Id |
Subject |
Object |
Predicate |
Lexical cue |
T7284 |
0-3 |
DT |
denotes |
The |
T7285 |
16-28 |
NN |
denotes |
distribution |
T7286 |
4-15 |
JJ |
denotes |
subcellular |
T7287 |
47-56 |
VBN |
denotes |
addressed |
T7288 |
29-31 |
IN |
denotes |
of |
T7289 |
32-39 |
NN |
denotes |
annexin |
T7290 |
40-42 |
NN |
denotes |
A7 |
T7291 |
43-46 |
VBD |
denotes |
was |
T7292 |
57-66 |
VBG |
denotes |
according |
T7293 |
67-69 |
IN |
denotes |
to |
T7294 |
70-71 |
-LRB- |
denotes |
[ |
T7295 |
71-73 |
CD |
denotes |
24 |
T7296 |
73-74 |
-RRB- |
denotes |
] |
T7297 |
74-75 |
. |
denotes |
. |
T7298 |
75-381 |
sentence |
denotes |
Red blood cells were centrifuged and washed in isotonic buffered salt solution, collected in ice cold lysis buffer (20 mM Hepes, pH 7.4, 200 μM CaCl2, 0.25 M sucrose, and proteinase inhibitors (Roche)), homogenized by a loosely fitting dounce homogenizer and additional passages through a 22 gauge needle. |
T7299 |
76-79 |
JJ |
denotes |
Red |
T7300 |
86-91 |
NNS |
denotes |
cells |
T7301 |
80-85 |
NN |
denotes |
blood |
T7302 |
97-108 |
VBN |
denotes |
centrifuged |
T7303 |
92-96 |
VBD |
denotes |
were |
T7304 |
109-112 |
CC |
denotes |
and |
T7305 |
113-119 |
VBN |
denotes |
washed |
T7306 |
120-122 |
IN |
denotes |
in |
T7307 |
123-131 |
JJ |
denotes |
isotonic |
T7308 |
146-154 |
NN |
denotes |
solution |
T7309 |
132-140 |
VBN |
denotes |
buffered |
T7310 |
141-145 |
NN |
denotes |
salt |
T7311 |
154-156 |
, |
denotes |
, |
T7312 |
156-165 |
VBN |
denotes |
collected |
T7313 |
166-168 |
IN |
denotes |
in |
T7314 |
169-172 |
NN |
denotes |
ice |
T7315 |
173-177 |
JJ |
denotes |
cold |
T7316 |
184-190 |
NN |
denotes |
buffer |
T7317 |
178-183 |
NN |
denotes |
lysis |
T7318 |
191-192 |
-LRB- |
denotes |
( |
T7319 |
198-203 |
NN |
denotes |
Hepes |
T7320 |
192-194 |
CD |
denotes |
20 |
T7321 |
195-197 |
NN |
denotes |
mM |
T7322 |
203-205 |
, |
denotes |
, |
T7323 |
205-207 |
NN |
denotes |
pH |
T7324 |
208-211 |
CD |
denotes |
7.4 |
T7325 |
211-213 |
, |
denotes |
, |
T7326 |
213-216 |
CD |
denotes |
200 |
T7327 |
217-219 |
NN |
denotes |
μM |
T7328 |
220-225 |
NN |
denotes |
CaCl2 |
T7329 |
225-227 |
, |
denotes |
, |
T7330 |
227-231 |
CD |
denotes |
0.25 |
T7331 |
232-233 |
NN |
denotes |
M |
T7332 |
234-241 |
NN |
denotes |
sucrose |
T7333 |
241-243 |
, |
denotes |
, |
T7334 |
243-246 |
CC |
denotes |
and |
T7335 |
247-257 |
NN |
denotes |
proteinase |
T7336 |
258-268 |
NNS |
denotes |
inhibitors |
T7337 |
269-270 |
-LRB- |
denotes |
( |
T7338 |
270-275 |
NNP |
denotes |
Roche |
T7339 |
275-276 |
-RRB- |
denotes |
) |
T7340 |
276-277 |
-RRB- |
denotes |
