Id |
Subject |
Object |
Predicate |
Lexical cue |
T1725 |
0-10 |
NN |
denotes |
Generation |
T1726 |
11-13 |
IN |
denotes |
of |
T1727 |
14-20 |
NN |
denotes |
Trip13 |
T1728 |
28-32 |
NNS |
denotes |
Mice |
T1729 |
21-27 |
NN |
denotes |
Mutant |
T1730 |
32-182 |
sentence |
denotes |
To explore the function of TRIP13 in mammals, we generated mice with a gene trap-disrupted allele, Trip13RRB047 (Figure 1C; abbreviated as Trip13Gt). |
T1731 |
33-35 |
TO |
denotes |
To |
T1732 |
36-43 |
VB |
denotes |
explore |
T1733 |
82-91 |
VBD |
denotes |
generated |
T1734 |
44-47 |
DT |
denotes |
the |
T1735 |
48-56 |
NN |
denotes |
function |
T1736 |
57-59 |
IN |
denotes |
of |
T1737 |
60-66 |
NN |
denotes |
TRIP13 |
T1738 |
67-69 |
IN |
denotes |
in |
T1739 |
70-77 |
NNS |
denotes |
mammals |
T1740 |
77-79 |
, |
denotes |
, |
T1741 |
79-81 |
PRP |
denotes |
we |
T1742 |
92-96 |
NNS |
denotes |
mice |
T1743 |
97-101 |
IN |
denotes |
with |
T1744 |
102-103 |
DT |
denotes |
a |
T1745 |
124-130 |
NN |
denotes |
allele |
T1746 |
104-108 |
NN |
denotes |
gene |
T1747 |
109-113 |
NN |
denotes |
trap |
T1748 |
114-123 |
VBN |
denotes |
disrupted |
T1749 |
113-114 |
HYPH |
denotes |
- |
T1750 |
130-132 |
, |
denotes |
, |
T1751 |
132-144 |
NN |
denotes |
Trip13RRB047 |
T1752 |
145-146 |
-LRB- |
denotes |
( |
T1753 |
153-155 |
NN |
denotes |
1C |
T1754 |
146-152 |
NN |
denotes |
Figure |
T1755 |
155-156 |
: |
denotes |
; |
T1756 |
157-168 |
VBN |
denotes |
abbreviated |
T1757 |
169-171 |
IN |
denotes |
as |
T1758 |
172-180 |
NN |
denotes |
Trip13Gt |
T1759 |
180-181 |
-RRB- |
denotes |
) |
T1760 |
181-182 |
. |
denotes |
. |
T1761 |
182-374 |
sentence |
denotes |
Heterozygotes were normal in all respects, but homozygotes were present at ∼2/3 the expected ratio from intercrosses between heterozygotes (91 Trip13 +/+, 183 Trip13Gt/+, and 61 Trip13Gt/Gt). |
T1762 |
183-196 |
NNS |
denotes |
Heterozygotes |
T1763 |
197-201 |
VBD |
denotes |
were |
T1764 |
202-208 |
JJ |
denotes |
normal |
T1765 |
209-211 |
IN |
denotes |
in |
T1766 |
212-215 |
DT |
denotes |
all |
T1767 |
216-224 |
NNS |
denotes |
respects |
T1768 |
224-226 |
, |
denotes |
, |
T1769 |
226-229 |
CC |
denotes |
but |
T1770 |
230-241 |
NNS |
denotes |
homozygotes |
T1771 |
242-246 |
VBD |
denotes |
were |
T1772 |
247-254 |
JJ |
denotes |
present |
T1773 |
255-257 |
IN |
denotes |
at |
T1774 |
258-259 |
SYM |
denotes |
∼ |
T1775 |
261-262 |
CD |
denotes |
3 |
T1776 |
259-260 |
CD |
denotes |
2 |
T1777 |
260-261 |
SYM |
denotes |
/ |
T1778 |
276-281 |
NN |
denotes |
ratio |
T1779 |
263-266 |
DT |
denotes |
the |
T1780 |
267-275 |
VBN |
denotes |
expected |
T1781 |
282-286 |
IN |
denotes |
from |
T1782 |
287-299 |
NNS |
denotes |
intercrosses |
T1783 |
300-307 |
IN |
denotes |
between |
T1784 |
308-321 |
NNS |
denotes |
heterozygotes |
T1785 |
322-323 |
-LRB- |
denotes |
( |
T1786 |
323-325 |
CD |
denotes |
91 |
T1787 |
326-332 |
NN |
denotes |
Trip13 |
T1788 |
333-334 |
SYM |
denotes |
+ |
T1789 |
334-335 |
HYPH |
denotes |
/ |
T1790 |
335-336 |
SYM |
denotes |
+ |
T1791 |
336-338 |
, |
denotes |
, |
T1792 |
338-341 |
CD |
denotes |
183 |
T1793 |
342-350 |
NN |
denotes |
Trip13Gt |
T1794 |
350-351 |
HYPH |
denotes |
/ |
T1795 |
351-352 |
SYM |
denotes |
+ |
T1796 |
352-354 |
, |
denotes |
, |
T1797 |
354-357 |
CC |
denotes |
and |
T1798 |
358-360 |
CD |
denotes |
61 |
T1799 |
370-372 |
NN |
denotes |
Gt |
T1800 |
361-369 |
NN |
denotes |
Trip13Gt |
T1801 |
369-370 |
HYPH |
denotes |
/ |
T1802 |
372-373 |
-RRB- |
denotes |
) |
T1803 |
373-374 |
. |
denotes |
. |
T1804 |
374-507 |
sentence |
denotes |
Since >90% of prewean mice that died were mutant homozygotes, this discrepancy is apparently due to a partially penetrant lethality. |
T1805 |
375-380 |
IN |
denotes |
Since |
T1806 |
412-416 |
VBD |
denotes |
were |
T1807 |
381-382 |
SYM |
denotes |
> |
T1808 |
382-384 |
CD |
denotes |
90 |
T1809 |
384-385 |
NN |
denotes |
% |
T1810 |
386-388 |
IN |
denotes |
of |
T1811 |
389-396 |
JJ |
denotes |
prewean |
T1812 |
397-401 |
NNS |
denotes |
mice |
T1813 |
402-406 |
WDT |
denotes |
that |
T1814 |
407-411 |
VBD |
denotes |
died |
T1815 |
454-456 |
VBZ |
denotes |
is |
T1816 |
417-423 |
NN |
denotes |
mutant |
T1817 |
424-435 |
NNS |
denotes |
homozygotes |
T1818 |
435-437 |
, |
denotes |
, |
T1819 |
437-441 |
DT |
denotes |
this |
T1820 |
442-453 |
NN |
denotes |
discrepancy |
T1821 |
457-467 |
RB |
denotes |
apparently |
T1822 |
468-471 |
IN |
denotes |
due |
T1823 |
472-474 |
IN |
denotes |
to |
T1824 |
475-476 |
DT |
denotes |
a |
T1825 |
497-506 |
NN |
denotes |
lethality |
T1826 |
477-486 |
RB |
denotes |
partially |
T1827 |
487-496 |
JJ |
denotes |
penetrant |
T1828 |
506-507 |
. |
denotes |
. |
T1829 |
507-563 |
sentence |
denotes |
Most surviving Trip13Gt/Gt animals were grossly normal. |
T1830 |
508-512 |
JJS |
denotes |
Most |
T1831 |
535-542 |
NNS |
denotes |
animals |
T1832 |
513-522 |
VBG |
denotes |
surviving |
T1833 |
523-531 |
NN |
denotes |
Trip13Gt |
T1834 |
532-534 |
NN |
denotes |
Gt |
T1835 |
531-532 |
HYPH |
denotes |
/ |
T1836 |
543-547 |
VBD |
denotes |
were |
T1837 |
548-555 |
RB |
denotes |
grossly |
T1838 |
556-562 |
JJ |
denotes |
normal |
T1839 |
562-563 |
. |
denotes |
. |
T1840 |
563-719 |
sentence |
denotes |
However, homozygotes that were semi-congenic (N4) on the C57BL/6J strain were often markedly smaller and/or had kinked or shorter tails (Figure 2A and 2B). |
T1841 |
564-571 |
RB |
denotes |
However |
T1842 |
637-641 |
VBD |
denotes |
were |
T1843 |
571-573 |
, |
denotes |
, |
T1844 |
573-584 |
NNS |
denotes |
homozygotes |
T1845 |
585-589 |
WDT |
denotes |
that |
T1846 |
590-594 |
VBD |
denotes |
were |
T1878 |
1524-1527 |
NN |
denotes |
PCR |
T1879 |
1523-1524 |
HYPH |
denotes |
- |
T1880 |
1528-1536 |
NN |
denotes |
analysis |
T1881 |
1572-1580 |
VBD |
denotes |
revealed |
T1882 |
1537-1539 |
IN |
denotes |
of |
T1883 |
1540-1548 |
NN |
denotes |
Trip13Gt |
T1884 |
1549-1559 |
NN |
denotes |
expression |
T1885 |
1560-1561 |
-LRB- |
denotes |
( |
T1886 |
1568-1570 |
NN |
denotes |
1D |
T1887 |
1561-1567 |
NN |
denotes |
Figure |
T1888 |
1570-1571 |
-RRB- |
denotes |
) |
T1889 |
1581-1582 |
DT |
denotes |
a |
T1890 |
1587-1592 |
NN |
denotes |
level |
T1891 |
1583-1586 |
JJ |
denotes |
low |
T1892 |
1593-1595 |
IN |
denotes |
of |
T1893 |
1596-1604 |
RB |
denotes |
normally |
T1894 |
1605-1612 |
VBN |
denotes |
spliced |
T1895 |
1613-1624 |
NNS |
denotes |
transcripts |
T1896 |
1625-1627 |
IN |
denotes |
in |
T1897 |
1628-1634 |
NNS |
denotes |
testes |
T1898 |
1635-1637 |
IN |
denotes |
of |
T1899 |
1638-1649 |
NNS |
denotes |
homozygotes |
T1900 |
1650-1654 |
WDT |
denotes |
that |
T1901 |
1655-1657 |
VBZ |
denotes |
is |
T1902 |
1658-1668 |
RB |
denotes |
presumably |
T1903 |
1669-1670 |
DT |
denotes |
a |
T1904 |
1671-1682 |
NN |
denotes |
consequence |
T1905 |
1683-1685 |
IN |
denotes |
of |
T1906 |
1686-1696 |
JJ |
denotes |
incomplete |
T1907 |
1697-1702 |
NN |
denotes |
usage |
T1908 |
1703-1705 |
IN |
denotes |
of |
T1909 |
1706-1709 |
DT |
denotes |
the |
T1910 |
1715-1719 |
NN |
denotes |
trap |
T1911 |
1710-1714 |
NN |
denotes |
gene |
T1912 |
1729-1737 |
NN |
denotes |
acceptor |
T1913 |
1719-1721 |
POS |
denotes |
's |
T1914 |
1722-1728 |
NN |
denotes |
splice |
T1915 |
1737-1738 |
. |
denotes |
. |
T1916 |
1738-1966 |
sentence |
denotes |
