
PMC:2065877 / 47543-48609
Annnotations
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20344 | 0-5 | NN | denotes | Rnase |
T20345 | 6-16 | NN | denotes | protection |
T20346 | 17-22 | NN | denotes | assay |
T20347 | 24-29 | JJ | denotes | Total |
T20348 | 30-33 | NN | denotes | RNA |
T20349 | 34-37 | VB | denotes | was |
T20350 | 38-46 | VB | denotes | isolated |
T20351 | 47-51 | IN | denotes | from |
T20352 | 52-57 | JJ | denotes | CD19+ |
T20353 | 58-71 | JJ | denotes | MACS-purified |
T20354 | 72-73 | NN | denotes | B |
T20355 | 74-79 | NN | denotes | cells |
T20356 | 80-85 | VB | denotes | using |
T20357 | 86-89 | DT | denotes | the |
T20358 | 90-96 | NNP | denotes | Rneasy |
T20359 | 97-101 | NN | denotes | midi |
T20360 | 102-114 | NN | denotes | purification |
T20361 | 115-118 | NN | denotes | kit |
T20362 | 119-123 | IN | denotes | with |
T20363 | 124-129 | NN | denotes | Dnase |
T20364 | 130-139 | NN | denotes | treatment |
T20365 | 139-140 | -COMMA- | denotes | , |
T20366 | 141-150 | VB | denotes | according |
T20367 | 151-153 | TO | denotes | to |
T20368 | 154-157 | DT | denotes | the |
T20369 | 158-170 | NN | denotes | manufacturer |
T20370 | 170-172 | POS | denotes | 's |
T20371 | 173-185 | NN | denotes | instructions |
T20372 | 186-187 | -LRB- | denotes | ( |
T20373 | 187-193 | NN | denotes | Qiagen |
T20374 | 193-194 | -RRB- | denotes | ) |
T20375 | 196-199 | DT | denotes | The |
T20376 | 200-205 | NN | denotes | mCK-1 |
T20377 | 206-211 | NN | denotes | probe |
T20378 | 212-220 | NN | denotes | template |
T20379 | 221-224 | NN | denotes | set |
T20380 | 225-226 | -LRB- | denotes | ( |
T20381 | 226-228 | NN | denotes | BD |
T20382 | 229-240 | NNP | denotes | Biosciences |
T20383 | 240-241 | -RRB- | denotes | ) |
T20384 | 242-245 | VB | denotes | was |
T20385 | 246-253 | VB | denotes | labeled |
T20386 | 254-259 | VB | denotes | using |
T20387 | 260-263 | DT | denotes | the |
T20388 | 264-266 | FW | denotes | In |
T20389 | 267-272 | FW | denotes | Vitro |
T20390 | 273-286 | NN | denotes | Transcription |
T20391 | 287-290 | NN | denotes | Kit |
T20392 | 291-300 | VB | denotes | according |
T20393 | 301-303 | TO | denotes | to |
T20394 | 304-307 | DT | denotes | the |
T20395 | 308-320 | NN | denotes | manufacturer |
T20396 | 320-322 | POS | denotes | 's |
T20397 | 323-335 | NN | denotes | instructions |
T20398 | 336-337 | -LRB- | denotes | ( |
T20399 | 337-339 | NN | denotes | BD |
T20400 | 340-351 | NNP | denotes | Biosciences |
T20401 | 351-352 | -RRB- | denotes | ) |
T20402 | 354-361 | RB | denotes | Briefly |
T20403 | 361-362 | -COMMA- | denotes | , |
T20404 | 363-365 | CD | denotes | 50 |
T20405 | 366-368 | NN | denotes | ng |
T20406 | 369-371 | IN | denotes | of |
T20407 | 372-377 | NN | denotes | mCK-1 |
T20408 | 378-383 | NN | denotes | probe |
T20409 | 384-387 | NN | denotes | set |
T20410 | 388-391 | VB | denotes | was |
T20411 | 392-399 | VB | denotes | labeled |
T20412 | 400-404 | IN | denotes | with |
T20413 | 405-406 | -LRB- | denotes | [ |
T20414 | 406-411 | NN | denotes | α-32P |
T20415 | 411-412 | -RRB- | denotes | ] |
T20416 | 412-415 | NN | denotes | UTP |
T20417 | 416-421 | VB | denotes | using |
T20418 | 422-424 | NN | denotes | T7 |
T20419 | 425-428 | NN | denotes | RNA |
T20420 | 429-439 | NN | denotes | polymerase |
T20421 | 440-443 | CC | denotes | and |
T20422 | 444-452 | VB | denotes | purified |
T20423 | 453-458 | VB | denotes | using |
T20424 | 459-467 | NN | denotes | Sephadex |
T20425 | 468-472 | NN | denotes | G-50 |
T20426 | 473-480 | NN | denotes | columns |
T20427 | 481-482 | -LRB- | denotes | ( |
T20428 | 482-489 | NNP | denotes | NucAway |
T20429 | 490-494 | NNP | denotes | Spin |
T20430 | 495-501 | NN | denotes | column |
T20431 | 501-502 | -COMMA- | denotes | , |
T20432 | 503-509 | NNP | denotes | Ambion |
T20433 | 509-510 | -RRB- | denotes | ) |
T20434 | 512-515 | DT | denotes | The |
T20435 | 516-523 | VB | denotes | labeled |
T20436 | 524-529 | NN | denotes | probe |
T20437 | 530-533 | VB | denotes | was |
T20438 | 534-545 | VB | denotes | quantitated |
T20439 | 546-551 | VB | denotes | using |
T20440 | 552-553 | DT | denotes | a |
T20441 | 554-567 | NN | denotes | scintillation |
T20442 | 568-575 | NN | denotes | counter |
T20443 | 575-576 | -COMMA- | denotes | , |
T20444 | 577-580 | CC | denotes | and |
T20445 | 581-582 | CD | denotes | 6 |
T20446 | 583-584 | SYM | denotes | × |
T20447 | 585-588 | CD | denotes | 105 |
T20448 | 589-598 | NN | denotes | cherenkov |
T20449 | 599-602 | NN | denotes | cpm |
T20450 | 603-606 | VB | denotes | was |
T20451 | 607-611 | VB | denotes | used |
T20452 | 612-614 | TO | denotes | to |
T20453 | 615-624 | VB | denotes | hybridize |
T20454 | 625-627 | TO | denotes | to |
T20455 | 628-629 | CD | denotes | 4 |
T20456 | 630-632 | NN | denotes | μg |
T20457 | 633-635 | IN | denotes | of |
T20458 | 636-641 | JJ | denotes | total |
T20459 | 642-645 | NN | denotes | RNA |
T20460 | 646-651 | VB | denotes | using |
T20461 | 652-655 | DT | denotes | the |
T20462 | 656-659 | NN | denotes | RPA |
T20463 | 660-663 | NN | denotes | kit |
