Id |
Subject |
Object |
Predicate |
Lexical cue |
T7632 |
0-10 |
NN |
denotes |
Exovesicle |
T7633 |
11-21 |
NN |
denotes |
generation |
T7634 |
22-26 |
IN |
denotes |
from |
T7635 |
27-32 |
JJ |
denotes |
human |
T7636 |
54-59 |
NNS |
denotes |
cells |
T7637 |
33-36 |
CC |
denotes |
and |
T7638 |
37-43 |
JJ |
denotes |
murine |
T7639 |
44-47 |
JJ |
denotes |
red |
T7640 |
48-53 |
NN |
denotes |
blood |
T7641 |
59-129 |
sentence |
denotes |
Red blood cells naturally can form hemoglobin containing exovesicles. |
T7642 |
60-63 |
JJ |
denotes |
Red |
T7643 |
70-75 |
NNS |
denotes |
cells |
T7644 |
64-69 |
NN |
denotes |
blood |
T7645 |
90-94 |
VB |
denotes |
form |
T7646 |
76-85 |
RB |
denotes |
naturally |
T7647 |
86-89 |
MD |
denotes |
can |
T7648 |
95-105 |
NN |
denotes |
hemoglobin |
T7649 |
106-116 |
VBG |
denotes |
containing |
T7650 |
117-128 |
NNS |
denotes |
exovesicles |
T7651 |
128-129 |
. |
denotes |
. |
T7652 |
129-179 |
sentence |
denotes |
In vitro these vesicle formation is Ca2+ induced. |
T7653 |
130-132 |
FW |
denotes |
In |
T7654 |
133-138 |
FW |
denotes |
vitro |
T7655 |
163-165 |
VBZ |
denotes |
is |
T7656 |
139-144 |
DT |
denotes |
these |
T7657 |
153-162 |
NN |
denotes |
formation |
T7658 |
145-152 |
NN |
denotes |
vesicle |
T7659 |
166-170 |
NN |
denotes |
Ca2+ |
T7660 |
171-178 |
VBN |
denotes |
induced |
T7661 |
178-179 |
. |
denotes |
. |
T7662 |
179-293 |
sentence |
denotes |
Freshly collected lithium-heparin anticoagulated blood samples were centrifuged for 5 minutes at 400 × g and 4°C. |
T7663 |
180-187 |
RB |
denotes |
Freshly |
T7664 |
188-197 |
VBN |
denotes |
collected |
T7665 |
235-242 |
NNS |
denotes |
samples |
T7666 |
198-205 |
NN |
denotes |
lithium |
T7667 |
206-213 |
NN |
denotes |
heparin |
T7668 |
205-206 |
HYPH |
denotes |
- |
T7669 |
214-228 |
VBN |
denotes |
anticoagulated |
T7670 |
229-234 |
NN |
denotes |
blood |
T7671 |
248-259 |
VBN |
denotes |
centrifuged |
T7672 |
243-247 |
VBD |
denotes |
were |
T7673 |
260-263 |
IN |
denotes |
for |
T7674 |
264-265 |
CD |
denotes |
5 |
T7675 |
266-273 |
NNS |
denotes |
minutes |
T7676 |
274-276 |
IN |
denotes |
at |
T7677 |
277-280 |
CD |
denotes |
400 |
T7678 |
283-284 |
NN |
denotes |
g |
T7679 |
281-282 |
SYM |
denotes |
× |
T7680 |
285-288 |
CC |
denotes |
and |
T7681 |
289-290 |
CD |
denotes |
4 |
T7682 |
290-292 |
NN |
denotes |
°C |
T7683 |
292-293 |
. |
denotes |
. |
T7684 |
293-393 |
sentence |
denotes |
The erythrocytes were washed five times with 20 mM Hepes, pH 7.5, and 150 mM NaCl (washing buffer). |
T7685 |
294-297 |
DT |
denotes |
The |
T7686 |
298-310 |
NNS |
denotes |
erythrocytes |
T7687 |
316-322 |
VBN |
denotes |
washed |
T7688 |
311-315 |
VBD |
denotes |
were |
T7689 |
323-327 |
CD |
denotes |
five |
T7690 |
328-333 |
NNS |
denotes |
times |
T7691 |
334-338 |
IN |
denotes |
with |
T7692 |
339-341 |
CD |
denotes |
20 |
T7693 |
342-344 |
NN |
denotes |
mM |
T7694 |
345-350 |
NN |
denotes |
Hepes |
T7695 |
350-352 |
, |
denotes |
, |