Western blot analysis, using a polyclonal antibody raised against a peptide encoded by exon 3, revealed multiple species in wild-type and heterozygous testes, one of which corresponds to the expected size of 48 kDa (Figure 1E). |
T1917 |
1739-1746 |
NNP |
denotes |
Western |
T1918 |
1747-1751 |
NN |
denotes |
blot |
T1919 |
1752-1760 |
NN |
denotes |
analysis |
T1920 |
1834-1842 |
VBD |
denotes |
revealed |
T1921 |
1760-1762 |
, |
denotes |
, |
T1922 |
1762-1767 |
VBG |
denotes |
using |
T1923 |
1768-1769 |
DT |
denotes |
a |
T1924 |
1781-1789 |
NN |
denotes |
antibody |
T1925 |
1770-1780 |
JJ |
denotes |
polyclonal |
T1926 |
1790-1796 |
VBN |
denotes |
raised |
T1927 |
1797-1804 |
IN |
denotes |
against |
T1928 |
1805-1806 |
DT |
denotes |
a |
T1929 |
1807-1814 |
NN |
denotes |
peptide |
T1930 |
1815-1822 |
VBN |
denotes |
encoded |
T1931 |
1823-1825 |
IN |
denotes |
by |
T1932 |
1826-1830 |
NN |
denotes |
exon |
T1933 |
1831-1832 |
CD |
denotes |
3 |
T1934 |
1832-1834 |
, |
denotes |
, |
T1935 |
1843-1851 |
JJ |
denotes |
multiple |
T1936 |
1852-1859 |
NNS |
denotes |
species |
T1937 |
1860-1862 |
IN |
denotes |
in |
T1938 |
1863-1867 |
JJ |
denotes |
wild |
T1939 |
1868-1872 |
NN |
denotes |
type |
T1940 |
1867-1868 |
HYPH |
denotes |
- |
T1941 |
1890-1896 |
NNS |
denotes |
testes |
T1942 |
1873-1876 |
CC |
denotes |
and |
T1943 |
1877-1889 |
JJ |
denotes |
heterozygous |
T1944 |
1896-1898 |
, |
denotes |
, |
T1945 |
1898-1901 |
CD |
denotes |
one |
T1946 |
1911-1922 |
VBZ |
denotes |
corresponds |
T1947 |
1902-1904 |
IN |
denotes |
of |
T1948 |
1905-1910 |
WDT |
denotes |
which |
T1949 |
1923-1925 |
IN |
denotes |
to |
T1950 |
1926-1929 |
DT |
denotes |
the |
T1951 |
1939-1943 |
NN |
denotes |
size |
T1952 |
1930-1938 |
VBN |
denotes |
expected |
T1953 |
1944-1946 |
IN |
denotes |
of |
T1954 |
1947-1949 |
CD |
denotes |
48 |
T1955 |
1950-1953 |
NN |
denotes |
kDa |
T1956 |
1954-1955 |
-LRB- |
denotes |
( |
T1957 |
1962-1964 |
NN |
denotes |
1E |
T1958 |
1955-1961 |
NN |
denotes |
Figure |
T1959 |
1964-1965 |
-RRB- |
denotes |
) |
T1960 |
1965-1966 |
. |
denotes |
. |
T1961 |
1966-2111 |
sentence |
denotes |
This and three other species were undetectable in homozygous mutant testes, but a reduced amount of an intense ∼38 kDa smaller band was present. |
T1962 |
1967-1971 |
DT |
denotes |
This |
T1963 |
1996-2000 |
VBD |
denotes |
were |
T1964 |
1972-1975 |
CC |
denotes |
and |
T1965 |
1976-1981 |
CD |
denotes |
three |
T1966 |
1988-1995 |
NNS |
denotes |
species |
T1967 |
1982-1987 |
JJ |
denotes |
other |
T1968 |
2001-2013 |
JJ |
denotes |
undetectable |
T1969 |
2014-2016 |
IN |
denotes |
in |
T1970 |
2017-2027 |
JJ |
denotes |
homozygous |
T1971 |
2028-2034 |
NN |
denotes |
mutant |
T1972 |
2035-2041 |
NNS |
denotes |
testes |
T1973 |
2041-2043 |
, |
denotes |
, |
T1974 |
2043-2046 |
CC |
denotes |
but |
T1975 |
2047-2048 |
DT |
denotes |
a |
T1976 |
2057-2063 |
NN |
denotes |
amount |
T1977 |
2049-2056 |
VBN |
denotes |
reduced |
T1978 |
2099-2102 |
VBD |
denotes |
was |
T1979 |
2064-2066 |
IN |
denotes |
of |
T1980 |
2067-2069 |
DT |
denotes |
an |
T1981 |
2094-2098 |
NN |
denotes |
band |
T1982 |
2070-2077 |
JJ |
denotes |
intense |
T1983 |
2078-2079 |
SYM |
denotes |
∼ |
T1984 |
2079-2081 |
CD |
denotes |
38 |
T1985 |
2082-2085 |
NN |
denotes |
kDa |
T1986 |
2086-2093 |
JJR |
denotes |
smaller |
T1987 |
2103-2110 |
JJ |
denotes |
present |
T1988 |
2110-2111 |
. |
denotes |
. |
T1989 |
2111-2158 |
sentence |
denotes |
It is not clear if this corresponds to TRIP13. |
T1990 |
2112-2114 |
PRP |
denotes |
It |
T1991 |
2115-2117 |
VBZ |
denotes |
is |
T1992 |
2118-2121 |
RB |
denotes |
not |
T1993 |
2122-2127 |
JJ |
denotes |
clear |
T1994 |
2128-2130 |
IN |
denotes |
if |
T1995 |
2136-2147 |
VBZ |
denotes |
corresponds |
T1996 |
2131-2135 |
DT |
denotes |
this |
T1997 |
2148-2150 |
IN |
denotes |
to |
T1998 |
2151-2157 |
NN |
denotes |
TRIP13 |
T1999 |
2157-2158 |
. |
denotes |
. |
T2000 |
2158-2303 |
sentence |
denotes |
The greatly decreased Trip13 mRNA and predicted correct-length protein in mutants indicate that the Trip13RRB047 allele is severely hypomorphic. |
T2001 |
2159-2162 |
DT |
denotes |
The |
T2002 |
2188-2192 |
NN |
denotes |
mRNA |
T2003 |
2163-2170 |
RB |
denotes |
greatly |
T2004 |
2171-2180 |
VBN |
denotes |
decreased |
T2005 |
2181-2187 |
NN |
denotes |
Trip13 |
T2006 |
2241-2249 |
VBP |
denotes |
indicate |
T2007 |
2193-2196 |
CC |
denotes |
and |
T2008 |
2197-2206 |
VBN |
denotes |
predicted |
T2009 |
2222-2229 |
NN |
denotes |
protein |
T2010 |
2207-2214 |
JJ |
denotes |
correct |
T2011 |
2215-2221 |
NN |
denotes |
length |
T2012 |
2214-2215 |
HYPH |
denotes |
- |
T2013 |
2230-2232 |
IN |
denotes |
in |
T2014 |
2233-2240 |
NNS |
denotes |
mutants |
T2015 |
2250-2254 |
IN |
denotes |
that |
T2016 |
2279-2281 |
VBZ |
denotes |
is |
T2017 |
2255-2258 |
DT |
denotes |
the |
T2018 |
2272-2278 |
NN |
denotes |
allele |
T2019 |
2259-2271 |
NN |
denotes |
Trip13RRB047 |
T2020 |
2282-2290 |
RB |
denotes |
severely |
T2021 |
2291-2302 |
JJ |
denotes |
hypomorphic |
T2022 |
2302-2303 |
. |
denotes |
. |
T2023 |
2303-2575 |
sentence |
denotes |
To determine the germ cell types in which TRIP13 is expressed, and to assess possible expression in the mutant by means other than Western analysis, testis sections were immunolabeled for TRIP13 using a polyclonal chicken antipeptide antibody (see Materials and Methods). |
T2024 |
2304-2306 |
TO |
denotes |
To |
T2025 |
2307-2316 |
VB |
denotes |
determine |
T2026 |
2474-2487 |
VBN |
denotes |
immunolabeled |
T2027 |
2317-2320 |
DT |
denotes |
the |
T2028 |
2331-2336 |
NNS |
denotes |
types |
T2029 |
2321-2325 |
NN |
denotes |
germ |
T2030 |
2326-2330 |
NN |
denotes |
cell |
T2031 |
2337-2339 |
IN |
denotes |
in |
T2032 |
2356-2365 |
VBN |
denotes |
expressed |
T2033 |
2340-2345 |
WDT |
denotes |
which |
T2034 |
2346-2352 |
NN |
denotes |
TRIP13 |
T2035 |
2353-2355 |
VBZ |
denotes |
is |
T2036 |
2365-2367 |
, |
denotes |
, |
T2037 |
2367-2370 |
CC |
denotes |
and |
T2038 |
2371-2373 |
TO |
denotes |
to |
T2039 |
2374-2380 |
VB |
denotes |
assess |
T2040 |
2381-2389 |
JJ |
denotes |
possible |
T2041 |
2390-2400 |
NN |
denotes |
expression |
T2042 |
2401-2403 |
IN |
denotes |
in |
T2043 |
2404-2407 |
DT |
denotes |
the |
T2044 |
2408-2414 |
NN |
denotes |
mutant |
T2045 |
2415-2417 |
IN |
denotes |
by |
T2046 |
2418-2423 |
NNS |
denotes |
means |
T2047 |
2424-2429 |
JJ |
denotes |
other |
T2048 |
2430-2434 |
IN |
denotes |
than |
T2049 |
2435-2442 |
NNP |
denotes |
Western |
T2050 |
2443-2451 |
NN |
denotes |
analysis |
T2051 |
2451-2453 |
, |
denotes |
, |
T2052 |
2453-2459 |
NN |
denotes |
testis |
T2053 |
2460-2468 |
NNS |
denotes |
sections |
T2054 |
2469-2473 |
VBD |
denotes |
were |
T2055 |
2488-2491 |
IN |
denotes |
for |
T2056 |
2492-2498 |
NN |
denotes |
TRIP13 |
T2057 |
2499-2504 |
VBG |
denotes |
using |
T2058 |
2505-2506 |
DT |
denotes |
a |
T2059 |
2538-2546 |
NN |
denotes |
antibody |
T2060 |
2507-2517 |
JJ |
denotes |
polyclonal |
T2061 |
2526-2537 |
NN |
denotes |
antipeptide |
T2062 |
2518-2525 |
NN |
denotes |
chicken |
T2063 |
2547-2548 |
-LRB- |
denotes |
( |
T2064 |
2548-2551 |
VB |
denotes |
see |
T2065 |
2552-2561 |
NNS |
denotes |
Materials |
T2066 |
2562-2565 |
CC |
denotes |
and |
T2067 |
2566-2573 |
NNS |
denotes |
Methods |
T2068 |
2573-2574 |
-RRB- |
denotes |
) |
T2069 |
2574-2575 |
. |
denotes |
. |
T2070 |
2575-2693 |
sentence |