) |
T7341 |
277-279 |
, |
denotes |
, |
T7342 |
279-290 |
VBN |
denotes |
homogenized |
T7343 |
291-293 |
IN |
denotes |
by |
T7344 |
294-295 |
DT |
denotes |
a |
T7345 |
319-330 |
NN |
denotes |
homogenizer |
T7346 |
296-303 |
RB |
denotes |
loosely |
T7347 |
304-311 |
JJ |
denotes |
fitting |
T7348 |
312-318 |
NN |
denotes |
dounce |
T7349 |
331-334 |
CC |
denotes |
and |
T7350 |
335-345 |
JJ |
denotes |
additional |
T7351 |
346-354 |
NNS |
denotes |
passages |
T7352 |
355-362 |
IN |
denotes |
through |
T7353 |
363-364 |
DT |
denotes |
a |
T7354 |
374-380 |
NN |
denotes |
needle |
T7355 |
365-367 |
CD |
denotes |
22 |
T7356 |
368-373 |
NN |
denotes |
gauge |
T7357 |
380-381 |
. |
denotes |
. |
T7358 |
381-541 |
sentence |
denotes |
A 50 % iodixanol solution was established by mixing Optiprep solution (60 % iodixanol; Sigma) with buffer (120 mM Hepes, pH 7.4, 1.2 mM CaCl2, 0.25 M sucrose). |
T7359 |
382-383 |
DT |
denotes |
A |
T7360 |
399-407 |
NN |
denotes |
solution |
T7361 |
384-386 |
CD |
denotes |
50 |
T7362 |
387-388 |
NN |
denotes |
% |
T7363 |
389-398 |
NN |
denotes |
iodixanol |
T7364 |
412-423 |
VBN |
denotes |
established |
T7365 |
408-411 |
VBD |
denotes |
was |
T7366 |
424-426 |
IN |
denotes |
by |
T7367 |
427-433 |
VBG |
denotes |
mixing |
T7368 |
434-442 |
NN |
denotes |
Optiprep |
T7369 |
443-451 |
NN |
denotes |
solution |
T7370 |
452-453 |
-LRB- |
denotes |
( |
T7371 |
469-474 |
NN |
denotes |
Sigma |
T7372 |
453-455 |
CD |
denotes |
60 |
T7373 |
456-457 |
NN |
denotes |
% |
T7374 |
458-467 |
NN |
denotes |
iodixanol |
T7375 |
467-468 |
: |
denotes |
; |
T7376 |
474-475 |
-RRB- |
denotes |
) |
T7377 |
476-480 |
IN |
denotes |
with |
T7378 |
481-487 |
NN |
denotes |
buffer |
T7379 |
488-489 |
-LRB- |
denotes |
( |
T7380 |
532-539 |
NN |
denotes |
sucrose |
T7381 |
489-492 |
CD |
denotes |
120 |
T7382 |
493-495 |
NN |
denotes |
mM |
T7383 |
496-501 |
NN |
denotes |
Hepes |
T7384 |
501-503 |
, |
denotes |
, |
T7385 |
503-505 |
NN |
denotes |
pH |
T7386 |
506-509 |
CD |
denotes |
7.4 |
T7387 |
509-511 |
, |
denotes |
, |
T7388 |
511-514 |
CD |
denotes |
1.2 |
T7389 |
515-517 |
NN |
denotes |
mM |
T7390 |
518-523 |
NN |
denotes |
CaCl2 |
T7391 |
523-525 |
, |
denotes |
, |
T7392 |
525-529 |
CD |
denotes |
0.25 |
T7393 |
530-531 |
NN |
denotes |
M |
T7394 |
539-540 |
-RRB- |
denotes |
) |
T7395 |
540-541 |
. |
denotes |
. |
T7396 |
541-708 |
sentence |
denotes |
Seven parts of this 50 % iodixanol solution were mixed with thirteen parts of the red blood cell solution, generating a gradient solution containing 17.5 % iodixanol. |
T7397 |
542-547 |
CD |
