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PMC:194730 / 7180-11476 JSONTXT

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Id Subject Object Predicate Lexical cue
T2443 0-7 NN denotes Annexin
T2444 8-10 NN denotes A7
T2445 11-21 NN denotes expression
T2446 22-24 IN denotes in
T2447 25-28 JJ denotes red
T2448 35-40 NNS denotes cells
T2449 29-34 NN denotes blood
T2450 40-42 , denotes ,
T2451 42-45 JJ denotes red
T2452 52-56 NN denotes cell
T2453 46-51 NN denotes blood
T2454 57-64 VBN denotes derived
T2455 65-76 NNS denotes exovesicles
T2456 77-80 CC denotes and
T2457 81-83 IN denotes in
T2458 84-93 NNS denotes platelets
T2459 93-334 sentence denotes Salzer et al. recently reported the presence of the 47 kDa isoform of annexin A7 and its partner sorcin in micro- and nanovesicles derived from red blood cells where they are located in the lumen and are also enriched in membrane rafts [7].
T2460 94-100 NNP denotes Salzer
T2461 117-125 VBD denotes reported
T2462 101-103 FW denotes et
T2463 104-107 FW denotes al.
T2464 108-116 RB denotes recently
T2465 126-129 DT denotes the
T2466 130-138 NN denotes presence
T2467 139-141 IN denotes of
T2468 142-145 DT denotes the
T2469 153-160 NN denotes isoform
T2470 146-148 CD denotes 47
T2471 149-152 NN denotes kDa
T2472 161-163 IN denotes of
T2473 164-171 NN denotes annexin
T2474 172-174 NN denotes A7
T2475 175-178 CC denotes and
T2476 179-182 PRP$ denotes its
T2477 183-190 NN denotes partner
T2478 191-197 NN denotes sorcin
T2479 198-200 IN denotes in
T2480 201-206 NN denotes micro
T2481 206-207 HYPH denotes -
T2482 208-211 CC denotes and
T2483 212-224 NNS denotes nanovesicles
T2484 225-232 VBN denotes derived
T2485 233-237 IN denotes from
T2486 238-241 JJ denotes red
T2487 248-253 NNS denotes cells
T2488 242-247 NN denotes blood
T2489 254-259 WRB denotes where
T2490 269-276 VBN denotes located
T2491 260-264 PRP denotes they
T2492 265-268 VBP denotes are
T2493 277-279 IN denotes in
T2494 280-283 DT denotes the
T2495 284-289 NN denotes lumen
T2496 290-293 CC denotes and
T2497 294-297 VBP denotes are
T2498 303-311 VBN denotes enriched
T2499 298-302 RB denotes also
T2500 312-314 IN denotes in
T2501 315-323 NN denotes membrane
T2502 324-329 NNS denotes rafts
T2503 330-331 -LRB- denotes [
T2504 331-332 CD denotes 7
T2505 332-333 -RRB- denotes ]
T2506 333-334 . denotes .
T2507 334-428 sentence denotes We have now extended these findings and show here that the 51 kDa isoform is present as well.
T2508 335-337 PRP denotes We
T2509 347-355 VBN denotes extended
T2510 338-342 VBP denotes have
T2511 343-346 RB denotes now
T2512 356-361 DT denotes these
T2513 362-370 NNS denotes findings
T2514 371-374 CC denotes and
T2515 375-379 VBP denotes show
T2516 380-384 RB denotes here
T2517 385-389 IN denotes that
T2518 409-411 VBZ denotes is
T2519 390-393 DT denotes the
T2520 401-408 NN denotes isoform
T2521 394-396 CD denotes 51
T2522 397-400 NN denotes kDa
T2523 412-419 JJ denotes present
T2524 420-422 RB denotes as
T2525 423-427 RB denotes well
T2526 427-428 . denotes .
T2527 428-539 sentence denotes In western blots of human red blood cells the 47 and 51 kDa isoforms were detected using mAb 203–217 (Fig. 1).
T2528 429-431 IN denotes In
T2529 503-511 VBN denotes detected
T2530 432-439 JJ denotes western
T2531 440-445 NNS denotes blots
T2532 446-448 IN denotes of
T2533 449-454 JJ denotes human
T2534 465-470 NNS denotes cells
T2535 455-458 JJ denotes red
T2536 459-464 NN denotes blood
T2537 471-474 DT denotes the
T2538 489-497 NNS denotes isoforms
T2539 475-477 CD denotes 47
T2540 485-488 NN denotes kDa
T2541 478-481 CC denotes and
T2542 482-484 CD denotes 51
T2543 498-502 VBD denotes were
T2544 512-517 VBG denotes using
T2545 518-521 NN denotes mAb
T2546 522-529 CD denotes 203–217
T2547 530-531 -LRB- denotes (
T2548 531-535 NN denotes Fig.
T2549 536-537 CD denotes 1
T2550 537-538 -RRB- denotes )
T2551 538-539 . denotes .
T2552 539-686 sentence denotes The 47 kDa isoform was detected in silver stained gels and its identity with annexin A7 confirmed by peptide mass fingerprinting (data not shown).
T2553 540-543 DT denotes The
T2606 1180-1185 IN denotes along
T2607 1186-1190 IN denotes with
T2608 1191-1195 NN denotes raft
T2609 1196-1203 NNS denotes domains
T2610 1203-1205 , denotes ,
T2611 1205-1212 NN denotes annexin
T2612 1213-1215 NN denotes A7
T2613 1222-1223 DT denotes a
T2614 1224-1228 NN denotes role
T2615 1229-1231 IN denotes in
T2616 1232-1240 NN denotes membrane
T2617 1241-1253 NN denotes organization
T2618 1254-1257 CC denotes and
T2619 1258-1261 DT denotes the
T2620 1275-1282 NN denotes process
T2621 1262-1274 NN denotes vesiculation
T2622 1282-1283 . denotes .
T2623 1283-1418 sentence denotes To examine this, we analysed the ability of red blood cells derived from the annexin A7 knock out mice (anxA7-/-) to form exovesicles.
T2624 1284-1286 TO denotes To
T2625 1287-1294 VB denotes examine
T2626 1304-1312 VBD denotes analysed
T2627 1295-1299 DT denotes this
T2628 1299-1301 , denotes ,
T2629 1301-1303 PRP denotes we
T2630 1313-1316 DT denotes the
T2631 1317-1324 NN denotes ability
T2632 1325-1327 IN denotes of
T2633 1328-1331 JJ denotes red
T2634 1338-1343 NNS denotes cells
T2635 1332-1337 NN denotes blood
T2636 1344-1351 VBN denotes derived
T2637 1352-1356 IN denotes from
T2638 1357-1360 DT denotes the
T2639 1382-1386 NNS denotes mice
T2640 1361-1368 NN denotes annexin
T2641 1369-1371 NN denotes A7
T2642 1372-1377 NN denotes knock
T2643 1378-1381 NN denotes out
T2644 1387-1388 -LRB- denotes (
T2645 1388-1393 NN denotes anxA7
T2646 1393-1394 SYM denotes -
T2647 1394-1395 HYPH denotes /
T2648 1395-1396 SYM denotes -
T2649 1396-1397 -RRB- denotes )
T2650 1398-1400 TO denotes to
T2651 1401-1405 VB denotes form
T2652 1406-1417 NNS denotes exovesicles
T2653 1417-1418 . denotes .
T2654 1418-1554 sentence denotes Because of better accessibility and larger available amounts that led to clear results, we included our data obtained with human blood.
T2655 1419-1426 IN denotes Because
T2656 1510-1518 VBD denotes included
T2657 1427-1429 IN denotes of
T2658 1430-1436 JJR denotes better
T2659 1437-1450 NN denotes accessibility
T2660 1451-1454 CC denotes and
T2661 1455-1461 JJR denotes larger
T2662 1472-1479 NNS denotes amounts
T2663 1462-1471 JJ denotes available
T2664 1480-1484 WDT denotes that
T2665 1485-1488 VBD denotes led
T2666 1489-1491 IN denotes to
T2667 1492-1497 JJ denotes clear
T2668 1498-1505 NNS denotes results
T2669 1505-1507 , denotes ,
T2670 1507-1509 PRP denotes we
T2671 1519-1522 PRP$ denotes our
T2672 1523-1527 NNS denotes data
T2673 1528-1536 VBN denotes obtained
T2674 1537-1541 IN denotes with
T2675 1542-1547 JJ denotes human
T2676 1548-1553 NN denotes blood
T2677 1553-1554 . denotes .
T2678 1554-1771 sentence denotes Independent of the presence of annexin A7 both types of exovesicles, micro- and nanovesicles, were released after Ca2+/ionophore treatment as determined by acetylcholine esterase activity and microscopic examination.
T2679 1555-1566 JJ denotes Independent
T2680 1654-1662 VBN denotes released
T2681 1567-1569 IN denotes of
T2682 1570-1573 DT denotes the
T2683 1574-1582 NN denotes presence
T2684 1583-1585 IN denotes of
T2685 1586-1593 NN denotes annexin
T2686 1594-1596 NN denotes A7
T2687 1597-1601 DT denotes both
T2688 1602-1607 NNS denotes types
T2689 1608-1610 IN denotes of
T2690 1611-1622 NNS denotes exovesicles
T2691 1622-1624 , denotes ,
T2692 1624-1629 AFX denotes micro
T2693 1635-1647 NNS denotes nanovesicles
T2694 1629-1630 HYPH denotes -
T2695 1631-1634 CC denotes and
T2696 1647-1649 , denotes ,
T2697 1649-1653 VBD denotes were
T2698 1663-1668 IN denotes after
T2699 1669-1673 NN denotes Ca2+
T2700 1674-1683 NN denotes ionophore
T2701 1673-1674 HYPH denotes /
T2702 1684-1693 NN denotes treatment
T2703 1694-1696 IN denotes as
T2704 1697-1707 VBN denotes determined
T2705 1708-1710 IN denotes by
T2706 1711-1724 NN denotes acetylcholine
T2707 1725-1733 NN denotes esterase
T2708 1734-1742 NN denotes activity
T2709 1743-1746 CC denotes and
T2710 1747-1758 JJ denotes microscopic
T2711 1759-1770 NN denotes examination
T2712 1770-1771 . denotes .
T2713 1771-1867 sentence denotes Annexin A7 is present in both vesicle types where it is more enriched in nanovesicles (Fig. 2).
T2714 1772-1779 NN denotes Annexin
T2715 1780-1782 NN denotes A7
T2716 1783-1785 VBZ denotes is
T2717 1786-1793 JJ denotes present
T2718 1794-1796 IN denotes in
T2719 1797-1801 CC denotes both
T2720 1810-1815 NNS denotes types
T2721 1802-1809 NN denotes vesicle
T2722 1816-1821 WRB denotes where
T2723 1825-1827 VBZ denotes is
T2724 1822-1824 PRP denotes it
T2725 1828-1832 RBR denotes more
T2726 1833-1841 JJ denotes enriched
T2727 1842-1844 IN denotes in
T2728 1845-1857 NNS denotes nanovesicles
T2729 1858-1859 -LRB- denotes (
T2730 1859-1863 NN denotes Fig.
T2731 1864-1865 CD denotes 2
T2732 1865-1866 -RRB- denotes )
T2733 1866-1867 . denotes .
T2734 1867-1922 sentence denotes The 51 kDa isoform is only detectable in nanovesicles.
T2735 1868-1871 DT denotes The
T2736 1879-1886 NN denotes isoform
T2737 1872-1874 CD denotes 51
T2738 1875-1878 NN denotes kDa
T2739 1887-1889 VBZ denotes is
T2740 1890-1894 RB denotes only
T2741 1895-1905 JJ denotes detectable
T2742 1906-1908 IN denotes in
T2743 1909-1921 NNS denotes nanovesicles
T2744 1921-1922 . denotes .
T2745 1922-2136 sentence denotes The quantity of both vesicle types did not differ between wild type and knock out red blood cell vesicles as determined by the AChE-values (A405 nanovesicles: ~0.18; A405 microvesicles: ~0.9; both, for wt and ko).
