PMC:2065877 / 42522-51191 JSON TXT

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2_test

Id	Subject	Object	Predicate	Lexical cue
17997602-9751773-98185781	202-204	9751773	denotes	26
17997602-9751773-98185782	303-305	9751773	denotes	26
17997602-8027546-98185783	6449-6451	8027546	denotes	74

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T18295	23-33	JJ	denotes	Transgenic
T18296	34-38	NN	denotes	mice
T18297	40-50	NN	denotes	Generation
T18298	51-53	IN	denotes	of
T18299	54-58	NN	denotes	LMP1
T18300	59-63	NN	denotes	mice
T18301	64-69	IN	denotes	under
T18302	70-73	DT	denotes	the
T18303	74-76	NN	denotes	Ig
T18304	77-82	JJ	denotes	heavy
T18305	83-88	NN	denotes	chain
T18306	89-97	NN	denotes	promoter
T18307	98-101	CC	denotes	and
T18308	102-110	NN	denotes	enhancer
T18309	111-115	VB	denotes	have
T18310	116-120	VB	denotes	been
T18311	121-130	VB	denotes	described
T18312	131-141	RB	denotes	previously
T18313	142-145	CC	denotes	and
T18314	146-150	VB	denotes	were
T18315	151-161	VB	denotes	maintained
T18316	162-164	IN	denotes	as
T18317	165-178	NN	denotes	heterozygotes
T18318	179-181	IN	denotes	on
T18319	182-183	DT	denotes	a
T18320	184-189	NN	denotes	Balbc
T18321	190-200	NN	denotes	background
T18322	201-202	-LRB-	denotes	[
T18323	202-204	CD	denotes	26
T18324	204-205	-RRB-	denotes	]
T18325	207-211	NN	denotes	LMP1
T18326	212-216	NN	denotes	mice
T18327	217-221	VB	denotes	were
T18328	222-231	VB	denotes	genotyped
T18329	232-234	IN	denotes	by
T18330	235-243	JJ	denotes	Southern
T18331	244-248	NN	denotes	blot
T18332	249-252	CC	denotes	and
T18333	253-256	NN	denotes	PCR
T18334	257-265	NN	denotes	analysis
T18335	266-268	IN	denotes	of
T18336	269-273	NN	denotes	tail
T18337	274-277	NN	denotes	DNA
T18338	278-280	IN	denotes	as
T18339	281-290	VB	denotes	described
T18340	291-301	RB	denotes	previously
T18341	302-303	-LRB-	denotes	[
T18342	303-305	CD	denotes	26
T18343	305-306	-RRB-	denotes	]
T18344	308-314	NN	denotes	Spleen
T18345	315-318	CC	denotes	and
T18346	319-324	NN	denotes	liver
T18347	325-333	NN	denotes	sections
T18348	334-338	VB	denotes	were
T18349	339-344	VB	denotes	fixed
T18350	345-347	IN	denotes	in
T18351	348-349	CD	denotes	4
T18352	349-350	NN	denotes	%
T18353	351-367	NN	denotes	paraformaldehyde
T18354	368-371	CC	denotes	and
T18355	372-380	VB	denotes	embedded
T18356	381-383	IN	denotes	in
T18357	384-392	NN	denotes	paraffin
T18358	392-393	-COMMA-	denotes	,
T18359	394-397	CC	denotes	and
T18360	398-402	JJ	denotes	5-μm
T18361	403-411	NN	denotes	sections
T18362	412-416	VB	denotes	were
T18363	417-424	VB	denotes	stained
T18364	425-429	IN	denotes	with
T18365	430-441	NN	denotes	hematoxylin
T18366	442-445	CC	denotes	and
T18367	446-451	NN	denotes	eosin
T18368	452-455	IN	denotes	for
T18369	456-473	JJ	denotes	histopathological
T18370	474-482	NN	denotes	analysis
T18371	484-493	NN	denotes	Lymphomas
T18372	494-498	VB	denotes	were
T18373	499-507	VB	denotes	passaged
T18374	508-510	IN	denotes	by
T18375	511-526	JJ	denotes	intraperitoneal
T18376	527-536	NN	denotes	injection
T18377	537-539	IN	denotes	of
T18378	540-541	CD	denotes	1
T18379	542-543	NN	denotes	×
T18380	544-547	CD	denotes	108
T18381	548-559	NN	denotes	splenocytes
T18382	560-564	IN	denotes	into
T18383	565-569	NN	denotes	SCID
T18384	570-574	NN	denotes	mice
T18385	575-578	CC	denotes	and
T18386	579-589	VB	denotes	sacrificed
T18387	590-594	IN	denotes	upon
T18388	595-606	NN	denotes	development
T18389	607-609	IN	denotes	of
T18390	610-612	DT	denotes	an
T18391	613-621	VB	denotes	extended
T18392	622-629	NN	denotes	abdomen
T18393	631-638	NN	denotes	Animals
T18394	639-643	VB	denotes	were
T18395	644-650	VB	denotes	housed
T18396	651-653	IN	denotes	in
T18397	654-657	DT	denotes	the
T18398	658-669	NNP	denotes	Association
T18399	670-673	IN	denotes	for
T18400	674-684	NNP	denotes	Assessment
T18401	685-688	CC	denotes	and
T18402	689-702	NNP	denotes	Accreditation
T18403	703-706	IN	denotes	for
T18404	707-713	NNP	denotes	Animal
T18405	714-727	NNP	denotes	Care–approved
T18406	728-734	NN	denotes	animal
T18407	735-743	NN	denotes	facility
T18408	744-746	IN	denotes	at
T18409	747-750	DT	denotes	the
T18410	751-761	NNP	denotes	University
T18411	762-764	IN	denotes	of
T18412	765-770	NNP	denotes	North
T18413	771-779	NNP	denotes	Carolina
T18414	780-782	IN	denotes	at
T18415	783-789	NNP	denotes	Chapel
T18416	790-795	NNP	denotes	Hill.
T18417	796-799	DT	denotes	All
T18418	800-809	NN	denotes	protocols
T18419	810-814	VB	denotes	were
T18420	815-823	VB	denotes	approved
T18421	824-826	IN	denotes	by
T18422	827-830	DT	denotes	the
T18423	831-844	NNP	denotes	Institutional
T18424	845-851	NNP	denotes	Animal
T18425	852-856	NNP	denotes	Care
T18426	857-860	CC	denotes	and
T18427	861-864	NNP	denotes	Use
T18428	865-874	NNP	denotes	Committee
T18617	877-886	NN	denotes	Isolation
T18618	887-890	CC	denotes	and
T18619	891-897	NN	denotes	growth
T18620	898-900	IN	denotes	of
T18621	901-902	NN	denotes	B
T18622	903-908	NN	denotes	cells
T18623	910-921	NN	denotes	Splenocytes
T18624	922-926	VB	denotes	were
T18625	927-935	VB	denotes	prepared
T18626	936-938	IN	denotes	by
T18627	939-951	VB	denotes	homogenizing
T18628	952-958	NN	denotes	spleen
T18629	959-965	NN	denotes	tissue
T18630	966-970	IN	denotes	with
T18631	971-974	CD	denotes	two
T18632	975-982	JJ	denotes	frosted
T18633	983-989	NN	denotes	slides
T18634	990-993	CC	denotes	and
T18635	994-1000	NN	denotes	debris
T18636	1001-1004	VB	denotes	was
T18637	1005-1013	VB	denotes	filtered
T18638	1014-1021	IN	denotes	through
T18639	1022-1023	DT	denotes	a
T18640	1024-1030	JJ	denotes	100-μm
T18641	1031-1035	NN	denotes	cell
T18642	1036-1044	NN	denotes	strainer
T18643	1046-1058	NN	denotes	Erythrocytes
T18644	1059-1063	VB	denotes	were
T18645	1064-1069	VB	denotes	lysed
T18646	1070-1075	VB	denotes	using
T18647	1076-1079	CD	denotes	0.8
T18648	1079-1080	NN	denotes	%
T18649	1081-1089	NN	denotes	ammonium
T18650	1090-1098	NN	denotes	chloride
T18651	1099-1107	NN	denotes	solution
T18652	1108-1109	-LRB-	denotes	(
T18653	1109-1117	NNP	denotes	StemCell
T18654	1118-1130	NNP	denotes	Technologies
T18655	1130-1131	-RRB-	denotes	)
T18656	1132-1135	IN	denotes	for
T18657	1136-1138	CD	denotes	10
T18658	1139-1142	NN	denotes	min
T18659	1143-1145	IN	denotes	on
T18660	1146-1149	NN	denotes	ice
T18661	1150-1153	CC	denotes	and
T18662	1154-1160	VB	denotes	washed
T18663	1161-1166	RB	denotes	twice
T18664	1167-1171	IN	denotes	with
T18665	1172-1175	NNP	denotes	PBS
T18666	1177-1178	NN	denotes	B
T18667	1179-1184	NN	denotes	cells
T18668	1185-1189	VB	denotes	were
T18669	1190-1198	VB	denotes	isolated
T18670	1199-1204	VB	denotes	using
T18671	1205-1214	JJ	denotes	CD19-MACS
T18672	1215-1220	NN	denotes	beads
T18673	1221-1230	VB	denotes	according
T18674	1231-1233	TO	denotes	to
T18675	1234-1237	DT	denotes	the
T18676	1238-1250	NN	denotes	manufacturer
T18677	1250-1252	POS	denotes	's
T18678	1253-1265	NN	denotes	instructions
T18679	1266-1267	-LRB-	denotes	(
T18680	1267-1275	NNP	denotes	Miltenyi
T18681	1276-1282	NNP	denotes	Biotec
T18682	1282-1283	-RRB-	denotes	)
T18683	1284-1287	CC	denotes	and
T18684	1288-1293	VB	denotes	grown
T18685	1294-1296	IN	denotes	in
T18686	1297-1303	NNP	denotes	Iscove
T18687	1303-1305	POS	denotes	's
T18688	1306-1312	NN	denotes	medium
T18689	1313-1325	VB	denotes	supplemented
T18690	1326-1330	IN	denotes	with
T18691	1331-1347	JJ	denotes	heat-inactivated
T18692	1348-1350	CD	denotes	10
T18693	1350-1351	NN	denotes	%
T18694	1352-1357	JJ	denotes	fetal
T18695	1358-1364	JJ	denotes	bovine
T18696	1365-1370	NN	denotes	serum
T18697	1371-1374	CC	denotes	and
T18698	1375-1397	JJ	denotes	antibiotic/antimycotic
T18699	1398-1399	-LRB-	denotes	(
T18700	1399-1404	NN	denotes	GIBCO
T18701	1404-1405	-RRB-	denotes	)
T18702	1407-1418	NN	denotes	Splenocytes
T18703	1419-1427	VB	denotes	isolated
T18704	1428-1432	IN	denotes	from
T18705	1433-1446	JJ	denotes	SCID-passaged
T18706	1447-1456	NN	denotes	lymphomas
T18707	1457-1466	VB	denotes	consisted
T18708	1467-1469	IN	denotes	of
T18709	1470-1472	CD	denotes	80
T18710	1472-1473	NN	denotes	%
T18711	1473-1476	CD	denotes	–90
T18712	1476-1477	NN	denotes	%
T18713	1478-1479	NN	denotes	B
T18714	1480-1485	NN	denotes	cells
T18715	1486-1488	IN	denotes	as
T18716	1489-1499	VB	denotes	determined
T18717	1500-1502	IN	denotes	by
T18718	1503-1507	NN	denotes	flow
T18719	1508-1517	NN	denotes	cytometry
T18720	1517-1518	-COMMA-	denotes	,
T18721	1519-1522	CC	denotes	and
T18722	1523-1527	VB	denotes	were
T18723	1528-1533	RB	denotes	hence
T18724	1534-1537	RB	denotes	not
T18725	1538-1545	RB	denotes	further
T18726	1546-1554	VB	denotes	purified
T18727	1555-1559	IN	denotes	with
T18728	1560-1569	JJ	denotes	CD19-MACS
T18729	1570-1575	NN	denotes	beads
T18730	1577-1588	NN	denotes	Splenocytes
T18731	1589-1593	VB	denotes	were
T18732	1594-1600	VB	denotes	seeded
T18733	1601-1603	IN	denotes	at
T18734	1604-1608	CD	denotes	1.25
T18735	1609-1610	CC	denotes	×
T18736	1611-1614	CD	denotes	106
T18737	1615-1623	NN	denotes	cells/ml
T18738	1624-1627	CC	denotes	and
T18739	1628-1633	WRB	denotes	where
T18740	1634-1644	JJ	denotes	applicable
T18741	1644-1645	-COMMA-	denotes	,
T18742	1646-1657	JJ	denotes	recombinant
T18743	1658-1663	NN	denotes	mouse
T18744	1664-1667	NN	denotes	IL4
T18745	1668-1671	VB	denotes	was
T18746	1672-1677	VB	denotes	added
T18747	1678-1680	IN	denotes	at
T18748	1681-1684	CD	denotes	100
T18749	1685-1690	NN	denotes	ng/ml
T18750	1690-1691	-COMMA-	denotes	,
T18751	1692-1703	JJ	denotes	recombinant
T18752	1704-1709	NN	denotes	mouse
T18753	1710-1714	NN	denotes	IL10
T18754	1715-1717	IN	denotes	at
T18755	1718-1720	CD	denotes	10
T18756	1721-1726	NN	denotes	ng/ml
T18757	1726-1727	-COMMA-	denotes	,
T18758	1728-1731	CC	denotes	and
T18759	1732-1735	NN	denotes	rat
T18760	1736-1740	NN	denotes	IgG1
T18761	1741-1751	JJ	denotes	anti-mouse
T18762	1752-1756	NN	denotes	IL10
T18763	1757-1760	CC	denotes	and
T18764	1761-1764	NN	denotes	rat
T18765	1765-1769	NN	denotes	IgG1
T18766	1770-1777	NN	denotes	isotype
T18767	1778-1785	NN	denotes	control
T18768	1786-1788	IN	denotes	at
T18769	1789-1791	CD	denotes	10
T18770	1792-1797	NN	denotes	μg/ml
T18771	1798-1799	-LRB-	denotes	(
T18772	1799-1800	NNP	denotes	R
T18773	1800-1801	CC	denotes	&
T18774	1801-1802	NNP	denotes	D
T18775	1803-1810	NNP	denotes	Systems
T18776	1810-1811	-RRB-	denotes	)
T18777	1813-1816	IN	denotes	For
T18778	1817-1821	NN	denotes	BrdU
T18779	1822-1835	NN	denotes	incorporation
T18780	1836-1842	NN	denotes	assays
T18781	1842-1843	-COMMA-	denotes	,
T18782	1844-1855	NN	denotes	splenocytes
T18783	1856-1860	VB	denotes	were
T18784	1861-1867	VB	denotes	pulsed
T18785	1868-1871	IN	denotes	for
T18786	1872-1874	CD	denotes	24
T18787	1875-1876	NN	denotes	h
T18788	1877-1881	IN	denotes	with
T18789	1882-1884	CD	denotes	10
T18790	1885-1887	NNP	denotes	μM
T18791	1888-1892	NNP	denotes	BrdU
T18792	1893-1894	CD	denotes	1
T18793	1895-1896	NN	denotes	d
T18794	1897-1909	JJ	denotes	post-harvest
T19051	1912-1915	NN	denotes	MTS
T19052	1916-1921	NN	denotes	assay
T19053	1923-1926	DT	denotes	The
T19054	1927-1929	CD	denotes	3-
T19055	1929-1930	-LRB-	denotes	(
T19056	1930-1931	CD	denotes	4
T19057	1931-1932	-COMMA-	denotes	,
T19058	1933-1955	NN	denotes	5-dimethylthiazol-2-yl
T19059	1955-1956	-RRB-	denotes	)
T19060	1956-1959	CD	denotes	-5-
T19061	1959-1960	-LRB-	denotes	(
T19062	1960-1982	NN	denotes	3-carboxymethoxyphenyl
T19063	1982-1983	-RRB-	denotes	)
T19064	1983-1986	CD	denotes	-2-
T19065	1986-1987	-LRB-	denotes	(
T19066	1987-2000	NN	denotes	4-sulfophenyl
T19067	2000-2001	-RRB-	denotes	)
T19068	2001-2016	NN	denotes	-2H-tetrazolium
T19069	2017-2022	JJ	denotes	inner
T19070	2023-2027	NN	denotes	salt
T19071	2028-2029	-LRB-	denotes	(
T19072	2029-2032	NN	denotes	MTS
T19073	2032-2033	-RRB-	denotes	)
T19074	2034-2038	NN	denotes	cell
T19075	2039-2065	NN	denotes	cytotoxicity/proliferation
T19076	2066-2072	NN	denotes	assays
T19077	2073-2077	VB	denotes	were
T19078	2078-2087	VB	denotes	performed
T19079	2088-2093	VB	denotes	using
T19080	2094-2097	DT	denotes	the
T19081	2098-2107	NNP	denotes	CellTiter
T19082	2108-2110	CD	denotes	96
T19083	2111-2118	JJ	denotes	aqueous
T19084	2119-2131	NN	denotes	one-solution
T19085	2132-2136	NN	denotes	cell
T19086	2137-2150	NN	denotes	proliferation
T19087	2151-2156	NN	denotes	assay
T19088	2157-2158	-LRB-	denotes	(
T19089	2158-2165	NN	denotes	Promega
T19090	2165-2166	-RRB-	denotes	)
T19091	2166-2167	-COMMA-	denotes	,
T19092	2168-2177	VB	denotes	according
T19093	2178-2180	TO	denotes	to
T19094	2181-2193	NN	denotes	manufacturer
T19095	2193-2195	POS	denotes	's
T19096	2196-2208	NN	denotes	instructions
T19097	2210-2213	IN	denotes	For
T19098	2214-2217	NN	denotes	IL4
T19099	2218-2225	NN	denotes	studies
T19100	2225-2226	-COMMA-	denotes	,
T19101	2227-2238	NN	denotes	splenocytes
T19102	2239-2243	VB	denotes	were
T19103	2244-2252	VB	denotes	cultured
T19104	2253-2256	IN	denotes	for
T19105	2257-2258	CD	denotes	3
T19106	2259-2260	NN	denotes	d
T19107	2261-2263	IN	denotes	in
T19108	2264-2267	DT	denotes	the
T19109	2268-2276	NN	denotes	presence
T19110	2277-2279	CC	denotes	or
T19111	2280-2287	NN	denotes	absence
T19112	2288-2290	IN	denotes	of
T19113	2291-2294	CD	denotes	100
T19114	2295-2300	NN	denotes	ng/ml
T19115	2301-2304	NN	denotes	IL4
T19116	2306-2311	NN	denotes	Cells
T19117	2312-2316	VB	denotes	were
T19118	2317-2323	VB	denotes	seeded
T19119	2324-2326	IN	denotes	on
T19120	2327-2330	NN	denotes	day
T19121	2331-2332	CD	denotes	3
T19122	2333-2335	IN	denotes	in
T19123	2336-2346	NN	denotes	triplicate
T19124	2347-2349	IN	denotes	in
T19125	2350-2351	DT	denotes	a
T19126	2352-2359	JJ	denotes	96-well
T19127	2360-2365	NN	denotes	plate
T19128	2366-2368	IN	denotes	at
T19129	2369-2372	CD	denotes	2.5
T19130	2373-2374	CC	denotes	×
T19131	2375-2378	CD	denotes	106
T19132	2379-2387	NN	denotes	cells/ml
T19133	2388-2390	IN	denotes	at
T19134	2391-2394	CD	denotes	100
T19135	2395-2397	NN	denotes	μl
T19136	2398-2401	IN	denotes	per
T19137	2402-2406	RB	denotes	well
T19138	2408-2411	NN	denotes	MTS
T19139	2412-2419	NN	denotes	reagent
T19140	2420-2423	VB	denotes	was
T19141	2424-2429	VB	denotes	added
T19142	2430-2433	IN	denotes	for
T19143	2434-2435	CD	denotes	4
T19144	2436-2437	NN	denotes	h
T19145	2438-2441	CC	denotes	and
T19146	2442-2452	NN	denotes	absorbance
T19147	2453-2456	VB	denotes	was
T19148	2457-2461	VB	denotes	read
T19149	2462-2464	IN	denotes	at
T19150	2465-2468	CD	denotes	540
T19151	2469-2471	NN	denotes	nm
T19152	2471-2472	-COLON-	denotes	;
T19153	2473-2479	NN	denotes	values
T19154	2480-2487	VB	denotes	plotted
T19155	2488-2492	VB	denotes	were
T19156	2493-2503	VB	denotes	subtracted
T19157	2504-2508	IN	denotes	from
T19158	2509-2515	NN	denotes	blanks
T19159	2517-2520	IN	denotes	For
T19160	2521-2535	NN	denotes	neutralization
T19161	2536-2542	NN	denotes	assays
T19162	2542-2543	-COMMA-	denotes	,
T19163	2544-2555	NN	denotes	splenocytes
T19164	2556-2560	VB	denotes	were
T19165	2561-2567	VB	denotes	seeded
T19166	2568-2570	IN	denotes	at
T19167	2571-2572	CD	denotes	5
T19168	2573-2574	-COLON-	denotes	×
T19169	2575-2578	CD	denotes	106
T19170	2579-2587	NN	denotes	cells/ml
T19171	2588-2590	IN	denotes	at
T19172	2591-2594	CD	denotes	100
T19173	2595-2597	NN	denotes	μl
T19174	2598-2601	IN	denotes	per
T19175	2602-2606	RB	denotes	well
T19176	2607-2609	IN	denotes	on
T19177	2610-2613	NN	denotes	day
T19178	2614-2616	IN	denotes	of
T19179	2617-2624	NN	denotes	harvest
T19180	2625-2628	CC	denotes	and
T19181	2629-2632	NN	denotes	IL4
T19182	2632-2633	-COMMA-	denotes	,
T19183	2634-2637	NN	denotes	rat
T19184	2638-2642	NN	denotes	IgG1
T19185	2643-2653	JJ	denotes	anti-mouse
T19186	2654-2657	NN	denotes	IL4
T19187	2657-2658	-COMMA-	denotes	,
T19188	2659-2661	CC	denotes	or
T19189	2662-2665	NN	denotes	rat
T19190	2666-2670	NN	denotes	IgG1
T19191	2671-2678	NN	denotes	isotype
T19192	2679-2686	NN	denotes	control
T19193	2687-2688	-LRB-	denotes	(
T19194	2688-2689	NNP	denotes	R
T19195	2689-2690	CC	denotes	&
T19196	2690-2691	NNP	denotes	D
T19197	2692-2699	NNP	denotes	Systems
T19198	2699-2700	-RRB-	denotes	)
T19199	2701-2705	VB	denotes	were
T19200	2706-2711	VB	denotes	added
T19201	2712-2714	IN	denotes	at
T19202	2715-2718	DT	denotes	the
T19203	2719-2733	NN	denotes	concentrations
T19204	2734-2743	VB	denotes	indicated
T19205	2744-2746	IN	denotes	in
T19206	2747-2750	DT	denotes	the
T19207	2751-2758	NN	denotes	figures
T19208	2760-2768	NN	denotes	Cultures
T19209	2769-2773	VB	denotes	were
T19210	2774-2780	VB	denotes	pulsed
T19211	2781-2784	IN	denotes	for
T19212	2785-2786	CD	denotes	4
T19213	2787-2788	NN	denotes	h
T19214	2789-2793	IN	denotes	with
T19215	2794-2797	NN	denotes	MTS
T19216	2798-2805	NN	denotes	reagent
T19217	2806-2807	CD	denotes	1
T19218	2808-2809	NN	denotes	d
T19219	2810-2822	JJ	denotes	post-seeding
T19220	2824-2827	IN	denotes	For
T19221	2828-2837	NN	denotes	inhibitor
T19222	2838-2845	NN	denotes	studies
T19223	2845-2846	-COMMA-	denotes	,
T19224	2847-2858	NN	denotes	splenocytes
T19225	2859-2863	VB	denotes	were
T19226	2864-2870	VB	denotes	seeded
T19227	2871-2873	IN	denotes	at
T19228	2874-2875	CD	denotes	1
T19229	2876-2877	NN	denotes	×
T19230	2878-2881	CD	denotes	107
T19231	2882-2890	NN	denotes	cells/ml
T19232	2891-2893	IN	denotes	at
T19233	2894-2897	CD	denotes	100
T19234	2898-2900	NN	denotes	μl
T19235	2901-2904	IN	denotes	per
T19236	2905-2909	RB	denotes	well
T19237	2910-2912	IN	denotes	on
T19238	2913-2916	NN	denotes	day
T19239	2917-2919	IN	denotes	of
T19240	2920-2927	NN	denotes	harvest
T19241	2927-2928	-COMMA-	denotes	,
T19242	2929-2932	CC	denotes	and
T19243	2933-2943	NN	denotes	inhibitors
T19244	2944-2948	VB	denotes	were
T19245	2949-2954	VB	denotes	added
T19246	2955-2957	IN	denotes	at
T19247	2958-2961	DT	denotes	the
T19248	2962-2976	NN	denotes	concentrations
T19249	2977-2986	VB	denotes	indicated
T19250	2987-2989	IN	denotes	in
T19251	2990-2993	DT	denotes	the
T19252	2994-3001	NN	denotes	figures
T19253	3003-3006	DT	denotes	The
T19254	3007-3017	NN	denotes	inhibitors
T19255	3018-3028	NN	denotes	BAY11–7085
T19256	3028-3029	-COMMA-	denotes	,
T19257	3030-3039	NN	denotes	rapamycin
T19258	3039-3040	-COMMA-	denotes	,
T19259	3041-3052	NN	denotes	triciribine
T19260	3052-3053	-COMMA-	denotes	,
T19261	3054-3059	NN	denotes	U0126
T19262	3059-3060	-COMMA-	denotes	,
T19263	3061-3069	NN	denotes	SB202190
T19264	3069-3070	-COMMA-	denotes	,
T19265	3071-3074	CC	denotes	and
T19266	3075-3087	NN	denotes	cucurbitacin
T19267	3088-3089	PRP	denotes	I
T19268	3090-3094	VB	denotes	were
T19269	3095-3104	VB	denotes	purchased
T19270	3105-3109	IN	denotes	from
T19271	3110-3113	NNP	denotes	EMD
T19272	3114-3126	NNP	denotes	Biosciences.
T19273	3127-3130	IN	denotes	For
T19274	3131-3144	JJ	denotes	non-malignant
T19275	3145-3155	NN	denotes	splenocyte
T19276	3156-3164	NN	denotes	cultures
T19277	3164-3165	-COMMA-	denotes	,
T19278	3166-3167	NN	denotes	B
T19279	3168-3172	NN	denotes	cell
T19280	3173-3183	NN	denotes	activation
T19281	3184-3187	VB	denotes	was
T19282	3188-3195	VB	denotes	induced
T19283	3196-3200	IN	denotes	with
T19284	3201-3203	CD	denotes	10
T19285	3204-3209	NN	denotes	μg/ml
T19286	3210-3212	IN	denotes	of
T19287	3213-3217	NN	denotes	goat
T19288	3218-3219	NN	denotes	F
T19289	3219-3220	-LRB-	denotes	(
T19290	3220-3222	NN	denotes	ab
T19291	3222-3223	-DQE-	denotes	'
T19292	3223-3224	-RRB-	denotes	)
T19293	3225-3235	JJ	denotes	anti-mouse
T19294	3236-3239	NN	denotes	IgM
T19295	3240-3241	-LRB-	denotes	(
T19296	3241-3248	NNP	denotes	Jackson
T19297	3249-3263	NNP	denotes	ImmunoResearch
T19298	3263-3264	-RRB-	denotes	)
T19299	3266-3274	NN	denotes	Cultures
T19300	3275-3279	VB	denotes	were
T19301	3280-3286	VB	denotes	pulsed
T19302	3287-3290	IN	denotes	for
T19303	3291-3292	CD	denotes	4
T19304	3293-3294	NN	denotes	h
T19305	3295-3299	IN	denotes	with
T19306	3300-3303	NN	denotes	MTS
T19307	3304-3311	NN	denotes	reagent
T19308	3312-3313	CD	denotes	1
T19309	3314-3315	NN	denotes	d
T19310	3316-3328	JJ	denotes	post-seeding
T19650	3331-3351	NN	denotes	Immunohistochemistry
T19651	3353-3370	JJ	denotes	Paraffin-embedded
T19652	3371-3377	NN	denotes	spleen
T19653	3378-3386	NN	denotes	sections
T19654	3387-3391	VB	denotes	were
T19655	3392-3406	VB	denotes	deparaffinized
T19656	3407-3409	IN	denotes	in
T19657	3410-3420	JJ	denotes	Histoclear
T19658	3421-3422	-LRB-	denotes	(
T19659	3422-3430	NNP	denotes	National
T19660	3431-3442	NNP	denotes	Diagnostics
T19661	3442-3443	-RRB-	denotes	)
T19662	3444-3447	CC	denotes	and
T19663	3448-3458	VB	denotes	rehydrated
T19664	3459-3461	IN	denotes	in
T19665	3462-3468	VB	denotes	graded
T19666	3469-3476	NN	denotes	ethanol
T19667	3478-3486	NN	denotes	Sections
T19668	3487-3491	VB	denotes	were
T19669	3492-3499	NN	denotes	antigen
T19670	3500-3509	VB	denotes	retrieved
T19671	3510-3512	IN	denotes	by
T19672	3513-3524	VB	denotes	microwaving
T19673	3525-3527	IN	denotes	in
T19674	3528-3535	NN	denotes	citrate
T19675	3536-3542	NN	denotes	buffer
T19676	3543-3544	-LRB-	denotes	(
T19677	3544-3546	NN	denotes	pH
T19678	3547-3550	CD	denotes	6.0
T19679	3550-3551	-RRB-	denotes	)
T19680	3552-3555	IN	denotes	for
T19681	3556-3558	CD	denotes	15
T19682	3559-3562	NN	denotes	min
T19683	3563-3564	-LRB-	denotes	(
T19684	3564-3568	NN	denotes	LMP1
T19685	3569-3577	NN	denotes	staining
T19686	3577-3578	-RRB-	denotes	)
T19687	3579-3582	CC	denotes	and
T19688	3583-3585	CD	denotes	10
T19689	3586-3589	NN	denotes	min
T19690	3590-3591	-LRB-	denotes	(
T19691	3591-3597	NN	denotes	pStat3
T19692	3598-3606	NN	denotes	staining
T19693	3606-3607	-RRB-	denotes	)
T19694	3609-3612	IN	denotes	For
T19695	3613-3617	NN	denotes	LMP1
T19696	3618-3626	NN	denotes	staining
T19697	3626-3627	-COMMA-	denotes	,
T19698	3628-3636	NN	denotes	sections
T19699	3637-3641	VB	denotes	were
T19700	3642-3649	VB	denotes	blocked
T19701	3650-3654	IN	denotes	with
T19702	3655-3656	CD	denotes	1
T19703	3656-3657	NN	denotes	%
T19704	3658-3661	NN	denotes	BSA
T19705	3662-3665	CC	denotes	and
T19706	3666-3669	CD	denotes	0.1
T19707	3669-3670	NN	denotes	%
T19708	3671-3675	JJ	denotes	cold
T19709	3676-3680	NN	denotes	fish
T19710	3681-3685	NN	denotes	skin
T19711	3686-3693	NN	denotes	gelatin
T19712	3694-3702	VB	denotes	followed
T19713	3703-3707	IN	denotes	with
T19714	3708-3727	NN	denotes	streptavidin/biotin
T19715	3728-3733	NN	denotes	block
T19716	3734-3735	-LRB-	denotes	(
T19717	3735-3741	NNP	denotes	Vector
T19718	3742-3746	NNP	denotes	Labs
T19719	3746-3747	-RRB-	denotes	)
T19720	3749-3752	NN	denotes	Rat
T19721	3753-3756	NN	denotes	IgG
T19722	3757-3766	NN	denotes	anti-LMP1
T19723	3767-3768	-LRB-	denotes	(
T19724	3768-3774	NN	denotes	clones
T19725	3775-3778	NN	denotes	8G3
T19726	3779-3782	CC	denotes	and
T19727	3783-3786	NN	denotes	1G6
T19728	3786-3787	-COMMA-	denotes	,
T19729	3788-3796	NN	denotes	Ascenion
T19730	3796-3797	-RRB-	denotes	)
T19731	3798-3802	VB	denotes	were
T19732	3803-3807	VB	denotes	used
T19733	3808-3810	IN	denotes	at
T19734	3811-3812	CD	denotes	1
T19735	3812-3813	SYM	denotes	:
T19736	3813-3815	CD	denotes	10
T19737	3816-3824	NN	denotes	dilution
T19738	3825-3829	IN	denotes	from
T19739	3830-3836	NN	denotes	tissue
T19740	3837-3844	NN	denotes	culture
T19741	3845-3857	NN	denotes	supernatants
T19742	3857-3858	-COMMA-	denotes	,
T19743	3859-3867	VB	denotes	followed
T19744	3868-3872	IN	denotes	with
T19745	3873-3874	CD	denotes	8
T19746	3875-3880	NN	denotes	μg/ml
T19747	3881-3893	VB	denotes	biotinylated
T19748	3894-3899	NN	denotes	mouse
T19749	3900-3901	NN	denotes	F
T19750	3901-3902	-LRB-	denotes	(
T19751	3902-3904	NN	denotes	ab
T19752	3904-3905	-DQE-	denotes	'
T19753	3905-3906	-RRB-	denotes	)
T19754	3907-3915	JJ	denotes	anti-rat
T19755	3916-3919	NN	denotes	IgG
T19756	3920-3921	-LRB-	denotes	(
T19757	3921-3924	NN	denotes	H+L
T19758	3924-3925	-RRB-	denotes	)
T19759	3926-3938	JJ	denotes	pre-adsorbed
T19760	3939-3941	TO	denotes	to
T19761	3942-3947	NN	denotes	mouse
T19762	3948-3953	NN	denotes	serum
T19763	3954-3955	-LRB-	denotes	(
T19764	3955-3962	NNP	denotes	Jackson
T19765	3963-3977	NNP	denotes	ImmunoResearch
T19766	3977-3978	-RRB-	denotes	)
T19767	3979-3982	CC	denotes	and
T19768	3983-3984	CD	denotes	2
T19769	3985-3990	NN	denotes	μg/ml
T19770	3991-4012	JJ	denotes	streptavidin-alkaline
T19771	4013-4024	NN	denotes	phosphatase
T19772	4025-4034	NN	denotes	conjugate
T19773	4035-4036	-LRB-	denotes	(
T19774	4036-4043	NNP	denotes	Jackson
T19775	4044-4058	NNP	denotes	ImmunoResearch
T19776	4058-4059	-RRB-	denotes	)
T19777	4061-4067	NN	denotes	Stains
T19778	4068-4072	VB	denotes	were
T19779	4073-4082	VB	denotes	developed
T19780	4083-4087	IN	denotes	with
T19781	4088-4096	NN	denotes	BCIP/NBT
T19782	4097-4100	CC	denotes	and
T19783	4101-4115	VB	denotes	counterstained
T19784	4116-4118	IN	denotes	in
T19785	4119-4126	NNP	denotes	Nuclear
T19786	4127-4131	NNP	denotes	Fast
T19787	4132-4135	NNP	denotes	Red
T19788	4136-4137	-LRB-	denotes	(
T19789	4137-4141	NNP	denotes	Dako
T19790	4141-4142	-RRB-	denotes	)
T19791	4144-4157	NN	denotes	Phospho-Stat3
T19792	4158-4161	VB	denotes	was
T19793	4162-4170	VB	denotes	detected
T19794	4171-4175	IN	denotes	with
T19795	4176-4177	CD	denotes	4
T19796	4178-4183	NN	denotes	μg/ml
T19797	4184-4186	IN	denotes	of
T19798	4187-4193	NN	denotes	pStat3
T19799	4194-4202	NN	denotes	antibody
T19800	4203-4204	-LRB-	denotes	(
T19801	4204-4210	NN	denotes	Tyr705
T19802	4210-4211	-COMMA-	denotes	,
T19803	4212-4216	NNP	denotes	Cell
T19804	4217-4226	NNP	denotes	Signaling
T19805	4226-4227	-RRB-	denotes	)
T19806	4228-4231	CC	denotes	and
T19807	4232-4240	VB	denotes	detected
T19808	4241-4245	IN	denotes	with
T19809	4246-4257	JJ	denotes	anti-rabbit
T19810	4258-4266	NN	denotes	Poly-HRP
T19811	4267-4270	NN	denotes	IHC
T19812	4271-4280	NN	denotes	detection
T19813	4281-4284	NN	denotes	kit
T19814	4285-4286	-LRB-	denotes	(
T19815	4286-4294	NNP	denotes	Chemicon
T19816	4294-4295	-RRB-	denotes	)
T20045	4298-4302	NN	denotes	Flow
T20046	4303-4312	NN	denotes	cytometry
T20047	4314-4317	CD	denotes	One
T20048	4318-4325	CD	denotes	million
T20049	4326-4337	NN	denotes	splenocytes
T20050	4338-4342	VB	denotes	were
T20051	4343-4350	VB	denotes	stained
T20052	4351-4355	IN	denotes	with
T20053	4356-4359	DT	denotes	the
T20054	4360-4371	JJ	denotes	appropriate
T20055	4372-4379	JJ	denotes	primary
T20056	4380-4388	NN	denotes	antibody
T20057	4389-4401	JJ	denotes	unconjugated
T20058	4402-4404	CC	denotes	or
T20059	4405-4415	VB	denotes	conjugated
T20060	4416-4418	TO	denotes	to
T20061	4419-4423	NN	denotes	FITC
T20062	4423-4424	-COMMA-	denotes	,
T20063	4425-4427	NN	denotes	PE
T20064	4427-4428	-COMMA-	denotes	,
T20065	4429-4431	CC	denotes	or
T20066	4432-4435	NN	denotes	APC
T20067	4436-4443	VB	denotes	diluted
T20068	4444-4446	IN	denotes	in
T20069	4447-4452	NN	denotes	stain
T20070	4453-4459	NN	denotes	buffer
T20071	4460-4461	-LRB-	denotes	(
T20072	4461-4464	NN	denotes	PBS
T20073	4465-4469	IN	denotes	with
T20074	4470-4471	CD	denotes	3
T20075	4471-4472	NN	denotes	%
T20076	4473-4476	NN	denotes	FBS
T20077	4476-4477	-RRB-	denotes	)
T20078	4479-4482	IN	denotes	For
T20079	4483-4487	NN	denotes	BrdU
T20080	4488-4497	NN	denotes	detection
T20081	4497-4498	-COMMA-	denotes	,
T20082	4499-4502	DT	denotes	the
T20083	4503-4512	NN	denotes	FITC-BrdU
T20084	4513-4517	NN	denotes	flow
T20085	4518-4521	NN	denotes	kit
T20086	4522-4525	VB	denotes	was
T20087	4526-4530	VB	denotes	used
T20088	4531-4533	IN	denotes	as
T20089	4534-4544	VB	denotes	instructed
T20090	4545-4547	IN	denotes	by
T20091	4548-4551	DT	denotes	the
T20092	4552-4564	NN	denotes	manufacturer
T20093	4565-4566	-LRB-	denotes	(
T20094	4566-4568	NN	denotes	BD
T20095	4569-4579	NN	denotes	Bioscience
T20096	4579-4580	-RRB-	denotes	)
T20097	4582-4589	RB	denotes	Briefly
T20098	4589-4590	-COMMA-	denotes	,
T20099	4591-4596	NN	denotes	cells
T20100	4597-4601	VB	denotes	were
T20101	4602-4609	VB	denotes	exposed
T20102	4610-4612	TO	denotes	to
T20103	4613-4616	NN	denotes	EMA
T20104	4617-4618	-LRB-	denotes	(
T20105	4618-4627	NNP	denotes	Molecular
T20106	4628-4634	NNP	denotes	Probes
T20107	4634-4635	-RRB-	denotes	)
T20108	4636-4639	IN	denotes	for
T20109	4640-4649	NN	denotes	exclusion
T20110	4650-4652	IN	denotes	of
T20111	4653-4657	JJ	denotes	dead
T20112	4658-4663	NN	denotes	cells
T20113	4663-4664	-COMMA-	denotes	,
T20114	4665-4672	VB	denotes	stained
T20115	4673-4676	IN	denotes	for
T20116	4677-4684	NN	denotes	surface
T20117	4685-4693	NN	denotes	antigens
T20118	4693-4694	-COMMA-	denotes	,
T20119	4695-4700	VB	denotes	fixed
T20120	4701-4703	IN	denotes	in
T20121	4704-4720	NN	denotes	paraformaldehyde
T20122	4720-4721	-COMMA-	denotes	,
T20123	4722-4725	CC	denotes	and
T20124	4726-4739	VB	denotes	permeabilized
T20125	4740-4744	IN	denotes	with
T20126	4745-4752	NN	denotes	saponin
T20127	4754-4756	TO	denotes	To
T20128	4757-4763	VB	denotes	expose
T20129	4764-4768	NNP	denotes	BrdU
T20130	4769-4777	NN	denotes	epitopes
T20131	4777-4778	-COMMA-	denotes	,
T20132	4779-4784	NN	denotes	cells
T20133	4785-4789	VB	denotes	were
T20134	4790-4797	VB	denotes	treated
T20135	4798-4802	IN	denotes	with
T20136	4803-4808	NN	denotes	Dnase
T20137	4809-4812	CC	denotes	and
T20138	4813-4820	VB	denotes	stained
T20139	4821-4825	IN	denotes	with
T20140	4826-4841	JJ	denotes	FITC-conjugated
T20141	4842-4851	JJ	denotes	anti-BrdU
T20142	4852-4860	NN	denotes	antibody
T20143	4862-4866	NN	denotes	Flow
T20144	4867-4876	NN	denotes	cytometry
T20145	4877-4880	VB	denotes	was
T20146	4881-4890	VB	denotes	performed
T20147	4891-4893	IN	denotes	on
T20148	4894-4905	NNP	denotes	FACScalibur
T20149	4906-4911	VB	denotes	using
T20150	4912-4915	DT	denotes	the
T20151	4916-4925	NN	denotes	CellQuest
T20152	4926-4933	NN	denotes	program
T20153	4934-4935	-LRB-	denotes	(
T20154	4935-4941	NNP	denotes	Becton
T20155	4942-4951	NNP	denotes	Dickinson
T20156	4951-4952	-RRB-	denotes	)
T20157	4954-4961	JJ	denotes	Further
T20158	4962-4970	NN	denotes	analysis
T20159	4971-4974	VB	denotes	was
T20160	4975-4984	VB	denotes	conducted
T20161	4985-4987	IN	denotes	on
T20162	4988-4991	DT	denotes	the
T20163	4992-4998	NNP	denotes	Summit
T20164	4999-5003	NN	denotes	v4.2
T20165	5004-5011	NN	denotes	program
T20166	5012-5013	-LRB-	denotes	(
T20167	5013-5017	NN	denotes	Dako
T20168	5017-5018	-RRB-	denotes	)
T20344	5021-5026	NN	denotes	Rnase
T20345	5027-5037	NN	denotes	protection
T20346	5038-5043	NN	denotes	assay
T20347	5045-5050	JJ	denotes	Total
T20348	5051-5054	NN	denotes	RNA
T20349	5055-5058	VB	denotes	was
T20350	5059-5067	VB	denotes	isolated
T20351	5068-5072	IN	denotes	from
T20352	5073-5078	JJ	denotes	CD19+
T20353	5079-5092	JJ	denotes	MACS-purified
T20354	5093-5094	NN	denotes	B
T20355	5095-5100	NN	denotes	cells
T20356	5101-5106	VB	denotes	using
T20357	5107-5110	DT	denotes	the
T20358	5111-5117	NNP	denotes	Rneasy
T20359	5118-5122	NN	denotes	midi
T20360	5123-5135	NN	denotes	purification
T20361	5136-5139	NN	denotes	kit
T20362	5140-5144	IN	denotes	with
T20363	5145-5150	NN	denotes	Dnase
T20364	5151-5160	NN	denotes	treatment
T20365	5160-5161	-COMMA-	denotes	,
T20366	5162-5171	VB	denotes	according
T20367	5172-5174	TO	denotes	to
T20368	5175-5178	DT	denotes	the
T20369	5179-5191	NN	denotes	manufacturer
T20370	5191-5193	POS	denotes	's
T20371	5194-5206	NN	denotes	instructions
T20372	5207-5208	-LRB-	denotes	(
T20373	5208-5214	NN	denotes	Qiagen
T20374	5214-5215	-RRB-	denotes	)
T20375	5217-5220	DT	denotes	The
T20376	5221-5226	NN	denotes	mCK-1
T20377	5227-5232	NN	denotes	probe
T20378	5233-5241	NN	denotes	template
T20379	5242-5245	NN	denotes	set
T20380	5246-5247	-LRB-	denotes	(
T20381	5247-5249	NN	denotes	BD
T20382	5250-5261	NNP	denotes	Biosciences
T20383	5261-5262	-RRB-	denotes	)
T20384	5263-5266	VB	denotes	was
T20385	5267-5274	VB	denotes	labeled
T20386	5275-5280	VB	denotes	using
T20387	5281-5284	DT	denotes	the
T20388	5285-5287	FW	denotes	In
T20389	5288-5293	FW	denotes	Vitro
T20390	5294-5307	NN	denotes	Transcription
T20391	5308-5311	NN	denotes	Kit
T20392	5312-5321	VB	denotes	according
T20393	5322-5324	TO	denotes	to
T20394	5325-5328	DT	denotes	the
T20395	5329-5341	NN	denotes	manufacturer
T20396	5341-5343	POS	denotes	's
T20397	5344-5356	NN	denotes	instructions
T20398	5357-5358	-LRB-	denotes	(
T20399	5358-5360	NN	denotes	BD
T20400	5361-5372	NNP	denotes	Biosciences
T20401	5372-5373	-RRB-	denotes	)
T20402	5375-5382	RB	denotes	Briefly
T20403	5382-5383	-COMMA-	denotes	,
T20404	5384-5386	CD	denotes	50
T20405	5387-5389	NN	denotes	ng
T20406	5390-5392	IN	denotes	of
T20407	5393-5398	NN	denotes	mCK-1
T20408	5399-5404	NN	denotes	probe
T20409	5405-5408	NN	denotes	set
T20410	5409-5412	VB	denotes	was
T20411	5413-5420	VB	denotes	labeled
T20412	5421-5425	IN	denotes	with
T20413	5426-5427	-LRB-	denotes	[
T20414	5427-5432	NN	denotes	α-32P
T20415	5432-5433	-RRB-	denotes	]
T20416	5433-5436	NN	denotes	UTP
T20417	5437-5442	VB	denotes	using
T20418	5443-5445	NN	denotes	T7
T20419	5446-5449	NN	denotes	RNA
T20420	5450-5460	NN	denotes	polymerase
T20421	5461-5464	CC	denotes	and
T20422	5465-5473	VB	denotes	purified
T20423	5474-5479	VB	denotes	using
T20424	5480-5488	NN	denotes	Sephadex
T20425	5489-5493	NN	denotes	G-50
T20426	5494-5501	NN	denotes	columns
T20427	5502-5503	-LRB-	denotes	(
T20428	5503-5510	NNP	denotes	NucAway
T20429	5511-5515	NNP	denotes	Spin
T20430	5516-5522	NN	denotes	column
T20431	5522-5523	-COMMA-	denotes	,
T20432	5524-5530	NNP	denotes	Ambion
T20433	5530-5531	-RRB-	denotes	)
T20434	5533-5536	DT	denotes	The
T20435	5537-5544	VB	denotes	labeled
T20436	5545-5550	NN	denotes	probe
T20437	5551-5554	VB	denotes	was
T20438	5555-5566	VB	denotes	quantitated
T20439	5567-5572	VB	denotes	using
T20440	5573-5574	DT	denotes	a
T20441	5575-5588	NN	denotes	scintillation
T20442	5589-5596	NN	denotes	counter
T20443	5596-5597	-COMMA-	denotes	,
T20444	5598-5601	CC	denotes	and
T20445	5602-5603	CD	denotes	6
T20446	5604-5605	SYM	denotes	×
T20447	5606-5609	CD	denotes	105
T20448	5610-5619	NN	denotes	cherenkov
