Id |
Subject |
Object |
Predicate |
Lexical cue |
T8326 |
0-8 |
NN |
denotes |
Platelet |
T8327 |
9-20 |
NN |
denotes |
aggregation |
T8328 |
21-26 |
NN |
denotes |
assay |
T8329 |
26-107 |
sentence |
denotes |
Approximately 500 μl of murine blood were centrifuged at 200 × g for 15 minutes. |
T8330 |
27-40 |
RB |
denotes |
Approximately |
T8331 |
41-44 |
CD |
denotes |
500 |
T8332 |
45-47 |
NNS |
denotes |
μl |
T8333 |
69-80 |
VBN |
denotes |
centrifuged |
T8334 |
48-50 |
IN |
denotes |
of |
T8335 |
51-57 |
JJ |
denotes |
murine |
T8336 |
58-63 |
NN |
denotes |
blood |
T8337 |
64-68 |
VBD |
denotes |
were |
T8338 |
81-83 |
IN |
denotes |
at |
T8339 |
84-87 |
CD |
denotes |
200 |
T8340 |
90-91 |
NN |
denotes |
g |
T8341 |
88-89 |
SYM |
denotes |
× |
T8342 |
92-95 |
IN |
denotes |
for |
T8343 |
96-98 |
CD |
denotes |
15 |
T8344 |
99-106 |
NNS |
denotes |
minutes |
T8345 |
106-107 |
. |
denotes |
. |
T8346 |
107-204 |
sentence |
denotes |
The resulting supernatants were transferred to new tubes and used as platelet rich plasma (PRP). |
T8347 |
108-111 |
DT |
denotes |
The |
T8348 |
122-134 |
NNS |
denotes |
supernatants |
T8349 |
112-121 |
VBG |
denotes |
resulting |
T8350 |
140-151 |
VBN |
denotes |
transferred |
T8351 |
135-139 |
VBD |
denotes |
were |
T8352 |
152-154 |
IN |
denotes |
to |
T8353 |
155-158 |
JJ |
denotes |
new |
T8354 |
159-164 |
NNS |
denotes |
tubes |
T8355 |
165-168 |
CC |
denotes |
and |
T8356 |
169-173 |
VBN |
denotes |
used |
T8357 |
174-176 |
IN |
denotes |
as |
T8358 |
177-185 |
NN |
denotes |
platelet |
T8359 |
186-190 |
JJ |
denotes |
rich |
T8360 |
191-197 |
NN |
denotes |
plasma |
T8361 |
198-199 |
-LRB- |
denotes |
( |
T8362 |
199-202 |
NN |
denotes |
PRP |
T8363 |
202-203 |
-RRB- |
denotes |
) |
T8364 |
203-204 |
. |
denotes |
. |
T8365 |
204-397 |
sentence |
denotes |
The platelet aggregation measurements were done according to the method developed by Born and Cross [26] using an APACT photometer (Labor, Ahrendsburg, FRG) and the APACT software (APACT 1.4). |
T8366 |
205-208 |
DT |
denotes |
The |
T8367 |
230-242 |
NNS |
denotes |
measurements |
T8368 |
209-217 |
NN |
denotes |
platelet |
T8369 |
218-229 |
NN |
denotes |
aggregation |
T8370 |
248-252 |
VBN |
denotes |
done |
T8371 |
243-247 |
VBD |
denotes |
were |
T8372 |
253-262 |
VBG |
denotes |
according |
T8373 |
263-265 |
IN |
denotes |
to |
T8374 |
266-269 |
DT |
denotes |
the |
T8375 |
270-276 |
NN |
denotes |
method |
T8376 |
277-286 |
VBN |
denotes |
developed |
T8377 |
287-289 |
IN |
denotes |
by |
T8378 |
290-294 |
NNP |
denotes |
Born |
T8379 |
295-298 |
CC |
denotes |
and |
T8380 |
299-304 |
NNP |
denotes |
Cross |
T8381 |
305-306 |
-LRB- |
denotes |
[ |
T8382 |
306-308 |
CD |
denotes |
26 |
T8383 |
308-309 |
-RRB- |
denotes |
] |
T8384 |
310-315 |
VBG |
denotes |
using |
T8385 |
316-318 |
DT |
denotes |
an |
T8386 |
325-335 |
NN |
denotes |
photometer |
T8387 |
319-324 |
NN |
denotes |
APACT |
T8388 |
336-337 |
-LRB- |
denotes |
( |
T8389 |
357-360 |
NN |
denotes |
FRG |
T8390 |
337-342 |
NN |
denotes |
Labor |
T8391 |
342-344 |
, |
denotes |
, |
T8392 |
344-355 |
NN |
denotes |
Ahrendsburg |
T8393 |
355-357 |
, |
denotes |
, |
T8394 |
360-361 |
-RRB- |
denotes |
) |
T8395 |
362-365 |
CC |
denotes |