denotes |
The most intensely labeled cells in control testes were Type B spermatogonia and leptotene spermatocytes (Figure 1F). |
T2071 |
2576-2579 |
DT |
denotes |
The |
T2072 |
2603-2608 |
NNS |
denotes |
cells |
T2073 |
2580-2584 |
RBS |
denotes |
most |
T2074 |
2585-2594 |
RB |
denotes |
intensely |
T2075 |
2595-2602 |
VBN |
denotes |
labeled |
T2076 |
2627-2631 |
VBD |
denotes |
were |
T2077 |
2609-2611 |
IN |
denotes |
in |
T2078 |
2612-2619 |
NN |
denotes |
control |
T2079 |
2620-2626 |
NNS |
denotes |
testes |
T2080 |
2632-2636 |
NN |
denotes |
Type |
T2081 |
2637-2638 |
NN |
denotes |
B |
T2082 |
2639-2652 |
NNS |
denotes |
spermatogonia |
T2083 |
2653-2656 |
CC |
denotes |
and |
T2084 |
2657-2666 |
NN |
denotes |
leptotene |
T2085 |
2667-2680 |
NNS |
denotes |
spermatocytes |
T2086 |
2681-2682 |
-LRB- |
denotes |
( |
T2087 |
2689-2691 |
NN |
denotes |
1F |
T2088 |
2682-2688 |
NN |
denotes |
Figure |
T2089 |
2691-2692 |
-RRB- |
denotes |
) |
T2090 |
2692-2693 |
. |
denotes |
. |
T2091 |
2693-2819 |
sentence |
denotes |
Zygotene/pachytene spermatocytes stained less strongly, and there was no detectable staining in late pachytene spermatocytes. |
T2092 |
2694-2702 |
NN |
denotes |
Zygotene |
T2093 |
2703-2712 |
NN |
denotes |
pachytene |
T2094 |
2702-2703 |
HYPH |
denotes |
/ |
T2095 |
2713-2726 |
NNS |
denotes |
spermatocytes |
T2096 |
2727-2734 |
VBD |
denotes |
stained |
T2097 |
2735-2739 |
RBR |
denotes |
less |
T2098 |
2740-2748 |
RB |
denotes |
strongly |
T2099 |
2748-2750 |
, |
denotes |
, |
T2100 |
2750-2753 |
CC |
denotes |
and |
T2101 |
2754-2759 |
EX |
denotes |
there |
T2102 |
2760-2763 |
VBD |
denotes |
was |
T2103 |
2764-2766 |
DT |
denotes |
no |
T2104 |
2778-2786 |
NN |
denotes |
staining |
T2105 |
2767-2777 |
JJ |
denotes |
detectable |
T2106 |
2787-2789 |
IN |
denotes |
in |
T2107 |
2790-2794 |
JJ |
denotes |
late |
T2108 |
2795-2804 |
NN |
denotes |
pachytene |
T2109 |
2805-2818 |
NNS |
denotes |
spermatocytes |
T2110 |
2818-2819 |
. |
denotes |
. |
T2111 |
2819-2860 |
sentence |
denotes |
TRIP13 appeared to be nuclear localized. |
T2112 |
2820-2826 |
NN |
denotes |
TRIP13 |
T2113 |
2827-2835 |
VBD |
denotes |
appeared |
T2114 |
2836-2838 |
TO |
denotes |
to |
T2115 |
2839-2841 |
VB |
denotes |
be |
T2116 |
2842-2849 |
JJ |
denotes |
nuclear |
T2117 |
2850-2859 |
VBN |
denotes |
localized |
T2118 |
2859-2860 |
. |
denotes |
. |
T2119 |
2860-2941 |
sentence |
denotes |
There was no such staining of nuclei in mutant seminiferous tubules (Figure 1F). |
T2120 |
2861-2866 |
EX |
denotes |
There |
T2121 |
2867-2870 |
VBD |
denotes |
was |
T2122 |
2871-2873 |
DT |
denotes |
no |
T2123 |
2879-2887 |
NN |
denotes |
staining |
T2124 |
2874-2878 |
JJ |
denotes |
such |
T2125 |
2888-2890 |
IN |
denotes |
of |
T2126 |
2891-2897 |
NNS |
denotes |
nuclei |
T2127 |
2898-2900 |
IN |
denotes |
in |
T2128 |
2901-2907 |
NN |
denotes |
mutant |
T2129 |
2921-2928 |
NNS |
denotes |
tubules |
T2130 |
2908-2920 |
JJ |
denotes |
seminiferous |
T2131 |
2929-2930 |
-LRB- |
denotes |
( |
T2132 |
2937-2939 |
NN |
denotes |
1F |
T2133 |
2930-2936 |
NN |
denotes |
Figure |
T2134 |
2939-2940 |
-RRB- |
denotes |
) |
T2135 |
2940-2941 |
. |
denotes |
. |
T2136 |
2941-3084 |
sentence |
denotes |
To further assess the nuclear localization, TRIP13 was used to probe meiotic chromosomes prepared by surface spreading of spermatocyte nuclei. |
T2137 |
2942-2944 |
TO |
denotes |
To |
T2138 |
2953-2959 |
VB |
denotes |
assess |
T2139 |
2945-2952 |
RB |
denotes |
further |
T2140 |
2997-3001 |
VBN |
denotes |
used |
T2141 |
2960-2963 |
DT |
denotes |
the |
T2142 |
2972-2984 |
NN |
denotes |
localization |
T2143 |
2964-2971 |
JJ |
denotes |
nuclear |
T2144 |
2984-2986 |
, |
denotes |
, |
T2145 |
2986-2992 |
NN |
denotes |
TRIP13 |
T2146 |
2993-2996 |
VBD |
denotes |
was |
T2147 |
3002-3004 |
TO |
denotes |
to |
T2148 |
3005-3010 |
VB |
denotes |
probe |
T2149 |
3011-3018 |
JJ |
denotes |
meiotic |
T2150 |
3019-3030 |
NNS |
denotes |
chromosomes |
T2151 |
3031-3039 |
VBN |
denotes |
prepared |
T2152 |
3040-3042 |
IN |
denotes |
by |
T2153 |
3043-3050 |
NN |
denotes |
surface |
T2154 |
3051-3060 |
NN |
denotes |
spreading |
T2155 |
3061-3063 |
IN |
denotes |
of |
T2156 |
3064-3076 |
NN |
denotes |
spermatocyte |
T2157 |
3077-3083 |
NNS |
denotes |
nuclei |
T2158 |
3083-3084 |
. |
denotes |
. |
T2159 |
3084-3260 |
sentence |
denotes |
In wild type, there was diffuse nuclear staining, and no evidence of concentration on SC cores (marked by the axial element protein SYCP3) at any meiotic substage (Figure 1G). |
T2160 |
3085-3087 |
IN |
denotes |
In |
T2161 |
3105-3108 |
VBD |
denotes |
was |
T2162 |
3088-3092 |
JJ |
denotes |
wild |
T2163 |
3093-3097 |
NN |
denotes |
type |
T2164 |
3097-3099 |
, |
denotes |
, |
T2165 |
3099-3104 |
EX |
denotes |
there |
T2166 |
3109-3116 |
JJ |
denotes |
diffuse |
T2167 |
3125-3133 |
NN |
denotes |
staining |
T2168 |
3117-3124 |
JJ |
denotes |
nuclear |
T2169 |
3133-3135 |
, |
denotes |
, |
T2170 |
3135-3138 |
CC |
denotes |
and |
T2171 |
3139-3141 |
DT |
denotes |
no |
T2172 |
3142-3150 |
NN |
denotes |
evidence |
T2173 |
3151-3153 |
IN |
denotes |
of |
T2174 |
3154-3167 |
NN |
denotes |
concentration |
T2175 |
3168-3170 |
IN |
denotes |
on |
T2176 |
3171-3173 |
NN |
denotes |
SC |
T2177 |
3174-3179 |
NNS |
denotes |
cores |
T2178 |
3180-3181 |
-LRB- |
denotes |
( |
T2179 |
3181-3187 |
VBN |
denotes |
marked |
T2180 |
3188-3190 |
IN |
denotes |
by |
T2181 |
3191-3194 |
DT |
denotes |
the |
T2182 |
3217-3222 |
NN |
denotes |
SYCP3 |
T2183 |
3195-3200 |
JJ |
denotes |
axial |
T2184 |
3201-3208 |
NN |
denotes |
element |
T2185 |
3209-3216 |
NN |
denotes |
protein |
T2186 |
3222-3223 |
-RRB- |
denotes |
) |
T2187 |
3224-3226 |
IN |
denotes |
at |
T2188 |
3227-3230 |
DT |
denotes |
any |
T2189 |
3239-3247 |
NN |
denotes |
substage |
T2190 |
3231-3238 |
JJ |
denotes |
meiotic |
T2191 |
3248-3249 |
-LRB- |
denotes |
( |
T2192 |
3256-3258 |
NN |
denotes |
1G |
T2193 |
3249-3255 |
NN |
denotes |
Figure |
T2194 |
3258-3259 |
-RRB- |
denotes |
) |
T2195 |
3259-3260 |
. |
denotes |
. |
T2196 |
3260-3322 |
sentence |
denotes |
TRIP13 signal was noticeably absent in mutant meiotic nuclei. |
T2197 |
3261-3267 |
NN |
denotes |
TRIP13 |
T2198 |
3268-3274 |
NN |
denotes |
signal |
T2199 |
3275-3278 |
VBD |
denotes |
was |
T2200 |
3279-3289 |
RB |
denotes |
noticeably |
T2201 |
3290-3296 |
JJ |
denotes |
absent |
T2202 |
3297-3299 |
IN |
denotes |
in |
T2203 |
3300-3306 |
NN |
denotes |
mutant |
T2204 |
3315-3321 |
NNS |
denotes |
nuclei |
T2205 |
3307-3314 |
JJ |
denotes |
meiotic |
T2206 |
3321-3322 |
. |
denotes |
. |
T10698 |
730-743 |
JJ |
denotes |
Developmental |
T10699 |
744-754 |
NNS |
denotes |
Phenotypes |
T10700 |
755-757 |
IN |
denotes |
of |
T10701 |
758-764 |
NN |
denotes |
Trip13 |
T10702 |
765-771 |
NN |
denotes |
Mutant |
T10703 |
772-776 |
NNS |
denotes |
Mice |
T10704 |
776-812 |
sentence |
denotes |
(A) Shown are 21-d-old littermates. |
T10705 |
777-778 |
-LRB- |
denotes |
( |
T10706 |
778-779 |
LS |
denotes |
A |
T10707 |
787-790 |
VBP |
denotes |
are |
T10708 |
779-780 |
-RRB- |
denotes |
) |
T10709 |
781-786 |
VBN |
denotes |
Shown |
T10710 |
791-793 |
CD |
denotes |
21 |
T10711 |
794-795 |
NN |
denotes |
d |
T10712 |
793-794 |
HYPH |
denotes |
- |
T10713 |
796-799 |
JJ |
denotes |
old |
T10714 |
795-796 |
HYPH |
denotes |
- |
T10715 |
800-811 |
NNS |
denotes |