T7696 |
352-354 |
NN |
denotes |
pH |
T7697 |
355-358 |
CD |
denotes |
7.5 |
T7698 |
358-360 |
, |
denotes |
, |
T7699 |
360-363 |
CC |
denotes |
and |
T7700 |
364-367 |
CD |
denotes |
150 |
T7701 |
368-370 |
NN |
denotes |
mM |
T7702 |
371-375 |
NN |
denotes |
NaCl |
T7703 |
376-377 |
-LRB- |
denotes |
( |
T7704 |
385-391 |
NN |
denotes |
buffer |
T7705 |
377-384 |
VBG |
denotes |
washing |
T7706 |
391-392 |
-RRB- |
denotes |
) |
T7707 |
392-393 |
. |
denotes |
. |
T7708 |
393-606 |
sentence |
denotes |
A volume of 500 μl washed red blood cells were combined with 3 ml of vesiculation buffer (20 mM Hepes, pH 7.5, 150 mM NaCl, 1 mM CaCl2 adjusted to 5 μM Ca2+ ionophore A23187) and incubated for 30 minutes at 37°C. |
T7709 |
394-395 |
DT |
denotes |
A |
T7710 |
396-402 |
NN |
denotes |
volume |
T7711 |
441-449 |
VBN |
denotes |
combined |
T7712 |
403-405 |
IN |
denotes |
of |
T7713 |
406-409 |
CD |
denotes |
500 |
T7714 |
410-412 |
NN |
denotes |
μl |
T7715 |
430-435 |
NNS |
denotes |
cells |
T7716 |
413-419 |
VBN |
denotes |
washed |
T7717 |
420-423 |
JJ |
denotes |
red |
T7718 |
424-429 |
NN |
denotes |
blood |
T7719 |
436-440 |
VBD |
denotes |
were |
T7720 |
450-454 |
IN |
denotes |
with |
T7721 |
455-456 |
CD |
denotes |
3 |
T7722 |
457-459 |
NN |
denotes |
ml |
T7723 |
460-462 |
IN |
denotes |
of |
T7724 |
463-475 |
NN |
denotes |
vesiculation |
T7725 |
476-482 |
NN |
denotes |
buffer |
T7726 |
483-484 |
-LRB- |
denotes |
( |
T7727 |
523-528 |
NN |
denotes |
CaCl2 |
T7728 |
484-486 |
CD |
denotes |
20 |
T7729 |
487-489 |
NN |
denotes |
mM |
T7730 |
490-495 |
NN |
denotes |
Hepes |
T7731 |
495-497 |
, |
denotes |
, |
T7732 |
497-499 |
NN |
denotes |
pH |
T7733 |
500-503 |
CD |
denotes |
7.5 |
T7734 |
503-505 |
, |
denotes |
, |
T7735 |
505-508 |
CD |
denotes |
150 |
T7736 |
509-511 |
NN |
denotes |
mM |
T7737 |
512-516 |
NN |
denotes |
NaCl |
T7738 |
516-518 |
, |
denotes |
, |
T7739 |
518-519 |
CD |
denotes |
1 |
T7740 |
520-522 |
NN |
denotes |
mM |
T7741 |
529-537 |
VBN |
denotes |
adjusted |
T7742 |
538-540 |
IN |
denotes |
to |
T7743 |
541-542 |
CD |
denotes |
5 |
T7744 |
543-545 |
NN |
denotes |
μM |
T7745 |
561-567 |
NN |
denotes |
A23187 |
T7746 |
546-550 |
NN |
denotes |
Ca2+ |
T7747 |
551-560 |
NN |
denotes |
ionophore |
T7748 |
567-568 |
-RRB- |
denotes |
) |
T7749 |
569-572 |
CC |
denotes |
and |
T7750 |
573-582 |
VBN |
denotes |
incubated |
T7751 |
583-586 |
IN |
denotes |
for |
T7752 |
587-589 |
CD |
denotes |
30 |
T7753 |
590-597 |
NNS |
denotes |
minutes |
T7754 |
598-600 |
IN |
denotes |
at |
T7755 |
601-603 |
CD |
denotes |
37 |
T7756 |
603-605 |
NNS |
denotes |
°C |
T7757 |
605-606 |
. |
denotes |
. |
T7758 |
606-737 |
sentence |
denotes |
After incubation EDTA was added to the solution to a final concentration of 5 mM and centrifuged for 5 minutes at 400 × g and 4°C. |
T7759 |
607-612 |
IN |
denotes |
After |
T7760 |
633-638 |
VBN |
denotes |
added |
T7761 |
613-623 |
NN |
denotes |
incubation |
T7762 |
624-628 |