and |
T8396 |
366-369 |
DT |
denotes |
the |
T8397 |
376-384 |
NN |
denotes |
software |
T8398 |
370-375 |
NN |
denotes |
APACT |
T8399 |
385-386 |
-LRB- |
denotes |
( |
T8400 |
386-391 |
NN |
denotes |
APACT |
T8401 |
392-395 |
CD |
denotes |
1.4 |
T8402 |
395-396 |
-RRB- |
denotes |
) |
T8403 |
396-397 |
. |
denotes |
. |
T8404 |
397-514 |
sentence |
denotes |
Subsequently samples of the PRP were adjusted with buffer (5 mM Hepes, 150 mM NaCl, pH 7.3) to 250,000 platelets/μl. |
T8405 |
398-410 |
RB |
denotes |
Subsequently |
T8406 |
435-443 |
VBN |
denotes |
adjusted |
T8407 |
411-418 |
NNS |
denotes |
samples |
T8408 |
419-421 |
IN |
denotes |
of |
T8409 |
422-425 |
DT |
denotes |
the |
T8410 |
426-429 |
NN |
denotes |
PRP |
T8411 |
430-434 |
VBD |
denotes |
were |
T8412 |
444-448 |
IN |
denotes |
with |
T8413 |
449-455 |
NN |
denotes |
buffer |
T8414 |
456-457 |
-LRB- |
denotes |
( |
T8415 |
482-484 |
NN |
denotes |
pH |
T8416 |
457-458 |
CD |
denotes |
5 |
T8417 |
459-461 |
NN |
denotes |
mM |
T8418 |
462-467 |
NN |
denotes |
Hepes |
T8419 |
467-469 |
, |
denotes |
, |
T8420 |
469-472 |
CD |
denotes |
150 |
T8421 |
473-475 |
NN |
denotes |
mM |
T8422 |
476-480 |
NN |
denotes |
NaCl |
T8423 |
480-482 |
, |
denotes |
, |
T8424 |
485-488 |
CD |
denotes |
7.3 |
T8425 |
488-489 |
-RRB- |
denotes |
) |
T8426 |
490-492 |
IN |
denotes |
to |
T8427 |
493-500 |
CD |
denotes |
250,000 |
T8428 |
501-510 |
NNS |
denotes |
platelets |
T8429 |
510-511 |
SYM |
denotes |
/ |
T8430 |
511-513 |
NN |
denotes |
μl |
T8431 |
513-514 |
. |
denotes |
. |
T8432 |
514-639 |
sentence |
denotes |
A constant platelet count was used throughout allowing comparisons between wild type and knock out murine and human samples. |
T8433 |
515-516 |
DT |
denotes |
A |
T8434 |
535-540 |
NN |
denotes |
count |
T8435 |
517-525 |
JJ |
denotes |
constant |
T8436 |
526-534 |
NN |
denotes |
platelet |
T8437 |
545-549 |
VBN |
denotes |
used |
T8438 |
541-544 |
VBD |
denotes |
was |
T8439 |
550-560 |
RB |
denotes |
throughout |
T8440 |
561-569 |
VBG |
denotes |
allowing |
T8441 |
570-581 |
NNS |
denotes |
comparisons |
T8442 |
582-589 |
IN |
denotes |
between |
T8443 |
590-594 |
JJ |
denotes |
wild |
T8444 |
595-599 |
NN |
denotes |
type |
T8445 |
631-638 |
NNS |
denotes |
samples |
T8446 |
600-603 |
CC |
denotes |
and |
T8447 |
604-609 |
NN |
denotes |
knock |
T8448 |
610-613 |
NN |
denotes |
out |
T8449 |
614-620 |
JJ |
denotes |
murine |
T8450 |
621-624 |
CC |
denotes |
and |
T8451 |
625-630 |
JJ |
denotes |
human |
T8452 |
638-639 |
. |
denotes |
. |
T8453 |
639-966 |
sentence |
denotes |
250 μl adjusted platelets were prewarmed to 37°C and used in the assay, and the aggregation process was started by adding 25 μl of a prewarmed 16.5 mg/ml ristocetin solution (DiaMed Diagnostica, Bensheim, FRG; concentration in the assay: 1.5 mg/ml), a reagent which worked reliably in our hands with mouse and human platelets. |
T8454 |
640-643 |
CD |
denotes |
250 |
T8455 |
644-646 |
NN |
denotes |
μl |
T8456 |
656-665 |
NNS |
denotes |
platelets |
T8457 |
647-655 |
VBN |
denotes |
adjusted |
T8458 |
671-680 |
VBN |
denotes |
prewarmed |
T8459 |
666-670 |
VBD |
denotes |
were |
T8460 |
681-683 |
IN |
denotes |
to |
T8461 |
684-686 |
CD |