T20464 | 664-665 | -LRB- | denotes | ( |
T20465 | 665-667 | NN | denotes | BD |
T20466 | 668-679 | NNP | denotes | Biosciences |
T20467 | 679-680 | -RRB- | denotes | ) |
T20468 | 682-689 | NN | denotes | Samples |
T20469 | 690-694 | VB | denotes | were |
T20470 | 695-704 | VB | denotes | denatured |
T20471 | 705-707 | IN | denotes | at |
T20472 | 708-710 | CD | denotes | 90 |
T20473 | 711-713 | NN | denotes | °C |
T20474 | 714-717 | CC | denotes | and |
T20475 | 718-728 | VB | denotes | hybridized |
T20476 | 729-731 | IN | denotes | at |
T20477 | 732-734 | CD | denotes | 56 |
T20478 | 735-737 | NN | denotes | °C |
T20479 | 738-747 | RB | denotes | overnight |
T20480 | 749-764 | JJ | denotes | Single-stranded |
T20481 | 765-768 | NN | denotes | RNA |
T20482 | 769-772 | VB | denotes | was |
T20483 | 773-781 | VB | denotes | digested |
T20484 | 782-786 | IN | denotes | with |
T20485 | 787-788 | DT | denotes | a |
T20486 | 789-796 | NN | denotes | mixture |
T20487 | 797-799 | IN | denotes | of |
T20488 | 800-805 | NN | denotes | Rnase |
T20489 | 806-807 | NN | denotes | A |
T20490 | 808-811 | CC | denotes | and |
T20491 | 812-814 | NN | denotes | T1 |
T20492 | 814-815 | -COMMA- | denotes | , |
T20493 | 816-819 | CC | denotes | and |
T20494 | 820-832 | VB | denotes | precipitated |
T20495 | 833-838 | VB | denotes | using |
T20496 | 839-850 | NN | denotes | isopropanol |
T20497 | 852-861 | VB | denotes | Protected |
T20498 | 862-868 | NN | denotes | probes |
T20499 | 869-873 | VB | denotes | were |
T20500 | 874-882 | VB | denotes | resolved |
T20501 | 883-885 | IN | denotes | on |
T20502 | 886-887 | DT | denotes | a |
T20503 | 888-898 | VB | denotes | denaturing |
T20504 | 899-903 | CD | denotes | 4.75 |
T20505 | 903-904 | NN | denotes | % |
T20506 | 905-915 | NN | denotes | acrylamide |
T20507 | 916-919 | NN | denotes | gel |
T20508 | 919-920 | -COMMA- | denotes | , |
T20509 | 921-926 | VB | denotes | dried |
T20510 | 926-927 | -COMMA- | denotes | , |
T20511 | 928-931 | CC | denotes | and |
T20512 | 932-938 | VB | denotes | imaged |
T20513 | 939-944 | VB | denotes | using |
T20514 | 945-946 | DT | denotes | a |
T20515 | 947-961 | NN | denotes | phosphorImager |
T20516 | 962-963 | -LRB- | denotes | ( |
T20517 | 963-972 | NNP | denotes | Molecular |
T20518 | 973-981 | NNP | denotes | Dynamics |
T20519 | 981-982 | -RRB- | denotes | ) |
T20520 | 984-996 | NN | denotes | Densitometry |
T20521 | 997-1000 | VB | denotes | was |
T20522 | 1001-1010 | VB | denotes | performed |
T20523 | 1011-1016 | VB | denotes | using |
T20524 | 1017-1020 | DT | denotes | the |
T20525 | 1021-1031 | JJ | denotes | ImageQuant |
T20526 | 1032-1034 | NN | denotes | TL |
T20527 | 1035-1040 | NN | denotes | v2005 |
T20528 | 1041-1049 | NN | denotes | software |
T20529 | 1050-1051 | -LRB- | denotes | ( |
T20530 | 1051-1053 | NNP | denotes | GE |
T20531 | 1054-1064 | NNP | denotes | Healthcare |
T20532 | 1064-1065 | -RRB- | denotes | ) |
R15849 | T20346 | T20344 | arg1Of | assay,Rnase |
R15850 | T20346 | T20345 | arg1Of | assay,protection |
R15851 | T20348 | T20347 | arg1Of | RNA,Total |
R15852 | T20348 | T20349 | arg1Of | RNA,was |
R15853 | T20348 | T20350 | arg2Of | RNA,isolated |
R15854 | T20350 | T20349 | arg2Of | isolated,was |
R15855 | T20350 | T20351 | arg1Of | isolated,from |
R15856 | T20350 | T20365 | arg1Of | isolated,"," |
R15857 | T20350 | T20366 | arg1Of | isolated,according |
R15858 | T20355 | T20351 | arg2Of | cells,from |
R15859 | T20355 | T20352 | arg1Of | cells,CD19+ |
R15860 | T20355 | T20353 | arg1Of | cells,MACS-purified |
R15861 | T20355 | T20354 | arg1Of | cells,B |
R15862 | T20355 | T20356 | arg1Of | cells,using |
R15863 | T20361 | T20356 | arg2Of | kit,using |
R15864 | T20361 | T20357 | arg1Of | kit,the |
R15865 | T20361 | T20358 | arg1Of | kit,Rneasy |
R15866 | T20361 | T20359 | arg1Of | kit,midi |
R15867 | T20361 | T20360 | arg1Of | kit,purification |
R15868 | T20361 | T20362 | arg1Of | kit,with |
R15869 | T20364 | T20362 | arg2Of | treatment,with |
R15870 | T20364 | T20363 | arg1Of | treatment,Dnase |
R15871 | T20367 | T20366 | arg2Of | to,according |
R15872 | T20369 | T20368 | arg1Of | manufacturer,the |
R15873 | T20369 | T20370 | arg2Of | manufacturer,'s |
R15874 | T20371 | T20367 | arg2Of | instructions,to |
R15875 | T20371 | T20370 | arg1Of | instructions,'s |
R15876 | T20371 | T20372 | arg1Of | instructions,( |
R15877 | T20373 | T20372 | arg2Of | Qiagen,( |
R15878 | T20374 | T20372 | arg3Of | ),( |
R15879 | T20379 | T20375 | arg1Of | set,The |
R15880 | T20379 | T20376 | arg1Of | set,mCK-1 |
R15881 | T20379 | T20377 | arg1Of | set,probe |
R15882 | T20379 | T20378 | arg1Of | set,template |
R15883 | T20379 | T20380 | arg1Of | set,( |
R15884 | T20379 | T20384 | arg1Of | set,was |
R15885 | T20379 | T20385 | arg2Of | set,labeled |
R15886 | T20382 | T20380 | arg2Of | Biosciences,( |
R15887 | T20382 | T20381 | arg1Of | Biosciences,BD |
R15888 | T20383 | T20380 | arg3Of | ),( |
R15889 | T20385 | T20384 | arg2Of | labeled,was |
R15890 | T20385 | T20386 | modOf | labeled,using |
R15891 | T20386 | T20392 | arg1Of | using,according |
