19183-19187 NN denotes ells
T25977 19188-19191 CC denotes to
T25978 19191-19196 NN denotes anti-
T25979 19220-19224 NN denotes Data
T25980 19225-19228 VB denotes are
T25981 19229-19243 JJ denotes representative
T25982 19244-19246 IN denotes of
T25983 19247-19252 CD denotes three
T25984 19253-19264 JJ denotes independent
T25985 19265-19276 NN denotes experiments
T26347 22450-22455 NN denotes GATA3
T26348 22456-22460 VB denotes Acts
T26349 22461-22463 IN denotes as
T26350 22464-22465 DT denotes a
T26351 22466-22474 JJ denotes Negative
T26352 22475-22484 NN denotes Regulator
T26353 22485-22487 IN denotes of
T26354 22488-22493 NN denotes FOXP3
T26355 22494-22504 NN denotes Expression
T26356 22505-22506 -LRB- denotes (
T26357 22506-22507 NN denotes A
T26358 22507-22508 -RRB- denotes )
T26359 22509-22514 JJ denotes Human
T26360 22515-22520 JJ denotes naive
T26361 22521-22530 JJ denotes CD4+CDRA+
T26362 22531-22532 NN denotes T
T26363 22533-22538 NN denotes cells
T26364 22539-22543 VB denotes were
T26365 22544-22554 VB denotes transduced
T26366 22555-22559 IN denotes with
T26367 22560-22561 CD denotes 0
T26368 22561-22562 -COMMA- denotes ,
T26369 22563-22565 CD denotes 20
T26370 22565-22566 -COMMA- denotes ,
T26371 22567-22570 CD denotes 100
T26372 22570-22571 -COMMA- denotes ,
T26373 22572-22575 CC denotes and
T26374 22576-22579 CD denotes 500
T26375 22580-22582 NN denotes nM
T26376 22583-22585 IN denotes of
T26377 22586-22595 NN denotes TAT-GATA3
T26378 22596-22603 NN denotes protein
T26379 22603-22604 -COMMA- denotes ,
T26380 22605-22608 CC denotes and
T26381 22609-22622 JJ denotes intracellular
T26382 22623-22631 NN denotes presence
T26383 22632-22634 IN denotes of
T26384 22635-22640 NN denotes GATA3
T26385 22641-22644 VB denotes was
T26386 22645-22653 VB denotes analyzed
T26387 22654-22659 VB denotes using
T26388 22660-22664 NN denotes FACS
T26389 22665-22674 VB denotes following
T26390 22675-22688 JJ denotes anti-CD3/CD28
T26391 22689-22699 NN denotes activation
T26392 22700-22702 IN denotes of
T26393 22703-22706 DT denotes the
T26394 22707-22712 NN denotes cells
T26395 22714-22726 JJ denotes GFP-positive
T26396 22727-22732 NN denotes cells
T26397 22733-22737 VB denotes were
T26398 22738-22743 VB denotes gated
T26399 22744-22747 CC denotes and
T26400 22748-22756 VB denotes analyzed
T26401 22757-22760 IN denotes for
T26402 22761-22774 JJ denotes intracellular
T26403 22775-22780 NN denotes FOXP3
T26404 22781-22791 NN denotes expression
T26405 22792-22801 VB denotes following
T26406 22802-22803 DT denotes a
T26407 22804-22807 JJ denotes 2-d
T26408 22808-22818 NN denotes incubation
T26409 22819-22825 NN denotes period
T26410 22826-22827 -LRB- denotes (
T26411 22827-22832 JJ denotes lower
T26412 22833-22838 NN denotes panel
T26413 22838-22839 -RRB- denotes )
T26414 22841-22845 NN denotes Data
T26415 22846-22849 VB denotes are
T26416 22850-22864 JJ denotes representative
T26417 22865-22867 IN denotes of
T26418 22868-22872 CD denotes four
T26419 22873-22884 JJ denotes independent
T26420 22885-22896 NN denotes experiments
T26421 22898-22899 -LRB- denotes (
T26422 22899-22900 NN denotes B
T26423 22900-22901 -RRB- denotes )
T26424 22902-22911 NN denotes CD4+CD25−
T26425 22912-22913 NN denotes T
T26426 22914-22919 NN denotes cells
T26427 22920-22924 VB denotes were
T26428 22925-22933 VB denotes isolated
T26429 22934-22938 IN denotes from
T26430 22939-22946 NN denotes D011.10
T26431 22947-22950 CC denotes and
T26432 22951-22968 NN denotes D011.10xCD2-GATA3
T26433 22969-22973 NN denotes mice
T26434 22974-22977 CC denotes and
T26435 22978-22985 VB denotes treated
T26436 22986-22990 IN denotes with
T26437 22991-22994 NN denotes OVA
T26438 22995-22998 CC denotes and
T26439 22999-23004 NN denotes TGF-β
T26440 23005-23008 IN denotes for
T26441 23009-23011 CD denotes 96
T26442 23012-23013 NN denotes h
T26443 23015-23022 NN denotes Surface
T26444 23023-23026 NN denotes CD4
T26445 23027-23030 CC denotes and
T26446 23031-23044 JJ denotes intracellular
T26447 23045-23050 NN denotes FOXP3
T26448 23051-23055 VB denotes were
T26449 23056-23064 VB denotes measured
T26450 23065-23067 IN denotes by
T26451 23068-23072 NN denotes FACS
T26452 23074-23079 DT denotes These
T26453 23080-23084 NN denotes data
T26454 23085-23088 VB denotes are
T26455 23089-23103 JJ denotes representative
T26456 23104-23106 IN denotes of
T26457 23107-23112 CD denotes three
T26458 23113-23124 JJ denotes independent
T26459 23125-23136 NN denotes experiments
T26460 23138-23139 -LRB- denotes (
T26461 23139-23140 NN denotes C
T26462 23140-23141 -RRB- denotes )
T26463 23142-23145 DT denotes The
T26464 23146-23151 NN denotes cells
T26465 23152-23159 VB denotes treated
T26466 23160-23162 IN denotes as
T26467 23163-23165 IN denotes in
T26468 23166-23167 -LRB- denotes (
T26469 23167-23168 NN denotes B
T26470 23168-23169 -RRB- denotes )
T26471 23170-23174 VB denotes were
T26472 23175-23184 VB denotes harvested
T26473 23185-23188 CC denotes and
T26474 23189-23193 NN denotes mRNA
T26475 23194-23197 VB denotes was
T26476 23198-23208 VB denotes quantified
T26477 23209-23211 IN denotes by
T26478 23212-23221 JJ denotes real-time
T26479 23222-23225 NN denotes PCR
T26480 23226-23229 IN denotes for
T26481 23230-23235 NN denotes SMAD7
T26482 23236-23239 CC denotes and
T26483 23240-23245 NN denotes TGF-β
T26484 23246-23256 NN denotes expression
T26485 23258-23262 NN denotes Bars
T26486 23263-23267 VB denotes show
T26487 23268-23271 DT denotes the
T26488 23272-23276 JJ denotes mean
T26489 23277-23278 JJ denotes ±
T26490 23279-23281 NN denotes SD
T26491 23282-23284 IN denotes of
T26492 23285-23290 CD denotes three
T26493 23291-23302 JJ denotes independent
T26494 23303-23314 NN denotes experiments
T26944 25937-25942 NN denotes GATA3
T26945 25943-25952 VB denotes Represses
T26946 25953-25956 DT denotes the
T26947 25957-25962 JJ denotes Human
T26948 25963-25968 NN denotes FOXP3
T26949 25969-25977 NN denotes Promoter
T26950 25978-25979 -LRB- denotes (
T26951 25979-25980 NN denotes A
T26952 25980-25981 -RRB- denotes )
T26953 25982-25988 NN denotes Jurkat
T26954 25988-25989 -COMMA- denotes ,
T26955 25990-25993 NN denotes Th2
T26956 25994-25999 NN denotes cells
T26957 25999-26000 -COMMA- denotes ,
T26958 26001-26004 CC denotes and
T26959 26005-26010 JJ denotes human
T26960 26011-26018 JJ denotes primary
T26961 26019-26022 NN denotes CD4
T26962 26023-26028 NN denotes cells
T26963 26029-26033 VB denotes were
T26964 26034-26045 VB denotes transfected
T26965 26046-26050 IN denotes with
T26966 26051-26053 DT denotes an
T26967 26054-26059 JJ denotes empty
T26968 26060-26066 NN denotes vector
T26969 26067-26068 -LRB- denotes (
T26970 26068-26072 NN denotes pGL3
T26971 26073-26078 JJ denotes basic
T26972 26078-26079 -RRB- denotes )
T26973 26080-26082 CC denotes or
T26974 26083-26084 DT denotes a
T26975 26085-26091 NN denotes vector
T26976 26092-26102 VB denotes containing
T26977 26103-26106 DT denotes the
T26978 26107-26115 JJ denotes putative
T26979 26116-26121 NN denotes FOXP3
T26980 26122-26130 NN denotes promoter
T26981 26131-26137 NN denotes region
T26982 26138-26143 VB denotes fused
T26983 26144-26146 TO denotes to
T26984 26147-26150 DT denotes the
T26985 26151-26161 NN denotes luciferase
T26986 26162-26170 NN denotes reporter
T26987 26171-26175 NN denotes gene
T26988 26177-26181 NN denotes Bars
T26989 26182-26186 VB denotes show
T26990 26187-26190 DT denotes the
T26991 26191-26195 JJ denotes mean
T26992 26196-26197 JJ denotes ±
T26993 26198-26200 NN denotes SD
T26994 26201-26203 IN denotes of
T26995 26204-26213 JJ denotes arbitrary
T26996 26214-26219 JJ denotes light
T26997 26220-26225 NN denotes units
T26998 26226-26236 VB denotes normalized
T26999 26237-26240 IN denotes for
T27000 26241-26248 NN denotes renilla
T27001 26249-26259 NN denotes luciferase
T27002 26260-26262 IN denotes of
T27003 26263-26267 CD denotes four
T27004 26268-26279 JJ denotes independent
T27005 26280-26291 NN denotes experiments
T27006 26291-26292 -COLON- denotes ;
T27007 26293-26300 NN denotes samples
T27008 26301-26305 VB denotes were
T27009 26306-26314 VB denotes measured
T27010 26315-26317 IN denotes in
T27011 26318-26329 NN denotes triplicates
T27012 26331-26332 -LRB- denotes (
T27013 26332-26333 NN denotes B
T27014 26333-26334 -RRB- denotes )
T27015 26335-26340 JJ denotes Naive
T27016 26341-26344 NN denotes CD4
T27017 26345-26346 NN denotes T
T27018 26347-26352 NN denotes cells
T27019 26353-26357 VB denotes were
T27020 26358-26369 VB denotes transfected
T27021 26370-26374 IN denotes with
T27022 26375-26378 DT denotes the
T27023 26379-26384 NN denotes FOXP3
T27024 26385-26393 NN denotes promoter
T27025 26394-26402 NN denotes reporter
T27026 26403-26412 NN denotes construct
T27027 26413-26421 RB denotes together
T27028 26422-26426 IN denotes with
T27029 26427-26428 DT denotes a
T27030 26429-26434 NN denotes GATA3
T27031 26435-26445 NN denotes expression
T27032 26446-26452 NN denotes vector
T27033 26453-26455 CC denotes or
T27034 26456-26458 DT denotes an
T27035 26459-26464 JJ denotes empty
T27036 26465-26471 NN denotes vector
T27037 26473-26477 NN denotes Bars
T27038 26478-26482 VB denotes show
T27039 26483-26486 DT denotes the
T27040 26487-26491 JJ denotes mean
T27041 26492-26493 JJ denotes ±
T27042 26494-26496 NN denotes SD
T27043 26497-26499 IN denotes of
T27044 26500-26505 CD denotes three
T27045 26506-26517 JJ denotes independent
T27046 26518-26529 NN denotes experiments
T27047 26531-26532 -LRB- denotes (
T27048 26532-26533 NN denotes C
T27049 26533-26534 -RRB- denotes )
T27050 26535-26540 JJ denotes Naive
T27051 26541-26542 -LRB- denotes (
T27052 26542-26546 JJ denotes left
T27053 26547-26552 NN denotes panel
T27054 26552-26553 -RRB- denotes )
T27055 26554-26556 CC denotes or
T27056 26557-26563 NN denotes memory
T27057 26564-26565 -LRB- denotes (
T27058 26565-26570 JJ denotes right
T27059 26571-26576 NN denotes panel
T27060 26576-26577 -RRB- denotes )
T27061 26578-26581 NN denotes CD4
T27062 26582-26583 NN denotes T
T27063 26584-26589 NN denotes cells
T27064 26590-26594 VB denotes were
T27065 26595-26606 VB denotes transfected
T27066 26607-26611 IN denotes with
T27067 26612-26621 JJ denotes wild-type
T27068 26622-26624 CC denotes or
T27069 26625-26626 DT denotes a
T27070 26627-26632 NN denotes GATA3
T27071 26633-26640 VB denotes mutated
T27072 26641-26650 NN denotes 511-FOXP3
T27073 26651-26659 NN denotes promoter
T27074 26660-26668 NN denotes reporter
T27075 26669-26678 NN denotes construct
T27076 26679-26682 CC denotes and
T27077 26683-26692 VB denotes activated
T27078 26693-26697 IN denotes with
T27079 26698-26701 NN denotes PMA
T27080 26702-26705 CC denotes and
T27081 26706-26715 NN denotes ionomycin
T27082 26717-26721 NN denotes Bars
T27083 26722-26726 VB denotes show
T27084 26727-26730 DT denotes the
T27085 26731-26735 JJ denotes mean
T27086 26736-26737 JJ denotes ±
T27087 26738-26740 NN denotes SD
T27088 26741-26743 IN denotes of
T27089 26744-26753 JJ denotes arbitrary
T27090 26754-26759 JJ denotes light
T27091 26760-26765 NN denotes units
T27092 26766-26776 VB denotes normalized
T27093 26777-26780 IN denotes for
T27094 26781-26788 NN denotes renilla
T27095 26789-26799 NN denotes luciferase
T27096 26800-26802 IN denotes of
T27097 26803-26808 CD denotes eight
T27098 26809-26820 JJ denotes independent
T27099 26821-26832 NN denotes experiments
T27100 26832-26833 -COLON- denotes ;
T27101 26834-26841 NN denotes samples
T27102 26842-26846 VB denotes were
T27103 26847-26855 VB denotes measured
T27104 26856-26858 IN denotes in
T27105 26859-26870 NN denotes triplicates
T27106 26872-26873 -LRB- denotes (
T27107 26873-26874 NN denotes D
T27108 26874-26875 -RRB- denotes )
T27109 26876-26883 JJ denotes Nuclear
T27110 26884-26892 NN denotes extracts
T27111 26893-26897 VB denotes were
T27112 26898-26906 VB denotes prepared
T27113 26907-26911 IN denotes from
T27114 26912-26915 NN denotes HEK
T27115 26916-26921 NN denotes cells
T27116 26922-26933 VB denotes transfected
T27117 26934-26938 IN denotes with
T27118 26939-26944 NN denotes GATA3
T27119 26945-26947 CC denotes or
T27120 26948-26950 DT denotes an
T27121 26951-26956 JJ denotes empty
T27122 26957-26963 NN denotes vector
T27123 26963-26964 -COMMA- denotes ,
T27124 26965-26966 -LRB- denotes (
T27125 26966-26967 NN denotes E
T27126 26967-26968 -RRB- denotes )
T27127 26969-26972 NN denotes Th1
T27128 26972-26973 -COMMA- denotes ,
T27129 26974-26977 NN denotes Th2
T27130 26977-26978 -COMMA- denotes ,
T27131 26979-26981 CC denotes or
T27132 26982-26987 NN denotes iTreg
T27133 26988-26993 NN denotes cells
T27134 26994-26997 CC denotes and
T27135 26998-27005 NN denotes binding
T27136 27006-27013 NN denotes factors
T27137 27014-27026 VB denotes precipitated
T27138 27027-27032 VB denotes using
T27139 27033-27045 VB denotes biotinylated
T27140 27046-27062 NN denotes oligonucleotides
T27141 27064-27067 DT denotes The
T27142 27068-27098 NN denotes oligonucleotides–transcription
T27143 27099-27105 NN denotes factor
T27144 27106-27115 NN denotes complexes
T27145 27116-27120 VB denotes were
T27146 27121-27130 VB denotes separated
T27147 27131-27133 IN denotes on
T27148 27134-27135 DT denotes a
T27149 27136-27144 NN denotes SDS-PAGE
T27150 27145-27148 NN denotes gel
T27151 27150-27153 DT denotes The
T27152 27154-27161 NN denotes amounts
T27153 27162-27164 IN denotes of
T27154 27165-27170 NN denotes GATA3
T27155 27171-27178 NN denotes protein
T27156 27179-27181 IN denotes in
T27157 27182-27185 DT denotes the
T27158 27186-27198 NN denotes precipitates
T27159 27199-27203 VB denotes were
T27160 27204-27212 VB denotes assessed
T27161 27213-27215 IN denotes by
T27162 27216-27230 NN denotes immunoblotting
T27163 27231-27235 IN denotes with
T27164 27236-27246 JJ denotes anti-GATA3
T27165 27247-27250 NN denotes mAb
T27166 27252-27257 JJ denotes Total
T27167 27258-27265 JJ denotes nuclear
T27168 27266-27274 NN denotes extracts
T27169 27275-27279 VB denotes were
T27170 27280-27284 RB denotes also
T27171 27285-27288 VB denotes run
T27172 27289-27291 IN denotes as
T27173 27292-27300 NN denotes controls
T27174 27302-27306 NN denotes Data
T27175 27307-27310 VB denotes are
T27176 27311-27325 JJ denotes representative
T27177 27326-27328 IN denotes of
T27178 27329-27334 CD denotes three
T27179 27335-27344 JJ denotes different
T27180 27345-27356 NN denotes experiments
T27181 27358-27359 -LRB- denotes (
T27182 27359-27360 NN denotes F
T27183 27360-27361 -RRB- denotes )
T27184 27362-27367 NN denotes iTreg
T27185 27368-27370 CC denotes or
T27186 27371-27374 NN denotes Th2
T27187 27375-27380 NN denotes cells
T27188 27381-27385 VB denotes were
T27189 27386-27394 VB denotes analyzed
T27190 27395-27397 IN denotes by
T27191 27398-27402 NN denotes ChIP
T27192 27403-27406 IN denotes for
T27193 27407-27412 NN denotes GATA3
T27194 27413-27420 NN denotes binding
T27195 27421-27423 TO denotes to
T27196 27424-27427 DT denotes the
T27197 27428-27433 NN denotes FOXP3
T27198 27434-27442 NN denotes promoter
T27199 27444-27447 DT denotes The
T27200 27448-27455 NN denotes “input”
T27201 27456-27466 VB denotes represents
T27202 27467-27470 NN denotes PCR
T27203 27471-27484 NN denotes amplification
T27204 27485-27487 IN denotes of
T27205 27488-27491 DT denotes the
T27206 27492-27497 JJ denotes total
T27207 27498-27504 NN denotes sample
T27208 27504-27505 -COMMA- denotes ,
T27209 27506-27511 WDT denotes which
T27210 27512-27515 VB denotes was
T27211 27516-27519 RB denotes not
T27212 27520-27529 VB denotes subjected
T27213 27530-27532 TO denotes to
T27214 27533-27536 DT denotes any
T27215 27537-27550 NN denotes precipitation
T27216 27552-27559 NN denotes Results
T27217 27560-27563 VB denotes are
T27218 27564-27578 JJ denotes representative
T27219 27579-27581 IN denotes of
T27220 27582-27587 CD denotes three
T27221 27588-27599 JJ denotes independent
T27222 27600-27611 NN denotes experiments
T19522 38861-38868 NN denotes Cloning
T19523 38869-38871 IN denotes of
T19524 38872-38875 DT denotes the
T19525 38876-38881 NN denotes FOXP3
T19526 38882-38890 NN denotes promoter
T19527 38891-38894 CC denotes and
T19528 38895-38907 NN denotes construction
T19529 38908-38910 IN denotes of
T19530 38911-38917 JJ denotes mutant
T19531 38918-38928 NN denotes constructs
T19532 38930-38933 DT denotes The
T19533 38934-38939 NN denotes FOXP3
T19534 38940-38948 NN denotes promoter
T19535 38949-38952 VB denotes was
T19536 38953-38959 VB denotes cloned
T20051 39973-39976 NN denotes RNA
T20052 39977-39986 NN denotes isolation
T20053 39987-39990 CC denotes and
T20054 39991-39995 NN denotes cDNA
T20055 39996-40005 NN denotes synthesis
T20056 40007-40010 NN denotes RNA
T20057 40011-40014 VB denotes was
T20058 40015-40023 VB denotes isolated
T20059 40024-40029 VB denotes using
T20060 40030-40033 DT denotes the
T20061 40034-40040 NNP denotes RNeasy
T20062 40041-40045 NNP denotes Mini
T20063 40046-40049 NNP denotes Kit
T20064 40050-40051 -LRB- denotes (
T20065 40051-40057 NN denotes Qiagen
T20066 40057-40058 -RRB- denotes )
T20067 40059-40068 VB denotes according
T20068 40069-40071 TO denotes to
T20069 40072-40075 DT denotes the
T20070 40076-40088 NN denotes manufacturer
T20071 40088-40090 POS denotes 's
T20072 40091-40099 NN denotes protocol
T20073 40101-40108 JJ denotes Reverse
T20074 40109-40122 NN denotes transcription
T20075 40123-40125 IN denotes of
T20076 40126-40131 JJ denotes human
T20077 40132-40139 NN denotes samples
T20078 40140-40143 VB denotes was
T20079 40144-40153 VB denotes performed
R44 T109 T107 arg1Of Responses,GATA3-Driven
R45 T109 T108 arg1Of Responses,Th2
R46 T109 T110 arg1Of Responses,Inhibit
R47 T113 T111 arg1Of Expression,TGF-β1–Induced
R48 T113 T112 arg1Of Expression,FOXP3
R49 T113 T114 arg1Of Expression,and
R50 T114 T110 arg2Of and,Inhibit
R51 T116 T114 arg2Of Formation,and
R52 T116 T115 arg1Of Formation,the
R53 T116 T117 arg1Of Formation,of
R54 T120 T117 arg2Of Cells,of
R55 T120 T118 arg1Of Cells,Regulatory
R56 T120 T119 arg1Of Cells,T
R57 T122 T121 arg1Of factors,Transcription
R58 T122 T123 arg1Of factors,act
R59 T122 T127 arg1Of factors,induce
R60 T123 T124 arg1Of act,in
R61 T123 T126 modOf act,to
R62 T123 T143 arg1Of act,and
R63 T125 T124 arg2Of concert,in
R64 T127 T126 arg1Of induce,to
R65 T129 T127 arg2Of commitment,induce
R66 T129 T128 arg1Of commitment,lineage
R67 T129 T130 arg1Of commitment,towards
R68 T131 T132 arg1Of Th1,","
R69 T132 T135 arg1Of ",",or
R70 T133 T132 arg2Of Th2,","
R71 T135 T134 arg1Of or,","
R72 T136 T135 arg2Of T,or
R73 T139 T138 arg2Of Treg,(
R74 T140 T138 arg3Of ),(
R75 T141 T130 arg2Of cells,towards
R76 T141 T131 arg1Of cells,Th1
R77 T141 T133 arg1Of cells,Th2
R78 T141 T136 arg1Of cells,T
R79 T141 T137 arg1Of cells,regulatory
R80 T141 T138 arg1Of cells,(
R81 T143 T142 arg1Of and,","
R82 T146 T144 arg1Of mechanisms,their
R83 T146 T145 arg1Of mechanisms,counter-regulatory
R84 T146 T147 arg1Of mechanisms,were
R85 T146 T148 arg2Of mechanisms,shown
R86 T146 T150 arg1Of mechanisms,be
R87 T146 T151 arg1Of mechanisms,critical
R88 T148 T143 arg2Of shown,and
R89 T148 T147 arg2Of shown,were
R90 T150 T148 arg3Of be,shown
R91 T150 T149 arg1Of be,to
R92 T151 T150 arg2Of critical,be
R93 T151 T152 arg1Of critical,for
R94 T153 T152 arg2Of polarization,for
R95 T153 T154 arg1Of polarization,between
R96 T155 T156 arg1Of Th1,and
R97 T157 T156 arg2Of Th2,and
R98 T158 T154 arg2Of phenotypes,between
R99 T158 T155 arg1Of phenotypes,Th1
R100 T158 T157 arg1Of phenotypes,Th2
R101 T159 T160 arg1Of FOXP3,is
R102 T160 T179 arg1Of is,;
R103 T164 T160 arg2Of factor,is
R104 T164 T161 arg1Of factor,an
R105 T164 T162 arg1Of factor,essential
R106 T164 T163 arg1Of factor,transcription
R107 T164 T165 arg1Of factor,for
R108 T166 T167 arg1Of natural,","
R109 T168 T167 arg2Of thymus-derived,","
R110 T170 T166 arg1Of nTreg,natural
R111 T170 T168 arg1Of nTreg,thymus-derived
R112 T170 T169 arg2Of nTreg,(
R113 T170 T172 arg1Of nTreg,and
R114 T171 T169 arg3Of ),(
R115 T172 T165 arg2Of and,for
R116 T176 T175 arg2Of iTreg,(
R117 T177 T175 arg3Of ),(
R118 T178 T172 arg2Of commitment,and
R119 T178 T173 arg1Of commitment,inducible
R120 T178 T174 arg1Of commitment,Treg
R121 T178 T175 arg1Of commitment,(
R122 T183 T182 arg1Of mechanisms,the
R123 T183 T184 arg1Of mechanisms,regulating
R124 T183 T187 arg1Of mechanisms,are
R125 T183 T190 arg1Of mechanisms,unknown
R126 T186 T184 arg2Of expression,regulating
R127 T186 T185 arg1Of expression,its
R128 T187 T179 arg2Of are,;
R129 T187 T180 arg1Of are,however
R130 T187 T181 arg1Of are,","
R131 T187 T189 arg1Of are,yet
R132 T189 T188 arg1Of yet,as
R133 T190 T187 arg2Of unknown,are
R134 T191 T192 arg1Of We,describe
R135 T194 T192 arg2Of mechanism,describe
R136 T194 T193 arg1Of mechanism,a
R137 T194 T195 arg1Of mechanism,controlling
R138 T197 T195 arg2Of polarization,controlling
R139 T197 T196 arg1Of polarization,iTreg
R140 T197 T198 arg1Of polarization,","
R141 T197 T199 arg1Of polarization,which
R142 T197 T200 arg1Of polarization,is
R143 T197 T201 arg2Of polarization,overruled
R144 T201 T200 arg2Of overruled,is
R145 T206 T201 arg1Of pathway,overruled
R146 T206 T202 arg2Of pathway,by
R147 T206 T203 arg1Of pathway,the
R148 T206 T204 arg1Of pathway,Th2
R149 T206 T205 arg1Of pathway,differentiation
R150 T207 T208 arg1Of We,demonstrated
R151 T210 T211 arg1Of interleukin,4
R152 T210 T212 arg1Of interleukin,(
R153 T210 T215 arg1Of interleukin,present
R154 T210 T223 arg1Of interleukin,inhibits
R155 T213 T212 arg2Of IL-4,(
R156 T214 T212 arg3Of ),(
R157 T215 T216 arg1Of present,at
R158 T218 T216 arg2Of time,at
R159 T218 T217 arg1Of time,the
R160 T218 T219 arg1Of time,of
R161 T222 T219 arg2Of priming,of
R162 T222 T220 arg1Of priming,T
R163 T222 T221 arg1Of priming,cell
R164 T223 T208 arg2Of inhibits,demonstrated
R165 T223 T209 arg1Of inhibits,that
R166 T224 T223 arg2Of FOXP3,inhibits
R167 T227 T225 arg1Of mechanism,This
R168 T227 T226 arg1Of mechanism,inhibitory
R169 T227 T228 arg1Of mechanism,was
R170 T227 T230 arg2Of mechanism,confirmed
R171 T230 T228 arg2Of confirmed,was
R172 T230 T229 arg1Of confirmed,also
R173 T230 T231 arg1Of confirmed,in
R174 T230 T235 arg1Of confirmed,in
R175 T231 T234 arg1Of in,and
R176 T233 T231 arg2Of cells,in
R177 T233 T232 arg1Of cells,Th2
R178 T235 T234 arg2Of in,and
R179 T237 T235 arg2Of cells,in
R180 T237 T236 arg1Of cells,T
R181 T237 T238 arg1Of cells,of
R182 T240 T238 arg2Of mice,of
R183 T240 T239 arg1Of mice,transgenic
R184 T240 T241 arg1Of mice,overexpressing
R185 T240 T246 arg1Of mice,","
R186 T240 T247 arg1Of mice,which
R187 T240 T248 arg1Of mice,are
R188 T240 T249 arg2Of mice,shown
R189 T240 T251 arg1Of mice,be
R190 T240 T252 arg1Of mice,deficient
R191 T241 T243 arg1Of overexpressing,in
R192 T242 T241 arg2Of GATA-3,overexpressing
R193 T245 T243 arg2Of cells,in
R194 T245 T244 arg1Of cells,T
R195 T249 T248 arg2Of shown,are
R196 T251 T249 arg3Of be,shown
R197 T251 T250 arg1Of be,to
R198 T252 T251 arg2Of deficient,be
R199 T252 T253 arg1Of deficient,in
R200 T254 T253 arg2Of transforming,in
R201 T258 T257 arg2Of TGF,(
R202 T259 T257 arg3Of ),(
R203 T262 T254 arg2Of induction,transforming
R204 T262 T255 arg1Of induction,growth
R205 T262 T256 arg1Of induction,factor
R206 T262 T257 arg1Of induction,(
R207 T262 T260 arg1Of induction,-β–mediated
R208 T262 T261 arg1Of induction,FOXP3
R209 T264 T263 arg1Of inhibition,This
R210 T264 T265 arg1Of inhibition,is
R211 T264 T266 arg2Of inhibition,mediated
R212 T266 T265 arg2Of mediated,is
R213 T269 T266 arg1Of binding,mediated
R214 T269 T267 arg2Of binding,by
R215 T269 T268 arg1Of binding,direct
R216 T269 T270 arg1Of binding,of
R217 T269 T272 arg1Of binding,to
R218 T269 T276 arg1Of binding,","
R219 T269 T277 arg1Of binding,which
R220 T269 T278 arg1Of binding,represses
R221 T271 T270 arg2Of GATA3,of
R222 T275 T272 arg2Of promoter,to
R223 T275 T273 arg1Of promoter,the
R224 T275 T274 arg1Of promoter,FOXP3
R225 T281 T278 arg2Of process,represses
R226 T281 T279 arg1Of process,its
R227 T281 T280 arg1Of process,transactivation
R228 T285 T284 arg1Of study,this
R229 T285 T286 arg1Of study,provides
R230 T286 T282 arg1Of provides,Therefore
R231 T286 T283 arg1Of provides,","
R232 T289 T286 arg2Of understanding,provides
R233 T289 T287 arg1Of understanding,a
R234 T289 T288 arg1Of understanding,new
R235 T289 T290 arg1Of understanding,of
R236 T289 T293 arg1Of understanding,","
R237 T289 T294 arg2Of understanding,controlled
R238 T292 T290 arg2Of development,of
R239 T292 T291 arg1Of development,tolerance
R240 T300 T294 arg1Of response,controlled
R241 T300 T295 arg2Of response,by
R242 T300 T296 arg1Of response,a
R243 T300 T297 arg1Of response,type
R244 T300 T298 arg1Of response,2
R245 T300 T299 arg1Of response,immune
R246 T302 T301 arg1Of treatment,IL-4
R247 T302 T303 arg1Of treatment,in
R248 T302 T305 arg1Of treatment,reduces
R249 T302 T310 arg1Of treatment,highlighting
R250 T304 T303 arg2Of mice,in
R251 T305 T309 arg1Of reduces,","
R252 T305 T310 modOf reduces,highlighting
R253 T308 T305 arg2Of frequency,reduces
R254 T308 T306 arg1Of frequency,iTreg
R255 T308 T307 arg1Of frequency,cell
R256 T313 T312 arg1Of approaches,therapeutic
R257 T313 T314 arg1Of approaches,that
R258 T313 T315 arg1Of approaches,target
R259 T313 T319 arg1Of approaches,might
R260 T313 T320 arg1Of approaches,provide
R261 T316 T317 arg1Of IL-4,or
R262 T317 T315 arg2Of or,target
R263 T318 T317 arg2Of GATA3,or
R264 T320 T310 arg2Of provide,highlighting
R265 T320 T311 arg1Of provide,that
R266 T320 T319 arg2Of provide,might
R267 T320 T328 arg1Of provide,in
R268 T323 T320 arg2Of strategies,provide
R269 T323 T321 arg1Of strategies,new
R270 T323 T322 arg1Of strategies,preventive
R271 T323 T324 arg1Of strategies,facilitating
R272 T326 T324 arg2Of induction,facilitating
R273 T326 T325 arg1Of induction,tolerance
R274 T328 T327 arg1Of in,particularly
R275 T330 T328 arg2Of diseases,in
R276 T330 T329 arg1Of diseases,Th2-mediated
R277 T330 T331 arg1Of diseases,","
R278 T330 T333 arg1Of diseases,as
R279 T333 T332 arg1Of as,such
R280 T334 T333 arg2Of allergy,as
R770 T1013 T1011 arg1Of responses,Effective
R771 T1013 T1012 arg1Of responses,immune
R772 T1013 T1014 arg1Of responses,are
R773 T1013 T1015 arg2Of responses,characterized
R774 T1015 T1014 arg2Of characterized,are
R775 T1019 T1015 arg1Of activation,characterized
R776 T1019 T1016 arg2Of activation,by
R777 T1019 T1017 arg1Of activation,T
R778 T1019 T1018 arg1Of activation,cell
R779 T1019 T1020 arg1Of activation,","
R780 T1019 T1021 arg1Of activation,which
R781 T1019 T1022 arg1Of activation,directs
R782 T1022 T1028 modOf directs,to
R783 T1023 T1024 arg1Of adaptive,and
R784 T1025 T1024 arg2Of innate,and
R785 T1027 T1022 arg2Of responses,directs
R786 T1027 T1023 arg1Of responses,adaptive
R787 T1027 T1025 arg1Of responses,innate
R788 T1027 T1026 arg1Of responses,immune
R789 T1029 T1028 arg1Of kill,to
R790 T1029 T1031 arg1Of kill,efficiently
R791 T1030 T1029 arg2Of pathogens,kill
R792 T1032 T1033 arg1Of Dependent,on
R793 T1035 T1033 arg2Of pathogen,on
R794 T1035 T1034 arg1Of pathogen,the
R795 T1038 T1032 arg1Of cells,Dependent
R796 T1038 T1036 arg1Of cells,","
R797 T1038 T1037 arg1Of cells,T
R798 T1038 T1039 arg1Of cells,differentiate
R799 T1039 T1040 arg1Of differentiate,into
R800 T1042 T1040 arg2Of subtypes,into
R801 T1042 T1041 arg1Of subtypes,different
R802 T1042 T1043 arg1Of subtypes,","
R803 T1042 T1045 arg1Of subtypes,as
R804 T1045 T1044 arg1Of as,such
R805 T1046 T1047 arg1Of Th1,or
R806 T1048 T1047 arg2Of Th2,or
R807 T1049 T1045 arg2Of cells,as
R808 T1049 T1046 arg1Of cells,Th1
R809 T1049 T1048 arg1Of cells,Th2
R810 T1049 T1050 arg1Of cells,","
R811 T1049 T1051 arg1Of cells,which
R812 T1049 T1052 arg1Of cells,are
R813 T1049 T1054 arg1Of cells,efficient
R814 T1049 T1056 arg1Of cells,defeating
R815 T1052 T1055 arg1Of are,in
R816 T1054 T1052 arg2Of efficient,are
R817 T1054 T1053 arg1Of efficient,most
R818 T1056 T1055 arg2Of defeating,in
R819 T1056 T1061 arg1Of defeating,respectively
R820 T1057 T1058 arg1Of microbial,or
R821 T1059 T1058 arg2Of parasitic,or
R822 T1060 T1056 arg2Of invaders,defeating
R823 T1060 T1057 arg1Of invaders,microbial
R824 T1060 T1059 arg1Of invaders,parasitic
R825 T1063 T1062 arg1Of hallmark,A
R826 T1063 T1064 arg1Of hallmark,of
R827 T1063 T1070 arg1Of hallmark,is
R828 T1065 T1066 arg1Of Th1,and
R829 T1067 T1066 arg2Of Th2,and
R830 T1069 T1064 arg2Of pathways,of
R831 T1069 T1065 arg1Of pathways,Th1
R832 T1069 T1067 arg1Of pathways,Th2
R833 T1069 T1068 arg1Of pathways,differentiation
R834 T1072 T1070 arg2Of exclusiveness,is
R835 T1072 T1071 arg1Of exclusiveness,the
R836 T1072 T1073 arg1Of exclusiveness,of
R837 T1076 T1073 arg2Of phenotype,of
R838 T1076 T1074 arg1Of phenotype,the
R839 T1076 T1075 arg1Of phenotype,individual
R840 T1076 T1077 arg1Of phenotype,leading
R841 T1077 T1078 arg1Of leading,to
R842 T1077 T1086 arg1Of leading,to
R843 T1078 T1084 arg1Of to,but
R844 T1080 T1081 arg1Of Th1,or
R845 T1081 T1078 arg2Of or,to
R846 T1081 T1079 arg1Of or,either
R847 T1082 T1081 arg2Of Th2,or
R848 T1084 T1083 arg1Of but,","
R849 T1086 T1084 arg2Of to,but
R850 T1086 T1085 arg1Of to,not
R851 T1088 T1086 arg2Of populations,to
R852 T1088 T1087 arg1Of populations,mixed
R853 T1090 T1089 arg1Of exclusiveness,The
R854 T1090 T1091 arg1Of exclusiveness,of
R855 T1090 T1094 arg1Of exclusiveness,is
R856 T1090 T1095 arg2Of exclusiveness,provided
R857 T1093 T1091 arg2Of mechanism,of
R858 T1093 T1092 arg1Of mechanism,this
R859 T1095 T1094 arg2Of provided,is
R860 T1099 T1095 arg1Of process,provided
R861 T1099 T1096 arg2Of process,by
R862 T1099 T1097 arg1Of process,a
R863 T1099 T1098 arg1Of process,polarization
R864 T1099 T1100 arg1Of process,","
R865 T1099 T1101 arg1Of process,where
R866 T1103 T1102 arg1Of differentiation,Th2
R867 T1103 T1104 arg1Of differentiation,inhibits
R868 T1104 T1101 arg2Of inhibits,where
R869 T1106 T1105 arg1Of commitment,Th1
R870 T1106 T1107 arg1Of commitment,and
R871 T1107 T1104 arg2Of and,inhibits
R872 T1109 T1107 arg2Of versa,and
R873 T1109 T1108 arg1Of versa,vice
R874 T1111 T1112 arg1Of interleukin,4
R875 T1111 T1113 arg1Of interleukin,(
R876 T1111 T1116 arg1Of interleukin,–induced
R877 T1114 T1113 arg2Of IL-4,(
R878 T1115 T1113 arg3Of ),(
R879 T1116 T1110 arg1Of –induced,Specifically
R880 T1117 T1118 arg1Of STAT6,and
R881 T1118 T1120 arg1Of and,inhibit
R882 T1119 T1118 arg2Of GATA3,and
R883 T1120 T1116 arg2Of inhibit,–induced
R884 T1120 T1122 arg1Of inhibit,into
R885 T1120 T1125 arg1Of inhibit,in
R886 T1121 T1120 arg2Of differentiation,inhibit
R887 T1124 T1122 arg2Of cells,into
R888 T1124 T1123 arg1Of cells,Th1
R889 T1128 T1125 arg2Of phase,in
R890 T1128 T1126 arg1Of phase,the
R891 T1128 T1127 arg1Of phase,early
R892 T1128 T1129 arg1Of phase,of
R893 T1130 T1129 arg2Of commitment,of
R894 T1130 T1131 arg1Of commitment,[
R895 T1132 T1131 arg2Of "1,2",[
R896 T1133 T1131 arg3Of ],[
R897 T1134 T1135 arg1Of GATA3,is
R898 T1134 T1136 arg1Of GATA3,sufficient
R899 T1134 T1138 arg1Of GATA3,induce
R900 T1134 T1146 arg1Of GATA3,acts
R901 T1135 T1145 arg1Of is,and
R902 T1136 T1135 arg2Of sufficient,is
R903 T1138 T1136 arg2Of induce,sufficient
R904 T1138 T1137 arg1Of induce,to
R905 T1138 T1142 arg1Of induce,[
R906 T1141 T1138 arg2Of phenotype,induce
R907 T1141 T1139 arg1Of phenotype,a
R908 T1141 T1140 arg1Of phenotype,Th2
R909 T1143 T1142 arg2Of 3,[
R910 T1144 T1142 arg3Of ],[
R911 T1146 T1145 arg2Of acts,and
R912 T1146 T1149 arg1Of acts,through
R913 T1146 T1165 arg1Of acts,through
R914 T1148 T1147 arg1Of only,not
R915 T1149 T1163 arg1Of through,but
R916 T1151 T1149 arg2Of induction,through
R917 T1151 T1150 arg1Of induction,the
R918 T1151 T1152 arg1Of induction,of
R919 T1153 T1154 arg1Of IL-4,","
R920 T1154 T1156 arg1Of ",",and
R921 T1155 T1154 arg2Of IL-5,","
R922 T1156 T1152 arg2Of and,of
R923 T1156 T1158 arg1Of and,","
R924 T1157 T1156 arg2Of IL-13,and
R925 T1161 T1158 arg2Of cytokines,","
R926 T1161 T1159 arg1Of cytokines,the
R927 T1161 T1160 arg1Of cytokines,Th2
R928 T1163 T1148 arg1Of but,only
R929 T1163 T1162 arg1Of but,","
R930 T1163 T1164 arg1Of but,also
R931 T1165 T1163 arg2Of through,but
R932 T1167 T1165 arg2Of inhibition,through
R933 T1167 T1166 arg1Of inhibition,the
R934 T1167 T1168 arg1Of inhibition,of
R935 T1171 T1168 arg2Of factors,of
R936 T1171 T1169 arg1Of factors,Th1
R937 T1171 T1170 arg1Of factors,cell-specific
R938 T1171 T1172 arg1Of factors,[
R939 T1173 T1172 arg2Of 3,[
R940 T1174 T1172 arg3Of ],[
R941 T1177 T1178 arg1Of it,was
R942 T1177 T1179 arg2Of it,shown
R943 T1179 T1175 arg1Of shown,Recently
R944 T1179 T1176 arg1Of shown,","
R945 T1179 T1178 arg2Of shown,was
R946 T1181 T1183 arg1Of T-bet,modulates
R947 T1183 T1179 arg3Of modulates,shown
R948 T1183 T1180 arg1Of modulates,that
R949 T1183 T1182 arg1Of modulates,directly
R950 T1183 T1186 arg1Of modulates,","
R951 T1183 T1187 modOf modulates,suggesting
R952 T1185 T1183 arg2Of function,modulates
R953 T1185 T1184 arg1Of function,GATA3
R954 T1190 T1189 arg1Of factors,transcription
R955 T1190 T1191 arg1Of factors,compete
R956 T1190 T1202 arg1Of factors,integrating
R957 T1191 T1187 arg2Of compete,suggesting
R958 T1191 T1188 arg1Of compete,that
R959 T1191 T1192 arg1Of compete,in
R960 T1191 T1200 arg1Of compete,","
R961 T1191 T1202 modOf compete,integrating
R962 T1196 T1192 arg2Of phase,in
R963 T1196 T1193 arg1Of phase,the
R964 T1196 T1194 arg1Of phase,early
R965 T1196 T1195 arg1Of phase,differentiation
R966 T1196 T1197 arg1Of phase,of
R967 T1199 T1197 arg2Of cells,of
R968 T1199 T1198 arg1Of cells,T
R969 T1202 T1201 arg1Of integrating,potentially
R970 T1202 T1205 modOf integrating,to
R971 T1204 T1202 arg2Of signals,integrating
R972 T1204 T1203 arg1Of signals,environmental
R973 T1207 T1205 arg1Of imprint,to
R974 T1207 T1206 arg1Of imprint,finally
R975 T1207 T1212 arg1Of imprint,[
R976 T1211 T1207 arg2Of phenotype,imprint
R977 T1211 T1208 arg1Of phenotype,the
R978 T1211 T1209 arg1Of phenotype,T
R979 T1211 T1210 arg1Of phenotype,cell
R980 T1213 T1212 arg2Of "4,5",[
R981 T1214 T1212 arg3Of ],[
R982 T1218 T1215 arg1Of response,A
R983 T1218 T1216 arg1Of response,GATA3-dominated
R984 T1218 T1217 arg1Of response,immune
R985 T1218 T1219 arg1Of response,has
R986 T1218 T1220 arg1Of response,been
R987 T1218 T1221 arg2Of response,shown
R988 T1218 T1223 arg1Of response,be
R989 T1218 T1224 arg1Of response,essential
R990 T1221 T1219 arg2Of shown,has
R991 T1221 T1220 arg2Of shown,been
R992 T1221 T1231 arg1Of shown,and
R993 T1223 T1221 arg3Of be,shown
R994 T1223 T1222 arg1Of be,to
R995 T1223 T1228 arg1Of be,[
R996 T1224 T1223 arg2Of essential,be
R997 T1224 T1225 arg1Of essential,in
R998 T1227 T1225 arg2Of hyperresonsiveness,in
R999 T1227 T1226 arg1Of hyperresonsiveness,airway
R1000 T1229 T1228 arg2Of 6,[
R1001 T1230 T1228 arg3Of ],[
R1002 T1233 T1232 arg1Of responses,IL-4–dominated
R1003 T1233 T1234 arg1Of responses,can
R1004 T1233 T1235 arg1Of responses,break
R1005 T1235 T1231 arg2Of break,and
R1006 T1235 T1234 arg2Of break,can
R1007 T1238 T1235 arg2Of tolerance,break
R1008 T1238 T1236 arg1Of tolerance,antigen-specific
R1009 T1238 T1237 arg1Of tolerance,immune
R1010 T1238 T1239 arg1Of tolerance,[
R1011 T1240 T1239 arg2Of 7,[
R1012 T1241 T1239 arg3Of ],[
R1013 T1242 T1243 arg1Of Overexpression,of
R1014 T1242 T1262 arg1Of Overexpression,decreased
R1015 T1247 T1243 arg2Of form,of
R1016 T1247 T1244 arg1Of form,a
R1017 T1247 T1245 arg1Of form,dominant
R1018 T1247 T1246 arg1Of form,negative
R1019 T1247 T1248 arg1Of form,of
R1020 T1249 T1250 arg1Of GATA3,[
R1021 T1249 T1253 arg1Of GATA3,or
R1022 T1251 T1250 arg2Of 8,[
R1023 T1252 T1250 arg3Of ],[
R1024 T1253 T1248 arg2Of or,of
R1025 T1253 T1255 arg1Of or,with
R1026 T1254 T1253 arg2Of treatment,or
R1027 T1258 T1255 arg2Of blockade,with
R1028 T1258 T1256 arg1Of blockade,antisense-mediated
R1029 T1258 T1257 arg1Of blockade,GATA3
R1030 T1258 T1259 arg1Of blockade,[
R1031 T1260 T1259 arg2Of 9,[
R1032 T1261 T1259 arg3Of ],[
R1033 T1264 T1262 arg2Of severity,decreased
R1034 T1264 T1263 arg1Of severity,the
R1035 T1264 T1265 arg1Of severity,of
R1036 T1269 T1265 arg2Of hyper-responsiveness,of
R1037 T1269 T1266 arg1Of hyper-responsiveness,the
R1038 T1269 T1267 arg1Of hyper-responsiveness,allergic
R1039 T1269 T1268 arg1Of hyper-responsiveness,airway
R1040 T1271 T1270 arg1Of discovery,The
R1041 T1271 T1272 arg1Of discovery,of
R1042 T1271 T1279 arg1Of discovery,highlights
R1043 T1276 T1275 arg2Of Treg,(
R1044 T1277 T1275 arg3Of ),(
R1045 T1278 T1272 arg2Of cells,of
R1046 T1278 T1273 arg1Of cells,regulatory
R1047 T1278 T1274 arg1Of cells,T
R1048 T1278 T1275 arg1Of cells,(
R1049 T1281 T1279 arg2Of phenotype,highlights
R1050 T1281 T1280 arg1Of phenotype,another
R1051 T1281 T1282 arg1Of phenotype,of
R1052 T1281 T1285 arg1Of phenotype,","
R1053 T1281 T1286 arg1Of phenotype,which
R1054 T1281 T1287 arg1Of phenotype,is
R1055 T1281 T1288 arg1Of phenotype,essential
R1056 T1284 T1282 arg2Of cells,of
R1057 T1284 T1283 arg1Of cells,T
R1058 T1288 T1287 arg2Of essential,is
R1059 T1288 T1289 arg1Of essential,for
R1060 T1290 T1289 arg2Of tolerance,for
R1061 T1290 T1291 arg1Of tolerance,against
R1062 T1292 T1291 arg2Of self-antigens,against
R1063 T1294 T1293 arg1Of occurring,Naturally
R1064 T1294 T1295 arg1Of occurring,","
R1065 T1296 T1295 arg2Of thymus-derived,","
R1066 T1299 T1298 arg2Of nTreg,(
R1067 T1300 T1298 arg3Of ),(
R1068 T1301 T1294 arg1Of cells,occurring
R1069 T1301 T1296 arg1Of cells,thymus-derived
R1070 T1301 T1297 arg1Of cells,Treg
R1071 T1301 T1298 arg1Of cells,(
R1072 T1301 T1302 arg1Of cells,are
R1073 T1301 T1303 arg2Of cells,generated
R1074 T1301 T1308 arg1Of cells,are
R1075 T1301 T1309 arg2Of cells,assumed
R1076 T1301 T1311 arg1Of cells,protect
R1077 T1303 T1302 arg2Of generated,are
R1078 T1303 T1304 arg1Of generated,in
R1079 T1303 T1307 arg1Of generated,and
R1080 T1306 T1304 arg2Of thymus,in
R1081 T1306 T1305 arg1Of thymus,the
R1082 T1309 T1307 arg2Of assumed,and
R1083 T1309 T1308 arg2Of assumed,are
R1084 T1311 T1309 arg3Of protect,assumed
R1085 T1311 T1310 arg1Of protect,to
R1086 T1311 T1312 arg1Of protect,against
R1087 T1314 T1312 arg2Of activity,against
R1088 T1314 T1313 arg1Of activity,the
R1089 T1314 T1315 arg1Of activity,of
R1090 T1314 T1319 arg1Of activity,in
R1091 T1318 T1315 arg2Of cells,of
R1092 T1318 T1316 arg1Of cells,autoreactive
R1093 T1318 T1317 arg1Of cells,T
R1094 T1321 T1319 arg2Of periphery,in
R1095 T1321 T1320 arg1Of periphery,the
R1096 T1323 T1322 arg1Of cells,These
R1097 T1323 T1324 arg1Of cells,express
R1098 T1323 T1332 arg1Of cells,express
R1099 T1324 T1330 arg1Of express,and
R1100 T1329 T1324 arg2Of FOXP3,express
R1101 T1329 T1325 arg1Of FOXP3,the
R1102 T1329 T1326 arg1Of FOXP3,forkhead
R1103 T1329 T1327 arg1Of FOXP3,transcription
R1104 T1329 T1328 arg1Of FOXP3,factor
R1105 T1330 T1338 arg1Of and,but
R1106 T1332 T1330 arg2Of express,and
R1107 T1332 T1331 arg1Of express,constitutively
R1108 T1332 T1334 arg1Of express,on
R1109 T1333 T1332 arg2Of CD25,express
R1110 T1336 T1334 arg2Of surface,on
R1111 T1336 T1335 arg1Of surface,their
R1112 T1338 T1337 arg1Of but,","
R1113 T1339 T1340 arg1Of they,lack
R1114 T1340 T1338 arg2Of lack,but
R1115 T1341 T1340 arg2Of expression,lack
R1116 T1341 T1342 arg1Of expression,of
R1117 T1343 T1344 arg1Of Th1,or
R1118 T1345 T1344 arg2Of Th2,or
R1119 T1346 T1342 arg2Of cytokines,of
R1120 T1346 T1343 arg1Of cytokines,Th1
R1121 T1346 T1345 arg1Of cytokines,Th2
R1122 T1348 T1347 arg1Of interesting,Particularly
R1123 T1348 T1349 arg2Of interesting,are
R1124 T1352 T1348 arg1Of cells,interesting
R1125 T1352 T1349 arg1Of cells,are
R1126 T1352 T1350 arg1Of cells,those
R1127 T1352 T1351 arg1Of cells,Treg
R1128 T1352 T1353 arg1Of cells,that
R1129 T1352 T1354 arg1Of cells,are
R1130 T1352 T1355 arg2Of cells,generated
R1131 T1352 T1361 arg1Of cells,are
R1132 T1355 T1354 arg2Of generated,are
R1133 T1355 T1356 arg1Of generated,in
R1134 T1355 T1359 arg1Of generated,and
R1135 T1358 T1356 arg2Of periphery,in
R1136 T1358 T1357 arg1Of periphery,the
R1137 T1361 T1359 arg2Of are,and
R1138 T1361 T1360 arg1Of are,thus
R1139 T1363 T1361 arg2Of targets,are
R1140 T1363 T1362 arg1Of targets,potential
R1141 T1363 T1364 arg1Of targets,for
R1142 T1366 T1364 arg2Of interventions,for
R1143 T1366 T1365 arg1Of interventions,therapeutic
R1144 T1371 T1370 arg2Of iTreg,(
R1145 T1372 T1370 arg3Of ),(
R1146 T1373 T1367 arg1Of cells,These
R1147 T1373 T1368 arg2Of cells,induced
R1148 T1373 T1369 arg1Of cells,Treg
R1149 T1373 T1370 arg1Of cells,(
R1150 T1373 T1374 arg1Of cells,were
R1151 T1373 T1375 arg2Of cells,reported
R1152 T1373 T1377 arg1Of cells,express
R1153 T1375 T1374 arg2Of reported,were
R1154 T1377 T1375 arg3Of express,reported
R1155 T1377 T1376 arg1Of express,to
R1156 T1377 T1379 arg1Of express,[
R1157 T1378 T1377 arg2Of FOXP3,express
R1158 T1380 T1379 arg2Of 10,[
R1159 T1381 T1379 arg3Of ],[
R1160 T1384 T1382 arg1Of mechanisms,The
R1161 T1384 T1383 arg1Of mechanisms,exact
R1162 T1384 T1385 arg1Of mechanisms,of
R1163 T1384 T1388 arg1Of mechanisms,are
R1164 T1384 T1389 arg1Of mechanisms,unclear
R1165 T1387 T1385 arg2Of generation,of
R1166 T1387 T1386 arg1Of generation,iTreg
R1167 T1388 T1391 arg1Of are,but
R1168 T1389 T1388 arg2Of unclear,are
R1169 T1391 T1390 arg1Of but,","
R1170 T1396 T1395 arg2Of TCR,(
R1171 T1397 T1395 arg3Of ),(
R1172 T1398 T1392 arg1Of triggering,T
R1173 T1398 T1393 arg1Of triggering,cell
R1174 T1398 T1394 arg1Of triggering,receptor
R1175 T1398 T1395 arg1Of triggering,(
R1176 T1398 T1399 arg1Of triggering,has
R1177 T1398 T1400 arg1Of triggering,been
R1178 T1398 T1401 arg2Of triggering,shown
R1179 T1398 T1403 arg1Of triggering,induce
R1180 T1401 T1399 arg2Of shown,has
R1181 T1401 T1400 arg2Of shown,been
R1182 T1401 T1414 arg1Of shown,","
R1183 T1403 T1401 arg3Of induce,shown
R1184 T1403 T1402 arg1Of induce,to
R1185 T1405 T1404 arg1Of expression,FOXP3
R1186 T1405 T1406 arg1Of expression,and
R1187 T1406 T1403 arg2Of and,induce
R1188 T1406 T1409 arg1Of and,in
R1189 T1408 T1406 arg2Of cells,and
R1190 T1408 T1407 arg1Of cells,suppressive
R1191 T1410 T1409 arg2Of human,in
R1192 T1410 T1411 arg1Of human,[
R1193 T1412 T1411 arg2Of "11,12",[
R1194 T1413 T1411 arg3Of ],[
R1195 T1414 T1391 arg2Of ",",but
R1196 T1417 T1416 arg1Of phenotype,the
R1197 T1417 T1418 arg1Of phenotype,appears
R1198 T1417 T1420 arg1Of phenotype,be
R1199 T1417 T1421 arg1Of phenotype,of
R1200 T1418 T1414 arg2Of appears,","
R1201 T1418 T1415 arg1Of appears,however
R1202 T1420 T1418 arg2Of be,appears
R1203 T1420 T1419 arg1Of be,to
R1204 T1421 T1420 arg2Of of,be
R1205 T1423 T1421 arg2Of nature,of
R1206 T1423 T1422 arg1Of nature,transient
R1207 T1423 T1424 arg1Of nature,[
R1208 T1425 T1424 arg2Of "13,14",[
R1209 T1426 T1424 arg3Of ],[
R1210 T1427 T1428 arg1Of TGF-β,has
R1211 T1427 T1429 arg1Of TGF-β,been
R1212 T1427 T1430 arg2Of TGF-β,demonstrated
R1213 T1427 T1432 arg1Of TGF-β,be
R1214 T1427 T1433 arg1Of TGF-β,important
R1215 T1430 T1428 arg2Of demonstrated,has
R1216 T1430 T1429 arg2Of demonstrated,been
R1217 T1432 T1430 arg3Of be,demonstrated
R1218 T1432 T1431 arg1Of be,to
R1219 T1432 T1442 arg1Of be,vitro
R1220 T1432 T1445 arg1Of be,vivo
R1221 T1432 T1446 arg1Of be,","
R1222 T1432 T1447 arg1Of be,since
R1223 T1433 T1432 arg2Of important,be
R1224 T1433 T1434 arg1Of important,for
R1225 T1437 T1434 arg2Of induction,for
R1226 T1437 T1435 arg1Of induction,the
R1227 T1437 T1436 arg1Of induction,persistent
R1228 T1437 T1438 arg1Of induction,of
R1229 T1440 T1438 arg2Of cells,of
R1230 T1440 T1439 arg1Of cells,these
R1231 T1442 T1441 arg1Of vitro,in
R1232 T1442 T1443 arg1Of vitro,and
R1233 T1445 T1443 arg2Of vivo,and
R1234 T1445 T1444 arg1Of vivo,in
R1235 T1448 T1449 arg1Of animals,lacking
R1236 T1448 T1455 arg1Of animals,have
R1237 T1448 T1464 arg1Of animals,suffer
R1238 T1451 T1449 arg2Of TGF-βRII,lacking
R1239 T1451 T1450 arg1Of TGF-βRII,the
R1240 T1451 T1452 arg1Of TGF-βRII,on
R1241 T1454 T1452 arg2Of cells,on
R1242 T1454 T1453 arg1Of cells,T
R1243 T1455 T1460 arg1Of have,[
R1244 T1455 T1463 arg1Of have,and
R1245 T1459 T1455 arg2Of cells,have
R1246 T1459 T1456 arg1Of cells,fewer
R1247 T1459 T1457 arg1Of cells,peripherally
R1248 T1459 T1458 arg1Of cells,iTreg
R1249 T1461 T1460 arg2Of 15,[
R1250 T1462 T1460 arg3Of ],[
R1251 T1463 T1447 arg2Of and,since
R1252 T1463 T1472 arg1Of and,[
R1253 T1464 T1463 arg2Of suffer,and
R1254 T1464 T1465 arg1Of suffer,from
R1255 T1471 T1465 arg2Of disease,from
R1256 T1471 T1466 arg1Of disease,a
R1257 T1471 T1467 arg1Of disease,T
R1258 T1471 T1468 arg1Of disease,cell–dependent
R1259 T1471 T1469 arg1Of disease,multiorgan
R1260 T1471 T1470 arg1Of disease,inflammatory
R1261 T1473 T1472 arg2Of 16,[
R1262 T1474 T1472 arg3Of ],[
R1263 T1477 T1476 arg1Of effect,the
R1264 T1477 T1478 arg1Of effect,of
R1265 T1477 T1480 arg1Of effect,on
R1266 T1477 T1487 arg1Of effect,has
R1267 T1477 T1488 arg1Of effect,been
R1268 T1477 T1489 arg2Of effect,demonstrated
R1269 T1479 T1478 arg2Of TGF-β,of
R1270 T1481 T1482 arg1Of natural,and
R1271 T1483 T1482 arg2Of inducible,and
R1272 T1486 T1480 arg2Of induction,on
R1273 T1486 T1481 arg1Of induction,natural
R1274 T1486 T1483 arg1Of induction,inducible
R1275 T1486 T1484 arg1Of induction,Treg
R1276 T1486 T1485 arg1Of induction,cell
R1277 T1489 T1475 arg2Of demonstrated,Although
R1278 T1489 T1487 arg2Of demonstrated,has
R1279 T1489 T1488 arg2Of demonstrated,been
R1280 T1489 T1490 arg1Of demonstrated,repeatedly
R1281 T1489 T1491 arg1Of demonstrated,[
R1282 T1492 T1491 arg2Of "15,17",[
R1283 T1493 T1491 arg3Of ],[
R1284 T1497 T1495 arg1Of mechanisms,its
R1285 T1497 T1496 arg1Of mechanisms,molecular
R1286 T1497 T1498 arg1Of mechanisms,remain
R1287 T1497 T1500 arg1Of mechanisms,be
R1288 T1497 T1501 arg2Of mechanisms,identified
R1289 T1498 T1475 arg1Of remain,Although
R1290 T1498 T1494 arg1Of remain,","
R1291 T1501 T1498 arg2Of identified,remain
R1292 T1501 T1499 arg1Of identified,to
R1293 T1501 T1500 arg2Of identified,be
R1294 T1504 T1502 arg1Of study,The
R1295 T1504 T1503 arg1Of study,current
R1296 T1504 T1505 arg1Of study,provides
R1297 T1506 T1505 arg2Of evidence,provides
R1298 T1508 T1509 arg1Of GATA3,and
R1299 T1509 T1511 arg1Of and,play
R1300 T1510 T1509 arg2Of FOXP3,and
R1301 T1511 T1506 arg2Of play,evidence
R1302 T1511 T1507 arg1Of play,that
R1303 T1511 T1515 arg1Of play,in
R1304 T1511 T1528 arg1Of play,","
R1305 T1511 T1529 arg1Of play,respectively
R1306 T1514 T1511 arg2Of role,play
R1307 T1514 T1512 arg1Of role,a
R1308 T1514 T1513 arg1Of role,competitive
R1309 T1518 T1515 arg2Of commitment,in
R1310 T1518 T1516 arg1Of commitment,iTreg
R1311 T1518 T1517 arg1Of commitment,cell
R1312 T1518 T1519 arg1Of commitment,as
R1313 T1520 T1521 arg1Of T-bet,and
R1314 T1521 T1519 arg2Of and,as
R1315 T1521 T1523 arg1Of and,for
R1316 T1522 T1521 arg2Of GATA3,and
R1317 T1524 T1525 arg1Of Th1,and
R1318 T1526 T1525 arg2Of Th2,and
R1319 T1527 T1523 arg2Of differentiation,for
R1320 T1527 T1524 arg1Of differentiation,Th1
R1321 T1527 T1526 arg1Of differentiation,Th2
R1322 T1530 T1531 arg1Of We,show
R1323 T1533 T1534 arg1Of GATA3,inhibits
R1324 T1534 T1532 arg1Of inhibits,that
R1325 T1534 T1537 arg1Of inhibits,and
R1326 T1536 T1534 arg2Of induction,inhibits
R1327 T1536 T1535 arg1Of induction,FOXP3
R1328 T1537 T1531 arg2Of and,show
R1329 T1538 T1537 arg2Of that,and
R1330 T1539 T1540 arg1Of IL-4,limits
R1331 T1540 T1538 arg2Of limits,that
R1332 T1540 T1543 arg1Of limits,in
R1333 T1540 T1547 arg1Of limits,","
R1334 T1540 T1550 arg1Of limits,vitro
R1335 T1540 T1553 arg1Of limits,vivo
R1336 T1542 T1540 arg2Of expression,limits
R1337 T1542 T1541 arg1Of expression,FOXP3
R1338 T1546 T1543 arg2Of way,in
R1339 T1546 T1544 arg1Of way,a
R1340 T1546 T1545 arg1Of way,GATA3-mediated
R1341 T1550 T1549 arg1Of vitro,in
R1342 T1550 T1551 arg1Of vitro,and
R1343 T1551 T1548 arg1Of and,both
R1344 T1553 T1551 arg2Of vivo,and
R1345 T1553 T1552 arg1Of vivo,in
R1346 T1554 T1556 arg1Of We,show
R1347 T1556 T1555 arg1Of show,also
R1348 T1558 T1560 arg1Of GATA3,binds
R1349 T1558 T1567 arg1Of GATA3,prevents
R1350 T1560 T1561 arg1Of binds,to
R1351 T1560 T1565 arg1Of binds,and
R1352 T1564 T1561 arg2Of promoter,to
R1353 T1564 T1562 arg1Of promoter,the
R1354 T1564 T1563 arg1Of promoter,FOXP3
R1355 T1565 T1556 arg2Of and,show
R1356 T1565 T1557 arg1Of and,that
R1357 T1565 T1559 arg1Of and,directly
R1358 T1567 T1565 arg2Of prevents,and
R1359 T1567 T1566 arg1Of prevents,thereby
R1360 T1567 T1573 arg1Of prevents,","
R1361 T1569 T1567 arg2Of induction,prevents
R1362 T1569 T1568 arg1Of induction,the
R1363 T1569 T1570 arg1Of induction,of
R1364 T1572 T1570 arg2Of gene,of
R1365 T1572 T1571 arg1Of gene,this
R1366 T1574 T1567 arg3Of demonstrating,prevents
R1367 T1577 T1576 arg1Of differentiation,Th2
R1368 T1577 T1578 arg1Of differentiation,overrules
R1369 T1578 T1574 arg2Of overrules,demonstrating
R1370 T1578 T1575 arg1Of overrules,that
R1371 T1580 T1578 arg2Of induction,overrules
R1372 T1580 T1579 arg1Of induction,iTreg
R2365 T3017 T3016 arg1Of Induction,FOXP3
R2366 T3017 T3018 arg1Of Induction,in
R2367 T3021 T3018 arg2Of Subsets,in
R2368 T3021 T3019 arg1Of Subsets,T
R2369 T3021 T3020 arg1Of Subsets,Cell
R2370 T3022 T3023 arg1Of It,is
R2371 T3022 T3024 arg2Of It,assumed
R2372 T3024 T3023 arg2Of assumed,is
R2373 T3027 T3026 arg1Of expression,FOXP3
R2374 T3027 T3028 arg1Of expression,can
R2375 T3027 T3029 arg1Of expression,be
R2376 T3027 T3030 arg2Of expression,induced
R2377 T3030 T3024 arg3Of induced,assumed
R2378 T3030 T3025 arg1Of induced,that
R2379 T3030 T3028 arg2Of induced,can
R2380 T3030 T3029 arg2Of induced,be
R2381 T3030 T3031 arg1Of induced,in
R2382 T3034 T3031 arg2Of cells,in
R2383 T3034 T3032 arg1Of cells,nonregulatory
R2384 T3034 T3033 arg1Of cells,T
R2385 T3034 T3035 arg1Of cells,","
R2386 T3034 T3036 arg1Of cells,which
R2387 T3034 T3037 arg1Of cells,is
R2388 T3040 T3037 arg2Of step,is
R2389 T3040 T3038 arg1Of step,an
R2390 T3040 T3039 arg1Of step,important
R2391 T3040 T3041 arg1Of step,in
R2392 T3044 T3041 arg2Of differentiation,in
R2393 T3044 T3042 arg1Of differentiation,iTreg
R2394 T3044 T3043 arg1Of differentiation,cell
R2395 T3047 T3048 arg1Of it,is
R2396 T3047 T3050 arg2Of it,known
R2397 T3050 T3045 arg1Of known,However
R2398 T3050 T3046 arg1Of known,","
R2399 T3050 T3048 arg2Of known,is
R2400 T3050 T3049 arg1Of known,not
R2401 T3050 T3051 arg1Of known,if
R2402 T3055 T3052 arg1Of cells,all
R2403 T3055 T3053 arg1Of cells,CD4+
R2404 T3055 T3054 arg1Of cells,T
R2405 T3055 T3056 arg1Of cells,have
R2406 T3056 T3051 arg2Of have,if
R2407 T3059 T3056 arg2Of capacity,have
R2408 T3059 T3057 arg1Of capacity,the
R2409 T3059 T3058 arg1Of capacity,same
R2410 T3059 T3060 modOf capacity,to
R2411 T3061 T3060 arg1Of express,to
R2412 T3062 T3061 arg2Of FOXP3,express
R2413 T3064 T3063 arg1Of investigate,To
R2414 T3066 T3067 arg1Of FOXP3,can
R2415 T3066 T3068 arg1Of FOXP3,be
R2416 T3066 T3069 arg2Of FOXP3,expressed
R2417 T3069 T3064 arg2Of expressed,investigate
R2418 T3069 T3065 arg1Of expressed,whether
R2419 T3069 T3067 arg2Of expressed,can
R2420 T3069 T3068 arg2Of expressed,be
R2421 T3069 T3070 arg1Of expressed,by
R2422 T3069 T3076 arg1Of expressed,if
R2423 T3070 T3075 arg1Of by,or
R2424 T3074 T3070 arg2Of subset,by
R2425 T3074 T3071 arg1Of subset,any
R2426 T3074 T3072 arg1Of subset,T
R2427 T3074 T3073 arg1Of subset,cell
R2428 T3076 T3075 arg2Of if,or
R2429 T3077 T3078 arg1Of expression,is
R2430 T3077 T3079 arg1Of expression,restricted
R2431 T3078 T3076 arg2Of is,if
R2432 T3079 T3078 arg2Of restricted,is
R2433 T3079 T3080 arg1Of restricted,to
R2434 T3083 T3080 arg2Of lineage,to
R2435 T3083 T3081 arg1Of lineage,a
R2436 T3083 T3082 arg1Of lineage,distinct
R2437 T3087 T3064 arg1Of expression,investigate
R2438 T3087 T3085 arg1Of expression,FOXP3
R2439 T3087 T3086 arg1Of expression,mRNA
R2440 T3087 T3088 arg1Of expression,was
R2441 T3087 T3089 arg2Of expression,analyzed
R2442 T3089 T3063 modOf analyzed,To
R2443 T3089 T3084 arg1Of analyzed,","
R2444 T3089 T3088 arg2Of analyzed,was
R2445 T3089 T3090 arg1Of analyzed,in
R2446 T3094 T3091 arg1Of cells,freshly
R2447 T3094 T3092 arg2Of cells,isolated
R2448 T3094 T3093 arg1Of cells,T
R2449 T3094 T3096 arg1Of cells,as
R2450 T3094 T3100 arg1Of cells,","
R2451 T3096 T3095 arg1Of as,such
R2452 T3099 T3096 arg2Of cells,as
R2453 T3099 T3097 arg1Of cells,CD25-depleted
R2454 T3099 T3098 arg1Of cells,CD4+
R2455 T3100 T3115 arg1Of ",",as
R2456 T3102 T3103 arg1Of naive,or
R2457 T3104 T3103 arg2Of CD45RO+,or
R2458 T3107 T3100 arg2Of cells,","
R2459 T3107 T3101 arg1Of cells,CD45RA+
R2460 T3107 T3102 arg1Of cells,naive
R2461 T3107 T3104 arg1Of cells,CD45RO+
R2462 T3107 T3105 arg1Of cells,memory
R2463 T3107 T3106 arg1Of cells,T
R2464 T3107 T3108 arg1Of cells,(
R2465 T3110 T3108 arg2Of 1A,(
R2466 T3110 T3109 arg1Of 1A,Figure
R2467 T3111 T3108 arg3Of ),(
R2468 T3115 T3090 arg2Of as,in
R2469 T3115 T3112 arg1Of as,","
R2470 T3115 T3113 arg1Of as,as
R2471 T3115 T3114 arg1Of as,well
R2472 T3117 T3115 arg2Of cells,as
R2473 T3117 T3116 arg1Of cells,T
R2474 T3117 T3118 arg2Of cells,driven
R2475 T3118 T3120 arg1Of driven,vitro
R2476 T3118 T3121 arg1Of driven,toward
R2477 T3120 T3119 arg1Of vitro,in
R2478 T3122 T3123 arg1Of Th1,","
R2479 T3123 T3126 arg1Of ",",or
R2480 T3124 T3123 arg2Of Th2,","
R2481 T3126 T3125 arg1Of or,","
R2482 T3127 T3126 arg2Of iTreg,or
R2483 T3128 T3121 arg2Of phenotypes,toward
R2484 T3128 T3122 arg1Of phenotypes,Th1
R2485 T3128 T3124 arg1Of phenotypes,Th2
R2486 T3128 T3127 arg1Of phenotypes,iTreg
R2487 T3128 T3129 arg1Of phenotypes,(
R2488 T3131 T3130 arg1Of 1B,Figure
R2489 T3131 T3132 arg1Of 1B,;
R2490 T3132 T3129 arg2Of ;,(
R2491 T3132 T3134 arg1Of ;,on
R2492 T3133 T3132 arg2Of phenotype,;
R2493 T3136 T3134 arg2Of S1,on
R2494 T3136 T3135 arg1Of S1,Figure
R2495 T3137 T3129 arg3Of ),(
R2496 T3139 T3138 arg1Of CD4+CD25–,The
R2497 T3139 T3140 arg1Of CD4+CD25–,","
R2498 T3139 T3142 arg1Of CD4+CD25–,","
R2499 T3141 T3140 arg2Of CD45RA+,","
R2500 T3142 T3145 arg1Of ",",and
R2501 T3143 T3142 arg2Of CD45RO+,","
R2502 T3145 T3144 arg1Of and,","
R2503 T3145 T3147 arg1Of and,were
R2504 T3145 T3148 arg1Of and,able
R2505 T3145 T3151 arg1Of and,induce
R2506 T3146 T3145 arg2Of CD4+CD45RO+CD25–,and
R2507 T3148 T3147 arg2Of able,were
R2508 T3151 T3148 arg2Of induce,able
R2509 T3151 T3149 arg1Of induce,to
R2510 T3151 T3150 arg1Of induce,significantly
R2511 T3151 T3157 arg1Of induce,upon
R2512 T3153 T3151 arg2Of mRNA,induce
R2513 T3153 T3152 arg1Of mRNA,FOXP3
R2514 T3154 T3155 arg1Of up,to
R2515 T3157 T3154 arg1Of upon,up
R2516 T3157 T3156 arg1Of upon,30-fold
R2517 T3159 T3158 arg1Of activation,TCR
R2518 T3159 T3160 arg1Of activation,and
R2519 T3160 T3157 arg2Of and,upon
R2520 T3161 T3160 arg2Of addition,and
R2521 T3161 T3162 arg1Of addition,of
R2522 T3163 T3162 arg2Of TGF-β,of
R2523 T3165 T3164 arg1Of cells,Th1
R2524 T3165 T3166 arg1Of cells,showed
R2525 T3170 T3166 arg2Of increase,showed
R2526 T3170 T3167 arg1Of increase,only
R2527 T3170 T3168 arg1Of increase,a
R2528 T3170 T3169 arg1Of increase,10-fold
R2529 T3172 T3171 arg2Of contrast,In
R2530 T3175 T3174 arg1Of cells,Th2
R2531 T3175 T3176 arg2Of cells,stimulated
R2532 T3175 T3181 arg1Of cells,did
R2533 T3175 T3183 arg1Of cells,increase
R2534 T3176 T3177 arg1Of stimulated,under
R2535 T3180 T3177 arg2Of conditions,under
R2536 T3180 T3178 arg1Of conditions,the
R2537 T3180 T3179 arg1Of conditions,same
R2538 T3183 T3171 arg1Of increase,In
R2539 T3183 T3173 arg1Of increase,","
R2540 T3183 T3181 arg2Of increase,did
R2541 T3183 T3182 arg1Of increase,not
R2542 T3185 T3183 arg2Of expression,increase
R2543 T3185 T3184 arg1Of expression,FOXP3
R2544 T3188 T3187 arg1Of vitro,in
R2545 T3189 T3188 arg1Of generated,vitro
R2546 T3191 T3186 arg1Of cells,The
R2547 T3191 T3189 arg1Of cells,generated
R2548 T3191 T3190 arg1Of cells,iTreg
R2549 T3191 T3192 arg1Of cells,were
R2550 T3191 T3193 arg1Of cells,unable
R2551 T3191 T3196 arg1Of cells,up-regulate
R2552 T3193 T3192 arg2Of unable,were
R2553 T3196 T3193 arg2Of up-regulate,unable
R2554 T3196 T3194 arg1Of up-regulate,to
R2555 T3196 T3195 arg1Of up-regulate,further
R2556 T3197 T3196 arg2Of FOXP3,up-regulate
R2557 T3197 T3198 arg1Of FOXP3,","
R2558 T3197 T3199 arg1Of FOXP3,which
R2559 T3197 T3200 arg1Of FOXP3,was
R2560 T3197 T3202 arg1Of FOXP3,at
R2561 T3200 T3201 arg1Of was,already
R2562 T3200 T3205 arg1Of was,under
R2563 T3202 T3200 arg2Of at,was
R2564 T3204 T3202 arg2Of levels,at
R2565 T3204 T3203 arg1Of levels,high
R2566 T3208 T3205 arg2Of conditions,under
R2567 T3208 T3206 arg1Of conditions,the
R2568 T3208 T3207 arg1Of conditions,resting
R2569 T3208 T3209 arg1Of conditions,(
R2570 T3211 T3209 arg2Of 1B,(
R2571 T3211 T3210 arg1Of 1B,Figure
R2572 T3211 T3212 arg1Of 1B,","
R2573 T3214 T3212 arg2Of panel,","
R2574 T3214 T3213 arg1Of panel,right
R2575 T3215 T3209 arg3Of ),(
R2576 T3217 T3216 arg1Of Cells,Th2
R2577 T3217 T3218 arg1Of Cells,are
R2578 T3217 T3219 arg2Of Cells,known
R2579 T3217 T3221 arg1Of Cells,produce
R2580 T3219 T3218 arg2Of known,are
R2581 T3221 T3219 arg3Of produce,known
R2582 T3221 T3220 arg1Of produce,to
R2583 T3221 T3223 arg1Of produce,upon
R2584 T3222 T3221 arg2Of IL-4,produce
R2585 T3224 T3223 arg2Of activation,upon
R2586 T3224 T3225 arg1Of activation,","
R2587 T3224 T3226 arg1Of activation,which
R2588 T3224 T3227 arg1Of activation,may
R2589 T3224 T3228 arg1Of activation,interact
R2590 T3224 T3234 arg1Of activation,prevent
R2591 T3228 T3227 arg2Of interact,may
R2592 T3228 T3229 arg1Of interact,with
R2593 T3228 T3232 arg1Of interact,and
R2594 T3231 T3229 arg2Of signaling,with
R2595 T3231 T3230 arg1Of signaling,TGF-β
R2596 T3234 T3232 arg2Of prevent,and
R2597 T3234 T3233 arg1Of prevent,thus
R2598 T3236 T3234 arg2Of induction,prevent
R2599 T3236 T3235 arg1Of induction,FOXP3
R2600 T3240 T3239 arg1Of neutralization,the
R2601 T3240 T3241 arg1Of neutralization,of
R2602 T3240 T3248 arg1Of neutralization,did
R2603 T3240 T3250 arg1Of neutralization,rescue
R2604 T3242 T3241 arg2Of IL-4,of
R2605 T3242 T3243 arg1Of IL-4,with
R2606 T3247 T3243 arg2Of antibody,with
R2607 T3247 T3244 arg1Of antibody,a
R2608 T3247 T3245 arg1Of antibody,blocking
R2609 T3247 T3246 arg1Of antibody,IL-4
R2610 T3250 T3237 arg1Of rescue,However
R2611 T3250 T3238 arg1Of rescue,","
R2612 T3250 T3248 arg2Of rescue,did
R2613 T3250 T3249 arg1Of rescue,not
R2614 T3250 T3253 arg1Of rescue,in
R2615 T3252 T3250 arg2Of expression,rescue
R2616 T3252 T3251 arg1Of expression,FOXP3
R2617 T3257 T3253 arg2Of cells,in
R2618 T3257 T3254 arg1Of cells,the
R2619 T3257 T3255 arg2Of cells,differentiated
R2620 T3257 T3256 arg1Of cells,Th2
R2621 T3257 T3258 arg1Of cells,(
R2622 T3260 T3258 arg2Of data,(
R2623 T3260 T3259 arg1Of data,unpublished
R2624 T3261 T3258 arg3Of ),(
R2625 T3263 T3262 arg1Of data,These
R2626 T3263 T3264 arg1Of data,demonstrated
R2627 T3267 T3266 arg1Of cells,Th2
R2628 T3267 T3268 arg1Of cells,have
R2629 T3268 T3264 arg2Of have,demonstrated
R2630 T3268 T3265 arg1Of have,that
R2631 T3271 T3268 arg2Of capacity,have
R2632 T3271 T3269 arg1Of capacity,a
R2633 T3271 T3270 arg2Of capacity,limited
R2634 T3271 T3272 modOf capacity,to
R2635 T3273 T3272 arg1Of express,to
R2636 T3274 T3273 arg2Of FOXP3,express
R2637 T3274 T3275 arg1Of FOXP3,(
R2638 T3277 T3275 arg2Of 1B,(
R2639 T3277 T3276 arg1Of 1B,Figure
R2640 T3278 T3275 arg3Of ),(
R2641 T3280 T3279 arg1Of inability,The
R2642 T3280 T3281 arg1Of inability,of
R2643 T3280 T3284 modOf inability,to
R2644 T3280 T3287 arg1Of inability,was
R2645 T3280 T3289 arg2Of inability,documented
R2646 T3283 T3281 arg2Of cells,of
R2647 T3283 T3282 arg1Of cells,Th2
R2648 T3285 T3284 arg1Of express,to
R2649 T3286 T3285 arg2Of FOXP3,express
R2650 T3289 T3287 arg2Of documented,was
R2651 T3289 T3288 arg1Of documented,also
R2652 T3289 T3290 arg1Of documented,at
R2653 T3289 T3294 arg1Of documented,","
R2654 T3289 T3295 modOf documented,confirming
R2655 T3293 T3290 arg2Of level,at
R2656 T3293 T3291 arg1Of level,the
R2657 T3293 T3292 arg1Of level,single-cell
R2658 T3298 T3297 arg1Of cells,Th2
R2659 T3298 T3299 arg1Of cells,lack
R2660 T3299 T3295 arg2Of lack,confirming
R2661 T3299 T3296 arg1Of lack,that
R2662 T3301 T3299 arg2Of expression,lack
R2663 T3301 T3300 arg1Of expression,FOXP3
R2664 T3301 T3302 arg1Of expression,(
R2665 T3304 T3302 arg2Of 2A,(
R2666 T3304 T3303 arg1Of 2A,Figure
R2667 T3305 T3302 arg3Of ),(
R2668 T3308 T3306 arg1Of cells,Only
R2669 T3308 T3307 arg1Of cells,iTreg
R2670 T3308 T3309 arg1Of cells,expressed
R2671 T3309 T3311 arg1Of expressed,in
R2672 T3310 T3309 arg2Of FOXP3,expressed
R2673 T3313 T3311 arg2Of conditions,in
R2674 T3313 T3312 arg1Of conditions,resting
R2675 T3316 T3317 arg1Of we,observed
R2676 T3317 T3314 arg1Of observed,Interestingly
R2677 T3317 T3315 arg1Of observed,","
R2678 T3321 T3319 arg1Of cells,resting
R2679 T3321 T3320 arg1Of cells,iTreg
R2680 T3321 T3322 arg1Of cells,express
R2681 T3321 T3325 arg1Of cells,show
R2682 T3322 T3324 arg1Of express,but
R2683 T3323 T3322 arg2Of FOXP3,express
R2684 T3324 T3317 arg2Of but,observed
R2685 T3324 T3318 arg1Of but,that
R2686 T3325 T3324 arg2Of show,but
R2687 T3329 T3325 arg2Of expression,show
R2688 T3329 T3326 arg1Of expression,low
R2689 T3329 T3327 arg1Of expression,CD25
R2690 T3329 T3328 arg1Of expression,surface
R2691 T3331 T3330 arg2Of exposure,Repeated
R2692 T3331 T3332 arg1Of exposure,to
R2693 T3331 T3334 arg1Of exposure,did
R2694 T3331 T3337 arg1Of exposure,increase
R2695 T3331 T3347 arg1Of exposure,induced
R2696 T3333 T3332 arg2Of TGF-β,to
R2697 T3337 T3334 arg2Of increase,did
R2698 T3337 T3335 arg1Of increase,not
R2699 T3337 T3336 arg1Of increase,further
R2700 T3337 T3345 arg1Of increase,but
R2701 T3340 T3337 arg2Of expression,increase
R2702 T3340 T3338 arg1Of expression,the
R2703 T3340 T3339 arg1Of expression,FOXP3
R2704 T3340 T3341 arg1Of expression,in
R2705 T3344 T3341 arg2Of lineage,in
R2706 T3344 T3342 arg1Of lineage,the
R2707 T3344 T3343 arg1Of lineage,iTreg
R2708 T3347 T3345 arg2Of induced,but
R2709 T3347 T3346 arg1Of induced,transiently
R2710 T3349 T3347 arg2Of expression,induced
R2711 T3349 T3348 arg1Of expression,FOXP3
R2712 T3349 T3350 arg1Of expression,in
R2713 T3352 T3350 arg2Of cells,in
R2714 T3352 T3351 arg1Of cells,Th1
R2715 T3354 T3353 arg1Of occurring,Naturally
R2716 T3356 T3354 arg1Of cells,occurring
R2717 T3356 T3355 arg1Of cells,Th2
R2718 T3356 T3358 arg1Of cells,as
R2719 T3356 T3372 arg1Of cells,or
R2720 T3358 T3357 arg1Of as,such
R2721 T3361 T3358 arg2Of cells,as
R2722 T3361 T3359 arg1Of cells,CRTH2+
R2723 T3361 T3360 arg1Of cells,T
R2724 T3361 T3362 arg1Of cells,","
R2725 T3364 T3362 arg2Of cells,","
R2726 T3364 T3363 arg1Of cells,T
R2727 T3364 T3365 arg2Of cells,isolated
R2728 T3365 T3366 arg1Of isolated,according
R2729 T3367 T3366 arg2Of to,according
R2730 T3370 T3367 arg2Of secretion,to
R2731 T3370 T3368 arg1Of secretion,their
R2732 T3370 T3369 arg1Of secretion,IL-4
R2733 T3372 T3371 arg1Of or,","
R2734 T3372 T3381 arg1Of or,were
R2735 T3372 T3383 arg1Of or,lacking
R2736 T3377 T3372 arg2Of clone,or
R2737 T3377 T3373 arg1Of clone,an
R2738 T3377 T3374 arg1Of clone,IL-4–producing
R2739 T3377 T3375 arg1Of clone,T
R2740 T3377 T3376 arg1Of clone,cell
R2741 T3377 T3378 arg1Of clone,(
R2742 T3379 T3378 arg2Of BR8,(
R2743 T3380 T3378 arg3Of ),(
R2744 T3383 T3381 arg2Of lacking,were
R2745 T3383 T3382 arg1Of lacking,also
R2746 T3385 T3383 arg2Of expression,lacking
R2747 T3385 T3384 arg1Of expression,FOXP3
R2748 T3385 T3386 arg1Of expression,(
R2749 T3388 T3386 arg2Of 2B,(
R2750 T3388 T3387 arg1Of 2B,Figure
R2751 T3389 T3386 arg3Of ),(
R2752 T3393 T3390 arg1Of induction,Furthermore
R2753 T3393 T3391 arg1Of induction,TGF-β–mediated
R2754 T3393 T3392 arg1Of induction,FOXP3
R2755 T3393 T3394 arg1Of induction,failed
R2756 T3394 T3395 arg1Of failed,in
R2757 T3394 T3398 arg1Of failed,in
R2758 T3397 T3395 arg2Of cells,in
R2759 T3397 T3396 arg1Of cells,these
R2760 T3398 T3400 arg1Of in,to
R2761 T3399 T3398 arg2Of contrast,in
R2762 T3404 T3398 arg3Of cells,in
R2763 T3404 T3401 arg1Of cells,the
R2764 T3404 T3402 arg1Of cells,naive
R2765 T3404 T3403 arg1Of cells,T
R2766 T3404 T3405 arg1Of cells,(
R2767 T3407 T3405 arg2Of 2B,(
R2768 T3407 T3406 arg1Of 2B,Figure
R2769 T3408 T3405 arg3Of ),(
R2770 T3410 T3411 arg1Of IL-4,is
R2771 T3411 T3409 arg2Of is,Because
R2772 T3415 T3411 arg2Of cytokine,is
R2773 T3415 T3412 arg1Of cytokine,the
R2774 T3415 T3413 arg1Of cytokine,key
R2775 T3415 T3414 arg1Of cytokine,Th2
R2776 T3418 T3417 arg1Of expression,the
R2777 T3418 T3419 arg1Of expression,of
R2778 T3418 T3423 arg1Of expression,in
R2779 T3418 T3429 arg1Of expression,was
R2780 T3418 T3430 arg2Of expression,analyzed
R2781 T3420 T3421 arg1Of IL-4,and
R2782 T3421 T3419 arg2Of and,of
R2783 T3422 T3421 arg2Of FOXP3,and
R2784 T3428 T3423 arg2Of cells,in
R2785 T3428 T3424 arg1Of cells,freshly
R2786 T3428 T3425 arg2Of cells,isolated
R2787 T3428 T3426 arg1Of cells,CD4+
R2788 T3428 T3427 arg1Of cells,T
R2789 T3430 T3409 arg1Of analyzed,Because
R2790 T3430 T3416 arg1Of analyzed,","
R2791 T3430 T3429 arg2Of analyzed,was
R2792 T3435 T3430 arg1Of sorting,analyzed
R2793 T3435 T3431 arg2Of sorting,by
R2794 T3435 T3432 arg1Of sorting,fluorescence
R2795 T3435 T3433 arg2Of sorting,activated
R2796 T3435 T3434 arg1Of sorting,cell
R2797 T3435 T3436 arg1Of sorting,(
R2798 T3437 T3436 arg2Of FACS,(
R2799 T3438 T3436 arg3Of ),(
R2800 T3440 T3439 arg1Of cells,IL-4–expressing
R2801 T3440 T3441 arg1Of cells,were
R2802 T3440 T3443 arg1Of cells,abundant
R2803 T3443 T3441 arg2Of abundant,were
R2804 T3443 T3442 arg1Of abundant,most
R2805 T3443 T3444 arg1Of abundant,among
R2806 T3446 T3444 arg2Of cells,among
R2807 T3446 T3445 arg1Of cells,CD45RO+CD25–
R2808 T3446 T3447 arg1Of cells,","
R2809 T3446 T3448 arg1Of cells,which
R2810 T3446 T3449 arg1Of cells,did
R2811 T3446 T3451 arg1Of cells,co-express
R2812 T3451 T3449 arg2Of co-express,did
R2813 T3451 T3450 arg1Of co-express,not
R2814 T3452 T3451 arg2Of FOXP3,co-express
R2815 T3452 T3453 arg1Of FOXP3,(
R2816 T3455 T3453 arg2Of 3A,(
R2817 T3455 T3454 arg1Of 3A,Figure
R2818 T3455 T3456 arg1Of 3A,","
R2819 T3458 T3456 arg2Of panel,","
R2820 T3458 T3457 arg1Of panel,left
R2821 T3459 T3453 arg3Of ),(
R2822 T3461 T3460 arg2Of contrast,In
R2823 T3464 T3463 arg1Of cells,CD45RO+CD25+
R2824 T3464 T3466 arg1Of cells,expressed
R2825 T3464 T3470 arg1Of cells,lacking
R2826 T3466 T3460 arg1Of expressed,In
R2827 T3466 T3462 arg1Of expressed,","
R2828 T3466 T3465 arg1Of expressed,abundantly
R2829 T3466 T3468 arg1Of expressed,","
R2830 T3466 T3469 arg1Of expressed,while
R2831 T3467 T3466 arg2Of FOXP3,expressed
R2832 T3470 T3469 arg2Of lacking,while
R2833 T3471 T3470 arg2Of IL-4,lacking
R2834 T3471 T3472 arg1Of IL-4,(
R2835 T3474 T3472 arg2Of 3A,(
R2836 T3474 T3473 arg1Of 3A,Figure
R2837 T3474 T3475 arg1Of 3A,","
R2838 T3477 T3475 arg2Of panel,","
R2839 T3477 T3476 arg1Of panel,right
R2840 T3478 T3472 arg3Of ),(
R2841 T3480 T3479 arg2Of shown,As
R2842 T3480 T3481 arg1Of shown,in
R2843 T3483 T3481 arg2Of 3B,in
R2844 T3483 T3482 arg1Of 3B,Figure
R2845 T3486 T3485 arg1Of frequency,the
R2846 T3486 T3487 arg1Of frequency,of
R2847 T3486 T3491 arg1Of frequency,was
R2848 T3486 T3493 arg1Of frequency,below
R2849 T3490 T3487 arg2Of cells,of
R2850 T3490 T3488 arg1Of cells,the
R2851 T3490 T3489 arg1Of cells,IL-4+
R2852 T3491 T3479 arg1Of was,As
R2853 T3491 T3484 arg1Of was,","
R2854 T3491 T3492 arg1Of was,always
R2855 T3491 T3496 arg1Of was,in
R2856 T3493 T3491 arg2Of below,was
R2857 T3495 T3493 arg2Of %,below
R2858 T3495 T3494 arg1Of %,1
R2859 T3499 T3496 arg2Of cells,in
R2860 T3499 T3497 arg1Of cells,the
R2861 T3499 T3498 arg1Of cells,FOXP3+
R2862 T3499 T3500 arg1Of cells,close
R2863 T3500 T3501 arg1Of close,to
R2864 T3503 T3501 arg2Of background,to
R2865 T3503 T3502 arg1Of background,the
R2866 T3506 T3504 arg1Of cells,The
R2867 T3506 T3505 arg1Of cells,IL-4+
R2868 T3506 T3507 arg1Of cells,were
R2869 T3506 T3508 arg2Of cells,confined
R2870 T3508 T3507 arg2Of confined,were
R2871 T3508 T3509 arg1Of confined,to
R2872 T3508 T3513 arg1Of confined,","
R2873 T3508 T3514 arg1Of confined,as
R2874 T3512 T3509 arg2Of cells,to
R2875 T3512 T3510 arg1Of cells,the
R2876 T3512 T3511 arg1Of cells,FOXP3–
R2877 T3515 T3514 arg2Of shown,as
R2878 T3515 T3516 arg1Of shown,for
R2879 T3518 T3519 arg1Of CD45RO+CD25–,","
R2880 T3520 T3522 arg1Of CD45RO+CD25+,and
R2881 T3522 T3519 arg2Of and,","
R2882 T3522 T3521 arg1Of and,","
R2883 T3523 T3522 arg2Of CD45RO+CD25+high,and
R2884 T3524 T3516 arg2Of cells,for
R2885 T3524 T3517 arg1Of cells,the
R2886 T3524 T3518 arg1Of cells,CD45RO+CD25–
R2887 T3524 T3520 arg1Of cells,CD45RO+CD25+
R2888 T3524 T3523 arg1Of cells,CD45RO+CD25+high
R2889 T3524 T3525 arg1Of cells,(
R2890 T3527 T3525 arg2Of 3B,(
R2891 T3527 T3526 arg1Of 3B,Figure
R2892 T3528 T3525 arg3Of ),(
R2893 T3530 T3529 arg2Of addition,In
R2894 T3535 T3534 arg1Of clone,Th2
R2895 T3535 T3536 arg1Of clone,(
R2896 T3535 T3539 arg1Of clone,nor
R2897 T3537 T3536 arg2Of BR8,(
R2898 T3538 T3536 arg3Of ),(
R2899 T3539 T3532 arg1Of nor,neither
R2900 T3539 T3533 arg1Of nor,the
R2901 T3539 T3543 arg1Of nor,expressed
R2902 T3541 T3539 arg2Of cells,nor
R2903 T3541 T3540 arg1Of cells,CRTH2
R2904 T3543 T3529 arg1Of expressed,In
R2905 T3543 T3531 arg1Of expressed,","
R2906 T3543 T3542 arg1Of expressed,significantly
R2907 T3544 T3543 arg2Of FOXP3,expressed
R2908 T3544 T3545 arg1Of FOXP3,(
R2909 T3547 T3545 arg2Of 3C,(
R2910 T3547 T3546 arg1Of 3C,Figure
R2911 T3548 T3545 arg3Of ),(
R2912 T3549 T3550 arg2Of Cells,enriched
R2913 T3549 T3561 arg1Of Cells,contained
R2914 T3550 T3551 arg1Of enriched,for
R2915 T3554 T3551 arg2Of secretion,for
R2916 T3554 T3552 arg1Of secretion,their
R2917 T3554 T3553 arg1Of secretion,IL-4
R2918 T3554 T3555 arg1Of secretion,using
R2919 T3560 T3555 arg2Of technology,using
R2920 T3560 T3556 arg1Of technology,the
R2921 T3560 T3557 arg1Of technology,magnetic
R2922 T3560 T3558 arg1Of technology,cell
R2923 T3560 T3559 arg1Of technology,isolation
R2924 T3561 T3566 arg1Of contained,but
R2925 T3564 T3561 arg2Of cells,contained
R2926 T3564 T3562 arg1Of cells,some
R2927 T3564 T3563 arg1Of cells,FOXP3-expressing
R2928 T3566 T3565 arg1Of but,","
R2929 T3570 T3569 arg1Of expression,the
R2930 T3570 T3571 arg1Of expression,did
R2931 T3570 T3573 arg1Of expression,overlap
R2932 T3573 T3566 arg2Of overlap,but
R2933 T3573 T3567 arg1Of overlap,importantly
R2934 T3573 T3568 arg1Of overlap,","
R2935 T3573 T3571 arg2Of overlap,did
R2936 T3573 T3572 arg1Of overlap,not
R2937 T3574 T3575 arg1Of Taken,together
R2938 T3578 T3577 arg1Of data,these
R2939 T3578 T3579 arg1Of data,indicate
R2940 T3579 T3574 modOf indicate,Taken
R2941 T3579 T3576 arg1Of indicate,","
R2942 T3581 T3582 arg1Of FOXP3,was
R2943 T3581 T3584 arg2Of FOXP3,expressed
R2944 T3581 T3589 arg1Of FOXP3,was
R2945 T3581 T3591 arg1Of FOXP3,inducible
R2946 T3584 T3582 arg2Of expressed,was
R2947 T3584 T3583 arg1Of expressed,not
R2948 T3584 T3588 arg1Of expressed,and
R2949 T3587 T3584 arg1Of cells,expressed
R2950 T3587 T3585 arg2Of cells,by
R2951 T3587 T3586 arg1Of cells,Th2
R2952 T3588 T3579 arg2Of and,indicate
R2953 T3588 T3580 arg1Of and,that
R2954 T3589 T3588 arg2Of was,and
R2955 T3589 T3590 arg1Of was,not
R2956 T3589 T3592 arg1Of was,in
R2957 T3591 T3589 arg2Of inducible,was
R2958 T3594 T3592 arg2Of cells,in
R2959 T3594 T3593 arg1Of cells,those
R3995 T5221 T5222 arg1Of FOXP3,and
R3996 T5222 T5225 arg1Of and,in
R3997 T5224 T5222 arg2Of Kinetics,and
R3998 T5224 T5223 arg1Of Kinetics,GATA3
R3999 T5227 T5225 arg2Of Cells,in
R4000 T5227 T5226 arg1Of Cells,Differentiating
R4001 T5230 T5228 arg1Of capacity,The
R4002 T5230 T5229 arg2Of capacity,limited
R4003 T5230 T5231 arg1Of capacity,of
R4004 T5230 T5235 modOf capacity,to
R4005 T5230 T5239 arg1Of capacity,suggests
R4006 T5234 T5231 arg2Of cells,of
R4007 T5234 T5232 arg2Of cells,differentiated
R4008 T5234 T5233 arg1Of cells,effector
R4009 T5236 T5235 arg1Of induce,to
R4010 T5238 T5236 arg2Of expression,induce
R4011 T5238 T5237 arg1Of expression,FOXP3
R4012 T5242 T5241 arg1Of induction,iTreg
R4013 T5242 T5243 arg1Of induction,has
R4014 T5242 T5245 arg1Of induction,occur
R4015 T5243 T5239 arg2Of has,suggests
R4016 T5243 T5240 arg1Of has,that
R4017 T5245 T5243 arg2Of occur,has
R4018 T5245 T5244 arg1Of occur,to
R4019 T5245 T5246 arg1Of occur,before
R4020 T5250 T5247 arg1Of differentiation,effector
R4021 T5250 T5248 arg1Of differentiation,T
R4022 T5250 T5249 arg1Of differentiation,cell
R4023 T5250 T5251 arg1Of differentiation,occurs
R4024 T5251 T5246 arg2Of occurs,before
R4025 T5254 T5255 arg1Of we,analyzed
R4026 T5255 T5252 arg1Of analyzed,Therefore
R4027 T5255 T5253 arg1Of analyzed,","
R4028 T5255 T5262 arg1Of analyzed,during
R4029 T5257 T5255 arg2Of expression,analyzed
R4030 T5257 T5256 arg1Of expression,the
R4031 T5257 T5258 arg1Of expression,of
R4032 T5259 T5260 arg1Of FOXP3,and
R4033 T5260 T5258 arg2Of and,of
R4034 T5261 T5260 arg2Of GATA3,and
R4035 T5264 T5262 arg2Of differentiation,during
R4036 T5264 T5263 arg1Of differentiation,the
R4037 T5264 T5265 arg1Of differentiation,of
R4038 T5264 T5270 arg1Of differentiation,into
R4039 T5269 T5265 arg2Of cells,of
R4040 T5269 T5266 arg1Of cells,naive
R4041 T5269 T5267 arg1Of cells,CD4
R4042 T5269 T5268 arg1Of cells,T
R4043 T5271 T5272 arg1Of Th0,(
R4044 T5271 T5283 arg1Of Th0,","
R4045 T5273 T5274 arg1Of neutral,","
R4046 T5274 T5276 arg1Of ",",","
R4047 T5275 T5274 arg2Of anti-IL-4,","
R4048 T5276 T5279 arg1Of ",",and
R4049 T5277 T5276 arg2Of anti-IFN-γ,","
R4050 T5279 T5278 arg1Of and,","
R4051 T5280 T5279 arg2Of anti,and
R4052 T5281 T5272 arg2Of IL-12,(
R4053 T5281 T5273 arg1Of IL-12,neutral
R4054 T5281 T5275 arg1Of IL-12,anti-IL-4
R4055 T5281 T5277 arg1Of IL-12,anti-IFN-γ
R4056 T5281 T5280 arg1Of IL-12,anti
R4057 T5282 T5272 arg3Of ),(
R4058 T5283 T5286 arg1Of ",",and
R4059 T5284 T5283 arg2Of Th2,","
R4060 T5286 T5285 arg1Of and,","
R4061 T5287 T5286 arg2Of iTreg,and
R4062 T5288 T5270 arg2Of phenotypes,into
R4063 T5288 T5271 arg1Of phenotypes,Th0
R4064 T5288 T5284 arg1Of phenotypes,Th2
R4065 T5288 T5287 arg1Of phenotypes,iTreg
R4066 T5290 T5289 arg2Of initiation,After
R4067 T5290 T5291 arg1Of initiation,of
R4068 T5294 T5291 arg2Of process,of
R4069 T5294 T5292 arg1Of process,the
R4070 T5294 T5293 arg1Of process,differentiation
R4071 T5296 T5297 arg1Of FOXP3,and
R4072 T5297 T5299 arg1Of and,showed
R4073 T5298 T5297 arg2Of GATA3,and
R4074 T5299 T5289 arg1Of showed,After
R4075 T5299 T5295 arg1Of showed,","
R4076 T5302 T5299 arg2Of expression,showed
R4077 T5302 T5300 arg1Of expression,a
R4078 T5302 T5301 arg1Of expression,similar
R4079 T5302 T5303 arg1Of expression,kinetic
R4080 T5303 T5304 arg1Of kinetic,within
R4081 T5308 T5304 arg2Of d,within
R4082 T5308 T5305 arg1Of d,the
R4083 T5308 T5306 arg1Of d,first
R4084 T5308 T5307 arg1Of d,3
R4085 T5308 T5309 arg1Of d,","
R4086 T5308 T5310 arg1Of d,which
R4087 T5308 T5311 arg1Of d,are
R4088 T5308 T5312 arg2Of d,considered
R4089 T5308 T5314 arg1Of d,be
R4090 T5308 T5315 arg1Of d,critical
R4091 T5312 T5311 arg2Of considered,are
R4092 T5314 T5312 arg3Of be,considered
R4093 T5314 T5313 arg1Of be,to
R4094 T5315 T5314 arg2Of critical,be
R4095 T5315 T5316 arg1Of critical,in
R4096 T5319 T5316 arg2Of commitment,in
R4097 T5319 T5317 arg1Of commitment,T
R4098 T5319 T5318 arg1Of commitment,cell
R4099 T5319 T5320 arg1Of commitment,[
R4100 T5321 T5320 arg2Of 18,[
R4101 T5322 T5320 arg3Of ],[
R4102 T5326 T5323 arg2Of conditions,Under
R4103 T5326 T5324 arg1Of conditions,Th2
R4104 T5326 T5325 arg1Of conditions,differentiation
R4105 T5330 T5328 arg1Of expression,FOXP3
R4106 T5330 T5329 arg1Of expression,mRNA
R4107 T5330 T5331 arg1Of expression,increased
R4108 T5331 T5323 arg1Of increased,Under
R4109 T5331 T5327 arg1Of increased,","
R4110 T5331 T5333 arg1Of increased,marginally
R4111 T5333 T5332 arg1Of marginally,only
R4112 T5333 T5334 arg1Of marginally,(
R4113 T5336 T5334 arg2Of 4A,(
R4114 T5336 T5335 arg1Of 4A,Figure
R4115 T5336 T5337 arg1Of 4A,","
R4116 T5339 T5337 arg2Of panel,","
R4117 T5339 T5338 arg1Of panel,left
R4118 T5340 T5334 arg3Of ),(
R4119 T5344 T5345 arg1Of GATA3,and
R4120 T5345 T5347 arg1Of and,showed
R4121 T5346 T5345 arg2Of FOXP3,and
R4122 T5347 T5343 arg2Of showed,although
R4123 T5349 T5347 arg2Of kinetics,showed
R4124 T5349 T5348 arg1Of kinetics,similar
R4125 T5352 T5351 arg1Of expression,their
R4126 T5352 T5353 arg1Of expression,polarizes
R4127 T5353 T5341 arg1Of polarizes,Thus
R4128 T5353 T5342 arg1Of polarizes,","
R4129 T5353 T5343 arg1Of polarizes,although
R4130 T5353 T5350 arg1Of polarizes,","
R4131 T5353 T5354 arg1Of polarizes,at
R4132 T5353 T5361 arg1Of polarizes,when
R4133 T5356 T5354 arg2Of end,at
R4134 T5356 T5355 arg1Of end,the
R4135 T5356 T5357 arg1Of end,of
R4136 T5360 T5357 arg2Of process,of
R4137 T5360 T5358 arg1Of process,the
R4138 T5360 T5359 arg1Of process,differentiation
R4139 T5362 T5363 arg1Of cells,were
R4140 T5362 T5364 arg2Of cells,cultured
R4141 T5364 T5361 arg2Of cultured,when
R4142 T5364 T5363 arg2Of cultured,were
R4143 T5364 T5365 arg1Of cultured,towards
R4144 T5366 T5367 arg1Of Th2,or
R4145 T5368 T5367 arg2Of iTreg,or
R4146 T5369 T5365 arg2Of cells,towards
R4147 T5369 T5366 arg1Of cells,Th2
R4148 T5369 T5368 arg1Of cells,iTreg
R4149 T5369 T5370 arg1Of cells,","
R4150 T5369 T5371 arg1Of cells,respectively
R4151 T5369 T5372 arg1Of cells,(
R4152 T5374 T5373 arg1Of 4A,Figure
R4153 T5374 T5375 arg1Of 4A,and
R4154 T5375 T5372 arg2Of and,(
R4155 T5376 T5375 arg2Of 4B,and
R4156 T5377 T5372 arg3Of ),(
R4157 T5382 T5380 arg1Of cells,the
R4158 T5382 T5381 arg1Of cells,Th0
R4159 T5382 T5383 arg1Of cells,were
R4160 T5382 T5384 arg1Of cells,expressing
R4161 T5382 T5393 arg1Of cells,expressed
R4162 T5384 T5383 arg2Of expressing,were
R4163 T5384 T5392 arg1Of expressing,but
R4164 T5386 T5384 arg2Of FOXP3,expressing
R4165 T5386 T5385 arg1Of FOXP3,more
R4166 T5386 T5387 arg1Of FOXP3,than
R4167 T5390 T5387 arg2Of cells,than
R4168 T5390 T5388 arg1Of cells,the
R4169 T5390 T5389 arg1Of cells,Th2
R4170 T5392 T5378 arg1Of but,Interestingly
R4171 T5392 T5379 arg1Of but,","
R4172 T5392 T5391 arg1Of but,","
R4173 T5393 T5392 arg2Of expressed,but
R4174 T5393 T5398 arg1Of expressed,;
R4175 T5395 T5393 arg2Of levels,expressed
R4176 T5395 T5394 arg1Of levels,low
R4177 T5395 T5396 arg1Of levels,of
R4178 T5397 T5396 arg2Of GATA3,of
R4179 T5403 T5401 arg1Of expression,the
R4180 T5403 T5402 arg1Of expression,protein
R4181 T5403 T5405 arg1Of expression,differed
R4182 T5405 T5398 arg2Of differed,;
R4183 T5405 T5399 arg1Of differed,however
R4184 T5405 T5400 arg1Of differed,","
R4185 T5405 T5404 arg1Of differed,slightly
R4186 T5405 T5406 arg1Of differed,from
R4187 T5405 T5409 arg1Of differed,","
R4188 T5408 T5406 arg2Of expression,from
R4189 T5408 T5407 arg1Of expression,mRNA
R4190 T5410 T5405 arg2Of suggesting,differed
R4191 T5410 T5416 arg1Of suggesting,as
R4192 T5412 T5411 arg1Of posttranslational,also
R4193 T5413 T5414 arg1Of regulation,and
R4194 T5414 T5410 arg2Of and,suggesting
R4195 T5414 T5412 arg1Of and,posttranslational
R4196 T5415 T5414 arg2Of degradation,and
R4197 T5419 T5416 arg2Of mechanisms,as
R4198 T5419 T5417 arg1Of mechanisms,potential
R4199 T5419 T5418 arg1Of mechanisms,additional
R4200 T5419 T5420 arg1Of mechanisms,in
R4201 T5423 T5420 arg2Of process,in
R4202 T5423 T5421 arg1Of process,the
R4203 T5423 T5422 arg1Of process,differentiation
R4204 T5425 T5424 arg1Of phenotype,The
R4205 T5425 T5426 arg1Of phenotype,of
R4206 T5425 T5429 arg1Of phenotype,included
R4207 T5428 T5426 arg2Of cells,of
R4208 T5428 T5427 arg1Of cells,iTreg
R4209 T5432 T5429 arg2Of phenotype,included
R4210 T5432 T5430 arg1Of phenotype,an
R4211 T5432 T5431 arg1Of phenotype,anergic
R4212 T5432 T5433 arg1Of phenotype,upon
R4213 T5435 T5434 arg1Of re-stimulation,anti-CD3
R4214 T5435 T5436 arg1Of re-stimulation,(
R4215 T5435 T5440 arg1Of re-stimulation,","
R4216 T5438 T5436 arg2Of S1A,(
R4217 T5438 T5437 arg1Of S1A,Figure
R4218 T5439 T5436 arg3Of ),(
R4219 T5440 T5433 arg2Of ",",upon
R4220 T5441 T5442 arg1Of CD103,","
R4221 T5442 T5444 arg1Of ",",","
R4222 T5443 T5442 arg2Of CTLA-4,","
R4223 T5444 T5447 arg1Of ",",and
R4224 T5445 T5444 arg2Of GITR,","
R4225 T5447 T5440 arg2Of and,","
R4226 T5447 T5446 arg1Of and,","
R4227 T5450 T5447 arg2Of expression,and
R4228 T5450 T5448 arg1Of expression,PD-1
R4229 T5450 T5449 arg1Of expression,surface
R4230 T5450 T5451 arg1Of expression,(
R4231 T5453 T5451 arg2Of S1B,(
R4232 T5453 T5452 arg1Of S1B,Figure
R4233 T5454 T5451 arg3Of ),(
R4234 T5458 T5455 arg2Of level,On
R4235 T5458 T5456 arg1Of level,the
R4236 T5458 T5457 arg1Of level,single-cell
R4237 T5460 T5461 arg1Of it,can
R4238 T5460 T5462 arg1Of it,be
R4239 T5460 T5463 arg2Of it,seen
R4240 T5463 T5461 arg2Of seen,can
R4241 T5463 T5462 arg2Of seen,be
R4242 T5465 T5464 arg1Of cells,that
R4243 T5465 T5466 arg1Of cells,progress
R4244 T5466 T5455 arg1Of progress,On
R4245 T5466 T5459 arg1Of progress,","
R4246 T5466 T5461 modOf progress,can
R4247 T5466 T5467 arg1Of progress,through
R4248 T5466 T5481 arg1Of progress,in
R4249 T5470 T5467 arg2Of phase,through
R4250 T5470 T5468 arg1Of phase,a
R4251 T5470 T5469 arg1Of phase,transition
R4252 T5470 T5471 arg1Of phase,","
R4253 T5470 T5472 arg1Of phase,where
R4254 T5473 T5474 arg1Of GATA3,and
R4255 T5475 T5474 arg2Of FOXP3,and
R4256 T5476 T5473 arg1Of expression,GATA3
R4257 T5476 T5475 arg1Of expression,FOXP3
R4258 T5476 T5477 arg1Of expression,coexist
R4259 T5477 T5472 arg2Of coexist,where
R4260 T5477 T5478 arg1Of coexist,to
R4261 T5480 T5478 arg2Of degree,to
R4262 T5480 T5479 arg1Of degree,some
R4263 T5484 T5481 arg2Of cells,in
R4264 T5484 T5482 arg1Of cells,the
R4265 T5484 T5483 arg1Of cells,same
R4266 T5484 T5485 arg1Of cells,","
R4267 T5484 T5486 arg1Of cells,which
R4268 T5484 T5487 arg1Of cells,is
R4269 T5484 T5488 arg2Of cells,resolved
R4270 T5488 T5487 arg2Of resolved,is
R4271 T5488 T5489 arg1Of resolved,in
R4272 T5488 T5492 arg1Of resolved,after
R4273 T5491 T5489 arg2Of cells,in
R4274 T5491 T5490 arg1Of cells,iTreg
R4275 T5494 T5492 arg2Of d,after
R4276 T5494 T5493 arg1Of d,7
R4277 T5494 T5495 arg1Of d,(
R4278 T5497 T5495 arg2Of 4B,(
R4279 T5497 T5496 arg1Of 4B,Figure
R4280 T5498 T5495 arg3Of ),(
R4281 T5499 T5500 arg1Of Taken,together
R4282 T5503 T5502 arg1Of data,these
R4283 T5503 T5504 arg1Of data,demonstrated
R4284 T5504 T5499 modOf demonstrated,Taken
R4285 T5504 T5501 arg1Of demonstrated,","
R4286 T5507 T5506 arg1Of cells,Th2
R4287 T5507 T5508 arg1Of cells,have
R4288 T5507 T5509 arg1Of cells,lost
R4289 T5507 T5516 arg1Of cells,showed
R4290 T5509 T5508 arg2Of lost,have
R4291 T5509 T5515 arg1Of lost,and
R4292 T5511 T5509 arg2Of capacity,lost
R4293 T5511 T5510 arg1Of capacity,their
R4294 T5511 T5512 modOf capacity,to
R4295 T5513 T5512 arg1Of express,to
R4296 T5514 T5513 arg2Of FOXP3,express
R4297 T5515 T5504 arg2Of and,demonstrated
R4298 T5515 T5505 arg1Of and,that
R4299 T5516 T5515 arg2Of showed,and
R4300 T5518 T5519 arg1Of Th2,and
R4301 T5520 T5519 arg2Of iTreg,and
R4302 T5521 T5518 arg1Of cells,Th2
R4303 T5521 T5520 arg1Of cells,iTreg
R4304 T5521 T5522 arg1Of cells,arise
R4305 T5522 T5516 arg2Of arise,showed
R4306 T5522 T5517 arg1Of arise,that
R4307 T5522 T5523 arg1Of arise,from
R4308 T5527 T5523 arg2Of pathways,from
R4309 T5527 T5524 arg1Of pathways,two
R4310 T5527 T5525 arg1Of pathways,different
R4311 T5527 T5526 arg1Of pathways,differentiation
R4884 T6489 T6490 arg1Of IL-4,Inhibits
R4885 T6493 T6490 arg2Of Commitment,Inhibits
R4886 T6493 T6491 arg1Of Commitment,TGF-β-Mediated
R4887 T6493 T6492 arg1Of Commitment,iTreg
R4888 T6494 T6495 arg1Of IL-4,induces
R4889 T6495 T6501 arg1Of induces,","
R4890 T6495 T6502 arg1Of induces,upon
R4891 T6495 T6505 arg1Of induces,","
R4892 T6495 T6506 arg1Of induces,into
R4893 T6496 T6495 arg2Of differentiation,induces
R4895 T6496 T6497 arg1Of differentiation,of
R4896 T6500 T6497 arg2Of cells,of
R4900 T6500 T6498 arg1Of cells,naive
R4902 T6500 T6499 arg1Of cells,T
R4903 T6504 T6502 arg2Of encounter,upon
R4907 T6504 T6503 arg1Of encounter,antigen
R4909 T6510 T6506 arg2Of lineage,into
R4919 T6510 T6507 arg1Of lineage,the
R4921 T6510 T6508 arg1Of lineage,Th2
R4926 T6510 T6509 arg1Of lineage,cell
R4927 T6511 T6513 arg1Of We,asked
R4929 T6513 T6512 arg1Of asked,therefore
R4933 T6515 T6516 arg1Of IL-4,is
R4935 T6515 T6517 arg1Of IL-4,able
R4937 T6515 T6519 arg1Of IL-4,inhibit
R4938 T6516 T6513 arg2Of is,asked
R4939 T6516 T6514 arg1Of is,whether
R4940 T6517 T6516 arg2Of able,is
R4942 T6519 T6517 arg2Of inhibit,able
R4943 T6519 T6518 arg1Of inhibit,to
R4944 T6519 T6524 arg1Of inhibit,during
R4949 T6521 T6519 arg2Of induction,inhibit
R4953 T6521 T6520 arg1Of induction,TGF-β
R4954 T6521 T6522 arg1Of induction,of
R4955 T6523 T6522 arg2Of FOXP3,of
R4956 T6526 T6524 arg2Of priming,during
R4957 T6526 T6525 arg1Of priming,the
R4958 T6526 T6527 arg1Of priming,of
R4959 T6530 T6527 arg2Of cells,of
R4960 T6547 T6535 arg2Of and,were
R4961 T6530 T6528 arg1Of cells,naive
R4962 T6548 T6547 arg2Of harvested,and
R4963 T6548 T6549 arg1Of harvested,after
R4964 T6530 T6529 arg1Of cells,T
R4965 T6534 T6531 arg1Of cells,Human
R4966 T6551 T6549 arg2Of d,after
R4967 T6551 T6550 arg1Of d,5
R4968 T6552 T6554 arg1Of IL-4,repressed
R4969 T6534 T6532 arg1Of cells,CD4+CD45RA+
R4970 T6554 T6553 arg1Of repressed,efficiently
R4971 T6554 T6565 arg1Of repressed,in
R4972 T6534 T6533 arg1Of cells,T
R4973 T6534 T6535 arg1Of cells,were
R4974 T6534 T6536 arg2Of cells,activated
R4975 T6557 T6554 arg2Of induction,repressed
R4976 T6557 T6555 arg1Of induction,the
R4977 T6557 T6556 arg1Of induction,TGF-β–mediated
R4978 T6557 T6558 arg1Of induction,of
R4979 T6534 T6548 arg2Of cells,harvested
R4980 T6536 T6537 arg1Of activated,with
R4981 T6560 T6558 arg2Of expression,of
R4982 T6560 T6559 arg1Of expression,FOXP3
R4983 T6560 T6561 arg1Of expression,(
R4984 T6563 T6561 arg2Of 5A,(
R4985 T6563 T6562 arg1Of 5A,Figure
R4986 T6536 T6540 arg1Of activated,in
R4987 T6536 T6547 arg1Of activated,and
R4988 T6539 T6537 arg2Of anti-CD3/CD28,with
R4989 T6564 T6561 arg3Of ),(
R4990 T6568 T6565 arg2Of manner,in
R4991 T6568 T6566 arg1Of manner,a
R4992 T6568 T6567 arg1Of manner,concentration-dependent
R4993 T6568 T6569 arg1Of manner,(
R4994 T6571 T6569 arg2Of 5B,(
R4995 T6571 T6570 arg1Of 5B,Figure
R4996 T6539 T6538 arg1Of anti-CD3/CD28,plate-bound
R4997 T6572 T6569 arg3Of ),(
R4998 T6542 T6540 arg2Of presence,in
R4999 T6574 T6573 arg1Of levels,Low
R5000 T6574 T6575 arg1Of levels,of
R5001 T6574 T6577 arg1Of levels,were
R5002 T6574 T6578 arg2Of levels,induced
R5003 T6576 T6575 arg2Of GATA3,of
R5004 T6542 T6541 arg1Of presence,the
R5005 T6578 T6577 arg2Of induced,were
R5006 T6578 T6579 arg1Of induced,also
R5007 T6578 T6580 arg1Of induced,in
R5008 T6578 T6585 arg1Of induced,","
R5009 T6578 T6586 arg1Of induced,as
R5010 T6582 T6580 arg2Of absence,in
R5011 T6582 T6581 arg1Of absence,the
R5012 T6582 T6583 arg1Of absence,of
R5013 T6584 T6583 arg2Of IL-4,of
R5014 T6542 T6543 arg1Of presence,of
R5015 T6544 T6545 arg1Of TGF-β,and/or
R5016 T6587 T6588 arg1Of it,was
R5017 T6587 T6590 arg2Of it,observed
R5018 T6545 T6543 arg2Of and/or,of
R5019 T6590 T6586 arg2Of observed,as
R5020 T6590 T6588 arg2Of observed,was
R5021 T6590 T6589 arg1Of observed,previously
R5022 T6590 T6591 arg1Of observed,[
R5023 T6592 T6591 arg2Of 3,[
R5024 T6593 T6591 arg3Of ],[
R5025 T6546 T6545 arg2Of IL-4,and/or
R5026 T6644 T6641 arg2Of signaling,of
R5027 T6598 T6596 arg2Of concentration,at
R5028 T6598 T6597 arg1Of concentration,low
R5029 T6600 T6601 arg1Of IL-4,was
R5030 T6600 T6602 arg1Of IL-4,able
R5031 T6600 T6605 arg1Of IL-4,induce
R5032 T6601 T6594 arg1Of was,However
R5033 T6601 T6595 arg1Of was,","
R5034 T6601 T6596 arg1Of was,at
R5035 T6601 T6599 arg1Of was,","
R5036 T6602 T6601 arg2Of able,was
R5037 T6644 T6642 arg1Of signaling,the
R5038 T6644 T6643 arg1Of signaling,receptor
R5039 T6605 T6602 arg2Of induce,able
R5040 T6605 T6603 arg1Of induce,to
R5041 T6605 T6604 arg1Of induce,marginally
R5042 T6607 T6605 arg2Of expression,induce
R5043 T6607 T6606 arg1Of expression,FOXP3
R5044 T6609 T6608 arg2Of note,Of
R5045 T6648 T6647 arg1Of phosphorylation,the
R5046 T6611 T6612 arg1Of GATA3,was
R5047 T6611 T6614 arg2Of GATA3,induced
R5048 T6614 T6608 arg1Of induced,Of
R5049 T6614 T6610 arg1Of induced,","
R5050 T6614 T6612 arg2Of induced,was
R5051 T6614 T6613 arg1Of induced,also
R5052 T6614 T6615 arg1Of induced,in
R5053 T6617 T6615 arg2Of presence,in
R5054 T6617 T6616 arg1Of presence,the
R5055 T6617 T6618 arg1Of presence,of
R5056 T6617 T6620 arg1Of presence,at
R5057 T6619 T6618 arg2Of TGF-β,of
R5058 T6648 T6649 arg1Of phosphorylation,of
R5059 T6648 T6653 arg1Of phosphorylation,was
R5060 T6648 T6655 arg2Of phosphorylation,affected
R5061 T6623 T6620 arg2Of concentration,at
R5062 T6623 T6621 arg1Of concentration,high
R5063 T6623 T6622 arg1Of concentration,IL-4
R5064 T6650 T6651 arg1Of SMAD2,or
R5065 T6623 T6624 arg1Of concentration,(
R5066 T6651 T6649 arg2Of or,of
R5067 T6626 T6625 arg1Of 5A,Figure
R5068 T6626 T6627 arg1Of 5A,and
R5069 T6627 T6624 arg2Of and,(
R5070 T6652 T6651 arg2Of STAT6,or
R5071 T6628 T6627 arg2Of 5B,and
R5072 T6629 T6624 arg3Of ),(
R5073 T6655 T6646 arg2Of affected,because
R5074 T6632 T6630 arg1Of prevention,The
R5075 T6632 T6631 arg1Of prevention,IL-4–mediated
R5076 T6632 T6633 arg1Of prevention,of
R5077 T6632 T6636 arg1Of prevention,was
R5078 T6632 T6638 arg2Of prevention,caused
R5079 T6635 T6633 arg2Of expression,of
R5080 T6635 T6634 arg1Of expression,FOXP3
R5081 T6655 T6653 arg2Of affected,was
R5082 T6655 T6654 arg1Of affected,not
R5083 T6638 T6636 arg2Of caused,was
R5084 T6638 T6637 arg1Of caused,not
R5085 T6638 T6645 arg1Of caused,","
R5086 T6638 T6646 arg1Of caused,because
R5087 T6658 T6655 arg1Of addition,affected
R5088 T6640 T6638 arg1Of interferences,caused
R5089 T6640 T6639 arg2Of interferences,by
R5090 T6640 T6641 arg1Of interferences,of
R5091 T6658 T6656 arg2Of addition,by
R5092 T6658 T6657 arg1Of addition,the
R5093 T6658 T6659 arg1Of addition,of
R5094 T6658 T6663 arg1Of addition,","
R5095 T6658 T6664 arg1Of addition,which
R5096 T6658 T6665 arg1Of addition,demonstrates
R5097 T6660 T6661 arg1Of IL-4,and/or
R5098 T6661 T6659 arg2Of and/or,of
R5099 T6741 T6739 arg2Of CD25,express
R5100 T6741 T6740 arg1Of CD25,surface
R5101 T6742 T6743 arg1Of We,used
R5102 T6662 T6661 arg2Of TGF-β,and/or
R5103 T6744 T6745 arg1Of complexes,of
R5104 T6744 T6752 arg1Of complexes,plus
R5105 T6667 T6670 arg1Of IL-4,as
R5106 T6748 T6745 arg2Of IL-4,of
R5107 T6748 T6746 arg1Of IL-4,recombinant
R5108 T6748 T6747 arg1Of IL-4,mouse
R5109 T6748 T6749 arg1Of IL-4,(
R5110 T6750 T6749 arg2Of rmIL-4,(
R5111 T6751 T6749 arg3Of ),(
R5112 T6752 T6743 arg2Of plus,used
R5113 T6670 T6668 arg1Of as,as
R5114 T6755 T6752 arg2Of antibodies,plus
R5115 T6755 T6753 arg1Of antibodies,anti-IL-4
R5116 T6755 T6754 arg1Of antibodies,monoclonal
R5117 T6755 T6756 arg1Of antibodies,(
R5118 T6755 T6759 arg1Of antibodies,","
R5119 T6755 T6760 arg1Of antibodies,which
R5120 T6755 T6761 arg1Of antibodies,have
R5121 T6755 T6762 arg1Of antibodies,been
R5122 T6755 T6763 arg2Of antibodies,shown
R5123 T6755 T6766 arg1Of antibodies,increase
R5124 T6757 T6756 arg2Of mAbs,(
R5125 T6758 T6756 arg3Of ),(
R5126 T6670 T6669 arg1Of as,well
R5127 T6670 T6673 arg1Of as,were
R5128 T6763 T6761 arg2Of shown,have
R5129 T6763 T6762 arg2Of shown,been
R5130 T6670 T6674 arg1Of as,functional
R5131 T6672 T6670 arg2Of signaling,as
R5132 T6763 T6774 arg1Of shown,[
R5133 T6672 T6671 arg1Of signaling,TGF-β
R5134 T6766 T6763 arg3Of increase,shown
R5135 T6766 T6764 arg1Of increase,to
R5136 T6766 T6765 arg1Of increase,dramatically
R5137 T6673 T6665 arg2Of were,demonstrates
R5138 T6768 T6766 arg2Of potency,increase
R5139 T6768 T6767 arg1Of potency,the
R5140 T6768 T6769 arg1Of potency,of
R5141 T6768 T6773 arg1Of potency,vivo
R5142 T6771 T6769 arg2Of cytokine,of
R5143 T6771 T6770 arg1Of cytokine,the
R5144 T6773 T6772 arg1Of vivo,in
R5145 T6775 T6774 arg2Of 19,[
R5146 T6776 T6774 arg3Of ],[
R5147 T6673 T6666 arg1Of were,that
R5148 T6673 T6675 arg1Of were,under
R5149 T6674 T6673 arg2Of functional,were
R5150 T6779 T6777 arg2Of mice,In
R5151 T6779 T6778 arg1Of mice,these
R5152 T6782 T6781 arg1Of percentage,the
R5153 T6677 T6675 arg2Of conditions,under
R5154 T6782 T6783 arg1Of percentage,of
R5155 T6782 T6790 arg1Of percentage,decreased
R5156 T6784 T6785 arg1Of CD4+CD25+,and
R5157 T6786 T6785 arg2Of FOXP3+,and
R5158 T6788 T6783 arg2Of cells,of
R5159 T6788 T6784 arg1Of cells,CD4+CD25+
R5160 T6788 T6786 arg1Of cells,FOXP3+
R5161 T6788 T6787 arg1Of cells,T
R5162 T6790 T6777 arg1Of decreased,In
R5163 T6790 T6780 arg1Of decreased,","
R5164 T6790 T6789 arg1Of decreased,dramatically
R5165 T6790 T6791 arg1Of decreased,when
R5166 T6677 T6676 arg1Of conditions,these
R5167 T6677 T6678 arg1Of conditions,(
R5168 T6680 T6678 arg2Of 5C,(
R5169 T6795 T6792 arg1Of complexes,the
R5170 T6795 T6793 arg1Of complexes,antibody-cytokine
R5171 T6795 T6794 arg1Of complexes,immune
R5172 T6795 T6796 arg1Of complexes,were
R5173 T6795 T6797 arg2Of complexes,injected
R5174 T6797 T6791 arg2Of injected,when
R5175 T6797 T6796 arg2Of injected,were
R5176 T6797 T6798 arg1Of injected,(
R5177 T6800 T6798 arg2Of S2,(
R5178 T6800 T6799 arg1Of S2,Figure
R5179 T6801 T6798 arg3Of ),(
R5180 T6803 T6802 arg1Of administration,Upon
R5181 T6680 T6679 arg1Of 5C,Figure
R5182 T6681 T6678 arg3Of ),(
R5183 T6803 T6804 arg1Of administration,of
R5184 T6803 T6821 arg1Of administration,as
R5185 T6682 T6689 arg1Of Increasing,","
R5186 T6682 T6690 arg1Of Increasing,whereas
R5187 T6683 T6682 arg2Of amounts,Increasing
R5188 T6809 T6805 arg1Of complexes,rmIL-4
R5189 T6809 T6806 arg1Of complexes,plus
R5190 T6809 T6807 arg1Of complexes,anti-IL-4
R5191 T6809 T6808 arg1Of complexes,mAb
R5192 T6809 T6810 arg1Of complexes,","
R5193 T6810 T6804 arg2Of ",",of
R5194 T6813 T6810 arg2Of number,","
R5195 T6813 T6811 arg1Of number,the
R5196 T6813 T6812 arg1Of number,total
R5197 T6813 T6814 arg1Of number,of
R5198 T6683 T6684 arg1Of amounts,of
R5199 T6688 T6684 arg2Of GATA3,of
R5200 T6817 T6814 arg2Of cell,of
R5201 T6817 T6815 arg1Of cell,CD4+CD25+
R5202 T6817 T6816 arg1Of cell,T
R5203 T6821 T6818 arg1Of as,","
R5204 T6821 T6819 arg1Of as,as
R5205 T6821 T6820 arg1Of as,well
R5206 T6825 T6821 arg2Of cells,as
R5207 T6825 T6822 arg1Of cells,the
R5208 T6825 T6823 arg1Of cells,Foxp3+
R5209 T6825 T6824 arg1Of cells,T
R5210 T6825 T6826 arg2Of cells,diminished
R5211 T6826 T6835 arg1Of diminished,","
R5212 T6826 T6836 modOf diminished,confirming
R5213 T6828 T6826 arg1Of half,diminished
R5214 T6828 T6827 arg2Of half,by
R5215 T6828 T6829 arg1Of half,(
R5216 T6688 T6685 arg1Of GATA3,IL-4
R5217 T6688 T6686 arg1Of GATA3,increase
R5218 T6831 T6830 arg1Of S2G,Figure
R5219 T6831 T6832 arg1Of S2G,and
R5220 T6688 T6687 arg1Of GATA3,intracellular
R5221 T6832 T6829 arg2Of and,(
R5222 T6691 T6692 arg1Of FOXP3,decreased
R5223 T6833 T6832 arg2Of S2H,and
R5224 T6834 T6829 arg3Of ),(
R5225 T6692 T6690 arg2Of decreased,whereas
R5226 T6692 T6693 arg1Of decreased,","
R5227 T6692 T6694 arg1Of decreased,which
R5228 T6692 T6695 arg1Of decreased,is
R5229 T6840 T6838 arg1Of percentage,the
R5230 T6840 T6839 arg1Of percentage,lower
R5231 T6840 T6841 arg1Of percentage,was
R5232 T6840 T6843 arg1Of percentage,due
R5233 T6841 T6836 arg2Of was,confirming
R5234 T6841 T6837 arg1Of was,that
R5235 T6841 T6842 arg1Of was,not
R5236 T6843 T6841 arg2Of due,was
R5237 T6692 T6696 arg1Of decreased,consistent
R5238 T6696 T6695 arg2Of consistent,is
R5239 T6843 T6844 arg1Of due,to
R5240 T6696 T6697 arg1Of consistent,with
R5241 T6846 T6845 arg1Of increase,an
R5242 T6846 T6847 arg1Of increase,in
R5243 T6846 T6852 arg1Of increase,but
R5244 T6700 T6697 arg2Of analysis,with
R5245 T6850 T6847 arg2Of cells,in
R5246 T6850 T6848 arg1Of cells,the
R5247 T6850 T6849 arg1Of cells,CD4+CD25–
R5248 T6852 T6844 arg2Of but,to
R5249 T6852 T6851 arg1Of but,","
R5250 T6855 T6852 arg2Of decrease,but
R5251 T6855 T6853 arg1Of decrease,a
R5252 T6855 T6854 arg1Of decrease,real
R5253 T6855 T6856 arg1Of decrease,of
R5254 T6700 T6698 arg1Of analysis,the
R5255 T6700 T6699 arg1Of analysis,mRNA
R5256 T6700 T6701 arg1Of analysis,(
R5257 T6859 T6856 arg2Of cells,of
R5258 T6859 T6857 arg1Of cells,CD4+CD25+
R5259 T6859 T6858 arg1Of cells,T
R5260 T6703 T6701 arg2Of 5D,(
R5261 T6861 T6860 arg2Of conclusion,In
R5262 T6863 T6865 arg1Of IL-4,regulates
R5263 T6865 T6860 arg1Of regulates,In
R5264 T6865 T6862 arg1Of regulates,","
R5265 T6865 T6864 arg1Of regulates,negatively
R5266 T6865 T6876 arg1Of regulates,vitro
R5267 T6865 T6880 arg1Of regulates,vivo
R5268 T6867 T6868 arg1Of natural,or
R5269 T6703 T6702 arg1Of 5D,Figure
R5270 T6869 T6868 arg2Of inducible,or
R5271 T6704 T6701 arg3Of ),(
R5272 T6707 T6708 arg1Of injection,of
R5273 T6872 T6865 arg2Of turnover,regulates
R5274 T6872 T6866 arg1Of turnover,the
R5275 T6872 T6867 arg1Of turnover,natural
R5276 T6872 T6869 arg1Of turnover,inducible
R5277 T6872 T6870 arg1Of turnover,Treg
R5278 T6872 T6871 arg1Of turnover,cell
R5279 T6874 T6873 arg1Of only,not
R5280 T6875 T6874 arg1Of in,only
R5281 T6876 T6875 arg1Of vitro,in
R5282 T6876 T6877 arg1Of vitro,but
R5283 T6877 T6878 arg1Of but,also
R5284 T6880 T6877 arg2Of vivo,but
R5285 T6880 T6879 arg1Of vivo,in
R5286 T6707 T6710 arg1Of injection,into
R5287 T6707 T6714 arg1Of injection,decreased
R5288 T6882 T6881 arg1Of study,To
R5289 T6709 T6708 arg2Of IL-4,of
R5290 T6884 T6882 arg2Of effects,study
R5291 T6884 T6883 arg1Of effects,the
R5292 T6884 T6885 arg1Of effects,of
R5293 T6884 T6887 arg1Of effects,on
R5294 T6886 T6885 arg2Of IL-4,of
R5295 T6890 T6891 arg1Of natural,or
R5296 T6713 T6710 arg2Of mice,into
R5297 T6892 T6891 arg2Of inducible,or
R5298 T6894 T6887 arg2Of cells,on
R5299 T6894 T6888 arg1Of cells,already-existing
R5300 T6894 T6889 arg1Of cells,human
R5301 T6894 T6890 arg1Of cells,natural
R5302 T6894 T6892 arg1Of cells,inducible
R5303 T6894 T6893 arg1Of cells,Treg
R5304 T6896 T6882 arg1Of we,study
R5305 T6896 T6897 arg1Of we,exposed
R5306 T6897 T6881 modOf exposed,To
R5307 T6897 T6895 arg1Of exposed,","
R5308 T6897 T6906 arg1Of exposed,to
R5309 T6713 T6711 arg1Of mice,wild-type
R5310 T6713 T6712 arg1Of mice,B6
R5311 T6901 T6897 arg2Of cells,exposed
R5312 T6901 T6898 arg2Of cells,sorted
R5313 T6901 T6899 arg1Of cells,CD25+
R5314 T6901 T6900 arg1Of cells,T
R5315 T6901 T6902 arg1Of cells,(
R5316 T6904 T6902 arg2Of cells,(
R5317 T6904 T6903 arg1Of cells,nTreg
R5318 T6905 T6902 arg3Of ),(
R5319 T6714 T6705 arg1Of decreased,Furthermore
R5320 T6907 T6908 arg1Of IL-4,and
R5321 T6908 T6912 arg1Of and,and
R5322 T6714 T6706 arg1Of decreased,","
R5323 T6714 T6722 arg1Of decreased,vivo
R5324 T6716 T6717 arg1Of inducible,or
R5325 T6911 T6908 arg2Of expression,and
R5326 T6911 T6909 arg2Of expression,analyzed
R5327 T6911 T6910 arg1Of expression,FOXP3
R5328 T6912 T6906 arg2Of and,to
R5329 T6914 T6912 arg2Of capacity,and
R5330 T6914 T6913 arg1Of capacity,suppressive
R5331 T6718 T6717 arg2Of natural,or
R5332 T6918 T6915 arg2Of cells,In
R5333 T6720 T6714 arg2Of number,decreased
R5334 T6918 T6916 arg1Of cells,already-existing
R5335 T6918 T6917 arg1Of cells,Treg
R5336 T6920 T6921 arg1Of IL-4,failed
R5337 T6920 T6923 arg1Of IL-4,inhibit
R5338 T6720 T6715 arg1Of number,the
R5339 T6921 T6915 arg1Of failed,In
R5340 T6921 T6919 arg1Of failed,","
R5341 T6720 T6716 arg1Of number,inducible
R5342 T6921 T6931 arg1Of failed,and
R5343 T6720 T6718 arg1Of number,natural
R5344 T6720 T6719 arg1Of number,Treg
R5345 T6722 T6721 arg1Of vivo,in
R5346 T6923 T6921 arg2Of inhibit,failed
R5347 T6923 T6922 arg1Of inhibit,to
R5348 T6925 T6923 arg2Of expression,inhibit
R5349 T6925 T6924 arg1Of expression,FOXP3
R5350 T6925 T6926 arg1Of expression,(
R5351 T6724 T6723 arg1Of distinction,A
R5352 T6928 T6926 arg2Of S3A,(
R5353 T6928 T6927 arg1Of S3A,Figure
R5354 T6929 T6926 arg3Of ),(
R5355 T6931 T6930 arg1Of and,","
R5356 T6724 T6725 arg1Of distinction,of
R5357 T6724 T6729 arg1Of distinction,is
R5358 T6724 T6731 arg1Of distinction,possible
R5359 T6934 T6932 arg1Of capacity,the
R5360 T6934 T6933 arg1Of capacity,suppressive
R5361 T6934 T6935 arg1Of capacity,was
R5362 T6934 T6937 arg2Of capacity,altered
R5363 T6728 T6725 arg2Of subsets,of
R5364 T6728 T6726 arg1Of subsets,the
R5365 T6937 T6931 arg2Of altered,and
R5366 T6937 T6935 arg2Of altered,was
R5367 T6937 T6936 arg1Of altered,not
R5368 T6937 T6938 arg1Of altered,(
R5369 T6728 T6727 arg1Of subsets,Treg
R5370 T6940 T6938 arg2Of S3C,(
R5371 T6940 T6939 arg1Of S3C,Figure
R5372 T6941 T6938 arg3Of ),(
R5373 T6729 T6730 arg1Of is,not
R5374 T6943 T6942 arg1Of pre-existing,Similarly
R5375 T6729 T6732 arg1Of is,","
R5376 T6729 T6733 arg1Of is,because
R5377 T6945 T6943 arg1Of cells,pre-existing
R5378 T6945 T6944 arg1Of cells,iTreg
R5379 T6945 T6946 arg1Of cells,did
R5380 T6945 T6948 arg1Of cells,decrease
R5381 T6731 T6729 arg2Of possible,is
R5382 T6948 T6946 arg2Of decrease,did
R5383 T6948 T6947 arg1Of decrease,not
R5384 T6948 T6951 arg1Of decrease,upon
R5385 T6950 T6948 arg2Of expression,decrease
R5386 T6950 T6949 arg1Of expression,FOXP3
R5387 T6735 T6734 arg1Of activated,recently
R5388 T6953 T6951 arg2Of exposure,upon
R5389 T6953 T6952 arg1Of exposure,IL-4
R5390 T6953 T6954 arg1Of exposure,(
R5391 T6956 T6954 arg2Of S3B,(
R5392 T6956 T6955 arg1Of S3B,Figure
R5393 T6957 T6954 arg3Of ),(
R5394 T6737 T6735 arg1Of cells,activated
R5395 T6737 T6736 arg1Of cells,iTreg
R5396 T6737 T6739 arg1Of cells,express
R5397 T6739 T6733 arg2Of express,because
R5398 T6739 T6738 arg1Of express,also
R6322 T8353 T8352 arg1Of Role,The
R6323 T8353 T8354 arg1Of Role,of
R6324 T8353 T8356 arg1Of Role,in
R6325 T8355 T8354 arg2Of TGF-β,of
R6326 T8359 T8356 arg2Of Differentiation,in
R6327 T8359 T8357 arg1Of Differentiation,T
R6328 T8359 T8358 arg1Of Differentiation,Cell
R6329 T8362 T8361 arg1Of CD25,TGF-β–reduced
R6330 T8362 T8363 arg1Of CD25,","
R6331 T8363 T8367 arg1Of ",",and
R6332 T8365 T8363 arg2Of expression,","
R6333 T8365 T8364 arg1Of expression,IL-4
R6334 T8367 T8360 arg2Of and,Although
R6335 T8367 T8366 arg1Of and,","
R6336 T8369 T8367 arg2Of expression,and
R6337 T8369 T8368 arg1Of expression,CD25
R6338 T8369 T8370 arg1Of expression,(
R6339 T8372 T8370 arg2Of 6B,(
R6340 T8372 T8371 arg1Of 6B,Figure
R6341 T8373 T8370 arg3Of ),(
R6342 T8375 T8377 arg1Of IL-4,inhibited
R6343 T8377 T8360 arg1Of inhibited,Although
R6344 T8377 T8374 arg1Of inhibited,","
R6345 T8377 T8376 arg1Of inhibited,significantly
R6346 T8377 T8391 arg1Of inhibited,","
R6347 T8377 T8392 arg1Of inhibited,as
R6348 T8379 T8377 arg2Of induction,inhibited
R6349 T8379 T8378 arg1Of induction,TGF-β–mediated
R6350 T8379 T8380 arg1Of induction,of
R6351 T8379 T8382 arg1Of induction,in
R6352 T8381 T8380 arg2Of FOXP3,of
R6353 T8385 T8382 arg2Of cells,in
R6354 T8385 T8383 arg1Of cells,naive
R6355 T8385 T8384 arg1Of cells,T
R6356 T8385 T8386 arg2Of cells,driven
R6357 T8386 T8387 arg1Of driven,toward
R6358 T8390 T8387 arg2Of cells,toward
R6359 T8390 T8388 arg1Of cells,FOXP3+
R6360 T8390 T8389 arg1Of cells,T
R6361 T8393 T8392 arg2Of shown,as
R6362 T8396 T8393 arg1Of analysis,shown
R6363 T8396 T8394 arg2Of analysis,by
R6364 T8396 T8395 arg1Of analysis,FACS
R6365 T8396 T8397 arg1Of analysis,(
R6366 T8399 T8397 arg2Of 6A,(
R6367 T8399 T8398 arg1Of 6A,Figure
R6368 T8400 T8397 arg3Of ),(
R6369 T8401 T8402 arg1Of It,is
R6370 T8401 T8403 arg2Of It,known
R6371 T8403 T8402 arg2Of known,is
R6372 T8405 T8406 arg1Of IL-4,is
R6373 T8405 T8412 arg1Of IL-4,may
R6374 T8405 T8414 arg1Of IL-4,favor
R6375 T8405 T8422 arg1Of IL-4,decrease
R6376 T8406 T8411 arg1Of is,and
R6377 T8410 T8406 arg2Of factor,is
R6378 T8410 T8407 arg1Of factor,a
R6379 T8410 T8408 arg1Of factor,potent
R6380 T8410 T8409 arg1Of factor,growth
R6381 T8411 T8403 arg3Of and,known
R6382 T8411 T8404 arg1Of and,that
R6383 T8414 T8420 arg1Of favor,and
R6384 T8416 T8414 arg2Of proliferation,favor
R6385 T8416 T8415 arg1Of proliferation,the
R6386 T8416 T8417 arg1Of proliferation,of
R6387 T8419 T8417 arg2Of cells,of
R6388 T8419 T8418 arg1Of cells,FOXP3–
R6389 T8420 T8411 arg2Of and,and
R6390 T8420 T8412 arg2Of and,may
R6391 T8420 T8413 arg1Of and,therefore
R6392 T8422 T8420 arg2Of decrease,and
R6393 T8422 T8421 arg1Of decrease,thus
R6394 T8425 T8422 arg2Of percentage,decrease
R6395 T8425 T8423 arg1Of percentage,the
R6396 T8425 T8424 arg1Of percentage,relative
R6397 T8425 T8426 arg1Of percentage,of
R6398 T8428 T8426 arg2Of cells,of
R6399 T8428 T8427 arg1Of cells,FOXP3+
R6400 T8431 T8432 arg1Of analysis,of
R6401 T8431 T8436 arg1Of analysis,by
R6402 T8431 T8438 arg1Of analysis,demonstrated
R6403 T8435 T8432 arg2Of kinetics,of
R6404 T8435 T8433 arg1Of kinetics,cell
R6405 T8435 T8434 arg1Of kinetics,division
R6406 T8437 T8436 arg2Of CFSE-labeling,by
R6407 T8438 T8429 arg1Of demonstrated,However
R6408 T8438 T8430 arg1Of demonstrated,","
R6409 T8440 T8441 arg1Of IL-4,did
R6410 T8440 T8444 arg1Of IL-4,promote
R6411 T8444 T8438 arg2Of promote,demonstrated
R6412 T8444 T8439 arg1Of promote,that
R6413 T8444 T8441 arg2Of promote,did
R6414 T8444 T8442 arg1Of promote,not
R6415 T8444 T8443 arg1Of promote,differentially
R6416 T8444 T8449 arg1Of promote,over
R6417 T8446 T8444 arg2Of growth,promote
R6418 T8446 T8445 arg1Of growth,cell
R6419 T8446 T8447 arg1Of growth,of
R6420 T8448 T8447 arg2Of FOXP3+,of
R6421 T8450 T8449 arg2Of that,over
R6422 T8450 T8451 arg1Of that,of
R6423 T8452 T8451 arg2Of FOXP3–,of
R6424 T8454 T8453 arg2Of fact,In
R6425 T8456 T8455 arg1Of populations,both
R6426 T8456 T8457 arg1Of populations,showed
R6427 T8457 T8453 arg1Of showed,In
R6428 T8459 T8458 arg1Of enhanced,similarly
R6429 T8460 T8457 arg2Of proliferation,showed
R6430 T8460 T8459 arg1Of proliferation,enhanced
R6431 T8460 T8461 arg1Of proliferation,(
R6432 T8463 T8461 arg2Of 6A,(
R6433 T8463 T8462 arg1Of 6A,Figure
R6434 T8464 T8461 arg3Of ),(
R6435 T8468 T8466 arg1Of induction,the
R6436 T8468 T8467 arg1Of induction,TGF-β–mediated
R6437 T8468 T8469 arg1Of induction,of
R6438 T8468 T8472 arg1Of induction,was
R6439 T8468 T8474 arg2Of induction,caused
R6440 T8471 T8469 arg2Of expression,of
R6441 T8471 T8470 arg1Of expression,FOXP3
R6442 T8474 T8465 arg1Of caused,Furthermore
R6443 T8474 T8472 arg2Of caused,was
R6444 T8474 T8473 arg1Of caused,not
R6445 T8474 T8481 arg1Of caused,","
R6446 T8474 T8482 arg1Of caused,since
R6447 T8474 T8496 arg1Of caused,(
R6448 T8474 T8505 arg1Of caused,and
R6449 T8476 T8474 arg1Of overgrowth,caused
R6450 T8476 T8475 arg2Of overgrowth,by
R6451 T8476 T8477 arg1Of overgrowth,of
R6452 T8480 T8477 arg2Of minority,of
R6453 T8480 T8478 arg1Of minority,a
R6454 T8480 T8479 arg1Of minority,CD25–FOXP3+
R6455 T8484 T8483 arg1Of number,the
R6456 T8484 T8485 arg1Of number,of
R6457 T8484 T8488 arg1Of number,was
R6458 T8484 T8489 arg1Of number,low/absent
R6459 T8487 T8485 arg2Of cells,of
R6460 T8487 T8486 arg1Of cells,FOXP3+
R6461 T8488 T8482 arg2Of was,since
R6462 T8488 T8490 arg1Of was,in
R6463 T8489 T8488 arg2Of low/absent,was
R6464 T8495 T8490 arg2Of cells,in
R6465 T8495 T8491 arg1Of cells,the
R6466 T8495 T8492 arg2Of cells,purified
R6467 T8495 T8493 arg1Of cells,CD4+CD45RA+
R6468 T8495 T8494 arg1Of cells,T
R6469 T8499 T8498 arg1Of %,0
R6470 T8499 T8500 arg1Of %,and
R6471 T8501 T8500 arg2Of 1,and
R6472 T8502 T8496 arg2Of %,(
R6473 T8502 T8497 arg1Of %,between
R6474 T8502 T8499 arg1Of %,%
R6475 T8502 T8501 arg1Of %,1
R6476 T8503 T8496 arg3Of ),(
R6477 T8505 T8504 arg1Of and,","
R6478 T8508 T8506 arg1Of cells,the
R6479 T8508 T8507 arg1Of cells,FOXP3+
R6480 T8508 T8509 arg1Of cells,were
R6481 T8508 T8511 arg2Of cells,confined
R6482 T8511 T8505 arg2Of confined,and
R6483 T8511 T8509 arg2Of confined,were
R6484 T8511 T8510 arg1Of confined,not
R6485 T8511 T8512 arg1Of confined,to
R6486 T8516 T8512 arg2Of cells,to
R6487 T8516 T8513 arg1Of cells,the
R6488 T8516 T8514 arg1Of cells,highly
R6489 T8516 T8515 arg2Of cells,divided
R6490 T8517 T8518 arg1Of CD25,was
R6491 T8517 T8519 arg2Of CD25,down-regulated
R6492 T8519 T8518 arg2Of down-regulated,was
R6493 T8519 T8520 arg1Of down-regulated,in
R6494 T8522 T8521 arg1Of cells,TGF-β–treated
R6495 T8522 T8523 arg1Of cells,compare
R6496 T8523 T8518 modOf compare,was
R6497 T8523 T8524 arg1Of compare,to
R6498 T8527 T8524 arg2Of cells,to
R6499 T8527 T8525 arg2Of cells,activated
R6500 T8527 T8526 arg1Of cells,T
R6501 T8527 T8528 arg1Of cells,","
R6502 T8527 T8529 arg1Of cells,which
R6503 T8527 T8530 arg1Of cells,was
R6504 T8527 T8533 arg1Of cells,pronounced
R6505 T8530 T8534 arg1Of was,in
R6506 T8532 T8531 arg1Of more,even
R6507 T8533 T8530 arg2Of pronounced,was
R6508 T8533 T8532 arg1Of pronounced,more
R6509 T8535 T8534 arg2Of cells,in
R6510 T8535 T8536 arg2Of cells,cultured
R6511 T8536 T8537 arg1Of cultured,with
R6512 T8538 T8539 arg1Of TGF-β,and
R6513 T8539 T8537 arg2Of and,with
R6514 T8540 T8539 arg2Of IL-4,and
R6515 T8542 T8541 arg1Of addition,The
R6516 T8542 T8543 arg1Of addition,of
R6517 T8542 T8545 arg1Of addition,to
R6518 T8542 T8548 arg1Of addition,decreased
R6519 T8544 T8543 arg2Of IL-4,of
R6520 T8547 T8545 arg2Of conditions,to
R6521 T8547 T8546 arg1Of conditions,iTreg-driving
R6522 T8550 T8548 arg2Of number,decreased
R6523 T8550 T8549 arg1Of number,the
R6524 T8550 T8551 arg1Of number,of
R6525 T8553 T8551 arg2Of cells,of
R6526 T8553 T8552 arg1Of cells,FOXP3+
R6527 T8553 T8554 arg1Of cells,(
R6528 T8556 T8554 arg2Of 6B,(
R6529 T8556 T8555 arg1Of 6B,Figure
R6530 T8557 T8554 arg3Of ),(
R6531 T8559 T8558 arg2Of line,In
R6532 T8559 T8560 arg1Of line,with
R6533 T8563 T8560 arg2Of findings,with
R6534 T8563 T8561 arg1Of findings,the
R6535 T8563 T8562 arg1Of findings,previous
R6536 T8567 T8565 arg1Of cells,the
R6537 T8567 T8566 arg1Of cells,IL-4–producing
R6538 T8567 T8568 arg1Of cells,and
R6539 T8568 T8573 arg1Of and,are
R6540 T8572 T8568 arg2Of cells,and
R6541 T8572 T8569 arg1Of cells,the
R6542 T8572 T8570 arg1Of cells,FOXP3
R6543 T8572 T8571 arg1Of cells,expressing
R6544 T8573 T8558 arg1Of are,In
R6545 T8573 T8564 arg1Of are,","
R6546 T8575 T8573 arg2Of populations,are
R6547 T8575 T8574 arg1Of populations,nonoverlapping
R6548 T8577 T8578 arg1Of FOXP3,is
R6549 T8577 T8579 arg2Of FOXP3,known
R6550 T8577 T8581 arg1Of FOXP3,act
R6551 T8579 T8576 arg2Of known,Since
R6552 T8579 T8578 arg2Of known,is
R6553 T8581 T8579 arg3Of act,known
R6554 T8581 T8580 arg1Of act,to
R6555 T8581 T8582 arg1Of act,as
R6556 T8584 T8582 arg2Of repressor,as
R6557 T8584 T8583 arg1Of repressor,a
R6558 T8584 T8585 arg1Of repressor,of
R6559 T8587 T8585 arg2Of expression,of
R6560 T8587 T8586 arg1Of expression,cytokine
R6561 T8587 T8588 arg1Of expression,[
R6562 T8589 T8588 arg2Of 20,[
R6563 T8590 T8588 arg3Of ],[
R6564 T8592 T8594 arg1Of we,analyzed
R6565 T8594 T8576 arg1Of analyzed,Since
R6566 T8594 T8591 arg1Of analyzed,","
R6567 T8594 T8593 arg1Of analyzed,therefore
R6568 T8595 T8596 arg1Of GATA3,and
R6569 T8597 T8596 arg2Of FOXP3,and
R6570 T8598 T8594 arg2Of expression,analyzed
R6571 T8598 T8595 arg1Of expression,GATA3
R6572 T8598 T8597 arg1Of expression,FOXP3
R6573 T8600 T8599 arg1Of expression,The
R6574 T8600 T8601 arg1Of expression,kinetic
R6575 T8600 T8611 arg1Of expression,demonstrated
R6576 T8601 T8602 arg1Of kinetic,of
R6577 T8605 T8602 arg2Of cells,of
R6578 T8605 T8603 arg1Of cells,naive
R6579 T8605 T8604 arg1Of cells,T
R6580 T8605 T8606 arg2Of cells,exposed
R6581 T8606 T8607 arg1Of exposed,to
R6582 T8608 T8609 arg1Of IL-4,and
R6583 T8609 T8607 arg2Of and,to
R6584 T8610 T8609 arg2Of TGF-β,and
R6585 T8613 T8614 arg1Of GATA3,and
R6586 T8614 T8616 arg1Of and,are
R6587 T8614 T8618 arg2Of and,found
R6588 T8614 T8629 arg1Of and,co-express
R6589 T8614 T8638 arg1Of and,establishing
R6590 T8615 T8614 arg2Of FOXP3,and
R6591 T8618 T8616 arg2Of found,are
R6592 T8618 T8617 arg1Of found,initially
R6593 T8618 T8619 arg1Of found,in
R6594 T8618 T8627 arg1Of found,but
R6595 T8621 T8619 arg2Of populations,in
R6596 T8621 T8620 arg1Of populations,separate
R6597 T8621 T8622 arg1Of populations,(
R6598 T8623 T8622 arg2Of day,(
R6599 T8623 T8624 arg1Of day,2
R6600 T8625 T8622 arg3Of ),(
R6601 T8627 T8611 arg2Of but,demonstrated
R6602 T8627 T8612 arg1Of but,that
R6603 T8627 T8626 arg1Of but,","
R6604 T8627 T8655 arg1Of but,","
R6605 T8627 T8656 modOf but,suggesting
R6606 T8629 T8627 arg2Of co-express,but
R6607 T8629 T8628 arg1Of co-express,transiently
R6608 T8629 T8636 arg1Of co-express,","
R6609 T8629 T8637 arg1Of co-express,before
R6610 T8631 T8629 arg2Of factors,co-express
R6611 T8631 T8630 arg1Of factors,both
R6612 T8631 T8632 arg1Of factors,(
R6613 T8634 T8632 arg2Of 4–8,(
R6614 T8634 T8633 arg1Of 4–8,days
R6615 T8635 T8632 arg3Of ),(
R6616 T8638 T8637 arg2Of establishing,before
R6617 T8638 T8641 arg1Of establishing,at
R6618 T8640 T8638 arg2Of populations,establishing
R6619 T8640 T8639 arg1Of populations,separate
R6620 T8643 T8641 arg2Of end,at
R6621 T8643 T8642 arg1Of end,the
R6622 T8643 T8644 arg1Of end,of
R6623 T8647 T8644 arg2Of process,of
R6624 T8647 T8645 arg1Of process,the
R6625 T8647 T8646 arg1Of process,differentiation
R6626 T8647 T8648 arg1Of process,(
R6627 T8649 T8650 arg1Of day,10
R6628 T8649 T8651 arg1Of day,;
R6629 T8651 T8648 arg2Of ;,(
R6630 T8653 T8651 arg2Of 6C,;
R6631 T8653 T8652 arg1Of 6C,Figure
R6632 T8654 T8648 arg3Of ),(
R6633 T8658 T8659 arg1Of GATA3,inhibits
R6634 T8658 T8666 arg1Of GATA3,repressing
R6635 T8659 T8656 arg2Of inhibits,suggesting
R6636 T8659 T8657 arg1Of inhibits,that
R6637 T8659 T8665 arg1Of inhibits,by
R6638 T8661 T8659 arg2Of development,inhibits
R6639 T8661 T8660 arg1Of development,the
R6640 T8661 T8662 arg1Of development,of
R6641 T8664 T8662 arg2Of cells,of
R6642 T8664 T8663 arg1Of cells,iTreg
R6643 T8666 T8665 arg2Of repressing,by
R6644 T8667 T8666 arg2Of FOXP3,repressing
R6645 T8669 T8668 arg1Of results,These
R6646 T8669 T8670 arg1Of results,showed
R6647 T8672 T8673 arg1Of IL-4,acts
R6648 T8673 T8670 arg2Of acts,showed
R6649 T8673 T8671 arg1Of acts,that
R6650 T8673 T8675 arg1Of acts,vitro
R6651 T8673 T8676 arg1Of acts,as
R6652 T8673 T8682 arg1Of acts,","
R6653 T8673 T8683 arg1Of acts,without
R6654 T8673 T8700 arg1Of acts,by
R6655 T8675 T8674 arg1Of vitro,in
R6656 T8678 T8676 arg2Of inhibitor,as
R6657 T8678 T8677 arg1Of inhibitor,an
R6658 T8678 T8679 arg1Of inhibitor,of
R6659 T8681 T8679 arg2Of expression,of
R6660 T8681 T8680 arg1Of expression,FOXP3
R6661 T8683 T8698 arg1Of without,and
R6662 T8684 T8683 arg2Of interfering,without
R6663 T8684 T8685 arg1Of interfering,with
R6664 T8684 T8688 arg1Of interfering,","
R6665 T8684 T8690 modOf interfering,acting
R6666 T8687 T8685 arg2Of signaling,with
R6667 T8687 T8686 arg1Of signaling,TGF-β
R6668 T8690 T8689 arg1Of acting,probably
R6669 T8690 T8691 arg1Of acting,at
R6670 T8693 T8691 arg2Of level,at
R6671 T8693 T8692 arg1Of level,the
R6672 T8693 T8694 arg1Of level,of
R6673 T8696 T8694 arg2Of factors,of
R6674 T8696 T8695 arg1Of factors,transcription
R6675 T8698 T8697 arg1Of and,","
R6676 T8700 T8698 arg2Of by,and
R6677 T8700 T8699 arg1Of by,possibly
R6678 T8703 T8700 arg2Of mechanism,by
R6679 T8703 T8701 arg1Of mechanism,a
R6680 T8703 T8702 arg1Of mechanism,GATA3-dependent
R7423 T9866 T9867 arg1Of GATA3,Is
R7424 T9870 T9867 arg2Of Regulator,Is
R7425 T9870 T9868 arg1Of Regulator,a
R7426 T9870 T9869 arg1Of Regulator,Negative
R7427 T9870 T9871 arg1Of Regulator,of
R7428 T9873 T9871 arg2Of Expression,of
R7429 T9873 T9872 arg1Of Expression,FOXP3
R7430 T9875 T9874 arg1Of expression,FOXP3
R7431 T9875 T9876 arg1Of expression,decreased
R7432 T9876 T9877 arg1Of decreased,once
R7433 T9876 T9882 arg1Of decreased,;
R7434 T9879 T9878 arg1Of expression,GATA3
R7435 T9879 T9880 arg1Of expression,is
R7436 T9879 T9881 arg1Of expression,high
R7437 T9880 T9877 arg2Of is,once
R7438 T9881 T9880 arg2Of high,is
R7439 T9885 T9886 arg1Of we,hypothesized
R7440 T9886 T9882 arg2Of hypothesized,;
R7441 T9886 T9883 arg1Of hypothesized,therefore
R7442 T9886 T9884 arg1Of hypothesized,","
R7443 T9889 T9886 arg2Of role,hypothesized
R7444 T9889 T9887 arg1Of role,a
R7445 T9889 T9888 arg1Of role,potential
R7446 T9889 T9890 arg1Of role,for
R7447 T9889 T9892 arg1Of role,in
R7448 T9891 T9890 arg2Of GATA3,for
R7449 T9893 T9892 arg2Of repressing,in
R7450 T9894 T9893 arg2Of FOXP3,repressing
R7451 T9898 T9897 arg1Of positive,well-known
R7452 T9899 T9895 arg2Of effect,Besides
R7453 T9899 T9896 arg1Of effect,GATA3′s
R7454 T9899 T9898 arg1Of effect,positive
R7455 T9899 T9900 arg1Of effect,on
R7456 T9902 T9900 arg2Of regulation,on
R7457 T9902 T9901 arg1Of regulation,gene
R7458 T9906 T9904 arg1Of capabilities,GATA3′s
R7459 T9906 T9905 arg1Of capabilities,repressive
R7460 T9906 T9907 arg1Of capabilities,were
R7461 T9906 T9909 arg2Of capabilities,shown
R7462 T9906 T9911 arg1Of capabilities,restrict
R7463 T9906 T9915 arg1Of capabilities,inhibiting
R7464 T9909 T9907 arg2Of shown,were
R7465 T9909 T9908 arg1Of shown,previously
R7466 T9909 T9922 arg1Of shown,and
R7467 T9911 T9909 arg3Of restrict,shown
R7468 T9911 T9910 arg1Of restrict,to
R7469 T9911 T9914 arg1Of restrict,by
R7470 T9913 T9911 arg2Of commitment,restrict
R7471 T9913 T9912 arg1Of commitment,Th1
R7472 T9915 T9914 arg2Of inhibiting,by
R7473 T9917 T9915 arg2Of expression,inhibiting
R7474 T9917 T9916 arg1Of expression,STAT4
R7475 T9917 T9918 arg1Of expression,[
R7476 T9919 T9918 arg2Of "2,21",[
R7477 T9920 T9918 arg3Of ],[
R7478 T9922 T9895 arg1Of and,Besides
R7479 T9922 T9903 arg1Of and,","
R7480 T9922 T9921 arg1Of and,","
R7481 T9924 T9923 arg1Of GATA3,therefore
R7482 T9924 T9925 arg1Of GATA3,prevents
R7483 T9925 T9922 arg2Of prevents,and
R7484 T9926 T9925 arg2Of differentiation,prevents
R7485 T9926 T9927 arg1Of differentiation,into
R7486 T9929 T9927 arg2Of cells,into
R7487 T9929 T9928 arg1Of cells,Th1
R7488 T9931 T9930 arg1Of investigate,To
R7489 T9933 T9934 arg1Of GATA3,can
R7490 T9933 T9936 arg1Of GATA3,inhibit
R7491 T9936 T9931 arg2Of inhibit,investigate
R7492 T9936 T9932 arg1Of inhibit,whether
R7493 T9936 T9934 arg2Of inhibit,can
R7494 T9936 T9935 arg1Of inhibit,directly
R7495 T9938 T9936 arg2Of induction,inhibit
R7496 T9938 T9937 arg1Of induction,FOXP3
R7497 T9940 T9931 arg1Of we,investigate
R7498 T9940 T9941 arg1Of we,transduced
R7499 T9941 T9930 modOf transduced,To
R7500 T9941 T9939 arg1Of transduced,","
R7501 T9941 T9951 arg1Of transduced,in
R7502 T9942 T9943 arg1Of GATA3,or
R7503 T9943 T9941 arg2Of or,transduced
R7504 T9946 T9943 arg2Of GATA3,or
R7505 T9946 T9944 arg1Of GATA3,a
R7506 T9946 T9945 arg2Of GATA3,truncated
R7507 T9946 T9947 arg1Of GATA3,lacking
R7508 T9950 T9947 arg2Of domain,lacking
R7509 T9950 T9948 arg1Of domain,the
R7510 T9950 T9949 arg1Of domain,DNA-binding
R7511 T9956 T9951 arg2Of cells,in
R7512 T9956 T9952 arg1Of cells,human
R7513 T9956 T9953 arg1Of cells,primary
R7514 T9956 T9954 arg1Of cells,CD4+CD45RA+
R7515 T9956 T9955 arg1Of cells,T
R7516 T9956 T9957 arg1Of cells,using
R7517 T9959 T9960 arg1Of TAT-fused,","
R7518 T9962 T9960 arg2Of expressed,","
R7519 T9962 T9961 arg1Of expressed,recombinantly
R7520 T9963 T9957 arg2Of GATA3,using
R7521 T9963 T9958 arg1Of GATA3,a
R7522 T9963 T9959 arg1Of GATA3,TAT-fused
R7523 T9963 T9962 arg1Of GATA3,expressed
R7524 T9965 T9964 arg2Of transduction,After
R7525 T9968 T9967 arg1Of cells,the
R7526 T9968 T9969 arg1Of cells,were
R7527 T9968 T9970 arg2Of cells,activated
R7528 T9970 T9964 arg1Of activated,After
R7529 T9970 T9966 arg1Of activated,","
R7530 T9970 T9969 arg2Of activated,were
R7531 T9970 T9971 arg1Of activated,with
R7532 T9970 T9974 arg1Of activated,in
R7533 T9973 T9971 arg2Of anti-CD3/CD28,with
R7534 T9973 T9972 arg1Of anti-CD3/CD28,soluble
R7535 T9976 T9977 arg1Of presence,or
R7536 T9977 T9974 arg2Of or,in
R7537 T9977 T9975 arg1Of or,the
R7538 T9977 T9979 arg1Of or,of
R7539 T9978 T9977 arg2Of absence,or
R7540 T9980 T9979 arg2Of TGF-β,of
R7541 T9981 T9982 arg1Of TAT-GATA3,was
R7542 T9981 T9984 arg2Of TAT-GATA3,transduced
R7543 T9984 T9982 arg2Of transduced,was
R7544 T9984 T9983 arg1Of transduced,successfully
R7545 T9984 T9985 arg1Of transduced,in
R7546 T9984 T9991 arg1Of transduced,into
R7547 T9987 T9988 arg1Of homogeneous,and
R7548 T9989 T9988 arg2Of dose-dependent,and
R7549 T9990 T9985 arg2Of manner,in
R7550 T9990 T9986 arg1Of manner,a
R7551 T9990 T9987 arg1Of manner,homogeneous
R7552 T9990 T9989 arg1Of manner,dose-dependent
R7553 T9995 T9991 arg2Of cells,into
R7554 T9995 T9992 arg1Of cells,human
R7555 T9995 T9993 arg1Of cells,CD4+
R7556 T9995 T9994 arg1Of cells,T
R7557 T9995 T9996 arg1Of cells,(
R7558 T9998 T9996 arg2Of 7A,(
R7559 T9998 T9997 arg1Of 7A,Figure
R7560 T9998 T9999 arg1Of 7A,","
R7561 T10001 T9999 arg2Of panel,","
R7562 T10001 T10000 arg1Of panel,upper
R7563 T10002 T9996 arg3Of ),(
R7564 T10003 T10004 arg1Of TAT-GATA3,reduced
R7565 T10004 T10007 arg1Of reduced,in
R7566 T10004 T10011 arg1Of reduced,","
R7567 T10004 T10012 arg1Of reduced,whereas
R7568 T10006 T10004 arg2Of expression,reduced
R7569 T10006 T10005 arg1Of expression,FOXP3
R7570 T10010 T10007 arg2Of manner,in
R7571 T10010 T10008 arg1Of manner,a
R7572 T10010 T10009 arg1Of manner,dose-dependent
R7573 T10017 T10013 arg1Of version,a
R7574 T10017 T10014 arg1Of version,DNA-binding
R7575 T10017 T10015 arg1Of version,domain
R7576 T10017 T10016 arg2Of version,truncated
R7577 T10017 T10018 arg1Of version,(
R7578 T10017 T10021 arg1Of version,did
R7579 T10017 T10023 arg1Of version,affect
R7580 T10019 T10018 arg2Of TAT-ΔDBD-GATA3,(
R7581 T10020 T10018 arg3Of ),(
R7582 T10023 T10012 arg2Of affect,whereas
R7583 T10023 T10021 arg2Of affect,did
R7584 T10023 T10022 arg1Of affect,not
R7585 T10023 T10026 arg1Of affect,as
R7586 T10025 T10023 arg2Of expression,affect
R7587 T10025 T10024 arg1Of expression,FOXP3
R7588 T10027 T10026 arg2Of compared,as
R7589 T10027 T10028 arg1Of compared,with
R7590 T10029 T10028 arg2Of expression,with
R7591 T10029 T10030 arg1Of expression,in
R7592 T10032 T10030 arg2Of cells,in
R7593 T10032 T10031 arg1Of cells,untransduced
R7594 T10032 T10033 arg1Of cells,(
R7595 T10035 T10033 arg2Of 7A,(
R7596 T10035 T10034 arg1Of 7A,Figure
R7597 T10036 T10033 arg3Of ),(
R7598 T10038 T10037 arg2Of addition,In
R7599 T10040 T10041 arg1Of we,analyzed
R7600 T10040 T10055 arg1Of we,overexpressing
R7601 T10041 T10037 arg1Of analyzed,In
R7602 T10041 T10039 arg1Of analyzed,","
R7603 T10041 T10053 arg1Of analyzed,","
R7604 T10041 T10055 modOf analyzed,overexpressing
R7605 T10044 T10041 arg2Of effect,analyzed
R7606 T10044 T10042 arg1Of effect,the
R7607 T10044 T10043 arg1Of effect,inhibitory
R7608 T10044 T10045 arg1Of effect,of
R7609 T10044 T10047 arg1Of effect,on
R7610 T10046 T10045 arg2Of GATA3,of
R7611 T10048 T10047 arg2Of FOXP3,on
R7612 T10048 T10049 arg1Of FOXP3,in
R7613 T10052 T10049 arg2Of mice,in
R7614 T10052 T10050 arg1Of mice,transgenic
R7615 T10052 T10051 arg1Of mice,DO11.10
R7616 T10055 T10054 arg1Of overexpressing,constitutively
R7617 T10055 T10057 arg1Of overexpressing,under
R7618 T10056 T10055 arg2Of GATA3,overexpressing
R7619 T10059 T10057 arg2Of control,under
R7620 T10059 T10058 arg1Of control,the
R7621 T10059 T10060 arg1Of control,of
R7622 T10065 T10060 arg2Of region,of
R7623 T10065 T10061 arg1Of region,the
R7624 T10065 T10062 arg1Of region,CD2
R7625 T10065 T10063 arg1Of region,locus
R7626 T10065 T10064 arg1Of region,control
R7627 T10065 T10066 arg1Of region,(
R7628 T10067 T10066 arg2Of DO11.10xCD2-GATA3,(
R7629 T10068 T10066 arg3Of ),(
R7630 T10071 T10069 arg1Of selection,The
R7631 T10071 T10070 arg1Of selection,thymic
R7632 T10071 T10072 arg1Of selection,into
R7633 T10071 T10076 arg1Of selection,is
R7634 T10071 T10078 arg1Of selection,intact
R7635 T10075 T10072 arg2Of lineage,into
R7636 T10075 T10073 arg1Of lineage,the
R7637 T10075 T10074 arg1Of lineage,CD4
R7638 T10076 T10081 arg1Of is,(
R7639 T10078 T10076 arg2Of intact,is
R7640 T10078 T10077 arg1Of intact,largely
R7641 T10078 T10079 arg1Of intact,in
R7642 T10080 T10079 arg2Of DO11.10xCD2-GATA3,in
R7643 T10083 T10081 arg2Of Hendriks,(
R7644 T10083 T10082 arg1Of Hendriks,RW
R7645 T10083 T10084 arg1Of Hendriks,","
R7646 T10086 T10084 arg2Of data,","
R7647 T10086 T10085 arg1Of data,unpublished
R7648 T10087 T10081 arg3Of ),(
R7649 T10089 T10088 arg1Of mice,These
R7650 T10089 T10090 arg1Of mice,develop
R7651 T10090 T10092 arg1Of develop,at
R7652 T10090 T10097 arg1Of develop,but
R7653 T10091 T10090 arg2Of lymphomas,develop
R7654 T10095 T10092 arg2Of age,at
R7655 T10095 T10093 arg1Of age,an
R7656 T10095 T10094 arg1Of age,older
R7657 T10097 T10096 arg1Of but,","
R7658 T10098 T10099 arg1Of signs,of
R7659 T10098 T10102 arg1Of signs,were
R7660 T10098 T10104 arg2Of signs,described
R7661 T10101 T10099 arg2Of disease,of
R7662 T10101 T10100 arg1Of disease,autoimmune
R7663 T10104 T10097 arg2Of described,but
R7664 T10104 T10102 arg2Of described,were
R7665 T10104 T10103 arg1Of described,not
R7666 T10104 T10105 arg1Of described,[
R7667 T10106 T10105 arg2Of 22,[
R7668 T10107 T10105 arg3Of ],[
R7669 T10109 T10108 arg1Of investigate,To
R7670 T10111 T10109 arg2Of effect,investigate
R7671 T10111 T10110 arg1Of effect,the
R7672 T10111 T10112 arg1Of effect,of
R7673 T10111 T10114 arg1Of effect,on
R7674 T10113 T10112 arg2Of GATA3,of
R7675 T10115 T10114 arg2Of iTreg,on
R7676 T10118 T10109 arg1Of cells,investigate
R7677 T10118 T10117 arg1Of cells,CD4+CD62L+CD25–
R7678 T10118 T10119 arg1Of cells,were
R7679 T10118 T10120 arg2Of cells,isolated
R7680 T10118 T10122 arg2Of cells,activated
R7681 T10120 T10121 arg1Of isolated,","
R7682 T10121 T10119 arg2Of ",",were
R7683 T10121 T10133 arg1Of ",",and
R7684 T10122 T10121 arg2Of activated,","
R7685 T10122 T10123 arg1Of activated,with
R7686 T10122 T10125 arg1Of activated,in
R7687 T10124 T10123 arg2Of OVA,with
R7688 T10127 T10128 arg1Of presence,or
R7689 T10128 T10125 arg2Of or,in
R7690 T10128 T10126 arg1Of or,the
R7691 T10128 T10130 arg1Of or,of
R7692 T10129 T10128 arg2Of absence,or
R7693 T10131 T10130 arg2Of TGF-β,of
R7694 T10133 T10108 modOf and,To
R7695 T10133 T10116 arg1Of and,","
R7696 T10133 T10132 arg1Of and,","
R7697 T10135 T10134 arg1Of expression,Foxp3
R7698 T10135 T10136 arg1Of expression,was
R7699 T10135 T10137 arg2Of expression,analyzed
R7700 T10137 T10133 arg2Of analyzed,and
R7701 T10137 T10136 arg2Of analyzed,was
R7702 T10137 T10138 arg1Of analyzed,after
R7703 T10140 T10138 arg2Of d,after
R7704 T10140 T10139 arg1Of d,4
R7705 T10144 T10141 arg1Of cells,The
R7706 T10144 T10142 arg1Of cells,naive
R7707 T10144 T10143 arg1Of cells,CD4+CD25–
R7708 T10144 T10145 arg1Of cells,were
R7709 T10146 T10145 arg2Of Foxp3–,were
R7710 T10146 T10147 arg1Of Foxp3–,(
R7711 T10149 T10147 arg2Of data,(
R7712 T10149 T10148 arg1Of data,unpublished
R7713 T10150 T10147 arg3Of ),(
R7714 T10152 T10151 arg2Of described,As
R7715 T10152 T10153 arg1Of described,for
R7716 T10156 T10153 arg2Of cells,for
R7717 T10156 T10154 arg1Of cells,the
R7718 T10156 T10155 arg1Of cells,human
R7719 T10158 T10160 arg1Of TGF-β,up-regulated
R7720 T10160 T10151 arg1Of up-regulated,As
R7721 T10160 T10157 arg1Of up-regulated,","
R7722 T10160 T10159 arg1Of up-regulated,dramatically
R7723 T10160 T10162 arg1Of up-regulated,in
R7724 T10161 T10160 arg2Of Foxp3,up-regulated
R7725 T10167 T10162 arg2Of mice,in
R7726 T10167 T10163 arg1Of mice,the
R7727 T10167 T10164 arg1Of mice,DO11.10
R7728 T10167 T10165 arg1Of mice,littermate
R7729 T10167 T10166 arg1Of mice,control
R7730 T10169 T10168 arg2Of contrast,In
R7731 T10171 T10172 arg1Of cells,from
R7732 T10171 T10176 arg1Of cells,showed
R7733 T10175 T10172 arg2Of mice,from
R7734 T10175 T10173 arg1Of mice,the
R7735 T10175 T10174 arg1Of mice,CD2-GATA3xDO11.10
R7736 T10176 T10168 arg1Of showed,In
R7737 T10176 T10170 arg1Of showed,","
R7738 T10176 T10181 arg1Of showed,when
R7739 T10178 T10177 arg1Of reduced,dramatically
R7740 T10180 T10176 arg2Of expression,showed
R7741 T10180 T10178 arg1Of expression,reduced
R7742 T10180 T10179 arg1Of expression,Foxp3
R7743 T10182 T10181 arg2Of activated,when
R7744 T10182 T10183 arg1Of activated,with
R7745 T10184 T10185 arg1Of TGF-β,and
R7746 T10185 T10183 arg2Of and,with
R7747 T10185 T10187 arg1Of and,(
R7748 T10186 T10185 arg2Of OVA,and
R7749 T10189 T10187 arg2Of 7B,(
R7750 T10189 T10188 arg1Of 7B,Figure
R7751 T10190 T10187 arg3Of ),(
R7752 T10192 T10191 arg1Of mice,All
R7753 T10192 T10193 arg1Of mice,produced
R7754 T10193 T10198 arg1Of produced,;
R7755 T10195 T10193 arg2Of amounts,produced
R7756 T10195 T10194 arg1Of amounts,similar
R7757 T10195 T10196 arg1Of amounts,of
R7758 T10197 T10196 arg2Of TGF-β,of
R7759 T10200 T10199 arg2Of addition,in
R7760 T10202 T10203 arg1Of Smad7,was
R7761 T10202 T10205 arg2Of Smad7,expressed
R7762 T10205 T10198 arg2Of expressed,;
R7763 T10205 T10199 arg1Of expressed,in
R7764 T10205 T10201 arg1Of expressed,","
R7765 T10205 T10203 arg2Of expressed,was
R7766 T10205 T10204 arg1Of expressed,equally
R7767 T10205 T10206 arg1Of expressed,[
R7768 T10205 T10209 arg1Of expressed,in
R7769 T10205 T10220 arg1Of expressed,","
R7770 T10205 T10221 modOf expressed,indicating
R7771 T10207 T10206 arg2Of 23,[
R7772 T10208 T10206 arg3Of ],[
R7773 T10211 T10209 arg2Of cells,in
R7774 T10211 T10210 arg1Of cells,T
R7775 T10211 T10212 arg1Of cells,of
R7776 T10215 T10212 arg2Of strains,of
R7777 T10215 T10213 arg1Of strains,both
R7778 T10215 T10214 arg1Of strains,mice
R7779 T10215 T10216 arg1Of strains,(
R7780 T10218 T10216 arg2Of 7C,(
R7781 T10218 T10217 arg1Of 7C,Figure
R7782 T10219 T10216 arg3Of ),(
R7783 T10224 T10221 arg2Of signaling,indicating
R7784 T10224 T10222 arg1Of signaling,intact
R7785 T10224 T10223 arg1Of signaling,TGF-β
R7786 T10225 T10226 arg1Of Taken,together
R7787 T10229 T10228 arg1Of results,these
R7788 T10229 T10230 arg1Of results,demonstrated
R7789 T10230 T10225 modOf demonstrated,Taken
R7790 T10230 T10227 arg1Of demonstrated,","
R7791 T10233 T10230 arg2Of role,demonstrated
R7792 T10233 T10231 arg1Of role,a
R7793 T10233 T10232 arg1Of role,repressive
R7794 T10233 T10234 arg1Of role,of
R7795 T10233 T10239 arg1Of role,in
R7796 T10238 T10234 arg2Of factor,of
R7797 T10238 T10235 arg1Of factor,IL-4-induced
R7798 T10238 T10236 arg1Of factor,GATA3
R7799 T10238 T10237 arg1Of factor,transcription
R7800 T10241 T10239 arg2Of generation,in
R7801 T10241 T10240 arg1Of generation,the
R7802 T10241 T10242 arg1Of generation,of
R7803 T10244 T10242 arg2Of cells,of
R7804 T10244 T10243 arg1Of cells,iTreg
R8620 T11599 T11600 arg1Of GATA3,Represses
R8621 T11603 T11600 arg2Of Promoter,Represses
R8622 T11603 T11601 arg1Of Promoter,the
R8623 T11603 T11602 arg1Of Promoter,FOXP3
R8624 T11605 T11604 arg1Of investigate,To
R8625 T11608 T11605 arg2Of mechanism,investigate
R8626 T11608 T11606 arg1Of mechanism,the
R8627 T11608 T11607 arg1Of mechanism,molecular
R8628 T11608 T11609 arg1Of mechanism,of
R8629 T11611 T11609 arg2Of repression,of
R8630 T11611 T11610 arg1Of repression,GATA3-mediated
R8631 T11611 T11612 arg1Of repression,of
R8632 T11614 T11612 arg2Of FOXP3,of
R8633 T11614 T11613 arg1Of FOXP3,human
R8634 T11619 T11616 arg1Of promoter,the
R8635 T11619 T11617 arg1Of promoter,human
R8636 T11619 T11618 arg1Of promoter,FOXP3
R8637 T11619 T11620 arg1Of promoter,was
R8638 T11619 T11621 arg2Of promoter,studied
R8639 T11621 T11620 arg2Of studied,was
R8640 T11621 T11622 arg1Of studied,and
R8641 T11622 T11604 modOf and,To
R8642 T11622 T11615 arg1Of and,","
R8643 T11626 T11623 arg1Of site,a
R8644 T11626 T11624 arg1Of site,palindromic
R8645 T11626 T11625 arg1Of site,binding
R8646 T11626 T11627 arg1Of site,for
R8647 T11626 T11629 arg1Of site,was
R8648 T11626 T11630 arg2Of site,discovered
R8649 T11628 T11627 arg2Of GATA3,for
R8650 T11630 T11622 arg2Of discovered,and
R8651 T11630 T11629 arg2Of discovered,was
R8652 T11633 T11631 arg1Of site,The
R8653 T11633 T11632 arg1Of site,GATA-binding
R8654 T11633 T11634 arg1Of site,is
R8655 T11633 T11635 arg1Of site,located
R8656 T11634 T11647 arg1Of is,[
R8657 T11635 T11634 arg2Of located,is
R8658 T11635 T11638 arg1Of located,upstream
R8659 T11637 T11636 arg1Of bp,303
R8660 T11638 T11637 arg1Of upstream,bp
R8661 T11638 T11639 arg1Of upstream,from
R8662 T11643 T11639 arg2Of site,from
R8663 T11643 T11640 arg1Of site,the
R8664 T11643 T11641 arg1Of site,transcription
R8665 T11643 T11642 arg1Of site,start
R8666 T11643 T11644 arg1Of site,(
R8667 T11645 T11644 arg2Of TSS,(
R8668 T11646 T11644 arg3Of ),(
R8669 T11648 T11647 arg2Of 24,[
R8670 T11649 T11647 arg3Of ],[
R8671 T11651 T11650 arg1Of site,This
R8672 T11651 T11652 arg1Of site,is
R8673 T11651 T11654 arg2Of site,conserved
R8674 T11651 T11667 arg1Of site,may
R8675 T11651 T11669 arg1Of site,play
R8676 T11654 T11652 arg2Of conserved,is
R8677 T11654 T11653 arg1Of conserved,highly
R8678 T11654 T11655 arg1Of conserved,between
R8679 T11654 T11666 arg1Of conserved,and
R8680 T11656 T11657 arg1Of humans,","
R8681 T11657 T11660 arg1Of ",",and
R8682 T11658 T11657 arg2Of mice,","
R8683 T11660 T11655 arg2Of and,between
R8684 T11660 T11659 arg1Of and,","
R8685 T11661 T11660 arg2Of rats,and
R8686 T11661 T11662 arg1Of rats,(
R8687 T11664 T11662 arg2Of S4,(
R8688 T11664 T11663 arg1Of S4,Figure
R8689 T11665 T11662 arg3Of ),(
R8690 T11669 T11666 arg2Of play,and
R8691 T11669 T11667 arg2Of play,may
R8692 T11669 T11668 arg1Of play,therefore
R8693 T11669 T11673 arg1Of play,in
R8694 T11672 T11669 arg2Of role,play
R8695 T11672 T11670 arg1Of role,an
R8696 T11672 T11671 arg1Of role,important
R8697 T11675 T11673 arg2Of regulation,in
R8698 T11675 T11674 arg1Of regulation,FOXP3
R8699 T11678 T11676 arg1Of relevance,The
R8700 T11678 T11677 arg1Of relevance,functional
R8701 T11678 T11679 arg1Of relevance,of
R8702 T11678 T11682 arg1Of relevance,was
R8703 T11678 T11683 arg2Of relevance,studied
R8704 T11681 T11679 arg2Of site,of
R8705 T11681 T11680 arg1Of site,this
R8706 T11683 T11682 arg2Of studied,was
R8707 T11684 T11683 arg3Of using,studied
R8708 T11684 T11688 arg1Of using,[
R8709 T11687 T11684 arg2Of construct,using
R8710 T11687 T11685 arg1Of construct,a
R8711 T11687 T11686 arg1Of construct,FOXP3-promoter
R8712 T11689 T11688 arg2Of 24,[
R8713 T11690 T11688 arg3Of ],[
R8714 T11691 T11692 arg1Of We,transfected
R8715 T11692 T11722 arg1Of transfected,and
R8716 T11697 T11693 arg1Of cells,human
R8717 T11697 T11694 arg1Of cells,primary
R8718 T11697 T11695 arg1Of cells,CD4+
R8719 T11697 T11696 arg1Of cells,T
R8720 T11697 T11698 arg1Of cells,","
R8721 T11698 T11705 arg1Of ",",and
R8722 T11700 T11699 arg1Of vitro,in
R8723 T11703 T11698 arg2Of cells,","
R8724 T11703 T11700 arg1Of cells,vitro
R8725 T11703 T11701 arg2Of cells,differentiated
R8726 T11703 T11702 arg1Of cells,Th2
R8727 T11705 T11692 arg2Of and,transfected
R8728 T11705 T11704 arg1Of and,","
R8729 T11707 T11705 arg2Of cells,and
R8730 T11707 T11706 arg1Of cells,Jurkat
R8731 T11707 T11708 arg1Of cells,(
R8732 T11710 T11709 arg1Of cells,Jurkat
R8733 T11710 T11711 arg1Of cells,are
R8734 T11710 T11712 arg2Of cells,known
R8735 T11710 T11715 arg1Of cells,express
R8736 T11712 T11708 arg2Of known,(
R8737 T11712 T11711 arg2Of known,are
R8738 T11715 T11712 arg3Of express,known
R8739 T11715 T11713 arg1Of express,to
R8740 T11715 T11714 arg1Of express,constitutively
R8741 T11715 T11717 arg1Of express,[
R8742 T11716 T11715 arg2Of GATA3,express
R8743 T11718 T11717 arg2Of "25,26",[
R8744 T11719 T11717 arg3Of ],[
R8745 T11720 T11708 arg3Of ),(
R8746 T11722 T11721 arg1Of and,","
R8747 T11723 T11724 arg1Of we,measured
R8748 T11724 T11722 arg2Of measured,and
R8749 T11727 T11724 arg2Of activity,measured
R8750 T11727 T11725 arg1Of activity,FOXP3
R8751 T11727 T11726 arg1Of activity,promoter
R8752 T11729 T11728 arg1Of promoter,The
R8753 T11729 T11730 arg1Of promoter,was
R8754 T11729 T11732 arg1Of promoter,active
R8755 T11730 T11731 arg1Of was,not
R8756 T11730 T11733 arg1Of was,in
R8757 T11730 T11740 arg1Of was,in
R8758 T11730 T11747 arg1Of was,whereas
R8759 T11732 T11730 arg2Of active,was
R8760 T11733 T11739 arg1Of in,or
R8761 T11738 T11733 arg2Of Jurkat,in
R8762 T11738 T11734 arg1Of Jurkat,the
R8763 T11738 T11735 arg1Of Jurkat,GATA3-expressing
R8764 T11738 T11736 arg1Of Jurkat,cell
R8765 T11738 T11737 arg1Of Jurkat,line
R8766 T11740 T11739 arg2Of in,or
R8767 T11742 T11741 arg1Of in,the
R8768 T11745 T11740 arg2Of cells,in
R8769 T11745 T11742 arg1Of cells,in
R8770 T11745 T11743 arg1Of cells,vitro-differentiated
R8771 T11745 T11744 arg1Of cells,Th2
R8772 T11747 T11746 arg1Of whereas,","
R8773 T11749 T11748 arg1Of construct,the
R8774 T11749 T11750 arg1Of construct,was
R8775 T11749 T11751 arg1Of construct,active
R8776 T11750 T11747 arg2Of was,whereas
R8777 T11751 T11750 arg2Of active,was
R8778 T11751 T11752 arg1Of active,in
R8779 T11755 T11752 arg2Of cells,in
R8780 T11755 T11753 arg1Of cells,the
R8781 T11755 T11754 arg1Of cells,CD4
R8782 T11755 T11756 arg1Of cells,","
R8783 T11755 T11757 arg1Of cells,which
R8784 T11755 T11758 arg1Of cells,express
R8785 T11761 T11758 arg2Of amount,express
R8786 T11761 T11759 arg1Of amount,a
R8787 T11761 T11760 arg1Of amount,lower
R8788 T11761 T11762 arg1Of amount,of
R8789 T11763 T11762 arg2Of GATA3,of
R8790 T11763 T11764 arg1Of GATA3,(
R8791 T11766 T11764 arg2Of 8A,(
R8792 T11766 T11765 arg1Of 8A,Figure
R8793 T11767 T11764 arg3Of ),(
R8794 T11768 T11769 arg1Of Overexpression,of
R8795 T11768 T11771 arg1Of Overexpression,in
R8796 T11768 T11775 arg1Of Overexpression,diminished
R8797 T11770 T11769 arg2Of GATA3,of
R8798 T11774 T11771 arg2Of cells,in
R8799 T11774 T11772 arg1Of cells,naive
R8800 T11774 T11773 arg1Of cells,T
R8801 T11775 T11782 arg1Of diminished,compared
R8802 T11777 T11775 arg2Of activity,diminished
R8803 T11777 T11776 arg1Of activity,luciferase
R8804 T11777 T11778 arg1Of activity,of
R8805 T11781 T11778 arg2Of promoter,of
R8806 T11781 T11779 arg1Of promoter,the
R8807 T11781 T11780 arg1Of promoter,FOXP3
R8808 T11783 T11782 arg2Of with,compared
R8809 T11786 T11783 arg2Of vector,with
R8810 T11786 T11784 arg1Of vector,the
R8811 T11786 T11785 arg1Of vector,control
R8812 T11786 T11787 arg1Of vector,(
R8813 T11789 T11787 arg2Of 8B,(
R8814 T11789 T11788 arg1Of 8B,Figure
R8815 T11790 T11787 arg3Of ),(
R8816 T11793 T11791 arg1Of address,To
R8817 T11793 T11792 arg1Of address,further
R8818 T11795 T11793 arg2Of function,address
R8819 T11795 T11794 arg1Of function,the
R8820 T11795 T11796 arg1Of function,of
R8821 T11799 T11796 arg2Of site,of
R8822 T11799 T11797 arg1Of site,the
R8823 T11799 T11798 arg1Of site,GATA3
R8824 T11801 T11793 arg1Of we,address
R8825 T11801 T11802 arg1Of we,inserted
R8826 T11802 T11791 modOf inserted,To
R8827 T11802 T11800 arg1Of inserted,","
R8828 T11805 T11802 arg2Of mutation,inserted
R8829 T11805 T11803 arg1Of mutation,a
R8830 T11805 T11804 arg1Of mutation,site-specific
R8831 T11805 T11806 arg1Of mutation,deleting
R8832 T11809 T11806 arg2Of site,deleting
R8833 T11809 T11807 arg1Of site,the
R8834 T11809 T11808 arg1Of site,GATA3-binding
R8835 T11811 T11810 arg1Of mutation,This
R8836 T11811 T11812 arg1Of mutation,increased
R8837 T11812 T11815 arg1Of increased,by
R8838 T11812 T11817 arg1Of increased,in
R8839 T11812 T11822 arg1Of increased,","
R8840 T11812 T11823 arg1Of increased,whereas
R8841 T11814 T11812 arg2Of activity,increased
R8842 T11814 T11813 arg1Of activity,luciferase
R8843 T11816 T11815 arg2Of 3-fold,by
R8844 T11821 T11817 arg2Of cells,in
R8845 T11821 T11818 arg1Of cells,memory
R8846 T11821 T11819 arg1Of cells,CD4+CD45RO+
R8847 T11821 T11820 arg1Of cells,T
R8848 T11825 T11824 arg1Of difference,no
R8849 T11825 T11826 arg1Of difference,was
R8850 T11825 T11827 arg2Of difference,observed
R8851 T11827 T11823 arg2Of observed,whereas
R8852 T11827 T11826 arg2Of observed,was
R8853 T11827 T11828 arg1Of observed,in
R8854 T11827 T11836 arg1Of observed,","
R8855 T11827 T11837 modOf observed,revealing
R8856 T11831 T11830 arg2Of GATA3–,(
R8857 T11832 T11830 arg3Of ),(
R8858 T11835 T11828 arg2Of cells,in
R8859 T11835 T11829 arg1Of cells,naive
R8860 T11835 T11830 arg1Of cells,(
R8861 T11835 T11833 arg1Of cells,CD4+CD45RA+
R8862 T11835 T11834 arg1Of cells,T
R8863 T11840 T11837 arg2Of activity,revealing
R8864 T11840 T11838 arg1Of activity,a
R8865 T11840 T11839 arg1Of activity,repressor
R8866 T11840 T11841 arg1Of activity,of
R8867 T11840 T11843 arg1Of activity,on
R8868 T11842 T11841 arg2Of GATA3,of
R8869 T11846 T11843 arg2Of promoter,on
R8870 T11846 T11844 arg1Of promoter,the
R8871 T11846 T11845 arg1Of promoter,FOXP3
R8872 T11846 T11847 arg1Of promoter,(
R8873 T11849 T11847 arg2Of 8C,(
R8874 T11849 T11848 arg1Of 8C,Figure
R8875 T11850 T11847 arg3Of ),(
R8876 T11852 T11853 arg1Of GATA3,binds
R8877 T11853 T11851 arg1Of binds,Furthermore
R8878 T11853 T11854 arg1Of binds,directly
R8879 T11853 T11855 arg1Of binds,to
R8880 T11853 T11859 arg1Of binds,as
R8881 T11858 T11855 arg2Of promoter,to
R8882 T11858 T11856 arg1Of promoter,the
R8883 T11858 T11857 arg1Of promoter,FOXP3
R8884 T11860 T11859 arg2Of investigated,as
R8885 T11863 T11860 arg1Of assay,investigated
R8886 T11863 T11861 arg2Of assay,by
R8887 T11863 T11862 arg1Of assay,pull-down
R8888 T11865 T11864 arg1Of cells,HEK
R8889 T11865 T11866 arg1Of cells,were
R8890 T11865 T11868 arg2Of cells,transfected
R8891 T11868 T11866 arg2Of transfected,were
R8892 T11868 T11867 arg1Of transfected,transiently
R8893 T11868 T11869 arg1Of transfected,with
R8894 T11868 T11876 arg1Of transfected,and
R8895 T11870 T11871 arg1Of GATA3,or
R8896 T11871 T11869 arg2Of or,with
R8897 T11874 T11871 arg2Of vector,or
R8898 T11874 T11872 arg1Of vector,a
R8899 T11874 T11873 arg1Of vector,control
R8900 T11876 T11875 arg1Of and,","
R8901 T11878 T11877 arg1Of amounts,increasing
R8902 T11878 T11879 arg1Of amounts,of
R8903 T11878 T11881 arg1Of amounts,were
R8904 T11878 T11882 arg2Of amounts,incubated
R8905 T11880 T11879 arg2Of lysates,of
R8906 T11882 T11876 arg2Of incubated,and
R8907 T11882 T11881 arg2Of incubated,were
R8908 T11882 T11883 arg1Of incubated,with
R8909 T11884 T11883 arg2Of oligonucleotides,with
R8910 T11884 T11885 arg1Of oligonucleotides,containing
R8911 T11888 T11886 arg1Of site,the
R8912 T11888 T11887 arg1Of site,GATA3
R8913 T11888 T11889 arg1Of site,of
R8914 T11888 T11893 arg1Of site,or
R8915 T11892 T11889 arg2Of promoter,of
R8916 T11892 T11890 arg1Of promoter,the
R8917 T11892 T11891 arg1Of promoter,FOXP3
R8918 T11893 T11885 arg2Of or,containing
R8919 T11896 T11893 arg2Of oligonucleotide,or
R8920 T11896 T11894 arg1Of oligonucleotide,a
R8921 T11896 T11895 arg1Of oligonucleotide,control
R8922 T11896 T11897 arg1Of oligonucleotide,with
R8923 T11901 T11897 arg2Of site,with
R8924 T11901 T11898 arg1Of site,a
R8925 T11901 T11899 arg2Of site,mutated
R8926 T11901 T11900 arg1Of site,GATA3-binding
R8927 T11904 T11902 arg2Of pull-down,After
R8928 T11904 T11903 arg1Of pull-down,the
R8929 T11907 T11906 arg1Of binding,GATA3
R8930 T11907 T11908 arg1Of binding,was
R8931 T11907 T11909 arg2Of binding,detected
R8932 T11909 T11902 arg1Of detected,After
R8933 T11909 T11905 arg1Of detected,","
R8934 T11909 T11908 arg2Of detected,was
R8935 T11912 T11909 arg1Of blot,detected
R8936 T11912 T11910 arg2Of blot,by
R8937 T11912 T11911 arg1Of blot,Western
R8938 T11917 T11915 arg1Of cells,GATA3-expressing
R8939 T11917 T11916 arg1Of cells,Th2
R8940 T11917 T11918 arg1Of cells,and
R8941 T11918 T11921 arg1Of and,were
R8942 T11918 T11922 arg2Of and,subjected
R8943 T11920 T11918 arg2Of cells,and
R8944 T11920 T11919 arg1Of cells,iTreg
R8945 T11922 T11913 arg1Of subjected,Similarly
R8946 T11922 T11914 arg1Of subjected,","
R8947 T11922 T11921 arg2Of subjected,were
R8948 T11922 T11923 arg1Of subjected,to
R8949 T11925 T11923 arg2Of approach,to
R8950 T11925 T11924 arg1Of approach,this
R8951 T11928 T11926 arg1Of cells,Only
R8952 T11928 T11927 arg1Of cells,HEK
R8953 T11928 T11929 arg1Of cells,overexpressing
R8954 T11928 T11934 arg1Of cells,showed
R8955 T11930 T11931 arg1Of GATA3,and
R8956 T11932 T11931 arg2Of Th2,and
R8957 T11933 T11929 arg2Of cells,overexpressing
R8958 T11933 T11930 arg1Of cells,GATA3
R8959 T11933 T11932 arg1Of cells,Th2
R8960 T11936 T11934 arg2Of activity,showed
R8961 T11936 T11935 arg1Of activity,GATA3-binding
R8962 T11936 T11937 arg1Of activity,(
R8963 T11939 T11938 arg1Of 8D,Figure
R8964 T11939 T11940 arg1Of 8D,and
R8965 T11940 T11937 arg2Of and,(
R8966 T11941 T11940 arg2Of 8E,and
R8967 T11942 T11937 arg3Of ),(
R8968 T11944 T11943 arg1Of experiments,These
R8969 T11944 T11945 arg1Of experiments,demonstrated
R8970 T11947 T11948 arg1Of GATA3,binds
R8971 T11948 T11945 arg2Of binds,demonstrated
R8972 T11948 T11946 arg1Of binds,that
R8973 T11952 T11948 arg2Of promoter,binds
R8974 T11952 T11949 arg1Of promoter,the
R8975 T11952 T11950 arg1Of promoter,palindromic
R8976 T11952 T11951 arg1Of promoter,FOXP3
R8977 T11954 T11953 arg1Of gain,To
R8978 T11955 T11954 arg2Of insights,gain
R8979 T11955 T11956 arg1Of insights,into
R8980 T11959 T11958 arg1Of vivo,in
R8981 T11960 T11956 arg2Of situation,into
R8982 T11960 T11957 arg1Of situation,the
R8983 T11960 T11959 arg1Of situation,vivo
R8984 T11962 T11954 arg1Of we,gain
R8985 T11962 T11963 arg1Of we,performed
R8986 T11962 T11970 arg1Of we,using
R8987 T11962 T11975 arg1Of we,showed
R8988 T11963 T11970 modOf performed,using
R8989 T11963 T11974 arg1Of performed,and
R8990 T11966 T11963 arg2Of immunoprecipitation,performed
R8991 T11966 T11964 arg1Of immunoprecipitation,a
R8992 T11966 T11965 arg1Of immunoprecipitation,chromatin
R8993 T11966 T11967 arg1Of immunoprecipitation,(
R8994 T11968 T11967 arg2Of ChIP,(
R8995 T11969 T11967 arg3Of ),(
R8996 T11973 T11970 arg2Of antibody,using
R8997 T11973 T11971 arg1Of antibody,an
R8998 T11973 T11972 arg1Of antibody,anti-GATA3
R8999 T11974 T11953 modOf and,To
R9000 T11974 T11961 arg1Of and,","
R9001 T11975 T11974 arg2Of showed,and
R9002 T11977 T11978 arg1Of GATA3,binds
R9003 T11978 T11975 arg2Of binds,showed
R9004 T11978 T11976 arg1Of binds,that
R9005 T11978 T11979 arg1Of binds,to
R9006 T11978 T11990 arg1Of binds,in
R9007 T11979 T11988 arg1Of to,but
R9008 T11983 T11979 arg2Of region,to
R9009 T11983 T11980 arg1Of region,the
R9010 T11983 T11981 arg1Of region,FOXP3
R9011 T11983 T11982 arg1Of region,promoter
R9012 T11983 T11984 arg1Of region,in
R9013 T11986 T11984 arg2Of cells,in
R9014 T11986 T11985 arg1Of cells,Th2
R9015 T11988 T11987 arg1Of but,","
R9016 T11990 T11988 arg2Of in,but
R9017 T11990 T11989 arg1Of in,not
R9018 T11992 T11990 arg2Of cells,in
R9019 T11992 T11991 arg1Of cells,iTreg
R9020 T11992 T11993 arg1Of cells,(
R9021 T11995 T11993 arg2Of 7F,(
R9022 T11995 T11994 arg1Of 7F,Figure
R9023 T11996 T11993 arg3Of ),(
R9024 T11997 T11998 arg1Of Taken,together
R9025 T12000 T11999 arg1Of data,these
R9026 T12000 T12001 arg1Of data,demonstrate
R9027 T12001 T11997 modOf demonstrate,Taken
R9028 T12004 T12003 arg1Of GATA3-binding,the
R9029 T12004 T12005 arg1Of GATA3-binding,to
R9030 T12004 T12009 arg1Of GATA3-binding,is
R9031 T12004 T12010 arg1Of GATA3-binding,repressing
R9032 T12008 T12005 arg2Of promoter,to
R9033 T12008 T12006 arg1Of promoter,the
R9034 T12008 T12007 arg1Of promoter,FOXP3
R9035 T12010 T12001 arg2Of repressing,demonstrate
R9036 T12010 T12002 arg1Of repressing,that
R9037 T12010 T12009 arg2Of repressing,is
R9038 T12012 T12010 arg2Of expression,repressing
R9039 T12012 T12011 arg1Of expression,FOXP3
R10080 T13706 T13704 arg1Of study,The
R10081 T13706 T13705 arg1Of study,current
R10082 T13706 T13707 arg1Of study,reveals
R10083 T13710 T13709 arg1Of induction,FOXP3
R10084 T13710 T13711 arg1Of induction,","
R10085 T13710 T13719 arg1Of induction,is
R10086 T13710 T13720 arg2Of induction,inhibited
R10087 T13714 T13711 arg2Of step,","
R10088 T13714 T13712 arg1Of step,an
R10089 T13714 T13713 arg1Of step,important
R10090 T13714 T13715 arg1Of step,in
R10091 T13717 T13715 arg2Of commitment,in
R10092 T13717 T13716 arg1Of commitment,iTreg
R10093 T13720 T13707 arg2Of inhibited,reveals
R10094 T13720 T13708 arg1Of inhibited,that
R10095 T13720 T13718 arg1Of inhibited,","
R10096 T13720 T13719 arg2Of inhibited,is
R10097 T13722 T13720 arg1Of GATA3,inhibited
R10098 T13722 T13721 arg2Of GATA3,by
R10099 T13722 T13723 arg1Of GATA3,","
R10100 T13722 T13724 arg1Of GATA3,which
R10101 T13722 T13725 arg1Of GATA3,is
R10102 T13728 T13725 arg2Of regulator,is
R10103 T13728 T13726 arg1Of regulator,the
R10104 T13728 T13727 arg1Of regulator,key
R10105 T13728 T13729 arg1Of regulator,for
R10106 T13730 T13729 arg2Of polarization,for
R10107 T13730 T13731 arg1Of polarization,toward
R10108 T13733 T13731 arg2Of cells,toward
R10109 T13733 T13732 arg1Of cells,Th2
R10110 T13735 T13734 arg2Of differentiation,After
R10111 T13740 T13737 arg1Of cells,the
R10112 T13740 T13738 arg1Of cells,effector
R10113 T13740 T13739 arg1Of cells,Th2
R10114 T13740 T13741 arg1Of cells,become
R10115 T13740 T13742 arg1Of cells,refractory
R10116 T13741 T13734 arg1Of become,After
R10117 T13741 T13736 arg1Of become,","
R10118 T13742 T13741 arg2Of refractory,become
R10119 T13742 T13743 arg1Of refractory,to
R10120 T13744 T13743 arg2Of conversion,to
R10121 T13744 T13745 arg1Of conversion,into
R10122 T13748 T13745 arg2Of phenotype,into
R10123 T13748 T13746 arg1Of phenotype,a
R10124 T13748 T13747 arg1Of phenotype,FOXP3+
R10125 T13750 T13749 arg2Of accordance,In
R10126 T13750 T13751 arg1Of accordance,with
R10127 T13753 T13751 arg2Of studies,with
R10128 T13753 T13752 arg1Of studies,other
R10129 T13755 T13756 arg1Of we,found
R10130 T13756 T13749 arg1Of found,In
R10131 T13756 T13754 arg1Of found,","
R10132 T13759 T13758 arg1Of cells,CD4+CD25–
R10133 T13759 T13760 arg1Of cells,were
R10134 T13759 T13761 arg1Of cells,able
R10135 T13759 T13763 arg1Of cells,up-regulate
R10136 T13760 T13756 arg2Of were,found
R10137 T13760 T13757 arg1Of were,that
R10138 T13761 T13760 arg2Of able,were
R10139 T13763 T13761 arg2Of up-regulate,able
R10140 T13763 T13762 arg1Of up-regulate,to
R10141 T13763 T13765 arg1Of up-regulate,[
R10142 T13764 T13763 arg2Of FOXP3,up-regulate
R10143 T13766 T13765 arg2Of "12,27",[
R10144 T13767 T13765 arg3Of ],[
R10145 T13769 T13768 arg1Of cells,Already-committed
R10146 T13769 T13771 arg1Of cells,as
R10147 T13769 T13778 arg1Of cells,showed
R10148 T13771 T13770 arg1Of as,such
R10149 T13774 T13772 arg1Of cells,memory
R10150 T13774 T13773 arg1Of cells,T
R10151 T13774 T13775 arg1Of cells,and
R10152 T13775 T13771 arg2Of and,as
R10153 T13777 T13775 arg2Of cells,and
R10154 T13777 T13776 arg1Of cells,Th1
R10155 T13780 T13781 arg1Of moderate,and
R10156 T13781 T13779 arg1Of and,only
R10157 T13782 T13781 arg2Of transient,and
R10158 T13784 T13778 arg2Of induction,showed
R10159 T13784 T13780 arg1Of induction,moderate
R10160 T13784 T13782 arg1Of induction,transient
R10161 T13784 T13783 arg1Of induction,FOXP3
R10162 T13784 T13785 arg1Of induction,","
R10163 T13784 T13786 arg1Of induction,which
R10164 T13784 T13787 arg1Of induction,is
R10165 T13784 T13789 arg1Of induction,sufficient
R10166 T13784 T13791 arg1Of induction,change
R10167 T13787 T13788 arg1Of is,not
R10168 T13789 T13787 arg2Of sufficient,is
R10169 T13791 T13789 arg2Of change,sufficient
R10170 T13791 T13790 arg1Of change,to
R10171 T13791 T13794 arg1Of change,toward
R10172 T13793 T13791 arg2Of phenotype,change
R10173 T13793 T13792 arg1Of phenotype,the
R10174 T13799 T13794 arg2Of profile,toward
R10175 T13799 T13795 arg1Of profile,a
R10176 T13799 T13796 arg1Of profile,regulatory
R10177 T13799 T13797 arg1Of profile,T
R10178 T13799 T13798 arg1Of profile,cell
R10179 T13801 T13800 arg2Of contrast,In
R10180 T13805 T13803 arg1Of cells,naive
R10181 T13805 T13804 arg1Of cells,T
R10182 T13805 T13806 arg1Of cells,could
R10183 T13805 T13808 arg1Of cells,up-regulate
R10184 T13808 T13800 arg1Of up-regulate,In
R10185 T13808 T13802 arg1Of up-regulate,","
R10186 T13808 T13806 arg2Of up-regulate,could
R10187 T13808 T13807 arg1Of up-regulate,efficiently
R10188 T13808 T13810 arg1Of up-regulate,when
R10189 T13808 T13821 arg1Of up-regulate,","
R10190 T13808 T13822 modOf up-regulate,suggesting
R10191 T13809 T13808 arg2Of FOXP3,up-regulate
R10192 T13811 T13810 arg2Of treated,when
R10193 T13811 T13812 arg1Of treated,with
R10194 T13811 T13814 modOf treated,to
R10195 T13813 T13812 arg2Of TGF-β,with
R10196 T13815 T13814 arg1Of induce,to
R10197 T13817 T13815 arg2Of cells,induce
R10198 T13817 T13816 arg1Of cells,iTreg
R10199 T13817 T13818 arg1Of cells,[
R10200 T13819 T13818 arg2Of "10,28–34",[
R10201 T13820 T13818 arg3Of ],[
R10202 T13824 T13825 arg1Of FOXP3,plays
R10203 T13824 T13835 arg1Of FOXP3,may
R10204 T13824 T13836 arg1Of FOXP3,act
R10205 T13825 T13829 arg1Of plays,in
R10206 T13825 T13834 arg1Of plays,and
R10207 T13828 T13825 arg2Of role,plays
R10208 T13828 T13826 arg1Of role,an
R10209 T13828 T13827 arg1Of role,important
R10210 T13833 T13829 arg2Of process,in
R10211 T13833 T13830 arg1Of process,the
R10212 T13833 T13831 arg1Of process,early
R10213 T13833 T13832 arg1Of process,differentiation
R10214 T13834 T13822 arg2Of and,suggesting
R10215 T13834 T13823 arg1Of and,that
R10216 T13836 T13834 arg2Of act,and
R10217 T13836 T13835 arg2Of act,may
R10218 T13836 T13837 arg1Of act,in
R10219 T13839 T13837 arg2Of way,in
R10220 T13839 T13838 arg1Of way,a
R10221 T13839 T13840 arg1Of way,similar
R10222 T13840 T13841 arg1Of similar,to
R10223 T13842 T13841 arg2Of that,to
R10224 T13842 T13843 arg2Of that,known
R10225 T13843 T13844 arg1Of known,for
R10226 T13849 T13850 arg1Of T-bet,and
R10227 T13850 T13844 arg2Of and,for
R10228 T13850 T13845 arg1Of and,the
R10229 T13850 T13846 arg1Of and,Th1/Th2
R10230 T13850 T13847 arg1Of and,decision
R10231 T13850 T13848 arg1Of and,factors
R10232 T13851 T13850 arg2Of GATA3,and
R10233 T13853 T13852 arg1Of commitment,This
R10234 T13853 T13854 arg1Of commitment,is
R10235 T13853 T13855 arg2Of commitment,characterized
R10236 T13855 T13854 arg2Of characterized,is
R10237 T13855 T13865 arg1Of characterized,","
R10238 T13858 T13855 arg1Of expression,characterized
R10239 T13858 T13856 arg2Of expression,by
R10240 T13858 T13857 arg1Of expression,competitive
R10241 T13858 T13859 arg1Of expression,of
R10242 T13858 T13862 arg1Of expression,[
R10243 T13861 T13859 arg2Of factors,of
R10244 T13861 T13860 arg1Of factors,these
R10245 T13863 T13862 arg2Of "35,36",[
R10246 T13864 T13862 arg3Of ],[
R10247 T13866 T13869 arg2Of which,observed
R10248 T13867 T13869 arg1Of we,observed
R10249 T13869 T13854 modOf observed,is
R10250 T13869 T13868 arg1Of observed,also
R10251 T13869 T13870 arg1Of observed,in
R10252 T13874 T13870 arg2Of cells,in
R10253 T13874 T13871 arg1Of cells,differentiating
R10254 T13874 T13872 arg1Of cells,FOXP3+
R10255 T13874 T13873 arg1Of cells,iTreg
R10256 T13874 T13875 arg1Of cells,including
R10257 T13877 T13875 arg2Of phase,including
R10258 T13877 T13876 arg1Of phase,a
R10259 T13877 T13878 arg1Of phase,of
R10260 T13877 T13880 arg1Of phase,","
R10261 T13877 T13881 arg1Of phase,which
R10262 T13877 T13882 arg1Of phase,turns
R10263 T13879 T13878 arg2Of co-expression,of
R10264 T13882 T13883 arg1Of turns,into
R10265 T13882 T13886 arg1Of turns,upon
R10266 T13885 T13883 arg2Of expression,into
R10267 T13885 T13884 arg1Of expression,nonoverlapping
R10268 T13888 T13886 arg2Of differentiation,upon
R10269 T13888 T13887 arg2Of differentiation,completed
R10270 T13892 T13889 arg2Of process,In
R10271 T13892 T13890 arg1Of process,this
R10272 T13892 T13891 arg1Of process,competitive
R10273 T13893 T13894 arg1Of TGF-β,appeared
R10274 T13893 T13896 arg1Of TGF-β,be
R10275 T13893 T13897 arg1Of TGF-β,mandatory
R10276 T13893 T13906 arg1Of TGF-β,keeping
R10277 T13894 T13889 arg1Of appeared,In
R10278 T13896 T13894 arg2Of be,appeared
R10279 T13896 T13895 arg1Of be,to
R10280 T13896 T13903 arg1Of be,","
R10281 T13896 T13905 arg1Of be,by
R10282 T13897 T13896 arg2Of mandatory,be
R10283 T13897 T13898 arg1Of mandatory,for
R10284 T13900 T13898 arg2Of induction,for
R10285 T13900 T13899 arg1Of induction,the
R10286 T13900 T13901 arg1Of induction,of
R10287 T13902 T13901 arg2Of FOXP3,of
R10288 T13905 T13904 arg1Of by,possibly
R10289 T13906 T13905 arg2Of keeping,by
R10290 T13908 T13906 arg2Of expression,keeping
R10291 T13908 T13907 arg1Of expression,the
R10292 T13908 T13909 arg1Of expression,of
R10293 T13910 T13911 arg1Of GATA3,and
R10294 T13912 T13911 arg2Of T-bet,and
R10295 T13913 T13909 arg2Of low,of
R10296 T13913 T13910 arg1Of low,GATA3
R10297 T13913 T13912 arg1Of low,T-bet
R10298 T13913 T13914 arg1Of low,[
R10299 T13915 T13914 arg2Of "37,38",[
R10300 T13916 T13914 arg3Of ],[
R10301 T13918 T13917 arg2Of contrast,In
R10302 T13923 T13920 arg1Of cells,differentiating
R10303 T13923 T13921 arg1Of cells,naive
R10304 T13923 T13922 arg1Of cells,T
R10305 T13923 T13924 arg1Of cells,in
R10306 T13923 T13941 arg1Of cells,showed
R10307 T13923 T13948 arg1Of cells,failed
R10308 T13923 T13950 arg1Of cells,generate
R10309 T13926 T13924 arg2Of absence,in
R10310 T13926 T13925 arg1Of absence,the
R10311 T13926 T13927 arg1Of absence,of
R10312 T13929 T13927 arg2Of factors,of
R10313 T13929 T13928 arg1Of factors,polarization
R10314 T13929 T13930 arg1Of factors,(
R10315 T13929 T13934 arg1Of factors,as
R10316 T13931 T13930 arg2Of Th0,(
R10317 T13932 T13930 arg3Of ),(
R10318 T13934 T13933 arg1Of as,such
R10319 T13935 T13936 arg1Of IL-4,","
R10320 T13936 T13939 arg1Of ",",or
R10321 T13937 T13936 arg2Of IL-12,","
R10322 T13939 T13934 arg2Of or,as
R10323 T13939 T13938 arg1Of or,","
R10324 T13940 T13939 arg2Of TGF-β,or
R10325 T13941 T13947 arg1Of showed,and
R10326 T13946 T13941 arg2Of expression,showed
R10327 T13946 T13942 arg1Of expression,only
R10328 T13946 T13943 arg1Of expression,a
R10329 T13946 T13944 arg1Of expression,transient
R10330 T13946 T13945 arg1Of expression,FOXP3
R10331 T13947 T13957 arg1Of and,but
R10332 T13948 T13947 arg2Of failed,and
R10333 T13950 T13948 arg2Of generate,failed
R10334 T13950 T13949 arg1Of generate,to
R10335 T13952 T13950 arg2Of population,generate
R10336 T13952 T13951 arg1Of population,a
R10337 T13952 T13953 arg1Of population,of
R10338 T13955 T13953 arg2Of cells,of
R10339 T13955 T13954 arg1Of cells,FOXP3-expressing
R10340 T13957 T13917 arg1Of but,In
R10341 T13957 T13919 arg1Of but,","
R10342 T13957 T13956 arg1Of but,","
R10343 T13958 T13959 arg1Of GATA3,and
R10344 T13959 T13961 arg1Of and,were
R10345 T13959 T13962 arg2Of and,up-regulated
R10346 T13960 T13959 arg2Of T-bet,and
R10347 T13962 T13957 arg2Of up-regulated,but
R10348 T13962 T13961 arg2Of up-regulated,were
R10349 T13962 T13963 arg1Of up-regulated,(
R10350 T13965 T13963 arg2Of data,(
R10351 T13965 T13964 arg1Of data,unpublished
R10352 T13966 T13963 arg3Of ),(
R10353 T13967 T13968 arg1Of Interestingly,","
R10354 T13969 T13968 arg2Of as,","
R10355 T13972 T13969 arg2Of others,as
R10356 T13972 T13970 arg1Of others,we
R10357 T13972 T13971 arg1Of others,and
R10358 T13972 T13973 arg1Of others,previously
R10359 T13974 T13975 arg1Of described,","
R10360 T13976 T13975 arg2Of FOXP3-promoting,","
R10361 T13977 T13974 arg1Of factors,described
R10362 T13977 T13976 arg1Of factors,FOXP3-promoting
R10363 T13977 T13978 arg1Of factors,","
R10364 T13977 T13980 arg1Of factors,as
R10365 T13977 T13991 arg1Of factors,and
R10366 T13980 T13979 arg1Of as,such
R10367 T13981 T13982 arg1Of dexamethasone,[
R10368 T13981 T13985 arg1Of dexamethasone,","
R10369 T13983 T13982 arg2Of 39,[
R10370 T13984 T13982 arg3Of ],[
R10371 T13985 T13980 arg2Of ",",as
R10372 T13986 T13985 arg2Of CTLA-4,","
R10373 T13986 T13987 arg1Of CTLA-4,[
R10374 T13988 T13987 arg2Of 40,[
R10375 T13989 T13987 arg3Of ],[
R10376 T13991 T13990 arg1Of and,","
R10377 T13991 T13997 arg1Of and,are
R10378 T13991 T13999 arg2Of and,known
R10379 T13992 T13991 arg2Of estrogens,and
R10380 T13992 T13993 arg1Of estrogens,[
R10381 T13994 T13993 arg2Of 41,[
R10382 T13995 T13993 arg3Of ],[
R10383 T13999 T13967 arg1Of known,Interestingly
R10384 T13999 T13969 arg1Of known,as
R10385 T13999 T13996 arg1Of known,","
R10386 T13999 T13997 arg2Of known,are
R10387 T13999 T13998 arg1Of known,also
R10388 T13999 T14000 arg1Of known,as
R10389 T14001 T14000 arg2Of inhibitors,as
R10390 T14001 T14002 arg1Of inhibitors,of
R10391 T14004 T14002 arg2Of expression,of
R10392 T14004 T14003 arg1Of expression,GATA3
R10393 T14004 T14005 arg1Of expression,[
R10394 T14006 T14005 arg2Of 42–45,[
R10395 T14007 T14005 arg3Of ],[
R10396 T14009 T14012 arg1Of GATA3,induces
R10397 T14009 T14019 arg1Of GATA3,inhibits
R10398 T14011 T14010 arg1Of only,not
R10399 T14012 T14014 arg1Of induces,into
R10400 T14012 T14017 arg1Of induces,but
R10401 T14013 T14012 arg2Of differentiation,induces
R10402 T14016 T14014 arg2Of cells,into
R10403 T14016 T14015 arg1Of cells,Th2
R10404 T14017 T14008 arg1Of but,Therefore
R10405 T14017 T14011 arg1Of but,only
R10406 T14017 T14018 arg1Of but,also
R10407 T14019 T14017 arg2Of inhibits,but
R10408 T14021 T14022 arg1Of expression,and
R10409 T14022 T14019 arg2Of and,inhibits
R10410 T14022 T14020 arg1Of and,FOXP3
R10411 T14022 T14024 arg1Of and,into
R10412 T14023 T14022 arg2Of commitment,and
R10413 T14026 T14024 arg2Of cells,into
R10414 T14026 T14025 arg1Of cells,iTreg
R10415 T14029 T14027 arg1Of cytokine,The
R10416 T14029 T14028 arg1Of cytokine,Th2
R10417 T14030 T14029 arg1Of IL-4,cytokine
R10418 T14030 T14031 arg1Of IL-4,but
R10419 T14031 T14038 arg1Of but,was
R10420 T14031 T14039 arg1Of but,able
R10421 T14031 T14041 arg1Of but,inhibit
R10422 T14031 T14047 arg1Of but,prevented
R10423 T14033 T14031 arg2Of IL-13,but
R10424 T14033 T14032 arg1Of IL-13,not
R10425 T14033 T14034 arg1Of IL-13,(
R10426 T14036 T14034 arg2Of data,(
R10427 T14036 T14035 arg1Of data,unpublished
R10428 T14037 T14034 arg3Of ),(
R10429 T14038 T14045 arg1Of was,and
R10430 T14039 T14038 arg2Of able,was
R10431 T14041 T14039 arg2Of inhibit,able
R10432 T14041 T14040 arg1Of inhibit,to
R10433 T14044 T14041 arg2Of induction,inhibit
R10434 T14044 T14042 arg1Of induction,TGF-β–mediated
R10435 T14044 T14043 arg1Of induction,FOXP3
R10436 T14047 T14045 arg2Of prevented,and
R10437 T14047 T14046 arg1Of prevented,therefore
R10438 T14048 T14047 arg2Of conversion,prevented
R10439 T14048 T14049 arg1Of conversion,into
R10440 T14052 T14049 arg2Of phenotype,into
R10441 T14052 T14050 arg1Of phenotype,the
R10442 T14052 T14051 arg1Of phenotype,regulatory
R10443 T14054 T14053 arg1Of prove,To
R10444 T14054 T14067 arg1Of prove,vivo
R10445 T14057 T14054 arg2Of effect,prove
R10446 T14057 T14055 arg1Of effect,the
R10447 T14057 T14056 arg1Of effect,inhibitory
R10448 T14057 T14058 arg1Of effect,of
R10449 T14057 T14060 arg1Of effect,on
R10450 T14059 T14058 arg2Of IL-4,of
R10451 T14061 T14062 arg1Of inducible,or
R10452 T14063 T14062 arg2Of natural,or
R10453 T14065 T14060 arg2Of commitment,on
R10454 T14065 T14061 arg1Of commitment,inducible
R10455 T14065 T14063 arg1Of commitment,natural
R10456 T14065 T14064 arg1Of commitment,Treg
R10457 T14067 T14066 arg1Of vivo,in
R10458 T14069 T14054 arg1Of we,prove
R10459 T14069 T14070 arg1Of we,treated
R10460 T14070 T14053 modOf treated,To
R10461 T14070 T14068 arg1Of treated,","
R10462 T14071 T14070 arg2Of mice,treated
R10463 T14071 T14072 arg1Of mice,with
R10464 T14073 T14074 arg1Of IL-4,and
R10465 T14074 T14072 arg2Of and,with
R10466 T14075 T14074 arg2Of anti-IL-4,and
R10467 T14076 T14077 arg1Of This,has
R10468 T14076 T14078 arg1Of This,been
R10469 T14076 T14079 arg2Of This,shown
R10470 T14076 T14081 arg1Of This,increase
R10471 T14079 T14077 arg2Of shown,has
R10472 T14079 T14078 arg2Of shown,been
R10473 T14081 T14079 arg3Of increase,shown
R10474 T14081 T14080 arg1Of increase,to
R10475 T14081 T14089 arg1Of increase,[
R10476 T14083 T14081 arg2Of effect,increase
R10477 T14083 T14082 arg1Of effect,the
R10478 T14083 T14084 arg1Of effect,of
R10479 T14083 T14088 arg1Of effect,vivo
R10480 T14086 T14084 arg2Of cytokine,of
R10481 T14086 T14085 arg1Of cytokine,the
R10482 T14088 T14087 arg1Of vivo,in
R10483 T14090 T14089 arg2Of 19,[
R10484 T14091 T14089 arg3Of ],[
R10485 T14096 T14092 arg1Of complex,Only
R10486 T14096 T14093 arg1Of complex,the
R10487 T14096 T14094 arg1Of complex,IL-4/IL-4
R10488 T14096 T14095 arg1Of complex,mAb
R10489 T14096 T14097 arg1Of complex,resulted
R10490 T14097 T14098 arg1Of resulted,in
R10491 T14100 T14098 arg2Of decrease,in
R10492 T14100 T14099 arg1Of decrease,a
R10493 T14100 T14101 arg1Of decrease,of
R10494 T14103 T14102 arg1Of amount,the
R10495 T14103 T14104 arg1Of amount,of
R10496 T14103 T14111 arg1Of amount,and
R10497 T14105 T14106 arg1Of natural,or
R10498 T14107 T14106 arg2Of inducible,or
R10499 T14108 T14105 arg1Of Treg,natural
R10500 T14108 T14107 arg1Of Treg,inducible
R10501 T14108 T14109 arg1Of Treg,(
R10502 T14110 T14104 arg2Of CD25+,of
R10503 T14110 T14108 arg1Of CD25+,Treg
R10504 T14111 T14101 arg2Of and,of
R10505 T14116 T14111 arg2Of d,and
R10506 T14116 T14112 arg1Of d,Foxp3+
R10507 T14116 T14113 arg1Of d,)
R10508 T14116 T14114 arg1Of d,cells
R10509 T14116 T14115 arg1Of d,7
R10510 T14116 T14117 arg1Of d,after
R10511 T14118 T14117 arg2Of treatment,after
R10512 T14120 T14119 arg1Of results,Our
R10513 T14120 T14121 arg1Of results,suggest
R10514 T14123 T14124 arg1Of IL-4,is
R10515 T14123 T14126 arg1Of IL-4,interfering
R10516 T14126 T14121 arg2Of interfering,suggest
R10517 T14126 T14122 arg1Of interfering,that
R10518 T14126 T14124 arg2Of interfering,is
R10519 T14126 T14125 arg1Of interfering,only
R10520 T14126 T14127 arg1Of interfering,with
R10521 T14129 T14127 arg2Of differentiation,with
R10522 T14129 T14128 arg1Of differentiation,the
R10523 T14129 T14130 arg1Of differentiation,of
R10524 T14129 T14134 arg1Of differentiation,into
R10525 T14133 T14130 arg2Of cells,of
R10526 T14133 T14131 arg1Of cells,naive
R10527 T14133 T14132 arg1Of cells,T
R10528 T14136 T14134 arg2Of cells,into
R10529 T14136 T14135 arg1Of cells,iTreg
R10530 T14140 T14139 arg1Of distinction,a
R10531 T14140 T14141 arg1Of distinction,of
R10532 T14140 T14146 arg1Of distinction,is
R10533 T14140 T14149 arg1Of distinction,possible
R10534 T14142 T14143 arg1Of nTreg,and
R10535 T14144 T14143 arg2Of iTreg,and
R10536 T14145 T14141 arg2Of cells,of
R10537 T14145 T14142 arg1Of cells,nTreg
R10538 T14145 T14144 arg1Of cells,iTreg
R10539 T14146 T14138 arg2Of is,since
R10540 T14146 T14147 arg1Of is,currently
R10541 T14146 T14148 arg1Of is,not
R10542 T14146 T14150 arg1Of is,","
R10543 T14146 T14151 arg1Of is,because
R10544 T14149 T14146 arg2Of possible,is
R10545 T14153 T14152 arg1Of cells,iTreg
R10546 T14153 T14156 arg1Of cells,express
R10547 T14156 T14151 arg2Of express,because
R10548 T14156 T14154 arg1Of express,also
R10549 T14156 T14155 arg1Of express,transiently
R10550 T14156 T14158 arg1Of express,after
R10551 T14157 T14156 arg2Of CD25,express
R10552 T14159 T14158 arg2Of activation,after
R10553 T14159 T14164 arg2Of activation,exclude
R10554 T14161 T14162 arg1Of we,can
R10555 T14161 T14164 arg1Of we,exclude
R10556 T14164 T14160 arg1Of exclude,","
R10557 T14164 T14162 arg2Of exclude,can
R10558 T14164 T14163 arg1Of exclude,not
R10559 T14166 T14167 arg1Of IL-4,may
R10560 T14166 T14169 arg1Of IL-4,inhibit
R10561 T14169 T14165 arg1Of inhibit,that
R10562 T14169 T14167 arg2Of inhibit,may
R10563 T14169 T14168 arg1Of inhibit,also
R10564 T14169 T14176 arg1Of inhibit,vivo
R10565 T14169 T14177 arg1Of inhibit,or
R10566 T14171 T14169 arg2Of expression,inhibit
R10567 T14171 T14170 arg1Of expression,Foxp3
R10568 T14171 T14172 arg1Of expression,in
R10569 T14174 T14172 arg2Of cells,in
R10570 T14174 T14173 arg1Of cells,nTreg
R10571 T14176 T14175 arg1Of vivo,in
R10572 T14177 T14181 arg1Of or,may
R10573 T14177 T14182 arg1Of or,contribute
R10574 T14178 T14177 arg2Of that,or
R10575 T14180 T14178 arg2Of effects,that
R10576 T14180 T14179 arg1Of effects,additional
R10577 T14182 T14137 arg1Of contribute,But
R10578 T14182 T14138 arg1Of contribute,since
R10579 T14182 T14181 arg2Of contribute,may
R10580 T14182 T14183 arg1Of contribute,to
R10581 T14182 T14187 arg1Of contribute,in
R10582 T14186 T14183 arg2Of drop,to
R10583 T14186 T14184 arg1Of drop,the
R10584 T14186 T14185 arg2Of drop,observed
R10585 T14189 T14187 arg2Of expression,in
R10586 T14189 T14188 arg1Of expression,Foxp3
R10587 T14190 T14191 arg1Of IL-4,has
R10588 T14190 T14193 arg1Of IL-4,been
R10589 T14190 T14194 arg2Of IL-4,shown
R10590 T14190 T14197 arg1Of IL-4,regulate
R10591 T14194 T14191 arg2Of shown,has
R10592 T14194 T14192 arg1Of shown,already
R10593 T14194 T14193 arg2Of shown,been
R10594 T14197 T14194 arg3Of regulate,shown
R10595 T14197 T14195 arg1Of regulate,to
R10596 T14197 T14196 arg1Of regulate,negatively
R10597 T14197 T14214 arg1Of regulate,[
R10598 T14199 T14198 arg1Of development,the
R10599 T14199 T14200 arg1Of development,of
R10600 T14199 T14204 arg1Of development,into
R10601 T14199 T14206 arg1Of development,or
R10602 T14203 T14200 arg2Of cells,of
R10603 T14203 T14201 arg1Of cells,naive
R10604 T14203 T14202 arg1Of cells,T
R10605 T14205 T14204 arg2Of Th1,into
R10606 T14206 T14197 arg2Of or,regulate
R10607 T14210 T14206 arg2Of cells,or
R10608 T14210 T14207 arg1Of cells,the
R10609 T14210 T14208 arg1Of cells,IL-17–producing
R10610 T14210 T14209 arg1Of cells,T
R10611 T14210 T14211 arg1Of cells,(
R10612 T14212 T14211 arg2Of Th17,(
R10613 T14213 T14211 arg3Of ),(
R10614 T14215 T14214 arg2Of "46,47",[
R10615 T14216 T14214 arg3Of ],[
R10616 T14218 T14217 arg1Of effects,Similar
R10617 T14218 T14219 arg1Of effects,have
R10618 T14218 T14220 arg1Of effects,been
R10619 T14218 T14222 arg2Of effects,described
R10620 T14222 T14219 arg2Of described,have
R10621 T14222 T14220 arg2Of described,been
R10622 T14222 T14221 arg1Of described,recently
R10623 T14222 T14223 arg1Of described,for
R10624 T14224 T14223 arg2Of IL-6,for
R10625 T14224 T14225 arg1Of IL-6,","
R10626 T14224 T14226 arg1Of IL-6,which
R10627 T14224 T14228 arg2Of IL-6,combined
R10628 T14224 T14232 arg1Of IL-6,inhibits
R10629 T14224 T14239 arg1Of IL-6,induces
R10630 T14228 T14229 arg1Of combined,with
R10631 T14230 T14229 arg2Of TGF-β,with
R10632 T14232 T14238 arg1Of inhibits,and
R10633 T14234 T14232 arg2Of generation,inhibits
R10634 T14234 T14233 arg1Of generation,the
R10635 T14234 T14235 arg1Of generation,of
R10636 T14237 T14235 arg2Of cells,of
R10637 T14237 T14236 arg1Of cells,iTreg
R10638 T14238 T14227 arg1Of and,","
R10639 T14238 T14228 modOf and,combined
R10640 T14238 T14231 arg1Of and,","
R10641 T14239 T14238 arg2Of induces,and
R10642 T14239 T14245 arg1Of induces,by
R10643 T14240 T14239 arg2Of differentiation,induces
R10644 T14240 T14241 arg1Of differentiation,into
R10645 T14244 T14241 arg2Of cells,into
R10646 T14244 T14242 arg1Of cells,the
R10647 T14244 T14243 arg1Of cells,Th17
R10648 T14248 T14245 arg2Of mechanism,by
R10649 T14248 T14246 arg1Of mechanism,an
R10650 T14248 T14247 arg1Of mechanism,unknown
R10651 T14248 T14249 arg1Of mechanism,[
R10652 T14250 T14249 arg2Of "48,49",[
R10653 T14251 T14249 arg3Of ],[
R10654 T14254 T14253 arg1Of polarization,the
R10655 T14254 T14255 arg1Of polarization,into
R10656 T14254 T14258 arg1Of polarization,is
R10657 T14254 T14260 arg2Of polarization,regulated
R10658 T14257 T14255 arg2Of cells,into
R10659 T14257 T14256 arg1Of cells,iTreg
R10660 T14260 T14252 arg1Of regulated,Thus
R10661 T14260 T14258 arg2Of regulated,is
R10662 T14260 T14259 arg1Of regulated,negatively
R10663 T14264 T14262 arg1Of cytokines,the
R10664 T14264 T14263 arg1Of cytokines,effector
R10665 T14265 T14266 arg1Of IL-4,and
R10666 T14266 T14260 arg1Of and,regulated
R10667 T14266 T14261 arg2Of and,by
R10668 T14266 T14264 arg1Of and,cytokines
R10669 T14267 T14266 arg2Of IL-6,and
R10670 T14268 T14269 arg1Of IL-4,has
R10671 T14268 T14270 arg1Of IL-4,been
R10672 T14268 T14272 arg2Of IL-4,shown
R10673 T14268 T14274 arg1Of IL-4,induce
R10674 T14272 T14269 arg2Of shown,has
R10675 T14272 T14270 arg2Of shown,been
R10676 T14272 T14271 arg1Of shown,previously
R10677 T14274 T14272 arg3Of induce,shown
R10678 T14274 T14273 arg1Of induce,to
R10679 T14274 T14282 arg1Of induce,of
R10680 T14274 T14284 arg1Of induce,[
R10681 T14276 T14274 arg2Of generation,induce
R10682 T14276 T14275 arg1Of generation,the
R10683 T14276 T14277 arg1Of generation,of
R10684 T14280 T14277 arg2Of cells,of
R10685 T14280 T14278 arg1Of cells,FOXP3+
R10686 T14280 T14279 arg1Of cells,Treg
R10687 T14282 T14281 arg1Of of,out
R10688 T14283 T14282 arg2Of CD4+CD25–,of
R10689 T14285 T14284 arg2Of 50,[
R10690 T14286 T14284 arg3Of ],[
R10691 T14289 T14287 arg2Of experiments,In
R10692 T14289 T14288 arg1Of experiments,those
R10693 T14292 T14291 arg1Of concentrations,the
R10694 T14292 T14293 arg1Of concentrations,of
R10695 T14292 T14296 arg1Of concentrations,were
R10696 T14292 T14297 arg1Of concentrations,low
R10697 T14292 T14310 arg1Of concentrations,enhanced
R10698 T14294 T14293 arg2Of IL-4,of
R10699 T14294 T14295 arg2Of IL-4,used
R10700 T14296 T14299 arg1Of were,and
R10701 T14297 T14296 arg2Of low,were
R10702 T14299 T14287 arg1Of and,In
R10703 T14299 T14290 arg1Of and,","
R10704 T14299 T14298 arg1Of and,","
R10705 T14301 T14303 arg1Of we,observed
R10706 T14303 T14300 arg2Of observed,as
R10707 T14303 T14302 arg1Of observed,also
R10708 T14303 T14304 arg1Of observed,","
R10709 T14305 T14303 arg2Of IL-4,observed
R10710 T14305 T14306 arg1Of IL-4,at
R10711 T14308 T14306 arg2Of concentration,at
R10712 T14308 T14307 arg1Of concentration,low
R10713 T14310 T14299 arg2Of enhanced,and
R10714 T14310 T14300 arg1Of enhanced,as
R10715 T14310 T14309 arg1Of enhanced,slightly
R10716 T14312 T14310 arg2Of expression,enhanced
R10717 T14312 T14311 arg1Of expression,FOXP3
R10718 T14316 T14315 arg1Of concentrations,these
R10719 T14316 T14317 arg1Of concentrations,were
R10720 T14316 T14319 arg1Of concentrations,sufficient
R10721 T14317 T14313 arg1Of were,Importantly
R10722 T14317 T14314 arg1Of were,","
R10723 T14317 T14318 arg1Of were,not
R10724 T14319 T14317 arg2Of sufficient,were
R10725 T14321 T14319 arg2Of induce,sufficient
R10726 T14321 T14320 arg1Of induce,to
R10727 T14323 T14321 arg2Of expression,induce
R10728 T14323 T14322 arg1Of expression,GATA3
R10729 T14324 T14325 arg1Of IL-4,may
R10730 T14324 T14326 arg1Of IL-4,favor
R10731 T14324 T14336 arg1Of IL-4,regulate
R10732 T14326 T14334 arg1Of favor,or
R10733 T14327 T14326 arg2Of proliferation,favor
R10734 T14327 T14328 arg1Of proliferation,of
R10735 T14327 T14331 arg1Of proliferation,[
R10736 T14330 T14328 arg2Of cells,of
R10737 T14330 T14329 arg1Of cells,nTreg
R10738 T14332 T14331 arg2Of 47,[
R10739 T14333 T14331 arg3Of ],[
R10740 T14334 T14325 arg2Of or,may
R10741 T14336 T14334 arg2Of regulate,or
R10742 T14336 T14335 arg1Of regulate,directly
R10743 T14336 T14339 arg1Of regulate,in
R10744 T14338 T14336 arg2Of expression,regulate
R10745 T14338 T14337 arg1Of expression,FOXP3
R10746 T14342 T14339 arg2Of fashion,in
R10747 T14342 T14340 arg1Of fashion,a
R10748 T14342 T14341 arg1Of fashion,STAT-dependent
R10749 T14342 T14343 arg1Of fashion,[
R10750 T14344 T14343 arg2Of 51,[
R10751 T14345 T14343 arg3Of ],[
R10752 T14347 T14348 arg1Of IL-13,does
R10753 T14347 T14351 arg1Of IL-13,reduce
R10754 T14347 T14354 arg1Of IL-13,fails
R10755 T14347 T14356 arg1Of IL-13,induce
R10756 T14351 T14348 arg2Of reduce,does
R10757 T14351 T14349 arg1Of reduce,not
R10758 T14351 T14350 arg1Of reduce,effectively
R10759 T14351 T14353 arg1Of reduce,and
R10760 T14352 T14351 arg2Of FOXP3,reduce
R10761 T14353 T14346 arg2Of and,Since
R10762 T14354 T14353 arg2Of fails,and
R10763 T14356 T14354 arg2Of induce,fails
R10764 T14356 T14355 arg1Of induce,to
R10765 T14357 T14356 arg2Of GATA3,induce
R10766 T14359 T14360 arg1Of we,hypothesized
R10767 T14360 T14346 arg1Of hypothesized,Since
R10768 T14360 T14358 arg1Of hypothesized,","
R10769 T14364 T14362 arg1Of inhibition,the
R10770 T14364 T14363 arg1Of inhibition,IL-4–dependent
R10771 T14364 T14365 arg1Of inhibition,of
R10772 T14364 T14367 arg1Of inhibition,could
R10773 T14364 T14368 arg1Of inhibition,be
R10774 T14364 T14369 arg2Of inhibition,mediated
R10775 T14366 T14365 arg2Of FOXP3,of
R10776 T14369 T14360 arg2Of mediated,hypothesized
R10777 T14369 T14361 arg1Of mediated,that
R10778 T14369 T14367 arg2Of mediated,could
R10779 T14369 T14368 arg2Of mediated,be
R10780 T14371 T14369 arg1Of GATA3,mediated
R10781 T14371 T14370 arg2Of GATA3,by
R10782 T14373 T14372 arg2Of fact,In
R10783 T14377 T14375 arg1Of concentrations,GATA3-inducing
R10784 T14377 T14376 arg1Of concentrations,IL-4
R10785 T14377 T14378 arg1Of concentrations,repressed
R10786 T14378 T14372 arg1Of repressed,In
R10787 T14378 T14374 arg1Of repressed,","
R10788 T14378 T14382 arg1Of repressed,","
R10789 T14378 T14383 arg1Of repressed,whereas
R10790 T14381 T14378 arg2Of expression,repressed
R10791 T14381 T14379 arg1Of expression,TGF-β–mediated
R10792 T14381 T14380 arg1Of expression,FOXP3
R10793 T14384 T14387 arg1Of IL-4,as
R10794 T14387 T14385 arg1Of as,as
R10795 T14387 T14386 arg1Of as,well
R10796 T14387 T14390 arg1Of as,were
R10797 T14387 T14391 arg1Of as,intact
R10798 T14389 T14387 arg2Of signaling,as
R10799 T14389 T14388 arg1Of signaling,TGF-β
R10800 T14390 T14383 arg2Of were,whereas
R10801 T14391 T14390 arg2Of intact,were
R10802 T14393 T14392 arg1Of result,This
R10803 T14393 T14394 arg1Of result,suggested
R10804 T14397 T14394 arg2Of mechanism,suggested
R10805 T14397 T14395 arg1Of mechanism,a
R10806 T14397 T14396 arg1Of mechanism,competitive
R10807 T14397 T14398 arg1Of mechanism,between
R10808 T14397 T14404 arg1Of mechanism,in
R10809 T14399 T14400 arg1Of GATA3,and
R10810 T14401 T14400 arg2Of FOXP3,and
R10811 T14403 T14398 arg2Of factors,between
R10812 T14403 T14399 arg1Of factors,GATA3
R10813 T14403 T14401 arg1Of factors,FOXP3
R10814 T14403 T14402 arg1Of factors,transcription
R10815 T14405 T14404 arg2Of determining,in
R10816 T14405 T14408 arg1Of determining,during
R10817 T14407 T14405 arg2Of commitment,determining
R10818 T14407 T14406 arg1Of commitment,lineage
R10819 T14411 T14408 arg2Of phase,during
R10820 T14411 T14409 arg1Of phase,the
R10821 T14411 T14410 arg1Of phase,early
R10822 T14411 T14412 arg1Of phase,of
R10823 T14413 T14412 arg2Of differentiation,of
R10824 T14416 T14417 arg1Of we,investigated
R10825 T14416 T14423 arg1Of we,confirmed
R10826 T14417 T14422 arg1Of investigated,and
R10827 T14419 T14418 arg1Of high,naturally
R10828 T14421 T14417 arg2Of cells,investigated
R10829 T14421 T14419 arg1Of cells,high
R10830 T14421 T14420 arg1Of cells,GATA3-expressing
R10831 T14422 T14414 arg1Of and,Accordingly
R10832 T14422 T14415 arg1Of and,","
R10833 T14423 T14422 arg2Of confirmed,and
R10834 T14425 T14423 arg2Of absence,confirmed
R10835 T14425 T14424 arg1Of absence,the
R10836 T14425 T14426 arg1Of absence,of
R10837 T14427 T14426 arg2Of FOXP3,of
R10838 T14429 T14428 arg1Of transduction,Protein
R10839 T14429 T14430 arg1Of transduction,of
R10840 T14429 T14432 arg1Of transduction,into
R10841 T14429 T14436 arg1Of transduction,inhibited
R10842 T14431 T14430 arg2Of GATA3,of
R10843 T14435 T14432 arg2Of cells,into
R10844 T14435 T14433 arg1Of cells,naive
R10845 T14435 T14434 arg1Of cells,T
R10846 T14438 T14436 arg2Of induction,inhibited
R10847 T14438 T14437 arg1Of induction,FOXP3
R10848 T14438 T14439 arg1Of induction,in
R10849 T14446 T14439 arg2Of cells,in
R10850 T14446 T14440 arg1Of cells,human
R10851 T14446 T14441 arg1Of cells,","
R10852 T14446 T14442 arg1Of cells,differentiating
R10853 T14446 T14443 arg1Of cells,","
R10854 T14446 T14444 arg1Of cells,naive
R10855 T14446 T14445 arg1Of cells,T
R10856 T14449 T14447 arg1Of effect,This
R10857 T14449 T14448 arg1Of effect,inhibitory
R10858 T14449 T14450 arg1Of effect,of
R10859 T14449 T14452 arg1Of effect,was
R10860 T14449 T14454 arg2Of effect,confirmed
R10861 T14449 T14460 arg1Of effect,expressing
R10862 T14451 T14450 arg2Of GATA3,of
R10863 T14454 T14452 arg2Of confirmed,was
R10864 T14454 T14453 arg1Of confirmed,further
R10865 T14454 T14455 arg1Of confirmed,in
R10866 T14454 T14459 arg1Of confirmed,","
R10867 T14454 T14460 modOf confirmed,expressing
R10868 T14458 T14455 arg2Of mice,in
R10869 T14458 T14456 arg1Of mice,BALB/c
R10870 T14458 T14457 arg1Of mice,transgenic
R10871 T14460 T14462 arg1Of expressing,in
R10872 T14461 T14460 arg2Of GATA3,expressing
R10873 T14464 T14462 arg2Of cells,in
R10874 T14464 T14463 arg1Of cells,T
R10875 T14464 T14465 arg1Of cells,(
R10876 T14466 T14465 arg2Of DO11.10,(
R10877 T14466 T14467 arg1Of DO11.10,:
R10878 T14470 T14467 arg2Of mice,:
R10879 T14470 T14468 arg1Of mice,CD2-GATA3
R10880 T14470 T14469 arg1Of mice,transgenic
R10881 T14471 T14465 arg3Of ),(
R10882 T14473 T14472 arg2Of line,In
R10883 T14473 T14474 arg1Of line,with
R10884 T14477 T14474 arg2Of overexpression,with
R10885 T14477 T14475 arg1Of overexpression,the
R10886 T14477 T14476 arg1Of overexpression,transient
R10887 T14477 T14478 arg1Of overexpression,of
R10888 T14477 T14480 arg1Of overexpression,in
R10889 T14479 T14478 arg2Of GATA3,of
R10890 T14483 T14480 arg2Of cells,in
R10891 T14483 T14481 arg1Of cells,human
R10892 T14483 T14482 arg1Of cells,T
R10893 T14485 T14486 arg1Of cells,of
R10894 T14485 T14489 arg1Of cells,failed
R10895 T14485 T14491 arg1Of cells,induce
R10896 T14488 T14486 arg2Of mice,of
R10897 T14488 T14487 arg1Of mice,these
R10898 T14489 T14472 arg1Of failed,In
R10899 T14489 T14484 arg1Of failed,","
R10900 T14491 T14489 arg2Of induce,failed
R10901 T14491 T14490 arg1Of induce,to
R10902 T14491 T14494 arg1Of induce,upon
R10903 T14493 T14491 arg2Of expression,induce
R10904 T14493 T14492 arg1Of expression,FOXP3
R10905 T14495 T14494 arg2Of exposure,upon
R10906 T14495 T14496 arg1Of exposure,with
R10907 T14497 T14496 arg2Of antigen,with
R10908 T14497 T14498 arg1Of antigen,in
R10909 T14500 T14498 arg2Of presence,in
R10910 T14500 T14499 arg1Of presence,the
R10911 T14500 T14501 arg1Of presence,of
R10912 T14502 T14501 arg2Of TGF-β,of
R10913 T14506 T14507 arg1Of DO11.10,:
R10914 T14508 T14507 arg2Of CD2-GATA3,:
R10915 T14509 T14505 arg1Of mice,the
R10916 T14509 T14506 arg1Of mice,DO11.10
R10917 T14509 T14508 arg1Of mice,CD2-GATA3
R10918 T14509 T14510 arg1Of mice,do
R10919 T14509 T14511 arg1Of mice,have
R10920 T14511 T14503 arg1Of have,Strikingly
R10921 T14511 T14504 arg1Of have,","
R10922 T14511 T14510 arg2Of have,do
R10923 T14514 T14511 arg2Of cells,have
R10924 T14514 T14512 arg1Of cells,peripheral
R10925 T14514 T14513 arg1Of cells,FOXP3+
R10926 T14514 T14515 arg1Of cells,","
R10927 T14514 T14516 arg1Of cells,which
R10928 T14514 T14518 arg1Of cells,displayed
R10929 T14518 T14517 arg1Of displayed,however
R10930 T14518 T14526 arg1Of displayed,compared
R10931 T14523 T14519 arg1Of %,a
R10932 T14523 T14520 arg1Of %,10
R10933 T14523 T14521 arg1Of %,%
R10934 T14523 T14522 arg1Of %,–25
R10935 T14524 T14523 arg1Of lower,%
R10936 T14525 T14518 arg2Of frequency,displayed
R10937 T14525 T14524 arg1Of frequency,lower
R10938 T14527 T14526 arg2Of to,compared
R10939 T14530 T14527 arg2Of mice,to
R10940 T14530 T14528 arg1Of mice,wild-type
R10941 T14530 T14529 arg1Of mice,DO11.10
R10942 T14532 T14533 arg1Of GATA3,restrains
R10943 T14533 T14531 arg1Of restrains,Thus
R10944 T14535 T14533 arg2Of development,restrains
R10945 T14535 T14534 arg1Of development,the
R10946 T14535 T14536 arg1Of development,of
R10947 T14539 T14536 arg2Of subsets,of
R10948 T14539 T14537 arg1Of subsets,certain
R10949 T14539 T14538 arg1Of subsets,Treg
R10950 T14539 T14540 arg1Of subsets,","
R10951 T14543 T14544 arg1Of inducible,","
R10952 T14545 T14544 arg2Of peripheral,","
R10953 T14546 T14542 arg1Of population,the
R10954 T14546 T14543 arg1Of population,inducible
R10955 T14546 T14545 arg1Of population,peripheral
R10956 T14546 T14547 arg1Of population,and
R10957 T14547 T14540 arg2Of and,","
R10958 T14547 T14541 arg1Of and,presumably
R10959 T14549 T14547 arg2Of those,and
R10960 T14549 T14548 arg1Of those,not
R10961 T14549 T14550 arg1Of those,of
R10962 T14552 T14550 arg2Of origin,of
R10963 T14552 T14551 arg1Of origin,thymic
R10964 T14555 T14553 arg1Of cells,Thymic
R10965 T14555 T14554 arg1Of cells,T
R10966 T14555 T14556 arg1Of cells,undergo
R10967 T14560 T14556 arg2Of process,undergo
R10968 T14560 T14557 arg1Of process,a
R10969 T14560 T14558 arg1Of process,different
R10970 T14560 T14559 arg1Of process,maturation
R10971 T14560 T14561 arg1Of process,","
R10972 T14560 T14562 arg1Of process,which
R10973 T14560 T14563 arg1Of process,may
R10974 T14560 T14564 arg1Of process,explain
R10975 T14564 T14563 arg2Of explain,may
R10976 T14564 T14572 arg1Of explain,[
R10977 T14566 T14564 arg2Of insensitivity,explain
R10978 T14566 T14565 arg1Of insensitivity,the
R10979 T14566 T14567 arg1Of insensitivity,of
R10980 T14566 T14569 arg1Of insensitivity,to
R10981 T14568 T14567 arg2Of nTreg,of
R10982 T14571 T14569 arg2Of overexpression,to
R10983 T14571 T14570 arg1Of overexpression,GATA3
R10984 T14573 T14572 arg2Of 52,[
R10985 T14574 T14572 arg3Of ],[
R10986 T14575 T14577 arg1Of In,to
R10987 T14576 T14575 arg2Of contrast,In
R10988 T14579 T14580 arg1Of Th2-differentiating,and
R10989 T14581 T14580 arg2Of iTreg-inhibiting,and
R10990 T14582 T14575 arg3Of function,In
R10991 T14582 T14578 arg1Of function,the
R10992 T14582 T14579 arg1Of function,Th2-differentiating
R10993 T14582 T14581 arg1Of function,iTreg-inhibiting
R10994 T14582 T14583 arg1Of function,of
R10995 T14582 T14585 arg1Of function,in
R10996 T14584 T14583 arg2Of GATA3,of
R10997 T14588 T14585 arg2Of cells,in
R10998 T14588 T14586 arg1Of cells,peripheral
R10999 T14588 T14587 arg1Of cells,T
R11000 T14590 T14591 arg1Of GATA3,acts
R11001 T14590 T14620 arg1Of GATA3,participates
R11002 T14590 T14627 arg1Of GATA3,regulate
R11003 T14591 T14592 arg1Of acts,in
R11004 T14591 T14619 arg1Of acts,or
R11005 T14594 T14592 arg2Of thymus,in
R11006 T14594 T14593 arg1Of thymus,the
R11007 T14594 T14595 arg1Of thymus,together
R11008 T14594 T14609 arg1Of thymus,in
R11009 T14595 T14596 arg1Of together,with
R11010 T14599 T14596 arg2Of factors,with
R11011 T14599 T14597 arg1Of factors,other
R11012 T14599 T14598 arg1Of factors,transcription
R11013 T14599 T14601 arg1Of factors,as
R11014 T14599 T14606 arg1Of factors,(
R11015 T14601 T14600 arg1Of as,such
R11016 T14603 T14601 arg2Of Repressor,as
R11017 T14603 T14602 arg1Of Repressor,the
R11018 T14603 T14604 arg1Of Repressor,of
R11019 T14605 T14604 arg2Of GATA3,of
R11020 T14607 T14606 arg2Of ROG,(
R11021 T14608 T14606 arg3Of ),(
R11022 T14612 T14609 arg2Of process,in
R11023 T14612 T14610 arg1Of process,the
R11024 T14612 T14611 arg1Of process,differentiation
R11025 T14612 T14613 arg1Of process,toward
R11026 T14615 T14613 arg2Of cells,toward
R11027 T14615 T14614 arg1Of cells,CD8
R11028 T14615 T14616 arg1Of cells,[
R11029 T14617 T14616 arg2Of "53,54",[
R11030 T14618 T14616 arg3Of ],[
R11031 T14619 T14575 arg1Of or,In
R11032 T14619 T14589 arg1Of or,","
R11033 T14620 T14619 arg2Of participates,or
R11034 T14620 T14621 arg1Of participates,in
R11035 T14620 T14626 modOf participates,to
R11036 T14625 T14621 arg2Of network,in
R11037 T14625 T14622 arg1Of network,complex
R11038 T14625 T14623 arg1Of network,transcriptional
R11039 T14625 T14624 arg1Of network,feedback
R11040 T14627 T14626 arg1Of regulate,to
R11041 T14629 T14627 arg2Of differentiation,regulate
R11042 T14629 T14628 arg1Of differentiation,sympathoadrenal
R11043 T14629 T14630 arg1Of differentiation,[
R11044 T14631 T14630 arg2Of 55,[
R11045 T14632 T14630 arg3Of ],[
R11046 T14636 T14635 arg1Of role,the
R11047 T14636 T14637 arg1Of role,of
R11048 T14636 T14639 arg1Of role,appears
R11049 T14636 T14641 arg1Of role,be
R11050 T14636 T14643 arg1Of role,specific
R11051 T14636 T14645 arg1Of role,can
R11052 T14636 T14647 arg1Of role,be
R11053 T14636 T14648 arg2Of role,generalized
R11054 T14638 T14637 arg2Of GATA3,of
R11055 T14639 T14644 arg1Of appears,and
R11056 T14641 T14639 arg2Of be,appears
R11057 T14641 T14640 arg1Of be,to
R11058 T14643 T14641 arg2Of specific,be
R11059 T14643 T14642 arg1Of specific,tissue
R11060 T14644 T14633 arg1Of and,Therefore
R11061 T14644 T14634 arg1Of and,","
R11062 T14648 T14644 arg2Of generalized,and
R11063 T14648 T14645 arg2Of generalized,can
R11064 T14648 T14646 arg1Of generalized,not
R11065 T14648 T14647 arg2Of generalized,be
R11066 T14650 T14649 arg1Of study,Our
R11067 T14650 T14651 arg1Of study,demonstrates
R11068 T14653 T14654 arg1Of GATA3,repressed
R11069 T14653 T14659 arg1Of GATA3,binding
R11070 T14654 T14651 arg2Of repressed,demonstrates
R11071 T14654 T14652 arg1Of repressed,that
R11072 T14654 T14657 arg1Of repressed,directly
R11073 T14654 T14658 arg1Of repressed,by
R11074 T14656 T14654 arg2Of expression,repressed
R11075 T14656 T14655 arg1Of expression,FOXP3
R11076 T14659 T14658 arg2Of binding,by
R11077 T14659 T14660 arg1Of binding,to
R11078 T14664 T14660 arg2Of region,to
R11079 T14664 T14661 arg1Of region,the
R11080 T14664 T14662 arg1Of region,FOXP3
R11081 T14664 T14663 arg1Of region,promoter
R11082 T14667 T14665 arg1Of GATA-site,A
R11083 T14667 T14666 arg1Of GATA-site,palindromic
R11084 T14667 T14668 arg1Of GATA-site,is
R11085 T14667 T14669 arg1Of GATA-site,located
R11086 T14669 T14668 arg2Of located,is
R11087 T14669 T14671 arg1Of located,bp
R11088 T14671 T14670 arg1Of bp,303
R11089 T14671 T14672 arg1Of bp,upstream
R11090 T14672 T14673 arg1Of upstream,of
R11091 T14675 T14673 arg2Of TSS,of
R11092 T14675 T14674 arg1Of TSS,the
R11093 T14675 T14676 arg1Of TSS,in
R11094 T14679 T14678 arg1Of conserved,highly
R11095 T14680 T14676 arg2Of region,in
R11096 T14680 T14677 arg1Of region,a
R11097 T14680 T14679 arg1Of region,conserved
R11098 T14680 T14681 arg1Of region,","
R11099 T14680 T14682 arg1Of region,which
R11100 T14680 T14686 arg2Of region,identified
R11101 T14683 T14684 arg1Of we,have
R11102 T14683 T14686 arg1Of we,identified
R11103 T14686 T14684 arg2Of identified,have
R11104 T14686 T14685 arg1Of identified,previously
R11105 T14686 T14687 arg1Of identified,as
R11106 T14686 T14691 arg1Of identified,[
R11107 T14690 T14687 arg2Of promoter,as
R11108 T14690 T14688 arg1Of promoter,the
R11109 T14690 T14689 arg1Of promoter,FOXP3
R11110 T14692 T14691 arg2Of 24,[
R11111 T14693 T14691 arg3Of ],[
R11112 T14695 T14694 arg1Of mutation,Site-specific
R11113 T14695 T14696 arg1Of mutation,of
R11114 T14695 T14699 arg1Of mutation,increased
R11115 T14698 T14696 arg2Of site,of
R11116 T14698 T14697 arg1Of site,this
R11117 T14699 T14706 arg1Of increased,","
R11118 T14699 T14708 modOf increased,revealing
R11119 T14701 T14699 arg2Of activity,increased
R11120 T14701 T14700 arg1Of activity,the
R11121 T14701 T14702 arg1Of activity,of
R11122 T14705 T14702 arg2Of constructs,of
R11123 T14705 T14703 arg1Of constructs,the
R11124 T14705 T14704 arg1Of constructs,promoter
R11125 T14708 T14707 arg1Of revealing,thus
R11126 T14711 T14708 arg2Of nature,revealing
R11127 T14711 T14709 arg1Of nature,the
R11128 T14711 T14710 arg1Of nature,repressive
R11129 T14711 T14712 arg1Of nature,of
R11130 T14715 T14712 arg2Of element,of
R11131 T14715 T14713 arg1Of element,this
R11132 T14715 T14714 arg1Of element,GATA
R11133 T14715 T14716 arg1Of element,in
R11134 T14719 T14716 arg2Of cells,in
R11135 T14719 T14717 arg1Of cells,memory
R11136 T14719 T14718 arg1Of cells,T
R11137 T14719 T14720 arg1Of cells,","
R11138 T14719 T14721 arg1Of cells,which
R11139 T14719 T14723 arg1Of cells,express
R11140 T14723 T14722 arg1Of express,naturally
R11141 T14723 T14725 arg1Of express,","
R11142 T14723 T14726 arg1Of express,whereas
R11143 T14724 T14723 arg2Of GATA3,express
R11144 T14728 T14727 arg1Of difference,no
R11145 T14728 T14729 arg1Of difference,was
R11146 T14728 T14730 arg2Of difference,seen
R11147 T14730 T14726 arg2Of seen,whereas
R11148 T14730 T14729 arg2Of seen,was
R11149 T14730 T14731 arg1Of seen,in
R11150 T14734 T14731 arg2Of cells,in
R11151 T14734 T14732 arg1Of cells,naive
R11152 T14734 T14733 arg1Of cells,T
R11153 T14734 T14735 arg1Of cells,","
R11154 T14734 T14736 arg1Of cells,which
R11155 T14734 T14737 arg1Of cells,do
R11156 T14734 T14739 arg1Of cells,express
R11157 T14739 T14737 arg2Of express,do
R11158 T14739 T14738 arg1Of express,not
R11159 T14740 T14739 arg2Of GATA3,express
R11160 T14744 T14741 arg1Of element,This
R11161 T14744 T14742 arg1Of element,palindromic
R11162 T14744 T14743 arg1Of element,GATA
R11163 T14744 T14745 arg1Of element,is
R11164 T14744 T14746 arg2Of element,bound
R11165 T14746 T14745 arg2Of bound,is
R11166 T14746 T14750 arg1Of bound,as
R11167 T14749 T14746 arg1Of protein,bound
R11168 T14749 T14747 arg2Of protein,by
R11169 T14749 T14748 arg1Of protein,GATA3
R11170 T14751 T14750 arg2Of proven,as
R11171 T14751 T14752 arg1Of proven,with
R11172 T14754 T14752 arg2Of experiments,with
R11173 T14754 T14753 arg1Of experiments,pull-down
R11174 T14757 T14758 arg1Of it,is
R11175 T14757 T14759 arg2Of it,shown
R11176 T14759 T14755 arg1Of shown,Furthermore
R11177 T14759 T14756 arg1Of shown,","
R11178 T14759 T14758 arg2Of shown,is
R11179 T14761 T14759 arg1Of ChIP,shown
R11180 T14761 T14760 arg2Of ChIP,by
R11181 T14763 T14764 arg1Of GATA3,binds
R11182 T14764 T14759 arg3Of binds,shown
R11183 T14764 T14762 arg1Of binds,that
R11184 T14764 T14767 arg1Of binds,also
R11185 T14764 T14768 arg1Of binds,in
R11186 T14766 T14764 arg2Of element,binds
R11187 T14766 T14765 arg1Of element,this
R11188 T14770 T14768 arg2Of cells,in
R11189 T14770 T14769 arg1Of cells,intact
R11190 T14771 T14772 arg1Of It,is
R11191 T14771 T14773 arg2Of It,known
R11192 T14773 T14772 arg2Of known,is
R11193 T14775 T14776 arg1Of GATA3,can
R11194 T14775 T14777 arg1Of GATA3,induce
R11195 T14775 T14788 arg1Of GATA3,transactivating
R11196 T14775 T14797 arg2Of GATA3,shown
R11197 T14775 T14803 arg1Of GATA3,acts
R11198 T14777 T14776 arg2Of induce,can
R11199 T14777 T14782 arg1Of induce,[
R11200 T14777 T14785 arg1Of induce,","
R11201 T14777 T14786 arg1Of induce,by
R11202 T14777 T14793 arg1Of induce,","
R11203 T14777 T14794 arg1Of induce,or
R11204 T14777 T14795 arg1Of induce,","
R11205 T14778 T14777 arg2Of transcription,induce
R11206 T14778 T14779 arg1Of transcription,by
R11207 T14780 T14779 arg2Of chromatin,by
R11208 T14780 T14781 arg1Of chromatin,remodeling
R11209 T14783 T14782 arg2Of 56,[
R11210 T14784 T14782 arg3Of ],[
R11211 T14788 T14786 arg2Of transactivating,by
R11212 T14788 T14787 arg1Of transactivating,directly
R11213 T14789 T14788 arg2Of promoters,transactivating
R11214 T14789 T14790 arg1Of promoters,[
R11215 T14791 T14790 arg2Of 36,[
R11216 T14792 T14790 arg3Of ],[
R11217 T14795 T14773 arg3Of ",",known
R11218 T14795 T14774 arg1Of ",",that
R11219 T14797 T14796 arg2Of shown,as
R11220 T14797 T14798 arg1Of shown,in
R11221 T14801 T14798 arg2Of study,in
R11222 T14801 T14799 arg1Of study,the
R11223 T14801 T14800 arg1Of study,current
R11224 T14803 T14795 arg2Of acts,","
R11225 T14803 T14796 arg1Of acts,as
R11226 T14803 T14802 arg1Of acts,","
R11227 T14803 T14804 arg1Of acts,as
R11228 T14806 T14804 arg2Of repressor,as
R11229 T14806 T14805 arg1Of repressor,a
R11230 T14806 T14807 arg1Of repressor,of
R11231 T14809 T14807 arg2Of expression,of
R11232 T14809 T14808 arg1Of expression,gene
R11233 T14809 T14810 arg1Of expression,[
R11234 T14811 T14810 arg2Of "21,57–59",[
R11235 T14812 T14810 arg3Of ],[
R11236 T14816 T14814 arg1Of expression,keeping
R11237 T14816 T14815 arg1Of expression,GATA3
R11238 T14816 T14817 arg1Of expression,low
R11239 T14816 T14818 arg1Of expression,might
R11240 T14816 T14819 arg1Of expression,be
R11241 T14816 T14820 arg2Of expression,required
R11242 T14816 T14822 arg1Of expression,induce
R11243 T14820 T14813 arg1Of required,Therefore
R11244 T14820 T14818 arg2Of required,might
R11245 T14820 T14819 arg2Of required,be
R11246 T14822 T14820 arg3Of induce,required
R11247 T14822 T14821 arg1Of induce,to
R11248 T14827 T14822 arg2Of generation,induce
R11249 T14827 T14823 arg1Of generation,efficient
R11250 T14827 T14824 arg1Of generation,FOXP3+
R11251 T14827 T14825 arg1Of generation,iTreg
R11252 T14827 T14826 arg1Of generation,cell
R11253 T14830 T14828 arg1Of interactions,The
R11254 T14830 T14829 arg1Of interactions,molecular
R11255 T14830 T14831 arg1Of interactions,enabling
R11256 T14830 T14836 arg1Of interactions,are
R11257 T14830 T14838 arg2Of interactions,identified
R11258 T14832 T14831 arg2Of GATA3,enabling
R11259 T14832 T14833 modOf GATA3,to
R11260 T14834 T14833 arg1Of inhibit,to
R11261 T14835 T14834 arg2Of FOXP3,inhibit
R11262 T14838 T14836 arg2Of identified,are
R11263 T14838 T14837 arg1Of identified,not
R11264 T14838 T14839 arg1Of identified,yet
R11265 T14838 T14841 arg1Of identified,but
R11266 T14841 T14840 arg1Of but,","
R11267 T14844 T14842 arg1Of site,the
R11268 T14844 T14843 arg1Of site,GATA-binding
R11269 T14844 T14845 arg1Of site,is
R11270 T14844 T14846 arg2Of site,located
R11271 T14844 T14851 arg1Of site,inducing
R11272 T14846 T14845 arg2Of located,is
R11273 T14846 T14847 arg1Of located,adjacent
R11274 T14846 T14848 arg1Of located,to
R11275 T14846 T14850 arg1Of located,","
R11276 T14846 T14851 modOf located,inducing
R11277 T14846 T14862 arg1Of located,and
R11278 T14849 T14848 arg2Of positive,to
R11279 T14852 T14851 arg2Of sites,inducing
R11280 T14852 T14853 arg1Of sites,","
R11281 T14852 T14854 arg2Of sites,composed
R11282 T14854 T14855 arg1Of composed,of
R11283 T14857 T14855 arg2Of sites,of
R11284 T14857 T14856 arg1Of sites,AP-1-NFATc2
R11285 T14857 T14858 arg1Of sites,[
R11286 T14859 T14858 arg2Of 24,[
R11287 T14860 T14858 arg3Of ],[
R11288 T14862 T14841 arg2Of and,but
R11289 T14862 T14861 arg1Of and,","
R11290 T14863 T14864 arg1Of GATA3,may
R11291 T14863 T14865 arg1Of GATA3,compete
R11292 T14865 T14862 arg2Of compete,and
R11293 T14865 T14864 arg2Of compete,may
R11294 T14865 T14866 arg1Of compete,with
R11295 T14868 T14866 arg2Of binding,with
R11296 T14868 T14867 arg1Of binding,the
R11297 T14868 T14869 arg1Of binding,of
R11298 T14868 T14871 arg1Of binding,to
R11299 T14870 T14869 arg2Of AP-1/NFAT,of
R11300 T14873 T14871 arg2Of promoter,to
R11301 T14873 T14872 arg1Of promoter,the
R11302 T14873 T14874 arg1Of promoter,(
R11303 T14876 T14874 arg2Of observations,(
R11304 T14876 T14875 arg1Of observations,unpublished
R11305 T14877 T14874 arg3Of ),(
R11306 T14879 T14878 arg2Of summary,In
R11307 T14881 T14882 arg1Of we,demonstrated
R11308 T14882 T14878 arg1Of demonstrated,In
R11309 T14882 T14880 arg1Of demonstrated,","
R11310 T14884 T14885 arg1Of FOXP3,is
R11311 T14884 T14887 arg2Of FOXP3,regulated
R11312 T14887 T14882 arg2Of regulated,demonstrated
R11313 T14887 T14883 arg1Of regulated,that
R11314 T14887 T14885 arg2Of regulated,is
R11315 T14887 T14886 arg1Of regulated,negatively
R11316 T14889 T14887 arg1Of cytokines,regulated
R11317 T14889 T14888 arg2Of cytokines,by
R11318 T14889 T14891 arg1Of cytokines,as
R11319 T14891 T14890 arg1Of as,such
R11320 T14892 T14891 arg2Of IL-4,as
R11321 T14893 T14894 arg1Of GATA3,acts
R11322 T14894 T14895 arg1Of acts,as
R11323 T14894 T14910 arg1Of acts,in
R11324 T14894 T14914 arg1Of acts,by
R11325 T14895 T14909 arg1Of as,as
R11326 T14897 T14895 arg2Of inhibitor,as
R11327 T14897 T14896 arg1Of inhibitor,an
R11328 T14897 T14898 arg1Of inhibitor,of
R11329 T14900 T14898 arg2Of expression,of
R11330 T14900 T14899 arg1Of expression,FOXP3
R11331 T14900 T14901 arg1Of expression,in
R11332 T14905 T14901 arg2Of differentiation,in
R11333 T14905 T14902 arg1Of differentiation,early
R11334 T14905 T14903 arg1Of differentiation,T
R11335 T14905 T14904 arg1Of differentiation,cell
R11336 T14909 T14906 arg1Of as,","
R11337 T14909 T14907 arg1Of as,as
R11338 T14909 T14908 arg1Of as,well
R11339 T14910 T14909 arg2Of in,as
R11340 T14913 T14910 arg2Of cells,in
R11341 T14913 T14911 arg2Of cells,differentiated
R11342 T14913 T14912 arg1Of cells,Th2
R11343 T14916 T14917 arg1Of binding,and
R11344 T14917 T14914 arg2Of and,by
R11345 T14917 T14915 arg1Of and,directly
R11346 T14918 T14917 arg2Of repressing,and
R11347 T14921 T14916 arg2Of promoter,binding
R11348 T14921 T14918 arg2Of promoter,repressing
R11349 T14921 T14919 arg1Of promoter,the
R11350 T14921 T14920 arg1Of promoter,FOXP3
R11351 T14922 T14924 arg1Of We,describe
R11352 T14922 T14935 arg1Of We,repressing
R11353 T14924 T14923 arg1Of describe,therefore
R11354 T14924 T14934 arg1Of describe,by
R11355 T14927 T14924 arg2Of mechanism,describe
R11356 T14927 T14925 arg1Of mechanism,a
R11357 T14927 T14926 arg1Of mechanism,new
R11358 T14927 T14928 arg1Of mechanism,of
R11359 T14933 T14928 arg2Of induction,of
R11360 T14933 T14929 arg1Of induction,how
R11361 T14933 T14930 arg1Of induction,Il-4
R11362 T14933 T14931 arg1Of induction,avoids
R11363 T14933 T14932 arg1Of induction,tolerance
R11364 T14935 T14934 arg2Of repressing,by
R11365 T14937 T14935 arg2Of expression,repressing
R11366 T14937 T14936 arg1Of expression,FOXP3
R11367 T14939 T14938 arg1Of findings,These
R11368 T14939 T14940 arg1Of findings,will
R11369 T14939 T14941 arg1Of findings,give
R11370 T14941 T14940 arg2Of give,will
R11371 T14941 T14944 arg1Of give,toward
R11372 T14943 T14941 arg2Of perspectives,give
R11373 T14943 T14942 arg1Of perspectives,new
R11374 T14945 T14944 arg2Of understanding,toward
R11375 T14945 T14958 arg1Of understanding,","
R11376 T14945 T14960 arg1Of understanding,in
R11377 T14947 T14945 arg2Of mechanisms,understanding
R11378 T14947 T14946 arg1Of mechanisms,molecular
R11379 T14947 T14948 arg1Of mechanisms,of
R11380 T14950 T14949 arg1Of induction,iTreg
R11381 T14950 T14951 arg1Of induction,and
R11382 T14951 T14948 arg2Of and,of
R11383 T14953 T14951 arg2Of pathways,and
R11384 T14953 T14952 arg1Of pathways,thus
R11385 T14953 T14954 arg1Of pathways,of
R11386 T14957 T14954 arg2Of induction,of
R11387 T14957 T14955 arg1Of induction,peripheral
R11388 T14957 T14956 arg1Of induction,tolerance
R11389 T14960 T14959 arg1Of in,particularly
R11390 T14961 T14962 arg1Of allergy,and
R11391 T14962 T14960 arg2Of and,in
R11392 T14963 T14962 arg2Of asthma,and
R13418 T17781 T17779 arg1Of mice,Normal
R13419 T17781 T17780 arg1Of mice,B6
R13420 T17781 T17782 arg1Of mice,were
R13421 T17781 T17783 arg2Of mice,purchased
R13423 T17783 T17782 arg2Of purchased,were
R13428 T17790 T17788 arg2Of Harbor,(
R13429 T17790 T17789 arg1Of Harbor,Bar
R13430 T17790 T17791 arg1Of Harbor,","
R13431 T17792 T17791 arg2Of Maine,","
R13432 T17793 T17788 arg3Of ),(
R13433 T17796 T17794 arg1Of mice,Transgenic
R13434 T17796 T17795 arg1Of mice,DO11.10
R13435 T17796 T17797 arg1Of mice,","
R13436 T17796 T17798 arg1Of mice,expressing
R13437 T17796 T17812 arg1Of mice,were
R13438 T17796 T17813 arg2Of mice,backcrossed
R13439 T17802 T17798 arg2Of receptor,expressing
R13440 T17802 T17799 arg1Of receptor,a
R13441 T17802 T17800 arg1Of receptor,T
R13442 T17802 T17801 arg1Of receptor,cell
R13443 T17802 T17803 arg1Of receptor,for
R13444 T17805 T17803 arg2Of peptide,for
R13445 T17805 T17804 arg1Of peptide,OVA323–339
R13446 T17805 T17806 arg1Of peptide,in
R13447 T17808 T17806 arg2Of context,in
R13448 T17808 T17807 arg1Of context,the
R13449 T17808 T17809 arg1Of context,of
R13450 T17810 T17809 arg2Of H-2d,of
R13451 T17813 T17811 arg1Of backcrossed,","
R13452 T17813 T17812 arg2Of backcrossed,were
R13453 T17813 T17814 arg1Of backcrossed,with
R13454 T17813 T17830 arg1Of backcrossed,[
R13455 T17813 T17833 arg1Of backcrossed,","
R13456 T17813 T17834 modOf backcrossed,resulting
R13457 T17815 T17814 arg2Of mice,with
R13458 T17815 T17816 arg1Of mice,expressing
R13459 T17815 T17818 arg1Of mice,","
R13460 T17815 T17819 arg2Of mice,driven
R13461 T17817 T17816 arg2Of GATA-3,expressing
R13462 T17826 T17819 arg1Of region,driven
R13463 T17826 T17820 arg2Of region,by
R13464 T17826 T17821 arg1Of region,the
R13465 T17826 T17822 arg1Of region,human
R13466 T17826 T17823 arg1Of region,CD2
R13467 T17826 T17824 arg1Of region,locus
R13468 T17826 T17825 arg1Of region,control
R13469 T17826 T17827 arg1Of region,(
R13470 T17828 T17827 arg2Of CD2-GATA3,(
R13471 T17829 T17827 arg3Of ),(
R13472 T17831 T17830 arg2Of 22,[
R13473 T17832 T17830 arg3Of ],[
R13474 T17834 T17835 arg1Of resulting,in
R13475 T17837 T17835 arg2Of mice,in
R13476 T17837 T17836 arg1Of mice,DO11.10xCD2-GATA3
R13477 T17838 T17839 arg2Of Mice,used
R13478 T17838 T17842 arg1Of Mice,were
R13479 T17838 T17843 arg2Of Mice,backcrossed
R13480 T17838 T17855 arg2Of Mice,used
R13481 T17839 T17840 arg1Of used,for
R13482 T17841 T17840 arg2Of experiments,for
R13483 T17843 T17844 arg1Of backcrossed,on
R13484 T17843 T17854 arg1Of backcrossed,and
R13485 T17847 T17844 arg2Of background,on
R13486 T17847 T17845 arg1Of background,a
R13487 T17847 T17846 arg1Of background,BALB/c
R13488 T17847 T17848 arg1Of background,for
R13489 T17850 T17848 arg2Of minimum,for
R13490 T17850 T17849 arg1Of minimum,a
R13491 T17850 T17851 arg1Of minimum,of
R13492 T17853 T17851 arg2Of generations,of
R13493 T17853 T17852 arg1Of generations,eight
R13494 T17854 T17842 arg2Of and,were
R13495 T17855 T17854 arg2Of used,and
R13496 T17855 T17856 arg1Of used,at
R13497 T17858 T17856 arg2Of age,at
R13498 T17858 T17857 arg1Of age,an
R13499 T17858 T17859 arg1Of age,of
R13500 T17861 T17859 arg2Of wk,of
R13501 T17861 T17860 arg1Of wk,8–12
R13502 T17862 T17863 arg1Of Mice,were
R13503 T17862 T17864 arg2Of Mice,housed
R13504 T17864 T17863 arg2Of housed,were
R13505 T17864 T17865 arg1Of housed,under
R13506 T17864 T17869 arg1Of housed,and
R13507 T17868 T17865 arg2Of conditions,under
R13508 T17868 T17866 arg1Of conditions,specific
R13509 T17868 T17867 arg1Of conditions,pathogen-free
R13510 T17872 T17870 arg1Of studies,all
R13511 T17872 T17871 arg1Of studies,animal
R13512 T17872 T17873 arg1Of studies,were
R13513 T17872 T17874 arg2Of studies,performed
R13514 T17874 T17869 arg2Of performed,and
R13515 T17874 T17873 arg2Of performed,were
R13516 T17874 T17875 arg1Of performed,according
R13517 T17876 T17875 arg2Of to,according
R13518 T17877 T17878 arg1Of institutional,and
R13519 T17879 T17878 arg2Of state,and
R13520 T17880 T17876 arg2Of guidelines,to
R13521 T17880 T17877 arg1Of guidelines,institutional
R13522 T17880 T17879 arg1Of guidelines,state
R13649 T18081 T18082 arg1Of Isolation,of
R13650 T18085 T18082 arg2Of cells,of
R13651 T18085 T18083 arg1Of cells,CD4+
R13652 T18085 T18084 arg1Of cells,T
R13653 T18088 T18086 arg1Of cells,CD4+
R13654 T18088 T18087 arg1Of cells,T
R13655 T18088 T18089 arg1Of cells,were
R13656 T18088 T18090 arg2Of cells,isolated
R13657 T18090 T18089 arg2Of isolated,were
R13658 T18090 T18091 arg1Of isolated,from
R13659 T18092 T18091 arg2Of blood,from
R13660 T18092 T18093 arg1Of blood,of
R13661 T18096 T18093 arg2Of volunteers,of
R13662 T18096 T18094 arg1Of volunteers,healthy
R13663 T18096 T18095 arg1Of volunteers,human
R13664 T18096 T18097 arg1Of volunteers,using
R13665 T18096 T18109 arg1Of volunteers,as
R13666 T18097 T18102 arg1Of using,(
R13667 T18101 T18097 arg2Of beads,using
R13668 T18101 T18098 arg1Of beads,the
R13669 T18101 T18099 arg1Of beads,anti-CD4
R13670 T18101 T18100 arg1Of beads,magnetic
R13671 T18103 T18104 arg1Of Dynal,","
R13672 T18104 T18102 arg2Of ",",(
R13673 T18104 T18106 arg1Of ",",","
R13674 T18105 T18104 arg2Of Hamburg,","
R13675 T18107 T18106 arg2Of Germany,","
R13676 T18108 T18102 arg3Of ),(
R13677 T18111 T18110 arg1Of described,previously
R13678 T18113 T18109 arg2Of 60,as
R13679 T18113 T18111 arg1Of 60,described
R13680 T18113 T18112 arg2Of 60,[
R13681 T18114 T18112 arg3Of ],[
R13682 T18116 T18115 arg1Of purity,The
R13683 T18116 T18117 arg1Of purity,of
R13684 T18116 T18121 arg1Of purity,was
R13685 T18116 T18123 arg2Of purity,tested
R13686 T18116 T18127 arg1Of purity,was
R13687 T18120 T18117 arg2Of cells,of
R13688 T18120 T18118 arg1Of cells,CD4+
R13689 T18120 T18119 arg1Of cells,T
R13690 T18123 T18121 arg2Of tested,was
R13691 T18123 T18122 arg1Of tested,initially
R13692 T18123 T18126 arg1Of tested,and
R13693 T18125 T18123 arg1Of FACS,tested
R13694 T18125 T18124 arg2Of FACS,by
R13695 T18127 T18126 arg2Of was,and
R13696 T18129 T18128 arg1Of 95,≥
R13697 T18130 T18127 arg2Of %,was
R13698 T18130 T18129 arg1Of %,95
R13699 T18131 T18132 arg1Of Monoclonality,of
R13700 T18131 T18136 arg1Of Monoclonality,was
R13701 T18131 T18137 arg2Of Monoclonality,confirmed
R13702 T18131 T18142 arg1Of Monoclonality,was
R13703 T18131 T18143 arg2Of Monoclonality,identified
R13704 T18131 T18145 arg1Of Monoclonality,be
R13705 T18131 T18147 arg1Of Monoclonality,positive
R13706 T18135 T18132 arg2Of clones,of
R13707 T18135 T18133 arg1Of clones,T
R13708 T18135 T18134 arg1Of clones,cell
R13709 T18137 T18136 arg2Of confirmed,was
R13710 T18137 T18141 arg1Of confirmed,and
R13711 T18140 T18137 arg1Of mapping,confirmed
R13712 T18140 T18138 arg2Of mapping,by
R13713 T18140 T18139 arg1Of mapping,TCR-chain
R13714 T18143 T18141 arg2Of identified,and
R13715 T18143 T18142 arg2Of identified,was
R13716 T18145 T18143 arg3Of be,identified
R13717 T18145 T18144 arg1Of be,to
R13718 T18147 T18145 arg2Of positive,be
R13719 T18147 T18146 arg1Of positive,Vbeta8
R13720 T18149 T18148 arg1Of clones,The
R13721 T18149 T18150 arg1Of clones,were
R13722 T18149 T18151 arg2Of clones,characterized
R13723 T18151 T18150 arg2Of characterized,were
R13724 T18155 T18151 arg1Of secretion,characterized
R13725 T18155 T18152 arg2Of secretion,by
R13726 T18155 T18153 arg1Of secretion,high
R13727 T18155 T18154 arg1Of secretion,IL-4
R14159 T18796 T18793 arg1Of culture,Inducible
R14160 T18796 T18794 arg1Of culture,murine
R14161 T18796 T18795 arg1Of culture,Treg
R14162 T18800 T18797 arg1Of cells,Naive
R14163 T18800 T18798 arg1Of cells,CD4+
R14164 T18800 T18799 arg1Of cells,T
R14165 T18800 T18801 arg1Of cells,(
R14166 T18800 T18809 arg1Of cells,were
R14167 T18800 T18810 arg2Of cells,isolated
R14168 T18802 T18803 arg1Of CD4+,","
R14169 T18803 T18806 arg1Of ",",and
R14170 T18804 T18803 arg2Of CD62L+,","
R14171 T18806 T18801 arg2Of and,(
R14172 T18806 T18805 arg1Of and,","
R14173 T18807 T18806 arg2Of CD25–,and
R14174 T18808 T18801 arg3Of ),(
R14175 T18810 T18809 arg2Of isolated,were
R14176 T18810 T18811 arg1Of isolated,from
R14177 T18814 T18812 arg2Of nodes,pooled
R14178 T18814 T18813 arg1Of nodes,lymph
R14179 T18814 T18815 arg1Of nodes,and
R14180 T18815 T18811 arg2Of and,from
R14181 T18815 T18817 arg1Of and,by
R14182 T18816 T18815 arg2Of spleens,and
R14183 T18818 T18817 arg2Of FACS,by
R14184 T18818 T18819 arg1Of FACS,(
R14185 T18821 T18819 arg2Of Aria,(
R14186 T18821 T18820 arg1Of Aria,FACS
R14187 T18821 T18822 arg1Of Aria,","
R14188 T18824 T18822 arg2Of Biosciences,","
R14189 T18824 T18823 arg1Of Biosciences,BD
R14190 T18825 T18819 arg3Of ),(
R14191 T18828 T18827 arg1Of 105,×
R14192 T18830 T18826 arg1Of cells,5
R14193 T18830 T18828 arg1Of cells,105
R14194 T18830 T18829 arg1Of cells,T
R14195 T18830 T18831 arg1Of cells,were
R14196 T18830 T18832 arg2Of cells,co-cultured
R14197 T18832 T18831 arg2Of co-cultured,were
R14198 T18832 T18833 arg1Of co-cultured,with
R14199 T18832 T18852 arg1Of co-cultured,in
R14200 T18837 T18835 arg1Of bone,×
R14201 T18837 T18836 arg1Of bone,104
R14202 T18838 T18837 arg1Of marrow–derived,bone
R14203 T18840 T18834 arg1Of cells,2.5
R14204 T18840 T18838 arg1Of cells,marrow–derived
R14205 T18840 T18839 arg1Of cells,dendritic
R14206 T18840 T18841 arg1Of cells,[
R14207 T18840 T18844 arg1Of cells,and
R14208 T18842 T18841 arg2Of 62,[
R14209 T18843 T18841 arg3Of ],[
R14210 T18844 T18833 arg2Of and,with
R14211 T18848 T18844 arg2Of peptide,and
R14212 T18848 T18845 arg1Of peptide,0.01
R14213 T18848 T18846 arg1Of peptide,μg/ml
R14214 T18848 T18847 arg1Of peptide,OVA323–339
R14215 T18848 T18849 arg1Of peptide,(
R14216 T18850 T18849 arg2Of Ansynth,(
R14217 T18851 T18849 arg3Of ),(
R14218 T18854 T18855 arg1Of presence,or
R14219 T18855 T18852 arg2Of or,in
R14220 T18855 T18853 arg1Of or,the
R14221 T18855 T18857 arg1Of or,of
R14222 T18856 T18855 arg2Of absence,or
R14223 T18859 T18857 arg2Of rhTGF-ß1,of
R14224 T18859 T18858 arg1Of rhTGF-ß1,20-ng/μl
R14225 T18859 T18860 arg1Of rhTGF-ß1,(
R14226 T18859 T18863 arg1Of rhTGF-ß1,in
R14227 T18861 T18860 arg2Of Peprotech,(
R14228 T18862 T18860 arg3Of ),(
R14229 T18865 T18863 arg2Of plates,in
R14230 T18865 T18864 arg1Of plates,48-well
R14231 T18868 T18866 arg2Of d,After
R14232 T18868 T18867 arg1Of d,4
R14233 T18870 T18871 arg1Of cells,were
R14234 T18870 T18872 arg2Of cells,harvested
R14235 T18870 T18874 arg2Of cells,analyzed
R14236 T18872 T18873 arg1Of harvested,and
R14237 T18873 T18866 arg1Of and,After
R14238 T18873 T18869 arg1Of and,","
R14239 T18873 T18871 arg2Of and,were
R14240 T18874 T18873 arg2Of analyzed,and
R14241 T18874 T18875 arg1Of analyzed,for
R14242 T18878 T18875 arg2Of expression,for
R14243 T18878 T18876 arg1Of expression,intracellular
R14244 T18878 T18877 arg1Of expression,FOXP3
R14245 T18878 T18879 arg1Of expression,by
R14246 T18878 T18884 arg1Of expression,by
R14247 T18880 T18881 arg1Of FACS,or
R14248 T18882 T18881 arg2Of gene,or
R14249 T18883 T18879 arg2Of expression,by
R14250 T18883 T18880 arg1Of expression,FACS
R14251 T18883 T18882 arg1Of expression,gene
R14252 T18886 T18884 arg2Of RT-PCR,by
R14253 T18886 T18885 arg1Of RT-PCR,quantitative
R14371 T19114 T19113 arg1Of vitro,In
R14372 T19117 T19114 arg1Of differentiation,vitro
R14373 T19117 T19115 arg1Of differentiation,T
R14374 T19117 T19116 arg1Of differentiation,cell
R14375 T19120 T19118 arg1Of magnetically-sorted,CD4+
R14376 T19120 T19119 arg1Of magnetically-sorted,CD45RA+
R14377 T19120 T19121 arg1Of magnetically-sorted,(
R14378 T19123 T19121 arg2Of depletion,(
R14379 T19123 T19122 arg1Of depletion,CD45RO
R14380 T19123 T19124 arg1Of depletion,","
R14381 T19123 T19126 arg1Of depletion,","
R14382 T19123 T19127 arg1Of depletion,according
R14383 T19125 T19124 arg2Of MACS,","
R14384 T19128 T19127 arg2Of to,according
R14385 T19130 T19128 arg2Of protocol,to
R14386 T19130 T19129 arg1Of protocol,the
R14387 T19130 T19131 arg1Of protocol,of
R14388 T19133 T19131 arg2Of manufacturer,of
R14389 T19133 T19132 arg1Of manufacturer,the
R14390 T19134 T19121 arg3Of ),(
R14391 T19135 T19120 arg1Of cells,magnetically-sorted
R14392 T19135 T19136 arg1Of cells,were
R14393 T19135 T19137 arg2Of cells,stimulated
R14394 T19137 T19136 arg2Of stimulated,were
R14395 T19137 T19138 arg1Of stimulated,with
R14396 T19137 T19159 arg1Of stimulated,:
R14397 T19141 T19139 arg2Of anti-CD3,immobilized
R14398 T19141 T19140 arg1Of anti-CD3,plate-bound
R14399 T19141 T19142 arg1Of anti-CD3,(
R14400 T19141 T19150 arg1Of anti-CD3,and
R14401 T19144 T19142 arg2Of μg/ml,(
R14402 T19144 T19143 arg1Of μg/ml,1
R14403 T19144 T19145 arg1Of μg/ml,","
R14404 T19144 T19147 arg1Of μg/ml,","
R14405 T19146 T19145 arg2Of Okt3,","
R14406 T19148 T19147 arg2Of IgG1,","
R14407 T19149 T19142 arg3Of ),(
R14408 T19150 T19138 arg2Of and,with
R14409 T19151 T19150 arg2Of anti-CD28,and
R14410 T19151 T19152 arg1Of anti-CD28,(
R14411 T19151 T19156 arg1Of anti-CD28,in
R14412 T19154 T19152 arg2Of μg/ml,(
R14413 T19154 T19153 arg1Of μg/ml,2
R14414 T19155 T19152 arg3Of ),(
R14415 T19158 T19156 arg2Of conditions,in
R14416 T19158 T19157 arg1Of conditions,Th1
R14417 T19162 T19160 arg1Of IL-12,25
R14418 T19162 T19161 arg1Of IL-12,ng/ml
R14419 T19162 T19163 arg1Of IL-12,","
R14420 T19163 T19177 arg1Of ",",:
R14421 T19166 T19164 arg1Of anti-IL-4,5
R14422 T19166 T19165 arg1Of anti-IL-4,μg/ml
R14423 T19166 T19167 arg1Of anti-IL-4,(
R14424 T19166 T19173 arg1Of anti-IL-4,;
R14425 T19171 T19167 arg2Of systems,(
R14426 T19171 T19168 arg1Of systems,R
R14427 T19171 T19169 arg1Of systems,&
R14428 T19171 T19170 arg1Of systems,D
R14429 T19172 T19167 arg3Of ),(
R14430 T19174 T19166 arg1Of in,anti-IL-4
R14431 T19176 T19163 arg2Of conditions,","
R14432 T19176 T19174 arg1Of conditions,in
R14433 T19176 T19175 arg1Of conditions,Th2
R14434 T19180 T19178 arg1Of IL-4,25
R14435 T19180 T19179 arg1Of IL-4,ng/ml
R14436 T19180 T19181 arg1Of IL-4,","
R14437 T19181 T19183 arg1Of ",",μg/ml
R14438 T19181 T19197 arg1Of ",",in
R14439 T19181 T19200 arg1Of ",",:
R14440 T19182 T19181 arg2Of 5,","
R14441 T19183 T19196 arg1Of μg/ml,or
R14442 T19184 T19183 arg2Of anti-IFN-γ,μg/ml
R14443 T19184 T19185 arg1Of anti-IFN-γ,","
R14444 T19188 T19185 arg2Of anti-IL-12,","
R14445 T19188 T19186 arg1Of anti-IL-12,5
R14446 T19188 T19187 arg1Of anti-IL-12,μg/ml
R14447 T19188 T19189 arg1Of anti-IL-12,(
R14448 T19193 T19189 arg2Of systems,(
R14449 T19193 T19190 arg1Of systems,R
R14450 T19193 T19191 arg1Of systems,&
R14451 T19193 T19192 arg1Of systems,D
R14452 T19194 T19189 arg3Of ),(
R14453 T19196 T19195 arg1Of or,;
R14454 T19197 T19196 arg2Of in,or
R14455 T19199 T19197 arg2Of conditions,in
R14456 T19199 T19198 arg1Of conditions,Treg
R14457 T19203 T19201 arg1Of TGF-β,10
R14458 T19203 T19202 arg1Of TGF-β,ng/ml
R14459 T19203 T19204 arg1Of TGF-β,","
R14460 T19203 T19212 arg1Of TGF-β,","
R14461 T19207 T19206 arg1Of anti-IFN-γ,μg/ml
R14462 T19207 T19208 arg1Of anti-IFN-γ,","
R14463 T19209 T19208 arg2Of 5,","
R14464 T19211 T19204 arg2Of anti-IL-12,","
R14465 T19211 T19205 arg1Of anti-IL-12,5
R14466 T19211 T19207 arg1Of anti-IL-12,anti-IFN-γ
R14467 T19211 T19209 arg1Of anti-IL-12,5
R14468 T19211 T19210 arg1Of anti-IL-12,μg/ml
R14469 T19215 T19212 arg2Of anti-IL-4,","
R14470 T19215 T19213 arg1Of anti-IL-4,5
R14471 T19215 T19214 arg1Of anti-IL-4,μg/ml
R14472 T19217 T19216 arg1Of cells,Proliferating
R14473 T19217 T19218 arg1Of cells,were
R14474 T19217 T19219 arg2Of cells,expanded
R14475 T19219 T19218 arg2Of expanded,were
R14476 T19219 T19220 arg1Of expanded,in
R14477 T19221 T19220 arg2Of medium,in
R14478 T19221 T19222 arg1Of medium,containing
R14479 T19223 T19222 arg2Of IL-2,containing
R14480 T19223 T19224 arg1Of IL-2,(
R14481 T19226 T19224 arg2Of ng/ml,(
R14482 T19226 T19225 arg1Of ng/ml,30
R14483 T19227 T19224 arg3Of ),(
R14636 T19534 T19536 arg2Of promoter,cloned
R14637 T19536 T19535 arg2Of cloned,was
R14638 T19536 T19537 arg1Of cloned,into
R14639 T19536 T19546 modOf cloned,to
R14640 T19541 T19537 arg2Of vector,into
R14641 T19541 T19538 arg1Of vector,the
R14642 T19541 T19539 arg1Of vector,pGL3
R14643 T19541 T19540 arg1Of vector,basic
R14644 T19541 T19542 arg1Of vector,(
R14645 T19544 T19542 arg2Of Biotech,(
R14646 T19544 T19543 arg1Of Biotech,Promega
R14647 T19545 T19542 arg3Of ),(
R14648 T19547 T19546 arg1Of generate,to
R14649 T19550 T19547 arg2Of FOXP3,generate
R14650 T19550 T19548 arg1Of FOXP3,the
R14651 T19550 T19549 arg1Of FOXP3,pGL3
R14652 T19550 T19551 arg1Of FOXP3,−511/+176
R14653 T19550 T19552 arg1Of FOXP3,[
R14654 T19553 T19552 arg2Of 24,[
R14655 T19554 T19552 arg3Of ],[
R14656 T19556 T19555 arg1Of mutagenesis,Site-directed
R14657 T19556 T19557 arg1Of mutagenesis,in
R14658 T19556 T19562 arg1Of mutagenesis,were
R14659 T19556 T19563 arg2Of mutagenesis,introduced
R14660 T19561 T19557 arg2Of region,in
R14661 T19561 T19558 arg1Of region,the
R14662 T19561 T19559 arg1Of region,FOXP3
R14663 T19561 T19560 arg1Of region,promoter
R14664 T19563 T19562 arg2Of introduced,were
R14665 T19563 T19564 modOf introduced,using
R14666 T19563 T19571 arg1Of introduced,","
R14667 T19563 T19572 arg1Of introduced,according
R14668 T19567 T19564 arg2Of kit,using
R14669 T19567 T19565 arg1Of kit,the
R14670 T19567 T19566 arg1Of kit,QuickChange
R14671 T19567 T19568 arg1Of kit,(
R14672 T19569 T19568 arg2Of Stratagene,(
R14673 T19570 T19568 arg3Of ),(
R14674 T19573 T19572 arg2Of to,according
R14675 T19575 T19574 arg1Of manufacturer,the
R14676 T19575 T19576 arg2Of manufacturer,'s
R14677 T19577 T19573 arg2Of instructions,to
R14678 T19577 T19576 arg1Of instructions,'s
R14679 T19580 T19578 arg1Of primer,The
R14680 T19580 T19579 arg1Of primer,following
R14681 T19580 T19581 arg1Of primer,and
R14682 T19581 T19585 arg1Of and,were
R14683 T19581 T19586 arg2Of and,used
R14684 T19584 T19581 arg2Of strand,and
R14685 T19584 T19582 arg1Of strand,its
R14686 T19584 T19583 arg1Of strand,complementary
R14687 T19586 T19585 arg2Of used,were
R14688 T19586 T19587 arg1Of used,:
R14689 T19592 T19588 arg1Of CCC,GTT
R14690 T19592 T19589 arg1Of CCC,TCT
R14691 T19592 T19590 arg1Of CCC,CAT
R14692 T19592 T19591 arg1Of CCC,GAG
R14693 T19598 T19592 arg1Of ACC,CCC
R14694 T19598 T19593 arg1Of ACC,TAT
R14695 T19598 T19594 arg1Of ACC,TAA
R14696 T19598 T19595 arg1Of ACC,GTC
R14697 T19598 T19596 arg1Of ACC,ATT
R14698 T19598 T19597 arg1Of ACC,CTT
R14699 T19604 T19586 arg3Of GA,used
R14700 T19604 T19598 arg1Of GA,ACC
R14701 T19604 T19599 arg1Of GA,TCT
R14702 T19604 T19600 arg1Of GA,CAC
R14703 T19604 T19601 arg1Of GA,CTC
R14704 T19604 T19602 arg1Of GA,TGT
R14705 T19604 T19603 arg1Of GA,GGT
R14795 T19770 T19771 arg1Of Transfections,and
R14796 T19774 T19771 arg2Of assays,and
R14797 T19774 T19772 arg1Of assays,reporter
R14798 T19774 T19773 arg1Of assays,gene
R14799 T19776 T19775 arg1Of cells,T
R14800 T19776 T19777 arg1Of cells,were
R14801 T19776 T19778 arg2Of cells,rested
R14802 T19778 T19777 arg2Of rested,were
R14803 T19778 T19779 arg1Of rested,in
R14804 T19778 T19787 arg1Of rested,overnight
R14805 T19782 T19779 arg2Of medium,in
R14806 T19782 T19780 arg1Of medium,serum-free
R14807 T19782 T19781 arg1Of medium,AIM-V
R14808 T19782 T19783 arg1Of medium,(
R14809 T19785 T19783 arg2Of Technologies,(
R14810 T19785 T19784 arg1Of Technologies,Life
R14811 T19786 T19783 arg3Of ),(
R14812 T19789 T19788 arg1Of μg,3.5
R14813 T19789 T19790 arg1Of μg,of
R14814 T19789 T19797 arg1Of μg,and
R14815 T19796 T19790 arg2Of vector,of
R14816 T19796 T19791 arg1Of vector,the
R14817 T19796 T19792 arg1Of vector,FOXP3
R14818 T19796 T19793 arg1Of vector,promoter
R14819 T19796 T19794 arg1Of vector,luciferase
R14820 T19796 T19795 arg1Of vector,reporter
R14821 T19797 T19801 arg1Of and,were
R14822 T19797 T19802 arg2Of and,added
R14823 T19797 T19822 arg2Of and,electroporated
R14824 T19797 T19823 arg1Of and,using
R14825 T19800 T19797 arg2Of phRL-TK,and
R14826 T19800 T19798 arg1Of phRL-TK,0.5
R14827 T19800 T19799 arg1Of phRL-TK,μg
R14828 T19802 T19803 arg1Of added,to
R14829 T19802 T19821 arg1Of added,and
R14830 T19806 T19805 arg1Of 106,×
R14831 T19809 T19803 arg2Of cells,to
R14832 T19809 T19804 arg1Of cells,3
R14833 T19809 T19806 arg1Of cells,106
R14834 T19809 T19807 arg1Of cells,CD4+
R14835 T19809 T19808 arg1Of cells,T
R14836 T19809 T19810 arg2Of cells,resuspended
R14837 T19810 T19811 arg1Of resuspended,in
R14838 T19813 T19811 arg2Of μL,in
R14839 T19813 T19812 arg1Of μL,100
R14840 T19813 T19814 arg1Of μL,of
R14841 T19816 T19814 arg2Of solution,of
R14842 T19816 T19815 arg1Of solution,Nucleofector
R14843 T19816 T19817 arg1Of solution,(
R14844 T19819 T19817 arg2Of Biosystems,(
R14845 T19819 T19818 arg1Of Biosystems,Amaxa
R14846 T19820 T19817 arg3Of ),(
R14847 T19821 T19801 arg2Of and,were
R14848 T19822 T19821 arg2Of electroporated,and
R14849 T19823 T19822 arg3Of using,electroporated
R14850 T19823 T19830 arg1Of using,After
R14851 T19823 T19834 arg1Of using,in
R14852 T19826 T19823 arg2Of program,using
R14853 T19826 T19824 arg1Of program,the
R14854 T19826 T19825 arg1Of program,U-15
R14855 T19826 T19827 arg1Of program,of
R14856 T19829 T19827 arg2Of Nucleofector.,of
R14857 T19829 T19828 arg1Of Nucleofector.,the
R14858 T19833 T19830 arg2Of culture,After
R14859 T19833 T19831 arg1Of culture,a
R14860 T19833 T19832 arg1Of culture,24-h
R14861 T19836 T19837 arg1Of conditions,and
R14862 T19837 T19834 arg2Of and,in
R14863 T19837 T19835 arg1Of and,serum-free
R14864 T19837 T19839 arg1Of and,as
R14865 T19837 T19847 modOf and,was
R14866 T19838 T19837 arg2Of stimuli,and
R14867 T19840 T19839 arg2Of indicated,as
R14868 T19840 T19841 arg1Of indicated,in
R14869 T19843 T19841 arg2Of figures,in
R14870 T19843 T19842 arg1Of figures,the
R14871 T19846 T19845 arg1Of activity,luciferase
R14872 T19846 T19847 arg1Of activity,was
R14873 T19846 T19848 arg2Of activity,measured
R14874 T19848 T19844 arg1Of measured,","
R14875 T19848 T19847 arg2Of measured,was
R14876 T19848 T19849 arg1Of measured,by
R14877 T19848 T19859 arg1Of measured,according
R14878 T19854 T19849 arg2Of system,by
R14879 T19854 T19850 arg1Of system,the
R14880 T19854 T19851 arg1Of system,dual
R14881 T19854 T19852 arg1Of system,luciferase
R14882 T19854 T19853 arg1Of system,assay
R14883 T19854 T19855 arg1Of system,(
R14884 T19857 T19855 arg2Of Biotech,(
R14885 T19857 T19856 arg1Of Biotech,Promega
R14886 T19858 T19855 arg3Of ),(
R14887 T19860 T19859 arg2Of to,according
R14888 T19862 T19861 arg1Of manufacturer,the
R14889 T19862 T19863 arg2Of manufacturer,'s
R14890 T19864 T19860 arg2Of instructions,to
R14891 T19864 T19863 arg1Of instructions,'s
R14892 T19865 T19866 arg1Of Data,were
R14893 T19865 T19867 arg2Of Data,normalized
R14894 T19867 T19866 arg2Of normalized,were
R14895 T19870 T19867 arg1Of activity,normalized
R14896 T19870 T19868 arg2Of activity,by
R14897 T19870 T19869 arg1Of activity,the
R14898 T19870 T19871 arg1Of activity,of
R14899 T19873 T19871 arg2Of luciferase,of
R14900 T19873 T19872 arg1Of luciferase,renilla
R15017 T20058 T20057 arg2Of isolated,was
R15018 T20059 T20058 arg3Of using,isolated
R15019 T20059 T20067 arg1Of using,according
R15020 T20063 T20059 arg2Of Kit,using
R15021 T20063 T20060 arg1Of Kit,the
R15022 T20063 T20061 arg1Of Kit,RNeasy
R15023 T20063 T20062 arg1Of Kit,Mini
R15024 T20063 T20064 arg1Of Kit,(
R15025 T20065 T20064 arg2Of Qiagen,(
R15026 T20066 T20064 arg3Of ),(
R15027 T20068 T20067 arg2Of to,according
R15028 T20070 T20069 arg1Of manufacturer,the
R15029 T20070 T20071 arg2Of manufacturer,'s
R15030 T20072 T20068 arg2Of protocol,to
R15031 T20072 T20071 arg1Of protocol,'s
R15032 T20074 T20073 arg1Of transcription,Reverse
R15033 T20074 T20075 arg1Of transcription,of
R15034 T20074 T20078 arg1Of transcription,was
R15035 T20074 T20079 arg2Of transcription,performed
R15036 T20077 T20075 arg2Of samples,of
R15037 T20077 T20076 arg1Of samples,human
R15038 T20079 T20078 arg2Of performed,was
R15039 T20079 T20080 arg1Of performed,with
R15040 T20079 T20091 arg1Of performed,according
R15041 T20083 T20080 arg2Of reagents,with
R15042 T20083 T20081 arg1Of reagents,TaqMan
R15043 T20083 T20082 arg1Of reagents,reverse-transcription
R15044 T20083 T20084 arg1Of reagents,(
R15045 T20083 T20088 arg1Of reagents,with
R15046 T20086 T20084 arg2Of Biosystems,(
R15047 T20086 T20085 arg1Of Biosystems,Applied
R15048 T20087 T20084 arg3Of ),(
R15049 T20090 T20088 arg2Of hexamers,with
R15050 T20090 T20089 arg1Of hexamers,random
R15051 T20092 T20091 arg2Of to,according
R15052 T20094 T20093 arg1Of manufacturer,the
R15053 T20094 T20095 arg2Of manufacturer,'s
R15054 T20096 T20092 arg2Of protocol,to
R15055 T20096 T20095 arg1Of protocol,'s
R15111 T20177 T20175 arg1Of proteins,Recombinant
R15112 T20177 T20176 arg1Of proteins,TAT
R15113 T20179 T20178 arg1Of cDNAs,The
R15114 T20179 T20180 arg1Of cDNAs,encoding
R15115 T20179 T20187 arg1Of cDNAs,(
R15116 T20179 T20194 arg1Of cDNAs,were
R15117 T20179 T20195 arg2Of cDNAs,cloned
R15118 T20182 T20181 arg1Of protein,GATA3
R15119 T20182 T20183 arg1Of protein,or
R15120 T20183 T20180 arg2Of or,encoding
R15121 T20186 T20183 arg2Of GATA3,or
R15122 T20186 T20184 arg1Of GATA3,the
R15123 T20186 T20185 arg2Of GATA3,truncated
R15124 T20188 T20187 arg2Of lacking,(
R15125 T20192 T20188 arg2Of fingers,lacking
R15126 T20192 T20189 arg1Of fingers,the
R15127 T20192 T20190 arg1Of fingers,two
R15128 T20192 T20191 arg1Of fingers,zinc
R15129 T20193 T20187 arg3Of ),(
R15130 T20195 T20194 arg2Of cloned,were
R15131 T20195 T20196 arg1Of cloned,in
R15132 T20195 T20198 arg1Of cloned,into
R15133 T20195 T20203 arg1Of cloned,with
R15134 T20197 T20196 arg2Of frame,in
R15135 T20201 T20198 arg2Of vector,into
R15136 T20201 T20199 arg1Of vector,an
R15137 T20201 T20200 arg1Of vector,expression
R15138 T20203 T20202 arg1Of with,along
R15139 T20206 T20203 arg2Of sequence,with
R15140 T20206 T20204 arg1Of sequence,the
R15141 T20206 T20205 arg1Of sequence,TAT
R15142 T20206 T20207 arg1Of sequence,as
R15143 T20209 T20208 arg1Of described,previously
R15144 T20211 T20207 arg2Of 63,as
R15145 T20211 T20209 arg1Of 63,described
R15146 T20211 T20210 arg2Of 63,[
R15147 T20212 T20210 arg3Of ],[
R15148 T20213 T20214 arg1Of Proteins,were
R15149 T20213 T20215 arg2Of Proteins,expressed
R15150 T20215 T20214 arg2Of expressed,were
R15151 T20215 T20216 arg1Of expressed,in
R15152 T20215 T20226 arg1Of expressed,and
R15153 T20218 T20217 arg1Of Star,BL21
R15154 T20218 T20219 arg1Of Star,(
R15155 T20220 T20219 arg2Of DE3,(
R15156 T20221 T20219 arg3Of ),(
R15157 T20222 T20216 arg2Of pLysS,in
R15158 T20222 T20218 arg1Of pLysS,Star
R15159 T20222 T20223 arg1Of pLysS,(
R15160 T20224 T20223 arg2Of Invitrogen,(
R15161 T20225 T20223 arg3Of ),(
R15162 T20227 T20228 arg1Of lysates,were
R15163 T20227 T20229 arg2Of lysates,purified
R15164 T20229 T20226 arg2Of purified,and
R15165 T20229 T20228 arg2Of purified,were
R15166 T20233 T20229 arg1Of chromatography,purified
R15167 T20233 T20230 arg2Of chromatography,by
R15168 T20233 T20231 arg1Of chromatography,Ni2+-chelate
R15169 T20233 T20232 arg1Of chromatography,column
R15170 T20236 T20234 arg1Of proteins,Both
R15171 T20236 T20235 arg1Of proteins,TAT-linked
R15172 T20236 T20237 arg1Of proteins,were
R15173 T20237 T20243 arg1Of were,","
R15174 T20237 T20244 arg1Of were,based
R15175 T20238 T20239 arg1Of more,than
R15176 T20240 T20239 arg2Of 95,than
R15177 T20240 T20241 arg1Of 95,%
R15178 T20242 T20237 arg2Of pure,were
R15179 T20242 T20238 arg1Of pure,more
R15180 T20245 T20244 arg2Of on,based
R15181 T20248 T20245 arg2Of staining,on
R15182 T20248 T20246 arg1Of staining,Coomassie
R15183 T20248 T20247 arg1Of staining,blue
R15184 T20248 T20249 arg1Of staining,of
R15185 T20253 T20249 arg2Of gels,of
R15186 T20253 T20250 arg1Of gels,sodium
R15187 T20253 T20251 arg1Of gels,disulfate
R15188 T20253 T20252 arg1Of gels,acrylamide
R15291 T20446 T20445 arg1Of transduction,TAT-GATA3
R15292 T20448 T20447 arg1Of cells,CD4+CD45RA+
R15293 T20448 T20449 arg1Of cells,were
R15294 T20448 T20450 arg2Of cells,cultured
R15295 T20448 T20455 arg2Of cells,transduced
R15296 T20450 T20451 arg1Of cultured,in
R15297 T20450 T20454 arg1Of cultured,and
R15298 T20453 T20451 arg2Of medium,in
R15299 T20453 T20452 arg1Of medium,AIMV
R15300 T20454 T20449 arg2Of and,were
R15301 T20455 T20454 arg2Of transduced,and
R15302 T20455 T20456 arg1Of transduced,with
R15303 T20455 T20471 arg1Of transduced,over
R15304 T20458 T20457 arg1Of nM,20
R15305 T20458 T20459 arg1Of nM,","
R15306 T20459 T20463 arg1Of ",",or
R15307 T20461 T20459 arg2Of nM,","
R15308 T20461 T20460 arg1Of nM,10
R15309 T20463 T20456 arg2Of or,with
R15310 T20463 T20462 arg1Of or,","
R15311 T20465 T20463 arg2Of nM,or
R15312 T20465 T20464 arg1Of nM,500
R15313 T20465 T20466 arg1Of nM,of
R15314 T20467 T20468 arg1Of full-length,or
R15315 T20469 T20468 arg2Of truncated,or
R15316 T20470 T20466 arg2Of GATA3,of
R15317 T20470 T20467 arg1Of GATA3,full-length
R15318 T20470 T20469 arg2Of GATA3,truncated
R15319 T20473 T20471 arg2Of course,over
R15320 T20473 T20472 arg1Of course,the
R15321 T20473 T20474 arg1Of course,of
R15322 T20476 T20474 arg2Of h,of
R15323 T20476 T20475 arg1Of h,4
R15324 T20479 T20477 arg2Of h,After
R15325 T20479 T20478 arg1Of h,4
R15326 T20482 T20481 arg1Of cells,the
R15327 T20482 T20483 arg1Of cells,were
R15328 T20482 T20484 arg2Of cells,washed
R15329 T20482 T20486 arg2Of cells,activated
R15330 T20484 T20485 arg1Of washed,and
R15331 T20485 T20477 arg1Of and,After
R15332 T20485 T20480 arg1Of and,","
R15333 T20485 T20483 arg2Of and,were
R15334 T20486 T20485 arg2Of activated,and
R15335 T20486 T20487 arg1Of activated,with
R15336 T20489 T20490 arg1Of anti-CD3,and
R15337 T20490 T20488 arg1Of and,soluble
R15338 T20490 T20492 arg1Of and,and
R15339 T20491 T20490 arg2Of anti-CD28,and
R15340 T20492 T20487 arg2Of and,with
R15341 T20493 T20492 arg2Of TGF-β,and
R15342 T20493 T20494 arg1Of TGF-β,(
R15343 T20496 T20494 arg2Of ng/ml,(
R15344 T20496 T20495 arg1Of ng/ml,10
R15345 T20497 T20494 arg3Of ),(
R15346 T20499 T20498 arg1Of day,Each
R15347 T20503 T20501 arg1Of proteins,the
R15348 T20503 T20502 arg1Of proteins,TAT
R15349 T20503 T20504 arg1Of proteins,were
R15350 T20503 T20506 arg2Of proteins,added
R15351 T20506 T20499 arg1Of added,day
R15352 T20506 T20500 arg1Of added,","
R15353 T20506 T20504 arg2Of added,were
R15354 T20506 T20505 arg1Of added,freshly
R15355 T20506 T20507 arg1Of added,to
R15356 T20509 T20507 arg2Of medium,to
R15357 T20509 T20508 arg1Of medium,the
R15358 T20511 T20510 arg1Of expression,FOXP3
R15359 T20511 T20512 arg1Of expression,was
R15360 T20511 T20513 arg2Of expression,measured
R15361 T20513 T20512 arg2Of measured,was
R15362 T20513 T20514 arg1Of measured,after
R15363 T20516 T20514 arg2Of d,after
R15364 T20516 T20515 arg1Of d,5
R15365 T20516 T20517 arg1Of d,by
R15366 T20519 T20517 arg2Of staining,by
R15367 T20519 T20518 arg1Of staining,intracellular
R15458 T20717 T20715 arg1Of staining,Intracellular
R15459 T20717 T20716 arg1Of staining,cytokine
R15460 T20719 T20718 arg1Of cells,T
R15461 T20719 T20720 arg1Of cells,were
R15462 T20719 T20721 arg2Of cells,stimulated
R15463 T20721 T20720 arg2Of stimulated,were
R15464 T20721 T20722 arg1Of stimulated,with
R15465 T20725 T20723 arg1Of 10−7,2
R15466 T20725 T20724 arg1Of 10−7,×
R15467 T20725 T20726 arg1Of 10−7,M
R15468 T20727 T20725 arg1Of PMA,10−7
R15469 T20727 T20728 arg1Of PMA,and
R15470 T20728 T20722 arg2Of and,with
R15471 T20730 T20728 arg2Of μg/ml,and
R15472 T20730 T20729 arg1Of μg/ml,1
R15473 T20730 T20731 arg1Of μg/ml,of
R15474 T20732 T20731 arg2Of ionomycin,of
R15475 T20732 T20733 arg1Of ionomycin,(
R15476 T20732 T20737 arg1Of ionomycin,for
R15477 T20735 T20733 arg2Of Chemicals,(
R15478 T20735 T20734 arg1Of Chemicals,Sigma
R15479 T20736 T20733 arg3Of ),(
R15480 T20739 T20737 arg2Of h,for
R15481 T20739 T20738 arg1Of h,4
R15482 T20742 T20740 arg1Of mAb,The
R15483 T20742 T20741 arg1Of mAb,following
R15484 T20742 T20743 arg1Of mAb,was
R15485 T20742 T20744 arg2Of mAb,used
R15486 T20744 T20743 arg2Of used,was
R15487 T20744 T20745 arg1Of used,:
R15488 T20744 T20746 arg1Of used,anti-IL-4-PE
R15489 T20746 T20747 arg1Of anti-IL-4-PE,(
R15490 T20748 T20747 arg2Of 8D4–8,(
R15491 T20748 T20749 arg1Of 8D4–8,","
R15492 T20750 T20749 arg2Of BD,","
R15493 T20751 T20747 arg3Of ),(
R15494 T20754 T20752 arg2Of controls,Matched
R15495 T20754 T20753 arg1Of controls,isotype
R15496 T20754 T20755 arg1Of controls,were
R15497 T20754 T20756 arg2Of controls,used
R15498 T20756 T20755 arg2Of used,were
R15499 T20756 T20757 arg1Of used,at
R15500 T20756 T20762 arg1Of used,as
R15501 T20761 T20757 arg2Of concentration,at
R15502 T20761 T20758 arg1Of concentration,the
R15503 T20761 T20759 arg1Of concentration,same
R15504 T20761 T20760 arg1Of concentration,protein
R15505 T20765 T20762 arg2Of antibodies,as
R15506 T20765 T20763 arg1Of antibodies,the
R15507 T20765 T20764 arg1Of antibodies,respective
R15508 T20767 T20766 arg1Of FACS,Four-color
R15509 T20767 T20768 arg1Of FACS,was
R15510 T20767 T20769 arg2Of FACS,performed
R15511 T20769 T20768 arg2Of performed,was
R15512 T20770 T20769 arg3Of using,performed
R15513 T20773 T20770 arg2Of XL-MCL,using
R15514 T20773 T20771 arg1Of XL-MCL,an
R15515 T20773 T20772 arg1Of XL-MCL,EPICS
R15516 T20773 T20774 arg1Of XL-MCL,(
R15517 T20773 T20778 arg1Of XL-MCL,using
R15518 T20776 T20774 arg2Of Coulter,(
R15519 T20776 T20775 arg1Of Coulter,Beckman
R15520 T20777 T20774 arg3Of ),(
R15521 T20782 T20778 arg2Of version,using
R15522 T20782 T20779 arg1Of version,the
R15523 T20782 T20780 arg1Of version,software
R15524 T20782 T20781 arg1Of version,Expo32
R15525 T20782 T20783 arg1Of version,for
R15526 T20785 T20786 arg1Of acquisition,and
R15527 T20786 T20783 arg2Of and,for
R15528 T20786 T20784 arg1Of and,data
R15529 T20787 T20786 arg2Of evaluation,and
R15619 T20969 T20968 arg1Of cytometry,Flow
R15620 T20971 T20970 arg2Of analysis,For
R15621 T20971 T20972 arg1Of analysis,of
R15622 T20974 T20972 arg2Of expression,of
R15623 T20974 T20973 arg1Of expression,FOXP3
R15624 T20974 T20975 arg1Of expression,at
R15625 T20978 T20975 arg2Of level,at
R15626 T20978 T20976 arg1Of level,the
R15627 T20978 T20977 arg1Of level,single-cell
R15628 T20980 T20981 arg1Of cells,were
R15629 T20980 T20983 arg2Of cells,stained
R15630 T20983 T20981 arg2Of stained,were
R15631 T20983 T20982 arg1Of stained,first
R15632 T20983 T20984 arg1Of stained,with
R15633 T20983 T20994 arg1Of stained,and
R15634 T20988 T20984 arg2Of CD25,with
R15635 T20988 T20985 arg1Of CD25,the
R15636 T20988 T20986 arg1Of CD25,monoclonal
R15637 T20988 T20987 arg1Of CD25,antibody
R15638 T20988 T20989 arg1Of CD25,(
R15639 T20991 T20989 arg2Of Coulter,(
R15640 T20991 T20990 arg1Of Coulter,Beckman
R15641 T20992 T20989 arg3Of ),(
R15642 T20994 T20970 arg1Of and,For
R15643 T20994 T20979 arg1Of and,","
R15644 T20994 T20993 arg1Of and,","
R15645 T20996 T20997 arg1Of fixation,and
R15646 T20997 T20995 arg2Of and,after
R15647 T20998 T20997 arg2Of permeabilization,and
R15648 T21000 T21001 arg1Of cells,were
R15649 T21000 T21002 arg2Of cells,incubated
R15650 T21000 T21021 arg2Of cells,subjected
R15651 T21002 T21003 arg1Of incubated,with
R15652 T21002 T21014 arg1Of incubated,based
R15653 T21002 T21020 arg1Of incubated,and
R15654 T21007 T21003 arg2Of PCH101,with
R15655 T21007 T21004 arg1Of PCH101,PE-conjugated
R15656 T21007 T21005 arg1Of PCH101,monoclonal
R15657 T21007 T21006 arg1Of PCH101,antibody
R15658 T21007 T21008 arg1Of PCH101,(
R15659 T21010 T21008 arg2Of FOXP3,(
R15660 T21010 T21009 arg1Of FOXP3,anti-human
R15661 T21010 T21011 arg1Of FOXP3,;
R15662 T21012 T21011 arg2Of eBioscience,;
R15663 T21013 T21008 arg3Of ),(
R15664 T21015 T21014 arg2Of on,based
R15665 T21017 T21016 arg1Of manufacturer,the
R15666 T21017 T21018 arg2Of manufacturer,'s
R15667 T21019 T21015 arg2Of recommendations,on
R15668 T21019 T21018 arg1Of recommendations,'s
R15669 T21020 T20994 arg2Of and,and
R15670 T21020 T20995 arg1Of and,after
R15671 T21020 T20999 arg1Of and,","
R15672 T21020 T21001 arg2Of and,were
R15673 T21021 T21020 arg2Of subjected,and
R15674 T21021 T21022 arg1Of subjected,to
R15675 T21023 T21022 arg2Of FACS,to
R15676 T21023 T21024 arg1Of FACS,(
R15677 T21026 T21024 arg2Of XL-MCL,(
R15678 T21026 T21025 arg1Of XL-MCL,EPICS
R15679 T21027 T21024 arg3Of ),(
R15680 T21032 T21028 arg2Of staining,For
R15681 T21032 T21029 arg1Of staining,cell
R15682 T21032 T21030 arg1Of staining,surface
R15683 T21032 T21031 arg1Of staining,marker
R15684 T21034 T21035 arg1Of cells,were
R15685 T21034 T21036 arg2Of cells,incubated
R15686 T21036 T21028 arg1Of incubated,For
R15687 T21036 T21033 arg1Of incubated,","
R15688 T21036 T21035 arg2Of incubated,were
R15689 T21036 T21037 arg1Of incubated,for
R15690 T21036 T21040 arg1Of incubated,at
R15691 T21036 T21045 arg1Of incubated,with
R15692 T21039 T21037 arg2Of min,for
R15693 T21039 T21038 arg1Of min,20
R15694 T21041 T21040 arg2Of 4°C,at
R15695 T21041 T21042 arg1Of 4°C,in
R15696 T21044 T21042 arg2Of buffer,in
R15697 T21044 T21043 arg1Of buffer,staining
R15698 T21048 T21045 arg2Of antibodies,with
R15699 T21048 T21046 arg1Of antibodies,the
R15700 T21048 T21047 arg1Of antibodies,following
R15701 T21048 T21049 arg1Of antibodies,:
R15702 T21050 T21051 arg1Of anti–CD152-PE,(
R15703 T21050 T21056 arg1Of anti–CD152-PE,","
R15704 T21052 T21051 arg2Of CTLA-4,(
R15705 T21052 T21053 arg1Of CTLA-4,;
R15706 T21054 T21053 arg2Of BD,;
R15707 T21055 T21051 arg3Of ),(
R15708 T21056 T21049 arg2Of ",",:
R15709 T21056 T21061 arg1Of ",",","
R15710 T21056 T21069 arg1Of ",",","
R15711 T21057 T21056 arg2Of anti–PD-1,","
R15712 T21057 T21058 arg1Of anti–PD-1,(
R15713 T21059 T21058 arg2Of eBiosciences,(
R15714 T21060 T21058 arg3Of ),(
R15715 T21062 T21061 arg2Of anti-GITR,","
R15716 T21062 T21063 arg1Of anti-GITR,(
R15717 T21067 T21063 arg2Of Systems,(
R15718 T21067 T21064 arg1Of Systems,R
R15719 T21067 T21065 arg1Of Systems,&
R15720 T21067 T21066 arg1Of Systems,D
R15721 T21068 T21063 arg3Of ),(
R15722 T21070 T21071 arg1Of anti-CD69,(
R15723 T21070 T21075 arg1Of anti-CD69,","
R15724 T21073 T21071 arg2Of Coulter,(
R15725 T21073 T21072 arg1Of Coulter,Beckman
R15726 T21074 T21071 arg3Of ),(
R15727 T21075 T21080 arg1Of ",",","
R15728 T21076 T21075 arg2Of anti-CD103,","
R15729 T21076 T21077 arg1Of anti-CD103,(
R15730 T21078 T21077 arg2Of DakoCytomation,(
R15731 T21079 T21077 arg3Of ),(
R15732 T21080 T21087 arg1Of ",",or
R15733 T21081 T21080 arg2Of anti-CD62L,","
R15734 T21081 T21082 arg1Of anti-CD62L,(
R15735 T21084 T21082 arg2Of Coulter,(
R15736 T21084 T21083 arg1Of Coulter,Beckman
R15737 T21085 T21082 arg3Of ),(
R15738 T21087 T21069 arg2Of or,","
R15739 T21087 T21086 arg1Of or,","
R15740 T21088 T21087 arg2Of anti-HLA-DR,or
R15741 T21088 T21089 arg1Of anti-HLA-DR,(
R15742 T21091 T21089 arg2Of Coulter,(
R15743 T21091 T21090 arg1Of Coulter,Beckman
R15744 T21092 T21089 arg3Of ),(
R15745 T21094 T21093 arg1Of controls,The
R15746 T21094 T21095 arg1Of controls,were
R15747 T21096 T21097 arg1Of FITC,","
R15748 T21097 T21100 arg1Of ",",or
R15749 T21098 T21097 arg2Of PE,","
R15750 T21100 T21095 arg2Of or,were
R15751 T21100 T21099 arg1Of or,","
R15752 T21103 T21101 arg1Of IgG1,ECD-conjugated
R15753 T21103 T21102 arg1Of IgG1,mouse
R15754 T21103 T21104 arg1Of IgG1,or
R15755 T21104 T21100 arg2Of or,or
R15756 T21106 T21104 arg2Of IgG2a,or
R15757 T21106 T21105 arg1Of IgG2a,rat
R15758 T21108 T21107 arg2Of staining,For
R15759 T21108 T21109 arg1Of staining,of
R15760 T21111 T21109 arg2Of cells,of
R15761 T21111 T21110 arg1Of cells,mouse
R15762 T21115 T21113 arg1Of mAbs,the
R15763 T21115 T21114 arg1Of mAbs,following
R15764 T21115 T21116 arg1Of mAbs,from
R15765 T21115 T21119 arg1Of mAbs,were
R15766 T21115 T21120 arg2Of mAbs,used
R15767 T21118 T21116 arg2Of Biosciences,from
R15768 T21118 T21117 arg1Of Biosciences,BD
R15769 T21120 T21107 arg1Of used,For
R15770 T21120 T21112 arg1Of used,","
R15771 T21120 T21119 arg2Of used,were
R15772 T21120 T21121 arg1Of used,following
R15773 T21120 T21124 arg1Of used,as
R15774 T21123 T21121 arg2Of techniques,following
R15775 T21123 T21122 arg1Of techniques,standard
R15776 T21125 T21124 arg2Of described,as
R15777 T21125 T21126 arg1Of described,above
R15778 T21126 T21127 arg1Of above,:
R15779 T21128 T21129 arg1Of anti-CD3,","
R15780 T21129 T21132 arg1Of ",",and
R15781 T21130 T21129 arg2Of anti-CD4,","
R15782 T21132 T21126 arg2Of and,above
R15783 T21132 T21131 arg1Of and,","
R15784 T21133 T21132 arg2Of anti-CD25,and
R15785 T21135 T21134 arg1Of antibody,Anti-FcγRII/III
R15786 T21135 T21136 arg1Of antibody,(
R15787 T21135 T21141 arg1Of antibody,was
R15788 T21135 T21142 arg2Of antibody,included
R15789 T21135 T21147 arg1Of antibody,reduce
R15790 T21137 T21136 arg2Of 2.4G2,(
R15791 T21137 T21138 arg1Of 2.4G2,","
R15792 T21139 T21138 arg2Of ATCC,","
R15793 T21140 T21136 arg3Of ),(
R15794 T21142 T21141 arg2Of included,was
R15795 T21142 T21143 arg1Of included,in
R15796 T21142 T21146 modOf included,to
R15797 T21145 T21143 arg2Of stainings,in
R15798 T21145 T21144 arg1Of stainings,all
R15799 T21147 T21146 arg1Of reduce,to
R15800 T21150 T21147 arg2Of binding,reduce
R15801 T21150 T21148 arg1Of binding,nonspecific
R15802 T21150 T21149 arg1Of binding,antibody
R15803 T21152 T21151 arg1Of isolate,To
R15804 T21157 T21152 arg2Of cells,isolate
R15805 T21157 T21153 arg1Of cells,naive
R15806 T21157 T21154 arg1Of cells,murine
R15807 T21157 T21155 arg1Of cells,CD4
R15808 T21157 T21156 arg1Of cells,T
R15809 T21157 T21158 arg1Of cells,from
R15810 T21160 T21159 arg1Of DO11.10,murine
R15811 T21160 T21161 arg1Of DO11.10,or
R15812 T21161 T21158 arg2Of or,from
R15813 T21164 T21161 arg2Of cells,or
R15814 T21164 T21162 arg1Of cells,DO11.10xCD2-GATA3
R15815 T21164 T21163 arg1Of cells,T
R15816 T21166 T21152 arg1Of cells,isolate
R15817 T21166 T21167 arg1Of cells,were
R15818 T21166 T21168 arg2Of cells,stained
R15819 T21168 T21151 modOf stained,To
R15820 T21168 T21165 arg1Of stained,","
R15821 T21168 T21167 arg2Of stained,were
R15822 T21168 T21169 arg1Of stained,with
R15823 T21170 T21171 arg1Of anti-CD25-FITC,","
R15824 T21171 T21174 arg1Of ",",and
R15825 T21172 T21171 arg2Of anti-CD62L-PE,","
R15826 T21174 T21169 arg2Of and,with
R15827 T21174 T21173 arg1Of and,","
R15828 T21174 T21176 arg1Of and,prior
R15829 T21175 T21174 arg2Of anti-CD4-APC,and
R15830 T21176 T21177 arg1Of prior,to
R15831 T21178 T21177 arg2Of sorting,to
R15832 T21180 T21179 arg1Of cells,Dead
R15833 T21180 T21181 arg1Of cells,were
R15834 T21180 T21182 arg2Of cells,excluded
R15835 T21182 T21181 arg2Of excluded,were
R15836 T21182 T21183 arg1Of excluded,with
R15837 T21184 T21183 arg2Of 4′,with
R15838 T21184 T21185 arg1Of 4′,'
R15839 T21184 T21186 arg1Of 4′,","
R15840 T21187 T21186 arg2Of 6-Diamidino-2-phenylindole,","
R15841 T21187 T21188 arg1Of 6-Diamidino-2-phenylindole,(
R15842 T21189 T21188 arg2Of DAPI,(
R15843 T21190 T21188 arg3Of ),(
R15844 T21192 T21191 arg1Of analyze,To
R15845 T21195 T21192 arg2Of expression,analyze
R15846 T21195 T21193 arg1Of expression,murine
R15847 T21195 T21194 arg1Of expression,Foxp3
R15848 T21195 T21196 arg1Of expression,in
R15849 T21199 T21196 arg2Of cultures,in
R15850 T21199 T21197 arg1Of cultures,inducible
R15851 T21199 T21198 arg1Of cultures,Treg
R15852 T21201 T21192 arg1Of cells,analyze
R15853 T21201 T21202 arg1Of cells,were
R15854 T21201 T21203 arg2Of cells,stained
R15855 T21203 T21191 modOf stained,To
R15856 T21203 T21200 arg1Of stained,","
R15857 T21203 T21202 arg2Of stained,were
R15858 T21203 T21204 arg1Of stained,intracellularly
R15859 T21203 T21205 arg1Of stained,with
R15860 T21203 T21207 arg1Of stained,according
R15861 T21203 T21212 arg1Of stained,","
R15862 T21203 T21213 arg1Of stained,in
R15863 T21206 T21205 arg2Of anti-Foxp3-PE,with
R15864 T21208 T21207 arg2Of to,according
R15865 T21209 T21210 arg2Of manufacturer,'s
R15866 T21211 T21208 arg2Of instruction,to
R15867 T21211 T21210 arg1Of instruction,'s
R15868 T21213 T21215 arg1Of in,with
R15869 T21214 T21213 arg2Of conjunction,in
R15870 T21216 T21217 arg1Of anti-CD4-APC,and
R15871 T21218 T21217 arg2Of LIVE/DEAD,and
R15872 T21223 T21213 arg3Of kit,in
R15873 T21223 T21216 arg1Of kit,anti-CD4-APC
R15874 T21223 T21218 arg1Of kit,LIVE/DEAD
R15875 T21223 T21219 arg1Of kit,fixable
R15876 T21223 T21220 arg1Of kit,dead
R15877 T21223 T21221 arg1Of kit,cell
R15878 T21223 T21222 arg1Of kit,stain
R15879 T21223 T21224 arg1Of kit,(
R15880 T21223 T21227 modOf kit,to
R15881 T21223 T21228 arg1Of kit,discriminate
R15882 T21225 T21224 arg2Of Invitrogen,(
R15883 T21226 T21224 arg3Of ),(
R15884 T21228 T21227 arg1Of discriminate,to
R15885 T21230 T21228 arg2Of cells,discriminate
R15886 T21230 T21229 arg1Of cells,live
R15887 T21233 T21231 arg1Of antibodies,All
R15888 T21233 T21232 arg1Of antibodies,monoclonal
R15889 T21233 T21234 arg1Of antibodies,for
R15890 T21233 T21238 arg1Of antibodies,were
R15891 T21233 T21239 arg2Of antibodies,purchased
R15892 T21237 T21234 arg2Of stainings,for
R15893 T21237 T21235 arg1Of stainings,murine
R15894 T21237 T21236 arg1Of stainings,cell
R15895 T21239 T21238 arg2Of purchased,were
R15896 T21239 T21240 arg1Of purchased,from
R15897 T21241 T21242 arg1Of eBioscience,or
R15898 T21242 T21240 arg2Of or,from
R15899 T21244 T21242 arg2Of Biosciences,or
R15900 T21244 T21243 arg1Of Biosciences,BD
R16212 T21791 T21790 arg1Of blotting,Western
R16213 T21794 T21792 arg2Of analysis,For
R16214 T21794 T21793 arg1Of analysis,FOXP3
R16215 T21794 T21795 arg1Of analysis,on
R16216 T21798 T21795 arg2Of level,on
R16217 T21798 T21796 arg1Of level,the
R16218 T21798 T21797 arg1Of level,protein
R16219 T21800 T21801 arg1Of 1,×
R16220 T21802 T21801 arg2Of 106,×
R16221 T21804 T21800 arg1Of cells,1
R16222 T21804 T21802 arg1Of cells,106
R16223 T21804 T21803 arg1Of cells,CD4+CD25–
R16224 T21804 T21805 arg1Of cells,were
R16225 T21804 T21806 arg2Of cells,lysed
R16226 T21804 T21808 arg2Of cells,loaded
R16227 T21806 T21807 arg1Of lysed,and
R16228 T21807 T21792 arg1Of and,For
R16229 T21807 T21799 arg1Of and,","
R16230 T21807 T21805 arg2Of and,were
R16231 T21807 T21819 arg1Of and,on
R16232 T21808 T21807 arg2Of loaded,and
R16233 T21808 T21810 arg1Of loaded,to
R16234 T21808 T21814 arg1Of loaded,(
R16235 T21810 T21809 arg1Of to,next
R16236 T21813 T21810 arg2Of ladder,to
R16237 T21813 T21811 arg1Of ladder,a
R16238 T21813 T21812 arg1Of ladder,protein-mass
R16239 T21815 T21814 arg2Of Magicmark,(
R16240 T21815 T21816 arg1Of Magicmark,","
R16241 T21815 T21817 arg1Of Magicmark,Invitrogen
R16242 T21818 T21814 arg3Of ),(
R16243 T21822 T21823 arg1Of 4–12,%
R16244 T21825 T21819 arg2Of gel,on
R16245 T21825 T21820 arg1Of gel,a
R16246 T21825 T21821 arg1Of gel,NuPAGE
R16247 T21825 T21822 arg1Of gel,4–12
R16248 T21825 T21824 arg1Of gel,bis-tris
R16249 T21825 T21826 arg1Of gel,(
R16250 T21827 T21826 arg2Of Invitrogen,(
R16251 T21828 T21826 arg3Of ),(
R16252 T21830 T21829 arg1Of proteins,The
R16253 T21830 T21831 arg1Of proteins,were
R16254 T21830 T21832 arg2Of proteins,electroblotted
R16255 T21832 T21831 arg2Of electroblotted,were
R16256 T21832 T21833 arg1Of electroblotted,onto
R16257 T21832 T21837 arg1Of electroblotted,(
R16258 T21836 T21833 arg2Of membrane,onto
R16259 T21836 T21834 arg1Of membrane,a
R16260 T21836 T21835 arg1Of membrane,PVDF
R16261 T21840 T21837 arg2Of Science,(
R16262 T21840 T21838 arg1Of Science,Amersham
R16263 T21840 T21839 arg1Of Science,Life
R16264 T21841 T21837 arg3Of ),(
R16265 T21843 T21842 arg1Of binding,Unspecific
R16266 T21843 T21844 arg1Of binding,was
R16267 T21843 T21845 arg2Of binding,blocked
R16268 T21845 T21844 arg2Of blocked,was
R16269 T21845 T21846 arg1Of blocked,with
R16270 T21845 T21849 arg1Of blocked,and
R16271 T21847 T21846 arg2Of BSA,with
R16272 T21849 T21848 arg1Of and,","
R16273 T21849 T21858 arg1Of and,:
R16274 T21851 T21850 arg1Of membranes,the
R16275 T21851 T21852 arg1Of membranes,were
R16276 T21851 T21854 arg2Of membranes,incubated
R16277 T21854 T21849 arg2Of incubated,and
R16278 T21854 T21852 arg2Of incubated,were
R16279 T21854 T21853 arg1Of incubated,subsequently
R16280 T21854 T21855 arg1Of incubated,with
R16281 T21857 T21855 arg2Of 1,with
R16282 T21857 T21856 arg1Of 1,an
R16283 T21860 T21859 arg1Of dilution,200
R16284 T21860 T21861 arg1Of dilution,of
R16285 T21860 T21864 arg1Of dilution,in
R16286 T21860 T21876 arg1Of dilution,were
R16287 T21860 T21877 arg2Of dilution,developed
R16288 T21860 T21888 arg2Of dilution,visualized
R16289 T21863 T21861 arg2Of anti-FOXP3,of
R16290 T21863 T21862 arg1Of anti-FOXP3,goat
R16291 T21865 T21864 arg2Of blocking,in
R16292 T21865 T21870 arg1Of blocking,overnight
R16293 T21865 T21871 arg1Of blocking,at
R16294 T21866 T21865 arg2Of buffer,blocking
R16295 T21866 T21867 arg1Of buffer,(
R16296 T21868 T21867 arg2Of Abcam,(
R16297 T21869 T21867 arg3Of ),(
R16298 T21873 T21872 arg1Of °C.,4
R16299 T21875 T21871 arg2Of blots,at
R16300 T21875 T21873 arg1Of blots,°C.
R16301 T21875 T21874 arg1Of blots,The
R16302 T21877 T21887 arg1Of developed,and
R16303 T21878 T21877 arg3Of using,developed
R16304 T21882 T21878 arg2Of mAb,using
R16305 T21882 T21879 arg1Of mAb,an
R16306 T21882 T21880 arg1Of mAb,anti-goat
R16307 T21882 T21881 arg1Of mAb,HRP-labeled
R16308 T21882 T21883 arg1Of mAb,(
R16309 T21885 T21883 arg2Of Biosciences,(
R16310 T21885 T21884 arg1Of Biosciences,Amersham
R16311 T21886 T21883 arg3Of ),(
R16312 T21887 T21858 arg2Of and,:
R16313 T21887 T21876 arg2Of and,were
R16314 T21888 T21887 arg2Of visualized,and
R16315 T21888 T21889 arg1Of visualized,with
R16316 T21893 T21889 arg2Of camera,with
R16317 T21893 T21890 arg1Of camera,a
R16318 T21893 T21891 arg1Of camera,LAS
R16319 T21893 T21892 arg1Of camera,1000
R16320 T21893 T21894 arg1Of camera,(
R16321 T21895 T21894 arg2Of Fuji,(
R16322 T21896 T21894 arg3Of ),(
R16323 T21897 T21898 arg1Of Membranes,were
R16324 T21897 T21899 arg2Of Membranes,incubated
R16325 T21897 T21904 arg1Of Membranes,re-blocked
R16326 T21899 T21900 arg1Of incubated,in
R16327 T21899 T21903 arg1Of incubated,and
R16328 T21901 T21900 arg2Of stripping,in
R16329 T21902 T21901 arg2Of buffer,stripping
R16330 T21903 T21898 arg2Of and,were
R16331 T21904 T21903 arg2Of re-blocked,and
R16332 T21904 T21905 arg1Of re-blocked,for
R16333 T21907 T21905 arg2Of h,for
R16334 T21907 T21906 arg1Of h,1
R16335 T21909 T21908 arg1Of membranes,The
R16336 T21909 T21910 arg1Of membranes,were
R16337 T21909 T21911 arg2Of membranes,re-probed
R16338 T21911 T21910 arg2Of re-probed,were
R16339 T21911 T21955 arg1Of re-probed,","
R16340 T21912 T21911 arg3Of using,re-probed
R16341 T21913 T21914 arg1Of anti-GATA3,(
R16342 T21913 T21921 arg1Of anti-GATA3,","
R16343 T21915 T21914 arg2Of HG3–31,(
R16344 T21915 T21916 arg1Of HG3–31,;
R16345 T21919 T21916 arg2Of Biotechnology,;
R16346 T21919 T21917 arg1Of Biotechnology,Santa
R16347 T21919 T21918 arg1Of Biotechnology,Cruz
R16348 T21920 T21914 arg3Of ),(
R16349 T21921 T21948 arg1Of ",",and
R16350 T21922 T21923 arg1Of anti-T-bet,(
R16351 T21922 T21930 arg1Of anti-T-bet,","
R16352 T21924 T21923 arg2Of 4B10,(
R16353 T21924 T21925 arg1Of 4B10,","
R16354 T21928 T21925 arg2Of Biotechnology,","
R16355 T21928 T21926 arg1Of Biotechnology,Santa
R16356 T21928 T21927 arg1Of Biotechnology,Cruz
R16357 T21929 T21923 arg3Of ),(
R16358 T21930 T21921 arg2Of ",",","
R16359 T21931 T21932 arg1Of anti-GAPDH,(
R16360 T21931 T21937 arg1Of anti-GAPDH,","
R16361 T21933 T21932 arg2Of 6C5,(
R16362 T21933 T21934 arg1Of 6C5,","
R16363 T21933 T21935 arg1Of 6C5,Ambion
R16364 T21936 T21932 arg3Of ),(
R16365 T21937 T21942 arg1Of ",",","
R16366 T21938 T21937 arg2Of anti-phospho-SMAD2,","
R16367 T21938 T21939 arg1Of anti-phospho-SMAD2,(
R16368 T21940 T21939 arg2Of 138D4,(
R16369 T21941 T21939 arg3Of ),(
R16370 T21942 T21930 arg2Of ",",","
R16371 T21943 T21942 arg2Of anti-phospho-STAT6,","
R16372 T21943 T21944 arg1Of anti-phospho-STAT6,(
R16373 T21945 T21944 arg2Of 5A4,(
R16374 T21946 T21944 arg3Of ),(
R16375 T21948 T21912 arg2Of and,using
R16376 T21948 T21947 arg1Of and,","
R16377 T21949 T21948 arg2Of anti-STAT6,and
R16378 T21949 T21950 arg1Of anti-STAT6,(
R16379 T21953 T21950 arg2Of Technology,(
R16380 T21953 T21951 arg1Of Technology,Cell
R16381 T21953 T21952 arg1Of Technology,Signaling
R16382 T21954 T21950 arg3Of ),(
R16570 T22260 T22261 arg1Of Administration,of
R16571 T22262 T22263 arg1Of cytokines,and
R16572 T22263 T22261 arg2Of and,of
R16573 T22264 T22263 arg2Of antibodies,and
R16574 T22264 T22266 arg1Of antibodies,vivo
R16575 T22266 T22265 arg1Of vivo,in
R16576 T22272 T22267 arg1Of mice,Age-
R16577 T22272 T22268 arg1Of mice,and
R16578 T22272 T22269 arg1Of mice,gender-matched
R16579 T22272 T22270 arg1Of mice,normal
R16580 T22272 T22271 arg1Of mice,B6
R16581 T22272 T22273 arg1Of mice,received
R16582 T22273 T22276 arg1Of received,day
R16583 T22273 T22316 arg1Of received,for
R16584 T22276 T22274 arg1Of day,every
R16585 T22276 T22275 arg1Of day,other
R16586 T22277 T22278 arg1Of intraperitoneal,(
R16587 T22279 T22278 arg2Of ip,(
R16588 T22280 T22278 arg3Of ),(
R16589 T22281 T22277 arg1Of injections,intraperitoneal
R16590 T22281 T22282 arg1Of injections,of
R16591 T22281 T22299 arg1Of injections,or
R16592 T22283 T22282 arg2Of PBS,of
R16593 T22283 T22284 arg1Of PBS,","
R16594 T22287 T22284 arg2Of rmIL-4,","
R16595 T22287 T22285 arg1Of rmIL-4,1.5
R16596 T22287 T22286 arg1Of rmIL-4,μg
R16597 T22287 T22288 arg1Of rmIL-4,","
R16598 T22289 T22290 arg1Of 50,μg
R16599 T22290 T22288 arg2Of μg,","
R16600 T22290 T22293 arg1Of μg,(
R16601 T22292 T22290 arg2Of mAb,μg
R16602 T22292 T22291 arg1Of mAb,anti-IL-4
R16603 T22294 T22295 arg1Of 11B11,or
R16604 T22295 T22293 arg2Of or,(
R16605 T22296 T22295 arg2Of MAB404,or
R16606 T22297 T22293 arg3Of ),(
R16607 T22299 T22298 arg1Of or,","
R16608 T22299 T22306 arg1Of or,plus
R16609 T22301 T22299 arg2Of mixture,or
R16610 T22301 T22300 arg1Of mixture,a
R16611 T22301 T22302 arg1Of mixture,of
R16612 T22303 T22304 arg1Of 1.5,μg
R16613 T22305 T22302 arg2Of rmIL-4,of
R16614 T22305 T22303 arg1Of rmIL-4,1.5
R16615 T22306 T22273 arg2Of plus,received
R16616 T22307 T22308 arg1Of 50,μg
R16617 T22309 T22308 arg2Of anti-IL-4,μg
R16618 T22310 T22306 arg2Of mAb,plus
R16619 T22310 T22307 arg1Of mAb,50
R16620 T22310 T22309 arg1Of mAb,anti-IL-4
R16621 T22310 T22311 arg1Of mAb,(
R16622 T22312 T22313 arg1Of 11B11,or
R16623 T22313 T22311 arg2Of or,(
R16624 T22314 T22313 arg2Of MAB404,or
R16625 T22315 T22311 arg3Of ),(
R16626 T22318 T22316 arg2Of d,for
R16627 T22318 T22317 arg1Of d,7
R16628 T22321 T22322 arg1Of spleen,and
R16629 T22323 T22322 arg2Of lymph,and
R16630 T22325 T22321 arg1Of cells,spleen
R16631 T22325 T22323 arg1Of cells,lymph
R16632 T22325 T22324 arg1Of cells,node
R16633 T22325 T22326 arg1Of cells,were
R16634 T22325 T22327 arg2Of cells,analyzed
R16635 T22327 T22319 arg1Of analyzed,Thereafter
R16636 T22327 T22320 arg1Of analyzed,","
R16637 T22327 T22326 arg2Of analyzed,were
R16638 T22327 T22328 arg1Of analyzed,by
R16639 T22330 T22328 arg2Of cytometry,by
R16640 T22330 T22329 arg1Of cytometry,flow
R16641 T22330 T22331 arg1Of cytometry,for
R16642 T22332 T22333 arg1Of CD3,","
R16643 T22333 T22336 arg1Of ",",and
R16644 T22334 T22333 arg2Of CD4,","
R16645 T22336 T22331 arg2Of and,for
R16646 T22336 T22335 arg1Of and,","
R16647 T22338 T22336 arg2Of expression,and
R16648 T22338 T22337 arg1Of expression,CD25
R16649 T22343 T22339 arg1Of MAB404,The
R16650 T22343 T22340 arg1Of MAB404,anti-mouse
R16651 T22343 T22341 arg1Of MAB404,IL-4
R16652 T22343 T22342 arg1Of MAB404,mAb
R16653 T22343 T22344 arg1Of MAB404,was
R16654 T22343 T22345 arg2Of MAB404,obtained
R16655 T22345 T22344 arg2Of obtained,was
R16656 T22345 T22346 arg1Of obtained,from
R16657 T22350 T22346 arg2Of Systems,from
R16658 T22350 T22347 arg1Of Systems,R
R16659 T22350 T22348 arg1Of Systems,&
R16660 T22350 T22349 arg1Of Systems,D
R16661 T22357 T22352 arg1Of 11B11,the
R16662 T22357 T22353 arg1Of 11B11,second
R16663 T22357 T22354 arg1Of 11B11,anti-mouse
R16664 T22357 T22355 arg1Of 11B11,IL-4
R16665 T22357 T22356 arg1Of 11B11,mAb
R16666 T22357 T22358 arg1Of 11B11,was
R16667 T22357 T22359 arg2Of 11B11,purchased
R16668 T22359 T22344 modOf purchased,was
R16669 T22359 T22351 arg1Of purchased,","
R16670 T22359 T22358 arg2Of purchased,was
R16671 T22359 T22360 arg1Of purchased,from
R16672 T22361 T22360 arg2Of eBioscience,from
R16797 T22576 T22575 arg1Of analysis,ChIP
R16798 T22576 T22577 arg1Of analysis,was
R16799 T22576 T22578 arg2Of analysis,performed
R16800 T22578 T22577 arg2Of performed,was
R16801 T22578 T22579 arg1Of performed,according
R16802 T22578 T22594 arg1Of performed,and
R16803 T22580 T22579 arg2Of to,according
R16804 T22582 T22581 arg1Of manufacturer,the
R16805 T22582 T22583 arg2Of manufacturer,'s
R16806 T22584 T22580 arg2Of protocol,to
R16807 T22584 T22583 arg1Of protocol,'s
R16808 T22584 T22585 arg1Of protocol,(
R16809 T22584 T22589 arg1Of protocol,with
R16810 T22587 T22585 arg2Of Biotechnology,(
R16811 T22587 T22586 arg1Of Biotechnology,Upstate
R16812 T22588 T22585 arg3Of ),(
R16813 T22593 T22589 arg2Of iTreg,with
R16814 T22593 T22590 arg1Of iTreg,the
R16815 T22593 T22591 arg1Of iTreg,following
R16816 T22593 T22592 arg1Of iTreg,modifications.
R16817 T22596 T22595 arg1Of cells,Th2
R16818 T22596 T22597 arg1Of cells,were
R16819 T22596 T22598 arg2Of cells,fixed
R16820 T22598 T22594 arg2Of fixed,and
R16821 T22598 T22597 arg2Of fixed,were
R16822 T22598 T22599 arg1Of fixed,with
R16823 T22598 T22603 arg1Of fixed,for
R16824 T22600 T22601 arg1Of 1,%
R16825 T22602 T22599 arg2Of formaldehyde,with
R16826 T22602 T22600 arg1Of formaldehyde,1
R16827 T22605 T22603 arg2Of min,for
R16828 T22605 T22604 arg1Of min,10
R16829 T22605 T22606 arg1Of min,at
R16830 T22608 T22606 arg2Of temperature,at
R16831 T22608 T22607 arg1Of temperature,room
R16832 T22610 T22609 arg1Of chromatin,The
R16833 T22610 T22611 arg1Of chromatin,was
R16834 T22610 T22612 arg2Of chromatin,sheared
R16835 T22612 T22611 arg2Of sheared,was
R16836 T22612 T22613 arg1Of sheared,to
R16837 T22612 T22620 arg1Of sheared,with
R16838 T22615 T22613 arg2Of bp,to
R16839 T22615 T22614 arg1Of bp,200-1000
R16840 T22615 T22616 arg1Of bp,of
R16841 T22617 T22616 arg2Of length,of
R16842 T22617 T22618 arg1Of length,by
R16843 T22619 T22618 arg2Of sonication,by
R16844 T22622 T22620 arg2Of pulses,with
R16845 T22622 T22621 arg1Of pulses,five
R16846 T22622 T22623 arg1Of pulses,of
R16847 T22625 T22623 arg2Of s,of
R16848 T22625 T22624 arg1Of s,10
R16849 T22625 T22626 arg1Of s,at
R16850 T22627 T22628 arg1Of 30,%
R16851 T22629 T22626 arg2Of power,at
R16852 T22629 T22627 arg1Of power,30
R16853 T22629 T22630 arg1Of power,(
R16854 T22631 T22630 arg2Of Bandelin,(
R16855 T22632 T22630 arg3Of ),(
R16856 T22634 T22633 arg1Of chromatin,The
R16857 T22634 T22635 arg1Of chromatin,was
R16858 T22634 T22636 arg1Of chromatin,pre-cleared
R16859 T22634 T22647 arg1Of chromatin,incubated
R16860 T22635 T22645 arg1Of was,and
R16861 T22636 T22635 arg2Of pre-cleared,was
R16862 T22636 T22637 arg1Of pre-cleared,for
R16863 T22639 T22637 arg2Of h,for
R16864 T22639 T22638 arg1Of h,2
R16865 T22639 T22640 arg1Of h,with
R16866 T22644 T22640 arg2Of beads,with
R16867 T22644 T22641 arg1Of beads,normal
R16868 T22644 T22642 arg1Of beads,mouse
R16869 T22644 T22643 arg1Of beads,IgG
R16870 T22647 T22645 arg2Of incubated,and
R16871 T22647 T22646 arg1Of incubated,then
R16872 T22647 T22648 arg1Of incubated,with
R16873 T22647 T22658 arg1Of incubated,for
R16874 T22650 T22648 arg2Of beads,with
R16875 T22650 T22649 arg1Of beads,anti-GATA3-agarose
R16876 T22650 T22651 arg1Of beads,(
R16877 T22652 T22651 arg2Of HG3–31,(
R16878 T22652 T22653 arg1Of HG3–31,;
R16879 T22656 T22653 arg2Of Biotechnology,;
R16880 T22656 T22654 arg1Of Biotechnology,Santa
R16881 T22656 T22655 arg1Of Biotechnology,Cruz
R16882 T22657 T22651 arg3Of ),(
R16883 T22660 T22658 arg2Of h,for
R16884 T22660 T22659 arg1Of h,2
R16885 T22661 T22675 arg1Of Washing,were
R16886 T22661 T22676 arg2Of Washing,reversed
R16887 T22664 T22662 arg1Of buffers,and
R16888 T22664 T22663 arg1Of buffers,elution
R16889 T22664 T22665 arg1Of buffers,were
R16890 T22664 T22666 arg2Of buffers,used
R16891 T22666 T22665 arg2Of used,were
R16892 T22666 T22667 arg1Of used,according
R16893 T22668 T22667 arg2Of to,according
R16894 T22670 T22668 arg2Of protocol,to
R16895 T22670 T22669 arg1Of protocol,the
R16896 T22670 T22671 arg1Of protocol,of
R16897 T22674 T22671 arg2Of Crosslinks,of
R16898 T22674 T22672 arg1Of Crosslinks,Upstate
R16899 T22674 T22673 arg1Of Crosslinks,Biotechnology.
R16900 T22676 T22665 modOf reversed,were
R16901 T22676 T22675 arg2Of reversed,were
R16902 T22676 T22679 arg1Of reversed,at
R16903 T22676 T22682 arg1Of reversed,for
R16904 T22676 T22685 arg1Of reversed,in
R16905 T22676 T22693 arg1Of reversed,and
R16906 T22678 T22676 arg1Of incubation,reversed
R16907 T22678 T22677 arg2Of incubation,by
R16908 T22681 T22679 arg2Of °C,at
R16909 T22681 T22680 arg1Of °C,65
R16910 T22684 T22682 arg2Of h,for
R16911 T22684 T22683 arg1Of h,4
R16912 T22687 T22685 arg2Of presence,in
R16913 T22687 T22686 arg1Of presence,the
R16914 T22687 T22688 arg1Of presence,of
R16915 T22689 T22690 arg1Of 0.2,M
R16916 T22691 T22688 arg2Of NaCl,of
R16917 T22691 T22689 arg1Of NaCl,0.2
R16918 T22693 T22692 arg1Of and,","
R16919 T22695 T22694 arg1Of DNA,the
R16920 T22695 T22696 arg1Of DNA,was
R16921 T22695 T22697 arg2Of DNA,purified
R16922 T22697 T22693 arg2Of purified,and
R16923 T22697 T22696 arg2Of purified,was
R16924 T22700 T22697 arg1Of extraction,purified
R16925 T22700 T22698 arg2Of extraction,by
R16926 T22700 T22699 arg1Of extraction,phenol/chloroform
R16927 T22702 T22701 arg1Of amount,The
R16928 T22702 T22703 arg1Of amount,of
R16929 T22702 T22705 arg1Of amount,was
R16930 T22702 T22706 arg2Of amount,determined
R16931 T22704 T22703 arg2Of DNA,of
R16932 T22706 T22705 arg2Of determined,was
R16933 T22709 T22706 arg1Of PCR,determined
R16934 T22709 T22707 arg2Of PCR,by
R16935 T22709 T22708 arg1Of PCR,conventional
R16936 T22711 T22710 arg1Of PCR,The
R16937 T22711 T22712 arg2Of PCR,addressed
R16938 T22711 T22722 arg1Of PCR,was
R16939 T22711 T22723 arg2Of PCR,performed
R16940 T22712 T22713 arg1Of addressed,for
R16941 T22717 T22713 arg2Of region,for
R16942 T22717 T22714 arg1Of region,the
R16943 T22717 T22715 arg1Of region,FOXP3
R16944 T22717 T22716 arg1Of region,promoter
R16945 T22717 T22718 arg1Of region,−246
R16946 T22717 T22719 arg1Of region,to
R16947 T22720 T22719 arg2Of −511,to
R16948 T22720 T22721 arg1Of −511,and
R16949 T22723 T22722 arg2Of performed,was
R16950 T22724 T22723 arg3Of using,performed
R16951 T22727 T22724 arg2Of primers,using
R16952 T22727 T22725 arg1Of primers,the
R16953 T22727 T22726 arg1Of primers,following
R16954 T22727 T22728 arg1Of primers,:
R16955 T22730 T22731 arg1Of catctg-3′,and
R16956 T22731 T22729 arg1Of and,5′-gtgccctttacgagt
R16957 T22732 T22731 arg2Of 5′-gtgccctttacgagtcatctg-3′,and
R16958 T22735 T22733 arg1Of products,The
R16959 T22735 T22734 arg1Of products,PCR
R16960 T22735 T22736 arg1Of products,were
R16961 T22735 T22737 arg2Of products,visualized
R16962 T22737 T22736 arg2Of visualized,were
R16963 T22738 T22737 arg3Of using,visualized
R16964 T22742 T22738 arg2Of gel,using
R16965 T22742 T22739 arg1Of gel,an
R16966 T22742 T22740 arg1Of gel,ethidium
R16967 T22742 T22741 arg1Of gel,bromide
R17158 T22976 T22975 arg1Of assay,Pull-down
R17159 T22979 T22977 arg1Of cells,CD4+
R17160 T22979 T22978 arg1Of cells,T
R17161 T22979 T22980 arg1Of cells,were
R17162 T22979 T22981 arg2Of cells,stimulated
R17163 T22981 T22980 arg2Of stimulated,were
R17164 T22981 T22982 arg1Of stimulated,with
R17165 T22983 T22984 arg1Of PMA,and
R17166 T22984 T22982 arg2Of and,with
R17167 T22984 T22986 arg1Of and,for
R17168 T22984 T22989 arg1Of and,at
R17169 T22985 T22984 arg2Of ionomycin,and
R17170 T22988 T22986 arg2Of h,for
R17171 T22988 T22987 arg1Of h,2
R17172 T22990 T22989 arg2Of 37°C.,at
R17173 T22992 T22991 arg1Of cells,The
R17174 T22992 T22993 arg1Of cells,were
R17175 T22992 T22994 arg2Of cells,pelleted
R17176 T22992 T22996 arg2Of cells,resuspended
R17177 T22992 T23036 arg2Of cells,) and
R17178 T22994 T22995 arg1Of pelleted,","
R17179 T22995 T23000 arg1Of ",",(
R17180 T22995 T23035 arg1Of ",",-40
R17181 T22996 T22995 arg2Of resuspended,","
R17182 T22996 T22997 arg1Of resuspended,in
R17183 T22999 T22997 arg2Of C,in
R17184 T22999 T22998 arg1Of C,buffer
R17185 T23001 T23002 arg1Of 20,mM
R17186 T23003 T23001 arg1Of HEPES,20
R17187 T23003 T23004 arg1Of HEPES,[
R17188 T23003 T23008 arg1Of HEPES,","
R17189 T23005 T23004 arg2Of pH,[
R17190 T23005 T23006 arg1Of pH,7.9
R17191 T23007 T23004 arg3Of ],[
R17192 T23008 T23024 arg1Of ",",","
R17193 T23009 T23010 arg1Of 420,mM
R17194 T23011 T23009 arg1Of NaCl,420
R17195 T23011 T23012 arg1Of NaCl,","
R17196 T23012 T23016 arg1Of ",",","
R17197 T23013 T23014 arg1Of 1.5,mM
R17198 T23015 T23012 arg2Of MgCl2,","
R17199 T23015 T23013 arg1Of MgCl2,1.5
R17200 T23016 T23020 arg1Of ",",","
R17201 T23017 T23018 arg1Of 0.2,mM
R17202 T23019 T23016 arg2Of EDTA,","
R17203 T23019 T23017 arg1Of EDTA,0.2
R17204 T23020 T23008 arg2Of ",",","
R17205 T23021 T23022 arg1Of 1,mM
R17206 T23023 T23020 arg2Of DTT,","
R17207 T23023 T23021 arg1Of DTT,1
R17208 T23024 T23030 arg1Of ",",. a
R17209 T23026 T23024 arg2Of inhibitors,","
R17210 T23026 T23025 arg1Of inhibitors,protease
R17211 T23026 T23027 arg1Of inhibitors,[
R17212 T23028 T23027 arg2Of Sigma,[
R17213 T23029 T23027 arg3Of ],[
R17214 T23030 T23000 arg2Of . a,(
R17215 T23031 T23032 arg1Of nd ,0
R17216 T23033 T23030 arg2Of .1% N,. a
R17217 T23033 T23031 arg1Of .1% N,nd
R17218 T23034 T23000 arg3Of P,(
R17219 T23035 T22980 modOf -40,were
R17220 T23035 T22993 arg2Of -40,were
R17221 T23036 T23035 arg2Of ) and,-40
R17222 T23036 T23037 arg1Of ) and,ly
R17223 T23036 T23039 arg1Of ) and,on
R17224 T23038 T23037 arg2Of sed,ly
R17225 T23041 T23039 arg2Of e f,on
R17226 T23041 T23040 arg1Of e f,ic
R17227 T23043 T23042 arg1Of material,Insoluble
R17228 T23043 T23044 arg1Of material,was
R17229 T23043 T23045 arg2Of material,removed
R17230 T23045 T23044 arg2Of removed,was
R17231 T23047 T23045 arg1Of centrifugation,removed
R17232 T23047 T23046 arg2Of centrifugation,by
R17233 T23049 T23048 arg1Of supernatant,The
R17234 T23049 T23050 arg1Of supernatant,was
R17235 T23049 T23051 arg2Of supernatant,diluted
R17236 T23051 T23050 arg2Of diluted,was
R17237 T23051 T23052 arg1Of diluted,1
R17238 T23052 T23053 arg1Of 1,:
R17239 T23054 T23053 arg2Of 3,:
R17240 T23054 T23055 arg1Of 3,with
R17241 T23054 T23058 arg1Of 3,(
R17242 T23057 T23055 arg2Of D,with
R17243 T23057 T23056 arg1Of D,buffer
R17244 T23059 T23063 arg1Of as,but
R17245 T23061 T23059 arg2Of C,as
R17246 T23061 T23060 arg1Of C,buffer
R17247 T23063 T23058 arg2Of but,(
R17248 T23063 T23062 arg1Of but,","
R17249 T23064 T23063 arg2Of without,but
R17250 T23065 T23064 arg2Of NaCl,without
R17251 T23066 T23058 arg3Of ),(
R17252 T23068 T23067 arg1Of lysates,The
R17253 T23068 T23069 arg1Of lysates,were
R17254 T23068 T23070 arg2Of lysates,incubated
R17255 T23068 T23117 arg1Of lysates,resuspended
R17256 T23070 T23071 arg1Of incubated,with
R17257 T23070 T23098 arg1Of incubated,at
R17258 T23070 T23116 arg1Of incubated,and
R17259 T23073 T23071 arg2Of μg,with
R17260 T23073 T23072 arg1Of μg,10
R17261 T23073 T23074 arg1Of μg,of
R17262 T23075 T23076 arg1Of poly,(
R17263 T23075 T23079 arg1Of poly,(
R17264 T23075 T23082 arg1Of poly,and
R17265 T23077 T23076 arg2Of dI-dC,(
R17266 T23078 T23076 arg3Of ),(
R17267 T23080 T23079 arg2Of Sigma,(
R17268 T23081 T23079 arg3Of ),(
R17269 T23082 T23074 arg2Of and,of
R17270 T23084 T23082 arg2Of μl,and
R17271 T23084 T23083 arg1Of μl,70
R17272 T23084 T23085 arg1Of μl,of
R17273 T23089 T23087 arg2Of Biosciences,(
R17274 T23089 T23088 arg1Of Biosciences,Amersham
R17275 T23090 T23087 arg3Of ),(
R17276 T23093 T23085 arg2Of oligonucleotides,of
R17277 T23093 T23086 arg1Of oligonucleotides,streptavidin-agarose
R17278 T23093 T23087 arg1Of oligonucleotides,(
R17279 T23093 T23091 arg1Of oligonucleotides,carrying
R17280 T23093 T23092 arg2Of oligonucleotides,biotinylated
R17281 T23093 T23094 arg1Of oligonucleotides,","
R17282 T23093 T23095 arg1Of oligonucleotides,for
R17283 T23097 T23095 arg2Of h,for
R17284 T23097 T23096 arg1Of h,3
R17285 T23100 T23099 arg1Of °C.,4
R17286 T23100 T23103 modOf °C.,were
R17287 T23102 T23101 arg1Of beads,The
R17288 T23102 T23103 arg1Of beads,were
R17289 T23102 T23104 arg2Of beads,washed
R17290 T23104 T23103 arg2Of washed,were
R17291 T23104 T23105 arg1Of washed,twice
R17292 T23104 T23106 arg1Of washed,with
R17293 T23104 T23109 arg1Of washed,:
R17294 T23108 T23106 arg2Of C,with
R17295 T23108 T23107 arg1Of C,buffer
R17296 T23110 T23098 arg2Of D,at
R17297 T23110 T23100 arg1Of D,°C.
R17298 T23110 T23111 arg1Of D,(
R17299 T23112 T23113 arg1Of 1,:
R17300 T23113 T23111 arg2Of :,(
R17301 T23114 T23113 arg2Of 3,:
R17302 T23115 T23111 arg3Of ),(
R17303 T23116 T23069 arg2Of and,were
R17304 T23117 T23116 arg2Of resuspended,and
R17305 T23117 T23118 arg1Of resuspended,in
R17306 T23121 T23119 arg1Of buffer,DTT-containing
R17307 T23121 T23120 arg1Of buffer,loading
R17308 T23121 T23122 arg1Of buffer,(
R17309 T23121 T23127 arg1Of buffer,","
R17310 T23121 T23128 arg2Of buffer,heated
R17311 T23121 T23136 arg1Of buffer,and
R17312 T23123 T23122 arg2Of NuPAGE,(
R17313 T23123 T23124 arg1Of NuPAGE,;
R17314 T23125 T23124 arg2Of Invitrogen,;
R17315 T23126 T23122 arg3Of ),(
R17316 T23128 T23129 arg1Of heated,to
R17317 T23128 T23132 arg1Of heated,for
R17318 T23131 T23129 arg2Of °C,to
R17319 T23131 T23130 arg1Of °C,70
R17320 T23134 T23132 arg2Of min,for
R17321 T23134 T23133 arg1Of min,10
R17322 T23136 T23118 arg2Of and,in
R17323 T23136 T23135 arg1Of and,","
R17324 T23138 T23136 arg2Of eluants,and
R17325 T23138 T23137 arg1Of eluants,the
R17326 T23138 T23139 arg1Of eluants,on
R17327 T23142 T23143 arg1Of 4–12,%
R17328 T23145 T23139 arg2Of gel,on
R17329 T23145 T23140 arg1Of gel,a
R17330 T23145 T23141 arg1Of gel,NuPAGE
R17331 T23145 T23142 arg1Of gel,4–12
R17332 T23145 T23144 arg1Of gel,bis-tris
R17333 T23145 T23146 arg1Of gel,(
R17334 T23147 T23146 arg2Of Invitrogen,(
R17335 T23148 T23146 arg3Of ),(
R17336 T23150 T23149 arg1Of proteins,The
R17337 T23150 T23151 arg1Of proteins,were
R17338 T23150 T23152 arg2Of proteins,electroblotted
R17339 T23150 T23162 arg2Of proteins,detected
R17340 T23150 T23163 arg1Of proteins,using
R17341 T23152 T23153 arg1Of electroblotted,onto
R17342 T23152 T23161 arg1Of electroblotted,and
R17343 T23156 T23153 arg2Of membrane,onto
R17344 T23156 T23154 arg1Of membrane,a
R17345 T23156 T23155 arg1Of membrane,PVDF
R17346 T23156 T23157 arg1Of membrane,(
R17347 T23159 T23157 arg2Of Biosciences,(
R17348 T23159 T23158 arg1Of Biosciences,Amersham
R17349 T23160 T23157 arg3Of ),(
R17350 T23161 T23151 arg2Of and,were
R17351 T23162 T23161 arg2Of detected,and
R17352 T23163 T23162 arg3Of using,detected
R17353 T23166 T23163 arg2Of mAb,using
R17354 T23166 T23164 arg1Of mAb,an
R17355 T23166 T23165 arg1Of mAb,anti-GATA3
R17356 T23166 T23167 arg1Of mAb,(
R17357 T23170 T23167 arg2Of Biotechnology,(
R17358 T23170 T23168 arg1Of Biotechnology,Santa
R17359 T23170 T23169 arg1Of Biotechnology,Cruz
R17360 T23171 T23167 arg3Of ),(
R17361 T23173 T23172 arg2Of signals,Accumulated
R17362 T23173 T23174 arg1Of signals,were
R17363 T23173 T23175 arg2Of signals,analyzed
R17364 T23175 T23174 arg2Of analyzed,were
R17365 T23176 T23175 arg3Of using,analyzed
R17366 T23178 T23176 arg2Of software,using
R17367 T23178 T23177 arg1Of software,AIDA
R17368 T23178 T23179 arg1Of software,(
R17369 T23180 T23179 arg2Of Raytest,(
R17370 T23181 T23179 arg3Of ),(
R17595 T23465 T23464 arg1Of Cells,Th2
R17596 T23465 T23466 arg1Of Cells,Can
R17597 T23465 T23468 arg1Of Cells,Induce
R17598 T23468 T23466 arg2Of Induce,Can
R17599 T23468 T23467 arg1Of Induce,not
R17600 T23470 T23468 arg2Of Expression,Induce
R17601 T23470 T23469 arg1Of Expression,FOXP3
R17602 T23472 T23471 arg2Of A,(
R17603 T23473 T23471 arg3Of ),(
R17604 T23476 T23471 arg1Of cells,(
R17605 T23476 T23474 arg1Of cells,Human
R17606 T23476 T23475 arg1Of cells,T
R17607 T23476 T23477 arg1Of cells,were
R17608 T23476 T23478 arg2Of cells,activated
R17609 T23478 T23477 arg2Of activated,were
R17610 T23478 T23479 arg1Of activated,with
R17611 T23478 T23482 arg1Of activated,with
R17612 T23478 T23484 arg1Of activated,without
R17613 T23478 T23486 arg1Of activated,as
R17614 T23481 T23479 arg2Of anti-CD3/CD28,with
R17615 T23481 T23480 arg1Of anti-CD3/CD28,plate-bound
R17616 T23482 T23483 arg1Of with,or
R17617 T23484 T23483 arg2Of without,or
R17618 T23485 T23482 arg2Of TGF-β,with
R17619 T23485 T23484 arg2Of TGF-β,without
R17620 T23487 T23486 arg2Of indicated,as
R17621 T23487 T23488 arg1Of indicated,on
R17622 T23490 T23488 arg2Of x-axis,on
R17623 T23490 T23489 arg1Of x-axis,the
R17624 T23490 T23491 arg1Of x-axis,of
R17625 T23490 T23492 arg1Of x-axis,(
R17626 T23493 T23492 arg2Of B,(
R17627 T23494 T23492 arg3Of ),(
R17628 T23495 T23496 arg1Of Cells,were
R17629 T23495 T23497 arg2Of Cells,harvested
R17630 T23497 T23496 arg2Of harvested,were
R17631 T23497 T23498 arg1Of harvested,after
R17632 T23497 T23501 arg1Of harvested,and
R17633 T23500 T23498 arg2Of days,after
R17634 T23500 T23499 arg1Of days,5
R17635 T23503 T23502 arg1Of mRNA,FOXP3
R17636 T23503 T23504 arg1Of mRNA,was
R17637 T23503 T23505 arg2Of mRNA,quantified
R17638 T23505 T23501 arg2Of quantified,and
R17639 T23505 T23504 arg2Of quantified,was
R17640 T23508 T23505 arg1Of PCR,quantified
R17641 T23508 T23506 arg2Of PCR,by
R17642 T23508 T23507 arg1Of PCR,real-time
R17643 T23509 T23510 arg1Of Bars,show
R17644 T23514 T23510 arg2Of SD,show
R17645 T23514 T23511 arg1Of SD,the
R17646 T23514 T23512 arg1Of SD,mean
R17647 T23514 T23513 arg1Of SD,±
R17648 T23514 T23515 arg1Of SD,of
R17649 T23518 T23515 arg2Of experiments,of
R17650 T23518 T23516 arg1Of experiments,4
R17651 T23518 T23517 arg1Of experiments,independent
R17652 T23520 T23519 arg2Of B,(
R17653 T23521 T23519 arg3Of ),(
R17654 T23523 T23522 arg1Of vitro,In
R17655 T23524 T23523 arg1Of differentiated,vitro
R17656 T23525 T23526 arg1Of Th1,","
R17657 T23526 T23529 arg1Of ",",or
R17658 T23527 T23526 arg2Of Th2,","
R17659 T23529 T23528 arg1Of or,","
R17660 T23530 T23529 arg2Of iTreg,or
R17661 T23531 T23519 arg1Of cells,(
R17662 T23531 T23524 arg1Of cells,differentiated
R17663 T23531 T23525 arg1Of cells,Th1
R17664 T23531 T23527 arg1Of cells,Th2
R17665 T23531 T23530 arg1Of cells,iTreg
R17666 T23531 T23532 arg1Of cells,were
R17667 T23531 T23533 arg2Of cells,activated
R17668 T23533 T23532 arg2Of activated,were
R17669 T23533 T23534 arg1Of activated,with
R17670 T23533 T23541 arg1Of activated,as
R17671 T23535 T23536 arg1Of anti-CD3/CD28,","
R17672 T23536 T23539 arg1Of ",",or
R17673 T23537 T23536 arg2Of TGF-β,","
R17674 T23539 T23534 arg2Of or,with
R17675 T23539 T23538 arg1Of or,","
R17676 T23540 T23539 arg2Of anti-IL-4,or
R17677 T23542 T23541 arg2Of indicated,as
R17678 T23544 T23543 arg1Of phenotype,The
R17679 T23544 T23545 arg1Of phenotype,of
R17680 T23544 T23548 arg1Of phenotype,was
R17681 T23544 T23549 arg2Of phenotype,confirmed
R17682 T23547 T23545 arg2Of cells,of
R17683 T23547 T23546 arg1Of cells,these
R17684 T23549 T23548 arg2Of confirmed,was
R17685 T23551 T23552 arg1Of FACS,and
R17686 T23553 T23552 arg2Of proliferation,and
R17687 T23554 T23549 arg1Of analysis,confirmed
R17688 T23554 T23550 arg2Of analysis,by
R17689 T23554 T23551 arg1Of analysis,FACS
R17690 T23554 T23553 arg1Of analysis,proliferation
R17691 T23554 T23555 arg1Of analysis,(
R17692 T23557 T23555 arg2Of S1,(
R17693 T23557 T23556 arg1Of S1,Figure
R17694 T23558 T23555 arg3Of ),(
R17695 T23559 T23560 arg1Of Bars,show
R17696 T23564 T23560 arg2Of SD,show
R17697 T23564 T23561 arg1Of SD,the
R17698 T23564 T23562 arg1Of SD,mean
R17699 T23564 T23563 arg1Of SD,±
R17700 T23564 T23565 arg1Of SD,of
R17701 T23568 T23565 arg2Of experiments,of
R17702 T23568 T23566 arg1Of experiments,four
R17703 T23568 T23567 arg1Of experiments,independent
R17844 T23816 T23815 arg1Of Cells,Th2
R17845 T23816 T23817 arg1Of Cells,Do
R17846 T23819 T23818 arg1Of Express,Not
R17847 T23820 T23817 arg2Of FOXP3,Do
R17848 T23820 T23819 arg1Of FOXP3,Express
R17849 T23822 T23821 arg2Of A,(
R17850 T23823 T23821 arg3Of ),(
R17851 T23826 T23821 arg1Of analysis,(
R17852 T23826 T23824 arg1Of analysis,Intracellular
R17853 T23826 T23825 arg1Of analysis,FACS
R17854 T23826 T23827 arg1Of analysis,of
R17855 T23826 T23830 arg1Of analysis,in
R17856 T23826 T23838 arg1Of analysis,(
R17857 T23826 T23847 arg1Of analysis,","
R17858 T23826 T23848 arg2Of analysis,rested
R17859 T23826 T23850 arg2Of analysis,activated
R17860 T23829 T23827 arg2Of expression,of
R17861 T23829 T23828 arg1Of expression,FOXP3
R17862 T23831 T23832 arg1Of Th1,","
R17863 T23832 T23835 arg1Of ",",or
R17864 T23833 T23832 arg2Of Th2,","
R17865 T23835 T23834 arg1Of or,","
R17866 T23836 T23835 arg2Of iTreg-differentiated,or
R17867 T23837 T23830 arg2Of cells,in
R17868 T23837 T23831 arg1Of cells,Th1
R17869 T23837 T23833 arg1Of cells,Th2
R17870 T23837 T23836 arg1Of cells,iTreg-differentiated
R17871 T23840 T23839 arg1Of rounds,two
R17872 T23840 T23841 arg1Of rounds,","
R17873 T23840 T23843 arg1Of rounds,see
R17874 T23842 T23841 arg2Of phenotype,","
R17875 T23843 T23838 arg2Of see,(
R17876 T23845 T23843 arg2Of S1,see
R17877 T23845 T23844 arg1Of S1,Figure
R17878 T23846 T23838 arg3Of ),(
R17879 T23848 T23849 arg1Of rested,or
R17880 T23849 T23851 arg1Of or,","
R17881 T23849 T23852 arg1Of or,with
R17882 T23849 T23854 arg1Of or,without
R17883 T23850 T23849 arg2Of activated,or
R17884 T23852 T23853 arg1Of with,or
R17885 T23854 T23853 arg2Of without,or
R17886 T23855 T23852 arg2Of TGF-β,with
R17887 T23855 T23854 arg2Of TGF-β,without
R17888 T23857 T23856 arg1Of expression,FOXP3
R17889 T23857 T23858 arg1Of expression,was
R17890 T23857 T23859 arg2Of expression,measured
R17891 T23859 T23858 arg2Of measured,was
R17892 T23859 T23860 arg1Of measured,after
R17893 T23862 T23860 arg2Of d,after
R17894 T23862 T23861 arg1Of d,5
R17895 T23862 T23863 arg1Of d,in
R17896 T23864 T23863 arg2Of culture,in
R17897 T23867 T23865 arg1Of blots,The
R17898 T23867 T23866 arg1Of blots,dot
R17899 T23867 T23868 arg1Of blots,are
R17900 T23867 T23869 arg1Of blots,representative
R17901 T23869 T23868 arg2Of representative,are
R17902 T23869 T23870 arg1Of representative,of
R17903 T23873 T23870 arg2Of experiments,of
R17904 T23873 T23871 arg1Of experiments,three
R17905 T23873 T23872 arg1Of experiments,independent
R17906 T23875 T23874 arg2Of B,(
R17907 T23875 T23877 arg1Of B,Shows
R17908 T23876 T23874 arg3Of ),(
R17909 T23877 T23883 arg1Of Shows,but
R17910 T23881 T23877 arg2Of setup,Shows
R17911 T23881 T23878 arg1Of setup,the
R17912 T23881 T23879 arg1Of setup,same
R17913 T23881 T23880 arg1Of setup,experimental
R17914 T23883 T23882 arg1Of but,","
R17915 T23885 T23884 arg1Of occurring,naturally
R17916 T23887 T23885 arg1Of cells,occurring
R17917 T23887 T23886 arg1Of cells,Th2
R17918 T23887 T23888 arg1Of cells,were
R17919 T23887 T23889 arg2Of cells,analyzed
R17920 T23889 T23883 arg2Of analyzed,but
R17921 T23889 T23888 arg2Of analyzed,were
R17922 T23890 T23891 arg1Of Data,are
R17923 T23890 T23892 arg1Of Data,representative
R17924 T23892 T23891 arg2Of representative,are
R17925 T23892 T23893 arg1Of representative,of
R17926 T23896 T23893 arg2Of experiments,of
R17927 T23896 T23894 arg1Of experiments,three
R17928 T23896 T23895 arg1Of experiments,independent
R18062 T24133 T24134 arg1Of Th2-,or
R18063 T24138 T24134 arg2Of FOXP3,or
R18064 T24138 T24135 arg1Of FOXP3,IL-4–Producing
R18065 T24138 T24136 arg1Of FOXP3,Cells
R18066 T24138 T24137 arg1Of FOXP3,Lack
R18067 T24140 T24139 arg2Of A,(
R18068 T24141 T24139 arg3Of ),(
R18069 T24143 T24139 arg1Of analysis,(
R18070 T24143 T24142 arg1Of analysis,FACS
R18071 T24143 T24144 arg1Of analysis,of
R18072 T24143 T24150 arg1Of analysis,following
R18073 T24146 T24147 arg1Of FOXP3,and
R18074 T24148 T24147 arg2Of IL-4,and
R18075 T24149 T24144 arg2Of expression,of
R18076 T24149 T24145 arg1Of expression,intracellular
R18077 T24149 T24146 arg1Of expression,FOXP3
R18078 T24149 T24148 arg1Of expression,IL-4
R18079 T24152 T24150 arg2Of stimulation,following
R18080 T24152 T24151 arg1Of stimulation,PMA/Ionomycin
R18081 T24155 T24153 arg1Of cells,CD4+
R18082 T24155 T24154 arg1Of cells,T
R18083 T24155 T24156 arg1Of cells,were
R18084 T24155 T24157 arg2Of cells,gated
R18085 T24157 T24156 arg2Of gated,were
R18086 T24157 T24158 arg1Of gated,on
R18087 T24157 T24172 arg1Of gated,and
R18088 T24160 T24158 arg2Of basis,on
R18089 T24160 T24159 arg1Of basis,the
R18090 T24160 T24161 arg1Of basis,of
R18091 T24162 T24163 arg1Of CD45RO,and
R18092 T24164 T24163 arg2Of CD25,and
R18093 T24166 T24161 arg2Of expression,of
R18094 T24166 T24162 arg1Of expression,CD45RO
R18095 T24166 T24164 arg1Of expression,CD25
R18096 T24166 T24165 arg1Of expression,surface
R18097 T24166 T24167 arg1Of expression,(
R18098 T24169 T24167 arg2Of panel,(
R18099 T24169 T24168 arg1Of panel,upper
R18100 T24170 T24167 arg3Of ),(
R18101 T24172 T24171 arg1Of and,","
R18102 T24174 T24173 arg2Of cells,gated
R18103 T24174 T24175 arg1Of cells,are
R18104 T24174 T24176 arg2Of cells,shown
R18105 T24176 T24172 arg2Of shown,and
R18106 T24176 T24175 arg2Of shown,are
R18107 T24176 T24177 arg1Of shown,below
R18108 T24176 T24178 arg1Of shown,for
R18109 T24180 T24179 arg1Of CD45RO+CD25−,the
R18110 T24180 T24181 arg1Of CD45RO+CD25−,(
R18111 T24180 T24187 arg1Of CD45RO+CD25−,","
R18112 T24180 T24195 arg1Of CD45RO+CD25−,and
R18113 T24182 T24181 arg2Of A,(
R18114 T24182 T24183 arg1Of A,","
R18115 T24185 T24183 arg2Of panel,","
R18116 T24185 T24184 arg1Of panel,left
R18117 T24186 T24181 arg3Of ),(
R18118 T24189 T24187 arg2Of CD45RO+CD25+,","
R18119 T24189 T24188 arg1Of CD45RO+CD25+,the
R18120 T24189 T24190 arg1Of CD45RO+CD25+,(
R18121 T24192 T24190 arg2Of panel,(
R18122 T24192 T24191 arg1Of panel,right
R18123 T24193 T24190 arg3Of ),(
R18124 T24195 T24178 arg2Of and,for
R18125 T24195 T24194 arg1Of and,","
R18126 T24198 T24195 arg2Of subsets,and
R18127 T24198 T24196 arg1Of subsets,the
R18128 T24198 T24197 arg1Of subsets,CD45RO−CD25−
R18129 T24198 T24199 arg1Of subsets,(
R18130 T24201 T24199 arg2Of panel,(
R18131 T24201 T24200 arg1Of panel,central
R18132 T24202 T24199 arg3Of ),(
R18133 T24205 T24203 arg1Of analysis,A
R18134 T24205 T24204 arg1Of analysis,statistical
R18135 T24205 T24206 arg1Of analysis,of
R18136 T24205 T24210 arg1Of analysis,after
R18137 T24205 T24216 arg1Of analysis,are
R18138 T24205 T24217 arg2Of analysis,shown
R18139 T24209 T24206 arg2Of donors,of
R18140 T24209 T24207 arg1Of donors,eight
R18141 T24209 T24208 arg1Of donors,independent
R18142 T24211 T24210 arg2Of subtraction,after
R18143 T24211 T24212 arg1Of subtraction,of
R18144 T24215 T24212 arg2Of control,of
R18145 T24215 T24213 arg1Of control,the
R18146 T24215 T24214 arg1Of control,isotype
R18147 T24217 T24216 arg2Of shown,are
R18148 T24217 T24218 arg1Of shown,in
R18149 T24220 T24218 arg2Of B,in
R18150 T24220 T24219 arg2Of B,(
R18151 T24221 T24219 arg3Of ),(
R18152 T24225 T24222 arg1Of line,The
R18153 T24225 T24223 arg2Of line,dotted
R18154 T24225 T24224 arg1Of line,gray
R18155 T24225 T24226 arg1Of line,indicates
R18156 T24230 T24226 arg2Of level,indicates
R18157 T24230 T24227 arg1Of level,the
R18158 T24230 T24228 arg1Of level,IC
R18159 T24230 T24229 arg1Of level,background
R18160 T24233 T24231 arg1Of bars,The
R18161 T24233 T24232 arg1Of bars,error
R18162 T24233 T24234 arg1Of bars,show
R18163 T24234 T24243 arg1Of show,Similarly
R18164 T24234 T24244 arg1Of show,","
R18165 T24234 T24259 arg1Of show,and
R18166 T24236 T24234 arg2Of error,show
R18167 T24236 T24235 arg1Of error,the
R18168 T24236 T24237 arg1Of error,of
R18169 T24239 T24237 arg2Of mean.,of
R18170 T24239 T24238 arg1Of mean.,the
R18171 T24239 T24240 arg1Of mean.,(
R18172 T24241 T24240 arg2Of C,(
R18173 T24242 T24240 arg3Of ),(
R18174 T24247 T24234 arg3Of clone,show
R18175 T24247 T24245 arg1Of clone,a
R18176 T24247 T24246 arg1Of clone,Th2
R18177 T24247 T24248 arg1Of clone,(
R18178 T24247 T24251 arg1Of clone,","
R18179 T24247 T24255 arg1Of clone,","
R18180 T24249 T24248 arg2Of BR8,(
R18181 T24250 T24248 arg3Of ),(
R18182 T24254 T24251 arg2Of cells,","
R18183 T24254 T24252 arg1Of cells,CRTH2+
R18184 T24254 T24253 arg1Of cells,Th2
R18185 T24257 T24255 arg2Of cells,","
R18186 T24257 T24256 arg1Of cells,IL-4-secreting
R18187 T24259 T24258 arg1Of and,","
R18188 T24262 T24260 arg1Of cells,memory
R18189 T24262 T24261 arg1Of cells,T
R18190 T24262 T24263 arg1Of cells,(
R18191 T24262 T24266 arg1Of cells,were
R18192 T24262 T24267 arg2Of cells,stained
R18193 T24264 T24263 arg2Of CD45RO,(
R18194 T24265 T24263 arg3Of ),(
R18195 T24267 T24259 arg2Of stained,and
R18196 T24267 T24266 arg2Of stained,were
R18197 T24267 T24268 arg1Of stained,for
R18198 T24269 T24270 arg1Of FOXP3,and
R18199 T24270 T24268 arg2Of and,for
R18200 T24271 T24270 arg2Of IL-4,and
R18201 T24272 T24273 arg1Of Data,are
R18202 T24272 T24274 arg1Of Data,representative
R18203 T24274 T24273 arg2Of representative,are
R18204 T24274 T24275 arg1Of representative,of
R18205 T24278 T24275 arg2Of experiments,of
R18206 T24278 T24276 arg1Of experiments,three
R18207 T24278 T24277 arg1Of experiments,independent
R18443 T24707 T24706 arg1Of Induction,FOXP3
R18444 T24707 T24708 arg1Of Induction,During
R18445 T24711 T24708 arg2Of Process,During
R18446 T24711 T24709 arg1Of Process,the
R18447 T24711 T24710 arg1Of Process,Differentiation
R18448 T24713 T24712 arg2Of A,(
R18449 T24714 T24712 arg3Of ),(
R18450 T24718 T24712 arg1Of cells,(
R18451 T24718 T24715 arg1Of cells,Human
R18452 T24718 T24716 arg1Of cells,CD4+CD45RA+
R18453 T24718 T24717 arg1Of cells,T
R18454 T24718 T24719 arg1Of cells,were
R18455 T24718 T24720 arg2Of cells,activated
R18456 T24720 T24719 arg2Of activated,were
R18457 T24720 T24721 arg1Of activated,with
R18458 T24720 T24724 arg1Of activated,in
R18459 T24723 T24721 arg2Of anti-CD3/CD28,with
R18460 T24723 T24722 arg1Of anti-CD3/CD28,plate-bound
R18461 T24724 T24725 arg1Of in,the
R18462 T24724 T24727 arg1Of in,of
R18463 T24726 T24724 arg2Of presence,in
R18464 T24728 T24729 arg1Of TGF-β,(
R18465 T24728 T24733 arg1Of TGF-β,or
R18466 T24731 T24729 arg2Of ng/ml,(
R18467 T24731 T24730 arg1Of ng/ml,5
R18468 T24732 T24729 arg3Of ),(
R18469 T24733 T24724 arg3Of or,in
R18470 T24734 T24733 arg2Of IL-4,or
R18471 T24734 T24735 arg1Of IL-4,(
R18472 T24737 T24735 arg2Of ng/ml,(
R18473 T24737 T24736 arg1Of ng/ml,25
R18474 T24738 T24735 arg3Of ),(
R18475 T24740 T24739 arg1Of cells,The
R18476 T24740 T24741 arg1Of cells,were
R18477 T24740 T24742 arg2Of cells,harvested
R18478 T24742 T24741 arg2Of harvested,were
R18479 T24742 T24743 arg1Of harvested,at
R18480 T24742 T24748 arg1Of harvested,and
R18481 T24746 T24743 arg2Of points,at
R18482 T24746 T24744 arg1Of points,different
R18483 T24746 T24745 arg1Of points,time
R18484 T24748 T24747 arg1Of and,","
R18485 T24749 T24750 arg1Of mRNA,was
R18486 T24749 T24751 arg2Of mRNA,quantified
R18487 T24751 T24748 arg2Of quantified,and
R18488 T24751 T24750 arg2Of quantified,was
R18489 T24754 T24751 arg1Of PCR,quantified
R18490 T24754 T24752 arg2Of PCR,by
R18491 T24754 T24753 arg1Of PCR,real-time
R18492 T24754 T24755 arg1Of PCR,for
R18493 T24756 T24757 arg1Of FOXP3,and
R18494 T24758 T24757 arg2Of GATA3,and
R18495 T24759 T24755 arg2Of expression,for
R18496 T24759 T24756 arg1Of expression,FOXP3
R18497 T24759 T24758 arg1Of expression,GATA3
R18498 T24760 T24761 arg1Of Bars,show
R18499 T24765 T24761 arg2Of SD,show
R18500 T24765 T24762 arg1Of SD,the
R18501 T24765 T24763 arg1Of SD,mean
R18502 T24765 T24764 arg1Of SD,±
R18503 T24765 T24766 arg1Of SD,of
R18504 T24769 T24766 arg2Of experiments,of
R18505 T24769 T24767 arg1Of experiments,three
R18506 T24769 T24768 arg1Of experiments,independent
R18507 T24771 T24770 arg2Of B,(
R18508 T24772 T24770 arg3Of ),(
R18509 T24774 T24775 arg1Of GATA3,and
R18510 T24776 T24775 arg2Of FOXP3,and
R18511 T24777 T24770 arg1Of staining,(
R18512 T24777 T24773 arg1Of staining,Intracellular
R18513 T24777 T24774 arg1Of staining,GATA3
R18514 T24777 T24776 arg1Of staining,FOXP3
R18515 T24777 T24778 arg1Of staining,is
R18516 T24777 T24779 arg2Of staining,shown
R18517 T24779 T24778 arg2Of shown,is
R18518 T24779 T24780 arg1Of shown,after
R18519 T24781 T24780 arg2Of exposure,after
R18520 T24781 T24782 arg1Of exposure,of
R18521 T24781 T24786 arg1Of exposure,to
R18522 T24785 T24782 arg2Of cells,of
R18523 T24785 T24783 arg1Of cells,CD4+CD45RA+
R18524 T24785 T24784 arg1Of cells,T
R18525 T24788 T24786 arg2Of conditions,to
R18526 T24788 T24787 arg1Of conditions,differentiating
R18527 T24788 T24790 arg1Of conditions,in
R18528 T24790 T24789 arg1Of in,as
R18529 T24792 T24790 arg2Of A,in
R18530 T24792 T24791 arg1Of A,part
R18531 T24792 T24793 arg1Of A,of
R18532 T24795 T24793 arg2Of figure,of
R18533 T24795 T24794 arg1Of figure,the
R18534 T24796 T24797 arg1Of Data,are
R18535 T24796 T24798 arg1Of Data,representative
R18536 T24798 T24797 arg2Of representative,are
R18537 T24798 T24799 arg1Of representative,of
R18538 T24802 T24799 arg2Of experiments,of
R18539 T24802 T24800 arg1Of experiments,three
R18540 T24802 T24801 arg1Of experiments,independent
R18709 T25158 T25159 arg1Of Effect,of
R18710 T25158 T25161 arg1Of Effect,on
R18711 T25160 T25159 arg2Of IL-4,of
R18712 T25163 T25161 arg2Of Induction,on
R18713 T25163 T25162 arg1Of Induction,FOXP3
R18714 T25165 T25164 arg2Of A,(
R18715 T25166 T25164 arg3Of ),(
R18716 T25169 T25164 arg1Of analysis,(
R18717 T25169 T25167 arg1Of analysis,A
R18718 T25169 T25168 arg1Of analysis,statistical
R18719 T25169 T25170 arg1Of analysis,was
R18720 T25169 T25171 arg2Of analysis,performed
R18721 T25171 T25170 arg2Of performed,was
R18722 T25171 T25172 arg1Of performed,with
R18723 T25171 T25185 arg1Of performed,with
R18724 T25171 T25187 arg1Of performed,without
R18725 T25171 T25194 arg1Of performed,;
R18726 T25172 T25184 arg1Of with,and
R18727 T25174 T25172 arg2Of donors,with
R18728 T25174 T25173 arg1Of donors,six
R18729 T25174 T25175 arg1Of donors,on
R18730 T25179 T25175 arg2Of TGF-β,on
R18731 T25179 T25176 arg1Of TGF-β,day
R18732 T25179 T25177 arg1Of TGF-β,5
R18733 T25179 T25178 arg1Of TGF-β,(
R18734 T25179 T25180 arg1Of TGF-β,(
R18735 T25182 T25180 arg2Of ng/ml,(
R18736 T25182 T25181 arg1Of ng/ml,10
R18737 T25183 T25180 arg3Of ),(
R18738 T25185 T25186 arg1Of with,or
R18739 T25186 T25184 arg2Of or,and
R18740 T25187 T25186 arg2Of without,or
R18741 T25188 T25185 arg2Of IL-4,with
R18742 T25188 T25187 arg2Of IL-4,without
R18743 T25188 T25189 arg1Of IL-4,(
R18744 T25188 T25193 arg1Of IL-4,)
R18745 T25191 T25189 arg2Of ng/ml,(
R18746 T25191 T25190 arg1Of ng/ml,100
R18747 T25192 T25189 arg3Of ),(
R18748 T25195 T25196 arg1Of Shown,is
R18749 T25196 T25200 arg1Of is,and
R18750 T25198 T25196 arg2Of mean,is
R18751 T25198 T25197 arg1Of mean,the
R18752 T25200 T25194 arg2Of and,;
R18753 T25200 T25199 arg1Of and,","
R18754 T25202 T25201 arg1Of bars,error
R18755 T25202 T25203 arg1Of bars,indicated
R18756 T25203 T25200 arg2Of indicated,and
R18757 T25205 T25203 arg2Of SD,indicated
R18758 T25205 T25204 arg1Of SD,the
R18759 T25205 T25206 arg1Of SD,of
R18760 T25208 T25206 arg2Of donors,of
R18761 T25208 T25207 arg1Of donors,six
R18762 T25210 T25209 arg1Of analysis,Statistical
R18763 T25210 T25211 arg1Of analysis,was
R18764 T25210 T25212 arg2Of analysis,performed
R18765 T25212 T25211 arg2Of performed,was
R18766 T25213 T25212 arg3Of using,performed
R18767 T25216 T25213 arg2Of test,using
R18768 T25216 T25214 arg1Of test,the
R18769 T25216 T25215 arg1Of test,Dunnett
R18770 T25218 T25217 arg1Of significance,Statistical
R18771 T25218 T25219 arg1Of significance,is
R18772 T25218 T25220 arg2Of significance,indicated
R18773 T25220 T25219 arg2Of indicated,is
R18774 T25222 T25220 arg1Of asterisks,indicated
R18775 T25222 T25221 arg2Of asterisks,by
R18776 T25222 T25223 arg1Of asterisks,(
R18777 T25225 T25223 arg2Of ≤,(
R18778 T25225 T25224 arg1Of ≤,*p
R18779 T25225 T25226 arg1Of ≤,0.05
R18780 T25225 T25227 arg1Of ≤,","
R18781 T25228 T25227 arg2Of **p,","
R18782 T25228 T25230 arg1Of **p,0.01
R18783 T25228 T25231 arg1Of **p,","
R18784 T25230 T25229 arg1Of 0.01,≤
R18785 T25232 T25231 arg2Of Dunnett,","
R18786 T25233 T25223 arg3Of ),(
R18787 T25235 T25234 arg2Of B,(
R18788 T25236 T25234 arg3Of ),(
R18789 T25238 T25234 arg1Of cells,(
R18790 T25238 T25237 arg1Of cells,CD4+CD45RA+
R18791 T25238 T25239 arg1Of cells,were
R18792 T25238 T25240 arg2Of cells,activated
R18793 T25240 T25239 arg2Of activated,were
R18794 T25240 T25241 arg1Of activated,in
R18795 T25240 T25260 arg1Of activated,","
R18796 T25240 T25261 arg1Of activated,as
R18797 T25241 T25242 arg1Of in,the
R18798 T25241 T25244 arg1Of in,of
R18799 T25243 T25241 arg2Of presence,in
R18800 T25247 T25241 arg3Of concentration,in
R18801 T25247 T25245 arg1Of concentration,a
R18802 T25247 T25246 arg1Of concentration,constant
R18803 T25247 T25248 arg1Of concentration,of
R18804 T25247 T25254 arg1Of concentration,with
R18805 T25249 T25248 arg2Of TGF-β,of
R18806 T25249 T25250 arg1Of TGF-β,(
R18807 T25252 T25250 arg2Of ng/ml,(
R18808 T25252 T25251 arg1Of ng/ml,5
R18809 T25253 T25250 arg3Of ),(
R18810 T25257 T25254 arg2Of concentration,with
R18811 T25257 T25255 arg1Of concentration,an
R18812 T25257 T25256 arg1Of concentration,increasing
R18813 T25257 T25258 arg1Of concentration,of
R18814 T25259 T25258 arg2Of IL-4,of
R18815 T25262 T25261 arg2Of indicated,as
R18816 T25263 T25264 arg1Of Cells,were
R18817 T25263 T25265 arg2Of Cells,harvested
R18818 T25265 T25264 arg2Of harvested,were
R18819 T25265 T25266 arg1Of harvested,for
R18820 T25265 T25269 arg1Of harvested,after
R18821 T25268 T25266 arg2Of quantification,for
R18822 T25268 T25267 arg1Of quantification,mRNA
R18823 T25271 T25269 arg2Of d,after
R18824 T25271 T25270 arg1Of d,5
R18825 T25273 T25272 arg2Of C,(
R18826 T25274 T25272 arg3Of ),(
R18827 T25276 T25272 arg1Of cells,(
R18828 T25276 T25275 arg1Of cells,CD4+CD45RA+
R18829 T25276 T25277 arg1Of cells,were
R18830 T25276 T25278 arg2Of cells,stimulated
R18831 T25278 T25277 arg2Of stimulated,were
R18832 T25278 T25280 arg1Of stimulated,vitro
R18833 T25278 T25281 arg1Of stimulated,with
R18834 T25278 T25296 arg1Of stimulated,as
R18835 T25280 T25279 arg1Of vitro,in
R18836 T25283 T25282 arg1Of anti-CD3/CD28,plate-bound
R18837 T25283 T25284 arg1Of anti-CD3/CD28,","
R18838 T25284 T25290 arg1Of ",",and
R18839 T25285 T25284 arg2Of TGF-β,","
R18840 T25285 T25286 arg1Of TGF-β,(
R18841 T25287 T25286 arg2Of 10ng/ml,(
R18842 T25288 T25286 arg3Of ),(
R18843 T25290 T25281 arg2Of and,with
R18844 T25290 T25289 arg1Of and,","
R18845 T25291 T25290 arg2Of IL-4,and
R18846 T25291 T25292 arg1Of IL-4,(
R18847 T25294 T25292 arg2Of ng/ml,(
R18848 T25294 T25293 arg1Of ng/ml,100
R18849 T25295 T25292 arg3Of ),(
R18850 T25297 T25296 arg2Of indicated,as
R18851 T25300 T25298 arg2Of h,After
R18852 T25300 T25299 arg1Of h,1
R18853 T25303 T25302 arg1Of lysates,cell
R18854 T25303 T25304 arg1Of lysates,were
R18855 T25303 T25305 arg2Of lysates,prepared
R18856 T25303 T25307 arg2Of lysates,analyzed
R18857 T25305 T25306 arg1Of prepared,and
R18858 T25306 T25298 arg1Of and,After
R18859 T25306 T25301 arg1Of and,","
R18860 T25306 T25304 arg2Of and,were
R18861 T25307 T25306 arg2Of analyzed,and
R18862 T25310 T25305 arg1Of blot,prepared
R18863 T25310 T25307 arg1Of blot,analyzed
R18864 T25310 T25308 arg2Of blot,by
R18865 T25310 T25309 arg1Of blot,Western
R18866 T25310 T25311 arg1Of blot,for
R18867 T25313 T25314 arg1Of SMAD2,and
R18868 T25314 T25311 arg2Of and,for
R18869 T25314 T25312 arg2Of and,phosphorylated
R18870 T25315 T25314 arg2Of STAT6,and
R18871 T25317 T25318 arg1Of STAT6,and
R18872 T25318 T25316 arg1Of and,Total
R18873 T25318 T25320 arg1Of and,served
R18874 T25319 T25318 arg2Of GAPDH,and
R18875 T25320 T25321 arg1Of served,as
R18876 T25323 T25321 arg2Of control,as
R18877 T25323 T25322 arg1Of control,internal
R18878 T25325 T25324 arg2Of D,(
R18879 T25326 T25324 arg3Of ),(
R18880 T25328 T25329 arg1Of GATA3,and
R18881 T25330 T25329 arg2Of FOXP3,and
R18882 T25331 T25324 arg1Of staining,(
R18883 T25331 T25327 arg1Of staining,Intracellular
R18884 T25331 T25328 arg1Of staining,GATA3
R18885 T25331 T25330 arg1Of staining,FOXP3
R18886 T25331 T25332 arg1Of staining,are
R18887 T25331 T25333 arg2Of staining,shown
R18888 T25333 T25332 arg2Of shown,are
R18889 T25333 T25334 arg1Of shown,after
R18890 T25335 T25334 arg2Of exposure,after
R18891 T25335 T25336 arg1Of exposure,of
R18892 T25339 T25336 arg2Of cells,of
R18893 T25339 T25337 arg1Of cells,CD4+CD45RA+
R18894 T25339 T25338 arg1Of cells,T
R18895 T25341 T25340 arg1Of IL-4,to
R18896 T25341 T25342 arg1Of IL-4,as
R18897 T25341 T25348 arg1Of IL-4,are
R18898 T25341 T25349 arg1Of IL-4,representative
R18899 T25343 T25342 arg2Of described,as
R18900 T25343 T25344 arg1Of described,for
R18901 T25347 T25344 arg2Of Data,for
R18902 T25347 T25345 arg1Of Data,panel
R18903 T25347 T25346 arg1Of Data,B.
R18904 T25348 T25332 modOf are,are
R18905 T25349 T25348 arg2Of representative,are
R18906 T25349 T25350 arg1Of representative,of
R18907 T25353 T25350 arg2Of experiments,of
R18908 T25353 T25351 arg1Of experiments,three
R18909 T25353 T25352 arg1Of experiments,independent
R19189 T25880 T25881 arg1Of IL-4,Inhibits
R19190 T25884 T25881 arg2Of Commitment,Inhibits
R19191 T25884 T25882 arg1Of Commitment,TGF-β–Mediated
R19192 T25884 T25883 arg1Of Commitment,iTreg
R19193 T25887 T25885 arg1Of cells,CFSE-labeled
R19194 T25887 T25886 arg1Of cells,CD4+CD45RA+
R19195 T25887 T25888 arg1Of cells,were
R19196 T25887 T25889 arg2Of cells,activated
R19197 T25889 T25888 arg2Of activated,were
R19198 T25889 T25890 arg1Of activated,with
R19199 T25889 T25898 arg1Of activated,","
R19200 T25889 T25899 arg1Of activated,as
R19201 T25892 T25891 arg1Of anti-CD3/CD28,plate-bound
R19202 T25892 T25893 arg1Of anti-CD3/CD28,","
R19203 T25893 T25896 arg1Of ",",and
R19204 T25894 T25893 arg2Of TGF-β,","
R19205 T25896 T25890 arg2Of and,with
R19206 T25896 T25895 arg1Of and,","
R19207 T25897 T25896 arg2Of IL-4,and
R19208 T25900 T25899 arg2Of indicated,as
R19209 T25903 T25901 arg2Of d,After
R19210 T25903 T25902 arg1Of d,5
R19211 T25905 T25906 arg1Of cells,were
R19212 T25905 T25907 arg2Of cells,analyzed
R19213 T25907 T25906 arg2Of analyzed,were
R19214 T25907 T25908 arg1Of analyzed,by
R19215 T25907 T25914 arg1Of analyzed,and
R19216 T25910 T25908 arg2Of cytometry,by
R19217 T25910 T25909 arg1Of cytometry,flow
R19218 T25910 T25911 arg1Of cytometry,(
R19219 T25912 T25911 arg2Of A,(
R19220 T25913 T25911 arg3Of ),(
R19221 T25914 T25901 arg1Of and,After
R19222 T25914 T25904 arg1Of and,","
R19223 T25915 T25916 arg1Of results,of
R19224 T25915 T25920 arg1Of results,are
R19225 T25915 T25921 arg2Of results,shown
R19226 T25919 T25916 arg2Of experiments,of
R19227 T25919 T25917 arg1Of experiments,six
R19228 T25919 T25918 arg1Of experiments,independent
R19229 T25921 T25914 arg2Of shown,and
R19230 T25921 T25920 arg2Of shown,are
R19231 T25921 T25922 arg1Of shown,in
R19232 T25925 T25922 arg2Of graph,in
R19233 T25925 T25923 arg1Of graph,the
R19234 T25925 T25924 arg1Of graph,bar
R19235 T25925 T25926 arg1Of graph,below
R19236 T25925 T25927 arg1Of graph,(
R19237 T25928 T25927 arg2Of B,(
R19238 T25929 T25927 arg3Of ),(
R19239 T25931 T25930 arg1Of significance,Statistical
R19240 T25931 T25932 arg1Of significance,(
R19241 T25931 T25938 arg1Of significance,is
R19242 T25931 T25939 arg2Of significance,indicated
R19243 T25934 T25932 arg2Of Anova,(
R19244 T25934 T25933 arg1Of Anova,one-way
R19245 T25934 T25935 arg1Of Anova,","
R19246 T25934 T25936 arg1Of Anova,Newman-Keuls
R19247 T25937 T25932 arg3Of ),(
R19248 T25939 T25938 arg2Of indicated,is
R19249 T25941 T25939 arg1Of asterisks,indicated
R19250 T25941 T25940 arg2Of asterisks,by
R19251 T25941 T25942 arg1Of asterisks,(
R19252 T25941 T25962 modOf asterisks,st
R19253 T25941 T25973 arg1Of asterisks,CD
R19254 T25943 T25942 arg2Of **p,(
R19255 T25943 T25944 arg1Of **p,≤
R19256 T25943 T25945 arg1Of **p,0.01
R19257 T25943 T25946 arg1Of **p,","
R19258 T25949 T25946 arg2Of 0.001,","
R19259 T25949 T25947 arg1Of 0.001,***p
R19260 T25949 T25948 arg1Of 0.001,≤
R19261 T25950 T25942 arg3Of ),(
R19262 T25952 T25951 arg2Of (,.
R19263 T25953 T25951 arg3Of C,.
R19264 T25955 T25951 arg1Of ic analy,.
R19265 T25955 T25954 arg1Of ic analy,) Kinet
R19266 T25955 T25956 arg1Of ic analy,si
R19267 T25955 T25962 arg1Of ic analy,st
R19268 T25955 T25963 arg2Of ic analy,ainin
R19269 T25958 T25959 arg1Of lular,GAT
R19270 T25960 T25959 arg2Of A3 an,GAT
R19271 T25961 T25956 arg2Of d FOXP3 ,si
R19272 T25961 T25957 arg1Of d FOXP3 ,s of intracel
R19273 T25961 T25958 arg1Of d FOXP3 ,lular
R19274 T25961 T25960 arg1Of d FOXP3 ,A3 an
R19275 T25963 T25962 arg2Of ainin,st
R19276 T25963 T25964 arg1Of ainin,g
R19277 T25963 T25967 arg1Of ainin,wn in pan
R19278 T25966 T25964 arg2Of o,g
R19279 T25966 T25965 arg1Of o,is sh
R19280 T25968 T25967 arg2Of el C fol,wn in pan
R19281 T25968 T25969 arg1Of el C fol,lo
R19282 T25972 T25969 arg2Of e of ,lo
R19283 T25972 T25970 arg1Of e of ,wing exposu
R19284 T25972 T25971 arg1Of e of ,r
R19285 T25976 T25977 arg1Of ells,to
R19286 T25977 T25973 arg2Of to ,CD
R19287 T25977 T25974 arg1Of to ,4+CD45RA+ T
R19288 T25977 T25975 arg1Of to ,c
R19289 T25978 T25977 arg2Of anti-,to
R19290 T25979 T25980 arg1Of Data,are
R19291 T25979 T25981 arg1Of Data,representative
R19292 T25981 T25980 arg2Of representative,are
R19293 T25981 T25982 arg1Of representative,of
R19294 T25985 T25982 arg2Of experiments,of
R19295 T25985 T25983 arg1Of experiments,three
R19296 T25985 T25984 arg1Of experiments,independent
R19451 T26347 T26348 arg1Of GATA3,Acts
R19452 T26348 T26349 arg1Of Acts,as
R19453 T26352 T26349 arg2Of Regulator,as
R19454 T26352 T26350 arg1Of Regulator,a
R19455 T26352 T26351 arg1Of Regulator,Negative
R19456 T26352 T26353 arg1Of Regulator,of
R19457 T26355 T26353 arg2Of Expression,of
R19458 T26355 T26354 arg1Of Expression,FOXP3
R19459 T26357 T26356 arg2Of A,(
R19460 T26358 T26356 arg3Of ),(
R19461 T26363 T26356 arg1Of cells,(
R19462 T26363 T26359 arg1Of cells,Human
R19463 T26363 T26360 arg1Of cells,naive
R19464 T26363 T26361 arg1Of cells,CD4+CDRA+
R19465 T26363 T26362 arg1Of cells,T
R19466 T26363 T26364 arg1Of cells,were
R19467 T26363 T26365 arg2Of cells,transduced
R19468 T26365 T26364 arg2Of transduced,were
R19469 T26365 T26366 arg1Of transduced,with
R19470 T26365 T26380 arg1Of transduced,and
R19471 T26367 T26368 arg1Of 0,","
R19472 T26368 T26370 arg1Of ",",","
R19473 T26369 T26368 arg2Of 20,","
R19474 T26370 T26373 arg1Of ",",and
R19475 T26371 T26370 arg2Of 100,","
R19476 T26373 T26366 arg2Of and,with
R19477 T26373 T26372 arg1Of and,","
R19478 T26375 T26373 arg2Of nM,and
R19479 T26375 T26374 arg1Of nM,500
R19480 T26375 T26376 arg1Of nM,of
R19481 T26378 T26376 arg2Of protein,of
R19482 T26378 T26377 arg1Of protein,TAT-GATA3
R19483 T26380 T26379 arg1Of and,","
R19484 T26382 T26381 arg1Of presence,intracellular
R19485 T26382 T26383 arg1Of presence,of
R19486 T26382 T26385 arg1Of presence,was
R19487 T26382 T26386 arg2Of presence,analyzed
R19488 T26384 T26383 arg2Of GATA3,of
R19489 T26386 T26380 arg2Of analyzed,and
R19490 T26386 T26385 arg2Of analyzed,was
R19491 T26387 T26386 arg3Of using,analyzed
R19492 T26387 T26389 arg1Of using,following
R19493 T26388 T26387 arg2Of FACS,using
R19494 T26391 T26389 arg2Of activation,following
R19495 T26391 T26390 arg1Of activation,anti-CD3/CD28
R19496 T26391 T26392 arg1Of activation,of
R19497 T26394 T26392 arg2Of cells,of
R19498 T26394 T26393 arg1Of cells,the
R19499 T26396 T26395 arg1Of cells,GFP-positive
R19500 T26396 T26397 arg1Of cells,were
R19501 T26396 T26398 arg2Of cells,gated
R19502 T26396 T26400 arg2Of cells,analyzed
R19503 T26398 T26399 arg1Of gated,and
R19504 T26399 T26397 arg2Of and,were
R19505 T26400 T26399 arg2Of analyzed,and
R19506 T26400 T26401 arg1Of analyzed,for
R19507 T26400 T26405 arg1Of analyzed,following
R19508 T26404 T26401 arg2Of expression,for
R19509 T26404 T26402 arg1Of expression,intracellular
R19510 T26404 T26403 arg1Of expression,FOXP3
R19511 T26409 T26405 arg2Of period,following
R19512 T26409 T26406 arg1Of period,a
R19513 T26409 T26407 arg1Of period,2-d
R19514 T26409 T26408 arg1Of period,incubation
R19515 T26409 T26410 arg1Of period,(
R19516 T26412 T26410 arg2Of panel,(
R19517 T26412 T26411 arg1Of panel,lower
R19518 T26413 T26410 arg3Of ),(
R19519 T26414 T26415 arg1Of Data,are
R19520 T26414 T26416 arg1Of Data,representative
R19521 T26416 T26415 arg2Of representative,are
R19522 T26416 T26417 arg1Of representative,of
R19523 T26420 T26417 arg2Of experiments,of
R19524 T26420 T26418 arg1Of experiments,four
R19525 T26420 T26419 arg1Of experiments,independent
R19526 T26422 T26421 arg2Of B,(
R19527 T26423 T26421 arg3Of ),(
R19528 T26426 T26421 arg1Of cells,(
R19529 T26426 T26424 arg1Of cells,CD4+CD25−
R19530 T26426 T26425 arg1Of cells,T
R19531 T26426 T26427 arg1Of cells,were
R19532 T26426 T26428 arg2Of cells,isolated
R19533 T26426 T26435 arg2Of cells,treated
R19534 T26428 T26429 arg1Of isolated,from
R19535 T26428 T26434 arg1Of isolated,and
R19536 T26430 T26431 arg1Of D011.10,and
R19537 T26432 T26431 arg2Of D011.10xCD2-GATA3,and
R19538 T26433 T26429 arg2Of mice,from
R19539 T26433 T26430 arg1Of mice,D011.10
R19540 T26433 T26432 arg1Of mice,D011.10xCD2-GATA3
R19541 T26434 T26427 arg2Of and,were
R19542 T26435 T26434 arg2Of treated,and
R19543 T26435 T26436 arg1Of treated,with
R19544 T26435 T26440 arg1Of treated,for
R19545 T26437 T26438 arg1Of OVA,and
R19546 T26438 T26436 arg2Of and,with
R19547 T26439 T26438 arg2Of TGF-β,and
R19548 T26442 T26440 arg2Of h,for
R19549 T26442 T26441 arg1Of h,96
R19550 T26444 T26443 arg1Of CD4,Surface
R19551 T26444 T26445 arg1Of CD4,and
R19552 T26445 T26448 arg1Of and,were
R19553 T26445 T26449 arg2Of and,measured
R19554 T26447 T26445 arg2Of FOXP3,and
R19555 T26447 T26446 arg1Of FOXP3,intracellular
R19556 T26449 T26448 arg2Of measured,were
R19557 T26451 T26449 arg1Of FACS,measured
R19558 T26451 T26450 arg2Of FACS,by
R19559 T26453 T26452 arg1Of data,These
R19560 T26453 T26454 arg1Of data,are
R19561 T26453 T26455 arg1Of data,representative
R19562 T26455 T26454 arg2Of representative,are
R19563 T26455 T26456 arg1Of representative,of
R19564 T26459 T26456 arg2Of experiments,of
R19565 T26459 T26457 arg1Of experiments,three
R19566 T26459 T26458 arg1Of experiments,independent
R19567 T26461 T26462 arg1Of C,)
R19568 T26464 T26461 arg1Of cells,C
R19569 T26464 T26463 arg1Of cells,The
R19570 T26464 T26465 arg2Of cells,treated
R19571 T26464 T26471 arg1Of cells,were
R19572 T26464 T26472 arg2Of cells,harvested
R19573 T26465 T26467 arg1Of treated,in
R19574 T26467 T26466 arg1Of in,as
R19575 T26469 T26467 arg2Of B,in
R19576 T26469 T26468 arg2Of B,(
R19577 T26470 T26468 arg3Of ),(
R19578 T26472 T26471 arg2Of harvested,were
R19579 T26472 T26473 arg1Of harvested,and
R19580 T26473 T26460 arg1Of and,(
R19581 T26474 T26475 arg1Of mRNA,was
R19582 T26474 T26476 arg2Of mRNA,quantified
R19583 T26476 T26473 arg2Of quantified,and
R19584 T26476 T26475 arg2Of quantified,was
R19585 T26479 T26476 arg1Of PCR,quantified
R19586 T26479 T26477 arg2Of PCR,by
R19587 T26479 T26478 arg1Of PCR,real-time
R19588 T26479 T26480 arg1Of PCR,for
R19589 T26481 T26482 arg1Of SMAD7,and
R19590 T26483 T26482 arg2Of TGF-β,and
R19591 T26484 T26480 arg2Of expression,for
R19592 T26484 T26481 arg1Of expression,SMAD7
R19593 T26484 T26483 arg1Of expression,TGF-β
R19594 T26485 T26486 arg1Of Bars,show
R19595 T26490 T26486 arg2Of SD,show
R19596 T26490 T26487 arg1Of SD,the
R19597 T26490 T26488 arg1Of SD,mean
R19598 T26490 T26489 arg1Of SD,±
R19599 T26490 T26491 arg1Of SD,of
R19600 T26494 T26491 arg2Of experiments,of
R19601 T26494 T26492 arg1Of experiments,three
R19602 T26494 T26493 arg1Of experiments,independent
R19818 T26944 T26945 arg1Of GATA3,Represses
R19822 T26949 T26945 arg2Of Promoter,Represses
R19827 T26954 T26958 arg1Of ",",and
R19829 T26956 T26955 arg1Of cells,Th2
R19830 T26958 T26957 arg1Of and,","
R19831 T26958 T26963 arg1Of and,were
R19832 T26958 T26964 arg2Of and,transfected
R19833 T26962 T26958 arg2Of cells,and
R19834 T26962 T26959 arg1Of cells,human
R19835 T26962 T26960 arg1Of cells,primary
R19836 T26962 T26961 arg1Of cells,CD4
R19837 T26964 T26963 arg2Of transfected,were
R19838 T26964 T26965 arg1Of transfected,with
R19839 T26971 T26970 arg1Of basic,pGL3
R19840 T26974 T26972 arg1Of a,)
R19841 T26974 T26973 arg1Of a,or
R19842 T26975 T26965 arg2Of vector,with
R19843 T26975 T26966 arg1Of vector,an
R19844 T26975 T26967 arg1Of vector,empty
R19845 T26975 T26968 arg1Of vector,vector
R19846 T26975 T26969 arg1Of vector,(
R19847 T26975 T26971 arg1Of vector,basic
R19848 T26975 T26974 arg1Of vector,a
R19849 T26975 T26976 arg1Of vector,containing
R19850 T26981 T26976 arg2Of region,containing
R19851 T26981 T26977 arg1Of region,the
R19852 T26981 T26978 arg1Of region,putative
R19853 T26981 T26979 arg1Of region,FOXP3
R19854 T26981 T26980 arg1Of region,promoter
R19855 T26981 T26982 arg2Of region,fused
R19856 T26982 T26983 arg1Of fused,to
R19857 T26987 T26983 arg2Of gene,to
R19858 T26987 T26984 arg1Of gene,the
R19859 T26987 T26985 arg1Of gene,luciferase
R19860 T26987 T26986 arg1Of gene,reporter
R19861 T26988 T26989 arg1Of Bars,show
R19862 T26989 T27006 arg1Of show,;
R19863 T26993 T26989 arg2Of SD,show
R19864 T26993 T26990 arg1Of SD,the
R19865 T26993 T26991 arg1Of SD,mean
R19866 T26993 T26992 arg1Of SD,±
R19867 T26993 T26994 arg1Of SD,of
R19868 T26997 T26994 arg2Of units,of
R19869 T26997 T26995 arg1Of units,arbitrary
R19870 T26997 T26996 arg1Of units,light
R19871 T26997 T26998 arg2Of units,normalized
R19872 T26998 T26999 arg1Of normalized,for
R19873 T27001 T26999 arg2Of luciferase,for
R19874 T27001 T27000 arg1Of luciferase,renilla
R19875 T27001 T27002 arg1Of luciferase,of
R19876 T27005 T27002 arg2Of experiments,of
R19877 T27005 T27003 arg1Of experiments,four
R19878 T27005 T27004 arg1Of experiments,independent
R19879 T27007 T27008 arg1Of samples,were
R19880 T27007 T27009 arg2Of samples,measured
R19881 T27009 T27006 arg2Of measured,;
R19882 T27009 T27008 arg2Of measured,were
R19883 T27009 T27010 arg1Of measured,in
R19884 T27011 T27010 arg2Of triplicates,in
R19885 T27013 T27012 arg2Of B,(
R19886 T27014 T27012 arg3Of ),(
R19887 T27018 T27012 arg1Of cells,(
R19888 T27018 T27015 arg1Of cells,Naive
R19889 T27018 T27016 arg1Of cells,CD4
R19890 T27018 T27017 arg1Of cells,T
R19891 T27018 T27019 arg1Of cells,were
R19892 T27018 T27020 arg2Of cells,transfected
R19893 T27020 T27019 arg2Of transfected,were
R19894 T27020 T27021 arg1Of transfected,with
R19895 T27026 T27022 arg1Of construct,the
R19896 T27026 T27023 arg1Of construct,FOXP3
R19897 T27026 T27024 arg1Of construct,promoter
R19898 T27026 T27025 arg1Of construct,reporter
R19899 T27026 T27028 arg1Of construct,with
R19900 T27028 T27021 arg2Of with,with
R19901 T27028 T27027 arg1Of with,together
R19902 T27032 T27029 arg1Of vector,a
R19903 T27032 T27030 arg1Of vector,GATA3
R19904 T27032 T27031 arg1Of vector,expression
R19905 T27032 T27033 arg1Of vector,or
R19906 T27033 T27028 arg2Of or,with
R19907 T27036 T27033 arg2Of vector,or
R19908 T27036 T27034 arg1Of vector,an
R19909 T27036 T27035 arg1Of vector,empty
R19910 T27037 T27038 arg1Of Bars,show
R19911 T27042 T27038 arg2Of SD,show
R19912 T27042 T27039 arg1Of SD,the
R19913 T27042 T27040 arg1Of SD,mean
R19914 T27042 T27041 arg1Of SD,±
R19915 T27042 T27043 arg1Of SD,of
R19916 T27046 T27043 arg2Of experiments,of
R19917 T27046 T27044 arg1Of experiments,three
R19918 T27046 T27045 arg1Of experiments,independent
R19919 T27048 T27047 arg2Of C,(
R19920 T27049 T27047 arg3Of ),(
R19921 T27053 T27047 arg1Of panel,(
R19922 T27053 T27050 arg1Of panel,Naive
R19923 T27053 T27051 arg2Of panel,(
R19924 T27053 T27052 arg1Of panel,left
R19925 T27053 T27055 arg1Of panel,or
R19926 T27054 T27051 arg3Of ),(
R19927 T27056 T27055 arg2Of memory,or
R19928 T27056 T27057 arg1Of memory,(
R19929 T27059 T27057 arg2Of panel,(
R19930 T27059 T27058 arg1Of panel,right
R19931 T27060 T27057 arg3Of ),(
R19932 T27063 T27051 arg1Of cells,(
R19933 T27063 T27056 arg1Of cells,memory
R19934 T27063 T27061 arg1Of cells,CD4
R19935 T27063 T27062 arg1Of cells,T
R19936 T27063 T27064 arg1Of cells,were
R19937 T27063 T27065 arg2Of cells,transfected
R19938 T27063 T27077 arg2Of cells,activated
R19939 T27065 T27066 arg1Of transfected,with
R19940 T27065 T27076 arg1Of transfected,and
R19941 T27067 T27068 arg1Of wild-type,or
R19942 T27068 T27066 arg2Of or,with
R19943 T27071 T27070 arg1Of mutated,GATA3
R19944 T27075 T27068 arg2Of construct,or
R19945 T27075 T27069 arg1Of construct,a
R19946 T27075 T27071 arg1Of construct,mutated
R19947 T27075 T27072 arg1Of construct,511-FOXP3
R19948 T27075 T27073 arg1Of construct,promoter
R19949 T27075 T27074 arg1Of construct,reporter
R19950 T27076 T27064 arg2Of and,were
R19951 T27077 T27076 arg2Of activated,and
R19952 T27077 T27078 arg1Of activated,with
R19953 T27079 T27080 arg1Of PMA,and
R19954 T27080 T27078 arg2Of and,with
R19955 T27081 T27080 arg2Of ionomycin,and
R19956 T27082 T27083 arg1Of Bars,show
R19957 T27083 T27100 arg1Of show,;
R19958 T27087 T27083 arg2Of SD,show
R19959 T27087 T27084 arg1Of SD,the
R19960 T27087 T27085 arg1Of SD,mean
R19961 T27087 T27086 arg1Of SD,±
R19962 T27087 T27088 arg1Of SD,of
R19963 T27091 T27088 arg2Of units,of
R19964 T27091 T27089 arg1Of units,arbitrary
R19965 T27091 T27090 arg1Of units,light
R19966 T27091 T27092 arg2Of units,normalized
R19967 T27092 T27093 arg1Of normalized,for
R19968 T27095 T27093 arg2Of luciferase,for
R19969 T27095 T27094 arg1Of luciferase,renilla
R19970 T27095 T27096 arg1Of luciferase,of
R19971 T27099 T27096 arg2Of experiments,of
R19972 T27099 T27097 arg1Of experiments,eight
R19973 T27099 T27098 arg1Of experiments,independent
R19974 T27101 T27102 arg1Of samples,were
R19975 T27101 T27103 arg2Of samples,measured
R19976 T27103 T27100 arg2Of measured,;
R19977 T27103 T27102 arg2Of measured,were
R19978 T27103 T27104 arg1Of measured,in
R19979 T27105 T27104 arg2Of triplicates,in
R19980 T27107 T27106 arg2Of D,(
R19981 T27108 T27106 arg3Of ),(
R19982 T27110 T27106 arg1Of extracts,(
R19983 T27110 T27109 arg1Of extracts,Nuclear
R19984 T27110 T27111 arg1Of extracts,were
R19985 T27110 T27112 arg2Of extracts,prepared
R19986 T27112 T27111 arg2Of prepared,were
R19987 T27112 T27113 arg1Of prepared,from
R19988 T27115 T27113 arg2Of cells,from
R19989 T27115 T27114 arg1Of cells,HEK
R19990 T27115 T27116 arg2Of cells,transfected
R19991 T27116 T27117 arg1Of transfected,with
R19992 T27118 T27119 arg1Of GATA3,or
R19993 T27119 T27117 arg2Of or,with
R19994 T27122 T27119 arg2Of vector,or
R19995 T27122 T27120 arg1Of vector,an
R19996 T27122 T27121 arg1Of vector,empty
R19997 T27125 T27124 arg2Of E,(
R19998 T27126 T27124 arg3Of ),(
R19999 T27127 T27128 arg1Of Th1,","
R20000 T27128 T27131 arg1Of ",",or
R20001 T27129 T27128 arg2Of Th2,","
R20002 T27131 T27130 arg1Of or,","
R20003 T27132 T27131 arg2Of iTreg,or
R20004 T27133 T27134 arg1Of cells,and
R20005 T27134 T27124 arg1Of and,(
R20006 T27134 T27127 arg1Of and,Th1
R20007 T27134 T27129 arg1Of and,Th2
R20008 T27134 T27132 arg1Of and,iTreg
R20009 T27134 T27137 arg1Of and,precipitated
R20010 T27136 T27134 arg2Of factors,and
R20011 T27136 T27135 arg1Of factors,binding
R20012 T27137 T27111 modOf precipitated,were
R20013 T27137 T27123 arg1Of precipitated,","
R20014 T27138 T27137 arg2Of using,precipitated
R20015 T27140 T27138 arg2Of oligonucleotides,using
R20016 T27140 T27139 arg2Of oligonucleotides,biotinylated
R20017 T27144 T27141 arg1Of complexes,The
R20018 T27144 T27142 arg1Of complexes,oligonucleotides–transcription
R20019 T27144 T27143 arg1Of complexes,factor
R20020 T27144 T27145 arg1Of complexes,were
R20021 T27144 T27146 arg2Of complexes,separated
R20022 T27146 T27145 arg2Of separated,were
R20023 T27146 T27147 arg1Of separated,on
R20024 T27150 T27147 arg2Of gel,on
R20025 T27150 T27148 arg1Of gel,a
R20026 T27150 T27149 arg1Of gel,SDS-PAGE
R20027 T27152 T27151 arg1Of amounts,The
R20028 T27152 T27153 arg1Of amounts,of
R20029 T27152 T27156 arg1Of amounts,in
R20030 T27152 T27159 arg1Of amounts,were
R20031 T27152 T27160 arg2Of amounts,assessed
R20032 T27155 T27153 arg2Of protein,of
R20033 T27155 T27154 arg1Of protein,GATA3
R20034 T27158 T27156 arg2Of precipitates,in
R20035 T27158 T27157 arg1Of precipitates,the
R20036 T27160 T27159 arg2Of assessed,were
R20037 T27162 T27160 arg1Of immunoblotting,assessed
R20038 T27162 T27161 arg2Of immunoblotting,by
R20039 T27162 T27163 arg1Of immunoblotting,with
R20040 T27165 T27163 arg2Of mAb,with
R20041 T27165 T27164 arg1Of mAb,anti-GATA3
R20042 T27168 T27166 arg1Of extracts,Total
R20043 T27168 T27167 arg1Of extracts,nuclear
R20044 T27168 T27169 arg1Of extracts,were
R20045 T27168 T27171 arg2Of extracts,run
R20046 T27171 T27169 arg2Of run,were
R20047 T27171 T27170 arg1Of run,also
R20048 T27171 T27172 arg1Of run,as
R20049 T27173 T27172 arg2Of controls,as
R20050 T27174 T27175 arg1Of Data,are
R20051 T27174 T27176 arg1Of Data,representative
R20052 T27176 T27175 arg2Of representative,are
R20053 T27176 T27177 arg1Of representative,of
R20054 T27180 T27177 arg2Of experiments,of
R20055 T27180 T27178 arg1Of experiments,three
R20056 T27180 T27179 arg1Of experiments,different
R20057 T27182 T27181 arg2Of F,(
R20058 T27183 T27181 arg3Of ),(
R20059 T27184 T27185 arg1Of iTreg,or
R20060 T27186 T27185 arg2Of Th2,or
R20061 T27187 T27181 arg1Of cells,(
R20062 T27187 T27184 arg1Of cells,iTreg
R20063 T27187 T27186 arg1Of cells,Th2
R20064 T27187 T27188 arg1Of cells,were
R20065 T27187 T27189 arg2Of cells,analyzed
R20066 T27189 T27188 arg2Of analyzed,were
R20067 T27191 T27189 arg1Of ChIP,analyzed
R20068 T27191 T27190 arg2Of ChIP,by
R20069 T27191 T27192 arg1Of ChIP,for
R20070 T27194 T27192 arg2Of binding,for
R20071 T27194 T27193 arg1Of binding,GATA3
R20072 T27194 T27195 arg1Of binding,to
R20073 T27198 T27195 arg2Of promoter,to
R20074 T27198 T27196 arg1Of promoter,the
R20075 T27198 T27197 arg1Of promoter,FOXP3
R20076 T27200 T27199 arg1Of “input”,The
R20077 T27200 T27201 arg1Of “input”,represents
R20078 T27203 T27201 arg2Of amplification,represents
R20079 T27203 T27202 arg1Of amplification,PCR
R20080 T27203 T27204 arg1Of amplification,of
R20081 T27207 T27204 arg2Of sample,of
R20082 T27207 T27205 arg1Of sample,the
R20083 T27207 T27206 arg1Of sample,total
R20084 T27207 T27208 arg1Of sample,","
R20085 T27207 T27209 arg1Of sample,which
R20086 T27207 T27210 arg1Of sample,was
R20087 T27207 T27212 arg2Of sample,subjected
R20088 T27212 T27210 arg2Of subjected,was
R20089 T27212 T27211 arg1Of subjected,not
R20090 T27212 T27213 arg1Of subjected,to
R20091 T27215 T27213 arg2Of precipitation,to
R20092 T27215 T27214 arg1Of precipitation,any
R20093 T27216 T27217 arg1Of Results,are
R20094 T27216 T27218 arg1Of Results,representative
R20095 T27218 T27217 arg2Of representative,are
R20096 T27218 T27219 arg1Of representative,of
R20097 T27222 T27219 arg2Of experiments,of
R20098 T27222 T27220 arg1Of experiments,three
R20099 T27222 T27221 arg1Of experiments,independent
R13422 T17783 T17784 arg1Of purchased,from
R13424 T17787 T17784 arg2Of Laboratories,from
R13425 T17787 T17785 arg1Of Laboratories,the
R13426 T17787 T17786 arg1Of Laboratories,Jackson
R13427 T17787 T17788 arg1Of Laboratories,(
R19819 T26949 T26946 arg1Of Promoter,the
R19820 T26949 T26947 arg1Of Promoter,Human
R19821 T26949 T26948 arg1Of Promoter,FOXP3
R19823 T26951 T26950 arg2Of A,(
R19824 T26952 T26950 arg3Of ),(
R19825 T26953 T26950 arg1Of Jurkat,(
R19826 T26953 T26954 arg1Of Jurkat,","
R19828 T26956 T26954 arg2Of cells,","
R14624 T19522 T19523 arg1Of Cloning,of
R14625 T19522 T19527 arg1Of Cloning,and
R14626 T19526 T19523 arg2Of promoter,of
R14627 T19526 T19524 arg1Of promoter,the
R14628 T19526 T19525 arg1Of promoter,FOXP3
R14629 T19528 T19527 arg2Of construction,and
R14630 T19528 T19529 arg1Of construction,of
R14631 T19531 T19529 arg2Of constructs,of
R14632 T19531 T19530 arg1Of constructs,mutant
R14633 T19534 T19532 arg1Of promoter,The
R14634 T19534 T19533 arg1Of promoter,FOXP3
R14635 T19534 T19535 arg1Of promoter,was
R15011 T20052 T20051 arg1Of isolation,RNA
R15012 T20052 T20053 arg1Of isolation,and
R15013 T20055 T20053 arg2Of synthesis,and
R15014 T20055 T20054 arg1Of synthesis,cDNA
R15015 T20056 T20057 arg1Of RNA,was
R15016 T20056 T20058 arg2Of RNA,isolated