denotes |
37 |
T8462 |
686-688 |
NNS |
denotes |
°C |
T8463 |
689-692 |
CC |
denotes |
and |
T8464 |
693-697 |
VBN |
denotes |
used |
T8465 |
698-700 |
IN |
denotes |
in |
T8466 |
701-704 |
DT |
denotes |
the |
T8467 |
705-710 |
NN |
denotes |
assay |
T8468 |
710-712 |
, |
denotes |
, |
T8469 |
712-715 |
CC |
denotes |
and |
T8470 |
716-719 |
DT |
denotes |
the |
T8471 |
732-739 |
NN |
denotes |
process |
T8472 |
720-731 |
NN |
denotes |
aggregation |
T8473 |
744-751 |
VBN |
denotes |
started |
T8474 |
740-743 |
VBD |
denotes |
was |
T8475 |
752-754 |
IN |
denotes |
by |
T8476 |
755-761 |
VBG |
denotes |
adding |
T8477 |
762-764 |
CD |
denotes |
25 |
T8478 |
765-767 |
NN |
denotes |
μl |
T8479 |
768-770 |
IN |
denotes |
of |
T8480 |
771-772 |
DT |
denotes |
a |
T8481 |
805-813 |
NN |
denotes |
solution |
T8482 |
773-782 |
JJ |
denotes |
prewarmed |
T8483 |
783-787 |
CD |
denotes |
16.5 |
T8484 |
788-790 |
NN |
denotes |
mg |
T8485 |
790-791 |
SYM |
denotes |
/ |
T8486 |
791-793 |
NN |
denotes |
ml |
T8487 |
794-804 |
NN |
denotes |
ristocetin |
T8488 |
814-815 |
-LRB- |
denotes |
( |
T8489 |
845-848 |
NNP |
denotes |
FRG |
T8490 |
815-821 |
NNP |
denotes |
DiaMed |
T8491 |
822-833 |
NNP |
denotes |
Diagnostica |
T8492 |
833-835 |
, |
denotes |
, |
T8493 |
835-843 |
NNP |
denotes |
Bensheim |
T8494 |
843-845 |
, |
denotes |
, |
T8495 |
848-849 |
: |
denotes |
; |
T8496 |
850-863 |
NN |
denotes |
concentration |
T8497 |
882-884 |
NNS |
denotes |
mg |
T8498 |
864-866 |
IN |
denotes |
in |
T8499 |
867-870 |
DT |
denotes |
the |
T8500 |
871-876 |
NN |
denotes |
assay |
T8501 |
876-878 |
: |
denotes |
: |
T8502 |
878-881 |
CD |
denotes |
1.5 |
T8503 |
884-885 |
SYM |
denotes |
/ |
T8504 |
885-887 |
NN |
denotes |
ml |
T8505 |
887-888 |
-RRB- |
denotes |
) |
T8506 |
888-890 |
, |
denotes |
, |
T8507 |
890-891 |
DT |
denotes |
a |
T8508 |
892-899 |
NN |
denotes |
reagent |
T8509 |
900-905 |
WDT |
denotes |
which |
T8510 |
906-912 |
VBD |
denotes |
worked |
T8511 |
913-921 |
RB |
denotes |
reliably |
T8512 |
922-924 |
IN |
denotes |
in |
T8513 |
925-928 |
PRP$ |
denotes |
our |
T8514 |
929-934 |
NNS |
denotes |
hands |
T8515 |
935-939 |
IN |
denotes |
with |
T8516 |
940-945 |
NN |
denotes |
mouse |
T8517 |
956-965 |
NNS |
denotes |
platelets |
T8518 |
946-949 |
CC |
denotes |
and |
T8519 |
950-955 |
JJ |
denotes |
human |
T8520 |
965-966 |
. |
denotes |
. |
T8521 |
966-1015 |
sentence |
denotes |
Each murine assay represents a different animal. |
T8522 |
967-971 |
DT |
denotes |
Each |
T8523 |
979-984 |
NN |
denotes |
assay |
T8524 |
972-978 |
JJ |
denotes |
murine |
T8525 |
985-995 |
VBZ |
denotes |
represents |
T8526 |
996-997 |
DT |
denotes |
a |
T8527 |
1008-1014 |
NN |
denotes |
animal |
T8528 |
998-1007 |
JJ |
denotes |
different |
T8529 |
1014-1015 |
. |
denotes |
. |
T8530 |
1015-1107 |
sentence |
denotes |
Analyses of human blood samples were performed as described in the manufacturer's protocol. |
T8531 |
1016-1024 |
NNS |
denotes |
Analyses |
T8532 |
1053-1062 |
VBN |
denotes |
performed |
T8533 |
1025-1027 |
IN |
denotes |
of |
T8534 |
1028-1033 |
JJ |
denotes |
human |
T8535 |
1040-1047 |
NNS |
denotes |
samples |
T8536 |
1034-1039 |
NN |
denotes |
blood |
T8537 |
1048-1052 |
VBD |
denotes |
