Id |
Subject |
Object |
Predicate |
Lexical cue |
T7951 |
0-13 |
NN |
denotes |
Acetylcholine |
T7952 |
14-22 |
NN |
denotes |
esterase |
T7953 |
23-28 |
NN |
denotes |
assay |
T7954 |
28-213 |
sentence |
denotes |
A 30 μl sample of red blood cell vesicles was mixed with an equal volume of buffer (20 mM Hepes, pH 7.6, 150 mM NaCl, 0.5 % Triton X-100), vortexed and incubated for 5 minutes at 37°C. |
T7955 |
29-30 |
DT |
denotes |
A |
T7956 |
37-43 |
NN |
denotes |
sample |
T7957 |
31-33 |
CD |
denotes |
30 |
T7958 |
34-36 |
NN |
denotes |
μl |
T7959 |
75-80 |
VBN |
denotes |
mixed |
T7960 |
44-46 |
IN |
denotes |
of |
T7961 |
47-50 |
JJ |
denotes |
red |
T7962 |
57-61 |
NN |
denotes |
cell |
T7963 |
51-56 |
NN |
denotes |
blood |
T7964 |
62-70 |
NNS |
denotes |
vesicles |
T7965 |
71-74 |
VBD |
denotes |
was |
T7966 |
81-85 |
IN |
denotes |
with |
T7967 |
86-88 |
DT |
denotes |
an |
T7968 |
95-101 |
NN |
denotes |
volume |
T7969 |
89-94 |
JJ |
denotes |
equal |
T7970 |
102-104 |
IN |
denotes |
of |
T7971 |
105-111 |
NN |
denotes |
buffer |
T7972 |
112-113 |
-LRB- |
denotes |
( |
T7973 |
160-161 |
NN |
denotes |
X |
T7974 |
113-115 |
CD |
denotes |
20 |
T7975 |
116-118 |
NN |
denotes |
mM |
T7976 |
119-124 |
NN |
denotes |
Hepes |
T7977 |
124-126 |
, |
denotes |
, |
T7978 |
126-128 |
NN |
denotes |
pH |
T7979 |
129-132 |
CD |
denotes |
7.6 |
T7980 |
132-134 |
, |
denotes |
, |
T7981 |
134-137 |
CD |
denotes |
150 |
T7982 |
138-140 |
NN |
denotes |
mM |
T7983 |
141-145 |
NN |
denotes |
NaCl |
T7984 |
145-147 |
, |
denotes |
, |
T7985 |
147-150 |
CD |
denotes |
0.5 |
T7986 |
151-152 |
NN |
denotes |
% |
T7987 |
153-159 |
NN |
denotes |
Triton |
T7988 |
161-162 |
HYPH |
denotes |
- |
T7989 |
162-165 |
CD |
denotes |
100 |
T7990 |
165-166 |
-RRB- |
denotes |
) |
T7991 |
166-168 |
, |
denotes |
, |
T7992 |
168-176 |
VBN |
denotes |
vortexed |
T7993 |
177-180 |
CC |
denotes |
and |
T7994 |
181-190 |
VBN |
denotes |
incubated |
T7995 |
191-194 |
IN |
denotes |
for |
T7996 |
195-196 |
CD |
denotes |
5 |
T7997 |
197-204 |
NNS |
denotes |
minutes |
T7998 |
205-207 |
IN |
denotes |
at |
T7999 |
208-210 |
CD |
denotes |
37 |
T8000 |
210-212 |
NNS |
denotes |
°C |
T8001 |
212-213 |
. |
denotes |
. |
T8002 |
213-433 |
sentence |
denotes |
Subsequently 640 μl sodium phosphate buffer (100 mM Na2PO4, pH 7.6), 50 μl DTNB (5,5'-dithiobis-(2-nitrobenzoic acid), 10 mM in sodium phosphate buffer), and 50 μl acetylthiocholine chloride (12,5 mM in H2O) were added. |
T8003 |
214-226 |
RB |
denotes |
Subsequently |
T8004 |
427-432 |
VBN |
denotes |
added |
T8005 |
227-230 |
CD |
denotes |
640 |
T8006 |
231-233 |
NN |
denotes |
μl |
T8007 |
251-257 |
NN |
denotes |
buffer |
T8008 |
234-240 |
NN |
denotes |
sodium |
T8009 |
241-250 |
NN |
denotes |
phosphate |
T8010 |
258-259 |
-LRB- |
denotes |
( |
T8011 |
266-272 |
NN |
denotes |
Na2PO4 |
T8012 |
259-262 |
CD |
denotes |
100 |
T8013 |
263-265 |
NN |
denotes |
mM |
T8014 |
272-274 |
, |