T20449	5620-5623	NN	denotes	cpm
T20450	5624-5627	VB	denotes	was
T20451	5628-5632	VB	denotes	used
T20452	5633-5635	TO	denotes	to
T20453	5636-5645	VB	denotes	hybridize
T20454	5646-5648	TO	denotes	to
T20455	5649-5650	CD	denotes	4
T20456	5651-5653	NN	denotes	μg
T20457	5654-5656	IN	denotes	of
T20458	5657-5662	JJ	denotes	total
T20459	5663-5666	NN	denotes	RNA
T20460	5667-5672	VB	denotes	using
T20461	5673-5676	DT	denotes	the
T20462	5677-5680	NN	denotes	RPA
T20463	5681-5684	NN	denotes	kit
T20464	5685-5686	-LRB-	denotes	(
T20465	5686-5688	NN	denotes	BD
T20466	5689-5700	NNP	denotes	Biosciences
T20467	5700-5701	-RRB-	denotes	)
T20468	5703-5710	NN	denotes	Samples
T20469	5711-5715	VB	denotes	were
T20470	5716-5725	VB	denotes	denatured
T20471	5726-5728	IN	denotes	at
T20472	5729-5731	CD	denotes	90
T20473	5732-5734	NN	denotes	°C
T20474	5735-5738	CC	denotes	and
T20475	5739-5749	VB	denotes	hybridized
T20476	5750-5752	IN	denotes	at
T20477	5753-5755	CD	denotes	56
T20478	5756-5758	NN	denotes	°C
T20479	5759-5768	RB	denotes	overnight
T20480	5770-5785	JJ	denotes	Single-stranded
T20481	5786-5789	NN	denotes	RNA
T20482	5790-5793	VB	denotes	was
T20483	5794-5802	VB	denotes	digested
T20484	5803-5807	IN	denotes	with
T20485	5808-5809	DT	denotes	a
T20486	5810-5817	NN	denotes	mixture
T20487	5818-5820	IN	denotes	of
T20488	5821-5826	NN	denotes	Rnase
T20489	5827-5828	NN	denotes	A
T20490	5829-5832	CC	denotes	and
T20491	5833-5835	NN	denotes	T1
T20492	5835-5836	-COMMA-	denotes	,
T20493	5837-5840	CC	denotes	and
T20494	5841-5853	VB	denotes	precipitated
T20495	5854-5859	VB	denotes	using
T20496	5860-5871	NN	denotes	isopropanol
T20497	5873-5882	VB	denotes	Protected
T20498	5883-5889	NN	denotes	probes
T20499	5890-5894	VB	denotes	were
T20500	5895-5903	VB	denotes	resolved
T20501	5904-5906	IN	denotes	on
T20502	5907-5908	DT	denotes	a
T20503	5909-5919	VB	denotes	denaturing
T20504	5920-5924	CD	denotes	4.75
T20505	5924-5925	NN	denotes	%
T20506	5926-5936	NN	denotes	acrylamide
T20507	5937-5940	NN	denotes	gel
T20508	5940-5941	-COMMA-	denotes	,
T20509	5942-5947	VB	denotes	dried
T20510	5947-5948	-COMMA-	denotes	,
T20511	5949-5952	CC	denotes	and
T20512	5953-5959	VB	denotes	imaged
T20513	5960-5965	VB	denotes	using
T20514	5966-5967	DT	denotes	a
T20515	5968-5982	NN	denotes	phosphorImager
T20516	5983-5984	-LRB-	denotes	(
T20517	5984-5993	NNP	denotes	Molecular
T20518	5994-6002	NNP	denotes	Dynamics
T20519	6002-6003	-RRB-	denotes	)
T20520	6005-6017	NN	denotes	Densitometry
T20521	6018-6021	VB	denotes	was
T20522	6022-6031	VB	denotes	performed
T20523	6032-6037	VB	denotes	using
T20524	6038-6041	DT	denotes	the
T20525	6042-6052	JJ	denotes	ImageQuant
T20526	6053-6055	NN	denotes	TL
T20527	6056-6061	NN	denotes	v2005
T20528	6062-6070	NN	denotes	software
T20529	6071-6072	-LRB-	denotes	(
T20530	6072-6074	NNP	denotes	GE
T20531	6075-6085	NNP	denotes	Healthcare
T20532	6085-6086	-RRB-	denotes	)
T20773	6089-6092	NN	denotes	PCR
T20774	6093-6101	NN	denotes	analysis
T20775	6102-6104	IN	denotes	of
T20776	6105-6106	NN	denotes	κ
T20777	6107-6112	NN	denotes	chain
T20778	6113-6126	NN	denotes	rearrangement
T20779	6128-6131	NN	denotes	DNA
T20780	6132-6135	VB	denotes	was
T20781	6136-6144	VB	denotes	isolated
T20782	6145-6149	IN	denotes	from
T20783	6150-6161	NN	denotes	splenocytes
T20784	6162-6167	VB	denotes	using
T20785	6168-6171	DT	denotes	the
T20786	6172-6178	NNP	denotes	Dneasy
T20787	6179-6185	NN	denotes	Tissue
T20788	6186-6189	NN	denotes	Kit
T20789	6190-6191	-LRB-	denotes	(
T20790	6191-6197	NN	denotes	Qiagen
T20791	6197-6198	-RRB-	denotes	)
T20792	6198-6199	-COMMA-	denotes	,
T20793	6200-6204	IN	denotes	with
T20794	6205-6210	NN	denotes	Rnase
T20795	6211-6220	NN	denotes	treatment
T20796	6222-6225	NN	denotes	PCR
T20797	6226-6235	NN	denotes	reactions
T20798	6236-6245	VB	denotes	contained
T20799	6246-6249	CD	denotes	100
T20800	6250-6252	NN	denotes	ng
T20801	6253-6255	IN	denotes	of
T20802	6256-6263	JJ	denotes	genomic
T20803	6264-6267	NN	denotes	DNA
T20804	6267-6268	-COMMA-	denotes	,
T20805	6269-6272	CD	denotes	0.2
T20806	6273-6275	NN	denotes	μM
T20807	6276-6280	DT	denotes	each
T20808	6281-6287	NN	denotes	primer
T20809	6287-6288	-COMMA-	denotes	,
T20810	6289-6292	CD	denotes	0.2
T20811	6293-6295	NN	denotes	mM
T20812	6296-6301	NN	denotes	dNTPs
T20813	6301-6302	-COMMA-	denotes	,
T20814	6303-6306	CC	denotes	and
T20815	6307-6310	CD	denotes	2.5
T20816	6311-6312	NN	denotes	U
T20817	6313-6316	NN	denotes	Taq
T20818	6317-6320	NN	denotes	DNA
T20819	6321-6331	NN	denotes	polymerase
T20820	6332-6333	-LRB-	denotes	(
T20821	6333-6336	NN	denotes	NEB
T20822	6336-6337	-RRB-	denotes	)
T20823	6338-6347	VB	denotes	performed
T20824	6348-6350	IN	denotes	in
T20825	6351-6353	NN	denotes	1X
T20826	6354-6363	NN	denotes	ThermoPol
T20827	6364-6370	NN	denotes	buffer
T20828	6371-6372	-LRB-	denotes	(
T20829	6372-6375	NN	denotes	NEB
T20830	6375-6376	-RRB-	denotes	)
T20831	6378-6385	NN	denotes	Primers
T20832	6386-6390	VB	denotes	used
T20833	6391-6395	VB	denotes	were
T20834	6396-6401	NNP	denotes	Vκcon
T20835	6402-6405	CC	denotes	and
T20836	6406-6412	NN	denotes	Jκ5–1°
T20837	6413-6416	CC	denotes	and
T20838	6417-6421	VB	denotes	have
T20839	6422-6426	VB	denotes	been
T20840	6427-6436	VB	denotes	described
T20841	6437-6447	RB	denotes	previously
T20842	6448-6449	-LRB-	denotes	[
T20843	6449-6451	CD	denotes	74
T20844	6451-6452	-RRB-	denotes	]
T20845	6454-6457	NN	denotes	PCR
T20846	6458-6468	NN	denotes	conditions
T21034	6598-6608	NN	denotes	Immunoblot
T21035	6609-6617	NN	denotes	analysis
T21036	6619-6624	JJ	denotes	Whole
T21037	6625-6629	NN	denotes	cell
T21038	6630-6637	NN	denotes	lysates
T21039	6638-6642	VB	denotes	were
T21040	6643-6651	VB	denotes	prepared
T21041	6652-6654	IN	denotes	in
T21042	6655-6679	NN	denotes	radioimmunoprecipitation
T21043	6680-6685	NN	denotes	assay
T21044	6686-6687	-LRB-	denotes	(
T21045	6687-6691	NN	denotes	RIPA
T21046	6691-6692	-RRB-	denotes	)
T21047	6693-6699	NN	denotes	buffer
T21048	6700-6701	-LRB-	denotes	(
T21049	6701-6703	CD	denotes	20
T21050	6704-6706	NN	denotes	mM
T21051	6707-6715	NN	denotes	Tris-HCl
T21052	6716-6717	-LRB-	denotes	[
T21053	6717-6719	NN	denotes	pH
T21054	6720-6723	CD	denotes	7.5
T21055	6723-6724	-RRB-	denotes	]
T21056	6724-6725	-COMMA-	denotes	,
T21057	6726-6729	CD	denotes	150
T21058	6730-6732	NN	denotes	mM
T21059	6733-6737	NN	denotes	NaCl
T21060	6737-6738	-COMMA-	denotes	,
T21061	6739-6740	CD	denotes	1
T21062	6741-6743	NN	denotes	mM
T21063	6744-6748	NN	denotes	EDTA
T21064	6748-6749	-COMMA-	denotes	,
T21065	6750-6751	CD	denotes	1
T21066	6751-6752	NN	denotes	%
T21067	6753-6758	NN	denotes	NP-40
T21068	6758-6759	-COMMA-	denotes	,
T21069	6760-6763	CD	denotes	0.1
T21070	6763-6764	NN	denotes	%
T21071	6765-6768	NN	denotes	SDS
T21072	6768-6769	-COMMA-	denotes	,
T21073	6770-6773	CD	denotes	0.1
T21074	6773-6774	NN	denotes	%
T21075	6775-6781	NN	denotes	sodium
T21076	6782-6794	NN	denotes	deoxycholate
T21077	6794-6795	-RRB-	denotes	)
T21078	6796-6808	VB	denotes	supplemented
T21079	6809-6813	IN	denotes	with
T21080	6814-6815	CD	denotes	2
T21081	6816-6818	NN	denotes	mM
T21082	6819-6839	NN	denotes	phenylmethylsulfonyl
T21083	6840-6848	NN	denotes	fluoride
T21084	6848-6849	-COMMA-	denotes	,
T21085	6850-6851	CD	denotes	1
T21086	6852-6854	NN	denotes	mM
T21087	6855-6861	NN	denotes	Na3VO4
T21088	6861-6862	-COMMA-	denotes	,
T21089	6863-6866	CC	denotes	and
T21090	6867-6868	CD	denotes	1
T21091	6868-6869	SYM	denotes	:
T21092	6869-6872	CD	denotes	100
T21093	6873-6893	NN	denotes	protease/phosphatase
T21094	6894-6903	NN	denotes	inhibitor
T21095	6904-6913	NN	denotes	cocktails
T21096	6914-6915	-LRB-	denotes	(
T21097	6915-6920	NNP	denotes	Sigma
T21098	6920-6921	-RRB-	denotes	)
T21099	6923-6928	JJ	denotes	Crude
T21100	6929-6936	NN	denotes	lysates
T21101	6937-6941	VB	denotes	were
T21102	6942-6953	VB	denotes	centrifuged
T21103	6954-6956	IN	denotes	at
T21104	6957-6963	CD	denotes	13,000
T21105	6964-6967	NN	denotes	rpm
T21106	6968-6971	IN	denotes	for
T21107	6972-6974	CD	denotes	10
T21108	6975-6978	NN	denotes	min
T21109	6979-6981	IN	denotes	at
T21110	6982-6983	CD	denotes	4
T21111	6984-6986	NN	denotes	°C
T21112	6987-6990	CC	denotes	and
T21113	6991-6994	DT	denotes	the
T21114	6995-7007	NN	denotes	supernatants
T21115	7008-7012	VB	denotes	were
T21116	7013-7022	VB	denotes	collected
T21117	7023-7026	IN	denotes	for
T21118	7027-7034	JJ	denotes	further
T21119	7035-7043	NN	denotes	analysis
T21120	7045-7052	NN	denotes	Protein
T21121	7053-7067	NN	denotes	concentrations
T21122	7068-7072	VB	denotes	were
T21123	7073-7083	VB	denotes	determined
T21124	7084-7088	IN	denotes	with
T21125	7089-7092	DT	denotes	the
T21126	7093-7100	JJ	denotes	Bio-Rad
T21127	7101-7103	NN	denotes	DC
T21128	7104-7111	NN	denotes	protein
T21129	7112-7117	NN	denotes	assay
T21130	7118-7124	NN	denotes	system
T21131	7126-7133	NN	denotes	Lysates
T21132	7134-7138	VB	denotes	were
T21133	7139-7145	VB	denotes	boiled
T21134	7146-7148	IN	denotes	in
T21135	7149-7152	DT	denotes	the
T21136	7153-7161	NN	denotes	presence
T21137	7162-7164	IN	denotes	of
T21138	7165-7168	CD	denotes	2.5
T21139	7168-7169	NN	denotes	%
T21140	7170-7186	NN	denotes	β-mecaptoethanol
T21141	7186-7187	-COMMA-	denotes	,
T21142	7188-7197	VB	denotes	separated
T21143	7198-7200	IN	denotes	by
T21144	7201-7211	VB	denotes	denaturing
T21145	7212-7220	NN	denotes	SDS-PAGE
T21146	7220-7221	-COMMA-	denotes	,
T21147	7222-7225	CC	denotes	and
T21148	7226-7237	VB	denotes	transferred
T21149	7238-7240	TO	denotes	to
T21150	7241-7248	JJ	denotes	0.45-μm
T21151	7249-7257	NN	denotes	Optitran
T21152	7258-7267	NN	denotes	membranes
T21153	7268-7269	-LRB-	denotes	(
T21154	7269-7279	NNP	denotes	Schleicher
T21155	7280-7281	CC	denotes	&
T21156	7282-7289	NNP	denotes	Schuell
T21157	7289-7290	-RRB-	denotes	)
T21158	7291-7293	IN	denotes	in
T21159	7294-7295	DT	denotes	a
T21160	7296-7303	JJ	denotes	Bio-Rad
T21161	7304-7312	NN	denotes	transfer
T21162	7313-7317	NN	denotes	unit
T21163	7319-7328	NN	denotes	Membranes
T21164	7329-7333	VB	denotes	were
T21165	7334-7347	VB	denotes	immunoblotted
T21166	7348-7352	IN	denotes	with
T21167	7353-7356	DT	denotes	the
T21168	7357-7368	JJ	denotes	appropriate
T21169	7369-7376	JJ	denotes	primary
T21170	7377-7385	NN	denotes	antibody
T21171	7386-7394	VB	denotes	followed
T21172	7395-7397	IN	denotes	by
T21173	7398-7409	NN	denotes	horseradish
T21174	7410-7427	VB	denotes	peroxidase–tagged
T21175	7428-7437	JJ	denotes	secondary
T21176	7438-7448	NN	denotes	antibodies
T21177	7449-7450	-LRB-	denotes	(
T21178	7450-7458	NNP	denotes	Amersham
T21179	7459-7470	NNP	denotes	Biosciences
T21180	7471-7474	CC	denotes	and
T21181	7475-7479	NNP	denotes	Dako
T21182	7479-7480	-RRB-	denotes	)
T21183	7481-7484	CC	denotes	and
T21184	7485-7493	VB	denotes	detected
T21185	7494-7498	IN	denotes	with
T21186	7499-7502	DT	denotes	the
T21187	7503-7514	NNP	denotes	SuperSignal
T21188	7515-7519	NNP	denotes	West
T21189	7520-7524	NNP	denotes	Pico
T21190	7525-7531	NNP	denotes	System
T21191	7532-7533	-LRB-	denotes	(
T21192	7533-7539	NNP	denotes	Pierce
T21193	7539-7540	-RRB-	denotes	)
T20847	6469-6473	VB	denotes	were
T20848	6474-6476	CD	denotes	94
T20849	6477-6479	NN	denotes	°C
T20850	6480-6483	IN	denotes	for
T20851	6484-6485	CD	denotes	2
T20852	6486-6489	NN	denotes	min
T20853	6489-6490	-COMMA-	denotes	,
T20854	6491-6493	CD	denotes	40
T20855	6494-6500	NN	denotes	cycles
T20856	6501-6503	IN	denotes	of
T20857	6504-6506	CD	denotes	94
T20858	6507-6509	NN	denotes	°C
T20859	6510-6513	IN	denotes	for
T20860	6514-6516	CD	denotes	30
T20861	6517-6518	NN	denotes	s
T20862	6518-6519	-COMMA-	denotes	,
T20863	6520-6522	CD	denotes	63
T20864	6523-6525	NN	denotes	°C
T20865	6526-6529	IN	denotes	for
T20866	6530-6532	CD	denotes	90
T20867	6533-6534	NN	denotes	s
T20868	6534-6535	-COMMA-	denotes	,
T20869	6536-6539	CC	denotes	and
T20870	6540-6542	CD	denotes	72
T20871	6543-6545	NN	denotes	°C
T20872	6546-6549	IN	denotes	for
T20873	6550-6551	CD	denotes	1
T20874	6552-6555	NN	denotes	min
T20875	6555-6556	-COMMA-	denotes	,
T20876	6557-6565	VB	denotes	followed
T20877	6566-6568	IN	denotes	by
T20878	6569-6570	CD	denotes	1
T20879	6571-6576	NN	denotes	cycle
T20880	6577-6579	IN	denotes	of
T20881	6580-6582	CD	denotes	72
T20882	6583-6585	NN	denotes	°C
T20883	6586-6589	IN	denotes	for
T20884	6590-6591	CD	denotes	5
T20885	6592-6595	NN	denotes	min
R14045	T18296	T18295	arg1Of	mice,Transgenic
R14046	T18297	T18298	arg1Of	Generation,of
R14047	T18297	T18301	arg1Of	Generation,under
R14048	T18297	T18309	arg1Of	Generation,have
R14049	T18297	T18310	arg1Of	Generation,been
R14050	T18297	T18311	arg2Of	Generation,described
R14051	T18297	T18314	arg1Of	Generation,were
R14052	T18297	T18315	arg2Of	Generation,maintained
R14053	T18300	T18298	arg2Of	mice,of
R14054	T18300	T18299	arg1Of	mice,LMP1
R14055	T18306	T18302	arg1Of	promoter,the
R14056	T18306	T18303	arg1Of	promoter,Ig
R14057	T18306	T18304	arg1Of	promoter,heavy
R14058	T18306	T18305	arg1Of	promoter,chain
R14059	T18306	T18307	arg1Of	promoter,and
R14060	T18307	T18301	arg2Of	and,under
R14061	T18308	T18307	arg2Of	enhancer,and
R14062	T18311	T18309	arg2Of	described,have
R14063	T18311	T18310	arg2Of	described,been
R14064	T18311	T18312	arg1Of	described,previously
R14065	T18311	T18313	arg1Of	described,and
R14066	T18313	T18322	arg1Of	and,[
R14067	T18315	T18313	arg2Of	maintained,and
R14068	T18315	T18314	arg2Of	maintained,were
R14069	T18315	T18316	arg1Of	maintained,as
R14070	T18315	T18318	arg1Of	maintained,on
R14071	T18317	T18316	arg2Of	heterozygotes,as
R14072	T18321	T18318	arg2Of	background,on
R14073	T18321	T18319	arg1Of	background,a
R14074	T18321	T18320	arg1Of	background,Balbc
R14075	T18323	T18322	arg2Of	26,[
R14076	T18324	T18322	arg3Of	],[
R14077	T18326	T18325	arg1Of	mice,LMP1
R14078	T18326	T18327	arg1Of	mice,were
R14079	T18326	T18328	arg2Of	mice,genotyped
R14080	T18328	T18327	arg2Of	genotyped,were
R14081	T18328	T18338	arg1Of	genotyped,as
R14082	T18331	T18332	arg1Of	blot,and
R14083	T18333	T18332	arg2Of	PCR,and
R14084	T18334	T18328	arg1Of	analysis,genotyped
R14085	T18334	T18329	arg2Of	analysis,by
R14086	T18334	T18330	arg1Of	analysis,Southern
R14087	T18334	T18331	arg1Of	analysis,blot
R14088	T18334	T18333	arg1Of	analysis,PCR
R14089	T18334	T18335	arg1Of	analysis,of
R14090	T18337	T18335	arg2Of	DNA,of
R14091	T18337	T18336	arg1Of	DNA,tail
R14092	T18339	T18338	arg2Of	described,as
R14093	T18339	T18340	arg1Of	described,previously
R14094	T18339	T18341	arg1Of	described,[
R14095	T18342	T18341	arg2Of	26,[
R14096	T18343	T18341	arg3Of	],[
R14097	T18344	T18345	arg1Of	Spleen,and
R14098	T18346	T18345	arg2Of	liver,and
R14099	T18347	T18344	arg1Of	sections,Spleen
R14100	T18347	T18346	arg1Of	sections,liver
R14101	T18347	T18348	arg1Of	sections,were
R14102	T18347	T18349	arg2Of	sections,fixed
R14103	T18347	T18355	arg2Of	sections,embedded
R14104	T18349	T18350	arg1Of	fixed,in
R14105	T18349	T18354	arg1Of	fixed,and
R14106	T18351	T18352	arg1Of	4,%
R14107	T18353	T18350	arg2Of	paraformaldehyde,in
R14108	T18353	T18351	arg1Of	paraformaldehyde,4
R14109	T18354	T18348	arg2Of	and,were
R14110	T18354	T18359	arg1Of	and,and
R14111	T18355	T18354	arg2Of	embedded,and
R14112	T18355	T18356	arg1Of	embedded,in
R14113	T18357	T18356	arg2Of	paraffin,in
R14114	T18359	T18358	arg1Of	and,","
R14115	T18361	T18360	arg1Of	sections,5-μm
R14116	T18361	T18362	arg1Of	sections,were
R14117	T18361	T18363	arg2Of	sections,stained
R14118	T18363	T18359	arg2Of	stained,and
R14119	T18363	T18362	arg2Of	stained,were
R14120	T18363	T18364	arg1Of	stained,with
R14121	T18365	T18366	arg1Of	hematoxylin,and
R14122	T18366	T18364	arg2Of	and,with
R14123	T18366	T18368	arg1Of	and,for
R14124	T18367	T18366	arg2Of	eosin,and
R14125	T18370	T18368	arg2Of	analysis,for
R14126	T18370	T18369	arg1Of	analysis,histopathological
R14127	T18371	T18372	arg1Of	Lymphomas,were
R14128	T18371	T18373	arg2Of	Lymphomas,passaged
R14129	T18371	T18386	arg2Of	Lymphomas,sacrificed
R14130	T18373	T18382	arg1Of	passaged,into
R14131	T18373	T18385	arg1Of	passaged,and
R14132	T18376	T18373	arg1Of	injection,passaged
R14133	T18376	T18374	arg2Of	injection,by
R14134	T18376	T18375	arg1Of	injection,intraperitoneal
R14135	T18376	T18377	arg1Of	injection,of
R14136	T18381	T18377	arg2Of	splenocytes,of
R14137	T18381	T18378	arg1Of	splenocytes,1
R14138	T18381	T18379	arg1Of	splenocytes,×
R14139	T18381	T18380	arg1Of	splenocytes,108
R14140	T18384	T18382	arg2Of	mice,into
R14141	T18384	T18383	arg1Of	mice,SCID
R14142	T18385	T18372	arg2Of	and,were
R14143	T18386	T18385	arg2Of	sacrificed,and
R14144	T18386	T18387	arg1Of	sacrificed,upon
R14145	T18388	T18387	arg2Of	development,upon
R14146	T18388	T18389	arg1Of	development,of
R14147	T18392	T18389	arg2Of	abdomen,of
R14148	T18392	T18390	arg1Of	abdomen,an
R14149	T18392	T18391	arg2Of	abdomen,extended
R14150	T18393	T18394	arg1Of	Animals,were
R14151	T18393	T18395	arg2Of	Animals,housed
R14152	T18395	T18394	arg2Of	housed,were
R14153	T18395	T18396	arg1Of	housed,in
R14154	T18398	T18396	arg2Of	Association,in
R14155	T18398	T18397	arg1Of	Association,the
R14156	T18398	T18399	arg1Of	Association,for
R14157	T18398	T18403	arg1Of	Association,for
R14158	T18400	T18401	arg1Of	Assessment,and
R14159	T18401	T18399	arg2Of	and,for
R14160	T18402	T18401	arg2Of	Accreditation,and
R14161	T18405	T18404	arg1Of	Care–approved,Animal
R14162	T18407	T18403	arg2Of	facility,for
R14163	T18407	T18405	arg1Of	facility,Care–approved
R14164	T18407	T18406	arg1Of	facility,animal
R14165	T18407	T18408	arg1Of	facility,at
R14166	T18407	T18414	arg1Of	facility,at
R14167	T18407	T18419	modOf	facility,were
R14168	T18410	T18408	arg2Of	University,at
R14169	T18410	T18409	arg1Of	University,the
R14170	T18410	T18411	arg1Of	University,of
R14171	T18413	T18411	arg2Of	Carolina,of
R14172	T18413	T18412	arg1Of	Carolina,North
R14173	T18416	T18414	arg2Of	Hill.,at
R14174	T18416	T18415	arg1Of	Hill.,Chapel
R14175	T18418	T18417	arg1Of	protocols,All
R14176	T18418	T18419	arg1Of	protocols,were
R14177	T18418	T18420	arg2Of	protocols,approved
R14178	T18420	T18419	arg2Of	approved,were
R14179	T18425	T18423	arg1Of	Care,Institutional
R14180	T18425	T18424	arg1Of	Care,Animal
R14181	T18425	T18426	arg1Of	Care,and
R14182	T18426	T18420	arg1Of	and,approved
R14183	T18426	T18421	arg2Of	and,by
R14184	T18426	T18422	arg1Of	and,the
R14185	T18428	T18426	arg2Of	Committee,and
R14186	T18428	T18427	arg1Of	Committee,Use
R14329	T18617	T18618	arg1Of	Isolation,and
R14330	T18618	T18620	arg1Of	and,of
R14331	T18619	T18618	arg2Of	growth,and
R14332	T18622	T18620	arg2Of	cells,of
R14333	T18622	T18621	arg1Of	cells,B
R14334	T18623	T18624	arg1Of	Splenocytes,were
R14335	T18623	T18625	arg2Of	Splenocytes,prepared
R14336	T18625	T18624	arg2Of	prepared,were
R14337	T18625	T18626	arg1Of	prepared,by
R14338	T18625	T18634	arg1Of	prepared,and
R14339	T18627	T18626	arg2Of	homogenizing,by
R14340	T18627	T18630	arg1Of	homogenizing,with
R14341	T18629	T18627	arg2Of	tissue,homogenizing
R14342	T18629	T18628	arg1Of	tissue,spleen
R14343	T18633	T18630	arg2Of	slides,with
R14344	T18633	T18631	arg1Of	slides,two
R14345	T18633	T18632	arg1Of	slides,frosted
R14346	T18635	T18636	arg1Of	debris,was
R14347	T18635	T18637	arg2Of	debris,filtered
R14348	T18637	T18634	arg2Of	filtered,and
R14349	T18637	T18636	arg2Of	filtered,was
R14350	T18637	T18638	arg1Of	filtered,through
R14351	T18642	T18638	arg2Of	strainer,through
R14352	T18642	T18639	arg1Of	strainer,a
R14353	T18642	T18640	arg1Of	strainer,100-μm
R14354	T18642	T18641	arg1Of	strainer,cell
R14355	T18643	T18644	arg1Of	Erythrocytes,were
R14356	T18643	T18645	arg2Of	Erythrocytes,lysed
R14357	T18643	T18662	arg2Of	Erythrocytes,washed
R14358	T18645	T18661	arg1Of	lysed,and
R14359	T18646	T18645	arg3Of	using,lysed
R14360	T18647	T18648	arg1Of	0.8,%
R14361	T18651	T18646	arg2Of	solution,using
R14362	T18651	T18647	arg1Of	solution,0.8
R14363	T18651	T18649	arg1Of	solution,ammonium
R14364	T18651	T18650	arg1Of	solution,chloride
R14365	T18651	T18652	arg1Of	solution,(
R14366	T18651	T18656	arg1Of	solution,for
R14367	T18651	T18659	arg1Of	solution,on
R14368	T18654	T18652	arg2Of	Technologies,(
R14369	T18654	T18653	arg1Of	Technologies,StemCell
R14370	T18655	T18652	arg3Of	),(
R14371	T18658	T18656	arg2Of	min,for
R14372	T18658	T18657	arg1Of	min,10
R14373	T18660	T18659	arg2Of	ice,on
R14374	T18661	T18644	arg2Of	and,were
R14375	T18662	T18661	arg2Of	washed,and
R14376	T18662	T18663	arg1Of	washed,twice
R14377	T18662	T18664	arg1Of	washed,with
R14378	T18665	T18664	arg2Of	PBS,with
R14379	T18667	T18666	arg1Of	cells,B
R14380	T18667	T18668	arg1Of	cells,were
R14381	T18667	T18669	arg2Of	cells,isolated
R14382	T18667	T18684	arg2Of	cells,grown
R14383	T18669	T18670	modOf	isolated,using
R14384	T18669	T18673	arg1Of	isolated,according
R14385	T18669	T18683	arg1Of	isolated,and
R14386	T18672	T18670	arg2Of	beads,using
R14387	T18672	T18671	arg1Of	beads,CD19-MACS
R14388	T18674	T18673	arg2Of	to,according
R14389	T18676	T18675	arg1Of	manufacturer,the
R14390	T18676	T18677	arg2Of	manufacturer,'s
R14391	T18678	T18674	arg2Of	instructions,to
R14392	T18678	T18677	arg1Of	instructions,'s
R14393	T18678	T18679	arg1Of	instructions,(
R14394	T18681	T18679	arg2Of	Biotec,(
R14395	T18681	T18680	arg1Of	Biotec,Miltenyi
R14396	T18682	T18679	arg3Of	),(
R14397	T18683	T18668	arg2Of	and,were
R14398	T18684	T18683	arg2Of	grown,and
R14399	T18684	T18685	arg1Of	grown,in
R14400	T18686	T18687	arg2Of	Iscove,'s
R14401	T18688	T18685	arg2Of	medium,in
R14402	T18688	T18687	arg1Of	medium,'s
R14403	T18688	T18689	arg2Of	medium,supplemented
R14404	T18689	T18690	arg1Of	supplemented,with
R14405	T18692	T18693	arg1Of	10,%
R14406	T18696	T18691	arg1Of	serum,heat-inactivated
R14407	T18696	T18692	arg1Of	serum,10
R14408	T18696	T18694	arg1Of	serum,fetal
R14409	T18696	T18695	arg1Of	serum,bovine
R14410	T18696	T18697	arg1Of	serum,and
R14411	T18697	T18690	arg2Of	and,with
R14412	T18698	T18697	arg2Of	antibiotic/antimycotic,and
R14413	T18698	T18699	arg1Of	antibiotic/antimycotic,(
R14414	T18700	T18699	arg2Of	GIBCO,(
R14415	T18701	T18699	arg3Of	),(
R14416	T18702	T18703	arg2Of	Splenocytes,isolated
R14417	T18702	T18707	arg1Of	Splenocytes,consisted
R14418	T18702	T18722	arg1Of	Splenocytes,were
R14419	T18702	T18726	arg2Of	Splenocytes,purified
R14420	T18703	T18704	arg1Of	isolated,from
R14421	T18706	T18704	arg2Of	lymphomas,from
R14422	T18706	T18705	arg1Of	lymphomas,SCID-passaged
R14423	T18707	T18708	arg1Of	consisted,of
R14424	T18707	T18715	arg1Of	consisted,as
R14425	T18707	T18721	arg1Of	consisted,and
R14426	T18711	T18712	arg1Of	–90,%
R14427	T18714	T18708	arg2Of	cells,of
R14428	T18714	T18709	arg1Of	cells,80
R14429	T18714	T18710	arg1Of	cells,%
R14430	T18714	T18711	arg1Of	cells,–90
R14431	T18714	T18713	arg1Of	cells,B
R14432	T18716	T18715	arg2Of	determined,as
R14433	T18719	T18716	arg1Of	cytometry,determined
R14434	T18719	T18717	arg2Of	cytometry,by
R14435	T18719	T18718	arg1Of	cytometry,flow
R14436	T18721	T18720	arg1Of	and,","
R14437	T18725	T18724	arg1Of	further,not
R14438	T18726	T18721	arg2Of	purified,and
R14439	T18726	T18722	arg2Of	purified,were
R14440	T18726	T18723	arg1Of	purified,hence
R14441	T18726	T18725	arg1Of	purified,further
R14442	T18726	T18727	arg1Of	purified,with
R14443	T18729	T18727	arg2Of	beads,with
R14444	T18729	T18728	arg1Of	beads,CD19-MACS
R14445	T18730	T18731	arg1Of	Splenocytes,were
R14446	T18730	T18732	arg2Of	Splenocytes,seeded
R14447	T18732	T18731	arg2Of	seeded,were
R14448	T18732	T18733	arg1Of	seeded,at
R14449	T18734	T18735	arg1Of	1.25,×
R14450	T18736	T18735	arg2Of	106,×
R14451	T18737	T18734	arg1Of	cells/ml,1.25
R14452	T18737	T18736	arg1Of	cells/ml,106
R14453	T18737	T18738	arg1Of	cells/ml,and
R14454	T18738	T18733	arg2Of	and,at
R14455	T18739	T18738	arg2Of	where,and
R14456	T18740	T18741	arg1Of	applicable,","
R14457	T18742	T18741	arg2Of	recombinant,","
R14458	T18744	T18740	arg1Of	IL4,applicable
R14459	T18744	T18742	arg1Of	IL4,recombinant
R14460	T18744	T18743	arg1Of	IL4,mouse
R14461	T18744	T18745	arg1Of	IL4,was
R14462	T18744	T18746	arg2Of	IL4,added
R14463	T18746	T18739	arg1Of	added,where
R14464	T18746	T18745	arg2Of	added,was
R14465	T18746	T18747	arg1Of	added,at
R14466	T18746	T18771	arg1Of	added,(
R14467	T18749	T18748	arg1Of	ng/ml,100
R14468	T18749	T18750	arg1Of	ng/ml,","
R14469	T18750	T18758	arg1Of	",",and
R14470	T18753	T18750	arg2Of	IL10,","
R14471	T18753	T18751	arg1Of	IL10,recombinant
R14472	T18753	T18752	arg1Of	IL10,mouse
R14473	T18753	T18754	arg1Of	IL10,at
R14474	T18756	T18754	arg2Of	ng/ml,at
R14475	T18756	T18755	arg1Of	ng/ml,10
R14476	T18758	T18747	arg2Of	and,at
R14477	T18758	T18757	arg1Of	and,","
R14478	T18762	T18759	arg1Of	IL10,rat
R14479	T18762	T18760	arg1Of	IL10,IgG1
R14480	T18762	T18761	arg1Of	IL10,anti-mouse
R14481	T18762	T18763	arg1Of	IL10,and
R14482	T18763	T18758	arg2Of	and,and
R14483	T18763	T18768	arg1Of	and,at
R14484	T18767	T18763	arg2Of	control,and
R14485	T18767	T18764	arg1Of	control,rat
R14486	T18767	T18765	arg1Of	control,IgG1
R14487	T18767	T18766	arg1Of	control,isotype
R14488	T18770	T18768	arg2Of	μg/ml,at
R14489	T18770	T18769	arg1Of	μg/ml,10
R14490	T18775	T18771	arg2Of	Systems,(
R14491	T18775	T18772	arg1Of	Systems,R
R14492	T18775	T18773	arg1Of	Systems,&
R14493	T18775	T18774	arg1Of	Systems,D
R14494	T18776	T18771	arg3Of	),(
R14495	T18780	T18777	arg2Of	assays,For
R14496	T18780	T18778	arg1Of	assays,BrdU
R14497	T18780	T18779	arg1Of	assays,incorporation
R14498	T18782	T18783	arg1Of	splenocytes,were
R14499	T18782	T18784	arg2Of	splenocytes,pulsed
R14500	T18784	T18777	arg1Of	pulsed,For
R14501	T18784	T18781	arg1Of	pulsed,","
R14502	T18784	T18783	arg2Of	pulsed,were
R14503	T18784	T18785	arg1Of	pulsed,for
R14504	T18784	T18788	arg1Of	pulsed,with
R14505	T18787	T18785	arg2Of	h,for
R14506	T18787	T18786	arg1Of	h,24
R14507	T18791	T18790	arg1Of	BrdU,μM
R14508	T18791	T18792	arg1Of	BrdU,1
R14509	T18793	T18788	arg2Of	d,with
R14510	T18793	T18789	arg1Of	d,10
R14511	T18793	T18791	arg1Of	d,BrdU
R14512	T18793	T18794	arg1Of	d,post-harvest
R14700	T19052	T19051	arg1Of	assay,MTS
R14701	T19054	T19053	arg1Of	3-,The
R14702	T19054	T19055	arg1Of	3-,(
R14703	T19054	T19060	arg1Of	3-,-5-
R14704	T19054	T19061	arg1Of	3-,(
R14705	T19054	T19064	arg1Of	3-,-2-
R14706	T19054	T19077	modOf	3-,were
R14707	T19056	T19055	arg2Of	4,(
R14708	T19056	T19057	arg1Of	4,","
R14709	T19058	T19057	arg2Of	5-dimethylthiazol-2-yl,","
R14710	T19059	T19055	arg3Of	),(
R14711	T19062	T19061	arg2Of	3-carboxymethoxyphenyl,(
R14712	T19063	T19061	arg3Of	),(
R14713	T19066	T19065	arg2Of	4-sulfophenyl,(
R14714	T19067	T19065	arg3Of	),(
R14715	T19072	T19071	arg2Of	MTS,(
R14716	T19073	T19071	arg3Of	),(
R14717	T19076	T19065	arg1Of	assays,(
R14718	T19076	T19068	arg1Of	assays,-2H-tetrazolium
R14719	T19076	T19069	arg1Of	assays,inner
R14720	T19076	T19070	arg1Of	assays,salt
R14721	T19076	T19071	arg1Of	assays,(
R14722	T19076	T19074	arg1Of	assays,cell
R14723	T19076	T19075	arg1Of	assays,cytotoxicity/proliferation
R14724	T19076	T19077	arg1Of	assays,were
R14725	T19076	T19078	arg2Of	assays,performed
R14726	T19078	T19077	arg2Of	performed,were
R14727	T19078	T19091	arg1Of	performed,","
R14728	T19078	T19092	arg1Of	performed,according
R14729	T19079	T19078	arg3Of	using,performed
R14730	T19081	T19082	arg1Of	CellTiter,96
R14731	T19087	T19079	arg2Of	assay,using
R14732	T19087	T19080	arg1Of	assay,the
R14733	T19087	T19081	arg1Of	assay,CellTiter
R14734	T19087	T19083	arg1Of	assay,aqueous
R14735	T19087	T19084	arg1Of	assay,one-solution
R14736	T19087	T19085	arg1Of	assay,cell
R14737	T19087	T19086	arg1Of	assay,proliferation
R14738	T19087	T19088	arg1Of	assay,(
R14739	T19089	T19088	arg2Of	Promega,(
R14740	T19090	T19088	arg3Of	),(
R14741	T19093	T19092	arg2Of	to,according
R14742	T19094	T19095	arg2Of	manufacturer,'s
R14743	T19096	T19093	arg2Of	instructions,to
R14744	T19096	T19095	arg1Of	instructions,'s
R14745	T19099	T19097	arg2Of	studies,For
R14746	T19099	T19098	arg1Of	studies,IL4
R14747	T19101	T19102	arg1Of	splenocytes,were
R14748	T19101	T19103	arg2Of	splenocytes,cultured
R14749	T19103	T19097	arg1Of	cultured,For
R14750	T19103	T19100	arg1Of	cultured,","
R14751	T19103	T19102	arg2Of	cultured,were
R14752	T19103	T19104	arg1Of	cultured,for
R14753	T19103	T19107	arg1Of	cultured,in
R14754	T19106	T19104	arg2Of	d,for
R14755	T19106	T19105	arg1Of	d,3
R14756	T19109	T19110	arg1Of	presence,or
R14757	T19110	T19107	arg2Of	or,in
R14758	T19110	T19108	arg1Of	or,the
R14759	T19110	T19112	arg1Of	or,of
R14760	T19111	T19110	arg2Of	absence,or
R14761	T19113	T19114	arg1Of	100,ng/ml
R14762	T19115	T19112	arg2Of	IL4,of
R14763	T19115	T19113	arg1Of	IL4,100
R14764	T19116	T19117	arg1Of	Cells,were
R14765	T19116	T19118	arg2Of	Cells,seeded
R14766	T19118	T19117	arg2Of	seeded,were
R14767	T19118	T19119	arg1Of	seeded,on
R14768	T19118	T19122	arg1Of	seeded,in
R14769	T19118	T19124	arg1Of	seeded,in
R14770	T19120	T19119	arg2Of	day,on
R14771	T19120	T19121	arg1Of	day,3
R14772	T19123	T19122	arg2Of	triplicate,in
R14773	T19127	T19124	arg2Of	plate,in
R14774	T19127	T19125	arg1Of	plate,a
R14775	T19127	T19126	arg1Of	plate,96-well
R14776	T19127	T19128	arg1Of	plate,at
R14777	T19127	T19133	arg1Of	plate,at
R14778	T19129	T19130	arg1Of	2.5,×
R14779	T19131	T19130	arg2Of	106,×
R14780	T19132	T19128	arg2Of	cells/ml,at
R14781	T19132	T19129	arg1Of	cells/ml,2.5
R14782	T19132	T19131	arg1Of	cells/ml,106
R14783	T19135	T19133	arg2Of	μl,at
R14784	T19135	T19134	arg1Of	μl,100
R14785	T19135	T19136	arg1Of	μl,per
R14786	T19137	T19136	arg2Of	well,per
R14787	T19139	T19138	arg1Of	reagent,MTS
R14788	T19139	T19140	arg1Of	reagent,was
R14789	T19139	T19141	arg2Of	reagent,added
R14790	T19141	T19140	arg2Of	added,was
R14791	T19141	T19142	arg1Of	added,for
R14792	T19141	T19145	arg1Of	added,and
R14793	T19144	T19142	arg2Of	h,for
R14794	T19144	T19143	arg1Of	h,4
R14795	T19145	T19152	arg1Of	and,;
R14796	T19146	T19147	arg1Of	absorbance,was
R14797	T19146	T19148	arg2Of	absorbance,read
R14798	T19148	T19145	arg2Of	read,and
R14799	T19148	T19147	arg2Of	read,was
R14800	T19148	T19149	arg1Of	read,at
R14801	T19151	T19149	arg2Of	nm,at
R14802	T19151	T19150	arg1Of	nm,540
R14803	T19153	T19154	arg2Of	values,plotted
R14804	T19153	T19155	arg1Of	values,were
R14805	T19153	T19156	arg2Of	values,subtracted
R14806	T19156	T19152	arg2Of	subtracted,;
R14807	T19156	T19155	arg2Of	subtracted,were
R14808	T19156	T19157	arg1Of	subtracted,from
R14809	T19158	T19157	arg2Of	blanks,from
R14810	T19161	T19159	arg2Of	assays,For
R14811	T19161	T19160	arg1Of	assays,neutralization
R14812	T19163	T19164	arg1Of	splenocytes,were
R14813	T19163	T19165	arg2Of	splenocytes,seeded
R14814	T19165	T19159	arg1Of	seeded,For
R14815	T19165	T19162	arg1Of	seeded,","
R14816	T19165	T19164	arg2Of	seeded,were
R14817	T19165	T19166	arg1Of	seeded,at
R14818	T19165	T19170	arg1Of	seeded,cells/ml
R14819	T19167	T19166	arg2Of	5,at
R14820	T19170	T19168	arg1Of	cells/ml,×
R14821	T19170	T19169	arg1Of	cells/ml,106
R14822	T19170	T19171	arg1Of	cells/ml,at
R14823	T19170	T19199	modOf	cells/ml,were
R14824	T19173	T19171	arg2Of	μl,at
R14825	T19173	T19172	arg1Of	μl,100
R14826	T19177	T19174	arg1Of	day,per
R14827	T19177	T19175	arg1Of	day,well
R14828	T19177	T19176	arg1Of	day,on
R14829	T19177	T19178	arg1Of	day,of
R14830	T19177	T19199	arg1Of	day,were
R14831	T19177	T19200	arg2Of	day,added
R14832	T19179	T19180	arg1Of	harvest,and
R14833	T19180	T19182	arg1Of	and,","
R14834	T19181	T19180	arg2Of	IL4,and
R14835	T19182	T19188	arg1Of	",",or
R14836	T19186	T19182	arg2Of	IL4,","
R14837	T19186	T19183	arg1Of	IL4,rat
R14838	T19186	T19184	arg1Of	IL4,IgG1
R14839	T19186	T19185	arg1Of	IL4,anti-mouse
R14840	T19188	T19178	arg2Of	or,of
R14841	T19188	T19187	arg1Of	or,","
R14842	T19192	T19188	arg2Of	control,or
R14843	T19192	T19189	arg1Of	control,rat
R14844	T19192	T19190	arg1Of	control,IgG1
R14845	T19192	T19191	arg1Of	control,isotype
R14846	T19192	T19193	arg1Of	control,(
R14847	T19197	T19193	arg2Of	Systems,(
R14848	T19197	T19194	arg1Of	Systems,R
R14849	T19197	T19195	arg1Of	Systems,&
R14850	T19197	T19196	arg1Of	Systems,D
R14851	T19198	T19193	arg3Of	),(
R14852	T19200	T19199	arg2Of	added,were
R14853	T19200	T19201	arg1Of	added,at
R14854	T19203	T19201	arg2Of	concentrations,at
R14855	T19203	T19202	arg1Of	concentrations,the
R14856	T19203	T19204	arg2Of	concentrations,indicated
R14857	T19204	T19205	arg1Of	indicated,in
R14858	T19207	T19205	arg2Of	figures,in
R14859	T19207	T19206	arg1Of	figures,the
R14860	T19208	T19209	arg1Of	Cultures,were
R14861	T19208	T19210	arg2Of	Cultures,pulsed
R14862	T19210	T19209	arg2Of	pulsed,were
R14863	T19210	T19211	arg1Of	pulsed,for
R14864	T19213	T19211	arg2Of	h,for
R14865	T19213	T19212	arg1Of	h,4
R14866	T19213	T19214	arg1Of	h,with
R14867	T19218	T19214	arg2Of	d,with
R14868	T19218	T19215	arg1Of	d,MTS
R14869	T19218	T19216	arg1Of	d,reagent
R14870	T19218	T19217	arg1Of	d,1
R14871	T19218	T19219	arg1Of	d,post-seeding
R14872	T19222	T19220	arg2Of	studies,For
R14873	T19222	T19221	arg1Of	studies,inhibitor
R14874	T19224	T19225	arg1Of	splenocytes,were
R14875	T19224	T19226	arg2Of	splenocytes,seeded
R14876	T19226	T19220	arg1Of	seeded,For
R14877	T19226	T19223	arg1Of	seeded,","
R14878	T19226	T19225	arg2Of	seeded,were
R14879	T19226	T19227	arg1Of	seeded,at
R14880	T19226	T19232	arg1Of	seeded,at
R14881	T19226	T19242	arg1Of	seeded,and
R14882	T19231	T19227	arg2Of	cells/ml,at
R14883	T19231	T19228	arg1Of	cells/ml,1
R14884	T19231	T19229	arg1Of	cells/ml,×
R14885	T19231	T19230	arg1Of	cells/ml,107
R14886	T19234	T19233	arg1Of	μl,100
R14887	T19235	T19234	arg1Of	per,μl
R14888	T19238	T19232	arg2Of	day,at
R14889	T19238	T19235	arg1Of	day,per
R14890	T19238	T19236	arg1Of	day,well
R14891	T19238	T19237	arg1Of	day,on
R14892	T19238	T19239	arg1Of	day,of
R14893	T19240	T19239	arg2Of	harvest,of
R14894	T19242	T19241	arg1Of	and,","
R14895	T19243	T19244	arg1Of	inhibitors,were
R14896	T19243	T19245	arg2Of	inhibitors,added
R14897	T19245	T19242	arg2Of	added,and
R14898	T19245	T19244	arg2Of	added,were