were |
T8538 |
1063-1065 |
IN |
denotes |
as |
T8539 |
1066-1075 |
VBN |
denotes |
described |
T8540 |
1076-1078 |
IN |
denotes |
in |
T8541 |
1079-1082 |
DT |
denotes |
the |
T8542 |
1083-1095 |
NN |
denotes |
manufacturer |
T8543 |
1098-1106 |
NN |
denotes |
protocol |
T8544 |
1095-1097 |
POS |
denotes |
's |
T8545 |
1106-1107 |
. |
denotes |
. |
T8546 |
1107-1200 |
sentence |
denotes |
Several repeated measurements were done per human individual to control the reproducibility. |
T8547 |
1108-1115 |
JJ |
denotes |
Several |
T8548 |
1125-1137 |
NNS |
denotes |
measurements |
T8549 |
1116-1124 |
JJ |
denotes |
repeated |
T8550 |
1143-1147 |
VBN |
denotes |
done |
T8551 |
1138-1142 |
VBD |
denotes |
were |
T8552 |
1148-1151 |
IN |
denotes |
per |
T8553 |
1152-1157 |
JJ |
denotes |
human |
T8554 |
1158-1168 |
NN |
denotes |
individual |
T8555 |
1169-1171 |
TO |
denotes |
to |
T8556 |
1172-1179 |
VB |
denotes |
control |
T8557 |
1180-1183 |
DT |
denotes |
the |
T8558 |
1184-1199 |
NN |
denotes |
reproducibility |
T8559 |
1199-1200 |
. |
denotes |
. |
T8560 |
1200-1403 |
sentence |
denotes |
The acquired aggregation curve data, measured as ascending degree of transmission, were analysed for maximal slope values, while the maximal aggregation amplitude of every single curve was set to 100 %. |
T8561 |
1201-1204 |
DT |
denotes |
The |
T8562 |
1232-1236 |
NNS |
denotes |
data |
T8563 |
1205-1213 |
VBN |
denotes |
acquired |
T8564 |
1214-1225 |
NN |
denotes |
aggregation |
T8565 |
1226-1231 |
NN |
denotes |
curve |
T8566 |
1289-1297 |
VBN |
denotes |
analysed |
T8567 |
1236-1238 |
, |
denotes |
, |
T8568 |
1238-1246 |
VBN |
denotes |
measured |
T8569 |
1247-1249 |
IN |
denotes |
as |
T8570 |
1250-1259 |
VBG |
denotes |
ascending |
T8571 |
1260-1266 |
NN |
denotes |
degree |
T8572 |
1267-1269 |
IN |
denotes |
of |
T8573 |
1270-1282 |
NN |
denotes |
transmission |
T8574 |
1282-1284 |
, |
denotes |
, |
T8575 |
1284-1288 |
VBD |
denotes |
were |
T8576 |
1298-1301 |
IN |
denotes |
for |
T8577 |
1302-1309 |
JJ |
denotes |
maximal |
T8578 |
1316-1322 |
NNS |
denotes |
values |
T8579 |
1310-1315 |
NN |
denotes |
slope |
T8580 |
1322-1324 |
, |
denotes |
, |
T8581 |
1324-1329 |
IN |
denotes |
while |
T8582 |
1390-1393 |
VBN |
denotes |
set |
T8583 |
1330-1333 |
DT |
denotes |
the |
T8584 |
1354-1363 |
NN |
denotes |
amplitude |
T8585 |
1334-1341 |
JJ |
denotes |
maximal |
T8586 |
1342-1353 |
NN |
denotes |
aggregation |
T8587 |
1364-1366 |
IN |
denotes |
of |
T8588 |
1367-1372 |
DT |
denotes |
every |
T8589 |
1380-1385 |
NN |
denotes |
curve |
T8590 |
1373-1379 |
JJ |
denotes |
single |
T8591 |
1386-1389 |
VBD |
denotes |
was |
T8592 |
1394-1396 |
IN |
denotes |
to |
T8593 |
1397-1400 |
CD |
denotes |
100 |
T8594 |
1401-1402 |
NN |
denotes |
% |
T8595 |
1402-1403 |
. |
denotes |
. |
T8596 |
1403-1510 |
sentence |
denotes |
In general, all platelet procedures were done at 22°C within four hours after collecting the blood sample. |
T8597 |
1404-1406 |
IN |
denotes |
In |
T8598 |
1445-1449 |
VBN |
denotes |
done |
T8599 |
1407-1414 |
JJ |
denotes |
general |
T8600 |
1414-1416 |
, |
denotes |
, |
T8601 |
1416-1419 |
DT |
denotes |
all |
T8602 |
1429-1439 |
NNS |
denotes |
procedures |
T8603 |
1420-1428 |
