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methods\nFor standard haematopoetic measurements and cell counts on murine and human blood samples about 300 μl of blood were analysed with an ADVIA 120 electronic cell counter (Bayer). With this equipment red blood cells and platelets are \"transformed\" into spherical bodies without changing their volume. Therefore number and volume of red blood cells and platelets are not calculated values, but directly measured values. The following data were statistically analysed: leucocytes, red blood cells, haemoglobin, haematocrit, mean cellular volume, mean cellular haemoglobin, mean cellular haemoglobin concentration, platelet number, neutrophiles, lymphocytes, monocytes, eosinophiles, basophiles.\nPeptide mass fingerprinting was performed on a Bruker Reflex IV MALDI-TOF mass spectrometer. Recombinant annexin A7 was purified according to [27], F-actin-binding of recombinant annexin A7 was done as described [28]. SDS-PAGE and western blotting were done as described [29,30]. Detection in immunoblots was with enhanced chemiluminescence [17]. Antibodies employed were mab 203–217 directed against mouse annexin A7 core domain [5] and a polyclonal rabbit anti-sorcin antibody [31].\nFor statistical analyses the following tests were performed: David et al. range test to ensure samples are from a normal distribution, F-test looking for a samples' homoscedascity, Student's T-test to judge if samples of different groups have a different mean value and are parts from different normal distributions. The exact probability values and the significance of an analysis are indicated when the experiments are described."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T8657","span":{"begin":35,"end":48},"obj":"GO:0030097"},{"id":"T8658","span":{"begin":81,"end":87},"obj":"NCBITaxon:39107"},{"id":"T8659","span":{"begin":92,"end":97},"obj":"NCBITaxon:9606"},{"id":"T8660","span":{"begin":98,"end":103},"obj":"UBERON:0000178"},{"id":"T8661","span":{"begin":128,"end":133},"obj":"UBERON:0000178"},{"id":"T8662","span":{"begin":219,"end":234},"obj":"CL:0000232"},{"id":"T8663","span":{"begin":223,"end":228},"obj":"UBERON:0000178"},{"id":"T8664","span":{"begin":239,"end":248},"obj":"CL:0000233"},{"id":"T8665","span":{"begin":351,"end":366},"obj":"CL:0000232"},{"id":"T8666","span":{"begin":355,"end":360},"obj":"UBERON:0000178"},{"id":"T8667","span":{"begin":371,"end":380},"obj":"CL:0000233"},{"id":"T8668","span":{"begin":486,"end":496},"obj":"CL:0000738"},{"id":"T8669","span":{"begin":498,"end":513},"obj":"CL:0000232"},{"id":"T8670","span":{"begin":502,"end":507},"obj":"UBERON:0000178"},{"id":"T8671","span":{"begin":631,"end":639},"obj":"CL:0000233"},{"id":"T8672","span":{"begin":648,"end":660},"obj":"CL:0000775"},{"id":"T8673","span":{"begin":662,"end":673},"obj":"CL:0000542"},{"id":"T8674","span":{"begin":675,"end":684},"obj":"CL:0000576"},{"id":"T8675","span":{"begin":686,"end":698},"obj":"CL:0000771"},{"id":"T8676","span":{"begin":700,"end":710},"obj":"CL:0000767"},{"id":"T8677","span":{"begin":817,"end":827},"obj":"PR:000004080"},{"id":"T8678","span":{"begin":860,"end":867},"obj":"GO:0031941"},{"id":"T8679","span":{"begin":891,"end":901},"obj":"PR:000004080"},{"id":"T8680","span":{"begin":930,"end":933},"obj":"CHEBI:8984"},{"id":"T8681","span":{"begin":1059,"end":1069},"obj":"GO:0042571"},{"id":"T8682","span":{"begin":1113,"end":1118},"obj":"NCBITaxon:10088"},{"id":"T8683","span":{"begin":1119,"end":1129},"obj":"PR:000004080"},{"id":"T8684","span":{"begin":1135,"end":1141},"obj":"SO:0000417"},{"id":"T8685","span":{"begin":1163,"end":1169},"obj":"NCBITaxon:9986"},{"id":"T8686","span":{"begin":1175,"end":1181},"obj":"PR:000015619"},{"id":"T8687","span":{"begin":1182,"end":1190},"obj":"GO:0042571"}],"text":"Miscellaneous methods\nFor standard haematopoetic measurements and cell counts on murine and human blood samples about 300 μl of blood were analysed with an ADVIA 120 electronic cell counter (Bayer). With this equipment red blood cells and platelets are \"transformed\" into spherical bodies without changing their volume. Therefore number and volume of red blood cells and platelets are not calculated values, but directly measured values. The following data were statistically analysed: leucocytes, red blood cells, haemoglobin, haematocrit, mean cellular volume, mean cellular haemoglobin, mean cellular haemoglobin concentration, platelet number, neutrophiles, lymphocytes, monocytes, eosinophiles, basophiles.