Id |
Subject |
Object |
Predicate |
Lexical cue |
T7273 |
0-4 |
NN |
denotes |
Self |
T7274 |
5-12 |
VBG |
denotes |
forming |
T7275 |
31-40 |
NNS |
denotes |
gradients |
T7276 |
13-22 |
NN |
denotes |
iodixanol |
T7277 |
23-30 |
NN |
denotes |
density |
T7278 |
41-43 |
IN |
denotes |
of |
T7279 |
44-49 |
JJ |
denotes |
human |
T7280 |
60-65 |
NNS |
denotes |
cells |
T7281 |
50-53 |
JJ |
denotes |
red |
T7282 |
54-59 |
NN |
denotes |
blood |
T7283 |
65-141 |
sentence |
denotes |
The subcellular distribution of annexin A7 was addressed according to [24]. |
T7284 |
66-69 |
DT |
denotes |
The |
T7285 |
82-94 |
NN |
denotes |
distribution |
T7286 |
70-81 |
JJ |
denotes |
subcellular |
T7287 |
113-122 |
VBN |
denotes |
addressed |
T7288 |
95-97 |
IN |
denotes |
of |
T7289 |
98-105 |
NN |
denotes |
annexin |
T7290 |
106-108 |
NN |
denotes |
A7 |
T7291 |
109-112 |
VBD |
denotes |
was |
T7292 |
123-132 |
VBG |
denotes |
according |
T7293 |
133-135 |
IN |
denotes |
to |
T7294 |
136-137 |
-LRB- |
denotes |
[ |
T7295 |
137-139 |
CD |
denotes |
24 |
T7296 |
139-140 |
-RRB- |
denotes |
] |
T7297 |
140-141 |
. |
denotes |
. |
T7298 |
141-447 |
sentence |
denotes |
Red blood cells were centrifuged and washed in isotonic buffered salt solution, collected in ice cold lysis buffer (20 mM Hepes, pH 7.4, 200 μM CaCl2, 0.25 M sucrose, and proteinase inhibitors (Roche)), homogenized by a loosely fitting dounce homogenizer and additional passages through a 22 gauge needle. |
T7299 |
142-145 |
JJ |
denotes |
Red |
T7300 |
152-157 |
NNS |
denotes |
cells |
T7301 |
146-151 |
NN |
denotes |
blood |
T7302 |
163-174 |
VBN |
denotes |
centrifuged |
T7303 |
158-162 |
VBD |
denotes |
were |
T7304 |
175-178 |
CC |
denotes |
and |
T7305 |
179-185 |
VBN |
denotes |
washed |
T7306 |
186-188 |
IN |
denotes |
in |
T7307 |
189-197 |
JJ |
denotes |
isotonic |
T7308 |
212-220 |
NN |
denotes |
solution |
T7309 |
198-206 |
VBN |
denotes |
buffered |
T7310 |
207-211 |
NN |
denotes |
salt |
T7311 |
220-222 |
, |
denotes |
, |
T7312 |
222-231 |
VBN |
denotes |
collected |
T7313 |
232-234 |
IN |
denotes |
in |
T7314 |
235-238 |
NN |
denotes |
ice |
T7315 |
239-243 |
JJ |
denotes |
cold |
T7316 |
250-256 |
NN |
denotes |
buffer |
T7317 |
244-249 |
NN |
denotes |
lysis |
T7318 |
257-258 |
-LRB- |
denotes |
( |
T7319 |
264-269 |
NN |
denotes |
Hepes |
T7320 |
258-260 |
CD |
denotes |
20 |
T7321 |
261-263 |
NN |
denotes |
mM |
T7322 |
269-271 |
, |
denotes |
, |
T7323 |
271-273 |
NN |
denotes |
pH |
T7324 |
274-277 |
CD |
denotes |
7.4 |
T7325 |
277-279 |
, |
denotes |
, |
T7326 |
279-282 |
CD |
denotes |
200 |
T7327 |
283-285 |
NN |
denotes |
μM |
T7328 |
286-291 |
NN |
denotes |
CaCl2 |
T7329 |
291-293 |
, |
denotes |
, |
T7330 |
293-297 |
CD |