NN |
denotes |
platelet |
T8604 |
1440-1444 |
VBD |
denotes |
were |
T8605 |
1450-1452 |
IN |
denotes |
at |
T8606 |
1453-1455 |
CD |
denotes |
22 |
T8607 |
1455-1457 |
NNS |
denotes |
°C |
T8608 |
1458-1464 |
IN |
denotes |
within |
T8609 |
1465-1469 |
CD |
denotes |
four |
T8610 |
1470-1475 |
NNS |
denotes |
hours |
T8611 |
1476-1481 |
IN |
denotes |
after |
T8612 |
1482-1492 |
VBG |
denotes |
collecting |
T8613 |
1493-1496 |
DT |
denotes |
the |
T8614 |
1503-1509 |
NN |
denotes |
sample |
T8615 |
1497-1502 |
NN |
denotes |
blood |
T8616 |
1509-1510 |
. |
denotes |
. |
R5167 |
T8326 |
T8327 |
compound |
Platelet,aggregation |
R5168 |
T8327 |
T8328 |
compound |
aggregation,assay |
R5169 |
T8330 |
T8331 |
advmod |
Approximately,500 |
R5170 |
T8331 |
T8332 |
nummod |
500,μl |
R5171 |
T8332 |
T8333 |
nsubjpass |
μl,centrifuged |
R5172 |
T8334 |
T8332 |
prep |
of,μl |
R5173 |
T8335 |
T8336 |
amod |
murine,blood |
R5174 |
T8336 |
T8334 |
pobj |
blood,of |
R5175 |
T8337 |
T8333 |
auxpass |
were,centrifuged |
R5176 |
T8338 |
T8333 |
prep |
at,centrifuged |
R5177 |
T8339 |
T8340 |
nummod |
200,g |
R5178 |
T8340 |
T8338 |
pobj |
g,at |
R5179 |
T8341 |
T8340 |
punct |
×,g |
R5180 |
T8342 |
T8333 |
prep |
for,centrifuged |
R5181 |
T8343 |
T8344 |
nummod |
15,minutes |
R5182 |
T8344 |
T8342 |
pobj |
minutes,for |
R5183 |
T8345 |
T8333 |
punct |
.,centrifuged |
R5184 |
T8347 |
T8348 |
det |
The,supernatants |
R5185 |
T8348 |
T8350 |
nsubjpass |
supernatants,transferred |
R5186 |
T8349 |
T8348 |
amod |
resulting,supernatants |
R5187 |
T8351 |
T8350 |
auxpass |
were,transferred |
R5188 |
T8352 |
T8350 |
prep |
to,transferred |
R5189 |
T8353 |
T8354 |
amod |
new,tubes |
R5190 |
T8354 |
T8352 |
pobj |
tubes,to |
R5191 |
T8355 |
T8350 |
cc |
and,transferred |
R5192 |
T8356 |
T8350 |
conj |
used,transferred |
R5193 |
T8357 |
T8356 |
prep |
as,used |
R5194 |
T8358 |
T8359 |
npadvmod |
platelet,rich |
R5195 |
T8359 |
T8360 |
amod |
rich,plasma |
R5196 |
T8360 |
T8357 |
pobj |
plasma,as |
R5197 |
T8361 |
T8360 |
punct |
(,plasma |
R5198 |
T8362 |
T8360 |
appos |
PRP,plasma |
R5199 |
T8363 |
T8350 |
punct |
),transferred |
R5200 |
T8364 |
T8350 |
punct |
.,transferred |
R5201 |
T8366 |
T8367 |
det |
The,measurements |
R5202 |
T8367 |
T8370 |
nsubjpass |
measurements,done |
R5203 |
T8368 |
T8369 |
compound |
platelet,aggregation |
R5204 |
T8369 |
T8367 |
compound |
aggregation,measurements |
R5205 |
T8371 |
T8370 |
auxpass |
were,done |
R5206 |
T8372 |
T8370 |
prep |
according,done |
R5207 |
T8373 |
T8372 |
prep |
to,according |
R5208 |
T8374 |
T8375 |
det |
the,method |
R5209 |
T8375 |
T8373 |
pobj |
method,to |
R5210 |
T8376 |
T8375 |
acl |
developed,method |
R5211 |
T8377 |
T8376 |
agent |
by,developed |
R5212 |
T8378 |
T8377 |
pobj |
Born,by |
R5213 |
T8379 |
T8378 |
cc |
and,Born |
R5214 |
T8380 |
T8378 |
conj |
Cross,Born |
R5215 |
T8381 |
T8382 |
punct |
[,26 |
R5216 |
T8382 |
T8380 |
parataxis |
26,Cross |
R5217 |
T8383 |
T8382 |
punct |
],26 |
R5218 |
T8384 |
T8370 |
advcl |
using,done |
R5219 |
T8385 |
T8386 |
det |
an,photometer |
R5220 |
T8386 |
T8384 |
dobj |
photometer,using |
R5221 |
T8387 |
T8386 |
compound |
APACT,photometer |
R5222 |
T8388 |
T8389 |
punct |
(,FRG |
R5223 |
T8389 |
T8386 |
parataxis |