denotes |
Seven |
T7398 |
548-553 |
NNS |
denotes |
parts |
T7399 |
591-596 |
VBN |
denotes |
mixed |
T7400 |
554-556 |
IN |
denotes |
of |
T7401 |
557-561 |
DT |
denotes |
this |
T7402 |
577-585 |
NN |
denotes |
solution |
T7403 |
562-564 |
CD |
denotes |
50 |
T7404 |
565-566 |
NN |
denotes |
% |
T7405 |
567-576 |
NN |
denotes |
iodixanol |
T7406 |
586-590 |
VBD |
denotes |
were |
T7407 |
597-601 |
IN |
denotes |
with |
T7408 |
602-610 |
CD |
denotes |
thirteen |
T7409 |
611-616 |
NNS |
denotes |
parts |
T7410 |
617-619 |
IN |
denotes |
of |
T7411 |
620-623 |
DT |
denotes |
the |
T7412 |
639-647 |
NN |
denotes |
solution |
T7413 |
624-627 |
JJ |
denotes |
red |
T7414 |
634-638 |
NN |
denotes |
cell |
T7415 |
628-633 |
NN |
denotes |
blood |
T7416 |
647-649 |
, |
denotes |
, |
T7417 |
649-659 |
VBG |
denotes |
generating |
T7418 |
660-661 |
DT |
denotes |
a |
T7419 |
671-679 |
NN |
denotes |
solution |
T7420 |
662-670 |
NN |
denotes |
gradient |
T7421 |
680-690 |
VBG |
denotes |
containing |
T7422 |
691-695 |
CD |
denotes |
17.5 |
T7423 |
696-697 |
NN |
denotes |
% |
T7424 |
698-707 |
NN |
denotes |
iodixanol |
T7425 |
707-708 |
. |
denotes |
. |
T7426 |
708-975 |
sentence |
denotes |
The mixture was filled into a centrifuge tube underlayed by cushions of 30 % and 35 % iodixanol and centrifuged for 3 hours at 270,000 × g in a SW41 Ti swing out rotor (Beckman) to achieve a nearly linear density gradient (approximately 1.06 g/ml to 1.20 g/ml) [24]. |
T7427 |
709-712 |
DT |
denotes |
The |
T7428 |
713-720 |
NN |
denotes |
mixture |
T7429 |
725-731 |
VBN |
denotes |
filled |
T7430 |
721-724 |
VBD |
denotes |
was |
T7431 |
732-736 |
IN |
denotes |
into |
T7432 |
737-738 |
DT |
denotes |
a |
T7433 |
750-754 |
NN |
denotes |
tube |
T7434 |
739-749 |
JJ |
denotes |
centrifuge |
T7435 |
755-765 |
VBN |
denotes |
underlayed |
T7436 |
766-768 |
IN |
denotes |
by |
T7437 |
769-777 |
NNS |
denotes |
cushions |
T7438 |
778-780 |
IN |
denotes |
of |
T7439 |
781-783 |
CD |
denotes |
30 |
T7440 |
784-785 |
NN |
denotes |
% |
T7441 |
795-804 |
NN |
denotes |
iodixanol |
T7442 |
786-789 |
CC |
denotes |
and |
T7443 |
790-792 |
CD |
denotes |
35 |
T7444 |
793-794 |
NN |
denotes |
% |
T7445 |
805-808 |
CC |
denotes |
and |
T7446 |
809-820 |
VBN |
denotes |
centrifuged |
T7447 |
821-824 |
IN |
denotes |
for |
T7448 |
825-826 |
CD |
denotes |
3 |
T7449 |
827-832 |
NNS |
denotes |
hours |
T7450 |
833-835 |
IN |
denotes |
at |
T7451 |
836-843 |
CD |
denotes |
270,000 |
T7452 |
846-847 |
NN |
denotes |
g |
T7453 |
844-845 |
SYM |
denotes |
× |
T7454 |
848-850 |
IN |
denotes |
in |
T7455 |
851-852 |
DT |
