PMC:1942070 / 3726-4117 JSONTXT

{"project":"2_test","denotations":[{"id":"17349631-16449666-30580532","span":{"begin":196,"end":197},"obj":"16449666"},{"id":"17349631-16449666-30580533","span":{"begin":309,"end":310},"obj":"16449666"},{"id":"17349631-7730364-30580534","span":{"begin":387,"end":389},"obj":"7730364"}],"text":"2.1 Cell culture, transient transfections and cell stimulation\nThe generation, culture and activation of PKD1−/−, PKD3−/− and PKD1/3−/− knockout DT40 B cell lines have been described previously [1]. Cells were lysed and protein extracts were analysed in Western blotting experiments as previously described [1]. Chloramphenicol acetyl transferase assays have been described previously [29]."}

{"project":"MyTest","denotations":[{"id":"17349631-16449666-30580532","span":{"begin":196,"end":197},"obj":"16449666"},{"id":"17349631-16449666-30580533","span":{"begin":309,"end":310},"obj":"16449666"},{"id":"17349631-7730364-30580534","span":{"begin":387,"end":389},"obj":"7730364"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"2.1 Cell culture, transient transfections and cell stimulation\nThe generation, culture and activation of PKD1−/−, PKD3−/− and PKD1/3−/− knockout DT40 B cell lines have been described previously [1]. Cells were lysed and protein extracts were analysed in Western blotting experiments as previously described [1]. Chloramphenicol acetyl transferase assays have been described previously [29]."}

{"project":"pmc-enju-pas","denotations":[{"id":"T1675","span":{"begin":10,"end":17},"obj":"NN"},{"id":"T1676","span":{"begin":17,"end":18},"obj":"-COMMA-"},{"id":"T1677","span":{"begin":19,"end":28},"obj":"JJ"},{"id":"T1678","span":{"begin":29,"end":42},"obj":"NN"},{"id":"T1679","span":{"begin":43,"end":46},"obj":"CC"},{"id":"T1680","span":{"begin":47,"end":51},"obj":"NN"},{"id":"T1681","span":{"begin":52,"end":63},"obj":"NN"},{"id":"T1682","span":{"begin":64,"end":67},"obj":"DT"},{"id":"T1683","span":{"begin":68,"end":78},"obj":"NN"},{"id":"T1684","span":{"begin":78,"end":79},"obj":"-COMMA-"},{"id":"T1685","span":{"begin":80,"end":87},"obj":"NN"},{"id":"T1686","span":{"begin":88,"end":91},"obj":"CC"},{"id":"T1687","span":{"begin":92,"end":102},"obj":"NN"},{"id":"T1688","span":{"begin":103,"end":105},"obj":"IN"},{"id":"T1689","span":{"begin":106,"end":113},"obj":"NN"},{"id":"T1690","span":{"begin":113,"end":114},"obj":"-COMMA-"},{"id":"T1691","span":{"begin":115,"end":122},"obj":"NN"},{"id":"T1692","span":{"begin":123,"end":126},"obj":"CC"},{"id":"T1693","span":{"begin":127,"end":136},"obj":"NN"},{"id":"T1694","span":{"begin":137,"end":145},"obj":"NN"},{"id":"T1695","span":{"begin":146,"end":150},"obj":"NN"},{"id":"T1696","span":{"begin":151,"end":152},"obj":"NN"},{"id":"T1697","span":{"begin":153,"end":157},"obj":"NN"},{"id":"T1698","span":{"begin":158,"end":163},"obj":"NN"},{"id":"T1699","span":{"begin":164,"end":168},"obj":"VB"},{"id":"T1700","span":{"begin":169,"end":173},"obj":"VB"},{"id":"T1701","span":{"begin":174,"end":183},"obj":"VB"},{"id":"T1702","