bionlp-st-ge-2016-coref

Id Subject Object Predicate Lexical cue
T335 541-544 Anaphor denotes its
T336 388-393 Antecedent denotes FOXP3
T337 1005-1020 Anaphor denotes This inhibition
T338 931-940 Antecedent denotes deficient
T1581 6197-6206 Anaphor denotes this gene
T1582 6144-6149 Antecedent denotes FOXP3
T12013 23765-23774 Anaphor denotes This site
T12014 23674-23691 Antecedent denotes GATA-binding site
T12015 24313-24326 Anaphor denotes the construct
T12016 24172-24180 Antecedent denotes promoter
T14964 28560-28573 Anaphor denotes these factors
T14965 28481-28486 Antecedent denotes T-bet
T14966 28491-28496 Antecedent denotes GATA3
T14967 28970-28990 Anaphor denotes polarization factors
T14968 29005-29009 Antecedent denotes IL-4
T14969 29011-29016 Antecedent denotes IL-12
T14970 29021-29026 Antecedent denotes TGF-β
T14971 31170-31190 Anaphor denotes these concentrations
T14972 31095-31099 Antecedent denotes IL-4
T14973 34648-34657 Anaphor denotes cytokines
T14974 34666-34670 Antecedent denotes IL-4
T6958 16331-16355 Anaphor denotes the suppressive capacity
T6959 16288-16295 Antecedent denotes inhibit
T5528 12168-12173 Anaphor denotes their
T5529 12127-12132 Antecedent denotes GATA3
T5530 12137-12142 Antecedent denotes FOXP3
T8704 19805-19817 Anaphor denotes both factors
T8705 19709-19714 Antecedent denotes GATA3
T8706 19719-19724 Antecedent denotes FOXP3
R634 T335 T336 boundBy its,FOXP3
R635 T337 T338 boundBy This inhibition,deficient
R1373 T1581 T1582 boundBy this gene,FOXP3
R9040 T12013 T12014 boundBy This site,GATA-binding site
R9041 T12015 T12016 boundBy the construct,promoter
R11393 T14964 T14965 boundBy these factors,T-bet
R11394 T14964 T14966 boundBy these factors,GATA3
R11395 T14967 T14968 boundBy polarization factors,IL-4
R11396 T14967 T14969 boundBy polarization factors,IL-12
R11397 T14967 T14970 boundBy polarization factors,TGF-β
R11398 T14971 T14972 boundBy these concentrations,IL-4
R11399 T14973 T14974 boundBy cytokines,IL-4
R5400 T6958 T6959 boundBy the suppressive capacity,inhibit
R4312 T5528 T5529 boundBy their,GATA3
R4313 T5528 T5530 boundBy their,FOXP3
R6681 T8704 T8705 boundBy both factors,GATA3
R6682 T8704 T8706 boundBy both factors,FOXP3