denotes |
a |
T7456 |
871-876 |
NN |
denotes |
rotor |
T7457 |
853-857 |
NN |
denotes |
SW41 |
T7458 |
858-860 |
NNP |
denotes |
Ti |
T7459 |
861-866 |
NN |
denotes |
swing |
T7460 |
867-870 |
NN |
denotes |
out |
T7461 |
877-878 |
-LRB- |
denotes |
( |
T7462 |
878-885 |
NN |
denotes |
Beckman |
T7463 |
885-886 |
-RRB- |
denotes |
) |
T7464 |
887-889 |
TO |
denotes |
to |
T7465 |
890-897 |
VB |
denotes |
achieve |
T7466 |
898-899 |
DT |
denotes |
a |
T7467 |
922-930 |
NN |
denotes |
gradient |
T7468 |
900-906 |
RB |
denotes |
nearly |
T7469 |
907-913 |
JJ |
denotes |
linear |
T7470 |
914-921 |
NN |
denotes |
density |
T7471 |
931-932 |
-LRB- |
denotes |
( |
T7472 |
951-952 |
NNS |
denotes |
g |
T7473 |
932-945 |
RB |
denotes |
approximately |
T7474 |
946-950 |
CD |
denotes |
1.06 |
T7475 |
952-953 |
SYM |
denotes |
/ |
T7476 |
953-955 |
NN |
denotes |
ml |
T7477 |
956-958 |
IN |
denotes |
to |
T7478 |
959-963 |
CD |
denotes |
1.20 |
T7479 |
964-965 |
NNS |
denotes |
g |
T7480 |
965-966 |
SYM |
denotes |
/ |
T7481 |
966-968 |
NN |
denotes |
ml |
T7482 |
968-969 |
-RRB- |
denotes |
) |
T7483 |
970-971 |
-LRB- |
denotes |
[ |
T7484 |
971-973 |
CD |
denotes |
24 |
T7485 |
973-974 |
-RRB- |
denotes |
] |
T7486 |
974-975 |
. |
denotes |
. |
T7487 |
975-1139 |
sentence |
denotes |
Fractions of 0.6 ml were collected from the top of the gradient by a 1 ml syringe without using a needle and immediately frozen in liquid nitrogen for further use. |
T7488 |
976-985 |
NNS |
denotes |
Fractions |
T7489 |
1001-1010 |
VBN |
denotes |
collected |
T7490 |
986-988 |
IN |
denotes |
of |
T7491 |
989-992 |
CD |
denotes |
0.6 |
T7492 |
993-995 |
NN |
denotes |
ml |
T7493 |
996-1000 |
VBD |
denotes |
were |
T7494 |
1011-1015 |
IN |
denotes |
from |
T7495 |
1016-1019 |
DT |
denotes |
the |
T7496 |
1020-1023 |
NN |
denotes |
top |
T7497 |
1024-1026 |
IN |
denotes |
of |
T7498 |
1027-1030 |
DT |
denotes |
the |
T7499 |
1031-1039 |
NN |
denotes |
gradient |
T7500 |
1040-1042 |
IN |
denotes |
by |
T7501 |
1043-1044 |
DT |
denotes |
a |
T7502 |
1050-1057 |
NN |
denotes |
syringe |
T7503 |
1045-1046 |
CD |
denotes |
1 |
T7504 |
1047-1049 |
NN |
denotes |
ml |
T7505 |
1058-1065 |
IN |
denotes |
without |
T7506 |
1066-1071 |
VBG |
denotes |
using |
T7507 |
1072-1073 |
DT |
denotes |
a |
T7508 |
1074-1080 |
NN |
denotes |
needle |
T7509 |
1081-1084 |
CC |
denotes |
and |
T7510 |
1085-1096 |
RB |
denotes |
immediately |
T7511 |
1097-1103 |
VBN |
denotes |
frozen |
T7512 |
1104-1106 |
IN |
denotes |
in |
T7513 |
1107-1113 |
JJ |
denotes |
liquid |
T7514 |
1114-1122 |
NN |
denotes |
nitrogen |
T7515 |
1123-1126 |