span":{"begin":184,"end":194},"obj":"RB"},{"id":"T1703","span":{"begin":195,"end":196},"obj":"-LRB-"},{"id":"T1704","span":{"begin":196,"end":197},"obj":"CD"},{"id":"T1705","span":{"begin":197,"end":198},"obj":"-RRB-"},{"id":"T1706","span":{"begin":200,"end":205},"obj":"NN"},{"id":"T1707","span":{"begin":206,"end":210},"obj":"VB"},{"id":"T1708","span":{"begin":211,"end":216},"obj":"VB"},{"id":"T1709","span":{"begin":217,"end":220},"obj":"CC"},{"id":"T1710","span":{"begin":221,"end":228},"obj":"NN"},{"id":"T1711","span":{"begin":229,"end":237},"obj":"NN"},{"id":"T1712","span":{"begin":238,"end":242},"obj":"VB"},{"id":"T1713","span":{"begin":243,"end":251},"obj":"VB"},{"id":"T1714","span":{"begin":252,"end":254},"obj":"IN"},{"id":"T1715","span":{"begin":255,"end":262},"obj":"JJ"},{"id":"T1716","span":{"begin":263,"end":271},"obj":"NN"},{"id":"T1717","span":{"begin":272,"end":283},"obj":"NN"},{"id":"T1718","span":{"begin":284,"end":286},"obj":"IN"},{"id":"T1719","span":{"begin":287,"end":297},"obj":"RB"},{"id":"T1720","span":{"begin":298,"end":307},"obj":"VB"},{"id":"T1721","span":{"begin":308,"end":309},"obj":"-LRB-"},{"id":"T1722","span":{"begin":309,"end":310},"obj":"CD"},{"id":"T1723","span":{"begin":310,"end":311},"obj":"-RRB-"},{"id":"T1724","span":{"begin":313,"end":328},"obj":"NN"},{"id":"T1725","span":{"begin":329,"end":335},"obj":"NN"},{"id":"T1726","span":{"begin":336,"end":347},"obj":"NN"},{"id":"T1727","span":{"begin":348,"end":354},"obj":"NN"},{"id":"T1728","span":{"begin":355,"end":359},"obj":"VB"},{"id":"T1729","span":{"begin":360,"end":364},"obj":"VB"},{"id":"T1730","span":{"begin":365,"end":374},"obj":"VB"},{"id":"T1731","span":{"begin":375,"end":385},"obj":"RB"},{"id":"T1732","span":{"begin":386,"end":387},"obj":"-LRB-"},{"id":"T1733","span":{"begin":387,"end":389},"obj":"CD"},{"id":"T1734","span":{"begin":389,"end":390},"obj":"-RRB-"}],"relations":[{"id":"R1438","pred":"arg1Of","subj":"T1675","obj":"T1676"},{"id":"R1439","pred":"arg1Of","subj":"T1676","obj":"T1679"},{"id":"R1440","pred":"arg2Of","subj":"T1678","obj":"T1676"},{"id":"R1441","pred":"arg1Of","subj":"T1678","obj":"T1677"},{"id":"R1442","pred":"arg2Of","subj":"T1681","obj":"T1679"},{"id":"R1443","pred":"arg1Of","subj":"T1681","obj":"T1680"},{"id":"R1444","pred":"arg1Of","subj":"T1683","obj":"T1684"},{"id":"R1445","pred":"arg1Of","subj":"T1684","obj":"T1686"},{"id":"R1446","pred":"arg2Of","subj":"T1685","obj":"T1684"},{"id":"R1447","pred":"arg1Of","subj":"T1686","obj":"T1682"},{"id":"R1448","pred":"arg1Of","subj":"T1686","obj":"T1688"},{"id":"R1449","pred":"arg1Of","subj":"T1686","obj":"T1699"},{"id":"R1450","pred":"arg1Of","subj":"T1686","obj":"T1700"},{"id":"R1451","pred":"arg2Of","subj":"T1686","obj":"T1701"},{"id":"R1452","pred":"arg2Of","subj":"T1687","obj":"T1686"},{"id":"R1453","pred":"arg1Of","subj":"T1689","obj":"T1690"},{"id":"R1454","pred":"arg1Of","subj":"T1690","obj":"T1692"},{"id":"R1455","pred":"arg2Of","subj":"T1691","obj":"T1690"},{"id":"R1456","pred":"arg2Of","subj":"T1693","obj":"T1692