R14899	T19245	T19246	arg1Of	added,at
R14900	T19248	T19246	arg2Of	concentrations,at
R14901	T19248	T19247	arg1Of	concentrations,the
R14902	T19248	T19249	arg2Of	concentrations,indicated
R14903	T19249	T19250	arg1Of	indicated,in
R14904	T19252	T19250	arg2Of	figures,in
R14905	T19252	T19251	arg1Of	figures,the
R14906	T19255	T19256	arg1Of	BAY11–7085,","
R14907	T19256	T19258	arg1Of	",",","
R14908	T19257	T19256	arg2Of	rapamycin,","
R14909	T19258	T19260	arg1Of	",",","
R14910	T19259	T19258	arg2Of	triciribine,","
R14911	T19260	T19262	arg1Of	",",","
R14912	T19261	T19260	arg2Of	U0126,","
R14913	T19262	T19254	arg1Of	",",inhibitors
R14914	T19262	T19265	arg1Of	",",and
R14915	T19263	T19262	arg2Of	SB202190,","
R14916	T19265	T19264	arg1Of	and,","
R14917	T19265	T19277	arg1Of	and,","
R14918	T19266	T19265	arg2Of	cucurbitacin,and
R14919	T19266	T19268	modOf	cucurbitacin,were
R14920	T19267	T19268	arg1Of	I,were
R14921	T19267	T19269	arg2Of	I,purchased
R14922	T19269	T19268	arg2Of	purchased,were
R14923	T19269	T19270	arg1Of	purchased,from
R14924	T19269	T19273	arg1Of	purchased,For
R14925	T19272	T19270	arg2Of	Biosciences.,from
R14926	T19272	T19271	arg1Of	Biosciences.,EMD
R14927	T19276	T19273	arg2Of	cultures,For
R14928	T19276	T19274	arg1Of	cultures,non-malignant
R14929	T19276	T19275	arg1Of	cultures,splenocyte
R14930	T19277	T19253	arg1Of	",",The
R14931	T19277	T19281	arg1Of	",",was
R14932	T19277	T19282	arg2Of	",",induced
R14933	T19280	T19277	arg2Of	activation,","
R14934	T19280	T19278	arg1Of	activation,B
R14935	T19280	T19279	arg1Of	activation,cell
R14936	T19282	T19281	arg2Of	induced,was
R14937	T19282	T19283	arg1Of	induced,with
R14938	T19285	T19283	arg2Of	μg/ml,with
R14939	T19285	T19284	arg1Of	μg/ml,10
R14940	T19285	T19286	arg1Of	μg/ml,of
R14941	T19290	T19289	arg2Of	ab,(
R14942	T19290	T19291	arg1Of	ab,'
R14943	T19292	T19289	arg3Of	),(
R14944	T19294	T19286	arg2Of	IgM,of
R14945	T19294	T19287	arg1Of	IgM,goat
R14946	T19294	T19288	arg1Of	IgM,F
R14947	T19294	T19289	arg1Of	IgM,(
R14948	T19294	T19293	arg1Of	IgM,anti-mouse
R14949	T19294	T19295	arg1Of	IgM,(
R14950	T19297	T19295	arg2Of	ImmunoResearch,(
R14951	T19297	T19296	arg1Of	ImmunoResearch,Jackson
R14952	T19298	T19295	arg3Of	),(
R14953	T19299	T19300	arg1Of	Cultures,were
R14954	T19299	T19301	arg2Of	Cultures,pulsed
R14955	T19301	T19300	arg2Of	pulsed,were
R14956	T19301	T19302	arg1Of	pulsed,for
R14957	T19304	T19302	arg2Of	h,for
R14958	T19304	T19303	arg1Of	h,4
R14959	T19304	T19305	arg1Of	h,with
R14960	T19309	T19305	arg2Of	d,with
R14961	T19309	T19306	arg1Of	d,MTS
R14962	T19309	T19307	arg1Of	d,reagent
R14963	T19309	T19308	arg1Of	d,1
R14964	T19309	T19310	arg1Of	d,post-seeding
R15243	T19653	T19651	arg1Of	sections,Paraffin-embedded
R15244	T19653	T19652	arg1Of	sections,spleen
R15245	T19653	T19654	arg1Of	sections,were
R15246	T19653	T19655	arg2Of	sections,deparaffinized
R15247	T19653	T19663	arg2Of	sections,rehydrated
R15248	T19655	T19656	arg1Of	deparaffinized,in
R15249	T19655	T19662	arg1Of	deparaffinized,and
R15250	T19657	T19656	arg2Of	Histoclear,in
R15251	T19657	T19658	arg1Of	Histoclear,(
R15252	T19660	T19658	arg2Of	Diagnostics,(
R15253	T19660	T19659	arg1Of	Diagnostics,National
R15254	T19661	T19658	arg3Of	),(
R15255	T19662	T19654	arg2Of	and,were
R15256	T19663	T19662	arg2Of	rehydrated,and
R15257	T19663	T19664	arg1Of	rehydrated,in
R15258	T19666	T19664	arg2Of	ethanol,in
R15259	T19666	T19665	arg2Of	ethanol,graded
R15260	T19667	T19668	arg1Of	Sections,were
R15261	T19669	T19668	arg2Of	antigen,were
R15262	T19669	T19670	arg2Of	antigen,retrieved
R15263	T19670	T19671	arg1Of	retrieved,by
R15264	T19672	T19671	arg2Of	microwaving,by
R15265	T19672	T19673	arg1Of	microwaving,in
R15266	T19672	T19680	arg1Of	microwaving,for
R15267	T19675	T19673	arg2Of	buffer,in
R15268	T19675	T19674	arg1Of	buffer,citrate
R15269	T19675	T19676	arg1Of	buffer,(
R15270	T19677	T19676	arg2Of	pH,(
R15271	T19677	T19678	arg1Of	pH,6.0
R15272	T19679	T19676	arg3Of	),(
R15273	T19682	T19681	arg1Of	min,15
R15274	T19682	T19683	arg1Of	min,(
R15275	T19682	T19687	arg1Of	min,and
R15276	T19685	T19683	arg2Of	staining,(
R15277	T19685	T19684	arg1Of	staining,LMP1
R15278	T19686	T19683	arg3Of	),(
R15279	T19687	T19680	arg2Of	and,for
R15280	T19689	T19687	arg2Of	min,and
R15281	T19689	T19688	arg1Of	min,10
R15282	T19689	T19690	arg1Of	min,(
R15283	T19692	T19690	arg2Of	staining,(
R15284	T19692	T19691	arg1Of	staining,pStat3
R15285	T19693	T19690	arg3Of	),(
R15286	T19696	T19694	arg2Of	staining,For
R15287	T19696	T19695	arg1Of	staining,LMP1
R15288	T19698	T19699	arg1Of	sections,were
R15289	T19698	T19700	arg2Of	sections,blocked
R15290	T19700	T19694	arg1Of	blocked,For
R15291	T19700	T19697	arg1Of	blocked,","
R15292	T19700	T19699	arg2Of	blocked,were
R15293	T19700	T19701	arg1Of	blocked,with
R15294	T19704	T19702	arg1Of	BSA,1
R15295	T19704	T19703	arg1Of	BSA,%
R15296	T19704	T19705	arg1Of	BSA,and
R15297	T19705	T19701	arg2Of	and,with
R15298	T19705	T19712	arg2Of	and,followed
R15299	T19706	T19707	arg1Of	0.1,%
R15300	T19711	T19705	arg2Of	gelatin,and
R15301	T19711	T19706	arg1Of	gelatin,0.1
R15302	T19711	T19708	arg1Of	gelatin,cold
R15303	T19711	T19709	arg1Of	gelatin,fish
R15304	T19711	T19710	arg1Of	gelatin,skin
R15305	T19712	T19713	arg1Of	followed,with
R15306	T19715	T19713	arg2Of	block,with
R15307	T19715	T19714	arg1Of	block,streptavidin/biotin
R15308	T19715	T19716	arg1Of	block,(
R15309	T19718	T19716	arg2Of	Labs,(
R15310	T19718	T19717	arg1Of	Labs,Vector
R15311	T19719	T19716	arg3Of	),(
R15312	T19722	T19720	arg1Of	anti-LMP1,Rat
R15313	T19722	T19721	arg1Of	anti-LMP1,IgG
R15314	T19722	T19723	arg1Of	anti-LMP1,(
R15315	T19722	T19731	arg1Of	anti-LMP1,were
R15316	T19722	T19732	arg2Of	anti-LMP1,used
R15317	T19725	T19726	arg1Of	8G3,and
R15318	T19726	T19723	arg2Of	and,(
R15319	T19726	T19724	arg1Of	and,clones
R15320	T19726	T19728	arg1Of	and,","
R15321	T19727	T19726	arg2Of	1G6,and
R15322	T19729	T19728	arg2Of	Ascenion,","
R15323	T19730	T19723	arg3Of	),(
R15324	T19732	T19731	arg2Of	used,were
R15325	T19732	T19733	arg1Of	used,at
R15326	T19732	T19738	arg1Of	used,from
R15327	T19732	T19742	arg1Of	used,","
R15328	T19732	T19743	modOf	used,followed
R15329	T19736	T19735	arg1Of	10,:
R15330	T19737	T19733	arg2Of	dilution,at
R15331	T19737	T19734	arg1Of	dilution,1
R15332	T19737	T19736	arg1Of	dilution,10
R15333	T19741	T19738	arg2Of	supernatants,from
R15334	T19741	T19739	arg1Of	supernatants,tissue
R15335	T19741	T19740	arg1Of	supernatants,culture
R15336	T19743	T19744	arg1Of	followed,with
R15337	T19743	T19755	arg1Of	followed,IgG
R15338	T19747	T19746	arg1Of	biotinylated,μg/ml
R15339	T19749	T19744	arg2Of	F,with
R15340	T19749	T19745	arg1Of	F,8
R15341	T19749	T19747	arg1Of	F,biotinylated
R15342	T19749	T19748	arg1Of	F,mouse
R15343	T19751	T19750	arg2Of	ab,(
R15344	T19751	T19752	arg1Of	ab,'
R15345	T19753	T19750	arg3Of	),(
R15346	T19755	T19750	arg1Of	IgG,(
R15347	T19755	T19754	arg1Of	IgG,anti-rat
R15348	T19755	T19759	arg1Of	IgG,pre-adsorbed
R15349	T19757	T19756	arg2Of	H+L,(
R15350	T19758	T19756	arg3Of	),(
R15351	T19759	T19756	arg1Of	pre-adsorbed,(
R15352	T19759	T19760	arg1Of	pre-adsorbed,to
R15353	T19762	T19761	arg1Of	serum,mouse
R15354	T19762	T19763	arg1Of	serum,(
R15355	T19762	T19767	arg1Of	serum,and
R15356	T19765	T19763	arg2Of	ImmunoResearch,(
R15357	T19765	T19764	arg1Of	ImmunoResearch,Jackson
R15358	T19766	T19763	arg3Of	),(
R15359	T19767	T19760	arg2Of	and,to
R15360	T19772	T19767	arg2Of	conjugate,and
R15361	T19772	T19768	arg1Of	conjugate,2
R15362	T19772	T19769	arg1Of	conjugate,μg/ml
R15363	T19772	T19770	arg1Of	conjugate,streptavidin-alkaline
R15364	T19772	T19771	arg1Of	conjugate,phosphatase
R15365	T19772	T19773	arg1Of	conjugate,(
R15366	T19775	T19773	arg2Of	ImmunoResearch,(
R15367	T19775	T19774	arg1Of	ImmunoResearch,Jackson
R15368	T19776	T19773	arg3Of	),(
R15369	T19777	T19778	arg1Of	Stains,were
R15370	T19777	T19779	arg2Of	Stains,developed
R15371	T19777	T19783	arg2Of	Stains,counterstained
R15372	T19779	T19780	arg1Of	developed,with
R15373	T19779	T19782	arg1Of	developed,and
R15374	T19781	T19780	arg2Of	BCIP/NBT,with
R15375	T19782	T19778	arg2Of	and,were
R15376	T19783	T19782	arg2Of	counterstained,and
R15377	T19783	T19784	arg1Of	counterstained,in
R15378	T19787	T19784	arg2Of	Red,in
R15379	T19787	T19785	arg1Of	Red,Nuclear
R15380	T19787	T19786	arg1Of	Red,Fast
R15381	T19787	T19788	arg1Of	Red,(
R15382	T19789	T19788	arg2Of	Dako,(
R15383	T19790	T19788	arg3Of	),(
R15384	T19791	T19792	arg1Of	Phospho-Stat3,was
R15385	T19791	T19793	arg2Of	Phospho-Stat3,detected
R15386	T19791	T19807	arg2Of	Phospho-Stat3,detected
R15387	T19793	T19794	arg1Of	detected,with
R15388	T19793	T19806	arg1Of	detected,and
R15389	T19796	T19794	arg2Of	μg/ml,with
R15390	T19796	T19795	arg1Of	μg/ml,4
R15391	T19796	T19797	arg1Of	μg/ml,of
R15392	T19796	T19800	arg1Of	μg/ml,(
R15393	T19799	T19797	arg2Of	antibody,of
R15394	T19799	T19798	arg1Of	antibody,pStat3
R15395	T19801	T19800	arg2Of	Tyr705,(
R15396	T19801	T19802	arg1Of	Tyr705,","
R15397	T19804	T19802	arg2Of	Signaling,","
R15398	T19804	T19803	arg1Of	Signaling,Cell
R15399	T19805	T19800	arg3Of	),(
R15400	T19806	T19792	arg2Of	and,was
R15401	T19806	T19814	arg1Of	and,(
R15402	T19807	T19806	arg2Of	detected,and
R15403	T19807	T19808	arg1Of	detected,with
R15404	T19813	T19808	arg2Of	kit,with
R15405	T19813	T19809	arg1Of	kit,anti-rabbit
R15406	T19813	T19810	arg1Of	kit,Poly-HRP
R15407	T19813	T19811	arg1Of	kit,IHC
R15408	T19813	T19812	arg1Of	kit,detection
R15409	T19815	T19814	arg2Of	Chemicon,(
R15410	T19816	T19814	arg3Of	),(
R15589	T20046	T20045	arg1Of	cytometry,Flow
R15590	T20048	T20047	arg1Of	million,One
R15591	T20049	T20048	arg1Of	splenocytes,million
R15592	T20049	T20050	arg1Of	splenocytes,were
R15593	T20049	T20051	arg2Of	splenocytes,stained
R15594	T20049	T20059	arg2Of	splenocytes,conjugated
R15595	T20051	T20052	arg1Of	stained,with
R15596	T20051	T20058	arg1Of	stained,or
R15597	T20056	T20052	arg2Of	antibody,with
R15598	T20056	T20053	arg1Of	antibody,the
R15599	T20056	T20054	arg1Of	antibody,appropriate
R15600	T20056	T20055	arg1Of	antibody,primary
R15601	T20056	T20057	arg1Of	antibody,unconjugated
R15602	T20058	T20050	arg2Of	or,were
R15603	T20059	T20058	arg2Of	conjugated,or
R15604	T20059	T20060	arg1Of	conjugated,to
R15605	T20061	T20062	arg1Of	FITC,","
R15606	T20062	T20065	arg1Of	",",or
R15607	T20063	T20062	arg2Of	PE,","
R15608	T20065	T20060	arg2Of	or,to
R15609	T20065	T20064	arg1Of	or,","
R15610	T20065	T20067	arg2Of	or,diluted
R15611	T20066	T20065	arg2Of	APC,or
R15612	T20067	T20068	arg1Of	diluted,in
R15613	T20070	T20068	arg2Of	buffer,in
R15614	T20070	T20069	arg1Of	buffer,stain
R15615	T20070	T20071	arg1Of	buffer,(
R15616	T20072	T20071	arg2Of	PBS,(
R15617	T20072	T20073	arg1Of	PBS,with
R15618	T20074	T20075	arg1Of	3,%
R15619	T20076	T20073	arg2Of	FBS,with
R15620	T20076	T20074	arg1Of	FBS,3
R15621	T20077	T20071	arg3Of	),(
R15622	T20080	T20078	arg2Of	detection,For
R15623	T20080	T20079	arg1Of	detection,BrdU
R15624	T20085	T20082	arg1Of	kit,the
R15625	T20085	T20083	arg1Of	kit,FITC-BrdU
R15626	T20085	T20084	arg1Of	kit,flow
R15627	T20085	T20086	arg1Of	kit,was
R15628	T20085	T20087	arg2Of	kit,used
R15629	T20087	T20078	arg1Of	used,For
R15630	T20087	T20081	arg1Of	used,","
R15631	T20087	T20086	arg2Of	used,was
R15632	T20087	T20088	arg1Of	used,as
R15633	T20089	T20088	arg2Of	instructed,as
R15634	T20092	T20089	arg1Of	manufacturer,instructed
R15635	T20092	T20090	arg2Of	manufacturer,by
R15636	T20092	T20091	arg1Of	manufacturer,the
R15637	T20092	T20093	arg1Of	manufacturer,(
R15638	T20095	T20093	arg2Of	Bioscience,(
R15639	T20095	T20094	arg1Of	Bioscience,BD
R15640	T20096	T20093	arg3Of	),(
R15641	T20099	T20100	arg1Of	cells,were
R15642	T20099	T20101	arg2Of	cells,exposed
R15643	T20099	T20124	arg2Of	cells,permeabilized
R15644	T20101	T20102	arg1Of	exposed,to
R15645	T20101	T20123	arg1Of	exposed,and
R15646	T20103	T20102	arg2Of	EMA,to
R15647	T20103	T20104	arg1Of	EMA,(
R15648	T20103	T20108	arg1Of	EMA,for
R15649	T20106	T20104	arg2Of	Probes,(
R15650	T20106	T20105	arg1Of	Probes,Molecular
R15651	T20107	T20104	arg3Of	),(
R15652	T20109	T20108	arg2Of	exclusion,for
R15653	T20109	T20110	arg1Of	exclusion,of
R15654	T20109	T20113	arg1Of	exclusion,","
R15655	T20109	T20114	arg2Of	exclusion,stained
R15656	T20109	T20118	arg1Of	exclusion,","
R15657	T20109	T20119	arg2Of	exclusion,fixed
R15658	T20112	T20110	arg2Of	cells,of
R15659	T20112	T20111	arg1Of	cells,dead
R15660	T20114	T20115	arg1Of	stained,for
R15661	T20117	T20115	arg2Of	antigens,for
R15662	T20117	T20116	arg1Of	antigens,surface
R15663	T20119	T20120	arg1Of	fixed,in
R15664	T20121	T20120	arg2Of	paraformaldehyde,in
R15665	T20123	T20097	arg1Of	and,Briefly
R15666	T20123	T20098	arg1Of	and,","
R15667	T20123	T20100	arg2Of	and,were
R15668	T20123	T20122	arg1Of	and,","
R15669	T20124	T20123	arg2Of	permeabilized,and
R15670	T20124	T20125	arg1Of	permeabilized,with
R15671	T20126	T20125	arg2Of	saponin,with
R15672	T20128	T20127	arg1Of	expose,To
R15673	T20130	T20128	arg2Of	epitopes,expose
R15674	T20130	T20129	arg1Of	epitopes,BrdU
R15675	T20132	T20128	arg1Of	cells,expose
R15676	T20132	T20133	arg1Of	cells,were
R15677	T20132	T20134	arg2Of	cells,treated
R15678	T20132	T20138	arg2Of	cells,stained
R15679	T20134	T20135	arg1Of	treated,with
R15680	T20134	T20137	arg1Of	treated,and
R15681	T20136	T20135	arg2Of	Dnase,with
R15682	T20137	T20127	modOf	and,To
R15683	T20137	T20131	arg1Of	and,","
R15684	T20137	T20133	arg2Of	and,were
R15685	T20138	T20137	arg2Of	stained,and
R15686	T20138	T20139	arg1Of	stained,with
R15687	T20142	T20139	arg2Of	antibody,with
R15688	T20142	T20140	arg1Of	antibody,FITC-conjugated
R15689	T20142	T20141	arg1Of	antibody,anti-BrdU
R15690	T20144	T20143	arg1Of	cytometry,Flow
R15691	T20144	T20145	arg1Of	cytometry,was
R15692	T20144	T20146	arg2Of	cytometry,performed
R15693	T20146	T20145	arg2Of	performed,was
R15694	T20146	T20147	arg1Of	performed,on
R15695	T20148	T20147	arg2Of	FACScalibur,on
R15696	T20149	T20146	arg3Of	using,performed
R15697	T20152	T20149	arg2Of	program,using
R15698	T20152	T20150	arg1Of	program,the
R15699	T20152	T20151	arg1Of	program,CellQuest
R15700	T20152	T20153	arg1Of	program,(
R15701	T20155	T20153	arg2Of	Dickinson,(
R15702	T20155	T20154	arg1Of	Dickinson,Becton
R15703	T20156	T20153	arg3Of	),(
R15704	T20158	T20157	arg1Of	analysis,Further
R15705	T20158	T20159	arg1Of	analysis,was
R15706	T20158	T20160	arg2Of	analysis,conducted
R15707	T20160	T20159	arg2Of	conducted,was
R15708	T20160	T20161	arg1Of	conducted,on
R15709	T20165	T20161	arg2Of	program,on
R15710	T20165	T20162	arg1Of	program,the
R15711	T20165	T20163	arg1Of	program,Summit
R15712	T20165	T20164	arg1Of	program,v4.2
R15713	T20165	T20166	arg1Of	program,(
R15714	T20167	T20166	arg2Of	Dako,(
R15715	T20168	T20166	arg3Of	),(
R15849	T20346	T20344	arg1Of	assay,Rnase
R15850	T20346	T20345	arg1Of	assay,protection
R15851	T20348	T20347	arg1Of	RNA,Total
R15852	T20348	T20349	arg1Of	RNA,was
R15853	T20348	T20350	arg2Of	RNA,isolated
R15854	T20350	T20349	arg2Of	isolated,was
R15855	T20350	T20351	arg1Of	isolated,from
R15856	T20350	T20365	arg1Of	isolated,","
R15857	T20350	T20366	arg1Of	isolated,according
R15858	T20355	T20351	arg2Of	cells,from
R15859	T20355	T20352	arg1Of	cells,CD19+
R15860	T20355	T20353	arg1Of	cells,MACS-purified
R15861	T20355	T20354	arg1Of	cells,B
R15862	T20355	T20356	arg1Of	cells,using
R15863	T20361	T20356	arg2Of	kit,using
R15864	T20361	T20357	arg1Of	kit,the
R15865	T20361	T20358	arg1Of	kit,Rneasy
R15866	T20361	T20359	arg1Of	kit,midi
R15867	T20361	T20360	arg1Of	kit,purification
R15868	T20361	T20362	arg1Of	kit,with
R15869	T20364	T20362	arg2Of	treatment,with
R15870	T20364	T20363	arg1Of	treatment,Dnase
R15871	T20367	T20366	arg2Of	to,according
R15872	T20369	T20368	arg1Of	manufacturer,the
R15873	T20369	T20370	arg2Of	manufacturer,'s
R15874	T20371	T20367	arg2Of	instructions,to
R15875	T20371	T20370	arg1Of	instructions,'s
R15876	T20371	T20372	arg1Of	instructions,(
R15877	T20373	T20372	arg2Of	Qiagen,(
R15878	T20374	T20372	arg3Of	),(
R15879	T20379	T20375	arg1Of	set,The
R15880	T20379	T20376	arg1Of	set,mCK-1
R15881	T20379	T20377	arg1Of	set,probe
R15882	T20379	T20378	arg1Of	set,template
R15883	T20379	T20380	arg1Of	set,(
R15884	T20379	T20384	arg1Of	set,was
R15885	T20379	T20385	arg2Of	set,labeled
R15886	T20382	T20380	arg2Of	Biosciences,(
R15887	T20382	T20381	arg1Of	Biosciences,BD
R15888	T20383	T20380	arg3Of	),(
R15889	T20385	T20384	arg2Of	labeled,was
R15890	T20385	T20386	modOf	labeled,using
R15891	T20386	T20392	arg1Of	using,according
R15892	T20389	T20388	arg1Of	Vitro,In
R15893	T20391	T20386	arg2Of	Kit,using
R15894	T20391	T20387	arg1Of	Kit,the
R15895	T20391	T20389	arg1Of	Kit,Vitro
R15896	T20391	T20390	arg1Of	Kit,Transcription
R15897	T20393	T20392	arg2Of	to,according
R15898	T20395	T20394	arg1Of	manufacturer,the
R15899	T20395	T20396	arg2Of	manufacturer,'s
R15900	T20397	T20393	arg2Of	instructions,to
R15901	T20397	T20396	arg1Of	instructions,'s
R15902	T20397	T20398	arg1Of	instructions,(
R15903	T20400	T20398	arg2Of	Biosciences,(
R15904	T20400	T20399	arg1Of	Biosciences,BD
R15905	T20401	T20398	arg3Of	),(
R15906	T20405	T20404	arg1Of	ng,50
R15907	T20405	T20406	arg1Of	ng,of
R15908	T20405	T20410	arg1Of	ng,was
R15909	T20405	T20411	arg2Of	ng,labeled
R15910	T20409	T20406	arg2Of	set,of
R15911	T20409	T20407	arg1Of	set,mCK-1
R15912	T20409	T20408	arg1Of	set,probe
R15913	T20411	T20402	arg1Of	labeled,Briefly
R15914	T20411	T20403	arg1Of	labeled,","
R15915	T20411	T20410	arg2Of	labeled,was
R15916	T20411	T20412	arg1Of	labeled,with
R15917	T20414	T20413	arg2Of	α-32P,[
R15918	T20415	T20413	arg3Of	],[
R15919	T20416	T20412	arg2Of	UTP,with
R15920	T20416	T20413	arg1Of	UTP,[
R15921	T20416	T20417	arg1Of	UTP,using
R15922	T20416	T20422	arg2Of	UTP,purified
R15923	T20417	T20421	arg1Of	using,and
R15924	T20420	T20417	arg2Of	polymerase,using
R15925	T20420	T20418	arg1Of	polymerase,T7
R15926	T20420	T20419	arg1Of	polymerase,RNA
R15927	T20422	T20421	arg2Of	purified,and
R15928	T20422	T20423	modOf	purified,using
R15929	T20426	T20423	arg2Of	columns,using
R15930	T20426	T20424	arg1Of	columns,Sephadex
R15931	T20426	T20425	arg1Of	columns,G-50
R15932	T20426	T20427	arg1Of	columns,(
R15933	T20429	T20428	arg1Of	Spin,NucAway
R15934	T20430	T20427	arg2Of	column,(
R15935	T20430	T20429	arg1Of	column,Spin
R15936	T20430	T20431	arg1Of	column,","
R15937	T20432	T20431	arg2Of	Ambion,","
R15938	T20433	T20427	arg3Of	),(
R15939	T20436	T20434	arg1Of	probe,The
R15940	T20436	T20435	arg2Of	probe,labeled
R15941	T20436	T20437	arg1Of	probe,was
R15942	T20436	T20438	arg2Of	probe,quantitated
R15943	T20436	T20439	arg1Of	probe,using
R15944	T20438	T20437	arg2Of	quantitated,was
R15945	T20438	T20444	arg1Of	quantitated,and
R15946	T20439	T20438	arg3Of	using,quantitated
R15947	T20442	T20439	arg2Of	counter,using
R15948	T20442	T20440	arg1Of	counter,a
R15949	T20442	T20441	arg1Of	counter,scintillation
R15950	T20444	T20443	arg1Of	and,","
R15951	T20447	T20446	arg1Of	105,×
R15952	T20449	T20445	arg1Of	cpm,6
R15953	T20449	T20447	arg1Of	cpm,105
R15954	T20449	T20448	arg1Of	cpm,cherenkov
R15955	T20449	T20450	arg1Of	cpm,was
R15956	T20449	T20451	arg2Of	cpm,used
R15957	T20449	T20453	arg1Of	cpm,hybridize
R15958	T20451	T20444	arg2Of	used,and
R15959	T20451	T20450	arg2Of	used,was
R15960	T20453	T20451	arg3Of	hybridize,used
R15961	T20453	T20452	arg1Of	hybridize,to
R15962	T20453	T20454	arg1Of	hybridize,to
R15963	T20456	T20454	arg2Of	μg,to
R15964	T20456	T20455	arg1Of	μg,4
R15965	T20456	T20457	arg1Of	μg,of
R15966	T20459	T20457	arg2Of	RNA,of
R15967	T20459	T20458	arg1Of	RNA,total
R15968	T20459	T20460	arg1Of	RNA,using
R15969	T20463	T20460	arg2Of	kit,using
R15970	T20463	T20461	arg1Of	kit,the
R15971	T20463	T20462	arg1Of	kit,RPA
R15972	T20463	T20464	arg1Of	kit,(
R15973	T20466	T20464	arg2Of	Biosciences,(
R15974	T20466	T20465	arg1Of	Biosciences,BD
R15975	T20467	T20464	arg3Of	),(
R15976	T20468	T20469	arg1Of	Samples,were
R15977	T20468	T20470	arg2Of	Samples,denatured
R15978	T20468	T20475	arg1Of	Samples,hybridized
R15979	T20470	T20469	arg2Of	denatured,were
R15980	T20470	T20471	arg1Of	denatured,at
R15981	T20470	T20474	arg1Of	denatured,and
R15982	T20473	T20471	arg2Of	°C,at
R15983	T20473	T20472	arg1Of	°C,90
R15984	T20475	T20474	arg2Of	hybridized,and
R15985	T20475	T20476	arg1Of	hybridized,at
R15986	T20478	T20476	arg2Of	°C,at
R15987	T20478	T20477	arg1Of	°C,56
R15988	T20478	T20479	arg1Of	°C,overnight
R15989	T20481	T20480	arg1Of	RNA,Single-stranded
R15990	T20481	T20482	arg1Of	RNA,was
R15991	T20481	T20483	arg2Of	RNA,digested
R15992	T20481	T20494	arg2Of	RNA,precipitated
R15993	T20481	T20495	arg1Of	RNA,using
R15994	T20483	T20484	arg1Of	digested,with
R15995	T20483	T20493	arg1Of	digested,and
R15996	T20486	T20484	arg2Of	mixture,with
R15997	T20486	T20485	arg1Of	mixture,a
R15998	T20486	T20487	arg1Of	mixture,of
R15999	T20489	T20488	arg1Of	A,Rnase
R16000	T20489	T20490	arg1Of	A,and
R16001	T20490	T20487	arg2Of	and,of
R16002	T20491	T20490	arg2Of	T1,and
R16003	T20493	T20482	arg2Of	and,was
R16004	T20493	T20492	arg1Of	and,","
R16005	T20494	T20493	arg2Of	precipitated,and
R16006	T20495	T20494	arg3Of	using,precipitated
R16007	T20496	T20495	arg2Of	isopropanol,using
R16008	T20498	T20497	arg2Of	probes,Protected
R16009	T20498	T20499	arg1Of	probes,were
R16010	T20498	T20500	arg2Of	probes,resolved
R16011	T20498	T20512	arg2Of	probes,imaged
R16012	T20498	T20513	arg1Of	probes,using
R16013	T20500	T20501	arg1Of	resolved,on
R16014	T20500	T20511	arg1Of	resolved,and
R16015	T20504	T20505	arg1Of	4.75,%
R16016	T20507	T20501	arg2Of	gel,on
R16017	T20507	T20502	arg1Of	gel,a
R16018	T20507	T20503	arg1Of	gel,denaturing
R16019	T20507	T20504	arg1Of	gel,4.75
R16020	T20507	T20506	arg1Of	gel,acrylamide
R16021	T20507	T20508	arg1Of	gel,","
R16022	T20507	T20509	arg2Of	gel,dried
R16023	T20511	T20499	arg2Of	and,were
R16024	T20511	T20510	arg1Of	and,","
R16025	T20512	T20511	arg2Of	imaged,and
R16026	T20513	T20512	arg3Of	using,imaged
R16027	T20515	T20513	arg2Of	phosphorImager,using
R16028	T20515	T20514	arg1Of	phosphorImager,a
R16029	T20515	T20516	arg1Of	phosphorImager,(
R16030	T20518	T20516	arg2Of	Dynamics,(
R16031	T20518	T20517	arg1Of	Dynamics,Molecular
R16032	T20519	T20516	arg3Of	),(
R16033	T20520	T20521	arg1Of	Densitometry,was
R16034	T20520	T20522	arg2Of	Densitometry,performed
R16035	T20522	T20521	arg2Of	performed,was
R16036	T20523	T20522	arg3Of	using,performed
R16037	T20528	T20523	arg2Of	software,using
R16038	T20528	T20524	arg1Of	software,the
R16039	T20528	T20525	arg1Of	software,ImageQuant
R16040	T20528	T20526	arg1Of	software,TL
R16041	T20528	T20527	arg1Of	software,v2005
R16042	T20528	T20529	arg1Of	software,(
R16043	T20531	T20529	arg2Of	Healthcare,(
R16044	T20531	T20530	arg1Of	Healthcare,GE
R16045	T20532	T20529	arg3Of	),(
R16247	T20774	T20773	arg1Of	analysis,PCR
R16248	T20774	T20775	arg1Of	analysis,of
R16249	T20778	T20775	arg2Of	rearrangement,of
R16250	T20778	T20776	arg1Of	rearrangement,κ
R16251	T20778	T20777	arg1Of	rearrangement,chain
R16252	T20779	T20780	arg1Of	DNA,was
R16253	T20779	T20781	arg2Of	DNA,isolated
R16254	T20781	T20780	arg2Of	isolated,was
R16255	T20781	T20782	arg1Of	isolated,from
R16256	T20781	T20792	arg1Of	isolated,","
R16257	T20781	T20793	arg1Of	isolated,with
R16258	T20783	T20782	arg2Of	splenocytes,from
R16259	T20783	T20784	arg1Of	splenocytes,using
R16260	T20788	T20784	arg2Of	Kit,using
R16261	T20788	T20785	arg1Of	Kit,the
R16262	T20788	T20786	arg1Of	Kit,Dneasy
R16263	T20788	T20787	arg1Of	Kit,Tissue
R16264	T20788	T20789	arg1Of	Kit,(
R16265	T20790	T20789	arg2Of	Qiagen,(
R16266	T20791	T20789	arg3Of	),(
R16267	T20795	T20793	arg2Of	treatment,with
R16268	T20795	T20794	arg1Of	treatment,Rnase
R16269	T20797	T20796	arg1Of	reactions,PCR
R16270	T20797	T20798	arg1Of	reactions,contained
R16271	T20800	T20798	arg2Of	ng,contained
R16272	T20800	T20799	arg1Of	ng,100
R16273	T20800	T20801	arg1Of	ng,of
R16274	T20803	T20802	arg1Of	DNA,genomic
R16275	T20803	T20804	arg1Of	DNA,","
R16276	T20804	T20809	arg1Of	",",","
R16277	T20806	T20804	arg2Of	μM,","
R16278	T20806	T20805	arg1Of	μM,0.2
R16279	T20806	T20808	arg1Of	μM,primer
R16280	T20808	T20807	arg1Of	primer,each
R16281	T20809	T20814	arg1Of	",",and
R16282	T20812	T20809	arg2Of	dNTPs,","
R16283	T20812	T20810	arg1Of	dNTPs,0.2
R16284	T20812	T20811	arg1Of	dNTPs,mM
R16285	T20814	T20801	arg2Of	and,of
R16286	T20814	T20813	arg1Of	and,","
R16287	T20814	T20823	arg2Of	and,performed
R16288	T20815	T20816	arg1Of	2.5,U
R16289	T20819	T20814	arg2Of	polymerase,and
R16290	T20819	T20815	arg1Of	polymerase,2.5
R16291	T20819	T20817	arg1Of	polymerase,Taq
R16292	T20819	T20818	arg1Of	polymerase,DNA
R16293	T20819	T20820	arg1Of	polymerase,(
R16294	T20821	T20820	arg2Of	NEB,(
R16295	T20822	T20820	arg3Of	),(
R16296	T20823	T20824	arg1Of	performed,in
R16297	T20827	T20824	arg2Of	buffer,in
R16298	T20827	T20825	arg1Of	buffer,1X
R16299	T20827	T20826	arg1Of	buffer,ThermoPol
R16300	T20827	T20828	arg1Of	buffer,(
R16301	T20829	T20828	arg2Of	NEB,(
R16302	T20830	T20828	arg3Of	),(
R16303	T20831	T20832	arg2Of	Primers,used
R16304	T20831	T20833	arg1Of	Primers,were
R16305	T20831	T20838	arg1Of	Primers,have
R16306	T20831	T20839	arg1Of	Primers,been
R16307	T20831	T20840	arg2Of	Primers,described
R16308	T20833	T20837	arg1Of	were,and
R16309	T20834	T20835	arg1Of	Vκcon,and
R16310	T20835	T20833	arg2Of	and,were
R16311	T20836	T20835	arg2Of	Jκ5–1°,and
R16312	T20840	T20837	arg2Of	described,and
R16313	T20840	T20838	arg2Of	described,have
R16314	T20840	T20839	arg2Of	described,been
R16315	T20840	T20841	arg1Of	described,previously
R16316	T20840	T20842	arg1Of	described,[
R16317	T20843	T20842	arg2Of	74,[
R16318	T20844	T20842	arg3Of	],[
R16319	T20846	T20845	arg1Of	conditions,PCR
R16320	T20846	T20847	arg1Of	conditions,were
R16321	T20847	T20850	arg1Of	were,for
R16324	T20849	T20847	arg2Of	°C,were
R16325	T20849	T20848	arg1Of	°C,94
R16326	T20852	T20851	arg1Of	min,2
R16327	T20852	T20853	arg1Of	min,","
R16329	T20855	T20853	arg2Of	cycles,","
R16330	T20855	T20854	arg1Of	cycles,40
R16331	T20855	T20856	arg1Of	cycles,of
R16332	T20858	T20856	arg2Of	°C,of
R16333	T20858	T20857	arg1Of	°C,94
R16343	T20869	T20850	arg2Of	and,for
R16484	T21035	T21034	arg1Of	analysis,Immunoblot
R16485	T21038	T21036	arg1Of	lysates,Whole
R16486	T21038	T21037	arg1Of	lysates,cell
R16487	T21038	T21039	arg1Of	lysates,were
R16488	T21038	T21040	arg2Of	lysates,prepared
R16489	T21040	T21039	arg2Of	prepared,were
R16490	T21040	T21041	arg1Of	prepared,in
R16491	T21040	T21047	arg1Of	prepared,buffer
R16494	T21043	T21041	arg2Of	assay,in
R16495	T21045	T21044	arg2Of	RIPA,(
R16496	T21046	T21044	arg3Of	),(
R16497	T21047	T21048	arg1Of	buffer,(
R16498	T21047	T21078	arg2Of	buffer,supplemented
R16499	T21049	T21050	arg1Of	20,mM
R16500	T21051	T21049	arg1Of	Tris-HCl,20
R16501	T21051	T21052	arg1Of	Tris-HCl,[
R16502	T21051	T21056	arg1Of	Tris-HCl,","
R16503	T21053	T21052	arg2Of	pH,[
R16504	T21053	T21054	arg1Of	pH,7.5
R16505	T21055	T21052	arg3Of	],[
R16506	T21056	T21048	arg2Of	",",(
R16507	T21057	T21058	arg1Of	150,mM
R16508	T21059	T21057	arg1Of	NaCl,150
R16509	T21059	T21060	arg1Of	NaCl,","
R16510	T21060	T21064	arg1Of	",",","
R16511	T21061	T21062	arg1Of	1,mM
R16512	T21063	T21060	arg2Of	EDTA,","
R16513	T21063	T21061	arg1Of	EDTA,1
R16514	T21064	T21068	arg1Of	",",","
R16515	T21065	T21066	arg1Of	1,%
R16516	T21067	T21064	arg2Of	NP-40,","
R16517	T21067	T21065	arg1Of	NP-40,1
R16518	T21068	T21072	arg1Of	",",","
R16519	T21069	T21070	arg1Of	0.1,%
R16520	T21071	T21068	arg2Of	SDS,","
R16521	T21071	T21069	arg1Of	SDS,0.1
R16522	T21072	T21056	arg2Of	",",","
R16523	T21073	T21074	arg1Of	0.1,%
R16524	T21076	T21072	arg2Of	deoxycholate,","
R16525	T21076	T21073	arg1Of	deoxycholate,0.1
R16526	T21076	T21075	arg1Of	deoxycholate,sodium
R16527	T21077	T21048	arg3Of	),(
R16528	T21078	T21079	arg1Of	supplemented,with
R16529	T21080	T21081	arg1Of	2,mM
R16530	T21083	T21080	arg1Of	fluoride,2
R16531	T21083	T21082	arg1Of	fluoride,phenylmethylsulfonyl
R16532	T21083	T21084	arg1Of	fluoride,","
R16533	T21084	T21089	arg1Of	",",and
R16534	T21085	T21086	arg1Of	1,mM
R16535	T21087	T21084	arg2Of	Na3VO4,","
R16536	T21087	T21085	arg1Of	Na3VO4,1
R16537	T21089	T21079	arg2Of	and,with
R16538	T21089	T21088	arg1Of	and,","
R16539	T21092	T21090	arg1Of	100,1
R16540	T21092	T21091	arg1Of	100,:
R16541	T21095	T21089	arg2Of	cocktails,and
R16542	T21095	T21092	arg1Of	cocktails,100
R16543	T21095	T21093	arg1Of	cocktails,protease/phosphatase
R16544	T21095	T21094	arg1Of	cocktails,inhibitor
R16545	T21095	T21096	arg1Of	cocktails,(
R16546	T21097	T21096	arg2Of	Sigma,(
R16547	T21098	T21096	arg3Of	),(
R16548	T21100	T21099	arg1Of	lysates,Crude
R16549	T21100	T21101	arg1Of	lysates,were
R16550	T21100	T21102	arg2Of	lysates,centrifuged
R16551	T21102	T21101	arg2Of	centrifuged,were
R16552	T21102	T21103	arg1Of	centrifuged,at
R16553	T21102	T21106	arg1Of	centrifuged,for
R16554	T21102	T21109	arg1Of	centrifuged,at
R16555	T21102	T21112	arg1Of	centrifuged,and
R16556	T21105	T21103	arg2Of	rpm,at
R16557	T21105	T21104	arg1Of	rpm,"13,000"
R16558	T21108	T21106	arg2Of	min,for
R16559	T21108	T21107	arg1Of	min,10
R16560	T21111	T21109	arg2Of	°C,at
R16561	T21111	T21110	arg1Of	°C,4
R16562	T21114	T21113	arg1Of	supernatants,the
R16563	T21114	T21115	arg1Of	supernatants,were
R16564	T21114	T21116	arg2Of	supernatants,collected
R16565	T21116	T21112	arg2Of	collected,and
R16566	T21116	T21115	arg2Of	collected,were
R16567	T21116	T21117	arg1Of	collected,for
R16568	T21119	T21117	arg2Of	analysis,for
R16569	T21119	T21118	arg1Of	analysis,further
R16570	T21121	T21120	arg1Of	concentrations,Protein
R16571	T21121	T21122	arg1Of	concentrations,were
R16572	T21121	T21123	arg2Of	concentrations,determined
R16573	T21123	T21122	arg2Of	determined,were
R16574	T21123	T21124	arg1Of	determined,with
R16575	T21130	T21124	arg2Of	system,with
R16576	T21130	T21125	arg1Of	system,the
R16577	T21130	T21126	arg1Of	system,Bio-Rad
R16578	T21130	T21127	arg1Of	system,DC
R16579	T21130	T21128	arg1Of	system,protein
R16580	T21130	T21129	arg1Of	system,assay
R16581	T21131	T21132	arg1Of	Lysates,were
R16582	T21131	T21133	arg2Of	Lysates,boiled
R16583	T21131	T21148	arg2Of	Lysates,transferred
R16584	T21133	T21134	arg1Of	boiled,in
R16585	T21133	T21147	arg1Of	boiled,and
R16586	T21136	T21134	arg2Of	presence,in
R16587	T21136	T21135	arg1Of	presence,the
R16588	T21136	T21137	arg1Of	presence,of
R16589	T21136	T21141	arg1Of	presence,","
R16590	T21136	T21142	arg2Of	presence,separated
R16591	T21138	T21139	arg1Of	2.5,%
R16592	T21140	T21137	arg2Of	β-mecaptoethanol,of
R16593	T21140	T21138	arg1Of	β-mecaptoethanol,2.5
R16594	T21145	T21142	arg1Of	SDS-PAGE,separated
R16595	T21145	T21143	arg2Of	SDS-PAGE,by
R16596	T21145	T21144	arg1Of	SDS-PAGE,denaturing
R16597	T21147	T21132	arg2Of	and,were
R16598	T21147	T21146	arg1Of	and,","
R16599	T21148	T21147	arg2Of	transferred,and
R16600	T21148	T21149	arg1Of	transferred,to
R16601	T21148	T21153	arg1Of	transferred,(
R16602	T21148	T21158	arg1Of	transferred,in
R16603	T21152	T21149	arg2Of	membranes,to
R16604	T21152	T21150	arg1Of	membranes,0.45-μm
R16605	T21152	T21151	arg1Of	membranes,Optitran
R16606	T21156	T21153	arg2Of	Schuell,(
R16607	T21156	T21154	arg1Of	Schuell,Schleicher
R16608	T21156	T21155	arg1Of	Schuell,&
R16609	T21157	T21153	arg3Of	),(
R16610	T21162	T21158	arg2Of	unit,in
R16611	T21162	T21159	arg1Of	unit,a
R16612	T21162	T21160	arg1Of	unit,Bio-Rad
R16613	T21162	T21161	arg1Of	unit,transfer
R16614	T21163	T21164	arg1Of	Membranes,were
R16615	T21163	T21165	arg2Of	Membranes,immunoblotted
R16616	T21165	T21164	arg2Of	immunoblotted,were
R16617	T21165	T21166	arg1Of	immunoblotted,with
R16618	T21170	T21166	arg2Of	antibody,with
R16619	T21170	T21167	arg1Of	antibody,the
R16620	T21170	T21168	arg1Of	antibody,appropriate
R16621	T21170	T21169	arg1Of	antibody,primary
R16622	T21170	T21171	arg2Of	antibody,followed
R16623	T21173	T21171	arg1Of	horseradish,followed
R16624	T21173	T21172	arg2Of	horseradish,by
R16625	T21173	T21174	arg2Of	horseradish,peroxidase–tagged
R16626	T21173	T21184	arg2Of	horseradish,detected
R16627	T21174	T21183	arg1Of	peroxidase–tagged,and
R16628	T21176	T21174	arg3Of	antibodies,peroxidase–tagged
R16629	T21176	T21175	arg1Of	antibodies,secondary
R16630	T21176	T21177	arg1Of	antibodies,(
R16631	T21179	T21178	arg1Of	Biosciences,Amersham
R16632	T21179	T21180	arg1Of	Biosciences,and
R16633	T21180	T21177	arg2Of	and,(
R16634	T21181	T21180	arg2Of	Dako,and
R16635	T21182	T21177	arg3Of	),(
R16636	T21184	T21183	arg2Of	detected,and
R16637	T21184	T21185	arg1Of	detected,with
R16638	T21190	T21185	arg2Of	System,with
R16639	T21190	T21186	arg1Of	System,the
R16640	T21190	T21187	arg1Of	System,SuperSignal
R16641	T21190	T21188	arg1Of	System,West
R16642	T21190	T21189	arg1Of	System,Pico
R16643	T21190	T21191	arg1Of	System,(
R16644	T21192	T21191	arg2Of	Pierce,(
R16645	T21193	T21191	arg3Of	),(
R16492	T21043	T21042	arg1Of	assay,radioimmunoprecipitation
R16493	T21043	T21044	arg1Of	assay,(
R16322	T20847	T20875	arg1Of	were,","
R16323	T20847	T20876	modOf	were,followed
R16328	T20853	T20862	arg1Of	",",","
R16334	T20858	T20859	arg1Of	°C,for
R16335	T20861	T20859	arg2Of	s,for
R16336	T20861	T20860	arg1Of	s,30
R16337	T20862	T20869	arg1Of	",",and
R16338	T20864	T20862	arg2Of	°C,","
R16339	T20864	T20863	arg1Of	°C,63
R16340	T20864	T20865	arg1Of	°C,for
R16341	T20867	T20865	arg2Of	s,for
R16342	T20867	T20866	arg1Of	s,90
R16344	T20869	T20868	arg1Of	and,","
R16345	T20871	T20869	arg2Of	°C,and
R16346	T20871	T20870	arg1Of	°C,72
R16347	T20871	T20872	arg1Of	°C,for
R16348	T20874	T20872	arg2Of	min,for
R16349	T20874	T20873	arg1Of	min,1
R16350	T20879	T20876	arg1Of	cycle,followed
R16351	T20879	T20877	arg2Of	cycle,by
R16352	T20879	T20878	arg1Of	cycle,1
R16353	T20879	T20880	arg1Of	cycle,of
R16354	T20882	T20880	arg2Of	°C,of
R16355	T20882	T20881	arg1Of	°C,72
R16356	T20882	T20883	arg1Of	°C,for
R16357	T20885	T20883	arg2Of	min,for
R16358	T20885	T20884	arg1Of	min,5