littermates |
T10716 |
811-812 |
. |
denotes |
. |
T10717 |
812-882 |
sentence |
denotes |
Note the shortened tail in the mutant, but overall similar body size. |
T10718 |
813-817 |
VB |
denotes |
Note |
T10719 |
818-821 |
DT |
denotes |
the |
T10720 |
832-836 |
NN |
denotes |
tail |
T10721 |
822-831 |
VBN |
denotes |
shortened |
T10722 |
837-839 |
IN |
denotes |
in |
T10723 |
840-843 |
DT |
denotes |
the |
T10724 |
844-850 |
NN |
denotes |
mutant |
T10725 |
850-852 |
, |
denotes |
, |
T10726 |
852-855 |
CC |
denotes |
but |
T10727 |
856-863 |
JJ |
denotes |
overall |
T10728 |
864-871 |
JJ |
denotes |
similar |
T10729 |
877-881 |
NN |
denotes |
size |
T10730 |
872-876 |
NN |
denotes |
body |
T10731 |
881-882 |
. |
denotes |
. |
T10732 |
882-918 |
sentence |
denotes |
(B) Shown are 23-d-old littermates. |
T10733 |
883-884 |
-LRB- |
denotes |
( |
T10734 |
884-885 |
LS |
denotes |
B |
T10735 |
893-896 |
VBP |
denotes |
are |
T10736 |
885-886 |
-RRB- |
denotes |
) |
T10737 |
887-892 |
VBN |
denotes |
Shown |
T10738 |
897-899 |
CD |
denotes |
23 |
T10739 |
900-901 |
NN |
denotes |
d |
T10740 |
899-900 |
HYPH |
denotes |
- |
T10741 |
902-905 |
JJ |
denotes |
old |
T10742 |
901-902 |
HYPH |
denotes |
- |
T10743 |
906-917 |
NNS |
denotes |
littermates |
T10744 |
917-918 |
. |
denotes |
. |
T10745 |
918-1008 |
sentence |
denotes |
The mutant is smaller in this case, but the tail is not as truncated as the mouse in (A). |
T10746 |
919-922 |
DT |
denotes |
The |
T10747 |
923-929 |
NN |
denotes |
mutant |
T10748 |
930-932 |
VBZ |
denotes |
is |
T10749 |
933-940 |
JJR |
denotes |
smaller |
T10750 |
941-943 |
IN |
denotes |
in |
T10751 |
944-948 |
DT |
denotes |
this |
T10752 |
949-953 |
NN |
denotes |
case |
T10753 |
953-955 |
, |
denotes |
, |
T10754 |
955-958 |
CC |
denotes |
but |
T10755 |
959-962 |
DT |
denotes |
the |
T10756 |
963-967 |
NN |
denotes |
tail |
T10757 |
968-970 |
VBZ |
denotes |
is |
T10758 |
971-974 |
RB |
denotes |
not |
T10759 |
975-977 |
RB |
denotes |
as |
T10760 |
978-987 |
JJ |
denotes |
truncated |
T10761 |
988-990 |
IN |
denotes |
as |
T10762 |
991-994 |
DT |
denotes |
the |
T10763 |
995-1000 |
NN |
denotes |
mouse |
T10764 |
1001-1003 |
IN |
denotes |
in |
T10765 |
1004-1005 |
-LRB- |
denotes |
( |
T10766 |
1005-1006 |
NN |
denotes |
A |
T10767 |
1006-1007 |
-RRB- |
denotes |
) |
T10768 |
1007-1008 |
. |
denotes |
. |
T10769 |
1008-1070 |
sentence |
denotes |
(C) Wild-type (WT) and homozygous Trip13 mutant (MUT) testes. |
T10770 |
1009-1010 |
-LRB- |
denotes |
( |
T10771 |
1010-1011 |
LS |
denotes |
C |
T10772 |
1063-1069 |
NNS |
denotes |
testes |
T10773 |
1011-1012 |
-RRB- |
denotes |
) |
T10774 |
1013-1017 |
JJ |
denotes |
Wild |
T10775 |
1018-1022 |
NN |
denotes |
type |
T10776 |
1017-1018 |
HYPH |
denotes |
- |
T10777 |
1023-1024 |
-LRB- |
denotes |
( |
T10778 |
1024-1026 |
NN |
denotes |
WT |
T10779 |
1026-1027 |
-RRB- |
denotes |
) |
T10780 |
1028-1031 |
CC |
denotes |
and |
T10781 |
1032-1042 |
JJ |
denotes |
homozygous |
T10782 |
1050-1056 |
NN |
denotes |
mutant |
T10783 |
1043-1049 |
NN |
denotes |
Trip13 |
T10784 |
1057-1058 |
-LRB- |
denotes |
( |
T10785 |
1058-1061 |
NN |
denotes |
MUT |
T10786 |
1061-1062 |
-RRB- |
denotes |
) |
T10787 |
1069-1070 |
. |
denotes |
. |
T10788 |
1070-1191 |
sentence |
denotes |
(D) and (E) are cross sections through 17.5-d-old heterozygous (“WT”) and homozygous mutant Trip13 testes, respectively. |
T10789 |
1071-1072 |
-LRB- |
denotes |
( |
T10790 |
1072-1073 |
NN |
denotes |
D |
T10791 |
1083-1086 |
VBP |
denotes |
are |
T10792 |
1073-1074 |
-RRB- |
denotes |
) |
T10793 |
1075-1078 |
CC |
denotes |
and |
T10794 |
1079-1080 |
-LRB- |
denotes |
( |
T10795 |
1080-1081 |
NN |
denotes |
E |
T10796 |
1081-1082 |
-RRB- |
denotes |
) |
T10797 |
1087-1092 |
NN |
denotes |
cross |
T10798 |
1093-1101 |
NNS |
denotes |
sections |
T10799 |
1102-1109 |
IN |
denotes |
through |
T10800 |
1110-1114 |
CD |
denotes |
17.5 |
T10801 |
1115-1116 |
NN |
denotes |
d |
T10802 |
1114-1115 |
HYPH |
denotes |
- |
T10803 |
1117-1120 |
JJ |
denotes |
old |
T10804 |
1116-1117 |
HYPH |
denotes |
- |
T10805 |
1170-1176 |
NNS |
denotes |
testes |
T10806 |
1121-1133 |
JJ |
denotes |
heterozygous |
T10807 |
1156-1162 |
NN |
denotes |
mutant |
T10808 |
1134-1135 |
-LRB- |
denotes |
( |
T10809 |
1136-1138 |
NN |
denotes |
WT |
T10810 |
1135-1136 |
`` |
denotes |
“ |
T10811 |
1138-1139 |
'' |
denotes |
” |
T10812 |
1139-1140 |
-RRB- |
denotes |
) |
T10813 |
1141-1144 |
CC |
denotes |
and |
T10814 |
1145-1155 |
JJ |
denotes |
homozygous |
T10815 |
1163-1169 |
NN |
denotes |
Trip13 |
T10816 |
1176-1178 |
, |
denotes |
, |
T10817 |
1178-1190 |
RB |
denotes |
respectively |
T10818 |
1190-1191 |
. |
denotes |
. |
T10819 |
1191-1404 |
sentence |
denotes |
Whereas the tubules in WT show coordinated spermatogenesis with pachytene spermatocytes present in all tubules (proximal to the lumen), developmental progression in the mutant is not synchronized between tubules. |
T10820 |
1192-1199 |
IN |
denotes |
Whereas |
T10821 |
1218-1222 |
VBP |
denotes |
show |
T10822 |
1200-1203 |
DT |
denotes |
the |
T10823 |
1204-1211 |
NNS |
denotes |
tubules |
T10824 |
1212-1214 |
IN |
denotes |
in |
T10825 |
1215-1217 |
NN |
denotes |
WT |
T10826 |
1375-1387 |
VBN |
denotes |
synchronized |
T10827 |
1223-1234 |
VBN |
denotes |
coordinated |
T10828 |
1235-1250 |
NN |
denotes |
spermatogenesis |
T10829 |
1251-1255 |
IN |
denotes |
with |
T10830 |
1256-1265 |
NN |
denotes |
pachytene |
T10831 |
1266-1279 |
NNS |
denotes |
spermatocytes |
T10832 |
1280-1287 |
JJ |
denotes |
present |
T10833 |
1288-1290 |
IN |
denotes |
in |
T10834 |
1291-1294 |
DT |
denotes |
all |
T10835 |
1295-1302 |
NNS |
denotes |
tubules |
T10836 |
1303-1304 |
-LRB- |
denotes |
( |
T10837 |
1304-1312 |
JJ |
denotes |
proximal |
T10838 |
1313-1315 |
IN |
denotes |
to |
T10839 |
1316-1319 |
DT |
denotes |
the |
T10840 |
1320-1325 |
NN |
denotes |
lumen |
T10841 |
1325-1326 |
-RRB- |
denotes |
) |
T10842 |
1326-1328 |
, |
denotes |
, |
T10843 |
1328-1341 |
JJ |
denotes |
developmental |
T10844 |
1342-1353 |
NN |
denotes |
progression |
T10845 |
1354-1356 |
IN |
denotes |
in |
T10846 |
1357-1360 |
DT |
denotes |
the |
T10847 |
1361-1367 |
NN |
denotes |
mutant |
T10848 |
1368-1370 |
VBZ |
denotes |
is |
T10849 |
1371-1374 |
RB |
denotes |
not |
T10850 |
1388-1395 |
IN |
denotes |
between |
T10851 |
1396-1403 |
NNS |
denotes |
tubules |
T10852 |
1403-1404 |
. |
denotes |
. |
T10853 |
1404-1520 |
sentence |
denotes |
Some tubules have no pachytene spermatocyes (asterisks), while in others, development is somewhat disorganized (#). |
T10854 |
1405-1409 |
DT |
denotes |
Some |
T10855 |
1410-1417 |
NNS |
denotes |
tubules |
T10856 |
1418-1422 |
VBP |
denotes |
have |
T10857 |
1423-1425 |
DT |
denotes |
no |
T10858 |
1436-1448 |
NNS |
denotes |
spermatocyes |
T10859 |
1426-1435 |
NN |
denotes |
pachytene |
T10860 |
1449-1450 |
-LRB- |
denotes |
( |
T10861 |
1450-1459 |
NNS |
denotes |
asterisks |
T10862 |
1459-1460 |
-RRB- |
denotes |
) |
T10863 |
1460-1462 |
, |
denotes |
, |
T10864 |
1462-1467 |
IN |
denotes |
while |
T10865 |
1491-1493 |
VBZ |
denotes |
is |
T10866 |
1468-1470 |
IN |
denotes |
in |
T10867 |
1471-1477 |
NNS |
denotes |
others |
T10868 |
1477-1479 |
, |
denotes |
, |
T10869 |
1479-1490 |
NN |
denotes |
development |
T10870 |
1494-1502 |
RB |
denotes |
somewhat |
T10871 |
1503-1515 |
JJ |