IN |
denotes |
for |
T7516 |
1127-1134 |
JJ |
denotes |
further |
T7517 |
1135-1138 |
NN |
denotes |
use |
T7518 |
1138-1139 |
. |
denotes |
. |
R4440 |
T7284 |
T7285 |
det |
The,distribution |
R4441 |
T7285 |
T7287 |
nsubjpass |
distribution,addressed |
R4442 |
T7286 |
T7285 |
amod |
subcellular,distribution |
R4443 |
T7288 |
T7285 |
prep |
of,distribution |
R4444 |
T7289 |
T7290 |
compound |
annexin,A7 |
R4445 |
T7290 |
T7288 |
pobj |
A7,of |
R4446 |
T7291 |
T7287 |
auxpass |
was,addressed |
R4447 |
T7292 |
T7287 |
prep |
according,addressed |
R4448 |
T7293 |
T7292 |
prep |
to,according |
R4449 |
T7294 |
T7293 |
punct |
[,to |
R4450 |
T7295 |
T7293 |
pobj |
24,to |
R4451 |
T7296 |
T7287 |
punct |
],addressed |
R4452 |
T7297 |
T7287 |
punct |
.,addressed |
R4453 |
T7299 |
T7300 |
amod |
Red,cells |
R4454 |
T7300 |
T7302 |
nsubjpass |
cells,centrifuged |
R4455 |
T7301 |
T7300 |
compound |
blood,cells |
R4456 |
T7303 |
T7302 |
auxpass |
were,centrifuged |
R4457 |
T7304 |
T7302 |
cc |
and,centrifuged |
R4458 |
T7305 |
T7302 |
conj |
washed,centrifuged |
R4459 |
T7306 |
T7305 |
prep |
in,washed |
R4460 |
T7307 |
T7308 |
amod |
isotonic,solution |
R4461 |
T7308 |
T7306 |
pobj |
solution,in |
R4462 |
T7309 |
T7310 |
amod |
buffered,salt |
R4463 |
T7310 |
T7308 |
compound |
salt,solution |
R4464 |
T7311 |
T7305 |
punct |
", ",washed |
R4465 |
T7312 |
T7305 |
conj |
collected,washed |
R4466 |
T7313 |
T7312 |
prep |
in,collected |
R4467 |
T7314 |
T7315 |
npadvmod |
ice,cold |
R4468 |
T7315 |
T7316 |
amod |
cold,buffer |
R4469 |
T7316 |
T7313 |
pobj |
buffer,in |
R4470 |
T7317 |
T7316 |
compound |
lysis,buffer |
R4471 |
T7318 |
T7319 |
punct |
(,Hepes |
R4472 |
T7319 |
T7316 |
parataxis |
Hepes,buffer |
R4473 |
T7320 |
T7321 |
nummod |
20,mM |
R4474 |
T7321 |
T7319 |
compound |
mM,Hepes |
R4475 |
T7322 |
T7319 |
punct |
", ",Hepes |
R4476 |
T7323 |
T7319 |
npadvmod |
pH,Hepes |
R4477 |
T7324 |
T7323 |
nummod |
7.4,pH |
R4478 |
T7325 |
T7319 |
punct |
", ",Hepes |
R4479 |
T7326 |
T7327 |
nummod |
200,μM |
R4480 |
T7327 |
T7328 |
compound |
μM,CaCl2 |
R4481 |
T7328 |
T7319 |
conj |
CaCl2,Hepes |
R4482 |
T7329 |
T7328 |
punct |
", ",CaCl2 |
R4483 |
T7330 |
T7331 |
nummod |
0.25,M |
R4484 |
T7331 |
T7332 |
compound |
M,sucrose |
R4485 |
T7332 |
T7328 |
conj |
sucrose,CaCl2 |
R4486 |
T7333 |
T7332 |
punct |
", ",sucrose |
R4487 |
T7334 |
T7332 |
cc |
and,sucrose |
R4488 |
T7335 |
T7336 |
compound |
proteinase,inhibitors |
R4489 |
T7336 |
T7332 |
conj |
inhibitors,sucrose |
R4490 |
T7337 |
T7338 |
punct |
(,Roche |
R4491 |
T7338 |
T7336 |
parataxis |
Roche,inhibitors |
R4492 |
T7339 |