"},{"id":"R1457","pred":"arg2Of","subj":"T1698","obj":"T1688"},{"id":"R1458","pred":"arg1Of","subj":"T1698","obj":"T1689"},{"id":"R1459","pred":"arg1Of","subj":"T1698","obj":"T1691"},{"id":"R1460","pred":"arg1Of","subj":"T1698","obj":"T1693"},{"id":"R1461","pred":"arg1Of","subj":"T1698","obj":"T1694"},{"id":"R1462","pred":"arg1Of","subj":"T1698","obj":"T1695"},{"id":"R1463","pred":"arg1Of","subj":"T1698","obj":"T1696"},{"id":"R1464","pred":"arg1Of","subj":"T1698","obj":"T1697"},{"id":"R1465","pred":"arg2Of","subj":"T1701","obj":"T1699"},{"id":"R1466","pred":"arg2Of","subj":"T1701","obj":"T1700"},{"id":"R1467","pred":"arg1Of","subj":"T1701","obj":"T1702"},{"id":"R1468","pred":"arg1Of","subj":"T1701","obj":"T1703"},{"id":"R1469","pred":"arg2Of","subj":"T1704","obj":"T1703"},{"id":"R1470","pred":"arg3Of","subj":"T1705","obj":"T1703"},{"id":"R1471","pred":"arg1Of","subj":"T1706","obj":"T1707"},{"id":"R1472","pred":"arg2Of","subj":"T1706","obj":"T1708"},{"id":"R1473","pred":"arg2Of","subj":"T1708","obj":"T1707"},{"id":"R1474","pred":"arg1Of","subj":"T1708","obj":"T1709"},{"id":"R1475","pred":"arg1Of","subj":"T1711","obj":"T1710"},{"id":"R1476","pred":"arg1Of","subj":"T1711","obj":"T1712"},{"id":"R1477","pred":"arg2Of","subj":"T1711","obj":"T1713"},{"id":"R1478","pred":"arg2Of","subj":"T1713","obj":"T1709"},{"id":"R1479","pred":"arg2Of","subj":"T1713","obj":"T1712"},{"id":"R1480","pred":"arg1Of","subj":"T1713","obj":"T1714"},{"id":"R1481","pred":"arg1Of","subj":"T1713","obj":"T1718"},{"id":"R1482","pred":"arg2Of","subj":"T1717","obj":"T1714"},{"id":"R1483","pred":"arg1Of","subj":"T1717","obj":"T1715"},{"id":"R1484","pred":"arg1Of","subj":"T1717","obj":"T1716"},{"id":"R1485","pred":"arg1Of","subj":"T1720","obj":"T1719"},{"id":"R1486","pred":"arg2Of","subj":"T1722","obj":"T1718"},{"id":"R1487","pred":"arg1Of","subj":"T1722","obj":"T1720"},{"id":"R1488","pred":"arg2Of","subj":"T1722","obj":"T1721"},{"id":"R1489","pred":"arg3Of","subj":"T1723","obj":"T1721"},{"id":"R1490","pred":"arg1Of","subj":"T1727","obj":"T1724"},{"id":"R1491","pred":"arg1Of","subj":"T1727","obj":"T1725"},{"id":"R1492","pred":"arg1Of","subj":"T1727","obj":"T1726"},{"id":"R1493","pred":"arg1Of","subj":"T1727","obj":"T1728"},{"id":"R1494","pred":"arg1Of","subj":"T1727","obj":"T1729"},{"id":"R1495","pred":"arg2Of","subj":"T1727","obj":"T1730"},{"id":"R1496","pred":"arg2Of","subj":"T1730","obj":"T1728"},{"id":"R1497","pred":"arg2Of","subj":"T1730","obj":"T1729"},{"id":"R1498","pred":"arg1Of","subj":"T1730","obj":"T1731"},{"id":"R1499","pred":"arg1Of","subj":"T1730","obj":"T1732"},{"id":"R1500","pred":"arg2Of","subj":"T1733","obj":"T1732"},{"id":"R1501","pred":"arg3Of","subj":"T1734","obj":"T1732"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"2.1 Cell culture, transient transfections and cell stimulation\nThe generation, culture and activation of PKD1−/−, PKD3−/− and PKD1/3−/− knockout DT40 B cell lines have been described previously [1]. Cells were lysed and protein extracts were analysed in Western blotting experiments as previously described [1]. Chloramphenicol acetyl transferase assays have been described previously [29]."}