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T18431	23-33	NNP	denotes	Transgenic
T18432	34-38	NNS	denotes	mice
T18433	38-39	.	denotes	.
T18434	40-50	NNP	denotes	Generation
T18435	51-53	IN	denotes	of
T18436	54-58	NNP	denotes	LMP1
T18437	59-63	NNS	denotes	mice
T18438	64-69	IN	denotes	under
T18439	70-73	DT	denotes	the
T18440	74-76	NNP	denotes	Ig
T18441	77-82	JJ	denotes	heavy
T18442	83-88	NN	denotes	chain
T18443	89-97	NN	denotes	promoter
T18444	98-101	CC	denotes	and
T18445	102-110	NN	denotes	enhancer
T18446	111-115	VBP	denotes	have
T18447	116-120	VBN	denotes	been
T18448	121-130	VBN	denotes	described
T18449	131-141	RB	denotes	previously
T18450	142-145	CC	denotes	and
T18451	146-150	VBD	denotes	were
T18452	151-161	VBN	denotes	maintained
T18453	162-164	IN	denotes	as
T18454	165-178	NNS	denotes	heterozygotes
T18455	179-181	IN	denotes	on
T18456	182-183	DT	denotes	a
T18457	184-189	NNP	denotes	Balbc
T18458	190-200	NN	denotes	background
T18459	201-202	NNP	denotes	[
T18460	202-204	CD	denotes	26
T18461	204-205	NNP	denotes	]
T18462	205-206	.	denotes	.
T18463	207-211	CD	denotes	LMP1
T18464	212-216	NNS	denotes	mice
T18465	217-221	VBD	denotes	were
T18466	222-231	VBN	denotes	genotyped
T18467	232-234	IN	denotes	by
T18468	235-243	NNP	denotes	Southern
T18469	244-248	NN	denotes	blot
T18470	249-252	CC	denotes	and
T18471	253-256	NNP	denotes	PCR
T18472	257-265	NN	denotes	analysis
T18473	266-268	IN	denotes	of
T18474	269-273	NN	denotes	tail
T18475	274-277	NN	denotes	DNA
T18476	278-280	IN	denotes	as
T18477	281-290	VBN	denotes	described
T18478	291-301	RB	denotes	previously
T18479	302-303	NNP	denotes	[
T18480	303-305	CD	denotes	26
T18481	305-306	NNP	denotes	]
T18482	306-307	.	denotes	.
T18483	308-314	NNP	denotes	Spleen
T18484	315-318	CC	denotes	and
T18485	319-324	JJR	denotes	liver
T18486	325-333	NNS	denotes	sections
T18487	334-338	VBD	denotes	were
T18488	339-344	VBN	denotes	fixed
T18489	345-347	IN	denotes	in
T18490	348-349	CD	denotes	4
T18491	349-350	NN	denotes	%
T18492	351-367	NN	denotes	paraformaldehyde
T18493	368-371	CC	denotes	and
T18494	372-380	VBN	denotes	embedded
T18495	381-383	IN	denotes	in
T18496	384-392	NN	denotes	paraffin
T18497	392-393	,	denotes	,
T18498	394-397	CC	denotes	and
T18499	398-402	JJ	denotes	5-μm
T18500	403-411	NNS	denotes	sections
T18501	412-416	VBD	denotes	were
T18502	417-424	VBN	denotes	stained
T18503	425-429	IN	denotes	with
T18504	430-441	NN	denotes	hematoxylin
T18505	442-445	CC	denotes	and
T18506	446-451	NN	denotes	eosin
T18507	452-455	IN	denotes	for
T18508	456-473	JJ	denotes	histopathological
T18509	474-482	NN	denotes	analysis
T18510	482-483	.	denotes	.
T18511	484-493	NNP	denotes	Lymphomas
T18512	494-498	VBD	denotes	were
T18513	499-507	VBN	denotes	passaged
T18514	508-510	IN	denotes	by
T18515	511-526	JJ	denotes	intraperitoneal
T18516	527-536	NN	denotes	injection
T18517	537-539	IN	denotes	of
T18518	540-541	CD	denotes	1
T18519	542-543	CD	denotes	×
T18520	544-547	CD	denotes	108
T18521	548-559	NNS	denotes	splenocytes
T18522	560-564	IN	denotes	into
T18523	565-569	NNP	denotes	SCID
T18524	570-574	NNS	denotes	mice
T18525	575-578	CC	denotes	and
T18526	579-589	JJ	denotes	sacrificed
T18527	590-594	IN	denotes	upon
T18528	595-606	NN	denotes	development
T18529	607-609	IN	denotes	of
T18530	610-612	DT	denotes	an
T18531	613-621	JJ	denotes	extended
T18532	622-629	NNS	denotes	abdomen
T18533	629-630	.	denotes	.
T18534	631-638	NNS	denotes	Animals
T18535	639-643	VBD	denotes	were
T18536	644-650	VBN	denotes	housed
T18537	651-653	IN	denotes	in
T18538	654-657	DT	denotes	the
T18539	658-669	NNP	denotes	Association
T18540	670-673	IN	denotes	for
T18541	674-684	NNP	denotes	Assessment
T18542	685-688	CC	denotes	and
T18543	689-702	NNP	denotes	Accreditation
T18544	703-706	IN	denotes	for
T18545	707-713	NNP	denotes	Animal
T18546	714-718	NNP	denotes	Care
T18547	719-727	VBD	denotes	approved
T18548	728-734	JJ	denotes	animal
T18549	735-743	NN	denotes	facility
T18550	744-746	IN	denotes	at
T18551	747-750	DT	denotes	the
T18552	751-761	NNP	denotes	University
T18553	762-764	IN	denotes	of
T18554	765-770	NNP	denotes	North
T18555	771-779	NNP	denotes	Carolina
T18556	780-782	IN	denotes	at
T18557	783-789	NNP	denotes	Chapel
T18558	790-794	NNP	denotes	Hill
T18559	794-795	.	denotes	.
T18560	796-799	DT	denotes	All
T18561	800-809	NNS	denotes	protocols
T18562	810-814	VBD	denotes	were
T18563	815-823	VBN	denotes	approved
T18564	824-826	IN	denotes	by
T18565	827-830	DT	denotes	the
T18566	831-844	JJ	denotes	Institutional
T18567	845-851	NNP	denotes	Animal
T18568	852-856	NNP	denotes	Care
T18569	857-860	CC	denotes	and
T18570	861-864	NNP	denotes	Use
T18571	865-874	NNP	denotes	Committee
T18572	874-875	.	denotes	.
T18800	877-886	NN	denotes	Isolation
T18801	887-890	CC	denotes	and
T18802	891-897	NN	denotes	growth
T18803	898-900	IN	denotes	of
T18804	901-902	NNP	denotes	B
T18805	903-908	NNS	denotes	cells
T18806	908-909	.	denotes	.
T18807	910-921	NNS	denotes	Splenocytes
T18808	922-926	VBD	denotes	were
T18809	927-935	VBN	denotes	prepared
T18810	936-938	IN	denotes	by
T18811	939-951	VBG	denotes	homogenizing
T18812	952-958	JJ	denotes	spleen
T18813	959-965	NN	denotes	tissue
T18814	966-970	IN	denotes	with
T18815	971-974	CD	denotes	two
T18816	975-982	JJ	denotes	frosted
T18817	983-989	NNS	denotes	slides
T18818	990-993	CC	denotes	and
T18819	994-1000	NN	denotes	debris
T18820	1001-1004	VBD	denotes	was
T18821	1005-1013	VBN	denotes	filtered
T18822	1014-1021	IN	denotes	through
T18823	1022-1023	DT	denotes	a
T18824	1024-1030	JJ	denotes	100-μm
T18825	1031-1035	NN	denotes	cell
T18826	1036-1044	NN	denotes	strainer
T18827	1044-1045	.	denotes	.
T18828	1046-1058	NNS	denotes	Erythrocytes
T18829	1059-1063	VBD	denotes	were
T18830	1064-1069	VBN	denotes	lysed
T18831	1070-1075	VBG	denotes	using
T18832	1076-1079	CD	denotes	0.8
T18833	1079-1080	NN	denotes	%
T18834	1081-1089	NN	denotes	ammonium
T18835	1090-1098	NN	denotes	chloride
T18836	1099-1107	NN	denotes	solution
T18837	1108-1109	-LRB-	denotes	(
T18838	1109-1117	NNP	denotes	StemCell
T18839	1118-1130	NNPS	denotes	Technologies
T18840	1130-1131	-RRB-	denotes	)
T18841	1132-1135	IN	denotes	for
T18842	1136-1138	CD	denotes	10
T18843	1139-1142	NN	denotes	min
T18844	1143-1145	IN	denotes	on
T18845	1146-1149	NN	denotes	ice
T18846	1150-1153	CC	denotes	and
T18847	1154-1160	VBD	denotes	washed
T18848	1161-1166	RB	denotes	twice
T18849	1167-1171	IN	denotes	with
T18850	1172-1175	NNP	denotes	PBS
T18851	1175-1176	.	denotes	.
T18852	1177-1178	NNP	denotes	B
T18853	1179-1184	NNS	denotes	cells
T18854	1185-1189	VBD	denotes	were
T18855	1190-1198	VBN	denotes	isolated
T18856	1199-1204	VBG	denotes	using
T18857	1205-1214	NN	denotes	CD19-MACS
T18858	1215-1220	NNS	denotes	beads
T18859	1221-1230	VBG	denotes	according
T18860	1231-1233	TO	denotes	to
T18861	1234-1237	DT	denotes	the
T18862	1238-1250	NN	denotes	manufacturer
T18863	1250-1252	POS	denotes	's
T18864	1253-1265	NNS	denotes	instructions
T18865	1266-1267	-LRB-	denotes	(
T18866	1267-1275	NNP	denotes	Miltenyi
T18867	1276-1282	NNP	denotes	Biotec
T18868	1282-1283	-RRB-	denotes	)
T18869	1284-1287	CC	denotes	and
T18870	1288-1293	VBN	denotes	grown
T18871	1294-1296	IN	denotes	in
T18872	1297-1303	NNP	denotes	Iscove
T18873	1303-1305	POS	denotes	's
T18874	1306-1312	NN	denotes	medium
T18875	1313-1325	VBN	denotes	supplemented
T18876	1326-1330	IN	denotes	with
T18877	1331-1347	JJ	denotes	heat-inactivated
T18878	1348-1350	CD	denotes	10
T18879	1350-1351	NN	denotes	%
T18880	1352-1357	JJ	denotes	fetal
T18881	1358-1364	JJ	denotes	bovine
T18882	1365-1370	NN	denotes	serum
T18883	1371-1374	CC	denotes	and
T18884	1375-1397	JJ	denotes	antibiotic/antimycotic
T18885	1398-1399	-LRB-	denotes	(
T18886	1399-1404	NNP	denotes	GIBCO
T18887	1404-1405	-RRB-	denotes	)
T18888	1405-1406	.	denotes	.
T18889	1407-1418	NNS	denotes	Splenocytes
T18890	1419-1427	VBD	denotes	isolated
T18891	1428-1432	IN	denotes	from
T18892	1433-1446	JJ	denotes	SCID-passaged
T18893	1447-1456	NNS	denotes	lymphomas
T18894	1457-1466	VBD	denotes	consisted
T18895	1467-1469	IN	denotes	of
T18896	1470-1472	CD	denotes	80
T18897	1472-1473	NN	denotes	%
T18898	1474-1476	CD	denotes	90
T18899	1476-1477	NN	denotes	%
T18900	1478-1479	NN	denotes	B
T18901	1480-1485	NNS	denotes	cells
T18902	1486-1488	IN	denotes	as
T18903	1489-1499	VBN	denotes	determined
T18904	1500-1502	IN	denotes	by
T18905	1503-1507	NN	denotes	flow
T18906	1508-1517	NN	denotes	cytometry
T18907	1517-1518	,	denotes	,
T18908	1519-1522	CC	denotes	and
T18909	1523-1527	VBD	denotes	were
T18910	1528-1533	RB	denotes	hence
T18911	1534-1537	RB	denotes	not
T18912	1538-1545	JJ	denotes	further
T18913	1546-1554	VBN	denotes	purified
T18914	1555-1559	IN	denotes	with
T18915	1560-1569	NN	denotes	CD19-MACS
T18916	1570-1575	NNS	denotes	beads
T18917	1575-1576	.	denotes	.
T18918	1577-1588	NNS	denotes	Splenocytes
T18919	1589-1593	VBD	denotes	were
T18920	1594-1600	VBN	denotes	seeded
T18921	1601-1603	IN	denotes	at
T18922	1604-1608	CD	denotes	1.25
T18923	1609-1610	CD	denotes	×
T18924	1611-1614	CD	denotes	106
T18925	1615-1623	NN	denotes	cells/ml
T18926	1624-1627	CC	denotes	and
T18927	1628-1633	WRB	denotes	where
T18928	1634-1644	JJ	denotes	applicable
T18929	1644-1645	,	denotes	,
T18930	1646-1657	JJ	denotes	recombinant
T18931	1658-1663	NN	denotes	mouse
T18932	1664-1667	NNP	denotes	IL4
T18933	1668-1671	VBD	denotes	was
T18934	1672-1677	VBN	denotes	added
T18935	1678-1680	IN	denotes	at
T18936	1681-1684	CD	denotes	100
T18937	1685-1690	NN	denotes	ng/ml
T18938	1690-1691	,	denotes	,
T18939	1692-1703	JJ	denotes	recombinant
T18940	1704-1709	NN	denotes	mouse
T18941	1710-1714	NNP	denotes	IL10
T18942	1715-1717	IN	denotes	at
T18943	1718-1720	CD	denotes	10
T18944	1721-1726	NN	denotes	ng/ml
T18945	1726-1727	,	denotes	,
T18946	1728-1731	CC	denotes	and
T18947	1732-1735	NN	denotes	rat
T18948	1736-1740	JJ	denotes	IgG1
T18949	1741-1751	JJ	denotes	anti-mouse
T18950	1752-1756	NNP	denotes	IL10
T18951	1757-1760	CC	denotes	and
T18952	1761-1764	VB	denotes	rat
T18953	1765-1769	JJ	denotes	IgG1
T18954	1770-1777	NN	denotes	isotype
T18955	1778-1785	NN	denotes	control
T18956	1786-1788	IN	denotes	at
T18957	1789-1791	CD	denotes	10
T18958	1792-1794	NN	denotes	μg
T18959	1794-1795	NN	denotes	/
T18960	1795-1797	NN	denotes	ml
T18961	1798-1799	-LRB-	denotes	(
T18962	1799-1802	NNP	denotes	R&D
T18963	1803-1810	NNPS	denotes	Systems
T18964	1810-1811	-RRB-	denotes	)
T18965	1811-1812	.	denotes	.
T18966	1813-1816	IN	denotes	For
T18967	1817-1821	NNP	denotes	BrdU
T18968	1822-1835	NN	denotes	incorporation
T18969	1836-1842	NNS	denotes	assays
T18970	1842-1843	,	denotes	,
T18971	1844-1855	NNS	denotes	splenocytes
T18972	1856-1860	VBD	denotes	were
T18973	1861-1867	VBN	denotes	pulsed
T18974	1868-1871	IN	denotes	for
T18975	1872-1874	CD	denotes	24
T18976	1875-1876	NN	denotes	h
T18977	1877-1881	IN	denotes	with
T18978	1882-1884	CD	denotes	10
T18979	1885-1887	NNP	denotes	μM
T18980	1888-1892	NNP	denotes	BrdU
T18981	1893-1894	CD	denotes	1
T18982	1895-1896	LS	denotes	d
T18983	1897-1909	JJS	denotes	post-harvest
T18984	1909-1910	.	denotes	.
T19315	1912-1915	NNP	denotes	MTS
T19316	1916-1921	NN	denotes	assay
T19317	1921-1922	.	denotes	.
T19318	1923-1926	DT	denotes	The
T19319	1927-1928	LS	denotes	3
T19320	1928-1929	:	denotes	-
T19321	1929-1930	-LRB-	denotes	(
T19322	1930-1931	CD	denotes	4
T19323	1931-1932	,	denotes	,
T19324	1933-1955	JJ	denotes	5-dimethylthiazol-2-yl
T19325	1955-1956	-RRB-	denotes	)
T19326	1956-1958	SYM	denotes	-5
T19327	1958-1959	:	denotes	-
T19328	1959-1960	-LRB-	denotes	(
T19329	1960-1982	JJ	denotes	3-carboxymethoxyphenyl
T19330	1982-1983	-RRB-	denotes	)
T19331	1983-1985	SYM	denotes	-2
T19332	1985-1986	:	denotes	-
T19333	1986-1987	-LRB-	denotes	(
T19334	1987-2000	JJ	denotes	4-sulfophenyl
T19335	2000-2001	-RRB-	denotes	)
T19336	2001-2003	CD	denotes	-2
T19337	2003-2016	NN	denotes	H-tetrazolium
T19338	2017-2022	JJ	denotes	inner
T19339	2023-2027	NN	denotes	salt
T19340	2028-2029	-LRB-	denotes	(
T19341	2029-2032	NNP	denotes	MTS
T19342	2032-2033	-RRB-	denotes	)
T19343	2034-2038	NN	denotes	cell
T19344	2039-2065	NN	denotes	cytotoxicity/proliferation
T19345	2066-2072	NNS	denotes	assays
T19346	2073-2077	VBD	denotes	were
T19347	2078-2087	VBN	denotes	performed
T19348	2088-2093	VBG	denotes	using
T19349	2094-2097	DT	denotes	the
T19350	2098-2107	NNP	denotes	CellTiter
T19351	2108-2110	CD	denotes	96
T19352	2111-2118	JJ	denotes	aqueous
T19353	2119-2131	JJ	denotes	one-solution
T19354	2132-2136	NN	denotes	cell
T19355	2137-2150	NN	denotes	proliferation
T19356	2151-2156	NN	denotes	assay
T19357	2157-2158	-LRB-	denotes	(
T19358	2158-2165	NNP	denotes	Promega
T19359	2165-2166	-RRB-	denotes	)
T19360	2166-2167	,	denotes	,
T19361	2168-2177	VBG	denotes	according
T19362	2178-2180	TO	denotes	to
T19363	2181-2193	NN	denotes	manufacturer
T19364	2193-2195	POS	denotes	's
T19365	2196-2208	NNS	denotes	instructions
T19366	2208-2209	.	denotes	.
T19367	2210-2213	IN	denotes	For
T19368	2214-2217	CD	denotes	IL4
T19369	2218-2225	NNS	denotes	studies
T19370	2225-2226	,	denotes	,
T19371	2227-2238	NNS	denotes	splenocytes
T19372	2239-2243	VBD	denotes	were
T19373	2244-2252	VBN	denotes	cultured
T19374	2253-2256	IN	denotes	for
T19375	2257-2258	CD	denotes	3
T19376	2259-2260	LS	denotes	d
T19377	2261-2263	IN	denotes	in
T19378	2264-2267	DT	denotes	the
T19379	2268-2276	NN	denotes	presence
T19380	2277-2279	CC	denotes	or
T19381	2280-2287	NN	denotes	absence
T19382	2288-2290	IN	denotes	of
T19383	2291-2294	CD	denotes	100
T19384	2295-2300	JJ	denotes	ng/ml
T19385	2301-2304	NN	denotes	IL4
T19386	2304-2305	.	denotes	.
T19387	2306-2311	NNS	denotes	Cells
T19388	2312-2316	VBD	denotes	were
T19389	2317-2323	VBN	denotes	seeded
T19390	2324-2326	IN	denotes	on
T19391	2327-2330	NN	denotes	day
T19392	2331-2332	CD	denotes	3
T19393	2333-2335	IN	denotes	in
T19394	2336-2346	NN	denotes	triplicate
T19395	2347-2349	IN	denotes	in
T19396	2350-2351	DT	denotes	a
T19397	2352-2359	JJ	denotes	96-well
T19398	2360-2365	NN	denotes	plate
T19399	2366-2368	IN	denotes	at
T19400	2369-2372	CD	denotes	2.5
T19401	2373-2374	CD	denotes	×
T19402	2375-2378	CD	denotes	106
T19403	2379-2387	NN	denotes	cells/ml
T19404	2388-2390	IN	denotes	at
T19405	2391-2394	CD	denotes	100
T19406	2395-2397	NN	denotes	μl
T19407	2398-2401	IN	denotes	per
T19408	2402-2406	RB	denotes	well
T19409	2406-2407	.	denotes	.
T19410	2408-2411	NNP	denotes	MTS
T19411	2412-2419	NN	denotes	reagent
T19412	2420-2423	VBD	denotes	was
T19413	2424-2429	VBN	denotes	added
T19414	2430-2433	IN	denotes	for
T19415	2434-2435	CD	denotes	4
T19416	2436-2437	NN	denotes	h
T19417	2438-2441	CC	denotes	and
T19418	2442-2452	NN	denotes	absorbance
T19419	2453-2456	VBD	denotes	was
T19420	2457-2461	VBN	denotes	read
T19421	2462-2464	IN	denotes	at
T19422	2465-2468	CD	denotes	540
T19423	2469-2471	NN	denotes	nm
T19424	2471-2472	:	denotes	;
T19425	2473-2479	NNS	denotes	values
T19426	2480-2487	VBN	denotes	plotted
T19427	2488-2492	VBD	denotes	were
T19428	2493-2503	VBN	denotes	subtracted
T19429	2504-2508	IN	denotes	from
T19430	2509-2515	NNS	denotes	blanks
T19431	2515-2516	.	denotes	.
T19432	2517-2520	IN	denotes	For
T19433	2521-2535	NN	denotes	neutralization
T19434	2536-2542	NNS	denotes	assays
T19435	2542-2543	,	denotes	,
T19436	2544-2555	NNS	denotes	splenocytes
T19437	2556-2560	VBD	denotes	were
T19438	2561-2567	VBN	denotes	seeded
T19439	2568-2570	IN	denotes	at
T19440	2571-2572	CD	denotes	5
T19441	2573-2574	CD	denotes	×
T19442	2575-2578	CD	denotes	106
T19443	2579-2587	NN	denotes	cells/ml
T19444	2588-2590	IN	denotes	at
T19445	2591-2594	CD	denotes	100
T19446	2595-2597	NN	denotes	μl
T19447	2598-2601	IN	denotes	per
T19448	2602-2606	RB	denotes	well
T19449	2607-2609	IN	denotes	on
T19450	2610-2613	NN	denotes	day
T19451	2614-2616	IN	denotes	of
T19452	2617-2624	NN	denotes	harvest
T19453	2625-2628	CC	denotes	and
T19454	2629-2632	NNP	denotes	IL4
T19455	2632-2633	,	denotes	,
T19456	2634-2637	NN	denotes	rat
T19457	2638-2642	NNP	denotes	IgG1
T19458	2643-2653	JJ	denotes	anti-mouse
T19459	2654-2657	NNP	denotes	IL4
T19460	2657-2658	,	denotes	,
T19461	2659-2661	CC	denotes	or
T19462	2662-2665	NN	denotes	rat
T19463	2666-2670	JJ	denotes	IgG1
T19464	2671-2678	NN	denotes	isotype
T19465	2679-2686	NN	denotes	control
T19466	2687-2688	-LRB-	denotes	(
T19467	2688-2691	NNP	denotes	R&D
T19468	2692-2699	NNPS	denotes	Systems
T19469	2699-2700	-RRB-	denotes	)
T19470	2701-2705	VBD	denotes	were
T19471	2706-2711	VBN	denotes	added
T19472	2712-2714	IN	denotes	at
T19473	2715-2718	DT	denotes	the
T19474	2719-2733	NNS	denotes	concentrations
T19475	2734-2743	VBD	denotes	indicated
T19476	2744-2746	IN	denotes	in
T19477	2747-2750	DT	denotes	the
T19478	2751-2758	NNS	denotes	figures
T19479	2758-2759	.	denotes	.
T19480	2760-2768	NNS	denotes	Cultures
T19481	2769-2773	VBD	denotes	were
T19482	2774-2780	VBN	denotes	pulsed
T19483	2781-2784	IN	denotes	for
T19484	2785-2786	CD	denotes	4
T19485	2787-2788	NN	denotes	h
T19486	2789-2793	IN	denotes	with
T19487	2794-2797	NNP	denotes	MTS
T19488	2798-2805	NN	denotes	reagent
T19489	2806-2807	CD	denotes	1
T19490	2808-2809	LS	denotes	d
T19491	2810-2822	NN	denotes	post-seeding
T19492	2822-2823	.	denotes	.
T19493	2824-2827	IN	denotes	For
T19494	2828-2837	JJ	denotes	inhibitor
T19495	2838-2845	NNS	denotes	studies
T19496	2845-2846	,	denotes	,
T19497	2847-2858	NNS	denotes	splenocytes
T19498	2859-2863	VBD	denotes	were
T19499	2864-2870	VBN	denotes	seeded
T19500	2871-2873	IN	denotes	at
T19501	2874-2875	CD	denotes	1
T19502	2876-2877	CD	denotes	×
T19503	2878-2881	CD	denotes	107
T19504	2882-2890	NN	denotes	cells/ml
T19505	2891-2893	IN	denotes	at
T19506	2894-2897	CD	denotes	100
T19507	2898-2900	NN	denotes	μl
T19508	2901-2904	IN	denotes	per
T19509	2905-2909	RB	denotes	well
T19510	2910-2912	IN	denotes	on
T19511	2913-2916	NN	denotes	day
T19512	2917-2919	IN	denotes	of
T19513	2920-2927	NN	denotes	harvest
T19514	2927-2928	,	denotes	,
T19515	2929-2932	CC	denotes	and
T19516	2933-2943	NNS	denotes	inhibitors
T19517	2944-2948	VBD	denotes	were
T19518	2949-2954	VBN	denotes	added
T19519	2955-2957	IN	denotes	at
T19520	2958-2961	DT	denotes	the
T19521	2962-2976	NNS	denotes	concentrations
T19522	2977-2986	VBD	denotes	indicated
T19523	2987-2989	IN	denotes	in
T19524	2990-2993	DT	denotes	the
T19525	2994-3001	NNS	denotes	figures
T19526	3001-3002	.	denotes	.
T19527	3003-3006	DT	denotes	The
T19528	3007-3017	NNS	denotes	inhibitors
T19529	3018-3023	NNP	denotes	BAY11
T19530	3024-3028	CD	denotes	7085
T19531	3028-3029	,	denotes	,
T19532	3030-3039	NN	denotes	rapamycin
T19533	3039-3040	,	denotes	,
T19534	3041-3052	NN	denotes	triciribine
T19535	3052-3053	,	denotes	,
T19536	3054-3059	NNP	denotes	U0126
T19537	3059-3060	,	denotes	,
T19538	3061-3069	NNP	denotes	SB202190
T19539	3069-3070	,	denotes	,
T19540	3071-3074	CC	denotes	and
T19541	3075-3087	NN	denotes	cucurbitacin
T19542	3088-3089	PRP	denotes	I
T19543	3090-3094	VBD	denotes	were
T19544	3095-3104	VBN	denotes	purchased
T19545	3105-3109	IN	denotes	from
T19546	3110-3113	NNP	denotes	EMD
T19547	3114-3125	NNPS	denotes	Biosciences
T19548	3125-3126	.	denotes	.
T19549	3127-3130	IN	denotes	For
T19550	3131-3144	JJ	denotes	non-malignant
T19551	3145-3155	NN	denotes	splenocyte
T19552	3156-3164	NNS	denotes	cultures
T19553	3164-3165	,	denotes	,
T19554	3166-3167	NNP	denotes	B
T19555	3168-3172	NN	denotes	cell
T19556	3173-3183	NN	denotes	activation
T19557	3184-3187	VBD	denotes	was
T19558	3188-3195	VBN	denotes	induced
T19559	3196-3200	IN	denotes	with
T19560	3201-3203	CD	denotes	10
T19561	3204-3206	NN	denotes	μg
T19562	3206-3207	NN	denotes	/
T19563	3207-3209	NN	denotes	ml
T19564	3210-3212	IN	denotes	of
T19565	3213-3217	NN	denotes	goat
T19566	3218-3219	NN	denotes	F
T19567	3219-3220	-LRB-	denotes	(
T19568	3220-3222	NN	denotes	ab
T19569	3222-3223	''	denotes	'
T19570	3223-3224	-RRB-	denotes	)
T19571	3225-3235	JJ	denotes	anti-mouse
T19572	3236-3239	NNP	denotes	IgM
T19573	3240-3241	-LRB-	denotes	(
T19574	3241-3248	NNP	denotes	Jackson
T19575	3249-3263	NNP	denotes	ImmunoResearch
T19576	3263-3264	-RRB-	denotes	)
T19577	3264-3265	.	denotes	.
T19578	3266-3274	NNS	denotes	Cultures
T19579	3275-3279	VBD	denotes	were
T19580	3280-3286	VBN	denotes	pulsed
T19581	3287-3290	IN	denotes	for
T19582	3291-3292	CD	denotes	4
T19583	3293-3294	NN	denotes	h
T19584	3295-3299	IN	denotes	with
T19585	3300-3303	NNP	denotes	MTS
T19586	3304-3311	NN	denotes	reagent
T19587	3312-3313	CD	denotes	1
T19588	3314-3315	LS	denotes	d
T19589	3316-3328	NN	denotes	post-seeding
T19590	3328-3329	.	denotes	.
T19824	3331-3351	NNP	denotes	Immunohistochemistry
T19825	3351-3352	.	denotes	.
T19826	3353-3370	JJ	denotes	Paraffin-embedded
T19827	3371-3377	JJ	denotes	spleen
T19828	3378-3386	NNS	denotes	sections
T19829	3387-3391	VBD	denotes	were
T19830	3392-3406	VBN	denotes	deparaffinized
T19831	3407-3409	IN	denotes	in
T19832	3410-3420	NNP	denotes	Histoclear
T19833	3421-3422	-LRB-	denotes	(
T19834	3422-3430	NNP	denotes	National
T19835	3431-3442	NNPS	denotes	Diagnostics
T19836	3442-3443	-RRB-	denotes	)
T19837	3444-3447	CC	denotes	and
T19838	3448-3458	VBD	denotes	rehydrated
T19839	3459-3461	IN	denotes	in
T19840	3462-3468	JJ	denotes	graded
T19841	3469-3476	NN	denotes	ethanol
T19842	3476-3477	.	denotes	.
T19843	3478-3486	NNS	denotes	Sections
T19844	3487-3491	VBD	denotes	were
T19845	3492-3499	NN	denotes	antigen
T19846	3500-3509	VBN	denotes	retrieved
T19847	3510-3512	IN	denotes	by
T19848	3513-3524	NN	denotes	microwaving
T19849	3525-3527	IN	denotes	in
T19850	3528-3535	JJ	denotes	citrate
T19851	3536-3542	NN	denotes	buffer
T19852	3543-3544	-LRB-	denotes	(
T19853	3544-3546	NNP	denotes	pH
T19854	3547-3550	CD	denotes	6.0
T19855	3550-3551	-RRB-	denotes	)
T19856	3552-3555	IN	denotes	for
T19857	3556-3558	CD	denotes	15
T19858	3559-3562	NN	denotes	min
T19859	3563-3564	-LRB-	denotes	(
T19860	3564-3568	CD	denotes	LMP1
T19861	3569-3577	NN	denotes	staining
T19862	3577-3578	-RRB-	denotes	)
T19863	3579-3582	CC	denotes	and
T19864	3583-3585	CD	denotes	10
T19865	3586-3589	NN	denotes	min
T19866	3590-3591	-LRB-	denotes	(
T19867	3591-3597	JJ	denotes	pStat3
T19868	3598-3606	NN	denotes	staining
T19869	3606-3607	-RRB-	denotes	)
T19870	3607-3608	.	denotes	.
T19871	3609-3612	IN	denotes	For
T19872	3613-3617	CD	denotes	LMP1
T19873	3618-3626	NN	denotes	staining
T19874	3626-3627	,	denotes	,
T19875	3628-3636	NNS	denotes	sections
T19876	3637-3641	VBD	denotes	were
T19877	3642-3649	VBN	denotes	blocked
T19878	3650-3654	IN	denotes	with
T19879	3655-3656	CD	denotes	1
T19880	3656-3657	NN	denotes	%
T19881	3658-3661	NNP	denotes	BSA
T19882	3662-3665	CC	denotes	and
T19883	3666-3669	CD	denotes	0.1
T19884	3669-3670	NN	denotes	%
T19885	3671-3675	JJ	denotes	cold
T19886	3676-3680	NN	denotes	fish
T19887	3681-3685	NN	denotes	skin
T19888	3686-3693	NN	denotes	gelatin
T19889	3694-3702	VBD	denotes	followed
T19890	3703-3707	IN	denotes	with
T19891	3708-3727	NNP	denotes	streptavidin/biotin
T19892	3728-3733	NN	denotes	block
T19893	3734-3735	-LRB-	denotes	(
T19894	3735-3741	NNP	denotes	Vector
T19895	3742-3746	NNPS	denotes	Labs
T19896	3746-3747	-RRB-	denotes	)
T19897	3747-3748	.	denotes	.
T19898	3749-3752	NN	denotes	Rat
T19899	3753-3756	NNP	denotes	IgG
T19900	3757-3766	NNP	denotes	anti-LMP1
T19901	3767-3768	-LRB-	denotes	(
T19902	3768-3774	NNS	denotes	clones
T19903	3775-3778	NNP	denotes	8G3
T19904	3779-3782	CC	denotes	and
T19905	3783-3786	NNP	denotes	1G6
T19906	3786-3787	,	denotes	,
T19907	3788-3796	NNP	denotes	Ascenion
T19908	3796-3797	-RRB-	denotes	)
T19909	3798-3802	VBD	denotes	were
T19910	3803-3807	VBN	denotes	used
T19911	3808-3810	IN	denotes	at
T19912	3811-3815	CD	denotes	1:10
T19913	3816-3824	NN	denotes	dilution
T19914	3825-3829	IN	denotes	from
T19915	3830-3836	NN	denotes	tissue
T19916	3837-3844	NN	denotes	culture
T19917	3845-3857	NNS	denotes	supernatants
T19918	3857-3858	,	denotes	,
T19919	3859-3867	VBD	denotes	followed
T19920	3868-3872	IN	denotes	with
T19921	3873-3874	CD	denotes	8
T19922	3875-3877	NN	denotes	μg
T19923	3877-3878	NN	denotes	/
T19924	3878-3880	NN	denotes	ml
T19925	3881-3893	VBD	denotes	biotinylated
T19926	3894-3899	NN	denotes	mouse
T19927	3900-3901	NN	denotes	F
T19928	3901-3902	-LRB-	denotes	(
T19929	3902-3904	NN	denotes	ab
T19930	3904-3905	''	denotes	'
T19931	3905-3906	-RRB-	denotes	)
T19932	3907-3915	JJ	denotes	anti-rat
T19933	3916-3919	NNP	denotes	IgG
T19934	3920-3921	-LRB-	denotes	(
T19935	3921-3924	NNP	denotes	H+L
T19936	3924-3925	-RRB-	denotes	)
T19937	3926-3938	JJ	denotes	pre-adsorbed
T19938	3939-3941	TO	denotes	to
T19939	3942-3947	NN	denotes	mouse
T19940	3948-3953	NN	denotes	serum
T19941	3954-3955	-LRB-	denotes	(
T19942	3955-3962	NNP	denotes	Jackson
T19943	3963-3977	NNP	denotes	ImmunoResearch
T19944	3977-3978	-RRB-	denotes	)
T19945	3979-3982	CC	denotes	and
T19946	3983-3984	CD	denotes	2
T19947	3985-3987	NN	denotes	μg
T19948	3987-3988	NN	denotes	/
T19949	3988-3990	NN	denotes	ml
T19950	3991-4012	JJ	denotes	streptavidin-alkaline
T19951	4013-4024	NN	denotes	phosphatase
T19952	4025-4034	NN	denotes	conjugate
T19953	4035-4036	-LRB-	denotes	(
T19954	4036-4043	NNP	denotes	Jackson
T19955	4044-4058	NNP	denotes	ImmunoResearch
T19956	4058-4059	-RRB-	denotes	)
T19957	4059-4060	.	denotes	.
T19958	4061-4067	NNS	denotes	Stains
T19959	4068-4072	VBD	denotes	were
T19960	4073-4082	VBN	denotes	developed
T19961	4083-4087	IN	denotes	with
T19962	4088-4096	NNP	denotes	BCIP/NBT
T19963	4097-4100	CC	denotes	and
T19964	4101-4115	VBN	denotes	counterstained
T19965	4116-4118	IN	denotes	in
T19966	4119-4126	NNP	denotes	Nuclear
T19967	4127-4131	NNP	denotes	Fast
T19968	4132-4135	NNP	denotes	Red
T19969	4136-4137	-LRB-	denotes	(
T19970	4137-4141	NNP	denotes	Dako
T19971	4141-4142	-RRB-	denotes	)
T19972	4142-4143	.	denotes	.
T19973	4144-4157	NN	denotes	Phospho-Stat3
T19974	4158-4161	VBD	denotes	was
T19975	4162-4170	VBN	denotes	detected
T19976	4171-4175	IN	denotes	with
T19977	4176-4177	CD	denotes	4
T19978	4178-4180	NN	denotes	μg
T19979	4180-4181	NN	denotes	/
T19980	4181-4183	NN	denotes	ml
T19981	4184-4186	IN	denotes	of
T19982	4187-4193	JJ	denotes	pStat3
T19983	4194-4202	NN	denotes	antibody
T19984	4203-4204	-LRB-	denotes	(
T19985	4204-4210	NNS	denotes	Tyr705
T19986	4210-4211	,	denotes	,
T19987	4212-4216	NNP	denotes	Cell
T19988	4217-4226	NNP	denotes	Signaling
T19989	4226-4227	-RRB-	denotes	)
T19990	4228-4231	CC	denotes	and
T19991	4232-4240	VBN	denotes	detected
T19992	4241-4245	IN	denotes	with
T19993	4246-4257	JJ	denotes	anti-rabbit
T19994	4258-4266	NNP	denotes	Poly-HRP
T19995	4267-4270	NNP	denotes	IHC
T19996	4271-4280	NN	denotes	detection
T19997	4281-4284	NN	denotes	kit
T19998	4285-4286	-LRB-	denotes	(
T19999	4286-4294	NNP	denotes	Chemicon
T20000	4294-4295	-RRB-	denotes	)
T20001	4295-4296	.	denotes	.
T20169	4298-4302	NN	denotes	Flow
T20170	4303-4312	NN	denotes	cytometry
T20171	4312-4313	.	denotes	.
T20172	4314-4317	CD	denotes	One
T20173	4318-4325	CD	denotes	million
T20174	4326-4337	NNS	denotes	splenocytes
T20175	4338-4342	VBD	denotes	were
T20176	4343-4350	VBN	denotes	stained
T20177	4351-4355	IN	denotes	with
T20178	4356-4359	DT	denotes	the
T20179	4360-4371	JJ	denotes	appropriate
T20180	4372-4379	JJ	denotes	primary
T20181	4380-4388	NN	denotes	antibody
T20182	4389-4401	JJ	denotes	unconjugated
T20183	4402-4404	CC	denotes	or
T20184	4405-4415	VBN	denotes	conjugated
T20185	4416-4418	TO	denotes	to
T20186	4419-4423	NNP	denotes	FITC
T20187	4423-4424	,	denotes	,
T20188	4425-4427	NNP	denotes	PE
T20189	4427-4428	,	denotes	,
T20190	4429-4431	CC	denotes	or
T20191	4432-4435	NNP	denotes	APC
T20192	4436-4443	VBN	denotes	diluted
T20193	4444-4446	IN	denotes	in
T20194	4447-4452	VB	denotes	stain
T20195	4453-4459	NN	denotes	buffer
T20196	4460-4461	-LRB-	denotes	(
T20197	4461-4464	NNP	denotes	PBS
T20198	4465-4469	IN	denotes	with
T20199	4470-4471	CD	denotes	3
T20200	4471-4472	NN	denotes	%
T20201	4473-4476	NNP	denotes	FBS
T20202	4476-4477	-RRB-	denotes	)
T20203	4477-4478	.	denotes	.
T20204	4479-4482	IN	denotes	For
T20205	4483-4487	NNP	denotes	BrdU
T20206	4488-4497	NN	denotes	detection
T20207	4497-4498	,	denotes	,
T20208	4499-4502	DT	denotes	the
T20209	4503-4512	NNP	denotes	FITC-BrdU
T20210	4513-4517	NN	denotes	flow
T20211	4518-4521	NN	denotes	kit
T20212	4522-4525	VBD	denotes	was
T20213	4526-4530	VBN	denotes	used
T20214	4531-4533	IN	denotes	as
T20215	4534-4544	VBN	denotes	instructed
T20216	4545-4547	IN	denotes	by
T20217	4548-4551	DT	denotes	the
T20218	4552-4564	NN	denotes	manufacturer
T20219	4565-4566	-LRB-	denotes	(
T20220	4566-4568	NNP	denotes	BD
T20221	4569-4579	NNP	denotes	Bioscience
T20222	4579-4580	-RRB-	denotes	)
T20223	4580-4581	.	denotes	.
T20224	4582-4589	RB	denotes	Briefly
T20225	4589-4590	,	denotes	,
T20226	4591-4596	NNS	denotes	cells
T20227	4597-4601	VBD	denotes	were
T20228	4602-4609	VBN	denotes	exposed
T20229	4610-4612	TO	denotes	to
T20230	4613-4616	NNP	denotes	EMA
T20231	4617-4618	-LRB-	denotes	(
T20232	4618-4627	NNP	denotes	Molecular
T20233	4628-4634	NNP	denotes	Probes
T20234	4634-4635	-RRB-	denotes	)
T20235	4636-4639	IN	denotes	for
T20236	4640-4649	NN	denotes	exclusion
T20237	4650-4652	IN	denotes	of
T20238	4653-4657	JJ	denotes	dead
T20239	4658-4663	NNS	denotes	cells
T20240	4663-4664	,	denotes	,
T20241	4665-4672	VBN	denotes	stained
T20242	4673-4676	IN	denotes	for
T20243	4677-4684	NN	denotes	surface
T20244	4685-4693	NNS	denotes	antigens
T20245	4693-4694	,	denotes	,
T20246	4695-4700	VBN	denotes	fixed
T20247	4701-4703	IN	denotes	in
T20248	4704-4720	NN	denotes	paraformaldehyde
T20249	4720-4721	,	denotes	,
T20250	4722-4725	CC	denotes	and
T20251	4726-4739	VBN	denotes	permeabilized
T20252	4740-4744	IN	denotes	with
T20253	4745-4752	NN	denotes	saponin
T20254	4752-4753	.	denotes	.
T20255	4754-4756	TO	denotes	To
T20256	4757-4763	VB	denotes	expose
T20257	4764-4768	NNP	denotes	BrdU
T20258	4769-4777	NNS	denotes	epitopes
T20259	4777-4778	,	denotes	,
T20260	4779-4784	NNS	denotes	cells
T20261	4785-4789	VBD	denotes	were
T20262	4790-4797	VBN	denotes	treated
T20263	4798-4802	IN	denotes	with
T20264	4803-4808	NNP	denotes	Dnase
T20265	4809-4812	CC	denotes	and
T20266	4813-4820	VBN	denotes	stained
T20267	4821-4825	IN	denotes	with
T20268	4826-4841	JJ	denotes	FITC-conjugated
T20269	4842-4851	JJ	denotes	anti-BrdU
T20270	4852-4860	NN	denotes	antibody
T20271	4860-4861	.	denotes	.
T20272	4862-4866	NN	denotes	Flow
T20273	4867-4876	NN	denotes	cytometry
T20274	4877-4880	VBD	denotes	was