T2746 1923-1926 DT denotes The
T2747 1927-1935 NN denotes quantity
T2748 1966-1972 VB denotes differ
T2749 1936-1938 IN denotes of
T2750 1939-1943 DT denotes both
T2751 1952-1957 NNS denotes types
T2752 1944-1951 NN denotes vesicle
T2753 1958-1961 VBD denotes did
T2754 1962-1965 RB denotes not
T2755 1973-1980 IN denotes between
T2756 1981-1985 JJ denotes wild
T2757 1986-1990 NN denotes type
T2758 2020-2028 NNS denotes vesicles
T2759 1991-1994 CC denotes and
T2760 1995-2000 NN denotes knock
T2761 2001-2004 NN denotes out
T2762 2005-2008 JJ denotes red
T2763 2015-2019 NN denotes cell
T2764 2009-2014 NN denotes blood
T2765 2029-2031 IN denotes as
T2766 2032-2042 VBN denotes determined
T2767 2043-2045 IN denotes by
T2768 2046-2049 DT denotes the
T2769 2055-2061 NNS denotes values
T2770 2050-2054 NN denotes AChE
T2771 2054-2055 HYPH denotes -
T2772 2062-2063 -LRB- denotes (
T2773 2115-2119 DT denotes both
T2774 2063-2067 NN denotes A405
T2775 2068-2080 NNS denotes nanovesicles
T2776 2083-2087 CD denotes 0.18
T2777 2080-2082 : denotes :
T2778 2082-2083 SYM denotes ~
T2779 2087-2088 : denotes ;
T2780 2089-2093 NN denotes A405
T2781 2094-2107 NNS denotes microvesicles
T2782 2110-2113 CD denotes 0.9
T2783 2107-2109 : denotes :
T2784 2109-2110 SYM denotes ~
T2785 2113-2114 : denotes ;
T2786 2119-2121 , denotes ,
T2787 2121-2124 IN denotes for
T2788 2125-2127 NN denotes wt
T2789 2128-2131 CC denotes and
T2790 2132-2134 NN denotes ko
T2791 2134-2135 -RRB- denotes )
T2832 2320-2323 DT denotes the
T2833 2324-2332 NN denotes presence
T2834 2333-2335 IN denotes of
T2835 2336-2342 NN denotes sorcin
T2836 2343-2345 IN denotes in
T2837 2346-2350 JJ denotes wild
T2838 2351-2355 NN denotes type
T2839 2367-2375 NNS denotes vesicles
T2840 2356-2359 CC denotes and
T2841 2360-2366 JJ denotes mutant
T2842 2376-2378 PRP denotes we
T2843 2385-2389 IN denotes that
T2844 2413-2420 VBN denotes reduced
T2845 2390-2393 DT denotes the
T2846 2401-2407 NNS denotes levels
T2847 2394-2400 NN denotes sorcin
T2848 2408-2412 VBD denotes were
T2849 2421-2423 IN denotes in
T2850 2424-2427 DT denotes the
T2851 2435-2447 NNS denotes nanovesicles
T2852 2428-2434 JJ denotes mutant
T2853 2448-2449 -LRB- denotes (
T2854 2449-2453 NN denotes Fig.
T2855 2454-2455 CD denotes 2
T2856 2455-2456 -RRB- denotes )
T2857 2456-2457 . denotes .
T2880 3208-3216 NNS denotes isoforms
T2881 3217-3221 VBD denotes were
T2882 3222-3229 JJ denotes present
T2883 3230-3232 IN denotes in
T2884 3233-3236 DT denotes the
T2885 3245-3253 NN denotes fraction
T2886 3237-3244 JJ denotes soluble
T2887 3254-3256 RB denotes as
T2888 3262-3264 IN denotes as
T2889 3257-3261 RB denotes well
T2890 3265-3267 IN denotes in
T2891 3268-3271 DT denotes the
T2892 3281-3290 NNS denotes fractions
T2893 3272-3280 NN denotes gradient
T2894 3291-3296 WRB denotes where
T2895 3306-3313 VBN denotes assumed
T2896 3297-3301 PRP denotes they
T2897 3302-3305 VBP denotes are
T2898 3314-3316 TO denotes to
T2899 3320-3330 VBN denotes associated
T2900 3317-3319 VB denotes be
T2901 3331-3335 IN denotes with
T2902 3336-3345 NNS denotes membranes
T2903 3345-3346 . denotes .
T2904 3346-3442 sentence denotes These membranes are exclusively plasma membranes as red blood cells are free of any organelles.
T2905 3347-3352 DT denotes These
T2906 3353-3362 NNS denotes membranes
T2907 3363-3366 VBP denotes are
T2908 3367-3378 RB denotes exclusively
T2909 3379-3385 NN denotes plasma
T2910 3386-3395 NNS denotes membranes
T2911 3396-3398 IN denotes as
T2912 3415-3418 VBP denotes are
T2913 3399-3402 JJ denotes red
T2914 3409-3414 NNS denotes cells
T2915 3403-3408 NN denotes blood
T2916 3419-3423 JJ denotes free
T2917 3424-3426 IN denotes of
T2918 3427-3430 DT denotes any
T2919 3431-3441 NNS denotes organelles
T2920 3441-3442 . denotes .
T2921 3442-3512 sentence denotes The distribution was however not homogeneous throughout the gradient.
T2922 3443-3446 DT denotes The
T2923 3447-3459 NN denotes distribution
T2924 3460-3463 VBD denotes was
T2925 3464-3471 RB denotes however
T2926 3472-3475 RB denotes not
T2927 3476-3487 JJ denotes homogeneous
T2928 3488-3498 IN denotes throughout
T2929 3499-3502 DT denotes the
T2930 3503-3511 NN denotes gradient
T2931 3511-3512 . denotes .
T2932 3512-3670 sentence denotes This could reflect the binding of annexin A7 to membrane subdomains that have different lipid or lipid/protein composition as discussed by Salzer et al. [7].
T2933 3513-3517 DT denotes This
T2934 3524-3531 VB denotes reflect
T2935 3518-3523 MD denotes could
T2936 3532-3535 DT denotes the
T2937 3536-3543 NN denotes binding
T2938 3544-3546 IN denotes of
T2939 3547-3554 NN denotes annexin
T2940 3555-3557 NN denotes A7
T2941 3558-3560 IN denotes to
T2942 3561-3569 NN denotes membrane
T2943 3570-3580 NNS denotes subdomains
T2944 3581-3585 WDT denotes that
T2945 3586-3590 VBP denotes have
T2946 3591-3600 JJ denotes different
T2947 3624-3635 NN denotes composition
T2948 3601-3606 NN denotes lipid
T2949 3607-3609 CC denotes or
T2950 3610-3615 NN denotes lipid
T2951 3616-3623 NN denotes protein
T2952 3615-3616 HYPH denotes /
T2953 3636-3638 IN denotes as
T2954 3639-3648 VBN denotes discussed
T2955 3649-3651 IN denotes by
T2956 3652-3658 NNP denotes Salzer
T2957 3659-3661 FW denotes et
T2958 3662-3665 FW denotes al.
T2959 3666-3667 -LRB- denotes [
T2960 3667-3668 CD denotes 7
T2961 3668-3669 -RRB- denotes ]
T2962 3669-3670 . denotes .
T2963 3670-3748 sentence denotes Likewise, sorcin does not exhibit a homogeneous distribution in the gradient.
T2964 3671-3679 RB denotes Likewise
T2965 3697-3704 VB denotes exhibit
T2966 3679-3681 , denotes ,
T2967 3681-3687 NN denotes sorcin
T2968 3688-3692 VBZ denotes does
T2969 3693-3696 RB denotes not
T2970 3705-3706 DT denotes a
T2971 3719-3731 NN denotes distribution
T2972 3707-3718 JJ denotes homogeneous
T2973 3732-3734 IN denotes in
T2974 3735-3738 DT denotes the
T2975 3739-3747 NN denotes gradient
T2976 3747-3748 . denotes .
T2977 3748-3837 sentence denotes Moreover, it segregates into vesicles which are not associated with annexin A7 (Fig. 3).
T2978 3749-3757 RB denotes Moreover
T2979 3762-3772 VBZ denotes segregates
T2980 3757-3759 , denotes ,
T2981 3759-3761 PRP denotes it
T2982 3773-3777 IN denotes into
T2983 3778-3786 NNS denotes vesicles
T2984 3787-3792 WDT denotes which
T2985 3801-3811 VBN denotes associated
T2986 3793-3796 VBP denotes are
T2987 3797-3800 RB denotes not
T2988 3812-3816 IN denotes with
T2989 3817-3824 NN denotes annexin
T2990 3825-3827 NN denotes A7
T2991 3828-3829 -LRB- denotes (
T2992 3829-3833 NN denotes Fig.
T2993 3834-3835 CD denotes 3
T2994 3835-3836 -RRB- denotes )
T2995 3836-3837 . denotes .
T2996 3837-3930 sentence denotes We also tested platelets for annexin A7 expression and detected the 47 kDa isoform (Fig. 1).
T2997 3838-3840 PRP denotes We
T2998 3846-3852 VBD denotes tested
T2999 3841-3845 RB denotes also
T3000 3853-3862 NNS denotes platelets
T3001 3863-3866 IN denotes for
T3002 3867-3874 NN denotes annexin
T3003 3875-3877 NN denotes A7
T3004 3878-3888 NN denotes expression
T3005 3889-3892 CC denotes and
T3006 3893-3901 VBD denotes detected
T3007 3902-3905 DT denotes the
T3008 3913-3920 NN denotes isoform
T3009 3906-3908 CD denotes 47
T3010 3909-3912 NN denotes kDa
T3011 3921-3922 -LRB- denotes (
T3012 3922-3926 NN denotes Fig.
T3013 3927-3928 CD denotes 1
T3014 3928-3929 -RRB- denotes )
T3015 3929-3930 . denotes .
T9089 752-762 NN denotes Expression
T9090 763-765 IN denotes of
T9091 766-773 NN denotes annexin
T9092 774-776 NN denotes A7
T9093 777-779 IN denotes in
T9094 780-785 JJ denotes human
T9095 796-801 NNS denotes cells
T9096 786-789 JJ denotes red
T9097 790-795 NN denotes blood
T9098 802-803 -LRB- denotes (
T9099 803-804 CD denotes 1
T9100 804-805 -RRB- denotes )
T9101 806-809 CC denotes and
T9102 810-815 JJ denotes human
T9103 816-825 NNS denotes platelets
T9104 826-827 -LRB- denotes (
T9105 827-828 CD denotes 2
T9106 828-829 -RRB- denotes )
T9107 829-830 . denotes .
T9108 830-896 sentence denotes Protein homogenates were separated by SDS-PAGE (12 % acrylamide).
T9109 831-838 NN denotes Protein
T9110 839-850 NNS denotes homogenates
T9111 856-865 VBN denotes separated
T9112 851-855 VBD denotes were
T9113 866-868 IN denotes by
T9114 869-872 NN denotes SDS
T9115 873-877 NN denotes PAGE
T9116 872-873 HYPH denotes -
T9117 878-879 -LRB- denotes (
T9118 884-894 NN denotes acrylamide
T9119 879-881 CD denotes 12
T9120 882-883 NN denotes %
T9121 894-895 -RRB- denotes )
T9122 895-896 . denotes .
T9123 896-1030 sentence denotes The resulting western blot was probed with mAb 203–217, visualization was by a secondary peroxidase coupled antibody followed by ECL.
T9124 897-900 DT denotes The
T9125 919-923 NN denotes blot
T9126 901-910 VBG denotes resulting
T9127 911-918 JJ denotes western
T9128 928-934 VBN denotes probed
T9129 924-927 VBD denotes was
T9130 967-970 VBD denotes was
T9131 935-939 IN denotes with
T9132 940-943 NN denotes mAb
T9133 944-951 CD denotes 203–217
T9134 951-953 , denotes ,
T9135 953-966 NN denotes visualization
T9136 971-973 IN denotes by
T9137 974-975 DT denotes a
T9138 1005-1013 NN denotes antibody
T9139 976-985 JJ denotes secondary
T9140 986-996 NN denotes peroxidase
T9141 997-1004 VBN denotes coupled
T9142 1014-1022 VBN denotes followed
T9143 1023-1025 IN denotes by
T9144 1026-1029 NN denotes ECL
T9145 1029-1030 . denotes .
T9146 1030-1125 sentence denotes Both isoforms are detected in red blood cells, in platelets only the small isoform is present.
T9147 1031-1035 DT denotes Both
T9148 1036-1044 NNS denotes isoforms
T9149 1049-1057 VBN denotes detected
T9150 1045-1048 VBP denotes are
T9151 1114-1116 VBZ denotes is
T9152 1058-1060 IN denotes in
T9153 1061-1064 JJ denotes red
T9154 1071-1076 NNS denotes cells
T9155 1065-1070 NN denotes blood
T9156 1076-1078 , denotes ,
T9157 1078-1080 IN denotes in
T9158 1081-1090 NNS denotes platelets
T9159 1091-1095 RB denotes only
T9160 1106-1113 NN denotes isoform
T9161 1096-1099 DT denotes the
T9162 1100-1105 JJ denotes small
T9163 1117-1124 JJ denotes present
T9164 1124-1125 . denotes .
