Id |
Subject |
Object |
Predicate |
Lexical cue |
T206 |
86-94 |
NNS |
denotes |
Insights |
T207 |
0-134 |
sentence |
denotes |
Function, expression and localization of annexin A7 in platelets and red blood cells: Insights derived from an annexin A7 mutant mouse |
T208 |
8-10 |
, |
denotes |
, |
T209 |
10-20 |
NN |
denotes |
expression |
T210 |
21-24 |
CC |
denotes |
and |
T211 |
25-37 |
NN |
denotes |
localization |
T212 |
38-40 |
IN |
denotes |
of |
T213 |
41-48 |
NN |
denotes |
annexin |
T214 |
49-51 |
NN |
denotes |
A7 |
T215 |
52-54 |
IN |
denotes |
in |
T216 |
55-64 |
NNS |
denotes |
platelets |
T217 |
65-68 |
CC |
denotes |
and |
T218 |
69-72 |
JJ |
denotes |
red |
T219 |
79-84 |
NNS |
denotes |
cells |
T220 |
73-78 |
NN |
denotes |
blood |
T221 |
84-86 |
: |
denotes |
: |
T222 |
95-102 |
VBN |
denotes |
derived |
T223 |
103-107 |
IN |
denotes |
from |
T224 |
108-110 |
DT |
denotes |
an |
T225 |
129-134 |
NN |
denotes |
mouse |
T226 |
111-118 |
NN |
denotes |
annexin |
T227 |
119-121 |
NN |
denotes |
A7 |
T228 |
122-128 |
JJ |
denotes |
mutant |
T229 |
134-135 |
sentence |
denotes |
|
T230 |
145-155 |
sentence |
denotes |
Background |
T231 |
145-155 |
NN |
denotes |
Background |
T232 |
155-310 |
sentence |
denotes |
Annexin A7 is a Ca2+- and phospholipid-binding protein expressed as a 47 and 51 kDa isoform, which is thought to be involved in membrane fusion processes. |
T233 |
156-163 |
NN |
denotes |
Annexin |
T234 |
164-166 |
NN |
denotes |
A7 |
T235 |
167-169 |
VBZ |
denotes |
is |
T236 |
170-171 |
DT |
denotes |
a |
T237 |
203-210 |
NN |
denotes |
protein |
T238 |
172-176 |
NN |
denotes |
Ca2+ |
T239 |
195-202 |
VBG |
denotes |
binding |
T240 |
176-177 |
HYPH |
denotes |
- |
T241 |
178-181 |
CC |
denotes |
and |
T242 |
182-194 |
NN |
denotes |
phospholipid |
T243 |
194-195 |
HYPH |
denotes |
- |
T244 |
211-220 |
VBN |
denotes |
expressed |
T245 |
221-223 |
IN |
denotes |
as |
T246 |
224-225 |
DT |
denotes |
a |
T247 |
240-247 |
NN |
denotes |
isoform |
T248 |
226-228 |
CD |
denotes |
47 |
T249 |
236-239 |
NN |
denotes |
kDa |
T250 |
229-232 |
CC |
denotes |
and |
T251 |
233-235 |
CD |
denotes |
51 |
T252 |
247-249 |
, |
denotes |
, |
T253 |
249-254 |
WDT |
denotes |
which |
T254 |
258-265 |
VBN |
denotes |
thought |
T255 |
255-257 |
VBZ |
denotes |
is |
T256 |
266-268 |
TO |
denotes |
to |
T257 |
272-280 |
VBN |
denotes |
involved |
T258 |
269-271 |
VB |
denotes |
be |
T259 |
281-283 |
IN |
denotes |
in |
T260 |
284-292 |
NN |
denotes |
membrane |
T261 |
293-299 |
NN |
denotes |
fusion |
T262 |
300-309 |
NNS |
denotes |
processes |
T263 |
309-310 |
. |
denotes |
. |
T264 |
310-590 |
sentence |
denotes |
Recently the 47 kDa isoform has been identified in erythrocytes where it was proposed to be a key component in the process of the Ca2+-dependent vesicle release, a process with which red blood cells might protect themselves against an attack by for example complement components. |
T265 |
311-319 |
RB |
denotes |
Recently |
T266 |
348-358 |
VBN |
denotes |
identified |
T267 |
320-323 |
DT |
denotes |
the |
T268 |
331-338 |
NN |
denotes |
isoform |
T269 |
324-326 |
CD |
denotes |
47 |
T270 |
327-330 |
NN |
denotes |
kDa |
T271 |
339-342 |
VBZ |
denotes |
has |
T272 |
343-347 |
VBN |
denotes |
been |
T273 |
359-361 |
IN |
denotes |
in |
T274 |
362-374 |
NNS |
denotes |
erythrocytes |
T275 |
375-380 |
WRB |
denotes |
where |
T276 |
388-396 |
VBN |
denotes |
proposed |
T277 |
381-383 |
PRP |
denotes |
it |
T278 |
384-387 |
VBD |
denotes |
was |
T279 |
397-399 |
TO |
denotes |
to |
T280 |
400-402 |
VB |
denotes |
be |
T281 |
403-404 |
DT |
denotes |
a |
T282 |
409-418 |
NN |
denotes |
component |
T283 |
405-408 |
JJ |
denotes |
key |
T284 |
419-421 |
IN |
denotes |
in |
T285 |
422-425 |
DT |
denotes |
the |
T286 |
426-433 |
NN |
denotes |
process |
T287 |
434-436 |
IN |
denotes |
of |
T288 |
437-440 |
DT |
denotes |
the |
T289 |
464-471 |
NN |
denotes |
release |
T290 |
441-445 |
NN |
denotes |
Ca2+ |
T291 |
446-455 |
JJ |
denotes |
dependent |
T292 |
445-446 |
HYPH |
denotes |
- |
T293 |
456-463 |
NN |
denotes |
vesicle |
T294 |
471-473 |
, |
denotes |
, |
T295 |
473-474 |
DT |
denotes |
a |
T296 |
475-482 |
NN |
denotes |
process |
T297 |
483-487 |
IN |
denotes |
with |
T298 |
516-523 |
VB |
denotes |
protect |
T299 |
488-493 |
WDT |
denotes |
which |
T300 |
494-497 |
JJ |
denotes |
red |
T301 |
504-509 |
NNS |
denotes |
cells |
T302 |
498-503 |
NN |
denotes |
blood |
T303 |
510-515 |
MD |
denotes |
might |
T304 |
524-534 |
PRP |
denotes |
themselves |
T305 |
535-542 |
IN |
denotes |
against |
T306 |
543-545 |
DT |
denotes |
an |
T307 |
546-552 |
NN |
denotes |
attack |
T308 |
553-555 |
IN |
denotes |
by |
T309 |
556-559 |
IN |
denotes |
for |
T310 |
579-589 |
NNS |
denotes |
components |
T311 |
560-567 |
NN |
denotes |
example |
T312 |
568-578 |
NN |
denotes |
complement |
T313 |
589-590 |
. |
denotes |
. |
T314 |
590-599 |
sentence |
denotes |
Results |
T315 |
592-599 |
NNS |
denotes |
Results |
T316 |
599-691 |
sentence |
denotes |
The role of annexin A7 in red blood cells was addressed in erythrocytes from anxA7-/- mice. |
T317 |
600-603 |
DT |
denotes |
The |
T318 |
604-608 |
NN |
denotes |
role |
T319 |
646-655 |
VBN |
denotes |
addressed |
T320 |
609-611 |
IN |
denotes |
of |
T321 |
612-619 |
NN |
denotes |
annexin |
T322 |
620-622 |
NN |
denotes |
A7 |
T323 |
623-625 |
IN |
denotes |
in |
T324 |
626-629 |
JJ |
denotes |
red |
T325 |
636-641 |
NNS |
denotes |
cells |
T326 |
630-635 |
NN |
denotes |
blood |
T327 |
642-645 |
VBD |
denotes |
was |
T328 |
656-658 |
IN |
denotes |
in |
T329 |
659-671 |
NNS |
denotes |
erythrocytes |
T330 |
672-676 |
IN |
denotes |
from |
T331 |
677-682 |
JJ |
denotes |
anxA7 |
T332 |
686-690 |
NNS |
denotes |
mice |
T333 |
682-683 |
SYM |
denotes |
- |
T334 |
683-684 |
HYPH |
denotes |
/ |
T335 |
684-685 |
SYM |
denotes |
- |
T336 |
690-691 |
. |
denotes |
. |
T337 |
691-763 |
sentence |
denotes |
Interestingly, the Ca2+-mediated vesiculation process was not impaired. |
T338 |
692-705 |
RB |
denotes |
Interestingly |
T339 |
754-762 |
VBN |
denotes |
impaired |
T340 |
705-707 |
, |
denotes |
, |
T341 |
707-710 |
DT |
denotes |
the |
T342 |
738-745 |
NN |
denotes |
process |
T343 |
711-715 |
NN |
denotes |
Ca2+ |
T344 |
716-724 |
VBN |
denotes |
mediated |
T345 |
715-716 |
HYPH |
denotes |
- |
T346 |
725-737 |
NN |
denotes |
vesiculation |
T347 |
746-749 |
VBD |
denotes |
was |
T348 |
750-753 |
RB |
denotes |
not |
T349 |
762-763 |
. |
denotes |
. |
T350 |
763-874 |
sentence |
denotes |
Also, the membrane organization appeared not to be disturbed as assessed using gradient fractionation studies. |
T351 |
764-768 |
RB |
denotes |
Also |
T352 |
796-804 |
VBD |
denotes |
appeared |
T353 |
768-770 |
, |
denotes |
, |
T354 |
770-773 |
DT |
denotes |
the |
T355 |
783-795 |
NN |
denotes |
organization |
T356 |
774-782 |
NN |
denotes |
membrane |
T357 |
805-808 |
RB |
denotes |
not |
T358 |
815-824 |
VBN |
denotes |
disturbed |
T359 |
809-811 |
TO |
denotes |
to |
T360 |
812-814 |
VB |
denotes |
be |
T361 |
825-827 |
IN |
denotes |
as |
T362 |
828-836 |
VBN |
denotes |
assessed |
T363 |
837-842 |
VBG |
denotes |
using |
T364 |
843-851 |
NN |
denotes |
gradient |
T365 |
852-865 |
NN |
denotes |
fractionation |
T366 |
866-873 |
NNS |
denotes |
studies |
T367 |
873-874 |
. |
denotes |
. |
T368 |
874-984 |
sentence |
denotes |
Instead, lack of annexin A7 led to an altered cell shape and increased osmotic resistance of red blood cells. |
T369 |
875-882 |
RB |
denotes |
Instead |
T370 |
903-906 |
VBD |
denotes |
led |
T371 |
882-884 |
, |
denotes |
, |
T372 |
884-888 |
NN |
denotes |
lack |
T373 |
889-891 |
IN |
denotes |
of |
T374 |
892-899 |
NN |
denotes |
annexin |
T375 |
900-902 |
NN |
denotes |
A7 |
T376 |
907-909 |
IN |
denotes |
to |
T377 |
910-912 |
DT |
denotes |
an |
T378 |
926-931 |
NN |
denotes |
shape |
T379 |
913-920 |
JJ |
denotes |
altered |
T380 |
921-925 |
NN |
denotes |
cell |
T381 |
932-935 |
CC |
denotes |
and |
T382 |
936-945 |
VBN |
denotes |
increased |
T383 |
954-964 |
NN |
denotes |
resistance |
T384 |
946-953 |
JJ |
denotes |
osmotic |
T385 |
965-967 |
IN |
denotes |
of |
T386 |
968-971 |
JJ |
denotes |
red |
T387 |
978-983 |
NNS |
denotes |
cells |
T388 |
972-977 |
NN |
denotes |
blood |
T389 |
983-984 |
. |
denotes |
. |
T390 |
984-1029 |
sentence |
denotes |
Annexin A7 was also identified in platelets. |
T391 |
985-992 |
NN |
denotes |
Annexin |
T392 |
993-995 |
NN |
denotes |
A7 |
T393 |
1005-1015 |
VBN |
denotes |
identified |
T394 |
996-999 |
VBD |
denotes |
was |
T395 |
1000-1004 |
RB |
denotes |
also |
T396 |
1016-1018 |
IN |
denotes |
in |
T397 |
1019-1028 |
NNS |
denotes |
platelets |
T398 |
1028-1029 |
. |
denotes |
. |
T399 |
1029-1166 |
sentence |
denotes |
In these cells its loss led to a slightly slower aggregation velocity which seems to be compensated by an increased number of platelets. |
T400 |
1030-1032 |
IN |
denotes |
In |
T401 |
1054-1057 |
VBD |
denotes |
led |
T402 |
1033-1038 |
DT |
denotes |
these |
T403 |
1039-1044 |
NNS |
denotes |
cells |
T404 |
1045-1048 |
PRP$ |
denotes |
its |
T405 |
1049-1053 |
NN |
denotes |
loss |
T406 |
1058-1060 |
IN |
denotes |
to |
T407 |
1061-1062 |
DT |
denotes |
a |
T408 |
1091-1099 |
NN |
denotes |
velocity |
T409 |
1063-1071 |
RB |
denotes |
slightly |
T410 |
1072-1078 |
JJR |
denotes |
slower |
T411 |
1079-1090 |
NN |
denotes |
aggregation |
T412 |
1100-1105 |
WDT |
denotes |
which |
T413 |
1106-1111 |
VBZ |
denotes |
seems |
T414 |
1112-1114 |
TO |
denotes |
to |
T415 |
1118-1129 |
VBN |
denotes |
compensated |
T416 |
1115-1117 |
VB |
denotes |
be |
T417 |
1130-1132 |
IN |
denotes |
by |
T418 |
1133-1135 |
DT |
denotes |
an |
T419 |
1146-1152 |
NN |
denotes |
number |
T420 |
1136-1145 |
VBN |
denotes |
increased |
T421 |
1153-1155 |
IN |
denotes |
of |
T422 |
1156-1165 |
NNS |
denotes |
platelets |
T423 |
1165-1166 |
. |
denotes |
. |
T424 |
1166-1288 |
sentence |
denotes |
The results appear to rule out an important role of annexin A7 in membrane fusion processes occurring in red blood cells. |
T425 |
1167-1170 |
DT |
denotes |
The |
T426 |
1171-1178 |
NNS |
denotes |
results |
T427 |
1179-1185 |
VBP |
denotes |
appear |
T428 |
1186-1188 |
TO |
denotes |
to |
T429 |
1189-1193 |
VB |
denotes |
rule |
T430 |
1194-1197 |
RP |
denotes |
out |
T431 |
1198-1200 |
DT |
denotes |
an |
T432 |
1211-1215 |
NN |
denotes |
role |
T433 |
1201-1210 |
JJ |
denotes |
important |
T434 |
1216-1218 |
IN |
denotes |
of |
T435 |
1219-1226 |
NN |
denotes |
annexin |
T436 |
1227-1229 |
NN |
denotes |
A7 |
T437 |
1230-1232 |
IN |
denotes |
in |
T438 |
1233-1241 |
NN |
denotes |
membrane |
T439 |
1242-1248 |
NN |
denotes |
fusion |
T440 |
1249-1258 |
NNS |
denotes |
processes |
T441 |
1259-1268 |
VBG |
denotes |
occurring |
T442 |
1269-1271 |
IN |
denotes |
in |
T443 |
1272-1275 |
JJ |
denotes |
red |
T444 |
1282-1287 |
NNS |
denotes |
cells |
T445 |
1276-1281 |
NN |
denotes |
blood |
T446 |
1287-1288 |
. |
denotes |
. |
T447 |
1288-1376 |
sentence |
denotes |
Instead the protein might be involved in the organization of the membrane cytoskeleton. |
T448 |
1289-1296 |
RB |
denotes |
Instead |
T449 |
1318-1326 |
VBN |
denotes |
involved |
T450 |
1297-1300 |
DT |
denotes |
the |
T451 |
1301-1308 |
NN |
denotes |
protein |
T452 |
1309-1314 |
MD |
denotes |
might |
T453 |
1315-1317 |
VB |
denotes |
be |
T454 |
1327-1329 |
IN |
denotes |
in |
T455 |
1330-1333 |
DT |
denotes |
the |
T456 |
1334-1346 |
NN |
denotes |
organization |
T457 |
1347-1349 |
IN |
denotes |
of |
T458 |
1350-1353 |
DT |
denotes |
the |
T459 |
1363-1375 |
NN |
denotes |
cytoskeleton |
T460 |
1354-1362 |
NN |
denotes |
membrane |
T461 |
1375-1376 |
. |
denotes |
. |
T462 |
1376-1482 |
sentence |
denotes |
Red blood cells may represent an appropriate model to study the role of annexin A7 in cellular processes. |
T463 |
1377-1380 |
JJ |
denotes |
Red |
T464 |
1387-1392 |
NNS |
denotes |
cells |
T465 |
1381-1386 |
NN |
denotes |
blood |
T466 |
1397-1406 |
VB |
denotes |
represent |
T467 |
1393-1396 |
MD |
denotes |
may |
T468 |
1407-1409 |
DT |
denotes |
an |
T469 |
1422-1427 |
NN |
denotes |
model |
T470 |
1410-1421 |
JJ |
denotes |
appropriate |
T471 |
1428-1430 |
TO |
denotes |
to |
T472 |
1431-1436 |
VB |
denotes |
study |
T473 |
1437-1440 |
DT |
denotes |
the |
T474 |
1441-1445 |
NN |
denotes |
role |
T475 |
1446-1448 |
IN |
denotes |
of |
T476 |
1449-1456 |
NN |
denotes |
annexin |
T477 |
1457-1459 |
NN |
denotes |
A7 |
T478 |
1460-1462 |
IN |
denotes |
in |
T479 |
1463-1471 |
JJ |
denotes |
cellular |
T480 |
1472-1481 |
NNS |
denotes |
processes |
T481 |
1481-1482 |
. |
denotes |
. |
T482 |
1482-1494 |
sentence |
denotes |
Conclusion |
T483 |
1484-1494 |
NN |
denotes |
Conclusion |
T484 |
1494-1627 |
sentence |
denotes |
We have demonstrated the presence of both annexin A7 isoforms in red blood cells and the presence of the small isoform in platelets. |
T485 |
1495-1497 |
PRP |
denotes |
We |
T486 |
1503-1515 |
VBN |
denotes |
demonstrated |
T487 |
1498-1502 |
VBP |
denotes |
have |
T488 |
1516-1519 |
DT |
denotes |
the |
T489 |
1520-1528 |
NN |
denotes |
presence |
T490 |
1529-1531 |
IN |
denotes |
of |
T491 |
1532-1536 |
CC |
denotes |
both |
T492 |
1548-1556 |
NNS |
denotes |
isoforms |
T493 |
1537-1544 |
NN |
denotes |
annexin |
T494 |
1545-1547 |
NN |
denotes |
A7 |
T495 |
1557-1559 |
IN |
denotes |
in |
T496 |
1560-1563 |
JJ |
denotes |
red |
T497 |
1570-1575 |
NNS |
denotes |
cells |
T498 |
1564-1569 |
NN |
denotes |
blood |
T499 |
1576-1579 |
CC |
denotes |
and |
T500 |
1580-1583 |
DT |
denotes |
the |
T501 |
1584-1592 |
NN |
denotes |
presence |
T502 |
1593-1595 |
IN |
denotes |
of |
T503 |
1596-1599 |
DT |
denotes |
the |
T504 |
1606-1613 |
NN |
denotes |
isoform |
T505 |
1600-1605 |
JJ |
denotes |
small |
T506 |
1614-1616 |
IN |
denotes |
in |
T507 |
1617-1626 |
NNS |
denotes |
platelets |
T508 |
1626-1627 |
. |
denotes |
. |
T509 |
1627-1697 |
sentence |
denotes |
In both cell types the loss of annexin A7 impairs cellular functions. |
T510 |
1628-1630 |
IN |
denotes |
In |
T511 |
1670-1677 |
VBZ |
denotes |
impairs |
T512 |
1631-1635 |
DT |
denotes |
both |
T513 |
1641-1646 |
NNS |
denotes |
types |
T514 |
1636-1640 |
NN |
denotes |
cell |
T515 |
1647-1650 |
DT |
denotes |
the |
T516 |
1651-1655 |
NN |
denotes |
loss |
T517 |
1656-1658 |
IN |
denotes |
of |
T518 |
1659-1666 |
NN |
denotes |
annexin |
T519 |
1667-1669 |
NN |
denotes |
A7 |
T520 |
1678-1686 |
JJ |
denotes |
cellular |
T521 |
1687-1696 |
NNS |
denotes |
functions |
T522 |
1696-1697 |
. |
denotes |
. |
T523 |
1697-1830 |
sentence |
denotes |
The defects observed are however not compatible with a crucial role for annexin A7 in membrane fusion processes in these cell types. |
T524 |
1698-1701 |
DT |
denotes |
The |
T525 |
1702-1709 |
NNS |
denotes |
defects |
T526 |
1719-1722 |
VBP |
denotes |
are |
T527 |
1710-1718 |
VBN |
denotes |
observed |
T528 |
1723-1730 |
RB |
denotes |
however |
T529 |
1731-1734 |
RB |
denotes |
not |
T530 |
1735-1745 |
JJ |
denotes |
compatible |
T531 |
1746-1750 |
IN |
denotes |
with |
T532 |
1751-1752 |
DT |
denotes |
a |
T533 |
1761-1765 |
NN |
denotes |
role |
T534 |
1753-1760 |
JJ |
denotes |
crucial |
T535 |
1766-1769 |
IN |
denotes |
for |
T536 |
1770-1777 |
NN |
denotes |
annexin |
T537 |
1778-1780 |
NN |
denotes |
A7 |
T538 |
1781-1783 |
IN |
denotes |
in |
T539 |
1784-1792 |
NN |
denotes |
membrane |
T540 |
1793-1799 |
NN |
denotes |
fusion |
T541 |
1800-1809 |
NNS |
denotes |
processes |
T542 |
1810-1812 |
IN |
denotes |
in |
T543 |
1813-1818 |
DT |
denotes |
these |
T544 |
1824-1829 |
NNS |
denotes |
types |
T545 |
1819-1823 |
NN |
denotes |
cell |
T546 |
1829-1830 |
. |
denotes |
. |
T1135 |
1851-1853 |
NN |
denotes |
A7 |
T1136 |
1854-1856 |
VBZ |
denotes |
is |
T1137 |
1857-1858 |
DT |
denotes |
a |
T1138 |
1890-1897 |
NN |
denotes |
protein |
T1139 |
1859-1863 |
NN |
denotes |
Ca2+ |
T1140 |
1882-1889 |
VBG |
denotes |
binding |
T1141 |
1863-1864 |
HYPH |
denotes |
- |
T1142 |
1865-1868 |
CC |
denotes |
and |
T1143 |
1869-1881 |
NN |
denotes |
phospholipid |
T1144 |
1881-1882 |
HYPH |
denotes |
- |
T1145 |
1897-1899 |
, |
denotes |
, |
T1146 |
1899-1904 |
WDT |
denotes |
which |
T1147 |
1909-1917 |
VBN |
denotes |
isolated |
T1148 |
1905-1908 |
VBD |
denotes |
was |
T1149 |
1918-1920 |
IN |
denotes |
as |
T1150 |
1921-1924 |
DT |
denotes |
the |
T1151 |
1925-1930 |
NN |
denotes |
agent |
T1152 |
1931-1935 |
WDT |
denotes |
that |
T1153 |
1936-1944 |
VBD |
denotes |
mediated |
T1154 |
1945-1956 |
NN |
denotes |
aggregation |
T1155 |
1957-1959 |
IN |
denotes |
of |
T1156 |
1960-1970 |
NN |
denotes |
chromaffin |
T1157 |
1971-1979 |
NNS |
denotes |
granules |
T1158 |
1980-1983 |
CC |
denotes |
and |
T1159 |
1984-1990 |
NN |
denotes |
fusion |
T1160 |
1991-1993 |
IN |
denotes |
of |
T1161 |
1994-2006 |
NN |
denotes |
phospholipid |
T1162 |
2007-2016 |
NNS |
denotes |
membranes |
T1163 |
2017-2019 |
IN |
denotes |
in |
T1164 |
2020-2023 |
DT |
denotes |
the |
T1165 |
2024-2032 |
NN |
denotes |
presence |
T1166 |
2033-2035 |
IN |
denotes |
of |
T1167 |
2036-2040 |
NN |
denotes |
Ca2+ |
T1168 |
2040-2041 |
. |
denotes |
. |
T1169 |
2041-2154 |
sentence |
denotes |
This activity led to the proposal of annexin A7's involvement in the exocytotic secretion of catecholamines [1]. |
T1170 |
2042-2046 |
DT |
denotes |
This |
T1171 |
2047-2055 |
NN |
denotes |
activity |
T1172 |
2056-2059 |
VBD |
denotes |
led |
T1173 |
2060-2062 |
IN |
denotes |
to |
T1174 |
2063-2066 |
DT |
denotes |
the |
T1175 |
2067-2075 |
NN |
denotes |
proposal |
T1176 |
2076-2078 |
IN |
denotes |
of |
T1177 |
2079-2086 |
NN |
denotes |
annexin |
T1178 |
2087-2089 |
NN |
denotes |
A7 |
T1179 |
2092-2103 |
NN |
denotes |
involvement |
T1180 |
2089-2091 |
POS |
denotes |
's |
T1181 |
2104-2106 |
IN |
denotes |
in |
T1182 |
2107-2110 |
DT |
denotes |
the |
T1183 |
2122-2131 |
NN |
denotes |
secretion |
T1184 |
2111-2121 |
JJ |
denotes |
exocytotic |
T1185 |
2132-2134 |
IN |
denotes |
of |
T1186 |
2135-2149 |
NNS |
denotes |
catecholamines |
T1187 |
2150-2151 |
-LRB- |
denotes |
[ |
T1188 |
2151-2152 |
CD |
denotes |
1 |
T1189 |
2152-2153 |
-RRB- |
denotes |
] |
T1190 |
2153-2154 |
. |
denotes |
. |
T1191 |
2154-2310 |
sentence |
denotes |
The protein belongs to a family of evolutionarily conserved proteins of a bipartite structure with a variable N-terminal and a conserved C-terminal domain. |
T1192 |
2155-2158 |
DT |
denotes |
The |
T1193 |
2159-2166 |
NN |
denotes |
protein |
T1194 |
2167-2174 |
VBZ |
denotes |
belongs |
T1195 |
2175-2177 |
IN |
denotes |
to |
T1196 |
2178-2179 |
DT |
denotes |
a |
T1197 |
2180-2186 |
NN |
denotes |
family |
T1198 |
2187-2189 |
IN |
denotes |
of |
T1199 |
2190-2204 |
RB |
denotes |
evolutionarily |
T1200 |
2205-2214 |
VBN |
denotes |
conserved |
T1201 |
2215-2223 |
NN |
denotes |
proteins |
T1202 |
2224-2226 |
IN |
denotes |
of |
T1203 |
2227-2228 |
DT |
denotes |
a |
T1204 |
2239-2248 |
NN |
denotes |
structure |
T1205 |
2229-2238 |
JJ |
denotes |
bipartite |
T1206 |
2249-2253 |
IN |
denotes |
with |
T1207 |
2254-2255 |
DT |
denotes |
a |
T1208 |
2256-2264 |
JJ |
denotes |
variable |
T1209 |
2265-2266 |
NN |
denotes |
N |
T1210 |
2267-2275 |
JJ |
denotes |
terminal |
T1211 |
2266-2267 |
HYPH |
denotes |
- |
T1212 |
2276-2279 |
CC |
denotes |
and |
T1213 |
2280-2281 |
DT |
denotes |
a |
T1214 |
2282-2291 |
VBN |
denotes |
conserved |
T1215 |
2292-2293 |
NN |
denotes |
C |
T1216 |
2294-2302 |
JJ |
denotes |
terminal |
T1217 |
2293-2294 |
HYPH |
denotes |
- |
T1218 |
2303-2309 |
NN |
denotes |
domain |
T1219 |
2309-2310 |
. |
denotes |
. |
T1220 |
2310-2455 |
sentence |
denotes |
The C-terminal domain is responsible for the Ca2+- and phospholipid-binding, the N-terminal domain appears to confer functional diversity [2-4]. |
T1221 |
2311-2314 |
DT |
denotes |
The |
T1222 |
2326-2332 |
NN |
denotes |
domain |
T1223 |
2315-2316 |
NN |
denotes |
C |
T1224 |
2317-2325 |
JJ |
denotes |
terminal |
T1225 |
2316-2317 |
HYPH |
denotes |
- |
T1226 |
2333-2335 |
VBZ |
denotes |
is |
T1227 |
2410-2417 |
VBZ |
denotes |
appears |
T1228 |
2336-2347 |
JJ |
denotes |
responsible |
T1229 |
2348-2351 |
IN |
denotes |
for |
T1230 |
2352-2355 |
DT |
denotes |
the |
T1231 |
2356-2360 |
NN |
denotes |
Ca2+ |
T1232 |
2360-2361 |
HYPH |
denotes |
- |
T1233 |
2362-2365 |
CC |
denotes |
and |
T1234 |
2366-2378 |
NN |
denotes |
phospholipid |
T1235 |
2378-2379 |
HYPH |
denotes |
- |
T1236 |
2379-2386 |
VBG |
denotes |
binding |
T1237 |
2386-2388 |
, |
denotes |
, |
T1238 |
2388-2391 |
DT |
denotes |
the |
T1239 |
2403-2409 |
NN |
denotes |
domain |
T1240 |
2392-2393 |
NN |
denotes |
N |
T1241 |
2394-2402 |
JJ |
denotes |
terminal |
T1242 |
2393-2394 |
HYPH |
denotes |
- |
T1243 |
2418-2420 |
TO |
denotes |
to |
T1244 |
2421-2427 |
VB |
denotes |
confer |
T1245 |
2428-2438 |
JJ |
denotes |
functional |
T1246 |
2439-2448 |
NN |
denotes |
diversity |
T1247 |
2449-2450 |
-LRB- |
denotes |
[ |
T1248 |
2450-2451 |
CD |
denotes |
2 |
T1249 |
2451-2452 |
SYM |
denotes |
- |
T1250 |
2452-2453 |
CD |
denotes |
4 |
T1251 |
2453-2454 |
-RRB- |
denotes |
] |
T1252 |
2454-2455 |
. |
denotes |
. |
T1253 |
2455-2543 |
sentence |
denotes |
The binding to negatively charged phospholipids is thought to be mediated by Ca2+ ions. |
T1254 |
2456-2459 |
DT |
denotes |
The |
T1255 |
2460-2467 |
NN |
denotes |
binding |
T1256 |
2507-2514 |
VBN |
denotes |
thought |
T1257 |
2468-2470 |
IN |
denotes |
to |
T1258 |
2471-2481 |
RB |
denotes |
negatively |
T1259 |
2482-2489 |
JJ |
denotes |
charged |
T1260 |
2490-2503 |
NNS |
denotes |
phospholipids |
T1261 |
2504-2506 |
VBZ |
denotes |
is |
T1262 |
2515-2517 |
TO |
denotes |
to |
T1263 |
2521-2529 |
VBN |
denotes |
mediated |
T1264 |
2518-2520 |
VB |
denotes |
be |
T1265 |
2530-2532 |
IN |
denotes |
by |
T1266 |
2533-2537 |
NN |
denotes |
Ca2+ |
T1267 |
2538-2542 |
NNS |
denotes |
ions |
T1268 |
2542-2543 |
. |
denotes |
. |
T1269 |
2543-2670 |
sentence |
denotes |
Annexin A7 is unique in that it carries an extraordinarily long and hydrophobic amino terminus with more than 100 amino acids. |
T1270 |
2544-2551 |
NN |
denotes |
Annexin |
T1271 |
2552-2554 |
NN |
denotes |
A7 |
T1272 |
2555-2557 |
VBZ |
denotes |
is |
T1273 |
2558-2564 |
JJ |
denotes |
unique |
T1274 |
2565-2567 |
IN |
denotes |
in |
T1275 |
2576-2583 |
VBZ |
denotes |
carries |
T1276 |
2568-2572 |
IN |
denotes |
that |
T1277 |
2573-2575 |
PRP |
denotes |
it |
T1278 |
2584-2586 |
DT |
denotes |
an |
T1279 |
2630-2638 |
NN |
denotes |
terminus |
T1280 |
2587-2602 |
RB |
denotes |
extraordinarily |
T1281 |
2603-2607 |
JJ |
denotes |
long |
T1282 |
2608-2611 |
CC |
denotes |
and |
T1283 |
2612-2623 |
JJ |
denotes |
hydrophobic |
T1284 |
2624-2629 |
NN |
denotes |
amino |
T1285 |
2639-2643 |
IN |
denotes |
with |
T1286 |
2644-2648 |
JJR |
denotes |
more |
T1287 |
2654-2657 |
CD |
denotes |
100 |
T1288 |
2649-2653 |
IN |
denotes |
than |
T1289 |
2664-2669 |
NNS |
denotes |
acids |
T1290 |
2658-2663 |
NN |
denotes |
amino |
T1291 |
2669-2670 |
. |
denotes |
. |
T1292 |
2670-2736 |
sentence |
denotes |
Alternative splicing gives rise to two isoforms of 47 and 51 kDa. |
T1293 |
2671-2682 |
JJ |
denotes |
Alternative |
T1294 |
2683-2691 |
NN |
denotes |
splicing |
T1295 |
2692-2697 |
VBZ |
denotes |
gives |
T1296 |
2698-2702 |
NN |
denotes |
rise |
T1297 |
2703-2705 |
IN |
denotes |
to |
T1298 |
2706-2709 |
CD |
denotes |
two |
T1299 |
2710-2718 |
NNS |
denotes |
isoforms |
T1300 |
2719-2721 |
IN |
denotes |
of |
T1301 |
2722-2724 |
CD |
denotes |
47 |
T1302 |
2732-2735 |
NN |
denotes |
kDa |
T1303 |
2725-2728 |
CC |
denotes |
and |
T1304 |
2729-2731 |
CD |
denotes |
51 |
T1305 |
2735-2736 |
. |
denotes |
. |
T1306 |
2736-2841 |
sentence |
denotes |
Both isoforms differ by an additional cassette exon located in the first third of the N-terminal domain. |
T1307 |
2737-2741 |
DT |
denotes |
Both |
T1308 |
2742-2750 |
NNS |
denotes |
isoforms |
T1309 |
2751-2757 |
VBP |
denotes |
differ |
T1310 |
2758-2760 |
IN |
denotes |
by |
T1311 |
2761-2763 |
DT |
denotes |
an |
T1312 |
2784-2788 |
NN |
denotes |
exon |
T1313 |
2764-2774 |
JJ |
denotes |
additional |
T1314 |
2775-2783 |
NN |
denotes |
cassette |
T1315 |
2789-2796 |
JJ |
denotes |
located |
T1316 |
2797-2799 |
IN |
denotes |
in |
T1317 |
2800-2803 |
DT |
denotes |
the |
T1318 |
2810-2815 |
NN |
denotes |
third |
T1319 |
2804-2809 |
JJ |
denotes |
first |
T1320 |
2816-2818 |
IN |
denotes |
of |
T1321 |
2819-2822 |
DT |
denotes |
the |
T1322 |
2834-2840 |
NN |
denotes |
domain |
T1323 |
2823-2824 |
NN |
denotes |
N |
T1324 |
2825-2833 |
JJ |
denotes |
terminal |
T1325 |
2824-2825 |
HYPH |
denotes |
- |
T1326 |
2840-2841 |
. |
denotes |
. |
T1327 |
2841-3003 |
sentence |
denotes |
Most tissues harbor only the 47 kDa isoform, both forms are found in brain and heart, while the large isoform is exclusively expressed in mature skeletal muscle. |
T1328 |
2842-2846 |
JJS |
denotes |
Most |
T1329 |
2847-2854 |
NNS |
denotes |
tissues |
T1330 |
2855-2861 |
VBP |
denotes |
harbor |
T1331 |
2902-2907 |
VBN |
denotes |
found |
T1332 |
2862-2866 |
RB |
denotes |
only |
T1333 |
2878-2885 |
NN |
denotes |
isoform |
T1334 |
2867-2870 |
DT |
denotes |
the |
T1335 |
2871-2873 |
CD |
denotes |
47 |
T1336 |
2874-2877 |
NN |
denotes |
kDa |
T1337 |
2885-2887 |
, |
denotes |
, |
T1338 |
2887-2891 |
DT |
denotes |
both |
T1339 |
2892-2897 |
NNS |
denotes |
forms |
T1340 |
2898-2901 |
VBP |
denotes |
are |
T1341 |
2908-2910 |
IN |
denotes |
in |
T1342 |
2911-2916 |
NN |
denotes |
brain |
T1343 |
2917-2920 |
CC |
denotes |
and |
T1344 |
2921-2926 |
NN |
denotes |
heart |
T1345 |
2926-2928 |
, |
denotes |
, |
T1346 |
2928-2933 |
IN |
denotes |
while |
T1347 |
2967-2976 |
VBN |
denotes |
expressed |
T1348 |
2934-2937 |
DT |
denotes |
the |
T1349 |
2944-2951 |
NN |
denotes |
isoform |
T1350 |
2938-2943 |
JJ |
denotes |
large |
T1351 |
2952-2954 |
VBZ |
denotes |
is |
T1352 |
2955-2966 |
RB |
denotes |
exclusively |
T1353 |
2977-2979 |
IN |
denotes |
in |
T1354 |
2980-2986 |
JJ |
denotes |
mature |
T1355 |
2996-3002 |
NN |
denotes |
muscle |
T1356 |
2987-2995 |
JJ |
denotes |
skeletal |
T1357 |
3002-3003 |
. |
denotes |
. |
T1358 |
3003-3209 |
sentence |
denotes |
At the cellular level annexin A7 can be detected in the cytosol, at the plasma membrane, around the nucleus, at vesicular structures including adrenal chromaffin granules, and at the t-tubule system [5,6]. |
T1359 |
3004-3006 |
IN |
denotes |
At |
T1360 |
3044-3052 |
VBN |
denotes |
detected |
T1361 |
3007-3010 |
DT |
denotes |
the |
T1362 |
3020-3025 |
NN |
denotes |
level |
T1363 |
3011-3019 |
JJ |
denotes |
cellular |
T1364 |
3026-3033 |
NN |
denotes |
annexin |
T1365 |
3034-3036 |
NN |
denotes |
A7 |
T1366 |
3037-3040 |
MD |
denotes |
can |
T1367 |
3041-3043 |
VB |
denotes |
be |
T1368 |
3053-3055 |
IN |
denotes |
in |
T1369 |
3056-3059 |
DT |
denotes |
the |
T1370 |
3060-3067 |
NN |
denotes |
cytosol |
T1371 |
3067-3069 |
, |
denotes |
, |
T1372 |
3069-3071 |
IN |
denotes |
at |
T1373 |
3072-3075 |
DT |
denotes |
the |
T1374 |
3083-3091 |
NN |
denotes |
membrane |
T1375 |
3076-3082 |
NN |
denotes |
plasma |
T1376 |
3091-3093 |
, |
denotes |
, |
T1377 |
3093-3099 |
IN |
denotes |
around |
T1378 |
3100-3103 |
DT |
denotes |
the |
T1379 |
3104-3111 |
NN |
denotes |
nucleus |
T1380 |
3111-3113 |
, |
denotes |
, |
T1381 |
3113-3115 |
IN |
denotes |
at |
T1382 |
3116-3125 |
JJ |
denotes |
vesicular |
T1383 |
3126-3136 |
NNS |
denotes |
structures |
T1384 |
3137-3146 |
VBG |
denotes |
including |
T1385 |
3147-3154 |
JJ |
denotes |
adrenal |
T1386 |
3166-3174 |
NNS |
denotes |
granules |
T1387 |
3155-3165 |
NN |
denotes |
chromaffin |
T1388 |
3174-3176 |
, |
denotes |
, |
T1389 |
3176-3179 |
CC |
denotes |
and |
T1390 |
3180-3182 |
IN |
denotes |
at |
T1391 |
3183-3186 |
DT |
denotes |
the |
T1392 |
3196-3202 |
NN |
denotes |
system |
T1393 |
3187-3188 |
NN |
denotes |
t |
T1394 |
3189-3195 |
NN |
denotes |
tubule |
T1395 |
3188-3189 |
HYPH |
denotes |
- |
T1396 |
3203-3204 |
-LRB- |
denotes |
[ |
T1397 |
3206-3207 |
CD |
denotes |
6 |
T1398 |
3204-3205 |
CD |
denotes |
5 |
T1399 |
3205-3206 |
, |
denotes |
, |
T1400 |
3207-3208 |
-RRB- |
denotes |
] |
T1401 |
3208-3209 |
. |
denotes |
. |
T1402 |
3209-3420 |
sentence |
denotes |
Annexin A7 translocates to membranes in a Ca2+-dependent fashion and, when intracellular Ca2+ levels rise, sequentially redistributes to the plasma and the nuclear membrane as well as to intracellular vesicles. |
T1403 |
3210-3217 |
NN |
denotes |
Annexin |
T1404 |
3218-3220 |
NN |
denotes |
A7 |
T1405 |
3221-3233 |
VBZ |
denotes |
translocates |
T1406 |
3234-3236 |
IN |
denotes |
to |
T1407 |
3237-3246 |
NNS |
denotes |
membranes |
T1408 |
3247-3249 |
IN |
denotes |
in |
T1409 |
3250-3251 |
DT |
denotes |
a |
T1410 |
3267-3274 |
NN |
denotes |
fashion |
T1411 |
3252-3256 |
NN |
denotes |
Ca2+ |
T1412 |
3257-3266 |
JJ |
denotes |
dependent |
T1413 |
3256-3257 |
HYPH |
denotes |
- |
T1414 |
3275-3278 |
CC |
denotes |
and |
T1415 |
3278-3280 |
, |
denotes |
, |
T1416 |
3330-3343 |
VBZ |
denotes |
redistributes |
T1417 |
3280-3284 |
WRB |
denotes |
when |
T1418 |
3311-3315 |
VBP |
denotes |
rise |
T1419 |
3285-3298 |
JJ |
denotes |
intracellular |
T1420 |
3304-3310 |
NNS |
denotes |
levels |
T1421 |
3299-3303 |
NN |
denotes |
Ca2+ |
T1422 |
3315-3317 |
, |
denotes |
, |
T1423 |
3317-3329 |
RB |
denotes |
sequentially |
T1424 |
3344-3346 |
IN |
denotes |
to |
T1425 |
3347-3350 |
DT |
denotes |
the |
T1426 |
3351-3357 |
NN |
denotes |
plasma |
T1427 |
3358-3361 |
CC |
denotes |
and |
T1428 |
3362-3365 |
DT |
denotes |
the |
T1429 |
3374-3382 |
NN |
denotes |
membrane |
T1430 |
3366-3373 |
JJ |
denotes |
nuclear |
T1431 |
3383-3385 |
RB |
denotes |
as |
T1432 |
3391-3393 |
IN |
denotes |
as |
T1433 |
3386-3390 |
RB |
denotes |
well |
T1434 |
3394-3396 |
IN |
denotes |
to |
T1435 |
3397-3410 |
JJ |
denotes |
intracellular |
T1436 |
3411-3419 |
NNS |
denotes |
vesicles |
T1437 |
3419-3420 |
. |
denotes |
. |
T1438 |
3420-3477 |
sentence |
denotes |
Furthermore, annexin A7 associates with lipid rafts [7]. |
T1439 |
3421-3432 |
RB |
denotes |
Furthermore |
T1440 |
3445-3455 |
VBZ |
denotes |
associates |
T1441 |
3432-3434 |
, |
denotes |
, |
T1442 |
3434-3441 |
NN |
denotes |
annexin |
T1443 |
3442-3444 |
NN |
denotes |
A7 |
T1444 |
3456-3460 |
IN |
denotes |
with |
T1445 |
3461-3466 |
NN |
denotes |
lipid |
T1446 |
3467-3472 |
NNS |
denotes |
rafts |
T1447 |
3473-3474 |
-LRB- |
denotes |
[ |
T1448 |
3474-3475 |
CD |
denotes |
7 |
T1449 |
3475-3476 |
-RRB- |
denotes |
] |
T1450 |
3476-3477 |
. |
denotes |
. |
T1451 |
3477-3592 |
sentence |
denotes |
Lipid rafts play a key role in membrane budding and in vesiculation processes such as endo- and exocytosis [8-10]. |
T1452 |
3478-3483 |
NN |
denotes |
Lipid |
T1453 |
3484-3489 |
NNS |
denotes |
rafts |
T1454 |
3490-3494 |
VBP |
denotes |
play |
T1455 |
3495-3496 |
DT |
denotes |
a |
T1456 |
3501-3505 |
NN |
denotes |
role |
T1457 |
3497-3500 |
JJ |
denotes |
key |
T1458 |
3506-3508 |
IN |
denotes |
in |
T1459 |
3509-3517 |
NN |
denotes |
membrane |
T1460 |
3518-3525 |
NN |
denotes |
budding |
T1461 |
3526-3529 |
CC |
denotes |
and |
T1462 |
3530-3532 |
IN |
denotes |
in |
T1463 |
3533-3545 |
NN |
denotes |
vesiculation |
T1464 |
3546-3555 |
NNS |
denotes |
processes |
T1465 |
3556-3560 |
JJ |
denotes |
such |
T1466 |
3561-3563 |
IN |
denotes |
as |
T1467 |
3564-3568 |
AFX |
denotes |
endo |
T1468 |
3574-3584 |
NN |
denotes |
exocytosis |
T1469 |
3568-3569 |
HYPH |
denotes |
- |
T1470 |
3570-3573 |
CC |
denotes |
and |
T1471 |
3585-3586 |
-LRB- |
denotes |
[ |
T1472 |
3586-3587 |
CD |
denotes |
8 |
T1473 |
3587-3588 |
SYM |
denotes |
- |
T1474 |
3588-3590 |
CD |
denotes |
10 |
T1475 |
3590-3591 |
-RRB- |
denotes |
] |
T1476 |
3591-3592 |
. |
denotes |
. |
T1477 |
3592-3680 |
sentence |
denotes |
Two binding partners of annexin A7 have been identified, sorcin and galectin-3 [11-13]. |
T1478 |
3593-3596 |
CD |
denotes |
Two |
T1479 |
3605-3613 |
NNS |
denotes |
partners |
T1480 |
3597-3604 |
VBG |
denotes |
binding |
T1481 |
3638-3648 |
VBN |
denotes |
identified |
T1482 |
3614-3616 |
IN |
denotes |
of |
T1483 |
3617-3624 |
NN |
denotes |
annexin |
T1484 |
3625-3627 |
NN |
denotes |
A7 |
T1485 |
3628-3632 |
VBP |
denotes |
have |
T1486 |
3633-3637 |
VBN |
denotes |
been |
T1487 |
3648-3650 |
, |
denotes |
, |
T1488 |
3650-3656 |
NN |
denotes |
sorcin |
T1489 |
3657-3660 |
CC |
denotes |
and |
T1490 |
3661-3669 |
NN |
denotes |
galectin |
T1491 |
3669-3670 |
HYPH |
denotes |
- |
T1492 |
3670-3671 |
CD |
denotes |
3 |
T1493 |
3672-3673 |
-LRB- |
denotes |
[ |
T1494 |
3673-3675 |
CD |
denotes |
11 |
T1495 |
3675-3676 |
SYM |
denotes |
- |
T1496 |
3676-3678 |
CD |
denotes |
13 |
T1497 |
3678-3679 |
-RRB- |
denotes |
] |
T1498 |
3679-3680 |
. |
denotes |
. |
T1499 |
3680-3767 |
sentence |
denotes |
Sorcin is a Ca2+-binding protein and belongs to the penta EF-hand protein family [14]. |
T1500 |
3681-3687 |
NN |
denotes |
Sorcin |
T1501 |
3688-3690 |
VBZ |
denotes |
is |
T1502 |
3691-3692 |
DT |
denotes |
a |
T1503 |
3706-3713 |
NN |
denotes |
protein |
T1504 |
3693-3697 |
NN |
denotes |
Ca2+ |
T1505 |
3698-3705 |
VBG |
denotes |
binding |
T1506 |
3697-3698 |
HYPH |
denotes |
- |
T1507 |
3714-3717 |
CC |
denotes |
and |
T1508 |
3718-3725 |
VBZ |
denotes |
belongs |
T1509 |
3726-3728 |
IN |
denotes |
to |
T1510 |
3729-3732 |
DT |
denotes |
the |
T1511 |
3755-3761 |
NN |
denotes |
family |
T1512 |
3733-3738 |
NN |
denotes |
penta |
T1513 |
3747-3754 |
NN |
denotes |
protein |
T1514 |
3739-3741 |
NN |
denotes |
EF |
T1515 |
3742-3746 |
NN |
denotes |
hand |
T1516 |
3741-3742 |
HYPH |
denotes |
- |
T1517 |
3762-3763 |
-LRB- |
denotes |
[ |
T1518 |
3763-3765 |
CD |
denotes |
14 |
T1519 |
3765-3766 |
-RRB- |
denotes |
] |
T1520 |
3766-3767 |
. |
denotes |
. |
T1521 |
3767-3833 |
sentence |
denotes |
Like annexin A7 it binds to membranes in a Ca2+-dependent manner. |
T1522 |
3768-3772 |
IN |
denotes |
Like |
T1523 |
3787-3792 |
VBZ |
denotes |
binds |
T1524 |
3773-3780 |
NN |
denotes |
annexin |
T1525 |
3781-3783 |
NN |
denotes |
A7 |
T1526 |
3784-3786 |
PRP |
denotes |
it |
T1527 |
3793-3795 |
IN |
denotes |
to |
T1528 |
3796-3805 |
NNS |
denotes |
membranes |
T1529 |
3806-3808 |
IN |
denotes |
in |
T1530 |
3809-3810 |
DT |
denotes |
a |
T1531 |
3826-3832 |
NN |
denotes |
manner |
T1532 |
3811-3815 |
NN |
denotes |
Ca2+ |
T1533 |
3816-3825 |
JJ |
denotes |
dependent |
T1534 |
3815-3816 |
HYPH |
denotes |
- |
T1535 |
3832-3833 |
. |
denotes |
. |
T1536 |
3833-3957 |
sentence |
denotes |
Sorcin also has been described as interaction partner of the ryanodine receptor, and appears to modulate its function [15]. |
T1537 |
3834-3840 |
NN |
denotes |
Sorcin |
T1538 |
3855-3864 |
VBN |
denotes |
described |
T1539 |
3841-3845 |
RB |
denotes |
also |
T1540 |
3846-3849 |
VBZ |
denotes |
has |
T1541 |
3850-3854 |
VBN |
denotes |
been |
T1542 |
3865-3867 |
IN |
denotes |
as |
T1543 |
3868-3879 |
NN |
denotes |
interaction |
T1544 |
3880-3887 |
NN |
denotes |
partner |
T1545 |
3888-3890 |
IN |
denotes |
of |
T1546 |
3891-3894 |
DT |
denotes |
the |
T1547 |
3905-3913 |
NN |
denotes |
receptor |
T1548 |
3895-3904 |
NN |
denotes |
ryanodine |
T1549 |
3913-3915 |
, |
denotes |
, |
T1550 |
3915-3918 |
CC |
denotes |
and |
T1551 |
3919-3926 |
VBZ |
denotes |
appears |
T1552 |
3927-3929 |
TO |
denotes |
to |
T1553 |
3930-3938 |
VB |
denotes |
modulate |
T1554 |
3939-3942 |
PRP$ |
denotes |
its |
T1555 |
3943-3951 |
NN |
denotes |
function |
T1556 |
3952-3953 |
-LRB- |
denotes |
[ |
T1557 |
3953-3955 |
CD |
denotes |
15 |
T1558 |
3955-3956 |
-RRB- |
denotes |
] |
T1559 |
3956-3957 |
. |
denotes |
. |
T1560 |
3957-4046 |
sentence |
denotes |
The influence of the sorcin/annexin A7 interaction on the ryanodine receptor is unknown. |
T1561 |
3958-3961 |
DT |
denotes |
The |
T1562 |
3962-3971 |
NN |
denotes |
influence |
T1563 |
4035-4037 |
VBZ |
denotes |
is |
T1564 |
3972-3974 |
IN |
denotes |
of |
T1565 |
3975-3978 |
DT |
denotes |
the |
T1566 |
3997-4008 |
NN |
denotes |
interaction |
T1567 |
3979-3985 |
NN |
denotes |
sorcin |
T1568 |
3986-3993 |
NN |
denotes |
annexin |
T1569 |
3985-3986 |
HYPH |
denotes |
/ |
T1570 |
3994-3996 |
NN |
denotes |
A7 |
T1571 |
4009-4011 |
IN |
denotes |
on |
T1572 |
4012-4015 |
DT |
denotes |
the |
T1573 |
4026-4034 |
NN |
denotes |
receptor |
T1574 |
4016-4025 |
NN |
denotes |
ryanodine |
T1575 |
4038-4045 |
JJ |
denotes |
unknown |
T1576 |
4045-4046 |
. |
denotes |
. |
T1577 |
4046-4121 |
sentence |
denotes |
Sorcin and annexin A7 are coexpressed in all tissues examined so far [11]. |
T1578 |
4047-4053 |
NN |
denotes |
Sorcin |
T1579 |
4073-4084 |
VBN |
denotes |
coexpressed |
T1580 |
4054-4057 |
CC |
denotes |
and |
T1581 |
4058-4065 |
NN |
denotes |
annexin |
T1582 |
4066-4068 |
NN |
denotes |
A7 |
T1583 |
4069-4072 |
VBP |
denotes |
are |
T1584 |
4085-4087 |
IN |
denotes |
in |
T1585 |
4088-4091 |
DT |
denotes |
all |
T1586 |
4092-4099 |
NNS |
denotes |
tissues |
T1587 |
4100-4108 |
VBN |
denotes |
examined |
T1588 |
4109-4111 |
RB |
denotes |
so |
T1589 |
4112-4115 |
RB |
denotes |
far |
T1590 |
4116-4117 |
-LRB- |
denotes |
[ |
T1591 |
4117-4119 |
CD |
denotes |
11 |
T1592 |
4119-4120 |
-RRB- |
denotes |
] |
T1593 |
4120-4121 |
. |
denotes |
. |
T1594 |
4121-4222 |
sentence |
denotes |
The binding of annexin A7 and sorcin is Ca2+-dependent and occurs at micromolar Ca2+ concentrations. |
T1595 |
4122-4125 |
DT |
denotes |
The |
T1596 |
4126-4133 |
NN |
denotes |
binding |
T1597 |
4159-4161 |
VBZ |
denotes |
is |
T1598 |
4134-4136 |
IN |
denotes |
of |
T1599 |
4137-4144 |
NN |
denotes |
annexin |
T1600 |
4145-4147 |
NN |
denotes |
A7 |
T1601 |
4148-4151 |
CC |
denotes |
and |
T1602 |
4152-4158 |
NN |
denotes |
sorcin |
T1603 |
4162-4166 |
NN |
denotes |
Ca2+ |
T1604 |
4167-4176 |
JJ |
denotes |
dependent |
T1605 |
4166-4167 |
HYPH |
denotes |
- |
T1606 |
4177-4180 |
CC |
denotes |
and |
T1607 |
4181-4187 |
VBZ |
denotes |
occurs |
T1608 |
4188-4190 |
IN |
denotes |
at |
T1609 |
4191-4201 |
JJ |
denotes |
micromolar |
T1610 |
4202-4206 |
NN |
denotes |
Ca2+ |
T1611 |
4207-4221 |
NNS |
denotes |
concentrations |
T1612 |
4221-4222 |
. |
denotes |
. |
T1613 |
4222-4372 |
sentence |
denotes |
The binding sites have been localised to the amino terminal GGYY and GYGG motifs in sorcin and to the GYPP motif in the amino terminus of annexin A7. |
T1614 |
4223-4226 |
DT |
denotes |
The |
T1615 |
4235-4240 |
NNS |
denotes |
sites |
T1616 |
4227-4234 |
VBG |
denotes |
binding |
T1617 |
4251-4260 |
VBN |
denotes |
localised |
T1618 |
4241-4245 |
VBP |
denotes |
have |
T1619 |
4246-4250 |
VBN |
denotes |
been |
T1620 |
4261-4263 |
IN |
denotes |
to |
T1621 |
4264-4267 |
DT |
denotes |
the |
T1622 |
4297-4303 |
NNS |
denotes |
motifs |
T1623 |
4268-4273 |
NN |
denotes |
amino |
T1624 |
4274-4282 |
JJ |
denotes |
terminal |
T1625 |
4283-4287 |
NN |
denotes |
GGYY |
T1626 |
4288-4291 |
CC |
denotes |
and |
T1627 |
4292-4296 |
NN |
denotes |
GYGG |
T1628 |
4304-4306 |
IN |
denotes |
in |
T1629 |
4307-4313 |
NN |
denotes |
sorcin |
T1630 |
4314-4317 |
CC |
denotes |
and |
T1631 |
4318-4320 |
IN |
denotes |
to |
T1632 |
4321-4324 |
DT |
denotes |
the |
T1633 |
4330-4335 |
NN |
denotes |
motif |
T1634 |
4325-4329 |
NN |
denotes |
GYPP |
T1635 |
4336-4338 |
IN |
denotes |
in |
T1636 |
4339-4342 |
DT |
denotes |
the |
T1637 |
4349-4357 |
NN |
denotes |
terminus |
T1638 |
4343-4348 |
NN |
denotes |
amino |
T1639 |
4358-4360 |
IN |
denotes |
of |
T1640 |
4361-4368 |
NN |
denotes |
annexin |
T1641 |
4369-4371 |
NN |
denotes |
A7 |
T1642 |
4371-4372 |
. |
denotes |
. |
T1643 |
4372-4479 |
sentence |
denotes |
The proteins bind to each other with a stoichiometry of two sorcin molecules per annexin A7 molecule [12]. |
T1644 |
4373-4376 |
DT |
denotes |
The |
T1645 |
4377-4385 |
NN |
denotes |
proteins |
T1646 |
4386-4390 |
VBP |
denotes |
bind |
T1647 |
4391-4393 |
IN |
denotes |
to |
T1648 |
4394-4398 |
DT |
denotes |
each |
T1649 |
4399-4404 |
JJ |
denotes |
other |
T1650 |
4405-4409 |
IN |
denotes |
with |
T1651 |
4410-4411 |
DT |
denotes |
a |
T1652 |
4412-4425 |
NN |
denotes |
stoichiometry |
T1653 |
4426-4428 |
IN |
denotes |
of |
T1654 |
4429-4432 |
CD |
denotes |
two |
T1655 |
4440-4449 |
NNS |
denotes |
molecules |
T1656 |
4433-4439 |
NN |
denotes |
sorcin |
T1657 |
4450-4453 |
IN |
denotes |
per |
T1658 |
4454-4461 |
NN |
denotes |
annexin |
T1659 |
4462-4464 |
NN |
denotes |
A7 |
T1660 |
4465-4473 |
NN |
denotes |
molecule |
T1661 |
4474-4475 |
-LRB- |
denotes |
[ |
T1662 |
4475-4477 |
CD |
denotes |
12 |
T1663 |
4477-4478 |
-RRB- |
denotes |
] |
T1664 |
4478-4479 |
. |
denotes |
. |
T1665 |
4479-4645 |
sentence |
denotes |
Galectin-3 is a multifunctional oncogenic protein with an anti-apoptotic activity found in the extracellular space, in the nucleus and cytoplasm and in mitochondria. |
T1666 |
4480-4488 |
NN |
denotes |
Galectin |
T1667 |
4491-4493 |
VBZ |
denotes |
is |
T1668 |
4488-4489 |
HYPH |
denotes |
- |
T1669 |
4489-4490 |
CD |
denotes |
3 |
T1670 |
4494-4495 |
DT |
denotes |
a |
T1671 |
4522-4529 |
NN |
denotes |
protein |
T1672 |
4496-4511 |
JJ |
denotes |
multifunctional |
T1673 |
4512-4521 |
JJ |
denotes |
oncogenic |
T1674 |
4530-4534 |
IN |
denotes |
with |
T1675 |
4535-4537 |
DT |
denotes |
an |
T1676 |
4553-4561 |
NN |
denotes |
activity |
T1677 |
4538-4552 |
JJ |
denotes |
anti-apoptotic |
T1678 |
4562-4567 |
VBN |
denotes |
found |
T1679 |
4568-4570 |
IN |
denotes |
in |
T1680 |
4571-4574 |
DT |
denotes |
the |
T1681 |
4589-4594 |
NN |
denotes |
space |
T1682 |
4575-4588 |
JJ |
denotes |
extracellular |
T1683 |
4594-4596 |
, |
denotes |
, |
T1684 |
4596-4598 |
IN |
denotes |
in |
T1685 |
4599-4602 |
DT |
denotes |
the |
T1686 |
4603-4610 |
NN |
denotes |
nucleus |
T1687 |
4611-4614 |
CC |
denotes |
and |
T1688 |
4615-4624 |
NN |
denotes |
cytoplasm |
T1689 |
4625-4628 |
CC |
denotes |
and |
T1690 |
4629-4631 |
IN |
denotes |
in |
T1691 |
4632-4644 |
NNS |
denotes |
mitochondria |
T1692 |
4644-4645 |
. |
denotes |
. |
T1693 |
4645-4740 |
sentence |
denotes |
Cytoplasmic galectin-3 correlates with tumor progression and protects mitochondrial integrity. |
T1694 |
4646-4657 |
JJ |
denotes |
Cytoplasmic |
T1695 |
4658-4666 |
NN |
denotes |
galectin |
T1696 |
4669-4679 |
VBZ |
denotes |
correlates |
T1697 |
4666-4667 |
HYPH |
denotes |
- |
T1698 |
4667-4668 |
CD |
denotes |
3 |
T1699 |
4680-4684 |
IN |
denotes |
with |
T1700 |
4685-4690 |
NN |
denotes |
tumor |
T1701 |
4691-4702 |
NN |
denotes |
progression |
T1702 |
4703-4706 |
CC |
denotes |
and |
T1703 |
4707-4715 |
VBZ |
denotes |
protects |
T1704 |
4716-4729 |
JJ |
denotes |
mitochondrial |
T1705 |
4730-4739 |
NN |
denotes |
integrity |
T1706 |
4739-4740 |
. |
denotes |
. |
T1707 |
4740-4868 |
sentence |
denotes |
Down regulation of annexin A7 prevents galectin-3 translocation to the perinuclear membrane and increases galectin-3 secretion. |
T1708 |
4741-4745 |
NN |
denotes |
Down |
T1709 |
4746-4756 |
NN |
denotes |
regulation |
T1710 |
4771-4779 |
VBZ |
denotes |
prevents |
T1711 |
4757-4759 |
IN |
denotes |
of |
T1712 |
4760-4767 |
NN |
denotes |
annexin |
T1713 |
4768-4770 |
NN |
denotes |
A7 |
T1714 |
4780-4788 |
NN |
denotes |
galectin |
T1715 |
4789-4790 |
NN |
denotes |
3 |
T1716 |
4788-4789 |
HYPH |
denotes |
- |
T1717 |
4791-4804 |
NN |
denotes |
translocation |
T1718 |
4805-4807 |
IN |
denotes |
to |
T1719 |
4808-4811 |
DT |
denotes |
the |
T1720 |
4824-4832 |
NN |
denotes |
membrane |
T1721 |
4812-4823 |
JJ |
denotes |
perinuclear |
T1722 |
4833-4836 |
CC |
denotes |
and |
T1723 |
4837-4846 |
VBZ |
denotes |
increases |
T1724 |
4847-4855 |
NN |
denotes |
galectin |
T1725 |
4856-4857 |
NN |
denotes |
3 |
T1726 |
4855-4856 |
HYPH |
denotes |
- |
T1727 |
4858-4867 |
NN |
denotes |
secretion |
T1728 |
4867-4868 |
. |
denotes |
. |
T1729 |
4868-4987 |
sentence |
denotes |
For annexin A7 a role for galectin-3 trafficking, apoptosis regulation, and mitochondrial integrity was proposed [13]. |
T1730 |
4869-4872 |
IN |
denotes |
For |
T1731 |
4973-4981 |
VBN |
denotes |
proposed |
T1732 |
4873-4880 |
NN |
denotes |
annexin |
T1733 |
4881-4883 |
NN |
denotes |
A7 |
T1734 |
4884-4885 |
DT |
denotes |
a |
T1735 |
4886-4890 |
NN |
denotes |
role |
T1736 |
4891-4894 |
IN |
denotes |
for |
T1737 |
4895-4903 |
NN |
denotes |
galectin |
T1738 |
4904-4905 |
NN |
denotes |
3 |
T1739 |
4903-4904 |
HYPH |
denotes |
- |
T1740 |
4906-4917 |
NN |
denotes |
trafficking |
T1741 |
4917-4919 |
, |
denotes |
, |
T1742 |
4919-4928 |
NN |
denotes |
apoptosis |
T1743 |
4929-4939 |
NN |
denotes |
regulation |
T1744 |
4939-4941 |
, |
denotes |
, |
T1745 |
4941-4944 |
CC |
denotes |
and |
T1746 |
4945-4958 |
JJ |
denotes |
mitochondrial |
T1747 |
4959-4968 |
NN |
denotes |
integrity |
T1748 |
4969-4972 |
VBD |
denotes |
was |
T1749 |
4982-4983 |
-LRB- |
denotes |
[ |
T1750 |
4983-4985 |
CD |
denotes |
13 |
T1751 |
4985-4986 |
-RRB- |
denotes |
] |
T1752 |
4986-4987 |
. |
denotes |
. |
T1753 |
4987-5043 |
sentence |
denotes |
The cellular role of annexin A7 is not well understood. |
T1754 |
4988-4991 |
DT |
denotes |
The |
T1755 |
5001-5005 |
NN |
denotes |
role |
T1756 |
4992-5000 |
JJ |
denotes |
cellular |
T1757 |
5032-5042 |
VBN |
denotes |
understood |
T1758 |
5006-5008 |
IN |
denotes |
of |
T1759 |
5009-5016 |
NN |
denotes |
annexin |
T1760 |
5017-5019 |
NN |
denotes |
A7 |
T1761 |
5020-5022 |
VBZ |
denotes |
is |
T1762 |
5023-5026 |
RB |
denotes |
not |
T1763 |
5027-5031 |
RB |
denotes |
well |
T1764 |
5042-5043 |
. |
denotes |
. |
T1765 |
5043-5178 |
sentence |
denotes |
It is thought to regulate and stabilize membrane domains and to have a role in Ca2+ homeostasis and Ca2+-dependent signaling pathways. |
T1766 |
5044-5046 |
PRP |
denotes |
It |
T1767 |
5050-5057 |
VBN |
denotes |
thought |
T1768 |
5047-5049 |
VBZ |
denotes |
is |
T1769 |
5058-5060 |
TO |
denotes |
to |
T1770 |
5061-5069 |
VB |
denotes |
regulate |
T1771 |
5070-5073 |
CC |
denotes |
and |
T1772 |
5074-5083 |
VB |
denotes |
stabilize |
T1773 |
5084-5092 |
NN |
denotes |
membrane |
T1774 |
5093-5100 |
NNS |
denotes |
domains |
T1775 |
5101-5104 |
CC |
denotes |
and |
T1776 |
5105-5107 |
TO |
denotes |
to |
T1777 |
5108-5112 |
VB |
denotes |
have |
T1778 |
5113-5114 |
DT |
denotes |
a |
T1779 |
5115-5119 |
NN |
denotes |
role |
T1780 |
5120-5122 |
IN |
denotes |
in |
T1781 |
5123-5127 |
NN |
denotes |
Ca2+ |
T1782 |
5128-5139 |
NN |
denotes |
homeostasis |
T1783 |
5140-5143 |
CC |
denotes |
and |
T1784 |
5144-5148 |
NN |
denotes |
Ca2+ |
T1785 |
5149-5158 |
JJ |
denotes |
dependent |
T1786 |
5148-5149 |
HYPH |
denotes |
- |
T1787 |
5169-5177 |
NNS |
denotes |
pathways |
T1788 |
5159-5168 |
NN |
denotes |
signaling |
T1789 |
5177-5178 |
. |
denotes |
. |
T1790 |
5178-5278 |
sentence |
denotes |
These proposals are supported by data obtained from analysis of annexin A7 deficient mouse mutants. |
T1791 |
5179-5184 |
DT |
denotes |
These |
T1792 |
5185-5194 |
NNS |
denotes |
proposals |
T1793 |
5199-5208 |
VBN |
denotes |
supported |
T1794 |
5195-5198 |
VBP |
denotes |
are |
T1795 |
5209-5211 |
IN |
denotes |
by |
T1796 |
5212-5216 |
NNS |
denotes |
data |
T1797 |
5217-5225 |
VBN |
denotes |
obtained |
T1798 |
5226-5230 |
IN |
denotes |
from |
T1799 |
5231-5239 |
NN |
denotes |
analysis |
T1800 |
5240-5242 |
IN |
denotes |
of |
T1801 |
5243-5250 |
NN |
denotes |
annexin |
T1802 |
5251-5253 |
NN |
denotes |
A7 |
T1803 |
5254-5263 |
JJ |
denotes |
deficient |
T1804 |
5270-5277 |
NNS |
denotes |
mutants |
T1805 |
5264-5269 |
NN |
denotes |
mouse |
T1806 |
5277-5278 |
. |
denotes |
. |
T1807 |
5278-5374 |
sentence |
denotes |
Two annexin A7 129Sv null mice strains were generated independently using a different strategy. |
T1808 |
5279-5282 |
CD |
denotes |
Two |
T1809 |
5310-5317 |
NNS |
denotes |
strains |
T1810 |
5283-5290 |
NN |
denotes |
annexin |
T1811 |
5291-5293 |
NN |
denotes |
A7 |
T1812 |
5294-5299 |
NN |
denotes |
129Sv |
T1813 |
5300-5304 |
JJ |
denotes |
null |
T1814 |
5305-5309 |
NNS |
denotes |
mice |
T1815 |
5323-5332 |
VBN |
denotes |
generated |
T1816 |
5318-5322 |
VBD |
denotes |
were |
T1817 |
5333-5346 |
RB |
denotes |
independently |
T1818 |
5347-5352 |
VBG |
denotes |
using |
T1819 |
5353-5354 |
DT |
denotes |
a |
T1820 |
5365-5373 |
NN |
denotes |
strategy |
T1821 |
5355-5364 |
JJ |
denotes |
different |
T1822 |
5373-5374 |
. |
denotes |
. |
T1823 |
5374-5419 |
sentence |
denotes |
The one of Srivastava et al. [16] is lethal. |
T1824 |
5375-5378 |
DT |
denotes |
The |
T1825 |
5379-5382 |
CD |
denotes |
one |
T1826 |
5409-5411 |
VBZ |
denotes |
is |
T1827 |
5383-5385 |
IN |
denotes |
of |
T1828 |
5386-5396 |
NNP |
denotes |
Srivastava |
T1829 |
5397-5399 |
FW |
denotes |
et |
T1830 |
5400-5403 |
FW |
denotes |
al. |
T1831 |
5404-5405 |
-LRB- |
denotes |
[ |
T1832 |
5405-5407 |
CD |
denotes |
16 |
T1833 |
5407-5408 |
-RRB- |
denotes |
] |
T1834 |
5412-5418 |
JJ |
denotes |
lethal |
T1835 |
5418-5419 |
. |
denotes |
. |
T1836 |
5419-5495 |
sentence |
denotes |
Heterozygous mice exhibit an insulin secretion defect and tumor phenotypes. |
T1837 |
5420-5432 |
JJ |
denotes |
Heterozygous |
T1838 |
5433-5437 |
NNS |
denotes |
mice |
T1839 |
5438-5445 |
VBP |
denotes |
exhibit |
T1840 |
5446-5448 |
DT |
denotes |
an |
T1841 |
5467-5473 |
NN |
denotes |
defect |
T1842 |
5449-5456 |
NN |
denotes |
insulin |
T1843 |
5457-5466 |
NN |
denotes |
secretion |
T1844 |
5474-5477 |
CC |
denotes |
and |
T1845 |
5478-5483 |
NN |
denotes |
tumor |
T1846 |
5484-5494 |
NNS |
denotes |
phenotypes |
T1847 |
5494-5495 |
. |
denotes |
. |
T1848 |
5495-5624 |
sentence |
denotes |
The null strain reported by Herr et al. [17] is viable, healthy, and shows no insulin secretion defect or other obvious defects. |
T1849 |
5496-5499 |
DT |
denotes |
The |
T1850 |
5505-5511 |
NN |
denotes |
strain |
T1851 |
5500-5504 |
JJ |
denotes |
null |
T1852 |
5541-5543 |
VBZ |
denotes |
is |
T1853 |
5512-5520 |
VBN |
denotes |
reported |
T1854 |
5521-5523 |
IN |
denotes |
by |
T1855 |
5524-5528 |
NNP |
denotes |
Herr |
T1856 |
5529-5531 |
FW |
denotes |
et |
T1857 |
5532-5535 |
FW |
denotes |
al. |
T1858 |
5536-5537 |
-LRB- |
denotes |
[ |
T1859 |
5537-5539 |
CD |
denotes |
17 |
T1860 |
5539-5540 |
-RRB- |
denotes |
] |
T1861 |
5544-5550 |
JJ |
denotes |
viable |
T1862 |
5552-5559 |
JJ |
denotes |
healthy |
T1863 |
5550-5552 |
, |
denotes |
, |
T1864 |
5559-5561 |
, |
denotes |
, |
T1865 |
5561-5564 |
CC |
denotes |
and |
T1866 |
5565-5570 |
VBZ |
denotes |
shows |
T1867 |
5571-5573 |
DT |
denotes |
no |
T1868 |
5592-5598 |
NN |
denotes |
defect |
T1869 |
5574-5581 |
NN |
denotes |
insulin |
T1870 |
5582-5591 |
NN |
denotes |
secretion |
T1871 |
5599-5601 |
CC |
denotes |
or |
T1872 |
5602-5607 |
JJ |
denotes |
other |
T1873 |
5616-5623 |
NNS |
denotes |
defects |
T1874 |
5608-5615 |
JJ |
denotes |
obvious |
T1875 |
5623-5624 |
. |
denotes |
. |
T1876 |
5624-5707 |
sentence |
denotes |
However, in isolated cardiomyocytes the frequency-induced shortening is disturbed. |
T1877 |
5625-5632 |
RB |
denotes |
However |
T1878 |
5697-5706 |
VBN |
denotes |
disturbed |
T1879 |
5632-5634 |
, |
denotes |
, |
T1880 |
5634-5636 |
IN |
denotes |
in |
T1881 |
5637-5645 |
VBN |
denotes |
isolated |
T1882 |
5646-5660 |
NNS |
denotes |
cardiomyocytes |
T1883 |
5661-5664 |
DT |
denotes |
the |
T1884 |
5683-5693 |
NN |
denotes |
shortening |
T1885 |
5665-5674 |
NN |
denotes |
frequency |
T1886 |
5675-5682 |
VBN |
denotes |
induced |
T1887 |
5674-5675 |
HYPH |
denotes |
- |
T1888 |
5694-5696 |
VBZ |
denotes |
is |
T1889 |
5706-5707 |
. |
denotes |
. |
T1890 |
5707-5801 |
sentence |
denotes |
Here we have focused on the analysis of red blood cells and platelets of the anxA7-/- mutant. |
T1891 |
5708-5712 |
RB |
denotes |
Here |
T1892 |
5721-5728 |
VBN |
denotes |
focused |
T1893 |
5713-5715 |
PRP |
denotes |
we |
T1894 |
5716-5720 |
VBP |
denotes |
have |
T1895 |
5729-5731 |
IN |
denotes |
on |
T1896 |
5732-5735 |
DT |
denotes |
the |
T1897 |
5736-5744 |
NN |
denotes |
analysis |
T1898 |
5745-5747 |
IN |
denotes |
of |
T1899 |
5748-5751 |
JJ |
denotes |
red |
T1900 |
5758-5763 |
NNS |
denotes |
cells |
T1901 |
5752-5757 |
NN |
denotes |
blood |
T1902 |
5764-5767 |
CC |
denotes |
and |
T1903 |
5768-5777 |
NNS |
denotes |
platelets |
T1904 |
5778-5780 |
IN |
denotes |
of |
T1905 |
5781-5784 |
DT |
denotes |
the |
T1906 |
5794-5800 |
NN |
denotes |
mutant |
T1907 |
5785-5790 |
JJ |
denotes |
anxA7 |
T1908 |
5790-5791 |
SYM |
denotes |
- |
T1909 |
5791-5792 |
HYPH |
denotes |
/ |
T1910 |
5792-5793 |
SYM |
denotes |
- |
T1911 |
5800-5801 |
. |
denotes |
. |
T1912 |
5801-5973 |
sentence |
denotes |
Generally, red blood cells and platelets were thought not to contain annexin A7 and only recently the 47 kDa isoform has been reported as component of red blood cells [7]. |
T1913 |
5802-5811 |
RB |
denotes |
Generally |
T1914 |
5848-5855 |
VBN |
denotes |
thought |
T1915 |
5811-5813 |
, |
denotes |
, |
T1916 |
5813-5816 |
JJ |
denotes |
red |
T1917 |
5823-5828 |
NNS |
denotes |
cells |
T1918 |
5817-5822 |
NN |
denotes |
blood |
T1919 |
5829-5832 |
CC |
denotes |
and |
T1920 |
5833-5842 |
NNS |
denotes |
platelets |
T1921 |
5843-5847 |
VBD |
denotes |
were |
T1922 |
5856-5859 |
RB |
denotes |
not |
T1923 |
5860-5862 |
TO |
denotes |
to |
T1924 |
5863-5870 |
VB |
denotes |
contain |
T1925 |
5871-5878 |
NN |
denotes |
annexin |
T1926 |
5879-5881 |
NN |
denotes |
A7 |
T1927 |
5882-5885 |
CC |
denotes |
and |
T1928 |
5886-5890 |
RB |
denotes |
only |
T1929 |
5891-5899 |
RB |
denotes |
recently |
T1930 |
5928-5936 |
VBN |
denotes |
reported |
T1931 |
5900-5903 |
DT |
denotes |
the |
T1932 |
5911-5918 |
NN |
denotes |
isoform |
T1933 |
5904-5906 |
CD |
denotes |
47 |
T1934 |
5907-5910 |
NN |
denotes |
kDa |
T1935 |
5919-5922 |
VBZ |
denotes |
has |
T1936 |
5923-5927 |
VBN |
denotes |
been |
T1937 |
5937-5939 |
IN |
denotes |
as |
T1938 |
5940-5949 |
NN |
denotes |
component |
T1939 |
5950-5952 |
IN |
denotes |
of |
T1940 |
5953-5956 |
JJ |
denotes |
red |
T1941 |
5963-5968 |
NNS |
denotes |
cells |
T1942 |
5957-5962 |
NN |
denotes |
blood |
T1943 |
5969-5970 |
-LRB- |
denotes |
[ |
T1944 |
5970-5971 |
CD |
denotes |
7 |
T1945 |
5971-5972 |
-RRB- |
denotes |
] |
T1946 |
5972-5973 |
. |
denotes |
. |
T1947 |
5973-6094 |
sentence |
denotes |
Red blood cells undergo various biochemical or morphological changes that appear to be Ca2+-dependent processes [18,19]. |
T1948 |
5974-5977 |
JJ |
denotes |
Red |
T1949 |
5984-5989 |
NNS |
denotes |
cells |
T1950 |
5978-5983 |
NN |
denotes |
blood |
T1951 |
5990-5997 |
VBP |
denotes |
undergo |
T1952 |
5998-6005 |
JJ |
denotes |
various |
T1953 |
6035-6042 |
NNS |
denotes |
changes |
T1954 |
6006-6017 |
JJ |
denotes |
biochemical |
T1955 |
6018-6020 |
CC |
denotes |
or |
T1956 |
6021-6034 |
JJ |
denotes |
morphological |
T1957 |
6043-6047 |
WDT |
denotes |
that |
T1958 |
6048-6054 |
VBP |
denotes |
appear |
T1959 |
6055-6057 |
TO |
denotes |
to |
T1960 |
6058-6060 |
VB |
denotes |
be |
T1961 |
6061-6065 |
NN |
denotes |
Ca2+ |
T1962 |
6066-6075 |
JJ |
denotes |
dependent |
T1963 |
6065-6066 |
HYPH |
denotes |
- |
T1964 |
6076-6085 |
NNS |
denotes |
processes |
T1965 |
6086-6087 |
-LRB- |
denotes |
[ |
T1966 |
6090-6092 |
CD |
denotes |
19 |
T1967 |
6087-6089 |
CD |
denotes |
18 |
T1968 |
6089-6090 |
, |
denotes |
, |
T1969 |
6092-6093 |
-RRB- |
denotes |
] |
T1970 |
6093-6094 |
. |
denotes |
. |
T1971 |
6094-6204 |
sentence |
denotes |
One of them is the release of hemoglobin-containing exovesicles occurring in vivo as well as in vitro [7,20]. |
T1972 |
6095-6098 |
CD |
denotes |
One |
T1973 |
6107-6109 |
VBZ |
denotes |
is |
T1974 |
6099-6101 |
IN |
denotes |
of |
T1975 |
6102-6106 |
PRP |
denotes |
them |
T1976 |
6110-6113 |
DT |
denotes |
the |
T1977 |
6114-6121 |
NN |
denotes |
release |
T1978 |
6122-6124 |
IN |
denotes |
of |
T1979 |
6125-6135 |
NN |
denotes |
hemoglobin |
T1980 |
6136-6146 |
VBG |
denotes |
containing |
T1981 |
6135-6136 |
HYPH |
denotes |
- |
T1982 |
6147-6158 |
NNS |
denotes |
exovesicles |
T1983 |
6159-6168 |
VBG |
denotes |
occurring |
T1984 |
6169-6171 |
FW |
denotes |
in |
T1985 |
6172-6176 |
FW |
denotes |
vivo |
T1986 |
6177-6179 |
RB |
denotes |
as |
T1987 |
6185-6187 |
IN |
denotes |
as |
T1988 |
6180-6184 |
RB |
denotes |
well |
T1989 |
6188-6190 |
FW |
denotes |
in |
T1990 |
6191-6196 |
FW |
denotes |
vitro |
T1991 |
6197-6198 |
-LRB- |
denotes |
[ |
T1992 |
6200-6202 |
CD |
denotes |
20 |
T1993 |
6198-6199 |
CD |
denotes |
7 |
T1994 |
6199-6200 |
, |
denotes |
, |
T1995 |
6202-6203 |
-RRB- |
denotes |
] |
T1996 |
6203-6204 |
. |
denotes |
. |
T1997 |
6204-6339 |
sentence |
denotes |
This process is thought to represent a protective method of the red blood cell against an attack by for example complement components. |
T1998 |
6205-6209 |
DT |
denotes |
This |
T1999 |
6210-6217 |
NN |
denotes |
process |
T2000 |
6221-6228 |
VBN |
denotes |
thought |
T2001 |
6218-6220 |
VBZ |
denotes |
is |
T2002 |
6229-6231 |
TO |
denotes |
to |
T2003 |
6232-6241 |
VB |
denotes |
represent |
T2004 |
6242-6243 |
DT |
denotes |
a |
T2005 |
6255-6261 |
NN |
denotes |
method |
T2006 |
6244-6254 |
JJ |
denotes |
protective |
T2007 |
6262-6264 |
IN |
denotes |
of |
T2008 |
6265-6268 |
DT |
denotes |
the |
T2009 |
6279-6283 |
NN |
denotes |
cell |
T2010 |
6269-6272 |
JJ |
denotes |
red |
T2011 |
6273-6278 |
NN |
denotes |
blood |
T2012 |
6284-6291 |
IN |
denotes |
against |
T2013 |
6292-6294 |
DT |
denotes |
an |
T2014 |
6295-6301 |
NN |
denotes |
attack |
T2015 |
6302-6304 |
IN |
denotes |
by |
T2016 |
6305-6308 |
IN |
denotes |
for |
T2017 |
6328-6338 |
NNS |
denotes |
components |
T2018 |
6309-6316 |
NN |
denotes |
example |
T2019 |
6317-6327 |
NN |
denotes |
complement |
T2020 |
6338-6339 |
. |
denotes |
. |
T2021 |
6339-6466 |
sentence |
denotes |
Red blood cells which lack the ability to vesiculate cause a disease with red blood cell destruction and haemoglobinuria [21]. |
T2022 |
6340-6343 |
JJ |
denotes |
Red |
T2023 |
6350-6355 |
NNS |
denotes |
cells |
T2024 |
6344-6349 |
NN |
denotes |
blood |
T2025 |
6393-6398 |
VB |
denotes |
cause |
T2026 |
6356-6361 |
WDT |
denotes |
which |
T2027 |
6362-6366 |
VBP |
denotes |
lack |
T2028 |
6367-6370 |
DT |
denotes |
the |
T2029 |
6371-6378 |
NN |
denotes |
ability |
T2030 |
6379-6381 |
TO |
denotes |
to |
T2031 |
6382-6392 |
VB |
denotes |
vesiculate |
T2032 |
6399-6400 |
DT |
denotes |
a |
T2033 |
6401-6408 |
NN |
denotes |
disease |
T2034 |
6409-6413 |
IN |
denotes |
with |
T2035 |
6414-6417 |
JJ |
denotes |
red |
T2036 |
6424-6428 |
NN |
denotes |
cell |
T2037 |
6418-6423 |
NN |
denotes |
blood |
T2038 |
6429-6440 |
NN |
denotes |
destruction |
T2039 |
6441-6444 |
CC |
denotes |
and |
T2040 |
6445-6460 |
NN |
denotes |
haemoglobinuria |
T2041 |
6461-6462 |
-LRB- |
denotes |
[ |
T2042 |
6462-6464 |
CD |
denotes |
21 |
T2043 |
6464-6465 |
-RRB- |
denotes |
] |
T2044 |
6465-6466 |
. |
denotes |
. |
T2045 |
6466-6572 |
sentence |
denotes |
There exist two types of vesicles, micro- and nanovesicles with a size of 180 nm and 60 nm, respectively. |
T2046 |
6467-6472 |
EX |
denotes |
There |
T2047 |
6473-6478 |
VBP |
denotes |
exist |
T2048 |
6479-6482 |
CD |
denotes |
two |
T2049 |
6483-6488 |
NNS |
denotes |
types |
T2050 |
6489-6491 |
IN |
denotes |
of |
T2051 |
6492-6500 |
NNS |
denotes |
vesicles |
T2052 |
6500-6502 |
, |
denotes |
, |
T2053 |
6502-6507 |
AFX |
denotes |
micro |
T2054 |
6513-6525 |
NNS |
denotes |
nanovesicles |
T2055 |
6507-6508 |
HYPH |
denotes |
- |
T2056 |
6509-6512 |
CC |
denotes |
and |
T2057 |
6526-6530 |
IN |
denotes |
with |
T2058 |
6531-6532 |
DT |
denotes |
a |
T2059 |
6533-6537 |
NN |
denotes |
size |
T2060 |
6538-6540 |
IN |
denotes |
of |
T2061 |
6541-6544 |
CD |
denotes |
180 |
T2062 |
6545-6547 |
NN |
denotes |
nm |
T2063 |
6548-6551 |
CC |
denotes |
and |
T2064 |
6552-6554 |
CD |
denotes |
60 |
T2065 |
6555-6557 |
NN |
denotes |
nm |
T2066 |
6557-6559 |
, |
denotes |
, |
T2067 |
6559-6571 |
RB |
denotes |
respectively |
T2068 |
6571-6572 |
. |
denotes |
. |
T2069 |
6572-6858 |
sentence |
denotes |
They are enriched in cholesterol and sphingolipid rich lipid raft domains that are associated with proteins like acetylcholinesterase, cell surface proteins including a complement receptor, and the lipid raft proteins stomatin and flotillin, but they lack any cytoskeletal protein [7]. |
T2070 |
6573-6577 |
PRP |
denotes |
They |
T2071 |
6582-6590 |
VBN |
denotes |
enriched |
T2072 |
6578-6581 |
VBP |
denotes |
are |
T2073 |
6591-6593 |
IN |
denotes |
in |
T2074 |
6594-6605 |
NN |
denotes |
cholesterol |
T2075 |
6623-6627 |
JJ |
denotes |
rich |
T2076 |
6606-6609 |
CC |
denotes |
and |
T2077 |
6610-6622 |
NN |
denotes |
sphingolipid |
T2078 |
6639-6646 |
NNS |
denotes |
domains |
T2079 |
6628-6633 |
NN |
denotes |
lipid |
T2080 |
6634-6638 |
NN |
denotes |
raft |
T2081 |
6647-6651 |
WDT |
denotes |
that |
T2082 |
6656-6666 |
VBN |
denotes |
associated |
T2083 |
6652-6655 |
VBP |
denotes |
are |
T2084 |
6667-6671 |
IN |
denotes |
with |
T2085 |
6672-6680 |
NN |
denotes |
proteins |
T2086 |
6681-6685 |
IN |
denotes |
like |
T2087 |
6686-6706 |
NN |
denotes |
acetylcholinesterase |
T2088 |
6706-6708 |
, |
denotes |
, |
T2089 |
6708-6712 |
NN |
denotes |
cell |
T2090 |
6721-6729 |
NN |
denotes |
proteins |
T2091 |
6713-6720 |
NN |
denotes |
surface |
T2092 |
6730-6739 |
VBG |
denotes |
including |
T2093 |
6740-6741 |
DT |
denotes |
a |
T2094 |
6753-6761 |
NN |
denotes |
receptor |
T2095 |
6742-6752 |
NN |
denotes |
complement |
T2096 |
6761-6763 |
, |
denotes |
, |
T2097 |
6763-6766 |
CC |
denotes |
and |
T2098 |
6767-6770 |
DT |
denotes |
the |
T2099 |
6782-6790 |
NN |
denotes |
proteins |
T2100 |
6771-6776 |
NN |
denotes |
lipid |
T2101 |
6777-6781 |
NN |
denotes |
raft |
T2102 |
6791-6799 |
NN |
denotes |
stomatin |
T2103 |
6800-6803 |
CC |
denotes |
and |
T2104 |
6804-6813 |
NN |
denotes |
flotillin |
T2105 |
6813-6815 |
, |
denotes |
, |
T2106 |
6815-6818 |
CC |
denotes |
but |
T2107 |
6819-6823 |
PRP |
denotes |
they |
T2108 |
6824-6828 |
VBP |
denotes |
lack |
T2109 |
6829-6832 |
DT |
denotes |
any |
T2110 |
6846-6853 |
NN |
denotes |
protein |
T2111 |
6833-6845 |
JJ |
denotes |
cytoskeletal |
T2112 |
6854-6855 |
-LRB- |
denotes |
[ |
T2113 |
6855-6856 |
CD |
denotes |
7 |
T2114 |
6856-6857 |
-RRB- |
denotes |
] |
T2115 |
6857-6858 |
. |
denotes |
. |
T2116 |
6858-6935 |
sentence |
denotes |
Furthermore, they contain sorcin and annexin A7 attached to the lipid rafts. |
T2117 |
6859-6870 |
RB |
denotes |
Furthermore |
T2118 |
6877-6884 |
VBP |
denotes |
contain |
T2119 |
6870-6872 |
, |
denotes |
, |
T2120 |
6872-6876 |
PRP |
denotes |
they |
T2121 |
6885-6891 |
NN |
denotes |
sorcin |
T2122 |
6892-6895 |
CC |
denotes |
and |
T2123 |
6896-6903 |
NN |
denotes |
annexin |
T2124 |
6904-6906 |
NN |
denotes |
A7 |
T2125 |
6907-6915 |
VBN |
denotes |
attached |
T2126 |
6916-6918 |
IN |
denotes |
to |
T2127 |
6919-6922 |
DT |
denotes |
the |
T2128 |
6929-6934 |
NNS |
denotes |
rafts |
T2129 |
6923-6928 |
NN |
denotes |
lipid |
T2130 |
6934-6935 |
. |
denotes |
. |
T2131 |
6935-6984 |
sentence |
denotes |
Both proteins are more abundant in nanovesicles. |
T2132 |
6936-6940 |
DT |
denotes |
Both |
T2133 |
6941-6949 |
NN |
denotes |
proteins |
T2134 |
6950-6953 |
VBP |
denotes |
are |
T2135 |
6954-6958 |
RBR |
denotes |
more |
T2136 |
6959-6967 |
JJ |
denotes |
abundant |
T2137 |
6968-6970 |
IN |
denotes |
in |
T2138 |
6971-6983 |
NNS |
denotes |
nanovesicles |
T2139 |
6983-6984 |
. |
denotes |
. |
T2140 |
6984-7169 |
sentence |
denotes |
The vesicle formation goes along with several other changes in the red blood cell like cytoskeletal rearrangements and changes in the phospholipid orientation in the cellular membrane. |
T2141 |
6985-6988 |
DT |
denotes |
The |
T2142 |
6997-7006 |
NN |
denotes |
formation |
T2143 |
6989-6996 |
NN |
denotes |
vesicle |
T2144 |
7007-7011 |
VBZ |
denotes |
goes |
T2145 |
7012-7017 |
RP |
denotes |
along |
T2146 |
7018-7022 |
IN |
denotes |
with |
T2147 |
7023-7030 |
JJ |
denotes |
several |
T2148 |
7037-7044 |
NNS |
denotes |
changes |
T2149 |
7031-7036 |
JJ |
denotes |
other |
T2150 |
7045-7047 |
IN |
denotes |
in |
T2151 |
7048-7051 |
DT |
denotes |
the |
T2152 |
7062-7066 |
NN |
denotes |
cell |
T2153 |
7052-7055 |
JJ |
denotes |
red |
T2154 |
7056-7061 |
NN |
denotes |
blood |
T2155 |
7067-7071 |
IN |
denotes |
like |
T2156 |
7072-7084 |
JJ |
denotes |
cytoskeletal |
T2157 |
7085-7099 |
NNS |
denotes |
rearrangements |
T2158 |
7100-7103 |
CC |
denotes |
and |
T2159 |
7104-7111 |
NNS |
denotes |
changes |
T2160 |
7112-7114 |
IN |
denotes |
in |
T2161 |
7115-7118 |
DT |
denotes |
the |
T2162 |
7132-7143 |
NN |
denotes |
orientation |
T2163 |
7119-7131 |
NN |
denotes |
phospholipid |
T2164 |
7144-7146 |
IN |
denotes |
in |
T2165 |
7147-7150 |
DT |
denotes |
the |
T2166 |
7160-7168 |
NN |
denotes |
membrane |
T2167 |
7151-7159 |
JJ |
denotes |
cellular |
T2168 |
7168-7169 |
. |
denotes |
. |
T2443 |
7180-7187 |
NN |
denotes |
Annexin |
T2444 |
7188-7190 |
NN |
denotes |
A7 |
T2445 |
7191-7201 |
NN |
denotes |
expression |
T2446 |
7202-7204 |
IN |
denotes |
in |
T2447 |
7205-7208 |
JJ |
denotes |
red |
T2448 |
7215-7220 |
NNS |
denotes |
cells |
T2449 |
7209-7214 |
NN |
denotes |
blood |
T2450 |
7220-7222 |
, |
denotes |
, |
T2451 |
7222-7225 |
JJ |
denotes |
red |
T2452 |
7232-7236 |
NN |
denotes |
cell |
T2453 |
7226-7231 |
NN |
denotes |
blood |
T2454 |
7237-7244 |
VBN |
denotes |
derived |
T2455 |
7245-7256 |
NNS |
denotes |
exovesicles |
T2456 |
7257-7260 |
CC |
denotes |
and |
T2457 |
7261-7263 |
IN |
denotes |
in |
T2458 |
7264-7273 |
NNS |
denotes |
platelets |
T2459 |
7273-7514 |
sentence |
denotes |
Salzer et al. recently reported the presence of the 47 kDa isoform of annexin A7 and its partner sorcin in micro- and nanovesicles derived from red blood cells where they are located in the lumen and are also enriched in membrane rafts [7]. |
T2460 |
7274-7280 |
NNP |
denotes |
Salzer |
T2461 |
7297-7305 |
VBD |
denotes |
reported |
T2462 |
7281-7283 |
FW |
denotes |
et |
T2463 |
7284-7287 |
FW |
denotes |
al. |
T2464 |
7288-7296 |
RB |
denotes |
recently |
T2465 |
7306-7309 |
DT |
denotes |
the |
T2466 |
7310-7318 |
NN |
denotes |
presence |
T2467 |
7319-7321 |
IN |
denotes |
of |
T2468 |
7322-7325 |
DT |
denotes |
the |
T2469 |
7333-7340 |
NN |
denotes |
isoform |
T2470 |
7326-7328 |
CD |
denotes |
47 |
T2471 |
7329-7332 |
NN |
denotes |
kDa |
T2472 |
7341-7343 |
IN |
denotes |
of |
T2473 |
7344-7351 |
NN |
denotes |
annexin |
T2474 |
7352-7354 |
NN |
denotes |
A7 |
T2475 |
7355-7358 |
CC |
denotes |
and |
T2476 |
7359-7362 |
PRP$ |
denotes |
its |
T2477 |
7363-7370 |
NN |
denotes |
partner |
T2478 |
7371-7377 |
NN |
denotes |
sorcin |
T2479 |
7378-7380 |
IN |
denotes |
in |
T2480 |
7381-7386 |
NN |
denotes |
micro |
T2481 |
7386-7387 |
HYPH |
denotes |
- |
T2482 |
7388-7391 |
CC |
denotes |
and |
T2483 |
7392-7404 |
NNS |
denotes |
nanovesicles |
T2484 |
7405-7412 |
VBN |
denotes |
derived |
T2485 |
7413-7417 |
IN |
denotes |
from |
T2486 |
7418-7421 |
JJ |
denotes |
red |
T2487 |
7428-7433 |
NNS |
denotes |
cells |
T2488 |
7422-7427 |
NN |
denotes |
blood |
T2489 |
7434-7439 |
WRB |
denotes |
where |
T2490 |
7449-7456 |
VBN |
denotes |
located |
T2491 |
7440-7444 |
PRP |
denotes |
they |
T2492 |
7445-7448 |
VBP |
denotes |
are |
T2493 |
7457-7459 |
IN |
denotes |
in |
T2494 |
7460-7463 |
DT |
denotes |
the |
T2495 |
7464-7469 |
NN |
denotes |
lumen |
T2496 |
7470-7473 |
CC |
denotes |
and |
T2497 |
7474-7477 |
VBP |
denotes |
are |
T2498 |
7483-7491 |
VBN |
denotes |
enriched |
T2499 |
7478-7482 |
RB |
denotes |
also |
T2500 |
7492-7494 |
IN |
denotes |
in |
T2501 |
7495-7503 |
NN |
denotes |
membrane |
T2502 |
7504-7509 |
NNS |
denotes |
rafts |
T2503 |
7510-7511 |
-LRB- |
denotes |
[ |
T2504 |
7511-7512 |
CD |
denotes |
7 |
T2505 |
7512-7513 |
-RRB- |
denotes |
] |
T2506 |
7513-7514 |
. |
denotes |
. |
T2507 |
7514-7608 |
sentence |
denotes |
We have now extended these findings and show here that the 51 kDa isoform is present as well. |
T2508 |
7515-7517 |
PRP |
denotes |
We |
T2509 |
7527-7535 |
VBN |
denotes |
extended |
T2510 |
7518-7522 |
VBP |
denotes |
have |
T2511 |
7523-7526 |
RB |
denotes |
now |
T2512 |
7536-7541 |
DT |
denotes |
these |
T2513 |
7542-7550 |
NNS |
denotes |
findings |
T2514 |
7551-7554 |
CC |
denotes |
and |
T2515 |
7555-7559 |
VBP |
denotes |
show |
T2516 |
7560-7564 |
RB |
denotes |
here |
T2517 |
7565-7569 |
IN |
denotes |
that |
T2518 |
7589-7591 |
VBZ |
denotes |
is |
T2519 |
7570-7573 |
DT |
denotes |
the |
T2520 |
7581-7588 |
NN |
denotes |
isoform |
T2521 |
7574-7576 |
CD |
denotes |
51 |
T2522 |
7577-7580 |
NN |
denotes |
kDa |
T2523 |
7592-7599 |
JJ |
denotes |
present |
T2524 |
7600-7602 |
RB |
denotes |
as |
T2525 |
7603-7607 |
RB |
denotes |
well |
T2526 |
7607-7608 |
. |
denotes |
. |
T2527 |
7608-7719 |
sentence |
denotes |
In western blots of human red blood cells the 47 and 51 kDa isoforms were detected using mAb 203–217 (Fig. 1). |
T2528 |
7609-7611 |
IN |
denotes |
In |
T2529 |
7683-7691 |
VBN |
denotes |
detected |
T2530 |
7612-7619 |
JJ |
denotes |
western |
T2531 |
7620-7625 |
NNS |
denotes |
blots |
T2532 |
7626-7628 |
IN |
denotes |
of |
T2533 |
7629-7634 |
JJ |
denotes |
human |
T2534 |
7645-7650 |
NNS |
denotes |
cells |
T2535 |
7635-7638 |
JJ |
denotes |
red |
T2536 |
7639-7644 |
NN |
denotes |
blood |
T2537 |
7651-7654 |
DT |
denotes |
the |
T2538 |
7669-7677 |
NNS |
denotes |
isoforms |
T2539 |
7655-7657 |
CD |
denotes |
47 |
T2540 |
7665-7668 |
NN |
denotes |
kDa |
T2541 |
7658-7661 |
CC |
denotes |
and |
T2542 |
7662-7664 |
CD |
denotes |
51 |
T2543 |
7678-7682 |
VBD |
denotes |
were |
T2544 |
7692-7697 |
VBG |
denotes |
using |
T2545 |
7698-7701 |
NN |
denotes |
mAb |
T2546 |
7702-7709 |
CD |
denotes |
203–217 |
T2547 |
7710-7711 |
-LRB- |
denotes |
( |
T2548 |
7711-7715 |
NN |
denotes |
Fig. |
T2549 |
7716-7717 |
CD |
denotes |
1 |
T2550 |
7717-7718 |
-RRB- |
denotes |
) |
T2551 |
7718-7719 |
. |
denotes |
. |
T2552 |
7719-7866 |
sentence |
denotes |
The 47 kDa isoform was detected in silver stained gels and its identity with annexin A7 confirmed by peptide mass fingerprinting (data not shown). |
T2553 |
7720-7723 |
DT |
denotes |
The |
T2606 |
8360-8365 |
IN |
denotes |
along |
T2607 |
8366-8370 |
IN |
denotes |
with |
T2608 |
8371-8375 |
NN |
denotes |
raft |
T2609 |
8376-8383 |
NNS |
denotes |
domains |
T2610 |
8383-8385 |
, |
denotes |
, |
T2611 |
8385-8392 |
NN |
denotes |
annexin |
T2612 |
8393-8395 |
NN |
denotes |
A7 |
T2613 |
8402-8403 |
DT |
denotes |
a |
T2614 |
8404-8408 |
NN |
denotes |
role |
T2615 |
8409-8411 |
IN |
denotes |
in |
T2616 |
8412-8420 |
NN |
denotes |
membrane |
T2617 |
8421-8433 |
NN |
denotes |
organization |
T2618 |
8434-8437 |
CC |
denotes |
and |
T2619 |
8438-8441 |
DT |
denotes |
the |
T2620 |
8455-8462 |
NN |
denotes |
process |
T2621 |
8442-8454 |
NN |
denotes |
vesiculation |
T2622 |
8462-8463 |
. |
denotes |
. |
T2623 |
8463-8598 |
sentence |
denotes |
To examine this, we analysed the ability of red blood cells derived from the annexin A7 knock out mice (anxA7-/-) to form exovesicles. |
T2624 |
8464-8466 |
TO |
denotes |
To |
T2625 |
8467-8474 |
VB |
denotes |
examine |
T2626 |
8484-8492 |
VBD |
denotes |
analysed |
T2627 |
8475-8479 |
DT |
denotes |
this |
T2628 |
8479-8481 |
, |
denotes |
, |
T2629 |
8481-8483 |
PRP |
denotes |
we |
T2630 |
8493-8496 |
DT |
denotes |
the |
T2631 |
8497-8504 |
NN |
denotes |
ability |
T2632 |
8505-8507 |
IN |
denotes |
of |
T2633 |
8508-8511 |
JJ |
denotes |
red |
T2634 |
8518-8523 |
NNS |
denotes |
cells |
T2635 |
8512-8517 |
NN |
denotes |
blood |
T2636 |
8524-8531 |
VBN |
denotes |
derived |
T2637 |
8532-8536 |
IN |
denotes |
from |
T2638 |
8537-8540 |
DT |
denotes |
the |
T2639 |
8562-8566 |
NNS |
denotes |
mice |
T2640 |
8541-8548 |
NN |
denotes |
annexin |
T2641 |
8549-8551 |
NN |
denotes |
A7 |
T2642 |
8552-8557 |
NN |
denotes |
knock |
T2643 |
8558-8561 |
NN |
denotes |
out |
T2644 |
8567-8568 |
-LRB- |
denotes |
( |
T2645 |
8568-8573 |
NN |
denotes |
anxA7 |
T2646 |
8573-8574 |
SYM |
denotes |
- |
T2647 |
8574-8575 |
HYPH |
denotes |
/ |
T2648 |
8575-8576 |
SYM |
denotes |
- |
T2649 |
8576-8577 |
-RRB- |
denotes |
) |
T2650 |
8578-8580 |
TO |
denotes |
to |
T2651 |
8581-8585 |
VB |
denotes |
form |
T2652 |
8586-8597 |
NNS |
denotes |
exovesicles |
T2653 |
8597-8598 |
. |
denotes |
. |
T2654 |
8598-8734 |
sentence |
denotes |
Because of better accessibility and larger available amounts that led to clear results, we included our data obtained with human blood. |
T2655 |
8599-8606 |
IN |
denotes |
Because |
T2656 |
8690-8698 |
VBD |
denotes |
included |
T2657 |
8607-8609 |
IN |
denotes |
of |
T2658 |
8610-8616 |
JJR |
denotes |
better |
T2659 |
8617-8630 |
NN |
denotes |
accessibility |
T2660 |
8631-8634 |
CC |
denotes |
and |
T2661 |
8635-8641 |
JJR |
denotes |
larger |
T2662 |
8652-8659 |
NNS |
denotes |
amounts |
T2663 |
8642-8651 |
JJ |
denotes |
available |
T2664 |
8660-8664 |
WDT |
denotes |
that |
T2665 |
8665-8668 |
VBD |
denotes |
led |
T2666 |
8669-8671 |
IN |
denotes |
to |
T2667 |
8672-8677 |
JJ |
denotes |
clear |
T2668 |
8678-8685 |
NNS |
denotes |
results |
T2669 |
8685-8687 |
, |
denotes |
, |
T2670 |
8687-8689 |
PRP |
denotes |
we |
T2671 |
8699-8702 |
PRP$ |
denotes |
our |
T2672 |
8703-8707 |
NNS |
denotes |
data |
T2673 |
8708-8716 |
VBN |
denotes |
obtained |
T2674 |
8717-8721 |
IN |
denotes |
with |
T2675 |
8722-8727 |
JJ |
denotes |
human |
T2676 |
8728-8733 |
NN |
denotes |
blood |
T2677 |
8733-8734 |
. |
denotes |
. |
T2678 |
8734-8951 |
sentence |
denotes |
Independent of the presence of annexin A7 both types of exovesicles, micro- and nanovesicles, were released after Ca2+/ionophore treatment as determined by acetylcholine esterase activity and microscopic examination. |
T2679 |
8735-8746 |
JJ |
denotes |
Independent |
T2680 |
8834-8842 |
VBN |
denotes |
released |
T2681 |
8747-8749 |
IN |
denotes |
of |
T2682 |
8750-8753 |
DT |
denotes |
the |
T2683 |
8754-8762 |
NN |
denotes |
presence |
T2684 |
8763-8765 |
IN |
denotes |
of |
T2685 |
8766-8773 |
NN |
denotes |
annexin |
T2686 |
8774-8776 |
NN |
denotes |
A7 |
T2687 |
8777-8781 |
DT |
denotes |
both |
T2688 |
8782-8787 |
NNS |
denotes |
types |
T2689 |
8788-8790 |
IN |
denotes |
of |
T2690 |
8791-8802 |
NNS |
denotes |
exovesicles |
T2691 |
8802-8804 |
, |
denotes |
, |
T2692 |
8804-8809 |
AFX |
denotes |
micro |
T2693 |
8815-8827 |
NNS |
denotes |
nanovesicles |
T2694 |
8809-8810 |
HYPH |
denotes |
- |
T2695 |
8811-8814 |
CC |
denotes |
and |
T2696 |
8827-8829 |
, |
denotes |
, |
T2697 |
8829-8833 |
VBD |
denotes |
were |
T2698 |
8843-8848 |
IN |
denotes |
after |
T2699 |
8849-8853 |
NN |
denotes |
Ca2+ |
T2700 |
8854-8863 |
NN |
denotes |
ionophore |
T2701 |
8853-8854 |
HYPH |
denotes |
/ |
T2702 |
8864-8873 |
NN |
denotes |
treatment |
T2703 |
8874-8876 |
IN |
denotes |
as |
T2704 |
8877-8887 |
VBN |
denotes |
determined |
T2705 |
8888-8890 |
IN |
denotes |
by |
T2706 |
8891-8904 |
NN |
denotes |
acetylcholine |
T2707 |
8905-8913 |
NN |
denotes |
esterase |
T2708 |
8914-8922 |
NN |
denotes |
activity |
T2709 |
8923-8926 |
CC |
denotes |
and |
T2710 |
8927-8938 |
JJ |
denotes |
microscopic |
T2711 |
8939-8950 |
NN |
denotes |
examination |
T2712 |
8950-8951 |
. |
denotes |
. |
T2713 |
8951-9047 |
sentence |
denotes |
Annexin A7 is present in both vesicle types where it is more enriched in nanovesicles (Fig. 2). |
T2714 |
8952-8959 |
NN |
denotes |
Annexin |
T2715 |
8960-8962 |
NN |
denotes |
A7 |
T2716 |
8963-8965 |
VBZ |
denotes |
is |
T2717 |
8966-8973 |
JJ |
denotes |
present |
T2718 |
8974-8976 |
IN |
denotes |
in |
T2719 |
8977-8981 |
CC |
denotes |
both |
T2720 |
8990-8995 |
NNS |
denotes |
types |
T2721 |
8982-8989 |
NN |
denotes |
vesicle |
T2722 |
8996-9001 |
WRB |
denotes |
where |
T2723 |
9005-9007 |
VBZ |
denotes |
is |
T2724 |
9002-9004 |
PRP |
denotes |
it |
T2725 |
9008-9012 |
RBR |
denotes |
more |
T2726 |
9013-9021 |
JJ |
denotes |
enriched |
T2727 |
9022-9024 |
IN |
denotes |
in |
T2728 |
9025-9037 |
NNS |
denotes |
nanovesicles |
T2729 |
9038-9039 |
-LRB- |
denotes |
( |
T2730 |
9039-9043 |
NN |
denotes |
Fig. |
T2731 |
9044-9045 |
CD |
denotes |
2 |
T2732 |
9045-9046 |
-RRB- |
denotes |
) |
T2733 |
9046-9047 |
. |
denotes |
. |
T2734 |
9047-9102 |
sentence |
denotes |
The 51 kDa isoform is only detectable in nanovesicles. |
T2735 |
9048-9051 |
DT |
denotes |
The |
T2736 |
9059-9066 |
NN |
denotes |
isoform |
T2737 |
9052-9054 |
CD |
denotes |
51 |
T2738 |
9055-9058 |
NN |
denotes |
kDa |
T2739 |
9067-9069 |
VBZ |
denotes |
is |
T2740 |
9070-9074 |
RB |
denotes |
only |
T2741 |
9075-9085 |
JJ |
denotes |
detectable |
T2742 |
9086-9088 |
IN |
denotes |
in |
T2743 |
9089-9101 |
NNS |
denotes |
nanovesicles |
T2744 |
9101-9102 |
. |
denotes |
. |
T2745 |
9102-9316 |
sentence |
denotes |
The quantity of both vesicle types did not differ between wild type and knock out red blood cell vesicles as determined by the AChE-values (A405 nanovesicles: ~0.18; A405 microvesicles: ~0.9; both, for wt and ko). |
T2746 |
9103-9106 |
DT |
denotes |
The |
T2747 |
9107-9115 |
NN |
denotes |
quantity |
T2748 |
9146-9152 |
VB |
denotes |
differ |
T2749 |
9116-9118 |
IN |
denotes |
of |
T2750 |
9119-9123 |
DT |
denotes |
both |
T2751 |
9132-9137 |
NNS |
denotes |
types |
T2752 |
9124-9131 |
NN |
denotes |
vesicle |
T2753 |
9138-9141 |
VBD |
denotes |
did |
T2754 |
9142-9145 |
RB |
denotes |
not |
T2755 |
9153-9160 |
IN |
denotes |
between |
T2756 |
9161-9165 |
JJ |
denotes |
wild |
T2757 |
9166-9170 |
NN |
denotes |
type |
T2758 |
9200-9208 |
NNS |
denotes |
vesicles |
T2759 |
9171-9174 |
CC |
denotes |
and |
T2760 |