denotes |
0.25 |
T7331 |
298-299 |
NN |
denotes |
M |
T7332 |
300-307 |
NN |
denotes |
sucrose |
T7333 |
307-309 |
, |
denotes |
, |
T7334 |
309-312 |
CC |
denotes |
and |
T7335 |
313-323 |
NN |
denotes |
proteinase |
T7336 |
324-334 |
NNS |
denotes |
inhibitors |
T7337 |
335-336 |
-LRB- |
denotes |
( |
T7338 |
336-341 |
NNP |
denotes |
Roche |
T7339 |
341-342 |
-RRB- |
denotes |
) |
T7340 |
342-343 |
-RRB- |
denotes |
) |
T7341 |
343-345 |
, |
denotes |
, |
T7342 |
345-356 |
VBN |
denotes |
homogenized |
T7343 |
357-359 |
IN |
denotes |
by |
T7344 |
360-361 |
DT |
denotes |
a |
T7345 |
385-396 |
NN |
denotes |
homogenizer |
T7346 |
362-369 |
RB |
denotes |
loosely |
T7347 |
370-377 |
JJ |
denotes |
fitting |
T7348 |
378-384 |
NN |
denotes |
dounce |
T7349 |
397-400 |
CC |
denotes |
and |
T7350 |
401-411 |
JJ |
denotes |
additional |
T7351 |
412-420 |
NNS |
denotes |
passages |
T7352 |
421-428 |
IN |
denotes |
through |
T7353 |
429-430 |
DT |
denotes |
a |
T7354 |
440-446 |
NN |
denotes |
needle |
T7355 |
431-433 |
CD |
denotes |
22 |
T7356 |
434-439 |
NN |
denotes |
gauge |
T7357 |
446-447 |
. |
denotes |
. |
T7358 |
447-607 |
sentence |
denotes |
A 50 % iodixanol solution was established by mixing Optiprep solution (60 % iodixanol; Sigma) with buffer (120 mM Hepes, pH 7.4, 1.2 mM CaCl2, 0.25 M sucrose). |
T7359 |
448-449 |
DT |
denotes |
A |
T7360 |
465-473 |
NN |
denotes |
solution |
T7361 |
450-452 |
CD |
denotes |
50 |
T7362 |
453-454 |
NN |
denotes |
% |
T7363 |
455-464 |
NN |
denotes |
iodixanol |
T7364 |
478-489 |
VBN |
denotes |
established |
T7365 |
474-477 |
VBD |
denotes |
was |
T7366 |
490-492 |
IN |
denotes |
by |
T7367 |
493-499 |
VBG |
denotes |
mixing |
T7368 |
500-508 |
NN |
denotes |
Optiprep |
T7369 |
509-517 |
NN |
denotes |
solution |
T7370 |
518-519 |
-LRB- |
denotes |
( |
T7371 |
535-540 |
NN |
denotes |
Sigma |
T7372 |
519-521 |
CD |
denotes |
60 |
T7373 |
522-523 |
NN |
denotes |
% |
T7374 |
524-533 |
NN |
denotes |
iodixanol |
T7375 |
533-534 |
: |
denotes |
; |
T7376 |
540-541 |
-RRB- |
denotes |
) |
T7377 |
542-546 |
IN |
denotes |
with |
T7378 |
547-553 |
NN |
denotes |
buffer |
T7379 |
554-555 |
-LRB- |
denotes |
( |
T7380 |
598-605 |
NN |
denotes |
sucrose |
T7381 |
555-558 |
CD |
denotes |
120 |
T7382 |
559-561 |
NN |
denotes |
mM |
T7383 |
562-567 |
NN |
denotes |
Hepes |
T7384 |
567-569 |
, |
denotes |
, |
T7385 |
569-571 |
NN |
denotes |
pH |
T7386 |
572-575 |
CD |
denotes |
7.4 |
T7387 |
575-577 |
, |
denotes |
, |
T7388 |
577-580 |
CD |
denotes |
1.2 |
T7389 |
581-583 |
NN |
denotes |
mM |
T7390 |
584-589 |
NN |
denotes |
CaCl2 |
T7391 |
589-591 |
, |
denotes |
, |
T7392 |
591-595 |
CD |
denotes |
0.25 |
T7393 |
596-597 |
NN |
denotes |
M |
T7394 |
605-606 |
-RRB- |