{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T1743","span":{"begin":5,"end":9},"obj":"NNP"},{"id":"T1744","span":{"begin":10,"end":17},"obj":"NN"},{"id":"T1745","span":{"begin":17,"end":18},"obj":","},{"id":"T1746","span":{"begin":19,"end":28},"obj":"NN"},{"id":"T1747","span":{"begin":29,"end":42},"obj":"NNS"},{"id":"T1748","span":{"begin":43,"end":46},"obj":"CC"},{"id":"T1749","span":{"begin":47,"end":51},"obj":"NN"},{"id":"T1750","span":{"begin":52,"end":63},"obj":"NN"},{"id":"T1751","span":{"begin":64,"end":67},"obj":"DT"},{"id":"T1752","span":{"begin":68,"end":78},"obj":"NN"},{"id":"T1753","span":{"begin":78,"end":79},"obj":","},{"id":"T1754","span":{"begin":80,"end":87},"obj":"NN"},{"id":"T1755","span":{"begin":88,"end":91},"obj":"CC"},{"id":"T1756","span":{"begin":92,"end":102},"obj":"NN"},{"id":"T1757","span":{"begin":103,"end":105},"obj":"IN"},{"id":"T1758","span":{"begin":106,"end":110},"obj":"CD"},{"id":"T1759","span":{"begin":110,"end":111},"obj":"CD"},{"id":"T1760","span":{"begin":111,"end":112},"obj":"NN"},{"id":"T1761","span":{"begin":112,"end":113},"obj":"NN"},{"id":"T1762","span":{"begin":113,"end":114},"obj":","},{"id":"T1763","span":{"begin":115,"end":119},"obj":"NNP"},{"id":"T1764","span":{"begin":119,"end":120},"obj":"NNP"},{"id":"T1765","span":{"begin":120,"end":121},"obj":"VBD"},{"id":"T1766","span":{"begin":121,"end":122},"obj":"CD"},{"id":"T1767","span":{"begin":123,"end":126},"obj":"CC"},{"id":"T1768","span":{"begin":127,"end":133},"obj":"CD"},{"id":"T1769","span":{"begin":133,"end":134},"obj":"CD"},{"id":"T1770","span":{"begin":134,"end":135},"obj":"NN"},{"id":"T1771","span":{"begin":135,"end":136},"obj":"NN"},{"id":"T1772","span":{"begin":137,"end":145},"obj":"NN"},{"id":"T1773","span":{"begin":146,"end":150},"obj":"NNP"},{"id":"T1774","span":{"begin":151,"end":152},"obj":"NNP"},{"id":"T1775","span":{"begin":153,"end":157},"obj":"NN"},{"id":"T1776","span":{"begin":158,"end":163},"obj":"NNS"},{"id":"T1777","span":{"begin":164,"end":168},"obj":"VBP"},{"id":"T1778","span":{"begin":169,"end":173},"obj":"VBN"},{"id":"T1779","span":{"begin":174,"end":183},"obj":"VBN"},{"id":"T1780","span":{"begin":184,"end":194},"obj":"RB"},{"id":"T1781","span":{"begin":195,"end":196},"obj":"NNP"},{"id":"T1782","span":{"begin":196,"end":197},"obj":"CD"},{"id":"T1783","span":{"begin":197,"end":198},"obj":"NNP"},{"id":"T1784","span":{"begin":198,"end":199},"obj":"."},{"id":"T1785","span":{"begin":200,"end":205},"obj":"NNS"},{"id":"T1786","span":{"begin":206,"end":210},"obj":"VBD"},{"id":"T1787","span":{"begin":211,"end":216},"obj":"VBN"},{"id":"T1788","span":{"begin":217,"end":220},"obj":"CC"},{"id":"T1789","span":{"begin":221,"end":228},"obj":"NN"},{"id":"T1790","span":{"begin":229,"end":237},"obj":"NNS"},{"id":"T1791","span":{"begin":238,"end":242},"obj":"VBD"},{"id":"T1792","span":{"begin":243,"end":251},"obj":"VBN"},{"id":"T1793","span":{"begin":252,"end":