T9240 2468-2478 NN denotes Enrichment
T9241 2479-2481 IN denotes of
T9242 2482-2489 NN denotes annexin
T9243 2490-2492 NN denotes A7
T9244 2493-2496 CC denotes and
T9245 2497-2503 NN denotes sorcin
T9246 2504-2506 IN denotes in
T9247 2507-2518 NNS denotes exovesicles
T9248 2519-2526 VBN denotes derived
T9249 2527-2531 IN denotes from
T9250 2532-2535 JJ denotes red
T9251 2542-2547 NNS denotes cells
T9252 2536-2541 NN denotes blood
T9253 2547-2548 . denotes .
T9254 2548-2770 sentence denotes Vesicles from wild type (wt) and anxA7-/- mutant (ko) were generated with Ca2+/ionophore treatment, isolated by differential centrifugation and analysed by immunoblotting with mAb 203–217 and a sorcin polyclonal antibody.
T9255 2549-2557 NNS denotes Vesicles
T9256 2608-2617 VBN denotes generated
T9257 2558-2562 IN denotes from
T9258 2563-2567 JJ denotes wild
T9259 2568-2572 NN denotes type
T9260 2573-2574 -LRB- denotes (
T9261 2574-2576 NN denotes wt
T9262 2576-2577 -RRB- denotes )
T9263 2578-2581 CC denotes and
T9264 2582-2587 JJ denotes anxA7
T9265 2591-2597 NN denotes mutant
T9266 2587-2588 SYM denotes -
T9267 2588-2589 HYPH denotes /
T9268 2589-2590 SYM denotes -
T9269 2598-2599 -LRB- denotes (
T9270 2599-2601 NN denotes ko
T9271 2601-2602 -RRB- denotes )
T9272 2603-2607 VBD denotes were
T9273 2618-2622 IN denotes with
T9274 2623-2627 NN denotes Ca2+
T9275 2628-2637 NN denotes ionophore
T9276 2627-2628 HYPH denotes /
T9277 2638-2647 NN denotes treatment
T9278 2647-2649 , denotes ,
T9279 2649-2657 VBN denotes isolated
T9280 2658-2660 IN denotes by
T9281 2661-2673 JJ denotes differential
T9282 2674-2688 NN denotes centrifugation
T9283 2689-2692 CC denotes and
T9284 2693-2701 VBN denotes analysed
T9285 2702-2704 IN denotes by
T9286 2705-2719 VBG denotes immunoblotting
T9287 2720-2724 IN denotes with
T9288 2725-2728 NN denotes mAb
T9289 2729-2732 CD denotes 203
T9290 2732-2733 SYM denotes –
T9291 2733-2736 CD denotes 217
T9292 2737-2740 CC denotes and
T9293 2741-2742 DT denotes a
T9294 2761-2769 NN denotes antibody
T9295 2743-2749 NN denotes sorcin
T9296 2750-2760 JJ denotes polyclonal
T9297 2769-2770 . denotes .
T9298 2770-2839 sentence denotes In general, annexin A7 and sorcin are more abundant in nanovesicles.
T9299 2771-2773 IN denotes In
T9300 2805-2808 VBP denotes are
T9301 2774-2781 JJ denotes general
T9302 2781-2783 , denotes ,
T9303 2783-2790 NN denotes annexin
T9304 2791-2793 NN denotes A7
T9305 2794-2797 CC denotes and
T9306 2798-2804 NN denotes sorcin
T9307 2809-2813 RBR denotes more
T9308 2814-2822 JJ denotes abundant
T9309 2823-2825 IN denotes in
T9310 2826-2838 NNS denotes nanovesicles
T9311 2838-2839 . denotes .
T9312 2839-2915 sentence denotes The 51 kDa isoform of annexin A7 (arrows) is only observed in nanovesicles.
T9313 2840-2843 DT denotes The
T9314 2851-2858 NN denotes isoform
T9315 2844-2846 CD denotes 51
T9316 2847-2850 NN denotes kDa
T9317 2890-2898 VBN denotes observed
T9318 2859-2861 IN denotes of
T9319 2862-2869 NN denotes annexin
T9320 2870-2872 NN denotes A7
T9321 2873-2874 -LRB- denotes (
T9322 2874-2880 NNS denotes arrows
T9323 2880-2881 -RRB- denotes )
T9324 2882-2884 VBZ denotes is
T9325 2885-2889 RB denotes only
T9326 2899-2901 IN denotes in
T9327 2902-2914 NNS denotes nanovesicles
T9328 2914-2915 . denotes .
T9329 2915-3014 sentence denotes Samples of both vesicles types were normalized according to their acetylcholine esterase activity.
T9330 2916-2923 NNS denotes Samples
T9331 2952-2962 VBN denotes normalized
T9332 2924-2926 IN denotes of
T9333 2927-2931 DT denotes both
T9334 2941-2946 NNS denotes types
T9335 2932-2940 NNS denotes vesicles
T9336 2947-2951 VBD denotes were
T9337 2963-2972 VBG denotes according
T9338 2973-2975 IN denotes to
T9339 2976-2981 PRP$ denotes their
T9340 3005-3013 NN denotes activity
T9341 2982-2995 NN denotes acetylcholine
T9342 2996-3004 NN denotes esterase
T9343 3013-3014 . denotes .
T9344 3014-3093 sentence denotes Human red blood cells (co) were used for control and normalized independently.
T9345 3015-3020 JJ denotes Human
T9346 3031-3036 NNS denotes cells
T9347 3021-3024 JJ denotes red
T9348 3025-3030 NN denotes blood
T9349 3047-3051 VBN denotes used
T9350 3037-3038 -LRB- denotes (
T9351 3038-3040 NN denotes co
T9352 3040-3041 -RRB- denotes )
T9353 3042-3046 VBD denotes were
T9354 3052-3055 IN denotes for
T9355 3056-3063 NN denotes control
T9356 3064-3067 CC denotes and
T9357 3068-3078 VBD denotes normalized
T9358 3079-3092 RB denotes independently
T9359 3092-3093 . denotes .
T9397 3941-3952 NN denotes Association
T9398 3953-3955 IN denotes of
T9399 3956-3963 NN denotes annexin
T9400 3964-3966 NN denotes A7
T9401 3967-3970 CC denotes and
T9402 3971-3977 NN denotes sorcin
T9403 3978-3982 IN denotes with
T9404 3983-3991 JJ denotes distinct
T9405 4023-4032 NNS denotes fractions
T9406 3992-3995 JJ denotes red
T9407 4002-4006 NN denotes cell
T9408 3996-4001 NN denotes blood
T9409 4007-4013 NN denotes plasma
T9410 4014-4022 NN denotes membrane
T9411 4032-4033 . denotes .
T9412 4033-4118 sentence denotes Lysed human red blood cells were added to a self forming iodixanol density gradient.
T9413 4034-4039 VBN denotes Lysed
T9414 4056-4061 NNS denotes cells
T9415 4040-4045 JJ denotes human
T9416 4046-4049 JJ denotes red
T9417 4050-4055 NN denotes blood
T9418 4067-4072 VBN denotes added
T9419 4062-4066 VBD denotes were
T9420 4073-4075 IN denotes to
T9421 4076-4077 DT denotes a
T9422 4109-4117 NN denotes gradient
T9423 4078-4082 NN denotes self
T9424 4083-4090 VBG denotes forming
T9425 4091-4100 NN denotes iodixanol
T9426 4101-4108 NN denotes density
T9427 4117-4118 . denotes .
T9428 4118-4200 sentence denotes The density of the gradient increases from left (1.06 g/ml) to right (1.20 g/ml).
T9429 4119-4122 DT denotes The
T9430 4123-4130 NN denotes density
T9431 4147-4156 VBZ denotes increases
T9432 4131-4133 IN denotes of
T9433 4134-4137 DT denotes the
T9434 4138-4146 NN denotes gradient
T9435 4157-4161 IN denotes from
T9436 4162-4166 JJ denotes left
T9437 4167-4168 -LRB- denotes (
T9438 4173-4174 NNS denotes g
T9439 4168-4172 CD denotes 1.06
T9440 4174-4175 SYM denotes /
T9441 4175-4177 NN denotes ml
T9442 4177-4178 -RRB- denotes )
T9443 4179-4181 IN denotes to
T9444 4182-4187 JJ denotes right
T9445 4188-4189 -LRB- denotes (
T9446 4194-4195 NNS denotes g
T9447 4189-4193 CD denotes 1.20
T9448 4195-4196 SYM denotes /
T9449 4196-4198 NN denotes ml
T9450 4198-4199 -RRB- denotes )
T9451 4199-4200 . denotes .
T9452 4200-4296 sentence denotes Fractions were analysed by immunoblotting using mAb 203–217 and the sorcin polyclonal antibody.
T9453 4201-4210 NNS denotes Fractions
T9454 4216-4224 VBN denotes analysed
T9455 4211-4215 VBD denotes were
T9456 4225-4227 IN denotes by
T9457 4228-4242 NN denotes immunoblotting
T9458 4243-4248 VBG denotes using
T9459 4249-4252 NN denotes mAb
T9460 4253-4256 CD denotes 203
T9461 4256-4257 SYM denotes –
T9462 4257-4260 CD denotes 217
T9463 4261-4264 CC denotes and
T9464 4265-4268 DT denotes the
T9465 4287-4295 NN denotes antibody
T9466 4269-4275 NN denotes sorcin
T9467 4276-4286 JJ denotes polyclonal
T9468 4295-4296 . denotes .
T2865 3123-3130 NN denotes annexin
T2866 3131-3133 NN denotes A7
T2867 3134-3136 IN denotes in
T2868 3137-3140 JJ denotes red
T2869 3147-3152 NNS denotes cells
T2870 3141-3146 NN denotes blood
T2871 3153-3155 PRP denotes we
T2872 3161-3165 NN denotes self
T2873 3166-3173 VBG denotes forming
T2874 3192-3201 NNS denotes gradients
T2554 551-558 NN denotes isoform
T2555 544-546 CD denotes 47
T2556 547-550 NN denotes kDa
T2557 563-571 VBN denotes detected
T2558 559-562 VBD denotes was
T2559 572-574 IN denotes in
T2560 575-581 NN denotes silver
T2561 582-589 VBN denotes stained
T2562 590-594 NNS denotes gels
T2563 595-598 CC denotes and
T2564 599-602 PRP$ denotes its
T2565 603-611 NN denotes identity
T2566 628-637 VBN denotes confirmed
T2567 612-616 IN denotes with
T2568 617-624 NN denotes annexin
T2569 625-627 NN denotes A7
T2570 638-640 IN denotes by
T2571 641-648 NN denotes peptide
T2572 654-668 NN denotes fingerprinting
T2573 649-653 NN denotes mass
T2574 669-670 -LRB- denotes (
T2575 679-684 VBN denotes shown
T2576 670-674 NNS denotes data
T2577 675-678 RB denotes not
T2578 684-685 -RRB- denotes )
T2579 685-686 . denotes .
T2580 686-741 sentence denotes The 51 kDa isoform was only detected in western blots.
T2581 687-690 DT denotes The
T2582 698-705 NN denotes isoform
T2583 691-693 CD denotes 51
T2584 694-697 NN denotes kDa
T2585 715-723 VBN denotes detected
T2586 706-709 VBD denotes was
T2587 710-714 RB denotes only
T2588 724-726 IN denotes in
T2589 727-734 JJ denotes western
T2590 735-740 NNS denotes blots
T2591 740-741 . denotes .
T2592 741-742 sentence denotes
T2593 1126-1283 sentence denotes Its presence in vesicles led to the suggestion, that, along with raft domains, annexin A7 plays a role in membrane organization and the vesiculation process.
T2594 1126-1129 PRP$ denotes Its
T2595 1130-1138 NN denotes presence
T2596 1151-1154 VBD denotes led
T2597 1139-1141 IN denotes in
T2598 1142-1150 NNS denotes vesicles
T2599 1155-1157 IN denotes to
T2600 1158-1161 DT denotes the
T2601 1162-1172 NN denotes suggestion
T2602 1172-1174 , denotes ,
T2603 1174-1178 IN denotes that
T2604 1216-1221 VBZ denotes plays
T2605 1178-1180 , denotes ,
T2875 3174-3183 NN denotes iodixanol
T2792 2135-2136 . denotes .