9175-9180 |
NN |
denotes |
knock |
T2761 |
9181-9184 |
NN |
denotes |
out |
T2762 |
9185-9188 |
JJ |
denotes |
red |
T2763 |
9195-9199 |
NN |
denotes |
cell |
T2764 |
9189-9194 |
NN |
denotes |
blood |
T2765 |
9209-9211 |
IN |
denotes |
as |
T2766 |
9212-9222 |
VBN |
denotes |
determined |
T2767 |
9223-9225 |
IN |
denotes |
by |
T2768 |
9226-9229 |
DT |
denotes |
the |
T2769 |
9235-9241 |
NNS |
denotes |
values |
T2770 |
9230-9234 |
NN |
denotes |
AChE |
T2771 |
9234-9235 |
HYPH |
denotes |
- |
T2772 |
9242-9243 |
-LRB- |
denotes |
( |
T2773 |
9295-9299 |
DT |
denotes |
both |
T2774 |
9243-9247 |
NN |
denotes |
A405 |
T2775 |
9248-9260 |
NNS |
denotes |
nanovesicles |
T2776 |
9263-9267 |
CD |
denotes |
0.18 |
T2777 |
9260-9262 |
: |
denotes |
: |
T2778 |
9262-9263 |
SYM |
denotes |
~ |
T2779 |
9267-9268 |
: |
denotes |
; |
T2780 |
9269-9273 |
NN |
denotes |
A405 |
T2781 |
9274-9287 |
NNS |
denotes |
microvesicles |
T2782 |
9290-9293 |
CD |
denotes |
0.9 |
T2783 |
9287-9289 |
: |
denotes |
: |
T2784 |
9289-9290 |
SYM |
denotes |
~ |
T2785 |
9293-9294 |
: |
denotes |
; |
T2786 |
9299-9301 |
, |
denotes |
, |
T2787 |
9301-9304 |
IN |
denotes |
for |
T2788 |
9305-9307 |
NN |
denotes |
wt |
T2789 |
9308-9311 |
CC |
denotes |
and |
T2790 |
9312-9314 |
NN |
denotes |
ko |
T2791 |
9314-9315 |
-RRB- |
denotes |
) |
T2832 |
9500-9503 |
DT |
denotes |
the |
T2833 |
9504-9512 |
NN |
denotes |
presence |
T2834 |
9513-9515 |
IN |
denotes |
of |
T2835 |
9516-9522 |
NN |
denotes |
sorcin |
T2836 |
9523-9525 |
IN |
denotes |
in |
T2837 |
9526-9530 |
JJ |
denotes |
wild |
T2838 |
9531-9535 |
NN |
denotes |
type |
T2839 |
9547-9555 |
NNS |
denotes |
vesicles |
T2840 |
9536-9539 |
CC |
denotes |
and |
T2841 |
9540-9546 |
JJ |
denotes |
mutant |
T2842 |
9556-9558 |
PRP |
denotes |
we |
T2843 |
9565-9569 |
IN |
denotes |
that |
T2844 |
9593-9600 |
VBN |
denotes |
reduced |
T2845 |
9570-9573 |
DT |
denotes |
the |
T2846 |
9581-9587 |
NNS |
denotes |
levels |
T2847 |
9574-9580 |
NN |
denotes |
sorcin |
T2848 |
9588-9592 |
VBD |
denotes |
were |
T2849 |
9601-9603 |
IN |
denotes |
in |
T2850 |
9604-9607 |
DT |
denotes |
the |
T2851 |
9615-9627 |
NNS |
denotes |
nanovesicles |
T2852 |
9608-9614 |
JJ |
denotes |
mutant |
T2853 |
9628-9629 |
-LRB- |
denotes |
( |
T2854 |
9629-9633 |
NN |
denotes |
Fig. |
T2855 |
9634-9635 |
CD |
denotes |
2 |
T2856 |
9635-9636 |
-RRB- |
denotes |
) |
T2857 |
9636-9637 |
. |
denotes |
. |
T2880 |
10388-10396 |
NNS |
denotes |
isoforms |
T2881 |
10397-10401 |
VBD |
denotes |
were |
T2882 |
10402-10409 |
JJ |
denotes |
present |
T2883 |
10410-10412 |
IN |
denotes |
in |
T2884 |
10413-10416 |
DT |
denotes |
the |
T2885 |
10425-10433 |
NN |
denotes |
fraction |
T2886 |
10417-10424 |
JJ |
denotes |
soluble |
T2887 |
10434-10436 |
RB |
denotes |
as |
T2888 |
10442-10444 |
IN |
denotes |
as |
T2889 |
10437-10441 |
RB |
denotes |
well |
T2890 |
10445-10447 |
IN |
denotes |
in |
T2891 |
10448-10451 |
DT |
denotes |
the |
T2892 |
10461-10470 |
NNS |
denotes |
fractions |
T2893 |
10452-10460 |
NN |
denotes |
gradient |
T2894 |
10471-10476 |
WRB |
denotes |
where |
T2895 |
10486-10493 |
VBN |
denotes |
assumed |
T2896 |
10477-10481 |
PRP |
denotes |
they |
T2897 |
10482-10485 |
VBP |
denotes |
are |
T2898 |
10494-10496 |
TO |
denotes |
to |
T2899 |
10500-10510 |
VBN |
denotes |
associated |
T2900 |
10497-10499 |
VB |
denotes |
be |
T2901 |
10511-10515 |
IN |
denotes |
with |
T2902 |
10516-10525 |
NNS |
denotes |
membranes |
T2903 |
10525-10526 |
. |
denotes |
. |
T2904 |
10526-10622 |
sentence |
denotes |
These membranes are exclusively plasma membranes as red blood cells are free of any organelles. |
T2905 |
10527-10532 |
DT |
denotes |
These |
T2906 |
10533-10542 |
NNS |
denotes |
membranes |
T2907 |
10543-10546 |
VBP |
denotes |
are |
T2908 |
10547-10558 |
RB |
denotes |
exclusively |
T2909 |
10559-10565 |
NN |
denotes |
plasma |
T2910 |
10566-10575 |
NNS |
denotes |
membranes |
T2911 |
10576-10578 |
IN |
denotes |
as |
T2912 |
10595-10598 |
VBP |
denotes |
are |
T2913 |
10579-10582 |
JJ |
denotes |
red |
T2914 |
10589-10594 |
NNS |
denotes |
cells |
T2915 |
10583-10588 |
NN |
denotes |
blood |
T2916 |
10599-10603 |
JJ |
denotes |
free |
T2917 |
10604-10606 |
IN |
denotes |
of |
T2918 |
10607-10610 |
DT |
denotes |
any |
T2919 |
10611-10621 |
NNS |
denotes |
organelles |
T2920 |
10621-10622 |
. |
denotes |
. |
T2921 |
10622-10692 |
sentence |
denotes |
The distribution was however not homogeneous throughout the gradient. |
T2922 |
10623-10626 |
DT |
denotes |
The |
T2923 |
10627-10639 |
NN |
denotes |
distribution |
T2924 |
10640-10643 |
VBD |
denotes |
was |
T2925 |
10644-10651 |
RB |
denotes |
however |
T2926 |
10652-10655 |
RB |
denotes |
not |
T2927 |
10656-10667 |
JJ |
denotes |
homogeneous |
T2928 |
10668-10678 |
IN |
denotes |
throughout |
T2929 |
10679-10682 |
DT |
denotes |
the |
T2930 |
10683-10691 |
NN |
denotes |
gradient |
T2931 |
10691-10692 |
. |
denotes |
. |
T2932 |
10692-10850 |
sentence |
denotes |
This could reflect the binding of annexin A7 to membrane subdomains that have different lipid or lipid/protein composition as discussed by Salzer et al. [7]. |
T2933 |
10693-10697 |
DT |
denotes |
This |
T2934 |
10704-10711 |
VB |
denotes |
reflect |
T2935 |
10698-10703 |
MD |
denotes |
could |
T2936 |
10712-10715 |
DT |
denotes |
the |
T2937 |
10716-10723 |
NN |
denotes |
binding |
T2938 |
10724-10726 |
IN |
denotes |
of |
T2939 |
10727-10734 |
NN |
denotes |
annexin |
T2940 |
10735-10737 |
NN |
denotes |
A7 |
T2941 |
10738-10740 |
IN |
denotes |
to |
T2942 |
10741-10749 |
NN |
denotes |
membrane |
T2943 |
10750-10760 |
NNS |
denotes |
subdomains |
T2944 |
10761-10765 |
WDT |
denotes |
that |
T2945 |
10766-10770 |
VBP |
denotes |
have |
T2946 |
10771-10780 |
JJ |
denotes |
different |
T2947 |
10804-10815 |
NN |
denotes |
composition |
T2948 |
10781-10786 |
NN |
denotes |
lipid |
T2949 |
10787-10789 |
CC |
denotes |
or |
T2950 |
10790-10795 |
NN |
denotes |
lipid |
T2951 |
10796-10803 |
NN |
denotes |
protein |
T2952 |
10795-10796 |
HYPH |
denotes |
/ |
T2953 |
10816-10818 |
IN |
denotes |
as |
T2954 |
10819-10828 |
VBN |
denotes |
discussed |
T2955 |
10829-10831 |
IN |
denotes |
by |
T2956 |
10832-10838 |
NNP |
denotes |
Salzer |
T2957 |
10839-10841 |
FW |
denotes |
et |
T2958 |
10842-10845 |
FW |
denotes |
al. |
T2959 |
10846-10847 |
-LRB- |
denotes |
[ |
T2960 |
10847-10848 |
CD |
denotes |
7 |
T2961 |
10848-10849 |
-RRB- |
denotes |
] |
T2962 |
10849-10850 |
. |
denotes |
. |
T2963 |
10850-10928 |
sentence |
denotes |
Likewise, sorcin does not exhibit a homogeneous distribution in the gradient. |
T2964 |
10851-10859 |
RB |
denotes |
Likewise |
T2965 |
10877-10884 |
VB |
denotes |
exhibit |
T2966 |
10859-10861 |
, |
denotes |
, |
T2967 |
10861-10867 |
NN |
denotes |
sorcin |
T2968 |
10868-10872 |
VBZ |
denotes |
does |
T2969 |
10873-10876 |
RB |
denotes |
not |
T2970 |
10885-10886 |
DT |
denotes |
a |
T2971 |
10899-10911 |
NN |
denotes |
distribution |
T2972 |
10887-10898 |
JJ |
denotes |
homogeneous |
T2973 |
10912-10914 |
IN |
denotes |
in |
T2974 |
10915-10918 |
DT |
denotes |
the |
T2975 |
10919-10927 |
NN |
denotes |
gradient |
T2976 |
10927-10928 |
. |
denotes |
. |
T2977 |
10928-11017 |
sentence |
denotes |
Moreover, it segregates into vesicles which are not associated with annexin A7 (Fig. 3). |
T2978 |
10929-10937 |
RB |
denotes |
Moreover |
T2979 |
10942-10952 |
VBZ |
denotes |
segregates |
T2980 |
10937-10939 |
, |
denotes |
, |
T2981 |
10939-10941 |
PRP |
denotes |
it |
T2982 |
10953-10957 |
IN |
denotes |
into |
T2983 |
10958-10966 |
NNS |
denotes |
vesicles |
T2984 |
10967-10972 |
WDT |
denotes |
which |
T2985 |
10981-10991 |
VBN |
denotes |
associated |
T2986 |
10973-10976 |
VBP |
denotes |
are |
T2987 |
10977-10980 |
RB |
denotes |
not |
T2988 |
10992-10996 |
IN |
denotes |
with |
T2989 |
10997-11004 |
NN |
denotes |
annexin |
T2990 |
11005-11007 |
NN |
denotes |
A7 |
T2991 |
11008-11009 |
-LRB- |
denotes |
( |
T2992 |
11009-11013 |
NN |
denotes |
Fig. |
T2993 |
11014-11015 |
CD |
denotes |
3 |
T2994 |
11015-11016 |
-RRB- |
denotes |
) |
T2995 |
11016-11017 |
. |
denotes |
. |
T2996 |
11017-11110 |
sentence |
denotes |
We also tested platelets for annexin A7 expression and detected the 47 kDa isoform (Fig. 1). |
T2997 |
11018-11020 |
PRP |
denotes |
We |
T2998 |
11026-11032 |
VBD |
denotes |
tested |
T2999 |
11021-11025 |
RB |
denotes |
also |
T3000 |
11033-11042 |
NNS |
denotes |
platelets |
T3001 |
11043-11046 |
IN |
denotes |
for |
T3002 |
11047-11054 |
NN |
denotes |
annexin |
T3003 |
11055-11057 |
NN |
denotes |
A7 |
T3004 |
11058-11068 |
NN |
denotes |
expression |
T3005 |
11069-11072 |
CC |
denotes |
and |
T3006 |
11073-11081 |
VBD |
denotes |
detected |
T3007 |
11082-11085 |
DT |
denotes |
the |
T3008 |
11093-11100 |
NN |
denotes |
isoform |
T3009 |
11086-11088 |
CD |
denotes |
47 |
T3010 |
11089-11092 |
NN |
denotes |
kDa |
T3011 |
11101-11102 |
-LRB- |
denotes |
( |
T3012 |
11102-11106 |
NN |
denotes |
Fig. |
T3013 |
11107-11108 |
CD |
denotes |
1 |
T3014 |
11108-11109 |
-RRB- |
denotes |
) |
T3015 |
11109-11110 |
. |
denotes |
. |
T3280 |
11478-11481 |
DT |
denotes |
The |
T3281 |
11482-11486 |
NN |
denotes |
lack |
T3282 |
11501-11508 |
VBZ |
denotes |
changes |
T3283 |
11487-11489 |
IN |
denotes |
of |
T3284 |
11490-11497 |
NN |
denotes |
annexin |
T3285 |
11498-11500 |
NN |
denotes |
A7 |
T3286 |
11509-11512 |
JJ |
denotes |
red |
T3287 |
11519-11523 |
NN |
denotes |
cell |
T3288 |
11513-11518 |
NN |
denotes |
blood |
T3289 |
11524-11534 |
NN |
denotes |
morphology |
T3290 |
11535-11538 |
CC |
denotes |
and |
T3291 |
11539-11546 |
JJ |
denotes |
osmotic |
T3292 |
11547-11557 |
NN |
denotes |
resistance |
T3293 |
11557-11743 |
sentence |
denotes |
As annexin A7 is a component of red blood cell vesicles we studied the consequences of the loss of the protein in the anxA7-/-mouse for red blood cell morphology and osmotic resistance. |
T3294 |
11558-11560 |
IN |
denotes |
As |
T3295 |
11572-11574 |
VBZ |
denotes |
is |
T3296 |
11561-11568 |
NN |
denotes |
annexin |
T3297 |
11569-11571 |
NN |
denotes |
A7 |
T3298 |
11617-11624 |
VBD |
denotes |
studied |
T3299 |
11575-11576 |
DT |
denotes |
a |
T3300 |
11577-11586 |
NN |
denotes |
component |
T3301 |
11587-11589 |
IN |
denotes |
of |
T3302 |
11590-11593 |
JJ |
denotes |
red |
T3303 |
11600-11604 |
NN |
denotes |
cell |
T3304 |
11594-11599 |
NN |
denotes |
blood |
T3305 |
11605-11613 |
NNS |
denotes |
vesicles |
T3306 |
11614-11616 |
PRP |
denotes |
we |
T3307 |
11625-11628 |
DT |
denotes |
the |
T3308 |
11629-11641 |
NNS |
denotes |
consequences |
T3309 |
11642-11644 |
IN |
denotes |
of |
T3310 |
11645-11648 |
DT |
denotes |
the |
T3311 |
11649-11653 |
NN |
denotes |
loss |
T3312 |
11654-11656 |
IN |
denotes |
of |
T3313 |
11657-11660 |
DT |
denotes |
the |
T3314 |
11661-11668 |
NN |
denotes |
protein |
T3315 |
11669-11671 |
IN |
denotes |
in |
T3316 |
11672-11675 |
DT |
denotes |
the |
T3317 |
11684-11689 |
NN |
denotes |
mouse |
T3318 |
11676-11681 |
NN |
denotes |
anxA7 |
T3319 |
11681-11682 |
SYM |
denotes |
- |
T3320 |
11682-11683 |
HYPH |
denotes |
/ |
T3321 |
11683-11684 |
SYM |
denotes |
- |
T3322 |
11690-11693 |
IN |
denotes |
for |
T3323 |
11694-11697 |
JJ |
denotes |
red |
T3324 |
11704-11708 |
NN |
denotes |
cell |
T3325 |
11698-11703 |
NN |
denotes |
blood |
T3326 |
11709-11719 |
NN |
denotes |
morphology |
T3327 |
11720-11723 |
CC |
denotes |
and |
T3328 |
11724-11731 |
JJ |
denotes |
osmotic |
T3329 |
11732-11742 |
NN |
denotes |
resistance |
T3330 |
11742-11743 |
. |
denotes |
. |
T3331 |
11743-11916 |
sentence |
denotes |
In a standard 'blood smear' we did not note a deformation of the cells, however dark field microscopy revealed changes in shape and diameter of the anxA7-/-red blood cells. |
T3332 |
11744-11746 |
IN |
denotes |
In |
T3333 |
11846-11854 |
VBD |
denotes |
revealed |
T3334 |
11747-11748 |
DT |
denotes |
a |
T3335 |
11765-11770 |
NN |
denotes |
smear |
T3336 |
11749-11757 |
JJ |
denotes |
standard |
T3337 |
11758-11759 |
`` |
denotes |
' |
T3338 |
11759-11764 |
NN |
denotes |
blood |
T3339 |
11770-11771 |
'' |
denotes |
' |
T3340 |
11772-11774 |
PRP |
denotes |
we |
T3341 |
11783-11787 |
VB |
denotes |
note |
T3342 |
11775-11778 |
VBD |
denotes |
did |
T3343 |
11779-11782 |
RB |
denotes |
not |
T3344 |
11788-11789 |
DT |
denotes |
a |
T3345 |
11790-11801 |
NN |
denotes |
deformation |
T3346 |
11802-11804 |
IN |
denotes |
of |
T3347 |
11805-11808 |
DT |
denotes |
the |
T3348 |
11809-11814 |
NNS |
denotes |
cells |
T3349 |
11814-11816 |
, |
denotes |
, |
T3350 |
11816-11823 |
RB |
denotes |
however |
T3351 |
11824-11828 |
JJ |
denotes |
dark |
T3352 |
11829-11834 |
NN |
denotes |
field |
T3353 |
11835-11845 |
NN |
denotes |
microscopy |
T3354 |
11855-11862 |
NNS |
denotes |
changes |
T3355 |
11863-11865 |
IN |
denotes |
in |
T3382 |
11994-11996 |
IN |
denotes |
to |
T3383 |
11997-12001 |
JJ |
denotes |
wild |
T3384 |
12002-12006 |
NN |
denotes |
type |
T3385 |
12007-12011 |
IN |
denotes |
with |
T3386 |
12012-12015 |
CD |
denotes |
5.7 |
T3387 |
12016-12018 |
NNS |
denotes |
μm |
T3388 |
12019-12020 |
-LRB- |
denotes |
( |
T3389 |
12035-12037 |
CD |
denotes |
40 |
T3390 |
12020-12021 |
NN |
denotes |
p |
T3391 |
12024-12029 |
CD |
denotes |
0.015 |
T3392 |
12022-12023 |
SYM |
denotes |
= |
T3393 |
12029-12031 |
, |
denotes |
, |
T3394 |
12031-12032 |
NN |
denotes |
n |
T3395 |
12033-12034 |
SYM |
denotes |
= |
T3396 |
12037-12038 |
-RRB- |
denotes |
) |
T3397 |
12038-12040 |
, |
denotes |
, |
T3398 |
12040-12041 |
DT |
denotes |
a |
T3399 |
12078-12088 |
NN |
denotes |
impression |
T3400 |
12042-12052 |
RB |
denotes |
remarkably |
T3401 |
12053-12058 |
JJR |
denotes |
lower |
T3402 |
12059-12069 |
VBN |
denotes |
emphasized |
T3403 |
12070-12077 |
JJ |
denotes |
central |
T3404 |
12089-12092 |
CC |
denotes |
and |
T3405 |
12093-12094 |
DT |
denotes |
a |
T3406 |
12105-12110 |
NN |
denotes |
shape |
T3407 |
12095-12099 |
RBR |
denotes |
more |
T3408 |
12100-12104 |
JJ |
denotes |
flat |
T3409 |
12111-12112 |
-LRB- |
denotes |
( |
T3410 |
12112-12116 |
NN |
denotes |
Fig. |
T3411 |
12117-12118 |
CD |
denotes |
4 |
T3412 |
12118-12119 |
-RRB- |
denotes |
) |
T3413 |
12119-12120 |
. |
denotes |
. |
T3414 |
12120-12225 |
sentence |
denotes |
No significant change in the mean corpuscular volume (MCV) was measured with the ADVIA 120 cell counter. |
T3415 |
12121-12123 |
DT |
denotes |
No |
T3416 |
12136-12142 |
NN |
denotes |
change |
T3417 |
12124-12135 |
JJ |
denotes |
significant |
T3418 |
12184-12192 |
VBN |
denotes |
measured |
T3419 |
12143-12145 |
IN |
denotes |
in |
T3420 |
12146-12149 |
DT |
denotes |
the |
T3421 |
12167-12173 |
NN |
denotes |
volume |
T3422 |
12150-12154 |
JJ |
denotes |
mean |
T3423 |
12155-12166 |
JJ |
denotes |
corpuscular |
T3424 |
12174-12175 |
-LRB- |
denotes |
( |
T3425 |
12175-12178 |
NN |
denotes |
MCV |
T3426 |
12178-12179 |
-RRB- |
denotes |
) |
T3427 |
12180-12183 |
VBD |
denotes |
was |
T3428 |
12193-12197 |
IN |
denotes |
with |
T3429 |
12198-12201 |
DT |
denotes |
the |
T3430 |
12217-12224 |
NN |
denotes |
counter |
T3431 |
12202-12207 |
NN |
denotes |
ADVIA |
T3432 |
12208-12211 |
CD |
denotes |
120 |
T3433 |
12212-12216 |
NN |
denotes |
cell |
T3434 |
12224-12225 |
. |
denotes |
. |
T3435 |
12225-12226 |
sentence |
denotes |
|
T3475 |
12727-12731 |
VB |
denotes |
play |
T3476 |
12710-12717 |
NN |
denotes |
annexin |
T3477 |
12718-12720 |
NN |
denotes |
A7 |
T3478 |
12721-12726 |
MD |
denotes |
might |
T3479 |
12732-12733 |
DT |
denotes |
a |
T3480 |
12734-12738 |
NN |
denotes |
role |
T3481 |
12738-12739 |
. |
denotes |
. |
T3482 |
12739-12908 |
sentence |
denotes |
The osmotic resistance, which is the resistance towards changes in the extracellular ionic strength, is a convenient assay for analysis of the red blood cell integrity. |
T3483 |
12740-12743 |
DT |
denotes |
The |
T3484 |
12752-12762 |
NN |
denotes |
resistance |
T3485 |
12744-12751 |
JJ |
denotes |
osmotic |
T3486 |
12841-12843 |
VBZ |
denotes |
is |
T3487 |
12762-12764 |
, |
denotes |
, |
T3488 |
12764-12769 |
WDT |
denotes |
which |
T3489 |
12770-12772 |
VBZ |
denotes |
is |
T3490 |
12773-12776 |
DT |
denotes |
the |
T3491 |
12777-12787 |
NN |
denotes |
resistance |
T3492 |
12788-12795 |
IN |
denotes |
towards |
T3493 |
12796-12803 |
NNS |
denotes |
changes |
T3494 |
12804-12806 |
IN |
denotes |
in |
T3495 |
12807-12810 |
DT |
denotes |
the |
T3496 |
12831-12839 |
NN |
denotes |
strength |
T3497 |
12811-12824 |
JJ |
denotes |
extracellular |
T3498 |
12825-12830 |
JJ |
denotes |
ionic |
T3499 |
12839-12841 |
, |
denotes |
, |
T3500 |
12844-12845 |
DT |
denotes |
a |
T3501 |
12857-12862 |
NN |
denotes |
assay |
T3502 |
12846-12856 |
JJ |
denotes |
convenient |
T3503 |
12863-12866 |
IN |
denotes |
for |
T3504 |
12867-12875 |
NN |
denotes |
analysis |
T3505 |
12876-12878 |
IN |
denotes |
of |
T3506 |
12879-12882 |
DT |
denotes |
the |
T3507 |
12898-12907 |
NN |
denotes |
integrity |
T3508 |
12883-12886 |
JJ |
denotes |
red |
T3509 |
12893-12897 |
NN |
denotes |
cell |
T3510 |
12887-12892 |
NN |
denotes |
blood |
T3511 |
12907-12908 |
. |
denotes |
. |
T3512 |
12908-13075 |
sentence |
denotes |
It is measured as the sodium chloride concentration at which cellular lysis starts (minimal resistance) up to a complete lysis (maximal resistance) of the cells [33]. |
T3513 |
12909-12911 |
PRP |
denotes |
It |
T3514 |
12915-12923 |
VBN |
denotes |
measured |
T3515 |
12912-12914 |
VBZ |
denotes |
is |
T3516 |
12924-12926 |
IN |
denotes |
as |
T3517 |
12927-12930 |
DT |
denotes |
the |
T3518 |
12947-12960 |
NN |
denotes |
concentration |
T3519 |
12931-12937 |
NN |
denotes |
sodium |
T3520 |
12938-12946 |
NN |
denotes |
chloride |
T3521 |
12961-12963 |
IN |
denotes |
at |
T3522 |
12985-12991 |
VBZ |
denotes |
starts |
T3523 |
12964-12969 |
WDT |
denotes |
which |
T3524 |
12970-12978 |
JJ |
denotes |
cellular |
T3525 |
12979-12984 |
NN |
denotes |
lysis |
T3526 |
12992-12993 |
-LRB- |
denotes |
( |
T3527 |
12993-13000 |
JJ |
denotes |
minimal |
T3528 |
13001-13011 |
NN |
denotes |
resistance |
T3529 |
13011-13012 |
-RRB- |
denotes |
) |
T3530 |
13013-13015 |
IN |
denotes |
up |
T3531 |
13016-13018 |
IN |
denotes |
to |
T3532 |
13019-13020 |
DT |
denotes |
a |
T3533 |
13030-13035 |
NN |
denotes |
lysis |
T3534 |
13021-13029 |
JJ |
denotes |
complete |
T3535 |
13036-13037 |
-LRB- |
denotes |
( |
T3536 |
13037-13044 |
JJ |
denotes |
maximal |
T3537 |
13045-13055 |
NN |
denotes |
resistance |
T3538 |
13055-13056 |
-RRB- |
denotes |
) |
T3539 |
13057-13059 |
IN |
denotes |
of |
T3540 |
13060-13063 |
DT |
denotes |
the |
T3541 |
13064-13069 |
NNS |
denotes |
cells |
T3542 |
13070-13071 |
-LRB- |
denotes |
[ |
T3543 |
13071-13073 |
CD |
denotes |
33 |
T3544 |
13073-13074 |
-RRB- |
denotes |
] |
T3545 |
13074-13075 |
. |
denotes |
. |
T3546 |
13075-13226 |
sentence |
denotes |
Osmotic resistance increases with higher MCV, larger surface area and a higher degree of cellular metabolism stabilizing the intracellular ion levels. |
T3547 |
13076-13083 |
JJ |
denotes |
Osmotic |
T3548 |
13084-13094 |
NN |
denotes |
resistance |
T3549 |
13095-13104 |
VBZ |
denotes |
increases |
T3550 |
13105-13109 |
IN |
denotes |
with |
T3551 |
13110-13116 |
JJR |
denotes |
higher |
T3552 |
13117-13120 |
NN |
denotes |
MCV |
T3553 |
13120-13122 |
, |
denotes |
, |
T3554 |
13122-13128 |
JJR |
denotes |
larger |
T3555 |
13137-13141 |
NN |
denotes |
area |
T3556 |
13129-13136 |
NN |
denotes |
surface |
T3557 |
13142-13145 |
CC |
denotes |
and |
T3558 |
13146-13147 |
DT |
denotes |
a |
T3559 |
13155-13161 |
NN |
denotes |
degree |
T3560 |
13148-13154 |
JJR |
denotes |
higher |
T3561 |
13162-13164 |
IN |
denotes |
of |
T3562 |
13165-13173 |
JJ |
denotes |
cellular |
T3563 |
13174-13184 |
NN |
denotes |
metabolism |
T3564 |
13185-13196 |
VBG |
denotes |
stabilizing |
T3565 |
13197-13200 |
DT |
denotes |
the |
T3566 |
13219-13225 |
NNS |
denotes |
levels |
T3567 |
13201-13214 |
JJ |
denotes |
intracellular |
T3568 |
13215-13218 |
NN |
denotes |
ion |
T3569 |
13225-13226 |
. |
denotes |
. |
T3570 |
13226-13301 |
sentence |
denotes |
Aged red blood cells show a lowered MCV and lower osmotic resistance [33]. |
T3571 |
13227-13231 |
VBN |
denotes |
Aged |
T3572 |
13242-13247 |
NNS |
denotes |
cells |
T3573 |
13232-13235 |
JJ |
denotes |
red |
T3574 |
13236-13241 |
NN |
denotes |
blood |
T3575 |
13248-13252 |
VBP |
denotes |
show |
T3576 |
13253-13254 |
DT |
denotes |
a |
T3577 |
13263-13266 |
NN |
denotes |
MCV |
T3578 |
13255-13262 |
JJ |
denotes |
lowered |
T3579 |
13267-13270 |
CC |
denotes |
and |
T3580 |
13271-13276 |
JJR |
denotes |
lower |
T3581 |
13285-13295 |
NN |
denotes |
resistance |
T3582 |
13277-13284 |
JJ |
denotes |
osmotic |
T3583 |
13296-13297 |
-LRB- |
denotes |
[ |
T3584 |
13297-13299 |
CD |
denotes |
33 |
T3585 |
13299-13300 |
-RRB- |
denotes |
] |
T3586 |
13300-13301 |
. |
denotes |
. |
T3587 |
13301-13391 |
sentence |
denotes |
Similar properties can be observed in red blood cells with lowered membrane permeability. |
T3588 |
13302-13309 |
JJ |
denotes |
Similar |
T3589 |
13310-13320 |
NNS |
denotes |
properties |
T3590 |
13328-13336 |
VBN |
denotes |
observed |
T3591 |
13321-13324 |
MD |
denotes |
can |
T3592 |
13325-13327 |
VB |
denotes |
be |
T3593 |
13337-13339 |
IN |
denotes |
in |
T3594 |
13340-13343 |
JJ |
denotes |
red |
T3595 |
13350-13355 |
NNS |
denotes |
cells |
T3596 |
13344-13349 |
NN |
denotes |
blood |
T3597 |
13356-13360 |
IN |
denotes |
with |
T3598 |
13361-13368 |
VBN |
denotes |
lowered |
T3599 |
13378-13390 |
NN |
denotes |
permeability |
T3600 |
13369-13377 |
NN |
denotes |
membrane |
T3601 |
13390-13391 |
. |
denotes |
. |
T3602 |
13391-13510 |
sentence |
denotes |
The osmotic resistance of annexin A7 deficient red blood cells is significantly increased compared to wild type cells. |
T3603 |
13392-13395 |
DT |
denotes |
The |
T3604 |
13404-13414 |
NN |
denotes |
resistance |
T3605 |
13396-13403 |
JJ |
denotes |
osmotic |
T3606 |
13455-13457 |
VBZ |
denotes |
is |
T3607 |
13415-13417 |
IN |
denotes |
of |
T3608 |
13418-13425 |
NN |
denotes |
annexin |
T3609 |
13426-13428 |
NN |
denotes |
A7 |
T3610 |
13429-13438 |
JJ |
denotes |
deficient |
T3611 |
13449-13454 |
NNS |
denotes |
cells |
T3612 |
13439-13442 |
JJ |
denotes |
red |
T3613 |
13443-13448 |
NN |
denotes |
blood |
T3614 |
13458-13471 |
RB |
denotes |
significantly |
T3615 |
13472-13481 |
VBN |
denotes |
increased |
T3616 |
13482-13490 |
VBN |
denotes |
compared |
T3617 |
13491-13493 |
IN |
denotes |
to |
T3618 |
13494-13498 |
JJ |
denotes |
wild |
T3619 |
13499-13503 |
NN |
denotes |
type |
T3620 |
13504-13509 |
NNS |
denotes |
cells |
T3621 |
13509-13510 |
. |
denotes |
. |
T3622 |
13510-13631 |
sentence |
denotes |
The minimal resistance value of knock out cells is observed in a 0.60 % NaCl solution versus 0.65 % for wild type cells. |
T3623 |
13511-13514 |
DT |
denotes |
The |
T3624 |
13534-13539 |
NN |
denotes |
value |
T3625 |
13515-13522 |
JJ |
denotes |
minimal |
T3626 |
13523-13533 |
NN |
denotes |
resistance |
T3627 |
13562-13570 |
VBN |
denotes |
observed |
T3628 |
13540-13542 |
IN |
denotes |
of |
T3629 |
13543-13548 |
NN |
denotes |
knock |
T3630 |
13549-13552 |
NN |
denotes |
out |
T3631 |
13553-13558 |
NNS |
denotes |
cells |
T3632 |
13559-13561 |
VBZ |
denotes |
is |
T3633 |
13571-13573 |
IN |
denotes |
in |
T3634 |
13574-13575 |
DT |
denotes |
a |
T3635 |
13588-13596 |
NN |
denotes |
solution |
T3636 |
13576-13580 |
CD |
denotes |
0.60 |
T3637 |
13581-13582 |
NN |
denotes |
% |
T3638 |
13583-13587 |
NN |
denotes |
NaCl |
T3639 |
13597-13603 |
CC |
denotes |
versus |
T3640 |
13604-13608 |
CD |
denotes |
0.65 |
T3641 |
13609-13610 |
NN |
denotes |
% |
T3642 |
13611-13614 |
IN |
denotes |
for |
T3643 |
13615-13619 |
JJ |
denotes |
wild |
T3644 |
13620-13624 |
NN |
denotes |
type |
T3645 |
13625-13630 |
NNS |
denotes |
cells |
T3646 |
13630-13631 |
. |
denotes |
. |
T3647 |
13631-13781 |
sentence |
denotes |
50 % haemolysis is achieved at 0.516 % NaCl solution for knock out versus 0.564 % NaCl for wild type cells (mean values, p = 0.00066, n = 8; Fig. 5). |
T3648 |
13632-13634 |
CD |
denotes |
50 |
T3649 |
13635-13636 |
NN |
denotes |
% |
T3650 |
13637-13647 |
NN |
denotes |
haemolysis |
T3651 |
13651-13659 |
VBN |
denotes |
achieved |
T3652 |
13648-13650 |
VBZ |
denotes |
is |
T3653 |
13660-13662 |
IN |
denotes |
at |
T3654 |
13663-13668 |
CD |
denotes |
0.516 |
T3655 |
13669-13670 |
NN |
denotes |
% |
T3656 |
13676-13684 |
NN |
denotes |
solution |
T3657 |
13671-13675 |
NN |
denotes |
NaCl |
T3658 |
13685-13688 |
IN |
denotes |
for |
T3659 |
13689-13694 |
NN |
denotes |
knock |
T3660 |
13695-13698 |
NN |
denotes |
out |
T3661 |
13699-13705 |
CC |
denotes |
versus |
T3662 |
13706-13711 |
CD |
denotes |
0.564 |
T3663 |
13712-13713 |
NN |
denotes |
% |
T3664 |
13714-13718 |
NN |
denotes |
NaCl |
T3665 |
13719-13722 |
IN |
denotes |
for |
T3666 |
13723-13727 |
JJ |
denotes |
wild |
T3667 |
13728-13732 |
NN |
denotes |
type |
T3668 |
13733-13738 |
NNS |
denotes |
cells |
T3669 |
13739-13740 |
-LRB- |
denotes |
( |
T3670 |
13773-13777 |
NN |
denotes |
Fig. |
T3671 |
13740-13744 |
NN |
denotes |
mean |
T3672 |
13745-13751 |
NNS |
denotes |
values |
T3673 |
13751-13753 |
, |
denotes |
, |
T3674 |
13753-13754 |
NN |
denotes |
p |
T3675 |
13757-13764 |
CD |
denotes |
0.00066 |
T3676 |
13755-13756 |
SYM |
denotes |
= |
T3677 |
13764-13766 |
, |
denotes |
, |
T3678 |
13766-13767 |
NN |
denotes |
n |
T3679 |
13770-13771 |
CD |
denotes |
8 |
T3680 |
13768-13769 |
SYM |
denotes |
= |
T3681 |
13771-13772 |
: |
denotes |
; |
T3682 |
13778-13779 |
CD |
denotes |
5 |
T3683 |
13779-13780 |
-RRB- |
denotes |
) |
T3684 |
13780-13781 |
. |
denotes |
. |
T3685 |
13781-13885 |
sentence |
denotes |
The resistance width of the knock out cells is slightly lower (0.20 % instead of 0.25 % NaCl solution). |
T3686 |
13782-13785 |
DT |
denotes |
The |
T3687 |
13797-13802 |
NN |
denotes |
width |
T3688 |
13786-13796 |
NN |
denotes |
resistance |
T3689 |
13826-13828 |
VBZ |
denotes |
is |
T3690 |
13803-13805 |
IN |
denotes |
of |
T3691 |
13806-13809 |
DT |
denotes |
the |
T3692 |
13820-13825 |
NNS |
denotes |
cells |
T3693 |
13810-13815 |
NN |
denotes |
knock |
T3694 |
13816-13819 |
NN |
denotes |
out |
T3695 |
13829-13837 |
RB |
denotes |
slightly |
T3696 |
13838-13843 |
JJR |
denotes |
lower |
T3697 |
13844-13845 |
-LRB- |
denotes |
( |
T3698 |
13875-13883 |
NN |
denotes |
solution |
T3699 |
13845-13849 |
CD |
denotes |
0.20 |
T3700 |
13850-13851 |
NN |
denotes |
% |
T3701 |
13870-13874 |
NN |
denotes |
NaCl |
T3702 |
13852-13859 |
RB |
denotes |
instead |
T3703 |
13860-13862 |
IN |
denotes |
of |
T3704 |
13863-13867 |
CD |
denotes |
0.25 |
T3705 |
13868-13869 |
NN |
denotes |
% |
T3706 |
13883-13884 |
-RRB- |
denotes |
) |
T3707 |
13884-13885 |
. |
denotes |
. |
T3708 |
13885-14050 |
sentence |
denotes |
We also performed direct measurements of the red blood cell membrane deformability, to further characterize a possible role of annexin A7 on the membrane stability. |
T3709 |
13886-13888 |
PRP |
denotes |
We |
T3710 |
13894-13903 |
VBD |
denotes |
performed |
T3711 |
13889-13893 |
RB |
denotes |
also |
T3712 |
13904-13910 |
JJ |
denotes |
direct |
T3713 |
13911-13923 |
NNS |
denotes |
measurements |
T3714 |
13924-13926 |
IN |
denotes |
of |
T3718 |
13941-13945 |
NN |
denotes |
cell |
T3719 |
13935-13940 |
NN |
denotes |
blood |
T3720 |
13946-13954 |
NN |
denotes |
membrane |
T3721 |
13968-13970 |
, |
denotes |
, |
T3722 |
13970-13972 |
TO |
denotes |
to |
T3723 |
13981-13993 |
VB |
denotes |
characterize |
T3724 |
13973-13980 |
RBR |
denotes |
further |
T3725 |
13994-13995 |
DT |
denotes |
a |
T3726 |
14005-14009 |
NN |
denotes |
role |
T3727 |
13996-14004 |
JJ |
denotes |
possible |
T3728 |
14010-14012 |
IN |
denotes |
of |
T3729 |
14013-14020 |
NN |
denotes |
annexin |
T3730 |
14021-14023 |
NN |
denotes |
A7 |
T3731 |
14024-14026 |
IN |
denotes |
on |
T3732 |
14027-14030 |
DT |
denotes |
the |
T3733 |
14040-14049 |
NN |
denotes |
stability |
T3734 |
14031-14039 |
NN |
denotes |
membrane |
T3735 |
14049-14050 |
. |
denotes |
. |
T3736 |
14050-14175 |
sentence |
denotes |
With micropipette experiments we tried to correlate mechanical characteristics of the red blood cells with their morphology. |
T3737 |
14051-14055 |
IN |
denotes |
With |
T3738 |
14084-14089 |
VBD |
denotes |
tried |
T3739 |
14056-14068 |
NN |
denotes |
micropipette |
T3740 |
14069-14080 |
NNS |
denotes |
experiments |
T3741 |
14081-14083 |
PRP |
denotes |
we |
T3742 |
14090-14092 |
TO |
denotes |
to |
T3743 |
14093-14102 |
VB |
denotes |
correlate |
T3744 |
14103-14113 |
JJ |
denotes |
mechanical |
T3745 |
14114-14129 |
NNS |
denotes |
characteristics |
T3746 |
14130-14132 |
IN |
denotes |
of |
T3747 |
14133-14136 |
DT |
denotes |
the |
T3748 |
14147-14152 |
NNS |
denotes |
cells |
T3749 |
14137-14140 |
JJ |
denotes |
red |
T3750 |
14141-14146 |
NN |
denotes |
blood |
T3751 |
14153-14157 |
IN |
denotes |
with |
T3752 |
14158-14163 |
PRP$ |
denotes |
their |
T3753 |
14164-14174 |
NN |
denotes |
morphology |
T3754 |
14174-14175 |
. |
denotes |
. |
T3755 |
14175-14372 |
sentence |
denotes |
We could not observe any statistically significant difference of values describing membrane rigidity and lysis force in these experiments (M. Heil, B. Hoffmann and R. Merkel, unpublished results). |
T3756 |
14176-14178 |
PRP |
denotes |
We |
T3757 |
14189-14196 |
VB |
denotes |
observe |
T3758 |
14179-14184 |
MD |
denotes |
could |
T3759 |
14185-14188 |
RB |
denotes |
not |
T3760 |
14197-14200 |
DT |
denotes |
any |
T3761 |
14227-14237 |
NN |
denotes |
difference |
T3762 |
14201-14214 |
RB |
denotes |
statistically |
T3763 |
14215-14226 |
JJ |
denotes |
significant |
T3764 |
14238-14240 |
IN |
denotes |
of |
T3765 |
14241-14247 |
NNS |
denotes |
values |
T3766 |
14248-14258 |
VBG |
denotes |
describing |
T3767 |
14259-14267 |
NN |
denotes |
membrane |
T3768 |
14268-14276 |
NN |
denotes |
rigidity |
T3769 |
14277-14280 |
CC |
denotes |
and |
T3770 |
14281-14286 |
NN |
denotes |
lysis |
T3771 |
14287-14292 |
NN |
denotes |
force |
T3772 |
14293-14295 |
IN |
denotes |
in |
T3773 |
14296-14301 |
DT |
denotes |
these |
T3774 |
14302-14313 |
NNS |
denotes |
experiments |
T3775 |
14314-14315 |
-LRB- |
denotes |
( |
T3776 |
14315-14317 |
NNP |
denotes |
M. |
T3777 |
14318-14322 |
NNP |
denotes |
Heil |
T3778 |
14322-14324 |
, |
denotes |
, |
T3779 |
14324-14326 |
NNP |
denotes |
B. |
T3780 |
14327-14335 |
NNP |
denotes |
Hoffmann |
T3781 |
14336-14339 |
CC |
denotes |
and |
T3782 |
14340-14342 |
NNP |
denotes |
R. |
T3783 |
14343-14349 |
NNP |
denotes |
Merkel |
T3784 |
14349-14351 |
, |
denotes |
, |
T3785 |
14351-14362 |
JJ |
denotes |
unpublished |
T3786 |
14363-14370 |
NNS |
denotes |
results |
T3787 |
14370-14371 |
-RRB- |
denotes |
) |
T3788 |
14371-14372 |
. |
denotes |
. |
T3789 |
14372-14542 |
sentence |
denotes |
However, the distinction observed in the osmotic resistance experiments was highly significant and reflects a mean value over a high number of different red blood cells. |
T3790 |
14373-14380 |
RB |
denotes |
However |
T3791 |
14445-14448 |
VBD |
denotes |
was |
T3792 |
14380-14382 |
, |
denotes |
, |
T3793 |
14382-14385 |
DT |
denotes |
the |
T3794 |
14386-14397 |
NN |
denotes |
distinction |
T3795 |
14398-14406 |
VBN |
denotes |
observed |
T3796 |
14407-14409 |
IN |
denotes |
in |
T3797 |
14410-14413 |
DT |
denotes |
the |
T3798 |
14433-14444 |
NNS |
denotes |
experiments |
T3799 |
14414-14421 |
JJ |
denotes |
osmotic |
T3800 |
14422-14432 |
NN |
denotes |
resistance |
T3801 |
14449-14455 |
RB |
denotes |
highly |
T3802 |
14456-14467 |
JJ |
denotes |
significant |
T3803 |
14468-14471 |
CC |
denotes |
and |
T3804 |
14472-14480 |
VBZ |
denotes |
reflects |
T3805 |
14481-14482 |
DT |
denotes |
a |
T3806 |
14488-14493 |
NN |
denotes |
value |
T3807 |
14483-14487 |
JJ |
denotes |
mean |
T3808 |
14494-14498 |
IN |
denotes |
over |
T3809 |
14499-14500 |
DT |
denotes |
a |
T3810 |
14506-14512 |
NN |
denotes |
number |
T3811 |
14501-14505 |
JJ |
denotes |
high |
T3812 |
14513-14515 |
IN |
denotes |
of |
T3813 |
14516-14525 |
JJ |
denotes |
different |
T3814 |
14536-14541 |
NNS |
denotes |
cells |
T3815 |
14526-14529 |
JJ |
denotes |
red |
T3816 |
14530-14535 |
NN |
denotes |
blood |
T3817 |
14541-14542 |
. |
denotes |
. |
T3818 |
14542-15200 |
sentence |
denotes |
Figure 5 Osmotic resistance curves of red blood cells from wild type and anxA7-/- mutant mice. The osmotic resistance towards changes in the extracellular ionic strength is a convenient assay for analysis of the red blood cell integrity. It is measured as a sodium chloride concentration in which cellular lysis starts (minimal resistance) up to a complete lysis (maximal resistance) of a red blood cell. The osmotic resistance of annexin A7 deficient red blood cells is significantly increased compared to the one of wild type (p = 0.00066; n = 8). These data suggest that annexin A7 contributes to the shape of red blood cells and the osmotic resistance. |
T3819 |
15094-15099 |
DT |
denotes |
These |
T3820 |
15100-15104 |
NNS |
denotes |
data |
T3821 |
15105-15112 |
VBP |
denotes |
suggest |
T3822 |
15113-15117 |
IN |
denotes |
that |
T3823 |
15129-15140 |
VBZ |
denotes |
contributes |
T3824 |
15118-15125 |
NN |
denotes |
annexin |
T3825 |
15126-15128 |
NN |
denotes |
A7 |
T3826 |
15141-15143 |
IN |
denotes |
to |
T3827 |
15144-15147 |
DT |
denotes |
the |
T3828 |
15148-15153 |
NN |
denotes |
shape |
T3829 |
15154-15156 |
IN |
denotes |
of |
T3830 |
15157-15160 |
JJ |
denotes |
red |
T3831 |
15167-15172 |
NNS |
denotes |
cells |
T3832 |
15161-15166 |
NN |
denotes |
blood |
T3833 |
15173-15176 |
CC |
denotes |
and |
T3834 |
15177-15180 |
DT |
denotes |
the |
T3835 |
15189-15199 |
NN |
denotes |
resistance |
T3836 |
15181-15188 |
JJ |
denotes |
osmotic |
T3837 |
15199-15200 |
. |
denotes |
. |
T3838 |
15200-15378 |
sentence |
denotes |
As we have not observed a binding of annexin A7 to F-actin (data not shown) it could do so either by alteration of the membrane rigidity and/or by affecting the ion homeostasis. |
T3839 |
15201-15203 |
IN |
denotes |
As |
T3840 |
15216-15224 |
VBN |
denotes |
observed |
T3841 |
15204-15206 |
PRP |
denotes |
we |
T3842 |
15207-15211 |
VBP |
denotes |
have |
T3843 |
15212-15215 |
RB |
denotes |
not |
T3844 |
15286-15288 |
VB |
denotes |
do |
T3845 |
15225-15226 |
DT |
denotes |
a |
T3846 |
15227-15234 |
NN |
denotes |
binding |
T3847 |
15235-15237 |
IN |
denotes |
of |
T3848 |
15238-15245 |
NN |
denotes |
annexin |
T3849 |
15246-15248 |
NN |
denotes |
A7 |
T3850 |
15249-15251 |
IN |
denotes |
to |
T3851 |
15252-15253 |
NN |
denotes |
F |
T3852 |
15254-15259 |
NN |
denotes |
actin |
T3853 |
15253-15254 |
HYPH |
denotes |
- |
T3854 |
15260-15261 |
-LRB- |
denotes |
( |
T3855 |
15270-15275 |
VBN |
denotes |
shown |
T3856 |
15261-15265 |
NNS |
denotes |
data |
T3857 |
15266-15269 |
RB |
denotes |
not |
T3858 |
15275-15276 |
-RRB- |
denotes |
) |
T3859 |
15277-15279 |
PRP |
denotes |
it |
T3860 |
15280-15285 |
MD |
denotes |
could |
T3861 |
15289-15291 |
RB |
denotes |
so |
T3862 |
15292-15298 |
CC |
denotes |
either |
T3863 |
15299-15301 |
IN |
denotes |
by |
T3864 |
15302-15312 |
NN |
denotes |
alteration |
T3865 |
15313-15315 |
IN |
denotes |
of |
T3866 |
15316-15319 |
DT |
denotes |
the |
T3867 |
15329-15337 |
NN |
denotes |
rigidity |
T3868 |
15320-15328 |
NN |
denotes |
membrane |
T3869 |
15338-15341 |
CC |
denotes |
and |
T3870 |
15341-15342 |
HYPH |
denotes |
/ |
T3871 |
15342-15344 |
CC |
denotes |
or |
T3872 |
15345-15347 |
IN |
denotes |
by |
T3873 |
15348-15357 |
VBG |
denotes |
affecting |
T3874 |
15358-15361 |
DT |
denotes |
the |
T3875 |
15366-15377 |
NN |
denotes |
homeostasis |
T3876 |
15362-15365 |
NN |
denotes |
ion |
T3877 |
15377-15378 |
. |
denotes |
. |
T4078 |
15380-15383 |
DT |
denotes |
The |
T4079 |
15384-15388 |
NN |
denotes |
lack |
T4080 |
15403-15410 |
VBZ |
denotes |
affects |
T4081 |
15389-15391 |
IN |
denotes |
of |
T4082 |
15392-15399 |
NN |
denotes |
annexin |
T4083 |
15400-15402 |
NN |
denotes |
A7 |
T4084 |
15411-15418 |
JJ |
denotes |
primary |
T4085 |
15419-15430 |
NN |
denotes |
haemostasis |
T4086 |
15431-15433 |
FW |
denotes |
ex |
T4087 |
15434-15438 |
FW |
denotes |
vivo |
T4088 |
15438-15602 |
sentence |
denotes |
The use of an advanced electronic cell counter/flow cytometer (ADVIA 120) allowed us to screen other parameters as well despite of the limited murine blood volume. |
T4089 |
15439-15442 |
DT |
denotes |
The |
T4090 |
15443-15446 |
NN |
denotes |
use |
T4091 |
15513-15520 |
VBD |
denotes |
allowed |
T4092 |
15447-15449 |
IN |
denotes |
of |
T4093 |
15450-15452 |
DT |
denotes |
an |
T4094 |
15478-15485 |
NN |
denotes |
counter |
T4095 |
15453-15461 |
JJ |
denotes |
advanced |
T4096 |
15462-15472 |
JJ |
denotes |
electronic |
T4097 |
15473-15477 |
NN |
denotes |
cell |
T4098 |
15485-15486 |
HYPH |
denotes |
/ |
T4099 |
15486-15490 |
NN |
denotes |
flow |
T4100 |
15491-15500 |
NN |
denotes |
cytometer |
T4101 |
15501-15502 |
-LRB- |
denotes |
( |
T4102 |
15502-15507 |
NN |
denotes |
ADVIA |
T4103 |
15508-15511 |
CD |
denotes |
120 |
T4104 |
15511-15512 |
-RRB- |
denotes |
) |
T4105 |
15521-15523 |
PRP |
denotes |
us |
T4106 |
15527-15533 |
VB |
denotes |
screen |
T4107 |
15524-15526 |
TO |
denotes |
to |
T4108 |
15534-15539 |
JJ |
denotes |
other |
T4109 |
15540-15550 |
NNS |
denotes |
parameters |
T4110 |
15551-15553 |
RB |
denotes |
as |
T4111 |
15554-15558 |
RB |
denotes |
well |
T4112 |
15559-15566 |
IN |
denotes |
despite |
T4113 |
15567-15569 |
IN |
denotes |
of |
T4114 |
15570-15573 |
DT |
denotes |
the |
T4115 |
15595-15601 |
NN |
denotes |
volume |
T4116 |
15574-15581 |
JJ |
denotes |
limited |
T4117 |
15582-15588 |
JJ |
denotes |
murine |
T4118 |
15589-15594 |
NN |
denotes |
blood |
T4119 |
15601-15602 |
. |
denotes |
. |
T4120 |
15602-15845 |
sentence |
denotes |
We analysed leukocytes, red blood cells, haemoglobin content, haematocrit, mean cellular volume, mean cellular haemoglobin, mean cellular haemoglobin concentration, platelets, neutrophiles, lymphocytes, monocytes, eosinophiles and basophiles. |
T4121 |
15603-15605 |
PRP |
denotes |
We |
T4122 |
15606-15614 |
VBD |
denotes |
analysed |
T4123 |
15615-15625 |
NNS |
denotes |
leukocytes |
T4124 |
15625-15627 |
, |
denotes |
, |
T4125 |
15627-15630 |
JJ |
denotes |
red |
T4126 |
15637-15642 |
NNS |
denotes |
cells |
T4127 |
15631-15636 |
NN |
denotes |
blood |
T4128 |
15642-15644 |
, |
denotes |
, |
T4129 |
15644-15655 |
NN |
denotes |
haemoglobin |
T4130 |
15656-15663 |
NN |
denotes |
content |
T4131 |
15663-15665 |
, |
denotes |
, |
T4132 |
15665-15676 |
NN |
denotes |
haematocrit |
T4133 |
15676-15678 |
, |
denotes |
, |
T4134 |
15678-15682 |
JJ |
denotes |
mean |
T4135 |
15692-15698 |
NN |
denotes |
volume |
T4136 |
15683-15691 |
JJ |
denotes |
cellular |
T4137 |
15698-15700 |
, |
denotes |
, |
T4138 |
15700-15704 |
JJ |
denotes |
mean |
T4139 |
15714-15725 |
NN |
denotes |
haemoglobin |
T4140 |
15705-15713 |
JJ |
denotes |
cellular |
T4141 |
15725-15727 |
, |
denotes |
, |
T4142 |
15727-15731 |
JJ |
denotes |
mean |
T4143 |
15741-15752 |
NN |
denotes |
haemoglobin |
T4144 |
15732-15740 |
JJ |
denotes |
cellular |
T4145 |
15753-15766 |
NN |
denotes |
concentration |
T4146 |
15766-15768 |
, |
denotes |
, |
T4147 |
15768-15777 |
NNS |
denotes |
platelets |
T4148 |
15777-15779 |
, |
denotes |
, |
T4149 |
15779-15791 |
NNS |
denotes |
neutrophiles |
T4150 |
15791-15793 |
, |
denotes |
, |
T4151 |
15793-15804 |
NNS |
denotes |
lymphocytes |
T4152 |
15804-15806 |
, |
denotes |
, |
T4153 |
15806-15815 |
NNS |
denotes |
monocytes |
T4154 |
15815-15817 |
, |
denotes |
, |
T4155 |
15817-15829 |
NNS |
denotes |
eosinophiles |
T4156 |
15830-15833 |
CC |
denotes |
and |
T4157 |
15834-15844 |
NNS |
denotes |
basophiles |
T4158 |
15844-15845 |
. |
denotes |
. |
T4159 |
15845-15986 |
sentence |
denotes |
In these tests of whole blood we found only a change in the platelet numbers, whereas all other haematological parameters were not affected. |
T4160 |
15846-15848 |
IN |
denotes |
In |
T4161 |
15879-15884 |
VBD |
denotes |
found |
T4162 |
15849-15854 |
DT |
denotes |
these |
T4163 |
15855-15860 |
NNS |
denotes |
tests |
T4164 |
15861-15863 |
IN |
denotes |
of |
T4165 |
15864-15869 |
JJ |
denotes |
whole |
T4166 |
15870-15875 |
NN |
denotes |
blood |
T4167 |
15876-15878 |
PRP |
denotes |
we |
T4168 |
15885-15889 |
RB |
denotes |
only |
T4169 |
15890-15891 |
DT |
denotes |
a |
T4170 |
15892-15898 |
NN |
denotes |
change |
T4171 |
15899-15901 |
IN |
denotes |
in |
T4172 |
15902-15905 |
DT |
denotes |
the |
T4173 |
15915-15922 |
NNS |
denotes |
numbers |
T4174 |
15906-15914 |
NN |
denotes |
platelet |
T4175 |
15922-15924 |
, |
denotes |
, |
T4176 |
15924-15931 |
IN |
denotes |
whereas |
T4177 |
15977-15985 |
VBN |
denotes |
affected |
T4178 |
15932-15935 |
DT |
denotes |
all |
T4179 |
15957-15967 |
NNS |
denotes |
parameters |
T4180 |
15936-15941 |
JJ |
denotes |
other |
T4181 |
15942-15956 |
JJ |
denotes |
haematological |
T4182 |
15968-15972 |
VBD |
denotes |
were |
T4183 |
15973-15976 |
RB |
denotes |
not |
T4184 |
15985-15986 |
. |
denotes |
. |
T4185 |
15986-16114 |
sentence |
denotes |
The mean platelet counts from wild type and anxA7-/- mutant mice are determined as 674 × 103/μl and 774 × 103/μl, respectively. |
T4186 |
15987-15990 |
DT |
denotes |
The |
T4187 |
16005-16011 |
NNS |
denotes |
counts |
T4188 |
15991-15995 |
JJ |
denotes |
mean |
T4189 |
15996-16004 |
NN |
denotes |
platelet |
T4190 |
16056-16066 |
VBN |
denotes |
determined |
T4191 |
16012-16016 |
IN |
denotes |
from |
T4192 |
16017-16021 |
JJ |
denotes |
wild |
T4193 |
16022-16026 |
NN |
denotes |
type |
T4194 |
16047-16051 |
NNS |
denotes |
mice |
T4195 |
16027-16030 |
CC |
denotes |
and |
T4196 |
16031-16036 |
NN |
denotes |
anxA7 |
T4197 |
16036-16037 |
SYM |
denotes |
- |
T4198 |
16037-16038 |
HYPH |
denotes |
/ |
T4199 |
16038-16039 |
SYM |
denotes |
- |
T4200 |
16040-16046 |
JJ |
denotes |
mutant |
T4201 |
16052-16055 |
VBP |
denotes |
are |
T4202 |
16067-16069 |
IN |
denotes |
as |
T4203 |
16070-16073 |
CD |
denotes |
674 |
T4204 |
16076-16079 |
CD |
denotes |
103 |
T4205 |
16074-16075 |
SYM |
denotes |
× |
T4206 |
16079-16080 |
SYM |
denotes |
/ |
T4207 |
16080-16082 |
NNS |
denotes |
μl |
T4208 |
16083-16086 |
CC |
denotes |
and |
T4209 |
16087-16090 |
CD |
denotes |
774 |
T4210 |
16093-16096 |
CD |
denotes |
103 |
T4211 |
16091-16092 |
SYM |
denotes |
× |
T4212 |
16096-16097 |
SYM |
denotes |
/ |
T4213 |
16097-16099 |
NNS |
denotes |
μl |
T4214 |
16099-16101 |
, |
denotes |
, |
T4215 |
16101-16113 |
RB |
denotes |
respectively |
T4216 |
16113-16114 |
. |
denotes |
. |
T4217 |
16114-16227 |
sentence |
denotes |
The platelet counts in knock out mice are significantly higher (p = 0.0275; n knock out = 11, n wild type = 14). |
T4218 |
16115-16118 |
DT |
denotes |
The |
T4219 |
16128-16134 |
NNS |
denotes |
counts |
T4220 |
16119-16127 |
NN |
denotes |
platelet |
T4221 |
16153-16156 |
VBP |
denotes |
are |
T4222 |
16135-16137 |
IN |
denotes |
in |
T4223 |
16138-16143 |
NN |
denotes |
knock |
T4224 |
16144-16147 |
NN |
denotes |
out |
T4225 |
16148-16152 |
NNS |
denotes |
mice |
T4226 |
16157-16170 |
RB |
denotes |
significantly |
T4227 |
16171-16177 |
JJR |
denotes |
higher |
T4228 |
16178-16179 |
-LRB- |
denotes |
( |
T4229 |
16223-16225 |
CD |
denotes |
14 |
T4230 |
16179-16180 |
NN |
denotes |
p |
T4231 |
16183-16189 |
CD |
denotes |
0.0275 |
T4232 |
16181-16182 |
SYM |
denotes |
= |
T4233 |
16189-16190 |
: |
denotes |
; |
T4234 |
16191-16192 |
NN |
denotes |
n |
T4235 |
16199-16202 |
NN |
denotes |
out |
T4236 |
16193-16198 |
NN |
denotes |
knock |
T4237 |
16205-16207 |
CD |
denotes |
11 |
T4238 |
16203-16204 |
SYM |
denotes |
= |
T4239 |
16207-16209 |
, |
denotes |
, |
T4240 |
16209-16210 |
NN |
denotes |
n |
T4241 |
16216-16220 |
NN |
denotes |
type |
T4242 |
16211-16215 |
JJ |
denotes |
wild |
T4243 |
16221-16222 |
SYM |
denotes |
= |
T4244 |
16225-16226 |
-RRB- |
denotes |
) |
T4245 |
16226-16227 |
. |
denotes |
. |
T4246 |
16227-16289 |
sentence |
denotes |
An increase in platelet counts is a rather uncommon disorder. |
T4247 |
16228-16230 |
DT |
denotes |
An |
T4248 |
16231-16239 |
NN |
denotes |
increase |
T4249 |
16259-16261 |
VBZ |
denotes |
is |
T4250 |
16240-16242 |
IN |
denotes |
in |
T4251 |
16243-16251 |
NN |
denotes |
platelet |
T4252 |
16252-16258 |
NNS |
denotes |
counts |
T4253 |
16262-16263 |
DT |
denotes |
a |
T4254 |
16280-16288 |
NN |
denotes |
disorder |
T4255 |
16264-16270 |
RB |
denotes |
rather |
T4256 |
16271-16279 |
JJ |
denotes |
uncommon |
T4257 |
16288-16289 |
. |
denotes |
. |
T4258 |
16289-16465 |
sentence |
denotes |
In humans, platelet counts normally increase only transiently as in reactive thrombocytosis and under postoperative conditions or are largely increased in neoplastic diseases. |
T4259 |
16290-16292 |
IN |
denotes |
In |
T4260 |
16326-16334 |
VBP |
denotes |
increase |
T4261 |
16293-16299 |
NNS |
denotes |
humans |
T4262 |
16299-16301 |
, |
denotes |
, |
T4263 |
16301-16309 |
NN |
denotes |
platelet |
T4264 |
16310-16316 |
NNS |
denotes |
counts |
T4265 |
16317-16325 |
RB |
denotes |
normally |
T4266 |
16335-16339 |
RB |
denotes |
only |
T4267 |
16340-16351 |
RB |
denotes |
transiently |
T4268 |
16352-16354 |
IN |
denotes |
as |
T4269 |
16355-16357 |
IN |
denotes |
in |
T4270 |
16358-16366 |
JJ |
denotes |
reactive |
T4271 |
16367-16381 |
NN |
denotes |
thrombocytosis |
T4272 |
16382-16385 |
CC |
denotes |
and |
T4273 |
16386-16391 |
IN |
denotes |
under |
T4274 |
16392-16405 |
JJ |
denotes |
postoperative |
T4275 |
16406-16416 |
NNS |
denotes |
conditions |
T4276 |
16417-16419 |
CC |
denotes |
or |
T4277 |
16420-16423 |
VBP |
denotes |
are |
T4278 |
16432-16441 |
VBN |
denotes |
increased |
T4279 |
16424-16431 |
RB |
denotes |
largely |
T4280 |
16442-16444 |
IN |
denotes |
in |
T4281 |
16445-16455 |
JJ |
denotes |
neoplastic |
T4282 |
16456-16464 |
NNS |
denotes |
diseases |
T4283 |
16464-16465 |
. |
denotes |
. |
T4284 |
16465-16519 |
sentence |
denotes |
By contrast, the anxA7-/-mice are healthy and viable. |
T4285 |
16466-16468 |
IN |
denotes |
By |
T4286 |
16496-16499 |
VBP |
denotes |
are |
T4287 |
16469-16477 |
NN |
denotes |
contrast |
T4288 |
16477-16479 |
, |
denotes |
, |
T4289 |
16479-16482 |
DT |
denotes |
the |
T4290 |
16491-16495 |
NNS |
denotes |
mice |
T4291 |
16483-16488 |
NN |
denotes |
anxA7 |
T4292 |
16488-16489 |
SYM |
denotes |
- |
T4293 |
16489-16490 |
HYPH |
denotes |
/ |
T4294 |
16490-16491 |
SYM |
denotes |
- |
T4295 |
16500-16507 |
JJ |
denotes |
healthy |
T4296 |
16508-16511 |
CC |
denotes |
and |
T4297 |
16512-16518 |
JJ |
denotes |
viable |
T4298 |
16518-16519 |
. |
denotes |
. |
T4299 |
16519-16628 |
sentence |
denotes |
Therefore we tested the platelet function and performed aggregometry measurements with platelet rich plasma. |
T4300 |
16520-16529 |
RB |
denotes |
Therefore |
T4301 |
16533-16539 |
VBD |
denotes |
tested |
T4302 |
16530-16532 |
PRP |
denotes |
we |
T4303 |
16540-16543 |
DT |
denotes |
the |
T4304 |
16553-16561 |
NN |
denotes |
function |
T4305 |
16544-16552 |
NN |
denotes |
platelet |
T4306 |
16562-16565 |
CC |
denotes |
and |
T4307 |
16566-16575 |
VBD |
denotes |
performed |
T4308 |
16576-16588 |
NN |
denotes |
aggregometry |
T4309 |
16589-16601 |
NNS |
denotes |
measurements |
T4310 |
16602-16606 |
IN |
denotes |
with |
T4311 |
16607-16615 |
NN |
denotes |
platelet |
T4312 |
16616-16620 |
JJ |
denotes |
rich |
T4313 |
16621-16627 |
NN |
denotes |
plasma |
T4314 |
16627-16628 |
. |
denotes |
. |
T4315 |
16628-16763 |
sentence |
denotes |
Platelet activation is observed as a morphological change from the resting discoid state to activated spherical cells with pseudopods. |
T4316 |
16629-16637 |
NN |
denotes |
Platelet |
T4317 |
16638-16648 |
NN |
denotes |
activation |
T4318 |
16652-16660 |
VBN |
denotes |
observed |
T4319 |
16649-16651 |
VBZ |
denotes |
is |
T4320 |
16661-16663 |
IN |
denotes |
as |
T4321 |
16664-16665 |
DT |
denotes |
a |
T4322 |
16680-16686 |
NN |
denotes |
change |
T4323 |
16666-16679 |
JJ |
denotes |
morphological |
T4324 |
16687-16691 |
IN |
denotes |
from |
T4325 |
16692-16695 |
DT |
denotes |
the |
T4326 |
16712-16717 |
NN |
denotes |
state |
T4327 |
16696-16703 |
VBG |
denotes |
resting |
T4328 |
16704-16711 |
JJ |
denotes |
discoid |
T4329 |
16718-16720 |
IN |
denotes |
to |
T4330 |
16721-16730 |
VBN |
denotes |
activated |
T4331 |
16741-16746 |
NNS |
denotes |
cells |
T4332 |
16731-16740 |
JJ |
denotes |
spherical |
T4333 |
16747-16751 |
IN |
denotes |
with |
T4334 |
16752-16762 |
NNS |
denotes |
pseudopods |
T4335 |
16762-16763 |
. |
denotes |
. |
T4336 |
16763-16829 |
sentence |
denotes |
The morphological changes are due to cytoskeletal rearrangements. |
T4337 |
16764-16767 |
DT |
denotes |
The |
T4338 |
16782-16789 |
NNS |
denotes |
changes |
T4339 |
16768-16781 |
JJ |
denotes |
morphological |
T4340 |
16790-16793 |
VBP |
denotes |
are |
T4341 |
16794-16797 |
IN |
denotes |
due |
T4342 |
16798-16800 |
IN |
denotes |
to |
T4343 |
16801-16813 |
JJ |
denotes |
cytoskeletal |
T4344 |
16814-16828 |
NNS |
denotes |
rearrangements |
T4345 |
16828-16829 |
. |
denotes |
. |
T4346 |
16829-16951 |
sentence |
denotes |
In vitro the activated cells form aggregates recruiting additional cells in the solution thereby reducing its cloudiness. |
T4347 |
16830-16832 |
FW |
denotes |
In |
T4348 |
16833-16838 |
FW |
denotes |
vitro |
T4349 |
16859-16863 |
VBP |
denotes |
form |
T4350 |
16839-16842 |
DT |
denotes |
the |
T4351 |
16853-16858 |
NNS |
denotes |
cells |
T4352 |
16843-16852 |
VBN |
denotes |
activated |
T4353 |
16864-16874 |
NNS |
denotes |
aggregates |
T4354 |
16875-16885 |
VBG |
denotes |
recruiting |
T4355 |
16886-16896 |
JJ |
denotes |
additional |
T4356 |
16897-16902 |
NNS |
denotes |
cells |
T4357 |
16903-16905 |
IN |
denotes |
in |
T4358 |
16906-16909 |
DT |
denotes |
the |
T4359 |
16910-16918 |
NN |
denotes |
solution |
T4360 |
16919-16926 |
RB |
denotes |
thereby |
T4361 |
16927-16935 |
VBG |
denotes |
reducing |
T4362 |
16936-16939 |
PRP$ |
denotes |
its |
T4363 |
16940-16950 |
NN |
denotes |
cloudiness |
T4364 |
16950-16951 |
. |
denotes |
. |
T4365 |
16951-17250 |
sentence |
denotes |
Analysis of the transmission values of aggregation curves after platelet activation by ristocetin addition (von Willebrand cofactor) showed that both curves differed significantly at the time point of seven seconds after platelet initiation (p = 0.0287, n knock out = 16, n wild type = 15; Fig. 6). |
T4366 |
16952-16960 |
NN |
denotes |
Analysis |
T4367 |
17085-17091 |
VBD |
denotes |
showed |
T4368 |
16961-16963 |
IN |
denotes |
of |
T4369 |
16964-16967 |
DT |
denotes |
the |
T4370 |
16981-16987 |
NNS |
denotes |
values |
T4371 |
16968-16980 |
NN |
denotes |
transmission |
T4372 |
16988-16990 |
IN |
denotes |
of |
T4373 |
16991-17002 |
NN |
denotes |
aggregation |
T4374 |
17003-17009 |
NNS |
denotes |
curves |
T4375 |
17010-17015 |
IN |
denotes |
after |
T4376 |
17016-17024 |
NN |
denotes |
platelet |
T4377 |
17025-17035 |
NN |
denotes |
activation |
T4378 |
17036-17038 |
IN |
denotes |
by |
T4379 |
17039-17049 |
NN |
denotes |
ristocetin |
T4380 |
17050-17058 |
NN |
denotes |
addition |
T4381 |
17059-17060 |
-LRB- |
denotes |
( |
T4382 |
17075-17083 |
NN |
denotes |
cofactor |
T4383 |
17060-17063 |
NN |
denotes |
von |
T4384 |
17064-17074 |
NN |
denotes |
Willebrand |
T4385 |
17083-17084 |
-RRB- |
denotes |
) |
T4386 |
17092-17096 |
IN |
denotes |
that |
T4387 |
17109-17117 |
VBD |
denotes |
differed |
T4388 |
17097-17101 |
DT |
denotes |
both |
T4389 |
17102-17108 |
NNS |
denotes |
curves |
T4390 |
17118-17131 |
RB |
denotes |
significantly |
T4391 |
17132-17134 |
IN |
denotes |
at |
T4392 |
17135-17138 |
DT |
denotes |
the |
T4393 |
17144-17149 |
NN |
denotes |
point |
T4394 |
17139-17143 |
NN |
denotes |
time |
T4395 |
17150-17152 |
IN |
denotes |
of |
T4396 |
17153-17158 |
CD |
denotes |
seven |
T4397 |
17159-17166 |
NNS |
denotes |
seconds |
T4398 |
17167-17172 |
IN |
denotes |
after |
T4399 |
17173-17181 |
NN |
denotes |
platelet |
T4400 |
17182-17192 |
NN |
denotes |
initiation |
T4401 |
17193-17194 |
-LRB- |
denotes |
( |
T4402 |
17242-17246 |
NN |
denotes |
Fig. |
T4403 |
17194-17195 |
NN |
denotes |
p |
T4404 |
17198-17204 |
CD |
denotes |
0.0287 |
T4405 |
17196-17197 |
SYM |
denotes |
= |
T4406 |
17204-17206 |
, |
denotes |
, |
T4407 |
17206-17207 |
NN |
denotes |
n |
T4408 |
17214-17217 |
NN |
denotes |
out |
T4409 |
17208-17213 |
NN |
denotes |
knock |
T4410 |
17220-17222 |
CD |
denotes |
16 |
T4411 |
17218-17219 |
SYM |
denotes |
= |
T4412 |
17222-17224 |
, |
denotes |
, |
T4413 |
17224-17225 |
NN |
denotes |
n |
T4414 |
17231-17235 |
NN |
denotes |
type |
T4415 |
17226-17230 |
JJ |
denotes |
wild |
T4416 |
17238-17240 |
CD |
denotes |
15 |
T4417 |
17236-17237 |
SYM |
denotes |
= |
T4418 |
17240-17241 |
: |
denotes |
; |
T4419 |
17247-17248 |
CD |
denotes |
6 |
T4420 |
17248-17249 |
-RRB- |
denotes |
) |
T4421 |
17249-17250 |
. |
denotes |
. |
T4422 |
17250-17329 |
sentence |
denotes |
Annexin A7 deficient platelets showed a slightly lowered aggregation velocity. |
T4423 |
17251-17258 |
NN |
denotes |
Annexin |
T4424 |
17259-17261 |
NN |
denotes |
A7 |
T4425 |
17262-17271 |
JJ |
denotes |
deficient |
T4426 |
17272-17281 |
NNS |
denotes |
platelets |
T4427 |
17282-17288 |
VBD |
denotes |
showed |
T4428 |
17289-17290 |
DT |
denotes |
a |
T4429 |
17320-17328 |
NN |
denotes |
velocity |
T4430 |
17291-17299 |
RB |
denotes |
slightly |
T4431 |
17300-17307 |
VBN |
denotes |
lowered |
T4432 |
17308-17319 |
NN |
denotes |
aggregation |
T4433 |
17328-17329 |
. |
denotes |
. |
T4434 |
17329-17640 |
sentence |
denotes |
When we compared the aggregation curves of human platelets from a healthy donor with the ones obtained from an individual with a von Willebrand factor type 1 defect, we found that the difference in the curves was much more pronounced as observed in our studies of healthy mouse platelets and anxA7-/-platelets. |
T4435 |
17330-17334 |
WRB |
denotes |
When |
T4436 |
17338-17346 |
VBD |
denotes |
compared |
T4437 |
17335-17337 |
PRP |
denotes |
we |
T4438 |
17499-17504 |
VBD |
denotes |
found |
T4439 |
17347-17350 |
DT |
denotes |
the |
T4440 |
17363-17369 |
NNS |
denotes |
curves |
T4441 |
17351-17362 |
NN |
denotes |
aggregation |
T4442 |
17370-17372 |
IN |
denotes |
of |
T4443 |
17373-17378 |
JJ |
denotes |
human |
T4444 |
17379-17388 |
NNS |
denotes |
platelets |
T4445 |
17389-17393 |
IN |
denotes |
from |
T4446 |
17394-17395 |
DT |
denotes |
a |
T4447 |
17404-17409 |
NN |
denotes |
donor |
T4448 |
17396-17403 |
JJ |
denotes |
healthy |
T4449 |
17410-17414 |
IN |
denotes |
with |
T4450 |
17415-17418 |
DT |
denotes |
the |
T4451 |
17419-17423 |
NNS |
denotes |
ones |
T4452 |
17424-17432 |
VBN |
denotes |
obtained |
T4453 |
17433-17437 |
IN |
denotes |
from |
T4454 |
17438-17440 |
DT |
denotes |
an |
T4455 |
17441-17451 |
NN |
denotes |
individual |
T4456 |
17452-17456 |
IN |
denotes |
with |
T4457 |
17457-17458 |
DT |
denotes |
a |
T4458 |
17488-17494 |
NN |
denotes |
defect |
T4459 |
17459-17462 |
NN |
denotes |
von |
T4460 |
17463-17473 |
NN |
denotes |
Willebrand |
T4461 |
17474-17480 |
NN |
denotes |
factor |
T4462 |
17481-17485 |
NN |
denotes |
type |
T4463 |
17486-17487 |
CD |
denotes |
1 |
T4464 |
17494-17496 |
, |
denotes |
, |
T4465 |
17496-17498 |
PRP |
denotes |
we |
T4466 |
17505-17509 |
IN |
denotes |
that |
T4467 |
17539-17542 |
VBD |
denotes |
was |
T4468 |
17510-17513 |
DT |
denotes |
the |
T4469 |
17514-17524 |
NN |
denotes |
difference |
T4470 |
17525-17527 |
IN |
denotes |
in |
T4471 |
17528-17531 |
DT |
denotes |
the |
T4472 |
17532-17538 |
NNS |
denotes |
curves |
T4473 |
17543-17547 |
RB |
denotes |
much |
T4474 |
17548-17552 |
RBR |
denotes |
more |
T4475 |
17553-17563 |
JJ |
denotes |
pronounced |
T4476 |
17564-17566 |
IN |
denotes |
as |
T4477 |
17567-17575 |
VBN |
denotes |
observed |
T4478 |
17576-17578 |
IN |
denotes |
in |
T4479 |
17579-17582 |
PRP$ |
denotes |
our |
T4480 |
17583-17590 |
NNS |
denotes |
studies |
T4481 |
17591-17593 |
IN |
denotes |
of |
T4482 |
17594-17601 |
JJ |
denotes |
healthy |
T4483 |
17608-17617 |
NNS |
denotes |
platelets |
T4484 |
17602-17607 |
NN |
denotes |
mouse |
T4485 |
17618-17621 |
CC |
denotes |
and |
T4486 |
17622-17627 |
NN |
denotes |
anxA7 |
T4487 |
17630-17639 |
NNS |
denotes |
platelets |
T4488 |
17627-17628 |
SYM |
denotes |
- |
T4489 |
17628-17629 |
HYPH |
denotes |
/ |
T4490 |
17629-17630 |
SYM |
denotes |
- |
T4491 |
17639-17640 |
. |
denotes |
. |
T4492 |
17640-17779 |
sentence |
denotes |
Furthermore we found that murine platelets responded immediately to the initiating chemical whereas human platelets have a lag phase [23]. |
T4493 |
17641-17652 |
RB |
denotes |
Furthermore |
T4494 |
17656-17661 |
VBD |
denotes |
found |
T4495 |
17653-17655 |
PRP |
denotes |
we |
T4496 |
17662-17666 |
IN |
denotes |
that |
T4497 |
17684-17693 |
VBD |
denotes |
responded |
T4498 |
17667-17673 |
JJ |
denotes |
murine |
T4499 |
17674-17683 |
NNS |
denotes |
platelets |
T4500 |
17694-17705 |
RB |
denotes |
immediately |
T4501 |
17706-17708 |
IN |
denotes |
to |
T4502 |
17709-17712 |
DT |
denotes |
the |
T4503 |
17724-17732 |
NN |
denotes |
chemical |
T4504 |
17713-17723 |
VBG |
denotes |
initiating |
T4505 |
17733-17740 |
IN |
denotes |
whereas |
T4506 |
17757-17761 |
VBP |
denotes |
have |
T4507 |
17741-17746 |
JJ |
denotes |
human |
T4508 |
17747-17756 |
NNS |
denotes |
platelets |
T4509 |
17762-17763 |
DT |
denotes |
a |
T4510 |
17768-17773 |
NN |
denotes |
phase |
T4511 |
17764-17767 |
NN |
denotes |
lag |
T4512 |
17774-17775 |
-LRB- |
denotes |
[ |
T4513 |
17775-17777 |
CD |
denotes |
23 |
T4514 |
17777-17778 |
-RRB- |
denotes |
] |
T4515 |
17778-17779 |
. |
denotes |
. |
T5213 |
18874-18881 |
NN |
denotes |
Annexin |
T5214 |
18882-18884 |
NN |
denotes |
A7 |
T5215 |
18888-18895 |
VBN |
denotes |
thought |
T5216 |
18885-18887 |
VBZ |
denotes |
is |
T5217 |
18896-18898 |
TO |
denotes |
to |
T5218 |
18899-18903 |
VB |
denotes |
have |
T5219 |
18904-18905 |
DT |
denotes |
a |
T5220 |
18906-18910 |
NN |
denotes |
role |
T5221 |
18911-18913 |
IN |
denotes |
in |
T5222 |
18914-18921 |
NN |
denotes |
vesicle |
T5223 |
18922-18928 |
NN |
denotes |
fusion |
T5224 |
18929-18932 |
CC |
denotes |
and |
T5225 |
18933-18935 |
IN |
denotes |
in |
T5226 |
18936-18946 |
VBG |
denotes |
regulating |
T5227 |
18947-18950 |
CC |
denotes |
and |
T5228 |
18951-18962 |
VBG |
denotes |
stabilizing |
T5229 |
18963-18971 |
NN |
denotes |
membrane |
T5230 |
18972-18979 |
NNS |
denotes |
domains |
T5231 |
18980-18981 |
-LRB- |
denotes |
[ |
T5232 |
18981-18982 |
CD |
denotes |
1 |
T5233 |
18982-18983 |
-RRB- |
denotes |
] |
T5234 |
18983-18984 |
. |
denotes |
. |
T5235 |
18984-19164 |
sentence |
denotes |
The protein has been localized to the plasma membrane including raft subdomains, furthermore it is present in the cytosol where it is found on subcellular vesicle-like structures. |
T5236 |
18985-18988 |
DT |
denotes |
The |
T5237 |
18989-18996 |
NN |
denotes |
protein |
T5238 |
19006-19015 |
VBN |
denotes |
localized |
T5239 |
18997-19000 |
VBZ |
denotes |
has |
T5240 |
19001-19005 |
VBN |
denotes |
been |
T5241 |
19081-19083 |
VBZ |
denotes |
is |
T5242 |
19016-19018 |
IN |
denotes |
to |
T5243 |
19019-19022 |
DT |
denotes |
the |
T5244 |
19030-19038 |
NN |
denotes |
membrane |
T5245 |
19023-19029 |
NN |
denotes |
plasma |
T5246 |
19039-19048 |
VBG |
denotes |
including |
T5247 |
19049-19053 |
NN |
denotes |
raft |
T5248 |
19054-19064 |
NNS |
denotes |
subdomains |
T5249 |
19064-19066 |
, |
denotes |
, |
T5250 |
19066-19077 |
RB |
denotes |
furthermore |
T5251 |
19078-19080 |
PRP |
denotes |
it |
T5252 |
19084-19091 |
JJ |
denotes |
present |
T5253 |
19092-19094 |
IN |
denotes |
in |
T5254 |
19095-19098 |
DT |
denotes |
the |
T5255 |
19099-19106 |
NN |
denotes |
cytosol |
T5256 |
19107-19112 |
WRB |
denotes |
where |
T5257 |
19119-19124 |
VBN |
denotes |
found |
T5258 |
19113-19115 |
PRP |
denotes |
it |
T5259 |
19116-19118 |
VBZ |
denotes |
is |
T5260 |
19125-19127 |
IN |
denotes |
on |
T5261 |
19128-19139 |
JJ |
denotes |
subcellular |
T5262 |
19153-19163 |
NNS |
denotes |
structures |
T5263 |
19140-19147 |
NN |
denotes |
vesicle |
T5264 |
19148-19152 |
JJ |
denotes |
like |
T5265 |
19147-19148 |
HYPH |
denotes |
- |
T5266 |
19163-19164 |
. |
denotes |
. |
T5267 |
19164-19245 |
sentence |
denotes |
This distribution appears to be independent of the tissue or cell type analysed. |
T5268 |
19165-19169 |
DT |
denotes |
This |
T5269 |
19170-19182 |
NN |
denotes |
distribution |
T5270 |
19183-19190 |
VBZ |
denotes |
appears |
T5271 |
19191-19193 |
TO |
denotes |
to |
T5272 |
19194-19196 |
VB |
denotes |
be |
T5273 |
19197-19208 |
JJ |
denotes |
independent |
T5274 |
19209-19211 |
IN |
denotes |
of |
T5275 |
19212-19215 |
DT |
denotes |
the |
T5276 |
19231-19235 |
NN |
denotes |
type |
T5277 |
19216-19222 |
NN |
denotes |
tissue |
T5278 |
19223-19225 |
CC |
denotes |
or |
T5279 |
19226-19230 |
NN |
denotes |
cell |
T5280 |
19236-19244 |
VBN |
denotes |
analysed |
T5281 |
19244-19245 |
. |
denotes |
. |
T5282 |
19245-19399 |
sentence |
denotes |
In specialized or terminally differentiated cells, expression levels increase and frequently the larger of the two isoforms is being expressed [6,34,35]. |
T5283 |
19246-19248 |
IN |
denotes |
In |
T5284 |
19315-19323 |
VBP |
denotes |
increase |
T5285 |
19249-19260 |
VBN |
denotes |
specialized |
T5286 |
19290-19295 |
NNS |
denotes |
cells |
T5287 |
19261-19263 |
CC |
denotes |
or |
T5288 |
19264-19274 |
RB |
denotes |
terminally |
T5289 |
19275-19289 |
VBN |
denotes |
differentiated |
T5290 |
19295-19297 |
, |
denotes |
, |
T5291 |
19297-19307 |
NN |
denotes |
expression |
T5292 |
19308-19314 |
NNS |
denotes |
levels |
T5293 |
19324-19327 |
CC |
denotes |
and |
T5294 |
19328-19338 |
RB |
denotes |
frequently |
T5295 |
19379-19388 |
VBN |
denotes |
expressed |
T5296 |
19339-19342 |
DT |
denotes |
the |
T5297 |
19343-19349 |
JJR |
denotes |
larger |
T5298 |
19350-19352 |
IN |
denotes |
of |
T5299 |
19353-19356 |
DT |
denotes |
the |
T5300 |
19361-19369 |
NNS |
denotes |
isoforms |
T5301 |
19357-19360 |
CD |
denotes |
two |
T5302 |
19370-19372 |
VBZ |
denotes |
is |
T5303 |
19373-19378 |
VBG |
denotes |
being |
T5304 |
19389-19390 |
-LRB- |
denotes |
[ |
T5305 |
19395-19397 |
CD |
denotes |
35 |
T5306 |
19390-19391 |
CD |
denotes |
6 |
T5307 |
19391-19392 |
, |
denotes |
, |
T5308 |
19392-19394 |
CD |
denotes |
34 |
T5309 |
19394-19395 |
, |
denotes |
, |
T5310 |
19397-19398 |
-RRB- |
denotes |
] |
T5311 |
19398-19399 |
. |
denotes |
. |
T5312 |
19399-19558 |
sentence |
denotes |
Red blood cells, which were thought to be devoid of annexin A7, were recently shown to harbor the 47 kDa isoform together with its binding partner sorcin [7]. |
T5313 |
19400-19403 |
JJ |
denotes |
Red |
T5314 |
19410-19415 |
NNS |
denotes |
cells |
T5315 |
19404-19409 |
NN |
denotes |
blood |
T5316 |
19478-19483 |
VBN |
denotes |
shown |
T5317 |
19415-19417 |
, |
denotes |
, |
T5318 |
19417-19422 |
WDT |
denotes |
which |
T5319 |
19428-19435 |
VBN |
denotes |
thought |
T5320 |
19423-19427 |
VBD |
denotes |
were |
T5321 |
19436-19438 |
TO |
denotes |
to |
T5322 |
19439-19441 |
VB |
denotes |
be |
T5323 |
19442-19448 |
JJ |
denotes |
devoid |
T5324 |
19449-19451 |
IN |
denotes |
of |
T5325 |
19452-19459 |
NN |
denotes |
annexin |
T5326 |
19460-19462 |
NN |
denotes |
A7 |
T5327 |
19462-19464 |
, |
denotes |
, |
T5328 |
19464-19468 |
VBD |
denotes |
were |
T5329 |
19469-19477 |
RB |
denotes |
recently |
T5330 |
19484-19486 |
TO |
denotes |
to |
T5331 |
19487-19493 |
VB |
denotes |
harbor |
T5332 |
19494-19497 |
DT |
denotes |
the |
T5333 |
19505-19512 |
NN |
denotes |
isoform |
T5334 |
19498-19500 |
CD |
denotes |
47 |
T5335 |
19501-19504 |
NN |
denotes |
kDa |
T5336 |
19513-19521 |
RB |
denotes |
together |
T5337 |
19522-19526 |
IN |
denotes |
with |
T5338 |
19527-19530 |
PRP$ |
denotes |
its |
T5339 |
19539-19546 |
NN |
denotes |
partner |
T5340 |
19531-19538 |
VBG |
denotes |
binding |
T5341 |
19547-19553 |
NN |
denotes |
sorcin |
T5342 |
19554-19555 |
-LRB- |
denotes |
[ |
T5343 |
19555-19556 |
CD |
denotes |
7 |
T5344 |
19556-19557 |
-RRB- |
denotes |
] |
T5345 |
19557-19558 |
. |
denotes |
. |
T5346 |
19558-19682 |
sentence |
denotes |
We showed the presence of the 51 kDa isoform as well, adding red blood cells to the list of cells that harbor this isoform. |
T5347 |
19559-19561 |
PRP |
denotes |
We |
T5348 |
19562-19568 |
VBD |
denotes |
showed |
T5349 |
19569-19572 |
DT |
denotes |
the |
T5350 |
19573-19581 |
NN |
denotes |
presence |
T5351 |
19582-19584 |
IN |
denotes |
of |
T5352 |
19585-19588 |
DT |
denotes |
the |
T5353 |
19596-19603 |
NN |
denotes |
isoform |
T5354 |
19589-19591 |
CD |
denotes |
51 |
T5355 |
19592-19595 |
NN |
denotes |
kDa |
T5356 |
19604-19606 |
RB |
denotes |
as |
T5357 |
19607-19611 |
RB |
denotes |
well |
T5358 |
19611-19613 |
, |
denotes |
, |
T5359 |
19613-19619 |
VBG |
denotes |
adding |
T5360 |
19620-19623 |
JJ |
denotes |
red |
T5361 |
19630-19635 |
NNS |
denotes |
cells |
T5362 |
19624-19629 |
NN |
denotes |
blood |
T5363 |
19636-19638 |
IN |
denotes |
to |
T5364 |
19639-19642 |
DT |
denotes |
the |
T5365 |
19643-19647 |
NN |
denotes |
list |
T5366 |
19648-19650 |
IN |
denotes |
of |
T5367 |
19651-19656 |
NNS |
denotes |
cells |
T5368 |
19657-19661 |
WDT |
denotes |
that |
T5369 |
19662-19668 |
VBP |
denotes |
harbor |
T5370 |
19669-19673 |
DT |
denotes |
this |
T5371 |
19674-19681 |
NN |
denotes |
isoform |
T5372 |
19681-19682 |
. |
denotes |
. |
T5373 |
19682-19906 |
sentence |
denotes |
Salzer et al. [7] not only demonstrated the presence of annexin A7 in red blood cells, they also showed that annexin A7 and sorcin were enriched in membrane raft domains of nanovesicles formed from red blood cells in vitro. |
T5374 |
19683-19689 |
NNP |
denotes |
Salzer |
T5375 |
19780-19786 |
VBD |
denotes |
showed |
T5376 |
19690-19692 |
FW |
denotes |
et |
T5377 |
19693-19696 |
FW |
denotes |
al. |
T5378 |
19697-19698 |
-LRB- |
denotes |
[ |
T5379 |
19698-19699 |
CD |
denotes |
7 |
T5380 |
19699-19700 |
-RRB- |
denotes |
] |
T5381 |
19701-19704 |
RB |
denotes |
not |
T5382 |
19705-19709 |
RB |
denotes |
only |
T5383 |
19710-19722 |
VBD |
denotes |
demonstrated |
T5384 |
19723-19726 |
DT |
denotes |
the |
T5385 |
19727-19735 |
NN |
denotes |
presence |
T5386 |
19736-19738 |
IN |
denotes |
of |
T5387 |
19739-19746 |
NN |
denotes |
annexin |
T5388 |
19747-19749 |
NN |
denotes |
A7 |
T5389 |
19750-19752 |
IN |
denotes |
in |
T5390 |
19753-19756 |
JJ |
denotes |
red |
T5391 |
19763-19768 |
NNS |
denotes |
cells |
T5392 |
19757-19762 |
NN |
denotes |
blood |
T5393 |
19768-19770 |
, |
denotes |
, |
T5394 |
19770-19774 |
PRP |
denotes |
they |
T5395 |
19775-19779 |
RB |
denotes |
also |
T5396 |
19787-19791 |
IN |
denotes |
that |
T5397 |
19814-19818 |
VBD |
denotes |
were |
T5398 |
19792-19799 |
NN |
denotes |
annexin |
T5399 |
19800-19802 |
NN |
denotes |
A7 |
T5400 |
19803-19806 |
CC |
denotes |
and |
T5401 |
19807-19813 |
NN |
denotes |
sorcin |
T5402 |
19819-19827 |
JJ |
denotes |
enriched |
T5403 |
19828-19830 |
IN |
denotes |
in |
T5404 |
19831-19839 |
NN |
denotes |
membrane |
T5405 |
19840-19844 |
NN |
denotes |
raft |
T5406 |
19845-19852 |
NNS |
denotes |
domains |
T5407 |
19853-19855 |
IN |
denotes |
of |
T5408 |
19856-19868 |
NNS |
denotes |
nanovesicles |
T5409 |
19869-19875 |
VBN |
denotes |
formed |
T5410 |
19876-19880 |
IN |
denotes |
from |
T5411 |
19881-19884 |
JJ |
denotes |
red |
T5412 |
19891-19896 |
NNS |
denotes |
cells |
T5413 |
19885-19890 |
NN |
denotes |
blood |
T5414 |
19897-19899 |
FW |
denotes |
in |
T5415 |
19900-19905 |
FW |
denotes |
vitro |
T5416 |
19905-19906 |
. |
denotes |
. |
T5417 |
19906-20025 |
sentence |
denotes |
Raft domains in general are considered key players in membrane organization and in mediating the vesiculation process. |
T5418 |
19907-19911 |
NN |
denotes |
Raft |
T5419 |
19912-19919 |
NNS |
denotes |
domains |
T5420 |
19935-19945 |
VBN |
denotes |
considered |
T5421 |
19920-19922 |
IN |
denotes |
in |
T5422 |
19923-19930 |
JJ |
denotes |
general |
T5423 |
19931-19934 |
VBP |
denotes |
are |
T5424 |
19946-19949 |
JJ |
denotes |
key |
T5425 |
19950-19957 |
NNS |
denotes |
players |
T5426 |
19958-19960 |
IN |
denotes |
in |
T5427 |
19961-19969 |
NN |
denotes |
membrane |
T5428 |
19970-19982 |
NN |
denotes |
organization |
T5429 |
19983-19986 |
CC |
denotes |
and |
T5430 |
19987-19989 |
IN |
denotes |
in |
T5431 |
19990-19999 |
VBG |
denotes |
mediating |
T5432 |
20000-20003 |
DT |
denotes |
the |
T5433 |
20017-20024 |
NN |
denotes |
process |
T5434 |
20004-20016 |
NN |
denotes |
vesiculation |
T5435 |
20024-20025 |
. |
denotes |
. |
T5436 |
20025-20229 |
sentence |
denotes |
They result from the clustering of sphingolipids and cholesterol in the outer leaflet of the membrane connected to phospholipids and cholesterol in the inner leaflet and are enriched in special proteins. |
T5437 |
20026-20030 |
PRP |
denotes |
They |
T5438 |
20031-20037 |
VBP |
denotes |
result |
T5439 |
20038-20042 |
IN |
denotes |
from |
T5440 |
20043-20046 |
DT |
denotes |
the |
T5441 |
20047-20057 |
NN |
denotes |
clustering |
T5442 |
20058-20060 |
IN |
denotes |
of |
T5443 |
20061-20074 |
NNS |
denotes |
sphingolipids |
T5444 |
20075-20078 |
CC |
denotes |
and |
T5445 |
20079-20090 |
NN |
denotes |
cholesterol |
T5446 |
20091-20093 |
IN |
denotes |
in |
T5447 |
20094-20097 |
DT |
denotes |
the |
T5448 |
20104-20111 |
NN |
denotes |
leaflet |
T5449 |
20098-20103 |
JJ |
denotes |
outer |
T5450 |
20112-20114 |
IN |
denotes |
of |
T5451 |
20115-20118 |
DT |
denotes |
the |
T5452 |
20119-20127 |
NN |
denotes |
membrane |
T5453 |
20128-20137 |
VBN |
denotes |
connected |
T5454 |
20138-20140 |
IN |
denotes |
to |
T5455 |
20141-20154 |
NNS |
denotes |
phospholipids |
T5456 |
20155-20158 |
CC |
denotes |
and |
T5457 |
20159-20170 |
NN |
denotes |
cholesterol |
T5458 |
20171-20173 |
IN |
denotes |
in |
T5459 |
20174-20177 |
DT |
denotes |
the |
T5460 |
20184-20191 |
NN |
denotes |
leaflet |
T5461 |
20178-20183 |
JJ |
denotes |
inner |
T5462 |
20192-20195 |
CC |
denotes |
and |
T5463 |
20196-20199 |
VBP |
denotes |
are |
T5464 |
20200-20208 |
JJ |
denotes |
enriched |
T5465 |
20209-20211 |
IN |
denotes |
in |
T5466 |
20212-20219 |
JJ |
denotes |
special |
T5467 |
20220-20228 |
NN |
denotes |
proteins |
T5468 |
20228-20229 |
. |
denotes |
. |
T5469 |
20229-20405 |
sentence |
denotes |
Biochemically rafts are defined as membranes that are resistant to extraction by cold Triton X-100 and can be floated to low densities in sucrose gradient centrifugation [36]. |
T5470 |
20230-20243 |
RB |
denotes |
Biochemically |
T5471 |
20254-20261 |
VBN |
denotes |
defined |
T5472 |
20244-20249 |
NNS |
denotes |
rafts |
T5473 |
20250-20253 |
VBP |
denotes |
are |
T5474 |
20262-20264 |
IN |
denotes |
as |
T5475 |
20265-20274 |
NNS |
denotes |
membranes |
T5476 |
20275-20279 |
WDT |
denotes |
that |
T5477 |
20280-20283 |
VBP |
denotes |
are |
T5478 |
20284-20293 |
JJ |
denotes |
resistant |
T5479 |
20294-20296 |
IN |
denotes |
to |
T5480 |
20297-20307 |
NN |
denotes |
extraction |
T5481 |
20308-20310 |
IN |
denotes |
by |
T5482 |
20311-20315 |
JJ |
denotes |
cold |
T5483 |
20323-20324 |
NN |
denotes |
X |
T5484 |
20316-20322 |
NN |
denotes |
Triton |
T5485 |
20324-20325 |
HYPH |
denotes |
- |
T5486 |
20325-20328 |
CD |
denotes |
100 |
T5487 |
20329-20332 |
CC |
denotes |
and |
T5488 |
20333-20336 |
MD |
denotes |
can |
T5489 |
20340-20347 |
VBN |
denotes |
floated |
T5490 |
20337-20339 |
VB |
denotes |
be |
T5491 |
20348-20350 |
IN |
denotes |
to |
T5492 |
20351-20354 |
JJ |
denotes |
low |
T5493 |
20355-20364 |
NNS |
denotes |
densities |
T5494 |
20365-20367 |
IN |
denotes |
in |
T5495 |
20368-20375 |
NN |
denotes |
sucrose |
T5496 |
20376-20384 |
NN |
denotes |
gradient |
T5497 |
20385-20399 |
NN |
denotes |
centrifugation |
T5498 |
20400-20401 |
-LRB- |
denotes |
[ |
T5499 |
20401-20403 |
CD |
denotes |
36 |
T5500 |
20403-20404 |
-RRB- |
denotes |
] |
T5501 |
20404-20405 |
. |
denotes |
. |
T5502 |
20405-20564 |
sentence |
denotes |
The findings of Caohuy et al. [37] that annexin A7 mediates membrane aggregation also suggested that annexin A7 is an essential component during vesiculation. |
T5503 |
20406-20409 |
DT |
denotes |
The |
T5504 |
20410-20418 |
NNS |
denotes |
findings |
T5505 |
20492-20501 |
VBD |
denotes |
suggested |
T5506 |
20419-20421 |
IN |
denotes |
of |
T5507 |
20422-20428 |
NNP |
denotes |
Caohuy |
T5508 |
20429-20431 |
FW |
denotes |
et |
T5509 |
20432-20435 |
FW |
denotes |
al. |
T5510 |
20436-20437 |
-LRB- |
denotes |
[ |
T5511 |
20437-20439 |
CD |
denotes |
37 |
T5512 |
20439-20440 |
-RRB- |
denotes |
] |
T5513 |
20441-20445 |
IN |
denotes |
that |
T5514 |
20457-20465 |
VBZ |
denotes |
mediates |
T5515 |
20446-20453 |
NN |
denotes |
annexin |
T5516 |
20454-20456 |
NN |
denotes |
A7 |
T5517 |
20466-20474 |
NN |
denotes |
membrane |
T5518 |
20475-20486 |
NN |
denotes |
aggregation |
T5519 |
20487-20491 |
RB |
denotes |
also |
T5520 |
20502-20506 |
IN |
denotes |
that |
T5521 |
20518-20520 |
VBZ |
denotes |
is |
T5522 |
20507-20514 |
NN |
denotes |
annexin |
T5523 |
20515-20517 |
NN |
denotes |
A7 |
T5524 |
20521-20523 |
DT |
denotes |
an |
T5525 |
20534-20543 |
NN |
denotes |
component |
T5526 |
20524-20533 |
JJ |
denotes |
essential |
T5527 |
20544-20550 |
IN |
denotes |
during |
T5528 |
20551-20563 |
NN |
denotes |
vesiculation |
T5529 |
20563-20564 |
. |
denotes |
. |
T5530 |
20564-20693 |
sentence |
denotes |
In addition they reported a further activation of the vesiculation process by GTP and proposed that annexin A7 acts as a GTPase. |
T5531 |
20565-20567 |
IN |
denotes |
In |
T5532 |
20582-20590 |
VBD |
denotes |
reported |
T5533 |
20568-20576 |
NN |
denotes |
addition |
T5534 |
20577-20581 |
PRP |
denotes |
they |
T5535 |
20591-20592 |
DT |
denotes |
a |
T5536 |
20601-20611 |
NN |
denotes |
activation |
T5537 |
20593-20600 |
JJ |
denotes |
further |
T5538 |
20612-20614 |
IN |
denotes |
of |
T5539 |
20615-20618 |
DT |
denotes |
the |
T5540 |
20632-20639 |
NN |
denotes |
process |
T5541 |
20619-20631 |
NN |
denotes |
vesiculation |
T5542 |
20640-20642 |
IN |
denotes |
by |
T5543 |
20643-20646 |
NN |
denotes |
GTP |
T5544 |
20647-20650 |
CC |
denotes |
and |
T5545 |
20651-20659 |
VBD |
denotes |
proposed |
T5546 |
20660-20664 |
IN |
denotes |
that |
T5547 |
20676-20680 |
VBZ |
denotes |
acts |
T5548 |
20665-20672 |
NN |
denotes |
annexin |
T5549 |
20673-20675 |
NN |
denotes |
A7 |
T5550 |
20681-20683 |
IN |
denotes |
as |
T5551 |
20684-20685 |
DT |
denotes |
a |
T5552 |
20686-20692 |
NN |
denotes |
GTPase |
T5553 |
20692-20693 |
. |
denotes |
. |
T5554 |
20693-20776 |
sentence |
denotes |
Similarly, the presence of GTP enhanced the secretion of surfactant by annexin A7. |
T5555 |
20694-20703 |
RB |
denotes |
Similarly |
T5556 |
20725-20733 |
VBD |
denotes |
enhanced |
T5557 |
20703-20705 |
, |
denotes |
, |
T5558 |
20705-20708 |
DT |
denotes |
the |
T5559 |
20709-20717 |
NN |
denotes |
presence |
T5560 |
20718-20720 |
IN |
denotes |
of |
T5561 |
20721-20724 |
NN |
denotes |
GTP |
T5562 |
20734-20737 |
DT |
denotes |
the |
T5563 |
20738-20747 |
NN |
denotes |
secretion |
T5564 |
20748-20750 |
IN |
denotes |
of |
T5565 |
20751-20761 |
NN |
denotes |
surfactant |
T5566 |
20762-20764 |
IN |
denotes |
by |
T5567 |
20765-20772 |
NN |
denotes |
annexin |
T5568 |
20773-20775 |
NN |
denotes |
A7 |
T5569 |
20775-20776 |
. |
denotes |
. |
T5570 |
20776-20983 |
sentence |
denotes |
These results were thought to support a direct role for annexin A7 in surfactant secretion, but in these studies annexin A7 mediated membrane fusion was separated from a second GTP-dependent mechanism [38]. |
T5571 |
20777-20782 |
DT |
denotes |
These |
T5572 |
20783-20790 |
NNS |
denotes |
results |
T5573 |
20796-20803 |
VBN |
denotes |
thought |
T5574 |
20791-20795 |
VBD |
denotes |
were |
T5575 |
20804-20806 |
TO |
denotes |
to |
T5576 |
20807-20814 |
VB |
denotes |
support |
T5577 |
20815-20816 |
DT |
denotes |
a |
T5578 |
20824-20828 |
NN |
denotes |
role |
T5579 |
20817-20823 |
JJ |
denotes |
direct |
T5580 |
20829-20832 |
IN |
denotes |
for |
T5581 |
20833-20840 |
NN |
denotes |
annexin |
T5582 |
20841-20843 |
NN |
denotes |
A7 |
T5583 |
20844-20846 |
IN |
denotes |
in |
T5584 |
20847-20857 |
JJ |
denotes |
surfactant |
T5585 |
20858-20867 |
NN |
denotes |
secretion |
T5586 |
20867-20869 |
, |
denotes |
, |
T5587 |
20869-20872 |
CC |
denotes |
but |
T5588 |
20873-20875 |
IN |
denotes |
in |
T5589 |
20930-20939 |
VBN |
denotes |
separated |
T5590 |
20876-20881 |
DT |
denotes |
these |
T5591 |
20882-20889 |
NNS |
denotes |
studies |
T5592 |
20890-20897 |
NN |
denotes |
annexin |
T5593 |
20898-20900 |
NN |
denotes |
A7 |
T5594 |
20901-20909 |
VBN |
denotes |
mediated |
T5595 |
20919-20925 |
NN |
denotes |
fusion |
T5596 |
20910-20918 |
NN |
denotes |
membrane |
T5597 |
20926-20929 |
VBD |
denotes |
was |
T5598 |
20940-20944 |
IN |
denotes |
from |
T5599 |
20945-20946 |
DT |
denotes |
a |
T5600 |
20968-20977 |
NN |
denotes |
mechanism |
T5601 |
20947-20953 |
JJ |
denotes |
second |
T5602 |
20954-20957 |
NN |
denotes |
GTP |
T5603 |
20958-20967 |
JJ |
denotes |
dependent |
T5604 |
20957-20958 |
HYPH |
denotes |
- |
T5605 |
20978-20979 |
-LRB- |
denotes |
[ |
T5606 |
20979-20981 |
CD |
denotes |
38 |
T5607 |
20981-20982 |
-RRB- |
denotes |
] |
T5608 |
20982-20983 |
. |
denotes |
. |
T5609 |
20983-21098 |
sentence |
denotes |
Having an annexin A7 knockout mouse we tested the involvement of annexin A7 in budding of red blood cell vesicles. |
