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2 LMP1 Promotes B-1a Lymphomas That Can Escape Allelic Exclusion\n(A) Flow cytometry analysis of splenocytes from a WT or LMP1 transgenic lymphoma for the pan–B cell (CD19), B-1a cell (CD5), and Ig heavy chain (IgM and IgD) and light chain (κ and λ) markers. Shown are the results from WT lymphoma 1 and LMP1 transgenic lymphoma 4. This analysis was repeated on four other LMP1 transgenic lymphomas (1, 2, 3, and 6) showing a similar B-1a phenotype, of which lymphomas 2 and 4 were also doubly positive for κ and λ light chains.\n(B) Flow cytometry analysis of WT or LMP1 transgenic splenocytes for B-1a (CD19+CD5+) and B-1b or B2 subsets (CD19+CD5−). Percentages of B-1a and B-1b or B2 subsets are shown in each quadrant. This analysis was repeated on three other WT and two other LMP1 transgenic mice with similar results.\n(C) Immunoblot analysis for κ and λ light chains of B cells (CD19+) purified from WT and LMP1 transgenic mice. Actin was used as a loading control. "}

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2 LMP1 Promotes B-1a Lymphomas That Can Escape Allelic Exclusion\n(A) Flow cytometry analysis of splenocytes from a WT or LMP1 transgenic lymphoma for the pan–B cell (CD19), B-1a cell (CD5), and Ig heavy chain (IgM and IgD) and light chain (κ and λ) markers. Shown are the results from WT lymphoma 1 and LMP1 transgenic lymphoma 4. This analysis was repeated on four other LMP1 transgenic lymphomas (1, 2, 3, and 6) showing a similar B-1a phenotype, of which lymphomas 2 and 4 were also doubly positive for κ and λ light chains.\n(B) Flow cytometry analysis of WT or LMP1 transgenic splenocytes for B-1a (CD19+CD5+) and B-1b or B2 subsets (CD19+CD5−). Percentages of B-1a and B-1b or B2 subsets are shown in each quadrant. This analysis was repeated on three other WT and two other LMP1 transgenic mice with similar results.\n(C) Immunoblot analysis for κ and λ light chains of B cells (CD19+) purified from WT and LMP1 transgenic mice. Actin was used as a loading control. "}

{"project":"GO-CC","denotations":[{"id":"T22555","span":{"begin":168,"end":172},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T22556","span":{"begin":186,"end":190},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T22557","span":{"begin":885,"end":890},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Figure 2 LMP1 Promotes B-1a Lymphomas That Can Escape Allelic Exclusion\n(A) Flow cytometry analysis of splenocytes from a WT or LMP1 transgenic lymphoma for the pan–B cell (CD19), B-1a cell (CD5), and Ig heavy chain (IgM and IgD) and light chain (κ and λ) markers. Shown are the results from WT lymphoma 1 and LMP1 transgenic lymphoma 4. This analysis was repeated on four other LMP1 transgenic lymphomas (1, 2, 3, and 6) showing a similar B-1a phenotype, of which lymphomas 2 and 4 were also doubly positive for κ and λ light chains.\n(B) Flow cytometry analysis of WT or LMP1 transgenic splenocytes for B-1a (CD19+CD5+) and B-1b or B2 subsets (CD19+CD5−). Percentages of B-1a and B-1b or B2 subsets are shown in each quadrant. This analysis was repeated on three other WT and two other LMP1 transgenic mice with similar results.\n(C) Immunoblot analysis for κ and λ light chains of B cells (CD19+) purified from WT and LMP1 transgenic mice. Actin was used as a loading control. "}