T2793 2136-2300 sentence denotes It appears that annexin A7, although it has been described to fuse membranes, is not a key component in the process of the formation of red blood cell exovesicles.
T2794 2137-2139 PRP denotes It
T2795 2140-2147 VBZ denotes appears
T2796 2148-2152 IN denotes that
T2797 2215-2217 VBZ denotes is
T2798 2153-2160 NN denotes annexin
T2799 2161-2163 NN denotes A7
T2800 2163-2165 , denotes ,
T2801 2165-2173 IN denotes although
T2802 2186-2195 VBN denotes described
T2803 2174-2176 PRP denotes it
T2804 2177-2180 VBZ denotes has
T2805 2181-2185 VBN denotes been
T2806 2196-2198 TO denotes to
T2807 2199-2203 VB denotes fuse
T2808 2204-2213 NNS denotes membranes
T2809 2213-2215 , denotes ,
T2810 2218-2221 RB denotes not
T2811 2222-2223 DT denotes a
T2812 2228-2237 NN denotes component
T2813 2224-2227 JJ denotes key
T2814 2238-2240 IN denotes in
T2815 2241-2244 DT denotes the
T2816 2245-2252 NN denotes process
T2817 2253-2255 IN denotes of
T2818 2256-2259 DT denotes the
T2819 2260-2269 NN denotes formation
T2820 2270-2272 IN denotes of
T2821 2273-2276 JJ denotes red
T2822 2283-2287 NN denotes cell
T2823 2277-2282 NN denotes blood
T2824 2288-2299 NNS denotes exovesicles
T2825 2299-2300 . denotes .
T2826 2300-2457 sentence denotes When we probed for the presence of sorcin in wild type and mutant vesicles we found that the sorcin levels were reduced in the mutant nanovesicles (Fig. 2).
T2827 2301-2305 WRB denotes When
T2828 2309-2315 VBD denotes probed
T2829 2306-2308 PRP denotes we
T2830 2379-2384 VBD denotes found
T2831 2316-2319 IN denotes for
T2858 2457-3202 sentence denotes Figure 2 Enrichment of annexin A7 and sorcin in exovesicles derived from red blood cells. Vesicles from wild type (wt) and anxA7-/- mutant (ko) were generated with Ca2+/ionophore treatment, isolated by differential centrifugation and analysed by immunoblotting with mAb 203–217 and a sorcin polyclonal antibody. In general, annexin A7 and sorcin are more abundant in nanovesicles. The 51 kDa isoform of annexin A7 (arrows) is only observed in nanovesicles. Samples of both vesicles types were normalized according to their acetylcholine esterase activity. Human red blood cells (co) were used for control and normalized independently. To study the distribution of annexin A7 in red blood cells we used self forming iodixanol density gradients.
T2859 3094-3096 TO denotes To
T2860 3097-3102 VB denotes study
T2861 3156-3160 VBD denotes used
T2862 3103-3106 DT denotes the
T2863 3107-3119 NN denotes distribution
T2864 3120-3122 IN denotes of
T2876 3184-3191 NN denotes density
T2877 3201-3202 . denotes .
T2878 3202-3346 sentence denotes Both isoforms were present in the soluble fraction as well as in the gradient fractions where they are assumed to be associated with membranes.
T2879 3203-3207 DT denotes Both
R1264 T2443 T2444 compound Annexin,A7
R1265 T2444 T2445 compound A7,expression
R1266 T2446 T2445 prep in,expression
R1267 T2447 T2448 amod red,cells
R1268 T2448 T2446 pobj cells,in
R1269 T2449 T2448 compound blood,cells
R1270 T2450 T2448 punct ", ",cells
R1271 T2451 T2452 amod red,cell
R1272 T2452 T2454 npadvmod cell,derived
R1273 T2453 T2452 compound blood,cell
R1274 T2454 T2455 amod derived,exovesicles
R1275 T2455 T2448 appos exovesicles,cells
R1276 T2456 T2446 cc and,in
R1277 T2457 T2446 conj in,in
R1278 T2458 T2457 pobj platelets,in
R1279 T2460 T2461 nsubj Salzer,reported
R1280 T2462 T2463 advmod et,al.
R1281 T2463 T2460 advmod al.,Salzer
R1282 T2464 T2461 advmod recently,reported
R1283 T2465 T2466 det the,presence
R1284 T2466 T2461 dobj presence,reported
R1285 T2467 T2466 prep of,presence
R1286 T2468 T2469 det the,isoform
R1287 T2469 T2467 pobj isoform,of
R1288 T2470 T2471 nummod 47,kDa
R1289 T2471 T2469 compound kDa,isoform
R1290 T2472 T2469 prep of,isoform
R1291 T2473 T2474 compound annexin,A7
R1292 T2474 T2472 pobj A7,of
R1293 T2475 T2474 cc and,A7
R1294 T2476 T2477 poss its,partner
R1295 T2477 T2474 conj partner,A7
R1296 T2478 T2477 appos sorcin,partner
R1297 T2479 T2469 prep in,isoform
R1298 T2480 T2479 pobj micro,in
R1299 T2481 T2480 punct -,micro
R1300 T2482 T2480 cc and,micro
R1301 T2483 T2480 conj nanovesicles,micro
R1302 T2484 T2480 acl derived,micro
R1303 T2485 T2484 prep from,derived
R1304 T2486 T2487 amod red,cells
R1305 T2487 T2485 pobj cells,from
R1306 T2488 T2487 compound blood,cells
R1307 T2489 T2490 advmod where,located
R1308 T2490 T2487 relcl located,cells
R1309 T2491 T2490 nsubjpass they,located
R1310 T2492 T2490 auxpass are,located
R1311 T2493 T2490 prep in,located
R1312 T2494 T2495 det the,lumen
R1313 T2495 T2493 pobj lumen,in
R1314 T2496 T2490 cc and,located
R1315 T2497 T2498 auxpass are,enriched
R1316 T2498 T2490 conj enriched,located
R1317 T2499 T2498 advmod also,enriched
R1318 T2500 T2498 prep in,enriched
R1319 T2501 T2502 compound membrane,rafts
R1320 T2502 T2500 pobj rafts,in
R1321 T2503 T2504 punct [,7
R1322 T2504 T2461 parataxis 7,reported
R1323 T2505 T2504 punct ],7
R1324 T2506 T2461 punct .,reported
R1325 T2508 T2509 nsubj We,extended
R1326 T2510 T2509 aux have,extended
R1327 T2511 T2509 advmod now,extended
R1335 T2519 T2520 det the,isoform
R1336 T2520 T2518 nsubj isoform,is
R1337 T2521 T2522 nummod 51,kDa
R1338 T2522 T2520 compound kDa,isoform
R1339 T2523 T2518 acomp present,is
R1340 T2524 T2525 advmod as,well
R1341 T2525 T2518 advmod well,is
R1342 T2526 T2509 punct .,extended
R1343 T2528 T2529 prep In,detected
R1344 T2530 T2531 amod western,blots
R1345 T2531 T2528 pobj blots,In
R1346 T2532 T2531 prep of,blots
R1347 T2533 T2534 amod human,cells
R1348 T2534 T2532 pobj cells,of
R1349 T2535 T2534 amod red,cells
R1350 T2536 T2534 compound blood,cells
R1351 T2537 T2538 det the,isoforms
R1352 T2538 T2529 nsubjpass isoforms,detected
R1353 T2539 T2540 nummod 47,kDa
R1354 T2540 T2538 compound kDa,isoforms
R1355 T2541 T2539 cc and,47
R1356 T2542 T2539 conj 51,47
R1357 T2543 T2529 auxpass were,detected
R1358 T2544 T2529 advcl using,detected
R1359 T2545 T2544 dobj mAb,using
R1360 T2546 T2545 nummod 203–217,mAb
R1361 T2547 T2548 punct (,Fig.
R1362 T2548 T2529 parataxis Fig.,detected
R1363 T2549 T2548 nummod 1,Fig.
R1364 T2550 T2548 punct ),Fig.
R1365 T2551 T2529 punct .,detected
R1366 T2553 T2554 det The,isoform
R1367 T2554 T2557 nsubjpass isoform,detected
R1368 T2555 T2556 nummod 47,kDa
R1369 T2556 T2554 compound kDa,isoform
R1370 T2558 T2557 auxpass was,detected
R1371 T2559 T2557 prep in,detected
R1372 T2560 T2561 npadvmod silver,stained
R1373 T2561 T2562 amod stained,gels
R1374 T2562 T2559 pobj gels,in
R1375 T2563 T2557 cc and,detected
R1376 T2564 T2565 poss its,identity
R1377 T2565 T2566 nsubj identity,confirmed
R1378 T2566 T2557 conj confirmed,detected
R1379 T2567 T2565 prep with,identity
R1380 T2568 T2569 compound annexin,A7
R1381 T2569 T2567 pobj A7,with
R1382 T2570 T2566 prep by,confirmed
R1383 T2571 T2572 compound peptide,fingerprinting
R1384 T2572 T2570 pobj fingerprinting,by
R1385 T2573 T2572 compound mass,fingerprinting
R1386 T2574 T2575 punct (,shown
R1387 T2575 T2566 parataxis shown,confirmed
R1388 T2576 T2575 nsubj data,shown
R1389 T2577 T2575 neg not,shown
R1390 T2578 T2575 punct ),shown
R1391 T2579 T2566 punct .,confirmed
R1392 T2581 T2582 det The,isoform
R1393 T2582 T2585 nsubjpass isoform,detected
R1394 T2583 T2584 nummod 51,kDa
R1395 T2584 T2582 compound kDa,isoform
R1396 T2586 T2585 auxpass was,detected
R1397 T2587 T2585 advmod only,detected
R1398 T2588 T2585 prep in,detected
R1399 T2589 T2590 amod western,blots
R1400 T2590 T2588 pobj blots,in
R1401 T2591 T2585 punct .,detected
R1420 T2613 T2614 det a,role
R1421 T2614 T2604 dobj role,plays
R1422 T2615 T2604 prep in,plays
R1423 T2616 T2617 compound membrane,organization
R1424 T2617 T2615 pobj organization,in
R1425 T2618 T2617 cc and,organization
R1426 T2619 T2620 det the,process
R1427 T2620 T2617 conj process,organization
R1428 T2621 T2620 compound vesiculation,process
R1429 T2622 T2596 punct .,led
R1430 T2624 T2625 aux To,examine
R1431 T2625 T2626 advcl examine,analysed
R1432 T2627 T2625 dobj this,examine
R1433 T2628 T2626 punct ", ",analysed
R1434 T2629 T2626 nsubj we,analysed
R1435 T2630 T2631 det the,ability
R1436 T2631 T2626 dobj ability,analysed
R1437 T2632 T2631 prep of,ability
R1438 T2633 T2634 amod red,cells
R1439 T2634 T2632 pobj cells,of
R1440 T2635 T2634 compound blood,cells
R1441 T2636 T2634 acl derived,cells
R1442 T2637 T2636 prep from,derived
R1443 T2638 T2639 det the,mice
R1444 T2639 T2637 pobj mice,from
R1445 T2640 T2641 compound annexin,A7
R1446 T2641 T2639 compound A7,mice
R1447 T2642 T2643 compound knock,out
R1448 T2643 T2639 compound out,mice
R1449 T2644 T2645 punct (,anxA7
R1450 T2645 T2639 parataxis anxA7,mice
R1451 T2646 T2645 punct -,anxA7
R1452 T2647 T2645 punct /,anxA7
R1453 T2648 T2645 punct -,anxA7
R1454 T2649 T2645 punct ),anxA7
R1455 T2650 T2651 aux to,form
R1456 T2651 T2631 acl form,ability
R1457 T2652 T2651 dobj exovesicles,form
R1458 T2653 T2626 punct .,analysed
R1459 T2655 T2656 prep Because,included
R1460 T2657 T2655 pcomp of,Because
R1461 T2658 T2659 amod better,accessibility
R1462 T2659 T2655 pobj accessibility,Because
R1463 T2660 T2659 cc and,accessibility
R1464 T2661 T2662 amod larger,amounts
R1465 T2662 T2659 conj amounts,accessibility
R1466 T2663 T2662 amod available,amounts
R1467 T2664 T2665 dep that,led
R1468 T2665 T2659 relcl led,accessibility
R1469 T2666 T2665 prep to,led
R1470 T2667 T2668 amod clear,results
R1471 T2668 T2666 pobj results,to
R1472 T2669 T2656 punct ", ",included
R1473 T2670 T2656 nsubj we,included
R1474 T2671 T2672 poss our,data
R1475 T2672 T2656 dobj data,included
R1476 T2673 T2672 acl obtained,data
R1477 T2674 T2673 prep with,obtained
R1478 T2675 T2676 amod human,blood
R1479 T2676 T2674 pobj blood,with
R1480 T2677 T2656 punct .,included
R1481 T2679 T2680 advcl Independent,released
R1482 T2681 T2679 prep of,Independent
R1483 T2682 T2683 det the,presence
R1484 T2683 T2681 pobj presence,of
R1485 T2684 T2683 prep of,presence
R1486 T2685 T2686 compound annexin,A7
R1487 T2686 T2684 pobj A7,of
R1488 T2687 T2688 det both,types
R1489 T2688 T2680 nsubjpass types,released
R1490 T2689 T2688 prep of,types
R1491 T2690 T2689 pobj exovesicles,of
R1492 T2691 T2688 punct ", ",types
R1493 T2692 T2693 nmod micro,nanovesicles
R1494 T2693 T2688 appos nanovesicles,types
R1495 T2694 T2693 punct -,nanovesicles
R1496 T2695 T2693 cc and,nanovesicles
R1497 T2696 T2680 punct ", ",released
R1498 T2697 T2680 auxpass were,released
R1499 T2698 T2680 prep after,released
R1500 T2699 T2700 compound Ca2+,ionophore
R1501 T2700 T2702 compound ionophore,treatment
R1502 T2701 T2700 punct /,ionophore
R1503 T2702 T2698 pobj treatment,after
R1504 T2703 T2704 mark as,determined
R1505 T2704 T2680 advcl determined,released
R1506 T2705 T2704 agent by,determined
R1507 T2706 T2707 compound acetylcholine,esterase
R1508 T2707 T2708 compound esterase,activity
R1509 T2708 T2705 pobj activity,by
R1510 T2709 T2708 cc and,activity
R1511 T2710 T2711 amod microscopic,examination
R1512 T2711 T2708 conj examination,activity
R1513 T2712 T2680 punct .,released
R1514 T2714 T2715 compound Annexin,A7
R1515 T2715 T2716 nsubj A7,is
R1516 T2717 T2716 acomp present,is
R1517 T2718 T2716 prep in,is
R1518 T2719 T2720 preconj both,types
R1519 T2720 T2718 pobj types,in
R1520 T2721 T2720 compound vesicle,types
R1521 T2722 T2723 advmod where,is
R1522 T2723 T2720 advcl is,types
R1523 T2724 T2723 nsubj it,is
R1524 T2725 T2726 advmod more,enriched
R1525 T2726 T2723 acomp enriched,is
R1526 T2727 T2723 prep in,is
R1527 T2728 T2727 pobj nanovesicles,in
R1528 T2729 T2730 punct (,Fig.