R15892 | T20389 | T20388 | arg1Of | Vitro,In |
R15893 | T20391 | T20386 | arg2Of | Kit,using |
R15894 | T20391 | T20387 | arg1Of | Kit,the |
R15895 | T20391 | T20389 | arg1Of | Kit,Vitro |
R15896 | T20391 | T20390 | arg1Of | Kit,Transcription |
R15897 | T20393 | T20392 | arg2Of | to,according |
R15898 | T20395 | T20394 | arg1Of | manufacturer,the |
R15899 | T20395 | T20396 | arg2Of | manufacturer,'s |
R15900 | T20397 | T20393 | arg2Of | instructions,to |
R15901 | T20397 | T20396 | arg1Of | instructions,'s |
R15902 | T20397 | T20398 | arg1Of | instructions,( |
R15903 | T20400 | T20398 | arg2Of | Biosciences,( |
R15904 | T20400 | T20399 | arg1Of | Biosciences,BD |
R15905 | T20401 | T20398 | arg3Of | ),( |
R15906 | T20405 | T20404 | arg1Of | ng,50 |
R15907 | T20405 | T20406 | arg1Of | ng,of |
R15908 | T20405 | T20410 | arg1Of | ng,was |
R15909 | T20405 | T20411 | arg2Of | ng,labeled |
R15910 | T20409 | T20406 | arg2Of | set,of |
R15911 | T20409 | T20407 | arg1Of | set,mCK-1 |
R15912 | T20409 | T20408 | arg1Of | set,probe |
R15913 | T20411 | T20402 | arg1Of | labeled,Briefly |
R15914 | T20411 | T20403 | arg1Of | labeled,"," |
R15915 | T20411 | T20410 | arg2Of | labeled,was |
R15916 | T20411 | T20412 | arg1Of | labeled,with |
R15917 | T20414 | T20413 | arg2Of | α-32P,[ |
R15918 | T20415 | T20413 | arg3Of | ],[ |
R15919 | T20416 | T20412 | arg2Of | UTP,with |
R15920 | T20416 | T20413 | arg1Of | UTP,[ |
R15921 | T20416 | T20417 | arg1Of | UTP,using |
R15922 | T20416 | T20422 | arg2Of | UTP,purified |
R15923 | T20417 | T20421 | arg1Of | using,and |
R15924 | T20420 | T20417 | arg2Of | polymerase,using |
R15925 | T20420 | T20418 | arg1Of | polymerase,T7 |
R15926 | T20420 | T20419 | arg1Of | polymerase,RNA |
R15927 | T20422 | T20421 | arg2Of | purified,and |
R15928 | T20422 | T20423 | modOf | purified,using |
R15929 | T20426 | T20423 | arg2Of | columns,using |
R15930 | T20426 | T20424 | arg1Of | columns,Sephadex |
R15931 | T20426 | T20425 | arg1Of | columns,G-50 |
R15932 | T20426 | T20427 | arg1Of | columns,( |
R15933 | T20429 | T20428 | arg1Of | Spin,NucAway |
R15934 | T20430 | T20427 | arg2Of | column,( |
R15935 | T20430 | T20429 | arg1Of | column,Spin |
R15936 | T20430 | T20431 | arg1Of | column,"," |
R15937 | T20432 | T20431 | arg2Of | Ambion,"," |
R15938 | T20433 | T20427 | arg3Of | ),( |
R15939 | T20436 | T20434 | arg1Of | probe,The |
R15940 | T20436 | T20435 | arg2Of | probe,labeled |
R15941 | T20436 | T20437 | arg1Of | probe,was |
R15942 | T20436 | T20438 | arg2Of | probe,quantitated |
R15943 | T20436 | T20439 | arg1Of | probe,using |
R15944 | T20438 | T20437 | arg2Of | quantitated,was |
R15945 | T20438 | T20444 | arg1Of | quantitated,and |
R15946 | T20439 | T20438 | arg3Of | using,quantitated |
R15947 | T20442 | T20439 | arg2Of | counter,using |
R15948 | T20442 | T20440 | arg1Of | counter,a |
R15949 | T20442 | T20441 | arg1Of | counter,scintillation |
R15950 | T20444 | T20443 | arg1Of | and,"," |
R15951 | T20447 | T20446 | arg1Of | 105,× |
R15952 | T20449 | T20445 | arg1Of | cpm,6 |
R15953 | T20449 | T20447 | arg1Of | cpm,105 |
R15954 | T20449 | T20448 | arg1Of | cpm,cherenkov |
R15955 | T20449 | T20450 | arg1Of | cpm,was |
R15956 | T20449 | T20451 | arg2Of | cpm,used |
R15957 | T20449 | T20453 | arg1Of | cpm,hybridize |
R15958 | T20451 | T20444 | arg2Of | used,and |
R15959 | T20451 | T20450 | arg2Of | used,was |
R15960 | T20453 | T20451 | arg3Of | hybridize,used |
R15961 | T20453 | T20452 | arg1Of | hybridize,to |
R15962 | T20453 | T20454 | arg1Of | hybridize,to |
R15963 | T20456 | T20454 | arg2Of | μg,to |
R15964 | T20456 | T20455 | arg1Of | μg,4 |
R15965 | T20456 | T20457 | arg1Of | μg,of |
R15966 | T20459 | T20457 | arg2Of | RNA,of |
R15967 | T20459 | T20458 | arg1Of | RNA,total |
R15968 | T20459 | T20460 | arg1Of | RNA,using |
R15969 | T20463 | T20460 | arg2Of | kit,using |
R15970 | T20463 | T20461 | arg1Of | kit,the |
R15971 | T20463 | T20462 | arg1Of | kit,RPA |
R15972 | T20463 | T20464 | arg1Of | kit,( |
R15973 | T20466 | T20464 | arg2Of | Biosciences,( |
R15974 | T20466 | T20465 | arg1Of | Biosciences,BD |
R15975 | T20467 | T20464 | arg3Of | ),( |
R15976 | T20468 | T20469 | arg1Of | Samples,were |
R15977 | T20468 | T20470 | arg2Of | Samples,denatured |
R15978 | T20468 | T20475 | arg1Of | Samples,hybridized |
R15979 | T20470 | T20469 | arg2Of | denatured,were |
R15980 | T20470 | T20471 | arg1Of | denatured,at |
R15981 | T20470 | T20474 | arg1Of | denatured,and |
R15982 | T20473 | T20471 | arg2Of | °C,at |
R15983 | T20473 | T20472 | arg1Of | °C,90 |
R15984 | T20475 | T20474 | arg2Of | hybridized,and |
R15985 | T20475 | T20476 | arg1Of | hybridized,at |
R15986 | T20478 | T20476 | arg2Of | °C,at |
R15987 | T20478 | T20477 | arg1Of | °C,56 |
R15988 | T20478 | T20479 | arg1Of | °C,overnight |
R15989 | T20481 | T20480 | arg1Of | RNA,Single-stranded |
R15990 | T20481 | T20482 | arg1Of | RNA,was |
R15991 | T20481 | T20483 | arg2Of | RNA,digested |
R15992 | T20481 | T20494 | arg2Of | RNA,precipitated |
R15993 | T20481 | T20495 | arg1Of | RNA,using |
R15994 | T20483 | T20484 | arg1Of | digested,with |
R15995 | T20483 | T20493 | arg1Of | digested,and |