{"project":"sentences","denotations":[{"id":"T22113","span":{"begin":10,"end":72},"obj":"Sentence"},{"id":"T22114","span":{"begin":73,"end":265},"obj":"Sentence"},{"id":"T22115","span":{"begin":266,"end":338},"obj":"Sentence"},{"id":"T22116","span":{"begin":339,"end":535},"obj":"Sentence"},{"id":"T22117","span":{"begin":536,"end":657},"obj":"Sentence"},{"id":"T22118","span":{"begin":658,"end":728},"obj":"Sentence"},{"id":"T22119","span":{"begin":729,"end":830},"obj":"Sentence"},{"id":"T22120","span":{"begin":831,"end":941},"obj":"Sentence"},{"id":"T22121","span":{"begin":942,"end":978},"obj":"Sentence"},{"id":"T73","span":{"begin":0,"end":72},"obj":"Sentence"},{"id":"T74","span":{"begin":73,"end":265},"obj":"Sentence"},{"id":"T75","span":{"begin":266,"end":338},"obj":"Sentence"},{"id":"T76","span":{"begin":339,"end":535},"obj":"Sentence"},{"id":"T77","span":{"begin":536,"end":657},"obj":"Sentence"},{"id":"T78","span":{"begin":658,"end":728},"obj":"Sentence"},{"id":"T79","span":{"begin":729,"end":830},"obj":"Sentence"},{"id":"T80","span":{"begin":831,"end":941},"obj":"Sentence"},{"id":"T81","span":{"begin":942,"end":978},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Figure 2 LMP1 Promotes B-1a Lymphomas That Can Escape Allelic Exclusion\n(A) Flow cytometry analysis of splenocytes from a WT or LMP1 transgenic lymphoma for the pan–B cell (CD19), B-1a cell (CD5), and Ig heavy chain (IgM and IgD) and light chain (κ and λ) markers. Shown are the results from WT lymphoma 1 and LMP1 transgenic lymphoma 4. This analysis was repeated on four other LMP1 transgenic lymphomas (1, 2, 3, and 6) showing a similar B-1a phenotype, of which lymphomas 2 and 4 were also doubly positive for κ and λ light chains.\n(B) Flow cytometry analysis of WT or LMP1 transgenic splenocytes for B-1a (CD19+CD5+) and B-1b or B2 subsets (CD19+CD5−). Percentages of B-1a and B-1b or B2 subsets are shown in each quadrant. This analysis was repeated on three other WT and two other LMP1 transgenic mice with similar results.\n(C) Immunoblot analysis for κ and λ light chains of B cells (CD19+) purified from WT and LMP1 transgenic mice. Actin was used as a loading control. "}

{"project":"events-check-again","denotations":[{"id":"T22609","span":{"begin":10,"end":14},"obj":"Protein"},{"id":"T22610","span":{"begin":129,"end":133},"obj":"Protein"},{"id":"T22611","span":{"begin":174,"end":178},"obj":"Protein"},{"id":"T22612","span":{"begin":192,"end":195},"obj":"Protein"},{"id":"T22613","span":{"begin":311,"end":315},"obj":"Protein"},{"id":"T22614","span":{"begin":380,"end":384},"obj":"Protein"},{"id":"T22615","span":{"begin":573,"end":577},"obj":"Protein"},{"id":"T22616","span":{"begin":611,"end":615},"obj":"Protein"},{"id":"T22617","span":{"begin":616,"end":619},"obj":"Protein"},{"id":"T22618","span":{"begin":646,"end":650},"obj":"Protein"},{"id":"T22619","span":{"begin":651,"end":654},"obj":"Protein"},{"id":"T22620","span":{"begin":788,"end":792},"obj":"Protein"},{"id":"T22621","span":{"begin":892,"end":896},"obj":"Protein"},{"id":"T22622","span":{"begin":920,"end":924},"obj":"Protein"}],"text":"Figure 2 LMP1 Promotes B-1a Lymphomas That Can Escape Allelic Exclusion\n(A) Flow cytometry analysis of splenocytes from a WT or LMP1 transgenic lymphoma for the pan–B cell (CD19), B-1a cell (CD5), and Ig heavy chain (IgM and IgD) and light chain (κ and λ) markers. Shown are the results from WT lymphoma 1 and LMP1 transgenic lymphoma 4. This analysis was repeated on four other LMP1 transgenic lymphomas (1, 2, 3, and 6) showing a similar B-1a phenotype, of which lymphomas 2 and 4 were also doubly positive for κ and λ light chains.\n(B) Flow cytometry analysis of WT or LMP1 transgenic splenocytes for B-1a (CD19+CD5+) and B-1b or B2 subsets (CD19+CD5−). Percentages of B-1a and B-1b or B2 subsets are shown in each quadrant. This analysis was repeated on three other WT and two other LMP1 transgenic mice with similar results.