R1529 T2730 T2716 parataxis Fig.,is
R1530 T2731 T2730 nummod 2,Fig.
R1531 T2732 T2730 punct ),Fig.
R1532 T2733 T2716 punct .,is
R1533 T2735 T2736 det The,isoform
R1534 T2736 T2739 nsubj isoform,is
R1535 T2737 T2738 nummod 51,kDa
R1536 T2738 T2736 compound kDa,isoform
R1537 T2740 T2739 advmod only,is
R1538 T2741 T2739 acomp detectable,is
R1539 T2742 T2739 prep in,is
R1540 T2743 T2742 pobj nanovesicles,in
R1541 T2744 T2739 punct .,is
R1542 T2746 T2747 det The,quantity
R1543 T2747 T2748 nsubj quantity,differ
R1544 T2749 T2747 prep of,quantity
R1545 T2750 T2751 det both,types
R1546 T2751 T2749 pobj types,of
R1547 T2752 T2751 compound vesicle,types
R1548 T2753 T2748 aux did,differ
R1549 T2754 T2748 neg not,differ
R1550 T2755 T2748 prep between,differ
R1551 T2756 T2757 amod wild,type
R1552 T2757 T2758 nmod type,vesicles
R1553 T2758 T2755 pobj vesicles,between
R1554 T2759 T2757 cc and,type
R1555 T2760 T2761 compound knock,out
R1556 T2761 T2757 conj out,type
R1557 T2762 T2763 amod red,cell
R1558 T2763 T2758 compound cell,vesicles
R1559 T2764 T2763 compound blood,cell
R1560 T2765 T2766 mark as,determined
R1561 T2766 T2748 advcl determined,differ
R1562 T2767 T2766 agent by,determined
R1563 T2768 T2769 det the,values
R1564 T2769 T2767 pobj values,by
R1565 T2770 T2769 compound AChE,values
R1566 T2771 T2769 punct -,values
R1567 T2772 T2773 punct (,both
R1568 T2773 T2769 parataxis both,values
R1569 T2774 T2775 compound A405,nanovesicles
R1570 T2775 T2776 dep nanovesicles,0.18
R1571 T2776 T2773 dep 0.18,both
R1572 T2777 T2776 punct : ,0.18
R1573 T2778 T2776 punct ~,0.18
R1574 T2779 T2773 punct ;,both
R1575 T2780 T2781 compound A405,microvesicles
R1576 T2781 T2782 dep microvesicles,0.9
R1577 T2782 T2773 dep 0.9,both
R1578 T2783 T2782 punct : ,0.9
R1579 T2784 T2782 punct ~,0.9
R1580 T2785 T2773 punct ;,both
R1581 T2786 T2773 punct ", ",both
R1596 T2803 T2802 nsubjpass it,described
R1597 T2804 T2802 aux has,described
R1598 T2805 T2802 auxpass been,described
R1599 T2806 T2807 aux to,fuse
R1600 T2807 T2802 xcomp fuse,described
R1601 T2808 T2807 dobj membranes,fuse
R1602 T2809 T2797 punct ", ",is
R1603 T2810 T2797 neg not,is
R1604 T2811 T2812 det a,component
R1605 T2812 T2797 attr component,is
R1606 T2813 T2812 amod key,component
R1607 T2814 T2812 prep in,component
R1608 T2815 T2816 det the,process
R1609 T2816 T2814 pobj process,in
R1610 T2817 T2816 prep of,process
R1611 T2818 T2819 det the,formation
R1612 T2819 T2817 pobj formation,of
R1613 T2820 T2819 prep of,formation
R1614 T2821 T2822 amod red,cell
R1615 T2822 T2824 compound cell,exovesicles
R1616 T2823 T2822 compound blood,cell
R1617 T2824 T2820 pobj exovesicles,of
R1618 T2825 T2795 punct .,appears
R1619 T2827 T2828 advmod When,probed
R1620 T2828 T2830 advcl probed,found
R1621 T2829 T2828 nsubj we,probed
R1622 T2831 T2828 prep for,probed
R1623 T2832 T2833 det the,presence
R1624 T2833 T2831 pobj presence,for
R1625 T2834 T2833 prep of,presence
R1626 T2835 T2834 pobj sorcin,of
R1627 T2836 T2828 prep in,probed
R1628 T2837 T2838 amod wild,type
R1629 T2838 T2839 nmod type,vesicles
R1630 T2839 T2836 pobj vesicles,in
R1631 T2840 T2838 cc and,type
R1632 T2841 T2838 conj mutant,type
R1633 T2842 T2830 nsubj we,found
R1634 T2843 T2844 mark that,reduced
R1635 T2844 T2830 ccomp reduced,found
R1636 T2845 T2846 det the,levels
R1637 T2846 T2844 nsubjpass levels,reduced
R1638 T2847 T2846 compound sorcin,levels
R1639 T2848 T2844 auxpass were,reduced
R1640 T2849 T2844 prep in,reduced
R1641 T2850 T2851 det the,nanovesicles
R1642 T2851 T2849 pobj nanovesicles,in
R1643 T2852 T2851 amod mutant,nanovesicles
R1644 T2853 T2854 punct (,Fig.
R1645 T2854 T2830 parataxis Fig.,found
R1646 T2855 T2854 nummod 2,Fig.
R1647 T2856 T2854 punct ),Fig.
R1648 T2857 T2830 punct .,found
R1664 T2875 T2874 compound iodixanol,gradients
R1665 T2876 T2874 compound density,gradients
R1666 T2877 T2861 punct .,used
R1667 T2879 T2880 det Both,isoforms
R1668 T2880 T2881 nsubj isoforms,were
R1669 T2882 T2881 acomp present,were
R1670 T2883 T2881 prep in,were
R1671 T2884 T2885 det the,fraction
R1672 T2885 T2883 pobj fraction,in
R1673 T2886 T2885 amod soluble,fraction
R1674 T2887 T2888 advmod as,as
R1675 T2888 T2883 cc as,in
R1676 T2889 T2888 advmod well,as
R1677 T2890 T2883 conj in,in
R1678 T2891 T2892 det the,fractions
R1679 T2892 T2890 pobj fractions,in
R1680 T2893 T2892 compound gradient,fractions
R1681 T2894 T2895 advmod where,assumed
R1682 T2895 T2892 relcl assumed,fractions
R1683 T2896 T2895 nsubjpass they,assumed
R1684 T2897 T2895 auxpass are,assumed
R1685 T2898 T2899 aux to,associated
R1686 T2899 T2895 xcomp associated,assumed
R1687 T2900 T2899 auxpass be,associated
R1688 T2901 T2899 prep with,associated
R1689 T2902 T2901 pobj membranes,with
R1690 T2903 T2881 punct .,were
R1691 T2905 T2906 det These,membranes
R1692 T2906 T2907 nsubj membranes,are
R1693 T2908 T2907 advmod exclusively,are
R1694 T2909 T2910 compound plasma,membranes
R1695 T2910 T2907 attr membranes,are
R1696 T2911 T2912 mark as,are
R1697 T2912 T2907 advcl are,are
R1698 T2913 T2914 amod red,cells
R1699 T2914 T2912 nsubj cells,are
R1700 T2915 T2914 compound blood,cells
R1701 T2916 T2912 acomp free,are
R1702 T2917 T2916 prep of,free
R1703 T2918 T2919 det any,organelles
R1704 T2919 T2917 pobj organelles,of
R1705 T2920 T2907 punct .,are
R1706 T2922 T2923 det The,distribution
R1707 T2923 T2924 nsubj distribution,was
R1708 T2925 T2924 advmod however,was
R1709 T2926 T2924 neg not,was
R1710 T2927 T2924 acomp homogeneous,was
R1711 T2928 T2924 prep throughout,was
R1712 T2929 T2930 det the,gradient
R1713 T2930 T2928 pobj gradient,throughout
R1714 T2931 T2924 punct .,was
R1715 T2933 T2934 nsubj This,reflect
R1716 T2935 T2934 aux could,reflect
R1717 T2936 T2937 det the,binding
R1718 T2937 T2934 dobj binding,reflect
R1719 T2938 T2937 prep of,binding
R1720 T2939 T2940 compound annexin,A7
R1721 T2940 T2938 pobj A7,of
R1722 T2941 T2937 prep to,binding
R1723 T2942 T2943 compound membrane,subdomains
R1724 T2943 T2941 pobj subdomains,to
R1725 T2944 T2945 dep that,have
R1726 T2945 T2943 relcl have,subdomains
R1727 T2946 T2947 amod different,composition
R1728 T2947 T2945 dobj composition,have
R1729 T2948 T2947 nmod lipid,composition
R1730 T2949 T2948 cc or,lipid
R1731 T2950 T2951 compound lipid,protein
R1732 T2951 T2948 conj protein,lipid
R1733 T2952 T2951 punct /,protein
R1734 T2953 T2954 mark as,discussed
R1735 T2954 T2934 advcl discussed,reflect
R1736 T2955 T2954 agent by,discussed
R1737 T2956 T2955 pobj Salzer,by
R1738 T2957 T2958 advmod et,al.