denotes |
) |
T7395 |
606-607 |
. |
denotes |
. |
T7396 |
607-774 |
sentence |
denotes |
Seven parts of this 50 % iodixanol solution were mixed with thirteen parts of the red blood cell solution, generating a gradient solution containing 17.5 % iodixanol. |
T7397 |
608-613 |
CD |
denotes |
Seven |
T7398 |
614-619 |
NNS |
denotes |
parts |
T7399 |
657-662 |
VBN |
denotes |
mixed |
T7400 |
620-622 |
IN |
denotes |
of |
T7401 |
623-627 |
DT |
denotes |
this |
T7402 |
643-651 |
NN |
denotes |
solution |
T7403 |
628-630 |
CD |
denotes |
50 |
T7404 |
631-632 |
NN |
denotes |
% |
T7405 |
633-642 |
NN |
denotes |
iodixanol |
T7406 |
652-656 |
VBD |
denotes |
were |
T7407 |
663-667 |
IN |
denotes |
with |
T7408 |
668-676 |
CD |
denotes |
thirteen |
T7409 |
677-682 |
NNS |
denotes |
parts |
T7410 |
683-685 |
IN |
denotes |
of |
T7411 |
686-689 |
DT |
denotes |
the |
T7412 |
705-713 |
NN |
denotes |
solution |
T7413 |
690-693 |
JJ |
denotes |
red |
T7414 |
700-704 |
NN |
denotes |
cell |
T7415 |
694-699 |
NN |
denotes |
blood |
T7416 |
713-715 |
, |
denotes |
, |
T7417 |
715-725 |
VBG |
denotes |
generating |
T7418 |
726-727 |
DT |
denotes |
a |
T7419 |
737-745 |
NN |
denotes |
solution |
T7420 |
728-736 |
NN |
denotes |
gradient |
T7421 |
746-756 |
VBG |
denotes |
containing |
T7422 |
757-761 |
CD |
denotes |
17.5 |
T7423 |
762-763 |
NN |
denotes |
% |
T7424 |
764-773 |
NN |
denotes |
iodixanol |
T7425 |
773-774 |
. |
denotes |
. |
T7426 |
774-1041 |
sentence |
denotes |
The mixture was filled into a centrifuge tube underlayed by cushions of 30 % and 35 % iodixanol and centrifuged for 3 hours at 270,000 × g in a SW41 Ti swing out rotor (Beckman) to achieve a nearly linear density gradient (approximately 1.06 g/ml to 1.20 g/ml) [24]. |
T7427 |
775-778 |
DT |
denotes |
The |
T7428 |
779-786 |
NN |
denotes |
mixture |
T7429 |
791-797 |
VBN |
denotes |
filled |
T7430 |
787-790 |
VBD |
denotes |
was |
T7431 |
798-802 |
IN |
denotes |
into |
T7432 |
803-804 |
DT |
denotes |
a |
T7433 |
816-820 |
NN |
denotes |
tube |
T7434 |
805-815 |
JJ |
denotes |
centrifuge |
T7435 |
821-831 |
VBN |
denotes |
underlayed |
T7436 |
832-834 |
IN |
denotes |
by |
T7437 |
835-843 |
NNS |
denotes |
cushions |
T7438 |
844-846 |
IN |
denotes |
of |
T7439 |
847-849 |
CD |
denotes |
30 |
T7440 |
850-851 |
NN |
denotes |
% |
T7441 |
861-870 |
NN |
denotes |
iodixanol |
T7442 |
852-855 |
CC |
denotes |
and |
T7443 |
856-858 |
CD |
denotes |
35 |
T7444 |
859-860 |
NN |
denotes |
% |
T7445 |
871-874 |
CC |
denotes |
and |
T7446 |
875-886 |
VBN |
denotes |
centrifuged |
T7447 |
887-890 |
IN |
denotes |
for |
T7448 |
891-892 |
CD |
denotes |
3 |
T7449 |
893-898 |
NNS |
denotes |
hours |
T7450 |
899-901 |
IN |
denotes |
at |
T7451 |
902-909 |