\n(C) Immunoblot analysis for κ and λ light chains of B cells (CD19+) purified from WT and LMP1 transgenic mice. Actin was used as a loading control. "}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T22586","span":{"begin":10,"end":14},"obj":"Protein"},{"id":"T22587","span":{"begin":48,"end":72},"obj":"Protein"},{"id":"T22588","span":{"begin":123,"end":125},"obj":"Protein"},{"id":"T22589","span":{"begin":129,"end":133},"obj":"Protein"},{"id":"T22590","span":{"begin":202,"end":216},"obj":"Protein"},{"id":"T22591","span":{"begin":218,"end":221},"obj":"Protein"},{"id":"T22592","span":{"begin":226,"end":229},"obj":"Protein"},{"id":"T22593","span":{"begin":293,"end":306},"obj":"Protein"},{"id":"T22594","span":{"begin":311,"end":315},"obj":"Protein"},{"id":"T22595","span":{"begin":567,"end":569},"obj":"Protein"},{"id":"T22596","span":{"begin":573,"end":577},"obj":"Protein"},{"id":"T22597","span":{"begin":605,"end":609},"obj":"Protein"},{"id":"T22598","span":{"begin":611,"end":615},"obj":"Protein"},{"id":"T22599","span":{"begin":616,"end":619},"obj":"Protein"},{"id":"T22600","span":{"begin":626,"end":630},"obj":"Protein"},{"id":"T22601","span":{"begin":634,"end":644},"obj":"Protein"},{"id":"T22602","span":{"begin":646,"end":650},"obj":"Protein"},{"id":"T22603","span":{"begin":651,"end":654},"obj":"Protein"},{"id":"T22604","span":{"begin":788,"end":792},"obj":"Protein"},{"id":"T22605","span":{"begin":892,"end":896},"obj":"Protein"},{"id":"T22606","span":{"begin":913,"end":915},"obj":"Protein"},{"id":"T22607","span":{"begin":920,"end":924},"obj":"Protein"},{"id":"T22608","span":{"begin":942,"end":947},"obj":"Protein"}],"text":"Figure 2 LMP1 Promotes B-1a Lymphomas That Can Escape Allelic Exclusion\n(A) Flow cytometry analysis of splenocytes from a WT or LMP1 transgenic lymphoma for the pan–B cell (CD19), B-1a cell (CD5), and Ig heavy chain (IgM and IgD) and light chain (κ and λ) markers. Shown are the results from WT lymphoma 1 and LMP1 transgenic lymphoma 4. This analysis was repeated on four other LMP1 transgenic lymphomas (1, 2, 3, and 6) showing a similar B-1a phenotype, of which lymphomas 2 and 4 were also doubly positive for κ and λ light chains.\n(B) Flow cytometry analysis of WT or LMP1 transgenic splenocytes for B-1a (CD19+CD5+) and B-1b or B2 subsets (CD19+CD5−). Percentages of B-1a and B-1b or B2 subsets are shown in each quadrant. This analysis was repeated on three other WT and two other LMP1 transgenic mice with similar results.\n(C) Immunoblot analysis for κ and λ light chains of B cells (CD19+) purified from WT and LMP1 transgenic mice. Actin was used as a loading control. "}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T22099","span":{"begin":10,"end":14},"obj":"Protein"},{"id":"T22100","span":{"begin":129,"end":133},"obj":"Protein"},{"id":"T22101","span":{"begin":174,"end":178},"obj":"Protein"},{"id":"T22102","span":{"begin":192,"end":195},"obj":"Protein"},{"id":"T22103","span":{"begin":311,"end":315},"obj":"Protein"},{"id":"T22104","span":{"begin":380,"end":384},"obj":"Protein"},{"id":"T22105","span":{"begin":573,"end":577},"obj":"Protein"},{"id":"T22106","span":{"begin":611,"end":615},"obj":"Protein"},{"id":"T22107","span":{"begin":616,"end":619},"obj":"Protein"},{"id":"T22108","span":{"begin":646,"end":650},"obj":"Protein"},{"id":"T22109","span":{"begin":651,"end":654},"obj":"Protein"},{"id":"T22110","span":{"begin":788,"end":792},"obj":"Protein"},{"id":"T22111","span":{"begin":892,"end":896},"obj":"Protein"},{"id":"T22112","span":{"begin":920,"end":924},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Figure 2 LMP1 Promotes B-1a Lymphomas That Can Escape Allelic Exclusion\n(A) Flow cytometry analysis of splenocytes from a WT or LMP1 transgenic lymphoma for the pan–B cell (CD19), B-1a cell (CD5), and Ig heavy chain (IgM and IgD) and light chain (κ and λ) markers. Shown are the results from WT lymphoma 1 and LMP1 transgenic lymphoma 4. This analysis was repeated on four other LMP1 transgenic lymphomas (1, 2, 3, and 6) showing a similar B-1a phenotype, of which lymphomas 2 and 4 were also doubly positive for κ and λ light chains.\n(B) Flow cytometry analysis of WT or LMP1 transgenic splenocytes for B-1a (CD19+CD5+) and B-1b or B2 subsets (CD19+CD5−). Percentages of B-1a and B-1b or B2 subsets are shown in each quadrant. This analysis was repeated on three other WT and two other LMP1 transgenic mice with similar results.