R1739 T2958 T2956 advmod al.,Salzer
R1740 T2959 T2960 punct [,7
R1741 T2960 T2934 parataxis 7,reflect
R1742 T2961 T2960 punct ],7
R1743 T2962 T2934 punct .,reflect
R1744 T2964 T2965 advmod Likewise,exhibit
R1745 T2966 T2965 punct ", ",exhibit
R1746 T2967 T2965 nsubj sorcin,exhibit
R1747 T2968 T2965 aux does,exhibit
R1748 T2969 T2965 neg not,exhibit
R1749 T2970 T2971 det a,distribution
R1750 T2971 T2965 dobj distribution,exhibit
R1751 T2972 T2971 amod homogeneous,distribution
R1752 T2973 T2965 prep in,exhibit
R1753 T2974 T2975 det the,gradient
R1754 T2975 T2973 pobj gradient,in
R1755 T2976 T2965 punct .,exhibit
R1756 T2978 T2979 advmod Moreover,segregates
R1757 T2980 T2979 punct ", ",segregates
R1758 T2981 T2979 nsubj it,segregates
R1759 T2982 T2979 prep into,segregates
R1760 T2983 T2982 pobj vesicles,into
R1761 T2984 T2985 dep which,associated
R1762 T2985 T2983 relcl associated,vesicles
R1763 T2986 T2985 auxpass are,associated
R1764 T2987 T2985 neg not,associated
R1765 T2988 T2985 prep with,associated
R1766 T2989 T2990 compound annexin,A7
R1767 T2990 T2988 pobj A7,with
R1768 T2991 T2992 punct (,Fig.
R1769 T2992 T2979 parataxis Fig.,segregates
R1770 T2993 T2992 nummod 3,Fig.
R1771 T2994 T2992 punct ),Fig.
R1772 T2995 T2979 punct .,segregates
R1773 T2997 T2998 nsubj We,tested
R1774 T2999 T2998 advmod also,tested
R1775 T3000 T2998 dobj platelets,tested
R1776 T3001 T2998 prep for,tested
R1777 T3002 T3003 compound annexin,A7
R1778 T3003 T3004 compound A7,expression
R1779 T3004 T3001 pobj expression,for
R1780 T3005 T2998 cc and,tested
R1781 T3006 T2998 conj detected,tested
R1782 T3007 T3008 det the,isoform
R1783 T3008 T3006 dobj isoform,detected
R1784 T3009 T3010 nummod 47,kDa
R1785 T3010 T3008 compound kDa,isoform
R1786 T3011 T3012 punct (,Fig.
R1787 T3012 T3006 parataxis Fig.,detected
R1788 T3013 T3012 nummod 1,Fig.
R1789 T3014 T3012 punct ),Fig.
R1790 T3015 T2998 punct .,tested
R5713 T9090 T9089 prep of,Expression
R5714 T9091 T9092 compound annexin,A7
R5715 T9092 T9090 pobj A7,of
R5716 T9093 T9089 prep in,Expression
R5717 T9094 T9095 amod human,cells
R5718 T9095 T9093 pobj cells,in
R5719 T9096 T9095 amod red,cells
R5720 T9097 T9095 compound blood,cells
R5721 T9098 T9099 punct (,1
R5722 T9099 T9095 parataxis 1,cells
R5723 T9100 T9099 punct ),1
R5724 T9101 T9095 cc and,cells
R5725 T9102 T9103 amod human,platelets
R5726 T9103 T9095 conj platelets,cells
R5727 T9104 T9105 punct (,2
R5728 T9105 T9103 parataxis 2,platelets
R5729 T9106 T9105 punct ),2
R5730 T9107 T9089 punct .,Expression
R5731 T9109 T9110 compound Protein,homogenates
R5732 T9110 T9111 nsubjpass homogenates,separated
R5733 T9112 T9111 auxpass were,separated
R5734 T9113 T9111 prep by,separated
R5735 T9114 T9115 compound SDS,PAGE
R5736 T9115 T9113 pobj PAGE,by
R5737 T9116 T9115 punct -,PAGE
R5738 T9117 T9118 punct (,acrylamide
R5739 T9118 T9111 parataxis acrylamide,separated
R5740 T9119 T9120 nummod 12,%
R5741 T9120 T9118 compound %,acrylamide
R5742 T9121 T9118 punct ),acrylamide
R5743 T9122 T9111 punct .,separated
R5744 T9124 T9125 det The,blot
R5745 T9125 T9128 nsubjpass blot,probed
R5746 T9126 T9125 amod resulting,blot
R5747 T9127 T9125 amod western,blot
R5748 T9128 T9130 ccomp probed,was
R5749 T9129 T9128 auxpass was,probed
R5750 T9131 T9128 prep with,probed
R5751 T9132 T9131 pobj mAb,with
R5752 T9133 T9132 nummod 203–217,mAb
R5753 T9134 T9130 punct ", ",was
R5754 T9135 T9130 nsubj visualization,was
R5755 T9136 T9130 prep by,was
R5756 T9137 T9138 det a,antibody
R5757 T9138 T9136 pobj antibody,by
R5758 T9139 T9138 amod secondary,antibody
R5759 T9140 T9141 npadvmod peroxidase,coupled
R5760 T9141 T9138 amod coupled,antibody
R5761 T9142 T9138 acl followed,antibody
R5762 T9143 T9142 agent by,followed
R5763 T9144 T9143 pobj ECL,by
R5764 T9145 T9130 punct .,was
R5765 T9147 T9148 det Both,isoforms
R5766 T9148 T9149 nsubjpass isoforms,detected
R5767 T9149 T9151 ccomp detected,is
R5768 T9150 T9149 auxpass are,detected
R5769 T9152 T9149 prep in,detected
R5770 T9153 T9154 amod red,cells
R5771 T9154 T9152 pobj cells,in
R5772 T9155 T9154 compound blood,cells
R5773 T9156 T9151 punct ", ",is
R5774 T9157 T9151 prep in,is
R5775 T9158 T9157 pobj platelets,in
R5776 T9159 T9160 advmod only,isoform
R5777 T9160 T9151 nsubj isoform,is
R5778 T9161 T9160 det the,isoform
R5779 T9162 T9160 amod small,isoform
R5780 T9163 T9151 acomp present,is
R5781 T9164 T9151 punct .,is
R5782 T9241 T9240 prep of,Enrichment
R5783 T9242 T9243 compound annexin,A7
R5784 T9243 T9241 pobj A7,of
R5785 T9244 T9243 cc and,A7
R5786 T9245 T9243 conj sorcin,A7
R5787 T9246 T9240 prep in,Enrichment
R5788 T9247 T9246 pobj exovesicles,in
R5789 T9248 T9247 acl derived,exovesicles
R5790 T9249 T9248 prep from,derived
R5791 T9250 T9251 amod red,cells
R5792 T9251 T9249 pobj cells,from
R5793 T9252 T9251 compound blood,cells
R5794 T9253 T9240 punct .,Enrichment
R5795 T9255 T9256 nsubjpass Vesicles,generated
R5796 T9257 T9255 prep from,Vesicles
R5797 T9258 T9259 amod wild,type
R5798 T9259 T9257 pobj type,from
R5799 T9260 T9259 punct (,type
R5800 T9261 T9259 appos wt,type
R5801 T9262 T9259 punct ),type
R5802 T9263 T9259 cc and,type
R5803 T9264 T9265 nmod anxA7,mutant
R5804 T9265 T9259 conj mutant,type
R5805 T9266 T9264 punct -,anxA7
R5806 T9267 T9264 punct /,anxA7
R5807 T9268 T9264 punct -,anxA7
R5808 T9269 T9265 punct (,mutant
R5809 T9270 T9265 appos ko,mutant
R5810 T9271 T9256 punct ),generated
R5811 T9272 T9256 auxpass were,generated
R5812 T9273 T9256 prep with,generated
R5813 T9274 T9275 compound Ca2+,ionophore
R5814 T9275 T9277 compound ionophore,treatment
R5815 T9276 T9275 punct /,ionophore
R5816 T9277 T9273 pobj treatment,with
R5817 T9278 T9256 punct ", ",generated
R5818 T9279 T9256 conj isolated,generated
R5819 T9280 T9279 prep by,isolated
R5820 T9281 T9282 amod differential,centrifugation
R5821 T9282 T9280 pobj centrifugation,by
R5822 T9283 T9279 cc and,isolated
R5823 T9284 T9279 conj analysed,isolated
R5824 T9285 T9284 prep by,analysed
R5825 T9286 T9285 pobj immunoblotting,by
R5826 T9287 T9284 prep with,analysed
R5827 T9288 T9289 nmod mAb,203
R5828 T9289 T9287 pobj 203,with
R5829 T9290 T9291 punct –,217
R5830 T9291 T9289 prep 217,203
R5831 T9292 T9289 cc and,203
R5832 T9293 T9294 det a,antibody
R5833 T9294 T9289 conj antibody,203
R5834 T9295 T9294 nmod sorcin,antibody
R5835 T9296 T9294 amod polyclonal,antibody
R5836 T9297 T9256 punct .,generated
R5837 T9299 T9300 prep In,are
R5838 T9301 T9299 amod general,In
R5839 T9302 T9300 punct ", ",are
R5840 T9303 T9304 compound annexin,A7
R5841 T9304 T9300 nsubj A7,are
R5842 T9305 T9304 cc and,A7
R5843 T9306 T9304 conj sorcin,A7
R5844 T9307 T9308 advmod more,abundant
R5845 T9308 T9300 acomp abundant,are
R5846 T9309 T9300 prep in,are
R5847 T9310 T9309 pobj nanovesicles,in
R5848 T9311 T9300 punct .,are
R5849 T9313 T9314 det The,isoform
R5850 T9314 T9317 nsubjpass isoform,observed
R5851 T9315 T9316 nummod 51,kDa
R5852 T9316 T9314 compound kDa,isoform
R5853 T9318 T9314 prep of,isoform
R5854 T9319 T9320 compound annexin,A7
R5855 T9320 T9318 pobj A7,of
R5856 T9321 T9322 punct (,arrows
R5857 T9322 T9314 parataxis arrows,isoform
R5858 T9323 T9322 punct ),arrows
R5859 T9324 T9317 auxpass is,observed
R5860 T9325 T9317 advmod only,observed
R5861 T9326 T9317 prep in,observed
R5862 T9327 T9326 pobj nanovesicles,in
R5863 T9328 T9317 punct .,observed
R5864 T9330 T9331 nsubjpass Samples,normalized
R5865 T9332 T9330 prep of,Samples
R5866 T9333 T9334 det both,types
R5867 T9334 T9332 pobj types,of
R5868 T9335 T9334 compound vesicles,types
R5869 T9336 T9331 auxpass were,normalized
R5870 T9337 T9331 prep according,normalized
R5871 T9338 T9337 prep to,according
R5872 T9339 T9340 poss their,activity
R5873 T9340 T9338 pobj activity,to
R5874 T9341 T9342 compound acetylcholine,esterase
R5875 T9342 T9340 compound esterase,activity
R5876 T9343 T9331 punct .,normalized
R5877 T9345 T9346 amod Human,cells
R5878 T9346 T9349 nsubjpass cells,used
R5879 T9347 T9346 amod red,cells
R5880 T9348 T9346 compound blood,cells
R5881 T9350 T9351 punct (,co
R5882 T9351 T9346 parataxis co,cells
R5883 T9352 T9351 punct ),co
R5884 T9353 T9349 auxpass were,used
R5885 T9354 T9349 prep for,used
R5886 T9355 T9354 pobj control,for
R5887 T9356 T9349 cc and,used
R5888 T9357 T9349 conj normalized,used
R5889 T9358 T9357 advmod independently,normalized
R5890 T9359 T9349 punct .,used
R5891 T9398 T9397 prep of,Association
R5892 T9399 T9400 compound annexin,A7
R5893 T9400 T9398 pobj A7,of
R5894 T9401 T9400 cc and,A7
R5895 T9402 T9400 conj sorcin,A7
R5896 T9403 T9397 prep with,Association
R5897 T9404 T9405 amod distinct,fractions
R5898 T9405 T9403 pobj fractions,with
R5899 T9406 T9407 amod red,cell
R5900 T9407 T9405 compound cell,fractions
R5901 T9408 T9407 compound blood,cell
R5902 T9409 T9410 compound plasma,membrane
R5903 T9410 T9405 compound membrane,fractions
R5904 T9411 T9397 punct .,Association
R5905 T9413 T9414 amod Lysed,cells
R5906 T9414 T9418 nsubjpass cells,added
R5907 T9415 T9414 amod human,cells
R5908 T9416 T9414 amod red,cells
R5909 T9417 T9414 compound blood,cells
R5910 T9419 T9418 auxpass were,added
R5911 T9420 T9418 prep to,added
R5912 T9421 T9422 det a,gradient
R5913 T9422 T9420 pobj gradient,to