T7338 |
punct |
),Roche |
R4493 |
T7340 |
T7336 |
punct |
),inhibitors |
R4494 |
T7341 |
T7312 |
punct |
", ",collected |
R4495 |
T7342 |
T7312 |
conj |
homogenized,collected |
R4496 |
T7343 |
T7342 |
prep |
by,homogenized |
R4497 |
T7344 |
T7345 |
det |
a,homogenizer |
R4498 |
T7345 |
T7343 |
pobj |
homogenizer,by |
R4499 |
T7346 |
T7347 |
advmod |
loosely,fitting |
R4500 |
T7347 |
T7345 |
amod |
fitting,homogenizer |
R4501 |
T7348 |
T7345 |
compound |
dounce,homogenizer |
R4502 |
T7349 |
T7345 |
cc |
and,homogenizer |
R4503 |
T7350 |
T7351 |
amod |
additional,passages |
R4504 |
T7351 |
T7345 |
conj |
passages,homogenizer |
R4505 |
T7352 |
T7351 |
prep |
through,passages |
R4506 |
T7353 |
T7354 |
det |
a,needle |
R4507 |
T7354 |
T7352 |
pobj |
needle,through |
R4508 |
T7355 |
T7356 |
nummod |
22,gauge |
R4509 |
T7356 |
T7354 |
compound |
gauge,needle |
R4510 |
T7357 |
T7302 |
punct |
.,centrifuged |
R4511 |
T7359 |
T7360 |
det |
A,solution |
R4512 |
T7360 |
T7364 |
nsubjpass |
solution,established |
R4513 |
T7361 |
T7362 |
nummod |
50,% |
R4514 |
T7362 |
T7360 |
compound |
%,solution |
R4515 |
T7363 |
T7360 |
compound |
iodixanol,solution |
R4516 |
T7365 |
T7364 |
auxpass |
was,established |
R4517 |
T7366 |
T7364 |
prep |
by,established |
R4518 |
T7367 |
T7366 |
pcomp |
mixing,by |
R4519 |
T7368 |
T7369 |
compound |
Optiprep,solution |
R4520 |
T7369 |
T7367 |
dobj |
solution,mixing |
R4521 |
T7370 |
T7371 |
punct |
(,Sigma |
R4522 |
T7371 |
T7369 |
parataxis |
Sigma,solution |
R4523 |
T7372 |
T7373 |
nummod |
60,% |
R4524 |
T7373 |
T7374 |
compound |
%,iodixanol |
R4525 |
T7374 |
T7371 |
dep |
iodixanol,Sigma |
R4526 |
T7375 |
T7371 |
punct |
;,Sigma |
R4527 |
T7376 |
T7371 |
punct |
),Sigma |
R4528 |
T7377 |
T7367 |
prep |
with,mixing |
R4529 |
T7378 |
T7377 |
pobj |
buffer,with |
R4530 |
T7379 |
T7380 |
punct |
(,sucrose |
R4531 |
T7380 |
T7378 |
parataxis |
sucrose,buffer |
R4532 |
T7381 |
T7382 |
nummod |
120,mM |
R4533 |
T7382 |
T7383 |
compound |
mM,Hepes |
R4534 |
T7383 |
T7380 |
dep |
Hepes,sucrose |
R4535 |
T7384 |
T7383 |
punct |
", ",Hepes |
R4536 |
T7385 |
T7383 |
npadvmod |
pH,Hepes |
R4537 |
T7386 |
T7385 |
nummod |
7.4,pH |
R4538 |
T7387 |
T7380 |
punct |
", ",sucrose |
R4539 |
T7388 |
T7389 |
nummod |
1.2,mM |
R4540 |
T7389 |
T7390 |
compound |
mM,CaCl2 |
R4541 |
T7390 |
T7380 |
dep |
CaCl2,sucrose |
R4542 |
T7391 |
T7380 |
punct |
", ",sucrose |
R4543 |
T7392 |
T7393 |
nummod |
0.25,M |
R4544 |
T7393 |
T7380 |
compound |
M,sucrose |
R4545 |
T7394 |
T7380 |
punct |
),sucrose |
R4546 |
T7395 |
T7364 |
punct |
.,established |
R4547 |
T7397 |
T7398 |
nummod |