R15996 | T20486 | T20484 | arg2Of | mixture,with |
R15997 | T20486 | T20485 | arg1Of | mixture,a |
R15998 | T20486 | T20487 | arg1Of | mixture,of |
R15999 | T20489 | T20488 | arg1Of | A,Rnase |
R16000 | T20489 | T20490 | arg1Of | A,and |
R16001 | T20490 | T20487 | arg2Of | and,of |
R16002 | T20491 | T20490 | arg2Of | T1,and |
R16003 | T20493 | T20482 | arg2Of | and,was |
R16004 | T20493 | T20492 | arg1Of | and,"," |
R16005 | T20494 | T20493 | arg2Of | precipitated,and |
R16006 | T20495 | T20494 | arg3Of | using,precipitated |
R16007 | T20496 | T20495 | arg2Of | isopropanol,using |
R16008 | T20498 | T20497 | arg2Of | probes,Protected |
R16009 | T20498 | T20499 | arg1Of | probes,were |
R16010 | T20498 | T20500 | arg2Of | probes,resolved |
R16011 | T20498 | T20512 | arg2Of | probes,imaged |
R16012 | T20498 | T20513 | arg1Of | probes,using |
R16013 | T20500 | T20501 | arg1Of | resolved,on |
R16014 | T20500 | T20511 | arg1Of | resolved,and |
R16015 | T20504 | T20505 | arg1Of | 4.75,% |
R16016 | T20507 | T20501 | arg2Of | gel,on |
R16017 | T20507 | T20502 | arg1Of | gel,a |
R16018 | T20507 | T20503 | arg1Of | gel,denaturing |
R16019 | T20507 | T20504 | arg1Of | gel,4.75 |
R16020 | T20507 | T20506 | arg1Of | gel,acrylamide |
R16021 | T20507 | T20508 | arg1Of | gel,"," |
R16022 | T20507 | T20509 | arg2Of | gel,dried |
R16023 | T20511 | T20499 | arg2Of | and,were |
R16024 | T20511 | T20510 | arg1Of | and,"," |
R16025 | T20512 | T20511 | arg2Of | imaged,and |
R16026 | T20513 | T20512 | arg3Of | using,imaged |
R16027 | T20515 | T20513 | arg2Of | phosphorImager,using |
R16028 | T20515 | T20514 | arg1Of | phosphorImager,a |
R16029 | T20515 | T20516 | arg1Of | phosphorImager,( |
R16030 | T20518 | T20516 | arg2Of | Dynamics,( |
R16031 | T20518 | T20517 | arg1Of | Dynamics,Molecular |
R16032 | T20519 | T20516 | arg3Of | ),( |
R16033 | T20520 | T20521 | arg1Of | Densitometry,was |
R16034 | T20520 | T20522 | arg2Of | Densitometry,performed |
R16035 | T20522 | T20521 | arg2Of | performed,was |
R16036 | T20523 | T20522 | arg3Of | using,performed |
R16037 | T20528 | T20523 | arg2Of | software,using |
R16038 | T20528 | T20524 | arg1Of | software,the |
R16039 | T20528 | T20525 | arg1Of | software,ImageQuant |
R16040 | T20528 | T20526 | arg1Of | software,TL |
R16041 | T20528 | T20527 | arg1Of | software,v2005 |
R16042 | T20528 | T20529 | arg1Of | software,( |
R16043 | T20531 | T20529 | arg2Of | Healthcare,( |
R16044 | T20531 | T20530 | arg1Of | Healthcare,GE |
R16045 | T20532 | T20529 | arg3Of | ),( |
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20534 | 0-5 | JJ | denotes | Rnase |
T20535 | 6-16 | NN | denotes | protection |
T20536 | 17-22 | NN | denotes | assay |
T20537 | 22-23 | . | denotes | . |
T20538 | 24-29 | JJ | denotes | Total |
T20539 | 30-33 | NNP | denotes | RNA |
T20540 | 34-37 | VBD | denotes | was |
T20541 | 38-46 | VBN | denotes | isolated |
T20542 | 47-51 | IN | denotes | from |
T20543 | 52-56 | CD | denotes | CD19 |
T20544 | 56-57 | CD | denotes | + |
T20545 | 58-71 | JJ | denotes | MACS-purified |
T20546 | 72-73 | NNP | denotes | B |
T20547 | 74-79 | NNS | denotes | cells |
T20548 | 80-85 | VBG | denotes | using |
T20549 | 86-89 | DT | denotes | the |
T20550 | 90-96 | NNP | denotes | Rneasy |
T20551 | 97-101 | NN | denotes | midi |
T20552 | 102-114 | NN | denotes | purification |
T20553 | 115-118 | NN | denotes | kit |
T20554 | 119-123 | IN | denotes | with |
T20555 | 124-129 | NNP | denotes | Dnase |
T20556 | 130-139 | NN | denotes | treatment |
T20557 | 139-140 | , | denotes | , |
T20558 | 141-150 | VBG | denotes | according |
T20559 | 151-153 | TO | denotes | to |
T20560 | 154-157 | DT | denotes | the |
T20561 | 158-170 | NN | denotes | manufacturer |
T20562 | 170-172 | POS | denotes | 's |
T20563 | 173-185 | NNS | denotes | instructions |
T20564 | 186-187 | -LRB- | denotes | ( |
T20565 | 187-193 | NNP | denotes | Qiagen |
T20566 | 193-194 | -RRB- | denotes | ) |
T20567 | 194-195 | . | denotes | . |
T20568 | 196-199 | DT | denotes | The |
T20569 | 200-205 | JJ | denotes | mCK-1 |
T20570 | 206-211 | NN | denotes | probe |
T20571 | 212-220 | NN | denotes | template |
T20572 | 221-224 | NN | denotes | set |
T20573 | 225-226 | -LRB- | denotes | ( |
T20574 | 226-228 | NNP | denotes | BD |
T20575 | 229-240 | NNPS | denotes | Biosciences |
T20576 | 240-241 | -RRB- | denotes | ) |
T20577 | 242-245 | VBD | denotes | was |
T20578 | 246-253 | VBN | denotes | labeled |
T20579 | 254-259 | VBG | denotes | using |
T20580 | 260-263 | DT | denotes | the |
T20581 | 264-266 | NNP | denotes | In |
T20582 | 267-272 | NNP | denotes | Vitro |
T20583 | 273-286 | NNP | denotes | Transcription |
T20584 | 287-290 | NNP | denotes | Kit |
T20585 | 291-300 | VBG | denotes | according |
T20586 | 301-303 | TO | denotes | to |
T20587 | 304-307 | DT | denotes | the |
T20588 | 308-320 | NN | denotes | manufacturer |
T20589 | 320-322 | POS | denotes | 's |
T20590 | 323-335 | NNS | denotes | instructions |
T20591 | 336-337 | -LRB- | denotes | ( |
T20592 | 337-339 | NNP | denotes | BD |
T20593 | 340-351 | NNPS | denotes | Biosciences |