NN |
denotes |
EDTA |
T7763 |
629-632 |
VBD |
denotes |
was |
T7764 |
639-641 |
IN |
denotes |
to |
T7765 |
642-645 |
DT |
denotes |
the |
T7766 |
646-654 |
NN |
denotes |
solution |
T7767 |
655-657 |
IN |
denotes |
to |
T7768 |
658-659 |
DT |
denotes |
a |
T7769 |
666-679 |
NN |
denotes |
concentration |
T7770 |
660-665 |
JJ |
denotes |
final |
T7771 |
680-682 |
IN |
denotes |
of |
T7772 |
683-684 |
CD |
denotes |
5 |
T7773 |
685-687 |
NN |
denotes |
mM |
T7774 |
688-691 |
CC |
denotes |
and |
T7775 |
692-703 |
VBN |
denotes |
centrifuged |
T7776 |
704-707 |
IN |
denotes |
for |
T7777 |
708-709 |
CD |
denotes |
5 |
T7778 |
710-717 |
NNS |
denotes |
minutes |
T7779 |
718-720 |
IN |
denotes |
at |
T7780 |
721-724 |
CD |
denotes |
400 |
T7781 |
727-728 |
NN |
denotes |
g |
T7782 |
725-726 |
SYM |
denotes |
× |
T7783 |
729-732 |
CC |
denotes |
and |
T7784 |
733-734 |
CD |
denotes |
4 |
T7785 |
734-736 |
NNS |
denotes |
°C |
T7786 |
736-737 |
. |
denotes |
. |
T7787 |
737-821 |
sentence |
denotes |
The supernatant was collected and centrifuged for 20 minutes at 15,000 × g and 4°C. |
T7788 |
738-741 |
DT |
denotes |
The |
T7789 |
742-753 |
NN |
denotes |
supernatant |
T7790 |
758-767 |
VBN |
denotes |
collected |
T7791 |
754-757 |
VBD |
denotes |
was |
T7792 |
768-771 |
CC |
denotes |
and |
T7793 |
772-783 |
VBN |
denotes |
centrifuged |
T7794 |
784-787 |
IN |
denotes |
for |
T7795 |
788-790 |
CD |
denotes |
20 |
T7796 |
791-798 |
NNS |
denotes |
minutes |
T7797 |
799-801 |
IN |
denotes |
at |
T7798 |
802-808 |
CD |
denotes |
15,000 |
T7799 |
811-812 |
NN |
denotes |
g |
T7800 |
809-810 |
SYM |
denotes |
× |
T7801 |
813-816 |
CC |
denotes |
and |
T7802 |
817-818 |
CD |
denotes |
4 |
T7803 |
818-820 |
NNS |
denotes |
°C |
T7804 |
820-821 |
. |
denotes |
. |
T7805 |
821-978 |
sentence |
denotes |
The resulting pellet was resuspended in washing buffer and centrifuged at 400 × g again to collect a supernatant enriched with red blood cell microvesicles. |
T7806 |
822-825 |
DT |
denotes |
The |
T7807 |
836-842 |
NN |
denotes |
pellet |
T7808 |
826-835 |
VBG |
denotes |
resulting |
T7809 |
847-858 |
VBN |
denotes |
resuspended |
T7810 |
843-846 |
VBD |
denotes |
was |
T7811 |
859-861 |
IN |
denotes |
in |
T7812 |
862-869 |
VBG |
denotes |
washing |
T7813 |
870-876 |
NN |
denotes |
buffer |
T7814 |
877-880 |
CC |
denotes |
and |
T7815 |
881-892 |
VBN |
denotes |
centrifuged |
T7816 |
893-895 |
IN |
denotes |
at |
T7817 |
896-899 |
CD |
denotes |
400 |
T7818 |
902-903 |
NN |
denotes |
g |
T7819 |
900-901 |
SYM |
denotes |
× |
T7820 |
904-909 |
RB |
denotes |
again |
T7821 |
910-912 |
TO |
denotes |
to |
T7822 |
913-920 |
VB |
denotes |
collect |
T7823 |
921-922 |
DT |
denotes |
a |
T7824 |
923-934 |
NN |
denotes |
supernatant |
T7825 |
935-943 |
VBN |
denotes |
enriched |
T7826 |
944-948 |
IN |
denotes |
with |
T7827 |
949-952 |
JJ |
denotes |
red |
T7828 |
959-963 |
NN |
denotes |
cell |
T7829 |
953-958 |
NN |
denotes |
blood |
T7830 |
964-977 |