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T361 0-12 CD denotes GATA3-Driven
T362 13-16 NNP denotes Th2
T363 17-26 NNS denotes Responses
T364 27-34 VBP denotes Inhibit
T365 35-41 NN denotes TGF-β1
T366 42-49 NNP denotes Induced
T367 50-55 NNP denotes FOXP3
T368 56-66 NNP denotes Expression
T369 67-70 CC denotes and
T370 71-74 DT denotes the
T371 75-84 NN denotes Formation
T372 85-87 IN denotes of
T373 88-98 NNP denotes Regulatory
T374 99-100 NNP denotes T
T375 101-106 NNP denotes Cells
T376 159-172 NNP denotes Transcription
T377 173-180 NNS denotes factors
T378 181-184 VBP denotes act
T379 185-187 IN denotes in
T380 188-195 NN denotes concert
T381 196-198 TO denotes to
T382 199-205 VB denotes induce
T383 206-213 NN denotes lineage
T384 214-224 NN denotes commitment
T385 225-232 IN denotes towards
T386 233-236 NNP denotes Th1
T387 236-237 , denotes ,
T388 238-241 NNP denotes Th2
T389 241-242 , denotes ,
T390 243-245 CC denotes or
T391 246-247 NNP denotes T
T392 248-258 JJ denotes regulatory
T393 259-260 -LRB- denotes (
T394 260-264 NNP denotes Treg
T395 264-265 -RRB- denotes )
T396 266-271 NNS denotes cells
T397 271-272 , denotes ,
T398 273-276 CC denotes and
T399 277-282 PRP$ denotes their
T400 283-301 JJ denotes counter-regulatory
T401 302-312 NNS denotes mechanisms
T402 313-317 VBD denotes were
T403 318-323 VBN denotes shown
T404 324-326 TO denotes to
T405 327-329 VB denotes be
T406 330-338 JJ denotes critical
T407 339-342 IN denotes for
T408 343-355 NN denotes polarization
T409 356-363 IN denotes between
T410 364-367 CD denotes Th1
T411 368-371 CC denotes and
T412 372-375 CD denotes Th2
T413 376-386 NNS denotes phenotypes
T414 386-387 . denotes .
T415 388-393 CD denotes FOXP3
T416 394-396 VBZ denotes is
T417 397-399 DT denotes an
T418 400-409 JJ denotes essential
T419 410-423 NN denotes transcription
T420 424-430 NN denotes factor
T421 431-434 IN denotes for
T422 435-442 JJ denotes natural
T423 442-443 , denotes ,
T424 444-458 JJ denotes thymus-derived
T425 459-460 -LRB- denotes (
T426 460-465 NNP denotes nTreg
T427 465-466 -RRB- denotes )
T428 467-470 CC denotes and
T429 471-480 JJ denotes inducible
T430 481-485 NNP denotes Treg
T431 486-487 -LRB- denotes (
T432 487-492 NNP denotes iTreg
T433 492-493 -RRB- denotes )
T434 494-504 NN denotes commitment
T435 504-505 : denotes ;
T436 506-513 RB denotes however
T437 513-514 , denotes ,
T438 515-518 DT denotes the
T439 519-529 NNS denotes mechanisms
T440 530-540 VBG denotes regulating
T441 541-544 PRP$ denotes its
T442 545-555 NN denotes expression
T443 556-559 VBP denotes are
T444 560-562 RB denotes as
T445 563-566 RB denotes yet
T446 567-574 JJ denotes unknown
T447 574-575 . denotes .
T448 576-578 PRP denotes We
T449 579-587 VBP denotes describe
T450 588-589 DT denotes a
T451 590-599 NN denotes mechanism
T452 600-611 VBG denotes controlling
T453 612-617 NNP denotes iTreg
T454 618-630 NN denotes polarization
T455 630-631 , denotes ,
T456 632-637 WDT denotes which
T457 638-640 VBZ denotes is
T458 641-650 VBN denotes overruled
T459 651-653 IN denotes by
T460 654-657 DT denotes the
T461 658-661 JJ denotes Th2
T462 662-677 NN denotes differentiation
T463 678-685 NN denotes pathway
T464 685-686 . denotes .
T465 687-689 PRP denotes We
T466 690-702 VBD denotes demonstrated
T467 703-707 IN denotes that
T468 708-719 NN denotes interleukin
T469 720-721 CD denotes 4
T470 722-723 -LRB- denotes (
T471 723-727 NN denotes IL-4
T472 727-728 -RRB- denotes )
T473 729-736 NN denotes present
T474 737-739 IN denotes at
T475 740-743 DT denotes the
T476 744-748 NN denotes time
T477 749-751 IN denotes of
T478 752-753 NNP denotes T
T479 754-758 NN denotes cell
T480 759-766 VBG denotes priming
T481 767-775 NNS denotes inhibits
T482 776-781 NNP denotes FOXP3
T483 781-782 . denotes .
T484 783-787 DT denotes This
T485 788-798 JJ denotes inhibitory
T486 799-808 NN denotes mechanism
T487 809-812 VBD denotes was
T488 813-817 RB denotes also
T489 818-827 VBN denotes confirmed
T490 828-830 IN denotes in
T491 831-834 JJ denotes Th2
T492 835-840 NNS denotes cells
T493 841-844 CC denotes and
T494 845-847 IN denotes in
T495 848-849 NNP denotes T
T496 850-855 NNS denotes cells
T497 856-858 IN denotes of
T498 859-869 JJ denotes transgenic
T499 870-874 NNS denotes mice
T500 875-889 VBG denotes overexpressing
T501 890-896 NN denotes GATA-3
T502 897-899 IN denotes in
T503 900-901 NNP denotes T
T504 902-907 NNS denotes cells
T505 907-908 , denotes ,
T506 909-914 WDT denotes which
T507 915-918 VBP denotes are
T508 919-924 VBN denotes shown
T509 925-927 TO denotes to
T510 928-930 VB denotes be
T511 931-940 JJ denotes deficient
T512 941-943 IN denotes in
T513 944-956 VBG denotes transforming
T514 957-963 NN denotes growth
T515 964-970 NN denotes factor
T516 971-972 -LRB- denotes (
T517 972-975 NNP denotes TGF
T518 975-976 -RRB- denotes )
T519 976-977 : denotes -
T520 977-978 NN denotes β
T521 979-987 VBD denotes mediated
T522 988-993 CD denotes FOXP3
T523 994-1003 NN denotes induction
T524 1003-1004 . denotes .
T525 1005-1009 DT denotes This
T526 1010-1020 NN denotes inhibition
T527 1021-1023 VBZ denotes is
T528 1024-1032 VBN denotes mediated
T529 1033-1035 IN denotes by
T530 1036-1042 JJ denotes direct
T531 1043-1050 JJ denotes binding
T532 1051-1053 IN denotes of
T533 1054-1059 CD denotes GATA3
T534 1060-1062 TO denotes to
T535 1063-1066 DT denotes the
T536 1067-1072 CD denotes FOXP3
T537 1073-1081 NN denotes promoter
T538 1081-1082 , denotes ,
T539 1083-1088 WDT denotes which
T540 1089-1098 VBZ denotes represses
T541 1099-1102 PRP$ denotes its
T542 1103-1118 NN denotes transactivation
T543 1119-1126 NN denotes process
T544 1126-1127 . denotes .
T545 1128-1137 RB denotes Therefore
T546 1137-1138 , denotes ,
T547 1139-1143 DT denotes this
T548 1144-1149 NN denotes study
T549 1150-1158 VBZ denotes provides
T550 1159-1160 DT denotes a
T551 1161-1164 JJ denotes new
T552 1165-1178 NN denotes understanding
T553 1179-1181 IN denotes of
T554 1182-1191 NN denotes tolerance
T555 1192-1203 NN denotes development
T556 1203-1204 , denotes ,
T557 1205-1215 VBN denotes controlled
T558 1216-1218 IN denotes by
T559 1219-1220 DT denotes a
T560 1221-1225 NN denotes type
T561 1226-1227 CD denotes 2
T562 1228-1234 JJ denotes immune
T563 1235-1243 NN denotes response
T564 1243-1244 . denotes .
T565 1245-1249 NN denotes IL-4
T566 1250-1259 NN denotes treatment
T567 1260-1262 IN denotes in
T568 1263-1267 NNS denotes mice
T569 1268-1275 VBZ denotes reduces
T570 1276-1281 NNP denotes iTreg
T571 1282-1286 NN denotes cell
T572 1287-1296 NN denotes frequency
T573 1296-1297 , denotes ,
T574 1298-1310 VBG denotes highlighting
T575 1311-1315 IN denotes that
T576 1316-1327 JJ denotes therapeutic
T577 1328-1338 NNS denotes approaches
T578 1339-1343 WDT denotes that
T579 1344-1350 VBP denotes target
T580 1351-1355 NN denotes IL-4
T581 1356-1358 CC denotes or
T582 1359-1364 NN denotes GATA3
T583 1365-1370 MD denotes might
T584 1371-1378 VB denotes provide
T585 1379-1382 JJ denotes new
T586 1383-1393 JJ denotes preventive
T587 1394-1404 NNS denotes strategies
T588 1405-1417 VBG denotes facilitating
T589 1418-1427 NN denotes tolerance
T590 1428-1437 NN denotes induction
T591 1438-1450 RB denotes particularly
T592 1451-1453 IN denotes in
T593 1454-1466 JJ denotes Th2-mediated
T594 1467-1475 NNS denotes diseases
T595 1475-1476 , denotes ,
T596 1477-1481 JJ denotes such
T597 1482-1484 IN denotes as
T598 1485-1492 NN denotes allergy
T599 1492-1493 . denotes .
T1634 2849-2858 JJ denotes Effective
T1635 2859-2865 JJ denotes immune
T1636 2866-2875 NNS denotes responses
T1637 2876-2879 VBP denotes are
T1638 2880-2893 VBN denotes characterized
T1639 2894-2896 IN denotes by
T1640 2897-2898 NNP denotes T
T1641 2899-2903 NN denotes cell
T1642 2904-2914 NN denotes activation
T1643 2914-2915 , denotes ,
T1644 2916-2921 WDT denotes which
T1645 2922-2929 VBZ denotes directs
T1646 2930-2938 JJ denotes adaptive
T1647 2939-2942 CC denotes and
T1648 2943-2949 JJ denotes innate
T1649 2950-2956 JJ denotes immune
T1650 2957-2966 NNS denotes responses
T1651 2967-2969 TO denotes to
T1652 2970-2974 VB denotes kill
T1653 2975-2984 NNS denotes pathogens
T1654 2985-2996 RB denotes efficiently
T1655 2996-2997 . denotes .
T1656 2998-3007 JJ denotes Dependent
T1657 3008-3010 IN denotes on
T1658 3011-3014 DT denotes the
T1659 3015-3023 NN denotes pathogen
T1660 3023-3024 , denotes ,
T1661 3025-3026 NNP denotes T
T1662 3027-3032 NNS denotes cells
T1663 3033-3046 VBP denotes differentiate
T1664 3047-3051 IN denotes into
T1665 3052-3061 JJ denotes different
T1666 3062-3070 NNS denotes subtypes
T1667 3070-3071 , denotes ,
T1668 3072-3076 JJ denotes such
T1669 3077-3079 IN denotes as
T1670 3080-3083 CD denotes Th1
T1671 3084-3086 CC denotes or
T1672 3087-3090 CD denotes Th2
T1673 3091-3096 NNS denotes cells
T1674 3096-3097 , denotes ,
T1675 3098-3103 WDT denotes which
T1676 3104-3107 VBP denotes are
T1677 3108-3112 RBS denotes most
T1678 3113-3122 JJ denotes efficient
T1679 3123-3125 IN denotes in
T1680 3126-3135 VBG denotes defeating
T1681 3136-3145 JJ denotes microbial
T1682 3146-3148 CC denotes or
T1683 3149-3158 JJ denotes parasitic
T1684 3159-3167 NNS denotes invaders
T1685 3168-3180 RB denotes respectively
T1686 3180-3181 . denotes .
T1687 3182-3183 DT denotes A
T1688 3184-3192 NN denotes hallmark
T1689 3193-3195 IN denotes of
T1690 3196-3199 CD denotes Th1
T1691 3200-3203 CC denotes and
T1692 3204-3207 CD denotes Th2
T1693 3208-3223 NN denotes differentiation
T1694 3224-3232 NNS denotes pathways
T1695 3233-3235 VBZ denotes is
T1696 3236-3239 DT denotes the
T1697 3240-3253 NN denotes exclusiveness
T1698 3254-3256 IN denotes of
T1699 3257-3260 DT denotes the
T1700 3261-3271 JJ denotes individual
T1701 3272-3281 NN denotes phenotype
T1702 3282-3289 VBG denotes leading
T1703 3290-3292 TO denotes to
T1704 3293-3299 DT denotes either
T1705 3300-3303 CD denotes Th1
T1706 3304-3306 CC denotes or
T1707 3307-3310 CD denotes Th2
T1708 3310-3311 , denotes ,
T1709 3312-3315 CC denotes but
T1710 3316-3319 RB denotes not
T1711 3320-3322 TO denotes to
T1712 3323-3328 JJ denotes mixed
T1713 3329-3340 NNS denotes populations
T1714 3340-3341 . denotes .
T1715 3342-3345 DT denotes The
T1716 3346-3359 NN denotes exclusiveness
T1717 3360-3362 IN denotes of
T1718 3363-3367 DT denotes this
T1719 3368-3377 NN denotes mechanism
T1720 3378-3380 VBZ denotes is
T1721 3381-3389 VBN denotes provided
T1722 3390-3392 IN denotes by
T1723 3393-3394 DT denotes a
T1724 3395-3407 NN denotes polarization
T1725 3408-3415 NN denotes process
T1726 3415-3416 , denotes ,
T1727 3417-3422 WRB denotes where
T1728 3423-3426 JJ denotes Th2
T1729 3427-3442 NN denotes differentiation
T1730 3443-3451 VBZ denotes inhibits
T1731 3452-3455 JJ denotes Th1
T1732 3456-3466 NN denotes commitment
T1733 3467-3470 CC denotes and
T1734 3471-3475 NN denotes vice
T1735 3476-3481 RB denotes versa
T1736 3481-3482 . denotes .
T1737 3483-3495 RB denotes Specifically
T1738 3496-3507 VBG denotes interleukin
T1739 3508-3509 CD denotes 4
T1740 3510-3511 -LRB- denotes (
T1741 3511-3515 NN denotes IL-4
T1742 3515-3516 -RRB- denotes )
T1743 3517-3524 VBD denotes induced
T1744 3525-3530 NNP denotes STAT6
T1745 3531-3534 CC denotes and
T1746 3535-3540 NNP denotes GATA3
T1747 3541-3548 VBP denotes inhibit
T1748 3549-3564 NN denotes differentiation
T1749 3565-3569 IN denotes into
T1750 3570-3573 JJ denotes Th1
T1751 3574-3579 NNS denotes cells
T1752 3580-3582 IN denotes in
T1753 3583-3586 DT denotes the
T1754 3587-3592 JJ denotes early
T1755 3593-3598 NN denotes phase
T1756 3599-3601 IN denotes of
T1757 3602-3612 NN denotes commitment
T1758 3613-3614 NNP denotes [
T1759 3614-3617 CD denotes 1,2
T1760 3617-3618 NNP denotes ]
T1761 3618-3619 . denotes .
T1762 3620-3625 CD denotes GATA3
T1763 3626-3628 VBZ denotes is
T1764 3629-3639 JJ denotes sufficient
T1765 3640-3642 TO denotes to
T1766 3643-3649 VB denotes induce
T1767 3650-3651 DT denotes a
T1768 3652-3655 JJ denotes Th2
T1769 3656-3665 NN denotes phenotype
T1770 3666-3667 NN denotes [
T1771 3667-3668 CD denotes 3
T1772 3668-3669 NNP denotes ]
T1773 3670-3673 CC denotes and
T1774 3674-3678 VBZ denotes acts
T1775 3679-3682 RB denotes not
T1776 3683-3687 RB denotes only
T1777 3688-3695 IN denotes through
T1778 3696-3699 DT denotes the
T1779 3700-3709 NN denotes induction
T1780 3710-3712 IN denotes of
T1781 3713-3717 NN denotes IL-4
T1782 3717-3718 , denotes ,
T1783 3719-3723 NN denotes IL-5
T1784 3724-3727 CC denotes and
T1785 3728-3733 NN denotes IL-13
T1786 3733-3734 , denotes ,
T1787 3735-3738 DT denotes the
T1788 3739-3742 NNP denotes Th2
T1789 3743-3752 NNS denotes cytokines
T1790 3752-3753 , denotes ,
T1791 3754-3757 CC denotes but
T1792 3758-3762 RB denotes also
T1793 3763-3770 IN denotes through
T1794 3771-3774 DT denotes the
T1795 3775-3785 NN denotes inhibition
T1796 3786-3788 IN denotes of
T1797 3789-3792 CD denotes Th1
T1798 3793-3806 JJ denotes cell-specific
T1799 3807-3814 NNS denotes factors
T1800 3815-3816 VBP denotes [
T1801 3816-3817 CD denotes 3
T1802 3817-3818 NNP denotes ]
T1803 3818-3819 . denotes .
T1804 3820-3828 RB denotes Recently
T1805 3828-3829 , denotes ,
T1806 3830-3832 PRP denotes it
T1807 3833-3836 VBD denotes was
T1808 3837-3842 VBN denotes shown
T1809 3843-3847 IN denotes that
T1810 3848-3853 JJ denotes T-bet
T1811 3854-3862 RB denotes directly
T1812 3863-3872 VBZ denotes modulates
T1813 3873-3878 CD denotes GATA3
T1814 3879-3887 NN denotes function
T1815 3887-3888 , denotes ,
T1816 3889-3899 VBG denotes suggesting
T1817 3900-3904 IN denotes that
T1818 3905-3918 NN denotes transcription
T1819 3919-3926 NNS denotes factors
T1820 3927-3934 VBP denotes compete
T1821 3935-3937 IN denotes in
T1822 3938-3941 DT denotes the
T1823 3942-3947 JJ denotes early
T1824 3948-3963 NN denotes differentiation
T1825 3964-3969 NN denotes phase
T1826 3970-3972 IN denotes of
T1827 3973-3974 NNP denotes T
T1828 3975-3980 NNS denotes cells
T1829 3980-3981 , denotes ,
T1830 3982-3993 RB denotes potentially
T1831 3994-4005 VBG denotes integrating
T1832 4006-4019 JJ denotes environmental
T1833 4020-4027 NNS denotes signals
T1834 4028-4030 TO denotes to
T1835 4031-4038 RB denotes finally
T1836 4039-4046 VB denotes imprint
T1837 4047-4050 DT denotes the
T1838 4051-4052 NNP denotes T
T1839 4053-4057 NN denotes cell
T1840 4058-4067 NN denotes phenotype
T1841 4068-4069 NNP denotes [
T1842 4069-4072 CD denotes 4,5
T1843 4072-4073 NNP denotes ]
T1844 4073-4074 . denotes .
T1845 4075-4076 DT denotes A
T1846 4077-4092 JJ denotes GATA3-dominated
T1847 4093-4099 JJ denotes immune
T1848 4100-4108 NN denotes response
T1849 4109-4112 VBZ denotes has
T1850 4113-4117 VBN denotes been
T1851 4118-4123 VBN denotes shown
T1852 4124-4126 TO denotes to
T1853 4127-4129 VB denotes be
T1854 4130-4139 JJ denotes essential
T1855 4140-4142 IN denotes in
T1856 4143-4149 NN denotes airway
T1857 4150-4168 NN denotes hyperresonsiveness
T1858 4169-4170 NNP denotes [
T1859 4170-4171 CD denotes 6
T1860 4171-4172 NNP denotes ]
T1861 4173-4176 CC denotes and
T1862 4177-4181 NN denotes IL-4
T1863 4182-4191 VBD denotes dominated
T1864 4192-4201 NNS denotes responses
T1865 4202-4205 MD denotes can
T1866 4206-4211 VB denotes break
T1867 4212-4228 JJ denotes antigen-specific
T1868 4229-4235 JJ denotes immune
T1869 4236-4245 NN denotes tolerance
T1870 4246-4247 NN denotes [
T1871 4247-4248 CD denotes 7
T1872 4248-4249 NNP denotes ]
T1873 4249-4250 . denotes .
T1874 4251-4265 NNP denotes Overexpression
T1875 4266-4268 IN denotes of
T1876 4269-4270 DT denotes a
T1877 4271-4279 JJ denotes dominant
T1878 4280-4288 JJ denotes negative
T1879 4289-4293 NN denotes form
T1880 4294-4296 IN denotes of
T1881 4297-4302 NNP denotes GATA3
T1882 4303-4304 NNP denotes [
T1883 4304-4305 CD denotes 8
T1884 4305-4306 NNP denotes ]
T1885 4307-4309 CC denotes or
T1886 4310-4319 NN denotes treatment
T1887 4320-4324 IN denotes with
T1888 4325-4343 JJ denotes antisense-mediated
T1889 4344-4349 NNP denotes GATA3
T1890 4350-4358 NN denotes blockade
T1891 4359-4360 NN denotes [
T1892 4360-4361 CD denotes 9
T1893 4361-4362 NNP denotes ]
T1894 4363-4372 VBD denotes decreased
T1895 4373-4376 DT denotes the
T1896 4377-4385 NN denotes severity
T1897 4386-4388 IN denotes of
T1898 4389-4392 DT denotes the
T1899 4393-4401 JJ denotes allergic
T1900 4402-4408 NN denotes airway
T1901 4409-4429 NN denotes hyper-responsiveness
T1902 4429-4430 . denotes .
T1903 4431-4434 DT denotes The
T1904 4435-4444 NN denotes discovery
T1905 4445-4447 IN denotes of
T1906 4448-4458 JJ denotes regulatory
T1907 4459-4460 NNP denotes T
T1908 4461-4462 -LRB- denotes (
T1909 4462-4466 NNP denotes Treg
T1910 4466-4467 -RRB- denotes )
T1911 4468-4473 NNS denotes cells
T1912 4474-4484 VBZ denotes highlights
T1913 4485-4492 DT denotes another
T1914 4493-4502 NN denotes phenotype
T1915 4503-4505 IN denotes of
T1916 4506-4507 NNP denotes T
T1917 4508-4513 NNS denotes cells
T1918 4513-4514 , denotes ,
T1919 4515-4520 WDT denotes which
T1920 4521-4523 VBZ denotes is
T1921 4524-4533 JJ denotes essential
T1922 4534-4537 IN denotes for
T1923 4538-4547 NN denotes tolerance
T1924 4548-4555 IN denotes against
T1925 4556-4569 NNS denotes self-antigens
T1926 4569-4570 . denotes .
T1927 4571-4580 RB denotes Naturally
T1928 4581-4590 VBG denotes occurring
T1929 4590-4591 , denotes ,
T1930 4592-4606 JJ denotes thymus-derived
T1931 4607-4611 NNP denotes Treg
T1932 4612-4613 -LRB- denotes (
T1933 4613-4618 NNP denotes nTreg
T1934 4618-4619 -RRB- denotes )
T1935 4620-4625 NNS denotes cells
T1936 4626-4629 VBP denotes are
T1937 4630-4639 VBN denotes generated
T1938 4640-4642 IN denotes in
T1939 4643-4646 DT denotes the
T1940 4647-4653 NN denotes thymus
T1941 4654-4657 CC denotes and
T1942 4658-4661 VBP denotes are
T1943 4662-4669 VBN denotes assumed
T1944 4670-4672 TO denotes to
T1945 4673-4680 VB denotes protect
T1946 4681-4688 IN denotes against
T1947 4689-4692 DT denotes the
T1948 4693-4701 NN denotes activity
T1949 4702-4704 IN denotes of
T1950 4705-4717 JJ denotes autoreactive
T1951 4718-4719 NNP denotes T
T1952 4720-4725 NNS denotes cells
T1953 4726-4728 IN denotes in
T1954 4729-4732 DT denotes the
T1955 4733-4742 NN denotes periphery
T1956 4742-4743 . denotes .
T1957 4744-4749 DT denotes These
T1958 4750-4755 NNS denotes cells
T1959 4756-4763 VBP denotes express
T1960 4764-4767 DT denotes the
T1961 4768-4776 NN denotes forkhead
T1962 4777-4790 NN denotes transcription
T1963 4791-4797 NN denotes factor
T1964 4798-4803 CD denotes FOXP3
T1965 4804-4807 CC denotes and
T1966 4808-4822 RB denotes constitutively
T1967 4823-4830 JJ denotes express
T1968 4831-4835 NNS denotes CD25
T1969 4836-4838 IN denotes on
T1970 4839-4844 PRP$ denotes their
T1971 4845-4852 NN denotes surface
T1972 4852-4853 , denotes ,
T1973 4854-4857 CC denotes but
T1974 4858-4862 PRP denotes they
T1975 4863-4867 VBP denotes lack
T1976 4868-4878 NN denotes expression
T1977 4879-4881 IN denotes of
T1978 4882-4885 CD denotes Th1
T1979 4886-4888 CC denotes or
T1980 4889-4892 CD denotes Th2
T1981 4893-4902 NNS denotes cytokines
T1982 4902-4903 . denotes .
T1983 4904-4916 RB denotes Particularly
T1984 4917-4928 JJ denotes interesting
T1985 4929-4932 VBP denotes are
T1986 4933-4938 DT denotes those
T1987 4939-4943 NNP denotes Treg
T1988 4944-4949 NNS denotes cells
T1989 4950-4954 WDT denotes that
T1990 4955-4958 VBP denotes are
T1991 4959-4968 VBN denotes generated
T1992 4969-4971 IN denotes in
T1993 4972-4975 DT denotes the
T1994 4976-4985 NN denotes periphery
T1995 4986-4989 CC denotes and
T1996 4990-4994 RB denotes thus
T1997 4995-4998 VBP denotes are
T1998 4999-5008 JJ denotes potential
T1999 5009-5016 NNS denotes targets
T2000 5017-5020 IN denotes for
T2001 5021-5032 JJ denotes therapeutic
T2002 5033-5046 NNS denotes interventions
T2003 5046-5047 . denotes .
T2004 5048-5053 DT denotes These
T2005 5054-5061 JJ denotes induced
T2006 5062-5066 NNP denotes Treg
T2007 5067-5068 -LRB- denotes (
T2008 5068-5073 NNP denotes iTreg
T2009 5073-5074 -RRB- denotes )
T2010 5075-5080 NNS denotes cells
T2011 5081-5085 VBD denotes were
T2012 5086-5094 VBN denotes reported
T2013 5095-5097 TO denotes to
T2014 5098-5105 VB denotes express
T2015 5106-5111 CD denotes FOXP3
T2016 5112-5113 NNP denotes [
T2017 5113-5115 CD denotes 10
T2018 5115-5116 NNP denotes ]
T2019 5116-5117 . denotes .
T2020 5118-5121 DT denotes The
T2021 5122-5127 JJ denotes exact
T2022 5128-5138 NNS denotes mechanisms
T2023 5139-5141 IN denotes of
T2024 5142-5147 NNP denotes iTreg
T2025 5148-5158 NN denotes generation
T2026 5159-5162 VBP denotes are
T2027 5163-5170 JJ denotes unclear
T2028 5170-5171 , denotes ,
T2029 5172-5175 CC denotes but
T2030 5176-5177 NNP denotes T
T2031 5178-5182 NN denotes cell
T2032 5183-5191 NN denotes receptor
T2033 5192-5193 -LRB- denotes (
T2034 5193-5196 NNP denotes TCR
T2035 5196-5197 -RRB- denotes )
T2036 5198-5208 VBG denotes triggering
T2037 5209-5212 VBZ denotes has
T2038 5213-5217 VBN denotes been
T2039 5218-5223 VBN denotes shown
T2040 5224-5226 TO denotes to
T2041 5227-5233 VB denotes induce
T2042 5234-5239 CD denotes FOXP3
T2043 5240-5250 NN denotes expression
T2044 5251-5254 CC denotes and
T2045 5255-5266 JJ denotes suppressive
T2046 5267-5272 NNS denotes cells
T2047 5273-5275 IN denotes in
T2048 5276-5281 JJ denotes human
T2049 5282-5283 NNP denotes [
T2050 5283-5288 CD denotes 11,12
T2051 5288-5289 NNP denotes ]
T2052 5289-5290 , denotes ,
T2053 5291-5298 RB denotes however
T2054 5299-5302 DT denotes the
T2055 5303-5312 NN denotes phenotype
T2056 5313-5320 VBZ denotes appears
T2057 5321-5323 TO denotes to
T2058 5324-5326 VB denotes be
T2059 5327-5329 IN denotes of
T2060 5330-5339 JJ denotes transient
T2061 5340-5346 NN denotes nature
T2062 5347-5348 NNP denotes [
T2063 5348-5353 CD denotes 13,14
T2064 5353-5354 NNP denotes ]
T2065 5354-5355 . denotes .
T2066 5356-5361 NNP denotes TGF-β
T2067 5362-5365 VBZ denotes has
T2068 5366-5370 VBN denotes been
T2069 5371-5383 VBN denotes demonstrated
T2070 5384-5386 TO denotes to
T2071 5387-5389 VB denotes be
T2072 5390-5399 JJ denotes important
T2073 5400-5403 IN denotes for
T2074 5404-5407 DT denotes the
T2075 5408-5418 JJ denotes persistent
T2076 5419-5428 NN denotes induction
T2077 5429-5431 IN denotes of
T2078 5432-5437 DT denotes these
T2079 5438-5443 NNS denotes cells
T2080 5444-5446 IN denotes in
T2081 5447-5452 NN denotes vitro
T2082 5453-5456 CC denotes and
T2083 5457-5459 IN denotes in
T2084 5460-5464 NN denotes vivo
T2085 5464-5465 , denotes ,
T2086 5466-5471 IN denotes since
T2087 5472-5479 NNS denotes animals
T2088 5480-5487 VBG denotes lacking
T2089 5488-5491 DT denotes the
T2090 5492-5500 NNP denotes TGF-βRII
T2091 5501-5503 IN denotes on
T2092 5504-5505 NNP denotes T
T2093 5506-5511 NNS denotes cells
T2094 5512-5516 VBP denotes have
T2095 5517-5522 JJR denotes fewer
T2096 5523-5535 RB denotes peripherally
T2097 5536-5541 JJ denotes iTreg
T2098 5542-5547 NNS denotes cells
T2099 5548-5549 NNP denotes [
T2100 5549-5551 CD denotes 15
T2101 5551-5552 NNP denotes ]
T2102 5553-5556 CC denotes and
T2103 5557-5563 VBP denotes suffer
T2104 5564-5568 IN denotes from
T2105 5569-5570 DT denotes a
T2106 5571-5572 NNP denotes T
T2107 5573-5577 NN denotes cell
T2108 5578-5587 JJ denotes dependent
T2109 5588-5598 JJ denotes multiorgan
T2110 5599-5611 JJ denotes inflammatory
T2111 5612-5619 NN denotes disease
T2112 5620-5621 NNP denotes [
T2113 5621-5623 CD denotes 16
T2114 5623-5624 NNP denotes ]
T2115 5624-5625 . denotes .
T2116 5626-5634 IN denotes Although
T2117 5635-5638 DT denotes the
T2118 5639-5645 NN denotes effect
T2119 5646-5648 IN denotes of
T2120 5649-5654 NNP denotes TGF-β
T2121 5655-5657 IN denotes on
T2122 5658-5665 JJ denotes natural
T2123 5666-5669 CC denotes and
T2124 5670-5679 JJ denotes inducible
T2125 5680-5684 NNP denotes Treg
T2126 5685-5689 NN denotes cell
T2127 5690-5699 NN denotes induction
T2128 5700-5703 VBZ denotes has
T2129 5704-5708 VBN denotes been
T2130 5709-5721 VBN denotes demonstrated
T2131 5722-5732 RB denotes repeatedly
T2132 5733-5734 NNP denotes [
T2133 5734-5739 CD denotes 15,17
T2134 5739-5740 NNP denotes ]
T2135 5740-5741 , denotes ,
T2136 5742-5745 PRP$ denotes its
T2137 5746-5755 JJ denotes molecular
T2138 5756-5766 NNS denotes mechanisms
T2139 5767-5773 VBP denotes remain
T2140 5774-5776 TO denotes to
T2141 5777-5779 VB denotes be
T2142 5780-5790 VBN denotes identified
T2143 5790-5791 . denotes .
T2144 5792-5795 DT denotes The
T2145 5796-5803 JJ denotes current
T2146 5804-5809 NN denotes study
T2147 5810-5818 VBZ denotes provides
T2148 5819-5827 NN denotes evidence
T2149 5828-5832 IN denotes that
T2150 5833-5838 NNP denotes GATA3
T2151 5839-5842 CC denotes and
T2152 5843-5848 NNP denotes FOXP3
T2153 5849-5853 VBP denotes play
T2154 5854-5855 DT denotes a
T2155 5856-5867 JJ denotes competitive
T2156 5868-5872 NN denotes role
T2157 5873-5875 IN denotes in
T2158 5876-5881 NNP denotes iTreg
T2159 5882-5886 NN denotes cell
T2160 5887-5897 NN denotes commitment
T2161 5898-5900 IN denotes as
T2162 5901-5906 NNP denotes T-bet
T2163 5907-5910 CC denotes and
T2164 5911-5916 NNP denotes GATA3
T2165 5917-5920 IN denotes for
T2166 5921-5924 NNP denotes Th1
T2167 5925-5928 CC denotes and
T2168 5929-5932 NNP denotes Th2
T2169 5933-5948 NN denotes differentiation
T2170 5948-5949 , denotes ,
T2171 5950-5962 RB denotes respectively
T2172 5962-5963 . denotes .
T2173 5964-5966 PRP denotes We
T2174 5967-5971 VBP denotes show
T2175 5972-5976 IN denotes that
T2176 5977-5982 NNP denotes GATA3
T2177 5983-5991 VBZ denotes inhibits
T2178 5992-5997 CD denotes FOXP3
T2179 5998-6007 NN denotes induction
T2180 6008-6011 CC denotes and
T2181 6012-6016 IN denotes that
T2182 6017-6021 NN denotes IL-4
T2183 6022-6028 NNS denotes limits
T2184 6029-6034 CD denotes FOXP3
T2185 6035-6045 NN denotes expression
T2186 6046-6048 IN denotes in
T2187 6049-6050 DT denotes a
T2188 6051-6065 JJ denotes GATA3-mediated
T2189 6066-6069 NN denotes way
T2190 6069-6070 , denotes ,
T2191 6071-6075 DT denotes both
T2192 6076-6078 IN denotes in
T2193 6079-6084 NN denotes vitro
T2194 6085-6088 CC denotes and
T2195 6089-6091 IN denotes in
T2196 6092-6096 NN denotes vivo
T2197 6096-6097 . denotes .
T2198 6098-6100 PRP denotes We
T2199 6101-6105 RB denotes also
T2200 6106-6110 VBP denotes show
T2201 6111-6115 IN denotes that
T2202 6116-6121 CD denotes GATA3
T2203 6122-6130 RB denotes directly
T2204 6131-6136 VBZ denotes binds
T2205 6137-6139 TO denotes to
T2206 6140-6143 DT denotes the
T2207 6144-6149 CD denotes FOXP3
T2208 6150-6158 NN denotes promoter
T2209 6159-6162 CC denotes and
T2210 6163-6170 RB denotes thereby
T2211 6171-6179 VBZ denotes prevents
T2212 6180-6183 DT denotes the
T2213 6184-6193 NN denotes induction
T2214 6194-6196 IN denotes of
T2215 6197-6201 DT denotes this
T2216 6202-6206 NN denotes gene
T2217 6206-6207 , denotes ,
T2218 6208-6221 VBG denotes demonstrating
T2219 6222-6226 IN denotes that
T2220 6227-6230 JJ denotes Th2
T2221 6231-6246 NN denotes differentiation
T2222 6247-6256 NNS denotes overrules
T2223 6257-6262 NNP denotes iTreg
T2224 6263-6272 NN denotes induction
T2225 6272-6273 . denotes .
T3688 6284-6289 CD denotes FOXP3
T3689 6290-6299 NNP denotes Induction
T3690 6300-6302 IN denotes in
T3691 6303-6304 NNP denotes T
T3692 6305-6309 NNP denotes Cell
T3693 6310-6317 NNPS denotes Subsets
T3694 6318-6320 PRP denotes It
T3695 6321-6323 VBZ denotes is
T3696 6324-6331 VBN denotes assumed
T3697 6332-6336 IN denotes that
T3698 6337-6342 CD denotes FOXP3
T3699 6343-6353 NN denotes expression
T3700 6354-6357 MD denotes can
T3701 6358-6360 VB denotes be
T3702 6361-6368 VBN denotes induced
T3703 6369-6371 IN denotes in
T3704 6372-6385 JJ denotes nonregulatory
T3705 6386-6387 NNP denotes T
T3706 6388-6393 NNS denotes cells
T3707 6393-6394 , denotes ,
T3708 6395-6400 WDT denotes which
T3709 6401-6403 VBZ denotes is
T3710 6404-6406 DT denotes an
T3711 6407-6416 JJ denotes important
T3712 6417-6421 NN denotes step
T3713 6422-6424 IN denotes in
T3714 6425-6430 NNP denotes iTreg
T3715 6431-6435 NN denotes cell
T3716 6436-6451 NN denotes differentiation
T3717 6451-6452 . denotes .
T3718 6453-6460 RB denotes However
T3719 6460-6461 , denotes ,
T3720 6462-6464 PRP denotes it
T3721 6465-6467 VBZ denotes is
T3722 6468-6471 RB denotes not
T3723 6472-6477 VBN denotes known
T3724 6478-6480 IN denotes if
T3725 6481-6484 DT denotes all
T3726 6485-6488 CD denotes CD4
T3727 6488-6489 NN denotes +
T3728 6490-6491 NNP denotes T
T3729 6492-6497 NNS denotes cells
T3730 6498-6502 VBP denotes have
T3731 6503-6506 DT denotes the
T3732 6507-6511 JJ denotes same
T3733 6512-6520 NN denotes capacity
T3734 6521-6523 TO denotes to
T3735 6524-6531 VB denotes express
T3736 6532-6537 NNP denotes FOXP3
T3737 6537-6538 . denotes .
T3738 6539-6541 TO denotes To
T3739 6542-6553 VB denotes investigate
T3740 6554-6561 IN denotes whether
T3741 6562-6567 NNP denotes FOXP3
T3742 6568-6571 MD denotes can
T3743 6572-6574 VB denotes be
T3744 6575-6584 VBN denotes expressed
T3745 6585-6587 IN denotes by
T3746 6588-6591 DT denotes any
T3747 6592-6593 NNP denotes T
T3748 6594-6598 NN denotes cell
T3749 6599-6605 NN denotes subset
T3750 6606-6608 CC denotes or
T3751 6609-6611 IN denotes if
T3752 6612-6622 NN denotes expression
T3753 6623-6625 VBZ denotes is
T3754 6626-6636 VBN denotes restricted
T3755 6637-6639 TO denotes to
T3756 6640-6641 DT denotes a
T3757 6642-6650 JJ denotes distinct
T3758 6651-6658 NN denotes lineage
T3759 6658-6659 , denotes ,
T3760 6660-6665 CD denotes FOXP3
T3761 6666-6670 NNP denotes mRNA
T3762 6671-6681 NN denotes expression
T3763 6682-6685 VBD denotes was
T3764 6686-6694 VBN denotes analyzed
T3765 6695-6697 IN denotes in
T3766 6698-6705 RB denotes freshly
T3767 6706-6714 VBN denotes isolated
T3768 6715-6716 NNP denotes T
T3769 6717-6722 NNS denotes cells
T3770 6723-6727 JJ denotes such
T3771 6728-6730 IN denotes as
T3772 6731-6744 JJ denotes CD25-depleted
T3773 6745-6748 NNP denotes CD4
T3774 6748-6749 NN denotes +
T3775 6750-6755 NNS denotes cells
T3776 6755-6756 , denotes ,
T3777 6757-6763 NNP denotes CD45RA
T3778 6763-6764 NNP denotes +
T3779 6765-6770 JJ denotes naive
T3780 6771-6773 CC denotes or
T3781 6774-6780 CD denotes CD45RO
T3782 6780-6781 JJ denotes +
T3783 6782-6788 NN denotes memory
T3784 6789-6790 NNP denotes T
T3785 6791-6796 NNS denotes cells
T3786 6797-6798 -LRB- denotes (
T3787 6798-6804 NN denotes Figure
T3788 6805-6807 CD denotes 1A
T3789 6807-6808 -RRB- denotes )
T3790 6808-6809 , denotes ,
T3791 6810-6812 RB denotes as
T3792 6813-6817 RB denotes well
T3793 6818-6820 IN denotes as
T3794 6821-6822 NNP denotes T
T3795 6823-6828 NNS denotes cells
T3796 6829-6835 VBN denotes driven
T3797 6836-6838 IN denotes in
T3798 6839-6844 NN denotes vitro
T3799 6845-6851 IN denotes toward
T3800 6852-6855 NNP denotes Th1
T3801 6855-6856 , denotes ,
T3802 6857-6860 NNP denotes Th2
T3803 6860-6861 , denotes ,
T3804 6862-6864 CC denotes or
T3805 6865-6870 NNP denotes iTreg
T3806 6871-6881 NNS denotes phenotypes
T3807 6882-6883 -LRB- denotes (
T3808 6883-6889 NN denotes Figure
T3809 6890-6892 CD denotes 1B
T3810 6892-6893 : denotes ;
T3811 6894-6903 NN denotes phenotype
T3812 6904-6906 IN denotes on
T3813 6907-6913 NN denotes Figure
T3814 6914-6916 CD denotes S1
T3815 6916-6917 -RRB- denotes )
T3816 6917-6918 . denotes .
T3817 6919-6922 DT denotes The
T3818 6923-6926 NNP denotes CD4
T3819 6926-6927 NNP denotes +
T3820 6927-6931 NNP denotes CD25
T3821 6932-6933 , denotes ,
T3822 6934-6940 NNP denotes CD45RA
T3823 6940-6941 NNP denotes +
T3824 6941-6942 , denotes ,
T3825 6943-6949 NNP denotes CD45RO
T3826 6949-6950 NNP denotes +
T3827 6950-6951 , denotes ,
T3828 6952-6955 CC denotes and
T3829 6956-6959 NNP denotes CD4
T3830 6959-6960 NNP denotes +
T3831 6960-6966 NNP denotes CD45RO
T3832 6966-6967 NNP denotes +
T3833 6967-6971 NNP denotes CD25
T3834 6973-6977 VBD denotes were
T3835 6978-6982 JJ denotes able
T3836 6983-6985 TO denotes to
T3837 6986-6999 RB denotes significantly
T3838 7000-7006 VB denotes induce
T3839 7007-7012 CD denotes FOXP3
T3840 7013-7017 NNP denotes mRNA
T3841 7018-7020 IN denotes up
T3842 7021-7023 TO denotes to
T3843 7024-7031 JJ denotes 30-fold
T3844 7032-7036 IN denotes upon
T3845 7037-7040 NNP denotes TCR
T3846 7041-7051 NN denotes activation
T3847 7052-7055 CC denotes and
T3848 7056-7064 NN denotes addition
T3849 7065-7067 IN denotes of
T3850 7068-7073 NNP denotes TGF-β
T3851 7073-7074 . denotes .
T3852 7075-7078 CD denotes Th1
T3853 7079-7084 NNS denotes cells
T3854 7085-7091 VBD denotes showed
T3855 7092-7096 RB denotes only
T3856 7097-7098 DT denotes a
T3857 7099-7106 JJ denotes 10-fold
T3858 7107-7115 NN denotes increase
T3859 7115-7116 . denotes .
T3860 7117-7119 IN denotes In
T3861 7120-7128 NN denotes contrast
T3862 7128-7129 , denotes ,
T3863 7130-7133 JJ denotes Th2
T3864 7134-7139 NNS denotes cells
T3865 7140-7150 VBN denotes stimulated
T3866 7151-7156 IN denotes under
T3867 7157-7160 DT denotes the
T3868 7161-7165 JJ denotes same
T3869 7166-7176 NNS denotes conditions
T3870 7177-7180 VBD denotes did
T3871 7181-7184 RB denotes not
T3872 7185-7193 VB denotes increase
T3873 7194-7199 CD denotes FOXP3
T3874 7200-7210 NN denotes expression
T3875 7210-7211 . denotes .
T3876 7212-7215 DT denotes The
T3877 7216-7218 IN denotes in
T3878 7219-7224 NN denotes vitro
T3879 7225-7234 VBD denotes generated
T3880 7235-7240 NNP denotes iTreg
T3881 7241-7246 NNS denotes cells
T3882 7247-7251 VBD denotes were
T3883 7252-7258 JJ denotes unable
T3884 7259-7261 TO denotes to
T3885 7262-7269 JJ denotes further
T3886 7270-7281 JJ denotes up-regulate
T3887 7282-7287 NNP denotes FOXP3
T3888 7287-7288 , denotes ,
T3889 7289-7294 WDT denotes which
T3890 7295-7298 VBD denotes was
T3891 7299-7306 RB denotes already
T3892 7307-7309 IN denotes at
T3893 7310-7314 JJ denotes high
T3894 7315-7321 NNS denotes levels
T3895 7322-7327 IN denotes under
T3896 7328-7331 DT denotes the
T3897 7332-7339 NN denotes resting
T3898 7340-7350 NNS denotes conditions
T3899 7351-7352 -LRB- denotes (
T3900 7352-7358 NN denotes Figure
T3901 7359-7361 NN denotes 1B
T3902 7361-7362 , denotes ,
T3903 7363-7368 JJ denotes right
T3904 7369-7374 NN denotes panel
T3905 7374-7375 -RRB- denotes )
T3906 7375-7376 . denotes .
T3907 7387-7390 CD denotes Th2
T3908 7391-7396 NNS denotes Cells
T3909 7967-7970 VBP denotes are
T3910 7971-7976 VBN denotes known
T3911 7977-7979 TO denotes to
T3912 7980-7987 VB denotes produce
T3913 7988-7992 NN denotes IL-4
T3914 7993-7997 IN denotes upon
T3915 7998-8008 NN denotes activation
T3916 8008-8009 , denotes ,
T3917 8010-8015 WDT denotes which
T3918 8016-8019 MD denotes may
T3919 8020-8028 VB denotes interact
T3920 8029-8033 IN denotes with
T3921 8034-8039 JJ denotes TGF-β
T3922 8040-8049 VBG denotes signaling
T3923 8050-8053 CC denotes and
T3924 8054-8058 RB denotes thus
T3925 8059-8066 VB denotes prevent
T3926 8067-8072 CD denotes FOXP3
T3927 8073-8082 NN denotes induction
T3928 8082-8083 . denotes .
T3929 8084-8091 RB denotes However
T3930 8091-8092 , denotes ,
T3931 8093-8096 DT denotes the
T3932 8097-8111 NN denotes neutralization
T3933 8112-8114 IN denotes of
T3934 8115-8119 NN denotes IL-4
T3935 8120-8124 IN denotes with
T3936 8125-8126 DT denotes a
T3937 8127-8135 VBG denotes blocking
T3938 8136-8140 NN denotes IL-4
T3939 8141-8149 NN denotes antibody
T3940 8150-8153 VBD denotes did
T3941 8154-8157 RB denotes not
T3942 8158-8164 NN denotes rescue
T3943 8165-8170 CD denotes FOXP3
T3944 8171-8181 NN denotes expression
T3945 8182-8184 IN denotes in
T3946 8185-8188 DT denotes the
T3947 8189-8203 JJ denotes differentiated
T3948 8204-8207 JJ denotes Th2
T3949 8208-8213 NNS denotes cells
T3950 8214-8215 -LRB- denotes (
T3951 8215-8226 JJ denotes unpublished
T3952 8227-8231 NNS denotes data
T3953 8231-8232 -RRB- denotes )
T3954 8232-8233 . denotes .
T3955 8234-8239 DT denotes These
T3956 8240-8244 NNS denotes data
T3957 8245-8257 VBD denotes demonstrated
T3958 8258-8262 IN denotes that
T3959 8263-8266 JJ denotes Th2
T3960 8267-8272 NNS denotes cells
T3961 8273-8277 VBP denotes have
T3962 8278-8279 DT denotes a
T3963 8280-8287 JJ denotes limited
T3964 8288-8296 NN denotes capacity
T3965 8297-8299 TO denotes to
T3966 8300-8307 VB denotes express
T3967 8308-8313 NNP denotes FOXP3
T3968 8314-8315 -LRB- denotes (
T3969 8315-8321 NNP denotes Figure
T3970 8322-8324 NNP denotes 1B
T3971 8324-8325 -RRB- denotes )
T3972 8325-8326 . denotes .
T3973 8327-8330 DT denotes The
T3974 8331-8340 NN denotes inability
T3975 8341-8343 IN denotes of
T3976 8344-8347 JJ denotes Th2
T3977 8348-8353 NNS denotes cells
T3978 8354-8356 TO denotes to
T3979 8357-8364 VB denotes express
T3980 8365-8370 CD denotes FOXP3
T3981 8371-8374 VBD denotes was
T3982 8375-8379 RB denotes also
T3983 8380-8390 VBN denotes documented
T3984 8391-8393 IN denotes at
T3985 8394-8397 DT denotes the
T3986 8398-8409 JJ denotes single-cell
T3987 8410-8415 NN denotes level
T3988 8415-8416 , denotes ,
T3989 8417-8427 VBG denotes confirming
T3990 8428-8432 IN denotes that
T3991 8433-8436 JJ denotes Th2
T3992 8437-8442 NNS denotes cells
T3993 8443-8447 VBP denotes lack
T3994 8448-8453 CD denotes FOXP3
T3995 8454-8464 NN denotes expression
T3996 8465-8466 -LRB- denotes (
T3997 8466-8472 NN denotes Figure
T3998 8473-8475 NN denotes 2A
T3999 8475-8476 -RRB- denotes )
T4000 8476-8477 . denotes .
T4001 8478-8482 RB denotes Only
T4002 8483-8488 JJ denotes iTreg
T4003 8489-8494 NNS denotes cells
T4004 8495-8504 VBD denotes expressed
T4005 8505-8510 CD denotes FOXP3
T4006 8511-8513 IN denotes in
T4007 8514-8521 VBG denotes resting
T4008 8522-8532 NNS denotes conditions
T4009 8532-8533 . denotes .
T4010 8534-8547 RB denotes Interestingly
T4011 8547-8548 , denotes ,
T4012 8549-8551 PRP denotes we
T4013 8552-8560 VBD denotes observed
T4014 8561-8565 IN denotes that
T4015 8566-8573 VBG denotes resting
T4016 8574-8579 NNP denotes iTreg
T4017 8580-8585 NNS denotes cells
T4018 8586-8593 VBP denotes express
T4019 8594-8599 CD denotes FOXP3
T4020 8600-8603 CC denotes but
T4021 8604-8608 VB denotes show
T4022 8609-8612 JJ denotes low
T4023 8613-8617 NNS denotes CD25
T4024 8618-8625 VBP denotes surface
T4025 8626-8636 NN denotes expression
T4026 8636-8637 . denotes .
T4027 8638-8646 VBN denotes Repeated
T4028 8647-8655 NN denotes exposure
T4029 8656-8658 TO denotes to
T4030 8659-8664 NNP denotes TGF-β
T4031 8665-8668 VBD denotes did
T4032 8669-8672 RB denotes not
T4033 8673-8680 JJ denotes further
T4034 8681-8689 NN denotes increase
T4035 8690-8693 DT denotes the
T4036 8694-8699 CD denotes FOXP3
T4037 8700-8710 NN denotes expression
T4038 8711-8713 IN denotes in
T4039 8714-8717 DT denotes the
T4040 8718-8723 NNP denotes iTreg
T4041 8724-8731 NN denotes lineage
T4042 8732-8735 CC denotes but
T4043 8736-8747 RB denotes transiently
T4044 8748-8755 VBD denotes induced
T4045 8756-8761 CD denotes FOXP3
T4046 8762-8772 NN denotes expression
T4047 8773-8775 IN denotes in
T4048 8776-8779 JJ denotes Th1
T4049 8780-8785 NNS denotes cells
T4050 8785-8786 . denotes .
T4051 8787-8796 RB denotes Naturally
T4052 8797-8806 VBG denotes occurring
T4053 8807-8810 JJ denotes Th2
T4054 8811-8816 NNS denotes cells
T4055 8817-8821 JJ denotes such
T4056 8822-8824 IN denotes as
T4057 8825-8830 NNP denotes CRTH2
T4058 8830-8831 NNP denotes +
T4059 8832-8833 NNP denotes T
T4060 8834-8839 NNS denotes cells
T4061 8839-8840 , denotes ,
T4062 8841-8842 NNP denotes T
T4063 8843-8848 NNS denotes cells
T4064 8849-8857 VBD denotes isolated
T4065 8858-8867 VBG denotes according
T4066 8868-8870 TO denotes to
T4067 8871-8876 PRP$ denotes their
T4068 8877-8881 NN denotes IL-4
T4069 8882-8891 NN denotes secretion
T4070 8891-8892 , denotes ,
T4071 8893-8895 CC denotes or
T4072 8896-8898 DT denotes an
T4073 8899-8903 NN denotes IL-4
T4074 8904-8913 VBG denotes producing
T4075 8914-8915 NNP denotes T
T4076 8916-8920 NN denotes cell
T4077 8921-8926 NN denotes clone
T4078 8927-8928 -LRB- denotes (
T4079 8928-8931 CD denotes BR8
T4080 8931-8932 -RRB- denotes )
T4081 8933-8937 VBD denotes were
T4082 8938-8942 RB denotes also
T4083 8943-8950 VBG denotes lacking
T4084 8951-8956 CD denotes FOXP3
T4085 8957-8967 NN denotes expression
T4086 8968-8969 -LRB- denotes (
T4087 8969-8975 NN denotes Figure
T4088 8976-8978 NN denotes 2B
T4089 8978-8979 -RRB- denotes )
T4090 8979-8980 . denotes .
T4091 8981-8992 RB denotes Furthermore
T4092 8993-8998 JJ denotes TGF-β
T4093 8999-9007 VBD denotes mediated
T4094 9008-9013 CD denotes FOXP3
T4095 9014-9023 NN denotes induction
T4096 9024-9030 VBD denotes failed
T4097 9031-9033 IN denotes in
T4098 9034-9039 DT denotes these
T4099 9040-9045 NNS denotes cells
T4100 9046-9048 IN denotes in
T4101 9049-9057 NN denotes contrast
T4102 9058-9060 TO denotes to
T4103 9061-9064 DT denotes the
T4104 9065-9070 JJ denotes naive
T4105 9071-9072 NNP denotes T
T4106 9073-9078 NNS denotes cells
T4107 9079-9080 -LRB- denotes (
T4108 9080-9086 NN denotes Figure
T4109 9087-9089 NN denotes 2B
T4110 9089-9090 -RRB- denotes )
T4111 9090-9091 . denotes .
T4112 9092-9099 IN denotes Because
T4113 9100-9104 NN denotes IL-4
T4114 9105-9107 VBZ denotes is
T4115 9108-9111 DT denotes the
T4116 9112-9115 JJ denotes key
T4117 9116-9119 JJ denotes Th2
T4118 9120-9128 NN denotes cytokine
T4119 9128-9129 , denotes ,
T4120 9130-9133 DT denotes the
T4121 9134-9144 NN denotes expression
T4122 9145-9147 IN denotes of
T4123 9148-9152 NN denotes IL-4
T4124 9153-9156 CC denotes and
T4125 9157-9162 CD denotes FOXP3
T4126 9163-9165 IN denotes in
T4127 9166-9173 RB denotes freshly
T4128 9174-9182 VBN denotes isolated
T4129 9183-9186 NNP denotes CD4
T4130 9186-9187 CD denotes +
T4131 9188-9189 NNP denotes T
T4132 9190-9195 NNS denotes cells
T4133 9196-9199 VBD denotes was
T4134 9200-9208 VBN denotes analyzed
T4135 9209-9211 IN denotes by
T4136 9212-9224 NN denotes fluorescence
T4137 9225-9234 VBN denotes activated
T4138 9235-9239 NN denotes cell
T4139 9240-9247 VBG denotes sorting
T4140 9248-9249 -LRB- denotes (
T4141 9249-9253 NNP denotes FACS
T4142 9253-9254 -RRB- denotes )
T4143 9254-9255 . denotes .
T4144 9256-9260 NN denotes IL-4
T4145 9261-9271 VBG denotes expressing
T4146 9272-9277 NNS denotes cells
T4147 9278-9282 VBD denotes were
T4148 9283-9287 RBS denotes most
T4149 9288-9296 JJ denotes abundant
T4150 9297-9302 IN denotes among
T4151 9303-9309 CD denotes CD45RO
T4152 9309-9310 CD denotes +
T4153 9310-9314 NNS denotes CD25
T4154 9316-9321 NNS denotes cells
T4155 9321-9322 , denotes ,
T4156 9323-9328 WDT denotes which
T4157 9329-9332 VBD denotes did
T4158 9333-9336 RB denotes not
T4159 9337-9347 JJ denotes co-express
T4160 9348-9353 NNP denotes FOXP3
T4161 9354-9355 -LRB- denotes (
T4162 9355-9361 NNP denotes Figure
T4163 9362-9364 NNP denotes 3A
T4164 9364-9365 , denotes ,
T4165 9366-9370 VBD denotes left
T4166 9371-9376 NN denotes panel
T4167 9376-9377 -RRB- denotes )
T4168 9377-9378 . denotes .
T4169 9379-9381 IN denotes In
T4170 9382-9390 NN denotes contrast
T4171 9390-9391 , denotes ,
T4172 9392-9398 NNP denotes CD45RO
T4173 9398-9399 NNP denotes +
T4174 9399-9403 NNP denotes CD25
T4175 9403-9404 VBD denotes +
T4176 9405-9410 NNS denotes cells
T4177 9411-9421 RB denotes abundantly
T4178 9422-9431 VBD denotes expressed
T4179 9432-9437 CD denotes FOXP3
T4180 9437-9438 , denotes ,
T4181 9439-9444 IN denotes while
T4182 9445-9452 VBG denotes lacking
T4183 9453-9457 NN denotes IL-4
T4184 9458-9459 -LRB- denotes (
T4185 9459-9465 NNP denotes Figure
T4186 9466-9468 NNP denotes 3A
T4187 9468-9469 , denotes ,
T4188 9470-9475 JJ denotes right
T4189 9476-9481 NN denotes panel
T4190 9481-9482 -RRB- denotes )
T4191 9482-9483 . denotes .
T4192 9484-9486 IN denotes As
T4193 9487-9492 VBN denotes shown
T4194 9493-9495 IN denotes in
T4195 9496-9502 NN denotes Figure
T4196 9503-9505 NN denotes 3B
T4197 9505-9506 , denotes ,
T4198 9507-9510 DT denotes the
T4199 9511-9520 NN denotes frequency
T4200 9521-9523 IN denotes of
T4201 9524-9527 DT denotes the
T4202 9528-9532 NN denotes IL-4
T4203 9532-9533 NN denotes +
T4204 9534-9539 NNS denotes cells
T4205 9540-9543 VBD denotes was
T4206 9544-9550 RB denotes always
T4207 9551-9556 IN denotes below
T4208 9557-9558 CD denotes 1
T4209 9558-9559 NN denotes %
T4210 9560-9562 IN denotes in
T4211 9563-9566 DT denotes the
T4212 9567-9572 CD denotes FOXP3
T4213 9572-9573 NN denotes +
T4214 9574-9579 NNS denotes cells
T4215 9580-9585 RB denotes close
T4216 9586-9588 TO denotes to
T4217 9589-9592 DT denotes the
T4218 9593-9603 NN denotes background
T4219 9603-9604 . denotes .
T4220 9605-9608 DT denotes The
T4221 9609-9613 NN denotes IL-4
T4222 9613-9614 NN denotes +
T4223 9615-9620 NNS denotes cells
T4224 9621-9625 VBD denotes were
T4225 9626-9634 VBN denotes confined
T4226 9635-9637 TO denotes to
T4227 9638-9641 DT denotes the
T4228 9642-9647 CD denotes FOXP3
T4229 9649-9654 NNS denotes cells
T4230 9654-9655 , denotes ,
T4231 9656-9658 IN denotes as
T4232 9659-9664 VBN denotes shown
T4233 9665-9668 IN denotes for
T4234 9669-9672 DT denotes the
T4235 9673-9679 NNP denotes CD45RO
T4236 9679-9680 NNP denotes +
T4237 9680-9684 NNP denotes CD25
T4238 9685-9686 , denotes ,
T4239 9687-9693 NNP denotes CD45RO
T4240 9693-9694 NNP denotes +
T4241 9694-9698 NNP denotes CD25
T4242 9698-9699 NNP denotes +
T4243 9699-9700 , denotes ,
T4244 9701-9704 CC denotes and
T4245 9705-9711 NNP denotes CD45RO
T4246 9711-9712 NNP denotes +
T4247 9712-9716 NNP denotes CD25
T4248 9716-9717 RB denotes +
T4249 9717-9721 JJ denotes high
T4250 9722-9727 NNS denotes cells
T4251 9728-9729 -LRB- denotes (
T4252 9729-9735 NN denotes Figure
T4253 9736-9738 NN denotes 3B
T4254 9738-9739 -RRB- denotes )
T4255 9739-9740 . denotes .
T4256 9741-9743 IN denotes In
T4257 9744-9752 NN denotes addition
T4258 9752-9753 , denotes ,
T4259 9754-9761 CC denotes neither
T4260 9762-9765 DT denotes the
T4261 9766-9769 JJ denotes Th2
T4262 9770-9775 NN denotes clone
T4263 9776-9777 -LRB- denotes (
T4264 9777-9780 CD denotes BR8
T4265 9780-9781 -RRB- denotes )
T4266 9782-9785 CC denotes nor
T4267 9786-9791 JJ denotes CRTH2
T4268 9792-9797 NNS denotes cells
T4269 9798-9811 RB denotes significantly
T4270 9812-9821 VBD denotes expressed
T4271 9822-9827 NNP denotes FOXP3
T4272 9828-9829 -LRB- denotes (
T4273 9829-9835 NNP denotes Figure
T4274 9836-9838 NNP denotes 3C
T4275 9838-9839 -RRB- denotes )
T4276 9839-9840 . denotes .
T4277 9841-9846 NNS denotes Cells
T4278 9847-9855 VBD denotes enriched
T4279 9856-9859 IN denotes for
T4280 9860-9865 PRP$ denotes their
T4281 9866-9870 NN denotes IL-4
T4282 9871-9880 NN denotes secretion
T4283 9881-9886 VBG denotes using
T4284 9887-9890 DT denotes the
T4285 9891-9899 JJ denotes magnetic
T4286 9900-9904 NN denotes cell
T4287 9905-9914 NN denotes isolation
T4288 9915-9925 NN denotes technology
T4289 9926-9935 VBD denotes contained
T4290 9936-9940 DT denotes some
T4291 9941-9957 JJ denotes FOXP3-expressing
T4292 9958-9963 NNS denotes cells
T4293 9963-9964 , denotes ,
T4294 9965-9968 CC denotes but
T4295 9969-9980 RB denotes importantly
T4296 9980-9981 , denotes ,
T4297 9982-9985 DT denotes the
T4298 9986-9996 NN denotes expression
T4299 9997-10000 VBD denotes did
T4300 10001-10004 RB denotes not
T4301 10005-10012 VB denotes overlap
T4302 10012-10013 . denotes .
T4303 10014-10019 VBN denotes Taken
T4304 10020-10028 RB denotes together
T4305 10028-10029 , denotes ,
T4306 10030-10035 DT denotes these
T4307 10036-10040 NNS denotes data
T4308 10041-10049 VBP denotes indicate
T4309 10050-10054 IN denotes that
T4310 10055-10060 CD denotes FOXP3
T4311 10061-10064 VBD denotes was
T4312 10065-10068 RB denotes not
T4313 10069-10078 VBN denotes expressed
T4314 10079-10081 IN denotes by
T4315 10082-10085 JJ denotes Th2
T4316 10086-10091 NNS denotes cells
T4317 10092-10095 CC denotes and
T4318 10096-10099 VBD denotes was
T4319 10100-10103 RB denotes not
T4320 10104-10113 JJ denotes inducible
T4321 10114-10116 IN denotes in
T4322 10117-10122 DT denotes those
T4323 10123-10128 NNS denotes cells
T4324 10128-10129 . denotes .
T5565 11403-11408 CD denotes FOXP3
T5566 11409-11412 CC denotes and
T5567 11413-11418 NNP denotes GATA3
T5568 11419-11427 NNPS denotes Kinetics
T5569 11428-11430 IN denotes in
T5570 11431-11446 NNP denotes Differentiating
T5571 11447-11452 NNP denotes Cells
T5572 11453-11456 NNP denotes The
T5573 11457-11464 JJ denotes limited
T5574 11465-11473 NN denotes capacity
T5575 11474-11476 IN denotes of
T5576 11477-11491 JJ denotes differentiated
T5577 11492-11500 NN denotes effector
T5578 11501-11506 NNS denotes cells
T5579 11507-11509 TO denotes to
T5580 11510-11516 VB denotes induce
T5581 11517-11522 CD denotes FOXP3
T5582 11523-11533 NN denotes expression
T5583 11534-11542 VBZ denotes suggests
T5584 11543-11547 IN denotes that
T5585 11548-11553 NNP denotes iTreg
T5586 11554-11563 NN denotes induction
T5587 11564-11567 VBZ denotes has
T5588 11568-11570 TO denotes to
T5589 11571-11576 VB denotes occur
T5590 11577-11583 IN denotes before
T5591 11584-11592 NN denotes effector
T5592 11593-11594 NNP denotes T
T5593 11595-11599 NN denotes cell
T5594 11600-11615 NN denotes differentiation
T5595 11616-11622 VBZ denotes occurs
T5596 11622-11623 . denotes .
T5597 11624-11633 RB denotes Therefore
T5598 11633-11634 , denotes ,
T5599 11635-11637 PRP denotes we
T5600 11638-11646 VBD denotes analyzed
T5601 11647-11650 DT denotes the
T5602 11651-11661 NN denotes expression
T5603 11662-11664 IN denotes of
T5604 11665-11670 CD denotes FOXP3
T5605 11671-11674 CC denotes and
T5606 11675-11680 CD denotes GATA3
T5607 11681-11687 IN denotes during
T5608 11688-11691 DT denotes the
T5609 11692-11707 NN denotes differentiation
T5610 11708-11710 IN denotes of
T5611 11711-11716 JJ denotes naive
T5612 11717-11720 NNP denotes CD4
T5613 11721-11722 NNP denotes T
T5614 11723-11728 NNS denotes cells
T5615 11729-11733 IN denotes into
T5616 11734-11737 NNP denotes Th0
T5617 11738-11739 -LRB- denotes (
T5618 11739-11746 JJ denotes neutral
T5619 11746-11747 , denotes ,
T5620 11748-11757 JJ denotes anti-IL-4
T5621 11757-11758 , denotes ,
T5622 11759-11769 JJ denotes anti-IFN-γ
T5623 11769-11770 , denotes ,
T5624 11771-11774 CC denotes and
T5625 11775-11779 NNS denotes anti
T5626 11780-11785 NN denotes IL-12
T5627 11785-11786 -RRB- denotes )
T5628 11786-11787 , denotes ,
T5629 11788-11791 NNP denotes Th2
T5630 11791-11792 , denotes ,
T5631 11793-11796 CC denotes and
T5632 11797-11802 NNP denotes iTreg
T5633 11803-11813 NNS denotes phenotypes
T5634 11813-11814 . denotes .
T5635 11815-11820 IN denotes After
T5636 11821-11831 NN denotes initiation
T5637 11832-11834 IN denotes of
T5638 11835-11838 DT denotes the
T5639 11839-11854 NN denotes differentiation
T5640 11855-11862 NN denotes process
T5641 11862-11863 , denotes ,
T5642 11864-11869 CD denotes FOXP3
T5643 11870-11873 CC denotes and
T5644 11874-11879 CD denotes GATA3
T5645 11880-11886 VBD denotes showed
T5646 11887-11888 DT denotes a
T5647 11889-11896 JJ denotes similar
T5648 11897-11907 NN denotes expression
T5649 11908-11915 NN denotes kinetic
T5650 11916-11922 IN denotes within
T5651 11923-11926 DT denotes the
T5652 11927-11932 JJ denotes first
T5653 11933-11934 CD denotes 3
T5654 11935-11936 LS denotes d
T5655 11936-11937 , denotes ,
T5656 11938-11943 WDT denotes which
T5657 11944-11947 VBP denotes are
T5658 11948-11958 VBN denotes considered
T5659 11959-11961 TO denotes to
T5660 11962-11964 VB denotes be
T5661 11965-11973 JJ denotes critical
T5662 11974-11976 IN denotes in
T5663 11977-11978 NNP denotes T
T5664 11979-11983 NN denotes cell
T5665 11984-11994 NN denotes commitment
T5666 11995-11996 NNP denotes [
T5667 11996-11998 CD denotes 18
T5668 11998-11999 NNP denotes ]
T5669 11999-12000 . denotes .
T5670 12001-12006 IN denotes Under
T5671 12007-12010 JJ denotes Th2
T5672 12011-12026 NN denotes differentiation
T5673 12027-12037 NNS denotes conditions
T5674 12037-12038 , denotes ,
T5675 12039-12044 NNP denotes FOXP3
T5676 12045-12049 NNP denotes mRNA
T5677 12050-12060 NN denotes expression
T5678 12061-12070 VBD denotes increased
T5679 12071-12075 RB denotes only
T5680 12076-12086 RB denotes marginally
T5681 12087-12088 -LRB- denotes (
T5682 12088-12094 NN denotes Figure
T5683 12095-12097 NN denotes 4A
T5684 12097-12098 , denotes ,
T5685 12099-12103 VBD denotes left
T5686 12104-12109 NN denotes panel
T5687 12109-12110 -RRB- denotes )
T5688 12110-12111 . denotes .
T5689 12112-12116 RB denotes Thus
T5690 12116-12117 , denotes ,
T5691 12118-12126 IN denotes although
T5692 12127-12132 NNP denotes GATA3
T5693 12133-12136 CC denotes and
T5694 12137-12142 NNP denotes FOXP3
T5695 12143-12149 VBD denotes showed
T5696 12150-12157 JJ denotes similar
T5697 12158-12166 NNS denotes kinetics
T5698 12166-12167 , denotes ,
T5699 12168-12173 PRP$ denotes their
T5700 12174-12184 NN denotes expression
T5701 12185-12194 VBZ denotes polarizes
T5702 12195-12197 IN denotes at
T5703 12198-12201 DT denotes the
T5704 12202-12205 NN denotes end
T5705 12206-12208 IN denotes of
T5706 12209-12212 DT denotes the
T5707 12213-12228 NN denotes differentiation
T5708 12229-12236 NN denotes process
T5709 12237-12241 WRB denotes when
T5710 12242-12247 NNS denotes cells
T5711 12248-12252 VBD denotes were
T5712 12253-12261 VBN denotes cultured
T5713 12262-12269 IN denotes towards
T5714 12270-12273 NNP denotes Th2
T5715 12274-12276 CC denotes or
T5716 12277-12282 NNP denotes iTreg
T5717 12283-12288 NNS denotes cells
T5718 12288-12289 , denotes ,
T5719 12290-12302 RB denotes respectively
T5720 12303-12304 -LRB- denotes (
T5721 12304-12310 NN denotes Figure
T5722 12311-12313 NN denotes 4A
T5723 12314-12317 CC denotes and
T5724 12318-12320 NN denotes 4B
T5725 12320-12321 -RRB- denotes )
T5726 12321-12322 . denotes .
T5727 12323-12336 RB denotes Interestingly
T5728 12336-12337 , denotes ,
T5729 12338-12341 DT denotes the
T5730 12342-12345 JJ denotes Th0
T5731 12346-12351 NNS denotes cells
T5732 12352-12356 VBD denotes were
T5733 12357-12367 VBG denotes expressing
T5734 12368-12372 RBR denotes more
T5735 12373-12378 JJ denotes FOXP3
T5736 12379-12383 IN denotes than
T5737 12384-12387 DT denotes the
T5738 12388-12391 JJ denotes Th2
T5739 12392-12397 NNS denotes cells
T5740 12397-12398 , denotes ,
T5741 12399-12402 CC denotes but
T5742 12403-12412 VBD denotes expressed
T5743 12413-12416 JJ denotes low
T5744 12417-12423 NNS denotes levels
T5745 12424-12426 IN denotes of
T5746 12427-12432 NNP denotes GATA3
T5747 12432-12433 : denotes ;
T5748 12434-12441 RB denotes however
T5749 12441-12442 , denotes ,
T5750 12443-12446 DT denotes the
T5751 12447-12454 NN denotes protein
T5752 12455-12465 NN denotes expression
T5753 12466-12474 RB denotes slightly
T5754 12475-12483 VBD denotes differed
T5755 12484-12488 IN denotes from
T5756 12489-12493 NNP denotes mRNA
T5757 12494-12504 NN denotes expression
T5758 12504-12505 , denotes ,
T5759 12506-12516 VBG denotes suggesting
T5760 12517-12521 RB denotes also
T5761 12522-12539 JJ denotes posttranslational
T5762 12540-12550 NN denotes regulation
T5763 12551-12554 CC denotes and
T5764 12555-12566 NN denotes degradation
T5765 12567-12569 IN denotes as
T5766 12570-12579 JJ denotes potential
T5767 12580-12590 JJ denotes additional
T5768 12591-12601 NNS denotes mechanisms
T5769 12602-12604 IN denotes in
T5770 12605-12608 DT denotes the
T5771 12609-12624 NN denotes differentiation
T5772 12625-12632 NN denotes process
T5773 12632-12633 . denotes .
T5774 12634-12637 DT denotes The
T5775 12638-12647 NN denotes phenotype
T5776 12648-12650 IN denotes of
T5777 12651-12656 NNP denotes iTreg
T5778 12657-12662 NNS denotes cells
T5779 12663-12671 VBD denotes included
T5780 12672-12674 DT denotes an
T5781 12675-12682 JJ denotes anergic
T5782 12683-12692 NN denotes phenotype
T5783 12693-12697 IN denotes upon
T5784 12698-12706 JJ denotes anti-CD3
T5785 12707-12721 NN denotes re-stimulation
T5786 12722-12723 -LRB- denotes (
T5787 12723-12729 NN denotes Figure
T5788 12730-12733 CD denotes S1A
T5789 12733-12734 -RRB- denotes )
T5790 12734-12735 , denotes ,
T5791 12736-12741 NNP denotes CD103
T5792 12741-12742 , denotes ,
T5793 12743-12749 NNP denotes CTLA-4
T5794 12749-12750 , denotes ,
T5795 12751-12755 NNP denotes GITR
T5796 12755-12756 , denotes ,
T5797 12757-12760 CC denotes and
T5798 12761-12765 NN denotes PD-1
T5799 12766-12773 NN denotes surface
T5800 12774-12784 NN denotes expression
T5801 12785-12786 -LRB- denotes (
T5802 12786-12792 NN denotes Figure
T5803 12793-12796 CD denotes S1B
T5804 12796-12797 -RRB- denotes )
T5805 12797-12798 . denotes .
T5806 12799-12801 IN denotes On
T5807 12802-12805 DT denotes the
T5808 12806-12817 JJ denotes single-cell
T5809 12818-12823 NN denotes level
T5810 12823-12824 , denotes ,
T5811 12825-12827 PRP denotes it
T5812 12828-12831 MD denotes can
T5813 12832-12834 VB denotes be
T5814 12835-12839 VBN denotes seen
T5815 12840-12844 IN denotes that
T5816 12845-12850 NNS denotes cells
T5817 12851-12859 VBP denotes progress
T5818 12860-12867 IN denotes through
T5819 12868-12869 DT denotes a
T5820 12870-12880 NN denotes transition
T5821 12881-12886 NN denotes phase
T5822 12886-12887 , denotes ,
T5823 12888-12893 WRB denotes where
T5824 12894-12899 NNP denotes GATA3
T5825 12900-12903 CC denotes and
T5826 12904-12909 NNP denotes FOXP3
T5827 12910-12920 NN denotes expression
T5828 12921-12928 NN denotes coexist
T5829 12929-12931 TO denotes to
T5830 12932-12936 DT denotes some
T5831 12937-12943 NN denotes degree
T5832 12944-12946 IN denotes in
T5833 12947-12950 DT denotes the
T5834 12951-12955 JJ denotes same
T5835 12956-12961 NNS denotes cells
T5836 12961-12962 , denotes ,
T5837 12963-12968 WDT denotes which
T5838 12969-12971 VBZ denotes is
T5839 12972-12980 VBN denotes resolved
T5840 12981-12983 IN denotes in
T5841 12984-12989 NNP denotes iTreg
T5842 12990-12995 NNS denotes cells
T5843 12996-13001 IN denotes after
T5844 13002-13003 CD denotes 7
T5845 13004-13005 LS denotes d
T5846 13006-13007 -LRB- denotes (
T5847 13007-13013 NN denotes Figure
T5848 13014-13016 NN denotes 4B
T5849 13016-13017 -RRB- denotes )
T5850 13017-13018 . denotes .
T5851 13019-13024 VBN denotes Taken
T5852 13025-13033 RB denotes together
T5853 13033-13034 , denotes ,
T5854 13035-13040 DT denotes these
T5855 13041-13045 NNS denotes data
T5856 13046-13058 VBD denotes demonstrated
T5857 13059-13063 IN denotes that
T5858 13064-13067 JJ denotes Th2
T5859 13068-13073 NNS denotes cells
T5860 13074-13078 VBP denotes have
T5861 13079-13083 VBN denotes lost
T5862 13084-13089 PRP$ denotes their
T5863 13090-13098 NN denotes capacity
T5864 13099-13101 TO denotes to
T5865 13102-13109 VB denotes express
T5866 13110-13115 CD denotes FOXP3
T5867 13116-13119 CC denotes and
T5868 13120-13126 VBD denotes showed
T5869 13127-13131 IN denotes that
T5870 13132-13135 NNP denotes Th2
T5871 13136-13139 CC denotes and
T5872 13140-13145 NNP denotes iTreg
T5873 13146-13151 NNS denotes cells
T5874 13152-13157 VBP denotes arise
T5875 13158-13162 IN denotes from
T5876 13163-13166 CD denotes two
T5877 13167-13176 JJ denotes different
T5878 13177-13192 NN denotes differentiation
T5879 13193-13201 NNS denotes pathways
T5880 13201-13202 . denotes .
T7041 13785-13789 NN denotes IL-4
T7042 13790-13798 NNS denotes Inhibits
T7043 13799-13813 JJ denotes TGF-β-Mediated
T7044 13814-13819 NNP denotes iTreg
T7045 13820-13830 NNP denotes Commitment
T7046 13831-13835 NN denotes IL-4
T7047 13836-13843 VBZ denotes induces
T7048 13844-13859 NN denotes differentiation
T7049 13860-13862 IN denotes of
T7050 13863-13868 JJ denotes naive
T7051 13869-13870 NNP denotes T
T7052 13871-13876 NNS denotes cells
T7053 13876-13877 , denotes ,
T7054 13878-13882 IN denotes upon
T7055 13883-13890 NN denotes antigen
T7056 13891-13900 NN denotes encounter
T7057 13900-13901 , denotes ,
T7058 13902-13906 IN denotes into
T7059 13907-13910 DT denotes the
T7060 13911-13914 NNP denotes Th2
T7061 13915-13919 NN denotes cell
T7062 13920-13927 NN denotes lineage
T7063 13927-13928 . denotes .
T7064 13929-13931 PRP denotes We
T7065 13932-13941 RB denotes therefore
T7066 13942-13947 VBD denotes asked
T7067 13948-13955 IN denotes whether
T7068 13956-13960 NN denotes IL-4
T7069 13961-13963 VBZ denotes is
T7070 13964-13968 JJ denotes able
T7071 13969-13971 TO denotes to
T7072 13972-13979 VB denotes inhibit
T7073 13980-13985 JJ denotes TGF-β
T7074 13986-13995 NN denotes induction
T7075 13996-13998 IN denotes of
T7076 13999-14004 CD denotes FOXP3
T7077 14005-14011 IN denotes during
T7078 14012-14015 DT denotes the
T7079 14016-14023 NN denotes priming
T7080 14024-14026 IN denotes of
T7081 14027-14032 JJ denotes naive
T7082 14033-14034 NNP denotes T
T7083 14035-14040 NNS denotes cells
T7084 14040-14041 . denotes .
T7085 14042-14047 NNP denotes Human
T7086 14048-14051 NNP denotes CD4
T7087 14051-14052 NNP denotes +
T7088 14052-14058 NNP denotes CD45RA
T7089 14058-14059 VBD denotes +
T7090 14060-14061 NNP denotes T
T7091 14062-14067 NNS denotes cells
T7092 14068-14072 VBD denotes were
T7093 14073-14082 VBN denotes activated
T7094 14083-14087 IN denotes with
T7095 14088-14099 JJ denotes plate-bound
T7096 14100-14108 JJ denotes anti-CD3
T7097 14108-14109 NN denotes /
T7098 14109-14113 CD denotes CD28
T7099 14114-14116 IN denotes in
T7100 14117-14120 DT denotes the
T7101 14121-14129 NN denotes presence
T7102 14130-14132 IN denotes of
T7103 14133-14138 NNP denotes TGF-β
T7104 14139-14145 CC denotes and/or
T7105 14146-14150 NN denotes IL-4
T7106 14151-14154 CC denotes and
T7107 14155-14164 VBN denotes harvested
T7108 14165-14170 IN denotes after
T7109 14171-14172 CD denotes 5
T7110 14173-14175 NN denotes d.
T7111 14176-14180 NN denotes IL-4
T7112 14181-14192 RB denotes efficiently
T7113 14193-14202 VBD denotes repressed
T7114 14203-14206 DT denotes the
T7115 14207-14212 NNP denotes TGF-β
T7116 14213-14221 VBD denotes mediated
T7117 14222-14231 NN denotes induction
T7118 14232-14234 IN denotes of
T7119 14235-14240 CD denotes FOXP3
T7120 14241-14251 NN denotes expression
T7121 14252-14253 -LRB- denotes (
T7122 14253-14259 NN denotes Figure
T7123 14260-14262 NN denotes 5A
T7124 14262-14263 -RRB- denotes )
T7125 14264-14266 IN denotes in
T7126 14267-14268 DT denotes a
T7127 14269-14292 JJ denotes concentration-dependent
T7128 14293-14299 NN denotes manner
T7129 14300-14301 -LRB- denotes (
T7130 14301-14307 NN denotes Figure
T7131 14308-14310 NN denotes 5B
T7132 14310-14311 -RRB- denotes )
T7133 14311-14312 . denotes .
T7134 14313-14316 NNP denotes Low
T7135 14317-14323 NNS denotes levels
T7136 14324-14326 IN denotes of
T7137 14327-14332 CD denotes GATA3
T7138 14333-14337 VBD denotes were
T7139 14338-14345 VBN denotes induced
T7140 14346-14350 RB denotes also
T7141 14351-14353 IN denotes in
T7142 14354-14357 DT denotes the
T7143 14358-14365 NN denotes absence
T7144 14366-14368 IN denotes of
T7145 14369-14373 NN denotes IL-4
T7146 14373-14374 , denotes ,
T7147 14375-14377 IN denotes as
T7148 14378-14380 PRP denotes it
T7149 14381-14384 VBD denotes was
T7150 14385-14395 RB denotes previously
T7151 14396-14404 VBN denotes observed
T7152 14405-14406 NNP denotes [
T7153 14406-14407 CD denotes 3
T7154 14407-14408 NNP denotes ]
T7155 14408-14409 . denotes .
T7156 14410-14417 RB denotes However
T7157 14417-14418 , denotes ,
T7158 14419-14421 IN denotes at
T7159 14422-14425 JJ denotes low
T7160 14426-14439 NN denotes concentration
T7161 14439-14440 , denotes ,
T7162 14441-14445 NN denotes IL-4
T7163 14446-14449 VBD denotes was
T7164 14450-14454 JJ denotes able
T7165 14455-14457 TO denotes to
T7166 14458-14468 RB denotes marginally
T7167 14469-14475 VB denotes induce
T7168 14476-14481 CD denotes FOXP3
T7169 14482-14492 NN denotes expression
T7170 14492-14493 . denotes .
T7171 14494-14496 IN denotes Of
T7172 14497-14501 NN denotes note
T7173 14501-14502 , denotes ,
T7174 14503-14508 NNP denotes GATA3
T7175 14509-14512 VBD denotes was
T7176 14513-14517 RB denotes also
T7177 14518-14525 VBN denotes induced
T7178 14526-14528 IN denotes in
T7179 14529-14532 DT denotes the
T7180 14533-14541 NN denotes presence
T7181 14542-14544 IN denotes of
T7182 14545-14550 NNP denotes TGF-β
T7183 14551-14553 IN denotes at
T7184 14554-14558 JJ denotes high
T7185 14559-14563 NN denotes IL-4
T7186 14564-14577 NN denotes concentration
T7187 14578-14579 -LRB- denotes (
T7188 14579-14585 NN denotes Figure
T7189 14586-14588 NN denotes 5A
T7190 14589-14592 CC denotes and
T7191 14593-14595 NN denotes 5B
T7192 14595-14596 -RRB- denotes )
T7193 14596-14597 . denotes .
T7194 14598-14601 DT denotes The
T7195 14602-14606 NN denotes IL-4
T7196 14607-14615 VBN denotes mediated
T7197 14616-14626 NN denotes prevention
T7198 14627-14629 IN denotes of
T7199 14630-14635 CD denotes FOXP3
T7200 14636-14646 NN denotes expression
T7201 14647-14650 VBD denotes was
T7202 14651-14654 RB denotes not
T7203 14655-14661 VBN denotes caused
T7204 14662-14664 IN denotes by
T7205 14665-14678 NNS denotes interferences
T7206 14679-14681 IN denotes of
T7207 14682-14685 DT denotes the
T7208 14686-14694 NN denotes receptor
T7209 14695-14704 VBG denotes signaling
T7210 14704-14705 , denotes ,
T7211 14706-14713 IN denotes because
T7212 14714-14717 DT denotes the
T7213 14718-14733 NN denotes phosphorylation
T7214 14734-14736 IN denotes of
T7215 14737-14742 CD denotes SMAD2
T7216 14743-14745 CC denotes or
T7217 14746-14751 CD denotes STAT6
T7218 14752-14755 VBD denotes was
T7219 14756-14759 RB denotes not
T7220 14760-14768 VBN denotes affected
T7221 14769-14771 IN denotes by
T7222 14772-14775 DT denotes the
T7223 14776-14784 NN denotes addition
T7224 14785-14787 IN denotes of
T7225 14788-14792 NN denotes IL-4
T7226 14793-14799 CC denotes and/or
T7227 14800-14805 NN denotes TGF-β
T7228 14805-14806 , denotes ,
T7229 14807-14812 WDT denotes which
T7230 14813-14825 VBZ denotes demonstrates
T7231 14826-14830 IN denotes that
T7232 14831-14835 NN denotes IL-4
T7233 14836-14838 RB denotes as
T7234 14839-14843 RB denotes well
T7235 14844-14846 IN denotes as
T7236 14847-14852 JJ denotes TGF-β
T7237 14853-14862 VBG denotes signaling
T7238 14863-14867 VBD denotes were
T7239 14868-14878 JJ denotes functional
T7240 14879-14884 IN denotes under
T7241 14885-14890 DT denotes these
T7242 14891-14901 NNS denotes conditions
T7243 14902-14903 -LRB- denotes (
T7244 14903-14909 NN denotes Figure
T7245 14910-14912 NN denotes 5C
T7246 14912-14913 -RRB- denotes )
T7247 14913-14914 . denotes .
T7248 14915-14925 VBG denotes Increasing
T7249 14926-14933 NNS denotes amounts
T7250 14934-14936 IN denotes of
T7251 14937-14941 NN denotes IL-4
T7252 14942-14950 NN denotes increase
T7253 14951-14964 JJ denotes intracellular
T7254 14965-14970 NNP denotes GATA3
T7255 14970-14971 , denotes ,
T7256 14972-14979 IN denotes whereas
T7257 14980-14985 CD denotes FOXP3
T7258 14986-14995 VBD denotes decreased
T7259 14995-14996 , denotes ,
T7260 14997-15002 WDT denotes which
T7261 15003-15005 VBZ denotes is
T7262 15006-15016 JJ denotes consistent
T7263 15017-15021 IN denotes with
T7264 15022-15025 DT denotes the
T7265 15026-15030 NNP denotes mRNA
T7266 15031-15039 NN denotes analysis
T7267 15040-15041 -LRB- denotes (
T7268 15041-15047 NN denotes Figure
T7269 15048-15050 CD denotes 5D
T7270 15050-15051 -RRB- denotes )
T7271 15051-15052 . denotes .
T7272 15053-15064 RB denotes Furthermore
T7273 15064-15065 , denotes ,
T7274 15066-15075 NN denotes injection
T7275 15076-15078 IN denotes of
T7276 15079-15083 NN denotes IL-4
T7277 15084-15088 IN denotes into
T7278 15089-15098 JJ denotes wild-type
T7279 15099-15101 NNP denotes B6
T7280 15102-15106 NNS denotes mice
T7281 15107-15116 VBD denotes decreased
T7282 15117-15120 DT denotes the
T7283 15121-15130 JJ denotes inducible
T7284 15131-15133 CC denotes or
T7285 15134-15141 JJ denotes natural
T7286 15142-15146 NNP denotes Treg
T7287 15147-15153 NN denotes number
T7288 15154-15156 IN denotes in
T7289 15157-15161 NN denotes vivo
T7290 15161-15162 . denotes .
T7291 15163-15164 DT denotes A
T7292 15165-15176 NN denotes distinction
T7293 15177-15179 IN denotes of
T7294 15180-15183 DT denotes the
T7295 15184-15188 NNP denotes Treg
T7296 15189-15196 NNS denotes subsets
T7297 15197-15199 VBZ denotes is
T7298 15200-15203 RB denotes not
T7299 15204-15212 JJ denotes possible
T7300 15212-15213 , denotes ,
T7301 15214-15221 IN denotes because
T7302 15222-15230 RB denotes recently
T7303 15231-15240 VBN denotes activated
T7304 15241-15246 NNP denotes iTreg
T7305 15247-15252 NNS denotes cells
T7306 15253-15257 RB denotes also
T7307 15258-15265 VBP denotes express
T7308 15266-15273 NN denotes surface
T7309 15274-15278 NNS denotes CD25
T7310 15278-15279 . denotes .
T7311 15280-15282 PRP denotes We
T7312 15283-15287 VBD denotes used
T7313 15288-15297 NNS denotes complexes
T7314 15298-15300 IN denotes of
T7315 15301-15312 JJ denotes recombinant
T7316 15313-15318 NN denotes mouse
T7317 15319-15323 NN denotes IL-4
T7318 15324-15325 -LRB- denotes (
T7319 15325-15331 NN denotes rmIL-4
T7320 15331-15332 -RRB- denotes )
T7321 15333-15337 CC denotes plus
T7322 15338-15347 JJ denotes anti-IL-4
T7323 15348-15358 JJ denotes monoclonal
T7324 15359-15369 NNS denotes antibodies
T7325 15370-15371 -LRB- denotes (
T7326 15371-15375 NNS denotes mAbs
T7327 15375-15376 -RRB- denotes )
T7328 15376-15377 , denotes ,
T7329 15378-15383 WDT denotes which
T7330 15384-15388 VBP denotes have
T7331 15389-15393 VBN denotes been
T7332 15394-15399 VBN denotes shown
T7333 15400-15402 TO denotes to
T7334 15403-15415 RB denotes dramatically
T7335 15416-15424 VB denotes increase
T7336 15425-15428 DT denotes the
T7337 15429-15436 NN denotes potency
T7338 15437-15439 IN denotes of
T7339 15440-15443 DT denotes the
T7340 15444-15452 NN denotes cytokine
T7341 15453-15455 IN denotes in
T7342 15456-15460 NN denotes vivo
T7343 15461-15462 NNP denotes [
T7344 15462-15464 CD denotes 19
T7345 15464-15465 NNP denotes ]
T7346 15465-15466 . denotes .
T7347 15467-15469 IN denotes In
T7348 15470-15475 DT denotes these
T7349 15476-15480 NNS denotes mice
T7350 15480-15481 , denotes ,
T7351 15482-15485 DT denotes the
T7352 15486-15496 NN denotes percentage
T7353 15497-15499 IN denotes of
T7354 15500-15503 NNP denotes CD4
T7355 15503-15504 NNP denotes +
T7356 15504-15508 NNP denotes CD25
T7357 15508-15509 NN denotes +
T7358 15510-15513 CC denotes and
T7359 15514-15519 CD denotes FOXP3
T7360 15519-15520 CD denotes +
T7361 15521-15522 NNP denotes T
T7362 15523-15528 NNS denotes cells
T7363 15529-15541 RB denotes dramatically
T7364 15542-15551 VBD denotes decreased
T7365 15552-15556 WRB denotes when
T7366 15557-15560 DT denotes the
T7367 15561-15578 JJ denotes antibody-cytokine
T7368 15579-15585 JJ denotes immune
T7369 15586-15595 NNS denotes complexes
T7370 15596-15600 VBD denotes were
T7371 15601-15609 VBN denotes injected
T7372 15610-15611 -LRB- denotes (
T7373 15611-15617 NN denotes Figure
T7374 15618-15620 CD denotes S2
T7375 15620-15621 -RRB- denotes )
T7376 15621-15622 . denotes .
T7377 15623-15627 IN denotes Upon
T7378 15628-15642 NN denotes administration
T7379 15643-15645 IN denotes of
T7380 15646-15652 NN denotes rmIL-4
T7381 15653-15657 CC denotes plus
T7382 15658-15667 JJ denotes anti-IL-4
T7383 15668-15671 NNP denotes mAb
T7384 15672-15681 NNS denotes complexes
T7385 15681-15682 , denotes ,
T7386 15683-15686 DT denotes the
T7387 15687-15692 JJ denotes total
T7388 15693-15699 NN denotes number
T7389 15700-15702 IN denotes of
T7390 15703-15706 NNP denotes CD4
T7391 15706-15707 NNP denotes +
T7392 15707-15711 NNP denotes CD25
T7393 15711-15712 VBD denotes +
T7394 15713-15714 NNP denotes T
T7395 15715-15719 NN denotes cell
T7396 15719-15720 , denotes ,
T7397 15721-15723 RB denotes as
T7398 15724-15728 RB denotes well
T7399 15729-15731 IN denotes as
T7400 15732-15735 DT denotes the
T7401 15736-15741 JJ denotes Foxp3
T7402 15741-15742 NN denotes +
T7403 15743-15744 NNP denotes T
T7404 15745-15750 NNS denotes cells
T7405 15751-15761 VBN denotes diminished
T7406 15762-15764 IN denotes by
T7407 15765-15769 NN denotes half
T7408 15770-15771 -LRB- denotes (
T7409 15771-15777 NN denotes Figure
T7410 15778-15781 CD denotes S2G
T7411 15782-15785 CC denotes and
T7412 15786-15789 CD denotes S2H
T7413 15789-15790 -RRB- denotes )
T7414 15790-15791 , denotes ,
T7415 15792-15802 VBG denotes confirming
T7416 15803-15807 IN denotes that
T7417 15808-15811 DT denotes the
T7418 15812-15817 JJR denotes lower
T7419 15818-15828 NN denotes percentage
T7420 15829-15832 VBD denotes was
T7421 15833-15836 RB denotes not
T7422 15837-15840 JJ denotes due
T7423 15841-15843 TO denotes to
T7424 15844-15846 DT denotes an
T7425 15847-15855 NN denotes increase
T7426 15856-15858 IN denotes in
T7427 15859-15862 DT denotes the
T7428 15863-15866 NNP denotes CD4
T7429 15866-15867 NNP denotes +
T7430 15867-15871 NNP denotes CD25
T7431 15873-15878 NNS denotes cells
T7432 15878-15879 , denotes ,
T7433 15880-15883 CC denotes but
T7434 15884-15885 DT denotes a
T7435 15886-15890 JJ denotes real
T7436 15891-15899 NN denotes decrease
T7437 15900-15902 IN denotes of
T7438 15903-15906 NNP denotes CD4
T7439 15906-15907 NNP denotes +
T7440 15907-15911 NNP denotes CD25
T7441 15911-15912 VBD denotes +
T7442 15913-15914 NNP denotes T
T7443 15915-15920 NNS denotes cells
T7444 15920-15921 . denotes .
T7445 15922-15924 IN denotes In
T7446 15925-15935 NN denotes conclusion
T7447 15935-15936 , denotes ,
T7448 15937-15941 NN denotes IL-4
T7449 15942-15952 RB denotes negatively
T7450 15953-15962 VBZ denotes regulates
T7451 15963-15966 DT denotes the
T7452 15967-15974 JJ denotes natural
T7453 15975-15977 CC denotes or
T7454 15978-15987 JJ denotes inducible
T7455 15988-15992 NNP denotes Treg
T7456 15993-15997 NN denotes cell
T7457 15998-16006 NN denotes turnover
T7458 16007-16010 RB denotes not
T7459 16011-16015 RB denotes only
T7460 16016-16018 IN denotes in
T7461 16019-16024 NN denotes vitro
T7462 16025-16028 CC denotes but
T7463 16029-16033 RB denotes also
T7464 16034-16036 IN denotes in
T7465 16037-16041 NN denotes vivo
T7466 16041-16042 . denotes .
T7467 16043-16045 TO denotes To
T7468 16046-16051 VB denotes study
T7469 16052-16055 DT denotes the
T7470 16056-16063 NNS denotes effects
T7471 16064-16066 IN denotes of
T7472 16067-16071 NN denotes IL-4
T7473 16072-16074 IN denotes on
T7474 16075-16091 JJ denotes already-existing
T7475 16092-16097 JJ denotes human
T7476 16098-16105 JJ denotes natural
T7477 16106-16108 CC denotes or
T7478 16109-16118 JJ denotes inducible
T7479 16119-16123 NNP denotes Treg
T7480 16124-16129 NNS denotes cells
T7481 16129-16130 , denotes ,
T7482 16131-16133 PRP denotes we
T7483 16134-16141 VBD denotes exposed
T7484 16142-16148 VBN denotes sorted
T7485 16149-16153 NNP denotes CD25
T7486 16153-16154 CD denotes +
T7487 16155-16156 NNP denotes T
T7488 16157-16162 NNS denotes cells
T7489 16163-16164 -LRB- denotes (
T7490 16164-16169 JJ denotes nTreg
T7491 16170-16175 NNS denotes cells
T7492 16175-16176 -RRB- denotes )
T7493 16177-16179 TO denotes to
T7494 16180-16184 NN denotes IL-4
T7495 16185-16188 CC denotes and
T7496 16189-16197 VBD denotes analyzed
T7497 16198-16203 CD denotes FOXP3
T7498 16204-16214 NN denotes expression
T7499 16215-16218 CC denotes and
T7500 16219-16230 JJ denotes suppressive
T7501 16231-16239 NN denotes capacity
T7502 16239-16240 . denotes .
T7503 16241-16243 IN denotes In
T7504 16244-16260 JJ denotes already-existing
T7505 16261-16265 NNP denotes Treg
T7506 16266-16271 NNS denotes cells
T7507 16271-16272 , denotes ,
T7508 16273-16277 NN denotes IL-4
T7509 16278-16284 VBD denotes failed
T7510 16285-16287 TO denotes to
T7511 16288-16295 VB denotes inhibit
T7512 16296-16301 CD denotes FOXP3
T7513 16302-16312 NN denotes expression
T7514 16313-16314 -LRB- denotes (
T7515 16314-16320 NN denotes Figure
T7516 16321-16324 CD denotes S3A
T7517 16324-16325 -RRB- denotes )
T7518 16325-16326 , denotes ,
T7519 16327-16330 CC denotes and
T7520 16331-16334 DT denotes the
T7521 16335-16346 JJ denotes suppressive
T7522 16347-16355 NN denotes capacity
T7523 16356-16359 VBD denotes was
T7524 16360-16363 RB denotes not
T7525 16364-16371 VBN denotes altered
T7526 16372-16373 -LRB- denotes (
T7527 16373-16379 NN denotes Figure
T7528 16380-16383 CD denotes S3C
T7529 16383-16384 -RRB- denotes )
T7530 16384-16385 . denotes .
T7531 16386-16395 RB denotes Similarly
T7532 16396-16408 JJ denotes pre-existing
T7533 16409-16414 NNP denotes iTreg
T7534 16415-16420 NNS denotes cells
T7535 16421-16424 VBD denotes did
T7536 16425-16428 RB denotes not
T7537 16429-16437 VB denotes decrease
T7538 16438-16443 CD denotes FOXP3
T7539 16444-16454 NN denotes expression
T7540 16455-16459 IN denotes upon
T7541 16460-16464 NN denotes IL-4
T7542 16465-16473 NN denotes exposure
T7543 16474-16475 -LRB- denotes (
T7544 16475-16481 NN denotes Figure
T7545 16482-16485 CD denotes S3B
T7546 16485-16486 -RRB- denotes )
T7547 16486-16487 . denotes .
T8764 17545-17548 DT denotes The
T8765 17549-17553 NNP denotes Role
T8766 17554-17556 IN denotes of
T8767 17557-17562 NNP denotes TGF-β
T8768 17563-17565 IN denotes in
T8769 17566-17567 NNP denotes T
T8770 17568-17572 NNP denotes Cell
T8771 17573-17588 NNP denotes Differentiation
T8772 17589-17597 IN denotes Although
T8773 17598-17603 NNP denotes TGF-β
T8774 17604-17611 VBD denotes reduced
T8775 17612-17616 NNP denotes CD25
T8776 17616-17617 , denotes ,
T8777 17618-17622 NN denotes IL-4
T8778 17623-17633 NN denotes expression
T8779 17633-17634 , denotes ,
T8780 17635-17638 CC denotes and
T8781 17639-17643 NNP denotes CD25
T8782 17644-17654 NN denotes expression
T8783 17655-17656 -LRB- denotes (
T8784 17656-17662 NN denotes Figure
T8785 17663-17665 NN denotes 6B
T8786 17665-17666 -RRB- denotes )
T8787 17666-17667 , denotes ,
T8788 17668-17672 NN denotes IL-4
T8789 17673-17686 RB denotes significantly
T8790 17687-17696 VBD denotes inhibited
T8791 17697-17702 NNP denotes TGF-β
T8792 17703-17711 VBD denotes mediated
T8793 17712-17721 NN denotes induction
T8794 17722-17724 IN denotes of
T8795 17725-17730 CD denotes FOXP3
T8796 17731-17733 IN denotes in
T8797 17734-17739 JJ denotes naive
T8798 17740-17741 NNP denotes T
T8799 17742-17747 NNS denotes cells
T8800 17748-17754 VBN denotes driven
T8801 17755-17761 IN denotes toward
T8802 17762-17767 CD denotes FOXP3
T8803 17767-17768 CD denotes +
T8804 17769-17770 NNP denotes T
T8805 17771-17776 NNS denotes cells
T8806 17776-17777 , denotes ,
T8807 17778-17780 IN denotes as
T8808 17781-17786 VBN denotes shown
T8809 17787-17789 IN denotes by
T8810 17790-17794 NNP denotes FACS
T8811 17795-17803 NN denotes analysis
T8812 17804-17805 -LRB- denotes (
T8813 17805-17811 NN denotes Figure
T8814 17812-17814 NN denotes 6A
T8815 17814-17815 -RRB- denotes )
T8816 17815-17816 . denotes .
T8817 17817-17819 PRP denotes It
T8818 17820-17822 VBZ denotes is
T8819 17823-17828 VBN denotes known
T8820 17829-17833 IN denotes that
T8821 17834-17838 NN denotes IL-4
T8822 17839-17841 VBZ denotes is
T8823 17842-17843 DT denotes a
T8824 17844-17850 JJ denotes potent
T8825 17851-17857 NN denotes growth
T8826 17858-17864 NN denotes factor
T8827 17865-17868 CC denotes and
T8828 17869-17872 MD denotes may
T8829 17873-17882 RB denotes therefore
T8830 17883-17888 VB denotes favor
T8831 17889-17892 DT denotes the
T8832 17893-17906 NN denotes proliferation
T8833 17907-17909 IN denotes of
T8834 17910-17915 CD denotes FOXP3
T8835 17917-17922 NNS denotes cells
T8836 17923-17926 CC denotes and
T8837 17927-17931 RB denotes thus
T8838 17932-17940 VB denotes decrease
T8839 17941-17944 DT denotes the
T8840 17945-17953 JJ denotes relative
T8841 17954-17964 NN denotes percentage
T8842 17965-17967 IN denotes of
T8843 17968-17973 CD denotes FOXP3
T8844 17973-17974 CD denotes +
T8845 17975-17980 NNS denotes cells
T8846 17980-17981 . denotes .
T8847 17982-17989 RB denotes However
T8848 17989-17990 , denotes ,
T8849 17991-17999 NN denotes analysis
T8850 18000-18002 IN denotes of
T8851 18003-18007 NN denotes cell
T8852 18008-18016 NN denotes division
T8853 18017-18025 NNS denotes kinetics
T8854 18026-18028 IN denotes by
T8855 18029-18042 JJ denotes CFSE-labeling
T8856 18043-18055 VBD denotes demonstrated
T8857 18056-18060 IN denotes that
T8858 18061-18065 NN denotes IL-4
T8859 18066-18069 VBD denotes did
T8860 18070-18073 RB denotes not
T8861 18074-18088 RB denotes differentially
T8862 18089-18096 VB denotes promote
T8863 18097-18101 NN denotes cell
T8864 18102-18108 NN denotes growth
T8865 18109-18111 IN denotes of
T8866 18112-18117 CD denotes FOXP3
T8867 18117-18118 NN denotes +
T8868 18119-18123 IN denotes over
T8869 18124-18128 DT denotes that
T8870 18129-18131 IN denotes of
T8871 18132-18137 CD denotes FOXP3
T8872 18138-18139 . denotes .
T8873 18140-18142 IN denotes In
T8874 18143-18147 NN denotes fact
T8875 18148-18152 DT denotes both
T8876 18153-18164 NNS denotes populations
T8877 18165-18171 VBD denotes showed
T8878 18172-18181 RB denotes similarly
T8879 18182-18190 JJ denotes enhanced
T8880 18191-18204 NN denotes proliferation
T8881 18205-18206 -LRB- denotes (
T8882 18206-18212 NN denotes Figure
T8883 18213-18215 NN denotes 6A
T8884 18215-18216 -RRB- denotes )
T8885 18216-18217 . denotes .
T8886 18218-18229 RB denotes Furthermore
T8887 18230-18233 DT denotes the
T8888 18234-18239 NNP denotes TGF-β
T8889 18240-18248 VBD denotes mediated
T8890 18249-18258 NN denotes induction
T8891 18259-18261 IN denotes of
T8892 18262-18267 CD denotes FOXP3
T8893 18268-18278 NN denotes expression
T8894 18279-18282 VBD denotes was
T8895 18283-18286 RB denotes not
T8896 18287-18293 VBN denotes caused
T8897 18294-18296 IN denotes by
T8898 18297-18307 NN denotes overgrowth
T8899 18308-18310 IN denotes of
T8900 18311-18312 DT denotes a
T8901 18313-18317 NNP denotes CD25
T8902 18318-18323 CD denotes FOXP3
T8903 18323-18324 NN denotes +
T8904 18325-18333 NN denotes minority
T8905 18333-18334 , denotes ,
T8906 18335-18340 IN denotes since
T8907 18341-18344 DT denotes the
T8908 18345-18351 NN denotes number
T8909 18352-18354 IN denotes of
T8910 18355-18360 CD denotes FOXP3
T8911 18360-18361 CD denotes +
T8912 18362-18367 NNS denotes cells
T8913 18368-18371 VBD denotes was
T8914 18372-18382 JJ denotes low/absent
T8915 18383-18385 IN denotes in
T8916 18386-18389 DT denotes the
T8917 18390-18398 VBN denotes purified
T8918 18399-18402 NNP denotes CD4
T8919 18402-18403 NNP denotes +
T8920 18403-18409 NNP denotes CD45RA
T8921 18409-18410 VBD denotes +
T8922 18411-18412 NNP denotes T
T8923 18413-18418 NNS denotes cells
T8924 18419-18420 -LRB- denotes (
T8925 18420-18427 IN denotes between
T8926 18428-18429 CD denotes 0
T8927 18429-18430 NN denotes %
T8928 18431-18434 CC denotes and
T8929 18435-18436 CD denotes 1
T8930 18436-18437 NN denotes %
T8931 18437-18438 -RRB- denotes )
T8932 18438-18439 , denotes ,
T8933 18440-18443 CC denotes and
T8934 18444-18447 DT denotes the
T8935 18448-18453 CD denotes FOXP3
T8936 18453-18454 NN denotes +
T8937 18455-18460 NNS denotes cells
T8938 18461-18465 VBD denotes were
T8939 18466-18469 RB denotes not
T8940 18470-18478 VBN denotes confined
T8941 18479-18481 TO denotes to
T8942 18482-18485 DT denotes the
T8943 18486-18492 RB denotes highly
T8944 18493-18500 VBN denotes divided
T8945 18501-18506 NNS denotes cells
T8946 18506-18507 . denotes .
T8947 18508-18512 CD denotes CD25
T8948 18513-18516 VBD denotes was
T8949 18517-18531 VBN denotes down-regulated
T8950 18532-18534 IN denotes in
T8951 18535-18540 NNP denotes TGF-β
T8952 18541-18548 VBD denotes treated
T8953 18549-18554 NNS denotes cells
T8954 18555-18562 VB denotes compare
T8955 18563-18565 TO denotes to
T8956 18566-18575 VBN denotes activated
T8957 18576-18577 NNP denotes T
T8958 18578-18583 NNS denotes cells
T8959 18583-18584 , denotes ,
T8960 18585-18590 WDT denotes which
T8961 18591-18594 VBD denotes was
T8962 18595-18599 RB denotes even
T8963 18600-18604 RBR denotes more
T8964 18605-18615 JJ denotes pronounced
T8965 18616-18618 IN denotes in
T8966 18619-18624 NNS denotes cells
T8967 18625-18633 VBN denotes cultured
T8968 18634-18638 IN denotes with
T8969 18639-18644 NNP denotes TGF-β
T8970 18645-18648 CC denotes and
T8971 18649-18653 NN denotes IL-4
T8972 18653-18654 . denotes .
T8973 19280-19283 DT denotes The
T8974 19284-19292 NN denotes addition
T8975 19293-19295 IN denotes of
T8976 19296-19300 NN denotes IL-4
T8977 19301-19303 TO denotes to
T8978 19304-19317 JJ denotes iTreg-driving
T8979 19318-19328 NNS denotes conditions
T8980 19329-19338 VBD denotes decreased
T8981 19339-19342 DT denotes the
T8982 19343-19349 NN denotes number
T8983 19350-19352 IN denotes of
T8984 19353-19358 CD denotes FOXP3
T8985 19358-19359 CD denotes +
T8986 19360-19365 NNS denotes cells
T8987 19366-19367 -LRB- denotes (
T8988 19367-19373 NN denotes Figure
T8989 19374-19376 NN denotes 6B
T8990 19376-19377 -RRB- denotes )
T8991 19377-19378 . denotes .
T8992 19379-19381 IN denotes In
T8993 19382-19386 NN denotes line
T8994 19387-19391 IN denotes with
T8995 19392-19395 DT denotes the
T8996 19396-19404 JJ denotes previous
T8997 19405-19413 NNS denotes findings
T8998 19413-19414 , denotes ,
T8999 19415-19418 DT denotes the
T9000 19419-19423 NN denotes IL-4
T9001 19424-19433 VBG denotes producing
T9002 19434-19439 NNS denotes cells
T9003 19440-19443 CC denotes and
T9004 19444-19447 DT denotes the
T9005 19448-19453 CD denotes FOXP3
T9006 19454-19464 VBG denotes expressing
T9007 19465-19470 NNS denotes cells
T9008 19471-19474 VBP denotes are
T9009 19475-19489 VBG denotes nonoverlapping
T9010 19490-19501 NNS denotes populations
T9011 19501-19502 . denotes .
T9012 19503-19508 IN denotes Since
T9013 19509-19514 CD denotes FOXP3
T9014 19515-19517 VBZ denotes is
T9015 19518-19523 VBN denotes known
T9016 19524-19526 TO denotes to
T9017 19527-19530 VB denotes act
T9018 19531-19533 IN denotes as
T9019 19534-19535 DT denotes a
T9020 19536-19545 NN denotes repressor
T9021 19546-19548 IN denotes of
T9022 19549-19557 NN denotes cytokine
T9023 19558-19568 NN denotes expression
T9024 19569-19570 NNP denotes [
T9025 19570-19572 CD denotes 20
T9026 19572-19573 NNP denotes ]
T9027 19573-19574 , denotes ,
T9028 19575-19577 PRP denotes we
T9029 19578-19587 RB denotes therefore
T9030 19588-19596 VBD denotes analyzed
T9031 19597-19602 NNP denotes GATA3
T9032 19603-19606 CC denotes and
T9033 19607-19612 NNP denotes FOXP3
T9034 19613-19623 NN denotes expression
T9035 19623-19624 . denotes .
T9036 19625-19628 DT denotes The
T9037 19629-19639 NN denotes expression
T9038 19640-19647 NN denotes kinetic
T9039 19648-19650 IN denotes of
T9040 19651-19656 JJ denotes naive
T9041 19657-19658 NNP denotes T
T9042 19659-19664 NNS denotes cells
T9043 19665-19672 VBN denotes exposed
T9044 19673-19675 TO denotes to
T9045 19676-19680 NN denotes IL-4
T9046 19681-19684 CC denotes and
T9047 19685-19690 JJ denotes TGF-β
T9048 19691-19703 VBD denotes demonstrated
T9049 19704-19708 IN denotes that
T9050 19709-19714 NNP denotes GATA3
T9051 19715-19718 CC denotes and
T9052 19719-19724 NNP denotes FOXP3
T9053 19725-19728 VBP denotes are
T9054 19729-19738 RB denotes initially
T9055 19739-19744 VBN denotes found
T9056 19745-19747 IN denotes in
T9057 19748-19756 JJ denotes separate
T9058 19757-19768 NNS denotes populations
T9059 19769-19770 -LRB- denotes (
T9060 19770-19773 NN denotes day
T9061 19774-19775 CD denotes 2
T9062 19775-19776 -RRB- denotes )
T9063 19776-19777 , denotes ,
T9064 19778-19781 CC denotes but
T9065 19782-19793 RB denotes transiently
T9066 19794-19804 JJ denotes co-express
T9067 19805-19809 DT denotes both
T9068 19810-19817 NNS denotes factors
T9069 19818-19819 -LRB- denotes (
T9070 19819-19823 NNS denotes days
T9071 19824-19825 CD denotes 4
T9072 19826-19827 CD denotes 8
T9073 19827-19828 -RRB- denotes )
T9074 19828-19829 , denotes ,
T9075 19830-19836 IN denotes before
T9076 19837-19849 VBG denotes establishing
T9077 19850-19858 JJ denotes separate
T9078 19859-19870 NNS denotes populations
T9079 19871-19873 IN denotes at
T9080 19874-19877 DT denotes the
T9081 19878-19881 NN denotes end
T9082 19882-19884 IN denotes of
T9083 19885-19888 DT denotes the
T9084 19889-19904 NN denotes differentiation
T9085 19905-19912 NN denotes process
T9086 19913-19914 -LRB- denotes (
T9087 19914-19917 NN denotes day
T9088 19918-19920 CD denotes 10
T9089 19920-19921 : denotes ;
T9090 19922-19928 NN denotes Figure
T9091 19929-19931 NN denotes 6C
T9092 19931-19932 -RRB- denotes )
T9093 19932-19933 , denotes ,
T9094 19934-19944 VBG denotes suggesting
T9095 19945-19949 IN denotes that
T9096 19950-19955 NNP denotes GATA3
T9097 19956-19964 VBZ denotes inhibits
T9098 19965-19968 DT denotes the
T9099 19969-19980 NN denotes development
T9100 19981-19983 IN denotes of
T9101 19984-19989 NNP denotes iTreg
T9102 19990-19995 NNS denotes cells
T9103 19996-19998 IN denotes by
T9104 19999-20009 VBG denotes repressing
T9105 20010-20015 NNP denotes FOXP3
T9106 20015-20016 . denotes .
T9107 20017-20022 DT denotes These
T9108 20023-20030 NNS denotes results
T9109 20031-20037 VBD denotes showed
T9110 20038-20042 IN denotes that
T9111 20043-20047 NN denotes IL-4
T9112 20048-20052 NNS denotes acts
T9113 20053-20055 IN denotes in
T9114 20056-20061 NN denotes vitro
T9115 20062-20064 IN denotes as
T9116 20065-20067 DT denotes an
T9117 20068-20077 NN denotes inhibitor
T9118 20078-20080 IN denotes of
T9119 20081-20086 CD denotes FOXP3
T9120 20087-20097 NN denotes expression
T9121 20097-20098 , denotes ,
T9122 20099-20106 IN denotes without
T9123 20107-20118 VBG denotes interfering
T9124 20119-20123 IN denotes with
T9125 20124-20129 JJ denotes TGF-β
T9126 20130-20139 VBG denotes signaling
T9127 20139-20140 , denotes ,
T9128 20141-20149 RB denotes probably
T9129 20150-20156 VBG denotes acting
T9130 20157-20159 IN denotes at
T9131 20160-20163 DT denotes the
T9132 20164-20169 NN denotes level
T9133 20170-20172 IN denotes of
T9134 20173-20186 NN denotes transcription
T9135 20187-20194 NNS denotes factors
T9136 20194-20195 , denotes ,
T9137 20196-20199 CC denotes and
T9138 20200-20208 RB denotes possibly
T9139 20209-20211 IN denotes by
T9140 20212-20213 DT denotes a
T9141 20214-20229 JJ denotes GATA3-dependent
T9142 20230-20239 NN denotes mechanism
T9143 20239-20240 . denotes .
T10333 20242-20247 CD denotes GATA3
T10334 20248-20250 VBZ denotes Is
T10335 20251-20252 DT denotes a
T10336 20253-20261 JJ denotes Negative
T10337 20262-20271 NNP denotes Regulator
T10338 20272-20274 IN denotes of
T10339 20275-20280 NNP denotes FOXP3
T10340 20281-20291 NNP denotes Expression
T10341 20292-20297 NNP denotes FOXP3
T10342 20298-20308 NN denotes expression
T10343 20309-20318 VBD denotes decreased
T10344 20319-20323 RB denotes once
T10345 20324-20329 JJ denotes GATA3
T10346 20330-20340 NN denotes expression
T10347 20341-20343 VBZ denotes is
T10348 20344-20348 JJ denotes high
T10349 20348-20349 : denotes ;
T10350 20350-20359 RB denotes therefore
T10351 20359-20360 , denotes ,
T10352 20361-20363 PRP denotes we
T10353 20364-20376 VBD denotes hypothesized
T10354 20377-20378 DT denotes a
T10355 20379-20388 JJ denotes potential
T10356 20389-20393 NN denotes role
T10357 20394-20397 IN denotes for
T10358 20398-20403 NNP denotes GATA3
T10359 20404-20406 IN denotes in
T10360 20407-20417 VBG denotes repressing
T10361 20418-20423 NNP denotes FOXP3
T10362 20423-20424 . denotes .
T10363 20425-20432 IN denotes Besides
T10364 20433-20438 NNP denotes GATA3
T10365 20438-20439 NNP denotes ′
T10366 20439-20440 VBZ denotes s
T10367 20441-20451 JJ denotes well-known
T10368 20452-20460 JJ denotes positive
T10369 20461-20467 NN denotes effect
T10370 20468-20470 IN denotes on
T10371 20471-20475 NN denotes gene
T10372 20476-20486 NN denotes regulation
T10373 20486-20487 , denotes ,
T10374 20488-20493 NNP denotes GATA3
T10375 20493-20494 NNP denotes ′
T10376 20494-20495 VBZ denotes s
T10377 20496-20506 JJ denotes repressive
T10378 20507-20519 NNS denotes capabilities
T10379 20520-20524 VBD denotes were
T10380 20525-20535 RB denotes previously
T10381 20536-20541 VBN denotes shown
T10382 20542-20544 TO denotes to
T10383 20545-20553 VB denotes restrict
T10384 20554-20557 JJ denotes Th1
T10385 20558-20568 NN denotes commitment
T10386 20569-20571 IN denotes by
T10387 20572-20582 VBG denotes inhibiting
T10388 20583-20588 NNP denotes STAT4
T10389 20589-20599 NN denotes expression
T10390 20600-20601 NNP denotes [
T10391 20601-20605 CD denotes 2,21
T10392 20605-20606 NNP denotes ]
T10393 20606-20607 , denotes ,
T10394 20608-20611 CC denotes and
T10395 20612-20621 RB denotes therefore
T10396 20622-20627 JJ denotes GATA3
T10397 20628-20636 VBZ denotes prevents
T10398 20637-20652 NN denotes differentiation
T10399 20653-20657 IN denotes into
T10400 20658-20661 JJ denotes Th1
T10401 20662-20667 NNS denotes cells
T10402 20667-20668 . denotes .
T10403 20669-20671 TO denotes To
T10404 20672-20683 VB denotes investigate
T10405 20684-20691 IN denotes whether
T10406 20692-20697 NNP denotes GATA3
T10407 20698-20701 MD denotes can
T10408 20702-20710 RB denotes directly
T10409 20711-20718 VB denotes inhibit
T10410 20719-20724 CD denotes FOXP3
T10411 20725-20734 NN denotes induction
T10412 20734-20735 , denotes ,
T10413 20736-20738 PRP denotes we
T10414 20739-20749 VBD denotes transduced
T10415 20750-20755 NNP denotes GATA3
T10416 20756-20758 CC denotes or
T10417 20759-20760 DT denotes a
T10418 20761-20770 VBN denotes truncated
T10419 20771-20776 NNP denotes GATA3
T10420 20777-20784 VBG denotes lacking
T10421 20785-20788 DT denotes the
T10422 20789-20800 JJ denotes DNA-binding
T10423 20801-20807 NN denotes domain
T10424 20808-20810 IN denotes in
T10425 20811-20816 JJ denotes human
T10426 20817-20824 JJ denotes primary
T10427 20825-20828 NNP denotes CD4
T10428 20828-20829 NNP denotes +
T10429 20829-20835 NNP denotes CD45RA
T10430 20835-20836 VBD denotes +
T10431 20837-20838 NNP denotes T
T10432 20839-20844 NNS denotes cells
T10433 20845-20850 VBG denotes using
T10434 20851-20852 DT denotes a
T10435 20853-20862 JJ denotes TAT-fused
T10436 20862-20863 , denotes ,
T10437 20864-20877 RB denotes recombinantly
T10438 20878-20887 VBN denotes expressed
T10439 20888-20893 NNP denotes GATA3
T10440 20893-20894 . denotes .
T10441 20895-20900 IN denotes After
T10442 20901-20913 NN denotes transduction
T10443 20913-20914 , denotes ,
T10444 20915-20918 DT denotes the
T10445 20919-20924 NNS denotes cells
T10446 20925-20929 VBD denotes were
T10447 20930-20939 VBN denotes activated
T10448 20940-20944 IN denotes with
T10449 20945-20952 JJ denotes soluble
T10450 20953-20961 JJ denotes anti-CD3
T10451 20961-20962 NN denotes /
T10452 20962-20966 CD denotes CD28
T10453 20967-20969 IN denotes in
T10454 20970-20973 DT denotes the
T10455 20974-20982 NN denotes presence
T10456 20983-20985 CC denotes or
T10457 20986-20993 NN denotes absence
T10458 20994-20996 IN denotes of
T10459 20997-21002 NNP denotes TGF-β
T10460 21002-21003 . denotes .
T10461 21004-21013 CD denotes TAT-GATA3
T10462 21014-21017 VBD denotes was
T10463 21018-21030 RB denotes successfully
T10464 21031-21041 VBN denotes transduced
T10465 21042-21044 IN denotes in
T10466 21045-21046 DT denotes a
T10467 21047-21058 JJ denotes homogeneous
T10468 21059-21062 CC denotes and
T10469 21063-21077 JJ denotes dose-dependent
T10470 21078-21084 NN denotes manner
T10471 21085-21089 IN denotes into
T10472 21090-21095 JJ denotes human
T10473 21096-21099 NNP denotes CD4
T10474 21099-21100 CD denotes +
T10475 21101-21102 NNP denotes T
T10476 21103-21108 NNS denotes cells
T10477 21109-21110 -LRB- denotes (
T10478 21110-21116 NN denotes Figure
T10479 21117-21119 NN denotes 7A
T10480 21119-21120 , denotes ,
T10481 21121-21126 JJ denotes upper
T10482 21127-21132 NN denotes panel
T10483 21132-21133 -RRB- denotes )
T10484 21133-21134 . denotes .
T10485 21135-21144 CD denotes TAT-GATA3
T10486 21145-21152 VBN denotes reduced
T10487 21153-21158 CD denotes FOXP3
T10488 21159-21169 NN denotes expression
T10489 21170-21172 IN denotes in
T10490 21173-21174 DT denotes a
T10491 21175-21189 JJ denotes dose-dependent
T10492 21190-21196 NN denotes manner
T10493 21196-21197 , denotes ,
T10494 21198-21205 IN denotes whereas
T10495 21206-21207 DT denotes a
T10496 21208-21219 JJ denotes DNA-binding
T10497 21220-21226 NN denotes domain
T10498 21227-21236 VBN denotes truncated
T10499 21237-21244 NN denotes version
T10500 21245-21246 -LRB- denotes (
T10501 21246-21260 CD denotes TAT-ΔDBD-GATA3
T10502 21260-21261 -RRB- denotes )
T10503 21262-21265 VBD denotes did
T10504 21266-21269 RB denotes not
T10505 21270-21276 VB denotes affect
T10506 21277-21282 CD denotes FOXP3
T10507 21283-21293 NN denotes expression
T10508 21294-21296 IN denotes as
T10509 21297-21305 VBN denotes compared
T10510 21306-21310 IN denotes with
T10511 21311-21321 NN denotes expression
T10512 21322-21324 IN denotes in
T10513 21325-21337 JJ denotes untransduced
T10514 21338-21343 NNS denotes cells
T10515 21344-21345 -LRB- denotes (
T10516 21345-21351 NN denotes Figure
T10517 21352-21354 NN denotes 7A
T10518 21354-21355 -RRB- denotes )
T10519 21355-21356 . denotes .
T10520 21357-21359 IN denotes In
T10521 21360-21368 NN denotes addition
T10522 21368-21369 , denotes ,
T10523 21370-21372 PRP denotes we
T10524 21373-21381 VBD denotes analyzed
T10525 21382-21385 DT denotes the
T10526 21386-21396 JJ denotes inhibitory
T10527 21397-21403 NN denotes effect
T10528 21404-21406 IN denotes of
T10529 21407-21412 CD denotes GATA3
T10530 21413-21415 IN denotes on
T10531 21416-21421 CD denotes FOXP3
T10532 21422-21424 IN denotes in
T10533 21425-21435 JJ denotes transgenic
T10534 21436-21440 NNP denotes DO11
T10535 21440-21443 CD denotes .10
T10536 21444-21448 NNS denotes mice
T10537 21448-21449 , denotes ,
T10538 21450-21464 RB denotes constitutively
T10539 21465-21479 VBG denotes overexpressing
T10540 21480-21485 NNP denotes GATA3
T10541 21486-21491 IN denotes under
T10542 21492-21495 DT denotes the
T10543 21496-21503 NN denotes control
T10544 21504-21506 IN denotes of
T10545 21507-21510 DT denotes the
T10546 21511-21514 NNP denotes CD2
T10547 21515-21520 NN denotes locus
T10548 21521-21528 NN denotes control
T10549 21529-21535 NN denotes region
T10550 21536-21537 -LRB- denotes (
T10551 21537-21554 JJ denotes DO11.10xCD2-GATA3
T10552 21554-21555 -RRB- denotes )
T10553 21555-21556 . denotes .
T10554 21557-21560 DT denotes The
T10555 21561-21567 JJ denotes thymic
T10556 21568-21577 NN denotes selection
T10557 21578-21582 IN denotes into
T10558 21583-21586 DT denotes the
T10559 21587-21590 NNP denotes CD4
T10560 21591-21598 NN denotes lineage
T10561 21599-21601 VBZ denotes is
T10562 21602-21609 RB denotes largely
T10563 21610-21616 JJ denotes intact
T10564 21617-21619 IN denotes in
T10565 21620-21637 NNP denotes DO11.10xCD2-GATA3
T10566 21638-21639 -LRB- denotes (
T10567 21639-21641 NNP denotes RW
T10568 21642-21650 NNPS denotes Hendriks
T10569 21650-21651 , denotes ,
T10570 21652-21663 JJ denotes unpublished
T10571 21664-21668 NNS denotes data
T10572 21668-21669 -RRB- denotes )
T10573 21669-21670 . denotes .
T10574 21671-21676 DT denotes These
T10575 21677-21681 NNS denotes mice
T10576 21682-21689 VBP denotes develop
T10577 21690-21699 NNS denotes lymphomas
T10578 21700-21702 IN denotes at
T10579 21703-21705 DT denotes an
T10580 21706-21711 JJR denotes older
T10581 21712-21715 NN denotes age
T10582 21715-21716 , denotes ,
T10583 21717-21720 CC denotes but
T10584 21721-21726 NNS denotes signs
T10585 21727-21729 IN denotes of
T10586 21730-21740 JJ denotes autoimmune
T10587 21741-21748 NN denotes disease
T10588 21749-21753 VBD denotes were
T10589 21754-21757 RB denotes not
T10590 21758-21767 VBN denotes described
T10591 21768-21769 NNP denotes [
T10592 21769-21771 CD denotes 22
T10593 21771-21772 NNP denotes ]
T10594 21772-21773 . denotes .
T10595 21774-21776 TO denotes To
T10596 21777-21788 VB denotes investigate
T10597 21789-21792 DT denotes the
T10598 21793-21799 NN denotes effect
T10599 21800-21802 IN denotes of
T10600 21803-21808 NNP denotes GATA3
T10601 21809-21811 IN denotes on
T10602 21812-21817 NNP denotes iTreg
T10603 21817-21818 , denotes ,
T10604 21819-21822 NNP denotes CD4
T10605 21822-21823 NNP denotes +
T10606 21823-21828 NNP denotes CD62L
T10607 21828-21829 NNP denotes +
T10608 21829-21833 NNP denotes CD25
T10609 21835-21840 NNS denotes cells
T10610 21841-21845 VBD denotes were
T10611 21846-21854 VBN denotes isolated
T10612 21854-21855 , denotes ,
T10613 21856-21865 VBN denotes activated
T10614 21866-21870 IN denotes with
T10615 21871-21874 NNP denotes OVA
T10616 21875-21877 IN denotes in
T10617 21878-21881 DT denotes the
T10618 21882-21890 NN denotes presence
T10619 21891-21893 CC denotes or
T10620 21894-21901 NN denotes absence
T10621 21902-21904 IN denotes of
T10622 21905-21910 NNP denotes TGF-β
T10623 21910-21911 , denotes ,
T10624 21912-21915 CC denotes and
T10625 21916-21921 JJ denotes Foxp3
T10626 21922-21932 NN denotes expression
T10627 21933-21936 VBD denotes was
T10628 21937-21945 VBN denotes analyzed
T10629 21946-21951 IN denotes after
T10630 21952-21953 CD denotes 4
T10631 21954-21955 LS denotes d
T10632 21955-21956 . denotes .
T10633 21957-21960 DT denotes The
T10634 21961-21966 JJ denotes naive
T10635 21967-21970 NNP denotes CD4
T10636 21970-21971 NNP denotes +
T10637 21971-21975 NNP denotes CD25
T10638 21977-21982 NNS denotes cells
T10639 21983-21987 VBD denotes were
T10640 21988-21993 JJ denotes Foxp3
T10641 21995-21996 -LRB- denotes (
T10642 21996-22007 JJ denotes unpublished
T10643 22008-22012 NNS denotes data
T10644 22012-22013 -RRB- denotes )
T10645 22013-22014 . denotes .
T10646 22015-22017 IN denotes As
T10647 22018-22027 VBN denotes described
T10648 22028-22031 IN denotes for
T10649 22032-22035 DT denotes the
T10650 22036-22041 JJ denotes human
T10651 22042-22047 NNS denotes cells
T10652 22047-22048 , denotes ,
T10653 22049-22054 JJ denotes TGF-β
T10654 22055-22067 RB denotes dramatically
T10655 22068-22080 JJ denotes up-regulated
T10656 22081-22086 NN denotes Foxp3
T10657 22087-22089 IN denotes in
T10658 22090-22093 DT denotes the
T10659 22094-22098 NNP denotes DO11
T10660 22098-22101 CD denotes .10
T10661 22102-22112 NN denotes littermate
T10662 22113-22120 NN denotes control
T10663 22121-22125 NNS denotes mice
T10664 22125-22126 . denotes .
T10665 22127-22129 IN denotes In
T10666 22130-22138 NN denotes contrast
T10667 22138-22139 , denotes ,
T10668 22140-22145 NNS denotes cells
T10669 22146-22150 IN denotes from
T10670 22151-22154 DT denotes the
T10671 22155-22169 NNP denotes CD2-GATA3xDO11
T10672 22169-22172 CD denotes .10
T10673 22173-22177 NNS denotes mice
T10674 22178-22184 VBD denotes showed
T10675 22185-22197 RB denotes dramatically
T10676 22198-22205 VBN denotes reduced
T10677 22206-22211 JJ denotes Foxp3
T10678 22212-22222 NN denotes expression
T10679 22223-22227 WRB denotes when
T10680 22228-22237 VBN denotes activated
T10681 22238-22242 IN denotes with
T10682 22243-22248 NNP denotes TGF-β
T10683 22249-22252 CC denotes and
T10684 22253-22256 NNP denotes OVA
T10685 22257-22258 -LRB- denotes (
T10686 22258-22264 NNP denotes Figure
T10687 22265-22267 NN denotes 7B
T10688 22267-22268 -RRB- denotes )
T10689 22268-22269 . denotes .
T10690 22270-22273 DT denotes All
T10691 22274-22278 NNS denotes mice
T10692 22279-22287 VBD denotes produced
T10693 22288-22295 JJ denotes similar
T10694 22296-22303 NNS denotes amounts
T10695 22304-22306 IN denotes of
T10696 22307-22312 NNP denotes TGF-β
T10697 22312-22313 : denotes ;
T10698 22314-22316 IN denotes in
T10699 22317-22325 NN denotes addition
T10700 22325-22326 , denotes ,
T10701 22327-22332 NNP denotes Smad7
T10702 22333-22336 VBD denotes was
T10703 22337-22344 RB denotes equally
T10704 22345-22354 VBN denotes expressed
T10705 22355-22356 NNP denotes [
T10706 22356-22358 CD denotes 23
T10707 22358-22359 NNP denotes ]
T10708 22360-22362 IN denotes in
T10709 22363-22364 NNP denotes T
T10710 22365-22370 NNS denotes cells
T10711 22371-22373 IN denotes of
T10712 22374-22378 DT denotes both
T10713 22379-22383 NNS denotes mice
T10714 22384-22391 NNS denotes strains
T10715 22392-22393 -LRB- denotes (
T10716 22393-22399 NN denotes Figure
T10717 22400-22402 NN denotes 7C
T10718 22402-22403 -RRB- denotes )
T10719 22403-22404 , denotes ,
T10720 22405-22415 VBG denotes indicating
T10721 22416-22422 JJ denotes intact
T10722 22423-22428 JJ denotes TGF-β
T10723 22429-22438 VBG denotes signaling
T10724 22438-22439 . denotes .
T10725 23318-23323 VBN denotes Taken
T10726 23324-23332 RB denotes together
T10727 23332-23333 , denotes ,
T10728 23334-23339 DT denotes these
T10729 23340-23347 NNS denotes results
T10730 23348-23360 VBD denotes demonstrated
T10731 23361-23362 DT denotes a
T10732 23363-23373 JJ denotes repressive
T10733 23374-23378 NN denotes role
T10734 23379-23381 IN denotes of
T10735 23382-23394 JJ denotes IL-4-induced
T10736 23395-23400 NNP denotes GATA3
T10737 23401-23414 NN denotes transcription
T10738 23415-23421 NN denotes factor
T10739 23422-23424 IN denotes in
T10740 23425-23428 DT denotes the
T10741 23429-23439 NN denotes generation
T10742 23440-23442 IN denotes of
T10743 23443-23448 NNP denotes iTreg
T10744 23449-23454 NNS denotes cells
T10745 23454-23455 . denotes .
T12080 23457-23462 CD denotes GATA3
T12081 23463-23472 NNPS denotes Represses
T12082 23473-23476 DT denotes the
T12083 23477-23482 NNP denotes FOXP3
T12084 23483-23491 NNP denotes Promoter
T12085 23492-23494 TO denotes To
T12086 23495-23506 VB denotes investigate
T12087 23507-23510 DT denotes the
T12088 23511-23520 JJ denotes molecular
T12089 23521-23530 NN denotes mechanism
T12090 23531-23533 IN denotes of
T12091 23534-23548 JJ denotes GATA3-mediated
T12092 23549-23559 NN denotes repression
T12093 23560-23562 IN denotes of
T12094 23563-23568 JJ denotes human
T12095 23569-23574 NNP denotes FOXP3
T12096 23574-23575 , denotes ,
T12097 23576-23579 DT denotes the
T12098 23580-23585 JJ denotes human
T12099 23586-23591 CD denotes FOXP3
T12100 23592-23600 NN denotes promoter
T12101 23601-23604 VBD denotes was
T12102 23605-23612 VBN denotes studied
T12103 23613-23616 CC denotes and
T12104 23617-23618 DT denotes a
T12105 23619-23630 JJ denotes palindromic
T12106 23631-23638 JJ denotes binding
T12107 23639-23643 NN denotes site
T12108 23644-23647 IN denotes for
T12109 23648-23653 NNP denotes GATA3
T12110 23654-23657 VBD denotes was
T12111 23658-23668 VBN denotes discovered
T12112 23668-23669 . denotes .
T12113 23670-23673 DT denotes The
T12114 23674-23686 JJ denotes GATA-binding
T12115 23687-23691 NN denotes site
T12116 23692-23694 VBZ denotes is
T12117 23695-23702 VBN denotes located
T12118 23703-23706 CD denotes 303
T12119 23707-23709 NN denotes bp
T12120 23710-23718 RB denotes upstream
T12121 23719-23723 IN denotes from
T12122 23724-23727 DT denotes the
T12123 23728-23741 NN denotes transcription
T12124 23742-23747 VB denotes start
T12125 23748-23752 NN denotes site
T12126 23753-23754 -LRB- denotes (
T12127 23754-23757 NNP denotes TSS
T12128 23757-23758 -RRB- denotes )
T12129 23759-23760 NNP denotes [
T12130 23760-23762 CD denotes 24
T12131 23762-23763 NNP denotes ]
T12132 23763-23764 . denotes .
T12133 23765-23769 DT denotes This
T12134 23770-23774 NN denotes site
T12135 23775-23777 VBZ denotes is
T12136 23778-23784 RB denotes highly
T12137 23785-23794 VBN denotes conserved
T12138 23795-23802 IN denotes between
T12139 23803-23809 NNS denotes humans
T12140 23809-23810 , denotes ,
T12141 23811-23815 NNS denotes mice
T12142 23815-23816 , denotes ,
T12143 23817-23820 CC denotes and
T12144 23821-23825 NNS denotes rats
T12145 23826-23827 -LRB- denotes (
T12146 23827-23833 NN denotes Figure
T12147 23834-23836 CD denotes S4
T12148 23836-23837 -RRB- denotes )
T12149 23838-23841 CC denotes and
T12150 23842-23845 MD denotes may
T12151 23846-23855 RB denotes therefore
T12152 23856-23860 VB denotes play
T12153 23861-23863 DT denotes an
T12154 23864-23873 JJ denotes important
T12155 23874-23878 NN denotes role
T12156 23879-23881 IN denotes in
T12157 23882-23887 CD denotes FOXP3
T12158 23888-23898 NN denotes regulation
T12159 23898-23899 . denotes .
T12160 23900-23903 DT denotes The
T12161 23904-23914 JJ denotes functional
T12162 23915-23924 NN denotes relevance
T12163 23925-23927 IN denotes of
T12164 23928-23932 DT denotes this
T12165 23933-23937 NN denotes site
T12166 23938-23941 VBD denotes was
T12167 23942-23949 VBN denotes studied
T12168 23950-23955 VBG denotes using
T12169 23956-23957 DT denotes a
T12170 23958-23972 NN denotes FOXP3-promoter
T12171 23973-23982 VB denotes construct
T12172 23983-23984 NNP denotes [
T12173 23984-23986 CD denotes 24
T12174 23986-23987 NNP denotes ]
T12175 23987-23988 . denotes .
T12176 23989-23991 PRP denotes We
T12177 23992-24003 VBD denotes transfected
T12178 24004-24009 JJ denotes human
T12179 24010-24017 JJ denotes primary
T12180 24018-24021 NNP denotes CD4
T12181 24021-24022 CD denotes +
T12182 24023-24024 NNP denotes T
T12183 24025-24030 NNS denotes cells
T12184 24030-24031 , denotes ,
T12185 24032-24034 IN denotes in
T12186 24035-24040 NN denotes vitro
T12187 24041-24055 VBD denotes differentiated
T12188 24056-24059 JJ denotes Th2
T12189 24060-24065 NNS denotes cells
T12190 24065-24066 , denotes ,
T12191 24067-24070 CC denotes and
T12192 24071-24077 NNP denotes Jurkat
T12193 24078-24083 NNS denotes cells
T12194 24084-24085 -LRB- denotes (
T12195 24085-24091 NNP denotes Jurkat
T12196 24092-24097 NNS denotes cells
T12197 24098-24101 VBP denotes are
T12198 24102-24107 VBN denotes known
T12199 24108-24110 TO denotes to
T12200 24111-24125 RB denotes constitutively
T12201 24126-24133 VB denotes express
T12202 24134-24139 NNP denotes GATA3
T12203 24140-24141 NNP denotes [
T12204 24141-24146 CD denotes 25,26
T12205 24146-24147 NNP denotes ]
T12206 24147-24148 -RRB- denotes )
T12207 24148-24149 , denotes ,
T12208 24150-24153 CC denotes and
T12209 24154-24156 PRP denotes we
T12210 24157-24165 VBD denotes measured
T12211 24166-24171 CD denotes FOXP3
T12212 24172-24180 NN denotes promoter
T12213 24181-24189 NN denotes activity
T12214 24189-24190 . denotes .
T12215 24191-24194 DT denotes The
T12216 24195-24203 NN denotes promoter
T12217 24204-24207 VBD denotes was
T12218 24208-24211 RB denotes not
T12219 24212-24218 JJ denotes active
T12220 24219-24221 IN denotes in
T12221 24222-24225 DT denotes the
T12222 24226-24242 JJ denotes GATA3-expressing
T12223 24243-24247 NN denotes cell
T12224 24248-24252 NN denotes line
T12225 24253-24259 NNP denotes Jurkat
T12226 24260-24262 CC denotes or
T12227 24263-24265 IN denotes in
T12228 24266-24269 DT denotes the
T12229 24270-24272 IN denotes in
T12230 24273-24293 JJ denotes vitro-differentiated
T12231 24294-24297 JJ denotes Th2
T12232 24298-24303 NNS denotes cells
T12233 24303-24304 , denotes ,
T12234 24305-24312 IN denotes whereas
T12235 24313-24316 DT denotes the
T12236 24317-24326 VBP denotes construct
T12237 24327-24330 VBD denotes was
T12238 24331-24337 JJ denotes active
T12239 24338-24340 IN denotes in
T12240 24341-24344 DT denotes the
T12241 24345-24348 NNP denotes CD4
T12242 24349-24354 NNS denotes cells
T12243 24354-24355 , denotes ,
T12244 24356-24361 WDT denotes which
T12245 24362-24369 VBP denotes express
T12246 24370-24371 DT denotes a
T12247 24372-24377 JJR denotes lower
T12248 24378-24384 NN denotes amount
T12249 24385-24387 IN denotes of
T12250 24388-24393 NNP denotes GATA3
T12251 24394-24395 -LRB- denotes (
T12252 24395-24401 NNP denotes Figure
T12253 24402-24404 NNP denotes 8A
T12254 24404-24405 -RRB- denotes )
T12255 24405-24406 . denotes .
T12256 24407-24421 NNP denotes Overexpression
T12257 24422-24424 IN denotes of
T12258 24425-24430 NNP denotes GATA3
T12259 24431-24433 IN denotes in
T12260 24434-24439 JJ denotes naive
T12261 24440-24441 NNP denotes T
T12262 24442-24447 NNS denotes cells
T12263 24448-24458 VBD denotes diminished
T12264 24459-24469 NN denotes luciferase
T12265 24470-24478 NN denotes activity
T12266 24479-24481 IN denotes of
T12267 24482-24485 DT denotes the
T12268 24486-24491 CD denotes FOXP3
T12269 24492-24500 NN denotes promoter
T12270 24501-24509 VBN denotes compared
T12271 24510-24514 IN denotes with
T12272 24515-24518 DT denotes the
T12273 24519-24526 NN denotes control
T12274 24527-24533 NN denotes vector
T12275 24534-24535 -LRB- denotes (
T12276 24535-24541 NN denotes Figure
T12277 24542-24544 NN denotes 8B
T12278 24544-24545 -RRB- denotes )
T12279 24545-24546 . denotes .
T12280 24547-24549 TO denotes To
T12281 24550-24557 RBR denotes further
T12282 24558-24565 VB denotes address
T12283 24566-24569 DT denotes the
T12284 24570-24578 NN denotes function
T12285 24579-24581 IN denotes of
T12286 24582-24585 DT denotes the
T12287 24586-24591 NNP denotes GATA3
T12288 24592-24596 NN denotes site
T12289 24596-24597 , denotes ,
T12290 24598-24600 PRP denotes we
T12291 24601-24609 VBD denotes inserted
T12292 24610-24611 DT denotes a
T12293 24612-24625 JJ denotes site-specific
T12294 24626-24634 NN denotes mutation
T12295 24635-24643 VBG denotes deleting
T12296 24644-24647 DT denotes the
T12297 24648-24661 JJ denotes GATA3-binding
T12298 24662-24666 NN denotes site
T12299 24666-24667 . denotes .
T12300 24668-24672 DT denotes This
T12301 24673-24681 NN denotes mutation
T12302 24682-24691 VBD denotes increased
T12303 24692-24702 NN denotes luciferase
T12304 24703-24711 NN denotes activity
T12305 24712-24714 IN denotes by
T12306 24715-24721 JJ denotes 3-fold
T12307 24722-24724 IN denotes in
T12308 24725-24731 NN denotes memory
T12309 24732-24735 NNP denotes CD4
T12310 24735-24736 NNP denotes +
T12311 24736-24742 NNP denotes CD45RO
T12312 24742-24743 VBD denotes +
T12313 24744-24745 NNP denotes T
T12314 24746-24751 NNS denotes cells
T12315 24751-24752 , denotes ,
T12316 24753-24760 IN denotes whereas
T12317 24761-24763 DT denotes no
T12318 24764-24774 NN denotes difference
T12319 24775-24778 VBD denotes was
T12320 24779-24787 VBN denotes observed
T12321 24788-24790 IN denotes in
T12322 24791-24796 JJ denotes naive
T12323 24797-24798 -LRB- denotes (
T12324 24798-24803 CD denotes GATA3
T12325 24804-24805 -RRB- denotes )
T12326 24806-24809 CD denotes CD4
T12327 24809-24810 CD denotes +
T12328 24810-24816 NNP denotes CD45RA
T12329 24816-24817 CD denotes +
T12330 24818-24819 NNP denotes T
T12331 24820-24825 NNS denotes cells
T12332 24825-24826 , denotes ,
T12333 24827-24836 VBG denotes revealing
T12334 24837-24838 DT denotes a
T12335 24839-24848 NN denotes repressor
T12336 24849-24857 NN denotes activity
T12337 24858-24860 IN denotes of
T12338 24861-24866 NNP denotes GATA3
T12339 24867-24869 IN denotes on
T12340 24870-24873 DT denotes the
T12341 24874-24879 CD denotes FOXP3
T12342 24880-24888 NN denotes promoter
T12343 24889-24890 -LRB- denotes (
T12344 24890-24896 NN denotes Figure
T12345 24897-24899 NN denotes 8C
T12346 24899-24900 -RRB- denotes )
T12347 24900-24901 . denotes .
T12348 24902-24913 RB denotes Furthermore
T12349 24914-24919 JJ denotes GATA3
T12350 24920-24925 NNS denotes binds
T12351 24926-24934 RB denotes directly
T12352 24935-24937 TO denotes to
T12353 24938-24941 DT denotes the
T12354 24942-24947 CD denotes FOXP3
T12355 24948-24956 NN denotes promoter
T12356 24957-24959 IN denotes as
T12357 24960-24972 VBN denotes investigated
T12358 24973-24975 IN denotes by
T12359 24976-24985 JJ denotes pull-down
T12360 24986-24991 NN denotes assay
T12361 24991-24992 . denotes .
T12362 24993-24996 NNP denotes HEK
T12363 24997-25002 NNS denotes cells
T12364 25003-25007 VBD denotes were
T12365 25008-25019 RB denotes transiently
T12366 25020-25031 VBN denotes transfected
T12367 25032-25036 IN denotes with
T12368 25037-25042 CD denotes GATA3
T12369 25043-25045 CC denotes or
T12370 25046-25047 DT denotes a
T12371 25048-25055 NN denotes control
T12372 25056-25062 NN denotes vector
T12373 25062-25063 , denotes ,
T12374 25064-25067 CC denotes and
T12375 25068-25078 VBG denotes increasing
T12376 25079-25086 NNS denotes amounts
T12377 25087-25089 IN denotes of
T12378 25090-25097 NNS denotes lysates
T12379 25098-25102 VBD denotes were
T12380 25103-25112 VBN denotes incubated
T12381 25113-25117 IN denotes with
T12382 25118-25134 NNS denotes oligonucleotides
T12383 25135-25145 VBG denotes containing
T12384 25146-25149 DT denotes the
T12385 25150-25155 NNP denotes GATA3
T12386 25156-25160 NN denotes site
T12387 25161-25163 IN denotes of
T12388 25164-25167 DT denotes the
T12389 25168-25173 CD denotes FOXP3
T12390 25174-25182 NN denotes promoter
T12391 25183-25185 CC denotes or
T12392 25186-25187 DT denotes a
T12393 25188-25195 NN denotes control
T12394 25196-25211 NN denotes oligonucleotide
T12395 25212-25216 IN denotes with
T12396 25217-25218 DT denotes a
T12397 25219-25226 VBN denotes mutated
T12398 25227-25240 JJ denotes GATA3-binding
T12399 25241-25245 NN denotes site
T12400 25245-25246 . denotes .
T12401 25247-25252 IN denotes After
T12402 25253-25256 DT denotes the
T12403 25257-25266 JJ denotes pull-down
T12404 25266-25267 , denotes ,
T12405 25268-25273 JJ denotes GATA3
T12406 25274-25281 JJ denotes binding
T12407 25282-25285 VBD denotes was
T12408 25286-25294 VBN denotes detected
T12409 25295-25297 IN denotes by
T12410 25298-25305 JJ denotes Western
T12411 25306-25310 NN denotes blot
T12412 25310-25311 . denotes .
T12413 25312-25321 RB denotes Similarly
T12414 25321-25322 , denotes ,
T12415 25323-25339 JJ denotes GATA3-expressing
T12416 25340-25343 JJ denotes Th2
T12417 25344-25349 NNS denotes cells
T12418 25350-25353 CC denotes and
T12419 25354-25359 NNP denotes iTreg
T12420 25360-25365 NNS denotes cells
T12421 25366-25370 VBD denotes were
T12422 25371-25380 VBN denotes subjected
T12423 25381-25383 TO denotes to
T12424 25384-25388 DT denotes this
T12425 25389-25397 NN denotes approach
T12426 25397-25398 . denotes .
T12427 25399-25403 RB denotes Only
T12428 25404-25407 NNP denotes HEK
T12429 25408-25413 NNS denotes cells
T12430 25414-25428 VBG denotes overexpressing
T12431 25429-25434 NNP denotes GATA3
T12432 25435-25438 CC denotes and
T12433 25439-25442 NNP denotes Th2
T12434 25443-25448 NNS denotes cells
T12435 25449-25455 VBD denotes showed
T12436 25456-25469 JJ denotes GATA3-binding
T12437 25470-25478 NN denotes activity
T12438 25479-25480 -LRB- denotes (
T12439 25480-25486 NN denotes Figure
T12440 25487-25489 CD denotes 8D
T12441 25490-25493 CC denotes and
T12442 25494-25496 CD denotes 8E
T12443 25496-25497 -RRB- denotes )
T12444 25497-25498 . denotes .
T12445 25499-25504 DT denotes These
T12446 25505-25516 NNS denotes experiments
T12447 25517-25529 VBD denotes demonstrated
T12448 25530-25534 IN denotes that
T12449 25535-25540 NNP denotes GATA3
T12450 25541-25546 VBZ denotes binds
T12451 25547-25550 DT denotes the
T12452 25551-25562 JJ denotes palindromic
T12453 25563-25568 CD denotes FOXP3
T12454 25569-25577 NN denotes promoter
T12455 25577-25578 . denotes .
T12456 25579-25581 TO denotes To
T12457 25582-25586 VB denotes gain
T12458 25587-25595 NNS denotes insights
T12459 25596-25600 IN denotes into
T12460 25601-25604 DT denotes the
T12461 25605-25607 IN denotes in
T12462 25608-25612 NN denotes vivo
T12463 25613-25622 NN denotes situation
T12464 25622-25623 , denotes ,
T12465 25624-25626 PRP denotes we
T12466 25627-25636 VBD denotes performed
T12467 25637-25638 DT denotes a
T12468 25639-25648 NN denotes chromatin
T12469 25649-25668 NN denotes immunoprecipitation
T12470 25669-25670 -LRB- denotes (
T12471 25670-25674 NNP denotes ChIP
T12472 25674-25675 -RRB- denotes )
T12473 25676-25681 VBG denotes using
T12474 25682-25684 DT denotes an
T12475 25685-25695 JJ denotes anti-GATA3
T12476 25696-25704 NN denotes antibody
T12477 25705-25708 CC denotes and
T12478 25709-25715 VBD denotes showed
T12479 25716-25720 IN denotes that
T12480 25721-25726 CD denotes GATA3
T12481 25727-25732 NNS denotes binds
T12482 25733-25735 TO denotes to
T12483 25736-25739 DT denotes the
T12484 25740-25745 CD denotes FOXP3
T12485 25746-25754 NN denotes promoter
T12486 25755-25761 NN denotes region
T12487 25762-25764 IN denotes in
T12488 25765-25768 JJ denotes Th2
T12489 25769-25774 NNS denotes cells
T12490 25774-25775 , denotes ,
T12491 25776-25779 CC denotes but
T12492 25780-25783 RB denotes not
T12493 25784-25786 IN denotes in
T12494 25787-25792 NNP denotes iTreg
T12495 25793-25798 NNS denotes cells
T12496 25799-25800 -LRB- denotes (
T12497 25800-25806 NN denotes Figure
T12498 25807-25809 CD denotes 7F
T12499 25809-25810 -RRB- denotes )
T12500 25810-25811 . denotes .
T12501 25812-25817 VBN denotes Taken
T12502 25818-25826 RB denotes together
T12503 25827-25832 DT denotes these
T12504 25833-25837 NNS denotes data
T12505 25838-25849 VBP denotes demonstrate
T12506 25850-25854 IN denotes that
T12507 25855-25858 DT denotes the
T12508 25859-25872 NN denotes GATA3-binding
T12509 25873-25875 TO denotes to
T12510 25876-25879 DT denotes the
T12511 25880-25885 CD denotes FOXP3
T12512 25886-25894 NN denotes promoter
T12513 25895-25897 VBZ denotes is
T12514 25898-25908 VBG denotes repressing
T12515 25909-25914 CD denotes FOXP3
T12516 25915-25925 NN denotes expression
T12517 25925-25926 . denotes .
T15144 27625-27628 DT denotes The
T15145 27629-27636 JJ denotes current
T15146 27637-27642 NN denotes study
T15147 27643-27650 VBZ denotes reveals
T15148 27651-27655 IN denotes that
T15149 27656-27661 CD denotes FOXP3
T15150 27662-27671 NN denotes induction
T15151 27671-27672 , denotes ,
T15152 27673-27675 DT denotes an
T15153 27676-27685 JJ denotes important
T15154 27686-27690 NN denotes step
T15155 27691-27693 IN denotes in
T15156 27694-27699 NNP denotes iTreg
T15157 27700-27710 NN denotes commitment
T15158 27710-27711 , denotes ,
T15159 27712-27714 VBZ denotes is
T15160 27715-27724 VBN denotes inhibited
T15161 27725-27727 IN denotes by
T15162 27728-27733 NNP denotes GATA3
T15163 27733-27734 , denotes ,
T15164 27735-27740 WDT denotes which
T15165 27741-27743 VBZ denotes is
T15166 27744-27747 DT denotes the
T15167 27748-27751 JJ denotes key
T15168 27752-27761 NN denotes regulator
T15169 27762-27765 IN denotes for
T15170 27766-27778 NN denotes polarization
T15171 27779-27785 IN denotes toward
T15172 27786-27789 JJ denotes Th2
T15173 27790-27795 NNS denotes cells
T15174 27795-27796 . denotes .
T15175 27797-27802 IN denotes After
T15176 27803-27818 NN denotes differentiation
T15177 27818-27819 , denotes ,
T15178 27820-27823 DT denotes the
T15179 27824-27832 NN denotes effector
T15180 27833-27836 JJ denotes Th2
T15181 27837-27842 NNS denotes cells
T15182 27843-27849 VBP denotes become
T15183 27850-27860 JJ denotes refractory
T15184 27861-27863 TO denotes to
T15185 27864-27874 NN denotes conversion
T15186 27875-27879 IN denotes into
T15187 27880-27881 DT denotes a
T15188 27882-27887 CD denotes FOXP3
T15189 27887-27888 NN denotes +
T15190 27889-27898 NN denotes phenotype
T15191 27898-27899 . denotes .
T15192 27900-27902 IN denotes In
T15193 27903-27913 NN denotes accordance
T15194 27914-27918 IN denotes with
T15195 27919-27924 JJ denotes other
T15196 27925-27932 NNS denotes studies
T15197 27932-27933 , denotes ,
T15198 27934-27936 PRP denotes we
T15199 27937-27942 VBD denotes found
T15200 27943-27947 IN denotes that
T15201 27948-27951 CD denotes CD4
T15202 27951-27952 CD denotes +
T15203 27952-27956 NNS denotes CD25
T15204 27958-27963 NNS denotes cells
T15205 27964-27968 VBD denotes were
T15206 27969-27973 JJ denotes able
T15207 27974-27976 TO denotes to
T15208 27977-27988 VB denotes up-regulate
T15209 27989-27994 CD denotes FOXP3
T15210 27995-27996 NNP denotes [
T15211 27996-28001 CD denotes 12,27
T15212 28001-28002 NNP denotes ]
T15213 28002-28003 . denotes .
T15214 28004-28021 JJ denotes Already-committed
T15215 28022-28027 NNS denotes cells
T15216 28028-28032 JJ denotes such
T15217 28033-28035 IN denotes as
T15218 28036-28042 NN denotes memory
T15219 28043-28044 NNP denotes T
T15220 28045-28050 NNS denotes cells
T15221 28051-28054 CC denotes and
T15222 28055-28058 JJ denotes Th1
T15223 28059-28064 NNS denotes cells
T15224 28065-28071 VBD denotes showed
T15225 28072-28076 RB denotes only
T15226 28077-28085 JJ denotes moderate
T15227 28086-28089 CC denotes and
T15228 28090-28099 JJ denotes transient
T15229 28100-28105 CD denotes FOXP3
T15230 28106-28115 NN denotes induction
T15231 28115-28116 , denotes ,
T15232 28117-28122 WDT denotes which
T15233 28123-28125 VBZ denotes is
T15234 28126-28129 RB denotes not
T15235 28130-28140 JJ denotes sufficient
T15236 28141-28143 TO denotes to
T15237 28144-28150 VB denotes change
T15238 28151-28154 DT denotes the
T15239 28155-28164 NN denotes phenotype
T15240 28165-28171 IN denotes toward
T15241 28172-28173 DT denotes a
T15242 28174-28184 JJ denotes regulatory
T15243 28185-28186 NNP denotes T
T15244 28187-28191 NN denotes cell
T15245 28192-28199 NN denotes profile
T15246 28199-28200 . denotes .
T15247 28201-28203 IN denotes In
T15248 28204-28212 NN denotes contrast
T15249 28212-28213 , denotes ,
T15250 28214-28219 JJ denotes naive
T15251 28220-28221 NNP denotes T
T15252 28222-28227 NNS denotes cells
T15253 28228-28233 MD denotes could
T15254 28234-28245 RB denotes efficiently
T15255 28246-28257 VB denotes up-regulate
T15256 28258-28263 CD denotes FOXP3
T15257 28264-28268 WRB denotes when
T15258 28269-28276 VBN denotes treated
T15259 28277-28281 IN denotes with
T15260 28282-28287 JJ denotes TGF-β
T15261 28288-28290 TO denotes to
T15262 28291-28297 VB denotes induce
T15263 28298-28303 NNP denotes iTreg
T15264 28304-28309 NNS denotes cells
T15265 28310-28311 NNP denotes [
T15266 28311-28316 CD denotes 10,28
T15267 28317-28319 CD denotes 34
T15268 28319-28320 NNP denotes ]
T15269 28320-28321 , denotes ,
T15270 28322-28332 VBG denotes suggesting
T15271 28333-28337 IN denotes that
T15272 28338-28343 CD denotes FOXP3
T15273 28344-28349 VBZ denotes plays
T15274 28350-28352 DT denotes an
T15275 28353-28362 JJ denotes important
T15276 28363-28367 NN denotes role
T15277 28368-28370 IN denotes in
T15278 28371-28374 DT denotes the
T15279 28375-28380 JJ denotes early
T15280 28381-28396 NN denotes differentiation
T15281 28397-28404 NN denotes process
T15282 28405-28408 CC denotes and
T15283 28409-28412 MD denotes may
T15284 28413-28416 VB denotes act
T15285 28417-28419 IN denotes in
T15286 28420-28421 DT denotes a
T15287 28422-28425 NN denotes way
T15288 28426-28433 JJ denotes similar
T15289 28434-28436 TO denotes to
T15290 28437-28441 DT denotes that
T15291 28442-28447 VBN denotes known
T15292 28448-28451 IN denotes for
T15293 28452-28455 DT denotes the
T15294 28456-28463 NNP denotes Th1/Th2
T15295 28464-28472 NN denotes decision
T15296 28473-28480 NNS denotes factors
T15297 28481-28486 NNP denotes T-bet
T15298 28487-28490 CC denotes and
T15299 28491-28496 NNP denotes GATA3
T15300 28496-28497 . denotes .
T15301 28498-28502 DT denotes This
T15302 28503-28513 NN denotes commitment
T15303 28514-28516 VBZ denotes is
T15304 28517-28530 VBN denotes characterized
T15305 28531-28533 IN denotes by
T15306 28534-28545 JJ denotes competitive
T15307 28546-28556 NN denotes expression
T15308 28557-28559 IN denotes of
T15309 28560-28565 DT denotes these
T15310 28566-28573 NNS denotes factors
T15311 28574-28575 NNP denotes [
T15312 28575-28580 CD denotes 35,36
T15313 28580-28581 NNP denotes ]
T15314 28581-28582 , denotes ,
T15315 28583-28588 WDT denotes which
T15316 28589-28591 PRP denotes we
T15317 28592-28596 RB denotes also
T15318 28597-28605 VBD denotes observed
T15319 28606-28608 IN denotes in
T15320 28609-28624 VBG denotes differentiating
T15321 28625-28630 CD denotes FOXP3
T15322 28630-28631 CD denotes +
T15323 28632-28637 NNP denotes iTreg
T15324 28638-28643 NNS denotes cells
T15325 28644-28653 VBG denotes including
T15326 28654-28655 DT denotes a
T15327 28656-28661 NN denotes phase
T15328 28662-28664 IN denotes of
T15329 28665-28678 NN denotes co-expression
T15330 28678-28679 , denotes ,
T15331 28680-28685 WDT denotes which
T15332 28686-28691 VBZ denotes turns
T15333 28692-28696 IN denotes into
T15334 28697-28711 VBG denotes nonoverlapping
T15335 28712-28722 NN denotes expression
T15336 28723-28727 IN denotes upon
T15337 28728-28737 VBN denotes completed
T15338 28738-28753 NN denotes differentiation
T15339 28753-28754 . denotes .
T15340 28755-28757 IN denotes In
T15341 28758-28762 DT denotes this
T15342 28763-28774 JJ denotes competitive
T15343 28775-28782 NN denotes process
T15344 28783-28788 NN denotes TGF-β
T15345 28789-28797 VBD denotes appeared
T15346 28798-28800 TO denotes to
T15347 28801-28803 VB denotes be
T15348 28804-28813 JJ denotes mandatory
T15349 28814-28817 IN denotes for
T15350 28818-28821 DT denotes the
T15351 28822-28831 NN denotes induction
T15352 28832-28834 IN denotes of
T15353 28835-28840 CD denotes FOXP3
T15354 28840-28841 , denotes ,
T15355 28842-28850 RB denotes possibly
T15356 28851-28853 IN denotes by
T15357 28854-28861 VBG denotes keeping
T15358 28862-28865 DT denotes the
T15359 28866-28876 NN denotes expression
T15360 28877-28879 IN denotes of
T15361 28880-28885 NNP denotes GATA3
T15362 28886-28889 CC denotes and
T15363 28890-28895 NNP denotes T-bet
T15364 28896-28899 JJ denotes low
T15365 28900-28901 NNP denotes [
T15366 28901-28906 CD denotes 37,38
T15367 28906-28907 NNP denotes ]
T15368 28907-28908 . denotes .
T15369 28909-28911 IN denotes In
T15370 28912-28920 NN denotes contrast
T15371 28920-28921 , denotes ,
T15372 28922-28937 VBG denotes differentiating
T15373 28938-28943 JJ denotes naive
T15374 28944-28945 NNP denotes T
T15375 28946-28951 NNS denotes cells
T15376 28952-28954 IN denotes in
T15377 28955-28958 DT denotes the
T15378 28959-28966 NN denotes absence
T15379 28967-28969 IN denotes of
T15380 28970-28982 NN denotes polarization
T15381 28983-28990 NNS denotes factors
T15382 28991-28992 -LRB- denotes (
T15383 28992-28995 CD denotes Th0
T15384 28995-28996 -RRB- denotes )
T15385 28997-29001 JJ denotes such
T15386 29002-29004 IN denotes as
T15387 29005-29009 NN denotes IL-4
T15388 29009-29010 , denotes ,
T15389 29011-29016 NN denotes IL-12
T15390 29016-29017 , denotes ,
T15391 29018-29020 CC denotes or
T15392 29021-29026 JJ denotes TGF-β
T15393 29027-29033 VBD denotes showed
T15394 29034-29038 RB denotes only
T15395 29039-29040 DT denotes a
T15396 29041-29050 NN denotes transient
T15397 29051-29056 CD denotes FOXP3
T15398 29057-29067 NN denotes expression
T15399 29068-29071 CC denotes and
T15400 29072-29078 VBD denotes failed
T15401 29079-29081 TO denotes to
T15402 29082-29090 VB denotes generate
T15403 29091-29092 DT denotes a
T15404 29093-29103 NN denotes population
T15405 29104-29106 IN denotes of
T15406 29107-29123 JJ denotes FOXP3-expressing
T15407 29124-29129 NNS denotes cells
T15408 29129-29130 , denotes ,
T15409 29131-29134 CC denotes but
T15410 29135-29140 NNP denotes GATA3
T15411 29141-29144 CC denotes and
T15412 29145-29150 NNP denotes T-bet
T15413 29151-29155 VBD denotes were
T15414 29156-29168 JJ denotes up-regulated
T15415 29169-29170 -LRB- denotes (
T15416 29170-29181 JJ denotes unpublished
T15417 29182-29186 NNS denotes data
T15418 29186-29187 -RRB- denotes )
T15419 29187-29188 . denotes .
T15420 29189-29202 RB denotes Interestingly
T15421 29202-29203 , denotes ,
T15422 29204-29206 IN denotes as
T15423 29207-29209 PRP denotes we
T15424 29210-29213 CC denotes and
T15425 29214-29220 NNS denotes others
T15426 29221-29231 RB denotes previously
T15427 29232-29241 VBN denotes described
T15428 29241-29242 , denotes ,
T15429 29243-29258 JJ denotes FOXP3-promoting
T15430 29259-29266 NNS denotes factors
T15431 29266-29267 , denotes ,
T15432 29268-29272 JJ denotes such
T15433 29273-29275 IN denotes as
T15434 29276-29289 VBN denotes dexamethasone
T15435 29290-29291 NNP denotes [
T15436 29291-29293 CD denotes 39
T15437 29293-29294 NNP denotes ]
T15438 29294-29295 , denotes ,
T15439 29296-29302 NNP denotes CTLA-4
T15440 29303-29304 NNP denotes [
T15441 29304-29306 CD denotes 40
T15442 29306-29307 NNP denotes ]
T15443 29307-29308 , denotes ,
T15444 29309-29312 CC denotes and
T15445 29313-29322 VBZ denotes estrogens
T15446 29323-29324 NNP denotes [
T15447 29324-29326 CD denotes 41
T15448 29326-29327 NNP denotes ]
T15449 29327-29328 , denotes ,
T15450 29329-29332 VBP denotes are
T15451 29333-29337 RB denotes also
T15452 29338-29343 VBN denotes known
T15453 29344-29346 IN denotes as
T15454 29347-29357 NNS denotes inhibitors
T15455 29358-29360 IN denotes of
T15456 29361-29366 NNP denotes GATA3
T15457 29367-29377 NN denotes expression
T15458 29378-29379 NNP denotes [
T15459 29379-29381 CD denotes 42
T15460 29382-29384 CD denotes 45
T15461 29384-29385 NN denotes ]
T15462 29385-29386 . denotes .
T15463 29387-29396 RB denotes Therefore
T15464 29397-29402 NNP denotes GATA3
T15465 29403-29406 RB denotes not
T15466 29407-29411 RB denotes only
T15467 29412-29419 VBZ denotes induces
T15468 29420-29435 NN denotes differentiation
T15469 29436-29440 IN denotes into
T15470 29441-29444 JJ denotes Th2
T15471 29445-29450 NNS denotes cells
T15472 29451-29454 CC denotes but
T15473 29455-29459 RB denotes also
T15474 29460-29468 VBZ denotes inhibits
T15475 29469-29474 CD denotes FOXP3
T15476 29475-29485 NN denotes expression
T15477 29486-29489 CC denotes and
T15478 29490-29500 NN denotes commitment
T15479 29501-29505 IN denotes into
T15480 29506-29511 NNP denotes iTreg
T15481 29512-29517 NNS denotes cells
T15482 29517-29518 . denotes .
T15483 29519-29522 DT denotes The
T15484 29523-29526 JJ denotes Th2
T15485 29527-29535 NN denotes cytokine
T15486 29536-29540 NN denotes IL-4
T15487 29541-29544 CC denotes but
T15488 29545-29548 RB denotes not
T15489 29549-29554 JJ denotes IL-13
T15490 29555-29556 -LRB- denotes (
T15491 29556-29567 JJ denotes unpublished
T15492 29568-29572 NNS denotes data
T15493 29572-29573 -RRB- denotes )
T15494 29574-29577 VBD denotes was
T15495 29578-29582 JJ denotes able
T15496 29583-29585 TO denotes to
T15497 29586-29593 VB denotes inhibit
T15498 29594-29599 NNP denotes TGF-β
T15499 29600-29608 VBD denotes mediated
T15500 29609-29614 CD denotes FOXP3
T15501 29615-29624 NN denotes induction
T15502 29625-29628 CC denotes and
T15503 29629-29638 RB denotes therefore
T15504 29639-29648 VBD denotes prevented
T15505 29649-29659 NN denotes conversion
T15506 29660-29664 IN denotes into
T15507 29665-29668 DT denotes the
T15508 29669-29679 JJ denotes regulatory
T15509 29680-29689 NN denotes phenotype
T15510 29689-29690 . denotes .
T15511 29691-29693 TO denotes To
T15512 29694-29699 VB denotes prove
T15513 29700-29703 DT denotes the
T15514 29704-29714 JJ denotes inhibitory
T15515 29715-29721 NN denotes effect
T15516 29722-29724 IN denotes of
T15517 29725-29729 NN denotes IL-4
T15518 29730-29732 IN denotes on
T15519 29733-29742 JJ denotes inducible
T15520 29743-29745 CC denotes or
T15521 29746-29753 JJ denotes natural
T15522 29754-29758 NNP denotes Treg
T15523 29759-29769 NN denotes commitment
T15524 29770-29772 IN denotes in
T15525 29773-29777 NN denotes vivo
T15526 29777-29778 , denotes ,
T15527 29779-29781 PRP denotes we
T15528 29782-29789 VBD denotes treated
T15529 29790-29794 NNS denotes mice
T15530 29795-29799 IN denotes with
T15531 29800-29804 NN denotes IL-4
T15532 29805-29808 CC denotes and
T15533 29809-29818 NN denotes anti-IL-4
T15534 29818-29819 . denotes .
T15535 29820-29824 DT denotes This
T15536 29825-29828 VBZ denotes has
T15537 29829-29833 VBN denotes been
T15538 29834-29839 VBN denotes shown
T15539 29840-29842 TO denotes to
T15540 29843-29851 VB denotes increase
T15541 29852-29855 DT denotes the
T15542 29856-29862 NN denotes effect
T15543 29863-29865 IN denotes of
T15544 29866-29869 DT denotes the
T15545 29870-29878 NN denotes cytokine
T15546 29879-29881 IN denotes in
T15547 29882-29886 NN denotes vivo
T15548 29887-29888 NNP denotes [
T15549 29888-29890 CD denotes 19
T15550 29890-29891 NNP denotes ]
T15551 29891-29892 . denotes .
T15552 29893-29897 RB denotes Only
T15553 29898-29901 DT denotes the
T15554 29902-29906 NN denotes IL-4
T15555 29906-29907 NN denotes /
T15556 29907-29911 NN denotes IL-4
T15557 29912-29915 NNP denotes mAb
T15558 29916-29923 JJ denotes complex
T15559 29924-29932 VBD denotes resulted
T15560 29933-29935 IN denotes in
T15561 29936-29937 DT denotes a
T15562 29938-29946 NN denotes decrease
T15563 29947-29949 IN denotes of
T15564 29950-29953 DT denotes the
T15565 29954-29960 NN denotes amount
T15566 29961-29963 IN denotes of
T15567 29964-29971 JJ denotes natural
T15568 29972-29974 CC denotes or
T15569 29975-29984 JJ denotes inducible
T15570 29985-29989 NNP denotes Treg
T15571 29990-29991 -LRB- denotes (
T15572 29991-29995 NNP denotes CD25
T15573 29995-29996 NNP denotes +
T15574 29997-30000 CC denotes and
T15575 30001-30006 NNP denotes Foxp3
T15576 30006-30007 NNP denotes +
T15577 30007-30008 -RRB- denotes )
T15578 30009-30014 NNS denotes cells
T15579 30015-30016 CD denotes 7
T15580 30017-30018 LS denotes d
T15581 30019-30024 IN denotes after
T15582 30025-30034 NN denotes treatment
T15583 30034-30035 . denotes .
T15584 30036-30039 PRP$ denotes Our
T15585 30040-30047 NNS denotes results
T15586 30048-30055 VBP denotes suggest
T15587 30056-30060 IN denotes that
T15588 30061-30065 NN denotes IL-4
T15589 30066-30068 VBZ denotes is
T15590 30069-30073 RB denotes only
T15591 30074-30085 VBG denotes interfering
T15592 30086-30090 IN denotes with
T15593 30091-30094 DT denotes the
T15594 30095-30110 NN denotes differentiation
T15595 30111-30113 IN denotes of
T15596 30114-30119 JJ denotes naive
T15597 30120-30121 NNP denotes T
T15598 30122-30127 NNS denotes cells
T15599 30128-30132 IN denotes into
T15600 30133-30138 NNP denotes iTreg
T15601 30139-30144 NNS denotes cells
T15602 30144-30145 . denotes .
T15603 30146-30149 CC denotes But
T15604 30150-30155 IN denotes since
T15605 30156-30157 DT denotes a
T15606 30158-30169 NN denotes distinction
T15607 30170-30172 IN denotes of
T15608 30173-30178 NNP denotes nTreg
T15609 30179-30182 CC denotes and
T15610 30183-30188 NNP denotes iTreg
T15611 30189-30194 NNS denotes cells
T15612 30195-30197 VBZ denotes is
T15613 30198-30207 RB denotes currently
T15614 30208-30211 RB denotes not
T15615 30212-30220 JJ denotes possible
T15616 30220-30221 , denotes ,
T15617 30222-30229 IN denotes because
T15618 30230-30235 NNP denotes iTreg
T15619 30236-30241 NNS denotes cells
T15620 30242-30246 RB denotes also
T15621 30247-30258 RB denotes transiently
T15622 30259-30266 JJ denotes express
T15623 30267-30271 NNS denotes CD25
T15624 30272-30277 IN denotes after
T15625 30278-30288 NN denotes activation
T15626 30288-30289 , denotes ,
T15627 30290-30292 PRP denotes we
T15628 30293-30296 MD denotes can
T15629 30296-30299 RB denotes not
T15630 30300-30307 VB denotes exclude
T15631 30308-30312 DT denotes that
T15632 30313-30317 NN denotes IL-4
T15633 30318-30321 MD denotes may
T15634 30322-30326 RB denotes also
T15635 30327-30334 VB denotes inhibit
T15636 30335-30340 JJ denotes Foxp3
T15637 30341-30351 NN denotes expression
T15638 30352-30354 IN denotes in
T15639 30355-30360 NNP denotes nTreg
T15640 30361-30366 NNS denotes cells
T15641 30367-30369 IN denotes in
T15642 30370-30374 NN denotes vivo
T15643 30375-30377 CC denotes or
T15644 30378-30382 IN denotes that
T15645 30383-30393 JJ denotes additional
T15646 30394-30401 NNS denotes effects
T15647 30402-30405 MD denotes may
T15648 30406-30416 VB denotes contribute
T15649 30417-30419 TO denotes to
T15650 30420-30423 DT denotes the
T15651 30424-30432 JJ denotes observed
T15652 30433-30437 NN denotes drop
T15653 30438-30440 IN denotes in
T15654 30441-30446 JJ denotes Foxp3
T15655 30447-30457 NN denotes expression
T15656 30457-30458 . denotes .
T15657 30459-30463 NN denotes IL-4
T15658 30464-30467 VBZ denotes has
T15659 30468-30475 RB denotes already
T15660 30476-30480 VBN denotes been
T15661 30481-30486 VBN denotes shown
T15662 30487-30489 TO denotes to
T15663 30490-30500 RB denotes negatively
T15664 30501-30509 VB denotes regulate
T15665 30510-30513 DT denotes the
T15666 30514-30525 NN denotes development
T15667 30526-30528 IN denotes of
T15668 30529-30534 JJ denotes naive
T15669 30535-30536 NNP denotes T
T15670 30537-30542 NNS denotes cells
T15671 30543-30547 IN denotes into
T15672 30548-30551 CD denotes Th1
T15673 30552-30554 CC denotes or
T15674 30555-30558 DT denotes the
T15675 30559-30564 NNP denotes IL-17
T15676 30565-30574 VBG denotes producing
T15677 30575-30576 NNP denotes T
T15678 30577-30582 NNS denotes cells
T15679 30583-30584 -LRB- denotes (
T15680 30584-30588 NNP denotes Th17
T15681 30588-30589 -RRB- denotes )
T15682 30590-30591 NNP denotes [
T15683 30591-30596 CD denotes 46,47
T15684 30596-30597 NNP denotes ]
T15685 30597-30598 . denotes .
T15686 30599-30606 JJ denotes Similar
T15687 30607-30614 NNS denotes effects
T15688 30615-30619 VBP denotes have
T15689 30620-30624 VBN denotes been
T15690 30625-30633 RB denotes recently
T15691 30634-30643 VBN denotes described
T15692 30644-30647 IN denotes for
T15693 30648-30652 NNP denotes IL-6
T15694 30652-30653 , denotes ,
T15695 30654-30659 WDT denotes which
T15696 30659-30660 , denotes ,
T15697 30661-30669 VBN denotes combined
T15698 30670-30674 IN denotes with
T15699 30675-30680 NNP denotes TGF-β
T15700 30680-30681 , denotes ,
T15701 30682-30690 VBZ denotes inhibits
T15702 30691-30694 DT denotes the
T15703 30695-30705 NN denotes generation
T15704 30706-30708 IN denotes of
T15705 30709-30714 NNP denotes iTreg
T15706 30715-30720 NNS denotes cells
T15707 30721-30724 CC denotes and
T15708 30725-30732 VBZ denotes induces
T15709 30733-30748 NN denotes differentiation
T15710 30749-30753 IN denotes into
T15711 30754-30757 DT denotes the
T15712 30758-30762 NNP denotes Th17
T15713 30763-30768 NNS denotes cells
T15714 30769-30771 IN denotes by
T15715 30772-30774 DT denotes an
T15716 30775-30782 JJ denotes unknown
T15717 30783-30792 NN denotes mechanism
T15718 30793-30794 NNP denotes [
T15719 30794-30799 CD denotes 48,49
T15720 30799-30800 NNP denotes ]
T15721 30800-30801 . denotes .
T15722 30802-30806 RB denotes Thus
T15723 30807-30810 DT denotes the
T15724 30811-30823 NN denotes polarization
T15725 30824-30828 IN denotes into
T15726 30829-30834 NNP denotes iTreg
T15727 30835-30840 NNS denotes cells
T15728 30841-30843 VBZ denotes is
T15729 30844-30854 RB denotes negatively
T15730 30855-30864 VBN denotes regulated
T15731 30865-30867 IN denotes by
T15732 30868-30871 DT denotes the
T15733 30872-30880 NN denotes effector
T15734 30881-30890 VBZ denotes cytokines
T15735 30891-30895 NN denotes IL-4
T15736 30896-30899 CC denotes and
T15737 30900-30904 NN denotes IL-6
T15738 30904-30905 . denotes .
T15739 30906-30910 NN denotes IL-4
T15740 30911-30914 VBZ denotes has
T15741 30915-30919 VBN denotes been
T15742 30920-30930 RB denotes previously
T15743 30931-30936 VBN denotes shown
T15744 30937-30939 TO denotes to
T15745 30940-30946 VB denotes induce
T15746 30947-30950 DT denotes the
T15747 30951-30961 NN denotes generation
T15748 30962-30964 IN denotes of
T15749 30965-30970 CD denotes FOXP3
T15750 30970-30971 CD denotes +
T15751 30972-30976 NNP denotes Treg
T15752 30977-30982 NNS denotes cells
T15753 30983-30986 IN denotes out
T15754 30987-30989 IN denotes of
T15755 30990-30993 NNP denotes CD4
T15756 30993-30994 NNP denotes +
T15757 30994-30998 NNP denotes CD25
T15758 31000-31001 NNP denotes [
T15759 31001-31003 CD denotes 50
T15760 31003-31004 NNP denotes ]
T15761 31004-31005 . denotes .
T15762 31006-31008 IN denotes In
T15763 31009-31014 DT denotes those
T15764 31015-31026 NNS denotes experiments
T15765 31026-31027 , denotes ,
T15766 31028-31031 DT denotes the
T15767 31032-31046 NNS denotes concentrations
T15768 31047-31049 IN denotes of
T15769 31050-31054 NN denotes IL-4
T15770 31055-31059 VBN denotes used
T15771 31060-31064 VBD denotes were
T15772 31065-31068 JJ denotes low
T15773 31068-31069 , denotes ,
T15774 31070-31073 CC denotes and
T15775 31074-31076 IN denotes as
T15776 31077-31079 PRP denotes we
T15777 31080-31084 RB denotes also
T15778 31085-31093 VBD denotes observed
T15779 31093-31094 , denotes ,
T15780 31095-31099 NN denotes IL-4
T15781 31100-31102 IN denotes at
T15782 31103-31106 JJ denotes low
T15783 31107-31120 NN denotes concentration
T15784 31121-31129 RB denotes slightly
T15785 31130-31138 JJ denotes enhanced
T15786 31139-31144 CD denotes FOXP3
T15787 31145-31155 NN denotes expression
T15788 31155-31156 . denotes .
T15789 31157-31168 RB denotes Importantly
T15790 31168-31169 , denotes ,
T15791 31170-31175 DT denotes these
T15792 31176-31190 NNS denotes concentrations
T15793 31191-31195 VBD denotes were
T15794 31196-31199 RB denotes not
T15795 31200-31210 JJ denotes sufficient
T15796 31211-31213 TO denotes to
T15797 31214-31220 VB denotes induce
T15798 31221-31226 NNP denotes GATA3
T15799 31227-31237 NN denotes expression
T15800 31237-31238 . denotes .
T15801 31239-31243 NN denotes IL-4
T15802 31244-31247 MD denotes may
T15803 31248-31253 VB denotes favor
T15804 31254-31267 NN denotes proliferation
T15805 31268-31270 IN denotes of
T15806 31271-31276 NNP denotes nTreg
T15807 31277-31282 NNS denotes cells
T15808 31283-31284 NNP denotes [
T15809 31284-31286 CD denotes 47
T15810 31286-31287 NNP denotes ]
T15811 31288-31290 CC denotes or
T15812 31291-31299 RB denotes directly
T15813 31300-31308 VB denotes regulate
T15814 31309-31314 CD denotes FOXP3
T15815 31315-31325 NN denotes expression
T15816 31326-31328 IN denotes in
T15817 31329-31330 DT denotes a
T15818 31331-31345 JJ denotes STAT-dependent
T15819 31346-31353 NN denotes fashion
T15820 31354-31355 NNP denotes [
T15821 31355-31357 CD denotes 51
T15822 31357-31358 NNP denotes ]
T15823 31358-31359 . denotes .
T15824 31360-31365 IN denotes Since
T15825 31366-31371 NN denotes IL-13
T15826 31372-31376 VBZ denotes does
T15827 31377-31380 RB denotes not
T15828 31381-31392 RB denotes effectively
T15829 31393-31399 VB denotes reduce
T15830 31400-31405 CD denotes FOXP3
T15831 31406-31409 CC denotes and
T15832 31410-31415 VBZ denotes fails
T15833 31416-31418 TO denotes to
T15834 31419-31425 VB denotes induce
T15835 31426-31431 NNP denotes GATA3
T15836 31431-31432 , denotes ,
T15837 31433-31435 PRP denotes we
T15838 31436-31448 VBD denotes hypothesized
T15839 31449-31453 IN denotes that
T15840 31454-31457 DT denotes the
T15841 31458-31462 NN denotes IL-4
T15842 31463-31472 JJ denotes dependent
T15843 31473-31483 NN denotes inhibition
T15844 31484-31486 IN denotes of
T15845 31487-31492 CD denotes FOXP3
T15846 31493-31498 MD denotes could
T15847 31499-31501 VB denotes be
T15848 31502-31510 VBN denotes mediated
T15849 31511-31513 IN denotes by
T15850 31514-31519 NNP denotes GATA3
T15851 31519-31520 . denotes .
T15852 31521-31523 IN denotes In
T15853 31524-31528 NN denotes fact
T15854 31528-31529 , denotes ,
T15855 31530-31544 VBG denotes GATA3-inducing
T15856 31545-31549 NN denotes IL-4
T15857 31550-31564 NNS denotes concentrations
T15858 31565-31574 VBD denotes repressed
T15859 31575-31580 NNP denotes TGF-β
T15860 31581-31589 VBD denotes mediated
T15861 31590-31595 CD denotes FOXP3
T15862 31596-31606 NN denotes expression
T15863 31606-31607 , denotes ,
T15864 31608-31615 IN denotes whereas
T15865 31616-31620 NN denotes IL-4
T15866 31621-31623 RB denotes as
T15867 31624-31628 RB denotes well
T15868 31629-31631 IN denotes as
T15869 31632-31637 JJ denotes TGF-β
T15870 31638-31647 VBG denotes signaling
T15871 31648-31652 VBD denotes were
T15872 31653-31659 JJ denotes intact
T15873 31659-31660 . denotes .
T15874 31661-31665 DT denotes This
T15875 31666-31672 NN denotes result
T15876 31673-31682 VBD denotes suggested
T15877 31683-31684 DT denotes a
T15878 31685-31696 JJ denotes competitive
T15879 31697-31706 NN denotes mechanism
T15880 31707-31714 IN denotes between
T15881 31715-31720 NNP denotes GATA3
T15882 31721-31724 CC denotes and
T15883 31725-31730 NNP denotes FOXP3
T15884 31731-31744 NN denotes transcription
T15885 31745-31752 NNS denotes factors
T15886 31753-31755 IN denotes in
T15887 31756-31767 VBG denotes determining
T15888 31768-31775 NN denotes lineage
T15889 31776-31786 NN denotes commitment
T15890 31787-31793 IN denotes during
T15891 31794-31797 DT denotes the
T15892 31798-31803 JJ denotes early
T15893 31804-31809 NN denotes phase
T15894 31810-31812 IN denotes of
T15895 31813-31828 NN denotes differentiation
T15896 31828-31829 . denotes .
T15897 31830-31841 RB denotes Accordingly
T15898 31841-31842 , denotes ,
T15899 31843-31845 PRP denotes we
T15900 31846-31858 VBD denotes investigated
T15901 31859-31868 RB denotes naturally
T15902 31869-31873 JJ denotes high
T15903 31874-31890 JJ denotes GATA3-expressing
T15904 31891-31896 NNS denotes cells
T15905 31897-31900 CC denotes and
T15906 31901-31910 VBD denotes confirmed
T15907 31911-31914 DT denotes the
T15908 31915-31922 NN denotes absence
T15909 31923-31925 IN denotes of
T15910 31926-31931 CD denotes FOXP3
T15911 31931-31932 . denotes .
T15912 31933-31940 NN denotes Protein
T15913 31941-31953 NN denotes transduction
T15914 31954-31956 IN denotes of
T15915 31957-31962 CD denotes GATA3
T15916 31963-31967 IN denotes into
T15917 31968-31973 JJ denotes naive
T15918 31974-31975 NNP denotes T
T15919 31976-31981 NNS denotes cells
T15920 31982-31991 VBD denotes inhibited
T15921 31992-31997 CD denotes FOXP3
T15922 31998-32007 NN denotes induction
T15923 32008-32010 IN denotes in
T15924 32011-32016 JJ denotes human
T15925 32016-32017 , denotes ,
T15926 32018-32033 JJ denotes differentiating
T15927 32033-32034 , denotes ,
T15928 32035-32040 JJ denotes naive
T15929 32041-32042 NNP denotes T
T15930 32043-32048 NNS denotes cells
T15931 32048-32049 . denotes .
T15932 32050-32054 DT denotes This
T15933 32055-32065 JJ denotes inhibitory
T15934 32066-32072 NN denotes effect
T15935 32073-32075 IN denotes of
T15936 32076-32081 NNP denotes GATA3
T15937 32082-32085 VBD denotes was
T15938 32086-32093 JJ denotes further
T15939 32094-32103 VBN denotes confirmed
T15940 32104-32106 IN denotes in
T15941 32107-32113 NNP denotes BALB/c
T15942 32114-32124 JJ denotes transgenic
T15943 32125-32129 NNS denotes mice
T15944 32129-32130 , denotes ,
T15945 32131-32141 VBG denotes expressing
T15946 32142-32147 NNP denotes GATA3
T15947 32148-32150 IN denotes in
T15948 32151-32152 NNP denotes T
T15949 32153-32158 NNS denotes cells
T15950 32159-32160 -LRB- denotes (
T15951 32160-32164 CD denotes DO11
T15952 32164-32167 CD denotes .10
T15953 32167-32168 : denotes :
T15954 32168-32177 JJ denotes CD2-GATA3
T15955 32178-32188 JJ denotes transgenic
T15956 32189-32193 NNS denotes mice
T15957 32193-32194 -RRB- denotes )
T15958 32194-32195 . denotes .
T15959 32196-32198 IN denotes In
T15960 32199-32203 NN denotes line
T15961 32204-32208 IN denotes with
T15962 32209-32212 DT denotes the
T15963 32213-32222 NN denotes transient
T15964 32223-32237 NN denotes overexpression
T15965 32238-32240 IN denotes of
T15966 32241-32246 NNP denotes GATA3
T15967 32247-32249 IN denotes in
T15968 32250-32255 JJ denotes human
T15969 32256-32257 NNP denotes T
T15970 32258-32263 NNS denotes cells
T15971 32263-32264 , denotes ,
T15972 32265-32270 NNS denotes cells
T15973 32271-32273 IN denotes of
T15974 32274-32279 DT denotes these
T15975 32280-32284 NNS denotes mice
T15976 32285-32291 VBD denotes failed
T15977 32292-32294 TO denotes to
T15978 32295-32301 VB denotes induce
T15979 32302-32307 CD denotes FOXP3
T15980 32308-32318 NN denotes expression
T15981 32319-32323 IN denotes upon
T15982 32324-32332 NN denotes exposure
T15983 32333-32337 IN denotes with
T15984 32338-32345 NN denotes antigen
T15985 32346-32348 IN denotes in
T15986 32349-32352 DT denotes the
T15987 32353-32361 NN denotes presence
T15988 32362-32364 IN denotes of
T15989 32365-32370 NNP denotes TGF-β
T15990 32370-32371 . denotes .
T15991 32372-32382 RB denotes Strikingly
T15992 32382-32383 , denotes ,
T15993 32384-32387 DT denotes the
T15994 32388-32392 NNP denotes DO11
T15995 32392-32395 CD denotes .10
T15996 32395-32396 : denotes :
T15997 32396-32405 JJ denotes CD2-GATA3
T15998 32406-32410 NNS denotes mice
T15999 32411-32413 VBP denotes do
T16000 32414-32418 VB denotes have
T16001 32419-32429 JJ denotes peripheral
T16002 32430-32435 CD denotes FOXP3
T16003 32435-32436 NN denotes +
T16004 32437-32442 NNS denotes cells
T16005 32442-32443 , denotes ,
T16006 32444-32449 WDT denotes which
T16007 32450-32457 RB denotes however
T16008 32458-32467 VBD denotes displayed
T16009 32468-32469 DT denotes a
T16010 32470-32472 CD denotes 10
T16011 32472-32473 NN denotes %
T16012 32474-32476 CD denotes 25
T16013 32476-32477 NN denotes %
T16014 32478-32483 JJR denotes lower
T16015 32484-32493 NN denotes frequency
T16016 32494-32502 VBN denotes compared
T16017 32503-32505 TO denotes to
T16018 32506-32515 JJ denotes wild-type
T16019 32516-32520 NNP denotes DO11
T16020 32520-32523 CD denotes .10
T16021 32524-32528 NNS denotes mice
T16022 32528-32529 . denotes .
T16023 32530-32534 RB denotes Thus
T16024 32535-32540 JJ denotes GATA3
T16025 32541-32550 NNS denotes restrains
T16026 32551-32554 DT denotes the
T16027 32555-32566 NN denotes development
T16028 32567-32569 IN denotes of
T16029 32570-32577 JJ denotes certain
T16030 32578-32582 NNP denotes Treg
T16031 32583-32590 NNS denotes subsets
T16032 32590-32591 , denotes ,
T16033 32592-32602 RB denotes presumably
T16034 32603-32606 DT denotes the
T16035 32607-32616 JJ denotes inducible
T16036 32616-32617 , denotes ,
T16037 32618-32628 JJ denotes peripheral
T16038 32629-32639 NN denotes population
T16039 32640-32643 CC denotes and
T16040 32644-32647 RB denotes not
T16041 32648-32653 DT denotes those
T16042 32654-32656 IN denotes of
T16043 32657-32663 JJ denotes thymic
T16044 32664-32670 NN denotes origin
T16045 32670-32671 . denotes .
T16046 32672-32678 NNP denotes Thymic
T16047 32679-32680 NNP denotes T
T16048 32681-32686 NNS denotes cells
T16049 32687-32694 VBP denotes undergo
T16050 32695-32696 DT denotes a
T16051 32697-32706 JJ denotes different
T16052 32707-32717 NN denotes maturation
T16053 32718-32725 NN denotes process
T16054 32725-32726 , denotes ,
T16055 32727-32732 WDT denotes which
T16056 32733-32736 MD denotes may
T16057 32737-32744 VB denotes explain
T16058 32745-32748 DT denotes the
T16059 32749-32762 NN denotes insensitivity
T16060 32763-32765 IN denotes of
T16061 32766-32771 NNP denotes nTreg
T16062 32772-32774 TO denotes to
T16063 32775-32780 NNP denotes GATA3
T16064 32781-32795 NN denotes overexpression
T16065 32796-32797 NNP denotes [
T16066 32797-32799 CD denotes 52
T16067 32799-32800 NNP denotes ]
T16068 32800-32801 . denotes .
T16069 32802-32804 IN denotes In
T16070 32805-32813 NN denotes contrast
T16071 32814-32816 TO denotes to
T16072 32817-32820 DT denotes the
T16073 32821-32840 NN denotes Th2-differentiating
T16074 32841-32844 CC denotes and
T16075 32845-32861 JJ denotes iTreg-inhibiting
T16076 32862-32870 NN denotes function
T16077 32871-32873 IN denotes of
T16078 32874-32879 NNP denotes GATA3
T16079 32880-32882 IN denotes in
T16080 32883-32893 JJ denotes peripheral
T16081 32894-32895 NNP denotes T
T16082 32896-32901 NNS denotes cells
T16083 32901-32902 , denotes ,
T16084 32903-32908 JJ denotes GATA3
T16085 32909-32913 NNS denotes acts
T16086 32914-32916 IN denotes in
T16087 32917-32920 DT denotes the
T16088 32921-32927 NN denotes thymus
T16089 32928-32936 RB denotes together
T16090 32937-32941 IN denotes with
T16091 32942-32947 JJ denotes other
T16092 32948-32961 NN denotes transcription
T16093 32962-32969 NNS denotes factors
T16094 32970-32974 JJ denotes such
T16095 32975-32977 IN denotes as
T16096 32978-32981 DT denotes the
T16097 32982-32991 NNP denotes Repressor
T16098 32992-32994 IN denotes of
T16099 32995-33000 NNP denotes GATA3
T16100 33001-33002 -LRB- denotes (
T16101 33002-33005 NNP denotes ROG
T16102 33005-33006 -RRB- denotes )
T16103 33007-33009 IN denotes in
T16104 33010-33013 DT denotes the
T16105 33014-33029 NN denotes differentiation
T16106 33030-33037 NN denotes process
T16107 33038-33044 IN denotes toward
T16108 33045-33048 NNP denotes CD8
T16109 33049-33054 NNS denotes cells
T16110 33055-33056 NNP denotes [
T16111 33056-33061 CD denotes 53,54
T16112 33061-33062 NNP denotes ]
T16113 33063-33065 CC denotes or
T16114 33066-33078 VBZ denotes participates
T16115 33079-33081 IN denotes in
T16116 33082-33089 JJ denotes complex
T16117 33090-33105 JJ denotes transcriptional
T16118 33106-33114 NN denotes feedback
T16119 33115-33122 NN denotes network
T16120 33123-33125 TO denotes to
T16121 33126-33134 VB denotes regulate
T16122 33135-33150 JJ denotes sympathoadrenal
T16123 33151-33166 NN denotes differentiation
T16124 33167-33168 NNP denotes [
T16125 33168-33170 CD denotes 55
T16126 33170-33171 NNP denotes ]
T16127 33171-33172 . denotes .
T16128 33173-33182 RB denotes Therefore
T16129 33182-33183 , denotes ,
T16130 33184-33187 DT denotes the
T16131 33188-33192 NN denotes role
T16132 33193-33195 IN denotes of
T16133 33196-33201 NNP denotes GATA3
T16134 33202-33209 VBZ denotes appears
T16135 33210-33212 TO denotes to
T16136 33213-33215 VB denotes be
T16137 33216-33222 NN denotes tissue
T16138 33223-33231 NN denotes specific
T16139 33232-33235 CC denotes and
T16140 33236-33239 MD denotes can
T16141 33239-33242 RB denotes not
T16142 33243-33245 VB denotes be
T16143 33246-33257 VBN denotes generalized
T16144 33257-33258 . denotes .
T16145 33259-33262 PRP$ denotes Our
T16146 33263-33268 NN denotes study
T16147 33269-33281 VBZ denotes demonstrates
T16148 33282-33286 IN denotes that
T16149 33287-33292 NNP denotes GATA3
T16150 33293-33302 VBD denotes repressed
T16151 33303-33308 CD denotes FOXP3
T16152 33309-33319 NN denotes expression
T16153 33320-33328 RB denotes directly
T16154 33329-33331 IN denotes by
T16155 33332-33339 JJ denotes binding
T16156 33340-33342 TO denotes to
T16157 33343-33346 DT denotes the
T16158 33347-33352 CD denotes FOXP3
T16159 33353-33361 NN denotes promoter
T16160 33362-33368 NN denotes region
T16161 33368-33369 . denotes .
T16162 33370-33371 DT denotes A
T16163 33372-33383 JJ denotes palindromic
T16164 33384-33393 JJ denotes GATA-site
T16165 33394-33396 VBZ denotes is
T16166 33397-33404 VBN denotes located
T16167 33405-33408 CD denotes 303
T16168 33409-33411 NN denotes bp
T16169 33412-33420 RB denotes upstream
T16170 33421-33423 IN denotes of
T16171 33424-33427 DT denotes the
T16172 33428-33431 NNP denotes TSS
T16173 33432-33434 IN denotes in
T16174 33435-33436 DT denotes a
T16175 33437-33443 RB denotes highly
T16176 33444-33453 VBN denotes conserved
T16177 33454-33460 NN denotes region
T16178 33460-33461 , denotes ,
T16179 33462-33467 WDT denotes which
T16180 33468-33470 PRP denotes we
T16181 33471-33475 VBP denotes have
T16182 33476-33486 RB denotes previously
T16183 33487-33497 VBN denotes identified
T16184 33498-33500 IN denotes as
T16185 33501-33504 DT denotes the
T16186 33505-33510 CD denotes FOXP3
T16187 33511-33519 NN denotes promoter
T16188 33520-33521 NNP denotes [
T16189 33521-33523 CD denotes 24
T16190 33523-33524 NNP denotes ]
T16191 33524-33525 . denotes .
T16192 33526-33539 JJ denotes Site-specific
T16193 33540-33548 NN denotes mutation
T16194 33549-33551 IN denotes of
T16195 33552-33556 DT denotes this
T16196 33557-33561 NN denotes site
T16197 33562-33571 VBD denotes increased
T16198 33572-33575 DT denotes the
T16199 33576-33584 NN denotes activity
T16200 33585-33587 IN denotes of
T16201 33588-33591 DT denotes the
T16202 33592-33600 NN denotes promoter
T16203 33601-33611 NNS denotes constructs
T16204 33611-33612 , denotes ,
T16205 33613-33617 RB denotes thus
T16206 33618-33627 VBG denotes revealing
T16207 33628-33631 DT denotes the
T16208 33632-33642 JJ denotes repressive
T16209 33643-33649 NN denotes nature
T16210 33650-33652 IN denotes of
T16211 33653-33657 DT denotes this
T16212 33658-33662 NNP denotes GATA
T16213 33663-33670 NN denotes element
T16214 33671-33673 IN denotes in
T16215 33674-33680 NN denotes memory
T16216 33681-33682 NNP denotes T
T16217 33683-33688 NNS denotes cells
T16218 33688-33689 , denotes ,
T16219 33690-33695 WDT denotes which
T16220 33696-33705 RB denotes naturally
T16221 33706-33713 VBP denotes express
T16222 33714-33719 NNP denotes GATA3
T16223 33719-33720 , denotes ,
T16224 33721-33728 IN denotes whereas
T16225 33729-33731 DT denotes no
T16226 33732-33742 NN denotes difference
T16227 33743-33746 VBD denotes was
T16228 33747-33751 VBN denotes seen
T16229 33752-33754 IN denotes in
T16230 33755-33760 JJ denotes naive
T16231 33761-33762 NNP denotes T
T16232 33763-33768 NNS denotes cells
T16233 33768-33769 , denotes ,
T16234 33770-33775 WDT denotes which
T16235 33776-33778 VBP denotes do
T16236 33779-33782 RB denotes not
T16237 33783-33790 VB denotes express
T16238 33791-33796 NNP denotes GATA3
T16239 33796-33797 . denotes .
T16240 33798-33802 DT denotes This
T16241 33803-33814 JJ denotes palindromic
T16242 33815-33819 NNP denotes GATA
T16243 33820-33827 NN denotes element
T16244 33828-33830 VBZ denotes is
T16245 33831-33836 VBN denotes bound
T16246 33837-33839 IN denotes by
T16247 33840-33845 NNP denotes GATA3
T16248 33846-33853 NN denotes protein
T16249 33854-33856 IN denotes as
T16250 33857-33863 VBN denotes proven
T16251 33864-33868 IN denotes with
T16252 33869-33878 JJ denotes pull-down
T16253 33879-33890 NNS denotes experiments
T16254 33890-33891 . denotes .
T16255 33892-33903 RB denotes Furthermore
T16256 33903-33904 , denotes ,
T16257 33905-33907 PRP denotes it
T16258 33908-33910 VBZ denotes is
T16259 33911-33916 VBN denotes shown
T16260 33917-33919 IN denotes by
T16261 33920-33924 NN denotes ChIP
T16262 33925-33929 IN denotes that
T16263 33930-33935 CD denotes GATA3
T16264 33936-33941 NNS denotes binds
T16265 33942-33946 DT denotes this
T16266 33947-33954 NN denotes element
T16267 33955-33959 RB denotes also
T16268 33960-33962 IN denotes in
T16269 33963-33969 JJ denotes intact
T16270 33970-33975 NNS denotes cells
T16271 33975-33976 . denotes .
T16272 33977-33979 PRP denotes It
T16273 33980-33982 VBZ denotes is
T16274 33983-33988 VBN denotes known
T16275 33989-33993 IN denotes that
T16276 33994-33999 NNP denotes GATA3
T16277 34000-34003 MD denotes can
T16278 34004-34010 VB denotes induce
T16279 34011-34024 NN denotes transcription
T16280 34025-34027 IN denotes by
T16281 34028-34037 NN denotes chromatin
T16282 34038-34048 VBG denotes remodeling
T16283 34049-34050 NNP denotes [
T16284 34050-34052 CD denotes 56
T16285 34052-34053 NNP denotes ]
T16286 34053-34054 , denotes ,
T16287 34055-34057 IN denotes by
T16288 34058-34066 RB denotes directly
T16289 34067-34082 VBG denotes transactivating
T16290 34083-34092 NNS denotes promoters
T16291 34093-34094 NNP denotes [
T16292 34094-34096 CD denotes 36
T16293 34096-34097 NNP denotes ]
T16294 34097-34098 , denotes ,
T16295 34099-34101 CC denotes or
T16296 34101-34102 , denotes ,
T16297 34103-34105 IN denotes as
T16298 34106-34111 VBN denotes shown
T16299 34112-34114 IN denotes in
T16300 34115-34118 DT denotes the
T16301 34119-34126 JJ denotes current
T16302 34127-34132 NN denotes study
T16303 34132-34133 , denotes ,
T16304 34134-34138 VBZ denotes acts
T16305 34139-34141 IN denotes as
T16306 34142-34143 DT denotes a
T16307 34144-34153 NN denotes repressor
T16308 34154-34156 IN denotes of
T16309 34157-34161 NN denotes gene
T16310 34162-34172 NN denotes expression
T16311 34173-34174 NNP denotes [
T16312 34174-34179 CD denotes 21,57
T16313 34180-34182 CD denotes 59
T16314 34182-34183 NN denotes ]
T16315 34183-34184 . denotes .
T16316 34185-34194 RB denotes Therefore
T16317 34195-34202 VBG denotes keeping
T16318 34203-34208 JJ denotes GATA3
T16319 34209-34219 NN denotes expression
T16320 34220-34223 JJ denotes low
T16321 34224-34229 MD denotes might
T16322 34230-34232 VB denotes be
T16323 34233-34241 VBN denotes required
T16324 34242-34244 TO denotes to
T16325 34245-34251 VB denotes induce
T16326 34252-34261 JJ denotes efficient
T16327 34262-34267 CD denotes FOXP3
T16328 34267-34268 CD denotes +
T16329 34269-34274 NNP denotes iTreg
T16330 34275-34279 NN denotes cell
T16331 34280-34290 NN denotes generation
T16332 34290-34291 . denotes .
T16333 34292-34295 DT denotes The
T16334 34296-34305 JJ denotes molecular
T16335 34306-34318 NNS denotes interactions
T16336 34319-34327 VBG denotes enabling
T16337 34328-34333 CD denotes GATA3
T16338 34334-34336 TO denotes to
T16339 34337-34344 VB denotes inhibit
T16340 34345-34350 CD denotes FOXP3
T16341 34351-34354 VBP denotes are
T16342 34355-34358 RB denotes not
T16343 34359-34369 VBN denotes identified
T16344 34370-34373 RB denotes yet
T16345 34373-34374 , denotes ,
T16346 34375-34378 CC denotes but
T16347 34379-34382 DT denotes the
T16348 34383-34395 JJ denotes GATA-binding
T16349 34396-34400 NN denotes site
T16350 34401-34403 VBZ denotes is
T16351 34404-34411 VBN denotes located
T16352 34412-34420 JJ denotes adjacent
T16353 34421-34423 TO denotes to
T16354 34424-34432 JJ denotes positive
T16355 34432-34433 , denotes ,
T16356 34434-34442 VBG denotes inducing
T16357 34443-34448 NNS denotes sites
T16358 34448-34449 , denotes ,
T16359 34450-34458 VBN denotes composed
T16360 34459-34461 IN denotes of
T16361 34462-34473 JJ denotes AP-1-NFATc2
T16362 34474-34479 NNS denotes sites
T16363 34480-34481 NNP denotes [
T16364 34481-34483 CD denotes 24
T16365 34483-34484 NNP denotes ]
T16366 34484-34485 , denotes ,
T16367 34486-34489 CC denotes and
T16368 34490-34495 NNP denotes GATA3
T16369 34496-34499 MD denotes may
T16370 34500-34507 VB denotes compete
T16371 34508-34512 IN denotes with
T16372 34513-34516 DT denotes the
T16373 34517-34524 JJ denotes binding
T16374 34525-34527 IN denotes of
T16375 34528-34532 NNP denotes AP-1
T16376 34532-34533 NNP denotes /
T16377 34533-34537 NNP denotes NFAT
T16378 34538-34540 TO denotes to
T16379 34541-34544 DT denotes the
T16380 34545-34553 NN denotes promoter
T16381 34554-34555 -LRB- denotes (
T16382 34555-34566 JJ denotes unpublished
T16383 34567-34579 NNS denotes observations
T16384 34579-34580 -RRB- denotes )
T16385 34580-34581 . denotes .
T16386 34582-34584 IN denotes In
T16387 34585-34592 NN denotes summary
T16388 34592-34593 , denotes ,
T16389 34594-34596 PRP denotes we
T16390 34597-34609 VBD denotes demonstrated
T16391 34610-34614 IN denotes that
T16392 34615-34620 CD denotes FOXP3
T16393 34621-34623 VBZ denotes is
T16394 34624-34634 RB denotes negatively
T16395 34635-34644 VBN denotes regulated
T16396 34645-34647 IN denotes by
T16397 34648-34657 NNS denotes cytokines
T16398 34658-34662 JJ denotes such
T16399 34663-34665 IN denotes as
T16400 34666-34670 NN denotes IL-4
T16401 34670-34671 . denotes .
T16402 34672-34677 CD denotes GATA3
T16403 34678-34682 NNS denotes acts
T16404 34683-34685 IN denotes as
T16405 34686-34688 DT denotes an
T16406 34689-34698 NN denotes inhibitor
T16407 34699-34701 IN denotes of
T16408 34702-34707 CD denotes FOXP3
T16409 34708-34718 NN denotes expression
T16410 34719-34721 IN denotes in
T16411 34722-34727 JJ denotes early
T16412 34728-34729 NNP denotes T
T16413 34730-34734 NN denotes cell
T16414 34735-34750 NN denotes differentiation
T16415 34750-34751 , denotes ,
T16416 34752-34754 RB denotes as
T16417 34755-34759 RB denotes well
T16418 34760-34762 IN denotes as
T16419 34763-34765 IN denotes in
T16420 34766-34780 JJ denotes differentiated
T16421 34781-34784 JJ denotes Th2
T16422 34785-34790 NNS denotes cells
T16423 34791-34793 IN denotes by
T16424 34794-34802 RB denotes directly
T16425 34803-34810 JJ denotes binding
T16426 34811-34814 CC denotes and
T16427 34815-34825 VBG denotes repressing
T16428 34826-34829 DT denotes the
T16429 34830-34835 CD denotes FOXP3
T16430 34836-34844 NN denotes promoter
T16431 34844-34845 . denotes .
T16432 34846-34848 PRP denotes We
T16433 34849-34858 RB denotes therefore
T16434 34859-34867 VBP denotes describe
T16435 34868-34869 DT denotes a
T16436 34870-34873 JJ denotes new
T16437 34874-34883 NN denotes mechanism
T16438 34884-34886 IN denotes of
T16439 34887-34890 WRB denotes how
T16440 34891-34895 JJ denotes Il-4
T16441 34896-34902 NNS denotes avoids
T16442 34903-34912 NN denotes tolerance
T16443 34913-34922 NN denotes induction
T16444 34923-34925 IN denotes by
T16445 34926-34936 VBG denotes repressing
T16446 34937-34942 CD denotes FOXP3
T16447 34943-34953 NN denotes expression
T16448 34953-34954 . denotes .
T16449 34955-34960 DT denotes These
T16450 34961-34969 NNS denotes findings
T16451 34970-34974 MD denotes will
T16452 34975-34979 VB denotes give
T16453 34980-34983 JJ denotes new
T16454 34984-34996 NNS denotes perspectives
T16455 34997-35003 IN denotes toward
T16456 35004-35017 VBG denotes understanding
T16457 35018-35027 JJ denotes molecular
T16458 35028-35038 NNS denotes mechanisms
T16459 35039-35041 IN denotes of
T16460 35042-35047 NNP denotes iTreg
T16461 35048-35057 NN denotes induction
T16462 35058-35061 CC denotes and
T16463 35062-35066 RB denotes thus
T16464 35067-35075 NNS denotes pathways
T16465 35076-35078 IN denotes of
T16466 35079-35089 JJ denotes peripheral
T16467 35090-35099 NN denotes tolerance
T16468 35100-35109 NN denotes induction
T16469 35109-35110 , denotes ,
T16470 35111-35123 RB denotes particularly
T16471 35124-35126 IN denotes in
T16472 35127-35134 NN denotes allergy
T16473 35135-35138 CC denotes and
T16474 35139-35145 NN denotes asthma
T16475 35145-35146 . denotes .
T17891 35171-35175 NNP denotes Mice
T17892 35175-35176 . denotes .
T17893 35177-35183 JJ denotes Normal
T17894 35184-35186 NN denotes B6
T17895 35187-35191 NNS denotes mice
T17896 35192-35196 VBD denotes were
T17897 35197-35206 VBN denotes purchased
T17898 35207-35211 IN denotes from
T17899 35212-35215 DT denotes the
T17900 35216-35223 NNP denotes Jackson
T17901 35224-35236 NNP denotes Laboratories
T17902 35237-35238 -LRB- denotes (
T17903 35238-35241 NNP denotes Bar
T17904 35242-35248 NNP denotes Harbor
T17905 35248-35249 , denotes ,
T17906 35250-35255 NNP denotes Maine
T17907 35255-35256 -RRB- denotes )
T17908 35256-35257 . denotes .
T17909 35258-35268 NNP denotes Transgenic
T17910 35269-35273 NNP denotes DO11
T17911 35273-35276 CD denotes .10
T17912 35277-35281 NNS denotes mice
T17913 35281-35282 , denotes ,
T17914 35283-35293 VBG denotes expressing
T17915 35294-35295 DT denotes a
T17916 35296-35297 NNP denotes T
T17917 35298-35302 NN denotes cell
T17918 35303-35311 NN denotes receptor
T17919 35312-35315 IN denotes for
T17920 35316-35322 NNP denotes OVA323
T17921 35323-35326 CD denotes 339
T17922 35327-35334 NN denotes peptide
T17923 35335-35337 IN denotes in
T17924 35338-35341 DT denotes the
T17925 35342-35349 NN denotes context
T17926 35350-35352 IN denotes of
T17927 35353-35357 JJ denotes H-2d
T17928 35357-35358 , denotes ,
T17929 35359-35363 VBD denotes were
T17930 35364-35375 VBN denotes backcrossed
T17931 35376-35380 IN denotes with
T17932 35381-35385 NNS denotes mice
T17933 35386-35396 VBG denotes expressing
T17934 35397-35403 NNP denotes GATA-3
T17935 35403-35404 , denotes ,
T17936 35405-35411 VBN denotes driven
T17937 35412-35414 IN denotes by
T17938 35415-35418 DT denotes the
T17939 35419-35424 JJ denotes human
T17940 35425-35428 NNP denotes CD2
T17941 35429-35434 NN denotes locus
T17942 35435-35442 NN denotes control
T17943 35443-35449 NN denotes region
T17944 35450-35451 -LRB- denotes (
T17945 35451-35460 NN denotes CD2-GATA3
T17946 35460-35461 -RRB- denotes )
T17947 35462-35463 NNP denotes [
T17948 35463-35465 CD denotes 22
T17949 35465-35466 NNP denotes ]
T17950 35466-35467 , denotes ,
T17951 35468-35477 VBG denotes resulting
T17952 35478-35480 IN denotes in
T17953 35481-35498 JJ denotes DO11.10xCD2-GATA3
T17954 35499-35503 NNS denotes mice
T17955 35503-35504 . denotes .
T17956 35505-35509 NN denotes Mice
T17957 35510-35514 VBN denotes used
T17958 35515-35518 IN denotes for
T17959 35519-35530 NNS denotes experiments
T17960 35531-35535 VBD denotes were
T17961 35536-35547 VBN denotes backcrossed
T17962 35548-35550 IN denotes on
T17963 35551-35552 DT denotes a
T17964 35553-35559 NNP denotes BALB/c
T17965 35560-35570 NN denotes background
T17966 35571-35574 IN denotes for
T17967 35575-35576 DT denotes a
T17968 35577-35584 NN denotes minimum
T17969 35585-35587 IN denotes of
T17970 35588-35593 CD denotes eight
T17971 35594-35605 NNS denotes generations
T17972 35606-35609 CC denotes and
T17973 35610-35614 VBN denotes used
T17974 35615-35617 IN denotes at
T17975 35618-35620 DT denotes an
T17976 35621-35624 NN denotes age
T17977 35625-35627 IN denotes of
T17978 35628-35629 CD denotes 8
T17979 35630-35632 CD denotes 12
T17980 35633-35635 NN denotes wk
T17981 35635-35636 . denotes .
T17982 35637-35641 NNS denotes Mice
T17983 35642-35646 VBD denotes were
T17984 35647-35653 VBN denotes housed
T17985 35654-35659 IN denotes under
T17986 35660-35668 JJ denotes specific
T17987 35669-35682 JJ denotes pathogen-free
T17988 35683-35693 NNS denotes conditions
T17989 35694-35697 CC denotes and
T17990 35698-35701 DT denotes all
T17991 35702-35708 JJ denotes animal
T17992 35709-35716 NNS denotes studies
T17993 35717-35721 VBD denotes were
T17994 35722-35731 VBN denotes performed
T17995 35732-35741 VBG denotes according
T17996 35742-35744 TO denotes to
T17997 35745-35758 JJ denotes institutional
T17998 35759-35762 CC denotes and
T17999 35763-35768 NN denotes state
T18000 35769-35779 NNS denotes guidelines
T18001 35779-35780 . denotes .
T18166 35782-35791 NN denotes Isolation
T18167 35792-35794 IN denotes of
T18168 35795-35798 CD denotes CD4
T18169 35798-35799 CD denotes +
T18170 35800-35801 NNP denotes T
T18171 35802-35807 NNS denotes cells
T18172 35807-35808 . denotes .
T18173 35809-35812 CD denotes CD4
T18174 35812-35813 CD denotes +
T18175 35814-35815 NNP denotes T
T18176 35816-35821 NNS denotes cells
T18177 35822-35826 VBD denotes were
T18178 35827-35835 VBN denotes isolated
T18179 35836-35840 IN denotes from
T18180 35841-35846 NN denotes blood
T18181 35847-35849 IN denotes of
T18182 35850-35857 JJ denotes healthy
T18183 35858-35863 JJ denotes human
T18184 35864-35874 NNS denotes volunteers
T18185 35875-35880 VBG denotes using
T18186 35881-35884 DT denotes the
T18187 35885-35893 JJ denotes anti-CD4
T18188 35894-35902 JJ denotes magnetic
T18189 35903-35908 NNS denotes beads
T18190 35909-35910 -LRB- denotes (
T18191 35910-35915 NNP denotes Dynal
T18192 35915-35916 , denotes ,
T18193 35917-35924 NNP denotes Hamburg
T18194 35924-35925 , denotes ,
T18195 35926-35933 NNP denotes Germany
T18196 35933-35934 -RRB- denotes )
T18197 35935-35937 IN denotes as
T18198 35938-35948 RB denotes previously
T18199 35949-35958 VBN denotes described
T18200 35959-35960 NNP denotes [
T18201 35960-35962 CD denotes 60
T18202 35962-35963 NNP denotes ]
T18203 35963-35964 . denotes .
T18204 35965-35968 DT denotes The
T18205 35969-35975 NN denotes purity
T18206 35976-35978 IN denotes of
T18207 35979-35982 CD denotes CD4
T18208 35982-35983 CD denotes +
T18209 35984-35985 NNP denotes T
T18210 35986-35991 NNS denotes cells
T18211 35992-35995 VBD denotes was
T18212 35996-36005 RB denotes initially
T18213 36006-36012 VBN denotes tested
T18214 36013-36015 IN denotes by
T18215 36016-36020 NNP denotes FACS
T18216 36021-36024 CC denotes and
T18217 36025-36028 VBD denotes was
T18218 36029-36030 VBN denotes ≥
T18219 36031-36033 CD denotes 95
T18220 36033-36034 NN denotes %
T18221 36034-36035 . denotes .
T18222 36036-36049 NN denotes Monoclonality
T18223 36050-36052 IN denotes of
T18224 36053-36054 NNP denotes T
T18225 36055-36059 NN denotes cell
T18226 36060-36066 NNS denotes clones
T18227 36067-36070 VBD denotes was
T18228 36071-36080 VBN denotes confirmed
T18229 36081-36083 IN denotes by
T18230 36084-36093 JJ denotes TCR-chain
T18231 36094-36101 NN denotes mapping
T18232 36102-36105 CC denotes and
T18233 36106-36109 VBD denotes was
T18234 36110-36120 VBN denotes identified
T18235 36121-36123 TO denotes to
T18236 36124-36126 VB denotes be
T18237 36127-36133 JJ denotes Vbeta8
T18238 36134-36142 JJ denotes positive
T18239 36142-36143 . denotes .
T18240 36144-36147 DT denotes The
T18241 36148-36154 NNS denotes clones
T18242 36155-36159 VBD denotes were
T18243 36160-36173 VBN denotes characterized
T18244 36174-36176 IN denotes by
T18245 36177-36181 JJ denotes high
T18246 36182-36186 NN denotes IL-4
T18247 36187-36196 NN denotes secretion
T18248 36196-36197 . denotes .
T18362 36199-36211 JJ denotes Quantitative
T18363 36212-36221 JJ denotes real-time
T18364 36222-36225 NNP denotes PCR
T18365 36225-36226 . denotes .
T18366 36227-36230 DT denotes The
T18367 36231-36234 NNP denotes PCR
T18368 36235-36242 NNS denotes primers
T18369 36243-36246 CC denotes and
T18370 36247-36253 NNS denotes probes
T18371 36254-36258 VBD denotes were
T18372 36259-36267 VBN denotes designed
T18373 36268-36273 VBN denotes based
T18374 36274-36276 IN denotes on
T18375 36277-36280 DT denotes the
T18376 36281-36290 NNS denotes sequences
T18377 36291-36299 VBD denotes reported
T18378 36300-36302 IN denotes in
T18379 36303-36310 NNP denotes GenBank
T18380 36311-36315 IN denotes with
T18381 36316-36319 DT denotes the
T18382 36320-36326 NNP denotes Primer
T18383 36327-36334 NNP denotes Express
T18384 36335-36343 NN denotes software
T18385 36344-36351 NN denotes version
T18386 36352-36355 CD denotes 1.2
T18387 36356-36357 -LRB- denotes (
T18388 36357-36364 NNP denotes Applied
T18389 36365-36375 NNPS denotes Biosystems
T18390 36375-36376 -RRB- denotes )
T18391 36377-36379 IN denotes as
T18392 36380-36387 VBZ denotes follows
T18393 36387-36388 : denotes :
T18394 36389-36394 CD denotes FOXP3
T18395 36395-36402 RB denotes forward
T18396 36403-36409 NN denotes primer
T18397 36410-36411 CD denotes 5
T18398 36411-36412 SYM denotes ′
T18399 36412-36413 : denotes -
T18400 36413-36416 NNP denotes GAA
T18401 36417-36420 NNP denotes ACA
T18402 36421-36424 NNP denotes GCA
T18403 36425-36428 NNP denotes CAT
T18404 36429-36432 NNP denotes TCC
T18405 36433-36436 NNP denotes CAG
T18406 36437-36440 NNP denotes AGT
T18407 36441-36445 JJ denotes TC-3
T18408 36445-36446 NN denotes ′
T18409 36446-36447 : denotes ;
T18410 36448-36453 CD denotes FOXP3
T18411 36454-36461 NN denotes reverse
T18412 36462-36468 NN denotes primer
T18413 36469-36470 CD denotes 5
T18414 36470-36471 SYM denotes ′
T18415 36471-36472 : denotes -
T18416 36472-36475 NNP denotes ATG
T18417 36476-36479 NNP denotes GCC
T18418 36480-36483 NNP denotes CAG
T18419 36484-36487 NNP denotes CGG
T18420 36488-36491 NNP denotes ATG
T18421 36492-36496 JJ denotes AG-3
T18422 36496-36497 NN denotes ′
T18423 36497-36498 : denotes ;
T18424 36499-36504 NNP denotes EF-1α
T18425 36505-36512 RB denotes forward
T18426 36513-36519 JJR denotes primer
T18427 36520-36523 CC denotes and
T18428 36524-36531 VB denotes reverse
T18429 36532-36538 NN denotes primer
T18430 36539-36541 IN denotes as
T18431 36542-36551 VBN denotes described
T18432 36552-36553 NNP denotes [
T18433 36553-36555 CD denotes 61
T18434 36555-36556 NNP denotes ]
T18435 36556-36557 : denotes ;
T18436 36558-36563 NNP denotes GATA3
T18437 36564-36571 RB denotes forward
T18438 36572-36578 JJR denotes primer
T18439 36579-36580 CD denotes 5
T18440 36580-36581 SYM denotes ′
T18441 36581-36582 : denotes -
T18442 36582-36585 NNP denotes GCG
T18443 36586-36589 NNP denotes GGC
T18444 36590-36593 NNP denotes TCT
T18445 36594-36597 NNP denotes ATC
T18446 36598-36601 NNP denotes ACA
T18447 36602-36605 NNP denotes AAA
T18448 36606-36611 CD denotes TGA-3
T18449 36611-36612 NN denotes ′
T18450 36613-36616 CC denotes and
T18451 36617-36620 NN denotes rwd
T18452 36621-36622 CD denotes 5
T18453 36622-36623 SYM denotes ′
T18454 36623-36624 : denotes -
T18455 36624-36627 NNP denotes GCT
T18456 36628-36631 NNP denotes CTC
T18457 36632-36635 NNP denotes CTG
T18458 36636-36639 NNP denotes GCT
T18459 36640-36643 NNP denotes GCA
T18460 36644-36647 NNP denotes GAC
T18461 36648-36653 JJ denotes AGC-3
T18462 36653-36654 NN denotes ′
T18463 36654-36655 . denotes .
T18464 36656-36659 DT denotes The
T18465 36660-36668 JJ denotes prepared
T18466 36669-36674 NNS denotes cDNAs
T18467 36675-36679 VBD denotes were
T18468 36680-36689 VBN denotes amplified
T18469 36690-36695 VBG denotes using
T18470 36696-36704 NNP denotes SYBR-PCR
T18471 36705-36714 NN denotes mastermix
T18472 36715-36716 -LRB- denotes (
T18473 36716-36722 NNP denotes Biorad
T18474 36722-36723 -RRB- denotes )
T18475 36724-36733 VBG denotes according
T18476 36734-36736 TO denotes to
T18477 36737-36740 DT denotes the
T18478 36741-36756 NNS denotes recommendations
T18479 36757-36759 IN denotes of
T18480 36760-36763 DT denotes the
T18481 36764-36776 NN denotes manufacturer
T18482 36777-36779 IN denotes in
T18483 36780-36782 DT denotes an
T18484 36783-36786 NNP denotes ABI
T18485 36787-36792 NNP denotes PRISM
T18486 36793-36797 CD denotes 7000
T18487 36798-36806 NNP denotes Sequence
T18488 36807-36816 NNP denotes Detection
T18489 36817-36823 NNP denotes System
T18490 36824-36825 -LRB- denotes (
T18491 36825-36832 NNP denotes Applied
T18492 36833-36843 NNPS denotes Biosystems
T18493 36843-36844 -RRB- denotes )
T18494 36844-36845 . denotes .
T18495 36846-36858 JJ denotes Quantitative
T18496 36859-36862 NNP denotes PCR
T18497 36863-36865 IN denotes of
T18498 36866-36872 NN denotes murine
T18499 36873-36880 NNS denotes samples
T18500 36881-36884 VBD denotes was
T18501 36885-36894 VBN denotes performed
T18502 36895-36899 IN denotes with
T18503 36900-36909 JJ denotes Brilliant
T18504 36910-36914 NNP denotes SYBR
T18505 36915-36920 NNP denotes Green
T18506 36921-36925 NNP denotes QPCR
T18507 36926-36932 NN denotes master
T18508 36933-36936 NN denotes mix
T18509 36937-36938 -LRB- denotes (
T18510 36938-36948 NNP denotes Stratagene
T18511 36948-36949 -RRB- denotes )
T18512 36950-36953 CC denotes and
T18513 36954-36957 DT denotes the
T18514 36958-36967 VBG denotes following
T18515 36968-36975 NNS denotes primers
T18516 36975-36976 : denotes :
T18517 36977-36986 NNP denotes Ubiquitin
T18518 36987-36988 NNP denotes C
T18519 36988-36989 , denotes ,
T18520 36990-36991 CD denotes 5
T18521 36991-36992 SYM denotes ′
T18522 36992-36993 : denotes -
T18523 36994-36997 NNP denotes AGG
T18524 36998-37001 NNP denotes TCA
T18525 37002-37005 NNP denotes AAC
T18526 37006-37009 NNP denotes AGG
T18527 37010-37013 NNP denotes AAG
T18528 37014-37017 NNP denotes ACA
T18529 37018-37021 NNP denotes GAC
T18530 37022-37027 NNP denotes GTA-3
T18531 37027-37028 NNP denotes ′
T18532 37029-37032 CC denotes and
T18533 37033-37034 CD denotes 5
T18534 37034-37035 SYM denotes ′
T18535 37035-37036 : denotes -
T18536 37036-37059 NN denotes TCACACCCAAGAACAAGCACA-3
T18537 37059-37060 NN denotes ′
T18538 37060-37061 : denotes ;
T18539 37062-37068 NNP denotes Smad-7
T18540 37068-37069 , denotes ,
T18541 37070-37071 CD denotes 5
T18542 37071-37072 SYM denotes ′
T18543 37072-37073 : denotes -
T18544 37073-37076 NNP denotes GAA
T18545 37077-37080 NNP denotes ACC
T18546 37081-37084 NNP denotes GGG
T18547 37085-37088 NNP denotes GGA
T18548 37089-37092 NNP denotes ACG
T18549 37093-37096 NNP denotes AAT
T18550 37097-37102 CD denotes TAT-3
T18551 37102-37103 NN denotes ′
T18552 37104-37107 CC denotes and
T18553 37108-37109 CD denotes 5
T18554 37109-37110 SYM denotes ′
T18555 37110-37111 : denotes -
T18556 37111-37114 NNP denotes CGC
T18557 37115-37118 NNP denotes GAG
T18558 37119-37122 NNP denotes TCT
T18559 37123-37126 NNP denotes TCT
T18560 37127-37130 NNP denotes CCT
T18561 37131-37134 NNP denotes CCC
T18562 37135-37138 JJ denotes A-3
T18563 37138-37139 NN denotes ′
T18564 37139-37140 : denotes ;
T18565 37141-37147 NNP denotes TGF-ß1
T18566 37147-37148 , denotes ,
T18567 37149-37150 CD denotes 5
T18568 37150-37151 SYM denotes ′
T18569 37151-37152 : denotes -
T18570 37152-37155 NNP denotes TGA
T18571 37156-37159 NNP denotes CGT
T18572 37160-37163 NNP denotes CAC
T18573 37164-37167 NNP denotes TGG
T18574 37168-37171 NNP denotes AGT
T18575 37172-37175 NNP denotes TGT
T18576 37176-37179 NNP denotes ACG
T18577 37180-37183 NNP denotes G-3
T18578 37183-37184 NNP denotes ′
T18579 37185-37188 CC denotes and
T18580 37189-37190 CD denotes 5
T18581 37190-37191 SYM denotes ′
T18582 37191-37192 : denotes -
T18583 37192-37195 NNP denotes GGT
T18584 37196-37199 NNP denotes TCA
T18585 37200-37203 NNP denotes TGT
T18586 37204-37207 NNP denotes CAT
T18587 37208-37211 NNP denotes GGA
T18588 37212-37215 NNP denotes TGG
T18589 37216-37221 CD denotes TGC-3
T18590 37221-37222 NN denotes ′
T18591 37222-37223 . denotes .
T18592 37224-37230 NNP denotes Primer
T18593 37231-37236 NNS denotes pairs
T18594 37237-37241 VBD denotes were
T18595 37242-37251 VBN denotes evaluated
T18596 37252-37255 IN denotes for
T18597 37256-37265 NN denotes integrity
T18598 37266-37268 IN denotes by
T18599 37269-37277 NN denotes analysis
T18600 37278-37280 IN denotes of
T18601 37281-37284 DT denotes the
T18602 37285-37298 NN denotes amplification
T18603 37299-37303 NN denotes plot
T18604 37303-37304 , denotes ,
T18605 37305-37317 NN denotes dissociation
T18606 37318-37324 NNS denotes curves
T18607 37324-37325 , denotes ,
T18608 37326-37329 CC denotes and
T18609 37330-37340 NN denotes efficiency
T18610 37341-37343 IN denotes of
T18611 37344-37347 NNP denotes PCR
T18612 37348-37361 NN denotes amplification
T18613 37361-37362 . denotes .
T18614 37363-37366 NNP denotes PCR
T18615 37367-37377 NNS denotes conditions
T18616 37378-37382 VBD denotes were
T18617 37383-37385 CD denotes 10
T18618 37386-37389 NN denotes min
T18619 37390-37392 IN denotes at
T18620 37393-37395 CD denotes 95
T18621 37396-37397 CD denotes °
T18622 37397-37398 NNP denotes C
T18623 37398-37399 , denotes ,
T18624 37400-37408 VBN denotes followed
T18625 37409-37411 IN denotes by
T18626 37412-37414 CD denotes 40
T18627 37415-37421 NNS denotes cycles
T18628 37422-37424 IN denotes of
T18629 37425-37427 CD denotes 15
T18630 37428-37429 PRP denotes s
T18631 37430-37432 IN denotes at
T18632 37433-37435 CD denotes 95
T18633 37436-37437 CD denotes °
T18634 37437-37438 NNP denotes C
T18635 37439-37442 CC denotes and
T18636 37443-37445 CD denotes 60
T18637 37446-37447 NN denotes °
T18638 37447-37448 NNP denotes C
T18639 37449-37452 IN denotes for
T18640 37453-37454 CD denotes 1
T18641 37455-37458 NN denotes min
T18642 37459-37464 VBG denotes using
T18643 37465-37467 DT denotes an
T18644 37468-37472 CD denotes 7300
T18645 37473-37482 JJ denotes real-time
T18646 37483-37486 NNP denotes PCR
T18647 37487-37493 NN denotes system
T18648 37494-37495 -LRB- denotes (
T18649 37495-37502 NNP denotes Applied
T18650 37503-37513 NNPS denotes Biosystems
T18651 37513-37514 -RRB- denotes )
T18652 37514-37515 . denotes .
T18653 37516-37519 NNP denotes PCR
T18654 37520-37533 NN denotes amplification
T18655 37534-37536 IN denotes of
T18656 37537-37540 DT denotes the
T18657 37541-37553 NN denotes housekeeping
T18658 37554-37558 NN denotes gene
T18659 37559-37567 VBG denotes encoding
T18660 37568-37577 NN denotes ubiquitin
T18661 37578-37579 NNP denotes C
T18662 37580-37583 VBD denotes was
T18663 37584-37593 VBN denotes performed
T18664 37594-37600 IN denotes during
T18665 37601-37605 DT denotes each
T18666 37606-37609 NN denotes run
T18667 37610-37613 IN denotes for
T18668 37614-37618 DT denotes each
T18669 37619-37625 NN denotes sample
T18670 37626-37628 TO denotes to
T18671 37629-37634 VB denotes allow
T18672 37635-37648 NN denotes normalization
T18673 37649-37656 IN denotes between
T18674 37657-37664 NNS denotes samples
T18675 37664-37665 . denotes .
T18676 37666-37674 JJ denotes Relative
T18677 37675-37689 NN denotes quantification
T18678 37690-37693 CC denotes and
T18679 37694-37705 NN denotes calculation
T18680 37706-37708 IN denotes of
T18681 37709-37712 DT denotes the
T18682 37713-37718 NN denotes range
T18683 37719-37721 IN denotes of
T18684 37722-37732 NN denotes confidence
T18685 37733-37736 VBD denotes was
T18686 37737-37746 VBN denotes performed
T18687 37747-37752 VBG denotes using
T18688 37753-37756 DT denotes the
T18689 37757-37768 JJ denotes comparative
T18690 37769-37773 NNP denotes ΔΔCT
T18691 37774-37780 NN denotes method
T18692 37780-37781 . denotes .
T21516 42878-42884 JJ denotes murine
T18896 37783-37792 JJ denotes Inducible
T18897 37793-37799 NN denotes murine
T18898 37800-37804 NNP denotes Treg
T18899 37805-37812 NN denotes culture
T18900 37812-37813 . denotes .
T18901 37814-37819 JJ denotes Naive
T18902 37820-37823 NNP denotes CD4
T18903 37823-37824 CD denotes +
T18904 37825-37826 NNP denotes T
T18905 37827-37832 NNS denotes cells
T18906 37833-37834 -LRB- denotes (
T18907 37834-37837 CD denotes CD4
T18908 37837-37838 NN denotes +
T18909 37838-37839 , denotes ,
T18910 37840-37845 NNP denotes CD62L
T18911 37845-37846 NNP denotes +
T18912 37846-37847 , denotes ,
T18913 37848-37851 CC denotes and
T18914 37852-37856 NNP denotes CD25
T18915 37857-37858 -RRB- denotes )
T18916 37859-37863 VBD denotes were
T18917 37864-37872 VBN denotes isolated
T18918 37873-37877 IN denotes from
T18919 37878-37884 VBN denotes pooled
T18920 37885-37890 NN denotes lymph
T18921 37891-37896 NNS denotes nodes
T18922 37897-37900 CC denotes and
T18923 37901-37908 NNS denotes spleens
T18924 37909-37911 IN denotes by
T18925 37912-37916 NNP denotes FACS
T18926 37917-37918 -LRB- denotes (
T18927 37918-37922 NNP denotes FACS
T18928 37923-37927 NNP denotes Aria
T18929 37927-37928 , denotes ,
T18930 37929-37931 NNP denotes BD
T18931 37932-37943 NNP denotes Biosciences
T18932 37943-37944 -RRB- denotes )
T18933 37944-37945 . denotes .
T18934 37946-37947 CD denotes 5
T18935 37948-37949 NN denotes ×
T18936 37950-37953 CD denotes 105
T18937 37954-37955 NNP denotes T
T18938 37956-37961 NNS denotes cells
T18939 37962-37966 VBD denotes were
T18940 37967-37978 VBN denotes co-cultured
T18941 37979-37983 IN denotes with
T18942 37984-37987 CD denotes 2.5
T18943 37988-37989 CD denotes ×
T18944 37990-37993 CD denotes 104
T18945 37994-37998 NN denotes bone
T18946 37999-38005 NN denotes marrow
T18947 38006-38013 VBN denotes derived
T18948 38014-38023 JJ denotes dendritic
T18949 38024-38029 NNS denotes cells
T18950 38030-38031 NNP denotes [
T18951 38031-38033 CD denotes 62
T18952 38033-38034 NNP denotes ]
T18953 38035-38038 CC denotes and
T18954 38039-38043 CD denotes 0.01
T18955 38044-38046 NN denotes μg
T18956 38046-38047 NN denotes /
T18957 38047-38049 NN denotes ml
T18958 38050-38056 NNP denotes OVA323
T18959 38057-38060 CD denotes 339
T18960 38061-38068 NN denotes peptide
T18961 38069-38070 -LRB- denotes (
T18962 38070-38077 NNP denotes Ansynth
T18963 38077-38078 -RRB- denotes )
T18964 38079-38081 IN denotes in
T18965 38082-38085 DT denotes the
T18966 38086-38094 NN denotes presence
T18967 38095-38097 CC denotes or
T18968 38098-38105 NN denotes absence
T18969 38106-38108 IN denotes of
T18970 38109-38114 JJ denotes 20-ng
T18971 38114-38115 NN denotes /
T18972 38115-38117 NN denotes μl
T18973 38118-38126 NN denotes rhTGF-ß1
T18974 38127-38128 -LRB- denotes (
T18975 38128-38137 NNP denotes Peprotech
T18976 38137-38138 -RRB- denotes )
T18977 38139-38141 IN denotes in
T18978 38142-38149 JJ denotes 48-well
T18979 38150-38156 NNS denotes plates
T18980 38156-38157 . denotes .
T18981 38158-38163 IN denotes After
T18982 38164-38165 CD denotes 4
T18983 38166-38167 LS denotes d
T18984 38167-38168 , denotes ,
T18985 38169-38174 NNS denotes cells
T18986 38175-38179 VBD denotes were
T18987 38180-38189 VBN denotes harvested
T18988 38190-38193 CC denotes and
T18989 38194-38202 VBN denotes analyzed
T18990 38203-38206 IN denotes for
T18991 38207-38220 JJ denotes intracellular
T18992 38221-38226 CD denotes FOXP3
T18993 38227-38237 NN denotes expression
T18994 38238-38240 IN denotes by
T18995 38241-38245 NNP denotes FACS
T18996 38246-38248 CC denotes or
T18997 38249-38253 NN denotes gene
T18998 38254-38264 NN denotes expression
T18999 38265-38267 IN denotes by
T19000 38268-38280 JJ denotes quantitative
T19001 38281-38287 NN denotes RT-PCR
T19002 38287-38288 . denotes .
T19255 38290-38292 IN denotes In
T19256 38293-38298 NN denotes vitro
T19257 38299-38300 NNP denotes T
T19258 38301-38305 NN denotes cell
T19259 38306-38321 NN denotes differentiation
T19260 38321-38322 . denotes .
T19261 38323-38326 CD denotes CD4
T19262 38326-38327 CD denotes +
T19263 38328-38334 NNP denotes CD45RA
T19264 38334-38335 NNP denotes +
T19265 38336-38355 JJ denotes magnetically-sorted
T19266 38356-38357 -LRB- denotes (
T19267 38357-38363 CD denotes CD45RO
T19268 38364-38373 NN denotes depletion
T19269 38373-38374 , denotes ,
T19270 38375-38379 NNP denotes MACS
T19271 38379-38380 , denotes ,
T19272 38381-38390 VBG denotes according
T19273 38391-38393 TO denotes to
T19274 38394-38397 DT denotes the
T19275 38398-38406 NN denotes protocol
T19276 38407-38409 IN denotes of
T19277 38410-38413 DT denotes the
T19278 38414-38426 NN denotes manufacturer
T19279 38426-38427 -RRB- denotes )
T19280 38428-38433 NNS denotes cells
T19281 38434-38438 VBD denotes were
T19282 38439-38449 VBN denotes stimulated
T19283 38450-38454 IN denotes with
T19284 38455-38466 JJ denotes immobilized
T19285 38467-38478 JJ denotes plate-bound
T19286 38479-38487 JJ denotes anti-CD3
T19287 38488-38489 -LRB- denotes (
T19288 38489-38490 CD denotes 1
T19289 38491-38493 NN denotes μg
T19290 38493-38494 NN denotes /
T19291 38494-38496 NN denotes ml
T19292 38496-38497 , denotes ,
T19293 38498-38502 NNP denotes Okt3
T19294 38502-38503 , denotes ,
T19295 38504-38508 NNP denotes IgG1
T19296 38508-38509 -RRB- denotes )
T19297 38510-38513 CC denotes and
T19298 38514-38523 JJ denotes anti-CD28
T19299 38524-38525 -LRB- denotes (
T19300 38525-38526 CD denotes 2
T19301 38527-38529 NN denotes μg
T19302 38529-38530 NN denotes /
T19303 38530-38532 NN denotes ml
T19304 38532-38533 -RRB- denotes )
T19305 38534-38536 IN denotes in
T19306 38537-38540 JJ denotes Th1
T19307 38541-38551 NNS denotes conditions
T19308 38551-38552 : denotes :
T19309 38553-38555 CD denotes 25
T19310 38556-38561 NN denotes ng/ml
T19311 38562-38567 NN denotes IL-12
T19312 38567-38568 , denotes ,
T19313 38569-38570 CD denotes 5
T19314 38571-38573 NN denotes μg
T19315 38573-38574 NN denotes /
T19316 38574-38576 NN denotes ml
T19317 38577-38586 NN denotes anti-IL-4
T19318 38587-38588 -LRB- denotes (
T19319 38588-38591 NNP denotes R&D
T19320 38592-38599 NNS denotes systems
T19321 38599-38600 -RRB- denotes )
T19322 38600-38601 : denotes ;
T19323 38602-38604 IN denotes in
T19324 38605-38608 JJ denotes Th2
T19325 38609-38619 NNS denotes conditions
T19326 38619-38620 : denotes :
T19327 38621-38623 CD denotes 25
T19328 38624-38629 NN denotes ng/ml
T19329 38630-38634 NN denotes IL-4
T19330 38634-38635 , denotes ,
T19331 38636-38637 CD denotes 5
T19332 38638-38640 NN denotes μg
T19333 38640-38641 NN denotes /
T19334 38641-38643 NN denotes ml
T19335 38644-38654 JJ denotes anti-IFN-γ
T19336 38654-38655 , denotes ,
T19337 38656-38657 CD denotes 5
T19338 38658-38660 NN denotes μg
T19339 38660-38661 NN denotes /
T19340 38661-38663 NN denotes ml
T19341 38664-38674 NN denotes anti-IL-12
T19342 38675-38676 -LRB- denotes (
T19343 38676-38679 NNP denotes R&D
T19344 38680-38687 NNS denotes systems
T19345 38687-38688 -RRB- denotes )
T19346 38688-38689 : denotes ;
T19347 38690-38692 CC denotes or
T19348 38693-38695 IN denotes in
T19349 38696-38700 NNP denotes Treg
T19350 38701-38711 NNS denotes conditions
T19351 38711-38712 : denotes :
T19352 38713-38715 CD denotes 10
T19353 38716-38721 NN denotes ng/ml
T19354 38722-38727 NNP denotes TGF-β
T19355 38727-38728 , denotes ,
T19356 38729-38730 CD denotes 5
T19357 38731-38733 NN denotes μg
T19358 38733-38734 NN denotes /
T19359 38734-38736 NN denotes ml
T19360 38737-38747 JJ denotes anti-IFN-γ
T19361 38747-38748 , denotes ,
T19362 38749-38750 CD denotes 5
T19363 38751-38753 NN denotes μg
T19364 38753-38754 NN denotes /
T19365 38754-38756 NN denotes ml
T19366 38757-38767 NN denotes anti-IL-12
T19367 38767-38768 , denotes ,
T19368 38769-38770 CD denotes 5
T19369 38771-38773 NN denotes μg
T19370 38773-38774 NN denotes /
T19371 38774-38776 NN denotes ml
T19372 38777-38786 NN denotes anti-IL-4
T19373 38786-38787 . denotes .
T19374 38788-38801 VBG denotes Proliferating
T19375 38802-38807 NNS denotes cells
T19376 38808-38812 VBD denotes were
T19377 38813-38821 VBN denotes expanded
T19378 38822-38824 IN denotes in
T19379 38825-38831 JJ denotes medium
T19380 38832-38842 VBG denotes containing
T19381 38843-38847 NN denotes IL-2
T19382 38848-38849 -LRB- denotes (
T19383 38849-38851 CD denotes 30
T19384 38852-38857 NN denotes ng/ml
T19385 38857-38858 -RRB- denotes )
T19386 38858-38859 . denotes .
T19618 38861-38868 VBG denotes Cloning
T19619 38869-38871 IN denotes of
T19620 38872-38875 DT denotes the
T19621 38876-38881 CD denotes FOXP3
T19622 38882-38890 NN denotes promoter
T19623 38891-38894 CC denotes and
T19624 38895-38907 NN denotes construction
T19625 38908-38910 IN denotes of
T19626 38911-38917 JJ denotes mutant
T19627 38918-38928 NNS denotes constructs
T19628 38928-38929 . denotes .
T19629 38930-38933 DT denotes The
T19630 38934-38939 CD denotes FOXP3
T19631 38940-38948 NN denotes promoter
T19632 38949-38952 VBD denotes was
T19633 38953-38959 VBN denotes cloned
T19634 38960-38964 IN denotes into
T19635 38965-38968 DT denotes the
T19636 38969-38973 JJ denotes pGL3
T19637 38974-38979 JJ denotes basic
T19638 38980-38986 NN denotes vector
T19639 38987-38988 -LRB- denotes (
T19640 38988-38995 NNP denotes Promega
T19641 38996-39003 NNP denotes Biotech
T19642 39003-39004 -RRB- denotes )
T19643 39005-39007 TO denotes to
T19644 39008-39016 VB denotes generate
T19645 39017-39020 DT denotes the
T19646 39021-39025 JJ denotes pGL3
T19647 39026-39031 CD denotes FOXP3
T19648 39032-39033 CD denotes −
T19649 39033-39036 CD denotes 511
T19650 39036-39037 NN denotes /
T19651 39037-39041 CD denotes +176
T19652 39042-39043 NNP denotes [
T19653 39043-39045 CD denotes 24
T19654 39045-39046 NNP denotes ]
T19655 39046-39047 . denotes .
T19656 39048-39061 JJ denotes Site-directed
T19657 39062-39073 NNS denotes mutagenesis
T19658 39074-39076 IN denotes in
T19659 39077-39080 DT denotes the
T19660 39081-39086 CD denotes FOXP3
T19661 39087-39095 NN denotes promoter
T19662 39096-39102 NN denotes region
T19663 39103-39107 VBD denotes were
T19664 39108-39118 VBN denotes introduced
T19665 39119-39124 VBG denotes using
T19666 39125-39128 DT denotes the
T19667 39129-39140 NNP denotes QuickChange
T19668 39141-39144 NN denotes kit
T19669 39145-39146 -LRB- denotes (
T19670 39146-39156 NNP denotes Stratagene
T19671 39156-39157 -RRB- denotes )
T19672 39157-39158 , denotes ,
T19673 39159-39168 VBG denotes according
T19674 39169-39171 TO denotes to
T19675 39172-39175 DT denotes the
T19676 39176-39188 NN denotes manufacturer
T19677 39188-39190 POS denotes 's
T19678 39191-39203 NNS denotes instructions
T19679 39203-39204 . denotes .
T19680 39205-39208 DT denotes The
T19681 39209-39218 JJ denotes following
T19682 39219-39225 NN denotes primer
T19683 39226-39229 CC denotes and
T19684 39230-39233 PRP$ denotes its
T19685 39234-39247 JJ denotes complementary
T19686 39248-39254 NN denotes strand
T19687 39255-39259 VBD denotes were
T19688 39260-39264 VBN denotes used
T19689 39264-39265 : denotes :
T19690 39266-39269 NNP denotes GTT
T19691 39270-39273 NNP denotes TCT
T19692 39274-39277 NNP denotes CAT
T19693 39278-39281 NNP denotes GAG
T19694 39282-39285 NNP denotes CCC
T19695 39286-39289 NNP denotes TAT
T19696 39290-39293 NNP denotes TAA
T19697 39294-39297 NNP denotes GTC
T19698 39298-39301 NNP denotes ATT
T19699 39302-39305 NNP denotes CTT
T19700 39306-39309 NNP denotes ACC
T19701 39310-39313 NNP denotes TCT
T19702 39314-39317 NNP denotes CAC
T19703 39318-39321 NNP denotes CTC
T19704 39322-39325 NNP denotes TGT
T19705 39326-39329 NNP denotes GGT
T19706 39330-39333 NNP denotes GA.
T19885 39335-39348 NNS denotes Transfections
T19886 39349-39352 CC denotes and
T19887 39353-39361 NN denotes reporter
T19888 39362-39366 NN denotes gene
T19889 39367-39373 NNS denotes assays
T19890 39373-39374 . denotes .
T19891 39375-39376 NNP denotes T
T19892 39377-39382 NNS denotes cells
T19893 39383-39387 VBD denotes were
T19894 39388-39394 VBN denotes rested
T19895 39395-39397 IN denotes in
T19896 39398-39408 JJ denotes serum-free
T19897 39409-39414 NNP denotes AIM-V
T19898 39415-39421 NN denotes medium
T19899 39422-39423 -LRB- denotes (
T19900 39423-39427 NNP denotes Life
T19901 39428-39440 NNPS denotes Technologies
T19902 39440-39441 -RRB- denotes )
T19903 39442-39451 RB denotes overnight
T19904 39451-39452 . denotes .
T19905 39453-39456 CD denotes 3.5
T19906 39457-39459 NN denotes μg
T19907 39460-39462 IN denotes of
T19908 39463-39466 DT denotes the
T19909 39467-39472 CD denotes FOXP3
T19910 39473-39481 NN denotes promoter
T19911 39482-39492 NN denotes luciferase
T19912 39493-39501 NN denotes reporter
T19913 39502-39508 NN denotes vector
T19914 39509-39512 CC denotes and
T19915 39513-39516 CD denotes 0.5
T19916 39517-39519 NN denotes μg
T19917 39520-39527 NN denotes phRL-TK
T19918 39528-39532 VBD denotes were
T19919 39533-39538 VBN denotes added
T19920 39539-39541 TO denotes to
T19921 39542-39543 CD denotes 3
T19922 39544-39545 CD denotes ×
T19923 39546-39549 CD denotes 106
T19924 39550-39553 NNP denotes CD4
T19925 39553-39554 CD denotes +
T19926 39555-39556 NNP denotes T
T19927 39557-39562 NNS denotes cells
T19928 39563-39574 VBD denotes resuspended
T19929 39575-39577 IN denotes in
T19930 39578-39581 CD denotes 100
T19931 39582-39584 NN denotes μL
T19932 39585-39587 IN denotes of
T19933 39588-39600 NNP denotes Nucleofector
T19934 39601-39609 NN denotes solution
T19935 39610-39611 -LRB- denotes (
T19936 39611-39616 NNP denotes Amaxa
T19937 39617-39627 NNPS denotes Biosystems
T19938 39627-39628 -RRB- denotes )
T19939 39629-39632 CC denotes and
T19940 39633-39647 VBD denotes electroporated
T19941 39648-39653 VBG denotes using
T19942 39654-39657 DT denotes the
T19943 39658-39662 NN denotes U-15
T19944 39663-39670 NN denotes program
T19945 39671-39673 IN denotes of
T19946 39674-39677 DT denotes the
T19947 39678-39690 NNP denotes Nucleofector
T19948 39690-39691 . denotes .
T19949 39692-39697 IN denotes After
T19950 39698-39699 DT denotes a
T19951 39700-39704 JJ denotes 24-h
T19952 39705-39712 NN denotes culture
T19953 39713-39715 IN denotes in
T19954 39716-39726 JJ denotes serum-free
T19955 39727-39737 NNS denotes conditions
T19956 39738-39741 CC denotes and
T19957 39742-39749 NNS denotes stimuli
T19958 39750-39752 IN denotes as
T19959 39753-39762 VBN denotes indicated
T19960 39763-39765 IN denotes in
T19961 39766-39769 DT denotes the
T19962 39770-39777 NNS denotes figures
T19963 39777-39778 , denotes ,
T19964 39779-39789 NN denotes luciferase
T19965 39790-39798 NN denotes activity
T19966 39799-39802 VBD denotes was
T19967 39803-39811 VBN denotes measured
T19968 39812-39814 IN denotes by
T19969 39815-39818 DT denotes the
T19970 39819-39823 JJ denotes dual
T19971 39824-39834 NN denotes luciferase
T19972 39835-39840 NN denotes assay
T19973 39841-39847 NN denotes system
T19974 39848-39849 -LRB- denotes (
T19975 39849-39856 NNP denotes Promega
T19976 39857-39864 NNP denotes Biotech
T19977 39864-39865 -RRB- denotes )
T19978 39866-39875 VBG denotes according
T19979 39876-39878 TO denotes to
T19980 39879-39882 DT denotes the
T19981 39883-39895 NN denotes manufacturer
T19982 39895-39897 POS denotes 's
T19983 39898-39910 NNS denotes instructions
T19984 39910-39911 . denotes .
T19985 39912-39916 NNS denotes Data
T19986 39917-39921 VBD denotes were
T19987 39922-39932 VBN denotes normalized
T19988 39933-39935 IN denotes by
T19989 39936-39939 DT denotes the
T19990 39940-39948 NN denotes activity
T19991 39949-39951 IN denotes of
T19992 39952-39959 NN denotes renilla
T19993 39960-39970 NN denotes luciferase
T19994 39970-39971 . denotes .
T20100 39973-39976 NNP denotes RNA
T20101 39977-39986 NN denotes isolation
T20102 39987-39990 CC denotes and
T20103 39991-39995 NNP denotes cDNA
T20104 39996-40005 NN denotes synthesis
T20105 40005-40006 . denotes .
T20106 40007-40010 NNP denotes RNA
T20107 40011-40014 VBD denotes was
T20108 40015-40023 VBN denotes isolated
T20109 40024-40029 VBG denotes using
T20110 40030-40033 DT denotes the
T20111 40034-40040 NNP denotes RNeasy
T20112 40041-40045 NNP denotes Mini
T20113 40046-40049 NNP denotes Kit
T20114 40050-40051 -LRB- denotes (
T20115 40051-40057 NNP denotes Qiagen
T20116 40057-40058 -RRB- denotes )
T20117 40059-40068 VBG denotes according
T20118 40069-40071 TO denotes to
T20119 40072-40075 DT denotes the
T20120 40076-40088 NN denotes manufacturer
T20121 40088-40090 POS denotes 's
T20122 40091-40099 NN denotes protocol
T20123 40099-40100 . denotes .
T20124 40101-40108 VB denotes Reverse
T20125 40109-40122 NN denotes transcription
T20126 40123-40125 IN denotes of
T20127 40126-40131 JJ denotes human
T20128 40132-40139 NNS denotes samples
T20129 40140-40143 VBD denotes was
T20130 40144-40153 VBN denotes performed
T20131 40154-40158 IN denotes with
T20132 40159-40165 NNP denotes TaqMan
T20133 40166-40187 NN denotes reverse-transcription
T20134 40188-40196 NNS denotes reagents
T20135 40197-40198 -LRB- denotes (
T20136 40198-40205 NNP denotes Applied
T20137 40206-40216 NNPS denotes Biosystems
T20138 40216-40217 -RRB- denotes )
T20139 40218-40222 IN denotes with
T20140 40223-40229 JJ denotes random
T20141 40230-40238 NNS denotes hexamers
T20142 40239-40248 VBG denotes according
T20143 40249-40251 TO denotes to
T20144 40252-40255 DT denotes the
T20145 40256-40268 NN denotes manufacturer
T20146 40268-40270 POS denotes 's
T20147 40271-40279 NN denotes protocol
T20148 40279-40280 . denotes .
T20272 40282-40293 JJ denotes Recombinant
T20273 40294-40297 NNP denotes TAT
T20274 40298-40306 NNS denotes proteins
T20275 40306-40307 . denotes .
T20276 40308-40311 DT denotes The
T20277 40312-40317 NNP denotes cDNAs
T20278 40318-40326 VBG denotes encoding
T20279 40327-40332 CD denotes GATA3
T20280 40333-40340 NN denotes protein
T20281 40341-40343 CC denotes or
T20282 40344-40347 DT denotes the
T20283 40348-40357 VBN denotes truncated
T20284 40358-40363 NNP denotes GATA3
T20285 40364-40365 -LRB- denotes (
T20286 40365-40372 VBG denotes lacking
T20287 40373-40376 DT denotes the
T20288 40377-40380 CD denotes two
T20289 40381-40385 NN denotes zinc
T20290 40386-40393 NNS denotes fingers
T20291 40393-40394 -RRB- denotes )
T20292 40395-40399 VBD denotes were
T20293 40400-40406 VBN denotes cloned
T20294 40407-40409 IN denotes in
T20295 40410-40415 NN denotes frame
T20296 40416-40420 IN denotes into
T20297 40421-40423 DT denotes an
T20298 40424-40434 NN denotes expression
T20299 40435-40441 NN denotes vector