CD |
denotes |
270,000 |
T7452 |
912-913 |
NN |
denotes |
g |
T7453 |
910-911 |
SYM |
denotes |
× |
T7454 |
914-916 |
IN |
denotes |
in |
T7455 |
917-918 |
DT |
denotes |
a |
T7456 |
937-942 |
NN |
denotes |
rotor |
T7457 |
919-923 |
NN |
denotes |
SW41 |
T7458 |
924-926 |
NNP |
denotes |
Ti |
T7459 |
927-932 |
NN |
denotes |
swing |
T7460 |
933-936 |
NN |
denotes |
out |
T7461 |
943-944 |
-LRB- |
denotes |
( |
T7462 |
944-951 |
NN |
denotes |
Beckman |
T7463 |
951-952 |
-RRB- |
denotes |
) |
T7464 |
953-955 |
TO |
denotes |
to |
T7465 |
956-963 |
VB |
denotes |
achieve |
T7466 |
964-965 |
DT |
denotes |
a |
T7467 |
988-996 |
NN |
denotes |
gradient |
T7468 |
966-972 |
RB |
denotes |
nearly |
T7469 |
973-979 |
JJ |
denotes |
linear |
T7470 |
980-987 |
NN |
denotes |
density |
T7471 |
997-998 |
-LRB- |
denotes |
( |
T7472 |
1017-1018 |
NNS |
denotes |
g |
T7473 |
998-1011 |
RB |
denotes |
approximately |
T7474 |
1012-1016 |
CD |
denotes |
1.06 |
T7475 |
1018-1019 |
SYM |
denotes |
/ |
T7476 |
1019-1021 |
NN |
denotes |
ml |
T7477 |
1022-1024 |
IN |
denotes |
to |
T7478 |
1025-1029 |
CD |
denotes |
1.20 |
T7479 |
1030-1031 |
NNS |
denotes |
g |
T7480 |
1031-1032 |
SYM |
denotes |
/ |
T7481 |
1032-1034 |
NN |
denotes |
ml |
T7482 |
1034-1035 |
-RRB- |
denotes |
) |
T7483 |
1036-1037 |
-LRB- |
denotes |
[ |
T7484 |
1037-1039 |
CD |
denotes |
24 |
T7485 |
1039-1040 |
-RRB- |
denotes |
] |
T7486 |
1040-1041 |
. |
denotes |
. |
T7487 |
1041-1205 |
sentence |
denotes |
Fractions of 0.6 ml were collected from the top of the gradient by a 1 ml syringe without using a needle and immediately frozen in liquid nitrogen for further use. |
T7488 |
1042-1051 |
NNS |
denotes |
Fractions |
T7489 |
1067-1076 |
VBN |
denotes |
collected |
T7490 |
1052-1054 |
IN |
denotes |
of |
T7491 |
1055-1058 |
CD |
denotes |
0.6 |
T7492 |
1059-1061 |
NN |
denotes |
ml |
T7493 |
1062-1066 |
VBD |
denotes |
were |
T7494 |
1077-1081 |
IN |
denotes |
from |
T7495 |
1082-1085 |
DT |
denotes |
the |
T7496 |
1086-1089 |
NN |
denotes |
top |
T7497 |
1090-1092 |
IN |
denotes |
of |
T7498 |
1093-1096 |
DT |
denotes |
the |
T7499 |
1097-1105 |
NN |
denotes |
gradient |
T7500 |
1106-1108 |
IN |
denotes |
by |
T7501 |
1109-1110 |
DT |
denotes |
a |
T7502 |
1116-1123 |
NN |
denotes |
syringe |
T7503 |
1111-1112 |
CD |
denotes |
1 |
T7504 |
1113-1115 |
NN |
denotes |
ml |
T7505 |
1124-1131 |
IN |
denotes |
without |
T7506 |
1132-1137 |
VBG |
denotes |
using |
T7507 |
1138-1139 |
DT |
denotes |
a |
T7508 |
1140-1146 |
NN |
denotes |
needle |
T7509 |
1147-1150 |
CC |
denotes |
and |
T7510 |
1151-1162 |
RB |
denotes |
immediately |
T7511 |
1163-1169 |
VBN |
denotes |
frozen |
T7512 |
1170-1172 |
IN |
denotes |
in |
T7513 |
1173-1179 |
JJ |
denotes |