T5610 |
20984-20990 |
VBG |
denotes |
Having |
T5611 |
21023-21029 |
VBD |
denotes |
tested |
T5612 |
20991-20993 |
DT |
denotes |
an |
T5613 |
21014-21019 |
NN |
denotes |
mouse |
T5614 |
20994-21001 |
NN |
denotes |
annexin |
T5615 |
21002-21004 |
NN |
denotes |
A7 |
T5616 |
21005-21013 |
NN |
denotes |
knockout |
T5617 |
21020-21022 |
PRP |
denotes |
we |
T5618 |
21030-21033 |
DT |
denotes |
the |
T5619 |
21034-21045 |
NN |
denotes |
involvement |
T5620 |
21046-21048 |
IN |
denotes |
of |
T5621 |
21049-21056 |
NN |
denotes |
annexin |
T5622 |
21057-21059 |
NN |
denotes |
A7 |
T5623 |
21060-21062 |
IN |
denotes |
in |
T5624 |
21063-21070 |
NN |
denotes |
budding |
T5625 |
21071-21073 |
IN |
denotes |
of |
T5626 |
21074-21077 |
JJ |
denotes |
red |
T5627 |
21084-21088 |
NN |
denotes |
cell |
T5628 |
21078-21083 |
NN |
denotes |
blood |
T5629 |
21089-21097 |
NNS |
denotes |
vesicles |
T5630 |
21097-21098 |
. |
denotes |
. |
T5631 |
21098-21243 |
sentence |
denotes |
Our data suggest that the efficiency of micro- and nanovesicle formation is not significantly different in wild type and mutant red blood cells. |
T5632 |
21099-21102 |
PRP$ |
denotes |
Our |
T5633 |
21103-21107 |
NNS |
denotes |
data |
T5634 |
21108-21115 |
VBP |
denotes |
suggest |
T5635 |
21116-21120 |
IN |
denotes |
that |
T5636 |
21172-21174 |
VBZ |
denotes |
is |
T5637 |
21121-21124 |
DT |
denotes |
the |
T5638 |
21125-21135 |
NN |
denotes |
efficiency |
T5639 |
21136-21138 |
IN |
denotes |
of |
T5640 |
21139-21144 |
AFX |
denotes |
micro |
T5641 |
21150-21161 |
NN |
denotes |
nanovesicle |
T5642 |
21144-21145 |
HYPH |
denotes |
- |
T5643 |
21146-21149 |
CC |
denotes |
and |
T5644 |
21162-21171 |
NN |
denotes |
formation |
T5645 |
21175-21178 |
RB |
denotes |
not |
T5646 |
21179-21192 |
RB |
denotes |
significantly |
T5647 |
21193-21202 |
JJ |
denotes |
different |
T5648 |
21203-21205 |
IN |
denotes |
in |
T5649 |
21206-21210 |
JJ |
denotes |
wild |
T5650 |
21211-21215 |
NN |
denotes |
type |
T5651 |
21237-21242 |
NNS |
denotes |
cells |
T5652 |
21216-21219 |
CC |
denotes |
and |
T5653 |
21220-21226 |
JJ |
denotes |
mutant |
T5654 |
21227-21230 |
JJ |
denotes |
red |
T5655 |
21231-21236 |
NN |
denotes |
blood |
T5656 |
21242-21243 |
. |
denotes |
. |
T5657 |
21243-21422 |
sentence |
denotes |
Likewise, Dictyostelium discoideum cells, in which the single annexin gene was inactivated, were not impaired in processes requiring membrane fusion [[39]; and data unpublished]. |
T5658 |
21244-21252 |
RB |
denotes |
Likewise |
T5659 |
21336-21340 |
VBD |
denotes |
were |
T5660 |
21252-21254 |
, |
denotes |
, |
T5661 |
21254-21267 |
NNP |
denotes |
Dictyostelium |
T5662 |
21268-21278 |
NNP |
denotes |
discoideum |
T5663 |
21279-21284 |
NNS |
denotes |
cells |
T5664 |
21284-21286 |
, |
denotes |
, |
T5665 |
21286-21288 |
IN |
denotes |
in |
T5666 |
21319-21322 |
VBD |
denotes |
was |
T5667 |
21289-21294 |
WDT |
denotes |
which |
T5668 |
21295-21298 |
DT |
denotes |
the |
T5669 |
21314-21318 |
NN |
denotes |
gene |
T5670 |
21299-21305 |
JJ |
denotes |
single |
T5671 |
21306-21313 |
NN |
denotes |
annexin |
T5672 |
21323-21334 |
JJ |
denotes |
inactivated |
T5673 |
21334-21336 |
, |
denotes |
, |
T5674 |
21341-21344 |
RB |
denotes |
not |
T5675 |
21345-21353 |
JJ |
denotes |
impaired |
T5676 |
21354-21356 |
IN |
denotes |
in |
T5677 |
21357-21366 |
NNS |
denotes |
processes |
T5678 |
21367-21376 |
VBG |
denotes |
requiring |
T5679 |
21377-21385 |
NN |
denotes |
membrane |
T5680 |
21386-21392 |
NN |
denotes |
fusion |
T5681 |
21393-21394 |
-LRB- |
denotes |
[ |
T5682 |
21395-21397 |
CD |
denotes |
39 |
T5683 |
21394-21395 |
-LRB- |
denotes |
[ |
T5684 |
21397-21398 |
-RRB- |
denotes |
] |
T5685 |
21398-21399 |
: |
denotes |
; |
T5686 |
21400-21403 |
CC |
denotes |
and |
T5687 |
21404-21408 |
NNS |
denotes |
data |
T5688 |
21409-21420 |
JJ |
denotes |
unpublished |
T5689 |
21420-21421 |
-RRB- |
denotes |
] |
T5690 |
21421-21422 |
. |
denotes |
. |
T5691 |
21422-21584 |
sentence |
denotes |
It might well be that the observed fusion of phospholipid layers by annexin A7 in the presence of high Ca2+ concentrations may turn out to be an in vitro effect. |
T5692 |
21423-21425 |
PRP |
denotes |
It |
T5693 |
21437-21439 |
VB |
denotes |
be |
T5694 |
21426-21431 |
MD |
denotes |
might |
T5695 |
21432-21436 |
RB |
denotes |
well |
T5696 |
21440-21444 |
IN |
denotes |
that |
T5697 |
21550-21554 |
VB |
denotes |
turn |
T5698 |
21445-21448 |
DT |
denotes |
the |
T5699 |
21458-21464 |
NN |
denotes |
fusion |
T5700 |
21449-21457 |
VBN |
denotes |
observed |
T5701 |
21465-21467 |
IN |
denotes |
of |
T5702 |
21468-21480 |
NN |
denotes |
phospholipid |
T5703 |
21481-21487 |
NNS |
denotes |
layers |
T5704 |
21488-21490 |
IN |
denotes |
by |
T5705 |
21491-21498 |
NN |
denotes |
annexin |
T5706 |
21499-21501 |
NN |
denotes |
A7 |
T5707 |
21502-21504 |
IN |
denotes |
in |
T5708 |
21505-21508 |
DT |
denotes |
the |
T5709 |
21509-21517 |
NN |
denotes |
presence |
T5710 |
21518-21520 |
IN |
denotes |
of |
T5711 |
21521-21525 |
JJ |
denotes |
high |
T5712 |
21531-21545 |
NNS |
denotes |
concentrations |
T5713 |
21526-21530 |
NN |
denotes |
Ca2+ |
T5714 |
21546-21549 |
MD |
denotes |
may |
T5715 |
21555-21558 |
RP |
denotes |
out |
T5716 |
21559-21561 |
TO |
denotes |
to |
T5717 |
21562-21564 |
VB |
denotes |
be |
T5718 |
21565-21567 |
DT |
denotes |
an |
T5719 |
21577-21583 |
NN |
denotes |
effect |
T5720 |
21568-21570 |
FW |
denotes |
in |
T5721 |
21571-21576 |
FW |
denotes |
vitro |
T5722 |
21583-21584 |
. |
denotes |
. |
T5723 |
21584-21851 |
sentence |
denotes |
Similarly, the high abundance of annexin A7 in micro- and nanovesicles might be fortuitous as a significant percentage of the red blood cell membrane, with which annexin A7 associates when the Ca2+ levels rise, appears to be involved in the formation of exovesicles. |
T5724 |
21585-21594 |
RB |
denotes |
Similarly |
T5725 |
21662-21664 |
VB |
denotes |
be |
T5726 |
21594-21596 |
, |
denotes |
, |
T5727 |
21596-21599 |
DT |
denotes |
the |
T5728 |
21605-21614 |
NN |
denotes |
abundance |
T5729 |
21600-21604 |
JJ |
denotes |
high |
T5730 |
21615-21617 |
IN |
denotes |
of |
T5731 |
21618-21625 |
NN |
denotes |
annexin |
T5732 |
21626-21628 |
NN |
denotes |
A7 |
T5733 |
21629-21631 |
IN |
denotes |
in |
T5734 |
21632-21637 |
AFX |
denotes |
micro |
T5735 |
21643-21655 |
NNS |
denotes |
nanovesicles |
T5736 |
21637-21638 |
HYPH |
denotes |
- |
T5737 |
21639-21642 |
CC |
denotes |
and |
T5738 |
21656-21661 |
MD |
denotes |
might |
T5739 |
21665-21675 |
JJ |
denotes |
fortuitous |
T5740 |
21676-21678 |
IN |
denotes |
as |
T5741 |
21796-21803 |
VBZ |
denotes |
appears |
T5742 |
21679-21680 |
DT |
denotes |
a |
T5743 |
21693-21703 |
NN |
denotes |
percentage |
T5744 |
21681-21692 |
JJ |
denotes |
significant |
T5745 |
21704-21706 |
IN |
denotes |
of |
T5746 |
21707-21710 |
DT |
denotes |
the |
T5747 |
21726-21734 |
NN |
denotes |
membrane |
T5748 |
21711-21714 |
JJ |
denotes |
red |
T5749 |
21721-21725 |
NN |
denotes |
cell |
T5750 |
21715-21720 |
NN |
denotes |
blood |
T5751 |
21734-21736 |
, |
denotes |
, |
T5752 |
21736-21740 |
IN |
denotes |
with |
T5753 |
21758-21768 |
VBZ |
denotes |
associates |
T5754 |
21741-21746 |
WDT |
denotes |
which |
T5755 |
21747-21754 |
NN |
denotes |
annexin |
T5756 |
21755-21757 |
NN |
denotes |
A7 |
T5757 |
21769-21773 |
WRB |
denotes |
when |
T5758 |
21790-21794 |
VBP |
denotes |
rise |
T5759 |
21774-21777 |
DT |
denotes |
the |
T5760 |
21783-21789 |
NNS |
denotes |
levels |
T5761 |
21778-21782 |
NN |
denotes |
Ca2+ |
T5762 |
21794-21796 |
, |
denotes |
, |
T5763 |
21804-21806 |
TO |
denotes |
to |
T5764 |
21810-21818 |
VBN |
denotes |
involved |
T5765 |
21807-21809 |
VB |
denotes |
be |
T5766 |
21819-21821 |
IN |
denotes |
in |
T5767 |
21822-21825 |
DT |
denotes |
the |
T5768 |
21826-21835 |
NN |
denotes |
formation |
T5769 |
21836-21838 |
IN |
denotes |
of |
T5770 |
21839-21850 |
NNS |
denotes |
exovesicles |
T5771 |
21850-21851 |
. |
denotes |
. |
T5772 |
21851-22036 |
sentence |
denotes |
Furthermore, the distribution of both annexin A7 isoforms in gradients of red blood cells indicates a function which differs from organizing subcellular membranes or membrane pathways. |
T5773 |
21852-21863 |
RB |
denotes |
Furthermore |
T5774 |
21942-21951 |
VBZ |
denotes |
indicates |
T5775 |
21863-21865 |
, |
denotes |
, |
T5776 |
21865-21868 |
DT |
denotes |
the |
T5777 |
21869-21881 |
NN |
denotes |
distribution |
T5778 |
21882-21884 |
IN |
denotes |
of |
T5779 |
21885-21889 |
DT |
denotes |
both |
T5780 |
21901-21909 |
NNS |
denotes |
isoforms |
T5781 |
21890-21897 |
NN |
denotes |
annexin |
T5782 |
21898-21900 |
NN |
denotes |
A7 |
T5783 |
21910-21912 |
IN |
denotes |
in |
T5784 |
21913-21922 |
NNS |
denotes |
gradients |
T5785 |
21923-21925 |
IN |
denotes |
of |
T5786 |
21926-21929 |
JJ |
denotes |
red |
T5787 |
21936-21941 |
NNS |
denotes |
cells |
T5788 |
21930-21935 |
NN |
denotes |
blood |
T5789 |
21952-21953 |
DT |
denotes |
a |
T5790 |
21954-21962 |
NN |
denotes |
function |
T5791 |
21963-21968 |
WDT |
denotes |
which |
T5792 |
21969-21976 |
VBZ |
denotes |
differs |
T5793 |
21977-21981 |
IN |
denotes |
from |
T5794 |
21982-21992 |
VBG |
denotes |
organizing |
T5795 |
21993-22004 |
JJ |
denotes |
subcellular |
T5796 |
22005-22014 |
NNS |
denotes |
membranes |
T5797 |
22015-22017 |
CC |
denotes |
or |
T5798 |
22018-22026 |
NN |
denotes |
membrane |
T5799 |
22027-22035 |
NNS |
denotes |
pathways |
T5800 |
22035-22036 |
. |
denotes |
. |
T5801 |
22036-22252 |
sentence |
denotes |
Whereas annexin A2 is tightly associated with endosomes and may have functions in membrane fusion and the endosomal pathway, annexin A7 distributes with plasma membrane raft domains of different types and densities. |
T5802 |
22037-22044 |
IN |
denotes |
Whereas |
T5803 |
22067-22077 |
VBN |
denotes |
associated |
T5804 |
22045-22052 |
NN |
denotes |
annexin |
T5805 |
22053-22055 |
NN |
denotes |
A2 |
T5806 |
22056-22058 |
VBZ |
denotes |
is |
T5807 |
22059-22066 |
RB |
denotes |
tightly |
T5808 |
22173-22184 |
VBZ |
denotes |
distributes |
T5809 |
22078-22082 |
IN |
denotes |
with |
T5810 |
22083-22092 |
NNS |
denotes |
endosomes |
T5811 |
22093-22096 |
CC |
denotes |
and |
T5812 |
22097-22100 |
MD |
denotes |
may |
T5813 |
22101-22105 |
VB |
denotes |
have |
T5814 |
22106-22115 |
NNS |
denotes |
functions |
T5815 |
22116-22118 |
IN |
denotes |
in |
T5816 |
22119-22127 |
NN |
denotes |
membrane |
T5817 |
22128-22134 |
NN |
denotes |
fusion |
T5818 |
22135-22138 |
CC |
denotes |
and |
T5819 |
22139-22142 |
DT |
denotes |
the |
T5820 |
22153-22160 |
NN |
denotes |
pathway |
T5821 |
22143-22152 |
JJ |
denotes |
endosomal |
T5822 |
22160-22162 |
, |
denotes |
, |
T5823 |
22162-22169 |
NN |
denotes |
annexin |
T5824 |
22170-22172 |
NN |
denotes |
A7 |
T5825 |
22185-22189 |
IN |
denotes |
with |
T5826 |
22190-22196 |
NN |
denotes |
plasma |
T5827 |
22197-22205 |
NN |
denotes |
membrane |
T5828 |
22211-22218 |
NNS |
denotes |
domains |
T5829 |
22206-22210 |
NN |
denotes |
raft |
T5830 |
22219-22221 |
IN |
denotes |
of |
T5831 |
22222-22231 |
JJ |
denotes |
different |
T5832 |
22232-22237 |
NNS |
denotes |
types |
T5833 |
22238-22241 |
CC |
denotes |
and |
T5834 |
22242-22251 |
NNS |
denotes |
densities |
T5835 |
22251-22252 |
. |
denotes |
. |
T5836 |
22252-22486 |
sentence |
denotes |
Interestingly, in vesicles of red blood cells lacking annexin A7 the amount of sorcin is lowered, indicating that its localisation is affected by the loss of annexin A7 or its expression during red blood cell development was reduced. |
T5837 |
22253-22266 |
RB |
denotes |
Interestingly |
T5838 |
22342-22349 |
VBN |
denotes |
lowered |
T5839 |
22266-22268 |
, |
denotes |
, |
T5840 |
22268-22270 |
IN |
denotes |
in |
T5841 |
22271-22279 |
NNS |
denotes |
vesicles |
T5842 |
22280-22282 |
IN |
denotes |
of |
T5843 |
22283-22286 |
JJ |
denotes |
red |
T5844 |
22293-22298 |
NNS |
denotes |
cells |
T5845 |
22287-22292 |
NN |
denotes |
blood |
T5846 |
22299-22306 |
VBG |
denotes |
lacking |
T5847 |
22307-22314 |
NN |
denotes |
annexin |
T5848 |
22315-22317 |
NN |
denotes |
A7 |
T5849 |
22318-22321 |
DT |
denotes |
the |
T5850 |
22322-22328 |
NN |
denotes |
amount |
T5851 |
22329-22331 |
IN |
denotes |
of |
T5852 |
22332-22338 |
NN |
denotes |
sorcin |
T5853 |
22339-22341 |
VBZ |
denotes |
is |
T5854 |
22349-22351 |
, |
denotes |
, |
T5855 |
22351-22361 |
VBG |
denotes |
indicating |
T5856 |
22362-22366 |
IN |
denotes |
that |
T5857 |
22387-22395 |
VBN |
denotes |
affected |
T5858 |
22367-22370 |
PRP$ |
denotes |
its |
T5859 |
22371-22383 |
NN |
denotes |
localisation |
T5860 |
22384-22386 |
VBZ |
denotes |
is |
T5861 |
22396-22398 |
IN |
denotes |
by |
T5862 |
22399-22402 |
DT |
denotes |
the |
T5863 |
22403-22407 |
NN |
denotes |
loss |
T5864 |
22408-22410 |
IN |
denotes |
of |
T5865 |
22411-22418 |
NN |
denotes |
annexin |
T5866 |
22419-22421 |
NN |
denotes |
A7 |
T5867 |
22422-22424 |
CC |
denotes |
or |
T5868 |
22425-22428 |
PRP$ |
denotes |
its |
T5869 |
22429-22439 |
NN |
denotes |
expression |
T5870 |
22478-22485 |
VBN |
denotes |
reduced |
T5871 |
22440-22446 |
IN |
denotes |
during |
T5872 |
22447-22450 |
JJ |
denotes |
red |
T5873 |
22457-22461 |
NN |
denotes |
cell |
T5874 |
22451-22456 |
NN |
denotes |
blood |
T5875 |
22462-22473 |
NN |
denotes |
development |
T5876 |
22474-22477 |
VBD |
denotes |
was |
T5877 |
22485-22486 |
. |
denotes |
. |
T5878 |
22486-22625 |
sentence |
denotes |
Having excluded a direct role in vesicle formation and membrane fusion, annexin A7 might act by its property as Ca2+-binding protein [17]. |
T5879 |
22487-22493 |
VBG |
denotes |
Having |
T5880 |
22494-22502 |
VBN |
denotes |
excluded |
T5881 |
22576-22579 |
VB |
denotes |
act |
T5882 |
22503-22504 |
DT |
denotes |
a |
T5883 |
22512-22516 |
NN |
denotes |
role |
T5884 |
22505-22511 |
JJ |
denotes |
direct |
T5885 |
22517-22519 |
IN |
denotes |
in |
T5886 |
22520-22527 |
NN |
denotes |
vesicle |
T5887 |
22528-22537 |
NN |
denotes |
formation |
T5888 |
22538-22541 |
CC |
denotes |
and |
T5889 |
22542-22550 |
NN |
denotes |
membrane |
T5890 |
22551-22557 |
NN |
denotes |
fusion |
T5891 |
22557-22559 |
, |
denotes |
, |
T5892 |
22559-22566 |
NN |
denotes |
annexin |
T5893 |
22567-22569 |
NN |
denotes |
A7 |
T5894 |
22570-22575 |
MD |
denotes |
might |
T5895 |
22580-22582 |
IN |
denotes |
by |
T5896 |
22583-22586 |
PRP$ |
denotes |
its |
T5897 |
22587-22595 |
NN |
denotes |
property |
T5898 |
22596-22598 |
IN |
denotes |
as |
T5899 |
22599-22603 |
NN |
denotes |
Ca2+ |
T5900 |
22604-22611 |
VBG |
denotes |
binding |
T5901 |
22603-22604 |
HYPH |
denotes |
- |
T5902 |
22612-22619 |
NN |
denotes |
protein |
T5903 |
22620-22621 |
-LRB- |
denotes |
[ |
T5904 |
22621-22623 |
CD |
denotes |
17 |
T5905 |
22623-22624 |
-RRB- |
denotes |
] |
T5906 |
22624-22625 |
. |
denotes |
. |
T5907 |
22625-22863 |
sentence |
denotes |
Previous results in which annexin A7 was shown to play a role in membrane aggregation might be explained by a supportive function of annexin A7 in that it interferes with the local Ca2+ homeostasis thus influencing membrane organization. |
T5908 |
22626-22634 |
JJ |
denotes |
Previous |
T5909 |
22635-22642 |
NNS |
denotes |
results |
T5910 |
22721-22730 |
VBN |
denotes |
explained |
T5911 |
22643-22645 |
IN |
denotes |
in |
T5912 |
22667-22672 |
VBN |
denotes |
shown |
T5913 |
22646-22651 |
WDT |
denotes |
which |
T5914 |
22652-22659 |
NN |
denotes |
annexin |
T5915 |
22660-22662 |
NN |
denotes |
A7 |
T5916 |
22663-22666 |
VBD |
denotes |
was |
T5917 |
22673-22675 |
TO |
denotes |
to |
T5918 |
22676-22680 |
VB |
denotes |
play |
T5919 |
22681-22682 |
DT |
denotes |
a |
T5920 |
22683-22687 |
NN |
denotes |
role |
T5921 |
22688-22690 |
IN |
denotes |
in |
T5922 |
22691-22699 |
NN |
denotes |
membrane |
T5923 |
22700-22711 |
NN |
denotes |
aggregation |
T5924 |
22712-22717 |
MD |
denotes |
might |
T5925 |
22718-22720 |
VB |
denotes |
be |
T5926 |
22731-22733 |
IN |
denotes |
by |
T5927 |
22734-22735 |
DT |
denotes |
a |
T5928 |
22747-22755 |
NN |
denotes |
function |
T5929 |
22736-22746 |
JJ |
denotes |
supportive |
T5930 |
22756-22758 |
IN |
denotes |
of |
T5931 |
22759-22766 |
NN |
denotes |
annexin |
T5932 |
22767-22769 |
NN |
denotes |
A7 |
T5933 |
22770-22772 |
IN |
denotes |
in |
T5934 |
22781-22791 |
VBZ |
denotes |
interferes |
T5935 |
22773-22777 |
IN |
denotes |
that |
T5936 |
22778-22780 |
PRP |
denotes |
it |
T5937 |
22792-22796 |
IN |
denotes |
with |
T5938 |
22797-22800 |
DT |
denotes |
the |
T5939 |
22812-22823 |
NN |
denotes |
homeostasis |
T5940 |
22801-22806 |
JJ |
denotes |
local |
T5941 |
22807-22811 |
NN |
denotes |
Ca2+ |
T5942 |
22824-22828 |
RB |
denotes |
thus |
T5943 |
22829-22840 |
VBG |
denotes |
influencing |
T5944 |
22841-22849 |
NN |
denotes |
membrane |
T5945 |
22850-22862 |
NN |
denotes |
organization |
T5946 |
22862-22863 |
. |
denotes |
. |
T5947 |
22863-23001 |
sentence |
denotes |
As the annexins belong to a wide spread and evolutionarily conserved protein family, redundant, but not identical functions are expected. |
T5948 |
22864-22866 |
IN |
denotes |
As |
T5949 |
22880-22886 |
VBP |
denotes |
belong |
T5950 |
22867-22870 |
DT |
denotes |
the |
T5951 |
22871-22879 |
NNS |
denotes |
annexins |
T5952 |
22992-23000 |
VBN |
denotes |
expected |
T5953 |
22887-22889 |
IN |
denotes |
to |
T5954 |
22890-22891 |
DT |
denotes |
a |
T5955 |
22941-22947 |
NN |
denotes |
family |
T5956 |
22892-22896 |
RB |
denotes |
wide |
T5957 |
22897-22903 |
VBN |
denotes |
spread |
T5958 |
22904-22907 |
CC |
denotes |
and |
T5959 |
22908-22922 |
RB |
denotes |
evolutionarily |
T5960 |
22923-22932 |
VBN |
denotes |
conserved |
T5961 |
22933-22940 |
NN |
denotes |
protein |
T5962 |
22947-22949 |
, |
denotes |
, |
T5963 |
22949-22958 |
NN |
denotes |
redundant |
T5964 |
22968-22977 |
JJ |
denotes |
identical |
T5965 |
22958-22960 |
, |
denotes |
, |
T5966 |
22960-22963 |
CC |
denotes |
but |
T5967 |
22964-22967 |
RB |
denotes |
not |
T5968 |
22978-22987 |
NNS |
denotes |
functions |
T5969 |
22988-22991 |
VBP |
denotes |
are |
T5970 |
23000-23001 |
. |
denotes |
. |
T5971 |
23001-23134 |
sentence |
denotes |
However, so far no other members of the annexin family, which may substitute for annexin A7, have been described in red blood cells. |
T5972 |
23002-23009 |
RB |
denotes |
However |
T5973 |
23105-23114 |
VBN |
denotes |
described |
T5974 |
23009-23011 |
, |
denotes |
, |
T5975 |
23011-23013 |
RB |
denotes |
so |
T5976 |
23014-23017 |
RB |
denotes |
far |
T5977 |
23018-23020 |
DT |
denotes |
no |
T5978 |
23027-23034 |
NNS |
denotes |
members |
T5979 |
23021-23026 |
JJ |
denotes |
other |
T5980 |
23035-23037 |
IN |
denotes |
of |
T5981 |
23038-23041 |
DT |
denotes |
the |
T5982 |
23050-23056 |
NN |
denotes |
family |
T5983 |
23042-23049 |
NN |
denotes |
annexin |
T5984 |
23056-23058 |
, |
denotes |
, |
T5985 |
23058-23063 |
WDT |
denotes |
which |
T5986 |
23068-23078 |
VB |
denotes |
substitute |
T5987 |
23064-23067 |
MD |
denotes |
may |
T5988 |
23079-23082 |
IN |
denotes |
for |
T5989 |
23083-23090 |
NN |
denotes |
annexin |
T5990 |
23091-23093 |
NN |
denotes |
A7 |
T5991 |
23093-23095 |
, |
denotes |
, |
T5992 |
23095-23099 |
VBP |
denotes |
have |
T5993 |
23100-23104 |
VBN |
denotes |
been |
T5994 |
23115-23117 |
IN |
denotes |
in |
T5995 |
23118-23121 |
JJ |
denotes |
red |
T5996 |
23128-23133 |
NNS |
denotes |
cells |
T5997 |
23122-23127 |
NN |
denotes |
blood |
T5998 |
23133-23134 |
. |
denotes |
. |
T5999 |
23134-23326 |
sentence |
denotes |
The structural basis for the elasticy of red blood cells in circulation are long range molecular functions of the plasma membrane and the membrane associated part of the cytoskeleton [40-43]. |
T6000 |
23135-23138 |
DT |
denotes |
The |
T6001 |
23150-23155 |
NN |
denotes |
basis |
T6002 |
23139-23149 |
JJ |
denotes |
structural |
T6003 |
23207-23210 |
VBP |
denotes |
are |
T6004 |
23156-23159 |
IN |
denotes |
for |
T6005 |
23160-23163 |
DT |
denotes |
the |
T6006 |
23164-23172 |
NN |
denotes |
elasticy |
T6007 |
23173-23175 |
IN |
denotes |
of |
T6008 |
23176-23179 |
JJ |
denotes |
red |
T6009 |
23186-23191 |
NNS |
denotes |
cells |
T6010 |
23180-23185 |
NN |
denotes |
blood |
T6011 |
23192-23194 |
IN |
denotes |
in |
T6012 |
23195-23206 |
NN |
denotes |
circulation |
T6013 |
23211-23215 |
JJ |
denotes |
long |
T6014 |
23216-23221 |
NN |
denotes |
range |
T6015 |
23232-23241 |
NNS |
denotes |
functions |
T6016 |
23222-23231 |
JJ |
denotes |
molecular |
T6017 |
23242-23244 |
IN |
denotes |
of |
T6018 |
23245-23248 |
DT |
denotes |
the |
T6019 |
23256-23264 |
NN |
denotes |
membrane |
T6020 |
23249-23255 |
NN |
denotes |
plasma |
T6021 |
23265-23268 |
CC |
denotes |
and |
T6022 |
23269-23272 |
DT |
denotes |
the |
T6023 |
23293-23297 |
NN |
denotes |
part |
T6024 |
23273-23281 |
NN |
denotes |
membrane |
T6025 |
23282-23292 |
VBN |
denotes |
associated |
T6026 |
23298-23300 |
IN |
denotes |
of |
T6027 |
23301-23304 |
DT |
denotes |
the |
T6028 |
23305-23317 |
NN |
denotes |
cytoskeleton |
T6029 |
23318-23319 |
-LRB- |
denotes |
[ |
T6030 |
23319-23321 |
CD |
denotes |
40 |
T6031 |
23321-23322 |
SYM |
denotes |
- |
T6032 |
23322-23324 |
CD |
denotes |
43 |
T6033 |
23324-23325 |
-RRB- |
denotes |
] |
T6034 |
23325-23326 |
. |
denotes |
. |
T6035 |
23326-23538 |
sentence |
denotes |
Major constituents of the membrane skeleton are spectrin tetramers linked together by short actin filaments and several other proteins covering the entire cytoplasmic surface of the red blood cell membrane [44]. |
T6036 |
23327-23332 |
JJ |
denotes |
Major |
T6037 |
23333-23345 |
NNS |
denotes |
constituents |
T6038 |
23371-23374 |
VBP |
denotes |
are |
T6039 |
23346-23348 |
IN |
denotes |
of |
T6040 |
23349-23352 |
DT |
denotes |
the |
T6041 |
23362-23370 |
NN |
denotes |
skeleton |
T6042 |
23353-23361 |
NN |
denotes |
membrane |
T6043 |
23375-23383 |
NN |
denotes |
spectrin |
T6044 |
23384-23393 |
NNS |
denotes |
tetramers |
T6045 |
23394-23400 |
VBN |
denotes |
linked |
T6046 |
23401-23409 |
RB |
denotes |
together |
T6047 |
23410-23412 |
IN |
denotes |
by |
T6048 |
23413-23418 |
JJ |
denotes |
short |
T6049 |
23425-23434 |
NNS |
denotes |
filaments |
T6050 |
23419-23424 |
NN |
denotes |
actin |
T6051 |
23435-23438 |
CC |
denotes |
and |
T6052 |
23439-23446 |
JJ |
denotes |
several |
T6053 |
23453-23461 |
NN |
denotes |
proteins |
T6054 |
23447-23452 |
JJ |
denotes |
other |
T6055 |
23462-23470 |
VBG |
denotes |
covering |
T6056 |
23471-23474 |
DT |
denotes |
the |
T6057 |
23494-23501 |
NN |
denotes |
surface |
T6058 |
23475-23481 |
JJ |
denotes |
entire |
T6059 |
23482-23493 |
JJ |
denotes |
cytoplasmic |
T6060 |
23502-23504 |
IN |
denotes |
of |
T6061 |
23505-23508 |
DT |
denotes |
the |
T6062 |
23524-23532 |
NN |
denotes |
membrane |
T6063 |
23509-23512 |
JJ |
denotes |
red |
T6064 |
23519-23523 |
NN |
denotes |
cell |
T6065 |
23513-23518 |
NN |
denotes |
blood |
T6066 |
23533-23534 |
-LRB- |
denotes |
[ |
T6067 |
23534-23536 |
CD |
denotes |
44 |
T6068 |
23536-23537 |
-RRB- |
denotes |
] |
T6069 |
23537-23538 |
. |
denotes |
. |
T6070 |
23538-23800 |
sentence |
denotes |
Membrane cholesterol diminishes red blood cell haemolysis by the complement complex, whereas depletion of raft cholesterol abrogates association of all raft proteins with no significant effect on areas in the rest of the cell membrane and deformability [45,46]. |
T6071 |
23539-23547 |
NN |
denotes |
Membrane |
T6072 |
23548-23559 |
NN |
denotes |
cholesterol |
T6073 |
23560-23570 |
VBZ |
denotes |
diminishes |
T6074 |
23571-23574 |
JJ |
denotes |
red |
T6075 |
23581-23585 |
NN |
denotes |
cell |
T6076 |
23575-23580 |
NN |
denotes |
blood |
T6077 |
23586-23596 |
NN |
denotes |
haemolysis |
T6078 |
23597-23599 |
IN |
denotes |
by |
T6079 |
23600-23603 |
DT |
denotes |
the |
T6080 |
23615-23622 |
NN |
denotes |
complex |
T6081 |
23604-23614 |
NN |
denotes |
complement |
T6082 |
23622-23624 |
, |
denotes |
, |
T6083 |
23624-23631 |
IN |
denotes |
whereas |
T6084 |
23662-23671 |
VBZ |
denotes |
abrogates |
T6085 |
23632-23641 |
NN |
denotes |
depletion |
T6086 |
23642-23644 |
IN |
denotes |
of |
T6087 |
23645-23649 |
NN |
denotes |
raft |
T6088 |
23650-23661 |
NN |
denotes |
cholesterol |
T6089 |
23672-23683 |
NN |
denotes |
association |
T6090 |
23684-23686 |
IN |
denotes |
of |
T6091 |
23687-23690 |
DT |
denotes |
all |
T6092 |
23696-23704 |
NN |
denotes |
proteins |
T6093 |
23691-23695 |
NN |
denotes |
raft |
T6094 |
23705-23709 |
IN |
denotes |
with |
T6095 |
23710-23712 |
DT |
denotes |
no |
T6096 |
23725-23731 |
NN |
denotes |
effect |
T6097 |
23713-23724 |
JJ |
denotes |
significant |
T6098 |
23732-23734 |
IN |
denotes |
on |
T6099 |
23735-23740 |
NNS |
denotes |
areas |
T6100 |
23741-23743 |
IN |
denotes |
in |
T6101 |
23744-23747 |
DT |
denotes |
the |
T6102 |
23748-23752 |
NN |
denotes |
rest |
T6103 |
23753-23755 |
IN |
denotes |
of |
T6104 |
23756-23759 |
DT |
denotes |
the |
T6105 |
23765-23773 |
NN |
denotes |
membrane |
T6106 |
23760-23764 |
NN |
denotes |
cell |
T6107 |
23774-23777 |
CC |
denotes |
and |
T6108 |
23778-23791 |
NN |
denotes |
deformability |
T6109 |
23792-23793 |
-LRB- |
denotes |
[ |
T6110 |
23796-23798 |
CD |
denotes |
46 |
T6111 |
23793-23795 |
CD |
denotes |
45 |
T6112 |
23795-23796 |
, |
denotes |
, |
T6113 |
23798-23799 |
-RRB- |
denotes |
] |
T6114 |
23799-23800 |
. |
denotes |
. |
T6115 |
23800-23904 |
sentence |
denotes |
Annexin A7 knock out mouse red blood cells show a more flat shape and have a higher osmotic resistance. |
T6116 |
23801-23808 |
NN |
denotes |
Annexin |
T6117 |
23809-23811 |
NN |
denotes |
A7 |
T6118 |
23838-23843 |
NNS |
denotes |
cells |
T6119 |
23812-23817 |
NN |
denotes |
knock |
T6120 |
23818-23821 |
NN |
denotes |
out |
T6121 |
23822-23827 |
NN |
denotes |
mouse |
T6122 |
23828-23831 |
JJ |
denotes |
red |
T6123 |
23832-23837 |
NN |
denotes |
blood |
T6124 |
23844-23848 |
VBP |
denotes |
show |
T6125 |
23849-23850 |
DT |
denotes |
a |
T6126 |
23861-23866 |
NN |
denotes |
shape |
T6127 |
23851-23855 |
RBR |
denotes |
more |
T6128 |
23856-23860 |
JJ |
denotes |
flat |
T6129 |
23867-23870 |
CC |
denotes |
and |
T6130 |
23871-23875 |
VBP |
denotes |
have |
T6131 |
23876-23877 |
DT |
denotes |
a |
T6132 |
23893-23903 |
NN |
denotes |
resistance |
T6133 |
23878-23884 |
JJR |
denotes |
higher |
T6134 |
23885-23892 |
JJ |
denotes |
osmotic |
T6135 |
23903-23904 |
. |
denotes |
. |
T6136 |
23904-24072 |
sentence |
denotes |
The presence of annexin A7 may alter the membrane flexibility in that it supports the typical biconcave membrane shape and leads to a lower minimal osmotic resistance. |
T6137 |
23905-23908 |
DT |
denotes |
The |
T6138 |
23909-23917 |
NN |
denotes |
presence |
T6139 |
23936-23941 |
VB |
denotes |
alter |
T6140 |
23918-23920 |
IN |
denotes |
of |
T6141 |
23921-23928 |
NN |
denotes |
annexin |
T6142 |
23929-23931 |
NN |
denotes |
A7 |
T6143 |
23932-23935 |
MD |
denotes |
may |
T6144 |
23942-23945 |
DT |
denotes |
the |
T6145 |
23955-23966 |
NN |
denotes |
flexibility |
T6146 |
23946-23954 |
NN |
denotes |
membrane |
T6147 |
23967-23969 |
IN |
denotes |
in |
T6148 |
23978-23986 |
VBZ |
denotes |
supports |
T6149 |
23970-23974 |
IN |
denotes |
that |
T6150 |
23975-23977 |
PRP |
denotes |
it |
T6151 |
23987-23990 |
DT |
denotes |
the |
T6152 |
24018-24023 |
NN |
denotes |
shape |
T6153 |
23991-23998 |
JJ |
denotes |
typical |
T6154 |
23999-24008 |
NN |
denotes |
biconcave |
T6155 |
24009-24017 |
NN |
denotes |
membrane |
T6156 |
24024-24027 |
CC |
denotes |
and |
T6157 |
24028-24033 |
VBZ |
denotes |
leads |
T6158 |
24034-24036 |
IN |
denotes |
to |
T6159 |
24037-24038 |
DT |
denotes |
a |
T6160 |
24061-24071 |
NN |
denotes |
resistance |
T6161 |
24039-24044 |
JJR |
denotes |
lower |
T6162 |
24045-24052 |
JJ |
denotes |
minimal |
T6163 |
24053-24060 |
JJ |
denotes |
osmotic |
T6164 |
24071-24072 |
. |
denotes |
. |
T6165 |
24072-24212 |
sentence |
denotes |
Annexin A7 does not appear to have an influence on plasma membrane integrity itself as the maximal osmotic resistance value stays constant. |
T6166 |
24073-24080 |
NN |
denotes |
Annexin |
T6167 |
24081-24083 |
NN |
denotes |
A7 |
T6168 |
24093-24099 |
VB |
denotes |
appear |
T6169 |
24084-24088 |
VBZ |
denotes |
does |
T6170 |
24089-24092 |
RB |
denotes |
not |
T6171 |
24100-24102 |
TO |
denotes |
to |
T6172 |
24103-24107 |
VB |
denotes |
have |
T6173 |
24108-24110 |
DT |
denotes |
an |
T6174 |
24111-24120 |
NN |
denotes |
influence |
T6175 |
24121-24123 |
IN |
denotes |
on |
T6176 |
24124-24130 |
NN |
denotes |
plasma |
T6177 |
24131-24139 |
NN |
denotes |
membrane |
T6178 |
24140-24149 |
NN |
denotes |
integrity |
T6179 |
24150-24156 |
PRP |
denotes |
itself |
T6180 |
24157-24159 |
IN |
denotes |
as |
T6181 |
24197-24202 |
VBZ |
denotes |
stays |
T6182 |
24160-24163 |
DT |
denotes |
the |
T6183 |
24191-24196 |
NN |
denotes |
value |
T6184 |
24164-24171 |
JJ |
denotes |
maximal |
T6185 |
24172-24179 |
JJ |
denotes |
osmotic |
T6186 |
24180-24190 |
NN |
denotes |
resistance |
T6187 |
24203-24211 |
JJ |
denotes |
constant |
T6188 |
24211-24212 |
. |
denotes |
. |
T6189 |
24212-24333 |
sentence |
denotes |
Ca2+ and membrane skeletal proteins are known to play key roles on the red blood cell membrane shape and stability [47]. |
T6190 |
24213-24217 |
NN |
denotes |
Ca2+ |
T6191 |
24240-24248 |
NN |
denotes |
proteins |
T6192 |
24218-24221 |
CC |
denotes |
and |
T6193 |
24222-24230 |
NN |
denotes |
membrane |
T6194 |
24231-24239 |
JJ |
denotes |
skeletal |
T6195 |
24253-24258 |
VBN |
denotes |
known |
T6196 |
24249-24252 |
VBP |
denotes |
are |
T6197 |
24259-24261 |
TO |
denotes |
to |
T6198 |
24262-24266 |
VB |
denotes |
play |
T6199 |
24267-24270 |
JJ |
denotes |
key |
T6200 |
24271-24276 |
NNS |
denotes |
roles |
T6201 |
24277-24279 |
IN |
denotes |
on |
T6202 |
24280-24283 |
DT |
denotes |
the |
T6203 |
24308-24313 |
NN |
denotes |
shape |
T6204 |
24284-24287 |
JJ |
denotes |
red |
T6205 |
24294-24298 |
NN |
denotes |
cell |
T6206 |
24288-24293 |
NN |
denotes |
blood |
T6207 |
24299-24307 |
NN |
denotes |
membrane |
T6208 |
24314-24317 |
CC |
denotes |
and |
T6209 |
24318-24327 |
NN |
denotes |
stability |
T6210 |
24328-24329 |
-LRB- |
denotes |
[ |
T6211 |
24329-24331 |
CD |
denotes |
47 |
T6212 |
24331-24332 |
-RRB- |
denotes |
] |
T6213 |
24332-24333 |
. |
denotes |
. |
T6214 |
24333-24448 |
sentence |
denotes |
Annexins have been reported to bind directly to F-actin, we could however exclude such an activity for annexin A7. |
T6215 |
24334-24342 |
NNS |
denotes |
Annexins |
T6216 |
24353-24361 |
VBN |
denotes |
reported |
T6217 |
24343-24347 |
VBP |
denotes |
have |
T6218 |
24348-24352 |
VBN |
denotes |
been |
T6219 |
24408-24415 |
VB |
denotes |
exclude |
T6220 |
24362-24364 |
TO |
denotes |
to |
T6221 |
24365-24369 |
VB |
denotes |
bind |
T6222 |
24370-24378 |
RB |
denotes |
directly |
T6223 |
24379-24381 |
IN |
denotes |
to |
T6224 |
24382-24383 |
NN |
denotes |
F |
T6225 |
24384-24389 |
NN |
denotes |
actin |
T6226 |
24383-24384 |
HYPH |
denotes |
- |
T6227 |
24389-24391 |
, |
denotes |
, |
T6228 |
24391-24393 |
PRP |
denotes |
we |
T6229 |
24394-24399 |
MD |
denotes |
could |
T6230 |
24400-24407 |
RB |
denotes |
however |
T6231 |
24416-24420 |
JJ |
denotes |
such |
T6232 |
24424-24432 |
NN |
denotes |
activity |
T6233 |
24421-24423 |
DT |
denotes |
an |
T6234 |
24433-24436 |
IN |
denotes |
for |
T6235 |
24437-24444 |
NN |
denotes |
annexin |
T6236 |
24445-24447 |
NN |
denotes |
A7 |
T6237 |
24447-24448 |
. |
denotes |
. |
T6238 |
24448-24709 |
sentence |
denotes |
Nevertheless it might interact with other components and have a role in organizing the membrane cytoskeleton, controlling raft protein associations or influencing the ionic strength of red blood cells on its own or by interfering with other signaling pathways. |
T6239 |
24449-24461 |
RB |
denotes |
Nevertheless |
T6240 |
24471-24479 |
VB |
denotes |
interact |
T6241 |
24462-24464 |
PRP |
denotes |
it |
T6242 |
24465-24470 |
MD |
denotes |
might |
T6243 |
24480-24484 |
IN |
denotes |
with |
T6244 |
24485-24490 |
JJ |
denotes |
other |
T6245 |
24491-24501 |
NNS |
denotes |
components |
T6246 |
24502-24505 |
CC |
denotes |
and |
T6247 |
24506-24510 |
VBP |
denotes |
have |
T6248 |
24511-24512 |
DT |
denotes |
a |
T6249 |
24513-24517 |
NN |
denotes |
role |
T6250 |
24518-24520 |
IN |
denotes |
in |
T6251 |
24521-24531 |
VBG |
denotes |
organizing |
T6252 |
24532-24535 |
DT |
denotes |
the |
T6253 |
24545-24557 |
NN |
denotes |
cytoskeleton |
T6254 |
24536-24544 |
NN |
denotes |
membrane |
T6255 |
24557-24559 |
, |
denotes |
, |
T6256 |
24559-24570 |
VBG |
denotes |
controlling |
T6257 |
24571-24575 |
NN |
denotes |
raft |
T6258 |
24576-24583 |
NN |
denotes |
protein |
T6259 |
24584-24596 |
NNS |
denotes |
associations |
T6260 |
24597-24599 |
CC |
denotes |
or |
T6261 |
24600-24611 |
VBG |
denotes |
influencing |
T6262 |
24612-24615 |
DT |
denotes |
the |
T6263 |
24622-24630 |
NN |
denotes |
strength |
T6264 |
24616-24621 |
JJ |
denotes |
ionic |
T6265 |
24631-24633 |
IN |
denotes |
of |
T6266 |
24634-24637 |
JJ |
denotes |
red |
T6267 |
24644-24649 |
NNS |
denotes |
cells |
T6268 |
24638-24643 |
NN |
denotes |
blood |
T6269 |
24650-24652 |
IN |
denotes |
on |
T6270 |
24653-24656 |
PRP$ |
denotes |
its |
T6271 |
24657-24660 |
JJ |
denotes |
own |
T6272 |
24661-24663 |
CC |
denotes |
or |
T6273 |
24664-24666 |
IN |
denotes |
by |
T6274 |
24667-24678 |
VBG |
denotes |
interfering |
T6275 |
24679-24683 |
IN |
denotes |
with |
T6276 |
24684-24689 |
JJ |
denotes |
other |
T6277 |
24700-24708 |
NNS |
denotes |
pathways |
T6278 |
24690-24699 |
NN |
denotes |
signaling |
T6279 |
24708-24709 |
. |
denotes |
. |
T6280 |
24709-24794 |
sentence |
denotes |
As discussed above annexin A7 could be involved in the cytoplasmic Ca2+ homeostasis. |
T6281 |
24710-24712 |
IN |
denotes |
As |
T6282 |
24713-24722 |
VBN |
denotes |
discussed |
T6283 |
24749-24757 |
VBN |
denotes |
involved |
T6284 |
24723-24728 |
RB |
denotes |
above |
T6285 |
24729-24736 |
NN |
denotes |
annexin |
T6286 |
24737-24739 |
NN |
denotes |
A7 |
T6287 |
24740-24745 |
MD |
denotes |
could |
T6288 |
24746-24748 |
VB |
denotes |
be |
T6289 |
24758-24760 |
IN |
denotes |
in |
T6290 |
24761-24764 |
DT |
denotes |
the |
T6291 |
24782-24793 |
NN |
denotes |
homeostasis |
T6292 |
24765-24776 |
JJ |
denotes |
cytoplasmic |
T6293 |
24777-24781 |
NN |
denotes |
Ca2+ |
T6294 |
24793-24794 |
. |
denotes |
. |
T6295 |
24794-24986 |
sentence |
denotes |
It has been demonstrated that micromolar changes of the intracellular Ca2+ concentration exert a profound effect on the membrane properties that regulate red blood cell deformability [48,49]. |
T6296 |
24795-24797 |
PRP |
denotes |
It |
T6297 |
24807-24819 |
VBN |
denotes |
demonstrated |
T6298 |
24798-24801 |
VBZ |
denotes |
has |
T6299 |
24802-24806 |
VBN |
denotes |
been |
T6300 |
24820-24824 |
IN |
denotes |
that |
T6301 |
24884-24889 |
VBP |
denotes |
exert |
T6302 |
24825-24835 |
JJ |
denotes |
micromolar |
T6303 |
24836-24843 |
NNS |
denotes |
changes |
T6304 |
24844-24846 |
IN |
denotes |
of |
T6305 |
24847-24850 |
DT |
denotes |
the |
T6306 |
24870-24883 |
NN |
denotes |
concentration |
T6307 |
24851-24864 |
JJ |
denotes |
intracellular |
T6308 |
24865-24869 |
NN |
denotes |
Ca2+ |
T6309 |
24890-24891 |
DT |
denotes |
a |
T6310 |
24901-24907 |
NN |
denotes |
effect |
T6311 |
24892-24900 |
JJ |
denotes |
profound |
T6312 |
24908-24910 |
IN |
denotes |
on |
T6313 |
24911-24914 |
DT |
denotes |
the |
T6314 |
24924-24934 |
NNS |
denotes |
properties |
T6315 |
24915-24923 |
NN |
denotes |
membrane |
T6316 |
24935-24939 |
WDT |
denotes |
that |
T6317 |
24940-24948 |
VBP |
denotes |
regulate |
T6318 |
24949-24952 |
JJ |
denotes |
red |
T6319 |
24959-24963 |
NN |
denotes |
cell |
T6320 |
24953-24958 |
NN |
denotes |
blood |
T6321 |
24964-24977 |
NN |
denotes |
deformability |
T6322 |
24978-24979 |
-LRB- |
denotes |
[ |
T6323 |
24982-24984 |
CD |
denotes |
49 |
T6324 |
24979-24981 |
CD |
denotes |
48 |
T6325 |
24981-24982 |
, |
denotes |
, |
T6326 |
24984-24985 |
-RRB- |
denotes |
] |
T6327 |
24985-24986 |
. |
denotes |
. |
T6328 |
24986-25129 |
sentence |
denotes |
Furthermore, the intracellular Ca2+ was shown to regulate the membrane stability through modulation of cytoskeletal protein interactions [50]. |
T6329 |
24987-24998 |
RB |
denotes |
Furthermore |
T6330 |
25027-25032 |
VBN |
denotes |
shown |
T6331 |
24998-25000 |
, |
denotes |
, |
T6332 |
25000-25003 |
DT |
denotes |
the |
T6333 |
25018-25022 |
NN |
denotes |
Ca2+ |
T6334 |
25004-25017 |
JJ |
denotes |
intracellular |
T6335 |
25023-25026 |
VBD |
denotes |
was |
T6336 |
25033-25035 |
TO |
denotes |
to |
T6337 |
25036-25044 |
VB |
denotes |
regulate |
T6338 |
25045-25048 |
DT |
denotes |
the |
T6339 |
25058-25067 |
NN |
denotes |
stability |
T6340 |
25049-25057 |
NN |
denotes |
membrane |
T6341 |
25068-25075 |
IN |
denotes |
through |
T6342 |
25076-25086 |
NN |
denotes |
modulation |
T6343 |
25087-25089 |
IN |
denotes |
of |
T6344 |
25090-25102 |
JJ |
denotes |
cytoskeletal |
T6345 |
25103-25110 |
NN |
denotes |
protein |
T6346 |
25111-25123 |
NNS |
denotes |
interactions |
T6347 |
25124-25125 |
-LRB- |
denotes |
[ |
T6348 |
25125-25127 |
CD |
denotes |
50 |
T6349 |
25127-25128 |
-RRB- |
denotes |
] |
T6350 |
25128-25129 |
. |
denotes |
. |
T6351 |
25129-25239 |
sentence |
denotes |
Ca2+ also induces a transbilayer phospholipid redistribution inducing a shape change of red blood cells [51]. |
T6352 |
25130-25134 |
NN |
denotes |
Ca2+ |
T6353 |
25140-25147 |
VBZ |
denotes |
induces |
T6354 |
25135-25139 |
RB |
denotes |
also |
T6355 |
25148-25149 |
DT |
denotes |
a |
T6356 |
25176-25190 |
NN |
denotes |
redistribution |
T6357 |
25150-25162 |
NN |
denotes |
transbilayer |
T6358 |
25163-25175 |
NN |
denotes |
phospholipid |
T6359 |
25191-25199 |
VBG |
denotes |
inducing |
T6360 |
25200-25201 |
DT |
denotes |
a |
T6361 |
25208-25214 |
NN |
denotes |
change |
T6362 |
25202-25207 |
NN |
denotes |
shape |
T6363 |
25215-25217 |
IN |
denotes |
of |
T6364 |
25218-25221 |
JJ |
denotes |
red |
T6365 |
25228-25233 |
NNS |
denotes |
cells |
T6366 |
25222-25227 |
NN |
denotes |
blood |
T6367 |
25234-25235 |
-LRB- |
denotes |
[ |
T6368 |
25235-25237 |
CD |
denotes |
51 |
T6369 |
25237-25238 |
-RRB- |
denotes |
] |
T6370 |
25238-25239 |
. |
denotes |
. |
T6371 |
25239-25349 |
sentence |
denotes |
In addition, Ca2+ ions can drive spiculation of the membrane bilayer without involving the cytoskeleton [52]. |
T6372 |
25240-25242 |
IN |
denotes |
In |
T6373 |
25267-25272 |
VB |
denotes |
drive |
T6374 |
25243-25251 |
NN |
denotes |
addition |
T6375 |
25251-25253 |
, |
denotes |
, |
T6376 |
25253-25257 |
NN |
denotes |
Ca2+ |
T6377 |
25258-25262 |
NNS |
denotes |
ions |
T6378 |
25263-25266 |
MD |
denotes |
can |
T6379 |
25273-25284 |
NN |
denotes |
spiculation |
T6380 |
25285-25287 |
IN |
denotes |
of |
T6381 |
25288-25291 |
DT |
denotes |
the |
T6382 |
25301-25308 |
NN |
denotes |
bilayer |
T6383 |
25292-25300 |
NN |
denotes |
membrane |
T6384 |
25309-25316 |
IN |
denotes |
without |
T6385 |
25317-25326 |
VBG |
denotes |
involving |
T6386 |
25327-25330 |
DT |
denotes |
the |
T6387 |
25331-25343 |
NN |
denotes |
cytoskeleton |
T6388 |
25344-25345 |
-LRB- |
denotes |
[ |
T6389 |
25345-25347 |
CD |
denotes |
52 |
T6390 |
25347-25348 |
-RRB- |
denotes |
] |
T6391 |
25348-25349 |
. |
denotes |
. |
T6392 |
25349-25427 |
sentence |
denotes |
We have also observed a defect in platelet function in the annexin A7 mutant. |
T6393 |
25350-25352 |
PRP |
denotes |
We |
T6394 |
25363-25371 |
VBN |
denotes |
observed |
T6395 |
25353-25357 |
VBP |
denotes |
have |
T6396 |
25358-25362 |
RB |
denotes |
also |
T6397 |
25372-25373 |
DT |
denotes |
a |
T6398 |
25374-25380 |
NN |
denotes |
defect |
T6399 |
25381-25383 |
IN |
denotes |
in |
T6400 |
25384-25392 |
NN |
denotes |
platelet |
T6401 |
25393-25401 |
NN |
denotes |
function |
T6402 |
25402-25404 |
IN |
denotes |
in |
T6403 |
25405-25408 |
DT |
denotes |
the |
T6404 |
25420-25426 |
NN |
denotes |
mutant |
T6405 |
25409-25416 |
NN |
denotes |
annexin |
T6406 |
25417-25419 |
NN |
denotes |
A7 |
T6407 |
25426-25427 |
. |
denotes |
. |
T6408 |
25427-25571 |
sentence |
denotes |
In the platelet aggregation experiments the initial aggregation velocity was slightly, but significantly lower in platelets lacking annexin A7. |
T6409 |
25428-25430 |
IN |
denotes |
In |
T6410 |
25501-25504 |
VBD |
denotes |
was |
T6411 |
25431-25434 |
DT |
denotes |
the |
T6412 |
25456-25467 |
NNS |
denotes |
experiments |
T6413 |
25435-25443 |
NN |
denotes |
platelet |
T6414 |
25444-25455 |
NN |
denotes |
aggregation |
T6415 |
25468-25471 |
DT |
denotes |
the |
T6416 |
25492-25500 |
NN |
denotes |
velocity |
T6417 |
25472-25479 |
JJ |
denotes |
initial |
T6418 |
25480-25491 |
NN |
denotes |
aggregation |
T6419 |
25505-25513 |
RB |
denotes |
slightly |
T6420 |
25533-25538 |
JJR |
denotes |
lower |
T6421 |
25513-25515 |
, |
denotes |
, |
T6422 |
25515-25518 |
CC |
denotes |
but |
T6423 |
25519-25532 |
RB |
denotes |
significantly |
T6424 |
25539-25541 |
IN |
denotes |
in |
T6425 |
25542-25551 |
NNS |
denotes |
platelets |
T6426 |
25552-25559 |
VBG |
denotes |
lacking |
T6427 |
25560-25567 |
NN |
denotes |
annexin |
T6428 |
25568-25570 |
NN |
denotes |
A7 |
T6429 |
25570-25571 |
. |
denotes |
. |
T6430 |
25571-25642 |
sentence |
denotes |
These data are not reflected by a change in the in vivo bleeding time. |
T6431 |
25572-25577 |
DT |
denotes |
These |
T6432 |
25578-25582 |
NNS |
denotes |
data |
T6433 |
25591-25600 |
VBN |
denotes |
reflected |
T6434 |
25583-25586 |
VBP |
denotes |
are |
T6435 |
25587-25590 |
RB |
denotes |
not |
T6436 |
25601-25603 |
IN |
denotes |
by |
T6437 |
25604-25605 |
DT |
denotes |
a |
T6438 |
25606-25612 |
NN |
denotes |
change |
T6439 |
25613-25615 |
IN |
denotes |
in |
T6440 |
25616-25619 |
DT |
denotes |
the |
T6441 |
25637-25641 |
NN |
denotes |
time |
T6442 |
25620-25622 |
FW |
denotes |
in |
T6443 |
25623-25627 |
FW |
denotes |
vivo |
T6444 |
25628-25636 |
NN |
denotes |
bleeding |
T6445 |
25641-25642 |
. |
denotes |
. |
T6446 |
25642-25813 |
sentence |
denotes |
However the reaction speed of murine platelets is much higher than those of human and due to the technical setup we might have missed larger changes in the mouse samples. |
T6447 |
25643-25650 |
RB |
denotes |
However |
T6448 |
25690-25692 |
VBZ |
denotes |
is |
T6449 |
25651-25654 |
DT |
denotes |
the |
T6450 |
25664-25669 |
NN |
denotes |
speed |
T6451 |
25655-25663 |
NN |
denotes |
reaction |
T6452 |
25670-25672 |
IN |
denotes |
of |
T6453 |
25673-25679 |
JJ |
denotes |
murine |
T6454 |
25680-25689 |
NNS |
denotes |
platelets |
T6455 |
25693-25697 |
RB |
denotes |
much |
T6456 |
25698-25704 |
JJR |
denotes |
higher |
T6457 |
25705-25709 |
IN |
denotes |
than |
T6458 |
25710-25715 |
DT |
denotes |
those |
T6459 |
25716-25718 |
IN |
denotes |
of |
T6460 |
25719-25724 |
JJ |
denotes |
human |
T6461 |
25725-25728 |
CC |
denotes |
and |
T6462 |
25729-25732 |
IN |
denotes |
due |
T6463 |
25770-25776 |
VBN |
denotes |
missed |
T6464 |
25733-25735 |
IN |
denotes |
to |
T6465 |
25736-25739 |
DT |
denotes |
the |
T6466 |
25750-25755 |
NN |
denotes |
setup |
T6467 |
25740-25749 |
JJ |
denotes |
technical |
T6468 |
25756-25758 |
PRP |
denotes |
we |
T6469 |
25759-25764 |
MD |
denotes |
might |
T6470 |
25765-25769 |
VB |
denotes |
have |
T6471 |
25777-25783 |
JJR |
denotes |
larger |
T6472 |
25784-25791 |
NNS |
denotes |
changes |
T6473 |
25792-25794 |
IN |
denotes |
in |
T6474 |
25795-25798 |
DT |
denotes |
the |
T6475 |
25805-25812 |
NNS |
denotes |
samples |
T6476 |
25799-25804 |
NN |
denotes |
mouse |
T6477 |
25812-25813 |
. |
denotes |
. |
T6478 |
25813-25971 |
sentence |
denotes |
The immediate reaction of mouse platelets and the absence of a lag phase after triggering may be caused by an immediately initiated thrombin generation [22]. |
T6479 |
25814-25817 |
DT |
denotes |
The |
T6480 |
25828-25836 |
NN |
denotes |
reaction |
T6481 |
25818-25827 |
JJ |
denotes |
immediate |
T6482 |
25911-25917 |
VBN |
denotes |
caused |
T6483 |
25837-25839 |
IN |
denotes |
of |
T6484 |
25840-25845 |
NN |
denotes |
mouse |
T6485 |
25846-25855 |
NNS |
denotes |
platelets |
T6486 |
25856-25859 |
CC |
denotes |
and |
T6487 |
25860-25863 |
DT |
denotes |
the |
T6488 |
25864-25871 |
NN |
denotes |
absence |
T6489 |
25872-25874 |
IN |
denotes |
of |
T6490 |
25875-25876 |
DT |
denotes |
a |
T6491 |
25881-25886 |
NN |
denotes |
phase |
T6492 |
25877-25880 |
NN |
denotes |
lag |
T6493 |
25887-25892 |
IN |
denotes |
after |
T6494 |
25893-25903 |
NN |
denotes |
triggering |
T6495 |
25904-25907 |
MD |
denotes |
may |
T6496 |
25908-25910 |
VB |
denotes |
be |
T6497 |
25918-25920 |
IN |
denotes |
by |
T6498 |
25921-25923 |
DT |
denotes |
an |
T6499 |
25955-25965 |
NN |
denotes |
generation |
T6500 |
25924-25935 |
RB |
denotes |
immediately |
T6501 |
25936-25945 |
VBN |
denotes |
initiated |
T6502 |
25946-25954 |
NN |
denotes |
thrombin |
T6503 |
25966-25967 |
-LRB- |
denotes |
[ |
T6504 |
25967-25969 |
CD |
denotes |
22 |
T6505 |
25969-25970 |
-RRB- |
denotes |
] |
T6506 |
25970-25971 |
. |
denotes |
. |
T6507 |
25971-26158 |
sentence |
denotes |
As known from the frequent human vWF-syndrome or other haemostatic diseases, small defects often are clinically silent and do not result in physiological changes under normal conditions. |
T6508 |
25972-25974 |
IN |
denotes |
As |
T6509 |
25975-25980 |
VBN |
denotes |
known |
T6510 |
26069-26072 |
VBP |
denotes |
are |
T6511 |
25981-25985 |
IN |
denotes |
from |
T6512 |
25986-25989 |
DT |
denotes |
the |
T6513 |
26009-26017 |
NN |
denotes |
syndrome |
T6514 |
25990-25998 |
JJ |
denotes |
frequent |
T6515 |
25999-26004 |
JJ |
denotes |
human |
T6516 |
26005-26008 |
NN |
denotes |
vWF |
T6517 |
26008-26009 |
HYPH |
denotes |
- |
T6518 |
26018-26020 |
CC |
denotes |
or |
T6519 |
26021-26026 |
JJ |
denotes |
other |
T6520 |
26039-26047 |
NNS |
denotes |
diseases |
T6521 |
26027-26038 |
JJ |
denotes |
haemostatic |
T6522 |
26047-26049 |
, |
denotes |
, |
T6523 |
26049-26054 |
JJ |
denotes |
small |
T6524 |
26055-26062 |
NNS |
denotes |
defects |
T6525 |
26063-26068 |
RB |
denotes |
often |
T6526 |
26073-26083 |
RB |
denotes |
clinically |
T6527 |
26084-26090 |
JJ |
denotes |
silent |
T6528 |
26091-26094 |
CC |
denotes |
and |
T6529 |
26095-26097 |
VBP |
denotes |
do |
T6530 |
26102-26108 |
VB |
denotes |
result |
T6531 |
26098-26101 |
RB |
denotes |
not |
T6532 |
26109-26111 |
IN |
denotes |
in |
T6533 |
26112-26125 |
JJ |
denotes |
physiological |
T6534 |
26126-26133 |
NNS |
denotes |
changes |
T6535 |
26134-26139 |
IN |
denotes |
under |
T6536 |
26140-26146 |
JJ |
denotes |
normal |
T6537 |
26147-26157 |
NNS |
denotes |
conditions |
T6538 |
26157-26158 |
. |
denotes |
. |
T6539 |
26158-26353 |
sentence |
denotes |
Only in situations with severe physical injury, distinct environmental influences or additional platelet attributes like the platelet antigen polymorphism [53] differences might become apparent. |
T6540 |
26159-26163 |
RB |
denotes |
Only |
T6541 |
26164-26166 |
IN |
denotes |
in |
T6542 |
26337-26343 |
VB |
denotes |
become |
T6543 |
26167-26177 |
NNS |
denotes |
situations |
T6544 |
26178-26182 |
IN |
denotes |
with |
T6545 |
26183-26189 |
JJ |
denotes |
severe |
T6546 |
26199-26205 |
NN |
denotes |
injury |
T6547 |
26190-26198 |
JJ |
denotes |
physical |
T6548 |
26205-26207 |
, |
denotes |
, |
T6549 |
26207-26215 |
JJ |
denotes |
distinct |
T6550 |
26230-26240 |
NNS |
denotes |
influences |
T6551 |
26216-26229 |
JJ |
denotes |
environmental |
T6552 |
26241-26243 |
CC |
denotes |
or |
T6553 |
26244-26254 |
JJ |
denotes |
additional |
T6554 |
26255-26263 |
NN |
denotes |
platelet |
T6555 |
26264-26274 |
VBZ |
denotes |
attributes |
T6643 |
26367-26369 |
IN |
denotes |
In |
T6644 |
26384-26390 |
VBP |
denotes |
report |
T6645 |
26370-26374 |
DT |
denotes |
this |
T6646 |
26375-26380 |
NN |
denotes |
paper |
T6647 |
26381-26383 |
PRP |
denotes |
we |
T6648 |
26391-26394 |
DT |
denotes |
the |
T6649 |
26395-26403 |
NN |
denotes |
presence |
T6650 |
26404-26406 |
IN |
denotes |
of |
T6651 |
26407-26411 |
CC |
denotes |
both |
T6652 |
26423-26431 |
NNS |
denotes |
isoforms |
T6653 |
26412-26419 |
NN |
denotes |
annexin |
T6654 |
26420-26422 |
NN |
denotes |
A7 |
T6655 |
26432-26434 |
IN |
denotes |
in |
T6656 |
26435-26438 |
JJ |
denotes |
red |
T6657 |
26445-26450 |
NNS |
denotes |
cells |
T6658 |
26439-26444 |
NN |
denotes |
blood |
T6659 |
26451-26456 |
WRB |
denotes |
where |
T6660 |
26462-26465 |
VBP |
denotes |
are |
T6661 |
26457-26461 |
PRP |
denotes |
they |
T6662 |
26466-26474 |
JJ |
denotes |
abundant |
T6663 |
26475-26477 |
IN |
denotes |
in |
T6664 |
26478-26490 |
NNS |
denotes |
nanovesicles |
T6665 |
26491-26495 |
WDT |
denotes |
that |
T6666 |
26496-26500 |
VBP |
denotes |
form |
T6667 |
26501-26505 |
IN |
denotes |
upon |
T6668 |
26506-26510 |
NN |
denotes |
Ca2+ |
T6669 |
26511-26519 |
NN |
denotes |
addition |
T6670 |
26519-26520 |
. |
denotes |
. |
T6671 |
26520-26588 |
sentence |
denotes |
The proteins are however not required for the vesiculation process. |
T6672 |
26521-26524 |
DT |
denotes |
The |
T6673 |
26525-26533 |
NN |
denotes |
proteins |
T6674 |
26550-26558 |
VBN |
denotes |
required |
T6675 |
26534-26537 |
VBP |
denotes |
are |
T6676 |
26538-26545 |
RB |
denotes |
however |
T6677 |
26546-26549 |
RB |
denotes |
not |
T6678 |
26559-26562 |
IN |
denotes |
for |
T6679 |
26563-26566 |
DT |
denotes |
the |
T6680 |
26580-26587 |
NN |
denotes |
process |
T6681 |
26567-26579 |
NN |
denotes |
vesiculation |
T6682 |
26587-26588 |
. |
denotes |
. |
T6683 |
26588-26852 |
sentence |
denotes |
They are also not essential for the formation of specific membrane domains such as lipid rafts, as exogenously added recombinant annexin A7 redistributed to similar positions in a density gradient containing membranes derived from red blood cells of anxA7-/-mice. |
T6684 |
26589-26593 |
PRP |
denotes |
They |
T6685 |
26594-26597 |
VBP |
denotes |
are |
T6686 |
26598-26602 |
RB |
denotes |
also |
T6687 |
26603-26606 |
RB |
denotes |
not |
T6688 |
26607-26616 |
JJ |
denotes |
essential |
T6689 |
26617-26620 |
IN |
denotes |
for |
T6690 |
26621-26624 |
DT |
denotes |
the |
T6691 |
26625-26634 |
NN |
denotes |
formation |
T6692 |
26635-26637 |
IN |
denotes |
of |
T6693 |
26638-26646 |
JJ |
denotes |
specific |
T6694 |
26656-26663 |
NNS |
denotes |
domains |
T6695 |
26647-26655 |
NN |
denotes |
membrane |
T6696 |
26664-26668 |
JJ |
denotes |
such |
T6697 |
26669-26671 |
IN |
denotes |
as |
T6698 |
26672-26677 |
NN |
denotes |
lipid |
T6699 |
26678-26683 |
NNS |
denotes |
rafts |
T6700 |
26683-26685 |
, |
denotes |
, |
T6701 |
26685-26687 |
IN |
denotes |
as |
T6702 |
26688-26699 |
RB |
denotes |
exogenously |
T6703 |
26700-26705 |
VBN |
denotes |
added |
T6704 |
26726-26728 |
NN |
denotes |
A7 |
T6705 |
26706-26717 |
JJ |
denotes |
recombinant |
T6706 |
26718-26725 |
NN |
denotes |
annexin |
T6707 |
26729-26742 |
VBN |
denotes |
redistributed |
T6708 |
26743-26745 |
IN |
denotes |
to |
T6709 |
26746-26753 |
JJ |
denotes |
similar |
T6710 |
26754-26763 |
NNS |
denotes |
positions |
T6711 |
26764-26766 |
IN |
denotes |
in |
T6712 |
26767-26768 |
DT |
denotes |
a |
T6713 |
26777-26785 |
NN |
denotes |
gradient |
T6714 |
26769-26776 |
NN |
denotes |
density |
T6715 |
26786-26796 |
VBG |
denotes |
containing |
T6716 |
26797-26806 |
NNS |
denotes |
membranes |
T6717 |
26807-26814 |
VBN |
denotes |
derived |
T6718 |
26815-26819 |
IN |
denotes |
from |
T6719 |
26820-26823 |
JJ |
denotes |
red |
T6720 |
26830-26835 |
NNS |
denotes |
cells |
T6721 |
26824-26829 |
NN |
denotes |
blood |
T6722 |
26836-26838 |
IN |
denotes |
of |
T6723 |
26839-26844 |
NN |
denotes |
anxA7 |
T6724 |
26847-26851 |
NNS |
denotes |
mice |
T6725 |
26844-26845 |
SYM |
denotes |
- |
T6726 |
26845-26846 |
HYPH |
denotes |
/ |
T6727 |
26846-26847 |
SYM |
denotes |
- |
T6728 |
26851-26852 |
. |
denotes |
. |
T6729 |
26852-26979 |
sentence |
denotes |
The observed cell shape change of erythrocytes lacking annexin A7 might be due to alterations in the erythrocyte cytoskeleton. |
T6730 |
26853-26856 |
DT |
denotes |
The |
T6731 |
26877-26883 |
NN |
denotes |
change |
T6732 |
26857-26865 |
VBN |
denotes |
observed |
T6733 |
26866-26870 |
NN |
denotes |
cell |
T6734 |
26871-26876 |
NN |
denotes |
shape |
T6735 |
26925-26927 |
VB |
denotes |
be |
T6736 |
26884-26886 |
IN |
denotes |
of |
T6737 |
26887-26899 |
NNS |
denotes |
erythrocytes |
T6738 |
26900-26907 |
VBG |
denotes |
lacking |
T6739 |
26908-26915 |
NN |
denotes |
annexin |
T6740 |
26916-26918 |
NN |
denotes |
A7 |
T6741 |
26919-26924 |
MD |
denotes |
might |
T6742 |
26928-26931 |
IN |
denotes |
due |
T6743 |
26932-26934 |
IN |
denotes |
to |
T6744 |
26935-26946 |
NNS |
denotes |
alterations |
T6745 |
26947-26949 |
IN |
denotes |
in |
T6746 |
26950-26953 |
DT |
denotes |
the |
T6747 |
26966-26978 |
NN |
denotes |
cytoskeleton |
T6748 |
26954-26965 |
NN |
denotes |
erythrocyte |
T6749 |
26978-26979 |
. |
denotes |
. |
T6750 |
26979-27097 |
sentence |
denotes |
As a direct interaction with F-actin was ruled out, this effect might be mediated by annexin's Ca2+-binding activity. |
T6751 |
26980-26982 |
IN |
denotes |
As |
T6752 |
27021-27026 |
VBN |
denotes |
ruled |
T6753 |
26983-26984 |
DT |
denotes |
a |
T6754 |
26992-27003 |
NN |
denotes |
interaction |
T6755 |
26985-26991 |
JJ |
denotes |
direct |
T6756 |
27004-27008 |
IN |
denotes |
with |
T6757 |
27009-27010 |
NN |
denotes |
F |
T6758 |
27011-27016 |
NN |
denotes |
actin |
T6759 |
27010-27011 |
HYPH |
denotes |
- |
T6760 |
27017-27020 |
VBD |
denotes |
was |
T6761 |
27053-27061 |
VBN |
denotes |
mediated |
T6762 |
27027-27030 |
RP |
denotes |
out |
T6763 |
27030-27032 |
, |
denotes |
, |
T6764 |
27032-27036 |
DT |
denotes |
this |
T6765 |
27037-27043 |
NN |
denotes |
effect |
T6766 |
27044-27049 |
MD |
denotes |
might |
T6767 |
27050-27052 |
VB |
denotes |
be |
T6768 |
27062-27064 |
IN |
denotes |
by |
T6769 |
27065-27072 |
NN |
denotes |
annexin |
T6770 |
27088-27096 |
NN |
denotes |
activity |
T6771 |
27072-27074 |
POS |
denotes |
's |
T6772 |
27075-27079 |
NN |
denotes |
Ca2+ |
T6773 |
27080-27087 |
VBG |
denotes |
binding |
T6774 |
27079-27080 |
HYPH |
denotes |
- |
T6775 |
27096-27097 |
. |
denotes |
. |
T6879 |
27108-27119 |
NN |
denotes |
Acquisition |
T6880 |
27120-27122 |
IN |
denotes |
of |
T6881 |
27123-27129 |
JJ |
denotes |
murine |
T6882 |
27140-27145 |
NN |
denotes |
blood |
T6883 |
27130-27133 |
CC |
denotes |
and |
T6884 |
27134-27139 |
JJ |
denotes |
human |
T6885 |
27146-27153 |
NNS |
denotes |
samples |
T6886 |
27153-27441 |
sentence |
denotes |
Blood from 129Sv wild type and annexin A7 knock out mice (anxA7-/-) kept under pathogen-free conditions and fed with regular food, was collected in 1 ml syringes with a 22 gauge needle punctating the right cardiac ventricle immediately after they had been killed by cervical dislocation. |
T6887 |
27154-27159 |
NN |
denotes |
Blood |
T6888 |
27289-27298 |
VBN |
denotes |
collected |
T6889 |
27160-27164 |
IN |
denotes |
from |
T6890 |
27165-27170 |
NN |
denotes |
129Sv |
T6891 |
27206-27210 |
NNS |
denotes |
mice |
T6892 |
27171-27175 |
JJ |
denotes |
wild |
T6893 |
27176-27180 |
NN |
denotes |
type |
T6894 |
27181-27184 |
CC |
denotes |
and |
T6895 |
27185-27192 |
NN |
denotes |
annexin |
T6896 |
27193-27195 |
NN |
denotes |
A7 |
T6897 |
27196-27201 |
NN |
denotes |
knock |
T6898 |
27202-27205 |
NN |
denotes |
out |
T6899 |
27211-27212 |
-LRB- |
denotes |
( |
T6900 |
27212-27217 |
NN |
denotes |
anxA7 |
T6901 |
27217-27218 |
SYM |
denotes |
- |
T6902 |
27218-27219 |
HYPH |
denotes |
/ |
T6903 |
27219-27220 |
SYM |
denotes |
- |
T6904 |
27220-27221 |
-RRB- |
denotes |
) |
T6905 |
27222-27226 |
VBN |
denotes |
kept |
T6906 |
27227-27232 |
IN |
denotes |
under |
T6907 |
27233-27241 |
NN |
denotes |
pathogen |
T6908 |
27242-27246 |
JJ |
denotes |
free |
T6909 |
27241-27242 |
HYPH |
denotes |
- |
T6910 |
27247-27257 |
NNS |
denotes |
conditions |
T6911 |
27258-27261 |
CC |
denotes |
and |
T6912 |
27262-27265 |
VBN |
denotes |
fed |
T6913 |
27266-27270 |
IN |
denotes |
with |
T6914 |
27271-27278 |
JJ |
denotes |
regular |
T6915 |
27279-27283 |
NN |
denotes |
food |
T6916 |
27283-27285 |
, |
denotes |
, |
T6917 |
27285-27288 |
VBD |
denotes |
was |
T6918 |
27299-27301 |
IN |
denotes |
in |
T6919 |
27302-27303 |
CD |
denotes |
1 |
T6920 |
27304-27306 |
NN |
denotes |
ml |
T6921 |
27307-27315 |
NNS |
denotes |
syringes |
T6922 |
27316-27320 |
IN |
denotes |
with |
T6923 |
27321-27322 |
DT |
denotes |
a |
T6924 |
27332-27338 |
NN |
denotes |
needle |
T6925 |
27323-27325 |
CD |
denotes |
22 |
T6926 |
27326-27331 |
NN |
denotes |
gauge |
T6927 |
27339-27349 |
VBG |
denotes |
punctating |
T6928 |
27350-27353 |
DT |
denotes |
the |
T6929 |
27368-27377 |
NN |
denotes |
ventricle |
T6930 |
27354-27359 |
JJ |
denotes |
right |
T6931 |
27360-27367 |
JJ |
denotes |
cardiac |
T6932 |
27378-27389 |
RB |
denotes |
immediately |
T6933 |
27410-27416 |
VBN |
denotes |
killed |
T6934 |
27390-27395 |
IN |
denotes |
after |
T6935 |
27396-27400 |
PRP |
denotes |
they |
T6936 |
27401-27404 |
VBD |
denotes |
had |
T6937 |
27405-27409 |
VBN |
denotes |
been |
T6938 |
27417-27419 |
IN |
denotes |
by |
T6939 |
27420-27428 |
JJ |
denotes |
cervical |
T6940 |
27429-27440 |
NN |
denotes |
dislocation |
T6941 |
27440-27441 |
. |
denotes |
. |
T6942 |
27441-27551 |
sentence |
denotes |
Coagulation generally was inhibited with 1/10 volume of 10 mM NaEDTA, pH 7.5, already present in the syringe. |
T6943 |
27442-27453 |
NN |
denotes |
Coagulation |
T6944 |
27468-27477 |
VBN |
denotes |
inhibited |
T6945 |
27454-27463 |
RB |
denotes |
generally |
T6946 |
27464-27467 |
VBD |
denotes |
was |
T6947 |
27478-27482 |
IN |
denotes |
with |
T6948 |
27483-27484 |
CD |
denotes |
1 |
T6949 |
27485-27487 |
CD |
denotes |
10 |
T6950 |
27484-27485 |
SYM |
denotes |
/ |
T6951 |
27488-27494 |
NN |
denotes |
volume |
T6952 |
27495-27497 |
IN |
denotes |
of |
T6953 |
27498-27500 |
CD |
denotes |
10 |
T6954 |
27501-27503 |
NN |
denotes |
mM |
T6955 |
27504-27510 |
NN |
denotes |
NaEDTA |
T6956 |
27510-27512 |
, |
denotes |
, |
T6957 |
27512-27514 |
NN |
denotes |
pH |
T6958 |
27515-27518 |
CD |
denotes |
7.5 |
T6959 |
27518-27520 |
, |
denotes |
, |
T6960 |
27520-27527 |
RB |
denotes |
already |
T6961 |
27528-27535 |
JJ |
denotes |
present |
T6962 |
27536-27538 |
IN |
denotes |
in |
T6963 |
27539-27542 |
DT |
denotes |
the |
T6964 |
27543-27550 |
NN |
denotes |
syringe |
T6965 |
27550-27551 |
. |
denotes |
. |
T6966 |
27551-27671 |
sentence |
denotes |
The mice were killed by cervical dislocation in order to avoid any effect of anaesthetic chemicals on blood parameters. |
T6967 |
27552-27555 |
DT |
denotes |
The |
T6968 |
27556-27560 |
NNS |
denotes |
mice |
T6969 |
27566-27572 |
VBN |
denotes |
killed |
T6970 |
27561-27565 |
VBD |
denotes |
were |
T6971 |
27573-27575 |
IN |
denotes |
by |
T6972 |
27576-27584 |
JJ |
denotes |
cervical |
T6973 |
27585-27596 |
NN |
denotes |
dislocation |
T6974 |
27597-27599 |
IN |
denotes |
in |
T6975 |
27600-27605 |
NN |
denotes |
order |
T6976 |
27606-27608 |
TO |
denotes |
to |
T6977 |
27609-27614 |
VB |
denotes |
avoid |
T6978 |
27615-27618 |
DT |
denotes |
any |
T6979 |
27619-27625 |
NN |
denotes |
effect |
T6980 |
27626-27628 |
IN |
denotes |
of |
T6981 |
27629-27640 |
JJ |
denotes |
anaesthetic |
T6982 |
27641-27650 |
NNS |
denotes |
chemicals |
T6983 |
27651-27653 |
IN |
denotes |
on |
T6984 |
27654-27659 |
NN |
denotes |
blood |
T6985 |
27660-27670 |
NNS |
denotes |
parameters |
T6986 |
27670-27671 |
. |
denotes |
. |
T6987 |
27671-27751 |
sentence |
denotes |
The wild type and mutant mice were matched regarding their age, weight and sex. |
T6988 |
27672-27675 |
DT |
denotes |
The |
T6989 |
27697-27701 |
NNS |
denotes |
mice |
T6990 |
27676-27680 |
JJ |
denotes |
wild |
T6991 |
27681-27685 |
NN |
denotes |
type |
T6992 |
27686-27689 |
CC |
denotes |
and |
T6993 |
27690-27696 |
JJ |
denotes |
mutant |
T6994 |
27707-27714 |
VBN |
denotes |
matched |
T6995 |
27702-27706 |
VBD |
denotes |
were |
T6996 |
27715-27724 |
VBG |
denotes |
regarding |
T6997 |
27725-27730 |
PRP$ |
denotes |
their |
T6998 |
27731-27734 |
NN |
denotes |
age |
T6999 |
27734-27736 |
, |
denotes |
, |
T7000 |
27736-27742 |
NN |
denotes |
weight |
T7001 |
27743-27746 |
CC |
denotes |
and |
T7002 |
27747-27750 |
NN |
denotes |
sex |
T7003 |
27750-27751 |
. |
denotes |
. |
T7004 |
27751-27863 |
sentence |
denotes |
The daytime of blood collection, sampling site and collection method were kept constant during all experiments. |
T7005 |
27752-27755 |
DT |
denotes |
The |
T7006 |
27756-27763 |
NN |
denotes |
daytime |
T7007 |
27826-27830 |
VBN |
denotes |
kept |
T7008 |
27764-27766 |
IN |
denotes |
of |
T7009 |
27767-27772 |
NN |
denotes |
blood |
T7010 |
27773-27783 |
NN |
denotes |
collection |
T7011 |
27783-27785 |
, |
denotes |
, |
T7012 |
27785-27793 |
VBG |
denotes |
sampling |
T7013 |
27794-27798 |
NN |
denotes |
site |
T7014 |
27799-27802 |
CC |
denotes |
and |
T7015 |
27803-27813 |
NN |
denotes |
collection |
T7016 |
27814-27820 |
NN |
denotes |
method |
T7017 |
27821-27825 |
VBD |
denotes |
were |
T7018 |
27831-27839 |
JJ |
denotes |
constant |
T7019 |
27840-27846 |
IN |
denotes |
during |
T7020 |
27847-27850 |
DT |
denotes |
all |
T7021 |
27851-27862 |
NNS |
denotes |
experiments |
T7022 |
27862-27863 |
. |
denotes |
. |
T7023 |
27863-27968 |
sentence |
denotes |
A typical murine blood sample volume consisted of 550 μl, with variations ranging from 200 μl to 900 μl. |
T7024 |
27864-27865 |
DT |
denotes |
A |
T7025 |
27894-27900 |
NN |
denotes |
volume |
T7026 |
27866-27873 |
JJ |
denotes |
typical |
T7027 |
27874-27880 |
JJ |
denotes |
murine |
T7028 |
27887-27893 |
NN |
denotes |
sample |
T7029 |
27881-27886 |
NN |
denotes |
blood |
T7030 |
27901-27910 |
VBD |
denotes |
consisted |
T7031 |
27911-27913 |
IN |
denotes |
of |
T7032 |
27914-27917 |
CD |
denotes |
550 |
T7033 |
27918-27920 |
NN |
denotes |
μl |
T7034 |
27920-27922 |
, |
denotes |
, |
T7035 |
27922-27926 |
IN |
denotes |
with |
T7036 |
27927-27937 |
NNS |
denotes |
variations |
T7037 |
27938-27945 |
VBG |
denotes |
ranging |
T7038 |
27946-27950 |
IN |
denotes |
from |
T7039 |
27951-27954 |
CD |
denotes |
200 |
T7040 |
27955-27957 |
NNS |
denotes |
μl |
T7041 |
27958-27960 |
IN |
denotes |
to |
T7042 |
27961-27964 |
CD |
denotes |
900 |
T7043 |
27965-27967 |
NNS |
denotes |
μl |
T7044 |
27967-27968 |
. |
denotes |
. |
T7045 |
27968-28078 |
sentence |
denotes |
Human blood samples from the authors were collected by vein puncture using common EDTA-monovettes (Sarstedt). |
T7046 |
27969-27974 |
JJ |
denotes |
Human |
T7047 |
27981-27988 |
NNS |
denotes |
samples |
T7048 |
27975-27980 |
NN |
denotes |
blood |
T7049 |
28011-28020 |
VBN |
denotes |
collected |
T7050 |
27989-27993 |
IN |
denotes |
from |
T7051 |
27994-27997 |
DT |
denotes |
the |
T7052 |
27998-28005 |
NNS |
denotes |
authors |
T7053 |
28006-28010 |
VBD |
denotes |
were |
T7054 |
28021-28023 |
IN |
denotes |
by |
T7055 |
28024-28028 |
NN |
denotes |
vein |
T7056 |
28029-28037 |
NN |
denotes |
puncture |
T7057 |
28038-28043 |
VBG |
denotes |
using |
T7058 |
28044-28050 |
JJ |
denotes |
common |
T7059 |
28056-28066 |
NNS |
denotes |
monovettes |
T7060 |
28051-28055 |
NN |
denotes |
EDTA |
T7061 |
28055-28056 |
HYPH |
denotes |
- |
T7062 |
28067-28068 |
-LRB- |
denotes |
( |
T7063 |
28068-28076 |
NNP |
denotes |
Sarstedt |
T7064 |
28076-28077 |
-RRB- |
denotes |
) |
T7065 |
28077-28078 |
. |
denotes |
. |
T7066 |
28078-28271 |
sentence |
denotes |
We performed our studies using well standardized sampling conditions, as differences in the blood sampling site, for example between tail and heart, show large changes in all cell type counts. |
T7067 |
28079-28081 |
PRP |
denotes |
We |
T7068 |
28082-28091 |
VBD |
denotes |
performed |
T7069 |
28092-28095 |
PRP$ |
denotes |
our |
T7070 |
28096-28103 |
NNS |
denotes |
studies |
T7071 |
28104-28109 |
VBG |
denotes |
using |
T7072 |
28110-28114 |
RB |
denotes |
well |
T7073 |
28115-28127 |
JJ |
denotes |
standardized |
T7074 |
28137-28147 |
NNS |
denotes |
conditions |
T7075 |
28128-28136 |
NN |
denotes |
sampling |
T7076 |
28147-28149 |
, |
denotes |
, |
T7077 |
28149-28151 |
IN |
denotes |
as |
T7078 |
28228-28232 |
VBP |
denotes |
show |
T7079 |
28152-28163 |
NNS |
denotes |
differences |
T7080 |
28164-28166 |
IN |
denotes |
in |
T7081 |
28167-28170 |
DT |
denotes |
the |
T7082 |
28186-28190 |
NN |
denotes |
site |
T7083 |
28171-28176 |
NN |
denotes |
blood |
T7084 |
28177-28185 |
NN |
denotes |
sampling |
T7085 |
28190-28192 |
, |
denotes |
, |
T7086 |
28192-28195 |
IN |
denotes |
for |
T7087 |
28204-28211 |
IN |
denotes |
between |
T7088 |
28196-28203 |
NN |
denotes |
example |
T7089 |
28212-28216 |
NN |
denotes |
tail |
T7090 |
28217-28220 |
CC |
denotes |
and |
T7091 |
28221-28226 |
NN |
denotes |
heart |
T7092 |
28226-28228 |
, |
denotes |
, |
T7093 |
28233-28238 |
JJ |
denotes |
large |
T7094 |
28239-28246 |
NNS |
denotes |
changes |
T7095 |
28247-28249 |
IN |
denotes |
in |
T7096 |
28250-28253 |
DT |
denotes |
all |
T7097 |
28264-28270 |
NNS |
denotes |
counts |
T7098 |
28254-28258 |
NN |
denotes |
cell |
T7099 |
28259-28263 |
NN |
denotes |
type |
T7100 |
28270-28271 |
. |
denotes |
. |
T7101 |
28271-28337 |
sentence |
denotes |
First and second sample draw affect blood measurements, too [22]. |
T7102 |
28272-28277 |
JJ |
denotes |
First |
T7103 |
28296-28300 |
NN |
denotes |
draw |
T7104 |
28278-28281 |
CC |
denotes |
and |
T7105 |
28282-28288 |
JJ |
denotes |
second |
T7106 |
28289-28295 |
NN |
denotes |
sample |
T7107 |
28301-28307 |
VBP |
denotes |
affect |
T7108 |
28308-28313 |
NN |
denotes |
blood |
T7109 |
28314-28326 |
NNS |
denotes |
measurements |
T7110 |
28326-28328 |
, |
denotes |
, |
T7111 |
28328-28331 |
RB |
denotes |
too |
T7112 |
28332-28333 |
-LRB- |
denotes |
[ |
T7113 |
28333-28335 |
CD |
denotes |
22 |
T7114 |
28335-28336 |
-RRB- |
denotes |
] |
T7115 |
28336-28337 |
. |
denotes |
. |
T7116 |
28337-28429 |
sentence |
denotes |
Also the genetic background in common laboratory and transgenic mice affects the phenotype. |
T7117 |
28338-28342 |
RB |
denotes |
Also |
T7118 |
28407-28414 |
VBZ |
denotes |
affects |
T7119 |
28343-28346 |
DT |
denotes |
the |
T7120 |
28355-28365 |
NN |
denotes |
background |
T7121 |
28347-28354 |
JJ |
denotes |
genetic |
T7122 |
28366-28368 |
IN |
denotes |
in |
T7123 |
28369-28375 |
JJ |
denotes |
common |
T7124 |
28402-28406 |
NNS |
denotes |
mice |
T7125 |
28376-28386 |
NN |
denotes |
laboratory |
T7126 |
28387-28390 |
CC |
denotes |
and |
T7127 |
28391-28401 |
JJ |
denotes |
transgenic |
T7128 |
28415-28418 |
DT |
denotes |
the |
T7129 |
28419-28428 |
NN |
denotes |
phenotype |
T7130 |
28428-28429 |
. |
denotes |
. |
T7131 |
28429-28536 |
sentence |
denotes |
A change from one strain to another may protect mice from effects of the primarily induced genetic defect. |
T7132 |
28430-28431 |
DT |
denotes |
A |
T7133 |
28432-28438 |
NN |
denotes |
change |
T7134 |
28470-28477 |
VB |
denotes |
protect |
T7135 |
28439-28443 |
IN |
denotes |
from |
T7136 |
28444-28447 |
CD |
denotes |
one |
T7137 |
28448-28454 |
NN |
denotes |
strain |
T7138 |
28455-28457 |
IN |
denotes |
to |
T7139 |
28458-28465 |
DT |
denotes |
another |
T7140 |
28466-28469 |
MD |
denotes |
may |
T7141 |
28478-28482 |
NNS |
denotes |
mice |
T7142 |
28483-28487 |
IN |
denotes |
from |
T7143 |
28488-28495 |
NNS |
denotes |
effects |
T7144 |
28496-28498 |
IN |
denotes |
of |
T7145 |
28499-28502 |
DT |
denotes |
the |
T7146 |
28529-28535 |
NN |
denotes |
defect |
T7147 |
28503-28512 |
RB |
denotes |
primarily |
T7148 |
28513-28520 |
VBN |
denotes |
induced |
T7149 |
28521-28528 |
JJ |
denotes |
genetic |
T7150 |
28535-28536 |
. |
denotes |
. |
T7151 |
28536-28696 |
sentence |
denotes |
For example, BALB/c mice have a significantly increased velocity of platelet aggregation as compared to 129Sv mice, which were used throughout this study [23]. |
T7152 |
28537-28540 |
IN |
denotes |
For |
T7153 |
28562-28566 |
VBP |
denotes |
have |
T7154 |
28541-28548 |
NN |
denotes |
example |
T7155 |
28548-28550 |
, |
denotes |
, |
T7156 |
28550-28554 |
NN |
denotes |
BALB |
T7157 |
28555-28556 |
NN |
denotes |
c |
T7158 |
28554-28555 |
HYPH |
denotes |
/ |
T7159 |
28557-28561 |
NNS |
denotes |
mice |
T7160 |
28567-28568 |
DT |
denotes |
a |
T7161 |
28593-28601 |
NN |
denotes |
velocity |
T7162 |
28569-28582 |
RB |
denotes |
significantly |
T7163 |
28583-28592 |
VBN |
denotes |
increased |
T7164 |
28602-28604 |
IN |
denotes |
of |
T7165 |
28605-28613 |
NN |
denotes |
platelet |
T7166 |
28614-28625 |
NN |
denotes |
aggregation |
T7167 |
28626-28628 |
IN |
denotes |
as |
T7168 |
28629-28637 |
VBN |
denotes |
compared |
T7169 |
28638-28640 |
IN |
denotes |
to |
T7170 |
28641-28646 |
NN |
denotes |
129Sv |
T7171 |
28647-28651 |
NNS |
denotes |
mice |
T7172 |
28651-28653 |
, |
denotes |
, |
T7173 |
28653-28658 |
WDT |
denotes |
which |
T7174 |
28664-28668 |
VBN |
denotes |
used |
T7175 |
28659-28663 |
VBD |
denotes |
were |
T7176 |
28669-28679 |
IN |
denotes |
throughout |
T7177 |
28680-28684 |
DT |
denotes |
this |
T7178 |
28685-28690 |
NN |
denotes |
study |
T7179 |
28691-28692 |
-LRB- |
denotes |
[ |
T7180 |
28692-28694 |
CD |
denotes |
23 |
T7181 |
28694-28695 |
-RRB- |
denotes |
] |
T7182 |
28695-28696 |
. |
denotes |
. |
T7273 |
28698-28702 |
NN |
denotes |
Self |
T7274 |
28703-28710 |
VBG |
denotes |
forming |
T7275 |
28729-28738 |
NNS |
denotes |
gradients |
T7276 |
28711-28720 |
NN |
denotes |
iodixanol |
T7277 |
28721-28728 |
NN |
denotes |
density |
T7278 |
28739-28741 |
IN |
denotes |
of |
T7279 |
28742-28747 |
JJ |
denotes |
human |
T7280 |
28758-28763 |
NNS |
denotes |
cells |
T7281 |
28748-28751 |
JJ |
denotes |
red |
T7282 |
28752-28757 |
NN |
denotes |
blood |
T7283 |
28763-28839 |
sentence |
denotes |
The subcellular distribution of annexin A7 was addressed according to [24]. |
T7284 |
28764-28767 |
DT |
denotes |
The |
T7285 |
28780-28792 |
NN |
denotes |
distribution |
T7286 |
28768-28779 |
JJ |
denotes |
subcellular |
T7287 |
28811-28820 |
VBN |
denotes |
addressed |
T7288 |
28793-28795 |
IN |
denotes |
of |
T7289 |
28796-28803 |
NN |
denotes |
annexin |
T7290 |
28804-28806 |
NN |
denotes |
A7 |
T7291 |
28807-28810 |
VBD |
denotes |
was |
T7292 |
28821-28830 |
VBG |
denotes |
according |
T7293 |
28831-28833 |
IN |
denotes |
to |
T7294 |
28834-28835 |
-LRB- |
denotes |
[ |
T7295 |
28835-28837 |
CD |
denotes |
24 |
T7296 |
28837-28838 |
-RRB- |
denotes |
] |
T7297 |
28838-28839 |
. |
denotes |
. |
T7298 |
28839-29145 |
sentence |
denotes |
Red blood cells were centrifuged and washed in isotonic buffered salt solution, collected in ice cold lysis buffer (20 mM Hepes, pH 7.4, 200 μM CaCl2, 0.25 M sucrose, and proteinase inhibitors (Roche)), homogenized by a loosely fitting dounce homogenizer and additional passages through a 22 gauge needle. |
T7299 |
28840-28843 |
JJ |
denotes |
Red |
T7300 |
28850-28855 |
NNS |
denotes |
cells |
T7301 |
28844-28849 |
NN |
denotes |
blood |
T7302 |
28861-28872 |
VBN |
denotes |
centrifuged |
T7303 |
28856-28860 |
VBD |
denotes |
were |
T7304 |
28873-28876 |
CC |
denotes |
and |
T7305 |
28877-28883 |
VBN |
denotes |
washed |
T7306 |
28884-28886 |
IN |
denotes |
in |
T7307 |
28887-28895 |
JJ |
denotes |
isotonic |
T7308 |
28910-28918 |
NN |
denotes |
solution |
T7309 |
28896-28904 |
VBN |
denotes |
buffered |
T7310 |
28905-28909 |
NN |
denotes |
salt |
T7311 |
28918-28920 |
, |
denotes |
, |
T7312 |
28920-28929 |
VBN |
denotes |
collected |
T7313 |
28930-28932 |
IN |
denotes |
in |
T7314 |
28933-28936 |
NN |
denotes |
ice |
T7315 |
28937-28941 |
JJ |
denotes |
cold |
T7316 |
28948-28954 |
NN |
denotes |
buffer |
T7317 |
28942-28947 |
NN |
denotes |
lysis |
T7318 |
28955-28956 |
-LRB- |
denotes |
( |
T7319 |
28962-28967 |
NN |
denotes |
Hepes |
T7320 |
28956-28958 |
CD |
denotes |
20 |
T7321 |
28959-28961 |
NN |
denotes |
mM |
T7322 |
28967-28969 |
, |
denotes |
, |
T7323 |
28969-28971 |
NN |
denotes |
pH |
T7324 |
28972-28975 |
CD |
denotes |
7.4 |
T7325 |
28975-28977 |
, |
denotes |
, |
T7326 |
28977-28980 |
CD |
denotes |
200 |
T7327 |
28981-28983 |
NN |
denotes |
μM |
T7328 |
28984-28989 |
NN |
denotes |
CaCl2 |
T7329 |
28989-28991 |
, |
denotes |
, |
T7330 |
28991-28995 |
CD |
denotes |
0.25 |
T7331 |
28996-28997 |
NN |
denotes |
M |
T7332 |
28998-29005 |
NN |
denotes |
sucrose |
T7333 |
29005-29007 |
, |
denotes |
, |
T7334 |
29007-29010 |
CC |
denotes |
and |
T7335 |
29011-29021 |
NN |
denotes |
proteinase |
T7336 |
29022-29032 |
NNS |
denotes |
inhibitors |
T7337 |
29033-29034 |
-LRB- |
denotes |
( |
T7338 |
29034-29039 |
NNP |
denotes |
Roche |
T7339 |
29039-29040 |
-RRB- |
denotes |
) |
T7340 |
29040-29041 |
-RRB- |
denotes |
) |
T7341 |
29041-29043 |
, |
denotes |
, |
T7342 |
29043-29054 |
VBN |
denotes |
homogenized |
T7343 |
29055-29057 |
IN |
denotes |
by |
T7344 |
29058-29059 |
DT |
denotes |
a |
T7345 |
29083-29094 |
NN |
denotes |
homogenizer |
T7346 |
29060-29067 |
RB |
denotes |
loosely |
T7347 |
29068-29075 |
JJ |
denotes |
fitting |
T7348 |
29076-29082 |
NN |
denotes |
dounce |
T7349 |
29095-29098 |
CC |
denotes |
and |
T7350 |
29099-29109 |
JJ |
denotes |
additional |
T7351 |
29110-29118 |
NNS |
denotes |
passages |
T7352 |
29119-29126 |
IN |
denotes |
through |
T7353 |
29127-29128 |
DT |
denotes |
a |
T7354 |
29138-29144 |
NN |
denotes |
needle |
T7355 |
29129-29131 |
CD |
denotes |
22 |
T7356 |
29132-29137 |
NN |
denotes |
gauge |
T7357 |
29144-29145 |
. |
denotes |
. |
T7358 |
29145-29305 |
sentence |
denotes |
A 50 % iodixanol solution was established by mixing Optiprep solution (60 % iodixanol; Sigma) with buffer (120 mM Hepes, pH 7.4, 1.2 mM CaCl2, 0.25 M sucrose). |
T7359 |
29146-29147 |
DT |
denotes |
A |
T7360 |
29163-29171 |
NN |
denotes |
solution |
T7361 |
29148-29150 |
CD |
denotes |
50 |
T7362 |
29151-29152 |
NN |
denotes |
% |
T7363 |
29153-29162 |
NN |
denotes |
iodixanol |
T7364 |
29176-29187 |
VBN |
denotes |
established |
T7365 |
29172-29175 |
VBD |
denotes |
was |
T7366 |
29188-29190 |
IN |
denotes |
by |
T7367 |
29191-29197 |
VBG |
denotes |
mixing |
T7368 |
29198-29206 |
NN |
denotes |
Optiprep |
T7369 |
29207-29215 |
NN |
denotes |
solution |
T7370 |
29216-29217 |
-LRB- |
denotes |
( |
T7371 |
29233-29238 |
NN |
denotes |
Sigma |
T7372 |
29217-29219 |
CD |
denotes |
60 |
T7373 |
29220-29221 |
NN |
denotes |
% |
T7374 |
29222-29231 |
NN |
denotes |
iodixanol |
T7375 |
29231-29232 |
: |
denotes |
; |
T7376 |
29238-29239 |
-RRB- |
denotes |
) |
T7377 |
29240-29244 |
IN |
denotes |
with |
T7378 |
29245-29251 |
NN |
denotes |
buffer |
T7379 |
29252-29253 |
-LRB- |
denotes |
( |
T7380 |
29296-29303 |
NN |
denotes |
sucrose |
T7381 |
29253-29256 |
CD |
denotes |
120 |
T7382 |
29257-29259 |
NN |
denotes |
mM |
T7383 |
29260-29265 |
NN |
denotes |
Hepes |
T7384 |
29265-29267 |
, |
denotes |
, |
T7385 |
29267-29269 |
NN |
denotes |
pH |
T7386 |
29270-29273 |
CD |
denotes |
7.4 |
T7387 |
29273-29275 |
, |
denotes |
, |
T7388 |
29275-29278 |
CD |
denotes |
1.2 |
T7389 |
29279-29281 |
NN |
denotes |
mM |
T7390 |
29282-29287 |
NN |
denotes |
CaCl2 |
T7391 |
29287-29289 |
, |
denotes |
, |
T7392 |
29289-29293 |
CD |
denotes |
0.25 |
T7393 |
29294-29295 |
NN |
denotes |
M |
T7394 |
29303-29304 |
-RRB- |
denotes |
) |
T7395 |
29304-29305 |
. |
denotes |
. |
T7396 |
29305-29472 |
sentence |
denotes |
Seven parts of this 50 % iodixanol solution were mixed with thirteen parts of the red blood cell solution, generating a gradient solution containing 17.5 % iodixanol. |
T7397 |
29306-29311 |
CD |
denotes |
Seven |
T7398 |
29312-29317 |
NNS |
denotes |
parts |
T7399 |
29355-29360 |
VBN |
denotes |
mixed |
T7400 |
29318-29320 |
IN |
denotes |
of |
T7401 |
29321-29325 |
DT |
denotes |
this |
T7402 |
29341-29349 |
NN |
denotes |
solution |
T7403 |
29326-29328 |
CD |
denotes |
50 |
T7404 |
29329-29330 |
NN |
denotes |
% |
T7405 |
29331-29340 |
NN |
denotes |
iodixanol |
T7406 |
29350-29354 |
VBD |
denotes |
were |
T7407 |
29361-29365 |
IN |
denotes |
with |
T7408 |
29366-29374 |
CD |
denotes |
thirteen |
T7409 |
29375-29380 |
NNS |
denotes |
parts |
T7410 |
29381-29383 |
IN |
denotes |
of |
T7411 |
29384-29387 |
DT |
denotes |
the |
T7412 |
29403-29411 |
NN |
denotes |
solution |
T7413 |
29388-29391 |
JJ |
denotes |
red |
T7414 |
29398-29402 |
NN |
denotes |
cell |
T7415 |
29392-29397 |
NN |
denotes |
blood |
T7416 |
29411-29413 |
, |
denotes |
, |
T7417 |
29413-29423 |
VBG |
denotes |
generating |
T7418 |
29424-29425 |
DT |
denotes |
a |
T7419 |
29435-29443 |
NN |
denotes |
solution |
T7420 |
29426-29434 |
NN |
denotes |
gradient |
T7421 |
29444-29454 |
VBG |
denotes |
containing |
T7422 |
29455-29459 |
CD |
denotes |
17.5 |
T7423 |
29460-29461 |
NN |
denotes |
% |
T7424 |
29462-29471 |
NN |
denotes |
iodixanol |
T7425 |
29471-29472 |
. |
denotes |
. |
T7426 |
29472-29739 |
sentence |
denotes |
The mixture was filled into a centrifuge tube underlayed by cushions of 30 % and 35 % iodixanol and centrifuged for 3 hours at 270,000 × g in a SW41 Ti swing out rotor (Beckman) to achieve a nearly linear density gradient (approximately 1.06 g/ml to 1.20 g/ml) [24]. |
T7427 |
29473-29476 |
DT |
denotes |
The |
T7428 |
29477-29484 |
NN |
denotes |
mixture |
T7429 |
29489-29495 |
VBN |
denotes |
filled |
T7430 |
29485-29488 |
VBD |
denotes |
was |
T7431 |
29496-29500 |
IN |
denotes |
into |
T7432 |
29501-29502 |
DT |
denotes |
a |
T7433 |
29514-29518 |
NN |
denotes |
tube |
T7434 |
29503-29513 |
JJ |
denotes |
centrifuge |
T7435 |
29519-29529 |
VBN |
denotes |
underlayed |
T7436 |
29530-29532 |