T20594 | 351-352 | -RRB- | denotes | ) |
T20595 | 352-353 | . | denotes | . |
T20596 | 354-361 | RB | denotes | Briefly |
T20597 | 361-362 | , | denotes | , |
T20598 | 363-365 | CD | denotes | 50 |
T20599 | 366-368 | NN | denotes | ng |
T20600 | 369-371 | IN | denotes | of |
T20601 | 372-377 | JJ | denotes | mCK-1 |
T20602 | 378-383 | NN | denotes | probe |
T20603 | 384-387 | NN | denotes | set |
T20604 | 388-391 | VBD | denotes | was |
T20605 | 392-399 | VBN | denotes | labeled |
T20606 | 400-404 | IN | denotes | with |
T20607 | 405-406 | NNP | denotes | [ |
T20608 | 406-411 | NNP | denotes | α-32P |
T20609 | 411-412 | NNP | denotes | ] |
T20610 | 412-415 | NNP | denotes | UTP |
T20611 | 416-421 | VBG | denotes | using |
T20612 | 422-424 | CD | denotes | T7 |
T20613 | 425-428 | NNP | denotes | RNA |
T20614 | 429-439 | NN | denotes | polymerase |
T20615 | 440-443 | CC | denotes | and |
T20616 | 444-452 | VBD | denotes | purified |
T20617 | 453-458 | VBG | denotes | using |
T20618 | 459-467 | NNP | denotes | Sephadex |
T20619 | 468-472 | NNP | denotes | G-50 |
T20620 | 473-480 | NNS | denotes | columns |
T20621 | 481-482 | -LRB- | denotes | ( |
T20622 | 482-489 | NNP | denotes | NucAway |
T20623 | 490-494 | NNP | denotes | Spin |
T20624 | 495-501 | NN | denotes | column |
T20625 | 501-502 | , | denotes | , |
T20626 | 503-509 | NNP | denotes | Ambion |
T20627 | 509-510 | -RRB- | denotes | ) |
T20628 | 510-511 | . | denotes | . |
T20629 | 512-515 | DT | denotes | The |
T20630 | 516-523 | VBN | denotes | labeled |
T20631 | 524-529 | NN | denotes | probe |
T20632 | 530-533 | VBD | denotes | was |
T20633 | 534-545 | VBN | denotes | quantitated |
T20634 | 546-551 | VBG | denotes | using |
T20635 | 552-553 | DT | denotes | a |
T20636 | 554-567 | NN | denotes | scintillation |
T20637 | 568-575 | RB | denotes | counter |
T20638 | 575-576 | , | denotes | , |
T20639 | 577-580 | CC | denotes | and |
T20640 | 581-582 | CD | denotes | 6 |
T20641 | 583-584 | NN | denotes | × |
T20642 | 585-588 | CD | denotes | 105 |
T20643 | 589-598 | NN | denotes | cherenkov |
T20644 | 599-602 | NN | denotes | cpm |
T20645 | 603-606 | VBD | denotes | was |
T20646 | 607-611 | VBN | denotes | used |
T20647 | 612-614 | TO | denotes | to |
T20648 | 615-624 | VB | denotes | hybridize |
T20649 | 625-627 | TO | denotes | to |
T20650 | 628-629 | CD | denotes | 4 |
T20651 | 630-632 | NN | denotes | μg |
T20652 | 633-635 | IN | denotes | of |
T20653 | 636-641 | JJ | denotes | total |
T20654 | 642-645 | NNP | denotes | RNA |
T20655 | 646-651 | VBG | denotes | using |
T20656 | 652-655 | DT | denotes | the |
T20657 | 656-659 | NNP | denotes | RPA |
T20658 | 660-663 | NN | denotes | kit |
T20659 | 664-665 | -LRB- | denotes | ( |
T20660 | 665-667 | NNP | denotes | BD |
T20661 | 668-679 | NNPS | denotes | Biosciences |
T20662 | 679-680 | -RRB- | denotes | ) |
T20663 | 680-681 | . | denotes | . |
T20664 | 682-689 | NNS | denotes | Samples |
T20665 | 690-694 | VBD | denotes | were |
T20666 | 695-704 | VBN | denotes | denatured |
T20667 | 705-707 | IN | denotes | at |
T20668 | 708-710 | CD | denotes | 90 |
T20669 | 711-712 | CD | denotes | ° |
T20670 | 712-713 | NNP | denotes | C |
T20671 | 714-717 | CC | denotes | and |
T20672 | 718-728 | VBN | denotes | hybridized |
T20673 | 729-731 | IN | denotes | at |
T20674 | 732-734 | CD | denotes | 56 |
T20675 | 735-736 | CD | denotes | ° |
T20676 | 736-737 | NNP | denotes | C |
T20677 | 738-747 | JJ | denotes | overnight |
T20678 | 747-748 | . | denotes | . |
T20679 | 749-764 | JJ | denotes | Single-stranded |
T20680 | 765-768 | NNP | denotes | RNA |
T20681 | 769-772 | VBD | denotes | was |
T20682 | 773-781 | VBN | denotes | digested |
T20683 | 782-786 | IN | denotes | with |
T20684 | 787-788 | DT | denotes | a |
T20685 | 789-796 | NN | denotes | mixture |
T20686 | 797-799 | IN | denotes | of |
T20687 | 800-805 | NNP | denotes | Rnase |
T20688 | 806-807 | NNP | denotes | A |
T20689 | 808-811 | CC | denotes | and |
T20690 | 812-814 | NNP | denotes | T1 |
T20691 | 814-815 | , | denotes | , |
T20692 | 816-819 | CC | denotes | and |
T20693 | 820-832 | VBD | denotes | precipitated |
T20694 | 833-838 | VBG | denotes | using |
T20695 | 839-850 | NN | denotes | isopropanol |
T20696 | 850-851 | . | denotes | . |
T20697 | 852-861 | VBN | denotes | Protected |
T20698 | 862-868 | NNS | denotes | probes |
T20699 | 869-873 | VBD | denotes | were |
T20700 | 874-882 | VBN | denotes | resolved |
T20701 | 883-885 | IN | denotes | on |
T20702 | 886-887 | DT | denotes | a |
T20703 | 888-898 | VBG | denotes | denaturing |
T20704 | 899-903 | CD | denotes | 4.75 |
T20705 | 903-904 | NN | denotes | % |
T20706 | 905-915 | NN | denotes | acrylamide |
T20707 | 916-919 | NN | denotes | gel |
T20708 | 919-920 | , | denotes | , |
T20709 | 921-926 | VBD | denotes | dried |
T20710 | 926-927 | , | denotes | , |
T20711 | 928-931 | CC | denotes | and |
T20712 | 932-938 | VBD | denotes | imaged |
T20713 | 939-944 | VBG | denotes | using |
T20714 | 945-946 | DT | denotes | a |
T20715 | 947-961 | NNP | denotes | phosphorImager |
T20716 | 962-963 | -LRB- | denotes | ( |