254},"obj":"IN"},{"id":"T1794","span":{"begin":255,"end":262},"obj":"JJ"},{"id":"T1795","span":{"begin":263,"end":271},"obj":"VBG"},{"id":"T1796","span":{"begin":272,"end":283},"obj":"NNS"},{"id":"T1797","span":{"begin":284,"end":286},"obj":"IN"},{"id":"T1798","span":{"begin":287,"end":297},"obj":"RB"},{"id":"T1799","span":{"begin":298,"end":307},"obj":"VBN"},{"id":"T1800","span":{"begin":308,"end":309},"obj":"NNP"},{"id":"T1801","span":{"begin":309,"end":310},"obj":"CD"},{"id":"T1802","span":{"begin":310,"end":311},"obj":"NNP"},{"id":"T1803","span":{"begin":311,"end":312},"obj":"."},{"id":"T1804","span":{"begin":313,"end":328},"obj":"NNP"},{"id":"T1805","span":{"begin":329,"end":335},"obj":"NN"},{"id":"T1806","span":{"begin":336,"end":347},"obj":"NN"},{"id":"T1807","span":{"begin":348,"end":354},"obj":"NNS"},{"id":"T1808","span":{"begin":355,"end":359},"obj":"VBP"},{"id":"T1809","span":{"begin":360,"end":364},"obj":"VBN"},{"id":"T1810","span":{"begin":365,"end":374},"obj":"VBN"},{"id":"T1811","span":{"begin":375,"end":385},"obj":"RB"},{"id":"T1812","span":{"begin":386,"end":387},"obj":"NNP"},{"id":"T1813","span":{"begin":387,"end":389},"obj":"CD"},{"id":"T1814","span":{"begin":389,"end":390},"obj":"NNP"},{"id":"T1815","span":{"begin":390,"end":391},"obj":"."}],"relations":[{"id":"R1519","pred":"nummod","subj":"T1759","obj":"T1761"},{"id":"R1520","pred":"compound","subj":"T1760","obj":"T1761"},{"id":"R1521","pred":"pobj","subj":"T1761","obj":"T1757"},{"id":"R1522","pred":"punct","subj":"T1762","obj":"T1765"},{"id":"R1523","pred":"compound","subj":"T1763","obj":"T1764"},{"id":"R1524","pred":"nsubj","subj":"T1764","obj":"T1765"},{"id":"R1525","pred":"ROOT","subj":"T1765","obj":"T1765"},{"id":"R1526","pred":"nummod","subj":"T1766","obj":"T1776"},{"id":"R1527","pred":"cc","subj":"T1767","obj":"T1766"},{"id":"R1528","pred":"nummod","subj":"T1768","obj":"T1776"},{"id":"R1529","pred":"nummod","subj":"T1769","obj":"T1776"},{"id":"R1530","pred":"nmod","subj":"T1770","obj":"T1772"},{"id":"R1531","pred":"compound","subj":"T1771","obj":"T1772"},{"id":"R1532","pred":"compound","subj":"T1772","obj":"T1776"},{"id":"R1533","pred":"compound","subj":"T1773","obj":"T1774"},{"id":"R1534","pred":"compound","subj":"T1774","obj":"T1775"},{"id":"R1535","pred":"compound","subj":"T1775","obj":"T1776"},{"id":"R1536","pred":"nsubjpass","subj":"T1776","obj":"T1779"},{"id":"R1537","pred":"aux","subj":"T1777","obj":"T1779"},{"id":"R1538","pred":"auxpass","subj":"T1778","obj":"T1779"},{"id":"R1539","pred":"ccomp","subj":"T1779","obj":"T1765"},{"id":"R1540","pred":"advmod","subj":"T1780","obj":"T1783"},{"id":"R1541","pred":"nmod","subj":"T1781","obj":"T1783"},{"id":"R1542","pred":"nummod","subj":"T1782","obj":"T1781"},{"id":"R1543","pred":"dobj","subj":"T1783","obj":"T1779"},{"id":"R1544","pred":"punct","subj":"T1784","obj":"T1765"},{"id":"R1545","pred":"nsubjpass","subj":"T1785","obj":"T1787"},{"id":"R1546","pred":"auxpass","subj":"T1786","obj":"T1787"},{"id":"R1547","pred":"ROOT","subj":"T1787","obj":"T1787"},{"id