IN |
denotes |
by |
T7437 |
29533-29541 |
NNS |
denotes |
cushions |
T7438 |
29542-29544 |
IN |
denotes |
of |
T7439 |
29545-29547 |
CD |
denotes |
30 |
T7440 |
29548-29549 |
NN |
denotes |
% |
T7441 |
29559-29568 |
NN |
denotes |
iodixanol |
T7442 |
29550-29553 |
CC |
denotes |
and |
T7443 |
29554-29556 |
CD |
denotes |
35 |
T7444 |
29557-29558 |
NN |
denotes |
% |
T7445 |
29569-29572 |
CC |
denotes |
and |
T7446 |
29573-29584 |
VBN |
denotes |
centrifuged |
T7447 |
29585-29588 |
IN |
denotes |
for |
T7448 |
29589-29590 |
CD |
denotes |
3 |
T7449 |
29591-29596 |
NNS |
denotes |
hours |
T7450 |
29597-29599 |
IN |
denotes |
at |
T7451 |
29600-29607 |
CD |
denotes |
270,000 |
T7452 |
29610-29611 |
NN |
denotes |
g |
T7453 |
29608-29609 |
SYM |
denotes |
× |
T7454 |
29612-29614 |
IN |
denotes |
in |
T7455 |
29615-29616 |
DT |
denotes |
a |
T7456 |
29635-29640 |
NN |
denotes |
rotor |
T7457 |
29617-29621 |
NN |
denotes |
SW41 |
T7458 |
29622-29624 |
NNP |
denotes |
Ti |
T7459 |
29625-29630 |
NN |
denotes |
swing |
T7460 |
29631-29634 |
NN |
denotes |
out |
T7461 |
29641-29642 |
-LRB- |
denotes |
( |
T7462 |
29642-29649 |
NN |
denotes |
Beckman |
T7463 |
29649-29650 |
-RRB- |
denotes |
) |
T7464 |
29651-29653 |
TO |
denotes |
to |
T7465 |
29654-29661 |
VB |
denotes |
achieve |
T7466 |
29662-29663 |
DT |
denotes |
a |
T7467 |
29686-29694 |
NN |
denotes |
gradient |
T7468 |
29664-29670 |
RB |
denotes |
nearly |
T7469 |
29671-29677 |
JJ |
denotes |
linear |
T7470 |
29678-29685 |
NN |
denotes |
density |
T7471 |
29695-29696 |
-LRB- |
denotes |
( |
T7472 |
29715-29716 |
NNS |
denotes |
g |
T7473 |
29696-29709 |
RB |
denotes |
approximately |
T7474 |
29710-29714 |
CD |
denotes |
1.06 |
T7475 |
29716-29717 |
SYM |
denotes |
/ |
T7476 |
29717-29719 |
NN |
denotes |
ml |
T7477 |
29720-29722 |
IN |
denotes |
to |
T7478 |
29723-29727 |
CD |
denotes |
1.20 |
T7479 |
29728-29729 |
NNS |
denotes |
g |
T7480 |
29729-29730 |
SYM |
denotes |
/ |
T7481 |
29730-29732 |
NN |
denotes |
ml |
T7482 |
29732-29733 |
-RRB- |
denotes |
) |
T7483 |
29734-29735 |
-LRB- |
denotes |
[ |
T7484 |
29735-29737 |
CD |
denotes |
24 |
T7485 |
29737-29738 |
-RRB- |
denotes |
] |
T7486 |
29738-29739 |
. |
denotes |
. |
T7487 |
29739-29903 |
sentence |
denotes |
Fractions of 0.6 ml were collected from the top of the gradient by a 1 ml syringe without using a needle and immediately frozen in liquid nitrogen for further use. |
T7488 |
29740-29749 |
NNS |
denotes |
Fractions |
T7489 |
29765-29774 |
VBN |
denotes |
collected |
T7490 |
29750-29752 |
IN |
denotes |
of |
T7491 |
29753-29756 |
CD |
denotes |
0.6 |
T7492 |
29757-29759 |
NN |
denotes |
ml |
T7493 |
29760-29764 |
VBD |
denotes |
were |
T7494 |
29775-29779 |
IN |
denotes |
from |
T7495 |
29780-29783 |
DT |
denotes |
the |
T7496 |
29784-29787 |
NN |
denotes |
top |
T7497 |
29788-29790 |
IN |
denotes |
of |
T7498 |
29791-29794 |
DT |
denotes |
the |
T7499 |
29795-29803 |
NN |
denotes |
gradient |
T7500 |
29804-29806 |
IN |
denotes |
by |
T7501 |
29807-29808 |
DT |
denotes |
a |
T7502 |
29814-29821 |
NN |
denotes |
syringe |
T7503 |
29809-29810 |
CD |
denotes |
1 |
T7504 |
29811-29813 |
NN |
denotes |
ml |
T7505 |
29822-29829 |
IN |
denotes |
without |
T7506 |
29830-29835 |
VBG |
denotes |
using |
T7507 |
29836-29837 |
DT |
denotes |
a |
T7508 |
29838-29844 |
NN |
denotes |
needle |
T7509 |
29845-29848 |
CC |
denotes |
and |
T7510 |
29849-29860 |
RB |
denotes |
immediately |
T7511 |
29861-29867 |
VBN |
denotes |
frozen |
T7512 |
29868-29870 |
IN |
denotes |
in |
T7513 |
29871-29877 |
JJ |
denotes |
liquid |
T7514 |
29878-29886 |
NN |
denotes |
nitrogen |
T7515 |
29887-29890 |
IN |
denotes |
for |
T7516 |
29891-29898 |
JJ |
denotes |
further |
T7517 |
29899-29902 |
NN |
denotes |
use |
T7518 |
29902-29903 |
. |
denotes |
. |
T7632 |
29905-29915 |
NN |
denotes |
Exovesicle |
T7633 |
29916-29926 |
NN |
denotes |
generation |
T7634 |
29927-29931 |
IN |
denotes |
from |
T7635 |
29932-29937 |
JJ |
denotes |
human |
T7636 |
29959-29964 |
NNS |
denotes |
cells |
T7637 |
29938-29941 |
CC |
denotes |
and |
T7638 |
29942-29948 |
JJ |
denotes |
murine |
T7639 |
29949-29952 |
JJ |
denotes |
red |
T7640 |
29953-29958 |
NN |
denotes |
blood |
T7641 |
29964-30034 |
sentence |
denotes |
Red blood cells naturally can form hemoglobin containing exovesicles. |
T7642 |
29965-29968 |
JJ |
denotes |
Red |
T7643 |
29975-29980 |
NNS |
denotes |
cells |
T7644 |
29969-29974 |
NN |
denotes |
blood |
T7645 |
29995-29999 |
VB |
denotes |
form |
T7646 |
29981-29990 |
RB |
denotes |
naturally |
T7647 |
29991-29994 |
MD |
denotes |
can |
T7648 |
30000-30010 |
NN |
denotes |
hemoglobin |
T7649 |
30011-30021 |
VBG |
denotes |
containing |
T7650 |
30022-30033 |
NNS |
denotes |
exovesicles |
T7651 |
30033-30034 |
. |
denotes |
. |
T7652 |
30034-30084 |
sentence |
denotes |
In vitro these vesicle formation is Ca2+ induced. |
T7653 |
30035-30037 |
FW |
denotes |
In |
T7654 |
30038-30043 |
FW |
denotes |
vitro |
T7655 |
30068-30070 |
VBZ |
denotes |
is |
T7656 |
30044-30049 |
DT |
denotes |
these |
T7657 |
30058-30067 |
NN |
denotes |
formation |
T7658 |
30050-30057 |
NN |
denotes |
vesicle |
T7659 |
30071-30075 |
NN |
denotes |
Ca2+ |
T7660 |
30076-30083 |
VBN |
denotes |
induced |
T7661 |
30083-30084 |
. |
denotes |
. |
T7662 |
30084-30198 |
sentence |
denotes |
Freshly collected lithium-heparin anticoagulated blood samples were centrifuged for 5 minutes at 400 × g and 4°C. |
T7663 |
30085-30092 |
RB |
denotes |
Freshly |
T7664 |
30093-30102 |
VBN |
denotes |
collected |
T7665 |
30140-30147 |
NNS |
denotes |
samples |
T7666 |
30103-30110 |
NN |
denotes |
lithium |
T7667 |
30111-30118 |
NN |
denotes |
heparin |
T7668 |
30110-30111 |
HYPH |
denotes |
- |
T7669 |
30119-30133 |
VBN |
denotes |
anticoagulated |
T7670 |
30134-30139 |
NN |
denotes |
blood |
T7671 |
30153-30164 |
VBN |
denotes |
centrifuged |
T7672 |
30148-30152 |
VBD |
denotes |
were |
T7673 |
30165-30168 |
IN |
denotes |
for |
T7674 |
30169-30170 |
CD |
denotes |
5 |
T7675 |
30171-30178 |
NNS |
denotes |
minutes |
T7676 |
30179-30181 |
IN |
denotes |
at |
T7677 |
30182-30185 |
CD |
denotes |
400 |
T7678 |
30188-30189 |
NN |
denotes |
g |
T7679 |
30186-30187 |
SYM |
denotes |
× |
T7680 |
30190-30193 |
CC |
denotes |
and |
T7681 |
30194-30195 |
CD |
denotes |
4 |
T7682 |
30195-30197 |
NN |
denotes |
°C |
T7683 |
30197-30198 |
. |
denotes |
. |
T7684 |
30198-30298 |
sentence |
denotes |
The erythrocytes were washed five times with 20 mM Hepes, pH 7.5, and 150 mM NaCl (washing buffer). |
T7685 |
30199-30202 |
DT |
denotes |
The |
T7686 |
30203-30215 |
NNS |
denotes |
erythrocytes |
T7687 |
30221-30227 |
VBN |
denotes |
washed |
T7688 |
30216-30220 |
VBD |
denotes |
were |
T7689 |
30228-30232 |
CD |
denotes |
five |
T7690 |
30233-30238 |
NNS |
denotes |
times |
T7691 |
30239-30243 |
IN |
denotes |
with |
T7692 |
30244-30246 |
CD |
denotes |
20 |
T7693 |
30247-30249 |
NN |
denotes |
mM |
T7694 |
30250-30255 |
NN |
denotes |
Hepes |
T7695 |
30255-30257 |
, |
denotes |
, |
T7696 |
30257-30259 |
NN |
denotes |
pH |
T7697 |
30260-30263 |
CD |
denotes |
7.5 |
T7698 |
30263-30265 |
, |
denotes |
, |
T7699 |
30265-30268 |
CC |
denotes |
and |
T7700 |
30269-30272 |
CD |
denotes |
150 |
T7701 |
30273-30275 |
NN |
denotes |
mM |
T7702 |
30276-30280 |
NN |
denotes |
NaCl |
T7703 |
30281-30282 |
-LRB- |
denotes |
( |
T7704 |
30290-30296 |
NN |
denotes |
buffer |
T7705 |
30282-30289 |
VBG |
denotes |
washing |
T7706 |
30296-30297 |
-RRB- |
denotes |
) |
T7707 |
30297-30298 |
. |
denotes |
. |
T7708 |
30298-30511 |
sentence |
denotes |
A volume of 500 μl washed red blood cells were combined with 3 ml of vesiculation buffer (20 mM Hepes, pH 7.5, 150 mM NaCl, 1 mM CaCl2 adjusted to 5 μM Ca2+ ionophore A23187) and incubated for 30 minutes at 37°C. |
T7709 |
30299-30300 |
DT |
denotes |
A |
T7710 |
30301-30307 |
NN |
denotes |
volume |
T7711 |
30346-30354 |
VBN |
denotes |
combined |
T7712 |
30308-30310 |
IN |
denotes |
of |
T7713 |
30311-30314 |
CD |
denotes |
500 |
T7714 |
30315-30317 |
NN |
denotes |
μl |
T7715 |
30335-30340 |
NNS |
denotes |
cells |
T7716 |
30318-30324 |
VBN |
denotes |
washed |
T7717 |
30325-30328 |
JJ |
denotes |
red |
T7718 |
30329-30334 |
NN |
denotes |
blood |
T7719 |
30341-30345 |
VBD |
denotes |
were |
T7720 |
30355-30359 |
IN |
denotes |
with |
T7721 |
30360-30361 |
CD |
denotes |
3 |
T7722 |
30362-30364 |
NN |
denotes |
ml |
T7723 |
30365-30367 |
IN |
denotes |
of |
T7724 |
30368-30380 |
NN |
denotes |
vesiculation |
T7725 |
30381-30387 |
NN |
denotes |
buffer |
T7726 |
30388-30389 |
-LRB- |
denotes |
( |
T7727 |
30428-30433 |
NN |
denotes |
CaCl2 |
T7728 |
30389-30391 |
CD |
denotes |
20 |
T7729 |
30392-30394 |
NN |
denotes |
mM |
T7730 |
30395-30400 |
NN |
denotes |
Hepes |
T7731 |
30400-30402 |
, |
denotes |
, |
T7732 |
30402-30404 |
NN |
denotes |
pH |
T7733 |
30405-30408 |
CD |
denotes |
7.5 |
T7734 |
30408-30410 |
, |
denotes |
, |
T7735 |
30410-30413 |
CD |
denotes |
150 |
T7736 |
30414-30416 |
NN |
denotes |
mM |
T7737 |
30417-30421 |
NN |
denotes |
NaCl |
T7738 |
30421-30423 |
, |
denotes |
, |
T7739 |
30423-30424 |
CD |
denotes |
1 |
T7740 |
30425-30427 |
NN |
denotes |
mM |
T7741 |
30434-30442 |
VBN |
denotes |
adjusted |
T7742 |
30443-30445 |
IN |
denotes |
to |
T7743 |
30446-30447 |
CD |
denotes |
5 |
T7744 |
30448-30450 |
NN |
denotes |
μM |
T7745 |
30466-30472 |
NN |
denotes |
A23187 |
T7746 |
30451-30455 |
NN |
denotes |
Ca2+ |
T7747 |
30456-30465 |
NN |
denotes |
ionophore |
T7748 |
30472-30473 |
-RRB- |
denotes |
) |
T7749 |
30474-30477 |
CC |
denotes |
and |
T7750 |
30478-30487 |
VBN |
denotes |
incubated |
T7751 |
30488-30491 |
IN |
denotes |
for |
T7752 |
30492-30494 |
CD |
denotes |
30 |
T7753 |
30495-30502 |
NNS |
denotes |
minutes |
T7754 |
30503-30505 |
IN |
denotes |
at |
T7755 |
30506-30508 |
CD |
denotes |
37 |
T7756 |
30508-30510 |
NNS |
denotes |
°C |
T7757 |
30510-30511 |
. |
denotes |
. |
T7758 |
30511-30642 |
sentence |
denotes |
After incubation EDTA was added to the solution to a final concentration of 5 mM and centrifuged for 5 minutes at 400 × g and 4°C. |
T7759 |
30512-30517 |
IN |
denotes |
After |
T7760 |
30538-30543 |
VBN |
denotes |
added |
T7761 |
30518-30528 |
NN |
denotes |
incubation |
T7762 |
30529-30533 |
NN |
denotes |
EDTA |
T7763 |
30534-30537 |
VBD |
denotes |
was |
T7764 |
30544-30546 |
IN |
denotes |
to |
T7765 |
30547-30550 |
DT |
denotes |
the |
T7766 |
30551-30559 |
NN |
denotes |
solution |
T7767 |
30560-30562 |
IN |
denotes |
to |
T7768 |
30563-30564 |
DT |
denotes |
a |
T7769 |
30571-30584 |
NN |
denotes |
concentration |
T7770 |
30565-30570 |
JJ |
denotes |
final |
T7771 |
30585-30587 |
IN |
denotes |
of |
T7772 |
30588-30589 |
CD |
denotes |
5 |
T7773 |
30590-30592 |
NN |
denotes |
mM |
T7774 |
30593-30596 |
CC |
denotes |
and |
T7775 |
30597-30608 |
VBN |
denotes |
centrifuged |
T7776 |
30609-30612 |
IN |
denotes |
for |
T7777 |
30613-30614 |
CD |
denotes |
5 |
T7778 |
30615-30622 |
NNS |
denotes |
minutes |
T7779 |
30623-30625 |
IN |
denotes |
at |
T7780 |
30626-30629 |
CD |
denotes |
400 |
T7781 |
30632-30633 |
NN |
denotes |
g |
T7782 |
30630-30631 |
SYM |
denotes |
× |
T7783 |
30634-30637 |
CC |
denotes |
and |
T7784 |
30638-30639 |
CD |
denotes |
4 |
T7785 |
30639-30641 |
NNS |
denotes |
°C |
T7786 |
30641-30642 |
. |
denotes |
. |
T7787 |
30642-30726 |
sentence |
denotes |
The supernatant was collected and centrifuged for 20 minutes at 15,000 × g and 4°C. |
T7788 |
30643-30646 |
DT |
denotes |
The |
T7789 |
30647-30658 |
NN |
denotes |
supernatant |
T7790 |
30663-30672 |
VBN |
denotes |
collected |
T7791 |
30659-30662 |
VBD |
denotes |
was |
T7792 |
30673-30676 |
CC |
denotes |
and |
T7793 |
30677-30688 |
VBN |
denotes |
centrifuged |
T7794 |
30689-30692 |
IN |
denotes |
for |
T7795 |
30693-30695 |
CD |
denotes |
20 |
T7796 |
30696-30703 |
NNS |
denotes |
minutes |
T7797 |
30704-30706 |
IN |
denotes |
at |
T7798 |
30707-30713 |
CD |
denotes |
15,000 |
T7799 |
30716-30717 |
NN |
denotes |
g |
T7800 |
30714-30715 |
SYM |
denotes |
× |
T7801 |
30718-30721 |
CC |
denotes |
and |
T7802 |
30722-30723 |
CD |
denotes |
4 |
T7803 |
30723-30725 |
NNS |
denotes |
°C |
T7804 |
30725-30726 |
. |
denotes |
. |
T7805 |
30726-30883 |
sentence |
denotes |
The resulting pellet was resuspended in washing buffer and centrifuged at 400 × g again to collect a supernatant enriched with red blood cell microvesicles. |
T7806 |
30727-30730 |
DT |
denotes |
The |
T7807 |
30741-30747 |
NN |
denotes |
pellet |
T7808 |
30731-30740 |
VBG |
denotes |
resulting |
T7809 |
30752-30763 |
VBN |
denotes |
resuspended |
T7810 |
30748-30751 |
VBD |
denotes |
was |
T7811 |
30764-30766 |
IN |
denotes |
in |
T7812 |
30767-30774 |
VBG |
denotes |
washing |
T7813 |
30775-30781 |
NN |
denotes |
buffer |
T7814 |
30782-30785 |
CC |
denotes |
and |
T7815 |
30786-30797 |
VBN |
denotes |
centrifuged |
T7816 |
30798-30800 |
IN |
denotes |
at |
T7817 |
30801-30804 |
CD |
denotes |
400 |
T7818 |
30807-30808 |
NN |
denotes |
g |
T7819 |
30805-30806 |
SYM |
denotes |
× |
T7820 |
30809-30814 |
RB |
denotes |
again |
T7821 |
30815-30817 |
TO |
denotes |
to |
T7822 |
30818-30825 |
VB |
denotes |
collect |
T7823 |
30826-30827 |
DT |
denotes |
a |
T7824 |
30828-30839 |
NN |
denotes |
supernatant |
T7825 |
30840-30848 |
VBN |
denotes |
enriched |
T7826 |
30849-30853 |
IN |
denotes |
with |
T7827 |
30854-30857 |
JJ |
denotes |
red |
T7828 |
30864-30868 |
NN |
denotes |
cell |
T7829 |
30858-30863 |
NN |
denotes |
blood |
T7830 |
30869-30882 |
NNS |
denotes |
microvesicles |
T7831 |
30882-30883 |
. |
denotes |
. |
T7832 |
30883-30985 |
sentence |
denotes |
The supernatant of the 15,000 × g step was further centrifuged for 60 minutes at 100,000 × g and 4°C. |
T7833 |
30884-30887 |
DT |
denotes |
The |
T7834 |
30888-30899 |
NN |
denotes |
supernatant |
T7835 |
30935-30946 |
VBN |
denotes |
centrifuged |
T7836 |
30900-30902 |
IN |
denotes |
of |
T7837 |
30903-30906 |
DT |
denotes |
the |
T7838 |
30918-30922 |
NN |
denotes |
step |
T7839 |
30907-30913 |
CD |
denotes |
15,000 |
T7840 |
30916-30917 |
NN |
denotes |
g |
T7841 |
30914-30915 |
SYM |
denotes |
× |
T7842 |
30923-30926 |
VBD |
denotes |
was |
T7843 |
30927-30934 |
RBR |
denotes |
further |
T7844 |
30947-30950 |
IN |
denotes |
for |
T7845 |
30951-30953 |
CD |
denotes |
60 |
T7846 |
30954-30961 |
NNS |
denotes |
minutes |
T7847 |
30962-30964 |
IN |
denotes |
at |
T7848 |
30965-30972 |
CD |
denotes |
100,000 |
T7849 |
30975-30976 |
NN |
denotes |
g |
T7850 |
30973-30974 |
SYM |
denotes |
× |
T7851 |
30977-30980 |
CC |
denotes |
and |
T7852 |
30981-30982 |
CD |
denotes |
4 |
T7853 |
30982-30984 |
NNS |
denotes |
°C |
T7854 |
30984-30985 |
. |
denotes |
. |
T7855 |
30985-31155 |
sentence |
denotes |
The final pellet of this step was resuspended in washing buffer and centrifuged at 15,000 × g again to purify a supernatant enriched in nanovesicles according to [7,20]. |
T7856 |
30986-30989 |
DT |
denotes |
The |
T7857 |
30996-31002 |
NN |
denotes |
pellet |
T7858 |
30990-30995 |
JJ |
denotes |
final |
T7859 |
31020-31031 |
VBN |
denotes |
resuspended |
T7860 |
31003-31005 |
IN |
denotes |
of |
T7861 |
31006-31010 |
DT |
denotes |
this |
T7862 |
31011-31015 |
NN |
denotes |
step |
T7863 |
31016-31019 |
VBD |
denotes |
was |
T7864 |
31032-31034 |
IN |
denotes |
in |
T7865 |
31035-31042 |
VBG |
denotes |
washing |
T7866 |
31043-31049 |
NN |
denotes |
buffer |
T7867 |
31050-31053 |
CC |
denotes |
and |
T7868 |
31054-31065 |
VBN |
denotes |
centrifuged |
T7869 |
31066-31068 |
IN |
denotes |
at |
T7870 |
31069-31075 |
CD |
denotes |
15,000 |
T7871 |
31078-31079 |
NN |
denotes |
g |
T7872 |
31076-31077 |
SYM |
denotes |
× |
T7873 |
31080-31085 |
RB |
denotes |
again |
T7874 |
31086-31088 |
TO |
denotes |
to |
T7875 |
31089-31095 |
VB |
denotes |
purify |
T7876 |
31096-31097 |
DT |
denotes |
a |
T7877 |
31098-31109 |
NN |
denotes |
supernatant |
T7878 |
31110-31118 |
VBN |
denotes |
enriched |
T7879 |
31119-31121 |
IN |
denotes |
in |
T7880 |
31122-31134 |
NNS |
denotes |
nanovesicles |
T7881 |
31135-31144 |
VBG |
denotes |
according |
T7882 |
31145-31147 |
IN |
denotes |
to |
T7883 |
31148-31149 |
-LRB- |
denotes |
[ |
T7884 |
31149-31150 |
CD |
denotes |
7 |
T7885 |
31151-31153 |
CD |
denotes |
20 |
T7886 |
31150-31151 |
, |
denotes |
, |
T7887 |
31153-31154 |
-RRB- |
denotes |
] |
T7888 |
31154-31155 |
. |
denotes |
. |
T7889 |
31155-31248 |
sentence |
denotes |
The vesicles were assayed for acetylcholine esterase activity and used for further analysis. |
T7890 |
31156-31159 |
DT |
denotes |
The |
T7891 |
31160-31168 |
NNS |
denotes |
vesicles |
T7892 |
31174-31181 |
VBN |
denotes |
assayed |
T7893 |
31169-31173 |
VBD |
denotes |
were |
T7894 |
31182-31185 |
IN |
denotes |
for |
T7895 |
31186-31199 |
NN |
denotes |
acetylcholine |
T7896 |
31200-31208 |
NN |
denotes |
esterase |
T7897 |
31209-31217 |
NN |
denotes |
activity |
T7898 |
31218-31221 |
CC |
denotes |
and |
T7899 |
31222-31226 |
VBN |
denotes |
used |
T7900 |
31227-31230 |
IN |
denotes |
for |
T7901 |
31231-31238 |
JJ |
denotes |
further |
T7902 |
31239-31247 |
NN |
denotes |
analysis |
T7903 |
31247-31248 |
. |
denotes |
. |
T7951 |
31250-31263 |
NN |
denotes |
Acetylcholine |
T7952 |
31264-31272 |
NN |
denotes |
esterase |
T7953 |
31273-31278 |
NN |
denotes |
assay |
T7954 |
31278-31463 |
sentence |
denotes |
A 30 μl sample of red blood cell vesicles was mixed with an equal volume of buffer (20 mM Hepes, pH 7.6, 150 mM NaCl, 0.5 % Triton X-100), vortexed and incubated for 5 minutes at 37°C. |
T7955 |
31279-31280 |
DT |
denotes |
A |
T7956 |
31287-31293 |
NN |
denotes |
sample |
T7957 |
31281-31283 |
CD |
denotes |
30 |
T7958 |
31284-31286 |
NN |
denotes |
μl |
T7959 |
31325-31330 |
VBN |
denotes |
mixed |
T7960 |
31294-31296 |
IN |
denotes |
of |
T7961 |
31297-31300 |
JJ |
denotes |
red |
T7962 |
31307-31311 |
NN |
denotes |
cell |
T7963 |
31301-31306 |
NN |
denotes |
blood |
T7964 |
31312-31320 |
NNS |
denotes |
vesicles |
T7965 |
31321-31324 |
VBD |
denotes |
was |
T7966 |
31331-31335 |
IN |
denotes |
with |
T7967 |
31336-31338 |
DT |
denotes |
an |
T7968 |
31345-31351 |
NN |
denotes |
volume |
T7969 |
31339-31344 |
JJ |
denotes |
equal |
T7970 |
31352-31354 |
IN |
denotes |
of |
T7971 |
31355-31361 |
NN |
denotes |
buffer |
T7972 |
31362-31363 |
-LRB- |
denotes |
( |
T7973 |
31410-31411 |
NN |
denotes |
X |
T7974 |
31363-31365 |
CD |
denotes |
20 |
T7975 |
31366-31368 |
NN |
denotes |
mM |
T7976 |
31369-31374 |
NN |
denotes |
Hepes |
T7977 |
31374-31376 |
, |
denotes |
, |
T7978 |
31376-31378 |
NN |
denotes |
pH |
T7979 |
31379-31382 |
CD |
denotes |
7.6 |
T7980 |
31382-31384 |
, |
denotes |
, |
T7981 |
31384-31387 |
CD |
denotes |
150 |
T7982 |
31388-31390 |
NN |
denotes |
mM |
T7983 |
31391-31395 |
NN |
denotes |
NaCl |
T7984 |
31395-31397 |
, |
denotes |
, |
T7985 |
31397-31400 |
CD |
denotes |
0.5 |
T7986 |
31401-31402 |
NN |
denotes |
% |
T7987 |
31403-31409 |
NN |
denotes |
Triton |
T7988 |
31411-31412 |
HYPH |
denotes |
- |
T7989 |
31412-31415 |
CD |
denotes |
100 |
T7990 |
31415-31416 |
-RRB- |
denotes |
) |
T7991 |
31416-31418 |
, |
denotes |
, |
T7992 |
31418-31426 |
VBN |
denotes |
vortexed |
T7993 |
31427-31430 |
CC |
denotes |
and |
T7994 |
31431-31440 |
VBN |
denotes |
incubated |
T7995 |
31441-31444 |
IN |
denotes |
for |
T7996 |
31445-31446 |
CD |
denotes |
5 |
T7997 |
31447-31454 |
NNS |
denotes |
minutes |
T7998 |
31455-31457 |
IN |
denotes |
at |
T7999 |
31458-31460 |
CD |
denotes |
37 |
T8000 |
31460-31462 |
NNS |
denotes |
°C |
T8001 |
31462-31463 |
. |
denotes |
. |
T8002 |
31463-31683 |
sentence |
denotes |
Subsequently 640 μl sodium phosphate buffer (100 mM Na2PO4, pH 7.6), 50 μl DTNB (5,5'-dithiobis-(2-nitrobenzoic acid), 10 mM in sodium phosphate buffer), and 50 μl acetylthiocholine chloride (12,5 mM in H2O) were added. |
T8003 |
31464-31476 |
RB |
denotes |
Subsequently |
T8004 |
31677-31682 |
VBN |
denotes |
added |
T8005 |
31477-31480 |
CD |
denotes |
640 |
T8006 |
31481-31483 |
NN |
denotes |
μl |
T8007 |
31501-31507 |
NN |
denotes |
buffer |
T8008 |
31484-31490 |
NN |
denotes |
sodium |
T8009 |
31491-31500 |
NN |
denotes |
phosphate |
T8010 |
31508-31509 |
-LRB- |
denotes |
( |
T8011 |
31516-31522 |
NN |
denotes |
Na2PO4 |
T8012 |
31509-31512 |
CD |
denotes |
100 |
T8013 |
31513-31515 |
NN |
denotes |
mM |
T8014 |
31522-31524 |
, |
denotes |
, |
T8015 |
31524-31526 |
NN |
denotes |
pH |
T8016 |
31527-31530 |
CD |
denotes |
7.6 |
T8017 |
31530-31531 |
-RRB- |
denotes |
) |
T8018 |
31531-31533 |
, |
denotes |
, |
T8019 |
31533-31535 |
CD |
denotes |
50 |
T8020 |
31536-31538 |
NN |
denotes |
μl |
T8021 |
31539-31543 |
NN |
denotes |
DTNB |
T8022 |
31544-31545 |
-LRB- |
denotes |
( |
T8023 |
31586-31588 |
NN |
denotes |
mM |
T8024 |
31545-31546 |
CD |
denotes |
5 |
T8025 |
31550-31559 |
NN |
denotes |
dithiobis |
T8026 |
31546-31547 |
, |
denotes |
, |
T8027 |
31547-31548 |
CD |
denotes |
5 |
T8028 |
31548-31549 |
SYM |
denotes |
' |
T8029 |
31549-31550 |
HYPH |
denotes |
- |
T8030 |
31559-31560 |
HYPH |
denotes |
- |
T8031 |
31560-31561 |
-LRB- |
denotes |
( |
T8032 |
31576-31580 |
NN |
denotes |
acid |
T8033 |
31561-31562 |
CD |
denotes |
2 |
T8034 |
31562-31563 |
HYPH |
denotes |
- |
T8035 |
31563-31575 |
JJ |
denotes |
nitrobenzoic |
T8036 |
31580-31581 |
-RRB- |
denotes |
) |
T8037 |
31581-31583 |
, |
denotes |
, |
T8038 |
31583-31585 |
CD |
denotes |
10 |
T8039 |
31589-31591 |
IN |
denotes |
in |
T8040 |
31592-31598 |
NN |
denotes |
sodium |
T8041 |
31599-31608 |
NN |
denotes |
phosphate |
T8042 |
31609-31615 |
NN |
denotes |
buffer |
T8043 |
31615-31616 |
-RRB- |
denotes |
) |
T8044 |
31616-31618 |
, |
denotes |
, |
T8045 |
31618-31621 |
CC |
denotes |
and |
T8046 |
31622-31624 |
CD |
denotes |
50 |
T8047 |
31625-31627 |
NN |
denotes |
μl |
T8048 |
31646-31654 |
NN |
denotes |
chloride |
T8049 |
31628-31645 |
NN |
denotes |
acetylthiocholine |
T8050 |
31655-31656 |
-LRB- |
denotes |
( |
T8051 |
31661-31663 |
NN |
denotes |
mM |
T8052 |
31656-31658 |
CD |
denotes |
12 |
T8053 |
31658-31659 |
, |
denotes |
, |
T8054 |
31659-31660 |
CD |
denotes |
5 |
T8055 |
31664-31666 |
IN |
denotes |
in |
T8056 |
31667-31670 |
NN |
denotes |
H2O |
T8057 |
31670-31671 |
-RRB- |
denotes |
) |
T8058 |
31672-31676 |
VBD |
denotes |
were |
T8059 |
31682-31683 |
. |
denotes |
. |
T8060 |
31683-31825 |
sentence |
denotes |
The reaction took place at room temperature and was measured photometrically at a wavelength of 405 nm according to the Ellmann' method [25]. |
T8061 |
31684-31687 |
DT |
denotes |
The |
T8062 |
31688-31696 |
NN |
denotes |
reaction |
T8063 |
31697-31701 |
VBD |
denotes |
took |
T8064 |
31702-31707 |
NN |
denotes |
place |
T8065 |
31708-31710 |
IN |
denotes |
at |
T8066 |
31711-31715 |
NN |
denotes |
room |
T8067 |
31716-31727 |
NN |
denotes |
temperature |
T8068 |
31728-31731 |
CC |
denotes |
and |
T8069 |
31732-31735 |
VBD |
denotes |
was |
T8070 |
31736-31744 |
VBN |
denotes |
measured |
T8071 |
31745-31760 |
RB |
denotes |
photometrically |
T8072 |
31761-31763 |
IN |
denotes |
at |
T8073 |
31764-31765 |
DT |
denotes |
a |
T8074 |
31766-31776 |
NN |
denotes |
wavelength |
T8075 |
31777-31779 |
IN |
denotes |
of |
T8076 |
31780-31783 |
CD |
denotes |
405 |
T8077 |
31784-31786 |
NNS |
denotes |
nm |
T8078 |
31787-31796 |
VBG |
denotes |
according |
T8079 |
31797-31799 |
IN |
denotes |
to |
T8080 |
31800-31803 |
DT |
denotes |
the |
T8081 |
31813-31819 |
NN |
denotes |
method |
T8082 |
31804-31811 |
NNP |
denotes |
Ellmann |
T8083 |
31811-31812 |
POS |
denotes |
' |
T8084 |
31820-31821 |
-LRB- |
denotes |
[ |
T8085 |
31821-31823 |
CD |
denotes |
25 |
T8086 |
31823-31824 |
-RRB- |
denotes |
] |
T8087 |
31824-31825 |
. |
denotes |
. |
T8113 |
31827-31840 |
NN |
denotes |
Determination |
T8114 |
31841-31843 |
IN |
denotes |
of |
T8115 |
31844-31847 |
DT |
denotes |
the |
T8116 |
31856-31866 |
NN |
denotes |
resistance |
T8117 |
31848-31855 |
JJ |
denotes |
osmotic |
T8118 |
31867-31869 |
IN |
denotes |
of |
T8119 |
31870-31873 |
JJ |
denotes |
red |
T8120 |
31880-31885 |
NNS |
denotes |
cells |
T8121 |
31874-31879 |
NN |
denotes |
blood |
T8122 |
31885-32068 |
sentence |
denotes |
A Na2HPO4/NaH2PO4 buffered NaCl gradient (pH 7.4) comprised of the following NaCl steps was used for this assay: 0.90%, 0.65%, 0.60%, 0.55%, 0.50%, 0.45%, 0.40%, 0.35%, 0.30%, 0.10%. |
T8123 |
31886-31887 |
DT |
denotes |
A |
T8124 |
31918-31926 |
NN |
denotes |
gradient |
T8125 |
31888-31895 |
NN |
denotes |
Na2HPO4 |
T8126 |
31896-31903 |
NN |
denotes |
NaH2PO4 |
T8127 |
31895-31896 |
HYPH |
denotes |
/ |
T8128 |
31904-31912 |
VBN |
denotes |
buffered |
T8129 |
31913-31917 |
NN |
denotes |
NaCl |
T8130 |
31978-31982 |
VBN |
denotes |
used |
T8131 |
31927-31928 |
-LRB- |
denotes |
( |
T8132 |
31928-31930 |
NN |
denotes |
pH |
T8133 |
31931-31934 |
CD |
denotes |
7.4 |
T8134 |
31934-31935 |
-RRB- |
denotes |
) |
T8135 |
31936-31945 |
VBN |
denotes |
comprised |
T8136 |
31946-31948 |
IN |
denotes |
of |
T8137 |
31949-31952 |
DT |
denotes |
the |
T8138 |
31968-31973 |
NNS |
denotes |
steps |
T8139 |
31953-31962 |
VBG |
denotes |
following |
T8140 |
31963-31967 |
NN |
denotes |
NaCl |
T8141 |
31974-31977 |
VBD |
denotes |
was |
T8142 |
31983-31986 |
IN |
denotes |
for |
T8143 |
31987-31991 |
DT |
denotes |
this |
T8144 |
31992-31997 |
NN |
denotes |
assay |
T8145 |
31997-31999 |
: |
denotes |
: |
T8146 |
31999-32003 |
CD |
denotes |
0.90 |
T8147 |
32003-32004 |
NN |
denotes |
% |
T8148 |
32004-32006 |
, |
denotes |
, |
T8149 |
32006-32010 |
CD |
denotes |
0.65 |
T8150 |
32010-32011 |
NN |
denotes |
% |
T8151 |
32011-32013 |
, |
denotes |
, |
T8152 |
32013-32017 |
CD |
denotes |
0.60 |
T8153 |
32017-32018 |
NN |
denotes |
% |
T8154 |
32018-32020 |
, |
denotes |
, |
T8155 |
32020-32024 |
CD |
denotes |
0.55 |
T8156 |
32024-32025 |
NN |
denotes |
% |
T8157 |
32025-32027 |
, |
denotes |
, |
T8158 |
32027-32031 |
CD |
denotes |
0.50 |
T8159 |
32031-32032 |
NN |
denotes |
% |
T8160 |
32032-32034 |
, |
denotes |
, |
T8161 |
32034-32038 |
CD |
denotes |
0.45 |
T8162 |
32038-32039 |
NN |
denotes |
% |
T8163 |
32039-32041 |
, |
denotes |
, |
T8164 |
32041-32045 |
CD |
denotes |
0.40 |
T8165 |
32045-32046 |
NN |
denotes |
% |
T8166 |
32046-32048 |
, |
denotes |
, |
T8167 |
32048-32052 |
CD |
denotes |
0.35 |
T8168 |
32052-32053 |
NN |
denotes |
% |
T8169 |
32053-32055 |
, |
denotes |
, |
T8170 |
32055-32059 |
CD |
denotes |
0.30 |
T8171 |
32059-32060 |
NN |
denotes |
% |
T8172 |
32060-32062 |
, |
denotes |
, |
T8173 |
32062-32066 |
CD |
denotes |
0.10 |
T8174 |
32066-32067 |
NN |
denotes |
% |
T8175 |
32067-32068 |
. |
denotes |
. |
T8176 |
32068-32217 |
sentence |
denotes |
For each step 5 ml of the gradient step solution and 50 μl blood sample were carefully inverted four times and incubated for 30 minutes in the dark. |
T8177 |
32069-32072 |
IN |
denotes |
For |
T8178 |
32156-32164 |
VBN |
denotes |
inverted |
T8179 |
32073-32077 |
DT |
denotes |
each |
T8180 |
32078-32082 |
NN |
denotes |
step |
T8181 |
32083-32084 |
CD |
denotes |
5 |
T8182 |
32085-32087 |
NNS |
denotes |
ml |
T8183 |
32088-32090 |
IN |
denotes |
of |
T8184 |
32091-32094 |
DT |
denotes |
the |
T8185 |
32109-32117 |
NN |
denotes |
solution |
T8186 |
32095-32103 |
NN |
denotes |
gradient |
T8187 |
32104-32108 |
NN |
denotes |
step |
T8188 |
32118-32121 |
CC |
denotes |
and |
T8189 |
32122-32124 |
CD |
denotes |
50 |
T8190 |
32125-32127 |
NN |
denotes |
μl |
T8191 |
32134-32140 |
NN |
denotes |
sample |
T8192 |
32128-32133 |
NN |
denotes |
blood |
T8193 |
32141-32145 |
VBD |
denotes |
were |
T8194 |
32146-32155 |
RB |
denotes |
carefully |
T8195 |
32165-32169 |
CD |
denotes |
four |
T8196 |
32170-32175 |
NNS |
denotes |
times |
T8197 |
32176-32179 |
CC |
denotes |
and |
T8198 |
32180-32189 |
VBN |
denotes |
incubated |
T8199 |
32190-32193 |
IN |
denotes |
for |
T8200 |
32194-32196 |
CD |
denotes |
30 |
T8201 |
32197-32204 |
NNS |
denotes |
minutes |
T8202 |
32205-32207 |
IN |
denotes |
in |
T8203 |
32208-32211 |
DT |
denotes |
the |
T8204 |
32212-32216 |
NN |
denotes |
dark |
T8205 |
32216-32217 |
. |
denotes |
. |
T8206 |
32217-32300 |
sentence |
denotes |
Samples were carefully inverted again and centrifuged at 1,500 × g for 10 minutes. |
T8207 |
32218-32225 |
NNS |
denotes |
Samples |
T8208 |
32241-32249 |
VBN |
denotes |
inverted |
T8209 |
32226-32230 |
VBD |
denotes |
were |
T8210 |
32231-32240 |
RB |
denotes |
carefully |
T8211 |
32250-32255 |
RB |
denotes |
again |
T8212 |
32256-32259 |
CC |
denotes |
and |
T8213 |
32260-32271 |
VBN |
denotes |
centrifuged |
T8214 |
32272-32274 |
IN |
denotes |
at |
T8215 |
32275-32280 |
CD |
denotes |
1,500 |
T8216 |
32283-32284 |
NN |
denotes |
g |
T8217 |
32281-32282 |
SYM |
denotes |
× |
T8218 |
32285-32288 |
IN |
denotes |
for |
T8219 |
32289-32291 |
CD |
denotes |
10 |
T8220 |
32292-32299 |
NNS |
denotes |
minutes |
T8221 |
32299-32300 |
. |
denotes |
. |
T8222 |
32300-32385 |
sentence |
denotes |
From 1 ml supernatant of each step the extinction at 546 nm wavelength was measured. |
T8223 |
32301-32305 |
IN |
denotes |
From |
T8224 |
32376-32384 |
VBN |
denotes |
measured |
T8225 |
32306-32307 |
CD |
denotes |
1 |
T8226 |
32308-32310 |
NN |
denotes |
ml |
T8227 |
32311-32322 |
NN |
denotes |
supernatant |
T8228 |
32323-32325 |
IN |
denotes |
of |
T8229 |
32326-32330 |
DT |
denotes |
each |
T8230 |
32331-32335 |
NN |
denotes |
step |
T8231 |
32336-32339 |
DT |
denotes |
the |
T8232 |
32340-32350 |
NN |
denotes |
extinction |
T8233 |
32351-32353 |
IN |
denotes |
at |
T8234 |
32354-32357 |
CD |
denotes |
546 |
T8235 |
32358-32360 |
NN |
denotes |
nm |
T8236 |
32361-32371 |
NN |
denotes |
wavelength |
T8237 |
32372-32375 |
VBD |
denotes |
was |
T8238 |
32384-32385 |
. |
denotes |
. |
T8239 |
32385-32435 |
sentence |
denotes |
The experiment was performed at room temperature. |
T8240 |
32386-32389 |
DT |
denotes |
The |
T8241 |
32390-32400 |
NN |
denotes |
experiment |
T8242 |
32405-32414 |
VBN |
denotes |
performed |
T8243 |
32401-32404 |
VBD |
denotes |
was |
T8244 |
32415-32417 |
IN |
denotes |
at |
T8245 |
32418-32422 |
NN |
denotes |
room |
T8246 |
32423-32434 |
NN |
denotes |
temperature |
T8247 |
32434-32435 |
. |
denotes |
. |
T8326 |
32437-32445 |
NN |
denotes |
Platelet |
T8327 |
32446-32457 |
NN |
denotes |
aggregation |
T8328 |
32458-32463 |
NN |
denotes |
assay |
T8329 |
32463-32544 |
sentence |
denotes |
Approximately 500 μl of murine blood were centrifuged at 200 × g for 15 minutes. |
T8330 |
32464-32477 |
RB |
denotes |
Approximately |
T8331 |
32478-32481 |
CD |
denotes |
500 |
T8332 |
32482-32484 |
NNS |
denotes |
μl |
T8333 |
32506-32517 |
VBN |
denotes |
centrifuged |
T8334 |
32485-32487 |
IN |
denotes |
of |
T8335 |
32488-32494 |
JJ |
denotes |
murine |
T8336 |
32495-32500 |
NN |
denotes |
blood |
T8337 |
32501-32505 |
VBD |
denotes |
were |
T8338 |
32518-32520 |
IN |
denotes |
at |
T8339 |
32521-32524 |
CD |
denotes |
200 |
T8340 |
32527-32528 |
NN |
denotes |
g |
T8341 |
32525-32526 |
SYM |
denotes |
× |
T8342 |
32529-32532 |
IN |
denotes |
for |
T8343 |
32533-32535 |
CD |
denotes |
15 |
T8344 |
32536-32543 |
NNS |
denotes |
minutes |
T8345 |
32543-32544 |
. |
denotes |
. |
T8346 |
32544-32641 |
sentence |
denotes |
The resulting supernatants were transferred to new tubes and used as platelet rich plasma (PRP). |
T8347 |
32545-32548 |
DT |
denotes |
The |
T8348 |
32559-32571 |
NNS |
denotes |
supernatants |
T8349 |
32549-32558 |
VBG |
denotes |
resulting |
T8350 |
32577-32588 |
VBN |
denotes |
transferred |
T8351 |
32572-32576 |
VBD |
denotes |
were |
T8352 |
32589-32591 |
IN |
denotes |
to |
T8353 |
32592-32595 |
JJ |
denotes |
new |
T8354 |
32596-32601 |
NNS |
denotes |
tubes |
T8355 |
32602-32605 |
CC |
denotes |
and |
T8356 |
32606-32610 |
VBN |
denotes |
used |
T8357 |
32611-32613 |
IN |
denotes |
as |
T8358 |
32614-32622 |
NN |
denotes |
platelet |
T8359 |
32623-32627 |
JJ |
denotes |
rich |
T8360 |
32628-32634 |
NN |
denotes |
plasma |
T8361 |
32635-32636 |
-LRB- |
denotes |
( |
T8362 |
32636-32639 |
NN |
denotes |
PRP |
T8363 |
32639-32640 |
-RRB- |
denotes |
) |
T8364 |
32640-32641 |
. |
denotes |
. |
T8365 |
32641-32834 |
sentence |
denotes |
The platelet aggregation measurements were done according to the method developed by Born and Cross [26] using an APACT photometer (Labor, Ahrendsburg, FRG) and the APACT software (APACT 1.4). |
T8366 |
32642-32645 |
DT |
denotes |
The |
T8367 |
32667-32679 |
NNS |
denotes |
measurements |
T8368 |
32646-32654 |
NN |
denotes |
platelet |
T8369 |
32655-32666 |
NN |
denotes |
aggregation |
T8370 |
32685-32689 |
VBN |
denotes |
done |
T8371 |
32680-32684 |
VBD |
denotes |
were |
T8372 |
32690-32699 |
VBG |
denotes |
according |
T8373 |
32700-32702 |
IN |
denotes |
to |
T8374 |
32703-32706 |
DT |
denotes |
the |
T8375 |
32707-32713 |
NN |
denotes |
method |
T8376 |
32714-32723 |
VBN |
denotes |
developed |
T8377 |
32724-32726 |
IN |
denotes |
by |
T8378 |
32727-32731 |
NNP |
denotes |
Born |
T8379 |
32732-32735 |
CC |
denotes |
and |
T8380 |
32736-32741 |
NNP |
denotes |
Cross |
T8381 |
32742-32743 |
-LRB- |
denotes |
[ |
T8382 |
32743-32745 |
CD |
denotes |
26 |
T8383 |
32745-32746 |
-RRB- |
denotes |
] |
T8384 |
32747-32752 |
VBG |
denotes |
using |
T8385 |
32753-32755 |
DT |
denotes |
an |
T8386 |
32762-32772 |
NN |
denotes |
photometer |
T8387 |
32756-32761 |
NN |
denotes |
APACT |
T8388 |
32773-32774 |
-LRB- |
denotes |
( |
T8389 |
32794-32797 |
NN |
denotes |
FRG |
T8390 |
32774-32779 |
NN |
denotes |
Labor |
T8391 |
32779-32781 |
, |
denotes |
, |
T8392 |
32781-32792 |
NN |
denotes |
Ahrendsburg |
T8393 |
32792-32794 |
, |
denotes |
, |
T8394 |
32797-32798 |
-RRB- |
denotes |
) |
T8395 |
32799-32802 |
CC |
denotes |
and |
T8396 |
32803-32806 |
DT |
denotes |
the |
T8397 |
32813-32821 |
NN |
denotes |
software |
T8398 |
32807-32812 |
NN |
denotes |
APACT |
T8399 |
32822-32823 |
-LRB- |
denotes |
( |
T8400 |
32823-32828 |
NN |
denotes |
APACT |
T8401 |
32829-32832 |
CD |
denotes |
1.4 |
T8402 |
32832-32833 |
-RRB- |
denotes |
) |
T8403 |
32833-32834 |
. |
denotes |
. |
T8404 |
32834-32951 |
sentence |
denotes |
Subsequently samples of the PRP were adjusted with buffer (5 mM Hepes, 150 mM NaCl, pH 7.3) to 250,000 platelets/μl. |
T8405 |
32835-32847 |
RB |
denotes |
Subsequently |
T8406 |
32872-32880 |
VBN |
denotes |
adjusted |
T8407 |
32848-32855 |
NNS |
denotes |
samples |
T8408 |
32856-32858 |
IN |
denotes |
of |
T8409 |
32859-32862 |
DT |
denotes |
the |
T8410 |
32863-32866 |
NN |
denotes |
PRP |
T8411 |
32867-32871 |
VBD |
denotes |
were |
T8412 |
32881-32885 |
IN |
denotes |
with |
T8413 |
32886-32892 |
NN |
denotes |
buffer |
T8414 |
32893-32894 |
-LRB- |
denotes |
( |
T8415 |
32919-32921 |
NN |
denotes |
pH |
T8416 |
32894-32895 |
CD |
denotes |
5 |
T8417 |
32896-32898 |
NN |
denotes |
mM |
T8418 |
32899-32904 |
NN |
denotes |
Hepes |
T8419 |
32904-32906 |
, |
denotes |
, |
T8420 |
32906-32909 |
CD |
denotes |
150 |
T8421 |
32910-32912 |
NN |
denotes |
mM |
T8422 |
32913-32917 |
NN |
denotes |
NaCl |
T8423 |
32917-32919 |
, |
denotes |
, |
T8424 |
32922-32925 |
CD |
denotes |
7.3 |
T8425 |
32925-32926 |
-RRB- |
denotes |
) |
T8426 |
32927-32929 |
IN |
denotes |
to |
T8427 |
32930-32937 |
CD |
denotes |
250,000 |
T8428 |
32938-32947 |
NNS |
denotes |
platelets |
T8429 |
32947-32948 |
SYM |
denotes |
/ |
T8430 |
32948-32950 |
NN |
denotes |
μl |
T8431 |
32950-32951 |
. |
denotes |
. |
T8432 |
32951-33076 |
sentence |
denotes |
A constant platelet count was used throughout allowing comparisons between wild type and knock out murine and human samples. |
T8433 |
32952-32953 |
DT |
denotes |
A |
T8434 |
32972-32977 |
NN |
denotes |
count |
T8435 |
32954-32962 |
JJ |
denotes |
constant |
T8436 |
32963-32971 |
NN |
denotes |
platelet |
T8437 |
32982-32986 |
VBN |
denotes |
used |
T8438 |
32978-32981 |
VBD |
denotes |
was |
T8439 |
32987-32997 |
RB |
denotes |
throughout |
T8440 |
32998-33006 |
VBG |
denotes |
allowing |
T8441 |
33007-33018 |
NNS |
denotes |
comparisons |
T8442 |
33019-33026 |
IN |
denotes |
between |
T8443 |
33027-33031 |
JJ |
denotes |
wild |
T8444 |
33032-33036 |
NN |
denotes |
type |
T8445 |
33068-33075 |
NNS |
denotes |
samples |
T8446 |
33037-33040 |
CC |
denotes |
and |
T8447 |
33041-33046 |
NN |
denotes |
knock |
T8448 |
33047-33050 |
NN |
denotes |
out |
T8449 |
33051-33057 |
JJ |
denotes |
murine |
T8450 |
33058-33061 |
CC |
denotes |
and |
T8451 |
33062-33067 |
JJ |
denotes |
human |
T8452 |
33075-33076 |
. |
denotes |
. |
T8453 |
33076-33403 |
sentence |
denotes |
250 μl adjusted platelets were prewarmed to 37°C and used in the assay, and the aggregation process was started by adding 25 μl of a prewarmed 16.5 mg/ml ristocetin solution (DiaMed Diagnostica, Bensheim, FRG; concentration in the assay: 1.5 mg/ml), a reagent which worked reliably in our hands with mouse and human platelets. |
T8454 |
33077-33080 |
CD |
denotes |
250 |
T8455 |
33081-33083 |
NN |
denotes |
μl |
T8456 |
33093-33102 |
NNS |
denotes |
platelets |
T8457 |
33084-33092 |
VBN |
denotes |
adjusted |
T8458 |
33108-33117 |
VBN |
denotes |
prewarmed |
T8459 |
33103-33107 |
VBD |
denotes |
were |
T8460 |
33118-33120 |
IN |
denotes |
to |
T8461 |
33121-33123 |
CD |
denotes |
37 |
T8462 |
33123-33125 |
NNS |
denotes |
°C |
T8463 |
33126-33129 |
CC |
denotes |
and |
T8464 |
33130-33134 |
VBN |
denotes |
used |
T8465 |
33135-33137 |
IN |
denotes |
in |
T8466 |
33138-33141 |
DT |
denotes |
the |
T8467 |
33142-33147 |
NN |
denotes |
assay |
T8468 |
33147-33149 |
, |
denotes |
, |
T8469 |
33149-33152 |
CC |
denotes |
and |
T8470 |
33153-33156 |
DT |
denotes |
the |
T8471 |
33169-33176 |
NN |
denotes |
process |
T8472 |
33157-33168 |
NN |
denotes |
aggregation |
T8473 |
33181-33188 |
VBN |
denotes |
started |
T8474 |
33177-33180 |
VBD |
denotes |
was |
T8475 |
33189-33191 |
IN |
denotes |
by |
T8476 |
33192-33198 |
VBG |
denotes |
adding |
T8477 |
33199-33201 |
CD |
denotes |
25 |
T8478 |
33202-33204 |
NN |
denotes |
μl |
T8479 |
33205-33207 |
IN |
denotes |
of |
T8480 |
33208-33209 |
DT |
denotes |
a |
T8481 |
33242-33250 |
NN |
denotes |
solution |
T8482 |
33210-33219 |
JJ |
denotes |
prewarmed |
T8483 |
33220-33224 |
CD |
denotes |
16.5 |
T8484 |
33225-33227 |
NN |
denotes |
mg |
T8485 |
33227-33228 |
SYM |
denotes |
/ |
T8486 |
33228-33230 |
NN |
denotes |
ml |
T8487 |
33231-33241 |
NN |
denotes |
ristocetin |
T8488 |
33251-33252 |
-LRB- |
denotes |
( |
T8489 |
33282-33285 |
NNP |
denotes |
FRG |
T8490 |
33252-33258 |
NNP |
denotes |
DiaMed |
T8491 |
33259-33270 |
NNP |
denotes |
Diagnostica |
T8492 |
33270-33272 |
, |
denotes |
, |
T8493 |
33272-33280 |
NNP |
denotes |
Bensheim |
T8494 |
33280-33282 |
, |
denotes |
, |
T8495 |
33285-33286 |
: |
denotes |
; |
T8496 |
33287-33300 |
NN |
denotes |
concentration |
T8497 |
33319-33321 |
NNS |
denotes |
mg |
T8498 |
33301-33303 |
IN |
denotes |
in |
T8499 |
33304-33307 |
DT |
denotes |
the |
T8500 |
33308-33313 |
NN |
denotes |
assay |
T8501 |
33313-33315 |
: |
denotes |
: |
T8502 |
33315-33318 |
CD |
denotes |
1.5 |
T8503 |
33321-33322 |
SYM |
denotes |
/ |
T8504 |
33322-33324 |
NN |
denotes |
ml |
T8505 |
33324-33325 |
-RRB- |
denotes |
) |
T8506 |
33325-33327 |
, |
denotes |
, |
T8507 |
33327-33328 |
DT |
denotes |
a |
T8508 |
33329-33336 |
NN |
denotes |
reagent |
T8509 |
33337-33342 |
WDT |
denotes |
which |
T8510 |
33343-33349 |
VBD |
denotes |
worked |
T8511 |
33350-33358 |
RB |
denotes |
reliably |
T8512 |
33359-33361 |
IN |
denotes |
in |
T8513 |
33362-33365 |
PRP$ |
denotes |
our |
T8514 |
33366-33371 |
NNS |
denotes |
hands |
T8515 |
33372-33376 |
IN |
denotes |
with |
T8516 |
33377-33382 |
NN |
denotes |
mouse |
T8517 |
33393-33402 |
NNS |
denotes |
platelets |
T8518 |
33383-33386 |
CC |
denotes |
and |
T8519 |
33387-33392 |
JJ |
denotes |
human |
T8520 |
33402-33403 |
. |
denotes |
. |
T8521 |
33403-33452 |
sentence |
denotes |
Each murine assay represents a different animal. |
T8522 |
33404-33408 |
DT |
denotes |
Each |
T8523 |
33416-33421 |
NN |
denotes |
assay |
T8524 |
33409-33415 |
JJ |
denotes |
murine |
T8525 |
33422-33432 |
VBZ |
denotes |
represents |
T8526 |
33433-33434 |
DT |
denotes |
a |
T8527 |
33445-33451 |
NN |
denotes |
animal |
T8528 |
33435-33444 |
JJ |
denotes |
different |
T8529 |
33451-33452 |
. |
denotes |
. |
T8530 |
33452-33544 |
sentence |
denotes |
Analyses of human blood samples were performed as described in the manufacturer's protocol. |
T8531 |
33453-33461 |
NNS |
denotes |
Analyses |
T8532 |
33490-33499 |
VBN |
denotes |
performed |
T8533 |
33462-33464 |
IN |
denotes |
of |
T8534 |
33465-33470 |
JJ |
denotes |
human |
T8535 |
33477-33484 |
NNS |
denotes |
samples |
T8536 |
33471-33476 |
NN |
denotes |
blood |
T8537 |
33485-33489 |
VBD |
denotes |
were |
T8538 |
33500-33502 |
IN |
denotes |
as |
T8539 |
33503-33512 |
VBN |
denotes |
described |
T8540 |
33513-33515 |
IN |
denotes |
in |
T8541 |
33516-33519 |
DT |
denotes |
the |
T8542 |
33520-33532 |
NN |
denotes |
manufacturer |
T8543 |
33535-33543 |
NN |
denotes |
protocol |
T8544 |
33532-33534 |
POS |
denotes |
's |
T8545 |
33543-33544 |
. |
denotes |
. |
T8546 |
33544-33637 |
sentence |
denotes |
Several repeated measurements were done per human individual to control the reproducibility. |
T8547 |
33545-33552 |
JJ |
denotes |
Several |
T8548 |
33562-33574 |
NNS |
denotes |
measurements |
T8549 |
33553-33561 |
JJ |
denotes |
repeated |
T8550 |
33580-33584 |
VBN |
denotes |
done |
T8551 |
33575-33579 |
VBD |
denotes |
were |
T8552 |
33585-33588 |
IN |
denotes |
per |
T8553 |
33589-33594 |
JJ |
denotes |
human |
T8554 |
33595-33605 |
NN |
denotes |
individual |
T8555 |
33606-33608 |
TO |
denotes |
to |
T8556 |
33609-33616 |
VB |
denotes |
control |
T8557 |
33617-33620 |
DT |
denotes |
the |
T8558 |
33621-33636 |
NN |
denotes |
reproducibility |
T8559 |
33636-33637 |
. |
denotes |
. |
T8560 |
33637-33840 |
sentence |
denotes |
The acquired aggregation curve data, measured as ascending degree of transmission, were analysed for maximal slope values, while the maximal aggregation amplitude of every single curve was set to 100 %. |
T8561 |
33638-33641 |
DT |
denotes |
The |
T8562 |
33669-33673 |
NNS |
denotes |
data |
T8563 |
33642-33650 |
VBN |
denotes |
acquired |
T8564 |
33651-33662 |
NN |
denotes |
aggregation |
T8565 |
33663-33668 |
NN |
denotes |
curve |
T8566 |
33726-33734 |
VBN |
denotes |
analysed |
T8567 |
33673-33675 |
, |
denotes |
, |
T8568 |
33675-33683 |
VBN |
denotes |
measured |
T8569 |
33684-33686 |
IN |
denotes |
as |
T8570 |
33687-33696 |
VBG |
denotes |
ascending |
T8571 |
33697-33703 |
NN |
denotes |
degree |
T8572 |
33704-33706 |
IN |
denotes |
of |
T8573 |
33707-33719 |
NN |
denotes |
transmission |
T8574 |
33719-33721 |
, |
denotes |
, |
T8575 |
33721-33725 |
VBD |
denotes |
were |
T8576 |
33735-33738 |
IN |
denotes |
for |
T8577 |
33739-33746 |
JJ |
denotes |
maximal |
T8578 |
33753-33759 |
NNS |
denotes |
values |
T8579 |
33747-33752 |
NN |
denotes |
slope |
T8580 |
33759-33761 |
, |
denotes |
, |
T8581 |
33761-33766 |
IN |
denotes |
while |
T8582 |
33827-33830 |
VBN |
denotes |
set |
T8583 |
33767-33770 |
DT |
denotes |
the |
T8584 |
33791-33800 |
NN |
denotes |
amplitude |
T8585 |
33771-33778 |
JJ |
denotes |
maximal |
T8586 |
33779-33790 |
NN |
denotes |
aggregation |
T8587 |
33801-33803 |
IN |
denotes |
of |
T8588 |
33804-33809 |
DT |
denotes |
every |
T8589 |
33817-33822 |
NN |
denotes |
curve |
T8590 |
33810-33816 |
JJ |
denotes |
single |
T8591 |
33823-33826 |
VBD |
denotes |
was |
T8592 |
33831-33833 |
IN |
denotes |
to |
T8593 |
33834-33837 |
CD |
denotes |
100 |
T8594 |
33838-33839 |
NN |
denotes |
% |
T8595 |
33839-33840 |
. |
denotes |
. |
T8596 |
33840-33947 |
sentence |
denotes |
In general, all platelet procedures were done at 22°C within four hours after collecting the blood sample. |
T8597 |
33841-33843 |
IN |
denotes |
In |
T8598 |
33882-33886 |
VBN |
denotes |
done |
T8599 |
33844-33851 |
JJ |
denotes |
general |
T8600 |
33851-33853 |
, |
denotes |
, |
T8601 |
33853-33856 |
DT |
denotes |
all |
T8602 |
33866-33876 |
NNS |
denotes |
procedures |
T8603 |
33857-33865 |
NN |
denotes |
platelet |
T8604 |
33877-33881 |
VBD |
denotes |
were |
T8605 |
33887-33889 |
IN |
denotes |
at |
T8606 |
33890-33892 |
CD |
denotes |
22 |
T8607 |
33892-33894 |
NNS |
denotes |
°C |
T8608 |
33895-33901 |
IN |
denotes |
within |
T8609 |
33902-33906 |
CD |
denotes |
four |
T8610 |
33907-33912 |
NNS |
denotes |
hours |
T8611 |
33913-33918 |
IN |
denotes |
after |
T8612 |
33919-33929 |
VBG |
denotes |
collecting |
T8613 |
33930-33933 |
DT |
denotes |
the |
T8614 |
33940-33946 |
NN |
denotes |
sample |
T8615 |
33934-33939 |
NN |
denotes |
blood |
T8616 |
33946-33947 |
. |
denotes |
. |
T8735 |
33949-33962 |
JJ |
denotes |
Miscellaneous |
T8736 |
33963-33970 |
NNS |
denotes |
methods |
T8737 |
33970-34147 |
sentence |
denotes |
For standard haematopoetic measurements and cell counts on murine and human blood samples about 300 μl of blood were analysed with an ADVIA 120 electronic cell counter (Bayer). |
T8738 |
33971-33974 |
IN |
denotes |
For |
T8739 |
34088-34096 |
VBN |
denotes |
analysed |
T8740 |
33975-33983 |
JJ |
denotes |
standard |
T8741 |
33998-34010 |
NNS |
denotes |
measurements |
T8742 |
33984-33997 |
JJ |
denotes |
haematopoetic |
T8743 |
34011-34014 |
CC |
denotes |
and |
T8744 |
34015-34019 |
NN |
denotes |
cell |
T8745 |
34020-34026 |
NNS |
denotes |
counts |
T8746 |
34027-34029 |
IN |
denotes |
on |
T8747 |
34030-34036 |
JJ |
denotes |
murine |
T8748 |
34053-34060 |
NNS |
denotes |
samples |
T8749 |
34037-34040 |
CC |
denotes |
and |
T8750 |
34041-34046 |
JJ |
denotes |
human |
T8751 |
34047-34052 |
NN |
denotes |
blood |
T8752 |
34061-34066 |
IN |
denotes |
about |
T8753 |
34067-34070 |
CD |
denotes |
300 |
T8754 |
34071-34073 |
NNS |
denotes |
μl |
T8755 |
34074-34076 |
IN |
denotes |
of |
T8756 |
34077-34082 |
NN |
denotes |
blood |
T8757 |
34083-34087 |
VBD |
denotes |
were |
T8758 |
34097-34101 |
IN |
denotes |
with |
T8759 |
34102-34104 |
DT |
denotes |
an |
T8760 |
34131-34138 |
NN |
denotes |
counter |
T8761 |
34105-34110 |
NN |
denotes |
ADVIA |
T8762 |
34111-34114 |
CD |
denotes |
120 |
T8763 |
34115-34125 |
JJ |
denotes |
electronic |
T8764 |
34126-34130 |
NN |
denotes |
cell |
T8765 |
34139-34140 |
-LRB- |
denotes |
( |
T8766 |
34140-34145 |
NN |
denotes |
Bayer |
T8767 |
34145-34146 |
-RRB- |
denotes |
) |
T8768 |
34146-34147 |
. |
denotes |
. |
T8769 |
34147-34268 |
sentence |
denotes |
With this equipment red blood cells and platelets are "transformed" into spherical bodies without changing their volume. |
T8770 |
34148-34152 |
IN |
denotes |
With |
T8771 |
34203-34214 |
VBN |
denotes |
transformed |
T8772 |
34153-34157 |
DT |
denotes |
this |
T8773 |
34158-34167 |
NN |
denotes |
equipment |
T8774 |
34168-34171 |
JJ |
denotes |
red |
T8775 |
34178-34183 |
NNS |
denotes |
cells |
T8776 |
34172-34177 |
NN |
denotes |
blood |
T8777 |
34184-34187 |
CC |
denotes |
and |
T8778 |
34188-34197 |
NNS |
denotes |
platelets |
T8779 |
34198-34201 |
VBP |
denotes |
are |
T8780 |
34202-34203 |
`` |
denotes |
" |
T8781 |
34214-34215 |
'' |
denotes |
" |
T8782 |
34216-34220 |
IN |
denotes |
into |
T8783 |
34221-34230 |
JJ |
denotes |
spherical |
T8784 |
34231-34237 |
NNS |
denotes |
bodies |
T8785 |
34238-34245 |
IN |
denotes |
without |
T8786 |
34246-34254 |
VBG |
denotes |
changing |
T8787 |
34255-34260 |
PRP$ |
denotes |
their |
T8788 |
34261-34267 |
NN |
denotes |
volume |
T8789 |
34267-34268 |
. |
denotes |
. |
T8790 |
34268-34386 |
sentence |
denotes |
Therefore number and volume of red blood cells and platelets are not calculated values, but directly measured values. |
T8791 |
34269-34278 |
RB |
denotes |
Therefore |
T8792 |
34330-34333 |
VBP |
denotes |
are |
T8793 |
34279-34285 |
NN |
denotes |
number |
T8794 |
34286-34289 |
CC |
denotes |
and |
T8795 |
34290-34296 |
NN |
denotes |
volume |
T8796 |
34297-34299 |
IN |
denotes |
of |
T8797 |
34300-34303 |
JJ |
denotes |
red |
T8798 |
34310-34315 |
NNS |
denotes |
cells |
T8799 |
34304-34309 |
NN |
denotes |
blood |
T8800 |
34316-34319 |
CC |
denotes |
and |
T8801 |
34320-34329 |
NNS |
denotes |
platelets |
T8802 |
34334-34337 |
RB |
denotes |
not |
T8803 |
34338-34348 |
VBN |
denotes |
calculated |
T8804 |
34349-34355 |
NNS |
denotes |
values |
T8805 |
34355-34357 |
, |
denotes |
, |
T8806 |
34357-34360 |
CC |
denotes |
but |
T8807 |
34361-34369 |
RB |
denotes |
directly |
T8808 |
34370-34378 |
VBN |
denotes |
measured |
T8809 |
34379-34385 |
NNS |
denotes |
values |
T8810 |
34385-34386 |
. |
denotes |
. |
T8811 |
34386-34660 |
sentence |
denotes |
The following data were statistically analysed: leucocytes, red blood cells, haemoglobin, haematocrit, mean cellular volume, mean cellular haemoglobin, mean cellular haemoglobin concentration, platelet number, neutrophiles, lymphocytes, monocytes, eosinophiles, basophiles. |
T8812 |
34387-34390 |
DT |
denotes |
The |
T8813 |
34401-34405 |
NNS |
denotes |
data |
T8814 |
34391-34400 |
VBG |
denotes |
following |
T8815 |
34425-34433 |
VBN |
denotes |
analysed |
T8816 |
34406-34410 |
VBD |
denotes |
were |
T8817 |
34411-34424 |
RB |
denotes |
statistically |
T8818 |
34433-34435 |
: |
denotes |
: |
T8819 |
34435-34445 |
NNS |
denotes |
leucocytes |
T8820 |
34445-34447 |
, |
denotes |
, |
T8821 |
34447-34450 |
JJ |
denotes |
red |
T8822 |
34457-34462 |
NNS |
denotes |
cells |
T8823 |
34451-34456 |
NN |
denotes |
blood |
T8824 |
34462-34464 |
, |
denotes |
, |
T8825 |
34464-34475 |
NN |
denotes |
haemoglobin |
T8826 |
34475-34477 |
, |
denotes |
, |
T8827 |
34477-34488 |
NN |
denotes |
haematocrit |
T8828 |
34488-34490 |
, |
denotes |
, |
T8829 |
34490-34494 |
JJ |
denotes |
mean |
T8830 |
34504-34510 |
NN |
denotes |
volume |
T8831 |
34495-34503 |
JJ |
denotes |
cellular |
T8832 |
34510-34512 |
, |
denotes |
, |
T8833 |
34512-34516 |
JJ |
denotes |
mean |
T8834 |
34526-34537 |
NN |
denotes |
haemoglobin |
T8835 |
34517-34525 |
JJ |
denotes |
cellular |
T8836 |
34537-34539 |
, |
denotes |
, |
T8837 |
34539-34543 |
JJ |
denotes |
mean |
T8838 |
34553-34564 |
NN |
denotes |
haemoglobin |
T8839 |
34544-34552 |
JJ |
denotes |
cellular |
T8840 |
34565-34578 |
NN |
denotes |
concentration |
T8841 |
34578-34580 |
, |
denotes |
, |
T8842 |
34580-34588 |
NN |
denotes |
platelet |
T8843 |
34589-34595 |
NN |
denotes |
number |
T8844 |
34595-34597 |
, |
denotes |
, |
T8845 |
34597-34609 |
NNS |
denotes |
neutrophiles |
T8846 |
34609-34611 |
, |
denotes |
, |
T8847 |
34611-34622 |
NNS |
denotes |
lymphocytes |
T8848 |
34622-34624 |
, |
denotes |
, |
T8849 |
34624-34633 |
NNS |
denotes |
monocytes |
T8850 |
34633-34635 |
, |
denotes |
, |
T8851 |
34635-34647 |
NNS |
denotes |
eosinophiles |
T8852 |
34647-34649 |
, |
denotes |
, |
T8853 |
34649-34659 |
NNS |
denotes |
basophiles |
T8854 |
34659-34660 |
. |
denotes |
. |
T8855 |
34660-34753 |
sentence |
denotes |
Peptide mass fingerprinting was performed on a Bruker Reflex IV MALDI-TOF mass spectrometer. |
T8856 |
34661-34668 |
NN |
denotes |
Peptide |
T8857 |
34669-34673 |
NN |
denotes |
mass |
T8858 |
34674-34688 |
NN |
denotes |
fingerprinting |
T8859 |
34693-34702 |
VBN |
denotes |
performed |
T8860 |
34689-34692 |
VBD |
denotes |
was |
T8861 |
34703-34705 |
IN |
denotes |
on |
T8862 |
34706-34707 |
DT |
denotes |
a |
T8863 |
34740-34752 |
NN |
denotes |
spectrometer |
T8864 |
34708-34714 |
NNP |
denotes |
Bruker |
T8865 |
34715-34721 |
NNP |
denotes |
Reflex |
T8866 |
34722-34724 |
CD |
denotes |
IV |
T8867 |
34725-34730 |
NN |
denotes |
MALDI |
T8868 |
34731-34734 |
NN |
denotes |
TOF |
T8869 |
34730-34731 |
HYPH |
denotes |
- |
T8870 |
34735-34739 |
NN |
denotes |
mass |
T8871 |
34752-34753 |
. |
denotes |
. |
T8872 |
34753-34878 |
sentence |
denotes |
Recombinant annexin A7 was purified according to [27], F-actin-binding of recombinant annexin A7 was done as described [28]. |
T8873 |
34754-34765 |
JJ |
denotes |
Recombinant |
T8874 |
34774-34776 |
NN |
denotes |
A7 |
T8875 |
34766-34773 |
NN |
denotes |
annexin |
T8876 |
34781-34789 |
VBN |
denotes |
purified |
T8877 |
34777-34780 |
VBD |
denotes |
was |
T8878 |
34855-34859 |
VBN |
denotes |
done |
T8879 |
34790-34799 |
VBG |
denotes |
according |
T8880 |
34800-34802 |
IN |
denotes |
to |
T8881 |
34803-34804 |
-LRB- |
denotes |
[ |
T8882 |
34804-34806 |
CD |
denotes |
27 |
T8883 |
34806-34807 |
-RRB- |
denotes |
] |
T8884 |
34807-34809 |
, |
denotes |
, |
T8885 |
34809-34810 |
NN |
denotes |
F |
T8886 |
34811-34816 |
NN |
denotes |
actin |
T8887 |
34810-34811 |
HYPH |
denotes |
- |
T8888 |
34816-34817 |
HYPH |
denotes |
- |
T8889 |
34817-34824 |
VBG |
denotes |
binding |
T8890 |
34825-34827 |
IN |
denotes |
of |
T8891 |
34828-34839 |
JJ |
denotes |
recombinant |
T8892 |
34848-34850 |
NN |
denotes |
A7 |
T8893 |
34840-34847 |
NN |
denotes |
annexin |
T8894 |
34851-34854 |
VBD |
denotes |
was |
T8895 |
34860-34862 |
IN |
denotes |
as |
T8896 |
34863-34872 |
VBN |
denotes |
described |
T8897 |
34873-34874 |
-LRB- |
denotes |
[ |
T8898 |
34874-34876 |
CD |
denotes |
28 |
T8899 |
34876-34877 |
-RRB- |
denotes |
] |
T8900 |
34877-34878 |
. |
denotes |
. |
T8901 |
34878-34940 |
sentence |
denotes |
SDS-PAGE and western blotting were done as described [29,30]. |
T8902 |
34879-34882 |
NN |
denotes |
SDS |
T8903 |
34883-34887 |
NN |
denotes |
PAGE |
T8904 |
34882-34883 |
HYPH |
denotes |
- |
T8905 |
34914-34918 |
VBN |
denotes |
done |
T8906 |
34888-34891 |
CC |
denotes |
and |
T8907 |
34892-34899 |
JJ |
denotes |
western |
T8908 |
34900-34908 |
NN |
denotes |
blotting |
T8909 |
34909-34913 |
VBD |
denotes |
were |
T8910 |
34919-34921 |
IN |
denotes |
as |
T8911 |
34922-34931 |
VBN |
denotes |
described |
T8912 |
34932-34933 |
-LRB- |
denotes |
[ |
T8913 |
34936-34938 |
CD |
denotes |
30 |
T8914 |
34933-34935 |
CD |
denotes |
29 |
T8915 |
34935-34936 |
, |
denotes |
, |
T8916 |
34938-34939 |
-RRB- |
denotes |
] |
T8917 |
34939-34940 |
. |
denotes |
. |
T8918 |
34940-35007 |
sentence |
denotes |
Detection in immunoblots was with enhanced chemiluminescence [17]. |
T8919 |
34941-34950 |
NN |
denotes |
Detection |
T8920 |
34966-34969 |
VBD |
denotes |
was |
T8921 |
34951-34953 |
IN |
denotes |
in |
T8922 |
34954-34965 |
NNS |
denotes |
immunoblots |
T8923 |
34970-34974 |
IN |
denotes |
with |
T8924 |
34975-34983 |
VBN |
denotes |
enhanced |
T8925 |
34984-35001 |
NN |
denotes |
chemiluminescence |
T8926 |
35002-35003 |
-LRB- |
denotes |
[ |
T8927 |
35003-35005 |
CD |
denotes |
17 |
T8928 |
35005-35006 |
-RRB- |
denotes |
] |
T8929 |
35006-35007 |
. |
denotes |
. |
T8930 |
35007-35145 |
sentence |
denotes |
Antibodies employed were mab 203–217 directed against mouse annexin A7 core domain [5] and a polyclonal rabbit anti-sorcin antibody [31]. |
T8931 |
35008-35018 |
NNS |
denotes |
Antibodies |
T8932 |
35028-35032 |
VBD |
denotes |
were |
T8933 |
35019-35027 |
VBN |
denotes |
employed |
T8934 |
35033-35036 |
NN |
denotes |
mab |
T8935 |
35037-35040 |
CD |
denotes |
203 |
T8936 |
35040-35041 |
SYM |
denotes |
– |
T8937 |
35041-35044 |
CD |
denotes |
217 |
T8938 |
35045-35053 |
VBN |
denotes |
directed |
T8939 |
35054-35061 |
IN |
denotes |
against |
T8940 |
35062-35067 |
NN |
denotes |
mouse |
T8941 |
35084-35090 |
NN |
denotes |
domain |
T8942 |
35068-35075 |
NN |
denotes |
annexin |
T8943 |
35076-35078 |
NN |
denotes |
A7 |
T8944 |
35079-35083 |
NN |
denotes |
core |
T8945 |
35091-35092 |
-LRB- |
denotes |
[ |
T8946 |
35092-35093 |
CD |
denotes |
5 |
T8947 |
35093-35094 |
-RRB- |
denotes |
] |
T8948 |
35095-35098 |
CC |
denotes |
and |
T8949 |
35099-35100 |
DT |
denotes |
a |
T8950 |
35131-35139 |
NN |
denotes |
antibody |
T8951 |
35101-35111 |
JJ |
denotes |
polyclonal |
T8952 |
35112-35118 |
NN |
denotes |
rabbit |
T8953 |
35119-35130 |
JJ |
denotes |
anti-sorcin |
T8954 |
35140-35141 |
-LRB- |
denotes |
[ |
T8955 |
35141-35143 |
CD |
denotes |
31 |
T8956 |
35143-35144 |
-RRB- |
denotes |
] |
T8957 |
35144-35145 |
. |
denotes |
. |
T8958 |
35145-35462 |
sentence |
denotes |
For statistical analyses the following tests were performed: David et al. range test to ensure samples are from a normal distribution, F-test looking for a samples' homoscedascity, Student's T-test to judge if samples of different groups have a different mean value and are parts from different normal distributions. |
T8959 |
35146-35149 |
IN |
denotes |
For |
T8960 |
35196-35205 |
VBN |
denotes |
performed |
T8961 |
35150-35161 |
JJ |
denotes |
statistical |
T8962 |
35162-35170 |
NNS |
denotes |
analyses |
T8963 |
35171-35174 |
DT |
denotes |
the |
T8964 |
35185-35190 |
NNS |
denotes |
tests |
T8965 |
35175-35184 |
VBG |
denotes |
following |
T8966 |
35191-35195 |
VBD |
denotes |
were |
T8967 |
35205-35207 |
: |
denotes |
: |
T8968 |
35207-35212 |
NNP |
denotes |
David |
T8969 |
35226-35230 |
NN |
denotes |
test |
T8970 |
35213-35215 |
FW |
denotes |
et |
T8971 |
35216-35219 |
FW |
denotes |
al. |
T8972 |
35220-35225 |
NN |
denotes |
range |
T8973 |
35231-35233 |
TO |
denotes |
to |
T8974 |
35234-35240 |
VB |
denotes |
ensure |
T8975 |
35241-35248 |
NNS |
denotes |
samples |
T8976 |
35249-35252 |
VBP |
denotes |
are |
T8977 |
35253-35257 |
IN |
denotes |
from |
T8978 |
35258-35259 |
DT |
denotes |
a |
T8979 |
35267-35279 |
NN |
denotes |
distribution |
T8980 |
35260-35266 |
JJ |
denotes |
normal |
T8981 |
35279-35281 |
, |
denotes |
, |
T8982 |
35281-35282 |
NN |
denotes |
F |
T8983 |
35283-35287 |
NN |
denotes |
test |
T8984 |
35282-35283 |
HYPH |
denotes |
- |
T8985 |
35288-35295 |
VBG |
denotes |
looking |
T8986 |
35296-35299 |
IN |
denotes |
for |
T8987 |
35300-35301 |
DT |
denotes |
a |
T8988 |
35302-35309 |
NNS |
denotes |
samples |
T8989 |
35311-35325 |
NN |
denotes |
homoscedascity |
T8990 |
35309-35310 |
POS |
denotes |
' |
T8991 |
35325-35327 |
, |
denotes |
, |
T8992 |
35327-35334 |
NN |
denotes |
Student |
T8993 |
35339-35343 |
NN |
denotes |
test |
T8994 |
35334-35336 |
POS |
denotes |
's |
T8995 |
35337-35338 |
NN |
denotes |
T |
T8996 |
35338-35339 |
HYPH |
denotes |
- |
T8997 |
35344-35346 |
TO |
denotes |
to |
T8998 |
35347-35352 |
VB |
denotes |
judge |
T8999 |
35353-35355 |
IN |
denotes |
if |
T9000 |
35384-35388 |
VBP |
denotes |
have |
T9001 |
35356-35363 |
NNS |
denotes |
samples |
T9002 |
35364-35366 |
IN |
denotes |
of |
T9003 |
35367-35376 |
JJ |
denotes |
different |
T9004 |
35377-35383 |
NNS |
denotes |
groups |
T9005 |
35389-35390 |
DT |
denotes |
a |
T9006 |
35406-35411 |
NN |
denotes |
value |
T9007 |
35391-35400 |
JJ |
denotes |
different |
T9008 |
35401-35405 |
JJ |
denotes |
mean |
T9009 |
35412-35415 |
CC |
denotes |
and |
T9010 |
35416-35419 |
VBP |
denotes |
are |
T9011 |
35420-35425 |
NNS |
denotes |
parts |
T9012 |
35426-35430 |
IN |
denotes |
from |
T9013 |
35431-35440 |
JJ |
denotes |
different |
T9014 |
35448-35461 |
NNS |
denotes |
distributions |
T9015 |
35441-35447 |
JJ |
denotes |
normal |
T9016 |
35461-35462 |
. |
denotes |
. |
T9017 |
35462-35577 |
sentence |
denotes |
The exact probability values and the significance of an analysis are indicated when the experiments are described. |
T9018 |
35463-35466 |
DT |
denotes |
The |
T9019 |
35485-35491 |
NNS |
denotes |
values |
T9020 |
35467-35472 |
JJ |
denotes |
exact |
T9021 |
35473-35484 |
NN |
denotes |
probability |
T9022 |
35532-35541 |
VBN |
denotes |
indicated |
T9023 |
35492-35495 |
CC |
denotes |
and |
T9024 |
35496-35499 |
DT |
denotes |
the |
T9025 |
35500-35512 |
NN |
denotes |
significance |
T9026 |
35513-35515 |
IN |
denotes |
of |
T9027 |
35516-35518 |
DT |
denotes |
an |
T9028 |
35519-35527 |
NN |
denotes |
analysis |
T9029 |
35528-35531 |
VBP |
denotes |
are |
T9030 |
35542-35546 |
WRB |
denotes |
when |
T9031 |
35567-35576 |
VBN |
denotes |
described |
T9032 |
35547-35550 |
DT |
denotes |
the |
T9033 |
35551-35562 |
NNS |
denotes |
experiments |
T9034 |
35563-35566 |
VBP |
denotes |
are |
T9035 |
35576-35577 |
. |
denotes |
. |
T9089 |
7932-7942 |
NN |
denotes |
Expression |
T9090 |
7943-7945 |
IN |
denotes |
of |
T9091 |
7946-7953 |
NN |
denotes |
annexin |
T9092 |
7954-7956 |
NN |
denotes |
A7 |
T9093 |
7957-7959 |
IN |
denotes |
in |
T9094 |
7960-7965 |
JJ |
denotes |
human |
T9095 |
7976-7981 |
NNS |
denotes |
cells |
T9096 |
7966-7969 |
JJ |
denotes |
red |
T9097 |
7970-7975 |
NN |
denotes |
blood |
T9098 |
7982-7983 |
-LRB- |
denotes |
( |
T9099 |
7983-7984 |
CD |
denotes |
1 |
T9100 |
7984-7985 |
-RRB- |
denotes |
) |
T9101 |
7986-7989 |
CC |
denotes |
and |
T9102 |
7990-7995 |
JJ |
denotes |
human |
T9103 |
7996-8005 |
NNS |
denotes |
platelets |
T9104 |
8006-8007 |
-LRB- |
denotes |
( |
T9105 |
8007-8008 |
CD |
denotes |
2 |
T9106 |
8008-8009 |
-RRB- |
denotes |
) |
T9107 |
8009-8010 |
. |
denotes |
. |
T9108 |
8010-8076 |
sentence |
denotes |
Protein homogenates were separated by SDS-PAGE (12 % acrylamide). |
T9109 |
8011-8018 |
NN |
denotes |
Protein |
T9110 |
8019-8030 |
NNS |
denotes |
homogenates |
T9111 |
8036-8045 |
VBN |
denotes |
separated |
T9112 |
8031-8035 |
VBD |
denotes |
were |
T9113 |
8046-8048 |
IN |
denotes |
by |
T9114 |
8049-8052 |
NN |
denotes |
SDS |
T9115 |
8053-8057 |
NN |
denotes |
PAGE |
T9116 |
8052-8053 |
HYPH |
denotes |
- |
T9117 |
8058-8059 |
-LRB- |
denotes |
( |
T9118 |
8064-8074 |
NN |
denotes |
acrylamide |
T9119 |
8059-8061 |
CD |
denotes |
12 |
T9120 |
8062-8063 |
NN |
denotes |
% |
T9121 |
8074-8075 |
-RRB- |
denotes |
) |
T9122 |
8075-8076 |
. |
denotes |
. |
T9123 |
8076-8210 |
sentence |
denotes |
The resulting western blot was probed with mAb 203–217, visualization was by a secondary peroxidase coupled antibody followed by ECL. |
T9124 |
8077-8080 |
DT |
denotes |
The |
T9125 |
8099-8103 |
NN |
denotes |
blot |
T9126 |
8081-8090 |
VBG |
denotes |
resulting |
T9127 |
8091-8098 |
JJ |
denotes |
western |
T9128 |
8108-8114 |
VBN |
denotes |
probed |
T9129 |
8104-8107 |
VBD |
denotes |
was |
T9130 |
8147-8150 |
VBD |
denotes |
was |
T9131 |
8115-8119 |
IN |
denotes |
with |
T9132 |
8120-8123 |
NN |
denotes |
mAb |
T9133 |
8124-8131 |
CD |
denotes |
203–217 |
T9134 |
8131-8133 |
, |
denotes |
, |
T9135 |
8133-8146 |
NN |
denotes |
visualization |
T9136 |
8151-8153 |
IN |
denotes |
by |
T9137 |
8154-8155 |
DT |
denotes |
a |
T9138 |
8185-8193 |
NN |
denotes |
antibody |
T9139 |
8156-8165 |
JJ |
denotes |
secondary |
T9140 |
8166-8176 |
NN |
denotes |
peroxidase |
T9141 |
8177-8184 |
VBN |
denotes |
coupled |
T9142 |
8194-8202 |
VBN |
denotes |
followed |
T9143 |
8203-8205 |
IN |
denotes |
by |
T9144 |
8206-8209 |
NN |
denotes |
ECL |
T9145 |
8209-8210 |
. |
denotes |
. |
T9146 |
8210-8305 |
sentence |
denotes |
Both isoforms are detected in red blood cells, in platelets only the small isoform is present. |
T9147 |
8211-8215 |
DT |
denotes |
Both |
T9148 |
8216-8224 |
NNS |
denotes |
isoforms |
T9149 |
8229-8237 |
VBN |
denotes |
detected |
T9150 |
8225-8228 |
VBP |
denotes |
are |
T9151 |
8294-8296 |
VBZ |
denotes |
is |
T9152 |
8238-8240 |
IN |
denotes |
in |
T9153 |
8241-8244 |
JJ |
denotes |
red |
T9154 |
8251-8256 |
NNS |
denotes |
cells |
T9155 |
8245-8250 |
NN |
denotes |
blood |
T9156 |
8256-8258 |
, |
denotes |
, |
T9157 |
8258-8260 |
IN |
denotes |
in |
T9158 |
8261-8270 |
NNS |
denotes |
platelets |
T9159 |
8271-8275 |
RB |
denotes |
only |
T9160 |
8286-8293 |
NN |
denotes |
isoform |
T9161 |
8276-8279 |
DT |
denotes |
the |
T9162 |
8280-8285 |
JJ |
denotes |
small |
T9163 |
8297-8304 |
JJ |
denotes |
present |
T9164 |
8304-8305 |
. |
denotes |
. |
T9240 |
9648-9658 |
NN |
denotes |
Enrichment |
T9241 |
9659-9661 |
IN |
denotes |
of |
T9242 |
9662-9669 |
NN |
denotes |
annexin |
T9243 |
9670-9672 |
NN |
denotes |
A7 |
T9244 |
9673-9676 |
CC |
denotes |
and |
T9245 |
9677-9683 |
NN |
denotes |
sorcin |
T9246 |
9684-9686 |
IN |
denotes |
in |
T9247 |
9687-9698 |
NNS |
denotes |
exovesicles |
T9248 |
9699-9706 |
VBN |
denotes |
derived |
T9249 |
9707-9711 |
IN |
denotes |
from |
T9250 |
9712-9715 |
JJ |
denotes |
red |
T9251 |
9722-9727 |
NNS |
denotes |
cells |
T9252 |
9716-9721 |
NN |
denotes |
blood |
T9253 |
9727-9728 |
. |
denotes |
. |
T9254 |
9728-9950 |
sentence |
denotes |
Vesicles from wild type (wt) and anxA7-/- mutant (ko) were generated with Ca2+/ionophore treatment, isolated by differential centrifugation and analysed by immunoblotting with mAb 203–217 and a sorcin polyclonal antibody. |
T9255 |
9729-9737 |
NNS |
denotes |
Vesicles |
T9256 |
9788-9797 |
VBN |
denotes |
generated |
T9257 |
9738-9742 |
IN |
denotes |
from |
T9258 |
9743-9747 |
JJ |
denotes |
wild |
T9259 |
9748-9752 |
NN |
denotes |
type |
T9260 |
9753-9754 |
-LRB- |
denotes |
( |
T9261 |
9754-9756 |
NN |
denotes |
wt |
T9262 |
9756-9757 |
-RRB- |
denotes |
) |
T9263 |
9758-9761 |
CC |
denotes |
and |
T9264 |
9762-9767 |
JJ |
denotes |
anxA7 |
T9265 |
9771-9777 |
NN |
denotes |
mutant |
T9266 |
9767-9768 |
SYM |
denotes |
- |
T9267 |
9768-9769 |
HYPH |
denotes |
/ |
T9268 |
9769-9770 |
SYM |
denotes |
- |
T9269 |
9778-9779 |
-LRB- |
denotes |
( |
T9270 |
9779-9781 |
NN |
denotes |
ko |
T9271 |
9781-9782 |
-RRB- |
denotes |
) |
T9272 |
9783-9787 |
VBD |
denotes |
were |
T9273 |
9798-9802 |
IN |
denotes |
with |
T9274 |
9803-9807 |
NN |
denotes |
Ca2+ |
T9275 |
9808-9817 |
NN |
denotes |
ionophore |
T9276 |
9807-9808 |
HYPH |
denotes |
/ |
T9277 |
9818-9827 |
NN |
denotes |
treatment |
T9278 |
9827-9829 |
, |
denotes |
, |
T9279 |
9829-9837 |
VBN |
denotes |
isolated |
T9280 |
9838-9840 |
IN |
denotes |
by |
T9281 |
9841-9853 |
JJ |
denotes |
differential |
T9282 |
9854-9868 |
NN |
denotes |
centrifugation |
T9283 |
9869-9872 |
CC |
denotes |
and |
T9284 |
9873-9881 |
VBN |
denotes |
analysed |
T9285 |
9882-9884 |
IN |
denotes |
by |
T9286 |
9885-9899 |
VBG |
denotes |
immunoblotting |
T9287 |
9900-9904 |
IN |
denotes |
with |
T9288 |
9905-9908 |
NN |
denotes |
mAb |
T9289 |
9909-9912 |
CD |
denotes |
203 |
T9290 |
9912-9913 |
SYM |
denotes |
– |
T9291 |
9913-9916 |
CD |
denotes |
217 |
T9292 |
9917-9920 |
CC |
denotes |
and |
T9293 |
9921-9922 |
DT |
denotes |
a |
T9294 |
9941-9949 |
NN |
denotes |
antibody |
T9295 |
9923-9929 |
NN |
denotes |
sorcin |
T9296 |
9930-9940 |
JJ |
denotes |
polyclonal |
T9297 |
9949-9950 |
. |
denotes |
. |
T9298 |
9950-10019 |
sentence |
denotes |
In general, annexin A7 and sorcin are more abundant in nanovesicles. |
T9299 |
9951-9953 |
IN |
denotes |
In |
T9300 |
9985-9988 |
VBP |
denotes |
are |
T9301 |
9954-9961 |
JJ |
denotes |
general |
T9302 |
9961-9963 |
, |
denotes |
, |
T9303 |
9963-9970 |
NN |
denotes |
annexin |
T9304 |
9971-9973 |
NN |
denotes |
A7 |
T9305 |
9974-9977 |
CC |
denotes |
and |
T9306 |
9978-9984 |
NN |
denotes |
sorcin |
T9307 |
9989-9993 |
RBR |
denotes |
more |
T9308 |
9994-10002 |
JJ |
denotes |
abundant |
T9309 |
10003-10005 |
IN |
denotes |
in |
T9310 |
10006-10018 |
NNS |
denotes |
nanovesicles |
T9311 |
10018-10019 |
. |
denotes |
. |
T9312 |
10019-10095 |
sentence |
denotes |
The 51 kDa isoform of annexin A7 (arrows) is only observed in nanovesicles. |
T9313 |
10020-10023 |
DT |
denotes |
The |
T9314 |
10031-10038 |
NN |
denotes |
isoform |
T9315 |
10024-10026 |
CD |
denotes |
51 |
T9316 |
10027-10030 |
NN |
denotes |
kDa |
T9317 |
10070-10078 |
VBN |
denotes |
observed |
T9318 |
10039-10041 |
IN |
denotes |
of |
T9319 |
10042-10049 |
NN |
denotes |
annexin |
T9320 |
10050-10052 |
NN |
denotes |
A7 |
T9321 |
10053-10054 |
-LRB- |
denotes |
( |
T9322 |
10054-10060 |
NNS |
denotes |
arrows |
T9323 |
10060-10061 |
-RRB- |
denotes |
) |
T9324 |
10062-10064 |
VBZ |
denotes |
is |
T9325 |
10065-10069 |
RB |
denotes |
only |
T9326 |
10079-10081 |
IN |
denotes |
in |
T9327 |
10082-10094 |
NNS |
denotes |
nanovesicles |
T9328 |
10094-10095 |
. |
denotes |
. |
T9329 |
10095-10194 |
sentence |
denotes |
Samples of both vesicles types were normalized according to their acetylcholine esterase activity. |
T9330 |
10096-10103 |
NNS |
denotes |
Samples |
T9331 |
10132-10142 |
VBN |
denotes |
normalized |
T9332 |
10104-10106 |
IN |
denotes |
of |
T9333 |
10107-10111 |
DT |
denotes |
both |
T9334 |
10121-10126 |
NNS |
denotes |
types |
T9335 |
10112-10120 |
NNS |
denotes |
vesicles |
T9336 |
10127-10131 |
VBD |
denotes |
were |
T9337 |
10143-10152 |
VBG |
denotes |
according |
T9338 |
10153-10155 |
IN |
denotes |
to |
T9339 |
10156-10161 |
PRP$ |
denotes |
their |
T9340 |
10185-10193 |
NN |
denotes |
activity |
T9341 |
10162-10175 |
NN |
denotes |
acetylcholine |
T9342 |
10176-10184 |
NN |
denotes |
esterase |
T9343 |
10193-10194 |
. |
denotes |
. |
T9344 |
10194-10273 |
sentence |
denotes |
Human red blood cells (co) were used for control and normalized independently. |
T9345 |
10195-10200 |
JJ |
denotes |
Human |
T9346 |
10211-10216 |
NNS |
denotes |
cells |
T9347 |
10201-10204 |
JJ |
denotes |
red |
T9348 |
10205-10210 |
NN |
denotes |
blood |
T9349 |
10227-10231 |
VBN |
denotes |
used |
T9350 |
10217-10218 |
-LRB- |
denotes |
( |
T9351 |
10218-10220 |
NN |
denotes |
co |
T9352 |
10220-10221 |
-RRB- |
denotes |
) |
T9353 |
10222-10226 |
VBD |
denotes |
were |
T9354 |
10232-10235 |
IN |
denotes |
for |
T9355 |
10236-10243 |
NN |
denotes |
control |
T9356 |
10244-10247 |
CC |
denotes |
and |
T9357 |
10248-10258 |
VBD |
denotes |
normalized |
T9358 |
10259-10272 |
RB |
denotes |
independently |
T9359 |
10272-10273 |
. |
denotes |
. |
T9397 |
11121-11132 |
NN |
denotes |
Association |
T9398 |
11133-11135 |
IN |
denotes |
of |
T9399 |
11136-11143 |
NN |
denotes |
annexin |
T9400 |
11144-11146 |
NN |
denotes |
A7 |
T9401 |
11147-11150 |
CC |
denotes |
and |
T9402 |
11151-11157 |
NN |
denotes |
sorcin |
T9403 |
11158-11162 |
IN |
denotes |
with |
T9404 |
11163-11171 |
JJ |
denotes |
distinct |
T9405 |
11203-11212 |
NNS |
denotes |
fractions |
T9406 |
11172-11175 |
JJ |
denotes |
red |
T9407 |
11182-11186 |
NN |
denotes |
cell |
T9408 |
11176-11181 |
NN |
denotes |
blood |
T9409 |
11187-11193 |
NN |
denotes |
plasma |
T9410 |
11194-11202 |
NN |
denotes |
membrane |
T9411 |
11212-11213 |
. |
denotes |
. |
T9412 |
11213-11298 |
sentence |
denotes |
Lysed human red blood cells were added to a self forming iodixanol density gradient. |
T9413 |
11214-11219 |
VBN |
denotes |
Lysed |
T9414 |
11236-11241 |
NNS |
denotes |
cells |
T9415 |
11220-11225 |
JJ |
denotes |
human |
T9416 |
11226-11229 |
JJ |
denotes |
red |
T9417 |
11230-11235 |
NN |
denotes |
blood |
T9418 |
11247-11252 |
VBN |
denotes |
added |
T9419 |
11242-11246 |
VBD |
denotes |
were |
T9420 |
11253-11255 |
IN |
denotes |
to |
T9421 |
11256-11257 |
DT |
denotes |
a |
T9422 |
11289-11297 |
NN |
denotes |
gradient |
T9423 |
11258-11262 |
NN |
denotes |
self |
T9424 |
11263-11270 |
VBG |
denotes |
forming |
T9425 |
11271-11280 |
NN |
denotes |
iodixanol |
T9426 |
11281-11288 |
NN |
denotes |
density |
T9427 |
11297-11298 |
. |
denotes |
. |
T9428 |
11298-11380 |
sentence |
denotes |
The density of the gradient increases from left (1.06 g/ml) to right (1.20 g/ml). |
T9429 |
11299-11302 |
DT |
denotes |
The |
T9430 |
11303-11310 |
NN |
denotes |
density |
T9431 |
11327-11336 |
VBZ |
denotes |
increases |
T9432 |
11311-11313 |
IN |
denotes |
of |
T9433 |
11314-11317 |
DT |
denotes |
the |
T9434 |
11318-11326 |
NN |
denotes |
gradient |
T9435 |
11337-11341 |
IN |
denotes |
from |
T9436 |
11342-11346 |
JJ |
denotes |
left |
T9437 |
11347-11348 |
-LRB- |
denotes |
( |
T9438 |
11353-11354 |
NNS |
denotes |
g |
T9439 |
11348-11352 |
CD |
denotes |
1.06 |
T9440 |
11354-11355 |
SYM |
denotes |
/ |
T9441 |
11355-11357 |
NN |
denotes |
ml |
T9442 |
11357-11358 |
-RRB- |
denotes |
) |
T9443 |
11359-11361 |
IN |
denotes |
to |
T9444 |
11362-11367 |
JJ |
denotes |
right |
T9445 |
11368-11369 |
-LRB- |
denotes |
( |
T9446 |
11374-11375 |
NNS |
denotes |
g |
T9447 |
11369-11373 |
CD |
denotes |
1.20 |
T9448 |
11375-11376 |
SYM |
denotes |
/ |
T9449 |
11376-11378 |
NN |
denotes |
ml |
T9450 |
11378-11379 |
-RRB- |
denotes |
) |
T9451 |
11379-11380 |
. |
denotes |
. |
T9452 |
11380-11476 |
sentence |
denotes |
Fractions were analysed by immunoblotting using mAb 203–217 and the sorcin polyclonal antibody. |
T9453 |
11381-11390 |
NNS |
denotes |
Fractions |
T9454 |
11396-11404 |
VBN |
denotes |
analysed |
T9455 |
11391-11395 |
VBD |
denotes |
were |
T9456 |
11405-11407 |
IN |
denotes |
by |
T9457 |
11408-11422 |
NN |
denotes |
immunoblotting |
T9458 |
11423-11428 |
VBG |
denotes |
using |
T9459 |
11429-11432 |
NN |
denotes |
mAb |
T9460 |
11433-11436 |
CD |
denotes |
203 |
T9461 |
11436-11437 |
SYM |
denotes |
– |
T9462 |
11437-11440 |
CD |
denotes |
217 |
T9463 |
11441-11444 |
CC |
denotes |
and |
T9464 |
11445-11448 |
DT |
denotes |
the |
T9465 |
11467-11475 |
NN |
denotes |
antibody |
T9466 |
11449-11455 |
NN |
denotes |
sorcin |
T9467 |
11456-11466 |
JJ |
denotes |
polyclonal |
T9468 |
11475-11476 |
. |
denotes |
. |
T9533 |
12430-12441 |
JJ |
denotes |
significant |
T9534 |
12442-12443 |
-LRB- |
denotes |
( |
T9535 |
12458-12460 |
CD |
denotes |
40 |
T9536 |
12443-12444 |
NN |
denotes |
p |
T9537 |
12447-12452 |
CD |
denotes |
0.015 |
T9538 |
12445-12446 |
SYM |
denotes |
= |
T9539 |
12452-12453 |
: |
denotes |
; |
T9540 |
12454-12455 |
NN |
denotes |
n |
T9541 |
12456-12457 |
SYM |
denotes |
= |
T9542 |
12460-12461 |
-RRB- |
denotes |
) |
T9543 |
12461-12462 |
. |
denotes |
. |
T9544 |
12462-12473 |
sentence |
denotes |
Bar, 3 μm. |
T9545 |
12463-12466 |
NN |
denotes |
Bar |
T9546 |
12470-12472 |
NNS |
denotes |
μm |
T9547 |
12466-12468 |
, |
denotes |
, |
T9548 |
12468-12469 |
CD |
denotes |
3 |
T9549 |
12472-12473 |
. |
denotes |
. |
T9617 |
14553-14560 |
JJ |
denotes |
Osmotic |
T9618 |
14561-14571 |
NN |
denotes |
resistance |
T9619 |
14572-14578 |
NNS |
denotes |
curves |
T9620 |
14579-14581 |
IN |
denotes |
of |
T9621 |
14582-14585 |
JJ |
denotes |
red |
T9622 |
14592-14597 |
NNS |
denotes |
cells |
T9623 |
14586-14591 |
NN |
denotes |
blood |
T9624 |
14598-14602 |
IN |
denotes |
from |
T9625 |
14603-14607 |
JJ |
denotes |
wild |
T9626 |
14608-14612 |
NN |
denotes |
type |
T9627 |
14633-14637 |
NNS |
denotes |
mice |
T9628 |
14613-14616 |
CC |
denotes |
and |
T9629 |
14617-14622 |
NN |
denotes |
anxA7 |
T9630 |
14622-14623 |
SYM |
denotes |
- |
T9631 |
14623-14624 |
HYPH |
denotes |
/ |
T9632 |
14624-14625 |
SYM |
denotes |
- |
T9633 |
14626-14632 |
JJ |
denotes |
mutant |
T9634 |
14637-14638 |
. |
denotes |
. |
T9635 |
14638-14781 |
sentence |
denotes |
The osmotic resistance towards changes in the extracellular ionic strength is a convenient assay for analysis of the red blood cell integrity. |
T9636 |
14639-14642 |
DT |
denotes |
The |
T9637 |
14651-14661 |
NN |
denotes |
resistance |
T9638 |
14643-14650 |
JJ |
denotes |
osmotic |
T9639 |
14714-14716 |
VBZ |
denotes |
is |
T9640 |
14662-14669 |
IN |
denotes |
towards |
T9641 |
14670-14677 |
NNS |
denotes |
changes |
T9642 |
14678-14680 |
IN |
denotes |
in |
T9643 |
14681-14684 |
DT |
denotes |
the |
T9644 |
14705-14713 |
NN |
denotes |
strength |
T9645 |
14685-14698 |
JJ |
denotes |
extracellular |
T9646 |
14699-14704 |
JJ |
denotes |
ionic |
T9647 |
14717-14718 |
DT |
denotes |
a |
T9648 |
14730-14735 |
NN |
denotes |
assay |
T9649 |
14719-14729 |
JJ |
denotes |
convenient |
T9650 |
14736-14739 |
IN |
denotes |
for |
T9651 |
14740-14748 |
NN |
denotes |
analysis |
T9652 |
14749-14751 |
IN |
denotes |
of |
T9653 |
14752-14755 |
DT |
denotes |
the |
T9654 |
14771-14780 |
NN |
denotes |
integrity |
T9655 |
14756-14759 |
JJ |
denotes |
red |
T9656 |
14766-14770 |
NN |
denotes |
cell |
T9657 |
14760-14765 |
NN |
denotes |
blood |
T9658 |
14780-14781 |
. |
denotes |
. |
T9659 |
14781-14948 |
sentence |
denotes |
It is measured as a sodium chloride concentration in which cellular lysis starts (minimal resistance) up to a complete lysis (maximal resistance) of a red blood cell. |
T9660 |
14782-14784 |
PRP |
denotes |
It |
T9661 |
14788-14796 |
VBN |
denotes |
measured |
T9662 |
14785-14787 |
VBZ |
denotes |
is |
T9663 |
14797-14799 |
IN |
denotes |
as |
T9664 |
14800-14801 |
DT |
denotes |
a |
T9665 |
14818-14831 |
NN |
denotes |
concentration |
T9666 |
14802-14808 |
NN |
denotes |
sodium |
T9667 |
14809-14817 |
NN |
denotes |
chloride |
T9668 |
14832-14834 |
IN |
denotes |
in |
T9669 |
14856-14862 |
VBZ |
denotes |
starts |
T9670 |
14835-14840 |
WDT |
denotes |
which |
T9671 |
14841-14849 |
JJ |
denotes |
cellular |
T9672 |
14850-14855 |
NN |
denotes |
lysis |
T9673 |
14863-14864 |
-LRB- |
denotes |
( |
T9674 |
14872-14882 |
NN |
denotes |
resistance |
T9675 |
14864-14871 |
JJ |
denotes |
minimal |
T9676 |
14882-14883 |
-RRB- |
denotes |
) |
T9677 |
14884-14886 |
IN |
denotes |
up |
T9678 |
14887-14889 |
IN |
denotes |
to |
T9679 |
14890-14891 |
DT |
denotes |
a |
T9680 |
14901-14906 |
NN |
denotes |
lysis |
T9681 |
14892-14900 |
JJ |
denotes |
complete |
T9682 |
14907-14908 |
-LRB- |
denotes |
( |
T9683 |
14916-14926 |
NN |
denotes |
resistance |
T9684 |
14908-14915 |
JJ |
denotes |
maximal |
T9685 |
14926-14927 |
-RRB- |
denotes |
) |
T9686 |
14928-14930 |
IN |
denotes |
of |
T9687 |
14931-14932 |
DT |
denotes |
a |
T9688 |
14943-14947 |
NN |
denotes |
cell |
T9689 |
14933-14936 |
JJ |
denotes |
red |
T9690 |
14937-14942 |
NN |
denotes |
blood |
T9691 |
14947-14948 |
. |
denotes |
. |
T9692 |
14948-15093 |
sentence |
denotes |
The osmotic resistance of annexin A7 deficient red blood cells is significantly increased compared to the one of wild type (p = 0.00066; n = 8). |
T9693 |
14949-14952 |
DT |
denotes |
The |
T9694 |
14961-14971 |
NN |
denotes |
resistance |
T9695 |
14953-14960 |
JJ |
denotes |
osmotic |
T9696 |
15012-15014 |
VBZ |
denotes |
is |
T9697 |
14972-14974 |
IN |
denotes |
of |
T9698 |
14975-14982 |
NN |
denotes |
annexin |
T9699 |
14983-14985 |
NN |
denotes |
A7 |
T9700 |
14986-14995 |
JJ |
denotes |
deficient |
T9701 |
15006-15011 |
NNS |
denotes |
cells |
T9702 |
14996-14999 |
JJ |
denotes |
red |
T9703 |
15000-15005 |
NN |
denotes |
blood |
T9704 |
15015-15028 |