Seven,parts |
R4548 |
T7398 |
T7399 |
nsubjpass |
parts,mixed |
R4549 |
T7400 |
T7398 |
prep |
of,parts |
R4550 |
T7401 |
T7402 |
det |
this,solution |
R4551 |
T7402 |
T7400 |
pobj |
solution,of |
R4552 |
T7403 |
T7404 |
nummod |
50,% |
R4553 |
T7404 |
T7402 |
compound |
%,solution |
R4554 |
T7405 |
T7402 |
compound |
iodixanol,solution |
R4555 |
T7406 |
T7399 |
auxpass |
were,mixed |
R4556 |
T7407 |
T7399 |
prep |
with,mixed |
R4557 |
T7408 |
T7409 |
nummod |
thirteen,parts |
R4558 |
T7409 |
T7407 |
pobj |
parts,with |
R4559 |
T7410 |
T7409 |
prep |
of,parts |
R4560 |
T7411 |
T7412 |
det |
the,solution |
R4561 |
T7412 |
T7410 |
pobj |
solution,of |
R4562 |
T7413 |
T7414 |
amod |
red,cell |
R4563 |
T7414 |
T7412 |
compound |
cell,solution |
R4564 |
T7415 |
T7414 |
compound |
blood,cell |
R4565 |
T7416 |
T7399 |
punct |
", ",mixed |
R4566 |
T7417 |
T7399 |
advcl |
generating,mixed |
R4567 |
T7418 |
T7419 |
det |
a,solution |
R4568 |
T7419 |
T7417 |
dobj |
solution,generating |
R4569 |
T7420 |
T7419 |
compound |
gradient,solution |
R4570 |
T7421 |
T7419 |
acl |
containing,solution |
R4571 |
T7422 |
T7423 |
nummod |
17.5,% |
R4572 |
T7423 |
T7424 |
compound |
%,iodixanol |
R4573 |
T7424 |
T7421 |
dobj |
iodixanol,containing |
R4574 |
T7425 |
T7399 |
punct |
.,mixed |
R4575 |
T7427 |
T7428 |
det |
The,mixture |
R4576 |
T7428 |
T7429 |
nsubj |
mixture,filled |
R4577 |
T7430 |
T7429 |
aux |
was,filled |
R4578 |
T7431 |
T7429 |
prep |
into,filled |
R4579 |
T7432 |
T7433 |
det |
a,tube |
R4580 |
T7433 |
T7431 |
pobj |
tube,into |
R4581 |
T7434 |
T7433 |
amod |
centrifuge,tube |
R4582 |
T7435 |
T7433 |
amod |
underlayed,tube |
R4583 |
T7436 |
T7435 |
prep |
by,underlayed |
R4584 |
T7437 |
T7436 |
pobj |
cushions,by |
R4585 |
T7438 |
T7437 |
prep |
of,cushions |
R4586 |
T7439 |
T7440 |
nummod |
30,% |
R4587 |
T7440 |
T7441 |
nmod |
%,iodixanol |
R4588 |
T7441 |
T7438 |
pobj |
iodixanol,of |
R4589 |
T7442 |
T7440 |
cc |
and,% |
R4590 |
T7443 |
T7444 |
nummod |
35,% |
R4591 |
T7444 |
T7440 |
conj |
%,% |
R4592 |
T7445 |
T7429 |
cc |
and,filled |
R4593 |
T7446 |
T7429 |
conj |
centrifuged,filled |
R4594 |
T7447 |
T7446 |
prep |
for,centrifuged |
R4595 |
T7448 |
T7449 |
nummod |
3,hours |
R4596 |
T7449 |
T7447 |
pobj |
hours,for |
R4597 |
T7450 |
T7446 |
prep |
at,centrifuged |
R4598 |
T7451 |
T7452 |
nummod |
"270,000",g |
R4599 |
T7452 |
T7450 |
pobj |
g,at |
R4600 |
T7453 |
T7452 |
punct |
×,g |
R4601 |
T7454 |
T7446 |
prep |
in,centrifuged |
R4602 |
T7455 |
T7456 |
det |
a,rotor |
R4603 |
T7456 |
T7454 |
pobj |
rotor,in |
R4604 |
T7457 |
T7458 |
compound |
SW41,Ti |
R4605 |
T7458 |
