PMC:194730 / 31244-31571
Annnotations
craft-sa-dev
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esterase assay\nA 30 μl sample of red blood cell vesicles was mixed with an equal volume of buffer (20 mM Hepes, pH 7.6, 150 mM NaCl, 0.5 % Triton X-100), vortexed and incubated for 5 minutes at 37°C. 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craft-ca-core-dev
{"project":"craft-ca-core-dev","denotations":[{"id":"T7922","span":{"begin":6,"end":19},"obj":"CHEBI:15355"},{"id":"T7923","span":{"begin":53,"end":67},"obj":"CL:0000232"},{"id":"T7924","span":{"begin":57,"end":62},"obj":"UBERON:0000178"},{"id":"T7925","span":{"begin":68,"end":76},"obj":"GO:0031982"},{"id":"T7926","span":{"begin":125,"end":130},"obj":"CHEBI:46756"},{"id":"T7927","span":{"begin":147,"end":151},"obj":"CHEBI:26710"},{"id":"T7928","span":{"begin":159,"end":171},"obj":"CHEBI:9750"},{"id":"T7929","span":{"begin":240,"end":256},"obj":"CHEBI:37586"},{"id":"T7930","span":{"begin":295,"end":299},"obj":"CHEBI:86228"}],"text":"sis.\n\nAcetylcholine esterase assay\nA 30 μl sample of red blood cell vesicles was mixed with an equal volume of buffer (20 mM Hepes, pH 7.6, 150 mM NaCl, 0.5 % Triton X-100), vortexed and incubated for 5 minutes at 37°C. Subsequently 640 μl sodium phosphate buffer (100 mM Na2PO4, pH 7.6), 50 μl DTNB (5,5'-dithiobis-(2-nitroben"}
craft-ca-core-ex-dev
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