":"R1548","pred":"cc","subj":"T1788","obj":"T1787"},{"id":"R1549","pred":"compound","subj":"T1789","obj":"T1790"},{"id":"R1550","pred":"nsubjpass","subj":"T1790","obj":"T1792"},{"id":"R1551","pred":"auxpass","subj":"T1791","obj":"T1792"},{"id":"R1552","pred":"conj","subj":"T1792","obj":"T1787"},{"id":"R1553","pred":"prep","subj":"T1793","obj":"T1792"},{"id":"R1554","pred":"amod","subj":"T1794","obj":"T1796"},{"id":"R1555","pred":"amod","subj":"T1795","obj":"T1796"},{"id":"R1556","pred":"pobj","subj":"T1796","obj":"T1793"},{"id":"R1557","pred":"mark","subj":"T1797","obj":"T1799"},{"id":"R1558","pred":"advmod","subj":"T1798","obj":"T1799"},{"id":"R1559","pred":"advcl","subj":"T1799","obj":"T1792"},{"id":"R1560","pred":"nmod","subj":"T1800","obj":"T1802"},{"id":"R1561","pred":"nummod","subj":"T1801","obj":"T1802"},{"id":"R1562","pred":"dobj","subj":"T1802","obj":"T1799"},{"id":"R1563","pred":"punct","subj":"T1803","obj":"T1792"},{"id":"R1564","pred":"nmod","subj":"T1804","obj":"T1807"},{"id":"R1565","pred":"nmod","subj":"T1805","obj":"T1807"},{"id":"R1566","pred":"compound","subj":"T1806","obj":"T1807"},{"id":"R1567","pred":"nsubjpass","subj":"T1807","obj":"T1810"},{"id":"R1568","pred":"aux","subj":"T1808","obj":"T1810"},{"id":"R1569","pred":"auxpass","subj":"T1809","obj":"T1810"},{"id":"R1570","pred":"ROOT","subj":"T1810","obj":"T1810"},{"id":"R1571","pred":"advmod","subj":"T1811","obj":"T1814"},{"id":"R1572","pred":"nmod","subj":"T1812","obj":"T1814"},{"id":"R1573","pred":"nummod","subj":"T1813","obj":"T1814"},{"id":"R1574","pred":"dobj","subj":"T1814","obj":"T1810"},{"id":"R1575","pred":"punct","subj":"T1815","obj":"T1810"},{"id":"R1503","pred":"compound","subj":"T1743","obj":"T1744"},{"id":"R1504","pred":"ROOT","subj":"T1744","obj":"T1744"},{"id":"R1505","pred":"punct","subj":"T1745","obj":"T1744"},{"id":"R1506","pred":"compound","subj":"T1746","obj":"T1747"},{"id":"R1507","pred":"appos","subj":"T1747","obj":"T1744"},{"id":"R1508","pred":"cc","subj":"T1748","obj":"T1747"},{"id":"R1509","pred":"compound","subj":"T1749","obj":"T1750"},{"id":"R1510","pred":"conj","subj":"T1750","obj":"T1747"},{"id":"R1511","pred":"det","subj":"T1751","obj":"T1752"},{"id":"R1512","pred":"nsubj","subj":"T1752","obj":"T1765"},{"id":"R1513","pred":"punct","subj":"T1753","obj":"T1752"},{"id":"R1514","pred":"conj","subj":"T1754","obj":"T1752"},{"id":"R1515","pred":"cc","subj":"T1755","obj":"T1754"},{"id":"R1516","pred":"conj","subj":"T1756","obj":"T1754"},{"id":"R1517","pred":"prep","subj":"T1757","obj":"T1752"},{"id":"R1518","pred":"nummod","subj":"T1758","obj":"T1761"}],"text":"2.1 Cell culture, transient transfections and cell stimulation\nThe generation, culture and activation of PKD1−/−, PKD3−/− and PKD1/3−/− knockout DT40 B cell lines have been described previously [1]. Cells were lysed and protein extracts were analysed in Western blotting experiments as previously described [1]. Chloramphenicol acetyl transferase assays have been described previously [29]."}