T20275	4881-4890	VBN	denotes	performed
T20276	4891-4893	IN	denotes	on
T20277	4894-4905	NNP	denotes	FACScalibur
T20278	4906-4911	VBG	denotes	using
T20279	4912-4915	DT	denotes	the
T20280	4916-4925	NNP	denotes	CellQuest
T20281	4926-4933	NN	denotes	program
T20282	4934-4935	-LRB-	denotes	(
T20283	4935-4941	NNP	denotes	Becton
T20284	4942-4951	NNP	denotes	Dickinson
T20285	4951-4952	-RRB-	denotes	)
T20286	4952-4953	.	denotes	.
T20287	4954-4961	JJ	denotes	Further
T20288	4962-4970	NN	denotes	analysis
T20289	4971-4974	VBD	denotes	was
T20290	4975-4984	VBN	denotes	conducted
T20291	4985-4987	IN	denotes	on
T20292	4988-4991	DT	denotes	the
T20293	4992-4998	NNP	denotes	Summit
T20294	4999-5001	CD	denotes	v4
T20295	5001-5003	CD	denotes	.2
T20296	5004-5011	NN	denotes	program
T20297	5012-5013	-LRB-	denotes	(
T20298	5013-5017	NNP	denotes	Dako
T20299	5017-5018	-RRB-	denotes	)
T20300	5018-5019	.	denotes	.
T20534	5021-5026	JJ	denotes	Rnase
T20535	5027-5037	NN	denotes	protection
T20536	5038-5043	NN	denotes	assay
T20537	5043-5044	.	denotes	.
T20538	5045-5050	JJ	denotes	Total
T20539	5051-5054	NNP	denotes	RNA
T20540	5055-5058	VBD	denotes	was
T20541	5059-5067	VBN	denotes	isolated
T20542	5068-5072	IN	denotes	from
T20543	5073-5077	CD	denotes	CD19
T20544	5077-5078	CD	denotes	+
T20545	5079-5092	JJ	denotes	MACS-purified
T20546	5093-5094	NNP	denotes	B
T20547	5095-5100	NNS	denotes	cells
T20548	5101-5106	VBG	denotes	using
T20549	5107-5110	DT	denotes	the
T20550	5111-5117	NNP	denotes	Rneasy
T20551	5118-5122	NN	denotes	midi
T20552	5123-5135	NN	denotes	purification
T20553	5136-5139	NN	denotes	kit
T20554	5140-5144	IN	denotes	with
T20555	5145-5150	NNP	denotes	Dnase
T20556	5151-5160	NN	denotes	treatment
T20557	5160-5161	,	denotes	,
T20558	5162-5171	VBG	denotes	according
T20559	5172-5174	TO	denotes	to
T20560	5175-5178	DT	denotes	the
T20561	5179-5191	NN	denotes	manufacturer
T20562	5191-5193	POS	denotes	's
T20563	5194-5206	NNS	denotes	instructions
T20564	5207-5208	-LRB-	denotes	(
T20565	5208-5214	NNP	denotes	Qiagen
T20566	5214-5215	-RRB-	denotes	)
T20567	5215-5216	.	denotes	.
T20568	5217-5220	DT	denotes	The
T20569	5221-5226	JJ	denotes	mCK-1
T20570	5227-5232	NN	denotes	probe
T20571	5233-5241	NN	denotes	template
T20572	5242-5245	NN	denotes	set
T20573	5246-5247	-LRB-	denotes	(
T20574	5247-5249	NNP	denotes	BD
T20575	5250-5261	NNPS	denotes	Biosciences
T20576	5261-5262	-RRB-	denotes	)
T20577	5263-5266	VBD	denotes	was
T20578	5267-5274	VBN	denotes	labeled
T20579	5275-5280	VBG	denotes	using
T20580	5281-5284	DT	denotes	the
T20581	5285-5287	NNP	denotes	In
T20582	5288-5293	NNP	denotes	Vitro
T20583	5294-5307	NNP	denotes	Transcription
T20584	5308-5311	NNP	denotes	Kit
T20585	5312-5321	VBG	denotes	according
T20586	5322-5324	TO	denotes	to
T20587	5325-5328	DT	denotes	the
T20588	5329-5341	NN	denotes	manufacturer
T20589	5341-5343	POS	denotes	's
T20590	5344-5356	NNS	denotes	instructions
T20591	5357-5358	-LRB-	denotes	(
T20592	5358-5360	NNP	denotes	BD
T20593	5361-5372	NNPS	denotes	Biosciences
T20594	5372-5373	-RRB-	denotes	)
T20595	5373-5374	.	denotes	.
T20596	5375-5382	RB	denotes	Briefly
T20597	5382-5383	,	denotes	,
T20598	5384-5386	CD	denotes	50
T20599	5387-5389	NN	denotes	ng
T20600	5390-5392	IN	denotes	of
T20601	5393-5398	JJ	denotes	mCK-1
T20602	5399-5404	NN	denotes	probe
T20603	5405-5408	NN	denotes	set
T20604	5409-5412	VBD	denotes	was
T20605	5413-5420	VBN	denotes	labeled
T20606	5421-5425	IN	denotes	with
T20607	5426-5427	NNP	denotes	[
T20608	5427-5432	NNP	denotes	α-32P
T20609	5432-5433	NNP	denotes	]
T20610	5433-5436	NNP	denotes	UTP
T20611	5437-5442	VBG	denotes	using
T20612	5443-5445	CD	denotes	T7
T20613	5446-5449	NNP	denotes	RNA
T20614	5450-5460	NN	denotes	polymerase
T20615	5461-5464	CC	denotes	and
T20616	5465-5473	VBD	denotes	purified
T20617	5474-5479	VBG	denotes	using
T20618	5480-5488	NNP	denotes	Sephadex
T20619	5489-5493	NNP	denotes	G-50
T20620	5494-5501	NNS	denotes	columns
T20621	5502-5503	-LRB-	denotes	(
T20622	5503-5510	NNP	denotes	NucAway
T20623	5511-5515	NNP	denotes	Spin
T20624	5516-5522	NN	denotes	column
T20625	5522-5523	,	denotes	,
T20626	5524-5530	NNP	denotes	Ambion
T20627	5530-5531	-RRB-	denotes	)
T20628	5531-5532	.	denotes	.
T20629	5533-5536	DT	denotes	The
T20630	5537-5544	VBN	denotes	labeled
T20631	5545-5550	NN	denotes	probe
T20632	5551-5554	VBD	denotes	was
T20633	5555-5566	VBN	denotes	quantitated
T20634	5567-5572	VBG	denotes	using
T20635	5573-5574	DT	denotes	a
T20636	5575-5588	NN	denotes	scintillation
T20637	5589-5596	RB	denotes	counter
T20638	5596-5597	,	denotes	,
T20639	5598-5601	CC	denotes	and
T20640	5602-5603	CD	denotes	6
T20641	5604-5605	NN	denotes	×
T20642	5606-5609	CD	denotes	105
T20643	5610-5619	NN	denotes	cherenkov
T20644	5620-5623	NN	denotes	cpm
T20645	5624-5627	VBD	denotes	was
T20646	5628-5632	VBN	denotes	used
T20647	5633-5635	TO	denotes	to
T20648	5636-5645	VB	denotes	hybridize
T20649	5646-5648	TO	denotes	to
T20650	5649-5650	CD	denotes	4
T20651	5651-5653	NN	denotes	μg
T20652	5654-5656	IN	denotes	of
T20653	5657-5662	JJ	denotes	total
T20654	5663-5666	NNP	denotes	RNA
T20655	5667-5672	VBG	denotes	using
T20656	5673-5676	DT	denotes	the
T20657	5677-5680	NNP	denotes	RPA
T20658	5681-5684	NN	denotes	kit
T20659	5685-5686	-LRB-	denotes	(
T20660	5686-5688	NNP	denotes	BD
T20661	5689-5700	NNPS	denotes	Biosciences
T20662	5700-5701	-RRB-	denotes	)
T20663	5701-5702	.	denotes	.
T20664	5703-5710	NNS	denotes	Samples
T20665	5711-5715	VBD	denotes	were
T20666	5716-5725	VBN	denotes	denatured
T20667	5726-5728	IN	denotes	at
T20668	5729-5731	CD	denotes	90
T20669	5732-5733	CD	denotes	°
T20670	5733-5734	NNP	denotes	C
T20671	5735-5738	CC	denotes	and
T20672	5739-5749	VBN	denotes	hybridized
T20673	5750-5752	IN	denotes	at
T20674	5753-5755	CD	denotes	56
T20675	5756-5757	CD	denotes	°
T20676	5757-5758	NNP	denotes	C
T20677	5759-5768	JJ	denotes	overnight
T20678	5768-5769	.	denotes	.
T20679	5770-5785	JJ	denotes	Single-stranded
T20680	5786-5789	NNP	denotes	RNA
T20681	5790-5793	VBD	denotes	was
T20682	5794-5802	VBN	denotes	digested
T20683	5803-5807	IN	denotes	with
T20684	5808-5809	DT	denotes	a
T20685	5810-5817	NN	denotes	mixture
T20686	5818-5820	IN	denotes	of
T20687	5821-5826	NNP	denotes	Rnase
T20688	5827-5828	NNP	denotes	A
T20689	5829-5832	CC	denotes	and
T20690	5833-5835	NNP	denotes	T1
T20691	5835-5836	,	denotes	,
T20692	5837-5840	CC	denotes	and
T20693	5841-5853	VBD	denotes	precipitated
T20694	5854-5859	VBG	denotes	using
T20695	5860-5871	NN	denotes	isopropanol
T20696	5871-5872	.	denotes	.
T20697	5873-5882	VBN	denotes	Protected
T20698	5883-5889	NNS	denotes	probes
T20699	5890-5894	VBD	denotes	were
T20700	5895-5903	VBN	denotes	resolved
T20701	5904-5906	IN	denotes	on
T20702	5907-5908	DT	denotes	a
T20703	5909-5919	VBG	denotes	denaturing
T20704	5920-5924	CD	denotes	4.75
T20705	5924-5925	NN	denotes	%
T20706	5926-5936	NN	denotes	acrylamide
T20707	5937-5940	NN	denotes	gel
T20708	5940-5941	,	denotes	,
T20709	5942-5947	VBD	denotes	dried
T20710	5947-5948	,	denotes	,
T20711	5949-5952	CC	denotes	and
T20712	5953-5959	VBD	denotes	imaged
T20713	5960-5965	VBG	denotes	using
T20714	5966-5967	DT	denotes	a
T20715	5968-5982	NNP	denotes	phosphorImager
T20716	5983-5984	-LRB-	denotes	(
T20717	5984-5993	NNP	denotes	Molecular
T20718	5994-6002	NNP	denotes	Dynamics
T20719	6002-6003	-RRB-	denotes	)
T20720	6003-6004	.	denotes	.
T20721	6005-6017	NNP	denotes	Densitometry
T20722	6018-6021	VBD	denotes	was
T20723	6022-6031	VBN	denotes	performed
T20724	6032-6037	VBG	denotes	using
T20725	6038-6041	DT	denotes	the
T20726	6042-6052	NNP	denotes	ImageQuant
T20727	6053-6055	NNP	denotes	TL
T20728	6056-6061	CD	denotes	v2005
T20729	6062-6070	NN	denotes	software
T20730	6071-6072	-LRB-	denotes	(
T20731	6072-6074	NNP	denotes	GE
T20732	6075-6085	NNP	denotes	Healthcare
T20733	6085-6086	-RRB-	denotes	)
T20734	6086-6087	.	denotes	.
T20886	6089-6092	NNP	denotes	PCR
T20887	6093-6101	NN	denotes	analysis
T20888	6102-6104	IN	denotes	of
T20889	6105-6106	NN	denotes	κ
T20890	6107-6112	NN	denotes	chain
T20891	6113-6126	NN	denotes	rearrangement
T20892	6126-6127	.	denotes	.
T20893	6128-6131	NNP	denotes	DNA
T20894	6132-6135	VBD	denotes	was
T20895	6136-6144	VBN	denotes	isolated
T20896	6145-6149	IN	denotes	from
T20897	6150-6161	NNS	denotes	splenocytes
T20898	6162-6167	VBG	denotes	using
T20899	6168-6171	DT	denotes	the
T20900	6172-6178	NNP	denotes	Dneasy
T20901	6179-6185	NNP	denotes	Tissue
T20902	6186-6189	NNP	denotes	Kit
T20903	6190-6191	-LRB-	denotes	(
T20904	6191-6197	NNP	denotes	Qiagen
T20905	6197-6198	-RRB-	denotes	)
T20906	6198-6199	,	denotes	,
T20907	6200-6204	IN	denotes	with
T20908	6205-6210	NNP	denotes	Rnase
T20909	6211-6220	NN	denotes	treatment
T20910	6220-6221	.	denotes	.
T20911	6222-6225	NNP	denotes	PCR
T20912	6226-6235	NNS	denotes	reactions
T20913	6236-6245	VBD	denotes	contained
T20914	6246-6249	CD	denotes	100
T20915	6250-6252	NN	denotes	ng
T20916	6253-6255	IN	denotes	of
T20917	6256-6263	JJ	denotes	genomic
T20918	6264-6267	NNP	denotes	DNA
T20919	6267-6268	,	denotes	,
T20920	6269-6272	CD	denotes	0.2
T20921	6273-6275	NNP	denotes	μM
T20922	6276-6280	DT	denotes	each
T20923	6281-6287	NN	denotes	primer
T20924	6287-6288	,	denotes	,
T20925	6289-6292	CD	denotes	0.2
T20926	6293-6295	NNP	denotes	mM
T20927	6296-6301	NNP	denotes	dNTPs
T20928	6301-6302	,	denotes	,
T20929	6303-6306	CC	denotes	and
T20930	6307-6310	CD	denotes	2.5
T20931	6311-6312	NNP	denotes	U
T20932	6313-6316	NNP	denotes	Taq
T20933	6317-6320	NNP	denotes	DNA
T20934	6321-6331	NN	denotes	polymerase
T20935	6332-6333	-LRB-	denotes	(
T20936	6333-6336	NNP	denotes	NEB
T20937	6336-6337	-RRB-	denotes	)
T20938	6338-6347	VBN	denotes	performed
T20939	6348-6350	IN	denotes	in
T20940	6351-6353	NNP	denotes	1X
T20941	6354-6363	NNP	denotes	ThermoPol
T20942	6364-6370	NN	denotes	buffer
T20943	6371-6372	-LRB-	denotes	(
T20944	6372-6375	NNP	denotes	NEB
T20945	6375-6376	-RRB-	denotes	)
T20946	6376-6377	.	denotes	.
T20947	6378-6385	NNS	denotes	Primers
T20948	6386-6390	VBN	denotes	used
T20949	6391-6395	VBD	denotes	were
T20950	6396-6401	NNP	denotes	Vκcon
T20951	6402-6405	CC	denotes	and
T20952	6406-6409	NNP	denotes	Jκ5
T20953	6410-6411	CD	denotes	1
T20954	6411-6412	CD	denotes	°
T20955	6413-6416	CC	denotes	and
T20956	6417-6421	VBP	denotes	have
T20957	6422-6426	VBN	denotes	been
T20958	6427-6436	VBN	denotes	described
T20959	6437-6447	RB	denotes	previously
T20960	6448-6449	NNP	denotes	[
T20961	6449-6451	CD	denotes	74
T20962	6451-6452	NNP	denotes	]
T20963	6452-6453	.	denotes	.
T20964	6454-6457	NNP	denotes	PCR
T20965	6458-6468	NNS	denotes	conditions
T20966	6469-6473	VBD	denotes	were
T20967	6474-6476	CD	denotes	94
T20968	6477-6478	NN	denotes	°
T20969	6478-6479	NNP	denotes	C
T20970	6480-6483	IN	denotes	for
T20971	6484-6485	CD	denotes	2
T20972	6486-6489	NN	denotes	min
T20973	6489-6490	,	denotes	,
T20974	6491-6493	CD	denotes	40
T20975	6494-6500	NNS	denotes	cycles
T20976	6501-6503	IN	denotes	of
T20977	6504-6506	CD	denotes	94
T20978	6507-6508	CD	denotes	°
T20979	6508-6509	NNP	denotes	C
T20980	6510-6513	IN	denotes	for
T20981	6514-6516	CD	denotes	30
T20982	6517-6518	PRP	denotes	s
T20983	6518-6519	,	denotes	,
T20984	6520-6522	CD	denotes	63
T20985	6523-6524	NN	denotes	°
T20986	6524-6525	NNP	denotes	C
T20987	6526-6529	IN	denotes	for
T20988	6530-6532	CD	denotes	90
T20989	6533-6534	PRP	denotes	s
T20990	6534-6535	,	denotes	,
T20991	6536-6539	CC	denotes	and
T20992	6540-6542	CD	denotes	72
T20993	6543-6544	NN	denotes	°
T20994	6544-6545	NNP	denotes	C
T20995	6546-6549	IN	denotes	for
T20996	6550-6551	CD	denotes	1
T20997	6552-6555	NN	denotes	min
T20998	6555-6556	,	denotes	,
T20999	6557-6565	VBN	denotes	followed
T21000	6566-6568	IN	denotes	by
T21001	6569-6570	CD	denotes	1
T21002	6571-6576	NN	denotes	cycle
T21003	6577-6579	IN	denotes	of
T21004	6580-6582	CD	denotes	72
T21005	6583-6584	CD	denotes	°
T21006	6584-6585	NNP	denotes	C
T21007	6586-6589	IN	denotes	for
T21008	6590-6591	CD	denotes	5
T21009	6592-6595	NN	denotes	min
T21010	6595-6596	.	denotes	.
T21195	6598-6608	NNP	denotes	Immunoblot
T21196	6609-6617	NN	denotes	analysis
T21197	6617-6618	.	denotes	.
T21198	6619-6624	JJ	denotes	Whole
T21199	6625-6629	NN	denotes	cell
T21200	6630-6637	NNS	denotes	lysates
T21201	6638-6642	VBD	denotes	were
T21202	6643-6651	VBN	denotes	prepared
T21203	6652-6654	IN	denotes	in
T21204	6655-6679	NN	denotes	radioimmunoprecipitation
T21205	6680-6685	NN	denotes	assay
T21206	6686-6687	-LRB-	denotes	(
T21207	6687-6691	NNP	denotes	RIPA
T21208	6691-6692	-RRB-	denotes	)
T21209	6693-6699	NN	denotes	buffer
T21210	6700-6701	-LRB-	denotes	(
T21211	6701-6703	CD	denotes	20
T21212	6704-6706	NNP	denotes	mM
T21213	6707-6715	NNP	denotes	Tris-HCl
T21214	6716-6717	NNP	denotes	[
T21215	6717-6719	NNP	denotes	pH
T21216	6720-6723	CD	denotes	7.5
T21217	6723-6724	NNP	denotes	]
T21218	6724-6725	,	denotes	,
T21219	6726-6729	CD	denotes	150
T21220	6730-6732	NNP	denotes	mM
T21221	6733-6737	NNP	denotes	NaCl
T21222	6737-6738	,	denotes	,
T21223	6739-6740	CD	denotes	1
T21224	6741-6743	NNP	denotes	mM
T21225	6744-6748	NNP	denotes	EDTA
T21226	6748-6749	,	denotes	,
T21227	6750-6751	CD	denotes	1
T21228	6751-6752	NN	denotes	%
T21229	6753-6758	NN	denotes	NP-40
T21230	6758-6759	,	denotes	,
T21231	6760-6763	CD	denotes	0.1
T21232	6763-6764	NN	denotes	%
T21233	6765-6768	NNP	denotes	SDS
T21234	6768-6769	,	denotes	,
T21235	6770-6773	CD	denotes	0.1
T21236	6773-6774	NN	denotes	%
T21237	6775-6781	NN	denotes	sodium
T21238	6782-6794	NN	denotes	deoxycholate
T21239	6794-6795	-RRB-	denotes	)
T21240	6796-6808	VBN	denotes	supplemented
T21241	6809-6813	IN	denotes	with
T21242	6814-6815	CD	denotes	2
T21243	6816-6818	NNP	denotes	mM
T21244	6819-6839	NN	denotes	phenylmethylsulfonyl
T21245	6840-6848	NN	denotes	fluoride
T21246	6848-6849	,	denotes	,
T21247	6850-6851	CD	denotes	1
T21248	6852-6854	NNP	denotes	mM
T21249	6855-6861	NNP	denotes	Na3VO4
T21250	6861-6862	,	denotes	,
T21251	6863-6866	CC	denotes	and
T21252	6867-6872	CD	denotes	1:100
T21253	6873-6893	JJ	denotes	protease/phosphatase
T21254	6894-6903	NN	denotes	inhibitor
T21255	6904-6913	NNS	denotes	cocktails
T21256	6914-6915	-LRB-	denotes	(
T21257	6915-6920	NNP	denotes	Sigma
T21258	6920-6921	-RRB-	denotes	)
T21259	6921-6922	.	denotes	.
T21260	6923-6928	JJ	denotes	Crude
T21261	6929-6936	NNS	denotes	lysates
T21262	6937-6941	VBD	denotes	were
T21263	6942-6953	VBN	denotes	centrifuged
T21264	6954-6956	IN	denotes	at
T21265	6957-6963	CD	denotes	13,000
T21266	6964-6967	NN	denotes	rpm
T21267	6968-6971	IN	denotes	for
T21268	6972-6974	CD	denotes	10
T21269	6975-6978	NN	denotes	min
T21270	6979-6981	IN	denotes	at
T21271	6982-6983	CD	denotes	4
T21272	6984-6985	CD	denotes	°
T21273	6985-6986	NNP	denotes	C
T21274	6987-6990	CC	denotes	and
T21275	6991-6994	DT	denotes	the
T21276	6995-7007	NNS	denotes	supernatants
T21277	7008-7012	VBD	denotes	were
T21278	7013-7022	VBN	denotes	collected
T21279	7023-7026	IN	denotes	for
T21280	7027-7034	JJ	denotes	further
T21281	7035-7043	NN	denotes	analysis
T21282	7043-7044	.	denotes	.
T21283	7045-7052	NN	denotes	Protein
T21284	7053-7067	NNS	denotes	concentrations
T21285	7068-7072	VBD	denotes	were
T21286	7073-7083	VBN	denotes	determined
T21287	7084-7088	IN	denotes	with
T21288	7089-7092	DT	denotes	the
T21289	7093-7100	NNP	denotes	Bio-Rad
T21290	7101-7103	NNP	denotes	DC
T21291	7104-7111	NN	denotes	protein
T21292	7112-7117	NN	denotes	assay
T21293	7118-7124	NN	denotes	system
T21294	7124-7125	.	denotes	.
T21295	7126-7133	NNS	denotes	Lysates
T21296	7134-7138	VBD	denotes	were
T21297	7139-7145	VBN	denotes	boiled
T21298	7146-7148	IN	denotes	in
T21299	7149-7152	DT	denotes	the
T21300	7153-7161	NN	denotes	presence
T21301	7162-7164	IN	denotes	of
T21302	7165-7168	CD	denotes	2.5
T21303	7168-7169	NN	denotes	%
T21304	7170-7186	NN	denotes	β-mecaptoethanol
T21305	7186-7187	,	denotes	,
T21306	7188-7197	VBN	denotes	separated
T21307	7198-7200	IN	denotes	by
T21308	7201-7211	VBG	denotes	denaturing
T21309	7212-7220	NNP	denotes	SDS-PAGE
T21310	7220-7221	,	denotes	,
T21311	7222-7225	CC	denotes	and
T21312	7226-7237	VBD	denotes	transferred
T21313	7238-7240	TO	denotes	to
T21314	7241-7245	CD	denotes	0.45
T21315	7245-7246	:	denotes	-
T21316	7246-7248	NN	denotes	μm
T21317	7249-7257	NNP	denotes	Optitran
T21318	7258-7267	NNS	denotes	membranes
T21319	7268-7269	-LRB-	denotes	(
T21320	7269-7279	NNP	denotes	Schleicher
T21321	7280-7281	CC	denotes	&
T21322	7282-7289	NNP	denotes	Schuell
T21323	7289-7290	-RRB-	denotes	)
T21324	7291-7293	IN	denotes	in
T21325	7294-7295	DT	denotes	a
T21326	7296-7303	NNP	denotes	Bio-Rad
T21327	7304-7312	NN	denotes	transfer
T21328	7313-7317	NN	denotes	unit
T21329	7317-7318	.	denotes	.
T21330	7319-7328	NNS	denotes	Membranes
T21331	7329-7333	VBD	denotes	were
T21332	7334-7347	VBN	denotes	immunoblotted
T21333	7348-7352	IN	denotes	with
T21334	7353-7356	DT	denotes	the
T21335	7357-7368	JJ	denotes	appropriate
T21336	7369-7376	JJ	denotes	primary
T21337	7377-7385	NN	denotes	antibody
T21338	7386-7394	VBN	denotes	followed
T21339	7395-7397	IN	denotes	by
T21340	7398-7409	JJ	denotes	horseradish
T21341	7410-7420	NN	denotes	peroxidase
T21342	7421-7427	VBD	denotes	tagged
T21343	7428-7437	JJ	denotes	secondary
T21344	7438-7448	NNS	denotes	antibodies
T21345	7449-7450	-LRB-	denotes	(
T21346	7450-7458	NNP	denotes	Amersham
T21347	7459-7470	NNPS	denotes	Biosciences
T21348	7471-7474	CC	denotes	and
T21349	7475-7479	NNP	denotes	Dako
T21350	7479-7480	-RRB-	denotes	)
T21351	7481-7484	CC	denotes	and
T21352	7485-7493	VBN	denotes	detected
T21353	7494-7498	IN	denotes	with
T21354	7499-7502	DT	denotes	the
T21355	7503-7514	NNP	denotes	SuperSignal
T21356	7515-7519	NNP	denotes	West
T21357	7520-7524	NNP	denotes	Pico
T21358	7525-7531	NNP	denotes	System
T21359	7532-7533	-LRB-	denotes	(
T21360	7533-7539	NNP	denotes	Pierce
T21361	7539-7540	-RRB-	denotes	)
T21362	7540-7541	.	denotes	.
T21474	7543-7553	NNS	denotes	Antibodies
T21475	7553-7554	.	denotes	.
T21476	7555-7570	JJ	denotes	FITC-conjugated
T21477	7571-7575	NN	denotes	goat
T21478	7576-7586	JJ	denotes	anti-mouse
T21479	7587-7590	NNP	denotes	IgM
T21480	7590-7591	,	denotes	,
T21481	7592-7595	NN	denotes	rat
T21482	7596-7602	NNP	denotes	IgG2aκ
T21483	7603-7613	JJ	denotes	anti-mouse
T21484	7614-7617	NNP	denotes	IgD
T21485	7618-7619	-LRB-	denotes	(
T21486	7619-7624	VBP	denotes	clone
T21487	7625-7627	CD	denotes	11
T21488	7628-7630	CD	denotes	26
T21489	7630-7631	-RRB-	denotes	)
T21490	7631-7632	,	denotes	,
T21491	7633-7636	NN	denotes	rat
T21492	7637-7642	NNP	denotes	IgG1κ
T21493	7643-7653	JJ	denotes	anti-mouse
T21494	7654-7659	NN	denotes	kappa
T21495	7660-7661	-LRB-	denotes	(
T21496	7661-7666	JJ	denotes	clone
T21497	7667-7672	CD	denotes	187.1
T21498	7672-7673	-RRB-	denotes	)
T21499	7673-7674	,	denotes	,
T21500	7675-7678	NN	denotes	rat
T21501	7679-7685	NNP	denotes	IgG2bκ
T21502	7686-7696	JJ	denotes	anti-mouse
T21503	7697-7703	NN	denotes	lambda
T21504	7704-7705	-LRB-	denotes	(
T21505	7705-7710	JJ	denotes	clone
T21506	7711-7714	NNS	denotes	JC5
T21507	7715-7716	LS	denotes	1
T21508	7716-7717	-RRB-	denotes	)
T21509	7717-7718	:	denotes	;
T21510	7719-7732	VBN	denotes	PE-conjugated
T21511	7733-7737	NN	denotes	goat
T21512	7738-7748	NN	denotes	anti-mouse
T21513	7749-7752	NNP	denotes	IgG
T21514	7752-7753	:	denotes	;
T21515	7754-7767	JJ	denotes	un-conjugated
T21516	7768-7772	JJ	denotes	goat
T21517	7773-7783	JJ	denotes	anti-mouse
T21518	7784-7789	NN	denotes	kappa
T21519	7790-7793	CC	denotes	and
T21520	7794-7804	JJ	denotes	anti-mouse
T21521	7805-7811	NNS	denotes	lambda
T21522	7812-7816	VBD	denotes	were
T21523	7817-7826	VBN	denotes	purchased
T21524	7827-7831	IN	denotes	from
T21525	7832-7840	NNP	denotes	Southern
T21526	7841-7848	NNP	denotes	Biotech
T21527	7848-7849	.	denotes	.
T21528	7850-7864	JJ	denotes	APC-conjugated
T21529	7865-7868	NN	denotes	rat
T21530	7869-7875	NNP	denotes	IgG2aκ
T21531	7876-7886	JJ	denotes	anti-mouse
T21532	7887-7891	NNP	denotes	CD19
T21533	7892-7893	-LRB-	denotes	(
T21534	7893-7898	VBN	denotes	clone
T21535	7899-7902	NNP	denotes	6D5
T21536	7902-7903	-RRB-	denotes	)
T21537	7903-7904	,	denotes	,
T21538	7905-7918	JJ	denotes	PE-conjugated
T21539	7919-7924	NN	denotes	mouse
T21540	7925-7931	NNP	denotes	IgG2aκ
T21541	7932-7941	NNP	denotes	anti-LMP1
T21542	7942-7943	-LRB-	denotes	(
T21543	7943-7948	VBN	denotes	clone
T21544	7949-7952	NNP	denotes	S12
T21545	7952-7953	-RRB-	denotes	)
T21546	7953-7954	,	denotes	,
T21547	7955-7968	JJ	denotes	un-conjugated
T21548	7969-7974	NN	denotes	mouse
T21549	7975-7980	NNP	denotes	IgG2a
T21550	7981-7988	NNP	denotes	anti-Rb
T21551	7989-7990	-LRB-	denotes	(
T21552	7990-7995	VBN	denotes	clone
T21553	7996-7997	LS	denotes	2
T21554	7997-7998	-RRB-	denotes	)
T21555	7998-7999	,	denotes	,
T21556	8000-8003	CC	denotes	and
T21557	8004-8013	JJ	denotes	anti-Cdk2
T21558	8014-8015	-LRB-	denotes	(
T21559	8015-8020	JJ	denotes	clone
T21560	8021-8023	CD	denotes	55
T21561	8023-8024	-RRB-	denotes	)
T21562	8025-8029	VBD	denotes	were
T21563	8030-8039	VBN	denotes	purchased
T21564	8040-8044	IN	denotes	from
T21565	8045-8047	NNP	denotes	BD
T21566	8048-8058	NNP	denotes	Bioscience
T21567	8058-8059	.	denotes	.
T21568	8060-8073	JJ	denotes	PE-conjugated
T21569	8074-8077	NN	denotes	rat
T21570	8078-8084	NNP	denotes	IgG2aκ
T21571	8085-8095	JJ	denotes	anti-mouse
T21572	8096-8099	NNS	denotes	CD5
T21573	8100-8101	-LRB-	denotes	(
T21574	8101-8106	NN	denotes	clone
T21575	8107-8109	CD	denotes	53
T21576	8110-8113	CD	denotes	7.3
T21577	8113-8114	-RRB-	denotes	)
T21578	8115-8118	VBD	denotes	was
T21579	8119-8128	VBN	denotes	purchased
T21580	8129-8133	IN	denotes	from
T21581	8134-8145	NNP	denotes	eBioscience
T21582	8145-8146	.	denotes	.
T21583	8147-8150	NN	denotes	Rat
T21584	8151-8160	NN	denotes	anti-LMP1
T21585	8161-8162	-LRB-	denotes	(
T21586	8162-8168	NNS	denotes	clones
T21587	8169-8172	NNP	denotes	8G3
T21588	8172-8173	,	denotes	,
T21589	8174-8177	NNP	denotes	1G6
T21590	8177-8178	,	denotes	,
T21591	8179-8183	NNP	denotes	7E10
T21592	8183-8184	,	denotes	,
T21593	8185-8188	CC	denotes	and
T21594	8189-8192	NNP	denotes	7G8
T21595	8192-8193	-RRB-	denotes	)
T21596	8194-8197	VBD	denotes	was
T21597	8198-8207	VBN	denotes	purchased
T21598	8208-8212	IN	denotes	from
T21599	8213-8221	NNP	denotes	Ascenion
T21600	8221-8222	.	denotes	.
T21601	8223-8229	NN	denotes	Rabbit
T21602	8230-8239	JJ	denotes	anti-pAkt
T21603	8240-8241	-LRB-	denotes	(
T21604	8241-8247	JJ	denotes	Ser473
T21605	8247-8248	-RRB-	denotes	)
T21606	8248-8249	,	denotes	,
T21607	8250-8261	JJ	denotes	anti-pGSK3α
T21608	8261-8262	NN	denotes	/
T21609	8262-8263	NN	denotes	β
T21610	8264-8265	-LRB-	denotes	(
T21611	8265-8272	CD	denotes	Ser21/9
T21612	8272-8273	-RRB-	denotes	)
T21613	8273-8274	,	denotes	,
T21614	8275-8286	JJ	denotes	anti-pStat3
T21615	8287-8288	-LRB-	denotes	(
T21616	8288-8294	NNS	denotes	Tyr705
T21617	8294-8295	-RRB-	denotes	)
T21618	8295-8296	,	denotes	,
T21619	8297-8308	JJ	denotes	anti-pStat6
T21620	8309-8310	-LRB-	denotes	(
T21621	8310-8316	NNP	denotes	Tyr641
T21622	8316-8317	-RRB-	denotes	)
T21623	8317-8318	,	denotes	,
T21624	8319-8329	JJ	denotes	anti-pmTOR
T21625	8330-8331	-LRB-	denotes	(
T21626	8331-8338	CD	denotes	Ser2448
T21627	8338-8339	-RRB-	denotes	)
T21628	8339-8340	,	denotes	,
T21629	8341-8351	JJ	denotes	anti-Stat6
T21630	8351-8352	,	denotes	,
T21631	8353-8361	JJ	denotes	anti-Akt
T21632	8361-8362	,	denotes	,
T21633	8363-8373	JJ	denotes	anti-FoxO1
T21634	8373-8374	,	denotes	,
T21635	8375-8378	CC	denotes	and
T21636	8379-8387	JJ	denotes	anti-p27
T21637	8388-8392	VBD	denotes	were
T21638	8393-8402	VBN	denotes	purchased
T21639	8403-8407	IN	denotes	from
T21640	8408-8412	NNP	denotes	Cell
T21641	8413-8422	NNP	denotes	Signaling
T21642	8422-8423	.	denotes	.
T21643	8424-8430	NN	denotes	Rabbit
T21644	8431-8441	NN	denotes	anti-Stat3
T21645	8442-8443	-LRB-	denotes	(
T21646	8443-8448	NNP	denotes	H-190
T21647	8448-8449	-RRB-	denotes	)
T21648	8449-8450	,	denotes	,
T21649	8451-8460	JJ	denotes	anti-IκBα
T21650	8461-8462	-LRB-	denotes	(
T21651	8462-8466	NN	denotes	C-21
T21652	8466-8467	-RRB-	denotes	)
T21653	8467-8468	,	denotes	,
T21654	8469-8472	CC	denotes	and
T21655	8473-8477	NN	denotes	goat
T21656	8478-8484	JJ	denotes	anti-β
T21657	8485-8490	NN	denotes	actin
T21658	8491-8492	-LRB-	denotes	(
T21659	8492-8496	NN	denotes	I-19
T21660	8496-8497	-RRB-	denotes	)
T21661	8498-8502	VBD	denotes	were
T21662	8503-8512	VBN	denotes	purchased
T21663	8513-8517	IN	denotes	from
T21664	8518-8523	NNP	denotes	Santa
T21665	8524-8528	NNP	denotes	Cruz
T21666	8529-8542	NNP	denotes	Biotechnology
T21667	8542-8543	.	denotes	.
T21668	8544-8549	NNP	denotes	Mouse
T21669	8550-8555	NNP	denotes	IgG1κ
T21670	8556-8565	NN	denotes	anti-GSK3
T21671	8566-8569	VBD	denotes	was
T21672	8570-8579	VBN	denotes	purchased
T21673	8580-8584	IN	denotes	from
T21674	8585-8592	NNP	denotes	Upstate
T21675	8593-8606	NNP	denotes	Biotechnology
T21676	8606-8607	.	denotes	.
T21677	8608-8614	NN	denotes	Rabbit
T21678	8615-8623	JJ	denotes	anti-pRb
T21679	8624-8625	-LRB-	denotes	(
T21680	8625-8631	JJ	denotes	Thr373
T21681	8631-8632	-RRB-	denotes	)
T21682	8633-8636	VBD	denotes	was
T21683	8637-8646	VBN	denotes	purchased
T21684	8647-8651	IN	denotes	from
T21685	8652-8655	NNP	denotes	EMD
T21686	8656-8667	NNPS	denotes	Biosciences
T21687	8667-8668	.	denotes	.
R14187	T18431	T18432	compound	Transgenic,mice
R14188	T18432	T18432	ROOT	mice,mice
R14189	T18433	T18432	punct	.,mice
R14190	T18434	T18448	nsubjpass	Generation,described
R14191	T18435	T18434	prep	of,Generation
R14192	T18436	T18437	compound	LMP1,mice
R14193	T18437	T18435	pobj	mice,of
R14194	T18438	T18434	prep	under,Generation
R14195	T18439	T18443	det	the,promoter
R14196	T18440	T18443	nmod	Ig,promoter
R14197	T18441	T18443	amod	heavy,promoter
R14198	T18442	T18443	compound	chain,promoter
R14199	T18443	T18438	pobj	promoter,under
R14200	T18444	T18443	cc	and,promoter
R14201	T18445	T18443	conj	enhancer,promoter
R14202	T18446	T18448	aux	have,described
R14203	T18447	T18448	auxpass	been,described
R14204	T18448	T18448	ROOT	described,described
R14205	T18449	T18448	advmod	previously,described
R14206	T18450	T18448	cc	and,described
R14207	T18451	T18452	auxpass	were,maintained
R14208	T18452	T18448	conj	maintained,described
R14209	T18453	T18452	prep	as,maintained
R14210	T18454	T18453	pobj	heterozygotes,as
R14211	T18455	T18454	prep	on,heterozygotes
R14212	T18456	T18458	det	a,background
R14213	T18457	T18458	compound	Balbc,background
R14214	T18458	T18455	pobj	background,on
R14215	T18459	T18461	nmod	[,]
R14216	T18460	T18461	nummod	26,]
R14217	T18461	T18458	appos	],background
R14218	T18462	T18448	punct	.,described
R14219	T18463	T18464	nummod	LMP1,mice
R14220	T18464	T18466	nsubjpass	mice,genotyped
R14221	T18465	T18466	auxpass	were,genotyped
R14222	T18466	T18466	ROOT	genotyped,genotyped
R14223	T18467	T18466	agent	by,genotyped
R14224	T18468	T18469	compound	Southern,blot
R14225	T18469	T18467	pobj	blot,by
R14226	T18470	T18469	cc	and,blot
R14227	T18471	T18472	compound	PCR,analysis
R14228	T18472	T18469	conj	analysis,blot
R14229	T18473	T18472	prep	of,analysis
R14230	T18474	T18475	compound	tail,DNA
R14231	T18475	T18473	pobj	DNA,of
R14232	T18476	T18477	mark	as,described
R14233	T18477	T18466	advcl	described,genotyped
R14234	T18478	T18481	advmod	previously,]
R14235	T18479	T18481	nmod	[,]
R14236	T18480	T18481	nummod	26,]
R14237	T18481	T18477	dobj	],described
R14238	T18482	T18466	punct	.,genotyped
R14239	T18483	T18488	nsubjpass	Spleen,fixed
R14240	T18484	T18483	cc	and,Spleen
R14241	T18485	T18486	compound	liver,sections
R14242	T18486	T18488	nsubjpass	sections,fixed
R14243	T18487	T18488	auxpass	were,fixed
R14244	T18488	T18488	ROOT	fixed,fixed
R14245	T18489	T18488	prep	in,fixed
R14246	T18490	T18491	nummod	4,%
R14247	T18491	T18492	compound	%,paraformaldehyde
R14248	T18492	T18489	pobj	paraformaldehyde,in
R14249	T18493	T18488	cc	and,fixed
R14250	T18494	T18488	conj	embedded,fixed
R14251	T18495	T18494	prep	in,embedded
R14252	T18496	T18495	pobj	paraffin,in
R14253	T18497	T18494	punct	",",embedded
R14254	T18498	T18494	cc	and,embedded
R14255	T18499	T18500	amod	5-μm,sections
R14256	T18500	T18502	nsubjpass	sections,stained
R14257	T18501	T18502	auxpass	were,stained
R14258	T18502	T18494	conj	stained,embedded
R14259	T18503	T18502	prep	with,stained
R14260	T18504	T18503	pobj	hematoxylin,with
R14261	T18505	T18504	cc	and,hematoxylin
R14262	T18506	T18504	conj	eosin,hematoxylin
R14263	T18507	T18502	prep	for,stained
R14264	T18508	T18509	amod	histopathological,analysis
R14265	T18509	T18507	pobj	analysis,for
R14266	T18510	T18502	punct	.,stained
R14267	T18511	T18513	nsubjpass	Lymphomas,passaged
R14268	T18512	T18513	auxpass	were,passaged
R14269	T18513	T18513	ROOT	passaged,passaged
R14270	T18514	T18513	agent	by,passaged
R14271	T18515	T18516	amod	intraperitoneal,injection
R14272	T18516	T18514	pobj	injection,by
R14273	T18517	T18516	prep	of,injection
R14274	T18518	T18521	nummod	1,splenocytes
R14275	T18519	T18521	nummod	×,splenocytes
R14276	T18520	T18521	nummod	108,splenocytes
R14277	T18521	T18517	pobj	splenocytes,of
R14278	T18522	T18516	prep	into,injection
R14279	T18523	T18524	compound	SCID,mice
R14280	T18524	T18522	pobj	mice,into
R14281	T18525	T18513	cc	and,passaged
R14282	T18526	T18513	conj	sacrificed,passaged
R14283	T18527	T18526	prep	upon,sacrificed
R14284	T18528	T18527	pobj	development,upon
R14285	T18529	T18528	prep	of,development
R14286	T18530	T18532	det	an,abdomen
R14287	T18531	T18532	amod	extended,abdomen
R14288	T18532	T18529	pobj	abdomen,of
R14289	T18533	T18513	punct	.,passaged
R14290	T18534	T18536	nsubjpass	Animals,housed
R14291	T18535	T18536	auxpass	were,housed
R14292	T18536	T18536	ROOT	housed,housed
R14293	T18537	T18536	prep	in,housed
R14294	T18538	T18539	det	the,Association
R14295	T18539	T18537	pobj	Association,in
R14296	T18540	T18539	prep	for,Association
R14297	T18541	T18540	pobj	Assessment,for
R14298	T18542	T18541	cc	and,Assessment
R14299	T18543	T18547	nsubj	Accreditation,approved
R14300	T18544	T18543	prep	for,Accreditation
R14301	T18545	T18546	compound	Animal,Care
R14302	T18546	T18544	pobj	Care,for
R14303	T18547	T18536	advcl	approved,housed
R14304	T18548	T18549	compound	animal,facility
R14305	T18549	T18547	dobj	facility,approved
R14306	T18550	T18547	prep	at,approved
R14307	T18551	T18552	det	the,University
R14308	T18552	T18550	pobj	University,at
