PMC:2195774 / 7071-7497 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2195774","sourcedb":"PMC","sourceid":"2195774","source_url":"http://www.ncbi.nlm.nih.gov/pmc/2195774","text":"Hap1 Activity in ydj1 Mutant Strains\nTo assay Hap1 activity, strain JJ261 expressing wild-type YDJ1, ydj1-104, or the indicated Ydj1–Sis1 fusion protein was transformed with a UAS1/CYC1-lacZ reporter plasmid (Guarente et al. 1984). After overnight incubation at 25°C in the presence of high concentrations of δ-aminolevulinic acid (ALA) (250 μg/ml), β-galactosidase assays were performed as described (Johnson and Craig 2000).","divisions":[{"label":"Title","span":{"begin":0,"end":36}}],"tracks":[]}