T20300 40442-40447 IN denotes along
T20301 40448-40452 IN denotes with
T20302 40453-40456 DT denotes the
T20303 40457-40460 NNP denotes TAT
T20304 40461-40469 NN denotes sequence
T20305 40470-40472 IN denotes as
T20306 40473-40483 RB denotes previously
T20307 40484-40493 VBN denotes described
T20308 40494-40495 NNP denotes [
T20309 40495-40497 CD denotes 63
T20310 40497-40498 NNP denotes ]
T20311 40498-40499 . denotes .
T20312 40500-40508 NNPS denotes Proteins
T20313 40509-40513 VBD denotes were
T20314 40514-40523 VBN denotes expressed
T20315 40524-40526 IN denotes in
T20316 40527-40531 NNP denotes BL21
T20317 40532-40536 NNP denotes Star
T20318 40537-40538 -LRB- denotes (
T20319 40538-40541 NNP denotes DE3
T20320 40541-40542 -RRB- denotes )
T20321 40542-40547 NNP denotes pLysS
T20322 40548-40549 -LRB- denotes (
T20323 40549-40559 NNP denotes Invitrogen
T20324 40559-40560 -RRB- denotes )
T20325 40561-40564 CC denotes and
T20326 40565-40572 NNS denotes lysates
T20327 40573-40577 VBD denotes were
T20328 40578-40586 VBN denotes purified
T20329 40587-40589 IN denotes by
T20330 40590-40593 NNP denotes Ni2
T20331 40593-40594 SYM denotes +
T20332 40594-40595 : denotes -
T20333 40595-40602 NN denotes chelate
T20334 40603-40609 NN denotes column
T20335 40610-40624 NN denotes chromatography
T20336 40624-40625 . denotes .
T20337 40626-40630 DT denotes Both
T20338 40631-40641 JJ denotes TAT-linked
T20339 40642-40650 NNS denotes proteins
T20340 40651-40655 VBD denotes were
T20341 40656-40660 JJR denotes more
T20342 40661-40665 IN denotes than
T20343 40666-40668 CD denotes 95
T20344 40668-40669 NN denotes %
T20345 40670-40674 JJ denotes pure
T20346 40674-40675 , denotes ,
T20347 40676-40681 VBN denotes based
T20348 40682-40684 IN denotes on
T20349 40685-40694 NNP denotes Coomassie
T20350 40695-40699 JJ denotes blue
T20351 40700-40708 NN denotes staining
T20352 40709-40711 IN denotes of
T20353 40712-40718 NN denotes sodium
T20354 40719-40728 NN denotes disulfate
T20355 40729-40739 NN denotes acrylamide
T20356 40740-40744 NNS denotes gels
T20357 40744-40745 . denotes .
T20544 40747-40756 CD denotes TAT-GATA3
T20545 40757-40769 NN denotes transduction
T20546 40769-40770 . denotes .
T20547 40771-40774 CD denotes CD4
T20548 40774-40775 CD denotes +
T20549 40775-40781 NNP denotes CD45RA
T20550 40781-40782 NN denotes +
T20551 40783-40788 NNS denotes cells
T20552 40789-40793 VBD denotes were
T20553 40794-40802 VBN denotes cultured
T20554 40803-40805 IN denotes in
T20555 40806-40810 NNP denotes AIMV
T20556 40811-40817 NN denotes medium
T20557 40818-40821 CC denotes and
T20558 40822-40832 VBN denotes transduced
T20559 40833-40837 IN denotes with
T20560 40838-40840 CD denotes 20
T20561 40841-40843 NNP denotes nM
T20562 40843-40844 , denotes ,
T20563 40845-40847 CD denotes 10
T20564 40848-40850 NN denotes nM
T20565 40850-40851 , denotes ,
T20566 40852-40854 CC denotes or
T20567 40855-40858 CD denotes 500
T20568 40859-40861 NN denotes nM
T20569 40862-40864 IN denotes of
T20570 40865-40876 JJ denotes full-length
T20571 40877-40879 CC denotes or
T20572 40880-40889 VBN denotes truncated
T20573 40890-40895 NNP denotes GATA3
T20574 40896-40900 IN denotes over
T20575 40901-40904 DT denotes the
T20576 40905-40911 NN denotes course
T20577 40912-40914 IN denotes of
T20578 40915-40916 CD denotes 4
T20579 40917-40918 NN denotes h
T20580 40918-40919 . denotes .
T20581 40920-40925 IN denotes After
T20582 40926-40927 CD denotes 4
T20583 40928-40929 NN denotes h
T20584 40929-40930 , denotes ,
T20585 40931-40934 DT denotes the
T20586 40935-40940 NNS denotes cells
T20587 40941-40945 VBD denotes were
T20588 40946-40952 VBN denotes washed
T20589 40953-40956 CC denotes and
T20590 40957-40966 VBN denotes activated
T20591 40967-40971 IN denotes with
T20592 40972-40979 JJ denotes soluble
T20593 40980-40988 JJ denotes anti-CD3
T20594 40989-40992 CC denotes and
T20595 40993-41002 JJ denotes anti-CD28
T20596 41003-41006 CC denotes and
T20597 41007-41012 JJ denotes TGF-β
T20598 41013-41014 -LRB- denotes (
T20599 41014-41016 CD denotes 10
T20600 41017-41022 NN denotes ng/ml
T20601 41022-41023 -RRB- denotes )
T20602 41023-41024 . denotes .
T20603 41025-41029 DT denotes Each
T20604 41030-41033 NN denotes day
T20605 41033-41034 , denotes ,
T20606 41035-41038 DT denotes the
T20607 41039-41042 NNP denotes TAT
T20608 41043-41051 NNS denotes proteins
T20609 41052-41056 VBD denotes were
T20610 41057-41064 RB denotes freshly
T20611 41065-41070 VBN denotes added
T20612 41071-41073 TO denotes to
T20613 41074-41077 DT denotes the
T20614 41078-41084 NN denotes medium
T20615 41084-41085 . denotes .
T20616 41086-41091 CD denotes FOXP3
T20617 41092-41102 NN denotes expression
T20618 41103-41106 VBD denotes was
T20619 41107-41115 VBN denotes measured
T20620 41116-41121 IN denotes after
T20621 41122-41123 CD denotes 5
T20622 41124-41125 LS denotes d
T20623 41126-41128 IN denotes by
T20624 41129-41142 JJ denotes intracellular
T20625 41143-41151 NN denotes staining
T20626 41151-41152 . denotes .
T20794 41154-41167 JJ denotes Intracellular
T20795 41168-41176 NN denotes cytokine
T20796 41177-41185 NN denotes staining
T20797 41185-41186 . denotes .
T20798 41187-41188 NNP denotes T
T20799 41189-41194 NNS denotes cells
T20800 41195-41199 VBD denotes were
T20801 41200-41210 VBN denotes stimulated
T20802 41211-41215 IN denotes with
T20803 41216-41217 CD denotes 2
T20804 41218-41219 CD denotes ×
T20805 41220-41222 CD denotes 10
T20806 41222-41223 NN denotes −
T20807 41223-41224 CD denotes 7
T20808 41225-41226 NNP denotes M
T20809 41227-41230 NNP denotes PMA
T20810 41231-41234 CC denotes and
T20811 41235-41236 CD denotes 1
T20812 41237-41239 NN denotes μg
T20813 41239-41240 NN denotes /
T20814 41240-41242 NN denotes ml
T20815 41243-41245 IN denotes of
T20816 41246-41255 NN denotes ionomycin
T20817 41256-41257 -LRB- denotes (
T20818 41257-41262 NNP denotes Sigma
T20819 41263-41272 NNPS denotes Chemicals
T20820 41272-41273 -RRB- denotes )
T20821 41274-41277 IN denotes for
T20822 41278-41279 CD denotes 4
T20823 41280-41281 NN denotes h
T20824 41281-41282 . denotes .
T20825 41283-41286 DT denotes The
T20826 41287-41296 JJ denotes following
T20827 41297-41300 NNP denotes mAb
T20828 41301-41304 VBD denotes was
T20829 41305-41309 VBN denotes used
T20830 41309-41310 : denotes :
T20831 41311-41323 JJ denotes anti-IL-4-PE
T20832 41324-41325 -LRB- denotes (
T20833 41325-41328 CD denotes 8D4
T20834 41329-41330 CD denotes 8
T20835 41330-41331 , denotes ,
T20836 41332-41334 NNP denotes BD
T20837 41334-41335 -RRB- denotes )
T20838 41335-41336 . denotes .
T20839 41337-41344 VBN denotes Matched
T20840 41345-41352 NN denotes isotype
T20841 41353-41361 NNS denotes controls
T20842 41362-41366 VBD denotes were
T20843 41367-41371 VBN denotes used
T20844 41372-41374 IN denotes at
T20845 41375-41378 DT denotes the
T20846 41379-41383 JJ denotes same
T20847 41384-41391 NN denotes protein
T20848 41392-41405 NN denotes concentration
T20849 41406-41408 IN denotes as
T20850 41409-41412 DT denotes the
T20851 41413-41423 JJ denotes respective
T20852 41424-41434 NNS denotes antibodies
T20853 41434-41435 . denotes .
T20854 41436-41446 JJ denotes Four-color
T20855 41447-41451 NNP denotes FACS
T20856 41452-41455 VBD denotes was
T20857 41456-41465 VBN denotes performed
T20858 41466-41471 VBG denotes using
T20859 41472-41474 DT denotes an
T20860 41475-41480 NNP denotes EPICS
T20861 41481-41487 NNP denotes XL-MCL
T20862 41488-41489 -LRB- denotes (
T20863 41489-41496 NNP denotes Beckman
T20864 41497-41504 NNP denotes Coulter
T20865 41504-41505 -RRB- denotes )
T20866 41506-41511 VBG denotes using
T20867 41512-41515 DT denotes the
T20868 41516-41524 NN denotes software
T20869 41525-41531 NNP denotes Expo32
T20870 41532-41539 NN denotes version
T20871 41540-41543 IN denotes for
T20872 41544-41548 NNS denotes data
T20873 41549-41560 NN denotes acquisition
T20874 41561-41564 CC denotes and
T20875 41565-41575 NN denotes evaluation
T20876 41575-41576 . denotes .
T21273 41578-41582 NN denotes Flow
T21274 41583-41592 NN denotes cytometry
T21275 41592-41593 . denotes .
T21276 41594-41597 IN denotes For
T21277 41598-41606 NN denotes analysis
T21278 41607-41609 IN denotes of
T21279 41610-41615 CD denotes FOXP3
T21280 41616-41626 NN denotes expression
T21281 41627-41629 IN denotes at
T21282 41630-41633 DT denotes the
T21283 41634-41645 JJ denotes single-cell
T21284 41646-41651 NN denotes level
T21285 41651-41652 , denotes ,
T21286 41653-41658 NNS denotes cells
T21287 41659-41663 VBD denotes were
T21288 41664-41669 JJ denotes first
T21289 41670-41677 VBN denotes stained
T21290 41678-41682 IN denotes with
T21291 41683-41686 DT denotes the
T21292 41687-41697 JJ denotes monoclonal
T21293 41698-41706 NN denotes antibody
T21294 41707-41711 NNS denotes CD25
T21295 41712-41713 -LRB- denotes (
T21296 41713-41720 NNP denotes Beckman
T21297 41721-41728 NNP denotes Coulter
T21298 41728-41729 -RRB- denotes )
T21299 41729-41730 , denotes ,
T21300 41731-41734 CC denotes and
T21301 41735-41740 IN denotes after
T21302 41741-41749 NN denotes fixation
T21303 41750-41753 CC denotes and
T21304 41754-41770 NN denotes permeabilization
T21305 41770-41771 , denotes ,
T21306 41772-41777 NNS denotes cells
T21307 41778-41782 VBD denotes were
T21308 41783-41792 VBN denotes incubated
T21309 41793-41797 IN denotes with
T21310 41798-41811 JJ denotes PE-conjugated
T21311 41812-41822 JJ denotes monoclonal
T21312 41823-41831 NN denotes antibody
T21313 41832-41838 NNP denotes PCH101
T21314 41839-41840 -LRB- denotes (
T21315 41840-41850 JJ denotes anti-human
T21316 41851-41856 CD denotes FOXP3
T21317 41856-41857 : denotes ;
T21318 41858-41869 NNP denotes eBioscience
T21319 41869-41870 -RRB- denotes )
T21320 41871-41876 VBN denotes based
T21321 41877-41879 IN denotes on
T21322 41880-41883 DT denotes the
T21323 41884-41896 NN denotes manufacturer
T21324 41896-41898 POS denotes 's
T21325 41899-41914 NNS denotes recommendations
T21326 41915-41918 CC denotes and
T21327 41919-41928 VBN denotes subjected
T21328 41929-41931 TO denotes to
T21329 41932-41936 NNP denotes FACS
T21330 41937-41938 -LRB- denotes (
T21331 41938-41943 NNP denotes EPICS
T21332 41944-41950 NNP denotes XL-MCL
T21333 41950-41951 -RRB- denotes )
T21334 41951-41952 . denotes .
T21335 41953-41956 IN denotes For
T21336 41957-41961 NN denotes cell
T21337 41962-41969 NN denotes surface
T21338 41970-41976 NN denotes marker
T21339 41977-41985 NN denotes staining
T21340 41985-41986 , denotes ,
T21341 41987-41992 NNS denotes cells
T21342 41993-41997 VBD denotes were
T21343 41998-42007 VBN denotes incubated
T21344 42008-42011 IN denotes for
T21345 42012-42014 CD denotes 20
T21346 42015-42018 NN denotes min
T21347 42019-42021 IN denotes at
T21348 42022-42023 CD denotes 4
T21349 42023-42024 CD denotes °
T21350 42024-42025 NNP denotes C
T21351 42026-42028 IN denotes in
T21352 42029-42037 VBG denotes staining
T21353 42038-42044 NN denotes buffer
T21354 42045-42049 IN denotes with
T21355 42050-42053 DT denotes the
T21356 42054-42063 VBG denotes following
T21357 42064-42074 NNS denotes antibodies
T21358 42074-42075 : denotes :
T21359 42076-42080 NNS denotes anti
T21360 42081-42089 NN denotes CD152-PE
T21361 42090-42091 -LRB- denotes (
T21362 42091-42097 NN denotes CTLA-4
T21363 42097-42098 : denotes ;
T21364 42099-42101 NNP denotes BD
T21365 42101-42102 -RRB- denotes )
T21366 42102-42103 , denotes ,
T21367 42104-42108 NNS denotes anti
T21368 42109-42113 NN denotes PD-1
T21369 42114-42115 -LRB- denotes (
T21370 42115-42127 NNS denotes eBiosciences
T21371 42127-42128 -RRB- denotes )
T21372 42128-42129 , denotes ,
T21373 42130-42139 JJ denotes anti-GITR
T21374 42140-42141 -LRB- denotes (
T21375 42141-42142 NN denotes R
T21376 42143-42144 CC denotes &
T21377 42145-42146 NNP denotes D
T21378 42147-42154 NNPS denotes Systems
T21379 42154-42155 -RRB- denotes )
T21380 42155-42156 , denotes ,
T21381 42157-42166 JJ denotes anti-CD69
T21382 42167-42168 -LRB- denotes (
T21383 42168-42175 NNP denotes Beckman
T21384 42176-42183 NNP denotes Coulter
T21385 42183-42184 -RRB- denotes )
T21386 42184-42185 , denotes ,
T21387 42186-42196 JJ denotes anti-CD103
T21388 42197-42198 -LRB- denotes (
T21389 42198-42212 NNP denotes DakoCytomation
T21390 42212-42213 -RRB- denotes )
T21391 42213-42214 , denotes ,
T21392 42215-42225 JJ denotes anti-CD62L
T21393 42226-42227 -LRB- denotes (
T21394 42227-42234 NNP denotes Beckman
T21395 42235-42242 NNP denotes Coulter
T21396 42242-42243 -RRB- denotes )
T21397 42243-42244 , denotes ,
T21398 42245-42247 CC denotes or
T21399 42248-42259 JJ denotes anti-HLA-DR
T21400 42260-42261 -LRB- denotes (
T21401 42261-42268 NNP denotes Beckman
T21402 42269-42276 NNP denotes Coulter
T21403 42276-42277 -RRB- denotes )
T21404 42277-42278 . denotes .
T21405 42279-42282 DT denotes The
T21406 42283-42291 NNS denotes controls
T21407 42292-42296 VBD denotes were
T21408 42297-42301 NNP denotes FITC
T21409 42301-42302 , denotes ,
T21410 42303-42305 NNP denotes PE
T21411 42305-42306 , denotes ,
T21412 42307-42309 CC denotes or
T21413 42310-42324 JJ denotes ECD-conjugated
T21414 42325-42330 NN denotes mouse
T21415 42331-42335 NN denotes IgG1
T21416 42336-42338 CC denotes or
T21417 42339-42342 NN denotes rat
T21418 42343-42348 NNS denotes IgG2a
T21419 42348-42349 . denotes .
T21420 42350-42353 IN denotes For
T21421 42354-42362 VBG denotes staining
T21422 42363-42365 IN denotes of
T21423 42366-42371 NN denotes mouse
T21424 42372-42377 NNS denotes cells
T21425 42377-42378 , denotes ,
T21426 42379-42382 DT denotes the
T21427 42383-42392 VBG denotes following
T21428 42393-42397 NNS denotes mAbs
T21429 42398-42402 IN denotes from
T21430 42403-42405 NNP denotes BD
T21431 42406-42417 NNPS denotes Biosciences
T21432 42418-42422 VBD denotes were
T21433 42423-42427 VBN denotes used
T21434 42428-42437 JJ denotes following
T21435 42438-42446 NN denotes standard
T21436 42447-42457 NNS denotes techniques
T21437 42458-42460 IN denotes as
T21438 42461-42470 VBN denotes described
T21439 42471-42476 IN denotes above
T21440 42476-42477 : denotes :
T21441 42478-42486 JJ denotes anti-CD3
T21442 42486-42487 , denotes ,
T21443 42488-42496 JJ denotes anti-CD4
T21444 42496-42497 , denotes ,
T21445 42498-42501 CC denotes and
T21446 42502-42511 NN denotes anti-CD25
T21447 42511-42512 . denotes .
T21448 42513-42524 NNP denotes Anti-FcγRII
T21449 42524-42525 NNP denotes /
T21450 42525-42528 NNP denotes III
T21451 42529-42537 NN denotes antibody
T21452 42538-42539 -LRB- denotes (
T21453 42539-42542 CD denotes 2.4
T21454 42542-42544 NNP denotes G2
T21455 42544-42545 , denotes ,
T21456 42546-42550 NNP denotes ATCC
T21457 42550-42551 -RRB- denotes )
T21458 42552-42555 VBD denotes was
T21459 42556-42564 VBN denotes included
T21460 42565-42567 IN denotes in
T21461 42568-42571 DT denotes all
T21462 42572-42581 NNS denotes stainings
T21463 42582-42584 TO denotes to
T21464 42585-42591 VB denotes reduce
T21465 42592-42603 JJ denotes nonspecific
T21466 42604-42612 NN denotes antibody
T21467 42613-42620 JJ denotes binding
T21468 42620-42621 . denotes .
T21469 42622-42624 TO denotes To
T21470 42625-42632 VB denotes isolate
T21471 42633-42638 JJ denotes naive
T21472 42639-42645 NN denotes murine
T21473 42646-42649 NNP denotes CD4
T21474 42650-42651 NNP denotes T
T21475 42652-42657 NNS denotes cells
T21476 42658-42662 IN denotes from
T21477 42663-42669 JJ denotes murine
T21478 42670-42674 NNP denotes DO11
T21479 42674-42677 CD denotes .10
T21480 42678-42680 CC denotes or
T21481 42681-42698 JJ denotes DO11.10xCD2-GATA3
T21482 42699-42700 NN denotes T
T21483 42701-42706 NNS denotes cells
T21484 42706-42707 , denotes ,
T21485 42708-42713 NNS denotes cells
T21486 42714-42718 VBD denotes were
T21487 42719-42726 VBN denotes stained
T21488 42727-42731 IN denotes with
T21489 42732-42746 JJ denotes anti-CD25-FITC
T21490 42746-42747 , denotes ,
T21491 42748-42761 JJ denotes anti-CD62L-PE
T21492 42761-42762 , denotes ,
T21493 42763-42766 CC denotes and
T21494 42767-42779 JJ denotes anti-CD4-APC
T21495 42780-42785 RB denotes prior
T21496 42786-42788 TO denotes to
T21497 42789-42796 VBG denotes sorting
T21498 42796-42797 . denotes .
T21499 42798-42802 NNP denotes Dead
T21500 42803-42808 NNS denotes cells
T21501 42809-42813 VBD denotes were
T21502 42814-42822 VBN denotes excluded
T21503 42823-42827 IN denotes with
T21504 42828-42829 CD denotes 4
T21505 42829-42830 NN denotes ′
T21506 42830-42831 '' denotes '
T21507 42831-42833 SYM denotes ,6
T21508 42833-42834 : denotes -
T21509 42834-42858 NN denotes Diamidino-2-phenylindole
T21510 42859-42860 -LRB- denotes (
T21511 42860-42864 NNP denotes DAPI
T21512 42864-42865 -RRB- denotes )
T21513 42865-42866 . denotes .
T21514 42867-42869 TO denotes To
T21515 42870-42877 VB denotes analyze
T21517 42885-42890 JJ denotes Foxp3
T21518 42891-42901 NN denotes expression
T21519 42902-42904 IN denotes in
T21520 42905-42914 JJ denotes inducible
T21521 42915-42919 NNP denotes Treg
T21522 42920-42928 NNS denotes cultures
T21523 42928-42929 , denotes ,
T21524 42930-42935 NNS denotes cells
T21525 42936-42940 VBD denotes were
T21526 42941-42948 VBN denotes stained
T21527 42949-42964 RB denotes intracellularly
T21528 42965-42969 IN denotes with
T21529 42970-42983 JJ denotes anti-Foxp3-PE
T21530 42984-42993 VBG denotes according
T21531 42994-42996 TO denotes to
T21532 42997-43009 NN denotes manufacturer
T21533 43009-43011 POS denotes 's
T21534 43012-43023 NN denotes instruction
T21535 43023-43024 , denotes ,
T21536 43025-43027 IN denotes in
T21537 43028-43039 NN denotes conjunction
T21538 43040-43044 IN denotes with
T21539 43045-43057 JJ denotes anti-CD4-APC
T21540 43058-43061 CC denotes and
T21541 43062-43071 NNP denotes LIVE/DEAD
T21542 43072-43079 JJ denotes fixable
T21543 43080-43084 JJ denotes dead
T21544 43085-43089 NN denotes cell
T21545 43090-43095 VB denotes stain
T21546 43096-43099 NN denotes kit
T21547 43100-43101 -LRB- denotes (
T21548 43101-43111 NNP denotes Invitrogen
T21549 43111-43112 -RRB- denotes )
T21550 43113-43115 TO denotes to
T21551 43116-43128 JJ denotes discriminate
T21552 43129-43133 JJ denotes live
T21553 43134-43139 NNS denotes cells
T21554 43139-43140 . denotes .
T21555 43141-43144 DT denotes All
T21556 43145-43155 JJ denotes monoclonal
T21557 43156-43166 NNS denotes antibodies
T21558 43167-43170 IN denotes for
T21559 43171-43177 JJ denotes murine
T21560 43178-43182 NN denotes cell
T21561 43183-43192 NNS denotes stainings
T21562 43193-43197 VBD denotes were
T21563 43198-43207 VBN denotes purchased
T21564 43208-43212 IN denotes from
T21565 43213-43224 NNP denotes eBioscience
T21566 43225-43227 CC denotes or
T21567 43228-43230 NNP denotes BD
T21568 43231-43242 NNPS denotes Biosciences
T21569 43242-43243 . denotes .
T21972 43245-43252 JJ denotes Western
T21973 43253-43261 VBG denotes blotting
T21974 43261-43262 . denotes .
T21975 43263-43266 IN denotes For
T21976 43267-43272 CD denotes FOXP3
T21977 43273-43281 NN denotes analysis
T21978 43282-43284 IN denotes on
T21979 43285-43288 DT denotes the
T21980 43289-43296 NN denotes protein
T21981 43297-43302 NN denotes level
T21982 43302-43303 , denotes ,
T21983 43304-43305 CD denotes 1
T21984 43306-43307 CD denotes ×
T21985 43308-43311 CD denotes 106
T21986 43312-43315 NNP denotes CD4
T21987 43315-43316 NNP denotes +
T21988 43316-43320 NNP denotes CD25
T21989 43322-43327 NNS denotes cells
T21990 43328-43332 VBD denotes were
T21991 43333-43338 VBN denotes lysed
T21992 43339-43342 CC denotes and
T21993 43343-43349 VBN denotes loaded
T21994 43350-43354 JJ denotes next
T21995 43355-43357 TO denotes to
T21996 43358-43359 DT denotes a
T21997 43360-43372 JJ denotes protein-mass
T21998 43373-43379 NN denotes ladder
T21999 43380-43381 -LRB- denotes (
T22000 43381-43390 NNP denotes Magicmark
T22001 43390-43391 , denotes ,
T22002 43392-43402 NNP denotes Invitrogen
T22003 43402-43403 -RRB- denotes )
T22004 43404-43406 IN denotes on
T22005 43407-43408 DT denotes a
T22006 43409-43415 NNP denotes NuPAGE
T22007 43416-43417 CD denotes 4
T22008 43418-43420 CD denotes 12
T22009 43420-43421 NN denotes %
T22010 43422-43430 JJ denotes bis-tris
T22011 43431-43434 NN denotes gel
T22012 43435-43436 -LRB- denotes (
T22013 43436-43446 NNP denotes Invitrogen
T22014 43446-43447 -RRB- denotes )
T22015 43447-43448 . denotes .
T22016 43449-43452 DT denotes The
T22017 43453-43461 NNS denotes proteins
T22018 43462-43466 VBD denotes were
T22019 43467-43481 VBN denotes electroblotted
T22020 43482-43486 IN denotes onto
T22021 43487-43488 DT denotes a
T22022 43489-43493 NNP denotes PVDF
T22023 43494-43502 NN denotes membrane
T22024 43503-43504 -LRB- denotes (
T22025 43504-43512 NNP denotes Amersham
T22026 43513-43517 NNP denotes Life
T22027 43518-43525 NNP denotes Science
T22028 43525-43526 -RRB- denotes )
T22029 43526-43527 . denotes .
T22030 43528-43538 JJ denotes Unspecific
T22031 43539-43546 JJ denotes binding
T22032 43547-43550 VBD denotes was
T22033 43551-43558 VBN denotes blocked
T22034 43559-43563 IN denotes with
T22035 43564-43567 NNP denotes BSA
T22036 43567-43568 , denotes ,
T22037 43569-43572 CC denotes and
T22038 43573-43576 DT denotes the
T22039 43577-43586 NNS denotes membranes
T22040 43587-43591 VBD denotes were
T22041 43592-43604 RB denotes subsequently
T22042 43605-43614 VBN denotes incubated
T22043 43615-43619 IN denotes with
T22044 43620-43622 DT denotes an
T22045 43623-43628 CD denotes 1:200
T22046 43629-43637 NN denotes dilution
T22047 43638-43640 IN denotes of
T22048 43641-43645 NN denotes goat
T22049 43646-43656 NN denotes anti-FOXP3
T22050 43657-43659 IN denotes in
T22051 43660-43668 VBG denotes blocking
T22052 43669-43675 NN denotes buffer
T22053 43676-43677 -LRB- denotes (
T22054 43677-43682 NNP denotes Abcam
T22055 43682-43683 -RRB- denotes )
T22056 43684-43693 RB denotes overnight
T22057 43694-43696 IN denotes at
T22058 43697-43698 CD denotes 4
T22059 43699-43700 CD denotes °
T22060 43700-43701 NNP denotes C
T22061 43701-43702 . denotes .
T22062 43703-43706 DT denotes The
T22063 43707-43712 NNS denotes blots
T22064 43713-43717 VBD denotes were
T22065 43718-43727 VBN denotes developed
T22066 43728-43733 VBG denotes using
T22067 43734-43736 DT denotes an
T22068 43737-43746 JJ denotes anti-goat
T22069 43747-43758 JJ denotes HRP-labeled
T22070 43759-43762 NNP denotes mAb
T22071 43763-43764 -LRB- denotes (
T22072 43764-43772 NNP denotes Amersham
T22073 43773-43784 NNPS denotes Biosciences
T22074 43784-43785 -RRB- denotes )
T22075 43786-43789 CC denotes and
T22076 43790-43800 VBN denotes visualized
T22077 43801-43805 IN denotes with
T22078 43806-43807 DT denotes a
T22079 43808-43811 NNP denotes LAS
T22080 43812-43816 CD denotes 1000
T22081 43817-43823 NN denotes camera
T22082 43824-43825 -LRB- denotes (
T22083 43825-43829 NNP denotes Fuji
T22084 43829-43830 -RRB- denotes )
T22085 43830-43831 . denotes .
T22086 43832-43841 NNS denotes Membranes
T22087 43842-43846 VBD denotes were
T22088 43847-43856 VBN denotes incubated
T22089 43857-43859 IN denotes in
T22090 43860-43869 VBG denotes stripping
T22091 43870-43876 NN denotes buffer
T22092 43877-43880 CC denotes and
T22093 43881-43891 JJ denotes re-blocked
T22094 43892-43895 IN denotes for
T22095 43896-43897 CD denotes 1
T22096 43898-43899 NN denotes h
T22097 43899-43900 . denotes .
T22098 43901-43904 DT denotes The
T22099 43905-43914 NNS denotes membranes
T22100 43915-43919 VBD denotes were
T22101 43920-43929 JJ denotes re-probed
T22102 43930-43935 VBG denotes using
T22103 43936-43946 JJ denotes anti-GATA3
T22104 43947-43948 -LRB- denotes (
T22105 43948-43951 CD denotes HG3
T22106 43952-43954 CD denotes 31
T22107 43954-43955 : denotes ;
T22108 43956-43961 NNP denotes Santa
T22109 43962-43966 NNP denotes Cruz
T22110 43967-43980 NNP denotes Biotechnology
T22111 43980-43981 -RRB- denotes )
T22112 43981-43982 , denotes ,
T22113 43983-43993 JJ denotes anti-T-bet
T22114 43994-43995 -LRB- denotes (
T22115 43995-43999 NNP denotes 4B10
T22116 43999-44000 , denotes ,
T22117 44001-44006 NNP denotes Santa
T22118 44007-44011 NNP denotes Cruz
T22119 44012-44025 NNP denotes Biotechnology
T22120 44025-44026 -RRB- denotes )
T22121 44026-44027 , denotes ,
T22122 44028-44038 JJ denotes anti-GAPDH
T22123 44039-44040 -LRB- denotes (
T22124 44040-44043 CD denotes 6C5
T22125 44043-44044 , denotes ,
T22126 44045-44051 NNP denotes Ambion
T22127 44051-44052 -RRB- denotes )
T22128 44052-44053 , denotes ,
T22129 44054-44072 JJ denotes anti-phospho-SMAD2
T22130 44073-44074 -LRB- denotes (
T22131 44074-44079 CD denotes 138D4
T22132 44079-44080 -RRB- denotes )
T22133 44080-44081 , denotes ,
T22134 44082-44100 JJ denotes anti-phospho-STAT6
T22135 44101-44102 -LRB- denotes (
T22136 44102-44105 NNP denotes 5A4
T22137 44105-44106 -RRB- denotes )
T22138 44106-44107 , denotes ,
T22139 44108-44111 CC denotes and
T22140 44112-44122 NNP denotes anti-STAT6
T22141 44123-44124 -LRB- denotes (
T22142 44124-44128 NNP denotes Cell
T22143 44129-44138 NNP denotes Signaling
T22144 44139-44149 NNP denotes Technology
T22145 44149-44150 -RRB- denotes )
T22146 44150-44151 , denotes ,
T22376 44153-44167 NNP denotes Administration
T22377 44168-44170 IN denotes of
T22378 44171-44180 NNS denotes cytokines
T22379 44181-44184 CC denotes and
T22380 44185-44195 NNS denotes antibodies
T22381 44196-44198 IN denotes in
T22382 44199-44203 NN denotes vivo
T22383 44203-44204 . denotes .
T22384 44205-44208 NNP denotes Age
T22385 44208-44209 : denotes -
T22386 44210-44213 CC denotes and
T22387 44214-44228 JJ denotes gender-matched
T22388 44229-44235 JJ denotes normal
T22389 44236-44238 NNP denotes B6
T22390 44239-44243 NNS denotes mice
T22391 44244-44252 VBD denotes received
T22392 44253-44258 DT denotes every
T22393 44259-44264 JJ denotes other
T22394 44265-44268 NN denotes day
T22395 44269-44284 JJ denotes intraperitoneal
T22396 44285-44286 -LRB- denotes (
T22397 44286-44288 NN denotes ip
T22398 44288-44289 -RRB- denotes )
T22399 44290-44300 NNS denotes injections
T22400 44301-44303 IN denotes of
T22401 44304-44307 NNP denotes PBS
T22402 44307-44308 , denotes ,
T22403 44309-44312 CD denotes 1.5
T22404 44313-44315 NN denotes μg
T22405 44316-44322 NN denotes rmIL-4
T22406 44322-44323 , denotes ,
T22407 44324-44326 CD denotes 50
T22408 44327-44329 NN denotes μg
T22409 44330-44339 JJ denotes anti-IL-4
T22410 44340-44343 NNP denotes mAb
T22411 44344-44345 -LRB- denotes (
T22412 44345-44350 NNP denotes 11B11
T22413 44351-44353 CC denotes or
T22414 44354-44360 NNP denotes MAB404
T22415 44360-44361 -RRB- denotes )
T22416 44361-44362 , denotes ,
T22417 44363-44365 CC denotes or
T22418 44366-44367 DT denotes a
T22419 44368-44375 NN denotes mixture
T22420 44376-44378 IN denotes of
T22421 44379-44382 CD denotes 1.5
T22422 44383-44385 NN denotes μg
T22423 44386-44392 NN denotes rmIL-4
T22424 44393-44397 CC denotes plus
T22425 44398-44400 CD denotes 50
T22426 44401-44403 NN denotes μg
T22427 44404-44413 JJ denotes anti-IL-4
T22428 44414-44417 NNP denotes mAb
T22429 44418-44419 -LRB- denotes (
T22430 44419-44424 NNP denotes 11B11
T22431 44425-44427 CC denotes or
T22432 44428-44434 NNP denotes MAB404
T22433 44434-44435 -RRB- denotes )
T22434 44436-44439 IN denotes for
T22435 44440-44441 CD denotes 7
T22436 44442-44444 NN denotes d.
T22437 44445-44455 RB denotes Thereafter
T22438 44455-44456 , denotes ,
T22439 44457-44463 JJ denotes spleen
T22440 44464-44467 CC denotes and
T22441 44468-44473 JJ denotes lymph
T22442 44474-44478 NN denotes node
T22443 44479-44484 NNS denotes cells
T22444 44485-44489 VBD denotes were
T22445 44490-44498 VBN denotes analyzed
T22446 44499-44501 IN denotes by
T22447 44502-44506 NN denotes flow
T22448 44507-44516 NN denotes cytometry
T22449 44517-44520 IN denotes for
T22450 44521-44524 NNP denotes CD3
T22451 44524-44525 , denotes ,
T22452 44526-44529 NNP denotes CD4
T22453 44529-44530 , denotes ,
T22454 44531-44534 CC denotes and
T22455 44535-44539 NNP denotes CD25
T22456 44540-44550 NN denotes expression
T22457 44550-44551 . denotes .
T22458 44552-44555 DT denotes The
T22459 44556-44566 JJ denotes anti-mouse
T22460 44567-44571 NN denotes IL-4
T22461 44572-44575 NNP denotes mAb
T22462 44576-44582 NNP denotes MAB404
T22463 44583-44586 VBD denotes was
T22464 44587-44595 VBN denotes obtained
T22465 44596-44600 IN denotes from
T22466 44601-44604 NNP denotes R&D
T22467 44605-44612 NNPS denotes Systems
T22468 44612-44613 , denotes ,
T22469 44614-44617 DT denotes the
T22470 44618-44624 JJ denotes second
T22471 44625-44635 JJ denotes anti-mouse
T22472 44636-44640 NN denotes IL-4
T22473 44641-44644 NNP denotes mAb
T22474 44645-44650 NNP denotes 11B11
T22475 44651-44654 VBD denotes was
T22476 44655-44664 VBN denotes purchased
T22477 44665-44669 IN denotes from
T22478 44670-44681 NNP denotes eBioscience
T22479 44681-44682 . denotes .
T22754 44684-44688 NN denotes ChIP
T22755 44688-44689 . denotes .
T22756 44690-44694 NN denotes ChIP
T22757 44695-44703 NN denotes analysis
T22758 44704-44707 VBD denotes was
T22759 44708-44717 VBN denotes performed
T22760 44718-44727 VBG denotes according
T22761 44728-44730 TO denotes to
T22762 44731-44734 DT denotes the
T22763 44735-44747 NN denotes manufacturer
T22764 44747-44749 POS denotes 's
T22765 44750-44758 NN denotes protocol
T22766 44759-44760 -LRB- denotes (
T22767 44760-44767 NNP denotes Upstate
T22768 44768-44781 NNP denotes Biotechnology
T22769 44781-44782 -RRB- denotes )
T22770 44783-44787 IN denotes with
T22771 44788-44791 DT denotes the
T22772 44792-44801 VBG denotes following
T22773 44802-44815 NNS denotes modifications
T22774 44815-44816 . denotes .
T22775 44817-44822 NNP denotes iTreg
T22776 44823-44826 CC denotes and
T22777 44827-44830 NNP denotes Th2
T22778 44831-44836 NNS denotes cells
T22779 44837-44841 VBD denotes were
T22780 44842-44847 VBN denotes fixed
T22781 44848-44852 IN denotes with
T22782 44853-44854 CD denotes 1
T22783 44854-44855 NN denotes %
T22784 44856-44868 NN denotes formaldehyde
T22785 44869-44872 IN denotes for
T22786 44873-44875 CD denotes 10
T22787 44876-44879 NN denotes min
T22788 44880-44882 IN denotes at
T22789 44883-44887 NN denotes room
T22790 44888-44899 NN denotes temperature
T22791 44899-44900 . denotes .
T22792 44901-44904 DT denotes The
T22793 44905-44914 NN denotes chromatin
T22794 44915-44918 VBD denotes was
T22795 44919-44926 VBN denotes sheared
T22796 44927-44929 TO denotes to
T22797 44930-44938 CD denotes 200-1000
T22798 44939-44941 NN denotes bp
T22799 44942-44944 IN denotes of
T22800 44945-44951 NN denotes length
T22801 44952-44954 IN denotes by
T22802 44955-44965 NN denotes sonication
T22803 44966-44970 IN denotes with
T22804 44971-44975 CD denotes five
T22805 44976-44982 NNS denotes pulses
T22806 44983-44985 IN denotes of
T22807 44986-44988 CD denotes 10
T22808 44989-44990 PRP denotes s
T22809 44991-44993 IN denotes at
T22810 44994-44996 CD denotes 30
T22811 44996-44997 NN denotes %
T22812 44998-45003 NN denotes power
T22813 45004-45005 -LRB- denotes (
T22814 45005-45013 NNP denotes Bandelin
T22815 45013-45014 -RRB- denotes )
T22816 45014-45015 . denotes .
T22817 45016-45019 DT denotes The
T22818 45020-45029 NN denotes chromatin
T22819 45030-45033 VBD denotes was
T22820 45034-45045 JJ denotes pre-cleared
T22821 45046-45049 IN denotes for
T22822 45050-45051 CD denotes 2
T22823 45052-45053 NN denotes h
T22824 45054-45058 IN denotes with
T22825 45059-45065 JJ denotes normal
T22826 45066-45071 NN denotes mouse
T22827 45072-45075 NNP denotes IgG
T22828 45076-45081 NNS denotes beads
T22829 45082-45085 CC denotes and
T22830 45086-45090 RB denotes then
T22831 45091-45100 VBN denotes incubated
T22832 45101-45105 IN denotes with
T22833 45106-45124 JJ denotes anti-GATA3-agarose
T22834 45125-45130 NNS denotes beads
T22835 45131-45132 -LRB- denotes (
T22836 45132-45135 CD denotes HG3
T22837 45136-45138 CD denotes 31
T22838 45138-45139 : denotes ;
T22839 45140-45145 NNP denotes Santa
T22840 45146-45150 NNP denotes Cruz
T22841 45151-45164 NNP denotes Biotechnology
T22842 45164-45165 -RRB- denotes )
T22843 45166-45169 IN denotes for
T22844 45170-45171 CD denotes 2
T22845 45172-45174 NN denotes h.
T22846 45175-45182 VBG denotes Washing
T22847 45183-45186 CC denotes and
T22848 45187-45194 NN denotes elution
T22849 45195-45202 NNS denotes buffers
T22850 45203-45207 VBD denotes were
T22851 45208-45212 VBN denotes used
T22852 45213-45222 VBG denotes according
T22853 45223-45225 TO denotes to
T22854 45226-45229 DT denotes the
T22855 45230-45238 NN denotes protocol
T22856 45239-45241 IN denotes of
T22857 45242-45249 NNP denotes Upstate
T22858 45250-45263 NNP denotes Biotechnology
T22859 45263-45264 . denotes .
T22860 45265-45275 NNS denotes Crosslinks
T22861 45276-45280 VBD denotes were
T22862 45281-45289 VBN denotes reversed
T22863 45290-45292 IN denotes by
T22864 45293-45303 NN denotes incubation
T22865 45304-45306 IN denotes at
T22866 45307-45309 CD denotes 65
T22867 45310-45311 CD denotes °
T22868 45311-45312 NNP denotes C
T22869 45313-45316 IN denotes for
T22870 45317-45318 CD denotes 4
T22871 45319-45320 NN denotes h
T22872 45321-45323 IN denotes in
T22873 45324-45327 DT denotes the
T22874 45328-45336 NN denotes presence
T22875 45337-45339 IN denotes of
T22876 45340-45343 CD denotes 0.2
T22877 45344-45345 NNP denotes M
T22878 45346-45350 NNP denotes NaCl
T22879 45350-45351 , denotes ,
T22880 45352-45355 CC denotes and
T22881 45356-45359 DT denotes the
T22882 45360-45363 NN denotes DNA
T22883 45364-45367 VBD denotes was
T22884 45368-45376 VBN denotes purified
T22885 45377-45379 IN denotes by
T22886 45380-45397 NN denotes phenol/chloroform
T22887 45398-45408 NN denotes extraction
T22888 45408-45409 . denotes .
T22889 45410-45413 DT denotes The
T22890 45414-45420 NN denotes amount
T22891 45421-45423 IN denotes of
T22892 45424-45427 NNP denotes DNA
T22893 45428-45431 VBD denotes was
T22894 45432-45442 VBN denotes determined
T22895 45443-45445 IN denotes by
T22896 45446-45458 JJ denotes conventional
T22897 45459-45462 NNP denotes PCR
T22898 45462-45463 . denotes .
T22899 45464-45467 DT denotes The
T22900 45468-45471 NNP denotes PCR
T22901 45472-45481 VBD denotes addressed
T22902 45482-45485 IN denotes for
T22903 45486-45489 DT denotes the
T22904 45490-45495 CD denotes FOXP3
T22905 45496-45504 NN denotes promoter
T22906 45505-45511 NN denotes region
T22907 45512-45513 NN denotes −
T22908 45513-45516 CD denotes 246
T22909 45517-45519 TO denotes to
T22910 45520-45521 CD denotes −
T22911 45521-45524 CD denotes 511
T22912 45525-45528 CC denotes and
T22913 45529-45532 VBD denotes was
T22914 45533-45542 VBN denotes performed
T22915 45543-45548 VBG denotes using
T22916 45549-45552 DT denotes the
T22917 45553-45562 VBG denotes following
T22918 45563-45570 NNS denotes primers
T22919 45570-45571 : denotes :
T22920 45572-45573 CD denotes 5
T22921 45573-45574 SYM denotes ′
T22922 45574-45575 : denotes -
T22923 45575-45590 NN denotes gtgccctttacgagt
T22924 45591-45599 JJ denotes catctg-3
T22925 45599-45600 NN denotes ′
T22926 45601-45604 CC denotes and
T22927 45605-45606 CD denotes 5
T22928 45606-45607 SYM denotes ′
T22929 45607-45608 : denotes -
T22930 45608-45631 JJ denotes gtgccctttacgagtcatctg-3
T22931 45631-45632 NN denotes ′
T22932 45632-45633 . denotes .
T22933 45634-45637 DT denotes The
T22934 45638-45641 NNP denotes PCR
T22935 45642-45650 NNS denotes products
T22936 45651-45655 VBD denotes were
T22937 45656-45666 VBN denotes visualized
T22938 45667-45672 VBG denotes using
T22939 45673-45675 DT denotes an
T22940 45676-45684 NN denotes ethidium
T22941 45685-45692 NN denotes bromide
T22942 45693-45696 NN denotes gel
T22943 45696-45697 . denotes .
T23192 45699-45708 JJ denotes Pull-down
T23193 45709-45714 NN denotes assay
T23194 45714-45715 . denotes .
T23195 45716-45719 CD denotes CD4
T23196 45719-45720 CD denotes +
T23197 45721-45722 NNP denotes T
T23198 45723-45728 NNS denotes cells
T23199 45729-45733 VBD denotes were
T23200 45734-45744 VBN denotes stimulated
T23201 45745-45749 IN denotes with
T23202 45750-45753 NNP denotes PMA
T23203 45754-45757 CC denotes and
T23204 45758-45767 NN denotes ionomycin
T23205 45768-45771 IN denotes for
T23206 45772-45773 CD denotes 2
T23207 45774-45775 NN denotes h
T23208 45776-45778 IN denotes at
T23209 45779-45781 CD denotes 37
T23210 45781-45782 CD denotes °
T23211 45782-45783 NNP denotes C
T23212 45783-45784 . denotes .
T23213 45785-45788 DT denotes The
T23214 45789-45794 NNS denotes cells
T23215 45795-45799 VBD denotes were
T23216 45800-45808 VBN denotes pelleted
T23217 45808-45809 , denotes ,
T23218 45810-45821 VBN denotes resuspended
T23219 45822-45824 IN denotes in
T23220 45825-45831 NN denotes buffer
T23221 45832-45833 NNP denotes C
T23222 45834-45835 -LRB- denotes (
T23223 45835-45837 CD denotes 20
T23224 45838-45840 NNP denotes mM
T23225 45841-45846 NNP denotes HEPES
T23226 45847-45848 NNP denotes [
T23227 45848-45850 NNP denotes pH
T23228 45851-45854 CD denotes 7.9
T23229 45854-45855 NNP denotes ]
T23230 45855-45856 , denotes ,
T23231 45857-45860 CD denotes 420
T23232 45861-45863 NNP denotes mM
T23233 45864-45868 NNP denotes NaCl
T23234 45868-45869 , denotes ,
T23235 45870-45873 CD denotes 1.5
T23236 45874-45876 NNP denotes mM
T23237 45877-45882 NNP denotes MgCl2
T23238 45882-45883 , denotes ,
T23239 45884-45887 CD denotes 0.2
T23240 45888-45890 NNP denotes mM
T23241 45891-45895 NNP denotes EDTA
T23242 45895-45896 , denotes ,
T23243 45897-45898 CD denotes 1
T23244 45899-45901 NNP denotes mM
T23245 45902-45905 NNP denotes DTT
T23246 45905-45906 , denotes ,
T23247 45907-45915 NN denotes protease
T23248 45916-45926 NNS denotes inhibitors
T23249 45927-45928 NNP denotes [
T23250 45928-45933 NNP denotes Sigma
T23251 45933-45934 NNP denotes ]
T23252 45934-45935 . denotes .
T23253 45936-45939 CC denotes and
T23254 45940-45943 CD denotes 0.1
T23255 45943-45944 NN denotes %
T23256 45945-45950 NN denotes NP-40
T23257 45950-45951 -RRB- denotes )
T23258 45952-45955 CC denotes and
T23259 45956-45961 VBN denotes lysed
T23260 45962-45964 IN denotes on
T23261 45965-45968 NN denotes ice
T23262 45969-45972 IN denotes for
T23263 45973-45975 CD denotes 15
T23264 45976-45979 NN denotes min
T23265 45979-45980 . denotes .
T23266 45981-45990 NNP denotes Insoluble
T23267 45991-45999 NN denotes material
T23268 46000-46003 VBD denotes was
T23269 46004-46011 VBN denotes removed
T23270 46012-46014 IN denotes by
T23271 46015-46029 NN denotes centrifugation
T23272 46029-46030 . denotes .
T23273 46031-46034 DT denotes The
T23274 46035-46046 NN denotes supernatant
T23275 46047-46050 VBD denotes was
T23276 46051-46058 VBN denotes diluted
T23277 46059-46062 CD denotes 1:3
T23278 46063-46067 IN denotes with
T23279 46068-46074 NN denotes buffer
T23280 46075-46076 NNP denotes D
T23281 46077-46078 -LRB- denotes (
T23282 46078-46080 IN denotes as
T23283 46081-46087 NN denotes buffer
T23284 46088-46089 NNP denotes C
T23285 46089-46090 , denotes ,
T23286 46091-46094 CC denotes but
T23287 46095-46102 IN denotes without
T23288 46103-46107 NNP denotes NaCl
T23289 46107-46108 -RRB- denotes )
T23290 46108-46109 . denotes .
T23291 46110-46113 DT denotes The
T23292 46114-46121 NNS denotes lysates
T23293 46122-46126 VBD denotes were
T23294 46127-46136 VBN denotes incubated
T23295 46137-46141 IN denotes with
T23296 46142-46144 CD denotes 10
T23297 46145-46147 NN denotes μg
T23298 46148-46150 IN denotes of
T23299 46151-46155 NN denotes poly
T23300 46155-46156 -LRB- denotes (
T23301 46156-46161 JJ denotes dI-dC
T23302 46161-46162 -RRB- denotes )
T23303 46163-46164 -LRB- denotes (
T23304 46164-46169 NNP denotes Sigma
T23305 46169-46170 -RRB- denotes )
T23306 46171-46174 CC denotes and
T23307 46175-46177 CD denotes 70
T23308 46178-46180 NN denotes μl
T23309 46181-46183 IN denotes of
T23310 46184-46204 JJ denotes streptavidin-agarose
T23311 46205-46206 -LRB- denotes (
T23312 46206-46214 NNP denotes Amersham
T23313 46215-46226 NNPS denotes Biosciences
T23314 46226-46227 -RRB- denotes )
T23315 46228-46236 VBG denotes carrying
T23316 46237-46249 JJ denotes biotinylated
T23317 46250-46266 NNS denotes oligonucleotides
T23318 46266-46267 , denotes ,
T23319 46268-46271 IN denotes for
T23320 46272-46273 CD denotes 3
T23321 46274-46275 NN denotes h
T23322 46276-46278 IN denotes at
T23323 46279-46280 CD denotes 4
T23324 46281-46282 CD denotes °
T23325 46282-46283 NNP denotes C
T23326 46283-46284 . denotes .
T23327 46285-46288 DT denotes The
T23328 46289-46294 NNS denotes beads
T23329 46295-46299 VBD denotes were
T23330 46300-46306 VBN denotes washed
T23331 46307-46312 RB denotes twice
T23332 46313-46317 IN denotes with
T23333 46318-46324 NN denotes buffer
T23334 46325-46326 NNP denotes C
T23335 46326-46328 NNP denotes :D
T23336 46329-46330 -LRB- denotes (
T23337 46330-46333 CD denotes 1:3
T23338 46333-46334 -RRB- denotes )
T23339 46335-46338 CC denotes and
T23340 46339-46350 VBD denotes resuspended
T23341 46351-46353 IN denotes in
T23342 46354-46368 JJ denotes DTT-containing
T23343 46369-46376 VBG denotes loading
T23344 46377-46383 NN denotes buffer
T23345 46384-46385 -LRB- denotes (
T23346 46385-46391 NNP denotes NuPAGE
T23347 46391-46392 : denotes ;
T23348 46393-46403 NNP denotes Invitrogen
T23349 46403-46404 -RRB- denotes )
T23350 46404-46405 , denotes ,
T23351 46406-46412 VBN denotes heated
T23352 46413-46415 TO denotes to
T23353 46416-46418 CD denotes 70
T23354 46419-46420 CD denotes °
T23355 46420-46421 NNP denotes C
T23356 46422-46425 IN denotes for
T23357 46426-46428 CD denotes 10
T23358 46429-46432 NN denotes min
T23359 46432-46433 , denotes ,
T23360 46434-46437 CC denotes and
T23361 46438-46441 DT denotes the
T23362 46442-46449 NNS denotes eluants
T23363 46450-46452 IN denotes on
T23364 46453-46454 DT denotes a
T23365 46455-46461 NNP denotes NuPAGE
T23366 46462-46463 CD denotes 4
T23367 46464-46466 CD denotes 12
T23368 46466-46467 NN denotes %
T23369 46468-46476 JJ denotes bis-tris
T23370 46477-46480 NN denotes gel
T23371 46481-46482 -LRB- denotes (
T23372 46482-46492 NNP denotes Invitrogen
T23373 46492-46493 -RRB- denotes )
T23374 46493-46494 . denotes .
T23375 46495-46498 DT denotes The
T23376 46499-46507 NNS denotes proteins
T23377 46508-46512 VBD denotes were
T23378 46513-46527 VBN denotes electroblotted
T23379 46528-46532 IN denotes onto
T23380 46533-46534 DT denotes a
T23381 46535-46539 NNP denotes PVDF
T23382 46540-46548 NN denotes membrane
T23383 46549-46550 -LRB- denotes (
T23384 46550-46558 NNP denotes Amersham
T23385 46559-46570 NNPS denotes Biosciences
T23386 46570-46571 -RRB- denotes )
T23387 46572-46575 CC denotes and
T23388 46576-46584 VBD denotes detected
T23389 46585-46590 VBG denotes using
T23390 46591-46593 DT denotes an
T23391 46594-46604 JJ denotes anti-GATA3
T23392 46605-46608 NNP denotes mAb
T23393 46609-46610 -LRB- denotes (
T23394 46610-46615 NNP denotes Santa
T23395 46616-46620 NNP denotes Cruz
T23396 46621-46634 NNP denotes Biotechnology
T23397 46634-46635 -RRB- denotes )
T23398 46635-46636 . denotes .
T23399 46637-46648 VBN denotes Accumulated
T23400 46649-46656 NNS denotes signals
T23401 46657-46661 VBD denotes were
T23402 46662-46670 VBN denotes analyzed
T23403 46671-46676 VBG denotes using
T23404 46677-46681 NNP denotes AIDA
T23405 46682-46690 NN denotes software
T23406 46691-46692 -LRB- denotes (
T23407 46692-46699 JJS denotes Raytest
T23408 46699-46700 -RRB- denotes )
T23409 46700-46701 . denotes .
T23591 7387-7390 CD denotes Th2
T23592 7391-7396 NNP denotes Cells
T23593 7397-7400 MD denotes Can
T23594 7400-7403 RB denotes not
T23595 7404-7410 VB denotes Induce
T23596 7411-7416 NNP denotes FOXP3
T23597 7417-7427 NNP denotes Expression
T23598 7428-7429 -LRB- denotes (
T23599 7429-7430 NNP denotes A
T23600 7430-7431 -RRB- denotes )
T23601 7432-7437 NNP denotes Human
T23602 7438-7439 NNP denotes T
T23603 7440-7445 NNS denotes cells
T23604 7446-7450 VBD denotes were
T23605 7451-7460 VBN denotes activated
T23606 7461-7465 IN denotes with
T23607 7466-7477 JJ denotes plate-bound
T23608 7478-7486 JJ denotes anti-CD3
T23609 7486-7487 NN denotes /
T23610 7487-7491 CD denotes CD28
T23611 7492-7496 IN denotes with
T23612 7497-7499 CC denotes or
T23613 7500-7507 IN denotes without
T23614 7508-7513 NNP denotes TGF-β
T23615 7514-7516 IN denotes as
T23616 7517-7526 VBN denotes indicated
T23617 7527-7529 IN denotes on
T23618 7530-7533 DT denotes the
T23619 7534-7540 NNS denotes x-axis
T23620 7541-7543 IN denotes of
T23621 7544-7545 -LRB- denotes (
T23622 7545-7546 NNP denotes B
T23623 7546-7547 -RRB- denotes )
T23624 7547-7548 . denotes .
T23625 7549-7554 NNS denotes Cells
T23626 7555-7559 VBD denotes were
T23627 7560-7569 VBN denotes harvested
T23628 7570-7575 IN denotes after
T23629 7576-7577 CD denotes 5
T23630 7578-7582 NNS denotes days
T23631 7583-7586 CC denotes and
T23632 7587-7592 CD denotes FOXP3
T23633 7593-7597 NNP denotes mRNA
T23634 7598-7601 VBD denotes was
T23635 7602-7612 VBN denotes quantified
T23636 7613-7615 IN denotes by
T23637 7616-7625 JJ denotes real-time
T23638 7626-7629 NNP denotes PCR
T23639 7629-7630 . denotes .
T23640 7631-7635 NNS denotes Bars
T23641 7636-7640 VBP denotes show
T23642 7641-7644 DT denotes the
T23643 7645-7649 JJ denotes mean
T23644 7650-7651 NN denotes ±
T23645 7652-7654 NNP denotes SD
T23646 7655-7657 IN denotes of
T23647 7658-7659 CD denotes 4
T23648 7660-7671 JJ denotes independent
T23649 7672-7683 NNS denotes experiments
T23650 7683-7684 . denotes .
T23651 7685-7686 -LRB- denotes (
T23652 7686-7687 NNP denotes B
T23653 7687-7688 -RRB- denotes )
T23654 7689-7691 IN denotes In
T23655 7692-7697 NN denotes vitro
T23656 7698-7712 VBN denotes differentiated
T23657 7713-7716 NNP denotes Th1
T23658 7716-7717 , denotes ,
T23659 7718-7721 NNP denotes Th2
T23660 7721-7722 , denotes ,
T23661 7723-7725 CC denotes or
T23662 7726-7731 NNP denotes iTreg
T23663 7732-7737 NNS denotes cells
T23664 7738-7742 VBD denotes were
T23665 7743-7752 VBN denotes activated
T23666 7753-7757 IN denotes with
T23667 7758-7766 JJ denotes anti-CD3
T23668 7766-7767 NN denotes /
T23669 7767-7771 NNP denotes CD28
T23670 7771-7772 , denotes ,
T23671 7773-7778 NNP denotes TGF-β
T23672 7778-7779 , denotes ,
T23673 7780-7782 CC denotes or
T23674 7783-7792 JJ denotes anti-IL-4
T23675 7793-7795 IN denotes as
T23676 7796-7805 VBN denotes indicated
T23677 7805-7806 . denotes .
T23678 7807-7810 DT denotes The
T23679 7811-7820 NN denotes phenotype
T23680 7821-7823 IN denotes of
T23681 7824-7829 DT denotes these
T23682 7830-7835 NNS denotes cells
T23683 7836-7839 VBD denotes was
T23684 7840-7849 VBN denotes confirmed
T23685 7850-7852 IN denotes by
T23686 7853-7857 NNP denotes FACS
T23687 7858-7861 CC denotes and
T23688 7862-7875 NN denotes proliferation
T23689 7876-7884 NN denotes analysis
T23690 7885-7886 -LRB- denotes (
T23691 7886-7892 NN denotes Figure
T23692 7893-7895 CD denotes S1
T23693 7895-7896 -RRB- denotes )
T23694 7896-7897 . denotes .
T23695 7898-7902 NNS denotes Bars
T23696 7903-7907 VBP denotes show
T23697 7908-7911 DT denotes the
T23698 7912-7916 JJ denotes mean
T23699 7917-7918 NN denotes ±
T23700 7919-7921 NNP denotes SD
T23701 7922-7924 IN denotes of
T23702 7925-7929 CD denotes four
T23703 7930-7941 JJ denotes independent
T23704 7942-7953 NNS denotes experiments
T23705 7953-7954 . denotes .
T23907 10140-10143 CD denotes Th2
T23908 10144-10149 NNS denotes Cells
T23909 10150-10152 VBP denotes Do
T23910 10153-10156 RB denotes Not
T23911 10157-10164 NNP denotes Express
T23912 10165-10170 NNP denotes FOXP3
T23913 10171-10172 -LRB- denotes (
T23914 10172-10173 NNP denotes A
T23915 10173-10174 -RRB- denotes )
T23916 10175-10188 NNP denotes Intracellular
T23917 10189-10193 NNP denotes FACS
T23918 10194-10202 NN denotes analysis
T23919 10203-10205 IN denotes of
T23920 10206-10211 CD denotes FOXP3
T23921 10212-10222 NN denotes expression
T23922 10223-10225 IN denotes in
T23923 10226-10229 NNP denotes Th1
T23924 10229-10230 , denotes ,
T23925 10231-10234 NNP denotes Th2
T23926 10234-10235 , denotes ,
T23927 10236-10238 CC denotes or
T23928 10239-10259 JJ denotes iTreg-differentiated
T23929 10260-10265 NNS denotes cells
T23930 10266-10267 -LRB- denotes (
T23931 10267-10270 CD denotes two
T23932 10271-10277 NNS denotes rounds
T23933 10277-10278 , denotes ,
T23934 10279-10288 NN denotes phenotype
T23935 10289-10292 VBP denotes see
T23936 10293-10299 NN denotes Figure
T23937 10300-10302 CD denotes S1
T23938 10302-10303 -RRB- denotes )
T23939 10303-10304 , denotes ,
T23940 10305-10311 VBD denotes rested
T23941 10312-10314 CC denotes or
T23942 10315-10324 VBD denotes activated
T23943 10324-10325 , denotes ,
T23944 10326-10330 IN denotes with
T23945 10331-10333 CC denotes or
T23946 10334-10341 IN denotes without
T23947 10342-10347 NNP denotes TGF-β
T23948 10347-10348 . denotes .
T23949 10349-10354 CD denotes FOXP3
T23950 10355-10365 NN denotes expression
T23951 10366-10369 VBD denotes was
T23952 10370-10378 VBN denotes measured
T23953 10379-10384 IN denotes after
T23954 10385-10386 CD denotes 5
T23955 10387-10388 LS denotes d
T23956 10389-10391 IN denotes in
T23957 10392-10399 NN denotes culture
T23958 10399-10400 . denotes .
T23959 10401-10404 DT denotes The
T23960 10405-10408 VBP denotes dot
T23961 10409-10414 NNS denotes blots
T23962 10415-10418 VBP denotes are
T23963 10419-10433 NN denotes representative
T23964 10434-10436 IN denotes of
T23965 10437-10442 CD denotes three
T23966 10443-10454 JJ denotes independent
T23967 10455-10466 NNS denotes experiments
T23968 10466-10467 . denotes .
T23969 10468-10469 -LRB- denotes (
T23970 10469-10470 NNP denotes B
T23971 10470-10471 -RRB- denotes )
T23972 10472-10477 VBZ denotes Shows
T23973 10478-10481 DT denotes the
T23974 10482-10486 JJ denotes same
T23975 10487-10499 JJ denotes experimental
T23976 10500-10505 NN denotes setup
T23977 10505-10506 , denotes ,
T23978 10507-10510 CC denotes but
T23979 10511-10520 RB denotes naturally
T23980 10521-10530 VBG denotes occurring
T23981 10531-10534 JJ denotes Th2
T23982 10535-10540 NNS denotes cells
T23983 10541-10545 VBD denotes were
T23984 10546-10554 VBN denotes analyzed
T23985 10554-10555 . denotes .
T23986 10556-10560 NNS denotes Data
T23987 10561-10564 VBP denotes are
T23988 10565-10579 NN denotes representative
T23989 10580-10582 IN denotes of
T23990 10583-10588 CD denotes three
T23991 10589-10600 JJ denotes independent
T23992 10601-10612 NNS denotes experiments
T23993 10612-10613 . denotes .
T24303 10624-10627 SYM denotes Th2
T24304 10627-10628 : denotes -
T24305 10629-10631 CC denotes or
T24306 10632-10636 NN denotes IL-4
T24307 10637-10646 NNP denotes Producing
T24308 10647-10652 NNP denotes Cells
T24309 10653-10657 NNP denotes Lack
T24310 10658-10663 NNP denotes FOXP3
T24311 10664-10665 -LRB- denotes (
T24312 10665-10666 NNP denotes A
T24313 10666-10667 -RRB- denotes )
T24314 10668-10672 NNP denotes FACS
T24315 10673-10681 NN denotes analysis
T24316 10682-10684 IN denotes of
T24317 10685-10698 JJ denotes intracellular
T24318 10699-10704 CD denotes FOXP3
T24319 10705-10708 CC denotes and
T24320 10709-10713 NN denotes IL-4
T24321 10714-10724 NN denotes expression
T24322 10725-10734 VBG denotes following
T24323 10735-10748 NNP denotes PMA/Ionomycin
T24324 10749-10760 NN denotes stimulation
T24325 10760-10761 . denotes .
T24326 10762-10765 CD denotes CD4
T24327 10765-10766 CD denotes +
T24328 10767-10768 NNP denotes T
T24329 10769-10774 NNS denotes cells
T24330 10775-10779 VBD denotes were
T24331 10780-10785 VBN denotes gated
T24332 10786-10788 IN denotes on
T24333 10789-10792 DT denotes the
T24334 10793-10798 NN denotes basis
T24335 10799-10801 IN denotes of
T24336 10802-10808 CD denotes CD45RO
T24337 10809-10812 CC denotes and
T24338 10813-10817 CD denotes CD25
T24339 10818-10825 NN denotes surface
T24340 10826-10836 NN denotes expression
T24341 10837-10838 -LRB- denotes (
T24342 10838-10843 JJ denotes upper
T24343 10844-10849 NN denotes panel
T24344 10849-10850 -RRB- denotes )
T24345 10850-10851 , denotes ,
T24346 10852-10855 CC denotes and
T24347 10856-10861 VBD denotes gated
T24348 10862-10867 NNS denotes cells
T24349 10868-10871 VBP denotes are
T24350 10872-10877 VBN denotes shown
T24351 10878-10883 IN denotes below
T24352 10884-10887 IN denotes for
T24353 10888-10891 DT denotes the
T24354 10892-10898 NNP denotes CD45RO
T24355 10898-10899 NNP denotes +
T24356 10899-10903 NNP denotes CD25
T24357 10903-10904 NNP denotes −
T24358 10905-10906 -LRB- denotes (
T24359 10906-10907 NNP denotes A
T24360 10907-10908 , denotes ,
T24361 10909-10913 VBD denotes left
T24362 10914-10919 NN denotes panel
T24363 10919-10920 -RRB- denotes )
T24364 10920-10921 , denotes ,
T24365 10922-10925 DT denotes the
T24366 10926-10932 NNP denotes CD45RO
T24367 10932-10933 NNP denotes +
T24368 10933-10937 NNP denotes CD25
T24369 10937-10938 NNP denotes +
T24370 10939-10940 -LRB- denotes (
T24371 10940-10945 JJ denotes right
T24372 10946-10951 NN denotes panel
T24373 10951-10952 -RRB- denotes )
T24374 10952-10953 , denotes ,
T24375 10954-10957 CC denotes and
T24376 10958-10961 DT denotes the
T24377 10962-10968 NNP denotes CD45RO
T24378 10968-10969 NNP denotes −
T24379 10969-10973 NNP denotes CD25
T24380 10973-10974 VBD denotes −
T24381 10975-10982 NNS denotes subsets
T24382 10983-10984 -LRB- denotes (
T24383 10984-10991 JJ denotes central
T24384 10992-10997 NN denotes panel
T24385 10997-10998 -RRB- denotes )
T24386 10998-10999 . denotes .
T24387 11000-11001 DT denotes A
T24388 11002-11013 JJ denotes statistical
T24389 11014-11022 NN denotes analysis
T24390 11023-11025 IN denotes of
T24391 11026-11031 CD denotes eight
T24392 11032-11043 JJ denotes independent
T24393 11044-11050 NNS denotes donors
T24394 11051-11056 IN denotes after
T24395 11057-11068 NN denotes subtraction
T24396 11069-11071 IN denotes of
T24397 11072-11075 DT denotes the
T24398 11076-11083 NN denotes isotype
T24399 11084-11091 NN denotes control
T24400 11092-11095 VBP denotes are
T24401 11096-11101 VBN denotes shown
T24402 11102-11104 IN denotes in
T24403 11105-11106 -LRB- denotes (
T24404 11106-11107 NNP denotes B
T24405 11107-11108 -RRB- denotes )
T24406 11108-11109 . denotes .
T24407 11110-11113 DT denotes The
T24408 11114-11120 JJ denotes dotted
T24409 11121-11125 JJ denotes gray
T24410 11126-11130 NN denotes line
T24411 11131-11140 VBZ denotes indicates
T24412 11141-11144 DT denotes the
T24413 11145-11147 NNP denotes IC
T24414 11148-11158 NN denotes background
T24415 11159-11164 NN denotes level
T24416 11164-11165 . denotes .
T24417 11166-11169 DT denotes The
T24418 11170-11175 NN denotes error
T24419 11176-11180 NNS denotes bars
T24420 11181-11185 VBP denotes show
T24421 11186-11189 DT denotes the
T24422 11190-11195 NN denotes error
T24423 11196-11198 IN denotes of
T24424 11199-11202 DT denotes the
T24425 11203-11207 NN denotes mean
T24426 11207-11208 . denotes .
T24427 11209-11210 -LRB- denotes (
T24428 11210-11211 $ denotes C
T24429 11211-11212 -RRB- denotes )
T24430 11213-11222 RB denotes Similarly
T24431 11222-11223 , denotes ,
T24432 11224-11225 DT denotes a
T24433 11226-11229 JJ denotes Th2
T24434 11230-11235 NN denotes clone
T24435 11236-11237 -LRB- denotes (
T24436 11237-11240 CD denotes BR8
T24437 11240-11241 -RRB- denotes )
T24438 11241-11242 , denotes ,
T24439 11243-11248 NNP denotes CRTH2
T24440 11248-11249 NNP denotes +
T24441 11250-11253 NNP denotes Th2
T24442 11254-11259 NNS denotes cells
T24443 11259-11260 , denotes ,
T24444 11261-11275 JJ denotes IL-4-secreting
T24445 11276-11281 NNS denotes cells
T24446 11281-11282 , denotes ,
T24447 11283-11286 CC denotes and
T24448 11287-11293 NN denotes memory
T24449 11294-11295 NNP denotes T
T24450 11296-11301 NNS denotes cells
T24451 11302-11303 -LRB- denotes (
T24452 11303-11309 CD denotes CD45RO
T24453 11309-11310 -RRB- denotes )
T24454 11311-11315 VBD denotes were
T24455 11316-11323 VBN denotes stained
T24456 11324-11327 IN denotes for
T24457 11328-11333 CD denotes FOXP3
T24458 11334-11337 CC denotes and
T24459 11338-11342 NN denotes IL-4
T24460 11342-11343 . denotes .
T24461 11344-11348 NNS denotes Data
T24462 11349-11352 VBP denotes are
T24463 11353-11367 NN denotes representative
T24464 11368-11370 IN denotes of
T24465 11371-11376 CD denotes three
T24466 11377-11388 JJ denotes independent
T24467 11389-11400 NNS denotes experiments
T24468 11400-11401 . denotes .
T24825 13213-13218 CD denotes FOXP3
T24826 13219-13228 NNP denotes Induction
T24827 13229-13235 IN denotes During
T24828 13236-13239 DT denotes the
T24829 13240-13255 NNP denotes Differentiation
T24830 13256-13263 NNP denotes Process
T24831 13264-13265 -LRB- denotes (
T24832 13265-13266 NNP denotes A
T24833 13266-13267 -RRB- denotes )
T24834 13268-13273 NNP denotes Human
T24835 13274-13277 NNP denotes CD4
T24836 13277-13278 NNP denotes +
T24837 13278-13284 NNP denotes CD45RA
T24838 13284-13285 VBD denotes +
T24839 13286-13287 NNP denotes T
T24840 13288-13293 NNS denotes cells
T24841 13294-13298 VBD denotes were
T24842 13299-13308 VBN denotes activated
T24843 13309-13313 IN denotes with
T24844 13314-13325 JJ denotes plate-bound
T24845 13326-13334 JJ denotes anti-CD3
T24846 13334-13335 NN denotes /
T24847 13335-13339 CD denotes CD28
T24848 13340-13342 IN denotes in
T24849 13343-13346 DT denotes the
T24850 13347-13355 NN denotes presence
T24851 13356-13358 IN denotes of
T24852 13359-13364 NNP denotes TGF-β
T24853 13365-13366 -LRB- denotes (
T24854 13366-13367 CD denotes 5
T24855 13368-13373 NN denotes ng/ml
T24856 13373-13374 -RRB- denotes )
T24857 13375-13377 CC denotes or
T24858 13378-13382 NN denotes IL-4
T24859 13383-13384 -LRB- denotes (
T24860 13384-13386 CD denotes 25
T24861 13387-13392 NN denotes ng/ml
T24862 13392-13393 -RRB- denotes )
T24863 13393-13394 . denotes .
T24864 13395-13398 DT denotes The
T24865 13399-13404 NNS denotes cells
T24866 13405-13409 VBD denotes were
T24867 13410-13419 VBN denotes harvested
T24868 13420-13422 IN denotes at
T24869 13423-13432 JJ denotes different
T24870 13433-13437 NN denotes time
T24871 13438-13444 NNS denotes points
T24872 13444-13445 , denotes ,
T24873 13446-13449 CC denotes and
T24874 13450-13454 NNP denotes mRNA
T24875 13455-13458 VBD denotes was
T24876 13459-13469 VBN denotes quantified
T24877 13470-13472 IN denotes by
T24878 13473-13482 JJ denotes real-time
T24879 13483-13486 NNP denotes PCR
T24880 13487-13490 IN denotes for
T24881 13491-13496 NNP denotes FOXP3
T24882 13497-13500 CC denotes and
T24883 13501-13506 NNP denotes GATA3
T24884 13507-13517 NN denotes expression
T24885 13517-13518 . denotes .
T24886 13519-13523 NNS denotes Bars
T24887 13524-13528 VBP denotes show
T24888 13529-13532 DT denotes the
T24889 13533-13537 JJ denotes mean
T24890 13538-13539 NN denotes ±
T24891 13540-13542 NNP denotes SD
T24892 13543-13545 IN denotes of
T24893 13546-13551 CD denotes three
T24894 13552-13563 JJ denotes independent
T24895 13564-13575 NNS denotes experiments
T24896 13575-13576 . denotes .
T24897 13578-13579 -LRB- denotes (
T24898 13579-13580 NNP denotes B
T24899 13580-13581 -RRB- denotes )
T24900 13582-13595 NNP denotes Intracellular
T24901 13596-13601 NNP denotes GATA3
T24902 13602-13605 CC denotes and
T24903 13606-13611 NNP denotes FOXP3
T24904 13612-13620 NN denotes staining
T24905 13621-13623 VBZ denotes is
T24906 13624-13629 VBN denotes shown
T24907 13630-13635 IN denotes after
T24908 13636-13644 NN denotes exposure
T24909 13645-13647 IN denotes of
T24910 13648-13651 NNP denotes CD4
T24911 13651-13652 NNP denotes +
T24912 13652-13658 NNP denotes CD45RA
T24913 13658-13659 VBD denotes +
T24914 13660-13661 NNP denotes T
T24915 13662-13667 NNS denotes cells
T24916 13668-13670 TO denotes to
T24917 13671-13686 JJ denotes differentiating
T24918 13687-13697 NNS denotes conditions
T24919 13698-13700 IN denotes as
T24920 13701-13703 IN denotes in
T24921 13704-13708 NN denotes part
T24922 13709-13710 DT denotes A
T24923 13711-13713 IN denotes of
T24924 13714-13717 DT denotes the
T24925 13718-13724 NN denotes figure
T24926 13724-13725 . denotes .
T24927 13726-13730 NNS denotes Data
T24928 13731-13734 VBP denotes are
T24929 13735-13749 NN denotes representative
T24930 13750-13752 IN denotes of
T24931 13753-13758 CD denotes three
T24932 13759-13770 JJ denotes independent
T24933 13771-13782 NNS denotes experiments
T24934 13782-13783 . denotes .
T25390 16498-16504 NN denotes Effect
T25391 16505-16507 IN denotes of
T25392 16508-16512 NN denotes IL-4
T25393 16513-16515 IN denotes on
T25394 16516-16521 NNP denotes FOXP3
T25395 16522-16531 NNP denotes Induction
T25396 16532-16533 -LRB- denotes (
T25397 16533-16534 NNP denotes A
T25398 16534-16535 -RRB- denotes )
T25399 16536-16537 DT denotes A
T25400 16538-16549 JJ denotes statistical
T25401 16550-16558 NN denotes analysis
T25402 16559-16562 VBD denotes was
T25403 16563-16572 VBN denotes performed
T25404 16573-16577 IN denotes with
T25405 16578-16581 CD denotes six
T25406 16582-16588 NNS denotes donors
T25407 16589-16591 IN denotes on
T25408 16592-16595 NN denotes day
T25409 16596-16597 CD denotes 5
T25410 16598-16599 -LRB- denotes (
T25411 16599-16604 NN denotes TGF-β
T25412 16605-16606 -LRB- denotes (
T25413 16606-16608 CD denotes 10
T25414 16609-16614 NN denotes ng/ml
T25415 16614-16615 -RRB- denotes )
T25416 16616-16619 CC denotes and
T25417 16620-16624 IN denotes with
T25418 16625-16627 CC denotes or
T25419 16628-16635 IN denotes without
T25420 16636-16640 NN denotes IL-4
T25421 16641-16642 -LRB- denotes (
T25422 16642-16645 CD denotes 100
T25423 16646-16651 NN denotes ng/ml
T25424 16651-16652 -RRB- denotes )
T25425 16652-16653 -RRB- denotes )
T25426 16653-16654 : denotes ;
T25427 16655-16660 VBN denotes Shown
T25428 16661-16663 VBZ denotes is
T25429 16664-16667 DT denotes the
T25430 16668-16672 NN denotes mean
T25431 16672-16673 , denotes ,
T25432 16674-16677 CC denotes and
T25433 16678-16683 NN denotes error
T25434 16684-16688 NNS denotes bars
T25435 16689-16698 VBD denotes indicated
T25436 16699-16702 DT denotes the
T25437 16703-16705 NNP denotes SD
T25438 16706-16708 IN denotes of
T25439 16709-16712 CD denotes six
T25440 16713-16719 NNS denotes donors
T25441 16719-16720 . denotes .
T25442 16721-16732 NNP denotes Statistical
T25443 16733-16741 NN denotes analysis
T25444 16742-16745 VBD denotes was
T25445 16746-16755 VBN denotes performed
T25446 16756-16761 VBG denotes using
T25447 16762-16765 DT denotes the
T25448 16766-16773 NNP denotes Dunnett
T25449 16774-16778 NN denotes test
T25450 16778-16779 . denotes .
T25451 16780-16791 NNP denotes Statistical
T25452 16792-16804 NN denotes significance
T25453 16805-16807 VBZ denotes is
T25454 16808-16817 VBN denotes indicated
T25455 16818-16820 IN denotes by
T25456 16821-16830 NNS denotes asterisks
T25457 16831-16832 -LRB- denotes (
T25458 16832-16833 SYM denotes *
T25459 16833-16834 FW denotes p
T25460 16835-16836 FW denotes ≤
T25461 16837-16841 CD denotes 0.05
T25462 16841-16842 , denotes ,
T25463 16843-16845 SYM denotes **
T25464 16845-16846 FW denotes p
T25465 16847-16848 FW denotes ≤
T25466 16849-16853 CD denotes 0.01
T25467 16853-16854 , denotes ,
T25468 16855-16862 NNP denotes Dunnett
T25469 16862-16863 -RRB- denotes )
T25470 16863-16864 . denotes .
T25471 16865-16866 -LRB- denotes (
T25472 16866-16867 NNP denotes B
T25473 16867-16868 -RRB- denotes )
T25474 16869-16872 NNP denotes CD4
T25475 16872-16873 NNP denotes +
T25476 16873-16879 NNP denotes CD45RA
T25477 16879-16880 VBD denotes +
T25478 16881-16886 NNS denotes cells
T25479 16887-16891 VBD denotes were
T25480 16892-16901 VBN denotes activated
T25481 16902-16904 IN denotes in
T25482 16905-16908 DT denotes the
T25483 16909-16917 NN denotes presence
T25484 16918-16920 IN denotes of
T25485 16921-16922 DT denotes a
T25486 16923-16931 JJ denotes constant
T25487 16932-16945 NN denotes concentration
T25488 16946-16948 IN denotes of
T25489 16949-16954 NNP denotes TGF-β
T25490 16955-16956 -LRB- denotes (
T25491 16956-16957 CD denotes 5
T25492 16958-16963 NN denotes ng/ml
T25493 16963-16964 -RRB- denotes )
T25494 16965-16969 IN denotes with
T25495 16970-16972 DT denotes an
T25496 16973-16983 VBG denotes increasing
T25497 16984-16997 NN denotes concentration
T25498 16998-17000 IN denotes of
T25499 17001-17005 NN denotes IL-4
T25500 17005-17006 , denotes ,
T25501 17007-17009 IN denotes as
T25502 17010-17019 VBN denotes indicated
T25503 17019-17020 . denotes .
T25504 17021-17026 NNS denotes Cells
T25505 17027-17031 VBD denotes were
T25506 17032-17041 VBN denotes harvested
T25507 17042-17045 IN denotes for
T25508 17046-17050 NNP denotes mRNA
T25509 17051-17065 NN denotes quantification
T25510 17066-17071 IN denotes after
T25511 17072-17073 CD denotes 5
T25512 17074-17076 NN denotes d.
T25513 17077-17078 -LRB- denotes (
T25514 17078-17079 NNP denotes C
T25515 17079-17080 -RRB- denotes )
T25516 17081-17084 NNP denotes CD4
T25517 17084-17085 NNP denotes +
T25518 17085-17091 NNP denotes CD45RA
T25519 17091-17092 VBD denotes +
T25520 17093-17098 NNS denotes cells
T25521 17099-17103 VBD denotes were
T25522 17104-17114 VBN denotes stimulated
T25523 17115-17117 IN denotes in
T25524 17118-17123 NN denotes vitro
T25525 17124-17128 IN denotes with
T25526 17129-17140 JJ denotes plate-bound
T25527 17141-17149 JJ denotes anti-CD3
T25528 17149-17150 NN denotes /
T25529 17150-17154 NNP denotes CD28
T25530 17154-17155 , denotes ,
T25531 17156-17161 NNP denotes TGF-β
T25532 17162-17163 -LRB- denotes (
T25533 17163-17170 CD denotes 10ng/ml
T25534 17170-17171 -RRB- denotes )
T25535 17171-17172 , denotes ,
T25536 17173-17176 CC denotes and
T25537 17177-17181 NN denotes IL-4
T25538 17182-17183 -LRB- denotes (
T25539 17183-17186 CD denotes 100
T25540 17187-17192 NN denotes ng/ml
T25541 17192-17193 -RRB- denotes )
T25542 17194-17196 IN denotes as
T25543 17197-17206 VBN denotes indicated
T25544 17206-17207 . denotes .
T25545 17208-17213 IN denotes After
T25546 17214-17215 CD denotes 1
T25547 17216-17217 NN denotes h
T25548 17217-17218 , denotes ,
T25549 17219-17223 NN denotes cell
T25550 17224-17231 NNS denotes lysates
T25551 17232-17236 VBD denotes were
T25552 17237-17245 VBN denotes prepared
T25553 17246-17249 CC denotes and
T25554 17250-17258 VBN denotes analyzed
T25555 17259-17261 IN denotes by
T25556 17262-17269 JJ denotes Western
T25557 17270-17274 NN denotes blot
T25558 17275-17278 IN denotes for
T25559 17279-17293 VBN denotes phosphorylated
T25560 17294-17299 NNP denotes SMAD2
T25561 17300-17303 CC denotes and
T25562 17304-17309 NNP denotes STAT6
T25563 17309-17310 . denotes .
T25564 17311-17316 JJ denotes Total
T25565 17317-17322 NNP denotes STAT6
T25566 17323-17326 CC denotes and
T25567 17327-17332 NNP denotes GAPDH
T25568 17333-17339 VBD denotes served
T25569 17340-17342 IN denotes as
T25570 17343-17351 JJ denotes internal
T25571 17352-17359 NN denotes control
T25572 17359-17360 . denotes .
T25573 17361-17362 -LRB- denotes (
T25574 17362-17363 NNP denotes D
T25575 17363-17364 -RRB- denotes )
T25576 17365-17378 NNP denotes Intracellular
T25577 17379-17384 NNP denotes GATA3
T25578 17385-17388 CC denotes and
T25579 17389-17394 NNP denotes FOXP3
T25580 17395-17403 VBG denotes staining
T25581 17404-17407 VBP denotes are
T25582 17408-17413 VBN denotes shown
T25583 17414-17419 IN denotes after
T25584 17420-17428 NN denotes exposure
T25585 17429-17431 IN denotes of
T25586 17432-17435 NNP denotes CD4
T25587 17435-17436 NNP denotes +
T25588 17436-17442 NNP denotes CD45RA
T25589 17442-17443 VBD denotes +
T25590 17444-17445 NNP denotes T
T25591 17446-17451 NNS denotes cells
T25592 17452-17454 TO denotes to
T25593 17455-17459 NN denotes IL-4
T25594 17460-17462 IN denotes as
T25595 17463-17472 VBN denotes described
T25596 17473-17476 IN denotes for
T25597 17477-17482 NN denotes panel
T25598 17483-17485 NNP denotes B.
T25599 17486-17490 NNP denotes Data
T25600 17491-17494 VBP denotes are
T25601 17495-17509 JJ denotes representative
T25602 17510-17512 IN denotes of
T25603 17513-17518 CD denotes three
T25604 17519-17530 JJ denotes independent
T25605 17531-17542 NNS denotes experiments
T25606 17542-17543 . denotes .
T26012 18665-18669 NN denotes IL-4
T26013 18670-18678 NNS denotes Inhibits
T26014 18679-18684 JJ denotes TGF-β
T26015 18685-18693 NNP denotes Mediated
T26016 18694-18699 NNP denotes iTreg
T26017 18700-18710 NNP denotes Commitment
T26018 18711-18723 NNP denotes CFSE-labeled
T26019 18724-18727 NNP denotes CD4
T26020 18727-18728 NNP denotes +
T26021 18728-18734 NNP denotes CD45RA
T26022 18734-18735 VBD denotes +
T26023 18736-18741 NNS denotes cells
T26024 18742-18746 VBD denotes were
T26025 18747-18756 VBN denotes activated
T26026 18757-18761 IN denotes with
T26027 18762-18773 JJ denotes plate-bound
T26028 18774-18782 JJ denotes anti-CD3
T26029 18782-18783 NN denotes /
T26030 18783-18787 NNP denotes CD28
T26031 18787-18788 , denotes ,
T26032 18789-18794 NNP denotes TGF-β
T26033 18794-18795 , denotes ,
T26034 18796-18799 CC denotes and
T26035 18800-18804 NN denotes IL-4
T26036 18804-18805 , denotes ,
T26037 18806-18808 IN denotes as
T26038 18809-18818 VBN denotes indicated
T26039 18818-18819 . denotes .
T26040 18820-18825 IN denotes After
T26041 18826-18827 CD denotes 5
T26042 18828-18829 LS denotes d
T26043 18829-18830 , denotes ,
T26044 18831-18836 NNS denotes cells
T26045 18837-18841 VBD denotes were
T26046 18842-18850 VBN denotes analyzed
T26047 18851-18853 IN denotes by
T26048 18854-18858 NN denotes flow
T26049 18859-18868 NN denotes cytometry
T26050 18869-18870 -LRB- denotes (
T26051 18870-18871 NNP denotes A
T26052 18871-18872 -RRB- denotes )
T26053 18873-18876 CC denotes and
T26054 18877-18884 NNS denotes results
T26055 18885-18887 IN denotes of
T26056 18888-18891 CD denotes six
T26057 18892-18903 JJ denotes independent
T26058 18904-18915 NNS denotes experiments
T26059 18916-18919 VBP denotes are
T26060 18920-18925 VBN denotes shown
T26061 18926-18928 IN denotes in
T26062 18929-18932 DT denotes the
T26063 18933-18936 NN denotes bar
T26064 18937-18942 NN denotes graph
T26065 18943-18948 IN denotes below
T26066 18949-18950 -LRB- denotes (
T26067 18950-18951 NNP denotes B
T26068 18951-18952 -RRB- denotes )
T26069 18952-18953 . denotes .
T26070 18954-18965 NNP denotes Statistical
T26071 18966-18978 NN denotes significance
T26072 18979-18980 -LRB- denotes (
T26073 18980-18987 JJ denotes one-way
T26074 18988-18993 NNP denotes Anova
T26075 18993-18994 , denotes ,
T26076 18995-19007 NNP denotes Newman-Keuls
T26077 19007-19008 -RRB- denotes )
T26078 19009-19011 VBZ denotes is
T26079 19012-19021 VBN denotes indicated
T26080 19022-19024 IN denotes by
T26081 19025-19034 NNS denotes asterisks
T26082 19035-19036 -LRB- denotes (
T26083 19036-19038 SYM denotes **
T26084 19038-19039 FW denotes p
T26085 19040-19041 FW denotes ≤
T26086 19042-19046 CD denotes 0.01
T26087 19046-19047 , denotes ,
T26088 19048-19051 FW denotes ***
T26089 19051-19052 FW denotes p
T26090 19053-19054 FW denotes ≤
T26091 19055-19060 CD denotes 0.001
T26092 19060-19061 -RRB- denotes )
T26093 19061-19062 . denotes .
T26094 19063-19064 -LRB- denotes (
T26095 19064-19065 $ denotes C
T26096 19065-19066 -RRB- denotes )
T26097 19067-19074 JJ denotes Kinetic
T26098 19075-19083 NN denotes analysis
T26099 19084-19086 IN denotes of
T26100 19087-19100 JJ denotes intracellular
T26101 19101-19106 NNP denotes GATA3
T26102 19107-19110 CC denotes and
T26103 19111-19116 NNP denotes FOXP3
T26104 19117-19125 NN denotes staining
T26105 19126-19128 VBZ denotes is
T26106 19129-19134 VBN denotes shown
T26107 19135-19137 IN denotes in
T26108 19138-19143 NN denotes panel
T26109 19144-19145 NNP denotes C
T26110 19146-19155 VBG denotes following
T26111 19156-19164 NN denotes exposure
T26112 19165-19167 IN denotes of
T26113 19168-19171 NNP denotes CD4
T26114 19171-19172 NNP denotes +
T26115 19172-19178 NNP denotes CD45RA
T26116 19178-19179 VBD denotes +
T26117 19180-19181 NNP denotes T
T26118 19182-19187 NNS denotes cells
T26119 19188-19190 TO denotes to
T26120 19191-19199 JJ denotes anti-CD3
T26121 19199-19200 NN denotes /
T26122 19200-19202 CD denotes 28
T26123 19202-19203 , denotes ,
T26124 19204-19208 NN denotes IL-4
T26125 19209-19212 CC denotes and
T26126 19213-19218 NN denotes TGF-β
T26127 19218-19219 . denotes .
T26128 19220-19224 NNS denotes Data
T26129 19225-19228 VBP denotes are
T26130 19229-19243 NN denotes representative
T26131 19244-19246 IN denotes of
T26132 19247-19252 CD denotes three
T26133 19253-19264 JJ denotes independent
T26134 19265-19276 NNS denotes experiments
T26135 19276-19277 . denotes .
T26528 22450-22455 CD denotes GATA3
T26529 22456-22460 NNS denotes Acts
T26530 22461-22463 IN denotes as
T26531 22464-22465 DT denotes a
T26532 22466-22474 JJ denotes Negative
T26533 22475-22484 NNP denotes Regulator
T26534 22485-22487 IN denotes of
T26535 22488-22493 NNP denotes FOXP3
T26536 22494-22504 NNP denotes Expression
T26537 22505-22506 -LRB- denotes (
T26538 22506-22507 NNP denotes A
T26539 22507-22508 -RRB- denotes )
T26540 22509-22514 NNP denotes Human
T26541 22515-22520 JJ denotes naive
T26542 22521-22524 NNP denotes CD4
T26543 22524-22525 CD denotes +
T26544 22525-22529 NNP denotes CDRA
T26545 22529-22530 CD denotes +
T26546 22531-22532 NNP denotes T
T26547 22533-22538 NNS denotes cells
T26548 22539-22543 VBD denotes were
T26549 22544-22554 VBN denotes transduced
T26550 22555-22559 IN denotes with
T26551 22560-22561 CD denotes 0
T26552 22561-22562 , denotes ,
T26553 22563-22565 CD denotes 20
T26554 22565-22566 , denotes ,
T26555 22567-22570 CD denotes 100
T26556 22570-22571 , denotes ,
T26557 22572-22575 CC denotes and
T26558 22576-22579 CD denotes 500
T26559 22580-22582 NN denotes nM
T26560 22583-22585 IN denotes of
T26561 22586-22595 CD denotes TAT-GATA3
T26562 22596-22603 NN denotes protein
T26563 22603-22604 , denotes ,
T26564 22605-22608 CC denotes and
T26565 22609-22622 JJ denotes intracellular
T26566 22623-22631 NN denotes presence
T26567 22632-22634 IN denotes of
T26568 22635-22640 NNP denotes GATA3
T26569 22641-22644 VBD denotes was
T26570 22645-22653 VBN denotes analyzed
T26571 22654-22659 VBG denotes using
T26572 22660-22664 NNP denotes FACS
T26573 22665-22674 VBG denotes following
T26574 22675-22683 JJ denotes anti-CD3
T26575 22683-22684 NN denotes /
T26576 22684-22688 CD denotes CD28
T26577 22689-22699 NN denotes activation
T26578 22700-22702 IN denotes of
T26579 22703-22706 DT denotes the
T26580 22707-22712 NNS denotes cells
T26581 22712-22713 . denotes .
T26582 22714-22726 JJ denotes GFP-positive
T26583 22727-22732 NNS denotes cells
T26584 22733-22737 VBD denotes were
T26585 22738-22743 VBN denotes gated
T26586 22744-22747 CC denotes and
T26587 22748-22756 VBN denotes analyzed
T26588 22757-22760 IN denotes for
T26589 22761-22774 JJ denotes intracellular
T26590 22775-22780 CD denotes FOXP3
T26591 22781-22791 NN denotes expression
T26592 22792-22801 VBG denotes following
T26593 22802-22803 DT denotes a
T26594 22804-22807 JJ denotes 2-d
T26595 22808-22818 NN denotes incubation
T26596 22819-22825 NN denotes period
T26597 22826-22827 -LRB- denotes (
T26598 22827-22832 JJR denotes lower
T26599 22833-22838 NN denotes panel
T26600 22838-22839 -RRB- denotes )
T26601 22839-22840 . denotes .
T26602 22841-22845 NNS denotes Data
T26603 22846-22849 VBP denotes are
T26604 22850-22864 NN denotes representative
T26605 22865-22867 IN denotes of
T26606 22868-22872 CD denotes four
T26607 22873-22884 JJ denotes independent
T26608 22885-22896 NNS denotes experiments
T26609 22896-22897 . denotes .
T26610 22898-22899 -LRB- denotes (
T26611 22899-22900 NNP denotes B
T26612 22900-22901 -RRB- denotes )
T26613 22902-22905 NNP denotes CD4
T26614 22905-22906 NNP denotes +
T26615 22906-22910 NNP denotes CD25
T26616 22910-22911 VBD denotes −
T26617 22912-22913 NNP denotes T
T26618 22914-22919 NNS denotes cells
T26619 22920-22924 VBD denotes were
T26620 22925-22933 VBN denotes isolated
T26621 22934-22938 IN denotes from
T26622 22939-22943 CD denotes D011
T26623 22943-22946 CD denotes .10
T26624 22947-22950 CC denotes and
T26625 22951-22968 JJ denotes D011.10xCD2-GATA3
T26626 22969-22973 NNS denotes mice
T26627 22974-22977 CC denotes and
T26628 22978-22985 VBN denotes treated
T26629 22986-22990 IN denotes with
T26630 22991-22994 NNP denotes OVA
T26631 22995-22998 CC denotes and
T26632 22999-23004 NNP denotes TGF-β
T26633 23005-23008 IN denotes for
T26634 23009-23011 CD denotes 96
T26635 23012-23014 NN denotes h.
T26636 23015-23022 NNP denotes Surface
T26637 23023-23026 NNP denotes CD4
T26638 23027-23030 CC denotes and
T26639 23031-23044 JJ denotes intracellular
T26640 23045-23050 CD denotes FOXP3
T26641 23051-23055 VBD denotes were
T26642 23056-23064 VBN denotes measured
T26643 23065-23067 IN denotes by
T26644 23068-23072 NNP denotes FACS
T26645 23072-23073 . denotes .
T26646 23074-23079 DT denotes These
T26647 23080-23084 NNS denotes data
T26648 23085-23088 VBP denotes are
T26649 23089-23103 NN denotes representative
T26650 23104-23106 IN denotes of
T26651 23107-23112 CD denotes three
T26652 23113-23124 JJ denotes independent
T26653 23125-23136 NNS denotes experiments
T26654 23136-23137 . denotes .
T26655 23138-23139 -LRB- denotes (
T26656 23139-23140 $ denotes C
T26657 23140-23141 -RRB- denotes )
T26658 23142-23145 DT denotes The
T26659 23146-23151 NNS denotes cells
T26660 23152-23159 VBN denotes treated
T26661 23160-23162 IN denotes as
T26662 23163-23165 IN denotes in
T26663 23166-23167 -LRB- denotes (
T26664 23167-23168 NNP denotes B
T26665 23168-23169 -RRB- denotes )
T26666 23170-23174 VBD denotes were
T26667 23175-23184 VBN denotes harvested
T26668 23185-23188 CC denotes and
T26669 23189-23193 NNP denotes mRNA
T26670 23194-23197 VBD denotes was
T26671 23198-23208 VBN denotes quantified
T26672 23209-23211 IN denotes by
T26673 23212-23221 JJ denotes real-time
T26674 23222-23225 NNP denotes PCR
T26675 23226-23229 IN denotes for
T26676 23230-23235 NNP denotes SMAD7
T26677 23236-23239 CC denotes and
T26678 23240-23245 NNP denotes TGF-β
T26679 23246-23256 NN denotes expression
T26680 23256-23257 . denotes .
T26681 23258-23262 NNS denotes Bars
T26682 23263-23267 VBP denotes show
T26683 23268-23271 DT denotes the
T26684 23272-23276 JJ denotes mean
T26685 23277-23278 NN denotes ±
T26686 23279-23281 NNP denotes SD
T26687 23282-23284 IN denotes of
T26688 23285-23290 CD denotes three
T26689 23291-23302 JJ denotes independent
T26690 23303-23314 NNS denotes experiments
T26691 23314-23315 . denotes .
T27256 25937-25942 CD denotes GATA3
T27257 25943-25952 NNPS denotes Represses
T27258 25953-25956 DT denotes the
T27259 25957-25962 NNP denotes Human
T27260 25963-25968 NNP denotes FOXP3
T27261 25969-25977 NNP denotes Promoter
T27262 25978-25979 -LRB- denotes (
T27263 25979-25980 NNP denotes A
T27264 25980-25981 -RRB- denotes )
T27265 25982-25988 NNP denotes Jurkat
T27266 25988-25989 , denotes ,
T27267 25990-25993 NNP denotes Th2
T27268 25994-25999 NNS denotes cells
T27269 25999-26000 , denotes ,
T27270 26001-26004 CC denotes and
T27271 26005-26010 JJ denotes human
T27272 26011-26018 JJ denotes primary
T27273 26019-26022 NNP denotes CD4
T27274 26023-26028 NNS denotes cells
T27275 26029-26033 VBD denotes were
T27276 26034-26045 VBN denotes transfected
T27277 26046-26050 IN denotes with
T27278 26051-26053 DT denotes an
T27279 26054-26059 JJ denotes empty
T27280 26060-26066 NN denotes vector
T27281 26067-26068 -LRB- denotes (
T27282 26068-26072 JJ denotes pGL3
T27283 26073-26078 JJ denotes basic
T27284 26078-26079 -RRB- denotes )
T27285 26080-26082 CC denotes or
T27286 26083-26084 DT denotes a
T27287 26085-26091 NN denotes vector
T27288 26092-26102 VBG denotes containing
T27289 26103-26106 DT denotes the
T27290 26107-26115 JJ denotes putative
T27291 26116-26121 CD denotes FOXP3
T27292 26122-26130 NN denotes promoter
T27293 26131-26137 NN denotes region
T27294 26138-26143 VBD denotes fused
T27295 26144-26146 TO denotes to
T27296 26147-26150 DT denotes the
T27297 26151-26161 NN denotes luciferase
T27298 26162-26170 NN denotes reporter
T27299 26171-26175 NN denotes gene
T27300 26175-26176 . denotes .
T27301 26177-26181 NNS denotes Bars
T27302 26182-26186 VBP denotes show
T27303 26187-26190 DT denotes the
T27304 26191-26195 JJ denotes mean
T27305 26196-26197 NN denotes ±
T27306 26198-26200 NNP denotes SD
T27307 26201-26203 IN denotes of
T27308 26204-26213 JJ denotes arbitrary
T27309 26214-26219 JJ denotes light
T27310 26220-26225 NNS denotes units
T27311 26226-26236 VBN denotes normalized
T27312 26237-26240 IN denotes for
T27313 26241-26248 NN denotes renilla
T27314 26249-26259 NN denotes luciferase
T27315 26260-26262 IN denotes of
T27316 26263-26267 CD denotes four
T27317 26268-26279 JJ denotes independent
T27318 26280-26291 NNS denotes experiments
T27319 26291-26292 : denotes ;
T27320 26293-26300 NNS denotes samples
T27321 26301-26305 VBD denotes were
T27322 26306-26314 VBN denotes measured
T27323 26315-26317 IN denotes in
T27324 26318-26329 NNS denotes triplicates
T27325 26329-26330 . denotes .
T27326 26331-26332 -LRB- denotes (
T27327 26332-26333 NNP denotes B
T27328 26333-26334 -RRB- denotes )
T27329 26335-26340 NNP denotes Naive
T27330 26341-26344 NNP denotes CD4
T27331 26345-26346 NNP denotes T
T27332 26347-26352 NNS denotes cells
T27333 26353-26357 VBD denotes were
T27334 26358-26369 VBN denotes transfected
T27335 26370-26374 IN denotes with
T27336 26375-26378 DT denotes the
T27337 26379-26384 CD denotes FOXP3
T27338 26385-26393 NN denotes promoter
T27339 26394-26402 NN denotes reporter
T27340 26403-26412 VB denotes construct
T27341 26413-26421 RB denotes together
T27342 26422-26426 IN denotes with
T27343 26427-26428 DT denotes a
T27344 26429-26434 JJ denotes GATA3
T27345 26435-26445 NN denotes expression
T27346 26446-26452 NN denotes vector
T27347 26453-26455 CC denotes or
T27348 26456-26458 DT denotes an
T27349 26459-26464 JJ denotes empty
T27350 26465-26471 NN denotes vector
T27351 26471-26472 . denotes .
T27352 26473-26477 NNS denotes Bars
T27353 26478-26482 VBP denotes show
T27354 26483-26486 DT denotes the
T27355 26487-26491 JJ denotes mean
T27356 26492-26493 NN denotes ±
T27357 26494-26496 NNP denotes SD
T27358 26497-26499 IN denotes of
T27359 26500-26505 CD denotes three
T27360 26506-26517 JJ denotes independent
T27361 26518-26529 NNS denotes experiments
T27362 26529-26530 . denotes .
T27363 26531-26532 -LRB- denotes (
T27364 26532-26533 NNP denotes C
T27365 26533-26534 -RRB- denotes )
T27366 26535-26540 NNP denotes Naive
T27367 26541-26542 -LRB- denotes (
T27368 26542-26546 VBN denotes left
T27369 26547-26552 NN denotes panel
T27370 26552-26553 -RRB- denotes )
T27371 26554-26556 CC denotes or
T27372 26557-26563 NN denotes memory
T27373 26564-26565 -LRB- denotes (
T27374 26565-26570 JJ denotes right
T27375 26571-26576 NN denotes panel
T27376 26576-26577 -RRB- denotes )
T27377 26578-26581 CD denotes CD4
T27378 26582-26583 NNP denotes T
T27379 26584-26589 NNS denotes cells
T27380 26590-26594 VBD denotes were
T27381 26595-26606 VBN denotes transfected
T27382 26607-26611 IN denotes with
T27383 26612-26621 JJ denotes wild-type
T27384 26622-26624 CC denotes or
T27385 26625-26626 DT denotes a
T27386 26627-26632 NNP denotes GATA3
T27387 26633-26640 VBD denotes mutated
T27388 26641-26650 JJ denotes 511-FOXP3
T27389 26651-26659 NN denotes promoter
T27390 26660-26668 NN denotes reporter
T27391 26669-26678 VB denotes construct
T27392 26679-26682 CC denotes and
T27393 26683-26692 VBN denotes activated
T27394 26693-26697 IN denotes with
T27395 26698-26701 NNP denotes PMA
T27396 26702-26705 CC denotes and
T27397 26706-26715 NN denotes ionomycin
T27398 26715-26716 . denotes .
T27399 26717-26721 NNS denotes Bars
T27400 26722-26726 VBP denotes show
T27401 26727-26730 DT denotes the
T27402 26731-26735 JJ denotes mean
T27403 26736-26737 NN denotes ±
T27404 26738-26740 NNP denotes SD
T27405 26741-26743 IN denotes of
T27406 26744-26753 JJ denotes arbitrary
T27407 26754-26759 JJ denotes light
T27408 26760-26765 NNS denotes units
T27409 26766-26776 VBN denotes normalized
T27410 26777-26780 IN denotes for
T27411 26781-26788 NN denotes renilla
T27412 26789-26799 NN denotes luciferase
T27413 26800-26802 IN denotes of
T27414 26803-26808 CD denotes eight
T27415 26809-26820 JJ denotes independent
T27416 26821-26832 NNS denotes experiments
T27417 26832-26833 : denotes ;
T27418 26834-26841 NNS denotes samples
T27419 26842-26846 VBD denotes were
T27420 26847-26855 VBN denotes measured
T27421 26856-26858 IN denotes in
T27422 26859-26870 NNS denotes triplicates
T27423 26870-26871 . denotes .
T27424 26872-26873 -LRB- denotes (
T27425 26873-26874 NNP denotes D
T27426 26874-26875 -RRB- denotes )
T27427 26876-26883 NNP denotes Nuclear
T27428 26884-26892 NNS denotes extracts
T27429 26893-26897 VBD denotes were
T27430 26898-26906 VBN denotes prepared
T27431 26907-26911 IN denotes from
T27432 26912-26915 NNP denotes HEK
T27433 26916-26921 NNS denotes cells
T27434 26922-26933 VBN denotes transfected
T27435 26934-26938 IN denotes with
T27436 26939-26944 CD denotes GATA3
T27437 26945-26947 CC denotes or
T27438 26948-26950 DT denotes an
T27439 26951-26956 JJ denotes empty
T27440 26957-26963 NN denotes vector
T27441 26963-26964 , denotes ,
T27442 26965-26966 -LRB- denotes (
T27443 26966-26967 NNP denotes E
T27444 26967-26968 -RRB- denotes )
T27445 26969-26972 NNP denotes Th1
T27446 26972-26973 , denotes ,
T27447 26974-26977 NNP denotes Th2
T27448 26977-26978 , denotes ,
T27449 26979-26981 CC denotes or
T27450 26982-26987 NNP denotes iTreg
T27451 26988-26993 NNS denotes cells
T27452 26994-26997 CC denotes and
T27453 26998-27005 JJ denotes binding
T27454 27006-27013 NNS denotes factors
T27455 27014-27026 VBD denotes precipitated
T27456 27027-27032 VBG denotes using
T27457 27033-27045 JJ denotes biotinylated
T27458 27046-27062 NNS denotes oligonucleotides
T27459 27062-27063 . denotes .
T27460 27064-27067 DT denotes The
T27461 27068-27084 NNS denotes oligonucleotides
T27462 27085-27098 NN denotes transcription
T27463 27099-27105 NN denotes factor
T27475 27165-27170 NNP denotes GATA3
T27476 27171-27178 NN denotes protein
T27477 27179-27181 IN denotes in
T27478 27182-27185 DT denotes the
T27479 27186-27198 NNS denotes precipitates
T27480 27199-27203 VBD denotes were
T27481 27204-27212 VBN denotes assessed
T27482 27213-27215 IN denotes by
T27483 27216-27230 VBG denotes immunoblotting
T27484 27231-27235 IN denotes with
T27485 27236-27246 JJ denotes anti-GATA3
T27486 27247-27250 NNP denotes mAb
T27487 27250-27251 . denotes .
T27488 27252-27257 JJ denotes Total
T27489 27258-27265 JJ denotes nuclear
T27490 27266-27274 NNS denotes extracts
T27491 27275-27279 VBD denotes were
T27492 27280-27284 RB denotes also
T27493 27285-27288 VBN denotes run
T27494 27289-27291 IN denotes as
T27495 27292-27300 NNS denotes controls
T27496 27300-27301 . denotes .
T27497 27302-27306 NNS denotes Data
T27498 27307-27310 VBP denotes are
T27499 27311-27325 NN denotes representative
T27500 27326-27328 IN denotes of
T27501 27329-27334 CD denotes three
T27502 27335-27344 JJ denotes different
T27503 27345-27356 NNS denotes experiments
T27504 27356-27357 . denotes .
T27505 27358-27359 -LRB- denotes (
T27506 27359-27360 NN denotes F
T27507 27360-27361 -RRB- denotes )
T27508 27362-27367 NN denotes iTreg
T27509 27368-27370 CC denotes or
T27510 27371-27374 JJ denotes Th2
T27511 27375-27380 NNS denotes cells
T27512 27381-27385 VBD denotes were
T27513 27386-27394 VBN denotes analyzed
T27514 27395-27397 IN denotes by
T27515 27398-27402 NNP denotes ChIP
T27516 27403-27406 IN denotes for
T27517 27407-27412 NNP denotes GATA3
T27518 27413-27420 JJ denotes binding
T27519 27421-27423 TO denotes to
T27520 27424-27427 DT denotes the
T27521 27428-27433 CD denotes FOXP3
T27522 27434-27442 NN denotes promoter
T27523 27442-27443 . denotes .
T27524 27444-27447 DT denotes The
T27525 27449-27454 NN denotes input
T27526 27456-27466 VBZ denotes represents
T27527 27467-27470 NNP denotes PCR
T27528 27471-27484 NN denotes amplification
T27529 27485-27487 IN denotes of
T27530 27488-27491 DT denotes the
T27531 27492-27497 JJ denotes total
T27532 27498-27504 NN denotes sample
T27533 27504-27505 , denotes ,
T27534 27506-27511 WDT denotes which
T27535 27512-27515 VBD denotes was
T27536 27516-27519 RB denotes not
T27537 27520-27529 VBN denotes subjected
T27538 27530-27532 TO denotes to
T27539 27533-27536 DT denotes any
T27540 27537-27550 NN denotes precipitation
T27541 27550-27551 . denotes .
T27542 27552-27559 NNS denotes Results
T27543 27560-27563 VBP denotes are
T27544 27564-27578 NN denotes representative
T27545 27579-27581 IN denotes of
T27546 27582-27587 CD denotes three
T27547 27588-27599 JJ denotes independent
T27548 27600-27611 NNS denotes experiments
T27549 27611-27612 . denotes .
T27464 27106-27115 NNS denotes complexes
T27465 27116-27120 VBD denotes were
T27466 27121-27130 VBN denotes separated
T27467 27131-27133 IN denotes on
T27468 27134-27135 DT denotes a
T27469 27136-27144 NNP denotes SDS-PAGE
T27470 27145-27148 NN denotes gel
T27471 27148-27149 . denotes .
T27472 27150-27153 DT denotes The
T27473 27154-27161 NNS denotes amounts
T27474 27162-27164 IN denotes of
R281 T361 T363 nummod GATA3-Driven,Responses
R282 T362 T363 compound Th2,Responses
R283 T363 T364 nsubj Responses,Inhibit
R284 T364 T364 ROOT Inhibit,Inhibit
R285 T365 T364 dobj TGF-β1,Inhibit
R286 T366 T368 compound Induced,Expression
R287 T367 T368 compound FOXP3,Expression
R288 T368 T378 nsubj Expression,act
R289 T369 T368 cc and,Expression
R290 T370 T371 det the,Formation
R291 T371 T368 conj Formation,Expression
R292 T372 T371 prep of,Formation
R293 T373 T376 compound Regulatory,Transcription
R294 T374 T376 compound T,Transcription
R295 T375 T376 compound Cells,Transcription
R296 T376 T377 compound Transcription,factors
R297 T377 T372 pobj factors,of
R298 T378 T364 conj act,Inhibit
R299 T379 T378 prep in,act
R300 T380 T379 pobj concert,in
R301 T381 T382 aux to,induce
R302 T382 T378 advcl induce,act
R303 T383 T384 compound lineage,commitment
R304 T384 T382 dobj commitment,induce
R305 T385 T384 prep towards,commitment
R306 T386 T385 pobj Th1,towards
R307 T387 T386 punct ",",Th1
R308 T388 T386 conj Th2,Th1
R309 T389 T388 punct ",",Th2
R310 T390 T388 cc or,Th2
R311 T391 T388 conj T,Th2
R312 T392 T396 amod regulatory,cells
R313 T393 T394 punct (,Treg
R314 T394 T392 appos Treg,regulatory
R315 T395 T394 punct ),Treg
R316 T396 T385 pobj cells,towards
R317 T397 T378 punct ",",act
R318 T398 T378 cc and,act
R319 T399 T401 poss their,mechanisms
R320 T400 T401 amod counter-regulatory,mechanisms
R321 T401 T403 nsubjpass mechanisms,shown
R378 T458 T454 relcl overruled,polarization
R379 T459 T458 agent by,overruled
R380 T460 T463 det the,pathway
R381 T461 T463 amod Th2,pathway
R382 T462 T463 compound differentiation,pathway
R383 T463 T459 pobj pathway,by
R384 T464 T449 punct .,describe
R385 T465 T466 nsubj We,demonstrated
R386 T466 T466 ROOT demonstrated,demonstrated
R387 T467 T481 mark that,inhibits
R388 T468 T481 nsubj interleukin,inhibits
R389 T469 T468 nummod 4,interleukin
R390 T470 T471 punct (,IL-4
R391 T471 T468 appos IL-4,interleukin
R392 T472 T471 punct ),IL-4
R393 T473 T468 amod present,interleukin
R394 T474 T468 prep at,interleukin
R395 T475 T476 det the,time
R396 T476 T474 pobj time,at
R397 T477 T476 prep of,time
R398 T478 T479 compound T,cell
R399 T479 T480 compound cell,priming
R400 T480 T481 nsubj priming,inhibits
R401 T481 T466 ccomp inhibits,demonstrated
R402 T482 T481 dobj FOXP3,inhibits
R403 T483 T466 punct .,demonstrated
R404 T484 T486 det This,mechanism
R405 T485 T486 amod inhibitory,mechanism
R406 T486 T489 nsubjpass mechanism,confirmed
R407 T487 T489 auxpass was,confirmed
R408 T488 T489 advmod also,confirmed
R409 T489 T489 ROOT confirmed,confirmed
R410 T490 T489 prep in,confirmed
R411 T491 T492 amod Th2,cells
R412 T492 T490 pobj cells,in
R413 T493 T490 cc and,in
R414 T494 T490 conj in,in
R415 T495 T496 compound T,cells
R416 T496 T494 pobj cells,in
R417 T497 T496 prep of,cells
R418 T498 T499 amod transgenic,mice
R419 T499 T497 pobj mice,of
R420 T500 T489 advcl overexpressing,confirmed
R421 T501 T500 dobj GATA-3,overexpressing
R422 T502 T500 prep in,overexpressing
R423 T503 T504 compound T,cells
R424 T504 T502 pobj cells,in
R425 T505 T489 punct ",",confirmed
R426 T506 T508 nsubjpass which,shown
R427 T507 T508 auxpass are,shown
R428 T508 T489 advcl shown,confirmed
R429 T509 T510 aux to,be
R430 T510 T508 xcomp be,shown
R431 T511 T510 acomp deficient,be
R432 T512 T511 prep in,deficient
R433 T513 T512 pcomp transforming,in
R434 T514 T515 compound growth,factor
R435 T515 T513 dobj factor,transforming
R436 T516 T517 punct (,TGF
R437 T517 T515 appos TGF,factor
R438 T518 T517 punct ),TGF
R439 T519 T515 punct -,factor
R440 T520 T515 appos β,factor
R441 T521 T489 conj mediated,confirmed
R442 T522 T523 nummod FOXP3,induction
R443 T523 T521 dobj induction,mediated
R444 T524 T489 punct .,confirmed
R445 T525 T526 det This,inhibition
R446 T526 T528 nsubjpass inhibition,mediated
R447 T527 T528 auxpass is,mediated
R448 T528 T528 ROOT mediated,mediated
R449 T529 T528 agent by,mediated
R450 T530 T531 amod direct,binding
R451 T531 T529 pobj binding,by
R452 T532 T531 prep of,binding
R453 T533 T532 pobj GATA3,of
R454 T534 T528 prep to,mediated
R455 T535 T537 det the,promoter
R456 T536 T537 nummod FOXP3,promoter
R457 T537 T534 pobj promoter,to
R458 T538 T537 punct ",",promoter
R459 T539 T540 nsubj which,represses
R460 T540 T537 relcl represses,promoter
R461 T541 T543 poss its,process
R462 T542 T543 compound transactivation,process
R463 T543 T540 dobj process,represses
R464 T544 T528 punct .,mediated
R465 T545 T549 advmod Therefore,provides
R466 T546 T549 punct ",",provides
R467 T547 T548 det this,study
R468 T548 T549 nsubj study,provides
R469 T549 T549 ROOT provides,provides
R470 T550 T552 det a,understanding
R471 T551 T552 amod new,understanding
R472 T552 T549 dobj understanding,provides
R473 T553 T552 prep of,understanding
R474 T554 T555 compound tolerance,development
R475 T555 T553 pobj development,of
R476 T556 T552 punct ",",understanding
R477 T557 T552 acl controlled,understanding
R478 T558 T557 agent by,controlled
R479 T559 T563 det a,response
R480 T560 T563 nmod type,response
R481 T561 T560 nummod 2,type
R482 T562 T563 amod immune,response
R483 T563 T558 pobj response,by
R484 T564 T549 punct .,provides
R485 T565 T566 compound IL-4,treatment
R486 T566 T569 nsubj treatment,reduces
R487 T567 T566 prep in,treatment
R488 T568 T567 pobj mice,in
R489 T569 T569 ROOT reduces,reduces
R490 T570 T572 compound iTreg,frequency
R491 T571 T572 compound cell,frequency
R492 T572 T569 dobj frequency,reduces
R493 T573 T569 punct ",",reduces
R494 T574 T569 advcl highlighting,reduces
R495 T575 T584 mark that,provide
R496 T576 T577 amod therapeutic,approaches
R497 T577 T584 nsubj approaches,provide
R498 T578 T579 nsubj that,target
R499 T579 T577 relcl target,approaches
R500 T580 T579 dobj IL-4,target
R501 T581 T580 cc or,IL-4
R502 T582 T580 conj GATA3,IL-4
R503 T583 T584 aux might,provide
R504 T584 T574 ccomp provide,highlighting
R505 T585 T587 amod new,strategies
R506 T586 T587 amod preventive,strategies
R507 T587 T584 dobj strategies,provide
R508 T588 T584 advcl facilitating,provide
R509 T589 T590 compound tolerance,induction
R510 T590 T588 dobj induction,facilitating
R511 T591 T592 advmod particularly,in
R512 T592 T590 prep in,induction
R513 T593 T594 amod Th2-mediated,diseases
R514 T594 T592 pobj diseases,in
R515 T595 T594 punct ",",diseases
R516 T596 T597 amod such,as
R517 T597 T594 prep as,diseases
R518 T598 T597 pobj allergy,as
R519 T599 T569 punct .,reduces
R1374 T1634 T1636 amod Effective,responses
R1375 T1635 T1636 amod immune,responses
R1376 T1636 T1638 nsubjpass responses,characterized
R1377 T1637 T1638 auxpass are,characterized
R1378 T1638 T1638 ROOT characterized,characterized
R1379 T1639 T1638 agent by,characterized
R1380 T1640 T1642 compound T,activation
R1381 T1641 T1642 compound cell,activation
R1382 T1642 T1639 pobj activation,by
R1383 T1643 T1642 punct ",",activation
R1384 T1644 T1645 nsubj which,directs
R1385 T1645 T1642 relcl directs,activation
R1386 T1646 T1645 acomp adaptive,directs
R1387 T1647 T1646 cc and,adaptive
R1388 T1648 T1646 conj innate,adaptive
R1389 T1649 T1650 amod immune,responses
R1390 T1650 T1645 dobj responses,directs
R1391 T1651 T1652 aux to,kill
R1392 T1652 T1650 acl kill,responses
R1393 T1653 T1652 dobj pathogens,kill
R1394 T1654 T1652 advmod efficiently,kill
R1395 T1655 T1638 punct .,characterized
R1396 T1656 T1663 advcl Dependent,differentiate
R1397 T1657 T1656 prep on,Dependent
R1398 T1658 T1659 det the,pathogen
R1399 T1659 T1657 pobj pathogen,on
R1400 T1660 T1663 punct ",",differentiate
R1401 T1661 T1662 compound T,cells
R1402 T1662 T1663 nsubj cells,differentiate
R1403 T1663 T1663 ROOT differentiate,differentiate
R1404 T1664 T1663 prep into,differentiate
R1405 T1665 T1666 amod different,subtypes
R1406 T1666 T1664 pobj subtypes,into
R1407 T1667 T1666 punct ",",subtypes
R1408 T1668 T1669 amod such,as
R1409 T1669 T1666 prep as,subtypes
R1445 T1705 T1703 pobj Th1,to
R1446 T1706 T1705 cc or,Th1
R1447 T1707 T1705 conj Th2,Th1
R1448 T1708 T1697 punct ",",exclusiveness
R1449 T1709 T1697 cc but,exclusiveness
R1450 T1710 T1712 neg not,mixed
R1451 T1711 T1712 aux to,mixed
R1452 T1712 T1697 conj mixed,exclusiveness
R1453 T1713 T1712 dobj populations,mixed
R1454 T1714 T1695 punct .,is
R1455 T1715 T1716 det The,exclusiveness
R1456 T1716 T1721 nsubjpass exclusiveness,provided
R1457 T1717 T1716 prep of,exclusiveness
R1458 T1718 T1719 det this,mechanism
R1459 T1719 T1717 pobj mechanism,of
R1460 T1720 T1721 auxpass is,provided
R1461 T1721 T1721 ROOT provided,provided
R1462 T1722 T1721 agent by,provided
R1463 T1723 T1725 det a,process
R1464 T1724 T1725 compound polarization,process
R1465 T1725 T1722 pobj process,by
R1466 T1726 T1725 punct ",",process
R1467 T1727 T1730 advmod where,inhibits
R1468 T1728 T1729 amod Th2,differentiation
R1469 T1729 T1730 nsubj differentiation,inhibits
R1470 T1730 T1725 relcl inhibits,process
R1471 T1731 T1732 amod Th1,commitment
R1472 T1732 T1730 dobj commitment,inhibits
R1473 T1733 T1732 cc and,commitment
R1474 T1734 T1735 advmod vice,versa
R1475 T1735 T1732 conj versa,commitment
R1476 T1736 T1721 punct .,provided
R1477 T1737 T1738 advmod Specifically,interleukin
R1478 T1738 T1738 ROOT interleukin,interleukin
R1479 T1739 T1738 nummod 4,interleukin
R1480 T1740 T1741 punct (,IL-4
R1481 T1741 T1743 nsubj IL-4,induced
R1482 T1742 T1741 punct ),IL-4
R1483 T1743 T1743 ROOT induced,induced
R1484 T1744 T1743 dobj STAT6,induced
R1485 T1745 T1744 cc and,STAT6
R1486 T1746 T1744 conj GATA3,STAT6
R1487 T1747 T1743 conj inhibit,induced
R1488 T1748 T1747 dobj differentiation,inhibit
R1489 T1749 T1748 prep into,differentiation
R1490 T1750 T1751 amod Th1,cells
R1491 T1751 T1749 pobj cells,into
R1492 T1752 T1748 prep in,differentiation
R1493 T1753 T1755 det the,phase
R1494 T1754 T1755 amod early,phase
R1495 T1755 T1752 pobj phase,in
R1496 T1756 T1755 prep of,phase
R1497 T1757 T1756 pobj commitment,of
R1498 T1758 T1760 compound [,]
R1499 T1759 T1760 compound "1,2",]
R1500 T1760 T1760 ROOT ],]
R1501 T1761 T1743 punct .,induced
R1502 T1762 T1763 nsubj GATA3,is
R1503 T1763 T1763 ROOT is,is
R1504 T1764 T1763 acomp sufficient,is
R1505 T1765 T1766 aux to,induce
R1506 T1766 T1764 xcomp induce,sufficient
R1507 T1767 T1774 det a,acts
R1508 T1768 T1770 amod Th2,[
R1509 T1769 T1770 compound phenotype,[
R1510 T1770 T1774 nummod [,acts
R1511 T1771 T1770 nummod 3,[
R1512 T1772 T1770 appos ],[
R1513 T1773 T1770 cc and,[
R1514 T1774 T1766 dobj acts,induce
R1515 T1775 T1777 preconj not,through
R1516 T1776 T1775 advmod only,not
R1517 T1777 T1774 prep through,acts
R1518 T1778 T1779 det the,induction
R1519 T1779 T1777 pobj induction,through
R1520 T1780 T1779 prep of,induction
R1521 T1781 T1780 pobj IL-4,of
R1522 T1782 T1781 punct ",",IL-4
R1523 T1783 T1781 conj IL-5,IL-4
R1524 T1784 T1783 cc and,IL-5
R1525 T1785 T1783 conj IL-13,IL-5
R1526 T1786 T1785 punct ",",IL-13
R1527 T1787 T1789 det the,cytokines
R1528 T1788 T1789 compound Th2,cytokines
R1529 T1789 T1779 appos cytokines,induction
R1530 T1790 T1774 punct ",",acts
R1531 T1791 T1774 cc but,acts
R1532 T1792 T1791 advmod also,but
R1533 T1793 T1766 prep through,induce
R1534 T1794 T1795 det the,inhibition
R1535 T1795 T1793 pobj inhibition,through
R1536 T1796 T1795 prep of,inhibition
R1537 T1797 T1799 nummod Th1,factors
R1538 T1798 T1799 amod cell-specific,factors
R1539 T1799 T1796 pobj factors,of
R1540 T1800 T1802 nmod [,]
R1541 T1801 T1802 nummod 3,]
R1542 T1802 T1763 npadvmod ],is
R1543 T1803 T1763 punct .,is
R1544 T1804 T1808 advmod Recently,shown
R1545 T1805 T1808 punct ",",shown
R1546 T1806 T1808 nsubjpass it,shown
R1547 T1807 T1808 auxpass was,shown
R1548 T1808 T1808 ROOT shown,shown
R1549 T1809 T1812 mark that,modulates
R1550 T1810 T1812 nsubj T-bet,modulates
R1551 T1811 T1812 advmod directly,modulates
R1552 T1812 T1808 ccomp modulates,shown
R1553 T1813 T1814 nummod GATA3,function
R1554 T1814 T1812 dobj function,modulates
R1555 T1815 T1808 punct ",",shown
R1556 T1816 T1808 advcl suggesting,shown
R1557 T1817 T1820 mark that,compete
R1558 T1818 T1819 compound transcription,factors
R1559 T1819 T1820 nsubj factors,compete
R1560 T1820 T1816 ccomp compete,suggesting
R1561 T1821 T1820 prep in,compete
R1562 T1822 T1825 det the,phase
R1563 T1823 T1825 amod early,phase
R1564 T1824 T1825 compound differentiation,phase
R1565 T1825 T1821 pobj phase,in
R1566 T1826 T1825 prep of,phase
R1567 T1827 T1828 compound T,cells
R1568 T1828 T1826 pobj cells,of
R1569 T1829 T1820 punct ",",compete
R1570 T1830 T1831 advmod potentially,integrating
R1571 T1831 T1820 advcl integrating,compete
R1572 T1832 T1833 amod environmental,signals
R1573 T1833 T1831 dobj signals,integrating
R1574 T1834 T1836 aux to,imprint
R1575 T1835 T1836 advmod finally,imprint
R1576 T1836 T1831 advcl imprint,integrating
R1577 T1837 T1840 det the,phenotype
R1578 T1838 T1839 compound T,cell
R1579 T1839 T1840 compound cell,phenotype
R1580 T1840 T1836 dobj phenotype,imprint
R1581 T1841 T1843 compound [,]
R1582 T1842 T1843 compound "4,5",]
R1583 T1843 T1840 appos ],phenotype
R1584 T1844 T1808 punct .,shown
R1585 T1845 T1848 det A,response
R1586 T1846 T1848 amod GATA3-dominated,response
R1587 T1847 T1848 amod immune,response
R1588 T1848 T1851 nsubjpass response,shown
R1589 T1849 T1851 aux has,shown
R1590 T1850 T1851 auxpass been,shown
R1591 T1851 T1851 ROOT shown,shown
R1592 T1852 T1853 aux to,be
R1593 T1853 T1851 xcomp be,shown
R1594 T1854 T1853 acomp essential,be
R1595 T1855 T1853 prep in,be
R1596 T1856 T1855 pobj airway,in
R1597 T1857 T1858 compound hyperresonsiveness,[
R1598 T1858 T1856 dobj [,airway
R1599 T1859 T1860 nummod 6,]
R1600 T1860 T1858 appos ],[
R1601 T1861 T1860 cc and,]
R1602 T1862 T1860 conj IL-4,]
R1603 T1863 T1851 conj dominated,shown
R1604 T1864 T1866 nsubj responses,break
R1605 T1865 T1866 aux can,break
R1606 T1866 T1851 conj break,shown
R1607 T1867 T1869 amod antigen-specific,tolerance
R1608 T1868 T1869 amod immune,tolerance
R1609 T1869 T1866 dobj tolerance,break
R1610 T1870 T1872 nmod [,]
R1611 T1871 T1872 nummod 7,]
R1612 T1872 T1869 appos ],tolerance
R1613 T1873 T1866 punct .,break
R1614 T1874 T1894 nsubj Overexpression,decreased
R1615 T1875 T1874 prep of,Overexpression
R1616 T1876 T1879 det a,form
R1617 T1877 T1879 amod dominant,form
R1618 T1878 T1879 amod negative,form
R1619 T1879 T1875 pobj form,of
R1620 T1880 T1879 prep of,form
R1621 T1881 T1882 compound GATA3,[
R1622 T1882 T1884 nmod [,]
R1623 T1883 T1884 nummod 8,]
R1624 T1884 T1886 nmod ],treatment
R1625 T1885 T1884 cc or,]
R1626 T1886 T1880 pobj treatment,of
R1627 T1887 T1886 prep with,treatment
R1628 T1888 T1891 amod antisense-mediated,[
R1629 T1889 T1890 compound GATA3,blockade
R1630 T1890 T1891 compound blockade,[
R1631 T1891 T1893 nmod [,]
R1632 T1892 T1893 nummod 9,]
R1633 T1893 T1887 pobj ],with
R1634 T1894 T1894 ROOT decreased,decreased
R1635 T1895 T1896 det the,severity
R1636 T1896 T1894 dobj severity,decreased
R1637 T1897 T1896 prep of,severity
R1638 T1898 T1901 det the,hyper-responsiveness
R1639 T1899 T1901 amod allergic,hyper-responsiveness
R1640 T1900 T1901 compound airway,hyper-responsiveness
R1641 T1901 T1897 pobj hyper-responsiveness,of
R1642 T1902 T1894 punct .,decreased
R1643 T1903 T1904 det The,discovery
R1644 T1904 T1912 nsubj discovery,highlights
R1645 T1905 T1904 prep of,discovery
R1646 T1906 T1907 amod regulatory,T
R1647 T1907 T1905 pobj T,of
R1648 T1908 T1909 punct (,Treg
R1649 T1909 T1907 appos Treg,T
R1650 T1910 T1907 punct ),T
R1651 T1911 T1905 pobj cells,of
R1652 T1912 T1912 ROOT highlights,highlights
R1653 T1913 T1914 det another,phenotype
R1654 T1914 T1912 dobj phenotype,highlights
R1655 T1915 T1914 prep of,phenotype
R1656 T1916 T1917 compound T,cells
R1657 T1917 T1915 pobj cells,of
R1658 T1918 T1917 punct ",",cells
R1659 T1919 T1920 nsubj which,is
R1660 T1920 T1914 relcl is,phenotype
R1661 T1921 T1920 acomp essential,is
R1662 T1922 T1920 prep for,is
R1663 T1923 T1922 pobj tolerance,for
R1664 T1924 T1923 prep against,tolerance
R1665 T1925 T1924 pobj self-antigens,against
R1666 T1926 T1912 punct .,highlights
R1667 T1927 T1937 advmod Naturally,generated
R1668 T1928 T1937 advcl occurring,generated
R1669 T1929 T1937 punct ",",generated
R1670 T1930 T1931 compound thymus-derived,Treg
R1671 T1931 T1935 poss Treg,cells
R1672 T1932 T1933 punct (,nTreg
R1673 T1933 T1931 appos nTreg,Treg
R1674 T1934 T1931 punct ),Treg
R1675 T1935 T1937 nsubjpass cells,generated
R1676 T1936 T1937 auxpass are,generated
R1677 T1937 T1937 ROOT generated,generated
R1678 T1938 T1937 prep in,generated
R1679 T1939 T1940 det the,thymus
R1680 T1940 T1938 pobj thymus,in
R1718 T1978 T1981 nummod Th1,cytokines
R1719 T1979 T1978 cc or,Th1
R1720 T1980 T1978 conj Th2,Th1
R1721 T1981 T1977 pobj cytokines,of
R1722 T1982 T1959 punct .,express
R1723 T1983 T1984 advmod Particularly,interesting
R1724 T1984 T1985 acomp interesting,are
R1725 T1985 T1985 ROOT are,are
R1726 T1986 T1988 det those,cells
R1727 T2042 T2043 nummod FOXP3,expression
R1728 T1987 T1988 compound Treg,cells
R1729 T2043 T2041 dobj expression,induce
R1730 T2044 T2043 cc and,expression
R1731 T1988 T1985 nsubj cells,are
R1732 T2045 T2046 amod suppressive,cells
R1733 T2046 T2043 conj cells,expression
R1734 T1989 T1991 nsubjpass that,generated
R1735 T2047 T2041 prep in,induce
R1736 T2048 T2049 amod human,[
R1737 T2049 T2051 compound [,]
R1738 T1990 T1991 auxpass are,generated
R1739 T2050 T2051 compound "11,12",]
R1740 T2051 T2047 pobj ],in
R1741 T2052 T2041 punct ",",induce
R1742 T2053 T2056 advmod however,appears
R1743 T2054 T2055 det the,phenotype
R1744 T2055 T2056 nsubj phenotype,appears
R1745 T2056 T2039 conj appears,shown
R1746 T1991 T1988 relcl generated,cells
R1747 T2057 T2058 aux to,be
R1748 T2058 T2056 xcomp be,appears
R1749 T2059 T2058 prep of,be
R1750 T1992 T1991 prep in,generated
R1751 T2060 T2061 amod transient,nature
R1752 T2061 T2059 pobj nature,of
R1753 T2062 T2064 compound [,]
R1754 T2063 T2064 compound "13,14",]
R1755 T1993 T1994 det the,periphery
R1756 T2064 T2056 oprd ],appears
R1757 T2065 T2056 punct .,appears
R1758 T2066 T2069 nsubjpass TGF-β,demonstrated
R1759 T1994 T1992 pobj periphery,in
R1760 T2067 T2069 aux has,demonstrated
R1761 T2068 T2069 auxpass been,demonstrated
R1762 T1995 T1991 cc and,generated
R1763 T2069 T2069 ROOT demonstrated,demonstrated
R1764 T2070 T2071 aux to,be
R1765 T1996 T1997 advmod thus,are
R1766 T2071 T2069 xcomp be,demonstrated
R1767 T2072 T2071 acomp important,be
R1768 T2073 T2071 prep for,be
R1769 T1997 T1985 conj are,are
R1770 T2074 T2076 det the,induction
R1771 T2075 T2076 amod persistent,induction
R1772 T2076 T2073 pobj induction,for
R1773 T2077 T2076 prep of,induction
R1774 T1998 T1999 amod potential,targets
R1775 T2078 T2079 det these,cells
R1776 T2079 T2077 pobj cells,of
R1777 T2080 T2076 prep in,induction
R1778 T1999 T1997 attr targets,are
R1779 T2081 T2080 pobj vitro,in
R1780 T2082 T2080 cc and,in
R1781 T2000 T1999 prep for,targets
R1782 T2083 T2080 conj in,in
R1783 T2084 T2083 pobj vivo,in
R1784 T2085 T2069 punct ",",demonstrated
R1785 T2001 T2002 amod therapeutic,interventions
R1786 T2086 T2094 mark since,have
R1787 T2087 T2094 nsubj animals,have
R1788 T2088 T2087 acl lacking,animals
R1789 T2002 T2000 pobj interventions,for
R1790 T2089 T2090 det the,TGF-βRII
R1791 T2090 T2088 dobj TGF-βRII,lacking
R1792 T2091 T2090 prep on,TGF-βRII
R1793 T2092 T2093 compound T,cells
R1794 T2093 T2091 pobj cells,on
R1795 T2094 T2069 advcl have,demonstrated
R1796 T2003 T1985 punct .,are
R1797 T2095 T2098 amod fewer,cells
R1798 T2096 T2097 advmod peripherally,iTreg
R1799 T2097 T2098 amod iTreg,cells
R1800 T2004 T2006 det These,Treg
R1801 T2098 T2094 dobj cells,have
R1802 T2099 T2099 ROOT [,[
R1803 T2100 T2101 nummod 15,]
R1804 T2005 T2006 amod induced,Treg
R1805 T2101 T2099 dobj ],[
R1806 T2102 T2094 cc and,have
R1807 T2006 T2012 nsubjpass Treg,reported
R1808 T2007 T2006 punct (,Treg
R1809 T2103 T2094 conj suffer,have
R1810 T2104 T2103 prep from,suffer
R1811 T2008 T2006 appos iTreg,Treg
R1812 T2105 T2107 det a,cell
R1813 T2106 T2107 compound T,cell
R1814 T2107 T2104 pobj cell,from
R1815 T2009 T2006 punct ),Treg
R1816 T2108 T2111 amod dependent,disease
R1817 T2109 T2111 amod multiorgan,disease
R1818 T2010 T2012 nsubjpass cells,reported
R1819 T2110 T2111 amod inflammatory,disease
R1820 T2111 T2114 nmod disease,]
R1821 T2112 T2111 appos [,disease
R1822 T2011 T2012 auxpass were,reported
R1823 T2113 T2114 nummod 16,]
R1824 T2114 T2069 npadvmod ],demonstrated
R1825 T2115 T2069 punct .,demonstrated
R1826 T2116 T2130 mark Although,demonstrated
R1827 T2012 T2012 ROOT reported,reported
R1828 T2117 T2118 det the,effect
R1829 T2118 T2130 nsubjpass effect,demonstrated
R1830 T2119 T2118 prep of,effect
R1831 T2013 T2014 aux to,express
R1832 T2120 T2119 pobj TGF-β,of
R1833 T2121 T2118 prep on,effect
R1834 T2122 T2127 amod natural,induction
R1835 T2014 T2012 xcomp express,reported
R1836 T2123 T2122 cc and,natural
R1837 T2124 T2122 conj inducible,natural
R1838 T2015 T2018 nummod FOXP3,]
R1839 T2125 T2127 compound Treg,induction
R1840 T2126 T2127 compound cell,induction
R1841 T2127 T2121 pobj induction,on
R1842 T2128 T2130 aux has,demonstrated
R1843 T2129 T2130 auxpass been,demonstrated
R1844 T2130 T2139 advcl demonstrated,remain
R1845 T2016 T2018 nmod [,]
R1846 T2131 T2130 advmod repeatedly,demonstrated
R1847 T2132 T2134 compound [,]
R1848 T2133 T2134 compound "15,17",]
R1849 T2017 T2018 nummod 10,]
R1850 T2134 T2130 dobj ],demonstrated
R1851 T2135 T2139 punct ",",remain
R1852 T2136 T2138 poss its,mechanisms
R1853 T2018 T2014 dobj ],express
R1854 T2137 T2138 amod molecular,mechanisms
R1855 T2138 T2139 nsubj mechanisms,remain
R1856 T2019 T2012 punct .,reported
R1857 T2020 T2022 det The,mechanisms
R1858 T2021 T2022 amod exact,mechanisms
R1859 T2139 T2139 ROOT remain,remain
R1860 T2022 T2026 nsubj mechanisms,are
R1861 T2140 T2142 aux to,identified
R1862 T2141 T2142 auxpass be,identified
R1863 T2142 T2139 xcomp identified,remain
R1864 T2023 T2022 prep of,mechanisms
R1865 T2143 T2139 punct .,remain
R1866 T2144 T2146 det The,study
R1867 T2145 T2146 amod current,study
R1868 T2024 T2025 compound iTreg,generation
R1869 T2146 T2147 nsubj study,provides
R1870 T2147 T2147 ROOT provides,provides
R1871 T2025 T2023 pobj generation,of
R1872 T2148 T2147 dobj evidence,provides
R1873 T2149 T2153 mark that,play
R1874 T2150 T2153 nsubj GATA3,play
R1875 T2151 T2150 cc and,GATA3
R1876 T2026 T2026 ROOT are,are
R1877 T2152 T2150 conj FOXP3,GATA3
R1878 T2153 T2148 acl play,evidence
R1879 T2027 T2026 acomp unclear,are
R1880 T2154 T2156 det a,role
R1881 T2155 T2156 amod competitive,role
R1882 T2028 T2026 punct ",",are
R1883 T2156 T2153 dobj role,play
R1884 T2157 T2156 prep in,role
R1885 T2158 T2160 compound iTreg,commitment
R1886 T2029 T2026 cc but,are
R1887 T2159 T2160 compound cell,commitment
R1888 T2160 T2157 pobj commitment,in
R1889 T2161 T2160 prep as,commitment
R1890 T2162 T2161 pobj T-bet,as
R1891 T2163 T2162 cc and,T-bet
R1892 T2164 T2162 conj GATA3,T-bet
R1893 T2165 T2162 prep for,T-bet
R1894 T2166 T2165 pobj Th1,for
R1895 T2030 T2032 compound T,receptor
R1896 T2167 T2166 cc and,Th1
R1897 T2168 T2166 conj Th2,Th1
R1898 T2169 T2160 conj differentiation,commitment
R1899 T2031 T2032 compound cell,receptor
R1900 T2170 T2153 punct ",",play
R1901 T2171 T2153 advmod respectively,play
R1902 T2032 T2039 nsubjpass receptor,shown
R1903 T2172 T2147 punct .,provides
R1904 T2173 T2174 nsubj We,show
R1905 T2174 T2174 ROOT show,show
R1906 T2033 T2034 punct (,TCR
R1907 T2175 T2177 mark that,inhibits
R1908 T2176 T2177 nsubj GATA3,inhibits
R1909 T2177 T2174 ccomp inhibits,show
R1910 T2034 T2032 appos TCR,receptor
R1911 T2178 T2179 nummod FOXP3,induction
R1912 T2179 T2177 dobj induction,inhibits
R1913 T2180 T2177 cc and,inhibits
R1914 T2035 T2032 punct ),receptor
R1915 T2181 T2183 mark that,limits
R1916 T2036 T2039 nsubjpass triggering,shown
R1917 T2037 T2039 aux has,shown
R1918 T2182 T2183 nsubj IL-4,limits
R1919 T2183 T2177 conj limits,inhibits
R1920 T2038 T2039 auxpass been,shown
R1921 T2184 T2185 nummod FOXP3,expression
R1922 T2039 T2026 conj shown,are
R1923 T2040 T2041 aux to,induce
R1924 T2185 T2183 dobj expression,limits
R1925 T2041 T2039 xcomp induce,shown
R1927 T2186 T2183 prep in,limits
R1928 T2187 T2189 det a,way
R1929 T2188 T2189 amod GATA3-mediated,way
R1930 T2189 T2186 pobj way,in
R1931 T2190 T2183 punct ",",limits
R1932 T2191 T2192 preconj both,in
R1933 T2192 T2183 prep in,limits
R1934 T2193 T2192 pobj vitro,in
R1935 T2194 T2192 cc and,in
R1937 T2195 T2192 conj in,in
R1938 T2196 T2195 pobj vivo,in
R1940 T2197 T2174 punct .,show
R1941 T2198 T2200 nsubj We,show
R1942 T2199 T2200 advmod also,show
R1943 T2200 T2200 ROOT show,show
R1944 T2201 T2204 mark that,binds
R1945 T2202 T2204 nsubj GATA3,binds
R1946 T2203 T2204 advmod directly,binds
R1947 T2204 T2200 ccomp binds,show
R1949 T2205 T2204 prep to,binds
R1950 T2206 T2208 det the,promoter
R1951 T2207 T2208 nummod FOXP3,promoter
R1952 T2208 T2205 pobj promoter,to
R1953 T2209 T2204 cc and,binds
R1955 T2210 T2211 advmod thereby,prevents
R1956 T2211 T2204 conj prevents,binds
R1957 T2212 T2213 det the,induction
R1959 T2213 T2211 dobj induction,prevents
R1960 T2214 T2213 prep of,induction
R1961 T2215 T2216 det this,gene
R1962 T2216 T2214 pobj gene,of
R1964 T2217 T2200 punct ",",show
R1966 T2218 T2200 advcl demonstrating,show
R1967 T2219 T2222 mark that,overrules
R1969 T2220 T2221 amod Th2,differentiation
R1970 T2221 T2222 nsubj differentiation,overrules
R1971 T2222 T2218 ccomp overrules,demonstrating
R1972 T2223 T2224 compound iTreg,induction
R1973 T2224 T2222 dobj induction,overrules
R1975 T2225 T2200 punct .,show
R2960 T3688 T3689 nummod FOXP3,Induction
R2961 T3689 T3696 npadvmod Induction,assumed
R2962 T3690 T3689 prep in,Induction
R2963 T3691 T3693 compound T,Subsets
R2964 T3692 T3693 compound Cell,Subsets
R2965 T3693 T3690 pobj Subsets,in
R2966 T3694 T3696 nsubjpass It,assumed
R2967 T3695 T3696 auxpass is,assumed
R2968 T3696 T3696 ROOT assumed,assumed
R2969 T3697 T3702 mark that,induced
R2970 T3698 T3699 nummod FOXP3,expression
R2971 T3699 T3702 nsubjpass expression,induced
R2972 T3700 T3702 aux can,induced
R2973 T3701 T3702 auxpass be,induced
R2974 T3702 T3696 ccomp induced,assumed
R2975 T3703 T3702 prep in,induced
R2976 T3704 T3706 amod nonregulatory,cells
R2977 T3705 T3706 compound T,cells
R2978 T3706 T3703 pobj cells,in
R2979 T3707 T3706 punct ",",cells
R2980 T3708 T3709 nsubj which,is
R2981 T3709 T3702 advcl is,induced
R2982 T3710 T3712 det an,step
R2983 T3711 T3712 amod important,step
R2984 T3712 T3709 attr step,is
R2985 T3713 T3712 prep in,step
R2986 T3714 T3716 compound iTreg,differentiation
R2987 T3715 T3716 compound cell,differentiation
R2988 T3716 T3713 pobj differentiation,in
R2989 T3717 T3696 punct .,assumed
R2990 T3718 T3723 advmod However,known
R2991 T3719 T3723 punct ",",known
R2992 T3720 T3723 nsubjpass it,known
R2993 T3721 T3723 auxpass is,known
R2994 T3722 T3723 neg not,known
R2995 T3723 T3723 ROOT known,known
R2996 T3724 T3730 mark if,have
R2997 T3725 T3729 det all,cells
R2998 T3726 T3729 nummod CD4,cells
R2999 T3727 T3729 compound +,cells
R3000 T3728 T3729 compound T,cells
R3001 T3729 T3730 nsubj cells,have
R3002 T3730 T3723 advcl have,known
R3003 T3731 T3733 det the,capacity
R3004 T3732 T3733 amod same,capacity
R3005 T3733 T3730 dobj capacity,have
R3006 T3734 T3735 aux to,express
R3007 T3735 T3733 acl express,capacity
R3008 T3736 T3735 dobj FOXP3,express
R3009 T3737 T3723 punct .,known
R3010 T3738 T3739 aux To,investigate
R3011 T3739 T3764 advcl investigate,analyzed
R3012 T3740 T3744 mark whether,expressed
R3013 T3741 T3744 nsubjpass FOXP3,expressed
R3014 T3742 T3744 aux can,expressed
R3015 T3743 T3744 auxpass be,expressed
R3016 T3744 T3739 ccomp expressed,investigate
R3017 T3745 T3744 agent by,expressed
R3018 T3746 T3749 det any,subset
R3019 T3747 T3748 compound T,cell
R3020 T3748 T3749 compound cell,subset
R3021 T3749 T3745 pobj subset,by
R3022 T3750 T3744 cc or,expressed
R3023 T3751 T3754 mark if,restricted
R3024 T3752 T3754 nsubjpass expression,restricted
R3025 T3753 T3754 auxpass is,restricted
R3026 T3754 T3744 conj restricted,expressed
R3027 T3755 T3754 prep to,restricted
R3028 T3756 T3758 det a,lineage
R3029 T3757 T3758 amod distinct,lineage
R3030 T3758 T3755 pobj lineage,to
R3031 T3759 T3764 punct ",",analyzed
R3032 T3760 T3762 nummod FOXP3,expression
R3033 T3761 T3762 compound mRNA,expression
R3034 T3762 T3764 nsubjpass expression,analyzed
R3035 T3763 T3764 auxpass was,analyzed
R3036 T3764 T3811 ccomp analyzed,phenotype
R3037 T3765 T3764 prep in,analyzed
R3038 T3766 T3767 advmod freshly,isolated
R3039 T3767 T3769 amod isolated,cells
R3040 T3768 T3769 compound T,cells
R3041 T3769 T3765 pobj cells,in
R3042 T3770 T3771 amod such,as
R3043 T3771 T3769 prep as,cells
R3044 T3772 T3775 amod CD25-depleted,cells
R3045 T3773 T3775 compound CD4,cells
R3046 T3774 T3775 compound +,cells
R3047 T3775 T3771 pobj cells,as
R3048 T3776 T3764 punct ",",analyzed
R3049 T3777 T3778 compound CD45RA,+
R3050 T3778 T3779 nsubj +,naive
R3051 T3779 T3795 amod naive,cells
R3052 T3780 T3779 cc or,naive
R3053 T3781 T3785 nummod CD45RO,cells
R3054 T3782 T3785 amod +,cells
R3055 T3783 T3785 compound memory,cells
R3056 T3784 T3785 compound T,cells
R3057 T3785 T3779 conj cells,naive
R3058 T3786 T3787 punct (,Figure
R3059 T3787 T3785 appos Figure,cells
R3060 T3788 T3787 nummod 1A,Figure
R3061 T3789 T3787 punct ),Figure
R3062 T3790 T3787 punct ",",Figure
R3063 T3791 T3793 advmod as,as
R3064 T3792 T3793 advmod well,as
R3065 T3793 T3787 cc as,Figure
R3066 T3794 T3795 compound T,cells
R3067 T3795 T3811 dep cells,phenotype
R3068 T3796 T3795 acl driven,cells
R3069 T3797 T3796 prep in,driven
R3070 T3798 T3797 pobj vitro,in
R3071 T3799 T3798 prep toward,vitro
R3072 T3800 T3799 pobj Th1,toward
R3073 T3801 T3800 punct ",",Th1
R3074 T3802 T3800 conj Th2,Th1
R3075 T3803 T3802 punct ",",Th2
R3076 T3804 T3802 cc or,Th2
R3077 T3805 T3802 conj iTreg,Th2
R3078 T3806 T3802 conj phenotypes,Th2
R3079 T3807 T3808 punct (,Figure
R3080 T3808 T3795 appos Figure,cells
R3081 T3809 T3808 nummod 1B,Figure
R3082 T3810 T3811 punct ;,phenotype
R3083 T3811 T3811 ROOT phenotype,phenotype
R3084 T3812 T3811 prep on,phenotype
R3085 T3813 T3812 pobj Figure,on
R3086 T3814 T3813 nummod S1,Figure
R3087 T3815 T3811 punct ),phenotype
R3088 T3816 T3811 punct .,phenotype
R3089 T3817 T3820 det The,CD25
R3090 T3818 T3820 compound CD4,CD25
R3091 T3819 T3820 compound +,CD25
R3092 T3820 T3834 nsubj CD25,were
R3093 T3821 T3820 punct ",",CD25
R3094 T3822 T3823 compound CD45RA,+
R3095 T3823 T3820 appos +,CD25
R3096 T3824 T3823 punct ",",+
R3097 T3825 T3826 compound CD45RO,+
R3098 T3826 T3820 appos +,CD25
R3099 T3827 T3826 punct ",",+
R3100 T3828 T3826 cc and,+
R3101 T3829 T3833 compound CD4,CD25
R3102 T3830 T3833 compound +,CD25
R3103 T3831 T3833 compound CD45RO,CD25
R3104 T3832 T3833 compound +,CD25
R3105 T3833 T3826 conj CD25,+
R3106 T3834 T3834 ROOT were,were
R3107 T3835 T3834 acomp able,were
R3108 T3836 T3838 aux to,induce
R3109 T3837 T3838 advmod significantly,induce
R3110 T3838 T3835 xcomp induce,able
R3111 T3839 T3840 nummod FOXP3,mRNA
R3112 T3840 T3838 dobj mRNA,induce
R3113 T3841 T3838 cc up,induce
R3114 T3842 T3843 aux to,30-fold
R3115 T3843 T3838 xcomp 30-fold,induce
R3116 T3844 T3843 prep upon,30-fold
R3117 T3845 T3846 compound TCR,activation
R3118 T3846 T3844 pobj activation,upon
R3119 T3847 T3846 cc and,activation
R3120 T3848 T3846 conj addition,activation
R3121 T3849 T3848 prep of,addition
R3122 T3850 T3849 pobj TGF-β,of
R3123 T3851 T3834 punct .,were
R3124 T3852 T3853 nummod Th1,cells
R3125 T3853 T3854 nsubj cells,showed
R3126 T3854 T3854 ROOT showed,showed
R3127 T3855 T3858 advmod only,increase
R3128 T3856 T3858 det a,increase
R3129 T3858 T3854 dobj increase,showed
R3130 T3857 T3858 amod 10-fold,increase
R3131 T3859 T3854 punct .,showed
R3132 T3860 T3872 prep In,increase
R3133 T3898 T3895 pobj conditions,under
R3134 T3861 T3860 pobj contrast,In
R3135 T3862 T3872 punct ",",increase
R3136 T3863 T3864 amod Th2,cells
R3137 T3899 T3901 punct (,1B
R3138 T3864 T3872 nsubj cells,increase
R3139 T3865 T3864 acl stimulated,cells
R3140 T3866 T3865 prep under,stimulated
R3141 T3900 T3901 compound Figure,1B
R3142 T3867 T3869 det the,conditions
R3143 T3868 T3869 amod same,conditions
R3144 T3901 T3898 appos 1B,conditions
R3145 T3869 T3866 pobj conditions,under
R3146 T3870 T3872 aux did,increase
R3147 T3871 T3872 neg not,increase
R3148 T3872 T3872 ROOT increase,increase
R3149 T3902 T3904 punct ",",panel
R3150 T3873 T3874 nummod FOXP3,expression
R3151 T3874 T3872 dobj expression,increase
R3152 T3903 T3904 amod right,panel
R3153 T3875 T3872 punct .,increase
R3154 T3876 T3881 det The,cells
R3155 T3877 T3879 prep in,generated
R3156 T3904 T3901 appos panel,1B
R3157 T3878 T3877 pobj vitro,in
R3158 T3879 T3881 amod generated,cells
R3159 T3880 T3881 compound iTreg,cells
R3160 T3905 T3901 punct ),1B
R3161 T3881 T3882 nsubj cells,were
R3162 T3882 T3882 ROOT were,were
R3163 T3906 T3882 punct .,were
R3164 T3907 T3908 nummod Th2,Cells
R3165 T3883 T3882 acomp unable,were
R3166 T3884 T3885 aux to,further
R3167 T3885 T3883 xcomp further,unable
R3168 T3886 T3887 compound up-regulate,FOXP3
R3169 T3908 T3910 nsubjpass Cells,known
R3170 T3887 T3885 dobj FOXP3,further
R3171 T3888 T3887 punct ",",FOXP3
R3172 T3889 T3890 nsubj which,was
R3173 T3890 T3887 relcl was,FOXP3
R3174 T3909 T3910 auxpass are,known
R3175 T3891 T3890 advmod already,was
R3176 T3892 T3890 prep at,was
R3177 T3910 T3910 ROOT known,known
R3178 T3893 T3894 amod high,levels
R3179 T3894 T3892 pobj levels,at
R3180 T3895 T3890 prep under,was
R3181 T3911 T3912 aux to,produce
R3182 T3896 T3898 det the,conditions
R3183 T3897 T3898 amod resting,conditions
R3184 T3912 T3910 xcomp produce,known
R3185 T3913 T3912 dobj IL-4,produce
R3186 T3914 T3912 prep upon,produce
R3187 T3915 T3914 pobj activation,upon
R3188 T3916 T3915 punct ",",activation
R3189 T3917 T3919 nsubj which,interact
R3190 T3918 T3919 aux may,interact
R3191 T3995 T3993 dobj expression,lack
R3192 T3919 T3915 relcl interact,activation
R3193 T3996 T3998 punct (,2A
R3194 T3997 T3998 nmod Figure,2A
R3195 T3920 T3919 prep with,interact
R3196 T3998 T3995 appos 2A,expression
R3197 T3999 T3998 punct ),2A
R3198 T4000 T3983 punct .,documented
R3199 T3921 T3922 amod TGF-β,signaling
R3200 T4001 T4003 advmod Only,cells
R3201 T4002 T4003 amod iTreg,cells
R3202 T3922 T3920 pobj signaling,with
R3203 T4003 T4004 nsubj cells,expressed
R3204 T4004 T4004 ROOT expressed,expressed
R3205 T4005 T4004 dobj FOXP3,expressed
R3206 T3923 T3922 cc and,signaling
R3207 T4006 T4004 prep in,expressed
R3208 T4007 T4008 compound resting,conditions
R3209 T4008 T4006 pobj conditions,in
R3210 T4009 T4004 punct .,expressed
R3211 T3924 T3925 advmod thus,prevent
R3212 T4010 T4013 advmod Interestingly,observed
R3213 T4011 T4013 punct ",",observed
R3214 T4012 T4013 nsubj we,observed
R3215 T3925 T3919 conj prevent,interact
R3216 T4013 T4013 ROOT observed,observed
R3217 T4014 T4018 mark that,express
R3218 T4015 T4017 amod resting,cells
R3219 T4016 T4017 compound iTreg,cells
R3220 T4017 T4018 nsubj cells,express
R3221 T3926 T3927 nummod FOXP3,induction
R3222 T4018 T4013 ccomp express,observed
R3223 T4019 T4018 dobj FOXP3,express
R3224 T4020 T4018 cc but,express
R3225 T3927 T3925 dobj induction,prevent
R3226 T4021 T4018 conj show,express
R3227 T4022 T4023 amod low,CD25
R3228 T3928 T3910 punct .,known
R3229 T4023 T4024 compound CD25,surface
R3230 T4024 T4025 compound surface,expression
R3231 T4025 T4021 dobj expression,show
R3232 T4026 T4013 punct .,observed
R3233 T3929 T3942 advmod However,rescue
R3234 T4027 T4028 amod Repeated,exposure
R3235 T4028 T4034 nsubj exposure,increase
R3236 T4029 T4028 prep to,exposure
R3237 T3930 T3942 punct ",",rescue
R3238 T4030 T4029 pobj TGF-β,to
R3239 T4031 T4034 aux did,increase
R3240 T4032 T4033 neg not,further
R3241 T3931 T3932 det the,neutralization
R3242 T4033 T4034 advmod further,increase
R3243 T4034 T4034 ROOT increase,increase
R3244 T4035 T4037 det the,expression
R3245 T3932 T3942 nsubj neutralization,rescue
R3246 T4036 T4037 nummod FOXP3,expression
R3247 T4037 T4034 dobj expression,increase
R3248 T4038 T4037 prep in,expression
R3249 T4039 T4041 det the,lineage
R3250 T3933 T3932 prep of,neutralization
R3251 T4040 T4041 compound iTreg,lineage
R3252 T3934 T3933 pobj IL-4,of
R3253 T3935 T3932 prep with,neutralization
R3254 T4041 T4038 pobj lineage,in
R3255 T3936 T3939 det a,antibody
R3256 T4042 T4034 cc but,increase
R3257 T3937 T3939 amod blocking,antibody
R3258 T4043 T4044 advmod transiently,induced
R3259 T4044 T4034 conj induced,increase
R3260 T4045 T4046 nummod FOXP3,expression
R3261 T3938 T3939 compound IL-4,antibody
R3262 T4046 T4044 dobj expression,induced
R3263 T4047 T4044 prep in,induced
R3264 T4048 T4049 amod Th1,cells
R3265 T3939 T3935 pobj antibody,with
R3266 T4049 T4047 pobj cells,in
R3267 T4050 T4044 punct .,induced
R3268 T4051 T4052 advmod Naturally,occurring
R3269 T4052 T4064 advcl occurring,isolated
R3270 T4053 T4054 amod Th2,cells
R3271 T3940 T3942 aux did,rescue
R3272 T4054 T4052 dobj cells,occurring
R3273 T4055 T4056 amod such,as
R3274 T4056 T4054 prep as,cells
R3275 T3941 T3942 neg not,rescue
R3276 T4057 T4060 compound CRTH2,cells
R3277 T4058 T4060 compound +,cells
R3278 T4059 T4060 compound T,cells
R3279 T3942 T3942 ROOT rescue,rescue
R3280 T4060 T4056 pobj cells,as
R3281 T3943 T3944 nummod FOXP3,expression
R3282 T4061 T4064 punct ",",isolated
R3283 T4062 T4063 compound T,cells
R3284 T4063 T4064 nsubj cells,isolated
R3285 T4064 T4064 ROOT isolated,isolated
R3286 T3944 T3942 dobj expression,rescue
R3287 T4065 T4064 prep according,isolated
R3288 T4066 T4065 prep to,according
R3289 T4067 T4069 poss their,secretion
R3290 T3945 T3944 prep in,expression
R3291 T3946 T3949 det the,cells
R3292 T3947 T3949 amod differentiated,cells
R3293 T4068 T4069 compound IL-4,secretion
R3294 T3948 T3949 amod Th2,cells
R3295 T4069 T4066 pobj secretion,to
R3296 T3949 T3945 pobj cells,in
R3297 T4070 T4069 punct ",",secretion
R3298 T4071 T4069 cc or,secretion
R3299 T4072 T4073 det an,IL-4
R3300 T3950 T3952 punct (,data
R3301 T4073 T4069 conj IL-4,secretion
R3302 T4074 T4065 conj producing,according
R3303 T4075 T4077 compound T,clone
R3304 T3951 T3952 amod unpublished,data
R3305 T4076 T4077 compound cell,clone
R3306 T4077 T4074 dobj clone,producing
R3307 T4078 T4077 punct (,clone
R3308 T3952 T3949 appos data,cells
R3309 T4079 T4077 appos BR8,clone
R3310 T4080 T4077 punct ),clone
R3311 T4081 T4083 aux were,lacking
R3312 T3953 T3949 punct ),cells
R3313 T4082 T4083 advmod also,lacking
R3314 T4083 T4064 ccomp lacking,isolated
R3315 T4084 T4085 nummod FOXP3,expression
R3316 T4085 T4083 dobj expression,lacking
R3317 T4086 T4088 punct (,2B
R3318 T4087 T4088 compound Figure,2B
R3319 T4088 T4085 appos 2B,expression
R3320 T3954 T3942 punct .,rescue
R3321 T4089 T4064 punct ),isolated
R3322 T4090 T4064 punct .,isolated
R3323 T4091 T4092 advmod Furthermore,TGF-β
R3324 T3955 T3956 det These,data
R3325 T3956 T3957 nsubj data,demonstrated
R3326 T3957 T3957 ROOT demonstrated,demonstrated
R3327 T3958 T3961 mark that,have
R3328 T4092 T4093 nsubj TGF-β,mediated
R3329 T4093 T4093 ROOT mediated,mediated
R3330 T4094 T4095 nummod FOXP3,induction
R3331 T3959 T3960 amod Th2,cells
R3332 T4095 T4096 nsubj induction,failed
R3333 T4096 T4093 ccomp failed,mediated
R3334 T4097 T4096 prep in,failed
R3335 T3960 T3961 nsubj cells,have
R3336 T4098 T4099 det these,cells
R3337 T4099 T4097 pobj cells,in
R3338 T4100 T4096 prep in,failed
R3339 T3961 T3957 ccomp have,demonstrated
R3340 T4101 T4100 pobj contrast,in
R3341 T4102 T4101 prep to,contrast
R3342 T4103 T4106 det the,cells
R3343 T3962 T3964 det a,capacity
R3344 T4104 T4106 amod naive,cells
R3345 T4105 T4106 compound T,cells
R3346 T4106 T4102 pobj cells,to
R3347 T4107 T4109 punct (,2B
R3348 T4108 T4109 compound Figure,2B
R3349 T3963 T3964 amod limited,capacity
R3350 T4109 T4106 appos 2B,cells
R3351 T4110 T4106 punct ),cells
R3352 T4111 T4093 punct .,mediated
R3353 T3964 T3961 dobj capacity,have
R3354 T4112 T4114 mark Because,is
R3355 T4113 T4114 nsubj IL-4,is
R3356 T4114 T4134 advcl is,analyzed
R3357 T3965 T3966 aux to,express
R3358 T4115 T4118 det the,cytokine
R3359 T4116 T4118 amod key,cytokine
R3360 T4117 T4118 amod Th2,cytokine
R3361 T3966 T3964 acl express,capacity
R3362 T4118 T4114 attr cytokine,is
R3363 T3967 T3966 dobj FOXP3,express
R3364 T3968 T3970 punct (,1B
R3365 T4119 T4118 punct ",",cytokine
R3366 T3969 T3970 compound Figure,1B
R3367 T4120 T4121 det the,expression
R3368 T4121 T4134 nsubjpass expression,analyzed
R3369 T4122 T4121 prep of,expression
R3370 T4123 T4122 pobj IL-4,of
R3371 T4124 T4121 cc and,expression
R3372 T4125 T4121 conj FOXP3,expression
R3373 T3970 T3967 appos 1B,FOXP3
R3374 T4126 T4125 prep in,FOXP3
R3375 T4127 T4128 advmod freshly,isolated
R3376 T4128 T4129 amod isolated,CD4
R3377 T3971 T3970 punct ),1B
R3378 T4129 T4132 compound CD4,cells
R3379 T4130 T4132 nummod +,cells
R3380 T4131 T4132 compound T,cells
R3381 T3972 T3957 punct .,demonstrated
R3382 T4132 T4126 pobj cells,in
R3383 T4133 T4134 auxpass was,analyzed
R3384 T4134 T4134 ROOT analyzed,analyzed
R3385 T3973 T3974 det The,inability
R3386 T4135 T4134 agent by,analyzed
R3387 T4136 T4138 amod fluorescence,cell
R3388 T4137 T4138 amod activated,cell
R3389 T3974 T3983 nsubjpass inability,documented
R3390 T4138 T4135 pobj cell,by
R3391 T4139 T4138 acl sorting,cell
R3392 T4140 T4139 punct (,sorting
R3393 T3975 T3974 prep of,inability
R3394 T4141 T4139 appos FACS,sorting
R3395 T4142 T4134 punct ),analyzed
R3396 T4143 T4134 punct .,analyzed
R3397 T3976 T3977 amod Th2,cells
R3398 T4144 T4144 ROOT IL-4,IL-4
R3399 T4145 T4146 amod expressing,cells
R3400 T4146 T4147 nsubj cells,were
R3401 T3977 T3975 pobj cells,of
R3402 T4147 T4147 ROOT were,were
R3403 T4148 T4149 advmod most,abundant
R3404 T4149 T4147 acomp abundant,were
R3405 T4150 T4147 prep among,were
R3406 T3978 T3979 aux to,express
R3407 T4151 T4153 nummod CD45RO,CD25
R3408 T4152 T4153 nummod +,CD25
R3409 T3979 T3974 acl express,inability
R3410 T4153 T4150 pobj CD25,among
R3411 T4154 T4153 appos cells,CD25
R3412 T4155 T4153 punct ",",CD25
R3413 T4156 T4159 nsubj which,co-express
R3414 T3980 T3979 dobj FOXP3,express
R3415 T4157 T4159 aux did,co-express
R3416 T4158 T4159 neg not,co-express
R3417 T4159 T4153 relcl co-express,CD25
R3418 T4160 T4163 nmod FOXP3,3A
R3419 T4161 T4163 punct (,3A
R3420 T4162 T4163 compound Figure,3A
R3421 T3981 T3983 auxpass was,documented
R3422 T4163 T4159 oprd 3A,co-express
R3423 T4164 T4147 punct ",",were
R3424 T4165 T4147 dep left,were
R3425 T4166 T4165 dobj panel,left
R3426 T4167 T4147 punct ),were
R3427 T4168 T4147 punct .,were
R3428 T4169 T4178 prep In,expressed
R3429 T3982 T3983 advmod also,documented
R3430 T4170 T4169 pobj contrast,In
R3431 T4171 T4178 punct ",",expressed
R3432 T4172 T4174 compound CD45RO,CD25
R3433 T3983 T3983 ROOT documented,documented
R3434 T4173 T4174 compound +,CD25
R3435 T4174 T4178 nsubj CD25,expressed
R3436 T4175 T4176 amod +,cells
R3437 T3984 T3983 prep at,documented
R3438 T4176 T4178 nsubj cells,expressed
R3439 T4177 T4178 advmod abundantly,expressed
R3440 T4178 T4178 ROOT expressed,expressed
R3441 T3985 T3987 det the,level
R3442 T3986 T3987 amod single-cell,level
R3443 T3987 T3984 pobj level,at
R3444 T4179 T4178 dobj FOXP3,expressed
R3445 T3988 T3983 punct ",",documented
R3446 T4180 T4178 punct ",",expressed
R3447 T4181 T4182 mark while,lacking
R3448 T3989 T3983 advcl confirming,documented
R3449 T4182 T4178 advcl lacking,expressed
R3450 T4183 T4182 dobj IL-4,lacking
R3451 T3990 T3993 mark that,lack
R3452 T4184 T4186 punct (,3A
R3453 T4185 T4186 compound Figure,3A
R3454 T4186 T4183 parataxis 3A,IL-4
R3455 T4187 T4189 punct ",",panel
R3456 T4188 T4189 amod right,panel
R3457 T3991 T3992 amod Th2,cells
R3458 T3992 T3993 nsubj cells,lack
R3459 T3993 T3989 ccomp lack,confirming
R3460 T3994 T3995 nummod FOXP3,expression
R3461 T4189 T4186 appos panel,3A
R3462 T4190 T4186 punct ),3A
R3463 T4286 T4287 compound cell,isolation
R3464 T4191 T4178 punct .,expressed
R3465 T4192 T4193 mark As,shown
R3466 T4193 T4205 advcl shown,was
R3467 T4194 T4193 prep in,shown
R3468 T4195 T4196 compound Figure,3B
R3469 T4196 T4194 pobj 3B,in
R3470 T4287 T4288 compound isolation,technology
R3471 T4288 T4283 dobj technology,using
R3472 T4197 T4205 punct ",",was
R3473 T4198 T4199 det the,frequency
R3474 T4289 T4278 conj contained,enriched
R3475 T4199 T4205 nsubj frequency,was
R3476 T4200 T4199 prep of,frequency
R3477 T4201 T4204 det the,cells
R3478 T4290 T4292 det some,cells
R3479 T4202 T4204 compound IL-4,cells
R3480 T4203 T4204 compound +,cells
R3481 T4204 T4200 pobj cells,of
R3482 T4291 T4292 amod FOXP3-expressing,cells
R3483 T4205 T4205 ROOT was,was
R3484 T4206 T4207 advmod always,below
R3485 T4292 T4289 dobj cells,contained
R3486 T4207 T4205 prep below,was
R3487 T4208 T4209 nummod 1,%
R3488 T4209 T4207 pobj %,below
R3489 T4293 T4289 punct ",",contained
R3490 T4210 T4209 prep in,%
R3491 T4211 T4214 det the,cells
R3492 T4294 T4289 cc but,contained
R3493 T4212 T4214 nummod FOXP3,cells
R3494 T4213 T4214 compound +,cells
R3495 T4214 T4210 pobj cells,in
R3496 T4215 T4209 amod close,%
R3497 T4216 T4215 prep to,close
R3498 T4295 T4301 advmod importantly,overlap
R3499 T4217 T4218 det the,background
R3500 T4218 T4216 pobj background,to
R3501 T4296 T4301 punct ",",overlap
R3502 T4219 T4205 punct .,was
R3503 T4220 T4223 det The,cells
R3504 T4221 T4223 compound IL-4,cells
R3505 T4222 T4223 compound +,cells
R3506 T4297 T4298 det the,expression
R3507 T4223 T4225 nsubjpass cells,confined
R3508 T4224 T4225 auxpass were,confined
R3509 T4225 T4225 ROOT confined,confined
R3510 T4298 T4301 nsubj expression,overlap
R3511 T4226 T4225 prep to,confined
R3512 T4227 T4228 det the,FOXP3
R3513 T4228 T4226 pobj FOXP3,to
R3514 T4299 T4301 aux did,overlap
R3515 T4229 T4228 appos cells,FOXP3
R3516 T4230 T4225 punct ",",confined
R3517 T4231 T4232 mark as,shown
R3518 T4300 T4301 neg not,overlap
R3519 T4232 T4225 advcl shown,confined
R3520 T4233 T4232 prep for,shown
R3521 T4234 T4237 det the,CD25
R3522 T4235 T4237 compound CD45RO,CD25
R3523 T4236 T4237 compound +,CD25
R3524 T4237 T4233 pobj CD25,for
R3525 T4301 T4278 advcl overlap,enriched
R3526 T4238 T4232 punct ",",shown
R3527 T4239 T4242 compound CD45RO,+
R3528 T4240 T4242 compound +,+
R3529 T4241 T4242 compound CD25,+
R3530 T4302 T4278 punct .,enriched
R3531 T4242 T4242 ROOT +,+
R3532 T4243 T4242 punct ",",+
R3533 T4244 T4242 cc and,+
R3534 T4303 T4308 advcl Taken,indicate
R3535 T4245 T4247 compound CD45RO,CD25
R3536 T4246 T4247 compound +,CD25
R3537 T4247 T4242 conj CD25,+
R3538 T4304 T4303 advmod together,Taken
R3539 T4248 T4250 advmod +,cells
R3540 T4249 T4250 amod high,cells
R3541 T4305 T4308 punct ",",indicate
R3542 T4250 T4242 appos cells,+
R3543 T4251 T4253 punct (,3B
R3544 T4252 T4253 compound Figure,3B
R3545 T4253 T4250 appos 3B,cells
R3546 T4306 T4307 det these,data
R3547 T4254 T4250 punct ),cells
R3548 T4255 T4225 punct .,confined
R3549 T4256 T4270 prep In,expressed
R3550 T4307 T4308 nsubj data,indicate
R3551 T4257 T4256 pobj addition,In
R3552 T4258 T4270 punct ",",expressed
R3553 T4308 T4308 ROOT indicate,indicate
R3554 T4259 T4262 preconj neither,clone
R3555 T4260 T4262 det the,clone
R3556 T4261 T4262 amod Th2,clone
R3557 T4309 T4313 mark that,expressed
R3558 T4262 T4270 nsubj clone,expressed
R3559 T4263 T4262 punct (,clone
R3560 T4264 T4262 appos BR8,clone
R3561 T4310 T4313 nsubjpass FOXP3,expressed
R3562 T4265 T4262 punct ),clone
R3563 T4266 T4262 cc nor,clone
R3564 T4311 T4313 auxpass was,expressed
R3565 T4267 T4268 amod CRTH2,cells
R3566 T4268 T4262 conj cells,clone
R3567 T4269 T4270 advmod significantly,expressed
R3568 T4312 T4313 neg not,expressed
R3569 T4270 T4270 ROOT expressed,expressed
R3570 T4271 T4270 dobj FOXP3,expressed
R3571 T4272 T4274 punct (,3C
R3572 T4273 T4274 compound Figure,3C
R3573 T4313 T4308 ccomp expressed,indicate
R3574 T4274 T4271 appos 3C,FOXP3
R3575 T4314 T4313 agent by,expressed
R3576 T4275 T4274 punct ),3C
R3577 T4276 T4270 punct .,expressed
R3578 T4315 T4316 amod Th2,cells
R3579 T4277 T4278 nsubj Cells,enriched
R3580 T4278 T4278 ROOT enriched,enriched
R3581 T4316 T4314 pobj cells,by
R3582 T4279 T4278 prep for,enriched
R3583 T4280 T4282 poss their,secretion
R3584 T4281 T4282 compound IL-4,secretion
R3585 T4317 T4313 cc and,expressed
R3586 T4282 T4279 pobj secretion,for
R3587 T4283 T4278 advcl using,enriched
R3588 T4318 T4313 conj was,expressed
R3589 T4284 T4288 det the,technology
R3590 T4285 T4287 amod magnetic,isolation
R3591 T4319 T4318 neg not,was
R3592 T4320 T4318 acomp inducible,was
R3593 T4321 T4320 prep in,inducible
R3594 T4322 T4323 det those,cells
R3595 T4323 T4321 pobj cells,in
R3596 T4324 T4308 punct .,indicate
R4314 T5565 T5583 dep FOXP3,suggests
R4315 T5566 T5565 cc and,FOXP3
R4316 T5567 T5568 compound GATA3,Kinetics
R4317 T5568 T5571 nmod Kinetics,Cells
R4318 T5569 T5568 prep in,Kinetics
R4319 T5570 T5569 pcomp Differentiating,in
R4320 T5571 T5565 conj Cells,FOXP3
R4321 T5572 T5574 det The,capacity
R4322 T5573 T5574 amod limited,capacity
R4323 T5574 T5583 nsubj capacity,suggests
R4324 T5575 T5574 prep of,capacity
R4325 T5576 T5578 amod differentiated,cells
R4326 T5577 T5578 compound effector,cells
R4327 T5578 T5575 pobj cells,of
R4328 T5579 T5580 aux to,induce
R4329 T5580 T5574 acl induce,capacity
R4330 T5581 T5582 nummod FOXP3,expression
R4331 T5582 T5580 dobj expression,induce
R4332 T5583 T5583 ROOT suggests,suggests
R4333 T5584 T5587 mark that,has
R4334 T5585 T5586 compound iTreg,induction
R4335 T5586 T5587 nsubj induction,has
R4336 T5587 T5583 ccomp has,suggests
R4337 T5588 T5589 aux to,occur
R4338 T5589 T5587 xcomp occur,has
R4339 T5590 T5595 mark before,occurs
R4340 T5591 T5594 amod effector,differentiation
R4371 T5622 T5620 conj anti-IFN-γ,anti-IL-4
R4372 T5623 T5622 punct ",",anti-IFN-γ
R4373 T5624 T5622 cc and,anti-IFN-γ
R4374 T5625 T5626 amod anti,IL-12
R4375 T5626 T5616 appos IL-12,Th0
R4376 T5627 T5626 punct ),IL-12
R4377 T5628 T5616 punct ",",Th0
R4378 T5629 T5616 conj Th2,Th0
R4379 T5630 T5629 punct ",",Th2
R4380 T5631 T5629 cc and,Th2
R4381 T5632 T5633 compound iTreg,phenotypes
R4382 T5633 T5616 appos phenotypes,Th0
R4383 T5634 T5600 punct .,analyzed
R4384 T5635 T5645 prep After,showed
R4385 T5636 T5635 pobj initiation,After
R4386 T5637 T5636 prep of,initiation
R4387 T5638 T5640 det the,process
R4388 T5639 T5640 compound differentiation,process
R4389 T5640 T5637 pobj process,of
R4390 T5641 T5645 punct ",",showed
R4391 T5642 T5645 nsubj FOXP3,showed
R4392 T5643 T5642 cc and,FOXP3
R4393 T5644 T5642 conj GATA3,FOXP3
R4394 T5645 T5645 ROOT showed,showed
R4395 T5646 T5649 det a,kinetic
R4396 T5647 T5649 amod similar,kinetic
R4397 T5648 T5649 compound expression,kinetic
R4398 T5649 T5645 dobj kinetic,showed
R4399 T5650 T5645 prep within,showed
R4400 T5651 T5654 det the,d
R4401 T5652 T5654 amod first,d
R4402 T5653 T5654 nummod 3,d
R4403 T5654 T5650 pobj d,within
R4404 T5655 T5654 punct ",",d
R4405 T5656 T5658 nsubjpass which,considered
R4406 T5657 T5658 auxpass are,considered
R4407 T5658 T5654 relcl considered,d
R4408 T5659 T5660 aux to,be
R4409 T5660 T5658 xcomp be,considered
R4410 T5661 T5660 acomp critical,be
R4411 T5662 T5661 prep in,critical
R4412 T5663 T5665 compound T,commitment
R4413 T5664 T5665 compound cell,commitment
R4414 T5665 T5662 pobj commitment,in
R4415 T5666 T5668 quantmod [,]
R4416 T5667 T5668 nummod 18,]
R4417 T5668 T5654 appos ],d
R4418 T5669 T5645 punct .,showed
R4419 T5670 T5678 prep Under,increased
R4420 T5671 T5673 amod Th2,conditions
R4421 T5672 T5673 compound differentiation,conditions
R4422 T5673 T5670 pobj conditions,Under
R4423 T5674 T5678 punct ",",increased
R4424 T5675 T5677 compound FOXP3,expression
R4425 T5676 T5677 compound mRNA,expression
R4426 T5677 T5678 nsubj expression,increased
R4427 T5678 T5678 ROOT increased,increased
R4428 T5679 T5680 advmod only,marginally
R4429 T5680 T5678 advmod marginally,increased
R4430 T5681 T5683 punct (,4A
R4431 T5682 T5683 compound Figure,4A
R4432 T5683 T5678 npadvmod 4A,increased
R4433 T5684 T5678 punct ",",increased
R4434 T5685 T5678 conj left,increased
R4435 T5686 T5685 dobj panel,left
R4436 T5687 T5685 punct ),left
R4437 T5688 T5678 punct .,increased
R4438 T5689 T5701 advmod Thus,polarizes
R4439 T5690 T5695 punct ",",showed
R4440 T5691 T5695 mark although,showed
R4449 T5700 T5701 nsubj expression,polarizes
R4450 T5701 T5701 ROOT polarizes,polarizes
R4451 T5702 T5701 prep at,polarizes
R4452 T5703 T5704 det the,end
R4453 T5704 T5702 pobj end,at
R4454 T5705 T5704 prep of,end
R4455 T5706 T5708 det the,process
R4456 T5707 T5708 compound differentiation,process
R4457 T5708 T5705 pobj process,of
R4458 T5709 T5712 advmod when,cultured
R4459 T5710 T5712 nsubjpass cells,cultured
R4460 T5711 T5712 auxpass were,cultured
R4461 T5712 T5701 advcl cultured,polarizes
R4462 T5713 T5712 prep towards,cultured
R4463 T5714 T5713 pobj Th2,towards
R4464 T5715 T5714 cc or,Th2
R4465 T5716 T5714 conj iTreg,Th2
R4466 T5717 T5722 dep cells,4A
R4467 T5718 T5717 punct ",",cells
R4468 T5719 T5719 ROOT respectively,respectively
R4469 T5720 T5722 punct (,4A
R4470 T5721 T5722 compound Figure,4A
R4471 T5722 T5712 dep 4A,cultured
R4472 T5723 T5722 cc and,4A
R4473 T5724 T5722 conj 4B,4A
R4474 T5725 T5722 punct ),4A
R4475 T5726 T5701 punct .,polarizes
R4476 T5727 T5733 advmod Interestingly,expressing
R4477 T5728 T5733 punct ",",expressing
R4478 T5729 T5731 det the,cells
R4479 T5730 T5731 amod Th0,cells
R4480 T5731 T5733 nsubj cells,expressing
R4481 T5732 T5733 aux were,expressing
R4482 T5733 T5754 ccomp expressing,differed
R4483 T5734 T5735 advmod more,FOXP3
R4484 T5735 T5733 dobj FOXP3,expressing
R4485 T5736 T5735 prep than,FOXP3
R4486 T5737 T5739 det the,cells
R4487 T5738 T5739 amod Th2,cells
R4488 T5739 T5736 pobj cells,than
R4489 T5740 T5733 punct ",",expressing
R4490 T5741 T5733 cc but,expressing
R4491 T5742 T5733 conj expressed,expressing
R4492 T5743 T5744 amod low,levels
R4493 T5744 T5742 dobj levels,expressed
R4494 T5745 T5744 prep of,levels
R4495 T5746 T5745 pobj GATA3,of
R4496 T5747 T5754 punct ;,differed
R4497 T5748 T5754 advmod however,differed
R4498 T5749 T5754 punct ",",differed
R4499 T5750 T5752 det the,expression
R4500 T5751 T5752 compound protein,expression
R4501 T5752 T5754 nsubj expression,differed
R4502 T5753 T5754 advmod slightly,differed
R4503 T5754 T5754 ROOT differed,differed
R4504 T5755 T5754 prep from,differed
R4505 T5756 T5757 compound mRNA,expression
R4506 T5757 T5755 pobj expression,from
R4507 T5758 T5754 punct ",",differed
R4508 T5759 T5754 advcl suggesting,differed
R4509 T5760 T5762 advmod also,regulation
R4510 T5761 T5762 amod posttranslational,regulation
R4511 T5762 T5759 dobj regulation,suggesting
R4512 T5763 T5762 cc and,regulation
R4513 T5764 T5762 conj degradation,regulation
R4514 T5765 T5762 prep as,regulation
R4515 T5766 T5768 amod potential,mechanisms
R4516 T5767 T5768 amod additional,mechanisms
R4517 T5768 T5765 pobj mechanisms,as
R4518 T5769 T5768 prep in,mechanisms
R4519 T5770 T5772 det the,process
R4520 T5771 T5772 compound differentiation,process
R4521 T5772 T5769 pobj process,in
R4522 T5773 T5754 punct .,differed
R4523 T5774 T5775 det The,phenotype
R4524 T5775 T5779 nsubj phenotype,included
R4525 T5776 T5775 prep of,phenotype
R4526 T5777 T5778 compound iTreg,cells
R4527 T5778 T5776 pobj cells,of
R4528 T5779 T5779 ROOT included,included
R4529 T5780 T5782 det an,phenotype
R4530 T5781 T5782 amod anergic,phenotype
R4531 T5782 T5779 dobj phenotype,included
R4532 T5783 T5782 prep upon,phenotype
R4533 T5784 T5785 amod anti-CD3,re-stimulation
R4534 T5785 T5783 pobj re-stimulation,upon
R4535 T5786 T5787 punct (,Figure
R4536 T5787 T5782 appos Figure,phenotype
R4537 T5788 T5787 nummod S1A,Figure
R4538 T5789 T5787 punct ),Figure
R4539 T5790 T5787 punct ",",Figure
R4540 T5791 T5800 nmod CD103,expression
R4541 T5792 T5791 punct ",",CD103
R4542 T5793 T5791 conj CTLA-4,CD103
R4543 T5794 T5793 punct ",",CTLA-4
R4544 T5795 T5793 conj GITR,CTLA-4
R4545 T5796 T5795 punct ",",GITR
R4546 T5797 T5795 cc and,GITR
R4547 T5798 T5800 compound PD-1,expression
R4548 T5799 T5800 compound surface,expression
R4549 T5800 T5782 appos expression,phenotype
R4550 T5801 T5802 punct (,Figure
R4551 T5802 T5800 appos Figure,expression
R4552 T5803 T5802 nummod S1B,Figure
R4553 T5804 T5800 punct ),expression
R4554 T5805 T5779 punct .,included
R4555 T5806 T5814 prep On,seen
R4556 T5807 T5809 det the,level
R4557 T5808 T5809 amod single-cell,level
R4558 T5809 T5806 pobj level,On
R4559 T5810 T5814 punct ",",seen
R4560 T5811 T5814 nsubjpass it,seen
R4561 T5812 T5814 aux can,seen
R4562 T5813 T5814 auxpass be,seen
R4563 T5814 T5814 ROOT seen,seen
R4564 T5815 T5817 mark that,progress
R4565 T5816 T5817 nsubj cells,progress
R4566 T5817 T5814 ccomp progress,seen
R4567 T5818 T5817 prep through,progress
R4568 T5819 T5821 det a,phase
R4569 T5820 T5821 compound transition,phase
R4570 T5821 T5818 pobj phase,through
R4571 T5822 T5821 punct ",",phase
R4572 T5823 T5828 advmod where,coexist
R4573 T5824 T5828 nsubj GATA3,coexist
R4574 T5825 T5824 cc and,GATA3
R4575 T5826 T5824 conj FOXP3,GATA3
R4576 T5827 T5828 compound expression,coexist
R4577 T5828 T5821 relcl coexist,phase
R4578 T5829 T5828 prep to,coexist
R4579 T5830 T5831 det some,degree
R4580 T5831 T5829 pobj degree,to
R4581 T5832 T5831 prep in,degree
R4582 T5833 T5835 det the,cells
R4583 T5834 T5835 amod same,cells
R4584 T5835 T5832 pobj cells,in
R4585 T5836 T5835 punct ",",cells
R4586 T5837 T5839 nsubjpass which,resolved
R4587 T5838 T5839 auxpass is,resolved
R4588 T5839 T5835 relcl resolved,cells
R4589 T5840 T5839 prep in,resolved
R4590 T5841 T5842 compound iTreg,cells
R4591 T5842 T5840 pobj cells,in
R4592 T5843 T5839 prep after,resolved
R4593 T5844 T5845 nummod 7,d
R4594 T5845 T5843 pobj d,after
R4595 T5846 T5848 punct (,4B
R4596 T5847 T5848 compound Figure,4B
R4597 T5848 T5845 appos 4B,d
R4598 T5849 T5845 punct ),d
R4599 T5850 T5814 punct .,seen
R4600 T5851 T5856 advcl Taken,demonstrated
R4602 T5853 T5856 punct ",",demonstrated
R4603 T5854 T5855 det these,data
R4604 T5855 T5856 nsubj data,demonstrated
R4605 T5856 T5856 ROOT demonstrated,demonstrated
R4606 T5857 T5861 mark that,lost
R4607 T5858 T5859 amod Th2,cells
R4608 T5859 T5861 nsubj cells,lost
R4609 T5860 T5861 aux have,lost
R4610 T5861 T5856 ccomp lost,demonstrated
R4611 T5862 T5863 poss their,capacity
R4612 T5863 T5861 dobj capacity,lost
R4613 T5864 T5865 aux to,express
R4614 T5865 T5863 acl express,capacity
R4615 T5866 T5865 dobj FOXP3,express
R4616 T5867 T5861 cc and,lost
R4617 T5868 T5861 conj showed,lost
R4618 T5869 T5874 mark that,arise
R4619 T5870 T5874 nsubj Th2,arise
R4620 T5871 T5870 cc and,Th2
R4621 T5872 T5870 conj iTreg,Th2
R4622 T5873 T5874 nsubj cells,arise
R4623 T5874 T5868 ccomp arise,showed
R4624 T5875 T5874 prep from,arise
R4625 T5876 T5879 nummod two,pathways
R4626 T5877 T5879 amod different,pathways
R4627 T5878 T5879 compound differentiation,pathways
R4628 T5879 T5875 pobj pathways,from
R4629 T5880 T5856 punct .,demonstrated
R5399 T7041 T7042 nsubj IL-4,Inhibits
R5401 T7042 T7042 ROOT Inhibits,Inhibits
R5402 T7043 T7045 compound TGF-β-Mediated,Commitment
R5403 T7044 T7045 compound iTreg,Commitment
R5404 T7045 T7046 compound Commitment,IL-4
R5405 T7046 T7047 nsubj IL-4,induces
R5406 T7047 T7042 advcl induces,Inhibits
R5407 T7048 T7047 dobj differentiation,induces
R5408 T7049 T7048 prep of,differentiation
R5409 T7050 T7052 amod naive,cells
R5410 T7051 T7052 compound T,cells
R5411 T7052 T7049 pobj cells,of
R5412 T7053 T7047 punct ",",induces
R5413 T7054 T7047 prep upon,induces
R5414 T7055 T7056 compound antigen,encounter
R5415 T7056 T7054 pobj encounter,upon
R5416 T7057 T7047 punct ",",induces
R5417 T7058 T7047 prep into,induces
R5418 T7059 T7062 det the,lineage
R5419 T7060 T7062 compound Th2,lineage
R5420 T7079 T7077 pobj priming,during
R5421 T7061 T7062 compound cell,lineage
R5422 T7080 T7079 prep of,priming
R5423 T7081 T7083 amod naive,cells
R5424 T7082 T7083 compound T,cells
R5425 T7062 T7058 pobj lineage,into
R5426 T7083 T7080 pobj cells,of
R5427 T7084 T7066 punct .,asked
R5428 T7085 T7086 compound Human,CD4
R5429 T7063 T7042 punct .,Inhibits
R5430 T7086 T7093 nsubjpass CD4,activated
R5431 T7087 T7093 nsubjpass +,activated
R5432 T7088 T7089 nsubj CD45RA,+
R5433 T7089 T7093 advmod +,activated
R5434 T7064 T7066 nsubj We,asked
R5435 T7065 T7066 advmod therefore,asked
R5436 T7090 T7091 compound T,cells
R5437 T7066 T7066 ROOT asked,asked
R5438 T7067 T7069 mark whether,is
R5440 T7068 T7069 nsubj IL-4,is
R5443 T7094 T7093 prep with,activated
R5444 T7069 T7066 ccomp is,asked
R5445 T7095 T7097 nmod plate-bound,/
R5446 T7096 T7097 compound anti-CD3,/
R5447 T7097 T7094 pobj /,with
R5448 T7070 T7069 acomp able,is
R5449 T7098 T7097 nummod CD28,/
R5450 T7099 T7093 prep in,activated
R5451 T7100 T7101 det the,presence
R5452 T7071 T7072 aux to,inhibit
R5453 T7101 T7099 pobj presence,in
R5454 T7102 T7101 prep of,presence
R5455 T7072 T7070 xcomp inhibit,able
R5456 T7103 T7102 pobj TGF-β,of
R5457 T7104 T7101 cc and/or,presence
R5458 T7105 T7101 conj IL-4,presence
R5459 T7073 T7074 amod TGF-β,induction
R5460 T7106 T7105 cc and,IL-4
R5461 T7107 T7093 conj harvested,activated
R5462 T7108 T7113 mark after,repressed
R5463 T7074 T7072 dobj induction,inhibit
R5464 T7109 T7111 nummod 5,IL-4
R5465 T7110 T7111 compound d.,IL-4
R5466 T7111 T7113 nsubj IL-4,repressed
R5467 T7112 T7113 advmod efficiently,repressed
R5468 T7075 T7074 prep of,induction
R5469 T7113 T7107 advcl repressed,harvested
R5470 T7114 T7115 det the,TGF-β
R5471 T7115 T7113 dobj TGF-β,repressed
R5472 T7076 T7075 pobj FOXP3,of
R5473 T7116 T7093 conj mediated,activated
R5474 T7077 T7072 prep during,inhibit
R5475 T7117 T7116 dobj induction,mediated
R5476 T7118 T7117 prep of,induction
R5477 T7119 T7120 nummod FOXP3,expression
R5478 T7120 T7118 pobj expression,of
R5479 T7078 T7079 det the,priming
R5480 T7121 T7123 punct (,5A
R5481 T7179 T7180 det the,presence
R5482 T7122 T7123 nmod Figure,5A
R5483 T7123 T7120 appos 5A,expression
R5484 T7180 T7178 pobj presence,in
R5485 T7124 T7123 punct ),5A
R5486 T7125 T7116 prep in,mediated
R5487 T7126 T7128 det a,manner
R5488 T7127 T7128 amod concentration-dependent,manner
R5489 T7181 T7180 prep of,presence
R5490 T7128 T7125 pobj manner,in
R5491 T7129 T7131 punct (,5B
R5492 T7130 T7131 compound Figure,5B
R5493 T7131 T7128 appos 5B,manner
R5494 T7132 T7131 punct ),5B
R5495 T7133 T7116 punct .,mediated
R5496 T7182 T7181 pobj TGF-β,of
R5497 T7134 T7135 amod Low,levels
R5498 T7135 T7139 nsubjpass levels,induced
R5499 T7136 T7135 prep of,levels
R5500 T7137 T7136 pobj GATA3,of
R5501 T7183 T7180 prep at,presence
R5502 T7138 T7139 auxpass were,induced
R5503 T7139 T7139 ROOT induced,induced
R5504 T7140 T7139 advmod also,induced
R5505 T7184 T7186 amod high,concentration
R5506 T7141 T7139 prep in,induced
R5507 T7142 T7143 det the,absence
R5508 T7143 T7141 pobj absence,in
R5509 T7185 T7186 compound IL-4,concentration
R5510 T7144 T7143 prep of,absence
R5511 T7145 T7144 pobj IL-4,of
R5512 T7146 T7139 punct ",",induced
R5513 T7186 T7183 pobj concentration,at
R5514 T7147 T7151 mark as,observed
R5515 T7148 T7151 nsubjpass it,observed
R5516 T7149 T7151 auxpass was,observed
R5517 T7187 T7189 punct (,5A
R5518 T7150 T7151 advmod previously,observed
R5519 T7151 T7139 advcl observed,induced
R5520 T7152 T7154 nmod [,]
R5521 T7188 T7189 compound Figure,5A
R5522 T7153 T7154 nummod 3,]
R5523 T7154 T7151 dobj ],observed
R5524 T7189 T7186 appos 5A,concentration
R5525 T7155 T7139 punct .,induced
R5526 T7156 T7163 advmod However,was
R5527 T7157 T7163 punct ",",was
R5528 T7190 T7189 cc and,5A
R5529 T7158 T7163 prep at,was
R5530 T7159 T7160 amod low,concentration
R5531 T7160 T7158 pobj concentration,at
R5532 T7191 T7189 conj 5B,5A
R5533 T7161 T7163 punct ",",was
R5534 T7162 T7163 nsubj IL-4,was
R5535 T7163 T7163 ROOT was,was
R5536 T7164 T7163 acomp able,was
R5537 T7192 T7189 punct ),5A
R5538 T7165 T7167 aux to,induce
R5539 T7166 T7167 advmod marginally,induce
R5540 T7167 T7164 xcomp induce,able
R5541 T7193 T7177 punct .,induced
R5542 T7168 T7169 nummod FOXP3,expression
R5543 T7194 T7195 det The,IL-4
R5544 T7169 T7167 dobj expression,induce
R5545 T7170 T7163 punct .,was
R5546 T7171 T7177 prep Of,induced
R5547 T7195 T7203 nsubjpass IL-4,caused
R5548 T7172 T7171 pobj note,Of
R5549 T7196 T7203 nsubjpass mediated,caused
R5550 T7173 T7177 punct ",",induced
R5551 T7174 T7177 nsubjpass GATA3,induced
R5552 T7175 T7177 auxpass was,induced
R5553 T7197 T7196 dobj prevention,mediated
R5554 T7176 T7177 advmod also,induced
R5555 T7177 T7177 ROOT induced,induced
R5556 T7178 T7177 prep in,induced
R5557 T7198 T7197 prep of,prevention
R5558 T7199 T7200 nummod FOXP3,expression
R5559 T7200 T7198 pobj expression,of
R5560 T7201 T7203 auxpass was,caused
R5561 T7202 T7203 neg not,caused
R5562 T7203 T7203 ROOT caused,caused
R5563 T7276 T7275 pobj IL-4,of
R5564 T7277 T7274 prep into,injection
R5565 T7278 T7280 amod wild-type,mice
R5566 T7204 T7203 agent by,caused
R5567 T7279 T7280 compound B6,mice
R5568 T7280 T7277 pobj mice,into
R5569 T7205 T7204 pobj interferences,by
R5570 T7281 T7281 ROOT decreased,decreased
R5571 T7282 T7287 det the,number
R5572 T7283 T7287 amod inducible,number
R5573 T7206 T7205 prep of,interferences
R5574 T7284 T7283 cc or,inducible
R5575 T7285 T7283 conj natural,inducible
R5576 T7286 T7287 compound Treg,number
R5577 T7207 T7208 det the,receptor
R5578 T7287 T7281 dobj number,decreased
R5579 T7288 T7281 prep in,decreased
R5580 T7289 T7288 pobj vivo,in
R5581 T7208 T7206 pobj receptor,of
R5582 T7290 T7281 punct .,decreased
R5583 T7291 T7292 det A,distinction
R5584 T7209 T7205 acl signaling,interferences
R5585 T7292 T7297 nsubj distinction,is
R5586 T7293 T7292 prep of,distinction
R5587 T7294 T7296 det the,subsets
R5588 T7210 T7203 punct ",",caused
R5589 T7295 T7296 compound Treg,subsets
R5590 T7296 T7293 pobj subsets,of
R5591 T7297 T7297 ROOT is,is
R5592 T7211 T7220 mark because,affected
R5593 T7298 T7297 neg not,is
R5594 T7299 T7297 acomp possible,is
R5595 T7300 T7297 punct ",",is
R5596 T7301 T7307 mark because,express
R5597 T7302 T7303 advmod recently,activated
R5598 T7303 T7305 amod activated,cells
R5599 T7212 T7213 det the,phosphorylation
R5600 T7304 T7305 compound iTreg,cells
R5601 T7305 T7307 nsubj cells,express
R5602 T7306 T7307 advmod also,express
R5603 T7307 T7297 advcl express,is
R5604 T7213 T7220 nsubjpass phosphorylation,affected
R5605 T7308 T7309 compound surface,CD25
R5606 T7309 T7307 dobj CD25,express
R5607 T7310 T7297 punct .,is
R5608 T7214 T7213 prep of,phosphorylation
R5609 T7311 T7312 nsubj We,used
R5610 T7312 T7312 ROOT used,used
R5611 T7313 T7312 dobj complexes,used
R5612 T7215 T7214 pobj SMAD2,of
R5613 T7314 T7313 prep of,complexes
R5614 T7315 T7317 amod recombinant,IL-4
R5615 T7316 T7317 compound mouse,IL-4
R5616 T7317 T7314 pobj IL-4,of
R5617 T7216 T7215 cc or,SMAD2
R5618 T7318 T7319 punct (,rmIL-4
R5619 T7319 T7317 appos rmIL-4,IL-4
R5620 T7320 T7319 punct ),rmIL-4
R5621 T7217 T7215 conj STAT6,SMAD2
R5622 T7321 T7317 cc plus,IL-4
R5623 T7322 T7324 amod anti-IL-4,antibodies
R5624 T7323 T7324 amod monoclonal,antibodies
R5625 T7218 T7220 auxpass was,affected
R5626 T7324 T7317 conj antibodies,IL-4
R5627 T7219 T7220 neg not,affected
R5629 T7220 T7203 advcl affected,caused
R5632 T7221 T7220 agent by,affected
R5634 T7222 T7223 det the,addition
R5635 T7329 T7332 nsubjpass which,shown
R5636 T7223 T7221 pobj addition,by
R5637 T7330 T7332 aux have,shown
R5638 T7331 T7332 auxpass been,shown
R5639 T7332 T7324 relcl shown,antibodies
R5640 T7224 T7223 prep of,addition
R5641 T7333 T7335 aux to,increase
R5642 T7334 T7335 advmod dramatically,increase
R5643 T7335 T7332 xcomp increase,shown
R5644 T7225 T7224 pobj IL-4,of
R5645 T7336 T7337 det the,potency
R5646 T7337 T7335 dobj potency,increase
R5647 T7338 T7337 prep of,potency
R5648 T7339 T7340 det the,cytokine
R5649 T7340 T7338 pobj cytokine,of
R5650 T7226 T7225 cc and/or,IL-4
R5651 T7341 T7340 prep in,cytokine
R5652 T7342 T7343 compound vivo,[
R5653 T7343 T7341 pobj [,in
R5654 T7344 T7345 nummod 19,]
R5655 T7227 T7225 conj TGF-β,IL-4
R5656 T7345 T7343 appos ],[
R5657 T7346 T7312 punct .,used
R5658 T7228 T7225 punct ",",IL-4
R5659 T7347 T7364 prep In,decreased
R5660 T7348 T7349 det these,mice
R5661 T7349 T7347 pobj mice,In
R5662 T7350 T7364 punct ",",decreased
R5663 T7229 T7230 nsubj which,demonstrates
R5664 T7351 T7352 det the,percentage
R5665 T7352 T7364 nsubj percentage,decreased
R5666 T7353 T7352 prep of,percentage
R5667 T7230 T7223 relcl demonstrates,addition
R5668 T7354 T7357 compound CD4,+
R5669 T7355 T7357 compound +,+
R5670 T7356 T7357 compound CD25,+
R5671 T7231 T7238 mark that,were
R5672 T7357 T7352 conj +,percentage
R5673 T7358 T7357 cc and,+
R5674 T7359 T7362 nummod FOXP3,cells
R5675 T7232 T7238 nsubj IL-4,were
R5676 T7360 T7362 nummod +,cells
R5677 T7361 T7362 compound T,cells
R5678 T7362 T7364 nsubj cells,decreased
R5679 T7233 T7235 advmod as,as
R5680 T7363 T7364 advmod dramatically,decreased
R5681 T7364 T7364 ROOT decreased,decreased
R5682 T7365 T7371 advmod when,injected
R5683 T7234 T7235 advmod well,as
R5684 T7366 T7369 det the,complexes
R5685 T7367 T7369 amod antibody-cytokine,complexes
R5686 T7235 T7232 cc as,IL-4
R5687 T7368 T7369 amod immune,complexes
R5688 T7369 T7371 nsubjpass complexes,injected
R5689 T7370 T7371 auxpass were,injected
R5690 T7236 T7237 amod TGF-β,signaling
R5691 T7371 T7364 advcl injected,decreased
R5692 T7372 T7373 punct (,Figure
R5693 T7237 T7232 conj signaling,IL-4
R5694 T7238 T7230 ccomp were,demonstrates
R5695 T7373 T7371 dep Figure,injected
R5696 T7374 T7373 nummod S2,Figure
R5697 T7239 T7238 acomp functional,were
R5698 T7375 T7373 punct ),Figure
R5699 T7376 T7364 punct .,decreased
R5700 T7377 T7377 ROOT Upon,Upon
R5701 T7240 T7238 prep under,were
R5702 T7378 T7377 pobj administration,Upon
R5703 T7379 T7378 prep of,administration
R5704 T7380 T7384 nmod rmIL-4,complexes
R5705 T7241 T7242 det these,conditions
R5706 T7381 T7380 cc plus,rmIL-4
R5707 T7382 T7384 amod anti-IL-4,complexes
R5708 T7383 T7384 compound mAb,complexes
R5709 T7242 T7240 pobj conditions,under
R5710 T7384 T7379 pobj complexes,of
R5711 T7385 T7378 punct ",",administration
R5712 T7386 T7388 det the,number
R5713 T7387 T7388 amod total,number
R5714 T7243 T7245 punct (,5C
R5715 T7388 T7377 dobj number,Upon
R5716 T7389 T7388 prep of,number
R5717 T7390 T7395 nmod CD4,cell
R5718 T7244 T7245 compound Figure,5C
R5719 T7391 T7395 nmod +,cell
R5720 T7392 T7395 nmod CD25,cell
R5721 T7393 T7395 amod +,cell
R5722 T7245 T7242 appos 5C,conditions
R5723 T7394 T7395 compound T,cell
R5724 T7395 T7389 pobj cell,of
R5725 T7396 T7388 punct ",",number
R5726 T7246 T7220 punct ),affected
R5727 T7397 T7399 advmod as,as
R5728 T7398 T7399 advmod well,as
R5729 T7399 T7388 cc as,number
R5730 T7247 T7203 punct .,caused
R5731 T7248 T7249 amod Increasing,amounts
R5732 T7400 T7404 det the,cells
R5733 T7249 T7258 nsubj amounts,decreased
R5734 T7401 T7404 amod Foxp3,cells
R5735 T7402 T7404 amod +,cells
R5736 T7250 T7249 prep of,amounts
R5737 T7403 T7404 compound T,cells
R5738 T7404 T7388 conj cells,number
R5739 T7405 T7404 acl diminished,cells
R5740 T7251 T7252 compound IL-4,increase
R5741 T7406 T7405 agent by,diminished
R5742 T7252 T7249 appos increase,amounts
R5743 T7407 T7409 predet half,Figure
R5744 T7253 T7254 compound intracellular,GATA3
R5745 T7408 T7409 punct (,Figure
R5746 T7409 T7406 pobj Figure,by
R5747 T7410 T7409 nummod S2G,Figure
R5748 T7411 T7409 cc and,Figure
R5749 T7412 T7409 conj S2H,Figure
R5750 T7413 T7409 punct ),Figure
R5751 T7254 T7252 appos GATA3,increase
R5752 T7414 T7404 punct ",",cells
R5753 T7415 T7388 acl confirming,number
R5754 T7416 T7420 mark that,was
R5755 T7255 T7249 punct ",",amounts
R5756 T7417 T7419 det the,percentage
R5757 T7418 T7419 amod lower,percentage
R5758 T7419 T7420 nsubj percentage,was
R5759 T7256 T7258 mark whereas,decreased
R5760 T7420 T7415 ccomp was,confirming
R5761 T7421 T7420 neg not,was
R5762 T7422 T7420 acomp due,was
R5763 T7257 T7258 nsubj FOXP3,decreased
R5764 T7423 T7422 prep to,due
R5765 T7424 T7425 det an,increase
R5766 T7425 T7423 pobj increase,to
R5767 T7258 T7258 ROOT decreased,decreased
R5768 T7426 T7425 prep in,increase
R5769 T7427 T7430 det the,CD25
R5770 T7428 T7430 compound CD4,CD25
R5771 T7259 T7258 punct ",",decreased
R5772 T7429 T7430 compound +,CD25
R5773 T7430 T7426 pobj CD25,in
R5774 T7431 T7420 npadvmod cells,was
R5775 T7432 T7420 punct ",",was
R5776 T7260 T7261 nsubj which,is
R5777 T7433 T7420 cc but,was
R5778 T7434 T7436 det a,decrease
R5779 T7435 T7436 amod real,decrease
R5780 T7261 T7258 advcl is,decreased
R5781 T7436 T7420 conj decrease,was
R5782 T7437 T7436 prep of,decrease
R5783 T7438 T7440 compound CD4,CD25
R5784 T7439 T7440 compound +,CD25
R5785 T7262 T7261 acomp consistent,is
R5786 T7440 T7437 pobj CD25,of
R5787 T7441 T7443 amod +,cells
R5788 T7442 T7443 compound T,cells
R5789 T7263 T7262 prep with,consistent
R5790 T7443 T7436 conj cells,decrease
R5791 T7444 T7377 punct .,Upon
R5792 T7445 T7450 prep In,regulates
R5793 T7264 T7266 det the,analysis
R5794 T7446 T7445 pobj conclusion,In
R5795 T7447 T7450 punct ",",regulates
R5796 T7448 T7450 nsubj IL-4,regulates
R5797 T7449 T7450 advmod negatively,regulates
R5798 T7450 T7450 ROOT regulates,regulates
R5799 T7451 T7457 det the,turnover
R5800 T7452 T7457 amod natural,turnover
R5801 T7265 T7266 compound mRNA,analysis
R5802 T7453 T7452 cc or,natural
R5803 T7454 T7452 conj inducible,natural
R5804 T7455 T7457 compound Treg,turnover
R5805 T7266 T7263 pobj analysis,with
R5806 T7456 T7457 compound cell,turnover
R5807 T7457 T7450 dobj turnover,regulates
R5808 T7458 T7460 preconj not,in
R5809 T7459 T7458 advmod only,not
R5810 T7267 T7268 punct (,Figure
R5811 T7460 T7450 prep in,regulates
R5812 T7461 T7460 pobj vitro,in
R5813 T7462 T7460 cc but,in
R5814 T7268 T7266 appos Figure,analysis
R5815 T7463 T7462 advmod also,but
R5816 T7464 T7460 conj in,in
R5817 T7465 T7464 pobj vivo,in
R5818 T7269 T7268 nummod 5D,Figure
R5819 T7466 T7450 punct .,regulates
R5820 T7467 T7468 aux To,study
R5821 T7468 T7483 advcl study,exposed
R5822 T7270 T7268 punct ),Figure
R5823 T7469 T7470 det the,effects
R5824 T7271 T7258 punct .,decreased
R5825 T7272 T7281 advmod Furthermore,decreased
R5826 T7470 T7468 dobj effects,study
R5827 T7471 T7470 prep of,effects
R5828 T7273 T7281 punct ",",decreased
R5829 T7472 T7471 pobj IL-4,of
R5830 T7473 T7470 prep on,effects
R5831 T7474 T7480 amod already-existing,cells
R5832 T7274 T7281 nsubj injection,decreased
R5833 T7475 T7476 amod human,natural
R5834 T7476 T7480 amod natural,cells
R5835 T7477 T7476 cc or,natural
R5836 T7275 T7274 prep of,injection
R5837 T7478 T7476 conj inducible,natural
R5838 T7479 T7480 compound Treg,cells
R5839 T7480 T7473 pobj cells,on
R5841 T7481 T7483 punct ",",exposed
R5842 T7482 T7483 nsubj we,exposed
R5843 T7483 T7483 ROOT exposed,exposed
R5844 T7484 T7488 amod sorted,cells
R5845 T7485 T7488 nmod CD25,cells
R5846 T7486 T7488 nummod +,cells
R5847 T7487 T7488 compound T,cells
R5848 T7488 T7483 dobj cells,exposed
R5849 T7489 T7488 punct (,cells
R5850 T7490 T7491 amod nTreg,cells
R5851 T7491 T7488 appos cells,cells
R5852 T7492 T7488 punct ),cells
R5853 T7493 T7483 prep to,exposed
R5855 T7494 T7493 pobj IL-4,to
R5856 T7495 T7483 cc and,exposed
R5857 T7496 T7483 conj analyzed,exposed
R5858 T7497 T7498 nummod FOXP3,expression
R5859 T7498 T7496 dobj expression,analyzed
R5861 T7499 T7498 cc and,expression
R5862 T7500 T7501 amod suppressive,capacity
R5863 T7501 T7498 conj capacity,expression
R5865 T7502 T7483 punct .,exposed
R5866 T7503 T7509 prep In,failed
R5867 T7504 T7506 amod already-existing,cells
R5868 T7505 T7506 compound Treg,cells
R5870 T7506 T7503 pobj cells,In
R5871 T7507 T7509 punct ",",failed
R5872 T7508 T7509 nsubj IL-4,failed
R5873 T7509 T7509 ROOT failed,failed
R5874 T7510 T7511 aux to,inhibit
R5875 T7511 T7509 xcomp inhibit,failed
R5876 T7512 T7513 nummod FOXP3,expression
R5877 T7513 T7511 dobj expression,inhibit
R5878 T7514 T7515 punct (,Figure
R5879 T7515 T7513 appos Figure,expression
R5880 T7516 T7515 nummod S3A,Figure
R5882 T7517 T7515 punct ),Figure
R5883 T7518 T7509 punct ",",failed
R5884 T7519 T7509 cc and,failed
R5885 T7520 T7522 det the,capacity
R5886 T7521 T7522 amod suppressive,capacity
R5888 T7522 T7525 nsubjpass capacity,altered
R5889 T7523 T7525 auxpass was,altered
R5890 T7524 T7525 neg not,altered
R5891 T7525 T7509 conj altered,failed
R5893 T7526 T7525 punct (,altered
R5894 T7527 T7529 nmod Figure,)
R5895 T7528 T7527 nummod S3C,Figure
R5896 T7529 T7525 punct ),altered
R5897 T7530 T7525 punct .,altered
R5898 T7531 T7537 advmod Similarly,decrease
R5899 T7532 T7534 amod pre-existing,cells
R5901 T7533 T7534 compound iTreg,cells
R5902 T7534 T7537 nsubj cells,decrease
R5903 T7535 T7537 aux did,decrease
R5904 T7536 T7537 neg not,decrease
R5906 T7537 T7537 ROOT decrease,decrease
R5907 T7538 T7539 nummod FOXP3,expression
R5908 T7539 T7537 dobj expression,decrease
R5909 T7540 T7539 prep upon,expression
R5911 T7541 T7542 compound IL-4,exposure
R5912 T7542 T7540 pobj exposure,upon
R5913 T7543 T7544 punct (,Figure
R5914 T7544 T7542 appos Figure,exposure
R5915 T7545 T7544 nummod S3B,Figure
R5917 T7546 T7544 punct ),Figure
R5918 T7547 T7537 punct .,decrease
R6683 T8764 T8765 det The,Role
R6684 T8765 T8765 ROOT Role,Role
R6685 T8766 T8765 prep of,Role
R6686 T8767 T8766 pobj TGF-β,of
R6687 T8768 T8765 prep in,Role
R6688 T8769 T8771 compound T,Differentiation
R6689 T8770 T8771 compound Cell,Differentiation
R6690 T8771 T8768 pobj Differentiation,in
R6691 T8772 T8774 mark Although,reduced
R6692 T8773 T8774 nsubj TGF-β,reduced
R6693 T8774 T8790 advcl reduced,inhibited
R6694 T8775 T8774 dobj CD25,reduced
R6695 T8776 T8775 punct ",",CD25
R6696 T8777 T8778 compound IL-4,expression
R6697 T8778 T8775 appos expression,CD25
R6698 T8779 T8775 punct ",",CD25
R6699 T8780 T8775 cc and,CD25
R6700 T8781 T8782 compound CD25,expression
R6701 T8782 T8775 conj expression,CD25
R6702 T8783 T8785 punct (,6B
R6703 T8784 T8785 nmod Figure,6B
R6704 T8785 T8782 appos 6B,expression
R6705 T8786 T8785 punct ),6B
R6706 T8787 T8790 punct ",",inhibited
R6707 T8788 T8790 nsubj IL-4,inhibited
R6708 T8789 T8790 advmod significantly,inhibited
R6709 T8790 T8790 ROOT inhibited,inhibited
R6710 T8791 T8790 dobj TGF-β,inhibited
R6711 T8792 T8790 conj mediated,inhibited
R6712 T8793 T8792 dobj induction,mediated
R6713 T8794 T8793 prep of,induction
R6714 T8795 T8794 pobj FOXP3,of
R6715 T8796 T8795 prep in,FOXP3
R6716 T8797 T8799 amod naive,cells
R6717 T8798 T8799 compound T,cells
R6718 T8799 T8796 pobj cells,in
R6719 T8800 T8792 conj driven,mediated
R6720 T8801 T8800 prep toward,driven
R6721 T8802 T8805 nummod FOXP3,cells
R6722 T8803 T8805 nummod +,cells
R6723 T8804 T8805 compound T,cells
R6724 T8805 T8801 pobj cells,toward
R6725 T8806 T8800 punct ",",driven
R6726 T8807 T8808 mark as,shown
R6727 T8808 T8800 advcl shown,driven
R6728 T8809 T8808 agent by,shown
R6729 T8810 T8811 compound FACS,analysis
R6730 T8811 T8809 pobj analysis,by
R6731 T8812 T8814 punct (,6A
R6732 T8813 T8814 compound Figure,6A
R6733 T8814 T8811 appos 6A,analysis
R6734 T8815 T8800 punct ),driven
R6735 T8816 T8790 punct .,inhibited
R6736 T8817 T8819 nsubjpass It,known
R6737 T8818 T8819 auxpass is,known
R6738 T8819 T8819 ROOT known,known
R6739 T8820 T8822 mark that,is
R6740 T8821 T8822 nsubj IL-4,is
R6741 T8822 T8819 ccomp is,known
R6742 T8823 T8826 det a,factor
R6743 T8824 T8826 amod potent,factor
R6744 T8825 T8826 compound growth,factor
R6745 T8826 T8822 attr factor,is
R6746 T8827 T8822 cc and,is
R6747 T8828 T8830 aux may,favor
R6748 T8829 T8830 advmod therefore,favor
R6749 T8830 T8822 conj favor,is
R6750 T8831 T8832 det the,proliferation
R6751 T8832 T8830 dobj proliferation,favor
R6752 T8833 T8832 prep of,proliferation
R6753 T8834 T8833 pobj FOXP3,of
R6754 T8835 T8832 appos cells,proliferation
R6755 T8836 T8830 cc and,favor
R6756 T8837 T8838 advmod thus,decrease
R6757 T8838 T8830 conj decrease,favor
R6758 T8839 T8841 det the,percentage
R6759 T8840 T8841 amod relative,percentage
R6760 T8841 T8838 dobj percentage,decrease
R6761 T8842 T8841 prep of,percentage
R6762 T8843 T8845 nummod FOXP3,cells
R6763 T8844 T8845 nummod +,cells
R6764 T8845 T8842 pobj cells,of
R6765 T8846 T8819 punct .,known
R6766 T8847 T8856 advmod However,demonstrated
R6767 T8848 T8856 punct ",",demonstrated
R6768 T8849 T8856 nsubj analysis,demonstrated
R6769 T8850 T8849 prep of,analysis
R6770 T8851 T8852 compound cell,division
R6771 T8852 T8853 compound division,kinetics
R6772 T8853 T8850 pobj kinetics,of
R6773 T8854 T8849 prep by,analysis
R6774 T8855 T8854 pobj CFSE-labeling,by
R6775 T8856 T8856 ROOT demonstrated,demonstrated
R6776 T8857 T8862 mark that,promote
R6781 T8862 T8856 ccomp promote,demonstrated
R6782 T8863 T8864 compound cell,growth
R6783 T8864 T8862 dobj growth,promote
R6784 T8865 T8864 prep of,growth
R6785 T8866 T8867 nummod FOXP3,+
R6786 T8867 T8865 pobj +,of
R6787 T8868 T8864 prep over,growth
R6788 T8869 T8868 pobj that,over
R6789 T8870 T8869 prep of,that
R6790 T8871 T8870 pobj FOXP3,of
R6791 T8872 T8856 punct .,demonstrated
R6792 T8873 T8877 prep In,showed
R6793 T8874 T8873 pobj fact,In
R6794 T8875 T8876 det both,populations
R6795 T8876 T8877 nsubj populations,showed
R6796 T8877 T8877 ROOT showed,showed
R6797 T8878 T8879 advmod similarly,enhanced
R6798 T8879 T8880 amod enhanced,proliferation
R6799 T8880 T8877 dobj proliferation,showed
R6800 T8881 T8883 punct (,6A
R6801 T8882 T8883 compound Figure,6A
R6802 T8883 T8880 appos 6A,proliferation
R6803 T8884 T8880 punct ),proliferation
R6804 T8885 T8877 punct .,showed
R6805 T8886 T8889 advmod Furthermore,mediated
R6806 T8887 T8888 det the,TGF-β
R6807 T8888 T8889 nsubj TGF-β,mediated
R6808 T8889 T8889 ROOT mediated,mediated
R6809 T8890 T8889 dobj induction,mediated
R6810 T8891 T8890 prep of,induction
R6811 T8892 T8893 amod FOXP3,expression
R6812 T8893 T8891 pobj expression,of
R6813 T8894 T8896 auxpass was,caused
R6814 T8895 T8896 neg not,caused
R6815 T8896 T8889 conj caused,mediated
R6816 T8897 T8896 agent by,caused
R6817 T8898 T8897 pobj overgrowth,by
R6818 T8899 T8898 prep of,overgrowth
R6819 T8900 T8901 det a,CD25
R6820 T8901 T8899 pobj CD25,of
R6821 T8902 T8904 nummod FOXP3,minority
R6822 T8903 T8904 compound +,minority
R6823 T8904 T8896 advmod minority,caused
R6824 T8905 T8896 punct ",",caused
R6825 T8906 T8913 mark since,was
R6826 T8907 T8908 det the,number
R6827 T8908 T8913 nsubj number,was
R6828 T8909 T8908 prep of,number
R6829 T8910 T8912 nummod FOXP3,cells
R6830 T8911 T8912 nummod +,cells
R6831 T8912 T8909 pobj cells,of
R6832 T8913 T8896 advcl was,caused
R6833 T8914 T8913 acomp low/absent,was
R6834 T8915 T8914 prep in,low/absent
R6835 T8916 T8920 det the,CD45RA
R6836 T8917 T8918 amod purified,CD4
R6837 T8918 T8920 compound CD4,CD45RA
R6838 T8919 T8920 compound +,CD45RA
R6839 T8920 T8915 pobj CD45RA,in
R6840 T8921 T8923 amod +,cells
R6841 T8922 T8923 compound T,cells
R6842 T8923 T8915 pobj cells,in
R6843 T8924 T8940 punct (,confined
R6844 T8925 T8926 quantmod between,0
R6845 T8926 T8940 meta 0,confined
R6846 T8927 T8926 quantmod %,0
R6847 T8928 T8927 cc and,%
R6848 T8929 T8930 nummod 1,%
R6849 T8930 T8927 conj %,%
R6850 T8931 T8926 punct ),0
R6861 T8942 T8945 det the,cells
R6862 T8943 T8944 advmod highly,divided
R6863 T8944 T8945 amod divided,cells
R6864 T8945 T8941 pobj cells,to
R6865 T8946 T8889 punct .,mediated
R6866 T8947 T8949 nsubjpass CD25,down-regulated
R6867 T8948 T8949 auxpass was,down-regulated
R6868 T8949 T8949 ROOT down-regulated,down-regulated
R6869 T8950 T8949 prep in,down-regulated
R6870 T8951 T8950 pobj TGF-β,in
R6871 T8952 T8953 amod treated,cells
R6872 T8953 T8954 nsubj cells,compare
R6873 T8954 T8954 ROOT compare,compare
R6874 T8955 T8956 aux to,activated
R6875 T8956 T8958 amod activated,cells
R6876 T8957 T8958 compound T,cells
R6877 T8958 T8954 dobj cells,compare
R6878 T8959 T8958 punct ",",cells
R6879 T8960 T8961 nsubj which,was
R6880 T8961 T8958 relcl was,cells
R6881 T8962 T8963 advmod even,more
R6882 T8963 T8964 advmod more,pronounced
R6883 T8964 T8961 acomp pronounced,was
R6884 T8965 T8964 prep in,pronounced
R6885 T8966 T8965 pobj cells,in
R6886 T8967 T8966 acl cultured,cells
R6887 T8968 T8967 prep with,cultured
R6888 T8969 T8968 pobj TGF-β,with
R6889 T8970 T8969 cc and,TGF-β
R6890 T8971 T8969 conj IL-4,TGF-β
R6891 T8972 T8954 punct .,compare
R6892 T8973 T8974 det The,addition
R6893 T8974 T8980 nsubj addition,decreased
R6894 T8975 T8974 prep of,addition
R6895 T8976 T8975 pobj IL-4,of
R6896 T8977 T8976 prep to,IL-4
R6897 T8978 T8979 amod iTreg-driving,conditions
R6898 T8979 T8977 pobj conditions,to
R6899 T8980 T8980 ROOT decreased,decreased
R6900 T8981 T8982 det the,number
R6901 T8982 T8980 dobj number,decreased
R6902 T8983 T8982 prep of,number
R6903 T8984 T8986 nummod FOXP3,cells
R6904 T8985 T8986 nummod +,cells
R6905 T8986 T8983 pobj cells,of
R6906 T8987 T8989 punct (,6B
R6907 T8988 T8989 compound Figure,6B
R6908 T8989 T8986 appos 6B,cells
R6909 T8990 T8980 punct ),decreased
R6910 T8991 T8980 punct .,decreased
R6911 T8992 T9009 prep In,nonoverlapping
R6912 T8993 T8992 pobj line,In
R6913 T8994 T8993 prep with,line
R6914 T8995 T8997 det the,findings
R6915 T8996 T8997 amod previous,findings
R6916 T8997 T8994 pobj findings,with
R6917 T8998 T9009 punct ",",nonoverlapping
R6918 T8999 T9000 det the,IL-4
R6919 T9000 T9009 nsubj IL-4,nonoverlapping
R6920 T9001 T9000 acl producing,IL-4
R6921 T9002 T9001 dobj cells,producing
R6922 T9003 T9002 cc and,cells
R6923 T9004 T9007 det the,cells
R6924 T9005 T9007 nummod FOXP3,cells
R6925 T9006 T9007 amod expressing,cells
R6926 T9007 T9002 conj cells,cells
R6927 T9008 T9009 aux are,nonoverlapping
R6928 T9009 T9009 ROOT nonoverlapping,nonoverlapping
R6929 T9010 T9009 dobj populations,nonoverlapping
R6936 T9017 T9015 xcomp act,known
R6937 T9018 T9017 prep as,act
R6938 T9019 T9020 det a,repressor
R6939 T9020 T9018 pobj repressor,as
R6940 T9021 T9020 prep of,repressor
R6941 T9022 T9023 compound cytokine,expression
R6942 T9023 T9021 pobj expression,of
R6943 T9024 T9026 nmod [,]
R6944 T9025 T9026 nummod 20,]
R6945 T9026 T9020 appos ],repressor
R6946 T9027 T9030 punct ",",analyzed
R6947 T9028 T9030 nsubj we,analyzed
R6948 T9029 T9030 advmod therefore,analyzed
R6949 T9030 T9030 ROOT analyzed,analyzed
R6950 T9031 T9030 dobj GATA3,analyzed
R6951 T9032 T9031 cc and,GATA3
R6952 T9033 T9034 compound FOXP3,expression
R6953 T9034 T9031 conj expression,GATA3
R6954 T9035 T9030 punct .,analyzed
R6955 T9036 T9038 det The,kinetic
R6956 T9037 T9038 compound expression,kinetic
R6957 T9038 T9043 nsubj kinetic,exposed
R6958 T9039 T9038 prep of,kinetic
R6959 T9040 T9042 amod naive,cells
R6960 T9041 T9042 compound T,cells
R6961 T9042 T9039 pobj cells,of
R6962 T9043 T9048 csubj exposed,demonstrated
R6963 T9044 T9043 prep to,exposed
R6964 T9045 T9044 pobj IL-4,to
R6965 T9046 T9045 cc and,IL-4
R6966 T9047 T9045 conj TGF-β,IL-4
R6967 T9048 T9048 ROOT demonstrated,demonstrated
R6968 T9049 T9055 mark that,found
R6969 T9050 T9055 nsubjpass GATA3,found
R6970 T9051 T9050 cc and,GATA3
R6971 T9052 T9050 conj FOXP3,GATA3
R6972 T9053 T9055 auxpass are,found
R6973 T9054 T9055 advmod initially,found
R6974 T9055 T9048 ccomp found,demonstrated
R6975 T9056 T9055 prep in,found
R6976 T9057 T9058 amod separate,populations
R6977 T9058 T9056 pobj populations,in
R6978 T9059 T9060 punct (,day
R6979 T9060 T9055 npadvmod day,found
R6980 T9061 T9060 nummod 2,day
R6981 T9062 T9060 punct ),day
R6982 T9063 T9055 punct ",",found
R6983 T9064 T9055 cc but,found
R6984 T9065 T9066 advmod transiently,co-express
R6985 T9066 T9068 nummod co-express,factors
R6986 T9067 T9068 det both,factors
R6987 T9068 T9048 dobj factors,demonstrated
R6988 T9069 T9068 punct (,factors
R6989 T9070 T9068 appos days,factors
R6990 T9071 T9070 nummod 4,days
R6991 T9072 T9070 appos 8,days
R6992 T9073 T9070 punct ),days
R6993 T9074 T9068 punct ",",factors
R6994 T9075 T9087 prep before,day
R6995 T9076 T9075 pcomp establishing,before
R6996 T9077 T9078 amod separate,populations
R6997 T9078 T9076 dobj populations,establishing
R6998 T9079 T9076 prep at,establishing
R6999 T9080 T9081 det the,end
R7000 T9081 T9079 pobj end,at
R7001 T9082 T9081 prep of,end
R7002 T9083 T9085 det the,process
R7003 T9084 T9085 compound differentiation,process
R7004 T9085 T9082 pobj process,of
R7005 T9086 T9087 punct (,day
R7006 T9087 T9068 appos day,factors
R7007 T9088 T9087 nummod 10,day
R7008 T9089 T9068 punct ;,factors
R7009 T9090 T9091 compound Figure,6C
R7010 T9091 T9068 appos 6C,factors
R7011 T9092 T9068 punct ),factors
R7012 T9093 T9048 punct ",",demonstrated
R7013 T9094 T9048 advcl suggesting,demonstrated
R7014 T9095 T9097 mark that,inhibits
R7015 T9096 T9097 nsubj GATA3,inhibits
R7016 T9097 T9094 ccomp inhibits,suggesting
R7017 T9098 T9099 det the,development
R7018 T9099 T9097 dobj development,inhibits
R7019 T9100 T9099 prep of,development
R7020 T9101 T9102 compound iTreg,cells
R7021 T9102 T9100 pobj cells,of
R7022 T9103 T9097 prep by,inhibits
R7023 T9104 T9103 pcomp repressing,by
R7024 T9105 T9104 dobj FOXP3,repressing
R7025 T9106 T9048 punct .,demonstrated
R7026 T9107 T9108 det These,results
R7027 T9108 T9109 nsubj results,showed
R7028 T9109 T9109 ROOT showed,showed
R7029 T9110 T9129 mark that,acting
R7030 T9111 T9112 compound IL-4,acts
R7031 T9112 T9129 nsubj acts,acting
R7032 T9113 T9112 prep in,acts
R7033 T9114 T9113 pobj vitro,in
R7034 T9115 T9112 prep as,acts
R7035 T9116 T9117 det an,inhibitor
R7036 T9117 T9115 pobj inhibitor,as
R7037 T9118 T9117 prep of,inhibitor
R7038 T9119 T9120 nummod FOXP3,expression
R7039 T9120 T9118 pobj expression,of
R7040 T9121 T9112 punct ",",acts
R7041 T9122 T9112 prep without,acts
R7042 T9123 T9122 pcomp interfering,without
R7043 T9124 T9123 prep with,interfering
R7044 T9125 T9126 amod TGF-β,signaling
R7045 T9126 T9124 pobj signaling,with
R7046 T9127 T9126 punct ",",signaling
R7047 T9128 T9129 advmod probably,acting
R7048 T9129 T9109 ccomp acting,showed
R7049 T9130 T9129 prep at,acting
R7050 T9131 T9132 det the,level
R7051 T9132 T9130 pobj level,at
R7052 T9133 T9132 prep of,level
R7053 T9134 T9135 compound transcription,factors
R7054 T9135 T9133 pobj factors,of
R7055 T9136 T9129 punct ",",acting
R7056 T9137 T9129 cc and,acting
R7057 T9138 T9139 advmod possibly,by
R7058 T9139 T9129 conj by,acting
R7059 T9140 T9142 det a,mechanism
R7060 T9141 T9142 amod GATA3-dependent,mechanism
R7061 T9142 T9139 pobj mechanism,by
R7062 T9143 T9109 punct .,showed
R7826 T10354 T10356 det a,role
R7827 T10355 T10356 amod potential,role
R7828 T10356 T10353 dobj role,hypothesized
R7829 T10357 T10356 prep for,role
R7830 T10358 T10357 pobj GATA3,for
R7831 T10359 T10356 prep in,role
R7832 T10360 T10359 pcomp repressing,in
R7833 T10361 T10360 dobj FOXP3,repressing
R7834 T10362 T10353 punct .,hypothesized
R7835 T10363 T10366 advmod Besides,s
R7836 T10364 T10365 compound GATA3,′
R7837 T10365 T10366 nsubj ′,s
R7838 T10366 T10376 advcl s,s
R7839 T10367 T10369 amod well-known,effect
R7840 T10368 T10369 amod positive,effect
R7841 T10369 T10366 dobj effect,s
R7842 T10370 T10369 prep on,effect
R7843 T10371 T10372 compound gene,regulation
R7844 T10372 T10370 pobj regulation,on
R7845 T10373 T10372 punct ",",regulation
R7846 T10374 T10375 compound GATA3,′
R7847 T10375 T10376 nsubj ′,s
R7848 T10376 T10376 ROOT s,s
R7849 T10377 T10378 amod repressive,capabilities
R7850 T10378 T10381 nsubjpass capabilities,shown
R7851 T10379 T10381 auxpass were,shown
R7852 T10380 T10381 advmod previously,shown
R7853 T10381 T10376 ccomp shown,s
R7854 T10382 T10383 aux to,restrict
R7855 T10383 T10381 xcomp restrict,shown
R7856 T10384 T10385 amod Th1,commitment
R7857 T10385 T10383 dobj commitment,restrict
R7858 T10386 T10383 prep by,restrict
R7859 T10387 T10386 pcomp inhibiting,by
R7860 T10388 T10389 compound STAT4,expression
R7861 T10389 T10387 dobj expression,inhibiting
R7862 T10390 T10392 compound [,]
R7863 T10391 T10392 compound "2,21",]
R7874 T10402 T10376 punct .,s
R7875 T10403 T10404 aux To,investigate
R7876 T10404 T10414 advcl investigate,transduced
R7877 T10405 T10409 mark whether,inhibit
R7878 T10406 T10409 nsubj GATA3,inhibit
R7879 T10407 T10409 aux can,inhibit
R7880 T10408 T10409 advmod directly,inhibit
R7881 T10409 T10404 ccomp inhibit,investigate
R7882 T10410 T10411 nummod FOXP3,induction
R7883 T10411 T10409 dobj induction,inhibit
R7884 T10412 T10414 punct ",",transduced
R7885 T10413 T10414 nsubj we,transduced
R7886 T10414 T10414 ROOT transduced,transduced
R7887 T10415 T10414 dobj GATA3,transduced
R7888 T10416 T10415 cc or,GATA3
R7889 T10417 T10419 det a,GATA3
R7890 T10418 T10419 amod truncated,GATA3
R7891 T10419 T10415 conj GATA3,GATA3
R7892 T10420 T10419 acl lacking,GATA3
R7893 T10421 T10423 det the,domain
R7894 T10422 T10423 amod DNA-binding,domain
R7895 T10423 T10420 dobj domain,lacking
R7896 T10424 T10423 prep in,domain
R7897 T10425 T10429 amod human,CD45RA
R7898 T10426 T10429 amod primary,CD45RA
R7899 T10427 T10429 compound CD4,CD45RA
R7900 T10428 T10429 compound +,CD45RA
R7901 T10429 T10424 pobj CD45RA,in
R7902 T10430 T10414 conj +,transduced
R7903 T10431 T10432 compound T,cells
R7904 T10432 T10430 dobj cells,+
R7905 T10433 T10430 advcl using,+
R7906 T10434 T10439 det a,GATA3
R7907 T10435 T10438 amod TAT-fused,expressed
R7908 T10436 T10438 punct ",",expressed
R7909 T10437 T10438 advmod recombinantly,expressed
R7910 T10438 T10439 amod expressed,GATA3
R7911 T10439 T10433 dobj GATA3,using
R7912 T10440 T10430 punct .,+
R7913 T10441 T10447 prep After,activated
R7914 T10442 T10441 pobj transduction,After
R7915 T10443 T10447 punct ",",activated
R7916 T10444 T10445 det the,cells
R7917 T10445 T10447 nsubjpass cells,activated
R7918 T10446 T10447 auxpass were,activated
R7919 T10447 T10447 ROOT activated,activated
R7920 T10448 T10447 prep with,activated
R7921 T10449 T10451 amod soluble,/
R7922 T10450 T10451 amod anti-CD3,/
R7923 T10451 T10448 pobj /,with
R7924 T10452 T10451 nummod CD28,/
R7925 T10453 T10451 prep in,/
R7926 T10454 T10455 det the,presence
R7927 T10455 T10453 pobj presence,in
R7928 T10456 T10455 cc or,presence
R7929 T10457 T10455 conj absence,presence
R7930 T10458 T10457 prep of,absence
R7931 T10459 T10458 pobj TGF-β,of
R7932 T10460 T10447 punct .,activated
R7933 T10461 T10464 nsubjpass TAT-GATA3,transduced
R7934 T10462 T10464 auxpass was,transduced
R7935 T10463 T10464 advmod successfully,transduced
R7936 T10464 T10464 ROOT transduced,transduced
R7937 T10465 T10464 prep in,transduced
R7938 T10466 T10470 det a,manner
R7939 T10467 T10470 amod homogeneous,manner
R7940 T10468 T10467 cc and,homogeneous
R7941 T10469 T10467 conj dose-dependent,homogeneous
R7942 T10470 T10465 pobj manner,in
R7943 T10471 T10470 prep into,manner
R7944 T10472 T10476 amod human,cells
R7945 T10473 T10476 nmod CD4,cells
R7946 T10474 T10476 nummod +,cells
R7947 T10475 T10476 compound T,cells
R7948 T10476 T10471 pobj cells,into
R7949 T10477 T10479 punct (,7A
R7950 T10478 T10479 compound Figure,7A
R7951 T10479 T10464 npadvmod 7A,transduced
R7952 T10480 T10482 punct ",",panel
R7953 T10481 T10482 amod upper,panel
R7954 T10482 T10479 appos panel,7A
R7955 T10483 T10479 punct ),7A
R7956 T10484 T10464 punct .,transduced
R7957 T10485 T10488 nummod TAT-GATA3,expression
R7958 T10486 T10488 amod reduced,expression
R7959 T10487 T10488 nummod FOXP3,expression
R7960 T10488 T10488 ROOT expression,expression
R7961 T10489 T10488 prep in,expression
R7962 T10490 T10492 det a,manner
R7963 T10491 T10492 amod dose-dependent,manner
R7964 T10492 T10489 pobj manner,in
R7965 T10493 T10488 punct ",",expression
R7966 T10494 T10505 mark whereas,affect
R7967 T10495 T10497 det a,domain
R7968 T10496 T10497 amod DNA-binding,domain
R7969 T10497 T10505 nsubj domain,affect
R7970 T10498 T10499 amod truncated,version
R7971 T10499 T10497 appos version,domain
R7972 T10500 T10499 punct (,version
R7973 T10501 T10499 appos TAT-ΔDBD-GATA3,version
R7974 T10502 T10499 punct ),version
R7975 T10503 T10505 aux did,affect
R7976 T10504 T10505 neg not,affect
R7977 T10505 T10488 advcl affect,expression
R7978 T10506 T10507 nummod FOXP3,expression
R7979 T10507 T10505 dobj expression,affect
R7980 T10508 T10505 prep as,affect
R7981 T10509 T10508 prep compared,as
R7982 T10510 T10509 prep with,compared
R7983 T10511 T10510 pobj expression,with
R7984 T10512 T10511 prep in,expression
R7985 T10513 T10514 amod untransduced,cells
R7986 T10514 T10512 pobj cells,in
R7987 T10515 T10517 punct (,7A
R7988 T10516 T10517 compound Figure,7A
R7989 T10517 T10511 appos 7A,expression
R7990 T10518 T10505 punct ),affect
R7991 T10519 T10488 punct .,expression
R7992 T10520 T10524 prep In,analyzed
R7993 T10521 T10520 pobj addition,In
R7994 T10522 T10524 punct ",",analyzed
R7995 T10523 T10524 nsubj we,analyzed
R7996 T10524 T10524 ROOT analyzed,analyzed
R7997 T10525 T10527 det the,effect
R7998 T10526 T10527 amod inhibitory,effect
R7999 T10527 T10524 dobj effect,analyzed
R8000 T10528 T10527 prep of,effect
R8001 T10529 T10528 pobj GATA3,of
R8002 T10530 T10527 prep on,effect
R8003 T10531 T10530 pobj FOXP3,on
R8004 T10532 T10531 prep in,FOXP3
R8005 T10533 T10536 amod transgenic,mice
R8006 T10534 T10536 nmod DO11,mice
R8007 T10535 T10534 nummod .10,DO11
R8008 T10536 T10532 pobj mice,in
R8009 T10537 T10524 punct ",",analyzed
R8010 T10538 T10539 advmod constitutively,overexpressing
R8011 T10539 T10524 advcl overexpressing,analyzed
R8012 T10540 T10539 dobj GATA3,overexpressing
R8013 T10541 T10539 prep under,overexpressing
R8014 T10542 T10543 det the,control
R8015 T10543 T10541 pobj control,under
R8016 T10544 T10543 prep of,control
R8017 T10545 T10549 det the,region
R8018 T10546 T10549 compound CD2,region
R8019 T10547 T10548 compound locus,control
R8020 T10548 T10549 compound control,region
R8021 T10549 T10544 pobj region,of
R8022 T10550 T10551 punct (,DO11.10xCD2-GATA3
R8023 T10551 T10549 appos DO11.10xCD2-GATA3,region
R8024 T10552 T10551 punct ),DO11.10xCD2-GATA3
R8025 T10553 T10524 punct .,analyzed
R8026 T10554 T10556 det The,selection
R8027 T10555 T10556 amod thymic,selection
R8028 T10556 T10561 nsubj selection,is
R8029 T10557 T10556 prep into,selection
R8030 T10558 T10560 det the,lineage
R8031 T10559 T10560 compound CD4,lineage
R8032 T10560 T10557 pobj lineage,into
R8033 T10561 T10561 ROOT is,is
R8034 T10562 T10563 advmod largely,intact
R8035 T10563 T10561 acomp intact,is
R8036 T10564 T10563 prep in,intact
R8037 T10565 T10564 pobj DO11.10xCD2-GATA3,in
R8038 T10566 T10568 punct (,Hendriks
R8039 T10567 T10568 compound RW,Hendriks
R8040 T10568 T10565 appos Hendriks,DO11.10xCD2-GATA3
R8041 T10569 T10571 punct ",",data
R8042 T10570 T10571 amod unpublished,data
R8043 T10571 T10568 appos data,Hendriks
R8044 T10572 T10568 punct ),Hendriks
R8045 T10573 T10561 punct .,is
R8046 T10574 T10575 det These,mice
R8047 T10575 T10576 nsubj mice,develop
R8048 T10576 T10576 ROOT develop,develop
R8049 T10577 T10576 dobj lymphomas,develop
R8050 T10578 T10576 prep at,develop
R8051 T10579 T10581 det an,age
R8052 T10580 T10581 amod older,age
R8053 T10581 T10578 pobj age,at
R8054 T10582 T10576 punct ",",develop
R8055 T10583 T10576 cc but,develop
R8056 T10584 T10590 nsubjpass signs,described
R8057 T10585 T10584 prep of,signs
R8058 T10586 T10587 amod autoimmune,disease
R8059 T10587 T10585 pobj disease,of
R8060 T10588 T10590 auxpass were,described
R8061 T10589 T10590 neg not,described
R8062 T10590 T10576 conj described,develop
R8063 T10591 T10593 nmod [,]
R8064 T10592 T10593 nummod 22,]
R8065 T10593 T10590 dobj ],described
R8066 T10594 T10576 punct .,develop
R8067 T10595 T10596 aux To,investigate
R8068 T10596 T10610 advcl investigate,were
R8069 T10597 T10598 det the,effect
R8070 T10598 T10596 dobj effect,investigate
R8071 T10599 T10598 prep of,effect
R8072 T10600 T10599 pobj GATA3,of
R8073 T10601 T10598 prep on,effect
R8074 T10602 T10601 pobj iTreg,on
R8075 T10603 T10602 punct ",",iTreg
R8076 T10604 T10608 compound CD4,CD25
R8077 T10605 T10608 compound +,CD25
R8078 T10606 T10608 compound CD62L,CD25
R8079 T10607 T10608 compound +,CD25
R8080 T10608 T10602 conj CD25,iTreg
R8081 T10609 T10610 nsubj cells,were
R8082 T10610 T10610 ROOT were,were
R8083 T10611 T10610 acomp isolated,were
R8084 T10612 T10611 punct ",",isolated
R8085 T10613 T10611 conj activated,isolated
R8086 T10614 T10613 prep with,activated
R8087 T10615 T10614 pobj OVA,with
R8088 T10616 T10613 prep in,activated
R8089 T10617 T10618 det the,presence
R8090 T10618 T10616 pobj presence,in
R8091 T10619 T10618 cc or,presence
R8092 T10620 T10618 conj absence,presence
R8093 T10621 T10620 prep of,absence
R8094 T10622 T10621 pobj TGF-β,of
R8095 T10623 T10620 punct ",",absence
R8096 T10624 T10620 cc and,absence
R8097 T10625 T10626 amod Foxp3,expression
R8098 T10626 T10620 conj expression,absence
R8099 T10627 T10628 auxpass was,analyzed
R8100 T10628 T10610 conj analyzed,were
R8101 T10629 T10628 prep after,analyzed
R8102 T10630 T10631 nummod 4,d
R8103 T10631 T10629 pobj d,after
R8104 T10632 T10610 punct .,were
R8105 T10633 T10637 det The,CD25
R8106 T10634 T10637 amod naive,CD25
R8107 T10635 T10637 compound CD4,CD25
R8108 T10636 T10637 compound +,CD25
R8109 T10637 T10637 ROOT CD25,CD25
R8110 T10638 T10639 nsubj cells,were
R8111 T10639 T10639 ROOT were,were
R8112 T10640 T10639 acomp Foxp3,were
R8113 T10641 T10643 punct (,data
R8114 T10642 T10643 amod unpublished,data
R8115 T10643 T10640 appos data,Foxp3
R8116 T10644 T10640 punct ),Foxp3
R8117 T10645 T10639 punct .,were
R8118 T10646 T10647 mark As,described
R8119 T10647 T10656 advcl described,Foxp3
R8120 T10648 T10647 prep for,described
R8121 T10649 T10651 det the,cells
R8122 T10650 T10651 amod human,cells
R8123 T10651 T10648 pobj cells,for
R8124 T10652 T10656 punct ",",Foxp3
R8125 T10653 T10656 nmod TGF-β,Foxp3
R8126 T10654 T10655 advmod dramatically,up-regulated
R8127 T10655 T10656 amod up-regulated,Foxp3
R8128 T10656 T10656 ROOT Foxp3,Foxp3
R8129 T10657 T10656 prep in,Foxp3
R8130 T10658 T10663 det the,mice
R8131 T10659 T10663 compound DO11,mice
R8132 T10660 T10661 nummod .10,littermate
R8133 T10661 T10662 compound littermate,control
R8134 T10662 T10663 compound control,mice
R8135 T10663 T10657 pobj mice,in
R8136 T10664 T10656 punct .,Foxp3
R8137 T10665 T10674 prep In,showed
R8138 T10666 T10665 pobj contrast,In
R8139 T10667 T10674 punct ",",showed
R8140 T10668 T10674 nsubj cells,showed
R8141 T10669 T10668 prep from,cells
R8142 T10670 T10673 det the,mice
R8143 T10671 T10673 nmod CD2-GATA3xDO11,mice
R8144 T10672 T10671 nummod .10,CD2-GATA3xDO11
R8145 T10673 T10669 pobj mice,from
R8146 T10674 T10674 ROOT showed,showed
R8147 T10675 T10676 advmod dramatically,reduced
R8148 T10676 T10678 amod reduced,expression
R8149 T10677 T10678 amod Foxp3,expression
R8150 T10678 T10674 dobj expression,showed
R8151 T10679 T10680 advmod when,activated
R8152 T10680 T10674 advcl activated,showed
R8153 T10681 T10680 prep with,activated
R8154 T10682 T10681 pobj TGF-β,with
R8155 T10683 T10682 cc and,TGF-β
R8156 T10684 T10682 conj OVA,TGF-β
R8157 T10685 T10687 punct (,7B
R8158 T10686 T10687 compound Figure,7B
R8159 T10687 T10684 appos 7B,OVA
R8160 T10688 T10687 punct ),7B
R8161 T10689 T10674 punct .,showed
R8162 T10690 T10691 det All,mice
R8163 T10691 T10692 nsubj mice,produced
R8164 T10692 T10704 ccomp produced,expressed
R8165 T10693 T10694 amod similar,amounts
R8166 T10694 T10692 dobj amounts,produced
R8167 T10695 T10694 prep of,amounts
R8168 T10696 T10695 pobj TGF-β,of
R8169 T10697 T10704 punct ;,expressed
R8170 T10698 T10704 prep in,expressed
R8171 T10699 T10698 pobj addition,in
R8172 T10700 T10704 punct ",",expressed
R8173 T10701 T10704 nsubjpass Smad7,expressed
R8174 T10702 T10704 auxpass was,expressed
R8175 T10703 T10704 advmod equally,expressed
R8176 T10704 T10704 ROOT expressed,expressed
R8177 T10705 T10707 nmod [,]
R8178 T10706 T10707 nummod 23,]
R8179 T10707 T10704 dobj ],expressed
R8180 T10708 T10707 prep in,]
R8181 T10709 T10710 compound T,cells
R8182 T10710 T10708 pobj cells,in
R8183 T10711 T10710 prep of,cells
R8184 T10712 T10713 preconj both,mice
R8185 T10713 T10711 pobj mice,of
R8186 T10714 T10713 conj strains,mice
R8187 T10715 T10717 punct (,7C
R8188 T10716 T10717 compound Figure,7C
R8189 T10717 T10714 appos 7C,strains
R8190 T10718 T10717 punct ),7C
R8191 T10719 T10704 punct ",",expressed
R8192 T10720 T10704 advcl indicating,expressed
R8193 T10721 T10723 amod intact,signaling
R8194 T10722 T10723 amod TGF-β,signaling
R8195 T10723 T10720 dobj signaling,indicating
R8196 T10724 T10704 punct .,expressed
R8197 T10725 T10730 advcl Taken,demonstrated
R8198 T10726 T10725 advmod together,Taken
R8199 T10727 T10730 punct ",",demonstrated
R8200 T10728 T10729 det these,results
R8201 T10729 T10730 nsubj results,demonstrated
R8202 T10730 T10730 ROOT demonstrated,demonstrated
R8203 T10731 T10733 det a,role
R8204 T10732 T10733 amod repressive,role
R8205 T10733 T10730 dobj role,demonstrated
R8206 T10734 T10733 prep of,role
R8207 T10735 T10738 amod IL-4-induced,factor
R8208 T10736 T10738 compound GATA3,factor
R8209 T10737 T10738 compound transcription,factor
R8210 T10738 T10734 pobj factor,of
R8211 T10739 T10730 prep in,demonstrated
R8212 T10740 T10741 det the,generation
R8213 T10741 T10739 pobj generation,in
R8214 T10742 T10741 prep of,generation
R8215 T10743 T10744 compound iTreg,cells
R8216 T10744 T10742 pobj cells,of
R8217 T10745 T10730 punct .,demonstrated
R9042 T12080 T12081 nummod GATA3,Represses
R9043 T12081 T12084 compound Represses,Promoter
R9044 T12082 T12083 det the,FOXP3
R9045 T12083 T12084 compound FOXP3,Promoter
R9046 T12084 T12086 nsubj Promoter,investigate
R9047 T12085 T12086 aux To,investigate
R9048 T12086 T12086 ROOT investigate,investigate
R9049 T12087 T12089 det the,mechanism
R9050 T12088 T12089 amod molecular,mechanism
R9051 T12089 T12086 dobj mechanism,investigate
R9052 T12090 T12089 prep of,mechanism
R9053 T12091 T12092 amod GATA3-mediated,repression
R9054 T12092 T12090 pobj repression,of
R9055 T12093 T12092 prep of,repression
R9056 T12094 T12095 compound human,FOXP3
R9057 T12095 T12093 pobj FOXP3,of
R9058 T12096 T12102 punct ",",studied
R9059 T12097 T12100 det the,promoter
R9060 T12098 T12100 amod human,promoter
R9061 T12099 T12100 nummod FOXP3,promoter
R9062 T12100 T12102 nsubjpass promoter,studied
R9063 T12101 T12102 auxpass was,studied
R9064 T12102 T12086 conj studied,investigate
R9065 T12103 T12102 cc and,studied
R9066 T12104 T12107 det a,site
R9067 T12105 T12107 amod palindromic,site
R9068 T12106 T12107 amod binding,site
R9069 T12107 T12111 nsubjpass site,discovered
R9070 T12108 T12107 prep for,site
R9071 T12109 T12108 pobj GATA3,for
R9072 T12110 T12111 auxpass was,discovered
R9073 T12111 T12102 conj discovered,studied
R9074 T12112 T12102 punct .,studied
R9075 T12113 T12115 det The,site
R9076 T12114 T12115 amod GATA-binding,site
R9077 T12115 T12117 nsubjpass site,located
R9078 T12116 T12117 auxpass is,located
R9079 T12117 T12117 ROOT located,located
R9080 T12118 T12120 nummod 303,upstream
R9081 T12119 T12120 compound bp,upstream
R9082 T12120 T12117 advmod upstream,located
R9083 T12121 T12120 prep from,upstream
R9084 T12122 T12123 det the,transcription
R9085 T12123 T12121 pobj transcription,from
R9086 T12124 T12125 compound start,site
R9087 T12125 T12131 nmod site,]
R9088 T12126 T12127 punct (,TSS
R9089 T12127 T12125 appos TSS,site
R9090 T12128 T12125 punct ),site
R9091 T12129 T12131 nmod [,]
R9092 T12130 T12131 nummod 24,]
R9093 T12131 T12117 npadvmod ],located
R9094 T12132 T12117 punct .,located
R9095 T12133 T12134 det This,site
R9096 T12134 T12137 nsubjpass site,conserved
R9097 T12135 T12137 auxpass is,conserved
R9098 T12136 T12137 advmod highly,conserved
R9099 T12137 T12137 ROOT conserved,conserved
R9100 T12138 T12137 prep between,conserved
R9101 T12139 T12138 pobj humans,between
R9102 T12140 T12139 punct ",",humans
R9103 T12141 T12139 conj mice,humans
R9104 T12142 T12137 punct ",",conserved
R9105 T12143 T12137 cc and,conserved
R9106 T12144 T12137 conj rats,conserved
R9107 T12145 T12146 punct (,Figure
R9108 T12146 T12144 appos Figure,rats
R9109 T12147 T12146 nummod S4,Figure
R9110 T12148 T12144 punct ),rats
R9111 T12149 T12137 cc and,conserved
R9112 T12150 T12152 aux may,play
R9113 T12151 T12152 advmod therefore,play
R9114 T12152 T12137 conj play,conserved
R9115 T12153 T12155 det an,role
R9116 T12154 T12155 amod important,role
R9117 T12155 T12152 dobj role,play
R9118 T12156 T12152 prep in,play
R9119 T12157 T12158 amod FOXP3,regulation
R9120 T12158 T12156 pobj regulation,in
R9121 T12159 T12137 punct .,conserved
R9122 T12160 T12162 det The,relevance
R9123 T12161 T12162 amod functional,relevance
R9124 T12162 T12167 nsubjpass relevance,studied
R9125 T12163 T12162 prep of,relevance
R9126 T12164 T12165 det this,site
R9127 T12165 T12163 pobj site,of
R9128 T12166 T12167 auxpass was,studied
R9129 T12167 T12167 ROOT studied,studied
R9130 T12168 T12167 advcl using,studied
R9131 T12169 T12170 det a,FOXP3-promoter
R9132 T12170 T12171 nsubj FOXP3-promoter,construct
R9133 T12171 T12168 xcomp construct,using
R9134 T12172 T12174 nmod [,]
R9135 T12173 T12174 nummod 24,]
R9136 T12174 T12171 dobj ],construct
R9137 T12175 T12167 punct .,studied
R9138 T12176 T12177 nsubj We,transfected
R9139 T12177 T12177 ROOT transfected,transfected
R9140 T12178 T12183 amod human,cells
R9141 T12179 T12183 amod primary,cells
R9142 T12180 T12183 compound CD4,cells
R9143 T12181 T12183 nummod +,cells
R9144 T12182 T12183 compound T,cells
R9145 T12183 T12177 dobj cells,transfected
R9146 T12184 T12177 punct ",",transfected
R9147 T12185 T12177 prep in,transfected
R9148 T12186 T12189 amod vitro,cells
R9149 T12187 T12189 amod differentiated,cells
R9150 T12188 T12189 amod Th2,cells
R9151 T12189 T12185 pobj cells,in
R9152 T12190 T12177 punct ",",transfected
R9153 T12191 T12177 cc and,transfected
R9154 T12192 T12193 compound Jurkat,cells
R9155 T12193 T12177 conj cells,transfected
R9156 T12194 T12198 punct (,known
R9157 T12195 T12196 compound Jurkat,cells
R9158 T12196 T12198 nsubjpass cells,known
R9159 T12197 T12198 auxpass are,known
R9160 T12198 T12193 relcl known,cells
R9161 T12199 T12201 aux to,express
R9162 T12200 T12201 advmod constitutively,express
R9163 T12201 T12198 xcomp express,known
R9164 T12202 T12203 compound GATA3,[
R9165 T12203 T12201 dobj [,express
R9166 T12204 T12205 nummod "25,26",]
R9167 T12205 T12203 appos ],[
R9168 T12206 T12203 punct ),[
R9169 T12207 T12198 punct ",",known
R9170 T12208 T12177 cc and,transfected
R9171 T12209 T12210 nsubj we,measured
R9172 T12210 T12177 conj measured,transfected
R9173 T12211 T12213 nummod FOXP3,activity
R9174 T12212 T12213 compound promoter,activity
R9175 T12213 T12210 dobj activity,measured
R9176 T12214 T12210 punct .,measured
R9177 T12215 T12216 det The,promoter
R9178 T12216 T12217 nsubj promoter,was
R9179 T12217 T12217 ROOT was,was
R9180 T12218 T12217 neg not,was
R9181 T12219 T12217 acomp active,was
R9182 T12220 T12219 prep in,active
R9183 T12221 T12224 det the,line
R9184 T12222 T12223 compound GATA3-expressing,cell
R9185 T12223 T12224 compound cell,line
R9186 T12224 T12220 pobj line,in
R9187 T12225 T12224 appos Jurkat,line
R9188 T12226 T12220 cc or,in
R9189 T12227 T12220 conj in,in
R9190 T12228 T12229 det the,in
R9191 T12229 T12227 pobj in,in
R9192 T12230 T12232 amod vitro-differentiated,cells
R9193 T12231 T12232 amod Th2,cells
R9194 T12232 T12229 pobj cells,in
R9195 T12233 T12217 punct ",",was
R9196 T12234 T12237 mark whereas,was
R9197 T12235 T12236 det the,construct
R9198 T12236 T12237 nsubj construct,was
R9199 T12237 T12217 advcl was,was
R9200 T12238 T12237 acomp active,was
R9201 T12239 T12238 prep in,active
R9202 T12240 T12242 det the,cells
R9203 T12241 T12242 compound CD4,cells
R9204 T12242 T12239 pobj cells,in
R9205 T12243 T12237 punct ",",was
R9206 T12244 T12245 nsubj which,express
R9207 T12245 T12217 conj express,was
R9208 T12246 T12248 det a,amount
R9209 T12247 T12248 amod lower,amount
R9210 T12248 T12245 dobj amount,express
R9211 T12249 T12248 prep of,amount
R9212 T12250 T12249 pobj GATA3,of
R9213 T12251 T12253 punct (,8A
R9214 T12252 T12253 compound Figure,8A
R9215 T12253 T12250 appos 8A,GATA3
R9216 T12254 T12253 punct ),8A
R9217 T12255 T12217 punct .,was
R9218 T12256 T12263 nsubj Overexpression,diminished
R9219 T12257 T12256 prep of,Overexpression
R9220 T12258 T12257 pobj GATA3,of
R9221 T12259 T12256 prep in,Overexpression
R9222 T12260 T12262 amod naive,cells
R9223 T12261 T12262 compound T,cells
R9224 T12262 T12259 pobj cells,in
R9225 T12263 T12263 ROOT diminished,diminished
R9226 T12264 T12265 compound luciferase,activity
R9227 T12265 T12263 dobj activity,diminished
R9228 T12266 T12265 prep of,activity
R9229 T12267 T12269 det the,promoter
R9230 T12268 T12269 nummod FOXP3,promoter
R9231 T12269 T12266 pobj promoter,of
R9232 T12270 T12263 prep compared,diminished
R9233 T12271 T12270 prep with,compared
R9234 T12272 T12274 det the,vector
R9235 T12273 T12274 compound control,vector
R9236 T12274 T12271 pobj vector,with
R9237 T12275 T12277 punct (,8B
R9238 T12276 T12277 compound Figure,8B
R9239 T12277 T12274 appos 8B,vector
R9240 T12278 T12277 punct ),8B
R9241 T12279 T12263 punct .,diminished
R9242 T12280 T12282 aux To,address
R9243 T12281 T12282 advmod further,address
R9244 T12282 T12291 advcl address,inserted
R9245 T12283 T12284 det the,function
R9246 T12284 T12282 dobj function,address
R9247 T12285 T12284 prep of,function
R9248 T12286 T12288 det the,site
R9249 T12287 T12288 compound GATA3,site
R9250 T12288 T12285 pobj site,of
R9251 T12289 T12291 punct ",",inserted
R9252 T12290 T12291 nsubj we,inserted
R9253 T12291 T12291 ROOT inserted,inserted
R9254 T12292 T12294 det a,mutation
R9255 T12293 T12294 amod site-specific,mutation
R9256 T12294 T12291 dobj mutation,inserted
R9257 T12295 T12294 acl deleting,mutation
R9258 T12296 T12298 det the,site
R9259 T12297 T12298 amod GATA3-binding,site
R9260 T12298 T12295 dobj site,deleting
R9261 T12299 T12291 punct .,inserted
R9262 T12300 T12301 det This,mutation
R9263 T12301 T12302 nsubj mutation,increased
R9264 T12302 T12302 ROOT increased,increased
R9265 T12303 T12304 compound luciferase,activity
R9266 T12304 T12302 dobj activity,increased
R9267 T12305 T12304 prep by,activity
R9268 T12306 T12305 pobj 3-fold,by
R9269 T12307 T12306 prep in,3-fold
R9270 T12308 T12307 pobj memory,in
R9271 T12309 T12311 compound CD4,CD45RO
R9272 T12310 T12311 compound +,CD45RO
R9273 T12311 T12312 nsubj CD45RO,+
R9274 T12312 T12302 conj +,increased
R9275 T12313 T12314 compound T,cells
R9276 T12314 T12312 dobj cells,+
R9277 T12315 T12312 punct ",",+
R9278 T12316 T12320 mark whereas,observed
R9279 T12317 T12318 det no,difference
R9280 T12318 T12320 nsubjpass difference,observed
R9281 T12319 T12320 auxpass was,observed
R9282 T12320 T12312 advcl observed,+
R9283 T12321 T12320 prep in,observed
R9284 T12322 T12331 amod naive,cells
R9285 T12323 T12331 punct (,cells
R9286 T12324 T12331 nummod GATA3,cells
R9287 T12325 T12324 punct ),GATA3
R9288 T12326 T12331 nummod CD4,cells
R9289 T12327 T12331 nummod +,cells
R9290 T12328 T12331 nmod CD45RA,cells
R9291 T12329 T12331 nummod +,cells
R9292 T12330 T12331 compound T,cells
R9293 T12331 T12321 pobj cells,in
R9294 T12332 T12320 punct ",",observed
R9295 T12333 T12320 advcl revealing,observed
R9296 T12334 T12336 det a,activity
R9297 T12335 T12336 compound repressor,activity
R9298 T12336 T12333 dobj activity,revealing
R9299 T12337 T12336 prep of,activity
R9300 T12338 T12337 pobj GATA3,of
R9301 T12339 T12333 prep on,revealing
R9302 T12340 T12342 det the,promoter
R9303 T12341 T12342 nummod FOXP3,promoter
R9304 T12342 T12339 pobj promoter,on
R9305 T12343 T12345 punct (,8C
R9306 T12344 T12345 compound Figure,8C
R9307 T12345 T12342 appos 8C,promoter
R9308 T12346 T12345 punct ),8C
R9309 T12347 T12302 punct .,increased
R9310 T12348 T12350 advmod Furthermore,binds
R9311 T12349 T12350 amod GATA3,binds
R9312 T12350 T12350 ROOT binds,binds
R9313 T12351 T12352 advmod directly,to
R9314 T12352 T12350 prep to,binds
R9315 T12353 T12355 det the,promoter
R9316 T12354 T12355 nummod FOXP3,promoter
R9317 T12355 T12352 pobj promoter,to
R9318 T12356 T12357 mark as,investigated
R9319 T12357 T12350 advcl investigated,binds
R9320 T12358 T12357 agent by,investigated
R9321 T12359 T12360 amod pull-down,assay
R9322 T12360 T12358 pobj assay,by
R9323 T12361 T12350 punct .,binds
R9324 T12362 T12363 compound HEK,cells
R9325 T12363 T12366 nsubjpass cells,transfected
R9326 T12364 T12366 auxpass were,transfected
R9327 T12365 T12366 advmod transiently,transfected
R9328 T12366 T12366 ROOT transfected,transfected
R9329 T12367 T12380 mark with,incubated
R9330 T12368 T12380 nsubjpass GATA3,incubated
R9331 T12369 T12368 cc or,GATA3
R9332 T12370 T12372 det a,vector
R9333 T12371 T12372 compound control,vector
R9334 T12372 T12368 conj vector,GATA3
R9335 T12373 T12372 punct ",",vector
R9336 T12374 T12372 cc and,vector
R9337 T12375 T12372 conj increasing,vector
R9338 T12376 T12375 dobj amounts,increasing
R9339 T12377 T12376 prep of,amounts
R9340 T12378 T12377 pobj lysates,of
R9341 T12379 T12380 auxpass were,incubated
R9342 T12380 T12366 advcl incubated,transfected
R9343 T12381 T12380 prep with,incubated
R9344 T12382 T12381 pobj oligonucleotides,with
R9345 T12383 T12382 acl containing,oligonucleotides
R9346 T12384 T12386 det the,site
R9347 T12385 T12386 compound GATA3,site
R9348 T12386 T12383 dobj site,containing
R9349 T12387 T12386 prep of,site
R9350 T12388 T12390 det the,promoter