\nPeptide mass fingerprinting was performed on a Bruker Reflex IV MALDI-TOF mass spectrometer. Recombinant annexin A7 was purified according to [27], F-actin-binding of recombinant annexin A7 was done as described [28]. SDS-PAGE and western blotting were done as described [29,30]. Detection in immunoblots was with enhanced chemiluminescence [17]. Antibodies employed were mab 203–217 directed against mouse annexin A7 core domain [5] and a polyclonal rabbit anti-sorcin antibody [31].\nFor statistical analyses the following tests were performed: David et al. range test to ensure samples are from a normal distribution, F-test looking for a samples' homoscedascity, Student's T-test to judge if samples of different groups have a different mean value and are parts from different normal distributions. The exact probability values and the significance of an analysis are indicated when the experiments are described."}

{"project":"2_test","denotations":[{"id":"12925238-9231902-8102429","span":{"begin":855,"end":857},"obj":"9231902"},{"id":"12925238-12374734-8102430","span":{"begin":925,"end":927},"obj":"12374734"},{"id":"12925238-5432063-8102431","span":{"begin":984,"end":986},"obj":"5432063"},{"id":"12925238-388439-8102432","span":{"begin":987,"end":989},"obj":"388439"},{"id":"12925238-11390641-8102433","span":{"begin":1054,"end":1056},"obj":"11390641"},{"id":"12925238-7738119-8102434","span":{"begin":1143,"end":1144},"obj":"7738119"},{"id":"12925238-10651630-8102435","span":{"begin":1192,"end":1194},"obj":"10651630"}],"text":"Miscellaneous methods\nFor standard haematopoetic measurements and cell counts on murine and human blood samples about 300 μl of blood were analysed with an ADVIA 120 electronic cell counter (Bayer). With this equipment red blood cells and platelets are \"transformed\" into spherical bodies without changing their volume. Therefore number and volume of red blood cells and platelets are not calculated values, but directly measured values. The following data were statistically analysed: leucocytes, red blood cells, haemoglobin, haematocrit, mean cellular volume, mean cellular haemoglobin, mean cellular haemoglobin concentration, platelet number, neutrophiles, lymphocytes, monocytes, eosinophiles, basophiles.\nPeptide mass fingerprinting was performed on a Bruker Reflex IV MALDI-TOF mass spectrometer. Recombinant annexin A7 was purified according to [27], F-actin-binding of recombinant annexin A7 was done as described [28]. SDS-PAGE and western blotting were done as described [29,30]. Detection in immunoblots was with enhanced chemiluminescence [17]. Antibodies employed were mab 203–217 directed against mouse annexin A7 core domain [5] and a polyclonal rabbit anti-sorcin antibody [31].\nFor statistical analyses the following tests were performed: David et al. range test to ensure samples are from a normal distribution, F-test looking for a samples' homoscedascity, Student's T-test to judge if samples of different groups have a different mean value and are parts from different normal distributions. The exact probability values and the significance of an analysis are indicated when the experiments are described."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T8688","span":{"begin":35,"end":48},"obj":"GO:0030097"},{"id":"T8689","span":{"begin":66,"end":70},"obj":"CL_GO_EXT:cell"},{"id":"T8690","span":{"begin":81,"end":87},"obj":"NCBITaxon:39107"},{"id":"T8691","span":{"begin":92,"end":97},"obj":"NCBITaxon:9606"},{"id":"T8692","span":{"begin":98,"end":103},"obj":"UBERON:0000178"},{"id":"T8693","span":{"begin":128,"end":133},"obj":"UBERON:0000178"},{"id":"T8694","span":{"begin":177,"end":181},"obj":"CL_GO_EXT:cell"},{"id":"T8695","span":{"begin":219,"end":234},"obj":"CL:0000232"},{"id":"T8696","span":{"begin":223,"end":228},"obj":"UBERON:0000178"},{"id":"T8697","span":{"begin":229,"end":234},"obj":"CL_GO_EXT:cell"},{"id":"T8698","span":{"begin":239,"end":248},"obj":"CL:0000233"},{"id":"T8699","span