RB |
denotes |
significantly |
T9705 |
15029-15038 |
JJ |
denotes |
increased |
T9706 |
15039-15047 |
VBN |
denotes |
compared |
T9707 |
15048-15050 |
IN |
denotes |
to |
T9708 |
15051-15054 |
DT |
denotes |
the |
T9709 |
15055-15058 |
CD |
denotes |
one |
T9710 |
15059-15061 |
IN |
denotes |
of |
T9711 |
15062-15066 |
JJ |
denotes |
wild |
T9712 |
15067-15071 |
NN |
denotes |
type |
T9713 |
15072-15073 |
-LRB- |
denotes |
( |
T9714 |
15090-15091 |
CD |
denotes |
8 |
T9715 |
15073-15074 |
NN |
denotes |
p |
T9716 |
15077-15084 |
CD |
denotes |
0.00066 |
T9717 |
15075-15076 |
SYM |
denotes |
= |
T9718 |
15084-15085 |
: |
denotes |
; |
T9719 |
15086-15087 |
NN |
denotes |
n |
T9720 |
15088-15089 |
SYM |
denotes |
= |
T9721 |
15091-15092 |
-RRB- |
denotes |
) |
T9722 |
15092-15093 |
. |
denotes |
. |
T9783 |
17790-17798 |
NN |
denotes |
Platelet |
T9784 |
17799-17810 |
NN |
denotes |
aggregation |
T9785 |
17811-17817 |
NNS |
denotes |
curves |
T9786 |
17818-17822 |
IN |
denotes |
from |
T9787 |
17823-17831 |
NN |
denotes |
platelet |
T9788 |
17832-17836 |
JJ |
denotes |
rich |
T9789 |
17837-17843 |
NN |
denotes |
plasma |
T9790 |
17844-17846 |
IN |
denotes |
of |
T9791 |
17847-17854 |
NN |
denotes |
annexin |
T9792 |
17855-17857 |
NN |
denotes |
A7 |
T9793 |
17882-17886 |
NNS |
denotes |
mice |
T9794 |
17858-17863 |
NN |
denotes |
knock |
T9795 |
17864-17867 |
NN |
denotes |
out |
T9796 |
17868-17871 |
CC |
denotes |
and |
T9797 |
17872-17876 |
JJ |
denotes |
wild |
T9798 |
17877-17881 |
NN |
denotes |
type |
T9799 |
17886-17887 |
. |
denotes |
. |
T9800 |
17887-18002 |
sentence |
denotes |
The transmission values were measured with an APACT photometer and aggregation was initiated by adding ristocetin. |
T9801 |
17888-17891 |
DT |
denotes |
The |
T9802 |
17905-17911 |
NNS |
denotes |
values |
T9803 |
17892-17904 |
NN |
denotes |
transmission |
T9804 |
17917-17925 |
VBN |
denotes |
measured |
T9805 |
17912-17916 |
VBD |
denotes |
were |
T9806 |
17926-17930 |
IN |
denotes |
with |
T9807 |
17931-17933 |
DT |
denotes |
an |
T9808 |
17940-17950 |
NN |
denotes |
photometer |
T9809 |
17934-17939 |
NN |
denotes |
APACT |
T9810 |
17951-17954 |
CC |
denotes |
and |
T9811 |
17955-17966 |
NN |
denotes |
aggregation |
T9812 |
17971-17980 |
VBN |
denotes |
initiated |
T9813 |
17967-17970 |
VBD |
denotes |
was |
T9814 |
17981-17983 |
IN |
denotes |
by |
T9815 |
17984-17990 |
VBG |
denotes |
adding |
T9816 |
17991-18001 |
NN |
denotes |
ristocetin |
T9817 |
18001-18002 |
. |
denotes |
. |
T9818 |
18002-18038 |
sentence |
denotes |
The first thirty seconds are given. |
T9819 |
18003-18006 |
DT |
denotes |
The |
T9820 |
18020-18027 |
NNS |
denotes |
seconds |
T9821 |
18007-18012 |
JJ |
denotes |
first |
T9822 |
18013-18019 |
CD |
denotes |
thirty |
T9823 |
18032-18037 |
VBN |
denotes |
given |
T9824 |
18028-18031 |
VBP |
denotes |
are |
T9825 |
18037-18038 |
. |
denotes |
. |
T9826 |
18038-18101 |
sentence |
denotes |
Constant platelet counts were used throughout all experiments. |
T9827 |
18039-18047 |
JJ |
denotes |
Constant |
T9828 |
18057-18063 |
NNS |
denotes |
counts |
T9829 |
18048-18056 |
NN |
denotes |
platelet |
T9830 |
18069-18073 |
VBN |
denotes |
used |
T9831 |
18064-18068 |
VBD |
denotes |
were |
T9832 |
18074-18084 |
IN |
denotes |
throughout |
T9833 |
18085-18088 |
DT |
denotes |
all |
T9834 |
18089-18100 |
NNS |
denotes |
experiments |
T9835 |
18100-18101 |
. |
denotes |
. |
T9836 |
18101-18234 |
sentence |
denotes |
The aggregation curve data were analysed for slope values, the maximal aggregation amplitude of every single curve was set to 100 %. |
T9837 |
18102-18105 |
DT |
denotes |
The |
T9838 |
18124-18128 |
NNS |
denotes |
data |
T9839 |
18106-18117 |
NN |
denotes |
aggregation |
T9840 |
18118-18123 |
NN |
denotes |
curve |
T9841 |
18134-18142 |
VBN |
denotes |
analysed |
T9842 |
18129-18133 |
VBD |
denotes |
were |
T9843 |
18221-18224 |
VBN |
denotes |
set |
T9844 |
18143-18146 |
IN |
denotes |
for |
T9845 |
18147-18152 |
NN |
denotes |
slope |
T9846 |
18153-18159 |
NNS |
denotes |
values |
T9847 |
18159-18161 |
, |
denotes |
, |
T9848 |
18161-18164 |
DT |
denotes |
the |
T9849 |
18185-18194 |
NN |
denotes |
amplitude |
T9850 |
18165-18172 |
JJ |
denotes |
maximal |
T9851 |
18173-18184 |
NN |
denotes |
aggregation |
T9852 |
18195-18197 |
IN |
denotes |
of |
T9853 |
18198-18203 |
DT |
denotes |
every |
T9854 |
18211-18216 |
NN |
denotes |
curve |
T9855 |
18204-18210 |
JJ |
denotes |
single |
T9856 |
18217-18220 |
VBD |
denotes |
was |
T9857 |
18225-18227 |
IN |
denotes |
to |
T9858 |
18228-18231 |
CD |
denotes |
100 |
T9859 |
18232-18233 |
NN |
denotes |
% |
T9860 |
18233-18234 |
. |
denotes |
. |
T9861 |
18234-18610 |
sentence |
denotes |
Both murine curves differ significantly at a time point of seven seconds after platelet initiation, the mutant shows a slightly lower aggregation velocity (p = 0.0287, n knock out = 16, n wild type = 15, mean knock out = 67.7 % transmission, mean wild type = 77.1 % transmission, standard error knock out = 8.3 % transmission, standard error wild type = 13.8 % transmission). |
T9862 |
18235-18239 |
DT |
denotes |
Both |
T9863 |
18247-18253 |
NNS |
denotes |
curves |
T9864 |
18240-18246 |
JJ |
denotes |
murine |
T9865 |
18254-18260 |
VBP |
denotes |
differ |
T9866 |
18346-18351 |
VBZ |
denotes |
shows |
T9867 |
18261-18274 |
RB |
denotes |
significantly |
T9868 |
18275-18277 |
IN |
denotes |
at |
T9869 |
18278-18279 |
DT |
denotes |
a |
T9870 |
18285-18290 |
NN |
denotes |
point |
T9871 |
18280-18284 |
NN |
denotes |
time |
T9872 |
18291-18293 |
IN |
denotes |
of |
T9873 |
18294-18299 |
CD |
denotes |
seven |
T9874 |
18300-18307 |
NNS |
denotes |
seconds |
T9875 |
18308-18313 |
IN |
denotes |
after |
T9876 |
18314-18322 |
NN |
denotes |
platelet |
T9877 |
18323-18333 |
NN |
denotes |
initiation |
T9878 |
18333-18335 |
, |
denotes |
, |
T9879 |
18335-18338 |
DT |
denotes |
the |
T9880 |
18339-18345 |
NN |
denotes |
mutant |
T9881 |
18352-18353 |
DT |
denotes |
a |
T9882 |
18381-18389 |
NN |
denotes |
velocity |
T9883 |
18354-18362 |
RB |
denotes |
slightly |
T9884 |
18363-18368 |
JJR |
denotes |
lower |
T9885 |
18369-18380 |
NN |
denotes |
aggregation |
T9886 |
18390-18391 |
-LRB- |
denotes |
( |
T9887 |
18596-18608 |
NN |
denotes |
transmission |
T9888 |
18391-18392 |
NN |
denotes |
p |
T9889 |
18395-18401 |
CD |
denotes |
0.0287 |
T9890 |
18393-18394 |
SYM |
denotes |
= |
T9891 |
18401-18403 |
, |
denotes |
, |
T9892 |
18403-18404 |
NN |
denotes |
n |
T9893 |
18411-18414 |
NN |
denotes |
out |
T9894 |
18405-18410 |
NN |
denotes |
knock |
T9895 |
18417-18419 |
CD |
denotes |
16 |
T9896 |
18415-18416 |
SYM |
denotes |
= |
T9897 |
18419-18421 |
, |
denotes |
, |
T9898 |
18421-18422 |
NN |
denotes |
n |
T9899 |
18428-18432 |
NN |
denotes |
type |
T9900 |
18423-18427 |
JJ |
denotes |
wild |
T9901 |
18435-18437 |
CD |
denotes |
15 |
T9902 |
18433-18434 |
SYM |
denotes |
= |
T9903 |
18437-18439 |
, |
denotes |
, |
T9904 |
18439-18443 |
JJ |
denotes |
mean |
T9905 |
18450-18453 |
NN |
denotes |
out |
T9906 |
18444-18449 |
NN |
denotes |
knock |
T9907 |
18463-18475 |
NN |
denotes |
transmission |
T9908 |
18454-18455 |
SYM |
denotes |
= |
T9909 |
18456-18460 |
CD |
denotes |
67.7 |
T9910 |
18461-18462 |
NN |
denotes |
% |
T9911 |
18475-18477 |
, |
denotes |
, |
T9912 |
18477-18481 |
JJ |
denotes |
mean |
T9913 |
18487-18491 |
NN |
denotes |
type |
T9914 |
18482-18486 |
JJ |
denotes |
wild |
T9915 |
18501-18513 |
NN |
denotes |
transmission |
T9916 |
18492-18493 |
SYM |
denotes |
= |
T9917 |
18494-18498 |
CD |
denotes |
77.1 |
T9918 |
18499-18500 |
NN |
denotes |
% |
T9919 |
18513-18515 |
, |
denotes |
, |
T9920 |
18515-18523 |
JJ |
denotes |
standard |
T9921 |
18524-18529 |
NN |
denotes |
error |
T9922 |
18536-18539 |
NN |
denotes |
out |
T9923 |
18530-18535 |
NN |
denotes |
knock |
T9924 |
18548-18560 |
NN |
denotes |
transmission |
T9925 |
18540-18541 |
SYM |
denotes |
= |
T9926 |
18542-18545 |
CD |
denotes |
8.3 |
T9927 |
18546-18547 |
NN |
denotes |
% |
T9928 |
18560-18562 |
, |
denotes |
, |
T9929 |
18562-18570 |
JJ |
denotes |
standard |
T9930 |
18571-18576 |
NN |
denotes |
error |
T9931 |
18582-18586 |
NN |
denotes |
type |
T9932 |
18577-18581 |
JJ |
denotes |
wild |
T9933 |
18587-18588 |
SYM |
denotes |
= |
T9934 |
18589-18593 |
CD |
denotes |
13.8 |
T9935 |
18594-18595 |
NN |
denotes |
% |
T9936 |
18608-18609 |
-RRB- |
denotes |
) |
T9937 |
18609-18610 |
. |
denotes |
. |
T9938 |
18610-18727 |
sentence |
denotes |
Murine platelets react immediately to the initiating chemical and show no lag phase like the normal human platelets. |
T9939 |
18611-18617 |
JJ |
denotes |
Murine |
T9940 |
18618-18627 |
NNS |
denotes |
platelets |
T9941 |
18628-18633 |
VBP |
denotes |
react |
T9942 |
18634-18645 |
RB |
denotes |
immediately |
T9943 |
18646-18648 |
IN |
denotes |
to |
T9944 |
18649-18652 |
DT |
denotes |
the |
T9945 |
18664-18672 |
NN |
denotes |
chemical |
T9946 |
18653-18663 |
VBG |
denotes |
initiating |
T9947 |
18673-18676 |
CC |
denotes |
and |
T9948 |
18677-18681 |
VBP |
denotes |
show |
T9949 |
18682-18684 |
DT |
denotes |
no |
T9950 |
18689-18694 |
NN |
denotes |
phase |
T9951 |
18685-18688 |
NN |
denotes |
lag |
T9952 |
18695-18699 |
IN |
denotes |
like |
T9953 |
18700-18703 |
DT |
denotes |
the |
T9954 |
18717-18726 |
NNS |
denotes |
platelets |
T9955 |
18704-18710 |
JJ |
denotes |
normal |
T9956 |
18711-18716 |
JJ |
denotes |
human |
T9957 |
18726-18727 |
. |
denotes |
. |
T9958 |
18727-18861 |
sentence |
denotes |
For comparison of the range of the platelet impairment slightly affected human platelets are shown as well (von Willebrand syndrome). |
T9959 |
18728-18731 |
IN |
denotes |
For |
T9960 |
18821-18826 |
VBN |
denotes |
shown |
T9961 |
18732-18742 |
NN |
denotes |
comparison |
T9962 |
18743-18745 |
IN |
denotes |
of |
T9963 |
18746-18749 |
DT |
denotes |
the |
T9964 |
18750-18755 |
NN |
denotes |
range |
T9965 |
18756-18758 |
IN |
denotes |
of |
T9966 |
18759-18762 |
DT |
denotes |
the |
T9967 |
18772-18782 |
NN |
denotes |
impairment |
T9968 |
18763-18771 |
NN |
denotes |
platelet |
T9969 |
18783-18791 |
RB |
denotes |
slightly |
T9970 |
18792-18800 |
VBN |
denotes |
affected |
T9971 |
18807-18816 |
NNS |
denotes |
platelets |
T9972 |
18801-18806 |
JJ |
denotes |
human |
T9973 |
18817-18820 |
VBP |
denotes |
are |
T9974 |
18827-18829 |
RB |
denotes |
as |
T9975 |
18830-18834 |
RB |
denotes |
well |
T9976 |
18835-18836 |
-LRB- |
denotes |
( |
T9977 |
18851-18859 |
NN |
denotes |
syndrome |
T9978 |
18836-18839 |
NNP |
denotes |
von |
T9979 |
18840-18850 |
NNP |
denotes |
Willebrand |
T9980 |
18859-18860 |
-RRB- |
denotes |
) |
T9981 |
18860-18861 |
. |
denotes |
. |
T2865 |
10303-10310 |
NN |
denotes |
annexin |
T2866 |
10311-10313 |
NN |
denotes |
A7 |
T2867 |
10314-10316 |
IN |
denotes |
in |
T2868 |
10317-10320 |
JJ |
denotes |
red |
T2869 |
10327-10332 |
NNS |
denotes |
cells |
T9489 |
12236-12240 |
JJ |
denotes |
Dark |
T9490 |
12241-12246 |
NN |
denotes |
field |
T9491 |
12247-12257 |
NN |
denotes |
microscopy |
T9492 |
12258-12260 |
IN |
denotes |
of |
T9493 |
12261-12264 |
JJ |
denotes |
red |
T9494 |
12271-12276 |
NNS |
denotes |
cells |
T9495 |
12265-12270 |
NN |
denotes |
blood |
T9496 |
12277-12281 |
IN |
denotes |
from |
T9497 |
12282-12287 |
JJ |
denotes |
anxA7 |
T9498 |
12291-12297 |
NN |
denotes |
mutant |
T9499 |
12287-12288 |
SYM |
denotes |
- |
T9500 |
12288-12289 |
HYPH |
denotes |
/ |
T9501 |
12289-12290 |
SYM |
denotes |
- |
T9502 |
12316-12320 |
NNS |
denotes |
mice |
T9503 |
12298-12299 |
-LRB- |
denotes |
( |
T9504 |
12299-12300 |
NN |
denotes |
B |
T9505 |
12300-12301 |
-RRB- |
denotes |
) |
T9506 |
12302-12305 |
CC |
denotes |
and |
T9507 |
12306-12310 |
JJ |
denotes |
wild |
T9508 |
12311-12315 |
NN |
denotes |
type |
T9509 |
12321-12322 |
-LRB- |
denotes |
( |
T9510 |
12322-12323 |
NN |
denotes |
A |
T9511 |
12323-12324 |
-RRB- |
denotes |
) |
T9512 |
12324-12325 |
. |
denotes |
. |
T9513 |
12325-12390 |
sentence |
denotes |
The mean cellular diameters are 6.0 μm and 5.7 μm, respectively. |
T9514 |
12326-12329 |
DT |
denotes |
The |
T9515 |
12344-12353 |
NNS |
denotes |
diameters |
T9516 |
12330-12334 |
JJ |
denotes |
mean |
T9517 |
12335-12343 |
JJ |
denotes |
cellular |
T9518 |
12354-12357 |
VBP |
denotes |
are |
T9519 |
12358-12361 |
CD |
denotes |
6.0 |
T9520 |
12362-12364 |
NNS |
denotes |
μm |
T9521 |
12365-12368 |
CC |
denotes |
and |
T9522 |
12369-12372 |
CD |
denotes |
5.7 |
T9523 |
12373-12375 |
NNS |
denotes |
μm |
T9524 |
12375-12377 |
, |
denotes |
, |
T9525 |
12377-12389 |
RB |
denotes |
respectively |
T9526 |
12389-12390 |
. |
denotes |
. |
T9527 |
12390-12462 |
sentence |
denotes |
The size differences are statistically significant (p = 0.015; n = 40). |
T9528 |
12391-12394 |
DT |
denotes |
The |
T9529 |
12400-12411 |
NNS |
denotes |
differences |
T9530 |
12395-12399 |
NN |
denotes |
size |
T9531 |
12412-12415 |
VBP |
denotes |
are |
T9532 |
12416-12429 |
RB |
denotes |
statistically |
T2870 |
10321-10326 |
NN |
denotes |
blood |
T2871 |
10333-10335 |
PRP |
denotes |
we |
T2872 |
10341-10345 |
NN |
denotes |
self |
T2873 |
10346-10353 |
VBG |
denotes |
forming |
T205 |
0-8 |
NN |
denotes |
Function |
T2874 |
10372-10381 |
NNS |
denotes |
gradients |
T2554 |
7731-7738 |
NN |
denotes |
isoform |
T2555 |
7724-7726 |
CD |
denotes |
47 |
T2556 |
7727-7730 |
NN |
denotes |
kDa |
T2557 |
7743-7751 |
VBN |
denotes |
detected |
T2558 |
7739-7742 |
VBD |
denotes |
was |
T2559 |
7752-7754 |
IN |
denotes |
in |
T2560 |
7755-7761 |
NN |
denotes |
silver |
T2561 |
7762-7769 |
VBN |
denotes |
stained |
T2562 |
7770-7774 |
NNS |
denotes |
gels |
T2563 |
7775-7778 |
CC |
denotes |
and |
T2564 |
7779-7782 |
PRP$ |
denotes |
its |
T2565 |
7783-7791 |
NN |
denotes |
identity |
T2566 |
7808-7817 |
VBN |
denotes |
confirmed |
T2567 |
7792-7796 |
IN |
denotes |
with |
T2568 |
7797-7804 |
NN |
denotes |
annexin |
T2569 |
7805-7807 |
NN |
denotes |
A7 |
T2570 |
7818-7820 |
IN |
denotes |
by |
T2571 |
7821-7828 |
NN |
denotes |
peptide |
T2572 |
7834-7848 |
NN |
denotes |
fingerprinting |
T2573 |
7829-7833 |
NN |
denotes |
mass |
T2574 |
7849-7850 |
-LRB- |
denotes |
( |
T2575 |
7859-7864 |
VBN |
denotes |
shown |
T2576 |
7850-7854 |
NNS |
denotes |
data |
T2577 |
7855-7858 |
RB |
denotes |
not |
T2578 |
7864-7865 |
-RRB- |
denotes |
) |
T2579 |
7865-7866 |
. |
denotes |
. |
T2580 |
7866-7921 |
sentence |
denotes |
The 51 kDa isoform was only detected in western blots. |
T2581 |
7867-7870 |
DT |
denotes |
The |
T2582 |
7878-7885 |
NN |
denotes |
isoform |
T2583 |
7871-7873 |
CD |
denotes |
51 |
T2584 |
7874-7877 |
NN |
denotes |
kDa |
T2585 |
7895-7903 |
VBN |
denotes |
detected |
T2586 |
7886-7889 |
VBD |
denotes |
was |
T2587 |
7890-7894 |
RB |
denotes |
only |
T2588 |
7904-7906 |
IN |
denotes |
in |
T2589 |
7907-7914 |
JJ |
denotes |
western |
T2590 |
7915-7920 |
NNS |
denotes |
blots |
T2591 |
7920-7921 |
. |
denotes |
. |
T2592 |
7921-7922 |
sentence |
denotes |
|
T2593 |
8306-8463 |
sentence |
denotes |
Its presence in vesicles led to the suggestion, that, along with raft domains, annexin A7 plays a role in membrane organization and the vesiculation process. |
T2594 |
8306-8309 |
PRP$ |
denotes |
Its |
T2595 |
8310-8318 |
NN |
denotes |
presence |
T2596 |
8331-8334 |
VBD |
denotes |
led |
T2597 |
8319-8321 |
IN |
denotes |
in |
T2598 |
8322-8330 |
NNS |
denotes |
vesicles |
T2599 |
8335-8337 |
IN |
denotes |
to |
T2600 |
8338-8341 |
DT |
denotes |
the |
T2601 |
8342-8352 |
NN |
denotes |
suggestion |
T2602 |
8352-8354 |
, |
denotes |
, |
T2603 |
8354-8358 |
IN |
denotes |
that |
T2604 |
8396-8401 |
VBZ |
denotes |
plays |
T2605 |
8358-8360 |
, |
denotes |
, |
T2875 |
10354-10363 |
NN |
denotes |
iodixanol |
T2792 |
9315-9316 |
. |
denotes |
. |
T2793 |
9316-9480 |
sentence |
denotes |
It appears that annexin A7, although it has been described to fuse membranes, is not a key component in the process of the formation of red blood cell exovesicles. |
T2794 |
9317-9319 |
PRP |
denotes |
It |
T2795 |
9320-9327 |
VBZ |
denotes |
appears |
T2796 |
9328-9332 |
IN |
denotes |
that |
T2797 |
9395-9397 |
VBZ |
denotes |
is |
T2798 |
9333-9340 |
NN |
denotes |
annexin |
T2799 |
9341-9343 |
NN |
denotes |
A7 |
T2800 |
9343-9345 |
, |
denotes |
, |
T2801 |
9345-9353 |
IN |
denotes |
although |
T2802 |
9366-9375 |
VBN |
denotes |
described |
T2803 |
9354-9356 |
PRP |
denotes |
it |
T2804 |
9357-9360 |
VBZ |
denotes |
has |
T2805 |
9361-9365 |
VBN |
denotes |
been |
T2806 |
9376-9378 |
TO |
denotes |
to |
T2807 |
9379-9383 |
VB |
denotes |
fuse |
T2808 |
9384-9393 |
NNS |
denotes |
membranes |
T2809 |
9393-9395 |
, |
denotes |
, |
T2810 |
9398-9401 |
RB |
denotes |
not |
T2811 |
9402-9403 |
DT |
denotes |
a |
T2812 |
9408-9417 |
NN |
denotes |
component |
T2813 |
9404-9407 |
JJ |
denotes |
key |
T2814 |
9418-9420 |
IN |
denotes |
in |
T2815 |
9421-9424 |
DT |
denotes |
the |
T2816 |
9425-9432 |
NN |
denotes |
process |
T2817 |
9433-9435 |
IN |
denotes |
of |
T2818 |
9436-9439 |
DT |
denotes |
the |
T2819 |
9440-9449 |
NN |
denotes |
formation |
T2820 |
9450-9452 |
IN |
denotes |
of |
T2821 |
9453-9456 |
JJ |
denotes |
red |
T2822 |
9463-9467 |
NN |
denotes |
cell |
T2823 |
9457-9462 |
NN |
denotes |
blood |
T2824 |
9468-9479 |
NNS |
denotes |
exovesicles |
T2825 |
9479-9480 |
. |
denotes |
. |
T2826 |
9480-9637 |
sentence |
denotes |
When we probed for the presence of sorcin in wild type and mutant vesicles we found that the sorcin levels were reduced in the mutant nanovesicles (Fig. 2). |
T2827 |
9481-9485 |
WRB |
denotes |
When |
T2828 |
9489-9495 |
VBD |
denotes |
probed |
T2829 |
9486-9488 |
PRP |
denotes |
we |
T2830 |
9559-9564 |
VBD |
denotes |
found |
T2831 |
9496-9499 |
IN |
denotes |
for |
T2858 |
9637-10382 |
sentence |
denotes |
Figure 2 Enrichment of annexin A7 and sorcin in exovesicles derived from red blood cells. Vesicles from wild type (wt) and anxA7-/- mutant (ko) were generated with Ca2+/ionophore treatment, isolated by differential centrifugation and analysed by immunoblotting with mAb 203–217 and a sorcin polyclonal antibody. In general, annexin A7 and sorcin are more abundant in nanovesicles. The 51 kDa isoform of annexin A7 (arrows) is only observed in nanovesicles. Samples of both vesicles types were normalized according to their acetylcholine esterase activity. Human red blood cells (co) were used for control and normalized independently. To study the distribution of annexin A7 in red blood cells we used self forming iodixanol density gradients. |
T2859 |
10274-10276 |
TO |
denotes |
To |
T2860 |
10277-10282 |
VB |
denotes |
study |
T2861 |
10336-10340 |
VBD |
denotes |
used |
T2862 |
10283-10286 |
DT |
denotes |
the |
T2863 |
10287-10299 |
NN |
denotes |
distribution |
T2864 |
10300-10302 |
IN |
denotes |
of |
T2876 |
10364-10371 |
NN |
denotes |
density |
T2877 |
10381-10382 |
. |
denotes |
. |
T2878 |
10382-10526 |
sentence |
denotes |
Both isoforms were present in the soluble fraction as well as in the gradient fractions where they are assumed to be associated with membranes. |
T2879 |
10383-10387 |
DT |
denotes |
Both |
T3356 |
11866-11871 |
NN |
denotes |
shape |
T3357 |
11872-11875 |
CC |
denotes |
and |
T3358 |
11876-11884 |
NN |
denotes |
diameter |
T3359 |
11885-11887 |
IN |
denotes |
of |
T3360 |
11888-11891 |
DT |
denotes |
the |
T3361 |
11910-11915 |
NNS |
denotes |
cells |
T3362 |
11892-11897 |
JJ |
denotes |
anxA7 |
T3363 |
11897-11898 |
SYM |
denotes |
- |
T3364 |
11898-11899 |
HYPH |
denotes |
/ |
T3365 |
11899-11900 |
SYM |
denotes |
- |
T3366 |
11900-11903 |
JJ |
denotes |
red |
T3367 |
11904-11909 |
NN |
denotes |
blood |
T3368 |
11915-11916 |
. |
denotes |
. |
T3369 |
11916-12120 |
sentence |
denotes |
They had a statistically significant larger cell diameter of 6.0 μm compared to wild type with 5.7 μm (p = 0.015, n = 40), a remarkably lower emphasized central impression and a more flat shape (Fig. 4). |
T3370 |
11917-11921 |
PRP |
denotes |
They |
T3371 |
11922-11925 |
VBD |
denotes |
had |
T3372 |
11926-11927 |
DT |
denotes |
a |
T3373 |
11966-11974 |
NN |
denotes |
diameter |
T3374 |
11928-11941 |
RB |
denotes |
statistically |
T3375 |
11942-11953 |
JJ |
denotes |
significant |
T3376 |
11954-11960 |
JJR |
denotes |
larger |
T3377 |
11961-11965 |
NN |
denotes |
cell |
T3378 |
11975-11977 |
IN |
denotes |
of |
T3379 |
11978-11981 |
CD |
denotes |
6.0 |
T3380 |
11982-11984 |
NN |
denotes |
μm |
T3381 |
11985-11993 |
VBN |
denotes |
compared |
T3436 |
12474-12565 |
sentence |
denotes |
Shape and MCV of a red blood cell essentially influence its function and capillary passage. |
T3437 |
12474-12479 |
NN |
denotes |
Shape |
T3438 |
12520-12529 |
VB |
denotes |
influence |
T3439 |
12480-12483 |
CC |
denotes |
and |
T3440 |
12484-12487 |
NN |
denotes |
MCV |
T3441 |
12488-12490 |
IN |
denotes |
of |
T3442 |
12491-12492 |
DT |
denotes |
a |
T3443 |
12503-12507 |
NN |
denotes |
cell |
T3444 |
12493-12496 |
JJ |
denotes |
red |
T3445 |
12497-12502 |
NN |
denotes |
blood |
T3446 |
12508-12519 |
RB |
denotes |
essentially |
T3447 |
12530-12533 |
PRP$ |
denotes |
its |
T3448 |
12534-12542 |
NN |
denotes |
function |
T3449 |
12543-12546 |
CC |
denotes |
and |
T3450 |
12547-12556 |
JJ |
denotes |
capillary |
T3451 |
12557-12564 |
NN |
denotes |
passage |
T3452 |
12564-12565 |
. |
denotes |
. |
T3453 |
12565-12739 |
sentence |
denotes |
The typical biconcave form depends on various influences including the membrane lipid composition and the submembranous cytoskeleton [32] where annexin A7 might play a role. |
T3454 |
12566-12569 |
DT |
denotes |
The |
T3455 |
12588-12592 |
NN |
denotes |
form |
T3456 |
12570-12577 |
JJ |
denotes |
typical |
T3457 |
12578-12587 |
NN |
denotes |
biconcave |
T3458 |
12593-12600 |
VBZ |
denotes |
depends |
T3459 |
12601-12603 |
IN |
denotes |
on |
T3460 |
12604-12611 |
JJ |
denotes |
various |
T3461 |
12612-12622 |
NNS |
denotes |
influences |
T3462 |
12623-12632 |
VBG |
denotes |
including |
T3463 |
12633-12636 |
DT |
denotes |
the |
T3464 |
12652-12663 |
NN |
denotes |
composition |
T3465 |
12637-12645 |
NN |
denotes |
membrane |
T3466 |
12646-12651 |
NN |
denotes |
lipid |
T3467 |
12664-12667 |
CC |
denotes |
and |
T3468 |
12668-12671 |
DT |
denotes |
the |
T3469 |
12686-12698 |
NN |
denotes |
cytoskeleton |
T3470 |
12672-12685 |
JJ |
denotes |
submembranous |
T3471 |
12699-12700 |
-LRB- |
denotes |
[ |
T3472 |
12700-12702 |
CD |
denotes |
32 |
T3473 |
12702-12703 |
-RRB- |
denotes |
] |
T3474 |
12704-12709 |
WRB |
denotes |
where |
T3715 |
13927-13930 |
DT |
denotes |
the |
T3716 |
13955-13968 |
NN |
denotes |
deformability |
T3717 |
13931-13934 |
JJ |
denotes |
red |
T1134 |
1843-1850 |
NN |
denotes |
Annexin |
T6556 |
26275-26279 |
IN |
denotes |
like |
T6557 |
26280-26283 |
DT |
denotes |
the |
T6558 |
26319-26330 |
NNS |
denotes |
differences |
T6559 |
26284-26292 |
NN |
denotes |
platelet |
T6560 |
26293-26300 |
NN |
denotes |
antigen |
T6561 |
26301-26313 |
NN |
denotes |
polymorphism |
T6562 |
26314-26315 |
-LRB- |
denotes |
[ |
T6563 |
26315-26317 |
CD |
denotes |
53 |
T6564 |
26317-26318 |
-RRB- |
denotes |
] |
T6565 |
26331-26336 |
MD |
denotes |
might |
T6566 |
26344-26352 |
JJ |
denotes |
apparent |
T6567 |
26352-26353 |
. |
denotes |
. |
R10 |
T217 |
T216 |
cc |
and,platelets |
R11 |
T218 |
T219 |
amod |
red,cells |
R12 |
T219 |
T216 |
conj |
cells,platelets |
R1 |
T208 |
T205 |
punct |
", ",Function |
R3 |
T210 |
T209 |
cc |
and,expression |
R4 |
T211 |
T209 |
conj |
localization,expression |
R5 |
T212 |
T205 |
prep |
of,Function |
R6 |
T213 |
T214 |
compound |
annexin,A7 |
R7 |
T214 |
T212 |
pobj |
A7,of |
R8 |
T215 |
T205 |
prep |
in,Function |
R9 |
T216 |
T215 |
pobj |
platelets,in |
R14 |
T221 |
T206 |
punct |
: ,Insights |
R15 |
T222 |
T206 |
acl |
derived,Insights |
R16 |
T223 |
T222 |
prep |
from,derived |
R17 |
T224 |
T225 |
det |
an,mouse |
R18 |
T225 |
T223 |
pobj |
mouse,from |
R22 |
T233 |
T234 |
compound |
Annexin,A7 |
R23 |
T234 |
T235 |
nsubj |
A7,is |
R24 |
T236 |
T237 |
det |
a,protein |
R25 |
T237 |
T235 |
attr |
protein,is |
R27 |
T239 |
T237 |
amod |
binding,protein |
R28 |
T240 |
T238 |
punct |
-,Ca2+ |
R29 |
T241 |
T238 |
cc |
and,Ca2+ |
R30 |
T242 |
T238 |
conj |
phospholipid,Ca2+ |
R31 |
T243 |
T239 |
punct |
-,binding |
R32 |
T244 |
T237 |
acl |
expressed,protein |
R33 |
T245 |
T244 |
prep |
as,expressed |
R34 |
T246 |
T247 |
det |
a,isoform |
R35 |
T247 |
T245 |
pobj |
isoform,as |
R37 |
T249 |
T247 |
compound |
kDa,isoform |
R38 |
T250 |
T248 |
cc |
and,47 |
R39 |
T251 |
T248 |
conj |
51,47 |
R40 |
T252 |
T247 |
punct |
", ",isoform |
R41 |
T253 |
T254 |
dep |
which,thought |
R42 |
T254 |
T247 |
relcl |
thought,isoform |
R44 |
T256 |
T257 |
aux |
to,involved |
R45 |
T257 |
T254 |
xcomp |
involved,thought |
R47 |
T259 |
T257 |
prep |
in,involved |
R48 |
T260 |
T261 |
compound |
membrane,fusion |
R49 |
T261 |
T262 |
compound |
fusion,processes |
R50 |
T262 |
T259 |
pobj |
processes,in |
R51 |
T263 |
T235 |
punct |
.,is |
R52 |
T265 |
T266 |
advmod |
Recently,identified |
R53 |
T267 |
T268 |
det |
the,isoform |
R54 |
T268 |
T266 |
nsubjpass |
isoform,identified |
R57 |
T271 |
T266 |
aux |
has,identified |
R58 |
T272 |
T266 |
auxpass |
been,identified |
R59 |
T273 |
T266 |
prep |
in,identified |
R60 |
T274 |
T273 |
pobj |
erythrocytes,in |
R61 |
T275 |
T276 |
advmod |
where,proposed |
R62 |
T276 |
T274 |
relcl |
proposed,erythrocytes |
R65 |
T279 |
T280 |
aux |
to,be |
R66 |
T280 |
T276 |
xcomp |
be,proposed |
R67 |
T281 |
T282 |
det |
a,component |
R68 |
T282 |
T280 |
attr |
component,be |
R70 |
T284 |
T282 |
prep |
in,component |
R71 |
T285 |
T286 |
det |
the,process |
R72 |
T286 |
T284 |
pobj |
process,in |
R73 |
T287 |
T286 |
prep |
of,process |
R74 |
T288 |
T289 |
det |
the,release |
R75 |
T289 |
T287 |
pobj |
release,of |
R77 |
T291 |
T289 |
amod |
dependent,release |
R78 |
T292 |
T291 |
punct |
-,dependent |
R80 |
T294 |
T286 |
punct |
", ",process |
R81 |
T295 |
T296 |
det |
a,process |
R82 |
T296 |
T286 |
appos |
process,process |
R83 |
T297 |
T298 |
prep |
with,protect |
R84 |
T298 |
T296 |
relcl |
protect,process |
R87 |
T301 |
T298 |
nsubj |
cells,protect |
R88 |
T302 |
T301 |
compound |
blood,cells |
R90 |
T304 |
T298 |
dobj |
themselves,protect |
R91 |
T305 |
T298 |
prep |
against,protect |
R92 |
T306 |
T307 |
det |
an,attack |
R93 |
T307 |
T305 |
pobj |
attack,against |
R94 |
T308 |
T307 |
prep |
by,attack |
R95 |
T309 |
T310 |
prep |
for,components |
R96 |
T310 |
T308 |
pobj |
components,by |
R99 |
T313 |
T266 |
punct |
.,identified |
R100 |
T317 |
T318 |
det |
The,role |
R101 |
T318 |
T319 |
nsubjpass |
role,addressed |
R102 |
T320 |
T318 |
prep |
of,role |
R103 |
T321 |
T322 |
compound |
annexin,A7 |
R104 |
T322 |
T320 |
pobj |
A7,of |
R105 |
T323 |
T318 |
prep |
in,role |
R106 |
T324 |
T325 |
amod |
red,cells |
R107 |
T325 |
T323 |
pobj |
cells,in |
R109 |
T327 |
T319 |
auxpass |
was,addressed |
R110 |
T328 |
T319 |
prep |
in,addressed |
R111 |
T329 |
T328 |
pobj |
erythrocytes,in |
R112 |
T330 |
T329 |
prep |
from,erythrocytes |
R113 |
T331 |
T332 |
nmod |
anxA7,mice |
R114 |
T332 |
T330 |
pobj |
mice,from |
R118 |
T336 |
T319 |
punct |
.,addressed |
R119 |
T338 |
T339 |
advmod |
Interestingly,impaired |
R120 |
T340 |
T339 |
punct |
", ",impaired |
R121 |
T341 |
T342 |
det |
the,process |
R122 |
T342 |
T339 |
nsubjpass |
process,impaired |
R124 |
T344 |
T342 |
amod |
mediated,process |
R125 |
T345 |
T344 |
punct |
-,mediated |
R127 |
T347 |
T339 |
auxpass |
was,impaired |
R128 |
T348 |
T339 |
neg |
not,impaired |
R129 |
T349 |
T339 |
punct |
.,impaired |
R130 |
T351 |
T352 |
advmod |
Also,appeared |
R131 |
T353 |
T352 |
punct |
", ",appeared |
R132 |
T354 |
T355 |
det |
the,organization |
R133 |
T355 |
T352 |
nsubj |
organization,appeared |
R135 |
T357 |
T358 |
neg |
not,disturbed |
R136 |
T358 |
T352 |
xcomp |
disturbed,appeared |
R139 |
T361 |
T362 |
mark |
as,assessed |
R140 |
T362 |
T358 |
advcl |
assessed,disturbed |
R141 |
T363 |
T362 |
advcl |
using,assessed |
R142 |
T364 |
T365 |
compound |
gradient,fractionation |
R143 |
T365 |
T366 |
compound |
fractionation,studies |
R144 |
T366 |
T363 |
dobj |
studies,using |
R145 |
T367 |
T352 |
punct |
.,appeared |
R146 |
T369 |
T370 |
advmod |
Instead,led |
R147 |
T371 |
T370 |
punct |
", ",led |
R148 |
T372 |
T370 |
nsubj |
lack,led |
R149 |
T373 |
T372 |
prep |
of,lack |
R150 |
T374 |
T375 |
compound |
annexin,A7 |
R151 |
T375 |
T373 |
pobj |
A7,of |
R152 |
T376 |
T370 |
prep |
to,led |
R153 |
T377 |
T378 |
det |
an,shape |
R154 |
T378 |
T376 |
pobj |
shape,to |
R157 |
T381 |
T378 |
cc |
and,shape |
R158 |
T382 |
T383 |
amod |
increased,resistance |
R159 |
T383 |
T378 |
conj |
resistance,shape |
R161 |
T385 |
T378 |
prep |
of,shape |
R162 |
T386 |
T387 |
amod |
red,cells |
R163 |
T387 |
T385 |
pobj |
cells,of |
R165 |
T389 |
T370 |
punct |
.,led |
R166 |
T391 |
T392 |
compound |
Annexin,A7 |
R167 |
T392 |
T393 |
nsubjpass |
A7,identified |
R168 |
T394 |
T393 |
auxpass |
was,identified |
R169 |
T395 |
T393 |
advmod |
also,identified |
R170 |
T396 |
T393 |
prep |
in,identified |
R171 |
T397 |
T396 |
pobj |
platelets,in |
R172 |
T398 |
T393 |
punct |
.,identified |
R173 |
T400 |
T401 |
prep |
In,led |
R174 |
T402 |
T403 |
det |
these,cells |
R175 |
T403 |
T400 |
pobj |
cells,In |
R176 |
T404 |
T405 |
poss |
its,loss |
R177 |
T405 |
T401 |
nsubj |
loss,led |
R178 |
T406 |
T401 |
prep |
to,led |
R179 |
T407 |
T408 |
det |
a,velocity |
R180 |
T408 |
T406 |
pobj |
velocity,to |
R184 |
T412 |
T413 |
dep |
which,seems |
R185 |
T413 |
T408 |
relcl |
seems,velocity |
R186 |
T414 |
T415 |
aux |
to,compensated |
R187 |
T415 |
T413 |
xcomp |
compensated,seems |
R189 |
T417 |
T415 |
prep |
by,compensated |
R190 |
T418 |
T419 |
det |
an,number |
R191 |
T419 |
T417 |
pobj |
number,by |
R193 |
T421 |
T419 |
prep |
of,number |
R194 |
T422 |
T421 |
pobj |
platelets,of |
R195 |
T423 |
T401 |
punct |
.,led |
R196 |
T425 |
T426 |
det |
The,results |
R197 |
T426 |
T427 |
nsubj |
results,appear |
R198 |
T428 |
T429 |
aux |
to,rule |
R199 |
T429 |
T427 |
xcomp |
rule,appear |
R200 |
T430 |
T429 |
prt |
out,rule |
R201 |
T431 |
T432 |
det |
an,role |
R202 |
T432 |
T429 |
dobj |
role,rule |
R204 |
T434 |
T432 |
prep |
of,role |
R205 |
T435 |
T436 |
compound |
annexin,A7 |
R206 |
T436 |
T434 |
pobj |
A7,of |
R207 |
T437 |
T432 |
prep |
in,role |
R208 |
T438 |
T439 |
compound |
membrane,fusion |
R209 |
T439 |
T440 |
compound |
fusion,processes |
R210 |
T440 |
T437 |
pobj |
processes,in |
R211 |
T441 |
T440 |
acl |
occurring,processes |
R212 |
T442 |
T441 |
prep |
in,occurring |
R213 |
T443 |
T444 |
amod |
red,cells |
R214 |
T444 |
T442 |
pobj |
cells,in |
R216 |
T446 |
T427 |
punct |
.,appear |
R217 |
T448 |
T449 |
advmod |
Instead,involved |
R218 |
T450 |
T451 |
det |
the,protein |
R219 |
T451 |
T449 |
nsubjpass |
protein,involved |
R220 |
T452 |
T449 |
aux |
might,involved |
R221 |
T453 |
T449 |
auxpass |
be,involved |
R222 |
T454 |
T449 |
prep |
in,involved |
R223 |
T455 |
T456 |
det |
the,organization |
R224 |
T456 |
T454 |
pobj |
organization,in |
R225 |
T457 |
T456 |
prep |
of,organization |
R226 |
T458 |
T459 |
det |
the,cytoskeleton |
R227 |
T459 |
T457 |
pobj |
cytoskeleton,of |
R229 |
T461 |
T449 |
punct |
.,involved |
R230 |
T463 |
T464 |
amod |
Red,cells |
R231 |
T464 |
T466 |
nsubj |
cells,represent |
R233 |
T467 |
T466 |
aux |
may,represent |
R234 |
T468 |
T469 |
det |
an,model |
R235 |
T469 |
T466 |
dobj |
model,represent |
R237 |
T471 |
T472 |
aux |
to,study |
R238 |
T472 |
T469 |
advcl |
study,model |
R239 |
T473 |
T474 |
det |
the,role |
R240 |
T474 |
T472 |
dobj |
role,study |
R241 |
T475 |
T474 |
prep |
of,role |
R242 |
T476 |
T477 |
compound |
annexin,A7 |
R243 |
T477 |
T475 |
pobj |
A7,of |
R244 |
T478 |
T474 |
prep |
in,role |
R245 |
T479 |
T480 |
amod |
cellular,processes |
R246 |
T480 |
T478 |
pobj |
processes,in |
R247 |
T481 |
T466 |
punct |
.,represent |
R248 |
T485 |
T486 |
nsubj |
We,demonstrated |
R249 |
T487 |
T486 |
aux |
have,demonstrated |
R250 |
T488 |
T489 |
det |
the,presence |
R251 |
T489 |
T486 |
dobj |
presence,demonstrated |
R252 |
T490 |
T489 |
prep |
of,presence |
R253 |
T491 |
T492 |
preconj |
both,isoforms |
R254 |
T492 |
T490 |
pobj |
isoforms,of |
R257 |
T495 |
T489 |
prep |
in,presence |
R258 |
T496 |
T497 |
amod |
red,cells |
R259 |
T497 |
T495 |
pobj |
cells,in |
R261 |
T499 |
T489 |
cc |
and,presence |
R262 |
T500 |
T501 |
det |
the,presence |
R263 |
T501 |
T489 |
conj |
presence,presence |
R264 |
T502 |
T501 |
prep |
of,presence |
R265 |
T503 |
T504 |
det |
the,isoform |
R266 |
T504 |
T502 |
pobj |
isoform,of |
R268 |
T506 |
T501 |
prep |
in,presence |
R269 |
T507 |
T506 |
pobj |
platelets,in |
R270 |
T508 |
T486 |
punct |
.,demonstrated |
R271 |
T510 |
T511 |
prep |
In,impairs |
R272 |
T512 |
T513 |
det |
both,types |
R273 |
T513 |
T510 |
pobj |
types,In |
R275 |
T515 |
T516 |
det |
the,loss |
R276 |
T516 |
T511 |
nsubj |
loss,impairs |
R277 |
T517 |
T516 |
prep |
of,loss |
R278 |
T518 |
T519 |
compound |
annexin,A7 |
R279 |
T519 |
T517 |
pobj |
A7,of |
R280 |
T520 |
T521 |
amod |
cellular,functions |
R281 |
T521 |
T511 |
dobj |
functions,impairs |
R282 |
T522 |
T511 |
punct |
.,impairs |
R283 |
T524 |
T525 |
det |
The,defects |
R284 |
T525 |
T526 |
nsubj |
defects,are |
R285 |
T527 |
T525 |
acl |
observed,defects |
R286 |
T528 |
T526 |
advmod |
however,are |
R287 |
T529 |
T526 |
neg |
not,are |
R288 |
T530 |
T526 |
acomp |
compatible,are |
R289 |
T531 |
T530 |
prep |
with,compatible |
R290 |
T532 |
T533 |
det |
a,role |
R291 |
T533 |
T531 |
pobj |
role,with |
R293 |
T535 |
T533 |
prep |
for,role |
R294 |
T536 |
T537 |
compound |
annexin,A7 |
R295 |
T537 |
T535 |
pobj |
A7,for |
R296 |
T538 |
T533 |
prep |
in,role |
R297 |
T539 |
T540 |
compound |
membrane,fusion |
R298 |
T540 |
T541 |
compound |
fusion,processes |
R299 |
T541 |
T538 |
pobj |
processes,in |
R300 |
T542 |
T533 |
prep |
in,role |
R301 |
T543 |
T544 |
det |
these,types |
R302 |
T544 |
T542 |
pobj |
types,in |
R304 |
T546 |
T526 |
punct |
.,are |
R305 |
T205 |
T206 |
dep |
Function,Insights |
R314 |
T1134 |
T1135 |
compound |
Annexin,A7 |
R315 |
T1135 |
T1136 |
nsubj |
A7,is |
R316 |
T1137 |
T1138 |
det |
a,protein |
R317 |
T1138 |
T1136 |
attr |
protein,is |
R318 |
T1139 |
T1140 |
npadvmod |
Ca2+,binding |
R319 |
T1140 |
T1138 |
amod |
binding,protein |
R320 |
T1141 |
T1139 |
punct |
-,Ca2+ |
R321 |
T1142 |
T1139 |
cc |
and,Ca2+ |
R322 |
T1143 |
T1139 |
conj |
phospholipid,Ca2+ |
R323 |
T1144 |
T1140 |
punct |
-,binding |
R324 |
T1145 |
T1138 |
punct |
", ",protein |
R325 |
T1146 |
T1147 |
dep |
which,isolated |
R326 |
T1147 |
T1138 |
relcl |
isolated,protein |
R327 |
T1148 |
T1147 |
auxpass |
was,isolated |
R328 |
T1149 |
T1147 |
prep |
as,isolated |
R329 |
T1150 |
T1151 |
det |
the,agent |
R330 |
T1151 |
T1149 |
pobj |
agent,as |
R331 |
T1152 |
T1153 |
dep |
that,mediated |
R332 |
T1153 |
T1151 |
relcl |
mediated,agent |
R333 |
T1154 |
T1153 |
dobj |
aggregation,mediated |
R334 |
T1155 |
T1154 |
prep |
of,aggregation |
R335 |
T1156 |
T1157 |
compound |
chromaffin,granules |
R336 |
T1157 |
T1155 |
pobj |
granules,of |
R337 |
T1158 |
T1154 |
cc |
and,aggregation |
R338 |
T1159 |
T1154 |
conj |
fusion,aggregation |
R339 |
T1160 |
T1159 |
prep |
of,fusion |
R340 |
T1161 |
T1162 |
compound |
phospholipid,membranes |
R341 |
T1162 |
T1160 |
pobj |
membranes,of |
R342 |
T1163 |
T1154 |
prep |
in,aggregation |
R343 |
T1164 |
T1165 |
det |
the,presence |
R344 |
T1165 |
T1163 |
pobj |
presence,in |
R345 |
T1166 |
T1165 |
prep |
of,presence |
R346 |
T1167 |
T1166 |
pobj |
Ca2+,of |
R347 |
T1168 |
T1136 |
punct |
.,is |
R348 |
T1170 |
T1171 |
det |
This,activity |
R349 |
T1171 |
T1172 |
nsubj |
activity,led |
R350 |
T1173 |
T1172 |
prep |
to,led |
R351 |
T1174 |
T1175 |
det |
the,proposal |
R352 |
T1175 |
T1173 |
pobj |
proposal,to |
R353 |
T1176 |
T1175 |
prep |
of,proposal |
R354 |
T1177 |
T1178 |
compound |
annexin,A7 |
R355 |
T1178 |
T1179 |
poss |
A7,involvement |
R356 |
T1179 |
T1176 |
pobj |
involvement,of |
R357 |
T1180 |
T1178 |
case |
's,A7 |
R358 |
T1181 |
T1179 |
prep |
in,involvement |
R359 |
T1182 |
T1183 |
det |
the,secretion |
R360 |
T1183 |
T1181 |
pobj |
secretion,in |
R361 |
T1184 |
T1183 |
amod |
exocytotic,secretion |
R362 |
T1185 |
T1183 |
prep |
of,secretion |
R363 |
T1186 |
T1185 |
pobj |
catecholamines,of |
R364 |
T1187 |
T1188 |
punct |
[,1 |
R365 |
T1188 |
T1172 |
parataxis |
1,led |
R366 |
T1189 |
T1188 |
punct |
],1 |
R367 |
T1190 |
T1172 |
punct |
.,led |
R368 |
T1192 |
T1193 |
det |
The,protein |
R369 |
T1193 |
T1194 |
nsubj |
protein,belongs |
R370 |
T1195 |
T1194 |
prep |
to,belongs |
R371 |
T1196 |
T1197 |
det |
a,family |
R372 |
T1197 |
T1195 |
pobj |
family,to |
R373 |
T1198 |
T1197 |
prep |
of,family |
R374 |
T1199 |
T1200 |
advmod |
evolutionarily,conserved |
R375 |
T1200 |
T1201 |
amod |
conserved,proteins |
R376 |
T1201 |
T1198 |
pobj |
proteins,of |
R377 |
T1202 |
T1201 |
prep |
of,proteins |
R378 |
T1203 |
T1204 |
det |
a,structure |
R379 |
T1204 |
T1202 |
pobj |
structure,of |
R380 |
T1205 |
T1204 |
amod |
bipartite,structure |
R381 |
T1206 |
T1201 |
prep |
with,proteins |
R382 |
T1207 |
T1208 |
det |
a,variable |
R383 |
T1208 |
T1206 |
pobj |
variable,with |
R384 |
T1209 |
T1210 |
npadvmod |
N,terminal |
R385 |
T1210 |
T1208 |
amod |
terminal,variable |
R386 |
T1211 |
T1210 |
punct |
-,terminal |
R387 |
T1212 |
T1208 |
cc |
and,variable |
R388 |
T1213 |
T1214 |
det |
a,conserved |
R389 |
T1214 |
T1208 |
conj |
conserved,variable |
R390 |
T1215 |
T1216 |
npadvmod |
C,terminal |
R391 |
T1216 |
T1214 |
amod |
terminal,conserved |
R392 |
T1217 |
T1216 |
punct |
-,terminal |
R393 |
T1218 |
T1208 |
appos |
domain,variable |
R394 |
T1219 |
T1194 |
punct |
.,belongs |
R395 |
T1221 |
T1222 |
det |
The,domain |
R396 |
T1222 |
T1226 |
nsubj |
domain,is |
R397 |
T1223 |
T1224 |
npadvmod |
C,terminal |
R398 |
T1224 |
T1222 |
amod |
terminal,domain |
R399 |
T1225 |
T1224 |
punct |
-,terminal |
R400 |
T1226 |
T1227 |
ccomp |
is,appears |
R401 |
T1228 |
T1226 |
acomp |
responsible,is |
R402 |
T1229 |
T1228 |
prep |
for,responsible |
R403 |
T1230 |
T1231 |
det |
the,Ca2+ |
R404 |
T1231 |
T1229 |
pobj |
Ca2+,for |
R405 |
T1232 |
T1231 |
punct |
-,Ca2+ |
R406 |
T1233 |
T1231 |
cc |
and,Ca2+ |
R407 |
T1234 |
T1231 |
conj |
phospholipid,Ca2+ |
R408 |
T1235 |
T1231 |
punct |
-,Ca2+ |
R409 |
T1236 |
T1231 |
amod |
binding,Ca2+ |
R410 |
T1237 |
T1227 |
punct |
", ",appears |
R411 |
T1238 |
T1239 |
det |
the,domain |
R412 |
T1239 |
T1227 |
nsubj |
domain,appears |
R413 |
T1240 |
T1241 |
npadvmod |
N,terminal |
R414 |
T1241 |
T1239 |
amod |
terminal,domain |
R415 |
T1242 |
T1241 |
punct |
-,terminal |
R416 |
T1243 |
T1244 |
aux |
to,confer |
R417 |
T1244 |
T1227 |
xcomp |
confer,appears |
R418 |
T1245 |
T1246 |
amod |
functional,diversity |
R419 |
T1246 |
T1244 |
dobj |
diversity,confer |
R420 |
T1247 |
T1248 |
punct |
[,2 |
R421 |
T1248 |
T1227 |
parataxis |
2,appears |
R422 |
T1249 |
T1250 |
punct |
-,4 |
R423 |
T1250 |
T1248 |
prep |
4,2 |
R424 |
T1251 |
T1248 |
punct |
],2 |
R425 |
T1252 |
T1227 |
punct |
.,appears |
R426 |
T1254 |
T1255 |
det |
The,binding |
R427 |
T1255 |
T1256 |
nsubjpass |
binding,thought |
R428 |
T1257 |
T1255 |
prep |
to,binding |
R429 |
T1258 |
T1259 |
advmod |
negatively,charged |
R430 |
T1259 |
T1260 |
amod |
charged,phospholipids |
R431 |
T1260 |
T1257 |
pobj |
phospholipids,to |
R432 |
T1261 |
T1256 |
auxpass |
is,thought |
R433 |
T1262 |
T1263 |
aux |
to,mediated |
R434 |
T1263 |
T1256 |
xcomp |
mediated,thought |
R435 |
T1264 |
T1263 |
auxpass |
be,mediated |
R436 |
T1265 |
T1263 |
agent |
by,mediated |
R437 |
T1266 |
T1267 |
compound |
Ca2+,ions |
R438 |
T1267 |
T1265 |
pobj |
ions,by |
R439 |
T1268 |
T1256 |
punct |
.,thought |
R440 |
T1270 |
T1271 |
compound |
Annexin,A7 |
R441 |
T1271 |
T1272 |
nsubj |
A7,is |
R442 |
T1273 |
T1272 |
acomp |
unique,is |
R443 |
T1274 |
T1275 |
mark |
in,carries |
R444 |
T1275 |
T1272 |
advcl |
carries,is |
R445 |
T1276 |
T1275 |
mark |
that,carries |
R446 |
T1277 |
T1275 |
nsubj |
it,carries |
R447 |
T1278 |
T1279 |
det |
an,terminus |
R448 |
T1279 |
T1275 |
dobj |
terminus,carries |
R449 |
T1280 |
T1281 |
advmod |
extraordinarily,long |
R450 |
T1281 |
T1279 |
amod |
long,terminus |
R451 |
T1282 |
T1281 |
cc |
and,long |
R452 |
T1283 |
T1281 |
conj |
hydrophobic,long |
R453 |
T1284 |
T1279 |
compound |
amino,terminus |
R454 |
T1285 |
T1279 |
prep |
with,terminus |
R455 |
T1286 |
T1287 |
amod |
more,100 |
R456 |
T1287 |
T1289 |
nummod |
100,acids |
R457 |
T1288 |
T1287 |
quantmod |
than,100 |
R458 |
T1289 |
T1285 |
pobj |
acids,with |
R459 |
T1290 |
T1289 |
compound |
amino,acids |
R460 |
T1291 |
T1272 |
punct |
.,is |
R461 |
T1293 |
T1294 |
amod |
Alternative,splicing |
R462 |
T1294 |
T1295 |
nsubj |
splicing,gives |
R463 |
T1296 |
T1295 |
dobj |
rise,gives |
R464 |
T1297 |
T1295 |
prep |
to,gives |
R465 |
T1298 |
T1299 |
nummod |
two,isoforms |
R466 |
T1299 |
T1297 |
pobj |
isoforms,to |
R467 |
T1300 |
T1299 |
prep |
of,isoforms |
R468 |
T1301 |
T1302 |
nummod |
47,kDa |
R469 |
T1302 |
T1300 |
pobj |
kDa,of |
R470 |
T1303 |
T1301 |
cc |
and,47 |
R471 |
T1304 |
T1301 |
conj |
51,47 |
R472 |
T1305 |
T1295 |
punct |
.,gives |
R473 |
T1307 |
T1308 |
det |
Both,isoforms |
R474 |
T1308 |
T1309 |
nsubj |
isoforms,differ |
R475 |
T1310 |
T1309 |
prep |
by,differ |
R476 |
T1311 |
T1312 |
det |
an,exon |
R477 |
T1312 |
T1310 |
pobj |
exon,by |
R478 |
T1313 |
T1312 |
amod |
additional,exon |
R479 |
T1314 |
T1312 |
compound |
cassette,exon |
R480 |
T1315 |
T1312 |
amod |
located,exon |
R481 |
T1316 |
T1315 |
prep |
in,located |
R482 |
T1317 |
T1318 |
det |
the,third |
R483 |
T1318 |
T1316 |
pobj |
third,in |
R484 |
T1319 |
T1318 |
amod |
first,third |
R485 |
T1320 |
T1318 |
prep |
of,third |
R486 |
T1321 |
T1322 |
det |
the,domain |
R487 |
T1322 |
T1320 |
pobj |
domain,of |
R488 |
T1323 |
T1324 |
npadvmod |
N,terminal |
R489 |
T1324 |
T1322 |
amod |
terminal,domain |
R490 |
T1325 |
T1324 |
punct |
-,terminal |
R491 |
T1326 |
T1309 |
punct |
.,differ |
R492 |
T1328 |
T1329 |
amod |
Most,tissues |
R493 |
T1329 |
T1330 |
nsubj |
tissues,harbor |
R494 |
T1330 |
T1331 |
ccomp |
harbor,found |
R495 |
T1332 |
T1333 |
advmod |
only,isoform |
R496 |
T1333 |
T1330 |
dobj |
isoform,harbor |
R497 |
T1334 |
T1333 |
det |
the,isoform |
R498 |
T1335 |
T1336 |
nummod |
47,kDa |
R499 |
T1336 |
T1333 |
compound |
kDa,isoform |
R500 |
T1337 |
T1331 |
punct |
", ",found |
R501 |
T1338 |
T1339 |
det |
both,forms |
R502 |
T1339 |
T1331 |
nsubjpass |
forms,found |
R503 |
T1340 |
T1331 |
auxpass |
are,found |
R504 |
T1341 |
T1331 |
prep |
in,found |
R505 |
T1342 |
T1341 |
pobj |
brain,in |
R506 |
T1343 |
T1342 |
cc |
and,brain |
R507 |
T1344 |
T1342 |
conj |
heart,brain |
R508 |
T1345 |
T1331 |
punct |
", ",found |
R509 |
T1346 |
T1347 |
prep |
while,expressed |
R510 |
T1347 |
T1331 |
advcl |
expressed,found |
R511 |
T1348 |
T1349 |
det |
the,isoform |
R512 |
T1349 |
T1347 |
nsubjpass |
isoform,expressed |
R513 |
T1350 |
T1349 |
amod |
large,isoform |
R514 |
T1351 |
T1347 |
auxpass |
is,expressed |
R515 |
T1352 |
T1347 |
advmod |
exclusively,expressed |
R516 |
T1353 |
T1347 |
prep |
in,expressed |
R517 |
T1354 |
T1355 |
amod |
mature,muscle |
R518 |
T1355 |
T1353 |
pobj |
muscle,in |
R519 |
T1356 |
T1355 |
amod |
skeletal,muscle |
R520 |
T1357 |
T1331 |
punct |
.,found |
R521 |
T1359 |
T1360 |
prep |
At,detected |
R522 |
T1361 |
T1362 |
det |
the,level |
R523 |
T1362 |
T1359 |
pobj |
level,At |
R524 |
T1363 |
T1362 |
amod |
cellular,level |
R525 |
T1364 |
T1365 |
compound |
annexin,A7 |
R526 |
T1365 |
T1360 |
nsubjpass |
A7,detected |
R527 |
T1366 |
T1360 |
aux |
can,detected |
R528 |
T1367 |
T1360 |
auxpass |
be,detected |
R529 |
T1368 |
T1360 |
prep |
in,detected |
R530 |
T1369 |
T1370 |
det |
the,cytosol |
R531 |
T1370 |
T1368 |
pobj |
cytosol,in |
R532 |
T1371 |
T1368 |
punct |
", ",in |
R533 |
T1372 |
T1368 |
conj |
at,in |
R534 |
T1373 |
T1374 |
det |
the,membrane |
R535 |
T1374 |
T1372 |
pobj |
membrane,at |
R536 |
T1375 |
T1374 |
compound |
plasma,membrane |
R537 |
T1376 |
T1372 |
punct |
", ",at |
R538 |
T1377 |
T1372 |
conj |
around,at |
R539 |
T1378 |
T1379 |
det |
the,nucleus |
R540 |
T1379 |
T1377 |
pobj |
nucleus,around |
R541 |
T1380 |
T1377 |
punct |
", ",around |
R542 |
T1381 |
T1377 |
conj |
at,around |
R543 |
T1382 |
T1383 |
amod |
vesicular,structures |
R544 |
T1383 |
T1381 |
pobj |
structures,at |
R545 |
T1384 |
T1383 |
prep |
including,structures |
R546 |
T1385 |
T1386 |
amod |
adrenal,granules |
R547 |
T1386 |
T1384 |
pobj |
granules,including |
R548 |
T1387 |
T1386 |
compound |
chromaffin,granules |
R549 |
T1388 |
T1381 |
punct |
", ",at |
R550 |
T1389 |
T1381 |
cc |
and,at |
R551 |
T1390 |
T1381 |
conj |
at,at |
R552 |
T1391 |
T1392 |
det |
the,system |
R553 |
T1392 |
T1390 |
pobj |
system,at |
R554 |
T1393 |
T1394 |
compound |
t,tubule |
R555 |
T1394 |
T1392 |
compound |
tubule,system |
R556 |
T1395 |
T1394 |
punct |
-,tubule |
R557 |
T1396 |
T1397 |
punct |
[,6 |
R558 |
T1397 |
T1360 |
parataxis |
6,detected |
R559 |
T1398 |
T1397 |
nummod |
5,6 |
R560 |
T1399 |
T1397 |
punct |
",",6 |
R561 |
T1400 |
T1397 |
punct |
],6 |
R562 |
T1401 |
T1360 |
punct |
.,detected |
R563 |
T1403 |
T1404 |
compound |
Annexin,A7 |
R564 |
T1404 |
T1405 |
nsubj |
A7,translocates |
R565 |
T1406 |
T1405 |
prep |
to,translocates |
R566 |
T1407 |
T1406 |
pobj |
membranes,to |
R567 |
T1408 |
T1405 |
prep |
in,translocates |
R568 |
T1409 |
T1410 |
det |
a,fashion |
R569 |
T1410 |
T1408 |
pobj |
fashion,in |
R570 |
T1411 |
T1412 |
npadvmod |
Ca2+,dependent |
R571 |
T1412 |
T1410 |
amod |
dependent,fashion |
R572 |
T1413 |
T1412 |
punct |
-,dependent |
R573 |
T1414 |
T1405 |
cc |
and,translocates |
R574 |
T1415 |
T1416 |
punct |
", ",redistributes |
R575 |
T1416 |
T1405 |
conj |
redistributes,translocates |
R576 |
T1417 |
T1418 |
advmod |
when,rise |
R577 |
T1418 |
T1416 |
advcl |
rise,redistributes |
R578 |
T1419 |
T1420 |
amod |
intracellular,levels |
R579 |
T1420 |
T1418 |
nsubj |
levels,rise |
R580 |
T1421 |
T1420 |
compound |
Ca2+,levels |
R581 |
T1422 |
T1416 |
punct |
", ",redistributes |
R582 |
T1423 |
T1416 |
advmod |
sequentially,redistributes |
R583 |
T1424 |
T1416 |
prep |
to,redistributes |
R584 |
T1425 |
T1426 |
det |
the,plasma |
R585 |
T1426 |
T1424 |
pobj |
plasma,to |
R586 |
T1427 |
T1426 |
cc |
and,plasma |
R587 |
T1428 |
T1429 |
det |
the,membrane |
R588 |
T1429 |
T1426 |
conj |
membrane,plasma |
R589 |
T1430 |
T1429 |
amod |
nuclear,membrane |
R590 |
T1431 |
T1432 |
advmod |
as,as |
R591 |
T1432 |
T1424 |
cc |
as,to |
R592 |
T1433 |
T1432 |
advmod |
well,as |
R593 |
T1434 |
T1424 |
conj |
to,to |
R594 |
T1435 |
T1436 |
amod |
intracellular,vesicles |
R595 |
T1436 |
T1434 |
pobj |
vesicles,to |
R596 |
T1437 |
T1405 |
punct |
.,translocates |
R597 |
T1439 |
T1440 |
advmod |
Furthermore,associates |
R598 |
T1441 |
T1440 |
punct |
", ",associates |
R599 |
T1442 |
T1443 |
compound |
annexin,A7 |
R600 |
T1443 |
T1440 |
nsubj |
A7,associates |
R601 |
T1444 |
T1440 |
prep |
with,associates |
R602 |
T1445 |
T1446 |
compound |
lipid,rafts |
R603 |
T1446 |
T1444 |
pobj |
rafts,with |
R604 |
T1447 |
T1448 |
punct |
[,7 |
R605 |
T1448 |
T1440 |
parataxis |
7,associates |
R606 |
T1449 |
T1448 |
punct |
],7 |
R607 |
T1450 |
T1440 |
punct |
.,associates |
R608 |
T1452 |
T1453 |
compound |
Lipid,rafts |
R609 |
T1453 |
T1454 |
nsubj |
rafts,play |
R610 |
T1455 |
T1456 |
det |
a,role |
R611 |
T1456 |
T1454 |
dobj |
role,play |
R612 |
T1457 |
T1456 |
amod |
key,role |
R613 |
T1458 |
T1454 |
prep |
in,play |
R614 |
T1459 |
T1460 |
compound |
membrane,budding |
R615 |
T1460 |
T1458 |
pobj |
budding,in |
R616 |
T1461 |
T1458 |
cc |
and,in |
R617 |
T1462 |
T1458 |
conj |
in,in |
R618 |
T1463 |
T1464 |
compound |
vesiculation,processes |
R619 |
T1464 |
T1462 |
pobj |
processes,in |
R620 |
T1465 |
T1466 |
amod |
such,as |
R621 |
T1466 |
T1464 |
prep |
as,processes |
R622 |
T1467 |
T1468 |
nmod |
endo,exocytosis |
R623 |
T1468 |
T1466 |
pobj |
exocytosis,as |
R624 |
T1469 |
T1468 |
punct |
-,exocytosis |
R625 |
T1470 |
T1468 |
cc |
and,exocytosis |
R626 |
T1471 |
T1472 |
punct |
[,8 |
R627 |
T1472 |
T1454 |
parataxis |
8,play |
R628 |
T1473 |
T1474 |
punct |
-,10 |
R629 |
T1474 |
T1472 |
prep |
10,8 |
R630 |
T1475 |
T1472 |
punct |
],8 |
R631 |
T1476 |
T1454 |
punct |
.,play |
R632 |
T1478 |
T1479 |
nummod |
Two,partners |
R633 |
T1479 |
T1481 |
nsubjpass |
partners,identified |
R634 |
T1480 |
T1479 |
amod |
binding,partners |
R635 |
T1482 |
T1479 |
prep |
of,partners |
R636 |
T1483 |
T1484 |
compound |
annexin,A7 |
R637 |
T1484 |
T1482 |
pobj |
A7,of |
R638 |
T1485 |
T1481 |
aux |
have,identified |
R639 |
T1486 |
T1481 |
auxpass |
been,identified |
R640 |
T1487 |
T1481 |
punct |
", ",identified |
R641 |
T1488 |
T1481 |
dobj |
sorcin,identified |
R642 |
T1489 |
T1488 |
cc |
and,sorcin |
R643 |
T1490 |
T1488 |
conj |
galectin,sorcin |
R644 |
T1491 |
T1490 |
punct |
-,galectin |
R645 |
T1492 |
T1490 |
nummod |
3,galectin |
R646 |
T1493 |
T1494 |
punct |
[,11 |
R647 |
T1494 |
T1481 |
parataxis |
11,identified |
R648 |
T1495 |
T1496 |
punct |
-,13 |
R649 |
T1496 |
T1494 |
prep |
13,11 |
R650 |
T1497 |
T1494 |
punct |
],11 |
R651 |
T1498 |
T1481 |
punct |
.,identified |
R652 |
T1500 |
T1501 |
nsubj |
Sorcin,is |
R653 |
T1502 |
T1503 |
det |
a,protein |
R654 |
T1503 |
T1501 |
attr |
protein,is |
R655 |
T1504 |
T1505 |
npadvmod |
Ca2+,binding |
R656 |
T1505 |
T1503 |
amod |
binding,protein |
R657 |
T1506 |
T1505 |
punct |
-,binding |
R658 |
T1507 |
T1501 |
cc |
and,is |
R659 |
T1508 |
T1501 |
conj |
belongs,is |
R660 |
T1509 |
T1508 |
prep |
to,belongs |
R661 |
T1510 |
T1511 |
det |
the,family |
R662 |
T1511 |
T1509 |
pobj |
family,to |
R663 |
T1512 |
T1513 |
compound |
penta,protein |
R664 |
T1513 |
T1511 |
compound |
protein,family |
R665 |
T1514 |
T1515 |
compound |
EF,hand |
R666 |
T1515 |
T1513 |
compound |
hand,protein |
R667 |
T1516 |
T1515 |
punct |
-,hand |
R668 |
T1517 |
T1518 |
punct |
[,14 |
R669 |
T1518 |
T1508 |
parataxis |
14,belongs |
R670 |
T1519 |
T1518 |
punct |
],14 |
R671 |
T1520 |
T1501 |
punct |
.,is |
R672 |
T1522 |
T1523 |
prep |
Like,binds |
R673 |
T1524 |
T1525 |
compound |
annexin,A7 |
R674 |
T1525 |
T1522 |
pobj |
A7,Like |
R675 |
T1526 |
T1523 |
nsubj |
it,binds |
R676 |
T1527 |
T1523 |
prep |
to,binds |
R677 |
T1528 |
T1527 |
pobj |
membranes,to |
R678 |
T1529 |
T1523 |
prep |
in,binds |
R679 |
T1530 |
T1531 |
det |
a,manner |
R680 |
T1531 |
T1529 |
pobj |
manner,in |
R681 |
T1532 |
T1533 |
npadvmod |
Ca2+,dependent |
R682 |
T1533 |
T1531 |
amod |
dependent,manner |
R683 |
T1534 |
T1533 |
punct |
-,dependent |
R684 |
T1535 |
T1523 |
punct |
.,binds |
R685 |
T1537 |
T1538 |
nsubjpass |
Sorcin,described |
R686 |
T1539 |
T1538 |
advmod |
also,described |
R687 |
T1540 |
T1538 |
aux |
has,described |
R688 |
T1541 |
T1538 |
auxpass |
been,described |
R689 |
T1542 |
T1538 |
prep |
as,described |
R690 |
T1543 |
T1544 |
compound |
interaction,partner |
R691 |
T1544 |
T1542 |
pobj |
partner,as |
R692 |
T1545 |
T1544 |
prep |
of,partner |
R693 |
T1546 |
T1547 |
det |
the,receptor |
R694 |
T1547 |
T1545 |
pobj |
receptor,of |
R695 |
T1548 |
T1547 |
compound |
ryanodine,receptor |
R696 |
T1549 |
T1538 |
punct |
", ",described |
R697 |
T1550 |
T1538 |
cc |
and,described |
R698 |
T1551 |
T1538 |
conj |
appears,described |
R699 |
T1552 |
T1553 |
aux |
to,modulate |
R700 |
T1553 |
T1551 |
xcomp |
modulate,appears |
R701 |
T1554 |
T1555 |
poss |
its,function |
R702 |
T1555 |
T1553 |
dobj |
function,modulate |
R703 |
T1556 |
T1557 |
punct |
[,15 |
R704 |
T1557 |
T1551 |
parataxis |
15,appears |
R705 |
T1558 |
T1557 |
punct |
],15 |
R706 |
T1559 |
T1538 |
punct |
.,described |
R707 |
T1561 |
T1562 |
det |
The,influence |
R708 |
T1562 |
T1563 |
nsubj |
influence,is |
R709 |
T1564 |
T1562 |
prep |
of,influence |
R710 |
T1565 |
T1566 |
det |
the,interaction |
R711 |
T1566 |
T1564 |
pobj |
interaction,of |
R712 |
T1567 |
T1568 |
compound |
sorcin,annexin |
R713 |
T1568 |
T1570 |
compound |
annexin,A7 |
R714 |
T1569 |
T1568 |
punct |
/,annexin |
R715 |
T1570 |
T1566 |
compound |
A7,interaction |
R716 |
T1571 |
T1562 |
prep |
on,influence |
R717 |
T1572 |
T1573 |
det |
the,receptor |
R718 |
T1573 |
T1571 |
pobj |
receptor,on |
R719 |
T1574 |
T1573 |
compound |
ryanodine,receptor |
R720 |
T1575 |
T1563 |
acomp |
unknown,is |
R721 |
T1576 |
T1563 |
punct |
.,is |
R722 |
T1578 |
T1579 |
nsubjpass |
Sorcin,coexpressed |
R723 |
T1580 |
T1578 |
cc |
and,Sorcin |
R724 |
T1581 |
T1582 |
compound |
annexin,A7 |
R725 |
T1582 |
T1578 |
conj |
A7,Sorcin |
R726 |
T1583 |
T1579 |
auxpass |
are,coexpressed |
R727 |
T1584 |
T1579 |
prep |
in,coexpressed |
R728 |
T1585 |
T1586 |
det |
all,tissues |
R729 |
T1586 |
T1584 |
pobj |
tissues,in |
R730 |
T1587 |
T1586 |
acl |
examined,tissues |
R731 |
T1588 |
T1589 |
advmod |
so,far |
R732 |
T1589 |
T1587 |
advmod |
far,examined |
R733 |
T1590 |
T1591 |
punct |
[,11 |
R734 |
T1591 |
T1579 |
parataxis |
11,coexpressed |
R735 |
T1592 |
T1591 |
punct |
],11 |
R736 |
T1593 |
T1579 |
punct |
.,coexpressed |
R737 |
T1595 |
T1596 |
det |
The,binding |
R738 |
T1596 |
T1597 |
nsubj |
binding,is |
R739 |
T1598 |
T1596 |
prep |
of,binding |
R740 |
T1599 |
T1600 |
compound |
annexin,A7 |
R741 |
T1600 |
T1598 |
pobj |
A7,of |
R742 |
T1601 |
T1600 |
cc |
and,A7 |
R743 |
T1602 |
T1600 |
conj |
sorcin,A7 |
R744 |
T1603 |
T1604 |
npadvmod |
Ca2+,dependent |
R745 |
T1604 |
T1597 |
acomp |
dependent,is |
R746 |
T1605 |
T1604 |
punct |
-,dependent |
R747 |
T1606 |
T1597 |
cc |
and,is |
R748 |
T1607 |
T1597 |
conj |
occurs,is |
R749 |
T1608 |
T1607 |
prep |
at,occurs |
R750 |
T1609 |
T1610 |
amod |
micromolar,Ca2+ |
R751 |
T1610 |
T1611 |
compound |
Ca2+,concentrations |
R752 |
T1611 |
T1608 |
pobj |
concentrations,at |
R753 |
T1612 |
T1597 |
punct |
.,is |
R754 |
T1614 |
T1615 |
det |
The,sites |
R755 |
T1615 |
T1617 |
nsubjpass |
sites,localised |
R756 |
T1616 |
T1615 |
amod |
binding,sites |
R757 |
T1618 |
T1617 |
aux |
have,localised |
R758 |
T1619 |
T1617 |
auxpass |
been,localised |
R759 |
T1620 |
T1617 |
prep |
to,localised |
R760 |
T1621 |
T1622 |
det |
the,motifs |
R761 |
T1622 |
T1620 |
pobj |
motifs,to |
R762 |
T1623 |
T1624 |
npadvmod |
amino,terminal |
R763 |
T1624 |
T1622 |
amod |
terminal,motifs |
R764 |
T1625 |
T1622 |
nmod |
GGYY,motifs |
R765 |
T1626 |
T1625 |
cc |
and,GGYY |
R766 |
T1627 |
T1625 |
conj |
GYGG,GGYY |
R767 |
T1628 |
T1622 |
prep |
in,motifs |
R768 |
T1629 |
T1628 |
pobj |
sorcin,in |
R769 |
T1630 |
T1620 |
cc |
and,to |
R770 |
T1631 |
T1620 |
conj |
to,to |
R771 |
T1632 |
T1633 |
det |
the,motif |
R772 |
T1633 |
T1631 |
pobj |
motif,to |
R773 |
T1634 |
T1633 |
compound |
GYPP,motif |
R774 |
T1635 |
T1633 |
prep |
in,motif |
R775 |
T1636 |
T1637 |
det |
the,terminus |
R776 |
T1637 |
T1635 |
pobj |
terminus,in |
R777 |
T1638 |
T1637 |
compound |
amino,terminus |
R778 |
T1639 |
T1637 |
prep |
of,terminus |
R779 |
T1640 |
T1641 |
compound |
annexin,A7 |
R780 |
T1641 |
T1639 |
pobj |
A7,of |
R781 |
T1642 |
T1617 |
punct |
.,localised |
R782 |
T1644 |
T1645 |
det |
The,proteins |
R783 |
T1645 |
T1646 |
nsubj |
proteins,bind |
R784 |
T1647 |
T1646 |
prep |
to,bind |
R785 |
T1648 |
T1649 |
det |
each,other |
R786 |
T1649 |
T1647 |
pobj |
other,to |
R787 |
T1650 |
T1646 |
prep |
with,bind |
R788 |
T1651 |
T1652 |
det |
a,stoichiometry |
R789 |
T1652 |
T1650 |
pobj |
stoichiometry,with |
R790 |
T1653 |
T1652 |
prep |
of,stoichiometry |
R791 |
T1654 |
T1655 |
nummod |
two,molecules |
R792 |
T1655 |
T1653 |
pobj |
molecules,of |
R793 |
T1656 |
T1655 |
compound |
sorcin,molecules |
R794 |
T1657 |
T1655 |
prep |
per,molecules |
R795 |
T1658 |
T1659 |
compound |
annexin,A7 |
R796 |
T1659 |
T1660 |
compound |
A7,molecule |
R797 |
T1660 |
T1657 |
pobj |
molecule,per |
R798 |
T1661 |
T1662 |
punct |
[,12 |
R799 |
T1662 |
T1646 |
parataxis |
12,bind |
R800 |
T1663 |
T1662 |
punct |
],12 |
R801 |
T1664 |
T1646 |
punct |
.,bind |
R802 |
T1666 |
T1667 |
nsubj |
Galectin,is |
R803 |
T1668 |
T1666 |
punct |
-,Galectin |
R804 |
T1669 |
T1666 |
nummod |
3,Galectin |
R805 |
T1670 |
T1671 |
det |
a,protein |
R806 |
T1671 |
T1667 |
attr |
protein,is |
R807 |
T1672 |
T1671 |
amod |
multifunctional,protein |
R808 |
T1673 |
T1671 |
amod |
oncogenic,protein |
R809 |
T1674 |
T1671 |
prep |
with,protein |
R810 |
T1675 |
T1676 |
det |
an,activity |
R811 |
T1676 |
T1674 |
pobj |
activity,with |
R812 |
T1677 |
T1676 |
amod |
anti-apoptotic,activity |
R813 |
T1678 |
T1671 |
acl |
found,protein |
R814 |
T1679 |
T1678 |
prep |
in,found |
R815 |
T1680 |
T1681 |
det |
the,space |
R816 |
T1681 |
T1679 |
pobj |
space,in |
R817 |
T1682 |
T1681 |
amod |
extracellular,space |
R818 |
T1683 |
T1681 |
punct |
", ",space |
R819 |
T1684 |
T1681 |
prep |
in,space |
R820 |
T1685 |
T1686 |
det |
the,nucleus |
R821 |
T1686 |
T1684 |
pobj |
nucleus,in |
R822 |
T1687 |
T1686 |
cc |
and,nucleus |
R823 |
T1688 |
T1686 |
conj |
cytoplasm,nucleus |
R824 |
T1689 |
T1679 |
cc |
and,in |
R825 |
T1690 |
T1679 |
conj |
in,in |
R826 |
T1691 |
T1690 |
pobj |
mitochondria,in |
R827 |
T1692 |
T1667 |
punct |
.,is |
R828 |
T1694 |
T1695 |
amod |
Cytoplasmic,galectin |
R829 |
T1695 |
T1696 |
nsubj |
galectin,correlates |
R830 |
T1697 |
T1695 |
punct |
-,galectin |
R831 |
T1698 |
T1695 |
nummod |
3,galectin |
R832 |
T1699 |
T1696 |
prep |
with,correlates |
R833 |
T1700 |
T1701 |
compound |
tumor,progression |
R834 |
T1701 |
T1699 |
pobj |
progression,with |
R835 |
T1702 |
T1696 |
cc |
and,correlates |
R836 |
T1703 |
T1696 |
conj |
protects,correlates |
R837 |
T1704 |
T1705 |
amod |
mitochondrial,integrity |
R838 |
T1705 |
T1703 |
dobj |
integrity,protects |
R839 |
T1706 |
T1696 |
punct |
.,correlates |
R840 |
T1708 |
T1709 |
compound |
Down,regulation |
R841 |
T1709 |
T1710 |
nsubj |
regulation,prevents |
R842 |
T1711 |
T1709 |
prep |
of,regulation |
R843 |
T1712 |
T1713 |
compound |
annexin,A7 |
R844 |
T1713 |
T1711 |
pobj |
A7,of |
R845 |
T1714 |
T1715 |
compound |
galectin,3 |
R846 |
T1715 |
T1717 |
compound |
3,translocation |
R847 |
T1716 |
T1715 |
punct |
-,3 |
R848 |
T1717 |
T1710 |
dobj |
translocation,prevents |
R849 |
T1718 |
T1717 |
prep |
to,translocation |
R850 |
T1719 |
T1720 |
det |
the,membrane |
R851 |
T1720 |
T1718 |
pobj |
membrane,to |
R852 |
T1721 |
T1720 |
amod |
perinuclear,membrane |
R853 |
T1722 |
T1710 |
cc |
and,prevents |
R854 |
T1723 |
T1710 |
conj |
increases,prevents |
R855 |
T1724 |
T1725 |
compound |
galectin,3 |
R856 |
T1725 |
T1727 |
compound |
3,secretion |
R857 |
T1726 |
T1725 |
punct |
-,3 |
R858 |
T1727 |
T1723 |
dobj |
secretion,increases |
R859 |
T1728 |
T1710 |
punct |
.,prevents |
R860 |
T1730 |
T1731 |
prep |
For,proposed |
R861 |
T1732 |
T1733 |
compound |
annexin,A7 |
R862 |
T1733 |
T1730 |
pobj |
A7,For |
R863 |
T1734 |
T1735 |
det |
a,role |
R864 |
T1735 |
T1731 |
nsubjpass |
role,proposed |
R865 |
T1736 |
T1735 |
prep |
for,role |
R866 |
T1737 |
T1738 |
compound |
galectin,3 |
R867 |
T1738 |
T1740 |
compound |
3,trafficking |
R868 |
T1739 |
T1738 |
punct |
-,3 |
R869 |
T1740 |
T1736 |
pobj |
trafficking,for |
R870 |
T1741 |
T1740 |
punct |
", ",trafficking |
R871 |
T1742 |
T1743 |
compound |
apoptosis,regulation |
R872 |
T1743 |
T1740 |
conj |
regulation,trafficking |
R873 |
T1744 |
T1743 |
punct |
", ",regulation |
R874 |
T1745 |
T1743 |
cc |
and,regulation |
R875 |
T1746 |
T1747 |
amod |
mitochondrial,integrity |
R876 |
T1747 |
T1743 |
conj |
integrity,regulation |
R877 |
T1748 |
T1731 |
auxpass |
was,proposed |
R878 |
T1749 |
T1750 |
punct |
[,13 |
R879 |
T1750 |
T1731 |
parataxis |
13,proposed |
R880 |
T1751 |
T1750 |
punct |
],13 |
R881 |
T1752 |
T1731 |
punct |
.,proposed |
R882 |
T1754 |
T1755 |
det |
The,role |
R883 |
T1755 |
T1757 |
nsubjpass |
role,understood |
R884 |
T1756 |
T1755 |
amod |
cellular,role |
R885 |
T1758 |
T1755 |
prep |
of,role |
R886 |
T1759 |
T1760 |
compound |
annexin,A7 |
R887 |
T1760 |
T1758 |
pobj |
A7,of |
R888 |
T1761 |
T1757 |
auxpass |
is,understood |
R889 |
T1762 |
T1757 |
neg |
not,understood |
R890 |
T1763 |
T1757 |
advmod |
well,understood |
R891 |
T1764 |
T1757 |
punct |
.,understood |
R892 |
T1766 |
T1767 |
nsubjpass |
It,thought |
R893 |
T1768 |
T1767 |
auxpass |
is,thought |
R894 |
T1769 |
T1770 |
aux |
to,regulate |
R895 |
T1770 |
T1767 |
xcomp |
regulate,thought |
R896 |
T1771 |
T1770 |
cc |
and,regulate |
R897 |
T1772 |
T1770 |
conj |
stabilize,regulate |
R898 |
T1773 |
T1774 |
compound |
membrane,domains |
R899 |
T1774 |
T1772 |
dobj |
domains,stabilize |
R900 |
T1775 |
T1770 |
cc |
and,regulate |
R901 |
T1776 |
T1777 |
aux |
to,have |
R902 |
T1777 |
T1770 |
conj |
have,regulate |
R903 |
T1778 |
T1779 |
det |
a,role |
R904 |
T1779 |
T1777 |
dobj |
role,have |
R905 |
T1780 |
T1777 |
prep |
in,have |
R906 |
T1781 |
T1782 |
compound |
Ca2+,homeostasis |
R907 |
T1782 |
T1780 |
pobj |
homeostasis,in |
R908 |
T1783 |
T1782 |
cc |
and,homeostasis |
R909 |
T1784 |
T1785 |
npadvmod |
Ca2+,dependent |
R910 |
T1785 |
T1787 |
amod |
dependent,pathways |
R911 |
T1786 |
T1785 |
punct |
-,dependent |
R912 |
T1787 |
T1782 |
conj |
pathways,homeostasis |
R913 |
T1788 |
T1787 |
compound |
signaling,pathways |
R914 |
T1789 |
T1767 |
punct |
.,thought |
R915 |
T1791 |
T1792 |
det |
These,proposals |
R916 |
T1792 |
T1793 |
nsubjpass |
proposals,supported |
R917 |
T1794 |
T1793 |
auxpass |
are,supported |
R918 |
T1795 |
T1793 |
agent |
by,supported |
R919 |
T1796 |
T1795 |
pobj |
data,by |
R920 |
T1797 |
T1796 |
acl |
obtained,data |
R921 |
T1798 |
T1797 |
prep |
from,obtained |
R922 |
T1799 |
T1798 |
pobj |
analysis,from |
R923 |
T1800 |
T1799 |
prep |
of,analysis |
R924 |
T1801 |
T1802 |
compound |
annexin,A7 |
R925 |
T1802 |
T1803 |
npadvmod |
A7,deficient |
R926 |
T1803 |
T1804 |
amod |
deficient,mutants |
R927 |
T1804 |
T1800 |
pobj |
mutants,of |
R928 |
T1805 |
T1804 |
compound |
mouse,mutants |
R929 |
T1806 |
T1793 |
punct |
.,supported |
R930 |
T1808 |
T1809 |
nummod |
Two,strains |
R931 |
T1809 |
T1815 |
nsubjpass |
strains,generated |
R932 |
T1810 |
T1811 |
nmod |
annexin,A7 |
R933 |
T1811 |
T1809 |
nmod |
A7,strains |
R934 |
T1812 |
T1809 |
nmod |
129Sv,strains |
R935 |
T1919 |
T1917 |
cc |
and,cells |
R936 |
T1813 |
T1809 |
amod |
null,strains |
R937 |
T1920 |
T1917 |
conj |
platelets,cells |
R938 |
T1814 |
T1809 |
compound |
mice,strains |
R939 |
T1921 |
T1914 |
auxpass |
were,thought |
R940 |
T1816 |
T1815 |
auxpass |
were,generated |
R941 |
T1817 |
T1815 |
advmod |
independently,generated |
R942 |
T1922 |
T1914 |
neg |
not,thought |
R943 |
T1818 |
T1815 |
advcl |
using,generated |
R944 |
T1819 |
T1820 |
det |
a,strategy |
R945 |
T1923 |
T1924 |
aux |
to,contain |
R946 |
T1820 |
T1818 |
dobj |
strategy,using |
R947 |
T1821 |
T1820 |
amod |
different,strategy |
R948 |
T1924 |
T1914 |
xcomp |
contain,thought |
R949 |
T1822 |
T1815 |
punct |
.,generated |
R950 |
T1824 |
T1825 |
det |
The,one |
R951 |
T1925 |
T1926 |
compound |
annexin,A7 |
R952 |
T1825 |
T1826 |
nsubj |
one,is |
R953 |
T1926 |
T1924 |
dobj |
A7,contain |
R954 |
T1927 |
T1914 |
cc |
and,thought |
R955 |
T1928 |
T1929 |
advmod |
only,recently |
R956 |
T1827 |
T1825 |
prep |
of,one |
R957 |
T1929 |
T1930 |
advmod |
recently,reported |
R958 |
T1828 |
T1827 |
pobj |
Srivastava,of |
R959 |
T1829 |
T1830 |
advmod |
et,al. |
R960 |
T1830 |
T1828 |
advmod |
al.,Srivastava |
R961 |
T1930 |
T1914 |
conj |
reported,thought |
R962 |
T1831 |
T1832 |
punct |
[,16 |
R963 |
T1832 |
T1828 |
parataxis |
16,Srivastava |
R964 |
T1931 |
T1932 |
det |
the,isoform |
R965 |
T1833 |
T1832 |
punct |
],16 |
R966 |
T1834 |
T1826 |
acomp |
lethal,is |
R967 |
T1835 |
T1826 |
punct |
.,is |
R968 |
T1932 |
T1930 |
nsubjpass |
isoform,reported |
R969 |
T1837 |
T1838 |
amod |
Heterozygous,mice |
R970 |
T1933 |
T1934 |
nummod |
47,kDa |
R971 |
T1838 |
T1839 |
nsubj |
mice,exhibit |
R972 |
T1934 |
T1932 |
compound |
kDa,isoform |
R973 |
T1840 |
T1841 |
det |
an,defect |
R974 |
T1841 |
T1839 |
dobj |
defect,exhibit |
R975 |
T1842 |
T1843 |
compound |
insulin,secretion |
R976 |
T1935 |
T1930 |
aux |
has,reported |
R977 |
T1843 |
T1841 |
compound |
secretion,defect |
R978 |
T1844 |
T1841 |
cc |
and,defect |
R979 |
T1845 |
T1846 |
compound |
tumor,phenotypes |
R980 |
T1936 |
T1930 |
auxpass |
been,reported |
R981 |
T1846 |
T1841 |
conj |
phenotypes,defect |
R982 |
T1847 |
T1839 |
punct |
.,exhibit |
R983 |
T1937 |
T1930 |
prep |
as,reported |
R984 |
T1849 |
T1850 |
det |
The,strain |
R985 |
T1850 |
T1852 |
nsubj |
strain,is |
R986 |
T1851 |
T1850 |
amod |
null,strain |
R987 |
T1853 |
T1850 |
acl |
reported,strain |
R988 |
T1938 |
T1937 |
pobj |
component,as |
R989 |
T1854 |
T1853 |
agent |
by,reported |
R990 |
T1855 |
T1854 |
pobj |
Herr,by |
R991 |
T1939 |
T1938 |
prep |
of,component |
R992 |
T1856 |
T1857 |
advmod |
et,al. |
R993 |
T1857 |
T1855 |
advmod |
al.,Herr |
R994 |
T1858 |
T1859 |
punct |
[,17 |
R995 |
T1940 |
T1941 |
amod |
red,cells |
R996 |
T1859 |
T1853 |
parataxis |
17,reported |
R997 |
T1860 |
T1859 |
punct |
],17 |
R998 |
T1941 |
T1939 |
pobj |
cells,of |
R999 |
T1861 |
T1862 |
amod |
viable,healthy |
R1000 |
T1862 |
T1852 |
acomp |
healthy,is |
R1001 |
T1863 |
T1862 |
punct |
", ",healthy |
R1002 |
T1942 |
T1941 |
compound |
blood,cells |
R1003 |
T1864 |
T1852 |
punct |
", ",is |
R1004 |
T1865 |
T1852 |
cc |
and,is |
R1005 |
T1943 |
T1944 |
punct |
[,7 |
R1006 |
T1866 |
T1852 |
conj |
shows,is |
R1007 |
T1867 |
T1868 |
det |
no,defect |
R1008 |
T1868 |
T1866 |
dobj |
defect,shows |
R1009 |
T1944 |
T1930 |
parataxis |
7,reported |
R1010 |
T1869 |
T1870 |
compound |
insulin,secretion |
R1011 |
T1870 |
T1868 |
compound |
secretion,defect |
R1012 |
T1945 |
T1944 |
punct |
],7 |
R1013 |
T1871 |
T1868 |
cc |
or,defect |
R1014 |
T1946 |
T1914 |
punct |
.,thought |
R1015 |
T1872 |
T1873 |
amod |
other,defects |
R1016 |
T1948 |
T1949 |
amod |
Red,cells |
R1017 |
T1873 |
T1868 |
conj |
defects,defect |
R1018 |
T1874 |
T1873 |
amod |
obvious,defects |
R1019 |
T1875 |
T1852 |
punct |
.,is |
R1020 |
T1949 |
T1951 |
nsubj |
cells,undergo |
R1021 |
T1877 |
T1878 |
advmod |
However,disturbed |
R1022 |
T1950 |
T1949 |
compound |
blood,cells |
R1023 |
T1879 |
T1878 |
punct |
", ",disturbed |
R1024 |
T1880 |
T1878 |
prep |
in,disturbed |
R1025 |
T1952 |
T1953 |
amod |
various,changes |
R1026 |
T1881 |
T1882 |
amod |
isolated,cardiomyocytes |
R1027 |
T1882 |
T1880 |
pobj |
cardiomyocytes,in |
R1028 |
T1883 |
T1884 |
det |
the,shortening |
R1029 |
T1953 |
T1951 |
dobj |
changes,undergo |
R1030 |
T1884 |
T1878 |
nsubjpass |
shortening,disturbed |
R1031 |
T1885 |
T1886 |
npadvmod |
frequency,induced |
R1032 |
T1954 |
T1953 |
amod |
biochemical,changes |
R1033 |
T1886 |
T1884 |
amod |
induced,shortening |
R1034 |
T1887 |
T1886 |
punct |
-,induced |
R1035 |
T1888 |
T1878 |
auxpass |
is,disturbed |
R1036 |
T1889 |
T1878 |
punct |
.,disturbed |
R1037 |
T1955 |
T1954 |
cc |
or,biochemical |
R1038 |
T1891 |
T1892 |
advmod |
Here,focused |
R1039 |
T1956 |
T1954 |
conj |
morphological,biochemical |
R1040 |
T1893 |
T1892 |
nsubj |
we,focused |
R1041 |
T1957 |
T1958 |
dep |
that,appear |
R1042 |
T1894 |
T1892 |
aux |
have,focused |
R1043 |
T1895 |
T1892 |
prep |
on,focused |
R1044 |
T1896 |
T1897 |
det |
the,analysis |
R1045 |
T1958 |
T1953 |
relcl |
appear,changes |
R1046 |
T1897 |
T1895 |
pobj |
analysis,on |
R1047 |
T1898 |
T1897 |
prep |
of,analysis |
R1048 |
T1959 |
T1960 |
aux |
to,be |
R1049 |
T1899 |
T1900 |
amod |
red,cells |
R1050 |
T1900 |
T1898 |
pobj |
cells,of |
R1051 |
T1901 |
T1900 |
compound |
blood,cells |
R1052 |
T1960 |
T1958 |
xcomp |
be,appear |
R1053 |
T1902 |
T1900 |
cc |
and,cells |
R1054 |
T1903 |
T1900 |
conj |
platelets,cells |
R1055 |
T1904 |
T1900 |
prep |
of,cells |
R1056 |
T1961 |
T1962 |
npadvmod |
Ca2+,dependent |
R1057 |
T1905 |
T1906 |
det |
the,mutant |
R1058 |
T1906 |
T1904 |
pobj |
mutant,of |
R1059 |
T1907 |
T1906 |
nmod |
anxA7,mutant |
R1060 |
T1962 |
T1964 |
amod |
dependent,processes |
R1061 |
T1908 |
T1907 |
punct |
-,anxA7 |
R1062 |
T1909 |
T1907 |
punct |
/,anxA7 |
R1063 |
T1963 |
T1962 |
punct |
-,dependent |
R1064 |
T1910 |
T1907 |
punct |
-,anxA7 |
R1065 |
T1911 |
T1892 |
punct |
.,focused |
R1066 |
T1964 |
T1960 |
attr |
processes,be |
R1067 |
T1913 |
T1914 |
advmod |
Generally,thought |
R1068 |
T1915 |
T1914 |
punct |
", ",thought |
R1069 |
T1965 |
T1966 |
punct |
[,19 |
R1070 |
T1916 |
T1917 |
amod |
red,cells |
R1071 |
T1917 |
T1914 |
nsubjpass |
cells,thought |
R1072 |
T1966 |
T1951 |
parataxis |
19,undergo |
R1073 |
T1918 |
T1917 |
compound |
blood,cells |
R1074 |
T1967 |
T1966 |
nummod |
18,19 |
R1075 |
T1968 |
T1966 |
punct |
",",19 |
R1076 |
T1969 |
T1966 |
punct |
],19 |
R1077 |
T1970 |
T1951 |
punct |
.,undergo |
R1078 |
T1972 |
T1973 |
nsubj |
One,is |
R1079 |
T2026 |
T2027 |
dep |
which,lack |
R1080 |
T2027 |
T2023 |
relcl |
lack,cells |
R1081 |
T1974 |
T1972 |
prep |
of,One |
R1082 |
T2028 |
T2029 |
det |
the,ability |
R1083 |
T2029 |
T2027 |
dobj |
ability,lack |
R1084 |
T2030 |
T2031 |
aux |
to,vesiculate |
R1085 |
T1975 |
T1974 |
pobj |
them,of |
R1086 |
T2031 |
T2029 |
acl |
vesiculate,ability |
R1087 |
T2032 |
T2033 |
det |
a,disease |
R1088 |
T2033 |
T2025 |
dobj |
disease,cause |
R1089 |
T2034 |
T2025 |
prep |
with,cause |
R1090 |
T2035 |
T2036 |
amod |
red,cell |
R1091 |
T2036 |
T2038 |
compound |
cell,destruction |
R1092 |
T1976 |
T1977 |
det |
the,release |
R1093 |
T2037 |
T2036 |
compound |
blood,cell |
R1094 |
T2038 |
T2034 |
pobj |
destruction,with |
R1095 |
T2039 |
T2038 |
cc |
and,destruction |
R1096 |
T1977 |
T1973 |
attr |
release,is |
R1097 |
T2040 |
T2038 |
conj |
haemoglobinuria,destruction |
R1098 |
T2041 |
T2042 |
punct |
[,21 |
R1099 |
T1978 |
T1977 |
prep |
of,release |
R1100 |
T2042 |
T2025 |
parataxis |
21,cause |
R1101 |
T2043 |
T2042 |
punct |
],21 |
R1102 |
T1979 |
T1980 |
npadvmod |
hemoglobin,containing |
R1103 |
T2044 |
T2025 |
punct |
.,cause |
R1104 |
T2046 |
T2047 |
expl |
There,exist |
R1105 |
T1980 |
T1982 |
amod |
containing,exovesicles |
R1106 |
T2048 |
T2049 |
nummod |
two,types |
R1107 |
T2049 |
T2047 |
dobj |
types,exist |
R1108 |
T1981 |
T1980 |
punct |
-,containing |
R1109 |
T1982 |
T1978 |
pobj |
exovesicles,of |
R1110 |
T2050 |
T2049 |
prep |
of,types |
R1111 |
T2051 |
T2050 |
pobj |
vesicles,of |
R1112 |
T1983 |
T1977 |
acl |
occurring,release |
R1113 |
T2052 |
T2049 |
punct |
", ",types |
R1114 |
T2053 |
T2054 |
nmod |
micro,nanovesicles |
R1115 |
T1984 |
T1985 |
advmod |
in,vivo |
R1116 |
T2054 |
T2049 |
appos |
nanovesicles,types |
R1117 |
T2055 |
T2054 |
punct |
-,nanovesicles |
R1118 |
T2056 |
T2054 |
cc |
and,nanovesicles |
R1119 |
T1985 |
T1983 |
advmod |
vivo,occurring |
R1120 |
T2057 |
T2049 |
prep |
with,types |
R1121 |
T2058 |
T2059 |
det |
a,size |
R1122 |
T1986 |
T1987 |
advmod |
as,as |
R1123 |
T2059 |
T2057 |
pobj |
size,with |
R1124 |
T2060 |
T2059 |
prep |
of,size |
R1125 |
T1987 |
T1985 |
cc |
as,vivo |
R1126 |
T2061 |
T2062 |
nummod |
180,nm |
R1127 |
T2062 |
T2060 |
pobj |
nm,of |
R1128 |
T2063 |
T2062 |
cc |
and,nm |
R1129 |
T1988 |
T1987 |
advmod |
well,as |
R1130 |
T2064 |
T2065 |
nummod |
60,nm |
R1131 |
T2065 |
T2062 |
conj |
nm,nm |
R1132 |
T1989 |
T1990 |
advmod |
in,vitro |
R1133 |
T2066 |
T2047 |
punct |
", ",exist |
R1134 |
T2067 |
T2047 |
advmod |
respectively,exist |
R1135 |
T2068 |
T2047 |
punct |
.,exist |
R1136 |
T1990 |
T1985 |
conj |
vitro,vivo |
R1137 |
T2070 |
T2071 |
nsubjpass |
They,enriched |
R1138 |
T1991 |
T1992 |
punct |
[,20 |
R1139 |
T2072 |
T2071 |
auxpass |
are,enriched |
R1140 |
T2073 |
T2071 |
prep |
in,enriched |
R1141 |
T2074 |
T2075 |
npadvmod |
cholesterol,rich |
R1142 |
T2075 |
T2078 |
amod |
rich,domains |
R1143 |
T2076 |
T2074 |
cc |
and,cholesterol |
R1144 |
T1992 |
T1973 |
parataxis |
20,is |
R1145 |
T2077 |
T2074 |
conj |
sphingolipid,cholesterol |
R1146 |
T2078 |
T2073 |
pobj |
domains,in |
R1147 |
T2079 |
T2080 |
compound |
lipid,raft |
R1148 |
T1993 |
T1992 |
nummod |
7,20 |
R1149 |
T2080 |
T2078 |
compound |
raft,domains |
R1150 |
T2081 |
T2082 |
dep |
that,associated |
R1151 |
T2082 |
T2078 |
relcl |
associated,domains |
R1152 |
T1994 |
T1992 |
punct |
",",20 |
R1153 |
T2083 |
T2082 |
auxpass |
are,associated |
R1154 |
T2084 |
T2082 |
prep |
with,associated |
R1155 |
T1995 |
T1992 |
punct |
],20 |
R1156 |
T2085 |
T2084 |
pobj |
proteins,with |
R1157 |
T2086 |
T2085 |
prep |
like,proteins |
R1158 |
T1996 |
T1973 |
punct |
.,is |
R1159 |
T2087 |
T2086 |
pobj |
acetylcholinesterase,like |
R1160 |
T2088 |
T2087 |
punct |
", ",acetylcholinesterase |
R1161 |
T2089 |
T2090 |
compound |
cell,proteins |
R1162 |
T1998 |
T1999 |
det |
This,process |
R1163 |
T2090 |
T2087 |
conj |
proteins,acetylcholinesterase |
R1164 |
T2091 |
T2090 |
compound |
surface,proteins |
R1165 |
T2092 |
T2090 |
prep |
including,proteins |
R1166 |
T1999 |
T2000 |
nsubjpass |
process,thought |
R1167 |
T2093 |
T2094 |
det |
a,receptor |
R1168 |
T2094 |
T2092 |
pobj |
receptor,including |
R1169 |
T2095 |
T2094 |
compound |
complement,receptor |
R1170 |
T2001 |
T2000 |
auxpass |
is,thought |
R1171 |
T2096 |
T2090 |
punct |
", ",proteins |
R1172 |
T2097 |
T2090 |
cc |
and,proteins |
R1173 |
T2098 |
T2099 |
det |
the,proteins |
R1174 |
T2099 |
T2090 |
conj |
proteins,proteins |
R1175 |
T2002 |
T2003 |
aux |
to,represent |
R1176 |
T2100 |
T2101 |
compound |
lipid,raft |
R1177 |
T2101 |
T2099 |
compound |
raft,proteins |
R1178 |
T2102 |
T2099 |
appos |
stomatin,proteins |
R1179 |
T2003 |
T2000 |
xcomp |
represent,thought |
R1180 |
T2103 |
T2102 |
cc |
and,stomatin |
R1181 |
T2104 |
T2102 |
conj |
flotillin,stomatin |
R1182 |
T2105 |
T2071 |
punct |
", ",enriched |
R1183 |
T2004 |
T2005 |
det |
a,method |
R1184 |
T2106 |
T2071 |
cc |
but,enriched |
R1185 |
T2107 |
T2108 |
nsubj |
they,lack |
R1186 |
T2005 |
T2003 |
dobj |
method,represent |
R1187 |
T2108 |
T2071 |
conj |
lack,enriched |
R1188 |
T2109 |
T2110 |
det |
any,protein |
R1189 |
T2006 |
T2005 |
amod |
protective,method |
R1190 |
T2110 |
T2108 |
dobj |
protein,lack |
R1191 |
T2111 |
T2110 |
amod |
cytoskeletal,protein |
R1192 |
T2112 |
T2113 |
punct |
[,7 |
R1193 |
T2113 |
T2108 |
parataxis |
7,lack |
R1194 |
T2114 |
T2113 |
punct |
],7 |
R1195 |
T2115 |
T2108 |
punct |
.,lack |
R1196 |
T2007 |
T2005 |
prep |
of,method |
R1197 |
T2117 |
T2118 |
advmod |
Furthermore,contain |
R1198 |
T2008 |
T2009 |
det |
the,cell |
R1199 |
T2119 |
T2118 |
punct |
", ",contain |
R1200 |
T2120 |
T2118 |
nsubj |
they,contain |
R1201 |
T2009 |
T2007 |
pobj |
cell,of |
R1202 |
T2121 |
T2118 |
dobj |
sorcin,contain |
R1203 |
T2122 |
T2121 |
cc |
and,sorcin |
R1204 |
T2010 |
T2009 |
amod |
red,cell |
R1205 |
T2123 |
T2124 |
compound |
annexin,A7 |
R1206 |
T2124 |
T2121 |
conj |
A7,sorcin |
R1207 |
T2125 |
T2121 |
acl |
attached,sorcin |
R1208 |
T2011 |
T2009 |
compound |
blood,cell |
R1209 |
T2012 |
T2005 |
prep |
against,method |
R1210 |
T2126 |
T2125 |
prep |
to,attached |
R1211 |
T2013 |
T2014 |
det |
an,attack |
R1212 |
T2127 |
T2128 |
det |
the,rafts |
R1213 |
T2128 |
T2126 |
pobj |
rafts,to |
R1214 |
T2014 |
T2012 |
pobj |
attack,against |
R1216 |
T2130 |
T2118 |
punct |
.,contain |
R1217 |
T2015 |
T2014 |
prep |
by,attack |
R1218 |
T2016 |
T2017 |
prep |
for,components |
R1219 |
T2017 |
T2015 |
pobj |
components,by |
R1220 |
T2132 |
T2133 |
det |
Both,proteins |
R1221 |
T2133 |
T2134 |
nsubj |
proteins,are |
R1222 |
T2018 |
T2016 |
pobj |
example,for |
R1223 |
T2135 |
T2136 |
advmod |
more,abundant |
R1224 |
T2136 |
T2134 |
acomp |
abundant,are |
R1225 |
T2019 |
T2017 |
compound |
complement,components |
R1226 |
T2020 |
T2000 |
punct |
.,thought |
R1227 |
T2137 |
T2134 |
prep |
in,are |
R1228 |
T2138 |
T2137 |
pobj |
nanovesicles,in |
R1229 |
T2022 |
T2023 |
amod |
Red,cells |
R1230 |
T2139 |
T2134 |
punct |
.,are |
R1231 |
T2141 |
T2142 |
det |
The,formation |
R1232 |
T2142 |
T2144 |
nsubj |
formation,goes |
R1233 |
T2023 |
T2025 |
nsubj |
cells,cause |
R1234 |
T2143 |
T2142 |
compound |
vesicle,formation |
R1235 |
T2024 |
T2023 |
compound |
blood,cells |
R1236 |
T2145 |
T2144 |
prt |
along,goes |
R1237 |
T2146 |
T2144 |
prep |
with,goes |
R1238 |
T2147 |
T2148 |
amod |
several,changes |
R1239 |
T2148 |
T2146 |
pobj |
changes,with |
R1240 |
T2149 |
T2148 |
amod |
other,changes |
R1241 |
T2150 |
T2148 |
prep |
in,changes |
R1242 |
T2151 |
T2152 |
det |
the,cell |
R1243 |
T2152 |
T2150 |
pobj |
cell,in |
R1244 |
T2153 |
T2152 |
amod |
red,cell |
R1245 |
T2154 |
T2152 |
compound |
blood,cell |
R1246 |
T2155 |
T2148 |
prep |
like,changes |
R1247 |
T2156 |
T2157 |
amod |
cytoskeletal,rearrangements |
R1248 |
T2157 |
T2155 |
pobj |
rearrangements,like |
R1249 |
T2158 |
T2157 |
cc |
and,rearrangements |
R1250 |
T2159 |
T2157 |
conj |
changes,rearrangements |
R1251 |
T2160 |
T2159 |
prep |
in,changes |
R1252 |
T2161 |
T2162 |
det |
the,orientation |
R1253 |
T2162 |
T2160 |
pobj |
orientation,in |
R1254 |
T2163 |
T2162 |
compound |
phospholipid,orientation |
R1255 |
T2164 |
T2159 |
prep |
in,changes |
R1256 |
T2165 |
T2166 |
det |
the,membrane |
R1257 |
T2166 |
T2164 |
pobj |
membrane,in |
R1258 |
T2167 |
T2166 |
amod |
cellular,membrane |
R1259 |
T2168 |
T2144 |
punct |
.,goes |
R1264 |
T2443 |
T2444 |
compound |
Annexin,A7 |
R1265 |
T2444 |
T2445 |
compound |
A7,expression |
R1266 |
T2446 |
T2445 |
prep |
in,expression |
R1267 |
T2447 |
T2448 |
amod |
red,cells |
R1268 |
T2448 |
T2446 |
pobj |
cells,in |
R1269 |
T2449 |
T2448 |
compound |
blood,cells |
R1270 |
T2450 |
T2448 |
punct |
", ",cells |
R1271 |
T2451 |
T2452 |
amod |
red,cell |
R1272 |
T2452 |
T2454 |
npadvmod |
cell,derived |
R1273 |
T2453 |
T2452 |
compound |
blood,cell |
R1274 |
T2454 |
T2455 |
amod |
derived,exovesicles |
R1275 |
T2455 |
T2448 |
appos |
exovesicles,cells |
R1276 |
T2456 |
T2446 |
cc |
and,in |
R1277 |
T2457 |
T2446 |
conj |
in,in |
R1278 |
T2458 |
T2457 |
pobj |
platelets,in |
R1279 |
T2460 |
T2461 |
nsubj |