R14309	T18553	T18552	prep	of,University
R14310	T18554	T18555	compound	North,Carolina
R14311	T18555	T18553	pobj	Carolina,of
R14312	T18556	T18552	prep	at,University
R14313	T18557	T18558	compound	Chapel,Hill
R14314	T18558	T18556	pobj	Hill,at
R14315	T18559	T18547	punct	.,approved
R14316	T18560	T18561	det	All,protocols
R14317	T18561	T18563	nsubjpass	protocols,approved
R14318	T18562	T18563	auxpass	were,approved
R14319	T18563	T18563	ROOT	approved,approved
R14320	T18564	T18563	agent	by,approved
R14321	T18565	T18571	det	the,Committee
R14322	T18566	T18568	compound	Institutional,Care
R14323	T18567	T18568	compound	Animal,Care
R14324	T18568	T18571	nmod	Care,Committee
R14325	T18569	T18568	cc	and,Care
R14326	T18570	T18571	compound	Use,Committee
R14327	T18571	T18564	pobj	Committee,by
R14328	T18572	T18563	punct	.,approved
R14513	T18800	T18800	ROOT	Isolation,Isolation
R14514	T18801	T18800	cc	and,Isolation
R14515	T18802	T18800	conj	growth,Isolation
R14516	T18803	T18802	prep	of,growth
R14517	T18804	T18805	compound	B,cells
R14518	T18805	T18803	pobj	cells,of
R14519	T18806	T18800	punct	.,Isolation
R14520	T18807	T18809	nsubjpass	Splenocytes,prepared
R14521	T18808	T18809	auxpass	were,prepared
R14522	T18809	T18809	ROOT	prepared,prepared
R14523	T18810	T18809	agent	by,prepared
R14524	T18811	T18810	pcomp	homogenizing,by
R14525	T18812	T18813	compound	spleen,tissue
R14526	T18813	T18811	dobj	tissue,homogenizing
R14527	T18814	T18811	prep	with,homogenizing
R14528	T18815	T18817	nummod	two,slides
R14529	T18816	T18817	amod	frosted,slides
R14530	T18817	T18814	pobj	slides,with
R14531	T18818	T18817	cc	and,slides
R14532	T18819	T18817	conj	debris,slides
R14533	T18820	T18821	auxpass	was,filtered
R14534	T18821	T18809	conj	filtered,prepared
R14535	T18822	T18821	prep	through,filtered
R14536	T18823	T18826	det	a,strainer
R14537	T18824	T18825	amod	100-μm,cell
R14538	T18825	T18826	compound	cell,strainer
R14539	T18826	T18822	pobj	strainer,through
R14540	T18827	T18809	punct	.,prepared
R14541	T18828	T18830	nsubjpass	Erythrocytes,lysed
R14542	T18829	T18830	auxpass	were,lysed
R14543	T18830	T18830	ROOT	lysed,lysed
R14544	T18831	T18830	advcl	using,lysed
R14545	T18832	T18833	nummod	0.8,%
R14546	T18833	T18836	compound	%,solution
R14547	T18834	T18835	compound	ammonium,chloride
R14548	T18835	T18836	compound	chloride,solution
R14549	T18836	T18831	dobj	solution,using
R14550	T18837	T18839	punct	(,Technologies
R14551	T18838	T18839	compound	StemCell,Technologies
R14552	T18839	T18836	appos	Technologies,solution
R14553	T18840	T18836	punct	),solution
R14554	T18841	T18831	prep	for,using
R14555	T18842	T18843	nummod	10,min
R14556	T18843	T18841	pobj	min,for
R14557	T18844	T18843	prep	on,min
R14558	T18845	T18844	pobj	ice,on
R14559	T18846	T18830	cc	and,lysed
R14560	T18847	T18830	conj	washed,lysed
R14561	T18848	T18847	advmod	twice,washed
R14562	T18849	T18847	prep	with,washed
R14563	T18850	T18849	pobj	PBS,with
R14564	T18851	T18830	punct	.,lysed
R14565	T18852	T18853	amod	B,cells
R14566	T18853	T18855	nsubjpass	cells,isolated
R14567	T18854	T18855	auxpass	were,isolated
R14568	T18855	T18855	ROOT	isolated,isolated
R14569	T18856	T18855	advcl	using,isolated
R14570	T18857	T18858	compound	CD19-MACS,beads
R14571	T18858	T18856	dobj	beads,using
R14572	T18859	T18855	prep	according,isolated
R14573	T18860	T18859	prep	to,according
R14574	T18861	T18862	det	the,manufacturer
R14575	T18862	T18864	poss	manufacturer,instructions
R14576	T18863	T18862	case	's,manufacturer
R14577	T18864	T18860	pobj	instructions,to
R14578	T18865	T18867	punct	(,Biotec
R14579	T18866	T18867	compound	Miltenyi,Biotec
R14580	T18867	T18864	appos	Biotec,instructions
R14581	T18868	T18867	punct	),Biotec
R14582	T18869	T18855	cc	and,isolated
R14583	T18870	T18855	conj	grown,isolated
R14584	T18871	T18870	prep	in,grown
R14585	T18872	T18874	poss	Iscove,medium
R14586	T18873	T18872	case	's,Iscove
R14587	T18874	T18871	pobj	medium,in
R14588	T18875	T18874	acl	supplemented,medium
R14589	T18876	T18875	prep	with,supplemented
R14590	T18877	T18882	amod	heat-inactivated,serum
R14591	T18878	T18879	nummod	10,%
R14592	T18879	T18882	nmod	%,serum
R14593	T18880	T18882	amod	fetal,serum
R14594	T18881	T18882	amod	bovine,serum
R14595	T18882	T18876	pobj	serum,with
R14596	T18883	T18882	cc	and,serum
R14597	T18884	T18882	conj	antibiotic/antimycotic,serum
R14598	T18885	T18884	punct	(,antibiotic/antimycotic
R14599	T18886	T18884	appos	GIBCO,antibiotic/antimycotic
R14600	T18887	T18884	punct	),antibiotic/antimycotic
R14601	T18888	T18855	punct	.,isolated
R14602	T18889	T18890	nsubj	Splenocytes,isolated
R14603	T18890	T18890	ROOT	isolated,isolated
R14604	T18891	T18890	prep	from,isolated
R14605	T18892	T18893	amod	SCID-passaged,lymphomas
R14606	T18893	T18891	pobj	lymphomas,from
R14607	T18894	T18890	dep	consisted,isolated
R14608	T18895	T18894	prep	of,consisted
R14609	T18896	T18897	nummod	80,%
R14610	T18897	T18895	pobj	%,of
R14611	T18898	T18899	nummod	90,%
R14612	T18899	T18901	compound	%,cells
R14613	T18900	T18901	compound	B,cells
R14614	T18901	T18890	dobj	cells,isolated
R14615	T18902	T18903	mark	as,determined
R14616	T18903	T18890	advcl	determined,isolated
R14617	T18904	T18903	agent	by,determined
R14618	T18905	T18906	compound	flow,cytometry
R14619	T18906	T18904	pobj	cytometry,by
R14620	T18907	T18890	punct	",",isolated
R14621	T18908	T18890	cc	and,isolated
R14622	T18909	T18890	conj	were,isolated
R14623	T18910	T18909	advmod	hence,were
R14624	T18911	T18912	neg	not,further
R14625	T18912	T18913	advmod	further,purified
R14626	T18913	T18909	acomp	purified,were
R14627	T18914	T18913	prep	with,purified
R14628	T18915	T18916	compound	CD19-MACS,beads
R14629	T18916	T18914	pobj	beads,with
R14630	T18917	T18890	punct	.,isolated
R14631	T18918	T18920	nsubjpass	Splenocytes,seeded
R14632	T18919	T18920	auxpass	were,seeded
R14633	T18920	T18920	ROOT	seeded,seeded
R14634	T18921	T18920	prep	at,seeded
R14635	T18922	T18925	nummod	1.25,cells/ml
R14636	T18923	T18925	nummod	×,cells/ml
R14637	T18924	T18925	nummod	106,cells/ml
R14638	T18925	T18921	pobj	cells/ml,at
R14639	T18926	T18925	cc	and,cells/ml
R14640	T18927	T18934	advmod	where,added
R14641	T18928	T18934	advmod	applicable,added
R14642	T18929	T18934	punct	",",added
R14643	T18930	T18931	amod	recombinant,mouse
R14644	T18931	T18932	compound	mouse,IL4
R14645	T18932	T18934	nsubjpass	IL4,added
R14646	T18933	T18934	auxpass	was,added
R14647	T18934	T18920	advcl	added,seeded
R14648	T18935	T18934	prep	at,added
R14649	T18936	T18937	nummod	100,ng/ml
R14650	T18937	T18935	pobj	ng/ml,at
R14651	T18938	T18937	punct	",",ng/ml
R14652	T18939	T18940	compound	recombinant,mouse
R14653	T18940	T18941	compound	mouse,IL10
R14654	T18941	T18937	appos	IL10,ng/ml
R14655	T18942	T18941	prep	at,IL10
R14656	T18943	T18944	nummod	10,ng/ml
R14657	T18944	T18942	pobj	ng/ml,at
R14658	T18945	T18941	punct	",",IL10
R14659	T18946	T18937	cc	and,ng/ml
R14660	T18947	T18950	compound	rat,IL10
R14661	T18948	T18950	compound	IgG1,IL10
R14662	T18949	T18950	compound	anti-mouse,IL10
R14663	T18950	T18937	conj	IL10,ng/ml
R14664	T18951	T18950	cc	and,IL10
R14665	T18952	T18955	nmod	rat,control
R14666	T18953	T18955	amod	IgG1,control
R14667	T18954	T18955	compound	isotype,control
R14668	T18955	T18950	conj	control,IL10
R14669	T18956	T18955	prep	at,control
R14670	T18957	T18958	nummod	10,μg
R14671	T18958	T18960	compound	μg,ml
R14672	T18959	T18960	compound	/,ml
R14673	T18960	T18956	pobj	ml,at
R14674	T18961	T18963	punct	(,Systems
R14675	T18962	T18963	compound	R&D,Systems
R14676	T18963	T18960	appos	Systems,ml
R14677	T18964	T18963	punct	),Systems
R14678	T18965	T18920	punct	.,seeded
R14679	T18966	T18973	prep	For,pulsed
R14680	T18967	T18969	compound	BrdU,assays
R14681	T18968	T18969	compound	incorporation,assays
R14682	T18969	T18966	pobj	assays,For
R14683	T18970	T18973	punct	",",pulsed
R14684	T18971	T18973	nsubjpass	splenocytes,pulsed
R14685	T18972	T18973	auxpass	were,pulsed
R14686	T18973	T18973	ROOT	pulsed,pulsed
R14687	T18974	T18973	prep	for,pulsed
R14688	T18975	T18976	nummod	24,h
R14689	T18976	T18974	pobj	h,for
R14690	T18977	T18976	prep	with,h
R14691	T18978	T18983	nummod	10,post-harvest
R14692	T18979	T18980	compound	μM,BrdU
R14693	T18980	T18983	nmod	BrdU,post-harvest
R14694	T18981	T18980	nummod	1,BrdU
R14695	T18982	T18983	meta	d,post-harvest
R14696	T18983	T18977	pobj	post-harvest,with
R14697	T18984	T18973	punct	.,pulsed
R14965	T19315	T19316	compound	MTS,assay
R14966	T19316	T19316	ROOT	assay,assay
R14967	T19317	T19316	punct	.,assay
R14968	T19318	T19347	det	The,performed
R14969	T19319	T19347	meta	3,performed
R14970	T19320	T19322	punct	-,4
R14971	T19321	T19322	punct	(,4
R14972	T19322	T19319	appos	4,3
R15004	T19354	T19355	compound	cell,proliferation
R15005	T19355	T19356	compound	proliferation,assay
R15006	T19356	T19348	dobj	assay,using
R15007	T19357	T19356	punct	(,assay
R15008	T19358	T19356	appos	Promega,assay
R15009	T19359	T19356	punct	),assay
R15010	T19360	T19347	punct	",",performed
R15011	T19361	T19347	prep	according,performed
R15012	T19362	T19361	prep	to,according
R15013	T19363	T19365	poss	manufacturer,instructions
R15014	T19364	T19363	case	's,manufacturer
R15015	T19365	T19362	pobj	instructions,to
R15016	T19366	T19347	punct	.,performed
R15017	T19367	T19373	prep	For,cultured
R15018	T19368	T19369	nummod	IL4,studies
R15019	T19369	T19367	pobj	studies,For
R15020	T19370	T19373	punct	",",cultured
R15021	T19371	T19373	nsubjpass	splenocytes,cultured
R15022	T19372	T19373	auxpass	were,cultured
R15023	T19373	T19373	ROOT	cultured,cultured
R15024	T19374	T19373	prep	for,cultured
R15025	T19375	T19376	nummod	3,d
R15026	T19376	T19374	pobj	d,for
R15027	T19377	T19373	prep	in,cultured
R15028	T19378	T19379	det	the,presence
R15029	T19379	T19377	pobj	presence,in
R15030	T19380	T19379	cc	or,presence
R15031	T19381	T19379	conj	absence,presence
R15032	T19382	T19381	prep	of,absence
R15033	T19383	T19385	nummod	100,IL4
R15034	T19384	T19385	amod	ng/ml,IL4
R15035	T19385	T19382	pobj	IL4,of
R15036	T19386	T19373	punct	.,cultured
R15037	T19387	T19389	nsubjpass	Cells,seeded
R15038	T19388	T19389	auxpass	were,seeded
R15039	T19389	T19389	ROOT	seeded,seeded
R15040	T19390	T19389	prep	on,seeded
R15041	T19391	T19390	pobj	day,on
R15042	T19392	T19391	nummod	3,day
R15043	T19393	T19389	prep	in,seeded
R15044	T19394	T19393	pobj	triplicate,in
R15045	T19395	T19389	prep	in,seeded
R15046	T19396	T19398	det	a,plate
R15047	T19397	T19398	amod	96-well,plate
R15048	T19398	T19395	pobj	plate,in
R15049	T19399	T19389	prep	at,seeded
R15050	T19400	T19403	nummod	2.5,cells/ml
R15051	T19401	T19403	nmod	×,cells/ml
R15052	T19402	T19403	nummod	106,cells/ml
R15053	T19403	T19399	pobj	cells/ml,at
R15054	T19404	T19389	prep	at,seeded
R15055	T19405	T19406	nummod	100,μl
R15056	T19406	T19404	pobj	μl,at
R15057	T19407	T19408	advmod	per,well
R15058	T19408	T19389	advmod	well,seeded
R15059	T19409	T19389	punct	.,seeded
R15060	T19410	T19411	compound	MTS,reagent
R15061	T19411	T19413	nsubjpass	reagent,added
R15062	T19412	T19413	auxpass	was,added
R15063	T19413	T19428	ccomp	added,subtracted
R15064	T19414	T19413	prep	for,added
R15065	T19415	T19416	nummod	4,h
R15066	T19416	T19414	pobj	h,for
R15067	T19417	T19416	cc	and,h
R15068	T19418	T19416	conj	absorbance,h
R15069	T19419	T19420	auxpass	was,read
R15070	T19420	T19413	conj	read,added
R15071	T19421	T19420	prep	at,read
R15072	T19422	T19423	nummod	540,nm
R15073	T19423	T19421	pobj	nm,at
R15074	T19424	T19428	punct	;,subtracted
R15075	T19425	T19428	nsubjpass	values,subtracted
R15076	T19426	T19425	acl	plotted,values
R15077	T19427	T19428	auxpass	were,subtracted
R15078	T19428	T19428	ROOT	subtracted,subtracted
R15079	T19429	T19428	prep	from,subtracted
R15080	T19430	T19429	pobj	blanks,from
R15081	T19431	T19428	punct	.,subtracted
R15082	T19432	T19438	prep	For,seeded
R15083	T19433	T19434	compound	neutralization,assays
R15084	T19434	T19432	pobj	assays,For
R15085	T19435	T19438	punct	",",seeded
R15086	T19436	T19438	nsubjpass	splenocytes,seeded
R15087	T19437	T19438	auxpass	were,seeded
R15088	T19438	T19438	ROOT	seeded,seeded
R15089	T19439	T19438	prep	at,seeded
R15090	T19440	T19441	nummod	5,×
R15091	T19441	T19439	pobj	×,at
R15092	T19442	T19443	nummod	106,cells/ml
R15093	T19443	T19441	appos	cells/ml,×
R15094	T19444	T19438	prep	at,seeded
R15095	T19445	T19446	nummod	100,μl
R15096	T19446	T19444	pobj	μl,at
R15097	T19447	T19448	advmod	per,well
R15098	T19448	T19438	advmod	well,seeded
R15099	T19449	T19438	prep	on,seeded
R15148	T19498	T19499	auxpass	were,seeded
R15149	T19499	T19499	ROOT	seeded,seeded
R15150	T19500	T19499	prep	at,seeded
R15151	T19501	T19518	nummod	1,added
R15152	T19502	T19518	nsubjpass	×,added
R15153	T19503	T19504	nummod	107,cells/ml
R15154	T19504	T19502	appos	cells/ml,×
R15155	T19505	T19504	prep	at,cells/ml
R15156	T19506	T19507	nummod	100,μl
R15157	T19507	T19505	pobj	μl,at
R15158	T19508	T19509	advmod	per,well
R15159	T19509	T19510	advmod	well,on
R15160	T19510	T19504	prep	on,cells/ml
R15161	T19511	T19510	pobj	day,on
R15162	T19512	T19511	prep	of,day
R15163	T19513	T19512	pobj	harvest,of
R15164	T19514	T19504	punct	",",cells/ml
R15165	T19515	T19504	cc	and,cells/ml
R15166	T19516	T19504	conj	inhibitors,cells/ml
R15167	T19517	T19518	auxpass	were,added
R15168	T19518	T19499	conj	added,seeded
R15169	T19519	T19518	prep	at,added
R15170	T19520	T19521	det	the,concentrations
R15171	T19521	T19519	pobj	concentrations,at
R15172	T19522	T19521	acl	indicated,concentrations
R15173	T19523	T19522	prep	in,indicated
R15174	T19524	T19525	det	the,figures
R15175	T19525	T19523	pobj	figures,in
R15176	T19526	T19518	punct	.,added
R15177	T19527	T19528	det	The,inhibitors
R15178	T19528	T19528	ROOT	inhibitors,inhibitors
R15179	T19529	T19528	appos	BAY11,inhibitors
R15180	T19530	T19529	appos	7085,BAY11
R15181	T19531	T19529	punct	",",BAY11
R15182	T19532	T19529	appos	rapamycin,BAY11
R15183	T19533	T19532	punct	",",rapamycin
R15184	T19534	T19532	conj	triciribine,rapamycin
R15185	T19535	T19534	punct	",",triciribine
R15186	T19536	T19534	conj	U0126,triciribine
R15187	T19537	T19536	punct	",",U0126
R15188	T19538	T19536	conj	SB202190,U0126
R15189	T19539	T19538	punct	",",SB202190
R15190	T19540	T19538	cc	and,SB202190
R15191	T19541	T19538	conj	cucurbitacin,SB202190
R15192	T19542	T19544	nsubjpass	I,purchased
R15193	T19543	T19544	auxpass	were,purchased
R15194	T19544	T19528	relcl	purchased,inhibitors
R15195	T19545	T19544	prep	from,purchased
R15196	T19546	T19547	compound	EMD,Biosciences
R15197	T19547	T19545	pobj	Biosciences,from
R15198	T19548	T19544	punct	.,purchased
R15199	T19549	T19558	prep	For,induced
R15200	T19550	T19552	amod	non-malignant,cultures
R15201	T19551	T19552	compound	splenocyte,cultures
R15202	T19552	T19549	pobj	cultures,For
R15203	T19553	T19558	punct	",",induced
R15204	T19554	T19556	compound	B,activation
R15205	T19555	T19556	compound	cell,activation
R15206	T19556	T19558	nsubjpass	activation,induced
R15208	T19558	T19558	ROOT	induced,induced
R15209	T19559	T19558	prep	with,induced
R15210	T19560	T19561	nummod	10,μg
R15211	T19561	T19563	compound	μg,ml
R15212	T19562	T19563	compound	/,ml
R15213	T19563	T19559	pobj	ml,with
R15214	T19564	T19563	prep	of,ml
R15215	T19565	T19566	compound	goat,F
R15216	T19566	T19564	pobj	F,of
R15217	T19567	T19572	punct	(,IgM
R15218	T19568	T19572	nmod	ab,IgM
R15219	T19569	T19568	case	',ab
R15220	T19570	T19572	punct	),IgM
R15221	T19571	T19572	compound	anti-mouse,IgM
R15222	T19572	T19563	appos	IgM,ml
R15223	T19573	T19575	punct	(,ImmunoResearch
R15224	T19574	T19575	compound	Jackson,ImmunoResearch
R15225	T19575	T19572	appos	ImmunoResearch,IgM
R15226	T19576	T19575	punct	),ImmunoResearch
R15227	T19577	T19558	punct	.,induced
R15228	T19578	T19580	nsubjpass	Cultures,pulsed
R15229	T19579	T19580	auxpass	were,pulsed
R15230	T19580	T19580	ROOT	pulsed,pulsed
R15231	T19581	T19580	prep	for,pulsed
R15232	T19582	T19583	nummod	4,h
R15233	T19583	T19581	pobj	h,for
R15234	T19584	T19583	prep	with,h
R15235	T19585	T19586	compound	MTS,reagent
R15236	T19586	T19584	pobj	reagent,with
R15237	T19587	T19586	nummod	1,reagent
R15238	T19588	T19589	meta	d,post-seeding
R15239	T19589	T19586	appos	post-seeding,reagent
R15240	T19590	T19580	punct	.,pulsed
R15411	T19824	T19824	ROOT	Immunohistochemistry,Immunohistochemistry
R15412	T19825	T19824	punct	.,Immunohistochemistry
R15413	T19826	T19828	amod	Paraffin-embedded,sections
R15414	T19827	T19828	compound	spleen,sections
R15415	T19828	T19830	nsubjpass	sections,deparaffinized
R15416	T19829	T19830	auxpass	were,deparaffinized
R15417	T19830	T19830	ROOT	deparaffinized,deparaffinized
R15418	T19831	T19830	prep	in,deparaffinized
R15419	T19832	T19831	pobj	Histoclear,in
R15420	T19833	T19835	punct	(,Diagnostics
R15421	T19834	T19835	compound	National,Diagnostics
R15422	T19835	T19832	appos	Diagnostics,Histoclear
R15423	T19836	T19835	punct	),Diagnostics
R15424	T19837	T19830	cc	and,deparaffinized
R15425	T19838	T19830	conj	rehydrated,deparaffinized
R15426	T19839	T19838	prep	in,rehydrated
R15427	T19840	T19841	amod	graded,ethanol
R15428	T19841	T19839	pobj	ethanol,in
R15429	T19842	T19830	punct	.,deparaffinized
R15430	T19843	T19844	nsubj	Sections,were
R15431	T19844	T19844	ROOT	were,were
R15432	T19845	T19844	attr	antigen,were
R15433	T19846	T19845	acl	retrieved,antigen
R15434	T19847	T19846	agent	by,retrieved
R15435	T19848	T19847	pobj	microwaving,by
R15436	T19849	T19848	prep	in,microwaving
R15437	T19850	T19851	compound	citrate,buffer
R15438	T19851	T19849	pobj	buffer,in
R15439	T19852	T19853	punct	(,pH
R15440	T19853	T19848	appos	pH,microwaving
R15441	T19854	T19853	nummod	6.0,pH
R15442	T19855	T19853	punct	),pH
R15443	T19856	T19846	prep	for,retrieved
R15444	T19857	T19858	nummod	15,min
R15445	T19858	T19856	pobj	min,for
R15446	T19859	T19858	punct	(,min
R15447	T19860	T19865	nummod	LMP1,min
R15448	T19861	T19860	advmod	staining,LMP1
R15449	T19862	T19860	punct	),LMP1
R15450	T19863	T19860	cc	and,LMP1
R15451	T19864	T19860	conj	10,LMP1
R15452	T19865	T19858	appos	min,min
R15453	T19866	T19868	punct	(,staining
R15454	T19867	T19868	amod	pStat3,staining
R15455	T19868	T19865	appos	staining,min
R15456	T19869	T19865	punct	),min
R15457	T19870	T19844	punct	.,were
R15458	T19871	T19877	prep	For,blocked
R15459	T19872	T19873	nsubj	LMP1,staining
R15460	T19873	T19871	pobj	staining,For
R15461	T19874	T19877	punct	",",blocked
R15462	T19875	T19877	nsubjpass	sections,blocked
R15463	T19876	T19877	auxpass	were,blocked
R15464	T19877	T19877	ROOT	blocked,blocked
R15465	T19878	T19877	prep	with,blocked
R15466	T19879	T19880	nummod	1,%
R15467	T19880	T19881	compound	%,BSA
R15468	T19881	T19878	pobj	BSA,with
R15469	T19882	T19877	cc	and,blocked
R15470	T19883	T19884	nummod	0.1,%
R15471	T19884	T19877	conj	%,blocked
R15472	T19885	T19886	amod	cold,fish
R15473	T19886	T19888	compound	fish,gelatin
R15474	T19887	T19888	compound	skin,gelatin
R15475	T19888	T19889	nsubj	gelatin,followed
R15476	T19889	T19884	relcl	followed,%
R15477	T19890	T19889	prep	with,followed
R15478	T19891	T19892	compound	streptavidin/biotin,block
R15479	T19892	T19890	pobj	block,with
R15480	T19893	T19895	punct	(,Labs
R15481	T19894	T19895	compound	Vector,Labs
R15482	T19895	T19892	appos	Labs,block
R15483	T19896	T19895	punct	),Labs
R15484	T19897	T19889	punct	.,followed
R15485	T19898	T19900	compound	Rat,anti-LMP1
R15486	T19899	T19900	compound	IgG,anti-LMP1
R15487	T19900	T19910	nsubjpass	anti-LMP1,used
R15488	T19901	T19910	punct	(,used
R15489	T19902	T19910	nsubjpass	clones,used
R15490	T19903	T19902	dobj	8G3,clones
R15491	T19904	T19903	cc	and,8G3
R15492	T19905	T19903	conj	1G6,8G3
R15493	T19906	T19905	punct	",",1G6
R15494	T19907	T19905	conj	Ascenion,1G6
R15495	T19908	T19902	punct	),clones
R15496	T19909	T19910	auxpass	were,used
R15497	T19910	T19919	ccomp	used,followed
R15498	T19911	T19910	prep	at,used
R15499	T19912	T19913	nummod	1:10,dilution
R15500	T19913	T19911	pobj	dilution,at
R15501	T19914	T19913	prep	from,dilution
R15502	T19915	T19916	compound	tissue,culture
R15503	T19916	T19917	compound	culture,supernatants
R15504	T19917	T19914	pobj	supernatants,from
R15505	T19918	T19919	punct	",",followed
R15506	T19919	T19919	ROOT	followed,followed
R15507	T19920	T19919	prep	with,followed
R15508	T19921	T19922	nummod	8,μg
R15509	T19922	T19940	nmod	μg,serum
R15510	T19923	T19924	compound	/,ml
R15511	T19924	T19933	nmod	ml,IgG
R15512	T19925	T19927	amod	biotinylated,F
R15513	T19926	T19927	compound	mouse,F
R15514	T19927	T19924	appos	F,ml
R15515	T19928	T19929	punct	(,ab
R15516	T19929	T19927	appos	ab,F
R15517	T19930	T19929	punct	',ab
R15518	T19931	T19924	punct	),ml
R15519	T19932	T19933	amod	anti-rat,IgG
R15520	T19933	T19922	appos	IgG,μg
R15530	T19943	T19940	appos	ImmunoResearch,serum
R15531	T19944	T19940	punct	),serum
R15532	T19945	T19940	cc	and,serum
R15533	T19946	T19947	nummod	2,μg
R15534	T19947	T19940	conj	μg,serum
R15535	T19948	T19952	nmod	/,conjugate
R15536	T19949	T19952	nmod	ml,conjugate
R15537	T19950	T19952	nmod	streptavidin-alkaline,conjugate
R15538	T19951	T19952	compound	phosphatase,conjugate
R15539	T19952	T19947	appos	conjugate,μg
R15540	T19953	T19955	punct	(,ImmunoResearch
R15541	T19954	T19955	compound	Jackson,ImmunoResearch
R15542	T19955	T19952	appos	ImmunoResearch,conjugate
R15543	T19956	T19955	punct	),ImmunoResearch
R15544	T19957	T19919	punct	.,followed
R15545	T19958	T19960	nsubjpass	Stains,developed
R15546	T19959	T19960	auxpass	were,developed
R15547	T19960	T19960	ROOT	developed,developed
R15548	T19961	T19960	prep	with,developed
R15549	T19962	T19961	pobj	BCIP/NBT,with
R15550	T19963	T19960	cc	and,developed
R15551	T19964	T19960	conj	counterstained,developed
R15552	T19965	T19964	prep	in,counterstained
R15553	T19966	T19968	compound	Nuclear,Red
R15554	T19967	T19968	compound	Fast,Red
R15555	T19968	T19965	pobj	Red,in
R15556	T19969	T19968	punct	(,Red
R15557	T19970	T19968	appos	Dako,Red
R15558	T19971	T19968	punct	),Red
R15559	T19972	T19960	punct	.,developed
R15560	T19973	T19975	nsubjpass	Phospho-Stat3,detected
R15561	T19974	T19975	auxpass	was,detected
R15562	T19975	T19975	ROOT	detected,detected
R15563	T19976	T19975	prep	with,detected
R15564	T19977	T19978	nummod	4,μg
R15565	T19978	T19980	compound	μg,ml
R15566	T19979	T19980	compound	/,ml
R15567	T19980	T19976	pobj	ml,with
R15568	T19981	T19980	prep	of,ml
R15569	T19982	T19983	amod	pStat3,antibody
R15570	T19983	T19981	pobj	antibody,of
R15571	T19984	T19988	punct	(,Signaling
R15572	T19985	T19988	npadvmod	Tyr705,Signaling
R15573	T19986	T19988	punct	",",Signaling
R15574	T19987	T19988	compound	Cell,Signaling
R15575	T19988	T19980	appos	Signaling,ml
R15576	T19989	T19988	punct	),Signaling
R15577	T19990	T19975	cc	and,detected
R15578	T19991	T19975	conj	detected,detected
R15579	T19992	T19991	prep	with,detected
R15580	T19993	T19997	amod	anti-rabbit,kit
R15581	T19994	T19995	compound	Poly-HRP,IHC
R15582	T19995	T19997	compound	IHC,kit
R15583	T19996	T19997	compound	detection,kit
R15584	T19997	T19992	pobj	kit,with
R15585	T19998	T19997	punct	(,kit
R15586	T19999	T19997	appos	Chemicon,kit
R15587	T20000	T19997	punct	),kit
R15588	T20001	T19975	punct	.,detected
R15716	T20169	T20170	compound	Flow,cytometry
R15717	T20170	T20170	ROOT	cytometry,cytometry
R15718	T20171	T20170	punct	.,cytometry
R15719	T20172	T20173	compound	One,million
R15720	T20173	T20174	nummod	million,splenocytes
R15721	T20174	T20176	nsubjpass	splenocytes,stained
R15722	T20175	T20176	auxpass	were,stained
R15723	T20176	T20176	ROOT	stained,stained
R15724	T20177	T20176	prep	with,stained
R15725	T20178	T20182	det	the,unconjugated
R15726	T20179	T20182	amod	appropriate,unconjugated
R15727	T20180	T20182	amod	primary,unconjugated
R15728	T20181	T20182	compound	antibody,unconjugated
R15729	T20182	T20177	pobj	unconjugated,with
R15730	T20183	T20182	cc	or,unconjugated
R15731	T20184	T20182	conj	conjugated,unconjugated
R15732	T20185	T20184	prep	to,conjugated
R15733	T20186	T20185	pobj	FITC,to
R15734	T20187	T20186	punct	",",FITC
R15735	T20188	T20186	conj	PE,FITC
R15736	T20189	T20188	punct	",",PE
R15737	T20190	T20188	cc	or,PE
R15738	T20191	T20188	conj	APC,PE
R15739	T20192	T20184	conj	diluted,conjugated
R15740	T20193	T20192	prep	in,diluted
R15741	T20194	T20195	compound	stain,buffer
R15742	T20195	T20193	pobj	buffer,in
R15743	T20196	T20197	punct	(,PBS
R15744	T20197	T20195	amod	PBS,buffer
R15745	T20198	T20192	prep	with,diluted
R15746	T20199	T20200	nummod	3,%
R15747	T20200	T20201	compound	%,FBS
R15748	T20201	T20198	pobj	FBS,with
R15749	T20202	T20176	punct	),stained
R15750	T20203	T20176	punct	.,stained
R15751	T20204	T20213	prep	For,used
R15752	T20205	T20206	compound	BrdU,detection
R15753	T20206	T20204	pobj	detection,For
R15754	T20207	T20213	punct	",",used
R15755	T20208	T20211	det	the,kit
R15756	T20209	T20211	compound	FITC-BrdU,kit
R15757	T20210	T20211	compound	flow,kit
R15784	T20237	T20236	prep	of,exclusion
R15785	T20238	T20239	amod	dead,cells
R15786	T20239	T20237	pobj	cells,of
R15787	T20240	T20236	punct	",",exclusion
R15788	T20241	T20228	conj	stained,exposed
R15789	T20242	T20241	prep	for,stained
R15790	T20243	T20244	compound	surface,antigens
R15791	T20244	T20242	pobj	antigens,for
R15792	T20245	T20244	punct	",",antigens
R15793	T20246	T20244	acl	fixed,antigens
R15794	T20247	T20246	prep	in,fixed
R15795	T20248	T20247	pobj	paraformaldehyde,in
R15796	T20249	T20228	punct	",",exposed
R15797	T20250	T20228	cc	and,exposed
R15798	T20251	T20228	conj	permeabilized,exposed
R15799	T20252	T20251	prep	with,permeabilized
R15800	T20253	T20252	pobj	saponin,with
R15801	T20254	T20228	punct	.,exposed
R15802	T20255	T20256	aux	To,expose
R15803	T20256	T20262	advcl	expose,treated
R15804	T20257	T20258	compound	BrdU,epitopes
R15805	T20258	T20256	dobj	epitopes,expose
R15806	T20259	T20262	punct	",",treated
R15807	T20260	T20262	nsubjpass	cells,treated
R15808	T20261	T20262	auxpass	were,treated
R15809	T20262	T20262	ROOT	treated,treated
R15810	T20263	T20262	prep	with,treated
R15811	T20264	T20263	pobj	Dnase,with
R15815	T20268	T20270	amod	FITC-conjugated,antibody
R15816	T20269	T20270	amod	anti-BrdU,antibody
R15817	T20270	T20267	pobj	antibody,with
R15842	T20295	T20296	nummod	.2,program
R15843	T20296	T20291	pobj	program,on
R15844	T20297	T20296	punct	(,program
R15845	T20298	T20296	appos	Dako,program
R15846	T20299	T20296	punct	),program
R15847	T20300	T20290	punct	.,conducted
R16046	T20534	T20536	amod	Rnase,assay
R16047	T20535	T20536	compound	protection,assay
R16048	T20536	T20536	ROOT	assay,assay
R16049	T20537	T20536	punct	.,assay
R16050	T20538	T20539	amod	Total,RNA
R16051	T20539	T20541	nsubjpass	RNA,isolated
R16052	T20540	T20541	auxpass	was,isolated
R16053	T20541	T20541	ROOT	isolated,isolated
R16054	T20542	T20541	prep	from,isolated
R16055	T20543	T20547	nummod	CD19,cells
R16056	T20544	T20547	nummod	+,cells
R16057	T20545	T20547	amod	MACS-purified,cells
R16058	T20546	T20547	compound	B,cells
R16059	T20547	T20542	pobj	cells,from
R16060	T20548	T20541	advcl	using,isolated
R16061	T20549	T20553	det	the,kit
R16062	T20550	T20553	nmod	Rneasy,kit
R16063	T20551	T20553	amod	midi,kit
R16064	T20552	T20553	compound	purification,kit
R16065	T20553	T20548	dobj	kit,using
R16066	T20554	T20553	prep	with,kit
R16067	T20555	T20556	compound	Dnase,treatment
R16068	T20556	T20554	pobj	treatment,with
R16069	T20557	T20541	punct	",",isolated
R16070	T20558	T20541	prep	according,isolated
R16071	T20559	T20558	prep	to,according
R16072	T20560	T20561	det	the,manufacturer
R16073	T20561	T20563	poss	manufacturer,instructions
R16074	T20562	T20561	case	's,manufacturer
R16075	T20563	T20559	pobj	instructions,to
R16076	T20564	T20565	punct	(,Qiagen
R16077	T20565	T20563	appos	Qiagen,instructions
R16078	T20566	T20563	punct	),instructions
R16079	T20567	T20541	punct	.,isolated
R16080	T20568	T20571	det	The,template
R16081	T20569	T20571	amod	mCK-1,template
R16082	T20570	T20571	compound	probe,template
R16083	T20571	T20578	nsubjpass	template,labeled
R16084	T20572	T20571	acl	set,template
R16085	T20573	T20575	punct	(,Biosciences
R16086	T20574	T20575	compound	BD,Biosciences
R16087	T20575	T20572	appos	Biosciences,set
R16088	T20576	T20572	punct	),set
R16089	T20577	T20578	auxpass	was,labeled
R16090	T20578	T20578	ROOT	labeled,labeled
R16091	T20579	T20578	advcl	using,labeled
R16092	T20580	T20584	det	the,Kit
R16093	T20581	T20584	compound	In,Kit
R16094	T20582	T20583	compound	Vitro,Transcription
R16095	T20583	T20584	compound	Transcription,Kit
R16096	T20584	T20579	dobj	Kit,using
R16097	T20585	T20578	prep	according,labeled
R16098	T20586	T20585	prep	to,according
R16099	T20587	T20588	det	the,manufacturer
R16100	T20588	T20590	poss	manufacturer,instructions
R16101	T20589	T20588	case	's,manufacturer
R16102	T20590	T20586	pobj	instructions,to
R16103	T20591	T20593	punct	(,Biosciences
R16104	T20592	T20593	compound	BD,Biosciences
R16105	T20593	T20590	appos	Biosciences,instructions
R16106	T20594	T20590	punct	),instructions
R16107	T20595	T20578	punct	.,labeled
R16108	T20596	T20605	advmod	Briefly,labeled
R16109	T20597	T20605	punct	",",labeled
R16110	T20598	T20599	nummod	50,ng
R16111	T20599	T20605	nsubjpass	ng,labeled
R16112	T20600	T20599	prep	of,ng
R16113	T20601	T20603	amod	mCK-1,set
R16114	T20602	T20603	compound	probe,set
R16115	T20603	T20600	pobj	set,of
R16116	T20604	T20605	auxpass	was,labeled
R16117	T20605	T20605	ROOT	labeled,labeled
R16118	T20606	T20605	prep	with,labeled
R16119	T20607	T20610	compound	[,UTP
R16120	T20608	T20610	compound	α-32P,UTP
R16121	T20609	T20610	compound	],UTP
R16122	T20610	T20606	pobj	UTP,with
R16123	T20611	T20605	advcl	using,labeled
R16124	T20612	T20614	nummod	T7,polymerase
R16125	T20613	T20614	compound	RNA,polymerase
R16126	T20614	T20611	dobj	polymerase,using
R16127	T20615	T20605	cc	and,labeled
R16128	T20616	T20605	conj	purified,labeled
R16129	T20617	T20616	xcomp	using,purified
R16130	T20618	T20619	compound	Sephadex,G-50
R16131	T20619	T20620	compound	G-50,columns
R16132	T20620	T20617	dobj	columns,using
R16133	T20621	T20620	punct	(,columns
R16134	T20622	T20623	compound	NucAway,Spin
R16135	T20623	T20624	compound	Spin,column
R16136	T20624	T20620	appos	column,columns
R16137	T20625	T20624	punct	",",column
R16138	T20626	T20624	appos	Ambion,column
R16139	T20627	T20624	punct	),column
R16140	T20628	T20605	punct	.,labeled
R16141	T20629	T20631	det	The,probe
R16142	T20630	T20631	amod	labeled,probe
R16143	T20631	T20633	nsubjpass	probe,quantitated
R16144	T20632	T20633	auxpass	was,quantitated
R16145	T20633	T20633	ROOT	quantitated,quantitated