NNS |
denotes |
microvesicles |
T7831 |
977-978 |
. |
denotes |
. |
T7832 |
978-1080 |
sentence |
denotes |
The supernatant of the 15,000 × g step was further centrifuged for 60 minutes at 100,000 × g and 4°C. |
T7833 |
979-982 |
DT |
denotes |
The |
T7834 |
983-994 |
NN |
denotes |
supernatant |
T7835 |
1030-1041 |
VBN |
denotes |
centrifuged |
T7836 |
995-997 |
IN |
denotes |
of |
T7837 |
998-1001 |
DT |
denotes |
the |
T7838 |
1013-1017 |
NN |
denotes |
step |
T7839 |
1002-1008 |
CD |
denotes |
15,000 |
T7840 |
1011-1012 |
NN |
denotes |
g |
T7841 |
1009-1010 |
SYM |
denotes |
× |
T7842 |
1018-1021 |
VBD |
denotes |
was |
T7843 |
1022-1029 |
RBR |
denotes |
further |
T7844 |
1042-1045 |
IN |
denotes |
for |
T7845 |
1046-1048 |
CD |
denotes |
60 |
T7846 |
1049-1056 |
NNS |
denotes |
minutes |
T7847 |
1057-1059 |
IN |
denotes |
at |
T7848 |
1060-1067 |
CD |
denotes |
100,000 |
T7849 |
1070-1071 |
NN |
denotes |
g |
T7850 |
1068-1069 |
SYM |
denotes |
× |
T7851 |
1072-1075 |
CC |
denotes |
and |
T7852 |
1076-1077 |
CD |
denotes |
4 |
T7853 |
1077-1079 |
NNS |
denotes |
°C |
T7854 |
1079-1080 |
. |
denotes |
. |
T7855 |
1080-1250 |
sentence |
denotes |
The final pellet of this step was resuspended in washing buffer and centrifuged at 15,000 × g again to purify a supernatant enriched in nanovesicles according to [7,20]. |
T7856 |
1081-1084 |
DT |
denotes |
The |
T7857 |
1091-1097 |
NN |
denotes |
pellet |
T7858 |
1085-1090 |
JJ |
denotes |
final |
T7859 |
1115-1126 |
VBN |
denotes |
resuspended |
T7860 |
1098-1100 |
IN |
denotes |
of |
T7861 |
1101-1105 |
DT |
denotes |
this |
T7862 |
1106-1110 |
NN |
denotes |
step |
T7863 |
1111-1114 |
VBD |
denotes |
was |
T7864 |
1127-1129 |
IN |
denotes |
in |
T7865 |
1130-1137 |
VBG |
denotes |
washing |
T7866 |
1138-1144 |
NN |
denotes |
buffer |
T7867 |
1145-1148 |
CC |
denotes |
and |
T7868 |
1149-1160 |
VBN |
denotes |
centrifuged |
T7869 |
1161-1163 |
IN |
denotes |
at |
T7870 |
1164-1170 |
CD |
denotes |
15,000 |
T7871 |
1173-1174 |
NN |
denotes |
g |
T7872 |
1171-1172 |
SYM |
denotes |
× |
T7873 |
1175-1180 |
RB |
denotes |
again |
T7874 |
1181-1183 |
TO |
denotes |
to |
T7875 |
1184-1190 |
VB |
denotes |
purify |
T7876 |
1191-1192 |
DT |
denotes |
a |
T7877 |
1193-1204 |
NN |
denotes |
supernatant |
T7878 |
1205-1213 |
VBN |
denotes |
enriched |
T7879 |
1214-1216 |
IN |
denotes |
in |
T7880 |
1217-1229 |
NNS |
denotes |
nanovesicles |
T7881 |
1230-1239 |
VBG |
denotes |
according |
T7882 |
1240-1242 |
IN |
denotes |
to |
T7883 |
1243-1244 |
-LRB- |
denotes |
[ |
T7884 |
1244-1245 |
CD |
denotes |
7 |
T7885 |
1246-1248 |
CD |
denotes |
20 |
T7886 |
1245-1246 |
, |
denotes |
, |
T7887 |
1248-1249 |
-RRB- |
denotes |
] |
T7888 |
1249-1250 |
. |
denotes |
. |
T7889 |
1250-1343 |
sentence |
denotes |
The vesicles were assayed for acetylcholine esterase activity and used for further analysis. |
T7890 |
1251-1254 |
DT |
denotes |
The |
T7891 |
1255-1263 |
NNS |
denotes |
vesicles |
T7892 |
1269-1276 |