denotes |
disorganized |
T10872 |
1516-1517 |
-LRB- |
denotes |
( |
T10873 |
1517-1518 |
SYM |
denotes |
# |
T10874 |
1518-1519 |
-RRB- |
denotes |
) |
T10875 |
1519-1520 |
. |
denotes |
. |
T1847 |
595-608 |
JJ |
denotes |
semi-congenic |
T1848 |
609-610 |
-LRB- |
denotes |
( |
T1849 |
610-612 |
NN |
denotes |
N4 |
T1850 |
612-613 |
-RRB- |
denotes |
) |
T1851 |
614-616 |
IN |
denotes |
on |
T1852 |
617-620 |
DT |
denotes |
the |
T1853 |
630-636 |
NN |
denotes |
strain |
T1854 |
621-626 |
NN |
denotes |
C57BL |
T1855 |
627-629 |
NN |
denotes |
6J |
T1856 |
626-627 |
HYPH |
denotes |
/ |
T1857 |
642-647 |
RB |
denotes |
often |
T1858 |
648-656 |
RB |
denotes |
markedly |
T1859 |
657-664 |
JJR |
denotes |
smaller |
T1860 |
665-668 |
CC |
denotes |
and |
T1861 |
668-669 |
HYPH |
denotes |
/ |
T1862 |
669-671 |
CC |
denotes |
or |
T1863 |
672-675 |
VBD |
denotes |
had |
T1864 |
676-682 |
VBN |
denotes |
kinked |
T1865 |
694-699 |
NNS |
denotes |
tails |
T1866 |
683-685 |
CC |
denotes |
or |
T1867 |
686-693 |
JJR |
denotes |
shorter |
T1868 |
700-701 |
-LRB- |
denotes |
( |
T1869 |
708-710 |
NN |
denotes |
2A |
T1870 |
701-707 |
NN |
denotes |
Figure |
T1871 |
711-714 |
CC |
denotes |
and |
T1872 |
715-717 |
NN |
denotes |
2B |
T1873 |
717-718 |
-RRB- |
denotes |
) |
T1874 |
718-719 |
. |
denotes |
. |
T1875 |
719-720 |
sentence |
denotes |
|
T1876 |
1521-1738 |
sentence |
denotes |
RT-PCR analysis of Trip13Gt expression (Figure 1D) revealed a low level of normally spliced transcripts in testes of homozygotes that is presumably a consequence of incomplete usage of the gene trap's splice acceptor. |
T1877 |
1521-1523 |
NN |
denotes |
RT |
R1028 |
T1726 |
T1725 |
prep |
of,Generation |
R1029 |
T1727 |
T1728 |
compound |
Trip13,Mice |
R1030 |
T1728 |
T1726 |
pobj |
Mice,of |
R1031 |
T1729 |
T1728 |
compound |
Mutant,Mice |
R1032 |
T1731 |
T1732 |
aux |
To,explore |
R1033 |
T1732 |
T1733 |
advcl |
explore,generated |
R1034 |
T1734 |
T1735 |
det |
the,function |
R1035 |
T1735 |
T1732 |
dobj |
function,explore |
R1036 |
T1736 |
T1735 |
prep |
of,function |
R1037 |
T1737 |
T1736 |
pobj |
TRIP13,of |
R1038 |
T1738 |
T1735 |
prep |
in,function |
R1039 |
T1739 |
T1738 |
pobj |
mammals,in |
R1040 |
T1740 |
T1733 |
punct |
", ",generated |
R1041 |
T1741 |
T1733 |
nsubj |
we,generated |
R1042 |
T1742 |
T1733 |
dobj |
mice,generated |
R1043 |
T1743 |
T1742 |
prep |
with,mice |
R1044 |
T1744 |
T1745 |
det |
a,allele |
R1045 |
T1745 |
T1743 |
pobj |
allele,with |
R1046 |
T1746 |
T1747 |
compound |
gene,trap |
R1047 |
T1747 |
T1748 |
npadvmod |
trap,disrupted |
R1048 |
T1748 |
T1745 |
amod |
disrupted,allele |
R1049 |
T1749 |
T1748 |
punct |
-,disrupted |
R1050 |
T1750 |
T1745 |
punct |
", ",allele |
R1051 |
T1751 |
T1745 |
appos |
Trip13RRB047,allele |
R1052 |
T1752 |
T1753 |
punct |
(,1C |
R1053 |
T1753 |
T1733 |
parataxis |
1C,generated |
R1054 |
T1754 |
T1753 |
compound |
Figure,1C |
R1055 |
T1755 |
T1753 |
punct |
;,1C |
R1056 |
T1756 |
T1753 |
advcl |
abbreviated,1C |
R1057 |
T1757 |
T1756 |
prep |
as,abbreviated |
R1058 |
T1758 |
T1757 |
pobj |
Trip13Gt,as |
R1059 |
T1759 |
T1753 |
punct |
),1C |
R1060 |
T1760 |
T1733 |
punct |
.,generated |
R1061 |
T1762 |
T1763 |
nsubj |
Heterozygotes,were |
R1062 |
T1764 |
T1763 |
acomp |
normal,were |
R1063 |
T1765 |
T1763 |
prep |
in,were |
R1064 |
T1766 |
T1767 |
det |
all,respects |
R1065 |
T1767 |
T1765 |
pobj |
respects,in |
R1066 |
T1768 |
T1763 |
punct |
", ",were |
R1067 |
T1769 |
T1763 |
cc |
but,were |
R1068 |
T1770 |
T1771 |
nsubj |
homozygotes,were |
R1069 |
T1771 |
T1763 |
conj |
were,were |
R1070 |
T1772 |
T1771 |
acomp |
present,were |
R1071 |
T1773 |
T1771 |
prep |
at,were |
R1072 |
T1774 |
T1775 |
punct |
∼,3 |
R1073 |
T1775 |
T1778 |
nummod |
3,ratio |
R1074 |
T1776 |
T1775 |
quantmod |
2,3 |
R1075 |
T1777 |
T1775 |
punct |
/,3 |
R1076 |
T1778 |
T1773 |
pobj |
ratio,at |
R1077 |
T1779 |
T1778 |
det |
the,ratio |
R1078 |
T1780 |
T1778 |
amod |
expected,ratio |
R1079 |
T1781 |
T1778 |
prep |
from,ratio |
R1080 |
T1782 |
T1781 |
pobj |
intercrosses,from |
R1081 |
T1783 |
T1782 |
prep |
between,intercrosses |
R1082 |
T1784 |
T1783 |
pobj |
heterozygotes,between |
R1083 |
T1785 |
T1782 |
punct |
(,intercrosses |
R1084 |
T1786 |
T1787 |
nummod |
91,Trip13 |
R1085 |
T1787 |
T1782 |
appos |
Trip13,intercrosses |
R1086 |
T1788 |
T1787 |
punct |
+,Trip13 |
R1087 |
T1789 |
T1787 |
punct |
/,Trip13 |
R1088 |
T1790 |
T1787 |
punct |
+,Trip13 |
R1089 |
T1791 |
T1787 |
punct |
", ",Trip13 |
R1090 |
T1792 |
T1793 |
nummod |
183,Trip13Gt |
R1091 |
T1793 |
T1787 |
conj |
Trip13Gt,Trip13 |
R1092 |
T1794 |
T1793 |
punct |
/,Trip13Gt |
R1093 |
T1795 |
T1793 |
punct |
+,Trip13Gt |
R1094 |
T1796 |
T1793 |
punct |
", ",Trip13Gt |
R1095 |
T1797 |
T1793 |
cc |
and,Trip13Gt |
R1096 |
T1798 |
T1799 |
nummod |
61,Gt |
R1097 |
T1799 |
T1793 |
conj |
Gt,Trip13Gt |
R1098 |
T1800 |
T1799 |
compound |
Trip13Gt,Gt |
R1099 |
T1801 |
T1799 |
punct |
/,Gt |
R1100 |
T1802 |
T1771 |
punct |
),were |
R1101 |
T1803 |
T1771 |
punct |
.,were |
R1102 |
T1805 |
T1806 |
mark |
Since,were |
R1103 |
T1806 |
T1815 |
advcl |
were,is |
R1104 |
T1807 |
T1808 |
punct |
>,90 |
R1105 |
T1808 |
T1809 |
nummod |
90,% |
R1106 |
T1809 |
T1806 |
nsubj |
%,were |
R1107 |
T1810 |
T1809 |
prep |
of,% |
R1108 |
T1811 |
T1812 |
amod |
prewean,mice |
R1109 |
T1812 |
T1810 |
pobj |
mice,of |
R1110 |
T1813 |
T1814 |
dep |
that,died |
R1111 |
T1814 |
T1812 |
relcl |
died,mice |
R1112 |
T1816 |
T1817 |
compound |
mutant,homozygotes |
R1113 |
T1817 |
T1806 |
attr |
homozygotes,were |
R1114 |
T1818 |
T1815 |
punct |
", ",is |
R1115 |
T1819 |
T1820 |
det |
this,discrepancy |
R1116 |
T1820 |
T1815 |
nsubj |
discrepancy,is |
R1117 |
T1821 |
T1815 |
advmod |
apparently,is |
R1118 |
T1822 |
T1815 |
prep |
due,is |
R1119 |
T1823 |
T1822 |
pcomp |
to,due |
R1120 |
T1824 |
T1825 |
det |
a,lethality |
R1121 |
T1825 |
T1822 |
pobj |
lethality,due |
R1122 |
T1826 |
T1827 |
advmod |
partially,penetrant |
R1123 |
T1827 |
T1825 |
amod |
penetrant,lethality |
R1124 |
T1828 |
T1815 |
punct |
.,is |
R1125 |
T1830 |
T1831 |
amod |
Most,animals |
R1126 |
T1831 |
T1836 |
nsubj |
animals,were |
R1127 |
T1832 |
T1831 |
amod |
surviving,animals |
R1128 |
T1833 |
T1834 |
compound |
Trip13Gt,Gt |
R1129 |
T1834 |
T1831 |
compound |
Gt,animals |
R1130 |
T1835 |
T1834 |
punct |
/,Gt |
R1131 |
T1837 |
T1838 |
advmod |
grossly,normal |
R1132 |
T1838 |
T1836 |
acomp |
normal,were |
R1133 |
T1839 |
T1836 |
punct |
.,were |
R1134 |
T1841 |
T1842 |
advmod |
However,were |
R1135 |
T1843 |
T1842 |
punct |
", ",were |
R1136 |
T1844 |
T1842 |
nsubj |
homozygotes,were |
R1137 |
T1845 |
T1846 |
dep |
that,were |
R1138 |
T1846 |
T1844 |
relcl |
were,homozygotes |
R1139 |
T1847 |
T1846 |
acomp |
semi-congenic,were |
R1195 |
T1906 |
T1907 |
amod |
incomplete,usage |
R1196 |
T1907 |
T1905 |
pobj |
usage,of |
R1197 |
T1908 |
T1907 |
prep |
of,usage |
R1198 |
T1909 |
T1910 |
det |
the,trap |
R1199 |
T1910 |
T1912 |
poss |
trap,acceptor |
R1200 |
T1911 |
T1910 |
compound |
gene,trap |
R1201 |
T1912 |
T1908 |
pobj |
acceptor,of |
R1202 |
T1913 |
T1910 |
case |
's,trap |
R1203 |
T1914 |
T1912 |
compound |
splice,acceptor |
R1204 |
T1915 |
T1881 |
punct |
.,revealed |
R1205 |
T1917 |
T1918 |
compound |
Western,blot |
R1206 |
T1918 |
T1919 |
compound |
blot,analysis |
R1207 |
T1919 |
T1920 |
nsubj |
analysis,revealed |
R1208 |
T1921 |
T1919 |
punct |