R16146	T20634	T20633	advcl	using,quantitated
R16147	T20635	T20636	det	a,scintillation
R16148	T20636	T20634	dobj	scintillation,using
R16149	T20637	T20634	advmod	counter,using
R16150	T20638	T20634	punct	",",using
R16151	T20639	T20633	cc	and,quantitated
R16152	T20640	T20644	nummod	6,cpm
R16153	T20641	T20644	nmod	×,cpm
R16154	T20642	T20641	nummod	105,×
R16155	T20643	T20644	compound	cherenkov,cpm
R16156	T20644	T20646	nsubjpass	cpm,used
R16157	T20645	T20646	auxpass	was,used
R16158	T20646	T20633	conj	used,quantitated
R16159	T20647	T20648	aux	to,hybridize
R16160	T20648	T20646	xcomp	hybridize,used
R16161	T20649	T20648	prep	to,hybridize
R16162	T20650	T20651	nummod	4,μg
R16163	T20651	T20649	pobj	μg,to
R16164	T20652	T20651	prep	of,μg
R16165	T20653	T20654	amod	total,RNA
R16166	T20654	T20652	pobj	RNA,of
R16167	T20655	T20648	advcl	using,hybridize
R16168	T20656	T20658	det	the,kit
R16169	T20657	T20658	compound	RPA,kit
R16170	T20658	T20655	dobj	kit,using
R16171	T20659	T20658	punct	(,kit
R16172	T20660	T20661	compound	BD,Biosciences
R16173	T20661	T20658	appos	Biosciences,kit
R16174	T20662	T20658	punct	),kit
R16175	T20663	T20646	punct	.,used
R16176	T20664	T20666	nsubjpass	Samples,denatured
R16177	T20665	T20666	auxpass	were,denatured
R16178	T20666	T20666	ROOT	denatured,denatured
R16179	T20667	T20666	prep	at,denatured
R16180	T20668	T20669	nummod	90,°
R16181	T20669	T20667	pobj	°,at
R16182	T20670	T20666	conj	C,denatured
R16183	T20671	T20670	cc	and,C
R16184	T20672	T20670	conj	hybridized,C
R16185	T20673	T20672	prep	at,hybridized
R16186	T20674	T20676	nummod	56,C
R16187	T20675	T20676	nummod	°,C
R16188	T20676	T20677	compound	C,overnight
R16189	T20677	T20673	pobj	overnight,at
R16190	T20678	T20666	punct	.,denatured
R16191	T20679	T20680	compound	Single-stranded,RNA
R16192	T20680	T20682	nsubjpass	RNA,digested
R16193	T20681	T20682	auxpass	was,digested
R16194	T20682	T20682	ROOT	digested,digested
R16195	T20683	T20682	prep	with,digested
R16196	T20684	T20685	det	a,mixture
R16197	T20685	T20683	pobj	mixture,with
R16198	T20686	T20685	prep	of,mixture
R16199	T20687	T20688	compound	Rnase,A
R16200	T20688	T20686	pobj	A,of
R16201	T20689	T20688	cc	and,A
R16202	T20690	T20688	conj	T1,A
R16203	T20691	T20690	punct	",",T1
R16204	T20692	T20682	cc	and,digested
R16205	T20693	T20682	conj	precipitated,digested
R16206	T20694	T20693	xcomp	using,precipitated
R16207	T20695	T20694	dobj	isopropanol,using
R16208	T20696	T20682	punct	.,digested
R16209	T20697	T20698	amod	Protected,probes
R16210	T20698	T20700	nsubjpass	probes,resolved
R16211	T20699	T20700	auxpass	were,resolved
R16212	T20700	T20700	ROOT	resolved,resolved
R16213	T20701	T20700	prep	on,resolved
R16214	T20702	T20707	det	a,gel
R16215	T20703	T20707	amod	denaturing,gel
R16216	T20704	T20705	nummod	4.75,%
R16217	T20705	T20707	compound	%,gel
R16218	T20706	T20707	compound	acrylamide,gel
R16219	T20707	T20701	pobj	gel,on
R16220	T20708	T20709	punct	",",dried
R16221	T20709	T20700	conj	dried,resolved
R16222	T20710	T20709	punct	",",dried
R16223	T20711	T20709	cc	and,dried
R16224	T20712	T20709	conj	imaged,dried
R16225	T20713	T20712	xcomp	using,imaged
R16226	T20714	T20715	det	a,phosphorImager
R16227	T20715	T20713	dobj	phosphorImager,using
R16228	T20716	T20718	punct	(,Dynamics
R16229	T20717	T20718	compound	Molecular,Dynamics
R16230	T20718	T20715	appos	Dynamics,phosphorImager
R16231	T20719	T20715	punct	),phosphorImager
R16232	T20720	T20709	punct	.,dried
R16233	T20721	T20723	nsubjpass	Densitometry,performed
R16234	T20722	T20723	auxpass	was,performed
R16235	T20723	T20723	ROOT	performed,performed
R16236	T20724	T20723	advcl	using,performed
R16237	T20725	T20729	det	the,software
R16238	T20726	T20729	nmod	ImageQuant,software
R16239	T20727	T20729	nmod	TL,software
R16240	T20728	T20729	nummod	v2005,software
R16241	T20729	T20724	dobj	software,using
R16242	T20730	T20729	punct	(,software
R16243	T20731	T20732	compound	GE,Healthcare
R16244	T20732	T20729	appos	Healthcare,software
R16245	T20733	T20729	punct	),software
R16246	T20734	T20723	punct	.,performed
R16359	T20886	T20887	compound	PCR,analysis
R16360	T20887	T20887	ROOT	analysis,analysis
R16361	T20888	T20887	prep	of,analysis
R16362	T20889	T20891	compound	κ,rearrangement
R16363	T20890	T20891	compound	chain,rearrangement
R16364	T20891	T20888	pobj	rearrangement,of
R16365	T20892	T20887	punct	.,analysis
R16366	T20893	T20894	nsubj	DNA,was
R16367	T20894	T20894	ROOT	was,was
R16368	T20895	T20894	acomp	isolated,was
R16369	T20896	T20895	prep	from,isolated
R16370	T20897	T20896	pobj	splenocytes,from
R16371	T20898	T20894	advcl	using,was
R16372	T20899	T20902	det	the,Kit
R16373	T20900	T20902	compound	Dneasy,Kit
R16374	T20901	T20902	compound	Tissue,Kit
R16375	T20902	T20898	dobj	Kit,using
R16376	T20903	T20902	punct	(,Kit
R16377	T20904	T20902	appos	Qiagen,Kit
R16378	T20905	T20902	punct	),Kit
R16379	T20906	T20898	punct	",",using
R16380	T20907	T20898	prep	with,using
R16381	T20908	T20909	compound	Rnase,treatment
R16382	T20909	T20907	pobj	treatment,with
R16383	T20910	T20894	punct	.,was
R16384	T20911	T20912	compound	PCR,reactions
R16385	T20912	T20913	nsubj	reactions,contained
R16386	T20913	T20913	ROOT	contained,contained
R16387	T20914	T20915	nummod	100,ng
R16388	T20915	T20913	dobj	ng,contained
R16389	T20916	T20915	prep	of,ng
R16390	T20917	T20918	amod	genomic,DNA
R16391	T20918	T20916	pobj	DNA,of
R16392	T20919	T20915	punct	",",ng
R16393	T20920	T20921	nummod	0.2,μM
R16394	T20921	T20915	appos	μM,ng
R16395	T20922	T20923	det	each,primer
R16396	T20923	T20915	conj	primer,ng
R16397	T20924	T20923	punct	",",primer
R16398	T20925	T20927	nummod	0.2,dNTPs
R16399	T20926	T20927	compound	mM,dNTPs
R16400	T20927	T20923	appos	dNTPs,primer
R16401	T20928	T20927	punct	",",dNTPs
R16402	T20929	T20927	cc	and,dNTPs
R16403	T20930	T20934	nummod	2.5,polymerase
R16404	T20931	T20934	compound	U,polymerase
R16405	T20932	T20934	compound	Taq,polymerase
R16406	T20933	T20934	compound	DNA,polymerase
R16407	T20934	T20938	nsubj	polymerase,performed
R16408	T20935	T20936	punct	(,NEB
R16409	T20936	T20934	appos	NEB,polymerase
R16410	T20937	T20934	punct	),polymerase
R16411	T20938	T20915	relcl	performed,ng
R16412	T20939	T20938	prep	in,performed
R16413	T20940	T20942	compound	1X,buffer
R16414	T20941	T20942	compound	ThermoPol,buffer
R16415	T20942	T20939	pobj	buffer,in
R16416	T20943	T20944	punct	(,NEB
R16417	T20944	T20942	appos	NEB,buffer
R16418	T20945	T20944	punct	),NEB
R16419	T20946	T20913	punct	.,contained
R16420	T20947	T20949	nsubj	Primers,were
R16421	T20948	T20947	acl	used,Primers
R16422	T20949	T20949	ROOT	were,were
R16423	T20950	T20949	attr	Vκcon,were
R16424	T20951	T20950	cc	and,Vκcon
R16425	T20952	T20950	conj	Jκ5,Vκcon
R16426	T20953	T20950	nummod	1,Vκcon
R16427	T20954	T20950	appos	°,Vκcon
R16428	T20955	T20949	cc	and,were
R16429	T20956	T20958	aux	have,described
R16430	T20957	T20958	auxpass	been,described
R16431	T20958	T20949	conj	described,were
R16432	T20959	T20962	advmod	previously,]
R16433	T20960	T20962	nmod	[,]
R16434	T20961	T20962	nummod	74,]
R16435	T20962	T20958	dobj	],described
R16436	T20963	T20958	punct	.,described
R16437	T20964	T20965	compound	PCR,conditions
R16438	T20965	T20966	nsubj	conditions,were
R16439	T20966	T20966	ROOT	were,were
R16440	T20967	T20968	nummod	94,°
R16441	T20968	T20969	compound	°,C
R16442	T20969	T20966	attr	C,were
R16443	T20970	T20969	prep	for,C
R16444	T20971	T20972	nummod	2,min
R16445	T20972	T20970	pobj	min,for
R16446	T20973	T20972	punct	",",min
R16447	T20974	T20975	nummod	40,cycles
R16448	T20975	T20972	appos	cycles,min
R16449	T20976	T20975	prep	of,cycles
R16450	T20977	T20978	compound	94,°
R16451	T20978	T20979	nummod	°,C
R16452	T20979	T20976	pobj	C,of
R16453	T20980	T20969	prep	for,C
R16454	T20981	T20987	preconj	30,for
R16455	T20982	T20981	nmod	s,30
R16456	T20983	T20982	punct	",",s
R16457	T20984	T20985	nummod	63,°
R16458	T20985	T20986	compound	°,C
R16459	T20986	T20982	appos	C,s
R16460	T20987	T20966	prep	for,were
R16461	T20988	T20989	nummod	90,s
R16462	T20989	T20987	pobj	s,for
R16463	T20990	T20987	punct	",",for
R16464	T20991	T20987	cc	and,for
R16465	T20992	T20994	nummod	72,C
R16466	T20993	T20994	compound	°,C
R16467	T20994	T20987	conj	C,for
R16468	T20995	T20994	prep	for,C
R16469	T20996	T20997	nummod	1,min
R16470	T20997	T20995	pobj	min,for
R16471	T20998	T20994	punct	",",C
R16472	T20999	T20966	advcl	followed,were
R16473	T21000	T20999	agent	by,followed
R16474	T21001	T21002	nummod	1,cycle
R16475	T21002	T21000	pobj	cycle,by
R16476	T21003	T21002	prep	of,cycle
R16477	T21004	T21005	compound	72,°
R16478	T21005	T21006	nummod	°,C
R16479	T21006	T21003	pobj	C,of
R16480	T21007	T20999	prep	for,followed
R16481	T21008	T21009	nummod	5,min
R16482	T21009	T21007	pobj	min,for
R16483	T21010	T20966	punct	.,were
R16646	T21195	T21196	compound	Immunoblot,analysis
R16647	T21196	T21196	ROOT	analysis,analysis
R16648	T21197	T21196	punct	.,analysis
R16649	T21198	T21199	amod	Whole,cell
R16650	T21199	T21200	compound	cell,lysates
R16651	T21200	T21202	nsubjpass	lysates,prepared
R16652	T21201	T21202	auxpass	were,prepared
R16653	T21202	T21202	ROOT	prepared,prepared
R16654	T21203	T21202	prep	in,prepared
R16655	T21204	T21205	compound	radioimmunoprecipitation,assay
R16656	T21205	T21203	pobj	assay,in
R16657	T21206	T21205	punct	(,assay
R16658	T21207	T21209	nmod	RIPA,buffer
R16659	T21208	T21209	punct	),buffer
R16660	T21209	T21205	appos	buffer,assay
R16661	T21210	T21215	punct	(,pH
R16662	T21211	T21215	nummod	20,pH
R16663	T21212	T21215	compound	mM,pH
R16664	T21213	T21215	compound	Tris-HCl,pH
R16665	T21214	T21215	compound	[,pH
R16666	T21215	T21217	nmod	pH,]
R16667	T21216	T21217	nummod	7.5,]
R16668	T21217	T21209	appos	],buffer
R16669	T21218	T21217	punct	",",]
R16670	T21219	T21221	nummod	150,NaCl
R16671	T21220	T21221	compound	mM,NaCl
R16672	T21221	T21217	appos	NaCl,]
R16673	T21222	T21221	punct	",",NaCl
R16674	T21223	T21225	nummod	1,EDTA
R16675	T21224	T21225	compound	mM,EDTA
R16676	T21225	T21221	appos	EDTA,NaCl
R16677	T21226	T21225	punct	",",EDTA
R16678	T21227	T21228	nummod	1,%
R16679	T21228	T21229	compound	%,NP-40
R16680	T21229	T21225	appos	NP-40,EDTA
R16681	T21230	T21229	punct	",",NP-40
R16682	T21231	T21232	nummod	0.1,%
R16683	T21232	T21233	compound	%,SDS
R16684	T21233	T21217	appos	SDS,]
R16685	T21234	T21233	punct	",",SDS
R16686	T21235	T21236	nummod	0.1,%
R16687	T21236	T21238	nmod	%,deoxycholate
R16688	T21237	T21238	compound	sodium,deoxycholate
R16689	T21238	T21233	appos	deoxycholate,SDS
R16690	T21239	T21205	punct	),assay
R16691	T21240	T21202	conj	supplemented,prepared
R16692	T21241	T21240	prep	with,supplemented
R16693	T21242	T21245	nummod	2,fluoride
R16694	T21243	T21245	nmod	mM,fluoride
R16695	T21244	T21245	compound	phenylmethylsulfonyl,fluoride
R16696	T21245	T21241	pobj	fluoride,with
R16697	T21246	T21245	punct	",",fluoride
R16698	T21247	T21249	nummod	1,Na3VO4
R16699	T21248	T21249	compound	mM,Na3VO4
R16700	T21249	T21245	appos	Na3VO4,fluoride
R16701	T21250	T21249	punct	",",Na3VO4
R16702	T21251	T21249	cc	and,Na3VO4
R16703	T21252	T21255	nummod	1:100,cocktails
R16704	T21253	T21255	amod	protease/phosphatase,cocktails
R16705	T21254	T21255	compound	inhibitor,cocktails
R16706	T21255	T21249	conj	cocktails,Na3VO4
R16707	T21256	T21255	punct	(,cocktails
R16708	T21257	T21255	appos	Sigma,cocktails
R16709	T21258	T21255	punct	),cocktails
R16710	T21259	T21202	punct	.,prepared
R16711	T21260	T21261	amod	Crude,lysates
R16712	T21261	T21263	nsubjpass	lysates,centrifuged
R16713	T21262	T21263	auxpass	were,centrifuged
R16714	T21263	T21263	ROOT	centrifuged,centrifuged
R16715	T21264	T21263	prep	at,centrifuged
R16716	T21265	T21266	nummod	"13,000",rpm
R16717	T21266	T21264	pobj	rpm,at
R16718	T21267	T21263	prep	for,centrifuged
R16719	T21268	T21269	nummod	10,min
R16720	T21269	T21267	pobj	min,for
R16721	T21270	T21263	prep	at,centrifuged
R16722	T21271	T21272	nummod	4,°
R16723	T21272	T21273	nummod	°,C
R16724	T21273	T21270	pobj	C,at
R16725	T21274	T21273	cc	and,C
R16726	T21275	T21276	det	the,supernatants
R16727	T21276	T21273	conj	supernatants,C
R16728	T21277	T21278	auxpass	were,collected
R16729	T21278	T21263	conj	collected,centrifuged
R16730	T21279	T21278	prep	for,collected
R16731	T21280	T21281	amod	further,analysis
R16732	T21281	T21279	pobj	analysis,for
R16733	T21282	T21263	punct	.,centrifuged
R16734	T21283	T21284	compound	Protein,concentrations
R16735	T21284	T21286	nsubjpass	concentrations,determined
R16736	T21285	T21286	auxpass	were,determined
R16748	T21297	T21297	ROOT	boiled,boiled
R16749	T21298	T21297	prep	in,boiled
R16750	T21299	T21300	det	the,presence
R16751	T21300	T21298	pobj	presence,in
R16752	T21301	T21300	prep	of,presence
R16753	T21302	T21303	nummod	2.5,%
R16754	T21303	T21304	compound	%,β-mecaptoethanol
R16755	T21304	T21301	pobj	β-mecaptoethanol,of
R16756	T21305	T21304	punct	",",β-mecaptoethanol
R16757	T21306	T21300	acl	separated,presence
R16758	T21307	T21306	agent	by,separated
R16759	T21308	T21309	compound	denaturing,SDS-PAGE
R16760	T21309	T21307	pobj	SDS-PAGE,by
R16761	T21310	T21297	punct	",",boiled
R16762	T21311	T21297	cc	and,boiled
R16763	T21312	T21297	conj	transferred,boiled
R16764	T21313	T21312	prep	to,transferred
R16765	T21314	T21316	dep	0.45,μm
R16766	T21315	T21316	punct	-,μm
R16767	T21316	T21318	compound	μm,membranes
R16768	T21317	T21318	compound	Optitran,membranes
R16769	T21318	T21313	pobj	membranes,to
R16770	T21319	T21318	punct	(,membranes
R16771	T21320	T21318	appos	Schleicher,membranes
R16772	T21321	T21320	cc	&,Schleicher
R16773	T21322	T21320	conj	Schuell,Schleicher
R16774	T21323	T21318	punct	),membranes
R16775	T21324	T21312	prep	in,transferred
R16776	T21325	T21328	det	a,unit
R16777	T21326	T21328	compound	Bio-Rad,unit
R16778	T21327	T21328	compound	transfer,unit
R16779	T21328	T21324	pobj	unit,in
R16780	T21329	T21297	punct	.,boiled
R16781	T21330	T21332	nsubjpass	Membranes,immunoblotted
R16782	T21331	T21332	auxpass	were,immunoblotted
R16783	T21332	T21342	ccomp	immunoblotted,tagged
R16784	T21333	T21332	prep	with,immunoblotted
R16785	T21334	T21337	det	the,antibody
R16786	T21335	T21337	amod	appropriate,antibody
R16787	T21336	T21337	amod	primary,antibody
R16788	T21337	T21333	pobj	antibody,with
R16789	T21338	T21337	acl	followed,antibody
R16790	T21339	T21338	agent	by,followed
R16791	T21340	T21341	compound	horseradish,peroxidase
R16792	T21341	T21339	pobj	peroxidase,by
R16793	T21342	T21342	ROOT	tagged,tagged
R16794	T21343	T21344	amod	secondary,antibodies
R16795	T21344	T21342	dobj	antibodies,tagged
R16796	T21345	T21352	punct	(,detected
R16797	T21346	T21347	compound	Amersham,Biosciences
R16798	T21347	T21352	nsubj	Biosciences,detected
R16799	T21348	T21347	cc	and,Biosciences
R16800	T21349	T21347	conj	Dako,Biosciences
R16801	T21350	T21349	punct	),Dako
R16802	T21351	T21347	cc	and,Biosciences
R16803	T21352	T21342	parataxis	detected,tagged
R16804	T21353	T21352	prep	with,detected
R16805	T21354	T21358	det	the,System
R16806	T21355	T21358	compound	SuperSignal,System
R16807	T21356	T21358	compound	West,System
R16808	T21357	T21358	compound	Pico,System
R16809	T21358	T21353	pobj	System,with
R16810	T21359	T21358	punct	(,System
R16811	T21360	T21358	appos	Pierce,System
R16812	T21361	T21358	punct	),System
R16813	T21362	T21342	punct	.,tagged
R16814	T21474	T21474	ROOT	Antibodies,Antibodies
R16815	T21475	T21474	punct	.,Antibodies
R16816	T21476	T21477	amod	FITC-conjugated,goat
R16817	T21477	T21479	compound	goat,IgM
R16818	T21478	T21479	compound	anti-mouse,IgM
R16819	T21479	T21479	ROOT	IgM,IgM
R16820	T21480	T21479	punct	",",IgM
R16821	T21481	T21484	compound	rat,IgD
R16822	T21482	T21484	compound	IgG2aκ,IgD
R16823	T21483	T21484	compound	anti-mouse,IgD
R16824	T21484	T21479	appos	IgD,IgM
R16825	T21485	T21486	punct	(,clone
R16832	T21492	T21494	nmod	IgG1κ,kappa
R16833	T21493	T21494	compound	anti-mouse,kappa
R16834	T21494	T21486	appos	kappa,clone
R16835	T21495	T21496	punct	(,clone
R16836	T21496	T21494	appos	clone,kappa
R16837	T21497	T21496	nummod	187.1,clone
R16838	T21498	T21496	punct	),clone
R16839	T21499	T21494	punct	",",kappa
R16840	T21500	T21510	npadvmod	rat,PE-conjugated
R16841	T21501	T21503	nmod	IgG2bκ,lambda
R16842	T21502	T21503	amod	anti-mouse,lambda
R16843	T21503	T21510	ccomp	lambda,PE-conjugated
R16844	T21504	T21506	punct	(,JC5
R16845	T21505	T21506	compound	clone,JC5
R16846	T21506	T21503	appos	JC5,lambda
R16847	T21507	T21510	meta	1,PE-conjugated
R16848	T21508	T21507	punct	),1
R16849	T21509	T21510	punct	;,PE-conjugated
R16850	T21510	T21510	ROOT	PE-conjugated,PE-conjugated
R16851	T21511	T21512	compound	goat,anti-mouse
R16852	T21512	T21513	advmod	anti-mouse,IgG
R16853	T21513	T21510	dobj	IgG,PE-conjugated
R16854	T21514	T21513	punct	;,IgG
R16855	T21515	T21518	amod	un-conjugated,kappa
R16856	T21516	T21518	compound	goat,kappa
R16857	T21517	T21518	compound	anti-mouse,kappa
R16858	T21518	T21513	appos	kappa,IgG
R16859	T21519	T21518	cc	and,kappa
R16860	T21520	T21518	conj	anti-mouse,kappa
R16861	T21521	T21523	nsubjpass	lambda,purchased
R16862	T21522	T21523	auxpass	were,purchased
R16863	T21523	T21510	conj	purchased,PE-conjugated
R16864	T21524	T21523	prep	from,purchased
R16865	T21525	T21526	compound	Southern,Biotech
R16866	T21526	T21524	pobj	Biotech,from
R16867	T21527	T21510	punct	.,PE-conjugated
R16868	T21528	T21529	amod	APC-conjugated,rat
R16869	T21529	T21532	compound	rat,CD19
R16870	T21530	T21532	compound	IgG2aκ,CD19
R16871	T21531	T21532	compound	anti-mouse,CD19
R16872	T21532	T21532	ROOT	CD19,CD19
R16873	T21533	T21534	punct	(,clone
R16874	T21534	T21535	compound	clone,6D5
R16875	T21535	T21532	appos	6D5,CD19
R16876	T21536	T21532	punct	),CD19
R16877	T21537	T21532	punct	",",CD19
R16878	T21538	T21539	amod	PE-conjugated,mouse
R16879	T21539	T21541	compound	mouse,anti-LMP1
R16880	T21540	T21541	compound	IgG2aκ,anti-LMP1
R16881	T21541	T21532	conj	anti-LMP1,CD19
R16882	T21542	T21543	punct	(,clone
R16883	T21543	T21544	compound	clone,S12
R16884	T21544	T21541	appos	S12,anti-LMP1
R16885	T21545	T21541	punct	),anti-LMP1
R16886	T21546	T21541	punct	",",anti-LMP1
R16887	T21547	T21548	amod	un-conjugated,mouse
R16888	T21548	T21550	compound	mouse,anti-Rb
R16889	T21549	T21550	compound	IgG2a,anti-Rb
R16890	T21550	T21541	conj	anti-Rb,anti-LMP1
R16891	T21551	T21552	punct	(,clone
R16892	T21552	T21550	parataxis	clone,anti-Rb
R16893	T21553	T21552	nummod	2,clone
R16894	T21554	T21553	punct	),2
R16895	T21555	T21552	punct	",",clone
R16896	T21556	T21552	cc	and,clone
R16897	T21557	T21552	conj	anti-Cdk2,clone
R16898	T21558	T21563	punct	(,purchased
R16899	T21559	T21561	nmod	clone,)
R16900	T21560	T21559	nummod	55,clone
R16901	T21561	T21563	punct	),purchased
R16902	T21562	T21563	auxpass	were,purchased
R16903	T21563	T21563	ROOT	purchased,purchased
R16904	T21564	T21563	prep	from,purchased
R16905	T21565	T21566	compound	BD,Bioscience
R16906	T21566	T21564	pobj	Bioscience,from
R16907	T21567	T21563	punct	.,purchased
R16908	T21568	T21569	amod	PE-conjugated,rat
R16909	T21569	T21572	nmod	rat,CD5
R16910	T21570	T21572	nmod	IgG2aκ,CD5
R16911	T21571	T21572	compound	anti-mouse,CD5
R16912	T21572	T21579	nsubjpass	CD5,purchased
R16913	T21573	T21574	punct	(,clone
R16914	T21574	T21572	appos	clone,CD5
R16915	T21575	T21574	nummod	53,clone
R16916	T21576	T21574	appos	7.3,clone
R16917	T21577	T21574	punct	),clone
R16918	T21578	T21579	auxpass	was,purchased
R16919	T21579	T21579	ROOT	purchased,purchased
R16920	T21580	T21579	prep	from,purchased
R16921	T21581	T21580	pobj	eBioscience,from
R16922	T21582	T21579	punct	.,purchased
R16923	T21583	T21584	compound	Rat,anti-LMP1
R16924	T21584	T21597	nsubjpass	anti-LMP1,purchased
R16925	T21585	T21597	punct	(,purchased
R16926	T21586	T21597	nsubjpass	clones,purchased
R16927	T21587	T21586	dobj	8G3,clones
R16928	T21588	T21587	punct	",",8G3
R16929	T21589	T21587	conj	1G6,8G3
R16930	T21590	T21589	punct	",",1G6
R16931	T21591	T21589	conj	7E10,1G6
R16932	T21592	T21591	punct	",",7E10
R16933	T21593	T21591	cc	and,7E10
R16934	T21594	T21591	conj	7G8,7E10
R16935	T21595	T21597	punct	),purchased
R16936	T21596	T21597	auxpass	was,purchased
R16937	T21597	T21597	ROOT	purchased,purchased
R16938	T21598	T21597	prep	from,purchased
R16939	T21599	T21598	pobj	Ascenion,from
R16940	T21600	T21597	punct	.,purchased
R16941	T21601	T21602	compound	Rabbit,anti-pAkt
R16942	T21602	T21638	nsubj	anti-pAkt,purchased
R16943	T21603	T21609	punct	(,β
R16944	T21604	T21609	amod	Ser473,β
R16945	T21605	T21604	punct	),Ser473
R16946	T21606	T21609	punct	",",β
R16947	T21607	T21609	amod	anti-pGSK3α,β
R16948	T21608	T21609	compound	/,β
R16949	T21609	T21602	appos	β,anti-pAkt
R16950	T21610	T21609	punct	(,β
R16951	T21611	T21609	appos	Ser21/9,β
R16952	T21612	T21609	punct	),β
R16953	T21613	T21609	punct	",",β
R16954	T21614	T21638	nmod	anti-pStat3,purchased
R16955	T21615	T21616	punct	(,Tyr705
R16956	T21616	T21614	appos	Tyr705,anti-pStat3
R16957	T21617	T21614	punct	),anti-pStat3
R16958	T21618	T21614	punct	",",anti-pStat3
R16959	T21619	T21614	conj	anti-pStat6,anti-pStat3
R16960	T21620	T21619	punct	(,anti-pStat6
R16961	T21621	T21619	appos	Tyr641,anti-pStat6
R16962	T21622	T21619	punct	),anti-pStat6
R16963	T21623	T21619	punct	",",anti-pStat6
R16964	T21624	T21614	appos	anti-pmTOR,anti-pStat3
R16965	T21625	T21624	punct	(,anti-pmTOR
R16966	T21626	T21624	appos	Ser2448,anti-pmTOR
R16967	T21627	T21624	punct	),anti-pmTOR
R16968	T21628	T21624	punct	",",anti-pmTOR
R16969	T21629	T21638	amod	anti-Stat6,purchased
R16970	T21630	T21629	punct	",",anti-Stat6
R16971	T21631	T21638	nsubjpass	anti-Akt,purchased
R16972	T21632	T21631	punct	",",anti-Akt
R16973	T21633	T21631	conj	anti-FoxO1,anti-Akt
R16974	T21634	T21633	punct	",",anti-FoxO1
R16975	T21635	T21633	cc	and,anti-FoxO1
R16976	T21636	T21633	conj	anti-p27,anti-FoxO1
R16977	T21637	T21638	auxpass	were,purchased
R16978	T21638	T21638	ROOT	purchased,purchased
R16979	T21639	T21638	prep	from,purchased
R16980	T21640	T21641	compound	Cell,Signaling
R16981	T21641	T21639	pobj	Signaling,from
R16982	T21642	T21638	punct	.,purchased
R16983	T21643	T21644	compound	Rabbit,anti-Stat3
R16984	T21644	T21662	nsubjpass	anti-Stat3,purchased
R16985	T21645	T21646	punct	(,H-190
R16986	T21646	T21644	appos	H-190,anti-Stat3
R16987	T21647	T21646	punct	),H-190
R16988	T21648	T21644	punct	",",anti-Stat3
R16989	T21649	T21662	nsubjpass	anti-IκBα,purchased
R16990	T21650	T21651	punct	(,C-21
R16991	T21651	T21649	appos	C-21,anti-IκBα
R16992	T21652	T21651	punct	),C-21
R16993	T21653	T21649	punct	",",anti-IκBα
R16994	T21654	T21649	cc	and,anti-IκBα
R16995	T21655	T21657	nmod	goat,actin
R16996	T21656	T21657	amod	anti-β,actin
R16997	T21657	T21649	conj	actin,anti-IκBα
R16998	T21658	T21659	punct	(,I-19
R16999	T21659	T21657	appos	I-19,actin
R17000	T21660	T21657	punct	),actin
R17001	T21661	T21662	auxpass	were,purchased
R17002	T21662	T21662	ROOT	purchased,purchased
R17003	T21663	T21662	prep	from,purchased
R17004	T21664	T21665	compound	Santa,Cruz
R17005	T21665	T21666	compound	Cruz,Biotechnology
R17006	T21666	T21663	pobj	Biotechnology,from
R17007	T21667	T21662	punct	.,purchased
R17008	T21668	T21670	compound	Mouse,anti-GSK3
R17009	T21669	T21670	compound	IgG1κ,anti-GSK3
R17010	T21670	T21672	nsubjpass	anti-GSK3,purchased
R17011	T21671	T21672	auxpass	was,purchased
R17012	T21672	T21672	ROOT	purchased,purchased
R17013	T21673	T21672	prep	from,purchased
R17014	T21674	T21675	compound	Upstate,Biotechnology
R17015	T21675	T21673	pobj	Biotechnology,from
R17016	T21676	T21672	punct	.,purchased
R17017	T21677	T21678	compound	Rabbit,anti-pRb
R17018	T21678	T21678	ROOT	anti-pRb,anti-pRb
R17019	T21679	T21683	punct	(,purchased
R17020	T21680	T21683	nsubjpass	Thr373,purchased
R17021	T21681	T21680	punct	),Thr373
R17022	T21682	T21683	auxpass	was,purchased
R17023	T21683	T21683	ROOT	purchased,purchased
R17024	T21684	T21683	prep	from,purchased
R17025	T21685	T21686	compound	EMD,Biosciences
R17026	T21686	T21684	pobj	Biosciences,from
R17027	T21687	T21683	punct	.,purchased
R15830	T20283	T20284	compound	Becton,Dickinson
R15831	T20284	T20281	appos	Dickinson,program
R15832	T20285	T20281	punct	),program
R15833	T20286	T20275	punct	.,performed
R15834	T20287	T20288	amod	Further,analysis
R14973	T19323	T19322	punct	",",4
R14974	T19324	T19322	amod	5-dimethylthiazol-2-yl,4
R14975	T19325	T19322	punct	),4
R14976	T19326	T19322	punct	-5,4
R14977	T19327	T19322	punct	-,4
R14978	T19328	T19329	punct	(,3-carboxymethoxyphenyl
R14979	T19329	T19322	appos	3-carboxymethoxyphenyl,4
R14980	T19330	T19322	punct	),4
R14981	T19331	T19322	punct	-2,4
R14982	T19332	T19331	punct	-,-2
R14983	T19333	T19331	punct	(,-2
R14984	T19334	T19331	appos	4-sulfophenyl,-2
R14985	T19335	T19331	punct	),-2
R14986	T19336	T19339	nummod	-2,salt
R14987	T19337	T19339	nmod	H-tetrazolium,salt
R14988	T19338	T19339	amod	inner,salt
R14989	T19339	T19345	nmod	salt,assays
R14990	T19340	T19341	punct	(,MTS
R14991	T19341	T19345	nmod	MTS,assays
R14992	T19342	T19341	punct	),MTS
R14993	T19343	T19345	compound	cell,assays
R14994	T19344	T19345	compound	cytotoxicity/proliferation,assays
R14995	T19345	T19347	nsubjpass	assays,performed
R14996	T19346	T19347	auxpass	were,performed
R14997	T19347	T19347	ROOT	performed,performed
R14998	T19348	T19347	advcl	using,performed
R14999	T19349	T19356	det	the,assay
R15000	T19350	T19355	nmod	CellTiter,proliferation
R15001	T19351	T19352	nummod	96,aqueous
R15002	T19352	T19355	nmod	aqueous,proliferation
R15003	T19353	T19355	amod	one-solution,proliferation
R15100	T19450	T19449	pobj	day,on
R15101	T19451	T19450	prep	of,day
R15102	T19452	T19451	pobj	harvest,of
R15103	T19453	T19452	cc	and,harvest
R15104	T19454	T19452	conj	IL4,harvest
R15105	T19455	T19454	punct	",",IL4
R15106	T19456	T19459	compound	rat,IL4
R15107	T19457	T19459	compound	IgG1,IL4
R15108	T19458	T19459	compound	anti-mouse,IL4
R15109	T19459	T19454	conj	IL4,IL4
R15110	T19460	T19459	punct	",",IL4
R15111	T19461	T19459	cc	or,IL4
R15112	T19462	T19465	nmod	rat,control
R15113	T19463	T19465	amod	IgG1,control
R15114	T19464	T19465	compound	isotype,control
R15115	T19465	T19459	conj	control,IL4
R15116	T19466	T19468	punct	(,Systems
R15117	T19467	T19468	compound	R&D,Systems
R15118	T19468	T19465	appos	Systems,control
R15119	T19469	T19438	punct	),seeded
R15120	T19470	T19471	auxpass	were,added
R15121	T19471	T19475	csubj	added,indicated
R15122	T19472	T19471	prep	at,added
R15123	T19473	T19474	det	the,concentrations
R15124	T19474	T19472	pobj	concentrations,at
R15125	T19475	T19438	dep	indicated,seeded
R15126	T19476	T19475	prep	in,indicated
R15127	T19477	T19478	det	the,figures
R15128	T19478	T19476	pobj	figures,in
R15129	T19479	T19475	punct	.,indicated
R15130	T19480	T19482	nsubjpass	Cultures,pulsed
R15131	T19481	T19482	auxpass	were,pulsed
R15132	T19482	T19482	ROOT	pulsed,pulsed
R15133	T19483	T19482	prep	for,pulsed
R15134	T19484	T19485	nummod	4,h
R15135	T19485	T19483	pobj	h,for
R15136	T19486	T19485	prep	with,h
R15137	T19487	T19488	compound	MTS,reagent
R15138	T19488	T19486	pobj	reagent,with
R15139	T19489	T19488	nummod	1,reagent
R15140	T19490	T19491	meta	d,post-seeding
R15141	T19491	T19491	ROOT	post-seeding,post-seeding
R15142	T19492	T19491	punct	.,post-seeding
R15143	T19493	T19499	prep	For,seeded
R15144	T19494	T19495	compound	inhibitor,studies
R15145	T19495	T19493	pobj	studies,For
R15146	T19496	T19499	punct	",",seeded
R15147	T19497	T19499	nsubjpass	splenocytes,seeded
R15207	T19557	T19558	auxpass	was,induced
R15521	T19934	T19933	punct	(,IgG
R15522	T19935	T19933	appos	H+L,IgG
R15523	T19936	T19933	punct	),IgG
R15524	T19937	T19922	conj	pre-adsorbed,μg
R15525	T19938	T19939	aux	to,mouse
R15526	T19939	T19940	compound	mouse,serum
R15527	T19940	T19920	pobj	serum,with
R15528	T19941	T19940	punct	(,serum
R15529	T19942	T19943	compound	Jackson,ImmunoResearch
R15758	T20211	T20213	nsubjpass	kit,used
R15759	T20212	T20213	auxpass	was,used
R15760	T20213	T20213	ROOT	used,used
R15761	T20214	T20215	mark	as,instructed
R15762	T20215	T20213	advcl	instructed,used
R15763	T20216	T20215	agent	by,instructed
R15764	T20217	T20218	det	the,manufacturer
R15765	T20218	T20216	pobj	manufacturer,by
R15766	T20219	T20221	punct	(,Bioscience
R15767	T20220	T20221	compound	BD,Bioscience
R15768	T20221	T20218	appos	Bioscience,manufacturer
R15769	T20222	T20213	punct	),used
R15770	T20223	T20213	punct	.,used
R15771	T20224	T20228	advmod	Briefly,exposed
R15772	T20225	T20228	punct	",",exposed
R15773	T20226	T20228	nsubjpass	cells,exposed
R15774	T20227	T20228	auxpass	were,exposed
R15775	T20228	T20228	ROOT	exposed,exposed
R15776	T20229	T20228	prep	to,exposed
R15777	T20230	T20229	pobj	EMA,to
R15778	T20231	T20233	punct	(,Probes
R15779	T20232	T20233	compound	Molecular,Probes
R15780	T20233	T20230	appos	Probes,EMA
R15781	T20234	T20228	punct	),exposed
R15782	T20235	T20228	prep	for,exposed
R15783	T20236	T20235	pobj	exclusion,for
R15812	T20265	T20262	cc	and,treated
R15813	T20266	T20262	conj	stained,treated
R15814	T20267	T20266	prep	with,stained
R15818	T20271	T20262	punct	.,treated
R15819	T20272	T20273	compound	Flow,cytometry
R15820	T20273	T20275	nsubjpass	cytometry,performed
R15821	T20274	T20275	auxpass	was,performed
R15822	T20275	T20275	ROOT	performed,performed
R15823	T20276	T20275	prep	on,performed
R15824	T20277	T20276	pobj	FACScalibur,on
R15825	T20278	T20275	advcl	using,performed
R15826	T20279	T20281	det	the,program
R15827	T20280	T20281	compound	CellQuest,program
R15828	T20281	T20278	dobj	program,using
R15829	T20282	T20281	punct	(,program
R15835	T20288	T20290	nsubjpass	analysis,conducted
R15836	T20289	T20290	auxpass	was,conducted
R15837	T20290	T20290	ROOT	conducted,conducted
R15838	T20291	T20290	prep	on,conducted
R15839	T20292	T20296	det	the,program
R15840	T20293	T20296	nmod	Summit,program
R15841	T20294	T20296	nummod	v4,program
R16737	T21286	T21286	ROOT	determined,determined
R16738	T21287	T21286	prep	with,determined
R16739	T21288	T21293	det	the,system
R16740	T21289	T21290	compound	Bio-Rad,DC
R16741	T21290	T21293	compound	DC,system
R16742	T21291	T21293	compound	protein,system
R16743	T21292	T21293	compound	assay,system
R16744	T21293	T21287	pobj	system,with
R16745	T21294	T21286	punct	.,determined
R16746	T21295	T21297	nsubjpass	Lysates,boiled
R16747	T21296	T21297	auxpass	were,boiled
R16826	T21486	T21510	dep	clone,PE-conjugated
R16827	T21487	T21486	nummod	11,clone
R16828	T21488	T21486	appos	26,clone
R16829	T21489	T21486	punct	),clone
R16830	T21490	T21486	punct	",",clone
R16831	T21491	T21486	intj	rat,clone

