PMC:1942070 / 4119-4528
Annnotations
pmc-enju-pas
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bionlp-st-ge-2016-spacy-parsed
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sIgM staining\nDT40 B cells (2 × 106 cells per point) were resuspended in 200 μl buffer (RPMI 1640 media, 1% foetal calf serum) containing anti-chicken M1 monoclonal antibody conjugated to FITC for 20 min on ice. 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UBERON-AE
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GO-MF
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GO-CC
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sentences
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bionlp-st-ge-2016-reference-tees
{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T2020","span":{"begin":143,"end":178},"obj":"Protein"}],"text":"2.2 sIgM staining\nDT40 B cells (2 × 106 cells per point) were resuspended in 200 μl buffer (RPMI 1640 media, 1% foetal calf serum) containing anti-chicken M1 monoclonal antibody conjugated to FITC for 20 min on ice. The cells were washed twice and fluorescent intensity was analysed by flow cytometry.\nAll results shown are representative of at two to four independent experiments unless otherwise indicated."}