VBN |
denotes |
assayed |
T7893 |
1264-1268 |
VBD |
denotes |
were |
T7894 |
1277-1280 |
IN |
denotes |
for |
T7895 |
1281-1294 |
NN |
denotes |
acetylcholine |
T7896 |
1295-1303 |
NN |
denotes |
esterase |
T7897 |
1304-1312 |
NN |
denotes |
activity |
T7898 |
1313-1316 |
CC |
denotes |
and |
T7899 |
1317-1321 |
VBN |
denotes |
used |
T7900 |
1322-1325 |
IN |
denotes |
for |
T7901 |
1326-1333 |
JJ |
denotes |
further |
T7902 |
1334-1342 |
NN |
denotes |
analysis |
T7903 |
1342-1343 |
. |
denotes |
. |
R4664 |
T7632 |
T7633 |
compound |
Exovesicle,generation |
R4665 |
T7634 |
T7633 |
prep |
from,generation |
R4666 |
T7635 |
T7636 |
amod |
human,cells |
R4667 |
T7636 |
T7634 |
pobj |
cells,from |
R4668 |
T7637 |
T7635 |
cc |
and,human |
R4669 |
T7638 |
T7635 |
conj |
murine,human |
R4670 |
T7639 |
T7636 |
amod |
red,cells |
R4671 |
T7640 |
T7636 |
compound |
blood,cells |
R4672 |
T7642 |
T7643 |
amod |
Red,cells |
R4673 |
T7643 |
T7645 |
nsubj |
cells,form |
R4674 |
T7644 |
T7643 |
compound |
blood,cells |
R4675 |
T7646 |
T7645 |
advmod |
naturally,form |
R4676 |
T7647 |
T7645 |
aux |
can,form |
R4677 |
T7648 |
T7649 |
npadvmod |
hemoglobin,containing |
R4678 |
T7649 |
T7650 |
amod |
containing,exovesicles |
R4679 |
T7650 |
T7645 |
dobj |
exovesicles,form |
R4680 |
T7651 |
T7645 |
punct |
.,form |
R4681 |
T7653 |
T7654 |
advmod |
In,vitro |
R4682 |
T7654 |
T7655 |
advmod |
vitro,is |
R4683 |
T7656 |
T7657 |
det |
these,formation |
R4684 |
T7657 |
T7655 |
nsubj |
formation,is |
R4685 |
T7658 |
T7657 |
compound |
vesicle,formation |
R4686 |
T7659 |
T7660 |
npadvmod |
Ca2+,induced |
R4687 |
T7660 |
T7655 |
acomp |
induced,is |
R4688 |
T7661 |
T7655 |
punct |
.,is |
R4689 |
T7663 |
T7664 |
advmod |
Freshly,collected |
R4690 |
T7664 |
T7665 |
amod |
collected,samples |
R4691 |
T7665 |
T7671 |
nsubjpass |
samples,centrifuged |
R4692 |
T7666 |
T7667 |
nmod |
lithium,heparin |
R4693 |
T7667 |
T7665 |
nmod |
heparin,samples |
R4694 |
T7668 |
T7667 |
punct |
-,heparin |
R4695 |
T7669 |
T7665 |
amod |
anticoagulated,samples |
R4696 |
T7670 |
T7665 |
compound |
blood,samples |
R4697 |
T7672 |
T7671 |
auxpass |
were,centrifuged |
R4698 |
T7673 |
T7671 |
prep |
for,centrifuged |
R4699 |
T7674 |
T7675 |
nummod |
5,minutes |
R4700 |
T7675 |
T7673 |
pobj |
minutes,for |
R4701 |
T7676 |
T7671 |
prep |
at,centrifuged |
R4702 |
T7677 |
T7678 |
nummod |
400,g |
R4703 |
T7678 |
T7676 |
pobj |
g,at |
R4704 |
T7679 |
T7678 |
punct |
×,g |
R4705 |
T7680 |
T7678 |
cc |
and,g |
R4706 |
T7681 |
T7682 |
nummod |
4,°C |
R4707 |
T7682 |
T7678 |
conj |
°C,g |
R4708 |
T7683 |
T7671 |
punct |
.,centrifuged |
R4709 |
T7685 |
T7686 |
det |
The,erythrocytes |
R4710 |
T7686 |
T7687 |
nsubjpass |
erythrocytes,washed |
R4711 |
T7688 |
T7687 |
auxpass |
were,washed |
R4712 |
T7689 |
T7690 |
nummod |
five,times |
R4713 |
T7690 |
T7687 |
npadvmod |
times,washed |
R4714 |
T7691 |
T7687 |
prep |
with,washed |
R4715 |
T7692 |
T7693 |
nummod |
20,mM |
R4716 |
T7693 |
T7694 |