R9351 T12389 T12390 nummod FOXP3,promoter
R9352 T12390 T12387 pobj promoter,of
R9353 T12391 T12390 cc or,promoter
R9354 T12392 T12394 det a,oligonucleotide
R9355 T12393 T12394 compound control,oligonucleotide
R9356 T12394 T12390 conj oligonucleotide,promoter
R9357 T12395 T12394 prep with,oligonucleotide
R9358 T12396 T12399 det a,site
R9359 T12397 T12399 amod mutated,site
R9360 T12398 T12399 amod GATA3-binding,site
R9361 T12399 T12395 pobj site,with
R9362 T12400 T12366 punct .,transfected
R9363 T12401 T12408 mark After,detected
R9364 T12402 T12408 det the,detected
R9365 T12403 T12408 amod pull-down,detected
R9366 T12404 T12408 punct ",",detected
R9367 T12405 T12406 amod GATA3,binding
R9368 T12406 T12408 nsubjpass binding,detected
R9369 T12407 T12408 auxpass was,detected
R9370 T12408 T12408 ROOT detected,detected
R9371 T12409 T12408 agent by,detected
R9372 T12410 T12411 amod Western,blot
R9373 T12411 T12409 pobj blot,by
R9374 T12412 T12408 punct .,detected
R9375 T12413 T12422 advmod Similarly,subjected
R9376 T12414 T12422 punct ",",subjected
R9377 T12415 T12417 amod GATA3-expressing,cells
R9378 T12416 T12417 amod Th2,cells
R9379 T12417 T12422 nsubjpass cells,subjected
R9380 T12418 T12417 cc and,cells
R9381 T12419 T12420 compound iTreg,cells
R9382 T12420 T12417 conj cells,cells
R9383 T12421 T12422 auxpass were,subjected
R9384 T12422 T12422 ROOT subjected,subjected
R9385 T12423 T12422 prep to,subjected
R9386 T12424 T12425 det this,approach
R9387 T12425 T12423 pobj approach,to
R9388 T12426 T12422 punct .,subjected
R9389 T12427 T12428 advmod Only,HEK
R9390 T12428 T12429 compound HEK,cells
R9391 T12429 T12435 nsubj cells,showed
R9392 T12430 T12429 acl overexpressing,cells
R9393 T12431 T12434 nmod GATA3,cells
R9394 T12432 T12431 cc and,GATA3
R9395 T12433 T12431 conj Th2,GATA3
R9396 T12434 T12430 dobj cells,overexpressing
R9397 T12435 T12435 ROOT showed,showed
R9398 T12436 T12437 amod GATA3-binding,activity
R9399 T12437 T12435 dobj activity,showed
R9400 T12438 T12439 punct (,Figure
R9401 T12439 T12437 appos Figure,activity
R9402 T12440 T12439 nummod 8D,Figure
R9403 T12441 T12439 cc and,Figure
R9404 T12442 T12439 conj 8E,Figure
R9405 T12443 T12437 punct ),activity
R9406 T12444 T12435 punct .,showed
R9407 T12445 T12446 det These,experiments
R9408 T12446 T12447 nsubj experiments,demonstrated
R9409 T12447 T12447 ROOT demonstrated,demonstrated
R9410 T12448 T12450 mark that,binds
R9411 T12449 T12450 nsubj GATA3,binds
R9412 T12450 T12447 ccomp binds,demonstrated
R9413 T12451 T12454 det the,promoter
R9414 T12452 T12454 amod palindromic,promoter
R9415 T12453 T12454 nummod FOXP3,promoter
R9416 T12454 T12450 dobj promoter,binds
R9417 T12455 T12447 punct .,demonstrated
R9418 T12456 T12457 aux To,gain
R9419 T12457 T12466 advcl gain,performed
R9420 T12458 T12457 dobj insights,gain
R9421 T12459 T12458 prep into,insights
R9422 T12460 T12466 dep the,performed
R9423 T12461 T12466 prep in,performed
R9424 T12462 T12463 compound vivo,situation
R9425 T12463 T12461 pobj situation,in
R9426 T12464 T12466 punct ",",performed
R9427 T12465 T12466 nsubj we,performed
R9428 T12466 T12466 ROOT performed,performed
R9429 T12467 T12469 det a,immunoprecipitation
R9430 T12468 T12469 compound chromatin,immunoprecipitation
R9431 T12469 T12466 dobj immunoprecipitation,performed
R9432 T12470 T12469 punct (,immunoprecipitation
R9433 T12471 T12469 appos ChIP,immunoprecipitation
R9434 T12472 T12469 punct ),immunoprecipitation
R9435 T12473 T12466 advcl using,performed
R9436 T12474 T12476 det an,antibody
R9437 T12475 T12476 amod anti-GATA3,antibody
R9438 T12476 T12473 dobj antibody,using
R9439 T12477 T12466 cc and,performed
R9440 T12478 T12466 conj showed,performed
R9441 T12479 T12478 dobj that,showed
R9442 T12480 T12481 nummod GATA3,binds
R9443 T12481 T12479 nsubj binds,that
R9444 T12482 T12481 prep to,binds
R9445 T12483 T12486 det the,region
R9446 T12484 T12486 nummod FOXP3,region
R9447 T12485 T12486 compound promoter,region
R9448 T12486 T12482 pobj region,to
R9449 T12487 T12481 prep in,binds
R9450 T12488 T12489 amod Th2,cells
R9451 T12489 T12487 pobj cells,in
R9452 T12490 T12481 punct ",",binds
R9453 T12491 T12481 cc but,binds
R9454 T12492 T12493 neg not,in
R9455 T12493 T12497 prep in,Figure
R9456 T12494 T12495 compound iTreg,cells
R9457 T12495 T12493 pobj cells,in
R9458 T12496 T12497 punct (,Figure
R9459 T12497 T12481 appos Figure,binds
R9460 T12498 T12497 nummod 7F,Figure
R9461 T12499 T12497 punct ),Figure
R9462 T12500 T12466 punct .,performed
R9463 T12501 T12505 advcl Taken,demonstrate
R9464 T12502 T12501 advmod together,Taken
R9465 T12503 T12504 det these,data
R9466 T12504 T12505 nsubj data,demonstrate
R9467 T12505 T12505 ROOT demonstrate,demonstrate
R9468 T12506 T12514 mark that,repressing
R9469 T12507 T12508 det the,GATA3-binding
R9470 T12508 T12514 nsubj GATA3-binding,repressing
R9471 T12509 T12508 prep to,GATA3-binding
R9472 T12510 T12512 det the,promoter
R9473 T12511 T12512 nummod FOXP3,promoter
R9474 T12512 T12509 pobj promoter,to
R9475 T12513 T12514 aux is,repressing
R9476 T12514 T12505 ccomp repressing,demonstrate
R9477 T12515 T12516 nummod FOXP3,expression
R9478 T12516 T12514 dobj expression,repressing
R9479 T12517 T12505 punct .,demonstrate
R11400 T15144 T15146 det The,study
R11401 T15145 T15146 amod current,study
R11402 T15146 T15147 nsubj study,reveals
R11403 T15147 T15147 ROOT reveals,reveals
R11404 T15148 T15160 mark that,inhibited
R11405 T15149 T15150 nummod FOXP3,induction
R11406 T15150 T15160 nsubjpass induction,inhibited
R11407 T15151 T15150 punct ",",induction
R11408 T15152 T15154 det an,step
R11409 T15153 T15154 amod important,step
R11410 T15154 T15150 appos step,induction
R11411 T15155 T15154 prep in,step
R11412 T15156 T15157 compound iTreg,commitment
R11413 T15157 T15155 pobj commitment,in
R11414 T15158 T15160 punct ",",inhibited
R11415 T15159 T15160 auxpass is,inhibited
R11416 T15160 T15147 ccomp inhibited,reveals
R11417 T15161 T15160 agent by,inhibited
R11418 T15162 T15161 pobj GATA3,by
R11419 T15163 T15162 punct ",",GATA3
R11420 T15164 T15165 nsubj which,is
R11421 T15165 T15162 relcl is,GATA3
R11422 T15166 T15168 det the,regulator
R11423 T15167 T15168 amod key,regulator
R11424 T15168 T15165 attr regulator,is
R11425 T15169 T15168 prep for,regulator
R11426 T15170 T15169 pobj polarization,for
R11427 T15171 T15170 prep toward,polarization
R11428 T15172 T15173 amod Th2,cells
R11429 T15173 T15171 pobj cells,toward
R11430 T15174 T15147 punct .,reveals
R11431 T15175 T15182 prep After,become
R11432 T15176 T15175 pobj differentiation,After
R11433 T15177 T15182 punct ",",become
R11434 T15178 T15181 det the,cells
R11435 T15179 T15181 nmod effector,cells
R11436 T15180 T15181 amod Th2,cells
R11437 T15181 T15182 nsubj cells,become
R11438 T15182 T15182 ROOT become,become
R11439 T15183 T15182 acomp refractory,become
R11440 T15184 T15185 aux to,conversion
R11441 T15185 T15182 advcl conversion,become
R11442 T15186 T15185 prep into,conversion
R11443 T15187 T15190 det a,phenotype
R11444 T15188 T15190 nummod FOXP3,phenotype
R11445 T15189 T15190 compound +,phenotype
R11446 T15190 T15186 pobj phenotype,into
R11447 T15191 T15182 punct .,become
R11448 T15192 T15199 prep In,found
R11449 T15193 T15192 pobj accordance,In
R11450 T15194 T15193 prep with,accordance
R11451 T15195 T15196 amod other,studies
R11452 T15196 T15194 pobj studies,with
R11453 T15197 T15199 punct ",",found
R11454 T15198 T15199 nsubj we,found
R11455 T15199 T15199 ROOT found,found
R11456 T15200 T15205 mark that,were
R11457 T15201 T15203 nummod CD4,CD25
R11458 T15202 T15203 nummod +,CD25
R11459 T15203 T15205 nsubj CD25,were
R11460 T15204 T15203 appos cells,CD25
R11461 T15205 T15199 ccomp were,found
R11462 T15206 T15205 acomp able,were
R11463 T15207 T15208 aux to,up-regulate
R11464 T15208 T15206 xcomp up-regulate,able
R11465 T15209 T15212 nummod FOXP3,]
R11466 T15210 T15212 compound [,]
R11467 T15211 T15212 compound "12,27",]
R11468 T15212 T15208 dobj ],up-regulate
R11469 T15213 T15199 punct .,found
R11470 T15214 T15215 amod Already-committed,cells
R11471 T15215 T15224 nsubj cells,showed
R11472 T15216 T15217 amod such,as
R11473 T15217 T15215 prep as,cells
R11474 T15218 T15220 compound memory,cells
R11475 T15219 T15220 compound T,cells
R11476 T15220 T15217 pobj cells,as
R11477 T15221 T15220 cc and,cells
R11478 T15222 T15223 amod Th1,cells
R11479 T15223 T15220 conj cells,cells
R11480 T15224 T15224 ROOT showed,showed
R11481 T15225 T15226 advmod only,moderate
R11482 T15226 T15230 amod moderate,induction
R11483 T15227 T15226 cc and,moderate
R11484 T15228 T15226 conj transient,moderate
R11485 T15229 T15230 nummod FOXP3,induction
R11486 T15230 T15224 dobj induction,showed
R11487 T15231 T15230 punct ",",induction
R11488 T15232 T15233 nsubj which,is
R11489 T15233 T15230 relcl is,induction
R11490 T15234 T15233 neg not,is
R11491 T15235 T15233 acomp sufficient,is
R11492 T15236 T15237 aux to,change
R11493 T15237 T15235 xcomp change,sufficient
R11494 T15238 T15239 det the,phenotype
R11495 T15239 T15237 dobj phenotype,change
R11496 T15240 T15239 prep toward,phenotype
R11497 T15241 T15245 det a,profile
R11498 T15242 T15244 amod regulatory,cell
R11499 T15243 T15244 compound T,cell
R11500 T15244 T15245 compound cell,profile
R11501 T15245 T15240 pobj profile,toward
R11502 T15246 T15224 punct .,showed
R11503 T15247 T15255 prep In,up-regulate
R11504 T15248 T15247 pobj contrast,In
R11505 T15249 T15255 punct ",",up-regulate
R11506 T15250 T15252 amod naive,cells
R11507 T15251 T15252 compound T,cells
R11538 T15282 T15273 cc and,plays
R11539 T15283 T15284 aux may,act
R11540 T15284 T15273 conj act,plays
R11541 T15285 T15284 prep in,act
R11542 T15286 T15287 det a,way
R11543 T15287 T15285 pobj way,in
R11544 T15288 T15287 amod similar,way
R11545 T15289 T15288 prep to,similar
R11546 T15290 T15289 pobj that,to
R11547 T15291 T15290 acl known,that
R11548 T15292 T15291 prep for,known
R11549 T15293 T15296 det the,factors
R11550 T15294 T15295 compound Th1/Th2,decision
R11551 T15295 T15296 compound decision,factors
R11552 T15296 T15292 pobj factors,for
R11553 T15297 T15296 appos T-bet,factors
R11554 T15298 T15297 cc and,T-bet
R11555 T15299 T15297 conj GATA3,T-bet
R11556 T15300 T15255 punct .,up-regulate
R11618 T15362 T15361 cc and,GATA3
R11619 T15363 T15361 conj T-bet,GATA3
R11620 T15364 T15367 amod low,]
R11621 T15365 T15367 compound [,]
R11622 T15366 T15367 compound "37,38",]
R11623 T15367 T15361 appos ],GATA3
R11624 T15368 T15345 punct .,appeared
R11625 T15369 T15393 prep In,showed
R11626 T15370 T15369 pobj contrast,In
R11627 T15371 T15393 punct ",",showed
R11628 T15372 T15393 nsubj differentiating,showed
R11629 T15373 T15375 amod naive,cells
R11630 T15374 T15375 compound T,cells
R11631 T15375 T15372 dobj cells,differentiating
R11632 T15376 T15372 prep in,differentiating
R11633 T15377 T15378 det the,absence
R11634 T15378 T15376 pobj absence,in
R11697 T15441 T15442 nummod 40,]
R11698 T15442 T15442 ROOT ],]
R11699 T15443 T15442 punct ",",]
R11700 T15444 T15442 cc and,]
R11701 T15445 T15452 nsubjpass estrogens,known
R11702 T15446 T15448 nmod [,]
R11703 T15447 T15448 nummod 41,]
R11704 T15448 T15445 dobj ],estrogens
R11705 T15449 T15450 punct ",",are
R11706 T15450 T15452 auxpass are,known
R11707 T15451 T15452 advmod also,known
R11708 T15452 T15452 ROOT known,known
R11709 T15453 T15452 prep as,known
R11710 T15454 T15453 pobj inhibitors,as
R11711 T15455 T15454 prep of,inhibitors
R11712 T15456 T15457 compound GATA3,expression
R11743 T15487 T15486 cc but,IL-4
R11744 T15488 T15489 neg not,IL-13
R11745 T15489 T15494 nsubj IL-13,was
R11746 T15490 T15489 punct (,IL-13
R11747 T15491 T15492 amod unpublished,data
R11748 T15492 T15489 appos data,IL-13
R11749 T15493 T15489 punct ),IL-13
R11750 T15494 T15494 ROOT was,was
R11751 T15495 T15494 acomp able,was
R11752 T15496 T15497 aux to,inhibit
R11753 T15497 T15495 xcomp inhibit,able
R11754 T15498 T15497 dobj TGF-β,inhibit
R11755 T15499 T15494 advcl mediated,was
R11756 T15500 T15501 nummod FOXP3,induction
R11757 T15501 T15499 dobj induction,mediated
R11758 T15502 T15499 cc and,mediated
R11759 T15503 T15504 advmod therefore,prevented
R11760 T15504 T15499 conj prevented,mediated
R11761 T15505 T15504 dobj conversion,prevented
R11762 T15506 T15504 prep into,prevented
R11763 T15507 T15509 det the,phenotype
R11764 T15508 T15509 amod regulatory,phenotype
R11765 T15509 T15506 pobj phenotype,into
R11766 T15510 T15494 punct .,was
R11767 T15511 T15512 aux To,prove
R11768 T15512 T15528 advcl prove,treated
R11769 T15513 T15515 det the,effect
R11770 T15514 T15515 amod inhibitory,effect
R11771 T15515 T15512 dobj effect,prove
R11772 T15516 T15515 prep of,effect
R11773 T15517 T15516 pobj IL-4,of
R11774 T15518 T15517 prep on,IL-4
R11775 T15519 T15523 amod inducible,commitment
R11776 T15520 T15519 cc or,inducible
R11777 T15521 T15519 conj natural,inducible
R11778 T15522 T15523 compound Treg,commitment
R11779 T15523 T15518 pobj commitment,on
R11780 T15524 T15523 prep in,commitment
R11781 T15525 T15524 pobj vivo,in
R11782 T15526 T15528 punct ",",treated
R11783 T15527 T15528 nsubj we,treated
R11784 T15528 T15528 ROOT treated,treated
R11785 T15529 T15528 dobj mice,treated
R11786 T15530 T15528 prep with,treated
R11787 T15531 T15530 pobj IL-4,with
R11788 T15532 T15531 cc and,IL-4
R11789 T15533 T15531 conj anti-IL-4,IL-4
R11790 T15534 T15528 punct .,treated
R11792 T15535 T15538 nsubjpass This,shown
R11794 T15536 T15538 aux has,shown
R11798 T15537 T15538 auxpass been,shown
R11803 T15538 T15538 ROOT shown,shown
R11805 T15539 T15540 aux to,increase
R11808 T15540 T15538 xcomp increase,shown
R11812 T15541 T15542 det the,effect
R11819 T15542 T15540 dobj effect,increase
R11823 T15543 T15542 prep of,effect
R11827 T15544 T15545 det the,cytokine
R11830 T15545 T15543 pobj cytokine,of
R11836 T15546 T15545 prep in,cytokine
R11840 T15547 T15546 pobj vivo,in
R11845 T15548 T15550 nmod [,]
R11846 T15549 T15550 nummod 19,]
R11849 T15550 T15550 ROOT ],]
R11854 T15551 T15538 punct .,shown
R11856 T15552 T15559 advmod Only,resulted
R11857 T15553 T15558 det the,complex
R11860 T15554 T15556 compound IL-4,IL-4
R11863 T15555 T15556 compound /,IL-4
R11872 T15556 T15558 compound IL-4,complex
R11877 T15557 T15558 compound mAb,complex
R11882 T15558 T15559 nsubj complex,resulted
R11886 T15676 T15675 appos producing,IL-17
R11887 T15559 T15559 ROOT resulted,resulted
R11888 T15677 T15678 compound T,cells
R11889 T15678 T15676 dobj cells,producing
R11890 T15679 T15680 punct (,Th17
R11891 T15680 T15678 appos Th17,cells
R11892 T15560 T15559 prep in,resulted
R11893 T15681 T15675 punct ),IL-17
R11894 T15682 T15684 compound [,]
R11895 T15683 T15684 compound "46,47",]
R11896 T15561 T15562 det a,decrease
R11897 T15684 T15684 ROOT ],]
R11898 T15685 T15684 punct .,]
R11899 T15686 T15687 amod Similar,effects
R11900 T15562 T15560 pobj decrease,in
R11901 T15687 T15691 nsubjpass effects,described
R11902 T15688 T15691 aux have,described
R11903 T15689 T15691 auxpass been,described
R11904 T15563 T15562 prep of,decrease
R11905 T15690 T15691 advmod recently,described
R11906 T15691 T15691 ROOT described,described
R11907 T15692 T15691 prep for,described
R11908 T15564 T15565 det the,amount
R11909 T15693 T15692 pobj IL-6,for
R11910 T15565 T15563 pobj amount,of
R11913 T15566 T15565 prep of,amount
R11955 T15725 T15724 prep into,polarization
R11957 T15726 T15727 compound iTreg,cells
R11958 T15727 T15725 pobj cells,into
R11960 T15728 T15730 auxpass is,regulated
R11961 T15729 T15730 advmod negatively,regulated
R11962 T15730 T15730 ROOT regulated,regulated
R11964 T15731 T15730 agent by,regulated
R11965 T15732 T15735 det the,IL-4
R11966 T15733 T15734 compound effector,cytokines
R11967 T15734 T15735 compound cytokines,IL-4
R11968 T15735 T15731 pobj IL-4,by
R11969 T15736 T15735 cc and,IL-4
R11971 T15737 T15735 conj IL-6,IL-4
R11972 T15738 T15730 punct .,regulated
R11973 T15739 T15743 nsubjpass IL-4,shown
R11975 T15740 T15743 aux has,shown
R11976 T15741 T15743 auxpass been,shown
R11977 T15742 T15743 advmod previously,shown
R11979 T15743 T15743 ROOT shown,shown
R11980 T15744 T15745 aux to,induce
R11981 T15745 T15743 xcomp induce,shown
R11983 T15746 T15747 det the,generation
R11984 T15747 T15745 dobj generation,induce
R11985 T15748 T15747 prep of,generation
R11987 T15749 T15752 nummod FOXP3,cells
R11988 T15750 T15752 nummod +,cells
R11989 T15751 T15752 compound Treg,cells
R11991 T15752 T15748 pobj cells,of
R11992 T15753 T15745 prep out,induce
R11993 T15754 T15753 prep of,out
R11994 T15588 T15591 nsubj IL-4,interfering
R11995 T15755 T15757 compound CD4,CD25
R11996 T15756 T15757 compound +,CD25
R11997 T15757 T15754 pobj CD25,of
R11998 T15758 T15760 nmod [,]
R11999 T15589 T15591 aux is,interfering
R12000 T15759 T15760 nummod 50,]
R12001 T15760 T15760 ROOT ],]
R12002 T15761 T15743 punct .,shown
R12003 T15762 T15771 prep In,were
R12004 T15590 T15591 advmod only,interfering
R12005 T15763 T15764 det those,experiments
R12006 T15764 T15762 pobj experiments,In
R12007 T15765 T15771 punct ",",were
R12008 T15766 T15767 det the,concentrations
R12009 T15591 T15586 ccomp interfering,suggest
R12010 T15767 T15771 nsubj concentrations,were
R12011 T15768 T15767 prep of,concentrations
R12012 T15769 T15768 pobj IL-4,of
R12013 T15592 T15591 prep with,interfering
R12014 T15770 T15767 acl used,concentrations
R12015 T15771 T15771 ROOT were,were
R12016 T15772 T15771 acomp low,were
R12017 T15593 T15594 det the,differentiation
R12018 T15773 T15771 punct ",",were
R12019 T15594 T15592 pobj differentiation,with
R12020 T15774 T15771 cc and,were
R12021 T15775 T15778 mark as,observed
R12022 T15776 T15778 nsubj we,observed
R12023 T15595 T15594 prep of,differentiation
R12024 T15777 T15778 advmod also,observed
R12025 T15778 T15771 conj observed,were
R12026 T15779 T15778 punct ",",observed
R12027 T15780 T15778 npadvmod IL-4,observed
R12028 T15596 T15598 amod naive,cells
R12029 T15781 T15778 prep at,observed
R12030 T15782 T15783 amod low,concentration
R12031 T15783 T15781 pobj concentration,at
R12032 T15597 T15598 compound T,cells
R12033 T15784 T15785 advmod slightly,enhanced
R12034 T15785 T15787 amod enhanced,expression
R12035 T15786 T15787 nummod FOXP3,expression
R12036 T15598 T15595 pobj cells,of
R12037 T15787 T15778 npadvmod expression,observed
R12038 T15788 T15771 punct .,were
R12039 T15599 T15594 prep into,differentiation
R12040 T15789 T15793 advmod Importantly,were
R12041 T15790 T15793 punct ",",were
R12042 T15791 T15792 det these,concentrations
R12043 T15600 T15601 compound iTreg,cells
R12044 T15792 T15793 nsubj concentrations,were
R12045 T15793 T15793 ROOT were,were
R12046 T15794 T15793 neg not,were
R12047 T15601 T15599 pobj cells,into
R12048 T15795 T15793 acomp sufficient,were
R12049 T15796 T15797 aux to,induce
R12050 T15797 T15795 xcomp induce,sufficient
R12051 T15602 T15586 punct .,suggest
R12052 T15798 T15799 compound GATA3,expression
R12053 T15603 T15630 cc But,exclude
R12054 T15604 T15630 prep since,exclude
R12056 T15799 T15797 dobj expression,induce
R12057 T15800 T15793 punct .,were
R12058 T15801 T15803 nsubj IL-4,favor
R12059 T15802 T15803 aux may,favor
R12061 T15803 T15803 ROOT favor,favor
R12063 T15804 T15803 dobj proliferation,favor
R12064 T15805 T15804 prep of,proliferation
R12066 T15806 T15807 compound nTreg,cells
R12067 T15807 T15805 pobj cells,of
R12068 T15808 T15813 nsubj [,regulate
R12069 T15809 T15810 nummod 47,]
R12070 T15810 T15813 nsubj ],regulate
R12071 T15811 T15810 cc or,]
R12073 T15812 T15813 advmod directly,regulate
R12074 T15813 T15803 conj regulate,favor
R12075 T15814 T15815 nummod FOXP3,expression
R12077 T15815 T15813 dobj expression,regulate
R12078 T15816 T15813 prep in,regulate
R12079 T15817 T15819 det a,fashion
R12081 T15818 T15819 amod STAT-dependent,fashion
R12082 T15819 T15816 pobj fashion,in
R12083 T15820 T15822 nmod [,]
R12085 T15821 T15822 nummod 51,]
R12086 T15822 T15813 npadvmod ],regulate
R12087 T15823 T15813 punct .,regulate
R12089 T15824 T15829 mark Since,reduce
R12090 T15825 T15829 nsubj IL-13,reduce
R12091 T15826 T15829 aux does,reduce
R12093 T15827 T15829 neg not,reduce
R12094 T15828 T15829 advmod effectively,reduce
R12095 T15829 T15838 advcl reduce,hypothesized
R12097 T15830 T15829 dobj FOXP3,reduce
R12098 T15831 T15829 cc and,reduce
R12099 T15832 T15829 conj fails,reduce
R12101 T15833 T15834 aux to,induce
R12102 T15834 T15832 xcomp induce,fails
R12103 T15835 T15834 dobj GATA3,induce
R12104 T15836 T15838 punct ",",hypothesized
R12106 T15837 T15838 nsubj we,hypothesized
R12107 T15838 T15838 ROOT hypothesized,hypothesized
R12108 T15839 T15848 mark that,mediated
R12110 T15840 T15841 det the,IL-4
R12111 T15841 T15848 nsubjpass IL-4,mediated
R12112 T15842 T15843 amod dependent,inhibition
R12114 T15843 T15841 appos inhibition,IL-4
R12115 T15844 T15843 prep of,inhibition
R12116 T15845 T15844 pobj FOXP3,of
R12117 T15846 T15848 aux could,mediated
R12118 T15847 T15848 auxpass be,mediated
R12119 T15848 T15838 ccomp mediated,hypothesized
R12121 T15849 T15848 agent by,mediated
R12168 T15917 T15919 amod naive,cells
R12169 T15882 T15881 cc and,GATA3
R12170 T15883 T15881 conj FOXP3,GATA3
R12171 T15884 T15885 compound transcription,factors
R12172 T15918 T15919 compound T,cells
R12173 T15885 T15879 conj factors,mechanism
R12174 T15886 T15876 prep in,suggested
R12175 T15919 T15916 pobj cells,into
R12176 T15920 T15920 ROOT inhibited,inhibited
R12177 T15921 T15922 nummod FOXP3,induction
R12178 T15922 T15920 dobj induction,inhibited
R12179 T15923 T15922 prep in,induction
R12183 T15924 T15930 amod human,cells
R12187 T15925 T15924 punct ",",human
R12191 T15926 T15924 conj differentiating,human
R12195 T15927 T15930 punct ",",cells
R12196 T15928 T15930 amod naive,cells
R12197 T15929 T15930 compound T,cells
R12200 T15930 T15923 pobj cells,in
R12203 T15931 T15920 punct .,inhibited
R12207 T15932 T15934 det This,effect
R12211 T15933 T15934 amod inhibitory,effect
R12214 T15934 T15939 nsubjpass effect,confirmed
R12215 T15935 T15934 prep of,effect
R12216 T16007 T16008 advmod however,displayed
R12217 T15936 T15935 pobj GATA3,of
R12218 T16008 T16004 relcl displayed,cells
R12219 T16009 T16011 det a,%
R12220 T16010 T16011 nummod 10,%
R12221 T16011 T16008 dobj %,displayed
R12222 T16012 T16013 nummod 25,%
R12223 T16013 T16014 npadvmod %,lower
R12224 T16014 T16015 amod lower,frequency
R12225 T15937 T15939 auxpass was,confirmed
R12226 T16015 T16008 npadvmod frequency,displayed
R12227 T16016 T16008 prep compared,displayed
R12228 T16017 T16016 prep to,compared
R12229 T15938 T15939 advmod further,confirmed
R12230 T16018 T16021 amod wild-type,mice
R12231 T16019 T16021 nmod DO11,mice
R12232 T16020 T16021 nummod .10,mice
R12233 T15939 T15939 ROOT confirmed,confirmed
R12234 T16021 T16017 pobj mice,to
R12235 T16022 T16000 punct .,have
R12236 T16023 T16025 advmod Thus,restrains
R12237 T15940 T15939 prep in,confirmed
R12238 T16024 T16025 nsubj GATA3,restrains
R12239 T16025 T16025 ROOT restrains,restrains
R12240 T16026 T16027 det the,development
R12241 T15941 T15943 nmod BALB/c,mice
R12242 T16027 T16025 dobj development,restrains
R12243 T16028 T16027 prep of,development
R12244 T16029 T16031 amod certain,subsets
R12245 T15942 T15943 amod transgenic,mice
R12246 T16030 T16031 compound Treg,subsets
R12247 T16031 T16028 pobj subsets,of
R12248 T15943 T15940 pobj mice,in
R12249 T16032 T16031 punct ",",subsets
R12250 T16033 T16038 advmod presumably,population
R12251 T16034 T16038 det the,population
R12252 T15944 T15939 punct ",",confirmed
R12253 T16035 T16038 amod inducible,population
R12254 T16036 T16038 punct ",",population
R12255 T16037 T16038 amod peripheral,population
R12256 T15945 T15939 advcl expressing,confirmed
R12257 T16038 T16031 conj population,subsets
R12258 T16039 T16038 cc and,population
R12259 T16040 T16041 neg not,those
R12260 T15946 T15945 dobj GATA3,expressing
R12261 T15947 T15945 prep in,expressing
R12262 T16041 T16038 conj those,population
R12263 T16042 T16041 prep of,those
R12264 T15948 T15949 compound T,cells
R12265 T16043 T16044 amod thymic,origin
R12266 T16044 T16042 pobj origin,of
R12267 T15949 T15947 pobj cells,in
R12268 T16045 T16025 punct .,restrains
R12269 T16046 T16048 compound Thymic,cells
R12270 T16047 T16048 compound T,cells
R12271 T16048 T16049 nsubj cells,undergo
R12272 T16049 T16049 ROOT undergo,undergo
R12273 T15950 T15951 punct (,DO11
R12274 T16050 T16053 det a,process
R12275 T16051 T16053 amod different,process
R12276 T15951 T15956 nummod DO11,mice
R12277 T16052 T16053 compound maturation,process
R12278 T15952 T15956 nummod .10,mice
R12279 T16053 T16049 dobj process,undergo
R12280 T16054 T16053 punct ",",process
R12281 T16055 T16057 nsubj which,explain
R12282 T15953 T15956 punct :,mice
R12283 T16056 T16057 aux may,explain
R12284 T16057 T16053 relcl explain,process
R12285 T15954 T15956 amod CD2-GATA3,mice
R12286 T16058 T16059 det the,insensitivity
R12287 T16059 T16057 dobj insensitivity,explain
R12288 T16060 T16059 prep of,insensitivity
R12289 T15955 T15956 amod transgenic,mice
R12290 T16061 T16060 pobj nTreg,of
R12291 T16062 T16059 prep to,insensitivity
R12292 T16063 T16065 compound GATA3,[
R12293 T16064 T16065 compound overexpression,[
R12294 T15956 T15945 conj mice,expressing
R12295 T16065 T16062 pobj [,to
R12296 T16066 T16067 nummod 52,]
R12297 T16067 T16065 appos ],[
R12298 T15957 T15939 punct ),confirmed
R12299 T16068 T16049 punct .,undergo
R12300 T16069 T16069 ROOT In,In
R12301 T16070 T16069 pobj contrast,In
R12302 T15958 T15939 punct .,confirmed
R12303 T16071 T16070 prep to,contrast
R12304 T16072 T16073 det the,Th2-differentiating
R12305 T15959 T15976 prep In,failed
R12306 T16073 T16071 pobj Th2-differentiating,to
R12307 T16074 T16073 cc and,Th2-differentiating
R12308 T16075 T16076 amod iTreg-inhibiting,function
R12309 T16076 T16073 conj function,Th2-differentiating
R12310 T15960 T15959 pobj line,In
R12311 T16077 T16076 prep of,function
R12312 T16078 T16077 pobj GATA3,of
R12313 T16079 T16076 prep in,function
R12315 T16080 T16082 amod peripheral,cells
R12316 T16081 T16082 compound T,cells
R12317 T16082 T16079 pobj cells,in
R12318 T16083 T16073 punct ",",Th2-differentiating
R12319 T16084 T16085 amod GATA3,acts
R12320 T16085 T16069 pobj acts,In
R12321 T16086 T16085 prep in,acts
R12322 T16087 T16088 det the,thymus
R12323 T16088 T16086 pobj thymus,in
R12325 T16089 T16085 advmod together,acts
R12326 T16090 T16089 prep with,together
R12328 T16091 T16093 amod other,factors
R12331 T16092 T16093 compound transcription,factors
R12332 T16093 T16090 pobj factors,with
R12333 T16094 T16095 amod such,as
R12335 T16095 T16093 prep as,factors
R12336 T16096 T16097 det the,Repressor
R12338 T16097 T16095 pobj Repressor,as
R12339 T16098 T16097 prep of,Repressor
R12340 T16099 T16098 pobj GATA3,of
R12341 T16100 T16099 punct (,GATA3
R12343 T16101 T16099 appos ROG,GATA3
R12344 T16102 T16099 punct ),GATA3
R12345 T16103 T16097 prep in,Repressor
R12346 T15969 T15970 compound T,cells
R12347 T16104 T16106 det the,process
R12348 T16105 T16106 compound differentiation,process
R12349 T16106 T16103 pobj process,in
R12350 T15970 T15967 pobj cells,in
R12351 T16107 T16106 prep toward,process
R12352 T16108 T16109 compound CD8,cells
R12353 T16109 T16107 pobj cells,toward
R12354 T15971 T15976 punct ",",failed
R12355 T16110 T16112 compound [,]
R12356 T16111 T16112 compound "53,54",]
R12357 T16112 T16097 conj ],Repressor
R12358 T16113 T16112 cc or,]
R12359 T15972 T15976 nsubj cells,failed
R12360 T16114 T16112 conj participates,]
R12361 T16115 T16114 prep in,participates
R12362 T16116 T16119 amod complex,network
R12363 T15973 T15972 prep of,cells
R12364 T16117 T16119 amod transcriptional,network
R12365 T16118 T16119 compound feedback,network
R12366 T15974 T15975 det these,mice
R12367 T16119 T16115 pobj network,in
R12368 T15975 T15973 pobj mice,of
R12369 T16120 T16121 aux to,regulate
R12370 T16121 T16114 advcl regulate,participates
R12371 T16122 T16123 amod sympathoadrenal,differentiation
R12372 T16123 T16121 dobj differentiation,regulate
R12373 T16124 T16126 nmod [,]
R12374 T16125 T16126 nummod 55,]
R12375 T16126 T16123 appos ],differentiation
R12376 T15976 T15976 ROOT failed,failed
R12377 T16127 T16085 punct .,acts
R12378 T16128 T16134 advmod Therefore,appears
R12379 T16129 T16134 punct ",",appears
R12380 T16130 T16131 det the,role
R12381 T15977 T15978 aux to,induce
R12382 T16131 T16134 nsubj role,appears
R12383 T16132 T16131 prep of,role
R12384 T16133 T16132 pobj GATA3,of
R12385 T16134 T16134 ROOT appears,appears
R12386 T16135 T16136 aux to,be
R12387 T15978 T15976 xcomp induce,failed
R12388 T16136 T16134 xcomp be,appears
R12389 T16137 T16138 compound tissue,specific
R12390 T16138 T16136 acomp specific,be
R12391 T15979 T15980 nummod FOXP3,expression
R12392 T16139 T16134 cc and,appears
R12393 T16140 T16143 aux can,generalized
R12394 T15980 T15978 dobj expression,induce
R12395 T16141 T16143 neg not,generalized
R12396 T15981 T15980 prep upon,expression
R12397 T16142 T16143 auxpass be,generalized
R12398 T16143 T16134 conj generalized,appears
R12399 T15982 T15981 pobj exposure,upon
R12400 T16144 T16134 punct .,appears
R12401 T16145 T16146 poss Our,study
R12402 T16146 T16147 nsubj study,demonstrates
R12403 T16189 T16190 nummod 24,]
R12404 T16147 T16147 ROOT demonstrates,demonstrates
R12405 T16148 T16150 mark that,repressed
R12406 T16149 T16150 nsubj GATA3,repressed
R12407 T16150 T16147 ccomp repressed,demonstrates
R12408 T16151 T16152 nummod FOXP3,expression
R12409 T16152 T16150 dobj expression,repressed
R12410 T16153 T16150 advmod directly,repressed
R12411 T16190 T16184 pobj ],as
R12412 T16154 T16150 prep by,repressed
R12413 T16155 T16154 pobj binding,by
R12414 T16156 T16155 prep to,binding
R12415 T16191 T16166 punct .,located
R12416 T16157 T16160 det the,region
R12417 T16158 T16160 nummod FOXP3,region
R12418 T16159 T16160 compound promoter,region
R12419 T16160 T16156 pobj region,to
R12420 T16161 T16147 punct .,demonstrates
R12421 T16162 T16164 det A,GATA-site
R12422 T16163 T16164 amod palindromic,GATA-site
R12423 T16192 T16193 amod Site-specific,mutation
R12424 T16164 T16166 nsubjpass GATA-site,located
R12425 T16165 T16166 auxpass is,located
R12426 T16166 T16166 ROOT located,located
R12427 T16193 T16197 nsubj mutation,increased
R12428 T16167 T16168 nummod 303,bp
R12429 T16168 T16169 compound bp,upstream
R12430 T16169 T16166 advmod upstream,located
R12431 T16170 T16169 prep of,upstream
R12432 T16194 T16193 prep of,mutation
R12433 T16171 T16172 det the,TSS
R12434 T16172 T16170 pobj TSS,of
R12435 T16173 T16166 prep in,located
R12436 T16195 T16196 det this,site
R12437 T16174 T16177 det a,region
R12438 T16175 T16176 advmod highly,conserved
R12439 T16176 T16177 amod conserved,region
R12440 T16196 T16194 pobj site,of
R12441 T16177 T16173 pobj region,in
R12442 T16178 T16177 punct ",",region
R12443 T16179 T16183 dobj which,identified
R12444 T16197 T16197 ROOT increased,increased
R12445 T16180 T16183 nsubj we,identified
R12446 T16181 T16183 aux have,identified
R12447 T16182 T16183 advmod previously,identified
R12449 T16183 T16177 relcl identified,region
R12450 T16184 T16183 prep as,identified
R12451 T16185 T16190 det the,]
R12452 T16186 T16190 nummod FOXP3,]
R12454 T16187 T16188 compound promoter,[
R12455 T16188 T16190 nmod [,]
R12461 T16286 T16278 punct ",",induce
R12463 T16287 T16278 prep by,induce
R12464 T16288 T16289 advmod directly,transactivating
R12465 T16289 T16287 pcomp transactivating,by
R12467 T16290 T16289 dobj promoters,transactivating
R12468 T16291 T16293 nmod [,]
R12469 T16292 T16293 nummod 36,]
R12470 T16293 T16289 dobj ],transactivating
R12471 T16294 T16293 punct ",",]
R12472 T16295 T16289 cc or,transactivating
R12474 T16296 T16278 punct ",",induce
R12475 T16297 T16298 mark as,shown
R12476 T16298 T16278 advcl shown,induce
R12477 T16299 T16298 prep in,shown
R12479 T16300 T16302 det the,study
R12480 T16301 T16302 amod current,study
R12481 T16302 T16299 pobj study,in
R12483 T16303 T16304 punct ",",acts
R12484 T16304 T16304 ROOT acts,acts
R12485 T16305 T16304 prep as,acts
R12487 T16306 T16307 det a,repressor
R12488 T16307 T16305 pobj repressor,as
R12489 T16308 T16307 prep of,repressor
R12491 T16309 T16310 compound gene,expression
R12492 T16310 T16308 pobj expression,of
R12493 T16311 T16312 compound [,"21,57"
R12495 T16312 T16307 appos "21,57",repressor
R12496 T16313 T16314 nummod 59,]
R12497 T16314 T16307 appos ],repressor
R12499 T16315 T16304 punct .,acts
R12500 T16316 T16323 advmod Therefore,required
R12501 T16317 T16323 nsubjpass keeping,required
R12503 T16318 T16319 amod GATA3,expression
R12504 T16319 T16317 dobj expression,keeping
R12505 T16320 T16317 oprd low,keeping
R12507 T16321 T16323 aux might,required
R12508 T16322 T16323 auxpass be,required
R12509 T16323 T16323 ROOT required,required
R12511 T16324 T16325 aux to,induce
R12512 T16325 T16323 xcomp induce,required
R12513 T16326 T16331 amod efficient,generation
R12514 T16327 T16331 nummod FOXP3,generation
R12516 T16328 T16331 nummod +,generation
R12517 T16329 T16331 compound iTreg,generation
R12518 T16330 T16331 compound cell,generation
R12519 T16331 T16325 dobj generation,induce
R12520 T16332 T16323 punct .,required
R12521 T16333 T16335 det The,interactions
R12523 T16334 T16335 amod molecular,interactions
R12524 T16335 T16343 nsubjpass interactions,identified
R12525 T16336 T16335 acl enabling,interactions
R12526 T16337 T16336 dobj GATA3,enabling
R12528 T16338 T16339 aux to,inhibit
R12529 T16339 T16336 xcomp inhibit,enabling
R12530 T16340 T16343 nsubjpass FOXP3,identified
R12532 T16341 T16343 auxpass are,identified
R12533 T16342 T16343 neg not,identified
R12534 T16343 T16343 ROOT identified,identified
R12536 T16344 T16343 advmod yet,identified
R12537 T16345 T16343 punct ",",identified
R12538 T16346 T16343 cc but,identified
R12540 T16347 T16349 det the,site
R12541 T16348 T16349 compound GATA-binding,site
R12542 T16349 T16351 nsubjpass site,located
R12544 T16350 T16351 auxpass is,located
R12545 T16351 T16343 conj located,identified
R12546 T16352 T16351 oprd adjacent,located
R12548 T16353 T16352 prep to,adjacent
R12549 T16354 T16353 amod positive,to
R12551 T16355 T16351 punct ",",located
R12553 T16356 T16351 advcl inducing,located
R12554 T16357 T16356 dobj sites,inducing
R12555 T16358 T16357 punct ",",sites
R12557 T16359 T16357 acl composed,sites
R12558 T16360 T16359 prep of,composed
R12560 T16361 T16362 amod AP-1-NFATc2,sites
R12561 T16362 T16360 pobj sites,of
R12562 T16363 T16365 nmod [,]
R12564 T16364 T16365 nummod 24,]
R12565 T16365 T16370 nsubj ],compete
R12566 T16366 T16365 punct ",",]
R12568 T16367 T16365 cc and,]
R12569 T16368 T16365 conj GATA3,]
R12570 T16369 T16370 aux may,compete
R12571 T16370 T16343 conj compete,identified
R12572 T16371 T16370 prep with,compete
R12573 T16372 T16373 det the,binding
R12574 T16373 T16371 pobj binding,with
R12576 T16374 T16373 prep of,binding
R12577 T16375 T16377 compound AP-1,NFAT
R12578 T16376 T16377 compound /,NFAT
R12579 T16377 T16374 pobj NFAT,of
R12580 T16232 T16229 pobj cells,in
R12581 T16378 T16373 prep to,binding
R12582 T16379 T16380 det the,promoter
R12583 T16380 T16378 pobj promoter,to
R12584 T16233 T16232 punct ",",cells
R12585 T16381 T16380 punct (,promoter
R12586 T16382 T16383 amod unpublished,observations
R12587 T16234 T16237 nsubj which,express
R12588 T16235 T16237 aux do,express
R12589 T16236 T16237 neg not,express
R12590 T16383 T16380 appos observations,promoter
R12591 T16237 T16232 relcl express,cells
R12592 T16384 T16380 punct ),promoter
R12593 T16385 T16370 punct .,compete
R12594 T16386 T16390 prep In,demonstrated
R12595 T16238 T16237 dobj GATA3,express
R12596 T16387 T16386 pobj summary,In
R12597 T16388 T16390 punct ",",demonstrated
R12598 T16239 T16197 punct .,increased
R12599 T16389 T16390 nsubj we,demonstrated
R12600 T16390 T16390 ROOT demonstrated,demonstrated
R12601 T16391 T16395 mark that,regulated
R12602 T16240 T16243 det This,element
R12603 T16392 T16395 nsubjpass FOXP3,regulated
R12604 T16393 T16395 auxpass is,regulated
R12605 T16394 T16395 advmod negatively,regulated
R12606 T16241 T16243 amod palindromic,element
R12607 T16395 T16390 ccomp regulated,demonstrated
R12608 T16396 T16395 agent by,regulated
R12609 T16397 T16396 pobj cytokines,by
R12611 T16398 T16399 amod such,as
R12612 T16399 T16397 prep as,cytokines
R12613 T16400 T16399 pobj IL-4,as
R12615 T16401 T16390 punct .,demonstrated
R12616 T16402 T16403 nummod GATA3,acts
R12617 T16403 T16403 ROOT acts,acts
R12618 T16404 T16403 prep as,acts
R12620 T16405 T16406 det an,inhibitor
R12622 T16406 T16404 pobj inhibitor,as
R12623 T16407 T16406 prep of,inhibitor
R12625 T16408 T16409 nummod FOXP3,expression
R12626 T16409 T16407 pobj expression,of
R12627 T16410 T16406 prep in,inhibitor
R12629 T16411 T16414 amod early,differentiation
R12630 T16412 T16414 compound T,differentiation
R12631 T16413 T16414 compound cell,differentiation
R12632 T16414 T16410 pobj differentiation,in
R12634 T16415 T16406 punct ",",inhibitor
R12635 T16416 T16418 advmod as,as
R12637 T16417 T16418 advmod well,as
R12638 T16418 T16404 cc as,as
R12639 T16419 T16404 prep in,as
R12640 T16420 T16422 amod differentiated,cells
R12642 T16421 T16422 amod Th2,cells
R12643 T16422 T16419 pobj cells,in
R12644 T16423 T16403 prep by,acts
R12646 T16424 T16425 advmod directly,binding
R12647 T16425 T16403 advcl binding,acts
R12648 T16426 T16425 cc and,binding
R12650 T16427 T16425 conj repressing,binding
R12651 T16428 T16430 det the,promoter
R12652 T16429 T16430 nummod FOXP3,promoter
R12653 T16430 T16427 dobj promoter,repressing
R12655 T16431 T16403 punct .,acts
R12656 T16432 T16434 nsubj We,describe
R12657 T16433 T16434 advmod therefore,describe
R12660 T16434 T16434 ROOT describe,describe
R12661 T16435 T16437 det a,mechanism
R12662 T16436 T16437 amod new,mechanism
R12664 T16437 T16434 dobj mechanism,describe
R12665 T16438 T16437 prep of,mechanism
R12666 T16439 T16441 advmod how,avoids
R12668 T16440 T16441 nsubj Il-4,avoids
R12669 T16441 T16438 pcomp avoids,of
R12670 T16442 T16443 compound tolerance,induction
R12671 T16443 T16441 dobj induction,avoids
R12672 T16444 T16434 prep by,describe
R12673 T16445 T16444 pcomp repressing,by
R12675 T16446 T16447 nummod FOXP3,expression
R12676 T16447 T16445 dobj expression,repressing
R12677 T16448 T16434 punct .,describe
R12678 T16449 T16450 det These,findings
R12680 T16450 T16452 nsubj findings,give
R12681 T16451 T16452 aux will,give
R12683 T16452 T16452 ROOT give,give
R12684 T16453 T16454 amod new,perspectives
R12685 T16454 T16452 dobj perspectives,give
R12686 T16455 T16454 prep toward,perspectives
R12688 T16456 T16455 pcomp understanding,toward
R12689 T16457 T16458 amod molecular,mechanisms
R12690 T16458 T16456 dobj mechanisms,understanding
R12692 T16459 T16458 prep of,mechanisms
R12693 T16460 T16461 compound iTreg,induction
R12695 T16461 T16459 pobj induction,of
R12696 T16462 T16455 cc and,toward
R12697 T16463 T16464 amod thus,pathways
R12699 T16464 T16455 pobj pathways,toward
R12700 T16465 T16464 prep of,pathways
R12701 T16466 T16468 amod peripheral,induction
R12703 T16467 T16468 compound tolerance,induction
R12704 T16468 T16465 pobj induction,of
R12705 T16469 T16464 punct ",",pathways
R12707 T16470 T16471 advmod particularly,in
R12708 T16471 T16452 prep in,give
R12709 T16472 T16471 pobj allergy,in
R12711 T16473 T16472 cc and,allergy
R12712 T16474 T16472 conj asthma,allergy
R12713 T16475 T16452 punct .,give
R12726 T16277 T16278 aux can,induce
R12728 T16278 T16274 ccomp induce,known
R12730 T16279 T16278 dobj transcription,induce
R12733 T16280 T16278 prep by,induce
R12740 T16281 T16280 pobj chromatin,by
R12741 T16282 T16285 nmod remodeling,]
R12742 T16283 T16282 dobj [,remodeling
R12746 T16284 T16285 nummod 56,]
R12750 T16285 T16278 npadvmod ],induce
R13523 T17891 T17891 ROOT Mice,Mice
R13598 T17966 T17965 prep for,background
R13599 T17967 T17968 det a,minimum
R13600 T17968 T17966 pobj minimum,for
R13601 T17969 T17968 prep of,minimum
R13602 T17970 T17971 nummod eight,generations
R13603 T17971 T17969 pobj generations,of
R13604 T17972 T17961 cc and,backcrossed
R13605 T17973 T17961 conj used,backcrossed
R13606 T17974 T17973 prep at,used
R13607 T17975 T17976 det an,age
R13608 T17976 T17974 pobj age,at
R13609 T17977 T17976 prep of,age
R13610 T17978 T17977 pobj 8,of
R13611 T17979 T17980 nummod 12,wk
R13612 T17980 T17978 appos wk,8
R13613 T17981 T17961 punct .,backcrossed
R13614 T17982 T17984 nsubjpass Mice,housed
R13615 T17983 T17984 auxpass were,housed
R13616 T17984 T17984 ROOT housed,housed
R13617 T17985 T17984 prep under,housed
R13618 T17986 T17988 amod specific,conditions
R13619 T17987 T17988 amod pathogen-free,conditions
R13620 T17988 T17985 pobj conditions,under
R13621 T17989 T17988 cc and,conditions
R13622 T17990 T17992 det all,studies
R13623 T17991 T17992 compound animal,studies
R13624 T17992 T17994 nsubjpass studies,performed
R13625 T17993 T17994 auxpass were,performed
R13626 T17994 T17984 conj performed,housed
R13627 T17995 T17994 prep according,performed
R13628 T17996 T17995 prep to,according
R13629 T17997 T18000 amod institutional,guidelines
R13630 T17998 T17997 cc and,institutional
R13631 T17999 T17997 conj state,institutional
R13632 T18000 T17996 pobj guidelines,to
R13633 T18001 T17994 punct .,performed
R13728 T18166 T18166 ROOT Isolation,Isolation
R13729 T18167 T18166 prep of,Isolation
R13730 T18168 T18171 nummod CD4,cells
R13731 T18169 T18171 nummod +,cells
R13732 T18170 T18171 compound T,cells
R13733 T18171 T18167 pobj cells,of
R13734 T18172 T18166 punct .,Isolation
R13735 T18173 T18178 nsubjpass CD4,isolated
R13736 T18174 T18176 nummod +,cells
R13737 T18175 T18176 compound T,cells
R13738 T18176 T18177 nsubj cells,were
R13739 T18177 T18178 auxpass were,isolated
R13740 T18178 T18178 ROOT isolated,isolated
R13741 T18179 T18178 prep from,isolated
R13742 T18180 T18179 pobj blood,from
R13743 T18181 T18180 prep of,blood
R13744 T18182 T18184 amod healthy,volunteers
R13745 T18183 T18184 amod human,volunteers
R13746 T18184 T18181 pobj volunteers,of
R13747 T18185 T18184 acl using,volunteers
R13748 T18186 T18189 det the,beads
R13749 T18187 T18189 amod anti-CD4,beads
R13750 T18188 T18189 amod magnetic,beads
R13751 T18189 T18185 dobj beads,using
R13752 T18190 T18189 punct (,beads
R13753 T18191 T18189 appos Dynal,beads
R13754 T18192 T18191 punct ",",Dynal
R13755 T18193 T18191 conj Hamburg,Dynal
R13756 T18194 T18193 punct ",",Hamburg
R13757 T18195 T18193 appos Germany,Hamburg
R13758 T18196 T18193 punct ),Hamburg
R13759 T18197 T18199 mark as,described
R13760 T18198 T18199 advmod previously,described
R13761 T18199 T18178 advcl described,isolated
R13762 T18200 T18202 nmod [,]
R13763 T18201 T18202 nummod 60,]
R13764 T18202 T18199 dobj ],described
R13765 T18203 T18178 punct .,isolated
R13766 T18204 T18205 det The,purity
R13767 T18205 T18213 nsubjpass purity,tested
R13768 T18206 T18205 prep of,purity
R13769 T18207 T18210 nummod CD4,cells
R13770 T18208 T18210 nummod +,cells
R13771 T18209 T18210 compound T,cells
R13772 T18210 T18206 pobj cells,of
R13773 T18211 T18213 auxpass was,tested
R13774 T18212 T18213 advmod initially,tested
R13775 T18213 T18213 ROOT tested,tested
R13776 T18214 T18213 agent by,tested
R13777 T18215 T18214 pobj FACS,by
R13778 T18216 T18213 cc and,tested
R13779 T18217 T18218 auxpass was,≥
R13780 T18218 T18213 conj ≥,tested
R13781 T18219 T18220 nummod 95,%
R13782 T18220 T18218 npadvmod %,≥
R13783 T18221 T18213 punct .,tested
R13784 T18222 T18228 nsubjpass Monoclonality,confirmed
R13785 T18223 T18222 prep of,Monoclonality
R13786 T18224 T18226 compound T,clones
R13787 T18225 T18226 compound cell,clones
R13788 T18226 T18223 pobj clones,of
R13789 T18227 T18228 auxpass was,confirmed
R13790 T18228 T18228 ROOT confirmed,confirmed
R13791 T18229 T18228 agent by,confirmed
R13792 T18230 T18231 amod TCR-chain,mapping
R13793 T18231 T18229 pobj mapping,by
R13794 T18232 T18228 cc and,confirmed
R13795 T18233 T18234 auxpass was,identified
R13796 T18234 T18228 conj identified,confirmed
R13797 T18235 T18236 aux to,be
R13798 T18236 T18234 xcomp be,identified
R13799 T18237 T18238 amod Vbeta8,positive
R13800 T18238 T18236 acomp positive,be
R13801 T18239 T18228 punct .,confirmed
R13802 T18240 T18241 det The,clones
R13803 T18241 T18243 nsubjpass clones,characterized
R13804 T18242 T18243 auxpass were,characterized
R13805 T18243 T18243 ROOT characterized,characterized
R13806 T18244 T18243 agent by,characterized
R13807 T18245 T18247 amod high,secretion
R13808 T18246 T18247 compound IL-4,secretion
R13809 T18247 T18244 pobj secretion,by
R13810 T18248 T18243 punct .,characterized
R13825 T18362 T18364 amod Quantitative,PCR
R13826 T18363 T18364 amod real-time,PCR
R13827 T18364 T18364 ROOT PCR,PCR
R13828 T18365 T18364 punct .,PCR
R13829 T18366 T18368 det The,primers
R13830 T18367 T18368 compound PCR,primers
R13831 T18368 T18372 nsubjpass primers,designed
R13832 T18369 T18368 cc and,primers
R13833 T18370 T18368 conj probes,primers
R13834 T18371 T18372 auxpass were,designed
R13835 T18372 T18372 ROOT designed,designed
R13836 T18373 T18372 prep based,designed
R13837 T18374 T18373 prep on,based
R13838 T18375 T18376 det the,sequences
R13839 T18376 T18374 pobj sequences,on
R13840 T18377 T18376 acl reported,sequences
R13841 T18378 T18377 prep in,reported
R13842 T18379 T18378 pobj GenBank,in
R13843 T18380 T18377 prep with,reported
R13844 T18381 T18385 det the,version
R13845 T18382 T18383 compound Primer,Express
R13846 T18383 T18385 compound Express,version
R13847 T18384 T18385 compound software,version
R13848 T18385 T18380 pobj version,with
R13849 T18386 T18385 nummod 1.2,version
R13850 T18387 T18389 punct (,Biosystems
R13851 T18388 T18389 compound Applied,Biosystems
R13852 T18389 T18385 appos Biosystems,version
R13853 T18390 T18377 punct ),reported
R13854 T18391 T18392 mark as,follows
R13855 T18392 T18372 advcl follows,designed
R13856 T18393 T18372 punct :,designed
R13857 T18394 T18396 nummod FOXP3,primer
R13858 T18395 T18396 amod forward,primer
R13859 T18396 T18396 ROOT primer,primer
R13860 T18397 T18438 meta 5,primer
R13861 T18398 T18400 punct ′,GAA
R13862 T18399 T18400 punct -,GAA
R13863 T18400 T18438 ccomp GAA,primer
R13864 T18401 T18402 compound ACA,GCA
R13865 T18402 T18426 npadvmod GCA,primer
R13866 T18403 T18426 nmod CAT,primer
R13867 T18404 T18408 nmod TCC,′
R13868 T18405 T18408 nmod CAG,′
R13869 T18406 T18408 nmod AGT,′
R13870 T18407 T18408 amod TC-3,′
R13871 T18408 T18403 appos ′,CAT
R13872 T18409 T18403 punct ;,CAT
R13873 T18410 T18412 nummod FOXP3,primer
R13874 T18411 T18412 compound reverse,primer
R13875 T18412 T18403 appos primer,CAT
R13876 T18413 T18426 nummod 5,primer
R13877 T18414 T18426 punct ′,primer
R13878 T18415 T18426 punct -,primer
R13879 T18416 T18417 compound ATG,GCC
R13880 T18417 T18426 npadvmod GCC,primer
R13881 T18418 T18422 nmod CAG,′
R13882 T18419 T18422 nmod CGG,′
R13883 T18420 T18422 nmod ATG,′
R13884 T18421 T18422 amod AG-3,′
R13885 T18422 T18417 appos ′,GCC
R13886 T18423 T18417 punct ;,GCC
R13887 T18424 T18417 conj EF-1α,GCC
R13888 T18425 T18417 advmod forward,GCC
R13889 T18426 T18400 amod primer,GAA
R13890 T18427 T18426 cc and,primer
R13891 T18428 T18426 conj reverse,primer
R13892 T18429 T18428 dobj primer,reverse
R13893 T18430 T18431 mark as,described
R13894 T18431 T18438 advcl described,primer
R13895 T18432 T18434 nmod [,]
R13896 T18433 T18434 nummod 61,]
R13897 T18434 T18431 dobj ],described
R13898 T18435 T18438 punct ;,primer
R13899 T18436 T18438 nsubj GATA3,primer
R13900 T18437 T18438 advmod forward,primer
R13901 T18438 T18438 ROOT primer,primer
R13902 T18439 T18462 nummod 5,′
R13903 T18440 T18444 punct ′,TCT
R13904 T18441 T18444 punct -,TCT
R13905 T18442 T18444 compound GCG,TCT
R13906 T18443 T18444 compound GGC,TCT
R13907 T18444 T18462 nmod TCT,′
R13908 T18445 T18446 compound ATC,ACA
R13909 T18446 T18447 compound ACA,AAA
R13910 T18447 T18462 dep AAA,′
R13911 T18448 T18449 nummod TGA-3,′
R13912 T18449 T18462 nmod ′,′
R13913 T18450 T18449 cc and,′
R13914 T18451 T18449 conj rwd,′
R13915 T18452 T18451 nummod 5,rwd
R13916 T18453 T18462 punct ′,′
R13917 T18454 T18455 punct -,GCT
R13918 T18455 T18462 compound GCT,′
R13919 T18456 T18462 compound CTC,′
R13920 T18457 T18462 compound CTG,′
R13921 T18458 T18462 nmod GCT,′
R13922 T18459 T18460 compound GCA,GAC
R13923 T18460 T18462 nmod GAC,′
R13924 T18461 T18462 amod AGC-3,′
R13925 T18462 T18438 dep ′,primer
R13926 T18463 T18438 punct .,primer
R13927 T18464 T18466 det The,cDNAs
R13928 T18465 T18466 amod prepared,cDNAs
R13929 T18466 T18468 nsubjpass cDNAs,amplified
R13930 T18467 T18468 auxpass were,amplified
R13931 T18468 T18468 ROOT amplified,amplified
R13932 T18469 T18468 advcl using,amplified
R13933 T18470 T18471 compound SYBR-PCR,mastermix
R13934 T18471 T18469 dobj mastermix,using
R13935 T18472 T18471 punct (,mastermix
R13936 T18473 T18471 appos Biorad,mastermix
R13937 T18474 T18471 punct ),mastermix
R13938 T18475 T18468 prep according,amplified
R13939 T18476 T18475 prep to,according
R13940 T18477 T18478 det the,recommendations
R13941 T18478 T18476 pobj recommendations,to
R13942 T18479 T18478 prep of,recommendations
R13943 T18480 T18481 det the,manufacturer
R13944 T18481 T18479 pobj manufacturer,of
R13945 T18482 T18478 prep in,recommendations
R13946 T18483 T18489 det an,System
R13947 T18484 T18489 compound ABI,System
R13948 T18485 T18489 compound PRISM,System
R13949 T18486 T18489 nummod 7000,System
R13950 T18487 T18489 compound Sequence,System
R13951 T18488 T18489 compound Detection,System
R13952 T18489 T18482 pobj System,in
R13953 T18490 T18489 punct (,System
R13954 T18491 T18492 compound Applied,Biosystems
R13955 T18492 T18489 appos Biosystems,System
R13956 T18493 T18489 punct ),System
R13957 T18494 T18468 punct .,amplified
R13958 T18495 T18496 compound Quantitative,PCR
R13959 T18496 T18501 nsubjpass PCR,performed
R13960 T18497 T18496 prep of,PCR
R13961 T18498 T18499 compound murine,samples
R13962 T18499 T18497 pobj samples,of
R13963 T18500 T18501 auxpass was,performed
R13964 T18501 T18501 ROOT performed,performed
R13965 T18502 T18501 prep with,performed
R13966 T18503 T18508 amod Brilliant,mix
R13967 T18504 T18506 compound SYBR,QPCR
R13968 T18505 T18506 compound Green,QPCR
R13969 T18506 T18508 compound QPCR,mix
R13970 T18507 T18508 compound master,mix
R13971 T18508 T18502 pobj mix,with
R13972 T18509 T18508 punct (,mix
R13973 T18510 T18508 appos Stratagene,mix
R13974 T18511 T18501 punct ),performed
R13975 T18512 T18501 cc and,performed
R13976 T18513 T18515 det the,primers
R13977 T18514 T18515 amod following,primers
R13978 T18515 T18501 conj primers,performed
R13979 T18516 T18515 punct :,primers
R13980 T18517 T18518 compound Ubiquitin,C
R13981 T18518 T18515 appos C,primers
R13982 T18519 T18518 punct ",",C
R13983 T18520 T18531 nummod 5,′
R13984 T18521 T18531 punct ′,′
R13985 T18522 T18531 punct -,′
R13986 T18523 T18531 compound AGG,′
R13987 T18524 T18527 compound TCA,AAG
R13988 T18525 T18527 compound AAC,AAG
R13989 T18526 T18527 compound AGG,AAG
R13990 T18527 T18531 compound AAG,′
R13991 T18528 T18531 compound ACA,′
R13992 T18529 T18531 compound GAC,′
R13993 T18530 T18531 compound GTA-3,′
R13994 T18531 T18518 appos ′,C
R13995 T18532 T18531 cc and,′
R13996 T18533 T18537 nummod 5,′
R13997 T18534 T18536 punct ′,TCACACCCAAGAACAAGCACA-3
R13998 T18535 T18536 punct -,TCACACCCAAGAACAAGCACA-3
R13999 T18536 T18537 compound TCACACCCAAGAACAAGCACA-3,′
R14000 T18537 T18531 conj ′,′
R14001 T18538 T18518 punct ;,C
R14002 T18539 T18518 conj Smad-7,C
R14003 T18540 T18539 punct ",",Smad-7
R14004 T18541 T18551 nummod 5,′
R14005 T18542 T18544 punct ′,GAA
R14006 T18543 T18544 punct -,GAA
R14007 T18544 T18551 compound GAA,′
R14008 T18545 T18549 compound ACC,AAT
R14009 T18546 T18549 compound GGG,AAT
R14010 T18547 T18549 compound GGA,AAT
R14011 T18548 T18549 compound ACG,AAT
R14012 T18549 T18551 compound AAT,′
R14013 T18550 T18551 compound TAT-3,′
R14014 T18551 T18518 appos ′,C
R14015 T18552 T18551 cc and,′
R14016 T18553 T18556 nummod 5,CGC
R14017 T18554 T18556 punct ′,CGC
R14018 T18555 T18556 punct -,CGC
R14019 T18556 T18515 appos CGC,primers
R14020 T18557 T18560 compound GAG,CCT
R14021 T18558 T18560 compound TCT,CCT
R14022 T18559 T18560 compound TCT,CCT
R14023 T18560 T18556 appos CCT,CGC
R14024 T18561 T18561 ROOT CCC,CCC
R14025 T18562 T18563 amod A-3,′
R14026 T18563 T18561 appos ′,CCC
R14027 T18564 T18561 punct ;,CCC
R14028 T18565 T18561 conj TGF-ß1,CCC
R14029 T18566 T18565 punct ",",TGF-ß1
R14030 T18567 T18578 nummod 5,′
R14031 T18568 T18570 punct ′,TGA
R14032 T18569 T18570 punct -,TGA
R14033 T18570 T18573 compound TGA,TGG
R14034 T18571 T18573 compound CGT,TGG
R14035 T18572 T18573 compound CAC,TGG
R14036 T18573 T18578 compound TGG,′
R14037 T18574 T18578 compound AGT,′
R14038 T18575 T18578 compound TGT,′
R14039 T18576 T18578 compound ACG,′
R14040 T18577 T18578 compound G-3,′
R14041 T18578 T18561 conj ′,CCC
R14042 T18579 T18578 cc and,′
R14043 T18580 T18590 nummod 5,′
R14044 T18581 T18588 punct ′,TGG
R14045 T18582 T18588 punct -,TGG
R14046 T18583 T18588 compound GGT,TGG
R14047 T18584 T18588 compound TCA,TGG
R14048 T18585 T18588 compound TGT,TGG
R14049 T18586 T18588 compound CAT,TGG
R14050 T18587 T18588 compound GGA,TGG
R14051 T18588 T18590 nmod TGG,′
R14052 T18589 T18590 nummod TGC-3,′
R14053 T18590 T18578 conj ′,′
R14054 T18591 T18590 punct .,′
R14055 T18592 T18593 compound Primer,pairs
R14056 T18593 T18595 nsubjpass pairs,evaluated
R14057 T18594 T18595 auxpass were,evaluated
R14058 T18595 T18595 ROOT evaluated,evaluated
R14059 T18596 T18595 prep for,evaluated
R14060 T18597 T18596 pobj integrity,for
R14061 T18598 T18595 agent by,evaluated
R14062 T18599 T18606 nmod analysis,curves
R14063 T18600 T18599 prep of,analysis
R14064 T18601 T18603 det the,plot
R14065 T18602 T18603 compound amplification,plot
R14066 T18603 T18600 pobj plot,of
R14067 T18604 T18603 punct ",",plot
R14068 T18605 T18606 compound dissociation,curves
R14069 T18606 T18598 pobj curves,by
R14070 T18607 T18606 punct ",",curves
R14071 T18608 T18606 cc and,curves
R14072 T18609 T18606 conj efficiency,curves
R14073 T18610 T18609 prep of,efficiency
R14074 T18611 T18612 compound PCR,amplification
R14075 T18612 T18610 pobj amplification,of
R14076 T18613 T18595 punct .,evaluated
R14077 T18614 T18615 compound PCR,conditions
R14078 T18615 T18616 nsubj conditions,were
R14079 T18616 T18616 ROOT were,were
R14080 T18617 T18618 nummod 10,min
R14081 T18618 T18616 attr min,were
R14082 T18619 T18616 prep at,were
R14083 T18620 T18621 nummod 95,°
R14084 T18621 T18622 nummod °,C
R14085 T18622 T18619 pobj C,at
R14086 T18623 T18622 punct ",",C
R14087 T18624 T18616 advcl followed,were
R14088 T18625 T18624 agent by,followed
R14089 T18626 T18627 nummod 40,cycles
R14090 T18627 T18625 pobj cycles,by
R14091 T18628 T18627 prep of,cycles
R14092 T18629 T18628 pobj 15,of
R14093 T18630 T18627 appos s,cycles
R14094 T18631 T18624 prep at,followed
R14095 T18632 T18634 nummod 95,C
R14096 T18633 T18634 nummod °,C
R14097 T18634 T18631 pobj C,at
R14098 T18635 T18624 cc and,followed
R14099 T18636 T18637 nummod 60,°
R14100 T18637 T18638 compound °,C
R14101 T18638 T18624 conj C,followed
R14102 T18639 T18638 prep for,C
R14103 T18640 T18641 nummod 1,min
R14104 T18641 T18639 pobj min,for
R14105 T18642 T18616 advcl using,were
R14106 T18643 T18647 det an,system
R14107 T18644 T18647 nummod 7300,system
R14108 T18645 T18647 amod real-time,system
R14109 T18646 T18647 compound PCR,system
R14110 T18647 T18642 dobj system,using
R14111 T18648 T18647 punct (,system
R14112 T18649 T18650 compound Applied,Biosystems
R14113 T18650 T18647 appos Biosystems,system
R14114 T18651 T18647 punct ),system
R14115 T18652 T18616 punct .,were
R14116 T18653 T18654 compound PCR,amplification
R14117 T18654 T18663 nsubjpass amplification,performed
R14118 T18655 T18654 prep of,amplification
R14119 T18656 T18658 det the,gene
R14120 T18657 T18658 compound housekeeping,gene
R14121 T18658 T18655 pobj gene,of
R14122 T18659 T18661 compound encoding,C
R14123 T18660 T18661 compound ubiquitin,C
R14124 T18661 T18663 nsubjpass C,performed
R14125 T18662 T18663 auxpass was,performed
R14126 T18663 T18663 ROOT performed,performed
R14127 T18664 T18663 prep during,performed
R14128 T18665 T18666 det each,run
R14129 T18666 T18664 pobj run,during
R14130 T18667 T18666 prep for,run
R14131 T18668 T18669 det each,sample
R14132 T18669 T18667 pobj sample,for
R14133 T18670 T18671 aux to,allow
R14134 T18671 T18663 advcl allow,performed
R14135 T18672 T18671 dobj normalization,allow
R14136 T18673 T18672 prep between,normalization
R14137 T18674 T18673 pobj samples,between
R14138 T18675 T18663 punct .,performed
R14139 T18676 T18677 compound Relative,quantification
R14140 T18677 T18686 nsubjpass quantification,performed
R14141 T18678 T18677 cc and,quantification
R14142 T18679 T18677 conj calculation,quantification
R14143 T18680 T18679 prep of,calculation
R14144 T18681 T18682 det the,range
R14145 T18682 T18680 pobj range,of
R14146 T18683 T18682 prep of,range
R14147 T18684 T18683 pobj confidence,of
R14148 T18685 T18686 auxpass was,performed
R14149 T18686 T18686 ROOT performed,performed
R14150 T18687 T18686 advcl using,performed
R14151 T18688 T18691 det the,method
R14152 T18689 T18691 amod comparative,method
R14153 T18690 T18691 compound ΔΔCT,method
R14154 T18691 T18687 dobj method,using
R14155 T18692 T18686 punct .,performed
R14254 T18896 T18899 amod Inducible,culture
R14255 T18897 T18898 compound murine,Treg
R14256 T18898 T18899 compound Treg,culture
R14257 T18899 T18899 ROOT culture,culture
R14258 T18900 T18899 punct .,culture
R14259 T18901 T18905 amod Naive,cells
R14260 T18902 T18905 nmod CD4,cells
R14261 T18903 T18905 nummod +,cells
R14262 T18904 T18905 compound T,cells
R14263 T18905 T18916 nsubj cells,were
R14264 T18906 T18905 punct (,cells
R14265 T18907 T18908 nummod CD4,+
R14266 T18908 T18905 appos +,cells
R14267 T18909 T18908 punct ",",+
R14268 T18910 T18911 compound CD62L,+
R14269 T18911 T18908 appos +,+
R14270 T18912 T18911 punct ",",+
R14271 T18913 T18911 cc and,+
R14272 T18914 T18911 conj CD25,+
R14273 T18915 T18905 punct ),cells
R14274 T18916 T18916 ROOT were,were
R14275 T18917 T18916 acomp isolated,were
R14276 T18918 T18917 prep from,isolated
R14277 T18919 T18921 amod pooled,nodes
R14278 T18920 T18921 compound lymph,nodes
R14279 T18921 T18918 pobj nodes,from
R14280 T18922 T18921 cc and,nodes
R14281 T18923 T18921 conj spleens,nodes
R14282 T18924 T18917 agent by,isolated
R14283 T18925 T18924 pobj FACS,by
R14284 T18926 T18928 punct (,Aria
R14285 T18927 T18928 compound FACS,Aria
R14286 T18928 T18916 npadvmod Aria,were
R14287 T18929 T18928 punct ",",Aria
R14288 T18930 T18931 compound BD,Biosciences
R14289 T18931 T18928 appos Biosciences,Aria
R14290 T18932 T18928 punct ),Aria
R14291 T18933 T18916 punct .,were
R14292 T18934 T18936 quantmod 5,105
R14293 T18935 T18936 quantmod ×,105
R14294 T18936 T18938 nummod 105,cells
R14295 T18937 T18938 compound T,cells
R14296 T18938 T18940 nsubjpass cells,co-cultured
R14297 T18939 T18940 auxpass were,co-cultured
R14298 T18940 T18940 ROOT co-cultured,co-cultured
R14299 T18941 T18940 prep with,co-cultured
R14300 T18942 T18944 quantmod 2.5,104
R14301 T18943 T18944 quantmod ×,104
R14302 T18944 T18946 nummod 104,marrow
R14303 T18945 T18946 compound bone,marrow
R14304 T18946 T18941 pobj marrow,with
R14305 T18947 T18949 amod derived,cells
R14306 T18948 T18949 amod dendritic,cells
R14307 T18949 T18946 appos cells,marrow
R14308 T18950 T18952 nmod [,]
R14309 T18951 T18952 nummod 62,]
R14310 T18952 T18949 appos ],cells
R14311 T18953 T18952 cc and,]
R14312 T18954 T18958 compound 0.01,OVA323
R14313 T18955 T18958 compound μg,OVA323
R14314 T18956 T18958 compound /,OVA323
R14315 T18957 T18958 compound ml,OVA323
R14316 T18958 T18952 conj OVA323,]
R14317 T18959 T18960 nummod 339,peptide
R14318 T18960 T18949 appos peptide,cells
R14319 T18961 T18962 punct (,Ansynth
R14320 T18962 T18960 appos Ansynth,peptide
R14321 T18963 T18949 punct ),cells
R14322 T18964 T18946 prep in,marrow
R14323 T18965 T18966 det the,presence
R14324 T18966 T18964 pobj presence,in
R14325 T18967 T18966 cc or,presence
R14326 T18968 T18966 conj absence,presence
R14327 T18969 T18968 prep of,absence
R14328 T18970 T18973 amod 20-ng,rhTGF-ß1
R14329 T18971 T18973 compound /,rhTGF-ß1
R14330 T18972 T18973 compound μl,rhTGF-ß1
R14331 T18973 T18969 pobj rhTGF-ß1,of
R14332 T18974 T18975 punct (,Peprotech
R14333 T18975 T18973 appos Peprotech,rhTGF-ß1
R14334 T18976 T18975 punct ),Peprotech
R14335 T18977 T18966 prep in,presence
R14336 T18978 T18979 amod 48-well,plates
R14337 T18979 T18977 pobj plates,in
R14338 T18980 T18940 punct .,co-cultured
R14339 T18981 T18987 mark After,harvested
R14340 T18982 T18987 meta 4,harvested
R14341 T18983 T18982 advmod d,4
R14342 T18984 T18987 punct ",",harvested
R14343 T18985 T18987 nsubjpass cells,harvested
R14344 T18986 T18987 auxpass were,harvested
R14345 T18987 T18987 ROOT harvested,harvested
R14346 T18988 T18987 cc and,harvested
R14347 T18989 T18987 conj analyzed,harvested
R14348 T18990 T18989 prep for,analyzed
R14349 T18991 T18993 amod intracellular,expression
R14350 T18992 T18993 nummod FOXP3,expression
R14351 T18993 T18990 pobj expression,for
R14352 T18994 T18993 prep by,expression
R14353 T18995 T18994 pobj FACS,by
R14354 T18996 T18995 cc or,FACS
R14355 T18997 T18998 compound gene,expression
R14356 T18998 T18993 conj expression,expression
R14357 T18999 T18993 prep by,expression
R14358 T19000 T19001 amod quantitative,RT-PCR
R14359 T19001 T18999 pobj RT-PCR,by
R14360 T19002 T18987 punct .,harvested
R14484 T19255 T19255 ROOT In,In
R14485 T19256 T19259 amod vitro,differentiation
R14486 T19257 T19259 compound T,differentiation
R14487 T19258 T19259 compound cell,differentiation
R14488 T19259 T19255 pobj differentiation,In
R14489 T19260 T19255 punct .,In
R14490 T19261 T19265 nummod CD4,magnetically-sorted
R14491 T19262 T19265 nummod +,magnetically-sorted
R14492 T19263 T19265 compound CD45RA,magnetically-sorted
R14493 T19264 T19265 compound +,magnetically-sorted
R14494 T19265 T19272 meta magnetically-sorted,according
R14495 T19266 T19265 punct (,magnetically-sorted
R14496 T19267 T19268 nummod CD45RO,depletion
R14497 T19268 T19265 appos depletion,magnetically-sorted
R14498 T19269 T19268 punct ",",depletion
R14499 T19270 T19268 appos MACS,depletion
R14500 T19271 T19265 punct ",",magnetically-sorted
R14501 T19272 T19282 prep according,stimulated
R14502 T19273 T19272 prep to,according
R14503 T19274 T19275 det the,protocol
R14504 T19275 T19273 pobj protocol,to
R14505 T19276 T19275 prep of,protocol
R14506 T19277 T19278 det the,manufacturer
R14507 T19278 T19276 pobj manufacturer,of
R14508 T19279 T19282 punct ),stimulated
R14509 T19280 T19282 nsubjpass cells,stimulated
R14510 T19281 T19282 auxpass were,stimulated
R14511 T19282 T19282 ROOT stimulated,stimulated
R14512 T19283 T19282 prep with,stimulated
R14513 T19284 T19286 amod immobilized,anti-CD3
R14514 T19285 T19286 amod plate-bound,anti-CD3
R14515 T19286 T19283 pobj anti-CD3,with
R14516 T19287 T19291 punct (,ml
R14517 T19288 T19289 nummod 1,μg
R14518 T19289 T19291 compound μg,ml
R14519 T19290 T19291 compound /,ml
R14520 T19291 T19286 appos ml,anti-CD3
R14521 T19292 T19291 punct ",",ml
R14522 T19293 T19291 conj Okt3,ml
R14523 T19294 T19293 punct ",",Okt3
R14524 T19295 T19293 appos IgG1,Okt3
R14525 T19296 T19293 punct ),Okt3
R14526 T19297 T19293 cc and,Okt3
R14527 T19298 T19293 conj anti-CD28,Okt3
R14528 T19299 T19303 punct (,ml
R14529 T19300 T19301 nummod 2,μg
R14530 T19301 T19303 nmod μg,ml
R14531 T19302 T19303 compound /,ml
R14532 T19303 T19298 appos ml,anti-CD28
R14533 T19304 T19303 punct ),ml
R14534 T19305 T19298 prep in,anti-CD28
R14535 T19306 T19307 amod Th1,conditions
R14536 T19307 T19305 pobj conditions,in
R14537 T19308 T19291 punct :,ml
R14538 T19309 T19311 nummod 25,IL-12
R14539 T19310 T19311 compound ng/ml,IL-12
R14540 T19311 T19291 appos IL-12,ml
R14541 T19312 T19311 punct ",",IL-12
R14542 T19313 T19317 nummod 5,anti-IL-4
R14543 T19314 T19317 nmod μg,anti-IL-4
R14544 T19315 T19317 nmod /,anti-IL-4
R14545 T19316 T19317 compound ml,anti-IL-4
R14546 T19317 T19311 appos anti-IL-4,IL-12
R14547 T19318 T19320 punct (,systems
R14548 T19319 T19320 compound R&D,systems
R14549 T19320 T19317 appos systems,anti-IL-4
R14550 T19321 T19320 punct ),systems
R14551 T19322 T19282 punct ;,stimulated
R14552 T19323 T19323 ROOT in,in
R14553 T19324 T19325 amod Th2,conditions
R14554 T19325 T19323 pobj conditions,in
R14555 T19326 T19323 punct :,in
R14556 T19327 T19329 nummod 25,IL-4
R14557 T19328 T19329 compound ng/ml,IL-4
R14558 T19329 T19329 ROOT IL-4,IL-4
R14559 T19330 T19329 punct ",",IL-4
R14560 T19331 T19332 nummod 5,μg
R14561 T19332 T19329 appos μg,IL-4
R14562 T19333 T19335 nmod /,anti-IFN-γ
R14563 T19334 T19335 compound ml,anti-IFN-γ
R14564 T19335 T19332 appos anti-IFN-γ,μg
R14565 T19336 T19335 punct ",",anti-IFN-γ
R14566 T19337 T19338 nummod 5,μg
R14567 T19338 T19341 nmod μg,anti-IL-12
R14568 T19339 T19341 nmod /,anti-IL-12
R14569 T19340 T19341 compound ml,anti-IL-12
R14570 T19341 T19335 appos anti-IL-12,anti-IFN-γ
R14571 T19342 T19344 punct (,systems
R14572 T19343 T19344 compound R&D,systems
R14573 T19344 T19341 appos systems,anti-IL-12
R14574 T19345 T19344 punct ),systems
R14575 T19346 T19344 punct ;,systems
R14576 T19347 T19335 cc or,anti-IFN-γ
R14577 T19348 T19335 prep in,anti-IFN-γ
R14578 T19349 T19350 compound Treg,conditions
R14579 T19350 T19348 pobj conditions,in
R14580 T19351 T19332 punct :,μg
R14581 T19352 T19353 nummod 10,ng/ml
R14582 T19353 T19354 compound ng/ml,TGF-β
R14583 T19354 T19332 appos TGF-β,μg
R14584 T19355 T19354 punct ",",TGF-β
R14585 T19356 T19357 nummod 5,μg
R14586 T19357 T19360 compound μg,anti-IFN-γ
R14587 T19358 T19360 compound /,anti-IFN-γ
R14588 T19359 T19360 compound ml,anti-IFN-γ
R14589 T19360 T19354 appos anti-IFN-γ,TGF-β
R14590 T19361 T19360 punct ",",anti-IFN-γ
R14591 T19362 T19363 nummod 5,μg
R14592 T19363 T19366 nmod μg,anti-IL-12
R14593 T19364 T19366 nmod /,anti-IL-12
R14594 T19365 T19366 compound ml,anti-IL-12
R14595 T19366 T19360 appos anti-IL-12,anti-IFN-γ
R14596 T19367 T19366 punct ",",anti-IL-12
R14597 T19368 T19369 nummod 5,μg
R14598 T19369 T19372 nmod μg,anti-IL-4
R14599 T19370 T19372 nmod /,anti-IL-4
R14600 T19371 T19372 compound ml,anti-IL-4
R14601 T19372 T19366 appos anti-IL-4,anti-IL-12
R14602 T19373 T19282 punct .,stimulated
R14603 T19374 T19375 amod Proliferating,cells
R14604 T19375 T19377 nsubjpass cells,expanded
R14605 T19376 T19377 auxpass were,expanded
R14606 T19377 T19377 ROOT expanded,expanded
R14607 T19378 T19377 prep in,expanded
R14608 T19379 T19380 npadvmod medium,containing
R14609 T19380 T19378 pobj containing,in
R14610 T19381 T19380 dobj IL-2,containing
R14611 T19382 T19381 punct (,IL-2
R14612 T19383 T19384 nummod 30,ng/ml
R14613 T19384 T19381 appos ng/ml,IL-2
R14614 T19385 T19381 punct ),IL-2
R14615 T19386 T19377 punct .,expanded
R14706 T19618 T19618 ROOT Cloning,Cloning
R14707 T19619 T19618 prep of,Cloning
R14708 T19620 T19622 det the,promoter
R14709 T19621 T19622 nummod FOXP3,promoter
R14710 T19622 T19619 pobj promoter,of
R14711 T19623 T19622 cc and,promoter
R14712 T19624 T19622 conj construction,promoter
R14713 T19625 T19622 prep of,promoter
R14714 T19626 T19627 amod mutant,constructs
R14715 T19627 T19625 pobj constructs,of
R14716 T19628 T19618 punct .,Cloning
R14717 T19629 T19631 det The,promoter
R14718 T19630 T19631 nummod FOXP3,promoter
R14719 T19631 T19633 nsubjpass promoter,cloned
R14720 T19632 T19633 auxpass was,cloned
R14721 T19633 T19633 ROOT cloned,cloned
R14722 T19634 T19633 prep into,cloned
R14723 T19635 T19638 det the,vector
R14724 T19636 T19638 amod pGL3,vector
R14725 T19637 T19638 amod basic,vector
R14726 T19638 T19634 pobj vector,into
R14727 T19639 T19638 punct (,vector
R14728 T19640 T19641 compound Promega,Biotech
R14729 T19641 T19638 appos Biotech,vector
R14730 T19642 T19638 punct ),vector
R14731 T19643 T19644 aux to,generate
R14732 T19644 T19633 advcl generate,cloned
R14733 T19645 T19654 det the,]
R14734 T19646 T19654 amod pGL3,]
R14735 T19647 T19654 nummod FOXP3,]
R14736 T19648 T19654 nummod −,]
R14737 T19649 T19650 nummod 511,/
R14738 T19650 T19654 nsubj /,]
R14739 T19651 T19654 nummod +176,]
R14740 T19652 T19654 nmod [,]
R14741 T19653 T19654 nummod 24,]
R14742 T19654 T19644 dobj ],generate
R14743 T19655 T19633 punct .,cloned
R14744 T19656 T19657 amod Site-directed,mutagenesis
R14745 T19657 T19664 nsubjpass mutagenesis,introduced
R14746 T19658 T19657 prep in,mutagenesis
R14747 T19659 T19662 det the,region
R14748 T19660 T19662 nummod FOXP3,region
R14749 T19661 T19662 compound promoter,region
R14750 T19662 T19658 pobj region,in
R14751 T19663 T19664 auxpass were,introduced
R14752 T19664 T19664 ROOT introduced,introduced
R14753 T19665 T19664 advcl using,introduced
R14754 T19666 T19668 det the,kit
R14755 T19667 T19668 compound QuickChange,kit
R14756 T19668 T19665 dobj kit,using
R14757 T19669 T19668 punct (,kit
R14758 T19670 T19668 appos Stratagene,kit
R14759 T19671 T19668 punct ),kit
R14760 T19672 T19664 punct ",",introduced
R14761 T19673 T19664 prep according,introduced
R14762 T19674 T19673 prep to,according
R14763 T19675 T19676 det the,manufacturer
R14764 T19676 T19678 poss manufacturer,instructions
R14765 T19677 T19676 case 's,manufacturer
R14766 T19678 T19674 pobj instructions,to
R14767 T19679 T19664 punct .,introduced
R14768 T19680 T19682 det The,primer
R14769 T19681 T19682 amod following,primer
R14770 T19682 T19688 nsubjpass primer,used
R14771 T19683 T19682 cc and,primer
R14772 T19684 T19686 poss its,strand
R14773 T19685 T19686 amod complementary,strand
R14774 T19686 T19682 conj strand,primer
R14775 T19687 T19688 auxpass were,used
R14776 T19688 T19688 ROOT used,used
R14777 T19689 T19706 punct :,GA.
R14778 T19690 T19691 compound GTT,TCT
R14779 T19691 T19706 compound TCT,GA.
R14780 T19692 T19706 compound CAT,GA.
R14781 T19693 T19699 compound GAG,CTT
R14782 T19694 T19699 compound CCC,CTT
R14783 T19695 T19699 compound TAT,CTT
R14784 T19696 T19699 compound TAA,CTT
R14785 T19697 T19699 compound GTC,CTT
R14786 T19698 T19699 compound ATT,CTT
R14787 T19699 T19706 compound CTT,GA.
R14788 T19700 T19706 compound ACC,GA.
R14789 T19701 T19706 compound TCT,GA.
R14790 T19702 T19706 compound CAC,GA.
R14791 T19703 T19706 compound CTC,GA.
R14792 T19704 T19706 compound TGT,GA.
R14793 T19705 T19706 compound GGT,GA.
R14794 T19706 T19688 npadvmod GA.,used
R14901 T19885 T19885 ROOT Transfections,Transfections
R14902 T19886 T19885 cc and,Transfections
R14903 T19887 T19889 compound reporter,assays
R14904 T19888 T19889 compound gene,assays
R14905 T19889 T19885 conj assays,Transfections
R14906 T19890 T19885 punct .,Transfections
R14907 T19891 T19892 compound T,cells
R14908 T19892 T19894 nsubjpass cells,rested
R14909 T19893 T19894 auxpass were,rested
R14910 T19894 T19894 ROOT rested,rested
R14911 T19895 T19894 prep in,rested
R14912 T19896 T19898 amod serum-free,medium
R14913 T19897 T19898 compound AIM-V,medium
R14914 T19898 T19895 pobj medium,in
R14915 T19899 T19901 punct (,Technologies
R14916 T19900 T19901 compound Life,Technologies
R14917 T19901 T19898 appos Technologies,medium
R14918 T19902 T19898 punct ),medium
R14919 T19903 T19894 advmod overnight,rested
R14920 T19904 T19894 punct .,rested
R14921 T19905 T19906 nummod 3.5,μg
R14922 T19906 T19919 nsubjpass μg,added
R14923 T19907 T19906 prep of,μg
R14924 T19908 T19913 det the,vector
R14925 T19909 T19913 nummod FOXP3,vector
R14926 T19910 T19913 nmod promoter,vector
R14927 T19911 T19913 nmod luciferase,vector
R14928 T19912 T19913 compound reporter,vector
R14929 T19913 T19907 pobj vector,of
R14930 T19914 T19906 cc and,μg
R14931 T19915 T19916 nummod 0.5,μg
R14932 T19916 T19917 compound μg,phRL-TK
R14933 T19917 T19906 conj phRL-TK,μg
R14934 T19918 T19919 auxpass were,added
R14935 T19919 T19919 ROOT added,added
R14936 T19920 T19919 prep to,added
R14937 T19921 T19923 quantmod 3,106
R14938 T19922 T19923 quantmod ×,106
R14939 T19923 T19927 nummod 106,cells
R14940 T19924 T19927 nmod CD4,cells
R14941 T19925 T19927 nummod +,cells
R14942 T19926 T19927 compound T,cells
R14943 T19927 T19920 pobj cells,to
R14944 T19928 T19919 oprd resuspended,added
R14945 T19929 T19928 prep in,resuspended
R14946 T19930 T19931 nummod 100,μL
R14947 T19931 T19929 pobj μL,in
R14948 T19932 T19931 prep of,μL
R14949 T19933 T19934 compound Nucleofector,solution
R14950 T19934 T19932 pobj solution,of
R14951 T19935 T19937 punct (,Biosystems
R14952 T19936 T19937 compound Amaxa,Biosystems
R14953 T19937 T19934 appos Biosystems,solution
R14954 T19938 T19937 punct ),Biosystems
R14955 T19939 T19928 cc and,resuspended
R14956 T19940 T19928 conj electroporated,resuspended
R14957 T19941 T19940 xcomp using,electroporated
R14958 T19942 T19944 det the,program
R14959 T19943 T19944 compound U-15,program
R14960 T19944 T19941 dobj program,using
R14961 T19945 T19944 prep of,program
R14962 T19946 T19947 det the,Nucleofector
R14963 T19947 T19945 pobj Nucleofector,of
R14964 T19948 T19919 punct .,added
R14965 T19949 T19967 prep After,measured
R14966 T19950 T19952 det a,culture
R14967 T19951 T19952 amod 24-h,culture
R14968 T19952 T19949 pobj culture,After
R14969 T19953 T19952 prep in,culture
R14970 T19954 T19955 amod serum-free,conditions
R14971 T19955 T19953 pobj conditions,in
R14972 T19956 T19955 cc and,conditions
R14973 T19957 T19955 conj stimuli,conditions
R14974 T19958 T19959 mark as,indicated
R14975 T19959 T19967 advcl indicated,measured
R14976 T19960 T19959 prep in,indicated
R14977 T19961 T19962 det the,figures
R14978 T19962 T19960 pobj figures,in
R14979 T19963 T19967 punct ",",measured
R14980 T19964 T19965 compound luciferase,activity
R14981 T19965 T19967 nsubjpass activity,measured
R14982 T19966 T19967 auxpass was,measured
R14983 T19967 T19967 ROOT measured,measured
R14984 T19968 T19967 agent by,measured
R14985 T19969 T19973 det the,system
R14986 T19970 T19973 amod dual,system
R14987 T19971 T19973 compound luciferase,system
R14988 T19972 T19973 compound assay,system
R14989 T19973 T19968 pobj system,by
R14990 T19974 T19973 punct (,system
R14991 T19975 T19976 compound Promega,Biotech
R14992 T19976 T19973 appos Biotech,system
R14993 T19977 T19973 punct ),system
R14994 T19978 T19967 prep according,measured
R14995 T19979 T19978 prep to,according
R14996 T19980 T19981 det the,manufacturer
R14997 T19981 T19983 poss manufacturer,instructions
R14998 T19982 T19981 case 's,manufacturer
R14999 T19983 T19979 pobj instructions,to
R15000 T19984 T19967 punct .,measured
R15001 T19985 T19987 nsubjpass Data,normalized
R15002 T19986 T19987 auxpass were,normalized
R15003 T19987 T19987 ROOT normalized,normalized
R15004 T19988 T19987 agent by,normalized
R15005 T19989 T19990 det the,activity
R15006 T19990 T19988 pobj activity,by
R15007 T19991 T19990 prep of,activity
R15008 T19992 T19993 compound renilla,luciferase
R15009 T19993 T19991 pobj luciferase,of
R15010 T19994 T19987 punct .,normalized
R15056 T20100 T20101 compound RNA,isolation
R15057 T20101 T20101 ROOT isolation,isolation
R15058 T20102 T20101 cc and,isolation
R15059 T20103 T20104 compound cDNA,synthesis
R15060 T20104 T20101 conj synthesis,isolation
R15061 T20105 T20101 punct .,isolation
R15062 T20106 T20108 nsubjpass RNA,isolated
R15063 T20107 T20108 auxpass was,isolated
R15064 T20108 T20108 ROOT isolated,isolated
R15065 T20109 T20108 advcl using,isolated
R15066 T20110 T20113 det the,Kit
R15067 T20111 T20113 compound RNeasy,Kit
R15068 T20112 T20113 compound Mini,Kit
R15069 T20113 T20109 dobj Kit,using
R15070 T20114 T20115 punct (,Qiagen
R15071 T20115 T20113 appos Qiagen,Kit
R15072 T20116 T20113 punct ),Kit
R15073 T20117 T20108 prep according,isolated
R15074 T20118 T20117 prep to,according
R15075 T20119 T20120 det the,manufacturer
R15076 T20120 T20122 poss manufacturer,protocol
R15077 T20121 T20120 case 's,manufacturer
R15078 T20122 T20118 pobj protocol,to
R15079 T20123 T20108 punct .,isolated
R15080 T20124 T20124 ROOT Reverse,Reverse
R15081 T20125 T20130 nsubjpass transcription,performed
R15082 T20126 T20125 prep of,transcription
R15083 T20127 T20128 amod human,samples
R15084 T20128 T20126 pobj samples,of
R15085 T20129 T20130 auxpass was,performed
R15086 T20130 T20124 ccomp performed,Reverse
R15087 T20131 T20130 prep with,performed
R15088 T20132 T20134 nmod TaqMan,reagents
R15089 T20133 T20134 compound reverse-transcription,reagents
R15090 T20134 T20131 pobj reagents,with
R15091 T20135 T20137 punct (,Biosystems
R15092 T20136 T20137 compound Applied,Biosystems
R15093 T20137 T20134 appos Biosystems,reagents
R15094 T20138 T20130 punct ),performed
R15095 T20139 T20130 prep with,performed
R15096 T20140 T20141 amod random,hexamers
R15097 T20141 T20139 pobj hexamers,with
R15098 T20142 T20124 prep according,Reverse
R15099 T20143 T20142 prep to,according
R15100 T20144 T20145 det the,manufacturer
R15101 T20145 T20147 poss manufacturer,protocol
R15102 T20146 T20145 case 's,manufacturer
R15103 T20147 T20143 pobj protocol,to
R15104 T20148 T20124 punct .,Reverse
R15189 T20272 T20274 amod Recombinant,proteins
R15190 T20273 T20274 compound TAT,proteins
R15191 T20274 T20274 ROOT proteins,proteins
R15192 T20275 T20274 punct .,proteins
R15193 T20276 T20280 det The,protein
R15194 T20277 T20278 nsubj cDNAs,encoding
R15195 T20278 T20280 amod encoding,protein
R15196 T20279 T20280 nummod GATA3,protein
R15197 T20280 T20293 nsubjpass protein,cloned
R15198 T20281 T20280 cc or,protein
R15199 T20282 T20284 det the,GATA3
R15200 T20283 T20284 amod truncated,GATA3
R15201 T20284 T20280 conj GATA3,protein
R15202 T20285 T20286 punct (,lacking
R15203 T20286 T20284 acl lacking,GATA3
R15204 T20287 T20290 det the,fingers
R15205 T20288 T20290 nummod two,fingers
R15206 T20289 T20290 compound zinc,fingers
R15207 T20290 T20286 dobj fingers,lacking
R15208 T20291 T20290 punct ),fingers
R15209 T20292 T20293 auxpass were,cloned
R15210 T20293 T20293 ROOT cloned,cloned
R15211 T20294 T20293 prep in,cloned
R15212 T20295 T20294 pobj frame,in
R15213 T20296 T20293 prep into,cloned
R15214 T20297 T20299 det an,vector
R15215 T20298 T20299 compound expression,vector
R15216 T20299 T20296 pobj vector,into
R15217 T20300 T20299 prep along,vector
R15218 T20301 T20300 prep with,along
R15219 T20302 T20304 det the,sequence
R15220 T20303 T20304 compound TAT,sequence
R15221 T20304 T20301 pobj sequence,with
R15222 T20305 T20307 mark as,described
R15223 T20306 T20307 advmod previously,described
R15224 T20307 T20293 advcl described,cloned
R15225 T20308 T20310 nmod [,]
R15226 T20309 T20310 nummod 63,]
R15227 T20310 T20307 dobj ],described
R15228 T20311 T20293 punct .,cloned
R15229 T20312 T20314 nsubjpass Proteins,expressed
R15230 T20313 T20314 auxpass were,expressed
R15231 T20314 T20314 ROOT expressed,expressed
R15232 T20315 T20314 prep in,expressed
R15233 T20316 T20317 compound BL21,Star
R15234 T20317 T20315 pobj Star,in
R15235 T20318 T20317 punct (,Star
R15236 T20319 T20317 appos DE3,Star
R15237 T20320 T20317 punct ),Star
R15238 T20321 T20328 nsubjpass pLysS,purified
R15239 T20322 T20323 punct (,Invitrogen
R15240 T20323 T20321 appos Invitrogen,pLysS
R15241 T20324 T20323 punct ),Invitrogen
R15242 T20325 T20321 cc and,pLysS
R15243 T20326 T20321 conj lysates,pLysS
R15244 T20327 T20328 auxpass were,purified
R15245 T20328 T20314 advcl purified,expressed
R15246 T20329 T20328 agent by,purified
R15247 T20330 T20329 pobj Ni2,by
R15248 T20331 T20333 punct +,chelate
R15249 T20332 T20333 punct -,chelate
R15250 T20333 T20334 compound chelate,column
R15251 T20334 T20335 compound column,chromatography
R15252 T20335 T20328 prep chromatography,purified
R15253 T20336 T20314 punct .,expressed
R15254 T20337 T20339 det Both,proteins
R15255 T20338 T20339 amod TAT-linked,proteins
R15256 T20339 T20340 nsubj proteins,were
R15257 T20340 T20340 ROOT were,were
R15258 T20341 T20343 amod more,95
R15259 T20342 T20343 quantmod than,95
R15260 T20343 T20344 nummod 95,%
R15261 T20344 T20345 npadvmod %,pure
R15262 T20345 T20340 acomp pure,were
R15263 T20346 T20340 punct ",",were
R15264 T20347 T20340 prep based,were
R15265 T20348 T20347 prep on,based
R15266 T20349 T20351 nmod Coomassie,staining
R15267 T20350 T20351 amod blue,staining
R15268 T20351 T20348 pobj staining,on
R15269 T20352 T20351 prep of,staining
R15270 T20353 T20356 compound sodium,gels
R15271 T20354 T20356 compound disulfate,gels
R15272 T20355 T20356 compound acrylamide,gels
R15273 T20356 T20352 pobj gels,of
R15274 T20357 T20340 punct .,were
R15368 T20544 T20545 nummod TAT-GATA3,transduction
R15369 T20545 T20545 ROOT transduction,transduction
R15370 T20546 T20545 punct .,transduction
R15371 T20547 T20551 nummod CD4,cells
R15372 T20548 T20551 nummod +,cells
R15373 T20549 T20551 compound CD45RA,cells
R15374 T20550 T20551 compound +,cells
R15375 T20551 T20552 nsubj cells,were
R15376 T20552 T20552 ROOT were,were
R15377 T20553 T20552 acomp cultured,were
R15378 T20554 T20553 prep in,cultured
R15379 T20555 T20556 compound AIMV,medium
R15380 T20556 T20554 pobj medium,in
R15381 T20557 T20556 cc and,medium
R15382 T20558 T20552 prep transduced,were
R15383 T20559 T20558 prep with,transduced
R15384 T20560 T20561 nummod 20,nM
R15385 T20561 T20559 pobj nM,with
R15386 T20562 T20561 punct ",",nM
R15387 T20563 T20564 nummod 10,nM
R15388 T20564 T20561 conj nM,nM
R15389 T20565 T20564 punct ",",nM
R15390 T20566 T20564 cc or,nM
R15391 T20567 T20568 nummod 500,nM
R15392 T20568 T20564 conj nM,nM
R15393 T20569 T20568 prep of,nM
R15394 T20570 T20573 amod full-length,GATA3
R15395 T20571 T20570 cc or,full-length
R15396 T20572 T20570 conj truncated,full-length
R15397 T20573 T20569 pobj GATA3,of
R15398 T20574 T20568 prep over,nM
R15399 T20575 T20576 det the,course
R15400 T20576 T20574 pobj course,over
R15401 T20577 T20576 prep of,course
R15402 T20578 T20579 nummod 4,h
R15403 T20579 T20577 pobj h,of
R15404 T20580 T20552 punct .,were
R15405 T20581 T20588 prep After,washed
R15406 T20582 T20583 nummod 4,h
R15407 T20583 T20581 pobj h,After
R15408 T20584 T20588 punct ",",washed
R15409 T20585 T20586 det the,cells
R15410 T20586 T20588 nsubjpass cells,washed
R15411 T20587 T20588 auxpass were,washed
R15412 T20588 T20588 ROOT washed,washed
R15413 T20589 T20588 cc and,washed
R15414 T20590 T20588 conj activated,washed
R15415 T20591 T20590 prep with,activated
R15416 T20592 T20593 amod soluble,anti-CD3
R15417 T20593 T20591 pobj anti-CD3,with
R15418 T20594 T20593 cc and,anti-CD3
R15419 T20595 T20593 conj anti-CD28,anti-CD3
R15420 T20596 T20595 cc and,anti-CD28
R15421 T20597 T20595 conj TGF-β,anti-CD28
R15422 T20598 T20600 punct (,ng/ml
R15423 T20599 T20600 nummod 10,ng/ml
R15424 T20600 T20597 appos ng/ml,TGF-β
R15425 T20601 T20600 punct ),ng/ml
R15426 T20602 T20588 punct .,washed
R15427 T20603 T20604 det Each,day
R15428 T20604 T20611 npadvmod day,added
R15429 T20605 T20611 punct ",",added
R15430 T20606 T20608 det the,proteins
R15431 T20607 T20608 compound TAT,proteins
R15432 T20608 T20611 nsubjpass proteins,added
R15433 T20609 T20611 auxpass were,added
R15434 T20610 T20611 advmod freshly,added
R15435 T20611 T20611 ROOT added,added
R15436 T20612 T20611 prep to,added
R15437 T20613 T20614 det the,medium
R15438 T20614 T20612 pobj medium,to
R15439 T20615 T20611 punct .,added
R15440 T20616 T20617 nummod FOXP3,expression
R15441 T20617 T20619 nsubjpass expression,measured
R15442 T20618 T20619 auxpass was,measured
R15443 T20619 T20619 ROOT measured,measured
R15444 T20620 T20619 prep after,measured
R15445 T20621 T20622 nummod 5,d
R15446 T20622 T20620 pobj d,after
R15447 T20623 T20622 prep by,d
R15448 T20624 T20625 amod intracellular,staining
R15449 T20625 T20623 pobj staining,by
R15450 T20626 T20619 punct .,measured
R15530 T20794 T20796 amod Intracellular,staining
R15531 T20795 T20796 compound cytokine,staining
R15532 T20796 T20796 ROOT staining,staining
R15533 T20797 T20796 punct .,staining
R15534 T20798 T20799 compound T,cells
R15535 T20799 T20801 nsubjpass cells,stimulated
R15536 T20800 T20801 auxpass were,stimulated
R15537 T20801 T20801 ROOT stimulated,stimulated
R15538 T20802 T20801 prep with,stimulated
R15539 T20803 T20807 quantmod 2,7
R15540 T20804 T20807 quantmod ×,7
R15541 T20805 T20807 quantmod 10,7
R15542 T20806 T20807 quantmod −,7
R15543 T20807 T20809 nummod 7,PMA
R15544 T20808 T20809 compound M,PMA
R15545 T20809 T20802 pobj PMA,with
R15546 T20810 T20809 cc and,PMA
R15547 T20811 T20812 nummod 1,μg
R15548 T20812 T20814 compound μg,ml
R15549 T20813 T20814 compound /,ml
R15550 T20814 T20809 conj ml,PMA
R15551 T20815 T20814 prep of,ml
R15552 T20816 T20815 pobj ionomycin,of
R15553 T20817 T20819 punct (,Chemicals
R15554 T20818 T20819 compound Sigma,Chemicals
R15555 T20819 T20816 parataxis Chemicals,ionomycin
R15556 T20820 T20814 punct ),ml
R15557 T20821 T20801 prep for,stimulated
R15558 T20822 T20823 nummod 4,h
R15559 T20823 T20821 pobj h,for
R15560 T20824 T20801 punct .,stimulated
R15561 T20825 T20827 det The,mAb
R15562 T20826 T20827 amod following,mAb
R15563 T20827 T20829 nsubjpass mAb,used
R15564 T20828 T20829 auxpass was,used
R15565 T20829 T20829 ROOT used,used
R15566 T20830 T20829 punct :,used
R15567 T20831 T20838 meta anti-IL-4-PE,.
R15568 T20832 T20831 punct (,anti-IL-4-PE
R15569 T20833 T20834 nummod 8D4,8
R15570 T20834 T20831 appos 8,anti-IL-4-PE
R15571 T20835 T20831 punct ",",anti-IL-4-PE
R15572 T20836 T20831 appos BD,anti-IL-4-PE
R15573 T20837 T20831 punct ),anti-IL-4-PE
R15574 T20838 T20829 punct .,used
R15575 T20839 T20841 amod Matched,controls
R15576 T20840 T20841 compound isotype,controls
R15577 T20841 T20843 nsubjpass controls,used
R15578 T20842 T20843 auxpass were,used
R15579 T20843 T20843 ROOT used,used
R15580 T20844 T20843 prep at,used
R15581 T20845 T20848 det the,concentration
R15582 T20846 T20848 amod same,concentration
R15583 T20847 T20848 compound protein,concentration
R15584 T20848 T20844 pobj concentration,at
R15585 T20849 T20848 prep as,concentration
R15586 T20850 T20852 det the,antibodies
R15587 T20851 T20852 amod respective,antibodies
R15588 T20852 T20849 pobj antibodies,as
R15589 T20853 T20843 punct .,used
R15590 T20854 T20855 compound Four-color,FACS
R15591 T20855 T20857 nsubjpass FACS,performed
R15592 T20856 T20857 auxpass was,performed
R15593 T20857 T20857 ROOT performed,performed
R15594 T20858 T20857 advcl using,performed
R15595 T20859 T20861 det an,XL-MCL
R15596 T20860 T20861 compound EPICS,XL-MCL
R15597 T20861 T20858 dobj XL-MCL,using
R15598 T20862 T20864 punct (,Coulter
R15599 T20863 T20864 compound Beckman,Coulter
R15600 T20864 T20861 appos Coulter,XL-MCL
R15601 T20865 T20861 punct ),XL-MCL
R15602 T20866 T20857 advcl using,performed
R15603 T20867 T20870 det the,version
R15604 T20868 T20870 compound software,version
R15605 T20869 T20870 compound Expo32,version
R15606 T20870 T20866 dobj version,using
R15607 T20871 T20870 prep for,version
R15608 T20872 T20873 compound data,acquisition
R15609 T20873 T20871 pobj acquisition,for
R15610 T20874 T20873 cc and,acquisition
R15611 T20875 T20873 conj evaluation,acquisition
R15612 T20876 T20857 punct .,performed
R15901 T21273 T21274 compound Flow,cytometry
R15902 T21274 T21274 ROOT cytometry,cytometry
R15903 T21275 T21274 punct .,cytometry
R15904 T21276 T21289 prep For,stained
R15905 T21277 T21276 pobj analysis,For
R15906 T21278 T21277 prep of,analysis
R15907 T21279 T21280 nummod FOXP3,expression
R15908 T21280 T21278 pobj expression,of
R15909 T21281 T21280 prep at,expression
R15910 T21282 T21284 det the,level
R15911 T21283 T21284 amod single-cell,level
R15912 T21284 T21281 pobj level,at
R15913 T21285 T21289 punct ",",stained
R15914 T21286 T21289 nsubjpass cells,stained
R15915 T21287 T21289 auxpass were,stained
R15916 T21288 T21289 advmod first,stained
R15917 T21289 T21289 ROOT stained,stained
R15918 T21290 T21289 prep with,stained
R15919 T21291 T21294 det the,CD25
R15920 T21292 T21294 amod monoclonal,CD25
R15921 T21293 T21294 compound antibody,CD25
R15922 T21294 T21290 pobj CD25,with
R15923 T21295 T21294 punct (,CD25
R15924 T21296 T21297 compound Beckman,Coulter
R15925 T21297 T21294 appos Coulter,CD25
R15926 T21298 T21294 punct ),CD25
R15927 T21299 T21289 punct ",",stained
R15928 T21300 T21289 cc and,stained
R15929 T21301 T21308 prep after,incubated
R15930 T21302 T21301 pobj fixation,after
R15931 T21303 T21302 cc and,fixation
R15932 T21304 T21302 conj permeabilization,fixation
R15933 T21305 T21308 punct ",",incubated
R15934 T21306 T21308 nsubjpass cells,incubated
R15935 T21307 T21308 auxpass were,incubated
R15936 T21308 T21289 conj incubated,stained
R15937 T21309 T21308 prep with,incubated
R15938 T21310 T21313 amod PE-conjugated,PCH101
R15939 T21311 T21312 amod monoclonal,antibody
R15940 T21312 T21313 compound antibody,PCH101
R15941 T21313 T21309 pobj PCH101,with
R15942 T21314 T21313 punct (,PCH101
R15943 T21315 T21316 amod anti-human,FOXP3
R15944 T21316 T21313 npadvmod FOXP3,PCH101
R15945 T21317 T21313 punct ;,PCH101
R15946 T21318 T21313 conj eBioscience,PCH101
R15947 T21319 T21308 punct ),incubated
R15948 T21320 T21308 prep based,incubated
R15949 T21321 T21320 prep on,based
R15950 T21322 T21323 det the,manufacturer
R15951 T21323 T21325 poss manufacturer,recommendations
R15952 T21324 T21323 case 's,manufacturer
R15953 T21325 T21321 pobj recommendations,on
R15954 T21326 T21320 cc and,based
R15955 T21327 T21320 conj subjected,based
R15956 T21328 T21327 prep to,subjected
R15957 T21329 T21328 pobj FACS,to
R15958 T21330 T21332 punct (,XL-MCL
R15959 T21331 T21332 compound EPICS,XL-MCL
R15960 T21332 T21329 appos XL-MCL,FACS
R15961 T21333 T21332 punct ),XL-MCL
R15962 T21334 T21308 punct .,incubated
R15963 T21335 T21343 prep For,incubated
R15964 T21336 T21337 compound cell,surface
R15965 T21337 T21338 compound surface,marker
R15966 T21338 T21339 compound marker,staining
R15967 T21339 T21335 pobj staining,For
R15968 T21340 T21343 punct ",",incubated
R15969 T21341 T21343 nsubjpass cells,incubated
R15970 T21342 T21343 auxpass were,incubated
R15971 T21343 T21343 ROOT incubated,incubated
R15972 T21344 T21343 prep for,incubated
R15973 T21345 T21346 nummod 20,min
R15974 T21346 T21344 pobj min,for
R15975 T21347 T21343 prep at,incubated
R15976 T21348 T21349 nummod 4,°
R15977 T21349 T21347 pobj °,at
R15978 T21350 T21343 dobj C,incubated
R15979 T21351 T21343 prep in,incubated
R15980 T21352 T21351 pcomp staining,in
R15981 T21353 T21352 dobj buffer,staining
R15982 T21354 T21352 prep with,staining
R15983 T21355 T21357 det the,antibodies
R15984 T21356 T21357 amod following,antibodies
R15985 T21357 T21354 pobj antibodies,with
R15986 T21358 T21357 punct :,antibodies
R15987 T21359 T21357 appos anti,antibodies
R15988 T21360 T21357 appos CD152-PE,antibodies
R15989 T21361 T21362 punct (,CTLA-4
R15990 T21362 T21360 appos CTLA-4,CD152-PE
R15991 T21363 T21364 punct ;,BD
R15992 T21364 T21343 npadvmod BD,incubated
R15993 T21365 T21364 punct ),BD
R15994 T21366 T21364 punct ",",BD
R15995 T21367 T21368 amod anti,PD-1
R15996 T21368 T21364 appos PD-1,BD
R15997 T21369 T21370 punct (,eBiosciences
R15998 T21370 T21368 appos eBiosciences,PD-1
R15999 T21371 T21368 punct ),PD-1
R16000 T21372 T21368 punct ",",PD-1
R16001 T21373 T21375 amod anti-GITR,R
R16002 T21374 T21375 punct (,R
R16003 T21375 T21364 appos R,BD
R16004 T21376 T21375 cc &,R
R16005 T21377 T21378 compound D,Systems
R16006 T21378 T21375 conj Systems,R
R16007 T21379 T21375 punct ),R
R16008 T21380 T21375 punct ",",R
R16009 T21381 T21375 appos anti-CD69,R
R16010 T21382 T21384 punct (,Coulter
R16011 T21383 T21384 compound Beckman,Coulter
R16012 T21384 T21381 appos Coulter,anti-CD69
R16013 T21385 T21384 punct ),Coulter
R16014 T21386 T21381 punct ",",anti-CD69
R16015 T21387 T21381 appos anti-CD103,anti-CD69
R16016 T21388 T21389 punct (,DakoCytomation
R16017 T21389 T21387 appos DakoCytomation,anti-CD103
R16018 T21390 T21389 punct ),DakoCytomation
R16019 T21391 T21387 punct ",",anti-CD103
R16020 T21392 T21387 conj anti-CD62L,anti-CD103
R16021 T21393 T21395 punct (,Coulter
R16022 T21394 T21395 compound Beckman,Coulter
R16023 T21395 T21392 appos Coulter,anti-CD62L
R16024 T21396 T21395 punct ),Coulter
R16025 T21397 T21395 punct ",",Coulter
R16026 T21398 T21395 cc or,Coulter
R16027 T21399 T21395 conj anti-HLA-DR,Coulter
R16028 T21400 T21402 punct (,Coulter
R16029 T21401 T21402 compound Beckman,Coulter
R16030 T21402 T21399 appos Coulter,anti-HLA-DR
R16031 T21403 T21399 punct ),anti-HLA-DR
R16032 T21404 T21343 punct .,incubated
R16033 T21405 T21406 det The,controls
R16034 T21406 T21407 nsubj controls,were
R16035 T21407 T21407 ROOT were,were
R16036 T21408 T21407 attr FITC,were
R16037 T21409 T21408 punct ",",FITC
R16038 T21410 T21408 conj PE,FITC
R16039 T21411 T21410 punct ",",PE
R16040 T21412 T21410 cc or,PE
R16041 T21413 T21414 compound ECD-conjugated,mouse
R16042 T21414 T21415 compound mouse,IgG1
R16043 T21415 T21410 conj IgG1,PE
R16044 T21416 T21415 cc or,IgG1
R16045 T21417 T21415 conj rat,IgG1
R16046 T21418 T21408 appos IgG2a,FITC
R16047 T21419 T21407 punct .,were
R16048 T21420 T21433 prep For,used
R16049 T21421 T21420 pobj staining,For
R16050 T21422 T21421 prep of,staining
R16051 T21423 T21424 compound mouse,cells
R16052 T21424 T21422 pobj cells,of
R16053 T21425 T21433 punct ",",used
R16054 T21426 T21428 det the,mAbs
R16055 T21427 T21428 amod following,mAbs
R16056 T21428 T21433 nsubjpass mAbs,used
R16057 T21429 T21428 prep from,mAbs
R16058 T21430 T21431 compound BD,Biosciences
R16059 T21431 T21429 pobj Biosciences,from
R16060 T21432 T21433 auxpass were,used
R16061 T21433 T21433 ROOT used,used
R16062 T21434 T21433 prep following,used
R16063 T21435 T21436 compound standard,techniques
R16064 T21436 T21434 pobj techniques,following
R16065 T21437 T21438 mark as,described
R16066 T21438 T21438 ROOT described,described
R16067 T21439 T21438 prep above,described
R16068 T21440 T21438 punct :,described
R16069 T21441 T21433 dobj anti-CD3,used
R16070 T21442 T21441 punct ",",anti-CD3
R16071 T21443 T21441 conj anti-CD4,anti-CD3
R16072 T21444 T21443 punct ",",anti-CD4
R16073 T21445 T21443 cc and,anti-CD4
R16074 T21446 T21443 conj anti-CD25,anti-CD4
R16075 T21447 T21433 punct .,used
R16076 T21448 T21451 compound Anti-FcγRII,antibody
R16077 T21449 T21450 compound /,III
R16078 T21450 T21451 compound III,antibody
R16079 T21451 T21459 nsubjpass antibody,included
R16080 T21452 T21454 punct (,G2
R16081 T21453 T21454 nummod 2.4,G2
R16082 T21454 T21451 appos G2,antibody
R16083 T21455 T21454 punct ",",G2
R16084 T21456 T21454 appos ATCC,G2
R16085 T21457 T21454 punct ),G2
R16086 T21458 T21459 auxpass was,included
R16087 T21459 T21459 ROOT included,included
R16088 T21460 T21459 prep in,included
R16089 T21461 T21462 det all,stainings
R16090 T21462 T21460 pobj stainings,in
R16091 T21463 T21464 aux to,reduce
R16092 T21464 T21459 advcl reduce,included
R16093 T21465 T21467 amod nonspecific,binding
R16094 T21466 T21467 compound antibody,binding
R16095 T21467 T21464 dobj binding,reduce
R16096 T21468 T21459 punct .,included
R16097 T21469 T21470 aux To,isolate
R16098 T21470 T21487 advcl isolate,stained
R16099 T21471 T21472 amod naive,murine
R16100 T21472 T21470 dobj murine,isolate
R16101 T21473 T21475 compound CD4,cells
R16102 T21474 T21475 compound T,cells
R16103 T21475 T21472 appos cells,murine
R16104 T21476 T21475 prep from,cells
R16105 T21477 T21478 compound murine,DO11
R16106 T21478 T21476 pobj DO11,from
R16107 T21479 T21478 nummod .10,DO11
R16108 T21480 T21478 cc or,DO11
R16109 T21481 T21483 amod DO11.10xCD2-GATA3,cells
R16110 T21482 T21483 compound T,cells
R16111 T21483 T21478 conj cells,DO11
R16112 T21484 T21475 punct ",",cells
R16113 T21485 T21487 nsubjpass cells,stained
R16114 T21486 T21487 auxpass were,stained
R16115 T21487 T21487 ROOT stained,stained
R16116 T21488 T21487 prep with,stained
R16117 T21489 T21495 amod anti-CD25-FITC,prior
R16118 T21490 T21489 punct ",",anti-CD25-FITC
R16119 T21491 T21489 conj anti-CD62L-PE,anti-CD25-FITC
R16120 T21492 T21491 punct ",",anti-CD62L-PE
R16121 T21493 T21491 cc and,anti-CD62L-PE
R16122 T21494 T21491 conj anti-CD4-APC,anti-CD62L-PE
R16123 T21495 T21487 advmod prior,stained
R16124 T21496 T21495 prep to,prior
R16125 T21497 T21496 pobj sorting,to
R16126 T21498 T21487 punct .,stained
R16127 T21499 T21500 compound Dead,cells
R16128 T21500 T21502 nsubjpass cells,excluded
R16129 T21501 T21502 auxpass were,excluded
R16130 T21502 T21509 ccomp excluded,Diamidino-2-phenylindole
R16131 T21503 T21502 prep with,excluded
R16132 T21504 T21505 nummod 4,′
R16133 T21505 T21503 pobj ′,with
R16134 T21506 T21505 punct ',′
R16135 T21507 T21509 punct ",6",Diamidino-2-phenylindole
R16136 T21508 T21509 punct -,Diamidino-2-phenylindole
R16137 T21509 T21509 ROOT Diamidino-2-phenylindole,Diamidino-2-phenylindole
R16138 T21510 T21511 punct (,DAPI
R16139 T21511 T21509 appos DAPI,Diamidino-2-phenylindole
R16140 T21512 T21511 punct ),DAPI
R16141 T21513 T21509 punct .,Diamidino-2-phenylindole
R16142 T21514 T21515 aux To,analyze
R16143 T21515 T21526 advcl analyze,stained
R16144 T21516 T21518 amod murine,expression
R16145 T21517 T21518 amod Foxp3,expression
R16146 T21518 T21515 dobj expression,analyze
R16147 T21519 T21518 prep in,expression
R16148 T21520 T21522 amod inducible,cultures
R16149 T21521 T21522 compound Treg,cultures
R16150 T21522 T21519 pobj cultures,in
R16151 T21523 T21526 punct ",",stained
R16152 T21524 T21526 nsubjpass cells,stained
R16153 T21525 T21526 auxpass were,stained
R16154 T21526 T21526 ROOT stained,stained
R16155 T21527 T21526 advmod intracellularly,stained
R16156 T21528 T21526 prep with,stained
R16157 T21529 T21530 amod anti-Foxp3-PE,according
R16158 T21530 T21526 prep according,stained
R16159 T21531 T21530 prep to,according
R16160 T21532 T21534 poss manufacturer,instruction
R16161 T21533 T21532 case 's,manufacturer
R16162 T21534 T21531 pobj instruction,to
R16163 T21535 T21526 punct ",",stained
R16164 T21536 T21526 prep in,stained
R16165 T21537 T21536 pobj conjunction,in
R16166 T21538 T21537 prep with,conjunction
R16167 T21539 T21546 amod anti-CD4-APC,kit
R16168 T21540 T21539 cc and,anti-CD4-APC
R16169 T21541 T21539 conj LIVE/DEAD,anti-CD4-APC
R16170 T21542 T21546 amod fixable,kit
R16171 T21543 T21544 amod dead,cell
R16172 T21544 T21545 compound cell,stain
R16173 T21545 T21546 compound stain,kit
R16174 T21546 T21538 pobj kit,with
R16175 T21547 T21546 punct (,kit
R16176 T21548 T21546 appos Invitrogen,kit
R16177 T21549 T21546 punct ),kit
R16178 T21550 T21551 aux to,discriminate
R16179 T21551 T21526 xcomp discriminate,stained
R16180 T21552 T21553 amod live,cells
R16181 T21553 T21551 dobj cells,discriminate
R16182 T21554 T21526 punct .,stained
R16183 T21555 T21557 det All,antibodies
R16184 T21556 T21557 amod monoclonal,antibodies
R16185 T21557 T21563 nsubjpass antibodies,purchased
R16186 T21558 T21557 prep for,antibodies
R16187 T21559 T21560 amod murine,cell
R16188 T21560 T21561 compound cell,stainings
R16189 T21561 T21558 pobj stainings,for
R16190 T21562 T21563 auxpass were,purchased
R16191 T21563 T21563 ROOT purchased,purchased
R16192 T21564 T21563 prep from,purchased
R16193 T21565 T21564 pobj eBioscience,from
R16194 T21566 T21565 cc or,eBioscience
R16195 T21567 T21568 compound BD,Biosciences
R16196 T21568 T21565 conj Biosciences,eBioscience
R16197 T21569 T21563 punct .,purchased
R16383 T21972 T21973 amod Western,blotting
R16384 T21973 T21973 ROOT blotting,blotting
R16385 T21974 T21973 punct .,blotting
R16386 T21975 T21991 prep For,lysed
R16387 T21976 T21977 nummod FOXP3,analysis
R16388 T21977 T21975 pobj analysis,For
R16389 T21978 T21977 prep on,analysis
R16390 T21979 T21981 det the,level
R16391 T21980 T21981 compound protein,level
R16392 T21981 T21978 pobj level,on
R16393 T21982 T21991 punct ",",lysed
R16394 T21983 T21985 nummod 1,106
R16395 T21984 T21985 nummod ×,106
R16396 T21985 T21988 nummod 106,CD25
R16397 T21986 T21988 compound CD4,CD25
R16398 T21987 T21988 compound +,CD25
R16399 T21988 T21991 npadvmod CD25,lysed
R16400 T21989 T21991 nsubjpass cells,lysed
R16401 T21990 T21991 auxpass were,lysed
R16402 T21991 T21991 ROOT lysed,lysed
R16403 T21992 T21991 cc and,lysed
R16404 T21993 T21991 conj loaded,lysed
R16405 T21994 T21993 advmod next,loaded
R16406 T21995 T21994 prep to,next
R16407 T21996 T21998 det a,ladder
R16408 T21997 T21998 amod protein-mass,ladder
R16409 T21998 T21995 pobj ladder,to
R16410 T21999 T21998 punct (,ladder
R16411 T22000 T21998 appos Magicmark,ladder
R16412 T22001 T22000 punct ",",Magicmark
R16413 T22002 T22000 appos Invitrogen,Magicmark
R16414 T22003 T22000 punct ),Magicmark
R16415 T22004 T21998 prep on,ladder
R16416 T22005 T22011 det a,gel
R16417 T22006 T22011 nmod NuPAGE,gel
R16418 T22007 T22006 nummod 4,NuPAGE
R16419 T22008 T22009 nummod 12,%
R16420 T22009 T22011 nmod %,gel
R16421 T22010 T22011 amod bis-tris,gel
R16422 T22011 T22004 pobj gel,on
R16423 T22012 T22013 punct (,Invitrogen
R16424 T22013 T22011 appos Invitrogen,gel
R16425 T22014 T21993 punct ),loaded
R16426 T22015 T21991 punct .,lysed
R16427 T22016 T22017 det The,proteins
R16428 T22017 T22019 nsubjpass proteins,electroblotted
R16429 T22018 T22019 auxpass were,electroblotted
R16430 T22019 T22019 ROOT electroblotted,electroblotted
R16431 T22020 T22019 prep onto,electroblotted
R16432 T22021 T22023 det a,membrane
R16433 T22022 T22023 compound PVDF,membrane
R16434 T22023 T22020 pobj membrane,onto
R16435 T22024 T22027 punct (,Science
R16436 T22025 T22027 compound Amersham,Science
R16437 T22026 T22027 compound Life,Science
R16438 T22027 T22023 appos Science,membrane
R16439 T22028 T22019 punct ),electroblotted
R16440 T22029 T22019 punct .,electroblotted
R16441 T22030 T22031 amod Unspecific,binding
R16442 T22031 T22033 nsubjpass binding,blocked
R16443 T22032 T22033 auxpass was,blocked
R16444 T22033 T22033 ROOT blocked,blocked
R16445 T22034 T22033 prep with,blocked
R16446 T22035 T22034 pobj BSA,with
R16447 T22036 T22033 punct ",",blocked
R16448 T22037 T22033 cc and,blocked
R16449 T22038 T22039 det the,membranes
R16450 T22039 T22042 nsubjpass membranes,incubated
R16451 T22040 T22042 auxpass were,incubated
R16452 T22041 T22042 advmod subsequently,incubated
R16453 T22042 T22033 conj incubated,blocked
R16454 T22043 T22042 prep with,incubated
R16455 T22044 T22046 det an,dilution
R16456 T22045 T22046 nummod 1:200,dilution
R16457 T22046 T22043 pobj dilution,with
R16458 T22047 T22046 prep of,dilution
R16459 T22048 T22049 compound goat,anti-FOXP3
R16460 T22049 T22047 pobj anti-FOXP3,of
R16461 T22050 T22046 prep in,dilution
R16462 T22051 T22050 pcomp blocking,in
R16463 T22052 T22051 dobj buffer,blocking
R16464 T22053 T22052 punct (,buffer
R16465 T22054 T22052 appos Abcam,buffer
R16466 T22055 T22052 punct ),buffer
R16467 T22056 T22051 npadvmod overnight,blocking
R16468 T22057 T22051 prep at,blocking
R16469 T22058 T22059 nummod 4,°
R16470 T22059 T22057 pobj °,at
R16471 T22060 T22050 pobj C,in
R16472 T22061 T22042 punct .,incubated
R16473 T22062 T22063 det The,blots
R16474 T22063 T22065 nsubjpass blots,developed
R16475 T22064 T22065 auxpass were,developed
R16476 T22065 T22065 ROOT developed,developed
R16477 T22066 T22065 advcl using,developed
R16478 T22067 T22070 det an,mAb
R16479 T22068 T22070 amod anti-goat,mAb
R16480 T22069 T22070 compound HRP-labeled,mAb
R16481 T22070 T22066 dobj mAb,using
R16482 T22071 T22073 punct (,Biosciences
R16483 T22072 T22073 compound Amersham,Biosciences
R16484 T22073 T22070 parataxis Biosciences,mAb
R16485 T22074 T22073 punct ),Biosciences
R16486 T22075 T22066 cc and,using
R16487 T22076 T22066 conj visualized,using
R16488 T22077 T22076 prep with,visualized
R16489 T22078 T22081 det a,camera
R16490 T22079 T22081 nmod LAS,camera
R16491 T22080 T22081 nummod 1000,camera
R16492 T22081 T22077 pobj camera,with
R16493 T22082 T22081 punct (,camera
R16494 T22083 T22081 appos Fuji,camera
R16495 T22084 T22081 punct ),camera
R16496 T22085 T22065 punct .,developed
R16497 T22086 T22088 nsubjpass Membranes,incubated
R16498 T22087 T22088 auxpass were,incubated
R16499 T22088 T22088 ROOT incubated,incubated
R16500 T22089 T22088 prep in,incubated
R16501 T22090 T22089 pcomp stripping,in
R16502 T22091 T22094 meta buffer,for
R16503 T22092 T22091 cc and,buffer
R16504 T22093 T22091 conj re-blocked,buffer
R16505 T22094 T22090 prep for,stripping
R16506 T22095 T22096 nummod 1,h
R16507 T22096 T22094 pobj h,for
R16508 T22097 T22088 punct .,incubated
R16509 T22098 T22099 det The,membranes
R16510 T22099 T22100 nsubj membranes,were
R16511 T22100 T22100 ROOT were,were
R16512 T22101 T22110 dep re-probed,Biotechnology
R16513 T22102 T22101 advcl using,re-probed
R16514 T22103 T22102 dobj anti-GATA3,using
R16515 T22104 T22103 punct (,anti-GATA3
R16516 T22105 T22103 appos HG3,anti-GATA3
R16517 T22106 T22103 nummod 31,anti-GATA3
R16518 T22107 T22110 punct ;,Biotechnology
R16519 T22108 T22109 compound Santa,Cruz
R16520 T22109 T22110 compound Cruz,Biotechnology
R16521 T22110 T22100 acomp Biotechnology,were
R16522 T22111 T22110 punct ),Biotechnology
R16523 T22112 T22110 punct ",",Biotechnology
R16524 T22113 T22110 conj anti-T-bet,Biotechnology
R16525 T22114 T22129 punct (,anti-phospho-SMAD2
R16526 T22115 T22129 nmod 4B10,anti-phospho-SMAD2
R16527 T22116 T22115 punct ",",4B10
R16528 T22117 T22118 compound Santa,Cruz
R16529 T22118 T22119 compound Cruz,Biotechnology
R16530 T22119 T22115 appos Biotechnology,4B10
R16531 T22120 T22115 punct ),4B10
R16532 T22121 T22115 punct ",",4B10
R16533 T22122 T22115 conj anti-GAPDH,4B10
R16534 T22123 T22122 punct (,anti-GAPDH
R16535 T22124 T22122 appos 6C5,anti-GAPDH
R16536 T22125 T22122 punct ",",anti-GAPDH
R16537 T22126 T22122 appos Ambion,anti-GAPDH
R16538 T22127 T22122 punct ),anti-GAPDH
R16539 T22128 T22122 punct ",",anti-GAPDH
R16540 T22129 T22113 appos anti-phospho-SMAD2,anti-T-bet
R16541 T22130 T22129 punct (,anti-phospho-SMAD2
R16542 T22131 T22129 appos 138D4,anti-phospho-SMAD2
R16543 T22132 T22129 punct ),anti-phospho-SMAD2
R16544 T22133 T22129 punct ",",anti-phospho-SMAD2
R16545 T22134 T22129 appos anti-phospho-STAT6,anti-phospho-SMAD2
R16546 T22135 T22136 punct (,5A4
R16547 T22136 T22134 appos 5A4,anti-phospho-STAT6
R16548 T22137 T22136 punct ),5A4
R16549 T22138 T22134 punct ",",anti-phospho-STAT6
R16550 T22139 T22134 cc and,anti-phospho-STAT6
R16551 T22140 T22134 conj anti-STAT6,anti-phospho-STAT6
R16552 T22141 T22144 punct (,Technology
R16553 T22142 T22144 compound Cell,Technology
R16554 T22143 T22144 compound Signaling,Technology
R16555 T22144 T22140 parataxis Technology,anti-STAT6
R16556 T22145 T22144 punct ),Technology
R16557 T22146 T22110 punct ",",Biotechnology
R16673 T22376 T22376 ROOT Administration,Administration
R16674 T22377 T22376 prep of,Administration
R16675 T22378 T22377 pobj cytokines,of
R16676 T22379 T22378 cc and,cytokines
R16677 T22380 T22378 conj antibodies,cytokines
R16678 T22381 T22378 prep in,cytokines
R16679 T22382 T22381 pobj vivo,in
R16680 T22383 T22376 punct .,Administration
R16681 T22384 T22391 nsubj Age,received
R16682 T22385 T22384 punct -,Age
R16683 T22386 T22384 cc and,Age
R16684 T22387 T22390 amod gender-matched,mice
R16685 T22388 T22390 amod normal,mice
R16686 T22389 T22390 compound B6,mice
R16687 T22390 T22384 conj mice,Age
R16688 T22391 T22391 ROOT received,received
R16689 T22392 T22394 det every,day
R16690 T22393 T22394 amod other,day
R16691 T22394 T22397 nmod day,ip
R16692 T22395 T22397 nmod intraperitoneal,ip
R16693 T22396 T22397 punct (,ip
R16694 T22397 T22391 dobj ip,received
R16695 T22398 T22397 punct ),ip
R16696 T22399 T22391 dobj injections,received
R16697 T22400 T22399 prep of,injections
R16698 T22401 T22400 pobj PBS,of
R16699 T22402 T22399 punct ",",injections
R16700 T22403 T22405 nummod 1.5,rmIL-4
R16701 T22404 T22405 compound μg,rmIL-4
R16702 T22405 T22399 appos rmIL-4,injections
R16703 T22406 T22405 punct ",",rmIL-4
R16704 T22407 T22408 nummod 50,μg
R16705 T22408 T22410 compound μg,mAb
R16706 T22409 T22410 compound anti-IL-4,mAb
R16707 T22410 T22405 appos mAb,rmIL-4
R16708 T22411 T22410 punct (,mAb
R16709 T22412 T22410 appos 11B11,mAb
R16710 T22413 T22412 cc or,11B11
R16711 T22414 T22412 conj MAB404,11B11
R16712 T22415 T22410 punct ),mAb
R16713 T22416 T22410 punct ",",mAb
R16714 T22417 T22405 cc or,rmIL-4
R16715 T22418 T22419 det a,mixture
R16716 T22419 T22405 conj mixture,rmIL-4
R16717 T22420 T22419 prep of,mixture
R16718 T22421 T22423 nummod 1.5,rmIL-4
R16719 T22422 T22423 compound μg,rmIL-4
R16720 T22423 T22420 pobj rmIL-4,of
R16721 T22424 T22419 cc plus,mixture
R16722 T22425 T22426 nummod 50,μg
R16723 T22426 T22428 compound μg,mAb
R16724 T22427 T22428 compound anti-IL-4,mAb
R16725 T22428 T22419 appos mAb,mixture
R16726 T22429 T22430 punct (,11B11
R16727 T22430 T22428 appos 11B11,mAb
R16728 T22431 T22430 cc or,11B11
R16729 T22432 T22430 conj MAB404,11B11
R16730 T22433 T22428 punct ),mAb
R16731 T22434 T22405 prep for,rmIL-4
R16732 T22435 T22436 nummod 7,d.
R16733 T22436 T22434 pobj d.,for
R16734 T22437 T22445 advmod Thereafter,analyzed
R16735 T22438 T22445 punct ",",analyzed
R16736 T22439 T22443 amod spleen,cells
R16737 T22440 T22439 cc and,spleen
R16738 T22441 T22439 conj lymph,spleen
R16739 T22442 T22443 compound node,cells
R16740 T22443 T22445 nsubjpass cells,analyzed
R16741 T22444 T22445 auxpass were,analyzed
R16742 T22445 T22391 advcl analyzed,received
R16743 T22446 T22445 agent by,analyzed
R16744 T22447 T22448 compound flow,cytometry
R16745 T22448 T22446 pobj cytometry,by
R16746 T22449 T22445 prep for,analyzed
R16747 T22450 T22449 pobj CD3,for
R16748 T22451 T22450 punct ",",CD3
R16749 T22452 T22450 conj CD4,CD3
R16750 T22453 T22445 punct ",",analyzed
R16751 T22454 T22445 cc and,analyzed
R16752 T22455 T22456 compound CD25,expression
R16753 T22456 T22445 conj expression,analyzed
R16754 T22457 T22391 punct .,received
R16755 T22458 T22460 det The,IL-4
R16756 T22459 T22460 amod anti-mouse,IL-4
R16757 T22460 T22464 nsubjpass IL-4,obtained
R16758 T22461 T22462 compound mAb,MAB404
R16759 T22462 T22460 appos MAB404,IL-4
R16760 T22463 T22464 auxpass was,obtained
R16761 T22464 T22464 ROOT obtained,obtained
R16762 T22465 T22464 prep from,obtained
R16763 T22466 T22467 compound R&D,Systems
R16764 T22467 T22465 pobj Systems,from
R16765 T22468 T22467 punct ",",Systems
R16766 T22469 T22472 det the,IL-4
R16767 T22470 T22472 amod second,IL-4
R16768 T22471 T22472 amod anti-mouse,IL-4
R16769 T22472 T22467 appos IL-4,Systems
R16770 T22473 T22474 compound mAb,11B11
R16771 T22474 T22476 nsubjpass 11B11,purchased
R16772 T22475 T22476 auxpass was,purchased
R16773 T22476 T22464 conj purchased,obtained
R16774 T22477 T22476 prep from,purchased
R16775 T22478 T22477 pobj eBioscience,from
R16776 T22479 T22464 punct .,obtained
R16968 T22754 T22754 ROOT ChIP,ChIP
R16969 T22755 T22754 punct .,ChIP
R16970 T22756 T22757 compound ChIP,analysis
R16971 T22757 T22759 nsubjpass analysis,performed
R16972 T22758 T22759 auxpass was,performed
R16973 T22759 T22759 ROOT performed,performed
R16974 T22760 T22759 prep according,performed
R16975 T22761 T22760 prep to,according
R16976 T22762 T22763 det the,manufacturer
R16977 T22763 T22765 poss manufacturer,protocol
R16978 T22764 T22763 case 's,manufacturer
R16979 T22765 T22761 pobj protocol,to
R16980 T22766 T22768 punct (,Biotechnology
R16981 T22767 T22768 compound Upstate,Biotechnology
R16982 T22768 T22765 appos Biotechnology,protocol
R16983 T22769 T22759 punct ),performed
R16984 T22770 T22759 prep with,performed
R16985 T22771 T22773 det the,modifications
R16986 T22772 T22773 amod following,modifications
R16987 T22773 T22770 pobj modifications,with
R16988 T22774 T22759 punct .,performed
R16989 T22775 T22778 nmod iTreg,cells
R16990 T22776 T22775 cc and,iTreg
R16991 T22777 T22775 conj Th2,iTreg
R16992 T22778 T22780 nsubjpass cells,fixed
R16993 T22779 T22780 auxpass were,fixed
R16994 T22780 T22780 ROOT fixed,fixed
R16995 T22781 T22780 prep with,fixed
R16996 T22782 T22783 nummod 1,%
R16997 T22783 T22784 compound %,formaldehyde
R16998 T22784 T22781 pobj formaldehyde,with
R16999 T22785 T22780 prep for,fixed
R17000 T22786 T22787 nummod 10,min
R17001 T22787 T22785 pobj min,for
R17002 T22788 T22787 prep at,min
R17003 T22789 T22790 compound room,temperature
R17004 T22790 T22788 pobj temperature,at
R17005 T22791 T22780 punct .,fixed
R17006 T22792 T22793 det The,chromatin
R17007 T22793 T22795 nsubjpass chromatin,sheared
R17008 T22794 T22795 auxpass was,sheared
R17009 T22795 T22795 ROOT sheared,sheared
R17010 T22796 T22795 prep to,sheared
R17011 T22797 T22798 nummod 200-1000,bp
R17012 T22798 T22796 pobj bp,to
R17013 T22799 T22798 prep of,bp
R17014 T22800 T22799 pobj length,of
R17015 T22801 T22795 agent by,sheared
R17016 T22802 T22801 pobj sonication,by
R17017 T22803 T22795 prep with,sheared
R17018 T22804 T22805 nummod five,pulses
R17019 T22805 T22803 pobj pulses,with
R17020 T22806 T22805 prep of,pulses
R17021 T22807 T22806 pobj 10,of
R17022 T22808 T22807 npadvmod s,10
R17023 T22809 T22805 prep at,pulses
R17024 T22810 T22811 nummod 30,%
R17025 T22811 T22812 compound %,power
R17026 T22812 T22809 pobj power,at
R17027 T22813 T22814 punct (,Bandelin
R17028 T22814 T22812 appos Bandelin,power
R17029 T22815 T22814 punct ),Bandelin
R17030 T22816 T22795 punct .,sheared
R17031 T22817 T22818 det The,chromatin
R17032 T22818 T22819 nsubj chromatin,was
R17033 T22819 T22851 ccomp was,used
R17034 T22820 T22819 acomp pre-cleared,was
R17035 T22821 T22820 prep for,pre-cleared
R17036 T22822 T22823 nummod 2,h
R17037 T22823 T22821 pobj h,for
R17038 T22824 T22823 prep with,h
R17039 T22825 T22826 amod normal,mouse
R17040 T22826 T22828 compound mouse,beads
R17041 T22827 T22828 compound IgG,beads
R17042 T22828 T22824 pobj beads,with
R17043 T22829 T22819 cc and,was
R17044 T22830 T22831 advmod then,incubated
R17045 T22831 T22819 conj incubated,was
R17046 T22832 T22831 prep with,incubated
R17047 T22833 T22834 amod anti-GATA3-agarose,beads
R17048 T22834 T22832 pobj beads,with
R17049 T22835 T22834 punct (,beads
R17050 T22836 T22834 appos HG3,beads
R17051 T22837 T22834 appos 31,beads
R17052 T22838 T22851 punct ;,used
R17053 T22839 T22840 compound Santa,Cruz
R17054 T22840 T22841 compound Cruz,Biotechnology
R17055 T22841 T22851 nsubjpass Biotechnology,used
R17056 T22842 T22841 punct ),Biotechnology
R17057 T22843 T22841 prep for,Biotechnology
R17058 T22844 T22845 nummod 2,h.
R17059 T22845 T22843 pobj h.,for
R17060 T22846 T22845 acl Washing,h.
R17061 T22847 T22846 cc and,Washing
R17062 T22848 T22849 compound elution,buffers
R17063 T22849 T22851 nsubjpass buffers,used
R17064 T22850 T22851 auxpass were,used
R17065 T22851 T22851 ROOT used,used
R17066 T22852 T22851 prep according,used
R17067 T22853 T22852 prep to,according
R17068 T22854 T22855 det the,protocol
R17069 T22855 T22853 pobj protocol,to
R17070 T22856 T22855 prep of,protocol
R17071 T22857 T22858 compound Upstate,Biotechnology
R17072 T22858 T22856 pobj Biotechnology,of
R17073 T22859 T22851 punct .,used
R17074 T22860 T22862 nsubjpass Crosslinks,reversed
R17075 T22861 T22862 auxpass were,reversed
R17076 T22862 T22862 ROOT reversed,reversed
R17077 T22863 T22862 agent by,reversed
R17078 T22864 T22863 pobj incubation,by
R17079 T22865 T22862 prep at,reversed
R17080 T22866 T22868 nummod 65,C
R17081 T22867 T22868 nummod °,C
R17082 T22868 T22865 pobj C,at
R17083 T22869 T22862 prep for,reversed
R17084 T22870 T22871 nummod 4,h
R17085 T22871 T22869 pobj h,for
R17086 T22872 T22862 prep in,reversed
R17087 T22873 T22874 det the,presence
R17088 T22874 T22872 pobj presence,in
R17089 T22875 T22874 prep of,presence
R17090 T22876 T22878 nummod 0.2,NaCl
R17091 T22877 T22878 compound M,NaCl
R17092 T22878 T22875 pobj NaCl,of
R17093 T22879 T22862 punct ",",reversed
R17094 T22880 T22862 cc and,reversed
R17095 T22881 T22882 det the,DNA
R17096 T22882 T22884 nsubjpass DNA,purified
R17097 T22883 T22884 auxpass was,purified
R17098 T22884 T22862 conj purified,reversed
R17099 T22885 T22884 agent by,purified
R17100 T22886 T22887 compound phenol/chloroform,extraction
R17101 T22887 T22885 pobj extraction,by
R17102 T22888 T22862 punct .,reversed
R17103 T22889 T22890 det The,amount
R17104 T22890 T22894 nsubjpass amount,determined
R17105 T22891 T22890 prep of,amount
R17106 T22892 T22891 pobj DNA,of
R17107 T22893 T22894 auxpass was,determined
R17108 T22894 T22894 ROOT determined,determined
R17109 T22895 T22894 agent by,determined
R17110 T22896 T22897 amod conventional,PCR
R17111 T22897 T22895 pobj PCR,by
R17112 T22898 T22894 punct .,determined
R17113 T22899 T22900 det The,PCR
R17114 T22900 T22901 nsubj PCR,addressed
R17115 T22901 T22901 ROOT addressed,addressed
R17116 T22902 T22901 prep for,addressed
R17117 T22903 T22906 det the,region
R17118 T22904 T22906 amod FOXP3,region
R17119 T22905 T22906 compound promoter,region
R17120 T22906 T22902 pobj region,for
R17121 T22907 T22901 dobj −,addressed
R17122 T22908 T22907 nummod 246,−
R17123 T22909 T22908 prep to,246
R17124 T22910 T22909 pobj −,to
R17125 T22911 T22910 appos 511,−
R17126 T22912 T22901 cc and,addressed
R17127 T22913 T22914 auxpass was,performed
R17128 T22914 T22901 conj performed,addressed
R17129 T22915 T22914 xcomp using,performed
R17130 T22916 T22918 det the,primers
R17131 T22917 T22918 amod following,primers
R17132 T22918 T22915 dobj primers,using
R17133 T22919 T22918 punct :,primers
R17134 T22920 T22925 nummod 5,′
R17135 T22921 T22923 punct ′,gtgccctttacgagt
R17136 T22922 T22923 punct -,gtgccctttacgagt
R17137 T22923 T22925 nmod gtgccctttacgagt,′
R17138 T22924 T22925 amod catctg-3,′
R17139 T22925 T22918 appos ′,primers
R17140 T22926 T22925 cc and,′
R17141 T22927 T22930 nummod 5,gtgccctttacgagtcatctg-3
R17142 T22928 T22930 punct ′,gtgccctttacgagtcatctg-3
R17143 T22929 T22930 punct -,gtgccctttacgagtcatctg-3
R17144 T22930 T22931 amod gtgccctttacgagtcatctg-3,′
R17145 T22931 T22925 conj ′,′
R17146 T22932 T22901 punct .,addressed
R17147 T22933 T22935 det The,products
R17148 T22934 T22935 compound PCR,products
R17149 T22935 T22937 nsubjpass products,visualized
R17150 T22936 T22937 auxpass were,visualized
R17151 T22937 T22937 ROOT visualized,visualized
R17152 T22938 T22937 advcl using,visualized
R17153 T22939 T22942 det an,gel
R17154 T22940 T22942 amod ethidium,gel
R17155 T22941 T22942 compound bromide,gel
R17156 T22942 T22938 dobj gel,using
R17157 T22943 T22937 punct .,visualized
R17371 T23192 T23193 amod Pull-down,assay
R17372 T23193 T23193 ROOT assay,assay
R17373 T23194 T23193 punct .,assay
R17374 T23195 T23198 nummod CD4,cells
R17375 T23196 T23198 nummod +,cells
R17376 T23197 T23198 compound T,cells
R17377 T23198 T23200 nsubjpass cells,stimulated
R17378 T23199 T23200 auxpass were,stimulated
R17379 T23200 T23200 ROOT stimulated,stimulated
R17380 T23201 T23200 prep with,stimulated
R17381 T23202 T23201 pobj PMA,with
R17382 T23203 T23202 cc and,PMA
R17383 T23204 T23202 conj ionomycin,PMA
R17384 T23205 T23200 prep for,stimulated
R17385 T23206 T23207 nummod 2,h
R17386 T23207 T23205 pobj h,for
R17387 T23208 T23200 prep at,stimulated
R17388 T23209 T23210 nummod 37,°
R17389 T23210 T23211 nummod °,C
R17390 T23211 T23208 pobj C,at
R17391 T23212 T23200 punct .,stimulated
R17392 T23213 T23214 det The,cells
R17393 T23214 T23216 nsubjpass cells,pelleted
R17394 T23215 T23216 auxpass were,pelleted
R17395 T23216 T23216 ROOT pelleted,pelleted
R17396 T23217 T23216 punct ",",pelleted
R17397 T23218 T23216 conj resuspended,pelleted
R17398 T23219 T23218 prep in,resuspended
R17399 T23220 T23221 compound buffer,C
R17400 T23221 T23219 pobj C,in
R17401 T23222 T23229 punct (,]
R17402 T23223 T23229 nummod 20,]
R17403 T23224 T23227 compound mM,pH
R17404 T23225 T23227 compound HEPES,pH
R17405 T23226 T23227 compound [,pH
R17406 T23227 T23229 compound pH,]
R17407 T23228 T23229 compound 7.9,]
R17408 T23229 T23221 appos ],C
R17409 T23230 T23229 punct ",",]
R17410 T23231 T23233 nummod 420,NaCl
R17411 T23232 T23233 compound mM,NaCl
R17412 T23233 T23229 appos NaCl,]
R17413 T23234 T23233 punct ",",NaCl
R17414 T23235 T23237 nummod 1.5,MgCl2
R17415 T23236 T23237 compound mM,MgCl2
R17416 T23237 T23233 appos MgCl2,NaCl
R17417 T23238 T23237 punct ",",MgCl2
R17418 T23239 T23241 nummod 0.2,EDTA
R17419 T23240 T23241 compound mM,EDTA
R17420 T23241 T23237 appos EDTA,MgCl2
R17421 T23242 T23241 punct ",",EDTA
R17422 T23243 T23245 nummod 1,DTT
R17423 T23244 T23245 compound mM,DTT
R17424 T23245 T23241 appos DTT,EDTA
R17425 T23246 T23245 punct ",",DTT
R17426 T23247 T23248 compound protease,inhibitors
R17427 T23248 T23259 nsubj inhibitors,lysed
R17428 T23249 T23251 compound [,]
R17429 T23250 T23251 compound Sigma,]
R17430 T23251 T23248 appos ],inhibitors
R17431 T23252 T23251 punct .,]
R17432 T23253 T23248 cc and,inhibitors
R17433 T23254 T23255 nummod 0.1,%
R17434 T23255 T23256 compound %,NP-40
R17435 T23256 T23248 conj NP-40,inhibitors
R17436 T23257 T23256 punct ),NP-40
R17437 T23258 T23256 cc and,NP-40
R17438 T23259 T23218 ccomp lysed,resuspended
R17439 T23260 T23259 prep on,lysed
R17440 T23261 T23260 pobj ice,on
R17441 T23262 T23259 prep for,lysed
R17442 T23263 T23264 nummod 15,min
R17443 T23264 T23262 pobj min,for
R17444 T23265 T23216 punct .,pelleted
R17445 T23266 T23267 amod Insoluble,material
R17446 T23267 T23269 nsubjpass material,removed
R17447 T23268 T23269 auxpass was,removed
R17448 T23269 T23269 ROOT removed,removed
R17449 T23270 T23269 agent by,removed
R17450 T23271 T23270 pobj centrifugation,by
R17451 T23272 T23269 punct .,removed
R17452 T23273 T23274 det The,supernatant
R17453 T23274 T23276 nsubjpass supernatant,diluted
R17454 T23275 T23276 auxpass was,diluted
R17455 T23276 T23276 ROOT diluted,diluted
R17456 T23277 T23276 oprd 1:3,diluted
R17457 T23278 T23277 prep with,1:3
R17458 T23279 T23280 compound buffer,D
R17459 T23280 T23278 pobj D,with
R17460 T23281 T23282 punct (,as
R17461 T23282 T23280 prep as,D
R17462 T23283 T23284 compound buffer,C
R17463 T23284 T23282 pobj C,as
R17464 T23285 T23276 punct ",",diluted
R17465 T23286 T23276 cc but,diluted
R17466 T23287 T23286 prep without,but
R17467 T23288 T23287 pobj NaCl,without
R17468 T23289 T23276 punct ),diluted
R17469 T23290 T23276 punct .,diluted
R17470 T23291 T23292 det The,lysates
R17471 T23292 T23294 nsubjpass lysates,incubated
R17472 T23293 T23294 auxpass were,incubated
R17473 T23294 T23294 ROOT incubated,incubated
R17474 T23295 T23294 prep with,incubated
R17475 T23296 T23297 nummod 10,μg
R17476 T23297 T23295 pobj μg,with
R17477 T23298 T23297 prep of,μg
R17478 T23299 T23298 pobj poly,of
R17479 T23300 T23301 punct (,dI-dC
R17480 T23301 T23299 appos dI-dC,poly
R17481 T23302 T23294 punct ),incubated
R17482 T23303 T23294 punct (,incubated
R17483 T23304 T23304 ROOT Sigma,Sigma
R17484 T23305 T23304 punct ),Sigma
R17485 T23306 T23294 cc and,incubated
R17486 T23307 T23308 nummod 70,μl
R17487 T23308 T23294 conj μl,incubated
R17488 T23309 T23308 prep of,μl
R17489 T23310 T23309 pobj streptavidin-agarose,of
R17490 T23311 T23313 punct (,Biosciences
R17491 T23312 T23313 compound Amersham,Biosciences
R17492 T23313 T23310 appos Biosciences,streptavidin-agarose
R17493 T23314 T23313 punct ),Biosciences
R17494 T23315 T23308 acl carrying,μl
R17495 T23316 T23317 amod biotinylated,oligonucleotides
R17496 T23317 T23315 dobj oligonucleotides,carrying
R17497 T23318 T23315 punct ",",carrying
R17498 T23319 T23308 prep for,μl
R17499 T23320 T23321 nummod 3,h
R17500 T23321 T23319 pobj h,for
R17501 T23322 T23308 prep at,μl
R17502 T23323 T23324 nummod 4,°
R17503 T23324 T23322 pobj °,at
R17504 T23325 T23308 npadvmod C,μl
R17505 T23326 T23294 punct .,incubated
R17506 T23327 T23328 det The,beads
R17507 T23328 T23330 nsubjpass beads,washed
R17508 T23329 T23330 auxpass were,washed
R17509 T23330 T23351 ccomp washed,heated
R17510 T23331 T23330 advmod twice,washed
R17511 T23332 T23330 prep with,washed
R17512 T23333 T23335 compound buffer,:D
R17513 T23334 T23335 compound C,:D
R17514 T23335 T23332 pobj :D,with
R17515 T23336 T23335 punct (,:D
R17516 T23337 T23335 appos 1:3,:D
R17517 T23338 T23335 punct ),:D
R17518 T23339 T23330 cc and,washed
R17519 T23340 T23330 conj resuspended,washed
R17520 T23341 T23340 prep in,resuspended
R17521 T23342 T23344 amod DTT-containing,buffer
R17522 T23343 T23344 compound loading,buffer
R17523 T23344 T23341 pobj buffer,in
R17524 T23345 T23346 punct (,NuPAGE
R17525 T23346 T23344 appos NuPAGE,buffer
R17526 T23347 T23351 punct ;,heated
R17527 T23348 T23351 nsubj Invitrogen,heated
R17528 T23349 T23348 punct ),Invitrogen
R17529 T23350 T23351 punct ",",heated
R17530 T23351 T23351 ROOT heated,heated
R17531 T23352 T23351 prep to,heated
R17532 T23353 T23354 compound 70,°
R17533 T23354 T23355 compound °,C
R17534 T23355 T23352 pobj C,to
R17535 T23356 T23351 prep for,heated
R17536 T23357 T23358 nummod 10,min
R17537 T23358 T23356 pobj min,for
R17538 T23359 T23351 punct ",",heated
R17539 T23360 T23351 cc and,heated
R17540 T23361 T23362 det the,eluants
R17541 T23362 T23351 conj eluants,heated
R17542 T23363 T23362 prep on,eluants
R17543 T23364 T23370 det a,gel
R17544 T23365 T23370 nmod NuPAGE,gel
R17545 T23366 T23365 nummod 4,NuPAGE
R17546 T23367 T23368 nummod 12,%
R17547 T23368 T23370 nmod %,gel
R17548 T23369 T23370 amod bis-tris,gel
R17549 T23370 T23363 pobj gel,on
R17550 T23371 T23372 punct (,Invitrogen
R17551 T23372 T23370 appos Invitrogen,gel
R17552 T23373 T23362 punct ),eluants
R17553 T23374 T23351 punct .,heated
R17554 T23375 T23376 det The,proteins
R17555 T23376 T23378 nsubjpass proteins,electroblotted
R17556 T23377 T23378 auxpass were,electroblotted
R17557 T23378 T23378 ROOT electroblotted,electroblotted
R17558 T23379 T23378 prep onto,electroblotted
R17559 T23380 T23382 det a,membrane
R17560 T23381 T23382 compound PVDF,membrane
R17561 T23382 T23379 pobj membrane,onto
R17562 T23383 T23385 punct (,Biosciences
R17563 T23384 T23385 compound Amersham,Biosciences
R17564 T23385 T23382 appos Biosciences,membrane
R17565 T23386 T23378 punct ),electroblotted
R17566 T23387 T23378 cc and,electroblotted
R17567 T23388 T23378 conj detected,electroblotted
R17568 T23389 T23388 xcomp using,detected
R17569 T23390 T23392 det an,mAb
R17570 T23391 T23392 compound anti-GATA3,mAb
R17571 T23392 T23389 dobj mAb,using
R17572 T23393 T23392 punct (,mAb
R17573 T23394 T23395 compound Santa,Cruz
R17574 T23395 T23396 compound Cruz,Biotechnology
R17575 T23396 T23392 appos Biotechnology,mAb
R17576 T23397 T23392 punct ),mAb
R17577 T23398 T23378 punct .,electroblotted
R17578 T23399 T23400 amod Accumulated,signals
R17579 T23400 T23402 nsubjpass signals,analyzed
R17580 T23401 T23402 auxpass were,analyzed
R17581 T23402 T23402 ROOT analyzed,analyzed
R17582 T23403 T23402 advcl using,analyzed
R17583 T23404 T23405 compound AIDA,software
R17584 T23405 T23403 dobj software,using
R17585 T23406 T23405 punct (,software
R17586 T23407 T23405 appos Raytest,software
R17587 T23408 T23405 punct ),software
R17588 T23409 T23402 punct .,analyzed
R17704 T23591 T23595 npadvmod Th2,Induce
R17705 T23592 T23595 nsubj Cells,Induce
R17706 T23593 T23595 aux Can,Induce
R17707 T23594 T23595 neg not,Induce
R17708 T23595 T23595 ROOT Induce,Induce
R17709 T23596 T23597 compound FOXP3,Expression
R17710 T23597 T23595 dobj Expression,Induce
R17711 T23598 T23597 punct (,Expression
R17712 T23599 T23597 appos A,Expression
R17713 T23600 T23599 punct ),A
R17714 T23601 T23602 compound Human,T
R17715 T23602 T23603 compound T,cells
R17716 T23603 T23605 nsubjpass cells,activated
R17717 T23604 T23605 auxpass were,activated
R17718 T23605 T23595 ccomp activated,Induce
R17719 T23606 T23605 prep with,activated
R17720 T23607 T23609 nmod plate-bound,/
R17721 T23608 T23609 amod anti-CD3,/
R17722 T23609 T23606 pobj /,with
R17723 T23610 T23609 nummod CD28,/
R17724 T23611 T23606 conj with,with
R17725 T23612 T23611 cc or,with
R17726 T23613 T23611 conj without,with
R17727 T23614 T23613 pobj TGF-β,without
R17728 T23615 T23616 mark as,indicated
R17729 T23616 T23605 advcl indicated,activated
R17730 T23617 T23616 prep on,indicated
R17731 T23618 T23619 det the,x-axis
R17732 T23619 T23617 pobj x-axis,on
R17733 T23620 T23619 prep of,x-axis
R17734 T23621 T23622 punct (,B
R17735 T23622 T23620 pobj B,of
R17736 T23623 T23622 punct ),B
R17737 T23624 T23595 punct .,Induce
R17738 T23625 T23627 nsubjpass Cells,harvested
R17739 T23626 T23627 auxpass were,harvested
R17740 T23627 T23627 ROOT harvested,harvested
R17741 T23628 T23627 prep after,harvested
R17742 T23629 T23630 nummod 5,days
R17743 T23630 T23628 pobj days,after
R17744 T23631 T23630 cc and,days
R17745 T23632 T23633 nummod FOXP3,mRNA
R17746 T23633 T23630 conj mRNA,days
R17747 T23634 T23635 auxpass was,quantified
R17748 T23635 T23627 conj quantified,harvested
R17749 T23636 T23635 agent by,quantified
R17750 T23637 T23638 amod real-time,PCR
R17751 T23638 T23636 pobj PCR,by
R17752 T23639 T23627 punct .,harvested
R17753 T23640 T23641 nsubj Bars,show
R17754 T23641 T23641 ROOT show,show
R17755 T23642 T23645 det the,SD
R17756 T23643 T23645 amod mean,SD
R17757 T23644 T23645 compound ±,SD
R17758 T23645 T23641 dobj SD,show
R17759 T23646 T23645 prep of,SD
R17760 T23647 T23649 nummod 4,experiments
R17761 T23648 T23649 amod independent,experiments
R17762 T23649 T23646 pobj experiments,of
R17763 T23650 T23641 punct .,show
R17764 T23651 T23652 punct (,B
R17765 T23652 T23652 ROOT B,B
R17766 T23653 T23652 punct ),B
R17767 T23654 T23665 prep In,activated
R17768 T23655 T23654 pobj vitro,In
R17769 T23656 T23657 amod differentiated,Th1
R17770 T23657 T23663 nmod Th1,cells
R17771 T23658 T23659 punct ",",Th2
R17772 T23659 T23657 conj Th2,Th1
R17773 T23660 T23659 punct ",",Th2
R17774 T23661 T23659 cc or,Th2
R17775 T23662 T23659 conj iTreg,Th2
R17776 T23663 T23665 nsubjpass cells,activated
R17777 T23664 T23665 auxpass were,activated
R17778 T23665 T23665 ROOT activated,activated
R17779 T23666 T23665 prep with,activated
R17780 T23667 T23669 amod anti-CD3,CD28
R17781 T23668 T23669 compound /,CD28
R17782 T23669 T23666 pobj CD28,with
R17783 T23670 T23669 punct ",",CD28
R17784 T23671 T23669 conj TGF-β,CD28
R17785 T23672 T23671 punct ",",TGF-β
R17786 T23673 T23671 cc or,TGF-β
R17787 T23674 T23671 conj anti-IL-4,TGF-β
R17788 T23675 T23676 mark as,indicated
R17789 T23676 T23665 advcl indicated,activated
R17790 T23677 T23665 punct .,activated
R17791 T23678 T23679 det The,phenotype
R17792 T23679 T23684 nsubjpass phenotype,confirmed
R17793 T23680 T23679 prep of,phenotype
R17794 T23681 T23682 det these,cells
R17795 T23682 T23680 pobj cells,of
R17796 T23683 T23684 auxpass was,confirmed
R17797 T23684 T23684 ROOT confirmed,confirmed
R17798 T23685 T23684 agent by,confirmed
R17799 T23686 T23685 pobj FACS,by
R17800 T23687 T23686 cc and,FACS
R17801 T23688 T23689 compound proliferation,analysis
R17802 T23689 T23686 conj analysis,FACS
R17803 T23690 T23691 punct (,Figure
R17804 T23691 T23689 appos Figure,analysis
R17805 T23692 T23691 nummod S1,Figure
R17806 T23693 T23691 punct ),Figure
R17807 T23694 T23684 punct .,confirmed
R17808 T23695 T23696 nsubj Bars,show
R17809 T23696 T23696 ROOT show,show
R17810 T23697 T23700 det the,SD
R17811 T23698 T23700 amod mean,SD
R17812 T23699 T23700 compound ±,SD
R17813 T23700 T23696 dobj SD,show
R17814 T23701 T23700 prep of,SD
R17815 T23702 T23704 nummod four,experiments
R17816 T23703 T23704 amod independent,experiments
R17817 T23704 T23701 pobj experiments,of
R17818 T23705 T23696 punct .,show
R17929 T23907 T23908 nummod Th2,Cells
R17930 T23908 T23908 ROOT Cells,Cells
R17931 T23909 T23912 nmod Do,FOXP3
R17932 T23910 T23912 neg Not,FOXP3
R17933 T23911 T23912 compound Express,FOXP3
R17934 T23912 T23908 appos FOXP3,Cells
R17935 T23913 T23914 punct (,A
R17936 T23914 T23912 appos A,FOXP3
R17937 T23915 T23912 punct ),FOXP3
R17938 T23916 T23917 compound Intracellular,FACS
R17939 T23917 T23918 compound FACS,analysis
R17940 T23918 T23935 nsubj analysis,see
R17941 T23919 T23918 prep of,analysis
R17942 T23920 T23921 nummod FOXP3,expression
R17943 T23921 T23919 pobj expression,of
R17944 T23922 T23921 prep in,expression
R17945 T23923 T23922 pobj Th1,in
R17946 T23924 T23923 punct ",",Th1
R17947 T23925 T23923 conj Th2,Th1
R17948 T23926 T23925 punct ",",Th2
R17949 T23927 T23925 cc or,Th2
R17950 T23928 T23929 amod iTreg-differentiated,cells
R17951 T23929 T23925 conj cells,Th2
R17952 T23930 T23929 punct (,cells
R17953 T23931 T23932 nummod two,rounds
R17954 T23932 T23929 conj rounds,cells
R17955 T23933 T23932 punct ",",rounds
R17956 T23934 T23935 nsubj phenotype,see
R17957 T23935 T23935 ROOT see,see
R17958 T23936 T23935 dobj Figure,see
R17959 T23937 T23936 nummod S1,Figure
R17960 T23938 T23936 punct ),Figure
R17961 T23939 T23935 punct ",",see
R17962 T23940 T23935 dep rested,see
R17963 T23941 T23940 cc or,rested
R17964 T23942 T23940 conj activated,rested
R17965 T23943 T23942 punct ",",activated
R17966 T23944 T23942 prep with,activated
R17967 T23945 T23944 cc or,with
R17968 T23946 T23944 conj without,with
R17969 T23947 T23946 pobj TGF-β,without
R17970 T23948 T23935 punct .,see
R17971 T23949 T23950 nummod FOXP3,expression
R17972 T23950 T23952 nsubjpass expression,measured
R17973 T23951 T23952 auxpass was,measured
R17974 T23952 T23952 ROOT measured,measured
R17975 T23953 T23952 prep after,measured
R17976 T23954 T23955 nummod 5,d
R17977 T23955 T23953 pobj d,after
R17978 T23956 T23955 prep in,d
R17979 T23957 T23956 pobj culture,in
R17980 T23958 T23952 punct .,measured
R17981 T23959 T23961 det The,blots
R17982 T23960 T23961 amod dot,blots
R17983 T23961 T23962 nsubj blots,are
R17984 T23962 T23962 ROOT are,are
R17985 T23963 T23962 attr representative,are
R17986 T23964 T23963 prep of,representative
R17987 T23965 T23967 nummod three,experiments
R17988 T23966 T23967 amod independent,experiments
R17989 T23967 T23964 pobj experiments,of
R17990 T23968 T23962 punct .,are
R17991 T23969 T23970 punct (,B
R17992 T23970 T23972 nsubj B,Shows
R17993 T23971 T23970 punct ),B
R17994 T23972 T23972 ROOT Shows,Shows
R17995 T23973 T23976 det the,setup
R17996 T23974 T23976 amod same,setup
R17997 T23975 T23976 amod experimental,setup
R17998 T23976 T23972 dobj setup,Shows
R17999 T23977 T23972 punct ",",Shows
R18000 T23978 T23972 cc but,Shows
R18001 T23979 T23980 advmod naturally,occurring
R18002 T23980 T23972 conj occurring,Shows
R18003 T23981 T23982 amod Th2,cells
R18004 T23982 T23984 nsubjpass cells,analyzed
R18005 T23983 T23984 auxpass were,analyzed
R18006 T23984 T23980 advcl analyzed,occurring
R18007 T23985 T23972 punct .,Shows
R18008 T23986 T23987 nsubj Data,are
R18009 T23987 T23987 ROOT are,are
R18010 T23988 T23987 attr representative,are
R18011 T23989 T23988 prep of,representative
R18012 T23990 T23992 nummod three,experiments
R18013 T23991 T23992 amod independent,experiments
R18014 T23992 T23989 pobj experiments,of
R18015 T23993 T23987 punct .,are
R18208 T24303 T24303 ROOT Th2,Th2
R18209 T24304 T24303 punct -,Th2
R18210 T24305 T24303 cc or,Th2
R18211 T24306 T24315 nmod IL-4,analysis
R18212 T24307 T24308 compound Producing,Cells
R18213 T24308 T24310 compound Cells,FOXP3
R18214 T24309 T24310 compound Lack,FOXP3
R18215 T24310 T24306 appos FOXP3,IL-4
R18216 T24311 T24312 punct (,A
R18217 T24312 T24310 appos A,FOXP3
R18218 T24313 T24310 punct ),FOXP3
R18219 T24314 T24315 compound FACS,analysis
R18220 T24315 T24303 conj analysis,Th2
R18221 T24316 T24315 prep of,analysis
R18222 T24317 T24318 amod intracellular,FOXP3
R18223 T24318 T24316 pobj FOXP3,of
R18224 T24319 T24318 cc and,FOXP3
R18225 T24320 T24321 compound IL-4,expression
R18226 T24321 T24315 conj expression,analysis
R18227 T24322 T24303 prep following,Th2
R18228 T24323 T24324 compound PMA/Ionomycin,stimulation
R18229 T24324 T24322 pobj stimulation,following
R18230 T24325 T24322 punct .,following
R18231 T24326 T24329 nummod CD4,cells
R18232 T24327 T24329 nummod +,cells
R18233 T24328 T24329 compound T,cells
R18234 T24329 T24331 nsubjpass cells,gated
R18235 T24330 T24331 auxpass were,gated
R18236 T24331 T24331 ROOT gated,gated
R18237 T24332 T24331 prep on,gated
R18238 T24333 T24334 det the,basis
R18239 T24334 T24332 pobj basis,on
R18240 T24335 T24334 prep of,basis
R18241 T24336 T24335 pobj CD45RO,of
R18242 T24337 T24336 cc and,CD45RO
R18243 T24338 T24340 nummod CD25,expression
R18244 T24339 T24340 compound surface,expression
R18245 T24340 T24336 conj expression,CD45RO
R18246 T24341 T24340 punct (,expression
R18247 T24342 T24343 amod upper,panel
R18248 T24343 T24340 appos panel,expression
R18249 T24344 T24340 punct ),expression
R18250 T24345 T24331 punct ",",gated
R18251 T24346 T24331 cc and,gated
R18252 T24347 T24348 amod gated,cells
R18253 T24348 T24350 nsubjpass cells,shown
R18254 T24349 T24350 auxpass are,shown
R18255 T24350 T24331 conj shown,gated
R18256 T24351 T24350 prep below,shown
R18257 T24352 T24350 prep for,shown
R18258 T24353 T24357 det the,−
R18259 T24354 T24357 compound CD45RO,−
R18260 T24355 T24357 compound +,−
R18261 T24356 T24357 compound CD25,−
R18262 T24357 T24352 pobj −,for
R18263 T24358 T24359 punct (,A
R18264 T24359 T24357 appos A,−
R18265 T24360 T24361 punct ",",left
R18266 T24361 T24350 advcl left,shown
R18267 T24362 T24361 dobj panel,left
R18268 T24363 T24350 punct ),shown
R18269 T24364 T24381 punct ",",subsets
R18270 T24365 T24369 det the,+
R18271 T24366 T24369 compound CD45RO,+
R18272 T24367 T24369 compound +,+
R18273 T24368 T24369 compound CD25,+
R18274 T24369 T24381 nmod +,subsets
R18275 T24370 T24372 punct (,panel
R18276 T24371 T24372 amod right,panel
R18277 T24372 T24369 appos panel,+
R18278 T24373 T24372 punct ),panel
R18279 T24374 T24369 punct ",",+
R18280 T24375 T24369 cc and,+
R18281 T24376 T24379 det the,CD25
R18282 T24377 T24379 compound CD45RO,CD25
R18283 T24378 T24379 compound −,CD25
R18284 T24379 T24369 conj CD25,+
R18285 T24380 T24381 compound −,subsets
R18286 T24381 T24331 conj subsets,gated
R18287 T24382 T24381 punct (,subsets
R18288 T24383 T24384 amod central,panel
R18289 T24384 T24381 appos panel,subsets
R18290 T24385 T24381 punct ),subsets
R18291 T24386 T24381 punct .,subsets
R18292 T24387 T24389 det A,analysis
R18293 T24388 T24389 amod statistical,analysis
R18294 T24389 T24401 nsubjpass analysis,shown
R18295 T24390 T24389 prep of,analysis
R18296 T24391 T24393 nummod eight,donors
R18297 T24392 T24393 amod independent,donors
R18298 T24393 T24390 pobj donors,of
R18299 T24394 T24389 prep after,analysis
R18300 T24395 T24394 pobj subtraction,after
R18301 T24396 T24395 prep of,subtraction
R18302 T24397 T24399 det the,control
R18303 T24398 T24399 compound isotype,control
R18304 T24399 T24396 pobj control,of
R18305 T24400 T24401 auxpass are,shown
R18306 T24401 T24401 ROOT shown,shown
R18307 T24402 T24401 prep in,shown
R18308 T24403 T24404 punct (,B
R18309 T24404 T24402 pobj B,in
R18310 T24405 T24404 punct ),B
R18311 T24406 T24401 punct .,shown
R18312 T24407 T24410 det The,line
R18313 T24408 T24410 amod dotted,line
R18314 T24409 T24410 amod gray,line
R18315 T24410 T24411 nsubj line,indicates
R18316 T24411 T24411 ROOT indicates,indicates
R18317 T24412 T24415 det the,level
R18318 T24413 T24415 compound IC,level
R18319 T24414 T24415 compound background,level
R18320 T24415 T24411 dobj level,indicates
R18321 T24416 T24411 punct .,indicates
R18322 T24417 T24419 det The,bars
R18323 T24418 T24419 compound error,bars
R18324 T24419 T24420 nsubj bars,show
R18325 T24420 T24420 ROOT show,show
R18326 T24421 T24422 det the,error
R18327 T24422 T24420 dobj error,show
R18328 T24423 T24422 prep of,error
R18329 T24424 T24425 det the,mean
R18330 T24425 T24423 pobj mean,of
R18331 T24426 T24420 punct .,show
R18332 T24427 T24428 punct (,C
R18333 T24428 T24428 ROOT C,C
R18334 T24429 T24428 punct ),C
R18335 T24430 T24455 advmod Similarly,stained
R18336 T24431 T24455 punct ",",stained
R18337 T24432 T24434 det a,clone
R18338 T24433 T24434 amod Th2,clone
R18339 T24434 T24455 nsubjpass clone,stained
R18340 T24435 T24434 punct (,clone
R18341 T24436 T24434 appos BR8,clone
R18342 T24437 T24434 punct ),clone
R18343 T24438 T24434 punct ",",clone
R18344 T24439 T24442 compound CRTH2,cells
R18345 T24440 T24442 compound +,cells
R18346 T24441 T24442 compound Th2,cells
R18347 T24442 T24434 conj cells,clone
R18348 T24443 T24442 punct ",",cells
R18349 T24444 T24445 amod IL-4-secreting,cells
R18350 T24445 T24442 conj cells,cells
R18351 T24446 T24445 punct ",",cells
R18352 T24447 T24445 cc and,cells
R18353 T24448 T24450 compound memory,cells
R18354 T24449 T24450 compound T,cells
R18355 T24450 T24445 conj cells,cells
R18356 T24451 T24450 punct (,cells
R18357 T24452 T24450 appos CD45RO,cells
R18358 T24453 T24450 punct ),cells
R18359 T24454 T24455 auxpass were,stained
R18360 T24455 T24455 ROOT stained,stained
R18361 T24456 T24455 prep for,stained
R18362 T24457 T24456 pobj FOXP3,for
R18363 T24458 T24457 cc and,FOXP3
R18364 T24459 T24457 conj IL-4,FOXP3
R18365 T24460 T24455 punct .,stained
R18366 T24461 T24462 nsubj Data,are
R18367 T24462 T24462 ROOT are,are
R18368 T24463 T24462 attr representative,are
R18369 T24464 T24463 prep of,representative
R18370 T24465 T24467 nummod three,experiments
R18371 T24466 T24467 amod independent,experiments
R18372 T24467 T24464 pobj experiments,of
R18373 T24468 T24462 punct .,are
R18541 T24825 T24826 nummod FOXP3,Induction
R18542 T24826 T24842 npadvmod Induction,activated
R18543 T24827 T24826 prep During,Induction
R18544 T24828 T24830 det the,Process
R18545 T24829 T24830 compound Differentiation,Process
R18546 T24830 T24827 pobj Process,During
R18547 T24831 T24837 punct (,CD45RA
R18548 T24832 T24837 nmod A,CD45RA
R18549 T24833 T24837 punct ),CD45RA
R18550 T24834 T24835 compound Human,CD4
R18551 T24835 T24837 compound CD4,CD45RA
R18552 T24836 T24837 compound +,CD45RA
R18553 T24837 T24838 nsubj CD45RA,+
R18554 T24838 T24842 advmod +,activated
R18555 T24839 T24840 compound T,cells
R18556 T24840 T24842 nsubjpass cells,activated
R18557 T24841 T24842 auxpass were,activated
R18558 T24842 T24842 ROOT activated,activated
R18559 T24843 T24842 prep with,activated
R18560 T24844 T24846 nmod plate-bound,/
R18561 T24845 T24846 compound anti-CD3,/
R18562 T24846 T24843 pobj /,with
R18563 T24847 T24846 nummod CD28,/
R18564 T24848 T24842 prep in,activated
R18565 T24849 T24850 det the,presence
R18566 T24850 T24848 pobj presence,in
R18567 T24851 T24850 prep of,presence
R18568 T24852 T24851 pobj TGF-β,of
R18569 T24853 T24852 punct (,TGF-β
R18570 T24854 T24855 nummod 5,ng/ml
R18571 T24855 T24852 appos ng/ml,TGF-β
R18572 T24856 T24855 punct ),ng/ml
R18573 T24857 T24855 cc or,ng/ml
R18574 T24858 T24855 conj IL-4,ng/ml
R18575 T24859 T24861 punct (,ng/ml
R18576 T24860 T24861 nummod 25,ng/ml
R18577 T24861 T24858 appos ng/ml,IL-4
R18578 T24862 T24861 punct ),ng/ml
R18579 T24863 T24842 punct .,activated
R18590 T24874 T24876 nsubjpass mRNA,quantified
R18591 T24875 T24876 auxpass was,quantified
R18592 T24876 T24867 conj quantified,harvested
R18593 T24877 T24876 agent by,quantified
R18594 T24878 T24879 amod real-time,PCR
R18595 T24879 T24877 pobj PCR,by
R18596 T24880 T24876 prep for,quantified
R18597 T24881 T24880 pobj FOXP3,for
R18598 T24882 T24881 cc and,FOXP3
R18599 T24883 T24884 compound GATA3,expression
R18600 T24884 T24881 conj expression,FOXP3
R18601 T24885 T24876 punct .,quantified
R18602 T24886 T24887 nsubj Bars,show
R18603 T24887 T24887 ROOT show,show
R18604 T24888 T24891 det the,SD
R18605 T24889 T24891 amod mean,SD
R18606 T24890 T24891 compound ±,SD
R18607 T24891 T24887 dobj SD,show
R18608 T24892 T24891 prep of,SD
R18609 T24893 T24895 nummod three,experiments
R18610 T24894 T24895 amod independent,experiments
R18611 T24895 T24892 pobj experiments,of
R18612 T24896 T24887 punct .,show
R18613 T24897 T24906 punct (,shown
R18614 T24898 T24906 dep B,shown
R18615 T24899 T24898 punct ),B
R18616 T24900 T24901 compound Intracellular,GATA3
R18617 T24901 T24898 appos GATA3,B
R18618 T24902 T24901 cc and,GATA3
R18619 T24903 T24901 conj FOXP3,GATA3
R18620 T24904 T24906 nsubjpass staining,shown
R18621 T24905 T24906 auxpass is,shown
R18622 T24906 T24906 ROOT shown,shown
R18623 T24907 T24917 mark after,differentiating
R18624 T24908 T24907 pobj exposure,after
R18625 T24909 T24908 prep of,exposure
R18626 T24910 T24912 compound CD4,CD45RA
R18627 T24911 T24912 compound +,CD45RA
R18628 T24912 T24909 pobj CD45RA,of
R18629 T24913 T24915 amod +,cells
R18630 T24914 T24915 compound T,cells
R18631 T24915 T24917 nsubj cells,differentiating
R18632 T24916 T24917 aux to,differentiating
R18633 T24917 T24906 advcl differentiating,shown
R18634 T24918 T24917 dobj conditions,differentiating
R18635 T24919 T24917 prep as,differentiating
R18636 T24920 T24919 prep in,as
R18648 T24932 T24933 amod independent,experiments
R18649 T24933 T24930 pobj experiments,of
R18650 T24934 T24928 punct .,are
R18910 T25390 T25390 ROOT Effect,Effect
R18911 T25391 T25390 prep of,Effect
R18912 T25392 T25391 pobj IL-4,of
R18913 T25393 T25390 prep on,Effect
R18914 T25394 T25395 compound FOXP3,Induction
R18915 T25395 T25393 pobj Induction,on
R18916 T25396 T25395 punct (,Induction
R18917 T25397 T25395 appos A,Induction
R18918 T25398 T25395 punct ),Induction
R18919 T25399 T25401 det A,analysis
R18920 T25400 T25401 amod statistical,analysis
R18921 T25401 T25403 nsubjpass analysis,performed
R18922 T25402 T25403 auxpass was,performed
R18923 T25403 T25428 ccomp performed,is
R18924 T25404 T25403 prep with,performed
R18925 T25405 T25406 nummod six,donors
R18926 T25406 T25404 pobj donors,with
R18927 T25407 T25403 prep on,performed
R18928 T25408 T25407 pobj day,on
R18929 T25409 T25408 nummod 5,day
R18930 T25410 T25417 punct (,with
R18931 T25411 T25417 amod TGF-β,with
R18932 T25412 T25414 punct (,ng/ml
R18933 T25413 T25414 nummod 10,ng/ml
R18934 T25414 T25411 appos ng/ml,TGF-β
R18935 T25415 T25414 punct ),ng/ml
R18936 T25416 T25411 cc and,TGF-β
R18937 T25417 T25403 prep with,performed
R18938 T25418 T25417 cc or,with
R18939 T25419 T25417 conj without,with
R18940 T25420 T25419 pobj IL-4,without
R18941 T25421 T25420 punct (,IL-4
R18942 T25422 T25423 nummod 100,ng/ml
R18943 T25423 T25420 appos ng/ml,IL-4
R18944 T25424 T25420 punct ),IL-4
R18945 T25425 T25417 punct ),with
R18946 T25426 T25428 punct ;,is
R18947 T25427 T25428 advmod Shown,is
R18948 T25428 T25428 ROOT is,is
R18949 T25429 T25430 det the,mean
R18950 T25430 T25428 attr mean,is
R18951 T25431 T25430 punct ",",mean
R18952 T25432 T25430 cc and,mean
R18953 T25433 T25434 compound error,bars
R18954 T25434 T25435 nsubj bars,indicated
R18955 T25435 T25428 conj indicated,is
R18956 T25436 T25437 det the,SD
R18957 T25437 T25435 dobj SD,indicated
R18958 T25438 T25437 prep of,SD
R18959 T25439 T25440 nummod six,donors
R18960 T25440 T25438 pobj donors,of
R18961 T25441 T25435 punct .,indicated
R18962 T25442 T25443 compound Statistical,analysis
R18963 T25443 T25445 nsubjpass analysis,performed
R18964 T25444 T25445 auxpass was,performed
R18965 T25445 T25445 ROOT performed,performed
R18966 T25446 T25445 advcl using,performed
R18967 T25447 T25449 det the,test
R18968 T25448 T25449 compound Dunnett,test
R18969 T25449 T25446 dobj test,using
R18970 T25450 T25445 punct .,performed
R18971 T25451 T25452 compound Statistical,significance
R18972 T25452 T25454 nsubjpass significance,indicated
R18973 T25453 T25454 auxpass is,indicated
R18974 T25454 T25454 ROOT indicated,indicated
R18975 T25455 T25454 agent by,indicated
R18976 T25456 T25455 pobj asterisks,by
R18977 T25457 T25461 punct (,0.05
R18978 T25458 T25461 punct *,0.05
R18979 T25459 T25461 amod p,0.05
R18980 T25460 T25461 compound ≤,0.05
R18981 T25461 T25456 appos 0.05,asterisks
R18982 T25462 T25461 punct ",",0.05
R18983 T25463 T25466 punct **,0.01
R18984 T25464 T25466 amod p,0.01
R18985 T25465 T25466 compound ≤,0.01
R18986 T25466 T25461 appos 0.01,0.05
R18987 T25467 T25461 punct ",",0.05
R18988 T25468 T25461 appos Dunnett,0.05
R18989 T25469 T25461 punct ),0.05
R18990 T25470 T25454 punct .,indicated
R18991 T25471 T25480 punct (,activated
R18992 T25472 T25476 nmod B,CD45RA
R18993 T25473 T25472 punct ),B
R18994 T25474 T25476 compound CD4,CD45RA
R18995 T25475 T25476 compound +,CD45RA
R18996 T25476 T25480 nsubjpass CD45RA,activated
R18997 T25477 T25478 amod +,cells
R18998 T25478 T25480 nsubjpass cells,activated
R18999 T25479 T25480 auxpass were,activated
R19000 T25480 T25480 ROOT activated,activated
R19001 T25481 T25480 prep in,activated
R19002 T25482 T25483 det the,presence
R19003 T25483 T25481 pobj presence,in
R19004 T25484 T25483 prep of,presence
R19005 T25485 T25487 det a,concentration
R19006 T25486 T25487 amod constant,concentration
R19007 T25487 T25484 pobj concentration,of
R19008 T25488 T25487 prep of,concentration
R19009 T25489 T25488 pobj TGF-β,of
R19010 T25490 T25492 punct (,ng/ml
R19011 T25491 T25492 nummod 5,ng/ml
R19012 T25492 T25489 appos ng/ml,TGF-β
R19013 T25493 T25489 punct ),TGF-β
R19014 T25494 T25487 prep with,concentration
R19015 T25495 T25497 det an,concentration
R19016 T25496 T25497 amod increasing,concentration
R19017 T25497 T25494 pobj concentration,with
R19018 T25498 T25497 prep of,concentration
R19019 T25499 T25498 pobj IL-4,of
R19020 T25500 T25480 punct ",",activated
R19021 T25501 T25502 mark as,indicated
R19022 T25502 T25480 advcl indicated,activated
R19023 T25503 T25480 punct .,activated
R19024 T25504 T25506 nsubjpass Cells,harvested
R19025 T25505 T25506 auxpass were,harvested
R19026 T25506 T25506 ROOT harvested,harvested
R19027 T25507 T25506 prep for,harvested
R19028 T25508 T25509 compound mRNA,quantification
R19029 T25509 T25507 pobj quantification,for
R19030 T25510 T25506 prep after,harvested
R19031 T25511 T25512 nummod 5,d.
R19032 T25512 T25510 pobj d.,after
R19033 T25513 T25512 punct (,d.
R19034 T25514 T25512 npadvmod C,d.
R19035 T25515 T25514 punct ),C
R19036 T25516 T25518 compound CD4,CD45RA
R19037 T25517 T25518 compound +,CD45RA
R19038 T25518 T25522 nsubjpass CD45RA,stimulated
R19039 T25519 T25520 amod +,cells
R19040 T25520 T25522 nsubjpass cells,stimulated
R19041 T25521 T25522 auxpass were,stimulated
R19042 T25522 T25522 ROOT stimulated,stimulated
R19043 T25523 T25522 prep in,stimulated
R19044 T25524 T25523 pobj vitro,in
R19045 T25525 T25524 prep with,vitro
R19046 T25526 T25529 amod plate-bound,CD28
R19047 T25527 T25528 amod anti-CD3,/
R19048 T25528 T25529 compound /,CD28
R19049 T25529 T25525 pobj CD28,with
R19050 T25530 T25529 punct ",",CD28
R19051 T25531 T25529 conj TGF-β,CD28
R19052 T25532 T25531 punct (,TGF-β
R19053 T25533 T25531 parataxis 10ng/ml,TGF-β
R19054 T25534 T25531 punct ),TGF-β
R19055 T25535 T25522 punct ",",stimulated
R19056 T25536 T25522 cc and,stimulated
R19057 T25537 T25522 conj IL-4,stimulated
R19058 T25538 T25540 punct (,ng/ml
R19059 T25539 T25540 nummod 100,ng/ml
R19060 T25540 T25537 appos ng/ml,IL-4
R19061 T25541 T25537 punct ),IL-4
R19062 T25542 T25543 mark as,indicated
R19063 T25543 T25522 conj indicated,stimulated
R19064 T25544 T25522 punct .,stimulated
R19065 T25545 T25552 prep After,prepared
R19066 T25546 T25547 nummod 1,h
R19067 T25547 T25545 pobj h,After
R19068 T25548 T25552 punct ",",prepared
R19069 T25549 T25550 compound cell,lysates
R19070 T25550 T25552 nsubjpass lysates,prepared
R19071 T25551 T25552 auxpass were,prepared
R19072 T25552 T25552 ROOT prepared,prepared
R19073 T25553 T25552 cc and,prepared
R19074 T25554 T25552 conj analyzed,prepared
R19075 T25555 T25554 agent by,analyzed
R19076 T25556 T25557 amod Western,blot
R19077 T25557 T25555 pobj blot,by
R19078 T25558 T25554 prep for,analyzed
R19079 T25559 T25560 compound phosphorylated,SMAD2
R19080 T25560 T25558 pobj SMAD2,for
R19081 T25561 T25560 cc and,SMAD2
R19082 T25562 T25560 conj STAT6,SMAD2
R19083 T25563 T25552 punct .,prepared
R19084 T25564 T25565 compound Total,STAT6
R19085 T25565 T25568 nsubj STAT6,served
R19086 T25566 T25565 cc and,STAT6
R19087 T25567 T25565 conj GAPDH,STAT6
R19088 T25568 T25568 ROOT served,served
R19089 T25569 T25568 prep as,served
R19090 T25570 T25571 amod internal,control
R19091 T25571 T25569 pobj control,as
R19092 T25572 T25568 punct .,served
R19093 T25573 T25582 punct (,shown
R19094 T25574 T25577 nmod D,GATA3
R19095 T25575 T25574 punct ),D
R19096 T25576 T25577 compound Intracellular,GATA3
R19097 T25577 T25582 nsubjpass GATA3,shown
R19098 T25578 T25577 cc and,GATA3
R19099 T25579 T25577 conj FOXP3,GATA3
R19100 T25580 T25582 nsubjpass staining,shown
R19101 T25581 T25582 auxpass are,shown
R19102 T25582 T25582 ROOT shown,shown
R19103 T25583 T25600 prep after,are
R19104 T25584 T25583 pobj exposure,after
R19105 T25585 T25584 prep of,exposure
R19106 T25586 T25588 compound CD4,CD45RA
R19107 T25587 T25588 compound +,CD45RA
R19108 T25588 T25585 pobj CD45RA,of
R19109 T25589 T25600 advcl +,are
R19110 T25590 T25591 compound T,cells
R19111 T25591 T25589 dobj cells,+
R19112 T25592 T25595 aux to,described
R19113 T25593 T25592 pobj IL-4,to
R19114 T25594 T25595 mark as,described
R19115 T25595 T25600 advcl described,are
R19116 T25596 T25595 prep for,described
R19117 T25597 T25599 compound panel,Data
R19118 T25598 T25599 compound B.,Data
R19119 T25599 T25596 pobj Data,for
R19120 T25600 T25582 ccomp are,shown
R19121 T25601 T25600 acomp representative,are
R19122 T25602 T25601 prep of,representative
R19123 T25603 T25605 nummod three,experiments
R19124 T25604 T25605 amod independent,experiments
R19125 T25605 T25602 pobj experiments,of
R19126 T25606 T25600 punct .,are
R19297 T26012 T26013 nsubj IL-4,Inhibits
R19298 T26013 T26013 ROOT Inhibits,Inhibits
R19299 T26014 T26013 dobj TGF-β,Inhibits
R19300 T26015 T26017 compound Mediated,Commitment
R19301 T26016 T26017 compound iTreg,Commitment
R19302 T26017 T26018 compound Commitment,CFSE-labeled
R19303 T26018 T26021 compound CFSE-labeled,CD45RA
R19304 T26019 T26021 compound CD4,CD45RA
R19305 T26020 T26021 compound +,CD45RA
R19306 T26021 T26014 appos CD45RA,TGF-β
R19307 T26022 T26023 amod +,cells
R19308 T26023 T26025 nsubjpass cells,activated
R19309 T26024 T26025 auxpass were,activated
R19310 T26025 T26013 advcl activated,Inhibits
R19311 T26026 T26025 prep with,activated
R19312 T26027 T26030 amod plate-bound,CD28
R19313 T26028 T26029 amod anti-CD3,/
R19314 T26029 T26030 compound /,CD28
R19315 T26030 T26026 pobj CD28,with
R19316 T26031 T26030 punct ",",CD28
R19317 T26032 T26030 conj TGF-β,CD28
R19318 T26033 T26032 punct ",",TGF-β
R19319 T26034 T26032 cc and,TGF-β
R19320 T26035 T26032 conj IL-4,TGF-β
R19321 T26036 T26025 punct ",",activated
R19322 T26037 T26038 mark as,indicated
R19323 T26038 T26025 advcl indicated,activated
R19324 T26039 T26013 punct .,Inhibits
R19325 T26040 T26046 mark After,analyzed
R19326 T26041 T26046 nsubjpass 5,analyzed
R19327 T26042 T26041 advmod d,5
R19328 T26043 T26046 punct ",",analyzed
R19329 T26044 T26046 nsubjpass cells,analyzed
R19330 T26045 T26046 auxpass were,analyzed
R19331 T26046 T26060 advcl analyzed,shown
R19332 T26047 T26046 agent by,analyzed
R19333 T26048 T26049 compound flow,cytometry
R19334 T26049 T26047 pobj cytometry,by
R19335 T26050 T26051 punct (,A
R19336 T26051 T26049 parataxis A,cytometry
R19337 T26052 T26051 punct ),A
R19338 T26053 T26049 cc and,cytometry
R19339 T26054 T26060 nsubjpass results,shown
R19340 T26055 T26054 prep of,results
R19341 T26056 T26058 nummod six,experiments
R19342 T26057 T26058 amod independent,experiments
R19343 T26058 T26055 pobj experiments,of
R19344 T26059 T26060 auxpass are,shown
R19345 T26060 T26060 ROOT shown,shown
R19346 T26061 T26060 prep in,shown
R19347 T26062 T26064 det the,graph
R19348 T26063 T26064 compound bar,graph
R19349 T26064 T26061 pobj graph,in
R19350 T26065 T26064 prep below,graph
R19351 T26066 T26067 punct (,B
R19352 T26067 T26065 pobj B,below
R19353 T26068 T26067 punct ),B
R19354 T26069 T26060 punct .,shown
R19355 T26070 T26071 compound Statistical,significance
R19356 T26071 T26079 nsubjpass significance,indicated
R19357 T26072 T26074 punct (,Anova
R19358 T26073 T26074 amod one-way,Anova
R19359 T26074 T26071 appos Anova,significance
R19360 T26075 T26074 punct ",",Anova
R19361 T26076 T26074 appos Newman-Keuls,Anova
R19362 T26077 T26074 punct ),Anova
R19363 T26078 T26079 auxpass is,indicated
R19364 T26079 T26079 ROOT indicated,indicated
R19365 T26080 T26079 agent by,indicated
R19366 T26081 T26080 pobj asterisks,by
R19367 T26082 T26086 punct (,0.01
R19368 T26083 T26086 punct **,0.01
R19369 T26084 T26086 amod p,0.01
R19370 T26085 T26086 compound ≤,0.01
R19371 T26086 T26081 appos 0.01,asterisks
R19372 T26087 T26086 punct ",",0.01
R19373 T26088 T26090 dep ***,≤
R19374 T26089 T26090 dep p,≤
R19375 T26090 T26086 dep ≤,0.01
R19376 T26091 T26090 pobj 0.001,≤
R19377 T26092 T26086 punct ),0.01
R19378 T26093 T26079 punct .,indicated
R19379 T26094 T26095 punct (,C
R19380 T26095 T26098 nmod C,analysis
R19381 T26096 T26095 punct ),C
R19382 T26097 T26098 compound Kinetic,analysis
R19383 T26098 T26106 nsubjpass analysis,shown
R19384 T26099 T26098 prep of,analysis
R19385 T26100 T26101 compound intracellular,GATA3
R19386 T26101 T26099 pobj GATA3,of
R19387 T26102 T26101 cc and,GATA3
R19388 T26103 T26101 conj FOXP3,GATA3
R19389 T26104 T26098 advmod staining,analysis
R19390 T26105 T26106 auxpass is,shown
R19391 T26106 T26106 ROOT shown,shown
R19392 T26107 T26106 prep in,shown
R19393 T26108 T26109 compound panel,C
R19394 T26109 T26107 pobj C,in
R19395 T26110 T26106 prep following,shown
R19396 T26111 T26110 pobj exposure,following
R19397 T26112 T26111 prep of,exposure
R19398 T26113 T26115 compound CD4,CD45RA
R19399 T26114 T26115 compound +,CD45RA
R19400 T26115 T26112 pobj CD45RA,of
R19401 T26116 T26118 amod +,cells
R19402 T26117 T26118 compound T,cells
R19403 T26118 T26111 conj cells,exposure
R19404 T26119 T26120 aux to,anti-CD3
R19405 T26120 T26118 relcl anti-CD3,cells
R19406 T26121 T26120 dobj /,anti-CD3
R19407 T26122 T26121 nummod 28,/
R19408 T26123 T26121 punct ",",/
R19409 T26124 T26121 conj IL-4,/
R19410 T26125 T26124 cc and,IL-4
R19411 T26126 T26124 conj TGF-β,IL-4
R19412 T26127 T26106 punct .,shown
R19413 T26128 T26129 nsubj Data,are
R19414 T26129 T26129 ROOT are,are
R19415 T26130 T26129 attr representative,are
R19416 T26131 T26130 prep of,representative
R19417 T26132 T26134 nummod three,experiments
R19418 T26133 T26134 amod independent,experiments
R19419 T26134 T26131 pobj experiments,of
R19420 T26135 T26129 punct .,are
R19603 T26528 T26529 nummod GATA3,Acts
R19604 T26529 T26549 nsubjpass Acts,transduced
R19605 T26530 T26529 prep as,Acts
R19606 T26531 T26533 det a,Regulator
R19607 T26532 T26533 amod Negative,Regulator
R19608 T26533 T26530 pobj Regulator,as
R19609 T26534 T26533 prep of,Regulator
R19610 T26535 T26536 compound FOXP3,Expression
R19611 T26536 T26534 pobj Expression,of
R19612 T26537 T26536 punct (,Expression
R19613 T26538 T26536 appos A,Expression
R19614 T26539 T26536 punct ),Expression
R19615 T26540 T26547 amod Human,cells
R19616 T26541 T26547 amod naive,cells
R19617 T26542 T26547 nmod CD4,cells
R19618 T26543 T26547 nummod +,cells
R19619 T26544 T26547 nmod CDRA,cells
R19620 T26545 T26547 nummod +,cells
R19621 T26546 T26547 compound T,cells
R19622 T26547 T26549 nsubjpass cells,transduced
R19623 T26548 T26549 auxpass were,transduced
R19624 T26549 T26549 ROOT transduced,transduced
R19625 T26550 T26549 prep with,transduced
R19626 T26551 T26550 pobj 0,with
R19627 T26552 T26551 punct ",",0
R19628 T26553 T26551 conj 20,0
R19629 T26554 T26551 punct ",",0
R19630 T26555 T26551 conj 100,0
R19631 T26556 T26549 punct ",",transduced
R19632 T26557 T26549 cc and,transduced
R19633 T26558 T26559 nummod 500,nM
R19634 T26559 T26570 nsubjpass nM,analyzed
R19635 T26560 T26559 prep of,nM
R19636 T26561 T26562 nummod TAT-GATA3,protein
R19637 T26562 T26560 pobj protein,of
R19638 T26563 T26559 punct ",",nM
R19639 T26564 T26559 cc and,nM
R19640 T26565 T26566 amod intracellular,presence
R19641 T26566 T26559 conj presence,nM
R19642 T26567 T26559 prep of,nM
R19643 T26568 T26567 pobj GATA3,of
R19644 T26569 T26570 auxpass was,analyzed
R19645 T26570 T26549 conj analyzed,transduced
R19646 T26571 T26570 advcl using,analyzed
R19647 T26572 T26571 dobj FACS,using
R19648 T26573 T26571 prep following,using
R19649 T26574 T26577 amod anti-CD3,activation
R19650 T26575 T26577 nmod /,activation
R19651 T26576 T26577 nummod CD28,activation
R19652 T26577 T26573 dobj activation,following
R19653 T26578 T26577 prep of,activation
R19654 T26579 T26580 det the,cells
R19655 T26580 T26578 pobj cells,of
R19656 T26581 T26570 punct .,analyzed
R19657 T26582 T26583 amod GFP-positive,cells
R19658 T26583 T26584 nsubj cells,were
R19659 T26584 T26584 ROOT were,were
R19660 T26585 T26584 acomp gated,were
R19661 T26586 T26585 cc and,gated
R19662 T26587 T26585 conj analyzed,gated
R19663 T26588 T26587 prep for,analyzed
R19664 T26589 T26591 amod intracellular,expression
R19665 T26590 T26591 nummod FOXP3,expression
R19666 T26591 T26588 pobj expression,for
R19667 T26592 T26591 acl following,expression
R19668 T26593 T26596 det a,period
R19669 T26594 T26596 amod 2-d,period
R19670 T26595 T26596 amod incubation,period
R19671 T26596 T26592 dobj period,following
R19672 T26597 T26596 punct (,period
R19673 T26598 T26599 amod lower,panel
R19674 T26599 T26596 appos panel,period
R19675 T26600 T26596 punct ),period
R19676 T26601 T26584 punct .,were
R19677 T26602 T26603 nsubj Data,are
R19678 T26603 T26603 ROOT are,are
R19679 T26604 T26603 attr representative,are
R19680 T26605 T26604 prep of,representative
R19681 T26606 T26608 nummod four,experiments
R19682 T26607 T26608 amod independent,experiments
R19683 T26608 T26605 pobj experiments,of
R19684 T26609 T26603 punct .,are
R19685 T26610 T26616 punct (,−
R19686 T26611 T26616 dep B,−
R19687 T26612 T26611 punct ),B
R19688 T26613 T26615 compound CD4,CD25
R19689 T26614 T26615 compound +,CD25
R19690 T26615 T26616 nsubj CD25,−
R19691 T26616 T26618 amod −,cells
R19692 T26617 T26618 compound T,cells
R19693 T26618 T26620 nsubjpass cells,isolated
R19694 T26619 T26620 auxpass were,isolated
R19695 T26620 T26620 ROOT isolated,isolated
R19696 T26621 T26620 prep from,isolated
R19697 T26622 T26623 nummod D011,.10
R19698 T26623 T26626 nummod .10,mice
R19699 T26624 T26623 cc and,.10
R19700 T26625 T26626 amod D011.10xCD2-GATA3,mice
R19701 T26626 T26621 pobj mice,from
R19702 T26627 T26620 cc and,isolated
R19703 T26628 T26620 conj treated,isolated
R19704 T26629 T26628 prep with,treated
R19705 T26630 T26629 pobj OVA,with
R19706 T26631 T26630 cc and,OVA
R19707 T26632 T26630 conj TGF-β,OVA
R19708 T26633 T26628 prep for,treated
R19709 T26634 T26637 nummod 96,CD4
R19710 T26635 T26637 compound h.,CD4
R19711 T26636 T26637 compound Surface,CD4
R19712 T26637 T26633 pobj CD4,for
R19713 T26638 T26637 cc and,CD4
R19714 T26639 T26640 amod intracellular,FOXP3
R19715 T26640 T26637 conj FOXP3,CD4
R19716 T26641 T26642 auxpass were,measured
R19717 T26642 T26620 conj measured,isolated
R19718 T26643 T26642 agent by,measured
R19719 T26644 T26643 pobj FACS,by
R19720 T26645 T26620 punct .,isolated
R19721 T26646 T26647 det These,data
R19722 T26647 T26648 nsubj data,are
R19723 T26648 T26648 ROOT are,are
R19724 T26649 T26648 attr representative,are
R19725 T26650 T26649 prep of,representative
R19726 T26651 T26653 nummod three,experiments
R19727 T26652 T26653 amod independent,experiments
R19728 T26653 T26650 pobj experiments,of
R19729 T26654 T26648 punct .,are
R19730 T26655 T26656 punct (,C
R19731 T26656 T26667 meta C,harvested
R19732 T26657 T26656 punct ),C
R19733 T26658 T26659 det The,cells
R19734 T26659 T26667 nsubjpass cells,harvested
R19735 T26660 T26659 acl treated,cells
R19736 T26661 T26660 prep as,treated
R19737 T26662 T26661 prep in,as
R19738 T26663 T26664 punct (,B
R19739 T26664 T26662 pobj B,in
R19740 T26665 T26659 punct ),cells
R19741 T26666 T26667 auxpass were,harvested
R19742 T26667 T26667 ROOT harvested,harvested
R19743 T26668 T26667 cc and,harvested
R19744 T26669 T26671 nsubjpass mRNA,quantified
R19745 T26670 T26671 auxpass was,quantified
R19746 T26671 T26667 conj quantified,harvested
R19747 T26672 T26671 agent by,quantified
R19748 T26673 T26674 amod real-time,PCR
R19749 T26674 T26672 pobj PCR,by
R19750 T26675 T26671 prep for,quantified
R19751 T26676 T26675 pobj SMAD7,for
R19752 T26677 T26676 cc and,SMAD7
R19753 T26678 T26679 compound TGF-β,expression
R19754 T26679 T26676 conj expression,SMAD7
R19755 T26680 T26671 punct .,quantified
R19756 T26681 T26682 nsubj Bars,show
R19757 T26682 T26682 ROOT show,show
R19758 T26683 T26686 det the,SD
R19759 T26684 T26686 amod mean,SD
R19760 T26685 T26686 compound ±,SD
R19761 T26686 T26682 dobj SD,show
R19762 T26687 T26686 prep of,SD
R19763 T26688 T26690 nummod three,experiments
R19764 T26689 T26690 amod independent,experiments
R19765 T26690 T26687 pobj experiments,of
R19766 T26691 T26682 punct .,show
R20134 T27290 T27293 amod putative,region
R20135 T27291 T27293 nummod FOXP3,region
R20136 T27292 T27293 compound promoter,region
R20137 T27293 T27288 dobj region,containing
R20138 T27294 T27276 conj fused,transfected
R20139 T27295 T27294 prep to,fused
R20140 T27296 T27299 det the,gene
R20141 T27297 T27299 amod luciferase,gene
R20142 T27298 T27299 compound reporter,gene
R20143 T27299 T27295 pobj gene,to
R20144 T27300 T27276 punct .,transfected
R20145 T27301 T27302 nsubj Bars,show
R20146 T27302 T27302 ROOT show,show
R20147 T27303 T27306 det the,SD
R20148 T27304 T27306 amod mean,SD
R20149 T27305 T27306 compound ±,SD
R20150 T27306 T27302 dobj SD,show
R20151 T27307 T27306 prep of,SD
R20152 T27308 T27310 amod arbitrary,units
R20153 T27309 T27310 amod light,units
R20154 T27310 T27307 pobj units,of
R20155 T27311 T27310 acl normalized,units
R20156 T27312 T27311 prep for,normalized
R20157 T27313 T27314 compound renilla,luciferase
R20158 T27314 T27312 pobj luciferase,for
R20159 T27315 T27314 prep of,luciferase
R20160 T27316 T27318 nummod four,experiments
R20161 T27317 T27318 amod independent,experiments
R20162 T27318 T27315 pobj experiments,of
R20163 T27319 T27322 punct ;,measured
R20164 T27320 T27322 nsubjpass samples,measured
R20165 T27321 T27322 auxpass were,measured
R20166 T27322 T27302 ccomp measured,show
R20167 T27323 T27322 prep in,measured
R20168 T27324 T27323 pobj triplicates,in
R20169 T27325 T27302 punct .,show
R20170 T27326 T27327 punct (,B
R20171 T27327 T27327 ROOT B,B
R20172 T27328 T27327 punct ),B
R20173 T27329 T27332 compound Naive,cells
R20174 T27330 T27331 compound CD4,T
R20175 T27331 T27332 compound T,cells
R20176 T27332 T27334 nsubjpass cells,transfected
R20177 T27333 T27334 auxpass were,transfected
R20201 T27357 T27353 dobj SD,show
R20202 T27358 T27357 prep of,SD
R20203 T27359 T27361 nummod three,experiments
R20204 T27360 T27361 amod independent,experiments
R20205 T27361 T27358 pobj experiments,of
R20206 T27362 T27353 punct .,show
R20207 T27363 T27366 punct (,Naive
R20208 T27364 T27366 nmod C,Naive
R20209 T27365 T27366 punct ),Naive
R20210 T27366 T27381 nsubjpass Naive,transfected
R20211 T27367 T27368 punct (,left
R20212 T27368 T27369 amod left,panel
R20213 T27369 T27366 appos panel,Naive
R20214 T27370 T27369 punct ),panel
R20215 T27371 T27369 cc or,panel
R20216 T27372 T27369 conj memory,panel
R20217 T27373 T27375 punct (,panel
R20218 T27374 T27375 amod right,panel
R20219 T27375 T27369 appos panel,panel
R20220 T27376 T27369 punct ),panel
R20221 T27377 T27379 nummod CD4,cells
R20222 T27378 T27379 compound T,cells
R20223 T27379 T27381 nsubjpass cells,transfected
R20224 T27380 T27381 auxpass were,transfected
R20225 T27381 T27381 ROOT transfected,transfected
R20226 T27382 T27381 prep with,transfected
R20227 T27383 T27382 pobj wild-type,with
R20228 T27384 T27383 cc or,wild-type
R20229 T27385 T27386 det a,GATA3
R20230 T27386 T27387 nsubj GATA3,mutated
R20231 T27387 T27387 ROOT mutated,mutated
R20232 T27388 T27390 amod 511-FOXP3,reporter
R20233 T27389 T27390 compound promoter,reporter
R20234 T27390 T27391 compound reporter,construct
R20235 T27391 T27387 dobj construct,mutated
R20236 T27392 T27387 cc and,mutated
R20237 T27393 T27387 conj activated,mutated
R20238 T27394 T27393 prep with,activated
R20239 T27395 T27394 pobj PMA,with
R20240 T27396 T27395 cc and,PMA
R20241 T27397 T27395 conj ionomycin,PMA
R20242 T27398 T27387 punct .,mutated
R20243 T27399 T27400 nsubj Bars,show
R20244 T27400 T27400 ROOT show,show
R20245 T27401 T27404 det the,SD
R20246 T27402 T27404 amod mean,SD
R20247 T27403 T27404 compound ±,SD
R20248 T27404 T27400 dobj SD,show
R20249 T27405 T27404 prep of,SD
R20250 T27406 T27408 amod arbitrary,units
R20251 T27407 T27408 amod light,units
R20252 T27408 T27405 pobj units,of
R20253 T27409 T27408 acl normalized,units
R20254 T27410 T27409 prep for,normalized
R20255 T27411 T27412 compound renilla,luciferase
R20256 T27412 T27410 pobj luciferase,for
R20257 T27413 T27412 prep of,luciferase
R20258 T27414 T27416 nummod eight,experiments
R20259 T27415 T27416 amod independent,experiments
R20260 T27416 T27413 pobj experiments,of
R20261 T27417 T27420 punct ;,measured
R20262 T27418 T27420 nsubjpass samples,measured
R20263 T27419 T27420 auxpass were,measured
R20264 T27420 T27400 ccomp measured,show
R20265 T27421 T27420 prep in,measured
R20266 T27422 T27421 pobj triplicates,in
R20267 T27423 T27400 punct .,show
R20268 T27424 T27425 punct (,D
R20269 T27425 T27425 ROOT D,D
R20270 T27426 T27425 punct ),D
R20271 T27427 T27428 compound Nuclear,extracts
R20272 T27428 T27430 nsubjpass extracts,prepared
R20273 T27429 T27430 auxpass were,prepared
R20274 T27430 T27430 ROOT prepared,prepared
R20275 T27431 T27430 prep from,prepared
R20276 T27432 T27433 compound HEK,cells
R20277 T27433 T27431 pobj cells,from
R20278 T27434 T27430 conj transfected,prepared
R20279 T27435 T27434 prep with,transfected
R20280 T27436 T27451 nummod GATA3,cells
R20281 T27437 T27436 cc or,GATA3
R20282 T27438 T27440 det an,vector
R20283 T27439 T27440 amod empty,vector
R20284 T27440 T27436 conj vector,GATA3
R20285 T27441 T27436 punct ",",GATA3
R20286 T27442 T27445 punct (,Th1
R20287 T27443 T27445 nmod E,Th1
R20288 T27444 T27445 punct ),Th1
R20289 T27445 T27451 nmod Th1,cells
R20290 T27446 T27445 punct ",",Th1
R20291 T27447 T27445 conj Th2,Th1
R20292 T27448 T27447 punct ",",Th2
R20293 T27449 T27447 cc or,Th2
R20294 T27450 T27447 conj iTreg,Th2
R20295 T27451 T27435 pobj cells,with
R20296 T27452 T27451 cc and,cells
R20297 T27453 T27454 amod binding,factors
R20298 T27454 T27451 conj factors,cells
R20299 T27455 T27430 conj precipitated,prepared
R20300 T27456 T27455 xcomp using,precipitated
R20301 T27457 T27458 amod biotinylated,oligonucleotides
R20302 T27458 T27456 dobj oligonucleotides,using
R20303 T27459 T27430 punct .,prepared
R20304 T27460 T27461 det The,oligonucleotides
R20305 T27461 T27466 nsubjpass oligonucleotides,separated
R20306 T27462 T27463 compound transcription,factor
R20307 T27463 T27464 compound factor,complexes
R20308 T27464 T27466 nsubjpass complexes,separated
R20309 T27465 T27466 auxpass were,separated
R20310 T27466 T27466 ROOT separated,separated
R20311 T27467 T27466 prep on,separated
R20312 T27468 T27470 det a,gel
R20313 T27469 T27470 compound SDS-PAGE,gel
R20314 T27470 T27467 pobj gel,on
R20315 T27471 T27466 punct .,separated
R20316 T27472 T27473 det The,amounts
R20317 T27473 T27481 nsubjpass amounts,assessed
R20318 T27474 T27473 prep of,amounts
R20319 T27475 T27476 compound GATA3,protein
R20320 T27476 T27474 pobj protein,of
R20321 T27477 T27473 prep in,amounts
R20322 T27478 T27479 det the,precipitates
R20323 T27479 T27477 pobj precipitates,in
R20324 T27480 T27481 auxpass were,assessed
R20325 T27481 T27481 ROOT assessed,assessed
R20326 T27482 T27481 agent by,assessed
R20327 T27483 T27482 pcomp immunoblotting,by
R20328 T27484 T27483 prep with,immunoblotting
R20329 T27485 T27486 compound anti-GATA3,mAb
R20330 T27486 T27484 pobj mAb,with
R20331 T27487 T27481 punct .,assessed
R20332 T27488 T27490 amod Total,extracts
R20333 T27489 T27490 amod nuclear,extracts
R20334 T27490 T27493 nsubjpass extracts,run
R20335 T27491 T27493 auxpass were,run
R20336 T27492 T27493 advmod also,run
R20337 T27493 T27493 ROOT run,run
R20338 T27494 T27493 prep as,run
R20339 T27495 T27494 pobj controls,as
R20340 T27496 T27493 punct .,run
R20341 T27497 T27498 nsubj Data,are
R20342 T27498 T27498 ROOT are,are
R20343 T27499 T27498 attr representative,are
R20344 T27500 T27499 prep of,representative
R20345 T27501 T27503 nummod three,experiments
R20346 T27502 T27503 amod different,experiments
R20347 T27503 T27500 pobj experiments,of
R20348 T27504 T27498 punct .,are
R20349 T27505 T27513 punct (,analyzed
R20350 T27506 T27508 nmod F,iTreg
R20351 T27507 T27506 punct ),F
R20352 T27508 T27513 nsubjpass iTreg,analyzed
R20353 T27509 T27508 cc or,iTreg
R20354 T27510 T27511 amod Th2,cells
R20355 T27511 T27508 conj cells,iTreg
R20356 T27512 T27513 auxpass were,analyzed
R20357 T27513 T27513 ROOT analyzed,analyzed
R20358 T27514 T27513 agent by,analyzed
R20359 T27515 T27514 pobj ChIP,by
R20360 T27516 T27513 prep for,analyzed
R20361 T27517 T27516 pobj GATA3,for
R20362 T27518 T27513 oprd binding,analyzed
R20363 T27519 T27518 prep to,binding
R20364 T27520 T27522 det the,promoter
R20365 T27521 T27522 nummod FOXP3,promoter
R20386 T27542 T27543 nsubj Results,are
R20387 T27543 T27543 ROOT are,are
R20388 T27544 T27543 attr representative,are
R20389 T27545 T27544 prep of,representative
R20390 T27546 T27548 nummod three,experiments
R20391 T27547 T27548 amod independent,experiments
R20392 T27548 T27545 pobj experiments,of
R20393 T27549 T27543 punct .,are
R20131 T27287 T27280 conj vector,vector
R20132 T27288 T27287 acl containing,vector
R20133 T27289 T27293 det the,region
R20178 T27334 T27334 ROOT transfected,transfected
R20179 T27335 T27334 prep with,transfected
R20180 T27336 T27339 det the,reporter
R20181 T27337 T27339 nummod FOXP3,reporter
R20182 T27338 T27339 compound promoter,reporter
R20183 T27339 T27335 pobj reporter,with
R20184 T27340 T27334 advcl construct,transfected
R20185 T27341 T27340 advmod together,construct
R20186 T27342 T27340 prep with,construct
R20187 T27343 T27346 det a,vector
R20188 T27344 T27346 amod GATA3,vector
R20189 T27345 T27346 compound expression,vector
R20190 T27346 T27342 pobj vector,with
R20191 T27347 T27346 cc or,vector
R20192 T27348 T27350 det an,vector
R20193 T27349 T27350 amod empty,vector
R20194 T27350 T27346 conj vector,vector
R20195 T27351 T27334 punct .,transfected
R20196 T27352 T27353 nsubj Bars,show
R20197 T27353 T27353 ROOT show,show
R20198 T27354 T27357 det the,SD
R20199 T27355 T27357 amod mean,SD
R20200 T27356 T27357 compound ±,SD
R20100 T27256 T27257 nummod GATA3,Represses
R20101 T27257 T27265 nmod Represses,Jurkat
R20102 T27258 T27260 det the,FOXP3
R20103 T27259 T27260 compound Human,FOXP3
R20104 T27260 T27265 nmod FOXP3,Jurkat
R20105 T27261 T27265 nmod Promoter,Jurkat
R20106 T27262 T27263 punct (,A
R20107 T27263 T27261 ccomp A,Promoter
R20108 T27264 T27265 punct ),Jurkat
R20109 T27265 T27268 nmod Jurkat,cells
R20110 T27266 T27265 punct ",",Jurkat
R20111 T27267 T27268 compound Th2,cells
R20112 T27268 T27276 nsubjpass cells,transfected
R20113 T27269 T27268 punct ",",cells
R20114 T27270 T27268 cc and,cells
R20115 T27271 T27274 amod human,cells
R20116 T27272 T27274 amod primary,cells
R20117 T27273 T27274 compound CD4,cells
R20118 T27274 T27268 conj cells,cells
R20119 T27275 T27276 auxpass were,transfected
R20120 T27276 T27276 ROOT transfected,transfected
R20121 T27277 T27276 prep with,transfected
R20122 T27278 T27280 det an,vector
R20123 T27279 T27280 amod empty,vector
R20124 T27280 T27277 pobj vector,with
R20125 T27281 T27283 punct (,basic
R20126 T27282 T27283 compound pGL3,basic
R20127 T27283 T27280 appos basic,vector
R20128 T27284 T27283 punct ),basic
R20129 T27285 T27280 cc or,vector
R20130 T27286 T27287 det a,vector
R20366 T27522 T27519 pobj promoter,to
R20367 T27523 T27513 punct .,analyzed
R20368 T27524 T27526 dobj The,represents
R20369 T27525 T27526 nsubj input,represents
R20370 T27526 T27526 ROOT represents,represents
R20371 T27527 T27528 compound PCR,amplification
R20372 T27528 T27526 dobj amplification,represents
R20373 T27529 T27528 prep of,amplification
R20374 T27530 T27532 det the,sample
R20375 T27531 T27532 amod total,sample
R20376 T27532 T27529 pobj sample,of
R20377 T27533 T27532 punct ",",sample
R20378 T27534 T27537 nsubjpass which,subjected
R20379 T27535 T27537 auxpass was,subjected
R20380 T27536 T27537 neg not,subjected
R20381 T27537 T27532 relcl subjected,sample
R20382 T27538 T27537 prep to,subjected
R20383 T27539 T27540 det any,precipitation
R20384 T27540 T27538 pobj precipitation,to
R20385 T27541 T27526 punct .,represents
R322 T402 T403 auxpass were,shown
R323 T403 T378 conj shown,act
R324 T404 T405 aux to,be
R325 T405 T403 xcomp be,shown
R326 T406 T405 acomp critical,be
R327 T407 T406 prep for,critical
R328 T408 T407 pobj polarization,for
R329 T409 T408 prep between,polarization
R330 T410 T413 nummod Th1,phenotypes
R331 T411 T410 cc and,Th1
R332 T412 T410 conj Th2,Th1
R333 T413 T409 pobj phenotypes,between
R334 T414 T403 punct .,shown
R335 T415 T416 nsubj FOXP3,is
R336 T416 T443 ccomp is,are
R337 T417 T420 det an,factor
R338 T418 T420 amod essential,factor
R339 T419 T420 compound transcription,factor
R340 T420 T416 attr factor,is
R341 T421 T420 prep for,factor
R342 T422 T430 amod natural,Treg
R343 T423 T422 punct ",",natural
R344 T424 T422 conj thymus-derived,natural
R345 T425 T426 punct (,nTreg
R346 T426 T424 appos nTreg,thymus-derived
R347 T427 T426 punct ),nTreg
R348 T428 T424 cc and,thymus-derived
R349 T429 T424 conj inducible,thymus-derived
R350 T430 T421 pobj Treg,for
R351 T431 T432 punct (,iTreg
R352 T432 T430 appos iTreg,Treg
R353 T433 T430 punct ),Treg
R354 T434 T420 conj commitment,factor
R355 T435 T443 punct ;,are
R356 T436 T443 advmod however,are
R357 T437 T443 punct ",",are
R358 T438 T439 det the,mechanisms
R359 T439 T443 nsubj mechanisms,are
R360 T440 T439 acl regulating,mechanisms
R361 T441 T442 poss its,expression
R362 T442 T440 dobj expression,regulating
R363 T443 T443 ROOT are,are
R364 T444 T446 advmod as,unknown
R365 T445 T446 advmod yet,unknown
R366 T446 T443 acomp unknown,are
R367 T447 T443 punct .,are
R368 T448 T449 nsubj We,describe
R369 T449 T449 ROOT describe,describe
R370 T450 T451 det a,mechanism
R371 T451 T454 amod mechanism,polarization
R372 T452 T454 amod controlling,polarization
R373 T453 T454 compound iTreg,polarization
R374 T454 T449 dobj polarization,describe
R375 T455 T454 punct ",",polarization
R376 T456 T458 nsubjpass which,overruled
R377 T457 T458 auxpass is,overruled
R1410 T1670 T1673 nummod Th1,cells
R1411 T1671 T1670 cc or,Th1
R1412 T1672 T1670 conj Th2,Th1
R1413 T1673 T1669 pobj cells,as
R1414 T1674 T1673 punct ",",cells
R1415 T1675 T1676 nsubj which,are
R1416 T1676 T1673 relcl are,cells
R1417 T1677 T1678 advmod most,efficient
R1418 T1678 T1676 acomp efficient,are
R1419 T1679 T1676 prep in,are
R1420 T1680 T1679 pcomp defeating,in
R1421 T1681 T1684 amod microbial,invaders
R1422 T1682 T1681 cc or,microbial
R1423 T1683 T1681 conj parasitic,microbial
R1424 T1684 T1680 dobj invaders,defeating
R1425 T1685 T1680 advmod respectively,defeating
R1426 T1686 T1663 punct .,differentiate
R1427 T1687 T1688 det A,hallmark
R1428 T1688 T1695 nsubj hallmark,is
R1429 T1689 T1688 prep of,hallmark
R1430 T1690 T1694 nummod Th1,pathways
R1431 T1691 T1690 cc and,Th1
R1432 T1692 T1690 conj Th2,Th1
R1433 T1693 T1694 compound differentiation,pathways
R1434 T1694 T1689 pobj pathways,of
R1435 T1695 T1695 ROOT is,is
R1436 T1696 T1697 det the,exclusiveness
R1437 T1697 T1695 attr exclusiveness,is
R1438 T1698 T1697 prep of,exclusiveness
R1439 T1699 T1701 det the,phenotype
R1440 T1700 T1701 amod individual,phenotype
R1441 T1701 T1698 pobj phenotype,of
R1442 T1702 T1701 acl leading,phenotype
R1443 T1703 T1702 prep to,leading
R1444 T1704 T1705 det either,Th1
R1681 T1941 T1937 cc and,generated
R1682 T1942 T1943 auxpass are,assumed
R1683 T1943 T1937 conj assumed,generated
R1684 T1944 T1945 aux to,protect
R1685 T1945 T1943 xcomp protect,assumed
R1686 T1946 T1945 prep against,protect
R1687 T1947 T1948 det the,activity
R1688 T1948 T1946 pobj activity,against
R1689 T1949 T1948 prep of,activity
R1690 T1950 T1952 amod autoreactive,cells
R1691 T1951 T1952 compound T,cells
R1692 T1952 T1949 pobj cells,of
R1693 T1953 T1945 prep in,protect
R1694 T1954 T1955 det the,periphery
R1695 T1955 T1953 pobj periphery,in
R1696 T1956 T1937 punct .,generated
R1697 T1957 T1958 det These,cells
R1698 T1958 T1959 nsubj cells,express
R1699 T1959 T1959 ROOT express,express
R1700 T1960 T1963 det the,factor
R1701 T1961 T1963 compound forkhead,factor
R1702 T1962 T1963 compound transcription,factor
R1703 T1963 T1959 dobj factor,express
R1704 T1964 T1963 appos FOXP3,factor
R1705 T1965 T1964 cc and,FOXP3
R1706 T1966 T1967 advmod constitutively,express
R1707 T1967 T1959 conj express,express
R1708 T1968 T1967 dobj CD25,express
R1709 T1969 T1968 prep on,CD25
R1710 T1970 T1971 poss their,surface
R1711 T1971 T1969 pobj surface,on
R1712 T1972 T1967 punct ",",express
R1713 T1973 T1967 cc but,express
R1714 T1974 T1975 nsubj they,lack
R1715 T1975 T1967 conj lack,express
R1716 T1976 T1975 dobj expression,lack
R1717 T1977 T1976 prep of,expression
R4341 T5592 T5594 compound T,differentiation
R4342 T5593 T5594 compound cell,differentiation
R4343 T5594 T5595 nsubj differentiation,occurs
R4344 T5595 T5589 advcl occurs,occur
R4345 T5596 T5583 punct .,suggests
R4346 T5597 T5600 advmod Therefore,analyzed
R4347 T5598 T5600 punct ",",analyzed
R4348 T5599 T5600 nsubj we,analyzed
R4349 T5600 T5600 ROOT analyzed,analyzed
R4350 T5601 T5602 det the,expression
R4351 T5602 T5600 dobj expression,analyzed
R4352 T5603 T5602 prep of,expression
R4353 T5604 T5603 pobj FOXP3,of
R4354 T5605 T5604 cc and,FOXP3
R4355 T5606 T5604 conj GATA3,FOXP3
R4356 T5607 T5600 prep during,analyzed
R4357 T5608 T5609 det the,differentiation
R4358 T5609 T5607 pobj differentiation,during
R4359 T5610 T5609 prep of,differentiation
R4360 T5611 T5614 amod naive,cells
R4361 T5612 T5613 compound CD4,T
R4362 T5613 T5614 compound T,cells
R4363 T5614 T5610 pobj cells,of
R4364 T5615 T5609 prep into,differentiation
R4365 T5616 T5615 pobj Th0,into
R4366 T5617 T5626 punct (,IL-12
R4367 T5618 T5626 amod neutral,IL-12
R4368 T5619 T5626 punct ",",IL-12
R4369 T5620 T5626 nmod anti-IL-4,IL-12
R4370 T5621 T5620 punct ",",anti-IL-4
R4441 T5692 T5695 nsubj GATA3,showed
R4442 T5693 T5692 cc and,GATA3
R4443 T5694 T5692 conj FOXP3,GATA3
R4444 T5695 T5701 advcl showed,polarizes
R4445 T5696 T5697 amod similar,kinetics
R4446 T5697 T5695 dobj kinetics,showed
R4447 T5698 T5701 punct ",",polarizes
R4448 T5699 T5700 poss their,expression
R4601 T5852 T5851 advmod together,Taken
R5439 T7091 T7093 nsubjpass cells,activated
R5441 T7092 T7093 auxpass were,activated
R5442 T7093 T7093 ROOT activated,activated
R5628 T7325 T7326 punct (,mAbs
R5630 T7326 T7324 appos mAbs,antibodies
R5631 T7327 T7326 punct ),mAbs
R5633 T7328 T7324 punct ",",antibodies
R6777 T8858 T8862 nsubj IL-4,promote
R6778 T8859 T8862 aux did,promote
R6779 T8860 T8862 neg not,promote
R6780 T8861 T8862 advmod differentially,promote
R6851 T8932 T8926 punct ",",0
R6852 T8933 T8926 cc and,0
R6853 T8934 T8937 det the,cells
R6854 T8935 T8937 nummod FOXP3,cells
R6855 T8936 T8937 compound +,cells
R6856 T8937 T8940 nsubjpass cells,confined
R6857 T8938 T8940 auxpass were,confined
R6858 T8939 T8940 neg not,confined
R6859 T8940 T8896 conj confined,caused
R6860 T8941 T8940 prep to,confined
R6930 T9011 T9009 punct .,nonoverlapping
R6931 T9012 T9015 mark Since,known
R6932 T9013 T9015 nsubjpass FOXP3,known
R6933 T9014 T9015 auxpass is,known
R6934 T9015 T9030 advcl known,analyzed
R6935 T9016 T9017 aux to,act
R7805 T10333 T10334 nsubj GATA3,Is
R7806 T10334 T10353 ccomp Is,hypothesized
R7807 T10335 T10337 det a,Regulator
R7808 T10336 T10337 amod Negative,Regulator
R7809 T10337 T10334 attr Regulator,Is
R7810 T10338 T10337 prep of,Regulator
R7811 T10339 T10341 compound FOXP3,FOXP3
R7812 T10340 T10341 compound Expression,FOXP3
R7813 T10341 T10342 compound FOXP3,expression
R7814 T10342 T10338 pobj expression,of
R7815 T10343 T10346 amod decreased,expression
R7816 T10344 T10343 advmod once,decreased
R7817 T10345 T10346 amod GATA3,expression
R7818 T10346 T10347 nsubj expression,is
R7819 T10347 T10334 conj is,Is
R7820 T10348 T10347 acomp high,is
R7821 T10349 T10353 punct ;,hypothesized
R7822 T10350 T10353 advmod therefore,hypothesized
R7823 T10351 T10353 punct ",",hypothesized
R7824 T10352 T10353 nsubj we,hypothesized
R7825 T10353 T10353 ROOT hypothesized,hypothesized
R7864 T10392 T10389 appos ],expression
R7865 T10393 T10392 punct ",",]
R7866 T10394 T10392 cc and,]
R7867 T10395 T10396 advmod therefore,GATA3
R7868 T10396 T10397 nsubj GATA3,prevents
R7869 T10397 T10389 relcl prevents,expression
R7870 T10398 T10397 dobj differentiation,prevents
R7871 T10399 T10397 prep into,prevents
R7872 T10400 T10401 amod Th1,cells
R7873 T10401 T10399 pobj cells,into
R11508 T15252 T15255 nsubj cells,up-regulate
R11509 T15253 T15255 aux could,up-regulate
R11510 T15254 T15255 advmod efficiently,up-regulate
R11511 T15255 T15255 ROOT up-regulate,up-regulate
R11512 T15256 T15255 dobj FOXP3,up-regulate
R11513 T15257 T15258 advmod when,treated
R11514 T15258 T15255 advcl treated,up-regulate
R11515 T15259 T15258 prep with,treated
R11516 T15260 T15259 pobj TGF-β,with
R11517 T15261 T15262 aux to,induce
R11518 T15262 T15258 advcl induce,treated
R11519 T15263 T15264 amod iTreg,cells
R11520 T15264 T15262 dobj cells,induce
R11521 T15265 T15266 compound [,"10,28"
R11522 T15266 T15255 dep "10,28",up-regulate
R11523 T15267 T15268 nummod 34,]
R11524 T15268 T15266 appos ],"10,28"
R11525 T15269 T15255 punct ",",up-regulate
R11526 T15270 T15255 advcl suggesting,up-regulate
R11527 T15271 T15273 mark that,plays
R11528 T15272 T15273 nsubj FOXP3,plays
R11529 T15273 T15270 ccomp plays,suggesting
R11530 T15274 T15276 det an,role
R11531 T15275 T15276 amod important,role
R11532 T15276 T15273 dobj role,plays
R11533 T15277 T15276 prep in,role
R11534 T15278 T15281 det the,process
R11535 T15279 T15281 amod early,process
R11536 T15280 T15281 compound differentiation,process
R11537 T15281 T15277 pobj process,in
R11557 T15301 T15302 det This,commitment
R11558 T15302 T15304 nsubjpass commitment,characterized
R11559 T15303 T15304 auxpass is,characterized
R11560 T15304 T15304 ROOT characterized,characterized
R11561 T15305 T15304 agent by,characterized
R11562 T15306 T15307 amod competitive,expression
R11563 T15307 T15305 pobj expression,by
R11564 T15308 T15307 prep of,expression
R11565 T15309 T15310 det these,factors
R11566 T15310 T15308 pobj factors,of
R11567 T15311 T15313 compound [,]
R11568 T15312 T15313 compound "35,36",]
R11569 T15313 T15307 appos ],expression
R11570 T15314 T15307 punct ",",expression
R11571 T15315 T15318 dobj which,observed
R11572 T15316 T15318 nsubj we,observed
R11573 T15317 T15318 advmod also,observed
R11574 T15318 T15307 relcl observed,expression
R11575 T15319 T15318 prep in,observed
R11576 T15320 T15319 pcomp differentiating,in
R11577 T15321 T15324 nummod FOXP3,cells
R11578 T15322 T15324 nummod +,cells
R11579 T15323 T15324 compound iTreg,cells
R11580 T15324 T15320 dobj cells,differentiating
R11581 T15325 T15324 prep including,cells
R11582 T15326 T15327 det a,phase
R11583 T15327 T15325 pobj phase,including
R11584 T15328 T15327 prep of,phase
R11585 T15329 T15328 pobj co-expression,of
R11586 T15330 T15329 punct ",",co-expression
R11587 T15331 T15332 nsubj which,turns
R11588 T15332 T15327 relcl turns,phase
R11589 T15333 T15332 prep into,turns
R11590 T15334 T15335 amod nonoverlapping,expression
R11591 T15335 T15333 pobj expression,into
R11592 T15336 T15335 prep upon,expression
R11593 T15337 T15338 amod completed,differentiation
R11594 T15338 T15336 pobj differentiation,upon
R11595 T15339 T15304 punct .,characterized
R11596 T15340 T15345 prep In,appeared
R11597 T15341 T15343 det this,process
R11598 T15342 T15343 amod competitive,process
R11599 T15343 T15340 pobj process,In
R11600 T15344 T15345 nsubj TGF-β,appeared
R11601 T15345 T15345 ROOT appeared,appeared
R11602 T15346 T15347 aux to,be
R11603 T15347 T15345 xcomp be,appeared
R11604 T15348 T15347 acomp mandatory,be
R11605 T15349 T15347 prep for,be
R11606 T15350 T15351 det the,induction
R11607 T15351 T15349 pobj induction,for
R11608 T15352 T15351 prep of,induction
R11609 T15353 T15352 pobj FOXP3,of
R11610 T15354 T15347 punct ",",be
R11611 T15355 T15356 advmod possibly,by
R11612 T15356 T15347 prep by,be
R11613 T15357 T15356 pcomp keeping,by
R11614 T15358 T15359 det the,expression
R11615 T15359 T15357 dobj expression,keeping
R11616 T15360 T15359 prep of,expression
R11617 T15361 T15360 pobj GATA3,of
R11635 T15379 T15378 prep of,absence
R11636 T15380 T15381 compound polarization,factors
R11637 T15381 T15379 pobj factors,of
R11638 T15382 T15378 punct (,absence
R11639 T15383 T15378 appos Th0,absence
R11640 T15384 T15378 punct ),absence
R11641 T15385 T15386 amod such,as
R11642 T15386 T15372 prep as,differentiating
R11643 T15387 T15386 pobj IL-4,as
R11644 T15388 T15387 punct ",",IL-4
R11645 T15389 T15387 conj IL-12,IL-4
R11646 T15390 T15389 punct ",",IL-12
R11647 T15391 T15389 cc or,IL-12
R11648 T15392 T15393 nsubj TGF-β,showed
R11649 T15393 T15393 ROOT showed,showed
R11650 T15394 T15398 advmod only,expression
R11651 T15395 T15398 det a,expression
R11652 T15396 T15398 amod transient,expression
R11653 T15397 T15398 nummod FOXP3,expression
R11654 T15398 T15393 dobj expression,showed
R11655 T15399 T15393 cc and,showed
R11656 T15400 T15393 conj failed,showed
R11657 T15401 T15402 aux to,generate
R11658 T15402 T15400 xcomp generate,failed
R11659 T15403 T15404 det a,population
R11660 T15404 T15402 dobj population,generate
R11661 T15405 T15404 prep of,population
R11662 T15406 T15407 amod FOXP3-expressing,cells
R11663 T15407 T15405 pobj cells,of
R11664 T15408 T15400 punct ",",failed
R11665 T15409 T15393 cc but,showed
R11666 T15410 T15413 nsubj GATA3,were
R11667 T15411 T15410 cc and,GATA3
R11668 T15412 T15410 conj T-bet,GATA3
R11669 T15413 T15393 conj were,showed
R11670 T15414 T15413 acomp up-regulated,were
R11671 T15415 T15414 punct (,up-regulated
R11672 T15416 T15417 amod unpublished,data
R11673 T15417 T15414 appos data,up-regulated
R11674 T15418 T15414 punct ),up-regulated
R11675 T15419 T15413 punct .,were
R11676 T15420 T15452 advmod Interestingly,known
R11677 T15421 T15452 punct ",",known
R11678 T15422 T15427 mark as,described
R11679 T15423 T15427 nsubj we,described
R11680 T15424 T15423 cc and,we
R11681 T15425 T15423 conj others,we
R11682 T15426 T15427 advmod previously,described
R11683 T15427 T15452 advcl described,known
R11684 T15428 T15430 punct ",",factors
R11685 T15429 T15430 amod FOXP3-promoting,factors
R11686 T15430 T15427 dobj factors,described
R11687 T15431 T15430 punct ",",factors
R11688 T15432 T15433 amod such,as
R11689 T15433 T15430 prep as,factors
R11690 T15434 T15435 compound dexamethasone,[
R11691 T15435 T15437 nmod [,]
R11692 T15436 T15437 nummod 39,]
R11693 T15437 T15433 pobj ],as
R11694 T15438 T15437 punct ",",]
R11695 T15439 T15440 compound CTLA-4,[
R11696 T15440 T15452 nsubjpass [,known
R11713 T15457 T15455 pobj expression,of
R11714 T15458 T15454 conj [,inhibitors
R11715 T15459 T15458 nummod 42,[
R11716 T15460 T15461 nummod 45,]
R11717 T15461 T15458 appos ],[
R11718 T15462 T15452 punct .,known
R11719 T15463 T15467 advmod Therefore,induces
R11720 T15464 T15467 nsubj GATA3,induces
R11721 T15465 T15467 preconj not,induces
R11722 T15466 T15465 advmod only,not
R11723 T15467 T15467 ROOT induces,induces
R11724 T15468 T15467 dobj differentiation,induces
R11725 T15469 T15467 prep into,induces
R11726 T15470 T15471 amod Th2,cells
R11727 T15471 T15469 pobj cells,into
R11728 T15472 T15467 cc but,induces
R11729 T15473 T15474 advmod also,inhibits
R11730 T15474 T15467 conj inhibits,induces
R11731 T15475 T15476 nummod FOXP3,expression
R11732 T15476 T15474 dobj expression,inhibits
R11733 T15477 T15476 cc and,expression
R11734 T15478 T15476 conj commitment,expression
R11735 T15479 T15476 prep into,expression
R11736 T15480 T15481 compound iTreg,cells
R11737 T15481 T15479 pobj cells,into
R11738 T15482 T15467 punct .,induces
R11739 T15483 T15486 det The,IL-4
R11740 T15484 T15486 amod Th2,IL-4
R11741 T15485 T15486 compound cytokine,IL-4
R11742 T15486 T15494 nsubj IL-4,was
R11791 T15605 T15606 det a,distinction
R11793 T15606 T15604 pobj distinction,since
R11795 T15607 T15606 prep of,distinction
R11796 T15608 T15611 nmod nTreg,cells
R11797 T15609 T15608 cc and,nTreg
R11799 T15610 T15608 conj iTreg,nTreg
R11800 T15611 T15607 pobj cells,of
R11801 T15612 T15630 advcl is,exclude
R11802 T15613 T15612 advmod currently,is
R11804 T15614 T15612 neg not,is
R11920 T15567 T15570 amod natural,Treg
R11921 T15568 T15567 cc or,natural
R11922 T15569 T15567 conj inducible,natural
R11924 T15570 T15566 pobj Treg,of
R11928 T15571 T15573 punct (,+
R11931 T15572 T15573 compound CD25,+
R11935 T15573 T15562 appos +,decrease
R11939 T15574 T15573 cc and,+
R11943 T15575 T15576 compound Foxp3,+
R11948 T15576 T15573 conj +,+
R11951 T15577 T15573 punct ),+
R11954 T15578 T15559 dobj cells,resulted
R11956 T15579 T15580 nummod 7,d
R11959 T15580 T15578 prep d,cells
R11963 T15581 T15580 prep after,d
R11970 T15582 T15581 pobj treatment,after
R11974 T15583 T15580 punct .,d
R11978 T15584 T15585 poss Our,results
R11982 T15585 T15586 nsubj results,suggest
R11986 T15586 T15586 ROOT suggest,suggest
R11990 T15587 T15591 mark that,interfering
R11806 T15615 T15612 acomp possible,is
R11807 T15616 T15612 punct ",",is
R11809 T15617 T15622 mark because,express
R11810 T15618 T15619 compound iTreg,cells
R11811 T15619 T15622 nsubj cells,express
R11813 T15620 T15621 advmod also,transiently
R11814 T15621 T15622 advmod transiently,express
R11815 T15622 T15630 advcl express,exclude
R11816 T15623 T15622 dobj CD25,express
R11817 T15624 T15622 prep after,express
R11818 T15625 T15624 pobj activation,after
R11820 T15626 T15630 punct ",",exclude
R11821 T15627 T15630 nsubj we,exclude
R11822 T15628 T15630 aux can,exclude
R11824 T15629 T15630 neg not,exclude
R11825 T15630 T15630 ROOT exclude,exclude
R11826 T15631 T15635 mark that,inhibit
R11828 T15632 T15635 nsubj IL-4,inhibit
R11829 T15633 T15635 aux may,inhibit
R11831 T15634 T15635 advmod also,inhibit
R11832 T15635 T15630 ccomp inhibit,exclude
R11833 T15636 T15637 amod Foxp3,expression
R11834 T15637 T15635 dobj expression,inhibit
R11835 T15638 T15635 prep in,inhibit
R11837 T15639 T15640 compound nTreg,cells
R11838 T15640 T15638 pobj cells,in
R11839 T15641 T15640 prep in,cells
R11841 T15642 T15641 pobj vivo,in
R11842 T15643 T15635 cc or,inhibit
R11843 T15644 T15648 mark that,contribute
R11844 T15645 T15646 amod additional,effects
R11847 T15646 T15648 nsubj effects,contribute
R11848 T15647 T15648 aux may,contribute
R11850 T15648 T15635 conj contribute,inhibit
R11851 T15649 T15648 prep to,contribute
R11852 T15650 T15652 det the,drop
R11853 T15651 T15652 amod observed,drop
R11855 T15652 T15649 pobj drop,to
R11858 T15653 T15652 prep in,drop
R11859 T15654 T15655 amod Foxp3,expression
R11861 T15655 T15653 pobj expression,in
R11862 T15656 T15630 punct .,exclude
R11864 T15657 T15661 nsubjpass IL-4,shown
R11865 T15658 T15661 aux has,shown
R11866 T15659 T15661 advmod already,shown
R11867 T15660 T15661 auxpass been,shown
R11868 T15661 T15661 ROOT shown,shown
R11869 T15662 T15664 aux to,regulate
R11870 T15663 T15664 advmod negatively,regulate
R11871 T15664 T15661 xcomp regulate,shown
R11873 T15665 T15666 det the,development
R11874 T15666 T15664 dobj development,regulate
R11875 T15667 T15666 prep of,development
R11876 T15668 T15670 amod naive,cells
R11878 T15669 T15670 compound T,cells
R11879 T15670 T15667 pobj cells,of
R11880 T15671 T15664 prep into,regulate
R11881 T15672 T15671 pobj Th1,into
R11883 T15673 T15672 cc or,Th1
R11884 T15674 T15675 det the,IL-17
R11885 T15675 T15672 conj IL-17,Th1
R11911 T15694 T15693 punct ",",IL-6
R11912 T15695 T15701 nsubj which,inhibits
R11914 T15696 T15701 punct ",",inhibits
R11915 T15697 T15701 advcl combined,inhibits
R11916 T15698 T15697 prep with,combined
R11917 T15699 T15698 pobj TGF-β,with
R11918 T15700 T15701 punct ",",inhibits
R11919 T15701 T15693 relcl inhibits,IL-6
R11923 T15702 T15703 det the,generation
R11925 T15703 T15701 dobj generation,inhibits
R11926 T15704 T15703 prep of,generation
R11927 T15705 T15706 compound iTreg,cells
R11929 T15706 T15704 pobj cells,of
R11930 T15707 T15701 cc and,inhibits
R11932 T15708 T15701 conj induces,inhibits
R11933 T15709 T15708 dobj differentiation,induces
R11934 T15710 T15708 prep into,induces
R11936 T15711 T15713 det the,cells
R11937 T15712 T15713 compound Th17,cells
R11938 T15713 T15710 pobj cells,into
R11940 T15714 T15708 prep by,induces
R11941 T15715 T15717 det an,mechanism
R11942 T15716 T15717 amod unknown,mechanism
R11944 T15717 T15714 pobj mechanism,by
R11945 T15718 T15720 compound [,]
R11946 T15719 T15720 compound "48,49",]
R11947 T15720 T15717 appos ],mechanism
R11949 T15721 T15691 punct .,described
R11950 T15722 T15730 advmod Thus,regulated
R11952 T15723 T15724 det the,polarization
R11953 T15724 T15730 nsubjpass polarization,regulated
R12124 T15850 T15849 pobj GATA3,by
R12125 T15851 T15838 punct .,hypothesized
R12127 T15852 T15858 prep In,repressed
R12128 T15853 T15852 pobj fact,In
R12129 T15854 T15858 punct ",",repressed
R12131 T15855 T15857 amod GATA3-inducing,concentrations
R12132 T15856 T15857 compound IL-4,concentrations
R12133 T15857 T15858 nsubj concentrations,repressed
R12135 T15858 T15858 ROOT repressed,repressed
R12136 T15859 T15858 dobj TGF-β,repressed
R12138 T15860 T15858 conj mediated,repressed
R12139 T15861 T15862 nummod FOXP3,expression
R12140 T15862 T15860 dobj expression,mediated
R12142 T15863 T15860 punct ",",mediated
R12143 T15864 T15871 mark whereas,were
R12144 T15865 T15871 nsubj IL-4,were
R12146 T15866 T15868 advmod as,as
R12147 T15867 T15868 advmod well,as
R12150 T15868 T15865 cc as,IL-4
R12151 T15869 T15865 conj TGF-β,IL-4
R12153 T15870 T15865 conj signaling,IL-4
R12154 T15871 T15860 advcl were,mediated
R12155 T15872 T15871 acomp intact,were
R12156 T15873 T15858 punct .,repressed
R12158 T15874 T15875 det This,result
R12159 T15875 T15876 nsubj result,suggested
R12160 T15876 T15876 ROOT suggested,suggested
R12162 T15877 T15879 det a,mechanism
R12163 T15878 T15879 amod competitive,mechanism
R12165 T15879 T15876 dobj mechanism,suggested
R12166 T15880 T15879 prep between,mechanism
R12055 T15887 T15886 pcomp determining,in
R12060 T15888 T15889 compound lineage,commitment
R12062 T15889 T15887 dobj commitment,determining
R12065 T15890 T15887 prep during,determining
R12072 T15891 T15893 det the,phase
R12076 T15892 T15893 amod early,phase
R12080 T15893 T15890 pobj phase,during
R12084 T15894 T15893 prep of,phase
R12088 T15895 T15894 pobj differentiation,of
R12092 T15896 T15876 punct .,suggested
R12096 T15897 T15900 advmod Accordingly,investigated
R12100 T15898 T15900 punct ",",investigated
R12105 T15899 T15900 nsubj we,investigated
R12109 T15900 T15900 ROOT investigated,investigated
R12113 T15901 T15902 advmod naturally,high
R12120 T15902 T15904 amod high,cells
R12122 T15903 T15904 amod GATA3-expressing,cells
R12123 T15904 T15900 dobj cells,investigated
R12126 T15905 T15900 cc and,investigated
R12130 T15906 T15900 conj confirmed,investigated
R12134 T15907 T15908 det the,absence
R12137 T15908 T15906 dobj absence,confirmed
R12141 T15909 T15908 prep of,absence
R12145 T15910 T15909 pobj FOXP3,of
R12148 T15911 T15900 punct .,investigated
R12149 T15912 T15913 compound Protein,transduction
R12152 T15913 T15920 nsubj transduction,inhibited
R12157 T15914 T15913 prep of,transduction
R12161 T15915 T15914 pobj GATA3,of
R12164 T15916 T15913 prep into,transduction
R12167 T15881 T15880 pobj GATA3,between
R12180 T15983 T15982 prep with,exposure
R12181 T15984 T15983 pobj antigen,with
R12182 T15985 T15978 prep in,induce
R12184 T15986 T15987 det the,presence
R12185 T15987 T15985 pobj presence,in
R12186 T15988 T15987 prep of,presence
R12188 T15989 T15988 pobj TGF-β,of
R12189 T15990 T15976 punct .,failed
R12190 T15991 T16000 advmod Strikingly,have
R12192 T15992 T16000 punct ",",have
R12193 T15993 T16000 dep the,have
R12194 T15994 T16000 dep DO11,have
R12198 T15995 T15994 nummod .10,DO11
R12199 T15996 T16000 punct :,have
R12201 T15997 T15998 amod CD2-GATA3,mice
R12202 T15998 T16000 nsubj mice,have
R12204 T15999 T16000 aux do,have
R12205 T16000 T16000 ROOT have,have
R12206 T16001 T16004 amod peripheral,cells
R12208 T16002 T16004 nummod FOXP3,cells
R12209 T16003 T16004 compound +,cells
R12210 T16004 T16000 dobj cells,have
R12212 T16005 T16004 punct ",",cells
R12213 T16006 T16008 nsubj which,displayed
R12314 T15961 T15960 prep with,line
R12324 T15962 T15964 det the,overexpression
R12327 T15963 T15964 amod transient,overexpression
R12329 T15964 T15961 pobj overexpression,with
R12330 T15965 T15964 prep of,overexpression
R12334 T15966 T15965 pobj GATA3,of
R12337 T15967 T15964 prep in,overexpression
R12342 T15968 T15970 amod human,cells
R12448 T16198 T16199 det the,activity
R12453 T16199 T16197 dobj activity,increased
R12456 T16200 T16199 prep of,activity
R12457 T16201 T16203 det the,constructs
R12458 T16202 T16203 compound promoter,constructs
R12459 T16203 T16200 pobj constructs,of
R12460 T16204 T16197 punct ",",increased
R12462 T16205 T16206 advmod thus,revealing
R12466 T16206 T16197 advcl revealing,increased
R12473 T16207 T16209 det the,nature
R12478 T16208 T16209 amod repressive,nature
R12482 T16209 T16206 dobj nature,revealing
R12486 T16210 T16209 prep of,nature
R12490 T16211 T16213 det this,element
R12494 T16212 T16213 compound GATA,element
R12498 T16213 T16210 pobj element,of
R12502 T16214 T16213 prep in,element
R12506 T16215 T16217 compound memory,cells
R12510 T16216 T16217 compound T,cells
R12515 T16217 T16214 pobj cells,in
R12522 T16218 T16213 punct ",",element
R12527 T16219 T16221 nsubj which,express
R12531 T16220 T16221 advmod naturally,express
R12535 T16221 T16209 relcl express,nature
R12539 T16222 T16221 dobj GATA3,express
R12543 T16223 T16206 punct ",",revealing
R12547 T16224 T16228 mark whereas,seen
R12550 T16225 T16226 det no,difference
R12552 T16226 T16228 nsubjpass difference,seen
R12556 T16227 T16228 auxpass was,seen
R12559 T16228 T16206 advcl seen,revealing
R12563 T16229 T16228 prep in,seen
R12567 T16230 T16232 amod naive,cells
R12575 T16231 T16232 compound T,cells
R12610 T16242 T16243 compound GATA,element
R12614 T16243 T16245 nsubjpass element,bound
R12619 T16244 T16245 auxpass is,bound
R12621 T16245 T16245 ROOT bound,bound
R12624 T16246 T16245 agent by,bound
R12628 T16247 T16248 compound GATA3,protein
R12633 T16248 T16246 pobj protein,by
R12636 T16249 T16250 mark as,proven
R12641 T16250 T16245 advcl proven,bound
R12645 T16251 T16250 prep with,proven
R12649 T16252 T16253 amod pull-down,experiments
R12654 T16253 T16251 pobj experiments,with
R12658 T16254 T16245 punct .,bound
R12659 T16255 T16259 advmod Furthermore,shown
R12663 T16256 T16259 punct ",",shown
R12667 T16257 T16259 nsubjpass it,shown
R12674 T16258 T16259 auxpass is,shown
R12679 T16259 T16259 ROOT shown,shown
R12682 T16260 T16259 agent by,shown
R12687 T16261 T16260 pobj ChIP,by
R12691 T16262 T16264 nsubj that,binds
R12694 T16263 T16264 nsubj GATA3,binds
R12698 T16264 T16259 ccomp binds,shown
R12702 T16265 T16266 det this,element
R12706 T16266 T16264 dobj element,binds
R12710 T16267 T16264 advmod also,binds
R12714 T16268 T16264 prep in,binds
R12715 T16269 T16270 amod intact,cells
R12716 T16270 T16268 pobj cells,in
R12717 T16271 T16259 punct .,shown
R12718 T16272 T16274 nsubjpass It,known
R12719 T16273 T16274 auxpass is,known
R12720 T16274 T16304 ccomp known,acts
R12722 T16275 T16278 mark that,induce
R12724 T16276 T16278 nsubj GATA3,induce
R13524 T17892 T17891 punct .,Mice
R13525 T17893 T17895 amod Normal,mice
R13526 T17894 T17895 compound B6,mice
R13527 T17895 T17897 nsubjpass mice,purchased
R13528 T17896 T17897 auxpass were,purchased
R13529 T17897 T17897 ROOT purchased,purchased
R13530 T17898 T17897 prep from,purchased
R13531 T17899 T17901 det the,Laboratories
R13532 T17900 T17901 compound Jackson,Laboratories
R13533 T17901 T17898 pobj Laboratories,from
R13534 T17902 T17901 punct (,Laboratories
R13535 T17903 T17904 compound Bar,Harbor
R13536 T17904 T17901 appos Harbor,Laboratories
R13537 T17905 T17904 punct ",",Harbor
R13538 T17906 T17904 npadvmod Maine,Harbor
R13539 T17907 T17901 punct ),Laboratories
R13540 T17908 T17897 punct .,purchased
R13541 T17909 T17910 compound Transgenic,DO11
R13542 T17910 T17914 nsubj DO11,expressing
R13543 T17911 T17912 nummod .10,mice
R13544 T17912 T17910 appos mice,DO11
R13545 T17913 T17910 punct ",",DO11
R13546 T17914 T17930 advcl expressing,backcrossed
R13547 T17915 T17918 det a,receptor
R13548 T17916 T17918 compound T,receptor
R13549 T17917 T17918 compound cell,receptor
R13550 T17918 T17914 dobj receptor,expressing
R13551 T17919 T17918 prep for,receptor
R13552 T17920 T17919 pobj OVA323,for
R13553 T17921 T17922 nummod 339,peptide
R13554 T17922 T17918 appos peptide,receptor
R13555 T17923 T17922 prep in,peptide
R13556 T17924 T17925 det the,context
R13557 T17925 T17923 pobj context,in
R13558 T17926 T17925 prep of,context
R13559 T17927 T17926 pobj H-2d,of
R13560 T17928 T17930 punct ",",backcrossed
R13561 T17929 T17930 auxpass were,backcrossed
R13562 T17930 T17930 ROOT backcrossed,backcrossed
R13563 T17931 T17930 prep with,backcrossed
R13564 T17932 T17931 pobj mice,with
R13565 T17933 T17932 acl expressing,mice
R13566 T17934 T17947 nmod GATA-3,[
R13567 T17935 T17934 punct ",",GATA-3
R13568 T17936 T17934 acl driven,GATA-3
R13569 T17937 T17936 agent by,driven
R13570 T17938 T17943 det the,region
R13571 T17939 T17943 amod human,region
R13572 T17940 T17943 compound CD2,region
R13573 T17941 T17942 compound locus,control
R13574 T17942 T17943 compound control,region
R13575 T17943 T17937 pobj region,by
R13576 T17944 T17945 punct (,CD2-GATA3
R13577 T17945 T17943 appos CD2-GATA3,region
R13578 T17946 T17947 punct ),[
R13579 T17947 T17949 nmod [,]
R13580 T17948 T17949 nummod 22,]
R13581 T17949 T17933 dobj ],expressing
R13582 T17950 T17930 punct ",",backcrossed
R13583 T17951 T17930 advcl resulting,backcrossed
R13584 T17952 T17951 prep in,resulting
R13585 T17953 T17954 amod DO11.10xCD2-GATA3,mice
R13586 T17954 T17952 pobj mice,in
R13587 T17955 T17930 punct .,backcrossed
R13588 T17956 T17961 nsubjpass Mice,backcrossed
R13589 T17957 T17956 acl used,Mice
R13590 T17958 T17957 prep for,used
R13591 T17959 T17958 pobj experiments,for
R13592 T17960 T17961 auxpass were,backcrossed
R13593 T17961 T17961 ROOT backcrossed,backcrossed
R13594 T17962 T17961 prep on,backcrossed
R13595 T17963 T17965 det a,background
R13596 T17964 T17965 compound BALB/c,background
R13597 T17965 T17962 pobj background,on
R18580 T24864 T24865 det The,cells
R18581 T24865 T24867 nsubjpass cells,harvested
R18582 T24866 T24867 auxpass were,harvested
R18583 T24867 T24867 ROOT harvested,harvested
R18584 T24868 T24867 prep at,harvested
R18585 T24869 T24871 amod different,points
R18586 T24870 T24871 compound time,points
R18587 T24871 T24868 pobj points,at
R18588 T24872 T24867 punct ",",harvested
R18589 T24873 T24867 cc and,harvested
R18637 T24921 T24922 compound part,A
R18638 T24922 T24920 pobj A,in
R18639 T24923 T24922 prep of,A
R18640 T24924 T24925 det the,figure
R18641 T24925 T24923 pobj figure,of
R18642 T24926 T24906 punct .,shown
R18643 T24927 T24928 nsubj Data,are
R18644 T24928 T24928 ROOT are,are
R18645 T24929 T24928 attr representative,are
R18646 T24930 T24929 prep of,representative
R18647 T24931 T24933 nummod three,experiments