denotes |
, |
T8015 |
274-276 |
NN |
denotes |
pH |
T8016 |
277-280 |
CD |
denotes |
7.6 |
T8017 |
280-281 |
-RRB- |
denotes |
) |
T8018 |
281-283 |
, |
denotes |
, |
T8019 |
283-285 |
CD |
denotes |
50 |
T8020 |
286-288 |
NN |
denotes |
μl |
T8021 |
289-293 |
NN |
denotes |
DTNB |
T8022 |
294-295 |
-LRB- |
denotes |
( |
T8023 |
336-338 |
NN |
denotes |
mM |
T8024 |
295-296 |
CD |
denotes |
5 |
T8025 |
300-309 |
NN |
denotes |
dithiobis |
T8026 |
296-297 |
, |
denotes |
, |
T8027 |
297-298 |
CD |
denotes |
5 |
T8028 |
298-299 |
SYM |
denotes |
' |
T8029 |
299-300 |
HYPH |
denotes |
- |
T8030 |
309-310 |
HYPH |
denotes |
- |
T8031 |
310-311 |
-LRB- |
denotes |
( |
T8032 |
326-330 |
NN |
denotes |
acid |
T8033 |
311-312 |
CD |
denotes |
2 |
T8034 |
312-313 |
HYPH |
denotes |
- |
T8035 |
313-325 |
JJ |
denotes |
nitrobenzoic |
T8036 |
330-331 |
-RRB- |
denotes |
) |
T8037 |
331-333 |
, |
denotes |
, |
T8038 |
333-335 |
CD |
denotes |
10 |
T8039 |
339-341 |
IN |
denotes |
in |
T8040 |
342-348 |
NN |
denotes |
sodium |
T8041 |
349-358 |
NN |
denotes |
phosphate |
T8042 |
359-365 |
NN |
denotes |
buffer |
T8043 |
365-366 |
-RRB- |
denotes |
) |
T8044 |
366-368 |
, |
denotes |
, |
T8045 |
368-371 |
CC |
denotes |
and |
T8046 |
372-374 |
CD |
denotes |
50 |
T8047 |
375-377 |
NN |
denotes |
μl |
T8048 |
396-404 |
NN |
denotes |
chloride |
T8049 |
378-395 |
NN |
denotes |
acetylthiocholine |
T8050 |
405-406 |
-LRB- |
denotes |
( |
T8051 |
411-413 |
NN |
denotes |
mM |
T8052 |
406-408 |
CD |
denotes |
12 |
T8053 |
408-409 |
, |
denotes |
, |
T8054 |
409-410 |
CD |
denotes |
5 |
T8055 |
414-416 |
IN |
denotes |
in |
T8056 |
417-420 |
NN |
denotes |
H2O |
T8057 |
420-421 |
-RRB- |
denotes |
) |
T8058 |
422-426 |
VBD |
denotes |
were |
T8059 |
432-433 |
. |
denotes |
. |
T8060 |
433-575 |
sentence |
denotes |
The reaction took place at room temperature and was measured photometrically at a wavelength of 405 nm according to the Ellmann' method [25]. |
T8061 |
434-437 |
DT |
denotes |
The |
T8062 |
438-446 |
NN |
denotes |
reaction |
T8063 |
447-451 |
VBD |
denotes |
took |
T8064 |
452-457 |
NN |
denotes |
place |
T8065 |
458-460 |
IN |
denotes |
at |
T8066 |
461-465 |
NN |
denotes |
room |
T8067 |
466-477 |
NN |
denotes |
temperature |
T8068 |
478-481 |
CC |
denotes |
and |
T8069 |
482-485 |
VBD |
denotes |
was |
T8070 |
486-494 |
VBN |
denotes |
measured |
T8071 |
495-510 |
RB |
denotes |
photometrically |
T8072 |
511-513 |
IN |
denotes |
at |
T8073 |
514-515 |
DT |
denotes |
a |
T8074 |
516-526 |
NN |
denotes |
wavelength |
T8075 |
527-529 |
IN |
denotes |
of |
T8076 |
530-533 |
CD |
denotes |
405 |
T8077 |
534-536 |
NNS |
denotes |
nm |
T8078 |
537-546 |
VBG |
denotes |
according |
T8079 |
547-549 |
IN |
denotes |
to |
T8080 |
550-553 |
DT |
denotes |
the |
T8081 |
563-569 |
NN |
denotes |
method |
T8082 |
554-561 |
NNP |
denotes |
Ellmann |
T8083 |
561-562 |
POS |
denotes |
' |
T8084 |
570-571 |
-LRB- |
denotes |
[ |
T8085 |