FRG,photometer |
R5224 |
T8390 |
T8389 |
dep |
Labor,FRG |
R5225 |
T8391 |
T8389 |
punct |
", ",FRG |
R5226 |
T8392 |
T8389 |
dep |
Ahrendsburg,FRG |
R5227 |
T8393 |
T8389 |
punct |
", ",FRG |
R5228 |
T8394 |
T8389 |
punct |
),FRG |
R5229 |
T8395 |
T8386 |
cc |
and,photometer |
R5230 |
T8396 |
T8397 |
det |
the,software |
R5231 |
T8397 |
T8386 |
conj |
software,photometer |
R5232 |
T8398 |
T8397 |
compound |
APACT,software |
R5233 |
T8399 |
T8400 |
punct |
(,APACT |
R5234 |
T8400 |
T8397 |
parataxis |
APACT,software |
R5235 |
T8401 |
T8400 |
nummod |
1.4,APACT |
R5236 |
T8402 |
T8400 |
punct |
),APACT |
R5237 |
T8403 |
T8370 |
punct |
.,done |
R5238 |
T8405 |
T8406 |
advmod |
Subsequently,adjusted |
R5239 |
T8407 |
T8406 |
nsubjpass |
samples,adjusted |
R5240 |
T8408 |
T8407 |
prep |
of,samples |
R5241 |
T8409 |
T8410 |
det |
the,PRP |
R5242 |
T8410 |
T8408 |
pobj |
PRP,of |
R5243 |
T8411 |
T8406 |
auxpass |
were,adjusted |
R5244 |
T8412 |
T8406 |
prep |
with,adjusted |
R5245 |
T8413 |
T8412 |
pobj |
buffer,with |
R5246 |
T8414 |
T8415 |
punct |
(,pH |
R5247 |
T8415 |
T8413 |
parataxis |
pH,buffer |
R5248 |
T8416 |
T8417 |
nummod |
5,mM |
R5249 |
T8417 |
T8418 |
compound |
mM,Hepes |
R5250 |
T8418 |
T8415 |
dep |
Hepes,pH |
R5251 |
T8419 |
T8415 |
punct |
", ",pH |
R5252 |
T8420 |
T8421 |
nummod |
150,mM |
R5253 |
T8421 |
T8422 |
compound |
mM,NaCl |
R5254 |
T8422 |
T8415 |
dep |
NaCl,pH |
R5255 |
T8423 |
T8415 |
punct |
", ",pH |
R5256 |
T8424 |
T8415 |
nummod |
7.3,pH |
R5257 |
T8425 |
T8415 |
punct |
),pH |
R5258 |
T8426 |
T8406 |
prep |
to,adjusted |
R5259 |
T8427 |
T8428 |
nummod |
"250,000",platelets |
R5260 |
T8428 |
T8426 |
pobj |
platelets,to |
R5261 |
T8429 |
T8430 |
punct |
/,μl |
R5262 |
T8430 |
T8428 |
prep |
μl,platelets |
R5263 |
T8431 |
T8406 |
punct |
.,adjusted |
R5264 |
T8433 |
T8434 |
det |
A,count |
R5265 |
T8434 |
T8437 |
nsubjpass |
count,used |
R5266 |
T8435 |
T8434 |
amod |
constant,count |
R5267 |
T8436 |
T8434 |
compound |
platelet,count |
R5268 |
T8438 |
T8437 |
auxpass |
was,used |
R5269 |
T8439 |
T8437 |
advmod |
throughout,used |
R5270 |
T8440 |
T8437 |
advcl |
allowing,used |
R5271 |
T8441 |
T8440 |
dobj |
comparisons,allowing |
R5272 |
T8442 |
T8441 |
prep |
between,comparisons |
R5273 |
T8443 |
T8444 |
amod |
wild,type |
R5274 |
T8444 |
T8445 |
nmod |
type,samples |
R5275 |
T8445 |
T8442 |
pobj |
samples,between |
R5276 |
T8446 |
T8444 |
cc |
and,type |
R5277 |
T8447 |
T8448 |
compound |
knock,out |
R5278 |
T8448 |
T8444 |
conj |
out,type |
R5279 |
T8449 |
T8445 |
amod |
murine,samples |
R5280 |
T8450 |
T8449 |
cc |
and,murine |
R5281 |
T8451 |
T8449 |
conj |
human,murine |
R5282 |
T8452 |
T8437 |
punct |
.,used |
R5283 |
T8454 |
T8455 |
nummod |
250,μl |
R5284 |
T8455 |
T8456 |
nmod |
μl,platelets |
R5285 |
T8456 |
T8458 |
nsubjpass |
platelets,prewarmed |
R5286 |
T8457 |
T8456 |
amod |
adjusted,platelets |
R5287 |
T8459 |
T8458 |
auxpass |
were,prewarmed |
R5288 |
T8460 |
T8458 |
prep |
to,prewarmed |
R5289 |
T8461 |
T8462 |
nummod |
37,°C |
R5290 |
T8462 |
T8460 |
pobj |
°C,to |
R5291 |
T8463 |
T8458 |
cc |
and,prewarmed |
R5292 |
T8464 |
T8458 |
conj |
used,prewarmed |
R5293 |
T8465 |
T8464 |
prep |
in,used |
R5294 |
T8466 |
T8467 |
det |
the,assay |