{"project":"GO-CC","denotations":[{"id":"T1816","span":{"begin":47,"end":51},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1817","span":{"begin":153,"end":157},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"2.1 Cell culture, transient transfections and cell stimulation\nThe generation, culture and activation of PKD1−/−, PKD3−/− and PKD1/3−/− knockout DT40 B cell lines have been described previously [1]. Cells were lysed and protein extracts were analysed in Western blotting experiments as previously described [1]. Chloramphenicol acetyl transferase assays have been described previously [29]."}

{"project":"sentences","denotations":[{"id":"T1671","span":{"begin":64,"end":199},"obj":"Sentence"},{"id":"T1672","span":{"begin":200,"end":312},"obj":"Sentence"},{"id":"T1673","span":{"begin":313,"end":391},"obj":"Sentence"},{"id":"T28","span":{"begin":0,"end":63},"obj":"Sentence"},{"id":"T29","span":{"begin":64,"end":199},"obj":"Sentence"},{"id":"T30","span":{"begin":200,"end":312},"obj":"Sentence"},{"id":"T31","span":{"begin":313,"end":391},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"2.1 Cell culture, transient transfections and cell stimulation\nThe generation, culture and activation of PKD1−/−, PKD3−/− and PKD1/3−/− knockout DT40 B cell lines have been described previously [1]. Cells were lysed and protein extracts were analysed in Western blotting experiments as previously described [1]. Chloramphenicol acetyl transferase assays have been described previously [29]."}

{"project":"events-check-again","denotations":[{"id":"T1851","span":{"begin":106,"end":110},"obj":"Protein"},{"id":"T1852","span":{"begin":115,"end":119},"obj":"Protein"},{"id":"T1853","span":{"begin":127,"end":131},"obj":"Protein"},{"id":"T1854","span":{"begin":132,"end":133},"obj":"Protein"},{"id":"T1855","span":{"begin":137,"end":145},"obj":"Negative_regulation"},{"id":"T1856","span":{"begin":137,"end":145},"obj":"Negative_regulation"},{"id":"T1857","span":{"begin":137,"end":145},"obj":"Negative_regulation"},{"id":"T1858","span":{"begin":137,"end":145},"obj":"Negative_regulation"}],"relations":[{"id":"R1592","pred":"themeOf","subj":"T1851","obj":"T1855"},{"id":"R1593","pred":"themeOf","subj":"T1852","obj":"T1856"},{"id":"R1594","pred":"themeOf","subj":"T1853","obj":"T1857"},{"id":"R1595","pred":"themeOf","subj":"T1854","obj":"T1858"}],"text":"2.1 Cell culture, transient transfections and cell stimulation\nThe generation, culture and activation of PKD1−/−, PKD3−/− and PKD1/3−/− knockout DT40 B cell lines have been described previously [1]. Cells were lysed and protein extracts were analysed in Western blotting experiments as previously described [1]. Chloramphenicol acetyl transferase assays have been described previously [29]."