T20717 | 963-972 | NNP | denotes | Molecular |
T20718 | 973-981 | NNP | denotes | Dynamics |
T20719 | 981-982 | -RRB- | denotes | ) |
T20720 | 982-983 | . | denotes | . |
T20721 | 984-996 | NNP | denotes | Densitometry |
T20722 | 997-1000 | VBD | denotes | was |
T20723 | 1001-1010 | VBN | denotes | performed |
T20724 | 1011-1016 | VBG | denotes | using |
T20725 | 1017-1020 | DT | denotes | the |
T20726 | 1021-1031 | NNP | denotes | ImageQuant |
T20727 | 1032-1034 | NNP | denotes | TL |
T20728 | 1035-1040 | CD | denotes | v2005 |
T20729 | 1041-1049 | NN | denotes | software |
T20730 | 1050-1051 | -LRB- | denotes | ( |
T20731 | 1051-1053 | NNP | denotes | GE |
T20732 | 1054-1064 | NNP | denotes | Healthcare |
T20733 | 1064-1065 | -RRB- | denotes | ) |
T20734 | 1065-1066 | . | denotes | . |
R16046 | T20534 | T20536 | amod | Rnase,assay |
R16047 | T20535 | T20536 | compound | protection,assay |
R16048 | T20536 | T20536 | ROOT | assay,assay |
R16049 | T20537 | T20536 | punct | .,assay |
R16050 | T20538 | T20539 | amod | Total,RNA |
R16051 | T20539 | T20541 | nsubjpass | RNA,isolated |
R16052 | T20540 | T20541 | auxpass | was,isolated |
R16053 | T20541 | T20541 | ROOT | isolated,isolated |
R16054 | T20542 | T20541 | prep | from,isolated |
R16055 | T20543 | T20547 | nummod | CD19,cells |
R16056 | T20544 | T20547 | nummod | +,cells |
R16057 | T20545 | T20547 | amod | MACS-purified,cells |
R16058 | T20546 | T20547 | compound | B,cells |
R16059 | T20547 | T20542 | pobj | cells,from |
R16060 | T20548 | T20541 | advcl | using,isolated |
R16061 | T20549 | T20553 | det | the,kit |
R16062 | T20550 | T20553 | nmod | Rneasy,kit |
R16063 | T20551 | T20553 | amod | midi,kit |
R16064 | T20552 | T20553 | compound | purification,kit |
R16065 | T20553 | T20548 | dobj | kit,using |
R16066 | T20554 | T20553 | prep | with,kit |
R16067 | T20555 | T20556 | compound | Dnase,treatment |
R16068 | T20556 | T20554 | pobj | treatment,with |
R16069 | T20557 | T20541 | punct | ",",isolated |
R16070 | T20558 | T20541 | prep | according,isolated |
R16071 | T20559 | T20558 | prep | to,according |
R16072 | T20560 | T20561 | det | the,manufacturer |
R16073 | T20561 | T20563 | poss | manufacturer,instructions |
R16074 | T20562 | T20561 | case | 's,manufacturer |
R16075 | T20563 | T20559 | pobj | instructions,to |
R16076 | T20564 | T20565 | punct | (,Qiagen |
R16077 | T20565 | T20563 | appos | Qiagen,instructions |
R16078 | T20566 | T20563 | punct | ),instructions |
R16079 | T20567 | T20541 | punct | .,isolated |
R16080 | T20568 | T20571 | det | The,template |
R16081 | T20569 | T20571 | amod | mCK-1,template |
R16082 | T20570 | T20571 | compound | probe,template |
R16083 | T20571 | T20578 | nsubjpass | template,labeled |
R16084 | T20572 | T20571 | acl | set,template |
R16085 | T20573 | T20575 | punct | (,Biosciences |
R16086 | T20574 | T20575 | compound | BD,Biosciences |
R16087 | T20575 | T20572 | appos | Biosciences,set |
R16088 | T20576 | T20572 | punct | ),set |
R16089 | T20577 | T20578 | auxpass | was,labeled |
R16090 | T20578 | T20578 | ROOT | labeled,labeled |
R16091 | T20579 | T20578 | advcl | using,labeled |
R16092 | T20580 | T20584 | det | the,Kit |
R16093 | T20581 | T20584 | compound | In,Kit |
R16094 | T20582 | T20583 | compound | Vitro,Transcription |
R16095 | T20583 | T20584 | compound | Transcription,Kit |
R16096 | T20584 | T20579 | dobj | Kit,using |
R16097 | T20585 | T20578 | prep | according,labeled |
R16098 | T20586 | T20585 | prep | to,according |
R16099 | T20587 | T20588 | det | the,manufacturer |
R16100 | T20588 | T20590 | poss | manufacturer,instructions |
R16101 | T20589 | T20588 | case | 's,manufacturer |
R16102 | T20590 | T20586 | pobj | instructions,to |
R16103 | T20591 | T20593 | punct | (,Biosciences |
R16104 | T20592 | T20593 | compound | BD,Biosciences |
R16105 | T20593 | T20590 | appos | Biosciences,instructions |
R16106 | T20594 | T20590 | punct | ),instructions |
R16107 | T20595 | T20578 | punct | .,labeled |
R16108 | T20596 | T20605 | advmod | Briefly,labeled |
R16109 | T20597 | T20605 | punct | ",",labeled |
R16110 | T20598 | T20599 | nummod | 50,ng |
R16111 | T20599 | T20605 | nsubjpass | ng,labeled |
R16112 | T20600 | T20599 | prep | of,ng |
R16113 | T20601 | T20603 | amod | mCK-1,set |
R16114 | T20602 | T20603 | compound | probe,set |
R16115 | T20603 | T20600 | pobj | set,of |
R16116 | T20604 | T20605 | auxpass | was,labeled |
R16117 | T20605 | T20605 | ROOT | labeled,labeled |
R16118 | T20606 | T20605 | prep | with,labeled |
R16119 | T20607 | T20610 | compound | [,UTP |
R16120 | T20608 | T20610 | compound | α-32P,UTP |
R16121 | T20609 | T20610 | compound | ],UTP |
R16122 | T20610 | T20606 | pobj | UTP,with |
R16123 | T20611 | T20605 | advcl | using,labeled |
R16124 | T20612 | T20614 | nummod | T7,polymerase |
R16125 | T20613 | T20614 | compound | RNA,polymerase |
R16126 | T20614 | T20611 | dobj | polymerase,using |
R16127 | T20615 | T20605 | cc | and,labeled |
R16128 | T20616 | T20605 | conj | purified,labeled |
R16129 | T20617 | T20616 | xcomp | using,purified |
R16130 | T20618 | T20619 | compound | Sephadex,G-50 |
R16131 | T20619 | T20620 | compound | G-50,columns |