liquid |
T7514 |
1180-1188 |
NN |
denotes |
nitrogen |
T7515 |
1189-1192 |
IN |
denotes |
for |
T7516 |
1193-1200 |
JJ |
denotes |
further |
T7517 |
1201-1204 |
NN |
denotes |
use |
T7518 |
1204-1205 |
. |
denotes |
. |
R4431 |
T7273 |
T7274 |
npadvmod |
Self,forming |
R4432 |
T7274 |
T7275 |
amod |
forming,gradients |
R4433 |
T7276 |
T7275 |
compound |
iodixanol,gradients |
R4434 |
T7277 |
T7275 |
compound |
density,gradients |
R4435 |
T7278 |
T7275 |
prep |
of,gradients |
R4436 |
T7279 |
T7280 |
amod |
human,cells |
R4437 |
T7280 |
T7278 |
pobj |
cells,of |
R4438 |
T7281 |
T7280 |
amod |
red,cells |
R4439 |
T7282 |
T7280 |
compound |
blood,cells |
R4440 |
T7284 |
T7285 |
det |
The,distribution |
R4441 |
T7285 |
T7287 |
nsubjpass |
distribution,addressed |
R4442 |
T7286 |
T7285 |
amod |
subcellular,distribution |
R4443 |
T7288 |
T7285 |
prep |
of,distribution |
R4444 |
T7289 |
T7290 |
compound |
annexin,A7 |
R4445 |
T7290 |
T7288 |
pobj |
A7,of |
R4446 |
T7291 |
T7287 |
auxpass |
was,addressed |
R4447 |
T7292 |
T7287 |
prep |
according,addressed |
R4448 |
T7293 |
T7292 |
prep |
to,according |
R4449 |
T7294 |
T7293 |
punct |
[,to |
R4450 |
T7295 |
T7293 |
pobj |
24,to |
R4451 |
T7296 |
T7287 |
punct |
],addressed |
R4452 |
T7297 |
T7287 |
punct |
.,addressed |
R4453 |
T7299 |
T7300 |
amod |
Red,cells |
R4454 |
T7300 |
T7302 |
nsubjpass |
cells,centrifuged |
R4455 |
T7301 |
T7300 |
compound |
blood,cells |
R4456 |
T7303 |
T7302 |
auxpass |
were,centrifuged |
R4457 |
T7304 |
T7302 |
cc |
and,centrifuged |
R4458 |
T7305 |
T7302 |
conj |
washed,centrifuged |
R4459 |
T7306 |
T7305 |
prep |
in,washed |
R4460 |
T7307 |
T7308 |
amod |
isotonic,solution |
R4461 |
T7308 |
T7306 |
pobj |
solution,in |
R4462 |
T7309 |
T7310 |
amod |
buffered,salt |
R4463 |
T7310 |
T7308 |
compound |
salt,solution |
R4464 |
T7311 |
T7305 |
punct |
", ",washed |
R4465 |
T7312 |
T7305 |
conj |
collected,washed |
R4466 |
T7313 |
T7312 |
prep |
in,collected |
R4467 |
T7314 |
T7315 |
npadvmod |
ice,cold |
R4468 |
T7315 |
T7316 |
amod |
cold,buffer |
R4469 |
T7316 |
T7313 |
pobj |
buffer,in |
R4470 |
T7317 |
T7316 |
compound |
lysis,buffer |
R4471 |
T7318 |
T7319 |
punct |
(,Hepes |
R4472 |
T7319 |
T7316 |
parataxis |
Hepes,buffer |
R4473 |
T7320 |
T7321 |
nummod |
20,mM |
R4474 |
T7321 |
T7319 |
compound |
mM,Hepes |
R4475 |
T7322 |
T7319 |
punct |
", ",Hepes |
R4476 |
T7323 |
T7319 |
npadvmod |
pH,Hepes |
R4477 |
T7324 |
T7323 |
nummod |
7.4,pH |
R4478 |
T7325 |
T7319 |
punct |
", ",Hepes |
R4479 |
T7326 |
T7327 |
nummod |
200,μM |
R4480 |
T7327 |
T7328 |
compound |
μM,CaCl2 |
R4481 |
T7328 |
T7319 |
conj |
CaCl2,Hepes |
R4482 |
T7329 |
T7328 |
punct |
", ",CaCl2 |
R4483 |
T7330 |
T7331 |
nummod |
0.25,M |
R4484 |
T7331 |
T7332 |