", ",analysis |
R1209 |
T1922 |
T1919 |
acl |
using,analysis |
R1210 |
T1923 |
T1924 |
det |
a,antibody |
R1211 |
T1924 |
T1922 |
dobj |
antibody,using |
R1212 |
T1925 |
T1924 |
amod |
polyclonal,antibody |
R1213 |
T1926 |
T1924 |
acl |
raised,antibody |
R1214 |
T1927 |
T1926 |
prep |
against,raised |
R1215 |
T1928 |
T1929 |
det |
a,peptide |
R1216 |
T1929 |
T1927 |
pobj |
peptide,against |
R1217 |
T1930 |
T1929 |
acl |
encoded,peptide |
R1218 |
T1931 |
T1930 |
agent |
by,encoded |
R1219 |
T1932 |
T1931 |
pobj |
exon,by |
R1220 |
T1933 |
T1932 |
nummod |
3,exon |
R1221 |
T1934 |
T1920 |
punct |
", ",revealed |
R1222 |
T1935 |
T1936 |
amod |
multiple,species |
R1223 |
T1936 |
T1920 |
dobj |
species,revealed |
R1224 |
T1937 |
T1936 |
prep |
in,species |
R1225 |
T1938 |
T1939 |
amod |
wild,type |
R1226 |
T1939 |
T1941 |
nmod |
type,testes |
R1227 |
T1940 |
T1939 |
punct |
-,type |
R1228 |
T1941 |
T1937 |
pobj |
testes,in |
R1229 |
T1942 |
T1939 |
cc |
and,type |
R1230 |
T1943 |
T1939 |
conj |
heterozygous,type |
R1231 |
T1944 |
T1936 |
punct |
", ",species |
R1232 |
T1945 |
T1946 |
dep |
one,corresponds |
R1233 |
T1946 |
T1936 |
relcl |
corresponds,species |
R1234 |
T1947 |
T1945 |
prep |
of,one |
R1235 |
T1948 |
T1947 |
pobj |
which,of |
R1236 |
T1949 |
T1946 |
prep |
to,corresponds |
R1237 |
T1950 |
T1951 |
det |
the,size |
R1238 |
T1951 |
T1949 |
pobj |
size,to |
R1239 |
T1952 |
T1951 |
amod |
expected,size |
R1240 |
T1953 |
T1951 |
prep |
of,size |
R1241 |
T1954 |
T1955 |
nummod |
48,kDa |
R1242 |
T1955 |
T1953 |
pobj |
kDa,of |
R1243 |
T1956 |
T1957 |
punct |
(,1E |
R1244 |
T1957 |
T1920 |
parataxis |
1E,revealed |
R1245 |
T1958 |
T1957 |
compound |
Figure,1E |
R1246 |
T1959 |
T1957 |
punct |
),1E |
R1247 |
T1960 |
T1920 |
punct |
.,revealed |
R1248 |
T1962 |
T1963 |
nsubj |
This,were |
R1249 |
T1964 |
T1962 |
cc |
and,This |
R1250 |
T1965 |
T1966 |
nummod |
three,species |
R1251 |
T1966 |
T1962 |
conj |
species,This |
R1252 |
T1967 |
T1966 |
amod |
other,species |
R1253 |
T1968 |
T1963 |
acomp |
undetectable,were |
R1254 |
T1969 |
T1963 |
prep |
in,were |
R1255 |
T1970 |
T1971 |
amod |
homozygous,mutant |
R1256 |
T1971 |
T1972 |
compound |
mutant,testes |
R1257 |
T1972 |
T1969 |
pobj |
testes,in |
R1258 |
T1973 |
T1963 |
punct |
", ",were |
R1259 |
T1974 |
T1963 |
cc |
but,were |
R1260 |
T1975 |
T1976 |
det |
a,amount |
R1261 |
T1976 |
T1978 |
nsubj |
amount,was |
R1262 |
T1977 |
T1976 |
amod |
reduced,amount |
R1263 |
T1978 |
T1963 |
conj |
was,were |
R1264 |
T1979 |
T1976 |
prep |
of,amount |
R1265 |
T1980 |
T1981 |
det |
an,band |
R1266 |
T1981 |
T1979 |
pobj |
band,of |
R1267 |
T1982 |
T1981 |
amod |
intense,band |
R1268 |
T1983 |
T1984 |
punct |
∼,38 |
R1269 |
T1984 |
T1985 |
nummod |
38,kDa |
R1270 |
T1985 |
T1986 |
npadvmod |
kDa,smaller |
R1271 |
T1986 |
T1981 |
amod |
smaller,band |
R1272 |
T1987 |
T1978 |
acomp |
present,was |
R1273 |
T1988 |
T1978 |
punct |
.,was |
R1274 |
T1990 |
T1991 |
nsubj |
It,is |
R1275 |
T1992 |
T1991 |
neg |
not,is |
R1276 |
T1993 |
T1991 |
acomp |
clear,is |
R1277 |
T1994 |
T1995 |
mark |
if,corresponds |
R1278 |
T1995 |
T1991 |
ccomp |
corresponds,is |
R1279 |
T1996 |
T1995 |
nsubj |
this,corresponds |
R1280 |
T1997 |
T1995 |
prep |
to,corresponds |
R1281 |
T1998 |
T1997 |
pobj |
TRIP13,to |
R1282 |
T1999 |
T1991 |
punct |
.,is |
R1283 |
T2001 |
T2002 |
det |
The,mRNA |
R1284 |
T2002 |
T2006 |
nsubj |
mRNA,indicate |
R1285 |
T2003 |
T2004 |
advmod |
greatly,decreased |
R1286 |
T2004 |
T2002 |
amod |
decreased,mRNA |
R1287 |
T2005 |
T2002 |
compound |
Trip13,mRNA |
R1288 |
T2007 |
T2002 |
cc |
and,mRNA |
R1289 |
T2008 |
T2009 |
amod |
predicted,protein |
R1290 |
T2009 |
T2002 |
conj |
protein,mRNA |
R1291 |
T2010 |
T2011 |
amod |
correct,length |
R1292 |
T2011 |
T2009 |
compound |
length,protein |
R1293 |
T2012 |
T2011 |
punct |
-,length |
R1294 |
T2013 |
T2009 |
prep |
in,protein |
R1295 |
T2014 |
T2013 |
pobj |
mutants,in |
R1296 |
T2015 |
T2016 |
mark |
that,is |
R1297 |
T2016 |
T2006 |
ccomp |
is,indicate |
R1298 |
T2017 |
T2018 |
det |
the,allele |
R1299 |
T2018 |
T2016 |
nsubj |
allele,is |
R1300 |
T2019 |
T2018 |
compound |
Trip13RRB047,allele |
R1301 |
T2020 |
T2021 |
advmod |
severely,hypomorphic |
R1302 |
T2021 |
T2016 |
acomp |
hypomorphic,is |
R1303 |
T2022 |
T2006 |
punct |
.,indicate |
R1304 |
T2024 |
T2025 |
aux |
To,determine |
R1305 |
T2025 |
T2026 |
advcl |
determine,immunolabeled |
R1306 |
T2027 |
T2028 |
det |
the,types |
R1307 |
T2028 |
T2025 |
dobj |
types,determine |
R1308 |
T2029 |
T2030 |
compound |
germ,cell |
R1309 |
T2030 |
T2028 |
compound |
cell,types |
R1310 |
T2031 |
T2032 |
prep |
in,expressed |
R1311 |
T2032 |
T2028 |
relcl |
expressed,types |
R1312 |
T2033 |
T2031 |
pobj |
which,in |
R1313 |
T2034 |
T2032 |
nsubjpass |
TRIP13,expressed |
R1314 |
T2035 |
T2032 |
auxpass |
is,expressed |
R1315 |
T2036 |
T2025 |
punct |
", ",determine |
R1316 |
T2037 |
T2025 |
cc |
and,determine |
R1317 |
T2038 |
T2039 |
aux |
to,assess |
R1318 |
T2039 |
T2025 |
conj |
assess,determine |
R1319 |
T2040 |
T2041 |
amod |
possible,expression |
R1320 |
T2041 |
T2039 |
dobj |
expression,assess |
R1321 |
T2042 |
T2041 |
prep |
in,expression |
R1322 |
T2043 |
T2044 |
det |
the,mutant |
R1323 |
T2044 |
T2042 |
pobj |
mutant,in |
R1324 |
T2045 |
T2039 |
prep |
by,assess |
R1325 |
T2046 |
T2045 |
pobj |
means,by |
R1326 |
T2047 |
T2046 |
amod |
other,means |
R1327 |
T2048 |
T2047 |
prep |
than,other |
R1328 |
T2049 |
T2050 |
compound |
Western,analysis |
R1329 |
T2050 |
T2048 |
pobj |
analysis,than |
R1330 |
T2051 |
T2026 |
punct |
", ",immunolabeled |
R1331 |
T2052 |
T2053 |
compound |
testis,sections |
R1332 |
T2053 |
T2026 |
nsubjpass |
sections,immunolabeled |
R1333 |
T2054 |
T2026 |
auxpass |
were,immunolabeled |
R1334 |
T2055 |
T2026 |
prep |
for,immunolabeled |
R1335 |
T2056 |
T2055 |
pobj |
TRIP13,for |
R1336 |
T2057 |
T2026 |
advcl |
using,immunolabeled |
R1337 |
T2058 |
T2059 |
det |
a,antibody |
R1338 |
T2059 |
T2057 |
dobj |
antibody,using |
R1339 |
T2060 |
T2061 |
amod |
polyclonal,antipeptide |
R1340 |
T2061 |
T2059 |
compound |
antipeptide,antibody |
R1341 |
T2062 |
T2061 |
compound |
chicken,antipeptide |
R1342 |
T2063 |
T2064 |
punct |
(,see |
R1343 |
T2064 |
T2026 |
parataxis |
see,immunolabeled |
R1344 |
T2065 |
T2064 |
dobj |
Materials,see |
R1345 |
T2066 |
T2065 |
cc |
and,Materials |
R1346 |
T2067 |
T2065 |
conj |
Methods,Materials |
R1347 |
T2068 |
T2064 |
punct |
),see |
R1348 |
T2069 |
T2026 |
punct |
.,immunolabeled |
R1349 |
T2071 |
T2072 |
det |
The,cells |
R1350 |
T2072 |
T2076 |
nsubj |
cells,were |
R1351 |
T2073 |
T2074 |
advmod |
most,intensely |
R1352 |
T2074 |
T2075 |
advmod |
intensely,labeled |
R1353 |
T2075 |
T2072 |
amod |
labeled,cells |
R1354 |
T2077 |
T2072 |
prep |
in,cells |
R1355 |
T2078 |
T2079 |
compound |
control,testes |
R1356 |
T2079 |
T2077 |
pobj |
testes,in |
R1357 |
T2080 |
T2081 |
compound |
Type,B |
R1358 |
T2081 |
T2082 |
compound |
B,spermatogonia |
R1359 |
T2082 |
T2076 |
attr |
spermatogonia,were |
R1360 |
T2083 |
T2082 |
cc |
and,spermatogonia |
R1361 |
T2084 |
T2085 |
compound |
leptotene,spermatocytes |
R1362 |
T2085 |
T2082 |
conj |
spermatocytes,spermatogonia |
R1363 |
T2086 |
T2087 |
punct |
(,1F |
R1364 |
T2087 |
T2076 |
parataxis |
1F,were |
R1365 |
T2088 |
T2087 |
compound |
Figure,1F |
R1366 |
T2089 |
T2087 |
punct |
),1F |
R1367 |
T2090 |
T2076 |
punct |