Salzer,reported |
R1280 |
T2462 |
T2463 |
advmod |
et,al. |
R1281 |
T2463 |
T2460 |
advmod |
al.,Salzer |
R1282 |
T2464 |
T2461 |
advmod |
recently,reported |
R1283 |
T2465 |
T2466 |
det |
the,presence |
R1284 |
T2466 |
T2461 |
dobj |
presence,reported |
R1285 |
T2467 |
T2466 |
prep |
of,presence |
R1286 |
T2468 |
T2469 |
det |
the,isoform |
R1287 |
T2469 |
T2467 |
pobj |
isoform,of |
R1288 |
T2470 |
T2471 |
nummod |
47,kDa |
R1289 |
T2471 |
T2469 |
compound |
kDa,isoform |
R1290 |
T2472 |
T2469 |
prep |
of,isoform |
R1291 |
T2473 |
T2474 |
compound |
annexin,A7 |
R1292 |
T2474 |
T2472 |
pobj |
A7,of |
R1293 |
T2475 |
T2474 |
cc |
and,A7 |
R1294 |
T2476 |
T2477 |
poss |
its,partner |
R1295 |
T2477 |
T2474 |
conj |
partner,A7 |
R1296 |
T2478 |
T2477 |
appos |
sorcin,partner |
R1297 |
T2479 |
T2469 |
prep |
in,isoform |
R1298 |
T2480 |
T2479 |
pobj |
micro,in |
R1299 |
T2481 |
T2480 |
punct |
-,micro |
R1300 |
T2482 |
T2480 |
cc |
and,micro |
R1301 |
T2483 |
T2480 |
conj |
nanovesicles,micro |
R1302 |
T2484 |
T2480 |
acl |
derived,micro |
R1303 |
T2485 |
T2484 |
prep |
from,derived |
R1304 |
T2486 |
T2487 |
amod |
red,cells |
R1305 |
T2487 |
T2485 |
pobj |
cells,from |
R1306 |
T2488 |
T2487 |
compound |
blood,cells |
R1307 |
T2489 |
T2490 |
advmod |
where,located |
R1308 |
T2490 |
T2487 |
relcl |
located,cells |
R1309 |
T2491 |
T2490 |
nsubjpass |
they,located |
R1310 |
T2492 |
T2490 |
auxpass |
are,located |
R1311 |
T2493 |
T2490 |
prep |
in,located |
R1312 |
T2494 |
T2495 |
det |
the,lumen |
R1313 |
T2495 |
T2493 |
pobj |
lumen,in |
R1314 |
T2496 |
T2490 |
cc |
and,located |
R1315 |
T2497 |
T2498 |
auxpass |
are,enriched |
R1316 |
T2498 |
T2490 |
conj |
enriched,located |
R1317 |
T2499 |
T2498 |
advmod |
also,enriched |
R1318 |
T2500 |
T2498 |
prep |
in,enriched |
R1319 |
T2501 |
T2502 |
compound |
membrane,rafts |
R1320 |
T2502 |
T2500 |
pobj |
rafts,in |
R1321 |
T2503 |
T2504 |
punct |
[,7 |
R1322 |
T2504 |
T2461 |
parataxis |
7,reported |
R1323 |
T2505 |
T2504 |
punct |
],7 |
R1324 |
T2506 |
T2461 |
punct |
.,reported |
R1325 |
T2508 |
T2509 |
nsubj |
We,extended |
R1326 |
T2510 |
T2509 |
aux |
have,extended |
R1327 |
T2511 |
T2509 |
advmod |
now,extended |
R1335 |
T2519 |
T2520 |
det |
the,isoform |
R1336 |
T2520 |
T2518 |
nsubj |
isoform,is |
R1337 |
T2521 |
T2522 |
nummod |
51,kDa |
R1338 |
T2522 |
T2520 |
compound |
kDa,isoform |
R1339 |
T2523 |
T2518 |
acomp |
present,is |
R1340 |
T2524 |
T2525 |
advmod |
as,well |
R1341 |
T2525 |
T2518 |
advmod |
well,is |
R1342 |
T2526 |
T2509 |
punct |
.,extended |
R1343 |
T2528 |
T2529 |
prep |
In,detected |
R1344 |
T2530 |
T2531 |
amod |
western,blots |
R1345 |
T2531 |
T2528 |
pobj |
blots,In |
R1346 |
T2532 |
T2531 |
prep |
of,blots |
R1347 |
T2533 |
T2534 |
amod |
human,cells |
R1348 |
T2534 |
T2532 |
pobj |
cells,of |
R1349 |
T2535 |
T2534 |
amod |
red,cells |
R1350 |
T2536 |
T2534 |
compound |
blood,cells |
R1351 |
T2537 |
T2538 |
det |
the,isoforms |
R1352 |
T2538 |
T2529 |
nsubjpass |
isoforms,detected |
R1353 |
T2539 |
T2540 |
nummod |
47,kDa |
R1354 |
T2540 |
T2538 |
compound |
kDa,isoforms |
R1355 |
T2541 |
T2539 |
cc |
and,47 |
R1356 |
T2542 |
T2539 |
conj |
51,47 |
R1357 |
T2543 |
T2529 |
auxpass |
were,detected |
R1358 |
T2544 |
T2529 |
advcl |
using,detected |
R1359 |
T2545 |
T2544 |
dobj |
mAb,using |
R1360 |
T2546 |
T2545 |
nummod |
203–217,mAb |
R1361 |
T2547 |
T2548 |
punct |
(,Fig. |
R1362 |
T2548 |
T2529 |
parataxis |
Fig.,detected |
R1363 |
T2549 |
T2548 |
nummod |
1,Fig. |
R1364 |
T2550 |
T2548 |
punct |
),Fig. |
R1365 |
T2551 |
T2529 |
punct |
.,detected |
R1366 |
T2553 |
T2554 |
det |
The,isoform |
R1367 |
T2554 |
T2557 |
nsubjpass |
isoform,detected |
R1368 |
T2555 |
T2556 |
nummod |
47,kDa |
R1369 |
T2556 |
T2554 |
compound |
kDa,isoform |
R1370 |
T2558 |
T2557 |
auxpass |
was,detected |
R1371 |
T2559 |
T2557 |
prep |
in,detected |
R1372 |
T2560 |
T2561 |
npadvmod |
silver,stained |
R1373 |
T2561 |
T2562 |
amod |
stained,gels |
R1374 |
T2562 |
T2559 |
pobj |
gels,in |
R1375 |
T2563 |
T2557 |
cc |
and,detected |
R1376 |
T2564 |
T2565 |
poss |
its,identity |
R1377 |
T2565 |
T2566 |
nsubj |
identity,confirmed |
R1378 |
T2566 |
T2557 |
conj |
confirmed,detected |
R1379 |
T2567 |
T2565 |
prep |
with,identity |
R1380 |
T2568 |
T2569 |
compound |
annexin,A7 |
R1381 |
T2569 |
T2567 |
pobj |
A7,with |
R1382 |
T2570 |
T2566 |
prep |
by,confirmed |
R1383 |
T2571 |
T2572 |
compound |
peptide,fingerprinting |
R1384 |
T2572 |
T2570 |
pobj |
fingerprinting,by |
R1385 |
T2573 |
T2572 |
compound |
mass,fingerprinting |
R1386 |
T2574 |
T2575 |
punct |
(,shown |
R1387 |
T2575 |
T2566 |
parataxis |
shown,confirmed |
R1388 |
T2576 |
T2575 |
nsubj |
data,shown |
R1389 |
T2577 |
T2575 |
neg |
not,shown |
R1390 |
T2578 |
T2575 |
punct |
),shown |
R1391 |
T2579 |
T2566 |
punct |
.,confirmed |
R1392 |
T2581 |
T2582 |
det |
The,isoform |
R1393 |
T2582 |
T2585 |
nsubjpass |
isoform,detected |
R1394 |
T2583 |
T2584 |
nummod |
51,kDa |
R1395 |
T2584 |
T2582 |
compound |
kDa,isoform |
R1396 |
T2586 |
T2585 |
auxpass |
was,detected |
R1397 |
T2587 |
T2585 |
advmod |
only,detected |
R1398 |
T2588 |
T2585 |
prep |
in,detected |
R1399 |
T2589 |
T2590 |
amod |
western,blots |
R1400 |
T2590 |
T2588 |
pobj |
blots,in |
R1401 |
T2591 |
T2585 |
punct |
.,detected |
R1420 |
T2613 |
T2614 |
det |
a,role |
R1421 |
T2614 |
T2604 |
dobj |
role,plays |
R1422 |
T2615 |
T2604 |
prep |
in,plays |
R1423 |
T2616 |
T2617 |
compound |
membrane,organization |
R1424 |
T2617 |
T2615 |
pobj |
organization,in |
R1425 |
T2618 |
T2617 |
cc |
and,organization |
R1426 |
T2619 |
T2620 |
det |
the,process |
R1427 |
T2620 |
T2617 |
conj |
process,organization |
R1428 |
T2621 |
T2620 |
compound |
vesiculation,process |
R1429 |
T2622 |
T2596 |
punct |
.,led |
R1430 |
T2624 |
T2625 |
aux |
To,examine |
R1431 |
T2625 |
T2626 |
advcl |
examine,analysed |
R1432 |
T2627 |
T2625 |
dobj |
this,examine |
R1433 |
T2628 |
T2626 |
punct |
", ",analysed |
R1434 |
T2629 |
T2626 |
nsubj |
we,analysed |
R1435 |
T2630 |
T2631 |
det |
the,ability |
R1436 |
T2631 |
T2626 |
dobj |
ability,analysed |
R1437 |
T2632 |
T2631 |
prep |
of,ability |
R1438 |
T2633 |
T2634 |
amod |
red,cells |
R1439 |
T2634 |
T2632 |
pobj |
cells,of |
R1440 |
T2635 |
T2634 |
compound |
blood,cells |
R1441 |
T2636 |
T2634 |
acl |
derived,cells |
R1442 |
T2637 |
T2636 |
prep |
from,derived |
R1443 |
T2638 |
T2639 |
det |
the,mice |
R1444 |
T2639 |
T2637 |
pobj |
mice,from |
R1445 |
T2640 |
T2641 |
compound |
annexin,A7 |
R1446 |
T2641 |
T2639 |
compound |
A7,mice |
R1447 |
T2642 |
T2643 |
compound |
knock,out |
R1448 |
T2643 |
T2639 |
compound |
out,mice |
R1449 |
T2644 |
T2645 |
punct |
(,anxA7 |
R1450 |
T2645 |
T2639 |
parataxis |
anxA7,mice |
R1451 |
T2646 |
T2645 |
punct |
-,anxA7 |
R1452 |
T2647 |
T2645 |
punct |
/,anxA7 |
R1453 |
T2648 |
T2645 |
punct |
-,anxA7 |
R1454 |
T2649 |
T2645 |
punct |
),anxA7 |
R1455 |
T2650 |
T2651 |
aux |
to,form |
R1456 |
T2651 |
T2631 |
acl |
form,ability |
R1457 |
T2652 |
T2651 |
dobj |
exovesicles,form |
R1458 |
T2653 |
T2626 |
punct |
.,analysed |
R1459 |
T2655 |
T2656 |
prep |
Because,included |
R1460 |
T2657 |
T2655 |
pcomp |
of,Because |
R1461 |
T2658 |
T2659 |
amod |
better,accessibility |
R1462 |
T2659 |
T2655 |
pobj |
accessibility,Because |
R1463 |
T2660 |
T2659 |
cc |
and,accessibility |
R1464 |
T2661 |
T2662 |
amod |
larger,amounts |
R1465 |
T2662 |
T2659 |
conj |
amounts,accessibility |
R1466 |
T2663 |
T2662 |
amod |
available,amounts |
R1467 |
T2664 |
T2665 |
dep |
that,led |
R1468 |
T2665 |
T2659 |
relcl |
led,accessibility |
R1469 |
T2666 |
T2665 |
prep |
to,led |
R1470 |
T2667 |
T2668 |
amod |
clear,results |
R1471 |
T2668 |
T2666 |
pobj |
results,to |
R1472 |
T2669 |
T2656 |
punct |
", ",included |
R1473 |
T2670 |
T2656 |
nsubj |
we,included |
R1474 |
T2671 |
T2672 |
poss |
our,data |
R1475 |
T2672 |
T2656 |
dobj |
data,included |
R1476 |
T2673 |
T2672 |
acl |
obtained,data |
R1477 |
T2674 |
T2673 |
prep |
with,obtained |
R1478 |
T2675 |
T2676 |
amod |
human,blood |
R1479 |
T2676 |
T2674 |
pobj |
blood,with |
R1480 |
T2677 |
T2656 |
punct |
.,included |
R1481 |
T2679 |
T2680 |
advcl |
Independent,released |
R1482 |
T2681 |
T2679 |
prep |
of,Independent |
R1483 |
T2682 |
T2683 |
det |
the,presence |
R1484 |
T2683 |
T2681 |
pobj |
presence,of |
R1485 |
T2684 |
T2683 |
prep |
of,presence |
R1486 |
T2685 |
T2686 |
compound |
annexin,A7 |
R1487 |
T2686 |
T2684 |
pobj |
A7,of |
R1488 |
T2687 |
T2688 |
det |
both,types |
R1489 |
T2688 |
T2680 |
nsubjpass |
types,released |
R1490 |
T2689 |
T2688 |
prep |
of,types |
R1491 |
T2690 |
T2689 |
pobj |
exovesicles,of |
R1492 |
T2691 |
T2688 |
punct |
", ",types |
R1493 |
T2692 |
T2693 |
nmod |
micro,nanovesicles |
R1494 |
T2693 |
T2688 |
appos |
nanovesicles,types |
R1495 |
T2694 |
T2693 |
punct |
-,nanovesicles |
R1496 |
T2695 |
T2693 |
cc |
and,nanovesicles |
R1497 |
T2696 |
T2680 |
punct |
", ",released |
R1498 |
T2697 |
T2680 |
auxpass |
were,released |
R1499 |
T2698 |
T2680 |
prep |
after,released |
R1500 |
T2699 |
T2700 |
compound |
Ca2+,ionophore |
R1501 |
T2700 |
T2702 |
compound |
ionophore,treatment |
R1502 |
T2701 |
T2700 |
punct |
/,ionophore |
R1503 |
T2702 |
T2698 |
pobj |
treatment,after |
R1504 |
T2703 |
T2704 |
mark |
as,determined |
R1505 |
T2704 |
T2680 |
advcl |
determined,released |
R1506 |
T2705 |
T2704 |
agent |
by,determined |
R1507 |
T2706 |
T2707 |
compound |
acetylcholine,esterase |
R1508 |
T2707 |
T2708 |
compound |
esterase,activity |
R1509 |
T2708 |
T2705 |
pobj |
activity,by |
R1510 |
T2709 |
T2708 |
cc |
and,activity |
R1511 |
T2710 |
T2711 |
amod |
microscopic,examination |
R1512 |
T2711 |
T2708 |
conj |
examination,activity |
R1513 |
T2712 |
T2680 |
punct |
.,released |
R1514 |
T2714 |
T2715 |
compound |
Annexin,A7 |
R1515 |
T2715 |
T2716 |
nsubj |
A7,is |
R1516 |
T2717 |
T2716 |
acomp |
present,is |
R1517 |
T2718 |
T2716 |
prep |
in,is |
R1518 |
T2719 |
T2720 |
preconj |
both,types |
R1519 |
T2720 |
T2718 |
pobj |
types,in |
R1520 |
T2721 |
T2720 |
compound |
vesicle,types |
R1521 |
T2722 |
T2723 |
advmod |
where,is |
R1522 |
T2723 |
T2720 |
advcl |
is,types |
R1523 |
T2724 |
T2723 |
nsubj |
it,is |
R1524 |
T2725 |
T2726 |
advmod |
more,enriched |
R1525 |
T2726 |
T2723 |
acomp |
enriched,is |
R1526 |
T2727 |
T2723 |
prep |
in,is |
R1527 |
T2728 |
T2727 |
pobj |
nanovesicles,in |
R1528 |
T2729 |
T2730 |
punct |
(,Fig. |
R1529 |
T2730 |
T2716 |
parataxis |
Fig.,is |
R1530 |
T2731 |
T2730 |
nummod |
2,Fig. |
R1531 |
T2732 |
T2730 |
punct |
),Fig. |
R1532 |
T2733 |
T2716 |
punct |
.,is |
R1533 |
T2735 |
T2736 |
det |
The,isoform |
R1534 |
T2736 |
T2739 |
nsubj |
isoform,is |
R1535 |
T2737 |
T2738 |
nummod |
51,kDa |
R1536 |
T2738 |
T2736 |
compound |
kDa,isoform |
R1537 |
T2740 |
T2739 |
advmod |
only,is |
R1538 |
T2741 |
T2739 |
acomp |
detectable,is |
R1539 |
T2742 |
T2739 |
prep |
in,is |
R1540 |
T2743 |
T2742 |
pobj |
nanovesicles,in |
R1541 |
T2744 |
T2739 |
punct |
.,is |
R1542 |
T2746 |
T2747 |
det |
The,quantity |
R1543 |
T2747 |
T2748 |
nsubj |
quantity,differ |
R1544 |
T2749 |
T2747 |
prep |
of,quantity |
R1545 |
T2750 |
T2751 |
det |
both,types |
R1546 |
T2751 |
T2749 |
pobj |
types,of |
R1547 |
T2752 |
T2751 |
compound |
vesicle,types |
R1548 |
T2753 |
T2748 |
aux |
did,differ |
R1549 |
T2754 |
T2748 |
neg |
not,differ |
R1550 |
T2755 |
T2748 |
prep |
between,differ |
R1551 |
T2756 |
T2757 |
amod |
wild,type |
R1552 |
T2757 |
T2758 |
nmod |
type,vesicles |
R1553 |
T2758 |
T2755 |
pobj |
vesicles,between |
R1554 |
T2759 |
T2757 |
cc |
and,type |
R1555 |
T2760 |
T2761 |
compound |
knock,out |
R1556 |
T2761 |
T2757 |
conj |
out,type |
R1557 |
T2762 |
T2763 |
amod |
red,cell |
R1558 |
T2763 |
T2758 |
compound |
cell,vesicles |
R1559 |
T2764 |
T2763 |
compound |
blood,cell |
R1560 |
T2765 |
T2766 |
mark |
as,determined |
R1561 |
T2766 |
T2748 |
advcl |
determined,differ |
R1562 |
T2767 |
T2766 |
agent |
by,determined |
R1563 |
T2768 |
T2769 |
det |
the,values |
R1564 |
T2769 |
T2767 |
pobj |
values,by |
R1565 |
T2770 |
T2769 |
compound |
AChE,values |
R1566 |
T2771 |
T2769 |
punct |
-,values |
R1567 |
T2772 |
T2773 |
punct |
(,both |
R1568 |
T2773 |
T2769 |
parataxis |
both,values |
R1569 |
T2774 |
T2775 |
compound |
A405,nanovesicles |
R1570 |
T2775 |
T2776 |
dep |
nanovesicles,0.18 |
R1571 |
T2776 |
T2773 |
dep |
0.18,both |
R1572 |
T2777 |
T2776 |
punct |
: ,0.18 |
R1573 |
T2778 |
T2776 |
punct |
~,0.18 |
R1574 |
T2779 |
T2773 |
punct |
;,both |
R1575 |
T2780 |
T2781 |
compound |
A405,microvesicles |
R1576 |
T2781 |
T2782 |
dep |
microvesicles,0.9 |
R1577 |
T2782 |
T2773 |
dep |
0.9,both |
R1578 |
T2783 |
T2782 |
punct |
: ,0.9 |
R1579 |
T2784 |
T2782 |
punct |
~,0.9 |
R1580 |
T2785 |
T2773 |
punct |
;,both |
R1581 |
T2786 |
T2773 |
punct |
", ",both |
R1596 |
T2803 |
T2802 |
nsubjpass |
it,described |
R1597 |
T2804 |
T2802 |
aux |
has,described |
R1598 |
T2805 |
T2802 |
auxpass |
been,described |
R1599 |
T2806 |
T2807 |
aux |
to,fuse |
R1600 |
T2807 |
T2802 |
xcomp |
fuse,described |
R1601 |
T2808 |
T2807 |
dobj |
membranes,fuse |
R1602 |
T2809 |
T2797 |
punct |
", ",is |
R1603 |
T2810 |
T2797 |
neg |
not,is |
R1604 |
T2811 |
T2812 |
det |
a,component |
R1605 |
T2812 |
T2797 |
attr |
component,is |
R1606 |
T2813 |
T2812 |
amod |
key,component |
R1607 |
T2814 |
T2812 |
prep |
in,component |
R1608 |
T2815 |
T2816 |
det |
the,process |
R1609 |
T2816 |
T2814 |
pobj |
process,in |
R1610 |
T2817 |
T2816 |
prep |
of,process |
R1611 |
T2818 |
T2819 |
det |
the,formation |
R1612 |
T2819 |
T2817 |
pobj |
formation,of |
R1613 |
T2820 |
T2819 |
prep |
of,formation |
R1614 |
T2821 |
T2822 |
amod |
red,cell |
R1615 |
T2822 |
T2824 |
compound |
cell,exovesicles |
R1616 |
T2823 |
T2822 |
compound |
blood,cell |
R1617 |
T2824 |
T2820 |
pobj |
exovesicles,of |
R1618 |
T2825 |
T2795 |
punct |
.,appears |
R1619 |
T2827 |
T2828 |
advmod |
When,probed |
R1620 |
T2828 |
T2830 |
advcl |
probed,found |
R1621 |
T2829 |
T2828 |
nsubj |
we,probed |
R1622 |
T2831 |
T2828 |
prep |
for,probed |
R1623 |
T2832 |
T2833 |
det |
the,presence |
R1624 |
T2833 |
T2831 |
pobj |
presence,for |
R1625 |
T2834 |
T2833 |
prep |
of,presence |
R1626 |
T2835 |
T2834 |
pobj |
sorcin,of |
R1627 |
T2836 |
T2828 |
prep |
in,probed |
R1628 |
T2837 |
T2838 |
amod |
wild,type |
R1629 |
T2838 |
T2839 |
nmod |
type,vesicles |
R1630 |
T2839 |
T2836 |
pobj |
vesicles,in |
R1631 |
T2840 |
T2838 |
cc |
and,type |
R1632 |
T2841 |
T2838 |
conj |
mutant,type |
R1633 |
T2842 |
T2830 |
nsubj |
we,found |
R1634 |
T2843 |
T2844 |
mark |
that,reduced |
R1635 |
T2844 |
T2830 |
ccomp |
reduced,found |
R1636 |
T2845 |
T2846 |
det |
the,levels |
R1637 |
T2846 |
T2844 |
nsubjpass |
levels,reduced |
R1638 |
T2847 |
T2846 |
compound |
sorcin,levels |
R1639 |
T2848 |
T2844 |
auxpass |
were,reduced |
R1640 |
T2849 |
T2844 |
prep |
in,reduced |
R1641 |
T2850 |
T2851 |
det |
the,nanovesicles |
R1642 |
T2851 |
T2849 |
pobj |
nanovesicles,in |
R1643 |
T2852 |
T2851 |
amod |
mutant,nanovesicles |
R1644 |
T2853 |
T2854 |
punct |
(,Fig. |
R1645 |
T2854 |
T2830 |
parataxis |
Fig.,found |
R1646 |
T2855 |
T2854 |
nummod |
2,Fig. |
R1647 |
T2856 |
T2854 |
punct |
),Fig. |
R1648 |
T2857 |
T2830 |
punct |
.,found |
R1664 |
T2875 |
T2874 |
compound |
iodixanol,gradients |
R1665 |
T2876 |
T2874 |
compound |
density,gradients |
R1666 |
T2877 |
T2861 |
punct |
.,used |
R1667 |
T2879 |
T2880 |
det |
Both,isoforms |
R1668 |
T2880 |
T2881 |
nsubj |
isoforms,were |
R1669 |
T2882 |
T2881 |
acomp |
present,were |
R1670 |
T2883 |
T2881 |
prep |
in,were |
R1671 |
T2884 |
T2885 |
det |
the,fraction |
R1672 |
T2885 |
T2883 |
pobj |
fraction,in |
R1673 |
T2886 |
T2885 |
amod |
soluble,fraction |
R1674 |
T2887 |
T2888 |
advmod |
as,as |
R1675 |
T2888 |
T2883 |
cc |
as,in |
R1676 |
T2889 |
T2888 |
advmod |
well,as |
R1677 |
T2890 |
T2883 |
conj |
in,in |
R1678 |
T2891 |
T2892 |
det |
the,fractions |
R1679 |
T2892 |
T2890 |
pobj |
fractions,in |
R1680 |
T2893 |
T2892 |
compound |
gradient,fractions |
R1681 |
T2894 |
T2895 |
advmod |
where,assumed |
R1682 |
T2895 |
T2892 |
relcl |
assumed,fractions |
R1683 |
T2896 |
T2895 |
nsubjpass |
they,assumed |
R1684 |
T2897 |
T2895 |
auxpass |
are,assumed |
R1685 |
T2898 |
T2899 |
aux |
to,associated |
R1686 |
T2899 |
T2895 |
xcomp |
associated,assumed |
R1687 |
T2900 |
T2899 |
auxpass |
be,associated |
R1688 |
T2901 |
T2899 |
prep |
with,associated |
R1689 |
T2902 |
T2901 |
pobj |
membranes,with |
R1690 |
T2903 |
T2881 |
punct |
.,were |
R1691 |
T2905 |
T2906 |
det |
These,membranes |
R1692 |
T2906 |
T2907 |
nsubj |
membranes,are |
R1693 |
T2908 |
T2907 |
advmod |
exclusively,are |
R1694 |
T2909 |
T2910 |
compound |
plasma,membranes |
R1695 |
T2910 |
T2907 |
attr |
membranes,are |
R1696 |
T2911 |
T2912 |
mark |
as,are |
R1697 |
T2912 |
T2907 |
advcl |
are,are |
R1698 |
T2913 |
T2914 |
amod |
red,cells |
R1699 |
T2914 |
T2912 |
nsubj |
cells,are |
R1700 |
T2915 |
T2914 |
compound |
blood,cells |
R1701 |
T2916 |
T2912 |
acomp |
free,are |
R1702 |
T2917 |
T2916 |
prep |
of,free |
R1703 |
T2918 |
T2919 |
det |
any,organelles |
R1704 |
T2919 |
T2917 |
pobj |
organelles,of |
R1705 |
T2920 |
T2907 |
punct |
.,are |
R1706 |
T2922 |
T2923 |
det |
The,distribution |
R1707 |
T2923 |
T2924 |
nsubj |
distribution,was |
R1708 |
T2925 |
T2924 |
advmod |
however,was |
R1709 |
T2926 |
T2924 |
neg |
not,was |
R1710 |
T2927 |
T2924 |
acomp |
homogeneous,was |
R1711 |
T2928 |
T2924 |
prep |
throughout,was |
R1712 |
T2929 |
T2930 |
det |
the,gradient |
R1713 |
T2930 |
T2928 |
pobj |
gradient,throughout |
R1714 |
T2931 |
T2924 |
punct |
.,was |
R1715 |
T2933 |
T2934 |
nsubj |
This,reflect |
R1716 |
T2935 |
T2934 |
aux |
could,reflect |
R1717 |
T2936 |
T2937 |
det |
the,binding |
R1718 |
T2937 |
T2934 |
dobj |
binding,reflect |
R1719 |
T2938 |
T2937 |
prep |
of,binding |
R1720 |
T2939 |
T2940 |
compound |
annexin,A7 |
R1721 |
T2940 |
T2938 |
pobj |
A7,of |
R1722 |
T2941 |
T2937 |
prep |
to,binding |
R1723 |
T2942 |
T2943 |
compound |
membrane,subdomains |
R1724 |
T2943 |
T2941 |
pobj |
subdomains,to |
R1725 |
T2944 |
T2945 |
dep |
that,have |
R1726 |
T2945 |
T2943 |
relcl |
have,subdomains |
R1727 |
T2946 |
T2947 |
amod |
different,composition |
R1728 |
T2947 |
T2945 |
dobj |
composition,have |
R1729 |
T2948 |
T2947 |
nmod |
lipid,composition |
R1730 |
T2949 |
T2948 |
cc |
or,lipid |
R1731 |
T2950 |
T2951 |
compound |
lipid,protein |
R1732 |
T2951 |
T2948 |
conj |
protein,lipid |
R1733 |
T2952 |
T2951 |
punct |
/,protein |
R1734 |
T2953 |
T2954 |
mark |
as,discussed |
R1735 |
T2954 |
T2934 |
advcl |
discussed,reflect |
R1736 |
T2955 |
T2954 |
agent |
by,discussed |
R1737 |
T2956 |
T2955 |
pobj |
Salzer,by |
R1738 |
T2957 |
T2958 |
advmod |
et,al. |
R1739 |
T2958 |
T2956 |
advmod |
al.,Salzer |
R1740 |
T2959 |
T2960 |
punct |
[,7 |
R1741 |
T2960 |
T2934 |
parataxis |
7,reflect |
R1742 |
T2961 |
T2960 |
punct |
],7 |
R1743 |
T2962 |
T2934 |
punct |
.,reflect |
R1744 |
T2964 |
T2965 |
advmod |
Likewise,exhibit |
R1745 |
T2966 |
T2965 |
punct |
", ",exhibit |
R1746 |
T2967 |
T2965 |
nsubj |
sorcin,exhibit |
R1747 |
T2968 |
T2965 |
aux |
does,exhibit |
R1748 |
T2969 |
T2965 |
neg |
not,exhibit |
R1749 |
T2970 |
T2971 |
det |
a,distribution |
R1750 |
T2971 |
T2965 |
dobj |
distribution,exhibit |
R1751 |
T2972 |
T2971 |
amod |
homogeneous,distribution |
R1752 |
T2973 |
T2965 |
prep |
in,exhibit |
R1753 |
T2974 |
T2975 |
det |
the,gradient |
R1754 |
T2975 |
T2973 |
pobj |
gradient,in |
R1755 |
T2976 |
T2965 |
punct |
.,exhibit |
R1756 |
T2978 |
T2979 |
advmod |
Moreover,segregates |
R1757 |
T2980 |
T2979 |
punct |
", ",segregates |
R1758 |
T2981 |
T2979 |
nsubj |
it,segregates |
R1759 |
T2982 |
T2979 |
prep |
into,segregates |
R1760 |
T2983 |
T2982 |
pobj |
vesicles,into |
R1761 |
T2984 |
T2985 |
dep |
which,associated |
R1762 |
T2985 |
T2983 |
relcl |
associated,vesicles |
R1763 |
T2986 |
T2985 |
auxpass |
are,associated |
R1764 |
T2987 |
T2985 |
neg |
not,associated |
R1765 |
T2988 |
T2985 |
prep |
with,associated |
R1766 |
T2989 |
T2990 |
compound |
annexin,A7 |
R1767 |
T2990 |
T2988 |
pobj |
A7,with |
R1768 |
T2991 |
T2992 |
punct |
(,Fig. |
R1769 |
T2992 |
T2979 |
parataxis |
Fig.,segregates |
R1770 |
T2993 |
T2992 |
nummod |
3,Fig. |
R1771 |
T2994 |
T2992 |
punct |
),Fig. |
R1772 |
T2995 |
T2979 |
punct |
.,segregates |
R1773 |
T2997 |
T2998 |
nsubj |
We,tested |
R1774 |
T2999 |
T2998 |
advmod |
also,tested |
R1775 |
T3000 |
T2998 |
dobj |
platelets,tested |
R1776 |
T3001 |
T2998 |
prep |
for,tested |
R1777 |
T3002 |
T3003 |
compound |
annexin,A7 |
R1778 |
T3003 |
T3004 |
compound |
A7,expression |
R1779 |
T3004 |
T3001 |
pobj |
expression,for |
R1780 |
T3005 |
T2998 |
cc |
and,tested |
R1781 |
T3006 |
T2998 |
conj |
detected,tested |
R1782 |
T3007 |
T3008 |
det |
the,isoform |
R1783 |
T3008 |
T3006 |
dobj |
isoform,detected |
R1784 |
T3009 |
T3010 |
nummod |
47,kDa |
R1785 |
T3010 |
T3008 |
compound |
kDa,isoform |
R1786 |
T3011 |
T3012 |
punct |
(,Fig. |
R1787 |
T3012 |
T3006 |
parataxis |
Fig.,detected |
R1788 |
T3013 |
T3012 |
nummod |
1,Fig. |
R1789 |
T3014 |
T3012 |
punct |
),Fig. |
R1790 |
T3015 |
T2998 |
punct |
.,tested |
R1795 |
T3280 |
T3281 |
det |
The,lack |
R1796 |
T3281 |
T3282 |
nsubj |
lack,changes |
R1797 |
T3283 |
T3281 |
prep |
of,lack |
R1798 |
T3284 |
T3285 |
compound |
annexin,A7 |
R1799 |
T3285 |
T3283 |
pobj |
A7,of |
R1800 |
T3286 |
T3287 |
amod |
red,cell |
R1801 |
T3287 |
T3282 |
dobj |
cell,changes |
R1802 |
T3288 |
T3287 |
compound |
blood,cell |
R1803 |
T3289 |
T3287 |
appos |
morphology,cell |
R1804 |
T3290 |
T3289 |
cc |
and,morphology |
R1805 |
T3291 |
T3292 |
amod |
osmotic,resistance |
R1806 |
T3292 |
T3289 |
conj |
resistance,morphology |
R1807 |
T3294 |
T3295 |
mark |
As,is |
R1808 |
T3295 |
T3298 |
advcl |
is,studied |
R1809 |
T3296 |
T3297 |
compound |
annexin,A7 |
R1810 |
T3297 |
T3295 |
nsubj |
A7,is |
R1811 |
T3299 |
T3300 |
det |
a,component |
R1812 |
T3300 |
T3295 |
attr |
component,is |
R1813 |
T3301 |
T3300 |
prep |
of,component |
R1814 |
T3302 |
T3303 |
amod |
red,cell |
R1815 |
T3303 |
T3305 |
compound |
cell,vesicles |
R1816 |
T3304 |
T3303 |
compound |
blood,cell |
R1817 |
T3305 |
T3301 |
pobj |
vesicles,of |
R1818 |
T3306 |
T3298 |
nsubj |
we,studied |
R1819 |
T3307 |
T3308 |
det |
the,consequences |
R1820 |
T3308 |
T3298 |
dobj |
consequences,studied |
R1821 |
T3309 |
T3308 |
prep |
of,consequences |
R1822 |
T3310 |
T3311 |
det |
the,loss |
R1823 |
T3311 |
T3309 |
pobj |
loss,of |
R1824 |
T3312 |
T3311 |
prep |
of,loss |
R1825 |
T3313 |
T3314 |
det |
the,protein |
R1826 |
T3314 |
T3312 |
pobj |
protein,of |
R1827 |
T3315 |
T3298 |
prep |
in,studied |
R1828 |
T3316 |
T3317 |
det |
the,mouse |
R1829 |
T3317 |
T3315 |
pobj |
mouse,in |
R1830 |
T3318 |
T3317 |
nmod |
anxA7,mouse |
R1831 |
T3319 |
T3318 |
punct |
-,anxA7 |
R1832 |
T3320 |
T3318 |
punct |
/,anxA7 |
R1833 |
T3321 |
T3318 |
punct |
-,anxA7 |
R1834 |
T3322 |
T3298 |
prep |
for,studied |
R1835 |
T3323 |
T3324 |
amod |
red,cell |
R1836 |
T3324 |
T3322 |
pobj |
cell,for |
R1837 |
T3325 |
T3324 |
compound |
blood,cell |
R1838 |
T3326 |
T3324 |
appos |
morphology,cell |
R1839 |
T3327 |
T3326 |
cc |
and,morphology |
R1840 |
T3328 |
T3329 |
amod |
osmotic,resistance |
R1841 |
T3329 |
T3326 |
conj |
resistance,morphology |
R1842 |
T3330 |
T3298 |
punct |
.,studied |
R1843 |
T3332 |
T3333 |
prep |
In,revealed |
R1844 |
T3334 |
T3335 |
det |
a,smear |
R1845 |
T3335 |
T3332 |
pobj |
smear,In |
R1846 |
T3336 |
T3335 |
amod |
standard,smear |
R1847 |
T3337 |
T3335 |
punct |
',smear |
R1848 |
T3338 |
T3335 |
compound |
blood,smear |
R1849 |
T3339 |
T3333 |
punct |
',revealed |
R1850 |
T3340 |
T3341 |
nsubj |
we,note |
R1851 |
T3341 |
T3333 |
ccomp |
note,revealed |
R1852 |
T3342 |
T3341 |
aux |
did,note |
R1853 |
T3343 |
T3341 |
neg |
not,note |
R1854 |
T3344 |
T3345 |
det |
a,deformation |
R1855 |
T3345 |
T3341 |
dobj |
deformation,note |
R1856 |
T3346 |
T3345 |
prep |
of,deformation |
R1857 |
T3347 |
T3348 |
det |
the,cells |
R1858 |
T3348 |
T3346 |
pobj |
cells,of |
R1859 |
T3349 |
T3333 |
punct |
", ",revealed |
R1860 |
T3350 |
T3333 |
advmod |
however,revealed |
R1861 |
T3351 |
T3352 |
amod |
dark,field |
R1862 |
T3352 |
T3353 |
compound |
field,microscopy |
R1863 |
T3353 |
T3333 |
nsubj |
microscopy,revealed |
R1864 |
T3354 |
T3333 |
dobj |
changes,revealed |
R1865 |
T3355 |
T3354 |
prep |
in,changes |
R1866 |
T3356 |
T3355 |
pobj |
shape,in |
R1867 |
T3357 |
T3356 |
cc |
and,shape |
R1868 |
T3358 |
T3356 |
conj |
diameter,shape |
R1869 |
T3359 |
T3356 |
prep |
of,shape |
R1870 |
T3360 |
T3361 |
det |
the,cells |
R1871 |
T3361 |
T3359 |
pobj |
cells,of |
R1872 |
T3362 |
T3361 |
nmod |
anxA7,cells |
R1873 |
T3363 |
T3362 |
punct |
-,anxA7 |
R1874 |
T3364 |
T3362 |
punct |
/,anxA7 |
R1875 |
T3365 |
T3362 |
punct |
-,anxA7 |
R1876 |
T3366 |
T3361 |
amod |
red,cells |
R1877 |
T3367 |
T3361 |
compound |
blood,cells |
R1878 |
T3368 |
T3333 |
punct |
.,revealed |
R1879 |
T3370 |
T3371 |
nsubj |
They,had |
R1880 |
T3372 |
T3373 |
det |
a,diameter |
R1902 |
T3394 |
T3389 |
nsubj |
n,40 |
R1903 |
T3395 |
T3389 |
punct |
=,40 |
R1904 |
T3396 |
T3389 |
punct |
),40 |
R1905 |
T3397 |
T3373 |
punct |
", ",diameter |
R1906 |
T3398 |
T3399 |
det |
a,impression |
R1907 |
T3399 |
T3373 |
conj |
impression,diameter |
R1908 |
T3400 |
T3401 |
advmod |
remarkably,lower |
R1909 |
T3401 |
T3399 |
amod |
lower,impression |
R1910 |
T3402 |
T3399 |
amod |
emphasized,impression |
R1911 |
T3403 |
T3399 |
amod |
central,impression |
R1912 |
T3404 |
T3399 |
cc |
and,impression |
R1913 |
T3405 |
T3406 |
det |
a,shape |
R1914 |
T3406 |
T3399 |
conj |
shape,impression |
R1915 |
T3407 |
T3408 |
advmod |
more,flat |
R1916 |
T3408 |
T3406 |
amod |
flat,shape |
R1917 |
T3409 |
T3410 |
punct |
(,Fig. |
R1918 |
T3410 |
T3406 |
parataxis |
Fig.,shape |
R1919 |
T3411 |
T3410 |
nummod |
4,Fig. |
R1920 |
T3412 |
T3410 |
punct |
),Fig. |
R1921 |
T3413 |
T3371 |
punct |
.,had |
R1922 |
T3415 |
T3416 |
det |
No,change |
R1923 |
T3416 |
T3418 |
nsubjpass |
change,measured |
R1924 |
T3417 |
T3416 |
amod |
significant,change |
R1925 |
T3419 |
T3416 |
prep |
in,change |
R1926 |
T3420 |
T3421 |
det |
the,volume |
R1927 |
T3421 |
T3419 |
pobj |
volume,in |
R1928 |
T3422 |
T3421 |
amod |
mean,volume |
R1929 |
T3423 |
T3421 |
amod |
corpuscular,volume |
R1930 |
T3424 |
T3421 |
punct |
(,volume |
R1931 |
T3425 |
T3421 |
appos |
MCV,volume |
R1932 |
T3426 |
T3418 |
punct |
),measured |
R1933 |
T3427 |
T3418 |
auxpass |
was,measured |
R1934 |
T3428 |
T3418 |
prep |
with,measured |
R1935 |
T3429 |
T3430 |
det |
the,counter |
R1936 |
T3430 |
T3428 |
pobj |
counter,with |
R1937 |
T3431 |
T3430 |
nmod |
ADVIA,counter |
R1938 |
T3432 |
T3431 |
nummod |
120,ADVIA |
R1939 |
T3433 |
T3430 |
compound |
cell,counter |
R1940 |
T3434 |
T3418 |
punct |
.,measured |
R1975 |
T3474 |
T3475 |
advmod |
where,play |
R1976 |
T3475 |
T3469 |
relcl |
play,cytoskeleton |
R1977 |
T3476 |
T3477 |
compound |
annexin,A7 |
R1978 |
T3477 |
T3475 |
nsubj |
A7,play |
R1979 |
T3478 |
T3475 |
aux |
might,play |
R1980 |
T3479 |
T3480 |
det |
a,role |
R1981 |
T3480 |
T3475 |
dobj |
role,play |
R1982 |
T3481 |
T3458 |
punct |
.,depends |
R1983 |
T3483 |
T3484 |
det |
The,resistance |
R1984 |
T3484 |
T3486 |
nsubj |
resistance,is |
R1985 |
T3485 |
T3484 |
amod |
osmotic,resistance |
R1986 |
T3487 |
T3484 |
punct |
", ",resistance |
R1987 |
T3488 |
T3489 |
dep |
which,is |
R1988 |
T3489 |
T3484 |
relcl |
is,resistance |
R1989 |
T3490 |
T3491 |
det |
the,resistance |
R1990 |
T3491 |
T3489 |
attr |
resistance,is |
R1991 |
T3492 |
T3491 |
prep |
towards,resistance |
R1992 |
T3493 |
T3492 |
pobj |
changes,towards |
R1993 |
T3494 |
T3489 |
prep |
in,is |
R1994 |
T3495 |
T3496 |
det |
the,strength |
R1995 |
T3496 |
T3494 |
pobj |
strength,in |
R1996 |
T3497 |
T3496 |
amod |
extracellular,strength |
R1997 |
T3498 |
T3496 |
amod |
ionic,strength |
R1998 |
T3499 |
T3486 |
punct |
", ",is |
R1999 |
T3500 |
T3501 |
det |
a,assay |
R2000 |
T3501 |
T3486 |
attr |
assay,is |
R2001 |
T3502 |
T3501 |
amod |
convenient,assay |
R2002 |
T3503 |
T3501 |
prep |
for,assay |
R2003 |
T3504 |
T3503 |
pobj |
analysis,for |
R2004 |
T3505 |
T3504 |
prep |
of,analysis |
R2005 |
T3506 |
T3507 |
det |
the,integrity |
R2006 |
T3507 |
T3505 |
pobj |
integrity,of |
R2007 |
T3508 |
T3509 |
amod |
red,cell |
R2008 |
T3509 |
T3507 |
compound |
cell,integrity |
R2009 |
T3510 |
T3509 |
compound |
blood,cell |
R2010 |
T3511 |
T3486 |
punct |
.,is |
R2011 |
T3513 |
T3514 |
nsubjpass |
It,measured |
R2012 |
T3515 |
T3514 |
auxpass |
is,measured |
R2013 |
T3516 |
T3514 |
prep |
as,measured |
R2014 |
T3517 |
T3518 |
det |
the,concentration |
R2015 |
T3518 |
T3516 |
pobj |
concentration,as |
R2016 |
T3519 |
T3520 |
compound |
sodium,chloride |
R2017 |
T3520 |
T3518 |
compound |
chloride,concentration |
R2018 |
T3521 |
T3522 |
prep |
at,starts |
R2019 |
T3522 |
T3518 |
relcl |
starts,concentration |
R2020 |
T3523 |
T3521 |
pobj |
which,at |
R2021 |
T3524 |
T3525 |
amod |
cellular,lysis |
R2022 |
T3525 |
T3522 |
nsubj |
lysis,starts |
R2023 |
T3526 |
T3518 |
punct |
(,concentration |
R2024 |
T3527 |
T3528 |
amod |
minimal,resistance |
R2025 |
T3528 |
T3518 |
appos |
resistance,concentration |
R2026 |
T3529 |
T3518 |
punct |
),concentration |
R2027 |
T3530 |
T3518 |
prep |
up,concentration |
R2028 |
T3531 |
T3530 |
prep |
to,up |
R2029 |
T3532 |
T3533 |
det |
a,lysis |
R2030 |
T3533 |
T3531 |
pobj |
lysis,to |
R2031 |
T3534 |
T3533 |
amod |
complete,lysis |
R2032 |
T3535 |
T3533 |
punct |
(,lysis |
R2033 |
T3536 |
T3537 |
amod |
maximal,resistance |
R2034 |
T3537 |
T3533 |
appos |
resistance,lysis |
R2035 |
T3538 |
T3533 |
punct |
),lysis |
R2036 |
T3539 |
T3533 |
prep |
of,lysis |
R2037 |
T3540 |
T3541 |
det |
the,cells |
R2038 |
T3541 |
T3539 |
pobj |
cells,of |
R2039 |
T3542 |
T3543 |
punct |
[,33 |
R2040 |
T3543 |
T3514 |
parataxis |
33,measured |
R2041 |
T3544 |
T3543 |
punct |
],33 |
R2042 |
T3545 |
T3514 |
punct |
.,measured |
R2043 |
T3547 |
T3548 |
amod |
Osmotic,resistance |
R2044 |
T3548 |
T3549 |
nsubj |
resistance,increases |
R2045 |
T3550 |
T3549 |
prep |
with,increases |
R2046 |
T3551 |
T3552 |
amod |
higher,MCV |
R2047 |
T3552 |
T3550 |
pobj |
MCV,with |
R2048 |
T3553 |
T3552 |
punct |
", ",MCV |
R2049 |
T3554 |
T3555 |
amod |
larger,area |
R2050 |
T3555 |
T3552 |
conj |
area,MCV |
R2051 |
T3556 |
T3555 |
compound |
surface,area |
R2052 |
T3557 |
T3555 |
cc |
and,area |
R2053 |
T3558 |
T3559 |
det |
a,degree |
R2054 |
T3559 |
T3555 |
conj |
degree,area |
R2055 |
T3560 |
T3559 |
amod |
higher,degree |
R2056 |
T3561 |
T3559 |
prep |
of,degree |
R2057 |
T3562 |
T3563 |
amod |
cellular,metabolism |
R2058 |
T3563 |
T3561 |
pobj |
metabolism,of |
R2059 |
T3564 |
T3552 |
acl |
stabilizing,MCV |
R2060 |
T3565 |
T3566 |
det |
the,levels |
R2061 |
T3566 |
T3564 |
dobj |
levels,stabilizing |
R2062 |
T3567 |
T3566 |
amod |
intracellular,levels |
R2063 |
T3568 |
T3566 |
compound |
ion,levels |
R2064 |
T3569 |
T3549 |
punct |
.,increases |
R2065 |
T3571 |
T3572 |
amod |
Aged,cells |
R2066 |
T3572 |
T3575 |
nsubj |
cells,show |
R2067 |
T3573 |
T3572 |
amod |
red,cells |
R2068 |
T3574 |
T3572 |
compound |
blood,cells |
R2069 |
T3576 |
T3577 |
det |
a,MCV |
R2070 |
T3577 |
T3575 |
dobj |
MCV,show |
R2071 |
T3578 |
T3577 |
amod |
lowered,MCV |
R2072 |
T3579 |
T3577 |
cc |
and,MCV |
R2073 |
T3580 |
T3581 |
amod |
lower,resistance |
R2074 |
T3581 |
T3577 |
conj |
resistance,MCV |
R2075 |
T3582 |
T3581 |
amod |
osmotic,resistance |
R2076 |
T3583 |
T3584 |
punct |
[,33 |
R2077 |
T3584 |
T3575 |
parataxis |
33,show |
R2078 |
T3585 |
T3584 |
punct |
],33 |
R2079 |
T3586 |
T3575 |
punct |
.,show |
R2080 |
T3588 |
T3589 |
amod |
Similar,properties |
R2081 |
T3589 |
T3590 |
nsubjpass |
properties,observed |
R2082 |
T3591 |
T3590 |
aux |
can,observed |
R2083 |
T3592 |
T3590 |
auxpass |
be,observed |
R2084 |
T3593 |
T3590 |
prep |
in,observed |
R2085 |
T3594 |
T3595 |
amod |
red,cells |
R2086 |
T3595 |
T3593 |
pobj |
cells,in |
R2087 |
T3596 |
T3595 |
compound |
blood,cells |
R2088 |
T3597 |
T3595 |
prep |
with,cells |
R2089 |
T3598 |
T3599 |
amod |
lowered,permeability |
R2090 |
T3599 |
T3597 |
pobj |
permeability,with |
R2091 |
T3600 |
T3599 |
compound |
membrane,permeability |
R2092 |
T3601 |
T3590 |
punct |
.,observed |
R2093 |
T3603 |
T3604 |
det |
The,resistance |
R2094 |
T3604 |
T3606 |
nsubj |
resistance,is |
R2095 |
T3605 |
T3604 |
amod |
osmotic,resistance |
R2096 |
T3607 |
T3604 |
prep |
of,resistance |
R2097 |
T3608 |
T3609 |
compound |
annexin,A7 |
R2098 |
T3609 |
T3610 |
npadvmod |
A7,deficient |
R2099 |
T3610 |
T3611 |
amod |
deficient,cells |
R2100 |
T3611 |
T3607 |
pobj |
cells,of |
R2101 |
T3612 |
T3611 |
amod |
red,cells |
R2102 |
T3613 |
T3611 |
compound |
blood,cells |
R2103 |
T3614 |
T3615 |
advmod |
significantly,increased |
R2104 |
T3615 |
T3606 |
acomp |
increased,is |
R2105 |
T3616 |
T3606 |
prep |
compared,is |
R2106 |
T3617 |
T3616 |
prep |
to,compared |
R2107 |
T3618 |
T3619 |
amod |
wild,type |
R2108 |
T3619 |
T3620 |
compound |
type,cells |
R2109 |
T3620 |
T3617 |
pobj |
cells,to |
R2110 |
T3621 |
T3606 |
punct |
.,is |
R2111 |
T3623 |
T3624 |
det |
The,value |
R2112 |
T3624 |
T3627 |
nsubjpass |
value,observed |
R2113 |
T3625 |
T3626 |
amod |
minimal,resistance |
R2114 |
T3626 |
T3624 |
compound |
resistance,value |
R2115 |
T3628 |
T3624 |
prep |
of,value |
R2116 |
T3629 |
T3630 |
compound |
knock,out |
R2117 |
T3630 |
T3631 |
compound |
out,cells |
R2118 |
T3631 |
T3628 |
pobj |
cells,of |
R2119 |
T3632 |
T3627 |
auxpass |
is,observed |
R2120 |
T3633 |
T3627 |
prep |
in,observed |
R2121 |
T3634 |
T3635 |
det |
a,solution |
R2122 |
T3635 |
T3633 |
pobj |
solution,in |
R2123 |
T3636 |
T3637 |
nummod |
0.60,% |
R2124 |
T3637 |
T3635 |
compound |
%,solution |
R2125 |
T3638 |
T3635 |
compound |
NaCl,solution |
R2126 |
T3639 |
T3635 |
cc |
versus,solution |
R2127 |
T3640 |
T3641 |
nummod |
0.65,% |
R2128 |
T3641 |
T3635 |
conj |
%,solution |
R2129 |
T3642 |
T3641 |
prep |
for,% |
R2130 |
T3643 |
T3644 |
amod |
wild,type |
R2131 |
T3644 |
T3645 |
compound |
type,cells |
R2132 |
T3645 |
T3642 |
pobj |
cells,for |
R2133 |
T3646 |
T3627 |
punct |
.,observed |
R2134 |
T3648 |
T3649 |
nummod |
50,% |
R2135 |
T3649 |
T3650 |
compound |
%,haemolysis |
R2136 |
T3650 |
T3651 |
nsubjpass |
haemolysis,achieved |
R2137 |
T3652 |
T3651 |
auxpass |
is,achieved |
R2138 |
T3653 |
T3651 |
prep |
at,achieved |
R2139 |
T3654 |
T3655 |
nummod |
0.516,% |
R2140 |
T3655 |
T3656 |
compound |
%,solution |
R2141 |
T3656 |
T3653 |
pobj |
solution,at |
R2142 |
T3657 |
T3656 |
compound |
NaCl,solution |
R2143 |
T3658 |
T3656 |
prep |
for,solution |
R2144 |
T3659 |
T3660 |
compound |
knock,out |
R2145 |
T3660 |
T3658 |
pobj |
out,for |
R2146 |
T3661 |
T3656 |
cc |
versus,solution |
R2147 |
T3662 |
T3663 |
nummod |
0.564,% |
R2148 |
T3663 |
T3664 |
compound |
%,NaCl |
R2149 |
T3664 |
T3656 |
conj |
NaCl,solution |
R2150 |
T3665 |
T3664 |
prep |
for,NaCl |
R2151 |
T3666 |
T3667 |
amod |
wild,type |
R2152 |
T3667 |
T3668 |
compound |
type,cells |
R2153 |
T3668 |
T3665 |
pobj |
cells,for |
R2154 |
T3669 |
T3670 |
punct |
(,Fig. |
R2155 |
T3670 |
T3651 |
parataxis |
Fig.,achieved |
R2156 |
T3671 |
T3672 |
compound |
mean,values |
R2157 |
T3672 |
T3670 |
dep |
values,Fig. |
R2158 |
T3673 |
T3670 |
punct |
", ",Fig. |
R2159 |
T3674 |
T3675 |
nsubj |
p,0.00066 |
R2160 |
T3675 |
T3670 |
ccomp |
0.00066,Fig. |
R2161 |
T3676 |
T3675 |
punct |
=,0.00066 |
R2162 |
T3677 |
T3670 |
punct |
", ",Fig. |
R2163 |
T3678 |
T3679 |
nsubj |
n,8 |
R2164 |
T3679 |
T3670 |
ccomp |
8,Fig. |
R2165 |
T3680 |
T3679 |
punct |
=,8 |
R2166 |
T3681 |
T3670 |
punct |
;,Fig. |
R2167 |
T3682 |
T3670 |
nummod |
5,Fig. |
R2168 |
T3683 |
T3670 |
punct |
),Fig. |
R2169 |
T3684 |
T3651 |
punct |
.,achieved |
R2170 |
T3686 |
T3687 |
det |
The,width |
R2171 |
T3687 |
T3689 |
nsubj |
width,is |
R2172 |
T3688 |
T3687 |
compound |
resistance,width |
R2173 |
T3690 |
T3687 |
prep |
of,width |
R2174 |
T3691 |
T3692 |
det |
the,cells |
R2175 |
T3692 |
T3690 |
pobj |
cells,of |
R2176 |
T3693 |
T3694 |
compound |
knock,out |
R2177 |
T3694 |
T3692 |
compound |
out,cells |
R2178 |
T3695 |
T3696 |
advmod |
slightly,lower |
R2179 |
T3696 |
T3689 |
acomp |
lower,is |
R2180 |
T3697 |
T3698 |
punct |
(,solution |
R2181 |
T3698 |
T3696 |
parataxis |
solution,lower |
R2182 |
T3699 |
T3700 |
nummod |
0.20,% |
R2183 |
T3700 |
T3701 |
nmod |
%,NaCl |
R2184 |
T3701 |
T3698 |
compound |
NaCl,solution |
R2185 |
T3702 |
T3703 |
advmod |
instead,of |
R2186 |
T3703 |
T3700 |
cc |
of,% |
R2187 |
T3704 |
T3705 |
nummod |
0.25,% |
R2188 |
T3705 |
T3700 |
conj |
%,% |
R2189 |
T3706 |
T3698 |
punct |
),solution |
R2190 |
T3707 |
T3689 |
punct |
.,is |
R2191 |
T3709 |
T3710 |
nsubj |
We,performed |
R2192 |
T3711 |
T3710 |
advmod |
also,performed |
R2193 |
T3712 |
T3713 |
amod |
direct,measurements |
R2194 |
T3713 |
T3710 |
dobj |
measurements,performed |
R2195 |
T3714 |
T3713 |
prep |
of,measurements |
R2196 |
T3715 |
T3716 |
det |
the,deformability |
R2197 |
T3716 |
T3714 |
pobj |
deformability,of |
R2198 |
T3717 |
T3718 |
amod |
red,cell |
R2199 |
T3718 |
T3716 |
compound |
cell,deformability |
R2200 |
T3719 |
T3718 |
compound |
blood,cell |
R2201 |
T3720 |
T3716 |
compound |
membrane,deformability |
R2202 |
T3721 |
T3710 |
punct |
", ",performed |
R2203 |
T3722 |
T3723 |
aux |
to,characterize |
R2204 |
T3723 |
T3710 |
advcl |
characterize,performed |
R2205 |
T3724 |
T3723 |
advmod |
further,characterize |
R2206 |
T3725 |
T3726 |
det |
a,role |
R2207 |
T3726 |
T3723 |
dobj |
role,characterize |
R2208 |
T3727 |
T3726 |
amod |
possible,role |
R2209 |
T3728 |
T3726 |
prep |
of,role |
R2210 |
T3729 |
T3730 |
compound |
annexin,A7 |
R2211 |
T3730 |
T3728 |
pobj |
A7,of |
R2212 |
T3731 |
T3726 |
prep |
on,role |
R2213 |
T3732 |
T3733 |
det |
the,stability |
R2214 |
T3733 |
T3731 |
pobj |
stability,on |
R2215 |
T3734 |
T3733 |
compound |
membrane,stability |
R2216 |
T3735 |
T3710 |
punct |
.,performed |
R2217 |
T3737 |
T3738 |
prep |
With,tried |
R2218 |
T3739 |
T3740 |
compound |
micropipette,experiments |
R2219 |
T3740 |
T3737 |
pobj |
experiments,With |
R2220 |
T3741 |
T3738 |
nsubj |
we,tried |
R2221 |
T3742 |
T3743 |
aux |
to,correlate |
R2222 |
T3743 |
T3738 |
xcomp |
correlate,tried |
R2223 |
T3744 |
T3745 |
amod |
mechanical,characteristics |
R2224 |
T3745 |
T3743 |
dobj |
characteristics,correlate |
R2225 |
T3746 |
T3745 |
prep |
of,characteristics |
R2226 |
T3747 |
T3748 |
det |
the,cells |
R2227 |
T3748 |
T3746 |
pobj |
cells,of |
R2228 |
T3749 |
T3748 |
amod |
red,cells |
R2229 |
T3750 |
T3748 |
compound |
blood,cells |
R2230 |
T3751 |
T3743 |
prep |
with,correlate |
R2250 |
T3773 |
T3774 |
det |
these,experiments |
R2251 |
T3774 |
T3772 |
pobj |
experiments,in |
R2252 |
T3775 |
T3776 |
punct |
(,M. |
R2253 |
T3776 |
T3757 |
meta |
M.,observe |
R2254 |
T3777 |
T3776 |
nmod |
Heil,M. |
R2255 |
T3778 |
T3776 |
punct |
", ",M. |
R2256 |
T3779 |
T3776 |
conj |
B.,M. |
R2257 |
T3780 |
T3779 |
nmod |
Hoffmann,B. |
R2258 |
T3781 |
T3779 |
cc |
and,B. |
R2259 |
T3782 |
T3779 |
conj |
R.,B. |
R2260 |
T3783 |
T3782 |
nmod |
Merkel,R. |
R2261 |
T3784 |
T3782 |
punct |
", ",R. |
R2262 |
T3785 |
T3786 |
amod |
unpublished,results |
R2263 |
T3786 |
T3782 |
conj |
results,R. |
R2264 |
T3787 |
T3786 |
punct |
),results |
R2265 |
T3788 |
T3757 |
punct |
.,observe |
R2266 |
T3790 |
T3791 |
advmod |
However,was |
R2267 |
T3792 |
T3791 |
punct |
", ",was |
R2268 |
T3793 |
T3794 |
det |
the,distinction |
R2269 |
T3794 |
T3791 |
nsubj |
distinction,was |
R2270 |
T3795 |
T3794 |
acl |
observed,distinction |
R2271 |
T3796 |
T3795 |
prep |
in,observed |
R2272 |
T3797 |
T3798 |
det |
the,experiments |
R2273 |
T3798 |
T3796 |
pobj |
experiments,in |
R2274 |
T3799 |
T3800 |
amod |
osmotic,resistance |
R2275 |
T3800 |
T3798 |
compound |
resistance,experiments |
R2276 |
T3801 |
T3802 |
advmod |
highly,significant |
R2277 |
T3802 |
T3791 |
acomp |
significant,was |
R2278 |
T3803 |
T3791 |
cc |
and,was |
R2279 |
T3804 |
T3791 |
conj |
reflects,was |
R2280 |
T3805 |
T3806 |
det |
a,value |
R2281 |
T3806 |
T3804 |
dobj |
value,reflects |
R2282 |
T3807 |
T3806 |
amod |
mean,value |
R2283 |
T3808 |
T3806 |
prep |
over,value |
R2284 |
T3809 |
T3810 |
det |
a,number |
R2285 |
T3810 |
T3808 |
pobj |
number,over |
R2286 |
T3811 |
T3810 |
amod |
high,number |
R2287 |
T3812 |
T3810 |
prep |
of,number |
R2288 |
T3813 |
T3814 |
amod |
different,cells |
R2289 |
T3814 |
T3812 |
pobj |
cells,of |
R2290 |
T3815 |
T3814 |
amod |
red,cells |
R2291 |
T3816 |
T3814 |
compound |
blood,cells |
R2292 |
T3817 |
T3791 |
punct |
.,was |
R2293 |
T3819 |
T3820 |
det |
These,data |
R2294 |
T3820 |
T3821 |
nsubj |
data,suggest |
R2295 |
T3822 |
T3823 |
mark |
that,contributes |
R2296 |
T3823 |
T3821 |
ccomp |
contributes,suggest |
R2297 |
T3824 |
T3825 |
compound |
annexin,A7 |
R2298 |
T3825 |
T3823 |
nsubj |
A7,contributes |
R2299 |
T3826 |
T3823 |
prep |
to,contributes |
R2300 |
T3827 |
T3828 |
det |
the,shape |
R2301 |
T3828 |
T3826 |
pobj |
shape,to |
R2302 |
T3829 |
T3828 |
prep |
of,shape |
R2303 |
T3830 |
T3831 |
amod |
red,cells |
R2304 |
T3831 |
T3829 |
pobj |
cells,of |
R2305 |
T3832 |
T3831 |
compound |
blood,cells |
R2306 |
T3833 |
T3828 |
cc |
and,shape |
R2307 |
T3834 |
T3835 |
det |
the,resistance |
R2308 |
T3835 |
T3828 |
conj |
resistance,shape |
R2309 |
T3836 |
T3835 |
amod |
osmotic,resistance |
R2310 |
T3837 |
T3821 |
punct |
.,suggest |
R2311 |
T3839 |
T3840 |
mark |
As,observed |
R2312 |
T3840 |
T3844 |
advcl |
observed,do |
R2313 |
T3841 |
T3840 |
nsubj |
we,observed |
R2314 |
T3842 |
T3840 |
aux |
have,observed |
R2315 |
T3843 |
T3840 |
neg |
not,observed |
R2316 |
T3845 |
T3846 |
det |
a,binding |
R2317 |
T3846 |
T3840 |
dobj |
binding,observed |
R2318 |
T3847 |
T3846 |
prep |
of,binding |
R2319 |
T3848 |
T3849 |
compound |
annexin,A7 |
R2320 |
T3849 |
T3847 |
pobj |
A7,of |
R2321 |
T3850 |
T3846 |
prep |
to,binding |
R2322 |
T3851 |
T3852 |
compound |
F,actin |
R2323 |
T3852 |
T3850 |
pobj |
actin,to |
R2324 |
T3853 |
T3852 |
punct |
-,actin |
R2325 |
T3854 |
T3855 |
punct |
(,shown |
R2326 |
T3855 |
T3840 |
parataxis |
shown,observed |
R2327 |
T3856 |
T3855 |
nsubj |
data,shown |
R2328 |
T3857 |
T3855 |
neg |
not,shown |
R2329 |
T3858 |
T3855 |
punct |
),shown |
R2330 |
T3859 |
T3844 |
nsubj |
it,do |
R2331 |
T3860 |
T3844 |
aux |
could,do |
R2332 |
T3861 |
T3844 |
advmod |
so,do |
R2333 |
T3862 |
T3863 |
preconj |
either,by |
R2334 |
T3863 |
T3844 |
prep |
by,do |
R2335 |
T3864 |
T3863 |
pobj |
alteration,by |
R2336 |
T3865 |
T3864 |
prep |
of,alteration |
R2337 |
T3866 |
T3867 |
det |
the,rigidity |
R2338 |
T3867 |
T3865 |
pobj |
rigidity,of |
R2339 |
T3868 |
T3867 |
compound |
membrane,rigidity |
R2340 |
T3869 |
T3863 |
cc |
and,by |
R2341 |
T3870 |
T3869 |
punct |
/,and |
R2342 |
T3871 |
T3869 |
cc |
or,and |
R2343 |
T3872 |
T3863 |
conj |
by,by |
R2344 |
T3873 |
T3872 |
pcomp |
affecting,by |
R2345 |
T3874 |
T3875 |
det |
the,homeostasis |
R2346 |
T3875 |
T3873 |
dobj |
homeostasis,affecting |
R2347 |
T3876 |
T3875 |
compound |
ion,homeostasis |
R2348 |
T3877 |
T3844 |
punct |
.,do |
R2349 |
T4078 |
T4079 |
det |
The,lack |
R2350 |
T4079 |
T4080 |
nsubj |
lack,affects |
R2351 |
T4081 |
T4079 |
prep |
of,lack |
R2352 |
T4082 |
T4083 |
compound |
annexin,A7 |
R2353 |
T4083 |
T4081 |
pobj |
A7,of |
R2354 |
T4084 |
T4085 |
amod |
primary,haemostasis |
R2355 |
T4085 |
T4080 |
dobj |
haemostasis,affects |
R2356 |
T4086 |
T4087 |
advmod |
ex,vivo |
R2357 |
T4087 |
T4080 |
advmod |
vivo,affects |
R2358 |
T4089 |
T4090 |
det |
The,use |
R2359 |
T4090 |
T4091 |
nsubj |
use,allowed |
R2360 |
T4092 |
T4090 |
prep |
of,use |
R2361 |
T4093 |
T4094 |
det |
an,counter |
R2362 |
T4094 |
T4092 |
pobj |
counter,of |
R2363 |
T4095 |
T4094 |
amod |
advanced,counter |
R2364 |
T4096 |
T4094 |
amod |
electronic,counter |
R2365 |
T4097 |
T4094 |
compound |
cell,counter |
R2366 |
T4098 |
T4094 |
punct |
/,counter |
R2367 |
T4099 |
T4100 |
compound |
flow,cytometer |
R2368 |
T4100 |
T4094 |
appos |
cytometer,counter |
R2369 |
T4101 |
T4094 |
punct |
(,counter |
R2370 |
T4102 |
T4094 |
appos |
ADVIA,counter |
R2371 |
T4103 |
T4102 |
nummod |
120,ADVIA |
R2372 |
T4104 |
T4091 |
punct |
),allowed |
R2373 |
T4105 |
T4106 |
nsubj |
us,screen |
R2374 |
T4106 |
T4091 |
ccomp |
screen,allowed |
R2375 |
T4107 |
T4106 |
aux |
to,screen |
R2376 |
T4108 |
T4109 |
amod |
other,parameters |
R2377 |
T4109 |
T4106 |
dobj |
parameters,screen |
R2378 |
T4110 |
T4111 |
advmod |
as,well |
R2379 |
T4111 |
T4106 |
advmod |
well,screen |
R2380 |
T4112 |
T4106 |
prep |
despite,screen |
R2381 |
T4113 |
T4112 |
pcomp |
of,despite |
R2382 |
T4114 |
T4115 |
det |
the,volume |
R2383 |
T4115 |
T4112 |
pobj |
volume,despite |
R2384 |
T4116 |
T4115 |
amod |
limited,volume |
R2385 |
T4117 |
T4115 |
amod |
murine,volume |
R2386 |
T4118 |
T4115 |
compound |
blood,volume |
R2387 |
T4119 |
T4091 |
punct |
.,allowed |
R2388 |
T4121 |
T4122 |
nsubj |
We,analysed |
R2389 |
T4123 |
T4122 |
dobj |
leukocytes,analysed |
R2390 |
T4124 |
T4123 |
punct |
", ",leukocytes |
R2391 |
T4125 |
T4126 |
amod |
red,cells |
R2392 |
T4126 |
T4123 |
conj |
cells,leukocytes |
R2393 |
T4127 |
T4126 |
compound |
blood,cells |
R2394 |
T4128 |
T4126 |
punct |
", ",cells |
R2395 |
T4129 |
T4130 |
compound |
haemoglobin,content |
R2396 |
T4130 |
T4126 |
conj |
content,cells |
R2397 |
T4131 |
T4130 |
punct |
", ",content |
R2398 |
T4132 |
T4130 |
conj |
haematocrit,content |
R2399 |
T4133 |
T4132 |
punct |
", ",haematocrit |
R2400 |
T4134 |
T4135 |
amod |
mean,volume |
R2401 |
T4135 |
T4132 |
conj |
volume,haematocrit |
R2402 |
T4136 |
T4135 |
amod |
cellular,volume |
R2403 |
T4137 |
T4135 |
punct |
", ",volume |
R2404 |
T4138 |
T4139 |
amod |
mean,haemoglobin |
R2405 |
T4139 |
T4135 |
conj |
haemoglobin,volume |
R2406 |
T4140 |
T4139 |
amod |
cellular,haemoglobin |
R2407 |
T4141 |
T4139 |
punct |
", ",haemoglobin |
R2408 |
T4142 |
T4143 |
amod |
mean,haemoglobin |
R2409 |
T4143 |
T4145 |
compound |
haemoglobin,concentration |
R2410 |
T4144 |
T4143 |
amod |
cellular,haemoglobin |
R2411 |
T4145 |
T4139 |
conj |
concentration,haemoglobin |
R2412 |
T4146 |
T4145 |
punct |
", ",concentration |
R2413 |
T4147 |
T4145 |
conj |
platelets,concentration |
R2414 |
T4148 |
T4147 |
punct |
", ",platelets |
R2415 |
T4149 |
T4147 |
conj |
neutrophiles,platelets |
R2416 |
T4150 |
T4149 |
punct |
", ",neutrophiles |
R2417 |
T4151 |
T4149 |
conj |
lymphocytes,neutrophiles |
R2418 |
T4152 |
T4151 |
punct |
", ",lymphocytes |
R2419 |
T4153 |
T4151 |
conj |
monocytes,lymphocytes |
R2420 |
T4154 |
T4153 |
punct |
", ",monocytes |
R2421 |
T4155 |
T4153 |
conj |
eosinophiles,monocytes |
R2422 |
T4156 |
T4155 |
cc |
and,eosinophiles |
R2423 |
T4157 |
T4155 |
conj |
basophiles,eosinophiles |
R2424 |
T4158 |
T4122 |
punct |
.,analysed |
R2425 |
T4160 |
T4161 |
prep |
In,found |
R2426 |
T4162 |
T4163 |
det |
these,tests |
R2427 |
T4163 |
T4160 |
pobj |
tests,In |
R2428 |
T4164 |
T4163 |
prep |
of,tests |
R2429 |
T4165 |
T4166 |
amod |
whole,blood |
R2430 |
T4166 |
T4164 |
pobj |
blood,of |
R2431 |
T4167 |
T4161 |
nsubj |
we,found |
R2432 |
T4168 |
T4161 |
advmod |
only,found |
R2433 |
T4169 |
T4170 |
det |
a,change |
R2434 |
T4170 |
T4161 |
dobj |
change,found |
R2435 |
T4171 |
T4170 |
prep |
in,change |
R2436 |
T4172 |
T4173 |
det |
the,numbers |
R2437 |
T4173 |
T4171 |
pobj |
numbers,in |
R2438 |
T4174 |
T4173 |
compound |
platelet,numbers |
R2439 |
T4175 |
T4161 |
punct |
", ",found |
R2440 |
T4176 |
T4177 |
mark |
whereas,affected |
R2441 |
T4177 |
T4161 |
advcl |
affected,found |
R2442 |
T4178 |
T4179 |
det |
all,parameters |
R2443 |
T4179 |
T4177 |
nsubjpass |
parameters,affected |
R2444 |
T4180 |
T4179 |
amod |
other,parameters |
R2445 |
T4181 |
T4179 |
amod |
haematological,parameters |
R2446 |
T4182 |
T4177 |
auxpass |
were,affected |
R2447 |
T4183 |
T4177 |
neg |
not,affected |
R2448 |
T4184 |
T4161 |
punct |
.,found |
R2449 |
T4186 |
T4187 |
det |
The,counts |
R2450 |
T4187 |
T4190 |
nsubjpass |
counts,determined |
R2451 |
T4188 |
T4187 |
amod |
mean,counts |
R2452 |
T4189 |
T4187 |
compound |
platelet,counts |
R2453 |
T4191 |
T4187 |
prep |
from,counts |
R2454 |
T4192 |
T4193 |
amod |
wild,type |
R2455 |
T4193 |
T4194 |
nmod |
type,mice |
R2456 |
T4194 |
T4191 |
pobj |
mice,from |
R2457 |
T4195 |
T4193 |
cc |
and,type |
R2458 |
T4196 |
T4193 |
conj |
anxA7,type |
R2459 |
T4197 |
T4196 |
punct |
-,anxA7 |
R2460 |
T4198 |
T4196 |
punct |
/,anxA7 |
R2461 |
T4199 |
T4196 |
punct |
-,anxA7 |
R2462 |
T4200 |
T4194 |
amod |
mutant,mice |
R2463 |
T4201 |
T4190 |
auxpass |
are,determined |
R2464 |
T4202 |
T4190 |
prep |
as,determined |
R2465 |
T4203 |
T4204 |
quantmod |
674,103 |
R2466 |
T4204 |
T4202 |
pobj |
103,as |
R2467 |
T4205 |
T4204 |
punct |
×,103 |
R2468 |
T4206 |
T4207 |
punct |
/,μl |
R2469 |
T4207 |
T4204 |
prep |
μl,103 |
R2470 |
T4208 |
T4204 |
cc |
and,103 |
R2471 |
T4209 |
T4210 |
quantmod |
774,103 |
R2472 |
T4210 |
T4204 |
conj |
103,103 |
R2473 |
T4211 |
T4210 |
punct |
×,103 |
R2474 |
T4212 |
T4213 |
punct |
/,μl |
R2475 |
T4213 |
T4210 |
prep |
μl,103 |
R2476 |
T4214 |
T4190 |
punct |
", ",determined |
R2477 |
T4215 |
T4190 |
advmod |
respectively,determined |
R2478 |
T4216 |
T4190 |
punct |
.,determined |
R2479 |
T4218 |
T4219 |
det |
The,counts |
R2480 |
T4219 |
T4221 |
nsubj |
counts,are |
R2481 |
T4220 |
T4219 |
compound |
platelet,counts |
R2482 |
T4222 |
T4219 |
prep |
in,counts |
R2483 |
T4223 |
T4224 |
compound |
knock,out |
R2484 |
T4224 |
T4225 |
compound |
out,mice |
R2485 |
T4225 |
T4222 |
pobj |
mice,in |
R2486 |
T4226 |
T4227 |
advmod |
significantly,higher |
R2487 |
T4227 |
T4221 |
acomp |
higher,are |
R2488 |
T4228 |
T4229 |
punct |
(,14 |
R2489 |
T4229 |
T4227 |
parataxis |
14,higher |
R2490 |
T4230 |
T4231 |
nsubj |
p,0.0275 |
R2491 |
T4231 |
T4229 |
ccomp |
0.0275,14 |
R2492 |
T4232 |
T4231 |
punct |
=,0.0275 |
R2493 |
T4233 |
T4229 |
punct |
;,14 |
R2494 |
T4234 |
T4235 |
compound |
n,out |
R2495 |
T4235 |
T4237 |
nsubj |
out,11 |
R2496 |
T4236 |
T4235 |
compound |
knock,out |
R2497 |
T4237 |
T4229 |
ccomp |
11,14 |
R2498 |
T4238 |
T4237 |
punct |
=,11 |
R2499 |
T4239 |
T4229 |
punct |
", ",14 |
R2500 |
T4240 |
T4241 |
nmod |
n,type |
R2501 |
T4241 |
T4229 |
nsubj |
type,14 |
R2502 |
T4242 |
T4241 |
amod |
wild,type |
R2503 |
T4243 |
T4229 |
punct |
=,14 |
R2504 |
T4244 |
T4229 |
punct |
),14 |
R2505 |
T4245 |
T4221 |
punct |
.,are |
R2506 |
T4247 |
T4248 |
det |
An,increase |
R2507 |
T4248 |
T4249 |
nsubj |
increase,is |
R2508 |
T4250 |
T4248 |
prep |
in,increase |
R2509 |
T4251 |
T4252 |
compound |
platelet,counts |
R2510 |
T4252 |
T4250 |
pobj |
counts,in |
R2511 |
T4253 |
T4254 |
det |
a,disorder |
R2512 |
T4254 |
T4249 |
attr |
disorder,is |
R2513 |
T4255 |
T4256 |
advmod |
rather,uncommon |
R2514 |
T4256 |
T4254 |
amod |
uncommon,disorder |
R2515 |
T4257 |
T4249 |
punct |
.,is |
R2516 |
T4259 |
T4260 |
prep |
In,increase |
R2517 |
T4261 |
T4259 |
pobj |
humans,In |
R2518 |
T4262 |
T4260 |
punct |
", ",increase |
R2519 |
T4263 |
T4264 |
compound |
platelet,counts |
R2520 |
T4264 |
T4260 |
nsubj |
counts,increase |
R2521 |
T4265 |
T4260 |
advmod |
normally,increase |
R2522 |
T4266 |
T4267 |
advmod |
only,transiently |
R2523 |
T4267 |
T4260 |
advmod |
transiently,increase |
R2524 |
T4268 |
T4260 |
prep |
as,increase |
R2525 |
T4269 |
T4268 |
pobj |
in,as |
R2526 |
T4270 |
T4271 |
amod |
reactive,thrombocytosis |
R2527 |
T4271 |
T4269 |
pobj |
thrombocytosis,in |
R2528 |
T4272 |
T4269 |
cc |
and,in |
R2529 |
T4273 |
T4269 |
conj |
under,in |
R2530 |
T4274 |
T4275 |
amod |
postoperative,conditions |
R2531 |
T4275 |
T4273 |
pobj |
conditions,under |
R2532 |
T4276 |
T4260 |
cc |
or,increase |
R2533 |
T4277 |
T4278 |
auxpass |
are,increased |
R2534 |
T4278 |
T4260 |
conj |
increased,increase |
R2535 |
T4279 |
T4278 |
advmod |
largely,increased |
R2536 |
T4280 |
T4278 |
prep |
in,increased |
R2537 |
T4281 |
T4282 |
amod |
neoplastic,diseases |
R2538 |
T4282 |
T4280 |
pobj |
diseases,in |
R2539 |
T4283 |
T4260 |
punct |
.,increase |
R2540 |
T4285 |
T4286 |
prep |
By,are |
R2541 |
T4287 |
T4285 |
pobj |
contrast,By |
R2542 |
T4288 |
T4286 |
punct |
", ",are |
R2543 |
T4289 |
T4290 |
det |
the,mice |
R2544 |
T4290 |
T4286 |
nsubj |
mice,are |
R2545 |
T4291 |
T4290 |
nmod |
anxA7,mice |
R2546 |
T4292 |
T4291 |
punct |
-,anxA7 |
R2547 |
T4293 |
T4291 |
punct |
/,anxA7 |
R2548 |
T4294 |
T4291 |
punct |
-,anxA7 |
R2549 |
T4295 |
T4286 |
acomp |
healthy,are |
R2550 |
T4296 |
T4295 |
cc |
and,healthy |
R2551 |
T4297 |
T4295 |
conj |
viable,healthy |
R2552 |
T4298 |
T4286 |
punct |
.,are |
R2553 |
T4300 |
T4301 |
advmod |
Therefore,tested |
R2554 |
T4302 |
T4301 |
nsubj |
we,tested |
R2555 |
T4303 |
T4304 |
det |
the,function |
R2556 |
T4304 |
T4301 |
dobj |
function,tested |
R2557 |
T4305 |
T4304 |
compound |
platelet,function |
R2558 |
T4306 |
T4301 |
cc |
and,tested |
R2559 |
T4307 |
T4301 |
conj |
performed,tested |
R2560 |
T4308 |
T4309 |
compound |
aggregometry,measurements |
R2561 |
T4309 |
T4307 |
dobj |
measurements,performed |
R2562 |
T4310 |
T4307 |
prep |
with,performed |
R2563 |
T4311 |
T4312 |
npadvmod |
platelet,rich |
R2564 |
T4312 |
T4313 |
amod |
rich,plasma |
R2565 |
T4313 |
T4310 |
pobj |
plasma,with |
R2566 |
T4314 |
T4301 |
punct |
.,tested |
R2567 |
T4316 |
T4317 |
compound |
Platelet,activation |
R2568 |
T4317 |
T4318 |
nsubjpass |
activation,observed |
R2569 |
T4319 |
T4318 |
auxpass |
is,observed |
R2570 |
T4320 |
T4318 |
prep |
as,observed |
R2571 |
T4321 |
T4322 |
det |
a,change |
R2572 |
T4322 |
T4320 |
pobj |
change,as |
R2573 |
T4323 |
T4322 |
amod |
morphological,change |
R2574 |
T4324 |
T4322 |
prep |
from,change |
R2575 |
T4325 |
T4326 |
det |
the,state |
R2576 |
T4326 |
T4324 |
pobj |
state,from |
R2577 |
T4327 |
T4326 |
amod |
resting,state |
R2578 |
T4328 |
T4326 |
amod |
discoid,state |
R2579 |
T4329 |
T4322 |
prep |
to,change |
R2580 |
T4330 |
T4331 |
amod |
activated,cells |
R2581 |
T4331 |
T4329 |
pobj |
cells,to |
R2582 |
T4332 |
T4331 |
amod |
spherical,cells |
R2583 |
T4333 |
T4331 |
prep |
with,cells |
R2584 |
T4334 |
T4333 |
pobj |
pseudopods,with |
R2585 |
T4335 |
T4318 |
punct |
.,observed |
R2586 |
T4337 |
T4338 |
det |
The,changes |
R2587 |
T4338 |
T4340 |
nsubj |
changes,are |
R2588 |
T4339 |
T4338 |
amod |
morphological,changes |
R2589 |
T4341 |
T4340 |
prep |
due,are |
R2590 |
T4342 |
T4341 |
pcomp |
to,due |
R2591 |
T4343 |
T4344 |
amod |
cytoskeletal,rearrangements |
R2592 |
T4344 |
T4341 |
pobj |
rearrangements,due |
R2593 |
T4345 |
T4340 |
punct |
.,are |
R2594 |
T4347 |
T4348 |
advmod |
In,vitro |
R2595 |
T4348 |
T4349 |
advmod |
vitro,form |
R2596 |
T4350 |
T4351 |
det |
the,cells |
R2597 |
T4351 |
T4349 |
nsubj |
cells,form |
R2598 |
T4352 |
T4351 |
amod |
activated,cells |
R2599 |
T4353 |
T4349 |
dobj |
aggregates,form |
R2600 |
T4354 |
T4349 |
advcl |
recruiting,form |
R2601 |
T4355 |
T4356 |
amod |
additional,cells |
R2602 |
T4356 |
T4354 |
dobj |
cells,recruiting |
R2603 |
T4357 |
T4354 |
prep |
in,recruiting |
R2604 |
T4358 |
T4359 |
det |
the,solution |
R2605 |
T4359 |
T4357 |
pobj |
solution,in |
R2606 |
T4360 |
T4361 |
advmod |
thereby,reducing |
R2607 |
T4361 |
T4354 |
dep |
reducing,recruiting |
R2608 |
T4362 |
T4363 |
poss |
its,cloudiness |
R2609 |
T4363 |
T4361 |
dobj |
cloudiness,reducing |
R2610 |
T4364 |
T4349 |
punct |
.,form |
R2611 |
T4366 |
T4367 |
nsubj |
Analysis,showed |
R2612 |
T4368 |
T4366 |
prep |
of,Analysis |
R2613 |
T4369 |
T4370 |
det |
the,values |
R2614 |
T4370 |
T4368 |
pobj |
values,of |
R2615 |
T4371 |
T4370 |
compound |
transmission,values |
R2616 |
T4372 |
T4370 |
prep |
of,values |
R2617 |
T4373 |
T4374 |
compound |
aggregation,curves |
R2618 |
T4374 |
T4372 |
pobj |
curves,of |
R2619 |
T4375 |
T4366 |
prep |
after,Analysis |
R2620 |
T4376 |
T4377 |
compound |
platelet,activation |
R2621 |
T4377 |
T4375 |
pobj |
activation,after |
R2622 |
T4378 |
T4377 |
prep |
by,activation |
R2623 |
T4379 |
T4380 |
compound |
ristocetin,addition |
R2624 |
T4380 |
T4378 |
pobj |
addition,by |
R2625 |
T4381 |
T4382 |
punct |
(,cofactor |
R2626 |
T4382 |
T4380 |
parataxis |
cofactor,addition |
R2627 |
T4383 |
T4384 |
compound |
von,Willebrand |
R2628 |
T4384 |
T4382 |
compound |
Willebrand,cofactor |
R2629 |
T4385 |
T4382 |
punct |
),cofactor |
R2630 |
T4386 |
T4387 |
mark |
that,differed |
R2631 |
T4387 |
T4367 |
ccomp |
differed,showed |
R2632 |
T4388 |
T4389 |
det |
both,curves |
R2633 |
T4389 |
T4387 |
nsubj |
curves,differed |
R2634 |
T4390 |
T4387 |
advmod |
significantly,differed |
R2635 |
T4391 |
T4387 |
prep |
at,differed |
R2636 |
T4392 |
T4393 |
det |
the,point |
R2637 |
T4393 |
T4391 |
pobj |
point,at |
R2638 |
T4394 |
T4393 |
compound |
time,point |
R2639 |
T4395 |
T4393 |
prep |
of,point |
R2640 |
T4396 |
T4397 |
nummod |
seven,seconds |
R2641 |
T4397 |
T4395 |
pobj |
seconds,of |
R2642 |
T4398 |
T4397 |
prep |
after,seconds |
R2643 |
T4399 |
T4400 |
compound |
platelet,initiation |
R2644 |
T4400 |
T4398 |
pobj |
initiation,after |
R2645 |
T4401 |
T4402 |
punct |
(,Fig. |
R2646 |
T4402 |
T4387 |
parataxis |
Fig.,differed |
R2647 |
T4403 |
T4404 |
nsubj |
p,0.0287 |
R2648 |
T4404 |
T4402 |
ccomp |
0.0287,Fig. |
R2649 |
T4405 |
T4404 |
punct |
=,0.0287 |
R2650 |
T4406 |
T4402 |
punct |
", ",Fig. |
R2651 |
T4407 |
T4408 |
compound |
n,out |
R2652 |
T4408 |
T4410 |
nsubj |
out,16 |
R2653 |
T4409 |
T4408 |
compound |
knock,out |
R2654 |
T4410 |
T4402 |
ccomp |
16,Fig. |
R2655 |
T4411 |
T4410 |
punct |
=,16 |
R2656 |
T4412 |
T4402 |
punct |
", ",Fig. |
R2657 |
T4413 |
T4414 |
nmod |
n,type |
R2658 |
T4414 |
T4416 |
nsubj |
type,15 |
R2659 |
T4415 |
T4414 |
amod |
wild,type |
R2660 |
T4416 |
T4402 |
ccomp |
15,Fig. |
R2661 |
T4417 |
T4416 |
punct |
=,15 |
R2662 |
T4418 |
T4402 |
punct |
;,Fig. |
R2663 |
T4419 |
T4402 |
nummod |
6,Fig. |
R2664 |
T4420 |
T4402 |
punct |
),Fig. |
R2665 |
T4421 |
T4367 |
punct |
.,showed |
R2666 |
T4423 |
T4424 |
compound |
Annexin,A7 |
R2667 |
T4424 |
T4425 |
npadvmod |
A7,deficient |
R2668 |
T4425 |
T4426 |
amod |
deficient,platelets |
R2669 |
T4426 |
T4427 |
nsubj |
platelets,showed |
R2670 |
T4428 |
T4429 |
det |
a,velocity |
R2671 |
T4429 |
T4427 |
dobj |
velocity,showed |
R2672 |
T4430 |
T4431 |
advmod |
slightly,lowered |
R2673 |
T4431 |
T4429 |
amod |
lowered,velocity |
R2674 |
T4432 |
T4429 |
compound |
aggregation,velocity |
R2675 |
T4433 |
T4427 |
punct |
.,showed |
R2676 |
T4435 |
T4436 |
advmod |
When,compared |
R2677 |
T4436 |
T4438 |
advcl |
compared,found |
R2678 |
T4437 |
T4436 |
nsubj |
we,compared |
R2679 |
T4439 |
T4440 |
det |
the,curves |
R2680 |
T4440 |
T4436 |
dobj |
curves,compared |
R2681 |
T4441 |
T4440 |
compound |
aggregation,curves |
R2682 |
T4442 |
T4440 |
prep |
of,curves |
R2683 |
T4443 |
T4444 |
amod |
human,platelets |
R2684 |
T4444 |
T4442 |
pobj |
platelets,of |
R2685 |
T4445 |
T4444 |
prep |
from,platelets |
R2686 |
T4446 |
T4447 |
det |
a,donor |
R2687 |
T4447 |
T4445 |
pobj |
donor,from |
R2688 |
T4448 |
T4447 |
amod |
healthy,donor |
R2689 |
T4449 |
T4436 |
prep |
with,compared |
R2690 |
T4450 |
T4451 |
det |
the,ones |
R2691 |
T4451 |
T4449 |
pobj |
ones,with |
R2692 |
T4452 |
T4451 |
acl |
obtained,ones |
R2693 |
T4453 |
T4452 |
prep |
from,obtained |
R2694 |
T4454 |
T4455 |
det |
an,individual |
R2695 |
T4455 |
T4453 |
pobj |
individual,from |
R2696 |
T4456 |
T4455 |
prep |
with,individual |
R2697 |
T4457 |
T4458 |
det |
a,defect |
R2698 |
T4458 |
T4456 |
pobj |
defect,with |
R2699 |
T4459 |
T4460 |
nmod |
von,Willebrand |
R2700 |
T4460 |
T4461 |
nmod |
Willebrand,factor |
R2701 |
T4461 |
T4458 |
nmod |
factor,defect |
R2702 |
T4462 |
T4458 |
nmod |
type,defect |
R2703 |
T4463 |
T4462 |
nummod |
1,type |
R2704 |
T4464 |
T4438 |
punct |
", ",found |
R2705 |
T4465 |
T4438 |
nsubj |
we,found |
R2706 |
T4466 |
T4467 |
mark |
that,was |
R2707 |
T4467 |
T4438 |
ccomp |
was,found |
R2708 |
T4468 |
T4469 |
det |
the,difference |
R2709 |
T4469 |
T4467 |
nsubj |
difference,was |
R2710 |
T4470 |
T4469 |
prep |
in,difference |
R2711 |
T4471 |
T4472 |
det |
the,curves |
R2712 |
T4472 |
T4470 |
pobj |
curves,in |
R2713 |
T4473 |
T4474 |
advmod |
much,more |
R2714 |
T4474 |
T4475 |
advmod |
more,pronounced |
R2715 |
T4475 |
T4467 |
acomp |
pronounced,was |
R2716 |
T4476 |
T4477 |
mark |
as,observed |
R2717 |
T4477 |
T4467 |
advcl |
observed,was |
R2718 |
T4478 |
T4477 |
prep |
in,observed |
R2719 |
T4479 |
T4480 |
poss |
our,studies |
R2720 |
T4480 |
T4478 |
pobj |
studies,in |
R2721 |
T4481 |
T4480 |
prep |
of,studies |
R2722 |
T4482 |
T4483 |
amod |
healthy,platelets |
R2723 |
T4483 |
T4481 |
pobj |
platelets,of |
R2724 |
T4484 |
T4483 |
compound |
mouse,platelets |
R2725 |
T4485 |
T4483 |
cc |
and,platelets |
R2726 |
T4486 |
T4487 |
nmod |
anxA7,platelets |
R2727 |
T4487 |
T4483 |
conj |
platelets,platelets |
R2728 |
T4488 |
T4486 |
punct |
-,anxA7 |
R2729 |
T4489 |
T4486 |
punct |
/,anxA7 |
R2730 |
T4490 |
T4486 |
punct |
-,anxA7 |
R2731 |
T4491 |
T4438 |
punct |
.,found |
R2732 |
T4493 |
T4494 |
advmod |
Furthermore,found |
R2733 |
T4495 |
T4494 |
nsubj |
we,found |
R2734 |
T4496 |
T4497 |
mark |
that,responded |
R2735 |
T4497 |
T4494 |
ccomp |
responded,found |
R2736 |
T4498 |
T4499 |
amod |
murine,platelets |
R2737 |
T4499 |
T4497 |
nsubj |
platelets,responded |
R2738 |
T4500 |
T4497 |
advmod |
immediately,responded |
R2739 |
T4501 |
T4497 |
prep |
to,responded |
R2740 |
T4502 |
T4503 |
det |
the,chemical |
R2741 |
T4503 |
T4501 |
pobj |
chemical,to |
R2742 |
T4504 |
T4503 |
amod |
initiating,chemical |
R2743 |
T4505 |
T4506 |
mark |
whereas,have |
R2744 |
T4506 |
T4497 |
advcl |
have,responded |
R2745 |
T4507 |
T4508 |
amod |
human,platelets |
R2746 |
T4508 |
T4506 |
nsubj |
platelets,have |
R2747 |
T4509 |
T4510 |
det |
a,phase |
R2748 |
T4510 |
T4506 |
dobj |
phase,have |
R2749 |
T4511 |
T4510 |
compound |
lag,phase |
R2750 |
T4512 |
T4513 |
punct |
[,23 |
R2751 |
T4513 |
T4494 |
parataxis |
23,found |
R2752 |
T4514 |
T4513 |
punct |
],23 |
R2753 |
T4515 |
T4494 |
punct |
.,found |
R2766 |
T5213 |
T5214 |
compound |
Annexin,A7 |
R2767 |
T5214 |
T5215 |
nsubjpass |
A7,thought |
R2768 |
T5216 |
T5215 |
auxpass |
is,thought |
R2769 |
T5217 |
T5218 |
aux |
to,have |
R2770 |
T5218 |
T5215 |
xcomp |
have,thought |
R2771 |
T5219 |
T5220 |
det |
a,role |
R2772 |
T5220 |
T5218 |
dobj |
role,have |
R2773 |
T5221 |
T5218 |
prep |
in,have |
R2774 |
T5222 |
T5223 |
compound |
vesicle,fusion |
R2775 |
T5223 |
T5221 |
pobj |
fusion,in |
R2776 |
T5224 |
T5221 |
cc |
and,in |
R2777 |
T5225 |
T5221 |
conj |
in,in |
R2778 |
T5226 |
T5225 |
pcomp |
regulating,in |
R2779 |
T5227 |
T5226 |
cc |
and,regulating |
R2780 |
T5228 |
T5226 |
conj |
stabilizing,regulating |
R2781 |
T5229 |
T5230 |
compound |
membrane,domains |
R2782 |
T5230 |
T5228 |
dobj |
domains,stabilizing |
R2783 |
T5231 |
T5232 |
punct |
[,1 |
R2784 |
T5232 |
T5215 |
parataxis |
1,thought |
R2785 |
T5233 |
T5232 |
punct |
],1 |
R2786 |
T5234 |
T5215 |
punct |
.,thought |
R2787 |
T5236 |
T5237 |
det |
The,protein |
R2788 |
T5237 |
T5238 |
nsubjpass |
protein,localized |
R2789 |
T5238 |
T5241 |
ccomp |
localized,is |
R2790 |
T5239 |
T5238 |
aux |
has,localized |
R2791 |
T5240 |
T5238 |
auxpass |
been,localized |
R2792 |
T5242 |
T5238 |
prep |
to,localized |
R2793 |
T5243 |
T5244 |
det |
the,membrane |
R2794 |
T5244 |
T5242 |
pobj |
membrane,to |
R2795 |
T5245 |
T5244 |
compound |
plasma,membrane |
R2796 |
T5246 |
T5244 |
prep |
including,membrane |
R2797 |
T5247 |
T5248 |
compound |
raft,subdomains |
R2798 |
T5248 |
T5246 |
pobj |
subdomains,including |
R2799 |
T5249 |
T5241 |
punct |
", ",is |
R2800 |
T5250 |
T5241 |
advmod |
furthermore,is |
R2801 |
T5251 |
T5241 |
nsubj |
it,is |
R2802 |
T5252 |
T5241 |
acomp |
present,is |
R2803 |
T5253 |
T5241 |
prep |
in,is |
R2804 |
T5254 |
T5255 |
det |
the,cytosol |
R2805 |
T5255 |
T5253 |
pobj |
cytosol,in |
R2806 |
T5256 |
T5257 |
advmod |
where,found |
R2807 |
T5257 |
T5255 |
relcl |
found,cytosol |
R2808 |
T5258 |
T5257 |
nsubjpass |
it,found |
R2809 |
T5259 |
T5257 |
auxpass |
is,found |
R2810 |
T5260 |
T5257 |
prep |
on,found |
R2811 |
T5261 |
T5262 |
amod |
subcellular,structures |
R2812 |
T5262 |
T5260 |
pobj |
structures,on |
R2813 |
T5263 |
T5264 |
npadvmod |
vesicle,like |
R2814 |
T5264 |
T5262 |
amod |
like,structures |
R2815 |
T5265 |
T5264 |
punct |
-,like |
R2816 |
T5266 |
T5241 |
punct |
.,is |
R2817 |
T5268 |
T5269 |
det |
This,distribution |
R2818 |
T5269 |
T5270 |
nsubj |
distribution,appears |
R2819 |
T5271 |
T5272 |
aux |
to,be |
R2820 |
T5272 |
T5270 |
xcomp |
be,appears |
R2821 |
T5273 |
T5272 |
acomp |
independent,be |
R2822 |
T5274 |
T5273 |
prep |
of,independent |
R2823 |
T5275 |
T5276 |
det |
the,type |
R2824 |
T5276 |
T5274 |
pobj |
type,of |
R2825 |
T5277 |
T5276 |
nmod |
tissue,type |
R2826 |
T5278 |
T5277 |
cc |
or,tissue |
R2827 |
T5279 |
T5277 |
conj |
cell,tissue |
R2828 |
T5280 |
T5276 |
acl |
analysed,type |
R2829 |
T5281 |
T5270 |
punct |
.,appears |
R2830 |
T5283 |
T5284 |
prep |
In,increase |
R2831 |
T5285 |
T5286 |
amod |
specialized,cells |
R2832 |
T5286 |
T5283 |
pobj |
cells,In |
R2833 |
T5287 |
T5285 |
cc |
or,specialized |
R2834 |
T5288 |
T5289 |
advmod |
terminally,differentiated |
R2835 |
T5289 |
T5285 |
conj |
differentiated,specialized |
R2836 |
T5290 |
T5284 |
punct |
", ",increase |
R2837 |
T5291 |
T5292 |
compound |
expression,levels |
R2838 |
T5292 |
T5284 |
nsubj |
levels,increase |
R2839 |
T5293 |
T5284 |
cc |
and,increase |
R2840 |
T5294 |
T5295 |
advmod |
frequently,expressed |
R2841 |
T5295 |
T5284 |
conj |
expressed,increase |
R2842 |
T5296 |
T5297 |
det |
the,larger |
R2843 |
T5297 |
T5295 |
nsubjpass |
larger,expressed |
R2844 |
T5298 |
T5297 |
prep |
of,larger |
R2845 |
T5299 |
T5300 |
det |
the,isoforms |
R2846 |
T5300 |
T5298 |
pobj |
isoforms,of |
R2847 |
T5301 |
T5300 |
nummod |
two,isoforms |
R2848 |
T5302 |
T5295 |
aux |
is,expressed |
R2849 |
T5303 |
T5295 |
auxpass |
being,expressed |
R2850 |
T5304 |
T5305 |
punct |
[,35 |
R2851 |
T5305 |
T5295 |
parataxis |
35,expressed |
R2852 |
T5306 |
T5305 |
nummod |
6,35 |
R2853 |
T5307 |
T5305 |
punct |
",",35 |
R2854 |
T5308 |
T5305 |
nummod |
34,35 |
R2855 |
T5309 |
T5305 |
punct |
",",35 |
R2856 |
T5310 |
T5305 |
punct |
],35 |
R2857 |
T5311 |
T5284 |
punct |
.,increase |
R2858 |
T5313 |
T5314 |
amod |
Red,cells |
R2859 |
T5314 |
T5316 |
nsubjpass |
cells,shown |
R2860 |
T5315 |
T5314 |
compound |
blood,cells |
R2861 |
T5317 |
T5314 |
punct |
", ",cells |
R2862 |
T5318 |
T5319 |
dep |
which,thought |
R2863 |
T5319 |
T5314 |
relcl |
thought,cells |
R2864 |
T5320 |
T5319 |
auxpass |
were,thought |
R2865 |
T5321 |
T5322 |
aux |
to,be |
R2866 |
T5322 |
T5319 |
xcomp |
be,thought |
R2867 |
T5323 |
T5322 |
acomp |
devoid,be |
R2868 |
T5324 |
T5323 |
prep |
of,devoid |
R2869 |
T5325 |
T5326 |
compound |
annexin,A7 |
R2870 |
T5326 |
T5324 |
pobj |
A7,of |
R2871 |
T5327 |
T5316 |
punct |
", ",shown |
R2872 |
T5328 |
T5316 |
auxpass |
were,shown |
R2873 |
T5329 |
T5316 |
advmod |
recently,shown |
R2874 |
T5330 |
T5331 |
aux |
to,harbor |
R2875 |
T5331 |
T5316 |
xcomp |
harbor,shown |
R2876 |
T5332 |
T5333 |
det |
the,isoform |
R2877 |
T5333 |
T5331 |
dobj |
isoform,harbor |
R2878 |
T5334 |
T5335 |
nummod |
47,kDa |
R2879 |
T5335 |
T5333 |
compound |
kDa,isoform |
R2880 |
T5336 |
T5337 |
advmod |
together,with |
R2881 |
T5337 |
T5333 |
prep |
with,isoform |
R2882 |
T5338 |
T5339 |
poss |
its,partner |
R2883 |
T5339 |
T5337 |
pobj |
partner,with |
R2884 |
T5340 |
T5339 |
amod |
binding,partner |
R2885 |
T5341 |
T5339 |
appos |
sorcin,partner |
R2886 |
T5342 |
T5343 |
punct |
[,7 |
R2887 |
T5343 |
T5316 |
parataxis |
7,shown |
R2888 |
T5344 |
T5343 |
punct |
],7 |
R2889 |
T5345 |
T5316 |
punct |
.,shown |
R2890 |
T5347 |
T5348 |
nsubj |
We,showed |
R2891 |
T5349 |
T5350 |
det |
the,presence |
R2892 |
T5350 |
T5348 |
dobj |
presence,showed |
R2893 |
T5351 |
T5350 |
prep |
of,presence |
R2894 |
T5352 |
T5353 |
det |
the,isoform |
R2895 |
T5353 |
T5351 |
pobj |
isoform,of |
R2896 |
T5354 |
T5355 |
nummod |
51,kDa |
R2897 |
T5355 |
T5353 |
compound |
kDa,isoform |
R2898 |
T5356 |
T5357 |
advmod |
as,well |
R2899 |
T5357 |
T5348 |
advmod |
well,showed |
R2900 |
T5358 |
T5348 |
punct |
", ",showed |
R2901 |
T5359 |
T5348 |
advcl |
adding,showed |
R2902 |
T5360 |
T5361 |
amod |
red,cells |
R2903 |
T5361 |
T5359 |
dobj |
cells,adding |
R2904 |
T5362 |
T5361 |
compound |
blood,cells |
R2905 |
T5363 |
T5359 |
prep |
to,adding |
R2906 |
T5364 |
T5365 |
det |
the,list |
R2907 |
T5365 |
T5363 |
pobj |
list,to |
R2908 |
T5366 |
T5365 |
prep |
of,list |
R2909 |
T5367 |
T5366 |
pobj |
cells,of |
R2910 |
T5368 |
T5369 |
dep |
that,harbor |
R2911 |
T5369 |
T5367 |
relcl |
harbor,cells |
R2912 |
T5370 |
T5371 |
det |
this,isoform |
R2913 |
T5371 |
T5369 |
dobj |
isoform,harbor |
R2914 |
T5372 |
T5348 |
punct |
.,showed |
R2915 |
T5374 |
T5375 |
ccomp |
Salzer,showed |
R2916 |
T5376 |
T5377 |
advmod |
et,al. |
R2917 |
T5377 |
T5374 |
advmod |
al.,Salzer |
R2918 |
T5378 |
T5379 |
punct |
[,7 |
R2919 |
T5379 |
T5374 |
parataxis |
7,Salzer |
R2920 |
T5380 |
T5379 |
punct |
],7 |
R2921 |
T5381 |
T5374 |
preconj |
not,Salzer |
R2922 |
T5382 |
T5381 |
advmod |
only,not |
R2923 |
T5383 |
T5374 |
conj |
demonstrated,Salzer |
R2924 |
T5384 |
T5385 |
det |
the,presence |
R2925 |
T5385 |
T5383 |
dobj |
presence,demonstrated |
R2926 |
T5386 |
T5385 |
prep |
of,presence |
R2927 |
T5387 |
T5388 |
compound |
annexin,A7 |
R2928 |
T5388 |
T5386 |
pobj |
A7,of |
R2929 |
T5389 |
T5383 |
prep |
in,demonstrated |
R2930 |
T5390 |
T5391 |
amod |
red,cells |
R2931 |
T5391 |
T5389 |
pobj |
cells,in |
R2932 |
T5392 |
T5391 |
compound |
blood,cells |
R2933 |
T5393 |
T5375 |
punct |
", ",showed |
R2934 |
T5394 |
T5375 |
nsubj |
they,showed |
R2935 |
T5395 |
T5375 |
advmod |
also,showed |
R2936 |
T5396 |
T5397 |
mark |
that,were |
R2937 |
T5397 |
T5375 |
ccomp |
were,showed |
R2938 |
T5398 |
T5399 |
compound |
annexin,A7 |
R2939 |
T5399 |
T5397 |
nsubj |
A7,were |
R2940 |
T5400 |
T5399 |
cc |
and,A7 |
R2941 |
T5401 |
T5399 |
conj |
sorcin,A7 |
R2942 |
T5402 |
T5397 |
acomp |
enriched,were |
R2943 |
T5403 |
T5397 |
prep |
in,were |
R2944 |
T5404 |
T5405 |
compound |
membrane,raft |
R2945 |
T5405 |
T5406 |
compound |
raft,domains |
R2946 |
T5406 |
T5403 |
pobj |
domains,in |
R2947 |
T5407 |
T5406 |
prep |
of,domains |
R2948 |
T5408 |
T5407 |
pobj |
nanovesicles,of |
R2949 |
T5409 |
T5408 |
acl |
formed,nanovesicles |
R2950 |
T5410 |
T5409 |
prep |
from,formed |
R2951 |
T5411 |
T5412 |
amod |
red,cells |
R2952 |
T5412 |
T5410 |
pobj |
cells,from |
R2953 |
T5413 |
T5412 |
compound |
blood,cells |
R2954 |
T5414 |
T5415 |
advmod |
in,vitro |
R2955 |
T5415 |
T5409 |
advmod |
vitro,formed |
R2956 |
T5416 |
T5375 |
punct |
.,showed |
R2957 |
T5418 |
T5419 |
compound |
Raft,domains |
R2958 |
T5419 |
T5420 |
nsubjpass |
domains,considered |
R2959 |
T5421 |
T5420 |
prep |
in,considered |
R2960 |
T5422 |
T5421 |
amod |
general,in |
R2961 |
T5423 |
T5420 |
auxpass |
are,considered |
R2962 |
T5424 |
T5425 |
amod |
key,players |
R2963 |
T5425 |
T5420 |
oprd |
players,considered |
R2964 |
T5426 |
T5425 |
prep |
in,players |
R2965 |
T5427 |
T5428 |
compound |
membrane,organization |
R2966 |
T5428 |
T5426 |
pobj |
organization,in |
R2967 |
T5429 |
T5426 |
cc |
and,in |
R2968 |
T5430 |
T5426 |
conj |
in,in |
R2969 |
T5431 |
T5430 |
pcomp |
mediating,in |
R2970 |
T5432 |
T5433 |
det |
the,process |
R2971 |
T5433 |
T5431 |
dobj |
process,mediating |
R2972 |
T5434 |
T5433 |
compound |
vesiculation,process |
R2973 |
T5435 |
T5420 |
punct |
.,considered |
R2974 |
T5437 |
T5438 |
nsubj |
They,result |
R2975 |
T5439 |
T5438 |
prep |
from,result |
R2976 |
T5440 |
T5441 |
det |
the,clustering |
R2977 |
T5441 |
T5439 |
pobj |
clustering,from |
R2978 |
T5442 |
T5441 |
prep |
of,clustering |
R2979 |
T5443 |
T5442 |
pobj |
sphingolipids,of |
R2980 |
T5444 |
T5443 |
cc |
and,sphingolipids |
R2981 |
T5445 |
T5443 |
conj |
cholesterol,sphingolipids |
R2982 |
T5446 |
T5441 |
prep |
in,clustering |
R2983 |
T5447 |
T5448 |
det |
the,leaflet |
R2984 |
T5448 |
T5446 |
pobj |
leaflet,in |
R2985 |
T5449 |
T5448 |
amod |
outer,leaflet |
R2986 |
T5450 |
T5448 |
prep |
of,leaflet |
R2987 |
T5451 |
T5452 |
det |
the,membrane |
R2988 |
T5452 |
T5450 |
pobj |
membrane,of |
R2989 |
T5453 |
T5452 |
acl |
connected,membrane |
R2990 |
T5454 |
T5453 |
prep |
to,connected |
R2991 |
T5455 |
T5454 |
pobj |
phospholipids,to |
R2992 |
T5456 |
T5455 |
cc |
and,phospholipids |
R2993 |
T5457 |
T5455 |
conj |
cholesterol,phospholipids |
R2994 |
T5458 |
T5453 |
prep |
in,connected |
R2995 |
T5459 |
T5460 |
det |
the,leaflet |
R2996 |
T5460 |
T5458 |
pobj |
leaflet,in |
R2997 |
T5461 |
T5460 |
amod |
inner,leaflet |
R2998 |
T5462 |
T5438 |
cc |
and,result |
R2999 |
T5463 |
T5438 |
conj |
are,result |
R3000 |
T5464 |
T5463 |
acomp |
enriched,are |
R3001 |
T5465 |
T5463 |
prep |
in,are |
R3002 |
T5466 |
T5467 |
amod |
special,proteins |
R3003 |
T5467 |
T5465 |
pobj |
proteins,in |
R3004 |
T5468 |
T5438 |
punct |
.,result |
R3005 |
T5470 |
T5471 |
advmod |
Biochemically,defined |
R3006 |
T5472 |
T5471 |
nsubjpass |
rafts,defined |
R3007 |
T5473 |
T5471 |
auxpass |
are,defined |
R3008 |
T5474 |
T5471 |
prep |
as,defined |
R3009 |
T5475 |
T5474 |
pobj |
membranes,as |
R3010 |
T5476 |
T5477 |
dep |
that,are |
R3011 |
T5477 |
T5475 |
relcl |
are,membranes |
R3012 |
T5478 |
T5477 |
acomp |
resistant,are |
R3013 |
T5479 |
T5478 |
prep |
to,resistant |
R3014 |
T5480 |
T5479 |
pobj |
extraction,to |
R3015 |
T5481 |
T5480 |
prep |
by,extraction |
R3016 |
T5482 |
T5483 |
amod |
cold,X |
R3017 |
T5483 |
T5481 |
pobj |
X,by |
R3018 |
T5484 |
T5483 |
compound |
Triton,X |
R3019 |
T5485 |
T5483 |
punct |
-,X |
R3020 |
T5486 |
T5483 |
nummod |
100,X |
R3021 |
T5487 |
T5471 |
cc |
and,defined |
R3022 |
T5488 |
T5489 |
aux |
can,floated |
R3023 |
T5489 |
T5471 |
conj |
floated,defined |
R3024 |
T5490 |
T5489 |
auxpass |
be,floated |
R3025 |
T5491 |
T5489 |
prep |
to,floated |
R3026 |
T5492 |
T5493 |
amod |
low,densities |
R3027 |
T5493 |
T5491 |
pobj |
densities,to |
R3028 |
T5494 |
T5489 |
prep |
in,floated |
R3029 |
T5495 |
T5496 |
compound |
sucrose,gradient |
R3030 |
T5496 |
T5497 |
compound |
gradient,centrifugation |
R3031 |
T5497 |
T5494 |
pobj |
centrifugation,in |
R3032 |
T5498 |
T5499 |
punct |
[,36 |
R3033 |
T5499 |
T5489 |
parataxis |
36,floated |
R3034 |
T5500 |
T5499 |
punct |
],36 |
R3035 |
T5501 |
T5471 |
punct |
.,defined |
R3036 |
T5503 |
T5504 |
det |
The,findings |
R3037 |
T5504 |
T5505 |
nsubj |
findings,suggested |
R3038 |
T5506 |
T5504 |
prep |
of,findings |
R3039 |
T5507 |
T5506 |
pobj |
Caohuy,of |
R3040 |
T5508 |
T5509 |
advmod |
et,al. |
R3041 |
T5509 |
T5507 |
advmod |
al.,Caohuy |
R3042 |
T5510 |
T5511 |
punct |
[,37 |
R3043 |
T5511 |
T5507 |
parataxis |
37,Caohuy |
R3044 |
T5512 |
T5511 |
punct |
],37 |
R3045 |
T5513 |
T5514 |
mark |
that,mediates |
R3046 |
T5514 |
T5504 |
acl |
mediates,findings |
R3047 |
T5515 |
T5516 |
compound |
annexin,A7 |
R3048 |
T5516 |
T5514 |
nsubj |
A7,mediates |
R3049 |
T5517 |
T5518 |
compound |
membrane,aggregation |
R3050 |
T5518 |
T5514 |
dobj |
aggregation,mediates |
R3051 |
T5519 |
T5505 |
advmod |
also,suggested |
R3052 |
T5520 |
T5521 |
mark |
that,is |
R3053 |
T5521 |
T5505 |
ccomp |
is,suggested |
R3054 |
T5522 |
T5523 |
compound |
annexin,A7 |
R3055 |
T5523 |
T5521 |
nsubj |
A7,is |
R3056 |
T5524 |
T5525 |
det |
an,component |
R3057 |
T5525 |
T5521 |
attr |
component,is |
R3058 |
T5526 |
T5525 |
amod |
essential,component |
R3059 |
T5527 |
T5521 |
prep |
during,is |
R3060 |
T5528 |
T5527 |
pobj |
vesiculation,during |
R3061 |
T5529 |
T5505 |
punct |
.,suggested |
R3062 |
T5531 |
T5532 |
prep |
In,reported |
R3063 |
T5533 |
T5531 |
pobj |
addition,In |
R3064 |
T5534 |
T5532 |
nsubj |
they,reported |
R3065 |
T5535 |
T5536 |
det |
a,activation |
R3066 |
T5536 |
T5532 |
dobj |
activation,reported |
R3067 |
T5537 |
T5536 |
amod |
further,activation |
R3068 |
T5538 |
T5536 |
prep |
of,activation |
R3069 |
T5539 |
T5540 |
det |
the,process |
R3070 |
T5540 |
T5538 |
pobj |
process,of |
R3071 |
T5541 |
T5540 |
compound |
vesiculation,process |
R3072 |
T5542 |
T5536 |
prep |
by,activation |
R3073 |
T5543 |
T5542 |
pobj |
GTP,by |
R3074 |
T5544 |
T5532 |
cc |
and,reported |
R3075 |
T5545 |
T5532 |
conj |
proposed,reported |
R3076 |
T5546 |
T5547 |
mark |
that,acts |
R3077 |
T5547 |
T5545 |
ccomp |
acts,proposed |
R3078 |
T5548 |
T5549 |
compound |
annexin,A7 |
R3079 |
T5549 |
T5547 |
nsubj |
A7,acts |
R3080 |
T5550 |
T5547 |
prep |
as,acts |
R3081 |
T5551 |
T5552 |
det |
a,GTPase |
R3082 |
T5552 |
T5550 |
pobj |
GTPase,as |
R3083 |
T5553 |
T5532 |
punct |
.,reported |
R3084 |
T5555 |
T5556 |
advmod |
Similarly,enhanced |
R3085 |
T5557 |
T5556 |
punct |
", ",enhanced |
R3086 |
T5558 |
T5559 |
det |
the,presence |
R3087 |
T5559 |
T5556 |
nsubj |
presence,enhanced |
R3088 |
T5560 |
T5559 |
prep |
of,presence |
R3089 |
T5561 |
T5560 |
pobj |
GTP,of |
R3090 |
T5562 |
T5563 |
det |
the,secretion |
R3091 |
T5563 |
T5556 |
dobj |
secretion,enhanced |
R3092 |
T5564 |
T5563 |
prep |
of,secretion |
R3093 |
T5565 |
T5564 |
pobj |
surfactant,of |
R3094 |
T5566 |
T5563 |
prep |
by,secretion |
R3095 |
T5567 |
T5568 |
compound |
annexin,A7 |
R3096 |
T5568 |
T5566 |
pobj |
A7,by |
R3097 |
T5569 |
T5556 |
punct |
.,enhanced |
R3098 |
T5571 |
T5572 |
det |
These,results |
R3099 |
T5572 |
T5573 |
nsubjpass |
results,thought |
R3100 |
T5574 |
T5573 |
auxpass |
were,thought |
R3101 |
T5575 |
T5576 |
aux |
to,support |
R3102 |
T5576 |
T5573 |
xcomp |
support,thought |
R3103 |
T5577 |
T5578 |
det |
a,role |
R3104 |
T5578 |
T5576 |
dobj |
role,support |
R3105 |
T5579 |
T5578 |
amod |
direct,role |
R3106 |
T5580 |
T5578 |
prep |
for,role |
R3107 |
T5581 |
T5582 |
compound |
annexin,A7 |
R3108 |
T5582 |
T5580 |
pobj |
A7,for |
R3109 |
T5583 |
T5578 |
prep |
in,role |
R3110 |
