PMC:194730 / 26355-27097 JSONTXT

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    craft-sa-dev

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this paper we report the presence of both annexin A7 isoforms in red blood cells where they are abundant in nanovesicles that form upon Ca2+ addition. The proteins are however not required for the vesiculation process. They are also not essential for the formation of specific membrane domains such as lipid rafts, as exogenously added recombinant annexin A7 redistributed to similar positions in a density gradient containing membranes derived from red blood cells of anxA7-/-mice. The observed cell shape change of erythrocytes lacking annexin A7 might be due to alterations in the erythrocyte cytoskeleton. As a direct interaction with F-actin was ruled out, this effect might be mediated by annexin's Ca2+-binding activity."}

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T6590","span":{"begin":57,"end":67},"obj":"PR:000004080"},{"id":"T6591","span":{"begin":68,"end":76},"obj":"SO:0001060"},{"id":"T6592","span":{"begin":80,"end":95},"obj":"CL:0000232"},{"id":"T6593","span":{"begin":84,"end":89},"obj":"UBERON:0000178"},{"id":"T6594","span":{"begin":127,"end":135},"obj":"GO:0031982"},{"id":"T6595","span":{"begin":151,"end":155},"obj":"CHEBI:29108"},{"id":"T6596","span":{"begin":212,"end":224},"obj":"GO:0006900"},{"id":"T6597","span":{"begin":292,"end":308},"obj":"GO:0044425"},{"id":"T6598","span":{"begin":317,"end":322},"obj":"CHEBI:18059"},{"id":"T6599","span":{"begin":317,"end":328},"obj":"GO:0045121"},{"id":"T6600","span":{"begin":363,"end":373},"obj":"PR:000004080"},{"id":"T6601","span":{"begin":442,"end":451},"obj":"GO:0016020"},{"id":"T6602","span":{"begin":465,"end":480},"obj":"CL:0000232"},{"id":"T6603","span":{"begin":469,"end":474},"obj":"UBERON:0000178"},{"id":"T6604","span":{"begin":484,"end":489},"obj":"PR:000004080"},{"id":"T6605","span":{"begin":492,"end":496},"obj":"NCBITaxon:10088"},{"id":"T6606","span":{"begin":532,"end":544},"obj":"CL:0000232"},{"id":"T6607","span":{"begin":553,"end":563},"obj":"PR:000004080"},{"id":"T6608","span":{"begin":599,"end":610},"obj":"CL:0000232"},{"id":"T6609","span":{"begin":611,"end":623},"obj":"GO:0005856"},{"id":"T6610","span":{"begin":654,"end":661},"obj":"GO:0031941"},{"id":"T6611","span":{"begin":720,"end":724},"obj":"CHEBI:29108"}],"text":"Conclusions\nIn this paper we report the presence of both annexin A7 isoforms in red blood cells where they are abundant in nanovesicles that form upon Ca2+ addition. The proteins are however not required for the vesiculation process. They are also not essential for the formation of specific membrane domains such as lipid rafts, as exogenously added recombinant annexin A7 redistributed to similar positions in a density gradient containing membranes derived from red blood cells of anxA7-/-mice. The observed cell shape change of erythrocytes lacking annexin A7 might be due to alterations in the erythrocyte cytoskeleton. As a direct interaction with F-actin was ruled out, this effect might be mediated by annexin's Ca2+-binding activity."}

    craft-ca-core-ex-dev

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