UBERON-AE

Id	Subject	Object	Predicate	Lexical cue
T18283	269-273	http://purl.obolibrary.org/obo/UBERON_0002415	denotes	tail
T18284	319-324	http://purl.obolibrary.org/obo/UBERON_0002107	denotes	liver
T18285	622-629	http://purl.obolibrary.org/obo/UBERON_0000916	denotes	abdomen
T18601	952-958	http://purl.obolibrary.org/obo/UBERON_0002106	denotes	spleen
T18602	959-965	http://purl.obolibrary.org/obo/UBERON_0000479	denotes	tissue
T18603	1365-1370	http://purl.obolibrary.org/obo/UBERON_0001977	denotes	serum
T19635	3371-3377	http://purl.obolibrary.org/obo/UBERON_0002106	denotes	spleen
T19636	3830-3836	http://purl.obolibrary.org/obo/UBERON_0000479	denotes	tissue
T19637	3948-3953	http://purl.obolibrary.org/obo/UBERON_0001977	denotes	serum
T20766	6179-6185	http://purl.obolibrary.org/obo/UBERON_0000479	denotes	Tissue

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T18294	595-606	http://purl.obolibrary.org/obo/GO_0032502	denotes	development
T18616	891-897	http://purl.obolibrary.org/obo/GO_0040007	denotes	growth
T19048	2132-2150	http://purl.obolibrary.org/obo/GO_0008283	denotes	cell proliferation
T19049	3168-3183	http://purl.obolibrary.org/obo/GO_0001775	denotes	cell activation
T19050	3168-3195	http://purl.obolibrary.org/obo/GO_0071888	denotes	cell activation was induced
T19649	4013-4024	http://purl.obolibrary.org/obo/GO_0016791	denotes	phosphatase
T20343	5294-5307	http://purl.obolibrary.org/obo/GO_0006351	denotes	Transcription
T21033	6882-6893	http://purl.obolibrary.org/obo/GO_0016791	denotes	phosphatase

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T20009	4013-4024	http://purl.obolibrary.org/obo/GO_0016791	denotes	phosphatase
T20010	4194-4202	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T20308	4380-4388	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T20309	4852-4860	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T21369	6882-6893	http://purl.obolibrary.org/obo/GO_0016791	denotes	phosphatase
T21370	7377-7385	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T21371	7438-7448	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T20310	4380-4388	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T20311	4852-4860	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T20312	4380-4388	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T20313	4852-4860	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T20314	4432-4435	http://purl.obolibrary.org/obo/GO_0005680	denotes	APC
T20315	4591-4596	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T20316	4658-4663	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T20317	4779-4784	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T20744	5095-5100	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T20745	5677-5680	http://purl.obolibrary.org/obo/GO_0005662	denotes	RPA
T19601	2034-2038	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T18992	903-908	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18993	1179-1184	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18994	1480-1485	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18995	1615-1620	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T18996	1736-1740	http://purl.obolibrary.org/obo/GO_0071735	denotes	IgG1
T18997	1765-1769	http://purl.obolibrary.org/obo/GO_0071735	denotes	IgG1
T19602	2132-2136	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T19603	3168-3172	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T19604	2638-2642	http://purl.obolibrary.org/obo/GO_0071735	denotes	IgG1
T19605	2666-2670	http://purl.obolibrary.org/obo/GO_0071735	denotes	IgG1
T20011	4194-4202	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T20012	4194-4202	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T20013	4212-4216	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell
T21372	6625-6629	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T21373	7258-7267	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T21374	7319-7328	http://purl.obolibrary.org/obo/GO_0016020	denotes	Membranes
T21375	7377-7385	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T21376	7438-7448	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T21377	7377-7385	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T21378	7438-7448	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T21691	7850-7853	http://purl.obolibrary.org/obo/GO_0005680	denotes	APC
T21692	7975-7980	http://purl.obolibrary.org/obo/GO_0071735	denotes	IgG2a
T21693	8408-8412	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell

sentences

Id	Subject	Object	Predicate	Lexical cue
T18288	23-39	Sentence	denotes	Transgenic mice.
T18289	40-206	Sentence	denotes	Generation of LMP1 mice under the Ig heavy chain promoter and enhancer have been described previously and were maintained as heterozygotes on a Balbc background [26].
T18290	207-307	Sentence	denotes	LMP1 mice were genotyped by Southern blot and PCR analysis of tail DNA as described previously [26].
T18291	308-483	Sentence	denotes	Spleen and liver sections were fixed in 4% paraformaldehyde and embedded in paraffin, and 5-μm sections were stained with hematoxylin and eosin for histopathological analysis.
T18292	484-630	Sentence	denotes	Lymphomas were passaged by intraperitoneal injection of 1 × 108 splenocytes into SCID mice and sacrificed upon development of an extended abdomen.
T18293	631-875	Sentence	denotes	Animals were housed in the Association for Assessment and Accreditation for Animal Care–approved animal facility at the University of North Carolina at Chapel Hill. All protocols were approved by the Institutional Animal Care and Use Committee.
T18609	877-909	Sentence	denotes	Isolation and growth of B cells.
T18610	910-1045	Sentence	denotes	Splenocytes were prepared by homogenizing spleen tissue with two frosted slides and debris was filtered through a 100-μm cell strainer.
T18611	1046-1176	Sentence	denotes	Erythrocytes were lysed using 0.8% ammonium chloride solution (StemCell Technologies) for 10 min on ice and washed twice with PBS.
T18612	1177-1406	Sentence	denotes	B cells were isolated using CD19-MACS beads according to the manufacturer's instructions (Miltenyi Biotec) and grown in Iscove's medium supplemented with heat-inactivated 10% fetal bovine serum and antibiotic/antimycotic (GIBCO).
T18613	1407-1576	Sentence	denotes	Splenocytes isolated from SCID-passaged lymphomas consisted of 80%–90% B cells as determined by flow cytometry, and were hence not further purified with CD19-MACS beads.
T18614	1577-1812	Sentence	denotes	Splenocytes were seeded at 1.25 × 106 cells/ml and where applicable, recombinant mouse IL4 was added at 100 ng/ml, recombinant mouse IL10 at 10 ng/ml, and rat IgG1 anti-mouse IL10 and rat IgG1 isotype control at 10 μg/ml (R&D Systems).
T18615	1813-1910	Sentence	denotes	For BrdU incorporation assays, splenocytes were pulsed for 24 h with 10 μM BrdU 1 d post-harvest.
T19038	1912-1922	Sentence	denotes	MTS assay.
T19039	1923-2209	Sentence	denotes	The 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) cell cytotoxicity/proliferation assays were performed using the CellTiter 96 aqueous one-solution cell proliferation assay (Promega), according to manufacturer's instructions.
T19040	2210-2305	Sentence	denotes	For IL4 studies, splenocytes were cultured for 3 d in the presence or absence of 100 ng/ml IL4.
T19041	2306-2407	Sentence	denotes	Cells were seeded on day 3 in triplicate in a 96-well plate at 2.5 × 106 cells/ml at 100 μl per well.
T19042	2408-2516	Sentence	denotes	MTS reagent was added for 4 h and absorbance was read at 540 nm; values plotted were subtracted from blanks.
T19043	2517-2759	Sentence	denotes	For neutralization assays, splenocytes were seeded at 5 × 106 cells/ml at 100 μl per well on day of harvest and IL4, rat IgG1 anti-mouse IL4, or rat IgG1 isotype control (R&D Systems) were added at the concentrations indicated in the figures.
T19044	2760-2823	Sentence	denotes	Cultures were pulsed for 4 h with MTS reagent 1 d post-seeding.
T19045	2824-3002	Sentence	denotes	For inhibitor studies, splenocytes were seeded at 1 × 107 cells/ml at 100 μl per well on day of harvest, and inhibitors were added at the concentrations indicated in the figures.
T19046	3003-3265	Sentence	denotes	The inhibitors BAY11–7085, rapamycin, triciribine, U0126, SB202190, and cucurbitacin I were purchased from EMD Biosciences. For non-malignant splenocyte cultures, B cell activation was induced with 10 μg/ml of goat F(ab') anti-mouse IgM (Jackson ImmunoResearch).
T19047	3266-3329	Sentence	denotes	Cultures were pulsed for 4 h with MTS reagent 1 d post-seeding.
T19642	3331-3352	Sentence	denotes	Immunohistochemistry.
T19643	3353-3477	Sentence	denotes	Paraffin-embedded spleen sections were deparaffinized in Histoclear (National Diagnostics) and rehydrated in graded ethanol.
T19644	3478-3608	Sentence	denotes	Sections were antigen retrieved by microwaving in citrate buffer (pH 6.0) for 15 min (LMP1 staining) and 10 min (pStat3 staining).
T19645	3609-3748	Sentence	denotes	For LMP1 staining, sections were blocked with 1% BSA and 0.1% cold fish skin gelatin followed with streptavidin/biotin block (Vector Labs).
T19646	3749-4060	Sentence	denotes	Rat IgG anti-LMP1 (clones 8G3 and 1G6, Ascenion) were used at 1:10 dilution from tissue culture supernatants, followed with 8 μg/ml biotinylated mouse F(ab') anti-rat IgG (H+L) pre-adsorbed to mouse serum (Jackson ImmunoResearch) and 2 μg/ml streptavidin-alkaline phosphatase conjugate (Jackson ImmunoResearch).
T19647	4061-4143	Sentence	denotes	Stains were developed with BCIP/NBT and counterstained in Nuclear Fast Red (Dako).
T19648	4144-4296	Sentence	denotes	Phospho-Stat3 was detected with 4 μg/ml of pStat3 antibody (Tyr705, Cell Signaling) and detected with anti-rabbit Poly-HRP IHC detection kit (Chemicon).
T20038	4298-4313	Sentence	denotes	Flow cytometry.
T20039	4314-4478	Sentence	denotes	One million splenocytes were stained with the appropriate primary antibody unconjugated or conjugated to FITC, PE, or APC diluted in stain buffer (PBS with 3% FBS).
T20040	4479-4581	Sentence	denotes	For BrdU detection, the FITC-BrdU flow kit was used as instructed by the manufacturer (BD Bioscience).
T20041	4582-4753	Sentence	denotes	Briefly, cells were exposed to EMA (Molecular Probes) for exclusion of dead cells, stained for surface antigens, fixed in paraformaldehyde, and permeabilized with saponin.
T20042	4754-4861	Sentence	denotes	To expose BrdU epitopes, cells were treated with Dnase and stained with FITC-conjugated anti-BrdU antibody.
T20043	4862-4953	Sentence	denotes	Flow cytometry was performed on FACScalibur using the CellQuest program (Becton Dickinson).
T20044	4954-5019	Sentence	denotes	Further analysis was conducted on the Summit v4.2 program (Dako).
T20334	5021-5044	Sentence	denotes	Rnase protection assay.
T20335	5045-5216	Sentence	denotes	Total RNA was isolated from CD19+ MACS-purified B cells using the Rneasy midi purification kit with Dnase treatment, according to the manufacturer's instructions (Qiagen).
T20336	5217-5374	Sentence	denotes	The mCK-1 probe template set (BD Biosciences) was labeled using the In Vitro Transcription Kit according to the manufacturer's instructions (BD Biosciences).
T20337	5375-5532	Sentence	denotes	Briefly, 50 ng of mCK-1 probe set was labeled with [α-32P]UTP using T7 RNA polymerase and purified using Sephadex G-50 columns (NucAway Spin column, Ambion).
T20338	5533-5702	Sentence	denotes	The labeled probe was quantitated using a scintillation counter, and 6 × 105 cherenkov cpm was used to hybridize to 4 μg of total RNA using the RPA kit (BD Biosciences).
T20339	5703-5769	Sentence	denotes	Samples were denatured at 90 °C and hybridized at 56 °C overnight.
T20340	5770-5872	Sentence	denotes	Single-stranded RNA was digested with a mixture of Rnase A and T1, and precipitated using isopropanol.
T20341	5873-6004	Sentence	denotes	Protected probes were resolved on a denaturing 4.75% acrylamide gel, dried, and imaged using a phosphorImager (Molecular Dynamics).
T20342	6005-6087	Sentence	denotes	Densitometry was performed using the ImageQuant TL v2005 software (GE Healthcare).
T20768	6089-6127	Sentence	denotes	PCR analysis of κ chain rearrangement.
T20769	6128-6221	Sentence	denotes	DNA was isolated from splenocytes using the Dneasy Tissue Kit (Qiagen), with Rnase treatment.
T20770	6222-6377	Sentence	denotes	PCR reactions contained 100 ng of genomic DNA, 0.2 μM each primer, 0.2 mM dNTPs, and 2.5 U Taq DNA polymerase (NEB) performed in 1X ThermoPol buffer (NEB).
T20771	6378-6453	Sentence	denotes	Primers used were Vκcon and Jκ5–1° and have been described previously [74].
T20772	6454-6596	Sentence	denotes	PCR conditions were 94 °C for 2 min, 40 cycles of 94 °C for 30 s, 63 °C for 90 s, and 72 °C for 1 min, followed by 1 cycle of 72 °C for 5 min.
T21027	6598-6618	Sentence	denotes	Immunoblot analysis.
T21028	6619-6922	Sentence	denotes	Whole cell lysates were prepared in radioimmunoprecipitation assay (RIPA) buffer (20 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.1% sodium deoxycholate) supplemented with 2 mM phenylmethylsulfonyl fluoride, 1 mM Na3VO4, and 1:100 protease/phosphatase inhibitor cocktails (Sigma).
T21029	6923-7044	Sentence	denotes	Crude lysates were centrifuged at 13,000 rpm for 10 min at 4 °C and the supernatants were collected for further analysis.
T21030	7045-7125	Sentence	denotes	Protein concentrations were determined with the Bio-Rad DC protein assay system.
T21031	7126-7318	Sentence	denotes	Lysates were boiled in the presence of 2.5% β-mecaptoethanol, separated by denaturing SDS-PAGE, and transferred to 0.45-μm Optitran membranes (Schleicher & Schuell) in a Bio-Rad transfer unit.
T21032	7319-7541	Sentence	denotes	Membranes were immunoblotted with the appropriate primary antibody followed by horseradish peroxidase–tagged secondary antibodies (Amersham Biosciences and Dako) and detected with the SuperSignal West Pico System (Pierce).
T21448	7543-7554	Sentence	denotes	Antibodies.
T21449	7555-8146	Sentence	denotes	FITC-conjugated goat anti-mouse IgM, rat IgG2aκ anti-mouse IgD (clone 11–26), rat IgG1κ anti-mouse kappa (clone 187.1), rat IgG2bκ anti-mouse lambda (clone JC5–1); PE-conjugated goat anti-mouse IgG; un-conjugated goat anti-mouse kappa and anti-mouse lambda were purchased from Southern Biotech. APC-conjugated rat IgG2aκ anti-mouse CD19 (clone 6D5), PE-conjugated mouse IgG2aκ anti-LMP1 (clone S12), un-conjugated mouse IgG2a anti-Rb (clone 2), and anti-Cdk2 (clone 55) were purchased from BD Bioscience. PE-conjugated rat IgG2aκ anti-mouse CD5 (clone 53–7.3) was purchased from eBioscience.
T21450	8147-8668	Sentence	denotes	Rat anti-LMP1 (clones 8G3, 1G6, 7E10, and 7G8) was purchased from Ascenion. Rabbit anti-pAkt (Ser473), anti-pGSK3α/β (Ser21/9), anti-pStat3 (Tyr705), anti-pStat6 (Tyr641), anti-pmTOR (Ser2448), anti-Stat6, anti-Akt, anti-FoxO1, and anti-p27 were purchased from Cell Signaling. Rabbit anti-Stat3 (H-190), anti-IκBα (C-21), and goat anti-β actin (I-19) were purchased from Santa Cruz Biotechnology. Mouse IgG1κ anti-GSK3 was purchased from Upstate Biotechnology. Rabbit anti-pRb (Thr373) was purchased from EMD Biosciences.
T287	0-21	Sentence	denotes	Materials and Methods
T288	23-39	Sentence	denotes	Transgenic mice.
T289	40-206	Sentence	denotes	Generation of LMP1 mice under the Ig heavy chain promoter and enhancer have been described previously and were maintained as heterozygotes on a Balbc background [26].
T290	207-307	Sentence	denotes	LMP1 mice were genotyped by Southern blot and PCR analysis of tail DNA as described previously [26].
T291	308-483	Sentence	denotes	Spleen and liver sections were fixed in 4% paraformaldehyde and embedded in paraffin, and 5-μm sections were stained with hematoxylin and eosin for histopathological analysis.
T292	484-630	Sentence	denotes	Lymphomas were passaged by intraperitoneal injection of 1 × 108 splenocytes into SCID mice and sacrificed upon development of an extended abdomen.
T293	631-795	Sentence	denotes	Animals were housed in the Association for Assessment and Accreditation for Animal Care–approved animal facility at the University of North Carolina at Chapel Hill.
T294	796-875	Sentence	denotes	All protocols were approved by the Institutional Animal Care and Use Committee.
T295	877-909	Sentence	denotes	Isolation and growth of B cells.
T296	910-1045	Sentence	denotes	Splenocytes were prepared by homogenizing spleen tissue with two frosted slides and debris was filtered through a 100-μm cell strainer.
T297	1046-1176	Sentence	denotes	Erythrocytes were lysed using 0.8% ammonium chloride solution (StemCell Technologies) for 10 min on ice and washed twice with PBS.
T298	1177-1406	Sentence	denotes	B cells were isolated using CD19-MACS beads according to the manufacturer's instructions (Miltenyi Biotec) and grown in Iscove's medium supplemented with heat-inactivated 10% fetal bovine serum and antibiotic/antimycotic (GIBCO).
T299	1407-1576	Sentence	denotes	Splenocytes isolated from SCID-passaged lymphomas consisted of 80%–90% B cells as determined by flow cytometry, and were hence not further purified with CD19-MACS beads.
T300	1577-1812	Sentence	denotes	Splenocytes were seeded at 1.25 × 106 cells/ml and where applicable, recombinant mouse IL4 was added at 100 ng/ml, recombinant mouse IL10 at 10 ng/ml, and rat IgG1 anti-mouse IL10 and rat IgG1 isotype control at 10 μg/ml (R&D Systems).
T301	1813-1910	Sentence	denotes	For BrdU incorporation assays, splenocytes were pulsed for 24 h with 10 μM BrdU 1 d post-harvest.
T302	1912-1922	Sentence	denotes	MTS assay.
T303	1923-2209	Sentence	denotes	The 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) cell cytotoxicity/proliferation assays were performed using the CellTiter 96 aqueous one-solution cell proliferation assay (Promega), according to manufacturer's instructions.
T304	2210-2305	Sentence	denotes	For IL4 studies, splenocytes were cultured for 3 d in the presence or absence of 100 ng/ml IL4.
T305	2306-2407	Sentence	denotes	Cells were seeded on day 3 in triplicate in a 96-well plate at 2.5 × 106 cells/ml at 100 μl per well.
T306	2408-2516	Sentence	denotes	MTS reagent was added for 4 h and absorbance was read at 540 nm; values plotted were subtracted from blanks.
T307	2517-2759	Sentence	denotes	For neutralization assays, splenocytes were seeded at 5 × 106 cells/ml at 100 μl per well on day of harvest and IL4, rat IgG1 anti-mouse IL4, or rat IgG1 isotype control (R&D Systems) were added at the concentrations indicated in the figures.
T308	2760-2823	Sentence	denotes	Cultures were pulsed for 4 h with MTS reagent 1 d post-seeding.
T309	2824-3002	Sentence	denotes	For inhibitor studies, splenocytes were seeded at 1 × 107 cells/ml at 100 μl per well on day of harvest, and inhibitors were added at the concentrations indicated in the figures.
T310	3003-3126	Sentence	denotes	The inhibitors BAY11–7085, rapamycin, triciribine, U0126, SB202190, and cucurbitacin I were purchased from EMD Biosciences.
T311	3127-3265	Sentence	denotes	For non-malignant splenocyte cultures, B cell activation was induced with 10 μg/ml of goat F(ab') anti-mouse IgM (Jackson ImmunoResearch).
T312	3266-3329	Sentence	denotes	Cultures were pulsed for 4 h with MTS reagent 1 d post-seeding.
T313	3331-3352	Sentence	denotes	Immunohistochemistry.
T314	3353-3477	Sentence	denotes	Paraffin-embedded spleen sections were deparaffinized in Histoclear (National Diagnostics) and rehydrated in graded ethanol.
T315	3478-3608	Sentence	denotes	Sections were antigen retrieved by microwaving in citrate buffer (pH 6.0) for 15 min (LMP1 staining) and 10 min (pStat3 staining).
T316	3609-3748	Sentence	denotes	For LMP1 staining, sections were blocked with 1% BSA and 0.1% cold fish skin gelatin followed with streptavidin/biotin block (Vector Labs).
T317	3749-4060	Sentence	denotes	Rat IgG anti-LMP1 (clones 8G3 and 1G6, Ascenion) were used at 1:10 dilution from tissue culture supernatants, followed with 8 μg/ml biotinylated mouse F(ab') anti-rat IgG (H+L) pre-adsorbed to mouse serum (Jackson ImmunoResearch) and 2 μg/ml streptavidin-alkaline phosphatase conjugate (Jackson ImmunoResearch).
T318	4061-4143	Sentence	denotes	Stains were developed with BCIP/NBT and counterstained in Nuclear Fast Red (Dako).
T319	4144-4296	Sentence	denotes	Phospho-Stat3 was detected with 4 μg/ml of pStat3 antibody (Tyr705, Cell Signaling) and detected with anti-rabbit Poly-HRP IHC detection kit (Chemicon).
T320	4298-4313	Sentence	denotes	Flow cytometry.
T321	4314-4478	Sentence	denotes	One million splenocytes were stained with the appropriate primary antibody unconjugated or conjugated to FITC, PE, or APC diluted in stain buffer (PBS with 3% FBS).
T322	4479-4581	Sentence	denotes	For BrdU detection, the FITC-BrdU flow kit was used as instructed by the manufacturer (BD Bioscience).
T323	4582-4753	Sentence	denotes	Briefly, cells were exposed to EMA (Molecular Probes) for exclusion of dead cells, stained for surface antigens, fixed in paraformaldehyde, and permeabilized with saponin.
T324	4754-4861	Sentence	denotes	To expose BrdU epitopes, cells were treated with Dnase and stained with FITC-conjugated anti-BrdU antibody.
T325	4862-4953	Sentence	denotes	Flow cytometry was performed on FACScalibur using the CellQuest program (Becton Dickinson).
T326	4954-5019	Sentence	denotes	Further analysis was conducted on the Summit v4.2 program (Dako).
T327	5021-5044	Sentence	denotes	Rnase protection assay.
T328	5045-5216	Sentence	denotes	Total RNA was isolated from CD19+ MACS-purified B cells using the Rneasy midi purification kit with Dnase treatment, according to the manufacturer's instructions (Qiagen).
T329	5217-5374	Sentence	denotes	The mCK-1 probe template set (BD Biosciences) was labeled using the In Vitro Transcription Kit according to the manufacturer's instructions (BD Biosciences).
T330	5375-5532	Sentence	denotes	Briefly, 50 ng of mCK-1 probe set was labeled with [α-32P]UTP using T7 RNA polymerase and purified using Sephadex G-50 columns (NucAway Spin column, Ambion).
T331	5533-5702	Sentence	denotes	The labeled probe was quantitated using a scintillation counter, and 6 × 105 cherenkov cpm was used to hybridize to 4 μg of total RNA using the RPA kit (BD Biosciences).
T332	5703-5769	Sentence	denotes	Samples were denatured at 90 °C and hybridized at 56 °C overnight.
T333	5770-5872	Sentence	denotes	Single-stranded RNA was digested with a mixture of Rnase A and T1, and precipitated using isopropanol.
T334	5873-6004	Sentence	denotes	Protected probes were resolved on a denaturing 4.75% acrylamide gel, dried, and imaged using a phosphorImager (Molecular Dynamics).
T335	6005-6087	Sentence	denotes	Densitometry was performed using the ImageQuant TL v2005 software (GE Healthcare).
T336	6089-6127	Sentence	denotes	PCR analysis of κ chain rearrangement.
T337	6128-6221	Sentence	denotes	DNA was isolated from splenocytes using the Dneasy Tissue Kit (Qiagen), with Rnase treatment.
T338	6222-6377	Sentence	denotes	PCR reactions contained 100 ng of genomic DNA, 0.2 μM each primer, 0.2 mM dNTPs, and 2.5 U Taq DNA polymerase (NEB) performed in 1X ThermoPol buffer (NEB).
T339	6378-6453	Sentence	denotes	Primers used were Vκcon and Jκ5–1° and have been described previously [74].
T340	6454-6596	Sentence	denotes	PCR conditions were 94 °C for 2 min, 40 cycles of 94 °C for 30 s, 63 °C for 90 s, and 72 °C for 1 min, followed by 1 cycle of 72 °C for 5 min.
T341	6598-6618	Sentence	denotes	Immunoblot analysis.
T342	6619-6922	Sentence	denotes	Whole cell lysates were prepared in radioimmunoprecipitation assay (RIPA) buffer (20 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.1% sodium deoxycholate) supplemented with 2 mM phenylmethylsulfonyl fluoride, 1 mM Na3VO4, and 1:100 protease/phosphatase inhibitor cocktails (Sigma).
T343	6923-7044	Sentence	denotes	Crude lysates were centrifuged at 13,000 rpm for 10 min at 4 °C and the supernatants were collected for further analysis.
T344	7045-7125	Sentence	denotes	Protein concentrations were determined with the Bio-Rad DC protein assay system.
T345	7126-7318	Sentence	denotes	Lysates were boiled in the presence of 2.5% β-mecaptoethanol, separated by denaturing SDS-PAGE, and transferred to 0.45-μm Optitran membranes (Schleicher & Schuell) in a Bio-Rad transfer unit.
T346	7319-7541	Sentence	denotes	Membranes were immunoblotted with the appropriate primary antibody followed by horseradish peroxidase–tagged secondary antibodies (Amersham Biosciences and Dako) and detected with the SuperSignal West Pico System (Pierce).
T347	7543-7554	Sentence	denotes	Antibodies.
T348	7555-7849	Sentence	denotes	FITC-conjugated goat anti-mouse IgM, rat IgG2aκ anti-mouse IgD (clone 11–26), rat IgG1κ anti-mouse kappa (clone 187.1), rat IgG2bκ anti-mouse lambda (clone JC5–1); PE-conjugated goat anti-mouse IgG; un-conjugated goat anti-mouse kappa and anti-mouse lambda were purchased from Southern Biotech.
T349	7850-8059	Sentence	denotes	APC-conjugated rat IgG2aκ anti-mouse CD19 (clone 6D5), PE-conjugated mouse IgG2aκ anti-LMP1 (clone S12), un-conjugated mouse IgG2a anti-Rb (clone 2), and anti-Cdk2 (clone 55) were purchased from BD Bioscience.
T350	8060-8146	Sentence	denotes	PE-conjugated rat IgG2aκ anti-mouse CD5 (clone 53–7.3) was purchased from eBioscience.
T351	8147-8222	Sentence	denotes	Rat anti-LMP1 (clones 8G3, 1G6, 7E10, and 7G8) was purchased from Ascenion.
T352	8223-8423	Sentence	denotes	Rabbit anti-pAkt (Ser473), anti-pGSK3α/β (Ser21/9), anti-pStat3 (Tyr705), anti-pStat6 (Tyr641), anti-pmTOR (Ser2448), anti-Stat6, anti-Akt, anti-FoxO1, and anti-p27 were purchased from Cell Signaling.
T353	8424-8543	Sentence	denotes	Rabbit anti-Stat3 (H-190), anti-IκBα (C-21), and goat anti-β actin (I-19) were purchased from Santa Cruz Biotechnology.
T354	8544-8607	Sentence	denotes	Mouse IgG1κ anti-GSK3 was purchased from Upstate Biotechnology.
T355	8608-8668	Sentence	denotes	Rabbit anti-pRb (Thr373) was purchased from EMD Biosciences.

events-check-again

Id	Subject	Object	Predicate	Lexical cue
T18589	54-58	Protein	denotes	LMP1
T18590	207-211	Protein	denotes	LMP1
T19021	1205-1209	Protein	denotes	CD19
T19022	1560-1564	Protein	denotes	CD19
T19023	1664-1667	Protein	denotes	IL4
T19024	1710-1714	Protein	denotes	IL10
T19025	1752-1756	Protein	denotes	IL10
T19622	2214-2217	Protein	denotes	IL4
T19623	2301-2304	Protein	denotes	IL4
T19624	2629-2632	Protein	denotes	IL4
T19625	2654-2657	Protein	denotes	IL4
T20034	3564-3568	Protein	denotes	LMP1
T20035	3613-3617	Protein	denotes	LMP1
T20036	3762-3766	Protein	denotes	LMP1
T20037	4152-4157	Protein	denotes	Stat3
T20761	5073-5077	Protein	denotes	CD19
T20762	5821-5828	Protein	denotes	Rnase A
T20763	5833-5835	Protein	denotes	T1
T21023	6313-6331	Protein	denotes	Taq DNA polymerase
T21384	7410-7420	Protein	denotes	peroxidase
T21758	7887-7891	Protein	denotes	CD19
T21759	7937-7941	Protein	denotes	LMP1
T21760	7986-7988	Protein	denotes	Rb
T21761	8009-8013	Protein	denotes	Cdk2
T21762	8096-8099	Protein	denotes	CD5
T21763	8156-8160	Protein	denotes	LMP1
T21764	8235-8239	Protein	denotes	pAkt
T21765	8255-8261	Protein	denotes	pGSK3α
T21766	8262-8263	Protein	denotes	β
T21767	8280-8286	Protein	denotes	pStat3
T21768	8302-8308	Protein	denotes	pStat6
T21769	8324-8329	Protein	denotes	pmTOR
T21770	8346-8351	Protein	denotes	Stat6
T21771	8358-8361	Protein	denotes	Akt
T21772	8368-8373	Protein	denotes	FoxO1
T21773	8384-8387	Protein	denotes	p27
T21774	8436-8441	Protein	denotes	Stat3
T21775	8456-8460	Protein	denotes	IκBα
T21776	8483-8490	Protein	denotes	β actin
T21777	8561-8565	Protein	denotes	GSK3
T21778	8620-8623	Protein	denotes	pRb

bionlp-st-ge-2016-reference-tees

Id	Subject	Object	Predicate	Lexical cue
T19008	1172-1175	Protein	denotes	PBS
T20030	4093-4096	Protein	denotes	NBT
T20031	4144-4151	Protein	denotes	Phospho
T20032	4152-4157	Protein	denotes	Stat3
T20033	4187-4193	Protein	denotes	pStat3
T20318	4360-4388	Protein	denotes	appropriate primary antibody
T20319	4483-4487	Protein	denotes	BrdU
T20320	4488-4497	Gene_expression	denotes	detection
T20321	4745-4752	Protein	denotes	saponin
T20322	4764-4768	Protein	denotes	BrdU
T20323	4803-4808	Protein	denotes	Dnase
T20324	4847-4851	Protein	denotes	BrdU
T20752	5021-5026	Protein	denotes	Rnase
T20753	5073-5077	Protein	denotes	CD19
T20754	5079-5083	Protein	denotes	MACS
T20755	5145-5150	Protein	denotes	Dnase
T20756	5221-5226	Protein	denotes	mCK-1
T20757	5393-5408	Protein	denotes	mCK-1 probe set
T20758	5443-5460	Protein	denotes	T7 RNA polymerase
T20759	5511-5515	Protein	denotes	Spin
T20760	5821-5826	Protein	denotes	Rnase
T21018	6105-6126	Protein	denotes	κ chain rearrangement
T21019	6205-6210	Protein	denotes	Rnase
T21020	6313-6331	Protein	denotes	Taq DNA polymerase
T21021	6333-6336	Protein	denotes	NEB
T21022	6406-6412	Protein	denotes	Jκ5–1°
T21381	6867-6893	Protein	denotes	1:100 protease/phosphatase
T21382	7097-7100	Protein	denotes	Rad
T21383	7398-7420	Protein	denotes	horseradish peroxidase
T21736	7587-7590	Protein	denotes	IgM
T21737	7592-7617	Protein	denotes	rat IgG2aκ anti-mouse IgD
T21738	7633-7659	Protein	denotes	rat IgG1κ anti-mouse kappa
T21739	7675-7685	Protein	denotes	rat IgG2bκ
T21740	7749-7752	Protein	denotes	IgG
T21741	7850-7891	Protein	denotes	APC-conjugated rat IgG2aκ anti-mouse CD19
T21742	7919-7936	Protein	denotes	mouse IgG2aκ anti
T21743	7937-7941	Protein	denotes	LMP1
T21744	7975-7980	Protein	denotes	IgG2a
T21745	8009-8013	Protein	denotes	Cdk2
T21746	8078-8099	Protein	denotes	IgG2aκ anti-mouse CD5
T21747	8147-8160	Protein	denotes	Rat anti-LMP1
T21748	8250-8263	Protein	denotes	anti-pGSK3α/β
T21749	8341-8351	Protein	denotes	anti-Stat6
T21750	8353-8361	Protein	denotes	anti-Akt
T21751	8363-8373	Protein	denotes	anti-FoxO1
T21752	8384-8387	Protein	denotes	p27
T21753	8424-8441	Protein	denotes	Rabbit anti-Stat3
T21754	8451-8460	Protein	denotes	anti-IκBα
T21755	8473-8490	Protein	denotes	goat anti-β actin
T21756	8544-8560	Protein	denotes	Mouse IgG1κ anti
T21757	8561-8565	Protein	denotes	GSK3
T19015	1732-1756	Protein	denotes	rat IgG1 anti-mouse IL10
T18583	54-58	Protein	denotes	LMP1
T18584	74-110	Protein	denotes	Ig heavy chain promoter and enhancer
T18585	207-211	Protein	denotes	LMP1
T18586	384-392	Protein	denotes	paraffin
T18587	430-441	Protein	denotes	hematoxylin
T18588	446-451	Protein	denotes	eosin
T19009	1205-1209	Protein	denotes	CD19
T19010	1210-1214	Protein	denotes	MACS
T19011	1560-1564	Protein	denotes	CD19
T19012	1565-1569	Protein	denotes	MACS
T19013	1664-1667	Protein	denotes	IL4
T19014	1710-1714	Protein	denotes	IL10
T19016	1761-1777	Protein	denotes	rat IgG1 isotype
T19017	1778-1785	Regulation	denotes	control
T19018	1778-1785	Regulation	denotes	control
T19019	1817-1821	Protein	denotes	BrdU
T19020	1885-1894	Protein	denotes	μM BrdU 1
T19614	2214-2217	Protein	denotes	IL4
T19615	2301-2304	Protein	denotes	IL4
T19616	2629-2632	Protein	denotes	IL4
T19617	2634-2678	Protein	denotes	rat IgG1 anti-mouse IL4, or rat IgG1 isotype
T19618	3075-3089	Protein	denotes	cucurbitacin I
T19619	3213-3239	Protein	denotes	goat F(ab') anti-mouse IgM
T19620	3241-3248	Protein	denotes	Jackson
T19621	3188-3195	Positive_regulation	denotes	induced
T20022	3559-3562	Protein	denotes	min
T20023	3564-3568	Protein	denotes	LMP1
T20024	3586-3589	Protein	denotes	min
T20025	3591-3597	Protein	denotes	pStat3
T20026	3613-3617	Protein	denotes	LMP1
T20027	3749-3766	Protein	denotes	Rat IgG anti-LMP1
T20028	3991-4024	Protein	denotes	streptavidin-alkaline phosphatase
T20029	4088-4092	Protein	denotes	BCIP
R14698	T19015	T19017	themeOf	rat IgG1 anti-mouse IL10,control
R14699	T19016	T19018	themeOf	rat IgG1 isotype,control
R15241	T19619	T19621	themeOf	goat F(ab') anti-mouse IgM,induced
R15848	T20319	T20320	themeOf	BrdU,detection

bionlp-st-ge-2016-reference

Id	Subject	Object	Predicate	Lexical cue
T19638	3564-3568	Protein	denotes	LMP1
T19639	3613-3617	Protein	denotes	LMP1
T19640	3762-3766	Protein	denotes	LMP1
T19641	4152-4157	Protein	denotes	Stat3
T20767	6313-6331	Protein	denotes	Taq DNA polymerase
T20331	5073-5077	Protein	denotes	CD19
T20332	5821-5828	Protein	denotes	Rnase A
T20333	5833-5835	Protein	denotes	T1
T18286	54-58	Protein	denotes	LMP1
T18287	207-211	Protein	denotes	LMP1
T21026	7410-7420	Protein	denotes	peroxidase
T19034	2214-2217	Protein	denotes	IL4
T19035	2301-2304	Protein	denotes	IL4
T19036	2629-2632	Protein	denotes	IL4
T19037	2654-2657	Protein	denotes	IL4
T18604	1205-1209	Protein	denotes	CD19
T18605	1560-1564	Protein	denotes	CD19
T18606	1664-1667	Protein	denotes	IL4
T18607	1710-1714	Protein	denotes	IL10
T18608	1752-1756	Protein	denotes	IL10
T21427	7887-7891	Protein	denotes	CD19
T21428	7937-7941	Protein	denotes	LMP1
T21429	7986-7988	Protein	denotes	Rb
T21430	8009-8013	Protein	denotes	Cdk2
T21431	8096-8099	Protein	denotes	CD5
T21432	8156-8160	Protein	denotes	LMP1
T21433	8235-8239	Protein	denotes	pAkt
T21434	8255-8261	Protein	denotes	pGSK3α
T21435	8262-8263	Protein	denotes	β
T21436	8280-8286	Protein	denotes	pStat3
T21437	8302-8308	Protein	denotes	pStat6
T21438	8324-8329	Protein	denotes	pmTOR
T21439	8346-8351	Protein	denotes	Stat6
T21440	8358-8361	Protein	denotes	Akt
T21441	8368-8373	Protein	denotes	FoxO1
T21442	8384-8387	Protein	denotes	p27
T21443	8436-8441	Protein	denotes	Stat3
T21444	8456-8460	Protein	denotes	IκBα
T21445	8483-8490	Protein	denotes	β actin
T21446	8561-8565	Protein	denotes	GSK3
T21447	8620-8623	Protein	denotes	pRb

bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T18795	1205-1209	P15391	denotes	CD19
T18796	1560-1564	P15391	denotes	CD19
T18797	1664-1667	P05112	denotes	IL4
T18798	1710-1714	P22301	denotes	IL10
T18799	1752-1756	P22301	denotes	IL10
T19311	2214-2217	P05112	denotes	IL4
T19312	2301-2304	P05112	denotes	IL4
T19313	2629-2632	P05112	denotes	IL4
T19314	2654-2657	P05112	denotes	IL4
T19817	3544-3546	P0A7Z4	denotes	pH
T19818	3564-3568	P03230	denotes	LMP1
T19819	3591-3597	P40763	denotes	pStat3
T19820	3613-3617	P03230	denotes	LMP1
T19821	3762-3766	P03230	denotes	LMP1
T19822	4152-4157	P40763	denotes	Stat3
T19823	4187-4193	P40763	denotes	pStat3
T21451	7887-7891	P15391	denotes	CD19
T21452	7937-7941	P03230	denotes	LMP1
T21453	7986-7988	P06400	denotes	Rb
T21454	8009-8013	P24941	denotes	Cdk2
T21455	8096-8099	P06127	denotes	CD5
T21456	8156-8160	P03230	denotes	LMP1
T21457	8235-8239	Q9Y243	denotes	pAkt
T21458	8235-8239	P31749	denotes	pAkt
T21459	8235-8239	P31751	denotes	pAkt
T21460	8280-8286	P40763	denotes	pStat3
T21461	8302-8308	P42226	denotes	pStat6
T21462	8346-8351	P42226	denotes	Stat6
T21463	8358-8361	Q9Y243	denotes	Akt
T21464	8358-8361	P31749	denotes	Akt
T21465	8358-8361	P31751	denotes	Akt
T21466	8368-8373	Q12778	denotes	FoxO1
T21467	8384-8387	P46527	denotes	p27
T21468	8436-8441	P40763	denotes	Stat3
T21469	8456-8460	P25963	denotes	IκBα
T21470	8483-8490	P60709	denotes	β actin
T21471	8561-8565	P49840	denotes	GSK3
T21472	8561-8565	P49841	denotes	GSK3
T21473	8620-8623	P06400	denotes	pRb
T18429	54-58	P03230	denotes	LMP1
T18430	207-211	P03230	denotes	LMP1
T20533	5073-5077	P15391	denotes	CD19
T21194	6717-6719	P0A7Z4	denotes	pH

test2

Id	Subject	Object	Predicate	Lexical cue
T18281	54-58	Protein	denotes	LMP1
T18282	207-211	Protein	denotes	LMP1
T18596	1205-1209	Protein	denotes	CD19
T18597	1560-1564	Protein	denotes	CD19
T18598	1664-1667	Protein	denotes	IL4
T18599	1710-1714	Protein	denotes	IL10
T18600	1752-1756	Protein	denotes	IL10
T19030	2214-2217	Protein	denotes	IL4
T19031	2301-2304	Protein	denotes	IL4
T19032	2629-2632	Protein	denotes	IL4
T19033	2654-2657	Protein	denotes	IL4
T19630	3564-3568	Protein	denotes	LMP1
T19631	3613-3617	Protein	denotes	LMP1
T19632	3762-3766	Protein	denotes	LMP1
T19633	4144-4151	Phosphorylation	denotes	Phospho
T19634	4152-4157	Protein	denotes	Stat3
T20328	5073-5077	Protein	denotes	CD19
T20329	5821-5828	Protein	denotes	Rnase A
T20330	5833-5835	Protein	denotes	T1
T20765	6313-6331	Protein	denotes	Taq DNA polymerase
T21025	7410-7420	Protein	denotes	peroxidase
T21406	7887-7891	Protein	denotes	CD19
T21407	7937-7941	Protein	denotes	LMP1
T21408	7986-7988	Protein	denotes	Rb
T21409	8009-8013	Protein	denotes	Cdk2
T21410	8096-8099	Protein	denotes	CD5
T21411	8156-8160	Protein	denotes	LMP1
T21412	8235-8239	Protein	denotes	pAkt
T21413	8255-8261	Protein	denotes	pGSK3α
T21414	8262-8263	Protein	denotes	β
T21415	8280-8286	Protein	denotes	pStat3
T21416	8302-8308	Protein	denotes	pStat6
T21417	8324-8329	Protein	denotes	pmTOR
T21418	8346-8351	Protein	denotes	Stat6
T21419	8358-8361	Protein	denotes	Akt
T21420	8368-8373	Protein	denotes	FoxO1
T21421	8384-8387	Protein	denotes	p27
T21422	8436-8441	Protein	denotes	Stat3
T21423	8456-8460	Protein	denotes	IκBα
T21424	8483-8490	Protein	denotes	β actin
T21425	8561-8565	Protein	denotes	GSK3
T21426	8620-8623	Protein	denotes	pRb
R15242	T19634	T19633	themeOf	Stat3,Phospho

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