T5584 |
T5585 |
amod |
surfactant,secretion |
R3111 |
T5585 |
T5583 |
pobj |
secretion,in |
R3112 |
T5586 |
T5573 |
punct |
", ",thought |
R3113 |
T5587 |
T5573 |
cc |
but,thought |
R3114 |
T5588 |
T5589 |
prep |
in,separated |
R3115 |
T5589 |
T5573 |
conj |
separated,thought |
R3116 |
T5590 |
T5591 |
det |
these,studies |
R3117 |
T5591 |
T5588 |
pobj |
studies,in |
R3118 |
T5592 |
T5593 |
compound |
annexin,A7 |
R3119 |
T5593 |
T5594 |
npadvmod |
A7,mediated |
R3120 |
T5594 |
T5595 |
amod |
mediated,fusion |
R3121 |
T5595 |
T5589 |
nsubjpass |
fusion,separated |
R3122 |
T5596 |
T5595 |
compound |
membrane,fusion |
R3123 |
T5597 |
T5589 |
auxpass |
was,separated |
R3124 |
T5598 |
T5589 |
prep |
from,separated |
R3125 |
T5599 |
T5600 |
det |
a,mechanism |
R3126 |
T5600 |
T5598 |
pobj |
mechanism,from |
R3127 |
T5601 |
T5600 |
amod |
second,mechanism |
R3128 |
T5602 |
T5603 |
npadvmod |
GTP,dependent |
R3129 |
T5603 |
T5600 |
amod |
dependent,mechanism |
R3130 |
T5604 |
T5603 |
punct |
-,dependent |
R3131 |
T5605 |
T5606 |
punct |
[,38 |
R3132 |
T5606 |
T5589 |
parataxis |
38,separated |
R3133 |
T5607 |
T5606 |
punct |
],38 |
R3134 |
T5608 |
T5589 |
punct |
.,separated |
R3135 |
T5610 |
T5611 |
advcl |
Having,tested |
R3136 |
T5612 |
T5613 |
det |
an,mouse |
R3137 |
T5613 |
T5610 |
dobj |
mouse,Having |
R3138 |
T5614 |
T5615 |
compound |
annexin,A7 |
R3139 |
T5615 |
T5613 |
compound |
A7,mouse |
R3140 |
T5616 |
T5613 |
compound |
knockout,mouse |
R3141 |
T5617 |
T5611 |
nsubj |
we,tested |
R3142 |
T5618 |
T5619 |
det |
the,involvement |
R3143 |
T5619 |
T5611 |
dobj |
involvement,tested |
R3144 |
T5620 |
T5619 |
prep |
of,involvement |
R3145 |
T5621 |
T5622 |
compound |
annexin,A7 |
R3146 |
T5622 |
T5620 |
pobj |
A7,of |
R3147 |
T5623 |
T5619 |
prep |
in,involvement |
R3148 |
T5624 |
T5623 |
pobj |
budding,in |
R3149 |
T5625 |
T5624 |
prep |
of,budding |
R3150 |
T5626 |
T5627 |
amod |
red,cell |
R3151 |
T5627 |
T5629 |
compound |
cell,vesicles |
R3152 |
T5628 |
T5627 |
compound |
blood,cell |
R3153 |
T5629 |
T5625 |
pobj |
vesicles,of |
R3154 |
T5630 |
T5611 |
punct |
.,tested |
R3155 |
T5632 |
T5633 |
poss |
Our,data |
R3156 |
T5633 |
T5634 |
nsubj |
data,suggest |
R3157 |
T5635 |
T5636 |
mark |
that,is |
R3158 |
T5636 |
T5634 |
ccomp |
is,suggest |
R3159 |
T5637 |
T5638 |
det |
the,efficiency |
R3160 |
T5638 |
T5636 |
nsubj |
efficiency,is |
R3161 |
T5639 |
T5638 |
prep |
of,efficiency |
R3162 |
T5640 |
T5641 |
nmod |
micro,nanovesicle |
R3163 |
T5641 |
T5644 |
compound |
nanovesicle,formation |
R3164 |
T5642 |
T5641 |
punct |
-,nanovesicle |
R3165 |
T5643 |
T5641 |
cc |
and,nanovesicle |
R3166 |
T5644 |
T5639 |
pobj |
formation,of |
R3167 |
T5645 |
T5636 |
neg |
not,is |
R3168 |
T5646 |
T5647 |
advmod |
significantly,different |
R3169 |
T5647 |
T5636 |
acomp |
different,is |
R3170 |
T5648 |
T5636 |
prep |
in,is |
R3171 |
T5649 |
T5650 |
amod |
wild,type |
R3172 |
T5650 |
T5651 |
nmod |
type,cells |
R3173 |
T5651 |
T5648 |
pobj |
cells,in |
R3174 |
T5652 |
T5650 |
cc |
and,type |
R3175 |
T5653 |
T5650 |
conj |
mutant,type |
R3176 |
T5654 |
T5651 |
amod |
red,cells |
R3177 |
T5655 |
T5651 |
compound |
blood,cells |
R3178 |
T5656 |
T5634 |
punct |
.,suggest |
R3179 |
T5658 |
T5659 |
advmod |
Likewise,were |
R3180 |
T5660 |
T5659 |
punct |
", ",were |
R3181 |
T5661 |
T5662 |
compound |
Dictyostelium,discoideum |
R3182 |
T5662 |
T5663 |
compound |
discoideum,cells |
R3183 |
T5663 |
T5659 |
nsubj |
cells,were |
R3184 |
T5664 |
T5663 |
punct |
", ",cells |
R3185 |
T5665 |
T5666 |
prep |
in,was |
R3186 |
T5666 |
T5663 |
relcl |
was,cells |
R3187 |
T5667 |
T5665 |
pobj |
which,in |
R3188 |
T5668 |
T5669 |
det |
the,gene |
R3189 |
T5669 |
T5666 |
nsubj |
gene,was |
R3190 |
T5670 |
T5669 |
amod |
single,gene |
R3191 |
T5671 |
T5669 |
compound |
annexin,gene |
R3192 |
T5672 |
T5666 |
acomp |
inactivated,was |
R3193 |
T5673 |
T5659 |
punct |
", ",were |
R3194 |
T5674 |
T5659 |
neg |
not,were |
R3195 |
T5675 |
T5659 |
acomp |
impaired,were |
R3196 |
T5676 |
T5675 |
prep |
in,impaired |
R3197 |
T5677 |
T5676 |
pobj |
processes,in |
R3198 |
T5678 |
T5677 |
acl |
requiring,processes |
R3199 |
T5679 |
T5680 |
compound |
membrane,fusion |
R3200 |
T5680 |
T5678 |
dobj |
fusion,requiring |
R3201 |
T5681 |
T5682 |
punct |
[,39 |
R3202 |
T5682 |
T5659 |
parataxis |
39,were |
R3203 |
T5683 |
T5682 |
punct |
[,39 |
R3204 |
T5684 |
T5682 |
punct |
],39 |
R3205 |
T5685 |
T5682 |
punct |
;,39 |
R3206 |
T5686 |
T5682 |
cc |
and,39 |
R3207 |
T5687 |
T5682 |
conj |
data,39 |
R3208 |
T5688 |
T5687 |
amod |
unpublished,data |
R3209 |
T5689 |
T5687 |
punct |
],data |
R3210 |
T5690 |
T5659 |
punct |
.,were |
R3211 |
T5692 |
T5693 |
nsubj |
It,be |
R3212 |
T5694 |
T5693 |
aux |
might,be |
R3213 |
T5695 |
T5693 |
advmod |
well,be |
R3214 |
T5696 |
T5697 |
mark |
that,turn |
R3215 |
T5697 |
T5693 |
ccomp |
turn,be |
R3216 |
T5698 |
T5699 |
det |
the,fusion |
R3217 |
T5699 |
T5697 |
nsubj |
fusion,turn |
R3218 |
T5700 |
T5699 |
amod |
observed,fusion |
R3219 |
T5701 |
T5699 |
prep |
of,fusion |
R3220 |
T5702 |
T5703 |
compound |
phospholipid,layers |
R3221 |
T5703 |
T5701 |
pobj |
layers,of |
R3222 |
T5704 |
T5699 |
prep |
by,fusion |
R3223 |
T5705 |
T5706 |
compound |
annexin,A7 |
R3224 |
T5706 |
T5704 |
pobj |
A7,by |
R3225 |
T5707 |
T5699 |
prep |
in,fusion |
R3226 |
T5708 |
T5709 |
det |
the,presence |
R3227 |
T5709 |
T5707 |
pobj |
presence,in |
R3228 |
T5710 |
T5709 |
prep |
of,presence |
R3229 |
T5711 |
T5712 |
amod |
high,concentrations |
R3230 |
T5712 |
T5710 |
pobj |
concentrations,of |
R3231 |
T5713 |
T5712 |
compound |
Ca2+,concentrations |
R3232 |
T5714 |
T5697 |
aux |
may,turn |
R3233 |
T5715 |
T5697 |
prt |
out,turn |
R3234 |
T5716 |
T5717 |
aux |
to,be |
R3235 |
T5717 |
T5697 |
xcomp |
be,turn |
R3236 |
T5718 |
T5719 |
det |
an,effect |
R3237 |
T5719 |
T5717 |
attr |
effect,be |
R3238 |
T5720 |
T5721 |
advmod |
in,vitro |
R3239 |
T5721 |
T5719 |
amod |
vitro,effect |
R3240 |
T5722 |
T5693 |
punct |
.,be |
R3241 |
T5724 |
T5725 |
advmod |
Similarly,be |
R3242 |
T5726 |
T5725 |
punct |
", ",be |
R3243 |
T5727 |
T5728 |
det |
the,abundance |
R3244 |
T5728 |
T5725 |
nsubj |
abundance,be |
R3245 |
T5729 |
T5728 |
amod |
high,abundance |
R3246 |
T5730 |
T5728 |
prep |
of,abundance |
R3247 |
T5731 |
T5732 |
compound |
annexin,A7 |
R3248 |
T5732 |
T5730 |
pobj |
A7,of |
R3249 |
T5733 |
T5728 |
prep |
in,abundance |
R3250 |
T5734 |
T5735 |
nmod |
micro,nanovesicles |
R3251 |
T5735 |
T5733 |
pobj |
nanovesicles,in |
R3252 |
T5736 |
T5735 |
punct |
-,nanovesicles |
R3253 |
T5737 |
T5735 |
cc |
and,nanovesicles |
R3254 |
T5738 |
T5725 |
aux |
might,be |
R3255 |
T5739 |
T5725 |
acomp |
fortuitous,be |
R3256 |
T5740 |
T5741 |
mark |
as,appears |
R3257 |
T5741 |
T5725 |
advcl |
appears,be |
R3258 |
T5742 |
T5743 |
det |
a,percentage |
R3259 |
T5743 |
T5741 |
nsubj |
percentage,appears |
R3260 |
T5744 |
T5743 |
amod |
significant,percentage |
R3261 |
T5745 |
T5743 |
prep |
of,percentage |
R3262 |
T5746 |
T5747 |
det |
the,membrane |
R3263 |
T5747 |
T5745 |
pobj |
membrane,of |
R3264 |
T5748 |
T5749 |
amod |
red,cell |
R3265 |
T5749 |
T5747 |
compound |
cell,membrane |
R3266 |
T5750 |
T5749 |
compound |
blood,cell |
R3267 |
T5751 |
T5747 |
punct |
", ",membrane |
R3268 |
T5752 |
T5753 |
prep |
with,associates |
R3269 |
T5753 |
T5747 |
relcl |
associates,membrane |
R3270 |
T5754 |
T5752 |
pobj |
which,with |
R3271 |
T5755 |
T5756 |
compound |
annexin,A7 |
R3272 |
T5756 |
T5753 |
nsubj |
A7,associates |
R3273 |
T5757 |
T5758 |
advmod |
when,rise |
R3274 |
T5758 |
T5753 |
advcl |
rise,associates |
R3275 |
T5759 |
T5760 |
det |
the,levels |
R3276 |
T5760 |
T5758 |
nsubj |
levels,rise |
R3277 |
T5761 |
T5760 |
compound |
Ca2+,levels |
R3278 |
T5762 |
T5741 |
punct |
", ",appears |
R3279 |
T5763 |
T5764 |
aux |
to,involved |
R3280 |
T5764 |
T5741 |
xcomp |
involved,appears |
R3281 |
T5765 |
T5764 |
auxpass |
be,involved |
R3282 |
T5766 |
T5764 |
prep |
in,involved |
R3283 |
T5767 |
T5768 |
det |
the,formation |
R3284 |
T5768 |
T5766 |
pobj |
formation,in |
R3285 |
T5769 |
T5768 |
prep |
of,formation |
R3286 |
T5770 |
T5769 |
pobj |
exovesicles,of |
R3287 |
T5771 |
T5725 |
punct |
.,be |
R3288 |
T5773 |
T5774 |
advmod |
Furthermore,indicates |
R3289 |
T5775 |
T5774 |
punct |
", ",indicates |
R3290 |
T5776 |
T5777 |
det |
the,distribution |
R3291 |
T5777 |
T5774 |
nsubj |
distribution,indicates |
R3292 |
T5778 |
T5777 |
prep |
of,distribution |
R3293 |
T5779 |
T5780 |
det |
both,isoforms |
R3294 |
T5780 |
T5778 |
pobj |
isoforms,of |
R3295 |
T5781 |
T5782 |
compound |
annexin,A7 |
R3296 |
T5782 |
T5780 |
compound |
A7,isoforms |
R3297 |
T5783 |
T5777 |
prep |
in,distribution |
R3298 |
T5784 |
T5783 |
pobj |
gradients,in |
R3299 |
T5785 |
T5784 |
prep |
of,gradients |
R3300 |
T5786 |
T5787 |
amod |
red,cells |
R3301 |
T5787 |
T5785 |
pobj |
cells,of |
R3302 |
T5788 |
T5787 |
compound |
blood,cells |
R3303 |
T5789 |
T5790 |
det |
a,function |
R3304 |
T5790 |
T5774 |
dobj |
function,indicates |
R3305 |
T5791 |
T5792 |
dep |
which,differs |
R3306 |
T5792 |
T5790 |
relcl |
differs,function |
R3307 |
T5793 |
T5792 |
prep |
from,differs |
R3308 |
T5794 |
T5793 |
pcomp |
organizing,from |
R3309 |
T5795 |
T5796 |
amod |
subcellular,membranes |
R3310 |
T5796 |
T5794 |
dobj |
membranes,organizing |
R3311 |
T5797 |
T5796 |
cc |
or,membranes |
R3312 |
T5798 |
T5799 |
compound |
membrane,pathways |
R3313 |
T5799 |
T5796 |
conj |
pathways,membranes |
R3314 |
T5800 |
T5774 |
punct |
.,indicates |
R3315 |
T5802 |
T5803 |
mark |
Whereas,associated |
R3316 |
T5803 |
T5808 |
advcl |
associated,distributes |
R3317 |
T5804 |
T5805 |
compound |
annexin,A2 |
R3318 |
T5805 |
T5803 |
nsubjpass |
A2,associated |
R3319 |
T5806 |
T5803 |
auxpass |
is,associated |
R3320 |
T5807 |
T5803 |
advmod |
tightly,associated |
R3321 |
T5809 |
T5803 |
prep |
with,associated |
R3322 |
T5810 |
T5809 |
pobj |
endosomes,with |
R3323 |
T5811 |
T5803 |
cc |
and,associated |
R3324 |
T5812 |
T5813 |
aux |
may,have |
R3325 |
T5813 |
T5803 |
conj |
have,associated |
R3326 |
T5814 |
T5813 |
dobj |
functions,have |
R3327 |
T5815 |
T5813 |
prep |
in,have |
R3328 |
T5816 |
T5817 |
compound |
membrane,fusion |
R3329 |
T5817 |
T5815 |
pobj |
fusion,in |
R3330 |
T5818 |
T5817 |
cc |
and,fusion |
R3331 |
T5819 |
T5820 |
det |
the,pathway |
R3332 |
T5820 |
T5817 |
conj |
pathway,fusion |
R3333 |
T5821 |
T5820 |
amod |
endosomal,pathway |
R3334 |
T5822 |
T5808 |
punct |
", ",distributes |
R3335 |
T5823 |
T5824 |
compound |
annexin,A7 |
R3336 |
T5824 |
T5808 |
nsubj |
A7,distributes |
R3337 |
T5825 |
T5808 |
prep |
with,distributes |
R3338 |
T5826 |
T5827 |
compound |
plasma,membrane |
R3339 |
T5827 |
T5828 |
compound |
membrane,domains |
R3340 |
T5828 |
T5825 |
pobj |
domains,with |
R3341 |
T5829 |
T5828 |
compound |
raft,domains |
R3342 |
T5830 |
T5828 |
prep |
of,domains |
R3343 |
T5831 |
T5832 |
amod |
different,types |
R3344 |
T5832 |
T5830 |
pobj |
types,of |
R3345 |
T5833 |
T5832 |
cc |
and,types |
R3346 |
T5834 |
T5832 |
conj |
densities,types |
R3347 |
T5835 |
T5808 |
punct |
.,distributes |
R3348 |
T5837 |
T5838 |
advmod |
Interestingly,lowered |
R3349 |
T5839 |
T5838 |
punct |
", ",lowered |
R3350 |
T5840 |
T5838 |
prep |
in,lowered |
R3351 |
T5841 |
T5840 |
pobj |
vesicles,in |
R3352 |
T5842 |
T5841 |
prep |
of,vesicles |
R3353 |
T5843 |
T5844 |
amod |
red,cells |
R3354 |
T5844 |
T5842 |
pobj |
cells,of |
R3355 |
T5845 |
T5844 |
compound |
blood,cells |
R3356 |
T5846 |
T5844 |
acl |
lacking,cells |
R3357 |
T5847 |
T5848 |
compound |
annexin,A7 |
R3358 |
T5848 |
T5846 |
dobj |
A7,lacking |
R3359 |
T5849 |
T5850 |
det |
the,amount |
R3360 |
T5850 |
T5838 |
nsubjpass |
amount,lowered |
R3361 |
T5851 |
T5850 |
prep |
of,amount |
R3362 |
T5852 |
T5851 |
pobj |
sorcin,of |
R3363 |
T5853 |
T5838 |
auxpass |
is,lowered |
R3364 |
T5854 |
T5838 |
punct |
", ",lowered |
R3365 |
T5855 |
T5838 |
advcl |
indicating,lowered |
R3366 |
T5856 |
T5857 |
mark |
that,affected |
R3367 |
T5857 |
T5855 |
ccomp |
affected,indicating |
R3368 |
T5858 |
T5859 |
poss |
its,localisation |
R3369 |
T5859 |
T5857 |
nsubjpass |
localisation,affected |
R3370 |
T5860 |
T5857 |
auxpass |
is,affected |
R3371 |
T5861 |
T5857 |
agent |
by,affected |
R3372 |
T5862 |
T5863 |
det |
the,loss |
R3373 |
T5863 |
T5861 |
pobj |
loss,by |
R3374 |
T5864 |
T5863 |
prep |
of,loss |
R3375 |
T5865 |
T5866 |
compound |
annexin,A7 |
R3376 |
T5866 |
T5864 |
pobj |
A7,of |
R3377 |
T5867 |
T5857 |
cc |
or,affected |
R3378 |
T5868 |
T5869 |
poss |
its,expression |
R3379 |
T5869 |
T5870 |
nsubjpass |
expression,reduced |
R3380 |
T5870 |
T5857 |
conj |
reduced,affected |
R3381 |
T5871 |
T5869 |
prep |
during,expression |
R3382 |
T5872 |
T5873 |
amod |
red,cell |
R3383 |
T5873 |
T5875 |
compound |
cell,development |
R3384 |
T5874 |
T5873 |
compound |
blood,cell |
R3385 |
T5875 |
T5871 |
pobj |
development,during |
R3386 |
T5876 |
T5870 |
auxpass |
was,reduced |
R3387 |
T5877 |
T5838 |
punct |
.,lowered |
R3388 |
T5879 |
T5880 |
aux |
Having,excluded |
R3389 |
T5880 |
T5881 |
advcl |
excluded,act |
R3390 |
T5882 |
T5883 |
det |
a,role |
R3391 |
T5883 |
T5880 |
dobj |
role,excluded |
R3392 |
T5884 |
T5883 |
amod |
direct,role |
R3393 |
T5885 |
T5883 |
prep |
in,role |
R3394 |
T5886 |
T5887 |
compound |
vesicle,formation |
R3395 |
T5887 |
T5885 |
pobj |
formation,in |
R3396 |
T5888 |
T5887 |
cc |
and,formation |
R3397 |
T5889 |
T5890 |
compound |
membrane,fusion |
R3398 |
T5890 |
T5887 |
conj |
fusion,formation |
R3399 |
T5891 |
T5881 |
punct |
", ",act |
R3400 |
T5892 |
T5893 |
compound |
annexin,A7 |
R3401 |
T5893 |
T5881 |
nsubj |
A7,act |
R3402 |
T5894 |
T5881 |
aux |
might,act |
R3403 |
T5895 |
T5881 |
prep |
by,act |
R3404 |
T5896 |
T5897 |
poss |
its,property |
R3405 |
T5897 |
T5895 |
pobj |
property,by |
R3406 |
T5898 |
T5897 |
prep |
as,property |
R3407 |
T5899 |
T5900 |
npadvmod |
Ca2+,binding |
R3408 |
T5900 |
T5902 |
amod |
binding,protein |
R3409 |
T5901 |
T5900 |
punct |
-,binding |
R3410 |
T5902 |
T5898 |
pobj |
protein,as |
R3411 |
T5903 |
T5904 |
punct |
[,17 |
R3412 |
T5904 |
T5881 |
parataxis |
17,act |
R3413 |
T5905 |
T5904 |
punct |
],17 |
R3414 |
T5906 |
T5881 |
punct |
.,act |
R3415 |
T5908 |
T5909 |
amod |
Previous,results |
R3416 |
T5909 |
T5910 |
nsubjpass |
results,explained |
R3417 |
T5911 |
T5912 |
prep |
in,shown |
R3418 |
T5912 |
T5909 |
relcl |
shown,results |
R3419 |
T5913 |
T5911 |
pobj |
which,in |
R3420 |
T5914 |
T5915 |
compound |
annexin,A7 |
R3421 |
T5915 |
T5912 |
nsubjpass |
A7,shown |
R3422 |
T5916 |
T5912 |
auxpass |
was,shown |
R3423 |
T5917 |
T5918 |
aux |
to,play |
R3424 |
T5918 |
T5912 |
xcomp |
play,shown |
R3425 |
T5919 |
T5920 |
det |
a,role |
R3426 |
T5920 |
T5918 |
dobj |
role,play |
R3427 |
T5921 |
T5918 |
prep |
in,play |
R3428 |
T5922 |
T5923 |
compound |
membrane,aggregation |
R3429 |
T5923 |
T5921 |
pobj |
aggregation,in |
R3430 |
T5924 |
T5910 |
aux |
might,explained |
R3431 |
T5925 |
T5910 |
auxpass |
be,explained |
R3432 |
T5926 |
T5910 |
agent |
by,explained |
R3433 |
T5927 |
T5928 |
det |
a,function |
R3434 |
T5928 |
T5926 |
pobj |
function,by |
R3435 |
T5929 |
T5928 |
amod |
supportive,function |
R3436 |
T5930 |
T5928 |
prep |
of,function |
R3437 |
T5931 |
T5932 |
compound |
annexin,A7 |
R3438 |
T5932 |
T5930 |
pobj |
A7,of |
R3439 |
T5933 |
T5934 |
mark |
in,interferes |
R3440 |
T5934 |
T5910 |
advcl |
interferes,explained |
R3441 |
T5935 |
T5934 |
mark |
that,interferes |
R3442 |
T5936 |
T5934 |
nsubj |
it,interferes |
R3443 |
T5937 |
T5934 |
prep |
with,interferes |
R3444 |
T5938 |
T5939 |
det |
the,homeostasis |
R3445 |
T5939 |
T5937 |
pobj |
homeostasis,with |
R3446 |
T5940 |
T5939 |
amod |
local,homeostasis |
R3447 |
T5941 |
T5939 |
compound |
Ca2+,homeostasis |
R3448 |
T5942 |
T5943 |
advmod |
thus,influencing |
R3449 |
T5943 |
T5934 |
advcl |
influencing,interferes |
R3450 |
T5944 |
T5945 |
compound |
membrane,organization |
R3451 |
T5945 |
T5943 |
dobj |
organization,influencing |
R3452 |
T5946 |
T5910 |
punct |
.,explained |
R3453 |
T5948 |
T5949 |
mark |
As,belong |
R3454 |
T5949 |
T5952 |
advcl |
belong,expected |
R3455 |
T5950 |
T5951 |
det |
the,annexins |
R3456 |
T5951 |
T5949 |
nsubj |
annexins,belong |
R3457 |
T5953 |
T5949 |
prep |
to,belong |
R3458 |
T5954 |
T5955 |
det |
a,family |
R3459 |
T5955 |
T5953 |
pobj |
family,to |
R3460 |
T5956 |
T5957 |
advmod |
wide,spread |
R3461 |
T5957 |
T5955 |
amod |
spread,family |
R3462 |
T5958 |
T5957 |
cc |
and,spread |
R3463 |
T5959 |
T5960 |
advmod |
evolutionarily,conserved |
R3464 |
T5960 |
T5957 |
conj |
conserved,spread |
R3465 |
T5961 |
T5955 |
compound |
protein,family |
R3466 |
T5962 |
T5952 |
punct |
", ",expected |
R3467 |
T5963 |
T5964 |
npadvmod |
redundant,identical |
R3468 |
T5964 |
T5968 |
amod |
identical,functions |
R3469 |
T5965 |
T5964 |
punct |
", ",identical |
R3470 |
T5966 |
T5964 |
cc |
but,identical |
R3471 |
T5967 |
T5966 |
neg |
not,but |
R3472 |
T5968 |
T5952 |
nsubjpass |
functions,expected |
R3473 |
T5969 |
T5952 |
auxpass |
are,expected |
R3474 |
T5970 |
T5952 |
punct |
.,expected |
R3475 |
T5972 |
T5973 |
advmod |
However,described |
R3476 |
T5974 |
T5973 |
punct |
", ",described |
R3477 |
T5975 |
T5976 |
advmod |
so,far |
R3478 |
T5976 |
T5973 |
advmod |
far,described |
R3479 |
T5977 |
T5978 |
det |
no,members |
R3480 |
T5978 |
T5973 |
nsubjpass |
members,described |
R3481 |
T5979 |
T5978 |
amod |
other,members |
R3482 |
T5980 |
T5978 |
prep |
of,members |
R3483 |
T5981 |
T5982 |
det |
the,family |
R3484 |
T5982 |
T5980 |
pobj |
family,of |
R3485 |
T5983 |
T5982 |
compound |
annexin,family |
R3486 |
T5984 |
T5982 |
punct |
", ",family |
R3487 |
T5985 |
T5986 |
dep |
which,substitute |
R3488 |
T5986 |
T5982 |
relcl |
substitute,family |
R3489 |
T5987 |
T5986 |
aux |
may,substitute |
R3490 |
T5988 |
T5986 |
prep |
for,substitute |
R3491 |
T5989 |
T5990 |
compound |
annexin,A7 |
R3492 |
T5990 |
T5988 |
pobj |
A7,for |
R3493 |
T5991 |
T5973 |
punct |
", ",described |
R3494 |
T5992 |
T5973 |
aux |
have,described |
R3495 |
T5993 |
T5973 |
auxpass |
been,described |
R3496 |
T5994 |
T5973 |
prep |
in,described |
R3497 |
T5995 |
T5996 |
amod |
red,cells |
R3498 |
T5996 |
T5994 |
pobj |
cells,in |
R3499 |
T5997 |
T5996 |
compound |
blood,cells |
R3500 |
T5998 |
T5973 |
punct |
.,described |
R3501 |
T6000 |
T6001 |
det |
The,basis |
R3502 |
T6001 |
T6003 |
nsubj |
basis,are |
R3503 |
T6002 |
T6001 |
amod |
structural,basis |
R3504 |
T6004 |
T6001 |
prep |
for,basis |
R3505 |
T6005 |
T6006 |
det |
the,elasticy |
R3506 |
T6006 |
T6004 |
pobj |
elasticy,for |
R3507 |
T6007 |
T6006 |
prep |
of,elasticy |
R3508 |
T6008 |
T6009 |
amod |
red,cells |
R3509 |
T6009 |
T6007 |
pobj |
cells,of |
R3510 |
T6010 |
T6009 |
compound |
blood,cells |
R3511 |
T6011 |
T6009 |
prep |
in,cells |
R3512 |
T6012 |
T6011 |
pobj |
circulation,in |
R3513 |
T6013 |
T6014 |
amod |
long,range |
R3514 |
T6014 |
T6015 |
nmod |
range,functions |
R3515 |
T6015 |
T6003 |
attr |
functions,are |
R3516 |
T6016 |
T6015 |
amod |
molecular,functions |
R3517 |
T6017 |
T6015 |
prep |
of,functions |
R3518 |
T6018 |
T6019 |
det |
the,membrane |
R3519 |
T6019 |
T6017 |
pobj |
membrane,of |
R3520 |
T6020 |
T6019 |
compound |
plasma,membrane |
R3521 |
T6021 |
T6019 |
cc |
and,membrane |
R3522 |
T6022 |
T6023 |
det |
the,part |
R3523 |
T6023 |
T6019 |
conj |
part,membrane |
R3524 |
T6024 |
T6025 |
npadvmod |
membrane,associated |
R3525 |
T6025 |
T6023 |
amod |
associated,part |
R3526 |
T6026 |
T6023 |
prep |
of,part |
R3527 |
T6027 |
T6028 |
det |
the,cytoskeleton |
R3528 |
T6028 |
T6026 |
pobj |
cytoskeleton,of |
R3529 |
T6029 |
T6030 |
punct |
[,40 |
R3530 |
T6030 |
T6003 |
parataxis |
40,are |
R3531 |
T6031 |
T6032 |
punct |
-,43 |
R3532 |
T6032 |
T6030 |
prep |
43,40 |
R3533 |
T6033 |
T6030 |
punct |
],40 |
R3534 |
T6034 |
T6003 |
punct |
.,are |
R3535 |
T6036 |
T6037 |
amod |
Major,constituents |
R3536 |
T6037 |
T6038 |
nsubj |
constituents,are |
R3537 |
T6039 |
T6037 |
prep |
of,constituents |
R3538 |
T6040 |
T6041 |
det |
the,skeleton |
R3539 |
T6041 |
T6039 |
pobj |
skeleton,of |
R3540 |
T6042 |
T6041 |
compound |
membrane,skeleton |
R3541 |
T6043 |
T6044 |
compound |
spectrin,tetramers |
R3542 |
T6044 |
T6038 |
attr |
tetramers,are |
R3543 |
T6045 |
T6044 |
acl |
linked,tetramers |
R3544 |
T6046 |
T6045 |
advmod |
together,linked |
R3545 |
T6047 |
T6045 |
agent |
by,linked |
R3546 |
T6048 |
T6049 |
amod |
short,filaments |
R3547 |
T6049 |
T6047 |
pobj |
filaments,by |
R3548 |
T6050 |
T6049 |
compound |
actin,filaments |
R3549 |
T6051 |
T6049 |
cc |
and,filaments |
R3550 |
T6052 |
T6053 |
amod |
several,proteins |
R3551 |
T6053 |
T6049 |
conj |
proteins,filaments |
R3552 |
T6054 |
T6053 |
amod |
other,proteins |
R3553 |
T6055 |
T6053 |
acl |
covering,proteins |
R3554 |
T6056 |
T6057 |
det |
the,surface |
R3555 |
T6057 |
T6055 |
dobj |
surface,covering |
R3556 |
T6058 |
T6057 |
amod |
entire,surface |
R3557 |
T6059 |
T6057 |
amod |
cytoplasmic,surface |
R3558 |
T6060 |
T6057 |
prep |
of,surface |
R3559 |
T6061 |
T6062 |
det |
the,membrane |
R3560 |
T6062 |
T6060 |
pobj |
membrane,of |
R3561 |
T6063 |
T6064 |
amod |
red,cell |
R3562 |
T6064 |
T6062 |
compound |
cell,membrane |
R3563 |
T6065 |
T6064 |
compound |
blood,cell |
R3564 |
T6066 |
T6067 |
punct |
[,44 |
R3565 |
T6067 |
T6038 |
parataxis |
44,are |
R3566 |
T6068 |
T6067 |
punct |
],44 |
R3567 |
T6069 |
T6038 |
punct |
.,are |
R3568 |
T6071 |
T6072 |
compound |
Membrane,cholesterol |
R3569 |
T6072 |
T6073 |
nsubj |
cholesterol,diminishes |
R3570 |
T6074 |
T6075 |
amod |
red,cell |
R3571 |
T6075 |
T6077 |
compound |
cell,haemolysis |
R3572 |
T6076 |
T6075 |
compound |
blood,cell |
R3573 |
T6077 |
T6073 |
dobj |
haemolysis,diminishes |
R3574 |
T6078 |
T6077 |
prep |
by,haemolysis |
R3575 |
T6079 |
T6080 |
det |
the,complex |
R3576 |
T6080 |
T6078 |
pobj |
complex,by |
R3577 |
T6081 |
T6080 |
compound |
complement,complex |
R3578 |
T6082 |
T6073 |
punct |
", ",diminishes |
R3579 |
T6083 |
T6084 |
mark |
whereas,abrogates |
R3580 |
T6084 |
T6073 |
advcl |
abrogates,diminishes |
R3581 |
T6085 |
T6084 |
nsubj |
depletion,abrogates |
R3582 |
T6086 |
T6085 |
prep |
of,depletion |
R3583 |
T6087 |
T6088 |
compound |
raft,cholesterol |
R3584 |
T6088 |
T6086 |
pobj |
cholesterol,of |
R3585 |
T6089 |
T6084 |
dobj |
association,abrogates |
R3586 |
T6090 |
T6089 |
prep |
of,association |
R3587 |
T6091 |
T6092 |
det |
all,proteins |
R3588 |
T6092 |
T6090 |
pobj |
proteins,of |
R3589 |
T6093 |
T6092 |
compound |
raft,proteins |
R3590 |
T6094 |
T6084 |
prep |
with,abrogates |
R3591 |
T6095 |
T6096 |
det |
no,effect |
R3592 |
T6132 |
T6130 |
dobj |
resistance,have |
R3593 |
T6096 |
T6094 |
pobj |
effect,with |
R3594 |
T6097 |
T6096 |
amod |
significant,effect |
R3595 |
T6098 |
T6096 |
prep |
on,effect |
R3596 |
T6133 |
T6132 |
amod |
higher,resistance |
R3597 |
T6099 |
T6098 |
pobj |
areas,on |
R3598 |
T6134 |
T6132 |
amod |
osmotic,resistance |
R3599 |
T6135 |
T6124 |
punct |
.,show |
R3600 |
T6100 |
T6099 |
prep |
in,areas |
R3601 |
T6137 |
T6138 |
det |
The,presence |
R3602 |
T6101 |
T6102 |
det |
the,rest |
R3603 |
T6138 |
T6139 |
nsubj |
presence,alter |
R3604 |
T6140 |
T6138 |
prep |
of,presence |
R3605 |
T6102 |
T6100 |
pobj |
rest,in |
R3606 |
T6141 |
T6142 |
compound |
annexin,A7 |
R3607 |
T6142 |
T6140 |
pobj |
A7,of |
R3608 |
T6143 |
T6139 |
aux |
may,alter |
R3609 |
T6103 |
T6102 |
prep |
of,rest |
R3610 |
T6144 |
T6145 |
det |
the,flexibility |
R3611 |
T6145 |
T6139 |
dobj |
flexibility,alter |
R3612 |
T6104 |
T6105 |
det |
the,membrane |
R3613 |
T6146 |
T6145 |
compound |
membrane,flexibility |
R3614 |
T6147 |
T6148 |
mark |
in,supports |
R3615 |
T6148 |
T6139 |
advcl |
supports,alter |
R3616 |
T6105 |
T6103 |
pobj |
membrane,of |
R3617 |
T6149 |
T6148 |
mark |
that,supports |
R3618 |
T6150 |
T6148 |
nsubj |
it,supports |
R3619 |
T6106 |
T6105 |
compound |
cell,membrane |
R3620 |
T6151 |
T6152 |
det |
the,shape |
R3621 |
T6152 |
T6148 |
dobj |
shape,supports |
R3622 |
T6153 |
T6152 |
amod |
typical,shape |
R3623 |
T6107 |
T6099 |
cc |
and,areas |
R3624 |
T6154 |
T6152 |
compound |
biconcave,shape |
R3625 |
T6155 |
T6152 |
compound |
membrane,shape |
R3626 |
T6108 |
T6099 |
conj |
deformability,areas |
R3627 |
T6156 |
T6148 |
cc |
and,supports |
R3628 |
T6157 |
T6148 |
conj |
leads,supports |
R3629 |
T6158 |
T6157 |
prep |
to,leads |
R3630 |
T6159 |
T6160 |
det |
a,resistance |
R3631 |
T6109 |
T6110 |
punct |
[,46 |
R3632 |
T6160 |
T6158 |
pobj |
resistance,to |
R3633 |
T6161 |
T6160 |
amod |
lower,resistance |
R3634 |
T6162 |
T6160 |
amod |
minimal,resistance |
R3635 |
T6163 |
T6160 |
amod |
osmotic,resistance |
R3636 |
T6110 |
T6073 |
parataxis |
46,diminishes |
R3637 |
T6164 |
T6139 |
punct |
.,alter |
R3638 |
T6166 |
T6167 |
compound |
Annexin,A7 |
R3639 |
T6167 |
T6168 |
nsubj |
A7,appear |
R3640 |
T6111 |
T6110 |
nummod |
45,46 |
R3641 |
T6169 |
T6168 |
aux |
does,appear |
R3642 |
T6170 |
T6168 |
neg |
not,appear |
R3643 |
T6112 |
T6110 |
punct |
",",46 |
R3644 |
T6171 |
T6172 |
aux |
to,have |
R3645 |
T6172 |
T6168 |
xcomp |
have,appear |
R3646 |
T6173 |
T6174 |
det |
an,influence |
R3647 |
T6113 |
T6110 |
punct |
],46 |
R3648 |
T6174 |
T6172 |
dobj |
influence,have |
R3649 |
T6175 |
T6174 |
prep |
on,influence |
R3650 |
T6114 |
T6073 |
punct |
.,diminishes |
R3651 |
T6176 |
T6177 |
compound |
plasma,membrane |
R3652 |
T6177 |
T6178 |
compound |
membrane,integrity |
R3653 |
T6178 |
T6175 |
pobj |
integrity,on |
R3654 |
T6116 |
T6117 |
nmod |
Annexin,A7 |
R3655 |
T6179 |
T6178 |
appos |
itself,integrity |
R3656 |
T6180 |
T6181 |
mark |
as,stays |
R3657 |
T6181 |
T6172 |
advcl |
stays,have |
R3658 |
T6182 |
T6183 |
det |
the,value |
R3659 |
T6117 |
T6118 |
nmod |
A7,cells |
R3660 |
T6183 |
T6181 |
nsubj |
value,stays |
R3661 |
T6184 |
T6183 |
amod |
maximal,value |
R3662 |
T6185 |
T6186 |
amod |
osmotic,resistance |
R3663 |
T6118 |
T6124 |
nsubj |
cells,show |
R3664 |
T6186 |
T6183 |
compound |
resistance,value |
R3665 |
T6187 |
T6181 |
acomp |
constant,stays |
R3666 |
T6188 |
T6168 |
punct |
.,appear |
R3667 |
T6119 |
T6120 |
nmod |
knock,out |
R3668 |
T6190 |
T6191 |
nmod |
Ca2+,proteins |
R3669 |
T6120 |
T6121 |
nmod |
out,mouse |
R3670 |
T6191 |
T6195 |
nsubjpass |
proteins,known |
R3671 |
T6192 |
T6190 |
cc |
and,Ca2+ |
R3672 |
T6193 |
T6194 |
npadvmod |
membrane,skeletal |
R3673 |
T6121 |
T6118 |
nmod |
mouse,cells |
R3674 |
T6194 |
T6190 |
conj |
skeletal,Ca2+ |
R3675 |
T6122 |
T6118 |
amod |
red,cells |
R3676 |
T6196 |
T6195 |
auxpass |
are,known |
R3677 |
T6197 |
T6198 |
aux |
to,play |
R3678 |
T6198 |
T6195 |
xcomp |
play,known |
R3679 |
T6123 |
T6118 |
compound |
blood,cells |
R3680 |
T6199 |
T6200 |
amod |
key,roles |
R3681 |
T6200 |
T6198 |
dobj |
roles,play |
R3682 |
T6125 |
T6126 |
det |
a,shape |
R3683 |
T6201 |
T6198 |
prep |
on,play |
R3684 |
T6202 |
T6203 |
det |
the,shape |
R3685 |
T6203 |
T6201 |
pobj |
shape,on |
R3686 |
T6204 |
T6205 |
amod |
red,cell |
R3687 |
T6205 |
T6203 |
compound |
cell,shape |
R3688 |
T6206 |
T6205 |
compound |
blood,cell |
R3689 |
T6126 |
T6124 |
dobj |
shape,show |
R3690 |
T6127 |
T6128 |
advmod |
more,flat |
R3691 |
T6207 |
T6203 |
compound |
membrane,shape |
R3692 |
T6208 |
T6203 |
cc |
and,shape |
R3693 |
T6209 |
T6203 |
conj |
stability,shape |
R3694 |
T6128 |
T6126 |
amod |
flat,shape |
R3695 |
T6210 |
T6211 |
punct |
[,47 |
R3696 |
T6211 |
T6195 |
parataxis |
47,known |
R3697 |
T6129 |
T6124 |
cc |
and,show |
R3698 |
T6212 |
T6211 |
punct |
],47 |
R3699 |
T6213 |
T6195 |
punct |
.,known |
R3700 |
T6215 |
T6216 |
nsubjpass |
Annexins,reported |
R3701 |
T6130 |
T6124 |
conj |
have,show |
R3702 |
T6216 |
T6219 |
ccomp |
reported,exclude |
R3703 |
T6217 |
T6216 |
aux |
have,reported |
R3704 |
T6131 |
T6132 |
det |
a,resistance |
R3705 |
T6218 |
T6216 |
auxpass |
been,reported |
R3706 |
T6220 |
T6221 |
aux |
to,bind |
R3707 |
T6239 |
T6240 |
advmod |
Nevertheless,interact |
R3708 |
T6221 |
T6216 |
xcomp |
bind,reported |
R3709 |
T6222 |
T6221 |
advmod |
directly,bind |
R3710 |
T6223 |
T6221 |
prep |
to,bind |
R3711 |
T6224 |
T6225 |
compound |
F,actin |
R3712 |
T6225 |
T6223 |
pobj |
actin,to |
R3713 |
T6226 |
T6225 |
punct |
-,actin |
R3714 |
T6241 |
T6240 |
nsubj |
it,interact |
R3715 |
T6227 |
T6219 |
punct |
", ",exclude |
R3716 |
T6228 |
T6219 |
nsubj |
we,exclude |
R3717 |
T6229 |
T6219 |
aux |
could,exclude |
R3718 |
T6230 |
T6219 |
advmod |
however,exclude |
R3719 |
T6242 |
T6240 |
aux |
might,interact |
R3720 |
T6231 |
T6232 |
amod |
such,activity |
R3721 |
T6232 |
T6219 |
dobj |
activity,exclude |
R3722 |
T6243 |
T6240 |
prep |
with,interact |
R3723 |
T6233 |
T6232 |
det |
an,activity |
R3724 |
T6234 |
T6219 |
prep |
for,exclude |
R3725 |
T6244 |
T6245 |
amod |
other,components |
R3726 |
T6235 |
T6236 |
compound |
annexin,A7 |
R3727 |
T6236 |
T6234 |
pobj |
A7,for |
R3728 |
T6237 |
T6219 |
punct |
.,exclude |
R3729 |
T6245 |
T6243 |
pobj |
components,with |
R3730 |
T6246 |
T6240 |
cc |
and,interact |
R3731 |
T6247 |
T6240 |
conj |
have,interact |
R3732 |
T6248 |
T6249 |
det |
a,role |
R3733 |
T6249 |
T6247 |
dobj |
role,have |
R3734 |
T6344 |
T6345 |
amod |
cytoskeletal,protein |
R3735 |
T6250 |
T6247 |
prep |
in,have |
R3736 |
T6345 |
T6346 |
compound |
protein,interactions |
R3737 |
T6346 |
T6343 |
pobj |
interactions,of |
R3738 |
T6347 |
T6348 |
punct |
[,50 |
R3739 |
T6348 |
T6330 |
parataxis |
50,shown |
R3740 |
T6349 |
T6348 |
punct |
],50 |
R3741 |
T6350 |
T6330 |
punct |
.,shown |
R3742 |
T6251 |
T6250 |
pcomp |
organizing,in |
R3743 |
T6352 |
T6353 |
nsubj |
Ca2+,induces |
R3744 |
T6252 |
T6253 |
det |
the,cytoskeleton |
R3745 |
T6354 |
T6353 |
advmod |
also,induces |
R3746 |
T6355 |
T6356 |
det |
a,redistribution |
R3747 |
T6356 |
T6353 |
dobj |
redistribution,induces |
R3748 |
T6253 |
T6251 |
dobj |
cytoskeleton,organizing |
R3749 |
T6357 |
T6356 |
compound |
transbilayer,redistribution |
R3750 |
T6358 |
T6356 |
compound |
phospholipid,redistribution |
R3751 |
T6254 |
T6253 |
compound |
membrane,cytoskeleton |
R3752 |
T6359 |
T6356 |
acl |
inducing,redistribution |
R3753 |
T6360 |
T6361 |
det |
a,change |
R3754 |
T6361 |
T6359 |
dobj |
change,inducing |
R3755 |
T6255 |
T6240 |
punct |
", ",interact |
R3756 |
T6362 |
T6361 |
compound |
shape,change |
R3757 |
T6363 |
T6361 |
prep |
of,change |
R3758 |
T6256 |
T6240 |
advcl |
controlling,interact |
R3759 |
T6364 |
T6365 |
amod |
red,cells |
R3760 |
T6365 |
T6363 |
pobj |
cells,of |
R3761 |
T6366 |
T6365 |
compound |
blood,cells |
R3762 |
T6257 |
T6258 |
compound |
raft,protein |
R3763 |
T6367 |
T6368 |
punct |
[,51 |
R3764 |
T6368 |
T6353 |
parataxis |
51,induces |
R3765 |
T6369 |
T6368 |
punct |
],51 |
R3766 |
T6258 |
T6259 |
compound |
protein,associations |
R3767 |
T6370 |
T6353 |
punct |
.,induces |
R3768 |
T6372 |
T6373 |
prep |
In,drive |
R3769 |
T6259 |
T6256 |
dobj |
associations,controlling |
R3770 |
T6374 |
T6372 |
pobj |
addition,In |
R3771 |
T6260 |
T6256 |
cc |
or,controlling |
R3772 |
T6375 |
T6373 |
punct |
", ",drive |
R3773 |
T6376 |
T6377 |
compound |
Ca2+,ions |
R3774 |
T6377 |
T6373 |
nsubj |
ions,drive |
R3775 |
T6261 |
T6256 |
conj |
influencing,controlling |
R3776 |
T6378 |
T6373 |
aux |
can,drive |
R3777 |
T6379 |
T6373 |
dobj |
spiculation,drive |
R3778 |
T6380 |
T6379 |
prep |
of,spiculation |
R3779 |
T6381 |
T6382 |
det |
the,bilayer |
R3780 |
T6262 |
T6263 |
det |
the,strength |
R3781 |
T6382 |
T6380 |
pobj |
bilayer,of |
R3782 |
T6383 |
T6382 |
compound |
membrane,bilayer |
R3783 |
T6263 |
T6261 |
dobj |
strength,influencing |
R3784 |
T6384 |
T6373 |
prep |
without,drive |
R3785 |
T6385 |
T6384 |
pcomp |
involving,without |
R3786 |
T6386 |
T6387 |
det |
the,cytoskeleton |
R3787 |
T6264 |
T6263 |
amod |
ionic,strength |
R3788 |
T6387 |
T6385 |
dobj |
cytoskeleton,involving |
R3789 |
T6388 |
T6389 |
punct |
[,52 |
R3790 |
T6389 |
T6373 |
parataxis |
52,drive |
R3791 |
T6390 |
T6389 |
punct |
],52 |
R3792 |
T6391 |
T6373 |
punct |
.,drive |
R3793 |
T6265 |
T6263 |
prep |
of,strength |
R3794 |
T6393 |
T6394 |
nsubj |
We,observed |
R3795 |
T6266 |
T6267 |
amod |
red,cells |
R3796 |
T6395 |
T6394 |
aux |
have,observed |
R3797 |
T6396 |
T6394 |
advmod |
also,observed |
R3798 |
T6397 |
T6398 |
det |
a,defect |
R3799 |
T6267 |
T6265 |
pobj |
cells,of |
R3800 |
T6268 |
T6267 |
compound |
blood,cells |
R3801 |
T6398 |
T6394 |
dobj |
defect,observed |
R3802 |
T6269 |
T6256 |
prep |
on,controlling |
R3803 |
T6399 |
T6398 |
prep |
in,defect |
R3804 |
T6270 |
T6271 |
poss |
its,own |
R3805 |
T6400 |
T6401 |
compound |
platelet,function |
R3806 |
T6401 |
T6399 |
pobj |
function,in |
R3807 |
T6402 |
T6394 |
prep |
in,observed |
R3808 |
T6403 |
T6404 |
det |
the,mutant |
R3809 |
T6271 |
T6269 |
pobj |
own,on |
R3810 |
T6404 |
T6402 |
pobj |
mutant,in |
R3811 |
T6405 |
T6406 |
compound |
annexin,A7 |
R3812 |
T6406 |
T6404 |
compound |
A7,mutant |
R3813 |
T6272 |
T6269 |
cc |
or,on |
R3814 |
T6407 |
T6394 |
punct |
.,observed |
R3815 |
T6273 |
T6269 |
conj |
by,on |
R3816 |
T6409 |
T6410 |
prep |
In,was |
R3817 |
T6411 |
T6412 |
det |
the,experiments |
R3818 |
T6274 |
T6273 |
pcomp |
interfering,by |
R3819 |
T6412 |
T6409 |
pobj |
experiments,In |
R3820 |
T6413 |
T6414 |
compound |
platelet,aggregation |
R3821 |
T6414 |
T6412 |
compound |
aggregation,experiments |
R3822 |
T6275 |
T6274 |
prep |
with,interfering |
R3823 |
T6415 |
T6416 |
det |
the,velocity |
R3824 |
T6416 |
T6410 |
nsubj |
velocity,was |
R3825 |
T6417 |
T6416 |
amod |
initial,velocity |
R3826 |
T6276 |
T6277 |
amod |
other,pathways |
R3827 |
T6418 |
T6416 |
compound |
aggregation,velocity |
R3828 |
T6419 |
T6420 |
advmod |
slightly,lower |
R3829 |
T6277 |
T6275 |
pobj |
pathways,with |
R3830 |
T6420 |
T6410 |
acomp |
lower,was |
R3831 |
T6421 |
T6419 |
punct |
", ",slightly |
R3832 |
T6422 |
T6419 |
cc |
but,slightly |
R3833 |
T6423 |
T6419 |
conj |
significantly,slightly |
R3834 |
T6278 |
T6277 |
compound |
signaling,pathways |
R3835 |
T6424 |
T6410 |
prep |
in,was |
R3836 |
T6425 |
T6424 |
pobj |
platelets,in |
R3837 |
T6279 |
T6240 |
punct |
.,interact |
R3838 |
T6426 |
T6425 |
acl |
lacking,platelets |
R3839 |
T6427 |
T6428 |
compound |
annexin,A7 |
R3840 |
T6428 |
T6426 |
dobj |
A7,lacking |
R3841 |
T6281 |
T6282 |
mark |
As,discussed |
R3842 |
T6429 |
T6410 |
punct |
.,was |
R3843 |
T6431 |
T6432 |
det |
These,data |
R3844 |
T6432 |
T6433 |
nsubjpass |
data,reflected |
R3845 |
T6434 |
T6433 |
auxpass |
are,reflected |
R3846 |
T6435 |
T6433 |
neg |
not,reflected |
R3847 |
T6282 |
T6283 |
advcl |
discussed,involved |
R3848 |
T6436 |
T6433 |
agent |
by,reflected |
R3849 |
T6437 |
T6438 |
det |
a,change |
R3850 |
T6438 |
T6436 |
pobj |
change,by |
R3851 |
T6284 |
T6282 |
advmod |
above,discussed |
R3852 |
T6439 |
T6438 |
prep |
in,change |
R3853 |
T6440 |
T6441 |
det |
the,time |
R3854 |
T6441 |
T6439 |
pobj |
time,in |
R3855 |
T6442 |
T6443 |
advmod |
in,vivo |
R3856 |
T6285 |
T6286 |
compound |
annexin,A7 |
R3857 |
T6443 |
T6441 |
amod |
vivo,time |
R3858 |
T6444 |
T6441 |
compound |
bleeding,time |
R3859 |
T6445 |
T6433 |
punct |
.,reflected |
R3860 |
T6286 |
T6283 |
nsubjpass |
A7,involved |
R3861 |
T6447 |
T6448 |
advmod |
However,is |
R3862 |
T6449 |
T6450 |
det |
the,speed |
R3863 |
T6287 |
T6283 |
aux |
could,involved |
R3864 |
T6288 |
T6283 |
auxpass |
be,involved |
R3865 |
T6289 |
T6283 |
prep |
in,involved |
R3866 |
T6290 |
T6291 |
det |
the,homeostasis |
R3867 |
T6291 |
T6289 |
pobj |
homeostasis,in |
R3868 |
T6292 |
T6293 |
amod |
cytoplasmic,Ca2+ |
R3869 |
T6450 |
T6448 |
nsubj |
speed,is |
R3870 |
T6293 |
T6291 |
compound |
Ca2+,homeostasis |
R3871 |
T6451 |
T6450 |
compound |
reaction,speed |
R3872 |
T6452 |
T6450 |
prep |
of,speed |
R3873 |
T6294 |
T6283 |
punct |
.,involved |
R3874 |
T6453 |
T6454 |
amod |
murine,platelets |
R3875 |
T6454 |
T6452 |
pobj |
platelets,of |
R3876 |
T6296 |
T6297 |
nsubjpass |
It,demonstrated |
R3877 |
T6455 |
T6456 |
advmod |
much,higher |
R3878 |
T6456 |
T6448 |
acomp |
higher,is |
R3879 |
T6457 |
T6456 |
prep |
than,higher |
R3880 |
T6458 |
T6457 |
pobj |
those,than |
R3881 |
T6298 |
T6297 |
aux |
has,demonstrated |
R3882 |
T6459 |
T6458 |
prep |
of,those |
R3883 |
T6460 |
T6459 |
pobj |
human,of |
R3884 |
T6461 |
T6448 |
cc |
and,is |
R3885 |
T6462 |
T6463 |
prep |
due,missed |
R3886 |
T6299 |
T6297 |
auxpass |
been,demonstrated |
R3887 |
T6463 |
T6448 |
conj |
missed,is |
R3888 |
T6464 |
T6462 |
pcomp |
to,due |
R3889 |
T6300 |
T6301 |
mark |
that,exert |
R3890 |
T6465 |
T6466 |
det |
the,setup |
R3891 |
T6466 |
T6462 |
pobj |
setup,due |
R3892 |
T6467 |
T6466 |
amod |
technical,setup |
R3893 |
T6301 |
T6297 |
ccomp |
exert,demonstrated |
R3894 |
T6468 |
T6463 |
nsubj |
we,missed |
R3895 |
T6469 |
T6463 |
aux |
might,missed |
R3896 |
T6470 |
T6463 |
aux |
have,missed |
R3897 |
T6471 |
T6472 |
amod |
larger,changes |
R3898 |
T6472 |
T6463 |
dobj |
changes,missed |
R3899 |
T6473 |
T6463 |
prep |
in,missed |
R3900 |
T6302 |
T6303 |
amod |
micromolar,changes |
R3901 |
T6474 |
T6475 |
det |
the,samples |
R3902 |
T6475 |
T6473 |
pobj |
samples,in |
R3903 |
T6476 |
T6475 |
compound |
mouse,samples |
R3904 |
T6303 |
T6301 |
nsubj |
changes,exert |
R3905 |
T6477 |
T6463 |
punct |
.,missed |
R3906 |
T6304 |
T6303 |
prep |
of,changes |
R3907 |
T6479 |
T6480 |
det |
The,reaction |
R3908 |
T6480 |
T6482 |
nsubjpass |
reaction,caused |
R3909 |
T6481 |
T6480 |
amod |
immediate,reaction |
R3910 |
T6305 |
T6306 |
det |
the,concentration |
R3911 |
T6483 |
T6480 |
prep |
of,reaction |
R3912 |
T6306 |
T6304 |
pobj |
concentration,of |
R3913 |
T6484 |
T6485 |
compound |
mouse,platelets |
R3914 |
T6485 |
T6483 |
pobj |
platelets,of |
R3915 |
T6307 |
T6306 |
amod |
intracellular,concentration |
R3916 |
T6486 |
T6480 |
cc |
and,reaction |
R3917 |
T6308 |
T6306 |
compound |
Ca2+,concentration |
R3919 |
T6309 |
T6310 |
det |
a,effect |
R3920 |
T6488 |
T6480 |
conj |
absence,reaction |
R3921 |
T6489 |
T6488 |
prep |
of,absence |
R3922 |
T6310 |
T6301 |
dobj |
effect,exert |
R3923 |
T6490 |
T6491 |
det |
a,phase |
R3924 |
T6491 |
T6489 |
pobj |
phase,of |
R3925 |
T6492 |
T6491 |
compound |
lag,phase |
R3926 |
T6493 |
T6491 |
prep |
after,phase |
R3927 |
T6311 |
T6310 |
amod |
profound,effect |
R3928 |
T6494 |
T6493 |
pobj |
triggering,after |
R3929 |
T6495 |
T6482 |
aux |
may,caused |
R3930 |
T6312 |
T6310 |
prep |
on,effect |
R3931 |
T6496 |
T6482 |
auxpass |
be,caused |
R3932 |
T6497 |
T6482 |
agent |
by,caused |
R3933 |
T6313 |
T6314 |
det |
the,properties |
R3934 |
T6498 |
T6499 |
det |
an,generation |
R3935 |
T6499 |
T6497 |
pobj |
generation,by |
R3936 |
T6500 |
T6501 |
advmod |
immediately,initiated |
R3937 |
T6314 |
T6312 |
pobj |
properties,on |
R3938 |
T6501 |
T6499 |
amod |
initiated,generation |
R3939 |
T6502 |
T6499 |
compound |
thrombin,generation |
R3940 |
T6315 |
T6314 |
compound |
membrane,properties |
R3941 |
T6503 |
T6504 |
punct |
[,22 |
R3942 |
T6504 |
T6482 |
parataxis |
22,caused |
R3943 |
T6505 |
T6504 |
punct |
],22 |
R3944 |
T6316 |
T6317 |
dep |
that,regulate |
R3945 |
T6506 |
T6482 |
punct |
.,caused |
R3946 |
T6508 |
T6509 |
mark |
As,known |
R3947 |
T6509 |
T6510 |
advcl |
known,are |
R3948 |
T6511 |
T6509 |
prep |
from,known |
R3949 |
T6317 |
T6314 |
relcl |
regulate,properties |
R3950 |
T6512 |
T6513 |
det |
the,syndrome |
R3951 |
T6513 |
T6511 |
pobj |
syndrome,from |
R3952 |
T6514 |
T6513 |
amod |
frequent,syndrome |
R3953 |
T6318 |
T6319 |
amod |
red,cell |
R3954 |
T6515 |
T6513 |
amod |
human,syndrome |
R3955 |
T6516 |
T6513 |
compound |
vWF,syndrome |
R3956 |
T6517 |
T6513 |
punct |
-,syndrome |
R3957 |
T6319 |
T6321 |
compound |
cell,deformability |
R3958 |
T6518 |
T6513 |
cc |
or,syndrome |
R3959 |
T6519 |
T6520 |
amod |
other,diseases |
R3960 |
T6320 |
T6319 |
compound |
blood,cell |
R3961 |
T6520 |
T6513 |
conj |
diseases,syndrome |
R3962 |
T6521 |
T6520 |
amod |
haemostatic,diseases |
R3963 |
T6522 |
T6510 |
punct |
", ",are |
R3964 |
T6321 |
T6317 |
dobj |
deformability,regulate |
R3965 |
T6523 |
T6524 |
amod |
small,defects |
R3966 |
T6524 |
T6510 |
nsubj |
defects,are |
R3967 |
T6322 |
T6323 |
punct |
[,49 |
R3968 |
T6525 |
T6510 |
advmod |
often,are |
R3969 |
T6526 |
T6527 |
advmod |
clinically,silent |
R3970 |
T6527 |
T6510 |
acomp |
silent,are |
R3971 |
T6323 |
T6297 |
parataxis |
49,demonstrated |
R3972 |
T6528 |
T6510 |
cc |
and,are |
R3973 |
T6529 |
T6530 |
aux |
do,result |
R3974 |
T6324 |
T6323 |
nummod |
48,49 |
R3975 |
T6530 |
T6510 |
conj |
result,are |
R3976 |
T6531 |
T6530 |
neg |
not,result |
R3977 |
T6532 |
T6530 |
prep |
in,result |
R3978 |
T6533 |
T6534 |
amod |
physiological,changes |
R3979 |
T6325 |
T6323 |
punct |
",",49 |
R3980 |
T6534 |
T6532 |
pobj |
changes,in |
R3981 |
T6535 |
T6510 |
prep |
under,are |
R3982 |
T6326 |
T6323 |
punct |
],49 |
R3983 |
T6536 |
T6537 |
amod |
normal,conditions |
R3984 |
T6537 |
T6535 |
pobj |
conditions,under |
R3985 |
T6538 |
T6510 |
punct |
.,are |
R3986 |
T6327 |
T6297 |
punct |
.,demonstrated |
R3987 |
T6540 |
T6541 |
advmod |
Only,in |
R3988 |
T6541 |
T6542 |
prep |
in,become |
R3989 |
T6329 |
T6330 |
advmod |
Furthermore,shown |
R3990 |
T6543 |
T6541 |
pobj |
situations,in |
R3991 |
T6544 |
T6543 |
prep |
with,situations |
R3992 |
T6545 |
T6546 |
amod |
severe,injury |
R3993 |
T6331 |
T6330 |
punct |
", ",shown |
R3994 |
T6546 |
T6544 |
pobj |
injury,with |
R3995 |
T6547 |
T6546 |
amod |
physical,injury |
R3996 |
T6548 |
T6542 |
punct |
", ",become |
R3997 |
T6549 |
T6550 |
amod |
distinct,influences |
R3998 |
T6550 |
T6542 |
nsubj |
influences,become |
R3999 |
T6551 |
T6550 |
amod |
environmental,influences |
R4000 |
T6552 |
T6550 |
cc |
or,influences |
R4001 |
T6332 |
T6333 |
det |
the,Ca2+ |
R4002 |
T6553 |
T6554 |
amod |
additional,platelet |
R4003 |
T6554 |
T6550 |
conj |
platelet,influences |
R4004 |
T6555 |
T6554 |
nmod |
attributes,platelet |
R4005 |
T6333 |
T6330 |
nsubjpass |
Ca2+,shown |
R4006 |
T6334 |
T6333 |
amod |
intracellular,Ca2+ |
R4007 |
T6335 |
T6330 |
auxpass |
was,shown |
R4008 |
T6556 |
T6554 |
prep |
like,platelet |
R4009 |
T6557 |
T6558 |
det |
the,differences |
R4010 |
T6336 |
T6337 |
aux |
to,regulate |
R4011 |
T6558 |
T6556 |
pobj |
differences,like |
R4012 |
T6559 |
T6560 |
nmod |
platelet,antigen |
R4013 |
T6337 |
T6330 |
xcomp |
regulate,shown |
R4014 |
T6560 |
T6558 |
nmod |
antigen,differences |
R4015 |
T6561 |
T6558 |
nmod |
polymorphism,differences |
R4016 |
T6562 |
T6563 |
punct |
[,53 |
R4017 |
T6338 |
T6339 |
det |
the,stability |
R4018 |
T6563 |
T6558 |
parataxis |
53,differences |
R4019 |
T6564 |
T6563 |
punct |
],53 |
R4020 |
T6565 |
T6542 |
aux |
might,become |
R4021 |
T6339 |
T6337 |
dobj |
stability,regulate |
R4022 |
T6566 |
T6542 |
acomp |
apparent,become |
R4023 |
T6567 |
T6542 |
punct |
.,become |
R4024 |
T6340 |
T6339 |
compound |
membrane,stability |
R4025 |
T6341 |
T6337 |
prep |
through,regulate |
R4026 |
T6342 |
T6341 |
pobj |
modulation,through |
R4027 |
T6343 |
T6342 |
prep |
of,modulation |
R4028 |
T6643 |
T6644 |
prep |
In,report |
R4029 |
T6645 |
T6646 |
det |
this,paper |
R4030 |
T6646 |
T6643 |
pobj |
paper,In |
R4031 |
T6647 |
T6644 |
nsubj |
we,report |
R4032 |
T6648 |
T6649 |
det |
the,presence |
R4033 |
T6649 |
T6644 |
dobj |
presence,report |
R4034 |
T6650 |
T6649 |
prep |
of,presence |
R4035 |
T6651 |
T6652 |
preconj |
both,isoforms |
R4036 |
T6652 |
T6650 |
pobj |
isoforms,of |
R4037 |
T6653 |
T6654 |
compound |
annexin,A7 |
R4038 |
T6654 |
T6652 |
compound |
A7,isoforms |
R4039 |
T6655 |
T6649 |
prep |
in,presence |
R4040 |
T6656 |
T6657 |
amod |
red,cells |
R4041 |
T6657 |
T6655 |
pobj |
cells,in |
R4042 |
T6658 |
T6657 |
compound |
blood,cells |
R4043 |
T6659 |
T6660 |
advmod |
where,are |
R4044 |
T6660 |
T6657 |
relcl |
are,cells |
R4045 |
T6661 |
T6660 |
nsubj |
they,are |
R4046 |
T6662 |
T6660 |
acomp |
abundant,are |
R4047 |
T6663 |
T6660 |
prep |
in,are |
R4048 |
T6664 |
T6663 |
pobj |
nanovesicles,in |
R4049 |
T6665 |
T6666 |
dep |
that,form |
R4050 |
T6666 |
T6664 |
relcl |
form,nanovesicles |
R4051 |
T6667 |
T6666 |
prep |
upon,form |
R4052 |
T6668 |
T6669 |
compound |
Ca2+,addition |
R4053 |
T6669 |
T6667 |
pobj |
addition,upon |
R4054 |
T6670 |
T6644 |
punct |
.,report |
R4055 |
T6672 |
T6673 |
det |
The,proteins |
R4056 |
T6673 |
T6674 |
nsubjpass |
proteins,required |
R4057 |
T6675 |
T6674 |
auxpass |
are,required |
R4058 |
T6676 |
T6674 |
advmod |
however,required |
R4059 |
T6677 |
T6674 |
neg |
not,required |
R4060 |
T6678 |
T6674 |
prep |
for,required |
R4061 |
T6679 |
T6680 |
det |
the,process |
R4062 |
T6680 |
T6678 |
pobj |
process,for |
R4063 |
T6681 |
T6680 |
compound |
vesiculation,process |
R4064 |
T6682 |
T6674 |
punct |
.,required |
R4065 |
T6684 |
T6685 |
nsubj |
They,are |
R4066 |
T6686 |
T6685 |
advmod |
also,are |
R4067 |
T6687 |
T6685 |
neg |
not,are |
R4068 |
T6688 |
T6685 |
acomp |
essential,are |
R4069 |
T6689 |
T6688 |
prep |
for,essential |
R4070 |
T6690 |
T6691 |
det |
the,formation |
R4071 |
T6691 |
T6689 |
pobj |
formation,for |
R4072 |
T6692 |
T6691 |
prep |
of,formation |
R4073 |
T6693 |
T6694 |
amod |
specific,domains |
R4074 |
T6694 |
T6692 |
pobj |
domains,of |
R4075 |
T6695 |
T6694 |
compound |
membrane,domains |
R4076 |
T6696 |
T6697 |
amod |
such,as |
R4077 |
T6697 |
T6694 |
prep |
as,domains |
R4078 |
T6698 |
T6699 |
compound |
lipid,rafts |
R4079 |
T6699 |
T6697 |
pobj |
rafts,as |
R4080 |
T6700 |
T6685 |
punct |
", ",are |
R4081 |
T6701 |
T6685 |
prep |
as,are |
R4082 |
T6702 |
T6703 |
advmod |
exogenously,added |
R4083 |
T6703 |
T6704 |
amod |
added,A7 |
R4084 |
T6704 |
T6701 |
pobj |
A7,as |
R4085 |
T6705 |
T6704 |
amod |
recombinant,A7 |
R4086 |
T6706 |
T6704 |
compound |
annexin,A7 |
R4087 |
T6707 |
T6704 |
acl |
redistributed,A7 |
R4088 |
T6708 |
T6707 |
prep |
to,redistributed |
R4089 |
T6709 |
T6710 |
amod |
similar,positions |
R4090 |
T6710 |
T6708 |
pobj |
positions,to |
R4091 |
T6711 |
T6704 |
prep |
in,A7 |
R4092 |
T6712 |
T6713 |
det |
a,gradient |
R4093 |
T6713 |
T6711 |
pobj |
gradient,in |
R4094 |
T6714 |
T6713 |
compound |
density,gradient |
R4095 |
T6715 |
T6713 |
acl |
containing,gradient |
R4096 |
T6716 |
T6715 |
dobj |
membranes,containing |
R4097 |
T6717 |
T6716 |
acl |
derived,membranes |
R4098 |
T6718 |
T6717 |
prep |
from,derived |
R4099 |
T6719 |
T6720 |
amod |
red,cells |
R4100 |
T6720 |
T6718 |
pobj |
cells,from |
R4101 |
T6721 |
T6720 |
compound |
blood,cells |
R4102 |
T6722 |
T6720 |
prep |
of,cells |
R4103 |
T6723 |
T6724 |
nmod |
anxA7,mice |
R4104 |
T6724 |
T6722 |
pobj |
mice,of |
R4105 |
T6725 |
T6723 |
punct |
-,anxA7 |
R4106 |
T6726 |
T6723 |
punct |
/,anxA7 |
R4107 |
T6727 |
T6723 |
punct |
-,anxA7 |
R4108 |
T6728 |
T6685 |
punct |
.,are |
R4109 |
T6730 |
T6731 |
det |
The,change |
R4110 |
T6731 |
T6735 |
nsubj |
change,be |
R4111 |
T6732 |
T6731 |
amod |
observed,change |
R4112 |
T6733 |
T6734 |
compound |
cell,shape |
R4113 |
T6734 |
T6731 |
compound |
shape,change |
R4114 |
T6736 |
T6731 |
prep |
of,change |
R4115 |
T6737 |
T6736 |
pobj |
erythrocytes,of |
R4116 |
T6738 |
T6737 |
acl |
lacking,erythrocytes |
R4117 |
T6739 |
T6740 |
compound |
annexin,A7 |
R4118 |
T6740 |
T6738 |
dobj |
A7,lacking |
R4119 |
T6741 |
T6735 |
aux |
might,be |
R4120 |
T6742 |
T6735 |
prep |
due,be |
R4121 |
T6743 |
T6742 |
pcomp |
to,due |
R4122 |
T6744 |
T6742 |
pobj |
alterations,due |
R4123 |
T6745 |
T6744 |
prep |
in,alterations |
R4124 |
T6746 |
T6747 |
det |
the,cytoskeleton |
R4125 |
T6747 |
T6745 |
pobj |
cytoskeleton,in |
R4126 |
T6748 |
T6747 |
compound |
erythrocyte,cytoskeleton |
R4127 |
T6749 |
T6735 |
punct |
.,be |
R4128 |
T6751 |
T6752 |
mark |
As,ruled |
R4129 |
T6752 |
T6761 |
advcl |
ruled,mediated |
R4130 |
T6753 |
T6754 |
det |
a,interaction |
R4131 |
T6754 |
T6752 |
nsubjpass |
interaction,ruled |
R4132 |
T6755 |
T6754 |
amod |
direct,interaction |
R4133 |
T6756 |
T6754 |
prep |
with,interaction |
R4134 |
T6757 |
T6758 |
compound |
F,actin |
R4135 |
T6758 |
T6756 |
pobj |
actin,with |
R4136 |
T6759 |
T6758 |
punct |
-,actin |
R4137 |
T6760 |
T6752 |
auxpass |
was,ruled |
R4138 |
T6762 |
T6752 |
prt |
out,ruled |
R4139 |
T6763 |
T6761 |
punct |
", ",mediated |
R4140 |
T6764 |
T6765 |
det |
this,effect |
R4141 |
T6765 |
T6761 |
nsubjpass |
effect,mediated |
R4142 |
T6766 |
T6761 |
aux |
might,mediated |
R4143 |
T6767 |
T6761 |
auxpass |
be,mediated |
R4144 |
T6768 |
T6761 |
agent |
by,mediated |
R4145 |
T6769 |
T6770 |
poss |
annexin,activity |
R4146 |
T6770 |
T6768 |
pobj |
activity,by |
R4147 |
T6771 |
T6769 |
case |
's,annexin |
R4148 |
T6772 |
T6773 |
npadvmod |
Ca2+,binding |
R4149 |
T6773 |
T6770 |
amod |
binding,activity |
R4150 |
T6774 |
T6773 |
punct |
-,binding |
R4151 |
T6775 |
T6761 |
punct |
.,mediated |
R4152 |
T6880 |
T6879 |
prep |
of,Acquisition |
R4153 |
T6881 |
T6882 |
nmod |
murine,blood |
R4154 |
T6882 |
T6885 |
compound |
blood,samples |
R4155 |
T6883 |
T6881 |
cc |
and,murine |
R4156 |
T6884 |
T6881 |
conj |
human,murine |
R4157 |
T6885 |
T6880 |
pobj |
samples,of |
R4158 |
T6887 |
T6888 |
nsubjpass |
Blood,collected |
R4159 |
T6889 |
T6887 |
prep |
from,Blood |
R4160 |
T6890 |
T6891 |
nmod |
129Sv,mice |
R4161 |
T6891 |
T6889 |
pobj |
mice,from |
R4162 |
T6892 |
T6893 |
amod |
wild,type |
R4163 |
T6893 |
T6891 |
nmod |
type,mice |
R4164 |
T6894 |
T6893 |
cc |
and,type |
R4165 |
T6895 |
T6896 |
compound |
annexin,A7 |
R4166 |
T6896 |
T6893 |
conj |
A7,type |
R4167 |
T6897 |
T6898 |
compound |
knock,out |
R4168 |
T6898 |
T6891 |
compound |
out,mice |
R4169 |
T6899 |
T6900 |
punct |
(,anxA7 |
R4170 |
T6900 |
T6891 |
parataxis |
anxA7,mice |
R4171 |
T6901 |
T6900 |
punct |
-,anxA7 |
R4172 |
T6902 |
T6900 |
punct |
/,anxA7 |
R4173 |
T6903 |
T6900 |
punct |
-,anxA7 |
R4174 |
T6904 |
T6900 |
punct |
),anxA7 |
R4175 |
T6905 |
T6887 |
acl |
kept,Blood |
R4176 |
T6906 |
T6905 |
prep |
under,kept |
R4177 |
T6907 |
T6908 |
npadvmod |
pathogen,free |
R4178 |
T6908 |
T6910 |
amod |
free,conditions |
R4179 |
T6909 |
T6908 |
punct |
-,free |
R4180 |
T6910 |
T6906 |
pobj |
conditions,under |
R4181 |
T6911 |
T6905 |
cc |
and,kept |
R4182 |
T6912 |
T6905 |
conj |
fed,kept |
R4183 |
T6913 |
T6912 |
prep |
with,fed |
R4184 |
T6914 |
T6915 |
amod |
regular,food |
R4185 |
T6915 |
T6913 |
pobj |
food,with |
R4186 |
T6916 |
T6888 |
punct |
", ",collected |
R4187 |
T6917 |
T6888 |
auxpass |
was,collected |
R4188 |
T6918 |
T6888 |
prep |
in,collected |
R4189 |
T6919 |
T6920 |
nummod |
1,ml |
R4190 |
T6920 |
T6921 |
compound |
ml,syringes |
R4191 |
T6921 |
T6918 |
pobj |
syringes,in |
R4192 |
T6922 |
T6888 |
prep |
with,collected |
R4193 |
T6923 |
T6924 |
det |
a,needle |
R4194 |
T6924 |
T6927 |
nsubj |
needle,punctating |
R4195 |
T6925 |
T6926 |
nummod |
22,gauge |
R4196 |
T6926 |
T6924 |
compound |
gauge,needle |
R4197 |
T6927 |
T6922 |
pcomp |
punctating,with |
R4198 |
T6928 |
T6929 |
det |
the,ventricle |
R4199 |
T6929 |
T6927 |
dobj |
ventricle,punctating |
R4200 |
T6930 |
T6929 |
amod |
right,ventricle |
R4201 |
T6931 |
T6929 |
amod |
cardiac,ventricle |
R4202 |
T6932 |
T6933 |
advmod |
immediately,killed |
R4203 |
T6933 |
T6927 |
advcl |
killed,punctating |
R4204 |
T6934 |
T6933 |
mark |
after,killed |
R4205 |
T6935 |
T6933 |
nsubjpass |
they,killed |
R4206 |
T6936 |
T6933 |
aux |
had,killed |
R4207 |
T6937 |
T6933 |
auxpass |
been,killed |
R4208 |
T6938 |
T6933 |
prep |
by,killed |
R4209 |
T6939 |
T6940 |
amod |
cervical,dislocation |
R4210 |
T6940 |
T6938 |
pobj |
dislocation,by |
R4211 |
T6941 |
T6888 |
punct |
.,collected |
R4212 |
T6943 |
T6944 |
nsubjpass |
Coagulation,inhibited |
R4213 |
T6945 |
T6944 |
advmod |
generally,inhibited |
R4214 |
T6946 |
T6944 |
auxpass |
was,inhibited |
R4215 |
T6947 |
T6944 |
prep |
with,inhibited |
R4216 |
T6948 |
T6949 |
quantmod |
1,10 |
R4217 |
T6949 |
T6951 |
nummod |
10,volume |
R4218 |
T6950 |
T6949 |
punct |
/,10 |
R4219 |
T6951 |
T6947 |
pobj |
volume,with |
R4220 |
T6952 |
T6951 |
prep |
of,volume |
R4221 |
T6953 |
T6954 |
nummod |
10,mM |
R4222 |
T6954 |
T6955 |
compound |
mM,NaEDTA |
R4223 |
T6955 |
T6952 |
pobj |
NaEDTA,of |
R4224 |
T6956 |
T6955 |
punct |
", ",NaEDTA |
R4225 |
T6957 |
T6955 |
npadvmod |
pH,NaEDTA |
R4226 |
T6958 |
T6957 |
nummod |
7.5,pH |
R4227 |
T6959 |
T6951 |
punct |
", ",volume |
R4228 |
T6960 |
T6961 |
advmod |
already,present |
R4229 |
T6961 |
T6951 |
relcl |
present,volume |
R4230 |
T6962 |
T6961 |
prep |
in,present |
R4231 |
T6963 |
T6964 |
det |
the,syringe |
R4232 |
T6964 |
T6962 |
pobj |
syringe,in |
R4233 |
T6965 |
T6944 |
punct |
.,inhibited |
R4234 |
T6967 |
T6968 |
det |
The,mice |
R4235 |
T6968 |
T6969 |
nsubjpass |
mice,killed |
R4236 |
T6970 |
T6969 |
auxpass |
were,killed |
R4237 |
T6971 |
T6969 |
prep |
by,killed |
R4238 |
T6972 |
T6973 |
amod |
cervical,dislocation |
R4239 |
T6973 |
T6971 |
pobj |
dislocation,by |
R4240 |
T6974 |
T6969 |
prep |
in,killed |
R4241 |
T6975 |
T6974 |
pobj |
order,in |
R4242 |
T6976 |
T6977 |
aux |
to,avoid |
R4243 |
T6977 |
T6975 |
acl |
avoid,order |
R4244 |
T6978 |
T6979 |
det |
any,effect |
R4245 |
T6979 |
T6977 |
dobj |
effect,avoid |
R4246 |
T6980 |
T6979 |
prep |
of,effect |
R4247 |
T6981 |
T6982 |
amod |
anaesthetic,chemicals |
R4248 |
T6982 |
T6980 |
pobj |
chemicals,of |
R4249 |
T6983 |
T6979 |
prep |
on,effect |
R4250 |
T6984 |
T6985 |
compound |
blood,parameters |
R4251 |
T6985 |
T6983 |
pobj |
parameters,on |
R4252 |
T6986 |
T6969 |
punct |
.,killed |
R4253 |
T6988 |
T6989 |
det |
The,mice |
R4254 |
T6989 |
T6994 |
nsubjpass |
mice,matched |
R4255 |
T6990 |
T6991 |
amod |
wild,type |
R4256 |
T6991 |
T6989 |
nmod |
type,mice |
R4257 |
T6992 |
T6991 |
cc |
and,type |
R4258 |
T6993 |
T6991 |
conj |
mutant,type |
R4259 |
T6995 |
T6994 |
auxpass |
were,matched |
R4260 |
T6996 |
T6994 |
prep |
regarding,matched |
R4261 |
T6997 |
T6998 |
poss |
their,age |
R4262 |
T6998 |
T6996 |
pobj |
age,regarding |
R4263 |
T6999 |
T6998 |
punct |
", ",age |
R4264 |
T7000 |
T6998 |
conj |
weight,age |
R4265 |
T7001 |
T7000 |
cc |
and,weight |
R4266 |
T7002 |
T7000 |
conj |
sex,weight |
R4267 |
T7003 |
T6994 |
punct |
.,matched |
R4268 |
T7005 |
T7006 |
det |
The,daytime |
R4269 |
T7006 |
T7007 |
nsubjpass |
daytime,kept |
R4270 |
T7008 |
T7006 |
prep |
of,daytime |
R4271 |
T7009 |
T7010 |
compound |
blood,collection |
R4272 |
T7010 |
T7008 |
pobj |
collection,of |
R4273 |
T7011 |
T7006 |
punct |
", ",daytime |
R4274 |
T7012 |
T7013 |
amod |
sampling,site |
R4275 |
T7013 |
T7006 |
conj |
site,daytime |
R4276 |
T7014 |
T7013 |
cc |
and,site |
R4277 |
T7015 |
T7016 |
compound |
collection,method |
R4278 |
T7016 |
T7013 |
conj |
method,site |
R4279 |
T7017 |
T7007 |
auxpass |
were,kept |
R4280 |
T7018 |
T7007 |
oprd |
constant,kept |
R4281 |
T7019 |
T7007 |
prep |
during,kept |
R4282 |
T7020 |
T7021 |
det |
all,experiments |
R4283 |
T7021 |
T7019 |
pobj |
experiments,during |
R4284 |
T7022 |
T7007 |
punct |
.,kept |
R4285 |
T7024 |
T7025 |
det |
A,volume |
R4286 |
T7025 |
T7030 |
nsubj |
volume,consisted |
R4287 |
T7026 |
T7025 |
amod |
typical,volume |
R4288 |
T7027 |
T7028 |
amod |
murine,sample |
R4289 |
T7028 |
T7025 |
compound |
sample,volume |
R4290 |
T7029 |
T7028 |
compound |
blood,sample |
R4291 |
T7031 |
T7030 |
prep |
of,consisted |
R4292 |
T7032 |
T7033 |
nummod |
550,μl |
R4293 |
T7033 |
T7031 |
pobj |
μl,of |
R4294 |
T7034 |
T7030 |
punct |
", ",consisted |
R4295 |
T7035 |
T7030 |
prep |
with,consisted |
R4296 |
T7036 |
T7035 |
pobj |
variations,with |
R4297 |
T7037 |
T7036 |
acl |
ranging,variations |
R4298 |
T7038 |
T7037 |
prep |
from,ranging |
R4299 |
T7039 |
T7040 |
nummod |
200,μl |
R4300 |
T7040 |
T7038 |
pobj |
μl,from |
R4301 |
T7041 |
T7038 |
prep |
to,from |
R4302 |
T7042 |
T7043 |
nummod |
900,μl |
R4303 |
T7043 |
T7041 |
pobj |
μl,to |
R4304 |
T7044 |
T7030 |
punct |
.,consisted |
R4305 |
T7046 |
T7047 |
amod |
Human,samples |
R4306 |
T7047 |
T7049 |
nsubjpass |
samples,collected |
R4307 |
T7048 |
T7047 |
compound |
blood,samples |
R4308 |
T7050 |
T7047 |
prep |
from,samples |
R4309 |
T7051 |
T7052 |
det |
the,authors |
R4310 |
T7052 |
T7050 |
pobj |
authors,from |
R4311 |
T7053 |
T7049 |
auxpass |
were,collected |
R4312 |
T7054 |
T7049 |
prep |
by,collected |
R4313 |
T7055 |
T7056 |
compound |
vein,puncture |
R4314 |
T7056 |
T7054 |
pobj |
puncture,by |
R4315 |
T7057 |
T7049 |
advcl |
using,collected |
R4316 |
T7058 |
T7059 |
amod |
common,monovettes |
R4317 |
T7059 |
T7057 |
dobj |
monovettes,using |
R4318 |
T7060 |
T7059 |
compound |
EDTA,monovettes |
R4319 |
T7061 |
T7059 |
punct |
-,monovettes |
R4320 |
T7062 |
T7063 |
punct |
(,Sarstedt |
R4321 |
T7063 |
T7049 |
parataxis |
Sarstedt,collected |
R4322 |
T7064 |
T7063 |
punct |
),Sarstedt |
R4323 |
T7065 |
T7049 |
punct |
.,collected |
R4324 |
T7067 |
T7068 |
nsubj |
We,performed |
R4325 |
T7069 |
T7070 |
poss |
our,studies |
R4326 |
T7070 |
T7068 |
dobj |
studies,performed |
R4327 |
T7071 |
T7068 |
advcl |
using,performed |
R4328 |
T7072 |
T7073 |
advmod |
well,standardized |
R4329 |
T7073 |
T7074 |
amod |
standardized,conditions |
R4330 |
T7074 |
T7071 |
dobj |
conditions,using |
R4331 |
T7075 |
T7074 |
compound |
sampling,conditions |
R4332 |
T7076 |
T7068 |
punct |
", ",performed |
R4333 |
T7077 |
T7078 |
mark |
as,show |
R4334 |
T7078 |
T7068 |
advcl |
show,performed |
R4335 |
T7079 |
T7078 |
nsubj |
differences,show |
R4336 |
T7080 |
T7079 |
prep |
in,differences |
R4337 |
T7081 |
T7082 |
det |
the,site |
R4338 |
T7082 |
T7080 |
pobj |
site,in |
R4339 |
T7083 |
T7084 |
compound |
blood,sampling |
R4340 |
T7084 |
T7082 |
compound |
sampling,site |
R4341 |
T7085 |
T7079 |
punct |
", ",differences |
R4342 |
T7086 |
T7087 |
prep |
for,between |
R4343 |
T7087 |
T7079 |
prep |
between,differences |
R4344 |
T7088 |
T7086 |
pobj |
example,for |
R4345 |
T7089 |
T7087 |
pobj |
tail,between |
R4346 |
T7090 |
T7089 |
cc |
and,tail |
R4347 |
T7091 |
T7089 |
conj |
heart,tail |
R4348 |
T7092 |
T7078 |
punct |
", ",show |
R4349 |
T7093 |
T7094 |
amod |
large,changes |
R4350 |
T7094 |
T7078 |
dobj |
changes,show |
R4351 |
T7095 |
T7094 |
prep |
in,changes |
R4352 |
T7096 |
T7097 |
det |
all,counts |
R4353 |
T7097 |
T7095 |
pobj |
counts,in |
R4354 |
T7098 |
T7099 |
compound |
cell,type |
R4355 |
T7099 |
T7097 |
compound |
type,counts |
R4356 |
T7100 |
T7068 |
punct |
.,performed |
R4357 |
T7102 |
T7103 |
amod |
First,draw |
R4358 |
T7103 |
T7107 |
nsubj |
draw,affect |
R4359 |
T7104 |
T7102 |
cc |
and,First |
R4360 |
T7105 |
T7102 |
conj |
second,First |
R4361 |
T7106 |
T7103 |
compound |
sample,draw |
R4362 |
T7108 |
T7109 |
compound |
blood,measurements |
R4363 |
T7109 |
T7107 |
dobj |
measurements,affect |
R4364 |
T7110 |
T7107 |
punct |
", ",affect |
R4365 |
T7111 |
T7107 |
advmod |
too,affect |
R4366 |
T7112 |
T7113 |
punct |
[,22 |
R4367 |
T7113 |
T7107 |
parataxis |
22,affect |
R4368 |
T7114 |
T7113 |
punct |
],22 |
R4369 |
T7115 |
T7107 |
punct |
.,affect |
R4370 |
T7117 |
T7118 |
advmod |
Also,affects |
R4371 |
T7119 |
T7120 |
det |
the,background |
R4372 |
T7120 |
T7118 |
nsubj |
background,affects |
R4373 |
T7121 |
T7120 |
amod |
genetic,background |
R4374 |
T7122 |
T7120 |
prep |
in,background |
R4375 |
T7123 |
T7124 |
amod |
common,mice |
R4376 |
T7124 |
T7122 |
pobj |
mice,in |
R4377 |
T7125 |
T7124 |
nmod |
laboratory,mice |
R4378 |
T7126 |
T7125 |
cc |
and,laboratory |
R4379 |
T7127 |
T7125 |
conj |
transgenic,laboratory |
R4380 |
T7128 |
T7129 |
det |
the,phenotype |
R4381 |
T7129 |
T7118 |
dobj |
phenotype,affects |
R4382 |
T7130 |
T7118 |
punct |
.,affects |
R4383 |
T7132 |
T7133 |
det |
A,change |
R4384 |
T7133 |
T7134 |
nsubj |
change,protect |
R4385 |
T7135 |
T7133 |
prep |
from,change |
R4386 |
T7136 |
T7137 |
nummod |
one,strain |
R4387 |
T7137 |
T7135 |
pobj |
strain,from |
R4388 |
T7138 |
T7133 |
prep |
to,change |
R4389 |
T7139 |
T7138 |
pobj |
another,to |
R4390 |
T7140 |
T7134 |
aux |
may,protect |
R4391 |
T7141 |
T7134 |
dobj |
mice,protect |
R4392 |
T7142 |
T7134 |
prep |
from,protect |
R4393 |
T7143 |
T7142 |
pobj |
effects,from |
R4394 |
T7144 |
T7143 |
prep |
of,effects |
R4395 |
T7145 |
T7146 |
det |
the,defect |
R4396 |
T7146 |
T7144 |
pobj |
defect,of |
R4397 |
T7147 |
T7148 |
advmod |
primarily,induced |
R4398 |
T7148 |
T7146 |
amod |
induced,defect |
R4399 |
T7149 |
T7146 |
amod |
genetic,defect |
R4400 |
T7150 |
T7134 |
punct |
.,protect |
R4401 |
T7152 |
T7153 |
prep |
For,have |
R4402 |
T7154 |
T7152 |
pobj |
example,For |
R4403 |
T7155 |
T7153 |
punct |
", ",have |
R4404 |
T7156 |
T7157 |
compound |
BALB,c |
R4405 |
T7157 |
T7159 |
compound |
c,mice |
R4406 |
T7158 |
T7157 |
punct |
/,c |
R4407 |
T7159 |
T7153 |
nsubj |
mice,have |
R4408 |
T7160 |
T7161 |
det |
a,velocity |
R4409 |
T7161 |
T7153 |
dobj |
velocity,have |
R4410 |
T7162 |
T7163 |
advmod |
significantly,increased |
R4411 |
T7163 |
T7161 |
amod |
increased,velocity |
R4412 |
T7164 |
T7161 |
prep |
of,velocity |
R4413 |
T7165 |
T7166 |
compound |
platelet,aggregation |
R4414 |
T7166 |
T7164 |
pobj |
aggregation,of |
R4415 |
T7167 |
T7153 |
prep |
as,have |
R4416 |
T7168 |
T7167 |
prep |
compared,as |
R4417 |
T7169 |
T7168 |
prep |
to,compared |
R4418 |
T7170 |
T7171 |
compound |
129Sv,mice |
R4419 |
T7171 |
T7169 |
pobj |
mice,to |
R4420 |
T7172 |
T7171 |
punct |
", ",mice |
R4421 |
T7173 |
T7174 |
dep |
which,used |
R4422 |
T7174 |
T7171 |
relcl |
used,mice |
R4423 |
T7175 |
T7174 |
auxpass |
were,used |
R4424 |
T7176 |
T7174 |
prep |
throughout,used |
R4425 |
T7177 |
T7178 |
det |
this,study |
R4426 |
T7178 |
T7176 |
pobj |
study,throughout |
R4427 |
T7179 |
T7180 |
punct |
[,23 |
R4428 |
T7180 |
T7153 |
parataxis |
23,have |
R4429 |
T7181 |
T7180 |
punct |
],23 |
R4430 |
T7182 |
T7153 |
punct |
.,have |
R4431 |
T7273 |
T7274 |
npadvmod |
Self,forming |
R4432 |
T7274 |
T7275 |
amod |
forming,gradients |
R4433 |
T7276 |
T7275 |
compound |
iodixanol,gradients |
R4434 |
T7277 |
T7275 |
compound |
density,gradients |
R4435 |
T7278 |
T7275 |
prep |
of,gradients |
R4436 |
T7279 |
T7280 |
amod |
human,cells |
R4437 |
T7280 |
T7278 |
pobj |
cells,of |
R4438 |
T7281 |
T7280 |
amod |
red,cells |
R4439 |
T7282 |
T7280 |
compound |
blood,cells |
R4440 |
T7284 |
T7285 |
det |
The,distribution |
R4441 |
T7285 |
T7287 |
nsubjpass |
distribution,addressed |
R4442 |
T7286 |
T7285 |
amod |
subcellular,distribution |
R4443 |
T7288 |
T7285 |
prep |
of,distribution |
R4444 |
T7289 |
T7290 |
compound |
annexin,A7 |
R4445 |
T7290 |
T7288 |
pobj |
A7,of |
R4446 |
T7291 |
T7287 |
auxpass |
was,addressed |
R4447 |
T7292 |
T7287 |
prep |
according,addressed |
R4448 |
T7293 |
T7292 |
prep |
to,according |
R4449 |
T7294 |
T7293 |
punct |
[,to |
R4450 |
T7295 |
T7293 |
pobj |
24,to |
R4451 |
T7296 |
T7287 |
punct |
],addressed |
R4452 |
T7297 |
T7287 |
punct |
.,addressed |
R4453 |
T7299 |
T7300 |
amod |
Red,cells |
R4454 |
T7300 |
T7302 |
nsubjpass |
cells,centrifuged |
R4455 |
T7301 |
T7300 |
compound |
blood,cells |
R4456 |
T7303 |
T7302 |
auxpass |
were,centrifuged |
R4457 |
T7304 |
T7302 |
cc |
and,centrifuged |
R4458 |
T7305 |
T7302 |
conj |
washed,centrifuged |
R4459 |
T7306 |
T7305 |
prep |
in,washed |
R4460 |
T7307 |
T7308 |
amod |
isotonic,solution |
R4461 |
T7308 |
T7306 |
pobj |
solution,in |
R4462 |
T7309 |
T7310 |
amod |
buffered,salt |
R4463 |
T7310 |
T7308 |
compound |
salt,solution |
R4464 |
T7311 |
T7305 |
punct |
", ",washed |
R4465 |
T7312 |
T7305 |
conj |
collected,washed |
R4466 |
T7313 |
T7312 |
prep |
in,collected |
R4467 |
T7314 |
T7315 |
npadvmod |
ice,cold |
R4468 |
T7315 |
T7316 |
amod |
cold,buffer |
R4469 |
T7316 |
T7313 |
pobj |
buffer,in |
R4470 |
T7317 |
T7316 |
compound |
lysis,buffer |
R4471 |
T7318 |
T7319 |
punct |
(,Hepes |
R4472 |
T7319 |
T7316 |
parataxis |
Hepes,buffer |
R4473 |
T7320 |
T7321 |
nummod |
20,mM |
R4474 |
T7321 |
T7319 |
compound |
mM,Hepes |
R4475 |
T7322 |
T7319 |
punct |
", ",Hepes |
R4476 |
T7323 |
T7319 |
npadvmod |
pH,Hepes |
R4477 |
T7324 |
T7323 |
nummod |
7.4,pH |
R4478 |
T7325 |
T7319 |
punct |
", ",Hepes |
R4479 |
T7326 |
T7327 |
nummod |
200,μM |
R4480 |
T7327 |
T7328 |
compound |
μM,CaCl2 |
R4481 |
T7328 |
T7319 |
conj |
CaCl2,Hepes |
R4482 |
T7329 |
T7328 |
punct |
", ",CaCl2 |
R4483 |
T7330 |
T7331 |
nummod |
0.25,M |
R4484 |
T7331 |
T7332 |
compound |
M,sucrose |
R4485 |
T7332 |
T7328 |
conj |
sucrose,CaCl2 |
R4486 |
T7333 |
T7332 |
punct |
", ",sucrose |
R4487 |
T7334 |
T7332 |
cc |
and,sucrose |
R4488 |
T7335 |
T7336 |
compound |
proteinase,inhibitors |
R4489 |
T7336 |
T7332 |
conj |
inhibitors,sucrose |
R4490 |
T7337 |
T7338 |
punct |
(,Roche |
R4491 |
T7338 |
T7336 |
parataxis |
Roche,inhibitors |
R4492 |
T7339 |
T7338 |
punct |
),Roche |
R4493 |
T7340 |
T7336 |
punct |
),inhibitors |
R4494 |
T7341 |
T7312 |
punct |
", ",collected |
R4495 |
T7342 |
T7312 |
conj |
homogenized,collected |
R4496 |
T7343 |
T7342 |
prep |
by,homogenized |
R4497 |
T7344 |
T7345 |
det |
a,homogenizer |
R4498 |
T7345 |
T7343 |
pobj |
homogenizer,by |
R4499 |
T7346 |
T7347 |
advmod |
loosely,fitting |
R4500 |
T7347 |
T7345 |
amod |
fitting,homogenizer |
R4501 |
T7348 |
T7345 |
compound |
dounce,homogenizer |
R4502 |
T7349 |
T7345 |
cc |
and,homogenizer |
R4503 |
T7350 |
T7351 |
amod |
additional,passages |
R4504 |
T7351 |
T7345 |
conj |
passages,homogenizer |
R4505 |
T7352 |
T7351 |
prep |
through,passages |
R4506 |
T7353 |
T7354 |
det |
a,needle |
R4507 |
T7354 |
T7352 |
pobj |
needle,through |
R4508 |
T7355 |
T7356 |
nummod |
22,gauge |
R4509 |
T7356 |
T7354 |
compound |
gauge,needle |
R4510 |
T7357 |
T7302 |
punct |
.,centrifuged |
R4511 |
T7359 |
T7360 |
det |
A,solution |
R4512 |
T7360 |
T7364 |
nsubjpass |
solution,established |
R4513 |
T7361 |
T7362 |
nummod |
50,% |
R4514 |
T7362 |
T7360 |
compound |
%,solution |
R4515 |
T7363 |
T7360 |
compound |
iodixanol,solution |
R4516 |
T7365 |
T7364 |
auxpass |
was,established |
R4517 |
T7366 |
T7364 |
prep |
by,established |
R4518 |
T7367 |
T7366 |
pcomp |
mixing,by |
R4519 |
T7368 |
T7369 |
compound |
Optiprep,solution |
R4520 |
T7369 |
T7367 |
dobj |
solution,mixing |
R4521 |
T7370 |
T7371 |
punct |
(,Sigma |
R4522 |
T7371 |
T7369 |
parataxis |
Sigma,solution |
R4523 |
T7372 |
T7373 |
nummod |
60,% |
R4524 |
T7373 |
T7374 |
compound |
%,iodixanol |
R4525 |
T7374 |
T7371 |
dep |
iodixanol,Sigma |
R4526 |
T7375 |
T7371 |
punct |
;,Sigma |
R4527 |
T7376 |
T7371 |
punct |
),Sigma |
R4528 |
T7377 |
T7367 |
prep |
with,mixing |
R4529 |
T7378 |
T7377 |
pobj |
buffer,with |
R4530 |
T7379 |
T7380 |
punct |
(,sucrose |
R4531 |
T7380 |
T7378 |
parataxis |
sucrose,buffer |
R4532 |
T7381 |
T7382 |
nummod |
120,mM |
R4533 |
T7382 |
T7383 |
compound |
mM,Hepes |
R4534 |
T7383 |
T7380 |
dep |
Hepes,sucrose |
R4535 |
T7384 |
T7383 |
punct |
", ",Hepes |
R4536 |
T7385 |
T7383 |
npadvmod |
pH,Hepes |
R4537 |
T7386 |
T7385 |
nummod |
7.4,pH |
R4538 |
T7387 |
T7380 |
punct |
", ",sucrose |
R4539 |
T7388 |
T7389 |
nummod |
1.2,mM |
R4540 |
T7389 |
T7390 |
compound |
mM,CaCl2 |
R4541 |
T7390 |
T7380 |
dep |
CaCl2,sucrose |
R4542 |
T7391 |
T7380 |
punct |
", ",sucrose |
R4543 |
T7392 |
T7393 |
nummod |
0.25,M |
R4544 |
T7393 |
T7380 |
compound |
M,sucrose |
R4545 |
T7394 |
T7380 |
punct |
),sucrose |
R4546 |
T7395 |
T7364 |
punct |
.,established |
R4547 |
T7397 |
T7398 |
nummod |
Seven,parts |
R4548 |
T7398 |
T7399 |
nsubjpass |
parts,mixed |
R4549 |
T7400 |
T7398 |
prep |
of,parts |
R4550 |
T7401 |
T7402 |
det |
this,solution |
R4551 |
T7402 |
T7400 |
pobj |
solution,of |
R4552 |
T7403 |
T7404 |
nummod |
50,% |
R4553 |
T7404 |
T7402 |
compound |
%,solution |
R4554 |
T7405 |
T7402 |
compound |
iodixanol,solution |
R4555 |
T7406 |
T7399 |
auxpass |
were,mixed |
R4556 |
T7407 |
T7399 |
prep |
with,mixed |
R4557 |
T7408 |
T7409 |
nummod |
thirteen,parts |
R4558 |
T7409 |
T7407 |
pobj |
parts,with |
R4559 |
T7410 |
T7409 |
prep |
of,parts |
R4560 |
T7411 |
T7412 |
det |
the,solution |
R4561 |
T7412 |
T7410 |
pobj |
solution,of |
R4562 |
T7413 |
T7414 |
amod |
red,cell |
R4563 |
T7414 |
T7412 |
compound |
cell,solution |
R4564 |
T7415 |
T7414 |
compound |
blood,cell |
R4565 |
T7416 |
T7399 |
punct |
", ",mixed |
R4566 |
T7417 |
T7399 |
advcl |
generating,mixed |
R4567 |
T7418 |
T7419 |
det |
a,solution |
R4568 |
T7419 |
T7417 |
dobj |
solution,generating |
R4569 |
T7420 |
T7419 |
compound |
gradient,solution |
R4570 |
T7421 |
T7419 |
acl |
containing,solution |
R4571 |
T7422 |
T7423 |
nummod |
17.5,% |
R4572 |
T7423 |
T7424 |
compound |
%,iodixanol |
R4573 |
T7424 |
T7421 |
dobj |
iodixanol,containing |
R4574 |
T7425 |
T7399 |
punct |
.,mixed |
R4575 |
T7427 |
T7428 |
det |
The,mixture |
R4576 |
T7428 |
T7429 |
nsubj |
mixture,filled |
R4577 |
T7430 |
T7429 |
aux |
was,filled |
R4578 |
T7431 |
T7429 |
prep |
into,filled |
R4579 |
T7432 |
T7433 |
det |
a,tube |
R4580 |
T7433 |
T7431 |
pobj |
tube,into |
R4581 |
T7434 |
T7433 |
amod |
centrifuge,tube |
R4582 |
T7435 |
T7433 |
amod |
underlayed,tube |
R4583 |
T7436 |
T7435 |
prep |
by,underlayed |
R4584 |
T7437 |
T7436 |
pobj |
cushions,by |
R4585 |
T7438 |
T7437 |
prep |
of,cushions |
R4586 |
T7439 |
T7440 |
nummod |
30,% |
R4587 |
T7440 |
T7441 |
nmod |
%,iodixanol |
R4588 |
T7441 |
T7438 |
pobj |
iodixanol,of |
R4589 |
T7442 |
T7440 |
cc |
and,% |
R4590 |
T7443 |
T7444 |
nummod |
35,% |
R4591 |
T7444 |
T7440 |
conj |
%,% |
R4592 |
T7445 |
T7429 |
cc |
and,filled |
R4593 |
T7446 |
T7429 |
conj |
centrifuged,filled |
R4594 |
T7447 |
T7446 |
prep |
for,centrifuged |
R4595 |
T7448 |
T7449 |
nummod |
3,hours |
R4596 |
T7449 |
T7447 |
pobj |
hours,for |
R4597 |
T7450 |
T7446 |
prep |
at,centrifuged |
R4598 |
T7451 |
T7452 |
nummod |
"270,000",g |
R4599 |
T7452 |
T7450 |
pobj |
g,at |
R4600 |
T7453 |
T7452 |
punct |
×,g |
R4601 |
T7454 |
T7446 |
prep |
in,centrifuged |
R4602 |
T7455 |
T7456 |
det |
a,rotor |
R4603 |
T7456 |
T7454 |
pobj |
rotor,in |
R4604 |
T7457 |
T7458 |
compound |
SW41,Ti |
R4605 |
T7458 |
T7456 |
compound |
Ti,rotor |
R4606 |
T7459 |
T7460 |
compound |
swing,out |
R4607 |
T7460 |
T7456 |
compound |
out,rotor |
R4608 |
T7461 |
T7462 |
punct |
(,Beckman |
R4609 |
T7462 |
T7456 |
parataxis |
Beckman,rotor |
R4610 |
T7463 |
T7462 |
punct |
),Beckman |
R4611 |
T7464 |
T7465 |
aux |
to,achieve |
R4612 |
T7465 |
T7429 |
advcl |
achieve,filled |
R4613 |
T7466 |
T7467 |
det |
a,gradient |
R4614 |
T7467 |
T7465 |
dobj |
gradient,achieve |
R4615 |
T7468 |
T7469 |
advmod |
nearly,linear |
R4616 |
T7469 |
T7467 |
amod |
linear,gradient |
R4617 |
T7470 |
T7467 |
compound |
density,gradient |
R4618 |
T7471 |
T7472 |
punct |
(,g |
R4619 |
T7472 |
T7467 |
parataxis |
g,gradient |
R4620 |
T7473 |
T7472 |
advmod |
approximately,g |
R4621 |
T7474 |
T7472 |
nummod |
1.06,g |
R4622 |
T7475 |
T7476 |
punct |
/,ml |
R4623 |
T7476 |
T7472 |
prep |
ml,g |
R4624 |
T7477 |
T7472 |
prep |
to,g |
R4625 |
T7478 |
T7479 |
nummod |
1.20,g |
R4626 |
T7479 |
T7477 |
pobj |
g,to |
R4627 |
T7480 |
T7481 |
punct |
/,ml |
R4628 |
T7481 |
T7479 |
prep |
ml,g |
R4629 |
T7482 |
T7472 |
punct |
),g |
R4630 |
T7483 |
T7484 |
punct |
[,24 |
R4631 |
T7484 |
T7429 |
parataxis |
24,filled |
R4632 |
T7485 |
T7484 |
punct |
],24 |
R4633 |
T7486 |
T7429 |
punct |
.,filled |
R4634 |
T7488 |
T7489 |
nsubjpass |
Fractions,collected |
R4635 |
T7490 |
T7488 |
prep |
of,Fractions |
R4636 |
T7491 |
T7492 |
nummod |
0.6,ml |
R4637 |
T7492 |
T7490 |
pobj |
ml,of |
R4638 |
T7493 |
T7489 |
auxpass |
were,collected |
R4639 |
T7494 |
T7489 |
prep |
from,collected |
R4640 |
T7495 |
T7496 |
det |
the,top |
R4641 |
T7496 |
T7494 |
pobj |
top,from |
R4642 |
T7497 |
T7496 |
prep |
of,top |
R4643 |
T7498 |
T7499 |
det |
the,gradient |
R4644 |
T7499 |
T7497 |
pobj |
gradient,of |
R4645 |
T7500 |
T7489 |
prep |
by,collected |
R4646 |
T7501 |
T7502 |
det |
a,syringe |
R4647 |
T7502 |
T7500 |
pobj |
syringe,by |
R4648 |
T7503 |
T7504 |
nummod |
1,ml |
R4649 |
T7504 |
T7502 |
compound |
ml,syringe |
R4650 |
T7505 |
T7489 |
prep |
without,collected |
R4651 |
T7506 |
T7505 |
pcomp |
using,without |
R4652 |
T7507 |
T7508 |
det |
a,needle |
R4653 |
T7508 |
T7506 |
dobj |
needle,using |
R4654 |
T7509 |
T7489 |
cc |
and,collected |
R4655 |
T7510 |
T7511 |
advmod |
immediately,frozen |
R4656 |
T7511 |
T7489 |
conj |
frozen,collected |
R4657 |
T7512 |
T7511 |
prep |
in,frozen |
R4658 |
T7513 |
T7514 |
amod |
liquid,nitrogen |
R4659 |
T7514 |
T7512 |
pobj |
nitrogen,in |
R4660 |
T7515 |
T7511 |
prep |
for,frozen |
R4661 |
T7516 |
T7517 |
amod |
further,use |
R4662 |
T7517 |
T7515 |
pobj |
use,for |
R4663 |
T7518 |
T7489 |
punct |
.,collected |
R4664 |
T7632 |
T7633 |
compound |
Exovesicle,generation |
R4665 |
T7634 |
T7633 |
prep |
from,generation |
R4666 |
T7635 |
T7636 |
amod |
human,cells |
R4667 |
T7636 |
T7634 |
pobj |
cells,from |
R4668 |
T7637 |
T7635 |
cc |
and,human |
R4669 |
T7638 |
T7635 |
conj |
murine,human |
R4670 |
T7639 |
T7636 |
amod |
red,cells |
R4671 |
T7640 |
T7636 |
compound |
blood,cells |
R4672 |
T7642 |
T7643 |
amod |
Red,cells |
R4673 |
T7643 |
T7645 |
nsubj |
cells,form |
R4674 |
T7644 |
T7643 |
compound |
blood,cells |
R4675 |
T7646 |
T7645 |
advmod |
naturally,form |
R4676 |
T7647 |
T7645 |
aux |
can,form |
R4677 |
T7648 |
T7649 |
npadvmod |
hemoglobin,containing |
R4678 |
T7649 |
T7650 |
amod |
containing,exovesicles |
R4679 |
T7650 |
T7645 |
dobj |
exovesicles,form |
R4680 |
T7651 |
T7645 |
punct |
.,form |
R4681 |
T7653 |
T7654 |
advmod |
In,vitro |
R4682 |
T7654 |
T7655 |
advmod |
vitro,is |
R4683 |
T7656 |
T7657 |
det |
these,formation |
R4684 |
T7657 |
T7655 |
nsubj |
formation,is |
R4685 |
T7658 |
T7657 |
compound |
vesicle,formation |
R4686 |
T7659 |
T7660 |
npadvmod |
Ca2+,induced |
R4687 |
T7660 |
T7655 |
acomp |
induced,is |
R4688 |
T7661 |
T7655 |
punct |
.,is |
R4689 |
T7663 |
T7664 |
advmod |
Freshly,collected |
R4690 |
T7664 |
T7665 |
amod |
collected,samples |
R4691 |
T7665 |
T7671 |
nsubjpass |
samples,centrifuged |
R4692 |
T7666 |
T7667 |
nmod |
lithium,heparin |
R4693 |
T7667 |
T7665 |
nmod |
heparin,samples |
R4694 |
T7668 |
T7667 |
punct |
-,heparin |
R4695 |
T7669 |
T7665 |
amod |
anticoagulated,samples |
R4696 |
T7670 |
T7665 |
compound |
blood,samples |
R4697 |
T7672 |
T7671 |
auxpass |
were,centrifuged |
R4698 |
T7673 |
T7671 |
prep |
for,centrifuged |
R4699 |
T7674 |
T7675 |
nummod |
5,minutes |
R4700 |
T7675 |
T7673 |
pobj |
minutes,for |
R4701 |
T7676 |
T7671 |
prep |
at,centrifuged |
R4702 |
T7677 |
T7678 |
nummod |
400,g |
R4703 |
T7678 |
T7676 |
pobj |
g,at |
R4704 |
T7679 |
T7678 |
punct |
×,g |
R4705 |
T7680 |
T7678 |
cc |
and,g |
R4706 |
T7681 |
T7682 |
nummod |
4,°C |
R4707 |
T7682 |
T7678 |
conj |
°C,g |
R4708 |
T7683 |
T7671 |
punct |
.,centrifuged |
R4709 |
T7685 |
T7686 |
det |
The,erythrocytes |
R4710 |
T7686 |
T7687 |
nsubjpass |
erythrocytes,washed |
R4711 |
T7688 |
T7687 |
auxpass |
were,washed |
R4712 |
T7689 |
T7690 |
nummod |
five,times |
R4713 |
T7690 |
T7687 |
npadvmod |
times,washed |
R4714 |
T7691 |
T7687 |
prep |
with,washed |
R4715 |
T7692 |
T7693 |
nummod |
20,mM |
R4716 |
T7693 |
T7694 |
compound |
mM,Hepes |
R4717 |
T7694 |
T7691 |
pobj |
Hepes,with |
R4718 |
T7695 |
T7694 |
punct |
", ",Hepes |
R4719 |
T7696 |
T7694 |
npadvmod |
pH,Hepes |
R4720 |
T7697 |
T7696 |
nummod |
7.5,pH |
R4721 |
T7698 |
T7694 |
punct |
", ",Hepes |
R4722 |
T7699 |
T7694 |
cc |
and,Hepes |
R4723 |
T7700 |
T7701 |
nummod |
150,mM |
R4724 |
T7701 |
T7702 |
compound |
mM,NaCl |
R4725 |
T7702 |
T7694 |
conj |
NaCl,Hepes |
R4726 |
T7703 |
T7704 |
punct |
(,buffer |
R4727 |
T7704 |
T7702 |
parataxis |
buffer,NaCl |
R4728 |
T7705 |
T7704 |
amod |
washing,buffer |
R4729 |
T7706 |
T7704 |
punct |
),buffer |
R4730 |
T7707 |
T7687 |
punct |
.,washed |
R4731 |
T7709 |
T7710 |
det |
A,volume |
R4732 |
T7710 |
T7711 |
nsubjpass |
volume,combined |
R4733 |
T7712 |
T7710 |
prep |
of,volume |
R4734 |
T7713 |
T7714 |
nummod |
500,μl |
R4735 |
T7714 |
T7715 |
nmod |
μl,cells |
R4736 |
T7715 |
T7712 |
pobj |
cells,of |
R4737 |
T7716 |
T7715 |
amod |
washed,cells |
R4738 |
T7717 |
T7715 |
amod |
red,cells |
R4739 |
T7718 |
T7715 |
compound |
blood,cells |
R4740 |
T7719 |
T7711 |
auxpass |
were,combined |
R4741 |
T7720 |
T7711 |
prep |
with,combined |
R4742 |
T7721 |
T7722 |
nummod |
3,ml |
R4743 |
T7722 |
T7720 |
pobj |
ml,with |
R4744 |
T7723 |
T7722 |
prep |
of,ml |
R4745 |
T7724 |
T7725 |
compound |
vesiculation,buffer |
R4746 |
T7725 |
T7723 |
pobj |
buffer,of |
R4747 |
T7726 |
T7727 |
punct |
(,CaCl2 |
R4748 |
T7727 |
T7725 |
parataxis |
CaCl2,buffer |
R4749 |
T7728 |
T7729 |
nummod |
20,mM |
R4750 |
T7729 |
T7730 |
compound |
mM,Hepes |
R4751 |
T7730 |
T7727 |
dep |
Hepes,CaCl2 |
R4752 |
T7731 |
T7730 |
punct |
", ",Hepes |
R4753 |
T7732 |
T7730 |
npadvmod |
pH,Hepes |
R4754 |
T7733 |
T7732 |
nummod |
7.5,pH |
R4755 |
T7734 |
T7727 |
punct |
", ",CaCl2 |
R4756 |
T7735 |
T7736 |
nummod |
150,mM |
R4757 |
T7736 |
T7737 |
compound |
mM,NaCl |
R4758 |
T7737 |
T7727 |
dep |
NaCl,CaCl2 |
R4759 |
T7738 |
T7727 |
punct |
", ",CaCl2 |
R4760 |
T7739 |
T7740 |
nummod |
1,mM |
R4761 |
T7740 |
T7727 |
compound |
mM,CaCl2 |
R4762 |
T7741 |
T7727 |
acl |
adjusted,CaCl2 |
R4763 |
T7742 |
T7741 |
prep |
to,adjusted |
R4764 |
T7743 |
T7744 |
nummod |
5,μM |
R4765 |
T7744 |
T7745 |
compound |
μM,A23187 |
R4766 |
T7745 |
T7742 |
pobj |
A23187,to |
R4767 |
T7746 |
T7745 |
compound |
Ca2+,A23187 |
R4768 |
T7747 |
T7745 |
compound |
ionophore,A23187 |
R4769 |
T7748 |
T7727 |
punct |
),CaCl2 |
R4770 |
T7749 |
T7711 |
cc |
and,combined |
R4771 |
T7750 |
T7711 |
conj |
incubated,combined |
R4772 |
T7751 |
T7750 |
prep |
for,incubated |
R4773 |
T7752 |
T7753 |
nummod |
30,minutes |
R4774 |
T7753 |
T7751 |
pobj |
minutes,for |
R4775 |
T7754 |
T7750 |
prep |
at,incubated |
R4776 |
T7755 |
T7756 |
nummod |
37,°C |
R4777 |
T7756 |
T7754 |
pobj |
°C,at |
R4778 |
T7757 |
T7711 |
punct |
.,combined |
R4779 |
T7759 |
T7760 |
prep |
After,added |
R4780 |
T7761 |
T7759 |
pobj |
incubation,After |
R4781 |
T7762 |
T7760 |
nsubjpass |
EDTA,added |
R4782 |
T7763 |
T7760 |
auxpass |
was,added |
R4783 |
T7764 |
T7760 |
prep |
to,added |
R4784 |
T7765 |
T7766 |
det |
the,solution |
R4785 |