571-573 |
CD |
denotes |
25 |
T8086 |
573-574 |
-RRB- |
denotes |
] |
T8087 |
574-575 |
. |
denotes |
. |
R4913 |
T7951 |
T7952 |
compound |
Acetylcholine,esterase |
R4914 |
T7952 |
T7953 |
compound |
esterase,assay |
R4915 |
T7955 |
T7956 |
det |
A,sample |
R4916 |
T7956 |
T7959 |
nsubjpass |
sample,mixed |
R4917 |
T7957 |
T7958 |
nummod |
30,μl |
R4918 |
T7958 |
T7956 |
compound |
μl,sample |
R4919 |
T7960 |
T7956 |
prep |
of,sample |
R4920 |
T7961 |
T7962 |
amod |
red,cell |
R4921 |
T7962 |
T7964 |
compound |
cell,vesicles |
R4922 |
T7963 |
T7962 |
compound |
blood,cell |
R4923 |
T7964 |
T7960 |
pobj |
vesicles,of |
R4924 |
T7965 |
T7959 |
auxpass |
was,mixed |
R4925 |
T7966 |
T7959 |
prep |
with,mixed |
R4926 |
T7967 |
T7968 |
det |
an,volume |
R4927 |
T7968 |
T7966 |
pobj |
volume,with |
R4928 |
T7969 |
T7968 |
amod |
equal,volume |
R4929 |
T7970 |
T7968 |
prep |
of,volume |
R4930 |
T7971 |
T7970 |
pobj |
buffer,of |
R4931 |
T7972 |
T7973 |
punct |
(,X |
R4932 |
T7973 |
T7971 |
parataxis |
X,buffer |
R4933 |
T7974 |
T7975 |
nummod |
20,mM |
R4934 |
T7975 |
T7976 |
compound |
mM,Hepes |
R4935 |
T7976 |
T7973 |
dep |
Hepes,X |
R4936 |
T7977 |
T7976 |
punct |
", ",Hepes |
R4937 |
T7978 |
T7976 |
npadvmod |
pH,Hepes |
R4938 |
T7979 |
T7978 |
nummod |
7.6,pH |
R4939 |
T7980 |
T7973 |
punct |
", ",X |
R4940 |
T7981 |
T7982 |
nummod |
150,mM |
R4941 |
T7982 |
T7983 |
compound |
mM,NaCl |
R4942 |
T7983 |
T7973 |
dep |
NaCl,X |
R4943 |
T7984 |
T7973 |
punct |
", ",X |
R4944 |
T7985 |
T7986 |
nummod |
0.5,% |
R4945 |
T7986 |
T7973 |
compound |
%,X |
R4946 |
T7987 |
T7973 |
compound |
Triton,X |
R4947 |
T7988 |
T7973 |
punct |
-,X |
R4948 |
T7989 |
T7973 |
nummod |
100,X |
R4949 |
T7990 |
T7973 |
punct |
),X |
R4950 |
T7991 |
T7959 |
punct |
", ",mixed |
R4951 |
T7992 |
T7959 |
conj |
vortexed,mixed |
R4952 |
T7993 |
T7992 |
cc |
and,vortexed |
R4953 |
T7994 |
T7992 |
conj |
incubated,vortexed |
R4954 |
T7995 |
T7994 |
prep |
for,incubated |
R4955 |
T7996 |
T7997 |
nummod |
5,minutes |
R4956 |
T7997 |
T7995 |
pobj |
minutes,for |
R4957 |
T7998 |
T7994 |
prep |
at,incubated |
R4958 |
T7999 |
T8000 |
nummod |
37,°C |
R4959 |
T8000 |
T7998 |
pobj |
°C,at |
R4960 |
T8001 |
T7959 |
punct |
.,mixed |
R4961 |
T8003 |
T8004 |
advmod |
Subsequently,added |
R4962 |
T8005 |
T8006 |
nummod |
640,μl |
R4963 |
T8006 |
T8007 |
compound |
μl,buffer |
R4964 |
T8007 |
T8004 |
nsubjpass |
buffer,added |
R4965 |
T8008 |
T8009 |
compound |
sodium,phosphate |
R4966 |
T8009 |
T8007 |
compound |
phosphate,buffer |
R4967 |
T8010 |
T8011 |
punct |
(,Na2PO4 |
R4968 |
T8011 |
T8007 |
parataxis |
Na2PO4,buffer |
R4969 |
T8012 |
T8013 |
nummod |
100,mM |
R4970 |
T8013 |
T8011 |
compound |
mM,Na2PO4 |
R4971 |
T8014 |
T8011 |
punct |
", ",Na2PO4 |
R4972 |
T8015 |
T8011 |
npadvmod |
pH,Na2PO4 |
R4973 |
T8016 |
T8015 |
nummod |
7.6,pH |
R4974 |
T8017 |
T8011 |
punct |
),Na2PO4 |
R4975 |
T8018 |
T8007 |
punct |
", ",buffer |
R4976 |
T8019 |
T8020 |