compound |
M,sucrose |
R4485 |
T7332 |
T7328 |
conj |
sucrose,CaCl2 |
R4486 |
T7333 |
T7332 |
punct |
", ",sucrose |
R4487 |
T7334 |
T7332 |
cc |
and,sucrose |
R4488 |
T7335 |
T7336 |
compound |
proteinase,inhibitors |
R4489 |
T7336 |
T7332 |
conj |
inhibitors,sucrose |
R4490 |
T7337 |
T7338 |
punct |
(,Roche |
R4491 |
T7338 |
T7336 |
parataxis |
Roche,inhibitors |
R4492 |
T7339 |
T7338 |
punct |
),Roche |
R4493 |
T7340 |
T7336 |
punct |
),inhibitors |
R4494 |
T7341 |
T7312 |
punct |
", ",collected |
R4495 |
T7342 |
T7312 |
conj |
homogenized,collected |
R4496 |
T7343 |
T7342 |
prep |
by,homogenized |
R4497 |
T7344 |
T7345 |
det |
a,homogenizer |
R4498 |
T7345 |
T7343 |
pobj |
homogenizer,by |
R4499 |
T7346 |
T7347 |
advmod |
loosely,fitting |
R4500 |
T7347 |
T7345 |
amod |
fitting,homogenizer |
R4501 |
T7348 |
T7345 |
compound |
dounce,homogenizer |
R4502 |
T7349 |
T7345 |
cc |
and,homogenizer |
R4503 |
T7350 |
T7351 |
amod |
additional,passages |
R4504 |
T7351 |
T7345 |
conj |
passages,homogenizer |
R4505 |
T7352 |
T7351 |
prep |
through,passages |
R4506 |
T7353 |
T7354 |
det |
a,needle |
R4507 |
T7354 |
T7352 |
pobj |
needle,through |
R4508 |
T7355 |
T7356 |
nummod |
22,gauge |
R4509 |
T7356 |
T7354 |
compound |
gauge,needle |
R4510 |
T7357 |
T7302 |
punct |
.,centrifuged |
R4511 |
T7359 |
T7360 |
det |
A,solution |
R4512 |
T7360 |
T7364 |
nsubjpass |
solution,established |
R4513 |
T7361 |
T7362 |
nummod |
50,% |
R4514 |
T7362 |
T7360 |
compound |
%,solution |
R4515 |
T7363 |
T7360 |
compound |
iodixanol,solution |
R4516 |
T7365 |
T7364 |
auxpass |
was,established |
R4517 |
T7366 |
T7364 |
prep |
by,established |
R4518 |
T7367 |
T7366 |
pcomp |
mixing,by |
R4519 |
T7368 |
T7369 |
compound |
Optiprep,solution |
R4520 |
T7369 |
T7367 |
dobj |
solution,mixing |
R4521 |
T7370 |
T7371 |
punct |
(,Sigma |
R4522 |
T7371 |
T7369 |
parataxis |
Sigma,solution |
R4523 |
T7372 |
T7373 |
nummod |
60,% |
R4524 |
T7373 |
T7374 |
compound |
%,iodixanol |
R4525 |
T7374 |
T7371 |
dep |
iodixanol,Sigma |
R4526 |
T7375 |
T7371 |
punct |
;,Sigma |
R4527 |
T7376 |
T7371 |
punct |
),Sigma |
R4528 |
T7377 |
T7367 |
prep |
with,mixing |
R4529 |
T7378 |
T7377 |
pobj |
buffer,with |
R4530 |
T7379 |
T7380 |
punct |
(,sucrose |
R4531 |
T7380 |
T7378 |
parataxis |
sucrose,buffer |
R4532 |
T7381 |
T7382 |
nummod |
120,mM |
R4533 |
T7382 |
T7383 |
compound |
mM,Hepes |
R4534 |
T7383 |
T7380 |
dep |
Hepes,sucrose |
R4535 |
T7384 |
T7383 |
punct |
", ",Hepes |
R4536 |
T7385 |
T7383 |
npadvmod |
pH,Hepes |
R4537 |
T7386 |
T7385 |
nummod |
7.4,pH |
R4538 |
T7387 |
T7380 |
punct |
", ",sucrose |
R4539 |
T7388 |
T7389 |
nummod |
1.2,mM |
R4540 |
T7389 |
T7390 |
compound |
mM,CaCl2 |
R4541 |
T7390 |
T7380 |
dep |
CaCl2,sucrose |
R4542 |