R5295 |
T8467 |
T8465 |
pobj |
assay,in |
R5296 |
T8468 |
T8458 |
punct |
", ",prewarmed |
R5297 |
T8469 |
T8458 |
cc |
and,prewarmed |
R5298 |
T8470 |
T8471 |
det |
the,process |
R5299 |
T8471 |
T8473 |
nsubjpass |
process,started |
R5300 |
T8472 |
T8471 |
compound |
aggregation,process |
R5301 |
T8473 |
T8458 |
conj |
started,prewarmed |
R5302 |
T8474 |
T8473 |
auxpass |
was,started |
R5303 |
T8475 |
T8473 |
prep |
by,started |
R5304 |
T8476 |
T8475 |
pcomp |
adding,by |
R5305 |
T8477 |
T8478 |
nummod |
25,μl |
R5306 |
T8478 |
T8476 |
dobj |
μl,adding |
R5307 |
T8479 |
T8478 |
prep |
of,μl |
R5308 |
T8480 |
T8481 |
det |
a,solution |
R5309 |
T8481 |
T8479 |
pobj |
solution,of |
R5310 |
T8482 |
T8481 |
amod |
prewarmed,solution |
R5311 |
T8483 |
T8484 |
nummod |
16.5,mg |
R5312 |
T8484 |
T8481 |
nmod |
mg,solution |
R5313 |
T8485 |
T8486 |
punct |
/,ml |
R5314 |
T8486 |
T8484 |
prep |
ml,mg |
R5315 |
T8487 |
T8481 |
compound |
ristocetin,solution |
R5316 |
T8488 |
T8489 |
punct |
(,FRG |
R5317 |
T8489 |
T8481 |
parataxis |
FRG,solution |
R5318 |
T8490 |
T8491 |
compound |
DiaMed,Diagnostica |
R5319 |
T8491 |
T8489 |
dep |
Diagnostica,FRG |
R5320 |
T8492 |
T8489 |
punct |
", ",FRG |
R5321 |
T8493 |
T8489 |
dep |
Bensheim,FRG |
R5322 |
T8494 |
T8489 |
punct |
", ",FRG |
R5323 |
T8495 |
T8489 |
punct |
;,FRG |
R5324 |
T8496 |
T8497 |
dep |
concentration,mg |
R5325 |
T8497 |
T8489 |
dep |
mg,FRG |
R5326 |
T8498 |
T8496 |
prep |
in,concentration |
R5327 |
T8499 |
T8500 |
det |
the,assay |
R5328 |
T8500 |
T8498 |
pobj |
assay,in |
R5329 |
T8501 |
T8497 |
punct |
: ,mg |
R5330 |
T8502 |
T8497 |
nummod |
1.5,mg |
R5331 |
T8503 |
T8504 |
punct |
/,ml |
R5332 |
T8504 |
T8497 |
prep |
ml,mg |
R5333 |
T8505 |
T8489 |
punct |
),FRG |
R5334 |
T8506 |
T8481 |
punct |
", ",solution |
R5335 |
T8507 |
T8508 |
det |
a,reagent |
R5336 |
T8508 |
T8481 |
appos |
reagent,solution |
R5337 |
T8509 |
T8510 |
dep |
which,worked |
R5338 |
T8510 |
T8508 |
relcl |
worked,reagent |
R5339 |
T8511 |
T8510 |
advmod |
reliably,worked |
R5340 |
T8512 |
T8510 |
prep |
in,worked |
R5341 |
T8513 |
T8514 |
poss |
our,hands |
R5342 |
T8514 |
T8512 |
pobj |
hands,in |
R5343 |
T8515 |
T8510 |
prep |
with,worked |
R5344 |
T8516 |
T8517 |
nmod |
mouse,platelets |
R5345 |
T8517 |
T8515 |
pobj |
platelets,with |
R5346 |
T8518 |
T8516 |
cc |
and,mouse |
R5347 |
T8519 |
T8516 |
conj |
human,mouse |
R5348 |
T8520 |
T8473 |
punct |
.,started |
R5349 |
T8522 |
T8523 |
det |
Each,assay |
R5350 |
T8523 |
T8525 |
nsubj |
assay,represents |
R5351 |
T8524 |
T8523 |
amod |
murine,assay |
R5352 |
T8526 |
T8527 |
det |
a,animal |
R5353 |
T8527 |
T8525 |
dobj |
animal,represents |
R5354 |
T8528 |
T8527 |
amod |
different,animal |
R5355 |
T8529 |
T8525 |
punct |
.,represents |
R5356 |
T8531 |
T8532 |
nsubjpass |
Analyses,performed |
R5357 |
T8533 |
T8531 |
prep |
of,Analyses |
R5358 |
T8534 |
T8535 |
amod |
human,samples |
R5359 |
T8535 |
T8533 |
pobj |
samples,of |
R5360 |
T8536 |
T8535 |
compound |
blood,samples |
R5361 |
T8537 |
T8532 |
auxpass |
were,performed |
R5362 |
T8538 |
T8539 |
mark |
as,described |
R5363 |
T8539 |
T8532 |
advcl |
described,performed |
R5364 |
T8540 |
T8539 |
prep |
in,described |
R5365 |
T8541 |
T8542 |
det |