\n(C) Immunoblot analysis for κ and λ light chains of B cells (CD19+) purified from WT and LMP1 transgenic mice. Actin was used as a loading control. "}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T22319","span":{"begin":10,"end":14},"obj":"P03230"},{"id":"T22320","span":{"begin":129,"end":133},"obj":"P03230"},{"id":"T22321","span":{"begin":174,"end":178},"obj":"P15391"},{"id":"T22322","span":{"begin":192,"end":195},"obj":"P06127"},{"id":"T22323","span":{"begin":311,"end":315},"obj":"P03230"},{"id":"T22324","span":{"begin":380,"end":384},"obj":"P03230"},{"id":"T22325","span":{"begin":573,"end":577},"obj":"P03230"},{"id":"T22326","span":{"begin":611,"end":615},"obj":"P15391"},{"id":"T22327","span":{"begin":616,"end":619},"obj":"P06127"},{"id":"T22328","span":{"begin":646,"end":650},"obj":"P15391"},{"id":"T22329","span":{"begin":651,"end":654},"obj":"P06127"},{"id":"T22330","span":{"begin":788,"end":792},"obj":"P03230"},{"id":"T22331","span":{"begin":892,"end":896},"obj":"P15391"},{"id":"T22332","span":{"begin":920,"end":924},"obj":"P03230"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Figure 2 LMP1 Promotes B-1a Lymphomas That Can Escape Allelic Exclusion\n(A) Flow cytometry analysis of splenocytes from a WT or LMP1 transgenic lymphoma for the pan–B cell (CD19), B-1a cell (CD5), and Ig heavy chain (IgM and IgD) and light chain (κ and λ) markers. Shown are the results from WT lymphoma 1 and LMP1 transgenic lymphoma 4. This analysis was repeated on four other LMP1 transgenic lymphomas (1, 2, 3, and 6) showing a similar B-1a phenotype, of which lymphomas 2 and 4 were also doubly positive for κ and λ light chains.\n(B) Flow cytometry analysis of WT or LMP1 transgenic splenocytes for B-1a (CD19+CD5+) and B-1b or B2 subsets (CD19+CD5−). Percentages of B-1a and B-1b or B2 subsets are shown in each quadrant. This analysis was repeated on three other WT and two other LMP1 transgenic mice with similar results.\n(C) Immunoblot analysis for κ and λ light chains of B cells (CD19+) purified from WT and LMP1 transgenic mice. Actin was used as a loading control. "}

{"project":"test2","denotations":[{"id":"T22085","span":{"begin":10,"end":14},"obj":"Protein"},{"id":"T22086","span":{"begin":129,"end":133},"obj":"Protein"},{"id":"T22087","span":{"begin":174,"end":178},"obj":"Protein"},{"id":"T22088","span":{"begin":192,"end":195},"obj":"Protein"},{"id":"T22089","span":{"begin":311,"end":315},"obj":"Protein"},{"id":"T22090","span":{"begin":380,"end":384},"obj":"Protein"},{"id":"T22091","span":{"begin":573,"end":577},"obj":"Protein"},{"id":"T22092","span":{"begin":611,"end":615},"obj":"Protein"},{"id":"T22093","span":{"begin":616,"end":619},"obj":"Protein"},{"id":"T22094","span":{"begin":646,"end":650},"obj":"Protein"},{"id":"T22095","span":{"begin":651,"end":654},"obj":"Protein"},{"id":"T22096","span":{"begin":788,"end":792},"obj":"Protein"},{"id":"T22097","span":{"begin":892,"end":896},"obj":"Protein"},{"id":"T22098","span":{"begin":920,"end":924},"obj":"Protein"}],"text":"Figure 2 LMP1 Promotes B-1a Lymphomas That Can Escape Allelic Exclusion\n(A) Flow cytometry analysis of splenocytes from a WT or LMP1 transgenic lymphoma for the pan–B cell (CD19), B-1a cell (CD5), and Ig heavy chain (IgM and IgD) and light chain (κ and λ) markers. Shown are the results from WT lymphoma 1 and LMP1 transgenic lymphoma 4. This analysis was repeated on four other LMP1 transgenic lymphomas (1, 2, 3, and 6) showing a similar B-1a phenotype, of which lymphomas 2 and 4 were also doubly positive for κ and λ light chains.\n(B) Flow cytometry analysis of WT or LMP1 transgenic splenocytes for B-1a (CD19+CD5+) and B-1b or B2 subsets (CD19+CD5−). Percentages of B-1a and B-1b or B2 subsets are shown in each quadrant. This analysis was repeated on three other WT and two other LMP1 transgenic mice with similar results.\n(C) Immunoblot analysis for κ and λ light chains of B cells (CD19+) purified from WT and LMP1 transgenic mice. Actin was used as a loading control. "}