T7391 |
T7380 |
punct |
", ",sucrose |
R4543 |
T7392 |
T7393 |
nummod |
0.25,M |
R4544 |
T7393 |
T7380 |
compound |
M,sucrose |
R4545 |
T7394 |
T7380 |
punct |
),sucrose |
R4546 |
T7395 |
T7364 |
punct |
.,established |
R4547 |
T7397 |
T7398 |
nummod |
Seven,parts |
R4548 |
T7398 |
T7399 |
nsubjpass |
parts,mixed |
R4549 |
T7400 |
T7398 |
prep |
of,parts |
R4550 |
T7401 |
T7402 |
det |
this,solution |
R4551 |
T7402 |
T7400 |
pobj |
solution,of |
R4552 |
T7403 |
T7404 |
nummod |
50,% |
R4553 |
T7404 |
T7402 |
compound |
%,solution |
R4554 |
T7405 |
T7402 |
compound |
iodixanol,solution |
R4555 |
T7406 |
T7399 |
auxpass |
were,mixed |
R4556 |
T7407 |
T7399 |
prep |
with,mixed |
R4557 |
T7408 |
T7409 |
nummod |
thirteen,parts |
R4558 |
T7409 |
T7407 |
pobj |
parts,with |
R4559 |
T7410 |
T7409 |
prep |
of,parts |
R4560 |
T7411 |
T7412 |
det |
the,solution |
R4561 |
T7412 |
T7410 |
pobj |
solution,of |
R4562 |
T7413 |
T7414 |
amod |
red,cell |
R4563 |
T7414 |
T7412 |
compound |
cell,solution |
R4564 |
T7415 |
T7414 |
compound |
blood,cell |
R4565 |
T7416 |
T7399 |
punct |
", ",mixed |
R4566 |
T7417 |
T7399 |
advcl |
generating,mixed |
R4567 |
T7418 |
T7419 |
det |
a,solution |
R4568 |
T7419 |
T7417 |
dobj |
solution,generating |
R4569 |
T7420 |
T7419 |
compound |
gradient,solution |
R4570 |
T7421 |
T7419 |
acl |
containing,solution |
R4571 |
T7422 |
T7423 |
nummod |
17.5,% |
R4572 |
T7423 |
T7424 |
compound |
%,iodixanol |
R4573 |
T7424 |
T7421 |
dobj |
iodixanol,containing |
R4574 |
T7425 |
T7399 |
punct |
.,mixed |
R4575 |
T7427 |
T7428 |
det |
The,mixture |
R4576 |
T7428 |
T7429 |
nsubj |
mixture,filled |
R4577 |
T7430 |
T7429 |
aux |
was,filled |
R4578 |
T7431 |
T7429 |
prep |
into,filled |
R4579 |
T7432 |
T7433 |
det |
a,tube |
R4580 |
T7433 |
T7431 |
pobj |
tube,into |
R4581 |
T7434 |
T7433 |
amod |
centrifuge,tube |
R4582 |
T7435 |
T7433 |
amod |
underlayed,tube |
R4583 |
T7436 |
T7435 |
prep |
by,underlayed |
R4584 |
T7437 |
T7436 |
pobj |
cushions,by |
R4585 |
T7438 |
T7437 |
prep |
of,cushions |
R4586 |
T7439 |
T7440 |
nummod |
30,% |
R4587 |
T7440 |
T7441 |
nmod |
%,iodixanol |
R4588 |
T7441 |
T7438 |
pobj |
iodixanol,of |
R4589 |
T7442 |
T7440 |
cc |
and,% |
R4590 |
T7443 |
T7444 |
nummod |
35,% |
R4591 |
T7444 |
T7440 |
conj |
%,% |
R4592 |
T7445 |
T7429 |
cc |
and,filled |
R4593 |
T7446 |
T7429 |
conj |
centrifuged,filled |
R4594 |
T7447 |
T7446 |
prep |
for,centrifuged |
R4595 |
T7448 |
T7449 |
nummod |
3,hours |
R4596 |
T7449 |
T7447 |
pobj |
hours,for |
R4597 |
T7450 |
T7446 |
prep |
at,centrifuged |
R4598 |
T7451 |
T7452 |
nummod |
"270,000",g |
R4599 |
T7452 |
T7450 |
pobj |
g,at |
R4600 |
T7453 |
T7452 |
punct |
×,g |
R4601 |
T7454 |
T7446 |
prep |
in,centrifuged |
R4602 |
T7455 |