R5914 T9423 T9424 npadvmod self,forming
R5915 T9424 T9422 amod forming,gradient
R5916 T9425 T9422 compound iodixanol,gradient
R5917 T9426 T9422 compound density,gradient
R5918 T9427 T9418 punct .,added
R5919 T9429 T9430 det The,density
R5920 T9430 T9431 nsubj density,increases
R5921 T9432 T9430 prep of,density
R5922 T9433 T9434 det the,gradient
R5923 T9434 T9432 pobj gradient,of
R5924 T9435 T9431 prep from,increases
R5925 T9436 T9435 pobj left,from
R5926 T9437 T9438 punct (,g
R5927 T9438 T9436 parataxis g,left
R5928 T9439 T9438 nummod 1.06,g
R5929 T9440 T9441 punct /,ml
R5930 T9441 T9438 prep ml,g
R5931 T9442 T9438 punct ),g
R5932 T9443 T9435 prep to,from
R5933 T9444 T9443 pobj right,to
R5934 T9445 T9446 punct (,g
R5935 T9446 T9444 parataxis g,right
R5936 T9447 T9446 nummod 1.20,g
R5937 T9448 T9449 punct /,ml
R5938 T9449 T9446 prep ml,g
R5939 T9450 T9446 punct ),g
R5940 T9451 T9431 punct .,increases
R5941 T9453 T9454 nsubjpass Fractions,analysed
R5942 T9455 T9454 auxpass were,analysed
R5943 T9456 T9454 prep by,analysed
R5944 T9457 T9456 pobj immunoblotting,by
R5945 T9458 T9454 advcl using,analysed
R5946 T9459 T9460 nmod mAb,203
R5947 T9460 T9458 dobj 203,using
R5948 T9461 T9462 punct –,217
R5949 T9462 T9460 prep 217,203
R5950 T9463 T9460 cc and,203
R5951 T9464 T9465 det the,antibody
R5952 T9465 T9460 conj antibody,203
R5953 T9466 T9465 nmod sorcin,antibody
R5954 T9467 T9465 amod polyclonal,antibody
R5955 T9468 T9454 punct .,analysed
R1408 T2601 T2599 pobj suggestion,to
R1409 T2602 T2601 punct ", ",suggestion
R1410 T2603 T2604 mark that,plays
R1411 T2604 T2601 acl plays,suggestion
R1412 T2605 T2604 punct ", ",plays
R1413 T2606 T2604 prep along,plays
R1414 T2607 T2606 prep with,along
R1415 T2608 T2609 compound raft,domains
R1416 T2609 T2607 pobj domains,with
R1417 T2610 T2604 punct ", ",plays
R1418 T2611 T2612 compound annexin,A7
R1419 T2612 T2604 nsubj A7,plays
R1582 T2787 T2773 prep for,both
R1583 T2788 T2787 pobj wt,for
R1584 T2789 T2788 cc and,wt
R1585 T2790 T2788 conj ko,wt
R1586 T2791 T2773 punct ),both
R1587 T2792 T2748 punct .,differ
R1588 T2794 T2795 nsubj It,appears
R1589 T2796 T2797 mark that,is
R1590 T2797 T2795 ccomp is,appears
R1591 T2798 T2799 compound annexin,A7
R1592 T2799 T2797 nsubj A7,is
R1593 T2800 T2797 punct ", ",is
R1594 T2801 T2802 mark although,described
R1595 T2802 T2797 advcl described,is
R1649 T2859 T2860 aux To,study
R1650 T2860 T2861 advcl study,used
R1651 T2862 T2863 det the,distribution
R1652 T2863 T2860 dobj distribution,study
R1653 T2864 T2863 prep of,distribution
R1654 T2865 T2866 compound annexin,A7
R1655 T2866 T2864 pobj A7,of
R1656 T2867 T2860 prep in,study
R1657 T2868 T2869 amod red,cells
R1658 T2869 T2867 pobj cells,in
R1659 T2870 T2869 compound blood,cells
R1660 T2871 T2861 nsubj we,used
R1661 T2872 T2873 npadvmod self,forming
R1662 T2873 T2874 amod forming,gradients
R1663 T2874 T2861 dobj gradients,used
R1328 T2512 T2513 det these,findings
R1329 T2513 T2509 dobj findings,extended
R1330 T2514 T2509 cc and,extended
R1331 T2515 T2509 conj show,extended
R1332 T2516 T2515 advmod here,show
R1333 T2517 T2518 mark that,is
R1334 T2518 T2515 ccomp is,show
R1402 T2594 T2595 poss Its,presence
R1403 T2595 T2596 nsubj presence,led
R1404 T2597 T2595 prep in,presence
R1405 T2598 T2597 pobj vesicles,in
R1406 T2599 T2596 prep to,led
R1407 T2600 T2601 det the,suggestion

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2247 0-10 PR:000004080 denotes Annexin A7
T2248 11-21 GO:0010467 denotes expression
T2249 25-40 CL:0000232 denotes red blood cells
T2250 29-34 UBERON:0000178 denotes blood
T2251 42-56 CL:0000232 denotes red blood cell
T2252 46-51 UBERON:0000178 denotes blood
T2253 65-76 GO:0070382 denotes exovesicles
T2254 84-93 CL:0000233 denotes platelets
T2255 153-160 SO:0001060 denotes isoform
T2256 164-174 PR:000004080 denotes annexin A7
T2257 191-197 PR:000015619 denotes sorcin
T2258 201-207 _FRAGMENT denotes micro-
T2259 216-224 GO:1990742 denotes vesicles
T2260 238-253 CL:0000232 denotes red blood cells
T2261 242-247 UBERON:0000178 denotes blood
T2262 315-329 GO:0045121 denotes membrane rafts
T2263 401-408 SO:0001060 denotes isoform
T2264 449-454 NCBITaxon:9606 denotes human
T2265 455-470 CL:0000232 denotes red blood cells
T2266 459-464 UBERON:0000178 denotes blood
T2267 489-497 SO:0001060 denotes isoforms
T2269 617-627 PR:000004080 denotes annexin A7
T2270 698-705 SO:0001060 denotes isoform
T2271 1142-1150 GO:0031982 denotes vesicles
T2272 1191-1203 GO:0045121 denotes raft domains
T2273 1205-1215 PR:000004080 denotes annexin A7
T2274 1232-1240 GO:0016020 denotes membrane
T2275 1262-1274 GO:0006900 denotes vesiculation
T2276 1328-1343 CL:0000232 denotes red blood cells
T2277 1332-1337 UBERON:0000178 denotes blood
T2278 1361-1371 PR:000004080 denotes annexin A7
T2279 1382-1386 NCBITaxon:10088 denotes mice
T2280 1388-1393 PR:000004080 denotes anxA7
T2282 1542-1547 NCBITaxon:9606 denotes human
T2283 1548-1553 UBERON:0000178 denotes blood
T2286 1624-1630 _FRAGMENT denotes micro-
T2287 1639-1647 GO:1990742 denotes vesicles
T2288 1669-1673 CHEBI:29108 denotes Ca2+
T2289 1674-1683 CHEBI:24869 denotes ionophore
T2290 1711-1724 CHEBI:15355 denotes acetylcholine
T2291 1772-1782 PR:000004080 denotes Annexin A7
T2292 1802-1809 GO:0031982 denotes vesicle
T2293 1849-1857 GO:0031982 denotes vesicles
T2294 1879-1886 SO:0001060 denotes isoform
T2295 1913-1921 GO:0031982 denotes vesicles
T2296 1944-1951 GO:0031982 denotes vesicle
T2297 2005-2019 CL:0000232 denotes red blood cell
T2298 2009-2014 UBERON:0000178 denotes blood
T2299 2020-2028 GO:0031982 denotes vesicles
T2300 2072-2080 GO:0031982 denotes vesicles
T2301 2094-2107 GO:1990742 denotes microvesicles
T2302 2153-2163 PR:000004080 denotes annexin A7
T2303 2204-2213 GO:0016020 denotes membranes
T2304 2273-2287 CL:0000232 denotes red blood cell
T2305 2277-2282 UBERON:0000178 denotes blood
T2306 2288-2299 GO:0070382 denotes exovesicles
T2307 2336-2342 PR:000015619 denotes sorcin
T2308 2367-2375 GO:0031982 denotes vesicles
T2309 2394-2400 PR:000015619 denotes sorcin
T2310 2439-2447 GO:0031982 denotes vesicles
T2311 3123-3133 PR:000004080 denotes annexin A7
T2312 3137-3152 CL:0000232 denotes red blood cells
T2313 3141-3146 UBERON:0000178 denotes blood
T2314 3174-3183 CHEBI:31705 denotes iodixanol
T2315 3208-3216 SO:0001060 denotes isoforms
T2316 3336-3345 GO:0016020 denotes membranes
T2317 3353-3362 GO:0016020 denotes membranes
T2318 3379-3395 GO:0005886 denotes plasma membranes
T2319 3399-3414 CL:0000232 denotes red blood cells
T2320 3403-3408 UBERON:0000178 denotes blood
T2321 3431-3441 GO:0043226 denotes organelles
T2322 3547-3557 PR:000004080 denotes annexin A7
T2323 3561-3580 GO:0044425 denotes membrane subdomains
T2324 3601-3606 CHEBI:18059 denotes lipid
T2325 3610-3615 CHEBI:18059 denotes lipid
T2326 3681-3687 PR:000015619 denotes sorcin
T2327 3778-3786 GO:0031982 denotes vesicles
T2328 3817-3827 PR:000004080 denotes annexin A7
T2329 3853-3862 CL:0000233 denotes platelets
T2330 3867-3877 PR:000004080 denotes annexin A7
T2331 3878-3888 GO:0010467 denotes expression
T2332 3913-3920 SO:0001060 denotes isoform
T9053 752-762 GO:0010467 denotes Expression
T9054 766-776 PR:000004080 denotes annexin A7
T9055 780-785 NCBITaxon:9606 denotes human
T9056 786-801 CL:0000232 denotes red blood cells
T9057 790-795 UBERON:0000178 denotes blood
T9058 810-815 NCBITaxon:9606 denotes human
T9059 816-825 CL:0000233 denotes platelets
T9060 869-872 CHEBI:8984 denotes SDS
T9061 884-894 CHEBI:28619 denotes acrylamide
T9062 997-1004 MOP:0000779 denotes coupled
T9063 1005-1013 GO:0042571 denotes antibody
T9064 1036-1044 SO:0001060 denotes isoforms
T9065 1061-1076 CL:0000232 denotes red blood cells
T9066 1065-1070 UBERON:0000178 denotes blood
T9067 1081-1090 CL:0000233 denotes platelets
T9068 1106-1113 SO:0001060 denotes isoform
T9188 2482-2492 PR:000004080 denotes annexin A7
T9189 2497-2503 PR:000015619 denotes sorcin
T9190 2507-2518 GO:0070382 denotes exovesicles
T9191 2532-2547 CL:0000232 denotes red blood cells
T9192 2536-2541 UBERON:0000178 denotes blood
T9193 2549-2557 GO:0031982 denotes Vesicles
T9194 2582-2587 PR:000004080 denotes anxA7
T9195 2623-2627 CHEBI:29108 denotes Ca2+
T9196 2628-2637 CHEBI:24869 denotes ionophore
T9197 2743-2749 PR:000015619 denotes sorcin
T9198 2761-2769 GO:0042571 denotes antibody
T9199 2783-2793 PR:000004080 denotes annexin A7
T9200 2798-2804 PR:000015619 denotes sorcin
T9201 2830-2838 GO:0031982 denotes vesicles
T9202 2851-2858 SO:0001060 denotes isoform
T9203 2862-2872 PR:000004080 denotes annexin A7
T9204 2906-2914 GO:0031982 denotes vesicles
T9205 2932-2940 GO:0031982 denotes vesicles
T9206 2982-2995 CHEBI:15355 denotes acetylcholine
T9207 3015-3020 NCBITaxon:9606 denotes Human
T9208 3021-3036 CL:0000232 denotes red blood cells
T9209 3025-3030 UBERON:0000178 denotes blood
T9371 3956-3966 PR:000004080 denotes annexin A7
T9372 3971-3977 PR:000015619 denotes sorcin
T9373 3992-4006 CL:0000232 denotes red blood cell
T9374 3996-4001 UBERON:0000178 denotes blood
T9375 4007-4022 GO:0005886 denotes plasma membrane
T9376 4034-4039 GO:0019835 denotes Lysed
T9377 4040-4045 NCBITaxon:9606 denotes human
T9378 4046-4061 CL:0000232 denotes red blood cells