T7456 |
compound |
Ti,rotor |
R4606 |
T7459 |
T7460 |
compound |
swing,out |
R4607 |
T7460 |
T7456 |
compound |
out,rotor |
R4608 |
T7461 |
T7462 |
punct |
(,Beckman |
R4609 |
T7462 |
T7456 |
parataxis |
Beckman,rotor |
R4610 |
T7463 |
T7462 |
punct |
),Beckman |
R4611 |
T7464 |
T7465 |
aux |
to,achieve |
R4612 |
T7465 |
T7429 |
advcl |
achieve,filled |
R4613 |
T7466 |
T7467 |
det |
a,gradient |
R4614 |
T7467 |
T7465 |
dobj |
gradient,achieve |
R4615 |
T7468 |
T7469 |
advmod |
nearly,linear |
R4616 |
T7469 |
T7467 |
amod |
linear,gradient |
R4617 |
T7470 |
T7467 |
compound |
density,gradient |
R4618 |
T7471 |
T7472 |
punct |
(,g |
R4619 |
T7472 |
T7467 |
parataxis |
g,gradient |
R4620 |
T7473 |
T7472 |
advmod |
approximately,g |
R4621 |
T7474 |
T7472 |
nummod |
1.06,g |
R4622 |
T7475 |
T7476 |
punct |
/,ml |
R4623 |
T7476 |
T7472 |
prep |
ml,g |
R4624 |
T7477 |
T7472 |
prep |
to,g |
R4625 |
T7478 |
T7479 |
nummod |
1.20,g |
R4626 |
T7479 |
T7477 |
pobj |
g,to |
R4627 |
T7480 |
T7481 |
punct |
/,ml |
R4628 |
T7481 |
T7479 |
prep |
ml,g |
R4629 |
T7482 |
T7472 |
punct |
),g |
R4630 |
T7483 |
T7484 |
punct |
[,24 |
R4631 |
T7484 |
T7429 |
parataxis |
24,filled |
R4632 |
T7485 |
T7484 |
punct |
],24 |
R4633 |
T7486 |
T7429 |
punct |
.,filled |
R4634 |
T7488 |
T7489 |
nsubjpass |
Fractions,collected |
R4635 |
T7490 |
T7488 |
prep |
of,Fractions |
R4636 |
T7491 |
T7492 |
nummod |
0.6,ml |
R4637 |
T7492 |
T7490 |
pobj |
ml,of |
R4638 |
T7493 |
T7489 |
auxpass |
were,collected |
R4639 |
T7494 |
T7489 |
prep |
from,collected |
R4640 |
T7495 |
T7496 |
det |
the,top |
R4641 |
T7496 |
T7494 |
pobj |
top,from |
R4642 |
T7497 |
T7496 |
prep |
of,top |
R4643 |
T7498 |
T7499 |
det |
the,gradient |
R4644 |
T7499 |
T7497 |
pobj |
gradient,of |
R4645 |
T7500 |
T7489 |
prep |
by,collected |
R4646 |
T7501 |
T7502 |
det |
a,syringe |
R4647 |
T7502 |
T7500 |
pobj |
syringe,by |
R4648 |
T7503 |
T7504 |
nummod |
1,ml |
R4649 |
T7504 |
T7502 |
compound |
ml,syringe |
R4650 |
T7505 |
T7489 |
prep |
without,collected |
R4651 |
T7506 |
T7505 |
pcomp |
using,without |
R4652 |
T7507 |
T7508 |
det |
a,needle |
R4653 |
T7508 |
T7506 |
dobj |
needle,using |
R4654 |
T7509 |
T7489 |
cc |
and,collected |
R4655 |
T7510 |
T7511 |
advmod |
immediately,frozen |
R4656 |
T7511 |
T7489 |
conj |
frozen,collected |
R4657 |
T7512 |
T7511 |
prep |
in,frozen |
R4658 |
T7513 |
T7514 |
amod |
liquid,nitrogen |
R4659 |
T7514 |
T7512 |
pobj |
nitrogen,in |
R4660 |
T7515 |
T7511 |
prep |
for,frozen |
R4661 |
T7516 |
T7517 |
amod |
further,use |
R4662 |
T7517 |
T7515 |
pobj |
use,for |
R4663 |
T7518 |
T7489 |
punct |
.,collected |