UBERON-AE

Id Subject Object Predicate Lexical cue
T57 444-450 http://purl.obolibrary.org/obo/UBERON_0002370 denotes thymus
T918 4592-4598 http://purl.obolibrary.org/obo/UBERON_0002370 denotes thymus
T919 4647-4653 http://purl.obolibrary.org/obo/UBERON_0002370 denotes thymus
T13431 32921-32927 http://purl.obolibrary.org/obo/UBERON_0002370 denotes thymus
T13432 33216-33222 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T18065 35841-35846 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T18776 37885-37890 http://purl.obolibrary.org/obo/UBERON_0002391 denotes lymph
T18777 37885-37896 http://purl.obolibrary.org/obo/UBERON_0000029 denotes lymph nodes
T18778 37901-37908 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleens
T18779 37994-38005 http://purl.obolibrary.org/obo/UBERON_0002371 denotes bone marrow
T19753 39398-39403 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T19754 39716-39721 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T22241 44457-44463 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleen
T22242 44468-44473 http://purl.obolibrary.org/obo/UBERON_0002391 denotes lymph
T22243 44468-44478 http://purl.obolibrary.org/obo/UBERON_0000029 denotes lymph node
T24097 10984-10991 http://purl.obolibrary.org/obo/UBERON_0012131 denotes central

GO-BP

Id Subject Object Predicate Lexical cue
T67 75-84 http://purl.obolibrary.org/obo/GO_0009058 denotes Formation
T68 159-172 http://purl.obolibrary.org/obo/GO_0006351 denotes Transcription
T69 410-423 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T70 957-963 http://purl.obolibrary.org/obo/GO_0040007 denotes growth
T71 1103-1118 http://purl.obolibrary.org/obo/GO_2000144 denotes transactivation
T72 1192-1203 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T73 1228-1243 http://purl.obolibrary.org/obo/GO_0006955 denotes immune response
T74 1235-1249 http://purl.obolibrary.org/obo/GO_0071353 denotes response. IL-4
T75 1235-1249 http://purl.obolibrary.org/obo/GO_0070670 denotes response. IL-4
T76 1418-1437 http://purl.obolibrary.org/obo/GO_0002507 denotes tolerance induction
T995 2859-2875 http://purl.obolibrary.org/obo/GO_0006955 denotes immune responses
T996 2950-2966 http://purl.obolibrary.org/obo/GO_0006955 denotes immune responses
T997 4093-4108 http://purl.obolibrary.org/obo/GO_0006955 denotes immune response
T998 2899-2914 http://purl.obolibrary.org/obo/GO_0001775 denotes cell activation
T999 2943-2966 http://purl.obolibrary.org/obo/GO_0045087 denotes innate immune responses
T1000 2943-2966 http://purl.obolibrary.org/obo/GO_0002227 denotes innate immune responses
T1001 2943-2966 http://purl.obolibrary.org/obo/GO_0045088 denotes innate immune responses
T1002 2943-2966 http://purl.obolibrary.org/obo/GO_0002218 denotes innate immune responses
T1003 2943-2966 http://purl.obolibrary.org/obo/GO_0045089 denotes innate immune responses
T1004 2943-2966 http://purl.obolibrary.org/obo/GO_0045824 denotes innate immune responses
T1005 3027-3046 http://purl.obolibrary.org/obo/GO_0030154 denotes cells differentiate
T1006 3905-3918 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T1007 4777-4790 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T1008 4020-4027 http://purl.obolibrary.org/obo/GO_0023052 denotes signals
T1009 5193-5196 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T1010 5882-5902 http://purl.obolibrary.org/obo/GO_0002360 denotes cell commitment as T
T2890 6431-6451 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T2891 6782-6788 http://purl.obolibrary.org/obo/GO_0007613 denotes memory
T2892 7037-7040 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T2893 8040-8049 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T2894 8877-8891 http://purl.obolibrary.org/obo/GO_0072602 denotes IL-4 secretion
T2895 9866-9880 http://purl.obolibrary.org/obo/GO_0072602 denotes IL-4 secretion
T2896 8882-8891 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T2897 9871-9880 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T2898 9225-9239 http://purl.obolibrary.org/obo/GO_0001775 denotes activated cell
T5217 11595-11615 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T5218 12540-12550 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T5219 12540-12566 http://purl.obolibrary.org/obo/GO_0009894 denotes regulation and degradation
T5220 12555-12566 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T6396 14695-14704 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T6397 14853-14862 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T6398 14718-14733 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T8273 17851-17857 http://purl.obolibrary.org/obo/GO_0040007 denotes growth
T8274 18102-18108 http://purl.obolibrary.org/obo/GO_0040007 denotes growth
T8275 18003-18016 http://purl.obolibrary.org/obo/GO_0051301 denotes cell division
T8276 18097-18108 http://purl.obolibrary.org/obo/GO_0016049 denotes cell growth
T8277 19969-19980 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T8278 20130-20139 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T8279 20173-20186 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T9855 20476-20486 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T9856 20901-20913 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T9857 21712-21715 http://purl.obolibrary.org/obo/GO_0007568 denotes age
T9858 22228-22248 http://purl.obolibrary.org/obo/GO_0036365 denotes activated with TGF-β
T9859 22228-22248 http://purl.obolibrary.org/obo/GO_0036366 denotes activated with TGF-β
T9860 22228-22248 http://purl.obolibrary.org/obo/GO_1901389 denotes activated with TGF-β
T9861 22228-22248 http://purl.obolibrary.org/obo/GO_1901390 denotes activated with TGF-β
T9862 22228-22248 http://purl.obolibrary.org/obo/GO_0036363 denotes activated with TGF-β
T9863 22228-22248 http://purl.obolibrary.org/obo/GO_0036364 denotes activated with TGF-β
T9864 22429-22438 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T9865 23401-23414 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T11586 23728-23741 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T11587 23888-23898 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T11588 24459-24478 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T11589 24692-24711 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T11590 24459-24478 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T11591 24692-24711 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T11592 24459-24478 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T11593 24692-24711 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T11594 24459-24478 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T11595 24692-24711 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T11596 24459-24478 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity
T11597 24692-24711 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity
T11598 24725-24731 http://purl.obolibrary.org/obo/GO_0007613 denotes memory
T13480 28036-28042 http://purl.obolibrary.org/obo/GO_0007613 denotes memory
T13481 33674-33680 http://purl.obolibrary.org/obo/GO_0007613 denotes memory
T13482 30514-30525 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T13483 32555-32566 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T13484 31638-31647 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T13485 31731-31744 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T13486 32948-32961 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T13487 34011-34024 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T13488 31941-31953 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T13489 34028-34048 http://purl.obolibrary.org/obo/GO_0006338 denotes chromatin remodeling
T13490 34157-34172 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T13491 34730-34750 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T13492 34903-34922 http://purl.obolibrary.org/obo/GO_0002507 denotes tolerance induction
T13493 35090-35109 http://purl.obolibrary.org/obo/GO_0002507 denotes tolerance induction
T13494 35079-35109 http://purl.obolibrary.org/obo/GO_0002660 denotes peripheral tolerance induction
T13495 35079-35109 http://purl.obolibrary.org/obo/GO_0002659 denotes peripheral tolerance induction
T13496 35079-35109 http://purl.obolibrary.org/obo/GO_0002465 denotes peripheral tolerance induction
T13497 35079-35109 http://purl.obolibrary.org/obo/GO_0002658 denotes peripheral tolerance induction
T17777 35621-35624 http://purl.obolibrary.org/obo/GO_0007568 denotes age
T18078 36084-36087 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T18079 36182-36196 http://purl.obolibrary.org/obo/GO_0072602 denotes IL-4 secretion
T18080 36187-36196 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T18337 36421-36424 http://purl.obolibrary.org/obo/GO_0033968 denotes GCA
T18338 36640-36643 http://purl.obolibrary.org/obo/GO_0033968 denotes GCA
T18339 36425-36428 http://purl.obolibrary.org/obo/GO_0004096 denotes CAT
T18340 37204-37207 http://purl.obolibrary.org/obo/GO_0004096 denotes CAT
T18341 37093-37096 http://purl.obolibrary.org/obo/GO_0004069 denotes AAT
T18342 37156-37159 http://purl.obolibrary.org/obo/GO_0047801 denotes CGT
T18791 38249-38264 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T18792 38281-38283 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T19112 38301-38321 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T19520 39274-39277 http://purl.obolibrary.org/obo/GO_0004096 denotes CAT
T19521 39330-39332 http://purl.obolibrary.org/obo/GO_0033968 denotes GA
T19765 39779-39798 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T19766 39779-39798 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T19767 39779-39798 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T19768 39779-39798 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T19769 39779-39798 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity
T20045 39996-40005 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T20046 39996-40010 http://purl.obolibrary.org/obo/GO_0032774 denotes synthesis. RNA
T20047 40101-40122 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse transcription
T20048 40166-40187 http://purl.obolibrary.org/obo/GO_0001171 denotes reverse-transcription
T20049 40109-40122 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T20050 40174-40187 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T20444 40757-40769 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T22259 44205-44208 http://purl.obolibrary.org/obo/GO_0007568 denotes Age
T22974 46649-46656 http://purl.obolibrary.org/obo/GO_0023052 denotes signals
T23814 10245-10265 http://purl.obolibrary.org/obo/GO_0030154 denotes differentiated cells
T24132 11287-11293 http://purl.obolibrary.org/obo/GO_0007613 denotes memory
T24705 13662-13686 http://purl.obolibrary.org/obo/GO_0030154 denotes cells to differentiating
T26346 22689-22712 http://purl.obolibrary.org/obo/GO_0001775 denotes activation of the cells
T26942 26557-26563 http://purl.obolibrary.org/obo/GO_0007613 denotes memory
T26943 27085-27098 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription

GO-MF

Id Subject Object Predicate Lexical cue
T631 720-725 http://purl.obolibrary.org/obo/GO_0005136 denotes 4 (IL
T632 1245-1249 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T633 1351-1355 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T634 1043-1050 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T2281 3508-3513 http://purl.obolibrary.org/obo/GO_0005136 denotes 4 (IL
T2282 3713-3717 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T2283 4177-4181 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T2284 6017-6021 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T2285 3719-3723 http://purl.obolibrary.org/obo/GO_0005137 denotes IL-5
T2286 3728-3733 http://purl.obolibrary.org/obo/GO_0005144 denotes IL-13
T2287 4170-4179 http://purl.obolibrary.org/obo/GO_0005138 denotes 6] and IL
T4454 8141-8149 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T5929 11753-11757 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T5930 11780-11785 http://purl.obolibrary.org/obo/GO_0005143 denotes IL-12
T7638 13785-13789 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7639 13831-13835 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7640 13956-13960 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7641 14146-14150 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7642 14176-14180 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7643 14369-14373 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7644 14441-14445 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7645 14559-14563 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7646 14602-14606 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7647 14788-14792 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7648 14831-14835 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7649 14937-14941 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T9216 17618-17622 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T9217 17668-17672 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T9218 17834-17838 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T9219 18061-18065 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T9220 18649-18653 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T9221 19296-19300 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T9222 19419-19423 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T9223 19676-19680 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T9224 20043-20047 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T10832 20789-20800 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA-binding
T10833 21208-21219 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA-binding
T10834 20789-20807 http://purl.obolibrary.org/obo/GO_0050692 denotes DNA-binding domain
T10835 21208-21226 http://purl.obolibrary.org/obo/GO_0050692 denotes DNA-binding domain
T10836 20793-20800 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T10837 21212-21219 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T10838 23382-23386 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T12586 23631-23638 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T12587 23679-23686 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T12588 24654-24661 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T12589 25233-25240 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T12590 25274-25281 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T12591 25462-25469 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T12592 25865-25872 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T12593 24459-24478 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T12594 24692-24711 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T12595 24459-24478 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T12596 24692-24711 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T12597 24459-24478 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T12598 24692-24711 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T12599 24459-24478 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T12600 24692-24711 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T12601 24459-24478 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity
T12602 24692-24711 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity
T12603 25696-25704 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T16685 29005-29009 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16686 29536-29540 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16687 29725-29729 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16688 29800-29804 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16689 29814-29818 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16690 29902-29906 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16691 29907-29911 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16692 30061-30065 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16693 30313-30317 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16694 30459-30463 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16695 30891-30895 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16696 30906-30910 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16697 31050-31054 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16698 31095-31099 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16699 31239-31243 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16700 31458-31462 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16701 31545-31549 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16702 31616-31620 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16703 34666-34670 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16704 29011-29016 http://purl.obolibrary.org/obo/GO_0005143 denotes IL-12
T16705 29549-29554 http://purl.obolibrary.org/obo/GO_0005144 denotes IL-13
T16706 31366-31371 http://purl.obolibrary.org/obo/GO_0005144 denotes IL-13
T16707 30559-30564 http://purl.obolibrary.org/obo/GO_0030367 denotes IL-17
T16708 30648-30652 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T16709 30900-30904 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T16710 33332-33339 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T16711 34388-34395 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T16712 34517-34524 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T16713 34803-34810 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T18256 36182-36186 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T18701 36421-36424 http://purl.obolibrary.org/obo/GO_0033968 denotes GCA
T18702 36640-36643 http://purl.obolibrary.org/obo/GO_0033968 denotes GCA
T18703 36425-36428 http://purl.obolibrary.org/obo/GO_0004096 denotes CAT
T18704 37204-37207 http://purl.obolibrary.org/obo/GO_0004096 denotes CAT
T18705 36977-36986 http://purl.obolibrary.org/obo/GO_0031386 denotes Ubiquitin
T18706 37568-37577 http://purl.obolibrary.org/obo/GO_0031386 denotes ubiquitin
T18707 37093-37096 http://purl.obolibrary.org/obo/GO_0004069 denotes AAT
T18708 37156-37159 http://purl.obolibrary.org/obo/GO_0047801 denotes CGT
T19010 38281-38283 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T19403 38562-38567 http://purl.obolibrary.org/obo/GO_0005143 denotes IL-12
T19404 38669-38674 http://purl.obolibrary.org/obo/GO_0005143 denotes IL-12
T19405 38762-38767 http://purl.obolibrary.org/obo/GO_0005143 denotes IL-12
T19406 38582-38586 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T19407 38630-38634 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T19408 38782-38786 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T19409 38843-38847 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T19711 39274-39277 http://purl.obolibrary.org/obo/GO_0004096 denotes CAT
T19712 39330-39332 http://purl.obolibrary.org/obo/GO_0033968 denotes GA
T20001 39779-39798 http://purl.obolibrary.org/obo/GO_0045289 denotes luciferase activity
T20002 39779-39798 http://purl.obolibrary.org/obo/GO_0047077 denotes luciferase activity
T20003 39779-39798 http://purl.obolibrary.org/obo/GO_0047712 denotes luciferase activity
T20004 39779-39798 http://purl.obolibrary.org/obo/GO_0050248 denotes luciferase activity
T20005 39779-39798 http://purl.obolibrary.org/obo/GO_0050397 denotes luciferase activity
T20878 41316-41320 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T20879 41424-41434 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T21593 41698-41706 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T21594 41823-41831 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T21595 42529-42537 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T21596 42604-42612 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T21597 43156-43166 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T21598 42613-42620 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T22157 43539-43546 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T22491 44185-44195 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T22492 44335-44339 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T22493 44409-44413 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T22494 44567-44571 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T22495 44636-44640 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T23717 7788-7792 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T24496 10632-10636 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T24497 10709-10713 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T24498 11261-11265 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T24499 11338-11342 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T24953 13378-13382 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T25637 16508-16512 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T25638 16636-16640 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T25639 17001-17005 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T25640 17177-17181 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T25641 17455-17459 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T26154 18665-18669 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T26155 18800-18804 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T26156 19204-19208 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T26157 19200-19206 http://purl.obolibrary.org/obo/GO_0032003 denotes 28, IL
T27574 26998-27005 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T27575 27413-27420 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T4442 7988-7992 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T4443 8115-8119 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T4444 8136-8140 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T4445 8877-8881 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T4446 8899-8903 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T4447 9100-9104 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T4448 9148-9152 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T4449 9256-9260 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T4450 9453-9457 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T4451 9528-9532 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T4452 9609-9613 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T4453 9866-9870 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7650 15079-15083 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7651 15319-15323 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7652 15343-15347 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7653 15663-15667 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7654 15937-15941 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7655 16067-16071 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7656 16180-16184 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7657 16273-16277 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7658 16460-16464 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T7659 15359-15369 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T7660 15561-15569 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody

GO-CC

Id Subject Object Predicate Lexical cue
T635 266-271 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T636 835-840 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T637 850-855 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T638 902-907 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T639 1282-1286 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2290 4053-4057 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2291 5178-5182 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2292 5573-5577 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2293 5685-5689 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2294 5882-5886 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2295 4720-4742 http://purl.obolibrary.org/obo/GO_0005938 denotes cells in the periphery
T2296 4720-4742 http://purl.obolibrary.org/obo/GO_0071944 denotes cells in the periphery
T2297 5193-5196 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T4459 6791-6796 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4460 6823-6828 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4461 7079-7084 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4462 7134-7139 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4463 7241-7246 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4464 7391-7396 http://purl.obolibrary.org/obo/GO_0005623 denotes Cells
T4465 8208-8213 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4466 8267-8272 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4467 8348-8353 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4468 8437-8442 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4469 8489-8494 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4470 8580-8585 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4471 8780-8785 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4472 8811-8816 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4473 8834-8839 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4474 8843-8848 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4475 9040-9045 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4476 9073-9078 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4477 9190-9195 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4478 9272-9277 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4479 9316-9321 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4480 9405-9410 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4481 9534-9539 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4482 9574-9579 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4483 9615-9620 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4484 9649-9654 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4485 9722-9727 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4486 9792-9797 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4487 9958-9963 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4488 10086-10091 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4489 10123-10128 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4490 7037-7040 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR
T4491 8141-8149 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T4492 8141-8149 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T5931 11501-11506 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5932 11723-11728 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5933 12242-12247 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5934 12283-12288 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5935 12346-12351 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5936 12392-12397 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5937 12657-12662 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5938 12845-12850 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5939 12956-12961 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5940 12990-12995 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5941 13068-13073 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5942 13146-13151 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7661 13871-13876 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7662 14035-14040 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7663 14062-14067 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7664 15247-15252 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7665 15523-15528 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7666 15745-15750 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7667 15873-15878 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7668 15915-15920 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7669 16124-16129 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7670 16157-16162 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7671 16170-16175 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7672 16266-16271 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7673 16415-16420 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7674 14951-14964 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T7675 15359-15369 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T7676 15561-15569 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T7677 15359-15369 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T7678 15561-15569 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T9225 17742-17747 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9226 17771-17776 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9227 17917-17922 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9228 17975-17980 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9229 18362-18367 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9230 18413-18418 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9231 18455-18460 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9232 18501-18506 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9233 18549-18554 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9234 18578-18583 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9235 18619-18624 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9236 19360-19365 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9237 19434-19439 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9238 19465-19470 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9239 19659-19664 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9240 19990-19995 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10839 20662-20667 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10840 20839-20844 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10841 20919-20924 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10842 21103-21108 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10843 21338-21343 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10844 21835-21840 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10845 21977-21982 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10846 22042-22047 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10847 22140-22145 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10848 22365-22370 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
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T21613 41957-41969 http://purl.obolibrary.org/obo/GO_0009986 denotes cell surface
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sentences