.,were |
R1368 |
T2092 |
T2093 |
compound |
Zygotene,pachytene |
R1369 |
T2093 |
T2095 |
compound |
pachytene,spermatocytes |
R1370 |
T2094 |
T2093 |
punct |
/,pachytene |
R1371 |
T2095 |
T2096 |
nsubj |
spermatocytes,stained |
R1372 |
T2097 |
T2098 |
advmod |
less,strongly |
R1373 |
T2098 |
T2096 |
advmod |
strongly,stained |
R1374 |
T2099 |
T2096 |
punct |
", ",stained |
R1375 |
T2100 |
T2096 |
cc |
and,stained |
R1376 |
T2101 |
T2102 |
expl |
there,was |
R1377 |
T2102 |
T2096 |
conj |
was,stained |
R1378 |
T2103 |
T2104 |
det |
no,staining |
R1379 |
T2104 |
T2102 |
attr |
staining,was |
R1380 |
T2105 |
T2104 |
amod |
detectable,staining |
R1381 |
T2106 |
T2104 |
prep |
in,staining |
R1382 |
T2107 |
T2108 |
amod |
late,pachytene |
R1383 |
T2108 |
T2109 |
compound |
pachytene,spermatocytes |
R1384 |
T2109 |
T2106 |
pobj |
spermatocytes,in |
R1385 |
T2110 |
T2102 |
punct |
.,was |
R1386 |
T2112 |
T2113 |
nsubj |
TRIP13,appeared |
R1387 |
T2114 |
T2115 |
aux |
to,be |
R1388 |
T2115 |
T2113 |
xcomp |
be,appeared |
R1389 |
T2116 |
T2117 |
amod |
nuclear,localized |
R1390 |
T2117 |
T2115 |
acomp |
localized,be |
R1391 |
T2118 |
T2113 |
punct |
.,appeared |
R1392 |
T2120 |
T2121 |
expl |
There,was |
R1393 |
T2122 |
T2123 |
det |
no,staining |
R1394 |
T2123 |
T2121 |
attr |
staining,was |
R1395 |
T2124 |
T2123 |
amod |
such,staining |
R1396 |
T2125 |
T2123 |
prep |
of,staining |
R1397 |
T2126 |
T2125 |
pobj |
nuclei,of |
R1398 |
T2127 |
T2121 |
prep |
in,was |
R1399 |
T2128 |
T2129 |
nmod |
mutant,tubules |
R1400 |
T2129 |
T2127 |
pobj |
tubules,in |
R1401 |
T2130 |
T2129 |
amod |
seminiferous,tubules |
R1402 |
T2131 |
T2132 |
punct |
(,1F |
R1403 |
T2132 |
T2121 |
parataxis |
1F,was |
R1404 |
T2133 |
T2132 |
compound |
Figure,1F |
R1405 |
T2134 |
T2132 |
punct |
),1F |
R1406 |
T2135 |
T2121 |
punct |
.,was |
R1407 |
T2137 |
T2138 |
aux |
To,assess |
R1408 |
T2138 |
T2140 |
advcl |
assess,used |
R1409 |
T2139 |
T2138 |
advmod |
further,assess |
R1410 |
T2141 |
T2142 |
det |
the,localization |
R1411 |
T2142 |
T2138 |
dobj |
localization,assess |
R1412 |
T2143 |
T2142 |
amod |
nuclear,localization |
R1413 |
T2144 |
T2140 |
punct |
", ",used |
R1414 |
T2145 |
T2140 |
nsubjpass |
TRIP13,used |
R1415 |
T2146 |
T2140 |
auxpass |
was,used |
R1416 |
T2147 |
T2148 |
aux |
to,probe |
R1417 |
T2148 |
T2140 |
advcl |
probe,used |
R1418 |
T2149 |
T2150 |
amod |
meiotic,chromosomes |
R1419 |
T2150 |
T2148 |
dobj |
chromosomes,probe |
R1420 |
T2151 |
T2150 |
acl |
prepared,chromosomes |
R1421 |
T2152 |
T2151 |
prep |
by,prepared |
R1422 |
T2153 |
T2154 |
compound |
surface,spreading |
R1423 |
T2154 |
T2152 |
pobj |
spreading,by |
R1424 |
T2155 |
T2154 |
prep |
of,spreading |
R1425 |
T2156 |
T2157 |
compound |
spermatocyte,nuclei |
R1426 |
T2157 |
T2155 |
pobj |
nuclei,of |
R1427 |
T2158 |
T2140 |
punct |
.,used |
R1428 |
T2160 |
T2161 |
prep |
In,was |
R1429 |
T2162 |
T2163 |
amod |
wild,type |
R1430 |
T2163 |
T2160 |
pobj |
type,In |
R1431 |
T2164 |
T2161 |
punct |
", ",was |
R1432 |
T2165 |
T2161 |
expl |
there,was |
R1433 |
T2166 |
T2167 |
amod |
diffuse,staining |
R1434 |
T2167 |
T2161 |
attr |
staining,was |
R1435 |
T2168 |
T2167 |
amod |
nuclear,staining |
R1436 |
T2169 |
T2167 |
punct |
", ",staining |
R1437 |
T2170 |
T2167 |
cc |
and,staining |
R1438 |
T2171 |
T2172 |
det |
no,evidence |
R1439 |
T2172 |
T2167 |
conj |
evidence,staining |
R1440 |
T2173 |
T2172 |
prep |
of,evidence |
R1441 |
T2174 |
T2173 |
pobj |
concentration,of |
R1442 |
T2175 |
T2174 |
prep |
on,concentration |
R1443 |
T2176 |
T2177 |
compound |
SC,cores |
R1444 |
T2177 |
T2175 |
pobj |
cores,on |
R1445 |
T2178 |
T2174 |
punct |
(,concentration |
R1446 |
T2179 |
T2174 |
acl |
marked,concentration |
R1447 |
T2180 |
T2179 |
agent |
by,marked |
R1448 |
T2181 |
T2182 |
det |
the,SYCP3 |
R1449 |
T2182 |
T2180 |
pobj |
SYCP3,by |
R1450 |
T2183 |
T2184 |
amod |
axial,element |
R1451 |
T2184 |
T2185 |
compound |
element,protein |
R1452 |
T2185 |
T2182 |
compound |
protein,SYCP3 |
R1453 |
T2186 |
T2172 |
punct |
),evidence |
R1454 |
T2187 |
T2172 |
prep |
at,evidence |
R1455 |
T2188 |
T2189 |
det |
any,substage |
R1456 |
T2189 |
T2187 |
pobj |
substage,at |
R1457 |
T2190 |
T2189 |
amod |
meiotic,substage |
R1458 |
T2191 |
T2192 |
punct |
(,1G |
R1459 |
T2192 |
T2161 |
parataxis |
1G,was |
R1460 |
T2193 |
T2192 |
compound |
Figure,1G |
R1461 |
T2194 |
T2192 |
punct |
),1G |
R1462 |
T2195 |
T2161 |
punct |
.,was |
R1463 |
T2197 |
T2198 |
compound |
TRIP13,signal |
R1464 |
T2198 |
T2199 |
nsubj |
signal,was |
R1465 |
T2200 |
T2201 |
advmod |
noticeably,absent |
R1466 |
T2201 |
T2199 |
acomp |
absent,was |
R1467 |
T2202 |
T2199 |
prep |
in,was |
R1468 |
T2203 |
T2204 |
nmod |
mutant,nuclei |
R1469 |
T2204 |
T2202 |
pobj |
nuclei,in |
R1470 |
T2205 |
T2204 |
amod |
meiotic,nuclei |
R1471 |
T2206 |
T2199 |
punct |
.,was |
R7276 |
T10698 |
T10699 |
amod |
Developmental,Phenotypes |
R7277 |
T10700 |
T10699 |
prep |
of,Phenotypes |
R7278 |
T10701 |
T10702 |
compound |
Trip13,Mutant |
R7279 |
T10702 |
T10703 |
compound |
Mutant,Mice |
R7280 |
T10703 |
T10700 |
pobj |
Mice,of |
R7281 |
T10705 |
T10706 |
punct |
(,A |
R7282 |
T10706 |
T10707 |
meta |
A,are |
R7283 |
T10708 |
T10706 |
punct |
),A |
R7284 |
T10709 |
T10707 |
dep |
Shown,are |
R7285 |
T10710 |
T10711 |
nummod |
21,d |
R7286 |
T10711 |
T10713 |
npadvmod |
d,old |
R7287 |
T10712 |
T10711 |
punct |
-,d |
R7288 |
T10713 |
T10715 |
amod |
old,littermates |
R7289 |
T10714 |
T10713 |
punct |
-,old |
R7290 |
T10715 |
T10707 |
nsubj |
littermates,are |
R7291 |
T10716 |
T10707 |
punct |
.,are |
R7292 |
T10719 |
T10720 |
det |
the,tail |
R7293 |
T10720 |
T10718 |
dobj |
tail,Note |
R7294 |
T10721 |
T10720 |
amod |
shortened,tail |
R7295 |
T10722 |
T10720 |
prep |
in,tail |
R7296 |
T10723 |
T10724 |
det |
the,mutant |
R7297 |
T10724 |
T10722 |
pobj |
mutant,in |
R7298 |
T10725 |
T10720 |
punct |
", ",tail |
R7299 |
T10726 |
T10720 |
cc |
but,tail |
R7300 |
T10727 |
T10728 |
amod |
overall,similar |
R7301 |
T10728 |
T10729 |
amod |
similar,size |
R7302 |
T10729 |
T10720 |
conj |
size,tail |
R7303 |
T10730 |
T10729 |
compound |
body,size |
R7304 |
T10731 |
T10718 |
punct |
.,Note |
R7305 |
T10733 |
T10734 |
punct |
(,B |
R7306 |
T10734 |
T10735 |
meta |
B,are |
R7307 |
T10736 |
T10734 |
punct |
),B |
R7308 |
T10737 |
T10735 |
dep |
Shown,are |
R7309 |
T10738 |
T10739 |
nummod |
23,d |
R7310 |
T10739 |
T10741 |
npadvmod |
d,old |
R7311 |
T10740 |
T10739 |
punct |
-,d |
R7312 |
T10741 |
T10743 |
amod |
old,littermates |
R7313 |
T10742 |
T10741 |
punct |
-,old |
R7314 |
T10743 |
T10735 |
nsubj |
littermates,are |
R7315 |
T10744 |
T10735 |
punct |
.,are |
R7316 |
T10746 |
T10747 |
det |
The,mutant |
R7317 |
T10747 |
T10748 |
nsubj |
mutant,is |
R7318 |
T10749 |
T10748 |
acomp |
smaller,is |
R7319 |
T10750 |
T10748 |
prep |
in,is |
R7320 |
T10751 |
T10752 |
det |
this,case |
R7321 |
T10752 |
T10750 |
pobj |
case,in |
R7322 |
T10753 |
T10748 |
punct |
", ",is |
R7323 |
T10754 |
T10748 |
cc |
but,is |
R7324 |
T10755 |
T10756 |
det |
the,tail |
R7325 |
T10756 |
T10757 |
nsubj |
tail,is |
R7326 |
T10757 |
T10748 |
conj |
is,is |
R7327 |
T10758 |
T10757 |
neg |
not,is |
R7328 |
T10759 |
T10760 |
advmod |
as,truncated |
R7329 |
T10760 |
T10757 |
acomp |
truncated,is |
R7330 |
T10761 |
T10760 |
prep |
as,truncated |
R7331 |
T10762 |
T10763 |
det |
the,mouse |
R7332 |
T10763 |
T10761 |
pobj |