nummod |
50,μl |
R4977 |
T8020 |
T8021 |
compound |
μl,DTNB |
R4978 |
T8021 |
T8007 |
conj |
DTNB,buffer |
R4979 |
T8022 |
T8023 |
punct |
(,mM |
R4980 |
T8023 |
T8021 |
parataxis |
mM,DTNB |
R4981 |
T8024 |
T8025 |
nummod |
5,dithiobis |
R4982 |
T8025 |
T8023 |
dep |
dithiobis,mM |
R4983 |
T8026 |
T8025 |
punct |
",",dithiobis |
R4984 |
T8027 |
T8025 |
nummod |
5,dithiobis |
R4985 |
T8028 |
T8027 |
punct |
',5 |
R4986 |
T8029 |
T8025 |
punct |
-,dithiobis |
R4987 |
T8030 |
T8023 |
punct |
-,mM |
R4988 |
T8031 |
T8032 |
punct |
(,acid |
R4989 |
T8032 |
T8023 |
parataxis |
acid,mM |
R4990 |
T8033 |
T8032 |
nummod |
2,acid |
R4991 |
T8034 |
T8032 |
punct |
-,acid |
R4992 |
T8035 |
T8032 |
amod |
nitrobenzoic,acid |
R4993 |
T8036 |
T8032 |
punct |
),acid |
R4994 |
T8037 |
T8023 |
punct |
", ",mM |
R4995 |
T8038 |
T8023 |
nummod |
10,mM |
R4996 |
T8039 |
T8023 |
prep |
in,mM |
R4997 |
T8040 |
T8041 |
compound |
sodium,phosphate |
R4998 |
T8041 |
T8042 |
compound |
phosphate,buffer |
R4999 |
T8042 |
T8039 |
pobj |
buffer,in |
R5000 |
T8043 |
T8023 |
punct |
),mM |
R5001 |
T8044 |
T8021 |
punct |
", ",DTNB |
R5002 |
T8045 |
T8021 |
cc |
and,DTNB |
R5003 |
T8046 |
T8047 |
nummod |
50,μl |
R5004 |
T8047 |
T8048 |
compound |
μl,chloride |
R5005 |
T8048 |
T8021 |
conj |
chloride,DTNB |
R5006 |
T8049 |
T8048 |
compound |
acetylthiocholine,chloride |
R5007 |
T8050 |
T8051 |
punct |
(,mM |
R5008 |
T8051 |
T8048 |
parataxis |
mM,chloride |
R5009 |
T8052 |
T8051 |
nummod |
12,mM |
R5010 |
T8053 |
T8051 |
punct |
",",mM |
R5011 |
T8054 |
T8051 |
nummod |
5,mM |
R5012 |
T8055 |
T8051 |
prep |
in,mM |
R5013 |
T8056 |
T8055 |
pobj |
H2O,in |
R5014 |
T8057 |
T8051 |
punct |
),mM |
R5015 |
T8058 |
T8004 |
auxpass |
were,added |
R5016 |
T8059 |
T8004 |
punct |
.,added |
R5017 |
T8061 |
T8062 |
det |
The,reaction |
R5018 |
T8062 |
T8063 |
nsubj |
reaction,took |
R5019 |
T8064 |
T8063 |
dobj |
place,took |
R5020 |
T8065 |
T8063 |
prep |
at,took |
R5021 |
T8066 |
T8067 |
compound |
room,temperature |
R5022 |
T8067 |
T8065 |
pobj |
temperature,at |
R5023 |
T8068 |
T8063 |
cc |
and,took |
R5024 |
T8069 |
T8070 |
auxpass |
was,measured |
R5025 |
T8070 |
T8063 |
conj |
measured,took |
R5026 |
T8071 |
T8070 |
advmod |
photometrically,measured |
R5027 |
T8072 |
T8070 |
prep |
at,measured |
R5028 |
T8073 |
T8074 |
det |
a,wavelength |
R5029 |
T8074 |
T8072 |
pobj |
wavelength,at |
R5030 |
T8075 |
T8074 |
prep |
of,wavelength |
R5031 |
T8076 |
T8077 |
nummod |
405,nm |
R5032 |
T8077 |
T8075 |
pobj |
nm,of |
R5033 |
T8078 |
T8070 |
prep |
according,measured |
R5034 |
T8079 |
T8078 |
prep |
to,according |
R5035 |
T8080 |
T8081 |
det |
the,method |
R5036 |
T8081 |
T8079 |
pobj |
method,to |
R5037 |
T8082 |
T8081 |
nmod |
Ellmann,method |
R5038 |
T8083 |
T8081 |
case |
',method |
R5039 |
T8084 |
T8085 |
punct |
[,25 |
R5040 |
T8085 |
T8070 |
parataxis |
25,measured |
R5041 |
T8086 |
T8085 |
punct |
],25 |
R5042 |
T8087 |
T8063 |
punct |
.,took |