}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T1859","span":{"begin":106,"end":110},"obj":"Protein"},{"id":"T1860","span":{"begin":115,"end":119},"obj":"Protein"},{"id":"T1861","span":{"begin":127,"end":131},"obj":"Protein"},{"id":"T1862","span":{"begin":313,"end":347},"obj":"Protein"}],"text":"2.1 Cell culture, transient transfections and cell stimulation\nThe generation, culture and activation of PKD1−/−, PKD3−/− and PKD1/3−/− knockout DT40 B cell lines have been described previously [1]. Cells were lysed and protein extracts were analysed in Western blotting experiments as previously described [1]. Chloramphenicol acetyl transferase assays have been described previously [29]."}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T1662","span":{"begin":106,"end":110},"obj":"Protein"},{"id":"T1663","span":{"begin":115,"end":119},"obj":"Protein"},{"id":"T1664","span":{"begin":127,"end":131},"obj":"Protein"},{"id":"T1665","span":{"begin":132,"end":133},"obj":"Protein"},{"id":"T1666","span":{"begin":137,"end":145},"obj":"Negative_regulation"},{"id":"T1667","span":{"begin":137,"end":145},"obj":"Negative_regulation"},{"id":"T1668","span":{"begin":137,"end":145},"obj":"Negative_regulation"},{"id":"T1669","span":{"begin":137,"end":145},"obj":"Negative_regulation"}],"relations":[{"id":"R1433","pred":"themeOf","subj":"T1662","obj":"T1666"},{"id":"R1434","pred":"themeOf","subj":"T1663","obj":"T1667"},{"id":"R1435","pred":"themeOf","subj":"T1664","obj":"T1668"},{"id":"R1436","pred":"themeOf","subj":"T1665","obj":"T1669"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"2.1 Cell culture, transient transfections and cell stimulation\nThe generation, culture and activation of PKD1−/−, PKD3−/− and PKD1/3−/− knockout DT40 B cell lines have been described previously [1]. Cells were lysed and protein extracts were analysed in Western blotting experiments as previously described [1]. Chloramphenicol acetyl transferase assays have been described previously [29]."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T1737","span":{"begin":127,"end":131},"obj":"P98161"},{"id":"T1735","span":{"begin":106,"end":110},"obj":"P98161"},{"id":"T1736","span":{"begin":115,"end":119},"obj":"Q99853"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"2.1 Cell culture, transient transfections and cell stimulation\nThe generation, culture and activation of PKD1−/−, PKD3−/− and PKD1/3−/− knockout DT40 B cell lines have been described previously [1]. Cells were lysed and protein extracts were analysed in Western blotting experiments as previously described [1]. Chloramphenicol acetyl transferase assays have been described previously [29]."}

{"project":"test2","denotations":[{"id":"T1658","span":{"begin":106,"end":110},"obj":"Protein"},{"id":"T1659","span":{"begin":115,"end":119},"obj":"Protein"},{"id":"T1660","span":{"begin":127,"end":131},"obj":"Protein"},{"id":"T1661","span":{"begin":132,"end":133},"obj":"Protein"}],"text":"2.1 Cell culture, transient transfections and cell stimulation\nThe generation, culture and activation of PKD1−/−, PKD3−/− and PKD1/3−/− knockout DT40 B cell lines have been described previously [1]. Cells were lysed and protein extracts were analysed in Western blotting experiments as previously described [1]. Chloramphenicol acetyl transferase assays have been described previously [29]."}