compound |
mM,Hepes |
R4717 |
T7694 |
T7691 |
pobj |
Hepes,with |
R4718 |
T7695 |
T7694 |
punct |
", ",Hepes |
R4719 |
T7696 |
T7694 |
npadvmod |
pH,Hepes |
R4720 |
T7697 |
T7696 |
nummod |
7.5,pH |
R4721 |
T7698 |
T7694 |
punct |
", ",Hepes |
R4722 |
T7699 |
T7694 |
cc |
and,Hepes |
R4723 |
T7700 |
T7701 |
nummod |
150,mM |
R4724 |
T7701 |
T7702 |
compound |
mM,NaCl |
R4725 |
T7702 |
T7694 |
conj |
NaCl,Hepes |
R4726 |
T7703 |
T7704 |
punct |
(,buffer |
R4727 |
T7704 |
T7702 |
parataxis |
buffer,NaCl |
R4728 |
T7705 |
T7704 |
amod |
washing,buffer |
R4729 |
T7706 |
T7704 |
punct |
),buffer |
R4730 |
T7707 |
T7687 |
punct |
.,washed |
R4731 |
T7709 |
T7710 |
det |
A,volume |
R4732 |
T7710 |
T7711 |
nsubjpass |
volume,combined |
R4733 |
T7712 |
T7710 |
prep |
of,volume |
R4734 |
T7713 |
T7714 |
nummod |
500,μl |
R4735 |
T7714 |
T7715 |
nmod |
μl,cells |
R4736 |
T7715 |
T7712 |
pobj |
cells,of |
R4737 |
T7716 |
T7715 |
amod |
washed,cells |
R4738 |
T7717 |
T7715 |
amod |
red,cells |
R4739 |
T7718 |
T7715 |
compound |
blood,cells |
R4740 |
T7719 |
T7711 |
auxpass |
were,combined |
R4741 |
T7720 |
T7711 |
prep |
with,combined |
R4742 |
T7721 |
T7722 |
nummod |
3,ml |
R4743 |
T7722 |
T7720 |
pobj |
ml,with |
R4744 |
T7723 |
T7722 |
prep |
of,ml |
R4745 |
T7724 |
T7725 |
compound |
vesiculation,buffer |
R4746 |
T7725 |
T7723 |
pobj |
buffer,of |
R4747 |
T7726 |
T7727 |
punct |
(,CaCl2 |
R4748 |
T7727 |
T7725 |
parataxis |
CaCl2,buffer |
R4749 |
T7728 |
T7729 |
nummod |
20,mM |
R4750 |
T7729 |
T7730 |
compound |
mM,Hepes |
R4751 |
T7730 |
T7727 |
dep |
Hepes,CaCl2 |
R4752 |
T7731 |
T7730 |
punct |
", ",Hepes |
R4753 |
T7732 |
T7730 |
npadvmod |
pH,Hepes |
R4754 |
T7733 |
T7732 |
nummod |
7.5,pH |
R4755 |
T7734 |
T7727 |
punct |
", ",CaCl2 |
R4756 |
T7735 |
T7736 |
nummod |
150,mM |
R4757 |
T7736 |
T7737 |
compound |
mM,NaCl |
R4758 |
T7737 |
T7727 |
dep |
NaCl,CaCl2 |
R4759 |
T7738 |
T7727 |
punct |
", ",CaCl2 |
R4760 |
T7739 |
T7740 |
nummod |
1,mM |
R4761 |
T7740 |
T7727 |
compound |
mM,CaCl2 |
R4762 |
T7741 |
T7727 |
acl |
adjusted,CaCl2 |
R4763 |
T7742 |
T7741 |
prep |
to,adjusted |
R4764 |
T7743 |
T7744 |
nummod |
5,μM |
R4765 |
T7744 |
T7745 |
compound |
μM,A23187 |
R4766 |
T7745 |
T7742 |
pobj |
A23187,to |
R4767 |
T7746 |
T7745 |
compound |
Ca2+,A23187 |
R4768 |
T7747 |
T7745 |
compound |
ionophore,A23187 |
R4769 |
T7748 |
T7727 |
punct |
),CaCl2 |
R4770 |
T7749 |
T7711 |
cc |
and,combined |
R4771 |
T7750 |
T7711 |
conj |
incubated,combined |
R4772 |
T7751 |
T7750 |
prep |
for,incubated |
R4773 |
T7752 |
T7753 |
nummod |
30,minutes |
R4774 |
T7753 |
T7751 |
pobj |
minutes,for |
R4775 |
T7754 |
T7750 |
prep |
at,incubated |
R4776 |
T7755 |
T7756 |
nummod |
37,°C |
R4777 |
T7756 |
T7754 |
pobj |
°C,at |
R4778 |
T7757 |
T7711 |
punct |
.,combined |
R4779 |
T7759 |
T7760 |
prep |
After,added |
R4780 |
T7761 |
T7759 |
pobj |
incubation,After |
R4781 |
T7762 |
T7760 |
nsubjpass |
EDTA,added |
R4782 |
T7763 |
T7760 |
auxpass |
was,added |