mouse,as |
R7333 |
T10764 |
T10763 |
prep |
in,mouse |
R7334 |
T10765 |
T10766 |
punct |
(,A |
R7335 |
T10766 |
T10764 |
pobj |
A,in |
R7336 |
T10767 |
T10766 |
punct |
),A |
R7337 |
T10768 |
T10757 |
punct |
.,is |
R7338 |
T10770 |
T10771 |
punct |
(,C |
R7339 |
T10771 |
T10772 |
meta |
C,testes |
R7340 |
T10773 |
T10771 |
punct |
),C |
R7341 |
T10774 |
T10775 |
amod |
Wild,type |
R7342 |
T10775 |
T10772 |
nmod |
type,testes |
R7343 |
T10776 |
T10775 |
punct |
-,type |
R7344 |
T10777 |
T10775 |
punct |
(,type |
R7345 |
T10778 |
T10775 |
appos |
WT,type |
R7346 |
T10779 |
T10775 |
punct |
),type |
R7347 |
T10780 |
T10775 |
cc |
and,type |
R7348 |
T10781 |
T10782 |
amod |
homozygous,mutant |
R7349 |
T10782 |
T10775 |
conj |
mutant,type |
R7350 |
T10783 |
T10782 |
compound |
Trip13,mutant |
R7351 |
T10784 |
T10782 |
punct |
(,mutant |
R7352 |
T10785 |
T10782 |
appos |
MUT,mutant |
R7353 |
T10786 |
T10772 |
punct |
),testes |
R7354 |
T10787 |
T10772 |
punct |
.,testes |
R7355 |
T10789 |
T10790 |
punct |
(,D |
R7356 |
T10790 |
T10791 |
nsubj |
D,are |
R7357 |
T10792 |
T10790 |
punct |
),D |
R7358 |
T10793 |
T10790 |
cc |
and,D |
R7359 |
T10794 |
T10795 |
punct |
(,E |
R7360 |
T10795 |
T10790 |
conj |
E,D |
R7361 |
T10796 |
T10795 |
punct |
),E |
R7362 |
T10797 |
T10798 |
compound |
cross,sections |
R7363 |
T10798 |
T10791 |
attr |
sections,are |
R7364 |
T10799 |
T10798 |
prep |
through,sections |
R7365 |
T10800 |
T10801 |
nummod |
17.5,d |
R7366 |
T10801 |
T10803 |
npadvmod |
d,old |
R7367 |
T10802 |
T10801 |
punct |
-,d |
R7368 |
T10803 |
T10805 |
amod |
old,testes |
R7369 |
T10804 |
T10803 |
punct |
-,old |
R7370 |
T10805 |
T10799 |
pobj |
testes,through |
R7371 |
T10806 |
T10807 |
amod |
heterozygous,mutant |
R7372 |
T10807 |
T10805 |
compound |
mutant,testes |
R7373 |
T10808 |
T10809 |
punct |
(,WT |
R7374 |
T10809 |
T10806 |
parataxis |
WT,heterozygous |
R7375 |
T10810 |
T10809 |
punct |
“,WT |
R7376 |
T10811 |
T10809 |
punct |
”,WT |
R7377 |
T10812 |
T10809 |
punct |
),WT |
R7378 |
T10813 |
T10806 |
cc |
and,heterozygous |
R7379 |
T10814 |
T10806 |
conj |
homozygous,heterozygous |
R7380 |
T10815 |
T10805 |
compound |
Trip13,testes |
R7381 |
T10816 |
T10791 |
punct |
", ",are |
R7382 |
T10817 |
T10791 |
advmod |
respectively,are |
R7383 |
T10818 |
T10791 |
punct |
.,are |
R7384 |
T10820 |
T10821 |
mark |
Whereas,show |
R7385 |
T10821 |
T10826 |
advcl |
show,synchronized |
R7386 |
T10822 |
T10823 |
det |
the,tubules |
R7387 |
T10823 |
T10821 |
nsubj |
tubules,show |
R7388 |
T10824 |
T10823 |
prep |
in,tubules |
R7389 |
T10825 |
T10824 |
pobj |
WT,in |
R7390 |
T10827 |
T10828 |
amod |
coordinated,spermatogenesis |
R7391 |
T10828 |
T10821 |
dobj |
spermatogenesis,show |
R7392 |
T10829 |
T10828 |
prep |
with,spermatogenesis |
R7393 |
T10830 |
T10831 |
compound |
pachytene,spermatocytes |
R7394 |
T10831 |
T10829 |
pobj |
spermatocytes,with |
R7395 |
T10832 |
T10831 |
relcl |
present,spermatocytes |
R7396 |
T10833 |
T10832 |
prep |
in,present |
R7397 |
T10834 |
T10835 |
det |
all,tubules |
R7398 |
T10835 |
T10833 |
pobj |
tubules,in |
R7399 |
T10836 |
T10835 |
punct |
(,tubules |
R7400 |
T10837 |
T10835 |
amod |
proximal,tubules |
R7401 |
T10838 |
T10837 |
prep |
to,proximal |
R7402 |
T10839 |
T10840 |
det |
the,lumen |
R7403 |
T10840 |
T10838 |
pobj |
lumen,to |
R7404 |
T10841 |
T10826 |
punct |
),synchronized |
R7405 |
T10842 |
T10826 |
punct |
", ",synchronized |
R7406 |
T10843 |
T10844 |
amod |
developmental,progression |
R7407 |
T10844 |
T10826 |
nsubjpass |
progression,synchronized |
R7408 |
T10845 |
T10844 |
prep |
in,progression |
R7409 |
T10846 |
T10847 |
det |
the,mutant |
R7410 |
T10847 |
T10845 |
pobj |
mutant,in |
R7411 |
T10848 |
T10826 |
auxpass |
is,synchronized |
R7412 |
T10849 |
T10826 |
neg |
not,synchronized |
R7413 |
T10850 |
T10826 |
prep |
between,synchronized |
R7414 |
T10851 |
T10850 |
pobj |
tubules,between |
R7415 |
T10852 |
T10826 |
punct |
.,synchronized |
R7416 |
T10854 |
T10855 |
det |
Some,tubules |
R7417 |
T10855 |
T10856 |
nsubj |
tubules,have |
R7418 |
T10857 |
T10858 |
det |
no,spermatocyes |
R7419 |
T10858 |
T10856 |
dobj |
spermatocyes,have |
R7420 |
T10859 |
T10858 |
compound |
pachytene,spermatocyes |
R7421 |
T10860 |
T10861 |
punct |
(,asterisks |
R7422 |
T10861 |
T10858 |
parataxis |
asterisks,spermatocyes |
R7423 |
T10862 |
T10861 |
punct |
),asterisks |
R7424 |
T10863 |
T10856 |
punct |
", ",have |
R7425 |
T10864 |
T10865 |
mark |
while,is |
R7426 |
T10865 |
T10856 |
advcl |
is,have |
R7427 |
T10866 |
T10865 |
prep |
in,is |
R7428 |
T10867 |
T10866 |
pobj |
others,in |
R7429 |
T10868 |
T10865 |
punct |
", ",is |
R7430 |
T10869 |
T10865 |
nsubj |
development,is |
R7431 |
T10870 |
T10871 |
advmod |
somewhat,disorganized |
R7432 |
T10871 |
T10865 |
acomp |
disorganized,is |
R7433 |
T10872 |
T10873 |
punct |
(,# |
R7434 |
T10873 |
T10865 |
punct |
#,is |
R7435 |
T10874 |
T10873 |
punct |
),# |
R7436 |
T10875 |
T10856 |
punct |
.,have |
R1140 |
T1848 |
T1849 |
punct |
(,N4 |
R1141 |
T1849 |
T1847 |
parataxis |
N4,semi-congenic |
R1142 |
T1850 |
T1849 |
punct |
),N4 |
R1143 |
T1851 |
T1846 |
prep |
on,were |
R1144 |
T1852 |
T1853 |
det |
the,strain |
R1145 |
T1853 |
T1851 |
pobj |
strain,on |
R1146 |
T1854 |
T1855 |
compound |
C57BL,6J |
R1147 |
T1855 |
T1853 |
compound |
6J,strain |
R1148 |
T1856 |
T1855 |
punct |
/,6J |
R1149 |
T1857 |
T1842 |
advmod |
often,were |
R1150 |
T1858 |
T1859 |
advmod |
markedly,smaller |
R1151 |
T1859 |
T1842 |
acomp |
smaller,were |
R1152 |
T1860 |
T1842 |
cc |
and,were |
R1153 |
T1861 |
T1860 |
punct |
/,and |
R1154 |
T1862 |
T1860 |
cc |
or,and |
R1155 |
T1863 |
T1842 |
conj |
had,were |
R1156 |
T1864 |
T1865 |
amod |
kinked,tails |
R1157 |
T1865 |
T1863 |
dobj |
tails,had |
R1158 |
T1866 |
T1864 |
cc |
or,kinked |
R1159 |
T1867 |
T1864 |
conj |
shorter,kinked |
R1160 |
T1868 |
T1869 |
punct |
(,2A |
R1161 |
T1869 |
T1863 |
parataxis |
2A,had |
R1162 |
T1870 |
T1869 |
compound |
Figure,2A |
R1163 |
T1871 |
T1869 |
cc |
and,2A |
R1164 |
T1872 |
T1869 |
conj |
2B,2A |
R1165 |
T1873 |
T1869 |
punct |
),2A |
R1166 |
T1874 |
T1842 |
punct |
.,were |
R1167 |
T1877 |
T1878 |
compound |
RT,PCR |
R1168 |
T1878 |
T1880 |
compound |
PCR,analysis |
R1169 |
T1879 |
T1878 |
punct |
-,PCR |
R1170 |
T1880 |
T1881 |
nsubj |
analysis,revealed |
R1171 |
T1882 |
T1880 |
prep |
of,analysis |
R1172 |
T1883 |
T1884 |
compound |
Trip13Gt,expression |
R1173 |
T1884 |
T1882 |
pobj |
expression,of |
R1174 |
T1885 |
T1886 |
punct |
(,1D |
R1175 |
T1886 |
T1880 |
parataxis |
1D,analysis |
R1176 |
T1887 |
T1886 |
compound |
Figure,1D |
R1177 |
T1888 |
T1886 |
punct |
),1D |
R1178 |
T1889 |
T1890 |
det |
a,level |
R1179 |
T1890 |
T1881 |
dobj |
level,revealed |
R1180 |
T1891 |
T1890 |
amod |
low,level |
R1181 |
T1892 |
T1890 |
prep |
of,level |
R1182 |
T1893 |
T1894 |
advmod |
normally,spliced |
R1183 |
T1894 |
T1895 |
amod |
spliced,transcripts |
R1184 |
T1895 |
T1892 |
pobj |
transcripts,of |
R1185 |
T1896 |
T1890 |
prep |
in,level |
R1186 |
T1897 |
T1896 |
pobj |
testes,in |
R1187 |
T1898 |
T1897 |
prep |
of,testes |
R1188 |
T1899 |
T1898 |
pobj |
homozygotes,of |
R1189 |
T1900 |
T1901 |
dep |
that,is |
R1190 |
T1901 |
T1890 |
relcl |
is,level |
R1191 |
T1902 |
T1901 |
advmod |
presumably,is |
R1192 |
T1903 |
T1904 |
det |
a,consequence |
R1193 |
T1904 |
T1901 |
attr |
consequence,is |
R1194 |
T1905 |
T1904 |
prep |
of,consequence |