the,manufacturer |
R5366 |
T8542 |
T8543 |
poss |
manufacturer,protocol |
R5367 |
T8543 |
T8540 |
pobj |
protocol,in |
R5368 |
T8544 |
T8542 |
case |
's,manufacturer |
R5369 |
T8545 |
T8532 |
punct |
.,performed |
R5370 |
T8547 |
T8548 |
amod |
Several,measurements |
R5371 |
T8548 |
T8550 |
nsubjpass |
measurements,done |
R5372 |
T8549 |
T8548 |
amod |
repeated,measurements |
R5373 |
T8551 |
T8550 |
auxpass |
were,done |
R5374 |
T8552 |
T8550 |
prep |
per,done |
R5375 |
T8553 |
T8554 |
amod |
human,individual |
R5376 |
T8554 |
T8552 |
pobj |
individual,per |
R5377 |
T8555 |
T8556 |
aux |
to,control |
R5378 |
T8556 |
T8550 |
advcl |
control,done |
R5379 |
T8557 |
T8558 |
det |
the,reproducibility |
R5380 |
T8558 |
T8556 |
dobj |
reproducibility,control |
R5381 |
T8559 |
T8550 |
punct |
.,done |
R5382 |
T8561 |
T8562 |
det |
The,data |
R5383 |
T8562 |
T8566 |
nsubjpass |
data,analysed |
R5384 |
T8563 |
T8562 |
amod |
acquired,data |
R5385 |
T8564 |
T8565 |
compound |
aggregation,curve |
R5386 |
T8565 |
T8562 |
compound |
curve,data |
R5387 |
T8567 |
T8562 |
punct |
", ",data |
R5388 |
T8568 |
T8562 |
acl |
measured,data |
R5389 |
T8569 |
T8568 |
prep |
as,measured |
R5390 |
T8570 |
T8571 |
amod |
ascending,degree |
R5391 |
T8571 |
T8569 |
pobj |
degree,as |
R5392 |
T8572 |
T8571 |
prep |
of,degree |
R5393 |
T8573 |
T8572 |
pobj |
transmission,of |
R5394 |
T8574 |
T8566 |
punct |
", ",analysed |
R5395 |
T8575 |
T8566 |
auxpass |
were,analysed |
R5396 |
T8576 |
T8566 |
prep |
for,analysed |
R5397 |
T8577 |
T8578 |
amod |
maximal,values |
R5398 |
T8578 |
T8576 |
pobj |
values,for |
R5399 |
T8579 |
T8578 |
compound |
slope,values |
R5400 |
T8580 |
T8566 |
punct |
", ",analysed |
R5401 |
T8581 |
T8582 |
prep |
while,set |
R5402 |
T8582 |
T8566 |
advcl |
set,analysed |
R5403 |
T8583 |
T8584 |
det |
the,amplitude |
R5404 |
T8584 |
T8582 |
nsubjpass |
amplitude,set |
R5405 |
T8585 |
T8584 |
amod |
maximal,amplitude |
R5406 |
T8586 |
T8584 |
compound |
aggregation,amplitude |
R5407 |
T8587 |
T8584 |
prep |
of,amplitude |
R5408 |
T8588 |
T8589 |
det |
every,curve |
R5409 |
T8589 |
T8587 |
pobj |
curve,of |
R5410 |
T8590 |
T8589 |
amod |
single,curve |
R5411 |
T8591 |
T8582 |
auxpass |
was,set |
R5412 |
T8592 |
T8582 |
prep |
to,set |
R5413 |
T8593 |
T8594 |
nummod |
100,% |
R5414 |
T8594 |
T8592 |
pobj |
%,to |
R5415 |
T8595 |
T8566 |
punct |
.,analysed |
R5416 |
T8597 |
T8598 |
prep |
In,done |
R5417 |
T8599 |
T8597 |
amod |
general,In |
R5418 |
T8600 |
T8598 |
punct |
", ",done |
R5419 |
T8601 |
T8602 |
det |
all,procedures |
R5420 |
T8602 |
T8598 |
nsubjpass |
procedures,done |
R5421 |
T8603 |
T8602 |
compound |
platelet,procedures |
R5422 |
T8604 |
T8598 |
auxpass |
were,done |
R5423 |
T8605 |
T8598 |
prep |
at,done |
R5424 |
T8606 |
T8607 |
nummod |
22,°C |
R5425 |
T8607 |
T8605 |
pobj |
°C,at |
R5426 |
T8608 |
T8598 |
prep |
within,done |
R5427 |
T8609 |
T8610 |
nummod |
four,hours |
R5428 |
T8610 |
T8608 |
pobj |
hours,within |
R5429 |
T8611 |
T8610 |
prep |
after,hours |
R5430 |
T8612 |
T8611 |
pcomp |
collecting,after |
R5431 |
T8613 |
T8614 |
det |
the,sample |
R5432 |
T8614 |
T8612 |
dobj |
sample,collecting |
R5433 |
T8615 |
T8614 |
compound |
blood,sample |
R5434 |
T8616 |
T8598 |
punct |
.,done |