":{"begin":351,"end":366},"obj":"CL:0000232"},{"id":"T8700","span":{"begin":355,"end":360},"obj":"UBERON:0000178"},{"id":"T8701","span":{"begin":361,"end":366},"obj":"CL_GO_EXT:cell"},{"id":"T8702","span":{"begin":371,"end":380},"obj":"CL:0000233"},{"id":"T8703","span":{"begin":486,"end":496},"obj":"CL:0000738"},{"id":"T8704","span":{"begin":498,"end":513},"obj":"CL:0000232"},{"id":"T8705","span":{"begin":502,"end":507},"obj":"UBERON:0000178"},{"id":"T8706","span":{"begin":508,"end":513},"obj":"CL_GO_EXT:cell"},{"id":"T8707","span":{"begin":515,"end":526},"obj":"CHEBI_GO_EXT:hemoglobin"},{"id":"T8708","span":{"begin":546,"end":554},"obj":"CL_GO_EXT:cell"},{"id":"T8709","span":{"begin":568,"end":576},"obj":"CL_GO_EXT:cell"},{"id":"T8710","span":{"begin":577,"end":588},"obj":"CHEBI_GO_EXT:hemoglobin"},{"id":"T8711","span":{"begin":595,"end":603},"obj":"CL_GO_EXT:cell"},{"id":"T8712","span":{"begin":604,"end":615},"obj":"CHEBI_GO_EXT:hemoglobin"},{"id":"T8713","span":{"begin":631,"end":639},"obj":"CL:0000233"},{"id":"T8714","span":{"begin":648,"end":660},"obj":"CL:0000775"},{"id":"T8715","span":{"begin":662,"end":673},"obj":"CL:0000542"},{"id":"T8716","span":{"begin":675,"end":684},"obj":"CL:0000576"},{"id":"T8717","span":{"begin":686,"end":698},"obj":"CL:0000771"},{"id":"T8718","span":{"begin":700,"end":710},"obj":"CL:0000767"},{"id":"T8719","span":{"begin":712,"end":719},"obj":"CHEBI_SO_EXT:peptide_or_peptide_region"},{"id":"T8720","span":{"begin":805,"end":816},"obj":"GO_SO_EXT:sequence_rearrangement_process"},{"id":"T8721","span":{"begin":817,"end":827},"obj":"PR_EXT:000004080"},{"id":"T8722","span":{"begin":860,"end":867},"obj":"GO:0031941"},{"id":"T8723","span":{"begin":868,"end":875},"obj":"CHEMINF_GO_EXT:chemical_binding_or_bond_formation"},{"id":"T8724","span":{"begin":879,"end":890},"obj":"GO_SO_EXT:sequence_rearrangement_process"},{"id":"T8725","span":{"begin":891,"end":901},"obj":"PR_EXT:000004080"},{"id":"T8726","span":{"begin":930,"end":933},"obj":"CHEBI:8984"},{"id":"T8727","span":{"begin":1059,"end":1069},"obj":"GO:0042571"},{"id":"T8728","span":{"begin":1096,"end":1104},"obj":"GO_EXT:biological_direction_or_guidance"},{"id":"T8729","span":{"begin":1113,"end":1118},"obj":"NCBITaxon:10088"},{"id":"T8730","span":{"begin":1119,"end":1129},"obj":"PR_EXT:000004080"},{"id":"T8731","span":{"begin":1135,"end":1141},"obj":"SO_EXT:0000417"},{"id":"T8732","span":{"begin":1163,"end":1169},"obj":"NCBITaxon:9986"},{"id":"T8733","span":{"begin":1175,"end":1181},"obj":"PR_EXT:000015619"},{"id":"T8734","span":{"begin":1182,"end":1190},"obj":"GO:0042571"}],"text":"Miscellaneous methods\nFor standard haematopoetic measurements and cell counts on murine and human blood samples about 300 μl of blood were analysed with an ADVIA 120 electronic cell counter (Bayer). With this equipment red blood cells and platelets are \"transformed\" into spherical bodies without changing their volume. Therefore number and volume of red blood cells and platelets are not calculated values, but directly measured values. The following data were statistically analysed: leucocytes, red blood cells, haemoglobin, haematocrit, mean cellular volume, mean cellular haemoglobin, mean cellular haemoglobin concentration, platelet number, neutrophiles, lymphocytes, monocytes, eosinophiles, basophiles.\nPeptide mass fingerprinting was performed on a Bruker Reflex IV MALDI-TOF mass spectrometer. Recombinant annexin A7 was purified according to [27], F-actin-binding of recombinant annexin A7 was done as described [28]. SDS-PAGE and western blotting were done as described [29,30]. Detection in immunoblots was with enhanced chemiluminescence [17]. Antibodies employed were mab 203–217 directed against mouse annexin A7 core domain [5] and a polyclonal rabbit anti-sorcin antibody [31].\nFor statistical analyses the following tests were performed: David et al. range test to ensure samples are from a normal distribution, F-test looking for a samples' homoscedascity, Student's T-test to judge if samples of different groups have a different mean value and are parts from different normal distributions. The exact probability values and the significance of an analysis are indicated when the experiments are described."}