Id Subject Object Predicate Lexical cue
T58 0-106 Sentence denotes GATA3-Driven Th2 Responses Inhibit TGF-β1–Induced FOXP3 Expression and the Formation of Regulatory T Cells
T59 159-387 Sentence denotes Transcription factors act in concert to induce lineage commitment towards Th1, Th2, or T regulatory (Treg) cells, and their counter-regulatory mechanisms were shown to be critical for polarization between Th1 and Th2 phenotypes.
T60 388-575 Sentence denotes FOXP3 is an essential transcription factor for natural, thymus-derived (nTreg) and inducible Treg (iTreg) commitment; however, the mechanisms regulating its expression are as yet unknown.
T61 576-686 Sentence denotes We describe a mechanism controlling iTreg polarization, which is overruled by the Th2 differentiation pathway.
T62 687-782 Sentence denotes We demonstrated that interleukin 4 (IL-4) present at the time of T cell priming inhibits FOXP3.
T63 783-1004 Sentence denotes This inhibitory mechanism was also confirmed in Th2 cells and in T cells of transgenic mice overexpressing GATA-3 in T cells, which are shown to be deficient in transforming growth factor (TGF)-β–mediated FOXP3 induction.
T64 1005-1127 Sentence denotes This inhibition is mediated by direct binding of GATA3 to the FOXP3 promoter, which represses its transactivation process.
T65 1128-1244 Sentence denotes Therefore, this study provides a new understanding of tolerance development, controlled by a type 2 immune response.
T66 1245-1493 Sentence denotes IL-4 treatment in mice reduces iTreg cell frequency, highlighting that therapeutic approaches that target IL-4 or GATA3 might provide new preventive strategies facilitating tolerance induction particularly in Th2-mediated diseases, such as allergy.
T975 2849-2997 Sentence denotes Effective immune responses are characterized by T cell activation, which directs adaptive and innate immune responses to kill pathogens efficiently.
T976 2998-3181 Sentence denotes Dependent on the pathogen, T cells differentiate into different subtypes, such as Th1 or Th2 cells, which are most efficient in defeating microbial or parasitic invaders respectively.
T977 3182-3341 Sentence denotes A hallmark of Th1 and Th2 differentiation pathways is the exclusiveness of the individual phenotype leading to either Th1 or Th2, but not to mixed populations.
T978 3342-3482 Sentence denotes The exclusiveness of this mechanism is provided by a polarization process, where Th2 differentiation inhibits Th1 commitment and vice versa.
T979 3483-3619 Sentence denotes Specifically interleukin 4 (IL-4)–induced STAT6 and GATA3 inhibit differentiation into Th1 cells in the early phase of commitment [1,2].
T980 3620-3819 Sentence denotes GATA3 is sufficient to induce a Th2 phenotype [3] and acts not only through the induction of IL-4, IL-5 and IL-13, the Th2 cytokines, but also through the inhibition of Th1 cell-specific factors [3].
T981 3820-4074 Sentence denotes Recently, it was shown that T-bet directly modulates GATA3 function, suggesting that transcription factors compete in the early differentiation phase of T cells, potentially integrating environmental signals to finally imprint the T cell phenotype [4,5].
T982 4075-4250 Sentence denotes A GATA3-dominated immune response has been shown to be essential in airway hyperresonsiveness [6] and IL-4–dominated responses can break antigen-specific immune tolerance [7].
T983 4251-4430 Sentence denotes Overexpression of a dominant negative form of GATA3 [8] or treatment with antisense-mediated GATA3 blockade [9] decreased the severity of the allergic airway hyper-responsiveness.
T984 4431-4570 Sentence denotes The discovery of regulatory T (Treg) cells highlights another phenotype of T cells, which is essential for tolerance against self-antigens.
T985 4571-4743 Sentence denotes Naturally occurring, thymus-derived Treg (nTreg) cells are generated in the thymus and are assumed to protect against the activity of autoreactive T cells in the periphery.
T986 4744-4903 Sentence denotes These cells express the forkhead transcription factor FOXP3 and constitutively express CD25 on their surface, but they lack expression of Th1 or Th2 cytokines.
T987 4904-5047 Sentence denotes Particularly interesting are those Treg cells that are generated in the periphery and thus are potential targets for therapeutic interventions.
T988 5048-5117 Sentence denotes These induced Treg (iTreg) cells were reported to express FOXP3 [10].
T989 5118-5355 Sentence denotes The exact mechanisms of iTreg generation are unclear, but T cell receptor (TCR) triggering has been shown to induce FOXP3 expression and suppressive cells in human [11,12], however the phenotype appears to be of transient nature [13,14].
T990 5356-5625 Sentence denotes TGF-β has been demonstrated to be important for the persistent induction of these cells in vitro and in vivo, since animals lacking the TGF-βRII on T cells have fewer peripherally iTreg cells [15] and suffer from a T cell–dependent multiorgan inflammatory disease [16].
T991 5626-5791 Sentence denotes Although the effect of TGF-β on natural and inducible Treg cell induction has been demonstrated repeatedly [15,17], its molecular mechanisms remain to be identified.
T992 5792-5963 Sentence denotes The current study provides evidence that GATA3 and FOXP3 play a competitive role in iTreg cell commitment as T-bet and GATA3 for Th1 and Th2 differentiation, respectively.
T993 5964-6097 Sentence denotes We show that GATA3 inhibits FOXP3 induction and that IL-4 limits FOXP3 expression in a GATA3-mediated way, both in vitro and in vivo.
T994 6098-6273 Sentence denotes We also show that GATA3 directly binds to the FOXP3 promoter and thereby prevents the induction of this gene, demonstrating that Th2 differentiation overrules iTreg induction.
T2865 6284-6317 Sentence denotes FOXP3 Induction in T Cell Subsets
T2866 6318-6452 Sentence denotes It is assumed that FOXP3 expression can be induced in nonregulatory T cells, which is an important step in iTreg cell differentiation.
T2867 6453-6538 Sentence denotes However, it is not known if all CD4+ T cells have the same capacity to express FOXP3.
T2868 6539-6918 Sentence denotes To investigate whether FOXP3 can be expressed by any T cell subset or if expression is restricted to a distinct lineage, FOXP3 mRNA expression was analyzed in freshly isolated T cells such as CD25-depleted CD4+ cells, CD45RA+ naive or CD45RO+ memory T cells (Figure 1A), as well as T cells driven in vitro toward Th1, Th2, or iTreg phenotypes (Figure 1B; phenotype on Figure S1).
T2869 6919-7074 Sentence denotes The CD4+CD25–, CD45RA+, CD45RO+, and CD4+CD45RO+CD25– were able to significantly induce FOXP3 mRNA up to 30-fold upon TCR activation and addition of TGF-β.
T2870 7075-7116 Sentence denotes Th1 cells showed only a 10-fold increase.
T2871 7117-7211 Sentence denotes In contrast, Th2 cells stimulated under the same conditions did not increase FOXP3 expression.
T2872 7212-7376 Sentence denotes The in vitro generated iTreg cells were unable to further up-regulate FOXP3, which was already at high levels under the resting conditions (Figure 1B, right panel).
T2873 7387-8083 Sentence denotes Th2 Cells Cannot Induce FOXP3 Expression (A) Human T cells were activated with plate-bound anti-CD3/CD28 with or without TGF-β as indicated on the x-axis of (B). Cells were harvested after 5 days and FOXP3 mRNA was quantified by real-time PCR. Bars show the mean ± SD of 4 independent experiments. (B) In vitro differentiated Th1, Th2, or iTreg cells were activated with anti-CD3/CD28, TGF-β, or anti-IL-4 as indicated. The phenotype of these cells was confirmed by FACS and proliferation analysis (Figure S1). Bars show the mean ± SD of four independent experiments. Th2 cells are known to produce IL-4 upon activation, which may interact with TGF-β signaling and thus prevent FOXP3 induction.
T2874 8084-8233 Sentence denotes However, the neutralization of IL-4 with a blocking IL-4 antibody did not rescue FOXP3 expression in the differentiated Th2 cells (unpublished data).
T2875 8234-8326 Sentence denotes These data demonstrated that Th2 cells have a limited capacity to express FOXP3 (Figure 1B).
T2876 8327-8477 Sentence denotes The inability of Th2 cells to express FOXP3 was also documented at the single-cell level, confirming that Th2 cells lack FOXP3 expression (Figure 2A).
T2877 8478-8533 Sentence denotes Only iTreg cells expressed FOXP3 in resting conditions.
T2878 8534-8637 Sentence denotes Interestingly, we observed that resting iTreg cells express FOXP3 but show low CD25 surface expression.
T2879 8638-8786 Sentence denotes Repeated exposure to TGF-β did not further increase the FOXP3 expression in the iTreg lineage but transiently induced FOXP3 expression in Th1 cells.
T2880 8787-8980 Sentence denotes Naturally occurring Th2 cells such as CRTH2+ T cells, T cells isolated according to their IL-4 secretion, or an IL-4–producing T cell clone (BR8) were also lacking FOXP3 expression (Figure 2B).
T2881 8981-9091 Sentence denotes Furthermore TGF-β–mediated FOXP3 induction failed in these cells in contrast to the naive T cells (Figure 2B).
T2882 9092-9255 Sentence denotes Because IL-4 is the key Th2 cytokine, the expression of IL-4 and FOXP3 in freshly isolated CD4+ T cells was analyzed by fluorescence activated cell sorting (FACS).
T2883 9256-9378 Sentence denotes IL-4–expressing cells were most abundant among CD45RO+CD25– cells, which did not co-express FOXP3 (Figure 3A, left panel).
T2884 9379-9483 Sentence denotes In contrast, CD45RO+CD25+ cells abundantly expressed FOXP3, while lacking IL-4 (Figure 3A, right panel).
T2885 9484-9604 Sentence denotes As shown in Figure 3B, the frequency of the IL-4+ cells was always below 1% in the FOXP3+ cells close to the background.
T2886 9605-9740 Sentence denotes The IL-4+ cells were confined to the FOXP3– cells, as shown for the CD45RO+CD25–, CD45RO+CD25+, and CD45RO+CD25+high cells (Figure 3B).
T2887 9741-9840 Sentence denotes In addition, neither the Th2 clone (BR8) nor CRTH2 cells significantly expressed FOXP3 (Figure 3C).
T2888 9841-10013 Sentence denotes Cells enriched for their IL-4 secretion using the magnetic cell isolation technology contained some FOXP3-expressing cells, but importantly, the expression did not overlap.
T2889 10014-10129 Sentence denotes Taken together, these data indicate that FOXP3 was not expressed by Th2 cells and was not inducible in those cells.
T5207 11403-11452 Sentence denotes FOXP3 and GATA3 Kinetics in Differentiating Cells
T5208 11453-11623 Sentence denotes The limited capacity of differentiated effector cells to induce FOXP3 expression suggests that iTreg induction has to occur before effector T cell differentiation occurs.
T5209 11624-11814 Sentence denotes Therefore, we analyzed the expression of FOXP3 and GATA3 during the differentiation of naive CD4 T cells into Th0 (neutral, anti-IL-4, anti-IFN-γ, and anti IL-12), Th2, and iTreg phenotypes.
T5210 11815-12000 Sentence denotes After initiation of the differentiation process, FOXP3 and GATA3 showed a similar expression kinetic within the first 3 d, which are considered to be critical in T cell commitment [18].
T5211 12001-12111 Sentence denotes Under Th2 differentiation conditions, FOXP3 mRNA expression increased only marginally (Figure 4A, left panel).
T5212 12112-12322 Sentence denotes Thus, although GATA3 and FOXP3 showed similar kinetics, their expression polarizes at the end of the differentiation process when cells were cultured towards Th2 or iTreg cells, respectively (Figure 4A and 4B).
T5213 12323-12633 Sentence denotes Interestingly, the Th0 cells were expressing more FOXP3 than the Th2 cells, but expressed low levels of GATA3; however, the protein expression slightly differed from mRNA expression, suggesting also posttranslational regulation and degradation as potential additional mechanisms in the differentiation process.
T5214 12634-12798 Sentence denotes The phenotype of iTreg cells included an anergic phenotype upon anti-CD3 re-stimulation (Figure S1A), CD103, CTLA-4, GITR, and PD-1 surface expression (Figure S1B).
T5215 12799-13018 Sentence denotes On the single-cell level, it can be seen that cells progress through a transition phase, where GATA3 and FOXP3 expression coexist to some degree in the same cells, which is resolved in iTreg cells after 7 d (Figure 4B).
T5216 13019-13202 Sentence denotes Taken together, these data demonstrated that Th2 cells have lost their capacity to express FOXP3 and showed that Th2 and iTreg cells arise from two different differentiation pathways.
T6377 13785-13830 Sentence denotes IL-4 Inhibits TGF-β-Mediated iTreg Commitment
T6378 13831-13928 Sentence denotes IL-4 induces differentiation of naive T cells, upon antigen encounter, into the Th2 cell lineage.
T6379 13929-14041 Sentence denotes We therefore asked whether IL-4 is able to inhibit TGF-β induction of FOXP3 during the priming of naive T cells.
T6380 14042-14175 Sentence denotes Human CD4+CD45RA+ T cells were activated with plate-bound anti-CD3/CD28 in the presence of TGF-β and/or IL-4 and harvested after 5 d.
T6381 14176-14312 Sentence denotes IL-4 efficiently repressed the TGF-β–mediated induction of FOXP3 expression (Figure 5A) in a concentration-dependent manner (Figure 5B).
T6382 14313-14409 Sentence denotes Low levels of GATA3 were induced also in the absence of IL-4, as it was previously observed [3].
T6383 14410-14493 Sentence denotes However, at low concentration, IL-4 was able to marginally induce FOXP3 expression.
T6384 14494-14597 Sentence denotes Of note, GATA3 was also induced in the presence of TGF-β at high IL-4 concentration (Figure 5A and 5B).
T6385 14598-14914 Sentence denotes The IL-4–mediated prevention of FOXP3 expression was not caused by interferences of the receptor signaling, because the phosphorylation of SMAD2 or STAT6 was not affected by the addition of IL-4 and/or TGF-β, which demonstrates that IL-4 as well as TGF-β signaling were functional under these conditions (Figure 5C).
T6386 14915-15052 Sentence denotes Increasing amounts of IL-4 increase intracellular GATA3, whereas FOXP3 decreased, which is consistent with the mRNA analysis (Figure 5D).
T6387 15053-15162 Sentence denotes Furthermore, injection of IL-4 into wild-type B6 mice decreased the inducible or natural Treg number in vivo.
T6388 15163-15279 Sentence denotes A distinction of the Treg subsets is not possible, because recently activated iTreg cells also express surface CD25.
T6389 15280-15466 Sentence denotes We used complexes of recombinant mouse IL-4 (rmIL-4) plus anti-IL-4 monoclonal antibodies (mAbs), which have been shown to dramatically increase the potency of the cytokine in vivo [19].
T6390 15467-15622 Sentence denotes In these mice, the percentage of CD4+CD25+ and FOXP3+ T cells dramatically decreased when the antibody-cytokine immune complexes were injected (Figure S2).
T6391 15623-15921 Sentence denotes Upon administration of rmIL-4 plus anti-IL-4 mAb complexes, the total number of CD4+CD25+ T cell, as well as the Foxp3+ T cells diminished by half (Figure S2G and S2H), confirming that the lower percentage was not due to an increase in the CD4+CD25– cells, but a real decrease of CD4+CD25+ T cells.
T6392 15922-16042 Sentence denotes In conclusion, IL-4 negatively regulates the natural or inducible Treg cell turnover not only in vitro but also in vivo.
T6393 16043-16240 Sentence denotes To study the effects of IL-4 on already-existing human natural or inducible Treg cells, we exposed sorted CD25+ T cells (nTreg cells) to IL-4 and analyzed FOXP3 expression and suppressive capacity.
T6394 16241-16385 Sentence denotes In already-existing Treg cells, IL-4 failed to inhibit FOXP3 expression (Figure S3A), and the suppressive capacity was not altered (Figure S3C).
T6395 16386-16487 Sentence denotes Similarly pre-existing iTreg cells did not decrease FOXP3 expression upon IL-4 exposure (Figure S3B).
T8261 17545-17588 Sentence denotes The Role of TGF-β in T Cell Differentiation
T8262 17589-17816 Sentence denotes Although TGF-β–reduced CD25, IL-4 expression, and CD25 expression (Figure 6B), IL-4 significantly inhibited TGF-β–mediated induction of FOXP3 in naive T cells driven toward FOXP3+ T cells, as shown by FACS analysis (Figure 6A).
T8263 17817-17981 Sentence denotes It is known that IL-4 is a potent growth factor and may therefore favor the proliferation of FOXP3– cells and thus decrease the relative percentage of FOXP3+ cells.
T8264 17982-18139 Sentence denotes However, analysis of cell division kinetics by CFSE-labeling demonstrated that IL-4 did not differentially promote cell growth of FOXP3+ over that of FOXP3–.
T8265 18140-18217 Sentence denotes In fact both populations showed similarly enhanced proliferation (Figure 6A).
T8266 18218-18507 Sentence denotes Furthermore the TGF-β–mediated induction of FOXP3 expression was not caused by overgrowth of a CD25–FOXP3+ minority, since the number of FOXP3+ cells was low/absent in the purified CD4+CD45RA+ T cells (between 0% and 1%), and the FOXP3+ cells were not confined to the highly divided cells.
T8267 18508-18654 Sentence denotes CD25 was down-regulated in TGF-β–treated cells compare to activated T cells, which was even more pronounced in cells cultured with TGF-β and IL-4.
T8268 19280-19378 Sentence denotes The addition of IL-4 to iTreg-driving conditions decreased the number of FOXP3+ cells (Figure 6B).
T8269 19379-19502 Sentence denotes In line with the previous findings, the IL-4–producing cells and the FOXP3 expressing cells are nonoverlapping populations.
T8270 19503-19624 Sentence denotes Since FOXP3 is known to act as a repressor of cytokine expression [20], we therefore analyzed GATA3 and FOXP3 expression.
T8271 19625-20016 Sentence denotes The expression kinetic of naive T cells exposed to IL-4 and TGF-β demonstrated that GATA3 and FOXP3 are initially found in separate populations (day 2), but transiently co-express both factors (days 4–8), before establishing separate populations at the end of the differentiation process (day 10; Figure 6C), suggesting that GATA3 inhibits the development of iTreg cells by repressing FOXP3.
T8272 20017-20240 Sentence denotes These results showed that IL-4 acts in vitro as an inhibitor of FOXP3 expression, without interfering with TGF-β signaling, probably acting at the level of transcription factors, and possibly by a GATA3-dependent mechanism.
T9839 20242-20291 Sentence denotes GATA3 Is a Negative Regulator of FOXP3 Expression
T9840 20292-20424 Sentence denotes FOXP3 expression decreased once GATA3 expression is high; therefore, we hypothesized a potential role for GATA3 in repressing FOXP3.
T9841 20425-20668 Sentence denotes Besides GATA3′s well-known positive effect on gene regulation, GATA3′s repressive capabilities were previously shown to restrict Th1 commitment by inhibiting STAT4 expression [2,21], and therefore GATA3 prevents differentiation into Th1 cells.
T9842 20669-20894 Sentence denotes To investigate whether GATA3 can directly inhibit FOXP3 induction, we transduced GATA3 or a truncated GATA3 lacking the DNA-binding domain in human primary CD4+CD45RA+ T cells using a TAT-fused, recombinantly expressed GATA3.
T9843 20895-21003 Sentence denotes After transduction, the cells were activated with soluble anti-CD3/CD28 in the presence or absence of TGF-β.
T9844 21004-21134 Sentence denotes TAT-GATA3 was successfully transduced in a homogeneous and dose-dependent manner into human CD4+ T cells (Figure 7A, upper panel).
T9845 21135-21356 Sentence denotes TAT-GATA3 reduced FOXP3 expression in a dose-dependent manner, whereas a DNA-binding domain truncated version (TAT-ΔDBD-GATA3) did not affect FOXP3 expression as compared with expression in untransduced cells (Figure 7A).
T9846 21357-21556 Sentence denotes In addition, we analyzed the inhibitory effect of GATA3 on FOXP3 in transgenic DO11.10 mice, constitutively overexpressing GATA3 under the control of the CD2 locus control region (DO11.10xCD2-GATA3).
T9847 21557-21670 Sentence denotes The thymic selection into the CD4 lineage is largely intact in DO11.10xCD2-GATA3 (RW Hendriks, unpublished data).
T9848 21671-21773 Sentence denotes These mice develop lymphomas at an older age, but signs of autoimmune disease were not described [22].
T9849 21774-21956 Sentence denotes To investigate the effect of GATA3 on iTreg, CD4+CD62L+CD25– cells were isolated, activated with OVA in the presence or absence of TGF-β, and Foxp3 expression was analyzed after 4 d.
T9850 21957-22014 Sentence denotes The naive CD4+CD25– cells were Foxp3– (unpublished data).
T9851 22015-22126 Sentence denotes As described for the human cells, TGF-β dramatically up-regulated Foxp3 in the DO11.10 littermate control mice.
T9852 22127-22269 Sentence denotes In contrast, cells from the CD2-GATA3xDO11.10 mice showed dramatically reduced Foxp3 expression when activated with TGF-β and OVA (Figure 7B).
T9853 22270-22439 Sentence denotes All mice produced similar amounts of TGF-β; in addition, Smad7 was equally expressed [23] in T cells of both mice strains (Figure 7C), indicating intact TGF-β signaling.
T9854 23318-23455 Sentence denotes Taken together, these results demonstrated a repressive role of IL-4-induced GATA3 transcription factor in the generation of iTreg cells.
T11568 23457-23491 Sentence denotes GATA3 Represses the FOXP3 Promoter
T11569 23492-23669 Sentence denotes To investigate the molecular mechanism of GATA3-mediated repression of human FOXP3, the human FOXP3 promoter was studied and a palindromic binding site for GATA3 was discovered.
T11570 23670-23764 Sentence denotes The GATA-binding site is located 303 bp upstream from the transcription start site (TSS) [24].
T11571 23765-23899 Sentence denotes This site is highly conserved between humans, mice, and rats (Figure S4) and may therefore play an important role in FOXP3 regulation.
T11572 23900-23988 Sentence denotes The functional relevance of this site was studied using a FOXP3-promoter construct [24].
T11573 23989-24190 Sentence denotes We transfected human primary CD4+ T cells, in vitro differentiated Th2 cells, and Jurkat cells (Jurkat cells are known to constitutively express GATA3 [25,26]), and we measured FOXP3 promoter activity.
T11574 24191-24406 Sentence denotes The promoter was not active in the GATA3-expressing cell line Jurkat or in the in vitro-differentiated Th2 cells, whereas the construct was active in the CD4 cells, which express a lower amount of GATA3 (Figure 8A).
T11575 24407-24546 Sentence denotes Overexpression of GATA3 in naive T cells diminished luciferase activity of the FOXP3 promoter compared with the control vector (Figure 8B).
T11576 24547-24667 Sentence denotes To further address the function of the GATA3 site, we inserted a site-specific mutation deleting the GATA3-binding site.
T11577 24668-24901 Sentence denotes This mutation increased luciferase activity by 3-fold in memory CD4+CD45RO+ T cells, whereas no difference was observed in naive (GATA3–) CD4+CD45RA+ T cells, revealing a repressor activity of GATA3 on the FOXP3 promoter (Figure 8C).
T11578 24902-24992 Sentence denotes Furthermore GATA3 binds directly to the FOXP3 promoter as investigated by pull-down assay.
T11579 24993-25246 Sentence denotes HEK cells were transiently transfected with GATA3 or a control vector, and increasing amounts of lysates were incubated with oligonucleotides containing the GATA3 site of the FOXP3 promoter or a control oligonucleotide with a mutated GATA3-binding site.
T11580 25247-25311 Sentence denotes After the pull-down, GATA3 binding was detected by Western blot.
T11581 25312-25398 Sentence denotes Similarly, GATA3-expressing Th2 cells and iTreg cells were subjected to this approach.
T11582 25399-25498 Sentence denotes Only HEK cells overexpressing GATA3 and Th2 cells showed GATA3-binding activity (Figure 8D and 8E).
T11583 25499-25578 Sentence denotes These experiments demonstrated that GATA3 binds the palindromic FOXP3 promoter.
T11584 25579-25811 Sentence denotes To gain insights into the in vivo situation, we performed a chromatin immunoprecipitation (ChIP) using an anti-GATA3 antibody and showed that GATA3 binds to the FOXP3 promoter region in Th2 cells, but not in iTreg cells (Figure 7F).
T11585 25812-25926 Sentence denotes Taken together these data demonstrate that the GATA3-binding to the FOXP3 promoter is repressing FOXP3 expression.
T13433 27625-27796 Sentence denotes The current study reveals that FOXP3 induction, an important step in iTreg commitment, is inhibited by GATA3, which is the key regulator for polarization toward Th2 cells.
T13434 27797-27899 Sentence denotes After differentiation, the effector Th2 cells become refractory to conversion into a FOXP3+ phenotype.
T13435 27900-28003 Sentence denotes In accordance with other studies, we found that CD4+CD25– cells were able to up-regulate FOXP3 [12,27].
T13436 28004-28200 Sentence denotes Already-committed cells such as memory T cells and Th1 cells showed only moderate and transient FOXP3 induction, which is not sufficient to change the phenotype toward a regulatory T cell profile.
T13437 28201-28497 Sentence denotes In contrast, naive T cells could efficiently up-regulate FOXP3 when treated with TGF-β to induce iTreg cells [10,28–34], suggesting that FOXP3 plays an important role in the early differentiation process and may act in a way similar to that known for the Th1/Th2 decision factors T-bet and GATA3.
T13438 28498-28754 Sentence denotes This commitment is characterized by competitive expression of these factors [35,36], which we also observed in differentiating FOXP3+ iTreg cells including a phase of co-expression, which turns into nonoverlapping expression upon completed differentiation.
T13439 28755-28908 Sentence denotes In this competitive process TGF-β appeared to be mandatory for the induction of FOXP3, possibly by keeping the expression of GATA3 and T-bet low [37,38].
T13440 28909-29188 Sentence denotes In contrast, differentiating naive T cells in the absence of polarization factors (Th0) such as IL-4, IL-12, or TGF-β showed only a transient FOXP3 expression and failed to generate a population of FOXP3-expressing cells, but GATA3 and T-bet were up-regulated (unpublished data).
T13441 29189-29386 Sentence denotes Interestingly, as we and others previously described, FOXP3-promoting factors, such as dexamethasone [39], CTLA-4 [40], and estrogens [41], are also known as inhibitors of GATA3 expression [42–45].
T13442 29387-29518 Sentence denotes Therefore GATA3 not only induces differentiation into Th2 cells but also inhibits FOXP3 expression and commitment into iTreg cells.
T13443 29519-29690 Sentence denotes The Th2 cytokine IL-4 but not IL-13 (unpublished data) was able to inhibit TGF-β–mediated FOXP3 induction and therefore prevented conversion into the regulatory phenotype.
T13444 29691-29819 Sentence denotes To prove the inhibitory effect of IL-4 on inducible or natural Treg commitment in vivo, we treated mice with IL-4 and anti-IL-4.
T13445 29820-29892 Sentence denotes This has been shown to increase the effect of the cytokine in vivo [19].
T13446 29893-30035 Sentence denotes Only the IL-4/IL-4 mAb complex resulted in a decrease of the amount of natural or inducible Treg (CD25+ and Foxp3+) cells 7 d after treatment.
T13447 30036-30145 Sentence denotes Our results suggest that IL-4 is only interfering with the differentiation of naive T cells into iTreg cells.
T13448 30146-30458 Sentence denotes But since a distinction of nTreg and iTreg cells is currently not possible, because iTreg cells also transiently express CD25 after activation, we cannot exclude that IL-4 may also inhibit Foxp3 expression in nTreg cells in vivo or that additional effects may contribute to the observed drop in Foxp3 expression.
T13449 30459-30598 Sentence denotes IL-4 has already been shown to negatively regulate the development of naive T cells into Th1 or the IL-17–producing T cells (Th17) [46,47].
T13450 30599-30801 Sentence denotes Similar effects have been recently described for IL-6, which, combined with TGF-β, inhibits the generation of iTreg cells and induces differentiation into the Th17 cells by an unknown mechanism [48,49].
T13451 30802-30905 Sentence denotes Thus the polarization into iTreg cells is negatively regulated by the effector cytokines IL-4 and IL-6.
T13452 30906-31005 Sentence denotes IL-4 has been previously shown to induce the generation of FOXP3+ Treg cells out of CD4+CD25– [50].
T13453 31006-31156 Sentence denotes In those experiments, the concentrations of IL-4 used were low, and as we also observed, IL-4 at low concentration slightly enhanced FOXP3 expression.
T13454 31157-31238 Sentence denotes Importantly, these concentrations were not sufficient to induce GATA3 expression.
T13455 31239-31359 Sentence denotes IL-4 may favor proliferation of nTreg cells [47] or directly regulate FOXP3 expression in a STAT-dependent fashion [51].
T13456 31360-31520 Sentence denotes Since IL-13 does not effectively reduce FOXP3 and fails to induce GATA3, we hypothesized that the IL-4–dependent inhibition of FOXP3 could be mediated by GATA3.
T13457 31521-31660 Sentence denotes In fact, GATA3-inducing IL-4 concentrations repressed TGF-β–mediated FOXP3 expression, whereas IL-4 as well as TGF-β signaling were intact.
T13458 31661-31829 Sentence denotes This result suggested a competitive mechanism between GATA3 and FOXP3 transcription factors in determining lineage commitment during the early phase of differentiation.
T13459 31830-31932 Sentence denotes Accordingly, we investigated naturally high GATA3-expressing cells and confirmed the absence of FOXP3.
T13460 31933-32049 Sentence denotes Protein transduction of GATA3 into naive T cells inhibited FOXP3 induction in human, differentiating, naive T cells.
T13461 32050-32195 Sentence denotes This inhibitory effect of GATA3 was further confirmed in BALB/c transgenic mice, expressing GATA3 in T cells (DO11.10:CD2-GATA3 transgenic mice).
T13462 32196-32371 Sentence denotes In line with the transient overexpression of GATA3 in human T cells, cells of these mice failed to induce FOXP3 expression upon exposure with antigen in the presence of TGF-β.
T13463 32372-32529 Sentence denotes Strikingly, the DO11.10:CD2-GATA3 mice do have peripheral FOXP3+ cells, which however displayed a 10%–25% lower frequency compared to wild-type DO11.10 mice.
T13464 32530-32671 Sentence denotes Thus GATA3 restrains the development of certain Treg subsets, presumably the inducible, peripheral population and not those of thymic origin.
T13465 32672-32801 Sentence denotes Thymic T cells undergo a different maturation process, which may explain the insensitivity of nTreg to GATA3 overexpression [52].
T13466 32802-33172 Sentence denotes In contrast to the Th2-differentiating and iTreg-inhibiting function of GATA3 in peripheral T cells, GATA3 acts in the thymus together with other transcription factors such as the Repressor of GATA3 (ROG) in the differentiation process toward CD8 cells [53,54] or participates in complex transcriptional feedback network to regulate sympathoadrenal differentiation [55].
T13467 33173-33258 Sentence denotes Therefore, the role of GATA3 appears to be tissue specific and cannot be generalized.
T13468 33259-33369 Sentence denotes Our study demonstrates that GATA3 repressed FOXP3 expression directly by binding to the FOXP3 promoter region.
T13469 33370-33525 Sentence denotes A palindromic GATA-site is located 303 bp upstream of the TSS in a highly conserved region, which we have previously identified as the FOXP3 promoter [24].
T13470 33526-33797 Sentence denotes Site-specific mutation of this site increased the activity of the promoter constructs, thus revealing the repressive nature of this GATA element in memory T cells, which naturally express GATA3, whereas no difference was seen in naive T cells, which do not express GATA3.
T13471 33798-33891 Sentence denotes This palindromic GATA element is bound by GATA3 protein as proven with pull-down experiments.
T13472 33892-33976 Sentence denotes Furthermore, it is shown by ChIP that GATA3 binds this element also in intact cells.
T13473 33977-34184 Sentence denotes It is known that GATA3 can induce transcription by chromatin remodeling [56], by directly transactivating promoters [36], or, as shown in the current study, acts as a repressor of gene expression [21,57–59].
T13474 34185-34291 Sentence denotes Therefore keeping GATA3 expression low might be required to induce efficient FOXP3+ iTreg cell generation.
T13475 34292-34581 Sentence denotes The molecular interactions enabling GATA3 to inhibit FOXP3 are not identified yet, but the GATA-binding site is located adjacent to positive, inducing sites, composed of AP-1-NFATc2 sites [24], and GATA3 may compete with the binding of AP-1/NFAT to the promoter (unpublished observations).
T13476 34582-34671 Sentence denotes In summary, we demonstrated that FOXP3 is negatively regulated by cytokines such as IL-4.
T13477 34672-34845 Sentence denotes GATA3 acts as an inhibitor of FOXP3 expression in early T cell differentiation, as well as in differentiated Th2 cells by directly binding and repressing the FOXP3 promoter.
T13478 34846-34954 Sentence denotes We therefore describe a new mechanism of how Il-4 avoids tolerance induction by repressing FOXP3 expression.
T13479 34955-35146 Sentence denotes These findings will give new perspectives toward understanding molecular mechanisms of iTreg induction and thus pathways of peripheral tolerance induction, particularly in allergy and asthma.
T17772 35171-35176 Sentence denotes Mice.
T17773 35177-35257 Sentence denotes Normal B6 mice were purchased from the Jackson Laboratories (Bar Harbor, Maine).
T17774 35258-35504 Sentence denotes Transgenic DO11.10 mice, expressing a T cell receptor for OVA323–339 peptide in the context of H-2d, were backcrossed with mice expressing GATA-3, driven by the human CD2 locus control region (CD2-GATA3) [22], resulting in DO11.10xCD2-GATA3 mice.
T17775 35505-35636 Sentence denotes Mice used for experiments were backcrossed on a BALB/c background for a minimum of eight generations and used at an age of 8–12 wk.
T17776 35637-35780 Sentence denotes Mice were housed under specific pathogen-free conditions and all animal studies were performed according to institutional and state guidelines.
T18073 35782-35808 Sentence denotes Isolation of CD4+ T cells.
T18074 35809-35964 Sentence denotes CD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60].
T18075 35965-36035 Sentence denotes The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%.
T18076 36036-36143 Sentence denotes Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive.
T18077 36144-36197 Sentence denotes The clones were characterized by high IL-4 secretion.
T18328 36199-36226 Sentence denotes Quantitative real-time PCR.
T18329 36227-36655 Sentence denotes The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′.
T18330 36656-36845 Sentence denotes The prepared cDNAs were amplified using SYBR-PCR mastermix (Biorad) according to the recommendations of the manufacturer in an ABI PRISM 7000 Sequence Detection System (Applied Biosystems).
T18331 36846-36976 Sentence denotes Quantitative PCR of murine samples was performed with Brilliant SYBR Green QPCR master mix (Stratagene) and the following primers:
T18332 36977-37223 Sentence denotes Ubiquitin C, 5′- AGG TCA AAC AGG AAG ACA GAC GTA-3′ and 5′-TCACACCCAAGAACAAGCACA-3′; Smad-7, 5′-GAA ACC GGG GGA ACG AAT TAT-3′ and 5′-CGC GAG TCT TCT CCT CCC A-3′; TGF-ß1, 5′-TGA CGT CAC TGG AGT TGT ACG G-3′ and 5′-GGT TCA TGT CAT GGA TGG TGC-3′.
T18333 37224-37362 Sentence denotes Primer pairs were evaluated for integrity by analysis of the amplification plot, dissociation curves, and efficiency of PCR amplification.
T18334 37363-37515 Sentence denotes PCR conditions were 10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C and 60 °C for 1 min using an 7300 real-time PCR system (Applied Biosystems).
T18335 37516-37665 Sentence denotes PCR amplification of the housekeeping gene encoding ubiquitin C was performed during each run for each sample to allow normalization between samples.
T18336 37666-37781 Sentence denotes Relative quantification and calculation of the range of confidence was performed using the comparative ΔΔCT method.
T18787 37783-37813 Sentence denotes Inducible murine Treg culture.
T18788 37814-37945 Sentence denotes Naive CD4+ T cells (CD4+, CD62L+, and CD25–) were isolated from pooled lymph nodes and spleens by FACS (FACS Aria, BD Biosciences).
T18789 37946-38157 Sentence denotes 5 × 105 T cells were co-cultured with 2.5 × 104 bone marrow–derived dendritic cells [62] and 0.01 μg/ml OVA323–339 peptide (Ansynth) in the presence or absence of 20-ng/μl rhTGF-ß1 (Peprotech) in 48-well plates.
T18790 38158-38288 Sentence denotes After 4 d, cells were harvested and analyzed for intracellular FOXP3 expression by FACS or gene expression by quantitative RT-PCR.
T19106 38290-38322 Sentence denotes In vitro T cell differentiation.
T19107 38323-38552 Sentence denotes CD4+ CD45RA+ magnetically-sorted (CD45RO depletion, MACS, according to the protocol of the manufacturer) cells were stimulated with immobilized plate-bound anti-CD3 (1 μg/ml, Okt3, IgG1) and anti-CD28 (2 μg/ml) in Th1 conditions:
T19108 38553-38620 Sentence denotes 25 ng/ml IL-12, 5 μg/ml anti-IL-4 (R&D systems); in Th2 conditions:
T19109 38621-38712 Sentence denotes 25 ng/ml IL-4, 5 μg/ml anti-IFN-γ, 5 μg/ml anti-IL-12 (R&D systems); or in Treg conditions:
T19110 38713-38787 Sentence denotes 10 ng/ml TGF-β, 5 μg/ml anti-IFN-γ, 5 μg/ml anti-IL-12, 5 μg/ml anti-IL-4.
T19111 38788-38859 Sentence denotes Proliferating cells were expanded in medium containing IL-2 (30 ng/ml).
T19516 38861-38929 Sentence denotes Cloning of the FOXP3 promoter and construction of mutant constructs.
T19517 38930-39047 Sentence denotes The FOXP3 promoter was cloned into the pGL3 basic vector (Promega Biotech) to generate the pGL3 FOXP3 −511/+176 [24].
T19518 39048-39204 Sentence denotes Site-directed mutagenesis in the FOXP3 promoter region were introduced using the QuickChange kit (Stratagene), according to the manufacturer's instructions.
T19519 39205-39333 Sentence denotes The following primer and its complementary strand were used: GTT TCT CAT GAG CCC TAT TAA GTC ATT CTT ACC TCT CAC CTC TGT GGT GA.
T19761 39335-39374 Sentence denotes Transfections and reporter gene assays.
T19762 39375-39452 Sentence denotes T cells were rested in serum-free AIM-V medium (Life Technologies) overnight.
T19763 39453-39911 Sentence denotes 3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions.
T19764 39912-39971 Sentence denotes Data were normalized by the activity of renilla luciferase.
T20042 39973-40006 Sentence denotes RNA isolation and cDNA synthesis.
T20043 40007-40100 Sentence denotes RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol.
T20044 40101-40280 Sentence denotes Reverse transcription of human samples was performed with TaqMan reverse-transcription reagents (Applied Biosystems) with random hexamers according to the manufacturer's protocol.
T20171 40282-40307 Sentence denotes Recombinant TAT proteins.
T20172 40308-40499 Sentence denotes The cDNAs encoding GATA3 protein or the truncated GATA3 (lacking the two zinc fingers) were cloned in frame into an expression vector along with the TAT sequence as previously described [63].
T20173 40500-40625 Sentence denotes Proteins were expressed in BL21 Star (DE3)pLysS (Invitrogen) and lysates were purified by Ni2+-chelate column chromatography.
T20174 40626-40745 Sentence denotes Both TAT-linked proteins were more than 95% pure, based on Coomassie blue staining of sodium disulfate acrylamide gels.
T20439 40747-40770 Sentence denotes TAT-GATA3 transduction.
T20440 40771-40919 Sentence denotes CD4+CD45RA+ cells were cultured in AIMV medium and transduced with 20 nM, 10 nM, or 500 nM of full-length or truncated GATA3 over the course of 4 h.
T20441 40920-41024 Sentence denotes After 4 h, the cells were washed and activated with soluble anti-CD3 and anti-CD28 and TGF-β (10 ng/ml).
T20442 41025-41085 Sentence denotes Each day, the TAT proteins were freshly added to the medium.
T20443 41086-41152 Sentence denotes FOXP3 expression was measured after 5 d by intracellular staining.
T20710 41154-41186 Sentence denotes Intracellular cytokine staining.
T20711 41187-41282 Sentence denotes T cells were stimulated with 2 × 10−7 M PMA and 1 μg/ml of ionomycin (Sigma Chemicals) for 4 h.
T20712 41283-41336 Sentence denotes The following mAb was used: anti-IL-4-PE (8D4–8, BD).
T20713 41337-41435 Sentence denotes Matched isotype controls were used at the same protein concentration as the respective antibodies.
T20714 41436-41576 Sentence denotes Four-color FACS was performed using an EPICS XL-MCL (Beckman Coulter) using the software Expo32 version for data acquisition and evaluation.
T20958 41578-41593 Sentence denotes Flow cytometry.
T20959 41594-41952 Sentence denotes For analysis of FOXP3 expression at the single-cell level, cells were first stained with the monoclonal antibody CD25 (Beckman Coulter), and after fixation and permeabilization, cells were incubated with PE-conjugated monoclonal antibody PCH101 (anti-human FOXP3; eBioscience) based on the manufacturer's recommendations and subjected to FACS (EPICS XL-MCL).
T20960 41953-42278 Sentence denotes For cell surface marker staining, cells were incubated for 20 min at 4°C in staining buffer with the following antibodies: anti–CD152-PE (CTLA-4; BD), anti–PD-1 (eBiosciences), anti-GITR (R & D Systems), anti-CD69 (Beckman Coulter), anti-CD103 (DakoCytomation), anti-CD62L (Beckman Coulter), or anti-HLA-DR (Beckman Coulter).
T20961 42279-42349 Sentence denotes The controls were FITC, PE, or ECD-conjugated mouse IgG1 or rat IgG2a.
T20962 42350-42512 Sentence denotes For staining of mouse cells, the following mAbs from BD Biosciences were used following standard techniques as described above: anti-CD3, anti-CD4, and anti-CD25.
T20963 42513-42621 Sentence denotes Anti-FcγRII/III antibody (2.4G2, ATCC) was included in all stainings to reduce nonspecific antibody binding.
T20964 42622-42797 Sentence denotes To isolate naive murine CD4 T cells from murine DO11.10 or DO11.10xCD2-GATA3 T cells, cells were stained with anti-CD25-FITC, anti-CD62L-PE, and anti-CD4-APC prior to sorting.
T20965 42798-42866 Sentence denotes Dead cells were excluded with 4′',6-Diamidino-2-phenylindole (DAPI).
T20966 42867-43140 Sentence denotes To analyze murine Foxp3 expression in inducible Treg cultures, cells were stained intracellularly with anti-Foxp3-PE according to manufacturer's instruction, in conjunction with anti-CD4-APC and LIVE/DEAD fixable dead cell stain kit (Invitrogen) to discriminate live cells.
T20967 43141-43243 Sentence denotes All monoclonal antibodies for murine cell stainings were purchased from eBioscience or BD Biosciences.
T21784 43245-43262 Sentence denotes Western blotting.
T21785 43263-43448 Sentence denotes For FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen).
T21786 43449-43527 Sentence denotes The proteins were electroblotted onto a PVDF membrane (Amersham Life Science).
T21787 43528-43831 Sentence denotes Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji).
T21788 43832-43900 Sentence denotes Membranes were incubated in stripping buffer and re-blocked for 1 h.
T21789 43901-44151 Sentence denotes The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),
T22255 44153-44204 Sentence denotes Administration of cytokines and antibodies in vivo.
T22256 44205-44444 Sentence denotes Age- and gender-matched normal B6 mice received every other day intraperitoneal (ip) injections of PBS, 1.5 μg rmIL-4, 50 μg anti-IL-4 mAb (11B11 or MAB404), or a mixture of 1.5 μg rmIL-4 plus 50 μg anti-IL-4 mAb (11B11 or MAB404) for 7 d.
T22257 44445-44551 Sentence denotes Thereafter, spleen and lymph node cells were analyzed by flow cytometry for CD3, CD4, and CD25 expression.
T22258 44552-44682 Sentence denotes The anti-mouse IL-4 mAb MAB404 was obtained from R&D Systems, the second anti-mouse IL-4 mAb 11B11 was purchased from eBioscience.
T22565 44684-44689 Sentence denotes ChIP.
T22566 44690-44900 Sentence denotes ChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature.
T22567 44901-45015 Sentence denotes The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin).
T22568 45016-45174 Sentence denotes The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h.
T22569 45175-45409 Sentence denotes Washing and elution buffers were used according to the protocol of Upstate Biotechnology. Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction.
T22570 45410-45463 Sentence denotes The amount of DNA was determined by conventional PCR.
T22571 45464-45571 Sentence denotes The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers:
T22572 45572-45633 Sentence denotes 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′.
T22573 45634-45697 Sentence denotes The PCR products were visualized using an ethidium bromide gel.
T22967 45699-45715 Sentence denotes Pull-down assay.
T22968 45716-45980 Sentence denotes CD4+ T cells were stimulated with PMA and ionomycin for 2 h at 37°C. The cells were pelleted, resuspended in buffer C (20 mM HEPES [pH 7.9], 420 mM NaCl, 1.5 mM MgCl2, 0.2 mM EDTA, 1 mM DTT, protease inhibitors [Sigma]. and 0.1% NP-40) and lysed on ice for 15 min.
T22969 45981-46030 Sentence denotes Insoluble material was removed by centrifugation.
T22970 46031-46109 Sentence denotes The supernatant was diluted 1:3 with buffer D (as buffer C, but without NaCl).
T22971 46110-46494 Sentence denotes The lysates were incubated with 10 μg of poly(dI-dC) (Sigma) and 70 μl of streptavidin-agarose (Amersham Biosciences) carrying biotinylated oligonucleotides, for 3 h at 4 °C. The beads were washed twice with buffer C:D (1:3) and resuspended in DTT-containing loading buffer (NuPAGE; Invitrogen), heated to 70 °C for 10 min, and the eluants on a NuPAGE 4–12% bis-tris gel (Invitrogen).
T22972 46495-46636 Sentence denotes The proteins were electroblotted onto a PVDF membrane (Amersham Biosciences) and detected using an anti-GATA3 mAb (Santa Cruz Biotechnology).
T22973 46637-46701 Sentence denotes Accumulated signals were analyzed using AIDA software (Raytest).
T23457 7387-7427 Sentence denotes Th2 Cells Cannot Induce FOXP3 Expression
T23458 7428-7548 Sentence denotes (A) Human T cells were activated with plate-bound anti-CD3/CD28 with or without TGF-β as indicated on the x-axis of (B).
T23459 7549-7630 Sentence denotes Cells were harvested after 5 days and FOXP3 mRNA was quantified by real-time PCR.
T23460 7631-7684 Sentence denotes Bars show the mean ± SD of 4 independent experiments.
T23461 7685-7806 Sentence denotes (B) In vitro differentiated Th1, Th2, or iTreg cells were activated with anti-CD3/CD28, TGF-β, or anti-IL-4 as indicated.
T23462 7807-7897 Sentence denotes The phenotype of these cells was confirmed by FACS and proliferation analysis (Figure S1).
T23463 7898-7954 Sentence denotes Bars show the mean ± SD of four independent experiments.
T23808 10140-10170 Sentence denotes Th2 Cells Do Not Express FOXP3
T23809 10171-10348 Sentence denotes (A) Intracellular FACS analysis of FOXP3 expression in Th1, Th2, or iTreg-differentiated cells (two rounds, phenotype see Figure S1), rested or activated, with or without TGF-β.
T23810 10349-10400 Sentence denotes FOXP3 expression was measured after 5 d in culture.
T23811 10401-10467 Sentence denotes The dot blots are representative of three independent experiments.
T23812 10468-10555 Sentence denotes (B) Shows the same experimental setup, but naturally occurring Th2 cells were analyzed.
T23813 10556-10613 Sentence denotes Data are representative of three independent experiments.
T24125 10624-10663 Sentence denotes Th2- or IL-4–Producing Cells Lack FOXP3
T24126 10664-10761 Sentence denotes (A) FACS analysis of intracellular FOXP3 and IL-4 expression following PMA/Ionomycin stimulation.
T24127 10762-10999 Sentence denotes CD4+ T cells were gated on the basis of CD45RO and CD25 surface expression (upper panel), and gated cells are shown below for the CD45RO+CD25− (A, left panel), the CD45RO+CD25+ (right panel), and the CD45RO−CD25− subsets (central panel).
T24128 11000-11109 Sentence denotes A statistical analysis of eight independent donors after subtraction of the isotype control are shown in (B).
T24129 11110-11165 Sentence denotes The dotted gray line indicates the IC background level.
T24130 11166-11343 Sentence denotes The error bars show the error of the mean. (C) Similarly, a Th2 clone (BR8), CRTH2+ Th2 cells, IL-4-secreting cells, and memory T cells (CD45RO) were stained for FOXP3 and IL-4.
T24131 11344-11401 Sentence denotes Data are representative of three independent experiments.
T24699 13213-13263 Sentence denotes FOXP3 Induction During the Differentiation Process
T24700 13264-13394 Sentence denotes (A) Human CD4+CD45RA+ T cells were activated with plate-bound anti-CD3/CD28 in the presence of TGF-β (5 ng/ml) or IL-4 (25 ng/ml).
T24701 13395-13518 Sentence denotes The cells were harvested at different time points, and mRNA was quantified by real-time PCR for FOXP3 and GATA3 expression.
T24702 13519-13576 Sentence denotes Bars show the mean ± SD of three independent experiments.
T24703 13578-13725 Sentence denotes (B) Intracellular GATA3 and FOXP3 staining is shown after exposure of CD4+CD45RA+ T cells to differentiating conditions as in part A of the figure.
T24704 13726-13783 Sentence denotes Data are representative of three independent experiments.
T25148 16498-16531 Sentence denotes Effect of IL-4 on FOXP3 Induction
T25149 16532-16720 Sentence denotes (A) A statistical analysis was performed with six donors on day 5 (TGF-β (10 ng/ml) and with or without IL-4 (100 ng/ml)); Shown is the mean, and error bars indicated the SD of six donors.
T25150 16721-16779 Sentence denotes Statistical analysis was performed using the Dunnett test.
T25151 16780-16864 Sentence denotes Statistical significance is indicated by asterisks (*p ≤ 0.05, **p ≤ 0.01, Dunnett).
T25152 16865-17020 Sentence denotes (B) CD4+CD45RA+ cells were activated in the presence of a constant concentration of TGF-β (5 ng/ml) with an increasing concentration of IL-4, as indicated.
T25153 17021-17076 Sentence denotes Cells were harvested for mRNA quantification after 5 d.
T25154 17077-17207 Sentence denotes (C) CD4+CD45RA+ cells were stimulated in vitro with plate-bound anti-CD3/CD28, TGF-β (10ng/ml), and IL-4 (100 ng/ml) as indicated.
T25155 17208-17310 Sentence denotes After 1 h, cell lysates were prepared and analyzed by Western blot for phosphorylated SMAD2 and STAT6.
T25156 17311-17360 Sentence denotes Total STAT6 and GAPDH served as internal control.
T25157 17361-17543 Sentence denotes (D) Intracellular GATA3 and FOXP3 staining are shown after exposure of CD4+CD45RA+ T cells to IL-4 as described for panel B. Data are representative of three independent experiments.
T25857 18665-18710 Sentence denotes IL-4 Inhibits TGF-β–Mediated iTreg Commitment
T25858 18711-18819 Sentence denotes CFSE-labeled CD4+CD45RA+ cells were activated with plate-bound anti-CD3/CD28, TGF-β, and IL-4, as indicated.
T25859 18820-18953 Sentence denotes After 5 d, cells were analyzed by flow cytometry (A) and results of six independent experiments are shown in the bar graph below (B).
T25860 18954-19219 Sentence denotes Statistical significance (one-way Anova, Newman-Keuls) is indicated by asterisks (**p ≤ 0.01, ***p ≤ 0.001). (C) Kinetic analysis of intracellular GATA3 and FOXP3 staining is shown in panel C following exposure of CD4+CD45RA+ T cells to anti-CD3/28, IL-4 and TGF-β.
T25861 19220-19277 Sentence denotes Data are representative of three independent experiments.
T26337 22450-22504 Sentence denotes GATA3 Acts as a Negative Regulator of FOXP3 Expression
T26338 22505-22713 Sentence denotes (A) Human naive CD4+CDRA+ T cells were transduced with 0, 20, 100, and 500 nM of TAT-GATA3 protein, and intracellular presence of GATA3 was analyzed using FACS following anti-CD3/CD28 activation of the cells.
T26339 22714-22840 Sentence denotes GFP-positive cells were gated and analyzed for intracellular FOXP3 expression following a 2-d incubation period (lower panel).
T26340 22841-22897 Sentence denotes Data are representative of four independent experiments.
T26341 22898-23014 Sentence denotes (B) CD4+CD25− T cells were isolated from D011.10 and D011.10xCD2-GATA3 mice and treated with OVA and TGF-β for 96 h.
T26342 23015-23073 Sentence denotes Surface CD4 and intracellular FOXP3 were measured by FACS.
T26343 23074-23137 Sentence denotes These data are representative of three independent experiments.
T26344 23138-23257 Sentence denotes (C) The cells treated as in (B) were harvested and mRNA was quantified by real-time PCR for SMAD7 and TGF-β expression.
T26345 23258-23315 Sentence denotes Bars show the mean ± SD of three independent experiments.
T26927 25937-25977 Sentence denotes GATA3 Represses the Human FOXP3 Promoter
T26928 25978-26176 Sentence denotes (A) Jurkat, Th2 cells, and human primary CD4 cells were transfected with an empty vector (pGL3 basic) or a vector containing the putative FOXP3 promoter region fused to the luciferase reporter gene.
T26929 26177-26330 Sentence denotes Bars show the mean ± SD of arbitrary light units normalized for renilla luciferase of four independent experiments; samples were measured in triplicates.
T26930 26331-26472 Sentence denotes (B) Naive CD4 T cells were transfected with the FOXP3 promoter reporter construct together with a GATA3 expression vector or an empty vector.
T26931 26473-26530 Sentence denotes Bars show the mean ± SD of three independent experiments.
T26932 26531-26716 Sentence denotes (C) Naive (left panel) or memory (right panel) CD4 T cells were transfected with wild-type or a GATA3 mutated 511-FOXP3 promoter reporter construct and activated with PMA and ionomycin.
T26933 26717-26871 Sentence denotes Bars show the mean ± SD of arbitrary light units normalized for renilla luciferase of eight independent experiments; samples were measured in triplicates.
T26934 26872-27063 Sentence denotes (D) Nuclear extracts were prepared from HEK cells transfected with GATA3 or an empty vector, (E) Th1, Th2, or iTreg cells and binding factors precipitated using biotinylated oligonucleotides.
T26935 27064-27149 Sentence denotes The oligonucleotides–transcription factor complexes were separated on a SDS-PAGE gel.
T26936 27150-27251 Sentence denotes The amounts of GATA3 protein in the precipitates were assessed by immunoblotting with anti-GATA3 mAb.
T26937 27252-27301 Sentence denotes Total nuclear extracts were also run as controls.
T26938 27302-27357 Sentence denotes Data are representative of three different experiments.
T26939 27358-27443 Sentence denotes (F) iTreg or Th2 cells were analyzed by ChIP for GATA3 binding to the FOXP3 promoter.
T26940 27444-27551 Sentence denotes The “input” represents PCR amplification of the total sample, which was not subjected to any precipitation.
T26941 27552-27612 Sentence denotes Results are representative of three independent experiments.
T1 0-147 Sentence denotes GATA3-Driven Th2 Responses Inhibit TGF-β1–Induced FOXP3 Expression and the Formation of Regulatory T Cells GATA3 Inhibits FOXP3 and Inducible Tregs
T2 150-158 Sentence denotes Abstract
T3 159-387 Sentence denotes Transcription factors act in concert to induce lineage commitment towards Th1, Th2, or T regulatory (Treg) cells, and their counter-regulatory mechanisms were shown to be critical for polarization between Th1 and Th2 phenotypes.
T4 388-575 Sentence denotes FOXP3 is an essential transcription factor for natural, thymus-derived (nTreg) and inducible Treg (iTreg) commitment; however, the mechanisms regulating its expression are as yet unknown.
T5 576-686 Sentence denotes We describe a mechanism controlling iTreg polarization, which is overruled by the Th2 differentiation pathway.
T6 687-782 Sentence denotes We demonstrated that interleukin 4 (IL-4) present at the time of T cell priming inhibits FOXP3.
T7 783-1004 Sentence denotes This inhibitory mechanism was also confirmed in Th2 cells and in T cells of transgenic mice overexpressing GATA-3 in T cells, which are shown to be deficient in transforming growth factor (TGF)-β–mediated FOXP3 induction.
T8 1005-1127 Sentence denotes This inhibition is mediated by direct binding of GATA3 to the FOXP3 promoter, which represses its transactivation process.
T9 1128-1244 Sentence denotes Therefore, this study provides a new understanding of tolerance development, controlled by a type 2 immune response.
T10 1245-1493 Sentence denotes IL-4 treatment in mice reduces iTreg cell frequency, highlighting that therapeutic approaches that target IL-4 or GATA3 might provide new preventive strategies facilitating tolerance induction particularly in Th2-mediated diseases, such as allergy.
T11 1495-1509 Sentence denotes Author Summary
T12 1511-1724 Sentence denotes Specific immune responses against foreign or autologous antigens are driven by specialized epitope-specific T cells, whose numbers expand upon recognition of antigen found on professional antigen-presenting cells.
T13 1725-1944 Sentence denotes The subsequent maturation process involves the differentiation of certain T cell phenotypes such as pro-inflammatory cells (Th1, Th2, Th17) or regulatory T (Treg) cells, which serve to keep the immune response in check.
T14 1945-2077 Sentence denotes The current study focuses on the role of two key transcription factors—FOXP3 and GATA3—in controlling the commitment of these cells.
T15 2078-2209 Sentence denotes We demonstrate that the Th2 cytokine IL-4 inhibits the induction of FOXP3 and thus inhibits the generation of inducible Treg cells.
T16 2210-2328 Sentence denotes We show that IL-4–induced GATA3 mediates FOXP3 inhibition by directly binding to a GATA element in the FOXP3 promoter.
T17 2329-2544 Sentence denotes We hypothesize that therapeutic agents aimed at neutralizing IL-4 could be a novel strategy to facilitate inducible Treg cell generation and thus promotion of tolerance in allergies and other Th2-dominated diseases.
T18 2546-2619 Sentence denotes It is shown that Th2 responses prevent the generation of inducible Tregs.
T19 2620-2722 Sentence denotes This is mediated by IL-4 induction of GATA3, which binds directly to and represses the FOXP3 promoter.
T20 2723-2834 Sentence denotes This mechanism is likely to be relevant in the induction of immunotolerance, particularly in allergic diseases.
T21 2836-2848 Sentence denotes Introduction
T22 2849-2997 Sentence denotes Effective immune responses are characterized by T cell activation, which directs adaptive and innate immune responses to kill pathogens efficiently.
T23 2998-3181 Sentence denotes Dependent on the pathogen, T cells differentiate into different subtypes, such as Th1 or Th2 cells, which are most efficient in defeating microbial or parasitic invaders respectively.
T24 3182-3341 Sentence denotes A hallmark of Th1 and Th2 differentiation pathways is the exclusiveness of the individual phenotype leading to either Th1 or Th2, but not to mixed populations.
T25 3342-3482 Sentence denotes The exclusiveness of this mechanism is provided by a polarization process, where Th2 differentiation inhibits Th1 commitment and vice versa.
T26 3483-3619 Sentence denotes Specifically interleukin 4 (IL-4)–induced STAT6 and GATA3 inhibit differentiation into Th1 cells in the early phase of commitment [1,2].
T27 3620-3819 Sentence denotes GATA3 is sufficient to induce a Th2 phenotype [3] and acts not only through the induction of IL-4, IL-5 and IL-13, the Th2 cytokines, but also through the inhibition of Th1 cell-specific factors [3].
T28 3820-4074 Sentence denotes Recently, it was shown that T-bet directly modulates GATA3 function, suggesting that transcription factors compete in the early differentiation phase of T cells, potentially integrating environmental signals to finally imprint the T cell phenotype [4,5].
T29 4075-4250 Sentence denotes A GATA3-dominated immune response has been shown to be essential in airway hyperresonsiveness [6] and IL-4–dominated responses can break antigen-specific immune tolerance [7].
T30 4251-4430 Sentence denotes Overexpression of a dominant negative form of GATA3 [8] or treatment with antisense-mediated GATA3 blockade [9] decreased the severity of the allergic airway hyper-responsiveness.
T31 4431-4570 Sentence denotes The discovery of regulatory T (Treg) cells highlights another phenotype of T cells, which is essential for tolerance against self-antigens.
T32 4571-4743 Sentence denotes Naturally occurring, thymus-derived Treg (nTreg) cells are generated in the thymus and are assumed to protect against the activity of autoreactive T cells in the periphery.
T33 4744-4903 Sentence denotes These cells express the forkhead transcription factor FOXP3 and constitutively express CD25 on their surface, but they lack expression of Th1 or Th2 cytokines.
T34 4904-5047 Sentence denotes Particularly interesting are those Treg cells that are generated in the periphery and thus are potential targets for therapeutic interventions.
T35 5048-5117 Sentence denotes These induced Treg (iTreg) cells were reported to express FOXP3 [10].
T36 5118-5355 Sentence denotes The exact mechanisms of iTreg generation are unclear, but T cell receptor (TCR) triggering has been shown to induce FOXP3 expression and suppressive cells in human [11,12], however the phenotype appears to be of transient nature [13,14].
T37 5356-5625 Sentence denotes TGF-β has been demonstrated to be important for the persistent induction of these cells in vitro and in vivo, since animals lacking the TGF-βRII on T cells have fewer peripherally iTreg cells [15] and suffer from a T cell–dependent multiorgan inflammatory disease [16].
T38 5626-5791 Sentence denotes Although the effect of TGF-β on natural and inducible Treg cell induction has been demonstrated repeatedly [15,17], its molecular mechanisms remain to be identified.
T39 5792-5963 Sentence denotes The current study provides evidence that GATA3 and FOXP3 play a competitive role in iTreg cell commitment as T-bet and GATA3 for Th1 and Th2 differentiation, respectively.
T40 5964-6097 Sentence denotes We show that GATA3 inhibits FOXP3 induction and that IL-4 limits FOXP3 expression in a GATA3-mediated way, both in vitro and in vivo.
T41 6098-6273 Sentence denotes We also show that GATA3 directly binds to the FOXP3 promoter and thereby prevents the induction of this gene, demonstrating that Th2 differentiation overrules iTreg induction.
T42 6275-6282 Sentence denotes Results
T43 6284-6317 Sentence denotes FOXP3 Induction in T Cell Subsets
T44 6318-6452 Sentence denotes It is assumed that FOXP3 expression can be induced in nonregulatory T cells, which is an important step in iTreg cell differentiation.
T45 6453-6538 Sentence denotes However, it is not known if all CD4+ T cells have the same capacity to express FOXP3.
T46 6539-6918 Sentence denotes To investigate whether FOXP3 can be expressed by any T cell subset or if expression is restricted to a distinct lineage, FOXP3 mRNA expression was analyzed in freshly isolated T cells such as CD25-depleted CD4+ cells, CD45RA+ naive or CD45RO+ memory T cells (Figure 1A), as well as T cells driven in vitro toward Th1, Th2, or iTreg phenotypes (Figure 1B; phenotype on Figure S1).
T47 6919-7074 Sentence denotes The CD4+CD25–, CD45RA+, CD45RO+, and CD4+CD45RO+CD25– were able to significantly induce FOXP3 mRNA up to 30-fold upon TCR activation and addition of TGF-β.
T48 7075-7116 Sentence denotes Th1 cells showed only a 10-fold increase.
T49 7117-7211 Sentence denotes In contrast, Th2 cells stimulated under the same conditions did not increase FOXP3 expression.
T50 7212-7376 Sentence denotes The in vitro generated iTreg cells were unable to further up-regulate FOXP3, which was already at high levels under the resting conditions (Figure 1B, right panel).
T51 7377-7427 Sentence denotes Figure 1 Th2 Cells Cannot Induce FOXP3 Expression
T52 7428-7548 Sentence denotes (A) Human T cells were activated with plate-bound anti-CD3/CD28 with or without TGF-β as indicated on the x-axis of (B).
T53 7549-7630 Sentence denotes Cells were harvested after 5 days and FOXP3 mRNA was quantified by real-time PCR.
T54 7631-7684 Sentence denotes Bars show the mean ± SD of 4 independent experiments.
T55 7685-7806 Sentence denotes (B) In vitro differentiated Th1, Th2, or iTreg cells were activated with anti-CD3/CD28, TGF-β, or anti-IL-4 as indicated.
T56 7807-7897 Sentence denotes The phenotype of these cells was confirmed by FACS and proliferation analysis (Figure S1).
T57 7898-7954 Sentence denotes Bars show the mean ± SD of four independent experiments.
T58 7957-8083 Sentence denotes Th2 cells are known to produce IL-4 upon activation, which may interact with TGF-β signaling and thus prevent FOXP3 induction.
T59 8084-8233 Sentence denotes However, the neutralization of IL-4 with a blocking IL-4 antibody did not rescue FOXP3 expression in the differentiated Th2 cells (unpublished data).
T60 8234-8326 Sentence denotes These data demonstrated that Th2 cells have a limited capacity to express FOXP3 (Figure 1B).
T61 8327-8477 Sentence denotes The inability of Th2 cells to express FOXP3 was also documented at the single-cell level, confirming that Th2 cells lack FOXP3 expression (Figure 2A).
T62 8478-8533 Sentence denotes Only iTreg cells expressed FOXP3 in resting conditions.
T63 8534-8637 Sentence denotes Interestingly, we observed that resting iTreg cells express FOXP3 but show low CD25 surface expression.
T64 8638-8786 Sentence denotes Repeated exposure to TGF-β did not further increase the FOXP3 expression in the iTreg lineage but transiently induced FOXP3 expression in Th1 cells.
T65 8787-8980 Sentence denotes Naturally occurring Th2 cells such as CRTH2+ T cells, T cells isolated according to their IL-4 secretion, or an IL-4–producing T cell clone (BR8) were also lacking FOXP3 expression (Figure 2B).
T66 8981-9091 Sentence denotes Furthermore TGF-β–mediated FOXP3 induction failed in these cells in contrast to the naive T cells (Figure 2B).
T67 9092-9255 Sentence denotes Because IL-4 is the key Th2 cytokine, the expression of IL-4 and FOXP3 in freshly isolated CD4+ T cells was analyzed by fluorescence activated cell sorting (FACS).
T68 9256-9378 Sentence denotes IL-4–expressing cells were most abundant among CD45RO+CD25– cells, which did not co-express FOXP3 (Figure 3A, left panel).
T69 9379-9483 Sentence denotes In contrast, CD45RO+CD25+ cells abundantly expressed FOXP3, while lacking IL-4 (Figure 3A, right panel).
T70 9484-9604 Sentence denotes As shown in Figure 3B, the frequency of the IL-4+ cells was always below 1% in the FOXP3+ cells close to the background.
T71 9605-9740 Sentence denotes The IL-4+ cells were confined to the FOXP3– cells, as shown for the CD45RO+CD25–, CD45RO+CD25+, and CD45RO+CD25+high cells (Figure 3B).
T72 9741-9840 Sentence denotes In addition, neither the Th2 clone (BR8) nor CRTH2 cells significantly expressed FOXP3 (Figure 3C).
T73 9841-10013 Sentence denotes Cells enriched for their IL-4 secretion using the magnetic cell isolation technology contained some FOXP3-expressing cells, but importantly, the expression did not overlap.
T74 10014-10129 Sentence denotes Taken together, these data indicate that FOXP3 was not expressed by Th2 cells and was not inducible in those cells.
T75 10130-10170 Sentence denotes Figure 2 Th2 Cells Do Not Express FOXP3
T76 10171-10348 Sentence denotes (A) Intracellular FACS analysis of FOXP3 expression in Th1, Th2, or iTreg-differentiated cells (two rounds, phenotype see Figure S1), rested or activated, with or without TGF-β.
T77 10349-10400 Sentence denotes FOXP3 expression was measured after 5 d in culture.
T78 10401-10467 Sentence denotes The dot blots are representative of three independent experiments.
T79 10468-10555 Sentence denotes (B) Shows the same experimental setup, but naturally occurring Th2 cells were analyzed.
T80 10556-10613 Sentence denotes Data are representative of three independent experiments.
T81 10614-10663 Sentence denotes Figure 3 Th2- or IL-4–Producing Cells Lack FOXP3
T82 10664-10761 Sentence denotes (A) FACS analysis of intracellular FOXP3 and IL-4 expression following PMA/Ionomycin stimulation.
T83 10762-10999 Sentence denotes CD4+ T cells were gated on the basis of CD45RO and CD25 surface expression (upper panel), and gated cells are shown below for the CD45RO+CD25− (A, left panel), the CD45RO+CD25+ (right panel), and the CD45RO−CD25− subsets (central panel).
T84 11000-11109 Sentence denotes A statistical analysis of eight independent donors after subtraction of the isotype control are shown in (B).
T85 11110-11165 Sentence denotes The dotted gray line indicates the IC background level.
T86 11166-11343 Sentence denotes The error bars show the error of the mean. (C) Similarly, a Th2 clone (BR8), CRTH2+ Th2 cells, IL-4-secreting cells, and memory T cells (CD45RO) were stained for FOXP3 and IL-4.
T87 11344-11401 Sentence denotes Data are representative of three independent experiments.
T88 11403-11452 Sentence denotes FOXP3 and GATA3 Kinetics in Differentiating Cells
T89 11453-11623 Sentence denotes The limited capacity of differentiated effector cells to induce FOXP3 expression suggests that iTreg induction has to occur before effector T cell differentiation occurs.
T90 11624-11814 Sentence denotes Therefore, we analyzed the expression of FOXP3 and GATA3 during the differentiation of naive CD4 T cells into Th0 (neutral, anti-IL-4, anti-IFN-γ, and anti IL-12), Th2, and iTreg phenotypes.
T91 11815-12000 Sentence denotes After initiation of the differentiation process, FOXP3 and GATA3 showed a similar expression kinetic within the first 3 d, which are considered to be critical in T cell commitment [18].
T92 12001-12111 Sentence denotes Under Th2 differentiation conditions, FOXP3 mRNA expression increased only marginally (Figure 4A, left panel).
T93 12112-12322 Sentence denotes Thus, although GATA3 and FOXP3 showed similar kinetics, their expression polarizes at the end of the differentiation process when cells were cultured towards Th2 or iTreg cells, respectively (Figure 4A and 4B).
T94 12323-12633 Sentence denotes Interestingly, the Th0 cells were expressing more FOXP3 than the Th2 cells, but expressed low levels of GATA3; however, the protein expression slightly differed from mRNA expression, suggesting also posttranslational regulation and degradation as potential additional mechanisms in the differentiation process.
T95 12634-12798 Sentence denotes The phenotype of iTreg cells included an anergic phenotype upon anti-CD3 re-stimulation (Figure S1A), CD103, CTLA-4, GITR, and PD-1 surface expression (Figure S1B).
T96 12799-13018 Sentence denotes On the single-cell level, it can be seen that cells progress through a transition phase, where GATA3 and FOXP3 expression coexist to some degree in the same cells, which is resolved in iTreg cells after 7 d (Figure 4B).
T97 13019-13202 Sentence denotes Taken together, these data demonstrated that Th2 cells have lost their capacity to express FOXP3 and showed that Th2 and iTreg cells arise from two different differentiation pathways.
T98 13203-13263 Sentence denotes Figure 4 FOXP3 Induction During the Differentiation Process
T99 13264-13394 Sentence denotes (A) Human CD4+CD45RA+ T cells were activated with plate-bound anti-CD3/CD28 in the presence of TGF-β (5 ng/ml) or IL-4 (25 ng/ml).
T100 13395-13518 Sentence denotes The cells were harvested at different time points, and mRNA was quantified by real-time PCR for FOXP3 and GATA3 expression.
T101 13519-13576 Sentence denotes Bars show the mean ± SD of three independent experiments.
T102 13578-13725 Sentence denotes (B) Intracellular GATA3 and FOXP3 staining is shown after exposure of CD4+CD45RA+ T cells to differentiating conditions as in part A of the figure.
T103 13726-13783 Sentence denotes Data are representative of three independent experiments.
T104 13785-13830 Sentence denotes IL-4 Inhibits TGF-β-Mediated iTreg Commitment
T105 13831-13928 Sentence denotes IL-4 induces differentiation of naive T cells, upon antigen encounter, into the Th2 cell lineage.
T106 13929-14041 Sentence denotes We therefore asked whether IL-4 is able to inhibit TGF-β induction of FOXP3 during the priming of naive T cells.
T107 14042-14175 Sentence denotes Human CD4+CD45RA+ T cells were activated with plate-bound anti-CD3/CD28 in the presence of TGF-β and/or IL-4 and harvested after 5 d.
T108 14176-14312 Sentence denotes IL-4 efficiently repressed the TGF-β–mediated induction of FOXP3 expression (Figure 5A) in a concentration-dependent manner (Figure 5B).
T109 14313-14409 Sentence denotes Low levels of GATA3 were induced also in the absence of IL-4, as it was previously observed [3].
T110 14410-14493 Sentence denotes However, at low concentration, IL-4 was able to marginally induce FOXP3 expression.
T111 14494-14597 Sentence denotes Of note, GATA3 was also induced in the presence of TGF-β at high IL-4 concentration (Figure 5A and 5B).
T112 14598-14914 Sentence denotes The IL-4–mediated prevention of FOXP3 expression was not caused by interferences of the receptor signaling, because the phosphorylation of SMAD2 or STAT6 was not affected by the addition of IL-4 and/or TGF-β, which demonstrates that IL-4 as well as TGF-β signaling were functional under these conditions (Figure 5C).
T113 14915-15052 Sentence denotes Increasing amounts of IL-4 increase intracellular GATA3, whereas FOXP3 decreased, which is consistent with the mRNA analysis (Figure 5D).
T114 15053-15162 Sentence denotes Furthermore, injection of IL-4 into wild-type B6 mice decreased the inducible or natural Treg number in vivo.
T115 15163-15279 Sentence denotes A distinction of the Treg subsets is not possible, because recently activated iTreg cells also express surface CD25.
T116 15280-15466 Sentence denotes We used complexes of recombinant mouse IL-4 (rmIL-4) plus anti-IL-4 monoclonal antibodies (mAbs), which have been shown to dramatically increase the potency of the cytokine in vivo [19].
T117 15467-15622 Sentence denotes In these mice, the percentage of CD4+CD25+ and FOXP3+ T cells dramatically decreased when the antibody-cytokine immune complexes were injected (Figure S2).
T118 15623-15921 Sentence denotes Upon administration of rmIL-4 plus anti-IL-4 mAb complexes, the total number of CD4+CD25+ T cell, as well as the Foxp3+ T cells diminished by half (Figure S2G and S2H), confirming that the lower percentage was not due to an increase in the CD4+CD25– cells, but a real decrease of CD4+CD25+ T cells.
T119 15922-16042 Sentence denotes In conclusion, IL-4 negatively regulates the natural or inducible Treg cell turnover not only in vitro but also in vivo.
T120 16043-16240 Sentence denotes To study the effects of IL-4 on already-existing human natural or inducible Treg cells, we exposed sorted CD25+ T cells (nTreg cells) to IL-4 and analyzed FOXP3 expression and suppressive capacity.
T121 16241-16385 Sentence denotes In already-existing Treg cells, IL-4 failed to inhibit FOXP3 expression (Figure S3A), and the suppressive capacity was not altered (Figure S3C).
T122 16386-16487 Sentence denotes Similarly pre-existing iTreg cells did not decrease FOXP3 expression upon IL-4 exposure (Figure S3B).
T123 16488-16531 Sentence denotes Figure 5 Effect of IL-4 on FOXP3 Induction
T124 16532-16720 Sentence denotes (A) A statistical analysis was performed with six donors on day 5 (TGF-β (10 ng/ml) and with or without IL-4 (100 ng/ml)); Shown is the mean, and error bars indicated the SD of six donors.
T125 16721-16779 Sentence denotes Statistical analysis was performed using the Dunnett test.
T126 16780-16864 Sentence denotes Statistical significance is indicated by asterisks (*p ≤ 0.05, **p ≤ 0.01, Dunnett).
T127 16865-17020 Sentence denotes (B) CD4+CD45RA+ cells were activated in the presence of a constant concentration of TGF-β (5 ng/ml) with an increasing concentration of IL-4, as indicated.
T128 17021-17076 Sentence denotes Cells were harvested for mRNA quantification after 5 d.
T129 17077-17207 Sentence denotes (C) CD4+CD45RA+ cells were stimulated in vitro with plate-bound anti-CD3/CD28, TGF-β (10ng/ml), and IL-4 (100 ng/ml) as indicated.
T130 17208-17310 Sentence denotes After 1 h, cell lysates were prepared and analyzed by Western blot for phosphorylated SMAD2 and STAT6.
T131 17311-17360 Sentence denotes Total STAT6 and GAPDH served as internal control.
T132 17361-17485 Sentence denotes (D) Intracellular GATA3 and FOXP3 staining are shown after exposure of CD4+CD45RA+ T cells to IL-4 as described for panel B.
T133 17486-17543 Sentence denotes Data are representative of three independent experiments.
T134 17545-17588 Sentence denotes The Role of TGF-β in T Cell Differentiation
T135 17589-17816 Sentence denotes Although TGF-β–reduced CD25, IL-4 expression, and CD25 expression (Figure 6B), IL-4 significantly inhibited TGF-β–mediated induction of FOXP3 in naive T cells driven toward FOXP3+ T cells, as shown by FACS analysis (Figure 6A).
T136 17817-17981 Sentence denotes It is known that IL-4 is a potent growth factor and may therefore favor the proliferation of FOXP3– cells and thus decrease the relative percentage of FOXP3+ cells.
T137 17982-18139 Sentence denotes However, analysis of cell division kinetics by CFSE-labeling demonstrated that IL-4 did not differentially promote cell growth of FOXP3+ over that of FOXP3–.
T138 18140-18217 Sentence denotes In fact both populations showed similarly enhanced proliferation (Figure 6A).
T139 18218-18507 Sentence denotes Furthermore the TGF-β–mediated induction of FOXP3 expression was not caused by overgrowth of a CD25–FOXP3+ minority, since the number of FOXP3+ cells was low/absent in the purified CD4+CD45RA+ T cells (between 0% and 1%), and the FOXP3+ cells were not confined to the highly divided cells.
T140 18508-18654 Sentence denotes CD25 was down-regulated in TGF-β–treated cells compare to activated T cells, which was even more pronounced in cells cultured with TGF-β and IL-4.
T141 18655-18710 Sentence denotes Figure 6 IL-4 Inhibits TGF-β–Mediated iTreg Commitment
T142 18711-18819 Sentence denotes CFSE-labeled CD4+CD45RA+ cells were activated with plate-bound anti-CD3/CD28, TGF-β, and IL-4, as indicated.
T143 18820-18953 Sentence denotes After 5 d, cells were analyzed by flow cytometry (A) and results of six independent experiments are shown in the bar graph below (B).
T144 18954-19219 Sentence denotes Statistical significance (one-way Anova, Newman-Keuls) is indicated by asterisks (**p ≤ 0.01, ***p ≤ 0.001). (C) Kinetic analysis of intracellular GATA3 and FOXP3 staining is shown in panel C following exposure of CD4+CD45RA+ T cells to anti-CD3/28, IL-4 and TGF-β.
T145 19220-19277 Sentence denotes Data are representative of three independent experiments.
T146 19280-19378 Sentence denotes The addition of IL-4 to iTreg-driving conditions decreased the number of FOXP3+ cells (Figure 6B).
T147 19379-19502 Sentence denotes In line with the previous findings, the IL-4–producing cells and the FOXP3 expressing cells are nonoverlapping populations.
T148 19503-19624 Sentence denotes Since FOXP3 is known to act as a repressor of cytokine expression [20], we therefore analyzed GATA3 and FOXP3 expression.
T149 19625-20016 Sentence denotes The expression kinetic of naive T cells exposed to IL-4 and TGF-β demonstrated that GATA3 and FOXP3 are initially found in separate populations (day 2), but transiently co-express both factors (days 4–8), before establishing separate populations at the end of the differentiation process (day 10; Figure 6C), suggesting that GATA3 inhibits the development of iTreg cells by repressing FOXP3.
T150 20017-20240 Sentence denotes These results showed that IL-4 acts in vitro as an inhibitor of FOXP3 expression, without interfering with TGF-β signaling, probably acting at the level of transcription factors, and possibly by a GATA3-dependent mechanism.
T151 20242-20291 Sentence denotes GATA3 Is a Negative Regulator of FOXP3 Expression
T152 20292-20424 Sentence denotes FOXP3 expression decreased once GATA3 expression is high; therefore, we hypothesized a potential role for GATA3 in repressing FOXP3.
T153 20425-20668 Sentence denotes Besides GATA3′s well-known positive effect on gene regulation, GATA3′s repressive capabilities were previously shown to restrict Th1 commitment by inhibiting STAT4 expression [2,21], and therefore GATA3 prevents differentiation into Th1 cells.
T154 20669-20894 Sentence denotes To investigate whether GATA3 can directly inhibit FOXP3 induction, we transduced GATA3 or a truncated GATA3 lacking the DNA-binding domain in human primary CD4+CD45RA+ T cells using a TAT-fused, recombinantly expressed GATA3.
T155 20895-21003 Sentence denotes After transduction, the cells were activated with soluble anti-CD3/CD28 in the presence or absence of TGF-β.
T156 21004-21134 Sentence denotes TAT-GATA3 was successfully transduced in a homogeneous and dose-dependent manner into human CD4+ T cells (Figure 7A, upper panel).
T157 21135-21356 Sentence denotes TAT-GATA3 reduced FOXP3 expression in a dose-dependent manner, whereas a DNA-binding domain truncated version (TAT-ΔDBD-GATA3) did not affect FOXP3 expression as compared with expression in untransduced cells (Figure 7A).
T158 21357-21556 Sentence denotes In addition, we analyzed the inhibitory effect of GATA3 on FOXP3 in transgenic DO11.10 mice, constitutively overexpressing GATA3 under the control of the CD2 locus control region (DO11.10xCD2-GATA3).
T159 21557-21670 Sentence denotes The thymic selection into the CD4 lineage is largely intact in DO11.10xCD2-GATA3 (RW Hendriks, unpublished data).
T160 21671-21773 Sentence denotes These mice develop lymphomas at an older age, but signs of autoimmune disease were not described [22].
T161 21774-21956 Sentence denotes To investigate the effect of GATA3 on iTreg, CD4+CD62L+CD25– cells were isolated, activated with OVA in the presence or absence of TGF-β, and Foxp3 expression was analyzed after 4 d.
T162 21957-22014 Sentence denotes The naive CD4+CD25– cells were Foxp3– (unpublished data).
T163 22015-22126 Sentence denotes As described for the human cells, TGF-β dramatically up-regulated Foxp3 in the DO11.10 littermate control mice.
T164 22127-22269 Sentence denotes In contrast, cells from the CD2-GATA3xDO11.10 mice showed dramatically reduced Foxp3 expression when activated with TGF-β and OVA (Figure 7B).
T165 22270-22439 Sentence denotes All mice produced similar amounts of TGF-β; in addition, Smad7 was equally expressed [23] in T cells of both mice strains (Figure 7C), indicating intact TGF-β signaling.
T166 22440-22504 Sentence denotes Figure 7 GATA3 Acts as a Negative Regulator of FOXP3 Expression
T167 22505-22713 Sentence denotes (A) Human naive CD4+CDRA+ T cells were transduced with 0, 20, 100, and 500 nM of TAT-GATA3 protein, and intracellular presence of GATA3 was analyzed using FACS following anti-CD3/CD28 activation of the cells.
T168 22714-22840 Sentence denotes GFP-positive cells were gated and analyzed for intracellular FOXP3 expression following a 2-d incubation period (lower panel).
T169 22841-22897 Sentence denotes Data are representative of four independent experiments.
T170 22898-23014 Sentence denotes (B) CD4+CD25− T cells were isolated from D011.10 and D011.10xCD2-GATA3 mice and treated with OVA and TGF-β for 96 h.
T171 23015-23073 Sentence denotes Surface CD4 and intracellular FOXP3 were measured by FACS.
T172 23074-23137 Sentence denotes These data are representative of three independent experiments.
T173 23138-23257 Sentence denotes (C) The cells treated as in (B) were harvested and mRNA was quantified by real-time PCR for SMAD7 and TGF-β expression.
T174 23258-23315 Sentence denotes Bars show the mean ± SD of three independent experiments.
T175 23318-23455 Sentence denotes Taken together, these results demonstrated a repressive role of IL-4-induced GATA3 transcription factor in the generation of iTreg cells.
T176 23457-23491 Sentence denotes GATA3 Represses the FOXP3 Promoter
T177 23492-23669 Sentence denotes To investigate the molecular mechanism of GATA3-mediated repression of human FOXP3, the human FOXP3 promoter was studied and a palindromic binding site for GATA3 was discovered.
T178 23670-23764 Sentence denotes The GATA-binding site is located 303 bp upstream from the transcription start site (TSS) [24].
T179 23765-23899 Sentence denotes This site is highly conserved between humans, mice, and rats (Figure S4) and may therefore play an important role in FOXP3 regulation.
T180 23900-23988 Sentence denotes The functional relevance of this site was studied using a FOXP3-promoter construct [24].
T181 23989-24190 Sentence denotes We transfected human primary CD4+ T cells, in vitro differentiated Th2 cells, and Jurkat cells (Jurkat cells are known to constitutively express GATA3 [25,26]), and we measured FOXP3 promoter activity.
T182 24191-24406 Sentence denotes The promoter was not active in the GATA3-expressing cell line Jurkat or in the in vitro-differentiated Th2 cells, whereas the construct was active in the CD4 cells, which express a lower amount of GATA3 (Figure 8A).
T183 24407-24546 Sentence denotes Overexpression of GATA3 in naive T cells diminished luciferase activity of the FOXP3 promoter compared with the control vector (Figure 8B).
T184 24547-24667 Sentence denotes To further address the function of the GATA3 site, we inserted a site-specific mutation deleting the GATA3-binding site.
T185 24668-24901 Sentence denotes This mutation increased luciferase activity by 3-fold in memory CD4+CD45RO+ T cells, whereas no difference was observed in naive (GATA3–) CD4+CD45RA+ T cells, revealing a repressor activity of GATA3 on the FOXP3 promoter (Figure 8C).
T186 24902-24992 Sentence denotes Furthermore GATA3 binds directly to the FOXP3 promoter as investigated by pull-down assay.
T187 24993-25246 Sentence denotes HEK cells were transiently transfected with GATA3 or a control vector, and increasing amounts of lysates were incubated with oligonucleotides containing the GATA3 site of the FOXP3 promoter or a control oligonucleotide with a mutated GATA3-binding site.
T188 25247-25311 Sentence denotes After the pull-down, GATA3 binding was detected by Western blot.
T189 25312-25398 Sentence denotes Similarly, GATA3-expressing Th2 cells and iTreg cells were subjected to this approach.
T190 25399-25498 Sentence denotes Only HEK cells overexpressing GATA3 and Th2 cells showed GATA3-binding activity (Figure 8D and 8E).
T191 25499-25578 Sentence denotes These experiments demonstrated that GATA3 binds the palindromic FOXP3 promoter.
T192 25579-25811 Sentence denotes To gain insights into the in vivo situation, we performed a chromatin immunoprecipitation (ChIP) using an anti-GATA3 antibody and showed that GATA3 binds to the FOXP3 promoter region in Th2 cells, but not in iTreg cells (Figure 7F).
T193 25812-25926 Sentence denotes Taken together these data demonstrate that the GATA3-binding to the FOXP3 promoter is repressing FOXP3 expression.
T194 25927-25977 Sentence denotes Figure 8 GATA3 Represses the Human FOXP3 Promoter
T195 25978-26176 Sentence denotes (A) Jurkat, Th2 cells, and human primary CD4 cells were transfected with an empty vector (pGL3 basic) or a vector containing the putative FOXP3 promoter region fused to the luciferase reporter gene.
T196 26177-26330 Sentence denotes Bars show the mean ± SD of arbitrary light units normalized for renilla luciferase of four independent experiments; samples were measured in triplicates.
T197 26331-26472 Sentence denotes (B) Naive CD4 T cells were transfected with the FOXP3 promoter reporter construct together with a GATA3 expression vector or an empty vector.
T198 26473-26530 Sentence denotes Bars show the mean ± SD of three independent experiments.
T199 26531-26716 Sentence denotes (C) Naive (left panel) or memory (right panel) CD4 T cells were transfected with wild-type or a GATA3 mutated 511-FOXP3 promoter reporter construct and activated with PMA and ionomycin.
T200 26717-26871 Sentence denotes Bars show the mean ± SD of arbitrary light units normalized for renilla luciferase of eight independent experiments; samples were measured in triplicates.
T201 26872-27063 Sentence denotes (D) Nuclear extracts were prepared from HEK cells transfected with GATA3 or an empty vector, (E) Th1, Th2, or iTreg cells and binding factors precipitated using biotinylated oligonucleotides.
T202 27064-27149 Sentence denotes The oligonucleotides–transcription factor complexes were separated on a SDS-PAGE gel.
T203 27150-27251 Sentence denotes The amounts of GATA3 protein in the precipitates were assessed by immunoblotting with anti-GATA3 mAb.
T204 27252-27301 Sentence denotes Total nuclear extracts were also run as controls.
T205 27302-27357 Sentence denotes Data are representative of three different experiments.
T206 27358-27443 Sentence denotes (F) iTreg or Th2 cells were analyzed by ChIP for GATA3 binding to the FOXP3 promoter.
T207 27444-27551 Sentence denotes The “input” represents PCR amplification of the total sample, which was not subjected to any precipitation.
T208 27552-27612 Sentence denotes Results are representative of three independent experiments.
T209 27614-27624 Sentence denotes Discussion
T210 27625-27796 Sentence denotes The current study reveals that FOXP3 induction, an important step in iTreg commitment, is inhibited by GATA3, which is the key regulator for polarization toward Th2 cells.
T211 27797-27899 Sentence denotes After differentiation, the effector Th2 cells become refractory to conversion into a FOXP3+ phenotype.
T212 27900-28003 Sentence denotes In accordance with other studies, we found that CD4+CD25– cells were able to up-regulate FOXP3 [12,27].
T213 28004-28200 Sentence denotes Already-committed cells such as memory T cells and Th1 cells showed only moderate and transient FOXP3 induction, which is not sufficient to change the phenotype toward a regulatory T cell profile.
T214 28201-28497 Sentence denotes In contrast, naive T cells could efficiently up-regulate FOXP3 when treated with TGF-β to induce iTreg cells [10,28–34], suggesting that FOXP3 plays an important role in the early differentiation process and may act in a way similar to that known for the Th1/Th2 decision factors T-bet and GATA3.
T215 28498-28754 Sentence denotes This commitment is characterized by competitive expression of these factors [35,36], which we also observed in differentiating FOXP3+ iTreg cells including a phase of co-expression, which turns into nonoverlapping expression upon completed differentiation.
T216 28755-28908 Sentence denotes In this competitive process TGF-β appeared to be mandatory for the induction of FOXP3, possibly by keeping the expression of GATA3 and T-bet low [37,38].
T217 28909-29188 Sentence denotes In contrast, differentiating naive T cells in the absence of polarization factors (Th0) such as IL-4, IL-12, or TGF-β showed only a transient FOXP3 expression and failed to generate a population of FOXP3-expressing cells, but GATA3 and T-bet were up-regulated (unpublished data).
T218 29189-29386 Sentence denotes Interestingly, as we and others previously described, FOXP3-promoting factors, such as dexamethasone [39], CTLA-4 [40], and estrogens [41], are also known as inhibitors of GATA3 expression [42–45].
T219 29387-29518 Sentence denotes Therefore GATA3 not only induces differentiation into Th2 cells but also inhibits FOXP3 expression and commitment into iTreg cells.
T220 29519-29690 Sentence denotes The Th2 cytokine IL-4 but not IL-13 (unpublished data) was able to inhibit TGF-β–mediated FOXP3 induction and therefore prevented conversion into the regulatory phenotype.
T221 29691-29819 Sentence denotes To prove the inhibitory effect of IL-4 on inducible or natural Treg commitment in vivo, we treated mice with IL-4 and anti-IL-4.
T222 29820-29892 Sentence denotes This has been shown to increase the effect of the cytokine in vivo [19].
T223 29893-30035 Sentence denotes Only the IL-4/IL-4 mAb complex resulted in a decrease of the amount of natural or inducible Treg (CD25+ and Foxp3+) cells 7 d after treatment.
T224 30036-30145 Sentence denotes Our results suggest that IL-4 is only interfering with the differentiation of naive T cells into iTreg cells.
T225 30146-30458 Sentence denotes But since a distinction of nTreg and iTreg cells is currently not possible, because iTreg cells also transiently express CD25 after activation, we cannot exclude that IL-4 may also inhibit Foxp3 expression in nTreg cells in vivo or that additional effects may contribute to the observed drop in Foxp3 expression.
T226 30459-30598 Sentence denotes IL-4 has already been shown to negatively regulate the development of naive T cells into Th1 or the IL-17–producing T cells (Th17) [46,47].
T227 30599-30801 Sentence denotes Similar effects have been recently described for IL-6, which, combined with TGF-β, inhibits the generation of iTreg cells and induces differentiation into the Th17 cells by an unknown mechanism [48,49].
T228 30802-30905 Sentence denotes Thus the polarization into iTreg cells is negatively regulated by the effector cytokines IL-4 and IL-6.
T229 30906-31005 Sentence denotes IL-4 has been previously shown to induce the generation of FOXP3+ Treg cells out of CD4+CD25– [50].
T230 31006-31156 Sentence denotes In those experiments, the concentrations of IL-4 used were low, and as we also observed, IL-4 at low concentration slightly enhanced FOXP3 expression.
T231 31157-31238 Sentence denotes Importantly, these concentrations were not sufficient to induce GATA3 expression.
T232 31239-31359 Sentence denotes IL-4 may favor proliferation of nTreg cells [47] or directly regulate FOXP3 expression in a STAT-dependent fashion [51].
T233 31360-31520 Sentence denotes Since IL-13 does not effectively reduce FOXP3 and fails to induce GATA3, we hypothesized that the IL-4–dependent inhibition of FOXP3 could be mediated by GATA3.
T234 31521-31660 Sentence denotes In fact, GATA3-inducing IL-4 concentrations repressed TGF-β–mediated FOXP3 expression, whereas IL-4 as well as TGF-β signaling were intact.
T235 31661-31829 Sentence denotes This result suggested a competitive mechanism between GATA3 and FOXP3 transcription factors in determining lineage commitment during the early phase of differentiation.
T236 31830-31932 Sentence denotes Accordingly, we investigated naturally high GATA3-expressing cells and confirmed the absence of FOXP3.
T237 31933-32049 Sentence denotes Protein transduction of GATA3 into naive T cells inhibited FOXP3 induction in human, differentiating, naive T cells.
T238 32050-32195 Sentence denotes This inhibitory effect of GATA3 was further confirmed in BALB/c transgenic mice, expressing GATA3 in T cells (DO11.10:CD2-GATA3 transgenic mice).
T239 32196-32371 Sentence denotes In line with the transient overexpression of GATA3 in human T cells, cells of these mice failed to induce FOXP3 expression upon exposure with antigen in the presence of TGF-β.
T240 32372-32529 Sentence denotes Strikingly, the DO11.10:CD2-GATA3 mice do have peripheral FOXP3+ cells, which however displayed a 10%–25% lower frequency compared to wild-type DO11.10 mice.
T241 32530-32671 Sentence denotes Thus GATA3 restrains the development of certain Treg subsets, presumably the inducible, peripheral population and not those of thymic origin.
T242 32672-32801 Sentence denotes Thymic T cells undergo a different maturation process, which may explain the insensitivity of nTreg to GATA3 overexpression [52].
T243 32802-33172 Sentence denotes In contrast to the Th2-differentiating and iTreg-inhibiting function of GATA3 in peripheral T cells, GATA3 acts in the thymus together with other transcription factors such as the Repressor of GATA3 (ROG) in the differentiation process toward CD8 cells [53,54] or participates in complex transcriptional feedback network to regulate sympathoadrenal differentiation [55].
T244 33173-33258 Sentence denotes Therefore, the role of GATA3 appears to be tissue specific and cannot be generalized.
T245 33259-33369 Sentence denotes Our study demonstrates that GATA3 repressed FOXP3 expression directly by binding to the FOXP3 promoter region.
T246 33370-33525 Sentence denotes A palindromic GATA-site is located 303 bp upstream of the TSS in a highly conserved region, which we have previously identified as the FOXP3 promoter [24].
T247 33526-33797 Sentence denotes Site-specific mutation of this site increased the activity of the promoter constructs, thus revealing the repressive nature of this GATA element in memory T cells, which naturally express GATA3, whereas no difference was seen in naive T cells, which do not express GATA3.
T248 33798-33891 Sentence denotes This palindromic GATA element is bound by GATA3 protein as proven with pull-down experiments.
T249 33892-33976 Sentence denotes Furthermore, it is shown by ChIP that GATA3 binds this element also in intact cells.
T250 33977-34184 Sentence denotes It is known that GATA3 can induce transcription by chromatin remodeling [56], by directly transactivating promoters [36], or, as shown in the current study, acts as a repressor of gene expression [21,57–59].
T251 34185-34291 Sentence denotes Therefore keeping GATA3 expression low might be required to induce efficient FOXP3+ iTreg cell generation.
T252 34292-34581 Sentence denotes The molecular interactions enabling GATA3 to inhibit FOXP3 are not identified yet, but the GATA-binding site is located adjacent to positive, inducing sites, composed of AP-1-NFATc2 sites [24], and GATA3 may compete with the binding of AP-1/NFAT to the promoter (unpublished observations).
T253 34582-34671 Sentence denotes In summary, we demonstrated that FOXP3 is negatively regulated by cytokines such as IL-4.
T254 34672-34845 Sentence denotes GATA3 acts as an inhibitor of FOXP3 expression in early T cell differentiation, as well as in differentiated Th2 cells by directly binding and repressing the FOXP3 promoter.
T255 34846-34954 Sentence denotes We therefore describe a new mechanism of how Il-4 avoids tolerance induction by repressing FOXP3 expression.
T256 34955-35146 Sentence denotes These findings will give new perspectives toward understanding molecular mechanisms of iTreg induction and thus pathways of peripheral tolerance induction, particularly in allergy and asthma.
T257 35148-35169 Sentence denotes Materials and Methods
T258 35171-35176 Sentence denotes Mice.
T259 35177-35257 Sentence denotes Normal B6 mice were purchased from the Jackson Laboratories (Bar Harbor, Maine).
T260 35258-35504 Sentence denotes Transgenic DO11.10 mice, expressing a T cell receptor for OVA323–339 peptide in the context of H-2d, were backcrossed with mice expressing GATA-3, driven by the human CD2 locus control region (CD2-GATA3) [22], resulting in DO11.10xCD2-GATA3 mice.
T261 35505-35636 Sentence denotes Mice used for experiments were backcrossed on a BALB/c background for a minimum of eight generations and used at an age of 8–12 wk.
T262 35637-35780 Sentence denotes Mice were housed under specific pathogen-free conditions and all animal studies were performed according to institutional and state guidelines.
T263 35782-35808 Sentence denotes Isolation of CD4+ T cells.
T264 35809-35964 Sentence denotes CD4+ T cells were isolated from blood of healthy human volunteers using the anti-CD4 magnetic beads (Dynal, Hamburg, Germany) as previously described [60].
T265 35965-36035 Sentence denotes The purity of CD4+ T cells was initially tested by FACS and was ≥ 95%.
T266 36036-36143 Sentence denotes Monoclonality of T cell clones was confirmed by TCR-chain mapping and was identified to be Vbeta8 positive.
T267 36144-36197 Sentence denotes The clones were characterized by high IL-4 secretion.
T268 36199-36226 Sentence denotes Quantitative real-time PCR.
T269 36227-36655 Sentence denotes The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′.
T270 36656-36845 Sentence denotes The prepared cDNAs were amplified using SYBR-PCR mastermix (Biorad) according to the recommendations of the manufacturer in an ABI PRISM 7000 Sequence Detection System (Applied Biosystems).
T271 36846-36976 Sentence denotes Quantitative PCR of murine samples was performed with Brilliant SYBR Green QPCR master mix (Stratagene) and the following primers:
T272 36977-37223 Sentence denotes Ubiquitin C, 5′- AGG TCA AAC AGG AAG ACA GAC GTA-3′ and 5′-TCACACCCAAGAACAAGCACA-3′; Smad-7, 5′-GAA ACC GGG GGA ACG AAT TAT-3′ and 5′-CGC GAG TCT TCT CCT CCC A-3′; TGF-ß1, 5′-TGA CGT CAC TGG AGT TGT ACG G-3′ and 5′-GGT TCA TGT CAT GGA TGG TGC-3′.
T273 37224-37362 Sentence denotes Primer pairs were evaluated for integrity by analysis of the amplification plot, dissociation curves, and efficiency of PCR amplification.
T274 37363-37515 Sentence denotes PCR conditions were 10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C and 60 °C for 1 min using an 7300 real-time PCR system (Applied Biosystems).
T275 37516-37665 Sentence denotes PCR amplification of the housekeeping gene encoding ubiquitin C was performed during each run for each sample to allow normalization between samples.
T276 37666-37781 Sentence denotes Relative quantification and calculation of the range of confidence was performed using the comparative ΔΔCT method.
T277 37783-37813 Sentence denotes Inducible murine Treg culture.
T278 37814-37945 Sentence denotes Naive CD4+ T cells (CD4+, CD62L+, and CD25–) were isolated from pooled lymph nodes and spleens by FACS (FACS Aria, BD Biosciences).
T279 37946-38157 Sentence denotes 5 × 105 T cells were co-cultured with 2.5 × 104 bone marrow–derived dendritic cells [62] and 0.01 μg/ml OVA323–339 peptide (Ansynth) in the presence or absence of 20-ng/μl rhTGF-ß1 (Peprotech) in 48-well plates.
T280 38158-38288 Sentence denotes After 4 d, cells were harvested and analyzed for intracellular FOXP3 expression by FACS or gene expression by quantitative RT-PCR.
T281 38290-38322 Sentence denotes In vitro T cell differentiation.
T282 38323-38552 Sentence denotes CD4+ CD45RA+ magnetically-sorted (CD45RO depletion, MACS, according to the protocol of the manufacturer) cells were stimulated with immobilized plate-bound anti-CD3 (1 μg/ml, Okt3, IgG1) and anti-CD28 (2 μg/ml) in Th1 conditions:
T283 38553-38620 Sentence denotes 25 ng/ml IL-12, 5 μg/ml anti-IL-4 (R&D systems); in Th2 conditions:
T284 38621-38712 Sentence denotes 25 ng/ml IL-4, 5 μg/ml anti-IFN-γ, 5 μg/ml anti-IL-12 (R&D systems); or in Treg conditions:
T285 38713-38787 Sentence denotes 10 ng/ml TGF-β, 5 μg/ml anti-IFN-γ, 5 μg/ml anti-IL-12, 5 μg/ml anti-IL-4.
T286 38788-38859 Sentence denotes Proliferating cells were expanded in medium containing IL-2 (30 ng/ml).
T287 38861-38929 Sentence denotes Cloning of the FOXP3 promoter and construction of mutant constructs.
T288 38930-39047 Sentence denotes The FOXP3 promoter was cloned into the pGL3 basic vector (Promega Biotech) to generate the pGL3 FOXP3 −511/+176 [24].
T289 39048-39204 Sentence denotes Site-directed mutagenesis in the FOXP3 promoter region were introduced using the QuickChange kit (Stratagene), according to the manufacturer's instructions.
T290 39205-39333 Sentence denotes The following primer and its complementary strand were used: GTT TCT CAT GAG CCC TAT TAA GTC ATT CTT ACC TCT CAC CTC TGT GGT GA.
T291 39335-39374 Sentence denotes Transfections and reporter gene assays.
T292 39375-39452 Sentence denotes T cells were rested in serum-free AIM-V medium (Life Technologies) overnight.
T293 39453-39691 Sentence denotes 3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector.
T294 39692-39911 Sentence denotes After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions.
T295 39912-39971 Sentence denotes Data were normalized by the activity of renilla luciferase.
T296 39973-40006 Sentence denotes RNA isolation and cDNA synthesis.
T297 40007-40100 Sentence denotes RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol.
T298 40101-40280 Sentence denotes Reverse transcription of human samples was performed with TaqMan reverse-transcription reagents (Applied Biosystems) with random hexamers according to the manufacturer's protocol.
T299 40282-40307 Sentence denotes Recombinant TAT proteins.
T300 40308-40499 Sentence denotes The cDNAs encoding GATA3 protein or the truncated GATA3 (lacking the two zinc fingers) were cloned in frame into an expression vector along with the TAT sequence as previously described [63].
T301 40500-40625 Sentence denotes Proteins were expressed in BL21 Star (DE3)pLysS (Invitrogen) and lysates were purified by Ni2+-chelate column chromatography.
T302 40626-40745 Sentence denotes Both TAT-linked proteins were more than 95% pure, based on Coomassie blue staining of sodium disulfate acrylamide gels.
T303 40747-40770 Sentence denotes TAT-GATA3 transduction.
T304 40771-40919 Sentence denotes CD4+CD45RA+ cells were cultured in AIMV medium and transduced with 20 nM, 10 nM, or 500 nM of full-length or truncated GATA3 over the course of 4 h.
T305 40920-41024 Sentence denotes After 4 h, the cells were washed and activated with soluble anti-CD3 and anti-CD28 and TGF-β (10 ng/ml).
T306 41025-41085 Sentence denotes Each day, the TAT proteins were freshly added to the medium.
T307 41086-41152 Sentence denotes FOXP3 expression was measured after 5 d by intracellular staining.
T308 41154-41186 Sentence denotes Intracellular cytokine staining.
T309 41187-41282 Sentence denotes T cells were stimulated with 2 × 10−7 M PMA and 1 μg/ml of ionomycin (Sigma Chemicals) for 4 h.
T310 41283-41336 Sentence denotes The following mAb was used: anti-IL-4-PE (8D4–8, BD).
T311 41337-41435 Sentence denotes Matched isotype controls were used at the same protein concentration as the respective antibodies.
T312 41436-41576 Sentence denotes Four-color FACS was performed using an EPICS XL-MCL (Beckman Coulter) using the software Expo32 version for data acquisition and evaluation.
T313 41578-41593 Sentence denotes Flow cytometry.
T314 41594-41952 Sentence denotes For analysis of FOXP3 expression at the single-cell level, cells were first stained with the monoclonal antibody CD25 (Beckman Coulter), and after fixation and permeabilization, cells were incubated with PE-conjugated monoclonal antibody PCH101 (anti-human FOXP3; eBioscience) based on the manufacturer's recommendations and subjected to FACS (EPICS XL-MCL).
T315 41953-42278 Sentence denotes For cell surface marker staining, cells were incubated for 20 min at 4°C in staining buffer with the following antibodies: anti–CD152-PE (CTLA-4; BD), anti–PD-1 (eBiosciences), anti-GITR (R & D Systems), anti-CD69 (Beckman Coulter), anti-CD103 (DakoCytomation), anti-CD62L (Beckman Coulter), or anti-HLA-DR (Beckman Coulter).
T316 42279-42349 Sentence denotes The controls were FITC, PE, or ECD-conjugated mouse IgG1 or rat IgG2a.
T317 42350-42512 Sentence denotes For staining of mouse cells, the following mAbs from BD Biosciences were used following standard techniques as described above: anti-CD3, anti-CD4, and anti-CD25.
T318 42513-42621 Sentence denotes Anti-FcγRII/III antibody (2.4G2, ATCC) was included in all stainings to reduce nonspecific antibody binding.
T319 42622-42797 Sentence denotes To isolate naive murine CD4 T cells from murine DO11.10 or DO11.10xCD2-GATA3 T cells, cells were stained with anti-CD25-FITC, anti-CD62L-PE, and anti-CD4-APC prior to sorting.
T320 42798-42866 Sentence denotes Dead cells were excluded with 4′',6-Diamidino-2-phenylindole (DAPI).
T321 42867-43140 Sentence denotes To analyze murine Foxp3 expression in inducible Treg cultures, cells were stained intracellularly with anti-Foxp3-PE according to manufacturer's instruction, in conjunction with anti-CD4-APC and LIVE/DEAD fixable dead cell stain kit (Invitrogen) to discriminate live cells.
T322 43141-43243 Sentence denotes All monoclonal antibodies for murine cell stainings were purchased from eBioscience or BD Biosciences.
T323 43245-43262 Sentence denotes Western blotting.
T324 43263-43448 Sentence denotes For FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen).
T325 43449-43527 Sentence denotes The proteins were electroblotted onto a PVDF membrane (Amersham Life Science).
T326 43528-43702 Sentence denotes Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C.
T327 43703-43831 Sentence denotes The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji).
T328 43832-43900 Sentence denotes Membranes were incubated in stripping buffer and re-blocked for 1 h.
T329 43901-44151 Sentence denotes The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),
T330 44153-44204 Sentence denotes Administration of cytokines and antibodies in vivo.
T331 44205-44444 Sentence denotes Age- and gender-matched normal B6 mice received every other day intraperitoneal (ip) injections of PBS, 1.5 μg rmIL-4, 50 μg anti-IL-4 mAb (11B11 or MAB404), or a mixture of 1.5 μg rmIL-4 plus 50 μg anti-IL-4 mAb (11B11 or MAB404) for 7 d.
T332 44445-44551 Sentence denotes Thereafter, spleen and lymph node cells were analyzed by flow cytometry for CD3, CD4, and CD25 expression.
T333 44552-44682 Sentence denotes The anti-mouse IL-4 mAb MAB404 was obtained from R&D Systems, the second anti-mouse IL-4 mAb 11B11 was purchased from eBioscience.
T334 44684-44689 Sentence denotes ChIP.
T335 44690-44900 Sentence denotes ChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature.
T336 44901-45015 Sentence denotes The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin).
T337 45016-45174 Sentence denotes The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h.
T338 45175-45264 Sentence denotes Washing and elution buffers were used according to the protocol of Upstate Biotechnology.
T339 45265-45409 Sentence denotes Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction.
T340 45410-45463 Sentence denotes The amount of DNA was determined by conventional PCR.
T341 45464-45571 Sentence denotes The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers:
T342 45572-45633 Sentence denotes 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′.
T343 45634-45697 Sentence denotes The PCR products were visualized using an ethidium bromide gel.
T344 45699-45715 Sentence denotes Pull-down assay.
T345 45716-45784 Sentence denotes CD4+ T cells were stimulated with PMA and ionomycin for 2 h at 37°C.
T346 45785-45980 Sentence denotes The cells were pelleted, resuspended in buffer C (20 mM HEPES [pH 7.9], 420 mM NaCl, 1.5 mM MgCl2, 0.2 mM EDTA, 1 mM DTT, protease inhibitors [Sigma]. and 0.1% NP-40) and lysed on ice for 15 min.
T347 45981-46030 Sentence denotes Insoluble material was removed by centrifugation.
T348 46031-46109 Sentence denotes The supernatant was diluted 1:3 with buffer D (as buffer C, but without NaCl).
T349 46110-46284 Sentence denotes The lysates were incubated with 10 μg of poly(dI-dC) (Sigma) and 70 μl of streptavidin-agarose (Amersham Biosciences) carrying biotinylated oligonucleotides, for 3 h at 4 °C.
T350 46285-46494 Sentence denotes The beads were washed twice with buffer C:D (1:3) and resuspended in DTT-containing loading buffer (NuPAGE; Invitrogen), heated to 70 °C for 10 min, and the eluants on a NuPAGE 4–12% bis-tris gel (Invitrogen).
T351 46495-46636 Sentence denotes The proteins were electroblotted onto a PVDF membrane (Amersham Biosciences) and detected using an anti-GATA3 mAb (Santa Cruz Biotechnology).
T352 46637-46701 Sentence denotes Accumulated signals were analyzed using AIDA software (Raytest).
T353 46703-46725 Sentence denotes Supporting Information
T354 46726-46781 Sentence denotes Figure S1 Phenotype of In Vitro Differentiated T Cells
T355 46782-46987 Sentence denotes After two round of differentiation cultures, T cells were stimulated by plate-immobilized anti-CD3/CD28 and 3H-thymidine incorporation as measurement of proliferation was analyzed after 3 d of culture (A).
T356 46988-47100 Sentence denotes In parallel, T cells were analyzed for Treg relevant surface receptor expression as indicated on the x-axis (B).
T357 47101-47113 Sentence denotes (1.0 MB AI).
T358 47114-47150 Sentence denotes Click here for additional data file.
T359 47153-47227 Sentence denotes Figure S2 In Vivo Treatment of Mice with IL-4 Antibody–Cytokine Complexes
T360 47228-47456 Sentence denotes B6 mice were given every other day ip injections of phosphate-buffered saline (PBS), recombinant mouse IL-4 (rmIL-4), anti-IL-4 mAb (anti-IL-4 mAb, 11B11, or MAB404), or a mixture of rmIL-4 plus anti-IL-4 mAbs (11B11 or MAB404).
T361 47457-47537 Sentence denotes Mice were analyzed on day 7 by flow cytometry for CD3, CD4, and CD25 expression.
T362 47538-47606 Sentence denotes Shown is CD25 versus CD4 expression in CD3+ CD4+ spleen cells (A–F).
T363 47607-47664 Sentence denotes Numbers indicate percentages of CD4+ CD25high CD3+ cells.
T364 47665-47762 Sentence denotes Total cell counts (G) of CD4+ CD25high cells in spleen from mice in (A–F) are shown as mean ± SD.
T365 47763-47824 Sentence denotes The data are representative of three independent experiments.
T366 47825-47837 Sentence denotes (369 KB AI).
T367 47838-47874 Sentence denotes Click here for additional data file.
T368 47877-47954 Sentence denotes Figure S3 Effect of IL-4 on Already Existing Natural or Inducible Treg Cells
T369 47955-48159 Sentence denotes (A) CD4+CD25high nTreg cells were FACS-sorted and activated with plate-bound anti-CD3/CD28 plus IL-2 during 3 d and in the presence or absence of IL-4 (100 ng/ml) and harvested for real-time PCR analysis.
T370 48160-48235 Sentence denotes The results shown represent the mean ± SD of three independent experiments.
T371 48236-48274 Sentence denotes (B) iTreg cells were induced in vitro.
T372 48275-48523 Sentence denotes FOXP3 espression was assessed by real-time PCR analysis in resting cells, in cells re-stimulated with plate-bound anti-CD3/CD28, with or without TGF-β, plus IL-2 during 3 d and in the presence (black bar) or absence (white bar) of IL-4 (100 ng/ml).
T373 48524-48629 Sentence denotes (C) Activation dramatically increases CD4+CD25+ Treg cells suppressive capacity of CD4+CD25+ nTreg cells.
T374 48630-48744 Sentence denotes CD4+CD25+ nTreg cells were preactivated during 2 d in the presence or absence of an increasing IL-4 concentration.
T375 48745-48830 Sentence denotes After vigorous washing, their suppressive capacity on responder CD4+CD25− was tested.
T376 48831-48923 Sentence denotes IL-4 pretreatment did not affect the suppressive capacity of FACS-sorted CD4+CD25high cells.
T377 48924-49015 Sentence denotes 1 × 104 CD4+CD25+ nTreg cells were added to 5 × 104 CD4+CD25– and 5 × 104 irradiated PBMCs.
T378 49016-49080 Sentence denotes The results are representative of three independent experiments.
T379 49081-49093 Sentence denotes (269 KB AI).
T380 49094-49130 Sentence denotes Click here for additional data file.
T381 49133-49213 Sentence denotes Figure S4 Schematic Structure of the FOXP3 Gene and Location of the GATA3 Sites
T382 49214-49332 Sentence denotes The scheme shows the location of the 11 exons spaced by a large intron (6000 bp) from the 5′untranslated region (UTR).
T383 49333-49440 Sentence denotes Human, murine, and rat sequences are aligned and transcription start site (TSS) is indicated with an arrow.
T384 49441-49453 Sentence denotes (529 KB AI).
T385 49454-49490 Sentence denotes Click here for additional data file.

ICD10

Id Subject Object Predicate Lexical cue
T630 1485-1492 http://purl.bioontology.org/ontology/ICD10/T78.4 denotes allergy
T16682 35127-35134 http://purl.bioontology.org/ontology/ICD10/T78.4 denotes allergy
T16683 35139-35145 http://purl.bioontology.org/ontology/ICD10/J45.9 denotes asthma
T16684 35139-35145 http://purl.bioontology.org/ontology/ICD10/J45 denotes asthma

events-check-again

Id Subject Object Predicate Lexical cue Negation Speculation
T768 944-978 Protein denotes transforming growth factor (TGF)-β
T769 979-987 Positive_regulation denotes mediated
T770 988-993 Protein denotes FOXP3
T771 994-1003 Positive_regulation denotes induction
T772 1024-1032 Positive_regulation denotes mediated
T773 1043-1050 Binding denotes binding
T774 1054-1059 Protein denotes GATA3
T775 1067-1072 Protein denotes FOXP3
T776 1073-1081 Entity denotes promoter
T777 1245-1249 Protein denotes IL-4
T778 1344-1350 Regulation denotes target
T779 1344-1350 Regulation denotes target
T780 1351-1355 Protein denotes IL-4
T781 1359-1364 Protein denotes GATA3
T2517 3496-3509 Protein denotes interleukin 4
T2518 3511-3515 Protein denotes IL-4
T2519 3517-3524 Positive_regulation denotes induced
T2520 3517-3524 Positive_regulation denotes induced
T2521 3525-3530 Protein denotes STAT6
T2522 3535-3540 Protein denotes GATA3
T2523 3620-3625 Protein denotes GATA3
T2524 3700-3709 Positive_regulation denotes induction
T2525 3700-3709 Positive_regulation denotes induction
T2526 3700-3709 Positive_regulation denotes induction
T2527 3713-3717 Protein denotes IL-4
T2528 3719-3723 Protein denotes IL-5
T2529 3728-3733 Protein denotes IL-13
T2530 3848-3853 Protein denotes T-bet
T2531 3863-3872 Regulation denotes modulates
T2532 3873-3878 Protein denotes GATA3
T2533 4077-4082 Protein denotes GATA3
T2534 4177-4181 Protein denotes IL-4
T2535 4251-4293 Negative_regulation denotes Overexpression of a dominant negative form
T2536 4297-4302 Protein denotes GATA3
T2537 4344-4349 Protein denotes GATA3
T2538 4350-4358 Negative_regulation denotes blockade
T2539 4756-4763 Gene_expression denotes express
T2540 4798-4803 Protein denotes FOXP3
T2541 4823-4830 Gene_expression denotes express
T2542 4831-4835 Protein denotes CD25
T2543 5098-5105 Gene_expression denotes express
T2544 5106-5111 Protein denotes FOXP3
T2545 5227-5233 Positive_regulation denotes induce
T2546 5234-5239 Protein denotes FOXP3
T2547 5240-5250 Gene_expression denotes expression
T2548 5356-5361 Protein denotes TGF-β
T2549 5480-5487 Negative_regulation denotes lacking
T2550 5492-5500 Protein denotes TGF-βRII
T2551 5649-5654 Protein denotes TGF-β
T2552 5833-5838 Protein denotes GATA3
T2553 5843-5848 Protein denotes FOXP3
T2554 5901-5906 Protein denotes T-bet
T2555 5911-5916 Protein denotes GATA3
T2556 5977-5982 Protein denotes GATA3
T2557 5983-5991 Negative_regulation denotes inhibits
T2558 5992-5997 Protein denotes FOXP3
T2559 5998-6007 Positive_regulation denotes induction
T2560 6017-6021 Protein denotes IL-4
T2561 6022-6028 Negative_regulation denotes limits
T2562 6029-6034 Protein denotes FOXP3
T2563 6035-6045 Gene_expression denotes expression
T2564 6051-6056 Protein denotes GATA3
T2565 6057-6069 Positive_regulation denotes mediated way
T2566 6116-6121 Protein denotes GATA3
T2567 6131-6136 Binding denotes binds
T2568 6144-6149 Protein denotes FOXP3
T2569 6150-6158 Entity denotes promoter
T2570 6171-6179 Negative_regulation denotes prevents
T2571 6184-6193 Positive_regulation denotes induction
T4838 6284-6289 Protein denotes FOXP3
T4839 6290-6299 Positive_regulation denotes Induction
T4840 6337-6342 Protein denotes FOXP3
T4841 6343-6353 Gene_expression denotes expression
T4842 6361-6368 Positive_regulation denotes induced
T4843 6485-6488 Protein denotes CD4
T4844 6524-6531 Gene_expression denotes express
T4845 6532-6537 Protein denotes FOXP3
T4846 6562-6567 Protein denotes FOXP3
T4847 6575-6584 Gene_expression denotes expressed true
T4848 6612-6622 Gene_expression denotes expression
T4849 6626-6636 Regulation denotes restricted true
T4850 6660-6665 Protein denotes FOXP3
T4851 6666-6681 Transcription denotes mRNA expression true
T4852 6731-6735 Protein denotes CD25
T4853 6745-6748 Protein denotes CD4
T4854 6757-6763 Protein denotes CD45RA
T4855 6774-6780 Protein denotes CD45RO
T4856 6923-6926 Protein denotes CD4
T4857 6927-6931 Protein denotes CD25
T4858 6934-6940 Protein denotes CD45RA
T4859 6943-6949 Protein denotes CD45RO
T4860 6956-6959 Protein denotes CD4
T4861 6960-6966 Protein denotes CD45RO
T4862 6967-6971 Protein denotes CD25
T4863 7000-7006 Positive_regulation denotes induce
T4864 7007-7012 Protein denotes FOXP3
T4865 7068-7073 Protein denotes TGF-β
T4866 7185-7193 Positive_regulation denotes increase true
T4867 7194-7199 Protein denotes FOXP3
T4868 7200-7210 Gene_expression denotes expression
T4869 7270-7281 Positive_regulation denotes up-regulate
T4870 7282-7287 Protein denotes FOXP3
T4871 7980-7987 Gene_expression denotes produce
T4872 7988-7992 Protein denotes IL-4
T4873 8034-8039 Protein denotes TGF-β
T4874 8059-8066 Negative_regulation denotes prevent true
T4875 8067-8072 Protein denotes FOXP3
T4876 8073-8082 Positive_regulation denotes induction
T4877 8097-8111 Negative_regulation denotes neutralization
T4878 8115-8119 Protein denotes IL-4
T4879 8136-8140 Protein denotes IL-4
T4880 8158-8164 Positive_regulation denotes rescue true
T4881 8165-8170 Protein denotes FOXP3
T4882 8171-8181 Gene_expression denotes expression
T4883 8300-8307 Gene_expression denotes express
T4884 8308-8313 Protein denotes FOXP3
T4885 8331-8340 Negative_regulation denotes inability
T4886 8357-8364 Gene_expression denotes express
T4887 8365-8370 Protein denotes FOXP3
T4888 8443-8447 Negative_regulation denotes lack
T4889 8448-8453 Protein denotes FOXP3
T4890 8454-8464 Gene_expression denotes expression
T4891 8495-8504 Gene_expression denotes expressed
T4892 8505-8510 Protein denotes FOXP3
T4893 8586-8593 Gene_expression denotes express
T4894 8594-8599 Protein denotes FOXP3
T4895 8609-8612 Positive_regulation denotes low true
T4896 8613-8617 Protein denotes CD25
T4897 8626-8636 Gene_expression denotes expression
T4898 8659-8664 Protein denotes TGF-β
T4899 8681-8689 Positive_regulation denotes increase true
T4900 8694-8699 Protein denotes FOXP3
T4901 8700-8710 Gene_expression denotes expression
T4902 8748-8755 Positive_regulation denotes induced
T4903 8756-8761 Protein denotes FOXP3
T4904 8762-8772 Gene_expression denotes expression
T4905 8825-8830 Protein denotes CRTH2
T4906 8877-8881 Protein denotes IL-4
T4907 8882-8891 Gene_expression denotes secretion
T4908 8899-8903 Protein denotes IL-4
T4909 8904-8913 Gene_expression denotes producing
T4910 8943-8950 Negative_regulation denotes lacking
T4911 8951-8956 Protein denotes FOXP3
T4912 8957-8967 Gene_expression denotes expression
T4913 8993-8998 Protein denotes TGF-β
T4914 9008-9013 Protein denotes FOXP3
T4915 9014-9023 Positive_regulation denotes induction
T4916 9024-9030 Negative_regulation denotes failed
T4917 9100-9104 Protein denotes IL-4
T4918 9134-9144 Gene_expression denotes expression true
T4919 9134-9144 Gene_expression denotes expression true
T4920 9148-9152 Protein denotes IL-4
T4921 9157-9162 Protein denotes FOXP3
T4922 9183-9186 Protein denotes CD4
T4923 9256-9260 Protein denotes IL-4
T4924 9261-9271 Gene_expression denotes expressing
T4925 9303-9309 Protein denotes CD45RO
T4926 9310-9314 Protein denotes CD25
T4927 9337-9347 Gene_expression denotes co-express true
T4928 9348-9353 Protein denotes FOXP3
T4929 9392-9398 Protein denotes CD45RO
T4930 9399-9403 Protein denotes CD25
T4931 9422-9431 Gene_expression denotes expressed
T4932 9432-9437 Protein denotes FOXP3
T4933 9445-9452 Negative_regulation denotes lacking
T4934 9453-9457 Protein denotes IL-4
T4935 9528-9532 Protein denotes IL-4
T4936 9567-9572 Protein denotes FOXP3
T4937 9609-9613 Protein denotes IL-4
T4938 9642-9647 Protein denotes FOXP3
T4939 9673-9679 Protein denotes CD45RO
T4940 9680-9684 Protein denotes CD25
T4941 9687-9693 Protein denotes CD45RO
T4942 9694-9698 Protein denotes CD25
T4943 9705-9711 Protein denotes CD45RO
T4944 9712-9716 Protein denotes CD25
T4945 9786-9791 Protein denotes CRTH2
T4946 9812-9821 Gene_expression denotes expressed
T4947 9822-9827 Protein denotes FOXP3
T4948 9866-9870 Protein denotes IL-4
T4949 9871-9880 Gene_expression denotes secretion
T4950 9941-9946 Protein denotes FOXP3
T4951 9947-9957 Gene_expression denotes expressing
T4952 10055-10060 Protein denotes FOXP3
T4953 10069-10078 Gene_expression denotes expressed true
T4954 10104-10113 Positive_regulation denotes inducible true
T6106 11403-11408 Protein denotes FOXP3
T6107 11413-11418 Protein denotes GATA3
T6108 11510-11516 Positive_regulation denotes induce
T6109 11517-11522 Protein denotes FOXP3
T6110 11523-11533 Gene_expression denotes expression
T6111 11651-11661 Gene_expression denotes expression true
T6112 11651-11661 Gene_expression denotes expression true
T6113 11665-11670 Protein denotes FOXP3
T6114 11675-11680 Protein denotes GATA3
T6115 11717-11720 Protein denotes CD4
T6116 11753-11757 Protein denotes IL-4
T6117 11764-11769 Protein denotes IFN-γ
T6118 11780-11785 Protein denotes IL-12
T6119 11864-11869 Protein denotes FOXP3
T6120 11874-11879 Protein denotes GATA3
T6121 11897-11907 Gene_expression denotes expression
T6122 11897-11907 Gene_expression denotes expression
T6123 12039-12044 Protein denotes FOXP3
T6124 12045-12060 Transcription denotes mRNA expression
T6125 12061-12070 Positive_regulation denotes increased
T6126 12127-12132 Protein denotes GATA3
T6127 12137-12142 Protein denotes FOXP3
T6128 12174-12184 Gene_expression denotes expression
T6129 12174-12184 Gene_expression denotes expression
T6130 12357-12367 Gene_expression denotes expressing
T6131 12368-12372 Positive_regulation denotes more
T6132 12373-12378 Protein denotes FOXP3
T6133 12403-12412 Gene_expression denotes expressed
T6134 12413-12416 Positive_regulation denotes low true
T6135 12427-12432 Protein denotes GATA3
T6136 12703-12706 Protein denotes CD3
T6137 12736-12741 Protein denotes CD103
T6138 12743-12749 Protein denotes CTLA-4
T6139 12751-12755 Protein denotes GITR
T6140 12761-12765 Protein denotes PD-1
T6141 12774-12784 Gene_expression denotes expression
T6142 12774-12784 Gene_expression denotes expression
T6143 12774-12784 Gene_expression denotes expression
T6144 12774-12784 Gene_expression denotes expression
T6145 12894-12899 Protein denotes GATA3
T6146 12904-12909 Protein denotes FOXP3
T6147 12910-12920 Gene_expression denotes expression
T6148 12910-12920 Gene_expression denotes expression
T6149 12972-12980 Negative_regulation denotes resolved
T6150 12972-12980 Negative_regulation denotes resolved
T6151 13079-13083 Negative_regulation denotes lost
T6152 13102-13109 Gene_expression denotes express
T6153 13110-13115 Protein denotes FOXP3
T7945 13785-13789 Protein denotes IL-4
T7946 13799-13804 Protein denotes TGF-β
T7947 13831-13835 Protein denotes IL-4
T7948 13956-13960 Protein denotes IL-4
T7949 13972-13979 Negative_regulation denotes inhibit true
T7950 13980-13985 Protein denotes TGF-β
T7951 13986-13995 Positive_regulation denotes induction
T7952 13999-14004 Protein denotes FOXP3
T7953 14048-14051 Protein denotes CD4
T7954 14052-14058 Protein denotes CD45RA
T7955 14105-14108 Protein denotes CD3
T7956 14109-14113 Protein denotes CD28
T7957 14133-14138 Protein denotes TGF-β
T7958 14146-14150 Protein denotes IL-4
T7959 14176-14180 Protein denotes IL-4
T7960 14193-14202 Negative_regulation denotes repressed
T7961 14207-14212 Protein denotes TGF-β
T7962 14222-14231 Positive_regulation denotes induction
T7963 14235-14240 Protein denotes FOXP3
T7964 14241-14251 Gene_expression denotes expression
T7965 14327-14332 Protein denotes GATA3
T7966 14338-14345 Negative_regulation denotes induced true
T7967 14358-14365 Negative_regulation denotes absence
T7968 14369-14373 Protein denotes IL-4
T7969 14441-14445 Protein denotes IL-4
T7970 14469-14475 Positive_regulation denotes induce
T7971 14476-14481 Protein denotes FOXP3
T7972 14482-14492 Gene_expression denotes expression
T7973 14503-14508 Protein denotes GATA3
T7974 14518-14525 Positive_regulation denotes induced
T7975 14518-14525 Positive_regulation denotes induced
T7976 14545-14550 Protein denotes TGF-β
T7977 14559-14563 Protein denotes IL-4
T7978 14602-14606 Protein denotes IL-4
T7979 14616-14626 Negative_regulation denotes prevention
T7980 14630-14635 Protein denotes FOXP3
T7981 14636-14646 Gene_expression denotes expression
T7982 14655-14661 Positive_regulation denotes caused true
T7983 14718-14733 Phosphorylation denotes phosphorylation
T7984 14718-14733 Phosphorylation denotes phosphorylation
T7985 14737-14742 Protein denotes SMAD2
T7986 14746-14751 Protein denotes STAT6
T7987 14760-14768 Regulation denotes affected true
T7988 14760-14768 Regulation denotes affected true
T7989 14760-14768 Regulation denotes affected true
T7990 14760-14768 Regulation denotes affected true
T7991 14788-14792 Protein denotes IL-4
T7992 14800-14805 Protein denotes TGF-β
T7993 14831-14835 Protein denotes IL-4
T7994 14847-14852 Protein denotes TGF-β
T7995 14915-14933 Positive_regulation denotes Increasing amounts
T7996 14937-14941 Protein denotes IL-4
T7997 14942-14950 Positive_regulation denotes increase
T7998 14965-14970 Protein denotes GATA3
T7999 14980-14985 Protein denotes FOXP3
T8000 14986-14995 Negative_regulation denotes decreased
T8001 15079-15083 Protein denotes IL-4
T8002 15258-15265 Gene_expression denotes express
T8003 15274-15278 Protein denotes CD25
T8004 15319-15323 Protein denotes IL-4
T8005 15325-15331 Protein denotes rmIL-4
T8006 15343-15347 Protein denotes IL-4
T8007 15500-15503 Protein denotes CD4
T8008 15504-15508 Protein denotes CD25
T8009 15514-15519 Protein denotes FOXP3
T8010 15646-15652 Protein denotes rmIL-4
T8011 15663-15667 Protein denotes IL-4
T8012 15703-15706 Protein denotes CD4
T8013 15707-15711 Protein denotes CD25
T8014 15736-15741 Protein denotes Foxp3
T8015 15863-15866 Protein denotes CD4
T8016 15867-15871 Protein denotes CD25
T8017 15903-15906 Protein denotes CD4
T8018 15907-15911 Protein denotes CD25
T8019 15937-15941 Protein denotes IL-4
T8020 16067-16071 Protein denotes IL-4
T8021 16149-16153 Protein denotes CD25
T8022 16180-16184 Protein denotes IL-4
T8023 16198-16203 Protein denotes FOXP3
T8024 16204-16214 Gene_expression denotes expression
T8025 16219-16239 Negative_regulation denotes suppressive capacity true
T8026 16273-16277 Protein denotes IL-4
T8027 16288-16295 Negative_regulation denotes inhibit true
T8028 16296-16301 Protein denotes FOXP3
T8029 16302-16312 Gene_expression denotes expression
T8030 16364-16371 Regulation denotes altered true
T8031 16429-16437 Negative_regulation denotes decrease true
T8032 16438-16443 Protein denotes FOXP3
T8033 16444-16454 Gene_expression denotes expression
T8034 16460-16464 Protein denotes IL-4
T9435 17557-17562 Protein denotes TGF-β
T9436 17598-17603 Protein denotes TGF-β
T9437 17604-17611 Negative_regulation denotes reduced
T9438 17604-17611 Negative_regulation denotes reduced
T9439 17604-17611 Negative_regulation denotes reduced
T9440 17612-17616 Protein denotes CD25
T9441 17618-17622 Protein denotes IL-4
T9442 17623-17633 Gene_expression denotes expression
T9443 17623-17633 Gene_expression denotes expression
T9444 17639-17643 Protein denotes CD25
T9445 17644-17654 Gene_expression denotes expression
T9446 17668-17672 Protein denotes IL-4
T9447 17687-17696 Negative_regulation denotes inhibited
T9448 17697-17702 Protein denotes TGF-β
T9449 17712-17721 Positive_regulation denotes induction
T9450 17725-17730 Protein denotes FOXP3
T9451 17762-17767 Protein denotes FOXP3
T9452 17834-17838 Protein denotes IL-4
T9453 17910-17915 Protein denotes FOXP3
T9454 17968-17973 Protein denotes FOXP3
T9455 18061-18065 Protein denotes IL-4
T9456 18112-18117 Protein denotes FOXP3
T9457 18132-18137 Protein denotes FOXP3
T9458 18234-18239 Protein denotes TGF-β
T9459 18249-18258 Positive_regulation denotes induction
T9460 18262-18267 Protein denotes FOXP3
T9461 18268-18278 Gene_expression denotes expression
T9462 18287-18293 Positive_regulation denotes caused true
T9463 18313-18317 Protein denotes CD25
T9464 18318-18323 Protein denotes FOXP3
T9465 18355-18360 Protein denotes FOXP3
T9466 18399-18402 Protein denotes CD4
T9467 18403-18409 Protein denotes CD45RA
T9468 18448-18453 Protein denotes FOXP3
T9469 18508-18512 Protein denotes CD25
T9470 18517-18531 Negative_regulation denotes down-regulated
T9471 18535-18540 Protein denotes TGF-β
T9472 18605-18615 Positive_regulation denotes pronounced
T9473 18639-18644 Protein denotes TGF-β
T9474 18649-18653 Protein denotes IL-4
T9475 19296-19300 Protein denotes IL-4
T9476 19353-19358 Protein denotes FOXP3
T9477 19419-19423 Protein denotes IL-4
T9478 19424-19433 Gene_expression denotes producing
T9479 19448-19453 Protein denotes FOXP3
T9480 19454-19464 Gene_expression denotes expressing
T9481 19509-19514 Protein denotes FOXP3
T9482 19597-19602 Protein denotes GATA3
T9483 19607-19612 Protein denotes FOXP3
T9484 19613-19623 Gene_expression denotes expression true
T9485 19613-19623 Gene_expression denotes expression true
T9486 19676-19680 Protein denotes IL-4
T9487 19685-19690 Protein denotes TGF-β
T9488 19691-19703 Positive_regulation denotes demonstrated
T9489 19691-19703 Positive_regulation denotes demonstrated
T9490 19691-19703 Positive_regulation denotes demonstrated
T9491 19691-19703 Positive_regulation denotes demonstrated
T9492 19709-19714 Protein denotes GATA3
T9493 19719-19724 Protein denotes FOXP3
T9494 19794-19804 Gene_expression denotes co-express
T9495 19794-19804 Gene_expression denotes co-express
T9496 19950-19955 Protein denotes GATA3
T9497 19999-20009 Negative_regulation denotes repressing true
T9498 20010-20015 Protein denotes FOXP3
T9499 20043-20047 Protein denotes IL-4
T9500 20048-20077 Negative_regulation denotes acts in vitro as an inhibitor
T9501 20081-20086 Protein denotes FOXP3
T9502 20087-20097 Gene_expression denotes expression
T9503 20124-20129 Protein denotes TGF-β
T9504 20150-20156 Positive_regulation denotes acting true
T9505 20214-20219 Protein denotes GATA3
T9506 20220-20229 Regulation denotes dependent true
T11187 20242-20247 Protein denotes GATA3
T11188 20253-20271 Negative_regulation denotes Negative Regulator
T11189 20275-20280 Protein denotes FOXP3
T11190 20281-20291 Gene_expression denotes Expression
T11191 20292-20297 Protein denotes FOXP3
T11192 20298-20308 Gene_expression denotes expression
T11193 20309-20318 Negative_regulation denotes decreased
T11194 20324-20329 Protein denotes GATA3
T11195 20330-20340 Gene_expression denotes expression
T11196 20344-20348 Positive_regulation denotes high
T11197 20389-20393 Regulation denotes role true
T11198 20398-20403 Protein denotes GATA3
T11199 20407-20417 Negative_regulation denotes repressing
T11200 20418-20423 Protein denotes FOXP3
T11201 20433-20438 Protein denotes GATA3
T11202 20488-20493 Protein denotes GATA3
T11203 20572-20582 Negative_regulation denotes inhibiting
T11204 20583-20588 Protein denotes STAT4
T11205 20589-20599 Gene_expression denotes expression
T11206 20622-20627 Protein denotes GATA3
T11207 20692-20697 Protein denotes GATA3
T11208 20711-20718 Negative_regulation denotes inhibit true
T11209 20719-20724 Protein denotes FOXP3
T11210 20725-20734 Positive_regulation denotes induction
T11211 20750-20755 Protein denotes GATA3
T11212 20771-20776 Protein denotes GATA3
T11213 20777-20784 Negative_regulation denotes lacking
T11214 20789-20807 Entity denotes DNA-binding domain
T11215 20825-20828 Protein denotes CD4
T11216 20829-20835 Protein denotes CD45RA
T11217 20853-20893 Protein denotes TAT-fused, recombinantly expressed GATA3
T11218 20958-20961 Protein denotes CD3
T11219 20962-20966 Protein denotes CD28
T11220 20997-21002 Protein denotes TGF-β
T11221 21004-21013 Protein denotes TAT-GATA3
T11222 21096-21099 Protein denotes CD4
T11223 21135-21144 Protein denotes TAT-GATA3
T11224 21145-21152 Negative_regulation denotes reduced
T11225 21153-21158 Protein denotes FOXP3
T11226 21159-21169 Gene_expression denotes expression
T11227 21208-21226 Entity denotes DNA-binding domain
T11228 21227-21236 Negative_regulation denotes truncated
T11229 21255-21260 Protein denotes GATA3
T11230 21270-21276 Regulation denotes affect true
T11231 21277-21282 Protein denotes FOXP3
T11232 21283-21293 Gene_expression denotes expression
T11233 21386-21403 Negative_regulation denotes inhibitory effect true
T11234 21407-21412 Protein denotes GATA3
T11235 21416-21421 Protein denotes FOXP3
T11236 21465-21479 Positive_regulation denotes overexpressing
T11237 21480-21485 Protein denotes GATA3
T11238 21511-21514 Protein denotes CD2
T11239 21545-21548 Protein denotes CD2
T11240 21549-21554 Protein denotes GATA3
T11241 21587-21590 Protein denotes CD4
T11242 21628-21631 Protein denotes CD2
T11243 21632-21637 Protein denotes GATA3
T11244 21793-21799 Regulation denotes effect true
T11245 21803-21808 Protein denotes GATA3
T11246 21819-21822 Protein denotes CD4
T11247 21823-21828 Protein denotes CD62L
T11248 21829-21833 Protein denotes CD25
T11249 21905-21910 Protein denotes TGF-β
T11250 21916-21921 Protein denotes Foxp3
T11251 21922-21932 Gene_expression denotes expression true
T11252 21967-21970 Protein denotes CD4
T11253 21971-21975 Protein denotes CD25
T11254 21988-21993 Protein denotes Foxp3
T11255 22049-22054 Protein denotes TGF-β
T11256 22068-22080 Positive_regulation denotes up-regulated
T11257 22081-22086 Protein denotes Foxp3
T11258 22155-22158 Protein denotes CD2
T11259 22159-22164 Protein denotes GATA3
T11260 22198-22205 Negative_regulation denotes reduced
T11261 22206-22211 Protein denotes Foxp3
T11262 22212-22222 Gene_expression denotes expression
T11263 22228-22237 Positive_regulation denotes activated
T11264 22243-22248 Protein denotes TGF-β
T11265 22279-22287 Gene_expression denotes produced
T11266 22307-22312 Protein denotes TGF-β
T11267 22327-22332 Protein denotes Smad7
T11268 22345-22354 Gene_expression denotes expressed
T11269 22423-22428 Protein denotes TGF-β
T11270 23382-23386 Protein denotes IL-4
T11271 23387-23394 Positive_regulation denotes induced
T11272 23395-23400 Protein denotes GATA3
T12887 23457-23462 Protein denotes GATA3
T12888 23463-23472 Negative_regulation denotes Represses
T12889 23477-23482 Protein denotes FOXP3
T12890 23483-23491 Entity denotes Promoter
T12891 23534-23539 Protein denotes GATA3
T12892 23549-23559 Negative_regulation denotes repression true
T12893 23569-23574 Protein denotes FOXP3
T12894 23586-23591 Protein denotes FOXP3
T12895 23648-23653 Protein denotes GATA3
T12896 23674-23691 Entity denotes GATA-binding site
T12897 23856-23878 Regulation denotes play an important role true
T12898 23882-23887 Protein denotes FOXP3
T12899 23888-23898 Regulation denotes regulation
T12900 23958-23963 Protein denotes FOXP3
T12901 24018-24021 Protein denotes CD4
T12902 24126-24133 Gene_expression denotes express
T12903 24134-24139 Protein denotes GATA3
T12904 24166-24171 Protein denotes FOXP3
T12905 24172-24180 Entity denotes promoter
T12906 24226-24231 Protein denotes GATA3
T12907 24232-24242 Gene_expression denotes expressing
T12908 24331-24337 Positive_regulation denotes active
T12909 24345-24348 Protein denotes CD4
T12910 24362-24369 Gene_expression denotes express true
T12911 24388-24393 Protein denotes GATA3
T12912 24407-24421 Positive_regulation denotes Overexpression
T12913 24425-24430 Protein denotes GATA3
T12914 24486-24491 Protein denotes FOXP3
T12915 24635-24643 Negative_regulation denotes deleting
T12916 24648-24666 Entity denotes GATA3-binding site
T12917 24732-24735 Protein denotes CD4
T12918 24736-24742 Protein denotes CD45RO
T12919 24798-24803 Protein denotes GATA3
T12920 24806-24809 Protein denotes CD4
T12921 24810-24816 Protein denotes CD45RA
T12922 24839-24848 Negative_regulation denotes repressor
T12923 24861-24866 Protein denotes GATA3
T12924 24874-24879 Protein denotes FOXP3
T12925 24880-24888 Entity denotes promoter
T12926 24914-24919 Protein denotes GATA3
T12927 24920-24925 Binding denotes binds
T12928 24942-24947 Protein denotes FOXP3
T12929 24948-24956 Entity denotes promoter
T12930 25037-25042 Protein denotes GATA3
T12931 25168-25173 Protein denotes FOXP3
T12932 25268-25273 Protein denotes GATA3
T12933 25274-25281 Binding denotes binding true
T12934 25323-25328 Protein denotes GATA3
T12935 25329-25339 Gene_expression denotes expressing
T12936 25414-25428 Gene_expression denotes overexpressing
T12937 25429-25434 Protein denotes GATA3
T12938 25456-25461 Protein denotes GATA3
T12939 25462-25469 Binding denotes binding
T12940 25535-25540 Protein denotes GATA3
T12941 25541-25546 Binding denotes binds
T12942 25563-25568 Protein denotes FOXP3
T12943 25569-25577 Entity denotes promoter
T12944 25690-25695 Protein denotes GATA3
T12945 25721-25726 Protein denotes GATA3
T12946 25727-25732 Binding denotes binds
T12947 25740-25745 Protein denotes FOXP3
T12948 25746-25761 Entity denotes promoter region
T12949 25859-25864 Protein denotes GATA3
T12950 25865-25872 Binding denotes binding
T12951 25880-25885 Protein denotes FOXP3
T12952 25898-25908 Negative_regulation denotes repressing
T12953 25909-25914 Protein denotes FOXP3
T12954 25915-25925 Gene_expression denotes expression
T17339 27656-27661 Protein denotes FOXP3
T17340 27662-27671 Positive_regulation denotes induction
T17341 27715-27724 Negative_regulation denotes inhibited
T17342 27728-27733 Protein denotes GATA3
T17343 27882-27887 Protein denotes FOXP3
T17344 27948-27951 Protein denotes CD4
T17345 27952-27956 Protein denotes CD25
T17346 27977-27988 Positive_regulation denotes up-regulate
T17347 27989-27994 Protein denotes FOXP3
T17348 28100-28105 Protein denotes FOXP3
T17349 28106-28115 Positive_regulation denotes induction
T17350 28246-28257 Positive_regulation denotes up-regulate
T17351 28258-28263 Protein denotes FOXP3
T17352 28282-28287 Protein denotes TGF-β
T17353 28338-28343 Protein denotes FOXP3
T17354 28481-28486 Protein denotes T-bet
T17355 28491-28496 Protein denotes GATA3
T17356 28546-28556 Gene_expression denotes expression
T17357 28546-28556 Gene_expression denotes expression
T17358 28625-28630 Protein denotes FOXP3
T17359 28665-28678 Gene_expression denotes co-expression
T17360 28665-28678 Gene_expression denotes co-expression
T17361 28712-28722 Gene_expression denotes expression
T17362 28712-28722 Gene_expression denotes expression
T17363 28783-28788 Protein denotes TGF-β
T17364 28835-28840 Protein denotes FOXP3
T17365 28866-28876 Gene_expression denotes expression
T17366 28866-28876 Gene_expression denotes expression
T17367 28880-28885 Protein denotes GATA3
T17368 28890-28895 Protein denotes T-bet
T17369 28896-28899 Negative_regulation denotes low true
T17370 28896-28899 Negative_regulation denotes low true
T17371 28959-28966 Negative_regulation denotes absence
T17372 28959-28966 Negative_regulation denotes absence
T17373 28959-28966 Negative_regulation denotes absence
T17374 29005-29009 Protein denotes IL-4
T17375 29011-29016 Protein denotes IL-12
T17376 29021-29026 Protein denotes TGF-β
T17377 29051-29056 Protein denotes FOXP3
T17378 29057-29067 Gene_expression denotes expression
T17379 29107-29112 Protein denotes FOXP3
T17380 29113-29123 Gene_expression denotes expressing
T17381 29135-29140 Protein denotes GATA3
T17382 29145-29150 Protein denotes T-bet
T17383 29156-29168 Positive_regulation denotes up-regulated
T17384 29156-29168 Positive_regulation denotes up-regulated
T17385 29243-29248 Protein denotes FOXP3
T17386 29249-29258 Positive_regulation denotes promoting
T17387 29296-29302 Protein denotes CTLA-4
T17388 29347-29357 Negative_regulation denotes inhibitors
T17389 29361-29366 Protein denotes GATA3
T17390 29367-29377 Gene_expression denotes expression
T17391 29397-29402 Protein denotes GATA3
T17392 29460-29468 Negative_regulation denotes inhibits
T17393 29469-29474 Protein denotes FOXP3
T17394 29475-29485 Gene_expression denotes expression
T17395 29536-29540 Protein denotes IL-4
T17396 29549-29554 Protein denotes IL-13
T17397 29586-29593 Negative_regulation denotes inhibit
T17398 29586-29593 Negative_regulation denotes inhibit true
T17399 29594-29599 Protein denotes TGF-β
T17400 29609-29614 Protein denotes FOXP3
T17401 29615-29624 Positive_regulation denotes induction
T17402 29725-29729 Protein denotes IL-4
T17403 29800-29804 Protein denotes IL-4
T17404 29814-29818 Protein denotes IL-4
T17405 29902-29906 Protein denotes IL-4
T17406 29907-29911 Protein denotes IL-4
T17407 29991-29995 Protein denotes CD25
T17408 30001-30006 Protein denotes Foxp3
T17409 30061-30065 Protein denotes IL-4
T17410 30259-30266 Gene_expression denotes express
T17411 30267-30271 Protein denotes CD25
T17412 30313-30317 Protein denotes IL-4
T17413 30327-30334 Negative_regulation denotes inhibit true
T17414 30335-30340 Protein denotes Foxp3
T17415 30341-30351 Gene_expression denotes expression
T17416 30406-30416 Positive_regulation denotes contribute true
T17417 30433-30437 Negative_regulation denotes drop
T17418 30441-30446 Protein denotes Foxp3
T17419 30447-30457 Gene_expression denotes expression
T17420 30459-30463 Protein denotes IL-4
T17421 30559-30564 Protein denotes IL-17
T17422 30565-30574 Gene_expression denotes producing
T17423 30648-30652 Protein denotes IL-6
T17424 30675-30680 Protein denotes TGF-β
T17425 30891-30895 Protein denotes IL-4
T17426 30900-30904 Protein denotes IL-6
T17427 30906-30910 Protein denotes IL-4
T17428 30965-30970 Protein denotes FOXP3
T17429 30990-30993 Protein denotes CD4
T17430 30994-30998 Protein denotes CD25
T17431 31050-31054 Protein denotes IL-4
T17432 31095-31099 Protein denotes IL-4
T17433 31130-31138 Positive_regulation denotes enhanced
T17434 31139-31144 Protein denotes FOXP3
T17435 31145-31155 Gene_expression denotes expression
T17436 31214-31220 Positive_regulation denotes induce true
T17437 31221-31226 Protein denotes GATA3
T17438 31227-31237 Gene_expression denotes expression
T17439 31239-31243 Protein denotes IL-4
T17440 31300-31308 Regulation denotes regulate true
T17441 31309-31314 Protein denotes FOXP3
T17442 31315-31325 Gene_expression denotes expression
T17443 31366-31371 Protein denotes IL-13
T17444 31393-31399 Negative_regulation denotes reduce true
T17445 31400-31405 Protein denotes FOXP3
T17446 31419-31425 Positive_regulation denotes induce true
T17447 31426-31431 Protein denotes GATA3
T17448 31458-31462 Protein denotes IL-4
T17449 31473-31483 Negative_regulation denotes inhibition
T17450 31487-31492 Protein denotes FOXP3
T17451 31502-31510 Positive_regulation denotes mediated true
T17452 31514-31519 Protein denotes GATA3
T17453 31530-31535 Protein denotes GATA3
T17454 31536-31544 Positive_regulation denotes inducing
T17455 31545-31549 Protein denotes IL-4
T17456 31565-31574 Negative_regulation denotes repressed
T17457 31575-31580 Protein denotes TGF-β
T17458 31581-31589 Positive_regulation denotes mediated
T17459 31590-31595 Protein denotes FOXP3
T17460 31596-31606 Gene_expression denotes expression
T17461 31616-31620 Protein denotes IL-4
T17462 31632-31637 Protein denotes TGF-β
T17463 31715-31720 Protein denotes GATA3
T17464 31725-31730 Protein denotes FOXP3
T17465 31869-31873 Positive_regulation denotes high
T17466 31874-31879 Protein denotes GATA3
T17467 31880-31890 Gene_expression denotes expressing
T17468 31915-31922 Negative_regulation denotes absence
T17469 31926-31931 Protein denotes FOXP3
T17470 31957-31962 Protein denotes GATA3
T17471 31982-31991 Negative_regulation denotes inhibited
T17472 31992-31997 Protein denotes FOXP3
T17473 31998-32007 Positive_regulation denotes induction
T17474 32076-32081 Protein denotes GATA3
T17475 32131-32141 Gene_expression denotes expressing
T17476 32142-32147 Protein denotes GATA3
T17477 32168-32171 Protein denotes CD2
T17478 32172-32177 Protein denotes GATA3
T17479 32223-32237 Positive_regulation denotes overexpression
T17480 32241-32246 Protein denotes GATA3
T17481 32285-32291 Negative_regulation denotes failed
T17482 32295-32301 Positive_regulation denotes induce
T17483 32302-32307 Protein denotes FOXP3
T17484 32308-32318 Gene_expression denotes expression
T17485 32365-32370 Protein denotes TGF-β
T17486 32396-32399 Protein denotes CD2
T17487 32400-32405 Protein denotes GATA3
T17488 32430-32435 Protein denotes FOXP3
T17489 32535-32540 Protein denotes GATA3
T17490 32775-32780 Protein denotes GATA3
T17491 32781-32795 Positive_regulation denotes overexpression
T17492 32874-32879 Protein denotes GATA3
T17493 32903-32908 Protein denotes GATA3
T17494 32982-33000 Protein denotes Repressor of GATA3
T17495 33002-33005 Protein denotes ROG
T17496 33045-33048 Protein denotes CD8
T17497 33196-33201 Protein denotes GATA3
T17498 33287-33292 Protein denotes GATA3
T17499 33293-33302 Negative_regulation denotes repressed
T17500 33303-33308 Protein denotes FOXP3
T17501 33309-33319 Gene_expression denotes expression
T17502 33332-33339 Binding denotes binding
T17503 33347-33352 Protein denotes FOXP3
T17504 33353-33368 Entity denotes promoter region
T17505 33505-33510 Protein denotes FOXP3
T17506 33706-33713 Gene_expression denotes express
T17507 33714-33719 Protein denotes GATA3
T17508 33783-33790 Gene_expression denotes express true
T17509 33791-33796 Protein denotes GATA3
T17510 33831-33836 Binding denotes bound
T17511 33840-33845 Protein denotes GATA3
T17512 33930-33935 Protein denotes GATA3
T17513 33936-33941 Binding denotes binds
T17514 33994-33999 Protein denotes GATA3
T17515 34203-34208 Protein denotes GATA3
T17516 34209-34219 Gene_expression denotes expression
T17517 34220-34223 Negative_regulation denotes low
T17518 34262-34267 Protein denotes FOXP3
T17519 34328-34333 Protein denotes GATA3
T17520 34337-34344 Negative_regulation denotes inhibit
T17521 34345-34350 Protein denotes FOXP3
T17522 34490-34495 Protein denotes GATA3
T17523 34500-34507 Negative_regulation denotes compete true
T17524 34500-34507 Negative_regulation denotes compete true
T17525 34517-34524 Binding denotes binding
T17526 34517-34524 Binding denotes binding
T17527 34528-34532 Protein denotes AP-1
T17528 34533-34537 Protein denotes NFAT
T17529 34545-34553 Entity denotes promoter
T17530 34615-34620 Protein denotes FOXP3
T17531 34624-34644 Negative_regulation denotes negatively regulated
T17532 34666-34670 Protein denotes IL-4
T17533 34672-34677 Protein denotes GATA3
T17534 34678-34698 Negative_regulation denotes acts as an inhibitor
T17535 34702-34707 Protein denotes FOXP3
T17536 34708-34718 Gene_expression denotes expression
T17537 34803-34810 Binding denotes binding
T17538 34815-34825 Negative_regulation denotes repressing
T17539 34830-34835 Protein denotes FOXP3
T17540 34836-34844 Entity denotes promoter
T17541 34891-34895 Protein denotes Il-4
T17542 34926-34936 Negative_regulation denotes repressing
T17543 34937-34942 Protein denotes FOXP3
T17544 34943-34953 Gene_expression denotes expression
T18037 35386-35396 Gene_expression denotes expressing
T18038 35397-35403 Protein denotes GATA-3
T18039 35425-35428 Protein denotes CD2
T18040 35451-35454 Protein denotes CD2
T18041 35455-35460 Protein denotes GATA3
T18042 35489-35492 Protein denotes CD2
T18043 35493-35498 Protein denotes GATA3
T18746 36389-36394 Protein denotes FOXP3
T18747 36448-36453 Protein denotes FOXP3
T18748 36499-36504 Protein denotes EF-1α
T18749 36558-36563 Protein denotes GATA3
T18750 36977-36988 Protein denotes Ubiquitin C
T18751 37062-37068 Protein denotes Smad-7
T18752 37141-37147 Protein denotes TGF-ß1
T18753 37568-37579 Protein denotes ubiquitin C
T19471 38323-38326 Protein denotes CD4
T19472 38328-38334 Protein denotes CD45RA
T19473 38357-38363 Protein denotes CD45RO
T19474 38364-38373 Negative_regulation denotes depletion
T19475 38484-38487 Protein denotes CD3
T19476 38519-38523 Protein denotes CD28
T19477 38562-38567 Protein denotes IL-12
T19478 38582-38586 Protein denotes IL-4
T19479 38630-38634 Protein denotes IL-4
T19480 38649-38654 Protein denotes IFN-γ
T19481 38669-38674 Protein denotes IL-12
T19482 38722-38727 Protein denotes TGF-β
T19483 38742-38747 Protein denotes IFN-γ
T19484 38762-38767 Protein denotes IL-12
T19485 38782-38786 Protein denotes IL-4
T19486 38843-38847 Protein denotes IL-2
T20691 40998-41002 Protein denotes CD28
T20692 41007-41012 Protein denotes TGF-β
T20693 41039-41042 Protein denotes TAT
T20694 41086-41091 Protein denotes FOXP3
T20695 41092-41102 Gene_expression denotes expression true
T20887 41316-41320 Protein denotes IL-4
T21708 41610-41615 Protein denotes FOXP3
T21709 41616-41626 Gene_expression denotes expression true
T21710 41707-41711 Protein denotes CD25
T21711 41851-41856 Protein denotes FOXP3
T21712 42081-42086 Protein denotes CD152
T21713 42109-42113 Protein denotes PD-1
T21714 42135-42139 Protein denotes GITR
T21715 42162-42166 Protein denotes CD69
T21716 42191-42196 Protein denotes CD103
T21717 42220-42225 Protein denotes CD62L
T21718 42483-42486 Protein denotes CD3
T21719 42493-42496 Protein denotes CD4
T21720 42507-42511 Protein denotes CD25
T21721 42646-42649 Protein denotes CD4
T21722 42689-42692 Protein denotes CD2
T21723 42693-42698 Protein denotes GATA3
T21724 42737-42741 Protein denotes CD25
T21725 42753-42758 Protein denotes CD62L
T21726 42772-42775 Protein denotes CD4
T21727 42885-42890 Protein denotes Foxp3
T21728 42891-42901 Gene_expression denotes expression true
T21729 42975-42980 Protein denotes Foxp3
T21730 43050-43053 Protein denotes CD4
T22201 43267-43272 Protein denotes FOXP3
T22202 43312-43315 Protein denotes CD4
T22203 43316-43320 Protein denotes CD25
T22204 43651-43656 Protein denotes FOXP3
T22205 43941-43946 Protein denotes GATA3
T22206 43988-43993 Protein denotes T-bet
T22207 44033-44038 Protein denotes GAPDH
T22208 44067-44072 Protein denotes SMAD2
T22209 44095-44100 Protein denotes STAT6
T22210 44117-44122 Protein denotes STAT6
T22537 44335-44339 Protein denotes IL-4
T22538 44388-44392 Protein denotes IL-4
T22539 44409-44413 Protein denotes IL-4
T22540 44521-44524 Protein denotes CD3
T22541 44526-44529 Protein denotes CD4
T22542 44535-44539 Protein denotes CD25
T22543 44540-44550 Gene_expression denotes expression true
T22544 44540-44550 Gene_expression denotes expression true
T22545 44540-44550 Gene_expression denotes expression true
T22546 44567-44571 Protein denotes IL-4
T22547 44636-44640 Protein denotes IL-4
T22957 45111-45116 Protein denotes GATA3
T22958 45490-45495 Protein denotes FOXP3
T23424 45716-45719 Protein denotes CD4
T23425 46599-46604 Protein denotes GATA3
T23765 7404-7410 Positive_regulation denotes Induce true
T23766 7411-7416 Protein denotes FOXP3
T23767 7417-7427 Gene_expression denotes Expression
T23768 7483-7486 Protein denotes CD3
T23769 7487-7491 Protein denotes CD28
T23770 7508-7513 Protein denotes TGF-β
T23771 7587-7592 Protein denotes FOXP3
T23772 7763-7766 Protein denotes CD3
T23773 7767-7771 Protein denotes CD28
T23774 7773-7778 Protein denotes TGF-β
T23775 7788-7792 Protein denotes IL-4
T24033 10157-10164 Gene_expression denotes Express true
T24034 10165-10170 Protein denotes FOXP3
T24035 10206-10211 Protein denotes FOXP3
T24036 10212-10222 Gene_expression denotes expression true
T24037 10342-10347 Protein denotes TGF-β
T24038 10349-10354 Protein denotes FOXP3
T24039 10355-10365 Gene_expression denotes expression true
T24593 10632-10636 Protein denotes IL-4
T24594 10637-10646 Gene_expression denotes Producing
T24595 10653-10657 Negative_regulation denotes Lack
T24596 10658-10663 Protein denotes FOXP3
T24597 10699-10704 Protein denotes FOXP3
T24598 10709-10713 Protein denotes IL-4
T24599 10714-10724 Gene_expression denotes expression true
T24600 10714-10724 Gene_expression denotes expression true
T24601 10762-10765 Protein denotes CD4
T24602 10802-10808 Protein denotes CD45RO
T24603 10813-10817 Protein denotes CD25
T24604 10826-10836 Gene_expression denotes expression
T24605 10826-10836 Gene_expression denotes expression
T24606 10892-10898 Protein denotes CD45RO
T24607 10899-10903 Protein denotes CD25
T24608 10926-10932 Protein denotes CD45RO
T24609 10933-10937 Protein denotes CD25
T24610 10962-10968 Protein denotes CD45RO
T24611 10969-10973 Protein denotes CD25
T24612 11243-11248 Protein denotes CRTH2
T24613 11261-11265 Protein denotes IL-4
T24614 11266-11275 Gene_expression denotes secreting
T24615 11303-11309 Protein denotes CD45RO
T24616 11316-11323 Gene_expression denotes stained
T24617 11316-11323 Gene_expression denotes stained
T24618 11328-11333 Protein denotes FOXP3
T24619 11338-11342 Protein denotes IL-4
T25024 13213-13218 Protein denotes FOXP3
T25025 13219-13228 Positive_regulation denotes Induction
T25026 13274-13277 Protein denotes CD4
T25027 13278-13284 Protein denotes CD45RA
T25028 13331-13334 Protein denotes CD3
T25029 13335-13339 Protein denotes CD28
T25030 13359-13364 Protein denotes TGF-β
T25031 13378-13382 Protein denotes IL-4
T25032 13491-13496 Protein denotes FOXP3
T25033 13501-13506 Protein denotes GATA3
T25034 13507-13517 Gene_expression denotes expression true
T25035 13507-13517 Gene_expression denotes expression true
T25036 13596-13601 Protein denotes GATA3
T25037 13606-13611 Protein denotes FOXP3
T25038 13612-13620 Gene_expression denotes staining true
T25039 13612-13620 Gene_expression denotes staining true
T25040 13648-13651 Protein denotes CD4
T25041 13652-13658 Protein denotes CD45RA
T25761 16498-16504 Regulation denotes Effect
T25762 16508-16512 Protein denotes IL-4
T25763 16516-16521 Protein denotes FOXP3
T25764 16522-16531 Positive_regulation denotes Induction
T25765 16599-16604 Protein denotes TGF-β
T25766 16636-16640 Protein denotes IL-4
T25767 16869-16872 Protein denotes CD4
T25768 16873-16879 Protein denotes CD45RA
T25769 16949-16954 Protein denotes TGF-β
T25770 16973-16983 Positive_regulation denotes increasing
T25771 17001-17005 Protein denotes IL-4
T25772 17081-17084 Protein denotes CD4
T25773 17085-17091 Protein denotes CD45RA
T25774 17146-17149 Protein denotes CD3
T25775 17150-17154 Protein denotes CD28
T25776 17156-17161 Protein denotes TGF-β
T25777 17177-17181 Protein denotes IL-4
T25778 17279-17293 Phosphorylation denotes phosphorylated true
T25779 17279-17293 Phosphorylation denotes phosphorylated true
T25780 17294-17299 Protein denotes SMAD2
T25781 17304-17309 Protein denotes STAT6
T25782 17317-17322 Protein denotes STAT6
T25783 17327-17332 Protein denotes GAPDH
T25784 17379-17384 Protein denotes GATA3
T25785 17389-17394 Protein denotes FOXP3
T25786 17395-17403 Gene_expression denotes staining
T25787 17395-17403 Gene_expression denotes staining
T25788 17432-17435 Protein denotes CD4
T25789 17436-17442 Protein denotes CD45RA
T25790 17455-17459 Protein denotes IL-4
T26231 18665-18669 Protein denotes IL-4
T26232 18679-18684 Protein denotes TGF-β
T26233 18724-18727 Protein denotes CD4
T26234 18728-18734 Protein denotes CD45RA
T26235 18779-18782 Protein denotes CD3
T26236 18783-18787 Protein denotes CD28
T26237 18789-18794 Protein denotes TGF-β
T26238 18800-18804 Protein denotes IL-4
T26239 19101-19106 Protein denotes GATA3
T26240 19111-19116 Protein denotes FOXP3
T26241 19117-19125 Gene_expression denotes staining true
T26242 19117-19125 Gene_expression denotes staining true
T26243 19168-19171 Protein denotes CD4
T26244 19172-19178 Protein denotes CD45RA
T26245 19196-19199 Protein denotes CD3
T26246 19200-19202 Protein denotes 28
T26247 19204-19208 Protein denotes IL-4
T26248 19213-19218 Protein denotes TGF-β
T26810 22450-22455 Protein denotes GATA3
T26811 22488-22493 Protein denotes FOXP3
T26812 22521-22524 Protein denotes CD4
T26813 22525-22529 Protein denotes CDRA
T26814 22586-22595 Protein denotes TAT-GATA3
T26815 22623-22631 Gene_expression denotes presence true
T26816 22635-22640 Protein denotes GATA3
T26817 22680-22683 Protein denotes CD3
T26818 22684-22688 Protein denotes CD28
T26819 22714-22717 Protein denotes GFP
T26820 22775-22780 Protein denotes FOXP3
T26821 22781-22791 Gene_expression denotes expression true
T26822 22902-22905 Protein denotes CD4
T26823 22906-22910 Protein denotes CD25
T26824 22959-22962 Protein denotes CD2
T26825 22963-22968 Protein denotes GATA3
T26826 22999-23004 Protein denotes TGF-β
T26827 23023-23026 Protein denotes CD4
T26828 23045-23050 Protein denotes FOXP3
T26829 23230-23235 Protein denotes SMAD7
T26830 23240-23245 Protein denotes TGF-β
T26831 23246-23256 Gene_expression denotes expression true
T26832 23246-23256 Gene_expression denotes expression true
T27678 25937-25942 Protein denotes GATA3
T27679 25943-25952 Negative_regulation denotes Represses
T27680 25963-25968 Protein denotes FOXP3
T27681 25969-25977 Entity denotes Promoter
T27682 26019-26022 Protein denotes CD4
T27683 26116-26121 Protein denotes FOXP3
T27684 26151-26161 Protein denotes luciferase
T27685 26249-26259 Protein denotes luciferase
T27686 26341-26344 Protein denotes CD4
T27687 26379-26384 Protein denotes FOXP3
T27688 26429-26434 Protein denotes GATA3
T27689 26435-26445 Gene_expression denotes expression
T27690 26578-26581 Protein denotes CD4
T27691 26627-26632 Protein denotes GATA3
T27692 26645-26650 Protein denotes FOXP3
T27693 26789-26799 Protein denotes luciferase
T27694 26939-26944 Protein denotes GATA3
T27695 27154-27161 Gene_expression denotes amounts true
T27696 27165-27170 Protein denotes GATA3
T27697 27241-27246 Protein denotes GATA3
T27698 27407-27412 Protein denotes GATA3
T27699 27413-27420 Binding denotes binding true
T27700 27428-27433 Protein denotes FOXP3
T27701 27434-27442 Entity denotes promoter
T752 0-5 Protein denotes GATA3
T753 27-34 Negative_regulation denotes Inhibit
T754 35-41 Protein denotes TGF-β1
T755 42-49 Positive_regulation denotes Induced
T756 50-55 Protein denotes FOXP3
T757 56-66 Gene_expression denotes Expression
T758 388-393 Protein denotes FOXP3
T759 530-540 Regulation denotes regulating true
T760 545-555 Gene_expression denotes expression
T761 708-721 Protein denotes interleukin 4
T762 723-727 Protein denotes IL-4
T763 767-775 Negative_regulation denotes inhibits
T764 776-781 Protein denotes FOXP3
T765 875-889 Gene_expression denotes overexpressing
T766 890-896 Protein denotes GATA-3
T767 931-940 Negative_regulation denotes deficient
T18290 35795-35798 Protein denotes CD4
T18291 35809-35812 Protein denotes CD4
T18292 35890-35893 Protein denotes CD4
T18293 35979-35982 Protein denotes CD4
T18294 36177-36181 Positive_regulation denotes high
T18295 36182-36186 Protein denotes IL-4
T18296 36187-36196 Gene_expression denotes secretion
T19044 37820-37823 Protein denotes CD4
T19045 37834-37837 Protein denotes CD4
T19046 37840-37845 Protein denotes CD62L
T19047 37852-37856 Protein denotes CD25
T19048 38120-38126 Protein denotes TGF-ß1
T19049 38221-38226 Protein denotes FOXP3
T19050 38227-38237 Gene_expression denotes expression true
T19733 38876-38881 Protein denotes FOXP3
T19734 38934-38939 Protein denotes FOXP3
T19735 39026-39031 Protein denotes FOXP3
T19736 39081-39086 Protein denotes FOXP3
T20030 39467-39472 Protein denotes FOXP3
T20031 39482-39492 Protein denotes luciferase
T20032 39550-39553 Protein denotes CD4
T20033 39779-39789 Protein denotes luciferase
T20034 39824-39834 Protein denotes luciferase
T20035 39960-39970 Protein denotes luciferase
T20392 40294-40297 Protein denotes TAT
T20393 40327-40332 Protein denotes GATA3
T20394 40358-40363 Protein denotes GATA3
T20395 40365-40372 Negative_regulation denotes lacking
T20396 40377-40393 Entity denotes two zinc fingers
T20397 40457-40460 Protein denotes TAT
T20398 40631-40634 Protein denotes TAT
T20685 40747-40750 Protein denotes TAT
T20686 40751-40756 Protein denotes GATA3
T20687 40771-40774 Protein denotes CD4
T20688 40775-40781 Protein denotes CD45RA
T20689 40890-40895 Protein denotes GATA3
T20690 40985-40988 Protein denotes CD3
R607 T780 T778 themeOf IL-4,target
R608 T781 T779 themeOf GATA3,target
R653 T754 T755 causeOf TGF-β1,Induced
R654 T755 T753 themeOf Induced,Inhibit
R655 T756 T757 themeOf FOXP3,Expression
R656 T757 T755 themeOf Expression,Induced
R658 T760 T759 themeOf expression,regulating
R660 T762 T761 equivalentTo IL-4,interleukin 4
R2114 T2517 T2519 causeOf interleukin 4,induced
R2115 T2517 T2520 causeOf interleukin 4,induced
R2116 T2518 T2517 equivalentTo IL-4,interleukin 4
R2118 T2521 T2519 themeOf STAT6,induced
R2119 T2522 T2520 themeOf GATA3,induced
R2121 T2523 T2524 causeOf GATA3,induction
R2122 T2523 T2526 causeOf GATA3,induction
R2123 T2523 T2525 causeOf GATA3,induction
R2125 T2527 T2525 themeOf IL-4,induction
R2126 T2528 T2524 themeOf IL-5,induction
R2127 T2529 T2526 themeOf IL-13,induction
R2129 T2530 T2531 causeOf T-bet,modulates
R2130 T2532 T2531 themeOf GATA3,modulates
R2131 T2536 T2535 themeOf GATA3,Overexpression of a dominant negative form
R2132 T2537 T2538 themeOf GATA3,blockade
R2134 T2540 T2539 themeOf FOXP3,express
R2136 T2542 T2541 themeOf CD25,express
R2137 T2544 T2543 themeOf FOXP3,express
R2141 T2546 T2547 themeOf FOXP3,expression
R2143 T2547 T2545 themeOf expression,induce
R2145 T2550 T2549 themeOf TGF-βRII,lacking
R2147 T2556 T2557 causeOf GATA3,inhibits
R2148 T2558 T2559 themeOf FOXP3,induction
R2149 T2559 T2557 themeOf induction,inhibits
R2151 T2560 T2561 causeOf IL-4,limits
R2152 T2561 T2565 themeOf limits,mediated way
R2153 T2562 T2563 themeOf FOXP3,expression
R2155 T2563 T2561 themeOf expression,limits
R2156 T2564 T2565 causeOf GATA3,mediated way
R2158 T2566 T2567 themeOf GATA3,binds
R2159 T2567 T2570 causeOf binds,prevents
R2161 T2568 T2571 themeOf FOXP3,induction
R2162 T2569 T2567 themeOf promoter,binds
R2163 T2569 T2568 partOf promoter,FOXP3
R2165 T2571 T2570 themeOf induction,prevents
R3817 T4838 T4839 themeOf FOXP3,Induction
R3822 T4840 T4841 themeOf FOXP3,expression
R3824 T4841 T4842 themeOf expression,induced
R3825 T4845 T4844 themeOf FOXP3,express
R3826 T4846 T4847 themeOf FOXP3,expressed
R3827 T4846 T4848 themeOf FOXP3,expression
R3828 T4848 T4849 themeOf expression,restricted
R3829 T4889 T4890 themeOf FOXP3,expression
R3830 T4850 T4851 themeOf FOXP3,mRNA expression
R3831 T4890 T4888 themeOf expression,lack
R3832 T4892 T4891 themeOf FOXP3,expressed
R3833 T4864 T4863 themeOf FOXP3,induce
R3834 T4894 T4893 themeOf FOXP3,express
R3835 T4896 T4897 themeOf CD25,expression
R3836 T4897 T4895 themeOf expression,low
R3837 T4898 T4899 causeOf TGF-β,increase
R3838 T4900 T4901 themeOf FOXP3,expression
R3839 T4901 T4899 themeOf expression,increase
R3840 T4903 T4904 themeOf FOXP3,expression
R3841 T4904 T4902 themeOf expression,induced
R3842 T4906 T4907 themeOf IL-4,secretion
R3843 T4908 T4909 themeOf IL-4,producing
R3844 T4865 T4863 causeOf TGF-β,induce
R3845 T4911 T4912 themeOf FOXP3,expression
R3846 T4912 T4910 themeOf expression,lacking
R3847 T4913 T4915 causeOf TGF-β,induction
R3848 T4867 T4868 themeOf FOXP3,expression
R3849 T4914 T4915 themeOf FOXP3,induction
R3850 T4915 T4916 themeOf induction,failed
R3851 T4868 T4866 themeOf expression,increase
R3852 T4920 T4918 themeOf IL-4,expression
R3853 T4870 T4869 themeOf FOXP3,up-regulate
R3854 T4921 T4919 themeOf FOXP3,expression
R3855 T4923 T4924 themeOf IL-4,expressing
R3856 T4872 T4871 themeOf IL-4,produce
R3857 T4872 T4874 causeOf IL-4,prevent
R3858 T4928 T4927 themeOf FOXP3,co-express
R3859 T4875 T4876 themeOf FOXP3,induction
R3860 T4932 T4931 themeOf FOXP3,expressed
R3861 T4934 T4933 themeOf IL-4,lacking
R3862 T4876 T4874 themeOf induction,prevent
R3863 T4877 T4880 causeOf neutralization,rescue
R3864 T4878 T4877 themeOf IL-4,neutralization
R3865 T4881 T4882 themeOf FOXP3,expression
R3866 T4947 T4946 themeOf FOXP3,expressed
R3867 T4882 T4880 themeOf expression,rescue
R3868 T4884 T4883 themeOf FOXP3,express
R3869 T4948 T4949 themeOf IL-4,secretion
R3870 T4886 T4885 themeOf express,inability
R3871 T4950 T4951 themeOf FOXP3,expressing
R3872 T4952 T4953 themeOf FOXP3,expressed
R3873 T4952 T4954 themeOf FOXP3,inducible
R3874 T4887 T4886 themeOf FOXP3,express
R4733 T6109 T6110 themeOf FOXP3,expression
R4734 T6110 T6108 themeOf expression,induce
R4735 T6113 T6112 themeOf FOXP3,expression
R4736 T6114 T6111 themeOf GATA3,expression
R4737 T6119 T6122 themeOf FOXP3,expression
R4738 T6120 T6121 themeOf GATA3,expression
R4739 T6123 T6124 themeOf FOXP3,mRNA expression
R4740 T6124 T6125 themeOf mRNA expression,increased
R4741 T6126 T6129 themeOf GATA3,expression
R4742 T6127 T6128 themeOf FOXP3,expression
R4743 T6130 T6131 themeOf expressing,more
R4744 T6132 T6130 themeOf FOXP3,expressing
R4745 T6133 T6134 themeOf expressed,low
R4746 T6135 T6133 themeOf GATA3,expressed
R4747 T6137 T6141 themeOf CD103,expression
R4748 T6138 T6144 themeOf CTLA-4,expression
R4749 T6139 T6143 themeOf GITR,expression
R4750 T6140 T6142 themeOf PD-1,expression
R4751 T6145 T6148 themeOf GATA3,expression
R4752 T6146 T6147 themeOf FOXP3,expression
R4753 T6147 T6149 themeOf expression,resolved
R4754 T6148 T6150 themeOf expression,resolved
R4755 T6152 T6151 themeOf express,lost
R4756 T6153 T6152 themeOf FOXP3,express
R6096 T7948 T7949 causeOf IL-4,inhibit
R6098 T7950 T7951 causeOf TGF-β,induction
R6099 T7951 T7949 themeOf induction,inhibit
R6100 T7952 T7951 themeOf FOXP3,induction
R6104 T7959 T7960 causeOf IL-4,repressed
R6105 T7961 T7962 causeOf TGF-β,induction
R6106 T7962 T7960 themeOf induction,repressed
R6108 T7963 T7964 themeOf FOXP3,expression
R6109 T7964 T7962 themeOf expression,induction
R6110 T7965 T7966 themeOf GATA3,induced
R6112 T7967 T7966 causeOf absence,induced
R6113 T7968 T7967 themeOf IL-4,absence
R6114 T7969 T7970 causeOf IL-4,induce
R6116 T8032 T8033 themeOf FOXP3,expression
R6117 T7971 T7972 themeOf FOXP3,expression
R6118 T7972 T7970 themeOf expression,induce
R6119 T7973 T7974 themeOf GATA3,induced
R6120 T7973 T7975 themeOf GATA3,induced
R6121 T7976 T7974 causeOf TGF-β,induced
R6122 T7977 T7975 causeOf IL-4,induced
R6123 T7978 T7979 causeOf IL-4,prevention
R6124 T7979 T7982 themeOf prevention,caused
R6125 T7980 T7981 themeOf FOXP3,expression
R6126 T7981 T7979 themeOf expression,prevention
R6127 T7983 T7987 themeOf phosphorylation,affected
R6128 T7983 T7988 themeOf phosphorylation,affected
R6129 T7984 T7989 themeOf phosphorylation,affected
R6130 T7984 T7990 themeOf phosphorylation,affected
R6131 T7985 T7983 themeOf SMAD2,phosphorylation
R6132 T7986 T7984 themeOf STAT6,phosphorylation
R6133 T8033 T8031 themeOf expression,decrease
R6134 T7991 T7987 causeOf IL-4,affected
R6135 T7991 T7989 causeOf IL-4,affected
R6136 T7992 T7988 causeOf TGF-β,affected
R6137 T7992 T7990 causeOf TGF-β,affected
R6138 T8034 T8031 causeOf IL-4,decrease
R6139 T7995 T7997 causeOf Increasing amounts,increase
R6140 T7995 T8000 causeOf Increasing amounts,decreased
R6141 T7996 T7995 themeOf IL-4,Increasing amounts
R6143 T7998 T7997 themeOf GATA3,increase
R6144 T7999 T8000 themeOf FOXP3,decreased
R6145 T8003 T8002 themeOf CD25,express
R6146 T8005 T8004 equivalentTo rmIL-4,IL-4
R6151 T8022 T8025 causeOf IL-4,suppressive capacity
R6152 T8023 T8024 themeOf FOXP3,expression
R6153 T8024 T8025 themeOf expression,suppressive capacity
R6154 T8026 T8027 causeOf IL-4,inhibit
R6156 T8027 T8030 themeOf inhibit,altered
R6157 T8028 T8029 themeOf FOXP3,expression
R6158 T8029 T8027 themeOf expression,inhibit
R7207 T9436 T9439 causeOf TGF-β,reduced
R7208 T9436 T9437 causeOf TGF-β,reduced
R7209 T9436 T9438 causeOf TGF-β,reduced
R7210 T9440 T9442 themeOf CD25,expression
R7211 T9441 T9443 themeOf IL-4,expression
R7212 T9442 T9437 themeOf expression,reduced
R7213 T9443 T9438 themeOf expression,reduced
R7214 T9444 T9445 themeOf CD25,expression
R7215 T9445 T9439 themeOf expression,reduced
R7216 T9446 T9447 causeOf IL-4,inhibited
R7217 T9448 T9449 causeOf TGF-β,induction
R7218 T9449 T9447 themeOf induction,inhibited
R7219 T9450 T9449 themeOf FOXP3,induction
R7220 T9458 T9459 causeOf TGF-β,induction
R7221 T9459 T9462 themeOf induction,caused
R7222 T9460 T9461 themeOf FOXP3,expression
R7223 T9461 T9459 themeOf expression,induction
R7224 T9469 T9470 themeOf CD25,down-regulated
R7225 T9470 T9472 themeOf down-regulated,pronounced
R7226 T9471 T9470 causeOf TGF-β,down-regulated
R7227 T9474 T9472 causeOf IL-4,pronounced
R7228 T9477 T9478 themeOf IL-4,producing
R7229 T9479 T9480 themeOf FOXP3,expressing
R7230 T9482 T9484 themeOf GATA3,expression
R7231 T9483 T9485 themeOf FOXP3,expression
R7232 T9486 T9488 causeOf IL-4,demonstrated
R7233 T9486 T9490 causeOf IL-4,demonstrated
R7234 T9487 T9489 causeOf TGF-β,demonstrated
R7235 T9487 T9491 causeOf TGF-β,demonstrated
R7236 T9492 T9494 themeOf GATA3,co-express
R7237 T9493 T9495 themeOf FOXP3,co-express
R7238 T9494 T9488 themeOf co-express,demonstrated
R7239 T9494 T9489 themeOf co-express,demonstrated
R7240 T9495 T9490 themeOf co-express,demonstrated
R7241 T9495 T9491 themeOf co-express,demonstrated
R7242 T9496 T9497 causeOf GATA3,repressing
R7243 T9498 T9497 themeOf FOXP3,repressing
R7244 T9499 T9500 causeOf IL-4,acts in vitro as an inhibitor
R7245 T9499 T9506 causeOf IL-4,dependent
R7246 T9500 T9504 themeOf acts in vitro as an inhibitor,acting
R7247 T9501 T9502 themeOf FOXP3,expression
R7248 T9502 T9500 themeOf expression,acts in vitro as an inhibitor
R7249 T9505 T9506 themeOf GATA3,dependent
R7250 T9506 T9504 causeOf dependent,acting
R8417 T11187 T11188 causeOf GATA3,Negative Regulator
R8418 T11189 T11190 themeOf FOXP3,Expression
R8419 T11190 T11188 themeOf Expression,Negative Regulator
R8420 T11191 T11192 themeOf FOXP3,expression
R8421 T11192 T11193 themeOf expression,decreased
R8422 T11194 T11195 themeOf GATA3,expression
R8423 T11195 T11193 causeOf expression,decreased
R8424 T11195 T11196 themeOf expression,high
R8425 T11198 T11197 causeOf GATA3,role
R8426 T11199 T11197 themeOf repressing,role
R8427 T11200 T11199 themeOf FOXP3,repressing
R8428 T11202 T11203 causeOf GATA3,inhibiting
R8429 T11204 T11205 themeOf STAT4,expression
R8430 T11205 T11203 themeOf expression,inhibiting
R8431 T11207 T11208 causeOf GATA3,inhibit
R8432 T11209 T11210 themeOf FOXP3,induction
R8433 T11210 T11208 themeOf induction,inhibit
R8434 T11214 T11213 themeOf DNA-binding domain,lacking
R8435 T11214 T11212 partOf DNA-binding domain,GATA3
R8436 T11223 T11224 causeOf TAT-GATA3,reduced
R8437 T11225 T11226 themeOf FOXP3,expression
R8438 T11226 T11224 themeOf expression,reduced
R8439 T11227 T11228 themeOf DNA-binding domain,truncated
R8440 T11227 T11229 partOf DNA-binding domain,GATA3
R8441 T11228 T11230 causeOf truncated,affect
R8442 T11231 T11232 themeOf FOXP3,expression
R8443 T11232 T11230 themeOf expression,affect
R8444 T11234 T11233 causeOf GATA3,inhibitory effect
R8445 T11235 T11233 themeOf FOXP3,inhibitory effect
R8446 T11237 T11236 themeOf GATA3,overexpressing
R8447 T11245 T11244 causeOf GATA3,effect
R8448 T11250 T11251 themeOf Foxp3,expression
R8449 T11251 T11244 themeOf expression,effect
R8450 T11255 T11256 causeOf TGF-β,up-regulated
R8451 T11257 T11256 themeOf Foxp3,up-regulated
R8452 T11259 T11260 causeOf GATA3,reduced
R8453 T11261 T11262 themeOf Foxp3,expression
R8454 T11262 T11260 themeOf expression,reduced
R8455 T11262 T11263 themeOf expression,activated
R8456 T11264 T11263 causeOf TGF-β,activated
R8457 T11266 T11265 themeOf TGF-β,produced
R8458 T11267 T11268 themeOf Smad7,expressed
R8459 T11270 T11271 causeOf IL-4,induced
R8460 T11272 T11271 themeOf GATA3,induced
R9656 T12887 T12888 causeOf GATA3,Represses
R9657 T12890 T12888 themeOf Promoter,Represses
R9658 T12890 T12889 partOf Promoter,FOXP3
R9659 T12891 T12892 causeOf GATA3,repression
R9660 T12893 T12892 themeOf FOXP3,repression
R9661 T12896 T12897 causeOf GATA-binding site,play an important role
R9662 T12896 T12894 partOf GATA-binding site,FOXP3
R9663 T12898 T12899 themeOf FOXP3,regulation
R9664 T12899 T12897 themeOf regulation,play an important role
R9665 T12903 T12902 themeOf GATA3,express
R9666 T12905 T12908 themeOf promoter,active
R9667 T12905 T12904 partOf promoter,FOXP3
R9668 T12906 T12907 themeOf GATA3,expressing
R9669 T12910 T12908 causeOf express,active
R9670 T12911 T12910 themeOf GATA3,express
R9671 T12913 T12912 themeOf GATA3,Overexpression
R9672 T12916 T12915 themeOf GATA3-binding site,deleting
R9673 T12916 T12914 partOf GATA3-binding site,FOXP3
R9674 T12923 T12922 causeOf GATA3,repressor
R9675 T12925 T12922 themeOf promoter,repressor
R9676 T12925 T12924 partOf promoter,FOXP3
R9677 T12926 T12927 themeOf GATA3,binds
R9678 T12929 T12927 themeOf promoter,binds
R9679 T12929 T12928 partOf promoter,FOXP3
R9680 T12932 T12933 themeOf GATA3,binding
R9681 T12934 T12935 themeOf GATA3,expressing
R9682 T12937 T12936 themeOf GATA3,overexpressing
R9683 T12938 T12939 themeOf GATA3,binding
R9684 T12940 T12941 themeOf GATA3,binds
R9685 T12943 T12941 themeOf promoter,binds
R9686 T12943 T12942 partOf promoter,FOXP3
R9687 T12945 T12946 themeOf GATA3,binds
R9688 T12948 T12946 themeOf promoter region,binds
R9689 T12948 T12947 partOf promoter region,FOXP3
R9690 T12949 T12950 themeOf GATA3,binding
R9691 T12950 T12952 causeOf binding,repressing
R9692 T12951 T12950 themeOf FOXP3,binding
R9693 T12953 T12954 themeOf FOXP3,expression
R9694 T12954 T12952 themeOf expression,repressing
R13155 T17339 T17340 themeOf FOXP3,induction
R13156 T17340 T17341 themeOf induction,inhibited
R13157 T17342 T17341 causeOf GATA3,inhibited
R13160 T17347 T17346 themeOf FOXP3,up-regulate
R13161 T17348 T17349 themeOf FOXP3,induction
R13163 T17351 T17350 themeOf FOXP3,up-regulate
R13164 T17352 T17350 causeOf TGF-β,up-regulate
R13166 T17354 T17356 themeOf T-bet,expression
R13167 T17354 T17359 themeOf T-bet,co-expression
R13168 T17354 T17361 themeOf T-bet,expression
R13170 T17355 T17357 themeOf GATA3,expression
R13171 T17355 T17360 themeOf GATA3,co-expression
R13172 T17355 T17362 themeOf GATA3,expression
R13175 T17363 T17369 causeOf TGF-β,low
R13176 T17363 T17370 causeOf TGF-β,low
R13177 T17365 T17369 themeOf expression,low
R13178 T17366 T17370 themeOf expression,low
R13179 T17367 T17365 themeOf GATA3,expression
R13181 T17368 T17366 themeOf T-bet,expression
R13184 T17374 T17371 themeOf IL-4,absence
R13185 T17375 T17372 themeOf IL-12,absence
R13186 T17376 T17373 themeOf TGF-β,absence
R13187 T17377 T17378 themeOf FOXP3,expression
R13188 T17379 T17380 themeOf FOXP3,expressing
R13190 T17381 T17383 themeOf GATA3,up-regulated
R13191 T17382 T17384 themeOf T-bet,up-regulated
R13193 T17385 T17386 themeOf FOXP3,promoting
R13195 T17387 T17386 causeOf CTLA-4,promoting
R13196 T17387 T17388 causeOf CTLA-4,inhibitors
R13198 T17389 T17390 themeOf GATA3,expression
R13199 T17390 T17388 themeOf expression,inhibitors
R13206 T17396 T17398 causeOf IL-13,inhibit
R13208 T17399 T17401 causeOf TGF-β,induction
R13209 T17400 T17401 themeOf FOXP3,induction
R13210 T17401 T17397 themeOf induction,inhibit
R13211 T17401 T17398 themeOf induction,inhibit
R13213 T17455 T17454 themeOf IL-4,inducing
R13214 T17457 T17458 causeOf TGF-β,mediated
R13215 T17410 T17413 causeOf express,inhibit
R13216 T17411 T17410 themeOf CD25,express
R13217 T17414 T17415 themeOf Foxp3,expression
R13218 T17458 T17456 themeOf mediated,repressed
R13219 T17415 T17413 themeOf expression,inhibit
R13220 T17417 T17416 themeOf drop,contribute
R13221 T17459 T17460 themeOf FOXP3,expression
R13222 T17418 T17419 themeOf Foxp3,expression
R13223 T17419 T17417 themeOf expression,drop
R13224 T17460 T17458 themeOf expression,mediated
R13225 T17421 T17422 themeOf IL-17,producing
R13226 T17466 T17467 themeOf GATA3,expressing
R13227 T17467 T17465 themeOf expressing,high
R13228 T17432 T17433 causeOf IL-4,enhanced
R13229 T17432 T17436 causeOf IL-4,induce
R13230 T17469 T17468 themeOf FOXP3,absence
R13231 T17434 T17435 themeOf FOXP3,expression
R13232 T17435 T17433 themeOf expression,enhanced
R13233 T17437 T17438 themeOf GATA3,expression
R13234 T17470 T17471 causeOf GATA3,inhibited
R13235 T17438 T17436 themeOf expression,induce
R13236 T17472 T17473 themeOf FOXP3,induction
R13237 T17441 T17442 themeOf FOXP3,expression
R13238 T17442 T17440 themeOf expression,regulate
R13239 T17443 T17444 causeOf IL-13,reduce
R13240 T17443 T17446 causeOf IL-13,induce
R13241 T17445 T17444 themeOf FOXP3,reduce
R13242 T17447 T17446 themeOf GATA3,induce
R13243 T17473 T17471 themeOf induction,inhibited
R13244 T17448 T17449 causeOf IL-4,inhibition
R13245 T17449 T17451 themeOf inhibition,mediated
R13246 T17450 T17449 themeOf FOXP3,inhibition
R13247 T17476 T17475 themeOf GATA3,expressing
R13248 T17452 T17451 causeOf GATA3,mediated
R13249 T17453 T17454 causeOf GATA3,inducing
R13250 T17454 T17456 causeOf inducing,repressed
R13251 T17479 T17481 causeOf overexpression,failed
R13252 T17480 T17479 themeOf GATA3,overexpression
R13253 T17482 T17481 themeOf induce,failed
R13254 T17495 T17494 equivalentTo ROG,Repressor of GATA3
R13255 T17498 T17502 themeOf GATA3,binding
R13256 T17483 T17484 themeOf FOXP3,expression
R13257 T17500 T17501 themeOf FOXP3,expression
R13258 T17501 T17499 themeOf expression,repressed
R13259 T17484 T17482 themeOf expression,induce
R13260 T17502 T17499 causeOf binding,repressed
R13261 T17490 T17491 themeOf GATA3,overexpression
R13262 T17504 T17502 themeOf promoter region,binding
R13263 T17504 T17503 partOf promoter region,FOXP3
R13264 T17507 T17506 themeOf GATA3,express
R13265 T17509 T17508 themeOf GATA3,express
R13266 T17511 T17510 themeOf GATA3,bound
R13267 T17536 T17534 themeOf expression,acts as an inhibitor
R13268 T17512 T17513 themeOf GATA3,binds
R13269 T17540 T17537 themeOf promoter,binding
R13270 T17515 T17516 themeOf GATA3,expression
R13271 T17516 T17517 themeOf expression,low
R13272 T17519 T17520 causeOf GATA3,inhibit
R13273 T17540 T17539 partOf promoter,FOXP3
R13274 T17521 T17520 themeOf FOXP3,inhibit
R13275 T17540 T17538 themeOf promoter,repressing
R13276 T17522 T17523 causeOf GATA3,compete
R13277 T17522 T17524 causeOf GATA3,compete
R13278 T17541 T17542 causeOf Il-4,repressing
R13279 T17525 T17523 themeOf binding,compete
R13280 T17543 T17544 themeOf FOXP3,expression
R13281 T17526 T17524 themeOf binding,compete
R13282 T17527 T17525 themeOf AP-1,binding
R13283 T17528 T17526 themeOf NFAT,binding
R13284 T17529 T17525 themeOf promoter,binding
R13285 T17529 T17521 partOf promoter,FOXP3
R13286 T17529 T17526 themeOf promoter,binding
R13287 T17530 T17531 themeOf FOXP3,negatively regulated
R13288 T17544 T17542 themeOf expression,repressing
R13289 T17532 T17531 causeOf IL-4,negatively regulated
R13290 T17533 T17534 causeOf GATA3,acts as an inhibitor
R13291 T17533 T17537 themeOf GATA3,binding
R13292 T17533 T17538 causeOf GATA3,repressing
R13293 T17535 T17536 themeOf FOXP3,expression
R13641 T18038 T18037 themeOf GATA-3,expressing
R13821 T18295 T18296 themeOf IL-4,secretion
R13822 T18296 T18294 themeOf secretion,high
R14366 T19049 T19050 themeOf FOXP3,expression
R14621 T19473 T19474 themeOf CD45RO,depletion
R15284 T20396 T20395 themeOf two zinc fingers,lacking
R15285 T20396 T20394 partOf two zinc fingers,GATA3
R15456 T20694 T20695 themeOf FOXP3,expression
R16208 T21708 T21709 themeOf FOXP3,expression
R16209 T21727 T21728 themeOf Foxp3,expression
R16791 T22540 T22545 themeOf CD3,expression
R16792 T22541 T22544 themeOf CD4,expression
R16793 T22542 T22543 themeOf CD25,expression
R17831 T23766 T23767 themeOf FOXP3,Expression
R17832 T23767 T23765 themeOf Expression,Induce
R18032 T24034 T24033 themeOf FOXP3,Express
R18033 T24035 T24036 themeOf FOXP3,expression
R18034 T24038 T24039 themeOf FOXP3,expression
R18410 T24593 T24594 themeOf IL-4,Producing
R18411 T24596 T24595 themeOf FOXP3,Lack
R18412 T24597 T24599 themeOf FOXP3,expression
R18413 T24598 T24600 themeOf IL-4,expression
R18414 T24602 T24604 themeOf CD45RO,expression
R18415 T24603 T24605 themeOf CD25,expression
R18416 T24613 T24614 themeOf IL-4,secreting
R18417 T24618 T24616 themeOf FOXP3,stained
R18418 T24619 T24617 themeOf IL-4,stained
R18675 T25024 T25025 themeOf FOXP3,Induction
R18676 T25032 T25035 themeOf FOXP3,expression
R18677 T25033 T25034 themeOf GATA3,expression
R18678 T25036 T25038 themeOf GATA3,staining
R18679 T25037 T25039 themeOf FOXP3,staining
R19167 T25762 T25761 causeOf IL-4,Effect
R19168 T25763 T25764 themeOf FOXP3,Induction
R19169 T25764 T25761 themeOf Induction,Effect
R19170 T25771 T25770 themeOf IL-4,increasing
R19171 T25780 T25779 themeOf SMAD2,phosphorylated
R19172 T25781 T25778 themeOf STAT6,phosphorylated
R19173 T25784 T25786 themeOf GATA3,staining
R19174 T25785 T25787 themeOf FOXP3,staining
R19434 T26239 T26241 themeOf GATA3,staining
R19435 T26240 T26242 themeOf FOXP3,staining
R19787 T26816 T26815 themeOf GATA3,presence
R19788 T26820 T26821 themeOf FOXP3,expression
R19789 T26829 T26831 themeOf SMAD7,expression
R19790 T26830 T26832 themeOf TGF-β,expression
R20432 T27678 T27679 causeOf GATA3,Represses
R20433 T27681 T27679 themeOf Promoter,Represses
R20434 T27681 T27680 partOf Promoter,FOXP3
R20435 T27688 T27689 themeOf GATA3,expression
R20436 T27696 T27695 themeOf GATA3,amounts
R20437 T27698 T27699 themeOf GATA3,binding
R20438 T27701 T27699 themeOf promoter,binding
R20439 T27701 T27700 partOf promoter,FOXP3
R599 T768 T769 causeOf transforming growth factor (TGF)-β,mediated
R600 T769 T767 themeOf mediated,deficient
R601 T770 T771 themeOf FOXP3,induction
R602 T771 T769 themeOf induction,mediated
R603 T773 T772 causeOf binding,mediated
R604 T774 T773 themeOf GATA3,binding
R605 T776 T773 themeOf promoter,binding
R606 T776 T775 partOf promoter,FOXP3
R657 T758 T760 themeOf FOXP3,expression
R659 T761 T763 causeOf interleukin 4,inhibits
R661 T764 T763 themeOf FOXP3,inhibits
R662 T766 T765 themeOf GATA-3,overexpressing
R663 T767 T772 themeOf deficient,mediated
R13201 T17391 T17392 causeOf GATA3,inhibits
R13202 T17393 T17394 themeOf FOXP3,expression
R13204 T17394 T17392 themeOf expression,inhibits
R13205 T17395 T17397 causeOf IL-4,inhibit

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T640 0-26 Protein denotes GATA3-Driven Th2 Responses
T641 35-49 Protein denotes TGF-β1–Induced
T642 50-55 Protein denotes FOXP3
T643 56-66 Gene_expression denotes Expression
T644 56-66 Gene_expression denotes Expression
T645 27-34 Negative_regulation denotes Inhibit
T646 27-34 Negative_regulation denotes Inhibit
T647 364-367 Protein denotes Th1
T648 372-386 Protein denotes Th2 phenotypes
T649 388-393 Protein denotes FOXP3
T650 545-555 Gene_expression denotes expression
T651 530-540 Regulation denotes regulating
T652 708-721 Protein denotes interleukin 4
T653 723-727 Protein denotes IL-4
T654 776-781 Protein denotes FOXP3
T655 767-775 Negative_regulation denotes inhibits
T656 767-775 Negative_regulation denotes inhibits
T657 890-896 Protein denotes GATA-3
T658 988-993 Protein denotes FOXP3
T659 875-889 Gene_expression denotes overexpressing
T660 994-1003 Positive_regulation denotes induction
T661 931-940 Negative_regulation denotes deficient
T662 1054-1059 Protein denotes GATA3
T663 1067-1081 Protein denotes FOXP3 promoter
T664 1043-1050 Binding denotes binding
T665 1043-1050 Binding denotes binding
T666 1245-1249 Protein denotes IL-4
T667 1344-1355 Protein denotes target IL-4
T668 1359-1364 Protein denotes GATA3
T2298 3196-3199 Protein denotes Th1
T2299 3204-3207 Protein denotes Th2
T2300 3423-3426 Protein denotes Th2
T2301 3496-3509 Protein denotes interleukin 4
T2302 3511-3515 Protein denotes IL-4
T2303 3525-3530 Protein denotes STAT6
T2304 3535-3540 Protein denotes GATA3
T2305 3516-3524 Positive_regulation denotes –induced
T2306 3516-3524 Positive_regulation denotes –induced
T2307 3516-3524 Positive_regulation denotes –induced
T2308 3516-3524 Positive_regulation denotes –induced
T2309 3620-3625 Protein denotes GATA3
T2310 3713-3717 Protein denotes IL-4
T2311 3719-3723 Protein denotes IL-5
T2312 3728-3733 Protein denotes IL-13
T2313 3789-3792 Protein denotes Th1
T2314 3700-3709 Positive_regulation denotes induction
T2315 3700-3709 Positive_regulation denotes induction
T2316 3700-3709 Positive_regulation denotes induction
T2317 3775-3785 Negative_regulation denotes inhibition
T2318 3873-3878 Protein denotes GATA3
T2319 3863-3872 Regulation denotes modulates
T2320 4077-4082 Protein denotes GATA3
T2321 4297-4302 Protein denotes GATA3
T2322 4344-4349 Protein denotes GATA3
T2323 4350-4358 Negative_regulation denotes blockade
T2324 4768-4803 Protein denotes forkhead transcription factor FOXP3
T2325 4831-4835 Protein denotes CD25
T2326 4882-4885 Protein denotes Th1
T2327 4889-4902 Protein denotes Th2 cytokines
T2328 4756-4763 Gene_expression denotes express
T2329 4823-4830 Gene_expression denotes express
T2330 4868-4878 Gene_expression denotes expression
T2331 4868-4878 Gene_expression denotes expression
T2332 4863-4867 Negative_regulation denotes lack
T2333 4863-4867 Negative_regulation denotes lack
T2334 5106-5111 Protein denotes FOXP3
T2335 5098-5105 Gene_expression denotes express
T2336 5176-5191 Protein denotes T cell receptor
T2337 5193-5196 Protein denotes TCR
T2338 5234-5239 Protein denotes FOXP3
T2339 5240-5250 Gene_expression denotes expression
T2340 5227-5233 Positive_regulation denotes induce
T2341 5356-5361 Protein denotes TGF-β
T2342 5492-5500 Protein denotes TGF-βRII
T2343 5480-5487 Negative_regulation denotes lacking
T2344 5649-5654 Protein denotes TGF-β
T2345 5833-5838 Protein denotes GATA3
T2346 5843-5848 Protein denotes FOXP3
T2347 5911-5916 Protein denotes GATA3
T2348 5921-5924 Protein denotes Th1
T2349 5977-5982 Protein denotes GATA3
T2350 5992-5997 Protein denotes FOXP3
T2351 6029-6034 Protein denotes FOXP3
T2352 6051-6056 Protein denotes GATA3
T2353 5998-6007 Positive_regulation denotes induction
T2354 6035-6045 Gene_expression denotes expression
T2355 5983-5991 Negative_regulation denotes inhibits
T2356 6022-6028 Negative_regulation denotes limits
T2357 6116-6121 Protein denotes GATA3
T2358 6144-6158 Protein denotes FOXP3 promoter
T2359 6131-6136 Binding denotes binds
T5943 11403-11408 Protein denotes FOXP3
T5944 11413-11418 Protein denotes GATA3
T5945 11517-11522 Protein denotes FOXP3
T5946 11523-11533 Gene_expression denotes expression
T5947 11510-11516 Positive_regulation denotes induce
T5948 11665-11670 Protein denotes FOXP3
T5949 11675-11680 Protein denotes GATA3
T5950 11717-11722 Protein denotes CD4 T
T5951 11748-11757 Protein denotes anti-IL-4
T5952 11759-11769 Protein denotes anti-IFN-γ
T5953 11651-11661 Gene_expression denotes expression
T5954 11651-11661 Gene_expression denotes expression
T5955 11864-11869 Protein denotes FOXP3
T5956 11874-11879 Protein denotes GATA3
T5957 11897-11907 Gene_expression denotes expression
T5958 11897-11907 Gene_expression denotes expression
T5959 12039-12049 Protein denotes FOXP3 mRNA
T5960 12050-12060 Gene_expression denotes expression
T5961 12061-12070 Positive_regulation denotes increased
T5962 12127-12132 Protein denotes GATA3
T5963 12137-12142 Protein denotes FOXP3
T5964 12174-12184 Gene_expression denotes expression
T5965 12174-12184 Gene_expression denotes expression
T5966 12373-12378 Protein denotes FOXP3
T5967 12427-12432 Protein denotes GATA3
T5968 12357-12367 Gene_expression denotes expressing
T5969 12403-12412 Gene_expression denotes expressed
T5970 12698-12706 Protein denotes anti-CD3
T5971 12736-12741 Protein denotes CD103
T5972 12743-12749 Protein denotes CTLA-4
T5973 12751-12755 Protein denotes GITR
T5974 12894-12899 Protein denotes GATA3
T5975 12904-12909 Protein denotes FOXP3
T5976 12910-12920 Gene_expression denotes expression
T5977 12910-12920 Gene_expression denotes expression
T5978 13110-13115 Protein denotes FOXP3
T5979 13132-13135 Protein denotes Th2
T5980 13102-13109 Gene_expression denotes express
T5981 13079-13083 Negative_regulation denotes lost
T10850 20242-20247 Protein denotes GATA3
T10851 20275-20280 Protein denotes FOXP3
T10852 20281-20291 Gene_expression denotes Expression
T10853 20262-20271 Negative_regulation denotes Regulator
T10854 20292-20297 Protein denotes FOXP3
T10855 20324-20329 Protein denotes GATA3
T10856 20398-20403 Protein denotes GATA3
T10857 20418-20423 Protein denotes FOXP3
T10858 20298-20308 Gene_expression denotes expression
T10859 20309-20318 Negative_regulation denotes decreased
T10860 20330-20340 Gene_expression denotes expression
T10861 20344-20348 Positive_regulation denotes high
T10862 20407-20417 Negative_regulation denotes repressing
T10863 20433-20438 Protein denotes GATA3
T10864 20488-20493 Protein denotes GATA3
T10865 20583-20588 Protein denotes STAT4
T10866 20622-20627 Protein denotes GATA3
T10867 20589-20599 Gene_expression denotes expression
T10868 20572-20582 Negative_regulation denotes inhibiting
T10869 20692-20697 Protein denotes GATA3
T10870 20719-20724 Protein denotes FOXP3
T10871 20750-20755 Protein denotes GATA3
T10872 20771-20776 Protein denotes GATA3
T10873 20825-20829 Protein denotes CD4+
T10874 20888-20893 Protein denotes GATA3
T10875 20725-20734 Positive_regulation denotes induction
T10876 20801-20807 Entity denotes domain
T10877 20878-20887 Gene_expression denotes expressed
T10878 20711-20718 Negative_regulation denotes inhibit
T10879 20777-20784 Negative_regulation denotes lacking
T10880 20958-20961 Protein denotes CD3
T10881 20962-20966 Protein denotes CD28
T10882 20997-21002 Protein denotes TGF-β
T10883 21004-21007 Protein denotes TAT
T10884 21008-21013 Protein denotes GATA3
T10885 21135-21138 Protein denotes TAT
T10886 21139-21144 Protein denotes GATA3
T10887 21153-21158 Protein denotes FOXP3
T10888 21246-21249 Protein denotes TAT
T10889 21250-21254 Protein denotes ΔDBD
T10890 21255-21260 Protein denotes GATA3
T10891 21277-21282 Protein denotes FOXP3
T10892 21159-21169 Gene_expression denotes expression
T10893 21283-21293 Gene_expression denotes expression
T10894 21145-21152 Negative_regulation denotes reduced
T10895 21145-21152 Negative_regulation denotes reduced
T10896 21270-21276 Regulation denotes affect
T10897 21407-21412 Protein denotes GATA3
T10898 21416-21421 Protein denotes FOXP3
T10899 21480-21485 Protein denotes GATA3
T10900 21511-21520 Protein denotes CD2 locus
T10901 21465-21479 Positive_regulation denotes overexpressing
T10902 21587-21590 Protein denotes CD4
T10903 21620-21624 Protein denotes DO11
T10904 21625-21631 Protein denotes 10xCD2
T10905 21632-21637 Protein denotes GATA3
T10906 21803-21808 Protein denotes GATA3
T10907 21812-21817 Protein denotes iTreg
T10908 21819-21822 Protein denotes CD4
T10909 21823-21828 Protein denotes CD62L
T10910 21829-21833 Protein denotes CD25
T10911 21905-21910 Protein denotes TGF-β
T10912 21916-21921 Protein denotes Foxp3
T10913 21922-21932 Gene_expression denotes expression
T10914 21967-21970 Protein denotes CD4
T10915 21971-21975 Protein denotes CD25
T10916 21988-21993 Protein denotes Foxp3
T10917 22049-22054 Protein denotes TGF-β
T10918 22081-22086 Protein denotes Foxp3
T10919 22068-22080 Positive_regulation denotes up-regulated
T10920 22155-22158 Protein denotes CD2
T10921 22159-22169 Protein denotes GATA3xDO11
T10922 22206-22211 Protein denotes Foxp3
T10923 22243-22248 Protein denotes TGF-β
T10924 22212-22222 Gene_expression denotes expression
T10925 22198-22205 Negative_regulation denotes reduced
T10926 22307-22312 Protein denotes TGF-β
T10927 22327-22332 Protein denotes Smad7
T10928 22423-22428 Protein denotes TGF-β
T10929 22279-22287 Gene_expression denotes produced
T10930 22345-22354 Gene_expression denotes expressed
T10931 23382-23386 Protein denotes IL-4
T10932 23395-23421 Protein denotes GATA3 transcription factor
T10933 23387-23394 Positive_regulation denotes induced
T12645 24407-24421 Positive_regulation denotes Overexpression
T12646 24470-24478 Positive_regulation denotes activity
T12647 24470-24478 Positive_regulation denotes activity
T12648 24448-24458 Negative_regulation denotes diminished
T12649 24448-24458 Negative_regulation denotes diminished
T12650 24586-24596 Protein denotes GATA3 site
T12651 24648-24666 Protein denotes GATA3-binding site
T12652 24692-24702 Protein denotes luciferase
T12653 24732-24735 Protein denotes CD4
T12654 24736-24742 Protein denotes CD45RO
T12655 24806-24809 Protein denotes CD4
T12656 24810-24816 Protein denotes CD45RA
T12657 24861-24866 Protein denotes GATA3
T12658 24874-24888 Protein denotes FOXP3 promoter
T12659 24682-24691 Positive_regulation denotes increased
T12660 24914-24919 Protein denotes GATA3
T12661 24942-24956 Protein denotes FOXP3 promoter
T12662 24920-24925 Binding denotes binds
T12663 24920-24925 Binding denotes binds
T12664 25037-25042 Protein denotes GATA3
T12665 25150-25160 Protein denotes GATA3 site
T12666 25168-25182 Protein denotes FOXP3 promoter
T12667 25227-25245 Protein denotes GATA3-binding site
T12668 25135-25145 Negative_regulation denotes containing
T12669 25268-25273 Protein denotes GATA3
T12670 25274-25281 Binding denotes binding
T12671 25323-25328 Protein denotes GATA3
T12672 25329-25339 Gene_expression denotes expressing
T12673 25429-25434 Protein denotes GATA3
T12674 25456-25461 Protein denotes GATA3
T12675 25414-25428 Gene_expression denotes overexpressing
T12676 25462-25469 Binding denotes binding
T12677 25535-25540 Protein denotes GATA3
T12678 25563-25577 Protein denotes FOXP3 promoter
T12679 25541-25546 Binding denotes binds
T12680 25541-25546 Binding denotes binds
T12681 25685-25704 Protein denotes anti-GATA3 antibody
T12682 25721-25726 Protein denotes GATA3
T12683 25740-25754 Protein denotes FOXP3 promoter
T12684 25755-25761 Entity denotes region
T12685 25727-25732 Binding denotes binds
T12686 25859-25864 Protein denotes GATA3
T12687 25880-25894 Protein denotes FOXP3 promoter
T12688 25909-25914 Protein denotes FOXP3
T12689 25865-25872 Binding denotes binding
T12690 25915-25925 Gene_expression denotes expression
T12691 25898-25908 Negative_regulation denotes repressing
T18010 35419-35449 Protein denotes human CD2 locus control region
T18011 35451-35454 Protein denotes CD2
T18012 35455-35460 Protein denotes GATA3
T18013 35481-35498 Protein denotes DO11.10xCD2-GATA3
T18014 35386-35396 Gene_expression denotes expressing
T18015 35386-35396 Gene_expression denotes expressing
T18711 36303-36310 Protein denotes GenBank
T18712 36389-36394 Protein denotes FOXP3
T18713 36417-36432 Protein denotes ACA GCA CAT TCC
T18714 36433-36440 Protein denotes CAG AGT
T18715 36448-36453 Protein denotes FOXP3
T18716 36499-36504 Protein denotes EF-1α
T18717 36558-36563 Protein denotes GATA3
T18718 36783-36792 Protein denotes ABI PRISM
T18719 36977-36988 Protein denotes Ubiquitin C
T18720 37141-37147 Protein denotes TGF-ß1
T18721 37568-37579 Protein denotes ubiquitin C
T19414 38323-38326 Protein denotes CD4
T19415 38328-38335 Protein denotes CD45RA+
T19416 38357-38363 Protein denotes CD45RO
T19417 38498-38502 Protein denotes Okt3
T19418 38504-38508 Protein denotes IgG1
T19419 38537-38540 Protein denotes Th1
T19420 38644-38654 Protein denotes anti-IFN-γ
T19421 38737-38747 Protein denotes anti-IFN-γ
T19422 38364-38373 Negative_regulation denotes depletion
T19714 38876-38890 Protein denotes FOXP3 promoter
T19715 38934-38948 Protein denotes FOXP3 promoter
T19716 39021-39025 Protein denotes pGL3
T19717 39026-39031 Protein denotes FOXP3
T19718 39081-39095 Protein denotes FOXP3 promoter
T19719 39266-39281 Protein denotes GTT TCT CAT GAG
T19720 39282-39332 Protein denotes CCC TAT TAA GTC ATT CTT ACC TCT CAC CTC TGT GGT GA
T20641 40747-40750 Protein denotes TAT
T20642 40751-40756 Protein denotes GATA3
T20643 40771-40774 Protein denotes CD4
T20644 40775-40781 Protein denotes CD45RA
T20645 40890-40895 Protein denotes GATA3
T20646 40980-40988 Protein denotes anti-CD3
T20647 40993-41002 Protein denotes anti-CD28
T20648 41007-41012 Protein denotes TGF-β
T20649 41039-41051 Protein denotes TAT proteins
T20650 41086-41091 Protein denotes FOXP3
T20651 41092-41102 Gene_expression denotes expression
T21619 41610-41615 Protein denotes FOXP3
T21620 41616-41626 Gene_expression denotes expression
T21621 42220-42225 Protein denotes CD62L
T21622 42310-42335 Protein denotes ECD-conjugated mouse IgG1
T21623 42339-42348 Protein denotes rat IgG2a
T21624 42483-42486 Protein denotes CD3
T21625 42493-42496 Protein denotes CD4
T21626 42507-42511 Protein denotes CD25
T21627 42513-42537 Protein denotes Anti-FcγRII/III antibody
T21628 42646-42651 Protein denotes CD4 T
T21629 42753-42758 Protein denotes CD62L
T21630 42759-42761 Protein denotes PE
T21631 42772-42775 Protein denotes CD4
T21632 42776-42779 Protein denotes APC
T21633 42885-42890 Protein denotes Foxp3
T21634 43050-43053 Protein denotes CD4
T21635 43054-43057 Protein denotes APC
T21636 43062-43066 Protein denotes LIVE
T21637 43067-43071 Protein denotes DEAD
T21638 42891-42901 Gene_expression denotes expression
T22162 43267-43272 Protein denotes FOXP3
T22163 43312-43315 Protein denotes CD4
T22164 43316-43320 Protein denotes CD25
T22165 43641-43656 Protein denotes goat anti-FOXP3
T22166 43759-43762 Protein denotes mAb
T22167 44112-44122 Protein denotes anti-STAT6
T22499 44330-44361 Protein denotes anti-IL-4 mAb (11B11 or MAB404)
T22500 44521-44524 Protein denotes CD3
T22501 44526-44529 Protein denotes CD4
T22502 44535-44539 Protein denotes CD25
T22503 44540-44550 Gene_expression denotes expression
T22504 44540-44550 Gene_expression denotes expression
T22505 44540-44550 Gene_expression denotes expression
T22506 44556-44582 Protein denotes anti-mouse IL-4 mAb MAB404
T22507 44625-44650 Protein denotes anti-mouse IL-4 mAb 11B11
T22949 45072-45075 Protein denotes IgG
T23721 7478-7486 Protein denotes anti-CD3
T23722 7487-7491 Protein denotes CD28
T23723 7508-7513 Protein denotes TGF-β
T23724 7587-7597 Protein denotes FOXP3 mRNA
T23725 7758-7766 Protein denotes anti-CD3
T23726 7767-7771 Protein denotes CD28
T23727 7773-7778 Protein denotes TGF-β
T23728 7783-7792 Protein denotes anti-IL-4
T24006 10231-10234 Protein denotes Th2
T24007 10342-10347 Protein denotes TGF-β
T24008 10212-10222 Gene_expression denotes expression
T24009 10212-10222 Gene_expression denotes expression
T24010 10212-10222 Gene_expression denotes expression
T24011 10349-10354 Protein denotes FOXP3
T24012 10355-10365 Gene_expression denotes expression
T24506 10699-10704 Protein denotes FOXP3
T24507 10709-10713 Protein denotes IL-4
T24508 10714-10724 Gene_expression denotes expression
T24509 10714-10724 Gene_expression denotes expression
T24510 10762-10766 Protein denotes CD4+
T24511 10802-10808 Protein denotes CD45RO
T24512 10813-10825 Protein denotes CD25 surface
T24513 10892-10898 Protein denotes CD45RO
T24514 10899-10903 Protein denotes CD25
T24515 10962-10968 Protein denotes CD45RO
T24516 10969-10973 Protein denotes CD25
T24517 10826-10836 Gene_expression denotes expression
T24518 10826-10836 Gene_expression denotes expression
T24519 11328-11333 Protein denotes FOXP3
T24520 11338-11342 Protein denotes IL-4
T24521 11316-11323 Gene_expression denotes stained
T24522 11316-11323 Gene_expression denotes stained
T24958 13213-13218 Protein denotes FOXP3
T24959 13219-13228 Positive_regulation denotes Induction
T24960 13268-13278 Protein denotes Human CD4+
T24961 13331-13334 Protein denotes CD3
T24962 13335-13339 Protein denotes CD28
T24963 13359-13364 Protein denotes TGF-β
T24964 13491-13496 Protein denotes FOXP3
T24965 13501-13506 Protein denotes GATA3
T24966 13507-13517 Gene_expression denotes expression
T24967 13507-13517 Gene_expression denotes expression
T24968 13596-13601 Protein denotes GATA3
T24969 13606-13611 Protein denotes FOXP3
T24970 13648-13651 Protein denotes CD4
T24971 13652-13658 Protein denotes CD45RA
T24972 13612-13620 Gene_expression denotes staining
T24973 13612-13620 Gene_expression denotes staining
T25646 16508-16512 Protein denotes IL-4
T25647 16516-16521 Protein denotes FOXP3
T25648 16522-16531 Positive_regulation denotes Induction
T25649 16498-16504 Regulation denotes Effect
T25650 16636-16640 Protein denotes IL-4
T25651 16869-16872 Protein denotes CD4
T25652 16873-16879 Protein denotes CD45RA
T25653 16949-16954 Protein denotes TGF-β
T25654 17001-17005 Protein denotes IL-4
T25655 16973-16983 Positive_regulation denotes increasing
T25656 17081-17084 Protein denotes CD4
T25657 17085-17091 Protein denotes CD45RA
T25658 17146-17149 Protein denotes CD3
T25659 17150-17154 Protein denotes CD28
T25660 17156-17161 Protein denotes TGF-β
T25661 17177-17181 Protein denotes IL-4
T25662 17294-17299 Protein denotes SMAD2
T25663 17304-17309 Protein denotes STAT6
T25664 17279-17293 Phosphorylation denotes phosphorylated
T25665 17279-17293 Phosphorylation denotes phosphorylated
T25666 17317-17322 Protein denotes STAT6
T25667 17327-17332 Protein denotes GAPDH
T25668 17379-17384 Protein denotes GATA3
T25669 17389-17394 Protein denotes FOXP3
T25670 17432-17435 Protein denotes CD4
T25671 17436-17442 Protein denotes CD45RA
T25672 17455-17459 Protein denotes IL-4
T25673 17395-17403 Gene_expression denotes staining
T25674 17395-17403 Gene_expression denotes staining
T26162 18665-18669 Protein denotes IL-4
T26163 18679-18710 Protein denotes TGF-β–Mediated iTreg Commitment
T26164 18670-18678 Negative_regulation denotes Inhibits
T26165 18724-18727 Protein denotes CD4
T26166 18728-18734 Protein denotes CD45RA
T26167 18779-18782 Protein denotes CD3
T26168 18783-18787 Protein denotes CD28
T26169 18789-18794 Protein denotes TGF-β
T26170 18800-18804 Protein denotes IL-4
T26171 19101-19106 Protein denotes GATA3
T26172 19111-19116 Protein denotes FOXP3
T26173 19168-19171 Protein denotes CD4
T26174 19172-19178 Protein denotes CD45RA
T26175 19204-19208 Protein denotes IL-4
T26176 19213-19218 Protein denotes TGF-β
T26177 19117-19125 Gene_expression denotes staining
T26178 19117-19125 Gene_expression denotes staining
T26723 22450-22455 Protein denotes GATA3
T26724 22488-22493 Protein denotes FOXP3
T26725 22494-22504 Gene_expression denotes Expression
T26726 22521-22525 Protein denotes CD4+
T26727 22586-22589 Protein denotes TAT
T26728 22590-22595 Protein denotes GATA3
T26729 22635-22640 Protein denotes GATA3
T26730 22680-22683 Protein denotes CD3
T26731 22684-22688 Protein denotes CD28
T26732 22623-22631 Negative_regulation denotes presence
T26733 22775-22780 Protein denotes FOXP3
T26734 22781-22791 Gene_expression denotes expression
T26735 22902-22905 Protein denotes CD4
T26736 22906-22910 Protein denotes CD25
T26737 22999-23004 Protein denotes TGF-β
T26738 23023-23026 Protein denotes CD4
T26739 23230-23235 Protein denotes SMAD7
T26740 23240-23245 Protein denotes TGF-β
T26741 23246-23256 Gene_expression denotes expression
T26742 23246-23256 Gene_expression denotes expression
T27583 25937-25942 Protein denotes GATA3
T27584 25957-25977 Protein denotes Human FOXP3 Promoter
T27585 25943-25952 Negative_regulation denotes Represses
T27586 26019-26022 Protein denotes CD4
T27587 26116-26130 Protein denotes FOXP3 promoter
T27588 26151-26175 Protein denotes luciferase reporter gene
T27589 26241-26259 Protein denotes renilla luciferase
T27590 26341-26346 Protein denotes CD4 T
T27591 26379-26393 Protein denotes FOXP3 promoter
T27592 26429-26434 Protein denotes GATA3
T27593 26435-26445 Gene_expression denotes expression
T27594 26578-26583 Protein denotes CD4 T
T27595 26627-26678 Protein denotes GATA3 mutated 511-FOXP3 promoter reporter construct
T27596 26781-26799 Protein denotes renilla luciferase
T27597 26939-26944 Protein denotes GATA3
T27598 27165-27178 Protein denotes GATA3 protein
T27599 27236-27250 Protein denotes anti-GATA3 mAb
T27600 27154-27161 Gene_expression denotes amounts
T27601 27398-27402 Protein denotes ChIP
T27602 27407-27412 Protein denotes GATA3
T27603 27428-27442 Protein denotes FOXP3 promoter
T27604 27413-27420 Binding denotes binding
T27605 27413-27420 Binding denotes binding
T12623 23457-23462 Protein denotes GATA3
T12624 23477-23491 Protein denotes FOXP3 Promoter
T12625 23463-23472 Negative_regulation denotes Represses
T12626 23534-23539 Protein denotes GATA3
T12627 23563-23574 Protein denotes human FOXP3
T12628 23580-23600 Protein denotes human FOXP3 promoter
T12629 23648-23653 Protein denotes GATA3
T12630 23549-23559 Negative_regulation denotes repression
T12631 23882-23887 Protein denotes FOXP3
T12632 23888-23898 Regulation denotes regulation
T12633 23874-23878 Regulation denotes role
T12634 23958-23963 Protein denotes FOXP3
T12635 24018-24022 Protein denotes CD4+
T12636 24166-24180 Protein denotes FOXP3 promoter
T12637 24226-24231 Protein denotes GATA3
T12638 24345-24348 Protein denotes CD4
T12639 24388-24393 Protein denotes GATA3
T12640 24232-24242 Gene_expression denotes expressing
T12641 24362-24369 Gene_expression denotes express
T12642 24425-24430 Protein denotes GATA3
T12643 24459-24469 Protein denotes luciferase
T12644 24486-24500 Protein denotes FOXP3 promoter
T20369 40386-40393 Entity denotes fingers
T18262 35795-35799 Protein denotes CD4+
T18263 35809-35813 Protein denotes CD4+
T18264 35979-35983 Protein denotes CD4+
T18265 36127-36133 Protein denotes Vbeta8
T18266 36134-36142 Gene_expression denotes positive
T18267 36182-36186 Protein denotes IL-4
T18268 36187-36196 Localization denotes secretion
T19016 37820-37824 Protein denotes CD4+
T19017 37834-37837 Protein denotes CD4
T19018 37840-37845 Protein denotes CD62L
T19019 37852-37857 Protein denotes CD25–
T19020 38118-38126 Protein denotes rhTGF-ß1
T19021 38221-38226 Protein denotes FOXP3
T19022 38227-38237 Gene_expression denotes expression
T20008 39467-39501 Protein denotes FOXP3 promoter luciferase reporter
T20009 39779-39789 Protein denotes luciferase
T20010 39824-39834 Protein denotes luciferase
T20011 39952-39970 Protein denotes renilla luciferase
T20149 40159-40165 Protein denotes TaqMan
T20365 40282-40306 Protein denotes Recombinant TAT proteins
T20366 40327-40340 Protein denotes GATA3 protein
T20367 40358-40363 Protein denotes GATA3
T20368 40457-40469 Protein denotes TAT sequence
T20370 40365-40372 Negative_regulation denotes lacking
T20371 40631-40634 Protein denotes TAT
T22950 45490-45504 Protein denotes FOXP3 promoter
T23415 45716-45720 Protein denotes CD4+
T23416 45899-45926 Protein denotes mM DTT, protease inhibitors
T23417 45928-45933 Protein denotes Sigma
T24004 10206-10211 Protein denotes FOXP3
T24005 10226-10229 Protein denotes Th1
R539 T640 T645 causeOf GATA3-Driven Th2 Responses,Inhibit
R540 T640 T646 causeOf GATA3-Driven Th2 Responses,Inhibit
R541 T641 T643 themeOf TGF-β1–Induced,Expression
R542 T642 T644 themeOf FOXP3,Expression
R543 T643 T645 themeOf Expression,Inhibit
R555 T662 T665 themeOf GATA3,binding
R556 T663 T665 themeOf FOXP3 promoter,binding
R1995 T2301 T2305 causeOf interleukin 4,–induced
R1996 T2301 T2306 causeOf interleukin 4,–induced
R1998 T2302 T2307 causeOf IL-4,–induced
R1999 T2302 T2308 causeOf IL-4,–induced
R2000 T2303 T2305 themeOf STAT6,–induced
R2001 T2303 T2307 themeOf STAT6,–induced
R2002 T2304 T2306 themeOf GATA3,–induced
R2003 T2304 T2308 themeOf GATA3,–induced
R2005 T2309 T2314 causeOf GATA3,induction
R2006 T2309 T2315 causeOf GATA3,induction
R2007 T2309 T2316 causeOf GATA3,induction
R2008 T2309 T2317 causeOf GATA3,inhibition
R2009 T2310 T2314 themeOf IL-4,induction
R2011 T2311 T2315 themeOf IL-5,induction
R2012 T2312 T2316 themeOf IL-13,induction
R2013 T2313 T2317 themeOf Th1,inhibition
R2017 T2318 T2319 themeOf GATA3,modulates
R2019 T2322 T2323 themeOf GATA3,blockade
R2020 T2324 T2328 themeOf forkhead transcription factor FOXP3,express
R2021 T2325 T2329 themeOf CD25,express
R2022 T2326 T2330 themeOf Th1,expression
R2023 T2327 T2331 themeOf Th2 cytokines,expression
R2025 T2330 T2332 themeOf expression,lack
R2026 T2331 T2333 themeOf expression,lack
R2028 T2334 T2335 themeOf FOXP3,express
R2030 T2338 T2339 themeOf FOXP3,expression
R2033 T2339 T2340 themeOf expression,induce
R2035 T2342 T2343 themeOf TGF-βRII,lacking
R2038 T2349 T2355 causeOf GATA3,inhibits
R2040 T2350 T2353 themeOf FOXP3,induction
R2041 T2351 T2354 themeOf FOXP3,expression
R2042 T2353 T2355 themeOf induction,inhibits
R2043 T2354 T2356 themeOf expression,limits
R2045 T2357 T2359 themeOf GATA3,binds
R2046 T2358 T2359 themeOf FOXP3 promoter,binds
R4654 T5945 T5946 themeOf FOXP3,expression
R4655 T5946 T5947 themeOf expression,induce
R4656 T5948 T5953 themeOf FOXP3,expression
R4657 T5949 T5954 themeOf GATA3,expression
R4658 T5955 T5957 themeOf FOXP3,expression
R4659 T5956 T5958 themeOf GATA3,expression
R4660 T5959 T5960 themeOf FOXP3 mRNA,expression
R4661 T5960 T5961 themeOf expression,increased
R4662 T5962 T5964 themeOf GATA3,expression
R4663 T5963 T5965 themeOf FOXP3,expression
R4664 T5966 T5968 themeOf FOXP3,expressing
R4665 T5967 T5969 themeOf GATA3,expressed
R4666 T5974 T5976 themeOf GATA3,expression
R4667 T5975 T5977 themeOf FOXP3,expression
R4668 T5978 T5980 themeOf FOXP3,express
R4669 T5980 T5981 themeOf express,lost
R8262 T10850 T10853 causeOf GATA3,Regulator
R8263 T10851 T10852 themeOf FOXP3,Expression
R8264 T10852 T10853 themeOf Expression,Regulator
R8265 T10854 T10858 themeOf FOXP3,expression
R8266 T10855 T10860 themeOf GATA3,expression
R8267 T10857 T10862 themeOf FOXP3,repressing
R8268 T10858 T10859 themeOf expression,decreased
R8269 T10860 T10861 themeOf expression,high
R8270 T10865 T10867 themeOf STAT4,expression
R8271 T10867 T10868 themeOf expression,inhibiting
R8272 T10869 T10878 causeOf GATA3,inhibit
R8273 T10870 T10875 themeOf FOXP3,induction
R8274 T10872 T10879 themeOf GATA3,lacking
R8275 T10874 T10877 themeOf GATA3,expressed
R8276 T10875 T10878 themeOf induction,inhibit
R8277 T10876 T10879 Site domain,lacking
R8278 T10885 T10894 causeOf TAT,reduced
R8279 T10886 T10895 causeOf GATA3,reduced
R8280 T10887 T10892 themeOf FOXP3,expression
R8281 T10891 T10893 themeOf FOXP3,expression
R8282 T10892 T10894 themeOf expression,reduced
R8283 T10892 T10895 themeOf expression,reduced
R8284 T10893 T10896 themeOf expression,affect
R8285 T10899 T10901 themeOf GATA3,overexpressing
R8286 T10912 T10913 themeOf Foxp3,expression
R8287 T10917 T10919 causeOf TGF-β,up-regulated
R8288 T10918 T10919 themeOf Foxp3,up-regulated
R8289 T10922 T10924 themeOf Foxp3,expression
R8290 T10924 T10925 themeOf expression,reduced
R8291 T10926 T10929 themeOf TGF-β,produced
R8292 T10927 T10930 themeOf Smad7,expressed
R8293 T10931 T10933 causeOf IL-4,induced
R8294 T10932 T10933 themeOf GATA3 transcription factor,induced
R9543 T12677 T12679 themeOf GATA3,binds
R9544 T12677 T12680 themeOf GATA3,binds
R9545 T12678 T12680 themeOf FOXP3 promoter,binds
R9546 T12682 T12685 themeOf GATA3,binds
R9547 T12683 T12685 themeOf FOXP3 promoter,binds
R9548 T12683 T12684 partOf FOXP3 promoter,region
R9549 T12684 T12685 Site region,binds
R9550 T12686 T12689 themeOf GATA3,binding
R9551 T12687 T12689 themeOf FOXP3 promoter,binding
R9552 T12688 T12690 themeOf FOXP3,expression
R9553 T12689 T12691 causeOf binding,repressing
R9554 T12690 T12691 themeOf expression,repressing
R13635 T18010 T18014 themeOf human CD2 locus control region,expressing
R13636 T18012 T18015 themeOf GATA3,expressing
R13813 T18265 T18266 themeOf Vbeta8,positive
R13814 T18267 T18268 themeOf IL-4,secretion
R14362 T19021 T19022 themeOf FOXP3,expression
R14617 T19416 T19422 themeOf CD45RO,depletion
R15277 T20367 T20370 themeOf GATA3,lacking
R15278 T20367 T20369 partOf GATA3,fingers
R15279 T20369 T20370 Site fingers,lacking
R15452 T20650 T20651 themeOf FOXP3,expression
R16200 T21619 T21620 themeOf FOXP3,expression
R16201 T21633 T21638 themeOf Foxp3,expression
R16780 T22500 T22503 themeOf CD3,expression
R16781 T22501 T22504 themeOf CD4,expression
R16782 T22502 T22505 themeOf CD25,expression
R18019 T24004 T24008 themeOf FOXP3,expression
R18020 T24005 T24009 themeOf Th1,expression
R18021 T24006 T24010 themeOf Th2,expression
R18022 T24011 T24012 themeOf FOXP3,expression
R18383 T24506 T24508 themeOf FOXP3,expression
R18384 T24507 T24509 themeOf IL-4,expression
R18385 T24511 T24517 themeOf CD45RO,expression
R18386 T24512 T24518 themeOf CD25 surface,expression
R18387 T24519 T24521 themeOf FOXP3,stained
R18388 T24520 T24522 themeOf IL-4,stained
R18656 T24958 T24959 themeOf FOXP3,Induction
R18657 T24964 T24966 themeOf FOXP3,expression
R18658 T24965 T24967 themeOf GATA3,expression
R18659 T24968 T24972 themeOf GATA3,staining
R18660 T24969 T24973 themeOf FOXP3,staining
R19135 T25646 T25649 causeOf IL-4,Effect
R19136 T25647 T25648 themeOf FOXP3,Induction
R19137 T25648 T25649 themeOf Induction,Effect
R19138 T25654 T25655 themeOf IL-4,increasing
R19139 T25662 T25664 themeOf SMAD2,phosphorylated
R19140 T25663 T25665 themeOf STAT6,phosphorylated
R19141 T25668 T25673 themeOf GATA3,staining
R19142 T25669 T25674 themeOf FOXP3,staining
R19423 T26162 T26164 causeOf IL-4,Inhibits
R19424 T26163 T26164 themeOf TGF-β–Mediated iTreg Commitment,Inhibits
R19425 T26171 T26177 themeOf GATA3,staining
R19426 T26172 T26178 themeOf FOXP3,staining
R19771 T26724 T26725 themeOf FOXP3,Expression
R19772 T26729 T26732 themeOf GATA3,presence
R19773 T26733 T26734 themeOf FOXP3,expression
R19774 T26739 T26741 themeOf SMAD7,expression
R19775 T26740 T26742 themeOf TGF-β,expression
R20402 T27583 T27585 causeOf GATA3,Represses
R20403 T27584 T27585 themeOf Human FOXP3 Promoter,Represses
R20404 T27592 T27593 themeOf GATA3,expression
R20405 T27598 T27600 themeOf GATA3 protein,amounts
R20406 T27602 T27604 themeOf GATA3,binding
R20407 T27602 T27605 themeOf GATA3,binding
R20408 T27603 T27605 themeOf FOXP3 promoter,binding
R9519 T12623 T12625 causeOf GATA3,Represses
R9520 T12624 T12625 themeOf FOXP3 Promoter,Represses
R9521 T12626 T12630 causeOf GATA3,repression
R9522 T12627 T12630 themeOf human FOXP3,repression
R9523 T12631 T12632 themeOf FOXP3,regulation
R9524 T12632 T12633 themeOf regulation,role
R9525 T12637 T12640 themeOf GATA3,expressing
R9526 T12639 T12641 themeOf GATA3,express
R9527 T12642 T12645 themeOf GATA3,Overexpression
R9528 T12643 T12646 themeOf luciferase,activity
R9529 T12644 T12647 themeOf FOXP3 promoter,activity
R9530 T12645 T12648 causeOf Overexpression,diminished
R9531 T12645 T12649 causeOf Overexpression,diminished
R9532 T12646 T12648 themeOf activity,diminished
R9533 T12647 T12649 themeOf activity,diminished
R9534 T12652 T12659 themeOf luciferase,increased
R9535 T12660 T12662 themeOf GATA3,binds
R9536 T12660 T12663 themeOf GATA3,binds
R9537 T12661 T12663 themeOf FOXP3 promoter,binds
R9538 T12665 T12668 themeOf GATA3 site,containing
R9539 T12669 T12670 themeOf GATA3,binding
R9540 T12671 T12672 themeOf GATA3,expressing
R9541 T12673 T12675 themeOf GATA3,overexpressing
R9542 T12674 T12676 themeOf GATA3,binding
R544 T644 T646 themeOf Expression,Inhibit
R545 T649 T650 themeOf FOXP3,expression
R546 T650 T651 themeOf expression,regulating
R547 T652 T655 causeOf interleukin 4,inhibits
R548 T653 T656 causeOf IL-4,inhibits
R549 T654 T655 themeOf FOXP3,inhibits
R550 T654 T656 themeOf FOXP3,inhibits
R551 T657 T659 themeOf GATA-3,overexpressing
R552 T658 T660 themeOf FOXP3,induction
R553 T660 T661 themeOf induction,deficient
R554 T662 T664 themeOf GATA3,binding