R16132 | T20620 | T20617 | dobj | columns,using |
R16133 | T20621 | T20620 | punct | (,columns |
R16134 | T20622 | T20623 | compound | NucAway,Spin |
R16135 | T20623 | T20624 | compound | Spin,column |
R16136 | T20624 | T20620 | appos | column,columns |
R16137 | T20625 | T20624 | punct | ",",column |
R16138 | T20626 | T20624 | appos | Ambion,column |
R16139 | T20627 | T20624 | punct | ),column |
R16140 | T20628 | T20605 | punct | .,labeled |
R16141 | T20629 | T20631 | det | The,probe |
R16142 | T20630 | T20631 | amod | labeled,probe |
R16143 | T20631 | T20633 | nsubjpass | probe,quantitated |
R16144 | T20632 | T20633 | auxpass | was,quantitated |
R16145 | T20633 | T20633 | ROOT | quantitated,quantitated |
R16146 | T20634 | T20633 | advcl | using,quantitated |
R16147 | T20635 | T20636 | det | a,scintillation |
R16148 | T20636 | T20634 | dobj | scintillation,using |
R16149 | T20637 | T20634 | advmod | counter,using |
R16150 | T20638 | T20634 | punct | ",",using |
R16151 | T20639 | T20633 | cc | and,quantitated |
R16152 | T20640 | T20644 | nummod | 6,cpm |
R16153 | T20641 | T20644 | nmod | ×,cpm |
R16154 | T20642 | T20641 | nummod | 105,× |
R16155 | T20643 | T20644 | compound | cherenkov,cpm |
R16156 | T20644 | T20646 | nsubjpass | cpm,used |
R16157 | T20645 | T20646 | auxpass | was,used |
R16158 | T20646 | T20633 | conj | used,quantitated |
R16159 | T20647 | T20648 | aux | to,hybridize |
R16160 | T20648 | T20646 | xcomp | hybridize,used |
R16161 | T20649 | T20648 | prep | to,hybridize |
R16162 | T20650 | T20651 | nummod | 4,μg |
R16163 | T20651 | T20649 | pobj | μg,to |
R16164 | T20652 | T20651 | prep | of,μg |
R16165 | T20653 | T20654 | amod | total,RNA |
R16166 | T20654 | T20652 | pobj | RNA,of |
R16167 | T20655 | T20648 | advcl | using,hybridize |
R16168 | T20656 | T20658 | det | the,kit |
R16169 | T20657 | T20658 | compound | RPA,kit |
R16170 | T20658 | T20655 | dobj | kit,using |
R16171 | T20659 | T20658 | punct | (,kit |
R16172 | T20660 | T20661 | compound | BD,Biosciences |
R16173 | T20661 | T20658 | appos | Biosciences,kit |
R16174 | T20662 | T20658 | punct | ),kit |
R16175 | T20663 | T20646 | punct | .,used |
R16176 | T20664 | T20666 | nsubjpass | Samples,denatured |
R16177 | T20665 | T20666 | auxpass | were,denatured |
R16178 | T20666 | T20666 | ROOT | denatured,denatured |
R16179 | T20667 | T20666 | prep | at,denatured |
R16180 | T20668 | T20669 | nummod | 90,° |
R16181 | T20669 | T20667 | pobj | °,at |
R16182 | T20670 | T20666 | conj | C,denatured |
R16183 | T20671 | T20670 | cc | and,C |
R16184 | T20672 | T20670 | conj | hybridized,C |
R16185 | T20673 | T20672 | prep | at,hybridized |
R16186 | T20674 | T20676 | nummod | 56,C |
R16187 | T20675 | T20676 | nummod | °,C |
R16188 | T20676 | T20677 | compound | C,overnight |
R16189 | T20677 | T20673 | pobj | overnight,at |
R16190 | T20678 | T20666 | punct | .,denatured |
R16191 | T20679 | T20680 | compound | Single-stranded,RNA |
R16192 | T20680 | T20682 | nsubjpass | RNA,digested |
R16193 | T20681 | T20682 | auxpass | was,digested |
R16194 | T20682 | T20682 | ROOT | digested,digested |
R16195 | T20683 | T20682 | prep | with,digested |
R16196 | T20684 | T20685 | det | a,mixture |
R16197 | T20685 | T20683 | pobj | mixture,with |
R16198 | T20686 | T20685 | prep | of,mixture |
R16199 | T20687 | T20688 | compound | Rnase,A |
R16200 | T20688 | T20686 | pobj | A,of |
R16201 | T20689 | T20688 | cc | and,A |
R16202 | T20690 | T20688 | conj | T1,A |
R16203 | T20691 | T20690 | punct | ",",T1 |
R16204 | T20692 | T20682 | cc | and,digested |
R16205 | T20693 | T20682 | conj | precipitated,digested |
R16206 | T20694 | T20693 | xcomp | using,precipitated |
R16207 | T20695 | T20694 | dobj | isopropanol,using |
R16208 | T20696 | T20682 | punct | .,digested |
R16209 | T20697 | T20698 | amod | Protected,probes |
R16210 | T20698 | T20700 | nsubjpass | probes,resolved |
R16211 | T20699 | T20700 | auxpass | were,resolved |
R16212 | T20700 | T20700 | ROOT | resolved,resolved |
R16213 | T20701 | T20700 | prep | on,resolved |
R16214 | T20702 | T20707 | det | a,gel |
R16215 | T20703 | T20707 | amod | denaturing,gel |
R16216 | T20704 | T20705 | nummod | 4.75,% |
R16217 | T20705 | T20707 | compound | %,gel |
R16218 | T20706 | T20707 | compound | acrylamide,gel |
R16219 | T20707 | T20701 | pobj | gel,on |
R16220 | T20708 | T20709 | punct | ",",dried |
R16221 | T20709 | T20700 | conj | dried,resolved |
R16222 | T20710 | T20709 | punct | ",",dried |
R16223 | T20711 | T20709 | cc | and,dried |
R16224 | T20712 | T20709 | conj | imaged,dried |
R16225 | T20713 | T20712 | xcomp | using,imaged |
R16226 | T20714 | T20715 | det | a,phosphorImager |
R16227 | T20715 | T20713 | dobj | phosphorImager,using |
R16228 | T20716 | T20718 | punct | (,Dynamics |
R16229 | T20717 | T20718 | compound | Molecular,Dynamics |
R16230 | T20718 | T20715 | appos | Dynamics,phosphorImager |
R16231 | T20719 | T20715 | punct | ),phosphorImager |
R16232 | T20720 | T20709 | punct | .,dried |
R16233 | T20721 | T20723 | nsubjpass | Densitometry,performed |
R16234 | T20722 | T20723 | auxpass | was,performed |