T9379 4050-4055 UBERON:0000178 denotes blood
T9380 4091-4100 CHEBI:31705 denotes iodixanol
T9381 4269-4275 PR:000015619 denotes sorcin
T9382 4287-4295 GO:0042571 denotes antibody
T2268 551-558 SO:0001060 denotes isoform
T2281 1406-1417 GO:0070382 denotes exovesicles
T2284 1586-1596 PR:000004080 denotes annexin A7
T2285 1611-1622 GO:0070382 denotes exovesicles
R1260 T2259 T2258 _lexicallyChainedTo vesicles,micro-
R1261 T2287 T2286 _lexicallyChainedTo vesicles,micro-

2_test

Id Subject Object Predicate Lexical cue
12925238-11895795-8102397 331-332 11895795 denotes 7
12925238-11895795-8102398 3667-3668 11895795 denotes 7

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2333 0-10 PR_EXT:000004080 denotes Annexin A7
T2334 11-21 GO:0010467 denotes expression
T2335 25-40 CL:0000232 denotes red blood cells
T2336 29-34 UBERON:0000178 denotes blood
T2337 35-40 CL_GO_EXT:cell denotes cells
T2338 42-56 CL:0000232 denotes red blood cell
T2339 46-51 UBERON:0000178 denotes blood
T2340 52-56 CL_GO_EXT:cell denotes cell
T2341 65-76 GO:0070382 denotes exovesicles
T2342 84-93 CL:0000233 denotes platelets
T2343 153-160 SO_EXT:0001060 denotes isoform
T2344 164-174 PR_EXT:000004080 denotes annexin A7
T2345 191-197 PR_EXT:000015619 denotes sorcin
T2346 201-207 _FRAGMENT denotes micro-
T2347 216-224 GO:1990742 denotes vesicles
T2348 238-253 CL:0000232 denotes red blood cells
T2349 242-247 UBERON:0000178 denotes blood
T2350 248-253 CL_GO_EXT:cell denotes cells
T2351 284-289 GO_UBERON_EXT:lumen denotes lumen
T2352 315-329 GO:0045121 denotes membrane rafts
T2353 401-408 SO_EXT:0001060 denotes isoform
T2354 449-454 NCBITaxon:9606 denotes human
T2355 455-470 CL:0000232 denotes red blood cells
T2356 459-464 UBERON:0000178 denotes blood
T2357 465-470 CL_GO_EXT:cell denotes cells
T2358 489-497 SO_EXT:0001060 denotes isoforms
T2359 551-558 SO_EXT:0001060 denotes isoform
T2360 575-581 CHEBI_EXT:silver denotes silver
T2361 617-627 PR_EXT:000004080 denotes annexin A7
T2395 1981-1990 SO_EXT:wild_type_entity_or_quality denotes wild type
T2397 2009-2014 UBERON:0000178 denotes blood
T2398 2015-2019 CL_GO_EXT:cell denotes cell
T2399 2020-2028 GO:0031982 denotes vesicles
T2400 2050-2054 GO_PR_EXT:acetylcholinesterase denotes AChE
T2401 2072-2080 GO:0031982 denotes vesicles
T2402 2094-2107 GO:1990742 denotes microvesicles
T2403 2125-2127 SO_EXT:wild_type_entity denotes wt
T2404 2153-2163 PR_EXT:000004080 denotes annexin A7
T2405 2204-2213 GO:0016020 denotes membranes
T2406 2273-2287 CL:0000232 denotes red blood cell
T2407 2277-2282 UBERON:0000178 denotes blood
T2408 2283-2287 CL_GO_EXT:cell denotes cell
T2409 2288-2299 GO:0070382 denotes exovesicles
T2410 2336-2342 PR_EXT:000015619 denotes sorcin
T2411 2346-2355 SO_EXT:wild_type_entity_or_quality denotes wild type
T2412 2360-2366 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T2413 2367-2375 GO:0031982 denotes vesicles
T2414 2394-2400 PR_EXT:000015619 denotes sorcin
T2415 2428-2434 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T2416 2439-2447 GO:0031982 denotes vesicles
T9086 1071-1076 CL_GO_EXT:cell denotes cells
T9087 1081-1090 CL:0000233 denotes platelets
T9088 1106-1113 SO_EXT:0001060 denotes isoform
T9210 2482-2492 PR_EXT:000004080 denotes annexin A7
T9211 2497-2503 PR_EXT:000015619 denotes sorcin
T9212 2507-2518 GO:0070382 denotes exovesicles
T9213 2532-2547 CL:0000232 denotes red blood cells
T9214 2536-2541 UBERON:0000178 denotes blood
T9215 2542-2547 CL_GO_EXT:cell denotes cells
T9216 2549-2557 GO:0031982 denotes Vesicles
T9217 2563-2572 SO_EXT:wild_type_entity denotes wild type
T9218 2574-2576 SO_EXT:wild_type_entity denotes wt
T9219 2582-2587 PR_EXT:000004080 denotes anxA7
T9220 2587-2588 SO_EXT:sequence_nullness_or_absence denotes -
T9221 2589-2590 SO_EXT:sequence_nullness_or_absence denotes -
T9222 2591-2597 SO_EXT:sequence_altered_entity denotes mutant
T9223 2623-2627 CHEBI:29108 denotes Ca2+
T9224 2628-2637 CHEBI_EXT:24869 denotes ionophore
T9225 2743-2749 PR_EXT:000015619 denotes sorcin
T9226 2761-2769 GO:0042571 denotes antibody
T9227 2783-2793 PR_EXT:000004080 denotes annexin A7
T9228 2798-2804 PR_EXT:000015619 denotes sorcin
T9229 2830-2838 GO:0031982 denotes vesicles
T9230 2851-2858 SO_EXT:0001060 denotes isoform
T9231 2862-2872 PR_EXT:000004080 denotes annexin A7
T9232 2906-2914 GO:0031982 denotes vesicles
T9233 2932-2940 GO:0031982 denotes vesicles
T9234 2982-2995 CHEBI:15355 denotes acetylcholine
T9235 2982-3004 GO_PR_EXT:acetylcholinesterase denotes acetylcholine esterase
T9236 3015-3020 NCBITaxon:9606 denotes Human
T9237 3021-3036 CL:0000232 denotes red blood cells
T9238 3025-3030 UBERON:0000178 denotes blood
T9239 3031-3036 CL_GO_EXT:cell denotes cells
T9391 4046-4061 CL:0000232 denotes red blood cells
T9392 4050-4055 UBERON:0000178 denotes blood
T9393 4056-4061 CL_GO_EXT:cell denotes cells
T9394 4091-4100 CHEBI:31705 denotes iodixanol
T9395 4269-4275 PR_EXT:000015619 denotes sorcin
T9396 4287-4295 GO:0042571 denotes antibody
T9069 752-762 GO:0010467 denotes Expression
T9070 766-776 PR_EXT:000004080 denotes annexin A7
T9071 780-785 NCBITaxon:9606 denotes human
T9072 786-801 CL:0000232 denotes red blood cells
T9073 790-795 UBERON:0000178 denotes blood
T9074 796-801 CL_GO_EXT:cell denotes cells
T9075 810-815 NCBITaxon:9606 denotes human
T9076 816-825 CL:0000233 denotes platelets
T9077 831-838 CHEBI_PR_EXT:protein denotes Protein
T9078 869-872 CHEBI:8984 denotes SDS
T9079 884-894 CHEBI:28619 denotes acrylamide
T9080 986-996 GO_EXT:0004601 denotes peroxidase
T9081 997-1004 MOP:0000779 denotes coupled
T9082 1005-1013 GO:0042571 denotes antibody
T9083 1036-1044 SO_EXT:0001060 denotes isoforms
T9084 1061-1076 CL:0000232 denotes red blood cells
T9085 1065-1070 UBERON:0000178 denotes blood
T9383 3956-3966 PR_EXT:000004080 denotes annexin A7
T9384 3971-3977 PR_EXT:000015619 denotes sorcin
T9385 3992-4006 CL:0000232 denotes red blood cell
T9386 3996-4001 UBERON:0000178 denotes blood
T9387 4002-4006 CL_GO_EXT:cell denotes cell
T9388 4007-4022 GO:0005886 denotes plasma membrane
T9389 4034-4039 GO:0019835 denotes Lysed
T9390 4040-4045 NCBITaxon:9606 denotes human
T2362 641-648 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T2363 698-705 SO_EXT:0001060 denotes isoform
T2364 1142-1150 GO:0031982 denotes vesicles
T2365 1191-1203 GO:0045121 denotes raft domains
T2366 1205-1215 PR_EXT:000004080 denotes annexin A7
T2367 1232-1240 GO:0016020 denotes membrane
T2368 1262-1274 GO:0006900 denotes vesiculation
T2369 1328-1343 CL:0000232 denotes red blood cells
T2370 1332-1337 UBERON:0000178 denotes blood
T2371 1338-1343 CL_GO_EXT:cell denotes cells
T2372 1361-1371 PR_EXT:000004080 denotes annexin A7
T2373 1382-1386 NCBITaxon:10088 denotes mice
T2374 1388-1393 PR_EXT:000004080 denotes anxA7
T2375 1393-1394 SO_EXT:sequence_nullness_or_absence denotes -
T2376 1395-1396 SO_EXT:sequence_nullness_or_absence denotes -
T2377 1406-1417 GO:0070382 denotes exovesicles
T2378 1542-1547 NCBITaxon:9606 denotes human
T2379 1548-1553 UBERON:0000178 denotes blood
T2380 1586-1596 PR_EXT:000004080 denotes annexin A7
T2381 1611-1622 GO:0070382 denotes exovesicles
T2382 1624-1630 _FRAGMENT denotes micro-
T2383 1639-1647 GO:1990742 denotes vesicles
T2384 1654-1662 GO_EXT:biological_release denotes released
T2385 1669-1673 CHEBI:29108 denotes Ca2+
T2386 1674-1683 CHEBI_EXT:24869 denotes ionophore
T2387 1711-1724 CHEBI:15355 denotes acetylcholine
T2388 1711-1733 GO_PR_EXT:acetylcholinesterase denotes acetylcholine esterase
T2389 1772-1782 PR_EXT:000004080 denotes Annexin A7
T2390 1802-1809 GO:0031982 denotes vesicle
T2391 1849-1857 GO:0031982 denotes vesicles
T2392 1879-1886 SO_EXT:0001060 denotes isoform
T2393 1913-1921 GO:0031982 denotes vesicles
T2394 1944-1951 GO:0031982 denotes vesicle
T2396 2005-2019 CL:0000232 denotes red blood cell
T2417 3123-3133 PR_EXT:000004080 denotes annexin A7
T2418 3137-3152 CL:0000232 denotes red blood cells
T2419 3141-3146 UBERON:0000178 denotes blood
T2420 3147-3152 CL_GO_EXT:cell denotes cells
T2421 3174-3183 CHEBI:31705 denotes iodixanol
T2422 3208-3216 SO_EXT:0001060 denotes isoforms
T2423 3336-3345 GO:0016020 denotes membranes
T2424 3353-3362 GO:0016020 denotes membranes
T2425 3379-3395 GO:0005886 denotes plasma membranes
T2426 3399-3414 CL:0000232 denotes red blood cells
T2427 3403-3408 UBERON:0000178 denotes blood
T2428 3409-3414 CL_GO_EXT:cell denotes cells
T2429 3431-3441 GO:0043226 denotes organelles
T2430 3536-3543 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T2431 3547-3557 PR_EXT:000004080 denotes annexin A7
T2432 3561-3580 GO:0044425 denotes membrane subdomains
T2433 3601-3606 CHEBI:18059 denotes lipid
T2434 3610-3615 CHEBI:18059 denotes lipid
T2435 3616-3623 CHEBI_PR_EXT:protein denotes protein
T2436 3681-3687 PR_EXT:000015619 denotes sorcin
T2437 3778-3786 GO:0031982 denotes vesicles
T2438 3817-3827 PR_EXT:000004080 denotes annexin A7
T2439 3853-3862 CL:0000233 denotes platelets
T2440 3867-3877 PR_EXT:000004080 denotes annexin A7
T2441 3878-3888 GO:0010467 denotes expression
T2442 3913-3920 SO_EXT:0001060 denotes isoform
R1262 T2347 T2346 _lexicallyChainedTo vesicles,micro-
R1263 T2383 T2382 _lexicallyChainedTo vesicles,micro-