PMC:2063610 / 10704-11629 JSONTXT

{"project":"CellFinder","denotations":[{"id":"T652","span":{"begin":587,"end":591},"obj":"GeneProtein"},{"id":"T653","span":{"begin":398,"end":402},"obj":"GeneProtein"},{"id":"T654","span":{"begin":329,"end":333},"obj":"GeneProtein"},{"id":"T655","span":{"begin":467,"end":473},"obj":"CellComponent"},{"id":"T656","span":{"begin":579,"end":585},"obj":"GeneProtein"},{"id":"T657","span":{"begin":65,"end":80},"obj":"CellType"},{"id":"T658","span":{"begin":306,"end":312},"obj":"GeneProtein"},{"id":"T659","span":{"begin":536,"end":545},"obj":"CellType"},{"id":"T660","span":{"begin":92,"end":107},"obj":"CellType"},{"id":"T661","span":{"begin":536,"end":545},"obj":"Anatomy"}],"text":"Fig. 1 The age of sera determined the regenerative potential of satellite cells. (A) Young satellite cells were cultured either in 5% or 10% young (Young), 10% old (Old), or in a 5%+ 5% mouse sera combination (young + old). Cells were analyzed by immunofluorescence microscopy, using anti-BrdU (red), antidesmin (green) or anti-Myf5 antibodies (green, small panels). Similar results are shown for Pax7 immunodetection (Supplementary Fig. S1). Hoechst (blue) labeled nuclei. (B) Three independent experiments were quantified [300 young myofibers per experiment] as percentage of desmin+/Myf5+/BrdU+ de novo generated cells for each age and culture condition. On average, two to three fewer cells were generated when cultured in the presence of old. Shown are identical microscope fields at ×40 magnification. At least three independent experiments produced similar results. (*) indicates P≤ 0.001 as compared to young sera. "}