R16235 | T20723 | T20723 | ROOT | performed,performed |
R16236 | T20724 | T20723 | advcl | using,performed |
R16237 | T20725 | T20729 | det | the,software |
R16238 | T20726 | T20729 | nmod | ImageQuant,software |
R16239 | T20727 | T20729 | nmod | TL,software |
R16240 | T20728 | T20729 | nummod | v2005,software |
R16241 | T20729 | T20724 | dobj | software,using |
R16242 | T20730 | T20729 | punct | (,software |
R16243 | T20731 | T20732 | compound | GE,Healthcare |
R16244 | T20732 | T20729 | appos | Healthcare,software |
R16245 | T20733 | T20729 | punct | ),software |
R16246 | T20734 | T20723 | punct | .,performed |
GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20343 | 273-286 | http://purl.obolibrary.org/obo/GO_0006351 | denotes | Transcription |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20744 | 74-79 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T20745 | 656-659 | http://purl.obolibrary.org/obo/GO_0005662 | denotes | RPA |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20334 | 0-23 | Sentence | denotes | Rnase protection assay. |
T20335 | 24-195 | Sentence | denotes | Total RNA was isolated from CD19+ MACS-purified B cells using the Rneasy midi purification kit with Dnase treatment, according to the manufacturer's instructions (Qiagen). |
T20336 | 196-353 | Sentence | denotes | The mCK-1 probe template set (BD Biosciences) was labeled using the In Vitro Transcription Kit according to the manufacturer's instructions (BD Biosciences). |
T20337 | 354-511 | Sentence | denotes | Briefly, 50 ng of mCK-1 probe set was labeled with [α-32P]UTP using T7 RNA polymerase and purified using Sephadex G-50 columns (NucAway Spin column, Ambion). |
T20338 | 512-681 | Sentence | denotes | The labeled probe was quantitated using a scintillation counter, and 6 × 105 cherenkov cpm was used to hybridize to 4 μg of total RNA using the RPA kit (BD Biosciences). |
T20339 | 682-748 | Sentence | denotes | Samples were denatured at 90 °C and hybridized at 56 °C overnight. |
T20340 | 749-851 | Sentence | denotes | Single-stranded RNA was digested with a mixture of Rnase A and T1, and precipitated using isopropanol. |
T20341 | 852-983 | Sentence | denotes | Protected probes were resolved on a denaturing 4.75% acrylamide gel, dried, and imaged using a phosphorImager (Molecular Dynamics). |
T20342 | 984-1066 | Sentence | denotes | Densitometry was performed using the ImageQuant TL v2005 software (GE Healthcare). |
T327 | 0-23 | Sentence | denotes | Rnase protection assay. |
T328 | 24-195 | Sentence | denotes | Total RNA was isolated from CD19+ MACS-purified B cells using the Rneasy midi purification kit with Dnase treatment, according to the manufacturer's instructions (Qiagen). |
T329 | 196-353 | Sentence | denotes | The mCK-1 probe template set (BD Biosciences) was labeled using the In Vitro Transcription Kit according to the manufacturer's instructions (BD Biosciences). |
T330 | 354-511 | Sentence | denotes | Briefly, 50 ng of mCK-1 probe set was labeled with [α-32P]UTP using T7 RNA polymerase and purified using Sephadex G-50 columns (NucAway Spin column, Ambion). |
T331 | 512-681 | Sentence | denotes | The labeled probe was quantitated using a scintillation counter, and 6 × 105 cherenkov cpm was used to hybridize to 4 μg of total RNA using the RPA kit (BD Biosciences). |
T332 | 682-748 | Sentence | denotes | Samples were denatured at 90 °C and hybridized at 56 °C overnight. |
T333 | 749-851 | Sentence | denotes | Single-stranded RNA was digested with a mixture of Rnase A and T1, and precipitated using isopropanol. |
T334 | 852-983 | Sentence | denotes | Protected probes were resolved on a denaturing 4.75% acrylamide gel, dried, and imaged using a phosphorImager (Molecular Dynamics). |
T335 | 984-1066 | Sentence | denotes | Densitometry was performed using the ImageQuant TL v2005 software (GE Healthcare). |
events-check-again
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20761 | 52-56 | Protein | denotes | CD19 |
T20762 | 800-807 | Protein | denotes | Rnase A |
T20763 | 812-814 | Protein | denotes | T1 |
bionlp-st-ge-2016-reference-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20752 | 0-5 | Protein | denotes | Rnase |
T20753 | 52-56 | Protein | denotes | CD19 |
T20754 | 58-62 | Protein | denotes | MACS |
T20755 | 124-129 | Protein | denotes | Dnase |
T20756 | 200-205 | Protein | denotes | mCK-1 |
T20757 | 372-387 | Protein | denotes | mCK-1 probe set |
T20758 | 422-439 | Protein | denotes | T7 RNA polymerase |
T20759 | 490-494 | Protein | denotes | Spin |
T20760 | 800-805 | Protein | denotes | Rnase |
bionlp-st-ge-2016-reference
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20331 | 52-56 | Protein | denotes | CD19 |
T20332 | 800-807 | Protein | denotes | Rnase A |
T20333 | 812-814 | Protein | denotes | T1 |
bionlp-st-ge-2016-uniprot
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20533 | 52-56 | P15391 | denotes | CD19 |
test2
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T20328 | 52-56 | Protein | denotes | CD19 |
T20329 | 800-807 | Protein | denotes | Rnase A |
T20330 | 812-814 | Protein | denotes | T1 |