R4783 |
T7764 |
T7760 |
prep |
to,added |
R4784 |
T7765 |
T7766 |
det |
the,solution |
R4785 |
T7766 |
T7764 |
pobj |
solution,to |
R4786 |
T7767 |
T7760 |
prep |
to,added |
R4787 |
T7768 |
T7769 |
det |
a,concentration |
R4788 |
T7769 |
T7767 |
pobj |
concentration,to |
R4789 |
T7770 |
T7769 |
amod |
final,concentration |
R4790 |
T7771 |
T7769 |
prep |
of,concentration |
R4791 |
T7772 |
T7773 |
nummod |
5,mM |
R4792 |
T7773 |
T7771 |
pobj |
mM,of |
R4793 |
T7774 |
T7760 |
cc |
and,added |
R4794 |
T7775 |
T7760 |
conj |
centrifuged,added |
R4795 |
T7776 |
T7775 |
prep |
for,centrifuged |
R4796 |
T7777 |
T7778 |
nummod |
5,minutes |
R4797 |
T7778 |
T7776 |
pobj |
minutes,for |
R4798 |
T7779 |
T7775 |
prep |
at,centrifuged |
R4799 |
T7780 |
T7781 |
nummod |
400,g |
R4800 |
T7781 |
T7779 |
pobj |
g,at |
R4801 |
T7782 |
T7781 |
punct |
×,g |
R4802 |
T7783 |
T7781 |
cc |
and,g |
R4803 |
T7784 |
T7785 |
nummod |
4,°C |
R4804 |
T7785 |
T7781 |
conj |
°C,g |
R4805 |
T7786 |
T7760 |
punct |
.,added |
R4806 |
T7788 |
T7789 |
det |
The,supernatant |
R4807 |
T7789 |
T7790 |
nsubjpass |
supernatant,collected |
R4808 |
T7791 |
T7790 |
auxpass |
was,collected |
R4809 |
T7792 |
T7790 |
cc |
and,collected |
R4810 |
T7793 |
T7790 |
conj |
centrifuged,collected |
R4811 |
T7794 |
T7793 |
prep |
for,centrifuged |
R4812 |
T7795 |
T7796 |
nummod |
20,minutes |
R4813 |
T7796 |
T7794 |
pobj |
minutes,for |
R4814 |
T7797 |
T7793 |
prep |
at,centrifuged |
R4815 |
T7798 |
T7799 |
nummod |
"15,000",g |
R4816 |
T7799 |
T7797 |
pobj |
g,at |
R4817 |
T7800 |
T7799 |
punct |
×,g |
R4818 |
T7801 |
T7799 |
cc |
and,g |
R4819 |
T7802 |
T7803 |
nummod |
4,°C |
R4820 |
T7803 |
T7799 |
conj |
°C,g |
R4821 |
T7804 |
T7790 |
punct |
.,collected |
R4822 |
T7806 |
T7807 |
det |
The,pellet |
R4823 |
T7807 |
T7809 |
nsubj |
pellet,resuspended |
R4824 |
T7808 |
T7807 |
amod |
resulting,pellet |
R4825 |
T7810 |
T7809 |
aux |
was,resuspended |
R4826 |
T7811 |
T7809 |
prep |
in,resuspended |
R4827 |
T7812 |
T7813 |
amod |
washing,buffer |
R4828 |
T7813 |
T7811 |
pobj |
buffer,in |
R4829 |
T7814 |
T7809 |
cc |
and,resuspended |
R4830 |
T7815 |
T7809 |
conj |
centrifuged,resuspended |
R4831 |
T7816 |
T7815 |
prep |
at,centrifuged |
R4832 |
T7817 |
T7818 |
nummod |
400,g |
R4833 |
T7818 |
T7816 |
pobj |
g,at |
R4834 |
T7819 |
T7818 |
punct |
×,g |
R4835 |
T7820 |
T7815 |
advmod |
again,centrifuged |
R4836 |
T7821 |
T7822 |
aux |
to,collect |
R4837 |
T7822 |
T7809 |
advcl |
collect,resuspended |
R4838 |
T7823 |
T7824 |
det |
a,supernatant |
R4839 |
T7824 |
T7822 |
dobj |
supernatant,collect |
R4840 |
T7825 |
T7824 |
acl |
enriched,supernatant |
R4841 |
T7826 |
T7825 |
prep |
with,enriched |
R4842 |
T7827 |
T7828 |
amod |
red,cell |
R4843 |
T7828 |
T7830 |
compound |
cell,microvesicles |
R4844 |
T7829 |
T7828 |
compound |
blood,cell |
R4845 |
T7830 |
T7826 |
pobj |
microvesicles,with |
R4846 |
T7831 |
T7809 |
punct |
.,resuspended |
R4847 |
T7833 |
T7834 |
det |
The,supernatant |
R4848 |