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Negation Speculation
T102 708-721 Protein denotes interleukin 4
T103 723-727 Protein denotes IL-4
T104 776-781 Protein denotes FOXP3
T105 890-896 Protein denotes GATA-3
T106 944-978 Protein denotes transforming growth factor (TGF)-β
T947 5106-5111 Protein denotes FOXP3
T948 5227-5233 Positive_regulation denotes induce
T949 5234-5239 Protein denotes FOXP3
T950 5240-5250 Gene_expression denotes expression
T951 5356-5361 Protein denotes TGF-β
T952 5480-5487 Negative_regulation denotes lacking
T953 5492-5500 Protein denotes TGF-βRII
T954 5649-5654 Protein denotes TGF-β
T955 5833-5838 Protein denotes GATA3
T956 5843-5848 Protein denotes FOXP3
T957 5901-5906 Protein denotes T-bet
T958 5911-5916 Protein denotes GATA3
T959 5977-5982 Protein denotes GATA3
T960 5983-5991 Negative_regulation denotes inhibits
T961 5992-5997 Protein denotes FOXP3
T962 5998-6007 Positive_regulation denotes induction
T971 6144-6149 Protein denotes FOXP3
T972 6150-6158 Entity denotes promoter
T973 6171-6179 Negative_regulation denotes prevents
T974 6184-6193 Positive_regulation denotes induction
T2899 6290-6299 Positive_regulation denotes Induction
T2900 7185-7193 Positive_regulation denotes increase true
T2901 7200-7210 Gene_expression denotes expression
T2902 7270-7281 Positive_regulation denotes up-regulate
T2903 7980-7987 Gene_expression denotes produce
T2904 8059-8066 Negative_regulation denotes prevent true
T2905 8073-8082 Positive_regulation denotes induction
T2906 8097-8111 Negative_regulation denotes neutralization
T2907 8158-8164 Positive_regulation denotes rescue true
T2908 8171-8181 Gene_expression denotes expression
T2909 8300-8307 Gene_expression denotes express
T2910 6343-6353 Gene_expression denotes expression
T2911 8331-8340 Negative_regulation denotes inability
T2912 8357-8364 Gene_expression denotes express
T2913 8443-8447 Negative_regulation denotes lack
T2914 8454-8464 Gene_expression denotes expression
T2915 8495-8504 Gene_expression denotes expressed
T2916 8586-8593 Gene_expression denotes express
T2917 8609-8612 Positive_regulation denotes low true
T2918 8626-8636 Gene_expression denotes expression
T2919 8681-8689 Positive_regulation denotes increase true
T2920 8700-8710 Gene_expression denotes expression
T2921 6361-6368 Positive_regulation denotes induced
T2922 8748-8755 Positive_regulation denotes induced
T2923 8762-8772 Gene_expression denotes expression
T2924 8882-8891 Gene_expression denotes secretion
T2925 8904-8913 Gene_expression denotes producing
T2926 8943-8950 Negative_regulation denotes lacking
T2927 8957-8967 Gene_expression denotes expression
T2928 9014-9023 Positive_regulation denotes induction
T2929 9024-9030 Negative_regulation denotes failed
T2930 9134-9144 Gene_expression denotes expression true
T2931 9134-9144 Gene_expression denotes expression true
T2932 6524-6531 Gene_expression denotes express
T2933 9261-9271 Gene_expression denotes expressing
T2934 9337-9347 Gene_expression denotes co-express true
T2935 9422-9431 Gene_expression denotes expressed
T2936 9445-9452 Negative_regulation denotes lacking
T2937 9812-9821 Gene_expression denotes expressed
T2938 9871-9880 Gene_expression denotes secretion
T2939 9947-9957 Gene_expression denotes expressing
T2940 10069-10078 Gene_expression denotes expressed true
T2941 10104-10113 Positive_regulation denotes inducible true
T2942 6575-6584 Gene_expression denotes expressed true
T2943 6612-6622 Gene_expression denotes expression
T2944 6626-6636 Regulation denotes restricted true
T2945 6666-6681 Transcription denotes mRNA expression true
T2946 7000-7006 Positive_regulation denotes induce
T2947 6284-6289 Protein denotes FOXP3
T2948 6774-6780 Protein denotes CD45RO
T2949 6923-6926 Protein denotes CD4
T2950 6927-6931 Protein denotes CD25
T2951 6934-6940 Protein denotes CD45RA
T2952 6943-6949 Protein denotes CD45RO
T2953 6956-6959 Protein denotes CD4
T2954 6960-6966 Protein denotes CD45RO
T2955 6967-6971 Protein denotes CD25
T2956 7007-7012 Protein denotes FOXP3
T2957 7068-7073 Protein denotes TGF-β
T2958 6337-6342 Protein denotes FOXP3
T2959 7194-7199 Protein denotes FOXP3
T2960 7282-7287 Protein denotes FOXP3
T2961 7988-7992 Protein denotes IL-4
T2962 8034-8039 Protein denotes TGF-β
T2963 8067-8072 Protein denotes FOXP3
T2964 8115-8119 Protein denotes IL-4
T2965 8136-8140 Protein denotes IL-4
T2966 8165-8170 Protein denotes FOXP3
T2967 8308-8313 Protein denotes FOXP3
T2968 8365-8370 Protein denotes FOXP3
T2969 6485-6488 Protein denotes CD4
T2970 8448-8453 Protein denotes FOXP3
T2971 8505-8510 Protein denotes FOXP3
T2972 8594-8599 Protein denotes FOXP3
T2973 8613-8617 Protein denotes CD25
T2974 8659-8664 Protein denotes TGF-β
T2975 8694-8699 Protein denotes FOXP3
T2976 8756-8761 Protein denotes FOXP3
T2977 8825-8830 Protein denotes CRTH2
T2978 8877-8881 Protein denotes IL-4
T2979 8899-8903 Protein denotes IL-4
T2980 6532-6537 Protein denotes FOXP3
T2981 8951-8956 Protein denotes FOXP3
T2982 9008-9013 Protein denotes FOXP3
T2983 9100-9104 Protein denotes IL-4
T2984 9148-9152 Protein denotes IL-4
T2985 9157-9162 Protein denotes FOXP3
T2986 9183-9186 Protein denotes CD4
T2987 9256-9260 Protein denotes IL-4
T2988 9303-9309 Protein denotes CD45RO
T2989 9310-9314 Protein denotes CD25
T2990 6562-6567 Protein denotes FOXP3
T2991 9348-9353 Protein denotes FOXP3
T2992 9392-9398 Protein denotes CD45RO
T2993 9399-9403 Protein denotes CD25
T2994 9432-9437 Protein denotes FOXP3
T2995 9453-9457 Protein denotes IL-4
T2996 9528-9532 Protein denotes IL-4
T2997 9567-9572 Protein denotes FOXP3
T2998 9609-9613 Protein denotes IL-4
T2999 9642-9647 Protein denotes FOXP3
T3000 9673-9679 Protein denotes CD45RO
T3001 6660-6665 Protein denotes FOXP3
T3002 9680-9684 Protein denotes CD25
T3003 9687-9693 Protein denotes CD45RO
T3004 9694-9698 Protein denotes CD25
T3005 9705-9711 Protein denotes CD45RO
T3006 9712-9716 Protein denotes CD25
T3007 9786-9791 Protein denotes CRTH2
T3008 9822-9827 Protein denotes FOXP3
T3009 9866-9870 Protein denotes IL-4
T3010 9941-9946 Protein denotes FOXP3
T3011 10055-10060 Protein denotes FOXP3
T3012 6731-6735 Protein denotes CD25
T3013 6745-6748 Protein denotes CD4
T3014 6757-6763 Protein denotes CD45RA
T3015 8993-8998 Protein denotes TGF-β
T5187 12413-12416 Positive_regulation denotes low true
T5188 12427-12432 Protein denotes GATA3
T5189 12703-12706 Protein denotes CD3
T6399 13972-13979 Negative_regulation denotes inhibit true
T6400 14518-14525 Positive_regulation denotes induced
T6401 14518-14525 Positive_regulation denotes induced
T6402 14616-14626 Negative_regulation denotes prevention
T6403 14636-14646 Gene_expression denotes expression
T6404 14655-14661 Positive_regulation denotes caused true
T6405 14718-14733 Phosphorylation denotes phosphorylation
T6406 14718-14733 Phosphorylation denotes phosphorylation
T6407 14760-14768 Regulation denotes affected true
T6408 14760-14768 Regulation denotes affected true
T6409 14760-14768 Regulation denotes affected true
T6410 13986-13995 Positive_regulation denotes induction
T6411 14760-14768 Regulation denotes affected true
T6412 14915-14933 Positive_regulation denotes Increasing amounts
T6413 14942-14950 Positive_regulation denotes increase
T6414 14986-14995 Negative_regulation denotes decreased
T6415 15258-15265 Gene_expression denotes express
T6416 16204-16214 Gene_expression denotes expression
T6417 16219-16239 Negative_regulation denotes suppressive capacity true
T6418 16288-16295 Negative_regulation denotes inhibit true
T6419 16302-16312 Gene_expression denotes expression
T6420 16364-16371 Regulation denotes altered true
T6421 14193-14202 Negative_regulation denotes repressed
T6422 16429-16437 Negative_regulation denotes decrease true
T6423 16444-16454 Gene_expression denotes expression
T6424 14222-14231 Positive_regulation denotes induction
T6425 14241-14251 Gene_expression denotes expression
T6426 14338-14345 Negative_regulation denotes induced true
T6427 14358-14365 Negative_regulation denotes absence
T6428 14469-14475 Positive_regulation denotes induce
T6429 14482-14492 Gene_expression denotes expression
T6430 13785-13789 Protein denotes IL-4
T6431 14109-14113 Protein denotes CD28
T6432 14133-14138 Protein denotes TGF-β
T6433 14146-14150 Protein denotes IL-4
T6434 14176-14180 Protein denotes IL-4
T6435 14235-14240 Protein denotes FOXP3
T6436 14327-14332 Protein denotes GATA3
T6437 14369-14373 Protein denotes IL-4
T6438 14441-14445 Protein denotes IL-4
T6439 14476-14481 Protein denotes FOXP3
T6440 13799-13804 Protein denotes TGF-β
T6441 14503-14508 Protein denotes GATA3
T6442 14545-14550 Protein denotes TGF-β
T6443 14559-14563 Protein denotes IL-4
T6444 14602-14606 Protein denotes IL-4
T6445 14630-14635 Protein denotes FOXP3
T6446 14737-14742 Protein denotes SMAD2
T6447 14746-14751 Protein denotes STAT6
T6448 14788-14792 Protein denotes IL-4
T6449 14800-14805 Protein denotes TGF-β
T6450 14831-14835 Protein denotes IL-4
T6451 13831-13835 Protein denotes IL-4
T6452 14847-14852 Protein denotes TGF-β
T6453 14937-14941 Protein denotes IL-4
T6454 14965-14970 Protein denotes GATA3
T6455 14980-14985 Protein denotes FOXP3
T6456 15079-15083 Protein denotes IL-4
T6457 15274-15278 Protein denotes CD25
T6458 15319-15323 Protein denotes IL-4
T6459 15325-15331 Protein denotes rmIL-4
T6460 15343-15347 Protein denotes IL-4
T6461 15500-15503 Protein denotes CD4
T6462 13956-13960 Protein denotes IL-4
T6463 15504-15508 Protein denotes CD25
T6464 15514-15519 Protein denotes FOXP3
T6465 15646-15652 Protein denotes rmIL-4
T6466 15663-15667 Protein denotes IL-4
T6467 15703-15706 Protein denotes CD4
T6468 15707-15711 Protein denotes CD25
T6469 15736-15741 Protein denotes Foxp3
T6470 15863-15866 Protein denotes CD4
T6471 15867-15871 Protein denotes CD25
T6472 15903-15906 Protein denotes CD4
T6473 13980-13985 Protein denotes TGF-β
T6474 15907-15911 Protein denotes CD25
T6475 15937-15941 Protein denotes IL-4
T6476 16067-16071 Protein denotes IL-4
T6477 16149-16153 Protein denotes CD25
T6478 16180-16184 Protein denotes IL-4
T6479 16198-16203 Protein denotes FOXP3
T6480 16273-16277 Protein denotes IL-4
T6481 16296-16301 Protein denotes FOXP3
T6482 16438-16443 Protein denotes FOXP3
T6483 16460-16464 Protein denotes IL-4
T6484 13999-14004 Protein denotes FOXP3
T6485 14048-14051 Protein denotes CD4
T6486 14052-14058 Protein denotes CD45RA
T6487 14105-14108 Protein denotes CD3
T8280 17604-17611 Negative_regulation denotes reduced
T8281 18268-18278 Gene_expression denotes expression
T8282 18287-18293 Positive_regulation denotes caused true
T8283 18517-18531 Negative_regulation denotes down-regulated
T8284 18605-18615 Positive_regulation denotes pronounced
T8285 19424-19433 Gene_expression denotes producing
T8286 19454-19464 Gene_expression denotes expressing
T8287 19613-19623 Gene_expression denotes expression true
T8288 19613-19623 Gene_expression denotes expression true
T8289 19691-19703 Positive_regulation denotes demonstrated
T8290 19794-19804 Gene_expression denotes co-express
T8291 17623-17633 Gene_expression denotes expression
T8292 19691-19703 Positive_regulation denotes demonstrated
T8293 19691-19703 Positive_regulation denotes demonstrated
T8294 19794-19804 Gene_expression denotes co-express
T8295 19691-19703 Positive_regulation denotes demonstrated
T8296 19999-20009 Negative_regulation denotes repressing true
T8339 19950-19955 Protein denotes GATA3
T8340 17618-17622 Protein denotes IL-4
T8341 20010-20015 Protein denotes FOXP3
T8342 20043-20047 Protein denotes IL-4
T8343 20081-20086 Protein denotes FOXP3
T8344 20124-20129 Protein denotes TGF-β
T8345 20214-20219 Protein denotes GATA3
T8346 17639-17643 Protein denotes CD25
T8347 17668-17672 Protein denotes IL-4
T8348 17697-17702 Protein denotes TGF-β
T8349 17725-17730 Protein denotes FOXP3
T8350 17762-17767 Protein denotes FOXP3
T8351 17598-17603 Protein denotes TGF-β
T9761 20330-20340 Gene_expression denotes expression
T9762 20344-20348 Positive_regulation denotes high
T9763 20389-20393 Regulation denotes role true
T9764 20398-20403 Protein denotes GATA3
T9765 20407-20417 Negative_regulation denotes repressing
T9766 20418-20423 Protein denotes FOXP3
T9767 20433-20438 Protein denotes GATA3
T9768 20488-20493 Protein denotes GATA3
T9769 20572-20582 Negative_regulation denotes inhibiting
T9770 20583-20588 Protein denotes STAT4
T9771 20589-20599 Gene_expression denotes expression
T9772 20622-20627 Protein denotes GATA3
T9773 20692-20697 Protein denotes GATA3
T9774 20711-20718 Negative_regulation denotes inhibit true
T9775 20719-20724 Protein denotes FOXP3
T9776 20725-20734 Positive_regulation denotes induction
T9777 20750-20755 Protein denotes GATA3
T9778 20771-20776 Protein denotes GATA3
T9779 20777-20784 Negative_regulation denotes lacking
T9780 20789-20807 Entity denotes DNA-binding domain
T9781 20825-20828 Protein denotes CD4
T9782 20829-20835 Protein denotes CD45RA
T9783 20853-20893 Protein denotes TAT-fused, recombinantly expressed GATA3
T9784 20958-20961 Protein denotes CD3
T9785 20962-20966 Protein denotes CD28
T9786 20997-21002 Protein denotes TGF-β
T9787 21004-21013 Protein denotes TAT-GATA3
T9788 21096-21099 Protein denotes CD4
T9789 21135-21144 Protein denotes TAT-GATA3
T9790 21145-21152 Negative_regulation denotes reduced
T9791 21153-21158 Protein denotes FOXP3
T9792 21159-21169 Gene_expression denotes expression
T9793 21208-21226 Entity denotes DNA-binding domain
T9794 21227-21236 Negative_regulation denotes truncated
T9795 21255-21260 Protein denotes GATA3
T9796 21270-21276 Regulation denotes affect true
T9797 21277-21282 Protein denotes FOXP3
T9798 21283-21293 Gene_expression denotes expression
T9799 21386-21403 Negative_regulation denotes inhibitory effect true
T9800 21407-21412 Protein denotes GATA3
T9801 21416-21421 Protein denotes FOXP3
T9802 21465-21479 Positive_regulation denotes overexpressing
T9803 21480-21485 Protein denotes GATA3
T9804 21511-21514 Protein denotes CD2
T9805 21545-21548 Protein denotes CD2
T9806 21549-21554 Protein denotes GATA3
T9807 21587-21590 Protein denotes CD4
T9808 21628-21631 Protein denotes CD2
T9809 21632-21637 Protein denotes GATA3
T9810 21793-21799 Regulation denotes effect true
T9811 21803-21808 Protein denotes GATA3
T9812 21819-21822 Protein denotes CD4
T9813 21823-21828 Protein denotes CD62L
T9814 21829-21833 Protein denotes CD25
T9815 21905-21910 Protein denotes TGF-β
T9816 21916-21921 Protein denotes Foxp3
T9817 21922-21932 Gene_expression denotes expression true
T9818 21967-21970 Protein denotes CD4
T9819 21971-21975 Protein denotes CD25
T9820 21988-21993 Protein denotes Foxp3
T9821 22049-22054 Protein denotes TGF-β
T9822 22068-22080 Positive_regulation denotes up-regulated
T9823 22081-22086 Protein denotes Foxp3
T9824 22155-22158 Protein denotes CD2
T9825 22159-22164 Protein denotes GATA3
T9826 22198-22205 Negative_regulation denotes reduced
T9827 22206-22211 Protein denotes Foxp3
T9828 22212-22222 Gene_expression denotes expression
T9829 22228-22237 Positive_regulation denotes activated
T9830 22243-22248 Protein denotes TGF-β
T9831 22279-22287 Gene_expression denotes produced
T9832 22307-22312 Protein denotes TGF-β
T9833 22327-22332 Protein denotes Smad7
T9834 22345-22354 Gene_expression denotes expressed
T9835 22423-22428 Protein denotes TGF-β
T9836 23382-23386 Protein denotes IL-4
T9837 23387-23394 Positive_regulation denotes induced
T9838 23395-23400 Protein denotes GATA3
T11552 25462-25469 Binding denotes binding
T11553 25535-25540 Protein denotes GATA3
T11554 25541-25546 Binding denotes binds
T11555 25563-25568 Protein denotes FOXP3
T11556 25569-25577 Entity denotes promoter
T11557 25690-25695 Protein denotes GATA3
T11558 25721-25726 Protein denotes GATA3
T11559 25727-25732 Binding denotes binds
T11560 25740-25745 Protein denotes FOXP3
T11561 25746-25761 Entity denotes promoter region
T11562 25859-25864 Protein denotes GATA3
T11563 25865-25872 Binding denotes binding
T11564 25880-25885 Protein denotes FOXP3
T11565 25898-25908 Negative_regulation denotes repressing
T11566 25909-25914 Protein denotes FOXP3
T11567 25915-25925 Gene_expression denotes expression
T13498 27662-27671 Positive_regulation denotes induction
T13499 28712-28722 Gene_expression denotes expression
T13500 28712-28722 Gene_expression denotes expression
T13501 28866-28876 Gene_expression denotes expression
T13502 28866-28876 Gene_expression denotes expression
T13503 28896-28899 Negative_regulation denotes low true
T13504 28896-28899 Negative_regulation denotes low true
T13505 28959-28966 Negative_regulation denotes absence
T13506 28959-28966 Negative_regulation denotes absence
T13507 28959-28966 Negative_regulation denotes absence
T13508 29057-29067 Gene_expression denotes expression
T13509 27715-27724 Negative_regulation denotes inhibited
T13510 29113-29123 Gene_expression denotes expressing
T13511 29156-29168 Positive_regulation denotes up-regulated
T13512 29156-29168 Positive_regulation denotes up-regulated
T13513 29249-29258 Positive_regulation denotes promoting
T13514 29347-29357 Negative_regulation denotes inhibitors
T13515 29367-29377 Gene_expression denotes expression
T13516 29460-29468 Negative_regulation denotes inhibits
T13517 29475-29485 Gene_expression denotes expression
T13518 29586-29593 Negative_regulation denotes inhibit
T13519 29615-29624 Positive_regulation denotes induction
T13520 27977-27988 Positive_regulation denotes up-regulate
T13521 29586-29593 Negative_regulation denotes inhibit true
T13522 30259-30266 Gene_expression denotes express
T13523 30327-30334 Negative_regulation denotes inhibit true
T13524 30341-30351 Gene_expression denotes expression
T13525 30406-30416 Positive_regulation denotes contribute true
T13526 30433-30437 Negative_regulation denotes drop
T13527 30447-30457 Gene_expression denotes expression
T13528 30565-30574 Gene_expression denotes producing
T13529 31130-31138 Positive_regulation denotes enhanced
T13530 31145-31155 Gene_expression denotes expression
T13531 28106-28115 Positive_regulation denotes induction
T13532 31214-31220 Positive_regulation denotes induce true
T13533 31227-31237 Gene_expression denotes expression
T13534 31300-31308 Regulation denotes regulate true
T13535 31315-31325 Gene_expression denotes expression
T13536 31393-31399 Negative_regulation denotes reduce true
T13537 31419-31425 Positive_regulation denotes induce true
T13538 31473-31483 Negative_regulation denotes inhibition
T13539 31502-31510 Positive_regulation denotes mediated true
T13540 31536-31544 Positive_regulation denotes inducing
T13541 31565-31574 Negative_regulation denotes repressed
T13542 28246-28257 Positive_regulation denotes up-regulate
T13543 31581-31589 Positive_regulation denotes mediated
T13544 31596-31606 Gene_expression denotes expression
T13545 31869-31873 Positive_regulation denotes high
T13546 31880-31890 Gene_expression denotes expressing
T13547 31915-31922 Negative_regulation denotes absence
T13548 31982-31991 Negative_regulation denotes inhibited
T13549 31998-32007 Positive_regulation denotes induction
T13550 32131-32141 Gene_expression denotes expressing
T13551 32223-32237 Positive_regulation denotes overexpression
T13552 32285-32291 Negative_regulation denotes failed
T13553 28546-28556 Gene_expression denotes expression
T13554 32295-32301 Positive_regulation denotes induce
T13555 32308-32318 Gene_expression denotes expression
T13556 32781-32795 Positive_regulation denotes overexpression
T13557 33293-33302 Negative_regulation denotes repressed
T13558 33309-33319 Gene_expression denotes expression
T13559 33332-33339 Binding denotes binding
T13560 33706-33713 Gene_expression denotes express
T13561 33783-33790 Gene_expression denotes express true
T13562 33831-33836 Binding denotes bound
T13563 33936-33941 Binding denotes binds
T13564 28546-28556 Gene_expression denotes expression
T13565 34209-34219 Gene_expression denotes expression
T13566 34220-34223 Negative_regulation denotes low
T13567 34337-34344 Negative_regulation denotes inhibit
T13568 34500-34507 Negative_regulation denotes compete true
T13569 34517-34524 Binding denotes binding
T13570 34500-34507 Negative_regulation denotes compete true
T13571 34517-34524 Binding denotes binding
T13572 34624-34644 Negative_regulation denotes negatively regulated
T13573 34678-34698 Negative_regulation denotes acts as an inhibitor
T13574 34708-34718 Gene_expression denotes expression
T13575 28665-28678 Gene_expression denotes co-expression
T13576 34803-34810 Binding denotes binding
T13577 34815-34825 Negative_regulation denotes repressing
T13578 34926-34936 Negative_regulation denotes repressing
T13579 34943-34953 Gene_expression denotes expression
T13580 28665-28678 Gene_expression denotes co-expression
T13581 27656-27661 Protein denotes FOXP3
T13582 28338-28343 Protein denotes FOXP3
T13583 33347-33352 Protein denotes FOXP3
T13584 33505-33510 Protein denotes FOXP3
T13585 33714-33719 Protein denotes GATA3
T13586 33791-33796 Protein denotes GATA3
T13587 33840-33845 Protein denotes GATA3
T13588 33930-33935 Protein denotes GATA3
T13589 33994-33999 Protein denotes GATA3
T13590 34203-34208 Protein denotes GATA3
T13591 34262-34267 Protein denotes FOXP3
T13592 34328-34333 Protein denotes GATA3
T13593 28481-28486 Protein denotes T-bet
T13594 34345-34350 Protein denotes FOXP3
T13595 34490-34495 Protein denotes GATA3
T13596 34528-34532 Protein denotes AP-1
T13597 34533-34537 Protein denotes NFAT
T13598 34615-34620 Protein denotes FOXP3
T13599 34666-34670 Protein denotes IL-4
T13600 34672-34677 Protein denotes GATA3
T13601 34702-34707 Protein denotes FOXP3
T13602 34830-34835 Protein denotes FOXP3
T13603 34891-34895 Protein denotes Il-4
T13604 28491-28496 Protein denotes GATA3
T13605 34937-34942 Protein denotes FOXP3
T13606 28625-28630 Protein denotes FOXP3
T13607 28783-28788 Protein denotes TGF-β
T13608 28835-28840 Protein denotes FOXP3
T13609 28880-28885 Protein denotes GATA3
T13610 28890-28895 Protein denotes T-bet
T13611 29005-29009 Protein denotes IL-4
T13612 33353-33368 Entity denotes promoter region
T13613 29011-29016 Protein denotes IL-12
T13614 34545-34553 Entity denotes promoter
T13615 34836-34844 Entity denotes promoter
T13616 27728-27733 Protein denotes GATA3
T13617 29021-29026 Protein denotes TGF-β
T13618 29051-29056 Protein denotes FOXP3
T13619 29107-29112 Protein denotes FOXP3
T13620 29135-29140 Protein denotes GATA3
T13621 29145-29150 Protein denotes T-bet
T13622 29243-29248 Protein denotes FOXP3
T13623 29296-29302 Protein denotes CTLA-4
T13624 29361-29366 Protein denotes GATA3
T13625 29397-29402 Protein denotes GATA3
T13626 29469-29474 Protein denotes FOXP3
T13627 27882-27887 Protein denotes FOXP3
T13628 29536-29540 Protein denotes IL-4
T13629 29549-29554 Protein denotes IL-13
T13630 29609-29614 Protein denotes FOXP3
T13631 29725-29729 Protein denotes IL-4
T13632 29800-29804 Protein denotes IL-4
T13633 29814-29818 Protein denotes IL-4
T13634 29902-29906 Protein denotes IL-4
T13635 29907-29911 Protein denotes IL-4
T13636 29991-29995 Protein denotes CD25
T13637 27948-27951 Protein denotes CD4
T13638 30001-30006 Protein denotes Foxp3
T13639 30061-30065 Protein denotes IL-4
T13640 30267-30271 Protein denotes CD25
T13641 30313-30317 Protein denotes IL-4
T13642 30335-30340 Protein denotes Foxp3
T13643 30441-30446 Protein denotes Foxp3
T13644 30459-30463 Protein denotes IL-4
T13645 30559-30564 Protein denotes IL-17
T13646 30648-30652 Protein denotes IL-6
T13647 30675-30680 Protein denotes TGF-β
T13648 27952-27956 Protein denotes CD25
T13649 30891-30895 Protein denotes IL-4
T13650 30900-30904 Protein denotes IL-6
T13651 30906-30910 Protein denotes IL-4
T13652 30965-30970 Protein denotes FOXP3
T13653 30990-30993 Protein denotes CD4
T13654 30994-30998 Protein denotes CD25
T13655 31050-31054 Protein denotes IL-4
T13656 31095-31099 Protein denotes IL-4
T13657 31139-31144 Protein denotes FOXP3
T13658 31221-31226 Protein denotes GATA3
T13659 27989-27994 Protein denotes FOXP3
T13660 31239-31243 Protein denotes IL-4
T13661 31309-31314 Protein denotes FOXP3
T13662 31366-31371 Protein denotes IL-13
T13663 31400-31405 Protein denotes FOXP3
T13664 31426-31431 Protein denotes GATA3
T13665 31458-31462 Protein denotes IL-4
T13666 31487-31492 Protein denotes FOXP3
T13667 31514-31519 Protein denotes GATA3
T13668 31530-31535 Protein denotes GATA3
T13669 31545-31549 Protein denotes IL-4
T13670 28100-28105 Protein denotes FOXP3
T13671 31590-31595 Protein denotes FOXP3
T13672 31616-31620 Protein denotes IL-4
T13673 31632-31637 Protein denotes TGF-β
T13674 31715-31720 Protein denotes GATA3
T13675 31725-31730 Protein denotes FOXP3
T13676 31874-31879 Protein denotes GATA3
T13677 31926-31931 Protein denotes FOXP3
T13678 31957-31962 Protein denotes GATA3
T13679 31992-31997 Protein denotes FOXP3
T13680 28258-28263 Protein denotes FOXP3
T13681 32076-32081 Protein denotes GATA3
T13682 32142-32147 Protein denotes GATA3
T13683 32168-32171 Protein denotes CD2
T13684 32172-32177 Protein denotes GATA3
T13685 32241-32246 Protein denotes GATA3
T13686 32302-32307 Protein denotes FOXP3
T13687 32365-32370 Protein denotes TGF-β
T13688 32396-32399 Protein denotes CD2
T13689 32400-32405 Protein denotes GATA3
T13690 32430-32435 Protein denotes FOXP3
T13691 28282-28287 Protein denotes TGF-β
T13692 32535-32540 Protein denotes GATA3
T13693 32775-32780 Protein denotes GATA3
T13694 32874-32879 Protein denotes GATA3
T13695 32903-32908 Protein denotes GATA3
T13696 32982-33000 Protein denotes Repressor of GATA3
T13697 33002-33005 Protein denotes ROG
T13698 33045-33048 Protein denotes CD8
T13699 33196-33201 Protein denotes GATA3
T13700 33287-33292 Protein denotes GATA3
T13701 33303-33308 Protein denotes FOXP3
T13702 29594-29599 Protein denotes TGF-β
T13703 31575-31580 Protein denotes TGF-β
T18780 37820-37823 Protein denotes CD4
T18781 37834-37837 Protein denotes CD4
T18782 37840-37845 Protein denotes CD62L
T18783 37852-37856 Protein denotes CD25
T18784 38120-38126 Protein denotes TGF-ß1
T18785 38221-38226 Protein denotes FOXP3
T18786 38227-38237 Gene_expression denotes expression true
T20428 40747-40750 Protein denotes TAT
T20429 40751-40756 Protein denotes GATA3
T20430 40771-40774 Protein denotes CD4
T20431 40775-40781 Protein denotes CD45RA
T20432 40890-40895 Protein denotes GATA3
T20433 40985-40988 Protein denotes CD3
T20434 40998-41002 Protein denotes CD28
T20435 41007-41012 Protein denotes TGF-β
T20436 41039-41042 Protein denotes TAT
T20437 41086-41091 Protein denotes FOXP3
T20438 41092-41102 Gene_expression denotes expression true
T21774 43267-43272 Protein denotes FOXP3
T21775 43312-43315 Protein denotes CD4
T21776 43316-43320 Protein denotes CD25
T21777 43651-43656 Protein denotes FOXP3
T21778 43941-43946 Protein denotes GATA3
T21779 43988-43993 Protein denotes T-bet
T21780 44033-44038 Protein denotes GAPDH
T21781 44067-44072 Protein denotes SMAD2
T21782 44095-44100 Protein denotes STAT6
T21783 44117-44122 Protein denotes STAT6
T23452 7587-7592 Protein denotes FOXP3
T23453 7763-7766 Protein denotes CD3
T23454 7767-7771 Protein denotes CD28
T23455 7773-7778 Protein denotes TGF-β
T23456 7788-7792 Protein denotes IL-4
T24110 10826-10836 Gene_expression denotes expression
T24111 10892-10898 Protein denotes CD45RO
T24112 10899-10903 Protein denotes CD25
T24113 10926-10932 Protein denotes CD45RO
T24114 10933-10937 Protein denotes CD25
T24115 10962-10968 Protein denotes CD45RO
T24116 10969-10973 Protein denotes CD25
T24117 11243-11248 Protein denotes CRTH2
T24118 11261-11265 Protein denotes IL-4
T24119 11266-11275 Gene_expression denotes secreting
T24120 11303-11309 Protein denotes CD45RO
T24121 11316-11323 Gene_expression denotes stained
T24122 11316-11323 Gene_expression denotes stained
T24123 11328-11333 Protein denotes FOXP3
T24124 11338-11342 Protein denotes IL-4
T25140 17327-17332 Protein denotes GAPDH
T25141 17379-17384 Protein denotes GATA3
T25142 17389-17394 Protein denotes FOXP3
T25143 17395-17403 Gene_expression denotes staining
T25144 17395-17403 Gene_expression denotes staining
T25145 17432-17435 Protein denotes CD4
T25146 17436-17442 Protein denotes CD45RA
T25147 17455-17459 Protein denotes IL-4
T25862 19117-19125 Gene_expression denotes staining true
T25863 19117-19125 Gene_expression denotes staining true
T25864 18665-18669 Protein denotes IL-4
T25865 19111-19116 Protein denotes FOXP3
T25866 19168-19171 Protein denotes CD4
T25867 19172-19178 Protein denotes CD45RA
T25868 19196-19199 Protein denotes CD3
T25869 19200-19202 Protein denotes 28
T25870 19204-19208 Protein denotes IL-4
T25871 19213-19218 Protein denotes TGF-β
T25872 18724-18727 Protein denotes CD4
T25873 18728-18734 Protein denotes CD45RA
T25874 18779-18782 Protein denotes CD3
T25875 18783-18787 Protein denotes CD28
T25876 18789-18794 Protein denotes TGF-β
T25877 18800-18804 Protein denotes IL-4
T25878 19101-19106 Protein denotes GATA3
T25879 18679-18684 Protein denotes TGF-β
T11550 25429-25434 Protein denotes GATA3
T11500 23457-23462 Protein denotes GATA3
T11501 23463-23472 Negative_regulation denotes Represses
T11502 23477-23482 Protein denotes FOXP3
T11503 23483-23491 Entity denotes Promoter
T11504 23534-23539 Protein denotes GATA3
T11505 23549-23559 Negative_regulation denotes repression true
T11506 23569-23574 Protein denotes FOXP3
T11507 23586-23591 Protein denotes FOXP3
T11508 23648-23653 Protein denotes GATA3
T11509 23674-23691 Entity denotes GATA-binding site
T11510 23856-23878 Regulation denotes play an important role true
T11511 23882-23887 Protein denotes FOXP3
T11512 23888-23898 Regulation denotes regulation
T11513 23958-23963 Protein denotes FOXP3
T11514 24018-24021 Protein denotes CD4
T11515 24126-24133 Gene_expression denotes express
T11516 24134-24139 Protein denotes GATA3
T11517 24166-24171 Protein denotes FOXP3
T11518 24172-24180 Entity denotes promoter
T11519 24226-24231 Protein denotes GATA3
T11520 24232-24242 Gene_expression denotes expressing
T11521 24331-24337 Positive_regulation denotes active
T11522 24345-24348 Protein denotes CD4
T11523 24362-24369 Gene_expression denotes express true
T11524 24388-24393 Protein denotes GATA3
T11525 24407-24421 Positive_regulation denotes Overexpression
T11526 24425-24430 Protein denotes GATA3
T11527 24486-24491 Protein denotes FOXP3
T11528 24635-24643 Negative_regulation denotes deleting
T11529 24648-24666 Entity denotes GATA3-binding site
T11530 24732-24735 Protein denotes CD4
T11531 24736-24742 Protein denotes CD45RO
T11532 24798-24803 Protein denotes GATA3
T11533 24806-24809 Protein denotes CD4
T11534 24810-24816 Protein denotes CD45RA
T11535 24839-24848 Negative_regulation denotes repressor
T11536 24861-24866 Protein denotes GATA3
T11537 24874-24879 Protein denotes FOXP3
T11538 24880-24888 Entity denotes promoter
T11539 24914-24919 Protein denotes GATA3
T11540 24920-24925 Binding denotes binds
T11541 24942-24947 Protein denotes FOXP3
T11542 24948-24956 Entity denotes promoter
T11543 25037-25042 Protein denotes GATA3
T11544 25168-25173 Protein denotes FOXP3
T11545 25268-25273 Protein denotes GATA3
T11546 25274-25281 Binding denotes binding true
T11547 25323-25328 Protein denotes GATA3
T11548 25329-25339 Gene_expression denotes expressing
T11549 25414-25428 Gene_expression denotes overexpressing
T11551 25456-25461 Protein denotes GATA3
T23801 10157-10164 Gene_expression denotes Express true
T23802 10165-10170 Protein denotes FOXP3
T23803 10206-10211 Protein denotes FOXP3
T23804 10212-10222 Gene_expression denotes expression true
T23805 10342-10347 Protein denotes TGF-β
T23806 10349-10354 Protein denotes FOXP3
T23807 10355-10365 Gene_expression denotes expression true
T22563 45111-45116 Protein denotes GATA3
T22564 45490-45495 Protein denotes FOXP3
T22965 45716-45719 Protein denotes CD4
T22966 46599-46604 Protein denotes GATA3
T23446 7404-7410 Positive_regulation denotes Induce true
T23447 7411-7416 Protein denotes FOXP3
T23448 7417-7427 Gene_expression denotes Expression
T23449 7483-7486 Protein denotes CD3
T23450 7487-7491 Protein denotes CD28
T23451 7508-7513 Protein denotes TGF-β
T5159 11403-11408 Protein denotes FOXP3
T5160 11413-11418 Protein denotes GATA3
T5161 11510-11516 Positive_regulation denotes induce
T5162 11517-11522 Protein denotes FOXP3
T5163 11523-11533 Gene_expression denotes expression
T5164 11651-11661 Gene_expression denotes expression true
T5165 11651-11661 Gene_expression denotes expression true
T5166 11665-11670 Protein denotes FOXP3
T5167 11675-11680 Protein denotes GATA3
T5168 11717-11720 Protein denotes CD4
T5169 11753-11757 Protein denotes IL-4
T5170 11764-11769 Protein denotes IFN-γ
T5171 11780-11785 Protein denotes IL-12
T5172 11864-11869 Protein denotes FOXP3
T5173 11874-11879 Protein denotes GATA3
T5174 11897-11907 Gene_expression denotes expression
T5175 11897-11907 Gene_expression denotes expression
T5176 12039-12044 Protein denotes FOXP3
T5177 12045-12060 Transcription denotes mRNA expression
T5178 12061-12070 Positive_regulation denotes increased
T5179 12127-12132 Protein denotes GATA3
T5180 12137-12142 Protein denotes FOXP3
T5181 12174-12184 Gene_expression denotes expression
T5182 12174-12184 Gene_expression denotes expression
T5183 12357-12367 Gene_expression denotes expressing
T5184 12368-12372 Positive_regulation denotes more
T5185 12373-12378 Protein denotes FOXP3
T5186 12403-12412 Gene_expression denotes expressed
T5190 12736-12741 Protein denotes CD103
T5191 12743-12749 Protein denotes CTLA-4
T5192 12751-12755 Protein denotes GITR
T5193 12761-12765 Protein denotes PD-1
T5194 12774-12784 Gene_expression denotes expression
T5195 12774-12784 Gene_expression denotes expression
T5196 12774-12784 Gene_expression denotes expression
T5197 12774-12784 Gene_expression denotes expression
T5198 12894-12899 Protein denotes GATA3
T5199 12904-12909 Protein denotes FOXP3
T5200 12910-12920 Gene_expression denotes expression
T5201 12910-12920 Gene_expression denotes expression
T5202 12972-12980 Negative_regulation denotes resolved
T5203 12972-12980 Negative_regulation denotes resolved
T5204 13079-13083 Negative_regulation denotes lost
T5205 13102-13109 Gene_expression denotes express
T5206 13110-13115 Protein denotes FOXP3
T920 3496-3509 Protein denotes interleukin 4
T921 3511-3515 Protein denotes IL-4
T922 3517-3524 Positive_regulation denotes induced
T923 3517-3524 Positive_regulation denotes induced
T924 3525-3530 Protein denotes STAT6
T925 3535-3540 Protein denotes GATA3
T926 3620-3625 Protein denotes GATA3
T927 3700-3709 Positive_regulation denotes induction
T928 3700-3709 Positive_regulation denotes induction
T929 3700-3709 Positive_regulation denotes induction
T930 3713-3717 Protein denotes IL-4
T931 3719-3723 Protein denotes IL-5
T932 3728-3733 Protein denotes IL-13
T933 3848-3853 Protein denotes T-bet
T934 3863-3872 Regulation denotes modulates
T935 3873-3878 Protein denotes GATA3
T936 4077-4082 Protein denotes GATA3
T937 4177-4181 Protein denotes IL-4
T938 4251-4293 Negative_regulation denotes Overexpression of a dominant negative form
T939 4297-4302 Protein denotes GATA3
T940 4344-4349 Protein denotes GATA3
T941 4350-4358 Negative_regulation denotes blockade
T942 4756-4763 Gene_expression denotes express
T943 4798-4803 Protein denotes FOXP3
T944 4823-4830 Gene_expression denotes express
T945 4831-4835 Protein denotes CD25
T946 5098-5105 Gene_expression denotes express
T963 6017-6021 Protein denotes IL-4
T964 6022-6028 Negative_regulation denotes limits
T965 6029-6034 Protein denotes FOXP3
T966 6035-6045 Gene_expression denotes expression
T967 6051-6056 Protein denotes GATA3
T968 6057-6069 Positive_regulation denotes mediated way
T969 6116-6121 Protein denotes GATA3
T970 6131-6136 Binding denotes binds
T26903 25937-25942 Protein denotes GATA3
T26904 25943-25952 Negative_regulation denotes Represses
T26905 25963-25968 Protein denotes FOXP3
T26906 25969-25977 Entity denotes Promoter
T26907 26019-26022 Protein denotes CD4
T26908 26116-26121 Protein denotes FOXP3
T26909 26151-26161 Protein denotes luciferase
T26910 26249-26259 Protein denotes luciferase
T26911 26341-26344 Protein denotes CD4
T26912 26379-26384 Protein denotes FOXP3
T26913 26429-26434 Protein denotes GATA3
T26914 26435-26445 Gene_expression denotes expression
T26915 26578-26581 Protein denotes CD4
T26916 26627-26632 Protein denotes GATA3
T26917 26645-26650 Protein denotes FOXP3
T26918 26789-26799 Protein denotes luciferase
T26919 26939-26944 Protein denotes GATA3
T26920 27154-27161 Gene_expression denotes amounts true
T26921 27165-27170 Protein denotes GATA3
T26922 27241-27246 Protein denotes GATA3
T26923 27407-27412 Protein denotes GATA3
T26924 27413-27420 Binding denotes binding true
T26925 27428-27433 Protein denotes FOXP3
T26926 27434-27442 Entity denotes promoter
T19755 39467-39472 Protein denotes FOXP3
T19756 39482-39492 Protein denotes luciferase
T19757 39550-39553 Protein denotes CD4
T19758 39779-39789 Protein denotes luciferase
T19759 39824-39834 Protein denotes luciferase
T19760 39960-39970 Protein denotes luciferase
T9753 20242-20247 Protein denotes GATA3
T25118 16498-16504 Regulation denotes Effect
T25119 16508-16512 Protein denotes IL-4
T25120 16516-16521 Protein denotes FOXP3
T25121 16522-16531 Positive_regulation denotes Induction
T25122 16599-16604 Protein denotes TGF-β
T25123 16636-16640 Protein denotes IL-4
T25124 16869-16872 Protein denotes CD4
T25125 16873-16879 Protein denotes CD45RA
T25126 16949-16954 Protein denotes TGF-β
T25127 16973-16983 Positive_regulation denotes increasing
T25128 17001-17005 Protein denotes IL-4
T25129 17081-17084 Protein denotes CD4
T25130 17085-17091 Protein denotes CD45RA
T25131 17146-17149 Protein denotes CD3
T25132 17150-17154 Protein denotes CD28
T25133 17156-17161 Protein denotes TGF-β
T25134 17177-17181 Protein denotes IL-4
T25135 17279-17293 Phosphorylation denotes phosphorylated true
T25136 17279-17293 Phosphorylation denotes phosphorylated true
T25137 17294-17299 Protein denotes SMAD2
T25138 17304-17309 Protein denotes STAT6
T25139 17317-17322 Protein denotes STAT6
T9754 20253-20271 Negative_regulation denotes Negative Regulator
T9755 20275-20280 Protein denotes FOXP3
T9756 20281-20291 Gene_expression denotes Expression
T9757 20292-20297 Protein denotes FOXP3
T9758 20298-20308 Gene_expression denotes expression
T9759 20309-20318 Negative_regulation denotes decreased
T9760 20324-20329 Protein denotes GATA3
T24681 13213-13218 Protein denotes FOXP3
T24682 13219-13228 Positive_regulation denotes Induction
T24683 13274-13277 Protein denotes CD4
T24684 13278-13284 Protein denotes CD45RA
T24685 13331-13334 Protein denotes CD3
T24686 13335-13339 Protein denotes CD28
T24687 13359-13364 Protein denotes TGF-β
T24688 13378-13382 Protein denotes IL-4
T24689 13491-13496 Protein denotes FOXP3
T24690 13501-13506 Protein denotes GATA3
T24691 13507-13517 Gene_expression denotes expression true
T24692 13507-13517 Gene_expression denotes expression true
T24693 13596-13601 Protein denotes GATA3
T24694 13606-13611 Protein denotes FOXP3
T24695 13612-13620 Gene_expression denotes staining true
T24696 13612-13620 Gene_expression denotes staining true
T24697 13648-13651 Protein denotes CD4
T24698 13652-13658 Protein denotes CD45RA
T24098 10632-10636 Protein denotes IL-4
T24099 10637-10646 Gene_expression denotes Producing
T24100 10653-10657 Negative_regulation denotes Lack
T24101 10658-10663 Protein denotes FOXP3
T24102 10699-10704 Protein denotes FOXP3
T24103 10709-10713 Protein denotes IL-4
T24104 10714-10724 Gene_expression denotes expression true
T24105 10714-10724 Gene_expression denotes expression true
T24106 10762-10765 Protein denotes CD4
T24107 10802-10808 Protein denotes CD45RO
T24108 10813-10817 Protein denotes CD25
T24109 10826-10836 Gene_expression denotes expression
T20709 41316-41320 Protein denotes IL-4
T26314 22450-22455 Protein denotes GATA3
T26315 22488-22493 Protein denotes FOXP3
T26316 22521-22524 Protein denotes CD4
T26317 22525-22529 Protein denotes CDRA
T26318 22586-22595 Protein denotes TAT-GATA3
T26319 22623-22631 Gene_expression denotes presence true
T26320 22635-22640 Protein denotes GATA3
T26321 22680-22683 Protein denotes CD3
T26322 22684-22688 Protein denotes CD28
T26323 22714-22717 Protein denotes GFP
T26324 22775-22780 Protein denotes FOXP3
T26325 22781-22791 Gene_expression denotes expression true
T26326 22902-22905 Protein denotes CD4
T26327 22906-22910 Protein denotes CD25
T26328 22959-22962 Protein denotes CD2
T26329 22963-22968 Protein denotes GATA3
T26330 22999-23004 Protein denotes TGF-β
T26331 23023-23026 Protein denotes CD4
T26332 23045-23050 Protein denotes FOXP3
T26333 23230-23235 Protein denotes SMAD7
T26334 23240-23245 Protein denotes TGF-β
T26335 23246-23256 Gene_expression denotes expression true
T26336 23246-23256 Gene_expression denotes expression true
T18066 35795-35798 Protein denotes CD4
T18067 35809-35812 Protein denotes CD4
T18068 35890-35893 Protein denotes CD4
T18069 35979-35982 Protein denotes CD4
T18070 36177-36181 Positive_regulation denotes high
T18071 36182-36186 Protein denotes IL-4
T18072 36187-36196 Gene_expression denotes secretion
T22244 44335-44339 Protein denotes IL-4
T22245 44388-44392 Protein denotes IL-4
T22246 44409-44413 Protein denotes IL-4
T22247 44521-44524 Protein denotes CD3
T22248 44526-44529 Protein denotes CD4
T22249 44535-44539 Protein denotes CD25
T22250 44540-44550 Gene_expression denotes expression true
T22251 44540-44550 Gene_expression denotes expression true
T22252 44540-44550 Gene_expression denotes expression true
T22253 44567-44571 Protein denotes IL-4
T22254 44636-44640 Protein denotes IL-4
T20164 40294-40297 Protein denotes TAT
T20165 40327-40332 Protein denotes GATA3
T20166 40358-40363 Protein denotes GATA3
T20167 40365-40372 Negative_regulation denotes lacking
T20168 40377-40393 Entity denotes two zinc fingers
T20169 40457-40460 Protein denotes TAT
T20170 40631-40634 Protein denotes TAT
T19512 38876-38881 Protein denotes FOXP3
T19513 38934-38939 Protein denotes FOXP3
T19514 39026-39031 Protein denotes FOXP3
T19515 39081-39086 Protein denotes FOXP3
T19090 38323-38326 Protein denotes CD4
T19091 38328-38334 Protein denotes CD45RA
T19092 38357-38363 Protein denotes CD45RO
T19093 38364-38373 Negative_regulation denotes depletion
T19094 38484-38487 Protein denotes CD3
T19095 38519-38523 Protein denotes CD28
T19096 38562-38567 Protein denotes IL-12
T19097 38582-38586 Protein denotes IL-4
T19098 38630-38634 Protein denotes IL-4
T19099 38649-38654 Protein denotes IFN-γ
T19100 38669-38674 Protein denotes IL-12
T19101 38722-38727 Protein denotes TGF-β
T19102 38742-38747 Protein denotes IFN-γ
T19103 38762-38767 Protein denotes IL-12
T19104 38782-38786 Protein denotes IL-4
T19105 38843-38847 Protein denotes IL-2
T18320 36389-36394 Protein denotes FOXP3
T18321 36448-36453 Protein denotes FOXP3
T18322 36499-36504 Protein denotes EF-1α
T18323 36558-36563 Protein denotes GATA3
T18324 36977-36988 Protein denotes Ubiquitin C
T18325 37062-37068 Protein denotes Smad-7
T18326 37141-37147 Protein denotes TGF-ß1
T18327 37568-37579 Protein denotes ubiquitin C
T17765 35386-35396 Gene_expression denotes expressing
T17766 35397-35403 Protein denotes GATA-3
T17767 35425-35428 Protein denotes CD2
T17768 35451-35454 Protein denotes CD2
T17769 35455-35460 Protein denotes GATA3
T17770 35489-35492 Protein denotes CD2
T17771 35493-35498 Protein denotes GATA3
T20935 41610-41615 Protein denotes FOXP3
T20936 41616-41626 Gene_expression denotes expression true
T20937 41707-41711 Protein denotes CD25
T20938 41851-41856 Protein denotes FOXP3
T20939 42081-42086 Protein denotes CD152
T20940 42109-42113 Protein denotes PD-1
T20941 42135-42139 Protein denotes GITR
T20942 42162-42166 Protein denotes CD69
T20943 42191-42196 Protein denotes CD103
T20944 42220-42225 Protein denotes CD62L
T20945 42483-42486 Protein denotes CD3
T20946 42493-42496 Protein denotes CD4
T20947 42507-42511 Protein denotes CD25
T20948 42646-42649 Protein denotes CD4
T20949 42689-42692 Protein denotes CD2
T20950 42693-42698 Protein denotes GATA3
T20951 42737-42741 Protein denotes CD25
T20952 42753-42758 Protein denotes CD62L
T20953 42772-42775 Protein denotes CD4
T20954 42885-42890 Protein denotes Foxp3
T20955 42891-42901 Gene_expression denotes expression true
T20956 42975-42980 Protein denotes Foxp3
T20957 43050-43053 Protein denotes CD4
T8297 20048-20077 Negative_regulation denotes acts in vitro as an inhibitor
T8298 20087-20097 Gene_expression denotes expression
T8299 20150-20156 Positive_regulation denotes acting true
T8300 20220-20229 Regulation denotes dependent true
T8301 17604-17611 Negative_regulation denotes reduced
T8302 17623-17633 Gene_expression denotes expression
T8303 17604-17611 Negative_regulation denotes reduced
T8304 17644-17654 Gene_expression denotes expression
T8305 17687-17696 Negative_regulation denotes inhibited
T8306 17712-17721 Positive_regulation denotes induction
T8307 18249-18258 Positive_regulation denotes induction
T8308 17557-17562 Protein denotes TGF-β
T8309 17834-17838 Protein denotes IL-4
T8310 17910-17915 Protein denotes FOXP3
T8311 17968-17973 Protein denotes FOXP3
T8312 18061-18065 Protein denotes IL-4
T8313 18112-18117 Protein denotes FOXP3
T8314 18132-18137 Protein denotes FOXP3
T8315 18234-18239 Protein denotes TGF-β
T8316 18262-18267 Protein denotes FOXP3
T8317 18313-18317 Protein denotes CD25
T8318 18318-18323 Protein denotes FOXP3
T8319 18355-18360 Protein denotes FOXP3
T8320 18399-18402 Protein denotes CD4
T8321 18403-18409 Protein denotes CD45RA
T8322 18448-18453 Protein denotes FOXP3
T8323 18508-18512 Protein denotes CD25
T8324 18535-18540 Protein denotes TGF-β
T8325 18639-18644 Protein denotes TGF-β
T8326 18649-18653 Protein denotes IL-4
T8327 19296-19300 Protein denotes IL-4
T8328 19353-19358 Protein denotes FOXP3
T8329 17612-17616 Protein denotes CD25
T8330 19419-19423 Protein denotes IL-4
T8331 19448-19453 Protein denotes FOXP3
T8332 19509-19514 Protein denotes FOXP3
T8333 19597-19602 Protein denotes GATA3
T8334 19607-19612 Protein denotes FOXP3
T8335 19676-19680 Protein denotes IL-4
T8336 19685-19690 Protein denotes TGF-β
T8337 19709-19714 Protein denotes GATA3
T8338 19719-19724 Protein denotes FOXP3
T6488 14207-14212 Protein denotes TGF-β
T77 27-34 Negative_regulation denotes Inhibit
T78 994-1003 Positive_regulation denotes induction
T79 1024-1032 Positive_regulation denotes mediated
T80 1043-1050 Binding denotes binding
T81 1344-1350 Regulation denotes target
T82 1344-1350 Regulation denotes target
T83 42-49 Positive_regulation denotes Induced
T84 56-66 Gene_expression denotes Expression
T85 530-540 Regulation denotes regulating true
T86 545-555 Gene_expression denotes expression
T87 767-775 Negative_regulation denotes inhibits
T88 875-889 Gene_expression denotes overexpressing
T89 931-940 Negative_regulation denotes deficient
T90 979-987 Positive_regulation denotes mediated
T91 0-5 Protein denotes GATA3
T92 988-993 Protein denotes FOXP3
T93 1054-1059 Protein denotes GATA3
T94 1067-1072 Protein denotes FOXP3
T95 1245-1249 Protein denotes IL-4
T96 1351-1355 Protein denotes IL-4
T97 1359-1364 Protein denotes GATA3
T98 35-41 Protein denotes TGF-β1
T99 50-55 Protein denotes FOXP3
T100 1073-1081 Entity denotes promoter
T101 388-393 Protein denotes FOXP3
R621 T84 T83 themeOf Expression,Induced
R622 T86 T85 themeOf expression,regulating
R623 T89 T79 themeOf deficient,mediated
R624 T90 T89 themeOf mediated,deficient
R625 T92 T78 themeOf FOXP3,induction
R626 T93 T80 themeOf GATA3,binding
R627 T96 T81 themeOf IL-4,target
R628 T97 T82 themeOf GATA3,target
R629 T98 T83 causeOf TGF-β1,Induced
R630 T99 T84 themeOf FOXP3,Expression
R631 T100 T80 themeOf promoter,binding
R632 T100 T94 partOf promoter,FOXP3
R633 T101 T86 themeOf FOXP3,expression
R740 T926 T927 causeOf GATA3,induction
R741 T926 T929 causeOf GATA3,induction
R742 T926 T928 causeOf GATA3,induction
R743 T930 T928 themeOf IL-4,induction
R744 T931 T927 themeOf IL-5,induction
R745 T932 T929 themeOf IL-13,induction
R746 T933 T934 causeOf T-bet,modulates
R747 T935 T934 themeOf GATA3,modulates
R748 T939 T938 themeOf GATA3,Overexpression of a dominant negative form
R749 T940 T941 themeOf GATA3,blockade
R750 T943 T942 themeOf FOXP3,express
R751 T945 T944 themeOf CD25,express
R752 T947 T946 themeOf FOXP3,express
R753 T949 T950 themeOf FOXP3,expression
R754 T950 T948 themeOf expression,induce
R755 T953 T952 themeOf TGF-βRII,lacking
R756 T959 T960 causeOf GATA3,inhibits
R757 T961 T962 themeOf FOXP3,induction
R758 T962 T960 themeOf induction,inhibits
R759 T963 T964 causeOf IL-4,limits
R760 T964 T968 themeOf limits,mediated way
R761 T965 T966 themeOf FOXP3,expression
R762 T966 T964 themeOf expression,limits
R763 T967 T968 causeOf GATA3,mediated way
R764 T969 T970 themeOf GATA3,binds
R765 T970 T973 causeOf binds,prevents
R766 T971 T974 themeOf FOXP3,induction
R767 T972 T970 themeOf promoter,binds
R768 T972 T971 partOf promoter,FOXP3
R769 T974 T973 themeOf induction,prevents
R2316 T2910 T2921 themeOf expression,induced
R2317 T2912 T2911 themeOf express,inability
R2318 T2914 T2913 themeOf expression,lack
R2319 T2918 T2917 themeOf expression,low
R2320 T2920 T2919 themeOf expression,increase
R2321 T2923 T2922 themeOf expression,induced
R2322 T2927 T2926 themeOf expression,lacking
R2323 T2928 T2929 themeOf induction,failed
R2324 T2943 T2944 themeOf expression,restricted
R2326 T2956 T2946 themeOf FOXP3,induce
R2335 T2966 T2908 themeOf FOXP3,expression
R2336 T2967 T2909 themeOf FOXP3,express
R2337 T2968 T2912 themeOf FOXP3,express
R2338 T2970 T2914 themeOf FOXP3,expression
R2339 T2971 T2915 themeOf FOXP3,expressed
R2340 T2972 T2916 themeOf FOXP3,express
R2341 T2973 T2918 themeOf CD25,expression
R2342 T2974 T2919 causeOf TGF-β,increase
R2343 T2975 T2920 themeOf FOXP3,expression
R2344 T2976 T2923 themeOf FOXP3,expression
R2345 T2978 T2924 themeOf IL-4,secretion
R2346 T2979 T2925 themeOf IL-4,producing
R2347 T2980 T2932 themeOf FOXP3,express
R2348 T2981 T2927 themeOf FOXP3,expression
R2349 T2982 T2928 themeOf FOXP3,induction
R2350 T2984 T2930 themeOf IL-4,expression
R2351 T2985 T2931 themeOf FOXP3,expression
R2352 T2987 T2933 themeOf IL-4,expressing
R2353 T2990 T2942 themeOf FOXP3,expressed
R2354 T2990 T2943 themeOf FOXP3,expression
R2355 T2991 T2934 themeOf FOXP3,co-express
R2356 T2994 T2935 themeOf FOXP3,expressed
R2357 T2995 T2936 themeOf IL-4,lacking
R2358 T3001 T2945 themeOf FOXP3,mRNA expression
R2359 T3008 T2937 themeOf FOXP3,expressed
R2360 T3009 T2938 themeOf IL-4,secretion
R2361 T3010 T2939 themeOf FOXP3,expressing
R2362 T3011 T2940 themeOf FOXP3,expressed
R2363 T3011 T2941 themeOf FOXP3,inducible
R2364 T3015 T2928 causeOf TGF-β,induction
R3989 T5198 T5201 themeOf GATA3,expression
R3990 T5199 T5200 themeOf FOXP3,expression
R3991 T5200 T5202 themeOf expression,resolved
R3992 T5201 T5203 themeOf expression,resolved
R3993 T5205 T5204 themeOf express,lost
R3994 T5206 T5205 themeOf FOXP3,express
R4871 T6402 T6404 themeOf prevention,caused
R4872 T6403 T6402 themeOf expression,prevention
R4873 T6405 T6407 themeOf phosphorylation,affected
R4874 T6405 T6408 themeOf phosphorylation,affected
R4875 T6406 T6409 themeOf phosphorylation,affected
R4876 T6406 T6411 themeOf phosphorylation,affected
R4877 T6410 T6399 themeOf induction,inhibit
R4878 T6412 T6413 causeOf Increasing amounts,increase
R4879 T6412 T6414 causeOf Increasing amounts,decreased
R4880 T6416 T6417 themeOf expression,suppressive capacity
R4881 T6418 T6420 themeOf inhibit,altered
R4882 T6419 T6418 themeOf expression,inhibit
R4883 T6488 T6424 causeOf TGF-β,induction
R4894 T6423 T6422 themeOf expression,decrease
R4897 T6424 T6421 themeOf induction,repressed
R4898 T6425 T6424 themeOf expression,induction
R4899 T6427 T6426 causeOf absence,induced
R4901 T6429 T6428 themeOf expression,induce
R4904 T6434 T6421 causeOf IL-4,repressed
R4905 T6435 T6425 themeOf FOXP3,expression
R4906 T6436 T6426 themeOf GATA3,induced
R4908 T6437 T6427 themeOf IL-4,absence
R4910 T6438 T6428 causeOf IL-4,induce
R4911 T6439 T6429 themeOf FOXP3,expression
R4912 T6441 T6400 themeOf GATA3,induced
R4913 T6441 T6401 themeOf GATA3,induced
R4914 T6442 T6400 causeOf TGF-β,induced
R4915 T6443 T6401 causeOf IL-4,induced
R4916 T6444 T6402 causeOf IL-4,prevention
R4917 T6445 T6403 themeOf FOXP3,expression
R4918 T6446 T6405 themeOf SMAD2,phosphorylation
R4920 T6447 T6406 themeOf STAT6,phosphorylation
R4922 T6448 T6407 causeOf IL-4,affected
R4923 T6448 T6409 causeOf IL-4,affected
R4924 T6449 T6408 causeOf TGF-β,affected
R4925 T6449 T6411 causeOf TGF-β,affected
R4928 T6453 T6412 themeOf IL-4,Increasing amounts
R4930 T6454 T6413 themeOf GATA3,increase
R4931 T6455 T6414 themeOf FOXP3,decreased
R4932 T6457 T6415 themeOf CD25,express
R4934 T6459 T6458 equivalentTo rmIL-4,IL-4
R4936 T6462 T6399 causeOf IL-4,inhibit
R4941 T6473 T6410 causeOf TGF-β,induction
R4945 T6478 T6417 causeOf IL-4,suppressive capacity
R4946 T6479 T6416 themeOf FOXP3,expression
R4947 T6480 T6418 causeOf IL-4,inhibit
R4948 T6481 T6419 themeOf FOXP3,expression
R4950 T6482 T6423 themeOf FOXP3,expression
R4951 T6483 T6422 causeOf IL-4,decrease
R4952 T6484 T6410 themeOf FOXP3,induction
R6278 T8281 T8307 themeOf expression,induction
R6279 T8283 T8284 themeOf down-regulated,pronounced
R6280 T8290 T8289 themeOf co-express,demonstrated
R6281 T8290 T8292 themeOf co-express,demonstrated
R6282 T8291 T8280 themeOf expression,reduced
R6283 T8294 T8293 themeOf co-express,demonstrated
R6284 T8294 T8295 themeOf co-express,demonstrated
R6285 T8297 T8299 themeOf acts in vitro as an inhibitor,acting
R6286 T8298 T8297 themeOf expression,acts in vitro as an inhibitor
R6287 T8300 T8299 causeOf dependent,acting
R6288 T8302 T8301 themeOf expression,reduced
R6289 T8304 T8303 themeOf expression,reduced
R6290 T8306 T8305 themeOf induction,inhibited
R6291 T8307 T8282 themeOf induction,caused
R6292 T8315 T8307 causeOf TGF-β,induction
R6293 T8316 T8281 themeOf FOXP3,expression
R6294 T8323 T8283 themeOf CD25,down-regulated
R6295 T8324 T8283 causeOf TGF-β,down-regulated
R6296 T8326 T8284 causeOf IL-4,pronounced
R6297 T8329 T8291 themeOf CD25,expression
R6298 T8330 T8285 themeOf IL-4,producing
R6299 T8331 T8286 themeOf FOXP3,expressing
R6300 T8333 T8287 themeOf GATA3,expression
R6301 T8334 T8288 themeOf FOXP3,expression
R6302 T8335 T8289 causeOf IL-4,demonstrated
R6303 T8335 T8293 causeOf IL-4,demonstrated
R6304 T8336 T8292 causeOf TGF-β,demonstrated
R6305 T8336 T8295 causeOf TGF-β,demonstrated
R6306 T8337 T8290 themeOf GATA3,co-express
R6307 T8338 T8294 themeOf FOXP3,co-express
R6308 T8339 T8296 causeOf GATA3,repressing
R6309 T8340 T8302 themeOf IL-4,expression
R6310 T8341 T8296 themeOf FOXP3,repressing
R6311 T8342 T8297 causeOf IL-4,acts in vitro as an inhibitor
R6312 T8342 T8300 causeOf IL-4,dependent
R6313 T8343 T8298 themeOf FOXP3,expression
R6314 T8345 T8300 themeOf GATA3,dependent
R6315 T8346 T8304 themeOf CD25,expression
R6316 T8347 T8305 causeOf IL-4,inhibited
R6317 T8348 T8306 causeOf TGF-β,induction
R6318 T8349 T8306 themeOf FOXP3,induction
R6321 T8351 T8303 causeOf TGF-β,reduced
R8606 T11547 T11548 themeOf GATA3,expressing
R8607 T11550 T11549 themeOf GATA3,overexpressing
R8608 T11551 T11552 themeOf GATA3,binding
R8609 T11553 T11554 themeOf GATA3,binds
R8610 T11556 T11554 themeOf promoter,binds
R8611 T11556 T11555 partOf promoter,FOXP3
R8612 T11558 T11559 themeOf GATA3,binds
R8613 T11561 T11559 themeOf promoter region,binds
R8614 T11561 T11560 partOf promoter region,FOXP3
R8615 T11562 T11563 themeOf GATA3,binding
R8616 T11563 T11565 causeOf binding,repressing
R8617 T11564 T11563 themeOf FOXP3,binding
R8618 T11566 T11567 themeOf FOXP3,expression
R8619 T11567 T11565 themeOf expression,repressing
R9959 T13498 T13509 themeOf induction,inhibited
R9960 T13501 T13503 themeOf expression,low
R9961 T13502 T13504 themeOf expression,low
R9962 T13515 T13514 themeOf expression,inhibitors
R9963 T13517 T13516 themeOf expression,inhibits
R9964 T13519 T13518 themeOf induction,inhibit
R9965 T13519 T13521 themeOf induction,inhibit
R9966 T13522 T13523 causeOf express,inhibit
R9967 T13524 T13523 themeOf expression,inhibit
R9968 T13526 T13525 themeOf drop,contribute
R9969 T13527 T13526 themeOf expression,drop
R9970 T13530 T13529 themeOf expression,enhanced
R9971 T13533 T13532 themeOf expression,induce
R9972 T13535 T13534 themeOf expression,regulate
R9973 T13538 T13539 themeOf inhibition,mediated
R9986 T13571 T13570 themeOf binding,compete
R9987 T13574 T13573 themeOf expression,acts as an inhibitor
R9988 T13579 T13578 themeOf expression,repressing
R9996 T13593 T13553 themeOf T-bet,expression
R9997 T13593 T13575 themeOf T-bet,co-expression
R9998 T13593 T13499 themeOf T-bet,expression
R10001 T13595 T13570 causeOf GATA3,compete
R10002 T13596 T13569 themeOf AP-1,binding
R10003 T13597 T13571 themeOf NFAT,binding
R10004 T13598 T13572 themeOf FOXP3,negatively regulated
R10005 T13599 T13572 causeOf IL-4,negatively regulated
R10006 T13600 T13573 causeOf GATA3,acts as an inhibitor
R10007 T13600 T13576 themeOf GATA3,binding
R10008 T13600 T13577 causeOf GATA3,repressing
R10009 T13601 T13574 themeOf FOXP3,expression
R10010 T13603 T13578 causeOf Il-4,repressing
R10011 T13604 T13564 themeOf GATA3,expression
R10012 T13604 T13580 themeOf GATA3,co-expression
R10013 T13604 T13500 themeOf GATA3,expression
R10014 T13605 T13579 themeOf FOXP3,expression
R10015 T13607 T13503 causeOf TGF-β,low
R10016 T13607 T13504 causeOf TGF-β,low
R10017 T13609 T13501 themeOf GATA3,expression
R10018 T13610 T13502 themeOf T-bet,expression
R10019 T13611 T13505 themeOf IL-4,absence
R10022 T13613 T13506 themeOf IL-12,absence
R10023 T13614 T13569 themeOf promoter,binding
R10024 T13614 T13594 partOf promoter,FOXP3
R10025 T13614 T13571 themeOf promoter,binding
R10026 T13615 T13576 themeOf promoter,binding
R10027 T13615 T13602 partOf promoter,FOXP3
R10028 T13615 T13577 themeOf promoter,repressing
R10029 T13616 T13509 causeOf GATA3,inhibited
R10030 T13617 T13507 themeOf TGF-β,absence
R10031 T13618 T13508 themeOf FOXP3,expression
R10032 T13619 T13510 themeOf FOXP3,expressing
R10033 T13620 T13511 themeOf GATA3,up-regulated
R10034 T13621 T13512 themeOf T-bet,up-regulated
R10035 T13622 T13513 themeOf FOXP3,promoting
R10036 T13623 T13513 causeOf CTLA-4,promoting
R10037 T13623 T13514 causeOf CTLA-4,inhibitors
R10038 T13624 T13515 themeOf GATA3,expression
R10039 T13625 T13516 causeOf GATA3,inhibits
R10040 T13626 T13517 themeOf FOXP3,expression
R10041 T13628 T13518 causeOf IL-4,inhibit
R10042 T13629 T13521 causeOf IL-13,inhibit
R10043 T13630 T13519 themeOf FOXP3,induction
R10044 T13640 T13522 themeOf CD25,express
R10045 T13642 T13524 themeOf Foxp3,expression
R10046 T13643 T13527 themeOf Foxp3,expression
R10047 T13645 T13528 themeOf IL-17,producing
R10048 T13656 T13529 causeOf IL-4,enhanced
R10049 T13656 T13532 causeOf IL-4,induce
R10050 T13657 T13530 themeOf FOXP3,expression
R10051 T13658 T13533 themeOf GATA3,expression
R10052 T13659 T13520 themeOf FOXP3,up-regulate
R10053 T13661 T13535 themeOf FOXP3,expression
R10054 T13662 T13536 causeOf IL-13,reduce
R10055 T13662 T13537 causeOf IL-13,induce
R10056 T13663 T13536 themeOf FOXP3,reduce
R10057 T13664 T13537 themeOf GATA3,induce
R10058 T13665 T13538 causeOf IL-4,inhibition
R10059 T13666 T13538 themeOf FOXP3,inhibition
R10063 T13670 T13531 themeOf FOXP3,induction
R10069 T13680 T13542 themeOf FOXP3,up-regulate
R10073 T13691 T13542 causeOf TGF-β,up-regulate
R15290 T20437 T20438 themeOf FOXP3,expression
R18703 T25121 T25118 themeOf Induction,Effect
R18704 T25128 T25127 themeOf IL-4,increasing
R18705 T25137 T25136 themeOf SMAD2,phosphorylated
R18706 T25138 T25135 themeOf STAT6,phosphorylated
R18707 T25141 T25143 themeOf GATA3,staining
R18708 T25142 T25144 themeOf FOXP3,staining
R19810 T26903 T26904 causeOf GATA3,Represses
R19811 T26906 T26904 themeOf Promoter,Represses
R19812 T26906 T26905 partOf Promoter,FOXP3
R19813 T26913 T26914 themeOf GATA3,expression
R19814 T26921 T26920 themeOf GATA3,amounts
R19815 T26923 T26924 themeOf GATA3,binding
R19816 T26926 T26924 themeOf promoter,binding
R19817 T26926 T26925 partOf promoter,FOXP3
R8581 T11500 T11501 causeOf GATA3,Represses
R8582 T11503 T11501 themeOf Promoter,Represses
R8583 T11503 T11502 partOf Promoter,FOXP3
R8584 T11504 T11505 causeOf GATA3,repression
R8585 T11506 T11505 themeOf FOXP3,repression
R8586 T11509 T11510 causeOf GATA-binding site,play an important role
R8587 T11509 T11507 partOf GATA-binding site,FOXP3
R8588 T11511 T11512 themeOf FOXP3,regulation
R8589 T11512 T11510 themeOf regulation,play an important role
R8590 T11516 T11515 themeOf GATA3,express
R8591 T11518 T11521 themeOf promoter,active
R8592 T11518 T11517 partOf promoter,FOXP3
R8593 T11519 T11520 themeOf GATA3,expressing
R8594 T11523 T11521 causeOf express,active
R8595 T11524 T11523 themeOf GATA3,express
R8596 T11526 T11525 themeOf GATA3,Overexpression
R8597 T11529 T11528 themeOf GATA3-binding site,deleting
R8598 T11529 T11527 partOf GATA3-binding site,FOXP3
R8599 T11536 T11535 causeOf GATA3,repressor
R8600 T11538 T11535 themeOf promoter,repressor
R8601 T11538 T11537 partOf promoter,FOXP3
R8602 T11539 T11540 themeOf GATA3,binds
R8603 T11542 T11540 themeOf promoter,binds
R8604 T11542 T11541 partOf promoter,FOXP3
R8605 T11545 T11546 themeOf GATA3,binding
R14158 T18785 T18786 themeOf FOXP3,expression
R17841 T23802 T23801 themeOf FOXP3,Express
R17842 T23803 T23804 themeOf FOXP3,expression
R17843 T23806 T23807 themeOf FOXP3,expression
R17593 T23447 T23448 themeOf FOXP3,Expression
R17594 T23448 T23446 themeOf Expression,Induce
R3971 T5162 T5163 themeOf FOXP3,expression
R3972 T5163 T5161 themeOf expression,induce
R3973 T5166 T5165 themeOf FOXP3,expression
R3974 T5167 T5164 themeOf GATA3,expression
R3975 T5172 T5175 themeOf FOXP3,expression
R3976 T5173 T5174 themeOf GATA3,expression
R3977 T5176 T5177 themeOf FOXP3,mRNA expression
R3978 T5177 T5178 themeOf mRNA expression,increased
R3979 T5179 T5182 themeOf GATA3,expression
R3980 T5180 T5181 themeOf FOXP3,expression
R3981 T5183 T5184 themeOf expressing,more
R3982 T5185 T5183 themeOf FOXP3,expressing
R3983 T5186 T5187 themeOf expressed,low
R3984 T5188 T5186 themeOf GATA3,expressed
R3985 T5190 T5194 themeOf CD103,expression
R3986 T5191 T5197 themeOf CTLA-4,expression
R3987 T5192 T5196 themeOf GITR,expression
R3988 T5193 T5195 themeOf PD-1,expression
R735 T920 T922 causeOf interleukin 4,induced
R736 T920 T923 causeOf interleukin 4,induced
R737 T921 T920 equivalentTo IL-4,interleukin 4
R738 T924 T922 themeOf STAT6,induced
R739 T925 T923 themeOf GATA3,induced
R7379 T9753 T9754 causeOf GATA3,Negative Regulator
R7380 T9755 T9756 themeOf FOXP3,Expression
R7381 T9756 T9754 themeOf Expression,Negative Regulator
R7387 T9764 T9763 causeOf GATA3,role
R7389 T9766 T9765 themeOf FOXP3,repressing
R7390 T9768 T9769 causeOf GATA3,inhibiting
R7391 T9770 T9771 themeOf STAT4,expression
R7392 T9771 T9769 themeOf expression,inhibiting
R7393 T9773 T9774 causeOf GATA3,inhibit
R7394 T9775 T9776 themeOf FOXP3,induction
R7395 T9776 T9774 themeOf induction,inhibit
R7396 T9780 T9779 themeOf DNA-binding domain,lacking
R7397 T9780 T9778 partOf DNA-binding domain,GATA3
R7398 T9789 T9790 causeOf TAT-GATA3,reduced
R7399 T9791 T9792 themeOf FOXP3,expression
R7400 T9792 T9790 themeOf expression,reduced
R7401 T9793 T9794 themeOf DNA-binding domain,truncated
R7402 T9793 T9795 partOf DNA-binding domain,GATA3
R7403 T9794 T9796 causeOf truncated,affect
R7404 T9797 T9798 themeOf FOXP3,expression
R7405 T9798 T9796 themeOf expression,affect
R7406 T9800 T9799 causeOf GATA3,inhibitory effect
R7407 T9801 T9799 themeOf FOXP3,inhibitory effect
R7408 T9803 T9802 themeOf GATA3,overexpressing
R18701 T25119 T25118 causeOf IL-4,Effect
R18702 T25120 T25121 themeOf FOXP3,Induction
R7382 T9757 T9758 themeOf FOXP3,expression
R7383 T9758 T9759 themeOf expression,decreased
R7384 T9760 T9761 themeOf GATA3,expression
R7385 T9761 T9759 causeOf expression,decreased
R7386 T9761 T9762 themeOf expression,high
R7388 T9765 T9763 themeOf repressing,role
R7409 T9811 T9810 causeOf GATA3,effect
R7410 T9816 T9817 themeOf Foxp3,expression
R7411 T9817 T9810 themeOf expression,effect
R7412 T9821 T9822 causeOf TGF-β,up-regulated
R7413 T9823 T9822 themeOf Foxp3,up-regulated
R7414 T9825 T9826 causeOf GATA3,reduced
R7415 T9827 T9828 themeOf Foxp3,expression
R7416 T9828 T9826 themeOf expression,reduced
R7417 T9828 T9829 themeOf expression,activated
R7418 T9830 T9829 causeOf TGF-β,activated
R7419 T9832 T9831 themeOf TGF-β,produced
R7420 T9833 T9834 themeOf Smad7,expressed
R7421 T9836 T9837 causeOf IL-4,induced
R7422 T9838 T9837 themeOf GATA3,induced
R18438 T24681 T24682 themeOf FOXP3,Induction
R18439 T24689 T24692 themeOf FOXP3,expression
R18440 T24690 T24691 themeOf GATA3,expression
R18441 T24693 T24695 themeOf GATA3,staining
R18442 T24694 T24696 themeOf FOXP3,staining
R18053 T24098 T24099 themeOf IL-4,Producing
R18054 T24101 T24100 themeOf FOXP3,Lack
R18055 T24102 T24104 themeOf FOXP3,expression
R18056 T24103 T24105 themeOf IL-4,expression
R18057 T24107 T24109 themeOf CD45RO,expression
R18058 T24108 T24110 themeOf CD25,expression
R18059 T24118 T24119 themeOf IL-4,secreting
R18060 T24123 T24121 themeOf FOXP3,stained
R18061 T24124 T24122 themeOf IL-4,stained
R19447 T26320 T26319 themeOf GATA3,presence
R19448 T26324 T26325 themeOf FOXP3,expression
R19449 T26333 T26335 themeOf SMAD7,expression
R19450 T26334 T26336 themeOf TGF-β,expression
R13647 T18071 T18072 themeOf IL-4,secretion
R13648 T18072 T18070 themeOf secretion,high
R16567 T22247 T22252 themeOf CD3,expression
R16568 T22248 T22251 themeOf CD4,expression
R16569 T22249 T22250 themeOf CD25,expression
R15109 T20168 T20167 themeOf two zinc fingers,lacking
R15110 T20168 T20166 partOf two zinc fingers,GATA3
R14370 T19092 T19093 themeOf CD45RO,depletion
R13417 T17766 T17765 themeOf GATA-3,expressing
R15617 T20935 T20936 themeOf FOXP3,expression
R15618 T20954 T20955 themeOf Foxp3,expression
R9984 T13565 T13566 themeOf expression,low
R9985 T13569 T13568 themeOf binding,compete
R9989 T13581 T13498 themeOf FOXP3,induction
R9990 T13585 T13560 themeOf GATA3,express
R9991 T13586 T13561 themeOf GATA3,express
R9992 T13587 T13562 themeOf GATA3,bound
R9993 T13588 T13563 themeOf GATA3,binds
R9994 T13590 T13565 themeOf GATA3,expression
R9995 T13592 T13567 causeOf GATA3,inhibit
R9999 T13594 T13567 themeOf FOXP3,inhibit
R10000 T13595 T13568 causeOf GATA3,compete
R6319 T8351 T8280 causeOf TGF-β,reduced
R6320 T8351 T8301 causeOf TGF-β,reduced
R9974 T13540 T13541 causeOf inducing,repressed
R9975 T13543 T13541 themeOf mediated,repressed
R9976 T13544 T13543 themeOf expression,mediated
R9977 T13546 T13545 themeOf expressing,high
R9978 T13549 T13548 themeOf induction,inhibited
R9979 T13551 T13552 causeOf overexpression,failed
R9980 T13554 T13552 themeOf induce,failed
R9981 T13555 T13554 themeOf expression,induce
R9982 T13558 T13557 themeOf expression,repressed
R9983 T13559 T13557 causeOf binding,repressed
R10020 T13612 T13559 themeOf promoter region,binding
R10021 T13612 T13583 partOf promoter region,FOXP3
R10060 T13667 T13539 causeOf GATA3,mediated
R10061 T13668 T13540 causeOf GATA3,inducing
R10062 T13669 T13540 themeOf IL-4,inducing
R10064 T13671 T13544 themeOf FOXP3,expression
R10065 T13676 T13546 themeOf GATA3,expressing
R10066 T13677 T13547 themeOf FOXP3,absence
R10067 T13678 T13548 causeOf GATA3,inhibited
R10068 T13679 T13549 themeOf FOXP3,induction
R10070 T13682 T13550 themeOf GATA3,expressing
R10071 T13685 T13551 themeOf GATA3,overexpression
R10072 T13686 T13555 themeOf FOXP3,expression
R10074 T13693 T13556 themeOf GATA3,overexpression
R10075 T13697 T13696 equivalentTo ROG,Repressor of GATA3
R10076 T13700 T13559 themeOf GATA3,binding
R10077 T13701 T13558 themeOf FOXP3,expression
R10078 T13702 T13519 causeOf TGF-β,induction
R10079 T13703 T13543 causeOf TGF-β,mediated
R19187 T25865 T25863 themeOf FOXP3,staining
R19188 T25878 T25862 themeOf GATA3,staining
R2312 T2901 T2900 themeOf expression,increase
R2313 T2905 T2904 themeOf induction,prevent
R2314 T2906 T2907 causeOf neutralization,rescue
R2315 T2908 T2907 themeOf expression,rescue
R2325 T2947 T2899 themeOf FOXP3,Induction
R2327 T2957 T2946 causeOf TGF-β,induce
R2328 T2958 T2910 themeOf FOXP3,expression
R2329 T2959 T2901 themeOf FOXP3,expression
R2330 T2960 T2902 themeOf FOXP3,up-regulate
R2331 T2961 T2903 themeOf IL-4,produce
R2332 T2961 T2904 causeOf IL-4,prevent
R2333 T2963 T2905 themeOf FOXP3,induction
R2334 T2964 T2906 themeOf IL-4,neutralization
R39 T102 T87 causeOf interleukin 4,inhibits
R40 T103 T102 equivalentTo IL-4,interleukin 4
R41 T104 T87 themeOf FOXP3,inhibits
R42 T105 T88 themeOf GATA-3,overexpressing
R43 T106 T90 causeOf transforming growth factor (TGF)-β,mediated
R618 T78 T90 themeOf induction,mediated
R619 T80 T79 causeOf binding,mediated
R620 T83 T77 themeOf Induced,Inhibit

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T339 0-5 P23771 denotes GATA3
T340 50-55 Q9BZS1 denotes FOXP3
T341 388-393 Q9BZS1 denotes FOXP3
T342 708-721 P05112 denotes interleukin 4
T343 723-727 P05112 denotes IL-4
T344 776-781 Q9BZS1 denotes FOXP3
T345 890-896 P23771 denotes GATA-3
T346 988-993 Q9BZS1 denotes FOXP3
T347 1054-1059 P23771 denotes GATA3
T348 1067-1072 Q9BZS1 denotes FOXP3
T349 1245-1249 P05112 denotes IL-4
T350 1351-1355 P05112 denotes IL-4
T351 1359-1364 P23771 denotes GATA3
T1583 3496-3509 P05112 denotes interleukin 4
T1584 3511-3515 P05112 denotes IL-4
T1585 3525-3530 P42226 denotes STAT6
T1586 3535-3540 P23771 denotes GATA3
T1587 3620-3625 P23771 denotes GATA3
T1588 3713-3717 P05112 denotes IL-4
T1589 3719-3723 P05113 denotes IL-5
T1590 3728-3733 P35225 denotes IL-13
T1591 3848-3853 Q9UL17 denotes T-bet
T1592 3873-3878 P23771 denotes GATA3
T1593 4077-4082 P23771 denotes GATA3
T1594 4177-4181 P05112 denotes IL-4
T1595 4297-4302 P23771 denotes GATA3
T1596 4344-4349 P23771 denotes GATA3
T1597 4798-4803 Q9BZS1 denotes FOXP3
T1598 4831-4835 P01589 denotes CD25
T1599 5106-5111 Q9BZS1 denotes FOXP3
T1600 5234-5239 Q9BZS1 denotes FOXP3
T1601 5356-5361 P01137 denotes TGF-β
T1602 5649-5654 P01137 denotes TGF-β
T1603 5833-5838 P23771 denotes GATA3
T1604 5843-5848 Q9BZS1 denotes FOXP3
T1605 5901-5906 Q9UL17 denotes T-bet
T1606 5911-5916 P23771 denotes GATA3
T1607 5977-5982 P23771 denotes GATA3
T1608 5992-5997 Q9BZS1 denotes FOXP3
T1609 6017-6021 P05112 denotes IL-4
T1610 6029-6034 Q9BZS1 denotes FOXP3
T1611 6051-6056 P23771 denotes GATA3
T1612 6116-6121 P23771 denotes GATA3
T1613 6144-6149 Q9BZS1 denotes FOXP3
T3595 6284-6289 Q9BZS1 denotes FOXP3
T3596 6337-6342 Q9BZS1 denotes FOXP3
T3597 6485-6488 P01730 denotes CD4
T3598 6532-6537 Q9BZS1 denotes FOXP3
T3599 6562-6567 Q9BZS1 denotes FOXP3
T3600 6660-6665 Q9BZS1 denotes FOXP3
T3601 6731-6735 P01589 denotes CD25
T3602 6745-6748 P01730 denotes CD4
T3603 6757-6763 P08575 denotes CD45RA
T3604 6774-6780 P08575 denotes CD45RO
T3605 6923-6926 P01730 denotes CD4
T3606 6927-6931 P01589 denotes CD25
T3607 6934-6940 P08575 denotes CD45RA
T3608 6943-6949 P08575 denotes CD45RO
T3609 6956-6959 P01730 denotes CD4
T3610 6960-6966 P08575 denotes CD45RO
T3611 6967-6971 P01589 denotes CD25
T3612 6978-6982 P00519 denotes able
T3613 7007-7012 Q9BZS1 denotes FOXP3
T3614 7068-7073 P01137 denotes TGF-β
T3615 7194-7199 Q9BZS1 denotes FOXP3
T3616 7282-7287 Q9BZS1 denotes FOXP3
T3617 7988-7992 P05112 denotes IL-4
T3618 8034-8039 P01137 denotes TGF-β
T3619 8067-8072 Q9BZS1 denotes FOXP3
T3620 8115-8119 P05112 denotes IL-4
T3621 8136-8140 P05112 denotes IL-4
T3622 8165-8170 Q9BZS1 denotes FOXP3
T3623 8308-8313 Q9BZS1 denotes FOXP3
T3624 8365-8370 Q9BZS1 denotes FOXP3
T3625 8448-8453 Q9BZS1 denotes FOXP3
T3626 8505-8510 Q9BZS1 denotes FOXP3
T3627 8594-8599 Q9BZS1 denotes FOXP3
T3628 8613-8617 P01589 denotes CD25
T3629 8659-8664 P01137 denotes TGF-β
T3630 8694-8699 Q9BZS1 denotes FOXP3
T3631 8756-8761 Q9BZS1 denotes FOXP3
T3632 8877-8881 P05112 denotes IL-4
T3633 8899-8903 P05112 denotes IL-4
T3634 8951-8956 Q9BZS1 denotes FOXP3
T3635 8993-8998 P01137 denotes TGF-β
T3636 9008-9013 Q9BZS1 denotes FOXP3
T3637 9100-9104 P05112 denotes IL-4
T3638 9148-9152 P05112 denotes IL-4
T3639 9157-9162 Q9BZS1 denotes FOXP3
T3640 9183-9186 P01730 denotes CD4
T3641 9256-9260 P05112 denotes IL-4
T3642 9303-9309 P08575 denotes CD45RO
T3643 9310-9314 P01589 denotes CD25
T3644 9348-9353 Q9BZS1 denotes FOXP3
T3645 9392-9398 P08575 denotes CD45RO
T3646 9399-9403 P01589 denotes CD25
T3647 9432-9437 Q9BZS1 denotes FOXP3
T3648 9453-9457 P05112 denotes IL-4
T3649 9528-9532 P05112 denotes IL-4
T3650 9567-9572 Q9BZS1 denotes FOXP3
T3651 9609-9613 P05112 denotes IL-4
T3652 9642-9647 Q9BZS1 denotes FOXP3
T3653 9673-9679 P08575 denotes CD45RO
T3654 9680-9684 P01589 denotes CD25
T3655 9687-9693 P08575 denotes CD45RO
T3656 9694-9698 P01589 denotes CD25
T3657 9705-9711 P08575 denotes CD45RO
T3658 9712-9716 P01589 denotes CD25
T3659 9822-9827 Q9BZS1 denotes FOXP3
T3660 9866-9870 P05112 denotes IL-4
T3661 9941-9946 Q9BZS1 denotes FOXP3
T3662 10055-10060 Q9BZS1 denotes FOXP3
T5531 11403-11408 Q9BZS1 denotes FOXP3
T5532 11413-11418 P23771 denotes GATA3
T5533 11517-11522 Q9BZS1 denotes FOXP3
T5534 11665-11670 Q9BZS1 denotes FOXP3
T5535 11675-11680 P23771 denotes GATA3
T5536 11717-11720 P01730 denotes CD4
T5537 11753-11757 P05112 denotes IL-4
T5538 11764-11769 P01579 denotes IFN-γ
T5539 11780-11785 P29460 denotes IL-12
T5540 11780-11785 P29459 denotes IL-12
T5541 11864-11869 Q9BZS1 denotes FOXP3
T5542 11874-11879 P23771 denotes GATA3
T5543 12039-12044 Q9BZS1 denotes FOXP3
T5544 12127-12132 P23771 denotes GATA3
T5545 12137-12142 Q9BZS1 denotes FOXP3
T5546 12373-12378 Q9BZS1 denotes FOXP3
T5547 12427-12432 P23771 denotes GATA3
T5548 12703-12706 P07766 denotes CD3
T5549 12703-12706 P04234 denotes CD3
T5550 12703-12706 P09693 denotes CD3
T5551 12703-12706 P20963 denotes CD3
T5552 12894-12899 P23771 denotes GATA3
T5553 12904-12909 Q9BZS1 denotes FOXP3
T5554 13110-13115 Q9BZS1 denotes FOXP3
T6969 14105-14108 P04234 denotes CD3
T6970 14105-14108 P20963 denotes CD3
T6971 14105-14108 P07766 denotes CD3
T6972 14105-14108 P09693 denotes CD3
T6973 14109-14113 P10747 denotes CD28
T6974 14133-14138 P01137 denotes TGF-β
T6975 14146-14150 P05112 denotes IL-4
T6976 14176-14180 P05112 denotes IL-4
T6977 14207-14212 P01137 denotes TGF-β
T6978 14235-14240 Q9BZS1 denotes FOXP3
T6979 14327-14332 P23771 denotes GATA3
T6980 14369-14373 P05112 denotes IL-4
T6981 14441-14445 P05112 denotes IL-4
T6982 14450-14454 P00519 denotes able
T6983 14476-14481 Q9BZS1 denotes FOXP3
T6984 14503-14508 P23771 denotes GATA3
T6985 14545-14550 P01137 denotes TGF-β
T6986 14559-14563 P05112 denotes IL-4
T6987 14602-14606 P05112 denotes IL-4
T6988 14630-14635 Q9BZS1 denotes FOXP3
T6989 14737-14742 Q15796 denotes SMAD2
T6990 14746-14751 P42226 denotes STAT6
T6991 14788-14792 P05112 denotes IL-4
T6992 14800-14805 P01137 denotes TGF-β
T6993 14831-14835 P05112 denotes IL-4
T6994 14847-14852 P01137 denotes TGF-β
T6995 14937-14941 P05112 denotes IL-4
T6996 14965-14970 P23771 denotes GATA3
T6997 14980-14985 Q9BZS1 denotes FOXP3
T6998 15079-15083 P05112 denotes IL-4
T6999 15274-15278 P01589 denotes CD25
T7000 15319-15323 P05112 denotes IL-4
T7001 15343-15347 P05112 denotes IL-4
T7002 15500-15503 P01730 denotes CD4
T7003 15504-15508 P01589 denotes CD25
T7004 15514-15519 Q9BZS1 denotes FOXP3
T7005 15663-15667 P05112 denotes IL-4
T7006 15703-15706 P01730 denotes CD4
T7007 15707-15711 P01589 denotes CD25
T7008 15736-15741 Q9BZS1 denotes Foxp3
T7009 15863-15866 P01730 denotes CD4
T7010 15867-15871 P01589 denotes CD25
T7011 15903-15906 P01730 denotes CD4
T7012 15907-15911 P01589 denotes CD25
T7013 15937-15941 P05112 denotes IL-4
T7014 16067-16071 P05112 denotes IL-4
T7015 16149-16153 P01589 denotes CD25
T7016 16180-16184 P05112 denotes IL-4
T7017 16198-16203 Q9BZS1 denotes FOXP3
T7018 16273-16277 P05112 denotes IL-4
T7019 16296-16301 Q9BZS1 denotes FOXP3
T7020 16438-16443 Q9BZS1 denotes FOXP3
T7021 16460-16464 P05112 denotes IL-4
T8707 17557-17562 P01137 denotes TGF-β
T8708 17598-17603 P01137 denotes TGF-β
T8709 17612-17616 P01589 denotes CD25
T8710 17618-17622 P05112 denotes IL-4
T8711 17639-17643 P01589 denotes CD25
T8712 17668-17672 P05112 denotes IL-4
T8713 17697-17702 P01137 denotes TGF-β
T8714 17725-17730 Q9BZS1 denotes FOXP3
T8715 17762-17767 Q9BZS1 denotes FOXP3
T8716 17834-17838 P05112 denotes IL-4
T8717 17910-17915 Q9BZS1 denotes FOXP3
T8718 17945-17953 Q04864 denotes relative
T8719 17968-17973 Q9BZS1 denotes FOXP3
T8720 18061-18065 P05112 denotes IL-4
T8721 18112-18117 Q9BZS1 denotes FOXP3
T8722 18132-18137 Q9BZS1 denotes FOXP3
T8723 18234-18239 P01137 denotes TGF-β
T8724 18262-18267 Q9BZS1 denotes FOXP3
T8725 18313-18317 P01589 denotes CD25
T8726 18318-18323 Q9BZS1 denotes FOXP3
T8727 18355-18360 Q9BZS1 denotes FOXP3
T8728 18399-18402 P01730 denotes CD4
T8729 18403-18409 P08575 denotes CD45RA
T8730 18448-18453 Q9BZS1 denotes FOXP3
T8731 18508-18512 P01589 denotes CD25
T8732 18535-18542 P01137 denotes TGF-β–t
T8733 18639-18644 P01137 denotes TGF-β
T8734 18649-18653 P05112 denotes IL-4
T8735 19296-19300 P05112 denotes IL-4
T8736 19353-19358 Q9BZS1 denotes FOXP3
T8737 19419-19423 P05112 denotes IL-4
T8738 19448-19453 Q9BZS1 denotes FOXP3
T8739 19509-19514 Q9BZS1 denotes FOXP3
T8740 19597-19602 P23771 denotes GATA3
T8741 19607-19612 Q9BZS1 denotes FOXP3
T8742 19676-19680 P05112 denotes IL-4
T8743 19685-19690 P01137 denotes TGF-β
T8744 19709-19714 P23771 denotes GATA3
T8745 19719-19724 Q9BZS1 denotes FOXP3
T8746 19950-19955 P23771 denotes GATA3
T8747 20010-20015 Q9BZS1 denotes FOXP3
T8748 20043-20047 P05112 denotes IL-4
T8749 20081-20086 Q9BZS1 denotes FOXP3
T8750 20124-20129 P01137 denotes TGF-β
T8751 20214-20219 P23771 denotes GATA3
T10245 20242-20247 P23771 denotes GATA3
T10246 20275-20280 Q9BZS1 denotes FOXP3
T10247 20292-20297 Q9BZS1 denotes FOXP3
T10248 20324-20329 P23771 denotes GATA3
T10249 20398-20403 P23771 denotes GATA3
T10250 20418-20423 Q9BZS1 denotes FOXP3
T10251 20433-20438 P23771 denotes GATA3
T10252 20488-20493 P23771 denotes GATA3
T10253 20583-20588 Q14765 denotes STAT4
T10254 20622-20627 P23771 denotes GATA3
T10255 20692-20697 P23771 denotes GATA3
T10256 20719-20724 Q9BZS1 denotes FOXP3
T10257 20750-20755 P23771 denotes GATA3
T10258 20771-20776 P23771 denotes GATA3
T10259 20825-20828 P01730 denotes CD4
T10260 20829-20835 P08575 denotes CD45RA
T10261 20853-20856 P04608 denotes TAT
T10262 20853-20856 Q8UMQ1 denotes TAT
T10263 20853-20856 P04610 denotes TAT
T10264 20853-20856 P04605 denotes TAT
T10265 20888-20893 P23771 denotes GATA3
T10266 20958-20961 P09693 denotes CD3
T10267 20958-20961 P04234 denotes CD3
T10268 20958-20961 P07766 denotes CD3
T10269 20958-20961 P20963 denotes CD3
T10270 20962-20966 P10747 denotes CD28
T10271 20997-21002 P01137 denotes TGF-β
T10272 21004-21007 P04608 denotes TAT
T10273 21004-21007 P04605 denotes TAT
T10274 21004-21007 Q8UMQ1 denotes TAT
T10275 21004-21007 P04610 denotes TAT
T10276 21008-21013 P23771 denotes GATA3
T10277 21096-21099 P01730 denotes CD4
T10278 21135-21138 P04605 denotes TAT
T10279 21135-21138 P04608 denotes TAT
T10280 21135-21138 P04610 denotes TAT
T10281 21135-21138 Q8UMQ1 denotes TAT
T10282 21139-21144 P23771 denotes GATA3
T10283 21153-21158 Q9BZS1 denotes FOXP3
T10284 21246-21249 P04605 denotes TAT
T10285 21246-21249 P04608 denotes TAT
T10286 21246-21249 P04610 denotes TAT
T10287 21246-21249 Q8UMQ1 denotes TAT
T10288 21255-21260 P23771 denotes GATA3
T10289 21277-21282 Q9BZS1 denotes FOXP3
T10290 21407-21412 P23771 denotes GATA3
T10291 21416-21421 Q9BZS1 denotes FOXP3
T10292 21480-21485 P23771 denotes GATA3
T10293 21511-21514 P06729 denotes CD2
T10294 21549-21554 P23771 denotes GATA3
T10295 21587-21590 P01730 denotes CD4
T10296 21632-21637 P23771 denotes GATA3
T10297 21803-21808 P23771 denotes GATA3
T10298 21819-21822 P01730 denotes CD4
T10299 21823-21828 P14151 denotes CD62L
T10300 21829-21833 P01589 denotes CD25
T10301 21871-21874 E2RVI8 denotes OVA
T10302 21905-21910 P01137 denotes TGF-β
T10303 21916-21921 Q9BZS1 denotes Foxp3
T10304 21967-21970 P01730 denotes CD4
T10305 21971-21975 P01589 denotes CD25
T10306 21988-21993 Q9BZS1 denotes Foxp3
T10307 22049-22054 P01137 denotes TGF-β
T10308 22081-22086 Q9BZS1 denotes Foxp3
T10309 22155-22158 P06729 denotes CD2
T10310 22206-22211 Q9BZS1 denotes Foxp3
T10311 22243-22248 P01137 denotes TGF-β
T10312 22253-22256 E2RVI8 denotes OVA
T10313 22307-22312 P01137 denotes TGF-β
T10314 22423-22428 P01137 denotes TGF-β
T10315 23382-23386 P05112 denotes IL-4
T10316 23395-23400 P23771 denotes GATA3
T12017 23457-23462 P23771 denotes GATA3
T12018 23477-23482 Q9BZS1 denotes FOXP3
T12019 23534-23539 P23771 denotes GATA3
T12020 23569-23574 Q9BZS1 denotes FOXP3
T12021 23586-23591 Q9BZS1 denotes FOXP3
T12022 23648-23653 P23771 denotes GATA3
T12023 23882-23887 Q9BZS1 denotes FOXP3
T12024 23958-23963 Q9BZS1 denotes FOXP3
T12025 24018-24021 P01730 denotes CD4
T12026 24134-24139 P23771 denotes GATA3
T12027 24166-24171 Q9BZS1 denotes FOXP3
T12028 24226-24231 P23771 denotes GATA3
T12029 24345-24348 P01730 denotes CD4
T12030 24388-24393 P23771 denotes GATA3
T12031 24425-24430 P23771 denotes GATA3
T12032 24459-24469 P08659 denotes luciferase
T12033 24486-24491 Q9BZS1 denotes FOXP3
T12034 24586-24591 P23771 denotes GATA3
T12035 24648-24653 P23771 denotes GATA3
T12036 24692-24702 P08659 denotes luciferase
T12037 24732-24735 P01730 denotes CD4
T12038 24736-24742 P08575 denotes CD45RO
T12039 24798-24803 P23771 denotes GATA3
T12040 24806-24809 P01730 denotes CD4
T12041 24810-24816 P08575 denotes CD45RA
T12042 24861-24866 P23771 denotes GATA3
T12043 24874-24879 Q9BZS1 denotes FOXP3
T12044 24914-24919 P23771 denotes GATA3
T12045 24942-24947 Q9BZS1 denotes FOXP3
T12046 25037-25042 P23771 denotes GATA3
T12047 25150-25155 P23771 denotes GATA3
T12048 25168-25173 Q9BZS1 denotes FOXP3
T12049 25227-25232 P23771 denotes GATA3
T12050 25268-25273 P23771 denotes GATA3
T12051 25323-25328 P23771 denotes GATA3
T12052 25429-25434 P23771 denotes GATA3
T12053 25456-25461 P23771 denotes GATA3
T12054 25535-25540 P23771 denotes GATA3
T12055 25563-25568 Q9BZS1 denotes FOXP3
T12056 25690-25695 P23771 denotes GATA3
T12057 25721-25726 P23771 denotes GATA3
T12058 25740-25745 Q9BZS1 denotes FOXP3
T12059 25859-25864 P23771 denotes GATA3
T12060 25880-25885 Q9BZS1 denotes FOXP3
T12061 25909-25914 Q9BZS1 denotes FOXP3
T14975 27656-27661 Q9BZS1 denotes FOXP3
T14976 27728-27733 P23771 denotes GATA3
T14977 27882-27887 Q9BZS1 denotes FOXP3
T14978 27948-27951 P01730 denotes CD4
T14979 27952-27956 P01589 denotes CD25
T14980 27969-27973 P00519 denotes able
T14981 27989-27994 Q9BZS1 denotes FOXP3
T14982 28100-28105 Q9BZS1 denotes FOXP3
T14983 28258-28263 Q9BZS1 denotes FOXP3
T14984 28282-28287 P01137 denotes TGF-β
T14985 28338-28343 Q9BZS1 denotes FOXP3
T14986 28481-28486 Q9UL17 denotes T-bet
T14987 28491-28496 P23771 denotes GATA3
T14988 28625-28630 Q9BZS1 denotes FOXP3
T14989 28783-28788 P01137 denotes TGF-β
T14990 28835-28840 Q9BZS1 denotes FOXP3
T14991 28880-28885 P23771 denotes GATA3
T14992 28890-28895 Q9UL17 denotes T-bet
T14993 29005-29009 P05112 denotes IL-4
T14994 29011-29016 P29459 denotes IL-12
T14995 29011-29016 P29460 denotes IL-12
T14996 29021-29026 P01137 denotes TGF-β
T14997 29051-29056 Q9BZS1 denotes FOXP3
T14998 29107-29112 Q9BZS1 denotes FOXP3
T14999 29135-29140 P23771 denotes GATA3
T15000 29145-29150 Q9UL17 denotes T-bet
T15001 29243-29248 Q9BZS1 denotes FOXP3
T15002 29361-29366 P23771 denotes GATA3
T15003 29397-29402 P23771 denotes GATA3
T15004 29469-29474 Q9BZS1 denotes FOXP3
T15005 29536-29540 P05112 denotes IL-4
T15006 29549-29554 P35225 denotes IL-13
T15007 29578-29582 P00519 denotes able
T15008 29594-29599 P01137 denotes TGF-β
T15009 29609-29614 Q9BZS1 denotes FOXP3
T15010 29725-29729 P05112 denotes IL-4
T15011 29800-29804 P05112 denotes IL-4
T15012 29814-29818 P05112 denotes IL-4
T15013 29902-29906 P05112 denotes IL-4
T15014 29907-29911 P05112 denotes IL-4
T15015 29991-29995 P01589 denotes CD25
T15016 30001-30006 Q9BZS1 denotes Foxp3
T15017 30061-30065 P05112 denotes IL-4
T15018 30267-30271 P01589 denotes CD25
T15019 30313-30317 P05112 denotes IL-4
T15020 30335-30340 Q9BZS1 denotes Foxp3
T15021 30441-30446 Q9BZS1 denotes Foxp3
T15022 30459-30463 P05112 denotes IL-4
T15023 30559-30564 Q16552 denotes IL-17
T15024 30648-30652 P05231 denotes IL-6
T15025 30675-30680 P01137 denotes TGF-β
T15026 30891-30895 P05112 denotes IL-4
T15027 30900-30904 P05231 denotes IL-6
T15028 30906-30910 P05112 denotes IL-4
T15029 30965-30970 Q9BZS1 denotes FOXP3
T15030 30990-30993 P01730 denotes CD4
T15031 30994-30998 P01589 denotes CD25
T15032 31050-31054 P05112 denotes IL-4
T15033 31095-31099 P05112 denotes IL-4
T15034 31139-31144 Q9BZS1 denotes FOXP3
T15035 31221-31226 P23771 denotes GATA3
T15036 31239-31243 P05112 denotes IL-4
T15037 31309-31314 Q9BZS1 denotes FOXP3
T15038 31366-31371 P35225 denotes IL-13
T15039 31400-31405 Q9BZS1 denotes FOXP3
T15040 31426-31431 P23771 denotes GATA3
T15041 31458-31462 P05112 denotes IL-4
T15042 31487-31492 Q9BZS1 denotes FOXP3
T15043 31514-31519 P23771 denotes GATA3
T15044 31530-31535 P23771 denotes GATA3
T15045 31545-31549 P05112 denotes IL-4
T15046 31575-31580 P01137 denotes TGF-β
T15047 31590-31595 Q9BZS1 denotes FOXP3
T15048 31616-31620 P05112 denotes IL-4
T15049 31632-31637 P01137 denotes TGF-β
T15050 31715-31720 P23771 denotes GATA3
T15051 31725-31730 Q9BZS1 denotes FOXP3
T15052 31874-31879 P23771 denotes GATA3
T15053 31926-31931 Q9BZS1 denotes FOXP3
T15054 31957-31962 P23771 denotes GATA3
T15055 31992-31997 Q9BZS1 denotes FOXP3
T15056 32076-32081 P23771 denotes GATA3
T15057 32142-32147 P23771 denotes GATA3
T15058 32168-32171 P06729 denotes CD2
T15059 32172-32177 P23771 denotes GATA3
T15060 32241-32246 P23771 denotes GATA3
T15061 32302-32307 Q9BZS1 denotes FOXP3
T15062 32365-32370 P01137 denotes TGF-β
T15063 32396-32399 P06729 denotes CD2
T15064 32400-32405 P23771 denotes GATA3
T15065 32430-32435 Q9BZS1 denotes FOXP3
T15066 32535-32540 P23771 denotes GATA3
T15067 32775-32780 P23771 denotes GATA3
T15068 32874-32879 P23771 denotes GATA3
T15069 32903-32908 P23771 denotes GATA3
T15070 32995-33000 P23771 denotes GATA3
T15071 33045-33048 P01732 denotes CD8
T15072 33045-33048 P10966 denotes CD8
T15073 33196-33201 P23771 denotes GATA3
T15074 33287-33292 P23771 denotes GATA3
T15075 33303-33308 Q9BZS1 denotes FOXP3
T15076 33347-33352 Q9BZS1 denotes FOXP3
T15077 33505-33510 Q9BZS1 denotes FOXP3
T15078 33714-33719 P23771 denotes GATA3
T15079 33791-33796 P23771 denotes GATA3
T15080 33840-33845 P23771 denotes GATA3
T15081 33930-33935 P23771 denotes GATA3
T15082 33994-33999 P23771 denotes GATA3
T15083 34203-34208 P23771 denotes GATA3
T15084 34262-34267 Q9BZS1 denotes FOXP3
T15085 34328-34333 P23771 denotes GATA3
T15086 34345-34350 Q9BZS1 denotes FOXP3
T15087 34462-34466 P05412 denotes AP-1
T15088 34490-34495 P23771 denotes GATA3
T15089 34528-34532 P05412 denotes AP-1
T15090 34615-34620 Q9BZS1 denotes FOXP3
T15091 34666-34670 P05112 denotes IL-4
T18887 37820-37823 P01730 denotes CD4
T18888 37834-37837 P01730 denotes CD4
T18889 37840-37845 P14151 denotes CD62L
T18890 37852-37856 P01589 denotes CD25
T18891 38221-38226 Q9BZS1 denotes FOXP3
T19228 38323-38326 P01730 denotes CD4
T19229 38328-38334 P08575 denotes CD45RA
T19230 38357-38363 P08575 denotes CD45RO
T19231 38484-38487 P07766 denotes CD3
T19232 38484-38487 P20963 denotes CD3
T19233 38484-38487 P09693 denotes CD3
T19234 38484-38487 P04234 denotes CD3
T19235 38519-38523 P10747 denotes CD28
T19236 38562-38567 P29460 denotes IL-12
T19237 38562-38567 P29459 denotes IL-12
T19238 38582-38586 P05112 denotes IL-4
T19239 38630-38634 P05112 denotes IL-4
T19240 38649-38654 P01579 denotes IFN-γ
T19241 38669-38674 P29460 denotes IL-12
T19242 38669-38674 P29459 denotes IL-12
T19243 38722-38727 P01137 denotes TGF-β
T19244 38742-38747 P01579 denotes IFN-γ
T19245 38762-38767 P29459 denotes IL-12
T19246 38762-38767 P29460 denotes IL-12
T19247 38782-38786 P05112 denotes IL-4
T19248 38843-38847 P60568 denotes IL-2
T19874 39409-39412 P26358 denotes AIM
T19875 39467-39472 Q9BZS1 denotes FOXP3
T19876 39482-39492 P08659 denotes luciferase
T19877 39550-39553 P01730 denotes CD4
T19878 39779-39789 P08659 denotes luciferase
T19879 39824-39834 P08659 denotes luciferase
T19880 39960-39970 P08659 denotes luciferase
T20254 40294-40297 P04605 denotes TAT
T20255 40294-40297 P04608 denotes TAT
T20256 40294-40297 P04610 denotes TAT
T20257 40294-40297 Q8UMQ1 denotes TAT
T20258 40327-40332 P23771 denotes GATA3
T20259 40358-40363 P23771 denotes GATA3
T20260 40457-40460 P04610 denotes TAT
T20261 40457-40460 Q8UMQ1 denotes TAT
T20262 40457-40460 P04608 denotes TAT
T20263 40457-40460 P04605 denotes TAT
T20264 40631-40634 Q8UMQ1 denotes TAT
T20265 40631-40634 P04610 denotes TAT
T20266 40631-40634 P04608 denotes TAT
T20267 40631-40634 P04605 denotes TAT
T21956 43267-43272 Q9BZS1 denotes FOXP3
T21957 43312-43315 P01730 denotes CD4
T21958 43316-43320 P01589 denotes CD25
T21959 43651-43656 Q9BZS1 denotes FOXP3
T21960 43941-43946 P23771 denotes GATA3
T21961 43988-43993 Q9UL17 denotes T-bet
T21962 44033-44038 P04406 denotes GAPDH
T21963 44067-44072 Q15796 denotes SMAD2
T21964 44095-44100 P42226 denotes STAT6
T21965 44117-44122 P42226 denotes STAT6
T22362 44335-44339 P05112 denotes IL-4
T22363 44409-44413 P05112 denotes IL-4
T22364 44521-44524 P20963 denotes CD3
T22365 44521-44524 P09693 denotes CD3
T22366 44521-44524 P04234 denotes CD3
T22367 44521-44524 P07766 denotes CD3
T22368 44526-44529 P01730 denotes CD4
T22369 44535-44539 P01589 denotes CD25
T22370 44567-44571 P05112 denotes IL-4
T22371 44636-44640 P05112 denotes IL-4
T23182 45716-45719 P01730 denotes CD4
T23183 45848-45850 P0A7Z4 denotes pH
T23184 46599-46604 P23771 denotes GATA3
T23569 7411-7416 Q9BZS1 denotes FOXP3
T23570 7483-7486 P04234 denotes CD3
T23571 7483-7486 P20963 denotes CD3
T23572 7483-7486 P09693 denotes CD3
T23573 7483-7486 P07766 denotes CD3
T23574 7487-7491 P10747 denotes CD28
T23575 7508-7513 P01137 denotes TGF-β
T23576 7587-7592 Q9BZS1 denotes FOXP3
T23577 7763-7766 P09693 denotes CD3
T23578 7763-7766 P20963 denotes CD3
T23579 7763-7766 P07766 denotes CD3
T23580 7763-7766 P04234 denotes CD3
T23581 7767-7771 P10747 denotes CD28
T23582 7773-7778 P01137 denotes TGF-β
T23583 7788-7792 P05112 denotes IL-4
T23897 10165-10170 Q9BZS1 denotes FOXP3
T23898 10206-10211 Q9BZS1 denotes FOXP3
T23899 10342-10347 P01137 denotes TGF-β
T23900 10349-10354 Q9BZS1 denotes FOXP3
T24279 10632-10636 P05112 denotes IL-4
T24280 10658-10663 Q9BZS1 denotes FOXP3
T24281 10699-10704 Q9BZS1 denotes FOXP3
T24282 10709-10713 P05112 denotes IL-4
T24283 10762-10765 P01730 denotes CD4
T24284 10802-10808 P08575 denotes CD45RO
T24285 10813-10817 P01589 denotes CD25
T24286 10892-10898 P08575 denotes CD45RO
T24287 10899-10903 P01589 denotes CD25
T24288 10926-10932 P08575 denotes CD45RO
T24289 10933-10937 P01589 denotes CD25
T24290 10962-10968 P08575 denotes CD45RO
T24291 10969-10973 P01589 denotes CD25
T24292 11261-11265 P05112 denotes IL-4
T24293 11303-11309 P08575 denotes CD45RO
T24294 11328-11333 Q9BZS1 denotes FOXP3
T24295 11338-11342 P05112 denotes IL-4
T24803 13213-13218 Q9BZS1 denotes FOXP3
T24804 13274-13277 P01730 denotes CD4
T24805 13278-13284 P08575 denotes CD45RA
T24806 13331-13334 P09693 denotes CD3
T24807 13331-13334 P20963 denotes CD3
T24808 13331-13334 P07766 denotes CD3
T24809 13331-13334 P04234 denotes CD3
T24810 13335-13339 P10747 denotes CD28
T24811 13359-13364 P01137 denotes TGF-β
T24812 13378-13382 P05112 denotes IL-4
T24813 13491-13496 Q9BZS1 denotes FOXP3
T24814 13501-13506 P23771 denotes GATA3
T24815 13596-13601 P23771 denotes GATA3
T24816 13606-13611 Q9BZS1 denotes FOXP3
T24817 13648-13651 P01730 denotes CD4
T24818 13652-13658 P08575 denotes CD45RA
T25354 16508-16512 P05112 denotes IL-4
T25355 16516-16521 Q9BZS1 denotes FOXP3
T25356 16599-16604 P01137 denotes TGF-β
T25357 16636-16640 P05112 denotes IL-4
T25358 16869-16872 P01730 denotes CD4
T25359 16873-16879 P08575 denotes CD45RA
T25360 16949-16954 P01137 denotes TGF-β
T25361 17001-17005 P05112 denotes IL-4
T25362 17081-17084 P01730 denotes CD4
T25363 17085-17091 P08575 denotes CD45RA
T25364 17146-17149 P20963 denotes CD3
T25365 17146-17149 P07766 denotes CD3
T25366 17146-17149 P04234 denotes CD3
T25367 17146-17149 P09693 denotes CD3
T25368 17150-17154 P10747 denotes CD28
T25369 17156-17161 P01137 denotes TGF-β
T25370 17177-17181 P05112 denotes IL-4
T25371 17294-17299 Q15796 denotes SMAD2
T25372 17304-17309 P42226 denotes STAT6
T25373 17317-17322 P42226 denotes STAT6
T25374 17327-17332 P04406 denotes GAPDH
T25375 17379-17384 P23771 denotes GATA3
T25376 17389-17394 Q9BZS1 denotes FOXP3
T25377 17432-17435 P01730 denotes CD4
T25378 17436-17442 P08575 denotes CD45RA
T25379 17455-17459 P05112 denotes IL-4
T25986 18665-18669 P05112 denotes IL-4
T25987 18679-18684 P01137 denotes TGF-β
T25988 18724-18727 P01730 denotes CD4
T25989 18728-18734 P08575 denotes CD45RA
T25990 18779-18782 P09693 denotes CD3
T25991 18779-18782 P20963 denotes CD3
T25992 18779-18782 P07766 denotes CD3
T25993 18779-18782 P04234 denotes CD3
T25994 18783-18787 P10747 denotes CD28
T25995 18789-18794 P01137 denotes TGF-β
T25996 18800-18804 P05112 denotes IL-4
T25997 19101-19106 P23771 denotes GATA3
T25998 19111-19116 Q9BZS1 denotes FOXP3
T25999 19168-19171 P01730 denotes CD4
T26000 19172-19178 P08575 denotes CD45RA
T26001 19196-19199 P07766 denotes CD3
T26002 19196-19199 P20963 denotes CD3
T26003 19196-19199 P04234 denotes CD3
T26004 19196-19199 P09693 denotes CD3
T26005 19204-19208 P05112 denotes IL-4
T26006 19213-19218 P01137 denotes TGF-β
T26495 22450-22455 P23771 denotes GATA3
T26496 22488-22493 Q9BZS1 denotes FOXP3
T26497 22521-22524 P01730 denotes CD4
T26498 22586-22589 P04608 denotes TAT
T26499 22586-22589 P04610 denotes TAT
T26500 22586-22589 P04605 denotes TAT
T26501 22586-22589 Q8UMQ1 denotes TAT
T26502 22590-22595 P23771 denotes GATA3
T26503 22635-22640 P23771 denotes GATA3
T26504 22680-22683 P04234 denotes CD3
T26505 22680-22683 P07766 denotes CD3
T26506 22680-22683 P20963 denotes CD3
T26507 22680-22683 P09693 denotes CD3
T26508 22684-22688 P10747 denotes CD28
T26509 22714-22717 Q9U6Y4 denotes GFP
T26510 22775-22780 Q9BZS1 denotes FOXP3
T26511 22902-22905 P01730 denotes CD4
T26512 22906-22910 P01589 denotes CD25
T26513 22963-22968 P23771 denotes GATA3
T26514 22991-22994 E2RVI8 denotes OVA
T26515 22999-23004 P01137 denotes TGF-β
T26516 23023-23026 P01730 denotes CD4
T26517 23045-23050 Q9BZS1 denotes FOXP3
T26518 23240-23245 P01137 denotes TGF-β
T27223 25937-25942 P23771 denotes GATA3
T27224 25963-25968 Q9BZS1 denotes FOXP3
T27225 26019-26022 P01730 denotes CD4
T27226 26116-26121 Q9BZS1 denotes FOXP3
T27227 26151-26161 P08659 denotes luciferase
T27228 26249-26259 P08659 denotes luciferase
T27229 26341-26344 P01730 denotes CD4
T27230 26379-26384 Q9BZS1 denotes FOXP3
T27231 26429-26434 P23771 denotes GATA3
T27232 26578-26581 P01730 denotes CD4
T27233 26627-26632 P23771 denotes GATA3
T27234 26645-26650 Q9BZS1 denotes FOXP3
T27235 26789-26799 P08659 denotes luciferase
T27236 26939-26944 P23771 denotes GATA3
T27237 27165-27170 P23771 denotes GATA3
T27238 27241-27246 P23771 denotes GATA3
T27239 27407-27412 P23771 denotes GATA3
T27240 27428-27433 Q9BZS1 denotes FOXP3
T6960 13785-13789 P05112 denotes IL-4
T6961 13799-13804 P01137 denotes TGF-β
T6962 13831-13835 P05112 denotes IL-4
T6963 13956-13960 P05112 denotes IL-4
T6964 13964-13968 P00519 denotes able
T6965 13980-13985 P01137 denotes TGF-β
T6966 13999-14004 Q9BZS1 denotes FOXP3
T6967 14048-14051 P01730 denotes CD4
T6968 14052-14058 P08575 denotes CD45RA
T17881 35397-35403 P23771 denotes GATA-3
T17882 35425-35428 P06729 denotes CD2
T17883 35451-35454 P06729 denotes CD2
T17884 35455-35460 P23771 denotes GATA3
T17885 35493-35498 P23771 denotes GATA3
T18343 36389-36394 Q9BZS1 denotes FOXP3
T18344 36448-36453 Q9BZS1 denotes FOXP3
T18345 36558-36563 P23771 denotes GATA3
T18346 37097-37100 P04608 denotes TAT
T18347 37097-37100 P04610 denotes TAT
T18348 37097-37100 Q8UMQ1 denotes TAT
T18349 37097-37100 P04605 denotes TAT
T18350 37115-37118 P04591 denotes GAG
T18351 37216-37219 P21980 denotes TGC
T18352 37666-37674 Q04864 denotes Relative
T21245 41610-41615 Q9BZS1 denotes FOXP3
T21246 41707-41711 P01589 denotes CD25
T21247 41851-41856 Q9BZS1 denotes FOXP3
T21248 42220-42225 P14151 denotes CD62L
T21249 42483-42486 P09693 denotes CD3
T21250 42483-42486 P07766 denotes CD3
T21251 42483-42486 P04234 denotes CD3
T21252 42483-42486 P20963 denotes CD3
T21253 42493-42496 P01730 denotes CD4
T21254 42507-42511 P01589 denotes CD25
T21255 42646-42649 P01730 denotes CD4
T21256 42693-42698 P23771 denotes GATA3
T21257 42737-42741 P01589 denotes CD25
T21258 42753-42758 P14151 denotes CD62L
T21259 42772-42775 P01730 denotes CD4
T21260 42885-42890 Q9BZS1 denotes Foxp3
T21261 42975-42980 Q9BZS1 denotes Foxp3
T21262 43050-43053 P01730 denotes CD4
T15092 34672-34677 P23771 denotes GATA3
T15093 34702-34707 Q9BZS1 denotes FOXP3
T15094 34830-34835 Q9BZS1 denotes FOXP3
T15095 34891-34895 P05112 denotes Il-4
T15096 34937-34942 Q9BZS1 denotes FOXP3
T18156 35795-35798 P01730 denotes CD4
T18157 35809-35812 P01730 denotes CD4
T18158 35890-35893 P01730 denotes CD4
T18159 35979-35982 P01730 denotes CD4
T18160 36182-36186 P05112 denotes IL-4
T19605 38876-38881 Q9BZS1 denotes FOXP3
T19606 38934-38939 Q9BZS1 denotes FOXP3
T19607 39026-39031 Q9BZS1 denotes FOXP3
T19608 39081-39086 Q9BZS1 denotes FOXP3
T19609 39278-39281 P04591 denotes GAG
T19610 39286-39289 Q8UMQ1 denotes TAT
T19611 39286-39289 P04610 denotes TAT
T19612 39286-39289 P04608 denotes TAT
T19613 39286-39289 P04605 denotes TAT
T20520 40747-40750 Q8UMQ1 denotes TAT
T20521 40747-40750 P04608 denotes TAT
T20522 40747-40750 P04610 denotes TAT
T20523 40747-40750 P04605 denotes TAT
T20524 40751-40756 P23771 denotes GATA3
T20525 40771-40774 P01730 denotes CD4
T20526 40775-40781 P08575 denotes CD45RA
T20527 40890-40895 P23771 denotes GATA3
T20528 40985-40988 P07766 denotes CD3
T20529 40985-40988 P04234 denotes CD3
T20530 40985-40988 P09693 denotes CD3
T20531 40985-40988 P20963 denotes CD3
T20532 40998-41002 P10747 denotes CD28
T20533 41007-41012 P01137 denotes TGF-β
T20534 41039-41042 Q8UMQ1 denotes TAT
T20535 41039-41042 P04610 denotes TAT
T20536 41039-41042 P04608 denotes TAT
T20537 41039-41042 P04605 denotes TAT
T20538 41086-41091 Q9BZS1 denotes FOXP3
T20788 41316-41320 P05112 denotes IL-4
T22743 45111-45116 P23771 denotes GATA3
T22744 45490-45495 Q9BZS1 denotes FOXP3

test2

Id Subject Object Predicate Lexical cue Negation Speculation
T31 0-5 Protein denotes GATA3
T32 27-34 Negative_regulation denotes Inhibit
T33 35-41 Protein denotes TGF-β1
T34 42-49 Positive_regulation denotes Induced true
T35 50-55 Protein denotes FOXP3
T36 56-66 Gene_expression denotes Expression
T37 388-393 Protein denotes FOXP3
T38 708-721 Protein denotes interleukin 4
T39 723-727 Protein denotes IL-4
T40 767-775 Negative_regulation denotes inhibits
T41 776-781 Protein denotes FOXP3
T42 875-889 Gene_expression denotes overexpressing
T43 890-896 Protein denotes GATA-3
T44 931-940 Negative_regulation denotes deficient
T45 944-978 Protein denotes transforming growth factor (TGF)-β
T46 979-987 Positive_regulation denotes mediated
T47 988-993 Protein denotes FOXP3
T48 994-1003 Positive_regulation denotes induction
T49 1043-1050 Binding denotes binding
T50 1054-1059 Protein denotes GATA3
T51 1067-1072 Protein denotes FOXP3
T52 1073-1081 Entity denotes promoter
T53 1245-1249 Protein denotes IL-4
T54 1344-1350 Binding denotes target
T55 1351-1355 Protein denotes IL-4
T56 1359-1364 Protein denotes GATA3
T867 3496-3509 Protein denotes interleukin 4
T868 3511-3515 Protein denotes IL-4
T869 3517-3524 Positive_regulation denotes induced
T870 3525-3530 Protein denotes STAT6
T871 3535-3540 Protein denotes GATA3
T872 3620-3625 Protein denotes GATA3
T873 3700-3709 Positive_regulation denotes induction
T874 3713-3717 Protein denotes IL-4
T875 3719-3723 Protein denotes IL-5
T876 3728-3733 Protein denotes IL-13
T877 3848-3853 Protein denotes T-bet
T878 3863-3872 Positive_regulation denotes modulates
T879 3873-3878 Protein denotes GATA3
T880 4077-4082 Protein denotes GATA3
T881 4177-4181 Protein denotes IL-4
T882 4251-4265 Positive_regulation denotes Overexpression
T883 4271-4279 Negative_regulation denotes dominant
T884 4297-4302 Protein denotes GATA3
T885 4335-4343 Positive_regulation denotes mediated
T886 4344-4349 Protein denotes GATA3
T887 4350-4358 Negative_regulation denotes blockade
T888 4756-4763 Gene_expression denotes express
T889 4798-4803 Protein denotes FOXP3
T890 4823-4830 Gene_expression denotes express
T891 4831-4835 Protein denotes CD25
T892 5098-5105 Gene_expression denotes express
T893 5106-5111 Protein denotes FOXP3
T894 5227-5233 Positive_regulation denotes induce
T895 5234-5239 Protein denotes FOXP3
T896 5240-5250 Gene_expression denotes expression
T897 5356-5361 Protein denotes TGF-β
T898 5480-5487 Negative_regulation denotes lacking
T899 5492-5500 Protein denotes TGF-βRII
T900 5649-5654 Protein denotes TGF-β
T901 5833-5838 Protein denotes GATA3
T902 5843-5848 Protein denotes FOXP3
T903 5901-5906 Protein denotes T-bet
T904 5911-5916 Protein denotes GATA3
T905 5977-5982 Protein denotes GATA3
T906 5983-5991 Negative_regulation denotes inhibits
T907 5992-5997 Protein denotes FOXP3
T908 5998-6007 Positive_regulation denotes induction
T909 6017-6021 Protein denotes IL-4
T910 6029-6034 Protein denotes FOXP3
T911 6035-6045 Gene_expression denotes expression
T912 6051-6056 Protein denotes GATA3
T913 6057-6065 Positive_regulation denotes mediated
T914 6116-6121 Protein denotes GATA3
T915 6131-6136 Binding denotes binds
T916 6144-6149 Protein denotes FOXP3
T917 6150-6158 Entity denotes promoter
T2744 6284-6289 Protein denotes FOXP3
T2745 6290-6299 Positive_regulation denotes Induction
T2746 6337-6342 Protein denotes FOXP3
T2747 6343-6353 Gene_expression denotes expression
T2748 6361-6368 Positive_regulation denotes induced true
T2749 6485-6488 Protein denotes CD4
T2750 6524-6531 Gene_expression denotes express true
T2751 6532-6537 Protein denotes FOXP3 true
T2752 6562-6567 Protein denotes FOXP3
T2753 6575-6584 Gene_expression denotes expressed true
T2754 6612-6622 Gene_expression denotes expression
T2755 6660-6665 Protein denotes FOXP3
T2756 6666-6681 Transcription denotes mRNA expression
T2757 6731-6735 Protein denotes CD25 true
T2758 6745-6748 Protein denotes CD4
T2759 6757-6763 Protein denotes CD45RA
T2760 6774-6780 Protein denotes CD45RO true
T2761 6923-6926 Protein denotes CD4
T2762 6927-6931 Protein denotes CD25
T2763 6934-6940 Protein denotes CD45RA
T2764 6943-6949 Protein denotes CD45RO
T2765 6956-6959 Protein denotes CD4
T2766 6960-6966 Protein denotes CD45RO
T2767 6967-6971 Protein denotes CD25
T2768 7000-7006 Positive_regulation denotes induce
T2769 7007-7012 Protein denotes FOXP3 true
T2770 7018-7023 Positive_regulation denotes up to
T2771 7068-7073 Protein denotes TGF-β true
T2772 7185-7193 Positive_regulation denotes increase
T2773 7194-7199 Protein denotes FOXP3
T2774 7200-7210 Gene_expression denotes expression
T2775 7270-7281 Positive_regulation denotes up-regulate
T2776 7282-7287 Protein denotes FOXP3
T2777 7980-7987 Gene_expression denotes produce
T2778 7988-7992 Protein denotes IL-4
T2779 7998-8008 Positive_regulation denotes activation
T2780 8020-8028 Binding denotes interact
T2781 8034-8039 Protein denotes TGF-β true
T2782 8059-8066 Negative_regulation denotes prevent true
T2783 8067-8072 Protein denotes FOXP3
T2784 8073-8082 Positive_regulation denotes induction true
T2785 8097-8111 Negative_regulation denotes neutralization
T2786 8115-8119 Protein denotes IL-4
T2787 8127-8135 Negative_regulation denotes blocking
T2788 8136-8140 Protein denotes IL-4
T2789 8158-8164 Positive_regulation denotes rescue
T2790 8165-8170 Protein denotes FOXP3 true
T2791 8171-8181 Gene_expression denotes expression true
T2792 8300-8307 Gene_expression denotes express
T2793 8308-8313 Protein denotes FOXP3
T2794 8357-8364 Gene_expression denotes express
T2795 8365-8370 Protein denotes FOXP3
T2796 8443-8447 Negative_regulation denotes lack
T2797 8448-8453 Protein denotes FOXP3
T2798 8454-8464 Gene_expression denotes expression
T2799 8495-8504 Gene_expression denotes expressed
T2800 8505-8510 Protein denotes FOXP3
T2801 8586-8593 Gene_expression denotes express
T2802 8594-8599 Protein denotes FOXP3
T2803 8609-8612 Positive_regulation denotes low
T2804 8613-8617 Protein denotes CD25
T2805 8626-8636 Gene_expression denotes expression
T2806 8659-8664 Protein denotes TGF-β
T2807 8681-8689 Positive_regulation denotes increase
T2808 8694-8699 Protein denotes FOXP3
T2809 8700-8710 Gene_expression denotes expression
T2810 8748-8755 Positive_regulation denotes induced
T2811 8756-8761 Protein denotes FOXP3
T2812 8762-8772 Gene_expression denotes expression
T2813 8825-8830 Protein denotes CRTH2
T2814 8877-8881 Protein denotes IL-4
T2815 8882-8891 Gene_expression denotes secretion
T2816 8899-8903 Protein denotes IL-4
T2817 8904-8913 Gene_expression denotes producing
T2818 8943-8950 Negative_regulation denotes lacking
T2819 8951-8956 Protein denotes FOXP3
T2820 8957-8967 Gene_expression denotes expression
T2821 8993-8998 Protein denotes TGF-β
T2822 8999-9007 Positive_regulation denotes mediated
T2823 9008-9013 Protein denotes FOXP3
T2824 9014-9023 Positive_regulation denotes induction
T2825 9024-9030 Negative_regulation denotes failed
T2826 9100-9104 Protein denotes IL-4
T2827 9134-9144 Gene_expression denotes expression
T2828 9148-9152 Protein denotes IL-4
T2829 9157-9162 Protein denotes FOXP3
T2830 9183-9186 Protein denotes CD4
T2831 9256-9260 Protein denotes IL-4
T2832 9261-9271 Gene_expression denotes expressing
T2833 9303-9309 Protein denotes CD45RO
T2834 9310-9314 Protein denotes CD25
T2835 9337-9340 Gene_expression denotes co-
T2836 9340-9347 Gene_expression denotes express
T2837 9348-9353 Protein denotes FOXP3
T2838 9392-9398 Protein denotes CD45RO
T2839 9399-9403 Protein denotes CD25
T2840 9422-9431 Gene_expression denotes expressed
T2841 9432-9437 Protein denotes FOXP3
T2842 9445-9452 Negative_regulation denotes lacking
T2843 9453-9457 Protein denotes IL-4
T2844 9528-9532 Protein denotes IL-4
T2845 9567-9572 Protein denotes FOXP3
T2846 9609-9613 Protein denotes IL-4
T2847 9642-9647 Protein denotes FOXP3
T2848 9673-9679 Protein denotes CD45RO
T2849 9680-9684 Protein denotes CD25
T2850 9687-9693 Protein denotes CD45RO
T2851 9694-9698 Protein denotes CD25
T2852 9705-9711 Protein denotes CD45RO
T2853 9712-9716 Protein denotes CD25
T2854 9786-9791 Protein denotes CRTH2
T2855 9812-9821 Gene_expression denotes expressed
T2856 9822-9827 Protein denotes FOXP3
T2857 9866-9870 Protein denotes IL-4
T2858 9871-9880 Localization denotes secretion
T2859 9941-9946 Protein denotes FOXP3
T2860 9947-9957 Gene_expression denotes expressing
T2861 9986-9996 Gene_expression denotes expression
T2862 10055-10060 Protein denotes FOXP3
T2863 10069-10078 Gene_expression denotes expressed
T2864 10104-10113 Positive_regulation denotes inducible
T5120 11403-11408 Protein denotes FOXP3
T5121 11413-11418 Protein denotes GATA3
T5122 11510-11516 Positive_regulation denotes induce true
T5123 11517-11522 Protein denotes FOXP3 true
T5124 11523-11533 Gene_expression denotes expression
T5125 11554-11563 Positive_regulation denotes induction
T5126 11651-11661 Gene_expression denotes expression
T5127 11665-11670 Protein denotes FOXP3
T5128 11675-11680 Protein denotes GATA3
T5129 11717-11720 Protein denotes CD4
T5130 11753-11757 Protein denotes IL-4
T5131 11764-11769 Protein denotes IFN-γ
T5132 11780-11785 Protein denotes IL-12
T5133 11864-11869 Protein denotes FOXP3 true
T5134 11874-11879 Protein denotes GATA3
T5135 12039-12044 Protein denotes FOXP3
T5136 12045-12060 Transcription denotes mRNA expression
T5137 12061-12070 Positive_regulation denotes increased
T5138 12127-12132 Protein denotes GATA3
T5139 12137-12142 Protein denotes FOXP3
T5140 12174-12184 Gene_expression denotes expression
T5141 12357-12367 Gene_expression denotes expressing
T5142 12373-12378 Protein denotes FOXP3
T5143 12403-12412 Gene_expression denotes expressed
T5144 12427-12432 Protein denotes GATA3
T5145 12455-12465 Gene_expression denotes expression
T5146 12489-12504 Transcription denotes mRNA expression
T5147 12703-12706 Protein denotes CD3
T5148 12736-12741 Protein denotes CD103
T5149 12743-12749 Protein denotes CTLA-4
T5150 12751-12755 Protein denotes GITR
T5151 12761-12765 Protein denotes PD-1
T5152 12774-12784 Gene_expression denotes expression
T5153 12894-12899 Protein denotes GATA3
T5154 12904-12909 Protein denotes FOXP3
T5155 12910-12920 Gene_expression denotes expression
T5156 13079-13083 Negative_regulation denotes lost
T5157 13102-13109 Gene_expression denotes express
T5158 13110-13115 Protein denotes FOXP3
T6292 13785-13789 Protein denotes IL-4 true
T6293 13799-13804 Protein denotes TGF-β
T6294 13831-13835 Protein denotes IL-4
T6295 13956-13960 Protein denotes IL-4
T6296 13972-13979 Negative_regulation denotes inhibit
T6297 13980-13985 Protein denotes TGF-β true
T6298 13986-13995 Positive_regulation denotes induction
T6299 13999-14004 Protein denotes FOXP3
T6300 14048-14051 Protein denotes CD4
T6301 14052-14058 Protein denotes CD45RA
T6302 14105-14108 Protein denotes CD3
T6303 14109-14113 Protein denotes CD28
T6304 14133-14138 Protein denotes TGF-β
T6305 14146-14150 Protein denotes IL-4 true
T6306 14176-14180 Protein denotes IL-4
T6307 14193-14202 Negative_regulation denotes repressed
T6308 14207-14212 Protein denotes TGF-β true
T6309 14222-14231 Positive_regulation denotes induction true
T6310 14235-14240 Protein denotes FOXP3 true
T6311 14241-14251 Gene_expression denotes expression true
T6312 14327-14332 Protein denotes GATA3
T6313 14338-14345 Positive_regulation denotes induced
T6314 14358-14365 Negative_regulation denotes absence
T6315 14369-14373 Protein denotes IL-4
T6316 14441-14445 Protein denotes IL-4
T6317 14469-14475 Positive_regulation denotes induce true
T6318 14476-14481 Protein denotes FOXP3 true
T6319 14482-14492 Gene_expression denotes expression
T6320 14503-14508 Protein denotes GATA3 true
T6321 14518-14525 Positive_regulation denotes induced true
T6322 14545-14550 Protein denotes TGF-β
T6323 14554-14558 Positive_regulation denotes high
T6324 14559-14563 Protein denotes IL-4
T6325 14602-14606 Protein denotes IL-4
T6326 14616-14626 Positive_regulation denotes prevention
T6327 14630-14635 Protein denotes FOXP3
T6328 14636-14646 Gene_expression denotes expression
T6329 14655-14661 Positive_regulation denotes caused
T6330 14718-14733 Phosphorylation denotes phosphorylation
T6331 14737-14742 Protein denotes SMAD2
T6332 14746-14751 Protein denotes STAT6
T6333 14760-14768 Regulation denotes affected
T6334 14788-14792 Protein denotes IL-4
T6335 14800-14805 Protein denotes TGF-β
T6336 14831-14835 Protein denotes IL-4
T6337 14847-14852 Protein denotes TGF-β
T6338 14937-14941 Protein denotes IL-4
T6339 14942-14950 Positive_regulation denotes increase
T6340 14965-14970 Protein denotes GATA3
T6341 14980-14985 Protein denotes FOXP3
T6342 14986-14995 Negative_regulation denotes decreased
T6343 15079-15083 Protein denotes IL-4
T6344 15258-15265 Gene_expression denotes express
T6345 15274-15278 Protein denotes CD25
T6346 15319-15323 Protein denotes IL-4
T6347 15325-15331 Protein denotes rmIL-4
T6348 15343-15347 Protein denotes IL-4
T6349 15500-15503 Protein denotes CD4
T6350 15504-15508 Protein denotes CD25
T6351 15514-15519 Protein denotes FOXP3
T6352 15646-15652 Protein denotes rmIL-4
T6353 15663-15667 Protein denotes IL-4
T6354 15703-15706 Protein denotes CD4
T6355 15707-15711 Protein denotes CD25
T6356 15736-15741 Protein denotes Foxp3
T6357 15751-15761 Negative_regulation denotes diminished
T6358 15863-15866 Protein denotes CD4
T6359 15867-15871 Protein denotes CD25
T6360 15903-15906 Protein denotes CD4
T6361 15907-15911 Protein denotes CD25
T6362 15937-15941 Protein denotes IL-4
T6363 16056-16063 Regulation denotes effects
T6364 16067-16071 Protein denotes IL-4
T6365 16149-16153 Protein denotes CD25
T6366 16180-16184 Protein denotes IL-4
T6367 16198-16203 Protein denotes FOXP3
T6368 16204-16214 Gene_expression denotes expression
T6369 16273-16277 Protein denotes IL-4
T6370 16288-16295 Negative_regulation denotes inhibit
T6371 16296-16301 Protein denotes FOXP3
T6372 16302-16312 Gene_expression denotes expression
T6373 16429-16437 Negative_regulation denotes decrease
T6374 16438-16443 Protein denotes FOXP3
T6375 16444-16454 Gene_expression denotes expression
T6376 16460-16464 Protein denotes IL-4
T8197 17557-17562 Protein denotes TGF-β
T8198 17598-17603 Protein denotes TGF-β
T8199 17604-17611 Negative_regulation denotes reduced
T8200 17612-17616 Protein denotes CD25
T8201 17618-17622 Protein denotes IL-4
T8202 17623-17633 Gene_expression denotes expression
T8203 17639-17643 Protein denotes CD25
T8204 17644-17654 Gene_expression denotes expression
T8205 17668-17672 Protein denotes IL-4
T8206 17687-17696 Negative_regulation denotes inhibited
T8207 17697-17702 Protein denotes TGF-β true
T8208 17703-17711 Positive_regulation denotes mediated
T8209 17712-17721 Positive_regulation denotes induction
T8210 17725-17730 Protein denotes FOXP3
T8211 17762-17767 Protein denotes FOXP3
T8212 17834-17838 Protein denotes IL-4 true
T8213 17910-17915 Protein denotes FOXP3 true
T8214 17932-17940 Negative_regulation denotes decrease
T8215 17968-17973 Protein denotes FOXP3
T8216 18061-18065 Protein denotes IL-4
T8217 18112-18117 Protein denotes FOXP3
T8218 18132-18137 Protein denotes FOXP3
T8219 18234-18239 Protein denotes TGF-β
T8220 18249-18258 Positive_regulation denotes induction true
T8221 18262-18267 Protein denotes FOXP3
T8222 18268-18278 Gene_expression denotes expression
T8223 18287-18293 Positive_regulation denotes caused true
T8224 18313-18317 Protein denotes CD25 true
T8225 18318-18323 Protein denotes FOXP3
T8226 18355-18360 Protein denotes FOXP3
T8227 18399-18402 Protein denotes CD4
T8228 18403-18409 Protein denotes CD45RA
T8229 18448-18453 Protein denotes FOXP3
T8230 18508-18512 Protein denotes CD25
T8231 18517-18531 Negative_regulation denotes down-regulated
T8232 18535-18540 Protein denotes TGF-β
T8233 18639-18644 Protein denotes TGF-β
T8234 18649-18653 Protein denotes IL-4
T8235 19296-19300 Protein denotes IL-4
T8236 19353-19358 Protein denotes FOXP3
T8237 19419-19423 Protein denotes IL-4
T8238 19424-19433 Gene_expression denotes producing
T8239 19448-19453 Protein denotes FOXP3
T8240 19454-19464 Gene_expression denotes expressing
T8241 19509-19514 Protein denotes FOXP3
T8242 19597-19602 Protein denotes GATA3
T8243 19607-19612 Protein denotes FOXP3
T8244 19613-19623 Gene_expression denotes expression
T8245 19676-19680 Protein denotes IL-4
T8246 19685-19690 Protein denotes TGF-β
T8247 19709-19714 Protein denotes GATA3
T8248 19719-19724 Protein denotes FOXP3
T8249 19794-19804 Gene_expression denotes co-express
T8250 19950-19955 Protein denotes GATA3
T8251 19999-20009 Negative_regulation denotes repressing
T8252 20010-20015 Protein denotes FOXP3
T8253 20043-20047 Protein denotes IL-4
T8254 20053-20064 Positive_regulation denotes in vitro as
T8255 20065-20077 Negative_regulation denotes an inhibitor
T8256 20081-20086 Protein denotes FOXP3
T8257 20087-20097 Gene_expression denotes expression
T8258 20107-20118 Negative_regulation denotes interfering
T8259 20124-20129 Protein denotes TGF-β
T8260 20214-20219 Protein denotes GATA3
T9665 20242-20247 Protein denotes GATA3
T9666 20262-20271 Regulation denotes Regulator
T9667 20275-20280 Protein denotes FOXP3
T9668 20281-20291 Gene_expression denotes Expression
T9669 20292-20297 Protein denotes FOXP3
T9670 20298-20308 Gene_expression denotes expression
T9671 20309-20318 Negative_regulation denotes decreased true
T9672 20324-20329 Protein denotes GATA3
T9673 20330-20340 Gene_expression denotes expression
T9674 20389-20393 Regulation denotes role
T9675 20398-20403 Protein denotes GATA3 true
T9676 20407-20417 Negative_regulation denotes repressing
T9677 20418-20423 Protein denotes FOXP3
T9678 20433-20438 Protein denotes GATA3
T9679 20488-20493 Protein denotes GATA3
T9680 20572-20582 Negative_regulation denotes inhibiting
T9681 20583-20588 Protein denotes STAT4 true
T9682 20589-20599 Gene_expression denotes expression
T9683 20622-20627 Protein denotes GATA3 true
T9684 20692-20697 Protein denotes GATA3
T9685 20711-20718 Negative_regulation denotes inhibit true
T9686 20719-20724 Protein denotes FOXP3 true
T9687 20725-20734 Positive_regulation denotes induction
T9688 20739-20749 Positive_regulation denotes transduced
T9689 20750-20755 Protein denotes GATA3
T9690 20761-20770 Negative_regulation denotes truncated
T9691 20771-20776 Protein denotes GATA3
T9692 20777-20784 Negative_regulation denotes lacking
T9693 20789-20807 Entity denotes DNA-binding domain
T9694 20825-20828 Protein denotes CD4
T9695 20829-20835 Protein denotes CD45RA
T9696 20853-20893 Protein denotes TAT-fused, recombinantly expressed GATA3
T9697 20958-20961 Protein denotes CD3
T9698 20962-20966 Protein denotes CD28
T9699 20986-20993 Negative_regulation denotes absence
T9700 20997-21002 Protein denotes TGF-β
T9701 21004-21013 Protein denotes TAT-GATA3
T9702 21096-21099 Protein denotes CD4
T9703 21135-21144 Protein denotes TAT-GATA3
T9704 21145-21152 Negative_regulation denotes reduced
T9705 21153-21158 Protein denotes FOXP3
T9706 21159-21169 Gene_expression denotes expression
T9707 21180-21189 Regulation denotes dependent
T9708 21208-21226 Entity denotes DNA-binding domain
T9709 21255-21260 Protein denotes GATA3
T9710 21270-21276 Regulation denotes affect
T9711 21277-21282 Protein denotes FOXP3
T9712 21283-21293 Gene_expression denotes expression
T9713 21311-21321 Gene_expression denotes expression
T9714 21397-21403 Regulation denotes effect
T9715 21407-21412 Protein denotes GATA3
T9716 21416-21421 Protein denotes FOXP3
T9717 21465-21479 Positive_regulation denotes overexpressing
T9718 21480-21485 Protein denotes GATA3
T9719 21511-21514 Protein denotes CD2
T9720 21542-21548 Protein denotes 10xCD2
T9721 21549-21554 Protein denotes GATA3
T9722 21587-21590 Protein denotes CD4
T9723 21625-21631 Protein denotes 10xCD2
T9724 21632-21637 Protein denotes GATA3
T9725 21803-21808 Protein denotes GATA3
T9726 21819-21822 Protein denotes CD4
T9727 21823-21828 Protein denotes CD62L
T9728 21829-21833 Protein denotes CD25
T9729 21905-21910 Protein denotes TGF-β
T9730 21916-21921 Protein denotes Foxp3
T9731 21922-21932 Gene_expression denotes expression
T9732 21967-21970 Protein denotes CD4
T9733 21971-21975 Protein denotes CD25
T9734 21988-21993 Protein denotes Foxp3
T9735 22049-22054 Protein denotes TGF-β
T9736 22068-22080 Positive_regulation denotes up-regulated
T9737 22081-22086 Protein denotes Foxp3
T9738 22155-22158 Protein denotes CD2
T9739 22159-22169 Protein denotes GATA3xDO11
T9740 22198-22205 Negative_regulation denotes reduced
T9741 22206-22211 Protein denotes Foxp3
T9742 22212-22222 Gene_expression denotes expression
T9743 22228-22237 Positive_regulation denotes activated
T9744 22243-22248 Protein denotes TGF-β
T9745 22279-22287 Gene_expression denotes produced
T9746 22307-22312 Protein denotes TGF-β
T9747 22327-22332 Protein denotes Smad7
T9748 22345-22354 Gene_expression denotes expressed
T9749 22423-22428 Protein denotes TGF-β
T9750 23382-23386 Protein denotes IL-4
T9751 23387-23394 Positive_regulation denotes induced
T9752 23395-23400 Protein denotes GATA3
T11427 23457-23462 Protein denotes GATA3
T11428 23463-23472 Negative_regulation denotes Represses true
T11429 23477-23482 Protein denotes FOXP3 true
T11430 23483-23491 Entity denotes Promoter
T11431 23534-23539 Protein denotes GATA3
T11432 23540-23548 Positive_regulation denotes mediated
T11433 23549-23559 Negative_regulation denotes repression
T11434 23569-23574 Protein denotes FOXP3 true
T11435 23586-23591 Protein denotes FOXP3
T11436 23592-23600 Entity denotes promoter
T11437 23648-23653 Protein denotes GATA3
T11438 23678-23686 Entity denotes -binding
T11439 23874-23878 Regulation denotes role true
T11440 23882-23887 Protein denotes FOXP3
T11441 23888-23898 Regulation denotes regulation
T11442 23958-23963 Protein denotes FOXP3
T11443 24018-24021 Protein denotes CD4
T11444 24126-24133 Gene_expression denotes express
T11445 24134-24139 Protein denotes GATA3
T11446 24166-24171 Protein denotes FOXP3
T11447 24172-24180 Entity denotes promoter
T11448 24226-24231 Protein denotes GATA3
T11449 24232-24242 Gene_expression denotes expressing
T11450 24345-24348 Protein denotes CD4
T11451 24362-24369 Gene_expression denotes express
T11452 24388-24393 Protein denotes GATA3
T11453 24407-24421 Positive_regulation denotes Overexpression
T11454 24425-24430 Protein denotes GATA3
T11455 24448-24458 Negative_regulation denotes diminished
T11456 24486-24491 Protein denotes FOXP3
T11457 24492-24500 Entity denotes promoter
T11458 24635-24643 Negative_regulation denotes deleting
T11459 24653-24666 Entity denotes -binding site
T11460 24682-24691 Positive_regulation denotes increased
T11461 24732-24735 Protein denotes CD4
T11462 24736-24742 Protein denotes CD45RO
T11463 24798-24803 Protein denotes GATA3
T11464 24806-24809 Protein denotes CD4
T11465 24810-24816 Protein denotes CD45RA
T11466 24861-24866 Protein denotes GATA3
T11467 24874-24879 Protein denotes FOXP3
T11468 24880-24888 Entity denotes promoter
T11469 24914-24919 Protein denotes GATA3
T11470 24920-24925 Binding denotes binds
T11471 24942-24947 Protein denotes FOXP3
T11472 24948-24956 Entity denotes promoter
T11473 25037-25042 Protein denotes GATA3
T11474 25168-25173 Protein denotes FOXP3
T11475 25268-25273 Protein denotes GATA3
T11476 25274-25281 Binding denotes binding
T11477 25323-25328 Protein denotes GATA3
T11478 25329-25339 Gene_expression denotes expressing
T11479 25414-25428 Gene_expression denotes overexpressing
T11480 25429-25434 Protein denotes GATA3
T11481 25456-25461 Protein denotes GATA3
T11482 25462-25469 Binding denotes binding
T11483 25535-25540 Protein denotes GATA3
T11484 25541-25546 Binding denotes binds
T11485 25563-25568 Protein denotes FOXP3
T11486 25569-25577 Entity denotes promoter
T11487 25690-25695 Protein denotes GATA3
T11488 25721-25726 Protein denotes GATA3
T11489 25727-25732 Binding denotes binds
T11490 25740-25745 Protein denotes FOXP3
T11491 25746-25754 Entity denotes promoter
T11492 25755-25761 Entity denotes region
T11493 25859-25864 Protein denotes GATA3
T11494 25865-25872 Binding denotes binding
T11495 25880-25885 Protein denotes FOXP3
T11496 25886-25894 Entity denotes promoter
T11497 25898-25908 Negative_regulation denotes repressing
T11498 25909-25914 Protein denotes FOXP3
T11499 25915-25925 Gene_expression denotes expression
T13229 27656-27661 Protein denotes FOXP3
T13230 27662-27671 Positive_regulation denotes induction
T13231 27715-27724 Negative_regulation denotes inhibited
T13232 27728-27733 Protein denotes GATA3
T13233 27882-27887 Protein denotes FOXP3
T13234 27948-27951 Protein denotes CD4
T13235 27952-27956 Protein denotes CD25
T13236 27977-27988 Positive_regulation denotes up-regulate
T13237 27989-27994 Protein denotes FOXP3
T13238 28100-28105 Protein denotes FOXP3
T13239 28106-28115 Positive_regulation denotes induction
T13240 28246-28257 Positive_regulation denotes up-regulate
T13241 28258-28263 Protein denotes FOXP3
T13242 28282-28287 Protein denotes TGF-β true
T13243 28338-28343 Protein denotes FOXP3 true
T13244 28481-28486 Protein denotes T-bet
T13245 28491-28496 Protein denotes GATA3
T13246 28625-28630 Protein denotes FOXP3
T13247 28783-28788 Protein denotes TGF-β
T13248 28804-28813 Positive_regulation denotes mandatory
T13249 28822-28831 Positive_regulation denotes induction
T13250 28835-28840 Protein denotes FOXP3
T13251 28866-28876 Gene_expression denotes expression
T13252 28880-28885 Protein denotes GATA3
T13253 28890-28895 Protein denotes T-bet
T13254 29005-29009 Protein denotes IL-4
T13255 29011-29016 Protein denotes IL-12
T13256 29021-29026 Protein denotes TGF-β
T13257 29051-29056 Protein denotes FOXP3
T13258 29057-29067 Gene_expression denotes expression true
T13259 29107-29112 Protein denotes FOXP3
T13260 29113-29123 Gene_expression denotes expressing true
T13261 29135-29140 Protein denotes GATA3
T13262 29145-29150 Protein denotes T-bet true
T13263 29156-29168 Positive_regulation denotes up-regulated
T13264 29243-29248 Protein denotes FOXP3
T13265 29249-29258 Positive_regulation denotes promoting
T13266 29296-29302 Protein denotes CTLA-4
T13267 29347-29357 Negative_regulation denotes inhibitors
T13268 29361-29366 Protein denotes GATA3 true
T13269 29367-29377 Gene_expression denotes expression
T13270 29397-29402 Protein denotes GATA3 true
T13271 29460-29468 Negative_regulation denotes inhibits
T13272 29469-29474 Protein denotes FOXP3 true
T13273 29475-29485 Gene_expression denotes expression true
T13274 29536-29540 Protein denotes IL-4
T13275 29549-29554 Protein denotes IL-13 true
T13276 29586-29593 Negative_regulation denotes inhibit
T13277 29594-29599 Protein denotes TGF-β
T13278 29600-29608 Positive_regulation denotes mediated
T13279 29609-29614 Protein denotes FOXP3
T13280 29615-29624 Positive_regulation denotes induction
T13281 29725-29729 Protein denotes IL-4
T13282 29800-29804 Protein denotes IL-4
T13283 29814-29818 Protein denotes IL-4
T13284 29902-29906 Protein denotes IL-4
T13285 29907-29911 Protein denotes IL-4
T13286 29991-29995 Protein denotes CD25
T13287 30001-30006 Protein denotes Foxp3
T13288 30061-30065 Protein denotes IL-4
T13289 30259-30266 Gene_expression denotes express
T13290 30267-30271 Protein denotes CD25
T13291 30313-30317 Protein denotes IL-4
T13292 30327-30334 Negative_regulation denotes inhibit
T13293 30335-30340 Protein denotes Foxp3
T13294 30341-30351 Gene_expression denotes expression
T13295 30406-30416 Positive_regulation denotes contribute true
T13296 30433-30437 Negative_regulation denotes drop
T13297 30441-30446 Protein denotes Foxp3
T13298 30447-30457 Gene_expression denotes expression
T13299 30459-30463 Protein denotes IL-4
T13300 30559-30564 Protein denotes IL-17
T13301 30565-30574 Gene_expression denotes producing true
T13302 30648-30652 Protein denotes IL-6
T13303 30675-30680 Protein denotes TGF-β true
T13304 30855-30864 Regulation denotes regulated
T13305 30891-30895 Protein denotes IL-4
T13306 30900-30904 Protein denotes IL-6
T13307 30906-30910 Protein denotes IL-4
T13308 30965-30970 Protein denotes FOXP3
T13309 30990-30993 Protein denotes CD4
T13310 30994-30998 Protein denotes CD25
T13311 31050-31054 Protein denotes IL-4
T13312 31095-31099 Protein denotes IL-4
T13313 31130-31138 Positive_regulation denotes enhanced
T13314 31139-31144 Protein denotes FOXP3
T13315 31145-31155 Gene_expression denotes expression
T13316 31214-31220 Positive_regulation denotes induce
T13317 31221-31226 Protein denotes GATA3
T13318 31227-31237 Gene_expression denotes expression
T13319 31239-31243 Protein denotes IL-4
T13320 31300-31308 Regulation denotes regulate
T13321 31309-31314 Protein denotes FOXP3
T13322 31315-31325 Gene_expression denotes expression
T13323 31366-31371 Protein denotes IL-13
T13324 31393-31399 Negative_regulation denotes reduce
T13325 31400-31405 Protein denotes FOXP3
T13326 31419-31425 Positive_regulation denotes induce
T13327 31426-31431 Protein denotes GATA3
T13328 31458-31462 Protein denotes IL-4
T13329 31463-31472 Regulation denotes dependent
T13330 31473-31483 Negative_regulation denotes inhibition
T13331 31487-31492 Protein denotes FOXP3
T13332 31502-31510 Positive_regulation denotes mediated
T13333 31514-31519 Protein denotes GATA3
T13334 31530-31535 Protein denotes GATA3
T13335 31536-31544 Positive_regulation denotes inducing
T13336 31545-31549 Protein denotes IL-4
T13337 31565-31574 Negative_regulation denotes repressed
T13338 31575-31580 Protein denotes TGF-β
T13339 31581-31589 Positive_regulation denotes mediated
T13340 31590-31595 Protein denotes FOXP3
T13341 31596-31606 Gene_expression denotes expression
T13342 31616-31620 Protein denotes IL-4
T13343 31632-31637 Protein denotes TGF-β
T13344 31715-31720 Protein denotes GATA3
T13345 31725-31730 Protein denotes FOXP3
T13346 31869-31873 Positive_regulation denotes high
T13347 31874-31879 Protein denotes GATA3
T13348 31880-31890 Gene_expression denotes expressing
T13349 31915-31922 Negative_regulation denotes absence
T13350 31926-31931 Protein denotes FOXP3
T13351 31957-31962 Protein denotes GATA3
T13352 31982-31991 Negative_regulation denotes inhibited
T13353 31992-31997 Protein denotes FOXP3
T13354 31998-32007 Positive_regulation denotes induction
T13355 32055-32065 Negative_regulation denotes inhibitory
T13356 32066-32072 Regulation denotes effect
T13357 32076-32081 Protein denotes GATA3
T13358 32131-32141 Gene_expression denotes expressing
T13359 32142-32147 Protein denotes GATA3
T13360 32168-32171 Protein denotes CD2
T13361 32172-32177 Protein denotes GATA3
T13362 32223-32237 Positive_regulation denotes overexpression
T13363 32241-32246 Protein denotes GATA3
T13364 32295-32301 Positive_regulation denotes induce
T13365 32302-32307 Protein denotes FOXP3
T13366 32308-32318 Gene_expression denotes expression
T13367 32365-32370 Protein denotes TGF-β
T13368 32396-32399 Protein denotes CD2
T13369 32400-32405 Protein denotes GATA3
T13370 32430-32435 Protein denotes FOXP3
T13371 32535-32540 Protein denotes GATA3
T13372 32775-32780 Protein denotes GATA3
T13373 32781-32795 Positive_regulation denotes overexpression
T13374 32851-32861 Negative_regulation denotes inhibiting
T13375 32874-32879 Protein denotes GATA3
T13376 32903-32908 Protein denotes GATA3
T13377 32982-33000 Protein denotes Repressor of GATA3
T13378 33002-33005 Protein denotes ROG
T13379 33045-33048 Protein denotes CD8
T13380 33196-33201 Protein denotes GATA3
T13381 33287-33292 Protein denotes GATA3
T13382 33293-33302 Negative_regulation denotes repressed
T13383 33303-33308 Protein denotes FOXP3
T13384 33309-33319 Gene_expression denotes expression
T13385 33332-33339 Binding denotes binding
T13386 33347-33352 Protein denotes FOXP3
T13387 33353-33361 Entity denotes promoter
T13388 33362-33368 Entity denotes region
T13389 33505-33510 Protein denotes FOXP3
T13390 33706-33713 Gene_expression denotes express
T13391 33714-33719 Protein denotes GATA3
T13392 33783-33790 Gene_expression denotes express
T13393 33791-33796 Protein denotes GATA3
T13394 33831-33836 Binding denotes bound
T13395 33840-33845 Protein denotes GATA3
T13396 33930-33935 Protein denotes GATA3
T13397 33936-33941 Binding denotes binds
T13398 33994-33999 Protein denotes GATA3
T13399 34004-34010 Positive_regulation denotes induce
T13400 34011-34024 Transcription denotes transcription
T13401 34203-34208 Protein denotes GATA3
T13402 34209-34219 Gene_expression denotes expression
T13403 34233-34241 Positive_regulation denotes required
T13404 34262-34267 Protein denotes FOXP3
T13405 34328-34333 Protein denotes GATA3
T13406 34337-34344 Negative_regulation denotes inhibit
T13407 34345-34350 Protein denotes FOXP3
T13408 34387-34395 Entity denotes -binding
T13409 34490-34495 Protein denotes GATA3
T13410 34500-34507 Negative_regulation denotes compete
T13411 34517-34524 Binding denotes binding
T13412 34528-34532 Protein denotes AP-1
T13413 34533-34537 Protein denotes NFAT
T13414 34545-34553 Entity denotes promoter
T13415 34615-34620 Protein denotes FOXP3
T13416 34635-34644 Regulation denotes regulated
T13417 34666-34670 Protein denotes IL-4
T13418 34672-34677 Protein denotes GATA3
T13419 34689-34698 Negative_regulation denotes inhibitor
T13420 34702-34707 Protein denotes FOXP3
T13421 34708-34718 Gene_expression denotes expression
T13422 34803-34810 Binding denotes binding
T13423 34815-34825 Negative_regulation denotes repressing
T13424 34830-34835 Protein denotes FOXP3
T13425 34836-34844 Entity denotes promoter
T13426 34891-34895 Protein denotes Il-4
T13427 34913-34922 Positive_regulation denotes induction
T13428 34926-34936 Negative_regulation denotes repressing
T13429 34937-34942 Protein denotes FOXP3
T13430 34943-34953 Gene_expression denotes expression
T17758 35386-35396 Gene_expression denotes expressing
T17759 35397-35403 Protein denotes GATA-3
T17760 35425-35428 Protein denotes CD2
T17761 35451-35454 Protein denotes CD2
T17762 35455-35460 Protein denotes GATA3
T17763 35486-35492 Protein denotes 10xCD2
T17764 35493-35498 Protein denotes GATA3
T18058 35795-35798 Protein denotes CD4
T18059 35809-35812 Protein denotes CD4
T18060 35890-35893 Protein denotes CD4
T18061 35979-35982 Protein denotes CD4
T18062 36177-36181 Positive_regulation denotes high
T18063 36182-36186 Protein denotes IL-4
T18064 36187-36196 Localization denotes secretion
T18312 36389-36394 Protein denotes FOXP3
T18313 36448-36453 Protein denotes FOXP3
T18314 36499-36504 Protein denotes EF-1α
T18315 36558-36563 Protein denotes GATA3
T18316 36977-36988 Protein denotes Ubiquitin C
T18317 37062-37068 Protein denotes Smad-7
T18318 37141-37147 Protein denotes TGF-ß1
T18319 37568-37579 Protein denotes ubiquitin C
T18769 37820-37823 Protein denotes CD4 true
T18770 37834-37837 Protein denotes CD4
T18771 37840-37845 Protein denotes CD62L
T18772 37852-37856 Protein denotes CD25
T18773 38123-38126 Protein denotes -ß1
T18774 38221-38226 Protein denotes FOXP3
T18775 38227-38237 Gene_expression denotes expression
T19074 38323-38326 Protein denotes CD4
T19075 38328-38334 Protein denotes CD45RA
T19076 38357-38363 Protein denotes CD45RO
T19077 38364-38373 Negative_regulation denotes depletion
T19078 38484-38487 Protein denotes CD3
T19079 38519-38523 Protein denotes CD28
T19080 38562-38567 Protein denotes IL-12
T19081 38582-38586 Protein denotes IL-4
T19082 38630-38634 Protein denotes IL-4
T19083 38649-38654 Protein denotes IFN-γ
T19084 38669-38674 Protein denotes IL-12
T19085 38722-38727 Protein denotes TGF-β
T19086 38742-38747 Protein denotes IFN-γ
T19087 38762-38767 Protein denotes IL-12
T19088 38782-38786 Protein denotes IL-4
T19089 38843-38847 Protein denotes IL-2
T19507 38876-38881 Protein denotes FOXP3
T19508 38934-38939 Protein denotes FOXP3
T19509 38940-38948 Entity denotes promoter
T19510 39026-39031 Protein denotes FOXP3
T19511 39081-39086 Protein denotes FOXP3
T19747 39467-39472 Protein denotes FOXP3
T19748 39482-39492 Protein denotes luciferase
T19749 39550-39553 Protein denotes CD4
T19750 39779-39789 Protein denotes luciferase
T19751 39824-39834 Protein denotes luciferase
T19752 39960-39970 Protein denotes luciferase
T20157 40294-40297 Protein denotes TAT
T20158 40327-40332 Protein denotes GATA3
T20159 40358-40363 Protein denotes GATA3
T20160 40365-40372 Negative_regulation denotes lacking
T20161 40377-40393 Entity denotes two zinc fingers
T20162 40457-40460 Protein denotes TAT
T20163 40631-40634 Protein denotes TAT
T20417 40747-40750 Protein denotes TAT true
T20418 40751-40756 Protein denotes GATA3
T20419 40771-40774 Protein denotes CD4
T20420 40775-40781 Protein denotes CD45RA
T20421 40890-40895 Protein denotes GATA3
T20422 40985-40988 Protein denotes CD3
T20423 40998-41002 Protein denotes CD28
T20424 41007-41012 Protein denotes TGF-β
T20425 41039-41042 Protein denotes TAT
T20426 41086-41091 Protein denotes FOXP3
T20427 41092-41102 Gene_expression denotes expression
T20708 41316-41320 Protein denotes IL-4
T20912 41610-41615 Protein denotes FOXP3 true
T20913 41616-41626 Gene_expression denotes expression true
T20914 41707-41711 Protein denotes CD25
T20915 41851-41856 Protein denotes FOXP3
T20916 42081-42086 Protein denotes CD152
T20917 42109-42113 Protein denotes PD-1
T20918 42135-42139 Protein denotes GITR
T20919 42162-42166 Protein denotes CD69
T20920 42191-42196 Protein denotes CD103
T20921 42220-42225 Protein denotes CD62L
T20922 42483-42486 Protein denotes CD3
T20923 42493-42496 Protein denotes CD4
T20924 42507-42511 Protein denotes CD25
T20925 42646-42649 Protein denotes CD4
T20926 42686-42692 Protein denotes 10xCD2
T20927 42693-42698 Protein denotes GATA3
T20928 42737-42741 Protein denotes CD25
T20929 42753-42758 Protein denotes CD62L
T20930 42772-42775 Protein denotes CD4
T20931 42885-42890 Protein denotes Foxp3
T20932 42891-42901 Gene_expression denotes expression
T20933 42975-42980 Protein denotes Foxp3
T20934 43050-43053 Protein denotes CD4
T21764 43267-43272 Protein denotes FOXP3
T21765 43312-43315 Protein denotes CD4
T21766 43316-43320 Protein denotes CD25
T21767 43651-43656 Protein denotes FOXP3
T21768 43941-43946 Protein denotes GATA3
T21769 43988-43993 Protein denotes T-bet
T21770 44033-44038 Protein denotes GAPDH
T21771 44067-44072 Protein denotes SMAD2
T21772 44095-44100 Protein denotes STAT6
T21773 44117-44122 Protein denotes STAT6
T22232 44335-44339 Protein denotes IL-4 true
T22233 44390-44392 Protein denotes -4 true
T22234 44409-44413 Protein denotes IL-4 true
T22235 44521-44524 Protein denotes CD3
T22236 44526-44529 Protein denotes CD4
T22237 44535-44539 Protein denotes CD25
T22238 44540-44550 Gene_expression denotes expression
T22239 44567-44571 Protein denotes IL-4
T22240 44636-44640 Protein denotes IL-4
T22561 45111-45116 Protein denotes GATA3
T22562 45490-45495 Protein denotes FOXP3
T22963 45716-45719 Protein denotes CD4
T22964 46599-46604 Protein denotes GATA3
T23439 7404-7410 Positive_regulation denotes Induce true
T23440 7411-7416 Protein denotes FOXP3
T23441 7417-7427 Gene_expression denotes Expression
T23442 7483-7486 Protein denotes CD3
T23443 7487-7491 Protein denotes CD28
T23444 7508-7513 Protein denotes TGF-β
T23445 7587-7592 Protein denotes FOXP3
T23794 10157-10164 Gene_expression denotes Express true
T23795 10165-10170 Protein denotes FOXP3 true
T23796 10206-10211 Protein denotes FOXP3 true
T23797 10212-10222 Gene_expression denotes expression
T23798 10342-10347 Protein denotes TGF-β
T23799 10349-10354 Protein denotes FOXP3
T23800 10355-10365 Gene_expression denotes expression
T24074 10632-10636 Protein denotes IL-4
T24075 10637-10646 Gene_expression denotes Producing
T24076 10653-10657 Negative_regulation denotes Lack true
T24077 10658-10663 Protein denotes FOXP3 true
T24078 10699-10704 Protein denotes FOXP3
T24079 10709-10713 Protein denotes IL-4
T24080 10714-10724 Gene_expression denotes expression
T24081 10725-10734 Positive_regulation denotes following
T24082 10762-10765 Protein denotes CD4
T24083 10802-10808 Protein denotes CD45RO
T24084 10813-10817 Protein denotes CD25
T24085 10826-10836 Gene_expression denotes expression
T24086 10892-10898 Protein denotes CD45RO
T24087 10899-10903 Protein denotes CD25
T24088 10926-10932 Protein denotes CD45RO
T24089 10933-10937 Protein denotes CD25
T24090 10962-10968 Protein denotes CD45RO
T24091 10969-10973 Protein denotes CD25
T24092 11243-11248 Protein denotes CRTH2
T24093 11261-11265 Protein denotes IL-4
T24094 11303-11309 Protein denotes CD45RO
T24095 11328-11333 Protein denotes FOXP3
T24096 11338-11342 Protein denotes IL-4
T24665 13213-13218 Protein denotes FOXP3
T24666 13219-13228 Positive_regulation denotes Induction true
T24667 13274-13277 Protein denotes CD4 true
T24668 13278-13284 Protein denotes CD45RA true
T24669 13331-13334 Protein denotes CD3 true
T24670 13335-13339 Protein denotes CD28
T24671 13359-13364 Protein denotes TGF-β
T24672 13378-13382 Protein denotes IL-4
T24673 13491-13496 Protein denotes FOXP3
T24674 13501-13506 Protein denotes GATA3
T24675 13507-13517 Gene_expression denotes expression
T24676 13596-13601 Protein denotes GATA3
T24677 13606-13611 Protein denotes FOXP3
T24678 13612-13620 Gene_expression denotes staining
T24679 13648-13651 Protein denotes CD4
T24680 13652-13658 Protein denotes CD45RA
T25090 16498-16504 Regulation denotes Effect
T25091 16508-16512 Protein denotes IL-4
T25092 16516-16521 Protein denotes FOXP3
T25093 16522-16531 Positive_regulation denotes Induction true
T25094 16599-16604 Protein denotes TGF-β true
T25095 16636-16640 Protein denotes IL-4
T25096 16869-16872 Protein denotes CD4
T25097 16873-16879 Protein denotes CD45RA
T25098 16949-16954 Protein denotes TGF-β
T25099 16973-16983 Positive_regulation denotes increasing
T25100 17001-17005 Protein denotes IL-4
T25101 17081-17084 Protein denotes CD4
T25102 17085-17091 Protein denotes CD45RA
T25103 17146-17149 Protein denotes CD3
T25104 17150-17154 Protein denotes CD28
T25105 17156-17161 Protein denotes TGF-β
T25106 17177-17181 Protein denotes IL-4
T25107 17279-17293 Phosphorylation denotes phosphorylated
T25108 17294-17299 Protein denotes SMAD2
T25109 17304-17309 Protein denotes STAT6
T25110 17317-17322 Protein denotes STAT6
T25111 17327-17332 Protein denotes GAPDH
T25112 17379-17384 Protein denotes GATA3
T25113 17389-17394 Protein denotes FOXP3
T25114 17395-17403 Gene_expression denotes staining
T25115 17432-17435 Protein denotes CD4
T25116 17436-17442 Protein denotes CD45RA
T25117 17455-17459 Protein denotes IL-4
T25839 18665-18669 Protein denotes IL-4 true
T25840 18670-18678 Negative_regulation denotes Inhibits true
T25841 18679-18684 Protein denotes TGF-β
T25842 18724-18727 Protein denotes CD4
T25843 18728-18734 Protein denotes CD45RA
T25844 18779-18782 Protein denotes CD3
T25845 18783-18787 Protein denotes CD28
T25846 18789-18794 Protein denotes TGF-β
T25847 18800-18804 Protein denotes IL-4
T25848 19101-19106 Protein denotes GATA3
T25849 19111-19116 Protein denotes FOXP3
T25850 19117-19125 Gene_expression denotes staining
T25851 19168-19171 Protein denotes CD4
T25852 19172-19178 Protein denotes CD45RA
T25853 19196-19199 Protein denotes CD3
T25854 19200-19202 Protein denotes 28
T25855 19204-19208 Protein denotes IL-4
T25856 19213-19218 Protein denotes TGF-β
T26290 22450-22455 Protein denotes GATA3 true
T26291 22466-22484 Negative_regulation denotes Negative Regulator true
T26292 22488-22493 Protein denotes FOXP3 true
T26293 22494-22504 Gene_expression denotes Expression true
T26294 22521-22524 Protein denotes CD4
T26295 22525-22529 Protein denotes CDRA
T26296 22586-22595 Protein denotes TAT-GATA3
T26297 22635-22640 Protein denotes GATA3
T26298 22680-22683 Protein denotes CD3
T26299 22684-22688 Protein denotes CD28
T26300 22714-22717 Protein denotes GFP
T26301 22775-22780 Protein denotes FOXP3
T26302 22781-22791 Gene_expression denotes expression
T26303 22792-22801 Positive_regulation denotes following
T26304 22902-22905 Protein denotes CD4
T26305 22906-22910 Protein denotes CD25
T26306 22956-22962 Protein denotes 10xCD2
T26307 22963-22968 Protein denotes GATA3
T26308 22999-23004 Protein denotes TGF-β
T26309 23023-23026 Protein denotes CD4
T26310 23045-23050 Protein denotes FOXP3
T26311 23230-23235 Protein denotes SMAD7
T26312 23240-23245 Protein denotes TGF-β
T26313 23246-23256 Gene_expression denotes expression
T26880 25937-25942 Protein denotes GATA3
T26881 25943-25952 Negative_regulation denotes Represses
T26882 25963-25968 Protein denotes FOXP3 true
T26883 25969-25977 Entity denotes Promoter true
T26884 26019-26022 Protein denotes CD4
T26885 26116-26121 Protein denotes FOXP3
T26886 26151-26161 Protein denotes luciferase
T26887 26249-26259 Protein denotes luciferase
T26888 26341-26344 Protein denotes CD4
T26889 26379-26384 Protein denotes FOXP3
T26890 26429-26434 Protein denotes GATA3
T26891 26435-26445 Gene_expression denotes expression
T26892 26578-26581 Protein denotes CD4
T26893 26627-26632 Protein denotes GATA3
T26894 26645-26650 Protein denotes FOXP3
T26895 26789-26799 Protein denotes luciferase
T26896 26939-26944 Protein denotes GATA3
T26897 27165-27170 Protein denotes GATA3
T26898 27241-27246 Protein denotes GATA3
T26899 27407-27412 Protein denotes GATA3
T26900 27413-27420 Binding denotes binding
T26901 27428-27433 Protein denotes FOXP3
T26902 27434-27442 Entity denotes promoter
R22 T31 T32 causeOf GATA3,Inhibit
R23 T33 T34 causeOf TGF-β1,Induced
R24 T34 T32 themeOf Induced,Inhibit
R25 T35 T36 themeOf FOXP3,Expression
R26 T36 T34 themeOf Expression,Induced
R27 T38 T40 causeOf interleukin 4,inhibits
R28 T39 T38 equivalentTo IL-4,interleukin 4
R29 T41 T40 themeOf FOXP3,inhibits
R30 T43 T42 themeOf GATA-3,overexpressing
R31 T47 T48 themeOf FOXP3,induction
R32 T48 T46 themeOf induction,mediated
R33 T50 T49 themeOf GATA3,binding
R34 T52 T51 partOf promoter,FOXP3
R35 T52 T49 themeOf promoter,binding
R36 T53 T54 themeOf IL-4,target
R37 T55 T54 themeOf IL-4,target
R38 T56 T54 themeOf GATA3,target
R711 T867 T869 causeOf interleukin 4,induced
R712 T868 T867 equivalentTo IL-4,interleukin 4
R713 T870 T869 themeOf STAT6,induced
R714 T871 T869 themeOf GATA3,induced
R715 T874 T873 themeOf IL-4,induction
R716 T875 T873 themeOf IL-5,induction
R717 T876 T873 themeOf IL-13,induction
R718 T877 T878 causeOf T-bet,modulates
R719 T879 T878 themeOf GATA3,modulates
R720 T886 T887 themeOf GATA3,blockade
R721 T887 T885 themeOf blockade,mediated
R722 T889 T888 themeOf FOXP3,express
R723 T891 T890 themeOf CD25,express
R724 T893 T892 themeOf FOXP3,express
R725 T895 T896 themeOf FOXP3,expression
R726 T896 T894 themeOf expression,induce
R727 T899 T898 themeOf TGF-βRII,lacking
R728 T905 T906 causeOf GATA3,inhibits
R729 T907 T908 themeOf FOXP3,induction
R730 T908 T906 themeOf induction,inhibits
R731 T910 T911 themeOf FOXP3,expression
R732 T914 T915 themeOf GATA3,binds
R733 T917 T916 partOf promoter,FOXP3
R734 T917 T915 themeOf promoter,binds
R2254 T2744 T2745 themeOf FOXP3,Induction
R2255 T2746 T2747 themeOf FOXP3,expression
R2256 T2747 T2748 themeOf expression,induced
R2257 T2751 T2750 themeOf FOXP3,express
R2258 T2752 T2753 themeOf FOXP3,expressed
R2259 T2752 T2754 themeOf FOXP3,expression
R2260 T2755 T2756 themeOf FOXP3,mRNA expression
R2261 T2769 T2768 themeOf FOXP3,induce
R2262 T2770 T2768 themeOf up to,induce
R2263 T2771 T2770 causeOf TGF-β,up to
R2264 T2773 T2774 themeOf FOXP3,expression
R2265 T2774 T2772 themeOf expression,increase
R2266 T2776 T2775 themeOf FOXP3,up-regulate
R2267 T2778 T2782 causeOf IL-4,prevent
R2268 T2778 T2777 themeOf IL-4,produce
R2269 T2778 T2780 themeOf IL-4,interact
R2270 T2780 T2782 causeOf interact,prevent
R2271 T2783 T2784 themeOf FOXP3,induction
R2272 T2784 T2782 themeOf induction,prevent
R2273 T2785 T2789 causeOf neutralization,rescue
R2274 T2786 T2785 themeOf IL-4,neutralization
R2275 T2790 T2791 themeOf FOXP3,expression
R2276 T2791 T2789 themeOf expression,rescue
R2277 T2793 T2792 themeOf FOXP3,express
R2278 T2795 T2794 themeOf FOXP3,express
R2279 T2797 T2798 themeOf FOXP3,expression
R2280 T2798 T2796 themeOf expression,lack
R2281 T2800 T2799 themeOf FOXP3,expressed
R2282 T2802 T2801 themeOf FOXP3,express
R2283 T2804 T2805 themeOf CD25,expression
R2284 T2805 T2803 themeOf expression,low
R2285 T2806 T2807 causeOf TGF-β,increase
R2286 T2808 T2809 themeOf FOXP3,expression
R2287 T2809 T2807 themeOf expression,increase
R2288 T2811 T2812 themeOf FOXP3,expression
R2289 T2812 T2810 themeOf expression,induced
R2290 T2814 T2815 themeOf IL-4,secretion
R2291 T2816 T2817 themeOf IL-4,producing
R2292 T2819 T2820 themeOf FOXP3,expression
R2293 T2820 T2818 themeOf expression,lacking
R2294 T2821 T2824 causeOf TGF-β,induction
R2295 T2821 T2825 causeOf TGF-β,failed
R2296 T2821 T2822 causeOf TGF-β,mediated
R2297 T2823 T2824 themeOf FOXP3,induction
R2298 T2824 T2822 themeOf induction,mediated
R2299 T2824 T2825 themeOf induction,failed
R2300 T2828 T2827 themeOf IL-4,expression
R2301 T2829 T2827 themeOf FOXP3,expression
R2302 T2831 T2832 themeOf IL-4,expressing
R2303 T2837 T2836 themeOf FOXP3,express
R2304 T2841 T2840 themeOf FOXP3,expressed
R2305 T2843 T2842 themeOf IL-4,lacking
R2306 T2856 T2855 themeOf FOXP3,expressed
R2307 T2857 T2858 themeOf IL-4,secretion
R2308 T2859 T2860 themeOf FOXP3,expressing
R2309 T2862 T2863 themeOf FOXP3,expressed
R2310 T2862 T2864 themeOf FOXP3,inducible
R2311 T2863 T2864 themeOf expressed,inducible
R3952 T5123 T5124 themeOf FOXP3,expression
R3953 T5124 T5122 themeOf expression,induce
R3954 T5127 T5126 themeOf FOXP3,expression
R3955 T5128 T5126 themeOf GATA3,expression
R3956 T5135 T5136 themeOf FOXP3,mRNA expression
R3957 T5136 T5137 themeOf mRNA expression,increased
R3958 T5138 T5140 themeOf GATA3,expression
R3959 T5139 T5140 themeOf FOXP3,expression
R3960 T5142 T5141 themeOf FOXP3,expressing
R3961 T5144 T5145 themeOf GATA3,expression
R3962 T5144 T5143 themeOf GATA3,expressed
R3963 T5148 T5152 themeOf CD103,expression
R3964 T5149 T5152 themeOf CTLA-4,expression
R3965 T5150 T5152 themeOf GITR,expression
R3966 T5151 T5152 themeOf PD-1,expression
R3967 T5153 T5155 themeOf GATA3,expression
R3968 T5154 T5155 themeOf FOXP3,expression
R3969 T5157 T5156 themeOf express,lost
R3970 T5158 T5157 themeOf FOXP3,express
R4831 T6295 T6296 causeOf IL-4,inhibit
R4832 T6297 T6298 causeOf TGF-β,induction
R4833 T6298 T6296 themeOf induction,inhibit
R4834 T6299 T6298 themeOf FOXP3,induction
R4835 T6306 T6307 causeOf IL-4,repressed
R4836 T6308 T6309 causeOf TGF-β,induction
R4837 T6310 T6311 themeOf FOXP3,expression
R4838 T6311 T6309 themeOf expression,induction
R4839 T6312 T6313 themeOf GATA3,induced
R4840 T6314 T6313 causeOf absence,induced
R4841 T6315 T6314 themeOf IL-4,absence
R4842 T6316 T6317 causeOf IL-4,induce
R4843 T6318 T6319 themeOf FOXP3,expression
R4844 T6319 T6317 themeOf expression,induce
R4845 T6320 T6321 themeOf GATA3,induced
R4846 T6322 T6321 causeOf TGF-β,induced
R4847 T6323 T6321 causeOf high,induced
R4848 T6324 T6321 causeOf IL-4,induced
R4849 T6325 T6326 causeOf IL-4,prevention
R4850 T6326 T6329 themeOf prevention,caused
R4851 T6327 T6328 themeOf FOXP3,expression
R4852 T6328 T6326 themeOf expression,prevention
R4853 T6328 T6329 themeOf expression,caused
R4854 T6330 T6333 themeOf phosphorylation,affected
R4855 T6331 T6330 themeOf SMAD2,phosphorylation
R4856 T6332 T6330 themeOf STAT6,phosphorylation
R4857 T6334 T6333 causeOf IL-4,affected
R4858 T6335 T6333 causeOf TGF-β,affected
R4859 T6340 T6339 themeOf GATA3,increase
R4860 T6341 T6342 themeOf FOXP3,decreased
R4861 T6345 T6344 themeOf CD25,express
R4862 T6347 T6346 equivalentTo rmIL-4,IL-4
R4863 T6364 T6363 themeOf IL-4,effects
R4864 T6367 T6368 themeOf FOXP3,expression
R4865 T6369 T6370 causeOf IL-4,inhibit
R4866 T6371 T6372 themeOf FOXP3,expression
R4867 T6372 T6370 themeOf expression,inhibit
R4868 T6374 T6375 themeOf FOXP3,expression
R4869 T6375 T6373 themeOf expression,decrease
R4870 T6376 T6373 causeOf IL-4,decrease
R6247 T8198 T8199 causeOf TGF-β,reduced
R6248 T8200 T8202 themeOf CD25,expression
R6249 T8201 T8202 themeOf IL-4,expression
R6250 T8202 T8199 themeOf expression,reduced
R6251 T8203 T8204 themeOf CD25,expression
R6252 T8204 T8199 themeOf expression,reduced
R6253 T8205 T8206 causeOf IL-4,inhibited
R6254 T8207 T8209 causeOf TGF-β,induction
R6255 T8207 T8208 causeOf TGF-β,mediated
R6256 T8209 T8206 themeOf induction,inhibited
R6257 T8210 T8209 themeOf FOXP3,induction
R6258 T8219 T8220 causeOf TGF-β,induction
R6259 T8220 T8223 themeOf induction,caused
R6260 T8221 T8222 themeOf FOXP3,expression
R6261 T8222 T8220 themeOf expression,induction
R6262 T8230 T8231 themeOf CD25,down-regulated
R6263 T8232 T8231 causeOf TGF-β,down-regulated
R6264 T8233 T8231 causeOf TGF-β,down-regulated
R6265 T8234 T8231 causeOf IL-4,down-regulated
R6266 T8237 T8238 themeOf IL-4,producing
R6267 T8239 T8240 themeOf FOXP3,expressing
R6268 T8242 T8244 themeOf GATA3,expression
R6269 T8243 T8244 themeOf FOXP3,expression
R6270 T8247 T8249 themeOf GATA3,co-express
R6271 T8248 T8249 themeOf FOXP3,co-express
R6272 T8250 T8251 causeOf GATA3,repressing
R6273 T8250 T8249 themeOf GATA3,co-express
R6274 T8252 T8251 themeOf FOXP3,repressing
R6275 T8253 T8254 causeOf IL-4,in vitro as
R6276 T8256 T8257 themeOf FOXP3,expression
R6277 T8257 T8255 themeOf expression,an inhibitor
R7339 T9667 T9668 themeOf FOXP3,Expression
R7340 T9669 T9670 themeOf FOXP3,expression
R7341 T9670 T9671 themeOf expression,decreased
R7342 T9672 T9673 themeOf GATA3,expression
R7343 T9673 T9671 causeOf expression,decreased
R7344 T9675 T9674 causeOf GATA3,role
R7345 T9677 T9676 themeOf FOXP3,repressing
R7346 T9678 T9680 causeOf GATA3,inhibiting
R7347 T9679 T9680 causeOf GATA3,inhibiting
R7348 T9681 T9682 themeOf STAT4,expression
R7349 T9682 T9680 themeOf expression,inhibiting
R7350 T9684 T9685 causeOf GATA3,inhibit
R7351 T9686 T9687 themeOf FOXP3,induction
R7352 T9687 T9685 themeOf induction,inhibit
R7353 T9689 T9688 themeOf GATA3,transduced
R7354 T9693 T9691 partOf DNA-binding domain,GATA3
R7355 T9693 T9692 themeOf DNA-binding domain,lacking
R7356 T9700 T9699 themeOf TGF-β,absence
R7357 T9703 T9704 causeOf TAT-GATA3,reduced
R7358 T9704 T9707 themeOf reduced,dependent
R7359 T9705 T9706 themeOf FOXP3,expression
R7360 T9706 T9704 themeOf expression,reduced
R7361 T9708 T9709 partOf DNA-binding domain,GATA3
R7362 T9711 T9712 themeOf FOXP3,expression
R7363 T9712 T9710 themeOf expression,affect
R7364 T9718 T9717 themeOf GATA3,overexpressing
R7365 T9729 T9731 themeOf TGF-β,expression
R7366 T9730 T9731 themeOf Foxp3,expression
R7367 T9735 T9736 causeOf TGF-β,up-regulated
R7368 T9737 T9736 themeOf Foxp3,up-regulated
R7369 T9739 T9740 causeOf GATA3xDO11,reduced
R7370 T9741 T9742 themeOf Foxp3,expression
R7371 T9742 T9740 themeOf expression,reduced
R7372 T9742 T9743 themeOf expression,activated
R7373 T9744 T9743 causeOf TGF-β,activated
R7374 T9746 T9745 themeOf TGF-β,produced
R7375 T9747 T9745 themeOf Smad7,produced
R7376 T9747 T9748 themeOf Smad7,expressed
R7377 T9750 T9751 causeOf IL-4,induced
R7378 T9752 T9751 themeOf GATA3,induced
R8544 T11427 T11428 causeOf GATA3,Represses
R8545 T11430 T11429 partOf Promoter,FOXP3
R8546 T11430 T11428 themeOf Promoter,Represses
R8547 T11431 T11433 causeOf GATA3,repression
R8548 T11431 T11432 causeOf GATA3,mediated
R8549 T11433 T11432 themeOf repression,mediated
R8550 T11434 T11433 themeOf FOXP3,repression
R8551 T11436 T11433 themeOf promoter,repression
R8552 T11436 T11435 partOf promoter,FOXP3
R8553 T11440 T11441 themeOf FOXP3,regulation
R8554 T11445 T11444 themeOf GATA3,express
R8555 T11447 T11446 partOf promoter,FOXP3
R8556 T11448 T11449 themeOf GATA3,expressing
R8557 T11452 T11451 themeOf GATA3,express
R8558 T11454 T11453 themeOf GATA3,Overexpression
R8559 T11457 T11456 partOf promoter,FOXP3
R8560 T11459 T11458 themeOf -binding site,deleting
R8561 T11468 T11467 partOf promoter,FOXP3
R8562 T11469 T11470 themeOf GATA3,binds
R8563 T11472 T11470 themeOf promoter,binds
R8564 T11472 T11471 partOf promoter,FOXP3
R8565 T11475 T11476 themeOf GATA3,binding
R8566 T11477 T11478 themeOf GATA3,expressing
R8567 T11480 T11479 themeOf GATA3,overexpressing
R8568 T11481 T11482 themeOf GATA3,binding
R8569 T11483 T11484 themeOf GATA3,binds
R8570 T11486 T11484 themeOf promoter,binds
R8571 T11486 T11485 partOf promoter,FOXP3
R8572 T11488 T11489 themeOf GATA3,binds
R8573 T11491 T11490 partOf promoter,FOXP3
R8574 T11491 T11489 themeOf promoter,binds
R8575 T11493 T11494 themeOf GATA3,binding
R8576 T11494 T11497 causeOf binding,repressing
R8577 T11496 T11494 themeOf promoter,binding
R8578 T11496 T11495 partOf promoter,FOXP3
R8579 T11498 T11499 themeOf FOXP3,expression
R8580 T11499 T11497 themeOf expression,repressing
R9855 T13229 T13230 themeOf FOXP3,induction
R9856 T13230 T13231 themeOf induction,inhibited
R9857 T13232 T13231 causeOf GATA3,inhibited
R9858 T13234 T13236 causeOf CD4,up-regulate
R9859 T13235 T13236 causeOf CD25,up-regulate
R9860 T13237 T13236 themeOf FOXP3,up-regulate
R9861 T13238 T13239 themeOf FOXP3,induction
R9862 T13241 T13240 themeOf FOXP3,up-regulate
R9863 T13242 T13240 causeOf TGF-β,up-regulate
R9864 T13249 T13248 themeOf induction,mandatory
R9865 T13250 T13249 themeOf FOXP3,induction
R9866 T13252 T13251 themeOf GATA3,expression
R9867 T13253 T13251 themeOf T-bet,expression
R9868 T13257 T13258 themeOf FOXP3,expression
R9869 T13259 T13260 themeOf FOXP3,expressing
R9870 T13261 T13263 themeOf GATA3,up-regulated
R9871 T13262 T13263 themeOf T-bet,up-regulated
R9872 T13264 T13265 themeOf FOXP3,promoting
R9873 T13266 T13265 causeOf CTLA-4,promoting
R9874 T13268 T13269 themeOf GATA3,expression
R9875 T13269 T13267 themeOf expression,inhibitors
R9876 T13270 T13271 causeOf GATA3,inhibits
R9877 T13272 T13273 themeOf FOXP3,expression
R9878 T13273 T13271 themeOf expression,inhibits
R9879 T13274 T13276 causeOf IL-4,inhibit
R9880 T13275 T13276 causeOf IL-13,inhibit
R9881 T13277 T13278 causeOf TGF-β,mediated
R9882 T13277 T13280 causeOf TGF-β,induction
R9883 T13279 T13280 themeOf FOXP3,induction
R9884 T13280 T13276 themeOf induction,inhibit
R9885 T13280 T13278 themeOf induction,mediated
R9886 T13289 T13292 causeOf express,inhibit
R9887 T13290 T13289 themeOf CD25,express
R9888 T13291 T13292 causeOf IL-4,inhibit
R9889 T13293 T13294 themeOf Foxp3,expression
R9890 T13294 T13292 themeOf expression,inhibit
R9891 T13297 T13298 themeOf Foxp3,expression
R9892 T13298 T13296 themeOf expression,drop
R9893 T13300 T13301 themeOf IL-17,producing
R9894 T13312 T13313 causeOf IL-4,enhanced
R9895 T13314 T13315 themeOf FOXP3,expression
R9896 T13315 T13313 themeOf expression,enhanced
R9897 T13317 T13318 themeOf GATA3,expression
R9898 T13318 T13316 themeOf expression,induce
R9899 T13319 T13320 causeOf IL-4,regulate
R9900 T13321 T13322 themeOf FOXP3,expression
R9901 T13322 T13320 themeOf expression,regulate
R9902 T13323 T13324 causeOf IL-13,reduce
R9903 T13323 T13326 causeOf IL-13,induce
R9904 T13325 T13324 themeOf FOXP3,reduce
R9905 T13327 T13326 themeOf GATA3,induce
R9906 T13328 T13330 causeOf IL-4,inhibition
R9907 T13328 T13329 causeOf IL-4,dependent
R9908 T13329 T13332 themeOf dependent,mediated
R9909 T13330 T13332 themeOf inhibition,mediated
R9910 T13331 T13330 themeOf FOXP3,inhibition
R9911 T13333 T13332 causeOf GATA3,mediated
R9912 T13334 T13337 causeOf GATA3,repressed
R9913 T13334 T13335 causeOf GATA3,inducing
R9914 T13336 T13335 themeOf IL-4,inducing
R9915 T13339 T13337 themeOf mediated,repressed
R9916 T13340 T13341 themeOf FOXP3,expression
R9917 T13341 T13339 themeOf expression,mediated
R9918 T13347 T13348 themeOf GATA3,expressing
R9919 T13348 T13346 themeOf expressing,high
R9920 T13350 T13349 themeOf FOXP3,absence
R9921 T13353 T13354 themeOf FOXP3,induction
R9922 T13354 T13352 themeOf induction,inhibited
R9923 T13359 T13358 themeOf GATA3,expressing
R9924 T13363 T13362 themeOf GATA3,overexpression
R9925 T13365 T13366 themeOf FOXP3,expression
R9926 T13366 T13364 themeOf expression,induce
R9927 T13372 T13373 themeOf GATA3,overexpression
R9928 T13375 T13374 themeOf GATA3,inhibiting
R9929 T13378 T13377 equivalentTo ROG,Repressor of GATA3
R9930 T13381 T13382 causeOf GATA3,repressed
R9931 T13381 T13385 themeOf GATA3,binding
R9932 T13383 T13384 themeOf FOXP3,expression
R9933 T13384 T13382 themeOf expression,repressed
R9934 T13385 T13382 causeOf binding,repressed
R9935 T13391 T13390 themeOf GATA3,express
R9936 T13393 T13392 themeOf GATA3,express
R9937 T13395 T13394 themeOf GATA3,bound
R9938 T13396 T13397 themeOf GATA3,binds
R9939 T13400 T13399 themeOf transcription,induce
R9940 T13401 T13402 themeOf GATA3,expression
R9941 T13402 T13403 causeOf expression,required
R9942 T13405 T13406 causeOf GATA3,inhibit
R9943 T13407 T13406 themeOf FOXP3,inhibit
R9944 T13409 T13410 causeOf GATA3,compete
R9945 T13411 T13410 themeOf binding,compete
R9946 T13412 T13411 themeOf AP-1,binding
R9947 T13413 T13411 themeOf NFAT,binding
R9948 T13414 T13407 partOf promoter,FOXP3
R9949 T13414 T13411 themeOf promoter,binding
R9950 T13417 T13416 causeOf IL-4,regulated
R9951 T13418 T13422 themeOf GATA3,binding
R9952 T13418 T13423 causeOf GATA3,repressing
R9953 T13420 T13421 themeOf FOXP3,expression
R9954 T13421 T13419 themeOf expression,inhibitor
R9955 T13425 T13424 partOf promoter,FOXP3
R9956 T13425 T13423 themeOf promoter,repressing
R9957 T13429 T13430 themeOf FOXP3,expression
R9958 T13430 T13428 themeOf expression,repressing
R13416 T17759 T17758 themeOf GATA-3,expressing
R13645 T18063 T18064 themeOf IL-4,secretion
R13646 T18064 T18062 themeOf secretion,high
R14157 T18774 T18775 themeOf FOXP3,expression
R14369 T19076 T19077 themeOf CD45RO,depletion
R14623 T19509 T19508 partOf promoter,FOXP3
R15107 T20161 T20160 themeOf two zinc fingers,lacking
R15108 T20161 T20159 partOf two zinc fingers,GATA3
R15289 T20426 T20427 themeOf FOXP3,expression
R15615 T20912 T20913 themeOf FOXP3,expression
R15616 T20931 T20932 themeOf Foxp3,expression
R16564 T22235 T22238 themeOf CD3,expression
R16565 T22236 T22238 themeOf CD4,expression
R16566 T22237 T22238 themeOf CD25,expression
R17591 T23440 T23441 themeOf FOXP3,Expression
R17592 T23441 T23439 themeOf Expression,Induce
R17838 T23795 T23794 themeOf FOXP3,Express
R17839 T23796 T23797 themeOf FOXP3,expression
R17840 T23799 T23800 themeOf FOXP3,expression
R18047 T24074 T24075 themeOf IL-4,Producing
R18048 T24077 T24076 themeOf FOXP3,Lack
R18049 T24078 T24080 themeOf FOXP3,expression
R18050 T24079 T24080 themeOf IL-4,expression
R18051 T24083 T24085 themeOf CD45RO,expression
R18052 T24084 T24085 themeOf CD25,expression
R18433 T24665 T24666 themeOf FOXP3,Induction
R18434 T24673 T24675 themeOf FOXP3,expression
R18435 T24674 T24675 themeOf GATA3,expression
R18436 T24676 T24678 themeOf GATA3,staining
R18437 T24677 T24678 themeOf FOXP3,staining
R18693 T25091 T25090 causeOf IL-4,Effect
R18694 T25092 T25093 themeOf FOXP3,Induction
R18695 T25093 T25090 themeOf Induction,Effect
R18696 T25100 T25099 themeOf IL-4,increasing
R18697 T25108 T25107 themeOf SMAD2,phosphorylated
R18698 T25109 T25107 themeOf STAT6,phosphorylated
R18699 T25112 T25114 themeOf GATA3,staining
R18700 T25113 T25114 themeOf FOXP3,staining
R19185 T25848 T25850 themeOf GATA3,staining
R19186 T25849 T25850 themeOf FOXP3,staining
R19442 T26292 T26293 themeOf FOXP3,Expression
R19443 T26293 T26291 themeOf Expression,Negative Regulator
R19444 T26301 T26302 themeOf FOXP3,expression
R19445 T26311 T26313 themeOf SMAD7,expression
R19446 T26312 T26313 themeOf TGF-β,expression
R19803 T26880 T26881 causeOf GATA3,Represses
R19804 T26883 T26881 themeOf Promoter,Represses
R19805 T26883 T26882 partOf Promoter,FOXP3
R19806 T26890 T26891 themeOf GATA3,expression
R19807 T26899 T26900 themeOf GATA3,binding
R19808 T26902 T26900 themeOf promoter,binding
R19809 T26902 T26901 partOf promoter,FOXP3