T7456 |
det |
a,rotor |
R4603 |
T7456 |
T7454 |
pobj |
rotor,in |
R4604 |
T7457 |
T7458 |
compound |
SW41,Ti |
R4605 |
T7458 |
T7456 |
compound |
Ti,rotor |
R4606 |
T7459 |
T7460 |
compound |
swing,out |
R4607 |
T7460 |
T7456 |
compound |
out,rotor |
R4608 |
T7461 |
T7462 |
punct |
(,Beckman |
R4609 |
T7462 |
T7456 |
parataxis |
Beckman,rotor |
R4610 |
T7463 |
T7462 |
punct |
),Beckman |
R4611 |
T7464 |
T7465 |
aux |
to,achieve |
R4612 |
T7465 |
T7429 |
advcl |
achieve,filled |
R4613 |
T7466 |
T7467 |
det |
a,gradient |
R4614 |
T7467 |
T7465 |
dobj |
gradient,achieve |
R4615 |
T7468 |
T7469 |
advmod |
nearly,linear |
R4616 |
T7469 |
T7467 |
amod |
linear,gradient |
R4617 |
T7470 |
T7467 |
compound |
density,gradient |
R4618 |
T7471 |
T7472 |
punct |
(,g |
R4619 |
T7472 |
T7467 |
parataxis |
g,gradient |
R4620 |
T7473 |
T7472 |
advmod |
approximately,g |
R4621 |
T7474 |
T7472 |
nummod |
1.06,g |
R4622 |
T7475 |
T7476 |
punct |
/,ml |
R4623 |
T7476 |
T7472 |
prep |
ml,g |
R4624 |
T7477 |
T7472 |
prep |
to,g |
R4625 |
T7478 |
T7479 |
nummod |
1.20,g |
R4626 |
T7479 |
T7477 |
pobj |
g,to |
R4627 |
T7480 |
T7481 |
punct |
/,ml |
R4628 |
T7481 |
T7479 |
prep |
ml,g |
R4629 |
T7482 |
T7472 |
punct |
),g |
R4630 |
T7483 |
T7484 |
punct |
[,24 |
R4631 |
T7484 |
T7429 |
parataxis |
24,filled |
R4632 |
T7485 |
T7484 |
punct |
],24 |
R4633 |
T7486 |
T7429 |
punct |
.,filled |
R4634 |
T7488 |
T7489 |
nsubjpass |
Fractions,collected |
R4635 |
T7490 |
T7488 |
prep |
of,Fractions |
R4636 |
T7491 |
T7492 |
nummod |
0.6,ml |
R4637 |
T7492 |
T7490 |
pobj |
ml,of |
R4638 |
T7493 |
T7489 |
auxpass |
were,collected |
R4639 |
T7494 |
T7489 |
prep |
from,collected |
R4640 |
T7495 |
T7496 |
det |
the,top |
R4641 |
T7496 |
T7494 |
pobj |
top,from |
R4642 |
T7497 |
T7496 |
prep |
of,top |
R4643 |
T7498 |
T7499 |
det |
the,gradient |
R4644 |
T7499 |
T7497 |
pobj |
gradient,of |
R4645 |
T7500 |
T7489 |
prep |
by,collected |
R4646 |
T7501 |
T7502 |
det |
a,syringe |
R4647 |
T7502 |
T7500 |
pobj |
syringe,by |
R4648 |
T7503 |
T7504 |
nummod |
1,ml |
R4649 |
T7504 |
T7502 |
compound |
ml,syringe |
R4650 |
T7505 |
T7489 |
prep |
without,collected |
R4651 |
T7506 |
T7505 |
pcomp |
using,without |
R4652 |
T7507 |
T7508 |
det |
a,needle |
R4653 |
T7508 |
T7506 |
dobj |
needle,using |
R4654 |
T7509 |
T7489 |
cc |
and,collected |
R4655 |
T7510 |
T7511 |
advmod |
immediately,frozen |
R4656 |
T7511 |
T7489 |
conj |
frozen,collected |
R4657 |
T7512 |
T7511 |
prep |
in,frozen |
R4658 |
T7513 |
T7514 |
amod |
liquid,nitrogen |
R4659 |
T7514 |
T7512 |
pobj |
nitrogen,in |
R4660 |
T7515 |
T7511 |
prep |
for,frozen |
R4661 |
T7516 |
T7517 |
amod |
further,use |
R4662 |
T7517 |
T7515 |
pobj |
use,for |
R4663 |
T7518 |
T7489 |
punct |
.,collected |