T7834 |
T7835 |
nsubjpass |
supernatant,centrifuged |
R4849 |
T7836 |
T7834 |
prep |
of,supernatant |
R4850 |
T7837 |
T7838 |
det |
the,step |
R4851 |
T7838 |
T7836 |
pobj |
step,of |
R4852 |
T7839 |
T7840 |
nummod |
"15,000",g |
R4853 |
T7840 |
T7838 |
compound |
g,step |
R4854 |
T7841 |
T7840 |
punct |
×,g |
R4855 |
T7842 |
T7835 |
auxpass |
was,centrifuged |
R4856 |
T7843 |
T7835 |
advmod |
further,centrifuged |
R4857 |
T7844 |
T7835 |
prep |
for,centrifuged |
R4858 |
T7845 |
T7846 |
nummod |
60,minutes |
R4859 |
T7846 |
T7844 |
pobj |
minutes,for |
R4860 |
T7847 |
T7835 |
prep |
at,centrifuged |
R4861 |
T7848 |
T7849 |
nummod |
"100,000",g |
R4862 |
T7849 |
T7847 |
pobj |
g,at |
R4863 |
T7850 |
T7849 |
punct |
×,g |
R4864 |
T7851 |
T7849 |
cc |
and,g |
R4865 |
T7852 |
T7853 |
nummod |
4,°C |
R4866 |
T7853 |
T7849 |
conj |
°C,g |
R4867 |
T7854 |
T7835 |
punct |
.,centrifuged |
R4868 |
T7856 |
T7857 |
det |
The,pellet |
R4869 |
T7857 |
T7859 |
nsubj |
pellet,resuspended |
R4870 |
T7858 |
T7857 |
amod |
final,pellet |
R4871 |
T7860 |
T7857 |
prep |
of,pellet |
R4872 |
T7861 |
T7862 |
det |
this,step |
R4873 |
T7862 |
T7860 |
pobj |
step,of |
R4874 |
T7863 |
T7859 |
aux |
was,resuspended |
R4875 |
T7864 |
T7859 |
prep |
in,resuspended |
R4876 |
T7865 |
T7866 |
amod |
washing,buffer |
R4877 |
T7866 |
T7864 |
pobj |
buffer,in |
R4878 |
T7867 |
T7859 |
cc |
and,resuspended |
R4879 |
T7868 |
T7859 |
conj |
centrifuged,resuspended |
R4880 |
T7869 |
T7868 |
prep |
at,centrifuged |
R4881 |
T7870 |
T7871 |
nummod |
"15,000",g |
R4882 |
T7871 |
T7869 |
pobj |
g,at |
R4883 |
T7872 |
T7871 |
punct |
×,g |
R4884 |
T7873 |
T7868 |
advmod |
again,centrifuged |
R4885 |
T7874 |
T7875 |
aux |
to,purify |
R4886 |
T7875 |
T7859 |
advcl |
purify,resuspended |
R4887 |
T7876 |
T7877 |
det |
a,supernatant |
R4888 |
T7877 |
T7875 |
dobj |
supernatant,purify |
R4889 |
T7878 |
T7877 |
acl |
enriched,supernatant |
R4890 |
T7879 |
T7878 |
prep |
in,enriched |
R4891 |
T7880 |
T7879 |
pobj |
nanovesicles,in |
R4892 |
T7881 |
T7875 |
prep |
according,purify |
R4893 |
T7882 |
T7881 |
prep |
to,according |
R4894 |
T7883 |
T7882 |
punct |
[,to |
R4895 |
T7884 |
T7885 |
nummod |
7,20 |
R4896 |
T7885 |
T7882 |
pobj |
20,to |
R4897 |
T7886 |
T7885 |
punct |
",",20 |
R4898 |
T7887 |
T7859 |
punct |
],resuspended |
R4899 |
T7888 |
T7859 |
punct |
.,resuspended |
R4900 |
T7890 |
T7891 |
det |
The,vesicles |
R4901 |
T7891 |
T7892 |
nsubjpass |
vesicles,assayed |
R4902 |
T7893 |
T7892 |
auxpass |
were,assayed |
R4903 |
T7894 |
T7892 |
prep |
for,assayed |
R4904 |
T7895 |
T7896 |
compound |
acetylcholine,esterase |
R4905 |
T7896 |
T7897 |
compound |
esterase,activity |
R4906 |
T7897 |
T7894 |
pobj |
activity,for |
R4907 |
T7898 |
T7892 |
cc |
and,assayed |
R4908 |
T7899 |
T7892 |
conj |
used,assayed |
R4909 |
T7900 |
T7899 |
prep |
for,used |
R4910 |
T7901 |
T7902 |
amod |
further,analysis |
R4911 |
T7902 |
T7900 |
pobj |
analysis,for |
R4912 |
T7903 |
T7892 |
punct |
.,assayed |