PMC:194730 / 28839-29145
Annnotations
craft-sa-dev
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Red blood cells were centrifuged and washed in isotonic buffered salt solution, collected in ice cold lysis buffer (20 mM Hepes, pH 7.4, 200 μM CaCl2, 0.25 M sucrose, and proteinase inhibitors (Roche)), homogenized by a loosely fitting dounce homogenizer and additional passages through a 22 gauge needle."}
craft-ca-core-dev
{"project":"craft-ca-core-dev","denotations":[{"id":"T7212","span":{"begin":1,"end":16},"obj":"CL:0000232"},{"id":"T7213","span":{"begin":5,"end":10},"obj":"UBERON:0000178"},{"id":"T7214","span":{"begin":103,"end":108},"obj":"GO:0019835"},{"id":"T7215","span":{"begin":123,"end":128},"obj":"CHEBI:46756"},{"id":"T7216","span":{"begin":145,"end":150},"obj":"CHEBI:3312"},{"id":"T7217","span":{"begin":159,"end":166},"obj":"CHEBI:17992"}],"text":" Red blood cells were centrifuged and washed in isotonic buffered salt solution, collected in ice cold lysis buffer (20 mM Hepes, pH 7.4, 200 μM CaCl2, 0.25 M sucrose, and proteinase inhibitors (Roche)), homogenized by a loosely fitting dounce homogenizer and additional passages through a 22 gauge needle."}
craft-ca-core-ex-dev
{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T7242","span":{"begin":1,"end":16},"obj":"CL:0000232"},{"id":"T7243","span":{"begin":5,"end":10},"obj":"UBERON:0000178"},{"id":"T7244","span":{"begin":11,"end":16},"obj":"CL_GO_EXT:cell"},{"id":"T7245","span":{"begin":57,"end":65},"obj":"CHEBI_CHMO_EXT:buffer_process"},{"id":"T7246","span":{"begin":66,"end":79},"obj":"CHEBI_EXT:saline_solution"},{"id":"T7247","span":{"begin":103,"end":108},"obj":"GO:0019835"},{"id":"T7248","span":{"begin":109,"end":115},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T7249","span":{"begin":123,"end":128},"obj":"CHEBI:46756"},{"id":"T7250","span":{"begin":145,"end":150},"obj":"CHEBI:3312"},{"id":"T7251","span":{"begin":159,"end":166},"obj":"CHEBI:17992"},{"id":"T7252","span":{"begin":172,"end":193},"obj":"CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor"},{"id":"T7253","span":{"begin":271,"end":279},"obj":"GO_EXT:biological_movement_or_translocation_process"}],"text":" Red blood cells were centrifuged and washed in isotonic buffered salt solution, collected in ice cold lysis buffer (20 mM Hepes, pH 7.4, 200 μM CaCl2, 0.25 M sucrose, and proteinase inhibitors (Roche)), homogenized by a loosely fitting dounce homogenizer and additional passages through a 22 gauge needle."}