T7766 |
T7764 |
pobj |
solution,to |
R4786 |
T7767 |
T7760 |
prep |
to,added |
R4787 |
T7768 |
T7769 |
det |
a,concentration |
R4788 |
T7769 |
T7767 |
pobj |
concentration,to |
R4789 |
T7770 |
T7769 |
amod |
final,concentration |
R4790 |
T7771 |
T7769 |
prep |
of,concentration |
R4791 |
T7772 |
T7773 |
nummod |
5,mM |
R4792 |
T7773 |
T7771 |
pobj |
mM,of |
R4793 |
T7774 |
T7760 |
cc |
and,added |
R4794 |
T7775 |
T7760 |
conj |
centrifuged,added |
R4795 |
T7776 |
T7775 |
prep |
for,centrifuged |
R4796 |
T7777 |
T7778 |
nummod |
5,minutes |
R4797 |
T7778 |
T7776 |
pobj |
minutes,for |
R4798 |
T7779 |
T7775 |
prep |
at,centrifuged |
R4799 |
T7780 |
T7781 |
nummod |
400,g |
R4800 |
T7781 |
T7779 |
pobj |
g,at |
R4801 |
T7782 |
T7781 |
punct |
×,g |
R4802 |
T7783 |
T7781 |
cc |
and,g |
R4803 |
T7784 |
T7785 |
nummod |
4,°C |
R4804 |
T7785 |
T7781 |
conj |
°C,g |
R4805 |
T7786 |
T7760 |
punct |
.,added |
R4806 |
T7788 |
T7789 |
det |
The,supernatant |
R4807 |
T7789 |
T7790 |
nsubjpass |
supernatant,collected |
R4808 |
T7791 |
T7790 |
auxpass |
was,collected |
R4809 |
T7792 |
T7790 |
cc |
and,collected |
R4810 |
T7793 |
T7790 |
conj |
centrifuged,collected |
R4811 |
T7794 |
T7793 |
prep |
for,centrifuged |
R4812 |
T7795 |
T7796 |
nummod |
20,minutes |
R4813 |
T7796 |
T7794 |
pobj |
minutes,for |
R4814 |
T7797 |
T7793 |
prep |
at,centrifuged |
R4815 |
T7798 |
T7799 |
nummod |
"15,000",g |
R4816 |
T7799 |
T7797 |
pobj |
g,at |
R4817 |
T7800 |
T7799 |
punct |
×,g |
R4818 |
T7801 |
T7799 |
cc |
and,g |
R4819 |
T7802 |
T7803 |
nummod |
4,°C |
R4820 |
T7803 |
T7799 |
conj |
°C,g |
R4821 |
T7804 |
T7790 |
punct |
.,collected |
R4822 |
T7806 |
T7807 |
det |
The,pellet |
R4823 |
T7807 |
T7809 |
nsubj |
pellet,resuspended |
R4824 |
T7808 |
T7807 |
amod |
resulting,pellet |
R4825 |
T7810 |
T7809 |
aux |
was,resuspended |
R4826 |
T7811 |
T7809 |
prep |
in,resuspended |
R4827 |
T7812 |
T7813 |
amod |
washing,buffer |
R4828 |
T7813 |
T7811 |
pobj |
buffer,in |
R4829 |
T7814 |
T7809 |
cc |
and,resuspended |
R4830 |
T7815 |
T7809 |
conj |
centrifuged,resuspended |
R4831 |
T7816 |
T7815 |
prep |
at,centrifuged |
R4832 |
T7817 |
T7818 |
nummod |
400,g |
R4833 |
T7818 |
T7816 |
pobj |
g,at |
R4834 |
T7819 |
T7818 |
punct |
×,g |
R4835 |
T7820 |
T7815 |
advmod |
again,centrifuged |
R4836 |
T7821 |
T7822 |
aux |
to,collect |
R4837 |
T7822 |
T7809 |
advcl |
collect,resuspended |
R4838 |
T7823 |
T7824 |
det |
a,supernatant |
R4839 |
T7824 |
T7822 |
dobj |
supernatant,collect |
R4840 |
T7825 |
T7824 |
acl |
enriched,supernatant |
R4841 |
T7826 |
T7825 |
prep |
with,enriched |
R4842 |
T7827 |
T7828 |
amod |
red,cell |
R4843 |
T7828 |
T7830 |
compound |
cell,microvesicles |
R4844 |
T7829 |
T7828 |
compound |
blood,cell |
R4845 |
T7830 |
T7826 |
pobj |
microvesicles,with |
R4846 |
T7831 |
T7809 |
punct |
.,resuspended |
R4847 |
T7833 |
T7834 |
det |
The,supernatant |
R4848 |
T7834 |
T7835 |
nsubjpass |
supernatant,centrifuged |
R4849 |
T7836 |
T7834 |
prep |
of,supernatant |
R4850 |
T7837 |
T7838 |
det |
the,step |
R4851 |
T7838 |
T7836 |
pobj |
step,of |
R4852 |
T7839 |
T7840 |
nummod |
"15,000",g |
R4853 |
T7840 |
T7838 |
compound |
g,step |
R4854 |
T7841 |
T7840 |
punct |
×,g |
R4855 |
T7842 |
T7835 |
auxpass |
was,centrifuged |
R4856 |
T7843 |
T7835 |
advmod |
further,centrifuged |
R4857 |
T7844 |
T7835 |
prep |
for,centrifuged |
R4858 |
T7845 |
T7846 |
nummod |
60,minutes |
R4859 |
T7846 |
T7844 |
pobj |
minutes,for |
R4860 |
T7847 |
T7835 |
prep |
at,centrifuged |
R4861 |
T7848 |
T7849 |
nummod |
"100,000",g |
R4862 |
T7849 |
T7847 |
pobj |
g,at |
R4863 |
T7850 |
T7849 |
punct |
×,g |
R4864 |
T7851 |
T7849 |
cc |
and,g |
R4865 |
T7852 |
T7853 |
nummod |
4,°C |
R4866 |
T7853 |
T7849 |
conj |
°C,g |
R4867 |
T7854 |
T7835 |
punct |
.,centrifuged |
R4868 |
T7856 |
T7857 |
det |
The,pellet |
R4869 |
T7857 |
T7859 |
nsubj |
pellet,resuspended |
R4870 |
T7858 |
T7857 |
amod |
final,pellet |
R4871 |
T7860 |
T7857 |
prep |
of,pellet |
R4872 |
T7861 |
T7862 |
det |
this,step |
R4873 |
T7862 |
T7860 |
pobj |
step,of |
R4874 |
T7863 |
T7859 |
aux |
was,resuspended |
R4875 |
T7864 |
T7859 |
prep |
in,resuspended |
R4876 |
T7865 |
T7866 |
amod |
washing,buffer |
R4877 |
T7866 |
T7864 |
pobj |
buffer,in |
R4878 |
T7867 |
T7859 |
cc |
and,resuspended |
R4879 |
T7868 |
T7859 |
conj |
centrifuged,resuspended |
R4880 |
T7869 |
T7868 |
prep |
at,centrifuged |
R4881 |
T7870 |
T7871 |
nummod |
"15,000",g |
R4882 |
T7871 |
T7869 |
pobj |
g,at |
R4883 |
T7872 |
T7871 |
punct |
×,g |
R4884 |
T7873 |
T7868 |
advmod |
again,centrifuged |
R4885 |
T7874 |
T7875 |
aux |
to,purify |
R4886 |
T7875 |
T7859 |
advcl |
purify,resuspended |
R4887 |
T7876 |
T7877 |
det |
a,supernatant |
R4888 |
T7877 |
T7875 |
dobj |
supernatant,purify |
R4889 |
T7878 |
T7877 |
acl |
enriched,supernatant |
R4890 |
T7879 |
T7878 |
prep |
in,enriched |
R4891 |
T7880 |
T7879 |
pobj |
nanovesicles,in |
R4892 |
T7881 |
T7875 |
prep |
according,purify |
R4893 |
T7882 |
T7881 |
prep |
to,according |
R4894 |
T7883 |
T7882 |
punct |
[,to |
R4895 |
T7884 |
T7885 |
nummod |
7,20 |
R4896 |
T7885 |
T7882 |
pobj |
20,to |
R4897 |
T7886 |
T7885 |
punct |
",",20 |
R4898 |
T7887 |
T7859 |
punct |
],resuspended |
R4899 |
T7888 |
T7859 |
punct |
.,resuspended |
R4900 |
T7890 |
T7891 |
det |
The,vesicles |
R4901 |
T7891 |
T7892 |
nsubjpass |
vesicles,assayed |
R4902 |
T7893 |
T7892 |
auxpass |
were,assayed |
R4903 |
T7894 |
T7892 |
prep |
for,assayed |
R4904 |
T7895 |
T7896 |
compound |
acetylcholine,esterase |
R4905 |
T7896 |
T7897 |
compound |
esterase,activity |
R4906 |
T7897 |
T7894 |
pobj |
activity,for |
R4907 |
T7898 |
T7892 |
cc |
and,assayed |
R4908 |
T7899 |
T7892 |
conj |
used,assayed |
R4909 |
T7900 |
T7899 |
prep |
for,used |
R4910 |
T7901 |
T7902 |
amod |
further,analysis |
R4911 |
T7902 |
T7900 |
pobj |
analysis,for |
R4912 |
T7903 |
T7892 |
punct |
.,assayed |
R4913 |
T7951 |
T7952 |
compound |
Acetylcholine,esterase |
R4914 |
T7952 |
T7953 |
compound |
esterase,assay |
R4915 |
T7955 |
T7956 |
det |
A,sample |
R4916 |
T7956 |
T7959 |
nsubjpass |
sample,mixed |
R4917 |
T7957 |
T7958 |
nummod |
30,μl |
R4918 |
T7958 |
T7956 |
compound |
μl,sample |
R4919 |
T7960 |
T7956 |
prep |
of,sample |
R4920 |
T7961 |
T7962 |
amod |
red,cell |
R4921 |
T7962 |
T7964 |
compound |
cell,vesicles |
R4922 |
T7963 |
T7962 |
compound |
blood,cell |
R4923 |
T7964 |
T7960 |
pobj |
vesicles,of |
R4924 |
T7965 |
T7959 |
auxpass |
was,mixed |
R4925 |
T7966 |
T7959 |
prep |
with,mixed |
R4926 |
T7967 |
T7968 |
det |
an,volume |
R4927 |
T7968 |
T7966 |
pobj |
volume,with |
R4928 |
T7969 |
T7968 |
amod |
equal,volume |
R4929 |
T7970 |
T7968 |
prep |
of,volume |
R4930 |
T7971 |
T7970 |
pobj |
buffer,of |
R4931 |
T7972 |
T7973 |
punct |
(,X |
R4932 |
T7973 |
T7971 |
parataxis |
X,buffer |
R4933 |
T7974 |
T7975 |
nummod |
20,mM |
R4934 |
T7975 |
T7976 |
compound |
mM,Hepes |
R4935 |
T7976 |
T7973 |
dep |
Hepes,X |
R4936 |
T7977 |
T7976 |
punct |
", ",Hepes |
R4937 |
T7978 |
T7976 |
npadvmod |
pH,Hepes |
R4938 |
T7979 |
T7978 |
nummod |
7.6,pH |
R4939 |
T7980 |
T7973 |
punct |
", ",X |
R4940 |
T7981 |
T7982 |
nummod |
150,mM |
R4941 |
T7982 |
T7983 |
compound |
mM,NaCl |
R4942 |
T7983 |
T7973 |
dep |
NaCl,X |
R4943 |
T7984 |
T7973 |
punct |
", ",X |
R4944 |
T7985 |
T7986 |
nummod |
0.5,% |
R4945 |
T7986 |
T7973 |
compound |
%,X |
R4946 |
T7987 |
T7973 |
compound |
Triton,X |
R4947 |
T7988 |
T7973 |
punct |
-,X |
R4948 |
T7989 |
T7973 |
nummod |
100,X |
R4949 |
T7990 |
T7973 |
punct |
),X |
R4950 |
T7991 |
T7959 |
punct |
", ",mixed |
R4951 |
T7992 |
T7959 |
conj |
vortexed,mixed |
R4952 |
T7993 |
T7992 |
cc |
and,vortexed |
R4953 |
T7994 |
T7992 |
conj |
incubated,vortexed |
R4954 |
T7995 |
T7994 |
prep |
for,incubated |
R4955 |
T7996 |
T7997 |
nummod |
5,minutes |
R4956 |
T7997 |
T7995 |
pobj |
minutes,for |
R4957 |
T7998 |
T7994 |
prep |
at,incubated |
R4958 |
T7999 |
T8000 |
nummod |
37,°C |
R4959 |
T8000 |
T7998 |
pobj |
°C,at |
R4960 |
T8001 |
T7959 |
punct |
.,mixed |
R4961 |
T8003 |
T8004 |
advmod |
Subsequently,added |
R4962 |
T8005 |
T8006 |
nummod |
640,μl |
R4963 |
T8006 |
T8007 |
compound |
μl,buffer |
R4964 |
T8007 |
T8004 |
nsubjpass |
buffer,added |
R4965 |
T8008 |
T8009 |
compound |
sodium,phosphate |
R4966 |
T8009 |
T8007 |
compound |
phosphate,buffer |
R4967 |
T8010 |
T8011 |
punct |
(,Na2PO4 |
R4968 |
T8011 |
T8007 |
parataxis |
Na2PO4,buffer |
R4969 |
T8012 |
T8013 |
nummod |
100,mM |
R4970 |
T8013 |
T8011 |
compound |
mM,Na2PO4 |
R4971 |
T8014 |
T8011 |
punct |
", ",Na2PO4 |
R4972 |
T8015 |
T8011 |
npadvmod |
pH,Na2PO4 |
R4973 |
T8016 |
T8015 |
nummod |
7.6,pH |
R4974 |
T8017 |
T8011 |
punct |
),Na2PO4 |
R4975 |
T8018 |
T8007 |
punct |
", ",buffer |
R4976 |
T8019 |
T8020 |
nummod |
50,μl |
R4977 |
T8020 |
T8021 |
compound |
μl,DTNB |
R4978 |
T8021 |
T8007 |
conj |
DTNB,buffer |
R4979 |
T8022 |
T8023 |
punct |
(,mM |
R4980 |
T8023 |
T8021 |
parataxis |
mM,DTNB |
R4981 |
T8024 |
T8025 |
nummod |
5,dithiobis |
R4982 |
T8025 |
T8023 |
dep |
dithiobis,mM |
R4983 |
T8026 |
T8025 |
punct |
",",dithiobis |
R4984 |
T8027 |
T8025 |
nummod |
5,dithiobis |
R4985 |
T8028 |
T8027 |
punct |
',5 |
R4986 |
T8029 |
T8025 |
punct |
-,dithiobis |
R4987 |
T8030 |
T8023 |
punct |
-,mM |
R4988 |
T8031 |
T8032 |
punct |
(,acid |
R4989 |
T8032 |
T8023 |
parataxis |
acid,mM |
R4990 |
T8033 |
T8032 |
nummod |
2,acid |
R4991 |
T8034 |
T8032 |
punct |
-,acid |
R4992 |
T8035 |
T8032 |
amod |
nitrobenzoic,acid |
R4993 |
T8036 |
T8032 |
punct |
),acid |
R4994 |
T8037 |
T8023 |
punct |
", ",mM |
R4995 |
T8038 |
T8023 |
nummod |
10,mM |
R4996 |
T8039 |
T8023 |
prep |
in,mM |
R4997 |
T8040 |
T8041 |
compound |
sodium,phosphate |
R4998 |
T8041 |
T8042 |
compound |
phosphate,buffer |
R4999 |
T8042 |
T8039 |
pobj |
buffer,in |
R5000 |
T8043 |
T8023 |
punct |
),mM |
R5001 |
T8044 |
T8021 |
punct |
", ",DTNB |
R5002 |
T8045 |
T8021 |
cc |
and,DTNB |
R5003 |
T8046 |
T8047 |
nummod |
50,μl |
R5004 |
T8047 |
T8048 |
compound |
μl,chloride |
R5005 |
T8048 |
T8021 |
conj |
chloride,DTNB |
R5006 |
T8049 |
T8048 |
compound |
acetylthiocholine,chloride |
R5007 |
T8050 |
T8051 |
punct |
(,mM |
R5008 |
T8051 |
T8048 |
parataxis |
mM,chloride |
R5009 |
T8052 |
T8051 |
nummod |
12,mM |
R5010 |
T8053 |
T8051 |
punct |
",",mM |
R5011 |
T8054 |
T8051 |
nummod |
5,mM |
R5012 |
T8055 |
T8051 |
prep |
in,mM |
R5013 |
T8056 |
T8055 |
pobj |
H2O,in |
R5014 |
T8057 |
T8051 |
punct |
),mM |
R5015 |
T8058 |
T8004 |
auxpass |
were,added |
R5016 |
T8059 |
T8004 |
punct |
.,added |
R5017 |
T8061 |
T8062 |
det |
The,reaction |
R5018 |
T8062 |
T8063 |
nsubj |
reaction,took |
R5019 |
T8064 |
T8063 |
dobj |
place,took |
R5020 |
T8065 |
T8063 |
prep |
at,took |
R5021 |
T8066 |
T8067 |
compound |
room,temperature |
R5022 |
T8067 |
T8065 |
pobj |
temperature,at |
R5023 |
T8068 |
T8063 |
cc |
and,took |
R5024 |
T8069 |
T8070 |
auxpass |
was,measured |
R5025 |
T8070 |
T8063 |
conj |
measured,took |
R5026 |
T8071 |
T8070 |
advmod |
photometrically,measured |
R5027 |
T8072 |
T8070 |
prep |
at,measured |
R5028 |
T8073 |
T8074 |
det |
a,wavelength |
R5029 |
T8074 |
T8072 |
pobj |
wavelength,at |
R5030 |
T8075 |
T8074 |
prep |
of,wavelength |
R5031 |
T8076 |
T8077 |
nummod |
405,nm |
R5032 |
T8077 |
T8075 |
pobj |
nm,of |
R5033 |
T8078 |
T8070 |
prep |
according,measured |
R5034 |
T8079 |
T8078 |
prep |
to,according |
R5035 |
T8080 |
T8081 |
det |
the,method |
R5036 |
T8081 |
T8079 |
pobj |
method,to |
R5037 |
T8082 |
T8081 |
nmod |
Ellmann,method |
R5038 |
T8083 |
T8081 |
case |
',method |
R5039 |
T8084 |
T8085 |
punct |
[,25 |
R5040 |
T8085 |
T8070 |
parataxis |
25,measured |
R5041 |
T8086 |
T8085 |
punct |
],25 |
R5042 |
T8087 |
T8063 |
punct |
.,took |
R5043 |
T8114 |
T8113 |
prep |
of,Determination |
R5044 |
T8115 |
T8116 |
det |
the,resistance |
R5045 |
T8116 |
T8114 |
pobj |
resistance,of |
R5046 |
T8117 |
T8116 |
amod |
osmotic,resistance |
R5047 |
T8118 |
T8116 |
prep |
of,resistance |
R5048 |
T8119 |
T8120 |
amod |
red,cells |
R5049 |
T8120 |
T8118 |
pobj |
cells,of |
R5050 |
T8121 |
T8120 |
compound |
blood,cells |
R5051 |
T8123 |
T8124 |
det |
A,gradient |
R5052 |
T8124 |
T8130 |
nsubjpass |
gradient,used |
R5053 |
T8125 |
T8126 |
compound |
Na2HPO4,NaH2PO4 |
R5054 |
T8126 |
T8128 |
npadvmod |
NaH2PO4,buffered |
R5055 |
T8127 |
T8126 |
punct |
/,NaH2PO4 |
R5056 |
T8128 |
T8124 |
amod |
buffered,gradient |
R5057 |
T8129 |
T8124 |
compound |
NaCl,gradient |
R5058 |
T8131 |
T8132 |
punct |
(,pH |
R5059 |
T8132 |
T8124 |
npadvmod |
pH,gradient |
R5060 |
T8133 |
T8132 |
nummod |
7.4,pH |
R5061 |
T8134 |
T8132 |
punct |
),pH |
R5062 |
T8135 |
T8124 |
acl |
comprised,gradient |
R5063 |
T8136 |
T8135 |
prep |
of,comprised |
R5064 |
T8137 |
T8138 |
det |
the,steps |
R5065 |
T8138 |
T8136 |
pobj |
steps,of |
R5066 |
T8139 |
T8138 |
amod |
following,steps |
R5067 |
T8140 |
T8138 |
compound |
NaCl,steps |
R5068 |
T8141 |
T8130 |
auxpass |
was,used |
R5069 |
T8142 |
T8130 |
prep |
for,used |
R5070 |
T8143 |
T8144 |
det |
this,assay |
R5071 |
T8144 |
T8142 |
pobj |
assay,for |
R5072 |
T8145 |
T8130 |
punct |
: ,used |
R5073 |
T8146 |
T8147 |
nummod |
0.90,% |
R5074 |
T8147 |
T8130 |
dobj |
%,used |
R5075 |
T8148 |
T8147 |
punct |
", ",% |
R5076 |
T8149 |
T8150 |
nummod |
0.65,% |
R5077 |
T8150 |
T8147 |
appos |
%,% |
R5078 |
T8151 |
T8147 |
punct |
", ",% |
R5079 |
T8152 |
T8153 |
nummod |
0.60,% |
R5080 |
T8153 |
T8147 |
appos |
%,% |
R5081 |
T8154 |
T8147 |
punct |
", ",% |
R5082 |
T8155 |
T8156 |
nummod |
0.55,% |
R5083 |
T8156 |
T8147 |
appos |
%,% |
R5084 |
T8157 |
T8147 |
punct |
", ",% |
R5085 |
T8158 |
T8159 |
nummod |
0.50,% |
R5086 |
T8159 |
T8147 |
appos |
%,% |
R5087 |
T8160 |
T8147 |
punct |
", ",% |
R5088 |
T8161 |
T8162 |
nummod |
0.45,% |
R5089 |
T8162 |
T8147 |
appos |
%,% |
R5090 |
T8163 |
T8147 |
punct |
", ",% |
R5091 |
T8164 |
T8165 |
nummod |
0.40,% |
R5092 |
T8165 |
T8147 |
appos |
%,% |
R5093 |
T8166 |
T8147 |
punct |
", ",% |
R5094 |
T8167 |
T8168 |
nummod |
0.35,% |
R5095 |
T8168 |
T8147 |
appos |
%,% |
R5096 |
T8169 |
T8147 |
punct |
", ",% |
R5097 |
T8170 |
T8171 |
nummod |
0.30,% |
R5098 |
T8171 |
T8147 |
appos |
%,% |
R5099 |
T8172 |
T8147 |
punct |
", ",% |
R5100 |
T8173 |
T8174 |
nummod |
0.10,% |
R5101 |
T8174 |
T8147 |
appos |
%,% |
R5102 |
T8175 |
T8130 |
punct |
.,used |
R5103 |
T8177 |
T8178 |
prep |
For,inverted |
R5104 |
T8179 |
T8180 |
det |
each,step |
R5105 |
T8180 |
T8177 |
pobj |
step,For |
R5106 |
T8181 |
T8182 |
nummod |
5,ml |
R5107 |
T8182 |
T8178 |
nsubjpass |
ml,inverted |
R5108 |
T8183 |
T8182 |
prep |
of,ml |
R5109 |
T8184 |
T8185 |
det |
the,solution |
R5110 |
T8185 |
T8183 |
pobj |
solution,of |
R5111 |
T8186 |
T8185 |
compound |
gradient,solution |
R5112 |
T8187 |
T8185 |
compound |
step,solution |
R5113 |
T8188 |
T8182 |
cc |
and,ml |
R5114 |
T8189 |
T8190 |
nummod |
50,μl |
R5115 |
T8190 |
T8191 |
compound |
μl,sample |
R5116 |
T8191 |
T8182 |
conj |
sample,ml |
R5117 |
T8192 |
T8191 |
compound |
blood,sample |
R5118 |
T8193 |
T8178 |
auxpass |
were,inverted |
R5119 |
T8194 |
T8178 |
advmod |
carefully,inverted |
R5120 |
T8195 |
T8196 |
nummod |
four,times |
R5121 |
T8196 |
T8178 |
npadvmod |
times,inverted |
R5122 |
T8197 |
T8178 |
cc |
and,inverted |
R5123 |
T8198 |
T8178 |
conj |
incubated,inverted |
R5124 |
T8199 |
T8198 |
prep |
for,incubated |
R5125 |
T8200 |
T8201 |
nummod |
30,minutes |
R5126 |
T8201 |
T8199 |
pobj |
minutes,for |
R5127 |
T8202 |
T8198 |
prep |
in,incubated |
R5128 |
T8203 |
T8204 |
det |
the,dark |
R5129 |
T8204 |
T8202 |
pobj |
dark,in |
R5130 |
T8205 |
T8178 |
punct |
.,inverted |
R5131 |
T8207 |
T8208 |
nsubjpass |
Samples,inverted |
R5132 |
T8209 |
T8208 |
auxpass |
were,inverted |
R5133 |
T8210 |
T8208 |
advmod |
carefully,inverted |
R5134 |
T8211 |
T8208 |
advmod |
again,inverted |
R5135 |
T8212 |
T8208 |
cc |
and,inverted |
R5136 |
T8213 |
T8208 |
conj |
centrifuged,inverted |
R5137 |
T8214 |
T8213 |
prep |
at,centrifuged |
R5138 |
T8215 |
T8216 |
nummod |
"1,500",g |
R5139 |
T8216 |
T8214 |
pobj |
g,at |
R5140 |
T8217 |
T8216 |
punct |
×,g |
R5141 |
T8218 |
T8213 |
prep |
for,centrifuged |
R5142 |
T8219 |
T8220 |
nummod |
10,minutes |
R5143 |
T8220 |
T8218 |
pobj |
minutes,for |
R5144 |
T8221 |
T8208 |
punct |
.,inverted |
R5145 |
T8223 |
T8224 |
prep |
From,measured |
R5146 |
T8225 |
T8226 |
nummod |
1,ml |
R5147 |
T8226 |
T8227 |
compound |
ml,supernatant |
R5148 |
T8227 |
T8223 |
pobj |
supernatant,From |
R5149 |
T8228 |
T8227 |
prep |
of,supernatant |
R5150 |
T8229 |
T8230 |
det |
each,step |
R5151 |
T8230 |
T8228 |
pobj |
step,of |
R5152 |
T8231 |
T8232 |
det |
the,extinction |
R5153 |
T8232 |
T8224 |
nsubjpass |
extinction,measured |
R5154 |
T8233 |
T8232 |
prep |
at,extinction |
R5155 |
T8234 |
T8235 |
nummod |
546,nm |
R5156 |
T8235 |
T8236 |
compound |
nm,wavelength |
R5157 |
T8236 |
T8233 |
pobj |
wavelength,at |
R5158 |
T8237 |
T8224 |
auxpass |
was,measured |
R5159 |
T8238 |
T8224 |
punct |
.,measured |
R5160 |
T8240 |
T8241 |
det |
The,experiment |
R5161 |
T8241 |
T8242 |
nsubjpass |
experiment,performed |
R5162 |
T8243 |
T8242 |
auxpass |
was,performed |
R5163 |
T8244 |
T8242 |
prep |
at,performed |
R5164 |
T8245 |
T8246 |
compound |
room,temperature |
R5165 |
T8246 |
T8244 |
pobj |
temperature,at |
R5166 |
T8247 |
T8242 |
punct |
.,performed |
R5167 |
T8326 |
T8327 |
compound |
Platelet,aggregation |
R5168 |
T8327 |
T8328 |
compound |
aggregation,assay |
R5169 |
T8330 |
T8331 |
advmod |
Approximately,500 |
R5170 |
T8331 |
T8332 |
nummod |
500,μl |
R5171 |
T8332 |
T8333 |
nsubjpass |
μl,centrifuged |
R5172 |
T8334 |
T8332 |
prep |
of,μl |
R5173 |
T8335 |
T8336 |
amod |
murine,blood |
R5174 |
T8336 |
T8334 |
pobj |
blood,of |
R5175 |
T8337 |
T8333 |
auxpass |
were,centrifuged |
R5176 |
T8338 |
T8333 |
prep |
at,centrifuged |
R5177 |
T8339 |
T8340 |
nummod |
200,g |
R5178 |
T8340 |
T8338 |
pobj |
g,at |
R5179 |
T8341 |
T8340 |
punct |
×,g |
R5180 |
T8342 |
T8333 |
prep |
for,centrifuged |
R5181 |
T8343 |
T8344 |
nummod |
15,minutes |
R5182 |
T8344 |
T8342 |
pobj |
minutes,for |
R5183 |
T8345 |
T8333 |
punct |
.,centrifuged |
R5184 |
T8347 |
T8348 |
det |
The,supernatants |
R5185 |
T8348 |
T8350 |
nsubjpass |
supernatants,transferred |
R5186 |
T8349 |
T8348 |
amod |
resulting,supernatants |
R5187 |
T8351 |
T8350 |
auxpass |
were,transferred |
R5188 |
T8352 |
T8350 |
prep |
to,transferred |
R5189 |
T8353 |
T8354 |
amod |
new,tubes |
R5190 |
T8354 |
T8352 |
pobj |
tubes,to |
R5191 |
T8355 |
T8350 |
cc |
and,transferred |
R5192 |
T8356 |
T8350 |
conj |
used,transferred |
R5193 |
T8357 |
T8356 |
prep |
as,used |
R5194 |
T8358 |
T8359 |
npadvmod |
platelet,rich |
R5195 |
T8359 |
T8360 |
amod |
rich,plasma |
R5196 |
T8360 |
T8357 |
pobj |
plasma,as |
R5197 |
T8361 |
T8360 |
punct |
(,plasma |
R5198 |
T8362 |
T8360 |
appos |
PRP,plasma |
R5199 |
T8363 |
T8350 |
punct |
),transferred |
R5200 |
T8364 |
T8350 |
punct |
.,transferred |
R5201 |
T8366 |
T8367 |
det |
The,measurements |
R5202 |
T8367 |
T8370 |
nsubjpass |
measurements,done |
R5203 |
T8368 |
T8369 |
compound |
platelet,aggregation |
R5204 |
T8369 |
T8367 |
compound |
aggregation,measurements |
R5205 |
T8371 |
T8370 |
auxpass |
were,done |
R5206 |
T8372 |
T8370 |
prep |
according,done |
R5207 |
T8373 |
T8372 |
prep |
to,according |
R5208 |
T8374 |
T8375 |
det |
the,method |
R5209 |
T8375 |
T8373 |
pobj |
method,to |
R5210 |
T8376 |
T8375 |
acl |
developed,method |
R5211 |
T8377 |
T8376 |
agent |
by,developed |
R5212 |
T8378 |
T8377 |
pobj |
Born,by |
R5213 |
T8379 |
T8378 |
cc |
and,Born |
R5214 |
T8380 |
T8378 |
conj |
Cross,Born |
R5215 |
T8381 |
T8382 |
punct |
[,26 |
R5216 |
T8382 |
T8380 |
parataxis |
26,Cross |
R5217 |
T8383 |
T8382 |
punct |
],26 |
R5218 |
T8384 |
T8370 |
advcl |
using,done |
R5219 |
T8385 |
T8386 |
det |
an,photometer |
R5220 |
T8386 |
T8384 |
dobj |
photometer,using |
R5221 |
T8387 |
T8386 |
compound |
APACT,photometer |
R5222 |
T8388 |
T8389 |
punct |
(,FRG |
R5223 |
T8389 |
T8386 |
parataxis |
FRG,photometer |
R5224 |
T8390 |
T8389 |
dep |
Labor,FRG |
R5225 |
T8391 |
T8389 |
punct |
", ",FRG |
R5226 |
T8392 |
T8389 |
dep |
Ahrendsburg,FRG |
R5227 |
T8393 |
T8389 |
punct |
", ",FRG |
R5228 |
T8394 |
T8389 |
punct |
),FRG |
R5229 |
T8395 |
T8386 |
cc |
and,photometer |
R5230 |
T8396 |
T8397 |
det |
the,software |
R5231 |
T8397 |
T8386 |
conj |
software,photometer |
R5232 |
T8398 |
T8397 |
compound |
APACT,software |
R5233 |
T8399 |
T8400 |
punct |
(,APACT |
R5234 |
T8400 |
T8397 |
parataxis |
APACT,software |
R5235 |
T8401 |
T8400 |
nummod |
1.4,APACT |
R5236 |
T8402 |
T8400 |
punct |
),APACT |
R5237 |
T8403 |
T8370 |
punct |
.,done |
R5238 |
T8405 |
T8406 |
advmod |
Subsequently,adjusted |
R5239 |
T8407 |
T8406 |
nsubjpass |
samples,adjusted |
R5240 |
T8408 |
T8407 |
prep |
of,samples |
R5241 |
T8409 |
T8410 |
det |
the,PRP |
R5242 |
T8410 |
T8408 |
pobj |
PRP,of |
R5243 |
T8411 |
T8406 |
auxpass |
were,adjusted |
R5244 |
T8412 |
T8406 |
prep |
with,adjusted |
R5245 |
T8413 |
T8412 |
pobj |
buffer,with |
R5246 |
T8414 |
T8415 |
punct |
(,pH |
R5247 |
T8415 |
T8413 |
parataxis |
pH,buffer |
R5248 |
T8416 |
T8417 |
nummod |
5,mM |
R5249 |
T8417 |
T8418 |
compound |
mM,Hepes |
R5250 |
T8418 |
T8415 |
dep |
Hepes,pH |
R5251 |
T8419 |
T8415 |
punct |
", ",pH |
R5252 |
T8420 |
T8421 |
nummod |
150,mM |
R5253 |
T8421 |
T8422 |
compound |
mM,NaCl |
R5254 |
T8422 |
T8415 |
dep |
NaCl,pH |
R5255 |
T8423 |
T8415 |
punct |
", ",pH |
R5256 |
T8424 |
T8415 |
nummod |
7.3,pH |
R5257 |
T8425 |
T8415 |
punct |
),pH |
R5258 |
T8426 |
T8406 |
prep |
to,adjusted |
R5259 |
T8427 |
T8428 |
nummod |
"250,000",platelets |
R5260 |
T8428 |
T8426 |
pobj |
platelets,to |
R5261 |
T8429 |
T8430 |
punct |
/,μl |
R5262 |
T8430 |
T8428 |
prep |
μl,platelets |
R5263 |
T8431 |
T8406 |
punct |
.,adjusted |
R5264 |
T8433 |
T8434 |
det |
A,count |
R5265 |
T8434 |
T8437 |
nsubjpass |
count,used |
R5266 |
T8435 |
T8434 |
amod |
constant,count |
R5267 |
T8436 |
T8434 |
compound |
platelet,count |
R5268 |
T8438 |
T8437 |
auxpass |
was,used |
R5269 |
T8439 |
T8437 |
advmod |
throughout,used |
R5270 |
T8440 |
T8437 |
advcl |
allowing,used |
R5271 |
T8441 |
T8440 |
dobj |
comparisons,allowing |
R5272 |
T8442 |
T8441 |
prep |
between,comparisons |
R5273 |
T8443 |
T8444 |
amod |
wild,type |
R5274 |
T8444 |
T8445 |
nmod |
type,samples |
R5275 |
T8445 |
T8442 |
pobj |
samples,between |
R5276 |
T8446 |
T8444 |
cc |
and,type |
R5277 |
T8447 |
T8448 |
compound |
knock,out |
R5278 |
T8448 |
T8444 |
conj |
out,type |
R5279 |
T8449 |
T8445 |
amod |
murine,samples |
R5280 |
T8450 |
T8449 |
cc |
and,murine |
R5281 |
T8451 |
T8449 |
conj |
human,murine |
R5282 |
T8452 |
T8437 |
punct |
.,used |
R5283 |
T8454 |
T8455 |
nummod |
250,μl |
R5284 |
T8455 |
T8456 |
nmod |
μl,platelets |
R5285 |
T8456 |
T8458 |
nsubjpass |
platelets,prewarmed |
R5286 |
T8457 |
T8456 |
amod |
adjusted,platelets |
R5287 |
T8459 |
T8458 |
auxpass |
were,prewarmed |
R5288 |
T8460 |
T8458 |
prep |
to,prewarmed |
R5289 |
T8461 |
T8462 |
nummod |
37,°C |
R5290 |
T8462 |
T8460 |
pobj |
°C,to |
R5291 |
T8463 |
T8458 |
cc |
and,prewarmed |
R5292 |
T8464 |
T8458 |
conj |
used,prewarmed |
R5293 |
T8465 |
T8464 |
prep |
in,used |
R5294 |
T8466 |
T8467 |
det |
the,assay |
R5295 |
T8467 |
T8465 |
pobj |
assay,in |
R5296 |
T8468 |
T8458 |
punct |
", ",prewarmed |
R5297 |
T8469 |
T8458 |
cc |
and,prewarmed |
R5298 |
T8470 |
T8471 |
det |
the,process |
R5299 |
T8471 |
T8473 |
nsubjpass |
process,started |
R5300 |
T8472 |
T8471 |
compound |
aggregation,process |
R5301 |
T8473 |
T8458 |
conj |
started,prewarmed |
R5302 |
T8474 |
T8473 |
auxpass |
was,started |
R5303 |
T8475 |
T8473 |
prep |
by,started |
R5304 |
T8476 |
T8475 |
pcomp |
adding,by |
R5305 |
T8477 |
T8478 |
nummod |
25,μl |
R5306 |
T8478 |
T8476 |
dobj |
μl,adding |
R5307 |
T8479 |
T8478 |
prep |
of,μl |
R5308 |
T8480 |
T8481 |
det |
a,solution |
R5309 |
T8481 |
T8479 |
pobj |
solution,of |
R5310 |
T8482 |
T8481 |
amod |
prewarmed,solution |
R5311 |
T8483 |
T8484 |
nummod |
16.5,mg |
R5312 |
T8484 |
T8481 |
nmod |
mg,solution |
R5313 |
T8485 |
T8486 |
punct |
/,ml |
R5314 |
T8486 |
T8484 |
prep |
ml,mg |
R5315 |
T8487 |
T8481 |
compound |
ristocetin,solution |
R5316 |
T8488 |
T8489 |
punct |
(,FRG |
R5317 |
T8489 |
T8481 |
parataxis |
FRG,solution |
R5318 |
T8490 |
T8491 |
compound |
DiaMed,Diagnostica |
R5319 |
T8491 |
T8489 |
dep |
Diagnostica,FRG |
R5320 |
T8492 |
T8489 |
punct |
", ",FRG |
R5321 |
T8493 |
T8489 |
dep |
Bensheim,FRG |
R5322 |
T8494 |
T8489 |
punct |
", ",FRG |
R5323 |
T8495 |
T8489 |
punct |
;,FRG |
R5324 |
T8496 |
T8497 |
dep |
concentration,mg |
R5325 |
T8497 |
T8489 |
dep |
mg,FRG |
R5326 |
T8498 |
T8496 |
prep |
in,concentration |
R5327 |
T8499 |
T8500 |
det |
the,assay |
R5328 |
T8500 |
T8498 |
pobj |
assay,in |
R5329 |
T8501 |
T8497 |
punct |
: ,mg |
R5330 |
T8502 |
T8497 |
nummod |
1.5,mg |
R5331 |
T8503 |
T8504 |
punct |
/,ml |
R5332 |
T8504 |
T8497 |
prep |
ml,mg |
R5333 |
T8505 |
T8489 |
punct |
),FRG |
R5334 |
T8506 |
T8481 |
punct |
", ",solution |
R5335 |
T8507 |
T8508 |
det |
a,reagent |
R5336 |
T8508 |
T8481 |
appos |
reagent,solution |
R5337 |
T8509 |
T8510 |
dep |
which,worked |
R5338 |
T8510 |
T8508 |
relcl |
worked,reagent |
R5339 |
T8511 |
T8510 |
advmod |
reliably,worked |
R5340 |
T8512 |
T8510 |
prep |
in,worked |
R5341 |
T8513 |
T8514 |
poss |
our,hands |
R5342 |
T8514 |
T8512 |
pobj |
hands,in |
R5343 |
T8515 |
T8510 |
prep |
with,worked |
R5344 |
T8516 |
T8517 |
nmod |
mouse,platelets |
R5345 |
T8517 |
T8515 |
pobj |
platelets,with |
R5346 |
T8518 |
T8516 |
cc |
and,mouse |
R5347 |
T8519 |
T8516 |
conj |
human,mouse |
R5348 |
T8520 |
T8473 |
punct |
.,started |
R5349 |
T8522 |
T8523 |
det |
Each,assay |
R5350 |
T8523 |
T8525 |
nsubj |
assay,represents |
R5351 |
T8524 |
T8523 |
amod |
murine,assay |
R5352 |
T8526 |
T8527 |
det |
a,animal |
R5353 |
T8527 |
T8525 |
dobj |
animal,represents |
R5354 |
T8528 |
T8527 |
amod |
different,animal |
R5355 |
T8529 |
T8525 |
punct |
.,represents |
R5356 |
T8531 |
T8532 |
nsubjpass |
Analyses,performed |
R5357 |
T8533 |
T8531 |
prep |
of,Analyses |
R5358 |
T8534 |
T8535 |
amod |
human,samples |
R5359 |
T8535 |
T8533 |
pobj |
samples,of |
R5360 |
T8536 |
T8535 |
compound |
blood,samples |
R5361 |
T8537 |
T8532 |
auxpass |
were,performed |
R5362 |
T8538 |
T8539 |
mark |
as,described |
R5363 |
T8539 |
T8532 |
advcl |
described,performed |
R5364 |
T8540 |
T8539 |
prep |
in,described |
R5365 |
T8541 |
T8542 |
det |
the,manufacturer |
R5366 |
T8542 |
T8543 |
poss |
manufacturer,protocol |
R5367 |
T8543 |
T8540 |
pobj |
protocol,in |
R5368 |
T8544 |
T8542 |
case |
's,manufacturer |
R5369 |
T8545 |
T8532 |
punct |
.,performed |
R5370 |
T8547 |
T8548 |
amod |
Several,measurements |
R5371 |
T8548 |
T8550 |
nsubjpass |
measurements,done |
R5372 |
T8549 |
T8548 |
amod |
repeated,measurements |
R5373 |
T8551 |
T8550 |
auxpass |
were,done |
R5374 |
T8552 |
T8550 |
prep |
per,done |
R5375 |
T8553 |
T8554 |
amod |
human,individual |
R5376 |
T8554 |
T8552 |
pobj |
individual,per |
R5377 |
T8555 |
T8556 |
aux |
to,control |
R5378 |
T8556 |
T8550 |
advcl |
control,done |
R5379 |
T8557 |
T8558 |
det |
the,reproducibility |
R5380 |
T8558 |
T8556 |
dobj |
reproducibility,control |
R5381 |
T8559 |
T8550 |
punct |
.,done |
R5382 |
T8561 |
T8562 |
det |
The,data |
R5383 |
T8562 |
T8566 |
nsubjpass |
data,analysed |
R5384 |
T8563 |
T8562 |
amod |
acquired,data |
R5385 |
T8564 |
T8565 |
compound |
aggregation,curve |
R5386 |
T8565 |
T8562 |
compound |
curve,data |
R5387 |
T8567 |
T8562 |
punct |
", ",data |
R5388 |
T8568 |
T8562 |
acl |
measured,data |
R5389 |
T8569 |
T8568 |
prep |
as,measured |
R5390 |
T8570 |
T8571 |
amod |
ascending,degree |
R5391 |
T8571 |
T8569 |
pobj |
degree,as |
R5392 |
T8572 |
T8571 |
prep |
of,degree |
R5393 |
T8573 |
T8572 |
pobj |
transmission,of |
R5394 |
T8574 |
T8566 |
punct |
", ",analysed |
R5395 |
T8575 |
T8566 |
auxpass |
were,analysed |
R5396 |
T8576 |
T8566 |
prep |
for,analysed |
R5397 |
T8577 |
T8578 |
amod |
maximal,values |
R5398 |
T8578 |
T8576 |
pobj |
values,for |
R5399 |
T8579 |
T8578 |
compound |
slope,values |
R5400 |
T8580 |
T8566 |
punct |
", ",analysed |
R5401 |
T8581 |
T8582 |
prep |
while,set |
R5402 |
T8582 |
T8566 |
advcl |
set,analysed |
R5403 |
T8583 |
T8584 |
det |
the,amplitude |
R5404 |
T8584 |
T8582 |
nsubjpass |
amplitude,set |
R5405 |
T8585 |
T8584 |
amod |
maximal,amplitude |
R5406 |
T8586 |
T8584 |
compound |
aggregation,amplitude |
R5407 |
T8587 |
T8584 |
prep |
of,amplitude |
R5408 |
T8588 |
T8589 |
det |
every,curve |
R5409 |
T8589 |
T8587 |
pobj |
curve,of |
R5410 |
T8590 |
T8589 |
amod |
single,curve |
R5411 |
T8591 |
T8582 |
auxpass |
was,set |
R5412 |
T8592 |
T8582 |
prep |
to,set |
R5413 |
T8593 |
T8594 |
nummod |
100,% |
R5414 |
T8594 |
T8592 |
pobj |
%,to |
R5415 |
T8595 |
T8566 |
punct |
.,analysed |
R5416 |
T8597 |
T8598 |
prep |
In,done |
R5417 |
T8599 |
T8597 |
amod |
general,In |
R5418 |
T8600 |
T8598 |
punct |
", ",done |
R5419 |
T8601 |
T8602 |
det |
all,procedures |
R5420 |
T8602 |
T8598 |
nsubjpass |
procedures,done |
R5421 |
T8603 |
T8602 |
compound |
platelet,procedures |
R5422 |
T8604 |
T8598 |
auxpass |
were,done |
R5423 |
T8605 |
T8598 |
prep |
at,done |
R5424 |
T8606 |
T8607 |
nummod |
22,°C |
R5425 |
T8607 |
T8605 |
pobj |
°C,at |
R5426 |
T8608 |
T8598 |
prep |
within,done |
R5427 |
T8609 |
T8610 |
nummod |
four,hours |
R5428 |
T8610 |
T8608 |
pobj |
hours,within |
R5429 |
T8611 |
T8610 |
prep |
after,hours |
R5430 |
T8612 |
T8611 |
pcomp |
collecting,after |
R5431 |
T8613 |
T8614 |
det |
the,sample |
R5432 |
T8614 |
T8612 |
dobj |
sample,collecting |
R5433 |
T8615 |
T8614 |
compound |
blood,sample |
R5434 |
T8616 |
T8598 |
punct |
.,done |
R5435 |
T8735 |
T8736 |
amod |
Miscellaneous,methods |
R5436 |
T8738 |
T8739 |
prep |
For,analysed |
R5437 |
T8740 |
T8741 |
amod |
standard,measurements |
R5438 |
T8741 |
T8738 |
pobj |
measurements,For |
R5439 |
T8742 |
T8741 |
amod |
haematopoetic,measurements |
R5440 |
T8743 |
T8741 |
cc |
and,measurements |
R5441 |
T8744 |
T8745 |
compound |
cell,counts |
R5442 |
T8745 |
T8741 |
conj |
counts,measurements |
R5443 |
T8746 |
T8741 |
prep |
on,measurements |
R5444 |
T8747 |
T8748 |
nmod |
murine,samples |
R5445 |
T8748 |
T8746 |
pobj |
samples,on |
R5446 |
T8749 |
T8747 |
cc |
and,murine |
R5447 |
T8750 |
T8747 |
conj |
human,murine |
R5448 |
T8751 |
T8748 |
compound |
blood,samples |
R5449 |
T8752 |
T8753 |
quantmod |
about,300 |
R5450 |
T8753 |
T8754 |
nummod |
300,μl |
R5451 |
T8754 |
T8739 |
nsubjpass |
μl,analysed |
R5452 |
T8755 |
T8754 |
prep |
of,μl |
R5453 |
T8756 |
T8755 |
pobj |
blood,of |
R5454 |
T8757 |
T8739 |
auxpass |
were,analysed |
R5455 |
T8758 |
T8739 |
prep |
with,analysed |
R5456 |
T8759 |
T8760 |
det |
an,counter |
R5457 |
T8760 |
T8758 |
pobj |
counter,with |
R5458 |
T8761 |
T8760 |
nmod |
ADVIA,counter |
R5459 |
T8762 |
T8761 |
nummod |
120,ADVIA |
R5460 |
T8763 |
T8760 |
amod |
electronic,counter |
R5461 |
T8764 |
T8760 |
compound |
cell,counter |
R5462 |
T8765 |
T8766 |
punct |
(,Bayer |
R5463 |
T8766 |
T8760 |
parataxis |
Bayer,counter |
R5464 |
T8767 |
T8766 |
punct |
),Bayer |
R5465 |
T8768 |
T8739 |
punct |
.,analysed |
R5466 |
T8770 |
T8771 |
prep |
With,transformed |
R5467 |
T8772 |
T8773 |
det |
this,equipment |
R5468 |
T8773 |
T8770 |
pobj |
equipment,With |
R5469 |
T8774 |
T8775 |
amod |
red,cells |
R5470 |
T8775 |
T8771 |
nsubjpass |
cells,transformed |
R5471 |
T8776 |
T8775 |
compound |
blood,cells |
R5472 |
T8777 |
T8775 |
cc |
and,cells |
R5473 |
T8778 |
T8775 |
conj |
platelets,cells |
R5474 |
T8779 |
T8771 |
auxpass |
are,transformed |
R5475 |
T8780 |
T8771 |
punct |
"""",transformed |
R5476 |
T8781 |
T8771 |
punct |
"""",transformed |
R5477 |
T8782 |
T8771 |
prep |
into,transformed |
R5478 |
T8783 |
T8784 |
amod |
spherical,bodies |
R5479 |
T8784 |
T8782 |
pobj |
bodies,into |
R5480 |
T8785 |
T8771 |
prep |
without,transformed |
R5481 |
T8786 |
T8785 |
pcomp |
changing,without |
R5482 |
T8787 |
T8788 |
poss |
their,volume |
R5483 |
T8788 |
T8786 |
dobj |
volume,changing |
R5484 |
T8789 |
T8771 |
punct |
.,transformed |
R5485 |
T8791 |
T8792 |
advmod |
Therefore,are |
R5486 |
T8793 |
T8792 |
nsubj |
number,are |
R5487 |
T8794 |
T8793 |
cc |
and,number |
R5488 |
T8795 |
T8793 |
conj |
volume,number |
R5489 |
T8796 |
T8793 |
prep |
of,number |
R5490 |
T8797 |
T8798 |
amod |
red,cells |
R5491 |
T8798 |
T8796 |
pobj |
cells,of |
R5492 |
T8799 |
T8798 |
compound |
blood,cells |
R5493 |
T8800 |
T8798 |
cc |
and,cells |
R5494 |
T8801 |
T8798 |
conj |
platelets,cells |
R5495 |
T8802 |
T8792 |
neg |
not,are |
R5496 |
T8803 |
T8804 |
amod |
calculated,values |
R5497 |
T8804 |
T8792 |
attr |
values,are |
R5498 |
T8805 |
T8792 |
punct |
", ",are |
R5499 |
T8806 |
T8792 |
cc |
but,are |
R5500 |
T8807 |
T8808 |
advmod |
directly,measured |
R5501 |
T8808 |
T8809 |
amod |
measured,values |
R5502 |
T8809 |
T8792 |
conj |
values,are |
R5503 |
T8810 |
T8792 |
punct |
.,are |
R5504 |
T8812 |
T8813 |
det |
The,data |
R5505 |
T8813 |
T8815 |
nsubjpass |
data,analysed |
R5506 |
T8814 |
T8813 |
amod |
following,data |
R5507 |
T8816 |
T8815 |
auxpass |
were,analysed |
R5508 |
T8817 |
T8815 |
advmod |
statistically,analysed |
R5509 |
T8818 |
T8815 |
punct |
: ,analysed |
R5510 |
T8819 |
T8815 |
dobj |
leucocytes,analysed |
R5511 |
T8820 |
T8819 |
punct |
", ",leucocytes |
R5512 |
T8821 |
T8822 |
amod |
red,cells |
R5513 |
T8822 |
T8819 |
appos |
cells,leucocytes |
R5514 |
T8823 |
T8822 |
compound |
blood,cells |
R5515 |
T8824 |
T8819 |
punct |
", ",leucocytes |
R5516 |
T8825 |
T8819 |
appos |
haemoglobin,leucocytes |
R5517 |
T8826 |
T8819 |
punct |
", ",leucocytes |
R5518 |
T8827 |
T8819 |
appos |
haematocrit,leucocytes |
R5519 |
T8828 |
T8819 |
punct |
", ",leucocytes |
R5520 |
T8829 |
T8830 |
amod |
mean,volume |
R5521 |
T8830 |
T8819 |
appos |
volume,leucocytes |
R5522 |
T8831 |
T8830 |
amod |
cellular,volume |
R5523 |
T8832 |
T8819 |
punct |
", ",leucocytes |
R5524 |
T8833 |
T8834 |
amod |
mean,haemoglobin |
R5525 |
T8834 |
T8819 |
appos |
haemoglobin,leucocytes |
R5526 |
T8835 |
T8834 |
amod |
cellular,haemoglobin |
R5527 |
T8836 |
T8819 |
punct |
", ",leucocytes |
R5528 |
T8837 |
T8838 |
amod |
mean,haemoglobin |
R5529 |
T8838 |
T8840 |
compound |
haemoglobin,concentration |
R5530 |
T8839 |
T8838 |
amod |
cellular,haemoglobin |
R5531 |
T8840 |
T8819 |
appos |
concentration,leucocytes |
R5532 |
T8841 |
T8819 |
punct |
", ",leucocytes |
R5533 |
T8842 |
T8843 |
compound |
platelet,number |
R5534 |
T8843 |
T8819 |
appos |
number,leucocytes |
R5535 |
T8844 |
T8819 |
punct |
", ",leucocytes |
R5536 |
T8845 |
T8819 |
appos |
neutrophiles,leucocytes |
R5537 |
T8846 |
T8819 |
punct |
", ",leucocytes |
R5538 |
T8847 |
T8819 |
appos |
lymphocytes,leucocytes |
R5539 |
T8848 |
T8819 |
punct |
", ",leucocytes |
R5540 |
T8849 |
T8819 |
appos |
monocytes,leucocytes |
R5541 |
T8850 |
T8819 |
punct |
", ",leucocytes |
R5542 |
T8851 |
T8819 |
appos |
eosinophiles,leucocytes |
R5543 |
T8852 |
T8819 |
punct |
", ",leucocytes |
R5544 |
T8853 |
T8819 |
appos |
basophiles,leucocytes |
R5545 |
T8854 |
T8815 |
punct |
.,analysed |
R5546 |
T8856 |
T8857 |
compound |
Peptide,mass |
R5547 |
T8857 |
T8858 |
compound |
mass,fingerprinting |
R5548 |
T8858 |
T8859 |
nsubjpass |
fingerprinting,performed |
R5549 |
T8860 |
T8859 |
auxpass |
was,performed |
R5550 |
T8861 |
T8859 |
prep |
on,performed |
R5551 |
T8862 |
T8863 |
det |
a,spectrometer |
R5552 |
T8863 |
T8861 |
pobj |
spectrometer,on |
R5553 |
T8864 |
T8865 |
nmod |
Bruker,Reflex |
R5554 |
T8865 |
T8863 |
nmod |
Reflex,spectrometer |
R5555 |
T8866 |
T8865 |
nummod |
IV,Reflex |
R5556 |
T8867 |
T8868 |
compound |
MALDI,TOF |
R5557 |
T8868 |
T8863 |
compound |
TOF,spectrometer |
R5558 |
T8869 |
T8868 |
punct |
-,TOF |
R5559 |
T8870 |
T8863 |
compound |
mass,spectrometer |
R5560 |
T8871 |
T8859 |
punct |
.,performed |
R5561 |
T8873 |
T8874 |
amod |
Recombinant,A7 |
R5562 |
T8874 |
T8876 |
nsubjpass |
A7,purified |
R5563 |
T8875 |
T8874 |
compound |
annexin,A7 |
R5564 |
T8876 |
T8878 |
ccomp |
purified,done |
R5565 |
T8877 |
T8876 |
auxpass |
was,purified |
R5566 |
T8879 |
T8876 |
prep |
according,purified |
R5567 |
T8880 |
T8879 |
prep |
to,according |
R5568 |
T8881 |
T8880 |
punct |
[,to |
R5569 |
T8882 |
T8880 |
pobj |
27,to |
R5570 |
T8883 |
T8878 |
punct |
],done |
R5571 |
T8884 |
T8878 |
punct |
", ",done |
R5572 |
T8885 |
T8886 |
compound |
F,actin |
R5573 |
T8886 |
T8878 |
nsubjpass |
actin,done |
R5574 |
T8887 |
T8886 |
punct |
-,actin |
R5575 |
T8888 |
T8886 |
punct |
-,actin |
R5576 |
T8889 |
T8886 |
amod |
binding,actin |
R5577 |
T8890 |
T8886 |
prep |
of,actin |
R5578 |
T8891 |
T8892 |
amod |
recombinant,A7 |
R5579 |
T8892 |
T8890 |
pobj |
A7,of |
R5580 |
T8893 |
T8892 |
compound |
annexin,A7 |
R5581 |
T8894 |
T8878 |
auxpass |
was,done |
R5582 |
T8895 |
T8896 |
mark |
as,described |
R5583 |
T8896 |
T8878 |
advcl |
described,done |
R5584 |
T8897 |
T8898 |
punct |
[,28 |
R5585 |
T8898 |
T8878 |
parataxis |
28,done |
R5586 |
T8899 |
T8898 |
punct |
],28 |
R5587 |
T8900 |
T8878 |
punct |
.,done |
R5588 |
T8902 |
T8903 |
compound |
SDS,PAGE |
R5589 |
T8903 |
T8905 |
nsubjpass |
PAGE,done |
R5590 |
T8904 |
T8903 |
punct |
-,PAGE |
R5591 |
T8906 |
T8903 |
cc |
and,PAGE |
R5592 |
T8907 |
T8908 |
amod |
western,blotting |
R5593 |
T8908 |
T8903 |
conj |
blotting,PAGE |
R5594 |
T8909 |
T8905 |
auxpass |
were,done |
R5595 |
T8910 |
T8911 |
mark |
as,described |
R5596 |
T8911 |
T8905 |
advcl |
described,done |
R5597 |
T8912 |
T8913 |
punct |
[,30 |
R5598 |
T8913 |
T8905 |
parataxis |
30,done |
R5599 |
T8914 |
T8913 |
nummod |
29,30 |
R5600 |
T8915 |
T8913 |
punct |
",",30 |
R5601 |
T8916 |
T8913 |
punct |
],30 |
R5602 |
T8917 |
T8905 |
punct |
.,done |
R5603 |
T8919 |
T8920 |
nsubj |
Detection,was |
R5604 |
T8921 |
T8919 |
prep |
in,Detection |
R5605 |
T8922 |
T8921 |
pobj |
immunoblots,in |
R5606 |
T8923 |
T8920 |
prep |
with,was |
R5607 |
T8924 |
T8925 |
amod |
enhanced,chemiluminescence |
R5608 |
T8925 |
T8923 |
pobj |
chemiluminescence,with |
R5609 |
T8926 |
T8927 |
punct |
[,17 |
R5610 |
T8927 |
T8920 |
parataxis |
17,was |
R5611 |
T8928 |
T8927 |
punct |
],17 |
R5612 |
T8929 |
T8920 |
punct |
.,was |
R5613 |
T8931 |
T8932 |
nsubj |
Antibodies,were |
R5614 |
T8933 |
T8931 |
acl |
employed,Antibodies |
R5615 |
T8934 |
T8935 |
nmod |
mab,203 |
R5616 |
T8935 |
T8932 |
attr |
203,were |
R5617 |
T8936 |
T8937 |
punct |
–,217 |
R5618 |
T8937 |
T8935 |
prep |
217,203 |
R5619 |
T8938 |
T8935 |
acl |
directed,203 |
R5620 |
T8939 |
T8938 |
prep |
against,directed |
R5621 |
T8940 |
T8941 |
compound |
mouse,domain |
R5622 |
T8941 |
T8939 |
pobj |
domain,against |
R5623 |
T8942 |
T8943 |
compound |
annexin,A7 |
R5624 |
T8943 |
T8941 |
compound |
A7,domain |
R5625 |
T8944 |
T8941 |
compound |
core,domain |
R5626 |
T8945 |
T8946 |
punct |
[,5 |
R5627 |
T8946 |
T8941 |
parataxis |
5,domain |
R5628 |
T8947 |
T8946 |
punct |
],5 |
R5629 |
T8948 |
T8941 |
cc |
and,domain |
R5630 |
T8949 |
T8950 |
det |
a,antibody |
R5631 |
T8950 |
T8941 |
conj |
antibody,domain |
R5632 |
T8951 |
T8950 |
amod |
polyclonal,antibody |
R5633 |
T8952 |
T8950 |
nmod |
rabbit,antibody |
R5634 |
T8953 |
T8950 |
amod |
anti-sorcin,antibody |
R5635 |
T8954 |
T8955 |
punct |
[,31 |
R5636 |
T8955 |
T8950 |
parataxis |
31,antibody |
R5637 |
T8956 |
T8955 |
punct |
],31 |
R5638 |
T8957 |
T8932 |
punct |
.,were |
R5639 |
T8959 |
T8960 |
prep |
For,performed |
R5640 |
T8961 |
T8962 |
amod |
statistical,analyses |
R5641 |
T8962 |
T8959 |
pobj |
analyses,For |
R5642 |
T8963 |
T8964 |
det |
the,tests |
R5643 |
T8964 |
T8960 |
nsubjpass |
tests,performed |
R5644 |
T8965 |
T8964 |
amod |
following,tests |
R5645 |
T8966 |
T8960 |
auxpass |
were,performed |
R5646 |
T8967 |
T8960 |
punct |
: ,performed |
R5647 |
T8968 |
T8969 |
nmod |
David,test |
R5648 |
T8969 |
T8960 |
dobj |
test,performed |
R5649 |
T8970 |
T8971 |
advmod |
et,al. |
R5650 |
T8971 |
T8968 |
advmod |
al.,David |
R5651 |
T8972 |
T8969 |
compound |
range,test |
R5652 |
T8973 |
T8974 |
aux |
to,ensure |
R5653 |
T8974 |
T8969 |
advcl |
ensure,test |
R5654 |
T8975 |
T8976 |
nsubj |
samples,are |
R5655 |
T8976 |
T8974 |
advcl |
are,ensure |
R5656 |
T8977 |
T8976 |
prep |
from,are |
R5657 |
T8978 |
T8979 |
det |
a,distribution |
R5658 |
T8979 |
T8977 |
pobj |
distribution,from |
R5659 |
T8980 |
T8979 |
amod |
normal,distribution |
R5660 |
T8981 |
T8969 |
punct |
", ",test |
R5661 |
T8982 |
T8983 |
compound |
F,test |
R5662 |
T8983 |
T8969 |
appos |
test,test |
R5663 |
T8984 |
T8983 |
punct |
-,test |
R5664 |
T8985 |
T8983 |
acl |
looking,test |
R5665 |
T8986 |
T8985 |
prep |
for,looking |
R5666 |
T8987 |
T8988 |
det |
a,samples |
R5667 |
T8988 |
T8989 |
poss |
samples,homoscedascity |
R5668 |
T8989 |
T8986 |
pobj |
homoscedascity,for |
R5669 |
T8990 |
T8988 |
case |
',samples |
R5670 |
T8991 |
T8969 |
punct |
", ",test |
R5671 |
T8992 |
T8993 |
poss |
Student,test |
R5672 |
T8993 |
T8969 |
appos |
test,test |
R5673 |
T8994 |
T8992 |
case |
's,Student |
R5674 |
T8995 |
T8993 |
compound |
T,test |
R5675 |
T8996 |
T8993 |
punct |
-,test |
R5676 |
T8997 |
T8998 |
aux |
to,judge |
R5677 |
T8998 |
T8993 |
advcl |
judge,test |
R5678 |
T8999 |
T9000 |
mark |
if,have |
R5679 |
T9000 |
T8998 |
ccomp |
have,judge |
R5680 |
T9001 |
T9000 |
nsubj |
samples,have |
R5681 |
T9002 |
T9001 |
prep |
of,samples |
R5682 |
T9003 |
T9004 |
amod |
different,groups |
R5683 |
T9004 |
T9002 |
pobj |
groups,of |
R5684 |
T9005 |
T9006 |
det |
a,value |
R5685 |
T9006 |
T9000 |
dobj |
value,have |
R5686 |
T9007 |
T9006 |
amod |
different,value |
R5687 |
T9008 |
T9006 |
amod |
mean,value |
R5688 |
T9009 |
T9000 |
cc |
and,have |
R5689 |
T9010 |
T9000 |
conj |
are,have |
R5690 |
T9011 |
T9010 |
attr |
parts,are |
R5691 |
T9012 |
T9010 |
prep |
from,are |
R5692 |
T9013 |
T9014 |
amod |
different,distributions |
R5693 |
T9014 |
T9012 |
pobj |
distributions,from |
R5694 |
T9015 |
T9014 |
amod |
normal,distributions |
R5695 |
T9016 |
T8960 |
punct |
.,performed |
R5696 |
T9018 |
T9019 |
det |
The,values |
R5697 |
T9019 |
T9022 |
nsubjpass |
values,indicated |
R5698 |
T9020 |
T9019 |
amod |
exact,values |
R5699 |
T9021 |
T9019 |
compound |
probability,values |
R5700 |
T9023 |
T9019 |
cc |
and,values |
R5701 |
T9024 |
T9025 |
det |
the,significance |
R5702 |
T9025 |
T9019 |
conj |
significance,values |
R5703 |
T9026 |
T9025 |
prep |
of,significance |
R5704 |
T9027 |
T9028 |
det |
an,analysis |
R5705 |
T9028 |
T9026 |
pobj |
analysis,of |
R5706 |
T9029 |
T9022 |
auxpass |
are,indicated |
R5707 |
T9030 |
T9031 |
advmod |
when,described |
R5708 |
T9031 |
T9022 |
advcl |
described,indicated |
R5709 |
T9032 |
T9033 |
det |
the,experiments |
R5710 |
T9033 |
T9031 |
nsubjpass |
experiments,described |
R5711 |
T9034 |
T9031 |
auxpass |
are,described |
R5712 |
T9035 |
T9022 |
punct |
.,indicated |
R5713 |
T9090 |
T9089 |
prep |
of,Expression |
R5714 |
T9091 |
T9092 |
compound |
annexin,A7 |
R5715 |
T9092 |
T9090 |
pobj |
A7,of |
R5716 |
T9093 |
T9089 |
prep |
in,Expression |
R5717 |
T9094 |
T9095 |
amod |
human,cells |
R5718 |
T9095 |
T9093 |
pobj |
cells,in |
R5719 |
T9096 |
T9095 |
amod |
red,cells |
R5720 |
T9097 |
T9095 |
compound |
blood,cells |
R5721 |
T9098 |
T9099 |
punct |
(,1 |
R5722 |
T9099 |
T9095 |
parataxis |
1,cells |
R5723 |
T9100 |
T9099 |
punct |
),1 |
R5724 |
T9101 |
T9095 |
cc |
and,cells |
R5725 |
T9102 |
T9103 |
amod |
human,platelets |
R5726 |
T9103 |
T9095 |
conj |
platelets,cells |
R5727 |
T9104 |
T9105 |
punct |
(,2 |
R5728 |
T9105 |
T9103 |
parataxis |
2,platelets |
R5729 |
T9106 |
T9105 |
punct |
),2 |
R5730 |
T9107 |
T9089 |
punct |
.,Expression |
R5731 |
T9109 |
T9110 |
compound |
Protein,homogenates |
R5732 |
T9110 |
T9111 |
nsubjpass |
homogenates,separated |
R5733 |
T9112 |
T9111 |
auxpass |
were,separated |
R5734 |
T9113 |
T9111 |
prep |
by,separated |
R5735 |
T9114 |
T9115 |
compound |
SDS,PAGE |
R5736 |
T9115 |
T9113 |
pobj |
PAGE,by |
R5737 |
T9116 |
T9115 |
punct |
-,PAGE |
R5738 |
T9117 |
T9118 |
punct |
(,acrylamide |
R5739 |
T9118 |
T9111 |
parataxis |
acrylamide,separated |
R5740 |
T9119 |
T9120 |
nummod |
12,% |
R5741 |
T9120 |
T9118 |
compound |
%,acrylamide |
R5742 |
T9121 |
T9118 |
punct |
),acrylamide |
R5743 |
T9122 |
T9111 |
punct |
.,separated |
R5744 |
T9124 |
T9125 |
det |
The,blot |
R5745 |
T9125 |
T9128 |
nsubjpass |
blot,probed |
R5746 |
T9126 |
T9125 |
amod |
resulting,blot |
R5747 |
T9127 |
T9125 |
amod |
western,blot |
R5748 |
T9128 |
T9130 |
ccomp |
probed,was |
R5749 |
T9129 |
T9128 |
auxpass |
was,probed |
R5750 |
T9131 |
T9128 |
prep |
with,probed |
R5751 |
T9132 |
T9131 |
pobj |
mAb,with |
R5752 |
T9133 |
T9132 |
nummod |
203–217,mAb |
R5753 |
T9134 |
T9130 |
punct |
", ",was |
R5754 |
T9135 |
T9130 |
nsubj |
visualization,was |
R5755 |
T9136 |
T9130 |
prep |
by,was |
R5756 |
T9137 |
T9138 |
det |
a,antibody |
R5757 |
T9138 |
T9136 |
pobj |
antibody,by |
R5758 |
T9139 |
T9138 |
amod |
secondary,antibody |
R5759 |
T9140 |
T9141 |
npadvmod |
peroxidase,coupled |
R5760 |
T9141 |
T9138 |
amod |
coupled,antibody |
R5761 |
T9142 |
T9138 |
acl |
followed,antibody |
R5762 |
T9143 |
T9142 |
agent |
by,followed |
R5763 |
T9144 |
T9143 |
pobj |
ECL,by |
R5764 |
T9145 |
T9130 |
punct |
.,was |
R5765 |
T9147 |
T9148 |
det |
Both,isoforms |
R5766 |
T9148 |
T9149 |
nsubjpass |
isoforms,detected |
R5767 |
T9149 |
T9151 |
ccomp |
detected,is |
R5768 |
T9150 |
T9149 |
auxpass |
are,detected |
R5769 |
T9152 |
T9149 |
prep |
in,detected |
R5770 |
T9153 |
T9154 |
amod |
red,cells |
R5771 |
T9154 |
T9152 |
pobj |
cells,in |
R5772 |
T9155 |
T9154 |
compound |
blood,cells |
R5773 |
T9156 |
T9151 |
punct |
", ",is |
R5774 |
T9157 |
T9151 |
prep |
in,is |
R5775 |
T9158 |
T9157 |
pobj |
platelets,in |
R5776 |
T9159 |
T9160 |
advmod |
only,isoform |
R5777 |
T9160 |
T9151 |
nsubj |
isoform,is |
R5778 |
T9161 |
T9160 |
det |
the,isoform |
R5779 |
T9162 |
T9160 |
amod |
small,isoform |
R5780 |
T9163 |
T9151 |
acomp |
present,is |
R5781 |
T9164 |
T9151 |
punct |
.,is |
R5782 |
T9241 |
T9240 |
prep |
of,Enrichment |
R5783 |
T9242 |
T9243 |
compound |
annexin,A7 |
R5784 |
T9243 |
T9241 |
pobj |
A7,of |
R5785 |
T9244 |
T9243 |
cc |
and,A7 |
R5786 |
T9245 |
T9243 |
conj |
sorcin,A7 |
R5787 |
T9246 |
T9240 |
prep |
in,Enrichment |
R5788 |
T9247 |
T9246 |
pobj |
exovesicles,in |
R5789 |
T9248 |
T9247 |
acl |
derived,exovesicles |
R5790 |
T9249 |
T9248 |
prep |
from,derived |
R5791 |
T9250 |
T9251 |
amod |
red,cells |
R5792 |
T9251 |
T9249 |
pobj |
cells,from |
R5793 |
T9252 |
T9251 |
compound |
blood,cells |
R5794 |
T9253 |
T9240 |
punct |
.,Enrichment |
R5795 |
T9255 |
T9256 |
nsubjpass |
Vesicles,generated |
R5796 |
T9257 |
T9255 |
prep |
from,Vesicles |
R5797 |
T9258 |
T9259 |
amod |
wild,type |
R5798 |
T9259 |
T9257 |
pobj |
type,from |
R5799 |
T9260 |
T9259 |
punct |
(,type |
R5800 |
T9261 |
T9259 |
appos |
wt,type |
R5801 |
T9262 |
T9259 |
punct |
),type |
R5802 |
T9263 |
T9259 |
cc |
and,type |
R5803 |
T9264 |
T9265 |
nmod |
anxA7,mutant |
R5804 |
T9265 |
T9259 |
conj |
mutant,type |
R5805 |
T9266 |
T9264 |
punct |
-,anxA7 |
R5806 |
T9267 |
T9264 |
punct |
/,anxA7 |
R5807 |
T9268 |
T9264 |
punct |
-,anxA7 |
R5808 |
T9269 |
T9265 |
punct |
(,mutant |
R5809 |
T9270 |
T9265 |
appos |
ko,mutant |
R5810 |
T9271 |
T9256 |
punct |
),generated |
R5811 |
T9272 |
T9256 |
auxpass |
were,generated |
R5812 |
T9273 |
T9256 |
prep |
with,generated |
R5813 |
T9274 |
T9275 |
compound |
Ca2+,ionophore |
R5814 |
T9275 |
T9277 |
compound |
ionophore,treatment |
R5815 |
T9276 |
T9275 |
punct |
/,ionophore |
R5816 |
T9277 |
T9273 |
pobj |
treatment,with |
R5817 |
T9278 |
T9256 |
punct |
", ",generated |
R5818 |
T9279 |
T9256 |
conj |
isolated,generated |
R5819 |
T9280 |
T9279 |
prep |
by,isolated |
R5820 |
T9281 |
T9282 |
amod |
differential,centrifugation |
R5821 |
T9282 |
T9280 |
pobj |
centrifugation,by |
R5822 |
T9283 |
T9279 |
cc |
and,isolated |
R5823 |
T9284 |
T9279 |
conj |
analysed,isolated |
R5824 |
T9285 |
T9284 |
prep |
by,analysed |
R5825 |
T9286 |
T9285 |
pobj |
immunoblotting,by |
R5826 |
T9287 |
T9284 |
prep |
with,analysed |
R5827 |
T9288 |
T9289 |
nmod |
mAb,203 |
R5828 |
T9289 |
T9287 |
pobj |
203,with |
R5829 |
T9290 |
T9291 |
punct |
–,217 |
R5830 |
T9291 |
T9289 |
prep |
217,203 |
R5831 |
T9292 |
T9289 |
cc |
and,203 |
R5832 |
T9293 |
T9294 |
det |
a,antibody |
R5833 |
T9294 |
T9289 |
conj |
antibody,203 |
R5834 |
T9295 |
T9294 |
nmod |
sorcin,antibody |
R5835 |
T9296 |
T9294 |
amod |
polyclonal,antibody |
R5836 |
T9297 |
T9256 |
punct |
.,generated |
R5837 |
T9299 |
T9300 |
prep |
In,are |
R5838 |
T9301 |
T9299 |
amod |
general,In |
R5839 |
T9302 |
T9300 |
punct |
", ",are |
R5840 |
T9303 |
T9304 |
compound |
annexin,A7 |
R5841 |
T9304 |
T9300 |
nsubj |
A7,are |
R5842 |
T9305 |
T9304 |
cc |
and,A7 |
R5843 |
T9306 |
T9304 |
conj |
sorcin,A7 |
R5844 |
T9307 |
T9308 |
advmod |
more,abundant |
R5845 |
T9308 |
T9300 |
acomp |
abundant,are |
R5846 |
T9309 |
T9300 |
prep |
in,are |
R5847 |
T9310 |
T9309 |
pobj |
nanovesicles,in |
R5848 |
T9311 |
T9300 |
punct |
.,are |
R5849 |
T9313 |
T9314 |
det |
The,isoform |
R5850 |
T9314 |
T9317 |
nsubjpass |
isoform,observed |
R5851 |
T9315 |
T9316 |
nummod |
51,kDa |
R5852 |
T9316 |
T9314 |
compound |
kDa,isoform |
R5853 |
T9318 |
T9314 |
prep |
of,isoform |
R5854 |
T9319 |
T9320 |
compound |
annexin,A7 |
R5855 |
T9320 |
T9318 |
pobj |
A7,of |
R5856 |
T9321 |
T9322 |
punct |
(,arrows |
R5857 |
T9322 |
T9314 |
parataxis |
arrows,isoform |
R5858 |
T9323 |
T9322 |
punct |
),arrows |
R5859 |
T9324 |
T9317 |
auxpass |
is,observed |
R5860 |
T9325 |
T9317 |
advmod |
only,observed |
R5861 |
T9326 |
T9317 |
prep |
in,observed |
R5862 |
T9327 |
T9326 |
pobj |
nanovesicles,in |
R5863 |
T9328 |
T9317 |
punct |
.,observed |
R5864 |
T9330 |
T9331 |
nsubjpass |
Samples,normalized |
R5865 |
T9332 |
T9330 |
prep |
of,Samples |
R5866 |
T9333 |
T9334 |
det |
both,types |
R5867 |
T9334 |
T9332 |
pobj |
types,of |
R5868 |
T9335 |
T9334 |
compound |
vesicles,types |
R5869 |
T9336 |
T9331 |
auxpass |
were,normalized |
R5870 |
T9337 |
T9331 |
prep |
according,normalized |
R5871 |
T9338 |
T9337 |
prep |
to,according |
R5872 |
T9339 |
T9340 |
poss |
their,activity |
R5873 |
T9340 |
T9338 |
pobj |
activity,to |
R5874 |
T9341 |
T9342 |
compound |
acetylcholine,esterase |
R5875 |
T9342 |
T9340 |
compound |
esterase,activity |
R5876 |
T9343 |
T9331 |
punct |
.,normalized |
R5877 |
T9345 |
T9346 |
amod |
Human,cells |
R5878 |
T9346 |
T9349 |
nsubjpass |
cells,used |
R5879 |
T9347 |
T9346 |
amod |
red,cells |
R5880 |
T9348 |
T9346 |
compound |
blood,cells |
R5881 |
T9350 |
T9351 |
punct |
(,co |
R5882 |
T9351 |
T9346 |
parataxis |
co,cells |
R5883 |
T9352 |
T9351 |
punct |
),co |
R5884 |
T9353 |
T9349 |
auxpass |
were,used |
R5885 |
T9354 |
T9349 |
prep |
for,used |
R5886 |
T9355 |
T9354 |
pobj |
control,for |
R5887 |
T9356 |
T9349 |
cc |
and,used |
R5888 |
T9357 |
T9349 |
conj |
normalized,used |
R5889 |
T9358 |
T9357 |
advmod |
independently,normalized |
R5890 |
T9359 |
T9349 |
punct |
.,used |
R5891 |
T9398 |
T9397 |
prep |
of,Association |
R5892 |
T9399 |
T9400 |
compound |
annexin,A7 |
R5893 |
T9400 |
T9398 |
pobj |
A7,of |
R5894 |
T9401 |
T9400 |
cc |
and,A7 |
R5895 |
T9402 |
T9400 |
conj |
sorcin,A7 |
R5896 |
T9403 |
T9397 |
prep |
with,Association |
R5897 |
T9404 |
T9405 |
amod |
distinct,fractions |
R5898 |
T9405 |
T9403 |
pobj |
fractions,with |
R5899 |
T9406 |
T9407 |
amod |
red,cell |
R5900 |
T9407 |
T9405 |
compound |
cell,fractions |
R5901 |
T9408 |
T9407 |
compound |
blood,cell |
R5902 |
T9409 |
T9410 |
compound |
plasma,membrane |
R5903 |
T9410 |
T9405 |
compound |
membrane,fractions |
R5904 |
T9411 |
T9397 |
punct |
.,Association |
R5905 |
T9413 |
T9414 |
amod |
Lysed,cells |
R5906 |
T9414 |
T9418 |
nsubjpass |
cells,added |
R5907 |
T9415 |
T9414 |
amod |
human,cells |
R5908 |
T9416 |
T9414 |
amod |
red,cells |
R5909 |
T9417 |
T9414 |
compound |
blood,cells |
R5910 |
T9419 |
T9418 |
auxpass |
were,added |
R5911 |
T9420 |
T9418 |
prep |
to,added |
R5912 |
T9421 |
T9422 |
det |
a,gradient |
R5913 |
T9422 |
T9420 |
pobj |
gradient,to |
R5914 |
T9423 |
T9424 |
npadvmod |
self,forming |
R5915 |
T9424 |
T9422 |
amod |
forming,gradient |
R5916 |
T9425 |
T9422 |
compound |
iodixanol,gradient |
R5917 |
T9426 |
T9422 |
compound |
density,gradient |
R5918 |
T9427 |
T9418 |
punct |
.,added |
R5919 |
T9429 |
T9430 |
det |
The,density |
R5920 |
T9430 |
T9431 |
nsubj |
density,increases |
R5921 |
T9432 |
T9430 |
prep |
of,density |
R5922 |
T9433 |
T9434 |
det |
the,gradient |
R5923 |
T9434 |
T9432 |
pobj |
gradient,of |
R5924 |
T9435 |
T9431 |
prep |
from,increases |
R5925 |
T9436 |
T9435 |
pobj |
left,from |
R5926 |
T9437 |
T9438 |
punct |
(,g |
R5927 |
T9438 |
T9436 |
parataxis |
g,left |
R5928 |
T9439 |
T9438 |
nummod |
1.06,g |
R5929 |
T9440 |
T9441 |
punct |
/,ml |
R5930 |
T9441 |
T9438 |
prep |
ml,g |
R5931 |
T9442 |
T9438 |
punct |
),g |
R5932 |
T9443 |
T9435 |
prep |
to,from |
R5933 |
T9444 |
T9443 |
pobj |
right,to |
R5934 |
T9445 |
T9446 |
punct |
(,g |
R5935 |
T9446 |
T9444 |
parataxis |
g,right |
R5936 |
T9447 |
T9446 |
nummod |
1.20,g |
R5937 |
T9448 |
T9449 |
punct |
/,ml |
R5938 |
T9449 |
T9446 |
prep |
ml,g |
R5939 |
T9450 |
T9446 |
punct |
),g |
R5940 |
T9451 |
T9431 |
punct |
.,increases |
R5941 |
T9453 |
T9454 |
nsubjpass |
Fractions,analysed |
R5942 |
T9455 |
T9454 |
auxpass |
were,analysed |
R5943 |
T9456 |
T9454 |
prep |
by,analysed |
R5944 |
T9457 |
T9456 |
pobj |
immunoblotting,by |
R5945 |
T9458 |
T9454 |
advcl |
using,analysed |
R5946 |
T9459 |
T9460 |
nmod |
mAb,203 |
R5947 |
T9460 |
T9458 |
dobj |
203,using |
R5948 |
T9461 |
T9462 |
punct |
–,217 |
R5949 |
T9462 |
T9460 |
prep |
217,203 |
R5950 |
T9463 |
T9460 |
cc |
and,203 |
R5951 |
T9464 |
T9465 |
det |
the,antibody |
R5952 |
T9465 |
T9460 |
conj |
antibody,203 |
R5953 |
T9466 |
T9465 |
nmod |
sorcin,antibody |
R5954 |
T9467 |
T9465 |
amod |
polyclonal,antibody |
R5955 |
T9468 |
T9454 |
punct |
.,analysed |
R5959 |
T9493 |
T9494 |
amod |
red,cells |
R5960 |
T9494 |
T9492 |
pobj |
cells,of |
R5961 |
T9495 |
T9494 |
compound |
blood,cells |
R5962 |
T9496 |
T9494 |
prep |
from,cells |
R5963 |
T9497 |
T9498 |
nmod |
anxA7,mutant |
R5964 |
T9498 |
T9502 |
nmod |
mutant,mice |
R5965 |
T9499 |
T9497 |
punct |
-,anxA7 |
R5966 |
T9500 |
T9497 |
punct |
/,anxA7 |
R5967 |
T9501 |
T9497 |
punct |
-,anxA7 |
R5968 |
T9502 |
T9496 |
pobj |
mice,from |
R5969 |
T9503 |
T9504 |
punct |
(,B |
R5970 |
T9504 |
T9498 |
parataxis |
B,mutant |
R5971 |
T9505 |
T9504 |
punct |
),B |
R5972 |
T9506 |
T9498 |
cc |
and,mutant |
R5973 |
T9507 |
T9508 |
amod |
wild,type |
R5974 |
T9508 |
T9498 |
conj |
type,mutant |
R5975 |
T9509 |
T9510 |
punct |
(,A |
R5976 |
T9510 |
T9502 |
parataxis |
A,mice |
R5977 |
T9511 |
T9510 |
punct |
),A |
R5978 |
T9512 |
T9491 |
punct |
.,microscopy |
R5979 |
T9514 |
T9515 |
det |
The,diameters |
R5980 |
T9515 |
T9518 |
nsubj |
diameters,are |
R5981 |
T9516 |
T9515 |
amod |
mean,diameters |
R5982 |
T9517 |
T9515 |
amod |
cellular,diameters |
R5983 |
T9519 |
T9520 |
nummod |
6.0,μm |
R5984 |
T9520 |
T9518 |
attr |
μm,are |
R5985 |
T9521 |
T9520 |
cc |
and,μm |
R5986 |
T9522 |
T9523 |
nummod |
5.7,μm |
R5987 |
T9523 |
T9520 |
conj |
μm,μm |
R5988 |
T9524 |
T9518 |
punct |
", ",are |
R5989 |
T9525 |
T9518 |
advmod |
respectively,are |
R5992 |
T9529 |
T9531 |
nsubj |
differences,are |
R5994 |
T9532 |
T9533 |
advmod |
statistically,significant |
R5995 |
T9533 |
T9531 |
acomp |
significant,are |
R5996 |
T9534 |
T9535 |
punct |
(,40 |
R5997 |
T9535 |
T9531 |
parataxis |
40,are |
R5998 |
T9536 |
T9537 |
nsubj |
p,0.015 |
R5999 |
T9537 |
T9535 |
ccomp |
0.015,40 |
R6000 |
T9538 |
T9537 |
punct |
=,0.015 |
R6001 |
T9539 |
T9535 |
punct |
;,40 |
R6002 |
T9540 |
T9535 |
nsubj |
n,40 |
R6003 |
T9541 |
T9535 |
punct |
=,40 |
R6004 |
T9542 |
T9535 |
punct |
),40 |
R6005 |
T9543 |
T9531 |
punct |
.,are |
R6006 |
T9545 |
T9546 |
dep |
Bar,μm |
R6007 |
T9547 |
T9546 |
punct |
", ",μm |
R6008 |
T9548 |
T9546 |
nummod |
3,μm |
R6009 |
T9549 |
T9546 |
punct |
.,μm |
R6014 |
T9617 |
T9618 |
amod |
Osmotic,resistance |
R6015 |
T9618 |
T9619 |
compound |
resistance,curves |
R6016 |
T9620 |
T9619 |
prep |
of,curves |
R6017 |
T9621 |
T9622 |
amod |
red,cells |
R6018 |
T9622 |
T9620 |
pobj |
cells,of |
R6019 |
T9623 |
T9622 |
compound |
blood,cells |
R6020 |
T9624 |
T9619 |
prep |
from,curves |
R6021 |
T9625 |
T9626 |
amod |
wild,type |
R6022 |
T9626 |
T9627 |
nmod |
type,mice |
R6023 |
T9627 |
T9624 |
pobj |
mice,from |
R6024 |
T9628 |
T9626 |
cc |
and,type |
R6025 |
T9629 |
T9626 |
conj |
anxA7,type |
R6026 |
T9630 |
T9629 |
punct |
-,anxA7 |
R6027 |
T9631 |
T9629 |
punct |
/,anxA7 |
R6028 |
T9632 |
T9629 |
punct |
-,anxA7 |
R6029 |
T9633 |
T9627 |
amod |
mutant,mice |
R6030 |
T9634 |
T9619 |
punct |
.,curves |
R6031 |
T9636 |
T9637 |
det |
The,resistance |
R6032 |
T9637 |
T9639 |
nsubj |
resistance,is |
R6033 |
T9638 |
T9637 |
amod |
osmotic,resistance |
R6034 |
T9640 |
T9637 |
prep |
towards,resistance |
R6035 |
T9641 |
T9640 |
pobj |
changes,towards |
R6036 |
T9642 |
T9641 |
prep |
in,changes |
R6037 |
T9643 |
T9644 |
det |
the,strength |
R6038 |
T9644 |
T9642 |
pobj |
strength,in |
R6039 |
T9645 |
T9644 |
amod |
extracellular,strength |
R6040 |
T9646 |
T9644 |
amod |
ionic,strength |
R6041 |
T9647 |
T9648 |
det |
a,assay |
R6042 |
T9648 |
T9639 |
attr |
assay,is |
R6043 |
T9649 |
T9648 |
amod |
convenient,assay |
R6044 |
T9650 |
T9648 |
prep |
for,assay |
R6045 |
T9651 |
T9650 |
pobj |
analysis,for |
R6046 |
T9652 |
T9651 |
prep |
of,analysis |
R6047 |
T9653 |
T9654 |
det |
the,integrity |
R6048 |
T9654 |
T9652 |
pobj |
integrity,of |
R6049 |
T9655 |
T9656 |
amod |
red,cell |
R6050 |
T9656 |
T9654 |
compound |
cell,integrity |
R6051 |
T9657 |
T9656 |
compound |
blood,cell |
R6052 |
T9658 |
T9639 |
punct |
.,is |
R6053 |
T9660 |
T9661 |
nsubjpass |
It,measured |
R6054 |
T9662 |
T9661 |
auxpass |
is,measured |
R6055 |
T9663 |
T9661 |
prep |
as,measured |
R6056 |
T9664 |
T9665 |
det |
a,concentration |
R6057 |
T9665 |
T9663 |
pobj |
concentration,as |
R6058 |
T9666 |
T9667 |
compound |
sodium,chloride |
R6059 |
T9667 |
T9665 |
compound |
chloride,concentration |
R6060 |
T9668 |
T9669 |
prep |
in,starts |
R6061 |
T9669 |
T9665 |
relcl |
starts,concentration |
R6062 |
T9670 |
T9668 |
pobj |
which,in |
R6063 |
T9671 |
T9672 |
amod |
cellular,lysis |
R6064 |
T9672 |
T9669 |
nsubj |
lysis,starts |
R6065 |
T9673 |
T9674 |
punct |
(,resistance |
R6066 |
T9674 |
T9665 |
parataxis |
resistance,concentration |
R6067 |
T9675 |
T9674 |
amod |
minimal,resistance |
R6068 |
T9676 |
T9674 |
punct |
),resistance |
R6069 |
T9677 |
T9665 |
prep |
up,concentration |
R6070 |
T9678 |
T9677 |
prep |
to,up |
R6071 |
T9679 |
T9680 |
det |
a,lysis |
R6072 |
T9680 |
T9678 |
pobj |
lysis,to |
R6073 |
T9681 |
T9680 |
amod |
complete,lysis |
R6074 |
T9682 |
T9683 |
punct |
(,resistance |
R6075 |
T9683 |
T9680 |
parataxis |
resistance,lysis |
R6076 |
T9684 |
T9683 |
amod |
maximal,resistance |
R6077 |
T9685 |
T9683 |
punct |
),resistance |
R6078 |
T9686 |
T9680 |
prep |
of,lysis |
R6079 |
T9687 |
T9688 |
det |
a,cell |
R6080 |
T9688 |
T9686 |
pobj |
cell,of |
R6081 |
T9689 |
T9688 |
amod |
red,cell |
R6082 |
T9690 |
T9688 |
compound |
blood,cell |
R6083 |
T9691 |
T9661 |
punct |
.,measured |
R6084 |
T9693 |
T9694 |
det |
The,resistance |
R6085 |
T9694 |
T9696 |
nsubj |
resistance,is |
R6086 |
T9695 |
T9694 |
amod |
osmotic,resistance |
R6087 |
T9697 |
T9694 |
prep |
of,resistance |
R6088 |
T9698 |
T9699 |
compound |
annexin,A7 |
R6089 |
T9699 |
T9700 |
npadvmod |
A7,deficient |
R6090 |
T9700 |
T9701 |
amod |
deficient,cells |
R6091 |
T9701 |
T9697 |
pobj |
cells,of |
R6092 |
T9702 |
T9701 |
amod |
red,cells |
R6093 |
T9703 |
T9701 |
compound |
blood,cells |
R6094 |
T9704 |
T9705 |
advmod |
significantly,increased |
R6095 |
T9705 |
T9696 |
acomp |
increased,is |
R6096 |
T9706 |
T9696 |
prep |
compared,is |
R6097 |
T9707 |
T9706 |
prep |
to,compared |
R6098 |
T9708 |
T9709 |
det |
the,one |
R6099 |
T9709 |
T9707 |
pobj |
one,to |
R6100 |
T9710 |
T9709 |
prep |
of,one |
R6101 |
T9711 |
T9712 |
amod |
wild,type |
R6102 |
T9712 |
T9710 |
pobj |
type,of |
R6103 |
T9713 |
T9714 |
punct |
(,8 |
R6104 |
T9714 |
T9696 |
parataxis |
8,is |
R6105 |
T9715 |
T9716 |
nsubj |
p,0.00066 |
R6106 |
T9716 |
T9714 |
ccomp |
0.00066,8 |
R6107 |
T9717 |
T9716 |
punct |
=,0.00066 |
R6108 |
T9718 |
T9714 |
punct |
;,8 |
R6109 |
T9719 |
T9714 |
nsubj |
n,8 |
R6110 |
T9720 |
T9714 |
punct |
=,8 |
R6111 |
T9721 |
T9714 |
punct |
),8 |
R6112 |
T9722 |
T9696 |
punct |
.,is |
R6113 |
T9783 |
T9784 |
compound |
Platelet,aggregation |
R6114 |
T9784 |
T9785 |
compound |
aggregation,curves |
R6115 |
T9786 |
T9785 |
prep |
from,curves |
R6116 |
T9787 |
T9788 |
npadvmod |
platelet,rich |
R6117 |
T9788 |
T9789 |
amod |
rich,plasma |
R6118 |
T9789 |
T9786 |
pobj |
plasma,from |
R6119 |
T9790 |
T9789 |
prep |
of,plasma |
R6120 |
T9791 |
T9792 |
nmod |
annexin,A7 |
R6121 |
T9792 |
T9793 |
nmod |
A7,mice |
R6122 |
T9793 |
T9790 |
pobj |
mice,of |
R6123 |
T9794 |
T9795 |
compound |
knock,out |
R6124 |
T9795 |
T9792 |
appos |
out,A7 |
R6125 |
T9796 |
T9795 |
cc |
and,out |
R6126 |
T9797 |
T9798 |
amod |
wild,type |
R6127 |
T9798 |
T9795 |
conj |
type,out |
R6128 |
T9799 |
T9785 |
punct |
.,curves |
R6129 |
T9801 |
T9802 |
det |
The,values |
R6130 |
T9802 |
T9804 |
nsubjpass |
values,measured |
R6131 |
T9803 |
T9802 |
compound |
transmission,values |
R6132 |
T9805 |
T9804 |
auxpass |
were,measured |
R6133 |
T9806 |
T9804 |
prep |
with,measured |
R6134 |
T9807 |
T9808 |
det |
an,photometer |
R6135 |
T9808 |
T9806 |
pobj |
photometer,with |
R6136 |
T9809 |
T9808 |
compound |
APACT,photometer |
R6137 |
T9810 |
T9804 |
cc |
and,measured |
R6138 |
T9811 |
T9812 |
nsubjpass |
aggregation,initiated |
R6139 |
T9812 |
T9804 |
conj |
initiated,measured |
R6140 |
T9813 |
T9812 |
auxpass |
was,initiated |
R6141 |
T9814 |
T9812 |
prep |
by,initiated |
R6142 |
T9815 |
T9814 |
pcomp |
adding,by |
R6143 |
T9816 |
T9815 |
dobj |
ristocetin,adding |
R6144 |
T9817 |
T9812 |
punct |
.,initiated |
R6145 |
T9819 |
T9820 |
det |
The,seconds |
R6146 |
T9820 |
T9823 |
nsubjpass |
seconds,given |
R6147 |
T9821 |
T9820 |
amod |
first,seconds |
R6148 |
T9822 |
T9820 |
nummod |
thirty,seconds |
R6149 |
T9824 |
T9823 |
auxpass |
are,given |
R6150 |
T9825 |
T9823 |
punct |
.,given |
R6151 |
T9827 |
T9828 |
amod |
Constant,counts |
R6152 |
T9828 |
T9830 |
nsubjpass |
counts,used |
R6153 |
T9829 |
T9828 |
compound |
platelet,counts |
R6154 |
T9831 |
T9830 |
auxpass |
were,used |
R6155 |
T9832 |
T9830 |
prep |
throughout,used |
R6156 |
T9833 |
T9834 |
det |
all,experiments |
R6157 |
T9834 |
T9832 |
pobj |
experiments,throughout |
R6158 |
T9835 |
T9830 |
punct |
.,used |
R6159 |
T9837 |
T9838 |
det |
The,data |
R6160 |
T9838 |
T9841 |
nsubjpass |
data,analysed |
R6161 |
T9839 |
T9840 |
compound |
aggregation,curve |
R6162 |
T9840 |
T9838 |
compound |
curve,data |
R6163 |
T9841 |
T9843 |
ccomp |
analysed,set |
R6164 |
T9842 |
T9841 |
auxpass |
were,analysed |
R6165 |
T9844 |
T9841 |
prep |
for,analysed |
R6166 |
T9845 |
T9846 |
compound |
slope,values |
R6167 |
T9846 |
T9844 |
pobj |
values,for |
R6168 |
T9847 |
T9843 |
punct |
", ",set |
R6169 |
T9848 |
T9849 |
det |
the,amplitude |
R6170 |
T9849 |
T9843 |
nsubjpass |
amplitude,set |
R6171 |
T9850 |
T9849 |
amod |
maximal,amplitude |
R6172 |
T9851 |
T9849 |
compound |
aggregation,amplitude |
R6173 |
T9852 |
T9849 |
prep |
of,amplitude |
R6174 |
T9853 |
T9854 |
det |
every,curve |
R6175 |
T9854 |
T9852 |
pobj |
curve,of |
R6176 |
T9855 |
T9854 |
amod |
single,curve |
R6177 |
T9856 |
T9843 |
auxpass |
was,set |
R6178 |
T9857 |
T9843 |
prep |
to,set |
R6179 |
T9858 |
T9859 |
nummod |
100,% |
R6180 |
T9859 |
T9857 |
pobj |
%,to |
R6181 |
T9860 |
T9843 |
punct |
.,set |
R6182 |
T9862 |
T9863 |
det |
Both,curves |
R6183 |
T9863 |
T9865 |
nsubj |
curves,differ |
R6184 |
T9864 |
T9863 |
amod |
murine,curves |
R6185 |
T9865 |
T9866 |
ccomp |
differ,shows |
R6186 |
T9867 |
T9865 |
advmod |
significantly,differ |
R6187 |
T9868 |
T9865 |
prep |
at,differ |
R6188 |
T9869 |
T9870 |
det |
a,point |
R6189 |
T9870 |
T9868 |
pobj |
point,at |
R6190 |
T9871 |
T9870 |
compound |
time,point |
R6191 |
T9872 |
T9870 |
prep |
of,point |
R6192 |
T9873 |
T9874 |
nummod |
seven,seconds |
R6193 |
T9874 |
T9872 |
pobj |
seconds,of |
R6194 |
T9875 |
T9874 |
prep |
after,seconds |
R6195 |
T9876 |
T9877 |
compound |
platelet,initiation |
R6196 |
T9877 |
T9875 |
pobj |
initiation,after |
R6197 |
T9878 |
T9866 |
punct |
", ",shows |
R6198 |
T9879 |
T9880 |
det |
the,mutant |
R6199 |
T9880 |
T9866 |
nsubj |
mutant,shows |
R6200 |
T9881 |
T9882 |
det |
a,velocity |
R6201 |
T9882 |
T9866 |
dobj |
velocity,shows |
R6202 |
T9883 |
T9884 |
advmod |
slightly,lower |
R6203 |
T9884 |
T9882 |
amod |
lower,velocity |
R6204 |
T9885 |
T9882 |
compound |
aggregation,velocity |
R6205 |
T9886 |
T9887 |
punct |
(,transmission |
R6206 |
T9887 |
T9882 |
parataxis |
transmission,velocity |
R6207 |
T9888 |
T9889 |
nsubj |
p,0.0287 |
R6208 |
T9889 |
T9887 |
ccomp |
0.0287,transmission |
R6209 |
T9890 |
T9889 |
punct |
=,0.0287 |
R6210 |
T9891 |
T9887 |
punct |
", ",transmission |
R6211 |
T9892 |
T9893 |
compound |
n,out |
R6212 |
T9893 |
T9895 |
nsubj |
out,16 |
R6213 |
T9894 |
T9893 |
compound |
knock,out |
R6214 |
T9895 |
T9887 |
ccomp |
16,transmission |
R6215 |
T9896 |
T9895 |
punct |
=,16 |
R6216 |
T9897 |
T9887 |
punct |
", ",transmission |
R6217 |
T9898 |
T9899 |
nmod |
n,type |
R6218 |
T9899 |
T9901 |
nsubj |
type,15 |
R6219 |
T9900 |
T9899 |
amod |
wild,type |
R6220 |
T9901 |
T9887 |
ccomp |
15,transmission |
R6221 |
T9902 |
T9901 |
punct |
=,15 |
R6222 |
T9903 |
T9887 |
punct |
", ",transmission |
R6223 |
T9904 |
T9905 |
amod |
mean,out |
R6224 |
T9905 |
T9907 |
nsubj |
out,transmission |
R6225 |
T9906 |
T9905 |
compound |
knock,out |
R6226 |
T9907 |
T9887 |
ccomp |
transmission,transmission |
R6227 |
T9908 |
T9907 |
punct |
=,transmission |
R6228 |
T9909 |
T9910 |
nummod |
67.7,% |
R6229 |
T9910 |
T9907 |
compound |
%,transmission |
R6230 |
T9911 |
T9887 |
punct |
", ",transmission |
R6231 |
T9912 |
T9913 |
amod |
mean,type |
R6232 |
T9913 |
T9915 |
nsubj |
type,transmission |
R6233 |
T9914 |
T9913 |
amod |
wild,type |
R6234 |
T9915 |
T9887 |
ccomp |
transmission,transmission |
R6235 |
T9916 |
T9915 |
punct |
=,transmission |
R6236 |
T9917 |
T9918 |
nummod |
77.1,% |
R6237 |
T9918 |
T9915 |
compound |
%,transmission |
R6238 |
T9919 |
T9887 |
punct |
", ",transmission |
R6239 |
T9920 |
T9921 |
amod |
standard,error |
R6240 |
T9921 |
T9922 |
compound |
error,out |
R6241 |
T9922 |
T9924 |
nsubj |
out,transmission |
R6242 |
T9923 |
T9922 |
compound |
knock,out |
R6243 |
T9924 |
T9887 |
ccomp |
transmission,transmission |
R6244 |
T9925 |
T9924 |
punct |
=,transmission |
R6245 |
T9926 |
T9927 |
nummod |
8.3,% |
R6246 |
T9927 |
T9924 |
compound |
%,transmission |
R6247 |
T9928 |
T9887 |
punct |
", ",transmission |
R6248 |
T9929 |
T9930 |
amod |
standard,error |
R6249 |
T9930 |
T9931 |
nmod |
error,type |
R6250 |
T9931 |
T9887 |
nsubj |
type,transmission |
R6251 |
T9932 |
T9931 |
amod |
wild,type |
R6252 |
T9933 |
T9887 |
punct |
=,transmission |
R6253 |
T9934 |
T9935 |
nummod |
13.8,% |
R6254 |
T9935 |
T9887 |
compound |
%,transmission |
R6255 |
T9936 |
T9887 |
punct |
),transmission |
R6256 |
T9937 |
T9866 |
punct |
.,shows |
R6257 |
T9939 |
T9940 |
amod |
Murine,platelets |
R6258 |
T9940 |
T9941 |
nsubj |
platelets,react |
R6259 |
T9942 |
T9941 |
advmod |
immediately,react |
R6260 |
T9943 |
T9941 |
prep |
to,react |
R6261 |
T9944 |
T9945 |
det |
the,chemical |
R6262 |
T9945 |
T9943 |
pobj |
chemical,to |
R6263 |
T9946 |
T9945 |
amod |
initiating,chemical |
R6264 |
T9947 |
T9941 |
cc |
and,react |
R6265 |
T9948 |
T9941 |
conj |
show,react |
R6266 |
T9949 |
T9950 |
det |
no,phase |
R6267 |
T9950 |
T9948 |
dobj |
phase,show |
R6268 |
T9951 |
T9950 |
compound |
lag,phase |
R6269 |
T9952 |
T9948 |
prep |
like,show |
R6270 |
T9953 |
T9954 |
det |
the,platelets |
R6271 |
T9954 |
T9952 |
pobj |
platelets,like |
R6272 |
T9955 |
T9954 |
amod |
normal,platelets |
R6273 |
T9956 |
T9954 |
amod |
human,platelets |
R6274 |
T9957 |
T9941 |
punct |
.,react |
R6275 |
T9959 |
T9960 |
prep |
For,shown |
R6276 |
T9961 |
T9959 |
pobj |
comparison,For |
R6277 |
T9962 |
T9961 |
prep |
of,comparison |
R6278 |
T9963 |
T9964 |
det |
the,range |
R6279 |
T9964 |
T9962 |
pobj |
range,of |
R6280 |
T9965 |
T9964 |
prep |
of,range |
R6281 |
T9966 |
T9967 |
det |
the,impairment |
R6282 |
T9967 |
T9965 |
pobj |
impairment,of |
R6283 |
T9968 |
T9967 |
compound |
platelet,impairment |
R6284 |
T9969 |
T9970 |
advmod |
slightly,affected |
R6285 |
T9970 |
T9971 |
amod |
affected,platelets |
R6286 |
T9971 |
T9960 |
nsubjpass |
platelets,shown |
R6287 |
T9972 |
T9971 |
amod |
human,platelets |
R6288 |
T9973 |
T9960 |
auxpass |
are,shown |
R6289 |
T9974 |
T9975 |
advmod |
as,well |
R6290 |
T9975 |
T9960 |
advmod |
well,shown |
R6291 |
T9976 |
T9977 |
punct |
(,syndrome |
R6292 |
T9977 |
T9960 |
parataxis |
syndrome,shown |
R6293 |
T9978 |
T9979 |
compound |
von,Willebrand |
R6294 |
T9979 |
T9977 |
compound |
Willebrand,syndrome |
R6295 |
T9980 |
T9977 |
punct |
),syndrome |
R6296 |
T9981 |
T9960 |
punct |
.,shown |
R1408 |
T2601 |
T2599 |
pobj |
suggestion,to |
R1409 |
T2602 |
T2601 |
punct |
", ",suggestion |
R1410 |
T2603 |
T2604 |
mark |
that,plays |
R1411 |
T2604 |
T2601 |
acl |
plays,suggestion |
R1412 |
T2605 |
T2604 |
punct |
", ",plays |
R1413 |
T2606 |
T2604 |
prep |
along,plays |
R1414 |
T2607 |
T2606 |
prep |
with,along |
R1415 |
T2608 |
T2609 |
compound |
raft,domains |
R1416 |
T2609 |
T2607 |
pobj |
domains,with |
R1417 |
T2610 |
T2604 |
punct |
", ",plays |
R1418 |
T2611 |
T2612 |
compound |
annexin,A7 |
R1419 |
T2612 |
T2604 |
nsubj |
A7,plays |
R1582 |
T2787 |
T2773 |
prep |
for,both |
R1583 |
T2788 |
T2787 |
pobj |
wt,for |
R1584 |
T2789 |
T2788 |
cc |
and,wt |
R1585 |
T2790 |
T2788 |
conj |
ko,wt |
R1586 |
T2791 |
T2773 |
punct |
),both |
R1587 |
T2792 |
T2748 |
punct |
.,differ |
R1588 |
T2794 |
T2795 |
nsubj |
It,appears |
R1589 |
T2796 |
T2797 |
mark |
that,is |
R1590 |
T2797 |
T2795 |
ccomp |
is,appears |
R1591 |
T2798 |
T2799 |
compound |
annexin,A7 |
R1592 |
T2799 |
T2797 |
nsubj |
A7,is |
R1593 |
T2800 |
T2797 |
punct |
", ",is |
R1594 |
T2801 |
T2802 |
mark |
although,described |
R1595 |
T2802 |
T2797 |
advcl |
described,is |
R1649 |
T2859 |
T2860 |
aux |
To,study |
R1650 |
T2860 |
T2861 |
advcl |
study,used |
R1651 |
T2862 |
T2863 |
det |
the,distribution |
R1652 |
T2863 |
T2860 |
dobj |
distribution,study |
R1653 |
T2864 |
T2863 |
prep |
of,distribution |
R1654 |
T2865 |
T2866 |
compound |
annexin,A7 |
R1655 |
T2866 |
T2864 |
pobj |
A7,of |
R1656 |
T2867 |
T2860 |
prep |
in,study |
R1657 |
T2868 |
T2869 |
amod |
red,cells |
R1658 |
T2869 |
T2867 |
pobj |
cells,in |
R1659 |
T2870 |
T2869 |
compound |
blood,cells |
R1660 |
T2871 |
T2861 |
nsubj |
we,used |
R1661 |
T2872 |
T2873 |
npadvmod |
self,forming |
R1662 |
T2873 |
T2874 |
amod |
forming,gradients |
R1663 |
T2874 |
T2861 |
dobj |
gradients,used |
R1881 |
T3373 |
T3371 |
dobj |
diameter,had |
R1882 |
T3374 |
T3375 |
advmod |
statistically,significant |
R1883 |
T3375 |
T3373 |
amod |
significant,diameter |
R1884 |
T3376 |
T3373 |
amod |
larger,diameter |
R1885 |
T3377 |
T3373 |
compound |
cell,diameter |
R1886 |
T3378 |
T3373 |
prep |
of,diameter |
R1887 |
T3379 |
T3380 |
nummod |
6.0,μm |
R1888 |
T3380 |
T3378 |
pobj |
μm,of |
R1889 |
T3381 |
T3373 |
prep |
compared,diameter |
R1890 |
T3382 |
T3381 |
prep |
to,compared |
R1891 |
T3383 |
T3384 |
amod |
wild,type |
R1892 |
T3384 |
T3382 |
pobj |
type,to |
R1893 |
T3385 |
T3384 |
prep |
with,type |
R1894 |
T3386 |
T3387 |
nummod |
5.7,μm |
R1895 |
T3387 |
T3385 |
pobj |
μm,with |
R1896 |
T3388 |
T3389 |
punct |
(,40 |
R5956 |
T9489 |
T9490 |
amod |
Dark,field |
R5957 |
T9490 |
T9491 |
compound |
field,microscopy |
R5958 |
T9492 |
T9491 |
prep |
of,microscopy |
R5990 |
T9526 |
T9518 |
punct |
.,are |
R5991 |
T9528 |
T9529 |
det |
The,differences |
R5993 |
T9530 |
T9529 |
compound |
size,differences |
R13 |
T220 |
T219 |
compound |
blood,cells |
R19 |
T226 |
T227 |
nmod |
annexin,A7 |
R20 |
T227 |
T225 |
nmod |
A7,mouse |
R21 |
T228 |
T225 |
amod |
mutant,mouse |
R26 |
T238 |
T239 |
npadvmod |
Ca2+,binding |
R36 |
T248 |
T249 |
nummod |
47,kDa |
R2 |
T209 |
T205 |
conj |
expression,Function |
R43 |
T255 |
T254 |
auxpass |
is,thought |
R46 |
T258 |
T257 |
auxpass |
be,involved |
R55 |
T269 |
T270 |
nummod |
47,kDa |
R56 |
T270 |
T268 |
compound |
kDa,isoform |
R63 |
T277 |
T276 |
nsubjpass |
it,proposed |
R64 |
T278 |
T276 |
auxpass |
was,proposed |
R69 |
T283 |
T282 |
amod |
key,component |
R76 |
T290 |
T291 |
npadvmod |
Ca2+,dependent |
R79 |
T293 |
T289 |
compound |
vesicle,release |
R85 |
T299 |
T297 |
pobj |
which,with |
R86 |
T300 |
T301 |
amod |
red,cells |
R89 |
T303 |
T298 |
aux |
might,protect |
R97 |
T311 |
T309 |
pobj |
example,for |
R98 |
T312 |
T310 |
compound |
complement,components |
R108 |
T326 |
T325 |
compound |
blood,cells |
R115 |
T333 |
T331 |
punct |
-,anxA7 |
R116 |
T334 |
T331 |
punct |
/,anxA7 |
R117 |
T335 |
T331 |
punct |
-,anxA7 |
R123 |
T343 |
T344 |
npadvmod |
Ca2+,mediated |
R126 |
T346 |
T342 |
compound |
vesiculation,process |
R134 |
T356 |
T355 |
compound |
membrane,organization |
R137 |
T359 |
T358 |
aux |
to,disturbed |
R138 |
T360 |
T358 |
auxpass |
be,disturbed |
R155 |
T379 |
T378 |
amod |
altered,shape |
R156 |
T380 |
T378 |
compound |
cell,shape |
R160 |
T384 |
T383 |
amod |
osmotic,resistance |
R164 |
T388 |
T387 |
compound |
blood,cells |
R181 |
T409 |
T410 |
advmod |
slightly,slower |
R182 |
T410 |
T408 |
amod |
slower,velocity |
R183 |
T411 |
T408 |
compound |
aggregation,velocity |
R188 |
T416 |
T415 |
auxpass |
be,compensated |
R192 |
T420 |
T419 |
amod |
increased,number |
R203 |
T433 |
T432 |
amod |
important,role |
R215 |
T445 |
T444 |
compound |
blood,cells |
R228 |
T460 |
T459 |
compound |
membrane,cytoskeleton |
R232 |
T465 |
T464 |
compound |
blood,cells |
R236 |
T470 |
T469 |
amod |
appropriate,model |
R255 |
T493 |
T494 |
compound |
annexin,A7 |
R256 |
T494 |
T492 |
compound |
A7,isoforms |
R260 |
T498 |
T497 |
compound |
blood,cells |
R267 |
T505 |
T504 |
amod |
small,isoform |
R274 |
T514 |
T513 |
compound |
cell,types |
R292 |
T534 |
T533 |
amod |
crucial,role |
R303 |
T545 |
T544 |
compound |
cell,types |
R1328 |
T2512 |
T2513 |
det |
these,findings |
R1329 |
T2513 |
T2509 |
dobj |
findings,extended |
R1330 |
T2514 |
T2509 |
cc |
and,extended |
R1331 |
T2515 |
T2509 |
conj |
show,extended |
R1332 |
T2516 |
T2515 |
advmod |
here,show |
R1333 |
T2517 |
T2518 |
mark |
that,is |
R1334 |
T2518 |
T2515 |
ccomp |
is,show |
R1402 |
T2594 |
T2595 |
poss |
Its,presence |
R1403 |
T2595 |
T2596 |
nsubj |
presence,led |
R1404 |
T2597 |
T2595 |
prep |
in,presence |
R1405 |
T2598 |
T2597 |
pobj |
vesicles,in |
R1406 |
T2599 |
T2596 |
prep |
to,led |
R1407 |
T2600 |
T2601 |
det |
the,suggestion |
R1897 |
T3389 |
T3373 |
parataxis |
40,diameter |
R1898 |
T3390 |
T3391 |
nsubj |
p,0.015 |
R1899 |
T3391 |
T3389 |
ccomp |
0.015,40 |
R1900 |
T3392 |
T3391 |
punct |
=,0.015 |
R1901 |
T3393 |
T3389 |
punct |
", ",40 |
R1941 |
T3437 |
T3438 |
nsubj |
Shape,influence |
R1942 |
T3439 |
T3437 |
cc |
and,Shape |
R1943 |
T3440 |
T3437 |
conj |
MCV,Shape |
R1944 |
T3441 |
T3437 |
prep |
of,Shape |
R1945 |
T3442 |
T3443 |
det |
a,cell |
R1946 |
T3443 |
T3441 |
pobj |
cell,of |
R1947 |
T3444 |
T3443 |
amod |
red,cell |
R1948 |
T3445 |
T3443 |
compound |
blood,cell |
R1949 |
T3446 |
T3438 |
advmod |
essentially,influence |
R1950 |
T3447 |
T3448 |
poss |
its,function |
R1951 |
T3448 |
T3438 |
dobj |
function,influence |
R1952 |
T3449 |
T3448 |
cc |
and,function |
R1953 |
T3450 |
T3451 |
amod |
capillary,passage |
R1954 |
T3451 |
T3448 |
conj |
passage,function |
R1955 |
T3452 |
T3438 |
punct |
.,influence |
R1956 |
T3454 |
T3455 |
det |
The,form |
R1957 |
T3455 |
T3458 |
nsubj |
form,depends |
R1958 |
T3456 |
T3455 |
amod |
typical,form |
R1959 |
T3457 |
T3455 |
compound |
biconcave,form |
R1960 |
T3459 |
T3458 |
prep |
on,depends |
R1961 |
T3460 |
T3461 |
amod |
various,influences |
R1962 |
T3461 |
T3459 |
pobj |
influences,on |
R1963 |
T3462 |
T3461 |
prep |
including,influences |
R1964 |
T3463 |
T3464 |
det |
the,composition |
R1965 |
T3464 |
T3462 |
pobj |
composition,including |
R1966 |
T3465 |
T3466 |
compound |
membrane,lipid |
R1967 |
T3466 |
T3464 |
compound |
lipid,composition |
R1968 |
T3467 |
T3464 |
cc |
and,composition |
R1969 |
T3468 |
T3469 |
det |
the,cytoskeleton |
R1970 |
T3469 |
T3464 |
conj |
cytoskeleton,composition |
R1971 |
T3470 |
T3469 |
amod |
submembranous,cytoskeleton |
R1972 |
T3471 |
T3472 |
punct |
[,32 |
R1973 |
T3472 |
T3469 |
parataxis |
32,cytoskeleton |
R1974 |
T3473 |
T3472 |
punct |
],32 |
R2231 |
T3752 |
T3753 |
poss |
their,morphology |
R2232 |
T3753 |
T3751 |
pobj |
morphology,with |
R2233 |
T3754 |
T3738 |
punct |
.,tried |
R2234 |
T3756 |
T3757 |
nsubj |
We,observe |
R2235 |
T3758 |
T3757 |
aux |
could,observe |
R2236 |
T3759 |
T3757 |
neg |
not,observe |
R2237 |
T3760 |
T3761 |
det |
any,difference |
R2238 |
T3761 |
T3757 |
dobj |
difference,observe |
R2239 |
T3762 |
T3763 |
advmod |
statistically,significant |
R2240 |
T3763 |
T3761 |
amod |
significant,difference |
R2241 |
T3764 |
T3761 |
prep |
of,difference |
R2242 |
T3765 |
T3764 |
pobj |
values,of |
R2243 |
T3766 |
T3765 |
acl |
describing,values |
R2244 |
T3767 |
T3768 |
compound |
membrane,rigidity |
R2245 |
T3768 |
T3766 |
dobj |
rigidity,describing |
R2246 |
T3769 |
T3768 |
cc |
and,rigidity |
R2247 |
T3770 |
T3771 |
compound |
lysis,force |
R2248 |
T3771 |
T3768 |
conj |
force,rigidity |
R2249 |
T3772 |
T3757 |
prep |
in,observe |
R1215 |
T2129 |
T2128 |
compound |
lipid,rafts |
R3918 |
T6487 |
T6488 |
det |
the,absence |